LOCUS       DAORYL010000001       149420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_65_length_149420_cov_12.793104, whole genome shotgun sequence.
ACCESSION   DAORYL010000001 DAORYL010000000
VERSION     DAORYL010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 149420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 149420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..149420
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65_length_149420_cov_12.793104"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..10714)
                     /locus_tag="PLV81_00005"
     CDS             complement(<1..10714)
                     /locus_tag="PLV81_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08515.1"
                     /translation="MKFVHVTVFIIFFSILATGFAGTIPFEQSSAKGYFERSLQSAMA
                     EKTKEAFIHKAMQAADTAYSIWEKEALTAYDGAIVTGEDDISGFLKTEARTAIQKMLE
                     EALGSYDARCFEIVVPEYAKEELYARINQMMQEDPHEDMVATYSAWDENVGHYVQQLL
                     AGIDSQLQTTRDALLSEINTTTQEYRKGFIETLDEKLNNIHKLIQRDIELYYVGAKNT
                     YFYQHYSDTASLRFYTQQQSASQITAQILESTTDKTDAILTEVQNKLSYTISSIGLPP
                     EGDDLDMLVQAGIYAWKAAEDDLLAARLSWERSSLDGYRQADEIWAKSFEQLVQKRNE
                     WLASIQQQIRQGIINWNDTFIETDHQYASALKLLDETIAAQKEQFDDYCASTRELITS
                     GSQSLAIALENISWLGSYFTTLTGLQYSNDPQTRSTILQKLSADTTETKELKIAVFQQ
                     LNQWYDLKVRYNDIIARMTLQYHTQDMWGTPVTIFKSGEFVKRFTGEEQAFAGAMANF
                     RALYMAVREGNIANIQSSNAFNFETGLHKYIVGMQCLPLLGSGQIVTFEELIASLKNL
                     PQYAGQPEKLEALRPFLSSLYYSYSTGEITATGQYNGPAGTDAWSIARMEAFRQFAAT
                     ATGNFEDLFGYTEVVQKTTGLLTDDRYDNEGNPLFEYYLYKDTDGAVIIDGLYQQAHD
                     PYLMTAAEFDLEKEKLTLAYWEERLRRAQKLYEYAYNETGRPDAQTQKANYQAAIDTY
                     NKAKKEYEDALALLSGQLKDALVAAQKRVDDAQKKFEIARTAYEAAQKLYQEAMERCL
                     YYSNPDAKEMAIQLVNEATQQIIAITNRIQQKEQKWITARMNYYKALMLQEHNSEASQ
                     YAQRIELTAKILEGYTTDEGQVRGLRDMYTTWQTIQNGTLDDVMAAIDDNNDILFLKL
                     ETILPGYAEDERLTFLQEALENREKYSEEYEEAKDLYIRWKAIVSIENTESRVHEAIV
                     SKLNELQANNFALTWSMLQEQIDTIANVSATVDERKAAIGVLVAYTADTVDSLELTEL
                     LDETDPETIHEKALQIFLGALLKHVYTLDITTHVDNQVVYPDTEILYAEYDKQIQDEY
                     RIRRIDLVNAINALGKSFEDLYHITRNVVVYLLHDFDTENLETSLYTKNNIIQQANAA
                     VDEASVTYFEQNKKTVEKLLHILNATKEAATDSDGIHWEYFNSTYQDLVSMCDMALSN
                     RNVREALEYGYLYTLVTTLKDSIDIHDEASFNAVTTMLEKSIEDIGIVIDVYKNELAG
                     CDDTTIDEKIKTYISMLETGKDQQGNPLSDTELHIVQVKLTAVTQPDIFDFIHPLAYE
                     GEQSVVVMQSFARYVEQFYELSLEKRRRTQDEGFTGKIAEILGIDAGQAQTMFKQSMP
                     VGQLIEYGRKAESYIQTKEYGTLPQSLKEALQAVVSAYHDVLIQKAKYENRNTDSNQA
                     SAAYEKSRSVYEKLSSLQKKYEELIALHNAMYGNQENEYTASQQNYYQLQPWGTWPGF
                     ADAIIRTLQECNVLYSQVINEINNTQYNDLQAQVFAKLAKLEETRRNYEIVDYVARYL
                     KEGEMYSSVEDYANRMRSSGKDEQFIQDVIAHITHELTKRQDIQALKENPTGIKEVQN
                     NWDESDTGWILAAYYQGIQENSEDATTFNALLYDTDFRAYALLMHFQKFIQHYIHEKG
                     FSDFDDVFGLLDGEILRAGEFLKLPQYSTDGQIQEDIKQLCTSLYEDFKQVYNTTVSQ
                     YITVQSLTIHNAYTYTASNTSPADIYAHFMAYLTSGTFDQMPAFEDVFGTIHEAGFEP
                     SGFTALTQCGTQEWQQFFVNNYPTFQMLYSKLQQDSSIIAYSVLPEGVREYAIFTDYF
                     AMAHSGITSADDAEKLLAYYGIHPDISGYDELKMHTESFIQVMDVALAYDGSMPIEEY
                     IQQNNITDPKEKNFLILYSIAPELADPVNLIVTDGQVPLVLTGYQMQTKARELFEAIS
                     NEDDNGLQSLLAMCMYAAHTNSVIAQNVYQFILSQRNTYSNWRDYIGIMEQYYEQYSA
                     DSNSPSMLYQVIIDKEKLKKRDEDGLDIAQKWYKDINANGSFDSGDKTLQDFIAEYDA
                     NGYGISRKENTSGSMEAVKSDGLEGNVKGYYTGDNKSYYNAMIDKATTMAAALDMLVQ
                     VAQMATQATDTFTFYDEDGSGIDTSFVRDIRGLLNELQDAGYDGTVSMGDDESTGSPI
                     EQALQIIKSYSQGTDDARIKQLVDSMREEDNTIQQTLLKAYADIEKMGIVLESDNLQE
                     YLESDTFQEAQSVYTANKTLFEQAQIQVDNALQAYHDAQDDYTKQLEIISILYNRLEE
                     ARKLKEDEEMLYAYASTPYLYNSQTNAEGGDPDDAASKLKADAKEQYELALKYRNEVQ
                     QRIEELKHNVEAVQQQKVEHDQEYVKLRNELIERAQRAYKTEKLAMCMQNEIKQLSVA
                     YQELKGQYEAKKDAFIAATDDEYKDERDKLIDRMLQYSFVNGITTVKRKDIDENTATL
                     VAKALVLSRGYSVYMPVGVSEAIMAKMVYDYIKDNKDRTATIGSLEYHLQQSAYYFDK
                     NAQKFGASRKIDTDPWFGSIPGGTVIADTNSPMHEYLQLLKDNKITNNALIGEYYSNF
                     LTARDAEFSYENLSIVYGGYYEVYKPIKKRFDYLWNKTIKVLGKKIHPLRPLAYVLKP
                     TVLLFELGLNKLFKPIREAEQKWKNNDHNAKAQLAKIHSKLTELQEIKLKMLQAKAAL
                     ARYTEIESLDDMQQAGAFVYYNPKTMQTVSVQKPTLKAALKEIAGKYGIMISDSDLQF
                     MVEENRQGDWVNVNNYRTTIHKTDENGYETDETVQVLHAGTVGQTYANIMNQQKRDVF
                     DRYMAHVQAMYTQGGYDRVVIDRAVEKDLYGIWAGDSSDYQSALTIGAESKGTIRAML
                     AYMGYVEGMPVDVINQLDAMISTENEQQVEELFAQYVKQYEGINDRELAQRKALQVHE
                     WQLKKQQLADKKADWDRMVSRIFQRGVKQWEMMVQSFMNRWKNWKDETEEQILEGNKQ
                     WDERMKQLQQAKLQWLADAQSGVSAKQLHKQLTGIEVLLYDMVTMMKEKYGNVISKVD
                     VHAMLLDILKEQPELLSSEIMNLSKQHTEFGLTQIRLRSYNYTIFDDANRLSKEFETA
                     QKKTDNIALLKALTELLKRCKEQIDIANENSQTAALQFVSAYNFGIDGNTYTRKLSVS
                     DKKQKIDAYRPYQYTDSLVLGTYNIPMLMDLYQKDGVAFTATMNVVLSQVQTRMGMMM
                     RPDIPWGFTYYVGEFGVIEPGPKLKNKGKGEYARITKEVYNAEKEKATEDAICQYVDA
                     GIAVTLSIATANPMVGAAYMSWNQFSQVATGDLSLQHWAVQTGISVGASYAGGYIGGA
                     TNSAVYGSLTTSAIQGLGNFVEYKQDGGLGLQWGSKQQWRSFGIGASTALVGAKIGQM
                     YGDSWNNSWQHYGYTASSSYLVNRFDGTGSWKDDLIQASVSTAALWASNELLAQGTVL
                     GNMARSTNAIAPINRFIQGTLENIILAGVQVGQGKSIYDALLEQNWAKTQYTMADYLS
                     DWASKIAQEHSEATRKMMEMEKKEGLLGLAWLGLSRAGSLLGSAWDG"
     gene            complement(10711..11397)
                     /locus_tag="PLV81_00010"
     CDS             complement(10711..11397)
                     /locus_tag="PLV81_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08516.1"
                     /translation="MAGTIKIVISSFALAILCCGFATPVHVSAATHIPSTSRLRIIEA
                     TQKASQYMAAMKAMQCIEEEAMLAMHSYTIKAGGYSFSLHNDGISPAATHIPPAWNVL
                     THSFYSHTNVMLCMVYVMLLAVAYCYERFYYRQREAYPPCEFLYHELSHIYINACGIY
                     CIPSIIFKTPVNSGHTINKSFLPMYMSLLPCSVLYCMAVSRFQHTHIKTSPLEKSVHI
                     PISGKHEVRL"
     gene            11716..12054
                     /locus_tag="PLV81_00015"
     CDS             11716..12054
                     /locus_tag="PLV81_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOM08517.1"
                     /translation="MDITKRTKDDVVILDISGEIDLYNAPEIKDIINSLIEEKKFNVI
                     INLEKVTYIDSSGIGALISSLSNLKKYQGGLKIINVFASVRKVFELTKLTSFFEIYDS
                     EEAAIESFKR"
     gene            12446..>13438
                     /locus_tag="PLV81_00020"
     CDS             12446..>13438
                     /locus_tag="PLV81_00020"
                     /inference="COORDINATES: protein motif:HMM:NF025704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HOM08518.1"
                     /translation="MHINKNVAVLAAIVCLSMITACFEKVPVKELSLAKNAITEAESV
                     KADYYAKDEYTAAKDLLLKAHDNVKNEEYDKAKQNAQESQKKAKEAYDKALPLLAKDT
                     LDIATQSLKAADDAYASVLAKEDYTKAADMLQEANKQYENKNYLAAYKAALDADLYAK
                     NARNVAMNQKETLADSIKEVNIVIAQAESYGAAQYAPEKLALAKEHSQIARSAYEAGE
                     LKKGFSAIEVAKLNADDAYFTSLKATAAKKIEEAQAAIDAAKGSEAAKLSPNDIKAAE
                     ESLVQAKSLFNDGKYKESMIASGQAINIAQGVSSKKAVAVAPIVEQQGKEGVAVK"
     assembly_gap    13439..13538
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13539..13832
                     /locus_tag="PLV81_00025"
     CDS             <13539..13832
                     /locus_tag="PLV81_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOM08519.1"
                     /translation="EEETEYTLYKVKYNPAKRDCLWRIAEKFYNDGFKWKVIYDANRD
                     IVKNPDLIKPGWILKIPKAATKLTQPVNPVKEKNTDAIKENEPPATELKMKTE"
     gene            complement(13870..14646)
                     /gene="mazG"
                     /locus_tag="PLV81_00030"
     CDS             complement(13870..14646)
                     /gene="mazG"
                     /locus_tag="PLV81_00030"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOM08520.1"
                     /translation="MSIPPLYKLKEIASILRSEHGCAWDKAQTSDTLKPYLIEEAYEV
                     YQAIENGNTENLKEELGDLLYQIYAHAQLASEQNIFDIDDVASGIIEKLIRRHPHVFG
                     NETAKDKYEVIDRWEKIKKKEKEDGQSMLNGVPVHLPALLKAYRVQQKVSRVGFDWEH
                     IQDIVVKLHEEVNEFKDALQNNKDSREALKEEIGDILFTIVNIARHMEINPEEALQHT
                     VSKFIARFQHIEKRLHEMNKDITQTSLQELDELWEESKGE"
     gene            15062..16330
                     /gene="glyA"
                     /locus_tag="PLV81_00035"
     CDS             15062..16330
                     /gene="glyA"
                     /locus_tag="PLV81_00035"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOM08521.1"
                     /translation="MGLQKYLKDSDPEVFSIIQKENQRQEHSLILIASENYTSQAVLE
                     ASSCTMTNKYAEGYPGKRYYNGCQFVDQVETVAIERIKKLFGADVANVQPHSGTQANM
                     AVLMALLEPGDTIMGMNLSHGGHLSHGSPVNFSGIYFKVVSYGVTKDTHRIDYDEAYR
                     LAKEHKPKIIIAGASAYPRIIDWNRFREIADSIGAVLLTDIAHIAGLVATGHHPSPVP
                     VADFVTMTTHKTLRGPRGGLILAKQKYEGMMNKYIFPGIQGGPLMNTIAAKAVAFGEA
                     LQPSFKEYSAKVIANAKVLADTLLSRGLTLVSGGTDNHLMLVDLRNKNLTGRDVANRL
                     DEAGITCNKNGVPFDDKSPMVTSGIRLGSPALTTRGLGEDEFRLIGTLICDIIDNMDD
                     EKVLNRVRSQVQELCKAYPTDFLRLEPRGI"
     gene            16531..17520
                     /locus_tag="PLV81_00040"
     CDS             16531..17520
                     /locus_tag="PLV81_00040"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOM08522.1"
                     /translation="MKKLVAVILMCSIAVVGCKKEKKFTVAREGLVNFVAGTVLLVSG
                     GAESKAQIGDAIKEGMTIKTVGPKSMAEIYFGENAVKILGDTVIKVEKLITNLDSNTE
                     ESSFIVEKGRMFSKVTKKLAKGDSYQVKTPTAIAAVRGTDFLVTQEEDKSNVACLDGL
                     LEVLNQSLATGQPIVLEEKQEVDVVPGQDMVAKQISEDKLRMLNILLEIKAMREDIRK
                     KYEQQREEILKYVEEQKKADKTMLEEQREKDKALVEEQKARDKAMIEGIKGEIKDESG
                     KAAQQSLDAAKSQMQDAKAVDKDQASTEAKDQMSAMKPKIEKPKVDLNQFKTQ"
     gene            18066..18851
                     /locus_tag="PLV81_00045"
     CDS             18066..18851
                     /locus_tag="PLV81_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOM08523.1"
                     /translation="MVKLAFLGTGTCNATQRKPQALAFSIDGELVCVDFGGGAYHAIA
                     SLNDSDFNYSNISTVILTHYHVDHVSGLPDLLWGEMWDHTGHRTETLTIVGPKGLHNF
                     YHDRLLPFMGDYPLPFKVELIELNNGDAFEGNGYRVEAVSLAHGDFSSGYRFDFGTRS
                     VAITGDTGYCDALQGLLSSADIAVIEWGIASDDTYPLHLSSKDIIALIKSGAWPREVY
                     AVHVYPVVDIHFQEQLQKMKSLVASLNKTIEFPSDGDILTIIP"
     gene            18959..>19470
                     /locus_tag="PLV81_00050"
     CDS             18959..>19470
                     /locus_tag="PLV81_00050"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOM08524.1"
                     /translation="MKKIETEYCIIGAGVVGLAVARELSLKKKKKIIVCEKEEHVGMH
                     ASGRNSGVLHAGIYYTAGSMKASTCIEGNRLMKSFCKSKGVPVRETGKVIVAKNSNEL
                     QTLSMLEQRAKHNGVEVKLINAKKLSDIEPCAKTYQEALFSPHTAVVDPKVVMQALYE
                     DCTQRGVNVL"
     assembly_gap    19471..19570
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19571..19894
                     /locus_tag="PLV81_00055"
     CDS             <19571..19894
                     /locus_tag="PLV81_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015852584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HOM08525.1"
                     /translation="NIVLKDALLFFANRSFRSVAIEEPKKYIPYYFYRDAKELVWKLL
                     YSDIEKTTKAGIRPQLVDWAKKELVMDFCIYHDAHTVHVLNAISPAFTSSLYFAQLIV
                     DRYCM"
     gene            19981..21315
                     /locus_tag="PLV81_00060"
     CDS             19981..21315
                     /locus_tag="PLV81_00060"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOM08526.1"
                     /translation="MKISVVGTGYVGLVTGTCFAVMGNTVICVDTDSDKIEKLKKGII
                     PIYEPGLDELVVENHKKGNLLFTTDIKEALEKTNVMFIAVGTPMGEDGSADLRHVLDV
                     AQSIGKFMTHDMIVVDKSTVPVGTAEKVKATIQEELKKRGVTFKVMVVSNPEFLKEGA
                     AVEDFMRPDRVIIGTDDEEALNVMKELYAPFTHSHERFIAMDIRSAEMTKYAANAMLA
                     TKISFMNEIANICEKVGADVNMVRIGIGSDHRIGYSFIYPGVGYGGSCFPKDVQALIK
                     TSRDYGYEPKIITAVEEVNAKQKLSLVDKVVKRFGQDLSGKVFAIWGLAFKPETDDMR
                     EAASITIIKELLKRNAKVKAYDPKATEQAQGFYLKGLPNIEYCDSKYDALTGADALLL
                     VTEWKEFRSPDFDEVKKRLKNPVIFDGRNQYNMKKLQKDGFEIYQIGVGKVK"
     gene            complement(21416..21874)
                     /locus_tag="PLV81_00065"
     CDS             complement(21416..21874)
                     /locus_tag="PLV81_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3996 domain-containing protein"
                     /protein_id="HOM08527.1"
                     /translation="MKNILLPCIFVLMFATIASAAHNFGAGFILGDPSGLTAKIFMGK
                     SDAIDIGIGESADDLYIYADYLRHFHGVFPINELVFYLGVGAGFHDWEKDRKNDHDEE
                     NRIDVRIPVGLEYTFTKVPVGIFIELVPALRIIPDVDFDIRGGLGARYYF"
     gene            22070..22525
                     /locus_tag="PLV81_00070"
     CDS             22070..22525
                     /locus_tag="PLV81_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010299248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HOM08528.1"
                     /translation="MTTRQRILRKLVNLYPPFLGAGIRIKFDDTTNKVAVSMKLTWYN
                     RNYVGTHFGGSLYTMCDPFYMILLMDILGKEYIVWDKSATIEFVKPGRSTVHAEFYIP
                     DEIVEKIKKEVDKNGKSMPEFVAEVKDEQGEVVARVYKYIYVRKKDTGR"
     gene            22526..23482
                     /locus_tag="PLV81_00075"
     CDS             22526..23482
                     /locus_tag="PLV81_00075"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOM08529.1"
                     /translation="MIYPATEIYIQRAAQAIKQGNVVAFPTETVYGLGANALNAEAVA
                     KIFEIKQRPYFDPLIVHVDSREMVQAVVATLPDIALRCMEAFWPGPLTLVLPKSTIIP
                     EIVTAGLDTVAVRMPQHQVAQKLIQASGVPIAAPSANPFGYLSPTTANHVASQLGDKV
                     EIILDGGQCPVGVESTIIKIEKDSITLLRHGGIPVEDIEAVAGKISVIRHTQVEAPGQ
                     LPYHYAPKTKIVLINDISKIPDDSSCGVILYKKRDLPFKNIHVMYLSDNGDLRQAAAN
                     LFTCLHALDAMRLSVIYAEAIPEVGLGQAIMDRLKKAQARHS"
     gene            23605..24996
                     /locus_tag="PLV81_00080"
     CDS             23605..24996
                     /locus_tag="PLV81_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOM08530.1"
                     /translation="MIQFDFNYFMDEFIGSEHGIAKKDIDDLTPQCDAARKRMDDEKA
                     KGMLGFLELPFADTIVEDIRAFKATVPWCDTVALIGIGGSALGPQALKNALADIYLNE
                     LPDEIRGKRCKAYFFDNVDPGEMQSLLKILNIPSTLFLVISKSGGTTETNANFAILLD
                     AIKKQTKDYKKHIVTITDPDKGILRQITQEEGFVSFPVPSNVGGRFSVLSAVGLVIAE
                     FLGIDAYGLLKGAARVVDRYYGKTVWENAPLLNAAFHYLFDIKKNKRINVLMPYTRKL
                     YLFADWYRQLWAESLGKRFDIQGKEVMAGLTPVAALGTIDQHSQLQLYLEGPNDKVIT
                     FLKLKDFGTDILMPQFYKGKQELEYLSGKSLRKLNEFEEQATELVLKDASRPNASIIL
                     PKLDEEAVGQLIMFLEMQTAYAGYLYNINPYDQPAVEQGKRFTFGLLERPGYEDYKEK
                     FVKGYVKKESYIL"
     gene            25010..25621
                     /gene="tmk"
                     /locus_tag="PLV81_00085"
     CDS             25010..25621
                     /gene="tmk"
                     /locus_tag="PLV81_00085"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOM08531.1"
                     /translation="MKIKTPLFIVFEGIDGAGKSTQANLLFSFCRAKTPAVLLQEPTD
                     SQYGKLLRKMLKGEIPGTRDELLDLFIKDRAYDVEHNITPSLNRGYVVIVDRYFYSTA
                     AYQAGDGIKSSDIVRMNIERGFPVPDRVYFIDIEPAIALERIHRRSGDNKEIFETLQT
                     LETIRKNYLSIADSTFAVIQGSLGVEDMFQEVLKDFEKHFVTG"
     gene            25633..26934
                     /locus_tag="PLV81_00090"
     CDS             25633..26934
                     /locus_tag="PLV81_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08532.1"
                     /translation="MRLFRQLIIQGINIIFLSLLLLLGWMLHTVKIEGNLLVIFIVFS
                     IVTIFPLYTYYIERFYKNIIPLKYEDLYIQTVDMFLSLQSFDDIIKVIFDKVLQYIGV
                     KSGLLIFYSHASDDYTIYYQKQQKQKVIRKAHIDKNNIIFKIIQSGDDILIKSKMDPS
                     LHFQRSIMFEMEKLGGEIIIPIYYHDIFLGLMIIGEMKRRISRRDILMLKALASKIAT
                     VTVNSFFMHELIKNKEIEKEYELGHKVLKQFLPPDKGIIHKTQYMMIQNPDSTITCYF
                     NIYNPDPMTSYIALCPLQANISLLSLLIPAISVLMQSYSRLGFLPDHIIKKINGVLKE
                     KELIEENLPMMIVKKKKKQYCISRSGNCNMKVYVQKKTKVMPLNLQDAINQVNNIKKL
                     IITSGWLPNNIGGIDEVDETIDVDIHSIPENNFMLILSEIL"
     gene            26931..29243
                     /locus_tag="PLV81_00095"
     CDS             26931..29243
                     /locus_tag="PLV81_00095"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOM08533.1"
                     /translation="MKKRILFYSFSIIYTLIFVLTVPYGYKNAVTYPFDRLLVGSTFL
                     YRLHDTPHALFKATLETCDSRTLNNDFQTLTDWECISTINNTTVKQHFKSNNKVALWL
                     VLILAFSSILLIVWAAGVYYARKDMFANLFILIANAVALNCLVYIDLFFVHRLHAVYP
                     AVSSLVPLCLFYIVFTMKESNQLVKTVCYVIVLVTGIVLSGTVYCNVLAFEKVYQLNG
                     IISVVLFIIAAIMLVCEHNHARFLIKRNIVVAGAIVAGGVLPFAVLLAGTYHDMQPST
                     LFSLLFTIAIPVSIGNGLLANYSLGSVANLQKNSISMVVDIIIAVVISTGLFYMYNFY
                     SILLSPPIIIGLFLAIMLIILHIRMLIIRALDDIIFLKNDEYSLSLQRIAEIITWPKN
                     LKEKLDAIENELKTLIKVKKISYAIIDNYQYNDERIIVLDEDSNLASFFKKRNETITS
                     ASFFSSRNDEKAVEEYIITHGIEACIPVCINESLKGILFVHNKGDYFTNVELNFLSML
                     ALQVHQLVINSKALTEYINSRNYEKELDLASYIQVRLFPKKPPQDCGIQFSIYSRPYL
                     KVTGDYYDIIPVDKNRVAVVIADISGHGLSAAMILSATSAIINGMLKEKKGIDKVVAE
                     LNHFLTIRYTGFELITLFIGLYNKRTRSMEYINAGHIAPLVVSKDKKLYHLEGRSKIL
                     GVDPMAQYYSSRYSFSAGDQMILYTDGLTDLYNSNSDITFGEDSLNEILNQSLTKPIE
                     EKIHAITEGIVTFGQEYIKDDITVIGMHFD"
     gene            29161..31659
                     /gene="sppA"
                     /locus_tag="PLV81_00100"
     CDS             29161..31659
                     /gene="sppA"
                     /locus_tag="PLV81_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOM08534.1"
                     /translation="MQLQKGLLHLARNISRMTLLLLACILINAIITGNAFPDVKQQAP
                     DVLMPGATVSMPGIPSYSYINPVFADINEAFSLQYRYAHINSGDIGTHYAATTLYGFT
                     IAYSTVEALFAEKEVIQTQGESWYFAKGIMLTDWLGVGVSYNRYTVNNDPVTSWGYSL
                     LLMPSRFISFGMVIDHANKPSLDEVTLKQREWYSIGIRPFGNTLSFAWDIKRYEGEPW
                     KDVLHVFTLSGMVWRDIICSLQYDMDRNISFALAIPFDISSRTGGSSLYDVGYMHFDK
                     QKATAYTFGITLTEERFRNALSSKMRYLSIVLRDQVKEVKDKSLFEPSKASFFDIVRS
                     LREAMYDESIAGILIQIDAPALNFAQAQELREEIKRCKQKGKPVYCILASMGNIEYYI
                     ASSASKIYYVPNQPFGITGLKAEVYFFKGLFDKVGIRFEEIKKGQYKSFGESFTRQQM
                     SDAARENIISLLKDLNQQYIQDIAQDRNISVQEVEQCIAGGILTPQLAKSRGFIDDIA
                     RPYEVLQRLGVERYGLSYLVTCDSYIEETMFVYEWGPKPKIAVIYVEGNIIRGEAKDD
                     SFLSPAMIGDSNYRKYLEKVFADKDVKAVVIRINSGGGSAIASDLMWHTLVAMKKRYK
                     KPVVISFGNIAASGGYYIACTNDTIFTSKATITGSIGVIFGKFNLEELYSKLGINKET
                     IKMSEFADIFSESRAFTEKEKQLIQKSVDYTYNEFVQKVETGRNIPQESIPSVAEGKV
                     FTGAQALQNKLADKEGGLIAAISYAATLAHIKGEFQVVQLPQKDIDLRKLLKGTSFQA
                     SLSGILQYVINALALETLSREEALLLYPYDIVIK"
     gene            31700..33175
                     /locus_tag="PLV81_00105"
     CDS             31700..33175
                     /locus_tag="PLV81_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08535.1"
                     /translation="MTIPKKHTIASIIVLYVVLFSFSVYAKPKAGFGIIRGYSSDENY
                     LATLIRGHCVNIATSLGIFDIINPDNLLQQLQRLPCNEEQCILQFASQADLQIIVMGT
                     VERSAQGYVVAINAYGLGMPYYGKQFCNYNTKVAVNILDRADREASYIAEEIASQFIA
                     KLCEQYTVPLTVKNNTVAGDYIISGTYTFYTLQTVYDNYTVLIPGGRVEVFNNTVATT
                     VPDDSIILINFKKEADSIKNFYYGRKKEIVFKKSQVEDSCILMAFTVPASASMPLVVP
                     LFGYYTSEDYGGLLLWAGNVVPYIGLEIWGFVNRPSELKKHHNDISRNQMASYYFAWY
                     MLLAGGMPSFVDAFSHQYLQQARLYQGQVPTMGSSTTEIYLALIGAGSGMFYKGNRLW
                     GYVYFHLTNICVYGLLYNYAKPQSWDEQNKRYTTGASHAKAMYAFAGLLSLIKIVEIV
                     HTVSVPYALSVTSEMSSIDVSPDFRINPYEKVIGISCAMHF"
     gene            33211..>33687
                     /locus_tag="PLV81_00110"
     CDS             33211..>33687
                     /locus_tag="PLV81_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08536.1"
                     /translation="MKTVYADDMPVDVVIINTSIQTPMIQALSSITQNAVKEAFEECK
                     QLVPADAESFISMTGVQAPGDIIGKAHLYGISLLVYMRVFLVGPVYYCELSFKPLDDK
                     VAFKEETVTVQGTIARNIPLKAKREIIKRHQDVLQCTIKTKIDARTYVVNAGQWHGL"
     assembly_gap    33688..33787
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <33788..34281
                     /locus_tag="PLV81_00115"
     CDS             <33788..34281
                     /locus_tag="PLV81_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08537.1"
                     /translation="VNFFPWKQDRDKTDAQYRLHVYLWATLPVTYTVAFFDQLAYAYE
                     RHRYLPPFFADKDVMAVLVSAIVPGGGLFYKGNRLWGYGYWLSEFTLGGVVAYKQAGN
                     RNILLGILAGIKVVELFHAWLATPAYMVYSYELSHNTTPINVGMSSFGNELCFYTYFS
                     KNY"
     gene            34338..35426
                     /gene="carA"
                     /locus_tag="PLV81_00120"
     CDS             34338..35426
                     /gene="carA"
                     /locus_tag="PLV81_00120"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOM08538.1"
                     /translation="MPEKAFLILEDGTTFEGTQFGWEGEALGEVVFNTSMSGYQEVLT
                     DPSYNGQIVAMTYPMIGNYGVNDEDVESSRVQVAGFVVKEYSKTYSNFRAKRSLDDYL
                     KEYKVPGIEGVDTRKLTRHIRDKGAMRAGIFLDKAGALEKVLYHPPMAGLDLTRNVMC
                     QKPYTFGTHRDTAPTIAVFDFGVKTNILRLLTAEGFNVTVYPGITPLSEVLKNTTIKG
                     VFLSNGPGDPDAVDYAKVLVKDILKTEIPCFGICLGHQLLALGLGAKTYKLKFGHRGG
                     NQPVKNVLTQKVEITSQNHGFAVDYESTKKIPDIEITHINCNDTTVEGLRHKKLPLFC
                     VQYHPEANPGPHDSRYLFKEFYSMVAQC"
     gene            35646..37943
                     /locus_tag="PLV81_00125"
     CDS             35646..37943
                     /locus_tag="PLV81_00125"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOM08539.1"
                     /translation="MSAKVQNWNEITIHSDKEPQLTENARIVLEKRYLAKDEEGNVIE
                     TPRQMFERIAKYIASADLLYGKDARHVNQVATTFYEMMVDLLFIPNSPTLMNAGRPLG
                     QLSACFVLPVEDSMDGIFDAIKYMALIHKSGGGTGFSFSRLRPKNDVVQTTKGVSSGP
                     VSFMNIFNAATETIKQGGTRRGANMAILHVTHPDIIEFIRAKEKNDALTNFNCSVAVS
                     DAFMKAVENDEEYDLINPRSGKSAGKLKAREVFELIVQMAWKNGEPGIVFIDRINEKN
                     VLKKIGLIESTNPCGEQPLLPYESCNLGSINLARCVVHNGGAAAIDYELLKDITYKAV
                     HFLDNVIDVNKYPLPMIEENTKANRKIGLGVMGFADMLIALGVPYNSEAALSIAKNVM
                     ATIQEASKEASRALAKERAAFPNFPLSEYAEKGQEPLRNATTTTIAPTGTISIIAGTS
                     SGVEPIFALAYVRNVLDNNKLVESNPLFVEYCKQKGIYSEELMQKVAKTGSVAHINEV
                     PDDIKAIFVTAHDITPQWHVRIQAAFQEFVDNAVSKTVNFPNSATVQDIETVYKLAFE
                     LGCKGITVYRDGSRESQVLQVEKEKPLERPVLKIQPRPRKEVTWGKTLKMNTGCGSLY
                     VTINEDEHGLFEVFATMGKAGGCAASQTEAVSRLISLSLRSGIEPQQIIKQLKGVRCP
                     NQAWVKGGKIYSCADAIAKAMERYINPDADQSETIDDMYKNIAETNGKGSDTVMVGVC
                     PECHGPLEFESGCSVCRMCGFSRCG"
     gene            37950..38762
                     /locus_tag="PLV81_00130"
     CDS             37950..38762
                     /locus_tag="PLV81_00130"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOM08540.1"
                     /translation="MFQVYFTADLDEELSNKLEEHAQSISILYDKKAVIALFNSEEEI
                     PACARDYILSIQTLDEEIWKYRYLDSFNGIAVTPSVFIYPATKGKMCDSSYKYTIAIN
                     PKDAFGDGTHPTTRMCIQTLESITGTIAPEKRSSYSLCDIGTGTGIIAIAAELFGFGT
                     IDAFDIDPLAIQRAQENAAYNGCNRINFFVSDIAKVRSETQYTIVVANLLSDIIEKNA
                     AAIASLVEKGGTLIASGIHDRWAQGIIALMQKYCTLVDKNIMDEWHCLVFKK"
     gene            38781..39776
                     /locus_tag="PLV81_00135"
     CDS             38781..39776
                     /locus_tag="PLV81_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOM08541.1"
                     /translation="MDSCEEIYPGIFKIVQSKPRRIAPPVNIYIIAGDDGLIFDAGYG
                     DDTSIHLAGDAINSIQNRNKNWRILRVLPSHSHPDHFSGLKQLKSHYGLKVMLTEQMA
                     SVLSNKHAYRNSYKLSTLQKYRYSTVFERISELPLQVVFRTMYKKVYGIAFLQSHDTT
                     VRPGETIAINGYNFTIIHGPGHCNDHIMLYDNKEGILFAGDNILRSIYTWLGPPRSNL
                     QEYFDTLEACLRLPNLKLILSAHGSTIHNPKERIKEIIEYRKLRTRQVYSLVANETHG
                     ITFARLLNSIYPRSLERRWLAKGWIIVTLEYLVDNHILTFEGRKIKLGANDINTV"
     gene            complement(39826..40791)
                     /locus_tag="PLV81_00140"
     CDS             complement(39826..40791)
                     /locus_tag="PLV81_00140"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOM08542.1"
                     /translation="MFSVIRGMLFFTLIALTTGCSSLGMRNIPLDELKPKYANDASRF
                     ILINDTIIHIRDEGKKDGPVLLLIHGVCASLHTWDGWVEILKDHFRIIRFDIPGFGLS
                     GQIPSNNYTPEYSVDLLNKAIEHLGIKKLSIAGNSLGGFIAWKFAITHPDKVEKLILI
                     DSVGYPQEMPWLLKFSTNIFVRPFARIGMPRYFINKAVHEVYGDQSKVTDAIVDRYFE
                     LSQKEGNRSAWIDVFMVMKDYSKRKDLSKGLTDIQCPIMVMWGTKDIWIPYTTEFPKW
                     QKDLPHAVFKVYEGVGHIPMEEIPEQTAKDAFEFLTGKKLFTAKQ"
     gene            41457..41732
                     /locus_tag="PLV81_00145"
     CDS             41457..41732
                     /locus_tag="PLV81_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOM08543.1"
                     /translation="MEECIITVKVIPKSSKNSAQLENGVLRIHLNAPPVDGKANKALV
                     ELLAEIVHVPKSSIEIVRGEKGRNKQIKIKGLNESIFIKRLEQYTNK"
     gene            41757..42140
                     /locus_tag="PLV81_00150"
     CDS             41757..42140
                     /locus_tag="PLV81_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08544.1"
                     /translation="MKKGIVYFYCVFALILLQSCSSDSENIRKEGCINKNTVRIVVLL
                     TKNSLPTGKLNNDIVSQEAVKIADQRCQTIISNAMAINKKQYVKSDSHVVSRKLVLVE
                     KRKDGYMVIVDYTLDDEIASLLQCR"
     gene            42151..42972
                     /locus_tag="PLV81_00155"
     CDS             42151..42972
                     /locus_tag="PLV81_00155"
                     /inference="COORDINATES: protein motif:HMM:NF017266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MnmC family methyltransferase"
                     /protein_id="HOM08545.1"
                     /translation="MQFKALTTEDNTITVVDDTYNEAMHSLSGAYQEALYMHVIPSKV
                     LTKQGRVYVLDIGFGLGYNVLALCAEALKYNHTVFYAIISLEKNTDILSAMEYVYFHD
                     ERDTIYELLKQSARGETVMTDYFSHSVLYGDARQLLSHLPVNYFDAVFHDPFSPAKNP
                     ELWTVDFFKKLYSLCNDECIVTTYSAAMHIRTAMKQAGFIVGKGPEVGRKKEGTIATK
                     KNMHIVPLDDSYWLYLETNPKAVPFTDPTLCETPEEIRKGRKLHMAAFKKGLQAP"
     gene            complement(42947..44659)
                     /locus_tag="PLV81_00160"
     CDS             complement(42947..44659)
                     /locus_tag="PLV81_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08546.1"
                     /translation="MKKIDKTKENIKVDELDERTRKQMFEKFVQAGGKVIQEREKPTT
                     RNSKTIKPASRVVRQSQVKTPPKESKQPKIQSDEIFETWLQKKLSRLLIRLRLFFLGV
                     TSLSAEYCKPRFMDNLIHTYKTALLQFQVQYYHIFNPQYSKQVIAKLDQINPLYYELI
                     VMISNIFDRTRFNEIEALQQSNDMVHVQKLRNYLLPMYKSLYILYPYIELIELAYRNA
                     LEIQASVENQKSTSYEKRKYIRNSLYITFYKFLPRLHILFCHYYNVYIDITSPYIRTI
                     LQINDSDLPGKRKAVKPGEKEEEQQQTQEEQKELSEIPQHIAKGLSLMNISTNDIAAL
                     FSRDSYVQANPADKAIITYALFKKFDDEFSIILTTNKIKYNIQFTDAGKIDYKNKLTD
                     CYNELRKVTESFEEYKHVLETYERIRAEKPLSNAQYIEYTKRLTQLEKKKKVESRNMR
                     MTTKAIMDRIAAELDVLIRDMDEENKIIENPQDFLEFDVNVEAVHLLQNKKVYEAIHL
                     AYFYACALSYRLSEGDLAGDIEFKDGEVPIGIGQQINVTGTVSQEEKKQNQTSVIKEL
                     EDLF"
     gene            44783..45178
                     /locus_tag="PLV81_00165"
     CDS             44783..45178
                     /locus_tag="PLV81_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOM08547.1"
                     /translation="MVTFQLQTTRKEEFIDITAKVAEAVAKENVKSGLCCVYIPHTTA
                     AVTINENADPTVPKDILMGLSHLKLESLHFAHVEGNSPAHIKSSVIGCSTIIIVENNC
                     LQLGTWQGIFFCEFDGPRTRKVMVQCIGT"
     gene            45320..46177
                     /locus_tag="PLV81_00170"
     CDS             45320..46177
                     /locus_tag="PLV81_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOM08548.1"
                     /translation="MMNYFTGTEDYVISQELKDNVNVSIALGRPLLVKGEPGTGKTLL
                     AHSIAKGLGKRLLVWNIKSTTKAQEGLYVYDTVQRLNDARFGDKDVSNIKQYIKLGKL
                     GEAFASEDQVVLLIDEIDKADIEFPNDLLHELDIMNFYIPETGETITAKHRPIVIITS
                     NSEKELPDPFLRRCVFHYIAFPDEELMEKIVRVHFPNIEKKLLRECLKKFYWIRQFDM
                     LRKKPSTSELVDWIQALMVGGVPVKKIEDELPFIGTLLKKKEDIDVVVNSGKTHYAYN
                     SSIRTRDVY"
     gene            46167..47357
                     /locus_tag="PLV81_00175"
     CDS             46167..47357
                     /locus_tag="PLV81_00175"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOM08549.1"
                     /translation="MFINFFFNLKAQGVPVSIHEWIALHEALALNLNDTSLTKFYYMA
                     RAILVKNEVFYDRYDIAFLDTFKDVETTDELLEKILEGLKKVKELHLTEEEKRQIEKL
                     NLDEILRNFEEQLRQGHYKNHVGGNKAIGTGGRSTQGAWGYNPAGIRIGQGVSRHKSA
                     VQIAEKREFKNYSNQVVLDTRQMRVALSHLRSLLPIGPEEKLDVEQTVDATSRNAGEL
                     ELIWKKKEKKASKLILLMDVGGSMTPYARLVERLFSAASSQISKLKYYYFHNCVYQDL
                     WKDIERSESISTADFLKNADKDYKLIIVGDAEMAPSELTWVNGAIDYWYHNDIPGIVW
                     LQRLRETFKHSIWLNPIPKRSWYYIQTTRMIKNIFPMFELTLEGLDEGIKHLVSGKAL
                     QKVV"
     gene            complement(47413..>48360)
                     /locus_tag="PLV81_00180"
     CDS             complement(47413..>48360)
                     /locus_tag="PLV81_00180"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM08550.1"
                     /translation="QQKKKMNENYYEGIQLYKKGNYAQAKERFETVLSIDPEHTGARQ
                     YLIITTEALQKRAQKYYDAGINYKRKGDLENALLQFLEAEKRDPEYKDVKTQIANIRN
                     SSYAKKKYDYYYSQAKKQFDKKQYITAYKSANNAEIFNPDNIELVVLKKRIKNELDNA
                     SYPYTSKAEEARKKNKFDTAKTYCNKALKVNPWDEKARNILKDIYRMENLNKMYANGE
                     KAYRNGDLISAYRLFKRVDNEEPGYRNTSNYLSTIKSKLEGNINTYYNNGVAYYEQDN
                     FEAAIAEWDKVLLIDPDHQKAREYRERAVAKLELQKTLR"
     assembly_gap    48361..48460
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            48829..49794
                     /locus_tag="PLV81_00185"
     CDS             48829..49794
                     /locus_tag="PLV81_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOM08551.1"
                     /translation="MGNGNCVVQVRDIVKTFTMKKGALGKKSIVKAVDRVSLSIVEGT
                     TYGLVGESGCGKTTLARTLLRLIEPDSGEIDILNTPLHRLSKDGLRRFRKNMQIVFQD
                     PYGSLNPRMTVEKIVSESLRIHQSFSKEEVKEKTYNLLQMVGLDAGHASRYPHEFSGG
                     QRQRICIARALALSPSFVVLDEPISALDVSIQGQILNLLNDLQETLGVAYLFVAHNLA
                     VVKHISTRVGVMYLGKIVEENVSDKLYKKPLHPYTRSLIASIPDIKPSKHSFSVVKGE
                     IPSPENPPKGCHFHPRCQYAMPVCSEAYPATVTVHGAKVACFLYQ"
     gene            49776..50627
                     /locus_tag="PLV81_00190"
     CDS             49776..50627
                     /locus_tag="PLV81_00190"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM08552.1"
                     /translation="MFSLPVKTAMFVLLSCSFCFASDELTGTIAQNPKYTIYEKDGVL
                     FCTGNTEAALHNNKGAEKLVANDLQGAIDIFKEGLKHAPLFYPFLYNCGVACMRKREY
                     DAARIYLEKAMHIVPENPKIYILMGELYALTGREGDALILFRKALQINRKELQAIIKI
                     GNIYVDRNQLELAVTFFDAALKINPQYPDAVIGYAKIHFKKGEYYKALIWLKSIDVAK
                     GDYDKAYHYYFAESSFKLKDYKTAYEQYEKLLQFKNDVFFLTHSYALIEHKMNLSRQF
                     AELEHVE"
     gene            complement(50682..53774)
                     /locus_tag="PLV81_00195"
     CDS             complement(50682..53774)
                     /locus_tag="PLV81_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08553.1"
                     /translation="MERDILQSIKLEITKNLKFTPYLRICLHPFIAQSKSDIAHNILC
                     AELSAEPAIRFSAIRTITHYKLPGFTDLFHALFQHSITDDEKIQICTYLATYGNSQTV
                     ELLNNYIMDNFNKESNHTIVIQCLETLRILRHPDSKLLATLKSIINETGTHEVIRYYA
                     IRALSIYDDIHVLSSLINQNEYTLLGIFDAIAFISDYCITKKTQKNEASDSSKEENLI
                     IELRVFLSKMLPHFDEFSTRVKISCLNALITSKHRETNDYVLKILGGQNENEKEELLL
                     LLQHTIMLLRDPEPLIRALISYGTVSPHHNTIIVDTIINYFESFQSDRSSTLLKDKLF
                     NYFTVTLDSFFELYRKNYMISDVEEKNYPEIFREVRNFILLKFSPQILNRIIHYLKHE
                     KNDEIHKIITLLTTYLPFIDSSIRDPFSSLVEMLYDRDPKSREITASRLETIDFEKRF
                     LQERIIRLCNIIAKLNIQSAATLLVKIYNYLKKYRDEKLFDACIQTLSTMKYPYMLGE
                     LELMLLSGDRNDQLFSLKYLAHYTDQQAASILFELLKNTANLDPEVIVTALHLLLQDE
                     TAQYKNSSEILKNIALTYNDIAIKQAAIRNIGHCGDEKEMEWLITLFTETNEISIKET
                     ILQSISSIIPRLRDFNKRALSQFLLDCLKESGIRIRIYAIAILLQLNNKEVAKYIKEM
                     LIIKNRDIQTEMLYIFHNYNTPEFSYFLLSLLKEEYAIAPETVSILQNLPAEISEDIV
                     NFIGNMYRKNGIDISQAEATLTIKPGKIDTLSDFFIITITTYGDTNPILLEKLATTLN
                     TIQSLILNHCKKNNLVIHALLPGLITVYSNNPLNIADALIAIVQNIQQYNDTSHIPFK
                     AIIQSYNARVIQSGQDIIIVSDAQYAHDILYNHAIIDEKLYSTIHEEFTCNPLPHILA
                     NHAHIPLFYLSNKKNSLVEAHKALDQIILNEKTKKEKEQELLEEIKKRKLHIQSQGSA
                     DYLATLERVNGILRSEINEINKYIQKRSTDRELNTQVSRMLENLQRKIFLEISNFTMK
                     "
     gene            54034..54474
                     /locus_tag="PLV81_00200"
     CDS             54034..54474
                     /locus_tag="PLV81_00200"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08554.1"
                     /translation="MKLNESLELIINNVAHDLGYSVYDSVLVLHGKTPRITVKIDHIN
                     GISHLDCEKFSKELSHRIDEGQLLDDYTIEISSPGIKRELRTIEDFLRFTGAPVKGIY
                     QEDDARKTFKGTLVGIEGEIVTINETNKVLTLSYSQIIKANLDY"
     gene            54486..55811
                     /gene="nusA"
                     /locus_tag="PLV81_00205"
     CDS             54486..55811
                     /gene="nusA"
                     /locus_tag="PLV81_00205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOM08555.1"
                     /translation="MSGNFFEALHQIATEKGITREMIEEIVQSAMLSAYKKQYGITKP
                     VDITFDRERNTVKIISRKMVVRVPKNYAEEISLEEAQKIKPDAKFGDEVIVEENPLET
                     FGRIAAQTAKQVILQKIKEAEKNIVYDEYKDKVNELINGYVQRKTKEALYIDLGKTEG
                     IIPAREQSPLEHYKIGDRVKAIVLSVQKNSKGPSVVLSRAHPKFVEKLFEMEIPEIYD
                     GIVKIVNIVREPGMRTKVAVTSDRDDIDSVGACVGMKGVRIQSIVRELEGEKIDVIEY
                     SPNKKVMASNALTPAKVKEIIETRDGGVIAVVDNDQYRLAIGKNGHNARLAAKLCGFN
                     IDIKTEDQYREFLSSSESRALVEQLFATTSEGETPLEELPGLDARTITLLEAGGIFSV
                     EDLVEKSVEDLMNIDGIGEKTAQKIINIIQEYVDFEEEDYEDEQEDKQE"
     gene            55938..58421
                     /gene="infB"
                     /locus_tag="PLV81_00210"
     CDS             55938..58421
                     /gene="infB"
                     /locus_tag="PLV81_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOM08556.1"
                     /translation="MKAIDVATKNHVSTDDVIKICKDLGIPCTDDQSELANDDVFLIE
                     KKIQIIKEQRAQEAKKLIQQAELKKKIKLKRKVHVAKELKKEAEAKEDEATEEVVEHK
                     EDSKEVAPKHRAETGVVEKEKQEKKERFDKKERHGKPKDRFPKKDFRKDDKQRPGAEE
                     KEQRPERQHAPTVVEKRDVPVIDKEVVDLQKRQKEKGKAKEKDRDKDKRFLKKEKEEL
                     EEKLLLLKRKKESQPREAVAPKEIEITETITVGDLAKKMNIKASEVIAKLMSMGVMAT
                     INQVIDADTASILASEYGTEVKVVSLYEETVIKVDEEDRPQDYVSRPPIVTVMGHVDH
                     GKTKLLDAIRETNVVATEHGGITQHIGAYRVKVEDKYVTFLDTPGHEAFTTMRARGAS
                     VTDIVVLVVAADDGVMPQTIEAINHAKAANVPIIVAINKIDLPEANPNRVKQELTAYE
                     LIPEEWGGTTLFAEISAKQKINIHELLELILIQAEMLELKANPKLRARGVVIESKLDP
                     GRGPIATVLIQNGTLHVGDPFVVGLYSGRVRAMFTDTGEQVQEATPSMPVEVLGISGV
                     PSAGDPFQVVESEKYARQIAQKRQELVRLESAQKVKKVTLEDLNTMIKEGEIKELKIV
                     IKADVDGSVQALRDSLQKLSTNDIRVKVIHAATGGINESDVMLASASNAIIIGYQVRP
                     SAKVAEIAEKESVSIKYYNIIYDAINDVRAAMEGMLSPEMKEEITSTGEVKQVFKISK
                     VGTVAGAIVLTGRLKRSDNIRLIRDGVVVYDGKLNSLKRFKNDAGEVEAGQECGFTVE
                     NFNDIKEGDNFESYQIVEIKKKLEDSSRG"
     gene            58431..58805
                     /gene="rbfA"
                     /locus_tag="PLV81_00215"
     CDS             58431..58805
                     /gene="rbfA"
                     /locus_tag="PLV81_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOM08557.1"
                     /translation="MGFRKQKLETQIKKLVGTLIVTEIKDPRIGFVTVTNVELSKDYA
                     YADVYVSVLGDENTKKKSMAGLQSARGFIQYRVGKALSIRTIPEIRFHLDTSIEEGVD
                     MVNLLEKLEKESSKNNSGSSDT"
     gene            58809..59750
                     /gene="truB"
                     /locus_tag="PLV81_00220"
     CDS             58809..59750
                     /gene="truB"
                     /locus_tag="PLV81_00220"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOM08558.1"
                     /translation="MSVPHIKDCVLLIDKPSGYTSFDIIHRIRKTLKVKKCGHAGTLD
                     KFASGLLIVCLGWATRLVPFVMGMDKRYIATMQLGVATDTHDSEGQVISKSNIQCTVE
                     DIQSCLQTYFTGEIMQVPPDYSAIKINGKRSSDRVRKGELVAIAPRPVHIHKITVIDF
                     DTACSQLTLDITCSKGTYIRALARDLGLRLHTGAHVIALRRIAIGDFSVTDALTVEQC
                     IELSHKNIEDMRGIVTPFRALGNFGVIAVKPDSLIKIKHGKLLGYDDITHVAGEPVFR
                     VVPEGTEDLVALAVKDEKGYRCKKVFIEELDKIDTMK"
     gene            59880..60137
                     /gene="rpsO"
                     /locus_tag="PLV81_00225"
     CDS             59880..60137
                     /gene="rpsO"
                     /locus_tag="PLV81_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006447003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOM08559.1"
                     /translation="MAEKSEIINKYQLHDKDTGSAEVQIALLTERINHLTEHFKVHVK
                     DHHSRRGLLKLVGKRRRLLDYLKKKDLTKYRELIKSLGIRR"
     gene            60180..62267
                     /locus_tag="PLV81_00230"
     CDS             60180..62267
                     /locus_tag="PLV81_00230"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOM08560.1"
                     /translation="MNKEFVAHIGDDVILFNTGILAKQADGAVAVSCGETIVFASAVA
                     SKKVVEGQDFFPLTVDYREKHYSAGKIPGGFIKREGRPSDKEILTSRLADRPIRPLFP
                     DDFVNEVQVIIYVLSADREHQPDVMAINAASAALIISGIPFKGPVGAVRVGRINGQWK
                     INPYFQEMEHSDIDLVVAGTKKAVTMIEGQAKNLTEDEMIEAVEFAHANIIKLCEVQE
                     ALKEACGKPLMSYEPKLSDKELEKVIRERFFADIEQLKDVHEKKAREQAKDTLITTIT
                     SQLQEQFPDTIKLVSEIVDNLDAEVVRSRILNEGKRADGRGLKDIRPIDIMIGILPRA
                     HGSALFTRGQTQSLGIVTLGTVADSQRIDSIDLIEESYKRFMLHYYFPPFSVGEVGRV
                     GGVGRREIGHGMLAERALEYAIPDEDVFPYTIRVVSEILESNGSSSMATVCSGSLALF
                     NAGVPMKAPVAGIAMGLVLEGDRYAILSDIQGIEDHLGDMDFKVAGTATGITAFQMDI
                     KIEGITTEIMRKALEQAKEGRLYILEKMNEVISEPAKELSPHAPKIAVMKIEIEKIGE
                     VIGPGGRVIKKIIEESGADINIEPDGTIFISASEQDAIDKAQLSIKGLVEEVEVGAIY
                     KGIVKRITDFGAFVEILPGKQGLVHISKLDHSRVEKVKDILNEGDEVLVKVIGIDEQG
                     RINLSRKDAMKKH"
     gene            62276..63538
                     /locus_tag="PLV81_00235"
     CDS             62276..63538
                     /locus_tag="PLV81_00235"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOM08561.1"
                     /translation="MVHKFTLPNGLTVLLEPIDNVVSVSCGLWIKVGSRHEKANEMGY
                     AHFIEHMLFKGTDKYSAKDIARLVDRVGGQHNAATNREYTCYYINVVSDYLELAVEIL
                     SDTYLHSTFDEEEIEKEKNVVIEEIRMYEDTPDEHIHDLFMDTMFYNSPLGHSILGSF
                     NTISSINRESIVSFYKHHYTDNESLFVISGNFNIEEARQIIEKYFCKDCTATNNISIP
                     SLPKYERKLRVHLDKDLEQVHLCIGTDGLSKVDEDRWAYYLFNTILGGSMSSRLFQNI
                     REKEGLCYSIYCFHSSFMDTGVFGIYCGTAPSQYNKLLQLIANECRLITSQGISEEEL
                     ADAKSYLIGNMALSLESTEVRMSHIAKNEIVFGQQFTFKEIVRNIQAVTMEDFRRITH
                     RILDNAQFSLVTIGKLPQNPINEFHLGI"
     gene            63692..64045
                     /locus_tag="PLV81_00240"
     CDS             63692..64045
                     /locus_tag="PLV81_00240"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOM08562.1"
                     /translation="MKQSPTYILDRIIHQKKEERERIRCELLEKVHTVIGELHSVIAF
                     KEAYIFGSIIRPYSFTENSDIDIGFIGLDDKDLFKAMSFLSARLGYEVDVIQIERHRL
                     GATILSEGLQWKKKE"
     gene            64027..64488
                     /locus_tag="PLV81_00245"
     CDS             64027..64488
                     /locus_tag="PLV81_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08563.1"
                     /translation="MEKERIVLLKSSIEAQIYLIDKIYDKIEERKKKRAKWVVENIGY
                     QLHNLYCAFEDLFKIIAREFENNIDNGSKYHYELLKRMAIAIEGIRPKLISEDTFPLL
                     DNLRSFRHFFRHAYSYDLDKRKIDIVLEDALKLKELYKHDIERFLKELESE"
     gene            64626..65162
                     /locus_tag="PLV81_00250"
     CDS             64626..65162
                     /locus_tag="PLV81_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOM08564.1"
                     /translation="MLDALITSKTRLKILLKFFINPSTKGYLRELAAQMNESTNAVRV
                     ELDRLVEAGYLERNANGNKVYYNANTGHPLFKDIHNVVKKYLGIDVLIDSVLAKLGKI
                     HEAYIVGDYAKGVDSGIIDVVIVGTVDYSYLAQLIEKAEGIIKRKIRPLVLSPKEKTK
                     YKEKLNLDKALLVWHNGK"
     gene            65152..65652
                     /locus_tag="PLV81_00255"
     CDS             65152..65652
                     /locus_tag="PLV81_00255"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HOM08565.1"
                     /translation="MESNWYAVYVRSRHEFKVHNALLAKGIESFLPVVERLRQWKDRK
                     KLIPFPLFPGYLFVKLPDDKVYFLHVLKINGVVKILGDSSGYQPVAEDQIVSIYKMVN
                     SKLAIDEHPYLKEGQKVKIVTGPLSGVQGILQRKGGEQYVVVSIDILQKGVAVKVNIQ
                     SIEPVI"
     gene            65729..66130
                     /locus_tag="PLV81_00260"
     CDS             65729..66130
                     /locus_tag="PLV81_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HOM08566.1"
                     /translation="MNYIKMYVTALILFLIIDALWLTVIAKNMYKQYLSYLMGDVVWW
                     AVIIFYVLFIIGLQYFCIIPAINAQSAMHAFYAGALFGLVSYATYDLTNQATIKGWPV
                     LITCIDLLWGAFIGATVSALTTKIALLLKWH"
     gene            complement(66176..66745)
                     /locus_tag="PLV81_00265"
     CDS             complement(66176..66745)
                     /locus_tag="PLV81_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08567.1"
                     /translation="MKKNILALLFTLCITAIPSLTFAIADIAVIGGYTPYGNIEFYEE
                     RYDSLKGYNYGVMGHLNVDTEVIMFGFGFYFHGEQLHYTFNGDTRDFTVKQSWGPDII
                     IMITASEIIAPYVRAGIALLSELKYDYGYDKKEKLRFLNSGWWAIGSGIHITEQVLVF
                     GELQRLSMSHDNEHSSKRYTVNAGAMFNF"
     gene            67005..68153
                     /locus_tag="PLV81_00270"
     CDS             67005..68153
                     /locus_tag="PLV81_00270"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOM08568.1"
                     /translation="MNIPFHVPYIDDSDLEAAVSAIKSGWTTMGPKTIEFEESFAQYT
                     GMPFSVAVSSCTAALHLALKVCNIGPGDEVIVPVNTFVATAEVVCYCGATPVFADIDR
                     DSHCISVDSLYRLLSPKTRAIIPVHFAGTPCEMDVIDDIARQHNLYVIEDAAHALPSW
                     YKNRLAGTIGHVGCYSFYATKTLATGEGGMLVTHNEEWARRAGRLRLHGMNRDAWRRY
                     DRGGSWQYDIEEMGYKYNMTDIAAAMGLSQLKKVEMLQKKREAIAQRYNDAFSENDAL
                     IPYVVNKGISSWHLYPLKINLEALTISRDEFIVKLEECGIKTSVHFIPLYRFSYFVKR
                     YGNQESMFPDSEWVFAREISLPIFPSMTDEQIDYVIDCVLSIAKQFKR"
     gene            68156..69976
                     /locus_tag="PLV81_00275"
     CDS             68156..69976
                     /locus_tag="PLV81_00275"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOM08569.1"
                     /translation="MKKVLYTIKPLAVVFFVADMLCIIAGYGIASLLFSYSLDAFITP
                     AVLHAFLTIAGLYITQSYRILWRYATMQDYYRIIEGFTGATVLLLIYSSVVNLVSNRV
                     LFLASAGGLALLMVYRVIIRETKPQKIALPKKKSKIILIAGAGEAGRTLLAEFKRDGR
                     GDSIIGFVDDDTRKVGTLLCGKPVLGTTYDIPSLVEQYGVKECIVAMPSAPQKEIDRV
                     IKIIRSSGNVSISILPALTKLFEKALTPELRGIGVEELIGREEIEIDSAAIEAYCKGK
                     TVLITGAGGSIGSEICRQLLHFKAGKIIAVGRGEHSIYNLKNMLDDYAKYFEKPEIYY
                     YIADINDAVRMQKILTKHKPHIVFHAAAHKYVDIMEQNPQEALRNNCLGTRTVLEVAR
                     AAGVGQFVCISTDKAVRPVSVMGATKRMAEIVTLAQNSPDMRTCCVRFGNVIGSRGSV
                     IPLFYQQIAKGGPVTITHPDMTRYFMSIPEAVMLVIHAAMLSNGGEIFVLDMGKQYKV
                     EEVARNLITLLGYEPDVDIPVVYTGIRPGEKLQEDLWNHGEELVPTLHKKIYKIAHNG
                     FDALSVTKIKELTPDYVQSLHEEECKQLIQAIVPDYRPFN"
     gene            complement(69969..71042)
                     /gene="mnmA"
                     /locus_tag="PLV81_00280"
     CDS             complement(69969..71042)
                     /gene="mnmA"
                     /locus_tag="PLV81_00280"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOM08570.1"
                     /translation="MKIAVAMSGGVDSSVTAALLVEQGHQVIGLTAKILLCAEMDGCQ
                     PRYDVCCSPESIHDAKKVATQLRIPHYIVNVEDEFSQNVIDPFCAEYIKGRTPSPCIN
                     CNTSIKFPELLNFAKELGFDMLATGHYATICKSNRYYIKRGVDTDKDQSYFLFNLSQD
                     VLKHLMLPLGQSTKREIREKARELGLATAHRPESQEVCFVQDNDYPRFIEQRLGFCPP
                     HGDIVTTDGKVLGRHKGIHHYTIGQRRGLGIAWEHPLYVVAIDAIHNRIIVGSKDELL
                     AKGLLATQVNFMKVTIAHNTPVWVKTRSTQKPFKAHATYENDILTVTFETPQASITPG
                     QAVVCYDDDGAILFGGWIDKSIN"
     gene            complement(71190..72992)
                     /locus_tag="PLV81_00285"
     CDS             complement(71190..72992)
                     /locus_tag="PLV81_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08571.1"
                     /translation="MKLSPIIMTFLIIPHILFAENYWQRYADSLIKEQKQAKEQPYIA
                     PFVIEYLDTRIAMAQELSRSFDSTLQNNNEHQEHITNCCKQALSLCFDKTLIDITKEQ
                     VTKELQHSDISNNDAIIIHTELILSSIFRQFDTIFTNNSTQQYDIKQIIQRCQPPFDD
                     LSQSTRFQELSAHANFITQQQHFITSIASLCNTTQYDDEQIRQNPEHAKALLAQLENA
                     LINVPEKTATYQQTDGIPYQITIPQQLDCARAIYEIQNKRDAIVTEKDATKIDDIESI
                     ARSYITPIENQIAEQKKLLVIMKSTDGVAVENEEAFNNVVHRFETQSKLLRDYANATT
                     LYCQLSLLQAPHINYSSRCQHIVYVASHIQKLVQSLGDSSKEIIPEVKQLFEVLKAFL
                     YTDAPKENNDERATTLQTLHTIKENIYTIASTRKDDTPNPALCNLEIAMNIETLEKGI
                     KLFNTQTYAKQALMRYASTIQKAFELVQTGFSDNTIQQIIEMQSAIPVVDNFDVQQII
                     NEYTSQQYVLRKLRADSASLMQRIESYKKKGIRINDYEKAKEIVETIKSMQSLYTVDV
                     GKYKMNQNNIIIIDRQCVAMLKRMMKKNIAAGKI"
     gene            73152..73478
                     /locus_tag="PLV81_00290"
     CDS             73152..73478
                     /locus_tag="PLV81_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOM08572.1"
                     /translation="MTIDYQKIEEAAKLLELGDRASLQQVKHAYRKLMQKWHPDHCKE
                     DKKLCDEMTKKLTEAYKLLIEFCYSYRIPLTEQKLVEELGNEDPEAFWKRKFGSDPHW
                     GGPGYR"
     gene            73556..73924
                     /locus_tag="PLV81_00295"
     CDS             73556..73924
                     /locus_tag="PLV81_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HOM08573.1"
                     /translation="MYIFELIAEQRIREAMERGEFDNLPGMGKPLPKDELDNVPPELR
                     MAYKILKNAGIVPEEVELRKEIYSLQQLIDACYDDEEMKGLVKQLSAKQLRYNILMEQ
                     KGIHVTDYEYRKKIMEKLSK"
     gene            complement(73965..74318)
                     /locus_tag="PLV81_00300"
     CDS             complement(73965..74318)
                     /locus_tag="PLV81_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08574.1"
                     /translation="MSTQVSFSKLEKEFLPQFREKISTSEDIIDVQKYFSYAIKEMLQ
                     KAFEKEEITITEEDIELTTIPPYYTIKKIDSSIQSLWEHSDLKDILQRFAEVAHKRYL
                     HLQKNPSKTNKKIRR"
     gene            74658..75626
                     /locus_tag="PLV81_00305"
     CDS             74658..75626
                     /locus_tag="PLV81_00305"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOM08575.1"
                     /translation="MRKGISIVLGLLCLLSCAKDITKVTIAKTSVPKNFTATDIKVIS
                     YNGNDVSCMLFAHRFSQGNLVYFEIVPAFDVEKITVMFNEKGIPTTKRSWGFRGFFAI
                     APDQKPGTHNFTVTIGTKKIDIPVVVVRTPFSVSTIPMDLGKFSKKEYLESPEVLEFI
                     RQCTEKKQKAFATHYNDLIRGRAFHPRDVHEVNSPFWAKRVYKLHDSISGKTQTVERI
                     HRGIDLNGNTGDPVYAMLDGIVVLAEMLFYEGNMVIINHGNGMFSYYMHMDKLYVASG
                     DTVVAGQLIGAVGSTGMSTGPHLHVSLVVDGVQVNPMSVVMLPIRD"
     gene            75761..76258
                     /locus_tag="PLV81_00310"
     CDS             75761..76258
                     /locus_tag="PLV81_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08576.1"
                     /translation="MAKPAEYFIKSKNLDEFRNDIIACDGNFDFDIEEMIALGSAYLE
                     RFPDCFSNRSCQDVQLGYQLARICLVEKLIIDFPSDVKDAFRKMFFSAQAVNEQMEYL
                     KQKLTYDELSNFVDTIQKRLEEYHLKVVSLPKGMIKERFVGGITNLFNIAYLLKMNVA
                     QKAHE"
     gene            76251..77306
                     /locus_tag="PLV81_00315"
     CDS             76251..77306
                     /locus_tag="PLV81_00315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM08577.1"
                     /translation="MNKEFLHSISHELKSPLNSILTLSKVLLMQADSRLTLEEKEYLE
                     IIERNGQKLLALINDLIEVFEIDSGIQLSVTDVSLSSVLHNVCERYEGIMRKKGLQLH
                     MHCDDIMLNTDEARLYQIFENLIDNAVKFTEEGSITIAAYKKDGHCVVSITDTGIGIR
                     SEYCDALFSGFTRASGSLSTKYPGTGLGLTIVARLVALLGGTVAVDSQLGKGSTFTVR
                     LPLQKEHEDLQQQRKTILVIDDDPDSIVVVKALLSKLYDIVETHSGQEGLDLAQDLKP
                     ACILLDLSLPDLHGLQVVKQLKENEQTSHIPVIIVSASSLKDERRTIMASGCDDFIQK
                     PFAIEEFVLKVQRWVTQ"
     gene            77303..80155
                     /locus_tag="PLV81_00320"
     CDS             77303..80155
                     /locus_tag="PLV81_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOM08578.1"
                     /translation="MSTILVIDDKKDNLVSITALLKNLIANCEVVTAQSGKEGIEKAE
                     KHNPDVIILDIKMPEMDGYEVCQILKNNPKTRHIPVIFLTAIHTDTQSKVKGLKLGAD
                     AFLTKPVDQAELIAQINAMIRIKHAEDMLRKEKDLLEDIVLQRTSELLASQMRYKSLF
                     NSISDMVIIHRRDGKILEVNNTTCDRLGFSHDAMMLMTLQQLTAEGDSDRINSYLQSI
                     NETADPYETSFVDRNGTTIPVEIVSTIIYYLNENAILSVARDITERKHIEHVRNQLSI
                     VLDKSDDAIIGATLDGKIASWNKAAQHIFGYAFDEVVGCDLAILSPPYRPDEFDQLLL
                     IIKNNEAIDHYETEGLDKKGAIHNLSMTISPLVDEHDSIIGCSIIARDFTDVKRTQEK
                     LKKGLDIIQNLEDIAPAYFITLNEEGNILTCNKAMLQATGYSLSQVQGKTCCDIFIPD
                     VGQKSFRNQLSTMKRTRATRIFESPINTKSGEEKIVQWHARPVYSDTEFDYFMFIGID
                     ITEKKQLEKKILQANLEERNRIARDLHDGLGQHLAGIIFKTEMLRLKIKNDYPQEANE
                     IVEIQNMVHQAIEQTRLIARGLSPVDLSTKGLYSSLQDMIADVNKNFAIKTILEWNIT
                     SNIDEAIDTVHVYYIIREALNNAVKHASPKNIAITISEDNAYYYVKIMDDGKGIPDEI
                     DLAKGMGMHIMQYRAWIINASFQVQNNKSGGTLVQCIIQKSTQKAVIDKPKTGKKYTI
                     VIVDDHPIVRQGLQQLINTQKNLEVIGQATSADEAIDVVNRKIPDCIIVDISLNGTSG
                     IELVKALKQRFPKLPALVLSMYDEKLYAERAIKAGARGYLMKQEAPDKIIQAIYTVLK
                     GDVYLSSEMKEQMVQSLSDKGTMDSSVEALTNREFEIFQLIGHGLGNKHIAQKLHISV
                     KTVENVREKIKMKLHLETSQDLLQYAIEWVRENSGN"
     gene            80241..80468
                     /locus_tag="PLV81_00325"
     CDS             80241..80468
                     /locus_tag="PLV81_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOM08579.1"
                     /translation="MKTIAKITRKGQVTIPKKIREMLGSDIIEFKVHRNDIIIKPVKS
                     IGGSLHKYSKRYVPFEDIRDTVWDEVVRKNK"
     gene            80465..80845
                     /locus_tag="PLV81_00330"
     CDS             80465..80845
                     /locus_tag="PLV81_00330"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOM08580.1"
                     /translation="MIYYIDTNAILRYLLKDNIEQFNKANVIFEKAREGDLYIVVLES
                     VLVECIYVLMKFYKVPKNEIVSKLETLLNYKGVKNSDKTELLKALQIFKEKNIAIVDC
                     IVYSKAKQSQSNIFTFDEKLLKLK"
     gene            81019..82989
                     /locus_tag="PLV81_00335"
     CDS             81019..82989
                     /locus_tag="PLV81_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOM08581.1"
                     /translation="MSYMPGVCTFCGTGCGHYLKVDGTSVQGVFPSRVHPVSKGRLCV
                     RGWNIHELLGTQERIIKPYIKDSGQANYQDAIARLVSALSSYKPEEIAFYASPRSSNE
                     DNFALMKLARLVFKTNNISLQAESGHRNSLDVLYNGTGFAGMLGSLEDIRKAEVLIVA
                     GMDITTQNPIIGSELHYAARSGSLLITIDSRMTKIAKLSKKFINIKPGTMRIVFDAIA
                     HYIDKQQWSDTKSAHYEEYKKYLATVPFDSIEKETGVLPAEIAFVAERLVKAKSAAVF
                     FTSGISGLSYDTVASIYNLFALAGKIGKEGCGVNPIAGINNLQGSFDMGCAPDLLTGF
                     QSINDATAKKNFEKKWGQELPQFKGKTVEQLLADSSSPLKALVIVDHDDGIVKYADRL
                     KALPFVAYIGAFTNKVADFAHVVLPITTYIETDGTFTNAERRVQLSVQKCDNAFDVLP
                     MWKLCDTIAQAAGKPFNYQSASEVMDEIASLTPSYEGISYKLLSKKHGVQWPCTKKNH
                     DGTKYCVPEKVSFVLPQKPYAAVQATQEYPYLLMLGKAQHYWHQNNIMHKTFIPLREY
                     NATLLLYPDGYVALASEDAKKIGVRDRWPVKVVAEKASMKAMVMISDDVAPGTAYVPY
                     FIREGIASFLLAYSDIVSQGEEATIPVRIEKV"
     gene            82991..84043
                     /locus_tag="PLV81_00340"
     CDS             82991..84043
                     /locus_tag="PLV81_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOM08582.1"
                     /translation="MYLMKKNDVKKIAKALEEKYTLYGPYVDEAFGQCFFDKVTADNV
                     DLNASIPTNPPKHVVFPHLETIMQYKYDKTTKKADIAMVFDDTPKALFGLRSCDLTGL
                     QCIDRFFLGQEFVDEVYRHHRSRMFIVANTCVVPFKQCFCVCTDSGPAAQEGYDLNLT
                     NLGDSYLLEVGSEKGKAFIESFGFAKATEEHLKQKQKLVEKSITMFEDIATESKAWVS
                     RVMNRVTTGFIKEEVWEYIGNQCIECGACSYVCPSCTCFNINDANTIAGNTERVRTWD
                     SCSYEGYSRMAGGHNPRKPIEDRRNKRFFCKLSYSQSKKYLRPGCVGCGRCAWVCPGD
                     IGLPNVVTYIRREIHE"
     gene            84053..84886
                     /locus_tag="PLV81_00345"
     CDS             84053..84886
                     /locus_tag="PLV81_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HOM08583.1"
                     /translation="MSEQKIVTVPEIATIEEIKDEIVDVKTFYLRFDNKEIDGNFKFK
                     SGQFIMCTVFGAGEFAVSLPPSPENDRFHITVRRIGKVTNALHDLQVGDKVGIRGPFG
                     NGFPFEEIKGKNVIYVAGGIGLIPLRSSIVHVLQHRNEFGRILLLYGSRSPQDLMYQY
                     MLDEWQKIEGFETFITVDNGTPEWKGNVGIITTLFDKVEIPVENTVAFVCGPPVMFNA
                     VIKELMQRGIKDDMIISTLERHMKCGVGKCQHCAIGRTLVCTDGPVYTYRQIKTLGEQ
                     I"
     gene            84883..85389
                     /locus_tag="PLV81_00350"
     CDS             84883..85389
                     /locus_tag="PLV81_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOM08584.1"
                     /translation="MSWLQTMYTMFKDTTCIVGVGNYYRSDDAVGLYIVDALAAKVVQ
                     PHVVIINVEDVIESYVFAIAESNCKNVLIIDAIEADAEEGTVVFGKLSDFVETGLDVS
                     THKLALRMSCKILEASGKDVYMLGIVIKHNDFGKGLSPIIQNNVDSIVSMVQSSIMSN
                     QKECVYEH"
     gene            85379..87238
                     /locus_tag="PLV81_00355"
     CDS             85379..87238
                     /locus_tag="PLV81_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOM08585.1"
                     /translation="MNIDLYGMLLQAFAILLGASIIVPLIASKRKLAGWINTIAVAIA
                     AGLLMYVAYMAIFVQQAAETQAIYFGSVPVYFLIDAFSGFFIAIISFMAVMSALYSID
                     YMEHYKDYSLVPYYINFPLFILGMIGIVTVDDLSIGFTVAWQLMTIASYFLIRFEYKE
                     KQNVRNANKYLILMELAWLFIVVGAFLINGSAMGDSIHTLTQKLGTSGGMIPVVYALL
                     LLGFSFKAGVFPFGQLWLPDAHSIAPSPVSALLSGVMIKTGIYGIVRTFFWMIPHEGH
                     TVNPLVWGSIIATFGVVTLFIGTSQSLKQSDAKRLLAYSSIGQIGYIVLAIGAGLYMY
                     HSSNMFVQMLSFLVIIGMLYHVLNHAIFKGLLFLTAGSILYETGTKDLNKLGGLLKFM
                     PVSAVIAGIASLSIAGVPPFSGFASKWTIISATLLGGSQVFFLALFGIIALFTSALTL
                     ACYVKFFGMSFASTGVEWNVEHKPQEVSWKMLVPKIILGLLCIVQGIVPFIYYETIMN
                     IFRSSTGSIVQNSFNELSAHNAVFTNALGVTINVPGMQYANAIAVPLVVIVIVIIGFA
                     VAYMLKKSAGSQEKVMPTWLCGYQDLNNNNRYKDRHMFAALKSMLWWTGGNVK"
     gene            87267..88940
                     /locus_tag="PLV81_00360"
     CDS             87267..88940
                     /locus_tag="PLV81_00360"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOM08586.1"
                     /translation="MPSIDQVKKDLKEKFKSTIKGFETPLETRLFVDVDKKDIRAICN
                     AVIDMGARYIVSIGYDNIARDNTLGLVHTFSFDKDNIFCCVRTKVPADKPVLDSITPD
                     IPNAGWSEREYMDLLGMQFEGHPKPKRLILADDWPKGVYPLRKEVPWNLMPPAAEDVA
                     YQLDEAPEGCTTVPIGPFHPTLHEPAHFAVYVDGETIKGCDYRGFMTHRGIEKLCTTQ
                     VTYNEVPFIAERICGICGSVHATSYSQSVELAAGIKISRKAEYIRTFMLELERVHSHL
                     LWLGVAGHLIGFDTVFMHSWRVREPVMWLAEKITGNRKTYGMIIIGGVRRDILPHKED
                     ILNVMDKVEKELIAIKNAIVGDTAIHRRTKGVGYVSKEDTIKWSLVGPVARARGVDID
                     TRRDHPYAAYDAVQFDVPVVDTCDVWGTVLVRILETFESIKIIRQVLRDMPVDEPLLY
                     EFDEPIKPLVHSISAVEAPRGEVVHYLITGEENRADRWRVRAPTYPNLQGVPLMLLNN
                     QLADAPIIIGSIDPCFSCTDRMALVDLKTGRQSILTNEELERLSRSKKV"
     gene            88953..89852
                     /locus_tag="PLV81_00365"
     CDS             88953..89852
                     /locus_tag="PLV81_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOM08587.1"
                     /translation="MDILLIVLNIVVFLGLAPLYEGIIRKLTAKVQSRQGPPIYQPYL
                     DLLKLLGKDNLVSDNWAFKLAPMMAFAAVTAVVTIVPIGFKANYLTHYADVITIVYLL
                     TLAGISILLGALSSKNAYAMIGASREMITMIMVEPILAMTFILGAVRMKSLAIDASMF
                     SILSTGYSWSVVLMLVVYLMALQAFVARQPFDISEAEVEILEGPFIEYSGPNYALFKY
                     SMMLKQMFYAVLFVTVFMPFLHTGLFVADLVLQLLAVAVIFVLIALLGSTNPRLRIDQ
                     AVKYYAVLIVISLCTIALSVYGI"
     gene            89877..90422
                     /locus_tag="PLV81_00370"
     CDS             89877..90422
                     /locus_tag="PLV81_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOM08588.1"
                     /translation="MWLTSKIKQLFVVTKPGTVTLPYPFEPRPVPENYRGQPYWDHHK
                     CIGCGGCANHCPARTILVRDLCNELRVMMYDGSRCTYCGRCADLCPEKAITMTPQYEL
                     ATDDKRDINETMELFMLTCQRCGRCFDHEIKNAIDKLAYLGYRYDNLEMRAVIPVSSQ
                     TFDKELYEKTKTYKRPEKIGE"
     gene            90425..90955
                     /locus_tag="PLV81_00375"
     CDS             90425..90955
                     /locus_tag="PLV81_00375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOM08589.1"
                     /translation="MLGRLAKKAFPKSLWVYHCNSGACNGCDIEILNVLTPYYDVERF
                     GIKLVASPRHADVMLISGAVTRPTFPLVKKAYDAMPSPKLVFGIGSCATGGGCWFDTY
                     NVTGGADKAIPVNYFIPGCPPRPEAIIYGVALALGLVDKKAAPIELKQIEFPIDIYQR
                     TKAWEERNVIYQLLYD"
     gene            90980..91381
                     /locus_tag="PLV81_00380"
     CDS             90980..91381
                     /locus_tag="PLV81_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM08590.1"
                     /translation="MAKKILLVDDDRDLVESLKQALTMNGYEVEVAYSGAEGLKKLLS
                     YHPDLMILDVMMETDTAGFEISYQIRSNREDSKYKDVKNVPIIMLTAINQVTNSRFSL
                     NEEQSFLPGINDFLTKPVRIDELLEKIKKYV"
     gene            91562..93511
                     /locus_tag="PLV81_00385"
     CDS             91562..93511
                     /locus_tag="PLV81_00385"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOM08591.1"
                     /translation="MRKRFATQIRFKLFISILSIVLLSGIASTFVGIRTIEKHVVGQA
                     FDTVLKDLETADFIYNSRIALKSKMLKHIALLEYIQLAIIHNNRQVLINKCKEVQKEL
                     GIDIVNITDAQGKVIARANNYSFGDDVSDDNHISYVLKNKAPCEGTDIVTHKHLEREG
                     EALAKKVIIDIVPTPKSRKVERTKVVDALAIRATIPIFYNNNFIGIIYGVRILNNYNG
                     IVDQIKELVFKDEKIGGLEVGTSTIFLDDVRVCTNVKKPDGSRAIGTLLSEEVYNKVI
                     LQGKVWWDKAFVVNNWYISAYKPLLNVNNKPIGILYVGILEKKFDEIKTSSTVNFVVI
                     TILIAMVAVGISLYLLNMIIQPVRTLVKASESVINGNYDAHIEIESEDEIGYLCYTFN
                     QMIDAIKERDRKLKEQTQNQILQSEKLASLGRLASGIAHEINNPLTAILTYSSLLLED
                     LKDTDFEEDLRVIIKETFRCRDIVRNILDFARSTKPDIKRTNINNLINEVLLILEKHV
                     NFHNIVINKELDPDVPDMYIDENQFKSVINNLAMNAADAMPQGGTLTVKTLYNLHNDS
                     ITIIVSDTGTGIKEEHLDKIFDPFFTTKEQGKGTGLGLAVTYGIIKRYYGNIDVQSEI
                     GKGTIFTITFPLAKNKEEYAVFQKA"
     gene            93524..93910
                     /locus_tag="PLV81_00390"
     CDS             93524..93910
                     /locus_tag="PLV81_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM08592.1"
                     /translation="MKDILARILIVDDEDIVLKSCLRVLQKLDYEIDTAYSGQTALDN
                     LEKKKYDIVVTDLMMPGMDGMQLLEEIKKRYPDVIVIIFTGYATVDTTRQALKAGAFD
                     YIPKPFTPDELRNVIDNAVKSLKNKK"
     gene            complement(93948..95789)
                     /locus_tag="PLV81_00395"
     CDS             complement(93948..95789)
                     /locus_tag="PLV81_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOM08593.1"
                     /translation="MIAVQNLAKRFGSTLLFEDVTFTIGSKEKVGLVGRNGLGKTTLL
                     KIIAGIEQPDEGSVSIPRHYTIGYMSQQLYFTKPVVRDEALSLLPESEQHEYWRAEKI
                     LSGLGFTTSDMLKRVHELSGGFQVRLQLAKTLILNPDMLLLDEPTNYLDITSIRWLAR
                     FLQNWPGELLFITHDRGFMDSIVTHTLGIHRNRIKKIKGNTQQFYEQIALEEEIYEKT
                     RINDERRKKEIEEFISRFRAKARLANLVQSRIKTIEKMQKREKLVPLKNLDFEFRYKP
                     INAKYVLQVDSISFGYDETPLIHNLSFSVAANDRICIIGPNGKGKTTLCKLLAGSLQP
                     QSGTITRALGVETGYFEQTNISTLNPNATIEDEISYSDPDNNKQRARTICGSLLFEGD
                     DALKPVSVLSGGEKSRVLLGKILVKPINLLILDEPSNHLDMEACDALLTAIDSFEGAV
                     ILVTHNELFLRAIAQRLIIFQNNTISIFEGDYERFLEKVGWVSESESTRTGDTDQPES
                     KINKKELRRIRSQLLQQKQSECRPLQQRIEYIENEIMQYDNLLKQCNADIIIASQSQK
                     SSEIQRLSIEINKLEKTLSSLYDELEELMELHEEKNRHYDRLIQEYA"
     gene            complement(95786..96265)
                     /locus_tag="PLV81_00400"
     CDS             complement(95786..96265)
                     /locus_tag="PLV81_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOM08594.1"
                     /translation="MVYVYSIIIIIILILLKRMIKIVPRDSICIIEKKLKYHKTLQPG
                     LHIISPFVSSRIVPVEKREGVPVSQEIQFGDSSKQRLTVRTTFLLWDPYTPFFTFEKL
                     DQEMSNKALQELSKLAAGYTKQEFEKNVDVVKQKCLESLNQFGKEYGIEFTSLDITE"
     gene            96538..98118
                     /locus_tag="PLV81_00405"
     CDS             96538..98118
                     /locus_tag="PLV81_00405"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOM08595.1"
                     /translation="MDVEPIRILVLDDDPFIGKMLATMLAGYNVKNTTSFGQFQQVIE
                     EFNPDIAIIDLHLPDAHGLDVCKWIRSQNQYEDVILYILTATNDEQTLIRAYSIGVMD
                     YIIKPFNKFILFSKLRRCALMIEIKRKLEHSLEYQKDIKKRLLQLNDFIKKCIKIDDI
                     DTLVALFLHIKYIIPLDGLFIFNKLTTGKYTVLKLISHESWQSLIPKLHKVIGQNTIE
                     PVSFFFESTTKVYIASISLQRYGYVFFVSNLPYTPDERNLIGLLAEIIRVIISRTEIY
                     EYVQKQNKIYRDEISKVRQIQVAQLPKFRNINGYEIASTFLPAEDISGDFFDGFYVDK
                     DVFQIVLCDVSGHGIASSYIGNAMRTLIRTFSFNEQSPAKVLYKVNRQMIADGKGLYY
                     FGTAVICRLYPDGTILYTSAGHPPLYYYNYNAHKIEELPNTGGLLGVFEDMAYQDATI
                     MLQSGDMLFLYTDGLTESMNPALKTLYGDQRLKDTIFINAHENPVELLHSICGSMYEF
                     TEYAPLEDDVTLICIKKI"
     gene            complement(98115..98894)
                     /locus_tag="PLV81_00410"
     CDS             complement(98115..98894)
                     /locus_tag="PLV81_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08596.1"
                     /translation="MLQDNEIETLITKLRNKYDEYAHKYSPRWFNKEAFEERLQAALR
                     SKIDLEAFIIAEIAHFETIRKRYEEKKSESSFSKKVDTLIEELTAKIKKYPKIEFHPK
                     AHFEIMHMYGACYQLLEYYFPVLWIILEDRTKLYEFEQRLQYLCAHSTTRNSKRIEDH
                     IALLQRPSVKYIEIEKDKNEYLKECAFLLHEIHQWLDTVLPIYSNTQSISFARLYVQE
                     DKRKQIITFFNNDTPQSAIKKVQNYISGIIDDFRLQAFRKS"
     gene            99087..99968
                     /locus_tag="PLV81_00415"
     CDS             99087..99968
                     /locus_tag="PLV81_00415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM08597.1"
                     /translation="MAIIQIPDQCKDPFSFETVISLYQHIINQKAEGLDLSHVTFVDP
                     YSLLCLLLIGRNYLRLTGNKLTLHRIPAQILQYMTRMDFFAHNIVDCDDTLEKHKHLK
                     RSTTSSRLLEVIEIPGKEKESVRVIAKVIATFRKRALVILKHWFTLTMVDYLVTVISE
                     ICQNIFEHSLDSGFIAIQSYMYLNERIFRIAIVDSGIGIPGTFEYMPEIKTMSNAKLL
                     QTVLTTPLSSKRRYGYGLCQVNAIMEQLKGSIFLRSENAYISVLHHRRSAKGSYIFLK
                     DDCPSFPGTQFSITLIG"
     gene            99973..100527
                     /locus_tag="PLV81_00420"
     CDS             99973..100527
                     /locus_tag="PLV81_00420"
                     /inference="COORDINATES: protein motif:HMM:NF025574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4325 domain-containing protein"
                     /protein_id="HOM08598.1"
                     /translation="MVIEIKKLATSVVVTRKSTDLFTRYVGKLLFQKIQEKLKHLAEH
                     EVVIIDFDGIRSVDASFVDECIVPLLELSQTNAFPFYIKLVNITDNVEYIVNQVIGMT
                     HDQKRYIVMTDRLCKNGCHELGSISEMEKDIIEYCVINKQATSADIASFMHVSEAEAR
                     DSLLRLYEIRAVRKIHDDTVFQAI"
     gene            100619..101083
                     /locus_tag="PLV81_00425"
     CDS             100619..101083
                     /locus_tag="PLV81_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08599.1"
                     /translation="MKQIIKKKESEDDSLFGLLASYKNLYSYMLVLESQEGTIAIIIA
                     KNPSSFDELIKTKYQQYKPVRRIKMGVSRLFVKYISPEYIIKFYGPLLGLKIPENKVH
                     IINNLENGYFILEVPSHYKEQVTQCIKRIIGLTPQKKYEIIEKSLADQEVEV"
     gene            101084..102097
                     /locus_tag="PLV81_00430"
     CDS             101084..102097
                     /locus_tag="PLV81_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08600.1"
                     /translation="MPDNTQYDSFSDQTQLINKALKYTNGNLEKARQMAAGMLQDVAV
                     IKGRFRAGKVGAFYIFLNVEYNYIININSLVTSYSDIYNKIRIFDAWKQFYNLFGDIV
                     GDLGGATEDSYKFTNHLADAIEGYDIYEPAKAQNIQVVSELFEEIIGKYFSQNTECQI
                     EIDKTSSLTLEIENIPMELPGKQEQKQEELDPDEIKMREIESQVEYVIPGTVIVSPVK
                     GKYINDIKAGEKITVMLSGKDPVSDKIARMFNAITTDGQYLPVKARIKEKVSLATGGF
                     AIYALVAKNVLVKIIEEENVKIETDKQEQKKEESNENMLFVYIALLLGLLIITGFIVF
                     ALL"
     gene            complement(102104..102346)
                     /locus_tag="PLV81_00435"
     CDS             complement(102104..102346)
                     /locus_tag="PLV81_00435"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOM08601.1"
                     /translation="MAAIKEKIIIKNTTRKGRKKSSYLPPDYKTLQEVIDGIERDYIL
                     HTLELTNWNLQKTSRVLDIARNTLKAKMKKYNIDLQ"
     gene            complement(102664..104334)
                     /locus_tag="PLV81_00440"
     CDS             complement(102664..104334)
                     /locus_tag="PLV81_00440"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOM08602.1"
                     /translation="MHPALFIRDLYLALVVYVDSRQIYSFWDTDANFSKDFIPKKSHF
                     IALPDSAAGQNIYFKIQSNYHLIGITGPVLLGTEYTLIKNIILSDIANIIIASIAFFI
                     GFFSLTIFIINRKQWQYFYFGFFALLLILYILNYTSIRDLLLDTPLLWICLWLLSSVW
                     STTMFIGFTKVIFNYQKKSILGILFVFNTYYAIFETIVLLAGIGELYFTGNMHASKYI
                     LGVRYIFQYLLIVDVIIIISVMINQFKAGNKEAGILLAGLIVLCITVLHSVVVAIGYL
                     TYDFRSYIHWGLFFLLLSLSFILIRQYTLLQTSIALSKADMNTARRIQQSIIPAIPPL
                     HQNLRISSLYIPAEIVGGDFFDYIIISQHEIGIFIADITGHGVSAALIASMCKIAFHS
                     SSKLCSNPSALLENINVILADKTAGQLLSALYVYINTSNNTLIAASAGHPRCIIINRK
                     TKDYNELPTKGRIIGAFDRLNCNNIIHQLDHDQRIILYTDGLIETCNPSGEMYGEDNF
                     FNLLIQTLHMLPEDTCLQIKKDIYTWAANEQNLYDDMTIILIDVIDFT"
     gene            105568..105639
                     /locus_tag="PLV81_00445"
     tRNA            105568..105639
                     /locus_tag="PLV81_00445"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:105601..105603,aa:Arg,seq:ccg)
     gene            105611..105922
                     /locus_tag="PLV81_00450"
     CDS             105611..105922
                     /locus_tag="PLV81_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08603.1"
                     /translation="MSGFDSRGERILAFYVYDCINKNSKQWHKKILLHKSVLVVAIVT
                     IIAGITAANAHALRTVGFITMLVVILHSVTGLALGYAAASLLGLDKSVRKTIVFINTK
                     Q"
     gene            complement(106037..106228)
                     /locus_tag="PLV81_00455"
     CDS             complement(106037..106228)
                     /locus_tag="PLV81_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08604.1"
                     /translation="MQALTGRKIEFRLFPFSIRKLQSIYSPLEIDRLLPQFMIYGMYP
                     EIVLNKENTIILLKNLAFR"
     gene            106451..107059
                     /locus_tag="PLV81_00460"
     CDS             106451..107059
                     /locus_tag="PLV81_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOM08605.1"
                     /translation="MFAIFQKGGPVMWPILLCSVLSLAIFIERLYVLIKSRPYVWDSI
                     NVFKQQFITNALNKKTIPECPGIVKDMADVLDLEKDYTIDYELHEAEHKLVRYATFYV
                     RNLEQRLNWLAIIGNIAPLLGLLGTVTGMIKVFMGIQNMQGQVNPSALAGGIWEALIT
                     TAAGLIVAIPTIIAYHYFEDRIDDITGALKELISDILEVIKQ"
     gene            107056..107484
                     /locus_tag="PLV81_00465"
     CDS             107056..107484
                     /locus_tag="PLV81_00465"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOM08606.1"
                     /translation="MSAERLFIERRKKVVIDLNIAPLIDVVFQLLLFFALTSYYVANP
                     GIEVSLPKANSAVTVQRNNIEIYITGSNAIYCNATKVELSALKAMLSTLSAASKTVIL
                     KADKTVQLGMVVQVIDIIKQTQVKDLVIATSMEENNASQK"
     gene            107468..108022
                     /locus_tag="PLV81_00470"
     CDS             107468..108022
                     /locus_tag="PLV81_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOM08607.1"
                     /translation="MPHKNRNIVYYAFFISIAVHGVFLCVSFGGRTTDIQKHTLNVTM
                     HTYTILPHVEHAADTTRLKNNPAKPVKEGGVMASQYMVSNEDSQTVTMQYTDMIRQRI
                     QESLVYPATARKDGIEGKTYIKFTIDKNGNLLSAIMVRSSGSALLDDAALKAIHSASP
                     FPAIPDTIGRERMTFVQGLTFTLK"
     gene            complement(108024..109178)
                     /locus_tag="PLV81_00475"
     CDS             complement(108024..109178)
                     /locus_tag="PLV81_00475"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOM08608.1"
                     /translation="MRKTWDKSGKNTMTHTLTIQRYANNGYGIGFIDGKTVFVPYSAV
                     GDTVAVTITRQAKNYCFASITDIITPSPNRITPQCPAFTHCGGCDFLHIPYKEELTVK
                     KELFMNTLTHIGGLSHHILPAIELISGERFHYRSHATLQSDGTHIGFYKKDSHSVEPI
                     PAKGCLLLHEKLNTFILSQSCDSHANEPIKIAIDAQSSICSDPTAVITEQECGITYER
                     SVSTFFQANKFLRSKMLLVVDELSQSFASILDVGCGVGFFSIYLAKRMKGTGIDTNIQ
                     SIEWARYNAKRNNVTVDFHTVSIENYHPFKHTHECIIIDPPRQGISKRGRKTIVAMNP
                     SCIIYVSCNPVTFARDIKSFVDSNYRLQKLYMIDMFPCTHHTEVIGLLTK"
     gene            109497..109931
                     /locus_tag="PLV81_00480"
     CDS             109497..109931
                     /locus_tag="PLV81_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOM08609.1"
                     /translation="MRLSTRSRYGVRMMFEFALKFNQGPVFLKEVAKMQGISFKYLSK
                     LVIPLKSAGLLQSVRGANGGYMLAKHPSEITIRMIVEKLEGDVSLVECTENPKVCKRN
                     VDCVARTIWKKADDAIMQVLDGITLQDMVDEYKHAAGSIYEI"
     gene            109955..110869
                     /gene="cysK"
                     /locus_tag="PLV81_00485"
     CDS             109955..110869
                     /gene="cysK"
                     /locus_tag="PLV81_00485"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOM08610.1"
                     /translation="MQYTTIIESIGNTPLVKINKLAPGGVNLYAKVESRNPLSSVKDR
                     IGYAMIADAESKGLLHPDSVIVEPTSGNTGIALAFVAAVKGYRLILTMPDTMSIERRK
                     LLAMLGAEIVLTDGALGMRGAVEKAEEIVAKTNNAFMPQQFNNPANPEIHRKTTAVEI
                     WNDTNGDVDVFVAGVGTGGTITGVGEVLKAKKPSVKIVAVEPAKSPVLSGGKPGPHKI
                     QGIGAGFIPAVLNQSIIDEIVTVDETDAGTIAKKLAKDEGILAGISAGAAMWAALTIA
                     SRPDSKGKTIVVLLPDSGERYVSTWLFD"
     gene            110931..112208
                     /locus_tag="PLV81_00490"
     CDS             110931..112208
                     /locus_tag="PLV81_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOM08611.1"
                     /translation="MSGNYRIETIALHGGQSIDATKARAVPVYRTSSYVFDSAQHGAD
                     LFALKQKGYIYTRIMNPTQDVLEERVAMLEGGRAALALASGTSAIFYAIINVCRAGDE
                     FISASNLYGGTYTMFDAILPQFGITCKMVPYNDYDAYEKAISPKTRAIFIESIGNPTL
                     GVPDFEKVATIAQKYHVPLIVDNTFATPCHFRPIEYGANVVVHSLTKWLGGHGTAIGG
                     IVVDGANFDWTDTKFALYNEPDAGYHGMRFAHDLGDMNSLAFILRMRLVALRNLGAAI
                     SPDNAWIFLQGLETFPLRMQRHSENALKVAQFLQNHKSVAWVHYPGLVGDSSYKVASR
                     YFNNGFGGMVVFGIKGGYESAVKFINSIKLFSHLANVGDAKSLVLHPASTSHSQLSEE
                     QQREAGITPDLIRLSVGIEHSDDIIAALQEALE"
     gene            112569..113420
                     /locus_tag="PLV81_00495"
     CDS             112569..113420
                     /locus_tag="PLV81_00495"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOM08612.1"
                     /translation="MVKKILIILLCLIVVLGAVYGYVYYKASKVPENNAQAMLKAALP
                     HDKKIVVCFGDSLTHATVSFDYVGFLAKDPDLQTYIFVNEGINSRLVYNLLQVVDTVV
                     ALKPHYVFVWIGTNDLKGSLNDKEYQRYNDLWNLPQKPTKEWFEQNYRQLVSILKTKT
                     DAKIVLISLPPLGENIDSLPFKLSMEYSAIIRDIAQKEKLGYIGLNEILTRDLIREGK
                     RDVAPYTTGLWFMYSAIIQHYLFGRDWDTISDSRGLTYMTDNIHINGKAGRILAAAIK
                     EKLISNK"
     gene            complement(113484..114788)
                     /locus_tag="PLV81_00500"
     CDS             complement(113484..114788)
                     /locus_tag="PLV81_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5982 domain-containing protein"
                     /protein_id="HOM08613.1"
                     /translation="MKKTVVCIIILCLFFALQNAMAARLSDEDLAKKIEGWYPTGLPL
                     VNYDSDNGFGYGVRVYLYNNGTRDDEYFAYAPYKMQLYGQFYQTTNGYQYHEINLDMP
                     YIFGTKFRIITSIAYDKKINANYFGQGADTAKGKLTLYTTYPTAYETFDKFQDYLDYA
                     DNDERFLKYYNYEYTKPSYFLNIYRDITQNLKFLAGLEVKKVTIDTWDGEKFDGTPQG
                     PTLLQQLQPLGYDGGWSNFIRAGIYYDTRDYEPDPKTGYLIDYAFEISDDTIGSDYDF
                     TRHTVQLQYYFPLLQSLTLALRAGYTDANGDTPFFEMGYFGFSLNRRTGLGNNRTLRG
                     YREQRFVGPTMTVANAELRWKFAEANPWGQNFQFKITVFYDVGNVYDKAADPFTDPRF
                     SDYHQGYGGGLVIAWNMATIVHFYYGMSKEDSSVSVDFSHTF"
     gene            complement(114851..115978)
                     /locus_tag="PLV81_00505"
     CDS             complement(114851..115978)
                     /locus_tag="PLV81_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08614.1"
                     /translation="MTKWKIIGFFIFGIIIALTLIIALWFIRPNTSQVDPSLILEVWE
                     AVRDGMHNSNTDMVYFNNYFYLVHASSPYHFATPECKLIVWRSTDCKQWEKLSTIQIP
                     GEDIRDPKFLAIGNKLILFVLKSIEFTAEPYSTAYLISSDGKKWSSLQEIAHKGWLFW
                     RPKTFDGKRWYVPAYWHEHGKAMLFTSTDGTSWEPVSLIYEGDRCDETAIEFLPDATM
                     ISTQRLEYSDSWIGDSRACTGIRIAKPPYTQWAGIKDYSTRLDGPALFAFNGKIYAIG
                     RRNPYTPGLLNKYGSILAKKRTSLWLVTPEKLVWLSDVPSAGDTSYAGYVIQGNTLYF
                     SYYTSDIHHDYPWILGMIKESPIMMGKLDLNVLEQLAYTKQ"
     gene            116234..117409
                     /locus_tag="PLV81_00510"
     CDS             116234..117409
                     /locus_tag="PLV81_00510"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003364394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOM08615.1"
                     /translation="MKDVVIAAIVRTPVGTFGGALKDVTAVELGVVAVKEAIKRAGIT
                     PGQVENVILGNVLQAAQGMNPARQVLIYSGIPQEVPAMTINKVCGSGLRAISLAAQII
                     KAGDADIIVAGGIESMSNAPFALQKARWGYRMNDGVLIDIMIKDGLWDVFNQYHMGIT
                     AENVAAKWGLTREELDEFALGSQQKAEAAIKSGRFKDEIVPVTIKDKTGEKLFDTDEH
                     PRFGTTMEALSKLKPAFKKDGVVTAGNASGINDGAAAAVVMSAQKAKELGITPLCRIA
                     SYASAGVEPAIMGTGPIPASKKALQKAGWNVQDLDLIEANEAFAAQAVVVNKEMGWDV
                     TKVNVNGGAIAIGHPIGASGARILTTLIYEMIKRNAKKGIATLCIGGGQGVAVTVER"
     gene            complement(117514..118638)
                     /locus_tag="PLV81_00515"
     CDS             complement(117514..118638)
                     /locus_tag="PLV81_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HOM08616.1"
                     /translation="MKKQNNNETNPEKVQDSLVQKFKNMVDTVIQPAEHSMRNYVNIN
                     FWRTKYNEYKKIREKEKEDLDIYETLSYGARPTIEYYLLTLLSCLIATAGLLQNSPAV
                     IIGAMIVAPLMTPILAFSLGVIWGDTQLIKTSFSSIIKGSVLAILISGTLAFFVPIAE
                     YTSEILARTKPNLFDIVIAISSGLVGAYGNANKKISNTLVGIAIAVALMPPLCTIGIG
                     VGNFDWHIARGALILFSINLISISLAGAIIFWIMKIHPKSREEKENVTRRALSQIIIS
                     VILLTIIAIPVGIYTYRTFLLEHAKKKVWVTAKKYFGTDVFAIQFDENQGKLDVTVIV
                     LSQQADEESLKQVQNIKSLHPDFGTVNIKFIQTVSSNTTD"
     gene            complement(118810..120189)
                     /locus_tag="PLV81_00520"
     CDS             complement(118810..120189)
                     /locus_tag="PLV81_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOM08617.1"
                     /translation="MDKVSPATRKTALAVTTLGSFLTSFMGSSINVALPIIGKEFAID
                     AIVLSWIATSYLLAAAMFLVPFGRIADIYGRKKIFTYGVILYTFSSFLTAIAQSALIL
                     ILFRVLQGISGAMIFSTGAAMLISVFPPQQRGRVLGINVSAVYIGLSTGPFLGGILTQ
                     HLGWRSIFAISVIVGVAIVLLIYSKLEGEWVEADGETFDFTGSLIYAIALTMVMFGFS
                     SLPSVFGTIMIIGGIAFIFLFIKWELHTRHPVLDMNLFKNNTVFTFSNLAALINYSAT
                     FAVTFIVSLYLQYIKGISPQSAGIILISQPVTMALFSPIAGRMSDKIQPQVIASWGMA
                     FTTIGLLLLTFLHLHTSILFIITCLIFIGFGIALFSSPNTNAVMSSVHKKYYGVASGI
                     LGTMRLTGQAFSMGIVTLILAVHMGRVQITPLYFDRFLQSSQTAFIIFTLLCFGGIFA
                     SLARGKIHR"
     gene            120554..121204
                     /locus_tag="PLV81_00525"
     CDS             120554..121204
                     /locus_tag="PLV81_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOM08618.1"
                     /translation="MAIVPELIKVFIIEDENLVRDTLAQSLELVDNINVVGTSENISS
                     SIGDIKATKPDVLIVDVRLKNENGIEASKNVLKLMPAVKIIILSGYFNDSLAANALAA
                     GASAFLSKSIAISHLVAAIFHVMVHNSFYAPQLPDEVLEDIMVKNKTSKRLCALSKRE
                     KEVLQLIAKEYSTKDIATMLGISEKTVRNHKSNIMAKLGITSHVGLIKYAYYMAMI"
     gene            complement(121258..123885)
                     /locus_tag="PLV81_00530"
     CDS             complement(121258..123885)
                     /locus_tag="PLV81_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOM08619.1"
                     /translation="MNWHTMSVEDAFTFLKTSYKGITQEEAQVRLNAYGFNELKEKKK
                     MTPLMMLLHQFTDFMILVLIAAALLSGLLGELSDTIAIIVIVVLNAIIGFVQEYRAEK
                     AMEALKKMAAPLATVIREGTPVNIPAREIVPGDIVLLEAGKIIPADLRLIEAAQLKIE
                     EAALTGESVPVEKQTQQINEENLPLGDRKNMAYNGTIVTYGRGSGIAVATGMNTELGK
                     IATMLQEEEEVKTPLQKRLAVFGKKLSIAVLVLCAVIFGIGLLRGEEPLRMLLTAISL
                     AVAAIPEALPAVITISLALGARKMVNQNALIRKLPAVETLGSVTYICSDKTGTLTQNK
                     MTVETIFVNNTMFTATQPLQDNRSKSSQLFFYALALNNDAIRDTSGTLLGDPTETALY
                     YYAFTNSFDKDLLTRTFPRVAEIPFDSERKCMTTIHKNTNQEITQSPYIVFTKGAVDT
                     IIDSANTIMIHSEIVPINKDEILKVAEQLSEQGLRVLAVTSKFLDTLPSSITPETVES
                     NLTFLGLVGMIDPPRQEVQEAVSECKSAGIKPVMITGDHPLTAKAIAKRLGIIDENRP
                     YIITGKELQELSLEEFEQKVEYISVYARVAPEQKLKIVKALQDKGQFVAMTGDGVNDA
                     PALKRADIGVAMGITGTDVSKQAAHMILLDDNFATIVKAVREGRRIFDNIRKFIKYTM
                     TSNSGEIWTIFLAPFFGLPIPLLPIHILWINLVTDGLPGLALASEPEEKGIMQRPPRH
                     PQESIFAHGLGAHIIWVGLLMGAVSIITQALSITLWNAHWQTMVFTVLCLSQMGHVLA
                     IRSEKQSLFTLGLFSNKPLLGAFILTFVLQMATIYVPYLNGIFKTHPLTLEELSITLA
                     LSSVIFIAVEIEKFLKRKK"
     gene            complement(124031..126616)
                     /locus_tag="PLV81_00535"
     CDS             complement(124031..126616)
                     /locus_tag="PLV81_00535"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOM08620.1"
                     /translation="MKLSYTFKKRHIVYPVIVVAALYYFIATHPFGTYQAYPDTLSPQ
                     NEGKYTYDIPLDPKSPWPKFRANSLQNGRSAVKPTYRKTLRPWVFKTGKGIFSSPVVD
                     AHGTVYIGSADHYFYAIKQDGSLKWKIKTGEIIDSSALLDDRGFVYVGSGDSYVYCIN
                     RETGFIVWKSKAHSVQEVWEQFTIKTYNVNWFEGNIGMLPDGSIIAPNDNYLVYQIDR
                     QNGKRITQFPANEMIWSLPAVNTKTGKMFFGTQFMALKNVYCYNYRTQKAEWTNGGFG
                     SNAASPLLTNCNVKGMVIVGGYDGYVRAYAQDSGKQLWKQGLRDHIYASPSQTSHGLI
                     IQPCADGTIYALNPENGKIVWAYDTLEPIRSSAAIDGNDIIYVGSGEGKLFCLNPDGT
                     LRWSYLCIDEDRNDLNSSPALGPNGVYIGGENGSVFFIPYDYPLHNAYKDKNCYFGKG
                     EALPNDGAFLVYTSSFGKLLPQPPANIDANQPLAFTLFVRKNGDTLKSSIDRDSLSVS
                     ITGNPKHRIDVSANKQFFVITPQETWVGQDGGTITITIKGQYITGMHRFGLKFFGGSK
                     AGTFHVSYSFAIAPYKGVLPYTYPQITGKPQTVFECKRLAAPNPTMLPSWNQIGFDSL
                     HYVGGVVSKASGGLVVWFIGGKLQDGKTVVDPTLEARYPLYLRSDGKCVTLYNYDGFK
                     INFIGSWDMPFGMYRLSTTANPLTGTIEHSPALVAIALGDEIEFYGRFLKLMGMTEFD
                     TGHMAVFGGLTMSLHGSGVATTPQGAGTISFKAGKTFIKALLKDSRLKKDEHVYSIMV
                     VDAETGNPLPLYYTKRTKVKDINNTVQEISIEFDKGQVKGKVKAYCIVDTYPIAVQLL
                     RIPSE"
     gene            127095..127727
                     /locus_tag="PLV81_00540"
     CDS             127095..127727
                     /locus_tag="PLV81_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOM08621.1"
                     /translation="MEKQRARTGSEKEYRKEQLKKAALTLFAQKGFKNTTVEMITKKA
                     HLSPAAFYLYFNNKIAIYRALNKDGIDILEDLMKQALHTSGGSSVDKLYALARAYYNF
                     YREHTDYFYITEILHLGNDEFFYDSAMVKELEKRTLVLLQIVASVIEEGIEKNELRKI
                     DPMKTAVTLWGMIDGVLILEVKKSTLFTGFTIDALIGQLMDMVLLGVKKK"
     assembly_gap    127947..128046
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <128047..128811
                     /locus_tag="PLV81_00545"
     CDS             <128047..128811
                     /locus_tag="PLV81_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08622.1"
                     /translation="LPFMHHDRQSAVTLLCADYTAAVVPVGSNVWRDTGIEFRGLLAN
                     GLVDYRIGVFDGKDRDASVTPVINKNDMPRFTGRIQVNLKDAEEGFFYSGNYLGKKSI
                     ISFGAGLDYQQDVYNAGTKAKDYTAWTVDVYFDHPVAANNVLTLQGAYVDVKNTPFAS
                     VTVGGTPVYPEKMKMYFAEGGFLINGTWQPVARYYYKKVELSGVDGKISEVAGGLNYY
                     IKGHNANIKVEYAQQLKDDTDDFKNKQITVQCQIFI"
     gene            129308..130369
                     /locus_tag="PLV81_00550"
     CDS             129308..130369
                     /locus_tag="PLV81_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM08623.1"
                     /translation="MFFTKRKYGFTAMLFRVTIACSVVLMLYHENAFARRKDEVLTIP
                     RSEVIKASVDYIGMTDLLATINTLYLNGYYHLMKRHINEYNKKFPPIKEIYYFTAITL
                     INQKKYEDALSRLYIALALEPAYSRALNAIGYVYALRRQWQQALSYFLLAHRANTYNA
                     FISYNIALLYYLNLDYAHAAAWANKAIDDKPNFARGCDVYACSMYQQQKYDEAVEYWG
                     RALQLGLDEDRVYYNRACAYFAMGNYNRCIADCTKALKKNKKNINALILLAFTHFTLN
                     DYDAALSACNNALKIQNNTIVAAVLRAVCAGKKNAQIGKDMLVATLGSVENVQDVAMK
                     LLQNFQYTIIDSPADFMLY"
     gene            complement(130372..130896)
                     /locus_tag="PLV81_00555"
     CDS             complement(130372..130896)
                     /locus_tag="PLV81_00555"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HOM08624.1"
                     /translation="MAQPTIPPKAKLFIGLLTARDDVLFHSINILQKKFGTIDLQSEP
                     IPFSHTDYYSTIGKNLYKVFISFEKLINREDIAKIKLFTNKVETKFLHNGNRTINIDP
                     GYLTLSNVFLASCKDYFHRVYVGKGVYVENEYRYVAKHYQPWEWTYPDYRKKEYLQFF
                     ETLRSIYYEQIKQR"
     gene            complement(130865..131845)
                     /locus_tag="PLV81_00560"
     CDS             complement(130865..131845)
                     /locus_tag="PLV81_00560"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOM08625.1"
                     /translation="MEKDSIISTTTQKLISIVPDGYKPMRLDVYLAGRFTYLSRSRWQ
                     KEIKGGKIFVNNIPITIPHYIVYQHYVIEYKRQPVEEPTVDPHYTILYEDDYIIAVNK
                     SGNLPTHPAGKYFHNTLTMLLWSHTGIQCMPLNRLDRETSGVVVLAKSGRIASAFLQY
                     SKTIAKTYYAIVIGNCSFTRLTVDTPIGISQNSRIRKKRAAYTDAQWHAVTHFTVIKR
                     YNGYTLIKAVPQTGRTHQIRVHCEHIGHPIAGDTLYCNHDDDFLEYIHSKGTIQRPFP
                     RCALHSRMYRLYHPFLQKMIAIKAPLPQDMRTFLLQLESTNGTANNTTQS"
     gene            complement(131952..132413)
                     /locus_tag="PLV81_00565"
     CDS             complement(131952..132413)
                     /locus_tag="PLV81_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08626.1"
                     /translation="MNLETTTCISYEHLDIIQRYAKLHKLSLSMFIINFINYVVSYKT
                     INTKAYSRLSYRTRYSGNWKRLHIVLLEHEYEFIMDVRKVYKMSLAKVIAYCIDNYLY
                     DFLNALEKEDNTDNYRVSGYSFLVFLEEGIQCCQFYWGPHPEILKKANAAP"
     gene            132621..132737
                     /gene="rrf"
                     /locus_tag="PLV81_00570"
     rRNA            132621..132737
                     /gene="rrf"
                     /locus_tag="PLV81_00570"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(132811..133560)
                     /locus_tag="PLV81_00575"
     CDS             complement(132811..133560)
                     /locus_tag="PLV81_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08627.1"
                     /translation="MIYKFFAFIILLFLPQTNLLGYSILPETIHSQKHYLKSTQEITL
                     TSTPAIRAFITQNIPITNNITLEAYYTFKKPQNIKNPMTGSNRLFIINILHSISSMQG
                     IKYYSQTRKRLRILFDKSYIVQYPSLKPLHDPCINKLIDEYSFYAMQKDTTFGEVIYQ
                     YTIYQKPEFILLKSENMDVMKYFMMPIVDKKGFKFFLFILVNGEDIALYCLYSIEVIN
                     DKVFPLQKVRDSLFNRSDAIVLWFYNRLFGK"
     gene            134075..134863
                     /locus_tag="PLV81_00580"
     CDS             134075..134863
                     /locus_tag="PLV81_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017436097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOM08628.1"
                     /translation="MIKVYIVDAFTSEMFKGNPAAVCVLSSVLPDALMQAIAREMNCP
                     ETAFVLPQQDGFLLRWFAPLQEVDLCGHATLAASHVLYEQRIATAQQELRFHTKSGLL
                     SVKKQDEIITMDFPQEIATEYSIPLWVIEALGVKPVYTGKNRMDYLIEVETEKEVLEL
                     NPDFIALKKMDSRGAIITAKSSRKEFDFVSRFFCPGLGVNEDPVTGSAHCCLGPYWQQ
                     KLQKNTFNAYQASQRGGILRVEVGESRVYIGGKAVTVLSGLINI"
     gene            135019..135837
                     /locus_tag="PLV81_00585"
     CDS             135019..135837
                     /locus_tag="PLV81_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOM08629.1"
                     /translation="MPAIIVDKEKCKMDKLCVMECPMKIISVDDSGIPQTLNTAESMC
                     IECGHCVAVCPHGALSLPMLRAEECQDIHYQWNPGVTVIENYFKSRRSIRRFKKDIVK
                     KEDLMKLLEITAYAPTGHNSRTVEYIVYTQKEEIHALVKHVIDWMHTIIINSPEMAKS
                     MHFDMITKAWEDGIDTVTHSAPVIIVAHGKKSNPNTPTSCTIALTHLELIAPALGLGC
                     CWAGYFSWCAMVYHPLKKALQIPDGNTVYGTMLVGYPLVRYYRIPKREPCIEWR"
     gene            complement(135930..136382)
                     /locus_tag="PLV81_00590"
     CDS             complement(135930..136382)
                     /locus_tag="PLV81_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08630.1"
                     /translation="MKKLLLVALIAVVAISFTACGSQKIGGQVKGETFISEGWVTPDI
                     FRVTATGVPKPGLKNKIQRRGTAKEAAIIMAEKRIIEKFVGARLEGAAGAADYASTGV
                     AVAKEFGGLVKGGTIVKETYDDDDNCEIVYQVEKKGLKKEVEGGAYER"
     gene            complement(136610..137146)
                     /locus_tag="PLV81_00595"
     CDS             complement(136610..137146)
                     /locus_tag="PLV81_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08631.1"
                     /translation="MGLLSIMLLCIFYTILIVIVYEYYKLFNSKDEYTKQELKQVVFL
                     IPDHWIPLLSKFRLYPVYALTSLVFGILIPLLSTNWFFQSSVFCILLFIILPQIRRSY
                     EPMKVTVSDSFVDTVATALSEYYEIILFFFSTGTLSSLTYTWVTEKELSFLWFMVNAI
                     IICIIMFFMLASLDKENI"
     assembly_gap    137185..137284
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(137362..137970)
                     /gene="purN"
                     /locus_tag="PLV81_00600"
     CDS             complement(137362..137970)
                     /gene="purN"
                     /locus_tag="PLV81_00600"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOM08632.1"
                     /translation="MKKTVSFLVSGRGSNFQAVASAIIKGDIPATVGLVISDKPGVKA
                     LDIARELSIPAFFVNPKDYPTRQDHEKAMIELLRKYNTDLVVTAGYMRLLTPFFVNEF
                     KNRIINIHPALLPSFPGTHAQKQALEYGVKISGCTTHFIDEGTDSGPIILQKAVPVYD
                     DDTEDTLSARILKEEHQILIESVKLYCMDKLKVKGRKVFIVR"
     gene            complement(138048..139040)
                     /locus_tag="PLV81_00605"
     CDS             complement(138048..139040)
                     /locus_tag="PLV81_00605"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08633.1"
                     /translation="MNKKAVALYSGGLDSLLACAIIQKQGIEVIGLHCILPSVDPAIN
                     PDSLKASQLAKQINLPLVFYRCDDEYANVIAHPRHGYGKNANPCIDCKIFFLQKAKEY
                     MGKLNASFVITGEVVGQRPMSQYKSMLKHIEKECGLEGLLLRPLSAKLLPPTIPELEG
                     IVDRNRLYAIAGRSRDTQFELAQEFGITQFQSPAGGCFLTMPQLASRVKDLIEHPPFN
                     ALDLFLANVGRHYRLHEHAKIIVARNENECTILQRYKQTADFFVEPSFKGPVIYGKGT
                     ILQSDYPVLVQILARYGKPSPDENSVALFIRGHHTTTLVTDNPIDDATLSKMRI"
     gene            complement(139044..140267)
                     /locus_tag="PLV81_00610"
     CDS             complement(139044..140267)
                     /locus_tag="PLV81_00610"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOM08634.1"
                     /translation="MITPDEAIKIILSHIQPLPVEKVHFTNASGRICADDMYATENIP
                     YHTNSAMDGYAIVASDTLNASNNNPVTLPIAGEYQAGIIHNPIPIKSGEAIRIMTGAP
                     IPPGADAVVEKEATTEDNNQVTIFRRVKEGENIRQAGEDIKKGDCIVSRGTKITPAIV
                     GLLASTGNVTVTVAQKPRVAILATGDEIYEPGTPLKEGIVINSNAYTLMSLVQEYGGI
                     PHYVGIARDSMEASKEALTQALSCDIIISSGGVSEGKYDFMISAIAELGFTLLVERIA
                     MKPGKPCIFAHRGNTLYFGLPGNPVSSMVSFIQFVRPAMLAMQGAKALQKPLIKAICQ
                     EPIKRKNDGRTHFIRGIVSFEGGQASVRITGPQGSGILRSMALANCLIILPPDRLSVS
                     QGEHVDVQLIHHPEV"
     gene            complement(140489..142876)
                     /locus_tag="PLV81_00615"
     CDS             complement(140489..142876)
                     /locus_tag="PLV81_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOM08635.1"
                     /translation="MKNLDRLITIISQNLNLDEAFIKNLQHSLSIDEQDKEIITRIQQ
                     LITNAPDQLWDEFHAYLKGINSFKKFFDNFSFIKNHTQALNDYFKRFASGIPDNDYIL
                     NRLKVGIQLEKYDVNPILFSTSYGNVYSYLLEYIKNSINDDSLLAPVVKAYMKIVFLD
                     LALILYAYFFVKDKKLSESKKELAHVTRSYRLIHEINDLIIRTNDEKALFENTVKILD
                     ALGNFDLAWIGIVDNDLKVVPVASCGKTEYLDNIFISVDPAIPEGNGPVGTAIREKTT
                     VRINYPDEDPRYAPWKERAHKYGFKSIISMPIVPDEQHDAIGAITLYSCHENFVSLGE
                     AAMLQEVANDLSFAIRNIRRDKEYEAILFTDTLTGLGNVHYFTSMLSSQMKVASQQNV
                     KLAIVTIDIDNFTAINNSIGYLQGDKILQEFGKRISQLLHNSHLVARSSDEFYTIFPF
                     TDDMELINFIDDLKRLSMTPFDDISNDLMVTYSIGIAVFPGDGNNADDVVHKSRVALS
                     EAKKIGYGTVCYYSEQLFQKNIKKISLQQELRAALKNNEFELYYQPTINLTTRQISGL
                     EALIRWHHPKRGMLAPSHFISVLESSDIIIQAGRWIIAEVSRQVQITPQLQQHGFTVS
                     FNVSPAQFYDSNFIVDVIKSVKSSMINPSHLQIEVTENLLMSDAAEVINTCNALQQFG
                     INIAIDDFGTGYSSLAYLKKLPLSTLKIDSSFVQGLPDNRDDVEIVKTIVLLAQSLGK
                     RTIAEGVETREQLAFLTGLGVDEAQGYYFSKPMPMSDCMEYITNFNHEKYFWPKR"
     gene            complement(143066..143521)
                     /locus_tag="PLV81_00620"
     CDS             complement(143066..143521)
                     /locus_tag="PLV81_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOM08636.1"
                     /translation="MDHEISATTVSKNHLPILKELWEKLNIIHLNDSHYFKDHYETFT
                     FEKRCEKFNVIDDNNIRIETIEDEKTLIGYCIATIQNGNGEIDSLFIEESFRTYGYGS
                     QLLENAVTWLKDNNCTKIMVSVAEGHESVFAFYMKHKFYPRLTYLYLKD"
     gene            complement(143506..143910)
                     /locus_tag="PLV81_00625"
     CDS             complement(143506..143910)
                     /locus_tag="PLV81_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOM08637.1"
                     /translation="MIYFLDSNICVYFLKGLYPSIKKNIQNTNPNNIKIPSIVKAELL
                     YGAEKSQQKAKNLSIINRFLEPFEIIPFDNDCSIIYSKIRSAMELKGTIIGPNDYIIA
                     ATVLAKNGILVTHITKEFGKVKNLKIEDWTMR"
     gene            complement(143907..144134)
                     /locus_tag="PLV81_00630"
     CDS             complement(143907..144134)
                     /locus_tag="PLV81_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004505499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, antitoxin component"
                     /protein_id="HOM08638.1"
                     /translation="MPQISLYIDKETLEKIEKAAAKERISISKWVGKNIKKVIQEEYP
                     EGYFDLFGSITDKSFDIKELSFDQDLKRESL"
     gene            complement(144464..145057)
                     /locus_tag="PLV81_00635"
     CDS             complement(144464..145057)
                     /locus_tag="PLV81_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HOM08639.1"
                     /translation="MTTKTAKHTSNKWYQLKIYLLHIKPLIWRRFIVNSDIKLPDLHK
                     VIQTVMGWTNTHLHQFVKDDIIYSEPDEDSLIEYVDYRKIKLNQLLKKENQYILYEYD
                     FGDGWEHKIVLEKIITEGDFPKHPVCIGGKRNCPPEDCGGPYGYLDLLKIISDPENEE
                     YDEMMEWLGGEFDPEYFDMELINDLLKSKNYGVISFY"
     gene            145672..147666
                     /locus_tag="PLV81_00640"
     CDS             145672..147666
                     /locus_tag="PLV81_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOM08640.1"
                     /translation="MTEHSFMVIIALIIGIFSGFSAIAIRAMIKGFSLVSFPGTQNIL
                     ENIKATPWFKILFIPAIGGLIVGPIIYFFAKEAKGHGVPEVMQAIIKQGGIIRPRVAI
                     IKALASSITIGTGGSVGREGPIVQIGASLGSSIGQFFRVSSTRLKTFVACGAASGIAA
                     AFNAPVAGALFAVEIILGDFGLTQFSPIVISSVMATVISHQFEGNFAAFEVPKYQLKT
                     PYELIFYFMLAFICATVAFLFIKVLYFFEDFFENKVKIPEYFQPAIGGLIIGLMALIL
                     PDIMGVGYDSINAALHGNMIWYFSFILIFFKIIATSITLGSGGSGGIFAPSLFLGAMT
                     GVTFGNLIHYFFPYISATPGAYALVAMGGLVAGATHAPITAIIIVFELTNDYHIILPL
                     MIVCIISTLVTQKLSRESIYTLKLVQRNIFIKDGTDINIMSSIYVNDVYTKNISKIPF
                     YTTFDEVINKLIASHDSYIVVVDKKGAYTGIISMQDIKDNLFDKDVLQSLLIASDIVN
                     KNIPTVNPTHNCQYVLDLLSKHDIEGLPVIDDKSKKILGIIWRKNILDAYNKEIEKRD
                     ITTRFANNIIMPQIDQSVHFLEGYAITEIPAPEMFIGKSIKNLQIRSKYGFEILLIRS
                     NTPHGVKIKAIPSPDYIISINDSLVIAGEIGNINRIKSRI"
     gene            148694..149332
                     /locus_tag="PLV81_00645"
     CDS             148694..149332
                     /locus_tag="PLV81_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08641.1"
                     /translation="MNVNFGLFLRSLVVSILLGFFVSFFVPGSTLLADGGNEPEGYSI
                     NVSHNSDTSFTVEKSYPESAVLKKKYRSQKAAFSYRIDLIVEDAGDDTIIGTHTFTSA
                     IPAGKDSIVIDVKKYVTELKKKIKMRGRVAITLETPSGVRDYVAIGVSDAISVYNSGY
                     QQQFIDNSDLAAQWSEDYNNFIMENQGKDINTINREFLKKYPQYKEFMPYVK"
BASE COUNT        45888 a        25847 c        30696 g        46389 t
ORIGIN      
        1 ccccatccca tgcgctgcca agcaggctgc ctgctcggct taatcctaac catgctaacc
       61 ccagcaggcc ttctttcttt tccatctcca tcatctttcg tgttgcttcg ctgtgttcct
      121 gggctatttt tgatgcccag tcgcttaagt aatccgccat ggtatactgg gtctttgccc
      181 agttttgctc taacagtgca tcgtaaatgc ttttaccctg acccacctgt actcctgcta
      241 aaattatatt ctccagtgtt ccctgtataa acctgtttat tggagctatt gcattagtac
      301 ttcttgccat attcccaagc actgtccctt gtgctaatag ttcgttactt gcccatagtg
      361 ctgcagtact tacacttgct tgtatcaggt catccttcca gcttccagtc ccgtcaaacc
      421 tgttcactaa ataactactt gatgctgtgt acccgtagtg ctgccatgaa ttattccagc
      481 tatcgccata catctgccca atctttgctc ccaccagcgc agtggatgct cctatgccaa
      541 agcttctcca ctgctgcttg cttccccatt gcaagcccaa acccccatcc tgtttatatt
      601 ccacaaaatt acccaggccc tgtattgcac ttgttgttaa tgacccatac actgctgaat
      661 ttgtggctcc acctatatag cctcctgcat agcttgctcc cacacttatc cctgtttgca
      721 ctgcccagtg ttgcaggctc aaatcacctg ttgccacctg actgaattga ttccagctca
      781 tatatgcagc gcctaccatg ggattagctg ttgctatgct cagtgtaact gctatccctg
      841 catcaacata ctggcatata gcatcctctg tagccttttc tttttctgca ttatatactt
      901 cttttgttat acgggcgtat tcgcctttgc ctttgttttt taattttgga cctggttcta
      961 ttactccaaa ttcgccaaca tagtaagtaa aaccccatgg tatatcgggg cgcatcatca
     1021 tgcccatgcg tgtttgcacc tggctcagta ccacattcat ggttgcagta aaagccactc
     1081 catctttttg atacaaatcc atcagcatcg gaatattgta ggtaccaagc accagactgt
     1141 cagtatactg atagggacga tacgcatcta ttttttgctt tttatcgctt actgataatt
     1201 ttcgagtata cgtattgcca tctatcccaa aattataggc acttacaaac tgcaatgctg
     1261 ctgtttgtga gttttcattt gctatatcta tttgttcttt gcaccgcttt aaaagttcag
     1321 ttaacgcttt gagcagtgca atattgtccg tctttttttg agctgtttca aattcttttg
     1381 atagcctgtt tgcatcatca aaaattgtgt aattgtagct tcgcaaacgt atttgcgtca
     1441 agccaaattc agtatgctgc tttgaaagat tcattatttc actgcttaaa agctctggct
     1501 gctctttcag tatatcaaga agcattgcat gcacatccac cttagatatt acatttccat
     1561 atttttcttt catcatggtg accatatcgt atagtagcac ttcaataccc gttaattgtt
     1621 tatgtaactg ttttgcgctt actccgcttt gcgcatcggc aagccattga agctttgcct
     1681 gctgcaattg tttcatacgc tcatcccact gtttattacc ctcaagtatt tgttcctctg
     1741 tttcatcctt ccagtttttc cacctgttca taaaactctg taccatcatc tcccactgct
     1801 tcacacctcg ctggaatatt cggcttacca tgcggtccca gtcggccttt ttatccgcta
     1861 attgctgctt ctttaactgc cactcatgta cctgcaatgc tttccgctgg gcaagctccc
     1921 tgtcatttat cccttcatac tgctttacat actgtgcaaa aagctcttca acctgctgtt
     1981 cattttccgt acttatcatg gcatcaagct ggtttatcac atcaaccggc atgccctcaa
     2041 catagcccat gtatgctagc attgcgcgta tggtgccttt tgactctgct cctattgtaa
     2101 gtgcgctctg ataatcggaa ctatcgcctg cccatattcc gtacaaatcc ttctctacag
     2161 ctctgtctat caccacacga tcatagccac cctgtgtata cattgcctgc acatgcgcca
     2221 tataacggtc aaacacatca cgtttctgct gattcatgat gtttgcatac gtttgcccca
     2281 cggtgcccgc atgcagtacc tgaacggttt catcagtttc atagccattc tcatcagttt
     2341 tgtgtattgt tgtgcgatag ttattgacat taacccaatc cccctgacgg ttctcttcca
     2401 ccataaactg caggtcgcta tcgcttatca ttatgccgta ttttcccgct atctctttga
     2461 gtgccgcttt taacgtgggt ttttgtaccg ataccgtttg catggtcttt gggttgtaat
     2521 atacaaatgc tcctgcttgt tgcatatcat ccagtgactc aatttcagtg tagcgagcta
     2581 acgcagcttt agcctgcaac atcttgagtt taatttcctg gagctcggta agctttgagt
     2641 gtatctttgc aagctgagct tttgcattgt ggtcattgtt tttccatttt tgttcagctt
     2701 ctcgtattgg cttgaataac ttattcaatc ccaattcaaa caatagtact gtgggtttaa
     2761 gaacataggc aagtggacgt aaaggatgta tttttttccc aagtactttg attgttttat
     2821 tccataaata atcaaaacgt ttcttaatgg gtttgtatac ttcataatac ccaccgtata
     2881 ctatgcttaa gttttcataa ctaaactcag catcgcgtgc agttaagaaa ttactatagt
     2941 attcgccgat aagtgcgtta ttggttattt tgttatcctt aagcagttgc agatattcat
     3001 gcatggggct gttggtatct gcaatgacag tgcccccggg aatagagcca aaccatggat
     3061 cggtatctat tttcctgctg gctccaaact tttgtgcatt tttatcaaag taataagctg
     3121 attgttgcag gtgatactca agggaaccaa tagtagctgt tctgtcttta ttgtccttga
     3181 tataatcata taccattttt gccattatgg cttctgatac acctacaggc atataaacac
     3241 tataccctct gctcaaaact aatgctttag caactaacgt tgcagtattc tcatcaatat
     3301 cttttcgctt tacagttgta atgccattta caaaactgta ctgtaacatc ctgtcaatga
     3361 gcttatcacg ctcatcttta tattcatcgt cagttgctgc tataaacgca tccttctttg
     3421 cttcatactg gcctttaagc tcctgatatg caacggaaag ttgcttaatc tcattctgca
     3481 tgcacatggc aagcttttct gttttgtagg ctcgctgtgc tctctcaatt aattcattgc
     3541 gcagcttcac atactcctgg tcatgttcaa ctttttgctg ctgtaccgct tcaacattgt
     3601 gtttgagctc ttctattctt tgctgcacct cattccggta tttcagtgca agttcatact
     3661 gctcttttgc atcagctttt agcttgcttg ccgcatcatc aggatcacca ccctctgcat
     3721 ttgtctggct attgtacaga tatggcgttg atgcatacgc atacagcatc tcctcatcct
     3781 ctttaagctt gcgggcttct tccaatcggt tatacagtat ggaaattatc tcaagctgct
     3841 ttgtgtaatc atcctgtgca tcatggtaag cctgtaaagc attatctacc tgtatttgtg
     3901 cctgctcaaa cagtgttttg ttagcggtgt atacgctctg tgcttcctgg aatgtatcac
     3961 tttcaagata ttcctgtaaa ttgtcacttt cgagtactat ccccatcttt tctatatctg
     4021 catacgcttt cagtagagtc tgctgtatgg tgttgtcttc ttccctcata ctatcaacaa
     4081 gctgcttaat acgtgcatca tctgttccct gtgagtatga ttttataatt tgcagtgcct
     4141 gttctatggg tgatcctgtg ctttcatcgt ctcccatact tacagtgccg tcataccccg
     4201 catcctgcaa ctcattaagc agcccccgta tatcacgcac gaatgatgta tcaattccac
     4261 tgccatcctc atcataaaat gtaaatgtat cggttgcctg tgttgccatc tgtgctacct
     4321 gtaccagcat atcaagggct gctgccatag tggtagcttt atcaatcatt gcattgtaat
     4381 agcttttatt atctcctgta tagtaccctt ttacgttgcc ttccaatccg tcacttttca
     4441 cagcttccat actcccggaa gtattttcct ttctggaaat tccatacccg tttgcatcat
     4501 attcagcaat gaaatcctgt aatgttttat cgccactatc aaatgatccg ttggcattga
     4561 tatctttata ccacttttga gcaatatcca gcccatcctc atcgcgtttc tttaattttt
     4621 ccttatctat aatgacctgg tatagcattg atggtgagtt actatcggca ctgtactgct
     4681 catagtactg ttccatgatt cctatgtaat cacgccagtt gctatacgta ttacgctggg
     4741 aaaggatgaa ttgatacacg ttctgggcta taacactatt ggtatgtgct gcatacatac
     4801 acatggcaag caggctctgc aaaccgttat catcttcatt gcttatagct tcaaatagtt
     4861 cccgtgcttt tgtctgcatc tggtaacctg ttaaaaccag tggaacctgt ccatctgtta
     4921 caataaggtt taccggatcg gctaactcag gagctatact gtacagtatt aaaaaattct
     4981 tttcctttgg atccgtaata ttattttgct gtatatattc ttctatgggc atgctcccat
     5041 cgtaggctaa tgctacatcc attacctgta taaaactttc tgtatgcatt ttaagctcat
     5101 catagccact tatatcagga tgtatgccgt aatatgctaa aagcttttct gcatcatcag
     5161 cgcttgttat cccactgtgt gccattgcaa aataatctgt aaatattgcg tactcacgca
     5221 caccttccgg tagtacactg tatgcaatga ttgagctatc ctgttgcagc ttactatata
     5281 gcatctggaa tgtcggatag ttgttaacaa aaaactgttg ccactcctgc gttccacact
     5341 gtgtcaatgc tgtaaaaccg gaaggttcaa atcctgcttc atgtattgta ccaaatacat
     5401 cctcaaatgc aggcatctgg tcaaaagtac cactcgtaag gtatgccata aaatgagcat
     5461 agatatcagc tggactggta ttgctggcgg tgtatgtata tgcattatgt attgttaatg
     5521 actgcactgt aatgtactga cttactgtgg tattgtaaac ctgcttgaaa tcttcatata
     5581 agcttgtaca caattgcttg atatcttctt gtatctgccc atcagtacta tactgtggaa
     5641 gttttaaaaa ttccccggca cgtaatattt ctccatcaag tagaccaaac acatcatcaa
     5701 aatccgaaaa acctttttca tgtatatagt gctgtataaa tttttgaaaa tgcataagga
     5761 gtgcatatgc tctgaaatcc gtatcataca gcaatgcatt gaatgttgtt gcatcttctg
     5821 aattttcctg tatgccctgg tagtatgctg ccagtatcca gcctgtatca ctttcatccc
     5881 aattattttg cacctcttta atacctgtgg ggttttcttt taacgcctgt atatcctgac
     5941 gttttgtaag ctcgtgtgtt atgtgtgcta ttacatcctg tataaattgc tcatcttttc
     6001 ctgaactgcg catcctgttt gcgtaatcct caacactgct atacatttca ccttctttta
     6061 agtatcgagc aacataatct acaatctcat aatttcttcg tgtttcctct aattttgcaa
     6121 gcttggcaaa tacctgtgcc tgcaaatcgt tatattgtgt gttattaatc tcgtttataa
     6181 cctgggagta taaaacgttg cattcctgta gcgtccgtat tattgcatca gcaaatcctg
     6241 gccacgttcc ccatggctgt agttgatagt agttttgctg tgatgctgta tattcatttt
     6301 cctgatttcc atacatggcg ttgtgtagcg caatgagttc ttcatacttt ttctgtaatg
     6361 aagaaagctt ttcatataca gagcgacttt tttcatacgc tgctgatgcc tggttgctat
     6421 cggtattcct gttttcatac tttgcctttt ggatcaaaac atcatgatac gcacttacaa
     6481 ctgcctgtaa tgcttctttc agtgactggg gcagggtgcc atactctttt gtctgtatat
     6541 agctttctgc tttccttcca tattcaataa gctgtcccac cggcattgat tgtttaaaca
     6601 ttgtttgagc ctgtcctgca tctatgccaa gtatttcagc aatttttccc gtaaaacctt
     6661 catcctgtgt gcgtcttctt ttttctagcg atagttcata gaattgctcc acgtagcgtg
     6721 caaagctttg cataaccacc accgattgtt ccccttcgta tgctaaagga tgaataaaat
     6781 caaatatatc gggttgtgtt actgctgtta gttttacctg tactatatgt aattcggtat
     6841 ctgaaagagg gttgccctgt tgatcttttc ctgtttctaa catgctgata tatgttttta
     6901 tcttttcatc aatggtagta tcatcacatc ctgccagttc atttttatat acatcgatga
     6961 ctatcccaat gtcctctata ctcttttcaa gcattgtggt aacagcatta aatgatgctt
     7021 catcatgaat atctattgaa tcttttaatg ttgttaccag tgtgtaaaga tacccatact
     7081 ctaatgcttc gcgtacattc ctgtttgaaa gagccatatc acacatgctt accagatcct
     7141 ggtaagtgct gttaaaatat tcccagtgaa taccatcact atcggttgct gcttcttttg
     7201 tggcattgag tatatgtaat agtttttcta ctgttttttt gttttgctca aaatatgtta
     7261 cactggcttc atctactgca gcatttgcct gctgtataat gttattttta gtgtataaag
     7321 atgtttcaag gttttcagta tcaaaatcat gaagcaggta cacaacaaca ttgcgagtta
     7381 tgtggtatag atcttcaaat gattttccta aagcattgat tgcatttacc agatcaattc
     7441 ttctaatcct gtattcatcc tgaatctgct tatcatactc agcgtaaagt atctctgtgt
     7501 caggatatac aacctggtta tctacatgtg tggttatatc cagggtatat acatgtttta
     7561 gaagtgcacc aaggaagatt tgtagcgcct tttcatgtat tgtttctgga tctgtctcat
     7621 caagtagctc tgtcagttcc aggctatcta cagtatcagc agtatatgcc accagcacac
     7681 ctattgcagc tttgcgttca tcaacagttg ctgatacatt ggctattgta tctatctgtt
     7741 cctgcagcat actccaggtt agcgcaaagt tgtttgcctg caattcattg agtttactaa
     7801 caatggcttc atgtactctg ctttcagtat tttctatgct gactatcgcc ttccaccgta
     7861 tatataaatc ctttgcttct tcatattctt ccgaatactt ttcgcggttt tcaagggctt
     7921 cctgtaaaaa tgtaagtcgc tcatcttctg cgtaccctgg aagtatagtc tcaagcttta
     7981 gaaataaaat atcattatta tcatcaattg cagccatcac atcatccagt gtaccatttt
     8041 gtatcgtttg ccatgtagtg tacatatcgc gcaacccacg cacctgacct tcatcagttg
     8101 tgtagccctc aagtatcttg gctgttaact ctatgcgctg tgcatactga gaagcttcac
     8161 tattgtgctc ctgtaacatc agtgctttgt aatagttcat tctggctgtt atccacttct
     8221 gctccttttg ctgtatcctg tttgtaattg ctatgatctg ctgtgttgct tcattgacta
     8281 actgtattgc catctctttt gcatcggggt ttgaataata caggcaccgt tccattgctt
     8341 cctgatacaa cttttgagct gcctcatagg cagtgcgtgc tatctcaaat tttttctggg
     8401 catcatctac acgtttctga gctgcaacaa gtgcatcctt aagctgccct gataaaagcg
     8461 ccagtgcatc ctcatactcc ttctttgctt tgttgtatgt atcaatggct gcctggtaat
     8521 ttgctttctg tgtctgtgca tcaggccttc ctgtttcatt gtatgcatat tcatagagtt
     8581 tctgtgcacg gcgaaggcgc tcttcccagt aggcaagagt tagtttttct ttttctaaat
     8641 caaactcagc cgctgtcatc aaatacggat catgggcttg ctggtacaac ccatctataa
     8701 taacagcacc atctgtgtct ttatagaggt aatattcaaa caaaggattg ccctcgttat
     8761 cataacggtc atcggtaagc aatcctgttg tcttctgaac aacctcagta tatccaaaca
     8821 gatcttcaaa atttccagtg gctgttgcag caaactgacg gaatgcttcc atccgtgcta
     8881 tgctccatgc atcagtacct gcaggcccgt tgtactgacc tgtagcggta atctctcctg
     8941 ttgaatatga ataatacagt gaggataaga agggacgcaa tgcttctaac ttttccggtt
     9001 gccctgcata ctgcggaaga ttttttaagc tggcaattag ctcctcaaag gttactatct
     9061 gcccgctacc aagtagaggc aaacactgca tgcctacaat atatttgtgt aaacctgttt
     9121 caaagttaaa tgcattgctt gattgtatat ttgcaatgtt cccttcacgt acagccatat
     9181 ataatgccct gaagttagcc atggcaccgg cgaaggcttg ttcctcaccg gtaaaccgtt
     9241 tcacaaattc tccacttttg aatatggtga caggagttcc ccacatatcc tgagtatggt
     9301 actgcaatgt catgcgggca ataatgtcat tgtaccgtac cttaaggtcg taccactggt
     9361 ttaactgctg aaaaacagct atctttaatt cttttgtttc agttgtgtca gctgatagtt
     9421 tctgtagtat ggtgctgcga gtttgtggat cattgctata ctgcaatcca gtaagtgtag
     9481 taaaatatga accaagccag cttatgtttt ccagggcaat agctaatgat tgagaaccgc
     9541 tcgtgattaa ttcccgtgtg gaggcacaat aatcatcaaa ctgctctttt tgcgctgcta
     9601 ttgtttcatc taaaagtttg agtgcactgg catactgatg atctgtttca ataaaagtgt
     9661 cattccagtt tatgataccc tgccgtatct gctgctgtat gcttgcaagc cactcattgc
     9721 gcttctgtac taattgctca aagctttttg cccagatttc atcagcctgc ctgtacccat
     9781 ctaacgatga gcgttcccat gacagtcgtg ctgctagcaa atcatcctcg gctgctttcc
     9841 atgcatatat tcccgcctgt accagcatat caaggtcatc accttcaggt ggtaagccaa
     9901 tactgctaat ggtgtatgac agcttatttt gaacttctgt taagatggca tctgttttgt
     9961 ctgtagttga ttcaagtatt tgtgcagtaa tctgtgatgc tgattgctgc tgggtgtaaa
    10021 agcgtaaact tgccgtatca ctgtagtgct ggtaaaaata cgtatttttt gcaccgacgt
    10081 aatagagctc aatatctcgc tgtataagct tgtgtatatt gtttagtttt tcatctaatg
    10141 tttcaataaa tcctttacgg tattcctgcg tggtggtatt tatctcagac agcagtgcgt
    10201 cgcgtgtggt ttgtaactgt gagtcaattc ctgctaaaag ctgttgcaca tagtgcccaa
    10261 cattttcatc ccatgcactg tatgttgcca ccatgtcttc atgggggtct tcctgcatca
    10321 tctggtttat gcgtgcatac agctcttctt ttgcatactc aggaacaact atctcaaaac
    10381 agcgtgcgtc atagctgccc agagcttctt caagcatctt ctgtatggca gtgcgggctt
    10441 ctgtcttgag aaaacctgat atgtcatcct ctccggtaac tatcgccccg tcatatgctg
    10501 ttaatgcttc tttttcccat atgctgtatg cggtatctgc agcctgcatg gctttatgta
    10561 tgaaagcttc ttttgtcttc tcagccattg cagattgaag agagcgttca aaatatcctt
    10621 ttgcactgct ctgttcaaat ggaattgtgc cggcaaagcc agttgccaga atgctgaaaa
    10681 aaataataaa cacagtaaca tgtacaaact tcatagacgc acctcgtgtt tacctgaaat
    10741 tggtatatgt acggattttt ccaatggtga tgtttttatg tgtgtatgct gaaaacgtga
    10801 cacagccatg cagtaaagaa cgctgcaagg taacagtgac atgtacatgg gaagaaaact
    10861 tttgttgatg gtatgccccg aattaacagg tgttttaaaa attatggaag gaatacagta
    10921 tatgccacaa gcgtttatat atatgtgcga tagttcatgg taaaggaact cacacggggg
    10981 ataggcctcg cgctggcggt aataaaaacg ctcgtagcag taggcaacag ccagaagcat
    11041 aacgtatacc atgcacagca ttacatttgt gtgtgaatag aaactatgtg taagcacatt
    11101 ccatgcagga gggatgtgag tggctgcagg cgatattcca tcattatgaa gtgaaaaaga
    11161 atatccgcca gcttttatgg tatatgaatg catggcaagc atggcctctt cttcaatgca
    11221 ctgcatagcc ttcattgctg ccatgtactg gctggctttt tgtgttgctt caatgatacg
    11281 cagcctgctt gtagaaggta tatgggtagc ggcagataca tgcacaggtg tggcaaatcc
    11341 acagcacagt attgccagtg caaaagagga tattacgatt tttattgttc ctgccatgct
    11401 gttataatga aaatataaac aagaaaaatg ctttaaatag aatttctata tgtacattaa
    11461 gtatatattt gtactgtaat gttaataaaa ttgaatataa taatattgat tatgtcaata
    11521 aaaaaatctg gtggtaaaac aattttttta ttttatgtag tatttcattc tagaatttgc
    11581 acagtattag aacaggttgt aaaaaaaaaa ttgtttacaa aacaataaaa ataaaatatc
    11641 ttgacatgtt attttcagta ttgtagtatt atctgatgat gcactgttag cattattttg
    11701 gggagttaac tatttatgga tattacaaaa agaactaagg atgatgttgt catactggat
    11761 atatctggtg agatagacct ttacaatgct ccggaaatta aggacatcat caacagtctt
    11821 atagaagaaa aaaaatttaa tgtcatcatt aatctggaaa aagttacgta tattgactct
    11881 tcagggattg gggcattgat atccagcctt tcaaatttaa agaaatatca gggtggatta
    11941 aagatcatta atgtttttgc atcagtgcga aaggtatttg aactcacaaa gttaacgtcc
    12001 ttctttgaaa tatatgattc tgaagaggct gccatagagt catttaaacg gtaagatgct
    12061 agcagattgt gtgtaattac ttctataata tactgaagag tggttcataa aaaatagaaa
    12121 tttttgacac atttgatttt gaaattaatg caggtaaata gttttcataa tgtaatgttt
    12181 ttgggcgata gtaaccgcaa ggaatgttta ttttaatatc aggatttctc ttcctgaaat
    12241 ttaagtatac agattgctac tgatagtagc atcatgaatg taattttgag tcgtacagta
    12301 taatggagat acccaatcaa gtttacataa cagtgggaaa ttttttaagt caagctttta
    12361 catgatagaa cataaactca taatggcagc atgaaggcca tagtgatcaa atgaataaac
    12421 cataaacata atttcggagg aatatatgca cataaacaag aacgtcgctg tactggcagc
    12481 cattgtatgt ttgtcaatga ttactgcctg ttttgaaaag gttcctgtaa aggaactatc
    12541 gcttgcaaaa aatgcaatca ctgaagctga atctgtaaaa gcggattact atgcaaaaga
    12601 tgaatatact gcagctaaag atttgctttt aaaagcccat gataatgtaa agaatgaaga
    12661 gtatgataag gcaaaacaga atgctcaaga atcacagaaa aaggcaaagg aagcctatga
    12721 taaggctttg ccattacttg caaaagatac tctggatatt gcaactcaga gcctgaaggc
    12781 tgctgatgat gcatatgcat cagtacttgc aaaggaagat tatacaaaag cagctgatat
    12841 gctgcaggaa gcaaataagc agtatgaaaa taaaaattac cttgctgcat ataaagctgc
    12901 gttagatgca gatttgtatg ctaaaaacgc ccgcaatgta gcaatgaacc agaaggaaac
    12961 tctggctgat tccataaaag aagtgaatat agtgatagca caggcggaaa gttatggcgc
    13021 agcacagtac gctcctgaga aactggctct tgcaaaagag cactctcaga ttgcccgctc
    13081 tgcgtatgaa gctggtgaac ttaaaaaagg tttttcagca atagaagttg ccaagctcaa
    13141 tgctgatgat gcatacttta cctcacttaa agcaactgct gctaaaaaga ttgaggaagc
    13201 tcaggcagca atagatgcag caaaaggcag cgaggcagct aaactatccc ccaatgatat
    13261 aaaggctgcg gaagaatcgc tggtacaggc aaaatcatta tttaatgatg gcaagtataa
    13321 ggaatcaatg attgcctcag ggcaggctat aaatattgca cagggagtgt cctctaaaaa
    13381 agcagtagct gttgccccga ttgttgagca gcaggggaaa gagggtgtag cggtaaaann
    13441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga agaagaaact gaatatacac
    13561 tgtataaagt taagtataat cctgcaaaaa gagattgcct gtggcggatt gctgaaaagt
    13621 tttacaatga tggattcaaa tggaaagtaa tctatgatgc taaccgtgat atagtaaaaa
    13681 atcctgattt aataaagccg ggctggatat tgaagatacc aaaagctgca acaaagctca
    13741 ctcagcctgt aaatcccgta aaagagaaaa atactgatgc aattaaagag aatgaaccgc
    13801 cagcaaccga acttaagatg aagaccgaat aatgtatata caaggggctg cgctagcagc
    13861 cccttgttgt tattcccctt ttgattcttc ccatagttca tccagctcct gcaatgatgt
    13921 ttgtgttata tccttattca tttcatgcag tcttttttct atgtgctgaa agcgcgcaat
    13981 aaactttgag acggtatgtt gcagggcttc ttcgggattt atttccatat gacgcgcaat
    14041 attgacaatg gtaaatagta tatcaccaat ttcttctttg agtgcttccc ggctgtcttt
    14101 gttattttgc aatgcatctt tgaattcatt tacttcttca tgtaacttga caactatatc
    14161 ctgaatatgt tcccagtcaa aaccaacacg tgataccttt tgctgcacac ggtaagcttt
    14221 cagaagtgcc ggcagatgta ccggtactcc attcaacata gactggccat cttccttttc
    14281 tttctttttt atcttttccc agcgatcaat tacctcgtat ttatcttttg cggtttcatt
    14341 gccaaataca tgggggtgac ggcggattaa tttttcaata ataccactgg caacatcatc
    14401 aatatcaaat atattctgtt cacttgcaag ctgagcgtgt gcatagatct gatataacag
    14461 gtcgcctaac tcttctttaa gattttcggt gtttccgttt tctattgcct gatacacttc
    14521 atatgcctct tctataagat aaggtttgag tgtatctgac gtttgtgctt tatcccacgc
    14581 acatccgtgt tcactgcgca atatactggc aatttctttt aatttataca ggggtggtat
    14641 tgacatgggt tttctccaga taatgaatgt gcattatgta atgatgtatt gagtgtatag
    14701 attactaaag agcaaatggt atatagtata taataattca atggtttcaa ttaacgtaaa
    14761 agtaatccaa aaaaaattac atactacgat tatatcgcgt agtatacatt tcatcaatca
    14821 tctttgacgt gaattgataa tactcgctca tttcgataca attttgccat aaaatctggc
    14881 aaaattactc aatgagcgtt gtgaaatttg tatcgaaacg acttttggga cagcccctta
    14941 caaaaataaa cgaataaaaa ttttatgttg acactattat gtaattgtgg acatacgcag
    15001 tatatgcgtg tatactacta tacatacgca taaccatttc tcccgtaaca agaggtaatt
    15061 catgggttta caaaaatatt taaaagattc agatcctgaa gtcttttcaa tcattcagaa
    15121 agaaaaccag cgacaggaac attcgcttat tctcattgca tctgaaaact atacgtcgca
    15181 ggcagtgctg gaagcatcgt cctgcactat gaccaataaa tatgcagaag ggtatcccgg
    15241 caagcgctat tataatggct gccagtttgt tgaccaggtg gaaaccgtag ctatcgaacg
    15301 tataaaaaag ctttttggtg ctgatgttgc aaatgtacag ccacacagtg gaacacaggc
    15361 aaacatggca gtgctgatgg cgttacttga accgggcgat accattatgg ggatgaacct
    15421 gtcacatggc gggcatcttt cccatggctc gccggttaac ttttccggta tttattttaa
    15481 ggttgtttcg tatggtgtca cgaaagacac gcatcgtatt gactatgatg aagcatatag
    15541 attggcaaaa gaacacaagc caaaaattat cattgccgga gcatcggcat atccgcgcat
    15601 cattgactgg aacagattca gggagatagc cgacagcatt ggcgcggtgc tgttaacaga
    15661 tattgcacat attgcaggac ttgttgcaac tggacaccat ccttcgccgg ttccggtagc
    15721 cgattttgtc accatgacaa cacacaaaac gctacgtggg ccacggggag gtcttatttt
    15781 agcaaaacaa aaatatgaag gtatgatgaa caagtatatc ttccccggca ttcagggtgg
    15841 gccgcttatg aataccattg cagccaaggc agtggcattt ggtgaggcat tgcagccatc
    15901 attcaaggag tattcggcaa aagttattgc caatgcaaaa gtgctggccg atacattatt
    15961 atcacgtggg cttacccttg taagtggcgg caccgataat cacctgatgc tggttgacct
    16021 gcgaaataaa aatttaacgg gcagagacgt tgctaacagg ctggatgaag ctggtataac
    16081 ctgtaacaag aacggcgttc cctttgacga taaatcgccc atggtaacca gcggcatacg
    16141 tttaggaagc cctgctctga caacccgggg tcttggtgaa gatgaattca ggcttatagg
    16201 cactttaatc tgtgatatta ttgacaacat ggatgatgaa aaggttctta atcgggtgcg
    16261 ctcacaggtg caggagttat gcaaagccta tccaactgat tttttgcgtc tggagcccag
    16321 gggaatatag tgcttgactg tgcaggtcat gtgtagtatg tttcatgtat tgtaagattt
    16381 caagagaggt tcagaatact aaaaaagggt tttatcctta aatgtaattc tggatggctt
    16441 tatatcaata gattccagat gaggtttggg aaaatttttg atttcactat attcactgat
    16501 aaataagtaa tactattttt ggaggagtat atgaaaaaac tagtagcagt tatcttgatg
    16561 tgttccattg cagtggttgg ttgtaaaaaa gaaaagaaat ttactgtagc ccgagaagga
    16621 ctggttaact ttgttgccgg tacggtactt cttgtttcag gtggtgctga aagcaaggcc
    16681 cagattggcg atgcaataaa agaaggcatg accattaaaa ccgttgggcc aaaatccatg
    16741 gctgaaattt attttggtga aaatgcggta aagattttag gtgatactgt tatcaaagtt
    16801 gaaaagctca ttaccaatct agactctaac actgaagagt cttcgtttat cgttgaaaaa
    16861 ggacgtatgt tctcaaaggt aacaaagaag ttggccaagg gcgatagtta ccaggtaaaa
    16921 accccaacgg caatagcagc tgttcgtggt accgattttc tggtaacaca ggaagaagac
    16981 aagagcaatg ttgcctgttt agacggcttg cttgaagtgt tgaaccagag tttagctacg
    17041 ggccagccta tagtactgga agaaaagcag gaagttgatg ttgttccggg acaggatatg
    17101 gttgcaaagc agatatcaga agataagctg cgcatgctca atatcttgct tgaaattaag
    17161 gcaatgcgcg aggatatacg caagaaatat gagcagcaac gtgaagagat actgaaatat
    17221 gttgaagaac agaaaaaagc tgacaaaaca atgctagaag agcagcgcga aaaggataaa
    17281 gcactcgttg aagagcagaa agcccgtgac aaggcaatga tagaaggcat taagggtgag
    17341 ataaaggatg aatcgggcaa agcagctcag cagtcacttg atgcggcaaa gagccagatg
    17401 caggatgcaa aagctgttga taaggatcag gcaagcactg aagccaagga tcagatgagt
    17461 gcaatgaagc caaaaattga aaagccaaaa gtagatttga atcagttcaa gacacagtaa
    17521 taactgtaat ataagataga taatggggct attatcacac gtggtaatag ccctacctgt
    17581 ttaccggtac gtgaatatat gtttaaagtt tcacagggaa aagagaattt tagggtaaaa
    17641 agaatagaga gtgatttcct gtagtaaaaa gtttaaagaa ataattgtta agataaagta
    17701 ataataatta ttgttaaacc cagcaagatt gtataaaaat ggagtaatgg gcaaagacgt
    17761 gccgtacagg gaagtacggc acgttgggtt gcgtgcgagg acctgaaggg atgctattcc
    17821 gcagcacgca gtggaaggat tgatgaatag caagtaaggc acaccgccgc atgaggtgcg
    17881 ctttgggggt ttgtaagggg gcatttagcg cataaatgcc cccttataat gattcatttg
    17941 atgcttcatt gcaaattgtg atggaatatg agtgtaaatc attgccagca aaaaatattg
    18001 tttgcatttt tattgcctta ccactaccct gtggttaatt tgaagcttac gtatctggta
    18061 ggtgtatggt taagttagcc tttttaggaa cagggacctg taacgccaca caacgcaagc
    18121 cacaggcact tgctttttcc atagatggtg aactggtatg tgttgatttt ggtggtggtg
    18181 cgtatcatgc catagcttcc cttaatgatt cggatttcaa ctacagcaat atttctacag
    18241 tgatacttac ccattatcat gttgatcatg tttcaggctt gcccgatctt ttatggggcg
    18301 agatgtggga ccatacaggc cacagaactg aaacgttaac cattgtgggt ccaaaggggt
    18361 tgcataattt ttaccatgac aggcttttgc catttatggg tgactaccca ttgccgttta
    18421 aggttgaatt gattgaatta aataatgggg atgcttttga aggtaatgga tacagggttg
    18481 aagctgtttc tctggcacat ggcgactttt cgtcaggata ccgctttgat tttggtacac
    18541 ggtcagtggc aattaccggt gataccggtt actgcgatgc attacagggg ctactttcat
    18601 cagcagatat tgcggtaata gaatggggaa tagcttcgga tgatacctat ccgttgcatc
    18661 tttcttcaaa agatataatt gcattaatta aaagtggtgc ctggccacgt gaggtgtatg
    18721 cggttcatgt atatccagtg gtagatatac attttcagga gcagctacaa aaaatgaaat
    18781 cactggttgc ttcccttaat aaaaccattg aatttccttc cgatggcgat atacttacaa
    18841 taattccatg atttttatat gaagtaattg aaatgtacta tactcctgac cttctggtag
    18901 taaggtgatg taaaacgtat taataccact cagattataa tgagaggatt aacgtctgat
    18961 gaagaaaatt gaaactgaat actgtattat tggcgcaggt gttgttggcc tggcagtggc
    19021 gcgggaacta tcgcttaaaa aaaagaaaaa aataattgtt tgtgaaaaag aagagcatgt
    19081 cgggatgcat gcttcaggcc gtaacagtgg agtactccat gcaggcattt actatacagc
    19141 cggaagcatg aaagccagta cctgtattga aggcaacagg ctcatgaagt cattctgcaa
    19201 gtcaaaaggt gttccggtac gtgaaactgg taaagtaatt gttgcaaaga acagtaacga
    19261 actgcaaacg ctctccatgc ttgaacagcg tgcaaaacat aatggtgttg aagtgaagtt
    19321 gatcaatgca aaaaaactat ctgacattga gccctgcgca aaaacatatc aggaggcatt
    19381 attttcgcca cacacagcgg tggtggatcc aaaagtggtg atgcaggcat tatatgaaga
    19441 ttgtacacaa agaggtgtga acgtattgtt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19561 nnnnnnnnnn aatatagtat taaaagatgc attgctgttt tttgctaaca gaagcttcag
    19621 gagtgttgcc attgaagaac caaaaaaata tatcccctat tatttttacc gtgatgcaaa
    19681 agagctggtc tggaagctat tgtacagcga tatagaaaaa acaacaaagg cgggcatacg
    19741 gccgcagtta gttgattggg caaaaaaaga gctggtaatg gatttttgca tctatcatga
    19801 tgcacatacg gtacacgtgc tcaatgcaat atcaccggca tttaccagtt cattatactt
    19861 tgcacaatta attgttgata gatattgtat gtaactgcat taatgttttt tattggagat
    19921 agtttttgta atatactata ttgtatataa aatattcaac taacataaag gagtattaat
    19981 atgaaaataa gcgttgtggg aacagggtat gttggtctgg taaccggaac ttgttttgca
    20041 gtaatgggaa atacggtaat ttgtgtggat actgacagtg ataaaattga aaaacttaaa
    20101 aaaggaatta tcccaattta tgagccaggg cttgatgagc tggttgtaga aaatcataaa
    20161 aaaggaaatc tactttttac tactgatatt aaagaagcac tggaaaaaac aaatgttatg
    20221 ttcattgctg ttggcacgcc catgggtgag gatggcagtg ctgatttgcg ccatgtgctt
    20281 gatgtagcac agtcaattgg taaatttatg acccatgata tgattgttgt ggataaatca
    20341 acagtcccgg ttggcactgc tgaaaaagtt aaagcaacca ttcaggaaga actgaaaaag
    20401 cgtggtgtta ctttcaaggt tatggtagtg agcaatccgg agtttttgaa ggaaggtgct
    20461 gcggttgaag attttatgcg tcctgacagg gttatcatag gaactgatga tgaagaagca
    20521 ctgaatgtta tgaaagaatt atatgcacca tttacacaca gccatgagcg ctttatagca
    20581 atggatatac gaagcgcaga aatgacaaag tatgctgcaa acgccatgct ggcaacaaag
    20641 ataagcttta tgaatgaaat agccaatatt tgtgaaaaag taggtgcaga cgttaatatg
    20701 gtgcgcattg gcattggcag tgatcataga attggctaca gcttcattta tcccggtgtt
    20761 gggtatggtg gttcatgctt ccccaaggat gtgcaggcgc tgataaagac ttcaagggat
    20821 tatggatatg aaccaaagat aatcactgca gttgaggaag taaatgcaaa acagaaactt
    20881 tcactggtgg ataaggttgt aaaacgcttt ggccaggatc tgtcaggtaa ggtttttgca
    20941 atctgggggc ttgctttcaa gccggaaact gatgatatgc gtgaagcagc aagtatcacc
    21001 attataaaag agttattaaa aagaaatgca aaagttaaag cctatgatcc caaggcaaca
    21061 gaacaggcac aaggatttta tcttaaagga ttaccgaaca ttgagtattg cgatagtaaa
    21121 tatgatgcac tcactggtgc ggatgcgttg ctgctggtta ctgagtggaa ggaattcaga
    21181 agccccgatt ttgatgaggt caaaaagcga ttgaaaaatc cggttatctt tgatggcaga
    21241 aaccagtata atatgaaaaa attgcaaaaa gatggcttcg aaatttatca aattggtgtg
    21301 ggcaaagtga aataggttat atgattaagg taccatcacg cagagtatac agggagagga
    21361 tagctttatc tcccttgtgg tattcaatca atactctgcg tgaactatcg tactattaaa
    21421 aataatatcg tgctcctaag cctccgcgaa tatcaaaatc aacatcggga ataatacgta
    21481 gtgcaggtac aagttcaata aatatgccaa ctggaacttt tgtaaatgta tattcaagcc
    21541 ctaccgggat acgaacatca attctgtttt cttcatcatg gtcatttttc ctgtcttttt
    21601 cccaatcatg gaatcctgca ccaacaccaa ggtaaaacac cagttcgtta attgggaaaa
    21661 caccatggaa atggcgtaaa taatctgcat aaatatataa atcatctgca gattctccta
    21721 tgccaatatc tatggcatca cttttcccca taaaaatttt tgctgttaac cctgatgggt
    21781 cacccagaat gaagccagca ccaaagttat gtgcagcaga tgctatagtt gcaaacatca
    21841 atacaaaaat gcatggcaac aaaatgtttt tcatgggttc acctcaatgg cattgataag
    21901 gttatgtaag gtaaaattat tatacagtat aattcaacag gtacgtcaat tcaatttttt
    21961 aatcatactg cgtgtccata aaatttttag tatgattcat tatatgttat ataatactat
    22021 tcatagtagg tgagctaaac acactttatt attaagatgt aaaaagagta tgacaacacg
    22081 acaacgaata ctgagaaaac ttgttaacct ttatccacca tttttaggtg ccggtatacg
    22141 aataaaattt gatgacacaa caaataaggt tgcggtttca atgaaactta catggtataa
    22201 ccgtaattac gtggggaccc attttggtgg ctcgctgtat accatgtgcg acccgtttta
    22261 tatgatttta ttgatggata tccttgggaa agagtatatt gtatgggata agtcagcaac
    22321 aattgaattt gtaaaacccg ggcgctcaac agtccatgct gaattttata tacctgatga
    22381 aattgtagaa aagataaaaa aggaagttga taaaaacggt aagtctatgc cagaatttgt
    22441 tgccgaagtt aaagatgaac agggtgaggt ggttgctcgt gtgtataaat atatttatgt
    22501 caggaaaaag gataccggta gataaatgat atatcctgca accgaaatat atatccagag
    22561 ggcagcacag gcaattaagc agggcaatgt agtggcattc ccaactgaaa cggtatatgg
    22621 tcttggtgca aatgctttaa atgcagaagc agtggcaaag atttttgaaa ttaagcaaag
    22681 accttatttt gatcctctta ttgttcatgt ggattcgcgc gagatggtgc aggcggtggt
    22741 agctactcta ccggatattg ccctgcgatg tatggaagca ttctggcctg gtccattgac
    22801 actggtactg ccaaaaagca caataatacc ggagatagtt actgcaggac ttgataccgt
    22861 tgctgtaaga atgccacaac atcaggttgc acaaaaactg atacaggcaa gtggcgtgcc
    22921 catagctgcg ccaagtgcaa acccatttgg atatttgagc cccactactg cgaatcatgt
    22981 tgcatcgcag ctgggtgata aggttgaaat cattctggat ggtggacagt gccctgttgg
    23041 ggttgaatca accattatca agatagaaaa agatagcatt acattgcttc gtcatggggg
    23101 gatacccgtt gaagatattg aagctgttgc aggtaaaata tctgttatac gtcacacaca
    23161 ggttgaagct cctggtcaat tgccctatca ctatgctcca aaaacaaaaa ttgtcctgat
    23221 aaacgatata agcaaaatcc ctgatgacag ttcctgtgga gttattcttt ataaaaaaag
    23281 ggatctgcca tttaaaaata tccatgtcat gtatctttct gataatggtg atttgcggca
    23341 ggctgcagca aatcttttta catgccttca tgctcttgat gcaatgaggc tttctgtcat
    23401 atatgcggag gcaattcctg aggttggtct ggggcaggcc atcatggata gattaaaaaa
    23461 agcgcaagca cgtcacagct gattacgtat aacattcctt ttaaaagcaa cattcttttt
    23521 tatcagtaac tatcgccaat aatatggttg acatgagatg attttgtagt atataaaaag
    23581 tatacttttt acaggagtgg ttgcatgatt cagtttgatt ttaattattt tatggatgag
    23641 tttattggat ccgagcatgg tattgcaaag aaggatatag atgatcttac gccacaatgt
    23701 gatgctgcac gtaagcgaat ggatgatgaa aaggcaaagg ggatgctggg ttttctggaa
    23761 ttgccatttg ctgatactat tgttgaggat atacgtgcat ttaaagcaac agtgccctgg
    23821 tgtgataccg tagctttaat tggtattggt ggatcagcgt tggggccaca ggcattgaaa
    23881 aatgccctgg cggatattta tcttaatgaa ttgcctgatg aaatcagggg taaacgatgt
    23941 aaagcatatt tttttgataa tgttgatcct ggtgagatgc aatcgttact aaaaatactc
    24001 aatattcctt caacgttgtt tttggtcata tcaaaatcgg gagggactac tgaaaccaat
    24061 gcaaactttg caattttact ggatgcaata aaaaaacaaa ccaaggacta caaaaaacac
    24121 atagtgacta taaccgatcc cgacaaaggc atattacgac agataacaca ggaagagggg
    24181 tttgtgtcat ttcctgttcc atcaaatgtt ggtggaagat tttcggtgct ttcagcggtg
    24241 ggcctggtga ttgctgaatt tctgggaatt gatgcgtatg ggttgctcaa aggtgctgca
    24301 cgtgttgtag atcgttatta tggtaagact gtgtgggaaa acgctccgct attaaatgca
    24361 gcatttcatt acctatttga tataaaaaag aataaacgta ttaatgtgct catgccctat
    24421 acgcgcaagc tgtacctgtt tgctgactgg tatcgtcagt tatgggcgga gtcgttggga
    24481 aagcgttttg atatacaggg aaaagaggtg atggcaggcc taacgccggt tgccgcttta
    24541 ggtactattg atcagcattc gcagctacag ctttatctgg aagggccaaa tgacaaagtt
    24601 ataacatttc ttaaactgaa ggattttgga accgacatac tgatgccgca gttttacaaa
    24661 ggcaaacagg aactggaata ccttagcggg aaaagtttgc ggaagctgaa tgaatttgaa
    24721 gaacaggcaa ctgagcttgt tttgaaagat gctagccggc ctaatgcatc gatcatactc
    24781 cccaaacttg atgaagaggc tgttggtcag ttaatcatgt ttctggaaat gcaaacagca
    24841 tacgctggtt atctgtataa cataaatcca tacgatcaac ctgcggtgga acaggggaaa
    24901 cgttttacgt ttgggctgct ggaacgaccg gggtatgagg actataaaga aaagtttgta
    24961 aaagggtatg taaaaaaaga atcatatata ctataaaaaa atcacaatca tgaaaattaa
    25021 aaccccgctt tttattgtct ttgaaggaat agatggtgca ggcaaatcaa cgcaggccaa
    25081 tcttttgttt tctttttgca gggcaaaaac tcccgcggtt cttttgcagg agccaaccga
    25141 cagccagtat ggcaagcttc ttaggaaaat gctaaaagga gaaatccctg gtacaaggga
    25201 tgagcttctt gatcttttta taaaggatag ggcatatgat gtggaacata acataacgcc
    25261 atcgcttaac agagggtatg tggtgatagt tgatagatat ttttattcaa ctgctgccta
    25321 tcaggcaggt gatggtatca aatcttcaga cattgtcagg atgaatattg aaagaggatt
    25381 tcctgtgcct gatagggtgt atttcattga tattgagcct gctatcgcct tagagcgaat
    25441 acacaggcgg tcaggtgaca ataaagaaat atttgaaaca ttgcaaactc ttgaaactat
    25501 cagaaaaaat tatttgtcaa tagcagactc caccttcgct gtcatacagg gcagtctggg
    25561 agtggaagat atgtttcagg aggttttgaa ggattttgaa aaacattttg tgacaggata
    25621 gatagatagt atatgaggct atttcgccag ttaattattc aggggattaa tattattttt
    25681 ctttcccttt tgctgctttt ggggtggatg ctacacactg tgaagattga aggtaatctt
    25741 cttgtaatat ttatagtttt ttccatcgta accatatttc ctctgtatac gtattatatt
    25801 gaacgatttt ataaaaacat aattccatta aaatatgaag atctctatat acagacagtt
    25861 gacatgtttt tgtcgttgca gtcatttgat gatattatca aggttatctt tgataaggta
    25921 ttacagtata ttggtgttaa gtcggggctg ctaatatttt attcccatgc aagcgatgat
    25981 tacaccattt actatcagaa acagcaaaaa cagaaagtaa tacgtaaagc ccatattgat
    26041 aaaaataata ttatattcaa gataattcaa tcaggggatg atattctgat aaaaagcaaa
    26101 atggatcctt cattgcattt tcaaagaagc atcatgtttg aaatggaaaa attgggtgga
    26161 gaaattatca tccccattta ctatcatgat atttttttag gcctgatgat cattggtgaa
    26221 atgaaacgaa gaatatcccg gagggatatt ttaatgttaa aagcattagc ttcaaaaatt
    26281 gccacagtta ctgttaacag cttctttatg catgaactga tcaaaaacaa agagattgag
    26341 aaggaatatg aactgggaca taaggtgtta aagcaatttt taccccctga taaaggtatt
    26401 attcacaaga ctcaatatat gatgatacaa aatccggaca gtaccattac ctgttatttc
    26461 aatatttaca atccggatcc aatgacctcc tatattgcac tatgtccgtt acaggccaat
    26521 atttcactgt tatcattgtt gattccggca atcagtgtgt taatgcagag ttattcccgt
    26581 cttggatttt tacctgatca tattataaag aaaataaatg gtgttcttaa agaaaaagaa
    26641 ctcattgaag aaaatttgcc catgatgatt gtgaagaaga aaaagaaaca atattgcata
    26701 agcaggtccg gaaactgtaa tatgaaggta tacgttcaga aaaaaactaa agttatgccc
    26761 ttgaacctgc aagatgctat taaccaggtt aataatatca aaaagcttat aataacatca
    26821 ggatggcttc ccaataatat tggaggaata gatgaagttg atgaaaccat tgatgtggac
    26881 atccacagta taccagagaa caattttatg ttaatacttt cagaaatact atgaaaaaac
    26941 ggatactttt ttacagtttc agtattatat atactctgat ctttgtgctg actgttcctt
    27001 atggctataa gaatgcagtt acatacccat ttgaccggct gctggtaggt agtacgtttt
    27061 tgtatcgttt gcatgataca ccacatgctt tatttaaagc tacacttgaa acatgtgata
    27121 gtagaactct gaataatgat ttccaaacgc taactgattg ggaatgtata tctaccatta
    27181 ataatacaac tgttaaacaa cattttaaaa gtaataataa ggtagcattg tggctggttt
    27241 taatactagc attttcttca attcttttga ttgtatgggc tgcgggcgtc tattatgcac
    27301 ggaaggacat gtttgctaat ttatttatat tgattgctaa tgctgtggct cttaattgtc
    27361 ttgtatacat agaccttttt tttgtgcatc ggttacatgc tgtctatcct gctgtcagct
    27421 ctcttgttcc cctctgttta ttctatattg tatttacgat gaaagaaagc aatcagctag
    27481 ttaaaacagt gtgctatgtg attgttcttg taaccggcat tgttttaagt ggaaccgtct
    27541 attgtaatgt tttggccttt gaaaaagtgt atcagctcaa tgggattatt tcagttgtat
    27601 tgtttattat agctgctatc atgttggttt gtgagcacaa ccatgcacga tttttaatta
    27661 aaagaaatat tgtagtggca ggcgcaatag tagcaggtgg tgttttgcca tttgcagtgt
    27721 tgcttgcagg tacgtatcat gatatgcagc cttctacttt attttcactc ctgtttacca
    27781 tagctatacc ggtgagtata gggaatgggc ttctggcaaa ttacagcctg gggtcggtgg
    27841 ccaatcttca aaaaaacagt atatcaatgg ttgtggatat tattatcgca gtggttatat
    27901 cgacggggtt attttatatg tataattttt attccatatt actatcgccc cccattatta
    27961 ttggactttt tcttgcaatc atgctgatta tcctccacat caggatgctc atcatacggg
    28021 cgcttgatga tattatattt ttaaaaaacg atgaatattc tctgtcatta cagagaattg
    28081 cagaaatcat aacctggcca aaaaatttaa aggaaaaact ggatgctatt gaaaatgaac
    28141 tgaagacgct aatcaaagtt aaaaaaataa gctatgcaat tattgataac tatcagtata
    28201 atgatgaacg aattattgtt ctggatgaag attctaatct tgcctcgttt tttaaaaaaa
    28261 ggaatgaaac cattacctcg gcaagttttt tttccagcag aaatgatgaa aaggctgttg
    28321 aagaatacat tataacccac ggtattgaag cctgcattcc cgtatgtata aatgaatcct
    28381 taaagggtat tctttttgtc cataacaaag gcgattattt tacaaatgta gaactaaact
    28441 ttctttccat gttagccttg caggttcatc agcttgtcat caacagcaag gctttaacgg
    28501 aatatatcaa ttcacgaaat tatgagaagg aacttgatct ggcatcatat attcaggttc
    28561 gacttttccc gaagaaacca ccacaggatt gtggtattca gttttctata tattcacggc
    28621 cgtatttgaa ggtaaccggt gactactatg atatcatccc tgtggacaaa aacagggtgg
    28681 cagtagttat tgccgacata tcaggccatg ggttatctgc tgcaatgata ttatcagcca
    28741 ccagcgctat tatcaacggg atgttaaaag aaaagaaagg tattgataaa gtagttgctg
    28801 aattaaatca ttttttaact atccgctata cgggatttga actaataacg ttatttatag
    28861 gcctgtataa taagcggaca cgatctatgg aatacatcaa tgcagggcac attgcaccac
    28921 tggtagtttc aaaggataaa aaattatatc atctggaagg aagatccaaa atactgggtg
    28981 ttgacccaat ggcacagtat tattcttcgc gctattcatt cagcgcaggt gatcagatga
    29041 tactgtatac agatggattg acagatctgt ataacagtaa tagtgatata acatttggtg
    29101 aggattcatt gaatgaaata ctcaatcagt cattaacaaa acctattgag gagaaaatcc
    29161 atgcaattac agaagggatt gttacatttg gccaggaata tatcaaggat gacattactg
    29221 ttattggcat gcattttgat taatgctata attaccggga atgcctttcc tgatgtaaag
    29281 cagcaagcac cagatgtatt gatgccaggt gctacggtat caatgccagg gataccatcg
    29341 tatagttata taaatccagt ttttgctgat attaatgaag ccttttcatt acagtaccgt
    29401 tatgcacaca ttaattccgg tgatatagga acgcattatg ccgctacaac attatacgga
    29461 tttaccattg catatagtac agtggaagca ttatttgctg aaaaagaagt tatacaaaca
    29521 cagggggaat catggtattt tgccaaaggt attatgctca ctgactggtt gggtgttgga
    29581 gtaagttata accggtatac agtgaacaat gaccctgtaa catcatgggg atacagtttg
    29641 cttttgatgc catcacggtt catttcattt ggtatggtca ttgatcatgc taataaacca
    29701 tcgttagatg aggttacatt aaaacagcgt gaatggtatt ctattgggat tcgtcccttt
    29761 ggtaatactc tttcatttgc atgggacatt aaacgctatg agggtgaacc gtggaaagat
    29821 gtattgcatg tgtttaccct ttcaggaatg gtgtggcgcg atatcatatg ctctttgcag
    29881 tatgacatgg atcgcaatat atcatttgca ctggctatcc catttgacat atcatcacga
    29941 actggtggta gttctctcta tgatgttggg tatatgcatt ttgataagca gaaggcaaca
    30001 gcctatacct ttggtatcac actaactgaa gagcgcttca ggaatgcatt gagttccaag
    30061 atgcggtatt tgtcgatagt cctgcgggat caggttaaag aggttaagga caaaagctta
    30121 tttgagccat caaaagcatc tttttttgat attgtgcgct cactgcgtga ggcaatgtat
    30181 gacgaaagta ttgcaggtat acttattcag atagatgcac cagctttaaa ttttgcacag
    30241 gcacaggaac tgagagaaga aatcaagcga tgtaagcaaa aagggaagcc ggtatactgc
    30301 atccttgcat caatgggcaa tatcgaatac tatattgctt cgtcagcatc taaaatatat
    30361 tatgttccca accagccttt tggtattacc ggccttaagg ctgaggtgta ctttttcaaa
    30421 gggctgtttg ataaagttgg tatcaggttt gaagagatta aaaaaggtca gtataaatca
    30481 tttggcgaat cgtttacacg gcagcagatg tcagatgctg ccagggaaaa tattatttca
    30541 ttgctgaaag atttaaatca gcagtacata caggatattg ctcaggacag aaatatctct
    30601 gttcaggaag tggagcaatg tatagcaggt ggtatattaa caccacagct agcaaagtca
    30661 aggggattta ttgatgacat tgcacggccg tatgaagtgt tgcaacgatt aggtgttgaa
    30721 cgatatggcc tgtcatacct ggttacatgc gatagttata ttgaagaaac aatgtttgta
    30781 tatgagtggg gacccaaacc aaagattgct gttatctatg ttgagggaaa tattatacgt
    30841 ggggaagcta aagatgatag ttttctgtca ccagcaatga taggcgatag taactatcga
    30901 aaatatcttg aaaaggtatt tgctgataaa gatgttaagg cagtggtaat ccgcattaat
    30961 tcgggaggag gttcggcaat tgcttctgat ttgatgtggc atacacttgt ggcaatgaaa
    31021 aagcgctata aaaaaccggt agttatatca tttgggaata ttgctgcttc aggaggttat
    31081 tatatagcct gtacaaatga tactatcttt accagcaaag caaccattac cggctcaata
    31141 ggagttatat ttggaaaatt taatttagag gaattgtaca gcaagttagg catcaataaa
    31201 gaaacaatca agatgagcga atttgcagat attttttctg aatcacgagc tttcacagaa
    31261 aaagaaaaac agctaatcca gaagagtgtg gattatacct ataatgagtt tgttcaaaaa
    31321 gttgaaacgg gaaggaatat cccacaagaa agcatcccgt cggtagctga agggaaagta
    31381 tttaccggtg cccaggcttt gcagaacaag cttgctgata aggaaggtgg gcttatagca
    31441 gcaatatcct atgctgcaac gcttgctcat atcaaagggg agtttcaggt tgtgcagttg
    31501 ccgcagaaag atattgactt gcggaagtta ctgaaaggga ctagctttca ggcatcatta
    31561 tcaggcatat tacagtatgt aataaatgcg ttggcattag aaacactttc ccgtgaagaa
    31621 gcattgttgt tgtatcccta tgatattgta attaaatagt ttataaagaa ttttgaatac
    31681 ctggtatgag aaggtttgtg tgactatccc taaaaaacat actatagcca gcatcatagt
    31741 actgtatgtt gttttatttt ctttttccgt gtatgctaaa cccaaagcag ggtttggcat
    31801 tatacgtggg tattcgtctg atgaaaatta cttagcaacg cttattcggg gtcattgtgt
    31861 aaatattgca acgtcacttg gcatatttga tattattaat cctgataatc tgcttcagca
    31921 actgcagcgc ctgccgtgta atgaagagca atgcatactg caatttgcca gtcaggctga
    31981 tttacagatt attgttatgg gtactgtgga gcgctctgca cagggctatg tggtagccat
    32041 taatgcgtat gggcttggta tgccatacta tggcaaacaa ttctgcaatt acaacacaaa
    32101 ggtagcggtt aatatcctgg acagggctga ccgtgaagca agctacattg cagaggaaat
    32161 agcatcacag tttattgcta agctgtgtga gcagtataca gtacctctca cagtgaaaaa
    32221 taatactgtt gctggtgatt atataattag tggtacatat actttttata cactgcagac
    32281 tgtgtacgac aattatacgg tgctgattcc tggcggcaga gttgaggttt ttaacaatac
    32341 agtagctact acagttccag atgattcaat aattcttatt aattttaaaa aggaagctga
    32401 cagtataaaa aacttctact acggcagaaa gaaagaaatt gtctttaaaa agtcacaggt
    32461 tgaagatagc tgtatattga tggcttttac tgttcctgca agtgcgtcaa tgccacttgt
    32521 tgttccgtta tttggttact ataccagtga agactatggg gggcttctcc tgtgggcagg
    32581 caatgtggtg ccctatattg gtcttgaaat ctggggtttt gttaacaggc cttctgagtt
    32641 aaagaaacat cacaatgata tttcgcgtaa ccagatggca tcatattact ttgcctggta
    32701 tatgctcctt gccggtggca tgccttcttt tgttgatgca ttttcacatc aatacctgca
    32761 gcaggcgcgt ttatatcagg gacaggtgcc aaccatgggc tcttcaacta ctgaaatcta
    32821 tcttgctctg ataggggctg gaagtggcat gttttataaa ggcaatcgcc tgtggggcta
    32881 tgtttatttt catctgacca atatatgtgt gtatggattg ctgtataact atgcaaagcc
    32941 acagagctgg gatgagcaga ataaacgcta tactacgggt gcttctcatg caaaagcaat
    33001 gtatgctttt gccgggttac tatcgcttat aaaaattgtg gagatagttc atacagtatc
    33061 agttccatac gcactatcag taaccagtga aatgagctct atagatgttt cccctgattt
    33121 tagaataaac ccttatgaaa aggtaattgg aatatcatgc gcaatgcatt tttaataata
    33181 tgtttgctgc cagtgtatgt aatgctatgc atgaagacag tatatgcaga tgatatgcct
    33241 gttgatgttg ttatcattaa tacgagcata cagacgccaa tgatacaggc tttgagcagt
    33301 ataacgcaaa acgcggtaaa ggaagccttt gaggaatgca agcagcttgt accggctgat
    33361 gctgaaagtt tcatcagcat gacgggtgta caagcaccag gtgatattat tggcaaagca
    33421 cacctgtatg ggatatcgtt actggtatat atgcgggtgt ttctggttgg tccggtgtat
    33481 tactgtgagc tctcatttaa accgctggat gacaaagtag cgtttaagga agaaacagta
    33541 acagtgcagg gaactattgc cagaaatatc cccctgaaag ctaaacggga gattatcaag
    33601 cgccatcagg atgtattaca gtgtactata aaaacaaaaa ttgatgcgcg cacctatgtt
    33661 gtcaatgccg ggcaatggca tggacttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33781 nnnnnnnaag tcaatttttt tccatggaaa caggatcgtg acaaaacgga tgcacagtat
    33841 aggctgcatg tgtatctgtg ggcaaccctt ccagttacgt atacggtggc gttttttgat
    33901 cagctggcgt atgcatacga aagacaccgg tatctaccgc cattttttgc agataaggat
    33961 gtaatggcag ttcttgtttc ggcgatagtt ccgggaggag gtcttttcta taaaggtaat
    34021 agattgtggg gatatggcta ctggctttct gaatttacac tgggaggagt ggttgcatat
    34081 aagcaggctg gtaaccgaaa tatactgctt ggtatattag cagggataaa agtggtggag
    34141 cttttccatg catggcttgc cactccagca tatatggttt attcctatga actatcgcac
    34201 aatactaccc ccattaatgt tgggatgagt agctttggca atgaactgtg tttttacaca
    34261 tatttttcta aaaattattg aagcaaaacc ctgttgcaat ataatggata tacaaactga
    34321 taatagaaag gtaggttatg cctgaaaaag catttcttat attagaggac ggaacaacat
    34381 ttgaggggac tcaatttggc tgggagggtg aagcgttggg tgaggtggtg tttaacacct
    34441 ctatgagtgg gtatcaggag gttttgaccg acccatcata caacggtcag atagttgcca
    34501 tgacgtaccc catgattggt aattatggtg taaatgatga ggatgttgaa tcatcgcgtg
    34561 ttcaggttgc aggatttgta gttaaagaat atagcaaaac atactccaat ttcagagcta
    34621 aacgttcact tgatgactat ctgaaagaat ataaagttcc cggcattgaa ggtgttgata
    34681 cccgaaaatt aacccgccat atacgtgaca aaggggcaat gcgtgcaggc atttttctgg
    34741 ataaggctgg agcacttgaa aaggtgcttt atcatccgcc catggctgga cttgatttaa
    34801 cccgcaatgt catgtgccag aaaccatata catttggtac acaccgtgac acggcgccaa
    34861 ctattgctgt atttgacttt ggtgttaaaa ccaacatttt acggctgctt accgctgaag
    34921 ggtttaatgt tactgtatat ccaggcatca caccgttatc ggaagtatta aaaaatacta
    34981 ctattaaagg tgtgtttttg tcaaatggtc ccggggatcc cgatgcagtt gattatgcaa
    35041 aggtattggt gaaagatatt ctgaagactg agataccctg ttttgggata tgtttagggc
    35101 atcagttgct ggcgcttggt ttgggggcta agacgtataa attgaagttt ggccacaggg
    35161 gtgggaatca gccggtaaaa aatgtattaa cacaaaaagt tgaaataacc tcgcagaatc
    35221 atgggtttgc agtggattat gaatccacaa aaaagattcc ggatattgag ataacacata
    35281 tcaactgtaa cgataccact gttgaaggat tacgtcataa aaaactgcct ttattttgtg
    35341 tacaatatca tcctgaagca aatccaggcc ctcacgattc gcgatatctc tttaaggaat
    35401 tttattcaat ggtggcacag tgttaattaa accctaattg ccaatataac ttcttcagct
    35461 aacgatagcg tatggttgag tataactttt taataaatgg ttgttgacaa atattaatac
    35521 gtacgtagta tttttgattc ccgcatgatc atagtatttc atagtagtat tgattatttt
    35581 tgtataattg tatacattat ttgcaggtat ataactgtgt attattattt tatggaggaa
    35641 tttgtgtgag tgcaaaagta cagaactgga atgaaataac aatacattct gataaagagc
    35701 ctcagcttac tgaaaatgct cgtatagttc ttgaaaagcg gtatctggca aaagatgagg
    35761 aaggcaatgt tattgaaaca ccccgccaga tgtttgagcg cattgcaaaa tacattgcgt
    35821 cagccgattt gttgtatgga aaagatgcaa gacatgttaa tcaggtagca accacatttt
    35881 atgaaatgat ggttgacctg ctttttatac caaacagccc aacattaatg aatgcaggca
    35941 ggccactggg gcagctttcg gcgtgttttg ttttaccagt agaagattcc atggatggta
    36001 tatttgatgc aataaagtat atggcattaa ttcataaaag tggtgggggc actggttttt
    36061 ctttttccag gttgcgaccc aaaaatgatg tagttcagac taccaaaggg gtatccagtg
    36121 gccctgtatc ctttatgaat atattcaatg ctgcaacgga aaccatcaag cagggtggta
    36181 cccgacgtgg tgcaaatatg gcaatattgc atgttacaca tcctgatatc atagaattta
    36241 tccgtgccaa ggaaaaaaat gatgcactta ccaattttaa ctgctcggtg gcagtaagtg
    36301 atgccttcat gaaagcagtt gaaaatgatg aagaatatga tcttattaat ccgcgaagcg
    36361 gaaagtcagc aggaaagcta aaggcacgtg aagtgtttga acttattgtg caaatggcat
    36421 ggaaaaatgg tgagccgggt atagtgttta ttgacagaat aaatgaaaag aatgtattaa
    36481 agaaaattgg tcttattgaa agcaccaatc cctgtggtga gcaaccacta ttaccgtatg
    36541 aatcatgcaa tttaggttca attaacctgg cccgatgtgt ggtgcataat ggtggtgctg
    36601 cagctattga ttatgaatta ttgaaagata taacctataa ggcagtgcat tttcttgata
    36661 atgtaattga tgtaaataag taccctcttc ccatgattga agaaaataca aaagccaacc
    36721 gtaaaattgg acttggcgtt atgggttttg ccgacatgct catagcgctt ggtgtaccat
    36781 ataattctga ggcggcactg agcattgcaa agaatgttat ggcaaccatt caggaagctt
    36841 ctaaagaggc ttcacgtgct ctggcaaagg agcgtgctgc attcccaaac tttccgcttt
    36901 cagagtatgc tgaaaaaggg caggaacctc ttcgcaatgc tactaccacc acaatagctc
    36961 caacgggcac cataagtatt atagccggta catcaagtgg tgttgaacca atatttgccc
    37021 ttgcatatgt ccgcaatgtg cttgataaca ataagcttgt tgagtcaaat ccgctgtttg
    37081 tggaatattg taaacaaaaa gggatatata gcgaagaact gatgcaaaag gttgcaaaaa
    37141 caggttcagt agctcacatc aacgaagtcc ccgatgatat aaaagcaatc tttgttaccg
    37201 cacatgatat tactcctcag tggcatgtac gcattcaggc tgcttttcaa gagtttgttg
    37261 ataatgctgt ttctaaaact gttaatttcc ccaattctgc aacggtacag gatattgaaa
    37321 ccgtatataa actggcgttt gaactgggat gtaagggcat aacagtatac cgtgatggat
    37381 cacgagaatc acaggtattg caggttgaaa aagaaaagcc tctggaacgc ccggtattga
    37441 aaatacagcc gcgcccacgt aaggaagtta cctggggcaa aacattaaag atgaatactg
    37501 gctgtggatc attgtatgtc actatcaacg aagatgagca tggcttgttt gaggtatttg
    37561 ccaccatggg caaagccggt ggttgtgcag caagtcagac ggaggcagtg agcaggttaa
    37621 tctcgctgtc gctgcgttcc gggattgaac cacagcagat tatcaagcag cttaaaggtg
    37681 tgcgctgtcc aaaccaggca tgggttaagg gcggcaagat ttattcctgc gctgatgcaa
    37741 tagcaaaagc catggagcgc tatataaatc ctgatgctga tcaaagtgaa accattgacg
    37801 acatgtataa aaatatagca gaaacaaacg gcaaaggaag tgatacggtc atggtgggtg
    37861 tgtgccctga atgccacggc ccactggaat ttgaatcggg ctgttcagta tgtcgtatgt
    37921 gtggtttttc acgttgcggg taatggtaca tgtttcaggt gtattttact gctgaccttg
    37981 atgaagaatt gagcaacaag cttgaagagc atgcacagag tatatccata ttatatgata
    38041 aaaaagcagt aattgccctg ttcaattctg aggaagaaat ccctgcatgt gctcgcgatt
    38101 atattctttc catacaaaca cttgatgaag aaatctggaa ataccgctat ttagattctt
    38161 tcaatggtat agcagtaacc ccttctgtat ttatttatcc tgcaacaaag ggaaagatgt
    38221 gcgatagttc atacaaatac accattgcca ttaatccaaa ggatgccttt ggtgatggaa
    38281 cccatcccac cacacgcatg tgtattcaaa cacttgagtc cattacaggt actatcgccc
    38341 cagaaaaacg ttcgtcgtat tcactgtgtg atattggcac cggtactgga attattgcca
    38401 tagcagcaga gctttttggg tttggcacta ttgatgcatt tgatattgat ccactggcaa
    38461 tacagcgtgc acaggaaaat gcagcataca atgggtgtaa cagaattaat ttttttgttt
    38521 ctgatattgc aaaagtgaga agtgaaacac agtatacaat tgttgttgca aatttgctat
    38581 cggatattat tgaaaaaaat gctgctgcaa tagcgtctct ggttgaaaaa ggtggaacgt
    38641 taatagccag cggcatccat gatcgctggg cacaaggaat tattgcatta atgcaaaagt
    38701 attgcacact tgttgataaa aacatcatgg acgaatggca ttgcctggtt tttaaaaagt
    38761 aaacgtgatg gtgcatggta atggatagtt gtgaagaaat atatcctggc atttttaaaa
    38821 tagttcagtc caagccacgc agaattgctc ctcctgtcaa catatatatc attgccggcg
    38881 atgatggttt gatctttgat gctggttatg gggatgatac atcaatacac ctggcaggtg
    38941 atgcaattaa ctctatacag aaccgtaata aaaactggcg gattttacgg gtattgccaa
    39001 gccactcgca tcctgatcat ttttcaggat taaagcaatt gaagagtcat tacgggctta
    39061 aggttatgct tactgagcaa atggccagtg ttttatcaaa taaacatgca tatagaaatt
    39121 catataaatt gagcacatta caaaaatacc ggtactctac agtatttgag cgtatatcag
    39181 aattaccact tcaggttgtt ttcagaacca tgtataaaaa agtatatggt attgcattcc
    39241 tgcaatcgca tgacactacg gtaaggccag gcgaaactat tgcaataaat gggtataact
    39301 ttactatcat acatggaccc ggtcattgca atgaccatat catgttatat gataacaaag
    39361 aaggtatcct atttgcaggt gacaatatcc tgcgaagcat ttatacctgg cttggtccgc
    39421 ctcgttccaa cctgcaggaa tattttgata ctcttgaagc gtgcttacgc ctgcccaatt
    39481 taaaactaat attgagcgct cacggcagca ctatccataa tccaaaagaa agaataaaag
    39541 aaattataga ataccgaaag ctacgcacca gacaggtgta ctcccttgtt gcaaatgaaa
    39601 cacatggcat aacatttgcc agacttttaa atagtatata tccccgctca ttagagcgga
    39661 gatggttagc aaaagggtgg attattgtca ctcttgagta tctggtggat aaccatatac
    39721 tgacatttga gggcagaaaa ataaagctgg gggccaatga tattaacaca gtataaaaaa
    39781 attctcttta agtaataaga actatcgccc cctatcccaa tactattatt gtttagccgt
    39841 gaaaagcttt ttccccgtta agaactcaaa agcatctttt gcagtttgtt cgggtatctc
    39901 ttccattggt atatgcccaa ccccctcata cactttaaaa actgcatgag gaaggtcttt
    39961 ttgccattta gggaattcgg tagtataggg tatccagata tcctttgttc cccacatcac
    40021 catgatagga cattgaatat cagtaaggcc ttttgaaaga tcttttcttt tggaatagtc
    40081 tttcattacc ataaaaacat ctatccaggc acttctattt ccttcttttt gcgatagttc
    40141 aaaatatctg tcaactattg catcagtgac tttgctctga tctccatata cttcatggac
    40201 agctttattg ataaaatatc gtggcatgcc tattcgtgca aatggtctga caaaaatatt
    40261 tgtggaaaat ttcaatagcc agggcatttc ctgcggatat ccgacagaat caattaagat
    40321 gagtttttca actttatcag gatgtgtaat ggcaaacttc catgcaataa agcctcctaa
    40381 tgaatttcct gcaatggaaa gtttcttaat acccaaatgt tcaattgctt tgtttagtaa
    40441 atcgacactg tattcaggag tataattgtt agatggtatt tgccctgata atccaaaacc
    40501 ggggatatca aatcggataa ttctgaaatg atccttcaat atttctaccc atccatccca
    40561 tgtatgtaat gatgcacaga ccccatgaat gagcaataaa acaggaccat cttttttgcc
    40621 ttcatctctt atgtgaatga ttgtatcatt gataaggata aagcgggaag catcatttgc
    40681 atattttggt tttagttcat caagtgggat atttcgcatg ccaagggatg agcaacctgt
    40741 agttaaagca atcaatgtaa aaaataacat tccacgtata acagaaaaca tataatacct
    40801 ccggaaaata caattattta tttattgaat cagttatgta aaatttgttt aaaagtgtat
    40861 agcacgtaca ggtttaatga atactgacag gtcggtttat ttgtcaagaa agattcatta
    40921 taagcagcac tataaacaaa aatttttgat ttattaaaag ttggctgtta aatctttcgt
    40981 ctatgcggta atgtaatgaa aaatttcatc aatcagtaaa ctattttttg agtatagtat
    41041 tctttatttg ggacatgctg tgaaattggt gatagaggtt tgcccatttt atggtgtagt
    41101 acaataacta ttatttagtt atctactgaa ttctgaatac tatgcatcag gatgtattga
    41161 ttatagacaa gtttaaaaat gataaaaatt tttatgtctc taataaaaaa attttcttaa
    41221 tgcttgactt tttactcaaa gaatatttta ttatataacg aagagtaagt tttaggttat
    41281 ttacaactct aaattcttac tgttctagcc gcaagtactc ttgcggaaag tcagtttggt
    41341 acaagatgta ccacaagtaa gggttaaggt tatccagaaa agaaagaggg tgaaattccc
    41401 gaaatttcat cctctttctt ttttatacgt aaattaaaaa aacactgtgt gatttcatgg
    41461 aagaatgcat tattactgtg aaagttatcc ccaaatcatc aaaaaacagt gcgcagcttg
    41521 aaaacggtgt tttacgcatt cacctgaatg ctccaccagt tgatggcaaa gctaataaag
    41581 cgctggttga acttttggcg gaaatagtac atgttcccaa atcatctatt gaaattgtcc
    41641 gtggtgaaaa gggaaggaat aagcagataa aaattaaagg gttaaatgaa agtattttta
    41701 taaagcgact tgaacaatat actaataaat gacaaatgat tgtcaggaat aatgttatga
    41761 aaaaaggtat agtatacttt tattgtgtat ttgctcttat cttgttacaa tcatgctcat
    41821 ctgacagtga aaatataaga aaggaaggtt gcattaataa aaatactgta cgtattgtgg
    41881 tattattaac caaaaactct ttgcccacag ggaaattgaa taacgatatt gtatcgcaag
    41941 aagcagtgaa aattgcagat cagcgttgcc agacaattat cagcaatgca atggcaatta
    42001 ataaaaagca gtatgttaaa tctgacagcc atgtagtgtc acggaaactt gtgcttgttg
    42061 aaaaaaggaa agatggctat atggttatag tggattatac gctggatgat gagatagcat
    42121 ctttgttaca atgcaggtaa tggttagttt atgcagttta aagctctgac cacagaagac
    42181 aataccataa ccgtagttga tgacacctat aatgaggcaa tgcattcgct ttccggtgca
    42241 tatcaggaag cactgtatat gcatgttatt ccttcaaagg tgcttactaa acagggtcgg
    42301 gtatatgttc ttgatatagg ttttggcctt ggctataatg tgcttgctct ttgtgctgaa
    42361 gcactgaaat acaaccatac tgttttttat gccatcattt cacttgaaaa gaatactgat
    42421 atactgtctg caatggaata tgtttacttc catgatgaaa gagatacaat ctatgaacta
    42481 ttaaaacaat cagcacgtgg tgaaacagta atgacagatt atttcagcca tagtgtgctg
    42541 tatggagatg cccgccagtt actatcgcat ttacctgtga attactttga tgctgtattt
    42601 catgatccat tctctccagc aaaaaatcct gagttgtgga cagtggattt cttcaagaag
    42661 ctttattcac tctgcaacga tgagtgtatt gttacaacct attcggctgc aatgcatata
    42721 cgaactgcaa tgaaacaggc aggttttatt gttggcaaag gaccagaggt gggcagaaaa
    42781 aaagaaggaa ctatcgccac taaaaaaaat atgcacattg tgcctcttga tgacagctac
    42841 tggttatatt tggaaacaaa tcctaaagca gtaccattta ctgatccaac gttatgcgaa
    42901 acaccagaag aaatacgaaa agggcgaaag cttcacatgg ctgcgtttaa aaaaggtctt
    42961 caagctcctt aataactgag gtttggtttt gttttttttc ttcctgtgat actgtgccag
    43021 taacatttat ttgctggcca ataccaatag gcacctcgcc atctttaaat tcaatatcac
    43081 ctgccaggtc accttcagaa agccggtaag aaagtgcaca ggcataaaag taagccaggt
    43141 gtattgcttc atacaccttt ttgttttgta acagatgaac tgcttcaacg ttgacatcaa
    43201 actcaagaaa atcttgaggg ttttcaataa ttttattttc ttcatccatg tcacggataa
    43261 gaacatcaag ttcagcggca atcctgtcca tgatggcttt tgtggtcatg cgcatatttc
    43321 ttgattcaac ttttttcttt ttttcaagct gtgttaagcg ttttgtatat tcaatatact
    43381 gtgcattgct caaaggcttt tcagcacgta tgcgctcgta ggtttcaaga acatgtttgt
    43441 attcttcaaa gctttcagtt actttgcgca attcattata gcaatctgta agcttgtttt
    43501 tgtaatctat tttacctgca tcggtaaact gtatattata ttttatttta tttgtggtga
    43561 gtataatgga aaattcatcg tcaaattttt taaaaagtgc ataggtaata atagctttgt
    43621 ctgctggatt tgcctgtaca tacgaatccc tggaaaatag tgctgctata tcattggtgc
    43681 ttatattcat aagcgaaagc ccttttgcga tgtgttgtgg aatttctgaa agttctttct
    43741 gctcttcctg tgtttgttgt tgttcttctt ctttttcacc aggctttact gctttacgtt
    43801 tgccaggcag atcgctatca tttatttgta agatagttct gatatagggg cttgttatat
    43861 caatatagac attatagtaa tgacagaaca ggatatgaag ccggggtaaa aatttgtaaa
    43921 atgtaatata caggctgttg cggatatatt tcctcttttc atacgatgta gatttttgat
    43981 tttctactga tgcctgtatt tctaatgcat tgcggtaagc taattcaatt aattcgatgt
    44041 acggataaag aatgtagagg cttttataca tgggtaggag atagtttctt aatttttgca
    44101 catgtaccat atcatttgat tgttgaaggg cttcaatttc attaaatcgt gttctgtcaa
    44161 aaatatttga aatcatcaca atcaattcgt aataaagggg gtttatctga tccagtttgg
    44221 ctataacctg cttgctgtac tgtggattga agatgtgata atactgtact tgaaattgaa
    44281 gaagtgctgt tttatatgta tggataaggt tatccataaa acgaggttta cagtactcag
    44341 cagaaagtga agtaactcca agaaagaaca ggcgaaggcg aataagtaac ctggaaagtt
    44401 ttttttgcag ccaggtttca aaaatttcat ctgattgtat ttttggctgc tttgattctt
    44461 tgggtggtgt ttttacctgt gattgtcgta ctactcttga agcaggtttt atagttttac
    44521 tgttcctggt ggttggcttt tcacgctcct ggataacttt ccctcccgcc tgaacaaatt
    44581 tttcaaacat ttgcttgcgg gttctctcat caagttcatc gactttgatg ttttcttttg
    44641 ttttgtctat ctttttcatt gagatactct ttaattaaat atacggatac tatgtgatgt
    44701 atgtcaacaa tattatatgg ggaaaataac ttgaaaaatt tagtatgtat atttcaatag
    44761 tgaacagggg tggtaaagtg ttatggttac atttcagctc cagacaacac ggaaagaaga
    44821 atttattgat atcactgcaa aggttgcaga agcagtggca aaagaaaatg taaaaagtgg
    44881 gttatgctgt gtgtacattc cccataccac ggcggcggta actataaatg aaaatgcaga
    44941 ccctactgtt cctaaagaca ttcttatggg gttgtcgcac cttaaattag aatcattgca
    45001 ttttgcccat gtggagggca attcacctgc tcatataaag tcttctgtta tagggtgttc
    45061 tactattata attgttgaaa ataactgttt acaactgggt acctggcagg ggatattctt
    45121 ctgtgaattt gacggcccgc gtacacgaaa ggtcatggtt cagtgtattg gaacataaca
    45181 gtgtaatttt ttgtttttaa ctgattttgc actacactgt aactatagaa agtaaatggc
    45241 attctgcttt ttatagccat atgctatgca tgctgcagct ttctaataaa ctatcgtgaa
    45301 aaaaagtata ttgaggtgca tgatgaatta ttttaccgga accgaagatt atgttatttc
    45361 ccaggaactt aaagataatg taaatgtgtc gatagctctg ggacggccgt tgctggtaaa
    45421 aggtgaaccg ggcacaggca aaacgttgct tgcacattct attgcaaaag gactaggtaa
    45481 aaggctttta gtatggaata ttaaatcaac aacaaaggct caggaagggc tttatgtgta
    45541 tgatacagtg cagcgtttga atgatgctcg atttggcgat aaggatgttt ctaatattaa
    45601 acagtatatt aaattaggga agttaggcga agcgtttgca tcagaagacc aggtggtact
    45661 gttaattgat gaaattgaca aggctgatat tgagtttccc aatgacctgt tacatgagct
    45721 tgacattatg aatttttata tacctgaaac aggcgaaaca ataacagcaa aacaccgccc
    45781 tattgtaatt attacatcca acagcgagaa agagttgcct gatccatttt tacgaagatg
    45841 tgtatttcat tatatagcat ttcctgatga ggaacttatg gaaaagattg tgcgggtgca
    45901 tttccccaat attgaaaaaa aacttttacg tgaatgtttg aagaaatttt actggatacg
    45961 gcagtttgat atgttgcgga aaaaaccctc aaccagcgag cttgttgact ggattcaggc
    46021 gcttatggtt ggtggagtac ctgtcaaaaa gatagaagat gaattgcctt tcattggtac
    46081 tcttttgaag aagaaagaag acattgatgt agttgttaat tcagggaaaa cacattatgc
    46141 atataatagc agtataagga cacgcgatgt ttattaattt tttcttcaat cttaaagcac
    46201 agggtgtgcc cgtatcaata catgagtgga ttgcactgca tgaagcgttg gctttaaatc
    46261 tgaatgatac aagcttaact aaattttatt atatggcacg ggcaatacta gtaaaaaatg
    46321 aggtgtttta cgatagatac gatattgcat tccttgatac attcaaggat gtagaaacta
    46381 cggatgaact gcttgaaaag attttggaag gcttaaaaaa agtaaaagag ttacacctca
    46441 ccgaagagga aaagcgtcag attgaaaagc tgaatttaga tgaaatattg cggaactttg
    46501 aagagcaact gcgccagggg cactacaaga atcatgttgg tggtaataaa gctattggta
    46561 ctggtggacg ttccacgcag ggtgcctggg ggtataatcc ggcaggcatc aggattggac
    46621 agggtgtttc acgtcacaaa agtgcagtac agatagctga aaagcgtgag tttaagaatt
    46681 actctaacca ggtggttctt gacactcgac agatgcgggt ggcattatca catctgcgtt
    46741 cgcttttgcc tattggccct gaagaaaagt tagatgttga acagaccgtt gatgcaacca
    46801 gccgcaatgc cggtgagtta gagttaatat ggaagaaaaa ggaaaaaaaa gcttcaaagc
    46861 ttattttact catggacgtt ggtggttcaa tgacgccgta tgcacggtta gttgagcgcc
    46921 ttttttcagc tgcatcatca cagataagca agttaaaata ttactatttc cacaactgtg
    46981 tgtatcagga cctgtggaaa gatattgaac gttctgaatc aataagcact gccgattttt
    47041 taaagaatgc tgataaggat tataaactta tcattgttgg tgatgctgaa atggctcctt
    47101 cagagcttac ctgggtaaac ggagctatcg attactggta tcacaatgat ataccaggga
    47161 ttgtgtggct acaacgcttg cgtgaaacgt ttaagcattc aatatggctt aatccaatcc
    47221 ccaaacgctc atggtattat atacagacaa cacgcatgat taagaatatc ttccctatgt
    47281 ttgaacttac cttagaaggg cttgatgaag gaatcaaaca cctggtatca ggaaaggctt
    47341 tgcagaaagt ggtgtgaaat ataatgctat atagtgccca aaatttggaa tcgggtgtat
    47401 atagtcctgg tattaccgta atgttttttg cagttctaat tttgctacag ctctttcacg
    47461 gtattcccgc gctttctgat ggtcagggtc tattaaaagc actttatccc attcagcaat
    47521 agcagcttca aaattatcct gttcatagta tgctacacca ttattatagt aggtgttaat
    47581 attaccttcc agttttgatt tgatggttga taaataatta gaagtattgc gataacctgg
    47641 ctcttcattg tccactcttt taaataatcg ataggctgat atgaggtccc cattacggta
    47701 tgccttttca ccattggcat acattttgtt aagattctcc attctataaa tatctttaag
    47761 aatattcctt gctttttcat cccatggatt tacttttagt gctttgttgc agtatgtttt
    47821 tgctgtatca aacttatttt ttttacgagc ttcttcagct tttgaagtgt agggataaga
    47881 agcgttatct aactcatttt taatgcgttt ttttaacact actaattcga tattgtcagg
    47941 attaaaaatt tctgcattgt ttgcactttt gtaagctgta atatattgct ttttgtcaaa
    48001 ttgtttctta gcttgtgaat agtaatagtc atattttttt ttggcatatg atgagttgcg
    48061 aatatttgca atttgtgttt taacatcttt atactccgga tcacgttttt cagcttccag
    48121 aaattgcagc agtgcgtttt caaggtcacc tttacgctta taattaatac cagcatcata
    48181 atatttttga gcgcgtttct gcaaggcttc tgtagtaatt attaaatact ggcgtgctcc
    48241 agtatgttcg gggtcaatgg aaagtactgt ttcaaaacgt tcctttgcct gtgcataatt
    48301 ccctttttta tacagttgta taccttcata ataattctca ttcatttttt tcttttgttg
    48361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    48421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttttatacag ttgtatacct
    48481 tcataataat tctcattcat ttttttcttt tgttggcgat atatagtagc acaccccaag
    48541 ctgaaaagta tggtaagaag taaaaaaaac gaaaaaattc tttttgattt taaattcccc
    48601 atatctctat tactcctttg taagggtttc atataatatt tatgaattag catatctggt
    48661 tgtcaagctt gattagttag agtttacact ataatttata ttaatcatag tacgtaatta
    48721 ttcaattatt tttatattga caatgaatct tttgaatgtt ttatcaaatc acataacatt
    48781 aagtatgggt ttagaaattt ctagttagat ggagataatt atttaataat gggaaacggc
    48841 aattgtgttg ttcaggtacg tgatattgtt aagacattta ccatgaaaaa aggtgctttg
    48901 gggaagaagt ccattgttaa ggcagttgat agagttagtt tatcgattgt tgagggaacc
    48961 acctatgggc ttgttggtga aagcggatgt ggcaaaacaa cgctggcacg tacattacta
    49021 cgcctcattg agcctgacag tggtgaaata gatattttaa atacaccatt gcacagattg
    49081 tcaaaagatg gtttgcgcag gttcaggaaa aatatgcaaa tagtattcca ggatccgtat
    49141 ggttcgctta atccgcgtat gactgttgaa aagattgtat ctgaatctct gcgtattcac
    49201 caaagctttt caaaagagga agtgaaggaa aaaacatata atctgttaca aatggttggc
    49261 cttgatgcag gccatgcttc acgataccct catgagttca gtggagggca acggcagcgt
    49321 atttgcattg ctcgggctct ggcattgagc ccttcatttg tggttcttga tgaacctata
    49381 tcagctcttg atgtatcaat tcaaggacag atattaaatt tgctgaatga tttgcaggaa
    49441 acgttaggtg tggcatatct ctttgttgcg cataatctgg cagttgtaaa gcatataagc
    49501 accagagttg gggtaatgta tctgggcaaa attgttgaag aaaatgtttc cgataaactg
    49561 tataaaaaac cactgcaccc atatactcgt agtttaattg cttcgattcc ggatataaaa
    49621 ccttccaagc attcattctc agttgttaag ggtgaaatcc cttcgcctga aaatccccca
    49681 aaaggatgtc atttccatcc acgatgtcag tacgcaatgc ctgtatgcag tgaagcatat
    49741 cctgcaacag ttacagtaca tggagcaaag gtagcatgtt ttctttacca gtgaagactg
    49801 caatgttcgt tttgctatcc tgcagttttt gttttgcttc agatgaatta acaggaacta
    49861 tcgcacaaaa tccaaaatat actatttatg aaaaggatgg agtacttttt tgcacaggca
    49921 acactgaagc cgccctgcat aataataaag gtgctgaaaa gcttgtagcc aatgatctac
    49981 aaggagcaat agatatattt aaagaaggct tgaagcatgc tcccctgttt tatccttttt
    50041 tatataattg tggtgttgcc tgtatgcgta aaagggaata cgatgctgct cgtatatacc
    50101 tggaaaaggc aatgcacatt gtgcccgaaa atccaaagat atatatatta atgggtgagc
    50161 tgtatgcatt aacaggtaga gaaggtgatg ctcttatctt attcagaaaa gctttgcaga
    50221 ttaaccgcaa agagcttcag gcaataataa aaataggaaa tatttatgtt gatcgaaatc
    50281 aactggaatt ggccgtaaca ttttttgatg ctgctttaaa aattaatcca cagtatcctg
    50341 atgctgtcat agggtatgca aaaattcatt tcaaaaaagg tgaatattat aaagcactta
    50401 tatggctgaa gtcaatagat gtggctaaag gggattatga caaggcatat cattactatt
    50461 ttgccgaaag ctcctttaag ctgaaagatt ataaaactgc atatgagcag tatgaaaaat
    50521 tattgcagtt taaaaatgat gttttctttc ttacccactc atatgcactg atagagcata
    50581 agatgaatct ttcacgtcag tttgccgaat tggaacatgt agaatagtat attgtctgct
    50641 aatgttttta gtacacagtt attatattgt ttgagtagat atcacttcat agtaaaatta
    50701 gatatttcca gaaatatttt tctttgtaaa ttttcaagca tcctggaaac ctgagtattg
    50761 agttctctat cggtgcttct tttttgtata tatttattaa tttcatttat ttcactacgc
    50821 aggattccgt tcactctttc aagagtagca agataatcag cagagccctg tgattgtatg
    50881 tgtaattttc ttttttttat ttcttcaagc aactcctgtt ctttttcttt ttttgttttt
    50941 tcgttcagga taatctggtc aagagcctta tgtgcttcaa caagtgaatt ctttttattg
    51001 gatagataaa acagtggaat gtgtgcatga ttggcaagta tgtgcggcag gggattacag
    51061 gtaaattctt catgtattgt agaataaagt ttttcatcta tgatggcatg gttatataaa
    51121 atatcatggg cgtattgtgc atcagatact attataatat cctgccctga ttgtattacc
    51181 ctggcattat aggactgaat aattgcttta aatggtatat gtgaagtatc attatactgc
    51241 tgtatatttt gtacgatggc tatcagggca tcagcaatat tcaaaggatt attagaatat
    51301 actgtaatta aaccaggcaa cagtgcatgg attacaaggt tatttttttt acagtgattc
    51361 aggataaggg attgtatagt attgagtgtg gtcgcaagtt tttcaagtaa aattggattt
    51421 gtatctccat atgtcgttat tgttattata aagaaatcgc ttaaagtatc aattttgcct
    51481 ggtttgattg ttaaagtagc ttcggcctga gaaatatcaa ttccattctt tctgtacatg
    51541 ttaccaataa aattgacaat gtcctcagaa atttcagcgg gtagattctg taatattgat
    51601 actgtttcag gagcaattgc atattcttcc tttaataagg ataatagaaa atatgaaaat
    51661 tctggtgtat tgtaattatg aaatatatat agcatttctg tctggatgtc cctgtttttt
    51721 atgattagca tttctttaat atattttgca acctccttat tgttgagttg taataatatt
    51781 gcgattgcat atatacggat gcgaatccct gattctttta aacaatctaa gaggaactga
    51841 gacaatgctc ttttattgaa atctcttaag cggggtatga tgctgctaat tgattgtaat
    51901 attgtttctt ttatagaaat ttcatttgtt tcagtaaaaa gtgttataag ccattccatc
    51961 tctttttcat cgccacagtg gccaatatta cgtatggcgg cttgcttaat ggctatatca
    52021 ttatatgtca gtgcaatgtt ttttaggatt tcagatgaat ttttatattg tgcagtttca
    52081 tcctgtaaca ggagatgtaa ggcagtaact atcacctcag ggtccagatt tgctgtattc
    52141 ttcaaaagtt caaaaaggat agaggctgcc tgctggtctg tataatgtgc caggtatttg
    52201 agtgagaaaa gctggtcgtt acggtcgcct gataataaca ttaattccag ttcaccaagc
    52261 atataagggt atttcatggt ggataatgtt tgtatgcatg catcaaatag tttctcatca
    52321 cgatactttt ttagataatt atagattttt acaaggaggg ttgcagctga ttgaatattt
    52381 aatttggcta ttatattaca taacctgata attcgttcct gtaaaaatcg cttttcgaag
    52441 tcaattgttt ctaaccgtga ggcagtaatt tccctgcttt tgggatccct gtcatagagc
    52501 atttcaacca atgatgaaaa tggatctcga atagagctgt caatgaaagg caaataagtg
    52561 gttaaaagag tgatgatttt atgtatttca tcatttttct catgcttaag ataatgaata
    52621 attctgttta aaatttgtgg agaaaatttt aacagtataa aattccttac ctccctgaag
    52681 atttcggggt aatttttttc ttctacatct gatatcatat aattttttct atacagttca
    52741 aagaaagaat ccaatgtgac ggtgaaataa ttaaaaagtt tatcttttaa taatgtactg
    52801 cttctgtcgc tctgaaacga ttcaaaatag tttataatgg tatcaacaat tattgtatta
    52861 tgatggggcg atacagttcc atatgaaatg agtgccctga ttaacggttc tggatcccgt
    52921 aataacatga tggtatgctg gagtaaaaga agtagttctt ctttttcatt ttcattttgg
    52981 ccaccaagta tttttagtac ataatcattg gtctccctgt gtttagatgt aataagagca
    53041 ttcaaacatg agattttcac ccgtgttgaa aattcatcaa aatgaggtag catcttggat
    53101 aaaaataccc tgagttctat aataagattt tcttcttttg atgaatctga tgcttcattt
    53161 ttttgagtct tcttggtaat gcaatagtcg cttataaatg caatggcgtc aaaaattccc
    53221 agaagagtat attcattctg attaattaat gaactcagta catgaatatc atcgtaaatt
    53281 gacaatgccc gtattgcata gtaccggatt acctcatgag ttcctgtctc attaattatg
    53341 ctttttaatg tagcaaggag tttactgtca ggatgccgta atattctcag cgtttcaagg
    53401 cactgtatta ctattgtatg gtttgattct ttattgaaat tatccataat ataattattt
    53461 aaaagctcta ccgtctggct attcccatat gttgcaaggt atgtgcagat ctggatcttt
    53521 tcatcatcgg ttattgagtg ctggaataaa gcatggaaaa ggtctgtgaa tcctggcagt
    53581 ttatagtggg ttattgtacg tattgcactg aatcgaatag ctggctctgc agatagttct
    53641 gcgcatagta tattatgtgc aatatcactt tttgattgtg caataaaagg atgaaggcat
    53701 atacgtaagt atggtgtaaa ctttagattt ttagtaattt ccagtttaat tgattgcaaa
    53761 atatcacgtt ccatacagta ccttatatac agggatttat tgatttattg caggtaaaat
    53821 atatactagt atggcatagt atgtaaatca gaatttttcc tgcagagaat gtaaagttaa
    53881 aaaaaagctt gaaattgtaa gcatatgata ctatattgat taattgaggt agttggtttt
    53941 ctgctttatt gttaacgatt gtgtgtaaag cgggtggcaa aagccactct tttttttagt
    54001 caatgttata tatcaataaa catgagcatt attatgaaac tcaatgaaag ccttgagtta
    54061 ataattaaca atgtggctca cgacctgggc tatagtgtat atgacagcgt tcttgtttta
    54121 catgggaaga caccgcgcat tactgttaaa attgatcaca ttaatggcat atcgcatctt
    54181 gattgtgaaa aattcagcaa ggagttgtcc catcgtatag atgaaggaca gttgcttgat
    54241 gactatacta ttgaaatttc ttcaccggga ataaagcgag aactcagaac tattgaagac
    54301 tttctacggt ttaccggagc gcctgttaaa ggtatttatc aggaggatga tgccaggaaa
    54361 acattcaaag gcacgcttgt agggatagag ggggagatag ttactattaa tgaaaccaat
    54421 aaagtattga ctctttcata ttctcaaatc ataaaagcaa atttagatta ttaaaggtga
    54481 atagtatgag tggaaacttt tttgaagcac tgcatcagat tgctactgaa aaagggataa
    54541 cccgtgagat gattgaggag atagttcagt cagcgatgct gtctgcatat aagaaacaat
    54601 acggcattac caaacctgta gatattacct ttgatagaga aagaaatacc gtaaagatta
    54661 tttcgcgaaa aatggtggta cgggtgccta aaaattatgc tgaagaaata tcactggaag
    54721 aagcgcaaaa gattaaacct gatgcaaagt ttggtgatga ggtcatcgtg gaagaaaatc
    54781 ctcttgaaac atttgggcgt atagctgcac aaacagcaaa gcaggttatt ttgcaaaaga
    54841 ttaaagaggc agagaaaaat attgtttatg atgagtataa agacaaagta aatgaattaa
    54901 taaatggcta tgtccagcgt aaaacaaaag aagcactcta tattgattta ggaaaaactg
    54961 aaggtataat tcctgcacgt gagcagtcgc cacttgaaca ttataaaata ggcgacagag
    55021 ttaaagctat agtgttgagt gtacagaaaa acagcaaagg tcccagtgta gtactttccc
    55081 gtgctcatcc aaaatttgtt gaaaaacttt ttgaaatgga aattcccgaa atttatgatg
    55141 gcatagttaa aatagtaaat attgtacggg agccgggaat gcgcaccaag gtagccgtaa
    55201 ccagtgacag ggatgacatt gatagcgtgg gtgcatgtgt tgggatgaaa ggtgtacgga
    55261 ttcaatcaat tgtccgtgag ctagaaggtg aaaagattga tgttatagaa tattcaccca
    55321 ataaaaaggt aatggcttca aatgcactga ctccggcaaa ggtcaaagaa attattgaaa
    55381 ccagggatgg tggagttata gcggttgttg acaatgatca gtaccgtctg gctatcggga
    55441 aaaacggtca caatgcgcgg ctggctgcca aattatgcgg gttcaatatc gacattaaaa
    55501 ctgaggacca gtatcgtgaa ttcttaagtt caagtgaatc acgtgcactg gttgagcagc
    55561 tatttgcaac aacatcagaa ggcgaaacac cgctggaaga attaccaggc cttgatgctc
    55621 gaaccattac tctgcttgaa gcgggtggaa ttttctctgt tgaggatctg gttgaaaaat
    55681 ctgtagagga tctgatgaat attgatggta ttggtgaaaa aacagctcaa aagataatta
    55741 atataataca agaatatgtt gactttgaag aagaagatta tgaagatgag caggaagata
    55801 agcaggaata gtacagatag tgccatttga acggaagtaa gagtctatca tttataaagg
    55861 tagggtttcc gggttgctct cttccattga tatggcataa gaacaggcta ataatttaat
    55921 ctaataggga tttgttaatg aaagctatcg atgtggcaac aaaaaatcat gtttcaacag
    55981 atgatgttat aaaaatctgt aaggatcttg gcatcccctg tacagatgat cagtctgaac
    56041 tggcaaatga cgatgtcttt cttatagaaa agaagattca gataataaaa gaacagagag
    56101 cccaggaagc aaagaaactt attcagcaag cggaattaaa gaagaaaata aaactaaaaa
    56161 gaaaagtaca tgttgcaaaa gagttaaaaa aagaagctga ggcaaaagaa gacgaagcta
    56221 ctgaagaagt tgttgaacat aaagaggatt caaaagaagt tgctccgaaa caccgggcag
    56281 agacgggagt agttgaaaaa gaaaagcagg aaaaaaagga gcgatttgat aaaaaagaaa
    56341 gacatggcaa gcctaaggac aggttcccca aaaaagattt caggaaagat gataaacaaa
    56401 ggccaggggc tgaagaaaag gaacaaagac ctgaaagaca gcatgcgcca acagtagttg
    56461 aaaaaagaga tgttccggtt attgataaag aagttgtgga tttgcagaaa cggcaaaagg
    56521 aaaaaggaaa agccaaagaa aaagacaggg ataaagataa acgtttctta aaaaaagaaa
    56581 aagaagaact ggaagaaaaa ttattgctcc tgaagcgaaa aaaagaatct cagccacggg
    56641 aagctgttgc tccaaaagaa atagagatta ccgaaacaat taccgttggg gatcttgcta
    56701 aaaagatgaa tatcaaagcc agtgaagtaa ttgccaagct tatgtcaatg ggtgttatgg
    56761 ctacaattaa tcaggtcatt gatgctgata ctgcttcaat actggcaagt gaatatggta
    56821 ctgaagtaaa agtggtctca ttatatgaag aaacagttat caaagttgat gaagaggatc
    56881 ggccccagga ttatgtgtcc cggccaccta ttgttactgt tatgggacat gtagaccatg
    56941 gtaaaacaaa attgcttgat gcaatacgcg aaaccaatgt tgtcgcaacg gaacatggag
    57001 ggattacaca gcatataggc gcatacaggg taaaggttga agataaatat gttacatttc
    57061 ttgatactcc ggggcatgaa gcgtttacca caatgcgtgc gcgtggtgca agtgtaaccg
    57121 acattgttgt gctggttgta gcagcagatg atggtgttat gccacagact atagaagcaa
    57181 taaatcatgc taaagctgca aatgtgccta ttatagtagc aataaataaa atagaccttc
    57241 ctgaagctaa cccaaacagg gtgaaacagg agttaaccgc atacgaactt atacctgaag
    57301 agtggggagg cacaacgctt tttgctgaaa ttagtgcaaa acaaaaaata aatatccatg
    57361 agcttttgga attaatatta atccaggcag agatgcttga gcttaaggcc aatcctaaac
    57421 tccgtgcaag gggtgttgtt attgagtcca aacttgaccc gggaagaggt cctattgcaa
    57481 cggtgctgat tcagaatggc acattgcatg tgggtgatcc gtttgtggtt ggtctatatt
    57541 caggaagggt gcgtgcaatg ttcactgata ccggtgaaca ggtacaggag gccactcctt
    57601 caatgccggt tgaagttctt gggatcagcg gagtgccatc ggcaggtgat ccgttccagg
    57661 tagttgaatc agaaaaatat gcacgccaga ttgcccaaaa gcgccaggaa ctggttaggc
    57721 ttgaaagtgc tcagaaagtt aagaaggtaa ctctggaaga cctgaataca atgattaagg
    57781 aaggcgagat caaagaactc aagattgtaa tcaaagcaga tgttgatggt tcagttcagg
    57841 cattgcgcga ttcgttgcag aagctttcca ccaatgatat tcgtgttaaa gtaattcatg
    57901 cagcaacggg cggcattaat gaatcggatg ttatgctggc ttcggcttca aatgctatca
    57961 taataggata tcaggtaaga ccatcagcaa aggttgcaga aattgccgag aaagaaagtg
    58021 tgtcaattaa gtattataat atcatctatg atgccattaa tgatgtcagg gcagccatgg
    58081 aaggtatgct gtcgcctgaa atgaaagagg agattacatc aacaggtgag gttaaacagg
    58141 ttttcaaaat cagcaaggtt ggaactgtgg caggagctat tgtgttaacg gggcgattaa
    58201 agcgaagcga taacatcaga ctgattcgtg atggtgttgt tgtgtatgat ggtaagctta
    58261 attcattgaa acgttttaaa aatgatgcag gtgaggttga agcaggtcag gaatgtggtt
    58321 ttacggtaga aaatttcaat gatatcaaag aaggcgataa ctttgaatca tatcagatag
    58381 tagaaataaa aaagaaatta gaggattcct cacggggata aaccgccaac atgggattca
    58441 gaaaacagaa attagaaaca cagattaaaa aacttgttgg aacgttaata gtaacggaga
    58501 ttaaagatcc acgtatagga tttgtaacag tgacaaatgt tgaattgagt aaagattatg
    58561 catatgcaga tgtgtatgtt tcagtgctgg gtgatgaaaa cactaaaaag aagtctatgg
    58621 caggtctgca atctgcgcgt gggttcatcc agtacagagt tgggaaagct ctgagtatcc
    58681 gcactattcc agaaattcgt ttccatttag atacatctat agaagagggt gtggatatgg
    58741 tgaaccttct ggagaaatta gaaaaagaat cttcaaagaa taatagcgga agcagtgata
    58801 cgtaatatat gagtgttcca catataaagg attgtgtgtt gcttattgac aagccttctg
    58861 gctatacttc ttttgatatt atacatcgta tcagaaaaac actgaaagtg aaaaagtgtg
    58921 gccatgcagg tacgctggat aaatttgcat caggtctttt aattgtatgc ctgggatggg
    58981 caacgcggct ggtgcctttt gttatgggta tggacaagcg gtatattgcc acaatgcaat
    59041 taggcgtggc aactgacaca catgacagtg agggtcaggt tatttctaaa agcaatattc
    59101 agtgcacagt tgaggatata caatcatgcc tgcaaaccta ttttacaggg gaaattatgc
    59161 aggttcctcc tgattattcc gccattaaaa ttaacgggaa gcgttcatca gatagggtgc
    59221 gcaaagggga attggtagcg atagctccaa gaccagtcca tattcataaa attacagtga
    59281 tagattttga tacagcgtgt tcacagctta cacttgacat tacatgtagc aaggggacct
    59341 atatcagagc actggctcga gatctgggtt tgcgattaca cacaggggca catgttatag
    59401 cgttgcgaag aattgccatt ggggatttta gcgttactga tgctctaaca gtggaacaat
    59461 gcatagagct atcgcacaaa aatattgagg atatgcgcgg aattgttaca cctttcaggg
    59521 cacttggtaa ctttggtgtt atagcagtaa aaccagattc attgataaag attaaacatg
    59581 gcaagctact tggctatgat gatattaccc atgttgccgg tgagcctgta ttcagggtag
    59641 tgcctgaggg gactgaagac ttagtggcac tggctgtgaa agatgaaaaa gggtacaggt
    59701 gtaaaaaagt ttttattgaa gagcttgata aaattgatac aatgaaataa aagtgttccc
    59761 cgctttttat aatttatttc ttgactgatt ttgactgcaa atagcactgt gaattataag
    59821 acttacagtg aatataataa attagaatat agtgattgta aggaggacta aaaagtcgta
    59881 tggcagaaaa aagcgagatt atcaacaaat accagttgca tgacaaggat accggatcag
    59941 cagaggttca aattgccctt ttaactgaga ggattaacca tctgaccgaa catttcaaag
    60001 tacatgttaa agaccatcac tcacgacgtg gattgctgaa gcttgtaggc aaacgcagac
    60061 gacttcttga ttatcttaag aagaaggatt taacaaagta cagagaactg attaaatcac
    60121 ttggcatacg aaggtaaagg gtacaaaaga ttttaaaata agtagttaag gattatacaa
    60181 tgaataagga atttgtagca catattggag atgatgtaat cctgtttaat accggaatac
    60241 ttgcaaagca ggcggatggt gccgtggcag tttcgtgtgg tgaaacgata gtatttgctt
    60301 cggctgttgc gtcaaagaag gttgtggaag gacaggattt tttcccgttg acggtcgatt
    60361 atcgggaaaa acattactca gcgggcaaga tacccggtgg gtttattaaa cgtgaaggaa
    60421 gacccagtga caaagaaatt ttaaccagca ggctggctga caggcccata cggcctttat
    60481 tcccggatga ttttgtcaat gaggtgcagg ttattattta tgtgctttct gctgacagag
    60541 agcatcagcc ggatgtgatg gcaataaatg cagcatcagc agcactcatt atatcaggta
    60601 taccttttaa ggggcctgtt ggggctgtga gggttggcag aattaatggg cagtggaaga
    60661 taaatccata ttttcaggaa atggaacaca gtgatattga tcttgttgtg gctggaacaa
    60721 aaaaagctgt gaccatgatc gaagggcagg caaagaattt aactgaagat gaaatgatag
    60781 aagctgttga atttgcccat gccaatatca taaaactgtg tgaagtacag gaagcattaa
    60841 aagaagcctg tggcaagcca ctgatgtcct atgaaccaaa attaagcgat aaggaacttg
    60901 agaaagttat acgagaacga ttttttgctg acatagaaca gttaaaagat gttcatgaaa
    60961 agaaggcacg agagcaggca aaagacacac tgattacaac aataaccagt cagttgcagg
    61021 aacaattccc tgataccatt aagctggtct ctgaaattgt agacaactta gatgccgaag
    61081 tggtgcgctc gcgtatttta aatgagggca agcgcgcaga tggcagggga ctcaaagaca
    61141 ttagaccaat tgatatcatg attggcattt tgccacgcgc acatggttca gcactgttta
    61201 ctcgtggaca gacacagagt ttggggatag taaccctggg aacagttgct gattcccagc
    61261 gtattgatag tattgattta attgaggaat catataagcg gtttatgctt cactactatt
    61321 tccctccttt cagtgttggc gaagttggaa gggttggcgg tgtgggtcgt cgtgagattg
    61381 gccatggtat gttagctgaa cgagcactgg aatatgctat tcctgatgaa gatgtatttc
    61441 cctataccat ccgtgttgtt tcagaaatac ttgaatctaa tggttcttca tcaatggcca
    61501 ctgtgtgttc aggttcgctg gcattgttca acgctggtgt gcccatgaaa gctccggttg
    61561 ccggtattgc aatgggtctg gtacttgagg gcgaccgcta tgccatttta agtgatatcc
    61621 agggtattga agaccatctg ggcgatatgg attttaaagt tgccggtact gcaaccggta
    61681 ttactgcatt tcagatggat ataaagattg agggtattac cacagagata atgcgcaaag
    61741 cattggagca ggcaaaagaa ggcaggctct atatactgga aaagatgaat gaagtcatat
    61801 cagaacctgc aaaggaacta tcgcctcatg caccaaaaat tgccgttatg aagatcgaaa
    61861 ttgaaaagat tggtgaggtg attgggcccg gcggcagagt catcaagaag atcatagaag
    61921 aatcaggtgc cgatatcaat attgagcctg atggtaccat attcatttct gccagtgaac
    61981 aggatgcaat tgacaaggca cagctttcta ttaaaggatt ggttgaagag gtggaagttg
    62041 gtgctatcta caaaggtatt gtaaagcgaa ttactgattt tggagcgttt gtagaaattt
    62101 tgcctggcaa acaggggctg gtacatattt caaagcttga tcactcgcgg gttgaaaagg
    62161 tgaaggatat cttgaacgaa ggtgatgagg tgctggtcaa ggtgattgga attgatgagc
    62221 aggggcgcat caacttgagc cgcaaagacg ctatgaagaa gcattagtcc aaaaaatggt
    62281 acataaattt acacttccta atgggcttac agttttgctt gagccaattg ataatgttgt
    62341 atcggtgtcg tgtggcctgt ggattaaggt gggctcgcgg cacgaaaaag ccaatgaaat
    62401 ggggtatgcc catttcattg agcatatgct tttcaaaggc acagataaat acagtgcaaa
    62461 ggatattgca cgtctggtag acagggttgg cgggcagcac aatgcagcta ccaacaggga
    62521 gtatacctgt tattatatta atgtggtatc ggattattta gaattagctg ttgaaatact
    62581 gtcagatacc tatcttcatt ccacatttga tgaagaagag atagagaaag aaaaaaatgt
    62641 tgtaattgaa gaaatccgta tgtatgaaga tacaccggat gaacacatcc atgatctttt
    62701 tatggatacc atgttttaca acagcccatt ggggcattca atactgggca gctttaatac
    62761 aattagttcc attaatcgag aaagtattgt ttctttttat aaacatcatt atactgataa
    62821 cgaaagtctt tttgttattt caggtaactt caatatagaa gaagcacggc aaattattga
    62881 aaagtatttc tgtaaggatt gtaccgcaac aaataatata agcattcctt cactaccaaa
    62941 gtatgaacgc aaattacgcg tacacctgga taaagacttg gagcaggtac acctgtgtat
    63001 tggtactgat ggcttgagta aagttgatga agacaggtgg gcatattatt tatttaatac
    63061 tattttaggc gggagtatgt catcgcgtct ttttcagaat atacgtgaaa aagaaggatt
    63121 gtgttacagt atttattgtt ttcattcatc gttcatggat acgggtgtgt ttggcattta
    63181 ttgcggtaca gccccttcac agtataacaa actattacaa ttaattgcaa atgagtgccg
    63241 gttaattacg tctcagggaa tttccgaaga ggagttggct gatgcaaaaa gctaccttat
    63301 tgggaatatg gcattaagtc ttgagagtac tgaggttcgc atgagtcata ttgctaaaaa
    63361 tgaaattgtt tttggtcagc aatttacctt caaagaaata gtaagaaata tacaggcagt
    63421 aacaatggaa gattttaggc gcattactca cagaatttta gataatgcac agttttccct
    63481 ggtgacaata ggcaaattgc cgcagaatcc catcaatgag tttcatcttg gaatataaaa
    63541 caactactct gcttgtatca ggtttattta actgaattgt atacgtgaaa aaacaaaaat
    63601 aaaacaattg aatatgctta cattttaaaa taattttaat gtacttgact ttaatgtaaa
    63661 taatcagtat aatcattaat tttgaaatac tatgaaacaa tctcctacat atatattaga
    63721 taggataatt catcaaaaaa aggaagaacg cgaacgtata cgttgtgaat tattagaaaa
    63781 agtacataca gttattggtg agttacattc tgttattgca tttaaagaag catatatttt
    63841 tggttccatt ataaggccat attcatttac tgaaaattca gatattgaca taggttttat
    63901 tggattagat gataaagatt tgtttaaagc aatgtcattt ttatcggcac gtttgggtta
    63961 tgaggtagat gtaatacaga tagagagaca tagattagga gcaactattc tatcggaggg
    64021 attacaatgg aaaaagaaag aatagtgttg ctgaaatcgt caatagaagc tcaaatatat
    64081 ttgatagata aaatctatga caaaattgaa gaaagaaaaa aaaagagagc caagtgggtt
    64141 gttgaaaata ttggctatca gttacacaat ttgtattgtg cttttgaaga tttatttaaa
    64201 ataattgcac gggaatttga aaataatatt gataatggta gcaaatatca ttatgaattg
    64261 ctgaagagaa tggccattgc aatagaaggt atacgaccta aattaatatc tgaagataca
    64321 ttccccttgc ttgataattt acgctcattt cgtcattttt ttagacatgc atattcctat
    64381 gatttagata agcgtaaaat tgatattgtt ttagaagatg cattaaaact aaaagaactc
    64441 tataaacacg acatagagag atttttgaaa gaattagaat cggagtaata ttcatgaaat
    64501 gaaatatatt gctcaaagta aaaagttgaa caaaatgtaa gaatgtgcaa atttcttctt
    64561 cttttagtaa tgggtgttta ttaataactt gacatgaatg aacaaattta ttactcgttt
    64621 tgaatatgtt agatgcactg ataacatcaa aaacgcggct aaagatttta ctcaaatttt
    64681 ttattaatcc ttccactaaa ggatatctgc gtgaattagc cgctcagatg aatgaatcca
    64741 ccaatgcggt ccgtgtggaa cttgatcgcc ttgttgaggc gggctattta gaacgcaacg
    64801 ccaatggcaa taaggtgtac tataatgcga atactggcca tccccttttt aaagatattc
    64861 acaatgttgt gaaaaaatat ttaggtattg atgtgttgat tgacagtgtg cttgccaagt
    64921 tagggaaaat tcatgaagca tatatagtag gtgattatgc caaaggtgtt gatagtggta
    64981 ttattgacgt tgtcattgta ggcaccgttg attattctta cctggcacag ctcattgaaa
    65041 aagctgaagg tattataaag cgaaagatac ggccgctggt actatcacca aaggaaaaaa
    65101 caaaatataa agaaaaactc aatttagaca aagcgctact ggtatggcac aatggaaagt
    65161 aactggtatg cggtatatgt tcgctctcgc catgagttta aagtgcacaa tgctctgtta
    65221 gcaaaaggca ttgagtcgtt tttgccggtt gttgaaagat tgcgtcaatg gaaagacaga
    65281 aaaaagctca ttccttttcc tctgtttcca ggatatctgt ttgttaagct cccggatgat
    65341 aaagtttatt ttttgcacgt tttaaaaatt aatggtgtgg taaaaatatt gggcgatagt
    65401 tccggatatc agcctgttgc tgaagatcag atagtgagta tatataaaat ggttaatagc
    65461 aagcttgcca tagatgagca tccatattta aaagaaggac aaaaggtaaa aatagtcact
    65521 ggtcctcttt caggtgtaca ggggattctg caaagaaaag ggggagaaca atatgttgtt
    65581 gtgtcaattg acattttaca gaaaggagta gcagtaaaag ttaatataca gtcaattgag
    65641 cctgttatat gacttttttc atgaaaaaat gattgtttta tttctacaat tgaagtataa
    65701 aaattatact ttaaatggag gttctatcat gaattacata aaaatgtatg ttactgcatt
    65761 aattcttttt ctgataatcg atgcactatg gttaactgtt atagcaaaaa atatgtacaa
    65821 acaatattta agctatctca tgggtgatgt ggtgtggtgg gctgttatta tattttatgt
    65881 tctatttatt attgggttac aatatttttg tattatacct gcaattaacg cacaatcagc
    65941 aatgcatgcc ttttatgcag gagcattatt tgggcttgta agctatgcaa cgtatgacct
    66001 gacaaaccag gcaaccatta aaggatggcc agtgctcatt acatgtattg atttgttgtg
    66061 gggtgcgttt ataggagcta ctgtcagtgc acttacaaca aaaattgcat tacttttgaa
    66121 gtggcactaa aacgttgaaa caaaacacac gtttttctgg tttttataac gaatgttaaa
    66181 aattaaacat tgctccagca tttactgtat aacgtttaga tgaatgttca ttgtcatgcg
    66241 acatggacag tcgttgtaat tcgccaaaaa cgagaacctg ttctgttatg tgaatacctg
    66301 agcctatggc ccaccagcca ctatttaaaa atctgagttt ttctttttta tcgtatccat
    66361 aatcgtattt taattcactc aaaagtgcaa tgccagctct tacataaggg gcaataatct
    66421 cacttgctgt tatcatgata ataatatcag gcccccatga ttgtttaaca gtaaaatccc
    66481 ttgtatcccc gttgaatgta taatgcaact gttcaccatg gaaataaaat ccaaatccaa
    66541 acatgataac ttcagtatca acattgagat gccccatcac tccgtaatta taccctttga
    66601 gcgaatcata ccgctcttca tagaactcta tatttccgta gggagtatat cctccaataa
    66661 ctgcaatatc tgctattgca aaggtcagtg agggaatagc ggtaatgcat aatgtgaata
    66721 agagtgcaag aatgtttttt ttcatgacta tcctcattat tctgtgtaat gaattgtcag
    66781 gaaccatagg tattgtacgt tttaaaataa tttcatgatg aactgataag ccatctcacc
    66841 tctataacgg tgaaagtata tataaaaagt atatataatt tggtattctt ttaatgaagt
    66901 taagtttatg aatatgtaat aaaatttttc aattcttttt tcatatttaa cctgctattt
    66961 tcttccgaaa atatacatag tctggtatac aaggattgcg tgcaatgaat atcccgtttc
    67021 atgttcccta catcgatgac agcgaccttg aggctgcagt cagcgccatt aaaagtggct
    67081 ggacaacaat gggtccaaaa acaattgagt ttgaggaaag ctttgcccag tatacaggaa
    67141 tgccattttc tgttgcagta agttcgtgta cggctgcgct gcatttggcg ctaaaagtat
    67201 gcaacattgg cccgggtgat gaggtcatag ttcctgttaa tacctttgtt gccacagcag
    67261 aggttgtttg ttactgtggg gctactccgg tgtttgctga tattgacagg gactcccatt
    67321 gcatcagtgt tgattcactg taccggttac tatcgcccaa aaccagagcc attatcccgg
    67381 tacattttgc aggaactccc tgcgaaatgg atgttataga tgatattgct cgtcagcaca
    67441 acctgtatgt cattgaggat gcggcgcatg cattaccatc ctggtacaaa aatagacttg
    67501 ctggtacaat tgggcatgtt ggctgttaca gcttttatgc aaccaagacg cttgcaacgg
    67561 gtgaaggcgg gatgctggta acgcacaatg aagagtgggc caggcgggcc gggagattgc
    67621 gactgcacgg catgaaccgt gacgcatgga ggcgctacga caggggtgga agctggcagt
    67681 atgacatcga agagatgggc tataaatata acatgaccga tattgctgcg gcaatgggtc
    67741 taagtcagct taaaaaagtg gaaatgttgc aaaaaaaacg ggaagcgata gctcaaagat
    67801 ataatgatgc gttttctgaa aatgatgcat taattccgta tgttgtcaac aaaggaattt
    67861 cttcatggca tctgtatccg ctcaagataa atcttgaggc gctaactatt tcacgcgatg
    67921 aatttattgt gaaattggaa gaatgcggca taaaaacaag tgtgcatttt attccgctct
    67981 accgattttc ttattttgta aaacgatatg gcaatcagga atcgatgttt ccagatagcg
    68041 agtgggtgtt tgcacgggag attagtcttc caattttccc ttcaatgaca gatgaacaaa
    68101 ttgattatgt gattgattgc gtgctgtcaa ttgcaaaaca attcaaaagg taattatgaa
    68161 gaaggtactc tatactataa aaccattggc tgttgtcttt tttgttgctg atatgctgtg
    68221 tattatcgct ggatatggta ttgcatcgct attgttttca tattcattgg atgctttcat
    68281 tacacctgcc gtattgcatg catttcttac tatagcagga ttgtatatta cacagagcta
    68341 tcgcatatta tggcggtatg ccacgatgca ggattattat cgtatcattg aagggtttac
    68401 aggcgctaca gtgctattgt tgatatacag cagtgttgtg aatcttgtca gtaatagagt
    68461 tctttttctt gccagtgcag gtggccttgc attactcatg gtctatcgtg tcattatacg
    68521 ggaaacaaag cctcagaaga tcgcattgcc aaaaaagaaa tcaaaaatta ttttaatagc
    68581 tggggcaggt gaagcaggaa gaaccttgct tgctgaattt aaacgtgatg gcagaggtga
    68641 tagtatcata ggatttgttg atgatgatac gcgcaaggtt ggtacattgt tatgtggaaa
    68701 gcctgtgctg gggaccactt atgatatacc ttccctggta gaacagtatg gtgttaaaga
    68761 gtgcattgtg gcaatgccat ctgctccgca aaaggagatt gatcgagtca taaagattat
    68821 tcgctcaagt ggtaatgtca gcatttccat tttaccagcg ctaacaaagc tttttgaaaa
    68881 ggctctgacc ccggagctac gaggaatagg cgttgaggaa ctcattggtc gtgaagaaat
    68941 tgagatagat agtgcggcaa ttgaagcata ttgtaagggt aaaactgtgc ttatcactgg
    69001 agctggcgga agcataggtt ctgaaatttg caggcagcta ttacacttca aagcagggaa
    69061 aatcattgca gttggcagag gcgagcattc aatatataat ctaaaaaata tgcttgatga
    69121 ttatgctaag tactttgaaa aacctgaaat ttattactac atagctgata ttaatgacgc
    69181 tgtgcgcatg caaaaaattt taaccaaaca caaaccacat attgtattcc atgctgctgc
    69241 gcacaaatat gtcgatatca tggagcaaaa tccgcaggaa gcactgcgca ataactgcct
    69301 gggtacacgc acggtactgg aggtagcacg cgctgcaggt gttggtcagt ttgtctgcat
    69361 atcaacggat aaggcggtgc gccccgtaag tgtgatgggc gcaaccaagc gcatggccga
    69421 gattgtaacg ttagcgcaaa acagccccga catgcgcacc tgctgtgtgc ggtttggcaa
    69481 tgttattggc agccgtggtt cggttatacc tctgttttac cagcagattg caaagggtgg
    69541 gcctgtgacc attacccatc cagatatgac gcgctacttt atgagcatac ctgaagccgt
    69601 gatgcttgtt attcatgctg ccatgctttc aaacggtggc gaaatatttg tgcttgatat
    69661 gggaaaacag tataaggtgg aagaggtagc acgcaatttg ataacgcttt tagggtatga
    69721 gcctgatgtc gatattcctg ttgtatatac tggcattcga ccaggggaaa aactgcagga
    69781 agatttatgg aatcatggcg aggagcttgt gccaaccctt cataaaaaaa tatataaaat
    69841 tgcccacaat ggattcgatg cgctatcggt tactaaaata aaagaactaa cacctgacta
    69901 cgtgcagagc ttacatgaag aagaatgcaa acaattaata caagcgatag ttcctgatta
    69961 caggccattt aattaatact tttatctatc cagccaccaa atagtattgc accgtcatca
    70021 tcgtagcata caacagcctg gccgggggtg atgcttgcct gaggtgtttc aaaggttacg
    70081 gtaagaatat cgttttcata cgtggcatgt gctttgaacg gtttttgtgt tgagcgtgtt
    70141 tttacccata cgggagtatt atgggcgata gttactttca taaagtttac ctgtgtggca
    70201 agaagcccct ttgccagaag ttcatccttg cttcccacaa tgatgcgatt atgtatagca
    70261 tcaatagcaa caacgtataa cgggtgttcc caggcaatgc caagcccacg acgctgcccg
    70321 atggtgtagt gatgaattcc tttatgcctg cctaaaactt tgccatcagt tgtgacaata
    70381 tcgccatgag gaggacaaaa gcccaggcgt tgttcaataa agcgtggata atcattatcc
    70441 tgaacaaaac atacttcctg gctttcaggc ctgtgggcag ttgccagtcc cagttcacgt
    70501 gctttttcgc gtatctcacg ttttgtggat tgaccaagtg gaagcataag gtgctttaac
    70561 acatcctgcg aaaggttaaa caaaaaatat gactgatcct tatcagtatc aacgccacgc
    70621 ttgatataat agcggttact tttacatata gttgcataat ggcccgtagc aagcatatca
    70681 aatcctaact ctttggcaaa atttaaaagc tctggaaatt ttatgctggt gttacagtta
    70741 atgcaggggc tgggtgtgcg tcctttaata tattcagcgc aaaatggatc tataacgttt
    70801 tgtgaaaatt catcttcaac gttgacaata taatggggaa tacgtaactg tgtggcaact
    70861 ttttttgcat catgtatgct ttcgggacta cagcatacat cataccgtgg ctggcagcca
    70921 tccatttcag cacacagcaa aattttagcg gtaaggccaa tcacctgatg cccctgttca
    70981 acaagtaatg cagcggtaac tgatgaatca acaccaccac tcattgctac tgctattttc
    71041 ataggtattc ctgttaatat taatgattac tagttagtga taatatacct gaaaagaggc
    71101 caaacagtaa aaaatatttt atcaatgggg aaaattttta tcaggattaa ctactgcgct
    71161 tttttgcggc agaaaagctt ataatatctt tatatcttac ctgcagcaat attttttttc
    71221 atcatccttt tgagcattgc tacacactgg cgatcaatga tgataatatt attctggttc
    71281 attttgtatt tgccaacatc aactgtataa agagattgca tgctttttat agtttcaaca
    71341 atctcttttg ctttttcata atcgtttatt ctgatgcctt tctttttgta ggattctatg
    71401 cgctgcataa gtgatgcact gtcagcccgt agtttacgca acacatattg ctggctggta
    71461 tattcattta ttatttgctg tacatcaaaa ttgtcaacca caggtatggc tgactgcatt
    71521 tcaataatct gctggattgt gttatcgcta aaaccagttt gcaccaattc aaaggctttc
    71581 tgaattgtag acgcatagcg cataagtgcc tgctttgcgt atgtttgcgt attaaagagt
    71641 tttatccctt tttcaagtgt ttcaatgttc attgcaattt ctaggttaca cagtgcaggg
    71701 ttaggtgtat catcttttct ggtgctggca atggtgtata tgttttcttt aattgtgtgc
    71761 agtgtttgca acgttgtggc acgttcatca ttgttttctt ttggtgcatc agtataaaga
    71821 aaagctttca gtacttcaaa tagttgctta acttcaggta ttatttcttt tgaactatcg
    71881 cctaacgatt gtaccagttt ctgaatgtgt gatgcaacat atacaatgtg ttgacaacga
    71941 cttgaatagt taatgtgggg tgcctgtaac agagatagct ggcagtacag cgttgttgcg
    72001 tttgcataat cacgcaaaag ctttgattgt gtttcaaaac gatgtacaac attattaaat
    72061 gcttcttcat tttcaacggc tacgccatct gtggatttca tgattacaag aagcttcttt
    72121 tgttcggcaa tctggttttc tatgggtgtt atgtatgaac gtgctatgga ttcaatgtca
    72181 tcaatctttg tagcatcttt ctcagttact atcgcatccc ttttgttctg tatttcataa
    72241 attgcacggg cacaatccag ttgctgtggt atggttattt gataggggat gccatcagtt
    72301 tgctggtatg tggctgtttt ttctggaaca ttaatgagtg cattttctaa ttgtgcaagc
    72361 agggctttag catgttctgg gttttgccgt atttgttcat catcatactg tgtggtgttg
    72421 cacaatgaag ctatagaggt aatgaagtgt tgctgctgtg taataaaatt ggcatgtgcg
    72481 gaaagctcct gaaagcgtgt gctttgtgaa aggtcatcaa acggtggctg gcatcgttgt
    72541 atgatttgtt ttatatcata ttgttgtgta ctattgttag taaagattgt atcgaactgt
    72601 ctgaaaattg aagaaagtat caattcagtg tgaatgatga tagcatcatt gttgcttatg
    72661 tcagaatgct gtaattcttt ggtaacctgc tcttttgtaa tatcaatgag tgttttatca
    72721 aagcaaagcg atagtgcctg cttacagcaa ttggttatgt gttcctgatg ttcgttgttg
    72781 ttttgtagtg ttgagtcaaa agaacgtgat aactcttgtg ccatggcaat gcgggtgtca
    72841 agatactcaa tcacaaatgg tgcaatgtag ggctgctctt ttgcctgctt ttgttctttt
    72901 atgagtgaat cggcatatcg ctgccagtag ttttcagcaa agagtatgtg tggtataata
    72961 agaaatgtca ttataattgg tgatagtttc atggcaatag ttctcccttc aattaattat
    73021 cgaacgcaat accatgattt attactgtat ttacggtaaa cgatacgtgt acctgcaaac
    73081 ggcaagatgg aaattatgct ttttctatgc tacctttgta atgtggtaat ggtattattc
    73141 aggagtgata tatgactatt gactatcaaa agatagaaga agctgcaaag cttctggaat
    73201 tgggcgatag ggcttcccta cagcaggtta aacatgccta caggaaatta atgcaaaagt
    73261 ggcatcccga ccactgtaaa gaagataaaa agctgtgtga tgaaatgacg aaaaagctca
    73321 ccgaagccta taagctgctt attgagtttt gttatagtta cagaatacca ttgacggaac
    73381 aaaagcttgt tgaagaactg gggaatgaag accctgaagc gttctggaaa agaaaatttg
    73441 gctctgaccc ccactgggga ggtcccgggt atagataaaa aatattttac aacggaagcc
    73501 atattatgta tgtttgttat atactcttag cctgaatact taagatgagt aaggtatgta
    73561 tatttttgag cttatagctg agcaacgtat acgtgaggcc atggagcggg gagagtttga
    73621 caacctgccg gggatgggta aaccattgcc caaagatgaa ctggacaatg tgccgccaga
    73681 actacgtatg gcatacaaaa ttttaaaaaa tgccgggata gttcctgaag aggtggagtt
    73741 gcgcaaagag atttattcat tgcaacagct tatagatgcc tgctatgatg acgaagagat
    73801 gaaaggactt gtaaagcagc tcagtgcaaa acaactacgc tataacatac tcatggaaca
    73861 aaaaggcatc catgtcactg attacgaata tagaaaaaaa atcatggaaa aattatcaaa
    73921 gtaacaatgc tacgcagccg cttaggctgc aggtgtataa ttgcttatcg tcgtatcttc
    73981 ttgttagttt ttgatgggtt cttttgcaaa tgaagatagc gcttgtgagc tacctcggca
    74041 aatcgttgga gtatgtcctt taaatcggaa tgttcccata atgattgtat ggatgaatct
    74101 atttttttta ttgtatagta tggtggtata gtggtaagct caatatcttc ttcggtaata
    74161 gttatttctt ctttttcaaa ggctttttgc agcatttctt ttatggcata tgaaaaatat
    74221 ttttgtacgt caatgatgtc ttctgaggtg cttatttttt ctctgaactg ggggaggaat
    74281 tctttttcta atttactgaa ggaaacctga gtggacatac aaacctcctt gtggtatagt
    74341 ttgtgtatat actatagtat atacaggaat tgaataaaaa tcaagcatca acttcgtaat
    74401 tgagagataa attaatacat cacgcaggga aaatctaaaa gcattgtact ttggcacttt
    74461 cttttgttga cgtctgaata aaagaaatat ataaatcaat aatcgcagtc gctcaagcgc
    74521 ttgagcacct gcgtaatgat tagtaattga ggccaaagcc tgtaataacc aaatatctga
    74581 gcattcaaca aacactgcgt gaggtaaaaa atgtattacg cgggagaagc gcacataagg
    74641 ataaaaaggt ttctggaatg agaaaaggaa taagtatagt acttggttta ctatgtttgc
    74701 tttcatgtgc aaaggatatt acaaaagtaa ctatcgctaa aacatcggtg ccaaaaaatt
    74761 ttaccgctac tgatataaag gtaataagct acaatggcaa tgatgtttcc tgcatgcttt
    74821 ttgcacaccg cttttcacag ggtaatcttg tatactttga gatagttcct gcttttgatg
    74881 ttgaaaaaat tactgtaatg tttaatgaaa aaggtattcc cactacaaaa cgaagctggg
    74941 gattcagagg attttttgcc attgcacctg accaaaagcc tgggacacat aattttacag
    75001 taactatcgg tacaaaaaaa attgatatac ctgtagtggt tgtaagaaca ccattttcag
    75061 tttccaccat acctatggat ttgggcaagt tttctaaaaa agaatatctg gagtcacctg
    75121 aagttttgga gtttatacgc cagtgtactg aaaaaaaaca aaaggcattt gccacacatt
    75181 ataatgatct tatccgtggc agagcgtttc atccccgtga tgtgcatgag gttaattcgc
    75241 cgttctgggc aaaaagagtt tataaacttc atgatagcat ttcaggaaaa acacaaacag
    75301 ttgaacggat tcaccgtggc attgatttaa acggcaatac cggggatccc gtatatgcaa
    75361 tgcttgatgg cattgtggtg cttgctgaaa tgctctttta tgaaggcaat atggttatta
    75421 taaatcacgg caatggcatg ttcagctact atatgcatat ggataagctg tatgttgcaa
    75481 gcggggatac ggttgtggca ggacagctta taggtgctgt gggaagcacc ggcatgtcaa
    75541 caggtccaca tctgcatgtg tcacttgtgg ttgatggagt gcaggtaaat cccatgagtg
    75601 ttgtgatgct gccaatacgg gattaaccaa accgtatttg tatacattct cttccataat
    75661 cttctttaca aaattggtat actattttaa ctatataaaa tagtaatgat gtgctatagt
    75721 ttttatgcga tagttactgc caataaactg gaataagtgt atggcaaaac cagctgaata
    75781 ttttatcaaa tcaaagaatt tagacgaatt cagaaacgac atcattgcct gtgatggcaa
    75841 ctttgatttt gatattgaag agatgattgc attaggaagc gcgtatctgg aacgtttccc
    75901 tgattgcttt tcaaatagaa gctgccagga tgtccagctg ggatatcaat tagcaagaat
    75961 ctgtttagta gaaaaattga ttatagattt cccttctgat gttaaagatg ctttcagaaa
    76021 gatgtttttc agcgctcagg ctgtgaatga gcaaatggag tatttaaagc aaaaattaac
    76081 ctatgatgag ctatcaaact ttgttgacac aatacagaag cgtctggagg agtatcacct
    76141 taaagttgtt agtttgccaa aaggcatgat caaggagcgc tttgtaggtg gaattaccaa
    76201 tctttttaat attgcgtatt tattaaaaat gaatgtagcg caaaaggctc atgaataaag
    76261 agtttttaca cagcataagc catgaattga aatcaccgtt aaactccatt ttaacgctat
    76321 caaaagtgtt acttatgcag gctgacagtc gcctcaccct tgaagaaaaa gagtatttag
    76381 aaattattga acgcaatggt caaaaacttc tggctcttat taatgacctt attgaagtgt
    76441 ttgaaattga tagtggtata caattgtcag tgacggatgt atcgttatct tcagtattgc
    76501 ataatgtgtg tgaacgctat gaaggaatca tgcgcaaaaa ggggctacaa ttgcacatgc
    76561 actgtgatga tattatgctt aatactgatg aagcccgcct gtatcagata tttgaaaatc
    76621 ttattgataa tgcagttaag ttcacagaag aaggaagtat aacaatagca gcatacaaaa
    76681 aagatgggca ctgtgtggtc agtattactg ataccggcat tgggatccgc agtgaatact
    76741 gtgatgcgct tttttcagga tttaccaggg cttcggggtc actgtcgaca aaatatcctg
    76801 gaaccggtct tggtcttacc attgttgcaa ggcttgttgc actgttaggg ggtacagtag
    76861 ctgttgatag ccagttaggc aaggggtcaa catttactgt acggttaccg ttacaaaagg
    76921 aacatgaaga tttgcagcag caacgtaaaa ctattcttgt tattgatgat gatcctgata
    76981 gcatagtggt ggttaaggcc cttttgtcta aattgtacga catagttgaa actcacagcg
    77041 ggcaagaagg attggacctt gcacaggatt taaaaccagc atgtatattg cttgatttat
    77101 cattgcctga tctgcacggg ttgcaggtgg taaaacaact aaaagaaaat gaacaaacca
    77161 gccatatacc ggtgattatt gtatcagctt cttcattaaa agatgaaaga agaaccatta
    77221 tggcatccgg gtgtgatgat tttattcaga aaccttttgc cattgaagaa tttgtgctaa
    77281 aagttcagcg ctgggttaca caatgagcac aattttggtc atagatgata aaaaagataa
    77341 tctggtaagt attaccgcac ttctaaagaa cctgattgcc aattgtgagg tagtcacagc
    77401 acaatccgga aaggaaggta tagaaaaagc agagaagcac aatcctgatg ttattatcct
    77461 tgatataaag atgcccgaga tggatgggta tgaggtatgc cagattttaa aaaataatcc
    77521 aaagacgcgc cacattcctg taatcttttt aaccgctatc catactgata cgcagagcaa
    77581 ggtcaaaggt ttaaaattag gggcagatgc atttttaacc aagcctgttg atcaggctga
    77641 actcattgcg caaattaatg caatgatacg cataaaacat gctgaggata tgcttagaaa
    77701 agaaaaagac ctgcttgagg atatagtgtt acagcgtacc agtgaacttt tagcttcgca
    77761 gatgcgctac aagtcactgt ttaacagtat atctgatatg gttatcattc accgaaggga
    77821 tggaaaaata ttggaggtaa acaataccac ctgtgatcgt ttgggctttt cacatgatgc
    77881 catgatgctt atgaccctgc agcagttaac agctgaaggc gatagcgata gaattaattc
    77941 atacttgcaa tcaattaatg aaacagcaga tccctatgaa acctcatttg ttgacagaaa
    78001 cggcactacc atccctgtgg aaattgtgag cactattatc tattacctaa atgaaaatgc
    78061 tatcttgagt gtggcgcgtg atattactga acgaaagcac attgaacatg tccgtaatca
    78121 attatcaatt gtgcttgata agtctgatga tgcaatcatt ggtgctacac tagatggcaa
    78181 aatagccagc tggaacaaag ctgcgcagca tatttttggc tacgcatttg atgaggtggt
    78241 gggatgtgat ttagctatac tatcgccacc atacaggcct gatgaatttg atcaattgtt
    78301 attaataatc aagaacaatg aggcgataga tcattatgaa acagaggggc tggataagaa
    78361 gggtgcaata cacaatcttt ctatgactat ctcccccctt gttgatgaac acgacagtat
    78421 tattggctgt tcaataattg cacgtgactt taccgatgta aaacgcacac aagaaaaact
    78481 gaaaaaaggg ctggatatca tccagaattt agaggatatt gctccagcat attttatcac
    78541 tttaaatgaa gaaggaaaca tcctcacctg caataaagcc atgttgcagg ctacaggcta
    78601 ttcactgagt caggtgcagg gcaaaacgtg ctgtgacatt tttattccgg atgttgggca
    78661 aaagtcattc cgcaatcaat tgtcaacaat gaagaggacc agagcaacac gcatttttga
    78721 aagccccatc aatacaaaaa gtggtgaaga aaaaattgtt cagtggcatg cacggccagt
    78781 atacagtgat actgaatttg attatttcat gttcattgga atagatataa cggagaaaaa
    78841 gcagcttgaa aaaaagatac ttcaggcaaa cttagaggaa agaaaccgca tagcacgcga
    78901 tttacatgat ggcctggggc agcatcttgc agggataata tttaaaactg agatgctgag
    78961 gcttaaaata aaaaatgatt atcctcagga agctaatgaa attgttgaga tacagaacat
    79021 ggtgcatcaa gctattgagc agacacggct cattgcacga ggtttaagcc cggtggattt
    79081 aagcaccaag ggactgtatt catcactgca ggatatgatt gctgatgtaa ataaaaattt
    79141 cgcaatcaaa accatcctgg aatggaatat aacaagtaac attgacgaag caatagatac
    79201 agtacatgta tattacatta tccgtgaagc attgaacaat gctgtaaagc atgccagtcc
    79261 aaaaaacatt gctattacaa tatctgaaga taatgcatat tattatgtaa agataatgga
    79321 tgatggcaaa ggtatacctg atgaaattga tctggcaaaa ggaatgggta tgcatatcat
    79381 gcagtacagg gcatggatca ttaatgcgtc gtttcaggtg cagaacaaca aaagcggcgg
    79441 aacgctggtg caatgtatta ttcagaagag cactcaaaag gctgttatag acaaaccaaa
    79501 gacaggcaaa aagtatacga ttgttattgt tgatgatcat cctattgtgc ggcagggttt
    79561 acaacagctt atcaatactc agaaaaatct ggaggtgata ggccaggcaa catcagcgga
    79621 tgaggcaata gatgtagtta accgaaaaat ccctgattgc attatagtgg atatttcatt
    79681 gaatgggaca agtggcattg aactggttaa agcattaaaa cagcgtttcc ctaaactgcc
    79741 ggcacttgtg ctttccatgt atgatgaaaa gttatacgct gagcgggcta ttaaagctgg
    79801 tgcccgggga tatctcatga agcaggaagc accggataag attatacagg ccatctatac
    79861 agtcttaaaa ggggacgtgt atctatccag tgaaatgaag gaacagatgg tgcaatcttt
    79921 atcagataag ggtacaatgg acagctctgt agaagcactg acaaaccgtg aatttgagat
    79981 atttcagtta atagggcatg gcttgggtaa caaacacatt gcccagaaat tacatatcag
    80041 tgtaaagact gttgaaaatg tacgggaaaa gattaagatg aaactgcacc tggaaacatc
    80101 ccaggatctt ttgcagtatg cgattgagtg ggtgagagaa aattcgggaa attaggaaaa
    80161 gtaaaagaat tatttgacaa attctttata attcattact tttgatgtaa agtaatgaaa
    80221 attattaatg taaggaacat atgaagacta ttgcaaaaat tacaagaaag gggcaggtta
    80281 ccattcctaa aaaaataagg gaaatgctcg gttcagatat tattgagttt aaggtacaca
    80341 ggaatgatat aatcatcaaa cctgttaaaa gtattggagg ttctctacac aaatattcta
    80401 aacgctatgt gccatttgaa gatatacgag acaccgtatg ggatgaggtc gttagaaaaa
    80461 acaaatgata tactatattg acaccaatgc aattttacgt tatttgttaa aggataatat
    80521 tgagcaattt aataaggcaa atgtcatatt tgaaaaagca agggagggtg acctatacat
    80581 tgttgttctg gaaagtgttt tggtagaatg tatttatgta ttaatgaaat tttataaagt
    80641 tccaaaaaat gaaatcgtta gtaaattaga aacactattg aattataaag gtgtaaaaaa
    80701 ttctgataaa acagagttgt taaaagcttt gcagatattc aaagaaaaaa atattgctat
    80761 tgtagattgt attgtatata gtaaagcaaa acaatctcaa tcaaacatct ttacgtttga
    80821 cgaaaaatta ttaaaattaa aataaacaat tcaatcattg ctgcataagt aaattcccct
    80881 ataacaaaaa taagaaaatt ccctatttac aacatcctga caatgtgcca tattcactat
    80941 agcaattaaa taaggaagca ttttcttagg acagaactgt atgattttga atttcattat
    81001 ggttagtgag gataatctat gagttatatg ccaggtgtat gcaccttttg tggaacagga
    81061 tgtggccact atctcaaagt agatggcaca tctgtacagg gagttttccc atcgcgggta
    81121 catccagtaa gtaaaggcag gttatgtgta cggggatgga atatccatga gttattgggt
    81181 acacaggaac ggattatcaa gccgtatatc aaggatagtg gccaggctaa ttatcaggat
    81241 gctattgcca ggcttgtttc ggcattatca tcgtataaac cggaagagat tgcgttttat
    81301 gcttcgccac gttcgtccaa tgaggacaat tttgcattaa tgaaattggc ccgtttagtt
    81361 tttaagacaa acaatatcag ccttcaagca gaatcaggcc atcgaaatag tttagatgtt
    81421 ctgtataatg gtacgggatt tgcaggtatg ttgggatcgc ttgaagatat acggaaagct
    81481 gaagtgctca ttgtggcagg gatggatata accacacaaa atcccatcat tggcagtgag
    81541 ttacattatg ctgctcgcag tggctcatta cttataacta tcgacagccg catgacaaag
    81601 attgcaaagc tttctaaaaa gtttattaat ataaaaccag gaacaatgcg gatagttttt
    81661 gatgcaattg cgcattatat agataaacaa caatggagcg atacaaaatc ggcacattat
    81721 gaagaataca aaaaatattt agccacagta ccgtttgatt caatagaaaa ggaaacaggt
    81781 gtattgccag ctgagatagc ttttgtagct gagcggctgg taaaggctaa aagtgctgcg
    81841 gtatttttta cctcaggtat ttctggatta agctatgata ctgttgcatc aatttataac
    81901 ctctttgcac ttgcgggcaa gattggtaaa gaaggttgtg gagtaaatcc tattgctggt
    81961 ataaacaatt tacagggtag ctttgatatg ggatgcgctc cggatttact gacaggtttt
    82021 cagagtatta atgatgcaac agctaaaaag aattttgaaa aaaaatgggg gcaggagtta
    82081 cctcaattta aaggaaagac cgttgaacaa cttctggctg acagttcatc accactcaaa
    82141 gctctggtga ttgttgacca tgatgatggc attgtcaaat atgccgatcg tttgaaagca
    82201 ttgccgtttg ttgcctatat tggtgcattt accaataagg ttgcagattt tgctcatgtt
    82261 gtattgccca ttacaacata tattgaaacc gatggtacat ttaccaatgc tgagcggcgt
    82321 gttcagcttt cagtacaaaa atgcgataat gcatttgatg tattgcccat gtggaagctg
    82381 tgtgacacga ttgcccaggc tgccgggaag ccatttaatt accagagtgc atctgaagta
    82441 atggatgaga ttgcttccct cactccttcg tatgaaggta taagctataa actgctttcc
    82501 aaaaagcatg gtgtgcagtg gccgtgcacc aaaaaaaatc atgatggcac aaaatactgt
    82561 gttcctgaaa aagtctcatt tgtactccca caaaaaccgt atgcggcggt gcaggcaaca
    82621 caggagtatc cgtaccttct tatgcttggg aaagcacagc attactggca ccagaataac
    82681 atcatgcata agacctttat tccactgcgt gaatataatg ccacgttgct cttgtatcct
    82741 gacggatatg tggctttagc ttcagaagat gcaaagaaga taggagtgcg tgatcgctgg
    82801 ccagtaaagg ttgtagccga aaaggcaagc atgaaagcga tggttatgat ttccgatgat
    82861 gtggcgccag gtaccgcata tgttccctat tttatcagag agggaattgc atcattttta
    82921 ttagcatata gtgacattgt gtcacagggc gaagaagcaa ctatcccagt acgaattgag
    82981 aaggtgtgat atgtatttaa tgaaaaaaaa tgatgtaaag aaaattgcaa aggccttaga
    83041 agaaaagtat acattgtatg gcccctatgt agatgaggca tttgggcaat gtttctttga
    83101 taaagtaact gcggacaacg ttgacctcaa tgcatccatc cctaccaatc cgccaaagca
    83161 tgtagtgttt cctcatttag aaacgatcat gcagtataag tatgataaaa ctaccaagaa
    83221 ggccgacatt gccatggtgt ttgatgatac cccaaaggca ttgtttggat tgcgttcatg
    83281 cgatttaacg ggcttgcagt gtattgatag gtttttctta ggtcaggaat ttgttgatga
    83341 ggtctatcgc catcaccgaa gccgtatgtt catagtagcc aatacctgtg ttgttccgtt
    83401 caagcaatgt ttttgtgtat gtactgacag tggtccagca gcacaggaag ggtatgattt
    83461 aaatcttacg aatttgggcg atagttacct gctagaagtt ggcagcgaaa agggcaaggc
    83521 atttattgaa tcttttgggt ttgctaaagc aactgaagaa cacctgaaac agaagcagaa
    83581 gcttgtagaa aaatcgatca ccatgtttga ggatattgca actgaatcaa aggcatgggt
    83641 atcacgggtg atgaatcgtg taacaacagg atttataaag gaagaggtat gggagtatat
    83701 tggaaatcag tgtattgagt gtggagcgtg ttcgtatgtg tgtccctcct gtacctgttt
    83761 taacataaat gacgccaata ctatagcagg aaatactgag cgcgtgcgaa catgggattc
    83821 atgttcatac gaaggatatt ccagaatggc tgggggtcat aatccacgga aaccaataga
    83881 agacagaaga aacaaacgat ttttctgtaa actgtcatac tcacaatcaa agaagtatct
    83941 gaggccggga tgtgttggat gcgggcgttg cgcatgggta tgtcctggtg atatagggtt
    84001 acccaatgtt gttacatata tccgaagaga aattcatgaa taggtgatag ctatgagcga
    84061 acagaaaata gtaacagtcc cagagattgc cacaattgaa gaaattaaag atgagattgt
    84121 tgatgtaaaa accttttatc tgaggtttga caataaagaa attgatggca atttcaagtt
    84181 taagtcaggc cagtttatta tgtgtacagt atttggtgca ggtgaatttg cagtatcatt
    84241 gccacccagt cctgaaaatg accgcttcca tataaccgta cgacgaatag ggaaggtaac
    84301 caatgcattg catgatcttc aagttggtga taaagtggga atacgtggac catttggcaa
    84361 tgggtttccg tttgaagaaa taaaaggtaa aaatgttatc tatgtggctg gtggtattgg
    84421 gcttattcct ttgcggtcat caattgtcca tgtattgcag caccgtaatg agtttggccg
    84481 aatcttgctt ttatatgggt cacgttcacc acaggatcta atgtatcagt atatgctgga
    84541 tgagtggcaa aaaattgaag ggtttgaaac atttattaca gttgataatg gcactcccga
    84601 atggaaaggt aacgtgggta ttataacaac cctatttgat aaagtagaga tacctgtgga
    84661 aaatactgta gcatttgtct gtggccctcc ggtaatgttt aatgctgtta taaaagaact
    84721 tatgcagcgt ggaattaagg atgacatgat aatctcaacg ttagagaggc atatgaagtg
    84781 cggcgtggga aagtgtcagc actgtgccat cgggagaacc ctggtgtgca ctgatggccc
    84841 agtatatacc tacaggcaga ttaaaacgtt aggtgagcaa atatgagctg gctacaaaca
    84901 atgtatacta tgttcaaaga tacaacctgc attgttgggg taggaaacta ttatagaagt
    84961 gacgatgcag ttggtctgta cattgttgat gctctggcag ctaaagttgt gcaacctcat
    85021 gtagtcataa ttaatgtaga agatgttatc gaaagctacg tgtttgcaat tgctgaatca
    85081 aattgtaaaa atgtgttgat tatcgatgct atagaagcgg acgcagaaga aggcacggtt
    85141 gtttttggca aactcagtga ttttgttgaa acgggattag atgtatcaac gcataaattg
    85201 gcattacgga tgtcatgcaa aatactggaa gcatcgggca aggatgtata catgctgggg
    85261 atagttatca agcataatga ttttgggaag ggactatcgc ccataataca gaataacgtt
    85321 gattcaattg ttagtatggt tcaatcctca attatgagca accaaaagga gtgtgtgtat
    85381 gaacattgat ctttatggaa tgttattaca ggcttttgct atattgcttg gtgcaagtat
    85441 tattgtacca ttgatagctt caaagagaaa attagcaggc tggataaaca ccattgcagt
    85501 ggcaattgca gcaggattgt tgatgtatgt tgcatatatg gcaatctttg tacaacaggc
    85561 tgcagaaaca caggcaatat attttggtag tgtgccagtc tattttctta tagatgcatt
    85621 ttcaggattc tttattgcca tcatttcgtt tatggcagtg atgagcgcac tgtattctat
    85681 tgactatatg gaacattata aggactactc gcttgtgcca tactacataa acttcccgct
    85741 attcatactg gggatgattg ggatagtaac cgttgatgat ttgagtattg gattcacggt
    85801 agcatggcag cttatgacaa tagcatcgta ttttctgatt cgatttgaat ataaagaaaa
    85861 acagaatgtg cgcaacgcaa ataaatattt aatccttatg gaattggcat ggctttttat
    85921 tgttgttggt gcattcctca tcaatggttc ggctatgggt gattcaatcc atacattaac
    85981 acagaagctt ggtacatcag gagggatgat cccggtagtg tatgccctgc ttttacttgg
    86041 tttcagcttt aaagctgggg tattcccgtt tggtcagttg tggttgcctg atgcacactc
    86101 aattgcaccg tctcctgtta gtgcactctt gagtggcgtt atgattaaaa ccggcattta
    86161 tgggatagtc agaacatttt tctggatgat accgcatgaa ggtcatactg ttaatcctct
    86221 tgtatgggga agtataattg cgacgtttgg tgtagtgacg ttgtttattg gcacctccca
    86281 gtctttgaag cagagtgatg caaaacgcct gcttgcttac agctccattg gccagattgg
    86341 ttacattgtt ctggctattg gagctggtct gtatatgtat cacagcagca atatgtttgt
    86401 acagatgctg tcatttctgg ttataattgg gatgctgtat catgtactca accatgcaat
    86461 ctttaaagga cttctcttcc tcaccgcagg tagcatattg tatgaaactg gtacaaagga
    86521 tctcaataaa ttaggcggtt tgcttaaatt catgcctgtc agtgcggtga ttgcaggaat
    86581 tgcttcactg tctatagcag gtgttcctcc tttcagtgga tttgcaagca agtggacaat
    86641 aatctctgct acactacttg gtggcagcca ggttttcttc cttgccctct ttggcatcat
    86701 tgctctgttt accagcgcat tgacactggc atgttatgta aaattctttg gtatgtcatt
    86761 tgcttcaaca ggtgtggaat ggaatgttga acacaaacca caggaagtat cctggaagat
    86821 gcttgtgcca aagatcattc ttggattgtt gtgtatagtt cagggtatag tccctttcat
    86881 atactatgaa acgataatga atattttcag gtcctcaact ggatccatag tacagaattc
    86941 atttaacgaa ctatcggcac acaatgctgt gtttacaaat gcattaggtg tgactataaa
    87001 tgttcctggt atgcagtacg ctaatgccat agctgttccg cttgttgtga tagttattgt
    87061 gatcattgga tttgccgttg catatatgct gaaaaagagt gctggttctc aggaaaaagt
    87121 tatgccaaca tggttgtgtg ggtatcagga ccttaataat aataacagat ataaggacag
    87181 acatatgttt gcagcgttaa agtccatgct gtggtggaca ggtggtaatg tgaaataata
    87241 caacatttag gaataagagg taagatatgc catcgataga ccaagtaaaa aaagatttaa
    87301 aagaaaagtt caaaagcact atcaagggct ttgaaacacc acttgaaaca cggttatttg
    87361 ttgatgtgga caaaaaggat atacgggcca tatgcaatgc agttattgat atgggtgcac
    87421 ggtatatagt gagtatagga tatgataata tagcccgtga taatacattg gggttagtac
    87481 atactttcag ttttgataaa gacaacatct tctgttgtgt caggacaaaa gtacctgctg
    87541 acaaaccggt gcttgattca attactcctg atattcccaa tgcagggtgg tcagagcgtg
    87601 agtacatgga cttacttggg atgcagtttg aaggacatcc aaagccaaaa cggctgatac
    87661 ttgccgatga ctggcctaaa ggcgtctatc cactgcgtaa ggaagtccca tggaatttaa
    87721 tgcctcccgc agctgaagat gtggcatatc agttagacga agcaccagaa gggtgtacca
    87781 ccgttcccat tggtcccttc cacccaacat tgcatgaacc ggcacatttt gcagtgtacg
    87841 ttgacggtga aacaataaaa ggatgtgatt accgtggttt tatgacacat cgtggcattg
    87901 agaaattgtg taccacacag gtaacctata atgaagtacc atttattgca gagcgtattt
    87961 gtggtatctg tggatcagtt catgccacat catattcaca atctgttgag cttgctgcag
    88021 gaataaagat ttcccggaag gcagagtaca tacgtacatt tatgctggag cttgaaaggg
    88081 ttcattccca tttgctgtgg ttgggtgttg ctggccacct tattggattt gatacggtgt
    88141 tcatgcattc atggcgtgtt cgtgaaccgg taatgtggct tgcagaaaag attaccggta
    88201 accgcaaaac gtatggaatg ataatcattg gtggcgtacg ccgtgatatt ttgccgcaca
    88261 aagaagatat actaaatgta atggataaag tggaaaaaga acttattgca attaaaaatg
    88321 caattgtagg cgataccgct atccaccgga gaacaaaggg cgtggggtat gtttcaaagg
    88381 aagatacaat aaagtggagt ttagttgggc ctgttgcccg cgcacggggt gtggatattg
    88441 atacacgacg cgaccatccc tatgcagcat acgatgcagt acaatttgat gtacccgttg
    88501 ttgatacctg cgatgtatgg ggaactgttc tggtacgtat ccttgaaaca tttgaatcaa
    88561 taaagattat cagacaggtt ttacgggaca tgcctgttga tgaaccattg ttatatgaat
    88621 ttgatgagcc cataaagcca ctggttcatt caatatctgc tgttgaagca ccacgtggtg
    88681 aagtagttca ttaccttatt actggtgaag agaacagggc agatcgttgg cgagtacgtg
    88741 cccccacgta tcccaactta cagggcgtac cattaatgtt actgaataat cagttggccg
    88801 atgcaccaat aatcattggc agtatagatc cgtgtttctc gtgtactgat agaatggcac
    88861 tggttgatct aaaaactggc agacaatcaa tacttaccaa tgaagagctt gaacgtctgt
    88921 cacgaagcaa aaaagtataa ggcaggtggc atatggatat tctcttgata gtacttaata
    88981 ttgtagtgtt tttggggttg gcacccttat atgaaggtat aatacgaaag ctgactgcaa
    89041 aagtacagtc acgacaggga ccacctatct atcagccgta tctggattta ctgaagcttt
    89101 tgggtaagga taatttagta agcgataact gggcattcaa gctggcacca atgatggctt
    89161 ttgcagcagt aacagcagtg gttacaatag ttcctattgg atttaaagca aactatttaa
    89221 cacactatgc tgatgttatt actattgtat atttactgac attagctggc atctccattt
    89281 tgttgggtgc attatcaagc aagaatgcgt atgcaatgat tggtgcaagc cgtgagatga
    89341 taaccatgat tatggtggaa ccaatacttg ccatgacctt tatactgggt gctgtcagga
    89401 tgaaaagcct ggctatcgat gccagtatgt tcagtattct ttccactggc tattcatggt
    89461 cggtggtact catgcttgta gtatacttaa tggcattgca ggcatttgtt gcccgtcagc
    89521 catttgatat ttctgaagct gaagttgaaa tactggaagg accgtttatc gaatacagtg
    89581 gtcctaacta tgcactgttt aaatattcaa tgatgttaaa gcagatgttt tatgctgtac
    89641 tttttgtaac tgtatttatg ccattcctcc atacagggct atttgtggct gaccttgttt
    89701 tgcagctttt agcagttgcg gttatttttg tgctgattgc attactggga tccaccaacc
    89761 ctaggttacg tattgaccag gcagtaaaat actatgcagt gctcattgtc atatcattgt
    89821 gtaccattgc attaagtgtt tatggaatat aaatcttaac gttaaggaga atgatcgtgt
    89881 ggcttacaag taaaataaaa cagctatttg tagtaactaa gcccggtacg gtaacattgc
    89941 cctatccttt tgaaccgcgc cctgtgccgg aaaattacag ggggcagcca tactgggatc
    90001 atcataaatg tataggatgt ggcgggtgtg ccaatcactg tcctgcccgt accatacttg
    90061 tgcgcgatct ttgcaatgag ctcagggtca tgatgtatga tggttcacgc tgtacctatt
    90121 gcggacggtg tgcagacctg tgccctgaaa aagctataac aatgactcca cagtatgagt
    90181 tggcaaccga tgacaaacgt gacattaatg aaacaatgga attatttatg ttaacatgcc
    90241 agcgatgtgg aagatgtttt gatcatgaaa taaagaatgc aattgataaa ctggcatatc
    90301 tgggataccg gtatgataat ttagagatga gagcagttat tcctgtttct tcgcagacgt
    90361 ttgacaaaga attgtatgaa aaaactaaga cctataaaag gcctgaaaag ataggagaat
    90421 agccatgttg ggtagattag caaagaaggc atttccaaag tcattgtggg tgtatcactg
    90481 caattctggt gcgtgcaatg ggtgtgatat agagatatta aatgtattaa ctccttacta
    90541 tgatgtggaa cggtttggca tcaaacttgt tgcatcaccc aggcatgctg atgtgatgct
    90601 tatttcaggt gcagttacca ggccaacatt ccccctagta aaaaaagcgt atgatgcaat
    90661 gccatcacca aaacttgtat ttgggattgg atcatgtgca acaggaggcg gttgctggtt
    90721 tgatacttat aatgtaacag gtggtgctga taaggcaata ccggttaact attttattcc
    90781 tgggtgtcct ccaaggcctg aagcaattat ttatggtgtt gcattggcgt taggtctggt
    90841 ggataagaag gctgctccta ttgaattgaa gcagatagaa ttcccaatag atatctatca
    90901 gcgaacgaag gcatgggaag aaagaaatgt catttatcag ctgctctatg attaaaattt
    90961 ggtagatgga gtatacacaa tggcaaaaaa aatattatta gttgatgacg atagagacct
    91021 ggttgaaagc ctgaagcagg ctctgacaat gaatggctat gaggtggaag ttgcctatag
    91081 tggtgctgaa ggcttgaaaa agttactatc gtatcatcct gatcttatga ttttagacgt
    91141 catgatggaa actgatactg caggttttga gatatcatat cagatacgaa gcaacaggga
    91201 agattcaaaa tataaggatg taaaaaatgt accaatcatt atgctgacag caataaatca
    91261 ggtgactaat tcacggtttt cactgaatga agaacaatca tttctacccg ggataaatga
    91321 ttttttgaca aaaccggtac gcatagatga attactggaa aagatcaaaa aatatgtatg
    91381 agtttatcgt gatgtgcata tagtatcaca acaacctgtt tacaatcgca aagccggaat
    91441 gtcaggatca gatgttccgg ctattttcta cttttgcttg tacatgaata aaaaggttgc
    91501 aaaatatatg caataagatt tattatatgt aatacaatta aatgaatgat tacataacaa
    91561 tatgagaaag cgatttgcca cacagatacg gtttaaatta tttattagca tactttccat
    91621 tgtgcttcta tcaggtattg cctcaacctt tgttggcata cggacaattg aaaaacatgt
    91681 tgttgggcag gcttttgata ctgtattgaa agatttagaa acagctgatt ttatttacaa
    91741 tagcaggatt gctcttaaaa gtaaaatgct caagcatatt gcgctgttag agtatattca
    91801 attggcaatt atacataata accgccaggt gttaattaat aaatgcaaag aagtgcagaa
    91861 ggaattggga attgatattg tcaatattac agatgctcag gggaaagtga ttgcccgggc
    91921 aaataattat tcatttggtg atgatgtttc cgatgataat catatttcgt atgtattaaa
    91981 aaataaggca ccgtgtgaag gaacagatat tgtcacccat aaacatcttg agcgggaagg
    92041 agaagcatta gctaaaaaag taattattga tattgtacca actcctaagt caagaaaagt
    92101 tgaacggaca aaggttgttg atgctctagc aatacgggcc accatcccta tattttataa
    92161 taataatttt attggtatta tttatggcgt gaggatttta aataattata acgggatagt
    92221 agaccagata aaagaactgg tttttaaaga tgaaaagatt gggggactgg aggttggtac
    92281 ttcaacaata ttcctggatg atgtcagagt gtgcaccaat gtaaaaaaac ctgatggttc
    92341 acgagcaatt ggtacactgc tttccgagga agtatacaat aaggtcatat tgcagggaaa
    92401 ggtctggtgg gacaaggcat ttgttgttaa taactggtat atatctgcgt ataagccact
    92461 gttaaatgta aacaataaac ccattggtat tctttatgtt gggatacttg aaaaaaaatt
    92521 tgatgagatt aagacaagtt caactgtcaa ttttgtagtc attacaattt taattgcaat
    92581 ggttgcggtt gggatttctc tgtatctttt aaacatgatt atccagccgg tgcgaacact
    92641 ggttaaagca tctgagtcag ttattaatgg aaactatgat gcacacattg agatagaaag
    92701 tgaagatgag atagggtatt tgtgttatac atttaaccag atgattgatg caataaaaga
    92761 aagagataga aagctaaaag aacaaactca aaaccagata ttgcaatctg aaaaattagc
    92821 ttcacttggc aggttggcat cgggaattgc tcatgaaata aataaccccc ttacggccat
    92881 tcttacctac agttcccttt tgctggaaga tttgaaggat actgattttg aggaagactt
    92941 aagagttatt attaaagaaa cgtttcgatg cagggatatt gtacgcaaca ttttagattt
    93001 tgcccgttca acaaaacctg atataaaacg gactaatatt aataatctta taaatgaagt
    93061 tcttctgatt cttgagaagc atgttaactt tcacaacatt gttataaata aagaactgga
    93121 tcctgatgtg cctgatatgt atatagatga aaatcagttt aaatcggtta ttaataatct
    93181 ggctatgaac gcagcggatg caatgccaca gggaggcacg ctgacagtga aaacactgta
    93241 taatttacat aatgatagta tcaccataat tgtttctgat actggaacag gaatcaagga
    93301 ggagcattta gataaaattt ttgatccgtt ctttactaca aaggaacagg gaaagggtac
    93361 tggacttggc cttgctgtaa cctatggtat tatcaagcga tactatggta atattgatgt
    93421 gcaaagtgag atagggaagg gaacaatttt taccattacg ttcccattgg ccaaaaataa
    93481 agaagaatat gcagtatttc aaaaagcatg aaaggtgtac gttatgaaag atatacttgc
    93541 aagaattctg attgtagatg atgaggatat agtactcaaa agctgtctca gagtgctgca
    93601 aaaactggat tatgaaattg acaccgcata ttcagggcaa actgcgctgg ataatcttga
    93661 aaaaaagaaa tatgatattg ttgtcaccga tctcatgatg ccgggtatgg atggaatgca
    93721 gcttctggaa gaaataaaaa aacggtatcc tgatgtaatt gtcattatat ttacaggtta
    93781 tgcaacagtt gatacaacac gacaggcatt gaaggcaggg gcatttgatt atattcccaa
    93841 gccgttcact cctgatgagt tgcgcaatgt tatagacaat gcagttaaaa gcttaaaaaa
    93901 taaaaaataa tagcgagtga aatcagtcaa aagttattat tatttcgtta tgcatattcc
    93961 tgaattaatc tgtcatagtg gcggtttttt tcttcatgca attccataag ctcttcaagt
    94021 tcatcataca agctgcttaa tgttttttca agtttattaa tttcaatgga taaacgttga
    94081 atttcactgc tcttctgtga ttgtgatgca atgataatat cagcattaca ttgttttaaa
    94141 aggttgtcat actgcataat ttcattttca atgtactcaa tgcgctgctg caatggtctg
    94201 cattcacttt gcttttgctg caacaattgt gagcgtatcc ttcgtaattc ttttttattg
    94261 attttgcttt caggctgatc agtatcacct gttcttgtac tttctgattc agaaacccag
    94321 cccacctttt ccagaaagcg ttcataatcg ccttcaaaaa tgcttatagt attattttga
    94381 aaaatgatta atcgctgtgc gatagctcta aggaataatt cattatgcgt aacaagtatc
    94441 actgcacctt caaagctgtc aatagccgta agcaatgcat cgcaggcttc catatctaag
    94501 tgattcgatg gctcatccag aataagaaga tttataggct taaccagaat ttttcccaaa
    94561 agaacccggc tcttttcacc gccagataga accgatacag gtttcaatgc gtcatcacct
    94621 tcaaaaagaa gtgagccgca tatagtacgt gcacgctgtt tgttgttatc cgggtcgctg
    94681 taggaaatct catcttcaat agtagcattg gggtttaatg ttgaaatatt agtttgttca
    94741 aagtatcccg tttcaacgcc taatgccctg gtgatagttc ctgattgtgg ttgcaggctg
    94801 ccagccaaaa gtttacacag tgtggtcttg cctttaccat ttggacctat tatacagatt
    94861 ctatcattgg cagctactga gaatgaaaga ttgtgtatta aaggtgtttc atcgtagcca
    94921 aatgaaatag aatcaacctg taaaacatat tttgcattta tgggcttata cctgaattca
    94981 aaatcaagat tttttaatgg tacaagcttt tctcttttct gcatcttctc tatggtttta
    95041 attcgggact gtaccaggtt ggcaagccgt gcttttgccc ggaatcgtga aataaattct
    95101 tctatttctt tttttctgcg ctcgtcgttg atgcgggttt tttcatatat ttcttcttca
    95161 agcgcaattt gctcatagaa ttgctgggta ttgcccttta ttttttttat tcggttgcgg
    95221 tgaatcccaa gagtgtgggt gacaatagaa tccatgaatc ctctgtcatg cgtaataaac
    95281 agaagttcac ctggccagtt ctgtaaaaag cgggctaacc agcgaataga ggtaatatca
    95341 agataattgg ttggctcatc cagtagcagc atatcagggt tcagtatcag tgtttttgca
    95401 agctgcaggc gcacctgaaa tccccctgaa agctcatgca ccctttttag catgtcactg
    95461 gtggtaaatc cgagccctga taaaatcttt tcagcacgcc agtattcatg ttgctcagat
    95521 tcaggcaaaa gcgataatgc ttcatcgcgg actaccggtt ttgtaaaata aagctgctgg
    95581 gacatgtacc caatagtata atggcgaggg atagacacac tgccttcatc aggctgttca
    95641 atgccggcaa tgattttcag caacgttgtc tttcccagcc cgttgcggcc aacaagcccc
    95701 accttttctt tactgcctat tgtaaaggta acatcttcaa aaagaagtgt gctgccaaat
    95761 cgtttagcta aattttgaac tgcaatcatt ctgtaatatc taagctagta aattcaatac
    95821 cgtactcttt cccaaactga tttaatgatt caagacattt ttgtttcacg acatcaacat
    95881 ttttttcaaa ttcctgtttt gtataaccag cagcaagttt tgacagttcc tgcaatgctt
    95941 tattgctcat ttcctggtct aatttctcaa aggtaaaaaa aggtgtatag gggtcccata
    96001 acaggaatgt tgtcctgact gttaatcttt gtttggaact atcgccaaat tgtatttcct
    96061 gtgataccgg cacgccctcc cttttttcaa cgggaacaat gcgcgacgaa acaaatgggc
    96121 taataatgtg taatcccggc tgtaacgttt tatgatattt aagctttttt tcaattatgc
    96181 agatagaatc acgagggaca atctttatca ttctctttaa aagaattaaa ataataatga
    96241 taataatact gtacacatac accatagtgt tacctccaca aaaataatac atactgtata
    96301 tattgtgtta caggtttata atattaccag aacattataa aagtatatgt acaccagagt
    96361 ataaattatt tcaacaaaag ttttaattat tttagcgata gttcatacta tgtaaaatag
    96421 tatttgatta tacccgcaag accaatatga gcatgcaaat atttactgac ctgtaaaaat
    96481 acacttgaaa aaagtgtttc agggatatac aatgtttttt gttacaggat cgctactatg
    96541 gatgttgaac caatacgaat tcttgttctg gatgatgacc ccttcattgg caagatgctt
    96601 gccacaatgt tagctggcta taatgtgaaa aatacaacat cgtttggcca gttccagcag
    96661 gtcatagagg aatttaaccc agatattgca attatagatt tacatttgcc agacgcacat
    96721 gggcttgatg tgtgtaaatg gatcagaagc cagaatcagt atgaggatgt tattttgtat
    96781 atattgaccg ctacaaacga tgaacaaaca ctcatcagag catacagcat tggcgttatg
    96841 gattatatca taaagccttt taacaagttt attctatttt caaaattacg ccgatgtgct
    96901 ctgatgattg agatcaaacg taagcttgag cattctttgg aatatcaaaa agatataaaa
    96961 aaacgattat tacagctgaa tgattttatt aaaaagtgca ttaaaattga tgatatagat
    97021 actcttgtag cattattcct ccatattaaa tatattattc cgcttgatgg attatttata
    97081 tttaacaaac ttacaacagg gaaatatacg gtattgaaat taatttcgca tgaatcctgg
    97141 caatcgctca ttcctaagct tcacaaagtt attggtcaaa atactatcga accggtctct
    97201 tttttctttg aatccactac taaagtatat attgccagca tatcattgca acgatatgga
    97261 tatgtatttt ttgtcagtaa tcttccctac actcctgatg aaagaaatct tattggatta
    97321 ttagcagaaa tcataagagt gataataagc agaactgaga tttatgaata tgttcagaag
    97381 caaaataaaa tatatcgtga tgaaatatca aaagtgaggc agatacaggt tgcgcaattg
    97441 ccaaaattca gaaatattaa tggatatgaa atagcatcaa catttttgcc agcggaagac
    97501 atttcaggtg atttttttga tgggttttat gttgataagg atgtttttca gattgtattg
    97561 tgtgatgtgt caggccatgg gatagcatca tcgtatattg gaaatgcaat gagaacacta
    97621 atccgcacat tcagttttaa tgaacaatcc ccggctaaag tgttgtataa agtaaaccgg
    97681 cagatgatag ctgatggtaa aggattatat tactttggaa cagcagtaat ctgtagacta
    97741 tatccggatg gcacaatatt gtatacatca gctgggcatc ctccactcta ttattataac
    97801 tataatgccc acaaaattga agagttaccc aatactggtg gacttttggg tgtttttgaa
    97861 gacatggctt accaggatgc tacaattatg ttgcaaagtg gggatatgtt gtttttatat
    97921 actgatgggt tgaccgaatc catgaatcct gccttaaaaa ctctgtatgg agatcaacga
    97981 ttaaaagata cgatatttat aaacgcacat gaaaacccgg tggaattatt acactccata
    98041 tgtgggagta tgtatgagtt tactgagtat gcccctttag aagatgatgt aacattaatt
    98101 tgcataaaaa aaatttaact tttcctgaaa gcctgtaatc tgaaatcatc gataattcct
    98161 gagatgtaat tctgtacctt ttttattgca gattgtggtg tgtcgttgtt aaaaaaagtg
    98221 attatttgtt tccgtttgtc ctcttgcaca tatagtctgg caaatgagat gctttgggta
    98281 ttgctgtaaa taggcaatac agtatcaagc cattgatgaa tttcatgtag aagaaatgca
    98341 cattctttga ggtattcatt tttgtctttt tctatttcta tatacttcac ggatggcctt
    98401 tgtaaaagtg ctatatgatc ttctattctt ttagaattgc gtgtggtact gtgggcacat
    98461 aaatactgaa gtcgctgttc aaattcataa agttttgtac gatcttcaag tattatccac
    98521 aaaaccggaa aataatactc aagcaattga taacatgcac cgtacatatg cattatttca
    98581 aaatgtgctt tggggtggaa ttcaatctta gggtattttt taatttttgc agttaattct
    98641 tcaatgaggg tatctacttt tttactgaaa ctgctttcac tttttttctc ctcataacgc
    98701 tttcgtattg tttcaaaatg ggctatttca gctataataa aagcttcaag atcaattttg
    98761 ctccttaaag cagcctgtag tcgttcttca aacgcttcct tgttaaacca gcgagggcta
    98821 tatttatgtg catattcatc atatttattc cgtagttttg taattaaagt ttctatctca
    98881 ttatcctgta acatgtgcat tactcacttt agtttgtgat tatacgcttc aggtagttta
    98941 cgatctgtat atcattacac taattcatat atccttgcaa gcatcatttg agaataaata
    99001 gtgaaaattg atgataaaat gtcgattatt atacagaagg aatagttgtg ccctcatttt
    99061 cataaatttt tagtaaagga tgatcagtgg ctataattca aattcctgat caatgtaagg
    99121 accctttcag ctttgaaacg gttatttcat tataccagca cattatcaat caaaaagctg
    99181 aagggcttga tttgtcccat gtgacgtttg ttgacccata ttcattatta tgcttgcttc
    99241 ttattggtcg caattacctg cgattaacag ggaacaagct gactttgcac cgcatcccag
    99301 cacaaatatt gcagtatatg acaaggatgg atttctttgc acataatata gttgattgtg
    99361 atgatacact ggagaagcac aaacatctga aacgaagcac aacgtcctca cggttgcttg
    99421 aggttattga aataccgggg aaagaaaagg aaagtgtcag ggttattgca aaagttatag
    99481 ccacgttcag aaagcgtgct cttgttattt tgaaacactg gtttacatta accatggtgg
    99541 attatcttgt taccgtcatt tcagaaatat gccagaatat ttttgaacac agtttggatt
    99601 cagggtttat tgccattcag agctatatgt atttaaacga gcgcatattc cgaatagcta
    99661 ttgttgattc aggtattgga ataccgggga catttgaata tatgcccgaa attaaaacaa
    99721 tgtccaatgc taagttgctg caaactgttt taacgactcc actttcaagt aaacgtcgat
    99781 atggatatgg tttgtgccag gttaatgcca ttatggaaca gctgaaaggt tcaatttttc
    99841 ttcgctcaga gaatgcatac atcagtgtat tgcatcatcg ccgttcggca aaagggagtt
    99901 atatatttct aaaagatgac tgcccttctt tccctggtac tcagttttca atcacactta
    99961 ttggatgaaa tgatggtaat tgaaattaaa aaattagcaa catctgttgt tgtaactcgc
   100021 aaaagcactg acttatttac gcggtatgtt ggcaagttgt tatttcaaaa aattcaagaa
   100081 aagctaaaac atctagctga acatgaagtt gtaatcattg attttgatgg catacgctca
   100141 gttgatgctt cgtttgttga tgagtgcatt gtaccgttat tagaactatc gcaaacaaat
   100201 gcttttccgt tttatatcaa gttagtcaat ataaccgata atgttgaata tattgtcaat
   100261 caggttattg gaatgactca tgatcaaaag cggtatattg ttatgactga tagattatgt
   100321 aaaaacggat gccatgaatt gggttctata tccgaaatgg aaaaggatat tatagaatac
   100381 tgtgtaataa acaaacaagc aaccagcgct gatattgctt catttatgca tgtttcagaa
   100441 gccgaagccc gggatagtct tttgcgtttg tatgaaatac gtgctgtaag aaagatacat
   100501 gatgatactg tatttcaggc tatttagtaa aatacttgat aaataaataa ctttctgtat
   100561 aattgaataa attatgatta tgccagtatt aatataatca ggtaatttct acagtatcat
   100621 gaaacagatt attaaaaaga aagaaagtga agatgattca ttatttgggc ttttagcttc
   100681 atataagaat ctttacagct atatgctagt tcttgaatct caggaaggta ctattgctat
   100741 tattatcgca aaaaacccat catcgtttga tgaattgata aaaactaaat accagcaata
   100801 caagccagtc agaagaataa agatgggcgt ttccaggttg tttgtaaaat atatatcgcc
   100861 agaatatatt ataaagtttt atggcccact tttgggctta aaaattccag aaaataaagt
   100921 acatattatt aataatttag aaaatggtta tttcatactg gaggttcctt ctcattataa
   100981 ggagcaggtt actcagtgta taaagcgtat tattggatta accccccaga aaaaatatga
   101041 aataattgaa aaaagtttag ctgatcaaga ggttgaagta taaatgccag ataataccca
   101101 atatgattca ttcagtgatc agacccagct tataaacaaa gcgttaaaat atacaaatgg
   101161 gaatctggaa aaagcccgcc agatggctgc aggaatgttg caggatgttg ctgttattaa
   101221 gggaaggttt agagcaggca aagtaggtgc tttttatatt tttctgaatg ttgaatataa
   101281 ttatattatt aatattaact cactggtaac ctcatatagt gatatttaca ataagattcg
   101341 tatttttgat gcatggaagc aattttataa tttatttggt gatattgtag gtgatttagg
   101401 tggagctact gaggattcgt ataaattcac aaatcattta gctgatgcaa tagaagggta
   101461 tgatatctat gaaccagcca aagctcaaaa tatacaggtt gtatcagaac tatttgagga
   101521 aattataggt aaatattttt cacagaacac agaatgccag attgaaatag ataaaacctc
   101581 ttcattaacc ctggaaattg aaaatatacc aatggaattg ccaggaaaac aggagcaaaa
   101641 gcaagaggaa cttgaccccg atgagataaa aatgcgtgaa atagaatcac aggtagaata
   101701 tgttattcca ggtacagtta ttgtgtctcc tgtaaaggga aagtatatca atgatattaa
   101761 agcaggtgaa aagattactg taatgctcag tggcaaagac ccggtatccg ataagattgc
   101821 acgcatgttt aacgcaataa ccactgatgg gcaatacctg ccggtaaaag ctcggattaa
   101881 agaaaaggtc tcgcttgcaa caggtggctt tgcaatatat gcgcttgttg caaaaaatgt
   101941 actggttaaa ataatagaag aagaaaatgt aaagattgaa acagataagc aggaacagaa
   102001 aaaggaagaa agtaatgaaa atatgctatt tgtttatatc gcacttcttt tgggattgtt
   102061 gatcattaca ggtttcattg tttttgcttt gttgtaaatg aaactattgc agatcaatat
   102121 tatatttctt cattttagct ttcagtgtgt tacgtgcaat atccagaacc cggctggttt
   102181 tctgaagatt ccagttggtt aattccaggg tatggaggat gtagtcgcgt tcaattccat
   102241 caataacttc ctgtaatgtt ttatagtcag gtggtaaata tgatgatttt ttccgccctt
   102301 ttctggtggt attttttata ataatttttt cctttatagc tgccatatgt aactccttag
   102361 taaaccttga aagatgtcag cgttttctat acataccatg aaatatgatg tatagaaatc
   102421 atcacccgat acacatgctt ataccaattt caacataaat catcactggt aattttttca
   102481 atacattttt ttatattttt attaaaatat acttttttat ataaattgaa tatgcgtggt
   102541 aaatagtaat aaaacaaaaa aataaatatt acataaacat attaataggc aaaattagtg
   102601 atcatgaagt taaagaagat atgataatag gatacttggt gtatggttgt attaactgat
   102661 gtattaagtg aaatcaatta catctattaa aataattgtc atgtcgtcat aaagattttg
   102721 ttcatttgct gcccatgtat atatatcttt ttttatttgt aagcatgtgt cttcaggtaa
   102781 catatgtaga gtttgaatca ataagttaaa aaaattatct tccccataca tttcacccga
   102841 aggattacac gtttcgatga ggccatcagt ataaagaata attcgctgat catgatctaa
   102901 ttggtgaatg atattgttgc agtttagtct atcaaatgca ccaataattc ttccctttgt
   102961 aggtaattca ttatagtctt ttgtttttcg gttaataatt atacaacggg gatgccctgc
   103021 actagctgca attaaggtat tgtttgatgt atttatatat acatataatg cagataacaa
   103081 ctgtccggca gttttatccg caagtattac attaatattt tcaagcaagg ctgaagggtt
   103141 ggagcataat ttactggaag agtgaaatgc tattttgcac atagatgcaa tgagagctgc
   103201 ggaaacaccg tggccggtga tgtcagcaat aaagattcct atttcatgtt ggcttataat
   103261 gatatagtca aaaaaatcac caccaactat ttcggcaggg atatataagg atgatatcct
   103321 taaattttga tggagtgggg gaattgcagg aatgattgat tgctggatac gacgtgctgt
   103381 gttcatgtct gctttactta gagcgattga agtttgaagc aatgtgtact ggcggataag
   103441 aataaatgaa agagataata aaagaaaaaa taatccccaa tgaatatatg agcgaaaatc
   103501 ataagttaaa tagccaatag caacaacaac actgtgcaat actgttatac ataacacaat
   103561 cagtccggca agtaaaatgc cagcttcctt attacctgct ttaaattgat ttatcataac
   103621 tgaaattata ataattacat ctactattag cagatattga aatatgtaac gcacacccaa
   103681 aatatattta cttgcatgca tattccccgt aaaatataat tcaccaatgc cagcaagaag
   103741 aacaattgtt tcaaaaatgg cataatatgt gttaaataca aataatattc ccagtattga
   103801 ttttttttga taattaaaga ttacttttgt aaatccaata aacatagtgg tagaccaaac
   103861 ggatgaaagc agccacagac atatccataa caagggggta tcaagtaaca aatctcgtat
   103921 tgatgtataa ttaagaatat atagaatcag taataaagca aaaaaaccaa aataaaaata
   103981 ttgccactgt ttcctgttaa taataaatat tgttaatgaa aaaaaaccta tgaaaaaagc
   104041 tatactggca atgattatgt tggcaatatc agataaaatg atgttcttaa tcagtgtata
   104101 ctcagtaccc agcaatacag gtcctgttat tccgataagg tggtaatttg attggatttt
   104161 aaaataaata ttttgacctg ccgcggaatc gggcaaagct ataaagtgag atttttttgg
   104221 tataaaatcc ttgctaaaat ttgcatctgt atcccagaat gagtagattt gtcttgaatc
   104281 aacgtacaca accagtgcca gatataaatc tcggataaaa agagctggat gcattggcag
   104341 tttatcgggt aatgttgtac ggaaccaaac agtattggat tcttttttca attttgaaaa
   104401 gggatatatc actttatgcc agttactatc gccagtatta atattttcta aggaaaaatt
   104461 ccagctatat tcccagttat cggagagcag ttgaggatct tcatataatc ctgattggcc
   104521 agatgtaatg agatcacaac ctgttaatat gatgagcagt aaaggaaggg ctataattat
   104581 atagatcagt tttaataagc gtaaaccaga aattataatc atttatcgcc aattattcat
   104641 aaacagaata ataaaaaaat attacttata accccagttc aattgagaag tataataagc
   104701 aattaatggg gtaaaaaagt agttttaaaa tcaggtgcat tatcctgagg tcgatttagc
   104761 ataaaataac cacaaggata ggtccttatt tgactaaaat atagaatagc agtaaaaaag
   104821 tccacccaca aatttattta gaagaacgga aagaaattgc ctgtgtgctg gaagaaggat
   104881 acacaaataa acaaatagcc attgtactgt gctgatcttc ctcatccata tcatgggaga
   104941 tgtgatgtac tcatagtcaa gacttcatac cacatatcgg acgcaccatg tcaatatacg
   105001 ttcagatgga agaatgtgat tatctaacac aaaatacatt tgaagagtga tagtataaaa
   105061 caatatgtaa taaaccggtt attggacggt tgtaatcctg aacagattca agaaggatgc
   105121 gtattgatca tacggatttc aaggcaaatc atgaatcgat ttatcaatat atatcataga
   105181 catttaataa aacaacgaaa agagttggta aaaaatgcct cagggagtta tgattagatg
   105241 acaaccggac acttaactat tgcacttcat tattggataa agcttttctt cattaatgtt
   105301 attaaaagtt ttgttaactt gtaaacaact attacaactt attatgataa taaggttgaa
   105361 aataccttca atgtaaatac attttatatt gttttctctt taccctttgg ataatctata
   105421 tacaacctaa caatacctca atctgaaaac agataattgc taagttcata ttgataataa
   105481 ccaattttga gatataactc acctagaaca aataatcttt acaggtaatg agcgtttctg
   105541 gtatatatgt cttattacat tgtaatagct ctcctggctc aatggataga gcgtcggcct
   105601 ccggagccgt gtgtctgggt tcgattcccg gggagagcgc atacttgctt tctatgtata
   105661 tgattgcata aacaaaaata gcaaacagtg gcacaaaaaa attttattac ataagtcagt
   105721 tttagttgtg gcgatagtta ctattatagc cgggatcact gccgcaaatg ctcatgcgtt
   105781 gcgcacggtg ggttttatta ccatgcttgt ggtaatactg catagtgtga caggattagc
   105841 gctggggtat gctgctgctt cgttgcttgg attggataaa agtgtgcgaa aaaccattgt
   105901 ttttatcaat accaagcaat gaagataatt cattatacga tacttcattg ccaatttgta
   105961 aagctaatgc ctgtagtagc ttttcaagga tttcaggata ttttatctgc tgatagtgca
   106021 gcacatcttt atatgattat ctgaaagcaa gattttttaa aagtataata gtattttcct
   106081 tattaagtac tatctcagga tacatgccat atatcatgaa ttgcggtaat aggcggtcaa
   106141 tttctaaggg ggagtaaata gattgcaatt ttcgtattga aaacgggaat aatcggaatt
   106201 caatttttct tcctgttaag gcttgcatga tagtgcttgc taatttaaaa gaagatgaac
   106261 catcagcaat aattaaaatt ttccaaaaaa atcccctaag ttccattaag aagtgtaatt
   106321 atataataat ttatataaaa attattatta taataacgat aagacaaaat tttgaaaaaa
   106381 ttataaataa gttgtcaaaa ttttactata ctattcatat agtagcaaaa atctaaaaag
   106441 agtaacaggt atgtttgcta tatttcaaaa aggtggcccg gtaatgtggc ccattttgct
   106501 atgttctgtt ctgtcgctgg caatatttat agaacggtta tatgtattaa taaagtcccg
   106561 accatatgta tgggattcaa ttaatgtttt taaacagcag tttattacaa atgcattgaa
   106621 taaaaaaact attcccgaat gccccgggat agttaaggat atggctgatg tcctggatct
   106681 ggaaaaagat tataccatag actatgaact gcatgaagcc gagcataagc tggtacgata
   106741 tgccacgttt tatgtgcgta acttagaaca acggttaaac tggcttgcta ttattggcaa
   106801 tattgcacca cttttaggcc ttttggggac tgttaccggg atgattaagg tgttcatggg
   106861 tattcagaat atgcaggggc aggtcaatcc gtcagcgctg gcaggtggta tatgggaagc
   106921 gctgataacc acagcagcgg gattgattgt tgccatcccc accattattg cttatcatta
   106981 tttcgaggac aggattgatg atattaccgg ggcgctgaaa gagttaatta gcgatatatt
   107041 agaggtaata aagcaatgag tgctgaaagg ctttttattg aacgaagaaa gaaagtagtg
   107101 attgatttga atatagcacc gcttattgat gttgtgttcc agctattgct tttttttgca
   107161 ctcacatcat attatgttgc caatcctggt attgaagtat cattacccaa agcaaattcg
   107221 gctgttactg tgcaaaggaa caatattgaa atatatatta ccggtagtaa tgccatttat
   107281 tgcaatgcca caaaggttga actatcggca cttaaagcaa tgctttccac actgtcagct
   107341 gcttcaaaga ctgttatcct gaaggctgat aaaacggtac aacttggcat ggtggtgcag
   107401 gtgattgata tcataaaaca aacacaggtt aaagacctgg tgatagcaac aagcatggaa
   107461 gaaaacaatg cctcacaaaa atagaaacat tgtttattat gcattcttca tttcgattgc
   107521 tgtgcatggt gttttcctgt gtgtgtcatt tggaggcagg acaacggata ttcaaaaaca
   107581 tacattgaat gttaccatgc acacatatac aatattgcca catgttgaac atgctgctga
   107641 tacaaccagg ctcaagaata atcctgcaaa gcctgttaaa gagggtggtg tcatggcatc
   107701 gcaatacatg gtttccaatg aggacagcca gacagtaacc atgcaatata ccgatatgat
   107761 acgacagcgt attcaggaat cgttagtata tccggctaca gcacgtaagg acggtattga
   107821 aggaaaaacc tatattaaat tcaccatcga taaaaatggc aatctactgt cggctatcat
   107881 ggtacgttct tcagggtcag cacttttaga tgatgctgcg cttaaggcaa tacacagcgc
   107941 ttcacccttc ccagctattc ctgatactat tggcagagag cgcatgacgt ttgtgcaggg
   108001 gttgacgttc acgttgaagt agtctacttc gtcaaaagcc caatcacttc ggtgtgatgg
   108061 gtgcatggga acatatcaat catatataat ttttgtaagc gatagttact gtcaacaaag
   108121 cttttgatat cacgggcaaa ggttacagga ttgcaggaaa catatataat gcacgaagga
   108181 ttcatggcaa caatggtctt tcttcctcgc tttgatatgc cctgacgagg tgggtcaata
   108241 ataatacatt catgagtatg tttaaaaggg tgatagtttt caatacttac cgtatgaaaa
   108301 tcaacagtaa cattattacg ttttgcatta taccttgccc attcaatact ctgaatatta
   108361 gtatcaatgc cagtgccttt cattcttttg gccaaataaa tactaaaaaa gccaacacca
   108421 caccccacat caagaattga tgcaaagctt tgcgatagtt catcaacaac aagaagcatc
   108481 ttgcttctca ggaatttgtt tgcctgaaaa aatgtactga cacttcgttc ataggtgatg
   108541 ccacattcct gttcagtgat aactgcagtg gggtcagagc atattgagct ctgtgcatcg
   108601 atagcaattt tgataggttc gttagcgtgg ctatcacacg attggctgag gataaatgta
   108661 tttagctttt catgaagaag taagcaaccc tttgcaggta ttggttcaac actatgcgaa
   108721 tcttttttat aaaagcctat atgagtacca tctgattgaa gtgtggcgtg gcttcggtaa
   108781 tggaatcgtt cacctgaaat taattcaatt gctggcagga tatgatgaga aagaccacca
   108841 atgtgtgtta acgtattcat gaatagctct ttttttactg taagttcctc tttgtaggga
   108901 atatgaagga agtcacatcc tccacagtga gtaaatgcag gacattgtgg ggtaatacga
   108961 ttgggcgaag gggttataat atcggtaatt gaagcaaaac agtagttttt tgcctgacga
   109021 gttatggtaa cggcaacagt atcacctacg gcactgtagg gaacaaatac ggttttgcca
   109081 tcaataaaac caattccata gccattgttg gcatatcgct gtatggtgag agtatgtgtc
   109141 atagtatttt taccactttt atcccacgtt ttccgcacac tcggtgctcc ctgcgatcgt
   109201 gtcgtgatac attaattctt taatctcacg tagagatcgc tgaggacaca gaaatgatac
   109261 catatatggc tctgactcca ttgtatcatg caaatgaagt catttcaggg ttttatttgt
   109321 tatgaaatta ttgcaatgcc atatttcttt ttagctctgg tgctcattac gcaggcgctc
   109381 aagcgcttga gcgcctgcga tggttgattt taaatccaaa aaaaatattg acaatttctc
   109441 caccatgtga aatactacca aaaaggtcgt aatagtagca aaaaaggaag tacacaatga
   109501 gattatcaac cagaagcaga tatggcgtca ggatgatgtt tgaatttgca cttaaattca
   109561 atcagggtcc tgtttttttg aaagaagtgg caaagatgca ggggatatcg tttaaatatc
   109621 tcagcaagct ggtaatcccc ttaaaatcgg ctggcttgct ccaatcggtg cggggtgcca
   109681 atggtggata tatgctggca aaacatccat cagaaataac catacgcatg attgtggaaa
   109741 aattggaagg cgatgtatcc ctggttgaat gcactgaaaa tcccaaagtg tgcaaacgca
   109801 atgtcgattg tgtggcacgc acaatctgga aaaaggctga tgacgcaata atgcaggtgc
   109861 ttgatggcat cacattacag gatatggtag atgaatataa gcacgctgct ggaagtatat
   109921 acgaaatttg aaatgataaa aaggagatac aataatgcaa tacactacta tcatagaatc
   109981 cattggcaat acaccactgg taaagattaa taaacttgca cctggtgggg ttaatcttta
   110041 tgcaaaggta gagtcgcgca atcctttgtc aagtgtcaag gatcgtattg gctatgccat
   110101 gattgcggat gctgaaagca agggcttgct tcatcctgat tcggtgattg ttgaacccac
   110161 cagtggtaac acgggcattg cgcttgcttt tgtagcggct gtcaaaggct acaggcttat
   110221 attgacaatg cctgatacca tgagcataga gcgccgtaag ttgcttgcaa tgcttggtgc
   110281 tgaaattgta ctcactgatg gtgcacttgg catgcgtggt gctgttgaaa aagctgagga
   110341 gatagttgca aaaaccaaca atgccttcat gccgcaacag tttaacaatc ccgcaaaccc
   110401 tgagatacat cgcaaaacaa ctgccgttga aatatggaat gatacaaacg gtgatgttga
   110461 cgtctttgtt gctggtgttg gtacaggagg cactatcacc ggcgttggtg aggtactcaa
   110521 agcaaaaaaa ccatcggtaa aaatagttgc tgtggaacca gctaaatctc cggtattgtc
   110581 gggaggaaaa cctggtcctc ataaaattca gggcattgga gctggtttta taccggcagt
   110641 cttaaaccaa tcaattattg atgagatagt aaccgttgat gagactgatg ccggaactat
   110701 cgccaaaaaa ttagcaaaag atgaaggtat ccttgcgggc atttccgctg gtgctgctat
   110761 gtgggcagcg ctgacaattg caagtcgtcc tgatagcaaa ggaaaaacaa ttgtcgtttt
   110821 attgccagat tccggcgaac gttatgtgag cacctggcta tttgattaaa gcaagaaaag
   110881 ataatgatat atcatctaca atggatcatt taataaaaag gaggaacaaa atgtcaggta
   110941 actatcgcat agaaactatt gcccttcatg ggggtcagag catcgatgcc acaaaagctc
   111001 gtgcggttcc ggtgtatcgc accagttcgt atgtgtttga ttctgcacag cacggtgcag
   111061 acctctttgc actgaaacaa aaaggctaca tctatacccg catcatgaat ccaacgcagg
   111121 atgtgctgga agaacgtgtg gccatgcttg aagggggcag ggcagccctt gcactggcat
   111181 caggaacatc ggcaatattt tatgccatta ttaatgtgtg cagggcaggc gatgaattta
   111241 tcagcgcaag caatctgtat ggtggtacct acaccatgtt tgatgctata ctgccgcagt
   111301 ttggcattac ctgtaaaatg gtgccctaca atgattacga tgcctacgaa aaagcaatat
   111361 caccaaaaac gcgtgccatc tttattgagt caattggcaa cccaacgttg ggcgtgcctg
   111421 attttgaaaa agttgcaaca atagcacaaa agtatcatgt gcctcttata gtggataata
   111481 cgtttgcaac gccgtgtcac ttcaggccca ttgagtatgg ggctaatgta gtggtgcatt
   111541 cgcttaccaa gtggttagga gggcatggca ccgccattgg tgggatagtg gttgatgggg
   111601 ctaactttga ctggactgac acaaagtttg cactctacaa cgaacctgat gcaggttacc
   111661 atggcatgcg ttttgcccat gatctgggtg atatgaacag ccttgcgttt atactcagga
   111721 tgaggcttgt tgcgctgcgc aatctgggtg cagcaatctc gccggacaat gcatggatat
   111781 ttttacaggg gcttgaaaca tttccactca ggatgcaacg gcacagtgaa aatgcgctca
   111841 aagttgcaca atttttgcaa aaccataaaa gtgtagcatg ggtgcactat ccgggtttag
   111901 tgggcgatag ttcctacaag gtagcttccc gctattttaa caatggtttt ggtgggatgg
   111961 tagtctttgg cattaaaggt ggatatgaaa gcgctgtgaa atttataaat tcaattaagc
   112021 tattttccca tttagcaaat gtgggcgatg caaaaagtct ggtgctgcat cctgccagca
   112081 cttcgcattc acagctgtca gaagagcagc agcgagaagc aggcattaca ccagatctta
   112141 tacgcctgtc ggtggggata gaacacagtg atgacatcat tgcggcacta caggaagctt
   112201 tagaataaaa ggtttatcag taatccccat aaaatcatga tttatactac atactatgta
   112261 taatacatga aatcatgata gctacatgct gactgagtgt gtggcaattc tctcagtatg
   112321 ctatcattgt gattcctctt atggtatata ataaaaatct tccagaaatt attaccattg
   112381 cgtatataca tgtgttactt agtgatttga ttttgatttg acatagttgg ttttaaatga
   112441 taacctatta tagtaaatta tattttctgc atagatagtg aacgtaatga ttgctatagg
   112501 atatataatt cagctacaaa tcaatacagt tcacttcaga aagatattat aataattgga
   112561 ggaaaggtat ggttaaaaaa attctgatta tactgctgtg cctcattgtt gtgcttggtg
   112621 cagtgtatgg gtatgtgtat tataaggcat cgaaggttcc tgaaaataat gcacaggcta
   112681 tgttgaaagc tgcactgccg cacgataaaa agattgtggt atgttttggc gacagcttaa
   112741 cacatgcaac agtgagtttt gattatgttg gtttcctggc taaagatcct gatttgcaaa
   112801 cctatatatt tgtaaacgag gggattaatt cacggttggt gtataatctt cttcaggtgg
   112861 ttgacactgt tgttgcactg aaaccacact atgtatttgt atggattgga accaatgacc
   112921 ttaaaggctc gcttaatgat aaagaatacc agcgttacaa tgatctgtgg aatttgccac
   112981 aaaagccaac aaaagaatgg tttgagcaaa actatcgtca attagtcagt atattgaaaa
   113041 caaagacaga tgcaaagatt gtgctgatat cgctgccacc cctgggcgaa aatattgaca
   113101 gccttccatt taaacttagt atggagtact ctgccattat cagggatatt gcacaaaaag
   113161 aaaaattggg atatataggc ttaaatgaga ttcttacccg cgatttgata cgtgaaggca
   113221 aacgcgatgt tgccccatat actactggat tgtggtttat gtacagcgca attatacagc
   113281 attatctttt tggcagggat tgggatacaa tttctgatag ccgcggtctt acctatatga
   113341 ccgataacat ccatataaac ggcaaggctg gaagaatttt agctgctgcc attaaggaaa
   113401 aacttattag caataaataa taaagccttt aatcaggaag gcattgttat atacagcctt
   113461 cctgattatc agtaactatc gccctaaaag gtatggctga aatcaacaga tacggaagag
   113521 tcttctttac tcattccata gtagaaatgt acaatcgtgg ccatattcca tgcaataacc
   113581 agccctccac cataaccctg atggtaatcg ctgaaacgtg gatcagtaaa tggatctgct
   113641 gctttgtcgt aaacattgcc cacatcataa aatacggtta tcttaaactg gaaattctgc
   113701 ccccagggat ttgcttcagc aaatttccag cgcagttctg cgtttgcaac agtcatggtt
   113761 ggaccaacaa aacgctgctc gcgataacca cgcagagtcc gattgttacc aagacctgtg
   113821 cgacggttga gtgaaaatcc aaaataaccc atttcaaaga atggggtgtc accattagca
   113881 tcagtgtagc ctgcgcgcaa tgccagcgtt aatgattgca gtagtggaaa ataatattga
   113941 agctggacag tgtggcgtgt gaagtcataa tccgaaccta tggtatcgtc agatatttca
   114001 aacgcatagt caatgaggta tcctgttttg gggtcaggtt cataatcacg ggtatcatag
   114061 tagatgcctg cacggataaa gtttgaccat ccgccatcat accctaatgg ctgcagttgt
   114121 tgtaggagtg ttggcccctg aggggtgccg tcaaactttt ccccatccca ggtgtcaatg
   114181 gtcactttct ttacttcaag gccagcaaga aatttaaggt tctgagttat atccctgtaa
   114241 atattcagaa aatagcttgg cttggtgtat tcataattgt agtactttaa gaaacgttca
   114301 tcgttatcag cataatctaa ataatcctgg aatttatcaa atgtttcata tgctgttgga
   114361 tacgtagtgt aaagcgttaa ttttcctttt gcagtatcag caccctgacc aaaataatta
   114421 gcattaatct ttttatcata ggctattgaa gtaatgattc tgaatttagt gccaaagata
   114481 tagggcatgt ccaaatttat ttcgtggtac tgatatccat tggttgtttg ataaaactgt
   114541 ccgtacaact gcatcttata cggtgcatag gcaaaatatt cgtcatcgcg tgtgccatta
   114601 ttatacagat aaacacgtac accgtatcca aacccattat cgctatcata attaaccagt
   114661 ggtaatcctg ttggatacca gccttcaatt ttttttgcca ggtcttcatc agagagacgg
   114721 gcagccattg cattttgtaa tgcaaaaaac aaacacaaga taataatgca tactacagtt
   114781 tttttcatag tgcctccagt aataaaataa tataacggcc tggtataaat cttacattta
   114841 aaaattgata ttattgtttc gtgtatgcta attgttcaag tacattcaga tcaagcttgc
   114901 ccatcatgat aggactttct tttatcattc ccagtatcca tggataatca tggtgtatat
   114961 cactggtata ataactaaaa tacagtgtgt tgccttgtat cacatatcct gcataactgg
   115021 tatcgcctgc actgggcaca tctgaaagcc atacaagttt ttcaggtgtt accagccaca
   115081 gggatgtgcg cttttttgcc agtatgctgc cgtatttatt gagtaaccct ggtgtatagg
   115141 gatttcttct gccaattgca tatatcttac cattgaatgc aaagagtgct gggccatcca
   115201 ggcgtgtact gtaatccttg atgcctgccc actgtgtgta gggaggtttt gcaatgcgta
   115261 taccagtgca tgcacggcta tcgcctatcc acgagtctga atattccagc cgctgggtgc
   115321 ttatcattgt ggcatcaggc aaaaattcaa tagcggtttc gtcacagcga tcaccttcat
   115381 aaattaaaga gacaggttcc cagctggtgc catcagttga tgtaaagagc attgcttttc
   115441 catgctcatg ccagtaggct ggaacatacc accgcttccc atcaaaagtc tttggcctcc
   115501 agaagagcca ccctttatga gctatctcct gcagggaact ccatttcttg ccgtcactgg
   115561 aaattaaata cgccgttgaa tagggttccg cagtaaattc aatgcttttc aggacaaaca
   115621 gtattaattt gttaccaatg gctaaaaact tgggatctcg aatgtcctca cctggtattt
   115681 gaatcgtgga aagcttttcc cactgcttac aatctgtaga acgccatact ataagcttgc
   115741 actctggagt tgcaaaatgg tacggcgatg aagcatggac aagataaaag taattattaa
   115801 aatataccat atccgtattt gagttgtgca ttccatcacg tactgcttcc catacttcaa
   115861 ggataagaga tggatcaacc tgtgaagtgt ttggtcgtat aaaccacaac gcaataataa
   115921 gggtcagagc tataataata ccaaaaataa aaaaaccaat aatcttccat tttgtcatga
   115981 cgatagctcc ttaaacaaga gttatgtgca tgtaccaaac aatataattt tgatatacat
   116041 tcttgttact agtttttaat ggtaccatgg tatcatatag agtgagtact tgctctatca
   116101 cactaatatt atattgtcaa tgttattttt ctttacaagt aacctgcaat ttattactat
   116161 tacatcattt gaacaattag ttgttaaaat tatagtataa tttatacaat aaaattttat
   116221 aagagaggtc tttatgaagg atgtggttat agcagcaata gttcgtactc ccgttggtac
   116281 atttgggggt gctctgaaag atgtgaccgc tgtagaattg ggggttgtgg ccgttaagga
   116341 agccataaaa cgagcaggta ttactcccgg gcaggtagaa aatgttattt tagggaatgt
   116401 gttacaggct gcacagggta tgaatcctgc acgacaggtt ctcatttatt caggtattcc
   116461 acaggaagta cctgccatga ccattaataa agtgtgtggt tccggattgc gtgctattag
   116521 tttggctgct caaattatca aggcagggga tgctgatatt attgtggctg gcggcattga
   116581 gagcatgagc aatgctccat ttgctcttca gaaggcgcgc tggggatacc gcatgaatga
   116641 tggtgtcttg atagatatca tgataaaaga tggtttgtgg gatgtgttca atcaatacca
   116701 tatggggata acagctgaaa atgttgcagc aaagtggggt ttaacccgtg aagaattgga
   116761 tgagtttgca ctggggagtc agcaaaaagc tgaagcagca atcaagtcag ggcgttttaa
   116821 agatgagata gttcctgtga caataaaaga caaaacaggt gaaaagcttt ttgatactga
   116881 tgaacatccc cgatttggta caactatgga agcactgtca aaactaaaac cagcgtttaa
   116941 gaaggatggt gttgtgacag caggtaatgc atcgggtatt aatgatggtg ctgcggcagc
   117001 agttgtcatg tcagcacaaa aggcaaagga acttggcatt actccattgt gcagaattgc
   117061 atcgtatgcg tcagctgggg ttgagccagc cattatggga acaggcccca tacctgcaag
   117121 caagaaagca ttgcaaaaag caggatggaa tgtgcaggat ttagatttaa tagaagcaaa
   117181 tgaagcattt gctgcccagg ctgtggtggt taacaaagaa atgggctggg atgttacaaa
   117241 ggttaatgtt aatggtggag caattgcgat agggcatccc attggggcaa gtggtgctcg
   117301 tattttaaca acactaattt atgaaatgat taagcgtaat gctaaaaaag gcatagccac
   117361 actgtgtatt ggtggtggac agggtgttgc agtaacagtg gagcgatagt aactgttgaa
   117421 aattataatt ttaaaagggg agcctgggtg ctcccttttt agttgaaatc aaaactgtgt
   117481 ataaaatgaa tataatttga tttgcttgaa aagttaatca gtagtattac tggatacagt
   117541 ttgaatgaat ttaatattaa ctgtaccaaa atcgggatgt aatgatttta tattttgcac
   117601 ctgtttgagc gattcttcat cagcctgttg tgacagcact ataactgtta catcaagttt
   117661 tccctgattt tcatcaaatt gaatagcaaa aacatcggta ccaaaatatt tttttgctgt
   117721 tacccatacc ttcttttttg catgttccag tagaaaggtt ctgtaagtat aaattcctac
   117781 cggtattgcg ataatggtta acagtataac tgaaataata atctgtgaaa gagctctccg
   117841 ggtaacgttt tccttttcct ctctggattt tggatgtatt ttcataatcc agaaaataat
   117901 tgcacctgcc agtgaaatgg atatcaaatt gattgaaaaa agaataagtg ctccacgtgc
   117961 aatatgccag tcaaagttgc caactccaat gccaatggtg cataatggtg gcattaaggc
   118021 cacagcaata gctataccta caagcgtatt gctgattttc ttatttgcat tgccatatgc
   118081 accaacaagg cctgatgaaa tggcaataac aatatcaaaa agatttggtt ttgttcgtgc
   118141 aagtatttcg ctggtatatt cagcaatggg gacaaaaaat gccaaagtgc ctgatatcaa
   118201 aattgccaga actgaacctt taataattga actaaacgat gttttaataa gctgtgtatc
   118261 accccagatg acccctaatg aaaacgctaa tattggtgtc ataagtggag caacgatcat
   118321 ggcaccaata attactgctg gcgaattctg cagaaggcct gcggttgcaa tgaggcagga
   118381 taacaatgtc aataaatagt attcaatagt tgggcgtgca ccgtatgaaa gtgtttcata
   118441 tatatcaagg tcctcttttt ctttttcacg aattttttta tattcattgt attttgtccg
   118501 ccagaaatta atgttaacat aattacgcat ggaatgttct gcaggttgaa taactgtatc
   118561 aaccatattt ttaaatttct ggacaagtga atcttgaact ttttcaggat ttgtttcatt
   118621 attgttttgc tttttcataa tgcactcctg aagctggaat gagatttacc aaatagtatg
   118681 taacacataa tactgaaaac aattgattaa atcaagtaat aaataggtaa ggttgctgtg
   118741 catttttatc atttcagatg ttattgcagg ttttggtgca aaaatatata atgcttcaga
   118801 taacaatact tatctatgga ttttaccacg tgcaagtgat gcaaatatcc cgccaaagca
   118861 cagcaatgtg aatattataa atgctgtttg tgaactttgc aagaacctgt caaaatacag
   118921 tggggttatc tgtactcttc ccatatgtac tgccagaatc aatgtaacaa tgcccatact
   118981 gaaagcctgc ccggttaggc gcattgtccc cagtatacca gaagcaacac cgtaatattt
   119041 tttatgtacc gagctcatga cagcattggt attgggtgaa gaaaaaagtg ctatgccaaa
   119101 acctatgaaa attaagcagg tgatgatgaa taaaattgat gtatgtaaat gtaaaaatgt
   119161 taagagcaga agcccaattg tggtaaaagc cataccccag gaagcaataa cctgaggctg
   119221 aattttgtcg gacattctcc ctgctatagg tgaaaacaga gccatggtaa caggctgtga
   119281 aataaggatg atgccagcac tttgcgggct tattcctttt atatattgca gataaaggct
   119341 tacaatgaat gttactgcaa atgttgcact gtaattaatt aacgctgcca gatttgaaaa
   119401 tgtaaagaca gtattattct taaaaagatt catgtcaaga acaggatgtc tggtgtgtag
   119461 ttcccatttt ataaaaagga aaatgaaggc aattccacct attatcataa ttgttccaaa
   119521 tactgacgga agagatgaaa atccaaacat caccatggtt aacgcaattg catagatgag
   119581 cgatccagta aaatcaaacg tttcaccgtc agcttcaacc cattctcctt ccagtttgga
   119641 atatattaat agaactatcg ctacccctac tataacactt attgcaaata tactgcgcca
   119701 tcctaaatgt tgtgttaaaa tgccccctaa aaaagggcct gttgaaagcc ctatgtaaac
   119761 cgctgaaaca ttaataccca gaacacggcc ccgctgctgt ggagggaata ctgatatgag
   119821 catggcagcc cctgtgctga atatcattgc accgcttatt ccctggagca ctctaaatag
   119881 aatgagtata agggcagatt gtgcaatagc tgtgaggaat gatgaaaaag tatagagaat
   119941 gacgccgtag gtaaatattt tcttcctgcc gtagatatcg gctattcgcc caaaaggtac
   120001 cagaaacata gctgcagcta atagatacga tgttgcaatc cagcttaaca caattgcatc
   120061 aatggcaaat tccttcccta taataggaag agcaacatta attgatgaac ccataaatga
   120121 tgtgagaaat gagcctaagg tggtaaccgc taatgctgtt tttctggttg caggactgac
   120181 tttatccatg gtagatatcc ttatgtacaa tgagaaattt atgccattaa ttttaaagta
   120241 tagtatcaag acatataaaa atatatttag gtactattct accagcttat cataatcttt
   120301 ctatataaaa aatatagtcg aaaaacctat taacactatt tatcagtaag ggggaatgta
   120361 ttgtgtagtt gaattaataa taaagcaagt aatagtaaca ggaggaaagg attatataaa
   120421 aaatatgtat tgatagagaa taaaaattaa ttattttagt gaaagtgccc atttattagg
   120481 acatttgcct attgactaaa acaggcgatg atgtataata aagcacaata tatagaaaaa
   120541 atggattaaa tatatggcta ttgtaccaga gcttataaag gtttttatta tagaagatga
   120601 aaaccttgta cgggataccc tggctcagag cctggagcta gttgataata ttaatgttgt
   120661 tggcacttct gaaaacatat cctcttctat tggagatatc aaagcaacaa aaccagatgt
   120721 gcttattgtt gatgtgcgcc ttaaaaatga aaatgggatt gaagccagta aaaatgtttt
   120781 aaaattaatg cctgctgtaa aaataatcat actttcgggt tattttaatg actcgcttgc
   120841 tgcaaatgca ctggcagcag gagcttcggc ttttttatca aaatccattg caatatcaca
   120901 tctggtggct gctatatttc atgttatggt tcataatagt ttttatgcac cacagttacc
   120961 tgatgaagtc cttgaagata taatggtgaa gaacaaaaca tcaaaaagac tatgtgcact
   121021 ttcaaagcgg gaaaaagaag ttttgcagct tattgcaaaa gagtattcaa caaaagacat
   121081 tgctaccatg cttggaatca gcgaaaaaac tgtccgtaac cataaatcca acatcatggc
   121141 aaaattgggc attacaagcc acgttggcct tatcaagtat gcatactaca tggcaatgat
   121201 ttaataatag cttataaaaa acgttaggtt ttttatcagt tactatcgcc caatgtacta
   121261 ttttttacgt ttaagaaact tttcaatttc aacggcaata aatatcacag atgataatgc
   121321 aagtgttatg ctcaattcct caagtgttaa tggatgcgtt ttaaaaatcc cattgaggta
   121381 gggaacgtat attgttgcca tttgtagcac aaatgtgagt ataaacgctc cgagcagcgg
   121441 tttgtttgag aaaaggccaa gcgtgaacaa agactgtttt tccgaacgta tggcaagtac
   121501 atgtcccatc tggcttaaac acagcactgt gaataccatt gtttgccagt gggcattcca
   121561 taatgttatt gaaagtgctt gcgtaataat agaaacagct cccatgagca atcccaccca
   121621 tataatgtga gcacccaggc catgagcaaa aatgctttcc tgcggatgcc gaggtgggcg
   121681 ttgcatgatt cccttttctt cgggctcaga agctaatgcc agcccaggca aaccatcagt
   121741 gacaagattt atccatagaa tgtgtatagg aagcagtgga atgggtaagc caaaaaatgg
   121801 agctaaaaat attgtccata tttcacctga attgcttgtc atagtgtatt ttataaattt
   121861 tctaatattg tcaaagatcc ttcttccttc tctgactgct tttacaatgg ttgcaaaatt
   121921 atcatctagt aaaatcatgt gtgctgcctg ctttgataca tcggttcctg ttattcccat
   121981 ggcaacacca atatctgctc gcttcaaagc aggtgcatca ttgacaccat cgcctgtcat
   122041 ggcaacaaat tggcctttat cctgcaatgc ttttacaatc tttagttttt gttcaggggc
   122101 aacccttgca taaacgctaa tgtattctac cttttgttca aattcttcaa gcgatagctc
   122161 ctgcaattct ttgcctgtta taatataggg tcggttttca tcaataatgc caaggcgctt
   122221 ggctatggct ttagctgtca gtggatggtc gcctgttatc atgactggtt tgatgcctgc
   122281 tgatttacat tcagacacag cttcttgtac ttcctggcgg ggagggtcaa tcatgccaac
   122341 aagacctaag aatgtaagat ttgattcaac tgtttcaggg gttattgaag aaggaagagt
   122401 atcaagaaat tttgatgtaa ctgccagcac tcgtagtccc tgctcagaga gttgttcagc
   122461 cacttttaaa atttcatctt tgttgatggg tactatctct gaatgaatca tgatagtgtt
   122521 ggcactatct atgatggtat caacagctcc tttggtaaat acaatgtacg gtgattgggt
   122581 tatttcctgg tttgtatttt tatgaatggt ggtcatgcac tttctttcag aatcaaacgg
   122641 tatttcagct acacggggaa atgttcgagt gagcaaatct ttatcaaatg aattggtaaa
   122701 ggcatagtaa tacaatgcag tctcggttgg atcgcctaac agtgtgccag atgtatcacg
   122761 tatggcatca ttattcagag ccagagcata gaaaaataac tgtgaacttt tgctacgatt
   122821 gtcttgtagt ggttgagttg cagtaaacat tgtgttattg acaaaaattg tttcaacagt
   122881 catcttattt tgtgtaagcg tacccgtctt gtctgaacaa atataggtga ccgaacccaa
   122941 tgtttctaca gcaggaagtt tgcgtataag ggcgttttga ttcaccatct ttcgagcacc
   123001 cagagccagt gaaatggtga ttactgcagg cagtgcttcg ggtattgctg caacggcaag
   123061 tgaaattgcg gtcaaaagca tgcgcagtgg ttcttcgcca cgtaaaaggc caatgccaaa
   123121 gataactgcg cagagaacaa gaactgctat agaaagcttt ttgccaaata cagctaaacg
   123181 cttttgcaat ggtgttttta cctcttcttc ttcctggagc attgttgcaa tttttccaag
   123241 ttcagtattc atgcctgttg caacagcaat tccacttcct cttccatacg tcacaattgt
   123301 gccgttatat gccatgtttt ttcggtcacc cagagggagg ttttcttcat tgatttgctg
   123361 tgtttgtttt tccacaggaa ctgattcccc tgttaatgct gcttcttcaa ttttcaactg
   123421 ggcagcttca ataaggcgca aatcggcagg gattattttc cctgcttcaa gcaatactat
   123481 atccccaggg actatttcac gggcaggtat gttcacaggt gtgccttcac gtatgacggt
   123541 tgccagaggt gcggccattt tttttaatgc ttccatggcc ttttctgcac ggtattcctg
   123601 gacaaagcca atgattgcgt tgagtactac aataactata attgctatgg tgtcagaaag
   123661 ctcacctaat aatcctgaaa gcaatgctgc tgcaattaat accagtatca taaaatctgt
   123721 aaattggtgc aggagcatca taagcggtgt cattttcttt ttttctttca gttcattaaa
   123781 gccatatgca ttaaggcgta cctgtgcttc ttcctgcgta attcctttat atgatgtttt
   123841 taagaaagta aaagcatctt caacagacat tgtatgccag ttcatgcatg ttccctttct
   123901 ataatagtaa tagtttaaat atgaaacggt actatattta ttatgttgat attgtacaaa
   123961 caatgttaca ttacaatgta tggcaaaaaa tatcaaatag aaaaagtaat ggaaaggtat
   124021 ttggtatata tcattcagac ggtatccgta gtaattgcac tgctatgggg tacgtgtcca
   124081 caatacagta ggctttcact tttcctttta cctgaccttt gtcaaattca atggagattt
   124141 cctgtacagt attgtttata tccttaactt tagtgcgctt ggtataatac agcggtagtg
   124201 gattccccgt ttcagcatca acaaccatga tactgtaaac atgctcatcc ttctttaagc
   124261 gggagtcttt caatagtgct tttatgaagg ttttccctgc tttaaagctt atcgtgccag
   124321 caccctgtgg tgttgtggca acaccgctac catgcaagct catagttagg ccgccaaaca
   124381 ccgccatatg ccctgtatca aattcggtca ttcccattag tttcaagaat ctgccataaa
   124441 attcaatctc atcgccaagt gcaatggcaa ctagtgcggg ggagtgttcg atagttcctg
   124501 ttaaaggatt agctgttgtg gaaagacggt acatgccaaa gggcatatcc cacgaaccaa
   124561 taaaattaat cttgaaccca tcatagttat agagagtaac acattttcca tcactccgca
   124621 aatataatgg atagcgtgcc tcaagggtgg ggtccaccac tgttttgcca tcctgaagtt
   124681 ttccgccaat aaaccacaca acaagcccac cgctggcttt gctgaccaca ccgccaacat
   124741 agtggagcga atcaaaccca atctgattcc acgaaggaag cattgttggg tttggcgcag
   124801 ctaatcgttt acattcaaac actgtctggg gtttcccagt gatttgtgga taggtatatg
   124861 gcaaaacacc tttatatgga gctatcgcaa atgaatagga aacatgaaaa gtgcctgctt
   124921 tgctaccccc aaaaaacttg agcccaaacc tgtgcatgcc agtaatatat tgtcctttga
   124981 tggtgatggt tatggtgcca ccatcctggc caacccatgt ttcctgcggg gtaatgacaa
   125041 agaactgttt gtttgcagat acgtcaatac gatgttttgg atttcctgtt attgatactg
   125101 aaagtgaatc cctgtcaata cttgatttca gtgtgtcacc atttttgcgc acaaagaggg
   125161 taaaggcaag tggctggtta gcatcaatgt ttgcaggtgg ttgaggtagc agttttccaa
   125221 acgaactggt atatactaaa aaggcgccat cgttggggag agcttcacct ttgccaaaat
   125281 aacaattttt atctttgtaa gcattgtgta atggataatc ataggggata aagaacacac
   125341 tgccattttc accaccaatg tatacgccgt ttggccccag tgctggagag ctgtttaaat
   125401 cattgcggtc ttcatcaatg cacagatatg accatcgcag ggtaccgtca ggattaaggc
   125461 agaaaagctt gccttcgcca gaaccaacgt atatgatgtc attgccatca atagctgctg
   125521 acgagcgtat gggctctaat gtgtcatatg cccacacaat ctttccgttt tctggattca
   125581 gcgcatagat agtgccatca gcgcatggct ggatgataag cccatgtgaa gtttgagacg
   125641 ggcttgcata gatgtggtca cgcaggccct gtttccacag ctgtttacca ctgtcctgcg
   125701 catatgcgcg cacgtagccg tcataaccac caacgattac catccccttt acattgcagt
   125761 ttgtaagaag tggtgaagct gcattggaac caaaaccgcc atttgtccat tcagcctttt
   125821 gtgtgcgata gttatagcaa tacacattct tgagcgccat aaactgagtg ccaaaaaaca
   125881 tcttccctgt ttttgtgttt acagcaggaa gcgaccatat catttcatta gcagggaact
   125941 gtgttattct ttttccattt tggcggtcaa tctgatatac cagatagtta tcgtttgggg
   126001 caatgatact gccatcagga agcatgccaa tgttgccttc aaaccagttc acgttgtatg
   126061 tttttattgt aaactgctcc cacacttcct gaacactgtg agctttactt ttccatacaa
   126121 tgaagccagt ttctctgttg atacaataca cataactatc gccactacca acatacacaa
   126181 accccctgtc atcaagaagc gctgatgaat caatgatctc ccctgttttg attttccatt
   126241 taagggaacc atcctgcttg atggcataaa agtaatggtc agcagaacca atgtatacag
   126301 taccgtgagc atcaacaact ggtgagctga atatgccttt acctgtttta aatacccacg
   126361 gacgcagtgt ttttctatac gttggtttca cggcgcttct tccgttttga aggctgtttg
   126421 ctctgaattt tggccatggg ctttttgggt caaggggaat gtcgtaggta tattttcctt
   126481 cattttgggg cgatagtgta tcgggatagg cctggtaggt gccaaaggga tgggttgcaa
   126541 taaaataata taatgctgca actacaatga caggatatac aatatggcgc tttttaaatg
   126601 tgtacgatag tttcattggt ttaccttgtt aaatatatag tttataatga aaaatttatt
   126661 aatactaatg acctggacga aataccaaaa taacttcttt ctgaaaatgc aatagcaatg
   126721 tatagctata ctataaataa atacctcata aaaataacta aatagtcatt tatttatttt
   126781 gtcaacacaa aatttacttt aatggtattg atttttatgc catatatact cataattgaa
   126841 atttgttaac aaagtaataa ataatacaca acatctctgt gtttagaaag attttgcatt
   126901 aatatcataa taatggtgta tggtagtata actgtgtttg atgtttgcaa tatgaaaaaa
   126961 aggaaatatt ttcattgcaa gttatcatga actactcttg tattgtgaca ggttctattg
   127021 gggaattcac ggtatgcaat ggattcccag atatgttggg aaaacatata ttaattatat
   127081 ggtagttgaa aaaaatggaa aaacagcgtg cgcgcacagg aagtgaaaag gaataccgta
   127141 aagagcaact aaaaaaagct gctctaacat tatttgcaca aaaaggtttt aaaaacacca
   127201 cagttgagat gataacaaaa aaggcacatt taagtccggc ggcattttac ctgtatttta
   127261 ataataaaat agccatatac cgtgcattga ataaggatgg cattgatatt cttgaagatt
   127321 taatgaaaca agcattacat acgtcaggtg gtagcagtgt tgataaacta tatgcacttg
   127381 ccagagcata ttataatttt tacagggaac atacggatta tttttatata accgagattc
   127441 ttcatcttgg caatgatgag tttttttacg atagcgcaat ggtaaaggag cttgaaaaac
   127501 gtacgcttgt attattgcaa atagttgcca gtgtcattga agaaggtata gaaaagaatg
   127561 aattgagaaa gattgatccc atgaaaacag cggttactct atggggtatg atagacggtg
   127621 tgcttatttt agaagtaaag aaaagtacct tatttaccgg atttacgata gatgcattaa
   127681 ttggacaact gatggatatg gtattgctgg gtgttaagaa gaaataaaaa agttattgaa
   127741 aaaatatata cataaaataa aaaagcaaaa atttgttatt taacaaattt aaaaaaatta
   127801 taattaactt tttatcagga ggattttttt atgaagcttt caaaactttt ggttgcgatt
   127861 gcgctggttg ttggactggc agtacctaca tttgctgcag acagcattgc aaagcttacg
   127921 tttggggacg atcagtattt agaactnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
   127981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
   128041 nnnnnncttc cttttatgca tcatgaccgt cagtcagcgg taacattact gtgtgctgat
   128101 tatactgctg ctgttgttcc tgttggcagc aatgtatggc gtgatacagg tattgaattc
   128161 aggggattgc ttgccaatgg acttgtagat taccgcattg gcgttttcga tgggaaggat
   128221 agagatgctt ctgtaacacc tgttattaat aaaaatgaca tgccacgatt taccggacgt
   128281 attcaggtta acctcaagga tgctgaggaa ggattctttt acagcggtaa ttacctgggc
   128341 aaaaagagca ttataagctt tggtgcagga ttggattatc aacaggatgt atataatgct
   128401 ggcacaaagg caaaagatta taccgcatgg acagttgatg tatattttga ccatccggta
   128461 gccgcaaaca atgtattgac cctccagggt gcatatgttg atgtaaaaaa tacccctttt
   128521 gcatcagtaa ccgttggcgg cacacctgtt tatccggaaa aaatgaagat gtattttgct
   128581 gaaggcggtt tcctcatcaa tggaacatgg cagccagttg ctcgttacta ctataaaaag
   128641 gttgaactta gtggggtaga tggcaaaatt tcagaagttg caggcggatt gaactactac
   128701 attaaaggcc acaatgccaa tataaaagtt gaatatgctc agcaattaaa agatgacact
   128761 gatgacttca agaacaagca gataaccgta cagtgccaga tattcattta attgcaatag
   128821 agcgatggta aaaaaaaggc tgtgcaatgc acagcctttt ttatataatg cccaaatagg
   128881 gaaagtattt ggggaagtaa tgagattgtt agttctgact ttattgcaac gcataatacc
   128941 agtaacgtat agtagttttc ctgagttcaa ggcaaaggat ctcaattata tacaatggta
   129001 gcagttgatg attctatatt tatgagtaca ttggtaggtg gcttttgagt ggtctggctg
   129061 acatggcaac aacctgctat atgtctgttc ttatgttcat caaagggtgg tattggatgt
   129121 aatcgatata atcagtacca tgctccgata agctcagtaa tgttgtattg ataagaaatg
   129181 ccttagaaac tatatggatg gtctttgagt aaatcagcga tttgtttgtg agtttatcgc
   129241 gggaacctat cagtctatca ggatgaaatt tatataaagt ttaattttca caggagaatt
   129301 tttttcgatg ttctttacca aaagaaaata tggatttaca gcaatgttat tcagggtaac
   129361 tatcgcatgt agtgtggtat tgatgctgta ccatgaaaat gcatttgcca gacgaaagga
   129421 tgaggtgctt actatcccac gcagtgaagt aataaaggct tcggttgatt atattggcat
   129481 gacagatttg ctggcaacta ttaatacgtt gtatcttaat ggctattatc atttaatgaa
   129541 acggcatata aatgaatata ataaaaagtt cccgcccata aaggaaattt attatttcac
   129601 tgcgattaca cttatcaacc agaaaaagta tgaagatgca ctgtcgcgct tgtatatagc
   129661 acttgctctg gagccagcgt attcgcgcgc gcttaatgct attggttatg tgtatgcact
   129721 gcgcaggcaa tggcagcagg cactatcgta ctttttactt gctcatagag caaatacata
   129781 caatgcgttt atcagttaca acattgcgct tctgtactat ctcaacttag attatgcaca
   129841 tgcagcagcg tgggcaaaca aagccataga cgacaagcca aactttgccc gtggatgtga
   129901 tgtgtatgca tgcagcatgt atcagcagca aaaatatgat gaggcagtgg aatactgggg
   129961 aagggcactg caactggggc tggatgaaga tagagtgtac tacaacagag cctgtgccta
   130021 ttttgcaatg ggcaattata ataggtgtat tgctgattgt actaaagcct taaagaaaaa
   130081 caaaaaaaat attaatgctt taattctttt agcatttaca cactttactc tgaacgatta
   130141 tgatgcagcg ttgtcagcat gtaacaatgc gttgaagata caaaataata ctattgttgc
   130201 tgctgtactc agggcagtgt gtgccggtaa aaaaaatgca cagataggga aagatatgct
   130261 tgttgcaact cttggcagtg tagagaatgt acaagatgtt gctatgaagc ttttgcaaaa
   130321 ttttcaatat acaattattg attcgcctgc cgattttatg ttatattaaa attaccgttg
   130381 ttttatctgt tcgtagtaga ttgaccgcag tgtttcaaaa aattgaagat attctttttt
   130441 acggtaatcg ggatatgtcc attcccaggg ctggtaatgt tttgccacat accgatattc
   130501 attttcaaca tacacgcctt tacccacata cacacgatga aaataatcct tgcatgatgc
   130561 caaaaataca ttggaaaggg taagatagcc agggtcaata tttatggtgc ggttgccatt
   130621 gtgtaaaaac ttagtttcta ctttattggt aaatagtttt atctttgcta tatcttctct
   130681 attaataagt ttttcaaagc ttatgaatac tttgtataaa tttttcccaa ttgtactgta
   130741 gtaatctgta tgtgaaaagg gtattggttc tgattgtaga tctatggtgc caaatttttt
   130801 ttgtaatata ttgatgctat gaaacaaaac atcatcgcgg gcggttaata gccctatgaa
   130861 cagtttagct ttgggtggta ttgttggctg tgccattggt gctctctaat tgtaataaaa
   130921 atgtacgcat atcctgtggc aatggcgctt ttattgcaat catcttttgc aaaaatggat
   130981 gatataagcg gtacatgcgc gaatgaaggg cacagcgggg aaatgggcgt tgtatggtgc
   131041 cttttgaatg tatgtattca aggaaatcgt catcatggtt acagtacagt gtatcgccag
   131101 ctattgggtg gcctatgtgt tcacagtgta cgcgtatctg gtgagtacgc cctgtttgtg
   131161 gcactgcttt gatgagtgtg taaccattgt atcgtttaat tacagtgaaa tgggtaactg
   131221 cgtgccactg tgcatcggta tatgctgccc gcttttttcg gatacgtgag ttctgtgata
   131281 ttcctatggg ggtgtcaact gttagcctgg taaaggaaca attaccgata actatcgcat
   131341 aatatgtttt tgcgatagtt ttagagtatt gtaaaaatgc tgaggcaatg cgtccgcttt
   131401 tagcaagcac gaccacacct gatgtttcgc ggtctaagcg gttgagtggc atacactgaa
   131461 tgccagtgtg ggaccacaat agcatggtaa gtgtgttgtg gaaatattta ccagcagggt
   131521 gagtgggaag attgccactt ttgttaactg caataatgta atcatcttcg tagagtatgg
   131581 tgtaatgggg gtcaacagtt ggttcttcaa cgggctggcg tttgtattca ataacatagt
   131641 gctgatatac aatataatga gggatagtta tcggtatatt attaacaaag attttaccgc
   131701 ctttgatttc tttttgccaa cgtgagcggg aaagataggt aaaacgtcct gccaggtaaa
   131761 catccaaccg cattggcttg tatccgtcag gaacgataga gataagtttt tgtgttgtgg
   131821 ttgatataat gctgtctttt tccatagctt aatgatgcac gtgcatgcca gtattgtaat
   131881 taaatatact tcattgttgc gtgtaaacaa gacaaatttg cgtatagctt atatggctag
   131941 tataaaaaag tttatggtgc ggcatttgct ttttttaata tttcaggatg tggtccccaa
   132001 taaaactggc aacattgaat cccctcttca aggaaaacaa gaaaactata accactgact
   132061 cgatagttat cggtattatc ttctttttca agggcattga gaaagtcata aagataatta
   132121 tcaatgcagt atgcaataac ctttgccaga gacattttgt aaaccttcct gacatccata
   132181 ataaattcat attcatgttc cagtaacaca atgtgaagcc ttttccagtt acctgagtag
   132241 cgtgttctgt aagacaagcg tgaatatgct tttgtattga tggttttata tgaaacaaca
   132301 tagttaataa aattgataat aaacatactc aaagacagtt tatgtagttt tgcataacgc
   132361 tgaatgatgt caagatgctc gtatgaaatg catgtggtgg tttcgagatt catagtatat
   132421 cctcccaaag taatgctatt tttttaagat taaacagtaa atttttgatt caaaatttca
   132481 actttttttt gaacaacaac ggttttgcga taatcttata aaagatttta ttgttaaatt
   132541 ttttaaaaat tttacataaa ttctattgac aaagtttgac tatcttttta taagtccatt
   132601 cacacggtaa gtaaattctt cccggtggtt atagagaggg ggaaacacct gttcccattc
   132661 cgaacacaga agttaagccc ctcatcgccg atgatactgc accggtaacg gtgtgggaaa
   132721 gtaggtcgct gccgggattg caatcataaa tattattata aaaaagccac tctgtaatga
   132781 gtggtttttt ttattgatac tattaacgta ttatttcccg aaaagcctat tgtaaaacca
   132841 taaaacgata gcatcggaac ggttgaacaa tgaatccctt accttttgca aaggaaatac
   132901 tttatcgttt attacttcaa ttgaatataa gcagtataag gcaatatctt ctccattgac
   132961 aagaataaac aaaaagaatt taaatccttt tttatccaca ataggcatca taaaatattt
   133021 cattacatcc atgttttcag attttaacag gataaattct ggtttctggt atatcgtata
   133081 ttgataaatt acctcaccaa atgttgtatc tttttgcatt gcataaaaag aatattcatc
   133141 tatcaatttg ttaatacaag gatcatgtaa tggttttaat gaaggatatt gtacaatata
   133201 agatttgtca aaaagaatgc gtaatctctt tcttgtttgt gaatagtatt taataccttg
   133261 cattgagcta attgaatgta aaatatttat tataaacaat ctattgctgc cggtcattgg
   133321 gttttttata ttctgcggtt ttttaaacgt ataatatgct tccagtgtaa tgttattcgt
   133381 aataggaata ttttgagtaa taaacgctct tattgcaggt gtagatgtaa gtgtaatctc
   133441 ttgtgttgat ttaagataat gtttttgact atgtattgtt tccggaagaa ttgaataccc
   133501 caataaattt gtttgaggca aaaaaagcaa tataataaat gcaaaaaatt tatatatcat
   133561 atggtggttg cagtccctta taaaatagtt gtgaatatat cctgtataat atatatgtat
   133621 aaatgtatct ttgatttata tagatagcaa atattttcca atacaagaag agtgtaattt
   133681 tttcagtgat ataatgagtt aatactgtta tatttaagct tatatcaaaa ggtattcaat
   133741 aagtgaaaca gcatactatt tactttgcat ttactttttt atggtcatgg agtttatggt
   133801 ctatcccaat ttttttgagt ttgcaagtta cccatcctgt aagtatgtta ttatatatcg
   133861 gtggcggcat agcaccttct tcaacgggga ttattcttgc aaggctcagg ggcgatagtt
   133921 actggaaaag ttttataagg tgttctattt catttttgta taaatttttt tagtgaaata
   133981 atagacattt ccaacgaaac aaaatatttc aggactgtag tgcaattaat gattgctata
   134041 ataattttac ttactaggaa aaaggagcta aagaatgata aaagtatata tagttgatgc
   134101 ctttacatct gaaatgttta aaggaaatcc tgctgcagta tgtgtattgt cttctgttct
   134161 tcctgatgca ttaatgcaag caatagcacg cgaaatgaat tgccctgaaa cagcatttgt
   134221 gttgccccag caagatgggt ttttgctcag atggtttgct ccgcttcaag aggttgattt
   134281 gtgtggtcat gccacattgg ctgcatctca cgtgctttat gaacagcgca tcgcgacagc
   134341 acaacaagag ctaaggtttc ataccaaaag cggtttgctt tcggtaaaaa aacaagacga
   134401 aataattacg atggactttc ctcaggagat agccacagag tattcaattc ctctatgggt
   134461 tattgaagca ttgggtgtta aacctgtgta cacagggaaa aataggatgg attaccttat
   134521 tgaagttgaa acagagaaag aggttttaga gctcaatcct gactttatag ccttaaagaa
   134581 aatggatagt agaggtgcca taataacagc aaaatcttcc agaaaggaat ttgactttgt
   134641 atcaagattt ttttgtcctg gtcttggtgt aaatgaagat cccgttactg gatctgcaca
   134701 ttgctgcctg ggtccttact ggcaacaaaa actacaaaag aatactttta acgcgtacca
   134761 ggcttcacag cgtgggggta ttctaagggt tgaagtgggt gaatcacggg tctatattgg
   134821 cgggaaggca gtcacagtgt taagtggcct cattaatatt taacaatttt tagataacca
   134881 catgtaagca cctgtaattg aatgttggat aaatgtgtat atcatacata gcctttatat
   134941 gcaaaatgga agttgttgta tttgatattt gtatagatag tctagttact ataatcttca
   135001 tgttcaaagg agtgtttaat gcctgcaata attgtagata aagaaaaatg caaaatggac
   135061 aaattgtgtg ttatggaatg tccaatgaag ataatttcgg ttgatgatag tggtattcca
   135121 cagacactta atactgccga gtctatgtgt attgaatgtg gacattgtgt tgcagtttgc
   135181 cctcacggtg cattgagtct accaatgcta agggctgaag agtgccagga tattcattac
   135241 caatggaatc cgggtgttac tgtaatagaa aattatttca agtcaaggcg ttcaatacgc
   135301 agatttaaaa aagatatagt taaaaaggaa gatctgatga aacttctgga aataaccgcc
   135361 tatgccccaa caggccataa ttcacgcact gttgaatata ttgtgtatac tcaaaaagaa
   135421 gaaatacatg cattggtcaa acatgtaatt gattggatgc atacgataat aattaattcc
   135481 cctgaaatgg caaagagcat gcattttgac atgataacaa aagcctggga agatggtata
   135541 gatacagtaa ctcacagtgc gccagtaata atagttgctc atggcaaaaa atccaatcca
   135601 aatactccaa catcatgcac tatagccctt acgcatttag aattaattgc cccggcatta
   135661 gggcttggtt gttgttgggc aggatacttt tcatggtgcg caatggtata tcatccctta
   135721 aagaaagctt tacaaatccc agatggtaat actgtgtacg gaacaatgct ggtggggtat
   135781 ccgttggtta gatactatcg cattcctaaa cgagagcctt gcattgaatg gcgataaaat
   135841 aaccttctta aaaattatta aaatatttat gcaaaataaa aaagcaggcg actcaacgcc
   135901 tgctttaatt ttactttgat gaaattgaat tatcgctcgt aagctccgcc ttcaacttct
   135961 ttctttaaac cttttttctc aacctgatat acgatttcac agttatcgtc atcgtcatag
   136021 gtttctttaa cgatggttcc accttttaca aggccaccaa attcttttgc aactgcaaca
   136081 ccggtagatg cataatcagc agctcctgca gcaccttcca aacgggcacc aacaaacttt
   136141 tcaattattc gcttttcagc cataataata gctgcttctt ttgctgttcc gcgtcgctgg
   136201 attttatttt ttaatcctgg ttttggtact cctgtggcag ttactctgaa aatatcgggt
   136261 gttacccatc cttcggatat aaacgtttca ccttttacct gaccacctat cttttgtgat
   136321 ccgcatgctg taaagctaat tgctaccaca gcaattaatg caactaataa aagctttttc
   136381 ataaacagct cctccttctc tttagggggg ttaccccata ttatattatt aataaactaa
   136441 gtgagtatag ggacatacca tatacttaaa gcaaatagta ataaaaataa aaataaacgt
   136501 caacataaaa aataaaattt ttaggtatta aacccctgta ctcctcttag tatattattc
   136561 aaataatata tgatagtaca aatattttgt ataatagtgc aaaaaaaatt tatatgtttt
   136621 ccttatccag cgaagccagc atgaaaaaca tgatgataca tataataata gcattaacca
   136681 taaaccataa aaatgaaagc tctttttcag taacccaggt atatgtcagt gatgacaaag
   136741 taccagttga aaagaagaat aaaataattt catagtattc agaaagggct gtagctaccg
   136801 tatcaacaaa tgaatcagat accgtaacct tcattggttc gtacgacctg cgtatctgtg
   136861 ggagtataat gaataaaaga atgcagaata cggatgactg gaaaaaccag ttagttgata
   136921 gtaacgggat taatatgcca aacaccagag aggtcagtgc atataccggg tataatctga
   136981 atttggataa tagtggtatc cagtgatctg gaataaggaa aactacctgt tttaattcct
   137041 gttttgtata ttcatccttt gaattaaaaa gtttatagta ttcatataca ataacaatga
   137101 ggattgtata aaaaatacaa agtaacatta tagaaagtaa ccccatacag ttcctccaaa
   137161 aatatagttt atttagcata atttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
   137221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
   137281 nnnntatact gtatagtgaa attttatgca agtattttat ttataaagga tacgtacttg
   137341 caacgatacc attaacagta actatcgcac aataaaaact tttcttcctt tcactttaag
   137401 tttatccata cagtaaagct ttactgattc aatcagtatt tgatgctctt ctttaaggat
   137461 acgtgctgag agtgtgtctt cggtatcatc gtcatatact ggcacagctt tttgcaagat
   137521 aattggcccc gagtctgttc cctcatcaat gaaatgcgtg gtgcatccag aaattttaac
   137581 accatactca agtgcctgct tctgcgcatg agtaccaggg aatgatggga gtagtgcagg
   137641 gtgtatatta ataatcctgt ttttaaactc gtttacaaaa aatggagtta aaaggcgcat
   137701 atagcctgct gttaccacca gatcagtgtt gtattttcgt aaaagctcaa tcatggcttt
   137761 ttcatggtcc tgacgtgtgg gataatcttt tggattaaca aagaaagctg gtattgaaag
   137821 ttcacgtgca atgtccagag cttttacacc tggcttatcc gaaataacaa ggcccactgt
   137881 tgcaggaata tcacctttga taattgcact ggcaacagcc tggaaatttg acccacgtcc
   137941 tgatactaaa aatgatactg tttttttcat atgccctctc atgatattct ctataatctg
   138001 gttgtgataa acacatgcag catataccgc ttcttatcct ggtatgatta gatacgcatc
   138061 tttgataatg ttgcatcgtc aattggatta tctgtaacta atgttgtagt gtggtgcccc
   138121 cgtataaata atgcaacact gttttcatct ggtgatggtt tgccatatcg tgccagtatc
   138181 tgtacaagta ccgggtagtc actttgcagg atagttcctt tgccatagat gacgggccct
   138241 ttaaatgatg gttccacaaa aaagtcggcg gtttgtttgt agcgctgcag tatggtgcat
   138301 tcgttttcat tacgtgctac tattattttt gcatgttcat gtaagcgata gtgcctgcca
   138361 acattggcca gaaaaaggtc taatgcatta aatggcggat gttctattaa gtccttaaca
   138421 cgtgaagcaa gctggggcat ggttaaaaaa catccacctg ctggtgattg aaactgtgtt
   138481 ataccaaatt cttgagctaa ttcaaattgc gtatcgcgcg aacgcccggc aatggcatag
   138541 aggcggtttc tatcaacaat accttccagt tcaggtatgg tgggtggtaa aagttttgcg
   138601 gatagtggtc gcagcaagag gccttcaagt ccacattctt tttctatatg tttgagcatt
   138661 gatttatact gtgacatggg gcgctgacca acaacctctc ctgtaataac aaaggaagca
   138721 ttaagctttc ccatgtattc tttagctttc tgtaaaaaga agattttgca atcaatacag
   138781 gggttagcgt ttttaccgta tccatgtctg ggatgcgcaa taacatttgc atattcatca
   138841 tcacatcgat aaaacacaag aggaagattg atctgctttg caagctgtga ggctttaaga
   138901 ctatcagggt ttattgcagg gtccacagaa ggcaaaatac aatggagacc aataacctca
   138961 atgccctgtt tttgaataat ggcacaagct aaaaggctat ccaggccgcc tgaatatagt
   139021 gctactgctt ttttgttcat agattacact tcgggatgat gtataagctg aacatcaacg
   139081 tgttcacctt gtgaaacact gagcctgtca ggtggcagta taatgaggca atttgccagt
   139141 gccatagaac gcaagatccc tgaaccctgt ggtcctgtta tgcgtacaga cgcctgtcca
   139201 ccttcaaacg atactatccc tcggatgaaa tgcgttcggc catcgttttt acgctttata
   139261 ggttcctggc atatagcttt aataagcggt ttttggagag cttttgcacc ctgcatggcg
   139321 agcatagcag gccgcacaaa ctgaatgaac gacaccatgg acgatacagg attgcccggc
   139381 agtccaaaat acaatgtgtt gccccgatgg gcaaatatgc aaggtttacc tggtttcatg
   139441 gcaatgcgtt caactaaaag cgtaaatccc agttcagcaa tagcactgat cataaaatca
   139501 tatttacctt ctgaaacgcc gcctgaagaa ataatgatat cgcacgatag tgcctgtgtc
   139561 agtgcttctt tgcttgcttc catgctgtca cgggcaatac ctacataatg cggtatgcca
   139621 ccatattcct gcaccagtga catcagcgtg tatgcgtttg aattaatgac aatgccttct
   139681 ttaagggggg taccaggttc gtaaatctca tcgcctgttg ccagtatggc aacacggggt
   139741 ttttgggcga cagttactgt aacattgcct gttgatgcta aaagaccaac gattgcaggt
   139801 gtaatttttg tcccccggct aacgatacaa tcaccttttt tgatatcttc accagcctgg
   139861 cgaatgtttt ccccttcttt tacccgacga aatattgtca cctgattatt atcttctgtt
   139921 gtagcttctt tttcaactac ggcatcagca cctggaggta tgggagcacc agtcatgatg
   139981 cggatagcct ctcccgactt tattggtatg ggattgtgaa taattcctgc ctggtattca
   140041 ccggcaatgg gcaatgtgac aggattgtta tttgaggcat tgagtgtatc actggctact
   140101 atcgcatagc catccatggc agaattggtg tgatagggaa tattttctgt agcatacata
   140161 tcatcagcgc agatgcgacc tgatgcattg gtaaagtgaa ccttttcaac tggtaatggc
   140221 tgtatatgtg acagtataat cttaatggct tcatcaggtg taatcatcag tacctcccag
   140281 aatggcaaaa taaacactgt ataagtaaca gtatactgct ttgaatggtg tttacaattt
   140341 tttaaatgat ggtagtgttt atatcatatt aatggattgt atgcgtcaag gaatattatg
   140401 taatgaaagt catacctgat taaatgattt ttcatgaaaa gggaatttga atcaagatat
   140461 ggttttggaa aaattaaacg agaatgcatt accgttttgg ccagaaatat ttttcatgat
   140521 taaaatttgt aatgtattcc atgcaatcac tcatgggcat aggctttgaa aagtaatacc
   140581 cctgagcttc atcaacacct aaacccgtaa ggaacgccag ttgttctctg gtttccacgc
   140641 cttcagctat tgttcgtttg cccagtgact gagccagaag aacaatagtt ttaactatct
   140701 ctacatcatc cctgttatca ggcaatccct gtacaaacga tgaatctatt ttgagggtac
   140761 tcaaaggtag tttttttaaa tatgcaagcg acgaataccc ggtaccaaag tcatcaatgg
   140821 caatattaat accaaattgc tgcaaagcat tacaggtatt aatgacttct gctgcatcgg
   140881 acataagtaa attttcggtc acttctatct gcagatgtga agggttaatc atggaacttt
   140941 ttacagactt tatgacatca actataaaat tggaatcata aaattgtgct ggtgatacgt
   141001 tgaacgaaac ggtaaatcca tgctgttgta attgtggagt aatttgcacc tggcggctta
   141061 cttcggcaat aatccacctg ccagcctgaa taatgatatc tgatgattcc agtacagaaa
   141121 taaagtgtga gggtgccagc ataccccgtt tggggtggtg ccagcgtata agggcttcca
   141181 ggcctgaaat ctgtcttgta gtaaggttaa ttgttggctg ataatataac tcaaattcat
   141241 tattttttaa tgctgccctg agttcctgtt gtagtgaaat cttttttata tttttctgga
   141301 atagttgttc agaataatag catactgtac catatcctat cttcttggct tcagaaagtg
   141361 caactctgga tttatgtaca acatcatcag cattgtttcc atcacccggg aatacagcaa
   141421 taccgataga atacgttacc atgaggtcat tgcttatatc atcaaatggt gtcattgaca
   141481 atcgtttgag gtcatcaata aaattgatca attccatatc atcagtaaag gggaatattg
   141541 tatagaactc atcactactg cgcgccacca ggtgtgaatt gtgtaacagc tgggaaattc
   141601 ttttgccaaa ttcctgtaat attttgtcac cctgaagata gcctattgaa ttattgattg
   141661 cagtaaaatt gtctatgtca atggttacta ttgccagttt aacattttgt tgtgatgcaa
   141721 ctttcatctg tgaagagagc attgaagtaa agtaatgtac attacctaat ccagtaaggg
   141781 tatcagtaaa aagtatagcc tcatattctt tatctcttct gatattgcgt atggcaaagg
   141841 acaggtcatt tgccacttcc tgaagcattg ctgcttcgcc aagtgaaacg aaattttcat
   141901 gacatgaata aagtgtaatg gcacctattg catcatgttg ttcatcaggg acgataggca
   141961 tagaaattat tgatttaaat ccgtacttat gtgccctttc tttccatggt gcataccgtg
   142021 gatcttcatc agggtaattt atccgaacgg tagttttttc tcgtattgca gttcctaccg
   142081 ggccgttacc ttctggaatt gcagggtcaa ctgatataaa aatattatca agatattcag
   142141 tttttccaca agaagctacc ggtacaactt tgaggtcatt gtcaacaata ccaatccatg
   142201 ccagatcaaa attgccaagt gcatcaagga tttttacagt attttcaaac agggcttttt
   142261 catcatttgt acggatgata aggtcattga tttcatgtat taaccggtat gatctggtta
   142321 catgagcaag ttctttcttt gattctgaaa gttttttatc ctttacaaaa aaatatgcat
   142381 aaaggatcaa tgctaaatca agaaaaacta ttttcatata tgcctttact actggcgcta
   142441 acagtgaatc atcgtttatg ctatttttta tgtattccag aagataggaa tatacatttc
   142501 catatgaagt tgaaaagaga attggattaa catcatattt ttcaagttgt attccaacct
   142561 ttagcctgtt taatatataa tcattgtcag gtatacctga tgcaaaacgc ttaaaataat
   142621 cattaagagc ctgtgtgtga tttttaataa atgaaaagtt gtcaaaaaat ttcttgaaac
   142681 tgtttattcc ctttaaataa gcatggaact catcccacaa ctgatcaggt gcattggtga
   142741 tcaattgttg gattcgggta ataatttctt tatcctgttc atcaattgat aaagaatgct
   142801 gcaaattttt aatgaatgct tcatccagat ttagattctg acttataatg gtaattaatc
   142861 gatcaagatt tttcatataa ataaaattcc gtccagactt attatttata gtataaaaat
   142921 ttttgctatc attgtcaata aaaaatatat ttttttatat tttttgtccc agaacataat
   142981 tacctccaat ttacccctgc aggcattttt atataatctt ccctgcatag ccagtatttt
   143041 tatgctatta ccagcatata tattttcagt ccttcagata aagatatgtt aatcttggat
   143101 aaaatttatg tttcatataa aatgcaaaca ctgattcatg tccttcggca actgaaacca
   143161 ttatttttgt gcaattatta tcttttaacc atgtaactgc attttctaat aattggctac
   143221 catacccata tgttctaaaa ctttcttcaa taaatagtga atctatttcg ccattaccat
   143281 tctggattgt tgcaatgcaa taacctatta atgttttttc atcttcgatt gtttctattc
   143341 gtatattatt atcatcaatt acgttaaatt tttcacaacg tttttcaaat gtaaaggttt
   143401 catagtggtc tttgaaataa tgtgaatcat ttaaatggat gatatttaat ttttcccata
   143461 attcttttag tataggtaaa tgatttttac tgacagttgt tgcacttatc tcatggtcca
   143521 atcttctatt tttaaatttt ttactttacc aaattctttt gtaatatgtg tcacaagaat
   143581 tccgtttttt gctaaaactg ttgcagcaat gatataatcg tttggtccta taattgttcc
   143641 tttcaattcc attgctgatc gaattttaga ataaataatt gaacaatcat tatcgaatgg
   143701 gattatttca aaaggttcta gaaatcggtt aataatagac aaattttttg ctttttgctg
   143761 acttttttcg gctccgtata gaagctctgc ttttactatg gaagggatct tgatattatt
   143821 tggattagta ttttgtatat ttttctttat tgaaggatat aatcctttaa gaaaatatac
   143881 acagatatta gaatcaagaa aatatatcat aagctttctc tttttaaatc ttgatcaaat
   143941 gataattcct ttatgtcaaa tgatttatct gttatggagc caaataaatc aaaatatcct
   144001 tctggatact cttcctgtat aacttttttg atattcttgc ctacccattt tgagattgaa
   144061 atgcgctctt tagctgctgc tttttcaatc ttttccagag tttctttgtc aatatataat
   144121 gatatttgtg gcatagtata cctcctgagt aaaatataat taactggtag atgtaagtca
   144181 agtatattta caagtatatt ttatggtgcc acttactttt tgaatacgtg catcgttgcc
   144241 agaaaattgg aattattttg gtaaacgaac tacttgtctt ttttaataaa tcaaaaattt
   144301 tgcactatgg ataacaatta cttcaactat ataataatat actctagtat atattttgtt
   144361 aaatcgtaaa gcgtaattgt ttattacatt taatcgtata gggcacatag atatatctat
   144421 aatttagagt gcacccccgg agtataggct gttgcagaag atctcagtaa aaacttatta
   144481 caccataatt tttacttttt aaaagatcat ttattaattc catgtcaaag tattctggat
   144541 caaactcacc cccaagccat tccatcattt catcatattc ttcattctct ggatcagaaa
   144601 taattttaag caaatcaagg tagccatacg gacctccaca atcttcaggt gggcaattcc
   144661 ttttaccacc tatgcaaact ggatgttttg ggaaatcacc ctctgtaata attttttcta
   144721 aaacaatttt atgctcccag ccatcgccaa aatcatattc atatagtatg tattgatttt
   144781 ctttttttag taattgattt aattttatct ttctataatc tacatattct attaaagaat
   144841 cttcatcagg ttctgaataa attatatcat ctttaacaaa ttggtgtaaa tgcgtatttg
   144901 tccaacccat tactgtttga attactttat gcaaatctgg aagtttaatg tctgaattaa
   144961 caataaacct cctccatatt aatggtttga tatggagaag gtaaattttt aattgatacc
   145021 atttgttgct tgtatgcttt gctgtttttg ttgtcataat tgataccctt gatatctggt
   145081 taatattgaa attattgata tcagttacaa ttttggtgta tatgttacat tgtgatcacc
   145141 ttccagggga aggttcctat gtaaactaac gtaatgaagg cttggtaaag ataacatata
   145201 tcttcgtgat aagtcaattc caattttgtg agtaatcgta tcagatacca gatcatgtag
   145261 taaatagtct tacaaaataa taattcatat atataccata ttatgtaaaa tggtatttat
   145321 tctacaaata tttgtttctg cggattatta atacataacc ggaaatacat taaaaataat
   145381 ctcttgttta tgttcaccct ggttttatcc atcttttatg aacatggaat catcagggaa
   145441 atcattcaat ccataaagat ggtactaaag aaatattaaa tgaaaaaaat caataaaaaa
   145501 gtgacaactt tattgacata tagcataatg aaataaataa caattaacaa tttaatacta
   145561 tttttcagct ttattacaaa aatcaaaaaa acaatattaa tgaataataa tttcaaattt
   145621 tattattaca aattatcaaa atatatatta gatttgatag aaaaagcaaa aatgaccgaa
   145681 cactcattta tggttattat tgctttgatt attggcattt tttcaggctt cagtgccata
   145741 gccataaggg caatgattaa aggtttttcg cttgtatctt tcccaggaac acaaaacata
   145801 ctggagaaca taaaagctac tccatggttt aaaattttat ttatacctgc cattggggga
   145861 ttaattgtag gccctattat atattttttt gcaaaagaag caaaagggca tggagtccca
   145921 gaggtaatgc aggctattat aaaacagggt gggataataa ggccaagggt tgctataatt
   145981 aaagctcttg cttcatctat tacaattggt actggtggat cagttggacg tgaaggacca
   146041 attgtacaaa ttggtgcaag tcttggttca tcaattggac aatttttcag ggtatcatca
   146101 accagactta aaacttttgt tgcatgtggt gccgcatcag gaattgcagc agcatttaac
   146161 gcgcccgttg ctggtgctct ttttgcagtt gaaattattt taggtgattt tggtttaaca
   146221 caattttcac ccatagttat ttcttcagtc atggcgactg tgatatcaca tcaattcgaa
   146281 ggtaattttg ccgcttttga ggtgccaaaa tatcagctta aaactcctta tgaactcatt
   146341 ttttacttta tgctcgcttt tatatgtgca acggttgcct ttttatttat aaaagtgctc
   146401 tatttttttg aagatttttt tgaaaacaag gttaaaatac cagagtattt tcaacctgct
   146461 attggtggac taattattgg tttaatggct ttaattttac ctgacattat gggagttgga
   146521 tatgatagca tcaatgctgc tttgcatggc aatatgatct ggtatttttc attcatttta
   146581 atatttttta aaattattgc aacttccata acacttggtt ctggtggatc aggtggtata
   146641 tttgcaccat ctttattttt aggcgcaatg actggtgtaa cttttgggaa tttaattcat
   146701 tacttttttc catatattag tgctacaccc ggagcttatg cacttgtagc aatgggagga
   146761 cttgtagctg gtgccacaca tgcaccaata acagctatta tcattgtatt tgaactcaca
   146821 aacgactatc atataatatt accgttgatg attgtatgta taatctcaac ccttgttacc
   146881 caaaaattat cacgtgaatc aatttataca ttgaagcttg ttcaacgcaa tatttttata
   146941 aaagatggaa ctgatatcaa tataatgagt tcaatatatg ttaatgatgt atatacaaaa
   147001 aatataagca aaattccatt ttacactaca tttgatgagg ttataaacaa acttatcgca
   147061 tcacatgatt catatatagt tgtcgttgat aaaaaagggg cttatactgg tattatctcc
   147121 atgcaggata ttaaggataa tctttttgac aaagatgttt tacaatcact attaattgca
   147181 agtgacatag ttaataaaaa tatacctact gtgaacccca cccataactg tcagtatgtt
   147241 cttgatttat tatcaaaaca tgatattgag ggattgccag ttattgacga taagtcaaaa
   147301 aaaatattag gaataatctg gcgtaaaaat attttagatg catataataa agaaattgag
   147361 aaaagggata ttactacacg atttgcaaac aatatcataa tgccacaaat tgaccaatcc
   147421 gtccattttt tggagggata tgctattacc gaaattcctg ctcctgaaat gtttattgga
   147481 aaatctatta aaaatctaca aatacgatca aaatatggat ttgaaatact gttaatacgc
   147541 tccaatactc cacatggagt gaaaataaaa gccataccaa gccctgatta tataatttca
   147601 atcaatgatt cactggttat tgcaggtgag ataggaaata taaacagaat aaaaagcagg
   147661 atataatata taaattatat ccaataatat attacataaa atattatgct gtatatataa
   147721 tcatgctcca attttattca tttttttccc gctgtgttta tccagaattt tctcttatat
   147781 ttttaatgtt ttatgaaatt ttttaaatat caaatgtttt atccgggttt gtttagaaag
   147841 cattttcagc aattgtcagt atcgatgtgt caccatctcg gtacctgtta gatttaatta
   147901 aattcagacc atagtgcttt gctgtatttt gtgattccgt taaaactgaa tgcaaaatct
   147961 ctacactact ttgtcccaat tgcctgtttt tacaatcagt tttatttcat aatcgaaagc
   148021 atttttgtaa attcacactt gaaagacact cttataaaaa aaattactat aaaattatat
   148081 aatatcatac agaccttgaa cgctggagtg cctataaaac tcttttaatc cttcactgga
   148141 ctgaattaca taagatatat ctttataagt tgcagaaaat ctatggcact ttgacaaaag
   148201 aaaagaaaaa tcaggtcaga aaactgatac ggtgtggtaa tttttaaaat ggatttcaac
   148261 gtcatacctg tccaaactgt agtatagttc tagttgtgcc ctttacctgt ggaatgttgt
   148321 attttcgttt cttgctgata aagagattaa aaaactagtg aaaacttatg gtatttataa
   148381 gggatatttc atacctttta gtaaatcaag acctttatga taaaattcat ttttaaagta
   148441 gcgtgtatca aaatagagct tgacgatttg atgtgttttc agtatttttt atcgacacga
   148501 cagatgaggt atgtctgttg ggaaattttg gtgtgatttt tcgcgcaaaa ttttagccac
   148561 aagctgttaa ataggtattt gcagcttgaa aaataccttt tattggaatg gtttttatag
   148621 agaccttttg tgtaatgagc ccgtctttat ataagatcat tttcctaaaa catcaaaata
   148681 gtggagagtt tatatgaatg ttaattttgg attgttcctt aggtctcttg tagtttcaat
   148741 acttcttggt ttttttgtat cattctttgt tcccggcagc acacttctgg ccgatggagg
   148801 caatgagcct gaaggctatt ccattaatgt gagccataat agcgacacat catttactgt
   148861 agaaaagagt tatcctgaaa gtgctgtact taaaaaaaag taccggtcac aaaaagctgc
   148921 tttcagttat agaatcgatc tcatagttga agatgctggc gatgacacaa tcattggtac
   148981 ccataccttc acttcggcaa taccggcagg gaaagacagc attgtcattg atgtgaaaaa
   149041 atatgttact gagctgaaaa aaaagattaa aatgaggggc cgtgtggcca ttaccctgga
   149101 gactccatcc ggtgtcagag actatgtagc tataggagtg tctgatgcta tcagtgtcta
   149161 taacagtggc tatcaacaac agtttattga taacagcgat cttgccgcac agtggtctga
   149221 agattacaat aattttatta tggaaaatca gggtaaagat atcaatacaa taaacaggga
   149281 atttctgaaa aaatatcctc agtataaaga atttatgccc tatgtgaaat gatatgttta
   149341 gtcttgaaga gtaaataata ataaaaattt tttgccggga aggagatgag agaggggata
   149401 atgttagtta ttactcgtga
//
LOCUS       DAORYL010000002       122346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_96_length_122346_cov_15.086957, whole genome shotgun sequence.
ACCESSION   DAORYL010000002 DAORYL010000000
VERSION     DAORYL010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 122346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 122346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..122346
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96_length_122346_cov_15.086957"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..784)
                     /locus_tag="PLV81_00650"
     CDS             complement(224..784)
                     /locus_tag="PLV81_00650"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HOM08642.1"
                     /translation="MRKSVVVVIALLVAVCMMSDVFSLSLGGKDLVKKGTGKRTKGIL
                     GTVYYATLWVPESLKTADAKAIVEADEPMAVVLVIDSVLLNKERFLEATKEGFAQAAS
                     SGYATAKQDAFLKLFDAVEFKKGDVIQLGYAPKTGITAVFTASATKKTTTLGSVAGLD
                     LKKALFAIWLGPKPVQESLKKAMLGM"
     gene            complement(868..1524)
                     /locus_tag="PLV81_00655"
     CDS             complement(868..1524)
                     /locus_tag="PLV81_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015194725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HOM08643.1"
                     /translation="MQHANTTYCIFCDFDGTITTVETFVGMCKTFAPGLFDEIGKKMW
                     NKELTIRQGVRQLLESLPSSLYSDMLAYIDDKEIRPGFEEFLEYCNNRSIPVIIISGG
                     LYDSVVRKLGNLKNKVTAIYAAKIDASGKYLKVYSDYEHEGELVAKANIMSNYRHTCK
                     IAIGDGPTDIEMAKYADIVFARGLLKKFLPEDKEYYEWDDFFDVMDTLSIIIEQKNFN
                     "
     gene            complement(1553..3139)
                     /locus_tag="PLV81_00660"
     CDS             complement(1553..3139)
                     /locus_tag="PLV81_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOM08644.1"
                     /translation="MKTNYTARQVLEIKSSGTYIIIFIIIAVIAHIFQILGKVDILEI
                     LRLSLISTICVILAYVIYKRKKLLKATGVFEWILGFISVNIPLAAKFAYAQKYDWTFA
                     LESYNSSVLMVILLVMLQLYFNRRLMAFYTGYTVVTWTAFLLVAIAHGAVIHWDATLD
                     GKPVHGFIIFREIFFIMITAVFGILASRNIKVVQQYDDRSTRQLSVIQQQSDMQRQLN
                     ETIKEKVADLLEQVKQQDILTIKFNEKMQNQASTFEEISATLEELLGSAESIARISEE
                     QVGGNEMMETIVSEFKVIKNETRQNLDKTIQEVENVVARTGNANERIKEVEATINQIR
                     EQSQKIGDTINVIVDIADKINLLSLNASIEAARAGEYGRGFAVVADEIGKLAFQTSES
                     IKDIEKVLIQSTKTTETGVEVIRTTAEMLKDLIARMAESSSKIKILQDSVTVEEKYIK
                     NIIDQMEKNVELAKNIGAGTEEQKAAIETSAKAIEHMNEVVAQMVDEIQQLAHTSRSI
                     LDNATALLEKSRGDSSLDTI"
     gene            3604..4866
                     /locus_tag="PLV81_00665"
     CDS             3604..4866
                     /locus_tag="PLV81_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013238147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOM08645.1"
                     /translation="MNTINIATLNKKRYLHVLLPLFVGSIIAYLDRVNIAYAALTMNK
                     DLGFSAEIFGMGAGIFFVGYILFEIPGAIIAEKWNPKGWLMRIMITWGIVSFFMAFIT
                     KPWHFYLLRFLLGTAEASFYPVLYASMIPRWFTPEERPKAIALMLTSLQVSAIIGSPL
                     AGFLLDISVFGLKGWQLLFVCEAIPALLFGILLYFILSNDPLHVTWLTDAEKKYLSDT
                     LSHELKQKGQHYTVWQALKDKEVLKLALIYFLWITGFWGFNYWMPTVIKELSGWSNIA
                     IGLLFAIPMTLSLIVMVCIGHSSSKTGEKRLHGALPLVIASIGLGVGVFIKDPAVNMI
                     FVCLAAIGVFGPFGVWWSYPTTFLSGAAAAGAIGLINSSGNIGGYVGPYLIGIIKDIT
                     NSFKVSFVILACMVLTSGIMMLTLKKKV"
     gene            4869..5387
                     /locus_tag="PLV81_00670"
     CDS             4869..5387
                     /locus_tag="PLV81_00670"
                     /inference="COORDINATES: protein motif:HMM:NF014614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOM08646.1"
                     /translation="MKGFVQCYTGNGKGKTTAAIGLAIRAAGWGKHSIIIQFLKGQKS
                     GEQIALKRFNDLITIETYGSKNFYIPGNNDTEHKTLVHKAYNRCMEVCGDSSYDIIIC
                     DEILTALHFNLIAMNDIIALIQAKRIDAELILTGRGLPKKLFPYCDLITEMKDIKHYY
                     EKGINARKGIEW"
     gene            complement(5440..7422)
                     /gene="acs"
                     /locus_tag="PLV81_00675"
     CDS             complement(5440..7422)
                     /gene="acs"
                     /locus_tag="PLV81_00675"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006892867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOM08647.1"
                     /translation="MAEVTLKEIYPVPEKFRQKAYIKSREEYEKMWKESLANPEGFWG
                     KIAEEYVTWFKKWDKVMDCNYGTTAKDLWVRWFVNAKVNVSYNCLDRHLEKRGNQVAL
                     IWEGNEPTEDKKFTYKELHEQVCKFANVLKANGVKKGDRVTFFLPMIPELAIGILACT
                     RIGAVHSVVFGGFSAEALRDRIQDCKSEVVVSCDGTFRGAKAVPQKDTADKAIAECPT
                     VRLHIVVKRVGDKIQVNWNNKMDKWYEEEMAKVDANCPAEQMDAEDPLFILYTSGSTG
                     KPKGVMHTTGGYLTYVAFTHKMIFDYHDGDIYWCTADIGWVTGHSYIVYGPLANGATT
                     IMFEGVPTYPDFGRFWAIVEKYKVNIFYTAPTAIRALAKEGDSWVDKHDISSIQLLGS
                     VGEPLNPEAWNWYYHKIGRGECPIVDTWWQTETGGILITPLPGAIDIKPAMATVPFFG
                     VEPCIVDDNGTELEGVCSGNLCIKRSWPGQMRGVYGDPERFFNTYFTQFPGKYFTGDG
                     CKRDKDGYYQITGRVDDVINVSGHRMGTAEIESALVAHPKVAEAAVVGYPHEIKGQSI
                     YAFVTLKTGVEKTDELKKELVAHVRKEIGPIATPEMIQWADALPKTRSGKIMRRILKK
                     IAAGEFDKSGMGDTSTLADPTVVDTLINDAKKLQGK"
     gene            complement(7513..8088)
                     /gene="purH"
                     /locus_tag="PLV81_00680"
                     /pseudo
     CDS             complement(7513..8088)
                     /gene="purH"
                     /locus_tag="PLV81_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512841.1"
                     /note="involved in de novo purine biosynthesis;
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
     gene            8623..9195
                     /locus_tag="PLV81_00685"
     CDS             8623..9195
                     /locus_tag="PLV81_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOM08648.1"
                     /translation="MTKRKQQQILTTAEAMFNRFGIKKTAIDDIARDARVAKATIYNY
                     FGNKEGLLKALITQKIQQFESRLLNTAETAINPVDAIKSLIQEHIRLFTSNPFINDPA
                     LQDNELKEELNQKEEHLLQKIVQHASRKGLLQSGDSNQIINTLLLLIKGLESSIKNIS
                     FSMDSLNDSINFISETFFKNTAKEGHNGTA"
     gene            9182..9922
                     /locus_tag="PLV81_00690"
     CDS             9182..9922
                     /locus_tag="PLV81_00690"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HOM08649.1"
                     /translation="MAQLNVMLFEFIVFSVAGWIVEVIYRSLTNKRFVNPGFLTGPYL
                     PIYGTGALIITSIAYATIHIPVVYKFVIYCIALSLLEYITGAILYTTTGKRYWDYTDE
                     PLNIQGHVCLPFSVIWGMLALAFEYAVLPLYGYFVSSLNTVIINTAISLGMLLMITDC
                     VLTHNIVQRLLHARLPVFNPTQYRLGLIKFQNDALISLRGFMDTALSYRKKFSTIKPL
                     NANKIIQLFKKRYTALISTIRNKNENHR"
     gene            9943..10467
                     /locus_tag="PLV81_00695"
     CDS             9943..10467
                     /locus_tag="PLV81_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08650.1"
                     /translation="MENKRLFLLPFVQDILHNEDFQSLRRYTHHGTTTCYHHTLEVAW
                     LAYRLAVALKLDHVAATRGALLHDFFMYNWRTGGPRLHGFRHPRIALNNASKHFVLGP
                     KEKDSILRHMWPLTPLPPRYPESWIVTVADKIVSTMDYFRFVKSKVSLMYNTIHTCMW
                     LPLLSRFNRQKQYS"
     gene            complement(10564..>11625)
                     /locus_tag="PLV81_00700"
     CDS             complement(10564..>11625)
                     /locus_tag="PLV81_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HOM08651.1"
                     /translation="FEETAHKELTLLGIKECAMTKGGVTFRGTLEHCYTANYYSRVIT
                     RILMRLCHFRAYHFNEIYRTLKEFPWELYIQGHTIDVEVSVHKSKLHHTERIGHEVIK
                     AIEKRLSKFAISSNADSVQKVFVRFDHDECMVSLDSSGEPLYKRGYKQHISEAPLRET
                     IAAALLYECNVYDYDTIIDPMCGSGTFPIEAWCMLHAIPPGANRTFAFMSWPSFRKHA
                     FTGITHNESGEHRDITIIAGDSNPDMVAITQQNSMKAGAQLHTYTGDFLKEKKVIGSG
                     KVLCTINPPYGKRIHTDDTIQLYKRLVYIFNKWYPAWSFCVLIPSKIKHIVTIPTDKE
                     IHFSHGGLPVSALIVYRGR"
     assembly_gap    11626..11725
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11879..13018)
                     /gene="rlmD"
                     /locus_tag="PLV81_00705"
     CDS             complement(11879..13018)
                     /gene="rlmD"
                     /locus_tag="PLV81_00705"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOM08652.1"
                     /translation="MKKHRKVLLSIADRFADKRIEPPCMYAGNCGGCALQHVDYSTQL
                     EIKKEYCNTLLNTVATIDAIEPSIPLGYRNRMDYVTAFGKIGLRRRGSFKHVVDLHDC
                     LLLQDKSRRIFAIIREKLSGVQDYDYLVHKGYLRYVVLRQGFNTGQVMVNFVVARQEN
                     NLESVIDAIYNDVDSISLILSDGMADVSYGEVFEDIKKGYIEESLDGIIYRITPNSFF
                     QANTSITQVMYREIAKQVYGNVLDMYCGVGSISLFIAKQAESVTGVEANHEAVTVAGH
                     NATFNGINNVLFVQGDAFEYISDTAVKYDVAIVDPPRTGLGPKVVNKLLQADLKRIVY
                     MSCNPVTFAQDAKMLCDKYAIAWCKAYDMFPQTPHLELLAAFDKK"
     gene            complement(13025..14602)
                     /locus_tag="PLV81_00710"
     CDS             complement(13025..14602)
                     /locus_tag="PLV81_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HOM08653.1"
                     /translation="MKNCMKKTIFTLAILITSITVVYAQIVKPEISNVIVYYDSALVK
                     KEAKVNLHKGENVITIEGLPASMVNESVQVVTKGALLRDVSVQTTYLKKGDVQRINAL
                     KEKLESINNEINAKQNDIQALNTVLEYIKKGSTSPFAVNMTAAQMQSILQLIEQHSSQ
                     CYKKIATLQQALQKHKEEKERLEKELSLLNTDEATKSLQLVVVHTGQDTATVMIQYIT
                     GNAGWNMEYEVRTNTETQTVEVVCNARVYQRTGEDWKDVSLELSTSKPFVATQKPVLN
                     PWYLDVYQPSYYYKESAKSMKLRSEDAAEAPQEAEEMPAMQEERIAFSFQIKGAKTIP
                     SDGNAYKIPVAKATTDCAVRYATVPRVLPYVMLEGEFQNPFDFPMQGGNMNVYLDGKF
                     VNSFNVNKLYVPKDLIPVALGVDESLMVERKLLSKKTEYKGLVSKTKRIEYVYSIKLA
                     NGKKRDVTVTVQDAIPVSQNEKITVTLINKNSIGATIDNEGKATWQVQLSPNQKKELT
                     IHFAVEYPESIQVSGLE"
     gene            complement(14831..15619)
                     /locus_tag="PLV81_00715"
     CDS             complement(14831..15619)
                     /locus_tag="PLV81_00715"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOM08654.1"
                     /translation="MHVLDNDISLVEVSPGKFKGTISQNWLVGGIPNGGYLIALLGNA
                     MAMQSDKRSTPIVTANYISRCKPGDCEIHVECFAQSAQFNRYQASLYQEGKEKIRCWG
                     TFANEKIECDIEKYETPVPQVAPLQECVPIPEVPHFTLSSHVDIRLDPYSAGWMQGKL
                     RDESIQKGWISFRQERVYDIPALLLMSDVIPPAVFVSLGVIAWVPTIELTINIRNMPQ
                     SKWLRFYTRTRFITCGLLETDGETWDENRLVLISRQIAQVRIQK"
     gene            15892..17271
                     /gene="nhaC"
                     /locus_tag="PLV81_00720"
     CDS             15892..17271
                     /gene="nhaC"
                     /locus_tag="PLV81_00720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HOM08655.1"
                     /translation="MKKKLPSFFDCIALLTITIILLFTGTVLFKIPIPLLLIIAATLA
                     GIKSYFLGHNWSEIEGSIVNGIKGTLPAIMILLTVGILSGSFIASGTIPLLIDYGLKL
                     IHPSWILPAAYIVCCISSLVIGSSWTTAATIGVALVGMAGAMHISPAICAGAVVSGAY
                     FGDKLSPLSDTTNLAPGVSETDLFSHIRAMMATTIPAWSLALTGFIVLNIYIVPDTTT
                     RIEHIESLLQTLESSFNLHPVTILPLAVVFIMALVRFPAVVILLSGSTAAILVGWLAQ
                     SQSLLSVLQYAYSGYTIHTNNEIINNLLNRGGIVSFLETTLIILCATGLGGIWESGGY
                     ITSLHDNLLHKIKTSRMLIISTVITAVISNIIMAEQYLSIVMTGRIYKDAYKRKKLQP
                     FMLSRSLEDGGTVTSPLVPWNSCGAFMSSALGVPTLHYLPFAFFNLVMPVVAIVLTAF
                     GLFIYRKND"
     gene            17302..19119
                     /gene="pepF"
                     /locus_tag="PLV81_00725"
     CDS             17302..19119
                     /gene="pepF"
                     /locus_tag="PLV81_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HOM08656.1"
                     /translation="MQKNAKQIPARNEIPAEHTWDLMPLFVSDSNWEEVYSNTEQLIP
                     RYKNYEGKLADSFDVFLQAIEFDLSIARDIDRLYTYAHLRSDEDTTNQNAQAMLQKAH
                     SLAQRAAEASSFLVPEIQSLPEAVISNYLTNTNISQYLFYIEKILRYREHTLSKEIEE
                     ILAMAHEVVIAPMEFFRKLDNADMRFGTITNEQGNTVELSHGNFITFLMSYNQDVRKN
                     AFHTYYKAYDNHKYSIAQSLASSVKKDVFFSRVRKFKSCLHQALFPDNIPDTVYHTLI
                     ETVSSNLQPLFKYLSYRKEALGLTELHFYDTYVPLVADVPFSMEYEEACDVCAQALHL
                     LGEEYISTLHTGLLGGWVDRYENRGKVSGAYSSGCYDSPPYILLNYDANTINSLYTLI
                     HEAGHSMHSYYSRKNQPYVYHEYTIFVAEVASTLNEILLSHYLLQQYSDNPRMQAYIL
                     NREIDEIRGTLFRQTMFAEFELLVHTMAENNKPLTFQTMTDTYTNLLHKYFGTTIVID
                     TDLYLEYLRIPHFYSAFYVYKYATGIAAALSLAKQIINNPDASKRYLQFLSLGGSKFP
                     LEELRIAGVDMESPEPVIDALQYFDSLVDRFIDVHKKIH"
     gene            complement(19116..21353)
                     /locus_tag="PLV81_00730"
     CDS             complement(19116..21353)
                     /locus_tag="PLV81_00730"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOM08657.1"
                     /translation="MISIQKFETWAIKPFFAIFNFISERITYTFAIAFTTSLGGFIIA
                     AYIALFLYATVRFDVSNTGQDFVILFSGMTLFIYAPLYYIHFGLLYNAGVPCFTKRLS
                     LINTYIVHNTLHQTIDDTEAVRLLEALEHLPRDNMTAALIYPSIVMAGVCVYEAIKGA
                     FTNALFLLLGISSAIFIYVFLTYIVAELLSGRMRRKVKWLLVKRGISFNERYILSIRG
                     KFIFINIMVLVAMLEIGLMIYYNNYDIFSPIPILFIVITGFIVFVILFFYLLSIEESL
                     SEIESAAIDLARGGSGSLFLPYLDKELINTSKGFLAAAYEVNDIRRNLEQKVEERTKE
                     LNQALTALKEKNDLIAKELKIAASIQQGILPQTPVVFNAIKVLAYNRASSDIGGDLYD
                     IIRMPNDCLGVLIADVSGHGIPAALISTMVKISFHESCRKYLLPRRIFADVNNQLVNI
                     IRTQEYATVFFMVIFPTYDVMYANGSHRKALVCKRNSGRLEYWDTGGIFLGALPNEEI
                     GGLFEDNFGILEPGDRVFLYTDGLPESRNWNNEEFGDERVEKLLLETLDMPIDDAKEY
                     IIEQWEKFLGGTPPRDDMTFVLLELDPIMHSALQHREKGQELYAQKKYTLAIDELKRG
                     VIMNPEDMTSLLLLARSYIYTRRYSNAVVHLTEYVAKKPDDSMAQYLLAIAQYKLHNY
                     EEAYKAATHSLALRPSYIPALYVMAAICASLHKTSDAIEYCNKILEADPGNNKAQKLL
                     QKLKH"
     gene            21598..23196
                     /locus_tag="PLV81_00735"
     CDS             21598..23196
                     /locus_tag="PLV81_00735"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM08658.1"
                     /translation="MQQISNPKSSIAVLVVDDEESIRTILSRYLALKGYTVLTAPDGN
                     QAMEILHSQPVDLVLTDLMMPNVNGRELLQKMAELFPEIPKIVLSGYGTNEDMLVALK
                     TGAYDFIQKPLTDFEILDKAVERAVESKLLKEERDRYIQQLTYLNDIIGFLNKGVSLE
                     QIFDIIASRMKKAIPFNRLAIALINDAGLVETTLVSSDKPVLIKPGATFPLEESSLVL
                     VAQTKKYDIISDLEEYSKKHNSEKARLLLQEGMQSTMTVPLVVGGRLKGFLLFASCNK
                     NAFAPEHVQFAETISGHIVFSLQRGELLHQLDMYNKKLEEIVKIRTHQLLKTQKATIF
                     ALSSLAELRDPETGYHLERIRGYCMLLAQIAKYTGGFLEINNQFLRDLYDSSILHDIG
                     KVGIPDSILLKPGKLTHEEFEIIKTHTTIGYNSLKRASEEMGRDSFLNMAMDVTLYHH
                     EAWDGSGYPHALSGEDIPLAARIVTIADVYDALTTVRPYKAAFDHQKAIAIMNEESHR
                     YDPKLFELFIQNADDFDEIRNKYQ"
     gene            23217..24065
                     /locus_tag="PLV81_00740"
     CDS             23217..24065
                     /locus_tag="PLV81_00740"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOM08659.1"
                     /translation="MQSYYHFGSAITPSVKKIIIFTSVIYIIQLIGNIITPGLIESTF
                     GIHHIGFIHQLKLWQPLTYMFLHGNFFHIFFNMFALWMFGGDLEELFGSKNFTKYYLY
                     SGIGAGLCIALMNYYIFDSYGYSPITIGASGAIYALLLAYGMIWPEREVLLYFLFPIK
                     MKYLLLIFGAIEFFGTLASASGAGGNISHIGHVGGLITGFILLKSKFWYKRVSLNPFD
                     SLFRNYRLARHKKRIEERIKAKEIIDTLLDKITRYGYGSLSPEEKRQLEWARKHFYPS
                     NNDIIH"
     gene            24158..25453
                     /locus_tag="PLV81_00745"
     CDS             24158..25453
                     /locus_tag="PLV81_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOM08660.1"
                     /translation="MYWNKDIETISRKDLEALQLKKLHKALQAASTTPFYKKVFEENH
                     INIDAIKTLEHIKDLPFTSKNDLRLSYPDGMVAVPRKEIVRMHASSGTTGKSTVIFYT
                     AKDIKDWADLVARCMVATGATPDDVFQNMMGYGLFTGGLGLHYGAELLGCMVIPAASG
                     NTLRQIQLMQDFETTIIHITPSYCLHLVEVIRAEGIDPYAMTVKKAYLGAEPYSEATR
                     KKIEEQFGIDAYNSYGLSEMNGPGVAFECTYKNGMHIWEDYYIVEIINPDTGEVLPPG
                     EEGELVLTHINREAMPIIRYRTRDLTRLIPEPCPCGRTHYRIDRIKGRTDDMLIVSGV
                     NVFPSQIETVLMKFPEVGNNYQIILDRENNLDRMHIKVELYSKLFHGDIKEIERIKNR
                     LKEALKALITINPRIELLEPGSLPPSEGKAKRVIDNRTL"
     gene            25475..25903
                     /locus_tag="PLV81_00750"
     CDS             25475..25903
                     /locus_tag="PLV81_00750"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOM08661.1"
                     /translation="MAFQVSVFAENKPGKIERITSILAKHNINIRAITINDSGDYGII
                     KLLLDRPEDGCTALKEEGVAATLKEIVAVKIGDKPGSLYKVAAILKDHAINVDDAYGF
                     TVKPHEESVFVFQVDNPKAAEKALKDAGYQLLTDKELYLL"
     gene            26022..26384
                     /locus_tag="PLV81_00755"
     CDS             26022..26384
                     /locus_tag="PLV81_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08662.1"
                     /translation="MKQYISILFISFAFILLSCSQQISIQGKPDVLIKKYLKDDSTYV
                     IMCKGYPKPNTTGIQATETAKEAALLNAQYIARGIFSEEVDVVKDGIVDGFSINPDYV
                     VITYSIRHPNIKRYIRDE"
     gene            complement(26765..26911)
                     /locus_tag="PLV81_00760"
     CDS             complement(26765..26911)
                     /locus_tag="PLV81_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08663.1"
                     /translation="MKKFASLILIAVFAVAVAFVGCKKEEAPAPATDQPVQEQPAEEA
                     PAQK"
     gene            complement(27002..27397)
                     /gene="queD"
                     /locus_tag="PLV81_00765"
     CDS             complement(27002..27397)
                     /gene="queD"
                     /locus_tag="PLV81_00765"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HOM08664.1"
                     /translation="MYILTIEDYISAAHQLKGYKGKCENIHGHNWKVEVSVYGHKLND
                     IGILIDFHDLKDMLKTVLQEFDHKNLNDIQEFAHQNPSSELLSKIIYQKIEKMLKQYS
                     QNHSTNINVLSVTVWESATCRSTYQKKFF"
     gene            complement(27420..28454)
                     /locus_tag="PLV81_00770"
     CDS             complement(27420..28454)
                     /locus_tag="PLV81_00770"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HOM08665.1"
                     /translation="MKIVSSKPTITRKELEGVLDCLIQDTLASGAVVKQFEQALGNLL
                     GMKYALATNSVTAAYHCAFMALGVTGGDEIIMPSFFHEAPLKACHLVGATPVLIDIAE
                     GAYHPSPELIQKAITQKTKAIVLGHMFGIPLGFDLGDSSVPVVLDISHAIGMDTADIF
                     LPSPSIAIASFSPSMMITTEMGAMVFTNNSRHYSYIRDVRNGVPHSHIPGFEYCMSDL
                     HAALGLNQLLKLKDFVRRRRDIARIYYEALNKTHHTTVYAYNDHCTYQAFPVLFDAQP
                     DIAEKFFKKNGIEIFHPVEVPLHQYCKLRNLDYPHSDRLSKKLFSLPIYPTLTKREIE
                     KVSRVLTMFA"
     gene            28616..29443
                     /locus_tag="PLV81_00775"
     CDS             28616..29443
                     /locus_tag="PLV81_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOM08666.1"
                     /translation="MRKFLLVQVAIVVVAAFAISCAPNNVMTKRDETYTFDQMSVQDE
                     TPVYSDMEPTQISSNSSDSSYYQVGIASWYGREFQGRLTASGEPFDMNKYTAAHRTLP
                     FGSVILITNLENGKKVKCTVNDRGPFKKNRVLDVSYKAAKDLDMLTTGEAKVGITVLS
                     YGNNTQPGTKSHDVGVFGQPVDNDNDNSNNAGMESKQFTGLALQTGAFYSKANALKLK
                     EKIEGLIDKPVTVVQEGYYYKVRITNMNENEVSRYIKVLKKNDIPSYKVTINESMND"
     gene            29876..31003
                     /locus_tag="PLV81_00780"
     CDS             29876..31003
                     /locus_tag="PLV81_00780"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HOM08667.1"
                     /translation="MNSKRIVHIDYLKAWAVIVMIEVHVVNCFLNPSFKTAWWFNIVN
                     FINGLVAPVFIFTAGFSFVFSVMKKREELISLSDLFFRRTGRILLIVLVGYLIHIPYF
                     SLKNIIIYASPAELKSFYSADVLQCIGSGLLVLLFLRMVIPSDVLYHSIITGIGLLIV
                     FITPYIWEIDFYQWFPLPIATYFNEMHGSLFPVFPWHGFLFLGAAYGVFQLHHNTNIH
                     ADKSLNTFAAISFIISIAGIISMHFLSQNPAYTIKTSAIFFITRYALVIVLLHCCQWY
                     LHIKNNQGGLFINIGKESLLVYWLHLQILYRHIFNGKSLERIVAQKFSFAECLTAFLL
                     LAALMFAVAIMWNTLKQHYPDISRKIFIGTILIGLIKFLSF"
     gene            complement(31006..31821)
                     /locus_tag="PLV81_00785"
     CDS             complement(31006..31821)
                     /locus_tag="PLV81_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM08668.1"
                     /translation="MTIQEQFIEALRLEEQGNVTEALQLYIQILQIDNSFRAALINLG
                     SLYYRMKQFNHALDCFLRALQLKEDYIVLFNIGSLYFRLGEYKKAIIILNKCSTSNPD
                     FYIALLVKGIAFSKINNYKAALGCFKEVIKKDPANKVALTAIILLYYEQKNYTDALRY
                     LQQYDRYHDSFRFREITSDIILQCARYNDEKLISHSLQKKGFRQFNEYISTIPHTIFN
                     DSKGTIDTKIQQLEQEIQKEPTPQAFISLSLCHLFVGNNQTALQYLVKATKAV"
     gene            complement(31873..32937)
                     /locus_tag="PLV81_00790"
     CDS             complement(31873..32937)
                     /locus_tag="PLV81_00790"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOM08669.1"
                     /translation="MDDNGLTFFDETEPTPEGIEGLEKKIYDLRNLLELGISLSSNLQ
                     FESLVESLLYSCIGQMFVEQVALLLQKDIDVNDFYIHMAKGYDSDFEENVILQETSPL
                     VGFLENNPYPIEYETLLELPDLADDLKLIAFLNPYIVVPLKSKNSLMGVLLLGEKITG
                     GGFSNSEKEFLHYVARFGAVAVENSRLYLMSTLDRMTRLYIHHYFEIRLFEEMKRSQR
                     YNAPLSLLMCDIDHFKRFNDTYGHLQGDSVLKEVAAIFLKDLRKMDIPCRYGGEEFAV
                     ILPQTRLDQAGIVAQRLRKIVEQHPFKGQDSTLHVTISIGVTQYDPARDTSIKEFVGR
                     SDKALYKAKEMGRNKVALAK"
     gene            33117..33551
                     /gene="dut"
                     /locus_tag="PLV81_00795"
     CDS             33117..33551
                     /gene="dut"
                     /locus_tag="PLV81_00795"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOM08670.1"
                     /translation="METIKVKLQPGAKLPDYQTIHSSGADLYALLDTPVTLQPLQRAL
                     IPTGISIELPPGYEAQIRPRSGLAINHGITLLNTPGTIDADYRGEIKVIVINLGDKPF
                     TIENNMRIAQMVIAHTLQVTFEVVTELTPTKRNDGGFGHTGL"
     gene            33548..35194
                     /locus_tag="PLV81_00800"
     CDS             33548..35194
                     /locus_tag="PLV81_00800"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOM08671.1"
                     /translation="MKEKKIRIIPIGGLHAIGGNMMAIEYDNEMIIIDCGITFPNGET
                     PGIDFMIPDFSFILENKTKVKGIIITHGHEDHIGAIPFLLQKINVPVYATKLTLGLVT
                     SRLAERPVNAPTFIEIEPRDVIHIGSFVIEFLRVNHSIIDGVGLAIKTPIGTIIHTGD
                     YKIDYSPIDGNVADLHRFAEYGQQGVLLLISDSTNATNPGYTPSEIVLKKKLLEIFAK
                     SKGRIFVASFASNIHRIQQVLEASQKYNRKVVISGTTMQKNIEIATELGYLKYKKDLI
                     IDAKDIGKYTHKKLVVLCTGSQGEPMSALSRMATGTHKHFRIGAGDTVIITASVIPGN
                     ERMVYSVINSLMQMGADVFYEQDEEIHVSGHASQEELKLMITLTQPKYFLPVHGEYRH
                     LKAHASLAESLNIKPQNIIVAQNGDIIELTPNRFEKKGNIPLSTVYVDGSYIGDITDD
                     IIAERKTLASDGVIVISIVIGDGLLLYPPVIIARGFIGIKNDRFMQVLQKELSNTIEK
                     QLLQNASPEHLTVAVKKLAKMIAHRFTNNTPLIETIIQEI"
     gene            35196..36026
                     /locus_tag="PLV81_00805"
     CDS             35196..36026
                     /locus_tag="PLV81_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOM08672.1"
                     /translation="MAYSIVIFCILGIIQGVTEFLPVSSSGHLALLEAIPQVHTIFGA
                     MDTGAKLFFNVALHLASLAAIIIFYYNDIIKLVTGTIAEITKKHYGTHCQFACNIIVA
                     SIPAAMVGLFLNDVVETSISSPALVAGLLIINGIMLIVSNKIPKKSRKCEEMGILQAL
                     LIGLFQAVAILPGISRSGSTIVGGLLIGLKPEEAGKFSFLMAIPVIAGAGLLELRKLG
                     TINAFEFGIPVVIAMAITFIAALASLKLLVAMLKSLHLHYFGYYTIIAGVVAIIILLV
                     "
     gene            36056..38491
                     /locus_tag="PLV81_00810"
     CDS             36056..38491
                     /locus_tag="PLV81_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOM08673.1"
                     /translation="MSNRVTEILCVILWGFAVIVFLSLATFHIADYDTLGTMPVQNIL
                     GPAGVILAQILRISFGIASYFVVAILLLTGWGLVKYKSLEPVLEHIFAITFAMVSASL
                     LVTIIQPETIAPFAGGVVGMALYHVFTSVMGLTGAWIATILLVLISLILLGIVSLSYF
                     FDSQRFTSLANVAALLYRKIVLFKHRDSIPLIDITSFNAEKKIPWITRTKITLYKKTP
                     VRKPKLIEYDNILTYKGDSSYYNKKDHTDDLQIINTHTTPTENSILDNSDSSSMNTAD
                     TESKEATSQVVAVTNAVIELPQVDSIQDEHEPTVITKEISDIDEHYDEDDEYDDATDN
                     EEDMVARNVFEEIPINQKYSIPVDFLKQYTVHDETGYKQEIAKNSELLVNTLRDFGIE
                     SKVVNVNRGPVITLYELQIAPGIKVNRIVGLADDIAMALAASRVRVVAPIPGKKAIGV
                     EIPNKVRETVTLKDIISSSEYTHQQGRLKVGLGKDIMGKPVVIDIKKLPHLLIAGATG
                     AGKSVCVHSIITSLVYNYDPNYVRFIMVDPKLVELQIYNGIPHLLTPVIIEPFMVPKA
                     LKWTMMEMERRYYLLSEYNTRDIDRYNQMVERNKNGEYLPYIVIIMDELADLMMVAAK
                     EIEGYITRIAQKARAVGIHLVLATQRPSVDIITGIIKANFPARIAFQVAQKTDSRTII
                     DQNGAEKLLGKGDMLYQSPMSSFPVRIQGAFITEDELTSIVSYLKKIGRPRYVDIEQS
                     LVEFEDDDEEGEDELFIEAVKIVETTKKASASYLQRRLSIGYNRAARIIERMEELGYI
                     GPQQGSKPREVYI"
     gene            38809..40470
                     /locus_tag="PLV81_00815"
     CDS             38809..40470
                     /locus_tag="PLV81_00815"
                     /inference="COORDINATES: protein motif:HMM:NF036774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HOM08674.1"
                     /translation="MKYTKQTTFIVIIIMAIALSVSAIPGKKVIGVTAFTNLSKDTTF
                     DWLCIGIADTISYKLRNVQEYILVDRVNVDKLIGELQLSQSGLIDENTVEAGKALGAN
                     ILVVGNFQKFGDQIRITAKIVDVQSHAVINQVQVTGSLNDIFNLQDSLALGIIEKNDT
                     AITTETKTKIVQNDTNNISAYEYFVRGQKFLLYQLDYSKAIEMYQKAISIDPNYAIAY
                     AGLGKAYSLRSWELREYHNTTDPTLIEKSFQSSKKALSLNPNLDEAHLSLARYYQEVD
                     QNKVPNKWTLCEDETRKALEINPNNGEAYLLLSKIYAYDIPKSEMYLKQAIEKNKFLV
                     DAHNNLGIIYLDRGDLDTAFAYIKNAIDIDENYVIGYMNLGVIYEKRGQYDKALEMYK
                     MAVSKYPNYILGLRNLGIGYRNLNRLDEALDAFFKALKLKKDDAQTLSEIGYVYLLKN
                     DYNKAINYFKQSLQYDPSYRYSLANLGWCLVQQGKYNDAIPYLEKAHNDNRDYAWAAG
                     YLGWLYENKIQDYNKALYWYGEALKRDPNNQDYRNSYNRLSGYYR"
     gene            40495..41493
                     /gene="amrS"
                     /locus_tag="PLV81_00820"
     CDS             40495..41493
                     /gene="amrS"
                     /locus_tag="PLV81_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010867880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOM08675.1"
                     /translation="MKEALYYTKHSEDAVQCVLCPHNCIIKEGKSGICNARQNKGGVL
                     YSLIYEKPCATHVDPIEKKPLYHFLPGSRSFSLGTVGCNLQCMHCQNWELSTSSPNDV
                     FCHTLTADDCVKLAIQYECQSISYTYNEPSINIEYVLETAIKAHDKGIKNVMVTNGFI
                     NEEPLRQLYKHIDAANVDLKAFDDEFYKKICKARLEPVLKTLTILKEMNVWIEVTNLV
                     IPTHNDDVTMIQKLVDWFVHNLGTDVPLHFTAFYPAHKLTNVPPTPLRVLKQAYDIAY
                     KAGIKFIYLGNVRERSVTRCPSCGRDLIVRSGFSVMRTNLHDGKCICGYSIPGVWR"
     gene            complement(41497..41781)
                     /locus_tag="PLV81_00825"
     CDS             complement(41497..41781)
                     /locus_tag="PLV81_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08676.1"
                     /translation="MEENKVFCANCMHCTVFRHYEGDNKTFVLRVRCNKKQWLKRSGE
                     EKVHKYFTVARRTMDECDYYVEAGDLYPYIKNLRKALPVKDELYQVEAHS"
     gene            41975..42346
                     /locus_tag="PLV81_00830"
     CDS             41975..42346
                     /locus_tag="PLV81_00830"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOM08677.1"
                     /translation="MQQSHIVVFGLPIYLDGITAQLKTVIDRIVCCMKPFLWTDVFGF
                     SRHSFSLEFPKDIIAVSTCGFPEYETFTPLISYFNALSKNLNSRLVTTTFIWGSIAIQ
                     MRIELLDLKLKLIESAGYECC"
     gene            complement(42535..44220)
                     /locus_tag="PLV81_00835"
     CDS             complement(42535..44220)
                     /locus_tag="PLV81_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOM08678.1"
                     /translation="MIKNLSEKTSKIKDIRLLILLVVLINIFFVTQIYSAEKPLIIVT
                     SEGFPPFSYIDKSGILRGMIIDFWNLWSAKTGIPVKIIHAPWKESINMIKEKRADIHS
                     GLFITKDRSMYMDFSKKFFTIKTSLFMRSNIDNPLNVILHDSSLKIGAQYDTGAYLFL
                     KEKFPWIHIKVYEDTARLIEAAANGEIDAFSEDEPSARYYLSNYHILGEFKIISILSE
                     EDIHAGVPKNNVNLLNVVNEGISLITEDDLLEMKRKWLVEESYNMVKDWRFYTAIMTL
                     IIIAAIIILVISFINRSLKHTIMIKTQELEKELLARRKTEQLLAQAQRAEVLGILSRG
                     MLHDFNNILMGITGPVSLLHKEIVNLAPDKASQLEKYFVLIKNSFDSAFDILNRLRSL
                     NQKKNNSFTQIDLNTAVDEIAQICKTAYGKKITITINMDNKKKFICGDSTSIMQILLN
                     VCINAIHAMTIMRDKEKSPHDNILSIDIIEKYDENKGRMFGVIITDTGVGMDEETIKN
                     LGKPFFTTKDANTGTGLGLAMVFHLIEEMNGYINIESTPGKGSRFEIYLPALN"
     gene            44605..45282
                     /gene="pncA"
                     /locus_tag="PLV81_00840"
     CDS             44605..45282
                     /gene="pncA"
                     /locus_tag="PLV81_00840"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HOM08679.1"
                     /translation="MTQLHTINNYGTATITRHDALLVIDMQYDFIPGGSLPVAEGDTI
                     VKPIAHLMEQFFIKGATVVCTQDWHPSGHRSFASTHGKHPYDPINEPGIGPVLWPDHC
                     VIGTKGASLHDKLPAHFCHAIVRKGYNINIDSYSAFLENDKKTATGLSGYFSNRGVKR
                     IFVCGLAFDYCVHFSAVDGASAGFEAVVITDCSKAVNSPATSVDDAIANMKAHNVSFC
                     SSADLSF"
     gene            complement(45274..46536)
                     /locus_tag="PLV81_00845"
     CDS             complement(45274..46536)
                     /locus_tag="PLV81_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HOM08680.1"
                     /translation="MDKRDTISYVQGKGGEVVLTPEEIKTLDTINQKIASGQSLIDII
                     EYLFKETQHIMPCDRIGIAFSDDGKRLTLYHVVASYSPLYLDKGYTADLKGSSLETIF
                     SNETPRIINDLEEYYKNNPSSESTRLLLKEGVMSSMTCPLYVEGRIVGVLFRSSRKKN
                     MYGLREIALHQAIAERLSQAVEKAYRIEQLTQAINAYMEMLSFVTHELKSPLSSIVTL
                     GTTLKQGYFGEIPEHCTNTIDRILAQAEYLLTIVNEYLNLARLETGSMPVRKSTVTIA
                     HDIIEPALAIVQPQADAKHITVEKDYEDVQLKCDPDAMKIVMHNLLSNAIKYNSDNGI
                     VRITVKKEGTICKISVWNTGPGFPESEKHKLFKRFSRIETDELLARKGSGIGLFVSWN
                     IVYLHSGRIYANSVLGQYAEFTVELPLE"
     gene            complement(47085..48257)
                     /locus_tag="PLV81_00850"
     CDS             complement(47085..48257)
                     /locus_tag="PLV81_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020415007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOM08681.1"
                     /translation="MNFSLTQEQQIIQETAAKFAKQELEPVASTLDTTKDRNILKSNL
                     KKLAALGFNGIAVDPRYGGTGAGSVAFSLVMTELGKACAATAVTTSVTNMVAEVIQSV
                     GTEDQKQRYIPPLCNGTFYAGSFGLTEAEAGSDPAAMKTTATDGGDWWILNGKKLFIT
                     SAEYAGVFVVWAVTNKHAAKGRGISAFLVEPHFEGFIVGRDEHKMGQLGSSTNEIILD
                     NCKVPKENLLGTLNDGFRIAVRELGGGRIGIGSMALGIGLTAMDYATSYAKERIQFDK
                     PIIHNQAIQWMIADNYTRLEAARLLLLQAAYKKEKGLPFMKEASMAKLFATEVANKAC
                     YDAMQILGGYGYTREYPVERLYRDVRVTTIYEGTSEIQRLIIAKHYMDTAPVPSNV"
     gene            complement(48272..49132)
                     /locus_tag="PLV81_00855"
     CDS             complement(48272..49132)
                     /locus_tag="PLV81_00855"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HOM08682.1"
                     /translation="MKPDTINTIGVVGAGQMGSGIACVAAVSGFTVILYDLTEELALK
                     GVTYINGLLSKDVAKGKITQDEKQRIMERVRATGEFHALADADFVVEAATENQDIKLK
                     IFKDLDTICRPQVICATNTSSIPIGRIAAATKRPDKVIGMHFMNPVPVMKLVEIIRAL
                     PTSQETFDVTWELSLKLGKTPAEAHDYPGFISNRILMPMINEAIYCLYEGVGTRESID
                     TVMKLGMNHPMGPLALADLIGLDTCLAIMETLYDGFKDSKYRPCPLLRRYVESGWLGR
                     KTGKGFYDYT"
     gene            complement(49156..50325)
                     /locus_tag="PLV81_00860"
     CDS             complement(49156..50325)
                     /locus_tag="PLV81_00860"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOM08683.1"
                     /translation="MKAVIVSTVRTALGNFNGSLSSIPATQLGAIVIEEAIKRAGISK
                     EDVNEVIMGQVLPCGYGQNPARQAAIKAHLPWEVECLTINKVCGSGLKAVMLAAQAIQ
                     CGDADVVIAGGMENMSMAPYYLEKARFGYRMGDGQLKDHMVHDGLWDVVNDFHMGISN
                     ELCCEKYNVSREEQDRYAALSYERALHAIQTGKFKEEIVPVTIAQKQKEIIFDTDECP
                     KPTPYEILATMKPAFKKDGVTTAGNASIISDGAAALVVMSQKKAKELGCRIMATIGAQ
                     ASAGIDMKYVLMAPILAIPKCLKKEGISLQDVDLYEINEAFSGSTVGVLKELGLDSLK
                     VNVNGGSVALGHPIGASGARVLVTLLHEMIRQNKRIGLASLCLGGGEAVALIVKR"
     gene            50580..51050
                     /locus_tag="PLV81_00865"
     CDS             50580..51050
                     /locus_tag="PLV81_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08684.1"
                     /translation="MNIETTTCISHEHLELLTLHANNHSMSLRTFISCLISFAAHCDT
                     VQVQYFKQVKYRNRNSGNWKRFHLVLYNDEYEFFMDVKKLWKMSLARIIEYCLDNVLY
                     DFLKFLTKAEEDEDYYTDNYRYGGYAFETGTKENIFYITLYWGPHPEILQKAMT"
     gene            51313..52050
                     /locus_tag="PLV81_00870"
     CDS             51313..52050
                     /locus_tag="PLV81_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08685.1"
                     /translation="MMAKKYKAVTCIVLVHIVFILISCTSVEFVKTGEEYPALNETDE
                     VQVLLTIDKNQYDEIGIADIGGVSLPVRIEKAKQIARSKGGNVIVPVTMCTEEAQKRM
                     RSGYIIQSFYILKTKTEAIAPKEAIESKQITPPVEEEFIPEKQAATFQQLLQHYPSLQ
                     GQPFTNTMVPLRFYKIPPSLLVFGLSDYKLCLMQFDTDNTVLVFIPKEKITVIKNSID
                     TRSTIAIDFTPLGVYKGKYPVLKMLSE"
     gene            52070..>54551
                     /locus_tag="PLV81_00875"
     CDS             52070..>54551
                     /locus_tag="PLV81_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08686.1"
                     /translation="MVQKVSPQSSLCRFTLNDEEKLSDERVKEEIRKAFQELRAFPFV
                     VNSGIIDSLKKGADPSSLKDLFVAIRNDIFAAITDSPQAKEKIVSDTRLQERILNAII
                     TIHSAIAKGDFTSVINRRFVLIKERPAWPTLYYISPQSAVLSHVGQGPQWTEIPTIYL
                     GLNIFNVLALDKDQKFDKAFYWLLRVEERAIETGYSHIEVYPPHVSRALNELLDEVIR
                     IATISEVEFKEIPARKKIRAFTIRNRESFLKMLNARRDDGNFDFEKNLKAIRSLERLA
                     RRYKKGYDFNSLREIVRLLVAASGHDIHEIRNNANILLERIFAPKEFDAPLANTFVNC
                     AAGSIHTFEFSIPEEGNYFVRIFYNAGTNPYPLEQDIAYSDIDLAFDETKRVYSAHYT
                     FNELGHYDFLVYRKRKKRSQWVTYEGCSGRINVIPDVRGEIVLEIFPDIHGHTRIYWM
                     DEEHPGLVYNEHGEVIRLGRFSDITAHLEDLKERYHITALYLLGVQKRGTNREDWAPE
                     ATSPSPFSPVSLVEIEDFLGGENEFRELVSKAHAMDIKIIIDVVPHLNRRSTELPDEY
                     AVKCYDDSGNLVIRASTDGRYGSWNDGKLLNYRKLEVWEWLINSVVTLIDKYDIDGIR
                     FDSAHAVPIMMKKNNYPFIWGQYRSLESLVEGEIIVNDREDGHFITTGYFDSACRDQI
                     AIPFHHLLMCRIAQKLKEKNKTFFVHLAECYWGHERYLTRSGIIPYNSALFKICEGII
                     HGTTDVREVYHFYDNYLPYALPPGTELLGILGNHDERRALNTFGHRGLRAAIGLTIFM
                     NNIIMDYEGSAEGESWKVFLDNIYVNWNQ"
     assembly_gap    54552..54651
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <54652..57066
                     /locus_tag="PLV81_00880"
     CDS             <54652..57066
                     /locus_tag="PLV81_00880"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOM08687.1"
                     /translation="EGESWKVFLDNIYVNWNQFEYAAHRSLESFYRQWYRFHRINKGK
                     GYLIWANNTQVAASIKFTEHTIWIGIFNFADSSQNVALQFDNPRLPIADDTYFKVVDP
                     VYSPITKHYSYFTGKELKASKIYSVVSYTDRIKLLKLEPVSDVAPLYSEFLRDSLFRL
                     YSISNPENFKSNFMFLETIAHSSTFEAFLTFLKNHIIAQFYPQYKNFIEIGFKRILFY
                     MFKFGFKSGNDIVQLIDDLAEHDDTNISDLGKSIKFHNRPGPIIFVSAEAEPFSKSGG
                     LANVVYELPRELVSLGEEVIVIIPKYRHGDEKAMEKMNNALKKYNVQYTGKNVRFMIE
                     HATYEAGVHYAQVDGIHYYLLDHHEFFDGLYWGYTGQEKLRRRIAFARATAELITTFG
                     LYPLFVITNDAYTGIFNGIVRSDHVYYDNPNFKRTSFFHIIHNGGWQYFDSYHRYEDG
                     KDLFSLFNLPHWRYTDFSDPNDYNKINCMATGIRFADRVITVSPSYAKQIEKACDGLE
                     KILHNVIGISNALGVDFKNRILMRFHNSGFIDEYYPRMVDALTSSTATYTKIKQRYPE
                     ILEGIRNCEKITDPLRREYVTHIRNKLCLQCKLGFEINPDIMLFSMIHRIAEQKGFQL
                     LLDASYGIFTTLGFQGVIGGAVAWGDQRGSELATGLLHLQNYYPKNVNVNLGFQDISI
                     PLLCSDVFLMPSLHEPGGISQLEALACGCLVVARATGGLRDTVIPIRMKDSSVEGNGF
                     LFSDFTPSSFYDAMQRCAEFLRTSDDTTKALARKNAMQSVYYWDKPAKEYIDVMYSHK
                     EIVRVV"
     gene            57181..57753
                     /gene="lptE"
                     /locus_tag="PLV81_00885"
     CDS             57181..57753
                     /gene="lptE"
                     /locus_tag="PLV81_00885"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HOM08688.1"
                     /translation="MKKKYIIAVISLWILSCSSNDKAELGNIVGSINGQPVIPYAANK
                     LYISPFTNQTGILGYEEKIKQRIMEKIITGGRLAIAPVQADSDVILTGTIQSFQVQPI
                     VIDSAGIIKKKRLLLITVISLINAQTGNYIFRSITVQAFKEFSDIEAPVTPITIAQDE
                     VEQVLASRIALQVETGWYTSLKTGVEKGRK"
     gene            57755..58762
                     /locus_tag="PLV81_00890"
     CDS             57755..58762
                     /locus_tag="PLV81_00890"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08689.1"
                     /translation="MASKALMANDLKSVIQKELSHIYCFCGNDENQKEEAVTLIASQL
                     WKNSKPQDYKITTYFAEDSLEQALAAIASVSLFDDTVLYIIKHCEQVSGSKKIHTMVL
                     DCINNLPNSVFVIFDTQENKLPSLLPSQIHPHIPLIQFYRPFEENCKSYILKKCKDLS
                     LTISHEATERLVELTASNIKEIDDILTTISFSGHTDISKEFLEGFVINEREAIEFEFV
                     DCFFLKDKRAWDLLSEAIERNISQILLVSLLFNHAQKLEQFHIQTAKGVQFESLMKEL
                     KIYGKRQKVFAKQASSYTLDNVRKLIAMLYTYDIQLKSYHSGGFPQENPLLDLLLQIS
                     M"
     gene            58776..61499
                     /locus_tag="PLV81_00895"
     CDS             58776..61499
                     /locus_tag="PLV81_00895"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HOM08690.1"
                     /translation="MDLLQTIIENKEKFIAFNNEKFKRFQQLIPNPAMRRIVNTIPFL
                     LCINNKKMPGYVEGDVPLGIKNFKLDEDTKRYLHGRYPGITFHDFERGDFIKMFAVMG
                     SVGTVAYNKKSDLDYWACIHKNTVSKEAFDNFRKKVTLVQEWASKELEVPVHIFINDI
                     ESVKNNIFAEDEEEAFGTTVGAVLKDEFFRSSIIIAGKIPFWWVVPSFIKDDEYEALY
                     NQLPDEMKRDDFIDLGNLYEISKEDFLGAALFQIIKSLGNPFKSILKIGVLEKYLFGK
                     SDSPLLSQKVKMHVLLSRLTNSILDAYLLMFDEVYDYYSQTMNDPAILQILKQNLYLK
                     IDPQLSKYVGIKDKKNIPYKVAVMFKYVHDWNWNIKVIQDMDNFDNWDFQRVMHFWDA
                     VKKFMLISYQKISSQFSTLNLKQQISETDFTLLSRKIKSHFSVEENKIDKLITFKDTP
                     AEPILYIEPVNEGIREVKWRLFKRNRSEKNVFTTTTLRTESDLVKLLVWLTVNNVYDH
                     SVTRVNIQSGYVRINQNLVVELLNQLSQFFSEKGKIKTKYYLKPAFNMLQCIILNFNN
                     DAAEHLDSLYHVYSTSWGEQYIKQYFNIIDIAHILQNILKDGLELKLPFDDYCLINTP
                     GTHKRFCKNFIQLFRESYNAIVHSPTPHSRFIGELEGLYFTALRNNTIEVQAFDHIVK
                     VLTTLTLKPGIEAHYTFYGDSYHLQVLQALYNKRLKKGITIGYLEDSDKLHVYVINEK
                     GNIFTFFAPKNIKSVFLYHIYGFCTNVLKRLPSSVRESESSIICYGAQADRRGGISLI
                     DETGKIHGDYLLNFTKSKALRVQIARHMGNEIFYNFIFPDNVTSGFMTIKELYSVKRK
                     IIELKNMGMPVYPYIGELVFNDLNQKEILLGSSIYFVEKYKIELMLQRTQA"
     gene            complement(61506..62039)
                     /locus_tag="PLV81_00900"
     CDS             complement(61506..62039)
                     /locus_tag="PLV81_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08691.1"
                     /translation="MKVIHVLKRIEMIDQDIRELRKLEKSLQRNKSFTQPIFITIEKQ
                     INILLGERIKLLELKIANPPANLQPEEEEVSEALIAEPKKKTSKKKEPKENKIKDTVV
                     ELEEQEFSMLTQDDIDRKIAKLQQDDEDKAKKDEDDTAVKLLDIALEKGTINKQEVET
                     QKKKIRFFKDNFPGGEY"
     gene            complement(62211..62771)
                     /locus_tag="PLV81_00905"
     CDS             complement(62211..62771)
                     /locus_tag="PLV81_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOM08692.1"
                     /translation="MFIQHTVKDSVAIVTVEGYIDHTNAVQFEEYIATLQKEFNAIII
                     DCSKLNYISSTGFGALMYAQRVVESGGGVVVLCHCNKEIVTLIDIIALPVKRYNSLDE
                     AMEAVQDTEVKIIEKPVPPKESPKKETYTETKPVARTSMVFEHPLVIECPDCRTMVRI
                     SSPGKFKCPDCGLQFTVDDDQTVYFN"
     gene            complement(62778..63587)
                     /locus_tag="PLV81_00910"
     CDS             complement(62778..63587)
                     /locus_tag="PLV81_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08693.1"
                     /translation="MQNDKQVQFPYAYVIVLFLIIAILASSGALCYKYRQLVSQIDEF
                     TIQAKNVSKTLITTYADAAELAYATRSTDQLLSLIQESKNIKGIKEVFFVLDNGTIIA
                     HSDTEKAKELKGNIANDEFYYNIDMILYPHTKQSRELFIFNYFVPDLPLPFTTIIRKY
                     IQQFLYKDINTNSWLVTKAVYAKGKSIGTINCIIVKTPIYDMMNSYYKELLYMLIIIC
                     GAALVFSLCTGIVLFIKQKRVLQEVKEFSAHIHDKPGQIRSIIKEAIPVKE"
     gene            63755..64411
                     /locus_tag="PLV81_00915"
     CDS             63755..64411
                     /locus_tag="PLV81_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08694.1"
                     /translation="MAIKEEILEEWNWEQGVPTGKPVARSIAHKQGIAHEGVHLWVVR
                     TQPQKEILFQMRASHKDMYPDYLDITVGGHVPFGLTSNKIQKEAYEEIGITFDEKQLI
                     DLGYYRYEEINDLLFHREFQRVYLLLSNNPLSSYSFNDNEVSSIYAVPLDYCKNLFIK
                     DYSVTVESFNGTDTIVTTISKKDFHPLLFAPSMERYMEILFQAIDELFTKGTVSVSMS
                     "
     gene            64427..65446
                     /gene="mnmH"
                     /locus_tag="PLV81_00920"
     CDS             64427..65446
                     /gene="mnmH"
                     /locus_tag="PLV81_00920"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HOM08695.1"
                     /translation="MSVPTISYKETLVWENPVYVDVRAPVEFVKDHIPHAINYPLFND
                     VERAYIGTLYHTQGKEKALLEGVSIASLKIRDMLSFFSTLKEHQVIIYCYRGGMRSES
                     VVSLCNSIGFSFYKLYGGYKEYRHYVRNYLTEYTFTIPVFTLYGFAGSGKTKVLKNIP
                     YSIDLEHCAGNRSSLFGSLGMPNHTQKYFESQLVQELRTIESWRYCIFEGESRKIGNC
                     HIPHCVLNSLQNGKAIWLETPLEKRIQHIYDEYMPFIREETILPILDKLQPTMGKAKT
                     AYCKQLFNQGNIHELIEFLLLHHYDLEYRHSTKNISFMAHILFKDPVTTASEIDSLIK
                     DMLAV"
     gene            65565..66137
                     /locus_tag="PLV81_00925"
     CDS             65565..66137
                     /locus_tag="PLV81_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08696.1"
                     /translation="MKKLSVLILAMLLGFVACGGGVQYQDASKAEGSREWGPKEIKMT
                     VNKMVESMYKFLKEEWKKPAFIEVKQIRNKTSEHIDTQMLADEITTNLIKRRIKFIDQ
                     SLTADAIAEMEKGMTGMVDPESAIPVGELKSPNMYLTGEVRENVRTVGSKSVQYLVVT
                     LKLINLKTQVVEWQDQQEFLKSASKKKITF"
     gene            66153..67973
                     /locus_tag="PLV81_00930"
     CDS             66153..67973
                     /locus_tag="PLV81_00930"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOM08697.1"
                     /translation="MIQKIKSLFVVMLLIGVAACASYDYNKQIAEVEKMFYEGQYKEA
                     ARKLLPMVNKKSKDQLLYMMECGLMLQVAGDYENSTKVLTEAAKIGDQITVSLSKQAA
                     SLLLNETTTNYNGEDFERVLIHMYLGINYLMLSKPDEARVEFKKVNDMLRDINVTTGK
                     SYKQNLMAKYLTAIAFELSADVDNDMNDREFAYIEYKQIYELNPALSMVYRDLQRLAK
                     QLGDTEDYNSWIGKFGKQDAIPNNAGELIVIVQNGKGAIKQSRGPLLSDQSMKATIQI
                     TLNGMSLAEGVTVAGVLLALHNAENPIPKFVKRGNNINYLIINANGKDVGRSIMLEDV
                     ASTAVKTLEDDYGRIYAKVAAGIAVKAAAAVAAGYAAKKLAEESKKMGGYAGLIGTVV
                     GTGVGVGLLSQIKPDLRCWHTLPENFQLSRIFLPPGNYTITFKFVDYSGNIVSTQNET
                     IEIKSGKKVFLNKRILPTKGVSRFSIDIESGTGKGYLGAKIKSITEEKARELGLSFSS
                     GVYVNDIIPESPAAQSGIKINDVIIAADGKSIQKPQDLVQIVRNSQSGKTLQLTVIRN
                     KSQMTIPVILGSLPEKAYNATIPSNPGHNSSEAPTTNSPE"
     gene            68046..69902
                     /locus_tag="PLV81_00935"
     CDS             68046..69902
                     /locus_tag="PLV81_00935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM08698.1"
                     /translation="MQNNHNSMVPINDMTIHSATEMVLMFQLPACVISVSGEVIASND
                     AFSDLVNVKNLSLDVTHPFYPEYRKQLATSYLKALKGLSRQCFAVFRNKDNQRVSTEI
                     YIFPVFQDTAIVALFVLLQIVDERILSFDKSTDTIISQESKNYESLLYEYSPFPILQI
                     DQSGNIVRASPSVQELIGYEIEDLKKNRNLLYRAMNHYDFNRMRLTLSQIFEGSIGYK
                     RIGEIRFITKDKDERWVNATCYPVHIQGTPPIVEIILEDITRIKRMEYQLQQLSRFHI
                     IGDLTKGFLHSFNNIINIMLNKAQWLLQITEKPSFQEGLKLIIKSAEDGIQQIRRIEE
                     FIGRDKLEEKCDINVVEAIEDAIEFAKIQFKVEEAQKRRSVAIERRYFSLVSMYGDPH
                     IFRDLLLATIFKVASVIATKGVVEVTLKKNSTLTIAIKAKKELFETKDEFAFISTIDL
                     SKLAEQNHAKLMEEESLQDYSITIILPEDTVSMLPHKEQQIPQIKLRDMDICIVEDEP
                     ALQEIMYEVFDSLGNRVSVFNNADQAYESFKVNKFDIVIADYGLSGTTGLELLTKIKE
                     QKEETITVLLTGWILNNIKAYKNVIDIYMHKPFKLDVLINEISNEMSKRHTF"
     gene            complement(69906..70202)
                     /locus_tag="PLV81_00940"
     CDS             complement(69906..70202)
                     /locus_tag="PLV81_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HOM08699.1"
                     /translation="MAEVLTRPTVVDFLDTAMMDSELGLAMEELKVGPKSPMVGKSLI
                     ESNLRKDYGVIIVAIKKAQGKMIFNPQSSEVIESGDIIVALGKKDMMEKMSTIL"
     gene            complement(70187..70894)
                     /locus_tag="PLV81_00945"
     CDS             complement(70187..70894)
                     /locus_tag="PLV81_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HOM08700.1"
                     /translation="MEFKKLRVALILLLGTIGFGTAGYYIFEDMKPFDAFYMTMITIS
                     TVGFQEVKPLTIYGRIITITIILSGVSISAYAIGTLLRMIIEGELRKEFGRKKLERQI
                     QSLQNHYIICGYGRIGKLICKELYDRKVPFVVIENNPAAIEELEKLSYLFVAMDATSE
                     EALLKAGIMKAKGLVTAVMSDADNVYIVLTARGLRNDIFILSRGSDEKSEVKLLRAGA
                     SRVILPYKIGGNVWLRF"
     gene            71113..72084
                     /locus_tag="PLV81_00950"
     CDS             71113..72084
                     /locus_tag="PLV81_00950"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HOM08701.1"
                     /translation="MAAFQHSTGTFIGKGGIEIFFQQWEVANPKAVLVIIHGLGEHSG
                     RYGNLIDALQNKGIAIFGLDHRGFGRSGGKRGHVDSFMDYIYDMKIFVNMIRDKHPNK
                     PVIMLGHSMGGVLALKYALTHAEDLDALILSSPGLVPAIKVPAWKKNLAIFLSSRIPS
                     LTMPSGLDATTISRDKEVVKQYLDDPLVHDKVTPRFYVEMMNTIDECINRCGEIKLPL
                     LLFHGTADALVLDEASKIVYQKASSKDKTLKLFEGLYHETMNELEPDRKKVLKVVADW
                     IVAHAKKTTTTKPKAASKPVKKAAPKKAKPKKVIKSKPKTSKKAKKK"
     gene            complement(72206..73054)
                     /locus_tag="PLV81_00955"
     CDS             complement(72206..73054)
                     /locus_tag="PLV81_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOM08702.1"
                     /translation="MIINHNISAIFAHRTLKFHDWAITKDMEKLSSGMRINKAGDDAS
                     GLAVSEKMRSQIQGLRQAERNTEDGMSMIQTAEGYLQEVHDILQRIRVLAVQSANGIY
                     TPEDRQQIQVEVSELVDEIDRIASQAEFNKMKLLTGAFARLHPSASMWFHMGPNMHQR
                     ERVFIETMTTAALGLRNPVTLTFISISTPGKANAVIGLADEALRQISKQRADLGAYYN
                     RLEHAAKGLMNAYENIQASESRIRDTDMAEQMAEFTKNQILTQAATAMLAQANTKSQT
                     VLQLLK"
     gene            73438..74901
                     /locus_tag="PLV81_00960"
     CDS             73438..74901
                     /locus_tag="PLV81_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOM08703.1"
                     /translation="MNKHTDKIFSLIESTPFLSNIIKKGGIQLKWVVTVSMLMVIVIA
                     VISFLLISISETALIKANDKLCQTIAYNISSSESFLTAEKKPLKRSMILQDIVKGLSK
                     TNIEGFVYAAIYDTQGIIAEKKYTYAAHSNAKLHGKYYSKDLLKQFESIHTTVKEKIE
                     LTIDSKPTVCYRYRVPFEFFKAKIGIIEIVFTEDAILGPVHRARLVMLIVAVILLSLS
                     IYAAMKIARQMVEPIVQLTSGVVRVKNGDLTTRLTITTHDEIGILTEEFNEMINHLRE
                     KLKMEKFVSESTISMIREKRDDELDLGGTRKNMAFLFSDIRGFTAMSEKLPPEEVVKI
                     LNEYLDLQASIIKKHGGDIDKFVGDEIMAVFEDKKKADSAVEAAVDIIESIENLNKQR
                     AKEGKITVTVGIGIHMGDIVHGRMGSKERMDFTSIGDAVNLAARLCSHADSQTILVSQ
                     EIIANLTKRKFKGKKLEPIKVKGKEHPIPIFQITGKA"
     gene            complement(74902..75291)
                     /locus_tag="PLV81_00965"
     CDS             complement(74902..75291)
                     /locus_tag="PLV81_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08704.1"
                     /translation="MKRLEILNNYLATHTVPELVAKKLDANSFLTNNFAYHALRIGNS
                     IGDNLDISIEIIILDEIAKKYNLILNTTEHAELHTQGITEADLDSLVQAAILFENIKN
                     NKKQYKEILRKISYFIRKEFYPVIHQD"
     gene            75662..75943
                     /locus_tag="PLV81_00970"
     CDS             75662..75943
                     /locus_tag="PLV81_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08705.1"
                     /translation="MPRKEYISGTFCIDIKCTHHKALEQYSGDEYFKQKALLCADCYA
                     WKFFTWLRDHKYRIIQTLPEISARELAARIKGIDPVRVEDLTEDEILCL"
     gene            76003..76992
                     /locus_tag="PLV81_00975"
     CDS             76003..76992
                     /locus_tag="PLV81_00975"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOM08706.1"
                     /translation="MVRNIGIVIVSIIIIAIVLDAIVSILSRPQAGRKACQDAGIPYK
                     TVIAHRGASYWAPEETYFSYSIAKNVGADYLEMDVQRTKNDVIIVFHDDTLNRITNVA
                     EVFPGRENDPINSFTIAELKRLDVGKWFNTSFPERSRHSYSGTQILTLDEVIDIATSD
                     KGLYPLYIETKSPKNYPGIEEQLVHILQTRGYIENENKINAPYAPVIFQSFEIDSLLK
                     LQKLAPQVPRIYLIDEDMVNQKGWDTLIAEAKQAQCAGIGPSGYLGWPWITGKAHRNG
                     LLVHIYTVNKKWQYRLLDWFGADGFFTDRCELLMEYYVKPHKKPFKEILANID"
     gene            77277..78494
                     /locus_tag="PLV81_00980"
     CDS             77277..78494
                     /locus_tag="PLV81_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOM08707.1"
                     /translation="MSEWSIAGKGILTPDKFVKKASILVSDGIIDKIDGRSSFSIEVD
                     GIIVPGLINSHDHLLGTYHPKVGNGPYENWLPWDNDLKSSPIYEERQQIENRDLYLLG
                     GYRNLLSGVTTVSDHIPHFVNEPFIDILPVKALKNYALAHAVVSFALAWGDGITLEYQ
                     KAAKEHIPFITHSGEGFDDETRHDIDTLERLGALGEYTVLIHGIAFNEQDIKRIKKAG
                     ASVVWCGDSNMYMFNRTANVKLMLEHGVNVCIGTDSPMSGGENLLYEIKFDRKLYKKL
                     FGQELSDETIVKMITVNPSYAFKLHDHGAIKPGNVADFIVISRMEDNPYSAVVNAQLE
                     DIALVVINGKPMYGDSSYKKLFDHNKVKYQEIILRNRHKLVVGDLLGLLRRISKAVGY
                     KKEFPFIPVEFDI"
     gene            78512..79663
                     /locus_tag="PLV81_00985"
     CDS             78512..79663
                     /locus_tag="PLV81_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM08708.1"
                     /translation="MSNGSPTQGITTRTYKPGSIVYFENDKSENIYILKSGLIILTSQ
                     KLETGEEVKEEVRPGEFFGVKSALGRYPREETAQVIKESVVLVLTLADFERLILKNVN
                     VVLKMLRIFSNQLRRIGRAVREVLGETSTINPEEELFKIGEYYYRAGRFNQARYAYNK
                     YLDYYPNAKYSDLATKRIREIDSGQAESNIAPQPVKRKEVEPSTEELKDFTLDEDIDS
                     MPAKESVVDEMDDFLGENDSASSDFDIADESPKHEDISSMFYDAMSLYSQSEYESALQ
                     IYKKILNQKSLKNDAENKIYQKAHFEIGRTFFKMGQYNEAIEAFSLLMKKFPQSELNV
                     NALYHLAMIYEVKKQFDKAKSYYQKVISLAAKEEIGKQASRRLKNLESR"
     gene            79673..80317
                     /locus_tag="PLV81_00990"
     CDS             79673..80317
                     /locus_tag="PLV81_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOM08709.1"
                     /translation="MLLDEKLFDKFGVEYEPNDIIFCEFEPGNDFYFIQSGRVEIVKI
                     INNREKTLDILGPGDVFGEMAILEEEPRSASAIAIEHVKLLRFRRENFDALLQGNPQL
                     AYKLLVIFSKRIYDAKRRLMILLLDDPQLKVMDVMNMLAEQDPHLDVQEPVTLRVTVQ
                     EIANWAGMQINDVQKILTHLNKLGKIELFNDRVVVRNIRDFQRLVNSRRKSMYA"
     gene            80318..80391
                     /locus_tag="PLV81_00995"
     tRNA            80318..80391
                     /locus_tag="PLV81_00995"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:80352..80354,aa:Arg,seq:cct)
     gene            80453..81523
                     /gene="prfA"
                     /locus_tag="PLV81_01000"
     CDS             80453..81523
                     /gene="prfA"
                     /locus_tag="PLV81_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOM08710.1"
                     /translation="MEERLQQIINRYNEIEHAMADPKTVSDQSLFKSLTKEHARLTPV
                     VQKYYEYIRIKKEIDDTKTALKSEKDQEMISLLEEEIPKLESKLHEIEEQITQMLLPQ
                     DPHEGKDIIIEIRAGTGGDEASLFVADLYRMYIRYADLKGWKVEHIDANQTELGGFKE
                     VIFSISGAEAYSCLKFESGVHRVQRIPVTESGGRIHTSAVTVAVLPEVEESEVDINPD
                     DIRIDVYRSSGHGGQSVNTTDSAVRITHIPTGIVVTCQDEKSQHKNRAKALRVLRARL
                     FEKMERERMEKEIALRRSQIGSGDRSERIRTYNFPQSRVTDHRINLTIYDLESVLDGE
                     LDKIIIPLRENEMEKLLKASHV"
     gene            81516..82382
                     /gene="prmC"
                     /locus_tag="PLV81_01005"
     CDS             81516..82382
                     /gene="prmC"
                     /locus_tag="PLV81_01005"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOM08711.1"
                     /translation="MSDVFYNNSIIQIIAFASKRLESISATPVLDAEILMAHALGIDR
                     HKLVLYHNTLVDKKTLTKFKRLLSRRLKSEPIAYIVGYKEFYSLPLKVNRHVLIPRPE
                     TELLVDCAIYHARQRATILDIGTGSGAIAIAIKYNRPDCSVWATDISKDALKVAKHNA
                     GTILGKHSIKFLHSNLFESIPTMTFDVIVSNPPYINPNIYTQLQKDIHYEPKEALLAP
                     DNGFGIIKSLIEKANNYLSDNGTIIIEIGEDLSSRIQNFAPLFNFSISILKDYSGLPR
                     IAIFKKIKNINI"
     gene            82515..83423
                     /locus_tag="PLV81_01010"
     CDS             82515..83423
                     /locus_tag="PLV81_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08712.1"
                     /translation="MINTREELFHDSNLPDFVESYRISVISKRDKWYSFSNITWNPHI
                     REIGIYSLINFSDFFVTGSQFIKDTVPKKSFGSQRSKYTIIQPLKESKLVYKDSNLSA
                     SISLLDYFPYYELLHNKKPSIESFENMISSYFTRCKAKVSVEVHTKSKIIKKNFDCIG
                     FKEHTWGLYPHTQINCDSRVVVHFRDQTIAFRYIEYNGSSYSYGCISRKNGNLALVLI
                     ELEYLSIKNAQLESSEFTYKDAQDDIDLIVSKPIKTQVIDVPKKAKKSHIHVVSFSDF
                     TVIGTNKKGYGIEEHYISYDRLKNTL"
     gene            83432..84751
                     /locus_tag="PLV81_01015"
     CDS             83432..84751
                     /locus_tag="PLV81_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08713.1"
                     /translation="MIITSEQLKRMGLFIILLIILLVYNLYSKLYYNWYGIDIIVRSY
                     SFLFSFLCIFNYTQIELKSYETVYRNRFPKFANLIIFLESRVIPFLLIYFISTLHILI
                     DNINNSSWPYTAYIGILDGRYTNIIFYSLILFGVLRYKIKPTIAIPLFIGGSIAFYFV
                     DKLIYSNIIAGTAIVFIKLLKLTVLTGVLVFEYFHVNIPQLLVIAIATGGILFSGSIG
                     TYIVLYSTVKHVPIKKEIQFKLLRYGIPFRINELKQYVLTKRQYKDYQLFIMYSSALD
                     LPVNFADEEWSNLLFSENIQMADEVASVLLKKSITIQFKSIIDYAYTISLKQNEQLQS
                     ASHLARLAARFADGNEKTIISMFEKGNISLKIWIMSVMGFHKKIIYLPALLPYCADTN
                     KTLATSAYSTLVQITGKNPSAEFNLPTNDPQVLKAFKDFYFQSGNTK"
     gene            complement(84714..85346)
                     /gene="nth"
                     /locus_tag="PLV81_01020"
     CDS             complement(84714..85346)
                     /gene="nth"
                     /locus_tag="PLV81_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOM08714.1"
                     /translation="MIDKATSKKIINRLKKQYQVSAPLHFNNLYQLCIAVVLSAQTTD
                     NQVNRVTPLLFKKYPDFYSLAKAHIDDVEEIIKSTGFYHNKAKHIVALAGTIVHTFNG
                     VVPETREELMSLPGVGRKSANVILAFGFGIPAIPVDTHVSRVASRIGYANSRKPIAIE
                     MAMMESIRKSDWIIAHLLFIYHGRTICHARKPACSRCPIILYCHFENKNL"
     gene            complement(85336..85866)
                     /locus_tag="PLV81_01025"
     CDS             complement(85336..85866)
                     /locus_tag="PLV81_01025"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM08715.1"
                     /translation="MKKCLLFIICLTGILLYYCSSVQYGKHKSLWRFNDPYINQTIIN
                     AWENVYKRRYEWAALDFTRLIQKNYIDYDILFGAGLSYYFMNESKKAMDYFTQAIEKN
                     PHHFEAYYYRAKLLLSQGAHAKAKQDLEAIIYMKYDEPLICGYYFTNDDIADIHVFKS
                     RQKEAQSLLIAIAHDR"
     gene            complement(85863..86462)
                     /locus_tag="PLV81_01030"
     CDS             complement(85863..86462)
                     /locus_tag="PLV81_01030"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HOM08716.1"
                     /translation="MEIVVATRNKGKIKEILSFGIPSIIFTSLEKYPEIGEIEETGST
                     FTDNALLKARHVCNSTGLPALADDSGLVVDALNGEPGVYSARFGNLKTDKERYMLLLQ
                     KMHTVPDDRRQARFVCVMALVFPDGREYIEEGVCEGVIAKEPAGENGFGYDPVFYIPE
                     LGKTMAQLPLDVKNAISHRARALQKVKILLEKIVGEKTI"
     gene            86631..87305
                     /locus_tag="PLV81_01035"
     CDS             86631..87305
                     /locus_tag="PLV81_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08717.1"
                     /translation="MEDQKQLNILRIGIANLPELEELVKSFRLMNQYSKRRRFVVSRE
                     DLKDIYGNIIVEKAHDINISVVKLLQRNFKPDTDFKIFSSDEGIAIVTNTESPNAKEF
                     SSQLISTIEGIGGGIYKPFIDTVPGFYELFKLFEKGLSPKLVVVGYIPVKQVAQEVSM
                     YNEIKKYDPYIRILDITHDELKPKSFLKGAVTFHFDNAHKVWQRFLTKLIQEYTKPYF
                     MEQITK"
     gene            87317..89359
                     /locus_tag="PLV81_01040"
     CDS             87317..89359
                     /locus_tag="PLV81_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM08718.1"
                     /translation="MKDKLLQFFAITLFTIVIAVTTSITISMYFVNAHSWVFYLSIVC
                     TMLLIVLFVYIRIIHKILFIDKIEKLHAIAENFKKGEFHHKVGQIPGDDPFAKIYTEL
                     LSASRNLENIIKSQKSEIEKLRELYTNMMFSMNAYIVVLDIHNTILFTNDLFLKKFQY
                     TPQEVSGKNITELFYFLTGTVLQAIEEIKKTHESIVLEKAHLISNNRITVIADIKLSY
                     MISNNEPQIIMVMDDVTSKTKRDYQISLITRLSEKIQKDDVLDHVLRTILTAVTSGTG
                     LGFNRAMLFLKDEKNNVLYGRMAVGPDTLEEAFAIWSSLSPQKDKEILSRGVNGDDAH
                     GQLLKEKVTSTSFDLSEETIFTEAFKTGETIHVYDAYHDPRVNDEIRSFIDVNEFVIV
                     PLIALNKTIGIVIADNKFNQMPITQDSVDLLTVFAYQAALSIESYNSLDLLKKEMEKI
                     KQRQEAIVESEKLAAIGRIASHIAHEIRNPLVTVGGYSRRILQNITQNPPDVQKIKKA
                     AQIIMTETERLEKILANVMDFTRPSPFLLKFNNINDIIRDTASLLSNLFQERRVELSL
                     QLYEVPLIKSDFNQMKQVMLNLLQNALDATPPYGVVEVKTYADDRYVYIDVSDTGTGI
                     DEENINIIFEPFYTTKTTGVGLGLAIVKKIVADHNGEIKVTNRERGAVFTISIPLP"
     gene            89487..89846
                     /locus_tag="PLV81_01045"
     CDS             89487..89846
                     /locus_tag="PLV81_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM08719.1"
                     /translation="MKKILIVEDEQHQRELYAMELQEEGYEVDQAANGKEAVDKVKVN
                     KYDCVVLDIRMPEMDGIEALGKILSRDKKVPIIIYTAYSNYKSNFMTWTADAYITKSS
                     NLKELKDKIKELVSARS"
     gene            89846..91066
                     /gene="glgC"
                     /locus_tag="PLV81_01050"
     CDS             89846..91066
                     /gene="glgC"
                     /locus_tag="PLV81_01050"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOM08720.1"
                     /translation="MELQQALKKTLTFILSGGQGERLYPLTKDRSKPSVHFAGTYRII
                     DFSLSNCLNSDLSRIIVLTQYKSLSLDRHIKTAWNLYYRESGNFIDVVPPQQRYDENW
                     YLGTADAIFQNIYIIEHEKPHYTLILSGDHIYKMDYRKMLKYHIEKDADMTIAACEMP
                     LEEAQRFGVLVTDSSLQVTGFEEKPQNPKPVPGKTDVAYVSMGIYIFTTEKLVRYVSD
                     DHKLDSSKDFGKDIIPRIFSTERVFAYDYVQNEGERAYWRDIGTIASYYEAHRDIISV
                     TPILNLYDKEWPIRTYMEQWPPAKFIHNEEGRRGVAINSLVANGAIISGGYVENSVIS
                     PGVKVNSFSEVKGSIIMHGSTVGRYAKIKNAIIDKDNHIPEHMHIGYDLEEDKKHFTV
                     SPEGIVVIPKGYLQ"
     gene            91141..93198
                     /locus_tag="PLV81_01055"
     CDS             91141..93198
                     /locus_tag="PLV81_01055"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HOM08721.1"
                     /translation="MDCNFIFCLHNHQPVGNFDHVFEWAYNDCYRKTLDLLYQYPEFK
                     FAIHNTGPLLEWIERHDPTYCDILAQMVQRGQVEIIGGGFYEPIFSIISEDDIHGQLS
                     LMQSFCKKRFGTEPEGFWTAERVWDPDIPQLVYDFKLQYTLLDDIHFRYAGIPESGLY
                     GYYITERLLRPLRVFPIDKFLRYSIPFKLPHETIDYFKRQCDKYGNCAFVYGDDGEKF
                     GVWPHTYQWVFEEKWLQNFIEAILKEDFITLVHPKDYIKKHPPIDRVYLTQGSYYEMS
                     EWALPAESAAELVRIHNEIKSAGREAEFYPFLKGGVWNNFLNKYHESNAINKRTLYLS
                     REITSLEMKTGKDLSQAKIELYKAQCNCAYWHGLFGGIYLTHLRGALYEHILESEELV
                     CKAKKLHTPEIVTGDFRNEGKDQIFIRNKSCSIVVNPAFGTSISEFSNRIKKVNAFDV
                     IARRKEAYHHLLAELNEDELNNDTVKSIHDMVTVKEKGLKRYLVYDSSRRYSCKELLF
                     TTMPSAEELMFNTIHYTDCSQYPYTYAIHNNSIISDSSGNPLPAITKIIHVHDTDPAI
                     SVHYTIRGFNGIVGIESNVNMRAPHAKECHYIVDGLAQEESYLDSMGQMNNITRYAIT
                     DDIRNVIFSVQASRTATLLRYPIYTVSQSDSGFEKNFQGSCVMLCFEVADTLELDLIL
                     SLE"
     gene            complement(93302..93667)
                     /locus_tag="PLV81_01060"
     CDS             complement(93302..93667)
                     /locus_tag="PLV81_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08722.1"
                     /translation="MDSQEKQPERILVVDEISSILTSDNIVKALAQYKANTSEQEHAI
                     EFVKAHYDFIQEIVTNDIQRKIVRSDFEIKDLVSHVNALMQHKDEYIFTTLVVHSPRH
                     YQQVQKAVLQEMAKEEKEN"
     gene            complement(93906..95126)
                     /locus_tag="PLV81_01065"
     CDS             complement(93906..95126)
                     /locus_tag="PLV81_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08723.1"
                     /translation="MKRHVFWIALIMIISFVACGGSKPQQKASKAGCEMCGEADGFAA
                     IFDNDIALARDRAIDDAMNKLVKMKLGTTVEGRAIVEDYALVESIVEAKSTGMVRNWK
                     VIKEGSQEGAYTVRIYGEVYPQAVNDTIEATLKNYGRPKFMILVRETFEGQQNEPGFT
                     VTELTMMEIMQNAGFEFVDAATVQQLIKKDRAKMMQAMQGQVGGNVQELLLDDVGAEC
                     IIVGDVKTSDQSAVLKQYSANMKSKQAILNLKAIDVYTGSILASTSVNMPGAHIDDAT
                     ASKIAIQRALVKILGSTDEETGKFKSGPFMNQITKKFLLAATHRMITLNIVGLDYNGL
                     TKFRDVLQNRIRGIQKVYVRGQAGKQSKIDVEFSGKTTDLADELVGKSNSIGFKVEIK
                     ETYPNKIMMVVQKL"
     gene            95382..97499
                     /locus_tag="PLV81_01070"
     CDS             95382..97499
                     /locus_tag="PLV81_01070"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOM08724.1"
                     /translation="MSLSSLYDLPVDQITRESYFNVGGMHGFMRWGVYIFLIIAGVYI
                     LYSLIQRVRIWRKGKPELRTDFHEKRIIALLKYVFLQKKILREKYAGIMHASLFYGFV
                     GLFIVTFIILIQEDVTELFFHFKFLVGDAYLLWSLAGDVFGVAVIVGLVMAFWRRYKV
                     KPSRLDTKPIDTFALVLIAFIILTGFLNESLRIAITGFPTFEVWSPVGYTLAHLFGWI
                     SIPTLEVTHYINWWLHMIGAFTFIGLIATDKLGHVLITMLNVYFQNLDNESPKTKFAM
                     PIISPQEFETAESFGVGTVEHFTWKQLMDGDACTRCGRCQDNCPAYLTEKPLSPKKIV
                     NDVKAAMDDRIPKLLVAQDPASVESRALIGEYVLEDEIWSCTNCAACMENCPVQIEHV
                     PKINDMRRYQVLMEGKMAPELQTSFSNMENNSNPYGFAFAARGDWVTEDLGVKTLAED
                     PDVDFLYYVGCAASYDKRNQKVAIALCKILKAAGYKVGILGAEEACCGDSAMRAGNEY
                     LFHSLAAQNLETFKNYGVKKIITTCPHGYNMLKKEYPKFAKVGVDSSGNPLEANYEVY
                     HHTEVIMDLIKLGKIRLSKPLNETITYHDSCFLGRYNEIYDQPRNIIKAIPGTNFVEM
                     SRNHERSFCCGAGGARMWIEEHLGTRINQFRTKDAQATGATKIGTACPFCLTMLSDGA
                     NELDIQNLQTFDLAEYVWEAMEK"
     gene            complement(97557..98081)
                     /locus_tag="PLV81_01075"
     CDS             complement(97557..98081)
                     /locus_tag="PLV81_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOM08725.1"
                     /translation="MPLYEINGRKPVIGEGTWIAPSAEIIGDVRIGKRCYIGFGAIIR
                     GDYGTIIIGDETAIEEGCMIHARPMQLVQIGNRVTVGHMVMIHGSIIMDNAVIGMHAT
                     LSDNAEIGEWAIIAEHALVVNKQKIPGYKVYGGVPAKEIGDVLQKHMDIWNAGKQVYV
                     DLTYQYPQSFKRID"
     gene            complement(98107..99048)
                     /locus_tag="PLV81_01080"
     CDS             complement(98107..99048)
                     /locus_tag="PLV81_01080"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOM08726.1"
                     /translation="MDIQLPLSVRIRLLLKRYLEALNMERRVVVPRYEPDYKPQWGVI
                     IVLFATTFLTTSIAGSSGGDTLLEIIINGLPFSISLLGILMAHEMGHYFAARYFKVIA
                     TPPYFIPFPSIVGTMGAVIRIKSPIPDKRALLYIGAMGPIAGFIISCIISILGLYQST
                     VQPLPQPAPDAIVPVFGDSLLYYLFTKLIHGTIPPGSDVHLSPLAWAGWIGFLVTNLN
                     LMPIGQLDGSHVIYALFGERQRYAGWVFFVILFFLAILWPGWAVWIILTLTLLMIGHP
                     FIPDGRPLNTWERCIGYMCVIIFILTFIPVPVSILGQ"
     gene            complement(99051..101192)
                     /locus_tag="PLV81_01085"
     CDS             complement(99051..101192)
                     /locus_tag="PLV81_01085"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOM08727.1"
                     /translation="MGPLEKLINPSSIAIVGASNNPLKMATMHLLSIMKDGYTGKIFP
                     VHPKEDVVFGLKAYKSPLDLPEAPDCAIFVIPATGIPSTLDAFGTIGTKRAIIISAGF
                     KEVGEEGRKLEEEVKAIASKYGIRFLGPNCMGLINSEIALNTTVMAYTEKPGSLGFAS
                     QSGTYITQSIMYLKHHGIRFSKALSVGNEADISIIDALEYLGNDEQTRTISLYIEGIR
                     DVPRFLEVASNITPKKPVIAQYVGGSTAGARAGSSHTGAMAGPDYLYEGLFKQAGIIR
                     VYSVEELYMHGHVLATQPRLKGNRVGIITNSGGPGTSMANTCEKEGFTVPPFSRQLQE
                     KIKPMIPPHAPAGNPVDITFNLDLDVLTIKIPELAIQSGEVDALCIHGAFRKGFIECI
                     FPHVQQALHLDKPEDVLGFLPSDLDTPGTIPYKYGIPIVMSSFFDYQDDFTVAMQEKG
                     VPVFDSPEKAAKALAVLLRYKEVTQRLPYEKVPLPDVNPQASSIIQKALKNKQKALDE
                     HAAKTLLALYGIPIPDETLIESKKELSAIVDSLKYPVVMKACHPDIMHKTEKGLVILP
                     VKDSNEAKVAFNEIQKRAGFATPVLVYTMVEGKREFMAGAIHQKGFGKAVLFGLGGIF
                     TEALKDITFRVAPLTKRDAFEMLYDIHAKTLLGKFRNEEEVDTSSLANLLCILSTVPL
                     LHPEISEIDCNPIIISGSKPVVIDALVVLNN"
     gene            101420..102184
                     /locus_tag="PLV81_01090"
     CDS             101420..102184
                     /locus_tag="PLV81_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOM08728.1"
                     /translation="MSKLTFDLTGKIAIVTGASRGIGQAIAQTLAAHGATVYCTSRKI
                     EDLKKVQQEIESQGGKAHAVACHIGKLEDIQNLFKVVEEQYGKLDILINNAATNPFFG
                     DVLHATPDAWDKTVDVNLKGYFFASQYAANLMIKNGGGSIVNVASINGIRPAPFQGIY
                     SITKAGVIAMTKSFAKELAPFNVRVNAILPGLTETKFSSVMTQNQEILNMILPTIPMK
                     RVASPQEMTGAVLYLVSDAASYTTGSTIVIDGGALA"
     gene            102215..103486
                     /locus_tag="PLV81_01095"
     CDS             102215..103486
                     /locus_tag="PLV81_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM08729.1"
                     /translation="MAIATMNNETDTISFDQMKTQVNRALEELSIKSNTIVSEPYIGF
                     EKGFTKISVSIPELSPALIIQCLGIIKDHLEHIRIHAFSDGYYAVQALNKNLFKTENI
                     FDNLKIEFSGLTTNFKIECTKKGNLSQEEINCFIALVYKAYSQAKENPVIRLIKYGAS
                     VYTDAGGLDWNYIAGYEEVKRTIRESIILPLKNPKVYDSIAEITRKTFETNRPKAILF
                     EGPPGVGKTTVARIIAKEVQVPLVYVPVESIMSKWYGQSSQNLAAIFDACDDLGGSIL
                     FIDEIDSLAGSRDQNMYEATRRILSVLLRRLDGIGAITNTIVIGATNRKQDLDHALIS
                     RFDQTIHFPLPGEQERASIFGNYAKHLTPEELAKLAAASHSLSGRTIKDLCEQTERRW
                     ARKLIIKDLPPSPPPFEYYYHTVKSFIAAQA"
     gene            complement(103467..105008)
                     /locus_tag="PLV81_01100"
     CDS             complement(103467..105008)
                     /locus_tag="PLV81_01100"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOM08730.1"
                     /translation="MIGDNKSTTKLCIILHAHLPYVRNPLLTLPLEEIWLYQNIAECY
                     IPLLQMCQRLIKKNIIPSVALSISPTLLTMLQQPYYHRRYQKWAHTLLDAISLLRKKN
                     TKACEALDFYNSFITHSLSFFEEINGNCADAFGELFSNNSIELLTTAGTHPFFPLYRT
                     YPSFLNLQVSSGIHTFAKQFMKLPRGFWLPELGYHPGIDKLLYQNAVGYTIVNDASVL
                     FTRNIPKTGNFFPLKTHTGLVLFPRDAVLSMKIWSSHQGYPGNPDYREFHRDAVYELP
                     ELSPNNEQRLLGLKIYAISGSDNKEYYNPQKAMAVVQQQVHDFIDAILKRAQEVESVI
                     KRKPVFVLPFDAELFGHWWFEGPLFLEMLLETIASRDDIMCVMPQQLLDCDMETFEPV
                     ESSWGRGNDFSTWYNPKVRHTVVKLEELLYRFDKALYSNDEALQQCARELMLASSSDW
                     QFMISTGSYADYARERFEEHSAAAQTILDMIEKKITNNSYINKRFETYPVFEHIDSLL
                     RLVQQ"
     gene            complement(105005..105430)
                     /locus_tag="PLV81_01105"
     CDS             complement(105005..105430)
                     /locus_tag="PLV81_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08731.1"
                     /translation="MSGYFTVHLPSFSLMHVHWAIDKEYWDTIVSHAVTDVYGDPVLF
                     IRIVKLHGNEWILDQQIDVFGRNNHWIVSIGDNLHGCTIKVMLMTVIEGKGEEIIFES
                     DVIMLPLSPENLKDTIPTFTQEIHTLNTIDLANEKRLSS"
     gene            complement(105441..106469)
                     /locus_tag="PLV81_01110"
     CDS             complement(105441..106469)
                     /locus_tag="PLV81_01110"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HOM08732.1"
                     /translation="MQQLISKKGDLLGKDGQLIEKGYSISPVRTYNPENVSLYPVSWL
                     NKIRIKEWDYYGITTKDYFVSATISHIGYAGLVFVYFIDFKTKQYIEDTLITPLGKGF
                     TLGRSSDDDSYFKKDDITFAFRKKGNEREIYVSWPQFGKGKGFHAHWIIRQTPHDSIV
                     MMTPIGKKRFYYNQKINCMPARGAFLLGGQEYGFDSGNALATLDWGRGVWEYKSFWNW
                     ASASWFLPDGTPFGLNLGQGFGDLSYATENCFFVNGRIHKLDKVTFNYDNSNFLKPWY
                     FGSNDGRLGLTFSPFFERVATSNLLVITSEVHQLFGIYNGFVIDNSGKKIQVANIIGW
                     AEEHHARW"
     gene            complement(107237..108694)
                     /locus_tag="PLV81_01115"
     CDS             complement(107237..108694)
                     /locus_tag="PLV81_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOM08733.1"
                     /translation="MCGQQFTYNLYELFQEQAHKFPKKIMLHYNNVPVLYKDALHKVH
                     KIAHALRKRGVHSKTTVIIQMGNTPYFVYSFFAILQCNAIPVLVNPLARQFELHHYIT
                     VTRPDCIITTSFMAERLLQHGIVSDIHTIITIDESSTYTSMDSIVGSDTYDNHYVQEN
                     NDAAAIIFTSAMDGYAVGAVLTHYGIYQSAKAVAQMMPGHALVSVAVLPLFHAFGLTS
                     SLVVPLLKNIPIELVEKFSLKKLIRILSNNEVRSFVGVPAMYAIMKGLFERGNNFGHI
                     QLWVSGGDYLPVSLQKWYAERQVDIRQGYGLTEASPIVSWNMPEIENKIGSIGKPMPY
                     NKVRVVDDSCRDVPACMNGEIIVKGMNVINRYYNNPQKTKEYIKDGWLYTGDIGYFDN
                     DGYLFISGRKKDMVLKNGYNVYPKEVERLLSYHPDIESVRVTGHVEFLGDSTREWLIA
                     RVKLKHHATLTADTLRMWCSENISLYKIPDEIIIE"
     gene            108887..109330
                     /locus_tag="PLV81_01120"
     CDS             108887..109330
                     /locus_tag="PLV81_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08734.1"
                     /translation="MIVLTFIIILQISLAVHVYYIISYISKKQDKDFKGFLFTSMINI
                     FLGIFLSVFILLFPQDLKAINLERILFIESGVIFFGMLYVKTKIAVNIYKRTKDPQYF
                     HYSYFGKKVLHPNVVKMPELFTYFLTLPLTLVCGAYFIVKLGCGK"
     gene            complement(109335..110444)
                     /locus_tag="PLV81_01125"
     CDS             complement(109335..110444)
                     /locus_tag="PLV81_01125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOM08735.1"
                     /translation="MKPLVNQERLVSTFIDLAKIASPSWEEKGVIEYIKQKADTLGVE
                     CALYPCNNSYNVLIRIPGDKSYNPVLFSCHMDTVTPCENIQPIIKDNKIVSDGRTILG
                     ADDKSAIAAFLEAISLLQEHSLPHGHIEFLFSCAEEVGLFGAKGFDLSVVKAKYAFVF
                     DSDGPVGKIIVAAPYHISMKIIIKGKAAHAGMEPEKGISAIRVLSEIIAAIPHGRLDS
                     ETTVNVGTVKGGRATNIVAEEAECTLETRSRQKTRVLSVKREIERTAKTIAQKYKAKV
                     KIESTMDYEGYTIRDNDPIVLIASNALRSIGIKPALAVSGGGSDTNIFNAHGIKAINL
                     SSGMRQVHTTKEYIIIQDLVNVASLVVSIIQNVKH"
     gene            complement(110453..112936)
                     /locus_tag="PLV81_01130"
     CDS             complement(110453..112936)
                     /locus_tag="PLV81_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOM08736.1"
                     /translation="MKAIIMAGGEGTRLRPLTCNRPKPMIPVLNRPVVEYSVYLLKQY
                     GINDITMSLFYLPENVVNYFGDGTFWEVSIDYSIEELPLGTAGGVKLALKDYDDTFIV
                     LSGDGIYDFNLQDIIDFHKKNKSPVTIALTHVSDPIEYGIVIARDDGTIERFLEKPSW
                     GEVFSDTVNTGLYIIEPEIMERFVPANEKFDFSYDLFPLLQKNNIPIYGYISDGYWCD
                     IGNLEAYRKVHQDILNGMVKISFPGKKIGENIWAGKDVEIHPEATVTGPVVLGNYVKI
                     KKGAEVSDYSVIGDNTVIHENASVRRSVVLHNTIIGTKTEIRGAIIGKRNVIEDTVSI
                     YEGAVISDDCQIGNNAKIASGIRIWPEKIIDQGTQLNSDLIWGKTEKKILFSAEGVYG
                     SFNIKITPEFAAKLGSAIGAFLGKNKKVIVSRDTTRASRLIKRALTAGVLSMGVEVYD
                     LEIASIPINKYSVVYENADLGIYVQISPHTDLQFNLIRMFNKQGFELNNVERRKIENI
                     FFRGDYPRSSAFEVGRLYYPTHNVESYIMFVSNFVNKEIIAKKQYNVIVDCFYGTTTH
                     IFPSLLAAFSCTATVLRGQIREFNSTEEIRNESRDSINGIKNLTKLNNEIGVIVGPHG
                     EQLTVVDEDGNILQWDDITTLLGLLFVKYRTIKELCIPVISSSRLGKIAEENNVILKR
                     VPKTPVFEDVFTIFSKNSESVYPHSMYEYDPMIAFLLLLELVAREEKKLSEIRRELPD
                     TNMYHTVLPCPVDAKAVVMREIASTANGHVELIDGVRVVHDDGWIIILPDTLQPSVHV
                     YCEGITDEARDKLIDDYSIKIKKITAKYA"
     gene            113235..113948
                     /locus_tag="PLV81_01135"
     CDS             113235..113948
                     /locus_tag="PLV81_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOM08737.1"
                     /translation="MSNPIKESTLERWIQNQMKVWEAEKKVIEKEDVPRPFITISRQY
                     GCFANDIAQAIATSLNDLQRSDKWITYDKNLINKIEEQYNISEKIVETIDTKKREEMS
                     ELWRTMLSDLPPQAAVFQKLANTIRSLAIHGNAILVGRGSVIITKGLRFGLHVRFVAP
                     LQYRIQKVMEIQKIKDRLTAEKLIEEKDKQRHEFMTQYIKFDAYDPASYDLTFNIAQF
                     TPDQIASIVIAALKARKLI"
     gene            complement(113995..114585)
                     /locus_tag="PLV81_01140"
     CDS             complement(113995..114585)
                     /locus_tag="PLV81_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08738.1"
                     /translation="MKKNFLTIIMVLFTSVLLAQTTQKLDIPVQSLYSNQVKIKDIYF
                     NRRVDIAGKGDVLEVEVLFENLTDYPMDIYVNIIATYEKEEKTKSSFEMPIPEKERIR
                     NFVPYPDDIANYEYTDSKGKKIFQAYPKNTKSGINPLTNKLYFLPVRDILVVRSYHVS
                     PYRKDFIFFNYVTVLVFDKDGNPIMREVYELKGKRK"
     gene            complement(114661..115446)
                     /locus_tag="PLV81_01145"
     CDS             complement(114661..115446)
                     /locus_tag="PLV81_01145"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOM08739.1"
                     /translation="MDYKAITEYAIHVCKQAGAIILEGYYSNDVTVDYKSRTNLVTNI
                     DYESEKLIVESIKNTFPDHGIVAEEGSLKKSSSNYVWYVDPLDATNNFAHKIPHFCVT
                     VALYSREIASTVAGVVYEPLRDECFYAWKGGGAYLNTKRIYVSQIQDIGISIIATGFP
                     YKKDDPDVNNLKQFNNVLPHVQGIRRMGSAAIDLSYVACGRFDGYWEPMLYPWDVAAG
                     ALIVEEAGGMVTKYTGEAFDPEYPEICATNGLIHRQLLDCINA"
     gene            complement(115449..116879)
                     /locus_tag="PLV81_01150"
     CDS             complement(115449..116879)
                     /locus_tag="PLV81_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM08740.1"
                     /translation="MYKKIITVLFCICVTALYAQDPEELTREGVELGRKQKFDESLQK
                     LDESIKLYNVRSAKTLHNMGYVYELKGEIDKAIPAYEEAVRRNPVQIPSLERLGYLYY
                     KTGLFDKAVATGEHVLKLDPKNQEVIKWLPDAYKQKLIAQAKSQMEEAEKEEKKVTKE
                     EVVEEEKPQEKKRVLFATFEGMSRIGYFLDGGGFEIVSDNGIGPNMPYLFYIDAGPFD
                     GWEARITTGNPYYGALMPDVNAWMEKVEGMFNAGGYYLGFGVLGNHHRSGDVFGENKH
                     LNDYKMGVLFGFTQDKARVDIAMYPRLIMRDGEKSSGKTFDADYFDFVYTYTFDKYLR
                     YYTKLTINEFYFFDHTVPACLYAGVYDFSIGVVLNRYNELTGQKRLSFNFSLTERMYF
                     GNINIDDPYAKFGNGQGWFGINTDKWTKGDPFSGFRSLSHVFTASVEEYIHKNIFLYQ
                     SLGFELADTSEDHHEITFKLGAGVEL"
     gene            117063..119324
                     /locus_tag="PLV81_01155"
     CDS             117063..119324
                     /locus_tag="PLV81_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HOM08741.1"
                     /translation="MKKTIMVIGGGLLQVPVIQTAQKMGLQVIVTDYNPNALGMKYAD
                     IPIVMSTRDIEGSVRVAKKQHEITPISGVLTVGTDASATVAAVANALNLPGIKFEDAI
                     AATNKIKMRTRFFEHGVPSPHFFPVWSLSDAKKACKVLGFPVVIKPSDNMGARGVVRI
                     DNKNQIADAFAFAKSASPSGELIIEEFMEGHELSIDAVVFNGEVTFTGIADRIIEYPP
                     HFVETGHTMPSQLPKEVQDSACEVMKAGIKALGIHLGCAKGDIKITKDGPKIGELAAR
                     LSGGFMSAYTYPLSTGVNLMRAAIEVALGQEPGNLEPLYNRVSIERAIITKPGIVKKI
                     CGVEEALKIPGIAEIFITVKPGDKVKKPTSNVEKAGHIIAVADTLKEAEEAVRKCKEM
                     IHIEICDESELSMDTINISARERLRKVCYVCKQCNGKDCASGVPGMGGAGSGASFKRN
                     SEALLNYKINTRVIHDVTNPDTSTSLFSINLSFPVMAAPITGAITNLGGAIDELDYNI
                     AVVKGCMDAGTIAFLGDGADPEKYKIAMEALSQFGGMGIPIFKPRANNEEIIKRIKAA
                     ETAGAIAVGMDIDAIVFKTMELKHQAVKPKSLTELKSIISSTHLPFILKGIMNPRDAA
                     MAVEAGAHAIVVSNHGGRVLDEMVGTMDVLEDIVREVHGHIHILIDGGFRTGVDILKA
                     LALGAEYVLIGRPVAIAAVGMGSEAVAYYLKSLQAELKKAMILTGCATIHDITGDIVR
                     KIHHNSYQPLEVR"
     gene            119554..120003
                     /locus_tag="PLV81_01160"
     CDS             119554..120003
                     /locus_tag="PLV81_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08742.1"
                     /translation="MIKLSRWYIAIAIVTVTIATHALQFTDIHGIWQLLYRGNYGYEF
                     RFYKNYQAVCIIYMQTNAVVFKGVYTFDNPQTIRININQMKNADSCCNVNTTTGFAQV
                     KSSYFLFNATLITKNNKKMLELKPLSIVIDGINSEGYFEPVILLQRM"
     gene            120009..120746
                     /locus_tag="PLV81_01165"
     CDS             120009..120746
                     /locus_tag="PLV81_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOM08743.1"
                     /translation="MSYRTITIPYKDPIDAVVYPCSQSVGTIVYSHGLFSSKDAYKIH
                     QIAPALHDSGYTVISFSYGYTRHYTTGNALTIALPQCADELNAVLEYAANTYNPSLHI
                     IGSSMGGAIALYYAATVPYIFSLKSLSIIATPVHINHLVRAIAPQGISGNYVTVGEYT
                     IDAQSLQQMNTINLIKLIPRVDIPVCIIHGQNDDVVPVAHAHDIQKNLCVPHTMHIIP
                     DGDHTLTAQRHVELILQYIVSWLKLHP"
     gene            120863..121726
                     /locus_tag="PLV81_01170"
     CDS             120863..121726
                     /locus_tag="PLV81_01170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013447291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOM08744.1"
                     /translation="MLTNLNQCPNPIEYQNQNINEDIFNYLTEKLKEKTILHYKKAIL
                     LQGDVLNKDYFDNDIFDLIVTSPPYNVDIKYNTHKDNISYEEYLQFSEAWMSNCYKWS
                     KKQARLCLNIPLDKNKGGQRSVGADLTILAQKVGWKYHSTIIWNEGNISRRTAWGSWL
                     SAAAPYVIAPVELIVILYKEEWKKTQGSKISDISKDEFMQWTNGLWTFNGESKKRIGH
                     PAPFPLELPYRCIKLFSFVHDIVFDPFAGSGTTLIAANNINRYSIGLEIDSSYCELSK
                     QRILQENGMLF"
     gene            121742..122326
                     /locus_tag="PLV81_01175"
     CDS             121742..122326
                     /locus_tag="PLV81_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004275842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOM08745.1"
                     /translation="MQKQKTITDLIIEFFKNHPKEDMPHGPVVDWVEEQYTKLYNRKP
                     RDIWRSIRRLHQEGFLIKVKKGIYRYDPDIATNKHLEEFSQELKNQILERDNYKCVIC
                     GRTAKEGYELHVDHILPKDKGGKATLDNGQTLCSICNFRKKNYNQTESGKRMFIRLLK
                     IAKEVGDIKTQKFCEEILNTYEKFNINGHIKWDK"
BASE COUNT        38239 a        24182 c        22190 g        37535 t
ORIGIN      
        1 tgaaagctac tttggtgggt caagggctta ctttgacagg aactatcgcc ctactgtaaa
       61 gattacactc catcaatact ttttagagtt aggtaacaca ttggaacaat gtattgaacg
      121 tctgtgtgct tattgccgtg gtgagctttt gctctgaccc tttgatcttt gataatacgt
      181 gtatacccca cttgcatagc agagtataca cgcattaaaa taattacata cccaacattg
      241 ctttcttcag gctttcctgt acaggctttg gtccaagcca tattgcaaat aatgctttct
      301 taagatcaag cccggcaaca gaacccaatg tggttgtttt ctttgttgca gacgcagtaa
      361 atactgctgt aattcctgtt tttggagcat atcccaattg aatcacatca ccttttttga
      421 attcaacagc atcaaaaagt tttaagaaag cgtcctgttt ggcagttgca tatccacttg
      481 atgcagcctg tgcaaaaccc tcttttgtag cttcaaggaa tctttcttta ttaagaagca
      541 cagagtcaat caccagcaca acagccattg gctcatcagc ttctactatt gcctttgcat
      601 cggctgtttt aaggctttca ggtacccaca atgttgcata atacactgta cctaaaatcc
      661 cctttgttct tttgccagta cctttcttca ccagatcttt tcctccaaga ctcagcgaaa
      721 aaacatcaga catcatgcaa acagcaacca gtaatgctat aacaactact acacttttac
      781 gcatgtttaa cctccttgcg ttatggtaat ttttttatta catatagttg atggtcatag
      841 agtacttaca ttgtatttat acaatagtca attaaaattt ttctgttcaa tgataattga
      901 cagtgtatcc ataacatcaa aaaaatcatc ccattcatag tattctttat cttcaggcaa
      961 aaactttttc aaaagacccc tggcaaacac aatatcagca tactttgcca tctcaatatc
     1021 ggttggtcca tcgcctatgg ctattttaca tgtgtggcga tagttactca taatgttagc
     1081 ctttgccacc aactcacctt catgctcata atcagaatac actttgagat atttaccgct
     1141 agcatcaatt tttgctgcat agattgccgt aaccttattt ttcaaattgc ctaattttcg
     1201 gaccacacta tcgtacaatc cgcctgatat gataataaca gggattgatc tgttattgca
     1261 atattccaga aactcctcaa atcctggccg tatttcttta tcatcaatat aagcaagcat
     1321 gtctgaatat aatgaagagg gcaacgattc aagtagctgt cgcacaccct gcctgatggt
     1381 aagctcctta ttccacatct ttttgccaat ttcatcaaat aaacctggag caaaggtttt
     1441 acacatacca acaaatgtct caacggtggt tatcgtgcca tcaaagtcac agaatataca
     1501 atatgtagta ttggcatgtt gcattatttg atggttcttt cgttacgtta tgttatatag
     1561 tatccaatga actatcgccc cgcgattttt ctaaaagcgc tgttgcatta tccaaaatac
     1621 ttcgggaagt gtgtgccagc tgctgtatct catcaaccat ttgagcaaca acttcattca
     1681 tatgttcaat ggcctttgcg cttgtttcaa ttgcagcttt ttgttcttca gtgccagcac
     1741 caatgttttt tgccagttca acgttctttt ccatctggtc aataatattt tttatatact
     1801 tctcctcaac ggtaacacta tcctgcaata ttttaatctt tgaggaactt tcagccatac
     1861 gggcaatcag atcctttaac atttcagcag ttgtcctgat gacctcaaca ccagtttcag
     1921 tggtttttgt gctctgtatc agcacttttt caatatcttt gatgctctcc gatgtttgaa
     1981 atgccagctt gccaatttca tccgcaacca ctgcaaatcc cctgccatac tcacctgcac
     2041 gcgcagcctc aatgctggcg ttgagtgaca gtaaatttat cttatctgct atatcaacta
     2101 ttacgtttat agtgtcacca attttctgac tttgctcccg tatctgattg atggttgctt
     2161 caacctcttt gattctttcg ttagcattgc ctgtacgcgc aacaacattt tcaacttctt
     2221 gaattgtttt atctaaattt tgccgcgttt cgttttttat aactttaaac tcgctgacaa
     2281 tggtttccat catttcatta ccgcccacct gctcttcgga aatgcgtgct atgctttccg
     2341 ctgagcccag cagttcttcc agtgttgctg atatctcttc aaatgttgat gcctgattct
     2401 gcatcttttc gttaaatttt atagtgagta tatcctgctg ttttacctgt tccagtaaat
     2461 ctgctacctt ttcctttata gtctcgttga gctgtcgctg catatccgat tgctgctgaa
     2521 taacagaaag ttgtctggtg gagcgatcat catattgctg tactactttt atattgcgtg
     2581 aagctaatat tccaaacacc gctgtaatca tgataaagaa aatctcgcga aatataataa
     2641 agccgtggac tggcttgcca tcaagtgttg catcccagtg tataaccgct ccatgagcta
     2701 tcgccaccaa taaaaatgca gtccatgtta ctacagtata gccagtgtaa aaggccataa
     2761 gccgtcggtt gaagtacagt tgcaacataa ccagtaatat aaccataaga actgaactgt
     2821 tatacgactc aagtgcaaat gtccagtcat acttttgcgc ataggcaaac ttggctgcta
     2881 aaggtatatt gacactgata aatcccagaa tccattcaaa cacgccagtt gctttcagta
     2941 atttttttct tttataaata acatatgcca gtataacaca gatagtgctg attaaggata
     3001 atcgtaaaat ttccagaata tcaactttcc ccaatatctg gaatatatgg gcaatgacag
     3061 cgattataat aaatataata atatatgttc cacttgattt tatttccaga acctgacggg
     3121 ctgtatagtt tgtcttcata ggaacctcac aaaatgttat tcaaaggctc tcagtactta
     3181 tagtatacta tgtagatagc aacttcaaca ataataatac tttagtgaca ataaattgct
     3241 ataaaatatc accatgtcta tcagtacaaa ttagcataat catattttac attatatata
     3301 atatagctca taccgtccaa ttatccttat attttagttg aaaaaactcg cgctatattc
     3361 agtcctttca cacaatgtaa gttctgttat attcaaccca aatgatattc tctattcttg
     3421 catctatcat gaaattggta caatgctatt cagaaatttt tttataaaaa catttgcatt
     3481 tttatataac ctgtacccca ctattggtga atattcaaaa ggattattct ttatacatat
     3541 tcctttctct ccatgatttg gagtaacaac gaaattttat gtatggacta aggtaaccct
     3601 ttcatgaata ccattaacat tgcaactctt aacaaaaagc gctatcttca tgtattgctc
     3661 cctctttttg ttggctcaat cattgcatat ttagacagag tcaacattgc gtatgcagca
     3721 cttaccatga ataaggattt aggattttct gctgagatct ttggcatggg tgccggcatt
     3781 ttttttgttg gctatattct ctttgaaatt cccggagcaa tcattgccga aaaatggaat
     3841 cccaaaggat ggctcatgcg tatcatgatt acctggggta ttgtatcatt tttcatggca
     3901 tttattacca aaccatggca tttttatctg ctgcgctttt tactgggtac tgctgaagca
     3961 agcttttacc cggtattata tgcaagcatg ataccgcgct ggtttacacc agaagagcgc
     4021 ccaaaagcta ttgcccttat gctgacatca cttcaggtat cagcaattat tggatcgcct
     4081 cttgcggggt ttttacttga tatatcagtg tttggcctta aaggctggca gcttttattt
     4141 gtatgcgaag caataccagc actgttattt ggaatacttt tatattttat cctttcaaat
     4201 gacccattgc atgttacatg gcttactgat gcagaaaaga aatatttatc agatacgtta
     4261 tctcatgaat taaaacaaaa agggcagcat tacaccgtat ggcaggcact caaagataaa
     4321 gaagtattga aactggcact catctatttt ctatggataa ccggcttctg gggtttcaat
     4381 tactggatgc caactgtcat caaagaatta tcaggctggt caaatattgc cattggcctt
     4441 ctttttgcca tacccatgac attgtctctg attgtcatgg tgtgtattgg tcattcatcg
     4501 tcaaaaaccg gtgaaaagcg cctgcatggt gccctaccgc ttgtaattgc ttccattggg
     4561 cttggtgtgg gtgtttttat caaggaccct gcagttaaca tgatttttgt ttgtctggca
     4621 gcaattggtg tctttggtcc ttttggtgtg tggtggtcat atcccacaac atttctgtcc
     4681 ggggcagcag ctgcaggagc tatcggcctt atcaattcat ccggaaatat tggcgggtat
     4741 gttggcccat accttatcgg catcattaag gacattacca attcatttaa agtatcattt
     4801 gtaatacttg catgtatggt gctgacatct ggcatcatga tgctcacact gaagaagaag
     4861 gtgtaaccat gaaaggtttt gtgcaatgtt ataccggcaa tggtaaaggg aagacaaccg
     4921 cagccattgg gcttgctatc cgagcggccg gctggggtaa gcattcaatt atcattcaat
     4981 ttttaaaagg gcaaaagtca ggtgaacaga tagcactaaa gcgatttaac gatttaataa
     5041 caattgaaac atatggaagc aaaaactttt atattcccgg taacaatgat actgaacata
     5101 aaacgttagt gcataaagca tacaaccgct gtatggaagt ttgtggcgat agttcctatg
     5161 atataatcat ctgtgacgaa atacttacgg cgctgcattt caatcttatt gcaatgaacg
     5221 atattattgc gttgatacaa gccaaacgaa ttgacgctga acttattctg acaggaagag
     5281 gattaccaaa aaaactgttt ccatactgtg acctcataac agagatgaag gatattaaac
     5341 attattatga gaagggaatc aatgcacgaa aaggaataga atggtgagta tataatctaa
     5401 cggcgaagtt taagcttcgc cgttagattg gtatttcgtt tacttgccct ggagtttctt
     5461 tgcatcattg atgagtgtat caacaacagt tgggtctgcc agtgttgaag tgtcacccat
     5521 acctgatttg tcaaattcgc ctgctgcaat cttctttaag attcgacgca taatcttccc
     5581 tgaacgggtc ttgggaagtg catctgccca ctgaatcatt tcaggtgttg cgattggtcc
     5641 aatctccttg cgaacatgag caacaagctc tttcttgagt tcatcagttt tttctacacc
     5701 tgtcttaagg gttacaaatg catagattga ctgaccctta atttcatgtg gataaccaac
     5761 aactgcagcc tcagcaactt ttggatgggc aacaagtgca gattcaattt cagcagtacc
     5821 catacggtga ccggatacat tgatgacatc atcaacacga cctgtgatct ggtagtaacc
     5881 gtctttgtca cgcttacagc cgtcaccagt gaagtacttg ccagggaact gtgtaaagta
     5941 tgtattgaag aatctttcag gatccccata tacacctcgc atctgtcctg gccatgaccg
     6001 cttgatacac aggttacctg agcatacacc ttcaagttct gtgccattat catcaacaat
     6061 acatggctca acgccaaaga atggaactgt tgccatagcc ggtttaatgt caattgcacc
     6121 tggtagtgga gtaatgagga ttccaccggt ttctgtctgc caccatgtat caactattgg
     6181 acactcaccc ctgcctatct tatgataata ccagttccat gcttctggat tcaatggctc
     6241 accaactgaa cccaacaact gaatggatga aatatcatgc ttgtcaaccc agctgtcacc
     6301 ttcttttgcc agtgcacgga tagcggttgg agctgtgtag aaaatattaa ctttgtattt
     6361 ttcaacgatt gcccagaatc taccaaagtc tggatatgta ggaacacctt caaacatgat
     6421 ggtggttgca ccatttgcca gtggaccata tacaatgtaa gagtgccctg ttacccagcc
     6481 aatgtcagcg gtacaccagt agatgtcacc gtcatgatag tcaaaaatca ttttgtgtgt
     6541 gaatgcaaca taggttaaat aaccaccggt tgtatgcatt acaccttttg gctttccagt
     6601 tgaaccggaa gtatacagaa taaacagtgg atcttctgca tccatctgct ccgctgggca
     6661 gttggcatca acctttgcca tttcttcttc ataccactta tccatcttgt tattccagtt
     6721 aacctggatc ttgtcgccaa cccgttttac aacgatgtgc aggcgaacag ttggacactc
     6781 agcaatagct ttatcagcag tgtctttctg tggaactgct tttgcaccac ggaaagtacc
     6841 gtcgcaggac acaacaactt ctgatttaca gtcctgaatt cggtcacgaa gcgcttcagc
     6901 tgagaaccca ccaaatacta ccgagtgaac tgcaccaatt cgtgtacatg ccaggatacc
     6961 aatagcaagc tcaggaatca ttgggaggaa gaatgtaacg cggtcacctt ttttaactcc
     7021 atttgccttc aatacattgg caaatttaca tacctgctcg tgcagttctt tgtaggtgaa
     7081 ctttttgtct tcggttggct cattgccttc ccagatcaaa gcaacctggt tgccgcgttt
     7141 ttcaagatgc ctgtccaaac agttgtatga aacattaacc ttagcattga caaaccatcg
     7201 tacccacaaa tcctttgctg tggtaccata gttgcaatcc attaccttgt cccatttctt
     7261 gaaccaggta acatactctt cggcaatctt tccccagaaa ccttctggat ttgccagtga
     7321 ttctttccac atcttttcgt actcttcccg gctctttata tacgctttct gcctaaattt
     7381 ctctggaacc gggtaaatct cttttaatgt aacttctgcc atctattacc tccgatgttt
     7441 atattgtaat atgcacatta tgggcaccgc tagagtgcgg tgtaacccct acgcattcct
     7501 gtattgttct ttcaaatatg cagctatcat tgcatcatag cctgatgtca actcaaaaac
     7561 cttaattgcc agcctgaaat ttgtcttttc tgatacagca ccgctattag cttccatctc
     7621 tttaagtacc gtttcataat cagatgggtc aacaatgacc gttacatatt tataattttt
     7681 agccgatgag cgaagcattg ccggcccgcc aatatctata ttttctattg cttcttccag
     7741 tgtgcatcct tttgcaattg tctgcttaaa tggatataag ttgataacca ccatgtcaat
     7801 gggaatgata ccatgcttct gcattgctgc aacatgttca ggattatcac gcaaacccag
     7861 aagagcccca tgtatctttg gatgtagcgt tttaaccctg ccatccatca tctcaggaaa
     7921 accagtgtaa tctgaaactt cagtaacagc aacaccagaa tcgcgcaaaa gctttgctgt
     7981 tcccccggta gataaaatat caacaccaaa ctctttaagc tttgaagcaa acggtactat
     8041 tcctgattta tctgacaagc ttattaatgc tcgtttaatt actgacattt taaccttatt
     8101 taactatttt aattatacta aaattgcttt ttattatcat actacggtta tgaccatttg
     8161 aataagcacc tgaaaaaata caagcatttt ttaaatctga acaaaatcac tgtattttaa
     8221 caataatcaa gacaacggat agcataattt tgtgtatgtt attattaaga taaccatcaa
     8281 ttcttcatca ttattgatat atttataata gttaatgtgt tacacaatga aaaaaagcat
     8341 tggaatacct atattgtgct tttccctaca ttacatatat tgctctgacc ccaacgatcc
     8401 caactgcctg gcactctccg cattccccgc gtgatgcatt cttgtacctc acgtaaagtt
     8461 cgcttagagt gcaaagtttt gatgctctga cccttcatca ggctttttcc cgataactat
     8521 cgcacagaat gtaaaaaaat tacataaact gaaaaatttt gttgccattc ttataaaata
     8581 atagtatttt tatactaaaa tagtataaaa gtataaaata ttatgactaa acgaaaacaa
     8641 caacagattt taaccaccgc cgaggcgatg tttaaccgtt ttggcataaa aaaaacggct
     8701 attgatgata ttgcccgtga tgccagggtt gctaaagcaa ctatctacaa ttattttggc
     8761 aacaaggaag gcttgctcaa ggccttaatt acacaaaaaa tacagcaatt tgaatcgcgc
     8821 ttactgaata ccgctgaaac agccataaat cctgttgatg caatcaaatc attaatccag
     8881 gaacatatac gcctttttac cagtaaccct ttcattaacg acccagcgct acaggataac
     8941 gaattaaaag aagaactcaa ccaaaaggaa gaacatctgc tacaaaaaat agtacagcac
     9001 gcatcacgta agggtttatt acaaagcggc gatagtaacc agataatcaa tacgctgctg
     9061 ttactgatca aaggcctgga atcatccatt aaaaatatct ctttttcgat ggattcattg
     9121 aatgacagca tcaattttat ttcagagaca ttctttaaaa atacggcaaa ggagggacat
     9181 aatggcacag cttaatgtga tgctgtttga attcattgtt ttttcagttg caggatggat
     9241 agtagaggtt atttaccgct cacttaccaa taaacggttt gtaaatcctg gctttttgac
     9301 aggtccatac ctgcctattt atggaactgg cgctctgatt attacctcta ttgcgtatgc
     9361 taccatacat attcctgtag tatataaatt tgttatttat tgtatagctt taagcctgct
     9421 tgaatacatc accggtgcta ttctgtatac aacaactggc aaacgatact gggattacac
     9481 tgatgaaccc ctcaatatac agggacatgt atgtctgcca ttttcggtta tatggggtat
     9541 gttagcatta gcatttgaat atgctgtttt gccactgtat ggctattttg tttcatcact
     9601 gaatactgtt atcattaata ctgcaataag ccttggcatg ttactcatga ttaccgattg
     9661 cgtgctgaca cacaatattg tacaaagatt actgcatgcc cgattgcctg tatttaatcc
     9721 cacacagtac aggttaggac ttattaaatt tcagaatgat gcgctcattt cattgcgtgg
     9781 ctttatggat actgctcttt catacaggaa aaaattttct actataaagc ccctgaacgc
     9841 aaataaaatt attcaattat tcaaaaagag atatactgca ctaatcagca ccattcgtaa
     9901 taaaaacgaa aatcacaggt aataccagca tacaggagaa ttatggaaaa taaaaggttg
     9961 ttcttattgc catttgtaca ggatatactt cacaatgagg attttcaatc actacggcgt
    10021 tacacacatc atggcacaac aacatgctat caccacacac tggaggtagc atggttagcg
    10081 tatagattag ctgtagcttt aaaacttgac catgttgccg caacacgcgg ggcattgttg
    10141 catgatttct ttatgtacaa ctggcgcaca ggtggcccaa gacttcatgg attcaggcac
    10201 ccacgcattg cactcaataa tgcatcaaaa cattttgtgc tgggtccaaa ggaaaaagat
    10261 agtatcctgc gccacatgtg gccgcttacg ccacttccac cccgctatcc tgaatcatgg
    10321 atagtaacag tagctgataa gatagtatca actatggatt actttcgctt tgtaaaaagc
    10381 aaagtatcgt tgatgtataa taccatacac acctgcatgt ggttaccatt actatcgcga
    10441 ttcaatcgtc agaagcagta ttcttaaatc actttattgc ttactggttt gttaaaacgc
    10501 acgtgttccc tcacgtggca tcatagcagt ctggatgatg ttcacacgct acgtttgcag
    10561 gaactatcgc cccctgtaca caatcaaagc gctaacgggc aggcctccat gactgaaatg
    10621 tatttcctta tcagtaggga tagtaactat gtgtttaatc tttgaaggga ttaatacaca
    10681 aaaactccat gctggatacc atttgttaaa aatatacact agtcgtttat ataattgtat
    10741 agtatcatca gtatgaatac gcttcccata gggtggattg atagtgcaca gaaccttacc
    10801 gctaccaatc actttttttt cttttaaaaa atcgccagta tacgtatgca gctgggcacc
    10861 tgctttcatg ctgttttgtt gtgttatagc taccatatca gggttactgt ctccagctat
    10921 aattgttata tccctgtgct cacctgactc gttatgcgta ataccggtga atgcatgctt
    10981 gcggaatgac ggccagctca taaaggcaaa tgtacggtta gctcctggtg gaattgcatg
    11041 aagcatgcac catgcctcaa tggggaatgt gccactgccg cacatgggat caatgatggt
    11101 atcgtaatca tacacattac attcatataa tagtgctgca gctattgttt cacgaagtgg
    11161 tgcctcactg atatgctgct tatacccacg cttgtataac ggttcaccgc ttgaatccaa
    11221 acttaccatg cactcatcat ggtcaaagcg cacaaatacc ttttgtactg aatcagcatt
    11281 gctcgatatg gcaaacttgg acaggcgttt ttctattgct tttattacct catggccaat
    11341 tcgttcagta tgatgcagct ttgatttatg gacacttacc tcaacatcaa ttgtgtgtcc
    11401 ctgtatatat agttcccacg gaaattcttt aagtgtgcga tagatctcat taaaatgata
    11461 tgccctgaaa tggcacaacc gcatgagtat acgcgttata acccgtgaat agtagttggc
    11521 tgtgtaacag tgttccagcg ttcccctgaa tgtcactccg ccttttgtca ttgcacactc
    11581 ttttatacct aaaagcgtta gctctttatg ggctgtttct tcaaannnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnttgca cactctttta tacctaaaag cgttagctct
    11761 ttatgggctg tttcttcaaa tccgggctgt accactgcaa aaaatgtatg ggacttgtgg
    11821 atcacatgct gagtaattcg gcgcttgata gcattctgtg aagtgctgta attcatggtt
    11881 actttttgtc aaacgctgca agcaattcaa gatggggtgt ttgcggaaac atatcatagg
    11941 ctttgcacca tgctattgcg tatttgtcac agagcatttt agcatcctgg gcaaaggtaa
    12001 caggattgca tgacatatac acaatccttt ttaggtccgc ctgcaacaat ttgttcacaa
    12061 ctttagggcc aaggcctgtt cttggtggat caactattgc tacatcatac ttcactgcag
    12121 tatcgcttat atactcaaac gcatcaccct gtacaaacaa tacattgttg attccattga
    12181 aggtggcatt gtgccctgct accgtaactg cttcatggtt tgcttcaaca cccgtaacag
    12241 attccgcctg tttagcaata aaaaggctta tgctccccac accacagtac atatcaagca
    12301 cattgccata gacttgtttt gcaatttccc tgtacattac ttgtgttata gatgtatttg
    12361 cctgaaagaa tgaattgggt gttattcgat agatgatacc atccagcgac tcttcaatat
    12421 atcccttctt tatatcctca aacacttcac cgtaacttac atctgccatc ccatcactca
    12481 aaataagcga aatggaatca acatcattat atatagcatc aatcactgat tcaagattgt
    12541 tctcctgccg tgccactaca aaatttacca tgacctgccc tgtattaaat ccctgccgca
    12601 ataccacata gcgcaggtac cctttgtgaa ccagataatc ataatcctgt acacctgaaa
    12661 gtttttcacg tattatggca aatatacgcc tgcttttatc ctgtaaaaga agacaatcgt
    12721 gcagatcaac tacatgttta aagcttcccc ttcttcgcaa accaattttt ccaaaagctg
    12781 taacataatc catgcgattt ctatatccaa ggggtataga aggttctatg gcatcaatag
    12841 tagctacagt attcagcagg gtattacaat actctttctt tatttcaagc tgtgtactgt
    12901 aatcaacatg ctgcagtgca cagccaccac agttgcctgc atacatgcat ggtggctcaa
    12961 tgcgtttatc agcaaacctg tcagcaatgg acaataaaac ctttcgatgt tttttcaagg
    13021 gctattactc caatccagaa acctgtatag attcaggata ttccacagca aagtgtattg
    13081 ttaattcttt tttctgattg ggggaaagct gtacctgcca tgtagctttt ccttcgttat
    13141 caatggtagc accaatactg tttttgttga taagggtaac cgttatcttt tcattttgcg
    13201 aaacaggaat tgcatcctgg acggtaacag taacatcacg ctttttccca ttggcaagtt
    13261 ttattgaata tacatattca atgcgttttg ttttgcttac cagcccttta tattcggtct
    13321 ttttacttaa aagttttctt tcgaccataa ggctttcatc aacacccagt gccactggta
    13381 ttaaatcttt tggtacgtag agtttattaa cattgaacga attgacaaat ttgccgtcca
    13441 gatatacatt catgttccca ccctgcatgg ggaaatcaaa aggattttgg aattcacctt
    13501 caagcataac atacggaagt actctgggaa cagttgcata gcgcactgca caatccgttg
    13561 ttgcctttgc cacaggtatt ttatatgcat taccatcgga tggtattgtt tttgcaccct
    13621 ttatttgaaa tgaaaatgca atacgctctt cctgcattgc aggcatttct tcagcttcct
    13681 gcggtgcttc agcagcgtcc tcacttctga gtttcatgct tttggccgat tccttataat
    13741 aatacgatgg ctggtaaaca tctaaatacc atgggttaag cacaggcttc tgggttgcaa
    13801 caaacggctt tgaggtcgat agttctaaag aaacatcctt ccagtcttca ccagtccttt
    13861 gatacacacg ggcattacat accacttcaa ctgtttgtgt ttcagtattt gtacgaactt
    13921 catattccat attccatcct gcattccctg taatatactg aatcatcact gttgcagtat
    13981 cctgaccagt atgcaccaca acaagctgta atgattttgt agcttcatct gtattaagaa
    14041 gtgacagctc tttttccagg cgctcctttt cttctttatg cttttgcagt gcctgctgca
    14101 acgtggctat ctttttatag cactggctac tatgttgttc aattaattgc agtatcgatt
    14161 gcatctgcgc tgctgtcatg ttaaccgcaa aaggcgatgt gctacctttt tttatatact
    14221 caagaacggt gttcaatgcc tgtatatcat tttgttttgc attaatctcg ttattgattg
    14281 attcaagctt ttctttgagt gcgttaatgc gctgcacatc gcctttttta agataggttg
    14341 tctgcacaga aacatcacgc aacaaagcac cttttgtaac cacctgaact gattcattaa
    14401 ccattgatgc aggcagtccc tctatggtaa tcacattttc acctttatgc aagtttacct
    14461 tagcttcttt tttaaccagt gcactgtcat aatatacaat aacgttactg atttcaggct
    14521 taactatttg tgcataaact acagtaatgc ttgtaataag tatcgccaat gtaaatattg
    14581 tttttttcat acagtttttc atacagaacc ttccttttac atggaatata gtaataaatg
    14641 gtattgtctt taatcttcac ataacagcct caatagccac actgtgcttt ttgaaatgtc
    14701 acaggcaaca gtgtttcctt agattccata aagaatgaac taccaaggca gtaaactgta
    14761 accgataact atcgccatta tataatacca tgtacgatag ctcatagtaa cggcttttgc
    14821 cattaaaaat ttatttctga atacgtacct gtgctatctg ccgcgatatc aatactaacc
    14881 tattctcatc ccaggtttcg ccatccgttt caagaagacc acaggttata aatcgggtgc
    14941 gcgtgtaaaa ccgcaaccat ttagattgtg gcatattcct gatattgatt gtcaattcaa
    15001 tagttggtac ccaggcaatc actcctaaac ttacaaacac agcaggtggt ataacatctg
    15061 acattaacag caaagcaggt atgtcatata ctctttcctg cctgaatgaa atccatcctt
    15121 tttgtataga ctcatcacgc agtttcccct gcatccatcc tgctgaatat ggatcaagcc
    15181 tgatatcaac atgtgaagat aatgtaaaat gtggtacttc aggaataggg acgcattcct
    15241 gtaacggtgc tacctgggga actggcgttt catatttttc aatatcacat tcaatctttt
    15301 catttgcaaa ggtgccccag catcgtattt tttctttgcc ttcctgataa aggcttgcct
    15361 gataccgatt aaactgtgca gactgcgcaa aacattcaac gtgaatttcg caatcacctg
    15421 gtttacatcg ggatatataa tttgctgtta ctatgggcgt tgatcgtttg tcactttgca
    15481 tagccattgc atttcccaac aatgcaatga gatagcctcc attggggatc ccacccacaa
    15541 gccagttttg tgaaatagta cctttgaatt ttccaggact tacctcaaca aggctgatat
    15601 cattatccag aacatgcata gactacctcg ggcaacacgt tttgtgtaag aataacctat
    15661 gcatataagg aattcaaaca ctttatatgt ttttttactt ttttaagaaa tgactaatgt
    15721 ttcatgaact ttaaaccagt tatcaatata atcaatagat acaattataa ttatatttga
    15781 caattttaaa gcatcacaag tactccaagc acactttgct ctggaaacgt gtaaacttat
    15841 cgtattgcca taagagcaag ggatatattt tacgtataat aacttgatat tatgaagaaa
    15901 aaattaccat cattttttga ttgcattgcg ctcttaacca ttaccataat tcttttattt
    15961 acgggaacag tactattcaa aataccaatc ccgctactgc ttatcattgc tgcaacgctc
    16021 gcaggcataa aaagctattt tttaggtcac aactggtcag aaattgaggg cagtattgtc
    16081 aatggtataa aaggcacatt acctgccatc atgattcttt tgactgttgg aatattatca
    16141 ggcagtttta tagcatcagg aactattcct ctgcttatag attatggttt gaagcttatt
    16201 catccatcat ggattcttcc cgcagcatac attgtgtgct gtataagcag ccttgtcatt
    16261 ggaagttcct ggactactgc agctactatt ggtgtggcgc ttgttgggat ggctggtgcc
    16321 atgcacatat cccctgccat ctgtgctggt gctgttgttt caggcgccta ttttggcgat
    16381 aagctttcgc cgttatccga tacaacaaat ttagcaccag gagtttctga aactgacctg
    16441 ttttcacata ttcgggcaat gatggcaacc actatcccgg catggtcact ggcattaact
    16501 ggttttattg tgttaaacat atacattgtt ccggatacta ctacccgtat agaacacatt
    16561 gaatcattat tacaaaccct ggaatcatca tttaacctgc acccggtcac catactcccg
    16621 ctggcagtag tgttcattat ggcactagtg cgttttccgg ctgttgttat cttactatca
    16681 ggaagtacag cagcaatact ggtgggatgg ctggcacaat cacaaagctt actttcggta
    16741 cttcagtatg catattcagg gtataccata cataccaata atgaaatcat taacaacctt
    16801 ttgaaccgtg gcggtatagt aagcttttta gagacaacgc ttattattct atgcgctaca
    16861 ggtttaggtg gcatatggga aagcgggggt tacataacat cgctacacga caatctgctt
    16921 cataaaatta aaaccagcag aatgctcatc atttcaacgg ttattaccgc tgttatttct
    16981 aatatcatta tggctgaaca atatttatcc attgttatga caggtagaat ttataaagac
    17041 gcatacaagc ggaaaaaact tcagcctttt atgctttccc gttcgctgga agatggtgga
    17101 acggtcacgt ccccactggt accgtggaat tcatgcggag catttatgag ttctgcgctg
    17161 ggtgtgccta cactgcacta tcttcccttt gcgtttttta atctggtgat gccggttgtt
    17221 gcaattgtgt tgacagcttt tggcctcttt atttacagaa agaatgatta gaataataat
    17281 tttttacagt gagattgtac tatgcaaaaa aatgcaaagc agatacctgc acgcaatgaa
    17341 ataccagctg aacatacatg ggatttaatg ccattgtttg tgtccgatag taactgggaa
    17401 gaagtctaca gtaacacaga acaattaatt cccagatata aaaactatga agggaagctg
    17461 gcagattcct ttgatgtatt tttacaggca attgaatttg acctttccat agcacgtgat
    17521 attgacaggc tctatacata cgctcatcta agaagcgatg aggacaccac caaccagaat
    17581 gcacaggcaa tgctgcagaa agctcactcg ttagcccagc gtgcagctga agcctcaagc
    17641 tttttagtac ctgaaattca atcactgcct gaggctgtta tcagtaacta tctgacaaac
    17701 actaacattt ctcagtatct cttttatata gaaaagattc ttcgctacag ggaacacaca
    17761 ttatccaaag aaattgaaga aattttagcc atggctcatg aggttgtcat tgcaccaatg
    17821 gaatttttcc gaaaacttga caatgccgac atgcgctttg gtacaattac caatgaacag
    17881 ggtaataccg ttgagctaag ccatgggaac tttataacat ttttaatgag ctataatcag
    17941 gatgttagga agaatgcttt tcacacctat tataaagcct acgacaatca caaatacagc
    18001 attgcacagt cactggccag ctctgtaaag aaagatgtat ttttttcacg tgtgcgaaag
    18061 tttaaaagct gcctgcatca ggcactgttt cctgataata tacctgacac tgtgtaccat
    18121 acattgattg aaacagtatc ttctaacctg caaccactgt ttaaatatct ttcatatcgt
    18181 aaagaggcat tggggcttac tgaattgcac ttctatgata cctacgttcc tcttgttgcc
    18241 gatgtgccat ttagcatgga atatgaagaa gcctgtgatg tatgcgcgca ggccctgcac
    18301 ttacttggtg aagaatacat ttctacgttg catacggggc tccttggtgg ctgggttgac
    18361 cgctatgaaa acagaggaaa ggttagcggt gcttactcat caggctgtta tgattcacca
    18421 ccgtatattc ttttaaacta tgatgccaat accataaata gcctgtacac actgatacat
    18481 gaagcaggac actccatgca ttcatactat tcacggaaaa atcagcctta cgtatatcat
    18541 gagtacacta tatttgttgc agaggttgca tcaacactca atgaaattct tttaagccat
    18601 tatcttttgc agcagtacag tgataatccg cgaatgcagg cttacattct taaccgtgag
    18661 atagacgaaa tccgtggcac actattcagg caaaccatgt ttgccgagtt tgagctactg
    18721 gtgcatacca tggcggaaaa caacaaacca ttgacatttc aaacaatgac tgatacatac
    18781 acaaacctgt tacacaagta ttttggtacc accattgtta ttgataccga tctatacctg
    18841 gaatacctgc gtattccaca tttttattca gcgttttacg tgtacaaata tgcaacagga
    18901 attgctgccg cactttcatt agccaaacag attataaaca atcccgatgc atcaaagcgt
    18961 tatcttcaat tcctgtcatt aggtggcagc aaattccctt tagaagaact tcgcattgcg
    19021 ggtgtggata tggaatcacc agagcctgtt attgatgcgt tacagtattt tgacagtctt
    19081 gtagacaggt ttattgatgt tcacaaaaaa attcattaat gcttcagctt ctgtaacagc
    19141 ttttgcgcct tgttattgcc aggatctgct tcaagtattt tgttacagta ctctatggcg
    19201 tcactggttt tatgtaagga agcacaaata gcagccataa cataaagcgc tggaatgtat
    19261 gatggccgta aggcaagcga gtgggttgca gctttatatg cttcttcata attgtgtaac
    19321 ttatactgag ctatagccaa caaatattga gccatgctgt catcaggttt ttttgccaca
    19381 tactcagtta agtgaaccac agcattactg tagcggcgtg tgtaaatata tgaacgggct
    19441 aatagcaata atgaggtcat atcttctgga ttcataatga ccccacgctt cagttcatct
    19501 attgcaagag tgtatttctt ttgggcgtaa agctcctgcc ctttttcacg atgctgcagt
    19561 gcactatgca tgatgggatc aagttcaagc agcacaaatg tcatgtcatc ccgtggtggt
    19621 gtgccaccca aaaatttttc ccactgctca atgatgtact cctttgcatc atcaatgggc
    19681 atatcaagag tttccagaag aagtttttca acccgttcat cgccaaactc ttcattgttc
    19741 cagttacgtg attccggaag cccatcagta tacaaaaata ccctgtcacc aggttccagt
    19801 atcccaaaat tatcctcaaa aagcccgcca atctcttcat tgggaagagc acctaaaaat
    19861 attccaccgg tatcccagta ttccagacga ccgctattgc gtttacacac aagcgcttta
    19921 cggtgggaac catttgcata catgacgtca tacgtgggaa atattaccat gaaaaatact
    19981 gttgcgtatt cctgtgtgcg gataatattg acaagctgat tattgacatc agcaaatatg
    20041 cgtcgtggaa gcaagtattt acggcatgat tcatggaagc taattttaac catggtgctg
    20101 ataagtgcag ccggaatgcc atgaccggat acatcggcaa ttaaaacccc taagcagtcg
    20161 ttgggcatgc gtatgatatc atacaaatca ccgccaatat cactggaagc acggttgtat
    20221 gcaagaacct ttattgcatt aaacacaacc ggtgtttgcg gcaatatccc ctgctgaatg
    20281 ctggcagcta tttttaactc ttttgcaatt aaatcatttt tttccttcaa tgccgtcagg
    20341 gcctgattca attcttttgt ccgctcttca accttttgtt ccagatttct acgtatgtca
    20401 ttaacctcat acgcagcagc taaaaagcct ttgctggtgt tgataagttc tttatctaga
    20461 tagggaagaa acaatgaacc gctgccaccc cgcgcaagat ctattgcagc agactcaatt
    20521 tcactcaatg attcttcaat tgacagcagg tagaaaaaca aaataacaaa tacaataaat
    20581 cctgtgataa caataaacaa aatgggtatt ggtgaaaata tgtcataatt attatagtat
    20641 atcataagtc ctatctcaag cattgccacc aggaccatga tattaatgaa tataaatttt
    20701 ccccgtatac ttaagatata gcgttcatta aaagaaatgc cacgctttac caaaagccac
    20761 tttaccttac ggcgcatcct tcccgataaa agctcggcaa caatataggt taaaaataca
    20821 tagatgaata ttgccgaaga aataccaagc aaaagaaata atgcattagt aaaagcccct
    20881 tttatggctt catacacaca tactcctgcc atcacaattg aaggataaat taatgctgcc
    20941 gtcatattat cacgcggcaa atgttcaaga gcttccagca atcgtactgc ttcagtatca
    21001 tcaattgtct gatgaagcgt attatgtact atatacgtat taatgagaga aagccgtttt
    21061 gtaaaacagg gaactcctgc gttatacaat aatccaaaat gtatataata taagggagca
    21121 tagataaaaa gcgtcatacc tgaaaaaagg attacgaaat cctggcctgt attgctaaca
    21181 tcaaacctga cagtagcata caaaaaaagt gctatgtacg ctgcaataat aaagccaccc
    21241 aaacttgtag taaatgctat agcaaaggta taggtaatcc gttcgcttat aaaattaaat
    21301 atggcaaaaa aaggcttaat tgcccatgtt tcaaattttt gtatggatat catcatcaac
    21361 tatcctgata gtacaagtat tttagatatt tagtatattc ttgtaaattg tagtatatca
    21421 tactaaaaaa atcaattaaa aaaattttta tatttcacag tacttacctc catggcagcc
    21481 actatttgtt tttattgttt ataaaataat ttatttgatt ttactattga ctcagtaata
    21541 tttttagtga tatacttatg atattgaata tttttaaaaa aatctggtta tatttaaatg
    21601 cagcaaatct caaatcctaa atcatcaatt gcagtattag tggtagatga tgaagaatca
    21661 atcagaacca tattatcccg ctatcttgca ctgaaagggt atacagtact taccgctcct
    21721 gacggtaatc aggcaatgga aattcttcat tcacagccag ttgaccttgt ccttaccgat
    21781 ttaatgatgc ccaatgttaa cggccgggag cttcttcaga agatggcaga gcttttccct
    21841 gaaattccta aaatagtgtt gagtggctac ggcaccaatg aagacatgct tgtagctctg
    21901 aaaacaggag cgtatgactt tatccagaag ccgcttactg attttgaaat actggataaa
    21961 gccgttgaac gtgctgttga aagtaaactc ctcaaggaag agcgtgaccg ctacatacaa
    22021 cagttaacct atcttaacga catcattggg tttttaaata aaggcgtttc acttgagcaa
    22081 atctttgaca tcattgccag ccgtatgaaa aaagctatcc ctttcaatcg tttggcgata
    22141 gctcttatta atgatgcagg gctggtagaa accacactgg tttcttctga taaaccagta
    22201 ttaattaaac ctggtgcaac attcccctta gaagaatcat cactggtact ggtagcacaa
    22261 acaaaaaaat acgatattat cagtgatttg gaggaatata gtaaaaaaca caattctgaa
    22321 aaggccagac tactactgca ggaaggcatg caatcgacca tgacagtacc acttgtagtc
    22381 ggaggcaggc tcaaaggctt tttactgttt gcatcatgca acaaaaatgc atttgcaccg
    22441 gaacatgttc aatttgctga aacaataagt ggccacattg ttttcagcct gcagcggggc
    22501 gaactgttgc atcagcttga tatgtacaat aaaaaattag aagaaatagt taagatacgt
    22561 acccatcagt tattaaaaac acaaaaagcc acaatatttg cattgagcag tttagcagaa
    22621 cttcgcgacc ctgaaacagg ctatcactta gagcgcatcc gtgggtattg tatgttgctg
    22681 gcacaaattg ccaaatatac tggtggattt ttagaaatta acaaccagtt tttacgcgac
    22741 ctgtatgatt cttcaatttt acatgatatt ggcaaagttg gcatcccaga tagcatactg
    22801 ttgaaaccag gcaagctgac ccatgaagaa tttgaaataa ttaaaacaca cactactatt
    22861 ggctataatt cgttaaaacg ggcttctgaa gaaatggggc gcgattcatt tctcaatatg
    22921 gcaatggacg taacattgta ccatcatgaa gcatgggatg gttcagggta tccccacgca
    22981 ctatcgggag aagacatacc acttgctgcc cgcattgtaa ccattgctga tgtatacgat
    23041 gcccttacaa ctgtacgccc atataaggca gcctttgacc atcaaaaagc cattgccatc
    23101 atgaatgaag aatcacatcg ctacgatcct aaactctttg aattgtttat acaaaatgca
    23161 gacgattttg atgaaattcg caataagtat cagtaatgtc tttataggag aattgtatgc
    23221 aatcatatta tcactttggc agcgctatta ctccatcagt aaaaaaaata attatattta
    23281 catccgttat ttatattata cagcttattg ggaatatcat taccccggga cttattgaat
    23341 ccacatttgg tatacatcac attggtttta ttcatcagtt gaaactatgg caacctttaa
    23401 cctacatgtt tcttcatggt aatttcttcc atatattttt taacatgttt gccctgtgga
    23461 tgtttggcgg tgatttagaa gaactttttg gcagtaaaaa ttttaccaaa tattacctgt
    23521 acagtgggat aggtgcaggt ttatgtatag cacttatgaa ttattatatt tttgacagct
    23581 atggatatag ccccattacc ataggagcct ctggcgcaat atatgcacta cttcttgcat
    23641 atggcatgat atggccagaa agggaagtgc ttttatactt tctatttcct atcaagatga
    23701 aatatctgtt attaatattt ggagccatag aattctttgg cacattagct tcagcatcgg
    23761 gggcaggtgg gaacatcagc catattgggc atgtaggtgg acttatcacc ggatttatcc
    23821 tattaaaatc aaaattctgg tacaaacgtg tttccttaaa cccctttgat tcactgttca
    23881 ggaactatcg cttagcacgc cacaaaaaaa gaattgaaga gcgtataaaa gccaaagaga
    23941 tcattgacac actcttagat aaaattaccc gctatggtta tgggtcatta tctcctgaag
    24001 aaaaacgcca gttagaatgg gcccgcaaac acttctatcc cagcaataat gacatcatcc
    24061 attaagtatt ggcgatagta accggggata aattacttct tgacaacaag ctctgttgtg
    24121 acaccatcgt tgtattattt ttgccatgag gtgccccatg tactggaaca aggatattga
    24181 aacaataagc cgtaaagact tagaagcgtt gcaactcaaa aaattgcata aagcattgca
    24241 ggctgcaagt actacaccat tttataaaaa ggtttttgaa gaaaatcata tcaatattga
    24301 cgccataaag acacttgaac atattaaaga cctgccattt acctcaaaga atgatcttcg
    24361 tttgagctat cctgatggca tggtggctgt ccccaggaaa gaaatagttc gcatgcacgc
    24421 atcatcggga actactggca aatcaacagt aatattctat actgcaaagg acataaaaga
    24481 ttgggctgac ttagttgcac gatgcatggt tgcaaccggt gcaacacctg acgatgtatt
    24541 ccagaatatg atgggatatg ggctttttac cggtgggctg ggcttacatt atggtgcaga
    24601 gctgttaggt tgcatggtca tcccagcagc ttcgggaaat acgctgcgcc agattcagct
    24661 gatgcaggat tttgaaacaa ctatcatcca tattacccct agctattgcc tgcacctggt
    24721 ggaagtaatc cgggctgaag gtattgatcc gtatgcaatg accgtcaaaa aagcatacct
    24781 tggtgctgag ccctattcag aagcaacccg aaaaaagatc gaagagcagt ttggcattga
    24841 tgcgtataat tcatatggct taagtgaaat gaatggcccg ggtgttgcat ttgagtgcac
    24901 ctataaaaat ggtatgcaca tctgggagga ttactatatt gttgagataa ttaatcctga
    24961 tactggcgaa gtactcccac ccggcgaaga aggggaactg gtacttacgc acatcaatcg
    25021 tgaagcaatg cccatcatcc gctatcgcac gcgagatctt acccgtctta tacctgagcc
    25081 atgcccatgt ggacgcaccc attaccgcat tgaccgcatt aaaggacgta ctgatgatat
    25141 gctgatagtg agcggtgtaa acgtattccc atcacagatt gaaacggtac tcatgaaatt
    25201 tcctgaggtt ggcaacaact atcaaataat cctggacaga gaaaataacc tggacaggat
    25261 gcatattaaa gttgagctgt attcaaagct tttccatggc gatattaaag aaattgaacg
    25321 gattaaaaac aggcttaaag aggcattgaa agcactgatc accattaatc cccgcattga
    25381 gcttttagaa ccgggttcgc tgccaccaag cgaggggaaa gcaaaacgtg ttattgataa
    25441 ccgtacgcta taatactgta aggggaggta tcatatggca ttccaggtat cagtatttgc
    25501 tgagaataaa cccggcaaaa ttgaaagaat aacaagtata ctggcaaagc acaatattaa
    25561 tatcagggct atcaccataa atgattcagg tgattatggt attatcaagc tgctattgga
    25621 caggcccgaa gatggatgca ctgctctgaa agaagaaggt gttgccgcca cgttgaaaga
    25681 gatagtagct gttaaaatag gcgataaacc cggaagcctt tataaagtag cagccattct
    25741 caaagaccat gcaatcaatg ttgatgatgc ctatggcttt acagttaagc ctcatgaaga
    25801 atctgtattt gtattccagg ttgataatcc aaaagcagca gaaaaagccc ttaaagatgc
    25861 aggctatcaa ttattgactg acaaggaatt atatttgttg tagacagtat tttctttcag
    25921 gtagcaagat tcttcagtcg tcgcggtgaa accagggata cttcaacaat tatatttata
    25981 tgaatttcta ttcttaccat ttttattttg gaggaatata tatgaaacaa tatatttcta
    26041 tattgtttat tagctttgcc tttatcctgc tttcatgttc acagcagatc agcatacagg
    26101 gaaagccgga tgtactgata aaaaaatacc ttaaagatga ctcaacctat gtcattatgt
    26161 gtaagggata tccaaagccc aatacaacag gcattcaggc aactgaaaca gccaaagaag
    26221 cagcactact caatgcccag tatattgccc gtggtatttt ttctgaagaa gttgatgttg
    26281 tcaaagatgg tattgtagat ggcttttcaa tcaatccaga ttatgtagtt atcacatatt
    26341 caatacgtca tcccaatatc aagcgctata tacgggatga ataatagtat attgattgca
    26401 ttattttttc ctgaatgtaa acagtgaact aatataaaaa cgtctatact cctatcacat
    26461 gcaagtcgtc atgggcaaat attctatttt taagtaattg ttctctatac agctgattcc
    26521 attatttcat ttcattcttg ctgatatagt gatgataaat catatcttaa atatttttca
    26581 atttttcctc tctttgatca tctcatggcg catcacatca tagttgctca ccctccatag
    26641 agtacctgat acccttacga ttaggaatca tgtgctcttt gattataaag ttatggaaat
    26701 cacgtatgaa aacaaaaaga ggggcattgc aatgcccctc tttttgcaag caagaacatt
    26761 gtaattactt ctgagctgga gcctcttctg ctggctgttc ctgaactggc tgatcagtag
    26821 caggagctgg agcttcttct ttcttgcagc caacaaatgc tacagcaact gcgaaaacag
    26881 caataagaat aagagatgcg aatttcttca tggcgttact ccttttataa tattaaagat
    26941 ttattgtgtt tgagtatggc tacaccaata aacacagcca tataaaaaaa tcaagtatat
    27001 attaaaaaaa ttttttctgg taagttgaac ggcatgttgc ggattcccat accgtcacgc
    27061 tgagtacatt aatgtttgtt gaatgatttt gagaatattg ttttaacatt ttttcaattt
    27121 tttgatatat aattttagat aacaattctg aactggggtt ctggtgagca aactcctgta
    27181 tatcatttaa atttttgtga tcaaactcct gcaatactgt tttaagcata tctttcagat
    27241 catggaaatc aattaatatt cctatatcat taagcttgtg cccatacacc gatacttcta
    27301 ctttccagtt gtggccatga atattctcac atttcccctt gtaccctttt aactgatggg
    27361 ctgctgaaat ataatcttct attgttaaaa tgtacattcc tgtgctcctg attaatcgtt
    27421 tatgcaaaca tggtcaacac cctgcttact ttttctattt cacgtttggt aagtgtgggg
    27481 tagatgggca acgaaaaaag tttttttgac aacctgtcac tgtgtggata atccagattc
    27541 ctgagtttac agtactgatg caaaggaact tcaacaggat ggaatatctc aatgccattc
    27601 ttcttaaaaa atttttctgc tatatcaggt tgtgcatcaa agagtaccgg aaaagcctga
    27661 tatgtgcagt gatcgttata tgcatatact gtggtgtgat gtgttttgtt caacgcttca
    27721 tagtatatgc gggcaatatc acgtcttcgc ctgacaaaat ctttcagttt aagcaactgg
    27781 tttaagccaa gagcagcatg aaggtcgctc atgcaatatt caaacccggg aatatgagaa
    27841 tgcggcacac catttcgaac atcacgtatg tatgaataat ggcgcgaatt attggtaaat
    27901 accattgcgc ccatttctgt tgtaatcatc atcgatggcg aaaatgatgc aattgcaatc
    27961 gatggtgatg gcaggaagat gtcagcagtg tccataccta tggcatgtga gatatcaagg
    28021 actactggaa cggaactatc gcccaaatca aaaccaagtg gtatgccaaa catgtggccc
    28081 aacacaattg ctttagtttt ttgtgtaata gccttctgga ttaattccgg tgaaggatga
    28141 taagcacctt cagcaatatc aattaatacc ggtgttgctc caaccagatg gcatgccttt
    28201 aatggcgctt catgaaaaaa agatggcatt atgatctcat caccccctgt aacacccagc
    28261 gccataaagg cacagtgata tgccgcagta actgaattgg ttgccagagc atacttcatt
    28321 cccaatagat ttcccagtgc ctgttcaaat tgcttcacaa cagcaccgga tgccagagta
    28381 tcctgaataa gacaatcaag tacaccttcc agttcctttc gtgtaatagt tggtttgctt
    28441 gaaactattt tcatggttct ctattgccta ctatataata ttcaaatgaa acgtacatgt
    28501 gcgtattatt aaaaattggt caagcatcat ttggcaagaa taaaaattaa atcaagcaac
    28561 ggttacgtta ttccgatact acacatagta taggtattat caaaggaggc attcaatgcg
    28621 taaattttta ttagtacagg tggcgatagt tgttgtagca gcttttgcta tttcatgtgc
    28681 acctaataat gtcatgacca aacgtgacga aacatataca tttgaccaga tgagtgtaca
    28741 ggatgagacc cctgtatatt ctgatatgga accaactcaa atatcatcaa atagtagcga
    28801 tagctcatat tatcaggtag gcatagccag ctggtatgga cgagagttcc agggaaggct
    28861 caccgcatct ggagaaccat ttgatatgaa taaatatact gcagcacacc gcacacttcc
    28921 atttggcagt gttatactta tcaccaatct tgaaaatggt aaaaaggtaa aatgtactgt
    28981 taatgacaga ggaccattca agaaaaaccg cgttcttgat gtatcatata aggctgcaaa
    29041 ggatctggat atgcttacta ctggcgaagc taaagtgggc attacagttc tttcatacgg
    29101 caacaataca cagccaggaa caaagtcaca tgatgttgga gtatttggcc agcctgttga
    29161 taatgacaat gataactcaa acaatgcagg catggagtca aaacaattca ctggactggc
    29221 attgcaaaca ggtgcttttt attcaaaagc taatgcgtta aaacttaaag aaaagattga
    29281 aggacttatt gacaagcccg ttactgtagt acaggaaggt tattattata aagtccgcat
    29341 caccaatatg aatgaaaatg aagtttcacg ttatattaaa gttttgaaaa aaaatgacat
    29401 tccttcatat aaagtaacaa ttaatgaaag catgaatgat tagatacata cggggctgtc
    29461 ccagaagtcg tttcgataca aatttcgcaa cgctcattga gtaattttgc cagatattgc
    29521 ggcaaaattg tatcgaaatg agcgagtatt acgatataaa gtttgcaaaa tttactcaac
    29581 gacctctttt gggacagccc cttgtttttt aaaaaagctg atgtagtaac gttacagctt
    29641 taacaataaa aaagtactta tccacagaaa gaccttaaat tttcctctac acattatctc
    29701 ctgtatgatt ttttagcatt gtcaatgtat tcagaataat tattgatttt tacagcatat
    29761 attccgactg gtatacattt atgattggta catcccatag ctagtagcat ttaaccaaat
    29821 atgtatgcac tatggcaatc gtgcgccagt agattactgt attaatgcat atactatgaa
    29881 cagtaaacga atagttcaca tcgattatct gaaagcatgg gcagtaattg ttatgataga
    29941 agtccatgtt gttaattgtt tcctcaatcc atcattcaaa actgcatggt ggttcaatat
    30001 agtaaatttc ataaacggac ttgtggctcc agtttttatc tttactgcag gtttttcatt
    30061 tgtcttttca gtaatgaaaa agcgagaaga acttatcagt ttatcagatt tattttttag
    30121 aagaacaggc agaatactat taatagttct tgtgggctat cttatccata tcccctactt
    30181 ctcattaaaa aatattatta tttatgcttc acctgctgaa cttaaatcct tttattcagc
    30241 agatgttctc caatgcattg gatcaggttt gctggtgtta ctatttctac gaatggtaat
    30301 tccgtcagat gtgctgtacc atagtatcat caccggcatc ggtttgttga ttgtttttat
    30361 aacaccctat atatgggaga ttgacttcta ccaatggttc ccattgccaa ttgctacata
    30421 ttttaatgaa atgcatggat cattattccc agtatttcca tggcatggat ttttgttttt
    30481 aggcgcagca tatggggttt ttcaattgca ccataacact aatatccatg cagacaaatc
    30541 tttaaataca tttgcagcaa tttcttttat tattagcatt gctggcatta tttctatgca
    30601 tttcttatcc caaaaccccg catataccat aaagacaagt gcaatctttt ttataacacg
    30661 gtatgcactc gtcatagtgc tgctgcattg ctgccagtgg tatcttcata taaaaaataa
    30721 tcagggcgga ttattcatca atataggtaa agaatcactg cttgtttact ggttacatct
    30781 tcagatatta tacaggcata ttttcaatgg gaaatcactt gaaagaattg ttgctcagaa
    30841 atttagcttt gcagagtgtt tgacagcttt tcttttgctg gcagccttga tgtttgcagt
    30901 agcaattatg tggaatacat taaaacaaca ttatcccgat atttcccgaa aaatatttat
    30961 tggaacaata ctaattggat taataaaatt tttatctttt taatcttaaa ctgcttttgt
    31021 ggcctttaca aggtactgta acgcagtttg attgttcccc acaaataaat ggcacagact
    31081 taacgatatg aatgcctgtg gtgtaggctc tttctgtatt tcctgctcta attgctgtat
    31141 cttggtatcg atagtcccct tgctgtcatt gaaaatggtg tgaggtattg ttgaaatata
    31201 ttcattaaat tgcctgaatc cctttttctg caatgaatgg gatataagct tttcatcatt
    31261 ataccgtgca cactgtaaaa ttatatctga agtaatttcc ctgaacctga atgagtcatg
    31321 gtacctatca tattgctgta aatagcgtaa ggcatcggta taattttttt gttcataata
    31381 taaaagtata attgcggtca gggcaacttt atttgcaggg tccttcttaa taacttcctt
    31441 aaaacatcca agagcagctt tataattatt aattttgctg aaagctatac ctttaaccag
    31501 gagagcaata taaaaatctg ggttcgatgt actacactta ttcaggataa taatggcttt
    31561 tttatattca cccaatctaa aataaaggct tccaatgttg aataaaacaa tataatcttc
    31621 cttcagttgc agtgcacgta aaaaacaatc aagagcatga ttgaattgtt tcatgcgata
    31681 atacaatgaa cctaaattga ttaatgcagc cctgaaagaa ttatcaattt ggagtatctg
    31741 tatatataat tgcaatgctt cagtaacatt accctgctct tccagccgaa gtgcttctat
    31801 aaattgttcc tgtatagtca ttcaatcaac ctttccaaac aaataggcat tctattgctt
    31861 actatcggca ttttatttgg caagcgcaac tttatttcgt cccatctctt ttgccttata
    31921 taatgcttta tcagatctgc ctacaaattc ctttatagaa gtatctcttg caggatcata
    31981 ctgagtaact ccaatgctta tagtaacatg caacgtactg tcctgcccct tgaatggatg
    32041 ctgttcaaca atcttacgca agcgttgtgc cacaatacct gcctgatcca gtcgtgtctg
    32101 gggcaatatc accgcaaact cttcgcctcc gtatcggcag ggtatatcca ttttacgaag
    32161 gtctttcaga aatattgcag caacttcttt cagtacacta tcgccctgca aatgcccata
    32221 ggtatcgttg aaccgcttaa aatggtctat gtcacacatc aacagtgaaa ggggtgcatt
    32281 atatcgctga cttcgtttca tttcctcaaa caatcgtatt tcaaaataat ggtgaatata
    32341 caaccgtgtc attctatcca gtgttgacat taaatacagc cgtgaatttt caactgccac
    32401 cgcgccaaac cgtgctacat aatgtaaaaa ttccttttct gaatttgaaa aaccaccacc
    32461 ggttattttt tcccctaaaa gtagaacacc catcagtgaa ttttttgatt tcaaaggtac
    32521 tactatgtat ggatttaaaa acgctatcaa tttgaggtca tcagccaggt cgggtaactc
    32581 caggagtgtt tcatattcaa ttgggtatgg attattttct aaaaatccaa cgagtggact
    32641 tgtttcctgc aatatcacat tttcctcaaa atcactgtca taccctttgg ccatatgtat
    32701 ataaaaatca ttcacatcaa tgtctttttg caacagcagc gctacctgtt caacaaacat
    32761 ctggccaata cagctgtata aaagggattc aaccaggctt tcaaactgca aatttgatga
    32821 aagacttatt cctaactcaa gcaggttgcg caggtcataa atcttttttt ccaggccttc
    32881 tatgccttcc ggtgttggtt cagtttcatc aaaaaaagtt aacccattat catccataca
    32941 taccctgtaa aataattctt gcgtttttat tacacttaac tgtttatgac gttatatgct
    33001 gtatcatcaa tatatataca tgtaaaaatg catagcagat ttttatacta tttacaaaaa
    33061 atgtcaataa ttatatacta attgtatcag ttatttaata aattgttatt attaccatgg
    33121 agacaataaa agtaaaatta caaccgggcg ctaaattacc cgattatcaa accatacatt
    33181 catcaggtgc tgacctttat gcattactgg atacaccggt caccttgcag ccactgcagc
    33241 gtgcgctcat acccactggc atctctattg aacttccccc tggctatgaa gcacaaataa
    33301 gaccacgcag cggtcttgcc attaatcacg ggattactct tcttaatact cccggcacca
    33361 ttgatgctga ttatcgtgga gaaattaaag taattgtgat aaacttaggt gataaaccat
    33421 tcacaattga aaacaatatg cggattgctc agatggtcat tgcgcatact ttgcaggtaa
    33481 catttgaagt ggttacagaa ttaaccccca caaaacggaa tgacggaggc tttggacata
    33541 caggcttatg aaagaaaaga aaatacgaat tataccaata ggtggccttc atgctattgg
    33601 cggtaacatg atggccattg aatatgacaa tgaaatgatc ataatagact gcggcattac
    33661 cttccccaat ggtgaaacac ctggtattga ttttatgata ccggatttca gctttatact
    33721 tgagaataaa actaaagtta aaggaatcat cattacccat ggccatgaag atcatattgg
    33781 ggcaataccc tttttactgc aaaagataaa tgtaccggta tatgccacaa aactaaccct
    33841 tgggctggta acaagcagac ttgccgaacg gccggtaaat gcacctacat tcattgagat
    33901 agagccacgt gatgtaatac atattggttc atttgtcatt gaatttttac gtgtgaatca
    33961 ttcaatcatt gatggcgttg ggcttgccat aaaaactccc attggcacta tcatacacac
    34021 tggcgactat aaaattgact actcgcctat tgatggtaat gttgctgacc ttcaccgctt
    34081 tgctgaatat ggtcagcagg gcgtgctgct cctcatcagt gacagcacca atgcaaccaa
    34141 tcccggttat acaccatctg aaatagtgct gaaaaagaag ctccttgaaa tatttgcaaa
    34201 gtcaaaggga cgtatttttg tcgcaagctt tgcttctaac atacacagaa tccagcaggt
    34261 tctggaagct tcacagaaat ataatcgtaa agttgttatt tccggaacca ccatgcaaaa
    34321 aaatattgag atagcaactg aactaggata cctgaaatac aaaaaagacc tgattataga
    34381 tgcaaaggat attggcaagt atacccataa aaaactggtg gttctgtgta ccggttctca
    34441 gggtgaaccc atgtcagctc tttcccgtat ggctaccggc acgcacaagc atttcaggat
    34501 aggagcaggg gataccgtta tcattaccgc ttcggttatt ccaggaaatg aacgcatggt
    34561 gtacagtgtt atcaattccc tgatgcagat gggggctgat gtattttatg aacaggatga
    34621 agaaattcat gtgtcagggc atgcgtcaca ggaagaactt aagcttatga ttactctgac
    34681 tcagccaaaa tactttttac cggtacatgg cgaataccgg catcttaaag cacatgcctc
    34741 actggcagaa tcactcaaca taaaaccaca gaatatcatt gttgcccaga atggcgatat
    34801 aatagagcta acaccaaacc gttttgaaaa gaaggggaat atacctctct ctaccgtata
    34861 tgttgatggt tcctatattg gcgatatcac tgatgacatc attgctgaac gtaaaacatt
    34921 ggcatctgat ggtgtcattg ttatcagcat tgttataggt gatgggttgc tactgtaccc
    34981 gccagttatt atagccaggg gttttattgg cataaagaat gatagattta tgcaggtgct
    35041 tcaaaaggaa ctatcgaaca ctattgaaaa gcagctttta cagaatgcct cacctgaaca
    35101 cctgacagtc gcagtaaaaa agcttgccaa aatgattgcc catcggttta ccaacaatac
    35161 gccactcatt gaaactatca ttcaggagat ttaacatggc ctacagtatt gttattttct
    35221 gcatcctggg gatcatccag ggtgtaactg aatttttgcc tgtatcatca tcagggcacc
    35281 ttgcactgtt agaggcaata ccacaggttc acactatctt tggtgcaatg gatacgggcg
    35341 ccaaactttt ttttaacgtt gcactgcacc tggcaagcct ggcagcaata atcatatttt
    35401 actataatga catcattaag cttgttaccg gaactatcgc cgaaattaca aaaaaacact
    35461 atggtacaca ttgccagttt gcctgcaata tcatagttgc ctcaatacct gctgcgatgg
    35521 ttggtttgtt tctcaacgac gttgttgaaa catcaatttc cagccctgca cttgttgcag
    35581 gtcttctgat aattaatggt atcatgctca tagtatctaa taaaataccc aaaaaaagcc
    35641 gaaaatgtga agagatggga atactgcagg cattgcttat tgggcttttc caggcagttg
    35701 caattttgcc gggcatatca cgctcaggta gcaccattgt tggaggtctt ttaataggat
    35761 taaagcctga agaagctgga aaattttctt ttttaatggc aataccggtt attgccgggg
    35821 caggactgct tgaattgcgg aaattgggca ccattaatgc ctttgaattt ggtattcccg
    35881 ttgtgattgc tatggctata acatttattg cagcacttgc ctcattgaag ctgcttgtgg
    35941 caatgctcaa gtcgctgcac ctgcactatt ttgggtacta taccattata gcgggggtgg
    36001 tagcaataat tattttactg gtataaaatt acaggtacag gattagcaaa cgtgtatgtc
    36061 caatcgtgtc actgaaatac tgtgcgttat actgtggggg tttgctgtta ttgtctttct
    36121 ttcactggca acattccaca tagctgatta tgatactctg ggcaccatgc cagtacaaaa
    36181 catacttgga cctgccggag tgatacttgc tcagatactc agaatatcgt ttggcatagc
    36241 atcatatttt gttgttgcaa tactgctttt aaccggatgg gggcttgtca aatataaatc
    36301 gttagaacct gtcctggaac atatctttgc cataaccttt gcaatggttt cggcttcact
    36361 tcttgtaacc attatacaac cggaaactat tgcacctttt gcaggtggag tggttggcat
    36421 ggcattatac cacgtgttta cttcagtaat gggtttgact ggtgcatgga tcgccacaat
    36481 acttttggta cttatttcac ttatactgct tggcatagtg tcattaagct atttctttga
    36541 tagccagcgg tttacctctt tagcaaacgt tgctgcgttg ctgtatagaa agattgtgct
    36601 ttttaagcac agggattcca tacctcttat tgacattaca tcattcaatg ccgaaaagaa
    36661 gataccctgg atcacaagga ccaaaattac cctgtataaa aaaacaccag tgcgcaaacc
    36721 aaagctcatt gaatatgata acatactcac gtataagggc gatagttcct attataataa
    36781 aaaagaccat acagatgacc tgcagataat caatacacat actacaccta ctgaaaatag
    36841 catacttgat aattcagatt cttcttccat gaataccgct gataccgaaa gcaaagaagc
    36901 tacctcacag gttgttgccg taaccaatgc ggtgattgaa ctgccacagg tagacagtat
    36961 acaggatgaa catgagccca cagtgattac aaaggaaatc agtgacattg atgaacatta
    37021 tgatgaagat gatgaatatg atgatgcaac agataatgaa gaagacatgg ttgcccgtaa
    37081 cgtttttgag gaaataccaa tcaatcagaa gtacagcata cctgtagact ttttaaagca
    37141 atacaccgtg catgatgaaa caggctataa acaggagata gcaaaaaatt ctgaacttct
    37201 ggtcaacaca ttgcgggact ttggcattga atcaaaagtt gttaatgtta accgtggacc
    37261 ggttattaca ttgtatgaat tgcagatagc tccgggtatt aaagtcaacc gtattgttgg
    37321 gcttgctgac gacattgcca tggcacttgc cgcttcccgt gttcgagtgg tagcaccaat
    37381 tcccggcaaa aaggccattg gcgtagagat acccaacaaa gtgcgagaga cagtaacatt
    37441 aaaagacatc atttcctctt ctgagtatac ccatcagcag gggcgcctga aagtaggtct
    37501 gggcaaggat atcatgggca aacctgtggt tattgatatt aaaaaactac cacacctgct
    37561 tattgctggt gcaacaggtg ccggtaaatc tgtatgtgtt cattccatca taacaagcct
    37621 ggtgtacaac tatgatccca actatgtgcg ctttatcatg gttgatccaa aacttgttga
    37681 actgcagata tacaatggca tcccacatct actgaccccg gtcattattg agccattcat
    37741 ggtaccaaaa gcgctgaagt ggaccatgat ggagatggaa cggcgctact acctcctttc
    37801 agaatataac acacgcgaca ttgaccgcta taaccagatg gttgaacgca acaagaatgg
    37861 cgaatacctg ccctatattg tcatcatcat ggatgagctt gccgacctca tgatggttgc
    37921 cgcaaaagaa atagaaggct acataacccg tattgcacag aaagcacgtg cagtgggcat
    37981 ccatctggta ctggcaaccc agcgcccatc ggtagatatc attaccggca tcatcaaggc
    38041 aaacttccct gcccgtattg catttcaggt tgctcagaaa actgactcac gtactattat
    38101 tgaccagaat ggggctgaga aacttttagg caaaggcgat atgctgtatc agtctccaat
    38161 gagttctttc ccggttcgga ttcagggcgc atttataacc gaggatgaac tgacttctat
    38221 agtttcatac cttaaaaaaa ttggcaggcc acggtatgtt gatattgaac aaagcctggt
    38281 ggagtttgaa gatgatgatg aagaaggtga agatgaactt ttcatagaag cagtaaagat
    38341 agttgaaacc acaaaaaaag cctcagcatc ataccttcag cgcaggcttt caattggcta
    38401 caaccgcgca gcacgtatca ttgagcgcat ggaagaatta ggatacattg gcccgcagca
    38461 gggaagtaaa ccgcgcgaag tgtatatata aaaaaattta agtgactttt tttatcccat
    38521 attttattat atctttatac tatccactat tgaaagtgat agcatgtgca tattattgga
    38581 tgtatttaca tgtatatgtg cttgcgatct atgcattaga ataattcttt tcagagatga
    38641 aaatattttt accattaccc ttaatagttc tttcttataa acagatagtt actgagcctt
    38701 tcttcaagtt cagagagagg tttttgaagg aaggggtgag tgaggtaaag atatattttt
    38761 ttacgaagta gttgcaagat gttcattaaa tcatatgtga ggcgctctat gaaatatacc
    38821 aaacaaacca cttttattgt cattattatc atggcaatag ctctttctgt cagtgcaata
    38881 cctggcaaga aagtaatagg tgttactgca tttaccaatc tgtcaaaaga tacaacgttt
    38941 gactggctgt gtattggtat tgctgatacc atatcctata aactgcgtaa cgtgcaggaa
    39001 tatatactgg tggatagggt caatgttgat aaactcattg gcgaattaca gctatcgcaa
    39061 tccggtctca ttgatgaaaa caccgttgaa gccggcaaag cattgggtgc caatattctt
    39121 gttgttggca atttccagaa atttggtgac cagatacgca ttacagcaaa aatagttgat
    39181 gtacaatcac acgccgtcat caatcaggtt caggttaccg gatcattgaa tgatatattc
    39241 aacctgcagg attcgctggc actgggaatc attgaaaaga atgatactgc cataaccacc
    39301 gaaacaaaaa caaaaattgt acagaacgat accaataata taagtgcata tgaatacttt
    39361 gttcgtgggc aaaagttttt actgtatcaa ttagattatt ccaaagctat tgaaatgtat
    39421 cagaaagcaa tatcaataga tcccaactat gcaatagcat acgcaggact ggggaaagcg
    39481 tattcactgc gttcatggga actcagagaa taccataata ccaccgaccc tacactaatt
    39541 gaaaaatcat tccagagcag taaaaaagca ttatcactta atccaaatct ggatgaagca
    39601 catttatcat tggcgcgcta ctatcaggaa gttgaccaaa ataaggtgcc caataaatgg
    39661 acattatgtg aagatgaaac gcgaaaggca cttgaaataa accccaataa cggcgaggca
    39721 tatctgctat taagcaaaat atatgcctat gatatcccca aatcagaaat gtatttaaag
    39781 caggcaattg aaaagaacaa gttcctggta gatgcacata ataacttagg gattatttat
    39841 ttagaccgtg gagatttgga cacagcgttt gcatacatca aaaatgccat cgatattgat
    39901 gaaaactatg tcattggcta catgaacctg ggtgttattt atgaaaaacg tggtcagtat
    39961 gataaggcac tggaaatgta taaaatggct gtttcaaaat accccaacta tattctgggg
    40021 ctgcgtaacc tgggaatagg ctaccgtaat ttgaatagac ttgatgaagc ccttgatgcg
    40081 ttttttaaag cattaaaact aaaaaaagac gatgcacaaa cattgagtga gattgggtat
    40141 gtgtatctgc taaaaaacga ttacaacaaa gctatcaatt atttcaaaca gtcgttacaa
    40201 tacgatcctt cgtaccgtta cagccttgct aatttagggt ggtgtttggt acagcagggc
    40261 aaatataatg atgcaatccc gtatttagag aaagcacata atgacaacag agattatgca
    40321 tgggcggcag gatatttggg ctggttatat gaaaacaaaa tccaggatta caataaggca
    40381 ttgtactggt atggcgaagc actaaagcgt gatcctaata atcaggacta tcgcaattca
    40441 tataaccgtt tatcaggtta ctatcgctaa actatacagt atcagtatac tgttatgaaa
    40501 gaagcattgt attatacaaa acacagtgag gatgcggtac agtgtgtgct gtgtcctcac
    40561 aactgtatca tcaaggaagg aaagagtggt atttgcaatg cccgacagaa taaagggggc
    40621 gtactgtatt cgttaattta tgaaaagccc tgtgctaccc atgttgaccc aatagaaaaa
    40681 aagcccctgt accatttttt gccgggaagc aggtcttttt cccttggcac tgtgggctgt
    40741 aatttacaat gcatgcactg ccagaactgg gaactgtcaa caagctcccc caacgacgtg
    40801 ttttgccata cgctcacagc agatgactgc gttaaactgg caattcagta tgaatgccag
    40861 tcaatatcat acacctataa tgaacccagt ataaacattg agtacgttct tgaaacagcc
    40921 atcaaagctc atgataaagg cattaagaat gtcatggtca ccaacggatt tatcaacgaa
    40981 gaacccttgc gacagttata taagcatatt gatgcagcaa acgttgacct gaaagctttt
    41041 gatgatgaat tttataaaaa aatatgcaag gcacggctgg aaccagtact gaaaacatta
    41101 accattctta aagaaatgaa tgtatggatt gaggttacaa acctggttat acctacacac
    41161 aacgacgatg taaccatgat tcaaaagctt gtggactggt ttgttcacaa tttaggtacc
    41221 gatgttccac ttcattttac agccttttac cctgcacaca aactgacaaa tgtcccccct
    41281 acaccactca gggtgctgaa acaggcgtat gacattgcat ataaagcagg tattaaattt
    41341 atatatttag gcaatgtccg cgaacgctcg gttacccgct gtccctcatg cggtcgtgat
    41401 cttattgttc gcagcggatt cagcgtgatg cgcaccaatc ttcatgatgg aaaatgtata
    41461 tgcggctact ctattcccgg agtgtggcga tagttactac gaatgagctt caacctggta
    41521 caattcatct ttaacgggca gtgctttccg tagattcttt atatacggat aaagatcccc
    41581 agcttcaaca taataatcac attcatccat tgtgcgccgt gcaacagtga aatatttatg
    41641 aactttttcc tcccctgagc gtttcaacca ctgcttcttg ttgcatcgaa cacgcaatac
    41701 aaatgttttg ttatcgcctt cataatgtct gaatactgtg cagtgcatac aattggcaca
    41761 aaagacctta ttttcttcca tgtataatct cctgttaggt atagtatctg tatctatgag
    41821 tacactacaa ccggtatgat gtagctatta tagcataatt aatgaaaaaa tcaagtagta
    41881 tgttgtggtt tatgacaatt gataccatac attatttttg caagcatttt ttagaggtaa
    41941 ccacaaatca tagatacttt tctactatat tcaattgcag caatctcaca ttgtggtgtt
    42001 tggtttacca atatatcttg atgggatcac agctcagctt aaaacagtta ttgacaggat
    42061 agtctgctgc atgaagccct ttttgtggac ggatgtcttt ggctttagcc gtcattcatt
    42121 ttctttggaa tttcctaaag atattatagc agtgagtacg tgtggatttc ctgaatatga
    42181 aacatttaca cctttgatta gttattttaa cgcattatca aaaaacctta attcccgtct
    42241 tgtaaccaca acgtttatct ggggttcaat agcaatacag atgaggatag agttactgga
    42301 tttaaaattg aaacttattg aatctgcagg ttatgaatgt tgttaaacga ttgtattgac
    42361 aataggctga ttgatgcaat acatcaaccc ctttttacta aggatgaata ttttaaaatt
    42421 gcacacactt atgaagagta gtacaggatg agattatata aataaaccaa taattgttgt
    42481 aaacttaatc agaaatatat aaactaaaaa actactgtgt agtagctgta ctatttaatt
    42541 caacgcaggc aaataaattt cgaaacgaga acccttacca ggtgtagatt caatattaat
    42601 atatccattc atctcctcaa taagatgaaa taccattgcc aatcctagac ctgtccccgt
    42661 atttgcatcc ttagtggtaa aaaatggctt gccaagattt tttatcgttt cttcatccat
    42721 tcctacacct gtatcagtaa taataactcc aaacattctt cccttatttt catcatattt
    42781 ttcaattata tcaatagata atatattatc atgtggactt ttttctttat ctctcataat
    42841 tgtcatagca tggatagcat taatacatac atttaacaat atctgcataa ttgatgtgct
    42901 atcaccacat ataaattttt tcttattatc catattgata gttatggtta ttttttttcc
    42961 ataagctgtt ttacatattt gagcaatttc atcaacagct gtatttaaat ctatttgcgt
    43021 gaatgagtta ttcttttttt gattcaatga tctcaatcga tttaatatat caaatgcact
    43081 atcaaaagag ttctttatta aaacaaaata tttttctaat tgagacgctt tatcgggagc
    43141 aagattgaca atttctttat gtaataatga cacaggaccc gttatcccca ttaaaatatt
    43201 attaaaatca tgaagcatgc cccgtgatag aatgcctaac acttctgctc tttgtgcctg
    43261 agctaaaagc tgctctgttt ttctacgagc cagaagttct ttttcaagct cttgggtttt
    43321 gatcataatt gtgtgtttca atgatctgtt tataaaagat ataactagaa taattattgc
    43381 tgctataatt attaaggtca ttattgctgt ataaaaccgc caatctttca ccatattata
    43441 tgattcttca acgagccatt tacgtttcat ttcaagtaaa tcatcctctg taattaatga
    43501 gatcccctca ttgactacat taagtaaatt tacattattt ttagggaccc ctgcatgaat
    43561 atcctcttcg gacaagatgc ttattatttt aaattcacca agaatatgat aatttgacag
    43621 ataatatcga gcacttggtt catcttcgga aaaagcatca atttctccat ttgctgccgc
    43681 ttcaattagc ctggcagtat cctcataaac tttaatatga atccatggaa atttttcttt
    43741 taagaataga tatgcaccgg tatcatattg tgctccaatt tttagtgaac tatcatgtaa
    43801 aattacattg agaggattat caatatttga acgcataaaa agggacgttt tgattgtaaa
    43861 aaattttttg ctgaaatcca tatacatact tctatctttc gtaatgaata atccactatg
    43921 tatatctgct cttttttcct taatcatgtt aatactttct ttccacggag catgaattat
    43981 ttttacagga attccggttt ttgccgacca tagattccaa aaatcaataa tcattccacg
    44041 caaaattcca cttttatcaa tataagaaaa tggcggaaat ccttcggaag ttactataat
    44101 aagaggcttc tcagcactat atatctgtgt aacaaaaaaa atattaatta gaaccactaa
    44161 taatattaac aatctaatat cctttatttt tgatgttttt tctgataaat tttttatcat
    44221 ttcacgaatg cctctattct cattacaaca taggatagta attatttata gataaaatac
    44281 ttttattaca aagtttattc ttttgcaaat gattaacaaa acaaactaaa ggagaaaacc
    44341 ttcaacaata atcataagaa ttagtaaaaa tgtccactca taaacaactt ttaataataa
    44401 ttaatacaca atgaaatctt ctctaccaaa aaataaacaa ttttaaattg agtaaaaaat
    44461 attattgaaa taacaatctt ttgttcataa atttttattg aaacatagta tacactataa
    44521 cactttgaaa gaaaattata cagagcatcc tgaacaataa gagtgttact aacttaaaaa
    44581 atatatccac ataggaggct acctatgaca caattacata ctatcaacaa ctatggtaca
    44641 gcaactatca cccgtcatga tgctttgctg gttatagata tgcagtatga ctttatccct
    44701 ggaggctcat taccagttgc cgaaggcgac actatagtaa agcccatagc acacttaatg
    44761 gaacaattct ttataaaagg tgcaaccgtt gtatgcacac aggattggca ccccagcggt
    44821 caccgttcat ttgcatcaac tcatggaaaa catccttatg atcccattaa tgaacctggt
    44881 attggaccag tgctgtggcc agatcactgt gttataggca caaaaggggc aagccttcat
    44941 gacaagttgc ctgcacactt ttgtcatgca atagtgcgca aaggctacaa cataaacatc
    45001 gatagctatt cggcatttct tgaaaatgat aaaaaaacag caacggggct atcgggatac
    45061 ttttcaaacc gaggcgtaaa acgaatattt gtatgcggac ttgcgtttga ctactgtgtt
    45121 cattttagtg cagttgatgg cgccagtgct ggatttgaag ctgtagttat caccgattgt
    45181 tccaaggcag tcaattcacc agctacaagc gtggatgatg ccattgctaa tatgaaagcc
    45241 cacaatgtct cattttgctc atcagctgac ctttcattct aatggcaact caacggtaaa
    45301 ctcagcatat tgtcccaaca ctgagtttgc ataaattctg ccactgtgca ggtacacaat
    45361 attccatgac acaaaaagcc caatgcctga ccctttgcgg gcaagcagtt catcagtttc
    45421 aatgcgcgaa aaccgcttga acagtttatg tttctctgat tcaggaaacc ccgggccagt
    45481 attccacacg ctgattttgc atatagtgcc ttcttttttt acggttatac gcacaatccc
    45541 gttatcacta ttatacttga ttgcattgct taagaggtta tgcatcacaa tcttcatggc
    45601 gtcgggatcg cactttagct gtacatcctc ataatctttt tcaacggtta tatgttttgc
    45661 atcagcctgg ggctgtacaa tggcaagtgc aggttcaata atatcatgtg cgatagttac
    45721 tgttgatttg cgtactggca ttgaaccagt ctcaaggcgg gctaagttca aatactcatt
    45781 tactatcgtt aacagatatt ctgcctgcgc aagaattcga tcaatggtgt ttgtgcaatg
    45841 ctcagggatt tctccaaagt atccctgctt taatgtggta cctaacgtta ctatagatga
    45901 taaagggctt tttagttcat gagttacaaa tgatagcatc tccatgtaag cgttgattgc
    45961 ctgggttaac tgctcaatgc ggtatgcttt ttcaacagcc tggctcagtc gctcagcaat
    46021 agcctgatgc agtgctatct cacgtaaacc atacatattc tttttacggg aactcctgaa
    46081 caacacacct acaatgcgcc cttccacata tagtgggcag gtcatgcttg acataacacc
    46141 ttctttcaaa agcaggcgcg ttgattcact tgatggatta tttttgtaat actcctctaa
    46201 atcattaata atacgcggtg tttcattgct gaatattgtt tcaagagaac tccccttaag
    46261 gtcagcagta tatcctttat ccaaataaag agggctgtat gaggctacaa cgtggtacag
    46321 cgttaagcgc ttgccatcat cagaaaatgc aataccaatt ctatcacagg gcatgatatg
    46381 ttgtgtttct ttaaataaat attctatgat gtcaatgagt gattgccctg aagctatctt
    46441 ctgattaatg gtatctaatg ttttaatttc ttctggtgtt aaaacaactt ctccaccttt
    46501 accctgtaca taggaaattg tatcacgttt gtccatgcat cattcctcat tacatttacc
    46561 atgaacaagt ttacagtatc catacaatta tgcaaggttt ttatgtagta tcattttcat
    46621 tgtatttgta atttcatttt tcctatgttt ttaatttttc actaatcatt tatatttaat
    46681 ttcaaacatt atctaccata attatcaaaa aaagcatcac atcgttttta atgtttttta
    46741 cacaaaatct tttttatttt cgtaaggggg tatttatcac taaatacccc cttactaacc
    46801 cccaaagtgc gcctcaaacg gcggcgtgcc ttacttgcta tgtagtaatt attccacggc
    46861 gggctgcgga atagcatccg ttcaggtcct cgcccgcatc ccaatttgcc gtacttcctg
    46921 tacggcaaaa ccttgcaccg ttcttattat aatattcaaa tcattacttc atattctcta
    46981 aaatattgac aataaaagaa agccttgctt cagaatgtgc tgtatgtgag gattaggaaa
    47041 aatttttcat tagtaactat cgcacaaaaa aactactcaa ttatttatac gtttgaaggt
    47101 actggtgcgg tatccatata gtgctttgct atgatgaggc gttgtatctc acttgtccct
    47161 tcgtagatgg tggtgacgcg cacatcacgg tatagacgct caaccgggta ctctctggta
    47221 tagccgtaac ctcccagtat ctgcatggca tcgtaacagg ctttgtttgc aacctctgtt
    47281 gcaaatagtt ttgccattga ggcttctttc atgaacggca aacctttttc ttttttatag
    47341 gcagcctgaa gtaacagcag gcgtgcagct tcaagccgtg tatagttatc ggcaatcatc
    47401 cactgtattg cctgattgtg tataataggt ttgtcaaatt gtatgcgctc ttttgcatag
    47461 ctggttgcat aatccatagc agtaagtcca atccccaatg ccattgaacc aatgccaatt
    47521 cgcccgccac ctaactcacg cactgctatc ctgaatccat cattgagtgt tcctagtaga
    47581 ttttcctttg gaactttgca gttatcaagg attatctcgt tggtagagga gcccagttgc
    47641 cccattttat gttcatccct gccaacaata aatccttcaa aatgcggttc caccaaaaat
    47701 gcactgatac ccctgccttt ggctgcgtgt ttattggtaa cagcccacac cacaaaaact
    47761 cctgcatact ccgcactggt tatgaatagc tttttcccat tgagtatcca ccagtccccg
    47821 ccatctgttg ccgttgtctt cattgccgcg gggtcagagc ctgcctctgc ctcagtcagg
    47881 ccaaagcttc ctgcataaaa ggtaccattg caaagtggtg gaatgtaccg ctgtttctgg
    47941 tcttccgtcc ccaccgattg aataacctcg gccaccatat tggttactga tgttgttaca
    48001 gcagtggctg cacaggcttt gcccagttct gtcatcacca gtgaaaacgc aacgctgcca
    48061 gcacccgtgc ccccataccg cgggtcaaca gcaatgccgt taaaacctaa cgctgccagt
    48121 tttttcaggt tggattttaa tatgtttctg tcttttgttg tatccagtgt ggaagcaacc
    48181 ggttcaagtt cctgttttgc aaatttagcg gccgtttcct gaattatttg ctgttcctgt
    48241 gttaaactga aattcattgg caccacctct tttatgtgta atcgtaaaac ccttttccgg
    48301 ttttgcgccc caaccagcct gattccacat agcgcctgag gagcggacat ggcctgtatt
    48361 ttgaatcctt aaaaccatca tacagggttt ccataatagc taaacatgta tcaagcccaa
    48421 taaggtccgc cagtgccagt ggccccatag ggtggttcat ccctaatttc ataactgtat
    48481 ctatggactc acgagttcct acgccttcgt acaggcagta tatggcctca ttaatcattg
    48541 gcatgagtat acggttggaa ataaatcccg gataatcatg agcctcagcc ggtgtcttcc
    48601 ctaatttgag cgatagttcc caggttacat caaatgtttc ctgcgatgtg ggcagtgccc
    48661 gtataatctc aacaagcttc atgacaggca ctggattcat aaaatgcatc ccaataacct
    48721 tatcaggacg ctttgttgct gcggcaattc gacctatggg aattgaagaa gtattggtgg
    48781 cacatattac ctgtggcctg cagatggtgt ctaaatcctt aaatattttt aactttatat
    48841 cctgattttc ggtggcagct tccacaacaa aatcagcatc tgcaagtgca tggaactcac
    48901 ccgttgctct gaccctctcc attatgcgtt gcttttcatc ctgcgttatt ttcccttttg
    48961 caacatcttt ggaaagcaac ccgtttatat acgttacacc ttttaaagcc aattcttctg
    49021 ttaaatcata gagaataaca gtgaatccgc ttactgctgc aacacaggca atccctgagc
    49081 ccatctgtcc tgcacctaca acacctattg tattaatggt atctggtttc atacacattt
    49141 atctcctaaa atacactatc gtttcacaat taatgcaact gcttcgccgc cacccaaaca
    49201 cagcgatgcc aaacctatac gtttattctg gcgtatcatt tcatgaagca atgttaccag
    49261 aacacgcgcc ccacttgcac caatgggatg ccccagtgca acgcttcctc cattgacatt
    49321 gacctttaaa ctatcaagac ccagttcttt caacacacca actgttgatc cactgaatgc
    49381 ttcgttgatc tcgtaaaggt ctacatcctg caatgatatc ccttcttttt taaggcattt
    49441 agggatggca agtattggcg ccatcaggac gtatttcata tcaatgccag ctgaagcctg
    49501 tgcaccaatc gttgccataa tgcggcatcc caactctttt gcctttttct gtgacatcac
    49561 caccagcgca gcagcaccgt cactgataat ggaagcattg cccgcagtgg taacaccatc
    49621 ttttttaaaa gcaggcttca ttgttgccag tatttcatac ggtgttggct ttgggcattc
    49681 atcggtatca aaaataattt ccttttgttt ttgggcgata gttaccggaa ctatctcctc
    49741 cttaaatttc cctgtctgga tagcatgtag agctctttca tacgaaagcg cagcatatct
    49801 gtcctgctct tccctgctca cattatactt ttcacagcac aattcgtttg agatacccat
    49861 atgaaaatcg ttgaccacat cccataatcc atcatgaacc atatggtctt tgagttgacc
    49921 atcacccatg cggtacccaa accgtgcttt ttccagatag tagggggcca tactcatatt
    49981 ttccatgcca ccagctatca ctacatcagc atcgccacac tgtattgcct gtgctgcaag
    50041 cataactgcc tttaaaccag agccacatac tttatttatt gttaaacatt ccacttccca
    50101 gggaagatgc gccttgattg cagcctgccg tgccggattt tgcccataac cacagggaag
    50161 cacctgcccc ataataactt cattgacatc ttcttttgaa attcctgcgc gcttaatggc
    50221 ttcttcaata acaatggctc ccagttgcgt tgcgggtatg gaagacaagg aaccgttaaa
    50281 attacccaga gcagtccgta cagtactgac aataacagct ttcattggca acctccattt
    50341 ttgtatatca ataatacata aacaaaacta ttcattatat agtcaaaatg tcaaaaatat
    50401 ttaatggtaa atgttaaaat tttcaaatgt accacaaaat ctatatatac atactgtatt
    50461 ttcattatat tcagctatat ttattcaatt tctgtgttgc ttaaaacaat tttgcttgca
    50521 aaatacttta tattaacgta atcataatac tataaaatat aaactttgga aggatagtta
    50581 tgaacattga aacaaccaca tgtatttccc atgaacatct agaactactt acattacatg
    50641 ccaacaatca ttcaatgtca ctgagaacgt ttatctcttg cctcattagc tttgctgcac
    50701 attgtgatac tgttcaggta caatatttca aacaggtaaa atataggaat agaaatagtg
    50761 gcaattggaa gaggtttcac ttagtactct ataacgatga gtatgaattc tttatggatg
    50821 tcaaaaagtt atggaagatg tcacttgctc ggattattga gtattgttta gacaatgtac
    50881 tgtatgattt tttaaaattt ttaacaaaag ctgaagaaga tgaagattac tataccgata
    50941 actatcggta tggaggttac gcttttgaga caggcactaa ggaaaatata ttttatataa
    51001 cattgtattg gggaccacac cccgaaatac tccaaaaagc tatgacctga taacgtaaaa
    51061 acactattca cgtaatacat cattaggttt ctatagataa atagtttttc tcatggataa
    51121 aattatatat atgtagggca tactatttct ttttttaatt tttaaattga ctaatttaaa
    51181 aatagcaatt catatttaaa tagtagtaat ataaatattt cattttgcat ggtacattgt
    51241 tgtactggat tactgttagt tgtatcgata gtataatgat attagtaaaa ttaacatttt
    51301 taaggggtaa taatgatggc aaaaaagtat aaagcagtta cttgtattgt tttggtacat
    51361 atagtgttta tattaatatc atgtacatct gttgagtttg ttaaaactgg tgaagaatat
    51421 cctgcactca atgaaactga tgaagttcag gttttgctta ccatagataa aaaccaatat
    51481 gatgagatag gcatcgctga tattggcgga gtatcattgc ccgtacgtat tgaaaaagcc
    51541 aaacagattg cccgcagcaa aggtggtaat gtgattgtcc cagtgacaat gtgtactgaa
    51601 gaagcccaga agagaatgcg cagcggttat attattcaga gcttttatat actcaaaaca
    51661 aaaacggaag ctatcgcccc caaagaggca attgaatcaa aacagataac tccaccagtt
    51721 gaagaagaat ttatacccga aaagcaagct gcaacatttc aacaactgtt acaacactat
    51781 ccgtcattgc agggacagcc atttacaaat actatggtgc cactgagatt ttacaaaatt
    51841 cctccatcac ttcttgtatt tggactgtca gactataaac tttgtttaat gcagtttgac
    51901 actgataata ctgtactggt atttattccc aaagaaaaaa tcactgttat taaaaactct
    51961 attgatacga ggagtactat agctattgat tttacacctt taggtgttta caaaggaaaa
    52021 tatcccgttt taaaaatgct gtcagaataa tataaaaagg cactgcatca tggtacagaa
    52081 agtttctccc cagagtagcc tttgcagatt tactctcaat gatgaggaaa aattatccga
    52141 tgaaagggta aaagaagaaa tacgcaaagc ttttcaagag ctcagagcat tcccctttgt
    52201 tgtaaattca ggtattatcg attcattaaa aaaaggtgcc gacccctcat cgttaaaaga
    52261 tttatttgta gctatccgaa atgatatatt tgctgcaatt accgatagtc cccaggcaaa
    52321 agaaaaaata gtatccgaca ccagattgca ggagagaatt ttaaatgcaa ttatcacaat
    52381 ccacagtgct atagcaaagg gcgattttac ttcagtaata aaccgccgct ttgtgcttat
    52441 caaggaacga cctgcctggc caacactata ttacatttca cctcaaagtg ccgtactatc
    52501 ccacgttggg cagggtccac agtggaccga aattcctaca atttatctgg gtcttaatat
    52561 attcaatgtg cttgcccttg ataaagatca gaaatttgac aaagcatttt actggttatt
    52621 acgggttgaa gagcgtgcga tagaaactgg atattcgcat attgaagtgt acccgcctca
    52681 tgtatcccgt gctctcaatg aattgcttga tgaagttata cgcattgcaa ctatctcaga
    52741 agttgaattc aaagaaatcc ctgcccgtaa aaaaatacgt gcttttacaa tccgtaaccg
    52801 tgaaagcttt ttaaagatgc tcaatgcccg tcgtgatgat ggcaactttg attttgaaaa
    52861 aaacttaaaa gcaatacgat cactggaaag gctggcacgc cgttacaaaa aaggctacga
    52921 cttcaattcg ttgcgtgaaa ttgtacgtct tctggtggcc gcatcaggcc atgatatcca
    52981 tgaaatacga aataacgcca acattttact ggaacgcata tttgcaccaa aggagtttga
    53041 tgcaccgctg gctaatactt ttgtcaactg tgccgcaggt agtattcata catttgaatt
    53101 ttccatcccg gaagaaggaa actactttgt gcgtatattt tacaatgcag gcacaaaccc
    53161 ttaccctctg gagcaggaca ttgcatatag cgacattgat ctggcctttg atgaaacaaa
    53221 aagagtctat agtgcacact atacattcaa cgaattaggc cactatgatt tccttgtcta
    53281 cagaaaacga aaaaaacgtt ctcagtgggt aacatatgaa ggttgcagtg gtaggattaa
    53341 cgtcatccct gacgtgcgcg gtgaaattgt tcttgaaata tttcccgata ttcacgggca
    53401 tactcgtatc tactggatgg atgaagaaca tccgggattg gtgtacaatg agcatggaga
    53461 agtaattcgt ttaggccgtt ttagcgacat cactgctcat ctagaggatt taaaagagcg
    53521 ctatcacatc actgctctgt accttttggg tgtccagaag cgtggtacca accgtgaaga
    53581 ctgggcacct gaagctacat caccttcacc attttcacca gtcagtttag ttgaaattga
    53641 agacttttta ggcggtgaaa acgaatttcg tgagcttgta tccaaagctc atgcaatgga
    53701 tataaaaatt attattgatg tggtacccca ccttaatcgc cgtagcactg agcttccgga
    53761 tgagtacgct gtcaaatgtt acgacgacag tggcaatctg gttattcgtg cttcaactga
    53821 tggtcgttac ggaagctgga atgacggcaa actgttaaac tatcgcaaac tggaagtatg
    53881 ggaatggctg atcaacagtg ttgttacatt aattgataaa tatgatattg acggcatccg
    53941 ctttgattca gctcatgcgg tccccatcat gatgaaaaag aataactatc cgtttatatg
    54001 gggacagtat cgttcactgg aatcgcttgt tgaaggtgaa attattgtca atgatcgcga
    54061 agatgggcat tttattacaa caggttattt tgacagtgcc tgcagggatc agatagctat
    54121 ccctttccat catcttctga tgtgtcgcat tgcacaaaag ctgaaggaaa agaataaaac
    54181 attttttgtg catttagccg aatgttactg gggtcatgag cgctatctta cccgcagcgg
    54241 aattattccc tataattcgg ccctttttaa gatatgtgaa gggatcatcc atggcaccac
    54301 tgatgtccgt gaagtatacc atttttacga caactatctc ccatacgctt taccgccggg
    54361 tactgagctt ttaggcatac ttgggaacca tgatgaacga cgcgcactca atacgtttgg
    54421 gcaccgggga ctcagggctg ctattggact tactatattt atgaataaca tcattatgga
    54481 ttatgaggga agtgccgaag gcgaaagctg gaaagtattt ctggataaca tctatgtaaa
    54541 ctggaatcaa tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    54601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngaaggcgaa
    54661 agctggaaag tatttctgga taacatctat gtaaactgga atcaatttga gtatgctgct
    54721 caccgcagcc tggagtcatt ttaccgacaa tggtaccgtt tccaccgcat caataaaggc
    54781 aagggatatt tgatatgggc taataacact caggttgcag catccattaa atttaccgaa
    54841 catactatat ggattggcat ttttaatttt gccgatagct ctcagaatgt ggcattgcag
    54901 tttgacaacc cgcgattgcc cattgctgat gatacatact ttaaagtagt tgatcctgta
    54961 tattcaccta tcacaaagca ttacagttac tttacaggga aagagcttaa ggcttcaaaa
    55021 atttattcgg ttgtttcata cactgacagg atcaagcttt taaaattaga accggttagc
    55081 gatgttgcac cactgtacag tgaattcctg cgcgattcac ttttcagatt atattcaatc
    55141 agcaatcccg aaaactttaa atcaaatttc atgttccttg aaaccattgc acattcttcc
    55201 acttttgaag catttcttac atttttaaaa aatcacatca ttgcacaatt ttatccgcag
    55261 tataaaaatt tcattgagat aggttttaaa cgaatcctct tctacatgtt taagtttgga
    55321 ttcaaatctg gcaatgacat tgtgcaatta attgatgacc ttgcagaaca tgatgatacc
    55381 aatatatccg accttggtaa aagcattaag tttcataacc gtccaggccc cattatattt
    55441 gtatcagccg aagcggagcc attttccaaa agtggaggtc ttgctaacgt tgtgtacgag
    55501 ttgccacgtg aacttgtaag tctgggtgaa gaagtaattg taataatccc taaatatcgc
    55561 catggcgatg aaaaggccat ggaaaagatg aacaatgcat taaaaaaata caatgtacag
    55621 tacactggta aaaatgtgcg cttcatgata gaacatgcta cctatgaagc tggtgtacat
    55681 tatgcacagg ttgatggcat tcattattat ctactggatc atcacgagtt ctttgatggt
    55741 ttgtactggg gatataccgg tcaggaaaag ttacggcgcc gcattgcatt tgcccgtgcc
    55801 acagcggagc ttatcaccac ttttggtctg tacccacttt ttgtcatcac caatgatgca
    55861 tataccggta ttttcaatgg cattgtccgc agtgaccatg tgtactacga taacccaaac
    55921 ttcaaaagaa catcgttctt tcatataata cacaatggtg gctggcaata ttttgattca
    55981 tatcaccgat atgaagatgg caaagacctg ttctctttat ttaatcttcc gcactggcgc
    56041 tataccgatt tttctgaccc caatgattac aacaagataa attgtatggc taccggcata
    56101 cgatttgccg atagagtcat tacggtaagc ccttcatatg caaagcagat tgaaaaagcc
    56161 tgtgatggtc ttgaaaaaat tctgcacaat gttataggta taagcaatgc gctgggagtt
    56221 gattttaaaa accgaattct catgcgtttt cacaattcag gttttattga tgaatattat
    56281 cccagaatgg ttgacgcatt aacatccagc acagcaacgt ataccaaaat aaaacagcgc
    56341 tatcctgaaa tacttgaagg catacgcaat tgtgaaaaga ttaccgatcc tctgcgcagg
    56401 gagtatgtaa cccacatccg gaacaaactc tgtttgcaat gtaaattagg ttttgaaatt
    56461 aatccagata taatgttgtt ttccatgatt caccgtatag ccgagcagaa aggttttcag
    56521 cttcttctgg atgcttcata tgggatattt actacactgg gatttcaggg ggttattggc
    56581 ggtgcagtag catgggggga ccagcgtggt agtgaattag ctacaggctt acttcatttg
    56641 caaaactact atccaaaaaa tgtgaacgta aacttaggct ttcaggatat cagcatcccg
    56701 cttctgtgca gtgatgtgtt tttaatgcca tcattacatg aacctggcgg catttcacag
    56761 cttgaagcac ttgcatgtgg atgtttggtt gttgcacgcg caacaggagg tctgcgtgac
    56821 accgtaattc ccatacgcat gaaagattca tctgttgaag gtaatggatt tttattttct
    56881 gattttactc cttcatcgtt ctatgatgct atgcaacgct gtgcagaatt cttacgtacc
    56941 agcgatgata caacaaaagc actggcacga aaaaatgcca tgcagtcagt ctattactgg
    57001 gataaaccgg caaaagaata tattgatgtc atgtattcac ataaggaaat tgtaagggta
    57061 gtataaaata aaagagcctt ccacaaacac catcagtaca agtgggaggc cctggagtgt
    57121 aaaatgatct ggtaatttaa tcggaagggt aatggtaaaa cttgaatgtt ttattgtgtt
    57181 atgaaaaaaa aatatattat tgcagtaatt tcattatgga tactatcctg ctcctcaaat
    57241 gataaagcag agttggggaa tattgttggc tctatcaatg gacagccggt tataccctat
    57301 gctgctaaca aactctacat atcaccattt acgaatcaga ccggtatcct gggctatgaa
    57361 gaaaagataa aacagcgtat aatggaaaaa ataataacag gtggcaggtt agcgatagct
    57421 ccagtacagg ctgatagcga tgtaatattg acaggcacca ttcagtcatt tcaggtgcag
    57481 ccaattgtga ttgacagtgc aggaataatt aaaaaaaagc gattattact aattactgtg
    57541 ataagtttaa tcaatgcaca gacagggaat tacatttttc gcagtatcac cgtacaggca
    57601 tttaaagagt tttcggacat tgaagcacct gttacaccaa ttaccattgc tcaggatgag
    57661 gtggaacagg ttttagcttc acggattgct ttacaggttg aaacaggctg gtacacttcg
    57721 cttaaaacag gagtggaaaa aggaaggaaa tgatatggca tcaaaggcgc tcatggctaa
    57781 cgacctgaaa tctgtcattc aaaaagaact atcacacata tactgttttt gtggaaacga
    57841 tgaaaaccag aaagaagaag cggttacact cattgcatca cagctatgga aaaatagtaa
    57901 acctcaagat tataaaatta ccacatactt tgctgaagat tcgcttgaac aggctcttgc
    57961 tgccatagca tccgtttccc tttttgacga tactgtttta tatataatca aacattgtga
    58021 gcaggtaagt ggttcaaaaa aaattcatac tatggtactt gactgcatta ataatctgcc
    58081 caactcagtc ttcgttattt ttgatactca ggagaacaag ctgccctcat tgcttccttc
    58141 acaaattcat ccacacattc cccttattca gttttacaga ccatttgaag aaaactgcaa
    58201 atcttatata ctgaaaaagt gtaaggattt atcattaact atctcacatg aagcaacaga
    58261 acgtcttgtt gaattaactg caagtaacat taaagagatt gatgatattc ttaccaccat
    58321 atcattttct ggccatactg atatctcaaa agaattcctt gagggttttg tcattaatga
    58381 aagagaagca atagagtttg aattcgttga ttgcttcttt ttaaaagata aacgtgcgtg
    58441 ggaccttctt tcagaagcca ttgaacgtaa tataagtcaa attttgttag tatccctttt
    58501 atttaatcat gcacaaaaat tagaacaatt tcatatacaa acagcaaaag gcgttcagtt
    58561 tgagtcactt atgaaagaat tgaagatata tggaaaaagg caaaaagtgt ttgccaaaca
    58621 ggcatctagc tatacccttg ataatgttag aaaattaatc gcaatgttat atacttatga
    58681 tatacagctt aaaagctatc actcaggagg ttttcctcag gaaaatccac tattagacct
    58741 tttattgcaa atttctatgt gatggcactg tatctatgga tctgctccaa acaataattg
    58801 aaaataagga aaaattcata gcgtttaaca acgaaaagtt taagcgcttc caacagctta
    58861 ttcccaatcc cgcaatgcgt cgaattgtaa acaccatccc ttttttactc tgcatcaata
    58921 ataaaaagat gcccggttac gttgaaggtg atgtgccttt aggaataaaa aatttcaaac
    58981 ttgatgaaga caccaaacgc tatttgcatg gacgttatcc tggtattacc ttccacgatt
    59041 ttgaacgtgg cgattttatc aagatgtttg ctgttatggg cagtgttggc acagtagcat
    59101 ataataaaaa atcagacctt gactactggg catgtattca taaaaacact gtatcaaaag
    59161 aagcattcga taattttaga aagaaagtaa cccttgtaca ggaatgggct tcaaaagaat
    59221 tagaagtgcc cgtacatata tttattaacg atattgaaag cgttaaaaat aatatctttg
    59281 ctgaagacga agaagaagca tttggcacta ctgttggcgc agttctgaaa gatgaatttt
    59341 tccgaagttc aattatcatt gcaggcaaaa ttcctttctg gtgggttgtt ccaagtttca
    59401 tcaaggatga tgaatatgaa gcattgtaca atcaactccc tgatgaaatg aaaagggatg
    59461 attttattga tctgggaaat ctttatgaaa tatccaagga agattttctg ggcgctgctc
    59521 tttttcagat aataaaatcg ctgggtaacc cttttaaatc cattttaaaa attggagttc
    59581 tggaaaaata tctgtttgga aaaagtgatt caccactgtt aagccagaag gttaaaatgc
    59641 atgtactgct cagccgtctt accaattcaa tactggatgc ctatcttttg atgtttgatg
    59701 aagtttatga ttactatagc cagacaatga atgaccccgc catacttcaa atattaaagc
    59761 aaaaccttta tttaaaaatt gacccgcagc tatccaaata tgtggggata aaagataaaa
    59821 agaatattcc ctataaagta gctgtaatgt tcaagtatgt ccatgactgg aactggaata
    59881 ttaaggttat tcaggatatg gataactttg acaactggga cttccagcga gtaatgcatt
    59941 tctgggatgc agtaaaaaaa ttcatgctga tcagttatca aaaaatatca tcacagttta
    60001 gtacccttaa tctaaaacaa caaatatctg aaactgattt tacattatta tcacgaaaaa
    60061 taaaatcaca tttttcggta gaagaaaata agattgataa gcttataaca tttaaagaca
    60121 ctcctgccga accaatttta tatattgaac ctgttaacga aggtatacgc gaagttaagt
    60181 ggcggctgtt taaaagaaac cgcagcgaaa aaaatgtgtt cactaccacc acattgcgta
    60241 ctgaaagcga tctggttaag ctattagtat ggttaacggt aaataatgtt tatgaccatt
    60301 cagtgacccg tgttaatatt cagtccggat atgtacgcat taaccagaat ctcgttgttg
    60361 aactgctaaa ccagctatca caattttttt ccgaaaaagg gaaaattaaa acaaaatact
    60421 atctcaaacc agcttttaac atgcttcaat gtattattct caactttaat aatgatgctg
    60481 cggaacattt agattcatta taccatgtct attcaacaag ctggggcgaa caatacatca
    60541 aacaatactt caatatcatt gatattgcac acatacttca aaatatcctt aaagacggtc
    60601 ttgaattaaa actccccttt gacgattatt gccttataaa caccccgggt acccacaaac
    60661 gcttttgcaa aaatttcata cagcttttta gagaatcata taacgccata gttcacagcc
    60721 ctaccccgca cagccgtttt attggcgagc ttgaaggcct gtattttact gcattacgta
    60781 acaatactat tgaagttcag gcatttgatc atattgtaaa ggtattaaca acactgacat
    60841 taaaaccagg tatagaagct cattatacat tctatggtga ttcataccat ctgcaggtgc
    60901 tgcaggcatt gtataacaaa cgacttaaaa aagggattac aattggttat cttgaagaca
    60961 gtgataaatt gcatgtttat gtaattaatg aaaaaggtaa tatttttact ttctttgcac
    61021 caaaaaatat aaaaagcgtc tttctatatc atatctatgg attttgcaca aacgtcctca
    61081 aacgattgcc aagcagtgta agggaaagtg agtccagcat catctgctat ggtgctcagg
    61141 ccgaccgtag aggtggtatc agccttattg atgaaaccgg taaaatacat ggtgattatc
    61201 tactaaattt tacaaaatcc aaagctcttc gtgttcagat agctcgccat atggggaatg
    61261 aaatttttta caactttata tttcccgaca atgtaacttc cggatttatg acaattaaag
    61321 aactttatag cgtaaaacgt aaaattattg agttaaaaaa tatgggaatg ccagtatatc
    61381 cttatattgg tgaacttgta tttaatgatc ttaatcaaaa agaaatatta ttaggatcaa
    61441 gcatatattt tgtggaaaaa tataaaatag aactcatgct gcagcgaaca caagcataga
    61501 aaaactcaat attctccgcc agggaaatta tccttaaaaa agcgtatttt ctttttctgg
    61561 gtttcaactt cctgcttatt gattgtgcct ttttccaaag ctatatccag taattttact
    61621 gctgtatcgt cctcatcttt ttttgcttta tcttcatcat cctgctgtaa ctttgcaatc
    61681 tttctatcaa tatcatcctg cgttaacatc gaaaattcct gttcttccag ttctactact
    61741 gtgtctttaa ttttattttc ctttggctct ttctttttgg aggttttctt ttttggttca
    61801 gcaattaaag cttcactaac ttcttcctct tctggttgca aattggctgg tggatttgct
    61861 atttttaact ctaatagttt tatgcgttca cccaataaaa tgtttatctg cttttcaatg
    61921 gttataaata taggctgagt aaatgattta ttcctttgca aagacttttc caattttctg
    61981 agttcacgaa tatcctggtc tatcatttca attcgtttaa gcacatggat aactttcata
    62041 atcaaacctc aaaagtaatg ttaagttatt tagcctgtaa cacaatcacc ggcaacacca
    62101 ttgtggttgt tatacgctgt gcattaaaaa gtttataata caccgctaaa gtaatgtaca
    62161 ttaattataa cattaatgtc aataaacttt taatactgga aaagatttta ctaattaaaa
    62221 taaacagtct ggtcatcatc cactgtaaac tgcaacccgc aatcggggca tttaaattta
    62281 cctggtgaac ttatccgtac cattgttcta caatcaggac attcaatcac caatggatgt
    62341 tcaaacacca tggaggttct tgcaactggt tttgtttccg tatatgtttc tttttttggg
    62401 ctttcttttg gtggcaccgg tttttcaata atttttacct ctgtatcttg tactgcttcc
    62461 attgcttcat ccaatgaatt ataccgtttt accggaagtg caatgatatc aatcaatgtc
    62521 actatctcct tattacaatg gcagagcacc accacaccgc caccagattc aaccaccctt
    62581 tgtgcataca ttaatgcgcc aaaccctgtt gagctgatat agttgagttt tgaacaatca
    62641 atgattatag cattgaattc cttttgaagt gttgcaatat actcttcaaa ctgcactgca
    62701 ttggtatgat caatgtaccc ctcaacagta actatcgcta ctgaatcctt tactgtatgc
    62761 tgaataaaca tttgagtcta ctccttcacc ggtatggctt cttttattat ggagcgtatc
    62821 tgaccaggct tgtcatgtat gtgagcactg aattctttaa cttcttgcaa tacccttttt
    62881 tgtttaataa ataaaacaat accagtacaa agtgaaaaca ccagtgccgc cccacaaatt
    62941 attatgagca tatacaacag ttccttataa taactgttca tcatatcata gataggggtt
    63001 ttgacaataa tgcaattaat ggtgccaatg gatttgccct tagcatatac agcctttgtt
    63061 accagccagc tgttagtatt gatatcttta tataaaaact gctgtatata ctttctgata
    63121 attgttgtaa atggcaaagg caggtcagga acaaaataat taaagataaa aagttcccgg
    63181 gattgttttg tatgaggata cagaatcata tcgatattgt aataaaactc atcattggca
    63241 atgttccctt taagctcttt tgctttttct gtgtcagaat gggcaattat tgtgccatta
    63301 tctaaaacaa aaaagacttc ttttattcct ttaatgtttt ttgattcctg tatgagcgat
    63361 agtaactggt cagtagacct ggttgcatat gccagctctg cagcatcagc atatgttgta
    63421 atcaacgttt tagaaacatt ttttgcctgg attgtaaact catctatttg cgataccagt
    63481 tgcctgtatt tataacaaag ggcaccagat gaagctagta ttgctataat aagaaataga
    63541 actataacat atgcataggg gaactgtact tgtttatcgt tttgcataat tattcactgt
    63601 gcttttaaaa taccttgcaa tattttacac catagatgta ctgttaatct gcttatgata
    63661 gcaatcaaac tatagaaaca tgatactacg catttttata tttatcggat aataatgcgt
    63721 tgatatgtat actttttcac tggagaaaag gacgatggca attaaggaag aaatcctgga
    63781 agaatggaat tgggaacagg gagtcccaac aggtaaacct gtagcaagga gcattgccca
    63841 caaacagggt attgcacatg aaggagtaca tttatgggtg gtgcgtacac aaccccagaa
    63901 agagatactc tttcagatgc gtgcatcgca caaggatatg tacccggatt atttagacat
    63961 taccgtagga ggtcacgtac cttttggact taccagtaat aaaattcaga aagaagcata
    64021 tgaggaaata ggaattacgt ttgatgaaaa acagcttatt gatttaggct attaccggta
    64081 tgaagaaatt aatgatcttc ttttccaccg cgaattccag cgtgtttacc tgcttctatc
    64141 aaataaccct ctttcttcat attcgttcaa cgataatgaa gtgagcagta tttacgccgt
    64201 gccgctggat tactgcaaaa atctttttat caaggattac tctgtaacag ttgaatcatt
    64261 taacggaacg gatactattg ttacaacaat atcaaaaaaa gattttcacc ctctactttt
    64321 tgcaccatcg atggaacgtt acatggaaat actatttcaa gcaattgatg agctttttac
    64381 aaaaggcaca gtgagtgttt caatgtctta ggttaattgt acagcaatga gtgtacctac
    64441 tatttcatat aaggaaaccc ttgtatggga aaatcccgtt tatgttgatg tccgtgctcc
    64501 agttgaattt gtaaaggatc atatccccca tgctatcaat tatccgctct ttaatgatgt
    64561 tgaacgagca tacattggga cgttatacca tactcagggc aaggaaaaag cacttctgga
    64621 aggcgtgagc attgcctcat taaagattag agatatgtta tcgttttttt ccactttaaa
    64681 agaacaccag gttatcattt actgttacag gggaggaatg cgttcggaat cagttgtatc
    64741 attatgtaat tccataggat tttcttttta taagctttat ggcggttata aagagtaccg
    64801 ccactatgtc aggaattatc ttacagaata taccttcact ataccagttt ttacactgta
    64861 tggctttgca ggttcaggca aaactaaggt cctcaaaaat attccatatt caatagacct
    64921 tgaacattgc gcaggcaaca ggagctctct ttttggtagt ttaggtatgc ccaatcacac
    64981 gcaaaaatat tttgagagcc agttagttca agaactacga acgattgaat catggcgata
    65041 ttgtattttt gaaggagaat cacgaaagat tggcaactgc catatcccac attgcgttct
    65101 caattctttg caaaatggga aagctatatg gcttgaaaca ccactggaaa aaagaatcca
    65161 gcatatttat gatgaatata tgccatttat acgtgaagaa acaatattgc caatattaga
    65221 taaattacag ccaacaatgg gaaaagcaaa aacagcctat tgcaaacaac tgttcaacca
    65281 gggcaatata catgaactaa tagaatttct gctactgcat cattatgacc tagaatatcg
    65341 acatagtaca aagaatattt cttttatggc ccatattctt ttcaaggatc cagtgactac
    65401 tgcttctgaa attgatagtc ttattaaaga catgcttgca gtataatatc ttttttttgt
    65461 tgactacaat aataaaattt tgtattgcat atagtaacat cgtaattact gtattgtaaa
    65521 catagaacac aatacaaata tcatattgca ggaggtcatt agtcatgaaa aaattatctg
    65581 tattgatact agcaatgctt ttgggttttg ttgcctgtgg aggcggtgtc cagtatcagg
    65641 atgcaagtaa agctgaaggc tcaagagaat gggggccaaa agaaattaaa atgacagtca
    65701 acaaaatggt tgaatcaatg tataaatttt taaaagaaga atggaaaaaa cctgctttta
    65761 ttgaagtgaa acagatacgc aataaaacat cagagcatat tgacacccag atgcttgctg
    65821 atgaaataac aacaaacctt attaaacgcc gtataaaatt cattgaccag tcattgactg
    65881 ctgatgctat cgccgaaatg gaaaaaggta tgactggtat ggttgacccg gaaagtgcta
    65941 tccccgttgg cgaattaaaa tcacccaaca tgtatttaac cggtgaagta cgagaaaatg
    66001 ttcgtacagt gggaagtaaa tcagtccaat accttgtagt aaccctcaaa ctcataaact
    66061 tgaaaacaca ggtagtggaa tggcaggatc agcaggaatt tttaaaatca gccagcaaaa
    66121 agaagataac attctaattc tgaggaatgt atatgattca aaaaattaaa tccctgtttg
    66181 ttgtaatgtt acttatagga gttgctgcat gcgccagtta cgattataac aaacagattg
    66241 cagaagttga aaaaatgttt tatgaggggc aatacaaaga agcagctcgc aaattgctcc
    66301 ctatggtaaa caaaaaatca aaagatcagc ttctttacat gatggaatgt ggtttaatgt
    66361 tgcaggttgc cggagactat gaaaacagca caaaagtact gaccgaagca gctaaaattg
    66421 gtgatcagat aacagtaagc ttgagcaaac aagcagcgtc attacttctc aacgaaacaa
    66481 ccactaatta caatggtgag gactttgaac gagttcttat ccacatgtac cttggcataa
    66541 actatcttat gctctcaaaa cccgatgaag cccgcgttga gtttaaaaaa gtcaatgaca
    66601 tgctgcgtga tataaatgta acaaccggta aatcctataa gcagaattta atggcaaaat
    66661 acctgactgc tattgcattt gaactgagtg cagatgttga taatgacatg aatgaccgtg
    66721 aatttgcata tatcgagtac aagcaaattt atgaactcaa tcctgcatta tcaatggtat
    66781 accgcgatct tcaaaggctt gcaaaacagt taggggatac ggaagattat aatagttgga
    66841 tagggaaatt tggcaaacag gatgcaatcc ctaataatgc aggagagctt attgtcatag
    66901 tccagaatgg taaaggtgct atcaaacaat ctcgtggacc gcttttatct gatcaatcaa
    66961 tgaaggcaac tattcagata actcttaatg ggatgtcttt agctgaaggg gttaccgttg
    67021 ccggagtact acttgcactt cataatgctg aaaaccccat accaaaattt gtaaaacggg
    67081 gcaataatat caattatctt atcatcaatg caaatggtaa ggatgttggg cgctccatta
    67141 tgctggagga cgtagcttcc acagcagtta aaactcttga agatgactat ggtcgaattt
    67201 acgctaaagt agctgcaggt attgcagtta aagcagcagc agccgtagct gcagggtatg
    67261 cggcaaaaaa attagctgaa gaatccaaaa aaatgggtgg ttatgcaggg ctcattggca
    67321 ccgttgttgg cactggcgtt ggtgtaggtt tattatctca aatcaaaccc gacctcagat
    67381 gctggcatac attgcccgaa aactttcagc tttccagaat attcctgcca ccaggtaact
    67441 atacaatcac atttaaattc gttgactata gtggaaatat tgtgtcaaca caaaatgaaa
    67501 ccatagaaat taaatctggg aagaaagtgt tcctgaataa acgtatactc cccactaaag
    67561 gtgtatcccg gttttcgatt gacattgaat cgggcacagg caaggggtat ttaggagcaa
    67621 aaataaaatc tataactgaa gagaaagccc gtgaactcgg gctttctttt tcttctggtg
    67681 tttacgttaa tgatataatt cccgaaagtc ctgctgctca atcaggtata aagataaatg
    67741 atgttattat agcagctgat ggtaaatcaa ttcagaaacc ccaggattta gttcagatag
    67801 ttagaaactc tcaatcggga aaaacattgc agttaactgt cattcgcaat aaatcccaaa
    67861 tgacaattcc tgttattctt ggcagtttac cagagaaagc atataatgcc accatcccat
    67921 ccaatccagg acacaatagt tcagaagcac ccaccacaaa ctccccggag taaatttatt
    67981 ttttatattt ttattgcaaa aaaatatacc atccatgaga tggttatgta gtaatggtgt
    68041 gttctatgca aaataaccat aatagcatgg ttcccataaa tgatatgaca attcactcag
    68101 ccactgaaat ggttttaatg tttcagttac cagcatgtgt tataagtgtt tcaggcgaag
    68161 ttatcgcttc aaatgacgct ttctctgatc tggtgaatgt caaaaacctg tctcttgatg
    68221 ttacccatcc tttttatcca gaatatcgca aacaactggc aacttcatat ttgaaggcac
    68281 taaaaggctt atccagacaa tgttttgcag tttttcgtaa taaagataac cagcgtgttt
    68341 caactgaaat ttatattttt ccggtatttc aggacaccgc aattgttgct ctttttgtac
    68401 tgttacagat agttgatgaa cgaatactgt cttttgataa atcaaccgac accattatca
    68461 gccaggaaag taagaattac gaatcattgc tttatgaata ttcaccattt cccattttgc
    68521 agattgatca atcaggcaat attgttagag ccagcccctc ggtacaggaa ctcattggct
    68581 atgaaataga agatttgaag aagaatagaa atcttctcta tcgcgctatg aatcactatg
    68641 attttaacag aatgcgctta acattatcgc aaatatttga agggagtatt ggttacaaac
    68701 ggattggtga aatacgcttt ataacaaaag acaaagatga aagatgggtt aatgcaacct
    68761 gttatcctgt tcacattcaa ggtacacccc caattgtaga gattattctg gaagatatta
    68821 ccagaataaa gcgtatggaa taccagttgc aacaactcag cagattccat attattggtg
    68881 acctgacaaa aggatttctt cattcattca ataacatcat caatataatg ttaaacaaag
    68941 ctcagtggct tttacagatt acagaaaaac cttcatttca ggaaggcctg aaacttatca
    69001 ttaaatctgc agaagatggt atacaacaaa ttcgacgtat tgaagaattt attggtagag
    69061 ataaattaga agaaaaatgt gatattaatg ttgttgaagc aatagaagat gccattgaat
    69121 ttgcaaaaat ccagtttaaa gttgaagaag cacaaaagcg aagatctgta gctattgagc
    69181 gacgatattt ttcacttgtc agtatgtatg gtgatcccca catattcaga gatctgcttc
    69241 tggcaactat tttcaaagtt gcctcagtaa tagctaccaa aggtgtggtt gaagtaacac
    69301 taaaaaagaa cagtacgtta acaattgcaa taaaggcaaa aaaagaatta tttgaaacta
    69361 aagatgaatt tgcatttatt tcaactattg atctttcaaa attagctgaa caaaatcatg
    69421 caaaactaat ggaagaagaa tctttacagg attactcaat aaccattatc cttcctgaag
    69481 atactgtttc aatgcttccc cataaagaac agcaaatccc acaaataaaa ttacgtgaca
    69541 tggatatatg tattgtggaa gatgaaccag cattacagga aatcatgtat gaagtatttg
    69601 attcacttgg aaacagagta tcagttttta ataacgctga tcaggcatat gaatcattca
    69661 aagttaataa atttgatatt gttattgctg attacggatt atccggtaca acaggactgg
    69721 aattactaac taaaatcaaa gaacaaaaag aagaaacaat aaccgttctg ctgacaggat
    69781 ggattctgaa taatattaaa gcgtataaaa atgttattga tatatacatg cataagccat
    69841 ttaaattaga tgttcttatt aacgaaatct ccaatgaaat gtcaaaacgc catacatttt
    69901 gataatcaca gtatagtact cattttttcc atcatatctt tttttcctaa tgccacaatt
    69961 atatcgcccg attcaataac ctctgatgac tgagggttga aaatcatttt cccctgtgct
    70021 ttttttattg caacaattat gaccccataa tcctttctta aattgctttc tattaaactt
    70081 tttccaacca ttggtgattt tgggccaacc ttcagttcct ccatagctaa acctaactcc
    70141 gaatccatca tagcagtatc taaaaaatca actaccgtag ggcgggttaa aacctcagcc
    70201 atacgttccc cccaatcttg tatgggagaa taaccctgct tgcacctgct ctcagcagct
    70261 ttacctcaga cttttcatct gacccccggg ataatataaa aatatcatta cgcaatcccc
    70321 gggcagttaa cacaatatac acattgtcag catcagacat tactgcagtc actaatcctt
    70381 tggctttcat tatccccgcc ttcagcaagg cctcttcact tgtagcatcc attgcaacaa
    70441 aaagatagct taacttttca agttcttcaa tggcagctgg attattttca atgactacaa
    70501 atggaacctt tctatcataa agttctttgc atatcagttt gcctattctg ccataaccac
    70561 aaatgatata atggttctgt aatgattgaa tctgtcgttc caattttttc ctcccaaatt
    70621 cttttcgtaa ttcgccttca ataatcatac gtaacagtgt cccaatagca taggcactga
    70681 ttgaaacacc actcaaaatt attgtaatgg taataatacg tccataaata gttaaaggtt
    70741 taacttcctg aaaaccaaca gtactgatgg ttatcatggt catataaaat gcatcaaatg
    70801 gtttcatatc ttcaaatata taataccctg cagttccaaa tccaatagta cctagcagaa
    70861 gtattaaagc cacacgcaat tttttaaatt ccatagcaat ccttatacgt ttactaatat
    70921 aggtgttctg aagatacaat aagtgtgtaa aatgatgaac aattgtaaat aattttttaa
    70981 aaaaataaaa ttatatttga tttttattta atactatcat attttttcta aaaatataat
    71041 atatatatta taaaatagac tttcaaatgt tttattaagt aaaaaataca tttaataatg
    71101 agaggtgatg ttatggcagc atttcaacac agtacaggca catttattgg taaaggcggt
    71161 atagaaatat ttttccagca gtgggaagta gccaacccaa aagcagttct cgttatcatc
    71221 catggcctgg gagaacattc aggacgatat ggtaacctca tcgatgcttt acagaataag
    71281 ggaattgcaa tctttgggct tgaccacaga ggttttggcc gaagtggggg caagcggggt
    71341 catgttgata gttttatgga ttatatctat gacatgaaga tctttgtcaa catgatcagg
    71401 gataaacatc ccaataaacc tgttattatg ttagggcatt caatgggtgg cgtgcttgca
    71461 cttaaatatg cacttaccca tgcggaagat ctggatgcac tcattctctc ttccccaggt
    71521 cttgtcccgg ctatcaaagt ccctgcatgg aaaaagaatc tggcaatatt tctttcgtcc
    71581 agaattcctt cattaaccat gccatcaggt cttgacgcaa caactatctc ccgtgataaa
    71641 gaagttgtta aacagtattt agacgaccca cttgtacatg ataaagtaac accacggttt
    71701 tatgtagaaa tgatgaatac aattgatgaa tgtatcaatc gttgtggaga aattaaactt
    71761 ccattattac ttttccatgg tactgcagat gcactggtat tagatgaagc aagtaaaatt
    71821 gtatatcaga aggcaagttc aaaagacaaa acacttaaac tttttgaagg cttatatcat
    71881 gaaacaatga atgagcttga acctgaccgc aaaaaagtgc ttaaagttgt tgctgactgg
    71941 attgttgccc atgcaaaaaa aaccaccaca accaaaccaa aagcagcatc aaaacctgta
    72001 aaaaaagcag caccaaaaaa agcaaaacca aaaaaggtaa ttaagagcaa acctaaaact
    72061 tcaaaaaaag ctaaaaagaa atagctctat agtaaactgc acgttactat ttaaaacaaa
    72121 cacttagata tacattaatg taatataaaa agccgggtgg gagagcccgg ctttttactg
    72181 tggtgttgcg atgctatctc aggtgttatt tcagaagttg taatactgtt tgcgactttg
    72241 tgtttgcctg tgcaagcata gcagtagcag cctgggtaag tatctgattc tttgtaaatt
    72301 ctgccatctg ctctgccata tcggtatcgc gtatgcggct ttctgatgcc tggatatttt
    72361 cataggcatt cattaacccc ttggcagcat gttctaatct gttgtaatat gcacctaagt
    72421 ctgctctttg ttttgaaatt tgccgcaatg cctcatcagc taaaccaata actgcattgg
    72481 ctttccctgg tgttgatatt gatataaatg taagtgtaac agggtttcgt aatcctaaag
    72541 cagccgtagt cattgtttca atgaataccc tttcgcgctg atgcatatta ggccccatgt
    72601 ggaaccacat tgaagctgat ggatgaagcc gtgcaaaagc accggtcaat agcttcattt
    72661 tgttaaattc agcctgtgat gcaatacggt caatttcatc aacaagctct gatacttcaa
    72721 cctgaatctg ctgtctgtct tcaggtgtgt aaataccgtt tgcagactgt actgctaaaa
    72781 ctctgattct ctgcagtata tcatgtactt cctgcaaata tccttctgca gtttgaatca
    72841 ttgacatacc gtcttcagta ttgcgttcag cctgccgtaa tccctgaatc tgggaacgca
    72901 ttttttcgga cacggctaat ccagatgcat catcacctgc tttgttgatg cgcatccccg
    72961 atgagagctt ttccatgtcc ttggtgattg cccaatcgtg aaatttcagc gttcggtggg
    73021 caaaaattgc gctgatgttg tggtttataa tcatggtatc ctccttgaac gatatactat
    73081 catccgtgat agcattaact atataaatag ttcattccaa agttcgtctt attgaaaaaa
    73141 acgactgaga tagaatattg tcatttataa gcttttatag acgtttatta caatatatgt
    73201 tattatatta tagagttatt attgttttat ataatataaa aaaactttta ttaatactgc
    73261 attataaccg tatactgctt ccgtcagcac aattgctcat tatttgatat tatgttcata
    73321 aatcatagat gagaaataaa tagtggaaat tttttaaaat ctttttcttg aatataaatt
    73381 atcattgata aatagtgtat actgtaatat ttcaattgaa aagcatcaag aggttatatg
    73441 aacaaacata ctgataagat attttccctg attgaatcaa ctccctttct atcaaacatc
    73501 ataaaaaaag gtggcattca gttaaagtgg gttgtgaccg tatcaatgtt aatggtaata
    73561 gttattgcag taataagctt tttgttgata agtatatcag agacagccct gataaaagcc
    73621 aatgataaat tatgtcaaac tatcgcctat aatatatcct cttcagaaag ttttttaact
    73681 gcagaaaaga aaccattaaa gcgctccatg atattacagg acatagtgaa ggggctttca
    73741 aaaacaaaca ttgaaggatt tgtctatgct gccatttatg atactcaggg aattatagct
    73801 gaaaagaagt atacctatgc agcccatagc aatgcaaaac ttcatggcaa atactactca
    73861 aaagatcttt taaagcaatt tgaatcaata catactaccg ttaaagaaaa gattgaactg
    73921 actattgatt ctaaaccaac agtatgttac cggtatcgtg tcccctttga gttttttaaa
    73981 gctaagattg gaattattga gatagttttt actgaagatg caatccttgg cccggttcac
    74041 agagcacggc tggtaatgct cattgttgca gtcatattgt tgtccttaag catatatgcc
    74101 gcaatgaaaa ttgcacggca aatggtagaa ccaatagtgc aattgacatc aggtgttgta
    74161 cgggttaaaa atggtgacct cactacacgt cttactataa ccacacatga tgaaattggt
    74221 attttgacag aagaatttaa tgagatgatc aatcacctgc gggaaaagct caaaatggaa
    74281 aaatttgttt ccgaatcaac tatttccatg attcgtgaaa aaagagacga tgaacttgat
    74341 cttggtggaa caagaaaaaa tatggcgttt cttttctcag atatcagggg ctttacggcc
    74401 atgtcagaaa agcttccacc tgaagaagtt gtaaaaattc tcaatgaata tcttgatttg
    74461 caggcgtcca taattaagaa gcatggtggt gacattgata aatttgttgg cgatgaaata
    74521 atggcagtat ttgaagataa aaagaaagcc gatagcgccg ttgaagcagc tgttgatatc
    74581 attgaatcaa ttgaaaatct aaacaagcag cgagctaagg aaggcaaaat aactgtcact
    74641 gtgggaattg gtatccatat gggtgatatt gttcatggaa ggatgggttc aaaggagcga
    74701 atggatttta catctatagg agatgcagtc aacctggcag cacggctatg ctcccatgca
    74761 gattcgcaaa ccatcctggt atcgcaggaa attattgcca acctcaccaa aagaaaattc
    74821 aaaggtaaaa agcttgaacc tatcaaggtc aaaggcaagg aacaccccat acccattttc
    74881 cagataaccg gtaaagcctg attaatcctg atgtataaca ggataaaatt cttttctgat
    74941 aaagtagctg atctttcgta aaatttcttt atattgcttt ttattgttct taatattttc
    75001 aaacaatatt gcagcctgca ccagactatc cagatccgcc tcagttatgc cctgagtatg
    75061 cagttctgca tgttcagtgg tgttaagaat caggttatat ttttttgcaa tctcatccag
    75121 aattatgatt tcaattgaaa tatcaagatt atcgccaatg gaattaccaa ttcgcaaagc
    75181 atggtaggca aaattatttg tcaaaaaaga attggcatcc agctttttag caaccaactc
    75241 cgggacggta tgtgttgcta aataattatt caaaatttct aatcgtttca ttatactatt
    75301 cccccatcaa aaaaattata tgctatcaat cttttcccct ccctccaaac aaataacatc
    75361 gattccggga tctatttatt agtacctgta tataaattac cgctgtagta cattgtcagt
    75421 ttttataatc atcctatagg taccgtaaaa atgcaacaaa ataaattaga gtatgcttgg
    75481 tacataaggg gataatgggg gaaccattca ttgttcacat taacttttgg tgatagttac
    75541 tgatatatat cctattctaa agaccatgat tgggaaaaga agccccctta ttttttattt
    75601 gcaattttaa aacgataatt tacaatgcta ataaaatcta ataatgtata taggggtgct
    75661 tatgccaaga aaagagtaca tttcagggac tttttgcatc gacattaaat gtacacatca
    75721 caaagctctg gaacaatatt ctggtgatga gtatttcaaa caaaaagctc ttctatgcgc
    75781 ggattgttac gcatggaaat tttttacatg gcttcgcgat cataagtatc gcataataca
    75841 aacattacct gagatctctg ctcgtgaact ggctgcacgc attaaaggca ttgatcctgt
    75901 aagagttgaa gacttgactg aagacgaaat attgtgttta tagtatcaag aatataaata
    75961 cgtacatttc acagattttt caaattgcta cggaggtata caatggttcg taacataggg
    76021 atagttattg tttctatcat tattattgca attgtactgg atgcaattgt gtccatactt
    76081 tcacggcctc aagcaggcag aaaagcatgc caggatgcag gcatcccgta taaaactgtt
    76141 atagctcacc gtggagcatc gtactgggca ccagaagaaa catatttttc atacagtatt
    76201 gcaaaaaatg ttggtgccga ctacttagaa atggatgtgc aaagaactaa aaatgatgtt
    76261 attattgtat ttcatgatga cacgttaaat cggataacca atgttgccga agtttttccc
    76321 ggaagagaaa atgatccaat caacagtttt actatagcgg agctgaaaag gttggatgtg
    76381 ggaaaatggt ttaacacatc attccccgag cgatccaggc attcatacag tgggacacaa
    76441 atacttacgc ttgatgaagt tatagatata gctacatccg ataaaggatt atacccctta
    76501 tatattgaaa caaaaagccc caaaaattac cctggcattg aagaacaact tgtccatata
    76561 cttcaaaccc gcggatacat agaaaatgaa aacaaaatta atgccccata tgcaccagta
    76621 atattccaat catttgaaat tgacagtttg ctaaagcttc aaaagttagc accacaggtt
    76681 ccccgtatat atctgattga tgaagatatg gttaaccaga aaggatggga tacccttatc
    76741 gctgaagcaa aacaggcaca atgtgctggc atcggaccat caggatattt aggctggcca
    76801 tggataactg gcaaagccca ccgtaatggg cttcttgttc atatatatac cgtgaataaa
    76861 aaatggcagt atcgtctttt agactggttt ggtgcagatg gattttttac tgacagatgt
    76921 gaattgttga tggaatatta tgtaaagccc cataaaaaac catttaagga gattttagca
    76981 aatattgatt aaattggtat tcatagtatt aaaggtagtt tctgtcaaaa accttttttt
    77041 caggttaatt ataacttttg attttcacag tttaaacgtg cttgaaccat acatgcatat
    77101 gcactttatt aacaggaaaa tggcgatagt acatatacaa aaagctatat gctgcacatt
    77161 aaatgaccga tatgtaaagt aatgggtcac attacatttt acttgactat caatatagta
    77221 tagtataaat ttaaaaaata caattttatt tttatcttct aaacaggaga tgtttaatga
    77281 gtgaatggtc aatagctggg aaagggatat taactccgga taagttcgtc aagaaagcaa
    77341 gtatactggt cagcgatggt ataatcgata aaattgatgg ccgtagtagt ttttctattg
    77401 aagttgatgg catcattgtt cctggcctca taaacagcca tgaccattta ctgggcactt
    77461 accaccctaa agttggcaat gggccctatg aaaactggct cccctgggat aatgacctta
    77521 aaagttctcc aatatatgaa gagcgtcagc aaatagaaaa ccgtgacctt tatcttttgg
    77581 gtggctatcg caatcttttg tcaggagtaa caactgtttc agaccatata ccccattttg
    77641 tcaatgagcc atttattgac atactgccgg ttaaagcact gaaaaattat gcgctggctc
    77701 atgcggttgt ttcttttgct ttggcctggg gagatggcat aacactggaa taccagaagg
    77761 cagctaaaga gcatataccc ttcattaccc actcaggtga aggttttgat gatgaaaccc
    77821 gtcatgatat tgatacattg gaacgtttag gagcgttggg tgaatatacc gtactcattc
    77881 atggaatagc tttcaatgaa caagatatca agcgtataaa aaaagctggt gcatctgttg
    77941 tgtggtgcgg agattccaat atgtatatgt tcaatcgtac agcaaatgtc aagcttatgc
    78001 tggaacatgg tgtcaatgtt tgtataggca ctgattctcc catgtccggt ggcgaaaatc
    78061 ttttatatga aataaaattt gatcgtaaat tatacaaaaa gctttttggt caggaactca
    78121 gtgatgaaac aatagtaaaa atgatcacgg taaatccctc ctatgcgttt aaattacatg
    78181 accatggggc aataaagccg ggcaatgttg cagactttat tgtgatttcc cgtatggaag
    78241 ataatcctta ttcagcagtc gtaaacgctc agcttgaaga tattgcactg gtggttatta
    78301 atggaaaacc aatgtatggc gatagttcct ataaaaaact ttttgaccac aataaagtca
    78361 aatatcagga aataatcctg cgaaacaggc acaagctggt tgttggcgat ttactagggt
    78421 tactgagaag aataagcaaa gcagttggtt ataagaaaga atttccattt attcctgttg
    78481 aatttgatat ataacgtgtg cgaggatttc tatgtcaaat ggttcaccaa ctcaagggat
    78541 taccacacga acctataaac caggctctat tgtttatttt gaaaatgaca aaagtgaaaa
    78601 tatttatatc cttaaatcag gcctaataat tttaacttct caaaagctgg aaaccggaga
    78661 ggaagtcaaa gaagaagtaa gacctggtga attttttggt gtaaaatcag cactggggcg
    78721 ttacccgcgt gaagaaactg cacaggtgat taaagaatca gtggtgctag ttttgacact
    78781 tgctgatttt gaacgtctca ttttaaagaa tgtcaatgtg gttttaaaaa tgctccggat
    78841 cttcagcaat cagctcagac gaattgggcg cgcagtacgt gaagtattag gagaaacaag
    78901 cactattaat cctgaggagg agctttttaa aattggtgaa tattattata gagctggccg
    78961 ctttaatcag gcacggtacg catataataa atatcttgat tactatccaa acgcaaaata
    79021 ctctgattta gcaactaagc gaatacgcga aattgattcc ggccaggctg aatcaaatat
    79081 tgcacctcaa ccagtaaaac gtaaggaggt ggaaccttcc acagaggaat taaaagattt
    79141 taccctggac gaggatatcg attcaatgcc ggcaaaagaa tcagtagtgg atgaaatgga
    79201 cgatttttta ggtgaaaatg attctgcaag ttcagatttt gacattgccg atgaaagccc
    79261 aaaacacgaa gatatatcct ccatgtttta tgatgctatg agtctgtatt cacaatccga
    79321 atatgaatct gcattacaaa tttataaaaa aatcttaaac cagaagtcat taaaaaatga
    79381 tgcggaaaat aaaatttatc aaaaagccca ctttgaaata ggacgaacat tttttaaaat
    79441 gggtcaatac aatgaagcca tcgaagcttt ttctttgtta atgaaaaaat tcccacaatc
    79501 tgaattaaat gttaatgcat tataccattt agctatgatt tatgaagtaa agaaacaatt
    79561 tgataaagct aaatcatatt atcagaaagt gatatctctg gcagccaagg aggaaatagg
    79621 taaacaggct tcacggcgat taaaaaatct tgaatcacgg taaaggtatg ctatgttact
    79681 tgatgaaaag ctatttgaca aatttggcgt agaatatgag cccaacgata taattttctg
    79741 cgaatttgaa cctggcaatg acttttactt tattcaatca ggaagagttg agattgtaaa
    79801 aatcatcaac aaccgggaaa aaactcttga tatactcggc cctggggatg tatttggcga
    79861 aatggcaata cttgaggaag aaccacgttc cgcatcagct atcgcaatag aacatgtaaa
    79921 gttactgcgt tttcgaaggg aaaattttga tgctctatta cagggtaatc ctcagcttgc
    79981 atataaactg ctggttatct tttcaaaaag aatttatgac gcaaaacgga ggcttatgat
    80041 tttgttgctt gatgacccgc agttgaaagt catggatgta atgaatatgt tagctgaaca
    80101 ggatcctcat ttagatgttc aggagccagt tacgctgcgt gtaaccgtcc aggaaattgc
    80161 taactgggct ggaatgcaaa taaatgatgt gcaaaaaata ttgacacatc ttaataaatt
    80221 ggggaaaatt gaattattca atgatagagt agttgtcagg aatatcaggg acttccagcg
    80281 gctggtaaat tcacgccgta aaagtatgta tgcataagct cccgtagctc aggaggatag
    80341 agcagtagtt tcctaaacta tgtgtcagag gttcgagtcc tctcgggagc aaagagttat
    80401 aatacctata ctagcgggct atcccgctgt tttttttcaa gaggcattga ctatggaaga
    80461 acgtcttcag caaataatta accgctacaa cgaaattgaa catgctatgg ctgacccaaa
    80521 aactgtcagt gaccaaagtt tattcaaatc actgacaaaa gaacatgcca ggcttacacc
    80581 agtagttcaa aaatattatg aatatatacg tataaagaaa gaaattgatg atactaaaac
    80641 tgccttaaaa tcagaaaaag accaggaaat gatatcctta cttgaggagg aaatccctaa
    80701 acttgaatct aaacttcatg aaatcgaaga gcaaattact caaatgctat taccccagga
    80761 ccctcatgaa ggcaaagata tcattattga aatcagggct ggtacaggtg gagatgaagc
    80821 ctcattgttt gtagcggatc tatacagaat gtatatccgg tatgctgacc tgaaagggtg
    80881 gaaggttgaa cacatagacg ccaatcagac agaattaggt ggctttaaag aagtaatttt
    80941 ttctattagt ggagctgagg catacagctg tttaaaattt gaatccggtg ttcacagggt
    81001 tcagcgaata cctgttactg aatcgggtgg cagaattcat acatctgctg ttaccgttgc
    81061 agttcttccc gaagtggagg aaagtgaagt tgatatcaat cccgacgata tacgaattga
    81121 tgtttaccgt tcatctggcc atggtgggca atcagtcaat acaactgatt ctgcagtccg
    81181 tataacccat atcccaactg gtattgtagt aacatgccag gatgagaaat cacaacataa
    81241 aaaccgtgct aaagctttgc gggttttgcg cgccaggctt tttgaaaaaa tggaacggga
    81301 acgaatggaa aaagaaatag cattgcgacg atctcaaata ggcagtggtg accgcagtga
    81361 gcgcattaga acctataact tcccccagtc acgggttact gatcatagaa taaatctgac
    81421 catttatgat ctggaatctg ttcttgacgg tgaactggat aaaattatta tcccattgcg
    81481 ggaaaatgaa atggaaaaat tattaaaagc ttctcatgtc tgatgttttt tataataatt
    81541 ctattatcca gattatagct tttgcctcaa aacgtcttga atccatttca gcaacaccag
    81601 tattagatgc tgaaattcta atggcacatg cattgggtat tgaccgccat aaactggttt
    81661 tataccataa tacgttagtt gacaaaaaaa ctttaacaaa atttaaacgg ctactttcac
    81721 gcagattaaa aagtgaaccc attgcctata tcgtaggtta caaggaattt tactcattgc
    81781 cattgaaggt taacaggcat gttctcatac cacgtcctga aacagaactt ctagttgatt
    81841 gcgcaattta ccatgccaga caacgtgcaa ccattttgga tatagggaca ggaagtggtg
    81901 caatagctat tgccattaaa tataacagac ctgactgctc tgtatgggca acagatattt
    81961 caaaggatgc tcttaaagta gctaaacata atgctggcac aatccttgga aaacattcaa
    82021 taaagttttt acatagtaat ctgtttgagt caataccaac aatgacattt gatgtaattg
    82081 taagcaatcc accatatatc aaccccaata tatatactca gttgcaaaag gatatccatt
    82141 acgaaccaaa ggaagccttg cttgcacctg ataatggatt tgggattatt aaatcactaa
    82201 ttgaaaaagc aaataattat ctttctgata acggcactat tattatagaa attggagaag
    82261 atttatcatc aagaatacaa aattttgccc cactgtttaa cttctctatt tcaatcctca
    82321 aagattattc aggattacct cgtattgcta tatttaaaaa aataaaaaat atcaacatat
    82381 aagaaattat gataaattta taataatttt attcgatatt actaataact gaataatggt
    82441 tcaagcaccc agacattgac atttgattat tctggtccaa acgagcattc cagcaaaaag
    82501 gggaagcaca ctgcatgatt aataccagag aggaattgtt ccatgatagt aatttaccag
    82561 attttgtgga atcatacagg ataagtgtta tcagtaagcg ggataaatgg tatagctttt
    82621 caaatataac atggaatcct catattcgtg aaattgggat ttattcatta atcaactttt
    82681 ctgacttttt tgtaacaggc agccagttta taaaagatac tgtaccaaaa aaatcttttg
    82741 gatctcagcg gagcaaatat actattatcc agccattaaa ggagagcaag cttgtttata
    82801 aagattctaa tcttagtgcc agtatatctt tattagatta cttcccctat tatgaattgc
    82861 tgcataataa aaaaccttca atagaaagtt ttgaaaatat gatttccagc tactttaccc
    82921 gttgcaaggc aaaagtatct gttgaggtgc acaccaaatc aaaaattatt aaaaaaaatt
    82981 ttgattgtat tggattcaag gaacacacat ggggtttata cccccacacc caaataaatt
    83041 gtgatagtag agttgttgtc catttccgtg accagaccat tgcattccga tacattgaat
    83101 ataatgggtc gagctattct tatggttgta tctcacgaaa aaatggtaat ctggcacttg
    83161 tattgattga acttgaatat ttatcaataa aaaacgcaca gttggaatca tctgaattta
    83221 cctataaaga tgctcaggat gatattgatt taattgtttc aaaaccaatt aaaactcaag
    83281 taattgatgt tccaaaaaaa gctaaaaaat ctcatattca tgttgtaagt ttttcagatt
    83341 ttaccgtcat aggcactaac aaaaaaggat atggaataga agaacattat atttcttatg
    83401 accgcttaaa aaatactcta taatactctt tatgataata acatctgaac aactcaagcg
    83461 catgggactt tttataatac ttctaataat tctattagtg tataatttgt attcaaaatt
    83521 atattataac tggtatggta tagatatcat tgtcagatcc tattcttttc ttttttcctt
    83581 tttatgtatt ttcaattata cccaaataga attaaaaagc tatgaaactg tatatcgcaa
    83641 ccgtttccct aagtttgcaa accttattat ttttttggaa tcaagggtta tacctttttt
    83701 acttatatat tttatttcaa cccttcatat attaatagac aatatcaata acagtagctg
    83761 gccatatact gcttatattg ggatactgga tgggcgatat accaatatta ttttttactc
    83821 attaattctt tttggtgtac ttcggtataa gataaaacct actattgcaa taccattatt
    83881 tattggggga tctatagcgt tttattttgt ggataaattg atttatagca acatcatagc
    83941 tggtactgca atagtattta ttaaactttt aaagctcact gtacttacag gggtactggt
    84001 gtttgagtat tttcatgtaa atattcctca acttcttgta attgctatag caaccggggg
    84061 tatacttttt tcaggatcta ttggaactta tatagttttg tattctactg tcaaacatgt
    84121 acctattaaa aaagaaatac aattcaaact gcttcgttat ggaataccat ttagaattaa
    84181 tgaactcaaa cagtatgttt tgacaaaacg tcaatataag gattatcagt tgttcatcat
    84241 gtattcttca gcattggatt tacctgttaa ttttgctgat gaagaatgga gcaatctcct
    84301 tttttcggaa aatatacaga tggcagatga ggttgcttca gtactattaa agaaaagcat
    84361 cactatacaa tttaaatcaa ttattgatta cgcatatact atatcattaa aacaaaatga
    84421 acaattacaa tctgccagtc acctggcgcg tctggctgcg cgatttgctg atggtaatga
    84481 aaaaacgatt atatcaatgt ttgagaaagg gaatatttca ttaaaaatct ggataatgag
    84541 tgtaatggga tttcacaaaa aaattatata tctgcctgct ttgctaccat actgtgcaga
    84601 cacaaacaaa acattagcta cctctgctta cagcactctg gtacagataa caggtaaaaa
    84661 tccttctgct gaattcaact tgccaaccaa tgacccacag gtcctaaaag ctttcaaaga
    84721 tttttatttt caaagtggca atacaaaata atggggcatc tgctgcatgc tggctttcgt
    84781 gcatggcata ttgtccttcc atgatagatg aatagtaaat gtgctataat ccagtctgat
    84841 ttcctgatgc tttccatcat cgccatttca atagcaatag gttttctgct atttgcatac
    84901 cctattctac tggcaacacg ggatacatgg gtatcaacgg gtattgcagg tatgccaaaa
    84961 ccaaaagcca atatcacatt ggcagatttt ctgcccaccc ccggcaggct cattagttct
    85021 tcacgtgttt caggtacaac tccattaaac gtatgtacaa tagttccggc caatgcaaca
    85081 atatgttttg ccttgttgtg atagaaacct gttgatttaa taatttcttc aacatcatcg
    85141 atgtgagcct ttgccagtga ataaaaatca ggatattttt taaatagtaa tggagttacc
    85201 ctgttaacct gattgtcagt tgtctgagca gacaacacca ctgcaataca caactgatat
    85261 agattgttaa agtgcaatgg tgcagaaacc tgatactgct tcttcaaacg attaatgatt
    85321 ttttttgagg tagctttatc tatcatgagc tatcgctata agaagtgatt gagcctcttt
    85381 ttgcctggac ttgaatacat gtatatctgc aatatcatca ttagtaaaat aatatccaca
    85441 gataagtggt tcatcatatt tcatgtaaat aatagcttcc agatcttgtt ttgcctttgc
    85501 atgtgcaccc tggcttaaca atagttttgc cctgtaataa tacgcttcaa agtgatgggg
    85561 gtttttttca atagcctgtg taaaataatc catagctttt ttactttcat tcataaaata
    85621 atatgatagt ccagccccaa acaggatatc ataatcgatg tagtttttct gtataagccg
    85681 tgtaaaatcc agtgcagccc attcatatcg gcgcttatat acattttccc aggcattgat
    85741 aattgtctga tttatatacg ggtcattaaa ccgccagagg cttttatgct taccgtattg
    85801 tacggatgaa cagtagtaaa gcaggatacc tgtaaggcat attataaata gtaaacattt
    85861 tttcatattg ttttttcacc tacaatcttt tctaacagga ttttaacctt ttgtaaagca
    85921 cgtgctctat ggcttattgc attttttacg tccagaggta attgcgccat agtctttcct
    85981 aattctggta tatagaaaac gggatcatat ccaaaaccat tttcacctgc tggttctttt
    86041 gcaataacac cttcacatac accttcttct atatattctc tgccatcggg gaaaaccaga
    86101 gccataacac atacaaagcg tgcctgtcgc ctgtcatcag gaacagtatg cattttttgt
    86161 aataatagca tgtacctctc tttatctgtt ttaagattac caaatcttgc tgaatatact
    86221 cccggctcac cattcagtgc atccactaca agccctgaat catccgcaag agcaggcaat
    86281 cctgtactat tgcatacatg tcgtgccttt aataatgcat tatcagtaaa ggttgagccc
    86341 gtttcttcta tttcacctat ttcgggatat ttttcaagtg aggtgaatat tattgaagga
    86401 ataccaaaag aaagtatttc ttttattttt cctttattgc gcgttgcaac cactatctcc
    86461 atatttgtta aatactccta caaaaactat tcaatgactt tgttctttac ataaaaatta
    86521 ttgattatcc attaaatgct agactaaaat agtcaatcga aaatattggt gtctgttttt
    86581 ttatttattt tcattgaaaa atttattatc attatatata gttcaaagcc atggaagacc
    86641 agaaacagtt gaatatttta aggataggta ttgcaaatct tcccgaactg gaagagctgg
    86701 taaagtcttt tcgcctgatg aatcagtatt caaagcgacg acgttttgta gtttcacgag
    86761 aggatttaaa agatatctat ggtaacatta ttgttgaaaa agcccacgat ataaacatat
    86821 cagttgtaaa attactgcaa cgaaatttca aacctgatac agactttaaa atattcagtt
    86881 ctgatgaagg cattgctatt gtaaccaata ctgaatcgcc caatgcaaaa gaattctctt
    86941 cacaacttat ctctaccatt gaaggaattg ggggaggaat ctataaacca tttattgaca
    87001 ccgtaccagg cttctatgaa cttttcaagt tgtttgaaaa aggtctttcg ccaaaattag
    87061 ttgtggtggg ctatataccg gtaaaacagg ttgcgcagga agtatccatg tataatgaaa
    87121 taaaaaaata tgacccctat atcagaatac ttgacattac ccatgatgaa ttaaaaccta
    87181 aatcattttt aaaaggggct gtaacctttc attttgacaa tgcccataaa gtatggcaaa
    87241 gatttttaac aaagctaata caggaatata ctaagccata tttcatggaa caaatcacaa
    87301 aataagcaat gatggtatga aagataagct tttacaattt tttgctataa ccctgtttac
    87361 aatcgttatt gcagttacaa caagcattac catatcaatg tactttgtaa acgcgcattc
    87421 atgggttttc tatttaagta ttgtctgcac tatgttactt attgttcttt ttgtctatat
    87481 caggataata cacaaaattc tatttataga taaaattgaa aaattacatg caattgctga
    87541 aaattttaaa aagggcgagt tccatcacaa agttggccaa atacccggcg atgatccttt
    87601 tgcaaaaata tatacagagc tgttatcggc ttccaggaat ctggaaaaca ttattaaatc
    87661 ccagaaaagc gaaatagaga agttacgtga actgtatacc aacatgatgt tttccatgaa
    87721 tgcctatatt gttgtacttg atatacacaa taccatcctt tttaccaacg acttattttt
    87781 aaagaaattt caatacacgc ctcaggaagt atcggggaaa aatattactg aactttttta
    87841 ttttttaacg ggtacagtgc tgcaggccat tgaagagata aaaaaaacgc atgaatccat
    87901 agttttggaa aaagcccacc tgatttcaaa taaccgtatt actgttattg ctgacattaa
    87961 attgtcatat atgatttcca ataatgagcc gcagataatt atggtaatgg atgatgttac
    88021 cagtaaaaca aagcgagatt atcagatttc acttattacc aggctatcag aaaaaattca
    88081 gaaagatgat gtgctggatc atgtattacg gaccatatta actgccgtaa catcagggac
    88141 ggggcttgga tttaatcgtg caatgctttt tttgaaagat gagaagaata atgtcctcta
    88201 tggtagaatg gcagttggtc ctgataccct tgaagaagca tttgccatct ggagcagttt
    88261 aagcccacaa aaggacaagg aaattttaag ccgtggtgta aatggcgatg atgcccatgg
    88321 tcaattattg aaagaaaaag ttacatctac gtcatttgat ttgagtgaag aaacaatttt
    88381 tactgaagca tttaaaaccg gcgaaacaat tcatgtatat gatgcatacc atgatccccg
    88441 tgtcaatgat gaaatcagat ctttcattga tgttaacgaa tttgtaattg ttccgcttat
    88501 tgccctgaat aaaactatag gcattgttat agccgacaat aaatttaatc agatgccaat
    88561 cactcaggat agtgttgacc ttttaacggt atttgcgtat caggcagcgt tatcaataga
    88621 aagctataac agcttagacc ttcttaaaaa agaaatggaa aaaataaagc agcgccagga
    88681 ggcaattgtt gaatctgaaa aactggcagc tattggtcgt attgcatcac acatagcaca
    88741 tgaaattcgc aaccccctgg tgactgttgg tggatattca cgcaggatat tgcagaatat
    88801 aacccagaac ccacctgatg tacagaaaat aaaaaaagca gcacagatca taatgaccga
    88861 aacagagcgc cttgaaaaaa ttcttgccaa tgtaatggac tttacacgtc cctccccgtt
    88921 cctgctaaag ttcaataata tcaatgatat aatcagagac acggcatccc tgctttccaa
    88981 tcttttccag gaaaggcgcg tggagctatc gctacagctt tatgaagtac cattaatcaa
    89041 atcagatttt aatcagatga agcaggttat gctaaacctt ttgcaaaatg cccttgatgc
    89101 aaccccaccg tatggtgtag ttgaagttaa gacatatgca gatgaccgat atgtctatat
    89161 tgatgtcagc gataccggaa caggaataga tgaggaaaac attaacatca tctttgagcc
    89221 tttttataca accaagacaa ccggtgtggg gttgggactt gctatagtaa aaaaaattgt
    89281 agccgaccat aatggtgaaa taaaagtaac taacagggaa cgaggagcag tgtttaccat
    89341 ttcaatacca ttgccataag gtatttttta tagatccatt tgtgataaag gtaaacagtt
    89401 cattaatcat atgcatataa aaaatgtttt tgatttttta tataaatagt gtacattaca
    89461 tagtagaaaa acaccaggag gttgctatga agaaaatact tattgttgaa gatgaacagc
    89521 atcagcgaga actgtatgca atggagcttc aggaagaagg atatgaagtt gatcaggcag
    89581 caaatggcaa ggaagcggtt gataaagtca aagtaaataa atatgattgt gtggtacttg
    89641 atatacgcat gcctgagatg gatggcatag aagccttagg gaagattctt tcgcgtgata
    89701 aaaaagtgcc catcattatt tataccgcat attccaacta taaaagcaac ttcatgacgt
    89761 ggacagcaga tgcatacatt accaaatcat caaatttaaa agaactgaaa gacaaaatca
    89821 aagaactggt atcagcccga agctaatgga actgcagcag gcactaaaaa aaacacttac
    89881 attcatcctt tccggtgggc agggtgaacg gttgtacccg ttgacaaaag acaggtcaaa
    89941 gccttccgtg cattttgcgg gaacctaccg tattattgat ttttcattaa gcaattgtct
    90001 caattcagac ttaagccgta ttatagtact cacccagtac aaatcactgt ctcttgacag
    90061 acacataaaa acagcatgga atctgtatta cagggaatcc ggtaatttta ttgatgtggt
    90121 accaccacag cagcggtatg acgaaaactg gtatctgggc acagcagatg ccatattcca
    90181 gaatatctac atcattgaac atgaaaagcc tcactataca ctaatcctct cgggcgatca
    90241 tatctacaaa atggattatc gtaagatgct gaaatatcat attgaaaaag atgcagatat
    90301 gacgattgct gcatgcgaaa tgccacttga agaagcccag cgctttgggg ttctggtaac
    90361 cgatagctca ttacaggtaa ctggctttga ggaaaaacct caaaacccta aaccagttcc
    90421 tggcaaaact gacgttgcat acgtttccat gggaatttat atatttacta ccgaaaaatt
    90481 agttcgttat gtttctgacg atcataagct cgatagctcc aaggattttg gcaaagatat
    90541 tattccccgc attttttcta cagagcgtgt ctttgcgtac gactacgtac agaatgaagg
    90601 agaacgcgcc tactggcgcg atatcggaac tatcgcctca tattatgaag cccacaggga
    90661 tattatttca gtaaccccta tccttaacct gtacgataaa gaatggccta tacgtaccta
    90721 catggaacaa tggccaccgg caaaatttat tcataatgaa gaaggaagaa gaggtgttgc
    90781 aataaactca ttggttgcca atggtgcaat cataagcggg ggttacgtgg aaaactcggt
    90841 catatctcca ggagtgaaag ttaacagttt ctcagaggta aagggcagta tcattatgca
    90901 tggttctact gttggacggt atgcaaaaat aaaaaacgct atcatagaca aagataatca
    90961 tataccagaa cacatgcata ttggatacga tctggaagaa gataaaaaac attttaccgt
    91021 tagccctgaa ggcattgtgg taatacctaa aggctatctg caataaaatt atagcaaaca
    91081 acattttccg tgatagatta attgaatcgt gatgaattgt tagttgggga ggggaattct
    91141 gtggattgta attttatttt ttgcctgcat aaccatcagc ctgttggtaa ctttgatcat
    91201 gtgtttgaat gggcttacaa tgattgctac cggaaaactc tggatctttt atatcaatac
    91261 cctgagttta aatttgcaat tcacaacact ggcccccttc ttgaatggat tgaacgtcat
    91321 gacccgacat actgcgacat attagcacag atggtgcagc gaggccaggt tgagattatt
    91381 ggtggtggtt tttatgaacc aattttttca attatcagtg aagacgatat ccatggacag
    91441 ctgtccttaa tgcagagctt ctgtaaaaag cgatttggca cagaacctga agggttctgg
    91501 acagctgagc gtgtatggga tccggatata cctcaactgg tatatgactt taaattacag
    91561 tatacgctgc ttgatgacat ccattttcgc tatgcaggaa tacccgaaag tggattgtat
    91621 gggtattata taaccgaacg gctgctgagg cccttacgcg ttttccctat agataaattt
    91681 ttgcgctatt ccatcccatt taaattgccc catgaaacaa tagactattt caaacgccag
    91741 tgtgataaat acggtaactg cgcctttgtt tatggtgacg atggtgaaaa gtttggtgta
    91801 tggccccaca cctatcaatg ggtatttgaa gaaaaatggc tgcagaattt catagaagca
    91861 attttaaagg aagacttcat tacattggta catccaaaag attacattaa aaagcacccg
    91921 ccaattgaca gggtatacct cacacaggga agttactatg aaatgtcgga atgggcactc
    91981 cctgccgaaa gcgctgctga acttgtcagg atacacaatg aaataaaatc tgccggtagg
    92041 gaagcagagt tttatccgtt tttgaagggt ggtgtgtgga ataatttttt aaacaaatac
    92101 catgaatcaa atgccattaa caaacgcacg ctgtatcttt cacgagaaat aaccagtctg
    92161 gagatgaaaa caggcaagga tctgtcacag gcaaaaatag aattgtataa agctcagtgc
    92221 aactgcgcat actggcatgg gctctttggc ggtatctatc tcacacactt aagaggcgct
    92281 ctgtatgaac acatccttga aagcgaagag cttgtatgca aagcaaaaaa gctacacacc
    92341 ccggagatag ttaccggtga tttccgcaat gaaggcaagg atcagatttt tatccgtaat
    92401 aaatcatgta gcattgttgt aaaccctgct tttggcactt caatttctga attcagcaac
    92461 cgtatcaaaa aagttaatgc ttttgatgtc atagctcgcc gcaaagaagc atatcatcat
    92521 ttgttggctg agcttaatga agatgaactt aacaatgata ctgttaaatc cattcatgat
    92581 atggtaacgg taaaagaaaa aggcctgaag cgctaccttg tgtatgacag ttcacggcga
    92641 tattcatgta aagagctttt atttacaacc atgccttctg cagaagagct tatgtttaac
    92701 acaatacact atacagattg ctcgcaatat ccctatacgt atgcaataca caataacagc
    92761 attataagcg atagttctgg caatccattg ccagcaataa caaaaattat tcatgttcat
    92821 gatactgacc ctgccatatc cgttcactat accatacgtg ggtttaatgg tattgtgggt
    92881 atagaatcta atgtcaatat gcgtgcacct cacgccaaag agtgtcacta cattgttgat
    92941 ggtttggcac aagaggaatc atatctggac agcatgggac agatgaacaa tattacccgg
    93001 tatgcaatta ccgatgatat tcgcaatgta atattcagcg tgcaggcaag cagaacagct
    93061 acattactgc gatatccgat atacactgtt tcacaatcgg attcggggtt tgagaaaaac
    93121 ttccagggaa gctgtgttat gttatgtttt gaagttgctg atactctgga actggacctg
    93181 atactgtcac tggaataatt atttacatgt tacagtatat gacgatcttc tgaattatgt
    93241 aaaaacacat taatactcaa tgtatctacg gtgttttaag aattattaac atatattacc
    93301 tttaattttc tttttcttct ttagccattt cctgcaacac tgctttctga acctgctgat
    93361 aatgcctggg agaatgtaca accagcgtag taaagatata ctcatcttta tgctgcataa
    93421 gtgcgtttac atgggatacc aaatctttga tctcaaaatc agaacggaca atttttcgct
    93481 gaatatcatt ggtaactatc tcctgaataa aatcataatg ggcttttaca aattctattg
    93541 catgttcttg ttcagaagtg tttgctttat actgtgctaa cgcttttaca atattatcac
    93601 tggttaaaat tgatgatatt tcatctacca ccagaatgcg ttcaggctgc ttttcttgag
    93661 aatccataca ttgttccccc ggaaataaaa attaaattat cacaacccac agaatgacat
    93721 tgcttcatcc ctcacacaga gagatggtat gtttgagcgt tgcttcattc caacattatc
    93781 atggctattg cagactatac acagtactac caataaaatt acggtaccaa aataaattgt
    93841 caaccaaaaa taagagcagt gatagtcact gcccttataa atataatcca aaattctatg
    93901 ttgtattaca gcttctgcac aaccatcata attttatttg ggtaggtttc tttaatctct
    93961 accttaaagc caatactatt tgatttacct acaagttcat ctgccaggtc agtcgttttc
    94021 cccgaaaact caacatcaat ctttgactgt ttaccagcct ggccccttac gtaaactttc
    94081 tgaatgcccc ggattctatt ttgcaaaaca tccctgaact ttgtcaggcc attgtagtcc
    94141 agtcccacta tattcagtgt aatcattcta tgagttgctg ccagtaaaaa ctttttagta
    94201 atctgattca taaacggccc ggatttaaat ttcccggttt cttcatcggt actgcctaaa
    94261 atcttcacca gagcacgctg gatagctatt ttggaagcag ttgcatcgtc tatatgagcg
    94321 cccggcatgt tcacactggt agatgccagg atacttccgg tgtacacatc aatagccttt
    94381 agattaagga ttgcctgctt tgatttcatg tttgctgaat actgcttcaa tactgctgac
    94441 tggtcagatg tcttgacatc gcctacgata atacattcag ctccaacatc atccagtagc
    94501 aattcctgaa catttccacc aacctggccc tgcattgcct gcatcatctt tgccctgtct
    94561 ttctttatca actgctgcac ggtggctgca tccacaaact caaaaccagc attctgcatg
    94621 atttccatca tggtaagttc tgttacagta aagcccggtt cattctgctg gccctcaaat
    94681 gtttcacgca ccaaaatcat aaactttggg cggccataat tttttaatgt agcttcaata
    94741 gtatcattaa cagcctgtgg gtacacctcg ccgtatatcc gtactgtata agccccttcc
    94801 tgagaacctt cttttataac cttccagttc cgtaccatcc cggtggattt tgcttcaact
    94861 atcgattcta ccagtgcata atcctcgaca attgcgcggc cttccacagt ggtacctaac
    94921 ttcattttaa ccagtttatt catagcatcg tcaattgccc tatcccgtgc cagtgcaata
    94981 tcattatcaa atattgcagc aaaaccatca gcctcgccgc acatttcaca tcctgcttta
    95041 gacgcttttt gctgtggttt ggaaccacca caggcaacaa aagaaataat cataatcaat
    95101 gctatccaaa aaacatgtcg tttcatagtt gaacctcgta ttttgaaata atttttgtag
    95161 aaagttatat atatttgata cattacatga attaattgga taagtcaaac tattttttgc
    95221 gaatatctgc ataacgtaag ttccagaata tcaccggttg ctaatcaatc attcctgagt
    95281 tactatttta tttaaaatag tgttgacaag gacttcaact attaaagtat tgttgtaaaa
    95341 tgaatatgtg caattatttt caagggtaag gagggttata catgagcctt tcaagtttat
    95401 atgacctgcc tgtcgaccag attaccaggg aatcatactt taatgttggt ggcatgcatg
    95461 gttttatgcg atggggtgta tatatctttt taattatcgc aggtgtatat attctgtaca
    95521 gtttaattca gagagtgaga atctggagaa agggtaaacc agaactaaga accgattttc
    95581 acgaaaaaag gattatcgca ctcctcaaat atgtcttttt acagaagaaa atactgaggg
    95641 aaaaatatgc aggcatcatg catgcttccc tgttctacgg atttgttggg ttgttcattg
    95701 taacgttcat catacttata caggaagacg ttactgaact attcttccat tttaaatttt
    95761 tggtgggaga tgcttatcta ttgtggtcac tggctggcga tgtgtttggc gttgctgtta
    95821 tcgttggact ggtcatggct ttctggcgca gatataaagt gaagccaagc aggcttgata
    95881 ccaagccaat cgacacattt gcactggtgc tgattgcatt tattatcctc accggttttc
    95941 ttaatgaatc actacgaatt gcaatcaccg gtttcccaac atttgaagtg tggtcaccag
    96001 tgggctatac gctggctcat ctttttggat ggatcagcat acctacactt gaggttaccc
    96061 actatattaa ctggtggctt cacatgattg gagcttttac atttattggg ctgatagcaa
    96121 ctgataaatt aggccatgtg cttattacca tgcttaatgt ctacttccag aatttagata
    96181 atgaaagccc aaaaacaaag tttgcaatgc caattatcag tccccaggag tttgaaacag
    96241 cagaatcatt tggtgtggga accgttgagc attttacctg gaaacagctt atggatggtg
    96301 atgcatgtac ccgttgtgga cgctgtcagg ataattgccc tgcatactta actgaaaagc
    96361 cactatctcc caagaagata gttaacgatg ttaaagctgc tatggatgac cgtattccaa
    96421 aattacttgt tgcccaggat ccagcatcag ttgagtccag agcattaatt ggcgagtatg
    96481 tactggaaga tgaaatctgg tcatgtacta actgtgcagc ctgtatggaa aattgcccgg
    96541 ttcagattga gcatgttcca aaaataaatg atatgcgccg ctatcaggtc ctcatggaag
    96601 gcaagatggc tcctgagttg cagacctcat tcagcaatat ggaaaacaac tccaatcctt
    96661 acggatttgc atttgcagcc cgtggtgatt gggtcactga agatttaggt gttaaaacgt
    96721 tagctgaaga tcctgatgta gacttcctct attatgttgg atgcgctgca tcatatgaca
    96781 aacgcaatca gaaagtggcg atagctcttt gcaaaatact caaggctgca ggatacaaag
    96841 ttggtatcct tggagccgaa gaagcatgtt gcggtgactc agcaatgcgt gcaggaaatg
    96901 aatatctgtt ccattccctg gcagcacaaa acttggaaac atttaaaaac tatggtgtaa
    96961 aaaagattat cactacatgt ccacacggat acaacatgct caagaaagaa tatcctaaat
    97021 ttgctaaagt aggtgttgat tcaagtggta atccattaga agccaattat gaagtatatc
    97081 atcacacaga ggttatcatg gatcttatta aattaggcaa gatccggctg tcaaaacctc
    97141 ttaatgaaac tatcacctat catgattcat gcttcttagg tcggtataat gagatatatg
    97201 atcagccacg aaatattata aaagctatac ctggcactaa ctttgttgaa atgagcagaa
    97261 accatgaacg aagcttctgt tgcggtgctg gcggtgctcg tatgtggatt gaagagcatt
    97321 tgggtacccg tattaatcag ttcagaacaa aggatgctca ggctaccggt gctacaaaga
    97381 ttggtactgc atgtccattc tgccttacca tgctttctga cggtgccaac gaacttgaca
    97441 ttcagaatct gcagacattt gatttagctg aatatgtatg ggaagcgatg gaaaagtaag
    97501 aaaatattct gaatctaaaa gtaaaaggcg tcgttgtata gcgacgcctt ttttatttaa
    97561 tcaatccgct tgaatgattg agggtactga taggtcagat caacatatac ctgcttgccg
    97621 gcattccata tatccatatg cttctgcaac acatctccta tctcttttgc tggtacacca
    97681 ccgtatactt tgtaaccggg aattttttgt ttgtttacta ccagagcatg ttcagcaata
    97741 atagcccatt cacctatctc agcattgtca cttaatgttg catgcatccc aatcacagca
    97801 ttgtccatga tgatagagcc atgtatcata accatatggc caaccgttac gcgattacca
    97861 atctgtacca gttgcatggg acgagcatga atcatacatc cttcctcaat ggcggtttca
    97921 tcaccaatta ttatggtgcc ataatcaccc cgtataattg ccccaaagcc tatatagcag
    97981 cgcttgccaa ttctgacatc accaatgatc tctgctgaag gagctatcca ggttccctca
    98041 cctattactg gtttcctgcc attaatctca tacagtggca tgtatacctc caaatattga
    98101 atataattat tgccctaaaa tacttaccgg aaccggtata aacgtaagaa taaaaataat
    98161 cacacacata taccctatac accgttccca cgtattcaat ggcctaccat caggaataaa
    98221 cggatgccct atcataagca atgtaagggt aaggataatc catactgccc atcctggcca
    98281 caatattgct aaaaagaata atatcacaaa aaagacccac ccagcatatc gttgcctttc
    98341 accaaacaat gcatagataa catggctacc atcaagttga ccaataggca taaggtttaa
    98401 attggtaacc agaaaaccaa tccagccagc ccatgcaaga ggcgaaagat gaacatcgct
    98461 accaggagga atggtaccat gaatcaattt tgtaaaaaga tagtacagca atgaatcacc
    98521 aaatactgga acgatagcat caggtgctgg ttgtggaaga ggttgtactg tagactgata
    98581 cagccctagt atagaaatta tacaacttat tataaaacca gcaatgggcc ccattgcacc
    98641 tatatataac aatgcacgct tgtctggtat tggcgattta atgcgaataa cagcccccat
    98701 tgtgcctaca atggaaggga aaggaatgaa atatggtggt gttgcaataa ccttaaaata
    98761 gcgtgcagca aaataatgcc ccatttcatg agccattaaa atccctaaaa gagaaattga
    98821 aaatggtaag ccattaataa ttatttctaa aagtgtatca cctcccgaag agccagcaat
    98881 tgatgttgtt aaaaatgttg tagcaaaaag tacaattata acaccccatt gtggtttgta
    98941 atcaggttca tagcgaggaa ccaccactct gcgttccata ttaagcgctt ctaaatagcg
    99001 tttcaataat aatcgtatcc gcactgataa tggcaattgt atatccatag ttaattattc
    99061 aataccacca gagcatcaat aacaactggc ttacttccac tgataattat tgggttgcaa
    99121 tctatttctg aaatttctgg gtgcaataat ggtacagtac tcaatatgca cagaagatta
    99181 gccaatgaag atgtatccac ttcttcttca ttcctgaatt tgcctaacag tgttttagcg
    99241 tgtatatcat agagcatctc aaatgcatcc cgttttgtaa gtggagccac tctgaacgta
    99301 atatctttca atgcttccgt gaaaattcct cctaatccaa aaagaactgc tttgccaaat
    99361 cctttctgat gtattgctcc tgccatgaat tcccgtttgc cttcaaccat agtatatact
    99421 aaaaccggag ttgcaaaccc tgcacgcttt tgtatttcat taaatgcaac ttttgcctca
    99481 ttactatctt taacaggaag aattaccaac cccttttcag tcttatgcat tatatcagga
    99541 tgacatgctt tcattacaac gggatatttc aatgaatcaa caatagcact taattctttt
    99601 ttagattcaa taagtgtttc atcaggaata gggataccat ataaggctaa cagtgttttt
    99661 gcagcatgtt catccagtgc tttttgttta ttctttaatg ctttctgaat gattgaagaa
    99721 gcctgaggat tgacatctgg taagggaacc ttttcatagg ggagccgctg tgttacttct
    99781 ttatatcgta ataatactgc cagtgctttt gctgcttttt cagggctgtc aaatacaggc
    99841 acaccttttt cctgcattgc aacagtaaaa tcatcctgat aatcaaaaaa cgatgacata
    99901 acaatcggaa taccatactt ataggggata gtacctggag tatccaaatc acttggtaaa
    99961 aaccccaaca catcttcagg tttatctaaa tgcaatgcct gctgcacatg agggaatata
   100021 cattctatga aaccttttct gaatgcacca tgaatacata gggcatcaac ctctccactc
   100081 tgtattgcaa gctctggtat tttaattgtc agcacatcta aatccaggtt aaatgtaata
   100141 tctacaggat tccctgcagg tgcatgtggt ggtatcattg gttttatttt ttcctgcaac
   100201 tgacggctga atggaggtac tgtaaaacct tctttttcac aggtgttggc cattgatgtc
   100261 cccggacctc ctgagtttgt aataatacca acacgattgc ctttaagcct tggctgtgtt
   100321 gcaagcacat gaccatgcat atacagttcc tcaacagaat acactctgat tatgccagcc
   100381 tgtttgaaga gaccctcata cagataatca gggcctgcca ttgcaccggt atgacttgaa
   100441 ccagcccgtg caccagcagt agacccgcca acatactgtg caataaccgg ctttttaggg
   100501 gtaatattcg atgcgacttc cagaaatcgc ggtacatccc gtatgccttc aatgtacagt
   100561 gaaattgttc tggtctgttc gtcattacct aagtattcaa gagcatcaat aattgagata
   100621 tctgcttcat tgccaacact cagtgccttg ctgaagcgaa tcccgtgatg tttcagatac
   100681 atgattgatt gtgttatata tgttccgctt tgtgaggcaa atcctaagga tccaggtttt
   100741 tcagtatatg ccatgactgt tgtattgaga gctatttctg aatttattaa ccccatacaa
   100801 ttaggcccca aaaaacgaat accatacttt gaagcaatag ctttaacctc ctcttctaac
   100861 ttccttcctt cttcaccaac ttctttaaag ccagcactga taatgattgc acgttttgtg
   100921 ccaattgtcc caaatgcatc aagtgttgag ggtatacctg tagctggaat tacaaatata
   100981 gcgcaatcag gagcttcagg aagatcaaga ggacttttgt atgcttttaa cccaaataca
   101041 acatcttctt ttggatgaac aggaaatatt tttcctgtat atccatcttt cataatactt
   101101 aaaaggtgca tggtagccat ttttaaggga ttgttgcttg cgcccacaat ggctattgat
   101161 gaaggattaa taagtttttc cagcggaccc ataatgatac ctcaatatgt tttttctgca
   101221 ttacgacata ctatacatat tttgcaatat aaaaatgaat gggtggtata catttttttg
   101281 ttaacttttc atacaacata taaatattgt tgaatatccc gtatgtgcag tgtactatac
   101341 tgtacaaata acttgacgaa cataaccccc aatttacgga tgtatacgat tatattcaat
   101401 atggaaataa ggaggatgta tgagtaaact tacatttgat ttgactggca aaattgcgat
   101461 agtaaccggt gcaagcagag ggattggtca ggcaatagcc caaacactgg cagcacacgg
   101521 tgctaccgtt tactgcacca gtagaaagat tgaagattta aaaaaagtac agcaggaaat
   101581 cgaatcacag ggtggcaaag cacatgccgt tgcctgccat attggcaaat tagaagatat
   101641 acagaatcta ttcaaagttg ttgaggaaca atacggtaag cttgatatac tgataaataa
   101701 tgccgcaacc aatccatttt ttggagatgt cttgcatgca acacccgatg catgggataa
   101761 aacggtggat gtaaacctaa aaggttattt ctttgcaagt cagtatgctg caaatctcat
   101821 gataaaaaat ggtggtggtt ccattgtcaa tgttgcttct ataaatggaa tacgtccagc
   101881 accttttcag ggaatatatt caattactaa agcaggcgtt attgccatga caaaatcctt
   101941 tgccaaggaa cttgcacctt ttaatgtccg tgtcaatgca atcctgccag gtcttactga
   102001 aacaaaattt tcatcagtta tgacacaaaa tcaggaaata ttaaacatga ttctgccgac
   102061 aataccaatg aagcgcgttg cttcccctca ggaaatgact ggagctgtac tatatctggt
   102121 ttctgatgct gcaagctata ctaccggatc tacaattgtc attgatggag gagcactggc
   102181 ttaaatttta ttttaatgga aaacaagggt gaacatggca attgcaacca tgaataatga
   102241 aaccgacacc atttcttttg accagatgaa aacacaggtt aaccgagcgt tggaggagtt
   102301 gtcaattaaa agcaacacca tcgtgagtga accctacatt ggttttgaaa aaggttttac
   102361 caaaatcagt gtttcaattc cggagctatc gcctgcactc ataattcagt gtttgggaat
   102421 tattaaagac catttagaac acattcgcat ccatgcattt tcggatggct attatgcagt
   102481 gcaggcttta aataaaaatc tttttaaaac cgagaatatc tttgataacc ttaaaataga
   102541 attttcgggt cttaccacaa actttaagat tgaatgcacc aaaaaaggga acctttcaca
   102601 ggaagaaata aactgcttta ttgcactggt gtacaaagcc tattcacagg caaaagaaaa
   102661 tcccgtgata cggcttataa aatatggggc atcagtctat accgatgcag gtggacttga
   102721 ctggaattat atagctggct atgaggaagt gaaacgtact atacgcgaat ccataattct
   102781 gccactcaaa aacccaaagg tgtatgacag cattgccgaa atcacccgca aaacctttga
   102841 aacaaacagg cccaaagcaa tactctttga agggcctcct ggcgtgggaa aaaccactgt
   102901 tgcccgtatc atagcaaaag aagttcaggt cccactggtg tatgtacctg tggaatcaat
   102961 catgtctaaa tggtatgggc agtcatcaca gaacttagca gctatttttg atgcgtgcga
   103021 tgatctagga ggatcaatat tattcattga tgaaatcgat tctctggcag gttcacgtga
   103081 ccagaatatg tacgaggcca cacgtcgtat tttatcggtg cttttgcggc ggttagatgg
   103141 cataggtgct atcaccaata ctatagtgat tggagccaca aatcgcaagc aggatttaga
   103201 tcatgccctc atttcacgat ttgatcagac aattcatttc ccattaccag gagagcagga
   103261 gcgtgctagc atttttggta actacgcaaa acatcttaca cctgaagagc ttgctaaact
   103321 tgcagcagcc agccactcac tttcaggcag aacaataaaa gatctgtgtg agcagaccga
   103381 acgcaggtgg gcacgaaagc ttattataaa agatctgcca ccgtctcctc cgccgtttga
   103441 gtactactat cacactgtta aaagttttat tgctgcacaa gcctgagtag tgagtctata
   103501 tgttcaaata caggatatgt ttcaaaccgt ttattgatat agctattatt agttatcttc
   103561 ttttcaatca tatccagaat tgtttgtgca gcagcagagt gttcctcaaa gcgctcacgc
   103621 gcatagtctg catagcttcc tgttgaaatc ataaactgcc agtctgaaga agaagcaagc
   103681 ataagttctc gtgcacactg ctgcaacgct tcatcattgc tgtacagcgc tttatcaaat
   103741 ctgtagagta attcttctag tttcacgaca gtatggcgca cctttggatt gtaccaggtt
   103801 gaaaaatcat ttcctcttcc ccatgacgat tccacaggtt caaatgtttc catatcacaa
   103861 tctaacagct gttgcggcat cacgcacatt atatcatcgc gggaagctat ggtctccaga
   103921 agcatttcta aaaacagcgg accttcaaac caccagtgcc caaacaactc tgcatcaaat
   103981 ggcaatacaa acacaggttt gcgtttaata acactttcaa cctcctgtgc acgctttagt
   104041 atggcatcaa taaaatcatg tacctgctgt tgcacaacgg ccatggcttt ctgcgggtta
   104101 taatattctt tattgtcgct tccgcttatc gcataaattt ttaaacccaa tagtctttgt
   104161 tcattattgg gcgataattc cggcaattca tacactgcat ccctatggaa ctctctgtag
   104221 tcagggttcc ccggataccc ctgatgcgac gaccagatct tcatcgataa aaccgcatcc
   104281 cggggaaaca aaaccagccc cgtgtgagtt ttcaggggaa aaaaatttcc tgtttttgga
   104341 atatttcgag taaacaaaac acttgcatca ttaacaatag tatagcctac tgcgttttga
   104401 tacaacagct tatcaattcc tggatggtat cctaactcgg gcagccagaa tccccgtggt
   104461 aatttcataa attgttttgc aaatgtatga attccactac tgacctgaag attaaggaat
   104521 gaaggatatg ttctatataa aggaaaaaaa gggtgtgttc ctgcagtagt tagcaattca
   104581 attgaattgt tagaaaacaa ttcaccaaaa gcatcagcgc aatttccgtt tatttcttca
   104641 aaaaatgaaa gggaatgtgt tataaacgaa ttgtaaaaat caagagcctc acacgccttt
   104701 gtattctttt ttcttagtag tgaaatggca tcaagaaggg tatgtgccca cttttgatag
   104761 cgcctgtgat agtagggttg ctgcaacatg gttagcaatg tggggcttat agatagggca
   104821 actgatggaa tgatattttt tttaatgagc ctctggcaca tctgtaaaag gggaatatag
   104881 cattcagcaa tattctgata taaccatatt tcttcaagag gtagtgttaa aagaggattt
   104941 ctgacatacg gcagatgcgc atgcagtatg atgcatagtt ttgtagtaga tttattatca
   105001 ccgatcatga agaaaggcgt ttttcgttgg ccagatcaat agtatttaat gtatgaattt
   105061 cctgagtaaa tgttgggatg gtgtctttca gattttccgg tgaaagcggc agcatgataa
   105121 catcagattc aaaaataatt tcttcacctt tcccttctat tacggtcatg agcattactt
   105181 tgatggtaca accgtgaaga ttatcaccaa tggatacaat ccagtggtta ttcctcccaa
   105241 acacatcaat ttgctggtcc agtatccact cgtttccatg caatttaaca atacgtataa
   105301 ataacaccgg atcgccatac acatcagtaa ctgcatgcga aacaatagta tcccagtact
   105361 ctttatctat tgcccagtgc acatgcatca atgaaaacga aggtaaatgt actgtaaaat
   105421 atcccgacat atttccccct tcaccaccgt gcatgatgtt cttcggccca tccaataata
   105481 ttggccacct gtattttttt accgctgtta tcaataacaa agccattgta tatgccaaat
   105541 agctgatgca cctcactcgt tataaccaga agattggatg ttgctactct ttcaaaaaaa
   105601 ggtgaaaacg ttaatcctaa ccttccatca ttggatccaa aataccatgg tttcaaaaaa
   105661 tttgaattgt cataattaaa cgtaacctta tctaacttat gaatacgccc attgacaaaa
   105721 aaacaatttt ctgtggcata tgataaatca ccaaaccctt gccctaaatt taacccaaac
   105781 ggagttccat caggtaaaaa ccaggaagcg cttgcccagt tccagaagct tttatattcc
   105841 catactcctc ttccccagtc taacgttgcc agtgcgttac cagagtcaaa tccatattcc
   105901 tgtccaccca gtaaaaatgc accacgtgct ggcatgcagt taattttttg attgtagtaa
   105961 aagcgttttt ttccaatagg tgtcatcata acgattgaat cgtgcggtgt ttgccgtatt
   106021 atccagtgtg cgtgaaaacc cttcccttta ccaaattgcg gccagctaac atatatctcg
   106081 cgttcattcc cttttttcct gaaagcaaag gttatatcat cttttttaaa gtaactatca
   106141 tcatcacttg accgcccaag tgtaaaccct ttccctaatg gcgttatgag tgtatcttca
   106201 atatactgtt ttgttttaaa atcaataaaa tacacaaaca caagtcctgc atatccaata
   106261 tgtgagatag tagctgaaac aaagtaatct ttagtggtta tgccataata gtcccattcc
   106321 tttatacgta ttttgtttaa ccatgaaaca ggatacaggg aaacattttc aggattatat
   106381 gttctgacag ggctgattga ataacctttt tctataagtt ggccatcttt accaagcaaa
   106441 tcaccttttt tagatatgag ttgttgcata gcattacctc ttttttgaaa tactatgtaa
   106501 taaaatttca ttagtcaagc aattatttgg gaagttttat acttataaaa gttgcaatta
   106561 taagcaaata tcttaaaaca aattatctag ttttaaatat cattctgata aatttcaacc
   106621 tgatgtgatg acataaacaa tttagattat tgtcttacat ttattaagac ttttcagcat
   106681 gggtatgcaa gcagtcgttt ggcaactgtt cttaaaatag aatttcatta ttatccatca
   106741 tagaatcatt catatctttg aatactttgt gtttcagaat caaatccttt atggcaaaaa
   106801 ggctgtattc aggatgactt atggcataaa tatttcatct aattgaaaat ccattcacca
   106861 cattgaataa aatagtccgt atgtacggta tactaaacat gtgtccgttt ttccatacac
   106921 ttttatagtt aaaaacgtat acatacaatg catatacgtg acattttaag attttatgca
   106981 cataatctat tctttatgcg cttctggttt ttaccttaaa gtactaaaca ttctttaaaa
   107041 ttgtatgaaa tatgcttaaa atttaaaaat ttttgtattt atataaaaaa tttgagattt
   107101 tttacttgac gctggcatac gatttgctta tatataatag tgagctggga aaatcctggt
   107161 acacaacaat ttccttccaa agtattccgc tatgcttttg tagagctaga ggtaatcgcc
   107221 cgattacctc ttttttttat tctattatta tctcatcagg aattttatat aaggaaatat
   107281 tttcactgca ccacattctg agtgtatccg ccgttaacgt tgcatggtgt tttagtttta
   107341 cacgtgcaat aagccactct ctggtgctat cgcccagaaa ctcaacatgt cctgttaccc
   107401 gtacactctc tatatcagga tgataggata gtaaccgctc cacctctttg ggatatacat
   107461 tgtatccatt tttcaatacc atatcctttt ttctcccgga tataaataaa tagccatcat
   107521 tatcaaaata cccaatatca ccggtatata gccatccatc tttaatatat tcttttgtct
   107581 tttgtggatt attatagtac cggttaatca cattcattcc ctttacaatg atctccccat
   107641 tcatgcaagc aggaacatct ctgcagctat cgtcaaccac gcgcacttta ttatagggca
   107701 ttggttttcc tatggaacca attttatttt caatttctgg catattccag cttacaatgg
   107761 gcgaagcctc agttagtcca tacccctgac gtatatccac ctgacgttca gcataccatt
   107821 tttgaaggga aactggaaga taatcgcctc cactaaccca taactgtata tgcccaaaat
   107881 tgttaccacg ctcaaagagg cctttcataa tagcatacat ggctggcact ccaacaaaag
   107941 acctgacttc attatttgac agtatccgta tcaatttttt tagtgaaaat ttttcaacca
   108001 gctcaatagg aatatttttc aaaagtggca caacaagcga agaggtaagc ccaaatgcat
   108061 gaaacaaggg aagtacagcc actgaaacca gagcatgtcc aggcatcatc tgagctactg
   108121 cttttgcact ctgataaatg ccataatggg taagtactgc acctaccgca tacccatcca
   108181 ttgcggaggt aaatattatt gctgctgcat cattgttttc ctgaacataa tggttgtcat
   108241 aagtatcgct gccaacaatt gaatccattg atgtatatgt actgctttca tcgatggtaa
   108301 tgatagtatg aatatcgctg acaataccat gctgcaatag tctctcagcc ataaagcttg
   108361 tggtaatgat acaatcagga cgcgtaacag taatatagtg atgcaattca aactgccggg
   108421 ctaaaggatt aaccagaaca ggaatggcat tgcactgcaa aatggcaaaa aagctataaa
   108481 caaaatatgg tgtattaccc atttgtataa tcaccgtagt tttgctatga actccacgct
   108541 ttcgcaatgc atgtgcaatt ttatgtactt tatgtaaggc atccttatac agtacaggca
   108601 cattattata gtgtagcatt attttctttg gaaatttgtg tgcctgttcc tggaataatt
   108661 catacagatt atacgtaaac tgttgtccac acatagttca ttatcccttt aagcgatagt
   108721 atatagtatc atgatttata ctaaaaagac aagtaaatgt ttacaggtgt tccaatgatt
   108781 ttcttgactt atgcttacca gtttgtataa tacaaaagat tataatgtat aacccccgcc
   108841 aaaggtagcg gggttataat tattcaataa ttacatacga gcatatatga ttgttctaac
   108901 atttattatt atcctgcaaa tatcactggc cgtccacgtc tattatatta taagctatat
   108961 ttcaaaaaaa caggacaaag acttcaaggg atttcttttt acttcaatga ttaatatttt
   109021 tttaggaata tttttgtcag tttttatttt attatttcca caagatctta aggcaatcaa
   109081 tttagaacgc atactcttta ttgaatctgg agtaattttt tttggcatgc tatatgtaaa
   109141 aacaaaaatt gcagtaaata tatacaaacg cacaaaagat ccccagtatt ttcattattc
   109201 atattttggc aaaaaggtac tgcatcccaa tgtggtaaaa atgcccgaac tttttaccta
   109261 ctttttaaca cttcccctta cactggtatg cggtgcatat tttatagtaa aattaggatg
   109321 tgggaaataa tatatcagtg ttttacattc tgaataattg acaccaccaa cgatgcaaca
   109381 ttaacaagat cctgtattat tatatattct tttgtggtat gcacttgccg catgcccgat
   109441 gaaaggttta ttgctttaat tccatgcgca ttgaatatat tggtatcact gcctccaccc
   109501 gaaactgcca gagccggctt tatgccaata cttcgtaacg cattagatgc aattaaaaca
   109561 atgggatcat tgtccctgat ggtatagcct tcataatcca tagtgctttc aatctttact
   109621 tttgctttat atttctgtgc aattgttttt gctgttcttt caatttctct ttttaccgaa
   109681 agcactctgg tcttttgccg tgaacgggtt tcaagagtac attccgcttc ctctgcaaca
   109741 atattggttg cccgtccacc ttttactgtt ccaacattta ctgttgtttc gctgtcaagc
   109801 ctgccatggg ggatagcagc aataatttca gaaagaactc gtatagcact tatccctttt
   109861 tcaggttcca taccggcatg tgcagccttc ccctttataa taattttcat gctgatatga
   109921 taaggtgctg ccacaataat tttacctact ggtccatcgc tatcaaacac aaatgcatac
   109981 tttgctttaa ccactgacag gtcaaacccc tttgccccaa acaatccaac ttcctctgca
   110041 catgaaaata aaaactcaat atggccatgt ggtaaactat gctcttgtaa cagtgatatt
   110101 gcttctagaa acgctgcaat tgccgattta tcatcagccc ctaatattgt ccttccatcc
   110161 gatactatct tattatcttt tatgattggt tgaatatttt cacacggtgt gaccgtatcc
   110221 atatgacatg aaaaaagaac aggattgtag cttttatcac caggaatacg gatcaacaca
   110281 ttgtatgaat tattacaggg gtacagtgca cattcaacac ctaatgtatc tgctttttgt
   110341 tttatatact ctataacccc tttttcttcc catgatggag aggcaatctt tgccagatca
   110401 ataaatgttg aaaccagcct ttcctgatta actaatggct tcatggtatt cctcatgcat
   110461 attttgcagt tattttcttt attttgatgc tgtaatcatc aattaattta tcacgtgcct
   110521 catcggttat accttcacag taaacatgaa ccgatggctg caaggtatct ggtaaaatta
   110581 taatccaccc atcatcatgg acaactctta caccatcaat caactcaaca tgaccatttg
   110641 ccgtggaagc tatctccctc ataaccactg ccttagcatc aactggacat ggcagtacag
   110701 tatgatacat attggtatca ggcaattcgc gtcggatttc agataatttt ttttcttccc
   110761 gtgcaacaag ttcaagtaaa agcaaaaatg caatcattgg gtcatattca tacatgctat
   110821 gcggatacac tgattcagaa tttttagaaa atattgtaaa cacatcttca aatactggtg
   110881 ttttgggcac acgtttgagt attacattat tttcttcagc tatctttccc aggcgtgatg
   110941 acgatattac aggaatacag agttctttta ttgtcctgta ttttacaaat aataatccta
   111001 acagtgttgt tatgtcatcc cactgtaaaa tgtttccatc ttcatcaact acagttagtt
   111061 gttccccatg tggcccaact attacaccaa tttcattatt aagttttgta agatttttaa
   111121 tcccatttat agaatcccgg ctttcattac gtatttcttc agtgctgtta aattcacgga
   111181 tctgtcccct aagaacagtg gctgtacatg aaaacgcagc cagaagtgaa gggaaaatat
   111241 gcgtggtagt tccataaaaa cagtcaacta tcacattata ttgttttttg gctattatct
   111301 ctttatttac aaaatttgaa acaaacatga tatatgattc aacattatgc gttggataat
   111361 acaacctgcc aacctcaaac gcactgctac gcgggtaatc gcctctaaaa aatatatttt
   111421 caatttttct tctttccaca ttgttaagtt caaacccctg tttattaaac attcttatca
   111481 ggttaaactg taaatcagta tgtggagaaa tctgcacata aatcccaagg tcagcatttt
   111541 cataaaccac tgaatattta ttaattggta tacttgcaat ctccaggtcg tatacttcta
   111601 ctcccattga caaaaccccg gctgttaatg cacgtttaat aagtcgtgat gcccgagtgg
   111661 tatcccggct cacaatgact tttttgttct tacccaagaa agcaccaatg gccgaaccca
   111721 attttgcagc aaattcaggt gtaatcttta tattgaatga tccatagaca ccttcagcag
   111781 aaaacaaaat ctttttttca gtcttacccc atataaggtc agaattaagc tgtgtaccct
   111841 gatcaattat cttctccggc catattcgta tccccgaagc aatcttagca ttattgccta
   111901 tctggcaatc atcactgatt acagcacctt cataaattga gacagtatct tcaataacat
   111961 tgcgtttgcc aatgatggca cctcgtattt cggtctttgt tccaataatg gtattatgca
   112021 gcaccacact tcttcgcacc gaggcatttt catggattac tgtattatcg ccaatgacac
   112081 tataatcaga aacctcagcg ccttttttga ttttcacata gttgcccagt acaacaggcc
   112141 cggtcactgt tgcttcagga tgtatttcca catccttgcc tgcccatata ttttcaccaa
   112201 tttttttccc tgggaaacta atcttaacca taccattgag tatatcctga tgtactttcc
   112261 gatacgcctc cagattgcct atgtcacacc agtagccgtc agaaatatac ccataaatag
   112321 ggatattatt tttctgtagc agcgggaaaa ggtcatagga aaaatcaaat ttttcatttg
   112381 cagggacaaa ccgttccatg atttcaggtt caatgatata gagccctgta ttaacggtat
   112441 cactgaaaac ctcgccccat gatggctttt caagaaacct ttctatggtg ccatcatcgc
   112501 gcgcaataac aataccatac tctatagggt cagaaacatg tgtcagggcg atagtaaccg
   112561 gggatttatt ctttttatga aaatcaatga tatcctgcaa attgaaatca tagatcccat
   112621 caccacttaa gactataaat gtgtcatcat aatctttcag agctaacttt accccacctg
   112681 cagttcccag aggtaattcc tcaatactat aatctattga aacctcccag aatgtaccat
   112741 ctccaaaata attaaccaca ttttcaggaa gataaaataa actcattgtt atatcattaa
   112801 taccatactg tttcagtaaa tacactgagt attcaacgac aggcctgttt agtaccggta
   112861 tcattggttt tggcctgtta catgttaaag gccgaagccg cgttccttcg ccacctgcca
   112921 ttataatagc tttcatatta actccaggtt ttgaatacaa aaggtgtaca tgataatact
   112981 atcccatgca taaagtttca ggtcaagtga aatatacata ttaccaaaaa catgacaaaa
   113041 ataacgatat tctgtacaat aaaaattgta caatatcaaa agtaaaataa ttgaaaaaat
   113101 atatcactat tagatagttg gattaatgtt ttaaaagacc cttttcctaa atgggggaat
   113161 accctgagga aaagagcatt tttctgttat tataataatt attttttata ttttagcaat
   113221 tataggagtt gattatgagt aacccaatta aagaatctac actggaacgc tggatccaga
   113281 accagatgaa ggtctgggag gcagaaaaaa aggttattga aaaagaagat gtaccccgcc
   113341 cttttatcac tatttccagg caatacggct gttttgcaaa tgatattgcc caagccattg
   113401 ccacaagcct taatgattta cagcgttctg acaaatggat tacctatgac aaaaatttga
   113461 ttaacaaaat tgaggagcag tataacattt cagaaaagat tgtggaaact atcgacacta
   113521 aaaagcgtga agaaatgagc gaattatggc ggaccatgct ttctgatctg ccaccacagg
   113581 cggctgtttt ccagaaactt gccaatacca tacgttcact tgcaatccat ggaaatgcca
   113641 ttctggtggg gagaggttcg gtaattatta caaaagggtt acgctttgga ctgcatgtac
   113701 gctttgttgc tccgcttcaa tatcgcattc agaaagttat ggaaatccag aagattaaag
   113761 acagacttac agctgaaaag cttatcgaag aaaaagataa acagcgccat gaattcatga
   113821 ctcagtatat caaatttgat gcttacgacc ctgcatcata tgaccttact ttcaatattg
   113881 cccagttcac acctgaccag atagccagta tagtgattgc cgcattaaag gcacggaagc
   113941 ttatataagt attgagcatg ttaaaaaaag aggcggtttt aaaaccgcct ttttttattt
   114001 ccgtttacct ttcagttcat atacttcgcg catgatggga ttgccatcct tgtcaaaaac
   114061 aagcactgta acataattaa aaaatataaa atccttccta tatggagata catgatacga
   114121 acgtacaact aagatgtctc ttactggcaa gaaataaagt ttatttgtta aaggattaat
   114181 tcctgatttt gtattctttg gatacgcctg aaatattttt ttccctttac tgtcagtata
   114241 ttcataattg gctatatcat caggataagg aacaaaattt cgtatgcgct ccttttcagg
   114301 gatcggcatc tcaaagctgg atttagtttt ctcctctttt tcataggtag caataatatt
   114361 gacatatata tccatgggat aatccgttaa attttcaaac aacacctcaa cttcaaggac
   114421 atctcctttc cctgctatat ccactctcct gttaaaataa atatctttta ttttgacctg
   114481 attgctatat aaagactgaa caggaatatc caatttttga gtagtctgtg ccagcaatac
   114541 agatgtaaac aataccataa ttattgttaa aaaatttttc ttcatataaa ccgcccatct
   114601 tacagtgttt cttattatct atcggtcaat gtgccagcaa aatttagaag aatacaccat
   114661 ctacgcatta atacaatcaa gtagctgacg atgaatgagc ccattggtgg cacatatttc
   114721 agggtattca gggtcaaaag cctctccagt atatttagta accatccctc ccgcttcttc
   114781 cacaataagt gcaccagctg ccacatccca tggatacagc atgggctccc agtatccatc
   114841 aaaccttcca catgccacat atgaaaggtc aatggctgcc gatcccattc gccgtatacc
   114901 ctgaacatgc ggcaaaacat tattaaactg tttcagattg tttacatccg ggtcatcttt
   114961 cttatatgga aaaccggtag caataattga aatgccaata tcctgaatct gagaaacata
   115021 tatgcgtttt gtattgagat aggcaccgcc ccccttccat gcataaaaac attcatcgcg
   115081 taatggttca tagaccacac cagcaactgt tgatgctatc tccctggaat ataacgcaac
   115141 agtaacacaa aagtgcggaa tcttgtgtgc aaagttattg gtagcatcta atggatcaac
   115201 ataccataca taattgctgc tggatttttt taaacttccc tcttctgcaa caatgccatg
   115261 gtcaggaaat gtgtttttta tgctttcaac aattaatttt tcagattcat aatctatatt
   115321 ggttactaaa tttgtcctgc ttttgtaatc aactgttaca tcgttgctgt aatagccttc
   115381 cagtatgatt gctcccgcct gtttgcaaac gtggatagca tattcagtga ttgctttata
   115441 atccatacct acaactcaac acctgctcca agtttaaacg taatttcatg atggtcctct
   115501 gatgtatcag ccagttcaaa acccagtgac tggtaaagga aaatattttt atgaatatat
   115561 tcctctacac tagcagtaaa tacatgtgaa agtgacctga atcctgagaa aggatcacct
   115621 tttgtccatt tatctgtgtt aatgccaaac catccctgcc cattgccaaa ttttgcatat
   115681 ggatcatcta tatttatatt tccaaaatac attcgttctg taaggctaaa attgaacgaa
   115741 agcctcttct gaccggttag ttcattatac ctgttaagaa caactcctat actaaaatca
   115801 tatacaccag catacaaaca tgctggtaca gtatggtcaa aaaagtaaaa ttcattgata
   115861 gtaagctttg tataataacg taaatattta tcaaatgtgt atgtatatac aaaatcaaaa
   115921 taatcagcat caaaggtttt cccgcttgat ttttccccat cgcgcataat aagccgtggg
   115981 tacatggcaa tatcaaccct ggccttatcc tgagtaaaac caaataatac ccccatttta
   116041 taatcattaa ggtgtttatt ttcaccaaac acatcaccgc tacggtggtg attgccgaga
   116101 acaccaaagc ctaaatagta cccccctgca ttaaacatcc cttcaacttt ttccatccat
   116161 gcattgacat ctggcatcaa cgctccatag taaggattgc cagttgttat gcgtgcttcc
   116221 catccatcaa atggtccggc atcaatataa aacagatatg gcatattggg accaatgcca
   116281 ttatcactga caatctcaaa acctccacca tcaagaaaat agcctatccg tgacattcct
   116341 tcaaacgtgg caaaaagcac tctcttcttt tcctgtggtt tttcttcttc cacaacctct
   116401 tctttagtaa ccttcttttc ttctttttca gcttcttcca tctgagattt tgcctgtgca
   116461 attagcttct gtttataggc atcaggaagc catttgataa cctcctgatt tttgggatcc
   116521 aatttcaaaa catgctcacc tgttgcaaca gccttgtcaa ataatcctgt cttatagtat
   116581 aaataaccaa gcctctccag tgatggtatt tgtacaggat tgcgccgcac ggcctcttca
   116641 tatgcaggga tagctttatc aatttcacct ttgagttcat aaacatatcc catattatgc
   116701 aatgtcttgg ccgagcgtac attatataac ttaattgatt cgtccagttt ctgcagtgat
   116761 tcatcaaatt tttgcttcct gcccagttca accccttctc tggttaattc ttcagggtcc
   116821 tgagcataca gagccgtcac acaaatgcaa aataatacag taattatttt tttgtacata
   116881 acccttccct tactttacca atttataaca attaacagta ctatatacta ttgagaaatg
   116941 ccacagaaat gcaactaaaa attacattta agttttattc taaaaaagcc aataattata
   117001 acagtataag tattgctcta ttcgcaatgt accgctaatg tgacataata taggggggca
   117061 tcatgaaaaa gaccattatg gtaattggtg gaggcctttt acaggtacca gttattcaga
   117121 cagctcaaaa aatggggcta caggtgatag ttactgatta taaccccaat gctctgggaa
   117181 tgaagtatgc tgatatcccc attgtcatga gtacccgtga tattgaaggt tcagtccgcg
   117241 ttgcaaaaaa acagcatgag attaccccta tatctggcgt tttgactgtt ggaactgatg
   117301 cttcagccac tgtcgctgct gtagcaaatg cacttaacct ccccggtata aaatttgagg
   117361 atgccattgc tgcaaccaat aaaattaaaa tgcgcacgag gttttttgag cacggtgttc
   117421 cttcacctca ttttttccct gtatggtctc tttctgatgc aaagaaggca tgcaaggttc
   117481 tgggatttcc tgtcgttata aaaccatctg ataatatggg cgcacgcggt gtggtacgta
   117541 ttgacaataa aaaccagata gctgatgcat ttgcatttgc caaaagcgcc tcacccagtg
   117601 gcgaattgat tatagaagaa tttatggaag gccatgaatt atccatagat gctgttgtat
   117661 ttaacggtga agtaaccttc accggcattg ccgaccgcat catagaatat cctccacact
   117721 ttgttgaaac tgggcacacc atgccatcac agttgccaaa agaagtgcag gacagtgcct
   117781 gtgaagttat gaaagccggg attaaagcgt taggaattca tcttggttgt gccaaaggtg
   117841 atattaaaat aacaaaggat ggcccaaaaa ttggggaact ggctgccagg ctttccggtg
   117901 gttttatgtc tgcgtatacc tacccgcttt caaccggcgt taatttaatg cgtgctgcca
   117961 ttgaggtagc attgggacag gaaccaggca accttgaacc actgtataac agggtatcga
   118021 ttgaacgcgc aatcatcaca aagcctggta tagtaaaaaa gatatgtggt gtagaagaag
   118081 cattgaagat ccccggcatt gcagaaatat tcattaccgt aaaacctggt gataaagtaa
   118141 aaaaaccaac atccaatgtt gaaaaagcag gacatatcat tgctgttgct gatacactga
   118201 aagaagcaga agaagctgta cgaaagtgca aagaaatgat acacatagaa atatgtgatg
   118261 aatcagaact ttccatggat accatcaaca tttctgcacg ggaaaggttg cgcaaagtat
   118321 gctacgtatg caagcagtgc aatggcaagg attgcgccag cggcgttccc ggtatgggcg
   118381 gtgcaggcag tggagcttca ttcaagcgaa acagtgaagc actccttaat tataaaatca
   118441 atactcgtgt cattcatgat gtaaccaatc ccgatacatc tacttcactt tttagcatca
   118501 acctgtcctt ccccgttatg gcagcaccca ttaccggagc tattaccaat ttaggtgggg
   118561 cgatagatga attagattac aatatagctg ttgtaaaagg atgcatggat gcaggcacca
   118621 ttgcattctt aggtgacggc gcagatccag aaaaatataa aatagctatg gaagcgttat
   118681 ctcaatttgg cggtatgggc atacccatat tcaagccacg agcaaataac gaagaaatta
   118741 ttaaacgcat taaagctgca gaaacagcag gcgctattgc tgttggcatg gatattgatg
   118801 ccattgtatt caagaccatg gaattaaaac atcaggcagt aaagccaaaa agcttaaccg
   118861 agcttaaaag cataatatca tccacacatc tgccatttat actcaaaggc attatgaatc
   118921 cacgcgatgc tgctatggct gttgaggctg gcgcacatgc aattgttgta tccaaccatg
   118981 gcggcagagt acttgatgaa atggtgggca ccatggatgt acttgaagac attgtgcgcg
   119041 aggtacatgg acacattcat atattaattg atgggggttt tagaacaggg gttgacattt
   119101 taaaagcatt agcattaggt gctgaatatg tgcttattgg caggcctgtt gccattgctg
   119161 ctgtgggaat gggttctgag gctgttgcct attaccttaa atcattacag gcagagctta
   119221 aaaaagccat gatactgaca ggctgcgcaa caatacatga tatcacaggt gatatcgttc
   119281 gcaaaataca tcataattca taccagccac tagaggtacg atagtacatt gtatgggaaa
   119341 tgctctatta ttactactgc tcaatgcagc ggtgccaata catttcatta tttttaattg
   119401 aaaaatattt tttaaaaatt ataacactat tccaaatttt cagaaagtgg actattatgt
   119461 attcccgcat attaaagaca ttgctttaaa aaattccttg attattagtc agattattct
   119521 tactgtgtta aacattacaa cgaagggaag taaatgatca aattatccag atggtatatt
   119581 gcaatagcta ttgtaacagt aactatcgcc acgcatgcac ttcagtttac tgacattcat
   119641 ggtatatggc aattactcta ccgtggcaac tatggatatg aattcagatt ctataaaaat
   119701 taccaggctg tgtgcattat ctacatgcag accaatgccg ttgtcttcaa aggtgtatat
   119761 acctttgaca atccacaaac cattcgcatt aacattaacc agatgaaaaa tgccgatagt
   119821 tgctgtaatg tcaataccac tactggcttt gctcaggtaa aatcttcata cttccttttt
   119881 aatgctacat tgataaccaa aaacaacaaa aaaatgcttg aattaaagcc gctgtccatt
   119941 gtcattgatg gaattaattc tgaaggatat tttgaaccag tcattctatt gcagcgcatg
   120001 taaccaccat gagctatcga acaattacta ttccgtataa agaccccata gatgcggttg
   120061 tgtatccctg ttcccaaagt gtgggtacca ttgtgtacag ccatgggctc ttttcttcca
   120121 aagatgccta caagatacat cagatagcac cagcccttca tgatagcggc tatacggtga
   120181 taagtttcag ctacggatac actcgtcact atacaaccgg aaacgctctt acgattgcgc
   120241 tgccacagtg tgcagatgaa ctcaatgcag tgctggaata cgccgccaat acttacaacc
   120301 ctagccttca tattattggt tcaagtatgg gtggtgctat agcgctgtac tatgcagcaa
   120361 ccgtgccata cattttttct ttaaaaagct taagcattat agcaacacct gtgcatataa
   120421 atcatttagt cagggcgata gctccgcaag gcatctcagg taattatgta accgttggcg
   120481 aatacacaat tgatgcacaa tcgttgcaac agatgaatac cattaacctg ataaaactta
   120541 tcccacgcgt tgacatcccc gtgtgtatta ttcacgggca gaatgacgat gtggtgccag
   120601 tagctcatgc ccatgatatt cagaagaatc tatgcgtacc ccataccatg cacatcatcc
   120661 ccgacggcga ccatacatta acagcacaaa ggcatgttga attaatcttg caatacatag
   120721 tgtcatggct taaactgcat ccgtagtacc ttttgctatt aatacaaatt gaataaacct
   120781 tgacataata ttgttaacat attatatttg tattgttaga agttaaaata tatggagcat
   120841 gtatatataa aaggttaaaa taatgcttac caatctaaat caatgtccaa atcctataga
   120901 atatcaaaac caaaatataa atgaggatat atttaattat ttaacggaaa agttaaaaga
   120961 aaaaactatt cttcattata aaaaagcaat tcttttacaa ggagatgttt taaataaaga
   121021 ctattttgat aatgacattt ttgatttaat agttacatcg cctccttata atgttgatat
   121081 caaatataat acacataaag acaacatttc ttatgaagaa tatcttcaat tttcagaagc
   121141 atggatgagc aattgttata aatggagcaa gaaacaagca cgcttatgtt taaacattcc
   121201 actggataaa aataaaggcg ggcagcgcag cgtaggtgca gatcttacaa tacttgccca
   121261 aaaagtaggg tggaaatacc actcaacaat tatctggaat gaaggtaata tctcacgtag
   121321 aacggcttgg ggatcatggt tatcagcagc ggccccatat gttattgcac ctgttgaact
   121381 tattgtaata ctttataaag aagaatggaa aaaaactcaa ggttcaaaaa tttcagatat
   121441 atcaaaagat gaatttatgc aatggacaaa cgggttatgg acattcaatg gagaaagtaa
   121501 aaaaagaatt ggtcatccag caccattccc attagaatta ccatatagat gtataaaatt
   121561 atttagtttt gttcacgata tagtttttga tccatttgct ggtagtggaa ctaccctcat
   121621 agcagcaaat aatattaata gatattcaat tggattagaa attgattcct catattgtga
   121681 gctctcaaaa caaaggatat tacaagaaaa tggaatgcta ttttaattat gtaaatatat
   121741 tatgcaaaaa caaaaaacta ttactgattt aatcattgaa ttttttaaaa accatcctaa
   121801 agaagatatg ccccatggac cagtagttga ttgggttgaa gaacaatata ccaaattata
   121861 taatagaaaa ccacgagaca tatggcgttc aatacgtcgt ttgcaccaag aaggtttttt
   121921 aataaaagtc aaaaaaggta tttatagata tgatcccgat atagcaacca ataagcatct
   121981 tgaagaattc tctcaagaat taaaaaatca aattttagaa cgtgataact ataaatgtgt
   122041 aatatgtgga cgtactgcta aagaaggata tgaattacat gtagatcaca ttttaccaaa
   122101 agataaggga ggtaaagcta cattagacaa cggacaaaca ctttgttcaa tttgtaattt
   122161 cagaaagaaa aattataatc aaacagaaag cggtaaaaga atgttcattc gtttgttaaa
   122221 aattgcaaaa gaagttggag acattaaaac acaaaaattt tgcgaagaaa ttttaaacac
   122281 ttatgaaaaa ttcaacataa atggacatat aaaatgggat aaataaaaag tttctaatat
   122341 ccaata
//
LOCUS       DAORYL010000003       113932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_117_length_113932_cov_13.350633, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000003 DAORYL010000000
VERSION     DAORYL010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 113932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 113932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..113932
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117_length_113932_cov_13.350633"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..501
                     /locus_tag="PLV81_01180"
     CDS             1..501
                     /locus_tag="PLV81_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005993277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOM08746.1"
                     /translation="MKEIQDGRKDKLSMVVFSGDLDKLLAAFIIATGAVAMGMEVVMF
                     FTFWATPVLRDKKKKGKGKNLFGKMFSFMLPKGASKVKLSKMNMGGMGTGMMKYLMKK
                     KNIQDLDGMIALAAELGVKIYVCEMSMDLMGFKMDELIDYPDMKSVGVATFLQEASDS
                     KIQLFI"
     gene            629..859
                     /locus_tag="PLV81_01185"
     CDS             629..859
                     /locus_tag="PLV81_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOM08747.1"
                     /translation="MKADVTMDLKGLACPLPVVKVSQQIKKMQVGQILMAETTDPGAL
                     ADFPAWAKTSGNEIIDTVKDGNVTKFFIKKLK"
     gene            883..1605
                     /locus_tag="PLV81_01190"
     CDS             883..1605
                     /locus_tag="PLV81_01190"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HOM08748.1"
                     /translation="MLGYISYFILVPMVYIAFVTLIVGLMYKFYVIFRAPVPAGTGAI
                     FPKKGSKVLGLIYDAMIVPNVYNKQKVFWFIIMIFHIAFFLLFLGHLELVDDFAFIQI
                     IPHKIFLGGGVVGITLIITTLYFLFRRFGTPYREISIPEDFVILLVLFFCILFGSILH
                     LAERYSDWGAVLRVNVDVYREWLQSMILLKPELSYKITELSHYTILVLHVLFANIFLM
                     MFPFSKMVHSVFTFLAHYRKRK"
     gene            1608..2855
                     /locus_tag="PLV81_01195"
     CDS             1608..2855
                     /locus_tag="PLV81_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOM08749.1"
                     /translation="MDKEKVLELKQVIEKKLNRPMLYYLDLCTRCGLCYDTCHAYQGI
                     PKKEYSPVGRAEVVRKVFRKYFRPTGKLFPWWGDAQEFDENLMEELREAAFSCTGCRR
                     CTMFCPFGIDMPYILGIAKELLIAYGNAPEELVMLADGAVEKGKSVDMFKEGFKGVIK
                     DLEKEVQKLINAPTSDGLIPMEKKGANILFVGLSGAHSIVYPAAIFNAAKEDWTLSYF
                     EAVNFGFFAGDSEKANFIANRVINEAKELGVKEIVIVECGTAYRIPKFLMGPLPFKVS
                     SIVEVIHRYIKEGRIKIKEGAITEPVTWHDPCQLGRNGGVYDQPREVLNMLAKDYREM
                     NPTREYNWCCGGGGGLVALDNEEFRIKSGKPKKEQIEKTGAKMVVTACENCHLQLSTI
                     SAGYGMGVTIESFTKLVGENLIV"
     gene            complement(2892..5414)
                     /locus_tag="PLV81_01200"
     CDS             complement(2892..5414)
                     /locus_tag="PLV81_01200"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOM08750.1"
                     /translation="MHDIIASLNIFMLPPLITLLTSIVIAGIAILKGKLKKENILFAL
                     FCLWYALLTPNFLGHFIIKDVNTILKMERSIHTLYVFIPFIGMLFYHTALNIKRTYLE
                     IITFIVSAIFALLVHTPYYITDLLTYPWGHIAKGGIVFQIFGVYAFSVLIYGIVISIL
                     KLKQEKNELVRLKIKYIILALNLIGIFTACNVPAMLGYNFYPLSNLMFIPLIFLGYGI
                     LRYRLMDIQSVLHVTFIWLCISSVIVVPNVIIFNILYPFFKSIDKSLLFFLLTIWFAV
                     NYLYFIKVQPLIDQVFNRRKYNLKKVESQFINDIALLKDLHLLLNELSYILKRTLLIK
                     NVEIFIKAEDKENELYNLNGDVIVIDEQVQDYLLKNPWVIEKKLIENKPQYEHVKNIL
                     LPWFEKLSAEYIIPLEHNEFIGIIALSERANLKALSNDEIEFLQNVKSASSIAIANSL
                     MYHSLSNLKNNLEALVMQRTEQLQKKNEQMEFELKLAKTVQKTLLPSILPANNQLYAS
                     VYFRPYMEVSGDFFLMEQLDDTHYMIGIVDVSGHGIPSALLTSMIKTEIENLSRLYRS
                     TSAICTQLNKKLTPILQESGFYFTLFLGIIDFVNMELLYTNCGHTDIYIITLNDTVYP
                     LTTDSLFIGADEHINYPECLFELNTKDRIILYSDGITETRAKSGHLYGNERFTNILVA
                     SREFAPQDQIDIIIKDLENFIQSEDGVIRDDVTLCVIEIGEPVAVENYLKKAITLYKK
                     KNYNEALSILNSISTVPLSASYNYLKAKLLMKNKHYEEAKKAILQALKEKPDHKEFNY
                     LFGQICFALKDYQTALDTFADLSMMQPYKKSNEFVEIIKKKI"
     gene            complement(5522..6202)
                     /locus_tag="PLV81_01205"
     CDS             complement(5522..6202)
                     /locus_tag="PLV81_01205"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOM08751.1"
                     /translation="MIYIAFDIDGTLYDCSPVVGMAFEQGIDTFLVQHPELNLRKPST
                     DEIMKLVGIPVDEIFALLFPALSQELSHTLNDYCTVKLAELVLQQKGTLLPGVVETVK
                     VLYNRGYGLVTASNGRKEYVEAVIDAYKLRQYFFTITALEDKHLHDKTQLLAYYKESL
                     PHCDIVIMAGDRANDMVAAHKNNVPFIACAFGHNDEEIAHCRWIAHSFYEIPDIVDSI
                     IKEMKPVD"
     gene            6330..7250
                     /locus_tag="PLV81_01210"
     CDS             6330..7250
                     /locus_tag="PLV81_01210"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOM08752.1"
                     /translation="MNLSVTIGNLQLKNPITVASGTAGYGEELSQFVDIGSLGAIFTK
                     GLSLKPRAGNPGPRIAETPSGMLNSIGLENVGLEAFLSHKLPYLQKANATVIPNIAGH
                     SIEENVELAKTLSNTNGIAAIELNVSCPNVKEGGIAFGQDVAIFSRLLEKVRSVTRGV
                     LIVKLSPNVTDITQFAKAAKEIGADAVSAVNTFVGMKIDIATGKPYFANTFAGLSGPA
                     IKPIALRMVYQIVQNVDIPVIGIGGIFSFEDVLEFLMAGAQAIQIGTGNLIHPDISAV
                     ILQQLENYMKMHSIDTVKDLIGIAQKRSNQ"
     gene            7247..8299
                     /gene="hisC"
                     /locus_tag="PLV81_01215"
     CDS             7247..8299
                     /gene="hisC"
                     /locus_tag="PLV81_01215"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOM08753.1"
                     /translation="MKYWNKRLQKMTEYIPGEQPDNLNEFIKLNTNENPFPPSSAVLD
                     ALQKACNGDLRRYPNPNAQMVRELFAKKNSLAADNVFVANGSDEIFTLLFRGFIEPDG
                     IAAFPYPSYALYYTMAEANGIAYDKITLDDNFDIPFDEFLKKKYNLVIIANPNNPTGK
                     GVPIDQIKKFCSKFKGLLVVDEAYVDFYNETALPLVKEFDNIVITRSFSKSYSLAGLR
                     VGLAIAHKEIIHGFIKLKDSYNVDRIAQAGAYVALLDDKGFKYNISMVRNNKEYLEES
                     LEALGFINVPSRANFVFTKHPDIDARTLYEKLKEQKILVRYFAGPIQSEYIRISVGTM
                     MEIKKLIKVLSEIISG"
     gene            complement(8268..8906)
                     /locus_tag="PLV81_01220"
     CDS             complement(8268..8906)
                     /locus_tag="PLV81_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08754.1"
                     /translation="MQRLSILVVLSLFIVSACATANEKNVQQTVGDSSTQQSAVSNDY
                     AVPEFQYTTQEGIKLNAMYNTKLNDLGLEIKKIEPLAPRIRTFGFYYDNKTKEKNKLY
                     LGFDCDVGGTITGADSYPLRAWNIIFKNLNPVLTKSKILHQILQDENIQGLVISFYWF
                     FEGSEGIILWIEKKDIEQYMQNKIIYPELIARSTTTRLDGKIINLILFQKAP"
     gene            complement(8933..9742)
                     /locus_tag="PLV81_01225"
     CDS             complement(8933..9742)
                     /locus_tag="PLV81_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08755.1"
                     /translation="MNSLRITGIIRHYTRRARDQIYNAGLTIFKAANCEQHLDDIGAC
                     VDELIKNAVKANYKHLIIVEALQRQFQLQYKDKTGKQVQEYIKDILKDKIQFDTLASI
                     VLKKENIAQKVREVLNQEGLFLLIKNKAFSENRSLTSDDLQSIEKLKLFVELQQKLKQ
                     YDIRIILKIELRDNYIYIEITNTAPILSHDLQRIYAKREEYRRYKDEGREYEFFINNI
                     DTSDSGFGLGYAKIDSYLYNLGIDPEKAVTVISINNTTAMLSIPVEALKKL"
     gene            complement(9748..>11214)
                     /locus_tag="PLV81_01230"
     CDS             complement(9748..>11214)
                     /locus_tag="PLV81_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08756.1"
                     /translation="ELLSEKYDSFKKLSNFVQAIKEQPKTAEEAPRPSRIPQNLSQLY
                     KNSIYQIFEFGLIEKENQKNLLKLLSEFKKMKDPLSSEIDARKIRRQIARFYYDIYAK
                     VFVRSQSESAVPQPVKLMLRFGFFDETMIEQEQLAELHEISMRRTEQTDIPILYEDEF
                     LTKIFNGEENPSINEMGLSYEAHLREEQKHIKGSNAKDESDNVQKVIYEINHRIATAS
                     SVCSGSTATSFPILTSMNMRGSISHYFVSKKKLESIVNALRDVDYSVFYRETAVMIGN
                     TKELIKEEVVPYFILLPSFGTKTMLWQELDGTNRRTRGRIVVPILFMGDMMPTMAHTF
                     ATFRWELNRTIMGGMWADPIEGGLTGAYFDYINTFKKNPKLSLEAKQKVVERFKSIRN
                     NRDRFADDYIQWVLYEKDGIMKLNNVVRDMFYRHIPFKKELRDRLENMPAYTEIANRF
                     RNVFNREVGNYERKFKKYQRDDGTLPEALQKFMEFLYK"
     assembly_gap    11215..11314
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11315..11769)
                     /locus_tag="PLV81_01235"
     CDS             complement(<11315..11769)
                     /locus_tag="PLV81_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOM08757.1"
                     /translation="MENNGTEKIRKGEILILQGEEPKFIYYLQSGSVEILSAPSEYEG
                     LDSKIIVEKSKRVGIINEKSVIAGLSLLFTEPYKKTIRAIEDCVVKKYPLREGGFRQI
                     VVDDPSFAALLLTHVLKRLELSLNDVKKYTLLYQNLCRVNDNFSLVAGEV"
     gene            complement(11926..12273)
                     /locus_tag="PLV81_01240"
     CDS             complement(11926..12273)
                     /locus_tag="PLV81_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOM08758.1"
                     /translation="MGDRSKKFKPKELHFVDSGFSDLETHVKREKQKARELRHSSWWK
                     KRVAPGICHYCGRKFKPSELTMDHVIPLSRGGYSEKINIVPCCKECNIKKKSLLPVEW
                     EEYIQNLKKIGMK"
     gene            complement(12279..13211)
                     /gene="fmt"
                     /locus_tag="PLV81_01245"
     CDS             complement(12279..13211)
                     /gene="fmt"
                     /locus_tag="PLV81_01245"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOM08759.1"
                     /translation="MKIGFFGTPEIASYCLEHLIKHYEIAFVVTSPDKPKGRGKHLLP
                     TPVKEKALEYGIVCFQPTTLKDEQLAETLRQFNCDIFVVVAYGKLIPRSIFSIPPLQT
                     INLHPSLLPKYRGAAPVEWALYNGEQQTGVTVQRINEELDAGDIVLQSTVTIAPNETA
                     QDVYEKILPLGADMLVRAIAGLHDGTITPVPQDHSQATYCGKITSETARINWDIPSIH
                     IHNMVRAFNPKPVAWTTFRGKIIKIWKTLLSDSSCNNSTARPGTVVVQAKRLFVCTFD
                     GTIEVMSLQPETKKPMDASAFINGYRLQHGDAFE"
     gene            complement(13211..13729)
                     /gene="def"
                     /locus_tag="PLV81_01250"
     CDS             complement(13211..13729)
                     /gene="def"
                     /locus_tag="PLV81_01250"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOM08760.1"
                     /translation="MVTDTQHRLVYYGNKTLRSVAQEITQITQEIRNLTDEMFAIMHK
                     EQGIGLAAPQIDVPLQLLVIDIRSNKGPMMALINPKITAFSDETDGYEEGCLSLPGIT
                     ADIIRPVSIQVEGIAINEKPVKFEASGLLARVLQHEIDHLHGKLFIDYLEDYKRKELN
                     AQLKKIKKLNRA"
     gene            14009..15505
                     /locus_tag="PLV81_01255"
     CDS             14009..15505
                     /locus_tag="PLV81_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004491667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOM08761.1"
                     /translation="MPFKEYDNYDATGLAELIKKKKVSIKEVIIAAIERIEKYNPTLN
                     AVITPMYDNIDRIIKELPKGYFYGVPFLIKDLLTAYKGVPMASGCRAYKNYIPDFDSE
                     VAKRYKNAGLVILGKTNTPEFGLVAYTEPEIFGPTRNPWNTALTPGGSSGGSAAAVAS
                     GMVPFAGGGDGGGSIRIPSAYCGLFGLKPSRGRVPTGPEYGEIWQGAAVEHVLTRSVR
                     DSAAMLDILQGSDPGAPYIIEPPKMPYSKEIQKAPGKLKIALSTDSPIGSPVHPECKN
                     AALEAAKLCEKLGHYVDIDKPNIDGLALAKSYLMLYFGEVAAEIRTIGIKLGRKPKRS
                     DVEVTTWTLGLLGRTYTAGDFVMALREWNKAARAMGQFFTKYDIYLTPTTAQPPAPIG
                     SLAPKPIELFGSRIVNIFGLGKLAIASGVVDKLAVQNLAQVPFTQLANVTGIPAMSVP
                     LHWGPDGLPYGVQFMAAFGQEALLLRLAAQLEKAKPWFNKRQELYHRG"
     gene            15513..16253
                     /locus_tag="PLV81_01260"
     CDS             15513..16253
                     /locus_tag="PLV81_01260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOM08762.1"
                     /translation="MKKVLNALIEILFFFYRPIFYLLVIIDTTILGILTITLSFFDPT
                     GNTIHYIGVFWSRLNLFLSGVRVRVHNREYIKKDQPYIVMMNHQSHYDVWAAIGYIPL
                     QLRWVMKMELRKIPIFGLGCERMGHIYIDRGDSHQARESLKAAGEKIRNGASVVFFPE
                     GTRSPDGKLLPFKKGGFVIAIESRVPILPITGLGSRAILPKGSMAVRPGTIDLIIHPP
                     IEVTGYTQENKEELMEKVRAVIQSKLVL"
     gene            16416..18791
                     /locus_tag="PLV81_01265"
     CDS             16416..18791
                     /locus_tag="PLV81_01265"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOM08763.1"
                     /translation="MDGAMGTMIFNALPGYKGNLEILSIERPDVIASIHKQYIDAGAD
                     IIETNTFGGNALKLHEAGLDSRCEEINKAAAMLAKDVARGQAFVAGSVGPTGTLVEPM
                     GITSAETVYNAFKRQISGLVAGGVDCIAIETMTDLQEARLALLAAKDVTDIPVICSMT
                     FEQNSKTVTGTDIVTALATLSQMGADIVGANCSMGPDGLVALFKGTIDQLNELGVPLS
                     VWANAGLPELIDGKAVYSLSPKRFASLSLEFAQMGFTIIGGCCGTTPEHIRMLATAVK
                     HILINTKKTKTFHYITSRYTHINVVNHTGLLIIGERLNPTARKQFAAELKENTFTFLR
                     NESKKQQDEGAHCLDINVGVPGIDEVAMMKKAVNVLSNTVTIPLMIDSDNPFVTQAAL
                     WQYPGIPIINSISAKKQSFETMLPLLKKFGSFVVAMCLDESGIHKEASKRIAIGETLI
                     DLLQHQGIALSRIFIDPLILAESAESGAAQETLKVIEHFAKKGIKTSIGLSNISFGLP
                     QRKFINNVFLKLALQRGLYAAIVNPGAIDTTTAKPYSTEELLALDFLEGRDKNASHYI
                     AHFGTGQQQMQQKEKAISILDRIFELVVAGNTDDIEPAVKEALSQHTPETIMNDALIA
                     ALEKVGQLYSNGEYFLPQMIASADTMKKGFNLLKPLMLQKKESVLGKIVICTVKGDIH
                     DIGKNIVAMMLENHGFDVIDLGKDVPNEKVIEAIQQHKPDIVCLSSLLTTTMPNMGTI
                     AHEIKNQKLLCKLMIGGAVVTKEYADSIGAFYGKDAVEAVAVAKGIVKKSQ"
     gene            18938..19456
                     /locus_tag="PLV81_01270"
     CDS             18938..19456
                     /locus_tag="PLV81_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOM08764.1"
                     /translation="MKGTRSFLLFFLPFVIFAYISFIVFQQACDYAMNVQAKKLLPFN
                     HRTHTTTYGASDCEICHSYDSNGRFNGIPTVAVCKQCHDPNKANTQVFFAGFGDNDKP
                     WESFAQQPDLVYFSHIAVMKNEKKAQCISCHGDKGNSTTTAKIKGKMAMGTCMDCHTA
                     LKISNACTVCHD"
     gene            19502..21469
                     /locus_tag="PLV81_01275"
     CDS             19502..21469
                     /locus_tag="PLV81_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOM08765.1"
                     /translation="MKLERKDFLTMIGGAVVGTGVGTLISPGPWMGLQWLVEWTQDQY
                     RPPKGEEKYLASICDQCPNKCTVQVRMIGDRAVKIETSNQGCAAGQLLLQVLYHPERV
                     KTPLKRTGKKGKIDFEPVSWENAIADISKKINDAINNKKQDQIVAINPAFPDATAMLN
                     ERIALFAGSPHVYQESGYWALSSACSLATQKTEGAIDYDFENADCVVSFGARLFEGWG
                     NPGRMHNVLNIWKQKGVKFIQVDSIATRTASLADQWVAIKPGSEPIFAMGVAHYLLKY
                     GRRSGATNFGRFTELLINKFTPEKVSELTGASLETIESVAKALLQARRPVAVAGKGGK
                     VVSSSVAEFAIIQTINSMIGSLGNAVKLVTFATPAKPVIAKKSKGLDEYIASDMTCDI
                     VLINNANPVYSSVYGERLSEKLKKASTVVLFTSLLNDSAQYADYIFPTLTSLEIPFTG
                     QAVSAKFEAKHAGDSLLAIAKNIPNIAAQFPWNSYLDVVKAVNFQAKNVANLDIPVDI
                     ITAKLEELEKMLQASGSYSISMVPFEIPVVGEGNSLALPYVLKALPNTILKGDTSWVW
                     MNPVTASKEGISEGSKIDIESSRGELDTVRVHLTKRVAYDTVAMPIGFGHEELTEYGS
                     EKGVNPKPIMSDALDPLSGYADWWLTRVKLS"
     gene            21483..22313
                     /locus_tag="PLV81_01280"
     CDS             21483..22313
                     /locus_tag="PLV81_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOM08766.1"
                     /translation="MAKMTRRWGMVIDLDKCTGCGACNIACAQENNLPIFKDDSDVPK
                     RVTFLDLIEVTNDKDPKAQYPDVKVAYIPKMCQQCSGNDPNNPHPPCVSVCPVVATDV
                     GEDGVVSQIWSRCIGCRYCQVACPYEARVFHWWKPRYEGSFKESLNPDVSIASRGTMV
                     KCTFCSHIWKRERDKAIAQGITDINAVTYTPACASACPTNAILFGDLNDPSSPVYEPK
                     IQKDPRAVRLVHSIDELRTKDPSEYEKRTKIKEYPNPKVYYLTSQQWLRDKISGFKNS
                     "
     gene            22338..23570
                     /locus_tag="PLV81_01285"
     CDS             22338..23570
                     /locus_tag="PLV81_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08767.1"
                     /translation="MSKVIDFLKNEWNSYPRNIQISLYVLFALVALFVIFAIQILFWG
                     LGYTDMNNRFAMGIWIIGDLGFVALGGGAFFTGFMLYIFRVDKLQPLINSTVLIGFMC
                     YLFTFILLVFDIGQPLRAWFGYAYPNWGPHTMPQSMLTEVVWCLTFYFIVLCIELFPI
                     ILSNKYLDRYPAVHYFAHYLHKLMWIFAAIGTFLSFFHQGSLGGGMWGVLYAKPYWFR
                     HHYFYLAIVAAIAGGPCLMMLAPKIASWVAKKELVPKDAYMTLAQIAGVTFIAYFLFR
                     VYDLYTLAKHFAPLFDRNLVDMMGGYYGWWVVGLELLASLVAIVILNVKKFRENEQYF
                     IAGLISGVLAIVMLKVDVLLHGFSIPNFPWQDFFGYNPTIQEWFIFLGSLACMTLIYM
                     WFVKYIPLFPEKHHEEAH"
     gene            complement(23650..24546)
                     /gene="folD"
                     /locus_tag="PLV81_01290"
     CDS             complement(23650..24546)
                     /gene="folD"
                     /locus_tag="PLV81_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOM08768.1"
                     /translation="MAIIIDGKAIANEIKEEVKQEVEKLEQKYGKKPALAVILVGNNP
                     ASEVYVRNKKKNSEEVGINSWQILLDEHTTQQELLTLISQFNNDNNVNGILVQLPLPS
                     HINENEIINAIDPVKDVDGFHPVNTGKLVIGEDCFIPCTPYGCQELIVRTMPDIKGKH
                     IVIVGRSNIVGKPLANLMVQKNNRANCIVTVCHTATKDIGYYTRQADILVVAVGKANM
                     ITKDMVKKGAVVIDVGINRINDPENPGKTKLVGDVKFDEVSEVAHAITPVPGGVGPMT
                     IAMLLKNTVKAFKLQNNIAINE"
     gene            complement(24571..25020)
                     /locus_tag="PLV81_01295"
     CDS             complement(24571..25020)
                     /locus_tag="PLV81_01295"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOM08769.1"
                     /translation="MSGDSNEYFEFVEDITLADIAVHCKAKTVEKIFKLNAEALLHVC
                     LEEPSKLKKVEKCHVLLQHNYLDLLLVKFLNELLFIKDTRGLLVYPETIQIIYSNDAV
                     SCEARCTGDYITNGYDINIDPKAVTMHKVKCEKINDNEWESFIVIDV"
     gene            complement(25017..>25961)
                     /locus_tag="PLV81_01300"
     CDS             complement(25017..>25961)
                     /locus_tag="PLV81_01300"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOM08770.1"
                     /translation="EENGCIANAKPEAVSGKARERGKEQVGSLGSGNHYLEFQIIRDE
                     DILNRDIASRFGITGNNQIACMIHCGSRGFGHQIATDYLDQFISVMKQKYHIDIPDRE
                     LACAPFHSKEGQAYFHAMNCAINIAFLNRQLIFHIVRDVLCTIFKKSPEELGIKLIYD
                     VCHNTAKIEQYDINGKNKKLLVHRKGATRAFTKGMQGIPELYREIGQPVLIGGSMQSA
                     SYILVGGPMAKEAFYSTVHGSGRVMSRQQAKKRFKGRDLANQMEKSGITVRTRSFSGL
                     AEEAGAAYKDIDEVIDAITRAGLSIPVARMIPIGSIKG"
     assembly_gap    25962..26061
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(26137..26559)
                     /locus_tag="PLV81_01305"
     CDS             complement(26137..26559)
                     /locus_tag="PLV81_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOM08771.1"
                     /translation="MAKKVIQVNKNPVYEIGMIATVFGKDTEFYGDLTFERSLQINGY
                     FEGEIASGDFLVIGEKATVKANIKANTVIIKGTVYGNIEAKDRIEIQSDGKLFGNIRT
                     SKLIIADGVVFEGKCEMIKSPIPVQKQKQNQKEPVAKE"
     gene            complement(26674..26895)
                     /locus_tag="PLV81_01310"
     CDS             complement(26674..26895)
                     /locus_tag="PLV81_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM08772.1"
                     /translation="MITKEKEEVLRLYNSGLDAYKKRRWDEAIDFFEKALKVDPNDGP
                     SKLYLERCKQYKLTPPPDDWDGVFTMTTK"
     gene            complement(26954..27934)
                     /locus_tag="PLV81_01315"
     CDS             complement(26954..27934)
                     /locus_tag="PLV81_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOM08773.1"
                     /translation="MTPKKDLQSILRPIEHYLKAVDEAIKNKLYTGIDLLDESSMHTF
                     KKSGKKIRASMIILSSGLNNSIPDDIIDIACAAEIIHAASLVHDDIIDNADLRRGLPT
                     VARLFGPKVAVLAGDYMYTKALEIAVGNNRTDLFPVMVDATIEMVKGELYQIQYSNID
                     NITEEHYYRIIEMKTARFMAACAKLGACVAKMDNEQSDILYNCGMQLGYAFQITDDAM
                     DYLNNAEITGKDAGNDFINGKITLPLLRLLQIVGEKEKDLLINYAKHPDNENWNYVLE
                     KATEYNVVEYCINIAAEYVQRAVKLLSVFPDTQFKNVMTELALFFIDRRY"
     gene            complement(27952..28278)
                     /locus_tag="PLV81_01320"
     CDS             complement(27952..28278)
                     /locus_tag="PLV81_01320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOM08774.1"
                     /translation="MKIKKDENLAIITLDGRIDQEMSEDLENALQKLIDEGYSNICMD
                     MTDVKHICSSALGVIVAFKRKIKNEGDIKLVITNENLLKLFQTTMLDKVFEIFESQRE
                     CLSAFD"
     gene            complement(28444..29166)
                     /locus_tag="PLV81_01325"
     CDS             complement(28444..29166)
                     /locus_tag="PLV81_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOM08775.1"
                     /translation="MANSKFITLLHYILLDVIFPSFCVYCGKLISNGDGVCNNCKSII
                     NLNIKNYSGEYVFSLEEEIHGKRKWFLDDGVWLFKYEEPVTQLVYAMKFKHNKSIARL
                     FAQYCTEAIDIMKWHFDFITYIPVSKDRLWKRGFNQSEVMARCIGKRYNIDVLNLLNI
                     KNEKVAQKKLSGNERFVNTYQKYEIIQDTSNIADKSVLIVDDVYTTGATINECARLLK
                     INGVKHVYACIVAYAMLKELIV"
     gene            complement(29156..29845)
                     /gene="queC"
                     /locus_tag="PLV81_01330"
     CDS             complement(29156..29845)
                     /gene="queC"
                     /locus_tag="PLV81_01330"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOM08776.1"
                     /translation="MAAGKCVVLLSGGLDSATAAAVAKSRELSLFALTFVYGQRHDIE
                     VDFARKIANFLHVASHEVINLPDSLFKSSSLIRTSGKEILNYHTDSIPTTYVPARNIV
                     FLSMALAYAETINATHIFIGVNAVDYSGYPDCRPEFIKAFQKVVQMGTKSGIKGNPII
                     IETPLINLTKGEIIKLGISLGMDYSLTSSCYNPQIDGTPCGVCDSCRIRQKGFSEAGI
                     PDPALHRNYGK"
     gene            complement(29860..30102)
                     /locus_tag="PLV81_01335"
     CDS             complement(29860..30102)
                     /locus_tag="PLV81_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08777.1"
                     /translation="MGLITDKIRENAEKCKSCAFLKIRNAEIDKNSVLYDEAMMTLEG
                     VYRASCTMCPFEKDFEKVYGMKPYEYFPMRIAKKEA"
     gene            complement(30285..30740)
                     /locus_tag="PLV81_01340"
     CDS             complement(30285..30740)
                     /locus_tag="PLV81_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08778.1"
                     /translation="MLIALFILLQISFSLHIYSLVLYVLRRENKYLKGFINTTISNVL
                     LAGAITTLAIIHPVYVAKVDFKLLLWLMTGFIMLIMLFIKISIARAIYKRSKDPQHFH
                     YNYFGKKVLHGTVVKFEEILIFFFTMPFFLFCGAYFIARLINLLLYKQL"
     gene            complement(30854..32170)
                     /gene="rodA"
                     /locus_tag="PLV81_01345"
     CDS             complement(30854..32170)
                     /gene="rodA"
                     /locus_tag="PLV81_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOM08779.1"
                     /translation="MFKTIKSAFQEYDVNFLFAIIALVLIGIITIYATGFDPIEKVNS
                     GLYKKQLLWFIIGAGCMFAVSRIHYRLLGDYSLYIYLILLFFLLITTIFGAKIRNTRA
                     WLSFGFFTIQPSEFMKLASILILSKYLELRQFDIKRLRELFVPFAIIGIPVLLIYLQP
                     DFGTAMIYVPIFLTLLFLGGADIPQLISIVGIAAIALFVPMMLTYLEWSGVSISGGIF
                     SFLKGNELLYIISGSLLIVSIITFILYFFFNSKIYRAIYIPSFIFSSGLFLSVIIQRF
                     LKEYQKRRILVFLNPDLDPYGSGYNVIQSKIAIGSGGIIGKGFLKGSQSQLGFLPEKS
                     TDFIFSAIAEEWGFIGATIVLLLFAYIIYKGFTIALKASDKFGMLVAGGITCMIFFHV
                     IINIGMALGIMPVTGLPLTFLSYGGSNLIMSLISAGILININSRTR"
     gene            complement(32172..33998)
                     /gene="mrdA"
                     /locus_tag="PLV81_01350"
     CDS             complement(32172..33998)
                     /gene="mrdA"
                     /locus_tag="PLV81_01350"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOM08780.1"
                     /translation="MQFASIRTKIIEEFRKRLFFVGIIVIAMFGIFFFQLVNLQIIQG
                     KEYSEKSRMNMENNIPIPASRGEIYDRNFAPDKQNMVLATNRPSFNIIVIPARYQEKQ
                     TLYYAIKNVCALLKLPYDEIIADIQSNNPYNRYIIQEDVPFDIVVTIATNQDKFPNIM
                     WEDAPVRVYPFANMFSHVLGYIGSLTKDEYNTYKDVGYKYYQKIGKAGIERQYDSTLR
                     GVDGYVRRIVDAKNRTEGEEIGQHPKPGYNIILTIDYDVQKTAYTALGDNRGSVIVMK
                     PATGEILSLVSKPDYDPNLIISKNNAKIITQLNNDKNKPFLNRTIQSRYPPASTFKLV
                     TAIAALEEEKWNPNVTLYCPGKYTLKGYVDRDFYCYQAHGSVNLLWAIAKSCSVYFYQ
                     LGLKLGPTTIFNYAGYLGLAEPSGIDIPGEITGFIPSKKWKLKTFGQPWFDGDTVNLS
                     IGQGFMLVTPMGMMDFISAIVNSGVVFKPHLVKEIRSNDNSSIVSVVVPEKLREIPLS
                     PTTLETIKQGMRMAVVSGTAARLGYLKVQMCGKTGTAQTRSKRQEDASQHAWFVGFGP
                     YDAEPEKVVAIVVMVEYGVAGAATAVPVAEQVFSTLIKKGYF"
     gene            complement(34002..34502)
                     /gene="mreD"
                     /locus_tag="PLV81_01355"
     CDS             complement(34002..34502)
                     /gene="mreD"
                     /locus_tag="PLV81_01355"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOM08781.1"
                     /translation="MILMYIITGALLLASLIIQGHSSFDVLRIGGVKPDLIFIVVVYF
                     AYSFGSLYGEVTGFIGGLLHDAISNSPLGLLAFPKMALGYIIGFFGRSIFKENLFTVF
                     LLIFFASIAKGVITLILCYLFHQGSLSSITSIILPESFYNALLSPPLFFLFDKIFEQE
                     LSREGR"
     gene            complement(34499..35368)
                     /gene="mreC"
                     /locus_tag="PLV81_01360"
     CDS             complement(34499..35368)
                     /gene="mreC"
                     /locus_tag="PLV81_01360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOM08782.1"
                     /translation="MEFFIKHRTIVLFILFTLFCFISLSLQSSGVTNVVEGAGNAVIF
                     PFQKLYHAVQTNVSLLWSGFSQLRNAQEELEKTRLRLQQYESVAEELEEIKRENEQLR
                     NLLGLKQRLKYDTVLATIISKDPDNWFRTIIINRGSSDGIEVNMPVVAFFGDEKAIVG
                     KIIEVRGSISRILPIISPDLKIGVVFQESRYPGLLSGYSAIGKLCVIDYVDKTATIKP
                     GDIVITSGQGGVFPQGLLVGVAVKSFPSESGAFQRVLVKPYINFDLIENVVVIKKEPD
                     KEILKLIQDDSQL"
     gene            complement(35454..36479)
                     /locus_tag="PLV81_01365"
     CDS             complement(35454..36479)
                     /locus_tag="PLV81_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOM08783.1"
                     /translation="MIFDSVYSLFSNDMGIDLGTANTLVHVKGQGIVLSEPSVVAVQT
                     STGKVLAVGHEAKRMLGRTPGDIVAIRPMKDGVIADFETVEKMIRYFIQKVHKRTTLV
                     RPRVVIDVPSGITEVEKRAVRESAEQAGAREIYLIEEALAAAIGANIPIHEPAGHMIV
                     DIGGGTTEIAVISLGGMVIADSVRIAGDEFDEAIIKYMRTQYNLVIGERMAEDVKFRL
                     GNAFPEKKIETMELKGRDAISGLPRTLEIDSTEIRKALKEPVDQILDAIKHTLERTPP
                     ELAADIVERGIVLSGGGSLLKGLDKYISKETGVPVIRAENPLTCVVMGAGKFLEEIKN
                     LYRSNLK"
     gene            complement(36602..37597)
                     /locus_tag="PLV81_01370"
     CDS             complement(36602..37597)
                     /locus_tag="PLV81_01370"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM08784.1"
                     /translation="MYYRYKSKQRRKITTKWVINLLLIIAAVYVVHQYHQYIFFWKYT
                     INKLQEKIEIASKQPDSTIRQQQLKDLCNAAYKYAESNPLSSDAYYLLGETYCHYAYS
                     LLNKSIYSLYDESTLQNISANIRQYFLLSIKAINKGIALGGRSSIDDNTRVIQAISLY
                     LSGYYPVNEIYNIIQKVSNIQKLSLHNIRMYSLIAIKSGHYEEGIELVKAKGVSDNIE
                     GRMFLAASYLYANQQSNAIMQYRNILQNSRDNTIIKEVHIELGKIFYRKGVYDQSIEH
                     LNAALQIDAQDVQCKLWLGKVYSAVGNVSQAKALWSEVLLLDSENAEAKRLLGIM"
     gene            complement(37618..38253)
                     /locus_tag="PLV81_01375"
     CDS             complement(37618..38253)
                     /locus_tag="PLV81_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08785.1"
                     /translation="MILLYAAVASVITGVLLILYSEFFEVSIKKTIPHDRFVPNFDDD
                     VSTPGYHKTTSTLNSQTDKEDFNDLPDVDIDITNDISTRPEKDSVIKNNDKENVLQSQ
                     DARKGYEVILYVDSSGTIGSVSDNLTSHDWSFLTRVGSGYLQYVQQGINITIDNKLYR
                     YDFYRIASMKAHDNYMLFSIKGKNTVNCIVLNEESDILLNIEKEYSNFIKS"
     gene            complement(38412..39362)
                     /locus_tag="PLV81_01380"
     CDS             complement(38412..39362)
                     /locus_tag="PLV81_01380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOM08786.1"
                     /translation="MPSFKIIEQAILKREHLFKSTQTNAFRLFNSAGDSCEGLIIDYY
                     NGFVLLQLYDSSLYKDYSKICNDCKKILSSLHIPVNGILLKDRTKVNDPQQIAKLRTS
                     ILVDGEMPPENFAIKQNNIIAYVDLIQGQNTGVFLDMRKVRETMVKYYHNGGRLCNLF
                     CYTGLFSVHSLKNGLQYAVNVDISQTVLSRAKQNYVLNDIQYDSRDFIKLDCRQYLKK
                     HIKQKAHFDFVIFDPPTFSRNKKQNFSVKTHYRDYLALIEHITKDGYALTTINAISMS
                     VRDYRSLHPQHWKLEFLEHEPEDFPYKSQPYLKAGLWKIP"
     gene            complement(39363..39989)
                     /gene="eda"
                     /locus_tag="PLV81_01385"
     CDS             complement(39363..39989)
                     /gene="eda"
                     /locus_tag="PLV81_01385"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOM08787.1"
                     /translation="MQFKLTVPVIPIGVFSDSSKIVTIAEILHQHGIHCIEVTLRTTQ
                     ALACISALKKSFPQMLVGAGSVLRHDDFKAAIDAGATFVVSPSISDSLIELSHRFNDI
                     QYIPGFTTPTELAHALQLGCTILKFFPAEYAGGVKYLKAITEPFVKYSFSIIPTGGIM
                     PSSIKQYLAIPNVIACGMSYIVDSTLIDANDFHALEDRIKETLDSIIQ"
     gene            complement(40003..40377)
                     /locus_tag="PLV81_01390"
     CDS             complement(40003..40377)
                     /locus_tag="PLV81_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOM08788.1"
                     /translation="MIVIENVDHIGITVSDLDRAIEFYNDLFDFEVVERVSNSSEAYI
                     KVGDILIKLVEVKDYKAPDNSKSHICFYIDEEDFDDALDELEEKNIPIVFGPENIRKG
                     RLVVFADPDGNKIELRYPRLLL"
     gene            complement(40528..42357)
                     /gene="glmS"
                     /locus_tag="PLV81_01395"
     CDS             complement(40528..42357)
                     /gene="glmS"
                     /locus_tag="PLV81_01395"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOM08789.1"
                     /translation="MCGIVGYIGNKNVAEVLINGLRRLEYRGYDSVGIAALNNKLIVQ
                     KQKGKVSELNAILNYDDFKGFSAGIGHTRWATHGEPNDTNAHPHTDCSGEIALVHNGI
                     IENYAAIKKILLQHNHTIKSETDTEVLVHLIEYYHQTMPLVDAVQKTLTKVEGTYGIV
                     VISKKEPDKLVAARNGSPLIIGVGDGEMIVASDASAIVEHTRNVIYVSDGEIIEITRN
                     GVKTFDLKKQTVEKHTDKIDWDIADIEKQGFAHFMLKEIFEQPETIQNAFRGRILLDE
                     GKTRLDGLRLTEDELNAIRRIIFIACGTSWHAGLIGEYLIEEYARIPVEVEYASEFRY
                     RSPILDKGDLVIVISQSGETADTLAALREAKARGVKVLGITNVVGSTIARESDGGVYI
                     HAGPEIGVASTKAFTSQITVLILLTFYFARRKYMTADAGREFLQELINAPQHVKQILD
                     KNDEIKKIAQLYKEHRNFLYLGRGIQFPVALEGALKLKEISYIHAEGYPAAEMKHGPI
                     ALIDENMPVVFIAVKDEVYQKVISNIQEVKARKGKIIAIATEGDEDIKKYADHVLYVP
                     QTRKIVSPLLTVIPLQLLAYHIAVLRGCNVDQPRNLAKSVTVE"
     gene            complement(42786..42956)
                     /locus_tag="PLV81_01400"
     CDS             complement(42786..42956)
                     /locus_tag="PLV81_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOM08790.1"
                     /translation="MAYKITEECTMCGTCAAECPSQAISEGKDRYVIDQDACTECGTC
                     VDVCPAEAIVEV"
     gene            43344..44336
                     /locus_tag="PLV81_01405"
     CDS             43344..44336
                     /locus_tag="PLV81_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08791.1"
                     /translation="MKYISKISIVLLLVFTCSAVFAGQWVYKPMSINAQKGDIILSTS
                     PGFIMDLLAILGCYWSHSGMAVDNGFNIRHNTMYVSEVPIEYNYIWFIKTTPKRMDPT
                     RLSNGLPGILTEDIDTTYNVTKNFHAAGGAVLKPTAANEALYRQYLQLAADKLLYVKA
                     YYRVNAYVNMYQLDYVNYYITGRGNHCSGTCWYANYFAGKTMNVAYIPPSLVTQCAYN
                     LYNSVKNMVRDEAGGFGAFIIDIEGLFGTGADEKIANQIVNTFGWDRSWDTSSYWRTY
                     INQKSATANAPDHLLLYTYTNPSGYNPGVQTTSSSYYGQVDPLVITSGYYYWVD"
     gene            44484..45749
                     /locus_tag="PLV81_01410"
     CDS             44484..45749
                     /locus_tag="PLV81_01410"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOM08792.1"
                     /translation="MKRTYIITGIIIFCIAAFVLILSVNKSNQQDDTSSKTQSLLNVF
                     QFMSAPEPQEEIPPVDPEKVETYEIEQANFIKEIILEKNPVCAGEDFKVTVIAKNPFG
                     PDAHLVYRISNKLGNPAILRFTKAGLREFYVTVRDEGKHIDMRKVQINVVDCADKPII
                     TLSARLHNLEPESAIFEVTSQKGLTGKCTYDWDFGDGTYATTQTGYITHSYASRDQKG
                     LQSSFIAQVKVTDANGISAIGRGSISFPNVHYLSNLMGNPVIPVLYNKFPSIDNAYIT
                     VEINMKNIFDEPVAFNNAVIECKPCNTALSSDYKNINPDQILESTFIAPKSSVKQLLK
                     VPKSLLPKSTCNLNITLSGPYPDKREVYAKIFLNIPPQSKDDIDTTRDKIITDDEMIQ
                     KLNKAAQILGKDRPITPQDIQKLEREGKL"
     gene            complement(45755..>46289)
                     /locus_tag="PLV81_01415"
     CDS             complement(45755..>46289)
                     /locus_tag="PLV81_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08793.1"
                     /translation="SLLQQSKDIQVRAELHRTDVIRFHMLYNPSQFDTKEEVHEGDSK
                     FDKKLVAVDLVLSPTSGKKVSQLQKGDFIYVLIDTSTPYGYDVAKTLNLIVDNKNTPI
                     EAEVYSVNYNKKKGYTLFVKITESLYGKAIEEQDIKVKTRLAVSDEEATSSKASLVIG
                     ILAGLIVIGILLFILLA"
     assembly_gap    46290..46389
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<46390..47018)
                     /locus_tag="PLV81_01420"
     CDS             complement(<46390..47018)
                     /locus_tag="PLV81_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08794.1"
                     /translation="MSAKTLIAIKSKIYCAKLNISGLLLTVIDKENNRIKGINGVFIA
                     GDNLKNARTNVSSIDFIEAINVANQQYAVQKEYFEVIKNYLLQRVDQKNITVLLNNIE
                     NNPITAVNEVQRIIKSALNIDIVDVYSEFDSISNAQYTEIIITQKIDSLTLSQQVEIV
                     APYYDNDRQVAEESIKKGKQDIGIAMLYLDSSQFSGICFCFVNCATRKL"
     gene            47199..49484
                     /locus_tag="PLV81_01425"
     CDS             47199..49484
                     /locus_tag="PLV81_01425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HOM08795.1"
                     /translation="MKKIMPKLKNRILEFKNFIIQLINQIYRGEEAFNWLTTKIINMI
                     KVFIVASRKFMKDDCLTKSSSIAYTTIVSLIPTLAVVLTIYSIFSGVGDKKDELFRQI
                     TLFLVEHNIKINVDPFFQTISTLIDNAAKIGGIGAVVLAFSATAVLRTLEKSLNDIFR
                     VTKQRSMFLKVIYYWAALTLGPIMIIAATAVATQISNTLSAPHLQAIAHYNNTHYIVG
                     NKATILFNNKDDLNFTSMPIEKIDFDNQHIFSYNPNDKQFNAEEFRIEIIEYKRTKFS
                     DIAFAGQKGFIIGDNGIILKSYDAGKTWLIEKWGNFTFNDIEMIDDNTGFIAANNGYL
                     LKTNDGGRSWQVMEWENVTSNFNAISFYKDKGIIVGDRSYILTTSDKGKTWQIQQLAE
                     GKYKKNFLNFNDVQIIDTNTSIIVADEGFYLISSKNFTSWTAHKFKDNNLYAVYFINQ
                     NTGFLAGEKADIYKTIDGGEKWTVKKLKGERINELSLNNNLLWAIGNNSFLMLSKDMG
                     VTWQGLKGSSFFVYLLNFFAPFFFIWILFLMCYMILPNTKIPLRPAAIGASFTSAVWV
                     IFILLFIVYIKAFAKSTFAIYGALAAFPIFLLVVYSSAVIILYGAEISYVLMNPLSYK
                     YLNRALKDKKDIHVYSAIALLHHIYKKFESGKGASSFQELLPMTLNDVDLLDMLLDLF
                     IKEKFITHTEEFGIIPANASKNISIAKIIDTIYSISLDIPVTKQDKLKTYLADLFKDM
                     KQSLDSIVKNKTLADVIQATE"
     gene            complement(49495..50376)
                     /locus_tag="PLV81_01430"
     CDS             complement(49495..50376)
                     /locus_tag="PLV81_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOM08796.1"
                     /translation="MERAEIRFDLVLLVSFFMHTIVIVSFLIAQIRYGAFAKQQADSL
                     FKGRDIIVNINQDDVKDINKNTLLSDKTSKAKGYITKEKGDRWLNNSLDFKMQKGVRQ
                     LGKGGIASSTAAKDKIMVAQENTEYSITFFKDEAGGVPEFEGDKEFTRIPDKYSITPK
                     NALFFTSDGFFSFNTVKFKPFEYFKNMKDKVAANWHPPLLANAVIYGYNPMTGSMAPG
                     RMRIMAVPTQDVKLYFTMDREGNVLDIVLVDSLGNKAIDESCIDAIRLSKNFGKVPDE
                     IQGKVIVIPFLFRIVSQ"
     gene            complement(50358..51608)
                     /locus_tag="PLV81_01435"
     CDS             complement(50358..51608)
                     /locus_tag="PLV81_01435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HOM08797.1"
                     /translation="MKIIIFEDSQYNDFFPVTLTRPVWDIRCGIFTIHYRYATLFPSQ
                     FPDSIYFYTRHHLEAIAHMQYPQLHINEPVKMNHSEELLFINARVIYPELSDLHDNTI
                     YMHNGIPVLAKLHEAAIPQFTTTEDVQNFLQNKAVVENYKGYVLTSLWELIGNNGDII
                     TQDFKLIKNPRGNNSVTILGDSSQLIVEDDVTIEPYVVIDCTKGPVYIGKGCSIKSFT
                     RIEGPCAIADNTILLQAKVREGCSIGPWCRVGGEVEECIFQGFANKYHEGFIGHAYIG
                     SWVNLGAMTTNSDLKNNYTTVKLFIGEEKINTGLLKVGCFIGDYTMTSIGTLINTGTV
                     MGPGCMVIHNGAITPKFIPPFSWYIDGKIVDLPSEDKFFATCKTMMSRRNVEFTAEHR
                     KLLEEVRSQTKIYRKGIGRWNVPK"
     gene            complement(51845..53959)
                     /gene="fusA"
                     /locus_tag="PLV81_01440"
     CDS             complement(51845..53959)
                     /gene="fusA"
                     /locus_tag="PLV81_01440"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOM08798.1"
                     /translation="MLKEYSTENVRTVAIVGHGSTGKSTLFDAMLFIGGQIDKIGKPD
                     DGSLTSDYDEEEKSRKISIRSALGFVEIDDVKINIIDTPGMSDFVGEARAAIQVAEAA
                     IVVVDAVDGVQIETEKVWRYLESKKIPRIIFINKMDKERANFNAIIDNLKSHFNAKFA
                     AVCIPVGEADKHSGIVDLIEMKAMMPKPDGKIQVSDIPADLKKAAEDARNSLMELSAE
                     GDDALIEKYLEGEPLTEDEMKKGLKALVARAEVFPVICGSSLKAIGIKNLLNVIKNYV
                     PAWELNKQVEGLNPNNKEEKVVITSKPDAPFAAVVWKTYIDQYAGRFNYLKIISGTLL
                     PETEVLNSTKNYKERVTKLYTMIGNKPVEVSKLLPGDIGVVVKLDRTATLDTLCDTKH
                     SVLLPIIQLPNPVFSYAVQASKKGDEDKIGQIFSRITDENPTITYIFNPETKQTVLSG
                     MGEMQLDIILKSIKEKNKIELVTSEPKIAYRETITKKAEAQYKHKKQTGGHGQYGEVF
                     IRVAPLERGKGFEFIDSIVGGVIPRNFIPAVEKGLREGMDEGVLARFPVVDVSVELYY
                     GSYHPVDSSEMSFKIAARQALKKGLEAAGPILLEPIMEVDVYVEKDSMGDILNDITSR
                     RGKVLGMGSSDEEGKSPISVVKALVPLAEMLRYSIDLRAMTSGKATFEMRFSHYEPIS
                     GRIAEKVIEERKKEFAEEEANK"
     gene            54333..55676
                     /locus_tag="PLV81_01445"
     CDS             54333..55676
                     /locus_tag="PLV81_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08799.1"
                     /translation="MRALHHYTIPFIVLLCIHLFAVNTGSFAQYVYEASTLHNAASRS
                     QTVQQIFYQRWDTIFSNYLHFDAYIHLNWVYDFYVNSYNRGTGTTQAVPMRLVRTFSD
                     IALLFPVYTPVTETPDEAQPVDITDVSTFRKPWVDNSILLGFTACGYHYGLTRTVDIN
                     RGSAGTESASDYAFSQFFDDIVAITAVFVPYVTLHAGIVFNQQIEPNEDGTLDYNFDP
                     STITKRYFASVSLYNILTWRSTAAKDRLESYDISLNITQFLSITTTIPSYLPQITIGY
                     KKYQKYNDEPYDPVWVSTASGKSNTMSDSLKQHATLYTYYTTLSKNFGNFFLATSIEL
                     QHVDRTIVDKRTEQKVSLPVMRLWWAKAGYDLMDRPGWDFIVWLGVNKFYDPAMSYHS
                     KHSDNDTGGWFAMIDGTSPYITLSIKASYNDYSELDKLIEAIDKYIIEGSASFRI"
     gene            55681..56196
                     /locus_tag="PLV81_01450"
     CDS             55681..56196
                     /locus_tag="PLV81_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08800.1"
                     /translation="MPNKFIQILLLWAIVTANVACSYVADYIEGEITNRARFSISAHY
                     EAGTGVIVEWDANPGNDSFAGYEIYMTQEPNNEYSDYFVIGAPYDIGTSSYFQIDGAL
                     SNSSTTQFIHTSLPPSGIYFYRVGVIEWDERDYNNDGEDEKKPASPTPALYKLYTNIA
                     EISGSAMVEIP"
     gene            complement(56223..56819)
                     /locus_tag="PLV81_01455"
     CDS             complement(56223..56819)
                     /locus_tag="PLV81_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOM08801.1"
                     /translation="MARKLRVQMPGLTYHITSRCIEWRKMLEEEYFKACFVEILRKAK
                     EKYRFKLIAYCIMDNHIHLVVHTLEDGAPISRIVQYIKARFAEMYNKIIGRTGPFWNE
                     RYKDSIIEFARDGFHYLLWLLWYLAYNPVRKNLCSDPTQYRYSSIQAYLDEHADVGVP
                     IDHHEYFMQLGDSFAQRVARFLRYDEAYRKRYSIAGWV"
     gene            57425..58594
                     /locus_tag="PLV81_01460"
     CDS             57425..58594
                     /locus_tag="PLV81_01460"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOM08802.1"
                     /translation="MHYCIIGNGVAGTEAALAIRSQDSSSAITIITQSAYHMYYRPRL
                     IEYLAGDIKPENLYIYKPATYDEKHIEVLFKTKVIDIDTKNKYITLHHGSKIKYDKLC
                     IATGATPSMPSIPGTTLQGVFSVRTIDDADAIINHINNTPDKKVAVIGGGLLGLETAY
                     SLSRRGCSVTVIEFFDRLLPRQLDTDGAAIVKKLLEQKGLQFVLGDSVTEIVGDSSAQ
                     KVICKSGKQLEASTVIFSMGIKPEISLAKNAGIVVNRGIIINNYLQTSEPDIFAAGDP
                     TEYNGICYGIWPAAREQGKLAGLNMAGARQEYKGTLLSVLLKITGIDLYSAGDFNKEG
                     TKSVVHKTDSIYVKFLYNDVDPVAAIVIGDMNIIKLARNVMEKKAEIKELVKLFV"
     gene            58662..58841
                     /locus_tag="PLV81_01465"
     CDS             58662..58841
                     /locus_tag="PLV81_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOM08803.1"
                     /translation="MKTIKKHQYIIDTKGKKNAVILPLDEYEKLLEDLHDLRIIAERK
                     NEKNIDFDTIKSRLK"
     gene            complement(59129..60286)
                     /locus_tag="PLV81_01470"
     CDS             complement(59129..60286)
                     /locus_tag="PLV81_01470"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOM08804.1"
                     /translation="MSYHTMLSQRANEFTSFIVMDVMAKAEELERKGEHVIHLEVGEP
                     DFMTPKVIEEAAIEALKKQKIKYTHALGLLELREAICEYYYNEYHVSLSPDQILVTTG
                     TSPALLFAMLAIINTGEDVIVSNPRYPCYQNFIIAAGGLVNEIVTYPEEGFQYRPDDI
                     KKKITPKTKAIMINSPCNPTGIVMSKDALQTIAQFDKQFIISDEIYHGLVYKDRAHSI
                     LEFTDKAFVINGFSKLYAMTGWRLGYCIFPKEFSKVMQRIHQNFMISANNFIQWAGIA
                     ALKYAQQDVETMRQTYNTRRQYMIERLQSIGFRIHVEPTGAFYVFADARGFCSDSYKE
                     AFNIIEKVKVGVTPGIDFGSAGQGFLRFSYATSLENIEEGLNRIEKYLKGK"
     gene            60552..61037
                     /locus_tag="PLV81_01475"
     CDS             60552..61037
                     /locus_tag="PLV81_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOM08805.1"
                     /translation="MVQILILYYSRSGNTKELAKAIAEGVKQVEGAQYILRSTEEVTK
                     DDFLNSHGIIAGSPVYFGTMAAELKHLFDDYVIIRRKMENKVGAAFATSSDPSGGKET
                     TLLSILQAMLIYGMVIVGDPMEATGHYGVSCTGAPDEKTKKNAALLGKRVAELAKKLW
                     D"
     gene            61041..61727
                     /gene="ispD"
                     /locus_tag="PLV81_01480"
     CDS             61041..61727
                     /gene="ispD"
                     /locus_tag="PLV81_01480"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF001183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOM08806.1"
                     /translation="MKHYAIIVAGGMGQRMGTAVPKQFLQLGKKPIIAWSIETFAASP
                     LIDKIIIVIHPDYKQQLEDVIHTYFPDTDIIITHGGQTRQESCFNGLQAYDFSNDDIV
                     LIHDAARPFITDDMIKQCIEIAALYGACGIYVSVKETIAQVNDSTVTHILPRETLYAA
                     QTPQCFRYSIIMDAHQKAREHNTTNATDDVSLALLAGYTVKAITGDFYTNIKITTNED
                     MELAKLLIKE"
     gene            62154..62984
                     /locus_tag="PLV81_01485"
     CDS             62154..62984
                     /locus_tag="PLV81_01485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007341396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOM08807.1"
                     /translation="MINILDKYNKPIFFYGLSLIIPWVLWFIVAYFSHLENQGPIVFL
                     IPSLGILGLISPVIVAGYLFHSDRELMEDLKSRIFNLQKFNLFYFAITFFLIPLSIVI
                     AQLISVLFGHSLSQFYISGKPSFNSFIFSPWFILIFAPIVEELAWHSYGTDTLRRKFN
                     LFNTSIIFAIYWVFWHFPLAFIKGYYHSNVVAEGLLYSFNFIASIFVFVLLMNWLYYK
                     AKRNIFVATIFHLIANVSNEIFATHPDSKVIQTVLLLIITIILLVKDREMFFNRAIIE
                     "
     gene            62999..63700
                     /locus_tag="PLV81_01490"
     CDS             62999..63700
                     /locus_tag="PLV81_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08808.1"
                     /translation="MMKTIENLLKNSGLPGPRGNLELLYSFSENATKNEVEECLSFYK
                     DDLHNSPEEFVVMCGIVGYCILNKNDIKKTLATIRKYASHSSWRIREAVAIGIQEITE
                     HNTSEIINNLENWTDGNDLEKRAVVAALCEPKLLKENSFVIKLFEILYKITMSFDKIN
                     GKLSESQNSLRKTLGYGWSVAIVSMPDQGKLAFEKIARCNNKHIKWIVKENLKKKRLT
                     VMDKKWVNKLRANFV"
     gene            64358..64834
                     /locus_tag="PLV81_01495"
     CDS             64358..64834
                     /locus_tag="PLV81_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08809.1"
                     /translation="MNGADYKHSEKNYFDLAMCIGGEWIFNGFKNTLSYLLQNVKQDG
                     MIIAGCPFWQKEPSEEYLKTVELKKDNYGTHYENMLIGKELGLELIYSIVSSKDDWDN
                     YEGLNWYASFKYINANPFDKDNQELYNRIIKQQDSYLKWGRETIGWAIYIFQKVKI"
     gene            64977..66122
                     /locus_tag="PLV81_01500"
     CDS             64977..66122
                     /locus_tag="PLV81_01500"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HOM08810.1"
                     /translation="MDIKTFLILGGYGNAGRVLAQLLLQETNVRLVLAGRNLDKAKAA
                     ADQFNNLFEGNRVTGVFADASDLGKLRQTFKGIDFVVVASSTAKYTKEIARTALEAGC
                     DYMDIHFGPKIYATLYSLVEAIKNADRCFITGGGFHPGLPAALIRYAGQYFDSMEKAI
                     VGGVMNINFKEYEFSESTKIEFVEEVIDFLPFIYKNGEWRKINIMSTKDYVAMDFGSE
                     FGKRQCAPMFFEELREIPLIFPTLNQTGFYIAGFNWFTDYFVFPFIFISLKLFPKIII
                     KPMSQLMQWSLSTFSKPPYGLVVKLEAIGEKNGKLKNIEIQLSHKDGYYFTAIPVVAC
                     LLQYLDGSIRKPGLWLQANIVEPNRFMHDMKRMGIDVKVQDKNGNGR"
     gene            66279..66716
                     /locus_tag="PLV81_01505"
     CDS             66279..66716
                     /locus_tag="PLV81_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOM08811.1"
                     /translation="MKRSQVAIAKFTKDYNCAQSVLFSFCDYLNCDKDVALKISCGFG
                     AGMGRKEEVCGAVTGGIMVIGLRYGRGEKGDSSFTEETYKKTREFMDKFKEKHGTFIC
                     RKLLGGCELTTPEGQAEFKEKDLYNKVCKQCVSDAVDILEKVI"
     gene            66858..67175
                     /locus_tag="PLV81_01510"
     CDS             66858..67175
                     /locus_tag="PLV81_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08812.1"
                     /translation="MNELELQLPTLEYKKSAEDFKGEFFENQETVINGSALFDQMEYE
                     QWLIHNTNNQEGNIVNNNWVPATTFFAVRKNDLKIVGMIDIRHNLKNDFLAQYGGHIG
                     FSV"
     gene            67197..67415
                     /locus_tag="PLV81_01515"
                     /pseudo
     CDS             67197..67415
                     /locus_tag="PLV81_01515"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(67772..68476)
                     /locus_tag="PLV81_01520"
     CDS             complement(67772..68476)
                     /locus_tag="PLV81_01520"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOM08813.1"
                     /translation="MNSVIDTVTGLDVLHPVTVLVRIMCAIIAGFCIGFERERHYQPA
                     GLRTHMVLSLGACIIMILSYFIPYVYSPGSGDPARLSAQVISGIGFLGAGAIFKYGFS
                     IRGLTTAATIWTTSGIGMVFGAGFYFLGAIATVLLVITLQVFEKVELWLVEQKKIRIL
                     QITYYSDAIAAKQILAAVKTHAGDIDIRQVSITEDVENHTSDLIVHCRMDEDFSIRLL
                     FDEIKQLGHIKKIKID"
     gene            complement(68460..69851)
                     /gene="argH"
                     /locus_tag="PLV81_01525"
     CDS             complement(68460..69851)
                     /gene="argH"
                     /locus_tag="PLV81_01525"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOM08814.1"
                     /translation="MKNKKLWGGRFSDTTSPITEQISASLHFDVRLYKHDIQGSIAHA
                     KMLAKQGILTQDDCNAIIKGLQTIQKEIEEGTFPFNEKLEDIHMNIEARLTELTGDAG
                     KRLHTARSRNDQVITDVRLYLKDEINDIQKLCVDLIQKLVKKAQENKHIVMPGYTHMQ
                     IAQPVRFSHHLLVYAWAYLRDLKRLHNAFNATDILSLGSGALAGVNYTTDRHYIAKEL
                     GFGTIAHNSMDAVSDRDFIADFLYFAAMVGMHSSRLAEDLIVWSTAEFNFIKLSDSVT
                     TGSSIMPQKRNPDIAELIRGKTGRLYGNLISLLTVLKGLPMTYNRDLQEDKEPLFDSI
                     DTVKLVLKGMAEMIEHLTVNEDITKKALYKNFSTATDVADYLVMKGIAFRQAHEIVGS
                     LVKYCEQNNKDFFTLPLEELKKFSEKFDFDIYEYLDPEQSTERKKSYGSTSLESIENQ
                     IKLLKEQLHEFSN"
     gene            complement(69992..70978)
                     /locus_tag="PLV81_01530"
     CDS             complement(69992..70978)
                     /locus_tag="PLV81_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08815.1"
                     /translation="MQLLINDHPVDFTIEKESTVKDVMNSVRNWASQRSLIFSGAIVD
                     GVMYQPDSAPDTPIHDITNINCYVQSVGDVVIDSIKEGIAYCNRAEQYLKDKGSLEQL
                     TDTEFTQLNNGIEWIVSVLQSVLHMLGLERENIRFKDMTVQQYIDSIKSLQHMIKDNR
                     TNSDINKIHELFHDVTTLFRVLLCSDALKHLVIQSVDSPDVLFKTLLDLKAMVSKQVD
                     NLEAIAGLYQKGKDNEALEIIHTLVDFLYLYMRTCYQVSPVFGINLIDIVHNDQSLEE
                     LNIRLQDMLSEIVGIMENNDIVSLSDILEYELKPLIGELDNYLDIILQNIKS"
     gene            complement(71002..73023)
                     /gene="fliD"
                     /locus_tag="PLV81_01535"
     CDS             complement(71002..73023)
                     /gene="fliD"
                     /locus_tag="PLV81_01535"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOM08816.1"
                     /translation="MPVTLGGVASGMDTDQIIQKLVEVEARPIIQWQEEKDTYSRRKE
                     ALRQLQTKLNTLNNATKDLYGFRSPFREKGVQVSDTSVLQAQASRYAVNGTHTLEVLN
                     LASTHKVSTDQIKEDEILPAGTFSIEVDGNSYTIKFRGGNLRTLSDKVNEIASSIVSA
                     ATIKTIDDKSILTIESKVPGKKGEIKIRGDEEYLKKIGLVKGEKGAAKESQRIIFDTK
                     YFTGYTGDKPVEDEDGTLTVDKDGKKITIDAKLWREYILPVEYKAQKQSILELNVTYS
                     KEKSEEEVIPYKIEIGPEETTVIKGIELKGYNISRERPLEKKPKKEVEDIIGVGVVSV
                     EDGKRTEKIYKLDHNKQGKQEIPIGQDFADKKITKILFYCNDGKATFQDASLVTEIKG
                     EGILDPKNVIAEANDARIKLDGVEIVRSKNNGIDDVLKGVTFNLLSTSTKPVTITIRQ
                     NTETAVEKIKKFVEAYNDYLTFNNELTKAVKSDKVGDYQKDKNKNGIFMGDMTLLRLQ
                     NALKTAISSAYPSNVEKPIKMLYQIGVSTGEINAKWETIREGKLIIDEAKLVEVLSEN
                     PEGVQNFFGSDNDGDNRIDNGLAFKIESILDPYVSAGKNIIVAKMDLEDASIKATDER
                     IERKQEHLKAYEQKLRQKFAAMEKAISGSKSQSSWLDMQMKNLQKQTEK"
     assembly_gap    73132..73231
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<73232..73859)
                     /locus_tag="PLV81_01540"
     CDS             complement(<73232..73859)
                     /locus_tag="PLV81_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002764028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOM08817.1"
                     /translation="MRINHNLSAIFANRQLKIVSSRMDKSIERLASGQQINRAGDDAS
                     GLAVSEKMRTQINGLFQAERNAENGLSFIQVAEGSLQQINDILQRIRMLSVQAANGIY
                     SNADRAQIQVEVSQLIDEIDRISTSAEFNRMKMLTGIYARNSRVGSIFFHIGPNQGQR
                     IRAYIATMSAKALNLYDGNRKRSISTVGQANAMIGYIDAALDKLNRQRA"
     gene            complement(74177..75025)
                     /locus_tag="PLV81_01545"
     CDS             complement(74177..75025)
                     /locus_tag="PLV81_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002763946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOM08818.1"
                     /translation="MIINHNMSAVNANRTLKFTQWSVNKSMEKLSSGERINRAGDDAS
                     GLAVSEKMRTQIQGLRQAERNTEDGMSFVQTAEGYLAQTAQIIQRIRVLAVQAANGIY
                     TNADRQLIQVEVSALVDEVDRIASQAEFNRFKILTGEFSKINPKSSMWFHMGPNANQR
                     VRVFIGTMTAQAFRMKDATGKVAITLTSPGGANATIGVMDAALQKLSKQRADLGAYYN
                     RLEHTAKGLMTAYENVQASESRIRDTDMAEEMVDFTKNTVLVQSGTAMLAQANLQPQS
                     VLRLLV"
     gene            complement(75022..75150)
                     /locus_tag="PLV81_01550"
     CDS             complement(75022..75150)
                     /locus_tag="PLV81_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08819.1"
                     /translation="MSPDTSLEQGSFVSTHPYTALSARCGVINGREAKTNVQRSVK"
     gene            complement(75393..76607)
                     /locus_tag="PLV81_01555"
     CDS             complement(75393..76607)
                     /locus_tag="PLV81_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HOM08820.1"
                     /translation="MGSLSFLVKPVSYHCNLACNYCFYKRVVDLYPGSLQIMPFDVAE
                     TVIKAAIGTGSTYISFCWQGGEPTLAGLDFFREIVTVQNRLKTDVQIIENSLQTNGIL
                     LNDEWCEFLSRNNVLVGISLDGPEVIHDTYRRDYAGHGTFEKVMHAIALMNEYAVQYN
                     ILTLVTNANVTHARELYAFFRHNNFAYLQFIPCFEHDEKGNIMPYSIRGKDLGNFYCE
                     LFDAWYQDGFPYVSVRLFQDLLMYSLGAKTSCCWLGECNSYIVVEHNGDCYPCDFFVY
                     PEWHLGNIMNYRLQDILNNEKRNTFAQLKRETTPECRMCSINNFCMGDCTRYRFTTDI
                     HQRGTSKLCEAWKMLFEHYQHYEKELAERVSKLQKSVQTGKFFQLQRNDICPCGSGKK
                     FKKCCGRAPLAI"
     gene            complement(76619..78217)
                     /locus_tag="PLV81_01560"
     CDS             complement(76619..78217)
                     /locus_tag="PLV81_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOM08821.1"
                     /translation="MLSPRTVSRREMIRLSATTLLSLSPLSTISCGRRLIPVDTDIDP
                     DIYEEKNTVKAAEVISIKEKKNVPNIIIILTDDMGYGDIGCYGNKAINTPHIDRMARE
                     GMRFIDFYCASPLCSPSRAGLLTGRYPLRCGITFPLQPGKDTFMRKIVKELGYMFGAL
                     GVVDLHGAKNLVNGLPHSEITIAQALKLRNYATCAIGKWHLGDFVVDETYHPHNYGFD
                     KFVGFNASNDDWPVSYFKDKKMIIEDIGLEQQPYTGLFTQEAIQFINENHKKPFFLYL
                     AHKDPHQPCFPSKKFEGTTKAGPHGDTVAEVDWSVGQILQCLRKNNIDSKTLVIFTSD
                     NGPWFDGSTGGLRGRKGESYDGGFRVPMIAWWPGTIKERTVCNQPVMNIDFFPTVCNL
                     AGLTLPKDRIIDGKDLMPLLTGKSKQSPHDALYFFHYREIEGIRSGRWKYIRYNNTRG
                     WPVPLDKPDTFFGSAAGGHDYEPPGSNQKVPTLATWPVLYDMEYDKSESYNVIKRYPA
                     IGKKLHEKLLAFEKEFYANIRGWK"
     gene            78545..79672
                     /locus_tag="PLV81_01565"
     CDS             78545..79672
                     /locus_tag="PLV81_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08822.1"
                     /translation="MTLDIQLVENSKSTMFPHFINIPHMIYKNNDQWVPWFHNEMKLF
                     IDKKHPLFLHSDGDFFVAMRNNSPAGRIFVFENTMYNKTHNMKSAHFYFIDFYDDREV
                     ADCLFSAAIDWAKKRHCNSLLGPMGFGGVTGGGLLIDGFEHRASMTMMMYNHPYYRVH
                     IEAFGFTKYLDNFSFYLPSTAQLPDGLKQTADALLAKGEFKVLKFKSKKELQKVADDV
                     IHVFMKTLSDHAGNYELTKPECDYIVKSLLQVANPELIKIITYKGTIIGFLFGFHDLS
                     AALQKADGKLTPLSIYRIIREYKKTNWILVNGMGILPEFQGLGANAVLYAELEKTIRQ
                     YPQFKHLEMVQIQETTSKMLSNVKTLNGRTFKTHRMYQLPI"
     gene            79831..81318
                     /locus_tag="PLV81_01570"
     CDS             79831..81318
                     /locus_tag="PLV81_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009303608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOM08823.1"
                     /translation="MKKLKVFCTIVLVIVALAMPLVCTGALYAEAAHPAVTAESAETS
                     HGGHDIGTELPLWSVLPFAGILLSIALFPLFAPHFWHHHFPKVSAFWALVFAIPFVIY
                     YKGIAAHEILHIYIIDYIPFIILLWALFTISGGILVRGTLKGTPLINSIILIIGSLLA
                     SWIGTTGASMLLIRPILRSNAWRKHKVHTIVFFIFLVSNIGGSLTPLGDPPLFLGFLH
                     GVPFFWTLTHLFTVWGFAVIILLLLYFIIDTYFYSKEDKSKEEYGEKQALKVEGWHNF
                     IFLAGVVGGVLFSGLVKLGEVNILGVHQSIENLIRDAVLIIMGLLSLKTTSQQIRKDN
                     EFTWFPIQEVAYLFAGIFMTIIPALAILKAGEHGALAFLINAVKQPAHYFWATGSLSS
                     FLDNAPTYLTFFNTALGSFYAGMPEIEAVHRLIAEQNVYLQAIAAGAVFMGANTYIGN
                     APNFMVKSISEEAGIRMPSFFGYMFKYSIPILIPVFILVTYVFFR"
     gene            81392..81976
                     /locus_tag="PLV81_01575"
     CDS             81392..81976
                     /locus_tag="PLV81_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08824.1"
                     /translation="MAKTFYEVVLEGHYNFVYGMLEGYKLAAGKNFIYYLSQQVNVKA
                     TTLSEVLKEFLTLKSKLQYVIIEANSWKKFEAAVKKQPEHSIINKKYIKSIKKIESAS
                     FEFNFECYAKKYGDEIKGIFKSLPKSVKLIDYAPKEEIHKEAKGTELYAPDHEYSFKG
                     KGKLVGDIETIITFNKKFDEHPLIDVSAISLNLK"
     gene            82136..83092
                     /locus_tag="PLV81_01580"
     CDS             82136..83092
                     /locus_tag="PLV81_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08825.1"
                     /translation="MKKTLAVIIISIICIASTIMYSKYRMSSFEPQYIGFLTVENSTI
                     IKQLDTVLQKTNIAGIRSDKELAKCLEEQIKSLQQLALIAITDNEYSVLIAAKKDRVI
                     NQKLFDAIITDISQKKLLPSTTFPQITRYYSNNKYYFFIYNAPQYTIITTYSFVLPKN
                     LIIKLALENLLIVIMFIIIGTAIIITLQQKAKEPVTMKVDKTEIKPQTPDPVGSLCQQ
                     FSIISVTIAQQNKNGNLKTIYSHPDKKQNLYLQPEIINELLNGVHILAEKNTVLIFYN
                     ITTKLFFRLAKHQAFKGKTINDIERFIDNNNDVLLKFLKIKK"
     gene            complement(83110..83664)
                     /locus_tag="PLV81_01585"
     CDS             complement(83110..83664)
                     /locus_tag="PLV81_01585"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOM08826.1"
                     /translation="MQKHEHKGKSSSVYINAHELIAKLPITNGMHILDAGCGDGYFSI
                     ALSEKFPDSTIYAMDYNEQAIDNLNIVVKEKRLTNMQPMPGDITKDIPVKADAVIMIN
                     VLHGLVYNNEIDAAMKNTIQSLNDKGLLIIIDFYKREGIPGPSMDIKLSVDQATATLY
                     QRGFKIVNILQAGQYHYAIVATKR"
     gene            83883..84617
                     /locus_tag="PLV81_01590"
     CDS             83883..84617
                     /locus_tag="PLV81_01590"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOM08827.1"
                     /translation="MKIYKNISIIYEDNHIIVVEKPAGMLSQKDYTDTDSLLEILKEY
                     IKTTYKKPGNVFLGLVHRLDKQVSGIMVYAKTSKAASRLSSQFRSHDVIKIYCAALQT
                     ITSTDTAWHTIHDAIIRHHDKTLISSDNITAMHASLKYKIIYTKPSYSLALIQLYTGK
                     KHQIRAQMSHLGMPIANDIKYGATLISSETAIALHAVYLQCIHPVTKERISFYSKPQG
                     IIASIFNTIDVDHVIKNALIHEGYSL"
     gene            84638..85459
                     /locus_tag="PLV81_01595"
     CDS             84638..85459
                     /locus_tag="PLV81_01595"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HOM08828.1"
                     /translation="MIISASRRTDIPAFYSQWFIDCIQKGHCSVTNPFNPNQRKEVSL
                     LPSDVDAIVFWTRYPVPLMKYLGQLTDSGYRYIFLYTITGYPSWLEPHSPELSHAIQA
                     FKDVSRILGSQKVVWRYDPLVFSKELDFDFHIKNFAAIAQQLQGYTQRVIISIMEPYK
                     KVTRRLKVLHEKGIVTYPMNIFGQDELFKFFTCITHIAFDCGMQIHSCCSNLTEFGIE
                     NGACIDGNLINSMFGLDLHFEKDIYQRTNCLCAKSVDIGTYNTCKFGCLYCYASR"
     gene            complement(85484..86014)
                     /locus_tag="PLV81_01600"
     CDS             complement(85484..86014)
                     /locus_tag="PLV81_01600"
                     /inference="COORDINATES: protein motif:HMM:NF039195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HOM08829.1"
                     /translation="MRRKYLLCISIIIFSVMILPGCSRKEKGRFYSDEYNFSIVFPKH
                     WEIQQQEMGAVVVALSPEEGMRDDFRENVAITVEEVSGDSTLDKYFELSQKNLKSYSK
                     DYRLLYKNNTWLARKPAMILGFTYTMSGLKLKVLQYYCLNNNKVYVITCTALPHTFDR
                     YEETFLKIVRSFRFEK"
     gene            complement(86123..87346)
                     /locus_tag="PLV81_01605"
     CDS             complement(86123..87346)
                     /locus_tag="PLV81_01605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOM08830.1"
                     /translation="MFFIARAWDAITDPLMGMISDRTKSKYGRRRVYFLFGCIPFGLM
                     FMLIWLVPSHDHYILLGYYAAITILFNTAFTVVAVPYNALMPELSQNYDERTSISGFR
                     MSLSFVGNLIAAVGVSVIVDVVFKGKEHYITSYPVMGVIFGIIIIVLIFITFVGTKER
                     VQSEAVIHDGLLKTFKAVLSLHEFRIILGMFLFNMIGFDLIQALFIFFLKDVILIEED
                     MTFVVMGIPLIVAVLSAPLWIYISDKLDKRRAYMVAAIYFTLSLLLCLIAPVHNLQFV
                     IIIGVLAGVGISASQIIPFSMIPDVIEYDEYKNGVRREGAFYGITTFLYKVASASAIA
                     LASAFLAFFGYIENSATAQPQSAVSAIRYMMAFAPGLCFLISVYFVSILPITREGFDE
                     IKRILNERKSIANNG"
     gene            complement(87285..87497)
                     /locus_tag="PLV81_01610"
     CDS             complement(87285..87497)
                     /locus_tag="PLV81_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08831.1"
                     /translation="MKEGYVVPTIEKIAYGLGDLAINIAYTTIGFYFLFFFSQCCRLT
                     CAVGWHYLFYCSCMGCHNGSAHGYDF"
     gene            complement(88053..88841)
                     /gene="arsM"
                     /locus_tag="PLV81_01615"
     CDS             complement(88053..88841)
                     /gene="arsM"
                     /locus_tag="PLV81_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOM08832.1"
                     /translation="MDTNTKIKKIVQKNYGTIALSKTSCCSAGCCNPRDVASKIGYSA
                     NEMNELDSANLGLGCGNPNAIASLKQGEIVLDLGSGAGIDCFLAAKKVGQHGKVIGVD
                     MTPEMIALAKENAKKLRVNNVEFILGDIEHLPVDDNSVDVIISNCVINLAPDKERVFK
                     EAYRVLKRGGRMAISDIVLDGTLPPYIKESIDAYVSCIAGAILKSEYIALLHKAGFSN
                     VEIVKEYGYGNIFASNECNNDGTSYDGQEQYKEIVKSISLVAWK"
     gene            complement(88844..89092)
                     /locus_tag="PLV81_01620"
     CDS             complement(88844..89092)
                     /locus_tag="PLV81_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012035143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HgcAB-associated protein"
                     /protein_id="HOM08833.1"
                     /translation="MQNNNCCNSSCSIEAVVSVDERGQLVLPKDLREKYGIKAGDKFV
                     ILTLNSTESLCCIAFVKADSLNSVVFEQIAPVFSNLGK"
     gene            89461..90789
                     /locus_tag="PLV81_01625"
     CDS             89461..90789
                     /locus_tag="PLV81_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08834.1"
                     /translation="MINKIMVFIIVVYITSSSAYAFLENYNNYQYLIGDRAAGFGGAY
                     CAIANDSTALWYNPAGLANINDNRLNISANTYSYLTRNSRQYWQIEESPGQYQCLDLK
                     ETDISVVPNTVAYARRVNWLGNDVIAYGLFVPIQDSIQATIAGKATGSVLNVELNASY
                     RINTKSYYGVAGYGVNITKNLKLGLSAGVGYFQGKGGANISAYLDPGGTNQSEIAMII
                     DNELTAVTAFTGTGLQYALTPHHSIGVFYHSPIYRLHASREERTTSYQVGPIFPTNSS
                     ETTINNDDFRFRIIPAYISFGYGYISEGKWSCTVEGVVHNTNTDYPNRVINGKVGLEI
                     YLLEDLIVRTGFFTDFSQKDTVTQSSENDEKNDYYGATLSLSFGNDLNPLQSDTLKAK
                     NMWTTVGIVYQLGIGDIRGFRFYTVTSGSDPVIHQYTHRISLYIGESIVF"
     gene            90968..91282
                     /locus_tag="PLV81_01630"
     CDS             90968..91282
                     /locus_tag="PLV81_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08835.1"
                     /translation="MGILKDAGESLVNFSERFLDKTEELAQIARITMEIKKLEHSIKE
                     IYLNTGKYVYDQVVSDRPISNMDDFIIKAVATINDYKTKIQEKQNEIQKVKEHYESKY
                     HR"
     gene            91301..93391
                     /gene="rnr"
                     /locus_tag="PLV81_01635"
     CDS             91301..93391
                     /gene="rnr"
                     /locus_tag="PLV81_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOM08836.1"
                     /translation="MSKTSSRIVQAIEKQKGFFTPNDIVSAVLLSTSKSKKSKKSTKV
                     SQRQAQQTANRVQNAISTLYQCGFLTKQKGKYRVAQPFSFTGTFIHSKKGDGIVYLHD
                     DIEVHIYREDVNHARNKDIVQVQLTDIRRGTLFGRVTSIVTANKQMFFARCVKPNGKF
                     RILQLIDVPGNIQVITKENIKPGVLAIVTVKNSFIQNYQECTINALIEHEEEYDVQRI
                     VGKFSLPGAHPEYEHLENIEQNVHPHELKNRKDYRKLLTVTIDGETAKDFDDAISIEY
                     KNGVYTLYVHIADVSAYVYKDDAIDKEAYNRGTSYYLGNTVIPMLPEKLSNDLCSLKA
                     GVDRLTLSVMLQFDKHGNYRTHEIYRGIINVDQRLTYNVAQEILNSKKRSNIKAMLGH
                     AKDLAQKLKAKRLSNGRVDLNLADAEMIFDNNASIADIAFATRLNSHMIIEEFMLSAN
                     EVVSRVLRQNNVPTLYRIHEPIAQEKFIALQNFLKTLGIILKEEENVGLALQRVIDSV
                     AQKEYQQVVNFIVLKSLMQAYYGPTPLGHFGLGFKDYTHFTSPIRRYPDLIVHRCIKS
                     LIDRVQPPYSPDELAVIGEQSSKLERLAQSAERDLLKLKACRLLEERVGEEFEGIISG
                     VTRFGFYVSLLDKPIEGMVPLKFLTDDYYLVNEDDYTVIGRRLGRRFRLGDLVKVRLI
                     NVDTDFMRIDFEVV"
     gene            93403..94872
                     /locus_tag="PLV81_01640"
     CDS             93403..94872
                     /locus_tag="PLV81_01640"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002083.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOM08837.1"
                     /translation="MLTLQAKRFETISSSDTVAILCTQEQIDTNSFIPQQLSDVFAII
                     NTKTFKAKPNTYFFAALKDRPSILLVGLGSHKDITPESLRNAASVIVGACSDKSVSEV
                     HIVVPEINVTNDTARYIAEGVYLANYAFNRYKTQKEETSPLLEKAILLSDNPGQLSPL
                     LKEVSIVCSNTLLCRDLVNEITENANPLTIAKLAKKIAAQSKIKCTVYNKKDIEKMKM
                     GLLLAVSQGSKYPPQLIVMHYRGAPSKKDSIALVGKGITFDTGGINLKPSGHIETMRT
                     DMAGAAACLYTINAIAQMKLPINVYAVIPLAENMISHTAYKPGDVFTAYNGKTVEIGN
                     TDAEGRLILADALSFTEKKLKPKAIIDIATLTGACIVSFGEIVAAYLTSDESLSTALF
                     NASESTGEKIWRMPLYKEYNEEIKSDIADIVNVPAVRNAGTIIGATFLKNFIENTPWA
                     HIDIAGTSWYSKKRGYNPKNATGFGVRLLYEFAKSVALK"
     gene            94918..95391
                     /locus_tag="PLV81_01645"
     CDS             94918..95391
                     /locus_tag="PLV81_01645"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOM08838.1"
                     /translation="MYSTKELIDIAIGIEETGFYFYSTFKNKFKDESFKQIFQFLADE
                     ELRHKGVFSDMLKELKDTQGLFTEEYYSYLKAIGTTRVFANKDDIDKIAPSLHQPLDI
                     IKIAMDAERDSIVWYSELKEIYRNDAKAKNILERLINEERKHVLTLLDLKEKLIM"
     gene            complement(95552..95803)
                     /locus_tag="PLV81_01650"
     CDS             complement(95552..95803)
                     /locus_tag="PLV81_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08839.1"
                     /translation="MISTPVTIATHNRSGAGYNEVIAVVRFADGPGREMQVKTQAQVN
                     GSYGFTVSGANVYDTQGRVIAQGKPYFEAGEALPIHQCQ"
     gene            95996..96448
                     /locus_tag="PLV81_01655"
     CDS             95996..96448
                     /locus_tag="PLV81_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08840.1"
                     /translation="MNIETTTCIAHENLKLLQDYADIYKLSLHTFIINFINYVMSYKK
                     IPVKSCKRLTYRKRYESWKRVHLYLYENEYEFLMDARKVCKMSIAKVISYCIENYLFD
                     FLAALDSEDNTDNYRFSGYTFMFYLENGIQCCRFYWGPHPELVKYVMQ"
     gene            96692..98584
                     /locus_tag="PLV81_01660"
     CDS             96692..98584
                     /locus_tag="PLV81_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HOM08841.1"
                     /translation="MSLYQKVYDESINNPEKFWSEAARNIHWYKPFTKVLDDSRKPFY
                     RWFVGGQVNTCYNALDRHVEGGKGNQVALIYDSPVTDTIAKFTYKEMLDKVSIFAAAL
                     QKYGIKKGDRVIIYMPMIPEAPVAMLACARIGAIHSVVFGGFAPNELAIRIDDAKPKL
                     IVSASCGIEGKKVIEYKPLLDKAIEIANHKPEHCIIFQRPQVTSPLMPGRDIDWNDAI
                     SGVKPAECVPVESTDPLYILYTSGTTGIPKGVVRDNGGHAVALYWSMKNIYGIEPGDV
                     WWAASDVGWVVGHSYIVYGPLLYGATTILYEGKPVGTPDAGAFWRVISQHKVKALFTA
                     PTAFRAIKKEDSSGEFFKKYDLSQFKYLFLAGERLDPDTYYWASNLLQKPVIDHWWQT
                     ETGWPIAANCVGIELLPIKAGSPTKPVPGYQVHIINDEGKQLPNGTEGIVVIKLPLPP
                     GTLPTLWQNDDKFRESYLDIYDGYYFTGDGGYYDEDGYLFIMGRIDDVINVAGHRLST
                     GAMEEVISKHPDVAECAVIGVEDSFKGQLPVGFVVLKEGTTKSHDQIKNDLAQMVREQ
                     IGAVACYKTTLIVQRLPKTRSGKILRGTMRKIADKKPYTTPSTIDDPAILDEIKDALS
                     KVIVSA"
     gene            complement(98826..>99454)
                     /locus_tag="PLV81_01665"
     CDS             complement(98826..>99454)
                     /locus_tag="PLV81_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOM08842.1"
                     /translation="GVHTEMLVDSYVDLYHAGRVTGAKKNIDKYKMSYTFAMGTNKLY
                     DFLHHNPTCASYPVNYINDPRIIALNDKVVAVNNAIAVDLFSQVCSESAGIAHKSGTG
                     GQLDFIFGAFGSHGGKGFICLSSTYKDKDGSLVSRIVPTLPQGSIVTVPRSIVQYVVT
                     EYGAVQLKGKSTWERAEALISIAHPDFRDELIKKAGEMNIWTNTNKIE"
     assembly_gap    99455..99554
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<99555..100327)
                     /locus_tag="PLV81_01670"
     CDS             complement(<99555..100327)
                     /locus_tag="PLV81_01670"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyryl-CoA:acetate CoA-transferase"
                     /protein_id="HOM08843.1"
                     /translation="MGDIEKWEYEKKKITAEEAANFVKSGDRVFYGEFVLFPEAIDEA
                     LSKRVEELRNVDVRSVCYMRIPKIVEADPEQKHFILNDFHFGTISRRLYDQHLCNYIP
                     ITYHQGPRVIRKYLDFDIAFLTVGPMDPRGYFNYGLANSVTSAVVTKAKKIVVEVNEH
                     VPYCLGGNQESIHISRVDHIVEGKNLPLPQIPALEPTDIDKQIAKHLLEQIEDGACLQ
                     LGIGGLPNVVGKMIAESDLKDLGVHTEMLVDSYVDLYHAG"
     gene            complement(100950..101912)
                     /locus_tag="PLV81_01675"
     CDS             complement(100950..101912)
                     /locus_tag="PLV81_01675"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOM08844.1"
                     /translation="MKKNVLISIAVIAVLAGYLIINSLIPKNVVTLPTIKSPVTELVL
                     KKGEAIILIKQDGDSWKVNEFEGDKNIIANMVNKIKDLAITDLISDKPHYERYELDND
                     HAIRVQAKSNGQIVRDVYIGKKGSTYRHTYVRFEGDPKVYLAEDTLDSYFNKTVDELR
                     NKDIVQLDIAKIDTVKLWYKNTSLTLQKKVVEKKNEEGNAQKEKQEKLKEIEKEEQWI
                     CKEQPAVILDNNKVKEMLYTFDPLKAYSFPDVNAKTLTGKIASITVSVKGEESQKPED
                     ITLVIHKEYENNRYICTSSKSQYVFTLDEWRAKRIFKTLQDLKK"
     gene            complement(101939..104089)
                     /locus_tag="PLV81_01680"
     CDS             complement(101939..104089)
                     /locus_tag="PLV81_01680"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOM08845.1"
                     /translation="MNKRNIIDMLQNLKSNITIIYTKVIQNIKEQTKSGDTETQKSYS
                     QYILYGVIIVLINVVGLTLYFRIDLTKNSVYSLSPISKEVVSSLEEPLTIKIFFNKDL
                     PAPYNAVYRYLQDLMVEYDNAGNRYFHYEFVDVEKQKDAASDFGIYPVQIREIRNDQV
                     KFRNAFMGLAIIHGDLIEKIDSITEPEGLEYRITTLIKKMNGKIDSLLKLKNPITVTL
                     YASSNLPIPGMQNLNERVYAEVQKCNIRNYNKIQYRYIDPLQDPQGSTLAQMYGLPTL
                     RWPRFTTMEGKIVEPGEGMVGIVVECNNKFETVQILSRSIFGQYSVGDLTRLEDTLNA
                     AIDNLISINPKVGYIVGHGERDINDEQNGAAQFRKMIGDMYELVTIDITKDEIPDDIA
                     TIIINGPRSMFSEYELYKIDQFLMRGGSAVMLLDSYVEQQMQGMQMFQRPPQALPVFT
                     GLEGMLAHYGVKIGRDIVMDKQCFIAQQRGLGELTIYFAPMISEEGLSKDNEITKYLK
                     KIVFLKASSISVDETLLKENKASVQYVVKSSPYSWLQQGNINFLPWGANPPQDSSLKQ
                     YNLAVLVQGNLKSYFADKQPKIDVKTKETKGKDYVQEEALKQSVKPVKLFVAGTSEIT
                     GRNIIDEEGKSPNAVFMHNVIDYMQGNYAIPQMRSKGLSFNPIKETSDTAKSVIKGIN
                     IAGLPLLVILAGVLYMWRRTIRRRRIEEQFSSMR"
     gene            complement(104092..104817)
                     /locus_tag="PLV81_01685"
     CDS             complement(104092..104817)
                     /locus_tag="PLV81_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOM08846.1"
                     /translation="MEQFKEIMKGSTLIAKKELSGYFSTPIGYIVISVFLVIAGFLFF
                     STFFLYGQAELRAYFELLPLLFAFVIPAITMRLFAEERNTGSFEMLMTLPLSTIQVVT
                     GKILAATLFASIMISPTLVYAVSVAFVGSPDIGPVIGGYVGAVLLAAAYASIGVFASS
                     MTKNQIVAFIIGFAICIVLSLITKFLYFISPKLVSLFEYISADYHFQNIARGVVDSRN
                     IIYFASVIAISSMASIYQLEVRR"
     gene            complement(104828..105754)
                     /locus_tag="PLV81_01690"
     CDS             complement(104828..105754)
                     /locus_tag="PLV81_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013243311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOM08847.1"
                     /translation="MISVRNLVKHYGEVKAVDGISFTIRKGEITGLLGPNGAGKTTTL
                     RILTGYLQPTSGMVSVDEFLVNENPIEIKKRIGYLPESAPLYPDMMVYDFLQFMADIR
                     HIYDKEKIKDVAIQCGITEVMHKNINELSKGYKQRVGLAQAILHDPDILILDEPTNGL
                     DPNQIVEIRDLIKELGKEKTVILSTHILQEVEATCNRVIIIDKGSIVADDSTTELKSA
                     RGKDIRINCIIGGTTFEGIKNALKNISGVKNVQHVRDEGQLCYIQVLTGADVDLRPAV
                     FNCVKDSGWTLYEMNREYRSLEHVFRELTVGM"
     gene            complement(105908..106270)
                     /locus_tag="PLV81_01695"
     CDS             complement(105908..106270)
                     /locus_tag="PLV81_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010153623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HOM08848.1"
                     /translation="MDDRAIYSEHRPWGFYEVLSDTETHKVKRITVYPKKRLSLQRHK
                     KRKEHWFFVSGQGVVTLDKHEIEVSSGVSVDIDYNMLHRVENTGTHNLMYIEVQLGEY
                     FGEDDIERIEDDYGRAQK"
     gene            complement(106277..107362)
                     /gene="serC"
                     /locus_tag="PLV81_01700"
     CDS             complement(106277..107362)
                     /gene="serC"
                     /locus_tag="PLV81_01700"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOM08849.1"
                     /translation="MARIYNFASGPSILPLPALEEASRELVDYRHTGMSLIEMSHRGK
                     IYDEVHNEAIALIKEILQVPDDYYILFIQGGATLQFGMIPIAFLGPGKIADFILTGTW
                     ARKAHEDAKLIGNPHVIWDGASEKYRRIPDQRELQYTPGAEYVHICSNETIDGIQWQK
                     MPTTGNIPLIVDMSSDIMSRPVEWDKVSMLFAGTQKNLAPAGMALVVMKKELVEKARK
                     DVPAYLRYDLHAKENSLYNTPPTFTVWMTTLTLRWIKSIGGLPEIEKRRDEKAAILYN
                     AIDSSNGYYNCPVDKHSRSKMNVVWRLKNEQLEEKFVKEAEKEGLSGLKGHRSVGGLR
                     ASIYNAMPVEGVTALVDFMNHFMQRNG"
     gene            107501..108190
                     /gene="rsmI"
                     /locus_tag="PLV81_01705"
     CDS             107501..108190
                     /gene="rsmI"
                     /locus_tag="PLV81_01705"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOM08850.1"
                     /translation="MSGKLYIIATPIGNMEDITLRALRILKEETDIVYCEDTRQTAKL
                     LNNYDIHVPIRSLHTHSSKARLQSIIQELESGKTIAYLTDSGTPGISDPGGQLVALAR
                     KHNICVIPVPGASAVTAIISVCGFPATQFYFAGFLSKKEGKRKNQLQKLSHLDAIIVL
                     YESPYRIIKLLHAIAEVMPQAQILIGREMTKHFEEFIYCTARDIEDIAATLTQKGEFV
                     VAVYNAKKTYM"
     gene            108278..109468
                     /locus_tag="PLV81_01710"
     CDS             108278..109468
                     /locus_tag="PLV81_01710"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM08851.1"
                     /translation="MKYYPRTLEKTLAHYLKLFPAVAVCGPRQSGKSTMLKKQYGHKY
                     RYITFDDPIVRERFNIDPKGFLHEYNNQVIFDEVQKVPGIFEYLKMLIDANRSQYGNF
                     ILTGSSQFSMVTHITESLAGRIGVLTLLPFQFSEIPQSLQKHQIIYGSYPENVVRKYQ
                     GVHEWYAAYITNYIERDVRSLFNIGNLKDFQRLLSLLASQTAQELNMNRLANDIGVTV
                     KTIKSWISVLEASYILFTISPYYNNIGKRIVKRPKIYFYDTGLVCYLTGMHTVDILHK
                     GPLAGPMFENYIIAELKKLLVHYNKRMNLYYLRDNASNEIDVIIEDPLNRKLILTEIK
                     HTATPKPLMAQRLQKLVTLVKNHIPAYTVHGMILYQGKEDFYVHDVRFANYKHFLDEL
                     ERHF"
     gene            109664..109957
                     /gene="flgM"
                     /locus_tag="PLV81_01715"
     CDS             109664..109957
                     /gene="flgM"
                     /locus_tag="PLV81_01715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOM08852.1"
                     /translation="MVIDKIGNIKNIVEPKSTKSVGKTRSTGKSDSVEISSEAKKAAE
                     QSKIAQVVKQTSDIRAERVREIKEQIANGTYDFNDDRIIDKVAEKIAMQLLRK"
     gene            109985..111211
                     /locus_tag="PLV81_01720"
     CDS             109985..111211
                     /locus_tag="PLV81_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOM08853.1"
                     /translation="MAGIIDKKAESGFRFFMSKAYGQTIEGLWPDLYIPTAVYAQPDS
                     VTLLPSIIKLFGSRILFITSSIDFNQYNHLFMPIARSLQNNNIPFMVYDEIPENNTDY
                     IDTAVYFARKTHCDLVIGFGGINSINAAKAVALLTPNYFFAHELFTYPFLHEPIPFIS
                     IPVHPLFGFDITPCTILTDIHDGIHKTFFHNYIFPKAVILDPALAFGIDDETMARYSI
                     ATLAIATESVISRNINNITNTLSLRTIDLIFKNLPAALRESDNETARLNVMMASLMAG
                     IAFSSAQLSVCLALSLALSSLTPIDLASYMAVMLPHIMEYNLTSAPGKYVQMSKVMDE
                     DVRDITVIEAAIKAVEGVRKLALDVEIPQRLSQFDINKGIFSKVAEITLSYPFIHNAP
                     RALNRDEVETILIAAY"
     gene            111215..111964
                     /gene="larB"
                     /locus_tag="PLV81_01725"
     CDS             111215..111964
                     /gene="larB"
                     /locus_tag="PLV81_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOM08854.1"
                     /translation="MSQSALNNLLQRFKNGLVSEEDFLQELSHITIEQLGHTTLDHHR
                     HTRLGFPEVIFCQNKTPEQVADIAKALAKRNTSFLGTRATKEHYNKVRRHIKKAHYNE
                     AGKIISYRAQTLHHKPHKLVIITAGTSDIPVAEEAKETALIMGLTVETIYDIGVAGIH
                     RVFHNWEFIHDATVIIVIAGMEGALPSVIGGLVPCPVIAVPTSVGYGASFGGIAALLG
                     MLNSCAPGVSVVNIDNGFGAVYCAYRIIRSL"
     gene            111974..113137
                     /gene="larC"
                     /locus_tag="PLV81_01730"
     CDS             111974..113137
                     /gene="larC"
                     /locus_tag="PLV81_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOM08855.1"
                     /translation="MKILYCDIQLGASGDMLVGALIDMGLPVDVITTQLQSLSVKGFA
                     IAPAKVERYGIHGTQAGITAHTDTTHRNLDSIYNIIAHTPCSDTVKTNAMKIFDTLAT
                     AEATVHGISKEDVHFHEVGAIDSIVDILSFCIGIEYFSIDTLYYSTFALGTGTINTQH
                     GMLPLPAPATVKLTHGKQVMFTGLQGELTTPTAAAILVTLGTQQTQFGGTIVTTGTGF
                     GTRDYGFPSYTRTLLIEDNSNEYEVLSVLECTIDDMNPEIYPHVAELLFEAGARDVYL
                     QQVIMKKGRPGVVISVLCPFHAVDQCTQILFRQTTTLGVRAYTVNRYSLPREFATVIL
                     YGEEIRIKQSYRYQELSPKPEFEDCKKVALKTGKPLYHIMEEALKEYYKQKKL"
     gene            complement(113132..113596)
                     /locus_tag="PLV81_01735"
     CDS             complement(113132..113596)
                     /locus_tag="PLV81_01735"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOM08856.1"
                     /translation="MKTQTKDTALNSMQIVCFNIGKEEYGIEILKVQEILKLPAVTPL
                     PKSADYIMGVIDLRGKVIPIIDLGVRFGISEKGAKNKRAIVVDIKGKRIGLAIDQVSH
                     VIKLENKDIEPPPPIVKGISGKFIVGIGKVENKFVIILDIDQIFSSEELKQL"
     gene            complement(113701..>113932)
                     /locus_tag="PLV81_01740"
     CDS             complement(113701..>113932)
                     /locus_tag="PLV81_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08857.1"
                     /translation="IEYGELDELFLEIIPQPHMLIKQVMKLVVIIAVLFIMFAGYIYI
                     KNGSLPANLKGYAEEGKIAIDQLHQSYLKLIK"
BASE COUNT        35868 a        21883 c        19539 g        36142 t
ORIGIN      
        1 attaaagaga ttcaggatgg gcgtaaagac aagctgtcta tggttgtatt cagcggtgac
       61 ctggacaagc ttcttgcagc atttattatt gctactggtg cagttgcaat gggaatggaa
      121 gttgttatgt tcttcacctt ctgggcaact cctgtactca gggataagaa gaaaaaaggt
      181 aaaggtaaaa acctttttgg caaaatgttt agctttatgc tgcctaaagg ggcatcaaag
      241 gtaaagctgt caaagatgaa tatgggcggc atgggaactg gaatgatgaa atatttaatg
      301 aagaagaaaa atatccagga ccttgatggc atgatagcac ttgctgcaga attaggtgta
      361 aaaatctatg tatgtgaaat gtcaatggat ttgatgggct tcaaaatgga cgagttgatt
      421 gattacccgg atatgaaatc agttggcgtt gccacatttt tacaggaagc atctgacagc
      481 aagattcagt tgtttatcta atttttttta aaatttcttc ttgtattatg tatgttaact
      541 ttatataatt catttgttaa agtgaactaa aatttttata ttacatatgt aaaattttga
      601 aaatttaagg agtaaaacca tgagtgacat taaagcagat gtaacaatgg atttaaaagg
      661 attggcatgt ccacttcctg tggttaaagt ttcacagcaa attaagaaaa tgcaggttgg
      721 ccagattctt atggctgaaa caactgaccc gggtgcttta gcagattttc ctgcgtgggc
      781 aaaaacaagt ggcaatgaaa ttattgatac tgtgaaagat ggtaatgtaa ccaagttttt
      841 tattaagaag cttaaatagt taacaacagg gtgaggtgaa taatgctggg ctatatatcc
      901 tattttatac ttgttccaat ggtatatatt gcctttgtaa ccttgattgt agggcttatg
      961 tataaatttt atgttatctt cagggctcct gttccagctg gtacaggagc catttttcct
     1021 aaaaaaggtt caaaggtatt ggggcttata tatgatgcca tgatagttcc taacgtgtat
     1081 aataagcaga aggtattctg gtttattata atgatttttc acattgcatt tttcttgctt
     1141 tttttaggtc atcttgaact ggtagatgac tttgcgttta ttcagataat acctcataaa
     1201 atctttctgg gtggtggtgt tgttggtatt actcttataa taacaacatt atactttcta
     1261 ttcagacgtt ttggaacacc ataccgtgaa atatctatcc ctgaagattt tgtgattcta
     1321 cttgtgcttt tcttctgtat attgtttggc tcaatactgc atctggctga acgatacagc
     1381 gactggggag cagtgttgcg ggttaatgtg gatgtttacc gtgaatggct gcaaagcatg
     1441 attttgctta aaccggaact atcgtacaaa ataactgaat tgtcacatta tactatactg
     1501 gttctgcatg tactctttgc aaatatcttc ctcatgatgt ttccattcag caaaatggta
     1561 cattcagttt ttacattttt agcacattat cgtaaaagga aatagccatg gataaggaaa
     1621 aagttctgga acttaaacag gttattgaaa agaaattgaa caggcccatg ctgtactatc
     1681 tggacctttg cacaagatgt ggcctttgtt atgatacatg tcatgcatat cagggcatcc
     1741 ccaaaaagga atactcacct gttggaagag ctgaagtagt ccgtaaagtg ttcagaaaat
     1801 atttcaggcc aacagggaaa ttatttccct ggtggggcga tgcacaggaa tttgacgaaa
     1861 accttatgga agagctacga gaggctgcat tctcatgcac tggatgcaga cgctgtacca
     1921 tgttctgtcc gtttggtatt gatatgccat atatactggg aattgctaag gaactactta
     1981 ttgcttacgg aaatgcccct gaagaactgg taatgcttgc cgatggtgct gttgaaaaag
     2041 gcaaaagcgt tgatatgttc aaagaaggat tcaaaggcgt catcaaagat cttgaaaaag
     2101 aggtccagaa gcttatcaat gctccaacca gcgatgggct tatccccatg gaaaagaaag
     2161 gagccaatat actttttgtt ggcctctcag gtgcacactc tattgtgtat cctgccgcaa
     2221 tttttaatgc agccaaagaa gactggacac tcagctattt tgaggccgtt aactttggtt
     2281 tttttgcagg cgatagtgaa aaagcaaatt ttattgccaa cagggttatt aatgaagcaa
     2341 aggaattagg ggttaaggag atagttattg ttgagtgcgg caccgcatac agaataccta
     2401 aattcttaat gggacctctg ccctttaaag tatcaagcat tgtagaagtg attcatcgtt
     2461 atataaaaga aggtagaatc aagatcaaag aaggtgctat taccgaacca gttacctggc
     2521 atgatccttg ccagcttgga cgaaatggcg gcgtctatga ccagccgcgt gaagtattga
     2581 atatgctggc aaaagattat cgtgagatga acccaactag agaatataac tggtgctgtg
     2641 gtggtggcgg cggactggtt gcccttgaca atgaggaatt tagaattaag agtggcaaac
     2701 ccaagaaaga acagattgaa aaaacagggg ctaaaatggt ggtcactgcc tgtgaaaact
     2761 gccacctgca actatcaaca ataagtgcag gctatggtat gggtgttacc attgaatcat
     2821 ttactaaatt ggttggtgaa aatttaattg tttaaataaa aaaaggcggg tacaaatccc
     2881 gcctttttaa attatatctt ttttttaatt atttccacaa actcattact ctttttatat
     2941 ggctgcatca ttgataaatc agcaaatgta tccagtgctg tctggtaatc tttcagggca
     3001 aaacatatct gtccaaatag ataattaaat tctttatggt caggtttttc ctttaacgcc
     3061 tggagtattg cctttttagc ttcttcataa tgcttattct tcattaacaa tttagccttt
     3121 agataattat acgatgccga aagaggaact gttgatattg aatttaagat tgacagggct
     3181 tcattataat ttttcttttt atacagagta atggcttttt taagataatt ttcaacagct
     3241 acaggctcac ctatctcaat aacacaaagg gtcacatcat cacgaatgac accatcttcc
     3301 gattgaatga aattttcaag atctttaatg attatatcta tttgatcctg aggagcaaat
     3361 tcccgcgatg caacaagaat attggtaaaa cgttcattac catataaatg cccgctcttt
     3421 gcccttgttt cagttatgcc atcagaatat aatataatac ggtctttggt gtttagctca
     3481 aaaaggcatt cagggtaatt aatatgttca tcagcgccaa taaaaagtga atcggttgtt
     3541 aatggatata ctgtatcgtt taatgtgata atataaatat cagtatgccc acagttggta
     3601 tataacaatt ccatattgac aaaatcaata atacccaaaa acagtgtaaa gtaaaaacca
     3661 ctttcctgca atatcggggt aagctttttg tttaactgag tacagattgc agaagttgat
     3721 ctgtataatc gtgataaatt ttcaatttca gttttgatca ttgatgtaag taacgctgat
     3781 ggaatcccat gccctgaaac atcaacaata cctatcatat aatgtgtatc atcaagctgc
     3841 tccattaaga agaaatcccc acttacttcc atatacggcc tgaaatatac tgaagcatac
     3901 aactgattat ttgctggaag aatagaaggc aaaagcgttt tttgaactgt ctttgctaat
     3961 ttaagttcaa attccatctg ctcatttttt ttctgaagct gttcagttct ttgcattacc
     4021 agagcttcca gattattctt taagttgctc aaactgtgat acataagaga attggcgatt
     4081 gcaattgatg aagcagattt aacattttgc aggaattcaa tctcgtcatt ggataatgct
     4141 tttaaattag cgcgttcaga taaagcaata ataccaataa actcattatg ttccaggggg
     4201 ataatatatt ctgcagataa tttttcaaac cagggcaata aaatattctt tacatgttca
     4261 tactgcggct tattttcaat cagttttttc tcaatcaccc atggattttt taataaataa
     4321 tcctgcacct gttcatctat tactataaca tctccattga ggttatataa ttcattttcc
     4381 ttatcttcag ctttaataaa aatttccaca tttttaatga gcaacgtgcg ttttaatata
     4441 tacgatagtt catttaacaa cagatgtaaa tcttttaaca atgcaatatc attaataaac
     4501 tgtgattcaa cttttttcag attatatttt cttcggttaa aaacctggtc tatgagtggc
     4561 tgaactttta taaaatacag atagttaact gcaaaccaga tagttaataa aaaaaacagc
     4621 aatgatttat caatagattt aaaaaaagga tacaatatat tgaaaataat cacattgggt
     4681 acaacaatca ctgatgaaat gcataaccat ataaatgtta catgcaacac actctgtatg
     4741 tccattaagc gataacgcag aataccatac cccagaaaaa taagtgggat aaacattaaa
     4801 ttgctcaacg gataaaaatt atacccaagc atggcaggaa cattacatgc cgtaaaaatg
     4861 ccaattaaat tcaatgccag tatgatgtat tttattttta aacgaactaa ctcatttttt
     4921 tcctgtttta attttaagat actgataaca atcccataaa taagaacaga aaatgcatat
     4981 acaccaaaaa tctgaaacac tataccacct ttagcaatgt gcccccatgg gtatgttaaa
     5041 agatctgtga tgtaatatgg tgtatggact aacaatgcaa aaattgcact tacaataaat
     5101 gttattatct caagatatgt tcgttttatg ttcaatgcag tgtgataaaa taacatacct
     5161 ataaaaggta taaaaacata aagggtatgg atggaccttt ccattttcaa tatagtgttg
     5221 acatctttaa ttataaaatg gcctaaaaaa ttgggagtaa gcagcgcata ccataaacaa
     5281 aagagtgcaa acaatatatt ttcttttttt agtttacctt tcaatattgc tatgccggca
     5341 ataactatgc tggtgagcaa ggttatcaat ggtggcaaca taaaaatgtt tagcgatgca
     5401 ataatatcgt gcatatacaa cctctacaac cgcaaaaata tttatagcac tgtattttta
     5461 aataatgtta tagtcaatag tatttttttc aggttaaggc ttttcgtcct ttaggaattt
     5521 ttcagtcaac aggcttcatt tctttaatta tactatcgac aatatccgga atttcataaa
     5581 aggagtgtgc aatccaacga caatgagcta tctcttcatc attatgacca aaagcacagg
     5641 caataaacgg cacattgttt ttgtgcgctg ccaccatatc atttgctctg tcaccagcca
     5701 taattacaat gtcacagtga ggcaacgatt ccttataata tgccagtaat tgtgttttat
     5761 cgtgcaaatg tttgtcttcc agtgctgtta ttgtaaaaaa gtattgtctg agtttataag
     5821 catcaatcac agcttcaaca tattcttttc ttccatttga tgctgttact aacccatatc
     5881 ctctattgta cagcactttt actgtttcaa caacaccagg tagtagcgtt cccttttgct
     5941 gtagaacaag ttcagcaagt tttacggtac aataatcatt aagcgtgtgc gatagttcct
     6001 gtgaaagagc tgggaagagt aaagcaaata tctcatcaac aggaatccca acaagcttca
     6061 tgatttcatc agtggatggt ttgcgcaaat taagctcggg atgttgcact aaaaatgtat
     6121 caataccctg ttcaaatgcc atcccaacaa caggtgagca atcgtataat gttccatcga
     6181 tatcaaatgc aatgtatatc atggtggaaa tattttttaa ttacaggttt tggcaacaaa
     6241 attttgtttc aataatttat tttcttgact catttgtggt ataattatac ttcacaattg
     6301 caaattataa ctatgctgct ggaacaaccg tgaatctttc tgtaacaatt ggcaatctac
     6361 aactaaaaaa ccccataacc gttgcatcag gcactgcagg ttatggcgaa gaactttcgc
     6421 aatttgttga tattggtagt ttaggtgcaa tctttacaaa agggttatcc ttaaaaccgc
     6481 gtgcaggtaa tcctggtcca cgtatagctg aaacaccttc agggatgctt aattccatag
     6541 gtcttgaaaa tgttggactt gaagcattct tatcgcacaa gctgccgtat ttacaaaaag
     6601 ccaatgctac tgtaatacct aacattgcag ggcactcaat tgaagaaaat gttgaactgg
     6661 caaaaacact ttcaaacacc aatggcatag cagctattga actcaatgtg tcatgcccta
     6721 atgtaaaaga agggggcatt gcctttggac aggacgttgc catattttca agattactgg
     6781 aaaaagttcg cagtgtaacc cgtggtgttt taattgttaa gctttcacca aatgttactg
     6841 atattacaca atttgctaaa gccgctaaag aaattggcgc tgatgcagta tcagcagtaa
     6901 atacatttgt aggaatgaag attgatatag caacaggaaa gccatacttt gcaaatacat
     6961 ttgccggttt aagtggccct gcaattaaac caatagcatt acgcatggta tatcagattg
     7021 tccagaacgt tgatataccg gttattggca ttggcggcat ctttagtttt gaagatgtgc
     7081 tggaattttt aatggctggt gcgcaggcca tacagatagg tactggaaac cttattcatc
     7141 ctgatatatc cgctgttatt ttgcaacagc ttgaaaatta catgaaaatg cattcaattg
     7201 atactgtaaa agacttgatt ggcattgcac aaaaaaggag taaccaatga agtactggaa
     7261 caaacgtctg cagaagatga ccgaatacat ccccggtgag cagcccgata atctgaatga
     7321 attcattaaa ctcaacacta atgaaaatcc gtttccgcca tcatcggctg tactggatgc
     7381 attgcagaaa gcatgcaatg gggatctgcg ccgctatccc aatcccaatg ctcagatggt
     7441 acgggaactg tttgcaaaaa agaattcatt agcagctgat aatgtttttg ttgccaatgg
     7501 ttctgatgaa atatttacat tgctgttcag aggatttatt gaacctgatg gcattgcggc
     7561 gttcccctat ccttcgtatg cactctatta taccatggct gaagcaaacg ggattgcgta
     7621 tgataaaatt acccttgatg ataactttga tataccattt gatgaattct taaaaaagaa
     7681 atataatctg gttattatag ccaatcccaa taaccctacc ggcaaagggg ttcccattga
     7741 ccagataaaa aagttttgca gtaaattcaa agggttgctt gttgttgatg aggcgtatgt
     7801 tgacttttac aatgaaacag ctctgccact agtcaaagaa tttgataata ttgtgataac
     7861 ccgttcattt tcaaaatcat attccctggc aggtttacgg gtaggcctgg cgatagctca
     7921 taaagaaata atccacggtt ttattaaact aaaagactca tataacgttg acagaattgc
     7981 gcaggcagga gcatatgttg cgctgcttga tgataaaggt tttaaatata atatttcgat
     8041 ggtgcgtaac aataaagaat atctggaaga gtcactggaa gcattgggct ttataaatgt
     8101 tccttccaga gctaactttg tgtttacaaa acatcctgat atcgacgccc gtactctgta
     8161 cgaaaaacta aaagaacaaa agatactggt gcgctacttt gcaggcccca tccagtcaga
     8221 gtatattcgt attagcgtgg ggacaatgat ggaaattaaa aaacttatca aggtgctttc
     8281 tgaaataata tcaggttaat aattttacca tccagtctgg tggtggtgct gcgcgcaata
     8341 agctcgggat atattatttt gttttgcata tattgttcaa tgtccttttt ttctatccat
     8401 agtataatcc cctcagaacc ttcaaaaaac cagtaaaagc ttataacaag tccctggata
     8461 ttctcgtcct gtaatatctg atgcagaatt tttgattttg tcaatactgg atttaagttt
     8521 ttaaagataa tattccatgc acgcagtgga taagaatcgg caccagtaat ggtaccaccc
     8581 acatcacaat caaaacctaa atataattta tttttttctt ttgttttatt atcatagtaa
     8641 aaaccaaatg tcctgattct gggggctaat ggctcaatct tttttatctc caaacccaga
     8701 tcattgagtt tggtgttata cattgcattt aattttatac cttcctgtgt ggtatactga
     8761 aattccggta cggcataatc attactgact gctgactgtt gcgttgagct atcacctact
     8821 gtttgttgta catttttttc attagcagtg gcacaggcgc taacaatgaa caatgataaa
     8881 acaactaaga ttgacaatct ttgcatatac tccctcatta taagccacag tgttatagtt
     8941 tttttaatgc ttctacaggt attgaaagca ttgctgttgt gttattaatt gaaatgactg
     9001 ttaccgcttt ctcagggtca atgcctaaat tgtacaaata gctatcaatc ttggcatacc
     9061 ccaggccaaa accagaatca cttgtatcaa tattatttat aaaaaactca tattctcttc
     9121 cttcatcctt atatcggcgg tattcttcgc gctttgcata aatcctttgc aaatcatggg
     9181 aaaggatggg ggctgtgttt gtaatttcta tataaatata attatcacgc aattcaatct
     9241 ttaaaattat tctgatatca tattgtttca gcttttgttg caattctaca aacagtttta
     9301 atttttcaat tgattgcaaa tcatcactgg taagcgaacg attttcacta aaggctttat
     9361 tcttgataag taaaaataat ccctcctgat ttaaaacttc tctaaccttt tgtgcaatat
     9421 tttctttttt taatacaatt gaagccagcg tatcaaactg tattttgtct ttaagaatat
     9481 ctttgatata ttcctgtacc tgtttgcccg tcttatcttt gtactgcaac tggaattgcc
     9541 gttggagggc ttctacaatt ataaggtgct tatagtttgc ttttactgca tttttaataa
     9601 gttcatctac acatgcacca atatcatcca gatgttgttc acaatttgca gccttgaata
     9661 ttgtaagtcc tgcattgtaa atctggtcac gtgctctccg tgtataatgc ctgattatcc
     9721 ctgtaatgcg aagtgagttc ataactatta tttatacaga aactccatga acttttgcaa
     9781 agcctcaggc aatgtcccat catcacgctg gtatttttta aattttcgtt cataatttcc
     9841 cacttcacga ttaaaaacat tcctgaatct gttggctatt tctgtatatg caggcatatt
     9901 ttccagcctg tctctcaatt cttttttgaa tggtatgtga cgataaaaca tatctcttac
     9961 aacattattt aacttcataa tgccatcttt ttcatagaga acccactgta tatagtcatc
    10021 tgcaaatctg tcgcggttat tacgtatact cttgaaacgt tcaacaacct tctgtttggc
    10081 ttcaagtgat agcttggggt tctttttaaa tgtattgata taatcaaagt aagccccggt
    10141 caacccacct tctattgggt cagcccacat tcctcccata atggtacggt taagctccca
    10201 ccggaatgtg gcaaacgtat gtgccattgt gggcatcatg tcccccataa acaatatagg
    10261 aaccactatc ctgccccgtg ttcttctgtt tgtcccatcc agttcctgcc atagcattgt
    10321 ttttgtgccg aaggatggca ataatataaa atacggaaca acttcttctt ttataagctc
    10381 cttggtgttt ccaatcatta ctgctgtttc acgataaaaa acagaataat caacatccct
    10441 caatgcatta acaatagatt ccagtttctt tttagataca aagtaatgtg atatgctgcc
    10501 acgcatattc atcgatgtca atattgggaa tgatgttgct gtagaaccag aacaaaccga
    10561 agaagcagtt gcaatacggt gatttatttc ataaatgact ttctgcacat tatctgattc
    10621 atcttttgca ttactgcctt tgatgtgttt ttgttcttcc cgcagatgag cttcatagga
    10681 taaacccatt tcattaatac tgggattttc ttccccattg aatatttttg ttaaaaattc
    10741 atcttcatat aatattggaa tatctgtttg ctctgtcctc cgcattgaga tttcatgtaa
    10801 ctcagctagc tgttcctgct caatcatagt ttcatcaaaa aaaccaaacc ggagcattag
    10861 cttaaccggt tgaggcacag cagattctga ttgactgcgt acaaaaacct ttgcataaat
    10921 atcataataa aaccgtgcta tctgccttcg tatcttacgc gcatcaattt ctgatgaaag
    10981 agggtccttc atttttttaa attctgataa taacttcaaa agatttttct ggttttcttt
    11041 ttctatcaaa ccaaactcaa aaatttgata tatagaattt ttataaagct ggctcaaatt
    11101 ctgagggatt cgtgaaggcc ttggtgcttc ttctgcggtt tttggctgtt ctttaatagc
    11161 ctgtacaaaa ttactgagct ttttgaaact atcatatttt tccgaaagca actcnnnnnn
    11221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacttca ccagcaacaa gagaaaagtt
    11341 gtcattcact ctgcaaagat tctgatatag caatgtatat ttcttaacat catttaatga
    11401 taattctaac ctttttaaca catgagtaag caataaagca gcaaaagaag ggtcatcaac
    11461 aactatctgg cggaatcccc cttcccgtaa cggatatttt ttgactacac aatcctctat
    11521 ggcacgtata gtctttttat atggttcagt aaataaaagg cttaagccag ctataacact
    11581 tttttcatta attattccaa cacgtttgct tttctcaaca ataatcttgc tatcaagccc
    11641 ttcatattct gaaggtgcag acaatatttc aactgatccg ctctgtaaat aataaataaa
    11701 cttaggttct tcaccctgca aaattaaaat ctcgcccttt ctgatttttt cagtgccatt
    11761 attttccata atcttttttt aacacaaaag gtatttttta ctatttaatt aatttttgca
    11821 ttagtgtaga gtactattgc aaaaagagca ataacaattt tacttaaaac caattttttt
    11881 tgggtatgtg ctcgcaacat acaaagaata gttataataa caattttact tcataccaat
    11941 tttttttaaa ttctgtatat attcttccca ttcaacaggc aacaatgact ttttctttat
    12001 attgcattcc ttgcaacagg ggacaatatt tattttttct gaatatccac ctcgtgaaag
    12061 tggtattacg tgatccatcg tcagttctga tggtttaaat ttcctgccac aatagtggca
    12121 tataccaggt gccacccttt ttttccacca cgatgagtgc ctgagttccc gggctttttg
    12181 cttttcacgc tttacatgtg tttccagatc cgaaaatcca gaatccacaa aatgaagttc
    12241 ttttggttta aatttttttg atctgtcacc cataccgttt actcaaaagc atcaccgtgc
    12301 tgcaatcggt agccattaat aaaagcgctt gcatccattg gctttttggt ctcgggttgt
    12361 aatgacatca cttcaatagt accatcaaaa gtgcacacaa atagacgctt tgcctgcacc
    12421 accaccgtac ccgggcgggc agtggaatta ttacaggagc tatcgctaag gagggttttc
    12481 catattttta taattttacc cctgaatgtt gtccatgcta ctggttttgg attaaatgcc
    12541 cgcaccatgt tatgaatgtg tatcgatggt atatcccagt ttattcgcgc agtttcactg
    12601 gtaatttttc cacaataggt tgcctgcgaa tgatcctgtg gcactggtgt aatagtgcca
    12661 tcatgcaaac ctgcaatagc tcttaccagc atatcagcac caaggggcag tattttttca
    12721 tatacatcct gtgcggtttc attgggggcg atagttaccg ttgattgaag aacaatgtca
    12781 cctgcatcaa gctcttcatt aatacgttgc acggtaaccc cggtctgctg ttcaccgtta
    12841 tacagcgccc attcaaccgg tgctgcacca cgatactttg gcaaaagtga tggatgcaga
    12901 ttaatagttt gcagtggtgg gattgaaaag attgaacgag gaataagttt gccataggct
    12961 acaacgacaa agatatcaca attgaattgt ctcaacgttt ctgcaagctg ttcatccttt
    13021 aaggttgttg gttgaaaaca cacaattcca tattcaaggg ctttttcttt tacaggggta
    13081 ggcaacagat gtttgcctcg accttttggt ttatcaggag atgtaacaac aaaagctatc
    13141 tcataatgtt ttataagatg ttctaaacag taagaagcaa tttcaggagt tccaaaaaag
    13201 cctattttca tcatgccctg ttaagttttt ttatcttttt caactgtgca ttgagctctt
    13261 ttcgtttgta atcctctaag taatctataa acaatttgcc atgtaaatgg tctatttcat
    13321 gctgtaatac ccgcgccaga agtcctgatg cttcaaattt tactggtttt tcattgatgg
    13381 ctatgccttc aacctgaatt gaaacagggc gtataatatc agctgtaata ccaggaagtg
    13441 acaggcatcc ttcctcataa ccatccgttt catcagaaaa tgctgtgatc tttggattta
    13501 taagtgccat catagggcct ttatttgacc gtatatctat aactaaaagc tgtagcggaa
    13561 catcaatttg cggagccgcc agtcctatcc cttgctcctt atgcataatt gcaaacatct
    13621 catcagttaa attgcgtatt tcctgagtaa tctgagttat ttcctgagca actgaccgca
    13681 atgttttatt accataataa actaatctat gctgtgtatc agttaccata attatctcat
    13741 aaaaataaat tatactaatt gctataatat accacaatta ttttttactg tcaatataag
    13801 gtttttaaaa aaactactat aaaaagatat ttacatttac ttattgacaa tctcatatat
    13861 attgtttaca attttacctg aatgagtttg aatgagttgt acttcatttt tttatttgca
    13921 tattggtcat tcatccaatt tactttgtgt aaattgtaaa tatattggaa tttaaattag
    13981 tatagtatta tttcaacagg aggccattat gccattcaaa gaatatgata actacgatgc
    14041 cacaggatta gctgaactca tcaaaaaaaa gaaagtatca attaaagaag ttattatagc
    14101 agccattgaa cggatagaaa aatacaatcc tacccttaat gctgtcatca ccccaatgta
    14161 tgataatatt gatagaataa taaaagaact ccctaaaggc tatttttatg gcgtaccttt
    14221 tcttattaag gatttactaa cagcttataa aggggtccca atggcaagcg gttgcagagc
    14281 atacaaaaac tacatacccg attttgacag tgaggttgcc aaacgctata aaaatgccgg
    14341 tcttgtcatt ttaggcaaaa ccaatactcc ggaatttgga ctggttgcat atactgaacc
    14401 tgaaattttt ggcccaacgc gaaatccctg gaatactgct ctgacacctg gcggttcaag
    14461 tggtggttct gctgctgccg ttgcaagcgg gatggtacct tttgcaggtg gtggcgatgg
    14521 tggcggatca atcaggatac cctctgccta ctgcggtcta tttggactta aaccgtcacg
    14581 agggcgtgta cctacaggtc cggaatacgg tgaaatttgg cagggggctg ctgtggaaca
    14641 tgttttaaca cgctcagtgc gtgacagtgc tgccatgctt gatattttgc agggctctga
    14701 ccccggtgct ccgtatatca ttgaaccacc aaaaatgcca tatagcaaag aaatacagaa
    14761 agcacctggt aaacttaaaa tagccctatc aacagattca ccaattggtt cacctgttca
    14821 tcctgaatgc aaaaatgctg ccctggaagc agcaaaattg tgcgaaaaat taggtcatta
    14881 tgttgatatt gataaaccta atattgacgg cctggcactg gcaaaaagct accttatgct
    14941 atactttggt gaggttgctg ctgaaattag gaccattggc atcaaattag gacgaaaacc
    15001 aaaacgcagc gatgtagaag taaccacctg gacattaggc cttttaggca ggacatacac
    15061 tgccggagat tttgttatgg cacttcgcga atggaataaa gcggcacgag caatggggca
    15121 attctttaca aagtatgata tatacctcac accaacaact gcccaacctc ctgcacccat
    15181 tggttcactt gctccaaaac caattgaatt atttggttca agaattgtaa atatttttgg
    15241 tcttggaaaa ctagccatag ccagcggtgt tgttgataaa ttagcagttc agaaccttgc
    15301 acaggtaccg ttcacacagt tggcaaatgt aaccggtatt cccgccatgt cagtacccct
    15361 gcactggggt ccagatggct taccttatgg agtacagttt atggcagcgt ttggccagga
    15421 agcattgtta ttaagactgg ctgcacaact ggaaaaggca aaaccgtggt ttaataaacg
    15481 gcaagaacta tatcataggg ggtaatatca tcatgaaaaa ggttttgaat gcacttattg
    15541 aaatactatt cttcttctac cggccaatat tttaccttct ggtcattata gatacaacta
    15601 tacttggtat tcttaccata acactttcat tttttgatcc tacaggtaat accattcact
    15661 acattggagt attctggtca cgccttaatc ttttcctttc cggagtacgt gtacgtgtac
    15721 ataaccgaga gtacataaaa aaagatcagc cctacattgt tatgatgaat catcaaagcc
    15781 attacgatgt gtgggcagcc attggctata tcccgttaca actcagatgg gtcatgaaaa
    15841 tggagttgcg aaaaattcct atctttggat taggatgtga acgcatgggt catatttaca
    15901 ttgaccgtgg cgattcacat caggcacgcg aaagccttaa agctgccggt gaaaagattc
    15961 gtaacggtgc atcagtggtt tttttcccgg aaggaacacg cagccctgat ggcaaacttt
    16021 tgccttttaa aaaaggcggc tttgttatag ccattgaatc tcgggtgccc attcttccta
    16081 tcactggatt aggatctcga gcaatactgc ccaaaggaag tatggctgtt cgtccgggaa
    16141 ctatcgacct cattattcat ccaccaattg aagttaccgg ttatacccag gaaaacaaag
    16201 aagaactcat ggaaaaagtc agagctgtaa ttcagagcaa gcttgtatta taaaaaattg
    16261 tgttgtaaaa tttgttttgt ttttttattg tgcgatagtt cattataata accccttgcg
    16321 ttataagggg ttattttttc aaatcacatg tgaaaaaagg acggtataca gcagtgaact
    16381 tcagagaacg attatataag ccctacccac ttgtcatgga tggtgctatg ggcacaatga
    16441 ttttcaatgc attgcctggt tataagggaa atcttgagat tctttctata gaacgtcctg
    16501 atgtaatagc atcaatacac aaacagtata ttgatgcagg tgctgatatt attgaaacca
    16561 acacgtttgg cggaaatgca ttgaaactcc atgaagctgg tttagacagt cgatgcgaag
    16621 aaattaataa agctgctgct atgttagcaa aggatgtggc acgtggtcag gcgtttgtag
    16681 ctggttcggt tggtcctact ggaacgttag ttgaaccaat gggaattaca tccgcagaaa
    16741 ctgtatataa cgcatttaaa cgccagatca gtggccttgt agctggtggt gttgattgta
    16801 tcgctattga aacaatgact gatttacagg aagcacggct tgcacttctg gcagcaaaag
    16861 atgttaccga tattcctgtt atctgcagca tgacgtttga acaaaacagt aaaacagtaa
    16921 ccggaacaga tatagtcact gcacttgcca cactttcaca gatgggtgcg gatattgttg
    16981 gagctaattg cagcatgggt ccagatggcc tggttgcact tttcaaagga actatcgacc
    17041 agcttaatga attgggcgta cctctttcag tatgggccaa tgcagggctt cctgagctta
    17101 ttgacggaaa agctgtgtat agcctttcac caaagcgttt tgcatcactg tcacttgaat
    17161 ttgcacagat gggctttaca atcattggcg gttgttgtgg caccacgcct gaacatatca
    17221 gaatgctagc tactgcagta aaacatattc ttatcaatac aaaaaagact aaaacattcc
    17281 actacattac aagccgttat acacatatta acgttgtgaa tcatacggga ctgcttatca
    17341 ttggagagcg gttaaaccca actgcacgca aacaatttgc tgctgaactt aaagaaaaca
    17401 catttacctt cctgcgtaat gaatccaaaa aacagcagga tgaaggagca cattgcctgg
    17461 atattaatgt tggtgttccg ggcattgatg aagttgccat gatgaaaaaa gcagtaaacg
    17521 tgttaagcaa tacagtaact atccccctca tgattgattc ggataatcct tttgtaactc
    17581 aggctgcatt gtggcaatat ccaggaatac caattattaa ctctatcagt gcaaagaaac
    17641 aaagctttga gactatgctt ccacttttaa agaaatttgg tagctttgtg gtagcaatgt
    17701 gcctggatga aagcggcatt cataaagaag caagcaagcg tattgccatt ggcgaaacgc
    17761 tcattgattt actgcaacat caaggcattg ccctgtcacg catttttatt gacccgctta
    17821 ttttagccga aagcgcagaa agcggcgcag cacaggaaac actgaaagtt attgaacatt
    17881 ttgcaaaaaa gggaatcaaa acaagcattg ggttaagcaa tatatcgttt ggcttgccac
    17941 aacgcaaatt tatcaataac gtatttttga agcttgcttt acaaaggggc ctctatgctg
    18001 caatcgttaa tccaggcgct atcgacacca ctactgcaaa accttactct accgaagaac
    18061 tgcttgcact ggattttctt gaaggtagag ataagaatgc ttcacattat attgcacatt
    18121 ttggaacagg tcagcagcaa atgcaacaaa aggaaaaagc tatatctata cttgatagaa
    18181 tatttgaact tgttgttgca ggcaataccg atgatattga accagccgta aaagaagcat
    18241 tgagccagca cactcctgaa acaattatga atgatgcact cattgccgcc ttagaaaagg
    18301 ttggccaact ctacagcaat ggcgaatatt tccttccaca gatgattgcc tcagctgata
    18361 ccatgaaaaa aggttttaat cttttaaaac cactgatgct gcaaaagaag gaatcagtgc
    18421 tgggaaagat agttatctgt accgttaaag gtgatataca cgacatcggc aaaaatattg
    18481 ttgccatgat gcttgaaaat cacggctttg atgtgattga cttaggcaaa gatgtgccaa
    18541 atgaaaaggt tattgaagca atacaacagc ataaaccaga cattgtgtgt ttaagctcgc
    18601 ttttaacaac taccatgcct aatatgggta ctattgcaca tgaaattaaa aatcagaagc
    18661 tgctatgtaa actgatgatt gggggtgcgg ttgtgactaa agaatatgct gatagcattg
    18721 gtgcttttta tggaaaagac gctgttgaag ctgttgctgt ggcaaaaggt attgtaaaaa
    18781 aatctcaata actttttttt ataaatttag ttgacataaa gattgtatat acaagaattc
    18841 gcttatgtta ttatagtgga ttgtagtaca aatccgcttc ttttattata tatttttaca
    18901 ggtttttaca ttacaacaaa gattaaggag cacgtctgtg aaagggacac gttcttttct
    18961 actctttttc ttaccttttg ttatatttgc atacatctca tttatagtat ttcagcaggc
    19021 ctgtgattat gctatgaatg tacaggcaaa gaaacttctt ccattcaatc atcgcacaca
    19081 tactaccaca tatggtgctt cagactgtga aatatgccac agttatgata gcaatggaag
    19141 attcaatggt atccctacgg tggcagtttg taaacagtgt catgatccaa acaaagcaaa
    19201 tacccaagtg ttttttgctg gttttggtga taatgacaaa ccatgggaat cttttgccca
    19261 gcagcccgac ctggtatatt tcagccacat tgctgtgatg aaaaatgaaa agaaagcaca
    19321 gtgtatctca tgccatggtg ataagggcaa ttctactaca actgctaaaa ttaagggtaa
    19381 aatggctatg ggtacatgta tggattgcca tacagcttta aaaatcagca atgcatgtac
    19441 agtatgtcat gattaaattc ttattattga atcgaaattc aagcaaggag ttattacatc
    19501 catgaaactc gaacgaaaag atttcttaac aatgataggt ggagcagtag taggtactgg
    19561 tgttggtaca ctgatttcac ccggtccatg gatgggtctt caatggctgg ttgaatggac
    19621 ccaggaccag tatcgccctc ctaaaggtga agaaaaatat ttagcaagta tttgtgacca
    19681 gtgccccaat aaatgtacag tacaggtaag aatgattggg gatagagctg ttaaaattga
    19741 aacatccaat cagggttgtg ctgcaggaca gctccttttg caggtccttt accaccctga
    19801 acgagtaaaa accccattaa aacgaaccgg taaaaaagga aaaattgatt ttgagccggt
    19861 ttcatgggaa aatgcaatag cagatatctc caaaaaaata aatgatgcca ttaataacaa
    19921 aaaacaggat caaattgtag caataaatcc tgctttccca gatgcaacag caatgttaaa
    19981 cgaaagaata gcactttttg ctggtagtcc tcatgtatac caggaatcag gatactgggc
    20041 tctctccagt gcctgttcac tggcaaccca gaaaactgaa ggagctatcg actatgattt
    20101 tgaaaatgct gattgcgttg ttagctttgg tgcacgtctt tttgaaggat ggggaaatcc
    20161 aggcaggatg cataatgtat tgaatatctg gaaacaaaaa ggtgtcaaat ttatacaggt
    20221 ggattccatt gcaacacgta cagcttcatt agcagaccag tgggttgcaa taaaacctgg
    20281 cagtgaacca atttttgcta tgggcgttgc acattatctg cttaaatatg gcaggcgctc
    20341 aggtgcaaca aattttggca gatttacaga gctattaatc aataaattca ctccagaaaa
    20401 ggtttctgag ctaactggtg catctctcga aacaattgaa tcagtagcaa aagcattact
    20461 gcaagcacga aggcctgtag cagttgctgg caaaggcgga aaagttgtat cttcttcagt
    20521 tgccgaattt gcaataatac agacaatcaa cagcatgatt ggcagtcttg gtaacgctgt
    20581 taagcttgtt accttcgcaa ctcctgcaaa gccagtaata gcaaagaagt ccaagggact
    20641 tgatgagtat atagcaagcg atatgacctg tgacatagtg ctgataaaca atgctaatcc
    20701 cgtttattca agcgtatatg gtgagcgcct atctgaaaag cttaaaaaag cttcaaccgt
    20761 tgtgctgttt acttctttac ttaatgattc tgcacaatat gctgattata ttttcccaac
    20821 acttacttcg cttgaaattc cttttacagg tcaggctgta tcagctaagt ttgaagcaaa
    20881 acatgctggc gattcattgc ttgcaatagc aaaaaatata cccaatatag ctgctcaatt
    20941 cccatggaat agttatttag atgtggtaaa agctgtaaat ttccaggcta aaaatgtagc
    21001 aaatctggat atccctgttg atatcattac tgcaaagctg gaagaattag aaaaaatgct
    21061 gcaagcttca ggctcatatt ccatatcgat ggtacctttt gaaataccag tagttggcga
    21121 gggaaatagt cttgcacttc cctatgtatt aaaagccctc ccaaatacaa ttttaaaagg
    21181 cgatacaagc tgggtatgga tgaatccagt tacagcatca aaagaaggaa tttccgaagg
    21241 ctccaaaatc gatattgaat cttcccgcgg tgaacttgac acagtgagag tacatttaac
    21301 caaacgcgtt gcgtatgata ctgttgcaat gcctatcggt tttggccatg aggaattaac
    21361 cgaatatggc agcgaaaaag gtgttaaccc aaaacccata atgtcagatg cattggaccc
    21421 tctcagtggt tatgctgact ggtggttaac acgagtaaaa ttgagttaag aggtgattaa
    21481 ttatggccaa gatgacaaga agatggggaa tggttataga tttagataaa tgtactggat
    21541 gtggtgcatg caatattgct tgtgcacagg aaaataatct cccaatattt aaagatgatt
    21601 ctgatgtgcc caaaagggtc acgttccttg accttattga ggtaaccaat gataaagatc
    21661 ccaaagctca gtatcctgat gtaaaagtgg catatatacc aaaaatgtgt cagcagtgtt
    21721 cagggaatga cccaaataat ccacatccac catgtgtttc agtatgtccg gttgttgcaa
    21781 ctgatgtagg tgaagatggt gttgtaagcc agatatggtc acgatgtata ggctgcagat
    21841 attgtcaggt agcctgtcca tacgaagcac gtgtattcca ctggtggaaa ccccgttatg
    21901 aagggtcttt caaagaatct cttaatcctg atgtttctat agcatctcgg ggtactatgg
    21961 taaaatgtac cttctgttca catatctgga agcgtgaacg tgataaagca atagcacagg
    22021 gtataaccga tataaacgca gtaacgtaca ctccagcctg tgcaagtgca tgcccaacta
    22081 atgccatact atttggcgat ctcaatgatc cttcttcgcc agtatatgaa cctaaaatac
    22141 agaaggatcc cagggctgta cgtctggtac attccataga tgaattacgt acaaaagatc
    22201 cgtctgaata tgaaaaacgg actaaaatca aagaatatcc aaatccaaaa gtatattatt
    22261 taaccagcca gcaatggctt cgtgataaaa tatctggatt taaaaactcc taaaaaaaag
    22321 tataaggaga gtataccgtg agcaaagtaa tagattttct aaaaaatgaa tggaatagtt
    22381 accccaggaa tatacagata tctctgtatg tcctctttgc actggtagca ctgtttgtaa
    22441 tttttgctat tcagatcctg ttttggggat taggttacac cgatatgaat aaccgctttg
    22501 ccatggggat atggataatc ggtgatttag gttttgttgc gctgggcggt ggagccttct
    22561 ttacaggttt tatgctgtat atattcaggg ttgataagct tcagccttta ataaattcca
    22621 ctgtccttat tggttttatg tgctacctgt ttacatttat attgctggta tttgatattg
    22681 gccaaccact gagggcctgg tttggatatg cataccccaa ctggggacca cataccatgc
    22741 cacaatcaat gcttacagaa gtagtctggt gtttgacatt ttactttatt gttctttgca
    22801 tagagctttt ccccattata ctcagcaata aatatttaga tagatatcca gcagtacatt
    22861 attttgcaca ttacttgcat aagttaatgt ggatttttgc tgccattgga acattcctct
    22921 catttttcca tcagggatca cttggtggtg gaatgtgggg tgtattatat gccaagccat
    22981 actggttcag gcatcattat ttctatttgg cgatagtagc tgcgatagcc ggtggtccct
    23041 gccttatgat gcttgctcca aagatagcaa gctgggttgc caaaaaagag cttgtaccca
    23101 aagatgccta tatgacatta gcacagatag caggggtaac atttattgca tactttttat
    23161 tcagagtgta cgatctttac acacttgcaa aacactttgc accactattt gaccgcaatc
    23221 tggttgatat gatgggtggt tattatggct ggtgggtagt tggattggaa ttgcttgctt
    23281 cactggtagc aattgttatt cttaatgtaa aaaaattccg tgaaaacgaa cagtacttca
    23341 ttgctggttt aatcagtggc gtgcttgcta tagttatgct gaaagttgat gtgttactcc
    23401 atggattctc aataccaaac ttcccatggc aggatttctt tggatataat ccaactattc
    23461 aggaatggtt tatattctta ggttctcttg catgcatgac tcttatttat atgtggtttg
    23521 taaaatacat accgctattt cctgaaaaac accatgaaga agctcactaa agatttgatg
    23581 atataaaaaa aataaaaacc ccacgatttg tggggttttt atttgtataa ttcacatttg
    23641 aaatgccagt tattcattaa ttgctatgtt attttgtaat ttaaatgctt taactgtatt
    23701 ttttaatagc attgctattg tcattggccc caccccgcca ggaacgggag ttatagcatg
    23761 cgctacttca gaaacctcat caaacttcac atcacctact aattttgttt tacctggatt
    23821 ttcagggtca tttattctgt ttatgccaac atcaataaca actgcccctt ttttaaccat
    23881 atcttttgtt atcatattag ccttaccaac agcaacgacc agaatatctg cctgcctggt
    23941 ataatatcct atatcctttg ttgctgtatg acaaactgtc acaatacaat ttgctctgtt
    24001 atttttctgt accattaaat tggccagtgg tttgccaaca atattactgc ggccaacgat
    24061 cactatatgt tttcctttaa tatctggcat tgtccgcaca atgagttcct ggcatccata
    24121 tggtgtacac gggataaagc agtcttcacc aataacaagc tttccagtat ttactggatg
    24181 aaatccatca acatctttca caggatcaat tgcatttatg atttcatttt cattaatatg
    24241 tgagggtaat ggtaactgca caagaatgcc attaacattg ttatcattgt tgaactggct
    24301 aatgagagtt agcaactcct gctgggtagt atgttcatcc agcagtatct gccaagaatt
    24361 aattcctact tcttcagagt ttttcttttt attgcgtaca tatacttcag atgcaggatt
    24421 attacccacc agaatgacag ctaatgctgg ttttttccca tacttttgtt caagcttttc
    24481 aacttcttgt ttaacttctt ctttgatttc attggctata gccttgccat caatgattat
    24541 tgccatgttt acctcatgtt gtattataaa ttatacatct attactataa acgattccca
    24601 ctcgttatcg tttatttttt cacattttac cttatgcatt gttactgctt taggatcaat
    24661 atttatatca tacccatttg ttatataatc accagtacaa cgagcttcac aactaacagc
    24721 atcattgcta tatataattt gtatagtttc aggatatact agcaggcctc tggtatcttt
    24781 aataaatagc aactcgttta aaaatttgac gagcaaaaga tcaagataat tatgctgaag
    24841 caaaacatga catttctcta ctttttttaa ttttgaaggt tcttcaagac acacatgcaa
    24901 taatgcttca gcatttaatt taaagatctt ttcgaccgtt ttagctttgc agtggactgc
    24961 tatatccgct aatgtaatat cttcaacaaa ttcaaagtat tcgttactat ctccactcat
    25021 ccttttattg aacctattgg aatcatacgt gcaacaggaa tacttaaacc cgccctggta
    25081 attgcatcaa taacttcatc aatatctttg tatgcagcac ctgcttcctc agctaaacca
    25141 ctgaaagacc gcgttctcac tgtaattccg cttttttcca tctggttagc taaatctctt
    25201 cctttgaaac gctttttggc ctgctgtcgt gacattactc ttccacttcc atggactgta
    25261 ctatagaaag cttcttttgc catgggtcca ccaaccagaa tatatgaagc agattgcata
    25321 ctgccgccaa tcagtactgg ctgccctatc tccctgtaca attccggtat accctgcatg
    25381 cctttggtga atgctcgtgt agcacctttc ctgtgtacca gtaatttttt attcttacca
    25441 ttaatatcat actgttctat ttttgccgta ttatggcaca cgtcatatat taactttata
    25501 cccaattctt ctggtgattt cttaaaaatc gtgcacaata catcacgaac aatatgaaat
    25561 attaattgcc tgttaagaaa agcaatattt atggcacaat tcattgcatg aaagtaagcc
    25621 tgtccttcct ttgaatggaa tggtgcacat gcgagctcac gatcgggaat atcaatatga
    25681 tatttttgtt tcatgaccga tataaactga tccaaataat cagttgctat ctgatgacca
    25741 aaaccacggc taccgcaatg tatcatacat gcaatttgat tattgcctgt aataccaaac
    25801 ctgcttgcaa tatccctgtt aagaatatcc tcatcacgta taatttgaaa ctctaaataa
    25861 tgattacctg accccaatga accaacctgt tctttgccac gttctcgggc ttttcctgaa
    25921 acagcttcag gttttgcatt tgcaatacac ccattttcct cnnnnnnnnn nnnnnnnnnn
    25981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26041 nnnnnnnnnn nnnnnnnnnn natttcatat aagcaatttc caattttttt tgctatgcca
    26101 tttaccataa tccctattgt ttattgtaac atagtattac tctttagcta ctggctcttt
    26161 ctggttttgt ttttgctttt gtacagggat tgggctttta atcatttcac atttgccttc
    26221 aaatactaca ccatcagcta ttataagttt tgatgttcgt atattcccaa atagcttacc
    26281 atcactctgt atttcaattc tgtccttagc ttcaatgttt ccatatactg ttccttttat
    26341 aataactgta ttagctttta tatttgcttt cacagtagct ttctcaccaa taaccaaaaa
    26401 atccccagaa gcaatttcac cttcaaagta tccgttaatt tgtaatgatc tctcaaatgt
    26461 tagatcaccg taaaattctg tatcttttcc aaatacagtt gctatcattc caatttcata
    26521 taccggattc ttattaacct gaataacttt tttagccatt gtatatacct tcttctgtta
    26581 aaataaacat ttatattgaa aaattcaaac cttttacagt agcaagattg attattgaat
    26641 ttaattatac aagctaaatt tgcaaaatat atcttatttt gttgtcattg tgaaaacgcc
    26701 atcccaatca tccggtggcg gggtgagttt atattgcttg cacctttcta aatataattt
    26761 agatggcccg tcattggggt caacttttaa agctttttca aaaaaatcta tagcttcgtc
    26821 ccatctgcgc tttttatatg catctaaacc agagttgtat aaccttaaaa cctcttcttt
    26881 ttcttttgtt atcataattt cgcctcgctg aaaatatttt caatacgact ccaatcttaa
    26941 aataataaaa caattaatat ctcctgtcaa taaagaataa tgccagctca gtcataacat
    27001 tcttaaattg ggtatccgga aatactgata gaagttttac cgctctttgt acatattcag
    27061 ccgcaatatt tatacaatat tcaactacat tatattccgt ggctttttca aggacataat
    27121 tccagttttc attatctgga tgttttgcat aattgattaa taaatctttt tctttttccc
    27181 ccactatctg caataatcgt aacaatggca aagtaatttt gccatttata aaatcattgc
    27241 ctgcatcctt accagtaatc tctgcattat tcaaataatc cattgcgtca tcagtaatct
    27301 gaaatgcata tcccagttgc ataccacaat tatacaatat atctgactgc tcattatcca
    27361 tttttgcaac acatgcacca agctttgcac aggcagccat aaatcgggct gtcttcattt
    27421 caatgatacg gtaataatgt tcttcagtta tattatcaat attagaatat tgtatttgat
    27481 acagttcgcc ttttaccatt tcaatggttg catctaccat aacagggaaa aggtcggtgc
    27541 gattattccc aacagcgatt tctaaagctt tagtatacat ataatcacca gccagtacag
    27601 caacttttgg cccaaatagc ctggcaactg ttggcaatcc ccggcgtaaa tcagcgttat
    27661 caatgatgtc gtcatgaacc aaagaagcag catggattat ttcagcagca caggcaatat
    27721 caataatgtc atcagggata gaattattca atccacttga taaaataatc atggaggcac
    27781 gtattttttt tccacttttt ttaaacgtat gcatgctgct ttcgtctaaa agatcaatgc
    27841 ctgtatataa tttatttttt atagcttcat caacagcctt aaggtaatgt tctataggcc
    27901 taagaattga ctgcaaatct tttttaggtg tcatttttct gtattcctta atcaatcaaa
    27961 tgcgctgaga cattcacgtt gtgactcaaa aatttcgaat accttatcga gcatagtagt
    28021 ctggaaaagc ttaagcaggt tttcatttgt tatgactaac tttatatcac cttcgttttt
    28081 tatcttcctt ttaaaagcaa caatgactcc aagtgcagaa ctacatatat gcttaacatc
    28141 ggtcatatcc atacagatat tgctataacc ttcatctatt aatttttgta atgcattttc
    28201 cagatcctct gacatttcct gatctattct tccatctaaa gtaatgatag ccaaattctc
    28261 atctttttta attttcatat tctacctcat gtatcagata ttacttttct attatacata
    28321 attataattc atattgatta taattacaat aattattttt aaagacaact gttatagtat
    28381 caaatatttt aaagcttaca ccagtctacc attagactga gattactgtt tataaaaatc
    28441 agtctataca atcagttctt ttaacattgc ataagcaacg atacaagcat atacatgttt
    28501 tacaccattt atttttagca ggcgtgcaca ttcattaata gttgctcctg tggtatacac
    28561 atcatcaaca ataagaaccg atttatcagc aatattacta gtatcctgaa taatttcata
    28621 tttctggtat gtattaacaa accgttcatt gccacttaac tttttctgtg caactttctc
    28681 attttttata ttcaataaat ttaacacatc tatattgtac cttttaccta tacagcgtgc
    28741 cataacttca gactgattaa atcctctttt ccataaacgg tctttagata caggtatgta
    28801 agtaatgaaa tcaaagtgcc atttcattat atcaattgcc tcagtacaat actgtgcaaa
    28861 taacctggca atagatttgt tatgtttaaa tttcatagcg taaaccagct gtgttacagg
    28921 ttcttcatat ttaaacaacc atacaccatc atctaaaaac catttccgtt taccatgtat
    28981 ttcttcttct aatgaaaaaa catattcgcc ggaataattt ttaatattca gattaattat
    29041 acttttacaa ttattgcaaa caccatcacc attggatata agcttcccac aataaacaca
    29101 aaatgaagga aaaattacat ctaatagtat ataatgcagt aaagttataa atttactatt
    29161 tgccatagtt acgatgtaaa gctggatccg gtatgcctgc ttctgaaaaa cctttttgcc
    29221 gtatacggca actatcacac acaccacagg gagtaccatc tatttgtgga ttataacaac
    29281 ttgaagtcaa cgagtaatcc atccctaatg atattcctaa ttttattatt tcacctttag
    29341 taaggtttat aagaggtgtt tcaataatta ttggatttcc tttaatgcca cttttggttc
    29401 ccatttgtac tactttttga aatgctttta taaattctgg cctgcaatca ggatacccac
    29461 tgtaatcaac tgcattaacc ccaataaaaa tatgggttgc attaattgtt tctgcatacg
    29521 ccagtgccat tgaaagaaaa acaatattgc gagccggcac atatgttgtt ggaatactat
    29581 cagtatgata attcagtatc tcttttcctg atgttcggat taatgatgaa gatttaaaca
    29641 agctatccgg caaattaata acttcatgtg aagcaacgtg aagaaagttt gctatttttc
    29701 tagcaaaatc aacttcaata tcatgacgtt gaccatatac aaatgtaagt gcaaaaaggg
    29761 ataattccct gctttttgca actgcagcag cagtggcaga atctaacccg ccactgagca
    29821 ggactacgca tttgccagca gccattttct attttgtcat taagcttctt tttttgctat
    29881 acgcattgga aaatattcat aaggtttcat gccatatacc ttctcaaaat ccttttcaaa
    29941 aggacacatg gtacagcttg ccctatagac accttccaat gtcatcattg cttcatcata
    30001 tagaacactg tttttatcaa tttctgcatt ccttatttta agaaatgcac atgatttgca
    30061 cttttcagca ttttctctaa tcttgtcggt aattaatccc atgcaaatac ctcccagaaa
    30121 attgtaagta tatgaaatat aatttataca atattacaaa aatagcaaga aaaaatccag
    30181 catatttttg ataaaaaagt aataattcgt cagtaactat cgcctaaata acatatttat
    30241 aatatttaca cagcacattg aatatcagga tgtttgtgtg gattttatag ttgtttgtat
    30301 aataacaaat taattaatcg tgcaataaag tatgctccac aaaataaaaa gaaaggcata
    30361 gtaaaaaaga aaattaatat ttcttcaaat tttacgacag ttccatgcaa tacctttttg
    30421 ccaaaataat tataatgaaa atgctgtgga tccttgctgc gtttgtatat tgccctggct
    30481 atactgattt ttataaatag cataataagc attataaatc ctgtcattaa ccaaagaagc
    30541 agcttgaaat ccacctttgc aacataaact ggatgaatga ttgctaaagt ggtgatagct
    30601 ccagccagaa gtacattgct gatggtagta tttataaaac ctttgagata tttattttct
    30661 cttcgcaata catagagaac aagtgaataa atgtgtaatg aaaaagaaat ttgcagaagt
    30721 atgaaaaggg caattagcat cagtattcct gtatactatt attcagatag caaaaccctt
    30781 tttataatac agtacaatat attaatttgc aagcattctt tattatttat ttttttttta
    30841 tagcaggaaa tggttatcgt gtgcgcgaat ttatattgat aaggattcca gcgcttataa
    30901 gtgacattat taaatttgaa ccaccatacg ataaaaatgt aagcggaaga ccggtaacag
    30961 gcattatgcc caatgccatg ccaatattaa taattacatg aaaaaatatc atgcatgtta
    31021 ttccaccggc aacaagcatg ccgaatttat ctgatgcttt taatgcaatg gtgaatccct
    31081 tatagataat atacgcaaat aaaagaagta ctatagttgc accaataaag ccccactctt
    31141 cagcaatggc agaaaatatg aaatcagttg atttttcggg taaaaacccc agttgtgatt
    31201 gtgatccctt taaaaatccc ttccctatga ttccacctga accaattgca atctttgatt
    31261 gtatcacgtt atatcctgaa ccatagggat ctaaatcagg atttaaaaat actaaaattc
    31321 ttcgcttttg atattctttt aaaaatcgct gtataattac cgataagaat aaacctgatg
    31381 aaaatataaa tgatggaata tagatagccc tgtatatttt agagttaaaa aagaaataaa
    31441 gtataaatgt tataatagaa acaattaata acgaaccaga aattatatat aaaagttcat
    31501 ttcccttcaa aaatgaaaaa ataccacccg atatacttac cccactccat tccagatatg
    31561 ttagcatcat tggcacaaac agggctatag cggctatacc cacaatagat atcagctgtg
    31621 ggatgtcagc accacctaaa aataataatg ttaaaaatat tggtacataa atcattgctg
    31681 tgccaaaatc aggttgcagg taaatgagca aaacaggaat gccaataata gcaaatggga
    31741 caaatagttc acgcaatctt ttgatatcaa attgccgtaa ttccagatat tttgacagta
    31801 tcaaaatgct tgccagtttc ataaactcag aaggctgtat ggtaaaaaaa ccaaaactta
    31861 gccatgcacg agtattacgt atttttgcgc caaaaattgt tgtaatcaaa aggaaaaata
    31921 gtaatattaa atatatatac agagaatagt cacccagtaa cctgtaatgt attcgtgaga
    31981 ctgcaaacat acatcctgca ccaatgataa accataacag ttgcttttta tagagcccac
    32041 tgttcacttt ttcaattggg tcaaagcctg ttgcataaat agtaataata ccaattaata
    32101 caagagcaat aatggcaaaa aggaaattaa catcgtattc ctgaaaagct gattttatgg
    32161 ttttgaacat gttaaaaata tccctttttt attagtgtgc tgaatacctg ttcagcaacc
    32221 ggcacagcag tagctgctcc tgctacacca tattcaacca tgacaactat ggctacaacc
    32281 ttttccggtt ctgcatcata cggaccaaaa ccaacaaacc aggcatgctg acttgcatct
    32341 tcctgccttt tggaacgtgt ttgtgcagta ccagttttgc cacacatttg cactttcaga
    32401 tatcctaacc gtgcagcggt accacttact acagccattc tcattccctg tttaatagtt
    32461 tccagtgttg ttggcgataa aggaatttcc cgtaattttt cgggaacaac aactgaaact
    32521 atagaagaat tatcatttga acgaatctct ttaacaagat ggggtttgaa aacaacccct
    32581 gaattaacaa tagcagaaat aaaatccatc atgcccatag gtgtaaccag cataaaaccc
    32641 tggccaatgg aaagatttac cgtatcacca tcaaaccatg gctgaccaaa ggtctttagt
    32701 ttccattttt ttgaaggtat gaatcctgta atctcacctg gtatatcgat acctgaaggt
    32761 tcagctaaac ctaaatagcc tgcataatta aagattgtag ttggccctaa ttttaatcca
    32821 agctgataaa aatatacact gcatgatttt gcaatagccc acaataaatt aactgagcca
    32881 tgagcctgat agcaataaaa atccctgtct acatatcctt tcaaggtata tttaccaggg
    32941 caatataatg ttacattggg gttccatttt tcttcttcca gtgcagcaat tgcagtaaca
    33001 agtttaaagg ttgatgcagg ggggtatcgg gactggatag tcctgtttaa aaatggctta
    33061 ttcttatcat tattcaactg cgttattatt tttgcattat tttttgatat aattagattg
    33121 gggtcataat ccggcttgct tactaaactt aaaatttcac ctgttgcagg cttcataaca
    33181 ataacagagc ccctgttatc gcccaatgca gtataggctg ttttctggac atcataatca
    33241 atcgtaagaa taatattata cccgggcttg gggtgttggc caatttcttc tccttcagtt
    33301 ctatttttgg catcaacaat acggcgtaca taaccgtcaa cacctcgtaa tgttgaatca
    33361 tactgccgtt caattcctgc tttgcctatc ttctgataat atttgtatcc aacatcctta
    33421 taggtattat attcatcttt ggttaagctt ccaatgtatc ctaacacatg cgaaaacatg
    33481 tttgcaaacg gatataccct taccggtgca tcttcccaca taatgtttgg aaatttatcc
    33541 tgatttgtgg cgatagttac tacaatatca aacggaacat cttcctgaat aatatatctg
    33601 ttatacggat tatttgactg tatatccgca attatctcat catagggaag ttttaaaagt
    33661 gcacatacat tttttattgc ataatataat gtttgttttt cctgataccg ggcgggaata
    33721 acaataatat taaaagacgg tctgtttgtt gccagtacca tattctgttt atcaggagca
    33781 aaatttctgt cataaatttc accacgggat gcaggaatag gaatattgtt ttccatgttc
    33841 atacgcgact tttccgaata ttccttcccc tgaattatct gcagattaac aagctggaag
    33901 aaaaaaatac caaacatggc aataacaata atgccaacaa aaaaaagccg ttttctgaac
    33961 tcttctataa tctttgttct tattgaagca aattgcatat cttaacgacc ttccctggac
    34021 aactcctgtt caaatatctt atcaaaaagg aagaataatg gtggggataa taaggcatta
    34081 taaaatgatt caggtaaaat aattgaagtt attgaggata atgatccctg atggaataaa
    34141 taacataata tcagtgttat aacaccttta gcaatcgaag caaaaaagat taaaagaaaa
    34201 acagtaaaaa gattttcttt aaaaattgac ctcccaaaaa aaccaattat ataccccaga
    34261 gccattttgg ggaaagccag caaacctaag ggggaattgg aaattgcatc atgtaatagt
    34321 ccaccaataa atccagtaac ctcaccatat aatgagccaa aactataggc aaaatataca
    34381 acaactataa aaattaggtc gggtttaact ccacctattc tcagaacatc aaaggaagaa
    34441 tgtccctgta ttattagtga tgctagcaat agtgctcctg ttattatata cattaaaatc
    34501 atagttgtga atcatcctgg attaatttta aaatctcttt atctggctct tttttaatga
    34561 caacaacatt ctcaataagg tcaaaattga tatatggctt tacgagaaca cgctgaaatg
    34621 caccagattc ggatgggaat gatttaactg caactcccac aagtaatccc tgagggaaca
    34681 caccaccctg accagacgta ataacgatat cacccggctt gatagttgct gttttatcaa
    34741 cataatcaat aacacataat ttaccaatag ctgaataccc acttaaaagg ccgggatacc
    34801 tgctttcctg aaaaacaaca ccaattttta aatcaggaga aataattggc aaaatgcgtg
    34861 atattgaacc tcgtacttct ataattttgc caactatcgc tttttcatca ccaaagaatg
    34921 caactactgg catattgact tctataccat cagaactacc tctattgata attatcgttc
    34981 tgaaccagtt atcaggatct tttgatatta tagtagccag aactgtatca tactttagac
    35041 gttgttttag gcctaaaagg ttccgcagtt gctcattttc tcgttttatc tcttcaagct
    35101 cttcagcaac agattcatac tgctgtagcc gtaatcttgt tttctccagt tcttcctgag
    35161 catttcgcaa ttgcgaaaac cctgaccata aaagggatac attggtttga acagcatggt
    35221 ataatttctg gaaagggaaa ataacagcat taccagctcc ctccacaaca tttgtcaccc
    35281 ctgacgattg taacgagagt gatataaaac aaaatagagt aaagagtata aataatacaa
    35341 ttgttctatg ctttataaaa aattccacaa caaacccatc attttaagta taaaataaat
    35401 actcctgcat tatatgcaag agcattttta tatagaatga attacagcca gaactacttt
    35461 aagtttgagc ggtaaagatt tttaatttcc tctaagaatt tgccggctcc cataacaaca
    35521 caggttaatg gattttcagc tcgtatcact gggacgcctg tttctttgct gatatactta
    35581 tcaagacctt taagcagtga tccaccaccc gacagtacaa ttccacgctc tactatatca
    35641 gctgcaagtt cagggggagt gcgttccaat gtatgcttga tagcatctaa aatctggtca
    35701 acaggctctt ttaatgcttt tcgtatttca gtagaatcta tctccaatgt ccgtggcaaa
    35761 cccgagatag catcacgtcc ttttaattcc atagtttcaa tcttcttttc agggaacgca
    35821 ttacctaacc tgaatttaac atcctcagcc attcgctcgc caataaccag gttgtactga
    35881 gttcgcatat attttataat tgcctcatca aattcatcac cagcaatcct tactgaatct
    35941 gcaataacca taccacccag ggagataaca gcaatttctg tagtaccacc accaatatca
    36001 actatcatgt gcccggctgg ctcatgaatt ggaatattgg cacctatagc tgctgccagt
    36061 gcttcttcaa tgagatatat ttcccgggca ccagcctgtt cagcagattc tctgactgca
    36121 cgtttttcaa cttcggtaat acctgatggt acatctataa ctacccttgg gcgaaccagg
    36181 gtagtacgtt tatgcacttt ctgtatgaaa tatcgtatca ttttttctac agtttcaaaa
    36241 tcagcaataa caccatcttt cataggtcgt attgcaacta tatcaccagg agttcttcca
    36301 agcatcctct ttgcttcatg tccaactgct aaaaccttac ctgtgctggt ctggacagca
    36361 acaacagaag gttcactcaa aacaataccc tgccctttta catgaaccag cgtgttagca
    36421 gtccctaaat ctatccccat atcatttgaa aataaactat aaacagaatc gaagatcata
    36481 ataagtttac cttttagtca aaaaattgat tatccgaata ttatgtgcaa ttgaatgata
    36541 ctttacggcc gtttttgtca agcgattatc attaaatttt tttatcaaaa attaaaaaag
    36601 attacattat ccccagtaat cgctttgctt cggcattttc tgaatcaagc aaaaggacct
    36661 cactccacaa cgcctttgcc tgagatacat tccctaccgc tgaatatacc tttcccagcc
    36721 aaagtttaca ttgtacatcc tgagcatcaa tctgtaaggc agcattaagg tgttcaatag
    36781 attgatcata aactcctttt ctataaaata tctttcctaa ttcaatatga acttctttaa
    36841 taatggtatt atccctggaa ttttgaagta tatttctata ctgcataatg gcattgctct
    36901 gctgattggc ataaagatat gatgcagcta aaaacattct tccttcaata ttatctgaaa
    36961 ccccttttgc ttttacaagc tcaatccctt cttcataatg accgcttttt atggctataa
    37021 gcgaatacat tctgatatta tgtaaggata acttctgtat atttgaaact ttttgaataa
    37081 tgttatatat ttcattaaca ggataatacc cacttaaata caatgatatg gcctgaataa
    37141 cccttgtatt gtcatcaatg gaagaccggc cgcccagagc tatcccttta tttatggctt
    37201 ttatagataa aagaaaatac tggcggatat tagcagagat attctgcaac gtcgattcat
    37261 catataacga ataaatggat ttatttaaaa gactatatgc ataatgacag tatgtttcgc
    37321 ccagtaaata atatgcatcc gaactcaacg gattgctttc agcatattta tacgctgcat
    37381 tgcagagatc ttttaactgc tgctggcgga tggtactatc tggctgcttt gaagctatct
    37441 caatcttttc ctgtaattta tttattgtat atttccagaa gaatatatat tgatgatact
    37501 gatgaacaac atacacagca gcaattatta atagtaaatt aattacccat ttcgttgtaa
    37561 tttttcttct ctgttttgat ttatagcgat agtacatggt aaaaatcacc cttaacatta
    37621 agatttgata aaattggaat attctttttc aatattcagt aaaatatcag actcttcatt
    37681 aagaactata caattaacag tatttttacc ttttattgaa aataacatgt aattatcgtg
    37741 tgctttcatt gaagcaatac ggtaaaagtc atagcgatat aatttattat caattgtaat
    37801 gtttattccc tgctgtacgt attgcaaata acctgatcca acccttgtta aaaaactcca
    37861 atcatgtgaa gttagattat cagaaacaga gccaattgtt ccactactat caacatacag
    37921 aataacctca tacccttttc tggcatcttg agattgcaat acattttctt tatcgttatt
    37981 cttaataaca gaatcttttt caggtctagt tgaaatgtca ttggttatat caatatcaac
    38041 atctggtaaa tcattaaaat cttctttatc cgtttgagaa tttaatgtgc tcgttgtttt
    38101 atgatatccg ggtgtagata catcatcatc aaaattaggt acaaatctat catgagggat
    38161 tgttttttta atagaaactt caaaaaactc agaatacaat ataagtaaaa cgcctgtaat
    38221 gactgatgca actgctgcat ataatagaat cattgtgcta tcccataaga taaagtcact
    38281 ttgaatatta aatcagtata agaaatgtca gtaaaagaat atatgaacca aaaaagttac
    38341 tatgcaaaat acatttgcct ataaatattg tcgaaattat aaatttacta gtcaaggatt
    38401 aattataaaa attatggtat tttccataaa ccagctttaa ggtatggctg gcttttgtat
    38461 gggaagtctt ctggttcatg ttccagaaac tctaatttcc aatgttgtgg atgcaatgaa
    38521 cggtaatcac gtacactcat ggaaattgca ttgattgtag tcagggcata accgtctttt
    38581 gtaatatgtt caattaatgc aagataatcc ctgtaatgcg ttttaacact gaaattttgt
    38641 tttttatttc ttgaaaacgt tggtggatca aatattacaa aatcaaaatg tgctttttgc
    38701 tttatatgct tttttaaata ctgcctgcag tccaatttaa taaaatctct tgaatcatac
    38761 tgaatatcat ttaatacata attttgtttt gccctgctta atacagtttg tgagatatct
    38821 acattaactg catactgcaa cccatttttt aacgaatgaa cagaaaacaa ccctgtatag
    38881 caaaaaaggt tacataaccg cccaccgttg tgataatact taaccattgt ttcccgtacc
    38941 ttccgcatat caagaaacac acctgtattc tgtccttgta taagatccac ataggctata
    39001 atattgtttt gtttaattgc aaaattctca ggcggcattt ctccatccac aaggatgctt
    39061 gtacgcagct ttgcaatttg ctggggatcg ttaactttgg ttctgtcttt gagcaaaatc
    39121 ccgttaacgg ggatatgtaa tgaagaaagg atttttttac aatcattaca tattttggag
    39181 tagtctttat acaatgaact atcgtacagt tgtaacaaca caaaaccatt ataataatca
    39241 ataataaggc cttcacaact atcaccagca ctattgaata gcctgaaagc attggtctga
    39301 gtgcttttaa aaagatgttc gcgcttaagg atggcctgtt ctatgatttt aaatgagggc
    39361 atttactgta taatactatc taaagtttct ttaatacgat cttcaagagc atgaaaatcg
    39421 tttgcatcaa tcaaggtact atcgactata tacgacattc cacaggcaat aacatttgga
    39481 attgctaaat actgctttat gctggaaggc ataattccgc ctgttggtat aatagaaaac
    39541 gaatacttta caaacggctc tgtaatggct ttaagatact taacaccacc agcatattct
    39601 gctggaaaaa attttaatat agtacaaccc aactgcaaag catgagccag ctcagtgggt
    39661 gtagtaaagc caggtatata ttgtatatcg ttaaatctgt gcgatagctc tattaatgag
    39721 tcgctgatgg aaggcgaaac cacaaatgta gctccggcat caattgcggc tttaaagtca
    39781 tcatgacgta acacactacc tgctcctacc agcatttgcg ggaatgattt tttaagtgca
    39841 gatatacacg ccagtgcctg agtagtccgc agtgtgactt caatacagtg tatgccatgt
    39901 tgatgtaata tttcagcaat ggttactatt tttgaactat cgctaaaaac accaattgga
    39961 ataactggaa ctgtaagttt gaactgcatg gcatgcaaaa acttacaata aaagccgtgg
    40021 ataccgcaat tctatcttgt tgccatctgg atcagcaaaa acaaccagac gacctttacg
    40081 aatattctca ggtccaaaaa ctatagggat atttttttct tctaattcat caagcgcatc
    40141 atcaaaatct tcttcatcaa tataaaagca tatatggctt tttgagttgt cgggagcttt
    40201 gtagtctttg acttctacaa gtttaatcaa aatatcacca acttttatat atgcttcact
    40261 tgaattgcta accctttcaa ccacttcaaa atcaaataaa tcattataaa attcaattgc
    40321 cctgtccaga tctgatacag tgatgcctat gtgatcaaca ttttctatta ctatcattta
    40381 tgcctctcct tttcaatttc tagatataca aaattactga tattgttatt tagtattact
    40441 attggtattc atattcaacc acgttttata actctttgaa gtattgtcaa ttatttaaaa
    40501 ccattaacat gaaaaatatt ttcattttta ttcaacggta acactttttg ctaaatttcg
    40561 cggctgatcc acattgcacc cacgcaatac tgcaatatgg tatgccagaa gctgtaaagg
    40621 aataaccgtt aaaagtggtg atacaatctt acgggtttgt ggaacataca acacatgatc
    40681 agcatatttt ttaatgtctt catcaccttc ggtagcaatt gcaatgattt tacctttacg
    40741 ggctttgact tcctgtatgt tgcttataac tttctggtat acctcatcct ttactgcaat
    40801 aaaaacaacc ggcatatttt catcaataag ggcgataggc ccatgcttca tttccgcagc
    40861 aggatagccc tcagcatgga tatacgaaat ttctttcaat ttcaaagcac cttcaagagc
    40921 cacaggaaat tgtattcccc gtcctaggta cagaaaatta cggtgctctt tatataattg
    40981 tgcaattttt tttatttcat cattcttatc aagaatctgc ttaacatgtt gcggtgcatt
    41041 tatcaattcc tgcaaaaact cccttcccgc atcagcagtc atatactttc ttcgtgcaaa
    41101 ataaaatgtg agtaatatga gtacggtaat ctgcgaagtg aatgctttgg tggaagcaac
    41161 accaatctca ggacctgcat ggatatatac accaccatca ctttctcgtg caatggtgct
    41221 tcccacaaca ttggttatgc caagcacttt tacgccacgg gcctttgctt cacgaagagc
    41281 agccagtgta tcggccgttt cccctgactg gcttataaca ataactaaat cgcctttatc
    41341 aagtattgga cttctatacc tgaattctga tgcatactca acttcaaccg gtattcgtgc
    41401 atactcttca ataagatatt cacctattag accagcatgc caggatgtac cacaggcaat
    41461 aaagataatt ctccttatag cattcaactc atcttcagtt aagcgtaagc catccaaacg
    41521 tgttttacct tcatcaagta atattcttcc tctaaaagca ttctgtatgg tttcaggctg
    41581 ctcaaatatt tctttaagca taaagtgggc aaaaccctgc ttttcaatat cagcaatatc
    41641 ccaatcaatt ttgtcagtat gtttctcaac tgtttgcttt tttaaatcaa aggttttaac
    41701 cccattcctg gtaatttcaa ttatctcgcc atcagataca taaatcacat ttcgggtatg
    41761 ttccactata gcgctggcat cagatgccac tatcatttcg ccatcaccca ctccaatgat
    41821 taacgggctt ccattacgtg cagcaaccag tttatccggt tctttttttg aaatcacaac
    41881 aatgccatat gtaccctcaa ctttggttaa tgtcttctga actgcatcta ccagtggcat
    41941 tgtttgatga tagtactcaa ttaggtgtac cagaacctcg gtatcggttt cacttttaat
    42001 agtatgatta tgctgtaaaa gaattttttt aatggctgca taattttcta taatgccatt
    42061 gtgcactaac gctatctcac cactgcaatc agtatgagga tgagcattag tatcatttgg
    42121 ctcaccatga gtggcccatc gtgtgtgccc aatcccagca gaaaacccct taaaatcgtc
    42181 ataatttaaa atggcattga gctcagaaac tttacctttt tgcttttgaa caatgagctt
    42241 gttattgagt gcagcaatac ctacagagtc atacccccgg tattcaaggc ggcgtaatcc
    42301 attaataaga acttctgcta catttttatt gccaatatag cccacaatgc cacacataaa
    42361 aaattttctc cttatgaaag tagaatataa ttttaatgtc ttttctaaac catagagggt
    42421 aagaaaactt atttttatta cagtattgtc attttgaatg tgtccaacaa aatatgtgtg
    42481 cgtgaagaat taataattaa attcttcttc cactttttcg ggaacataat atgctaagtt
    42541 ttctcaccac ttcttagagt gtaatgataa tgaataatat aatcacctaa aaagttttaa
    42601 acgatagtat tttctttaaa gagctataca acgatattgc acgaacatag ttttttcatg
    42661 gttacatatc gccacttacg ttgctcttaa tgacttaatt ataaactatt tttttattaa
    42721 aagcaaaaca ttttttatat ttgttattga ttaacagcaa tggggctttt taggccccat
    42781 tggtattata cttcaactat tgcttcagcg ggacagacat caacacatgt accacattct
    42841 gtacaagcat cctggtctat aacatatcta tctttgcctt cagaaattgc ctgtgacggg
    42901 cattcggcgg cacaggttcc acacattgta cattcttcgg tgattttata agccatgtat
    42961 gtttcctcct gaaaatatag cattaacttt acgtataaca attaaaataa attagtagca
    43021 caatttttat tagaaacaaa atttatagag ataataatac caatattgtt aagaatataa
    43081 ataaattatc attgatagta cctgttaatt atgtcttcct atcatttcaa tgaaattaaa
    43141 aatatatgtt tcttttacaa ttatatttta tatatttttg aaaaaaaatg cttatatata
    43201 ccttttagtc ggtttttaaa ttgacttttt cattggctta atctctttat tattatagtg
    43261 atatgttgta attaaatgag tgattattca ctcatcatgc cattgcctgt atatctaatt
    43321 ttaattttta taaagggggt tctatgaaat atatttcgaa aattagtatt gtattattac
    43381 tagtttttac atgctctgca gtttttgcag gccaatgggt atacaaacca atgagtatta
    43441 atgcccagaa aggtgatatc atattatcaa ccagtccggg ttttattatg gaccttctgg
    43501 caattttagg ttgttactgg agccacagcg ggatggctgt tgacaatggc ttcaacattc
    43561 gccataacac catgtatgtg agcgaagttc ccattgaata taattatatc tggtttatta
    43621 aaacaacacc aaaacgtatg gatcctaccc gtttgagcaa tggattgcca ggaatactta
    43681 ctgaagatat tgacacaact tacaacgtaa caaaaaattt ccatgctgcg ggcggtgctg
    43741 tattaaaacc aacagctgca aatgaagcat tatacagaca gtatttgcag ttagcagccg
    43801 ataagttgct gtacgttaaa gcatattacc gggttaatgc atatgtcaat atgtaccagc
    43861 tcgattacgt aaattattat ataacaggcc gtggtaacca ctgctccggt acatgctggt
    43921 atgccaatta cttcgcaggc aaaacaatga atgttgccta tatcccacct tcactggtaa
    43981 cccaatgtgc ttacaatctc tataattcag taaagaatat ggtacgtgat gaagccggcg
    44041 gatttggtgc attcatcata gacattgaag gcctctttgg cactggtgct gatgaaaaga
    44101 tagcaaatca gattgttaac acgtttggct gggacagatc ctgggacact tcttcatact
    44161 ggagaactta tataaaccag aaatccgcaa cagctaatgc tcctgatcac ctgttgctgt
    44221 acacatatac caatccttct ggatataatc ctggtgtaca aactacctcc tcttcatact
    44281 atggacaggt tgatccactg gttattacct ccggatacta ttactgggtg gattaactgc
    44341 atgaacacaa caatcccggg tgccattttg cccgggattt ttttattgat taaattaatt
    44401 ctttattatg atctatcaca aattaaaata gtatagctat ttcataatct tataacacta
    44461 ttgtaaaaca caggatacca tctatgaaac gcacatacat tataaccgga attattattt
    44521 tttgtattgc agcatttgta ttaatactat ctgttaataa aagcaatcaa caggatgata
    44581 cttcttcaaa aacacaatca ctgcttaatg tatttcaatt catgtcagca cctgaaccac
    44641 aagaagaaat accaccggtt gaccctgaaa aggttgaaac atatgagatt gagcaggcaa
    44701 atttcattaa agagataata ctggaaaaaa atccagtatg tgcaggggaa gattttaaag
    44761 ttactgttat tgcaaaaaat ccttttggcc ctgatgcaca tttagtgtat cgcatcagca
    44821 acaagctggg taatcctgca attttacggt ttaccaaggc aggattacgc gaattctatg
    44881 taacagtacg tgatgaaggt aaacacattg atatgcgtaa agttcagata aatgttgttg
    44941 attgtgctga taaaccaata attacattga gtgcacggtt acataatctg gaacctgaat
    45001 cagcaatctt tgaagtcact tcacagaaag ggctaaccgg taaatgtacc tatgattggg
    45061 attttggcga tggcacatac gcaactacgc aaaccggtta tattactcat agctatgcct
    45121 cacgtgacca gaaaggattg caatcaagct ttatagcaca ggtcaaggta actgatgcta
    45181 acggaatatc tgcaattggc agaggaagca tatcattccc caatgtccat tatttatcaa
    45241 atcttatggg aaatcctgta ataccagttc tttacaataa atttccctcc attgataatg
    45301 catacatcac tgttgaaatt aatatgaaaa atatttttga tgaacctgta gcgttcaata
    45361 atgccgtaat tgaatgcaag ccatgtaata ctgcactttc atctgattat aaaaatatta
    45421 atcccgacca aattttagaa tcaacattca tagctccaaa atcttccgtg aaacaattac
    45481 tgaaagtgcc aaagtcatta ttaccaaagt ctacatgcaa tctcaatatt accttatcag
    45541 gaccatatcc agataaacgg gaagtatatg caaagatttt tctgaatata cctccacagt
    45601 caaaagatga tattgacaca acacgggata aaattatcac cgacgatgaa atgatacaaa
    45661 agctcaataa agcagctcaa attctgggga aagataggcc tataacacca caggatatac
    45721 agaaattaga gcgtgaaggt aaattataac atatttatgc taacagtata aacagcaata
    45781 taccaattac aattaaacct gctaaaattc caatcaccag gcttgctttt gaagaagtgg
    45841 cttcttcatc ggaaactgca agtctggttt ttacttttat atcctgttct tctattgctt
    45901 tgccataaag gctttcggta attttgacaa aaagagtata cccctttttc ttattataat
    45961 taactgaata cacttcagct tcaattggcg tgtttttatt atcaacgatt aaattaagtg
    46021 tttttgcaac gtcatatcca tacggggttg atgtgtcaat taatacataa ataaaatcac
    46081 ctttttgtaa ttgtgaaacc tttttgcctg aagttggtga taatactaaa tcaacagcaa
    46141 ccagcttttt atcaaattta ctatcgcctt catgtacttc ttcctttgta tcaaattgtg
    46201 atggattata cagcatatgg aatctgataa catctgtgcg gtgcaattca gcacgcacct
    46261 gaatatcttt ggattgctgt aacaaagatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    46321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    46381 nnnnnnnnna acaatttgcg ggttgcacaa ttgacaaagc aaaaacaaat accgctaaat
    46441 tgacttgaat caagataaag cattgcaata ccaatatcct gtttgccttt ttttatagat
    46501 tcctcagcta cttgcctgtc attatcataa tatggtgcaa cgatttctac ctgctgagac
    46561 aatgttaatg aatcaatttt ctgggtaata attatttcag tgtattgagc atttgaaata
    46621 ctatcaaact cagaatacac atcaactata tcaatattta aagctgactt aatgatacgt
    46681 tgtacttcat ttactgcagt tattgggtta ttttcaatat tgttaagcaa tacagtgata
    46741 tttttctggt ccactctttg cagaagataa tttttaataa cttcaaaata ttctttttga
    46801 acagcatact gctggtttgc tacattaata gcttcaataa aatcaataga tgaaacgtta
    46861 gtacgagcat tttttaaatt atctcctgca ataaaaaccc cgtttatacc tttaattctg
    46921 ttattttctt tatcaataac tgtcaaaagt aagccagata tattgagttt agcacaatat
    46981 attttacttt ttattgcaat aagtgttttt gctgacatga ttattaacct tatgaaataa
    47041 tttaaacaca catttcaagc aaaagcaatt cttttttatt aaaataattt tttcatatta
    47101 ttgtaataaa tattttcact aaaatattgt tgacgttttg tatatttcag catttataat
    47161 atagtatcat tataacaaat aaacagggat caaacacaat gaaaaaaata atgcctaaat
    47221 taaaaaatag aatattggaa tttaaaaatt tcattatcca gcttattaat cagatttacc
    47281 gtggtgaaga agccttcaac tggctaacaa ccaagataat taatatgatt aaagtattta
    47341 ttgtagcatc caggaagttt atgaaagatg attgtcttac caaatcatct tcaattgcct
    47401 atacaacaat tgtttcactt attccaacgc ttgcagtggt acttaccatc tattccattt
    47461 tttcaggtgt gggcgataaa aaagatgaac tgttcaggca aattacactc tttcttgttg
    47521 aacataatat taaaataaat gttgatcctt tttttcagac aataagcaca ttaattgata
    47581 acgcagccaa aataggcggc ataggcgccg tggtcttagc tttttcagca actgctgttc
    47641 tcagaaccct ggaaaaatcc cttaatgata tcttcagggt taccaaacag cgctctatgt
    47701 ttttaaaggt tatctattac tgggcagcat taacactggg cccaatcatg attatagctg
    47761 caacagccgt agcaacacaa atctcaaata cactgtcagc tccccatctt caggcgatag
    47821 ctcattataa caacacccat tacatagtgg gcaacaaagc caccatactc tttaacaata
    47881 aagatgatct taattttact tctatgccca ttgaaaaaat tgattttgat aatcaacaca
    47941 ttttttcata taatcccaat gataagcagt ttaatgcaga agaattcaga atagagatta
    48001 ttgaatataa acgtactaaa ttttctgaca ttgcctttgc tggccagaag gggtttatta
    48061 ttggtgataa tggaattata ctcaaatcat acgatgctgg caaaacatgg cttatagaaa
    48121 aatgggggaa ttttactttt aacgatatag aaatgattga tgacaataca ggctttattg
    48181 ctgcaaacaa tgggtatctt ttaaaaacta atgatggtgg ccggtcctgg caggtaatgg
    48241 agtgggaaaa tgtaacatca aattttaatg caataagttt ttacaaagat aaaggaatta
    48301 tagttggcga tagatcatat atattaacaa cttctgataa agggaaaacc tggcaaatac
    48361 aacagcttgc tgaaggcaaa tacaaaaaga atttccttaa tttcaacgat gtccagataa
    48421 ttgatactaa cacatcaatt attgtggctg atgaaggatt ttatttaatc agttcaaaaa
    48481 attttacctc ctggaccgct cacaagttca aggataacaa cctgtatgca gtttatttca
    48541 ttaatcaaaa cacaggtttt ctagcaggtg aaaaagctga tatttataaa actatagatg
    48601 gtggcgaaaa gtggacagtt aaaaaactta aaggtgaacg tatcaatgaa ctatcactca
    48661 ataataatct gttgtgggct attgggaaca atagctttct catgctatca aaagatatgg
    48721 gtgtaacatg gcaggggctt aaagggagca gtttttttgt gtatttgtta aatttctttg
    48781 caccattctt ctttatctgg attctctttt taatgtgtta tatgatatta ccaaacacta
    48841 aaataccact gcggcccgcg gccataggag catcatttac cagtgctgtg tgggttatct
    48901 tcattctatt gtttattgta tacattaaag catttgccaa gagtacgttt gccatatacg
    48961 gcgcacttgc tgcattccca atatttttac tggtagtgta ttcatcagcg gtgatcattt
    49021 tatatggagc tgaaatttca tacgttttga tgaatccatt aagctataaa tatttaaaca
    49081 gggcattgaa ggataagaag gatattcatg tttattcagc tatagccctg ttacaccata
    49141 tatataaaaa atttgaatcg ggaaaaggcg caagcagttt tcaggaattg ctgccaatga
    49201 cgctgaacga tgttgacctg cttgatatgc tgttagacct ttttatcaaa gaaaagttta
    49261 taacacacac tgaagaattt ggaatcattc cggcaaatgc aagcaagaat atcagcatag
    49321 ctaaaattat tgatacaatt tacagcatat cactggatat cccggttacc aaacaggata
    49381 aattaaaaac gtatttagct gatttattca aggatatgaa gcaatcactg gattctattg
    49441 tcaaaaataa aacgttagct gatgttatac aagcaacaga atgaagtgta ttgactattg
    49501 cgaaactatc ctgaataaaa atgggattac tattactttg ccctgaattt catcaggtac
    49561 tttgccaaag ttttttgaca accgtatggc atcaatgcag gattcatcaa tagctttatt
    49621 ccccaacgaa tcaaccagaa caatgtcaag aacgttacct tcacgatcca tggtaaaata
    49681 caatttaaca tcctgagtag gcactgccat tattctcatt cttcccggtg ccatacttcc
    49741 tgtcattgga ttatatccat atattactgc attagcaaga agtggtgggt gccagttggc
    49801 agccacttta tctttcatat ttttgaagta ttcaaaaggt ttgaatttta ctgtattgaa
    49861 agaaaaaaat ccatcactgg taaaaaacag cgcatttttg ggtgttatgc tatatttgtc
    49921 gggtatgcgt gtaaattcct tatcgccttc aaattcaggt actccacctg cttcatcttt
    49981 aaaaaatgta atgctgtatt ccgtattttc ctgtgcaacc atgattttat ctttagcagc
    50041 tgtgctgctt gcaatcccac ctttacctaa ctgccgcacc cctttctgca tcttaaaatc
    50101 cagagagttg ttgagccacc tatcgccttt ttcttttgtt atatagcctt tggcttttga
    50161 cgtcttatct gaaagaagtg tatttttatt tatatctttt acatcatcct gattgatgtt
    50221 aacaatgata tccctgcctt taaacaatga atcggcttgc tgttttgcaa acgcaccata
    50281 ccgtatctgt gcaattaaaa aagaaacaat cacaatagta tgcatgaaaa acgaaaccag
    50341 aagtacaagg tcaaatctta tttcggcacg ttccatcttc caatcccttt tctgtatatt
    50401 tttgtttgac tacgcacctc ttccagcagt ttacgatgtt cagcagtaaa ctccacattg
    50461 cgacgtgaca tcattgtttt gcatgttgca aaaaatttat cctccgaagg caggtcaaca
    50521 atcttaccat caatatacca gctgaatggc ggtataaact ttggtgtgat tgcaccattg
    50581 tgaatgacca tacaacccgg ccccatgaca gtaccggtgt tgattaaggt cccaatactt
    50641 gtcatagtgt aatcacctat aaaacatcca accttaagca atcctgtgtt tatcttctct
    50701 tctcctataa ataatttaac ggttgtatag ttatttttca aatcactgtt ggtagtcatt
    50761 gcccctaaat ttacccagct accaatatat gcatgaccta taaatccttc atgatattta
    50821 tttgcaaacc cttgaaaaat acattcttct acttcaccac caaccctgca ccaaggccca
    50881 atgctgcaac cttctctcac ttttgcctgt aatagtattg tattatctgc aattgcgcat
    50941 ggtccttcaa ttcttgtaaa agattttatt gagcaaccct ttccaatata cacaggtcct
    51001 tttgtacaat ctataactac atacggttct attgttacat catcttctac tattaattgg
    51061 gaactatcgc ctaaaattgt tacactatta ttccccctgg gattttttat taacttaaaa
    51121 tcctgtgtta taatatcccc attgttacca atcaattccc acaaagatgt cagcacataa
    51181 cccttatagt tttctactac agctttgttc tgtaaaaaat tttgtacatc ctcggtggtg
    51241 gtgaattgtg gaatggcagc ttcatgtaac tttgcaagta caggaatacc gttatgcata
    51301 taaatggtat tatcatgtag gtccgatagc tccgggtata taacccgtgc attgataaac
    51361 agcaattctt ctgaatggtt cattttcacc ggttcattaa tatgtaactg tggatactgc
    51421 atatgagcta tcgcttctaa atgatggcgt gtataaaaat agattgagtc aggaaactgt
    51481 gatgggaata atgtggcata tcgatagtgt atcgtgaaaa ttccacagcg aatatcccat
    51541 accggccttg taagggttac tggaaaaaag tcattgtact gtgaatcttc aaaaattatt
    51601 attttcatgt tttctttgta acagttgcat taatattaaa tcattaatct tcaaaccatc
    51661 tattcataat tataactgtt atttaaatta gttataatct aaactgttgt caataatatt
    51721 atgtaattaa atatccacta cataccccgt ggccaaaggc ggaggatgta atagtcaata
    51781 ctataccaca ataaaaaagc caccctctaa gagtggcttt gcatgtataa acgttgagaa
    51841 aaagctattt atttgcttct tcttcagcaa attccttctt gcgctcttca ataacctttt
    51901 cagcaatcct gcctgaaatt ggctcatagt gtgaaaaccg catttcaaaa gtagctttgc
    51961 cacttgtcat tgcacgcagg tcaattgagt aacgcagcat ttcagccagt ggcaccagcg
    52021 ctttaaccac cgatattggg gatttccctt cttcatccga acttcccata cccagtacct
    52081 ttccacggcg gctagtgatg tcatttaaaa tatcacccat tgaatctttt tcaacataca
    52141 catcaacttc catgattggc tctaacagta tgggacctgc tgcctctaaa ccttttttca
    52201 atgcctgccg cgctgctatt ttaaatgaca tttcagagga atcaaccgga tgatacgaac
    52261 catagtaaag ctcaaccgaa acatctacaa ccgggaagcg tgcaagcaca ccctcatcca
    52321 tgccttcgcg caatcccttt tcaacagccg gtatgaagtt acgtggaata acaccgccta
    52381 caatgctgtc aataaattca aaccctttgc cacgttcaag cggtgcaact ctgataaata
    52441 cttcgccata ctggccatgc ccgcctgtct gctttttgtg cttgtattga gcttcagctt
    52501 ttttggtgat agtttccctg tatgcaatct ttggctcaga agtaactagc tcaattttat
    52561 tcttttcttt tatggatttt aatataatat ccagctgcat ctcacccatt cctgacagaa
    52621 cggtctgttt tgtttcagga ttaaatatat aggtgatagt tgggttttca tcagttattc
    52681 gtgagaatat ctggccaatt ttatcttcat cccctttctt ggatgcctgt actgcatatg
    52741 aaaatacagg atttggcaac tgtattattg gcaacaagac tgaatgcttt gtatcacata
    52801 atgtatcaag ggttgctgtc ctatccagtt taacaacaac accgatatca ccaggaagaa
    52861 gttttgacac ttcaaccggt ttgttgccaa tcatcgtata gagttttgta acacgttcct
    52921 tataattctt ggtggaattt aatacttctg tttccggcaa caatgtcccg gaaatgattt
    52981 tcaaatagtt aaacctgccg gcatactggt caatatatgt tttccacaca acagcagcaa
    53041 atggtgcatc aggttttgat gtgataacta ctttttcttc tttattattg ggattcaggc
    53101 cttcaacctg tttgtttaac tcccacgcag gaacataatt tttaataacg ttaagtaaat
    53161 tctttatacc aatggctttc agtgaagaac cgcatataac cgggaaaacc tctgctctgg
    53221 caacaagagc tttcaagcct tttttcattt catcttcagt taacggttca ccttctaaat
    53281 acttttctat gagtgcatca tcgccttcag ccgatagttc catcaaacta ttgcgtgcat
    53341 cttctgcagc ttttttcaaa tctgccggta tatcgcttac ctgaattttg ccatcaggtt
    53401 ttggcatcat agctttcatt tcaataaggt ccacaatgcc actgtgttta tcagcttcac
    53461 caacaggaat gcaaacagct gcaaatttgg cattaaaatg ggatttcaga ttatcaataa
    53521 ttgcattaaa atttgcccgc tctttatcca tcttatttat aaaaatgatt cgggggatct
    53581 tttttgattc taaataacgc catacttttt cagtttctat ctgtacacca tcaactgcat
    53641 caaccactac gatagctgct tcagccacct gaattgcagc tcgtgcttca ccaacaaaat
    53701 ctgacatacc aggtgtatca attatattta ttttaacatc atctatctct acaaaaccca
    53761 gtgcactgcg tattgatatt ttcctgcttt tttcttcctc atcataatcc gatgtcagcg
    53821 acccatcatc cggcttgcca attttatcaa tttgaccacc aataaacaac attgcgtcaa
    53881 ataatgtaga ttttccggta cttccatgac ccacaatggc aacagttctg acattttcag
    53941 tagaatattc ttttagcatc ataccctcca gcgtacttat tcactaaatt aaaatgagat
    54001 attctctttt aggacgaaaa gacaaaagct tttgcagaaa agtaaaatgg tatatgtttt
    54061 ttgtaaagta ttttatttta taaaattgaa agcagtgata cagttattct tttttataca
    54121 atacattgat atttaccata gtatatgaat atggttatac atattttatt cttgattatt
    54181 ccggtaatag tatgtagttt gcaccaaaaa gcatcaggta tagataacat tatctaattt
    54241 ctggatacag tatttatatt tgataacata ttcttattac aatttcatat tgtaaaatat
    54301 tcatgagtta cataaaaaat atccataaca ttatgagagc attacaccat tataccatcc
    54361 cttttatcgt tttactttgt attcacctgt ttgcagtgaa tactggtagt tttgcacagt
    54421 atgtctatga agcatccaca ctgcataatg ctgcctcgcg aagccagact gtacagcaga
    54481 ttttttacca gcgctgggat accatattca gtaactatct gcactttgat gcatatatac
    54541 acctcaactg ggtatatgat ttttatgtta actcatacaa tcgtggtacc ggtaccactc
    54601 aggctgttcc catgcgtctg gtcaggacat tctcggatat agcattgtta tttcccgttt
    54661 atacaccagt taccgaaaca ccagatgaag ctcagccagt tgatataacc gatgtatcaa
    54721 cattccgcaa gccatgggtg gacaattcaa ttttgcttgg atttacagcc tgtggatatc
    54781 actatggctt aacccgtact gttgatatta accgtggaag tgccggaaca gagtcagcat
    54841 ctgattatgc cttttcacaa ttttttgacg acattgttgc aatcacagca gtgtttgttc
    54901 cctatgttac gcttcatgca ggaattgtct ttaaccagca gatagagccc aatgaagatg
    54961 gcacgctgga ttataatttt gacccatcaa caattaccaa acgctacttc gcatcagtaa
    55021 gtctttataa catactaacc tggcgctcaa cggctgcaaa agaccgtctt gagtcctacg
    55081 atatcagcct gaacataaca caatttttat ccattacaac aactatacct tcatacctgc
    55141 cgcagataac aattggctat aaaaaatatc aaaagtacaa tgacgagcca tatgacccgg
    55201 tatgggtaag cactgcaagc ggcaaatcaa ataccatgag cgatagttta aagcaacatg
    55261 caacactgta tacctattac acaacgttat ccaaaaactt tggtaatttt ttccttgcca
    55321 caagcattga gcttcaacat gtagatagaa caattgttga taaacgaaca gaacaaaaag
    55381 tatcactgcc tgtaatgcgg ctatggtggg ctaaagccgg atatgatctt atggatagac
    55441 ctggttggga tttcatagta tggcttggag taaacaagtt ttatgaccct gctatgagct
    55501 atcacagtaa gcacagcgat aatgatacag ggggatggtt tgctatgatt gatggcacat
    55561 caccatatat tacgttgtcc atcaaagctt catataatga ttacagtgag cttgataagc
    55621 tgatagaagc tattgacaag tatatcattg aaggaagtgc atcgttcagg atataatact
    55681 atgccaaaca aatttataca aatattatta ttgtgggcga tagttactgc caatgtagcc
    55741 tgttcctacg tagcagatta tattgaagga gagattacca accgcgcccg tttttccatc
    55801 agtgcccact atgaagctgg taccggagtc attgttgagt gggatgctaa tcctggcaat
    55861 gactcatttg ctggttatga aatttacatg acccaggaac caaacaatga atattccgat
    55921 tattttgtta ttggtgcacc ttatgacatt ggaacttcat catattttca aattgatggg
    55981 gcgctatcaa attcttcaac tacacaattt attcatactt ctttaccacc atctggcatt
    56041 tatttctacc gggttggcgt cattgaatgg gatgaacgcg actataataa tgatggcgaa
    56101 gatgaaaaaa aaccagcttc acctacccct gcattatata aattatatac aaatatagct
    56161 gaaatcagtg gcagtgcgat ggtggaaatt ccataattac atggctctga cccttttgtt
    56221 ctttacaccc agcctgcaat agaatatcgt ttgcgatatg cctcatcata gcgcaaaaag
    56281 cgtgctaccc tttgggcaaa actatccccc agttgcataa agtactcatg atggtctata
    56341 ggtactccta catcggcatg ctcatctaag tatgcctgta tgctgctgta gcgatattgg
    56401 gtggggtcag agcataaatt cttcctcacc ggattatacg ctaaatacca taacaaccac
    56461 agcaaatagt gaaacccatc cctggcaaac tctatgatgc tatccttata ccgctcattc
    56521 cagaacggac ccgttcttcc gattatctta ttatacattt ccgcaaaccg tgccttaata
    56581 tactgaacaa tacgggaaat gggagcacca tcctcaaggg tatgaacgac cagatggatg
    56641 tggttatcca taatgcagta ggctatcagc ttaaaccgat atttttcttt tgccttgcgt
    56701 aaaatttcca caaaacaggc tttgaaatac tcttcttcta acatctttcg ccactcaatg
    56761 cagcggcttg tgatgtgata ggtaagaccg ggcatttgta cacgcaattt ccgtgccata
    56821 agtattcctc catacactga tgtactatga gagacgtatg tttctctcac tctatacaac
    56881 gcaggtggtg aaaaaaatta acgggatttt gggaaaattt ttatttttta gaaaaaaaat
    56941 taaaaaacat agggtcagag catgataact aataggaaga ggaggatata aaaggattat
    57001 tactgtgatg acaaatcatt aaatatcctg tatatcctat acatcctgtt aaaaaaaagg
    57061 ggtcagagca tagtaacgga taataacagg atgcgaaggg aaaaaaagga ttagctatag
    57121 taaaaatata aatccttaac atcctgtata tcctgttaca aacatagggt cagagcttaa
    57181 ttaaatgcac catctttttt ttacagggat tggtgttaaa tacataaaaa ttgaattaat
    57241 aaagctcatt aaacatctct agcagtagca tatagtattt gtttttataa aataatcaca
    57301 tcaaaaacga gccatgaata aatcaatatt taaattttta tatttaaaat aattacaata
    57361 ttataattga cagaaaagca ttctcattcc attattattt ttatctattt gcaggaggaa
    57421 atccatgcat tactgtatta ttggcaatgg cgttgcaggt accgaagcag cgctggcaat
    57481 tcgttcccag gatagttcat cagcaattac cattattacc caatcagctt atcatatgta
    57541 ctatcgccca cgccttattg aatacctggc cggcgatatc aaaccagaaa atctttatat
    57601 ttacaaaccc gcaacctatg atgaaaaaca cattgaagtt ttgttcaaaa ctaaggttat
    57661 tgatattgat accaaaaata aatatataac gttacatcac ggatcaaaga ttaaatacga
    57721 taaactctgt attgccaccg gtgccactcc ttctatgcca tcaatacccg gaaccacgtt
    57781 gcagggtgtt ttcagcgtta gaactattga tgatgcagat gctattatta accacataaa
    57841 taatacacca gacaaaaagg tagcagtaat tggtggtggc cttctgggcc tggaaactgc
    57901 atacagttta tccaggcgtg ggtgcagcgt tacggttatt gaattttttg acaggctgct
    57961 gccgcgacag ctggatactg atggagcagc aattgtcaaa aagctccttg aacaaaaggg
    58021 gttacagttt gtactgggtg attctgtcac ggaaatagta ggcgatagtt ccgcacaaaa
    58081 agtcatatgc aaatcgggaa aacaacttga agcaagtact gtgatattct caatgggaat
    58141 taaaccggag atttctcttg caaaaaatgc agggattgtt gtaaaccgtg gaatcattat
    58201 taataattat ttgcaaacgt ctgaacctga catttttgcc gctggtgacc caaccgaata
    58261 caatgggata tgctacggca tatggccagc cgcacgtgaa cagggtaagc tggctggatt
    58321 aaatatggct ggtgccaggc aggaatacaa aggaacattg ctttcagtgc ttttaaaaat
    58381 aacgggcatt gacctgtact cagcgggtga ttttaataaa gaaggcacca aatccgtagt
    58441 tcataaaacc gatagtatat atgtaaaatt tttatataat gatgttgatc ctgttgcagc
    58501 aattgtcatt ggcgatatga atatcataaa acttgcccgc aatgtcatgg agaaaaaagc
    58561 cgaaataaaa gaacttgtta agctgtttgt ttaaatattt tatttacagc aaatatacta
    58621 tttgctataa ttagctttaa gatttacata aggtttccaa catgaaaaca ataaaaaaac
    58681 atcaatacat cattgataca aagggtaaaa aaaatgctgt aattttacct ttagatgaat
    58741 atgaaaaatt attagaagat ttacacgatt tacgaataat tgctgaaaga aaaaatgaaa
    58801 aaaatataga ttttgataca ataaaaagcc ggttgaagta atcttgtgta cactattgtt
    58861 tttaaaaaat cagctgagaa agatctatct ttcataaatt ataaaatcat taagagaatt
    58921 ataaataaag ttgaaaactt aaaaaataat ccatttccac aaaacagtac aaaacttatt
    58981 ggaacagaat caacatatag attacgagtt ggtgattatc gtatcatata tcaaattgat
    59041 gaagcaaata agattataac aatttactac atccgtcacc gaaaagatgt atatagaaaa
    59101 ttaaagtaat atcaatattg atcatatttt acttcccctt taaatacttt tctattcggt
    59161 ttaatccttc ttcaatgttt tcaagcgatg ttgcatatga aaatctcaag aaaccctgac
    59221 cagcacttcc aaaatcaata ccaggagtta ctcctacttt aaccttttct attatattga
    59281 acgcctcctt gtatgaatca ctgcagaatc ctcgtgcatc agcaaataca tagaatgccc
    59341 ctgttggttc cacatgaatt ctaaatccta ttgattgtaa ccgctcaatc atatattggc
    59401 gccgggtgtt gtatgtttga cgcatggttt caacatcctg ctgtgcatat tttaatgcag
    59461 caatgccagc ccattgaata aagttatttg ccgaaatcat aaaattttgg tgaatccgct
    59521 gcattacttt tgaaaattct tttgggaaaa tgcaataccc taaccgccat ccagtcattg
    59581 cgtatagctt tgaaaagccg tttataacaa aggctttatc agtaaactcc agtatagaat
    59641 gcgctctgtc tttatacaca aggccatgat aaatttcatc tgatataata aactgtttat
    59701 caaactgtgc tattgtttgc agtgcgtctt tgctcatgac aattcctgtt ggattgcagg
    59761 gtgaatttat cataatagct ttagtttttg gagttatctt tttctttata tcatcaggtc
    59821 ggtactgaaa accctcttcc ggataggtaa cgatttcatt gaccaatcct ccggctgcaa
    59881 tgataaaatt ttgataacag ggatagcggg gatttgacac aataacatct tcaccggtat
    59941 tgatgatagc aagcatggca aaaagaagtg ccggcgaagt cccggttgta accagtatct
    60001 gatcaggtga cagcgacaca tgatattcat tgtaataata ttcacagatt gcttcacgca
    60061 attcaagtaa tcccaacgca tgggtatatt ttattttttg ttttttcaat gcctctatgg
    60121 ctgcttcttc tatcacttta ggtgtcatga aatcaggctc cccaacctct aagtgtataa
    60181 catgctcacc tttgcgttcc agttcttcag cttttgccat gacatccatc acaatgaatg
    60241 atgtaaattc atttgcacgt tgcgatagca ttgtgtgata gctcatatca atatactctc
    60301 actgtaacat aaaattttgt gtatagttca tagtgtatac tattattgaa gatagcctac
    60361 tgcaagcaat ctattactta catcgtgacg ctacaagaaa accatcaaat cagtatcatg
    60421 gcaagtctaa tttacagtaa ctatcgcaaa aaaataatag caactattga caaaattata
    60481 ctttgacttt attttaatca ttgatttatt aacagtaact atcctgcaca ctacaataag
    60541 gagtgagctc tatggttcag attttaatat tatactactc ccgttcaggc aatactaaag
    60601 aacttgcaaa ggcaatagca gaaggcgtta aacaggtaga gggtgctcaa tacatcctgc
    60661 gctcaacaga agaagtaacc aaagatgatt ttttgaattc acatggtata attgcaggct
    60721 caccagtata ttttggtaca atggcagcag aattaaaaca cctttttgat gattatgtta
    60781 ttattcgaag aaagatggaa aacaaggttg gcgctgcatt tgccacttca agtgatccgt
    60841 caggtggcaa agaaacaaca ctcctttcaa tattacaggc aatgctcatc tacgggatgg
    60901 ttattgttgg cgatcctatg gaagcaacag gccattatgg tgtatcctgc acaggtgctc
    60961 ccgatgaaaa gacaaagaag aatgcagctc tactgggtaa gcgtgttgcc gaattagcaa
    61021 aaaagctctg ggattaaata gtgaaacatt atgccatcat agtagcaggt ggtatgggac
    61081 agcgtatggg aactgctgtc cctaaacaat ttttacagct tggtaaaaaa ccaatcattg
    61141 catggtcaat tgaaacattt gctgcatcac cgttaattga taaaataata atagtcattc
    61201 atcccgatta caagcaacag ctggaagatg ttatacacac ctatttccct gataccgaca
    61261 ttatcatcac acacggtggg caaactcgtc aggaatcctg ctttaatgga ttgcaagcat
    61321 acgatttttc caatgatgat attgttctga ttcatgatgc agcacgcccc tttataactg
    61381 atgacatgat taaacagtgc attgagatag ctgccttata tggtgcatgt ggtatatatg
    61441 tttctgtgaa agaaacaatt gcacaggtca atgattctac agtaacacac attttaccgc
    61501 gagaaaccct ttacgctgca caaacaccac aatgctttcg ttattcaata attatggatg
    61561 cacaccaaaa agcacgtgaa cataacacga ctaatgctac tgatgatgtg tcattagctt
    61621 tacttgccgg gtatacagtt aaagccataa caggagattt ttatacaaac ataaagataa
    61681 ctacaaacga agatatggag ttagctaaat tgttaataaa agaataaggt atgcacatca
    61741 tgtcttgtta gtataattga gatcattgaa gaaattgttt attaaagttg gttataaata
    61801 taacaacttc ttattgaaaa gcattggggg ttgcaaacca atttaagatg ttaattcaaa
    61861 ggaatttgat caaatgtaat tattgtaccc acattggcct gtcaggtgct ctaacgctat
    61921 actcaatatc gtctcaaact agaggacatt aagtgcaatt ctgcttgaaa tagttatacg
    61981 tggtttattg agtattcagt atgattggag gataaagcat ggaattcata tataatagga
    62041 catgttttgg cagacattat tgattattct aaaatattag atttgaaaaa attattatgt
    62101 aatctaaaat tctaaacttg gaaaattaat tactaattat atttaatgaa aagatgataa
    62161 atatacttga taagtataac aaaccgattt tcttttatgg attatcgttg ataattccat
    62221 gggtactatg gttcatcgtt gcctatttta gtcatctgga aaatcaagga cctatcgttt
    62281 ttttgattcc ttcattgggt atactcgggt taatatcgcc tgttattgtc gcaggctact
    62341 tattccatag tgacagagag ctcatggaag atttgaaaag caggattttt aatctacaaa
    62401 aatttaatct attctatttt gcgataacat ttttcttaat tccactttca attgttatag
    62461 ctcaattgat ttctgttctt tttggtcaca gcctttccca gttttatatt tcaggtaaac
    62521 catcatttaa ttcttttatt ttttcaccgt ggtttatact tatatttgca ccaatagttg
    62581 aagagttagc ctggcattct tatggaactg atactttaag aaggaagttt aacctgttta
    62641 atacatctat aatttttgcc atatactggg tattctggca ttttccactg gcttttatta
    62701 aaggctatta ccatagcaat gttgttgcag aaggattatt atattcattt aattttattg
    62761 caagtatttt tgtgtttgta cttttaatga actggctata ttataaagca aaaagaaata
    62821 tatttgtagc gactattttt catctcattg caaatgtatc aaatgaaata tttgccacac
    62881 acccggacag caaagtaata cagactgtat tattgttgat tattacaata atccttctgg
    62941 ttaaagatag agagatgttt tttaacaggg caataattga gtagaaaggt acaatcatat
    63001 gatgaaaaca attgagaatt tattaaagaa ttcaggactg ccaggaccaa ggggtaattt
    63061 agaactttta tattcttttt cagaaaatgc cacaaaaaat gaagtagaag aatgtttatc
    63121 cttctataaa gatgatttgc ataattctcc agaagaattt gttgtaatgt gtggtatagt
    63181 tggatattgc attttaaaca aaaatgatat taaaaagact ttagcgacga taagaaaata
    63241 tgcatctcat agtagctgga gaattagaga agcagttgct attggtatac aggaaattac
    63301 agaacacaat acaagcgaga taattaataa tcttgaaaac tggacggatg gcaatgactt
    63361 agagaagcga gctgttgttg cagctttatg tgagccaaaa cttttaaagg aaaattcatt
    63421 tgttattaag ttatttgaaa ttctttataa gattacaatg agctttgata aaatcaatgg
    63481 aaaactttct gaaagccaaa attctttaag aaaaactctt ggatatggat ggagtgttgc
    63541 tattgtttct atgccagacc agggaaaatt agctttcgag aaaatcgcaa gatgtaataa
    63601 taaacatatt aaatggattg tgaaagaaaa tttgaaaaag aagagattaa ctgtaatgga
    63661 taaaaaatgg gttaataagt tgcgtgctaa ttttgtttaa taaaggctat acggcattaa
    63721 aacggcgatg gataaggaat gttaaatgaa attctgtccg aagcctttta aagcagtata
    63781 cctaaagtta ccaccagaga tatagcagag caacagataa aaacttcttt ctccacaaaa
    63841 tgtcccacta ttgctttgaa gctcattttg tttgacatct ttttgggggg cgcagtatac
    63901 ttataaagtc tggaaataac acgaaatttc gtatatttta ctgaagcaga aagtatgatg
    63961 aaatacggcg tatagaataa gatgagtaaa atgtcgcttt gctccatttt gcacaactcg
    64021 acagcgggca agcccgccac cgaaggttag acgatatttt cgcaaaaata aagaggtgag
    64081 ttatggatat atggaaattt ttgacatcac acataaagaa cacattattt gtaaccctat
    64141 gagtgaagac aaactaaata aattgataaa tttgttaaaa ctgaacgaaa aatccaatgt
    64201 attagaaatt gcttgtggta aggctgaatt tcttattaga cttgcagaaa catatgatat
    64261 ataaggaatc ggaatagata tttcaccatt ttgtatagct aaagtaaagg aaaagcttaa
    64321 aaatcgttgt cctaaagcaa aaataaagtt tattgaaatg aatggagctg attataaaca
    64381 ttcggagaaa aattattttg atctagctat gtgtattggt ggggaatgga tttttaatgg
    64441 attcaaaaac accttgagct atttgcttca aaatgtgaaa caggatggaa tgattatagc
    64501 aggatgcccg ttttggcaaa aagaaccatc agaagagtat ttgaaaacag ttgaattgaa
    64561 gaaagataat tatggaactc attacgaaaa tatgctaatt ggaaaagaat taggattaga
    64621 attaatttat tcaatagtta gcagcaagga tgattgggat aattacgaag ggttaaattg
    64681 gtatgcaagt tttaaatata taaatgctaa tccttttgat aaggataatc aagaattata
    64741 taatagaata ataaaacagc aagatagtta tttgaaatgg ggcagagaaa cgataggatg
    64801 ggcaatatat atctttcaaa aggtgaagat ataaatataa tattacaaaa acatcgtcta
    64861 acagcaagtt gcaggttccg gcgagttcgc ctacactcaa attgctcgtg agtaccctcg
    64921 caaattctgc aacttgtgcc gcgttatcta ccattgccat atagaaagag gattttatgg
    64981 acatcaaaac atttttaata ctaggtggat atggaaatgc tggtcgtgtt ttggcacagc
    65041 tattattgca agaaacaaat gtcaggttgg tgttggccgg tcgaaatctt gataaagcta
    65101 aggcagccgc tgaccaattc aacaatcttt ttgaaggaaa cagagtcaca ggagtgtttg
    65161 ctgatgcgtc agacttagga aagcttagac agacattcaa aggtattgat tttgtggttg
    65221 tagcttccag cacggctaaa tacacaaaag agattgcaag aacagcattg gaagcaggtt
    65281 gtgattatat ggatattcac tttggaccta aaatctatgc cacattgtat tccttagtcg
    65341 aagccattaa aaatgctgat cggtgtttta tcacaggagg cggatttcat cccggattac
    65401 ctgccgcttt aatacgctat gctggtcaat acttcgacag tatggagaag gctattgtcg
    65461 gtggtgttat gaatataaat ttcaaagagt atgaattttc tgaatcaaca aaaattgaat
    65521 ttgttgagga ggtaatagat tttctaccat ttatctacaa aaatggagaa tggcgaaaaa
    65581 taaatatcat gagcacaaaa gattatgttg caatggattt tggaagtgaa tttggcaaaa
    65641 gacaatgtgc ccctatgttc tttgaggaac ttcgtgaaat accacttatc tttccaactc
    65701 ttaaccagac tggtttttat atagctggtt ttaactggtt tacagattac tttgtttttc
    65761 cattcatctt tatttctcta aaactatttc ccaaaataat aatcaagcca atgtcacaac
    65821 tgatgcagtg gagcctttca actttctcaa aacccccgta tggtttagtt gtgaaattgg
    65881 aagccattgg tgaaaaaaac ggcaaattaa aaaacattga aatacaactt tcacacaaag
    65941 atggttacta ttttactgct attcctgttg tggcttgttt gctgcaatac cttgatggat
    66001 cgattagaaa gcctggtcta tggctacagg ccaacattgt agagccaaac cggtttatgc
    66061 atgatatgaa acgaatggga atcgatgtta aagttcaaga caagaatggc aacggcagat
    66121 aacagcgtgt ttgcggttcg cttcgctcac ccaaatgcct tttggcactt cacaaaccag
    66181 ccgcccataa ggcaaaatgt caggtcaggc cagcccattg tgaacaggat ctgatgaatg
    66241 taacttaaaa tagaaaaaaa ataaggagag aaactaagat gaaaagatct caggttgcca
    66301 tagcaaagtt tacaaaggac tacaattgtg ctcaatctgt tctattttct ttttgtgatt
    66361 atttaaattg tgataaagat gttgcattga aaatatcatg tggctttggt gctggaatgg
    66421 gtcgtaagga ggaagtctgt ggggctgtta ctggtggaat aatggtaatt ggattgcgat
    66481 atggaagagg agaaaaagga gatagctcat ttactgaaga aacatataaa aaaacacgtg
    66541 aattcatgga caaatttaaa gaaaaacatg gaacgtttat ttgtagaaag ttacttggag
    66601 gatgtgaatt aacaacaccc gaggggcagg cagagtttaa ggaaaaggat ttatataata
    66661 aagtatgcaa gcaatgtgta agcgatgcag ttgatattct ggaaaaagta atctaaaaag
    66721 tgtagtccca tcctccttgc caatgtgaaa tgtatggcac tcgaaaagca gggtttaagc
    66781 ccaggcaact tctgtaaact gcgaatatta tgtttaataa tgaaaactaa gtagataata
    66841 aaaacattgg gaatattatg aatgaattag aattacaact accaaccctt gagtacaaaa
    66901 agtcagcaga agatttcaaa ggtgaatttt ttgaaaatca ggagactgtt atcaatggaa
    66961 gtgcattgtt tgaccagatg gaatatgagc aatggttgat acataataca aataatcaag
    67021 aaggaaatat tgttaataat aattgggtgc cagcaactac attttttgct gtcagaaaaa
    67081 atgacctaaa aattgtgggg atgattgata ttcggcacaa cttaaaaaat gattttttag
    67141 cacaatacgg aggtcatata ggcttctctg tctgacccag tgaaagaaaa aagggctatg
    67201 gaacagaaat attgaaaatg ggaattgaat atgctaaatc cttaggtatt agaaagttga
    67261 tgttaggttg tttttcggac aatctaccat ctattaaagc aatagaaaaa tgtggaggta
    67321 aattgacaga aacaaaaata ttttcagaca atcaactttt aataatttct gatgcgaaaa
    67381 ataaaattgt aaatatttat tggattgact tatgaattct tatcaaaaaa tgagtacttg
    67441 cacacaataa ttggcatatg ttattaaaaa ggcgcatagc cttggcctga tgttgcaaaa
    67501 ttgcccctca attttgaata tcggacggcg tgctaccgcc ctcgatgtga ttgttgcaaa
    67561 gcaatttccc acatctttgg ctatccgcaa tgtctgccca gtccactgcg aatacccacc
    67621 gcctgttata cgacatattt ttttcagcgg gtgaaagcgt acatctgagc aggattcagg
    67681 aaaataaatt attatatggt aaaagatgaa ataatctcca gttcaattca aaagtgcaac
    67741 taaagaagta aaaaagtaga ttaatttaat tttaatctat ctttatcttt ttgatatgac
    67801 ccagttgttt tatttcatca aaaagcaacc gaatactgaa atcttcgtcc atgcggcaat
    67861 gtacaatcaa atccgatgta tgattttcca catcttcagt gatggatacc tgccgtatgt
    67921 caatatcacc agcatgtgtc tttactgctg ctaaaatctg ttttgctgca atagcgtcag
    67981 aataataggt tatctgcaat attctgattt tcttctgttc aacaagccat agttcaactt
    68041 tttcaaatac ctgtaatgtt atcaccaata gcactgtagc tatcgcccct aaaaaataaa
    68101 accctgcacc aaataccata ccaatgcctg atgttgtcca tattgttgca gccgtggtaa
    68161 gccctctgat gctgaatcca tatttaaaaa ttgcacctgc acccagaaat ccaatgccgc
    68221 ttataacctg cgcagaaagt cgtgcaggat cgcccgaacc tggtgaatat acatacggaa
    68281 taaaatatga caaaatcata atgatgcatg cacccaacga taaaaccatg tgcgtgcgaa
    68341 gaccagcagg ttgatagtga cgctcgcgtt caaacccaat acagaaccct gcaataattg
    68401 cacacataat ccgtacaagc acggtaacag gatgcaacac atcaagaccg gtaacagtat
    68461 caattactga attcatgcaa ttgttctttc aataatttta tttggttttc aatgctctca
    68521 agtgaagtgc ttccgtatga tttttttcgt tctgttgatt gttcaggatc aaggtattca
    68581 taaatgtcaa aatcaaattt ttctgaaaat ttcttcaatt cttctaaggg caaagtaaaa
    68641 aagtctttat tgttctgttc acaatactta actaaagaac caacaatctc atgcgcctgc
    68701 ctgaaagcaa tacccttcat aacaagataa tcagcaacat cggttgcagt ggaaaaattt
    68761 ttatatagtg ctttctttgt tatatcttca ttcactgtca aatgttcaat catctcagcc
    68821 atgcctttga gtaccagctt cacggtatca atggaatcaa acagcggttc tttatcttcc
    68881 tgtaaatcac gattataggt cattggcaat cctttcagca ctgtcaataa tgaaattaaa
    68941 ttaccataca gcctgcccgt cttcccgcgt ataagttcag caatatcagg attacgcttc
    69001 tggggcataa ttgatgatcc cgttgtaacg ctgtcagata gctttataaa attaaactcg
    69061 gccgttgacc acacaatcaa atcttccgct aagcgtgaag aatgcatgcc caccattgca
    69121 gcaaagtaca aaaagtctgc aataaaatcc ctgtcactaa ccgcatccat tgaattgtgt
    69181 gcgatagttc caaagccaag ttccttggca atataatgcc tgtcagtggt atagttaact
    69241 cctgccagtg ctccgctccc aagagaaagg atatctgtag cattgaatgc attgtgaagc
    69301 cgtttgagat cgcgcagata cgcccatgca tacacaagaa gatgatggct gaagcgaaca
    69361 ggctgtgcta tctgcatgtg cgtatatccc ggcataacga tatgtttatt ttcttgcgct
    69421 ttctttacca gtttctgtat cagatcaaca cagagttttt gtatatcatt aatttcgtct
    69481 tttaaataca aacgtacatc agtgataacc tgatcatttc gtgaacgtgc tgtatgcaat
    69541 ctttttccag catcgccagt aagctccgtt aatcgggcct caatattcat atgaatgtct
    69601 tcaagctttt cgttaaacgg gaatgtacct tcttcaattt ctttctgtat tgtttgtaaa
    69661 ccttttataa tagcattgca atcatcctgg gtgagtatac cctgttttgc cagcatcttt
    69721 gcatgcgcaa tagaaccctg tatatcatgc ttgtataacc tgacatcaaa atgtaatgat
    69781 gcggatatct gttcagttat tggtgatgtt gtatcactaa accttccacc ccatagcttt
    69841 ttgtttttca aagtggttct cctgtaaaaa atagagttcg ccccaactca tcgaggggct
    69901 ggagcgcata cttcattttt cataaaataa tttgaaggaa ttattgctga ttattaccaa
    69961 taatgtattg atgtcaagta taaaggagaa tctacgattt tatattctga agtattatgt
    70021 caagataatt gtctaattcc ccaatcagag gctttaattc atattcaagt atatctgaaa
    70081 gactgactat atcattgttt tccattatac caacaatttc cgagagcata tcctgcaatc
    70141 ggatattcag ttcttcaaga gattgatcat tatggacaat atctatgaga ttaatgccaa
    70201 ataccggtga tacctgatag caggttcgca tatataaata aagaaaatct accagtgtgt
    70261 gtattatttc cagcgcttca ttatctttac ctttctggta cagaccagct atcgcctcta
    70321 aattatctac ctgctttgaa accattgctt ttaaatcaag cagtgtttta aaaagaacat
    70381 caggtgaatc cacactctgt atcacaagat gcttcagtgc atcactgcaa agtaataccc
    70441 tgaataatgt tgttacatca tgaaacaatt catgaatttt attaatatca ctattggtcc
    70501 tgttatcttt tatcatgtgt tgtaatgatt taattgaatc aatatattgt tgtactgtca
    70561 tatctttaaa acgtatgttt tccctttcca gaccaagcat atgcaatacc gactgtaata
    70621 ctgaaacaat ccattcaata ccattgttta actgtgtaaa ctcagtatca gttaattgtt
    70681 ctaaacttcc tttatctttt aaatattgct cagctctgtt acaatatgca ataccttcct
    70741 tgattgaatc gatcacaaca tcacctacag actggacata gcaattaata tttgtaatat
    70801 catgtattgg cgtatcaggt gccgaatcag gctggtacat cacaccatca acaattgctc
    70861 ccgaaaatat cagacttcgt tgagatgccc agttgcgtac cgagttcatc acatctttaa
    70921 cagtactctc tttttctatg gtgaaatcaa caggatgatc attaattaac aattgcatgg
    70981 tacacctctt acacctttat tttatttttc agtttgtttt tgcaaatttt tcatttgcat
    71041 atcaagccaa ctactctgcg acttggatcc agaaatagcc ttttccatag cggcaaattt
    71101 ttgtcttaac ttttgttcat acgctttcag atgttcctgc ttacgttcga tacgctcatc
    71161 agtggcttta atagatgcat cttcaagatc catttttgcc actataatgt ttttccctgc
    71221 actaacatat ggatcaagga tactttctat tttaaaagct aatccattat ctatacgatt
    71281 gtctccatca ttatccgaac caaaaaaatt ctgtacacct tccggatttt ctgacaacac
    71341 ttcaaccagc tttgcttcat ctattataag ctttccctct cgtatagttt cccactttgc
    71401 atttatttcg ccagttgaaa cacctatttg atacaacatt ttaataggct tttctacatt
    71461 tgaaggatag gcagaactaa ttgcagtctt taatgcattt tgcaacctta gtaaagtcat
    71521 atcgcccata aaaatgccgt ttttattttt atccttttga taatcaccca ctttatcgct
    71581 ttttacagct tttgttaatt cattattaaa tgtaagataa tcattatatg cttcaacaaa
    71641 tttctttatc ttttcaactg ccgtttcagt attctgccga attgtgatag taacgggttt
    71701 agttgaagta cttaacaaat taaaggttac tccctttaat acatcatcaa ttccattatt
    71761 ttttgatctt actatctcaa ccccatcaag ttttattctt gcatcatttg cttcagcaat
    71821 aacatttttt ggatcaagta ttccttcgcc ctttatttca gtaaccagtg aagcatcctg
    71881 aaaagtagct ttgccatcat tacaatagaa caaaattttt gttatctttt tatctgcaaa
    71941 atcctgacct ataggtattt cctgtttccc ctgtttattg tgatctagct tatatatttt
    72001 ttcagtacgt ttcccatctt caaccgaaac cacaccaaca ccaataatat cttcaacttc
    72061 tttttttggt ttcttttcaa gaggacgttc ccgggaaata ttataccctt ttaattcaat
    72121 accttttatt actgttgttt cttcaggacc tatttcaatt ttatatggta ttacctcttc
    72181 ttctgatttt tcttttgaat atgtaacatt taactcaagt attgattgtt tttgtgcttt
    72241 atactcaacc ggtaaaatat attcacgcca taattttgca tctattgtta tcttcttccc
    72301 atccttatca actgtcaaag ttccatcttc atcctccaca ggtttatccc cagtataccc
    72361 ggtaaaatat tttgtatcaa agattattcg ctgggattcc ttagctgcgc ctttttcacc
    72421 tttaacaagg ccaatctttt ttaaatactc ttcatcacct cgtatcttta tttccccttt
    72481 ttttcccgga acttttgatt caatggttaa aatgctttta tcatcgatag ttttaattgt
    72541 tgctgccgaa acaatacttg atgcaatttc attaactttg tcacttaaag ttctgagatt
    72601 accaccacga aatttaatag tatatgaatt tccatcaact tcaatgctga aggttcctgc
    72661 aggcaatatt tcatcttctt taatctgatc agtggatact ttgtgggttg aagctaaatt
    72721 caatacttca agagtatgtg tgccgttcac agcataccgt gacgcctgag cctgcaaaac
    72781 actggtatca gaaacctgta cacctttttc cctgaatggt gacctgaaac cgtataaatc
    72841 tttcgttgca ttatttaaag tgttcagctt tgtttgtaac tggcgcaaag cttctttccg
    72901 gcgggagtag gtatcttttt cttcctgcca ctgtataata ggcctggctt ctacctcaac
    72961 cagcttttga attatctggt cggtatccat ccctgatgct acgccgccta aagtgactgg
    73021 catacatgta tcctttcatg taagataata gtaaagatag caatgtaccc atactatttc
    73081 aatacattat cggcaaaatt caccaatcac taaagcgaca tattacaata annnnnnnnn
    73141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    73201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctgcccttt gtcgattcag cttatctaaa
    73261 gctgcatcga tataccctat catggcgttt gcctgaccaa cagtggaaat tgaccgtttc
    73321 cggttaccat cataaaggtt taaagccttg gcactcatcg tagcaatata ggctctaatc
    73381 ctttgaccct ggtttggtcc aatatggaag aatatgcttc ccactcgcga attacgcgca
    73441 taaatgcctg tgagcatctt catacggttg aactcagccg atgttgaaat cctgtcgatt
    73501 tcatcaataa gctgcgaaac ttcaacctga atctgcgcac gatcggcatt tgaataaatt
    73561 ccattggcag cctgtaccga tagcatccgg attctttgca gaatatcatt aatctgctgt
    73621 aatgatcctt cggcaacctg tataaacgaa agcccatttt cggcatttcg ttcagcctgg
    73681 aacaaaccat taatctgtgt cctcattttc tctgacacag caagccccga agcatcatcg
    73741 ccagctctgt taatctgctg accagatgcc aaccgctcaa tggacttatc cattcgcgat
    73801 gagacgatct taagctgcct gttagcgaat atagcgctta agttgtgatt gattctcata
    73861 aactctccct cccttgaacg tttacaggat atcccagtat ttctgttgag atatctcagg
    73921 atttttcagt atgttcatca tgaacataca gggtctaatg ttagaccagg ggttttctcc
    73981 ccaatgttgc ccggactgcc catcatccag acctcatcag ctgccgcatt tccggcagcc
    74041 ggtgacgccc tcctatcatc accggccgcc agaacaagca gccgaataga aaggacagcc
    74101 ccggtacaat cgtccaggga gagtgagaat catcacaaaa gttttcaaag aaccattagg
    74161 tgtgtcccct agctgattac acaagtaatc gtaacaccga ttggggttgt aaattagcct
    74221 gtgctaacat tgcagtgcct gactgcacaa ggactgtgtt cttggtaaaa tctaccattt
    74281 cttcagccat atcagtatcc cgaatacggg attctgatgc ctgaacattc tcatacgcgg
    74341 tcataagccc ctttgcagta tgctctaatc tgttataata ggcacctaag tcagcacgct
    74401 gcttggaaag cttctgcaat gcggcatcca ttacaccaat ggttgcattt gcaccacctg
    74461 gtgatgtcag tgtaatagct actttaccgg tagcatcttt catcctgaaa gcctgagctg
    74521 tcatggtccc aatgaacacc cttacgcgct gatttgcgtt aggtcccata tgaaaccaca
    74581 tgcttgactt tggattaatc ttggagaatt caccggttaa tatcttgaac cggttaaact
    74641 ctgcctgcga tgctatacgg tccacctcat ctaccagggc agatacttca acctgaataa
    74701 gttgacgatc tgcattggta tagataccgt ttgcagcctg aaccgccaga acccttattc
    74761 tctggataat ctgggcagtc tgtgctaaat acccttctgc agtctgaaca aacgacatac
    74821 catcttcggt atttcgttca gcctgacgca aaccctgaat ctgggttcgc atcttttcag
    74881 acactgccaa accagaagca tcatcgccgg ctctatttat tctttcgcct gatgaaagct
    74941 tctccatact cttgttcaca ctccattgtg tgaacttaag tgttctgtta gcgtttacag
    75001 cactcatgtt gtggttgata atcattttac actcctttgc acattagttt tggcttccct
    75061 gccattaatt acaccacacc gggcagacag tgcagtgtaa gggtgagtag atacaaagct
    75121 accctgttcc aggcttgtat ctgggctcat acaagaccag aaccattaaa aggccaagcc
    75181 tggagtgcta ctatcaaacc acaaacccca acaggtgttt tcaaagacca atgtcaggag
    75241 acactactcc ctgctcgact tttcgtcacc gtaggcctaa aacatgaatg ctaaaatttt
    75301 gtgtattttg ctttttatca gggaataatc gtatttattg caaaatatga ttttattttc
    75361 tctttttttc tctaaatttt aatattttta tattatatgg caagaggtgc ccttccacag
    75421 cattttttaa attttttgcc actaccacac gggcaaatgt cattacgctg taactggaaa
    75481 aactttcctg tttgtactga tttctgtaat ttactgaccc tttctgccaa ttccttttca
    75541 tagtgctggt aatgctcaaa aagcatcttc cacgcttcac acaatttact tgtgcctctt
    75601 tgatgtatat cagtagtgaa tctatacctg gtacaatcgc ccatacaaaa attattaatg
    75661 ctgcacatcc tgcattctgg agttgtttca cgttttagct gcgcaaatgt atttcgcttt
    75721 tcattattca aaatatcctg taagcgatag ttcataatat tacctaagtg ccactccgga
    75781 tacacaaaaa aatcacaggg gtaacagtcg ccattgtgct caacaacgat atatgaattg
    75841 cattcaccaa gccagcaaca tgacgttttt gcacccaagg aatacatgag aagatcctga
    75901 aacaaacgaa cagaaacata gggaaaacca tcctgatacc atgcatcaaa aagctcacag
    75961 taaaaatttc ccagatcctt tccacgtata ctgtatggca ttatatttcc cttttcatca
    76021 tgttcaaaac atggaataaa ctgaagatag gcaaaattat tgtggcggaa gaaggcatag
    76081 agttcacgtg catgtgtaac atttgcattg gttactaatg taaggatatt atactgcacg
    76141 gcatattcat tcataagagc tatcgcatgc ataacttttt caaaagtacc atggcctgca
    76201 taatctcttc gataggtatc atggattacc tctggcccat ccaggcttat accaaccaga
    76261 acattgttac gtgataaaaa ttcacaccat tcatcattga gaagaattcc atttgtttga
    76321 agtgaatttt ctatgatttg aacatctgtc tttaaacggt tttgaacagt aactatctcc
    76381 ctaaaaaaat caagccctgc taacgttggt tcaccaccct gccagcagaa tgaaatatac
    76441 gttgagccag taccaattgc tgcttttatt accgtctcag ctacatcaaa cggcattatc
    76501 tgaagactcc ccgggtacag atcaactacc cgtttgtaaa aacagtaatt gcatgctaaa
    76561 ttacaatgat atgacaccgg ttttactaag aatgaaaggc ttcccatatt agtaacagtt
    76621 atttccagcc acgtatattt gcatagaatt ccttttcaaa agccaaaagt ttttcatgaa
    76681 gttttttccc aatagctgga tagcgtttta taacattgta gctctcactt ttatcatatt
    76741 ccatgtcata aagaacaggc catgtagcca gtgttgggac tttttgattg gaaccaggtg
    76801 gttcgtaatc atgaccacct gcagctgacc caaaaaatgt atcaggttta tccagaggta
    76861 caggccatcc ccgtgtattg ttgtatcgta tatatttcca ccttcctgaa cgtattcctt
    76921 ctatttctct gtaatgaaaa aaatataatg catcatgagg tgattgcttt gatttgcctg
    76981 ttaacagagg cattaaatcc ttcccatcta taatacgatc tttgggtaat gtcaatcctg
    77041 ccagattgca tactgtaggg aaaaaatcaa tattcataac tggttgattg cacactgtcc
    77101 tttctttaat agtaccaggc caccatgcaa tcattggaac cctgaatcca ccatcatagc
    77161 tctccccttt ccttccacgc aatccgccag tactaccatc aaaccacggc ccgttatcgc
    77221 tggtaaatat aaccagcgtt tttgaatcaa tattattttt tcttagacat tgtagtatct
    77281 ggcccacact ccaatcaact tctgcaacgg tatcaccatg agggccagct tttgttgttc
    77341 cttcaaactt ttttgacggg aagcatggtt gatgcggatc tttatgtgct aaataaagaa
    77401 agaaaggttt tttatgattt tcatttataa attgtattgc ttcctgtgta aatagccctg
    77461 tgtatggttg ctgttcaagc ccaatatcct ctatgatcat ttttttatct ttaaaatatg
    77521 aaactggcca gtcatcatta cttgcattga aacccacaaa tttgtcaaaa ccataattgt
    77581 gaggatgata cgtttcatcc acaacaaaat cacctaaatg ccatttgcct atggcacagg
    77641 ttgcatagtt acgcaatttt aaagcctggg cgatagttat ttcagaatga ggcaaaccat
    77701 tgaccaaatt ttttgcacca tgtaaatcca ccacaccaag agcaccaaac atataaccca
    77761 actcttttac aattttgcgc ataaaggtat cttttcccgg ttgcaatggg aatgtaatgc
    77821 cacaccgcaa tgggtaacgg ccggttaaaa gccccgccct tgaaggtgaa caaagaggac
    77881 ttgcacaata aaaatcgata aatcgcatac cttcacgcgc cattctgtca atatgcggag
    77941 tgttaattgc tttattgcca taacaaccaa tatcaccata ccccatatca tcagtaagta
    78001 ttattatgat attaggcacg tttttttttt cttttatact gataacttca gcagctttaa
    78061 cagtgttctt ttcctcatat atatctgggt caatatctgt atcaacaggt atcaggcgcc
    78121 tgccacagct aatagtactt aatggcgata atgaaagtaa cgttgtagca gataatcgga
    78181 tcatctcacg gcgtgataca gtacgtggac ttagcataga acctcctctt tgattactaa
    78241 gatcattgaa tgttgcggtt ataattcctc actatccaaa cctgcctcag agaccatgta
    78301 acatgacgaa taataccttt tttgtccatg tggcacacct taaccaccat tatgaacagt
    78361 tcatgctatt atcgcaacta tttgactttt tttatcattc ctccaaatat acaaaagcat
    78421 ttaaagcata tactcacaca acgtaattgc aagcaaattt atgaggtttt ttctttattt
    78481 ttgttgcttt attcatgcac gaccattaca cttcatcaat tcttttttgc atgggagatt
    78541 tacaatgacc ttagatattc aattagtaga aaattcaaaa agtaccatgt tcccacattt
    78601 tatcaatatc ccacatatga tttataaaaa taatgatcaa tgggtaccgt ggttccataa
    78661 tgagatgaaa ctttttattg ataaaaagca ccctttgttt cttcactctg atggtgactt
    78721 ttttgtagca atgcgcaaca actccccggc aggaagaatt tttgtatttg aaaacaccat
    78781 gtataataaa acccataaca tgaagagtgc acatttttat tttattgatt tttatgatga
    78841 cagggaagtt gctgattgct tattcagtgc tgccatcgac tgggcaaaaa aaagacattg
    78901 taacagcctg cttggcccta tgggatttgg tggagtaacc ggtggcggtt tattaattga
    78961 tgggtttgaa caccgtgcat ccatgactat gatgatgtat aaccatccgt attaccgtgt
    79021 tcacatagaa gcatttggct ttacaaaata tttagacaat ttttcttttt accttccttc
    79081 tactgcgcaa ctgcctgatg gcttaaagca aaccgctgat gccttactgg caaaaggaga
    79141 attcaaagtg ttaaaattta aatctaaaaa agaattacag aaagttgcag atgatgtcat
    79201 ccatgtattc atgaaaactt taagtgatca tgctggaaac tatgaattga ctaaacctga
    79261 atgtgactat atagttaaaa gcttattaca ggttgccaac cctgaactta taaaaatcat
    79321 tacttacaaa ggtacaatca taggatttct ttttggtttt catgatttat cagcagcatt
    79381 acaaaaagca gatgggaagc ttactccttt atccatttac agaattatac gagaatacaa
    79441 aaaaaccaac tggatactgg ttaacggaat gggcatactt ccagaatttc agggacttgg
    79501 agccaatgcg gtattatatg cagaactgga aaaaaccata cggcaatacc ctcagtttaa
    79561 acatttagaa atggtacaga tacaggaaac tacaagtaaa atgcttagta atgttaaaac
    79621 attaaatgga aggacattca aaactcaccg gatgtatcag ttaccaatat aacctggcac
    79681 caaaatgaac atttcacaat atatgaatat caattactca tacaaaaccc attttgtaat
    79741 tcatcagtct gtattttatt tgacaatcca ttatcggttg aaaatattgc ttcaacaaca
    79801 tatatactat atcagtcaat ggagatcggc atgaaaaagt taaaagtttt ttgtacgata
    79861 gttcttgtta ttgtggcatt ggctatgcca ctggtgtgta caggtgctct ttacgctgaa
    79921 gcagcacacc ctgcagttac agcagaatcc gctgaaacat cacatggtgg acatgacatt
    79981 ggtaccgaat tgccattatg gtcagtactg ccatttgcag ggattttact atcaatagcg
    80041 ctttttcctt tatttgcccc tcatttctgg catcaccatt ttccaaaagt ttcagcattc
    80101 tgggcacttg tatttgcaat cccctttgtt atttactata aagggatagc tgcacatgaa
    80161 atcctgcata tttacatcat tgactacatc ccgtttatta ttttactatg ggcattattt
    80221 accatttcag gtggtattct tgtacgagga acattgaaag gtaccccttt aattaattcc
    80281 atcatattga ttattgggtc tttgcttgca tcctggattg ggacaaccgg tgcatcaatg
    80341 ctgttaatac gaccaatcct gcgctccaat gcatggcgca aacacaaagt acataccata
    80401 gtatttttca tatttctggt aagcaacatt ggtggttcat taacaccgct tggcgaccca
    80461 ccactatttt taggattttt acatggagtg ccattcttct ggacattaac ccatcttttt
    80521 actgtatggg gttttgcagt aataatacta ttattactgt attttatcat tgatacttat
    80581 ttctatagca aagaagacaa atccaaagaa gaatatggtg aaaagcaggc gcttaaagta
    80641 gaaggctggc acaattttat tttcttagct ggtgttgttg gtggtgtatt attcagcggc
    80701 ctggttaagt taggtgaagt caatatatta ggtgtacatc aatcaataga aaaccttatc
    80761 agggatgctg tactcatcat catgggatta ctatcactta aaactacttc tcagcaaata
    80821 cgaaaagaca atgaatttac atggtttccc attcaggaag ttgcatacct gtttgctggg
    80881 atatttatga ccattatccc ggcccttgca atattaaaag ctggtgaaca tggtgcctta
    80941 gcgtttttga ttaatgcagt aaaacagcct gctcattatt tctgggcaac gggttctctt
    81001 tcaagttttc ttgacaatgc cccaacatat ttaacgttct tcaatacagc actgggaagc
    81061 ttttatgctg gcatgcctga aatagaagct gtacaccgac tgatagcaga acaaaacgta
    81121 tatttacagg caatagcagc aggtgcagta tttatgggtg ccaataccta tattggcaat
    81181 gcccctaatt ttatggtaaa atcaatttca gaagaagcgg gaatacgaat gccaagtttc
    81241 tttggttata tgtttaaata ttcaattccc attcttattc cggtttttat acttgtaacc
    81301 tatgtatttt tcagataata tacttttgtt ggatttgggg aaatagtttt ctctgatcta
    81361 ttaacaataa taataaacga gaggtggtat tatggcaaaa acattttatg aagtggtact
    81421 ggaaggccat tataactttg tatacggtat gcttgaggga tataaattag ctgcaggtaa
    81481 aaatttcatc tattatttaa gccagcaggt taatgtaaaa gcaacaacac tttctgaggt
    81541 acttaaagaa ttcctgaccc ttaaaagcaa gttacaatat gttattattg aagctaattc
    81601 atggaaaaaa tttgaagccg ctgtcaaaaa acaacctgaa cattcaatca ttaataaaaa
    81661 gtatattaaa tcaattaaaa aaattgaaag tgcatcattt gaatttaact ttgaatgcta
    81721 tgctaagaag tacggtgatg aaataaaggg tatatttaaa tcattaccta aatcagttaa
    81781 gcttattgac tatgcaccaa aagaagaaat tcacaaagaa gcaaaaggaa ctgagctgta
    81841 tgcacctgac catgaatatt cattcaaagg aaagggcaaa ctggtgggtg acattgaaac
    81901 aatcattaca ttcaacaaaa aatttgacga acatccctta atagatgttt ctgctatcag
    81961 tttaaacctg aaataacaat gcgttcactt cccggctatc tcagactgct gggaagtgaa
    82021 cattactcta catgttgttc agggattttc attcaggaat caatagtata ctccgatatt
    82081 aaatacgtat aatcatatag taaactattt tgatggataa aaaaacttaa aaaccatgaa
    82141 aaaaacatta gcagttatta tcattagcat tatttgtatt gcttctacta tcatgtattc
    82201 aaagtacaga atgtcatcgt ttgaacccca atatatagga tttcttactg ttgaaaacag
    82261 caccattata aaacaattag atacagtatt acaaaaaaca aatatagcag gtatcagaag
    82321 tgataaagaa ttagcaaaat gtttagagga acagattaaa tccttacagc aattagcact
    82381 gattgcaata actgataatg aatacagtgt tcttatagca gctaaaaaag atagagttat
    82441 taatcaaaaa ctttttgatg caattattac cgatatttcc cagaaaaaac tattgccatc
    82501 aacaacattt ccacaaatca cacgatacta cagcaataat aaatattatt tttttattta
    82561 taatgctccg cagtatacaa tcatcacaac atatagtttt gtattgccca aaaatcttat
    82621 tattaaatta gctctcgaaa accttctcat tgtcattatg tttatcatca ttggtactgc
    82681 tataattatc acacttcaac agaaagctaa agaacctgta actatgaagg tggacaaaac
    82741 agaaataaaa ccacaaacgc cagaccccgt tggaagtcta tgccagcagt tttcaattat
    82801 ttcagtaact atcgcacaac aaaataaaaa tggaaacctt aaaactattt acagccaccc
    82861 tgataaaaag caaaatctat acctgcaacc agaaataatt aatgaattgc tcaatggcgt
    82921 acatatactt gcagaaaaaa atactgtatt aattttttat aacatcacca ctaaactttt
    82981 tttcagactg gctaaacatc aggcttttaa aggaaaaaca ataaatgata ttgaaaggtt
    83041 tatagacaat aataatgacg tgcttcttaa atttttgaaa ataaagaaat aacttgtttg
    83101 catttaccat tagcgttttg tagccacaat agcataatga tattgtcccg cttgcagaat
    83161 attcacaatt ttaaacccac gttgatacag tgttgcagta gcctggtcaa ccgataactt
    83221 gatatccatg gatggtccag gaataccctc tcttttataa aaatcaataa ttatcagtaa
    83281 ccctttatca ttcaaagact gtattgtatt cttcattgca gcatcaattt cattattgta
    83341 gacaagcccg tgcagaacat taatcataat gactgcgtca gctttaacag gaatatcttt
    83401 tgttatatca ccaggcattg gctgcatatt tgtaagccgt ttttctttta ctacgatatt
    83461 caggttatct attgcctgtt cattgtaatc catagcataa attgttgaat caggaaactt
    83521 ttcagaaaga gcaattgaaa aatatccatc accacaacca gcatccagaa tatgcattcc
    83581 atttgttata ggaagctttg caataagttc atgtgcattt atatatacac tgcttgattt
    83641 ccctttgtgt tcgtgttttt gcattggatc tccttatgat atttgctttt aaaatgtttt
    83701 ataaatatcc acaaaggcaa ttacataatt acccctgtgt aatatgccct attataattc
    83761 tattgtaacg gaaaattatg tatcaataaa caaatatttt acagtggaca taacaatatt
    83821 gcactataat acttggtaca tatacggtat gaatagtata ctttgaagat atcaccttta
    83881 ccatgaaaat ctataagaat atttccatta tttacgaaga taatcacatc attgttgtag
    83941 aaaaacctgc aggcatgtta agtcagaagg actatactga tactgattcg ttgcttgaaa
    84001 ttcttaaaga atatatcaaa accacctata aaaagcctgg caatgtattc cttggtcttg
    84061 ttcacaggct tgacaaacag gtttccggaa tcatggtgta tgcaaagaca tcgaaagccg
    84121 cttcacgtct ttcgtctcaa ttcagaagcc atgatgtcat caagatatac tgtgcggcac
    84181 tacaaaccat caccagcact gatactgcat ggcatacaat ccatgatgca atcatccgac
    84241 accatgacaa aacactgatt tctagtgata acattactgc catgcatgca tcactcaaat
    84301 ataaaattat ttataccaaa ccatcctatt cactggcact gattcaactg tatacgggca
    84361 aaaagcacca gatacgtgca cagatgtcgc atcttggcat gcccattgcc aatgacatta
    84421 aatacggagc aactttgata tcatcggaaa ctgcaattgc cctgcatgcg gtgtacctgc
    84481 aatgtattca tcctgttaca aaagaacgta tatcctttta ttccaaacct caaggcatta
    84541 ttgcttcaat ttttaatacc attgacgttg accatgtaat taaaaatgca ttgatccatg
    84601 aaggctattc actataacat ttagggcgat agtaaccatg ataatcagtg caagccgaag
    84661 aactgacatc cctgcattct attcacagtg gtttattgat tgcatacaaa aaggtcattg
    84721 ctctgtcacc aatccattta atcccaatca gagaaaggaa gtatcgttgc tgccatcaga
    84781 tgtcgatgca atagtcttct ggacgcgata tcctgtacca ctcatgaaat atttaggcca
    84841 gttgactgat tcaggatacc gttacatctt tttatacacc attacaggct atcccagctg
    84901 gcttgagcct cattcaccag aactatcgca tgcaatacag gcatttaaag atgtaagcag
    84961 gatactggga tcacaaaaag tagtatggcg ctatgatccg ctagtatttt ctaaagaatt
    85021 ggattttgat tttcacataa aaaattttgc tgcaatagca caacaattac agggctacac
    85081 acaaagagtt attatcagca ttatggaacc atacaaaaag gttacacgca ggctaaaagt
    85141 attgcatgaa aaaggaattg taacataccc aatgaatatt tttgggcaag atgaactttt
    85201 caagtttttt acctgcataa cgcacatagc gtttgattgt ggcatgcaga ttcatagttg
    85261 ctgcagtaat ctcactgagt ttggaataga aaatggcgca tgtattgacg gcaaccttat
    85321 caattctatg tttgggttag atttacattt tgaaaaagat atataccagc gtaccaattg
    85381 cctgtgtgca aaaagtgttg acattgggac atacaataca tgtaagtttg gttgtttata
    85441 ctgttacgcc agcaggtaat aaaaacagta actatcgccc atactacttt tcaaaccgaa
    85501 atgacctcac tatctttaaa aacgtttctt cataacggtc aaatgtatga ggtagtgctg
    85561 tacatgtaat aacatacact ttattgttat tgaggcaata atactgcaac acttttaatt
    85621 ttaacccact catagtatat gtaaatccta aaatcattgc aggtttgcgt gccagccacg
    85681 tgttgttttt atacagtagc ctgtaatctt ttgaatagct ttttaaattt ttttgtgaaa
    85741 gctcaaaata tttatcaaga gtactatcac ctgaaacttc ttcaactgtt atggcaacat
    85801 tttctctgaa atcatcacgc attccctctt caggactcaa tgcaacaaca acagcaccca
    85861 tttcctgctg ctgtatctcc cagtgttttg ggaatacaat ggaaaaatta tattcatcac
    85921 tgtaaaacct gcccttctcc ttccgtgaac aaccgggtag tatcattaca ctgaatataa
    85981 tgattgatat acatagtaag tattttcgtc tcatacgttt gctccagatt gaatagaaca
    86041 tatctgtata atactatata gtgtatactt catttattat aatggtatat tacacattct
    86101 ttaattatat cataaaatat tgttaaccat tattagcaat gctttttctt tcatttaata
    86161 ttctttttat ttcatcaaac ccttcccgtg taatgggtag tattgaaaca aaatacaccg
    86221 aaattaaaaa gcacaaacct ggcgcaaatg ccatcatata tcgtatagct gaaactgcag
    86281 attgtggctg tgctgtggca ctgttttcaa tatatccaaa aaaagccaga aaagctgatg
    86341 ccagtgcaat agctgatgct gaagcaacct tatataaaaa cgttgtaatg ccatagaaag
    86401 caccttcacg tcgcacaccg tttttatatt catcatattc aataacatca ggtatcatgg
    86461 aaaatgggat tatctgtgat gctgatatgc caacccctgc aagtacacca atgattatta
    86521 caaactgcaa attatgtact ggagctatca aacataacaa aagcgatagc gtaaaatata
    86581 tagcagcaac catatatgct ctgcgtttgt caagtttgtc tgaaatataa atccacaatg
    86641 gggctgatag taccgctaca atgagaggta tacccataac aacaaaagtc atgtcttctt
    86701 caatcagaat aacatctttg aggaaaaaga tgaatagcgc ctgaatcaaa tcaaacccaa
    86761 tcatattaaa taaaaacatt cctaaaataa tcctgaattc atgcaaactt agaacagctt
    86821 taaacgtttt caaaagacca tcatgtatga ccgcttcaga ctgaactcgt tctttagttc
    86881 ccacaaatgt tatgaaaatc aatactataa ttatgatacc aaagataaca cccataacag
    86941 gatagcttgt aatatagtgt tctttgcctt taaagactac atctacaatt accgaaacac
    87001 caactgctgc tatcagattc ccaacaaatg aaagtgacat tctaaagcct gatatgcttg
    87061 tccgctcatc gtagttttgc gatagctccg gcatcagagc attgtaggga actgccacta
    87121 cagtaaaagc agtattaaac agtatggtga ttgctgcgta ataacccaac agtatataat
    87181 gatcatgaga gggaaccagc catattaaca tgaacataag accaaaagga atgcaaccaa
    87241 acagaaaata aacccttcgc cttccatatt tagatttagt cctgtcagaa atcataccca
    87301 tgagcggatc cgttatggca tcccatgcac gagcaataaa aaagataatg ccagcccact
    87361 gcgcaggtaa gcctgcaaca ttgactaaaa aaaaataaaa agtaaaaacc tattgtggta
    87421 taggcaatat taattgctaa atcacctaac ccatatgcaa tcttttcaat agtggggact
    87481 acataacctt ctttcattgg caaatacctt tttatattat tgatatatat ctgaataata
    87541 aagaattaca tgctatatca aacatagaaa tttacataaa ttattagatt ctgagtccca
    87601 atgaaatcgt gatgaagaat ctcgccgttt cacacaccgt atttgcggtt cccgatgaag
    87661 ttagaacatg gtaatcccat ttatatacct gagactatca cacattgctc gctgtaacgt
    87721 gcaatcaacg ttattgcaag tcacaacgta ataccctcac tgtcatcctg aggagtccaa
    87781 actttaagtc ctgatgcaat cccgatgtaa tcgggaagga tcttgccgtt ttattacttt
    87841 ataactgaga ttcttccccc aattatatcg gggtcagaat gaaaattctc gttgtctcga
    87901 tatatgcctt gcttagctcc aaacactcga cgaccttatt atgaaaattg atcaccaagt
    87961 gcatgcaaag cacgtatatc gaagtgaccg atatgtgaat attgttaata caaaactata
    88021 gtggcaagca aaaatcaagg aaaataaatt atttatttcc aggcaaccag actaatactt
    88081 tttactatct ctttatactg ttcttgacca tcatatgatg tgccatcatt gttacattca
    88141 ttacttgcaa aaatattgcc ataaccatat tcttttacaa tttcaacatt tgaaaaaccc
    88201 gctttatgta acaacgcaat atactcactt tttagaattg ccccagctat acaacttaca
    88261 tacgcatcaa tagattcttt aatatatggc ggtaaggttc catcaagcac aatgtcggat
    88321 atagccattc taccaccacg tttcaatacc cggtaagctt ctttgaatac ccgttcttta
    88381 tcaggtgcta aatttataac acaatttgaa attatcacat caactgaatt gtcatcaaca
    88441 ggtaaatgtt caatatcgcc cagtatgaat tcaacattat taactcttag tttttttgca
    88501 ttttcttttg caagtgctat catttcaggc gtcatatcga caccaataac cttgccatgt
    88561 tgccctacct tttttgctgc aagaaaacag tctatgcctg caccgcttcc taaatcaaga
    88621 actatctccc cctgtttcaa agaagctatt gcattgggat tgccacatcc cagccctaaa
    88681 tttgctgaat caagttcatt catttcatta gctgaatagc caattttgga agcaacatct
    88741 ctggggttgc aacaaccagc agaacaacat gaagtttttg aaagggctat tgtgccataa
    88801 tttttttgca cgattttttt aatttttgta ttagtatcca tactcatttc cctaaattgc
    88861 tgaatactgg agctatctgt tcaaaaacaa ctgaatttaa tgaatcggcc tttacaaaag
    88921 caatacaaca taacgactca gtactattaa gagttaaaat gacaaacttg tctcctgctt
    88981 ttatcccata cttttccctg agatcttttg gcaatactaa ttgcccacgt tcatctaccg
    89041 aaaccactgc ttcgattgaa cacgaagaat tgcaacagtt attattttgc atagttttat
    89101 ccttattaat catgatataa tgttttatca gaatattctg tattttctga tatttctgat
    89161 attgattatt ttattgctgt tttgtcaaca aaattttcac aaattctttt ttttgcagaa
    89221 atgttatatg tgctctgacc ccttttactt ttagcagaaa gataatttga caatctcatg
    89281 ctctggcact gttcacttta tttcaaaatt atcaatatca taaacctaat atattttaaa
    89341 caaaagtagt ttcctaatac tttgtattaa aagaaaaaat aatcatttgt ttttttacta
    89401 cattattatt aatttgttta gcaagaaaaa tatacactta tgaaattatg gaggttccca
    89461 atgattaaca aaattatggt atttattata gttgtttata ttacgagtag cagtgcgtat
    89521 gctttccttg aaaattataa caactatcaa tatctcattg gtgatagagc agctggtttt
    89581 ggtggagcat attgtgcaat tgccaatgat agcactgcgc tgtggtataa tcctgcaggt
    89641 cttgcaaata ttaatgacaa tagattgaac atatcagcca atacctatag ttatcttacc
    89701 cgaaattcca gacaatactg gcaaatagaa gaatcacctg gacaatatca gtgccttgat
    89761 ttaaaagaaa ccgatatttc agttgtgcca aacaccgttg cctatgcccg cagagtaaac
    89821 tggctgggaa atgatgttat tgcttatgga ttatttgttc ccatccagga ttcaatacag
    89881 gcaactattg ctggcaaagc aacaggtagt gtattaaatg ttgaacttaa tgcttcatat
    89941 agaatcaata caaaatcata ttatggggtc gcaggatatg gagttaatat cactaaaaac
    90001 ctgaaacttg gtttatcggc aggtgttggt tactttcagg ggaaaggtgg tgcaaacatt
    90061 tcagcctatc ttgatccagg tggaacaaat caaagtgaaa ttgcaatgat tattgacaat
    90121 gagcttacag cagttactgc atttaccggt actggcctgc aatatgctct gaccccccat
    90181 cacagcatag gtgttttcta ccattctccc atatatagat tgcatgcatc aagggaagaa
    90241 agaactacaa gttatcaagt tgggccaatt ttcccaacaa atagctctga aaccacaata
    90301 aataatgatg actttcgttt tagaataatt ccagcatata tttcatttgg atacggatac
    90361 atcagtgaag gcaagtggtc atgtacggtt gaaggtgttg ttcacaatac aaatacagat
    90421 tatcccaata gagttataaa tgggaaagtt gggttggaga tatatttact tgaagatctt
    90481 attgtacgaa cgggcttttt taccgatttt agccagaaag atacagtaac ccaatcttca
    90541 gaaaatgacg aaaaaaatga ttactatggt gcaacgttga gcctttcgtt tggcaatgat
    90601 cttaatccgt tgcaaagtga caccctgaaa gccaaaaata tgtggacaac agttggaata
    90661 gtataccagt tagggattgg tgatattcgt ggtttcagat tttataccgt tacttcgggc
    90721 tctgatcctg taatacacca atatacccac cgtataagcc tgtatattgg tgaatcaatt
    90781 gtcttttaaa aggtattgac tttaataact atattgttta tagtaaactt cagtttatta
    90841 ttacgaatac caccatatag gcataacatt tttaaggaat tttcattata ggaatccttt
    90901 tttagtaata agtaactatc tgatacaaat agtataacaa ctatcacatt atcatttgag
    90961 gtatgttatg ggaattctta aagatgcagg tgaatcactg gtaaatttta gcgaacggtt
    91021 tttagataaa actgaagagc tggctcaaat tgcacgcatt accatggaaa taaaaaagtt
    91081 ggaacattcc ataaaagaga tatacttgaa tactggaaag tatgtgtatg accaggttgt
    91141 aagtgacagg cctatttcaa atatggatga ttttatcata aaagctgtcg ccacaataaa
    91201 tgactataaa acaaaaatac aggaaaaaca gaacgaaata caaaaagtta aagaacacta
    91261 tgaatccaaa taccatcgat agtaaaagct aaaatatact atgtcaaaaa catcatcgcg
    91321 cattgtacag gctattgaaa aacaaaaagg tttttttact ccaaacgata ttgtgtcagc
    91381 agtattgctt tccacatcaa aaagtaaaaa atcaaagaaa agcactaaag tatcacaacg
    91441 tcaggctcag caaacagcta accgtgtgca gaatgctatt tccacactat accagtgtgg
    91501 atttctgacc aaacaaaagg gcaaatacag ggtagcacag ccattcagct tcacgggaac
    91561 atttattcat agcaaaaaag gtgacgggat tgtgtatttg catgatgaca ttgaagtaca
    91621 catttaccgt gaagatgtca atcatgcccg caacaaagat atcgttcagg tacagttaac
    91681 tgatatacgt cgtggaacgc tctttggcag ggttacgtca attgtgacag ccaacaaaca
    91741 gatgtttttt gcacgatgtg taaaaccaaa cggcaaattt cgtattctgc agcttattga
    91801 tgttccaggt aatattcagg tcattacaaa agaaaatata aaaccagggg ttctggcgat
    91861 agttactgta aaaaactctt ttatccaaaa ttatcaggaa tgcaccatca atgcattaat
    91921 agaacacgaa gaagaatatg acgtacagcg cattgtgggg aagttttctt tgcctggtgc
    91981 acatccagaa tatgaacacc ttgaaaatat tgaacagaat gtacatccac acgaactgaa
    92041 aaatcgcaaa gattaccgaa agcttttaac agtaaccatt gatggagaaa cagcaaaaga
    92101 ttttgatgat gccatttcaa tagaatataa aaatggtgtt tatacgttat acgttcacat
    92161 agccgatgtg tctgcttatg tatataaaga tgatgctatt gataaagaag catacaaccg
    92221 tggaacaagc tattacttag gcaataccgt aataccaatg ctacctgaga agttatccaa
    92281 cgacctgtgc tcactcaaag ccggtgttga caggttaacg ctttcggtta tgctgcagtt
    92341 tgataaacac ggcaactacc gaacacatga aatttaccgt ggaatcataa acgttgacca
    92401 gcgcttaacg tacaacgtgg ctcaggaaat attgaactca aaaaaacgga gtaatataaa
    92461 agcaatgctg ggacatgcaa aagacctggc acaaaaactt aaagcaaagc gattatccaa
    92521 cggaagagtt gacctgaact tagctgatgc cgagatgata tttgacaaca acgcttctat
    92581 tgctgatatt gcttttgcca cacgacttaa cagccacatg attattgaag agtttatgct
    92641 aagcgccaac gaagttgtat cacgtgtttt gcgccaaaac aatgtgccaa ccctgtaccg
    92701 cattcatgaa ccaattgccc aggaaaaatt tattgcactg cagaatttcc ttaagacact
    92761 gggcataatt cttaaggaag aagaaaatgt agggcttgct ctgcaaagag ttattgattc
    92821 agttgctcaa aaagagtatc agcaggtagt taattttatt gttctcaaat cactgatgca
    92881 ggcatattac ggacctactc cgttagggca ctttggattg ggctttaagg attatacaca
    92941 ctttacttcc cctatacgac gctatcctga cctgattgtg cacagatgca taaaatcatt
    93001 aatagataga gtacaaccgc cctattcccc tgatgaatta gcagtaattg gagagcaatc
    93061 atcaaaatta gaacgtttgg cccaatctgc tgaacgcgac cttttgaaac tcaaagcatg
    93121 cagattgctt gaagaacggg ttggtgagga atttgaaggt atcataagcg gtgttacacg
    93181 atttgggttt tatgttagtt tgcttgacaa acctatagaa ggtatggttc ctttgaaatt
    93241 cctgaccgat gattattatc tggttaatga agatgattat acagtaatag gcaggcgact
    93301 gggaaggcgc ttcaggcttg gtgaccttgt taaagtacgt cttataaatg ttgatactga
    93361 ttttatgcgt atagattttg aagtagtgta aaaggtacag tcatgctaac attacaggca
    93421 aaaagatttg aaacaatatc ttcatcggat actgttgcaa tactatgcac tcaggaacag
    93481 attgacacca attcgttcat cccgcagcaa ttatctgatg tatttgcaat tatcaatact
    93541 aaaacattta aagctaaacc caatacctat ttttttgcag cactcaaaga cagaccgtca
    93601 atccttcttg ttgggcttgg cagccacaaa gacatcacac ctgaatcgtt gcgcaatgct
    93661 gcaagtgtta tcgttggtgc atgttcagat aaatcagtat cggaagtaca tattgttgtt
    93721 ccggaaatta atgttaccaa cgacacagca cggtatattg ctgaaggggt atatcttgca
    93781 aactatgcat tcaatcgcta caaaacacaa aaagaagaaa ccagcccatt attggaaaaa
    93841 gctatccttt tgagtgacaa tcctggacaa ctatcgccac tattaaaaga agtaagcatt
    93901 gtatgcagta acacattgct atgtcgtgat ttggtaaatg aaataaccga gaatgccaat
    93961 ccgttaacta ttgcaaaact tgcaaaaaaa attgctgcac aatcaaaaat aaaatgcact
    94021 gtatataaca aaaaagatat tgaaaaaatg aagatgggcc tgcttttggc ggtaagccag
    94081 ggtagtaagt atccaccaca acttattgtg atgcattaca ggggtgcacc ttccaaaaag
    94141 gactcaattg ctcttgttgg caaaggcata acctttgaca caggcggcat taatttaaaa
    94201 ccatcaggac acattgaaac tatgcgcact gatatggctg gtgcagcagc ctgtttatat
    94261 accataaacg ccattgcaca gatgaagttg ccaatcaatg tatatgcggt tataccactt
    94321 gctgaaaata tgatttcaca caccgcatat aaacccgggg atgtatttac tgcatataat
    94381 ggcaaaactg ttgaaatagg caacactgat gctgaagggc gtctgatact ggctgatgcg
    94441 ttatctttca ctgaaaaaaa attaaaacca aaagctatca ttgacattgc cacattaacc
    94501 ggcgcatgta tcgtaagttt tggggagata gtagctgcat atttaaccag tgatgaatca
    94561 ttatccactg cgctgtttaa tgcttcagaa tctaccggtg aaaaaatatg gcgtatgcca
    94621 ttatataaag aatacaatga agaaatcaaa tcagatattg ctgatatagt taatgtccct
    94681 gctgtacgaa atgctggaac aattattggc gcaacatttt taaaaaattt tattgaaaac
    94741 actccatggg ctcatattga tatagctggc acatcatggt attctaagaa gcgtggatac
    94801 aatccaaaga atgcaaccgg atttggtgta cgattgttat atgaatttgc aaaaagtgtt
    94861 gccttgaaat aaggaaatcc gtatattaga ttacactatc acaatggagg agcttacatg
    94921 tattcaacaa aagaattaat agacatagcc attggtattg aagaaacagg attttatttt
    94981 tattccacgt tcaaaaataa atttaaagat gaatcattta aacagatttt tcagttctta
    95041 gccgatgaag aattacggca taaaggcgta ttttccgata tgcttaagga attaaaagac
    95101 actcagggtt tgtttaccga agaatactat tcctatttga aggcaatagg cacaacacga
    95161 gtatttgcaa ataaagatga tattgataaa atagcacctt cacttcatca gccattagat
    95221 ataatcaaaa tagcaatgga cgctgagcgc gattctatag tatggtattc tgaattgaaa
    95281 gaaatatacc gcaatgatgc aaaagcaaaa aacatactgg aacggcttat caatgaagaa
    95341 cgcaaacatg tacttacgct tctggatttg aaagagaaac taatcatgta gcatgatcta
    95401 atcattttct agttatgcta ataaatcatt atggtgcact caattaattt ccgatcacga
    95461 acgccgtaac ctggtataac agtgccacca tgagtgtata ttgtcttcac cgtttgcccc
    95521 atgcaatcgt attgatatgt ggttggatgt gtcattgaca ctggtgtata ggcagtgctt
    95581 cccctgcctc aaaatatggt ttcccctgcg caatgactct cccctgagta tcgtacacat
    95641 ttgctccgct taccgtaaag ccataactac cgttcacctg cgcctgcgtc tttacctgca
    95701 tttccctgcc aggtccatca gcaaatcgca ctaccgctat cacctcgttg taacctgctc
    95761 cactgcgatt gtgtgtggcg atagttactg gtgtactaat cattgcctgg tattatcgat
    95821 taacgaaata ttttgtcttt agcaattaca tgtgtctgaa acaacaaact ttatttttga
    95881 gtatattcgc aaatactgta ttgctacatt ttcctttctg cgcttaaatt gtccttgaaa
    95941 attgagtcag catatcatag taaagctggt acacaaatac ttctggaggg gaatcatgaa
    96001 tatagaaact accacctgca ttgcccatga aaatttaaaa ctgctacagg attatgctga
    96061 tatctacaaa ctatcgcttc acacgtttat tattaatttt attaactatg tgatgtcata
    96121 caaaaagatt ccggtgaaaa gctgcaagcg gcttacatac cgaaagcgct atgaaagctg
    96181 gaagcgggtg catctttatc tatatgagaa tgagtatgag tttttgatgg atgcgcgcaa
    96241 agtgtgcaag atgtccattg ccaaggttat ttcctactgt attgaaaact atctttttga
    96301 ttttcttgca gcacttgata gtgaggataa taccgataac tatcgattta gtggctacac
    96361 atttatgttt tatttagaaa atggtataca atgctgtcga ttttactggg gaccacaccc
    96421 tgaattggtg aaatacgtca tgcagtaacc acttacattg ttataagatc atgcaacgta
    96481 aaatttattt tttcctacca aagttaacaa ggggctacct ttctattacg taaattctaa
    96541 attttgtaat actacagata tttatcattg acaaataact ttagccaaat aatttggttg
    96601 gcaaccaata ttttacatct ccagccaaag tattggtctg gtcaaactaa acagatacta
    96661 tttaatccat agaaaggggg attggaagcc tatgagtttg tatcaaaaag tgtatgatga
    96721 atctattaat aatccggaaa agttttggtc agaggccgca aggaatattc actggtataa
    96781 accatttact aaagtacttg atgattcccg taagcctttt tatcgatggt ttgtaggtgg
    96841 acaggtcaac acatgttata atgcgcttga ccgccatgtt gaaggtggta agggtaatca
    96901 ggttgcactt atatacgata gtcctgttac ggatactatc gcaaagttta catataagga
    96961 gatgcttgat aaggtttcaa tatttgctgc tgctttacag aaatatggga ttaaaaaagg
    97021 tgaccgggtt attatctaca tgcccatgat acctgaagca cctgttgcaa tgcttgcatg
    97081 tgcgcgaata ggagcaattc actcggtggt gtttggtgga tttgcaccaa atgaattagc
    97141 aatacgaatt gatgacgcta aaccaaagct tattgtatct gcttcatgtg gtattgaagg
    97201 taaaaaagtt atagaataca aacctcttct tgacaaagca attgaaatag ccaatcataa
    97261 acctgaacat tgcattattt tccagcgccc tcaggtcaca tcacctctta tgccgggaag
    97321 agatatagat tggaatgatg ctataagcgg agtgaaacct gccgaatgtg taccggttga
    97381 atcaaccgat ccattatata tactatacac ttccgggacc actggaattc ctaaaggtgt
    97441 tgtgcgggat aatggcgggc atgctgttgc attatactgg agcatgaaaa atatctacgg
    97501 tattgaaccg ggtgatgttt ggtgggcagc ttctgatgtt ggctgggttg ttgggcattc
    97561 atacattgta tatgggccat tattgtatgg tgcaacaaca attctgtacg aaggtaaacc
    97621 tgttggaaca cctgatgcag gagcattctg gcgtgttatt tcccaacata aagtaaaagc
    97681 attattcact gctcccactg ctttcagagc aattaaaaaa gaagactcca gtggagaatt
    97741 tttcaaaaag tatgacttat ctcagttcaa atatttattt ttagcaggtg agcgccttga
    97801 cccggataca tattactggg catcaaatct attacaaaaa ccagtcattg atcactggtg
    97861 gcaaactgaa accggttggc ctattgctgc aaattgtgtt ggcatagaat tgctaccaat
    97921 caaagcaggc tctccaacta aaccagttcc aggctatcag gtacatatta tcaacgatga
    97981 aggcaaacag ttgcccaatg gaactgaagg tattgtcgta ataaaacttc cacttcctcc
    98041 cgggacactt ccaacattat ggcagaatga cgataaattc cgtgagtcat atctggacat
    98101 atatgatgga tattacttca ccggcgatgg cggatactat gatgaagacg gatatctgtt
    98161 tataatgggg aggattgatg acgttatcaa tgttgctggc cacagacttt caacaggtgc
    98221 catggaagaa gttatttcaa aacatccaga tgttgctgag tgtgctgtca ttggagtgga
    98281 agatagcttt aaaggccaat tacctgttgg atttgttgta ttgaaagaag gcacaactaa
    98341 aagtcatgat cagattaaga atgatctggc acagatggtt cgtgagcaga ttggtgctgt
    98401 tgcctgttat aaaacaacac tgatagttca gcgtttaccc aaaacccgtt ccggtaaaat
    98461 tctgcgtggc accatgcgaa aaattgctga taaaaagcct tatacaaccc catcaactat
    98521 tgatgatcca gcaattctgg atgaaattaa ggacgctcta tcaaaggtaa tagttagcgc
    98581 atagtagata aaataaaggg gtcaacttaa aaaatggtcg tagagagtat tctataagca
    98641 ttctattatt atccgctcat tttggtataa tgcggtcgtc tggatatgat tgctagtgca
    98701 atcaccagac ggccgctcta tataggtcac agactgccag atatttgcca caaaatgtgg
    98761 caaaaatatt tagacgagca caaagaaatt tttatcataa ctactttttt taaactacct
    98821 cttttctatt caattttatt tgtatttgtc caaatattca tttccccagc tttcttaatt
    98881 aattcatctc tgaagtctgg atgagcaatg ctgatcaatg cttctgctct ttcccatgtt
    98941 gattttccct tgagctgtac tgcgccatat tctgttacaa catactgaac aatggatcga
    99001 ggaaccgtga caatggaacc ctgcggcaat gtgggtacaa tccttgatac caaactacca
    99061 tctttatcct tatatgttga agatagacaa ataaatcctt tcccaccatg agaaccaaat
    99121 gcaccaaaaa taaaatcaag ctgcccgcca gtaccagatt tatgagctat ccccgctgac
    99181 tctgaacaaa cctggctgaa taaatcaaca gcaatagcat tgttaaccgc aaccacttta
    99241 tcattgagag ctataattct tgggtcatta atataattaa caggatacga agcacatgtt
    99301 gggttgtgat gcaggaagtc atataattta tttgtaccca tggcaaaagt gtagctcatc
    99361 ttatacttat caatattctt ttttgcccct gtcactctac ctgcatggta taagtcaaca
    99421 tatgagtcaa caagcatctc agtatgaact cctannnnnn nnnnnnnnnn nnnnnnnnnn
    99481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    99541 nnnnnnnnnn nnnncctgca tggtataagt caacatatga gtcaacaagc atctcagtat
    99601 gaactcctaa atccttgagg tcactttcag caatcatttt cccaactaca ttgggcaacc
    99661 caccaatacc cagttgcagg catgctccat cttcaatctg ttccagaaga tgttttgcaa
    99721 tttgcttatc aatgtcagtt ggctccagtg caggtatttg tggtagcggc aggtttttgc
    99781 cttcaactat atggtccacc cgtgatatat gaattgattc ctggtttcct cccaaacagt
    99841 atgggacatg ttcattaact tctacgacta tcttttttgc ttttgttaca actgcagacg
    99901 ttactgaatt tgcaaggcca taattgaaat aacctcgtgg gtccattggg ccaacagtta
    99961 aaaatgctat atcaaaatct aagtattttc tgattacccg tgggccctga tgataggtaa
   100021 ttggaatata attacaaaga tgctgatcat ataatcttcg tgagatagtt ccaaaatgga
   100081 agtcattcaa aatgaaatgt ttctgctcgg gatcagcttc aactattttg ggtatgcgca
   100141 tataacaaac gcttctaaca tctacattgc gtaattcctc aacgcgtttt gataatgcct
   100201 catcaatagc ctcaggaaat aaaacaaatt caccataaaa cactctatcg cctgatttta
   100261 caaaattagc tgcttcttct gctgtgattt tctttttttc atattcccat ttttcgatat
   100321 cacccattga tcttctcctt tcttcaatag cgattgtact gctttatatt ttttgattat
   100381 cataacttta ccaaaaattt ataataaata tattgtcatt attacatcaa catattgttt
   100441 gatttattga tagtatcaat acaacatgtt ttatttatat tgaatatagc atatacaaat
   100501 ttttatttct aatttttata tacctgtaaa tactattaat caatagatat tgaataccat
   100561 ctggcaggtt tgtagcgcat ttagagcacc catgcatggc gcctgtaaat ggtcaattat
   100621 ttaccttttt aaatcgggag cgtttaaaga attctcttag cctccattca tccaatgaaa
   100681 gaacataggg gctgtcccaa aagaattcgt tgagtaaatt ttgcaaacat tatatcataa
   100741 tactcgctca tttcgataca attttgacac aaaatctggt aaaattactc aatgagcgtt
   100801 gcgaaatttg tatcgaaacg acttttggga cagcctccac cagtatttat ttctttaaat
   100861 cttgaagtgt cttaaaaatt cttatagctc tccattcgtc taatgtaaaa acaaactggg
   100921 tatccattta ggatacccac ctccagtatt tatttcttta aatcctgcaa agtcttaaaa
   100981 attctcttag ctctccattc gtctaatgta aagacatact gactttttga actggtacat
   101041 atatacctgt tgttttcata ttctttatgg ataaccagcg taatgtcttc tggtttttgt
   101101 gattcttcac cttttaccga aacggtaatt gaagcaattt tccccgtcaa tgttttggca
   101161 ttaacgtcag ggaatgaata tgctttcagc ggatcaaatg tatataacat ctccttaact
   101221 ttattgttat ccagtattac tgctggttgt tctttgcata tccattgctc ttctttttct
   101281 atttctttga gcttttcctg tttttctttt tgcgcattac cttcctcgtt cttcttttct
   101341 actacttttt tttgaagtgt taacgatgta tttttatacc ataactttac tgtatcaatc
   101401 tttgctatat caagctgaac aatatcttta ttccgcaatt catctactgt tttattgaag
   101461 tatgaatcta acgtatcttc tgcaaggtaa acctttggat cgccttcaaa tcgaacatat
   101521 gtgtggcgat aggtgctccc ctttttgcca atataaacat cacgtacaat ctgaccattg
   101581 ctttttgcct gaacccgtat tgcatggtcg ttgtcaagct catagcgttc ataatgtggt
   101641 ttgtcactta tcagatcggt aatcgcgagg tcctttatct tatttaccat attggcaata
   101701 atattcttat cgccttcaaa ttcatttacc ttccagctat cgccatcctg ttttatgagt
   101761 attatcgctt cccctttttt aagtacaagc tctgttactg ggctttttat ggtaggcagc
   101821 gtaactacgt tttttggtat caatgaatta atgataagat acccagccag aacagctatc
   101881 actgcaatac tgataagaac attttttttc atgtgcatac aaacacctct tatctgaatt
   101941 atctcatact ggaaaattgt tcttcaatgc gcctgcggcg aatagtacga cgccacatat
   102001 acagcacacc agcaagaatt accaatagag gtaatccagc aatgtttata cccttaatga
   102061 cactttttgc cgtatcagat gtttctttaa ttgggttgaa cgataggccc ttgcttcgca
   102121 tctgtggaat ggcatagttg ccctgcatat aatcaatcac gttgtgcatg aacactgcat
   102181 tgggcgattt tccttcttca tcaataatgt tacgccctgt tatttctgat gttcctgcca
   102241 caaatagttt taccggtttt acactttgtt tcaaagcttc ttcttgaaca tagtctttac
   102301 cttttgtttc ttttgttttt acatcaattt tgggctgctt atcggcaaag tagcttttta
   102361 aatttccctg caccaggact gctaaattat actgcttgag gcttgaatcc tgcggcgggt
   102421 ttgcacccca tggcaaaaag ttaatattac cctgctgtaa ccagctgtat ggcgatgatt
   102481 ttacaacata ttgtacagaa gctttatttt ccttaagcag tgtttcatca actgaaatgg
   102541 aagaagcttt taaaaatact atcttcttta aatatttagt gatttcatta tctttagaca
   102601 aaccttcctc agaaatcatt ggagcaaagt atattgttaa ctcacccaac ccacgctgct
   102661 gtgcaatgaa gcactgctta tccatcacta tatcacggcc aatcttaacg ccataatggg
   102721 caagcatccc ttcaagaccg gtaaataccg gtaacgcctg aggtggtcgc tggaacattt
   102781 gcataccctg catctgctgt tccacatacg aatcaagtag cattacagca ctacctccgc
   102841 gcatcaggaa ctggtcaatt ttatacaatt catattcact gaacatggat cgcggcccat
   102901 taataatgat agttgcaata tcatcaggta tttcatcttt tgtaatatca atagttacta
   102961 actcatacat gtcgccaatc atctttctaa attgtgcggc accattttgt tcatcattaa
   103021 tgtcgcgctc accgtgacca acaatatacc ccacttttgg attgatgctg ataagattat
   103081 caatagcggc atttagcgta tcctctaagc gtgtcaaatc cccaactgaa tactgcccaa
   103141 atattgaacg ggaaagtatc tgcactgttt caaacttatt gttgcattcc accacaatgc
   103201 caaccatgcc ttcaccaggt tcaactatct tcccttccat agtagtaaac cgcggccacc
   103261 gcagcgttgg caaaccatac atctgagcca atgtactccc ttgtggatct tgtaatggat
   103321 caatatagcg atactgtatt ttattgtaat ttcgtatatt acatttctga acctcagcat
   103381 acactcgttc atttaaattc tgcatccctg gaattggcag gttactgctg gcatacaatg
   103441 taactgtaat aggatttttc aatttaagca gggaatcgat cttgccattc atctttttaa
   103501 ttagcgttgt tatacggtac tccagtcctt ctggttcagt aatagaatca attttttcta
   103561 ttaaatcacc atggataatg gcaaggccca taaatgcgtt tctaaatttt acttgatcat
   103621 tacgtatttc ccgtatctgc acgggataaa taccaaaatc tgaggcagca tccttctgct
   103681 tttcaacatc aacaaattca taatgaaaat acctgttgcc agcattatca tattctacca
   103741 tcaggtcttg aagatagcgg tacacagcat tataaggtgc gggaagatcc ttattaaaaa
   103801 atatctttat ggttaatggt tcttccagtg atgatactac ctctttactt atgggtgata
   103861 gtgaatagac actattcttt gttaagtcta tacgaaaata taatgtaagc ccaactacat
   103921 ttattaacac aattatcacg ccatagagta tatactgcga atagctcttt tgtgtttcag
   103981 tatcgcctga tttggtttgc tcttttatgt tttgtattac ttttgtatat ataattgtaa
   104041 tgttactttt caggttttgc agcatatcaa taatgtttct tttgttcata actatctcct
   104101 cacctccagc tgatatatgc ttgccattga actaattgca attaccgaag caaaatatat
   104161 gatatttcgc gaatccacca caccacgggc aatgttctgg aaatggtagt cagcacttat
   104221 gtactcaaac agtgaaacca atttgggtga aataaagtat aaaaattttg ttataagcga
   104281 tagtactatg caaatggcaa aacctattat aaaagcaaca atctggtttt tagtcattga
   104341 tgaagcaaac acaccaattg atgcatacgc agcagccaga agcactgcac ccacatagcc
   104401 gccaataaca gggccaatgt caggtgaacc aacaaatgca accgatacag catagaccag
   104461 tgttggcgat atcatgattg atgcaaacag tgttgctgct aatatctttc ctgtaactac
   104521 ctggattgtt gaaagcggta gcgtcatgag catctcaaac gaaccggtat tgcgctcttc
   104581 agcaaacagg cgcatggtga ttgccggtat cacaaaagca aataaaagtg gtagcagttc
   104641 aaaataagcc cttagttcag cctgtccata taaaaagaat gttgaaaaga aaaggaaccc
   104701 cgctataacc agaaacacag aaattacaat atatcctatt ggtgttgaaa aataccctga
   104761 cagttccttt ttggcaatga gtgttgaccc tttcattatc tctttaaatt gctccataca
   104821 tccctcctta cataccgacg gttagttcgc ggaatacatg ctccaggctt ctgtattccc
   104881 ggttcatttc atataatgtc cagccactgt cttttacaca attgaataca gcaggacgca
   104941 aatcaacatc agctccggtt aaaacttgaa tgtagcataa ctggccttca tcacgtacat
   105001 gttgtacgtt ttttactcct gatatatttt ttaaagcatt ctttattcct tcaaatgtag
   105061 tgccaccaat aatacagttt attcgaatat cttttcctct ggcacttttt agctcagtgg
   105121 ttgaatcatc agctactatc gaacccttat caattattat cacacgattg caggttgctt
   105181 ccacttcctg caatatatgg gttgacagta ttactgtttt ttcctttccc agctctttaa
   105241 taagatcacg aatttcaact atctggtttg gatcaaggcc gttagttggt tcatccagta
   105301 taagaatatc aggatcatgt aatatagcct gcgctaatcc aactcgctgt ttatatcctt
   105361 tagaaagctc attaatattc ttgtgcatta cttcggttat gccacattga attgcaacat
   105421 cttttatttt ttctttatca tatatatgcc tgatatcagc cataaattga agaaagtcat
   105481 ataccatcat atccggatac agaggtgcag attcgggcaa atatcctata cgttttttaa
   105541 tttcaattgg attttcatta acaagaaact catcgacact taccattcca ctggttggct
   105601 gaagataacc agttagtata cgcagtgttg tggttttacc tgcaccattt ggccccagta
   105661 aaccggttat ctcacccttc ctgattgtga atgaaatacc atcaacagct tttacttcgc
   105721 cataatgctt taccagattc cgtacgctta tcatgtaccg ctatcctcct tgataaaata
   105781 ggattgtgat tttgttgtta tttttcattc caaaatatag cgatgaaaat attttttcaa
   105841 tatactacaa tgtatatatt gatgtgatac tagaaatatg aaaaacaaat gatgaaaatg
   105901 ttacacatta tttctgtgct ctgccataat catcttcaat gcgctctata tcatcttcac
   105961 caaagtattc acccagctga acttcaatat acatcaaatt gtgtgttcct gtattttcca
   106021 ctctgtgcag catattgtaa tctatatcca cacttactcc tgaagaaacc tcaatttcat
   106081 gcttatcaag agtaactaca ccctgaccgg acacaaagaa ccagtgttct tttcgctttt
   106141 tgtggcgctg gagacttaat cgtttttttg gatataccgt tatacgttta accttatggg
   106201 tttcagtatc cgatagtacc tcataaaaac cccaggggcg gtgttcggaa taaattgctc
   106261 tatcgtccat aagctattac ccattcctct gcataaagtg attcataaag tcaaccagtg
   106321 ctgtaacacc ttcaacaggc atggcattgt agatagaagc acgcaaacct ccaacagatc
   106381 ggtgcccctt taagccactt aagccttcct tttctgcttc tttcacaaat ttttcttcaa
   106441 gttgttcatt tttcaaacgc cataccacat tcatctttga gcggctatgt ttatcaacag
   106501 gacaattata atatccattg cttgaatcaa tagcattgta cagtattgcg gctttttcgt
   106561 cgcggcgttt ttcaatttcc ggcaatcctc caattgattt aatccacctg agtgtaagtg
   106621 ttgtcatcca cacagtaaat gttggcggtg tattatacag cgaattttct tttgcatgca
   106681 ggtcatagcg taaatatgct ggtacatctt ttcttgcttt ttctacaagt tcttttttca
   106741 taacaaccag tgccatgcct gctggagcca gatttttctg agtgcccgca aaaagcatgc
   106801 tcaccttatc ccactccact ggccttgaca tgatatccga tgacatatca acaattaatg
   106861 gaatattacc ggtagtgggc atcttttgcc actgaatgcc atcaatagtt tcattggaac
   106921 atatatgtac atactcagcc cctggtgtat actgtaactc tctctggtct ggaatgcgtc
   106981 ggtatttctc agaagcccca tcccagataa catgaggatt gccaatgagc tttgcatctt
   107041 catgcgcttt tcttgcccag gtacccgtta atataaaatc agcaatttta ccaggcccaa
   107101 ggaatgctat aggtatcata ccaaattgga gagtagcacc accctgtatg aacagaatat
   107161 agtaatcatc cggtacctgc aaaatctctt taataagagc tatcgcctca ttatgaactt
   107221 catcgtatat tttgccacgg tgactcattt caatgagaga cataccagta tgcctataat
   107281 caacaagttc cctgcttgct tcttccagtg ctggcaatgg taatattgaa gggcctgatg
   107341 caaaattata gatacgcgcc atgtattcta ttctccttat ggaagtataa ctattctaaa
   107401 agcggttatt acgtattaat accacattct acttgctaaa aaagctattg atatgatact
   107461 gttataaaaa atcaagcaaa taaatccaga gggatacccc atgtcaggaa aactgtatat
   107521 tattgcaaca ccaattggta atatggaaga tattacatta cgggctttac gtatccttaa
   107581 agaagaaact gatatagtct attgcgaaga tacacggcaa acagcaaagc ttcttaataa
   107641 ttacgatata catgtaccca tacgttcact gcatacccat tcaagcaaag ccagactaca
   107701 gagtattatt caggaattag agtcaggcaa aacaatagcc tacctcactg attcaggtac
   107761 cccaggaata tcagatccag gaggtcaact ggtagcactg gcacgaaagc acaatatttg
   107821 tgtaatccca gtaccagggg cttcggcagt aacagctatt atttcagtgt gcggttttcc
   107881 tgcaacacaa ttctattttg caggtttttt aagcaaaaag gaaggtaagc gaaaaaacca
   107941 gttacaaaaa cttagtcacc ttgatgctat tattgttttg tatgaatctc cctaccgcat
   108001 cataaaacta ttgcatgcaa ttgccgaagt tatgccacag gcacagattc tcattggcag
   108061 ggaaatgacc aaacattttg aagaattcat ttattgtaca gcacgtgaca ttgaagacat
   108121 tgctgcaacc ctaacacaaa aaggggaatt tgttgtggca gtgtataatg ctaaaaaaac
   108181 gtatatgtaa aagtggaagt ggatgattaa tattaaatta tatattattt tacattttta
   108241 tattgacagt ataaatttgt aaatatatag taaactcatg aaatattatc caagaacatt
   108301 agaaaaaacg ttagctcatt atttaaaact tttcccggct gttgcggtat gcggtccacg
   108361 gcaatccgga aaatccacca tgttaaagaa acaatatggt cataagtacc gctatatcac
   108421 atttgatgat ccaattgtta gggaacgttt taacattgat cccaaaggtt ttttacacga
   108481 atataataac caggtcatct ttgatgaagt tcaaaaagtg ccgggaattt ttgaatatct
   108541 taaaatgtta attgatgcaa atcgttctca atatggtaat tttatactta ccggatccag
   108601 ccaattttca atggttacac atataaccga atctttagcg ggtaggattg gagtcttaac
   108661 cctattgcct ttccaatttt cagagattcc gcaatcactt caaaagcatc agattatata
   108721 cggcagttat cctgaaaatg tagtgcgaaa ataccagggt gtacatgaat ggtatgctgc
   108781 atacattaca aattacattg aacgcgatgt ccgttcactt tttaatattg gaaacttaaa
   108841 agattttcaa cggttactat cgctactggc ctctcaaact gctcaggaac taaatatgaa
   108901 caggttagcc aatgacattg gtgtaaccgt aaaaacaatt aaatcatgga tttcagtact
   108961 cgaggcaagt tacatactat ttacaatatc accatattac aacaacatag gaaaacgaat
   109021 tgtaaaaaga ccaaagattt atttttacga tacaggcctt gtatgctacc tgaccggcat
   109081 gcatactgta gatatattac acaaagggcc cttagcggga ccaatgtttg aaaattatat
   109141 tattgctgaa ttaaaaaaat tactggtaca ctataataaa agaatgaatt tatattacct
   109201 cagagataat gcgagcaatg aaattgatgt aataattgaa gatccattaa acaggaaatt
   109261 gattttaacc gaaataaaac acactgcaac acctaaacca ctgatggcac aacggttgca
   109321 aaaattggta acgctggtaa aaaatcatat tccagcatat actgtacatg gcatgatact
   109381 gtaccaggga aaagaagatt tttacgtgca tgatgtgcgt tttgctaatt ataagcattt
   109441 tcttgatgaa ctagaaaggc atttttagtg tatatttgat tctctttttg ttttttgttc
   109501 tataataaat atgattaaca tcatcacgtt aatcattatt ctaccctgat attaatttaa
   109561 ttacttgatt aattcctcta ttaatgtata tttattacaa aatcatttaa gatttgacaa
   109621 ataatagccg atagttacta tagattaaaa gagggtgatt accatggtta ttgataaaat
   109681 aggaaacatt aagaatattg ttgaacctaa aagcaccaaa tcggttggta aaacaagatc
   109741 cactggtaaa agtgactcgg ttgagatttc aagtgaagct aaaaaggcag cagagcaatc
   109801 caaaattgcc caggttgtta aacaaacatc tgatataagg gcagaaagag tacgcgaaat
   109861 caaggaacag attgccaatg gcacatatga tttcaatgat gaccgtataa tagataaagt
   109921 tgcagaaaaa attgccatgc agcttttacg aaaataacta cacccttatt aaaggttatt
   109981 atagatggcc ggcataattg acaaaaaggc ggaatcagga ttccgctttt ttatgtctaa
   110041 agcctatgga caaactatag aaggcctgtg gccggatcta tacattccaa ctgcggtgta
   110101 tgcacaaccc gatagtgtaa cacttcttcc ttcaataatt aaactttttg gatcacgtat
   110161 attatttatt accagttcaa ttgattttaa ccagtataac caccttttta tgcccattgc
   110221 acggagcctg caaaacaata acataccgtt catggtatat gatgaaattc ctgaaaacaa
   110281 tactgattat attgatactg cggtgtattt tgcccgcaaa acccattgcg acctggtcat
   110341 aggatttggc ggtattaatt caatcaacgc tgcgaaagca gttgcactgt taacccctaa
   110401 ctatttcttt gcacatgaac ttttcaccta tccttttcta catgagccaa ttccatttat
   110461 cagcattcct gtacatccac tgtttggatt tgatataact ccctgtacaa tattgactga
   110521 tattcatgac ggtatccaca aaacattttt tcacaattac atcttcccta aagcagttat
   110581 ccttgacccc gctcttgcat ttggtattga tgatgaaact atggcccgat atagtatagc
   110641 aacattagct attgcaactg aatcagtcat ttcaagaaat atcaataata ttaccaatac
   110701 actttcgctg agaactatag acctcatatt taaaaattta cctgctgcat tgcgcgaaag
   110761 tgacaatgag actgcacggc ttaatgtgat gatggcttca ctaatggctg gtattgcatt
   110821 tagtagtgca cagctctctg tctgtcttgc tctgtcactg gcactttctt cattgacacc
   110881 aattgattta gcatcatata tggctgttat gctaccacat attatggaat ataatttaac
   110941 atcggcgccc gggaaatacg tccagatgtc aaaagttatg gatgaggatg tacgtgatat
   111001 caccgttatt gaagctgcaa ttaaagctgt ggaaggtgta agaaaattag ctcttgatgt
   111061 tgaaattccc caacggttat cccaatttga tataaataag ggcatttttt ccaaagtagc
   111121 tgaaattacc ttgagctacc catttataca taatgcaccg cgtgcactca accgcgacga
   111181 agttgaaacc attctcattg ctgcctatta gcttatgagt cagtctgcac ttaacaattt
   111241 actgcaacgg tttaaaaatg gtctggtatc cgaagaggat tttttacagg aactatcgca
   111301 cataacgata gaacagttag gccacaccac actggatcac cacagacata ccaggttagg
   111361 tttccccgaa gttatttttt gccagaacaa aacacctgag caggttgccg atattgctaa
   111421 agcgttagcc aaacgtaaca caagcttttt aggtacacgt gccacaaaag agcattacaa
   111481 caaagtaaga cgacacatta aaaaagccca ttataatgaa gcaggcaaga tcatttctta
   111541 ccgtgcacaa acacttcacc ataaaccaca taaactggta attataacag cggggacatc
   111601 ggacataccg gttgctgaag aagcaaaaga aaccgcactc attatggggc ttactgttga
   111661 aactatctat gatattggcg ttgctggtat tcaccgtgta tttcataact gggaattcat
   111721 tcatgacgca acagttatca ttgtgatagc tggtatggag ggagcgctgc caagcgttat
   111781 tggagggctt gtcccctgcc cggttattgc tgtaccaacc tcagtgggat acggtgcttc
   111841 atttggtggt atagcggcgc ttttggggat gctcaactca tgtgccccgg gggttagcgt
   111901 tgttaacatt gacaacgggt ttggcgctgt gtactgtgcc tatcgtatta tacggagttt
   111961 gtaggatagt tacatgaaaa tactttactg cgatatacag ttaggcgctt ccggtgatat
   112021 gcttgttggt gcacttattg atatgggact tcctgttgat gttattacca cacagctaca
   112081 atcattatca gtaaaaggtt ttgcgatagc ccctgcaaag gtagagcgtt atggaataca
   112141 cggcacacag gctggaatta ccgcacacac tgacaccaca caccgtaatc ttgatagcat
   112201 atataatatt attgcacata cgccatgctc tgacactgtt aaaaccaatg caatgaagat
   112261 atttgacaca ttagccaccg ctgaagctac agtacacggc atttcaaaag aagatgttca
   112321 ttttcatgag gttggagcca ttgacagcat tgtggatata ctttcttttt gtattggtat
   112381 tgaatacttt tcaattgata cactgtatta cagcacattt gcgcttggaa caggcactat
   112441 caacacacag catggcatgt tgccgttacc tgcacccgca acggtaaagc ttacccacgg
   112501 caaacaggtt atgttcactg gactgcaggg tgaactgacc acaccaacag ctgctgcaat
   112561 cctggtaaca cttggcacac aacagacaca atttggcggt actatagtaa ctacaggtac
   112621 cggttttggt acacgtgatt atggttttcc cagttatacg cgaactttgc ttattgagga
   112681 taattctaat gaatacgaag ttctgagtgt actggaatgc actattgatg acatgaatcc
   112741 cgaaatatat cctcatgtgg ccgagttgtt atttgaagcc ggtgcgcggg atgtatattt
   112801 gcagcaggtt attatgaaga aagggcgccc cggggtggtg atttcagtac tctgcccttt
   112861 tcatgcggtg gatcaatgta cacagattct tttcaggcag acaacaacgc tgggggtcag
   112921 agcatatacc gtcaaccgct attcgttgcc acgagagttt gcaacagtaa tcctgtacgg
   112981 agaggaaatc cgtattaagc aaagttaccg atatcaggaa ctatcgccaa aacctgaatt
   113041 tgaggattgc aaaaaagtag cattaaaaac gggaaagccc ttatatcata tcatggaaga
   113101 ggctctgaaa gaatactata aacagaaaaa attatagctg cttcaattcc tcagaagaga
   113161 agatctggtc aatatcaaga atgatgacaa acttgttttc aaccttccca ataccaacaa
   113221 taaatttgcc cgatatgcct ttcacaattg gtgggggtgg ctcaatatct ttgttttcca
   113281 gtttgattac atgacttacc tggtctattg ccagccctat acgcttacct tttatatcaa
   113341 caactattgc acgtttattt tttgcaccct tttcagatat accaaaacgc acacccagat
   113401 caataatggg tataaccttt ccacgtaaat caatgactcc cataatataa tcagccgatt
   113461 ttggcagtgg tgtaacagct ggcagtttaa ggatttcctg tactttaaga atttctatgc
   113521 catattcttc cttgccaata ttgaagcaca ctatctgcat actattaagg gctgtatctt
   113581 tagtctgggt tttcatactg aaatccttaa tgcaatttta actcattttc acaatagtag
   113641 taataataac tcaatttttc aagttaaaat ttttattttt tggcgatagc tcatgagaaa
   113701 tcattttatt aattttaaat acgattgatg caactggtca attgctattt tcccttcttc
   113761 tgcatatccc tttaaattag caggaagtga accgttttta atatatatgt atcctgcaaa
   113821 cattataaac agtactgcaa taatgacaac aagcttcata acctgcttta taagcatatg
   113881 aggctgggga ataatttcca aaaacagctc atccaattct ccatattcga ta
//
LOCUS       DAORYL010000004        87890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_209_length_87890_cov_12.413651, whole genome shotgun sequence.
ACCESSION   DAORYL010000004 DAORYL010000000
VERSION     DAORYL010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 87890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 87890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87890
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209_length_87890_cov_12.413651"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1201
                     /locus_tag="PLV81_01745"
                     /pseudo
     CDS             1..1201
                     /locus_tag="PLV81_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930031.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            1278..2852
                     /locus_tag="PLV81_01750"
     CDS             1278..2852
                     /locus_tag="PLV81_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HOM08858.1"
                     /translation="MMKSYIRLCYIILIIILSSCTRFESVRDPYTLYIHISAEPGHLN
                     PITSNEAIASSINSYIYETLLDRDYDTLELKPQLATRWEISSDKLHYRFYLKENIYWS
                     DGKPFTADDIVYSFKIIKDPRVANAPLKVYYIDVKSVKKISPYIVEFTYAKPYFLALE
                     ICGSIPIVPKHIFDDGTDFNTHKNNRFPIGTGPYKFVTWNTGKTIELVVNDRYWGPKP
                     DIKRIVYKIIPEPNVALQMLKKGELDVMALRPIQWVRQTNSKHFQESFYKFMYYQPYY
                     NYIGWNARNPLFKDKNVRKALTMLINRQAILDSLLFGLGTVVTGPFYIHDKYYNHDIQ
                     PLPYDPAKAKEILHRAGWSDSNGDGILDKNGVAFKFTLTIASASKFAERLATILKEDF
                     SKVGIQMDINRYEWAIFVNKVDKRDFDAVTLGWSLSWEGDPYQLWHSSQVQAGSNFCY
                     FINAEADEIIEKARKEFDVEKRIKMYRRFHEIIHDEQPYTFLYCTPALVVVSKRFDNV
                     IVHKRGLNYTEWKVKQ"
     gene            2855..3856
                     /locus_tag="PLV81_01755"
     CDS             2855..3856
                     /locus_tag="PLV81_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOM08859.1"
                     /translation="MKYYILKRILIIIPTFIGITFITYLMIRLAPGDYTSLRAGVEGE
                     LKAGALSKEIFEQEKKLYGLDKPVIVGYAEWFYKTVKLDFGTSRKDGRKVISHIADAL
                     PITLALNILSIIIVYIISIPLGIYSSIKKDTVQDRIISLTLFLLYSLPSFWVALLLLK
                     YLSGGDYLNLFPLGGLYSDWFDQLNLFQKAMDILWHLILPVVTLTYGGFAFLSRYTRA
                     TMLDVINQQYIITARAKGLSEQKVLFVHAFRNSLIPLLTLMATMLPGLLGGSVIVESI
                     FSIPGMGMLAFESILSRDIPIIMAITSISAFLTLIGIFLADIMYAIADPRIRLEAKQ"
     gene            3853..4818
                     /locus_tag="PLV81_01760"
     CDS             3853..4818
                     /locus_tag="PLV81_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOM08860.1"
                     /translation="MKGYWSIVWKRFKQNKLAVVGLCIVIFLFVIALCAPLIANNKPY
                     ILIYKDKLYFPILFEYPEFSGLDFKKIEGFKIFPPIPYSYSEYDLNAIVLPPSAKHIL
                     GTDEQGRDLAARMLYGTRVSIFVGFVAVFIYVSIGIIIGALAGFYGGWVDIIISRIIE
                     IVMCFPTFFLILTILALWGQSLLNVMIVIGITGWTGIARIVRGEFLKLREQDFVIASK
                     ALGARDYWIIFKHILPNAMAPVMVSATFGIASTILIESSLSFLGFGVQPPTPSWGDIL
                     SQSRDFIDFAWWLTLIPGFAIFITITSYNLVGEGLQDALDPKQYR"
     gene            4790..5683
                     /locus_tag="PLV81_01765"
     CDS             4790..5683
                     /locus_tag="PLV81_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08861.1"
                     /translation="MRLIQNNIAKIVLILFISNTIACLSFFEAKQETNDLLPGETELY
                     TWKLVKSITVTNNDAIGNISPDYYKYYNVMEIVKGDYQSINNSSFFLTVTIARTVTVD
                     DAYGLFTRERIYVEKPFYKNDYSIYWKNKSIAFKGNIFLKVETNDLDFIQTQKSIIET
                     ILQKIQNAHAIPDYVVRISKYCDIDSIILFNEGIPQIPYLKNIVVGKKIIESKDIMVF
                     FKIYSTNIDASQNFDAILKNDSTLMILESGKVKIAFKKCGDSYCYIAQYKEWIFGLYD
                     IVDYNAGKQYINTLYKELSNP"
     gene            5684..>6207
                     /locus_tag="PLV81_01770"
     CDS             5684..>6207
                     /locus_tag="PLV81_01770"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOM08862.1"
                     /translation="MFYIIGDIHGYYNKLSGLFEKIKKHITDNDTIIFLGDYIDRGKH
                     SFEVIEFLIDLQQSYNTVYLRGNHESMFLDYLQGKEIPLYMYNGGQATIDSYIQMMGS
                     LRLPEDHKAFFNSLKLYYETDDFIAIHAGMRPGIPVELQSPYDMMWIREDFYLKQYRW
                     PKLVVFGHTTTVNIT"
     assembly_gap    6208..6307
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6438..8660
                     /locus_tag="PLV81_01775"
     CDS             6438..8660
                     /locus_tag="PLV81_01775"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOM08863.1"
                     /translation="MNIFEWLNKKFSYTFALCFFTIFGGWMSAVFGYYLGTSFILSEY
                     NEMHSLAIKWLPLAVLIPTLLHYITFGFLTPLGIPAFFKSLRDVNNAFKGGTLNPSLT
                     DENLQVLYIQLSHLPMYNMVAASLFGTLCGFFLMGFGYYDMITHGTFTIIKMKIGIKI
                     VTIGVLVVVVLYGMSTYLLSEIITNPHRALVYQELRQRTINIYPRGLIGLRIKFSFFI
                     ILMIITLLTFAAMMEQHRLYEETRYINILTYFLVSIVAGVFLMYINSDSVMRILDEMG
                     IVTKRISSGQEAWFRVMSYEREFAEIEFSILEMAKEIEEHRKNLELKVEQRTEELREA
                     LASLKEKDDMIQKQLEIASNIQRSILPGRIDDWNELKFSVRYIAMEKIGGDFYDVYQM
                     KGDKLGILVADVSGHGIPAALITSMAKISFSSATQQYDSPKRVFQEVNQNILEHIKTQ
                     DYMTAFFVVIDDDYNVTYANASHQKAILIRSDEGRTELLDTNGLFIGAIEEARETYEE
                     KETKLKYGDRLLLYTDGIPEAANIEREEYSVERLQEVAVKNRHLSLEDFTSFIIEDVQ
                     RFKGKAPVEDDITLLVIELARDEAVDIIKQAKKLIDEHKYYEAIDYLERSLALYPNNR
                     KLIYNLAKNYFRVNNFGKATELIEQYLEMDKRNKYAYYVGGAAYYQMMNYEKAIQLLE
                     KAQSLDPNFTNALFALGMAYKKIGMYQDALQIFERVANIDPDNKMALFEINEIRKGLA
                     "
     gene            complement(8670..9329)
                     /locus_tag="PLV81_01780"
     CDS             complement(8670..9329)
                     /locus_tag="PLV81_01780"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOM08864.1"
                     /translation="MKSYIILFLKIISKVVIVIFVTCIILNDLISFHANAQIKSKKTD
                     DNTIEYYNLQHNKKTKITKVDLSKNPYDTIIINICKIENIDPQLIRCIIKVESNFNKD
                     AVSVSGAMGLMQLMQETAQAYNVQDPFDPHQNIQAGVKHFKSLLSVFKNDIVLALAAY
                     HAGLGIVRKNMAVPNIKSTITYVNDVMKLYQPDENYNYSAKIEKLYMHIMPDGTINIT
                     Q"
     gene            complement(9292..10746)
                     /locus_tag="PLV81_01785"
     CDS             complement(9292..10746)
                     /locus_tag="PLV81_01785"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOM08865.1"
                     /translation="MNIVFITSEAYPFAKTGGLADYSYSLPKALAKRRHTVHLFLPRY
                     YCVDKQRFNCRLIGKPLAVPIANGIRWAGIFYTDYLEGVHTYFIEHDDYYGRDGLYDY
                     NGVSYNDNAERFIYFCRACIEAIKQLAIVPEIIHCNDWQTACIPLFLNTHYANDYVLK
                     KAKSILTIHNVGYQGVFGKEVLGLMQLSEELFNPENIEFFGKVNLLKAGLVNADNLVT
                     VSKKYAQEIQESEYGYDLAPIFKKYNQKLSGILNGVDYEHWNPETDDFIPYKYSIHNL
                     TGKQKCKKALKNFYGLKNDNVPIIGCISRLTYQKGIDILINTLDWLFFDEKDIQFVIV
                     GSGEQWIVDRFEALRAMFPDRIGVWWGYNEKLAHLIEAGSDFYVMPSRYEPSGLNQMY
                     SLAYGTIPIVRATGGLDDSIEQFDERLKTGNGFKYKNNDSAELYTTIRHALRVYKNKV
                     IMNKLINNAMLFKRSWDDTAKEYEELYNTISQNN"
     gene            complement(10748..12400)
                     /gene="gpmI"
                     /locus_tag="PLV81_01790"
     CDS             complement(10748..12400)
                     /gene="gpmI"
                     /locus_tag="PLV81_01790"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOM08866.1"
                     /translation="MKLLPNGFSRRKGPLVLIIMDGIGIGPHDEGNAFFLARTPVLDK
                     LMQTCPYTTLKAHGTAVGLPSDADMGNSEVGHNALGAGRIFDQGAKLVDKAIESKQIF
                     STEIWHKLLEKPVNTGTTLHFIGLLSDGNVHSHINHLFKLIEQAAKEGVKKIRVHILL
                     DGRDVPETSALQYVDSLEKFLHTFNQNGFDFCIASGGGRMVTTMDRYEADWNIVKRGW
                     DVHVLGKGRAFTSATEAIETYRKEQPGITDQYLPSFVIAKDGKPVGPIIDGDSVILYN
                     FRGDRAIEISRAFEEKDFNKFDRERFPDIVFAGMMEYDGDLHIPKNYLVFPPSIDKTI
                     SEYLVHNGCRQFAISETQKFGHVTYFWNGNRSGMFDQNYETYQEIPSDRVQFNERPWM
                     KAAEITDAVIDAINSNKYDFIRLNYANGDMVGHTGILEAAIIAAETVDLCIGRLLHAI
                     DNTGAIAIITADHGNLDEMYEKDAKTGTIKIDKKTGKPQPKTSHTLNPVPFIIYDSQY
                     NGEYTLTTHKEQGLANVAATICTLLGFHSPEEYYPSLVSLTK"
     gene            complement(12401..12703)
                     /locus_tag="PLV81_01795"
     CDS             complement(12401..12703)
                     /locus_tag="PLV81_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOM08867.1"
                     /translation="MIFTKKEEENRCIFIISEQLNPRTLKELIEEMHNFLQNDSRDAI
                     IDMTNVEVVDSTILAGFMTLYNNFNNNRRKFRIINANNYVKRVIELASLETFLLEE"
     gene            complement(12715..13056)
                     /locus_tag="PLV81_01800"
     CDS             complement(12715..13056)
                     /locus_tag="PLV81_01800"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOM08868.1"
                     /translation="MNFTHTRYSDLIIVTVTGIGEYGDDVKLIKLLEKQIPEKISTVA
                     INFSQVDTINSAAVAAIMYLLKYGDEHNFDVVFYAANDKIYMILERALTKYSINIFTE
                     DEFYKKYNITV"
     gene            complement(13053..13763)
                     /locus_tag="PLV81_01805"
     CDS             complement(13053..13763)
                     /locus_tag="PLV81_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08869.1"
                     /translation="MIKLTKDKIFIFDKKLFIILISLFVLLYIFNTIPLYAIEIFDTY
                     NQIKTTFDGLSRFLDNIYSFISAISNLLGYRAIALFFAIAFTSAGLSAIGFPKGKTSF
                     FISMIIINILWFTWNSSFEIDYLYTISTMLKTNGIILFPYILFLIIKYYKEVIKRFMY
                     TCLYLIFPFLKKEGIRKQTLQESVKLINTTYTELIEYLMKDYFYGDDFIKLSDNSIRA
                     IEQLENILKKIKESGRSK"
     gene            14003..14752
                     /locus_tag="PLV81_01810"
     CDS             14003..14752
                     /locus_tag="PLV81_01810"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOM08870.1"
                     /translation="MDDLIKTAAKIVKDSISTIALTGAGISVESGIPDFRSAGGLWTR
                     YNPEEYAHIDGFRRNPRKIWKMIFELMDMTRNAMPNPAHIALAQLEEMGLLKAVITQN
                     IDNLHQRAGSKNVIEFHGNSERLQCIYCGAEYAPDEFVIENEPPQCKKCGEILKPSVV
                     FFGEMIPHNALMESQNLASDARVVLVIGTSAVVYPASSIPHIAKANGAYIIEFNLEKT
                     VITPMITDIFIQGKVGETLPKFIEAIKALQA"
     gene            14756..16498
                     /gene="mutL"
                     /locus_tag="PLV81_01815"
     CDS             14756..16498
                     /gene="mutL"
                     /locus_tag="PLV81_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOM08871.1"
                     /translation="MELSPNIQQRIQILPDAVKRKIAAGEVIDGPYSVIKELVENALD
                     AQATDIDIEIEESGLKRIVVRDNGIGIYRDDLPLAVEEHATSKIKSIDDIFSIITMGF
                     RGEALSSIAEVSHCTILSRTAEEEIGGKLVIDEGKKELFDWAGPTGTTIIVENLFFNT
                     PARKKFIKGLKSELNRIKDTIFALAIANSRIQFKVKIDNKIAFVFEPADTLQRIAQIY
                     GNEMADNLLYGSLSDLHCTIEGYCTKPNYYRSNRSLQFLYVNHRPVQFPYFNFILQQV
                     YQTILQKGQYPAAFIFCNIKPDLIDVNVHPAKKEIRFFDNNYIHSMIYNFANKLISGT
                     IHAFPIKHNDTQLQNNNITTASNANNYTDNNTPPLLYTQPNVNPQTLIHEVQHLYTQL
                     HNKDYSVIGTIFGSYVLIQKENNLYIIDFHAAHEKKLFDYLMSIDSVSAVQQLIMPVV
                     MELSPDLLIHIHSYKDILTNYGIIIDQFDEHSIVINAIPDFCNTNDIEGLMRDIVDSL
                     HENKYSLNNIKIKIIEKIACHSAKRANDIITQDDIIAIADYVLSTNDYRCPHGRPFVY
                     IVSKSELERFFKRS"
     gene            16495..17031
                     /gene="coaBC"
                     /locus_tag="PLV81_01820"
     CDS             16495..17031
                     /gene="coaBC"
                     /locus_tag="PLV81_01820"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016753055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOM08872.1"
                     /translation="MKQKIILGISSSIAAYKACDLARLLTKDGYDVFCVLTENATHLV
                     APLTLQTLTGNPVYTTSFAIQWREMGHIELKENAALLLVAPATANIIGKFANGIADDL
                     LSTTFLAVNCPVVIAPAMNPNMWKHKAVQENIKKLKEWGCIFVEPETGLVACGDEGQG
                     RLASIEEIYKVAINAITR"
     gene            17015..17683
                     /locus_tag="PLV81_01825"
     CDS             17015..17683
                     /locus_tag="PLV81_01825"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HOM08873.1"
                     /translation="MQLQDKKIIVTGGPTREWIDPVRYISNASSGKMGIAIADAAYKQ
                     CKELVFIHGPIDESLISGKPYRCKGVESTVDMLEAIKQELSPNMVLIMAAAPADYKPV
                     TKSPTKIKKTNENLTIELQRNPDILKTIASIKKQQQEFNNIVLIGFAAETTDVIAYGK
                     TKLVEKELAMICINDVSRSDTGFGSDFNEVTVMLANGMIINLHRMTKKEVAEGIINII
                     INEL"
     gene            complement(17678..18370)
                     /locus_tag="PLV81_01830"
     CDS             complement(17678..18370)
                     /locus_tag="PLV81_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOM08874.1"
                     /translation="MNIINTDSIGYQYPDGTVALHNISLIVSENQKVAIIGHNGSGKS
                     TLLLLLSALLLPTSGTITIFDMQLTARNKSQVRKSIGIVFSQVEYQFIMPDLLNDVIL
                     SIREGSQEEKKSQALQWLKKVNMDDMLNHSPLALSSGQMKRAALVSVLAKNPKILLLD
                     EPLANLDKPSADAVIHILSDLNMPMIFATHSAYAVQHLANRLIVLEHGTIAYDGVCNS
                     KILEKHKKTILL"
     gene            complement(18377..18919)
                     /locus_tag="PLV81_01835"
     CDS             complement(18377..18919)
                     /locus_tag="PLV81_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08875.1"
                     /translation="MYIPKQQRYTLVMIIPLVITIAVMQLLTGLMNEQLDFVIISITA
                     FKIVCITIIIIGSRFFIGSDGFKAFINILPYSLKLFFLIFIRILYTLLKLNRMIVFQI
                     QSRIDLKTKDKYYIPKYYSIAFFSNQFYALAYYRNGILSRTISTIPNLIIHNKHSINE
                     YISSGVIIILLIVNCIYQGL"
     gene            complement(19042..19662)
                     /locus_tag="PLV81_01840"
     CDS             complement(19042..19662)
                     /locus_tag="PLV81_01840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOM08876.1"
                     /translation="MVNKNYDDKIEYFNTLSKNWDEIVGNDETRIQKIKNIFGMIDIT
                     YGSRVMDVGCGTGVLFPIIEEYIGPQGTLIAVDTSDKMIEEAKERYKHFTNIQYIVAP
                     LEDITLPDNSIDVVLSFAVFPHIEDKLKALKKCHECLKNNGSLYIFHLSNTKTLNEFH
                     HNLNAPVSRDYMPYREELDTMLREAGFIMKKYIDQPELNFIHATVE"
     gene            complement(19665..20189)
                     /locus_tag="PLV81_01845"
     CDS             complement(19665..20189)
                     /locus_tag="PLV81_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08877.1"
                     /translation="MNDELHITQIIISAITAACAVVIPVLFHLLGLGSIFLPMFIPLS
                     IGAYFLTPAFALLTGTIAPITSTVLTGMPPLYPPIAIIMMIELSLFCFFISLLKQKFY
                     LNNYLIIVFAIIFDRIILYVLYAIVLPYFKISFGMFSLYDLFKSFPGIILLILITPTA
                     VNGINVIIQKNAGR"
     gene            20569..22284
                     /locus_tag="PLV81_01850"
     CDS             20569..22284
                     /locus_tag="PLV81_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOM08878.1"
                     /translation="MAKKFIPDWTEKAPEPYSYRSIFKWGNPETFKHPNSKLYQELKQ
                     IFQLDDSYFQHKTNEGRQQVQYKKPVNLTQNQIKALAEIVGYDNISTNEYDRLKFATG
                     KTLEEALKLRKGIIERVADCVVHPRNVEDVQKIVAYCNKHKIPIYVYGGGSSVNFGLR
                     PAKGGLTLVLSTHMNKILNVNETNQTVTVQAGILGPALEEALNNAQKLFGTKYNYTCG
                     HFPQSFEYSSVGGWIVTLGSGQSSSYYGDAYDLVISMKWVTPVGTFTTKDYAATATGP
                     KVNDIMKGCEGTFGVLVEVTWKIFRYMPENRKRFGFIFKTFEDAINASREISQGEFGM
                     PAVFRISDEEETHIGLKLYGIDGTILDTFMQLRGYKPMKRCLFIGSAEGEKHFAKNIK
                     KQVKKICKKHGGMYITGLPTRMWEPGRFKDPYLREDLNDYGILIDTLETSVKWDNLHH
                     VHKAVRQFIKSRPQTVCMTHASHFYPQGTNLYFIYIMKTDDINEYIQFQDGIIDTIAK
                     SGGSLSHHHGVGRMLAPWMEEHIGKEQMAVLRAIKKHFDPNNVMNPGGQLGLDLKDKN
                     WRKIK"
     gene            complement(22344..23606)
                     /locus_tag="PLV81_01855"
                     /pseudo
     CDS             complement(22344..23606)
                     /locus_tag="PLV81_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5982 domain-containing protein"
     gene            complement(23943..24701)
                     /locus_tag="PLV81_01860"
     CDS             complement(23943..24701)
                     /locus_tag="PLV81_01860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOM08879.1"
                     /translation="MNPPLTKKSELALYDEGYYTGKANYTYYDERDVEHFSQYVWNAR
                     LKTIRKYVQSGNLLDVGCSFGGFLQSASRYFTVYGIEPSEYSGKYAQQRFSNDVIHVG
                     TLFDHPFKEDTFSVITMIEVLEHIRNPREALTECYHLCKDKGLLVIQTANMAGLQAKL
                     QKQKYGYFLPGHFSYFTKKNLTETLTQIGFRHVTVYQPVDFGLLPKLLKSRMNFKSIN
                     DYKNWLRIATYHYISKIHIGNLALTSSMVVYAFK"
     gene            complement(24839..25474)
                     /locus_tag="PLV81_01865"
     CDS             complement(24839..25474)
                     /locus_tag="PLV81_01865"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOM08880.1"
                     /translation="MDATTQSWNKHYTRDTSVLLYPDEQLVRMLAKYAGNKKRALDVG
                     CGTGRHVKLLHEFGCDYVVGIDISFNALAHCNKILQGSFVQANTLKLPFSERTFDIVV
                     SWGSLHYTTKDNLPVMLQEIKRILCPGGVFFGTLRSINDTFIAKGRHLGNNTWETALP
                     DINGAVVSLFSLDEVKTYLHVFNEYHIGLMERTVLDDLTKTLSHWYFYATI"
     gene            complement(25477..26634)
                     /locus_tag="PLV81_01870"
     CDS             complement(25477..26634)
                     /locus_tag="PLV81_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOM08881.1"
                     /translation="MFIVSIGAGKNQIPLIKAIKELGYSIIGIDKNPLAEGFQYCDIK
                     VIESIYNYHDIYLLLKEQLVFDDIAAVVTRSYGQAIKTVAYLSQQFKLPYIDFNTVDD
                     LIDKSSFRTIAAIHNIPMPHGFIIHKKDLHKLNTLSFPCIIKPPKGHAKQSVMIIDSY
                     HELCSYMKSVKQDTVLIEEYIEGDEIIVLGFVHNRTFYIYDISDKILNAKPYFVDRMH
                     VLPSQYYTMYPELQKLGQKITDTFALQATPLLMECIIRNNSIYLIEAACEFGGEYLAD
                     YAIPARLKSNVFKLFLQAIITGKVNVPPKSSVSAIVKYIMGRNGKLVSYSMPKNNHLI
                     HAEMFAKPGDILYYPKNNHERLGVIITKGKTVEKALQTADTLLEQMHIIIE"
     assembly_gap    26645..26744
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(26886..27389)
                     /locus_tag="PLV81_01875"
     CDS             complement(26886..27389)
                     /locus_tag="PLV81_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08882.1"
                     /translation="MPQKPTLLHSHNDYWCKRPLWDAIDYGCNMIEGDIIYINKKLVL
                     SHSWRPFSWMCYGELENTYLRPIHQYCIENPQKEIWMYVEYKDSNEKINEILYALFRQ
                     YTISNLHYTISAQNEKWYHKKRYKTAMKFYEKYKEELQLTWKTTELAEQYEIKKVDLF
                     PESIWHF"
     gene            complement(27479..28561)
                     /locus_tag="PLV81_01880"
     CDS             complement(27479..28561)
                     /locus_tag="PLV81_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08883.1"
                     /translation="MQFIKIIYIALFFNFFCIAFLGAQTPDKQKIIIYYFNDESATQN
                     YRYYSYIIPDSLSAELNEKVEYEVTVLPVTFKYITSQVTDEEKANTIAILTSRGKEVS
                     ANFSVIGSYKVEKNTIHIKTQICNIATGDIVDAAETNEKIGALIYEIIDRITVKINKE
                     LQKTIEVEKEKIAVSPYKNFYSSLKNISLCYSYNFIFFTGDWEKLYNDTSMSKISIGV
                     PFSKIFTSNSAFINKLYLAPTWQLFSTNNRDLEHTYDTYLSIYMGGVSLSYIHTLSPL
                     IDVAIAIQPGIAYSDLYIVLVQEGGPFAPYVKKNSYDPFLSVEINAIFYFYSIGFTGG
                     FNYNRLFYKGNDLTATGINVGILYKL"
     gene            complement(28639..29835)
                     /gene="argJ"
                     /locus_tag="PLV81_01885"
     CDS             complement(28639..29835)
                     /gene="argJ"
                     /locus_tag="PLV81_01885"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOM08884.1"
                     /translation="MSTIQELAGGLENVSLYKYAAIECGIRYSNRLDLSLIYSSEPCW
                     AAGVFTTNKMCAAPVILCRERINNSINAILINATNANAATGKQGLENAKLLTRELSKL
                     LSIDENSVLMASTGIIGVQLPVDKMKNSLPVLVKNLSIEAGTLLPRAIMTTDTFPKQY
                     AISFKTSTGTYTMAGIAKGAGMIAPNMATLLCFVVTDFPVEQHILQKLFYESVNVSLN
                     AITIDGDMSTNDTAIILSTAKPSVHNVDDIKTFNHALHLMMKKLAYLIVSDAEGATKC
                     VTVKVIHAANDGDAKKAAKSIAESLLVKTAMFGNDPNWGRIACAAGYSGAELLEDKLS
                     IYFDEIPLFKDGSPVEYDKEKLMIILKKREYTITVDFGIGNSEFEFLTSDLSYDYVKI
                     NAEYST"
     gene            complement(29874..30617)
                     /locus_tag="PLV81_01890"
     CDS             complement(29874..30617)
                     /locus_tag="PLV81_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007277193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOM08885.1"
                     /translation="MRPEKAYKNLDFLNSRDARIIRIMCEYIEPEKRFKHYNIKHTVV
                     FFGSARTKPDDPVTGKYYHDATRLAFLLAQYGKELEAVHDGFVICTGGGPGIMEAANK
                     GAKEAGTYSIGLNISLPFEQMPNPYISPELNFEFHYFFMRKLWFLYHAKAIVVFPGGF
                     GTLDELFETLTLVQTNKLQKHDIPIILYDKSFWDKFINFQLLVDYGYIDKHDLELIHY
                     CNTPEEGFEYLKPKLKELIERVNHTIENQ"
     gene            complement(30617..31204)
                     /locus_tag="PLV81_01895"
     CDS             complement(30617..31204)
                     /locus_tag="PLV81_01895"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM08886.1"
                     /translation="MSYSNNNNDVKKYIDSYNATSQTVPMVIDRLLTDLNEMQYPKEE
                     IEEIILSMDEALTNAIQETIRKEHNLNISYDPEMREITVMYIVSKDEFEAVIIDHGKG
                     FNFNESIKKTPDKTSFDYFEQITRYSSEKETGLHIRVNGQQISLKGIGAGLKIICAFM
                     DSINIDLIDKEKIVSDTVSPLTDGTILTMRRKRRY"
     gene            31647..33248
                     /locus_tag="PLV81_01900"
     CDS             31647..33248
                     /locus_tag="PLV81_01900"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HOM08887.1"
                     /translation="MKIFCTLLISIISFMGCSCGKYYSEAKQIVDDLNMESLLGQMLI
                     IAVPSNKIDEKTITIIKNYRPGGVILFGYNLNNGSCKSLAHDLQKLAMESIGIPLFIS
                     IDQEGGRVKRIQSGITQFPGNFALGVVNDPSKTYTMAQILGIQLRLSGINMNYGPVVD
                     VNNNPDNPVINIRSFGCEPALCASLGGAYIEGLQNSRCIAVAKHFPGHGDTYQDSHLT
                     LPVIYKSLSQLENVEFVPFKQAIDAGVECIMTAHIAFPRLGINIPATMSSFFLNDILR
                     KSLKFKGLIITDDLEMKGISGIQSYGNAAVNSIKAGADILLISSYDNHVQEIIDSLKN
                     ALANGDISVELIKSKATKILELKLRYNIMQIKDGKLLLKNYEPEPDDLKLLQQADALN
                     TELSQSSIVFYSKNTIHDFVIDNRNTYAEVTSKNDNLISIFKKENVIMDFPASNKKNK
                     ILLIDASSYSITELHILLKSLQSYNKVLLLITGNPYPYIKHFGNYSMLMSFSDTDESL
                     RQLALCLKGVFEPKISHPCLPGNTQ"
     gene            33245..34387
                     /gene="hemW"
                     /locus_tag="PLV81_01905"
     CDS             33245..34387
                     /gene="hemW"
                     /locus_tag="PLV81_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOM08888.1"
                     /translation="MNLGIYIHIPFCHKEKCDYCDFYSIPVKKNKSWHTLIEKYIKTL
                     CAEIIYYSTEFQDHVVDTIYFGGGTPSLLERNHYATIVNTIKNYYAIALDIEITLECN
                     PDHYSLNYIKELQSVGINRFVLGVQTLDKRAHEYIGRKPKPAGREIIEEFCSIPDIIS
                     CIDIIVGIPKQTVTSYIYSLNTIIQCKPKHISAYILTFEKNTVLSSRVPHSEKFSNFQ
                     RTMFEKTITILTTSGYRHYEVSNFALPQFESRHNCKYWNFEEYIGFGAGAHSFYKQRR
                     YYNSNLNDYLNNPIDSRIEDVRTIKDIAIEYLMNKLRLIDGFFLNELYIKTNYVPSSG
                     FKDAIEILIEKKLLQRSIIEGQDKLQCTYEGMFMLDSVLFELMNSI"
     gene            complement(34384..35235)
                     /locus_tag="PLV81_01910"
     CDS             complement(34384..35235)
                     /locus_tag="PLV81_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08889.1"
                     /translation="MSEIDEFSFDDDLFKLDDEIEEKKQHGTAKNRKVDDDTEDFKPE
                     KDSSDSIRKRSSRDFQPDMDALLITAQSSMIIEGMKKYTERDFSSGNLPIYLEALNGV
                     NLYIKILDRNPKNYHKLKAVINADPDCQEVEKIAFNLYKKIFGNEPETDQEKLVAFEK
                     FSALFKDAVNRATIATSMKDIKKYFLLSGGLDEIKVSQLVASNDDELKRDISNLQRYI
                     ALGLAYIEKGNVEIIKGLRGRDLNNYVVRGSQFLAYYFTLINNERLADYYARINTIYS
                     KYFVVKD"
     gene            complement(35324..36091)
                     /locus_tag="PLV81_01915"
     CDS             complement(35324..36091)
                     /locus_tag="PLV81_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOM08890.1"
                     /translation="MKGKLITALFLTCVAVTSLIFFVVALLIRIFTYPFDKRLRLLHL
                     LTCFWGSLYTYVMPPWKIKVEGRNKIRNNVPYVFVSNHQSQLDILVAFRLYKHFKWVS
                     KAEIFKVPVIGWNMFLNRYVRLFRGVKESSVQMLQDCEMHLKEGSPVFIFPEGTRSPD
                     GHLKEFKMGAFILAKNMNVPIMPVIIEGTRFALPKYSIDYHGKHKITLRVLDEITPET
                     YKDMTVEQLAQYTWNYMNNELRKLRIEMYGHDDVKNN"
     gene            complement(36382..37335)
                     /locus_tag="PLV81_01920"
     CDS             complement(36382..37335)
                     /locus_tag="PLV81_01920"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOM08891.1"
                     /translation="MSILIIADVKSRTINPSTLEMLSAAKKIAKNNTMPIYILIPFGN
                     IGGDSYCSSEFKTFILDLNIEQYSAHVLVKYISSVIREVNASYIIGMHTPFSIDYIPA
                     LSVSINAQCITQIHSLDVAERITVTRGSHAGKLDRHIVINDTPIILTVLPGSFEPYTI
                     TDVQTQHITTIHIPEVNKHIYCNFITKDMKDTSLDEADVIVGAGKGIGSRENLNLIYE
                     FAKVFPHSAVAGSRIVCDYGWLPYSKQIGITGKSIAPKVYIACAISGSSQHVAGIKGA
                     KTIIAINKDPYAPIFNVSHYGVVADIFEFIPQFIDFVQNNA"
     gene            complement(37342..38109)
                     /locus_tag="PLV81_01925"
     CDS             complement(37342..38109)
                     /locus_tag="PLV81_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOM08892.1"
                     /translation="MENKFKILVCIKQVPDTNALVYLNDGIPEYDEKTVYVINRYDEY
                     ALEEALQLKDCGLTSYIHAVSVGPHRLQKALRRSLEMGVDEAFHIQSDDDRLHNPYVI
                     AQAITQFAQQNYDIIFTGVMSEDTMNQQTGQLIAAMLGYKHATGVTELSLNNTTITIT
                     RELDAQESLMYELHIPCVISVQSSIHKPRYPSLSNVLRARKQEIAIIKQDLITSTGKT
                     KSYFTMPQKTTQCQFIEGSISQKVTELYTILHKNAVL"
     gene            38366..39085
                     /locus_tag="PLV81_01930"
     CDS             38366..39085
                     /locus_tag="PLV81_01930"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOM08893.1"
                     /translation="MSEILVIRHGQGSFGQGDYDNLSEKGYNQSQILAEYLYNYRYKN
                     KKIDALYCGTLKRQQQTAQTIIKYFELKGVQLPEPKIDRRLNEYDSITIIKTLFPVLL
                     DDEPSLEEEIKQIYTDKKAFQRVFEKVMRYWVKSQTNYEGIESFSEVKQRVKSAITEI
                     IQENGRGKNIIVVSSGGIIGAIVQMVLGFDDETAMRLTWQIVNTSLTHLRYNEKELIL
                     ASFNFFPHIELADDPSLKTYR"
     gene            39109..40317
                     /locus_tag="PLV81_01935"
     CDS             39109..40317
                     /locus_tag="PLV81_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOM08894.1"
                     /translation="MDFEISDKMKAILELIDEFVEKELIPLEQEFARNDIKAMLPVLE
                     EKRKMVKKMELWAPQVPKEYGGMGLDLVEHGLVSESLGRTPLGHYVFGCQAPDAGNIE
                     ILIKYANDEQREKYLLPLVRGEIRSCFSMTEVDLPGSNPVLLDTTAVKDGDYYVINGH
                     KWYSTAADGSKFSIVMAVTNPEAPTYLRASMIIVPTDTPGFNLVRNIPVMGHEGSDYF
                     SHGEILLQNCRVPVTNLLGPEGQGFVIAQERLGPGRIHHCMRWLGICKRAFDLMCARA
                     NKRIISPDGKTLATRQIIQEWVAECAAEIQAARLMTLQAAWKIEHLGQKEARIDVSLI
                     KFYVANVMQKVIDRALQVHGGLGMTDDTILAFFYRHERAARIYDGADEVHKSVVAKRI
                     LSSYEGREVR"
     gene            40295..41020
                     /locus_tag="PLV81_01940"
     CDS             40295..41020
                     /locus_tag="PLV81_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOM08895.1"
                     /translation="MKAEKSDSIHIVDLENFNSFKKSIKFILDDIVSKLYDDNKLLIR
                     VKKSPQSIENLKRIIHTAIAISNKKGYAVMSIRELSAVSSLSIGALYAYFPSKDELLH
                     IIQQYGRSSVEKVLSETLVHTSSSKDKLYNFIRAHIFISEVLKDWFYFSYMETRYFTG
                     SEYNKTIEGELLTESLIKDIIENGIKNNEFRSDIQASLIASISKAMLQDWYLKRWKYT
                     KRGVTPDEYALECFKILYNYIKL"
     gene            41039..42109
                     /locus_tag="PLV81_01945"
     CDS             41039..42109
                     /locus_tag="PLV81_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HOM08896.1"
                     /translation="MDNYIDKPTDIREGETIDGKIVLDYLIQHVPDIKGPIEIKQFPS
                     GFSNLTYFIKAGDIEMVLRRPPFGKKAKTAHDMHREYSILKALKPVFPYCPTPLVYCD
                     DESVIGAKFYVMERIKGIILRKELPPGLSYTPEEFRYLCEQLLDIQCQLHSLNYKEIG
                     LENFGKPEGYIERQVNGWIARYKDALTPDAPLYTEVIQWLQEKMPKTPTRAAVIHNDY
                     KFDNVVLDINDPKRIIGVLDWEMATIGDPVMDLGNSLAYWVEKNDPEEVHLIRLMPTH
                     LDGALTRKELVERYQEKSGIDMSDFDYFFCFGLFRLAVIAQQIYYRFYHGQTKDKRFA
                     MLIVAVQVLERQARKVIDNSKL"
     gene            complement(42106..42855)
                     /locus_tag="PLV81_01950"
     CDS             complement(42106..42855)
                     /locus_tag="PLV81_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOM08897.1"
                     /translation="MRIIAISNNKGGVGKTTSTVNIAAALQILGKKVLVIDIDHQAQS
                     TYHFGYNPNNLTYSVYNVLKGEIAYKDVIIDRDGLHILPSKNELKDIEFIPLPAKEFL
                     LKEALEGISGYDFILIDCPPSLGILTLNALTFAHEILVPLQVQFLPFHGMYNLFEAVQ
                     MVKRRLNKEVEITGIIGTMFNPKKQINVEVIEETKKRLPGKLFETLIRENVLLQEAPS
                     WGKTIFEYKPNSNGAVDYMNLTKEILEKDVN"
     gene            43188..44066
                     /locus_tag="PLV81_01955"
     CDS             43188..44066
                     /locus_tag="PLV81_01955"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOM08898.1"
                     /translation="MGNPAWASLMSDLIPAHKRGKYFGFRNSILGVLLVLVSLSVGIT
                     LRFSPAEYIMQTFFVVFLIASISRFISFYYLLKMHEPPFEIEKASIVPANIFNSIKKN
                     DFLIFIAFMSLMNFAVYLSAPFFAVLMLQHLSFSYFEYSLVTVSAPFIMYIAMHSWGN
                     IADKIGNAKVLTITSPLIAIVPILWVLNSHIVYLLIVQIFSGLVWSGFTISSFNYMFD
                     ASVSSKRVRYISYLNLINGIMISAGAVSGGYLIQILPPVNGSSIYTMLLVSGILRLSI
                     TIVFGKRLKEMRLVKI"
     gene            44286..44486
                     /locus_tag="PLV81_01960"
     CDS             44286..44486
                     /locus_tag="PLV81_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOM08899.1"
                     /translation="MPKGTIKWFNEKKGFGFISKEDGSDVFVHYTGIIGNGFRTLKEG
                     QKVEFEIESSDKGPRAVSVRAI"
     gene            complement(44542..45201)
                     /locus_tag="PLV81_01965"
     CDS             complement(44542..45201)
                     /locus_tag="PLV81_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOM08900.1"
                     /translation="MKKAERMQWEKEQKVNRFIQIAQGIFFQKGYDTTTIDEVAQRAG
                     YNKRTLYHYFKDKEELFLAVVLDALKLFNKKLQEAYDNPVEGHTKLYSMGKAFFTFFL
                     EYPHYFNIIMTYEARNCIYYPAQVSNGIGFYKNECQKIADNNTDLILKAIEYGIQEGS
                     IHTTLTPRQLMVILWGQVFGITQIIMMRQTYFKEAFGIDHTELFERFLIHTENSLKNP
                     I"
     gene            45426..46580
                     /locus_tag="PLV81_01970"
     CDS             45426..46580
                     /locus_tag="PLV81_01970"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HOM08901.1"
                     /translation="MDSLHSVFLISFFILTPAIIIYCCYRFPLINKIGPVLLCYIAGI
                     TIGNIGVLPDNAKQIQDIGSNISIVLGIPLLLFTINVKTWFKLAGKALLSFTLGSVAV
                     VIIVFIGYISFKHSIPDAWKIAGMLIGVYTGGTPNLAAIKEALQVDSTTFVIVHTYDT
                     VFSMLYIIFLVSIAQRIFLKFLPAFSVVNDEEKNAKTDEDIKLYGDIFTRKIFPQLII
                     ALLLSTVVVAISFGISLVVPQQYQTMIVILALTTVAILFSLSPYVHNLKRTFQFGMYL
                     IYIFCTIVGSMVNVDNLIHINMYLLFYVFIAIFGSLLLHGIFCKIFSIDTDTYIITSV
                     AGICSPPFVPVVADALHNKYIILSGITTGVIGYAIGNYLGITLGYLLSSM"
     gene            46638..48071
                     /locus_tag="PLV81_01975"
     CDS             46638..48071
                     /locus_tag="PLV81_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOM08902.1"
                     /translation="MNFDQLPHKGLSYTKVLSLMKQYGVNDASWKKGKTFSLVYHRDD
                     THTKFLQKAFELYFDENGLNPMAFKSLKQFEHEVVKMTAHMLHAGNEACGTMTSGGTE
                     SCLLAVKTYRDMARTQRPWIKKPEMVVPESIHVAFEKAAEYFGVKIVHAPLDKDFKVD
                     VKAVEKLINRNTILIVGSAPCYPYGVIDPIDLLSQIAIKKKLPLHVDACLGGFLLPFV
                     EKLGYKLPPFDFRVEGVTSMSADVHKYGFSAKGASVIVYRNINYLRHQFFIYEDWCGG
                     VFASPALLGTRPGGTIAAAWAAMVAQGEQGYMHNANIIMETTQKIQKGISSIKGMEIL
                     GTPHMSVFAYRSVDPEVNTFAIGDILEKKGWHVDRLQRPDALHAMITPVHAKVAHQFI
                     NDVKNAFEHVKKNPHLAYEGSAAMYGMIAHIPMRKMVRNNILKMMEGMYGPTGQIPDL
                     SMEEDTKEDFAMKAGKWYLKLRDKLIK"
     gene            complement(48085..49521)
                     /gene="rsmG"
                     /locus_tag="PLV81_01980"
     CDS             complement(48085..49521)
                     /gene="rsmG"
                     /locus_tag="PLV81_01980"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOM08903.1"
                     /translation="MYEQKISNYLKKSSLQFSNEAVQQLSLYLKLLLQYNDSHDLTRI
                     KDIDEIIVKHFVDSLYITSFIDVPSPLLDIGTGAGFPGIPLAIVHRNKSFILAESRHK
                     RIEFLSIVKNELHLDNVEIYPHLVTEKSFFNVHGVITRAVESIAETLHRCRHFLIKDN
                     LIIFMKGPAVDEEKIDEHGSYSLVLDKHYTLPDTTYQRRLVVYKKTTNEIKKIYYIAK
                     DGSSTDGIAVTSSENKRFKEFKKIALNPKKYNTTFVSGKKIIKELINSKPELCKYLII
                     YDEYVESDMEFLHLISNFGKERIIILKKSLFNEIDITEADQPVMAVQVPALTQWDYTC
                     QELSLMLPFQDPVNMGAAIRSAVAFGVKHIIVCENAAYPFHQKAVRASAGAVFHCTIE
                     KGPALNMLNTRTISCPILALDMEGEHIYDYKFPPKAILVAGTEGPGLPVIDKAVKLKI
                     PIHTVESLNATVAVSIALYEWHKQKKCI"
     gene            complement(49532..50473)
                     /locus_tag="PLV81_01985"
     CDS             complement(49532..50473)
                     /locus_tag="PLV81_01985"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOM08904.1"
                     /translation="MNKNKIIFIILTVSIISFLSISLYIVKIFNTQISDTTILKPADI
                     FANENEYITYIQSLNIPGVTFTVCQIEKGDNFWKIAKEYNIDIDTLIGANPLWESIVA
                     KIDQKVVIPSQKGVLHFVNNISEISELHDLYNVPENNVLTPSLLLYRIKSLVSKMDKP
                     VAVYIKDARPITDVMTPQLAKQFALREMFRSPLGGRLTSFFGGRVHPIFRQWGFHNGL
                     DIAAPYGTPVGAARGGVVISSGWMGGYGKAVIIQHDEGFKTLYGHLSTINVRPGQKVK
                     AGQFIGRVGSTGWSTGPHLHFTLWQYNKLINPMKVLW"
     gene            complement(50690..50762)
                     /locus_tag="PLV81_01990"
     tRNA            complement(50690..50762)
                     /locus_tag="PLV81_01990"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50727..50729),aa:Thr,seq:cgt)
     gene            50996..53656
                     /locus_tag="PLV81_01995"
     CDS             50996..53656
                     /locus_tag="PLV81_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOM08905.1"
                     /translation="MGSIILFNSFSLGTLIPFIFFMTTGLFLLNTKNKSRATEVMGKA
                     YVLMGLFNVGYLISSSIYHPYAAFHRWWTVFFILIAITYFGYFYFFFPTTKAPRAAKV
                     YLITGTIITLISLSAFIVVSLQSKIVYQFRGHYYDFDAEDISKYIGIIIMFGILCSYI
                     VAGWKIAISSKAERKVILLLIFFSMVGTIVPSITNTLSRAGTIDREVFHTTWTLFNLL
                     GFFLNFIAYINNSKDRISFLGKLIGIILVTVLCLLQIVGFIVLQDKENNFDKIYYQAT
                     LLAVSNESPQAEHLYISKYNVNDNKWLKKLPENLDEKEITNELINTYIWNQIFTASED
                     NLSQSIISIITSNTNKFFRGYALSLQNYCTHNANVLNKQTLVDYCKMLDKKVYHLNHQ
                     ISSLNTKNFRSYLKQILSKPDKAITGFVTAINEFIDQSPEEGEQLKQAVKQFLLPVQF
                     PQQRTYHTTLDNKSHIIAYRIIEDDTVTEVGFPYELYRKHMNSTVITLIIIVISAYIF
                     IRFAIRLFFAGILIKPIRILTEGLREVDKGNFNIHLPVQSGDEFGFITGEFNKMVESL
                     RNLFQSTQSKSEEVKRLSSDLNVSATKLYDIARELSTIVEETSSAYEEMSSSFESNLS
                     AIKQQADSMDVIKNDIEGIDVSSNQISQRITRLSHSIEQAVKQSEDGEVTITKSINAI
                     ESIAEYLKEVEQTIVNINEIADKINLLALNAAIEAARAGESGKGFSVVADEVNKLADQ
                     TADIVKGIHSTISEQARMITNEIQYISKATEAIKAIRKGITETFNVLKDTVDFTNELN
                     IKNKDIKTKLESFKELSGSVYNFSMEQKVTLDELTKAVNAIIDISQKALESAEFVQGF
                     SRILDLSAQELSESIGYATNGLKQSENNDD"
     gene            53931..54200
                     /locus_tag="PLV81_02000"
     CDS             53931..54200
                     /locus_tag="PLV81_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOM08906.1"
                     /translation="MPTYEYVCTSCNDKFEMFQSIKDEPVKTCSKCGGEVRRLIGSGA
                     GIIFKGSGFYVTDYKNNGNGKTSKSTSTEPACATCESGSCAADLD"
     gene            complement(54263..56095)
                     /locus_tag="PLV81_02005"
     CDS             complement(54263..56095)
                     /locus_tag="PLV81_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08907.1"
                     /translation="MSIDNKAQRFPFIQGLLAKESYVYDILAEIVRMQELSLPDGKRE
                     MATMQFLQSSRNIPIPFTTSRNNQINYLITQLLNSYPDHVKKLITFSFSVDEKTKKLD
                     INEKEEYCVDIQDEEFNRLQSIRDEISKQNLLTLLKQYRLFNLSKLIKKSINKNDLNR
                     IIKQELGNSIFTFKHFTIDKNHIELAEDYCFHADNYQAVKSTLNFKTLKNIIAQHADM
                     ILRRLKKFGIISYDFSDYRDAKLDYIFNVLIDDLASLLSESDLAEVKNLQAFRNCLLK
                     VDSLLDPVDAFHNDIVTVIREHKICLQDDIISTIPEINDSLIGKWRESNYRKTAKIIV
                     YNDSETDQWYYVDGPVFINYLSDLNQLMYYQKEKFEKLGYLEKEKLKRNYNILIKIAE
                     TFITDNIDDLTLNQEQKEILQKILDDYKNQKKEAEISTQIPVNKEIKQKKSLISRIIE
                     AILSLFRRKKIIKPITQAQVFSSPVHQLSKQARNLYDTISSINRNIIALSEVMDITDE
                     NETQVSEIIEELRDTGLKIVIPIYNARRVLYPHRSQKLLIPDIEYILVSPVVIRSYDA
                     IKEFCDSLVGKKINGEIIPSSGIIAIEKYLHTLYRQKRNLMMKK"
     gene            complement(56185..57435)
                     /gene="hisS"
                     /locus_tag="PLV81_02010"
     CDS             complement(56185..57435)
                     /gene="hisS"
                     /locus_tag="PLV81_02010"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOM08908.1"
                     /translation="MIQKPPGIEDIFPDRTPKFNHIVNTAKEVFRLYNYREVILPVME
                     YTEVFARGIGDGTDIVTKEMFTFEDRGGRSLTLRPEGTASMVRAYVENGEYNRLALCK
                     FFYYGPMFRAERPQKGRLRQFNQFGAEVFGSDNPWYDFEVISLMNTIAQRLGITEYNL
                     LINSIGCPDCRKPYIGELFTYYSTHEHELCEDCKKRLRTNPLRLLDCKVEGCIALKKN
                     APKITQFLCQSCKEHFSQTSNFLEKHSIHYIHDPLLVRGLDYYTKTTFEFVTTKLGGQ
                     NAFAAGGRYNNLVELFGGKPTPAVGFAAGIERIELLLEQLSVKEHLDVYIAYSGDNAL
                     PVVIQTAEELRKNNISCDFDPAAKGFKTQLKRADRENARFSLIFGEDELANKCATIKD
                     MSTGDQMTVEQDSIISFLQGKLTQ"
     gene            complement(57468..58682)
                     /locus_tag="PLV81_02015"
     CDS             complement(57468..58682)
                     /locus_tag="PLV81_02015"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOM08909.1"
                     /translation="MALKTGKNIELVAPAGNFEKLQAAVHYGAHAVYFGGQQYNLRAT
                     TKNFSIHEIEQAVAYAHTHNVKAIFLLNAFLHESDIPVVTEYIQHIKHIPFDAIMVSD
                     PGMSLLVKEHLNVPLHLSTQMSTLNHLAVKFWASHGISRIVLARETTLEEIKQIREHT
                     DIELEIFCHGALCISYSGRCLLSRYLSGRDANQGNCSHPCRWKYSLVEEKRPGNHLDI
                     IEYSCGTEILSSKDLCLINKLPDYINAGVNAFKIEGRMKSLYYTANVTRIYRHAIDTF
                     LNGGDSNRYSDFWHNELDLVSHRPYTEDLFNEFNNQTFDGVPYIKKTLFMGYCIDQQK
                     NLVKVFNPIYKGDSYDAIYPVVNNQINDSPVTVIDILVDGNSVSMAQPNTIATIIFDK
                     PLGQWAILRKRL"
     gene            complement(58654..59664)
                     /gene="mltG"
                     /locus_tag="PLV81_02020"
     CDS             complement(58654..59664)
                     /gene="mltG"
                     /locus_tag="PLV81_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOM08910.1"
                     /translation="MKEIKAAALSFIILFIIFAIVFWFFNSPPYTMKGDTITVRPGMN
                     IHQVAYFLKSKNIIKSASFFYILGRVLNATDVKAGNYRIYDGESSFSILIKLTRGKFV
                     TKRITIPEGYTIYDIARVLAANDICTEGDFLYWAQSPSFLASLGIRAPSAEGYLFPDT
                     YVIPENTDPRDIIIRLVNQTKKVIHDIQESIEVQYSNLHQILTVASLVEKEAKINSER
                     ILIAAVFYNRLKLKMKLDCDSTIQYGLKKFGKRLTYDDLDSDTPYNTYKYTGLPPTPI
                     CNPGKRSILAALNPAQVPYLFFVSRNDGSHYFSKTLSEHNRAVDFYQKGIRNGFKDRQ
                     KY"
     gene            59772..61766
                     /locus_tag="PLV81_02025"
     CDS             59772..61766
                     /locus_tag="PLV81_02025"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM08911.1"
                     /translation="MCRKNIAILLFLFCCVVLYGQEPVKLQQKNAIYYNKKGWERLET
                     GNAYSAIVAFQSALAQNPKYKEALLGLGYAYLHTEGYTESVKIFDAVLKLYPNNEQAL
                     IGMAKAYTGLGRYREAMKIYDTLNELSYTNNDSRYGMAYLYYKMGNMLWAKRMLNRIL
                     QNDPYYYDALLLLADIKAKDKRFDDAIDLVKKAIDAKETYPDAFVKLAELLYLKYYYS
                     GDKAYLQQAVDELQKALIINKTHLQANRDMGYLLYIQGDYQNAISYYEAIITHYPHIA
                     SNEILYNLGLMYEYKGDSNQSLTYYQKALSKDNLNSLIAMKMRQLCLLEHIKIGHPVR
                     IQSSNDDFSSSQELRKKGFPDLSMLFVRLSLALNPNNTTARQELMRIYETLGYFEFYY
                     DEIKKMHTLNPTQKTQDMMSIAVMKRRDKLFFRLGYDKEELPRDVPLVLVLDFTPTVA
                     VPAFADAGSVIANSINFAMGQFGRYRNFPLSQRQAITKEFASNNLDDILSILNDKINS
                     NELPQISYMLYGEYSLSGRFIEINAKLMDVKTGVVIAHYNESDNTKYSLQTVSLDIAK
                     NLYSTIPYWGKIQFYSDDMVSVNLGSIDGIEIDSMLETFVDNEDTIDSEKPRKKVILQ
                     VKEVDTRVLLAKPINPKDIDLLSKGQEVYPVSKRRAKKIN"
     gene            61896..62684
                     /locus_tag="PLV81_02030"
     CDS             61896..62684
                     /locus_tag="PLV81_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOM08912.1"
                     /translation="MKIAVLVKQVPDMESKFKIIGDSKIDESQIAFKMNDFDEYAVEA
                     ALQLKEKFGGEVIIISAGPERASKEIRQAFAMGGDWGIQIQDEQVDAGDNFVVASALC
                     KAVESLGGVDLVLTGVQAEDDQAAVTGVMIADMLGLPHCTNVVKIEVGGDGKSMTVNR
                     ELEGGLNEVLEMAMPAVLTIQSGINQPRYPTLPGIMKAKKKRLDVKKAADLGVSAVGD
                     AGSKTKFVKMFFPVSDHKAEIIEGDPKTAAAKLVEKLRNEAKVI"
     gene            62696..63646
                     /locus_tag="PLV81_02035"
     CDS             62696..63646
                     /locus_tag="PLV81_02035"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOM08913.1"
                     /translation="MARILVIAEQRNGKISDATLELCKAAKALASAMGASPAAAVFYK
                     DDSLAKEVAQYIPEVFAVVDPKLEVYSADTYVAAAKAVVEAQDVKGVLVPHTYDGTDY
                     AAKLALNLGCGIVTNCNKFEVQGGAVVFTRNTYNGKIQEQRSVKTDKFVATFEKGAFE
                     KEAAGGAGSVTAVSASIPEPRRKFKQFVETMAGSVDISQAKIIVSGGRGTKEKDKYND
                     IIVNFAKKLGGEYAASRPVVDAGWTDAARQVGQSGKTVTPDLYIACGISGAIQHVAGM
                     KGSKVIVAINKDPEAPIFNIATYGIVGDLFEVIPAIMEQL"
     gene            complement(63906..64676)
                     /locus_tag="PLV81_02040"
     CDS             complement(63906..64676)
                     /locus_tag="PLV81_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08914.1"
                     /translation="MFKRFFIIVAIMAVSIFLIQPGYACFDTFLFLHHKSMVYPTGFF
                     AADVLGEYSFNDTDAPDQDSYFTNYSMYYGLAERVSVQFGLTQAETTRAENKYSLESW
                     SVRGVFNTIRSNSGNYFMDLVAEHHTGVDIDENMAHFSIPNLLYLSNFIVVIHPVYEL
                     TYSKNDREHTFGGHGGLFYNINNIGIIGVGGEFASAQSSSALGKRFTEGEAAASLFIG
                     FNLGNVYFQNEFAKGLNNSRDFGFAATLKFFFNTGMSI"
     gene            complement(64778..65521)
                     /gene="recO"
                     /locus_tag="PLV81_02045"
     CDS             complement(64778..65521)
                     /gene="recO"
                     /locus_tag="PLV81_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOM08915.1"
                     /translation="MEIIKSEGIILKKIPVLEADVADIIYTQNHGKRTFIFKGIKKTK
                     RRSQAAIEPGSVVHLVYYYNEQKAMYHVKEFSLLLDTVSIRDNYQAMITLFLLLEVVE
                     KTTGHNDPNITIYKLLKGAIETLHKTSAYTFLSLSFILHYLKLSGIIPDYLHCSVCHK
                     EITDDLYLTEYQLISYCNQCAPNNAFVFKATAPLIYEILTKKFLNIETDIYNSIDIQK
                     LLFHTLLFIDHYFNIHIKTKELLYNNTKL"
     gene            complement(65528..66370)
                     /locus_tag="PLV81_02050"
     CDS             complement(65528..66370)
                     /locus_tag="PLV81_02050"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOM08916.1"
                     /translation="MASDEQKPKKTNNNDAVSLLKQKFFKFIHKKNISVPVDKLAIQK
                     QEMIQGILSLSTKNVREVMIPRVDINAVEDSIQLKELVKRVIDEGHSRIPVYSGTIDN
                     ITGILYVKDLMPFIIDRRMKFNIKKLLHKPYFVPETMTLDELLLQFQKQNLHLAIVVD
                     EYGGVAGIVTLEDILEEIVGEIRDEFDEDEIPEIQTISKNTWEVDSRLSISDFNEHTG
                     CAIPDQEFDTIGGFVFDLFGKIPKKDEQIKYNNVTFKIKDIQGTRIGRIIVTVTPQKN
                     SDTY"
     gene            66607..68964
                     /locus_tag="PLV81_02055"
     CDS             66607..68964
                     /locus_tag="PLV81_02055"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOM08917.1"
                     /translation="MDSMLFWIAPVGSIIALLFAFIFYKMVMKADKGNETMVEIAQAV
                     REGAFAYLKQQYKVVSLVFVILVILLSILAYYGIQNPFVPFAFLTGGFFSGLCGFLGM
                     NTATNASSRTAQGARESLNRGLQVAFRSGAVMGLVVVGFGLLDISLWYIILNWIYDNN
                     IMGFSQELAEKIGLLTAGQSFDVSLITNEAFKHGKLVEITTTMITFGLGASTQALFAR
                     VGGGIYTKAADVGADLVGKVEAEIPEDDPRNPATIADNVGDNVGDVAGMGADLYESYC
                     GSILSTAALGAALPLVGIENVNAVIAPMIVAGLGILFSVIGIFFVRTEEDASMKTLLK
                     ALNRGVWGSSILLIIALAALAYVNIISWGVFGASIAGLIAGLVIGKSTEYYTADDYSP
                     TQGIAKQHTMGPATGILDGISVGMMSTAIPVVTIVLGILAAFGLAGGFSSDPNAVSKG
                     LYGIAFAAVGMLSTLGITLATDAYGPIADNAGGNAEMSHLGKEVRTRTDALDMLGNTT
                     AAIGKGFAIGSAALTAMALIAAYIEEVKMWVSKYTARSAEGFFQVGSVKFYNILPENI
                     TADLNNVVIKHATIGDFVRAYDITLLNPMVIAGLFLGAMMAFLIAALTIKAVGKAAGA
                     MVEEVRRQFKEFPGILKGTQKPDYARCVMISTAGAQRKMILPSMLALIVPIAAGLLLG
                     VAGTTGVLAGALAAGFSLACMLNNAGGAWDNAKKYIEKGNYGGKGSDPHKAGVIGDTV
                     GDPFKDTSGPSLNILLKLMSMVSVVFAGVVVHFYPQIAKFLGMMK"
     gene            complement(68999..69289)
                     /locus_tag="PLV81_02060"
     CDS             complement(68999..69289)
                     /locus_tag="PLV81_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08918.1"
                     /translation="MKAGLLITGNGALIYLTSYDSFMNDKLKEKLASKGITKFIAYEI
                     PVEEAKKRYGGHFDIVIRDLHETDDLRILDYDGSRAFKLFSFKELGKPVYHE"
     gene            69835..72822
                     /locus_tag="PLV81_02065"
     CDS             69835..72822
                     /locus_tag="PLV81_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOM08919.1"
                     /translation="MELARYRIEIYYTIPDSRGTVLQQKLQRLGFPVSKCIRTDNYLI
                     NIPLNDSQIEKVAQALVQPVTQNYTINKPYAPSDFRYAVEIGYLPGVTDNVAHTTREI
                     IEDIFKIQCDPEKCVFTSSTYFFWGGLTIDAIHEISLELYNPLIQRSLILSYDEYIKN
                     GGMGNAIPVVTLHEDTKAEVVSLDVPEDELIALGKEGIKNPDGSRRGPLALDMLSLEA
                     IKKYYRDIEKRNPTDVELESIAQTWSEHCKHTIFAAQLDDINDGIFKHYIKEATLRIR
                     REKGKHDICVSVFSDNAGGIEFDDDYVIADKVETHNSPSALDPFGGAITGIVGVNRDA
                     IGFGLGSKPVANRYGFCFANPFDTKPLYKSKDPNSKMLSPRRIMEGVIHGVNVGGNCS
                     GIPTPQGFVYFDDRYKGKPLVFVGTVGLIPKKIKGKSSVHKKAMPGDNIVMVGGRVGR
                     DGIHGATFSSEALTSGSPATAVQIGDPITQKKFSDAIVKEARDLGLYHSITDNGAGGL
                     SCSVSEMAREAGGFIVYLEKVPLKYPGLAPWQIWVSESQERMTLSVPDETLEDFLNLM
                     KRRGVEAWVIGKFTDSGRGIVYYNGEIVFNIDLHFLHEGLPQKTLTSTYTPKSHPYPE
                     FDDPENITEHFIAMVKRLNTASFEFISTQYDHEVQGNSVIKPLQGKGKVNGNATVIRP
                     LLESTKGVVLSQGLYPSYSDIDTYWMAACSIDTAIRNAVATGCDLDKLALLDNFCWCD
                     STNPYRLGQLKEAARACYDFAVAYKTPFISGKDSMFNDFKGYDEHFNEVFISVPPTLL
                     ISSIGVVSDISYCQTLDFKNAGDYVYILGITRDELGGSEYLAYMGETLSGERYIGNTI
                     PHVDTIMNTKVYRVIEKALRAGLIASSISIERGGIALAIAKSAMGGLLGANIDLRVIP
                     HENINRNDTLLFSESQGRILVSIAPENKTQFEKLCKDIPFAKIGQVTDNSLIRINALN
                     GNEIVSASVIDLHSAYKSTFIEF"
     gene            72831..73640
                     /gene="purQ"
                     /locus_tag="PLV81_02070"
     CDS             72831..73640
                     /gene="purQ"
                     /locus_tag="PLV81_02070"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011170122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HOM08920.1"
                     /translation="MAKPKALILTGYGINCDEETRFSFEIAGAHATIIHINDIIENKS
                     LLNSYQIFAFPGGFSYGDDTGSGKALANKIKYNLYEEMMSFINRDTLMIGICNGFQVM
                     VNLGMVPALTQPIGTVEVSLEHNATFRFQCRWVDLAINSHSNCVFTKNMERLHIPVAH
                     GEGNFVAPQHIVDILSKNNQIIMQYIKPDGSFAQGQFPYNPNGSIMDIAAICDTTGRI
                     MGMMPHPERHIYFMQRDDWTFLREVSKRQKKEFPQYGEGLQIFKNAVEYYK"
     gene            73962..78020
                     /locus_tag="PLV81_02075"
     CDS             73962..78020
                     /locus_tag="PLV81_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOM08921.1"
                     /translation="MQYSEIKSIAYNRLLAITENKKEDISSWLKDQTDDADLLIQSRQ
                     FRNLLQKYFTYNNAESYNTLYEFFDFYAKNNDISEVLLVNKDGTILFSLYKHIKLLSQ
                     DTLQYLQYAANTNKPVFTDIHIDNDDFNYHISLIVPVNPDFTARSNHFIILISSAENF
                     IFPVLQRWTGLTQTGETILIVKDRPTLYIFKSYLAQKVPEIIKVNTESLSHDNVIRMA
                     INGVKGYKYGKDLKNIHVGTYITSIPQTPWIILTKSSEAELFQSWYIIVTISIALFIL
                     SIILLFSIITIMQKEQQRTLYQELSQKQDEQLKIYQIYKYIIQSIGNGLIITDNSGNI
                     EFINTIGELLTGWHNDEAEGKHVLDVLHILSQNNTITSKDDLFQIIAEGKFRGEYHNS
                     VLISKTGLEIPVNYHGTILKSGHNQNLGAVIVIEDQTRESFLNLINDIRLKFINFSIN
                     HTKDDFIKNVLHEILTITNSQIGCYISYKYLYKQITGLKELPNSYHTYCIDQNIDCSV
                     DYEKVWKECQKNKGHIINNDHTQYFYCEELKNGTDHPRELFVPVFRENAIVGIIGVAN
                     KHKDYTYADVSKLSILSDMVYEILSHKENEEAYKNLVMLSNELACIADLKTTRFLLVN
                     PSFSKTLGYSEEELTSKSFLDFIHPDDKEKTIQIINEKLLKGEDVITFENRYKCRDGS
                     YVWLDWNSHPVISQGITFATAHNITERKIAEEVLKENEKKFRELFVHSNDGICLHELV
                     YDNNGNPIDYKIIDVNPQYEDILSLNKEDVIGKLATEIYNTSEPPYFEIYKEVAVTGK
                     STTFETYYEPMNKFFIISVFSPQKGQFATVFQDITEIKLAAKKIEESEEKFSLFMKNL
                     PLVVVIRDINGRYIYLNDEWERVMELKKEDWLGKTPYDVFPKEDAEKLLQGDREAIEK
                     GVTEPSEFTLKHKSGIKWWMANRFVLYDENKKPAYVATIYIDITERKKAEEEREKLKD
                     QLIQAQKLESVGRLAGGVAHDYNNMLEVILGNAQLALMQIDSKEDVKNYLEEITKAAK
                     RSADITKQLLTFARKQISEPQIVNINTIIESMLKMLRRLIGEDIDLQWILSENLWNVY
                     IDPVQVNQIIANLCVNARDAIQNNGIITIKTKNVTLDEEYCKDIPDCLPGEYVMLSVS
                     DNGIGIDKEIIGKIFEPFFTTKEVGKGTGLGLATVYGIVKQHHGFINVYSEKGKGSTF
                     NIYLPRHRGLKEAENSVTGNSWVYGNGETILVVEDEPSLLKMVQVMLERLNYTVLSAQ
                     SPPEAINIIKSKGQSIQLMITDFIMPIMDGLQLAQEVAKLSPHIKFLFMSGYTDRILF
                     ESNSSNKETNFLHKPFSISELAKKIHDILENK"
     gene            complement(78087..79403)
                     /locus_tag="PLV81_02080"
     CDS             complement(78087..79403)
                     /locus_tag="PLV81_02080"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOM08922.1"
                     /translation="MKFKRVFALLAIVLLFTACSSTDKKGQTNAASTKNGPGKIYWDD
                     KSLKGEGNFTNGVKTGKWILYHKGTGEKLAEGEYVDDKQQGFWTYYHKNGAKSLEGNF
                     DQDQKTGEWIAYYPEGPVMWKATYVVKEKTESGFTMKVGAIEGIKTWYYQSGRIWKEE
                     QFTNGEQTGRSQEYYENGNPKIIAWYKAGQLDGQCNKWYENGKKLEEGRYKENKKNGN
                     FKFYFDNGQISATGSFIMDKPEGDWKFNSKEGLLQKEGKYKDGKEIGLWNFYMYPSRV
                     QRIKIMELPLQGGMIDNTNENAKLYDEKGQLIGYGQLTGIPKGIYTVLKDGKPTGEMT
                     SSSVPNDDPKEKITYTWTGKWETPKKNGKWTEYYTGTKSVKLEGTYLMDKLNGPYKEF
                     YPNGKIKSEGEYLNGKMNGEWKFYKADGTLDEAQSGKYMLGKKMNF"
     gene            79588..80382
                     /gene="flgG"
                     /locus_tag="PLV81_02085"
     CDS             79588..80382
                     /gene="flgG"
                     /locus_tag="PLV81_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004494936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HOM08923.1"
                     /translation="MMRSLWTAASGMIGQQFNIDTISNNLSNVNTTGFKKLRAEFEDL
                     IYQTVLMAGTPATEISEVPTGIQVGHGVKVAATQKLFDQGSLQATGNKLDIALEGEGF
                     FKVQLYDGTIAYTRDGSFKVDSNRQIVTSNGYLLEPPIVLPENFIMETLSISQDGRIT
                     VKVVGDDEPIEVGQLEIARFVNPAGLQNIGGNLYKTTPASGEEIAGPPGIDGMARTHQ
                     GFLEMSNVKVVEEMVNMIVAQRAYEINSKAIQTSDSMLGTAIGLKR"
     gene            80382..80909
                     /locus_tag="PLV81_02090"
     CDS             80382..80909
                     /locus_tag="PLV81_02090"
                     /inference="COORDINATES: protein motif:HMM:NF024544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella basal body P-ring formation protein
                     FlgA"
                     /protein_id="HOM08924.1"
                     /translation="MNRIIVATIIISALFQSLLYADLAVYLKSNTAPKNNLSISDIAQ
                     IDGDESELVKKITIPESVLDDGYITKNEVYKLLKDVVTGLLVIHGSAVKLVVPESIST
                     SDSSLQELSPIYNELVIVKGDLVTVKLIKKSIVIELIGKALQSGKTGDEIKISLQSGK
                     TVTGKIVNNGVECYL"
     gene            80906..81532
                     /locus_tag="PLV81_02095"
     CDS             80906..81532
                     /locus_tag="PLV81_02095"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HOM08925.1"
                     /translation="MKKFLLAIALIMISAVYADTIWKDKNIYTAQDTIKVGDIFTVAI
                     TDISQMRFTLTLANNDAFTVTSTPDGNITPFLPQVNANKKATKNNSTNLQHRGNITLH
                     IAATVQQRLENGNFRIAGTKTFSFSGVTIVFNVSGIVNPASIKGGTINSSDIANLVLQ
                     IQGVKEGVRITREPLKEGETASPALTEQEKQQIIIQYLQRILGELTQQ"
     gene            81529..82521
                     /locus_tag="PLV81_02100"
     CDS             81529..82521
                     /locus_tag="PLV81_02100"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOM08926.1"
                     /translation="MMNTVYKIIICIMTVTFFTVNLCAEVPVKIKDLVAIDGLKENQV
                     YGYGLVIGLQGTGDSKVKLTQSSLSNVLKNLGLNDENINSKNIAAVLVTATLPPYVRI
                     GDRIDVTVSSIGDAKSLENGVLLQSALKGADDTTYVVAQGVVLIAGDKRKGATIGRIV
                     SGGIVEKEILPDIVKDGNIKFSLLNFDFSVAKEIKTAIEKKYPESKPIIDKGYIQCTL
                     PQNVTVTDFIADIENIEIVPVYSARVVINERSGTIVMGGDIRVSQAVISKQGVTITIE
                     ETRKKGSMYLLKDAATVKDLVDSLNYIGMSTQDIIVILKAMKQAGCLHAELIVQ"
     gene            82529..82906
                     /locus_tag="PLV81_02105"
     CDS             82529..82906
                     /locus_tag="PLV81_02105"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HOM08927.1"
                     /translation="MDTTIKNIIDSQKTVQSINKQKDIEQKLHNTSTDFKSMLNNAIA
                     KNNDKPVDKKLMDVCIEMESLFVYQMLKEMRKTVHKENDLLYGGMAQEIFEDMLYNEY
                     ALQMSKTANFGLAKTLYEQLSQK"
     gene            83011..84315
                     /locus_tag="PLV81_02110"
     CDS             83011..84315
                     /locus_tag="PLV81_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOM08928.1"
                     /translation="MHLFELFIGWRYLKAKKSQGFISFNTFLSIFIVAIGVFVLIVVM
                     SVMTGFQSQIRDKILDVDAHITISRYYADTDSEGIANYRELSDKIKNVKQIKTIMPFV
                     QGQGLVRVKSYIFPVAIRGYGNPGEVPKDITKFITAGDKDFKNATSIAIGEEMAFNYN
                     LRLGDYIEIIVPKGRLTAREGITPGIGRYRIASFFKTGYYDFDTKLILLTIPQTQRLF
                     GIGNIVSGLALKLFDVYDMDRTASLLQSIIGYDYQVITAEQQNQNLFYALRLEKLIMT
                     IILFLVIISASFTIMGTLVMVVMEKRKAIGILKAMGAQPYSIMIIFILEGFFIGLIGA
                     IIGVITGLATAINLEAIIHWVERVINSIMSWIYITFDLGVFYPVSLVPKNVYYIDSLP
                     TQIQPEFVMLVAILAIFIATVAAILPSWHASRLHPVESIRYE"
     gene            84363..85040
                     /locus_tag="PLV81_02115"
     CDS             84363..85040
                     /locus_tag="PLV81_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009237786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOM08929.1"
                     /translation="MIKTENLFKQYGYGTNSITVLNNISISINKGEIVSVMGPSGAGK
                     STLLHIIGCLDTFQGGRVQILQQDISPFSYDELSGFRNQHIGFIFQMHNLMPEFTALE
                     NTMMPLLIKRHAYSKAKQKAFELLEHFGVADRANHKPAELSGGECQRIAVARALITDP
                     DIILADEPTGNLDRKNSDILIELLLKSCVEKGSTVVIVTHDQYIASKTQRVITLIDGM
                     IQKDMSN"
     gene            85057..86658
                     /locus_tag="PLV81_02120"
     CDS             85057..86658
                     /locus_tag="PLV81_02120"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF027971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOM08930.1"
                     /translation="MKIEFVGGARTVTGSSFIVKNNNFTVMVDCGMFQGTRELRERNY
                     LNLIYAPQQIDCLLLTHAHIDHSGLIPKLVKEGFAKPIYATKATCELATIMLPDSAHI
                     QEQDAEIINKKRKKIGREFIQPIYTSEDAERCLQYFNPVHYHETVSVHPQITARFYDA
                     GHILGSSFIEMHVQENNKEQVIIFSGDIGQKNQAIIRDPELPEYADILLIESTYGDRL
                     HKSKEDTYNELKHVILDSYNKKGNIIIPAFAIERTQEILYTIAQLVKKGEIPPIPVYL
                     DSPLATSATEIFMNNQDCFDEAMLKLLKTGDSPLDFPNLHFVQTVEESKWLNTNAKGA
                     IIIAASGMCTAGRVKHHLLNNLYKPESSVVFVGYQAEGTLGRRIIDGAKLVKIYGENV
                     AVRAKIYTIGGFSAHADKEGLLEWMSHIKNPNLKVFVIHGEEKASTAFADEIRNRFGY
                     SVYVPHWGEIIDFDTMKSEYAQYSPIEKTDNIESEIERISLLLSTIKMRYQKAKEEGR
                     RINWAQVENDISDVRELVTMINDEL"
     gene            complement(86715..>87890)
                     /locus_tag="PLV81_02125"
     CDS             complement(86715..>87890)
                     /locus_tag="PLV81_02125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOM08931.1"
                     /translation="PREMILDMDAEKIHFPKEFLKEAGKRNLLGIRLPKKYGGRGLKW
                     VDDCIVAEEIGVASYSLACLWGVGADIVCEAIVEFGNEELKQEVVVPLLKGDVYAAEC
                     LTEPRGGSDFFGATTIAKKDGSDWIITGQKRFIVGAEGADWFMVYAVTNPEGKPHERL
                     TCFMVPRTKGVETKYIYGLMGVRGGGAGRLILNEVRVPERYALNGINGGFDVFVKNMI
                     PERLGTAAMTIGSVRPAIEIATRYTSMRKAFGQPIANFEAVGFKVADSVMLLDAARSL
                     VYSTALAIDSGKVHPGRIRRMVSESKKFVTESAWTIANNCMQVMGGIGYTNVYPIERI
                     VRDIRLSMIWVGTNEIMDLIIQNEWYKEYFKVISKENIRDVEQDAPGADQVEEKVYE"
BASE COUNT        28773 a        14735 c        15233 g        28949 t
ORIGIN      
        1 ctaattaaac gaaaggaatt tggcgcacat cctggtgata tctcacattt tcaggcttta
       61 atgacagcac tggctgctac tgttggtact ggaaatattg ctggtgttgc atcagccatt
      121 gctataggtg ggccaggtgc aatgttctgg atgtggctta cagggttatt tggaatggcc
      181 acaaagtata gtgaagcagt tcttgccgtc aaatatcgtg aagttgatgc actgggaacc
      241 atgtcaggtg gcccaatgta ttatatagaa aaaggattaa agaataactg gcttgccatg
      301 atatttgctg tatttgctgc atgtgctgca tttggtacag gcaacatgat acaatcaaac
      361 tccgttgctg aagctatgaa agcaaccttt aacatcgatc actggattac cggtattgta
      421 ctttttattt taacgtttct tgttattatg ggtggcatca aaagtattgc ccgggcagct
      481 tcggcgatag ttccaataat gattttattt tatataatag ccggaacaat aatattaata
      541 atttaccggg ttaatattct acatgctttt gaacttatat ttgtgcatgc gtttacgcct
      601 gtagctgctg ctggtggatt tgctggtgct actgttaaaa tggctatgca gatgggcgta
      661 tccagaggtt tattatctaa tgaatctggt ttgggtagct caccaatagc tgctgcagca
      721 gcaaagactg atgaaccggt aaggcaggct ctggtttcaa tgacgcagac ttttattgat
      781 accattgttg tgtgtacgtt tacgggtctg gttatactgg taactggtgc atgggaaagt
      841 ggaaaaacag ccgcagcatt gaccgaacat gcttttagtt tgggcttaac caggaacctg
      901 gggtggtatt gttgtttctc tcagccttat attttttgcg tattccacta ttttaggatg
      961 ggcatactac ggtgaaaaat cggttgaata cattgcaggt gaaaaggcaa taaagccata
     1021 tagagtatta tgggtgatag ttgtttttat aggagctatc ggaaaattag aaacagtatg
     1081 gacgttaact gatataatga atgggctcat ggcattccca aatttgatag cgttgcttgg
     1141 tctttcattt gtaataaaaa atgaaacaaa tcttttcttt aagaaacata attcaatata
     1201 atacattcgt taattattag tttcagagaa agttacagta agcaactcac tttacataac
     1261 aaggttgttg tagtgatatg atgaaatctt atataagact atgttatata atactaatta
     1321 ttatcctgtc ttcctgcaca cgctttgaat cagtgcgtga tccatatact ttatatattc
     1381 atatttcagc tgaaccaggg catcttaacc ccataacttc aaatgaagct atagcttctt
     1441 caattaattc atatatatat gaaacactgc ttgataggga ttatgataca ttagagttaa
     1501 aaccacaact ggctacaaga tgggaaattt catcggataa attgcattac cgcttttatc
     1561 ttaaagaaaa catttactgg agtgatggaa aaccttttac agccgatgat attgtatatt
     1621 cattcaagat aataaaagat ccacgtgttg ctaatgcacc gttaaaagtg tattatattg
     1681 atgtaaaaag tgtaaaaaaa atttctccgt atattgttga atttacgtat gctaagcctt
     1741 acttcctggc gcttgaaatc tgtggaagca ttcctatcgt tcccaaacat atatttgatg
     1801 atggaaccga tttcaatact cataaaaaca accgtttccc tattggcaca ggaccttata
     1861 aatttgtaac atggaataca ggaaaaacca ttgaactagt tgtgaacgat agatactggg
     1921 gtccaaagcc agatataaag cgcatagtat ataaaataat tcccgaacca aatgttgcac
     1981 tacagatgct aaaaaaaggc gagcttgatg tcatggcatt gaggccaata cagtgggtac
     2041 ggcagacaaa ttcaaagcat tttcaggaat ctttttataa atttatgtac tatcagccat
     2101 attataacta cattgggtgg aatgcacgca atcctttatt taaagataaa aatgttcgta
     2161 aagcgctgac catgttaatc aaccgtcagg caattcttga tagcttattg tttggattgg
     2221 gaacagtcgt gactggccca ttttatattc atgataaata ttataaccat gatatacagc
     2281 ctttgccata cgatcctgca aaagctaagg aaatattgca cagggcaggc tggagcgata
     2341 gtaatgggga tggcatcctt gataaaaatg gtgtagcctt caaatttaca ttgactatcg
     2401 cttcagcaag caagtttgct gaacggctgg caactatctt gaaagaagat ttttcaaagg
     2461 ttggcattca gatggacata aaccgttatg aatgggctat ttttgtaaat aaagtggata
     2521 agcgtgattt tgatgcagtt accctgggct ggtcattgtc atgggaaggc gacccctacc
     2581 agctatggca ttcatcacag gtacaggcag ggtcaaattt ttgttatttt attaatgccg
     2641 aagcagatga aattattgaa aaagcccgca aagagtttga tgtagaaaag cgcattaaga
     2701 tgtacaggcg tttccatgaa ataattcatg acgagcaacc ctatacattt ttatattgta
     2761 ctccagcact ggtggttgtc agcaaacggt ttgataatgt tattgtccat aaaagaggcc
     2821 tgaattatac tgaatggaag gtcaagcagt aaaaatgaaa tactatattt taaaacgaat
     2881 tcttattatc ataccaacat ttattggcat aacatttatt acatatctta tgatacggct
     2941 ggcacctggc gattatactt ctttacgagc aggtgtagaa ggtgaattga aagcaggagc
     3001 cctttcaaaa gagatatttg aacaggaaaa aaaactctat gggcttgata agccagtaat
     3061 tgtgggttat gcagaatggt tttataaaac agtaaaactt gattttggaa cttcacgtaa
     3121 agatgggcgt aaagttatat cccatattgc cgatgcattg ccaattacat tagcattgaa
     3181 cattctttct atcattatag tgtatattat ctctatccca ttgggcattt attcttcgat
     3241 aaaaaaagat acagtgcagg acagaattat aagcctcaca ctatttttgc tttattcttt
     3301 accttcattc tgggttgcgt tgttgttgct aaaatacctg agtggtgggg attatctgaa
     3361 tttattccca ttaggcggtt tatattcaga ctggtttgat cagttaaatc tttttcagaa
     3421 agcaatggat atactgtggc atttaattct gccagtagta acattaacct atggtggttt
     3481 tgcatttcta tcacgctaca cacgggcaac catgctggat gttattaatc agcaatatat
     3541 tattacagca cgtgcaaaag gactatccga acaaaaggtt ttgtttgttc atgctttccg
     3601 aaattcttta attccattat tgacacttat ggcaaccatg ctacctggtc ttttaggtgg
     3661 cagtgttata gtagaatcta tcttttcaat acctggaatg ggtatgctgg cattcgaatc
     3721 catactttca cgagatattc ctattatcat ggctataacc tcaatttcgg cctttttaac
     3781 gctcattggt attttccttg cggatattat gtatgcaatt gccgatcctc gtatacgact
     3841 ggaggctaaa caatgaaagg ctactggtca attgtatgga aacgctttaa acaaaacaaa
     3901 ctggctgtag ttggcctctg tattgttatt tttttatttg taattgcatt atgtgcaccg
     3961 ttaattgcca acaacaaacc ttatatttta atttacaaag ataaactata ttttccaatt
     4021 ctgtttgaat atcctgaatt ttcaggttta gattttaaaa aaatcgaggg attcaaaata
     4081 tttccaccaa taccatattc atattctgaa tatgacctca atgcaatcgt gctgccaccc
     4141 agtgctaaac atattttagg aactgacgaa cagggtagag atttggctgc acgtatgtta
     4201 tatggcactc gtgtatctat ctttgttgga tttgtagcag tattcattta tgtatctata
     4261 ggtataatta ttggtgcctt agctggattt tatggaggat gggtagatat tattatctca
     4321 cgtattatag aaattgttat gtgtttccca acgttttttt taatccttac tatcctggcg
     4381 ctatggggtc agagtctgct taacgttatg attgttattg gtataacagg ctggacaggt
     4441 attgcccgta ttgtacgtgg tgaatttctt aaactacgtg aacaggattt tgttattgct
     4501 tcaaaagcgc ttggtgctcg agattactgg attattttta aacatatact acccaatgcc
     4561 atggcacctg ttatggtatc agcaacattt ggtatagctt ctaccatact cattgagtcg
     4621 tcattaagct ttttaggatt tggagtacaa ccaccaacac caagctgggg tgatatatta
     4681 tcacaatcac gggatttcat tgattttgca tggtggttga ctttgatccc aggatttgct
     4741 atattcataa ctatcacatc ttacaattta gttggagaag gattgcagga tgcgcttgat
     4801 ccaaaacaat atcgctaaaa ttgtactcat actatttata tcaaatacta ttgcatgtct
     4861 ttcctttttt gaagcaaaac aggaaacaaa tgacctctta cccggtgaaa cagaattata
     4921 tacctggaaa ctggtaaaaa gtattactgt tactaataat gatgctatcg ggaacatttc
     4981 acctgattat tataaatact ataacgtcat ggaaatagtt aaaggcgatt atcagtcaat
     5041 taataacagt tcattttttt taacagtaac tatcgccaga acagtaacag ttgatgatgc
     5101 ttatgggtta tttacacgtg aaaggattta cgtggaaaaa cctttctata aaaacgatta
     5161 cagcatatac tggaaaaata agagtatcgc atttaaagga aatatttttc taaaagttga
     5221 aacgaacgat cttgatttta tacaaactca aaaaagcata attgaaacaa tactgcaaaa
     5281 aatccaaaat gctcacgcca tacctgatta tgttgtcagg atatcgaaat attgtgacat
     5341 cgatagcata atactattca atgaaggtat tccacaaatt ccatatttaa aaaacattgt
     5401 ggttgggaaa aaaattatag aatctaaaga tatcatggta ttttttaaaa tttattctac
     5461 aaatattgat gcatcacaaa attttgatgc aattttaaaa aacgatagta cactgatgat
     5521 tttagaatca ggtaaagtaa aaatagcttt taaaaaatgt ggcgatagtt actgttatat
     5581 agcccagtat aaagaatgga tatttggttt gtatgacatt gttgactata atgcgggtaa
     5641 acaatacatt aatactcttt ataaagaatt gagcaatcca taaatgtttt atattattgg
     5701 tgacatacac ggatattata acaaattatc agggcttttt gaaaaaataa agaagcatat
     5761 tactgataat gatacaatca tttttttagg ggattatatt gaccgcggaa aacattcttt
     5821 tgaagttata gaatttttaa ttgatttaca acaaagctac aacacagttt atttacgtgg
     5881 taatcatgaa tccatgtttc tggattatct gcaaggtaaa gaaattccat tgtatatgta
     5941 taacggtgga caggcaacaa tagattctta tatacaaatg atgggttcat tacgattgcc
     6001 agaagatcat aaagcttttt tcaatagttt aaaattatat tatgaaactg atgattttat
     6061 tgctattcat gcaggcatgc ggccaggtat accagttgag ttgcaatcgc cttatgatat
     6121 gatgtggatt agagaagatt tctatttaaa acagtaccga tggccgaaac tggtagtatt
     6181 tgggcataca acaactgtta atattacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnatt catgttgata tttttacatg caggaataaa tactatgatt tttcgtattt
     6361 tcaatatatg attgtaatgt cgattattaa taataaatct atgtattgtc catgataatt
     6421 ataaaggaaa catcattatg aatatatttg aatggttaaa caagaaattt tcgtatacat
     6481 ttgcattatg tttttttact atttttggtg gatggatgag tgcagtattt ggatattatc
     6541 ttggcacatc atttatactt tctgagtata atgaaatgca ttcacttgct ataaagtggt
     6601 tgccgttagc tgtgcttatt ccaactttac tgcattatat aacatttggt tttttaaccc
     6661 ctttaggaat tcctgcattt tttaaatctt tacgtgatgt taataatgca tttaaggggg
     6721 ggacactcaa tccatctctt acagatgaga atctccaggt attgtatatc caattgtcac
     6781 atcttccgat gtacaatatg gttgcagcat cactatttgg tacattgtgc ggcttttttt
     6841 taatgggatt tggctactat gacatgatta cacatggtac ctttaccata attaaaatga
     6901 aaattggtat caagatagta accattggtg ttttagtagt tgttgtatta tatgggatgt
     6961 caacatattt attaagtgaa attattacca atccccacag agcactggta tatcaggaat
     7021 tacgtcaacg tactattaat atttatcctc gtggattaat tggtttgcgg attaaattta
     7081 gctttttcat catacttatg attataactc ttttaacgtt tgctgcaatg atggaacagc
     7141 accggttata tgaagaaaca cgatatataa atattctaac atatttctta gtttctattg
     7201 ttgctggtgt ttttctcatg tatataaatt cagattcggt tatgcgaatt ctggatgaaa
     7261 tgggtattgt tacaaaaaga atttcctcag gacaggaagc atggttcaga gtaatgtctt
     7321 atgaaaggga gtttgccgaa atagaatttt caatcctgga gatggcaaaa gaaattgaag
     7381 aacacagaaa aaacttagag ttaaaagttg aacaacgaac agaagaactg cgcgaagcac
     7441 tggcaagcct taaagaaaaa gatgacatga ttcagaaaca attagagata gctagcaata
     7501 tacagcgcag tatattgcca ggccgtatcg atgactggaa tgagcttaaa ttttcagtac
     7561 gatatattgc tatggaaaag attggtggtg acttttatga tgtatatcag atgaaaggag
     7621 ataaattagg aatactggtt gctgacgtat ctggccatgg tataccagct gcacttatta
     7681 cgtcgatggc aaaaatttca tttagttctg caactcagca atatgattca ccaaaaaggg
     7741 tattccagga agtcaaccaa aacatccttg aacatatcaa aactcaggat tatatgactg
     7801 ctttttttgt ggttatagac gatgattata atgttaccta tgctaatgca agccatcaaa
     7861 aagcaatttt gatacgttct gatgaaggca ggacagagct tcttgatacc aatggattat
     7921 ttataggtgc tattgaagaa gctagagaaa cgtatgaaga aaaagaaaca aaattaaaat
     7981 atggtgaccg tctattacta tatacggatg gtatacctga agctgccaac atagagcgtg
     8041 aagaatattc tgtggagcga ctacaagaag ttgcagtaaa aaacaggcat ctttcactgg
     8101 aagattttac aagctttata attgaagatg tacaacgttt caaaggaaaa gctcctgtag
     8161 aagatgatat aaccttactg gttattgaat tggcaaggga tgaagctgtt gatattataa
     8221 aacaggcaaa aaaattaatt gatgaacata aatattatga agcaattgat tatttagaaa
     8281 gaagtttagc tctctatccc aataatcgca aacttatata taatttagca aaaaactatt
     8341 tcagagtaaa taactttggc aaggcaactg aattgattga acaatattta gaaatggata
     8401 aacgaaataa atatgcatat tacgttggtg gtgcagctta ttatcagatg atgaactatg
     8461 aaaaagcaat acaattgctt gaaaaagctc aaagtttgga ccccaacttc accaatgcat
     8521 tatttgcatt aggtatggcc tataagaaaa taggcatgta tcaggatgct ttacagattt
     8581 ttgagcgcgt tgccaatatt gaccccgata ataaaatggc actatttgaa atcaatgaaa
     8641 ttcgtaaagg gttggcctaa ttattggtct tactgtgtaa tatttattgt tccatctggc
     8701 attatatgca tatatagctt ttctattttc gcagagtaat tgtaattttc atctggctga
     8761 tatagtttca ttacatcatt aacgtatgta atagttgatt ttatatttgg aacagccata
     8821 ttttttctta caattcctaa acctgcatga tacgcagcta aagccaaaac aatatcattt
     8881 ttgaaaacag ataataacga cttaaaatgt tttacaccag cttgaatatt ctgatgagga
     8941 tcaaatgggt cctgtacatt atatgcctgt gcagtttcct gcataagttg cattaatccc
     9001 atggcaccag atacagaaac ggcatcttta ttgaaattgg attcaacttt aataatgcag
     9061 cgtattagct gagggtcaat attttcaatt ttacaaatat taataataat tgtatcatag
     9121 ggattttttg atagatcaac tttggtgatt ttagtctttt tattatgctg aagattataa
     9181 tattcaatgg tattatcatc tgttttctta gatttaatct gagcattagc atgaaaagaa
     9241 attaagtcat ttaaaataat gcatgttaca aaaataacaa taaccacttt actaattatt
     9301 ttgagaaata gtattatata actcttcata ttcttttgcc gtatcatccc agcttctttt
     9361 aaataacatt gcattattaa ttaatttatt cataataact ttatttttat atactctcag
     9421 tgcatgacga atggttgtat aaagttcagc actatcatta tttttatatt taaatccatt
     9481 gccagttttt agtctttcat caaattgttc aattgaatcg tcaagtcctc ctgttgcacg
     9541 tactatagga atagtaccat atgctaaact atacatctga ttcaaaccag aaggttcata
     9601 acgagaaggc ataacataaa aatcgctacc agcttctatt aagtgtgcaa gtttctcgtt
     9661 atatccccac caaacaccaa ttctatcagg aaacattgcc cgtaatgcct caaatctgtc
     9721 aacaatccac tgttcgccac ttccaacaat aacaaattgt atatcttttt catcaaaaaa
     9781 aagccaatcc agtgtattta taagaatatc tatccctttt tgatatgtca aacgacttat
     9841 gcatcctatt atgggaacat tgtcattttt taatccataa aaatttttta gagctttttt
     9901 gcacttttgc tttccagtta gattatgaat tgaatactta taagggatga aatcgtctgt
     9961 ttctggattc cagtgttcgt aatcaacacc attaagaata cccgatagct tttgattata
    10021 ttttttaaat attggtgcaa gatcatatcc atactcagat tcttgtattt cctgtgcata
    10081 ttttttgctt acagtaacaa gattatcagc attaactaag ccagccttta ataaattaac
    10141 cttgccaaaa aattcaatat tttcaggatt aaataattct tcagagagct gcattaatcc
    10201 caatacctct ttaccaaaca ccccctgata gccaacgtta tgaatagtga gtattgattt
    10261 tgcttttttt agtacataat catttgcata gtgtgtatta agaaatagtg gaatacacgc
    10321 agtttgccaa tcattacagt ggattatttc cggaactatc gccaattgtt ttattgcttc
    10381 aatacaagct ctgcaaaaat atataaagcg ttcggcattg tcattgtatg atactccatt
    10441 gtagtcatat aaaccatcac gtccgtagta atcatcatgc tcaataaaat atgtatgtac
    10501 accttccaga taatcagtat agaatatacc cgcccagcgg attccgtttg ctattggtac
    10561 cgctaagggt ttgccaatta atctgcagtt gaatctttgt ttatcaacac aataataacg
    10621 aggtaaaaaa agatgaactg tatgcctgcg ctttgccaat gcttttggca gtgaatacga
    10681 ataatcagca agacctcctg tcttggcaaa ggggtaagcc tcacttgtaa taaacacaat
    10741 attcatatta ttttgtaagt gaaaccaatg atggataata ctcttcaggt gagtggaaac
    10801 ctaataacgt acagattgtt gcagcaacat ttgccagtcc ctgttcttta tgggttgtga
    10861 gtgtatattc accattatac tgagaatcat aaataataaa tggaactgga tttaatgtat
    10921 gcgatgtttt tggctgtggt ttacctgtct ttttatcaat ttttattgtt ccagttttag
    10981 catctttttc atacatctca tcaaggttac catgatcagc agtaatgatg gcaatagctc
    11041 cggtattgtc tattgcatgc agtagtcgcc caatacacaa atcaacggtt tcagcagcaa
    11101 ttatggctgc ttctaatatg cctgtatgac ctaccatatc accgttagca taatttaaac
    11161 gaataaaatc gtatttatta ctgttgattg catcaattac agcatcggtt atttcagctg
    11221 ccttcatcca tggacgctca ttaaattgca ccctgtcaga gggtatctcc tgatacgttt
    11281 cgtagttttg atcaaacatg ccacttctat taccattcca gaagtacgta acatgaccaa
    11341 acttttgtgt ttcagatatt gcaaattgtc tgcaaccgtt atgaaccaaa tactcactta
    11401 tggttttatc tatagaagga ggaaatacca gataattctt ggggatatga agatcaccat
    11461 catactccat cataccagca aaaactatat ccggaaaacg ttctctgtca aatttattga
    11521 aatccttttc ttcaaaagca cgggaaattt cgatagctct gtcaccacgg aaattgtaca
    11581 ggattaccga atcaccatca ataattggcc caactggctt gccatctttt gcaataacaa
    11641 atgaaggcaa atactggtcg gttataccag gttgttcttt tctatatgtt tctatggctt
    11701 cagtagcaga agtgaatgct cttcctttac ctaagacatg tacatcccag ccacgtttta
    11761 caatattcca gtcagcttca tatctgtcca tggtggttac cattcttccg ccaccagagg
    11821 caatacagaa atcaaaaccg ttttgattaa aagtatgcag aaatttctct aagctatcaa
    11881 catattgcaa tgctgatgtt tcaggaacat ctcttccatc taataaaata tgtacacgga
    11941 ttttttttac tccttcttta gcagcctgtt caatgagttt aaagaggtga ttaatatgcg
    12001 aatgtacatt accatctgat agtaggccaa taaaatgcaa cgttgttcct gtattaactg
    12061 gtttttccag taatttgtgc catatttcag ttgaaaatat ctgtttagat tcaatagctt
    12121 tatcaacaag ctttgcaccc tgatcaaaaa tcctgccagc acctagcgca ttgtggccaa
    12181 cttctgaatt gcccatatcg gcatctgatg gaagtcctac tgcagtccca tgtgctttca
    12241 atgttgtata ggggcaggtt tgcattaatt tatctaacac aggagtacgt gcaagaaaga
    12301 aagcattacc ttcatcatgt ggcccaattc caataccatc cattattatt aatacaagag
    12361 gtcccttcct tctgctaaaa ccgttgggca ataatttcat ctactcctcc agaagaaatg
    12421 tttcaagtga agctaattct ataacccgtt ttacataatt attagcattt attattctga
    12481 actttcttcg attgttattg aaattgttat acaatgtcat aaagccagct aaaattgtcg
    12541 aatcaacaac ttcaacattg gtcatatcaa ttatagcatc acggctgtca ttctgtaaaa
    12601 aattgtgcat ttcttcaatt aattccttca aagttcttgg atttaattgt tcactgataa
    12661 taaatatgca tcggttttct tcttcttttt tcgtaaaaat catggcttac ctctttaaac
    12721 tgtaatattg tattttttat aaaattcatc ttcggtaaaa atgtttattg aatatttagt
    12781 caatgctctt tcaagaatca tatatatttt atcattagca gcataaaaaa caacatcaaa
    12841 attatgttca tcaccatatt ttaaaagata cataatagct gcaactgctg cagaatttat
    12901 ggtatcaacc tgtgaaaagt taattgcaac agttgaaatt ttttcaggga tctgcttttc
    12961 aagtaatttt attaatttca catcatcccc atactcgcca ataccggtaa cagttactat
    13021 aattaaatca gagtaacgag tatgtgtaaa attcatttac ttctcccaga ttcttttatt
    13081 ttttttaaaa tattttccaa ttgttcgata gctcttatag aattgtccga tagtttaatg
    13141 aaatcatcac catagaaata atccttcatt aaatattcaa ttagttctgt atatgttgta
    13201 tttattaatt ttactgattc ttgtaatgtt tgtttgcgaa ttccctcttt ctttaaaaaa
    13261 ggaaatataa gatataaaca tgtatacata aaccttttta taacttcttt gtaatatttg
    13321 ataatgagaa acaggatata tgggaataaa ataataccat ttgttttcaa cattgtactt
    13381 atagtgtata gataatcaat ttcaaaacta ctgttccatg taaaccacag tatatttatt
    13441 attatcatgg aaataaaaaa ggatgttttt ccttttggaa aacctattgc tgacaaaccc
    13501 gctgatgtga aagcaattgc aaaaaaaagc gcgatagctc tgtaacctaa aagatttgaa
    13561 attgcagata taaaagaata aatattatcc aggaagcgtg ataatccatc aaaagttgtt
    13621 tttatctgat tatatgtatc gaatatctct atagcataca gtggaatggt gttaaaaata
    13681 tataaaagta caaataatga tatcaatata ataaataatt tcttatcaaa tataaatatt
    13741 ttatcttttg taagctttat catgctatat tcaaatacga tatattttaa catttctcac
    13801 tggtttatac attttaatct tataacttat aatttgtcaa atattttata tgtcaaaaca
    13861 aatcaaacaa tcttttgaac agtcccagtg tatgcaccat attctgtgaa aaaattactt
    13921 gccaataatg tatggttata ttttctttaa aaatggtatt atactataaa ttattatatt
    13981 ataactattt aaaaggtact ttatggatga tttaattaaa accgctgcaa aaattgttaa
    14041 ggattctata tctactatag cattaacggg tgcaggcatc tcagttgaaa gtggtatacc
    14101 tgatttccgt agtgcgggtg gattatggac acggtataac cctgaagaat atgctcatat
    14161 tgatggtttt agaagaaacc cgcgtaaaat atggaagatg atatttgaac tcatggacat
    14221 gacaaggaat gctatgccta atccagctca tattgcactt gctcaactgg aagagatggg
    14281 cttattaaaa gctgtgataa cacaaaatat tgacaatctc caccaaaggg caggtagtaa
    14341 aaacgtaatt gaatttcatg gcaattctga acgattgcaa tgtatttact gtggtgcgga
    14401 atatgcacct gatgaatttg ttatcgaaaa cgagcctcct caatgtaaaa agtgtggtga
    14461 aatattaaag ccaagcgtgg tattttttgg tgagatgatc ccccataatg cattaatgga
    14521 atcacagaat ttagccagcg atgctcgtgt ggttcttgtt attggaacat ctgctgttgt
    14581 atatcccgcc agcagtattc cccatatagc taaagctaat ggggcatata ttattgaatt
    14641 taatttagaa aaaactgtca ttacaccaat gataactgat atttttattc aggggaaagt
    14701 tggtgaaaca ttaccaaaat ttattgaagc cataaaagct ctgcaggctt aattcatgga
    14761 gctatcgcct aacatacaac aaagaattca gatattaccc gatgccgtaa aaagaaaaat
    14821 agctgctggc gaagtaattg atggccccta ttcagtaatc aaagaacttg tggaaaatgc
    14881 tctagatgcg caagccaccg atattgatat tgaaattgaa gaaagcggtt taaaacgcat
    14941 tgtggtaaga gataacggca tcggtatcta ccgggacgat ttacctttag ctgttgagga
    15001 acatgccacc agtaaaatca aaagcattga tgatattttt tcaattatta ccatgggatt
    15061 cagaggggag gctctttcca gtattgcaga agtatcacac tgtactatat tgtctcgcac
    15121 agccgaagaa gaaattggtg gaaagcttgt aatagatgaa ggcaaaaaag aattattcga
    15181 ctgggcaggg ccaactggca caacgattat tgtggaaaat ctttttttta acacacctgc
    15241 ccgtaaaaag tttataaaag ggctaaagtc tgaactcaat cgaattaaag atacaatatt
    15301 tgcactagct atagctaatt ctcgtataca atttaaagtg aagattgata ataaaattgc
    15361 atttgttttt gaaccagctg atactttgca aaggattgct cagatatatg gcaatgaaat
    15421 ggcagataat ttgctgtatg gttcgttaag cgatttacac tgtactattg agggctattg
    15481 cactaaacca aactattacc ggtcaaacag gtcattacaa tttttatatg tcaaccatag
    15541 accagtccag tttccatatt tcaattttat tttacagcaa gtgtaccaaa ccatattaca
    15601 aaagggacaa tatcccgcag catttatttt ttgcaatata aaacccgacc ttattgatgt
    15661 caatgtccat ccggcaaaaa aagaaatacg cttttttgat aataattata tacattcaat
    15721 gatatataat tttgcaaaca agctcataag tggaaccata cacgcatttc caattaaaca
    15781 caatgataca caattacaga ataacaacat taccacagca tctaatgcaa ataattatac
    15841 agataataat acaccacctt tactatatac acagccaaat gttaatcctc aaacgttaat
    15901 acatgaagta cagcatctgt atactcaatt acacaataaa gattattctg taattggaac
    15961 tatatttggt tcttatgtac tgattcaaaa agaaaataac ctgtatatta ttgactttca
    16021 tgccgctcat gaaaaaaaat tatttgatta tcttatgagt attgattctg tttcagcagt
    16081 acagcaactt attatgccag ttgttatgga attgtcgcct gatttgctta ttcatataca
    16141 ttcctacaaa gatattctca ctaactacgg aattatcata gaccaatttg atgaacattc
    16201 aattgttatc aatgctatac ctgatttttg caataccaat gatatagaag gattaatgag
    16261 agatattgtc gatagtttgc atgaaaacaa atattcttta aataatatta aaattaaaat
    16321 aattgaaaaa attgcttgcc attcagcaaa acgtgctaat gatataataa cacaggatga
    16381 cattattgca atagccgatt atgtactttc aacaaatgac taccggtgcc ctcatgggcg
    16441 accatttgtg tacatagtat caaaatctga attagagagg tttttcaagc gatcatgaaa
    16501 caaaaaataa ttttaggaat ttcttcatca attgctgcat ataaagcctg tgatctggca
    16561 cgtttgctta ctaaagatgg atatgatgta ttctgcgtac ttacagagaa tgccacacat
    16621 ctggtagccc cattaaccct gcaaacactc accggaaacc cggtatacac cacaagtttt
    16681 gccatacaat ggcgcgaaat gggccatatt gaattaaaag aaaacgctgc gttattactg
    16741 gttgcacctg caacagcaaa catcattggt aaatttgcca atggtattgc tgatgacctg
    16801 ctcagtacaa catttttagc tgttaattgt ccagtggtga tagctcctgc catgaatcct
    16861 aatatgtgga aacacaaagc tgtacaggaa aatattaaaa aattaaagga atggggctgt
    16921 atatttgttg aacctgaaac aggacttgtt gcttgcggtg atgaagggca gggcaggttg
    16981 gcttcaatag aagaaattta taaggtagca attaatgcaa ttacaagata aaaagattat
    17041 agttactggc ggtccaactc gtgaatggat tgaccccgta cggtacatat ccaatgcttc
    17101 cagtggcaaa atgggaatag ccatagctga tgctgcatat aaacaatgca aagaattagt
    17161 ttttattcat ggacctattg atgaatcact catcagcgga aagccatatc gatgtaaagg
    17221 tgttgaaagt actgttgata tgcttgaagc aattaaacag gaactatcgc ccaatatggt
    17281 attgataatg gctgcagcac ctgcagatta caaacctgta actaaatcac ctacaaagat
    17341 aaagaaaact aatgaaaacc tgacaatcga actacaacgc aatccagata ttttaaaaac
    17401 aatagccagc ataaaaaagc aacaacaaga gtttaataat attgttctga ttgggtttgc
    17461 agcagaaaca actgatgtga ttgcgtatgg taaaacaaag cttgttgaaa aagaactggc
    17521 aatgatctgc atcaatgacg taagcagaag tgatacaggt tttggtagcg attttaatga
    17581 agttacggta atgcttgcaa atggcatgat catcaacctt catagaatga caaaaaaaga
    17641 agtggctgaa ggcataatca atattattat taatgaatta tagtaatatt gtttttttat
    17701 gtttttctaa aatcttgctg ttacatacac catcataagc gatagtacca tgttccagaa
    17761 caataagtct gttagctaaa tgttgtactg cataggctga atgtgtggca aaaatcattg
    17821 gcatgttaag atctgataaa atatgtatca ccgcatctgc cgatggctta tctaaatttg
    17881 ccaggggttc atcaagcaat agtatttttg gattttttgc taaaacactt accagagctg
    17941 ctcgtttcat ctgcccactt gaaagtgcca gagggctgtg attgagcata tcatccatgt
    18001 ttactttttt tagccactga agtgcctgtg attttttttc ttcttgtgag ccttcacgta
    18061 tgctcaatat cacatcattt aacagatcag gcatgatgaa ttggtattca acctgtgaaa
    18121 atactatgcc gatggatttt ctaacctgag atttattacg tgctgttaat tgcatatcaa
    18181 aaattgtgat agtaccactt gttggcaata gcaatgcgga taataataat agtaatgttg
    18241 attttcctga accattatga ccaataatgg caaccttttg gttttctgaa acaattaacg
    18301 atatattatg tagtgctaca gtcccatcag gatactgata tccaatactg tcagtgttaa
    18361 ttatattcat tttatattat aacccctgat agatacaatt tacaattaat agtataatga
    18421 ttacaccgct agaaatatac tcattaatag aatgtttatt gtgaataatt aaatttggta
    18481 ttgtactgat ggttcgtgag agaataccat ttctataata tgcaagtgca taaaactgat
    18541 ttgaaaaaaa agcaatggaa taatatttgg gaatgtaata cttgtctttg gtttttaaat
    18601 ctattcgtga ttgaatctga aatacaatca tcctgtttaa ttttaataaa gtatagagta
    18661 ttcgtataaa aataagaaaa aataatttta atgaataagg gagtatattt ataaatgctt
    18721 taaaaccatc tgaaccaata aaaaagcggg atccaataat aattatcgtg atacatacaa
    18781 tcttaaaagc tgtgatactg attattacaa aatccagttg ttcgttcata agacctgtta
    18841 aaagctgcat tacggctatg gtaataacca gtggtataat catcaccagc gtatatcgct
    18901 gttgcttagg tatatacatg aaataaacca tacaaataac aaaatatgat gctatccata
    18961 aaacagatac agtagtaata accgaacata gtataatcac actaattgta atgattattt
    19021 ttatccattc aaaaatttgc attattctac tgtagcatga ataaaattta attcaggctg
    19081 gtcaatatat tttttcatga tgaaaccggc ttcacgtaac attgtatcca gttcttcacg
    19141 atacggcata taatccctgc taaccggtgc attaagatta tggtggaact cattcagtgt
    19201 cttagtattg gataggtgaa atatatataa tgacccatta ttttttaaac attcatgaca
    19261 tttttttaac gctttcagct tatcttcaat atgcggaaaa actgcaaaac tcagaacaac
    19321 atctatacta ttatcaggta atgttatatc ttccaatggt gctactatat attgtatatt
    19381 tgtaaaatgt ttatatcgct ctttagcttc ctcaatcatt ttgtcagaag tatccacagc
    19441 aattaatgtt ccctgcgggc caatatattc ctcaattatg ggaaaaagca cacctgtacc
    19501 acaaccaaca tccatgacac gtgaaccata tgtaatatct atcattccaa aaatattttt
    19561 aattttttgg atacgcgttt catcgttgcc aacaatctca tcccagttct tgctcaaggt
    19621 attgaagtat tctattttat catcatagtt tttattaacc atatctatcg ccctgcattt
    19681 ttttgtatta ttacattaat gccatttact gcagtgggtg ttatcaatat aagcagtatt
    19741 atccctggaa aacttttaaa caagtcatac aggctgaaca ttccaaatga tatcttaaaa
    19801 taaggaagta caattgcata caatacatat aaaattattc tatcaaaaat aattgcaaaa
    19861 acaataataa gatagttgtt aagataaaac ttttgtttta ataatgaaat gaaaaaacaa
    19921 aacaatgaca attcaatcat catgattatg gcaattggcg ggtagagtgg tggcatcccg
    19981 gtcaatactg ttgaagttat tggggcgata gttcctgtta aaagagcaaa tgcaggcgta
    20041 aggaaataag ctcctattga cagtgggatg aacattggta aaaatattga tccaagacca
    20101 agaagatgaa ataaaactgg tattactact gcacaggcag cagttattgc tgatataatt
    20161 atctgtgtga tatgtaactc atcattcatt gtatacgatt tgtaatgtat gatatttttt
    20221 ctttaatttt aattatcatt tgaaaattac ctgtaaacat tgatttattg taacatttaa
    20281 acagtaaaat ataattggaa taggtaaccc acttataatg ataaaaattc ttttaaagaa
    20341 tattagtgta atatccctgt atgccatttg ttataatgtg taaaaagtaa ctcaattaaa
    20401 taccaaaaat attactaaac taatttttgt aatacgcatt caatagagat tttttgcaag
    20461 tatatttttt tgattcgaat ataaaatttt tcttgaacta gataggtttt tggataagat
    20521 agtatttaga ggttgaacca ctgaaccact taaactagga gaaaaattat ggcaaaaaaa
    20581 ttcatacctg attggactga aaaagcacca gaaccatatt catatcgttc aatttttaaa
    20641 tggggcaatc ccgaaacctt taaacatcca aattcaaaat tatatcagga attaaaacaa
    20701 atatttcaac ttgatgatag ttatttccag cataagacga atgaggggcg acagcaggtt
    20761 caatataaaa aaccagtaaa cttaactcaa aaccagatta aagcattagc tgaaattgtt
    20821 ggttatgaca atatcagtac taatgaatat gacaggttaa aatttgcaac tggcaaaact
    20881 ctagaagaag cattgaagct tagaaaaggt attatagagc gagtagctga ttgtgttgta
    20941 caccccagga atgtagaaga tgttcaaaaa attgtagcgt attgtaataa gcataaaatt
    21001 ccaatctatg tatatggtgg tggctcatca gttaattttg gattacggcc tgctaaaggt
    21061 ggtttaactc tggtattatc tactcacatg aataagatac tgaatgtcaa tgaaacaaat
    21121 caaactgtta cggtacaggc ggggatactg ggtcctgcgc ttgaagaagc attaaataat
    21181 gcacaaaaac tatttggtac aaaatataat tacacctgcg gtcatttccc acaatcattt
    21241 gagtattctt cagttggtgg ctggattgtt actcttggtt caggccagtc ttcaagttac
    21301 tatggtgatg cgtatgatct tgtaatcagc atgaagtggg ttactcctgt tggaacgttt
    21361 acaacgaaag attatgcagc aactgcaaca ggtcccaaag tgaatgatat tatgaaagga
    21421 tgcgaaggta cgtttggcgt gcttgttgaa gttacctgga agatatttag atacatgcct
    21481 gaaaaccgaa aaagatttgg atttatattt aaaacatttg aagacgcaat taatgcttct
    21541 cgtgaaattt cacagggtga atttgggatg cctgcagtat ttagaatttc tgatgaagaa
    21601 gaaacgcata ttggtctaaa gttatacggt attgatggca caattttaga tacattcatg
    21661 caattgcgtg ggtacaaacc catgaagcga tgccttttta taggttcagc tgaaggtgaa
    21721 aaacattttg ccaagaatat taaaaagcag gttaaaaaga tatgtaaaaa acatggaggc
    21781 atgtatatta ccggtttacc aacacgcatg tgggaaccag gccgttttaa agatccttac
    21841 ttgcgtgaag atttaaatga ttatggtatt cttattgata cccttgaaac aagtgtcaaa
    21901 tgggataatc ttcatcatgt tcataaagct gtacgacaat ttataaagag caggcctcaa
    21961 actgtatgta tgacgcatgc atcgcatttt tatccacagg gaaccaatct atatttcatc
    22021 tatatcatga aaacagatga tattaatgaa tacattcaat ttcaggatgg tatcattgat
    22081 accattgcaa aaagtggtgg ttcactgagc catcatcatg gagtaggtcg gatgctcgct
    22141 ccatggatgg aagaacatat tggcaaagaa cagatggcag ttctacgtgc aatcaaaaag
    22201 cattttgatc caaacaatgt catgaatcca ggcggtcaat taggactgga tttgaaggat
    22261 aaaaattggc ggaaaataaa atagattaca acaatatatt taaaatattt aggggcgaaa
    22321 tttaatccgc ccctattatt ttattagaac gtatggttaa agtttactga tatagaagta
    22381 tcttcttcac tcacaccata gtagaagtga ataattgttg ccatattcca agctatgact
    22441 aaaccaccac cataaccttg atggtaatca ccccagcgtg gctcactgaa aggatcaccg
    22501 gctttgtcat aaacattacc gacatcataa aatgcggtca atttaaactg gaaattctga
    22561 ttccagggat ttgcttcagc aaacttccag cgcaattcag tattagcaac tgtcatggtt
    22621 ggtgcaacaa agcgctgttc tttataacca tggattgtac ggttactgcc attaccagtt
    22681 cttctgttaa gtgaaaatcc aaaataaccc atttcaaaga atggagcatc catattggta
    22741 tcagtgtaac ctgcacgcaa tgcaagtgtt aagcttgaaa gaagtggtat gtagtactgc
    22801 aactgaacag tatttcatat aaaatcataa tctgaaccca accatcctct ggaaacttca
    22861 aatgcatagt caatgaaata acctgatttg ggatccggtt catagtcacg ggtatcatag
    22921 tatacaccaa tacgtgcaaa gtttgaccaa ccacctttat aaccctcagg tttccactgt
    22981 tctagtaatg ttggcccctg aacatcacca tcaaactttt caccatccca tgtatcaatg
    23041 gtaactttct ttacttcaaa accaaccagc atcttcaaat tctgtgtcag gtcacggtaa
    23101 atattcagga agtagcttgg ctttgtatac tgataattgt aatatttttt aaatgcttca
    23161 ttatcatcag catagtctaa ataatctttg tatgtatcaa attgtatggg tggattacca
    23221 acacctattc catcagtatc aagtgttaat ttttgttttg cagcatcagc accaatccca
    23281 aagaagtttg cattgatctt tttatcatac gcaactgaag taataattct gaatttagtt
    23341 cccatgatat agggcatatc caggtttact tcatggtact ggtacccatt tgttgtctga
    23401 tagaactgtg catacaattg cattttatag ggagcatagg aaaagtactc ttcatccctt
    23461 gtaccattgt tataaagata agcccgcacc ccatatccaa aaccattgtc actgtcatag
    23521 ttaagcaatg gtaaacccgt tggataccag ccttctttct tttttgcaac atcttcatct
    23581 gaaagttttg cagcaaatga aggcatagca atagccaacg acattattaa aataagaaac
    23641 gttaaaaagc tttttttcat tggcttactc cttttaaaat ttttaaagaa tattttaaac
    23701 aataagattt tcaatacact attgttaaaa agtcaagaat attttactat ttttatcgtt
    23761 ttcgatatat attcagttat ttttattatt taaacataaa tgaagcttct tgttccatgt
    23821 aaattaatat aaatgggata gatttgttat tgcttcgtaa ttctttttct tttataataa
    23881 ctttttgtgg aaaatactat agatagtgag ttttttatac taaattttat tataacctga
    23941 aattatttaa atgcataaac aaccattgaa ctggtaagtg caaggtttcc tatgtgtatc
    24001 ttgcttatat aatgatatgt tgctatccgt agccagtttt tataatcgtt tatactttta
    24061 aaattcattc ttgatttaag taattttggt aacaatccaa agtcaactgg ttgataaact
    24121 gtaacgtgtc taaaaccaat ttgtgttaat gtttcagtta ggtttttttt cgtaaaatac
    24181 gaaaaatgcc cgggtaaaaa ataaccatac ttttgcttct gtaattttgc ctgcaaacca
    24241 gccatatttg ctgtttgtat caccagcaat cctttatctt tacacaaatg gtaacattca
    24301 gttaaggctt cacggggatt tcgtatgtgt tccagaactt caatcatagt aattactgaa
    24361 aaagtatctt ccttaaatgg atggtcaaac aatgtgccaa catgaataac atcattgcta
    24421 aaccgttgtt gggcatattt gcctgaatac tctgatggtt caatcccata aaccgtaaag
    24481 tacctggaag cactctgtag aaagccacca aatgaacaac ctacatccag taaattccct
    24541 gattgaacat attttctgat agtttttaat cgagcgttcc atacatactg agagaaatgc
    24601 tcaacatctc tttcatcata gtatgtataa ttggcttttc ccgtataata tccttcatca
    24661 tataatgcca gttcactttt tttggtaagg ggtggattca tgaagatgaa tttacagctg
    24721 ttacactgat atacagtaaa agatggttga gtatgaacta tcgcatataa aaaaaagatt
    24781 ttattacttt tgcatagggg acacgtgttc agtttgcgtt caaattcaaa tgtttgtttc
    24841 atattgtagc gtaaaaatac cagtgagata atgttttagt aaggtcatcc agaacagttc
    24901 gttccattaa accaatatga tattcattaa aaacgtgtaa atatgtttta acttcatcca
    24961 acgagaatag cgaaactact gcgccattga tatcgggtag ggcagtttcc caggtattat
    25021 tacccaggtg tctgccttta gctataaagg tatcattaat actgcgcagc gttccaaaaa
    25081 atactcctcc agggcaaagt attcgcttaa tctcttgtaa catcacaggt agattatctt
    25141 ttgttgtgta atggagtgat ccccatgaaa caactatatc aaatgttctt tcgctaaatg
    25201 gaagttttaa agtatttgcc tggacaaaac ttccctgtaa gattttgtta cagtgtgcaa
    25261 gtgcattaaa acttatatca atgccaacaa cataatcaca gccaaactca tgtaaaagtt
    25321 tgacatggcg acctgtaccg catccaacat caagtgccct ttttttattg cctgcatatt
    25381 ttgcaagcat tcgtacaagc tgttcatcag gatataagag cacagaagtg tcccgggtat
    25441 aatgtttatt ccagctttgt gttgttgcat ccatggttat tcaatgatga tgtgcatttg
    25501 ttcaagtaat gtgtcggctg tttgcaaagc tttttcaact gtttttccct ttgtaataat
    25561 aacacctaaa cgttcatgat tattttttgg gtaatacagt atatcaccgg gttttgcaaa
    25621 catctctgca tgaattaagt gattattttt tggcatagaa taggaaacca acttaccatt
    25681 gcggcccatg atatatttta caattgcaga tactgaagat ttagggggta cgtttacttt
    25741 ccctgtaata attgcctgta aaaacaattt gaaaacatta cttttcaaac gtgcaggaat
    25801 agcatagtca gctaaatatt cacccccaaa ttcacaggca gcttcaatta aataaataga
    25861 attattgcga atgatacatt ccataagcaa cggtgttgcc tgtaatgcaa atgtatcggt
    25921 tattttttga ccaagttttt gtagctcagg gtacatggta taatattgtg aaggtagaac
    25981 atgcattcta tcaacaaaat atggtttagc attcagtatt ttatcactga tatcataaat
    26041 ataaaatgtt ctgttatgaa caaatcctaa aacaataatt tcatcccctt caatatattc
    26101 ttcaatgagc acagtatcct gcttgactga tttcatatat gaacacaact catggtaact
    26161 atcgataatc ataacgcttt gtttagcatg tccttttggg ggtttaatga tgcaaggaaa
    26221 agatagcgta ttcaatttgt gtaaatcttt tttatgaata ataaaaccat gaggcatggg
    26281 tatgttatgt atagcagcga tagttctgaa gctggatttg tctatgagat catccactgt
    26341 attaaaatca atgtaaggta atttaaattg ttgagataga tatgctacag tttttatggc
    26401 ctggccatat gacctggtta caacagcagc aatgtcatca aatacaagct gttctttcaa
    26461 taataggtat atatcatgat aattatagat ggattctata acttttatat cacaatattg
    26521 gaaaccctct gccagaggat ttttatcaat acctattatg ctatacccca attcttttat
    26581 tgctttaatc agaggtattt gatttttacc tgcaccaatt gatactatga acatgatttt
    26641 ttatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacccaa cagtgctgag
    26761 gctataataa ttgatgtggc agtatcagcc gagaaaccat ttaatagtgc tgatgctaga
    26821 taaattgcta aagataatat gaaccacttt tttataatat ccttcataca ataatcctga
    26881 ttttatcaga aatgccatat agattctgga aacagatcaa ctttttttat ttcatactgt
    26941 tcagccagtt ctgttgtctt ccatgtaagc tgcaattctt ctttatattt ttcataaaac
    27001 ttcattgctg ttttatacct ctttttatga taccattttt cattctgagc agatattgta
    27061 tagtgtaaat ttgatatagt atactgtcta aataaagcat acagaatttc attaatcttt
    27121 tcatttgaat ccttgtattc aacatacatc catatttctt tttgtggatt ttctatgcaa
    27181 tactgatgaa tgggccttaa gtatgtattt tctaattcac cataacacat ccatgaaaat
    27241 gggcgccatg aatgtgatag tacaagcttt ttattaatat aaataatatc tccttcaatc
    27301 atattacagc cataatcaat ggcatcccat aacggacgtt tgcaccagta atcgttatgc
    27361 gaatgtaata atgttggttt ttgtggcaat atatacctcc ataaaagtca agaatatagc
    27421 agttttatac atcattcata cagggttata gatattgaaa atataacatt tttattattt
    27481 ataatttata taatatacca acattaattc ctgtggctgt taaatcattc cctttgtaaa
    27541 ataatctgtt ataattaaaa ccacctgtaa atccaataga ataaaaataa aaaatagcat
    27601 taatttcgac tgataaaaat gggtcatatg aatttttctt aacatagggt gcaaatggcc
    27661 caccttcctg aactaaaaca atatatagat ctgaatatgc aataccaggc tgtatggcga
    27721 ttgcaacatc aatcaatgga gataaagtat ggatatatga taatgagaca cctcccatat
    27781 aaattgaaag gtatgtatca tatgtatgtt ctaaatcacg gttattagta gaaaataatt
    27841 gccatgttgg agcaagatat aatttattta taaatgcact atttgaagta aatatttttg
    27901 aaaatggaac tcctatagag attttactca tcgaggtgtc attataaagt ttttcccaat
    27961 cacctgtaaa gaatatgaaa ttgtatgaat aacaaagaga aatgtttttt aatgatgaat
    28021 aaaaattttt atatggcgat acagcaattt tttccttttc tacttctata gttttttgca
    28081 actctttatt tattttaact gtaattctat caataatttc gtaaattaaa gctccaattt
    28141 tttcatttgt ctcagcagca tcaactatgt ctccggttgc aatgttacaa atctgtgttt
    28201 ttatatgtat tgtatttttt tcaactttat atgagccaat gacagagaaa tttgctgaaa
    28261 cttctttccc tctactggtt aaaatcgcaa tagtattagc tttttcttca tcagtaacct
    28321 gtgaagtaat atatttaaat gttactggta aaactgtaac ttcatattca actttttcat
    28381 taagttctgc ggacagagaa tctggaatta tataagaata atatctgtag ttctgtgtag
    28441 cactttcatc attgaaataa taaatgataa ttttttgttt atcaggagtt tgagcgccaa
    28501 gaaatgctat acaaaaaaaa ttaaaaaaca gcgcaatata tattattttt ataaattgca
    28561 ttccataaat ccataatgaa tatcgttaca atgaaatatc agtaattatc aaataaaagt
    28621 caaggataat tatatgcttt atgttgaata ttcagcattt attttgacat aatcataact
    28681 caggtcagat gttaaaaatt caaattcaga atttccaata ccaaaatcaa cagtgatggt
    28741 atattctcgt ttttttagaa tgatcattaa tttttctttg tcatattcaa ctggagagcc
    28801 atctttaaat aatggtattt catcaaaata aattgacaat ttatcttcca gtagttctgc
    28861 tcctgaatat ccagctgcac aggcaattct cccccaattg gggtcattac caaacattgc
    28921 tgttttaacc agaagtgatt ctgcgataga ttttgcagct ttcttggcat caccatcatt
    28981 tgctgcatgt ataactttta cagtgacaca tttagtagcg ccttctgcgt cacttactat
    29041 aagataagcc aattttttca tcattaggtg tagtgcatga ttaaaggttt ttatatcatc
    29101 cacattatga actgaaggct ttgcagttga aagtatgatg gcagtatcat ttgttgacat
    29161 gtcgccatca atagtaatgg catttaaaga aacattcaca ctttcataaa ataatttttg
    29221 taaaatatgt tgctctacag gaaaatcagt taccacaaaa cacaaaagtg tagccatatt
    29281 gggggctatc atgccagctc ctttggcaat acctgccatt gtatatgtgc cagttgaagt
    29341 tttaaacgaa atagcatatt gttttggaaa agtatcagtt gtcataatag cacgtggcaa
    29401 aagggtaccg gcttcaatac ttagattttt aacaagtaca ggcaggctat ttttcatttt
    29461 atcaacaggt aattgaacac caataattcc tgtggatgcc attaataccg aattttcatc
    29521 aattgaaagt aatttgctta attcacgtgt aagcaatttt gcattttcta agccttgttt
    29581 tccagttgca gcattagcat ttgtagcatt aattaaaata gcattaattg agttattaat
    29641 tctttcacgg cacagaataa caggtgctgc acacatttta ttggtagtaa acacaccagc
    29701 agcccagcaa ggttcactgg aatatattaa tgataaatcg agacgatttg aatagcggat
    29761 accacattca atagctgcat atttgtacaa agaaacgttt tctaaaccac cagctaattc
    29821 ctgtattgta ctcatgtaat tccctcaaat taataggagt gaatatagaa cttttactga
    29881 ttttcaatgg tatgatttac tctttctatc agctctttca attttggttt taaatattca
    29941 aaaccctctt ctggagtatt acaataatgg atcaattcca gatcatgttt atcaatatat
    30001 ccataatcta ccagcaactg aaaattgata aatttatccc agaagctttt atcgtaaaga
    30061 ataattggta tatcatgctt ctggagtttg tttgtctgta ccaaagttaa cgtctcaaat
    30121 aactcatcta aggtgccaaa accgccaggg aatacaacaa tagccttagc atgatataaa
    30181 aaccataact tgcgcatgaa aaaataatgg aactcaaaat tcaattctgg cgaaatatac
    30241 gggtttggca tttgttcaaa cggaagtgat atattcaatc caattgaata tgtacctgct
    30301 tctttagcac ctttattagc agcttccatg atgcccggcc cacctcccgt acagattaca
    30361 aaaccatcat gtacagcttc taattctttt ccgtattgag caagtaaaaa ggctaatctg
    30421 gttgcatcat ggtaatattt gcccgttaca ggatcatcgg gtttagttcg tgcagatcca
    30481 aaaaaaacta ctgtatgctt aatattataa tgtttgaacc gcttttcagg ttcaatatat
    30541 tcacacatta tccgtataat acgtgcatct cgggaattaa ggaaatctag atttttatag
    30601 gctttttcag gacgcattaa tatctccttt tcctgcgcat tgtcaatata gtgccatcgg
    30661 taagtggtga cactgtatcc gaaacaattt tttccttatc tatcagatca atattaattg
    30721 aatccataaa tgcacatata atttttaagc ctgccccaat accttttaac gaaatttgct
    30781 gtccattaac cctgatatgc aatcctgttt ctttttcact ggaatatctt gttatctgtt
    30841 caaaataatc aaacgatgtt ttatctggtg tttttttaat tgattcatta aaattaaaac
    30901 ctttaccatg gtcaataata acagcttcaa attcatcttt tgatacaata tacatgacgg
    30961 ttatttcacg catctcagga tcataactaa tattgagatt atgttctttt cttattgttt
    31021 cctgaatggc attggttaaa gcttcatcca ttgacagtat tatttcttct atttcttctt
    31081 tagggtattg catttcatta agatcagtta aaagcctgtc aataaccatg gggacagtct
    31141 ggctggttgc attatagcta tctatatact ttttaacatc attgttgtta ttactgtagc
    31201 tcatattcac cactaaaatg atagcacact ttaattttat tacaagtatt aaacattaaa
    31261 tataagtatt tggtattgta cttgtttaaa acattataaa acttttatgc aattaaacaa
    31321 ttgaaccaat ttctaattta gtaaaatgtc aactaaattt aaagaaacaa tactttactg
    31381 tatacatcgg ttattcaatg ttttttctaa ttatatttta attttatgtt gaattatatc
    31441 cattatatat attgtatgta taccagtttt ttaattttat tgtaaatatg agcattttac
    31501 caacttcttt ttgaatatat ttcatcaatg atatatattt tcatccttaa ttggttccaa
    31561 cgaatcaaaa tcattcttat tatagtatta taggttgttt tttatttata accgatagtt
    31621 actttttaac agggtaaaga atgtatatga aaatcttttg cacattgctc atatcaatta
    31681 tttcgttcat gggatgcagc tgtggaaaat attattctga agcaaaacag atagttgatg
    31741 atttaaatat ggaatcattg ctaggccaga tgctcattat agctgttcca tcgaataaaa
    31801 tagatgaaaa aactattact attataaaaa attacagacc aggtggagta atactatttg
    31861 gatataattt gaataatggt tcctgtaaat cacttgcaca tgatcttcag aaacttgcta
    31921 tggaatctat tggaatacct ttatttatat caattgacca ggagggtgga agagtaaaaa
    31981 gaattcaaag tggcataaca caattcccgg gtaattttgc actgggtgtt gtcaatgatc
    32041 catcaaaaac atataccatg gcacaaattt taggaataca attaagacta tcagggataa
    32101 atatgaatta tggtcctgtt gttgatgtca ataataatcc agataatcct gtcattaata
    32161 tcagatcctt tggatgcgag ccggctctat gtgcatcatt aggtggagca tatattgaag
    32221 gtttgcaaaa ttcacgatgt attgccgttg ccaaacattt tcctggacat ggggatacct
    32281 atcaggattc acatctgaca ttacctgtta tttacaaatc attgtctcaa ctggagaatg
    32341 ttgaatttgt accatttaaa caggccatcg atgcaggggt tgaatgcata atgacagccc
    32401 atattgcgtt ccccaggttg gggattaata ttcctgcaac catgtcttca ttttttctga
    32461 atgatatttt gcgtaaatca ctaaaattca aaggattaat aattactgat gaccttgaga
    32521 tgaaaggtat ttctggaata caatcatatg gcaatgcagc agtaaatagt atcaaagctg
    32581 gtgcagatat tttgttaata agctcctatg acaatcatgt tcaggagatt attgattcgt
    32641 tgaaaaacgc tttagctaat ggtgatattt cagtagaatt aattaaatca aaagcaacaa
    32701 aaattctgga attgaagctc cggtataaca taatgcaaat caaagatggc aaacttttat
    32761 taaaaaatta tgagcctgaa cctgatgatc taaaactctt acagcaagca gatgctctta
    32821 atacagaact atcccagagt tctattgtgt tttattcaaa aaacacgata catgattttg
    32881 taatcgataa tcgcaatacg tatgctgaag ttacttcaaa aaatgacaat cttattagta
    32941 tttttaaaaa agaaaatgta ataatggatt ttccggcaag taataaaaag aacaaaatac
    33001 tattgattga tgcttcatca tattcaataa ccgaattaca tattttgctg aaatcattgc
    33061 aatcttataa caaagtattg ctacttatta ctggcaatcc atacccttac atcaaacact
    33121 ttggaaacta ttccatgctc atgtcatttt ccgatactga tgaatcatta cgtcaactgg
    33181 cattgtgttt gaaaggtgta tttgaaccaa aaatttcaca tccctgttta cctggtaata
    33241 ctcaatgaat ttaggaattt acatccatat tccattttgc cataaagaaa aatgcgatta
    33301 ttgtgatttc tattcaatac cggtaaaaaa gaataaaagc tggcatacat taattgaaaa
    33361 gtatataaaa acactttgtg cggaaataat atattattca actgaatttc aagatcatgt
    33421 cgttgatact atttactttg ggggtggtac cccatcatta cttgaaagaa atcattatgc
    33481 aaccattgta aatacaataa aaaattatta cgcgatagct cttgatattg aaatcactct
    33541 tgaatgcaac cctgatcatt attcattgaa ttatattaaa gaattacaat cagttggaat
    33601 taaccgtttt gtactgggag tccagaccct tgacaaaaga gcacatgaat atattggtag
    33661 aaaaccaaaa cctgccggta gggaaataat agaagaattt tgcagtattc ctgatattat
    33721 ttcatgtatt gatatcattg ttggtattcc caaacaaact gttacaagct acatttattc
    33781 actgaacaca ataatacagt gtaaacccaa acatatttct gcatatatat taacctttga
    33841 aaaaaatact gtcttaagct cacgagttcc acactcagaa aagttttcaa actttcagcg
    33901 tacaatgttt gaaaaaacaa taacaattct taccacttca ggttatcgtc attatgaagt
    33961 ttcaaatttt gcactaccac aatttgaatc gcgccataat tgtaaatact ggaattttga
    34021 agaatatata ggctttggtg caggtgcgca ttctttttac aaacaaagac gatattataa
    34081 cagcaatctt aacgattatc tcaataatcc tattgacagc cgtatagaag atgtccggac
    34141 aataaaggat attgctatag aataccttat gaataaatta cgtctgatag acgggttttt
    34201 tctcaatgaa ttgtatataa aaaccaatta tgttccatcc tcaggattta aagatgctat
    34261 tgaaatatta attgaaaaaa aattattaca acgttctatc atagaagggc aggataaatt
    34321 gcagtgtact tatgaaggta tgtttatgtt agattcagtt ttatttgaat tgatgaatag
    34381 tatttaatct ttaactacaa aatatttgct gtaaatggtg tttatccttg cataataatc
    34441 tgcaaggcgt tcattgttaa tcaacgtaaa gtaatatgca aggaattgag agccacgtac
    34501 aacataatta tttaaatcac gccctcgtaa tcctttgata atttcaacat tccctttttc
    34561 aatatatgca agtcccaatg caatatagcg ttgcaaattg gatatatctc tcttcagttc
    34621 atcatcatta ctggccacta actgtgaaac tttaatctca tccaaaccac ctgataataa
    34681 gaaatatttc tttatatctt tcattgaagt ggcaatagtg gctctgttaa cagcatcttt
    34741 gaataatgca gagaattttt caaaggcaac cagtttttcc tggtcagttt ctggttcatt
    34801 accaaatatt tttttataaa gattaaatgc aatcttttct acttcctgac aatcaggatc
    34861 tgcatttatt actgctttta atttatgata attctttggg tttctgtcca atattttgat
    34921 atagagatta acaccattca gtgcttcaag atatattggc aaattaccag atgaaaaatc
    34981 cctttcagta tattttttca ttccttcaat aatcatcgat gattgggcag taataagaag
    35041 cgcatccata tcaggctgga aatctctgga tgagcgttta cgtatactat ctgaactatc
    35101 tttttcaggt ttaaaatctt ctgtatcgtc atctactttt ctgttttttg cagtaccatg
    35161 ttgctttttt tcttcaattt catcatccaa tttaaacaaa tcatcatcaa aagaaaactc
    35221 atcaatctct gacattacag caaacctcat taaaattatt aaatcaatta tatctattta
    35281 caaagataac cggtatatat cattttttca agctctttta taattaatta ttcttaacat
    35341 catcatgtcc gtacatttct attcttaatt ttcgtaattc attattcatg taattccatg
    35401 tatactgtgc cagctgttca acagtcatat ctttatatgt ttcaggtgtt atctcatcaa
    35461 gtacccttaa agtaatttta tgtttgccat gataatcaat gctgtatttt ggcaatgcaa
    35521 agcgtgttcc ttcaataata acaggcatga taggaacatt catgttcttt gccagtataa
    35581 aagcacccat tttaaactcc ttaagatgac catcaggtga tcgtgttcct tcagggaata
    35641 taaatacagg gctaccttct tttaaatgca tttcacaatc ctgtaacatc tgtacactac
    35701 tttccttaac acctctaaat aaccttacat atctgttcag gaacatattc cagccaatta
    35761 ctggtacctt aaaaatttct gcctttgaaa cccatttaaa atgtttatat agcctgaatg
    35821 caaccagtat atccagctgc gactgatggt ttgatacaaa tacatatggt acattatttc
    35881 gtatcttatt ccttccttcc acttttatct tccacggagg catcacatag gtatataatg
    35941 aaccccagaa acatgtgagt aaatgtaaca accgtaacct tttatcaaat gggtaggtga
    36001 aaattcttat taataatgca actacaaaga aaatcaatga agtaacagct acacatgtta
    36061 gaaaaagcgc tgtaattaat ttacctttca tactggttaa actccgttgc aaataggtta
    36121 ttataatgtt atctgtatgt tgtttacagg gtaatattga ctgctatatg tagcaaactc
    36181 tgttgaaaac aaaatatact tcttatcata gtaaaaatca acaaaatatt ttatttttat
    36241 agaattttaa tatgtcatta gtactgctca aaactacttt atagtatata ataattgaat
    36301 ttaataccac cactaacggc agcaaagata ataaagcatt ttttgcctta taaaagttaa
    36361 cgaataaata ttaatattat tttatgcatt attttgtaca aaatcgatga attgaggaat
    36421 aaattcaaat atatcagcca ctactccata atgcgaaaca ttaaaaattg gtgcgtatgg
    36481 atctttattt attgctatta ttgtttttgc ccctttaata ccagccacat gctgcgaaga
    36541 acctgaaatt gcacaggcaa tatatacttt gggtgcaatg cttttccctg ttatgccaat
    36601 ctgcttgcta tatggtagcc atccataatc acatacaata cgtgatcctg ctacagcact
    36661 gtgtggaaaa acttttgcaa attcatatat caaatttaaa ttttcccttg aaccaattcc
    36721 tttgcctgca ccaacaatta catcagcctc atccagtgat gtatccttca tatcctttgt
    36781 aataaaatta caatatatat gtttattgac ttcagggatg tgaatcgtcg ttatgtgttg
    36841 tgtttgcaca tcagtaatgg tatatggctc aaagctgcca ggaagtactg taagtattat
    36901 tggagtatca tttataacaa tatggcgatc taacttgcca gcgtggctac cacgtgttac
    36961 tgtaatacgt tcagcaacat ctaaagaatg tatttgagta atacactgtg cattaatact
    37021 aactgatagt gctgggatat agtctatcga aaatggtgta tgcatcccaa taatatacga
    37081 ggcattaact tctctgataa ctgaagaaat atacttaaca agaacatgtg cactgtactg
    37141 ctcaatgttt aaatctaata taaaggtttt aaactcactt gaacagtaac tatcgccacc
    37201 aatatttcca aagggaatca atatatatat aggcatagta ttgtttttag caattttctt
    37261 tgcagcagat agcatttcta atgttgaagg atttattgta cggcttttaa catcagcaat
    37321 aattaaaata ctcatagtgc ctcacaaaac tgcattttta tggagaatag tataaagctc
    37381 agtaaccttt tgtgaaatgc ttccctcaat aaactggcat tgcgtggttt tttgtggcat
    37441 ggtaaaatag cttttagttt tcccagtgga cgttattaaa tcttgtttga taattgcaat
    37501 ttcctgttta cgtgctcgca gtacgtttga cagggaaggg tagcgtggtt tatgtatact
    37561 tgattgtacc gaaatgacac acggtatgtg taattcatac atcagtgatt cctgtgcatc
    37621 caactcacgg gttatggtta tggtagtatt attaagcgat agttccgtta caccagtggc
    37681 atgtttatat cctaacattg ctgcaatcag ctgtcctgtt tgctgattca ttgtatcttc
    37741 ggacattaca ccagtgaata taatgtcata gttttgttgt gcaaattgtg ttattgcctg
    37801 tgcaattacg tatggattat gtaatctatc atcgtcggat tgtatgtgaa aagcttcatc
    37861 aactcccatt tccagtgaac gtcgtaatgc tttttgtaat cgatggggtc caacagaaac
    37921 agcatgtatg tacgatgtta aaccacaatc ttttaactgc aatgcttctt caagggcata
    37981 ttcatcatac cgattaatca catatactgt tttttcgtca tattcaggta taccatcatt
    38041 taaatacact aatgcattgg tatcgggaac ctgtttaatg cacacaagga ttttaaattt
    38101 attttccata atttgtacct gatagtatat gttgcgatta caatggatag ctataataat
    38161 ttttactgta aatccatcat attataatag ataaaaaaaa tcaacaaaat aacgaacgtt
    38221 cgttatattt ttcttgactt tttctacagt gaatttacag tgtatattat atgataattt
    38281 taattaataa attcatacac aattaacagc tcatcacaat ctttacatgt ggcaggcgtg
    38341 ttgtatttat tagcaggact atagtatgag tgaaatattg gttatacggc atggccaggg
    38401 ttcttttggt cagggtgatt atgataacct ttcagaaaaa ggctataatc aatcccagat
    38461 tctagctgaa tatttataca actatcgcta taagaataaa aaaattgatg cactatattg
    38521 tggtacacta aaacgacaac aacagaccgc ccaaactatc attaagtatt ttgaattgaa
    38581 gggcgttcag ttacctgaac caaaaattga taggcggtta aatgaatatg attcaataac
    38641 aataataaaa acattatttc ctgttttgct tgatgatgaa ccttcacttg aagaggagat
    38701 caagcagata tatacagata aaaaagcttt tcagcgcgta tttgaaaaag ttatgcgtta
    38761 ctgggtaaaa tcacagacaa attatgaagg aattgaatca ttttcagaag tcaagcaacg
    38821 ggtaaaatct gctattactg aaattataca ggaaaacgga agaggaaaaa atattattgt
    38881 tgtttcatca ggtggaatta ttggtgctat tgtccagatg gttcttggtt ttgatgatga
    38941 aactgcaatg cgccttacat ggcagatagt taacacctca ttgactcatc tcagatataa
    39001 cgaaaaagaa ctcattttag catcgtttaa ttttttccca catattgagt tagccgatga
    39061 tccatctctt aagacgtata gatgatgtaa cattttaagg agggtattat ggattttgaa
    39121 atcagtgata agatgaaagc cattctggaa cttattgatg aatttgtgga aaaagaactc
    39181 atcccgcttg aacaggaatt tgctcgtaat gatatcaagg caatgctgcc agttcttgaa
    39241 gaaaaacgca aaatggtaaa aaagatggag ttatgggcac cacaggttcc aaaggaatat
    39301 ggtgggatgg gccttgattt agtagaacac ggtcttgtct ctgagtcatt aggaaggact
    39361 ccattgggcc actatgtatt tggctgtcag gcacctgatg caggcaatat tgaaatactg
    39421 attaaatatg caaatgatga acagcgtgaa aagtatttgc tgccacttgt tcgtggggaa
    39481 atacgtagtt gcttttccat gaccgaggtt gacctgcctg gatctaatcc agtattgctt
    39541 gatacaaccg cagttaaaga tggtgattac tacgtaatta acgggcataa atggtattca
    39601 actgccgcag acggctcaaa attttcaatt gttatggctg tcaccaatcc tgaagcacca
    39661 acataccttc gtgcaagcat gattattgta cctactgata ctcctggctt taatctggtg
    39721 cgcaatattc cggttatggg tcacgaaggt agtgattatt tcagtcatgg tgaaatatta
    39781 ttacaaaact gccgtgtacc ggtaacaaat ttattgggcc ctgaagggca gggttttgtg
    39841 atagctcagg aaagattggg tccagggcgt attcatcatt gtatgcgctg gcttggaatc
    39901 tgtaaacgcg catttgattt aatgtgtgcc cgtgccaaca agcggattat ttcccctgat
    39961 ggcaaaacac ttgcaacccg ccagattatt caggaatggg ttgccgaatg tgcagctgag
    40021 attcaggcag cacgcctcat gactttacag gcagcgtgga aaatagagca cttgggtcaa
    40081 aaagaagcac gcattgatgt gtcgcttatc aaattttatg tggcaaatgt tatgcaaaag
    40141 gttatagaca gagcattgca ggttcatggt ggtctgggaa tgactgatga tactatattg
    40201 gccttctttt accgacacga acgcgctgca cgtatttatg atggtgctga tgaggtgcat
    40261 aagtcagttg ttgctaaaag gatcttgtca agctatgaag gcagagaagt cagatagtat
    40321 tcatattgtt gacctggaaa actttaacag ttttaaaaaa agtataaagt ttatccttga
    40381 tgatattgta agtaagttat atgatgataa taagctgctt atcagggtta agaaatctcc
    40441 acaatccatt gaaaatctta aacggattat tcatacggca atagcaataa gcaataaaaa
    40501 aggctatgct gtaatgagca tacgcgaact gtcagcagta tcatcgttaa gtattggtgc
    40561 attatatgca tatttccctt caaaagatga attactgcat atcattcaac agtatggccg
    40621 ctcatctgta gaaaaggtat tatccgaaac actggtgcat acatcatctt caaaagataa
    40681 attatataat tttatccgtg ctcacatctt tatcagtgaa gttttaaaag actggtttta
    40741 tttctcatac atggaaacac gatatttcac tggctctgaa tataataaaa caatagaagg
    40801 tgagctgtta acagaatctc tgataaaaga tattattgaa aatggtataa aaaataatga
    40861 attccgttca gatatacagg cgtcattgat tgcctcaata agcaaggcca tgttgcagga
    40921 ctggtacctg aaacgctgga aatacactaa aagaggagta acgcctgatg aatatgcact
    40981 tgaatgtttt aagatattat acaactatat aaaactttga aaactggagg aatcttttat
    41041 ggataattat attgataagc caactgacat cagggaaggt gaaacgatag atgggaaaat
    41101 agtactggat tatcttatac agcatgtccc tgatattaaa gggcctattg agataaaaca
    41161 atttcctagt ggattttcaa accttacata ttttatcaaa gccggggaca tagaaatggt
    41221 gttaagaagg cccccttttg gcaaaaaggc aaaaactgct catgatatgc atcgtgaata
    41281 ctcaatactg aaagcattaa aacctgtatt tccatattgc ccaacacctc ttgtatactg
    41341 tgatgatgaa tcagtcattg gcgctaaatt ctatgttatg gaacgcatta aagggattat
    41401 attacgtaaa gaactcccgc caggattatc atatacaccc gaagaattcc gatacctttg
    41461 tgagcagtta ctggatatac agtgccagct tcattcactg aattataaag aaattggact
    41521 ggaaaatttt ggcaaacccg aaggatatat tgaacgtcag gttaatggat ggattgcacg
    41581 ctataaagat gctctgactc ctgatgctcc actgtacact gaagtcattc agtggttaca
    41641 ggaaaaaatg ccaaaaacac caacacgggc tgcggtgatt cataatgact ataaatttga
    41701 taatgttgtt ttggatatta atgacccaaa aaggataatt ggtgttcttg attgggaaat
    41761 ggcaaccatt ggtgacccgg ttatggattt gggtaattca ttagcttact gggttgaaaa
    41821 aaatgatccg gaagaagtac atcttatacg tttaatgcct acccatcttg atggagcact
    41881 tacgcgaaaa gaactggtgg agcgctatca agaaaaaagt ggtattgata tgagtgattt
    41941 tgattacttt ttctgctttg gattattccg acttgcagta atagctcagc agatttatta
    42001 tagattttat catggtcaaa ccaaagataa acgatttgct atgttaattg ttgctgtaca
    42061 ggttttagaa cgacaggcaa ggaaggttat agataactca aaactttaat ttacatcctt
    42121 ttctaaaatc tctttagtaa gattcatgta atctacggca ccattactgt tgggcttgta
    42181 ttcaaatata gtttttcccc agcttggtgc ttcttgaagc aatacatttt cacgtattag
    42241 cgtttcaaac aatttacctg gaagtcgctt tttagtttct tcaattacct caacgtttat
    42301 ttgttttttt ggattaaaca tggtaccaat aatgccggtt atttcaacct ctttgttgag
    42361 tctacgtttt accatttgta ccgcttcaaa cagattatac ataccatgga atggcaaaaa
    42421 ctgaacctgg agaggaacca gtatctcatg agcaaatgtc agagcattca gtgtcagtat
    42481 ccccaatgaa ggagggcaat ctatcaggat aaaatcataa cctgagatac cttccaatgc
    42541 ttcttttaat aaaaattctt tggcaggcaa tggaataaat tcaatatctt tcagttcatt
    42601 ttttgatggg agaatatgta atccatccct atcaattatt acatctttat atgcaatttc
    42661 ccctttaagc acattatata ctgaatatgt taaattattg gggttatacc caaaatggta
    42721 tgttgactga gcctgatggt caatatcaat aacaagaact tttttcccta aaatctgtaa
    42781 ggcagcggca atgttaacgg tactggtagt tttgccaacg cctcctttgt tgtttgaaat
    42841 agcaataatt ctcactggaa taatcctgaa ataatgttta tgttacagtt actatcgcac
    42901 acgttaaaac atatttttaa taaaattttt cttattgtat ctataaaagt attgcatatt
    42961 aatatactat tcatttgtgc ttccatatat gatacataac aataaaatac aagtaaaaaa
    43021 atcaaaatta cgccaaacac tcaaagcatc attccttgac ggaatatttg caagcataat
    43081 gtttggttgc gttcaggatt actttacccc gtttttgctt ttgatcaaag gcactgttgt
    43141 gcacgtggga atattaagtg caattcccaa tataacagtt tggctccatt ggtaaccctg
    43201 catgggcaag cctgatgagc gatctcatcc ccgcgcacaa aaggggaaaa tactttggat
    43261 tcagaaatag cattcttgga gttttacttg ttctggtgtc attatcagtt ggaatcacac
    43321 tgcggttttc tcccgcagag tatatcatgc aaacattttt tgtagtattc cttatagctt
    43381 ccatcagtag gtttatctca ttctactatc ttttgaaaat gcacgaaccg ccatttgaaa
    43441 ttgaaaaggc aagcatagtc ccagcaaata tatttaacag cattaaaaaa aatgattttt
    43501 taatttttat agcttttatg tcattaatga attttgctgt atatctatca gcaccatttt
    43561 ttgcagttct catgctacaa catctttcat tttcctactt tgagtattca ttagttacgg
    43621 ttagtgctcc ttttattatg tatattgcca tgcactcgtg gggcaacata gctgataaaa
    43681 ttggtaatgc aaaagtatta actatcacat caccattaat agcgatagtc cccatattat
    43741 gggttttgaa ttctcatatt gtttacttgc ttattgttca gatattttcc gggcttgtat
    43801 ggtccgggtt tactattagt tcattcaatt atatgtttga tgcatcagta agttccaaac
    43861 gtgttagata tatctcatat ttaaatctca ttaatggcat aatgattagc gctggagccg
    43921 tatcaggtgg ttatttaata cagatattgc ccccagttaa cggaagtagt atttatacaa
    43981 tgttacttgt atcgggaatt cttcgattga gtataaccat tgtttttggc aaacgattaa
    44041 aggaaatgag attggttaaa atataatata agctgttttc cttatttaaa taaaaaaatt
    44101 aaataaattc taataaaatg ttgacaactt agcgaaatat tagtatatat gttacaaaca
    44161 gtaaggttgt aagtataagt tttatttttc gtaaaaattc cttccggagt tttaaaaata
    44221 aaactaattc acatcttact gataaggctt ttagccgtat tttatttatt taaggagttt
    44281 ttcacatgcc taaaggtaca atcaagtggt tcaatgaaaa aaaaggtttt ggattcattt
    44341 ccaaagaaga cggtagcgat gtatttgtac attataccgg tattattggt aacggtttca
    44401 ggacattaaa agaaggacaa aaggttgaat ttgagatcga aagcagcgat aaaggtccgc
    44461 gagctgtttc cgttagagcg atataataca attgtaaaac cacttatcaa aagccgccat
    44521 cgttggcggc ttttttattt gttatatagg attttttaaa gaattttctg tgtgaattag
    44581 aaatctttca aacaattcag tatggtcaat accaaaggct tctttaaagt atgtctgacg
    44641 catcataatt atctgtgtaa ttccaaaaac ctggccccat agtattacca taagctgtct
    44701 tggtgtcaga gttgtatgta tgctcccttc ctgtatgcca tattctatag ctttcaatat
    44761 caaatcagta ttgttatcgg caattttttg gcactcattt ttataaaatc ctataccgtt
    44821 tgaaacttgt gctggatagt atatacaatt tctagcttca tacgtcatta taatgttaaa
    44881 ataatgagga tattcaagaa agaatgtaaa aaaagccttt cccatactgt aaagcttagt
    44941 atgtccttca actgggttat catatgcttc ctgtaatttt ttattgaata atttaagtgc
    45001 atctaaaacc acagccaaga ataattcttc tttatcctta aaataatgat aaagtgttcg
    45061 tttattataa cctgcacgtt gtgctacttc gtcgatagtt gttgtatcat atcctttctg
    45121 aaaaaatatt ccctgtgcaa tttgaataaa tctgtttact ttttgttctt tctcccattg
    45181 cattctttca gcttttttca tagtcttgca agttcccgaa tttaattatc aatatataac
    45241 aaataaagat tagtaataat aaagtatcat attcttaaca taggtattat aatattttca
    45301 ttaaatatac gaatgctttc aaggaaattc ttatgtaata gtaaagtcaa ataaaaaatt
    45361 ttccttgaca tatttcacta tagtgaatta tttttcatta tagtgaaatt tatggggggg
    45421 ggtctatgga ttctttacat tcagtctttt taatcagctt ttttattctt actccggcta
    45481 taataatata ttgctgctac agattccctt tgataaataa aattggacct gtattattgt
    45541 gttatattgc tgggataaca ataggtaata taggagttct tcctgataat gcaaaacaaa
    45601 ttcaggatat tggatccaat atttctattg tattgggtat tcctctactt ctttttacta
    45661 tcaatgttaa aacctggttt aagttagcag gtaaagcgtt gctatcattt accctgggaa
    45721 gcgttgcggt tgttattatt gtctttattg gatacatatc atttaaacat tccatacccg
    45781 atgcatggaa gattgccggg atgcttattg gcgtttatac cggtggtact cctaatttgg
    45841 ctgctattaa ggaggcgtta caggttgatt caaccacatt tgttattgtg catacatatg
    45901 atactgtgtt ctcaatgctc tatataatct ttttggtatc aatagctcag aggatttttt
    45961 taaaatttct tccagctttt tcagttgtaa atgatgaaga aaaaaatgca aaaactgatg
    46021 aagacataaa actttatggt gatattttta ccaggaaaat atttcctcaa cttatcattg
    46081 cattattact gtcaactgtg gttgtagcta tctcatttgg aatttcactc gttgttcctc
    46141 aacaatatca aacaatgatt gttattcttg ctttaaccac agttgcaata cttttttcgc
    46201 tcagtcctta tgttcataat cttaaaagaa cattccagtt tgggatgtat ttaatttata
    46261 ttttttgcac aatagttggt tcaatggtta atgttgacaa tcttattcat attaacatgt
    46321 acctcctgtt ttatgtgttc atcgcaattt ttggaagttt acttttgcat ggcatttttt
    46381 gcaaaatatt ctcaatcgat actgacacct acatcattac ttcagtagct ggtatatgtt
    46441 caccaccatt tgtacctgta gttgctgatg cgcttcacaa taaatatatt attttatcag
    46501 gaattactac tggtgttatt ggttatgcaa taggcaatta tctgggtatt acgttggggt
    46561 atttattgag tagcatgtaa gttttcaacg gcgattactt tatattgtaa tattatttaa
    46621 tagtatagga ggaattaatg aatttcgatc aactacctca caaaggatta tcatatacta
    46681 aagtactatc tcttatgaag cagtatggag ttaatgatgc cagctggaaa aaaggaaaaa
    46741 cattcagcct tgtctatcac agggatgata cacatacaaa atttttacaa aaagcatttg
    46801 aactgtattt tgatgaaaat ggcctcaacc caatggcatt taaaagcctt aaacagtttg
    46861 aacatgaagt agttaaaatg acagcacata tgctccatgc tgggaatgaa gcctgtggta
    46921 ctatgacttc cggaggtact gaaagctgcc ttttagctgt taaaacatac cgtgatatgg
    46981 cacgaactca acgaccatgg ataaagaagc cggaaatggt ggtacccgaa agcattcatg
    47041 ttgcatttga aaaagctgct gaatattttg gcgtgaaaat tgttcacgca ccattggata
    47101 aagattttaa agtagatgtt aaagccgttg aaaaactcat caaccgtaat actattctta
    47161 ttgttggctc agcgccctgt tatccgtatg gtgtcattga tccaattgat ttattaagcc
    47221 agatagcaat taagaaaaag ttacctcttc acgttgatgc gtgcttaggt ggatttctat
    47281 taccatttgt tgaaaagttg ggctataaat tgccaccgtt tgatttccgt gttgaaggag
    47341 taacatcaat gtctgctgat gtccataagt atggtttcag tgcaaaaggt gcttcggtca
    47401 tagtatacag aaatattaat tatttacgtc atcaattctt catctatgaa gattggtgtg
    47461 gcggagtttt tgcctcacca gccttgcttg gaacacgacc aggtggaaca atagcagcag
    47521 catgggcagc aatggttgct cagggtgaac aagggtatat gcataatgca aacattatta
    47581 tggaaactac acaaaaaatt caaaagggta tttcatccat taaagggatg gaaatattag
    47641 gcacacctca tatgagtgtt tttgcatacc gttcggttga tcctgaagtc aatacgtttg
    47701 ccattggtga tatattagaa aaaaagggat ggcatgttga tcgattacag cgccctgatg
    47761 ccctgcatgc aatgattaca ccagtacatg ctaaagtagc tcaccagttc attaatgatg
    47821 tgaaaaatgc atttgagcat gttaaaaaga acccccatct ggcgtacgaa ggaagcgcag
    47881 caatgtatgg tatgatagca catataccca tgcgcaaaat ggtaaggaat aatatattaa
    47941 aaatgatgga aggcatgtat ggtcctacag gccagattcc tgatttaagc atggaagaag
    48001 atactaaaga agactttgcg atgaaagcag gcaaatggta tcttaaacta agagataaat
    48061 taattaagta aaataatcag tacattaaat gcactttttt tgtttatgcc actcgtacaa
    48121 tgctatagat actgctacgg tggcattcag tgattctact gtatgaattg gtatctttaa
    48181 ttttactgct ttatctatta caggcaaccc gggtccttca gttcctgcca ccagaatagc
    48241 ttttggtggg aatttgtaat cgtatatatg ttctccttcc atatcaagtg caagaatagg
    48301 gcaggatatt gtcctggtat taagcatatt caatgctggc cctttttcaa tggtacaatg
    48361 aaacactgct ccagcagatg cccttactgc cttttgatga aaaggatagg cagcattttc
    48421 acacacaata atatgtttaa caccaaacgc aacagcactg cgtatggcag cacccatatt
    48481 aacgggatcc tgaaagggaa gcatgagcga tagttcctgg caggtgtaat cccactgagt
    48541 aagggctggc acctgtacag ccatcactgg ctgatctgct tccgtaatat ctatctcatt
    48601 aaacagcgat tttttcagga taataattct ttctttgcca aagtttgaaa ttagatgtaa
    48661 aaattccatg tctgattcta catactcatc atatataatt aaatatttgc acaactcagg
    48721 ttttgaattt attaattcct taattatttt tttcccggaa acaaacgtgg tattgtactt
    48781 ttttgggtta agtgcaatct ttttaaattc tttgaatcgc ttgttttcag atgacgttac
    48841 tgcaatgcca tcggttgatg aaccatcctt agcaatataa taaattttct ttatttcatt
    48901 ggttgtcttt ttataaacaa caagccttct ttgatatgtt gtatcaggca acgtataatg
    48961 tttatcaagt accaaactat aacttccatg ttcatctatt ttttcttcat caacggcagg
    49021 tcctttcata aatataataa gattatcctt gattaaaaaa tgtctgcatc gatgcaatgt
    49081 ttctgcaatg gattcaacag cacgagtgat tacaccatgc acgttaaaaa atgatttttc
    49141 ggtaactaag tgagggtata tttctacatt atcaaggtgc aattcatttt ttacgataga
    49201 aagaaattca attcttttgt gtctgctttc tgctaatatg aaagatttat ttctatgaac
    49261 tatcgccaaa ggaattccgg gaaaaccagc accggtgcca atatcaagaa gtggtgaagg
    49321 tacatcaatg aatgaagtga tatataatga atctacaaaa tgttttacaa taatttcgtc
    49381 tatgtctttt atgcgcgtaa ggtcatgtga atcgttatat tgtaagagca gtttcagata
    49441 cagggataat tgttgtacag cttcattact aaattggaga gaggattttt ttaaatagtt
    49501 actaattttt tgttcataca tgatgctaca tttaccatag caccttcatg gggtttatca
    49561 gtttattata ttgccatagc gtaaaatgta aatgaggccc ggttgaccat ccagtggaac
    49621 caactcttcc tataaactgt cctgccttaa ctttttgccc gggacgaacg ttaattgtag
    49681 acaggtgacc atacagagtt ttaaagcctt catcatgctg tatgattact gctttgccat
    49741 atccacccat ccatcctgat gaaataacaa cgccgccacg tgcagcacca actggggtgc
    49801 catacggagc tgctatatca agaccattgt gaaatcccca ttgcctgaaa atgggatgga
    49861 ctcttccacc aaaaaatgaa gtaagtcggc cacctaaagg ggaacgaaac atttctcgga
    49921 gtgcaaattg ttttgcaagc tgcggagtca tcacatcagt tattggccgt gcatccttta
    49981 tatatactgc aacaggttta tccatttttg ataccaggga ttttattctg taaagtaaaa
    50041 gagacggtgt aaggacatta ttttctggaa cattgtataa atcgtgtaat tcactgattt
    50101 cactaatatt atttacaaaa tgcaaaactc ctttctgaga aggtataact actttctggt
    50161 caatctttgc aacaatgctt tcccataatg ggttggcacc aatcagagta tctatatcaa
    50221 tattatactc tttagctatc ttccagaaat tatctccctt ttcaatttga caaacagtga
    50281 aggtaacacc aggtatgtta agtgattgaa tgtaagtaat atattcattt tcatttgcaa
    50341 agatatctgc aggttttaat atggtagtat cagaaatctg tgtattaaaa atttttacta
    50401 tatataatga aatactgaga aaagatataa tacttactgt taaaataatg aaaattattt
    50461 tatttttatt catcagttac ctgcccgggc aaattgcgtt tattgttctt taaaatacat
    50521 aaaatactca aataacaagc acaatacacc tattatttat tacgagtata ttatcgatat
    50581 ttattgtcaa aaaaattatg ttaattgaat ttagtctgca tggattgttt ttgcagtcaa
    50641 gaactttttt tctacaatga catgttttat aaatgtagta tactatatga gccagcgacc
    50701 aggatcgaac tggtgacctg cgcattacga gtgcgccgct ctgccaactg agctacgctg
    50761 gctttgtgtg tataatttat agcaataata cattgtcaaa tcaaaaatta taaatttaaa
    50821 tttatagtaa agaaattaaa ttcaatatat taatggtcaa taataaatgt aatgattgca
    50881 tagttaattt cagtccaaat agattatttt ataaaatatt cttgattatt tatgccgatt
    50941 tattataagt aaacaaaatt taaataaaaa aatacatttt taggggtttg gcattatggg
    51001 gtcaattatt ttgtttaatt ccttttcttt agggacatta attcctttta tatttttcat
    51061 gaccacagga ttatttttgc tcaacacaaa aaataaatcc agagcaactg aagttatggg
    51121 aaaagcatat gttcttatgg gtctttttaa tgtgggttat cttatttctt caagtatata
    51181 tcacccttat gctgcatttc acagatggtg gacagtcttt tttatcttaa tagcaattac
    51241 atattttggt tatttctatt tcttctttcc cactacgaaa gcaccaaggg cagcaaaagt
    51301 atatcttatt actgggacca tcattacctt aatctcactt tctgcattta ttgttgtatc
    51361 tttacaatca aagatagtat atcaattcag gggccattat tatgattttg atgcagagga
    51421 tatcagcaag tatataggca ttattattat gtttggaatc ctgtgttcat atattgttgc
    51481 aggatggaaa atagctattt cttcaaaagc cgaaaggaaa gtaatactat tgctaatatt
    51541 cttttccatg gttggcacta tagttccctc tattacaaat acattgagca gagcaggaac
    51601 tattgacagg gaagtttttc acacaacctg gacgcttttt aatcttttag gctttttctt
    51661 aaattttata gcatacatta ataactctaa agaccgcatt tcatttctag gtaaactgat
    51721 aggaataatt ctggttactg ttttatgttt attgcagata gttggattca ttgtactgca
    51781 ggataaagaa aataattttg acaaaatata ttatcaggca acattgctgg cagtatctaa
    51841 tgaatcacca caagcagagc atttatatat atcaaaatac aatgttaacg ataacaaatg
    51901 gttaaaaaaa cttcctgaaa atctagatga aaaagaaatt actaatgaat tgatcaacac
    51961 atatatctgg aatcagattt tcacagcttc tgaagataac ctttctcaat caattatttc
    52021 tattattacc agcaatacta ataaattttt tagaggatat gctctttcac ttcaaaacta
    52081 ctgtacacat aatgcaaatg ttttaaacaa acaaacattg gttgactatt gcaaaatgct
    52141 tgataaaaaa gtttatcatc ttaatcatca gatttcttca ttaaatacaa aaaatttcag
    52201 gtcttattta aaacaaattc tttccaaacc tgataaagca atcacaggct ttgtaactgc
    52261 aattaatgaa tttattgacc aatccccaga agagggtgaa cagttaaagc aagcggtcaa
    52321 gcaatttttg ttaccagttc aattccctca acaaaggaca taccatacaa cattggataa
    52381 taaatcacat attatcgcat atcgtataat tgaagatgat accgtgactg aagtaggttt
    52441 tccatatgag ctataccgca aacacatgaa ttcaacggtt ataacattga taatcattgt
    52501 aatttctgct tatatcttta tacgttttgc catacgctta ttttttgccg ggattcttat
    52561 aaagccaatt agaattctca ctgaagggtt gcgtgaagta gataagggca atttcaatat
    52621 acatctgcca gtgcaatcag gggatgaatt tgggtttata accggtgaat ttaataaaat
    52681 ggttgaatca cttcgcaatc tatttcaatc aacgcaatca aaatctgaag aagttaaacg
    52741 gctaagctca gacctcaatg tatccgccac taaattatac gatattgctc gtgaactttc
    52801 taccatagtg gaagagacat ccagtgctta tgaggaaatg tcttcttcat ttgaatcaaa
    52861 ccttagtgct ataaaacagc aagctgatag catggatgtg attaaaaatg atattgaagg
    52921 tatagatgta agcagcaatc agatatcaca aaggataaca cggttatccc attcaattga
    52981 acaggcagtg aaacaatccg aagatggaga agtaactatc acaaagtcaa tcaatgctat
    53041 tgaatcaatt gctgaatatt taaaagaagt tgaacagacg atagtcaata ttaatgagat
    53101 agctgataag attaatttac tggcattgaa tgctgcaatt gaagctgcac gagctggtga
    53161 atctggtaaa ggattttctg ttgttgcaga tgaagttaat aaattagctg accagacggc
    53221 tgatattgtt aaaggaatac actcaacaat ttctgaacaa gccaggatga taacaaatga
    53281 aattcaatac ataagcaagg caactgaagc tatcaaagct atacgaaaag gtattacaga
    53341 aacatttaat gttcttaaag atacagttga ctttactaac gagttaaata ttaaaaataa
    53401 ggatattaaa acaaaattgg aatcttttaa agaattatca ggctcagtat ataatttttc
    53461 catggaacag aaagttactc ttgatgaatt aacgaaagct gtaaatgcaa tcatagatat
    53521 aagccagaaa gcactggaaa gtgcagagtt tgttcaggga ttttcacgta ttctcgattt
    53581 aagtgctcag gagttaagtg aaagtatagg ctatgcaaca aatggtttaa agcaatctga
    53641 gaataatgat gattaataaa aattaactga aaattttttc agtaataatt tctaaaaata
    53701 ctttttagtg taacgtaacg tgttatataa tactgccacc gaaacagtca aggttagtaa
    53761 cgcatttcca gcctttaaag aagttaatga atgaatattg taattttttt acagaaaata
    53821 ttttaatttg ttgctaaaat aataaaaatt atttatattt agtattaaat atatactatt
    53881 catttcttca aataaagttg tagtttatat gcttaataag gagggtgata atgcctacct
    53941 atgaatatgt ctgtacctct tgtaatgata aatttgaaat gtttcagtct ataaaggatg
    54001 aaccagtaaa aacctgttca aaatgcgggg gggaagttag acgtttaatt ggatctggtg
    54061 caggaattat cttcaaaggt tctggttttt atgtaacaga ttataaaaat aatggaaacg
    54121 gcaaaacttc aaaatcaact tctacagagc cagcttgtgc aacctgcgaa tcaggttcat
    54181 gcgcagcaga tttggactaa actaaataaa aagagtatat atctactgca gaatgcagtg
    54241 ggtatgtact caatatatag tattattttt tcatcatcag gtttcttttc tgtcgataga
    54301 gggtatgaag atacttctct atagctataa ttcctgatga cggtataatc tcaccattta
    54361 ttttttttcc aacaagtgaa tcacagaact ctttaattgc atcatacgaa cgaattacaa
    54421 caggtgaaac taaaatatat tctatatcag gtatcaataa tttttggctg cgatgcggat
    54481 acagcaccct acgggcatta tatattggta taacaatttt taaaccggta tccctcagtt
    54541 cttctattat ctcacttacc tgtgtttcgt tttcatcggt tatatccata acttcagata
    54601 atgcaattat atttctgttt attgaactta tagtatcata aaggtttctt gcctgcttgc
    54661 ttagttgatg tactggtgac gaaaatacct gtgcctgtgt aattggctta attatttttt
    54721 ttcttctaaa cagagaaagt attgcttcta taatacgtga aatcaatgat ttcttttgct
    54781 taatttcttt attaaccggt atttgagttg atatttcagc ttctttcttt tgatttttat
    54841 aatcatcaag aattttttgc aaaatttctt tttgttcttg atttaaagta agatcatcaa
    54901 tgttgtcagt aatgaatgtc tctgcaattt taatgagaat attataattc cttttcagct
    54961 tttctttttc aagatagcca agcttttcaa acttttcctt ttgataatac attaattgat
    55021 ttaaatctga aaggtaattt ataaatacag gaccatcaac ataataccat tgatcagttt
    55081 ctgaatcgtt gtatactatt atttttgcag ttttcctata attcgattca cgccacttcc
    55141 caatcaggga atcattaatt tctggtattg tgcttattat atcatcctgt aaacatattt
    55201 tatgctctcg tataactgtt acaatatcat tatgaaatgc atctactgga tcaagtaacg
    55261 aatcaacttt taataaacaa tttctgaatg cctgtaaatt ctttacttca gccagatcac
    55321 tttctgatag taatgaagcc agatcatcta tcagcacatt gaaaatatag tctaacttgg
    55381 catcacgata atcactgaaa tcatacgata taataccaaa tttctttaat ctacgcaata
    55441 tcatatctgc atgctgggct atgatgtttt tcaaggtttt aaaatttagt gttgatttaa
    55501 cagcctgata attgtctgca tgaaaacaat aatcctcagc taattcaata tggtttttat
    55561 ctatagtaaa atgtttgaag gtaaaaattg aattgcctaa ttcttgtttt attattctgt
    55621 tgagatcatt cttatttatt gattttttta taagctttga aagattaaat aaacgatatt
    55681 gttttaaaag tgttagcaaa ttctgtttag aaatttcatc ccgaattgac tgtaagcgat
    55741 taaattcctc atcttgaata tctacgcaat actcttcttt ttcattgata tcaagcttct
    55801 ttgtcttctc gtccacagaa aaactgaatg ttataagttt ttttacatga tcaggatagg
    55861 aattgagcaa ctgtgtaatt aaataattta tttgattatt tctggaggtt gtaaaaggga
    55921 ttggaatatt tcgtgaagat tgcaaaaatt gcattgttgc catttcccgt ttgccatcag
    55981 ggagcgatag ctcctgcatc ctaactattt ctgccagaat atcatataca tatgattctt
    56041 tagcaagtag cccctgaata aaaggaaaac gctgtgcttt attatcaata ctcatagtat
    56101 aatataagca tctgtaattt taaattagtc atattttacc catgtcatct caaaatataa
    56161 tatattggaa ataagtaaag tattttattg cgttaatttt ccctgtaaaa aagaaataat
    56221 tgaatcttgt tccacggtca tctgatcccc tgtggacata tcttttatag ttgcacactt
    56281 atttgccagt tcatcttcac caaaaattaa tgaaaatctt gcattttcac ggtcagcccg
    56341 ctttaactgt gttttaaatc cttttgcagc agggtcaaaa tcacatgaga tgttattctt
    56401 acgtaattct tctgctgtct ggataacaac aggtagtgca ttgtcaccag aatatgcaat
    56461 atagacatcc aggtgttctt ttacagataa ctgttcaagc aacaattcta tgcgttcaat
    56521 tcctgctgca aatccaacag cgggagtagg ttttccacca aacaactcaa ctaaattatt
    56581 atatcggcct ccagctgcaa aagcattttg accacccaat tttgtagtaa caaattcaaa
    56641 tgttgtttta gtataataat ccagaccccg taccagaaga ggatcatgaa tatagtgaat
    56701 ggaatgtttt tcaagaaaat tgctagtttg gctaaagtgt tctttacatg attgacataa
    56761 aaattgtgtg atttttggtg cattcttttt taatgctatg catccttcaa ctttacaatc
    56821 cagtaatcgc aatggatttg tacgtaaccg ttttttacaa tcttcacaaa gctcatgctc
    56881 atgtgtagag taatacgtaa acaactcacc aatatagggc ttgcgacaat cagggcagcc
    56941 tatagaatta atcagcaaat tatattctgt tattcccaat ctttgagcta tcgtattcat
    57001 taatgatata acctcaaaat cataccatgg gttatcgctg ccaaaaactt cagctccaaa
    57061 ctggttaaac tggcgcagtc tacctttctg gggtcgttct gccctgaaca ttggcccgta
    57121 ataaaaaaac ttacataaag ctaatctatt atattctccg ttttctacgt aagcacgtac
    57181 catggaagct gtcccttcag gtcgtaaggt caaacttctt ccaccacggt cttcaaatgt
    57241 aaacatctct ttggttacga tatctgtacc gtcaccaata ccacgcgcaa aaacttctgt
    57301 atattccatt acaggtagaa taacttcacg gtaattatac agtctgaata cttcctttgc
    57361 agtattaact atatgattaa attttggggt cctgtcagga aatatatctt ctataccagg
    57421 tggcttttga atcattactg tttcctccac aaaaatctaa aaatgcatta tagtcgtttg
    57481 cgtaaaattg cccattgacc caaaggtttg tcaaatataa ttgttgctat ggtattaggt
    57541 tgtgccattg atactgagtt accatccact aaaatatcga tgactgttac aggtgaatcg
    57601 tttatctgat tatttacaac tgggtatatt gcatcgtaac tatcgccttt gtatattggg
    57661 ttaaatactt taacaagatt tttttgctgg tcaatacaat atcccatgaa caacgttttt
    57721 ttaatatatg gcacaccatc aaatgtttga ttgttgaatt cgttaaaaag gtcttcagta
    57781 tatggcctgt ggctgaccag gtcaagttca ttatgccaga agtcactgta ccggttacta
    57841 tcgccaccat ttaaaaatgt atcaattgcg tgcctgtata tacgcgttac attggctgta
    57901 tagtacagtg atttcattct accttctatt ttaaatgcgt ttacacctgc gttaatataa
    57961 tcaggtagct tattaattaa acataaatcc tttgaggaaa gaatttcagt gccgcaggaa
    58021 tattcaatga tgtctaaatg attgccgggc cgtttttctt caaccagtga atacttccat
    58081 cgacagggat gtgaacagtt tccctgattg gcatcacgac ctgaaagata gcggctgagc
    58141 aaacacctac ccgaatacga aatgcacagt gctccatggc aaaaaatttc cagttcaata
    58201 tcagtgtgct cacgtatttg tttaatttct tcaagagtgg tctcacgcgc aagtacaata
    58261 cgtgatattc catggcttgc ccagaatttt actgccagat gatttaacgt tgacatctgc
    58321 gttgaaaggt gcaggggaac attgagatgc tctttgacaa gtaaagacat acccggatca
    58381 gaaaccatga ttgcatcaaa tggaatatgt tttatatgct gaatatattc tgtaacaaca
    58441 ggtatatccg actcatgcaa aaaagcattc agtaaaaata ttgcctttac gttatgagtg
    58501 tgggcatacg ctactgcctg ttctatttca tgtattgaaa aattttttgt ggttgctctc
    58561 agattatatt gctgaccccc aaaatacaca gcatgtgcac catagtgtac tgctgcctgc
    58621 aatttttcaa aattgccagc aggtgcaacc agttcaatat ttttgcctgt ctttaaagcc
    58681 attccttata cctttttgat aaaaatcaac agccctgtta tgctcagata acgtttttga
    58741 aaaatagtgc gaaccatcat tcctggaaac aaaaaacaga tatggcacct gtgccggatt
    58801 taacgcagca agtattgatc gtttccctgg attgcatatt ggcgttggtg gtaatcccgt
    58861 atatttatag gtattatacg gagtatctga atctaaatca tcataggtaa gacgtttacc
    58921 aaattttttt agtccatact ggatggttga atcgcaatcc aatttcattt ttaatttaag
    58981 cctgttataa aatactgcag caataagaat acgctcactg ttaatctttg cttccttttc
    59041 aactaatgat gctacagtta gtatttgatg aaggttagaa tactgtacct ctattgattc
    59101 ctgaatatca tggatcactt tttttgtctg gtttaccagg cgaattatta tatcacgggg
    59161 atcagtattc tcaggtatta cgtacgtatc aggaaataaa tatccttcag cacttggcgc
    59221 acgtattcct aaagatgcta aaaatgatgg tgactgagcc cagtataaaa aatcaccttc
    59281 tgtacagata tcatttgcgg ccaatactct ggcaatatca tatatggtgt atccttcagg
    59341 aattgttatt ctttttgtta caaatttacc tctggttagt tttatcaata ttgaaaagct
    59401 tgattcacca tcatatatgc gatagttacc tgctttaaca tcagtagcat taaggacgcg
    59461 ccccaggatg tagaaaaacg atgcactctt gataatattc ttcgatttaa ggaaatatgc
    59521 aacctgatga atgttcatac ctggtcttac ggtaatagta tcacccttca tggtgtaggg
    59581 agggctgtta aaaaaccaga atactattgc gaaaataata aatagtataa taaacgataa
    59641 tgcagctgct tttatttctt tcattatttt cctgatttgc accaaaataa atattttgtt
    59701 gactttacaa gctaagttgc tatctaataa cttttgtttt tactattact attttatcaa
    59761 ttaaaaaact aatgtgcaga aaaaatatcg ctatactact tttccttttc tgttgtgttg
    59821 ttctttacgg acaggaacca gtaaagctgc agcagaagaa tgctatctat tacaataaaa
    59881 aaggctggga aagacttgaa actggcaatg catattctgc aattgttgca tttcaaagtg
    59941 cccttgccca aaacccaaaa tataaagaag cattattggg attaggttac gcttacctgc
    60001 acactgaagg ctatactgaa tcagttaaaa tatttgatgc ggtgcttaaa ttgtatccca
    60061 ataatgagca ggcattaata ggtatggcaa aagcatatac tggtttaggt cgctatcgtg
    60121 aagcaatgaa aatctacgat acattaaatg agctatcgta caccaataat gattcacgct
    60181 atggcatggc atatctatac tataaaatgg gtaacatgct gtgggcaaaa cgcatgctta
    60241 accgaatttt gcaaaatgat ccatattatt acgatgcact gttactcttg gctgatataa
    60301 aggcaaaaga caaacgcttt gacgatgcca ttgatctggt aaaaaaagcc atagatgcaa
    60361 aagaaacata ccctgatgct tttgtcaaat tggctgagct actgtatctt aaatattatt
    60421 attctggcga taaagcatat ttacaacaag cagttgatga attacagaaa gcgttaatta
    60481 taaacaaaac acatctgcag gcaaatcgcg atatgggata tttgttgtat attcaggggg
    60541 attatcaaaa tgcaatttcg tattatgagg cgataattac tcattatcca catattgcca
    60601 gcaatgaaat cctgtataat ctaggcttaa tgtatgaata caaaggcgat agtaaccagt
    60661 ctctaacata ttaccagaaa gcactatcaa aagacaacct taatagcctc attgccatga
    60721 aaatgcgaca gctttgctta ctggaacaca ttaagatagg ccatcctgta cgaatacaga
    60781 gcagtaatga tgattttagc tcatcacagg aattacgaaa aaaaggattt cccgatttat
    60841 ccatgttgtt tgtccgttta tcattggcac tcaatcccaa taataccacg gcacgtcagg
    60901 agttaatgcg tatatatgaa actctgggtt attttgagtt ttactatgat gaaataaaaa
    60961 aaatgcatac attaaacccc acacaaaaaa cacaggatat gatgagtatt gccgtaatga
    61021 agcgaaggga caagctgttt ttccgtttag gctacgataa agaagaattg ccacgagatg
    61081 tgccactggt tctggttctt gattttacac ctactgttgc agtacctgca tttgccgatg
    61141 cgggatctgt aatagccaat tcaatcaact ttgccatggg gcaatttgga cgttacagaa
    61201 acttcccttt atcacaacgc caggcaatta ccaaagagtt tgcgtcaaat aatttagacg
    61261 atattttaag tatactcaat gataaaatta attccaatga acttcctcaa atatcgtaca
    61321 tgttatatgg agaatactca ctgtcaggaa gatttattga aattaatgcc aaattgatgg
    61381 atgtaaaaac tggagtagta atagctcact ataacgaatc ggacaatacc aaatattcat
    61441 tacaaacggt atctctggat atagccaaaa atttgtatag caccattccg tactggggta
    61501 aaattcagtt ttactcagat gatatggtgt cagtaaacct ggggagtata gatggtattg
    61561 aaatagacag catgcttgaa acatttgtag ataatgaaga taccattgat tccgaaaaac
    61621 cccgaaaaaa agtaatatta caggtgaagg aagttgatac cagggttctt ttagctaaac
    61681 ctataaaccc taaagatatt gacctgcttt caaaaggaca ggaagtgtat cctgtttcaa
    61741 aacgacgagc aaaaaaaata aattagtcag tatataaatt tatataatga aatatactaa
    61801 taaatcttga catataaaat gcaatttata ccattgtgca caaatgggct tattctaatt
    61861 tttttgcata aaattttata aaagggagtg ggactatgaa gatagctgtc ttagttaagc
    61921 aggttccaga tatggaatcc aaattcaaaa ttattggcga cagtaagatc gacgaatccc
    61981 agattgcttt caagatgaat gattttgatg aatatgctgt agaggctgcg ctgcaattga
    62041 aagaaaaatt tggtggtgaa gtaattatca tttcagcagg ccctgagcga gcatcaaaag
    62101 aaattcgtca ggcatttgca atgggtggcg actggggtat ccagattcag gatgagcagg
    62161 ttgatgcagg tgacaatttt gtggttgcat cagcactgtg caaagctgtt gaaagcttgg
    62221 gcggtgttga ccttgtactg accggtgttc aggccgaaga tgaccaggca gcagtcactg
    62281 gtgttatgat tgctgatatg cttggcttgc cacattgtac aaatgttgtt aaaattgaag
    62341 ttggtggcga tggcaaatca atgaccgtta accgtgagct tgaaggtgga ttaaatgaag
    62401 tacttgagat ggctatgcct gcagttctta caattcagtc aggtattaat cagccacggt
    62461 atccgaccct tcctggtatt atgaaagcta agaagaaaag acttgatgtc aagaaggctg
    62521 cagatcttgg agtatctgct gttggtgatg ctggttcaaa gacaaaattt gttaagatgt
    62581 tcttcccggt atcagatcac aaggcagaaa ttattgaagg tgatccaaaa accgctgctg
    62641 ccaagcttgt agaaaaacta cgcaatgaag caaaagtgat ataagggggt acacaatggc
    62701 acgtatatta gtaatagcag aacagagaaa cggaaaaata agcgatgcta cattagaact
    62761 ttgcaaagct gcaaaggcat tagcatctgc aatgggtgca tcccctgctg ctgcagtatt
    62821 ctataaagat gacagtttag caaaggaagt tgcacagtat attccagaag tatttgcagt
    62881 agtagatcct aaacttgaag tttatagtgc tgatacgtat gttgctgctg ctaaggcagt
    62941 ggtagaagca caggatgtaa agggtgtgtt agtaccccat acatacgatg gtacagatta
    63001 tgccgctaaa ttggcattga atttaggatg tggtatagtt acaaactgca acaaatttga
    63061 agtgcagggt ggtgccgttg tatttacacg taacacctat aatggcaaaa tacaggaaca
    63121 gagatcagtt aaaaccgata agtttgttgc aacatttgaa aaaggtgctt ttgaaaaaga
    63181 agctgcaggt ggtgcaggtt cagtaactgc agtatcagct tcaattcctg aaccacgaag
    63241 aaaatttaaa cagtttgtgg aaactatggc tggctcagtt gacatttcac aggctaagat
    63301 catagtatcc ggtggacgtg gaacaaagga aaaggataaa tataacgata tcatcgtaaa
    63361 ttttgccaag aagctcggcg gtgaatatgc tgcatctcgc cctgttgttg atgctggttg
    63421 gacagacgct gcacgtcagg taggacagtc aggaaaaact gttactccag atctttatat
    63481 agcttgtggt atttcaggtg ctatccagca cgtagctggt atgaaaggtt ctaaagttat
    63541 agttgctatc aacaaagacc ctgaagcacc tatttttaac attgctacct atggtatcgt
    63601 gggtgacctt tttgaagtaa tacctgcgat tatggaacag ctatagtaaa cagtaaaatc
    63661 gtattaaaaa aggggtgtct cccaaaagac acctcttttt ttaattaatt tattaccata
    63721 tactagatat tgataatctg aaaaaattta aaaatactat caaaaagaat catagtggat
    63781 actgaattca gcaggaagaa aaattacaaa caacttgata ataaggagat tgaaacgttt
    63841 gtgaagttta attatttata aaacaaggcc gtcacatggt ttgatatgca acggcctttt
    63901 ttaatttata tgctcatacc agtgttaaag aaaaatttta aagttgctgc aaaaccaaaa
    63961 tcccttgaat tatttaatcc ttttgcaaat tcattttgaa aatatacatt gcccagatta
    64021 aatccaataa aaaggcttgc tgcagcttcc ccttcagtaa aacgtttacc caatgcactg
    64081 ctggattgag cagatgcaaa ttctccacca acacctataa tacctatatt gtttatatta
    64141 taaaacaaac caccatgtcc accaaatgta tgctctctgt catttttact ataggttaat
    64201 tcatatactg gatgaataac aacaatgaaa tttgacagat acaaaagatt tggtatagaa
    64261 aaatgtgcca tattttcatc aatatctaca cctgtatgat gctctgcaac caaatccata
    64321 aaataatttc cactatttga acggattgta ttaaatacac ctcttactga ccatgattct
    64381 aatgaatatt tgttttctgc acgtgtggtt tctgcctgag ttaagccaaa ctgaactgaa
    64441 actctttcgg ccaatccata atacatactg taattggtaa aatatgaatc ctgatcggga
    64501 gcatcagtat cgttgaatga atattcaccc aatacatctg ctgcaaaaaa accagttgga
    64561 tacaccatag atttatgatg taagaataaa aaagtatcaa aacatgcata tcctggctgt
    64621 atcaaaaaaa tagatactgc cataatagca actataataa aaaaccgctt aaacataata
    64681 caccctcctt taaaaattag tattaaatga atactaaaat catattaata gcactttatg
    64741 tcaataatat ttttatatat ttataagata atattaatta taacttagtg ttattatata
    64801 gcaattcctt agtttttata tgtatattaa aataatgatc aataaacaat aatgtatgga
    64861 aaagaagctt ttgtatatct attgaattat atatatcagt ttcaatattt agaaattttt
    64921 tagtcagaat ttcataaatt aatggagctg ttgccttaaa aacaaatgca ttgttaggtg
    64981 cacattggtt acaatacgaa ataagttggt attcagttaa atacaggtca tcagtaattt
    65041 ctttatggca aactgaacaa tgtaagtaat caggtataat ccctgataat ttaagataat
    65101 gaagaataaa agaaagagaa agaaaagtat atgcactggt tttatgtaat gtttcgatag
    65161 ctccttttaa tagcttatat attgtaatat tcgggtcatt gtgaccagtg gttttttcaa
    65221 caacctccag tagtaaaaaa agggtaatca ttgcctgata attatctcta attgataccg
    65281 tatccagtaa aagcgaaaat tcctttacat gatacattgc tttttgttca ttgtaatagt
    65341 atacaaggtg tacaacacta ccaggctcta ttgcagcctg gctgcgccgt tttgtttttt
    65401 taattccctt aaatataaat gtccgtttac catggttctg ggtatagata atgtctgcaa
    65461 catctgcttc caggacagga atttttttta atattattcc ttcagattta atgatttcca
    65521 ttgaatttta ataggtatct gaattttttt gtggggtaac tgttacaata atcctgccaa
    65581 tgcgagtacc ctgtatatct ttaattttga atgttacatt attgtatttt atttgttcgt
    65641 ctttctttgg aatctttcca aaaaggtcaa aaacaaatcc tccaattgta tcaaattcct
    65701 gatcaggtat agcgcaacca gtatgttcat taaaatctga tattgataac cgggaatcaa
    65761 cctcccatgt attttttgaa attgtctgta tttcaggaat ttcatcttca tcaaattcat
    65821 cccgaatttc accaacgatt tcttcaagga tatcttcaag tgttacaatg ccagcaaccc
    65881 caccatattc atcaacaact atcgccagat gtaaattttg cttctgaaac tgtagcagca
    65941 gttcatctaa agtcattgtt tcaggtacaa aatatggctt gtgcaaaagc ttctttatat
    66001 tgaatttcat tcgcctgtcg ataataaatg gcataaggtc tttaacatac aaaatgccag
    66061 taatattgtc gatagttcct gaatatacag gtatacgtga atgaccttcg tcaataacac
    66121 gttttaccag ttccttcaac tggatagaat cctcaacagc attgatatca actcggggta
    66181 tcattacttc ccttacattt ttggtggaaa ggcttaaaat gccctgaatc atctcctgtt
    66241 tctggatggc taacttatca actggaacag atatattttt tttatgtata aatttaaaga
    66301 atttttgttt taataaagat actgcatcat tattattagt ttttttgggc ttttgctcat
    66361 ccgaagccat agtaaatata attattttac ctccttttct tataattcag catagtacca
    66421 atgtgactgg caaattgtca atttgatttt taccaggtgt ttattaccag aagataagtg
    66481 gcatttttaa ttttaaaata cacttgacaa taaacggtat ttcttttttt tagacatcaa
    66541 aagattactt tattatttta attgttttaa cattctgaat taaaatttat aaaaaggagt
    66601 ggccctatgg atagtatgct tttttggatt gcccctgtcg ggtcgatcat tgccctttta
    66661 tttgccttca tcttttataa aatggtgatg aaagcagata aaggaaatga aacaatggtc
    66721 gagattgcgc aggctgtacg cgaaggggct tttgcttatc ttaagcaaca gtacaaggtg
    66781 gtctctttgg tattcgtgat cctggtaatt ttattgtcta ttcttgcgta ttatgggatc
    66841 cagaatccat ttgtaccttt tgcattctta actggtggtt tcttctctgg tttgtgcggt
    66901 tttcttggca tgaacactgc aaccaatgct tcatccagaa cagctcaggg tgccagagaa
    66961 tcactgaaca gaggtttaca ggtagctttc agatccggtg cagttatggg tcttgttgta
    67021 gttgggtttg gtttgctgga tatttcatta tggtacatta ttttaaactg gatctacgac
    67081 aacaatatca tgggatttag ccaggagttg gctgaaaaaa taggactttt gactgctgga
    67141 cagtcatttg atgtatcgct tattactaat gaagccttca aacatggcaa gcttgttgaa
    67201 attaccacaa caatgatcac atttggtttg ggtgcttcaa ctcaggctct ctttgcccgt
    67261 gttggtggtg gtatttacac aaaagctgca gacgttggtg ctgaccttgt tggtaaagta
    67321 gaagcagaaa tcccagaaga cgacccacgt aaccctgcaa caattgcaga caatgttggt
    67381 gacaacgttg gcgacgttgc cggtatgggt gctgacctgt atgaatcata ttgtggttca
    67441 atactttcta cagctgcatt aggtgctgca ttaccacttg ttggtattga aaatgttaac
    67501 gcagttattg ctcctatgat cgtagctggc ctgggtatcc tgttctcagt tattggtatc
    67561 ttctttgtta gaaccgaaga agatgcatct atgaagacac ttcttaaggc tttaaaccgc
    67621 ggtgtatggg gaagctcaat acttctgata attgcattgg cagctcttgc ttatgttaat
    67681 ataatatcat ggggtgtatt tggtgcttct attgctggtt tgattgctgg tttagtaatt
    67741 ggtaagtcta ctgaatatta cactgctgat gattattcac caactcaggg aattgcaaag
    67801 cagcatacta tgggaccagc aaccggtatt cttgatggta tatctgttgg tatgatgtca
    67861 acagctatcc cggttgttac cattgtattg ggtattttgg cagcttttgg tctggcaggt
    67921 ggattttcat ctgatccaaa cgctgtttca aaaggcctct atggtatagc ttttgctgca
    67981 gttggtatgc tttcaacatt aggtataact cttgcaactg acgcttatgg tccaattgct
    68041 gacaatgctg gtggtaatgc cgaaatgagt cacttaggaa aagaagtacg aaccagaact
    68101 gacgctcttg acatgttagg aaatactacc gcagctatag ggaaaggatt tgcaattggc
    68161 tctgctgctc ttaccgctat ggctcttatt gctgcttaca ttgaggaagt aaaaatgtgg
    68221 gtaagcaaat atacagcacg cagcgccgaa ggtttcttcc aggttggttc agtaaaattt
    68281 tataatatat tgccggaaaa tattactgct gatctcaata atgttgtaat taaacatgca
    68341 acaattggcg acttcgtacg tgcatatgac atcacactgc tcaatcctat ggttattgct
    68401 ggattgttcc ttggtgcaat gatggccttc ctcattgctg cacttacaat caaagcagtt
    68461 ggaaaagctg ctggtgctat ggttgaagaa gttcgccgtc agttcaagga attccctggt
    68521 attttgaagg gaacacagaa accagattac gcacgttgcg ttatgatttc aacagcaggt
    68581 gcacagcgta agatgattct tccttcaatg ctggctttaa ttgttccaat agctgctggt
    68641 ttactgctgg gagttgccgg tacaacaggt gttctggctg gagctcttgc tgcaggattc
    68701 tctctggcat gtatgcttaa taatgctggt ggtgcatggg acaatgctaa gaagtatatt
    68761 gagaagggta actatggtgg aaagggttca gacccacaca aagctggtgt tattggtgac
    68821 accgtaggtg acccattcaa agatacttca ggtccatcac ttaacatact ccttaagctt
    68881 atgagtatgg tatctgtagt atttgcaggc gttgttgttc acttctatcc acagattgca
    68941 aaattcttgg gtatgatgaa gtaataaaca aaaacagggg gctcgagccc cctgtttttt
    69001 attcatgata tacgggtttc cctaattctt taaaagaaaa aagcttaaaa gctcgtgaac
    69061 catcataatc cagtattcgc aaatcatcag tttcatgtaa atctctgatt acaatatcaa
    69121 aatgaccacc atatcttttt tttgcttctt ctactggtat ctcataagca atgaatttag
    69181 taattccctt agatgctaac ttttctttca gtttatcatt cataaagcta tcataagaag
    69241 ttaaataaat caaagcacca ttacctgtta tgagtaaacc tgctttcatg gcacacctcc
    69301 ttataattat attttgtaaa ataatataat aatattttat attaaaatca ataaatgaaa
    69361 aaataatacg aaatcacccc taaacaattg gataaaagag ggattgaaca ccttagtcat
    69421 atttggacat ttaagtgtac accaaagatt gttttgttta tatatcatgg tgaagaataa
    69481 atgctggata cgtgtatttt ttattcctgg caatgatttc ttaaaagttg ttttttatta
    69541 tgactgtatt atctcaagtt taagttgaaa gatatgtaaa atctaactat tttatttatt
    69601 atagtgtgtt aactttatta tttttttggt aaacatgtat tatattgata taatgaatac
    69661 atgcaaaaga tttatttcgt atctttagca ttgaacaaaa ctgatacata ccgagcagct
    69721 aaagatagaa tcaaaaaagc gcaccattta atggtgtgct tttaatttta aagcttgatt
    69781 tttttatatt aaaaaaaata ttgatggaaa aattagctta caagaggtct tgctatggaa
    69841 ttagcccgtt acaggattga aatatattat actattcccg attctcgtgg tacagtactg
    69901 caacaaaaac tacaaagatt gggatttcct gtaagcaaat gtattcgtac cgataactat
    69961 ctcataaata tccctttgaa tgatagtcaa attgagaagg ttgcacaggc gcttgtacaa
    70021 cctgttacac agaattatac tatcaataaa ccatacgcac cctctgattt taggtatgct
    70081 gttgaaattg gatacctccc cggagtaact gataatgttg ctcacacaac ccgtgaaatc
    70141 attgaagata tttttaaaat acaatgtgat cctgaaaaat gcgtgtttac ttcatctaca
    70201 tatttcttct gggggggatt gacaattgat gcaattcatg aaatatcgct tgagctatac
    70261 aatccgttga tacagaggtc attaatttta tcatatgacg agtacatcaa aaatggtggt
    70321 atgggtaatg ccatacctgt agtaacactg cacgaggata caaaggcaga agttgtttca
    70381 ctggatgttc ctgaagacga acttatagca ttgggaaaag agggtataaa aaatccagat
    70441 ggtagccgtc gaggaccttt agcactggat atgctttccc ttgaggcaat aaagaaatat
    70501 tacagggata ttgaaaaacg aaaccccaca gacgttgaac tagaatcaat agctcaaacc
    70561 tggagtgaac actgtaaaca tactattttt gctgcacaac tggatgacat caatgatggc
    70621 atattcaagc attatatcaa agaagcaaca ctgagaattc gccgcgaaaa gggcaaacat
    70681 gatatatgtg tttcagtatt ttcagataat gctggtggaa ttgaatttga tgatgattat
    70741 gttatagctg ataaggttga aacacacaat agcccaagtg cccttgatcc ttttggcgga
    70801 gctattaccg gtattgtagg cgtaaatcgg gatgcaattg gatttggatt gggttcaaaa
    70861 ccggtagcta accgctatgg attttgcttt gctaatccct ttgacacaaa gcctttatac
    70921 aaatcaaaag atcccaattc taagatgcta tcgccccgac gtattatgga gggtgttata
    70981 cacggtgtta atgttggtgg caactgctct ggcataccaa caccacaggg ctttgtgtat
    71041 tttgatgatc ggtacaaagg gaaacctctg gtctttgttg gaactgttgg cctgattcct
    71101 aaaaagatta aaggaaaatc ttcagtacat aaaaaagcaa tgcctggaga taacatagtt
    71161 atggttggcg gcagagtggg gcgcgatggc attcatggag caacattttc ttcggaagct
    71221 ttaacatcag gcagtcctgc aacagcggtt cagattggtg accccattac acagaaaaaa
    71281 ttttctgatg caatagtgaa agaagcacga gatttagggt tgtatcactc aataaccgat
    71341 aacggagctg gtggactttc atgttcggtt tccgagatgg cacgtgaagc tggcggtttt
    71401 attgtatatt tagaaaaggt tccgctgaaa tatcctggcc ttgcgccatg gcagatatgg
    71461 gtatcagaat cgcaggaacg tatgacctta tcagttcccg atgaaacgct tgaagatttt
    71521 cttaacctga tgaaacgccg tggtgttgaa gcatgggtaa ttggcaaatt tacagattcc
    71581 ggaagaggaa ttgtgtatta taatggggag atagttttta acattgacct tcattttttg
    71641 catgaaggcc tccctcagaa aacgctcaca agcacctata ctccaaagtc ccatccatat
    71701 cctgagtttg atgatccgga aaatataacc gaacatttta ttgcaatggt taaacgctta
    71761 aatactgcaa gctttgaatt tatttcaact cagtatgacc atgaagtgca gggcaacagt
    71821 gtaattaaac ctctgcaagg taaagggaag gtcaacggaa acgctactgt tatacgacct
    71881 ttgttggaat caacaaaggg agtggttttg tcacagggat tatatccttc atacagtgat
    71941 attgatacgt actggatggc agcctgttca atagatactg caattcgcaa tgctgttgct
    72001 accggatgtg atctggataa acttgcattg cttgataatt tctgctggtg tgattctaca
    72061 aatccataca ggttaggcca attaaaagaa gctgcacgtg catgttatga ttttgctgtt
    72121 gcgtataaaa caccatttat cagcggcaaa gacagcatgt ttaatgattt caaaggttat
    72181 gatgaacatt ttaatgaggt atttatatca gtaccgccaa cattgttaat ttcttctatt
    72241 ggagttgttt ctgatatttc ttattgtcaa actcttgatt ttaaaaatgc tggcgattat
    72301 gtatatatac ttggcattac cagagatgaa ttgggtggga gtgaatattt agcgtatatg
    72361 ggtgaaacgc tttctgggga aagatatatt ggcaatacta tcccgcatgt tgataccatc
    72421 atgaatacta aagtatatag agtaatagaa aaagcattac gtgcaggctt gattgcttca
    72481 agtataagca ttgagcgggg tggcatagct ctggctatag caaaatcggc aatgggcgga
    72541 ttactgggag caaatattga tttacgtgtt attccacatg aaaatattaa tcgcaacgac
    72601 acactacttt tttcagaatc acagggaaga attcttgtgt cgatagctcc tgaaaataaa
    72661 actcaatttg aaaaattatg taaggacata ccttttgcta agataggaca ggtcacagat
    72721 aattccttga ttagaattaa tgctttaaat ggaaatgaaa ttgtttcagc ttcagttatt
    72781 gatctgcatt ctgcatacaa atctactttc atcgaatttt agaggtaaaa atggcaaaac
    72841 caaaagcctt aatattaacc ggatatggca taaattgtga tgaggaaaca cgtttttcat
    72901 ttgagatagc aggtgcacat gcaacaataa ttcatataaa tgacattatt gaaaataaat
    72961 cacttctaaa ttcttaccag atttttgctt ttcctggtgg attttcgtat ggtgacgata
    73021 ccggcagcgg caaagcactg gcaaataaaa ttaagtataa cctttatgaa gaaatgatgt
    73081 catttattaa ccgtgacact ttaatgatag gtatttgcaa tggatttcaa gtcatggtta
    73141 atttagggat ggtacctgca ttgacacaac caattggtac tgttgaagtt tcgcttgaac
    73201 acaatgctac attcaggttc cagtgcagat gggttgatct ggccatcaat tctcattcca
    73261 actgtgtatt tactaaaaat atggaaaggc tgcatattcc agttgctcat ggtgaaggca
    73321 attttgtagc accacagcat atcgttgata tactttctaa aaataatcaa atcataatgc
    73381 aatatataaa gccagatgga tcatttgcac agggtcaatt cccctataat ccaaatggtt
    73441 ccattatgga cattgcagct atttgtgata caacaggcag aattatgggg atgatgcctc
    73501 atcctgaacg ccatatatac tttatgcagc gtgatgactg gacattttta cgtgaagtat
    73561 caaaaaggca aaaaaaagaa ttcccacaat atggtgaggg attacagata tttaaaaatg
    73621 ctgttgagta ttacaaataa acaaaacaca tttacggtca tcataatatc ttagataata
    73681 ttttcatcag ttagtgtgtt gtagttatag tattttttca tttcaataaa agataaatta
    73741 atttgacttt gattattttt acatttatac tctttcacca gagcctatca aataaaagcc
    73801 tatgtataat aatcctataa taatcattat atctagatat gataaattat atagcatcat
    73861 ttttgcatga acttttgtct atcccaattt caaagaaaaa gctgttctat ataataataa
    73921 tcttttctgt aataatggtc atcggggtta tattactgta cttgcaatat agtgaaatta
    73981 aatctattgc atataataga cttttagcta taactgaaaa taaaaaagaa gatataagta
    74041 gctggttgaa agatcaaact gacgatgcag atttactgat acaatcaaga caatttagaa
    74101 atctattaca aaaatatttt acgtataaca atgctgaaag ttataataca ttatatgaat
    74161 tttttgactt ttatgcaaaa aacaacgata tcagtgaagt attgctggtt aataaagatg
    74221 gcaccatttt attcagctta tataaacaca taaaactgtt atcacaagac actcttcaat
    74281 atctgcaata tgcagctaat actaacaaac cagtatttac cgatatacac attgacaacg
    74341 atgatttcaa ttatcatatt agcttaattg ttcctgtaaa tcctgatttt acggcaagat
    74401 caaatcattt tatcatattg atatcttctg ctgaaaactt tatctttcct gtattacaac
    74461 gatggacagg tcttacacaa accggtgaaa caattctgat tgttaaagat agaccaacgt
    74521 tatatatatt taaatcatat ttagcgcaaa aagttccaga gattataaaa gtaaatacag
    74581 agtcactatc gcatgataat gtcattcgta tggcaataaa cggtgttaaa ggatataaat
    74641 atggaaagga tttaaaaaat atacatgttg gaacctatat taccagtatc cctcaaactc
    74701 cctggattat cttaaccaaa tccagcgaag cagaattgtt tcagagttgg tacatcatag
    74761 tcactatatc gattgcacta ttcatattaa gcattatatt attattttca ataattacta
    74821 taatgcaaaa agaacagcaa cgaacccttt accaggaact atcgcaaaaa caagacgaac
    74881 aattaaaaat ttaccagatt tataaatata ttatacaatc aattggtaat ggacttatta
    74941 ttacagataa ttcaggaaat attgaattta ttaatacaat cggggaatta ctgacaggat
    75001 ggcataatga tgaagcagag ggtaagcatg tacttgatgt tttacatata ctttctcaga
    75061 ataatactat aacttcaaaa gatgatttat tccagattat cgctgaaggt aagttcagag
    75121 gtgaatatca caatagtgtg cttatttcta agactggcct tgaaatacca gttaactatc
    75181 atggaacgat attaaaatct ggacataatc aaaatttagg tgctgttatt gttatagaag
    75241 atcaaaccag ggaaagtttt ttaaatctta ttaatgatat acggctaaaa tttattaatt
    75301 tttcaattaa tcatacaaaa gatgatttta taaaaaatgt gctccacgag attttaacaa
    75361 ttactaatag tcagataggt tgctatattt catataaata tctttataaa cagataacag
    75421 gtttaaaaga attacccaat tcgtatcata cgtattgtat tgatcaaaat attgattgtt
    75481 cagtagatta tgaaaaagtt tggaaggaat gtcagaaaaa taaagggcat attattaata
    75541 atgaccatac gcagtatttt tattgtgaag agcttaaaaa tggtacagac cacccgcgcg
    75601 aattatttgt gcctgttttc agggaaaacg ctattgttgg cataattggt gtagctaata
    75661 aacacaaaga ttatacatat gctgatgttt caaaactatc catactctcc gatatggtat
    75721 atgagatact atctcataaa gaaaacgaag aagcatataa aaatttagta atgctatcaa
    75781 atgagttagc atgcattgca gatttaaaga ctacccgctt tcttttagtc aacccatctt
    75841 tttcaaaaac attagggtat tcagaagaag aactaacttc aaaatccttc cttgatttta
    75901 ttcatcctga tgataaagaa aaaaccatac aaattattaa tgaaaaactt cttaaagggg
    75961 aagacgttat tacatttgaa aatcgttata agtgcaggga tggaagttat gtatggttag
    76021 actggaattc ccatcctgtt atttcgcaag gaataacatt tgcaacagca cataatatta
    76081 ccgaaaggaa aatagcagaa gaggtattga aagaaaatga aaaaaaattc cgtgaacttt
    76141 ttgtacactc caatgatggg atatgtttac atgaacttgt ttacgataat aatggtaatc
    76201 caattgatta taaaataatc gatgttaacc cacaatatga agatatatta tcgttaaata
    76261 aggaagatgt cattggtaaa ttagccacag aaatatataa tacatctgaa cccccatatt
    76321 ttgaaatata taaagaggtt gccgtaacgg gaaaatctac aacatttgaa acatattatg
    76381 aaccaatgaa taaatttttt attatctcgg ttttttcacc acaaaagggg caatttgcaa
    76441 ctgtttttca ggatattacc gaaattaaat tagctgccaa aaagatagaa gagtctgagg
    76501 aaaagttttc cctgtttatg aaaaatctgc ctttagtggt ggttatacgt gatatcaatg
    76561 gcagatacat ctacttgaat gatgaatggg aaagagtaat ggaattaaaa aaagaagatt
    76621 ggctgggtaa aacaccatac gacgtttttc caaaagaaga tgctgaaaaa ttattacagg
    76681 gtgatagaga agcaatagag aaaggtgtca cagaaccctc agagtttact ttgaaacata
    76741 aatcgggtat taaatggtgg atggcaaatc gatttgtttt gtatgatgaa aacaaaaaac
    76801 ctgcgtacgt tgcaacaatt tatattgaca tcactgaaag aaaaaaagca gaggaggaaa
    76861 gagaaaagct taaagatcaa ttgatacagg cacaaaagct agaatcggtt ggcagattgg
    76921 caggtggagt tgcccatgat tataacaata tgctggaagt catactgggc aacgctcagc
    76981 ttgctttaat gcaaattgac agcaaagaag atgttaaaaa ttatttagaa gaaataacaa
    77041 aggcagccaa acgttctgca gatattacaa aacagctttt gacatttgcc cgcaagcaaa
    77101 taagtgaacc acagatagtt aacataaata caataattga aagcatgctc aaaatgttac
    77161 ggcgcctgat aggcgaagat atagatttac aatggatttt aagtgaaaat ctatggaatg
    77221 tttatattga tcctgtacag gtaaaccaga ttattgccaa tttgtgcgtc aatgcacggg
    77281 atgcaatcca gaataatggg attatcacta taaaaacaaa gaatgttaca ttggatgaag
    77341 aatactgcaa agatatccct gattgtcttc ccggagaata tgttatgcta tcagttagtg
    77401 acaacggtat tgggatagat aaagaaatta ttggcaaaat ttttgaacct ttttttacca
    77461 caaaggaagt aggtaagggt accgggcttg gcctggcaac agtatatggt attgtaaaac
    77521 aacatcatgg ttttataaat gtatattcag aaaaaggaaa aggcagtact ttcaatattt
    77581 atttacccag acatcgtggc ctaaaggaag ctgaaaacag tgttactggt aatagctggg
    77641 tatatggaaa tggtgaaaca atacttgttg tggaagatga accttcactg ttaaaaatgg
    77701 ttcaggtcat gcttgaaagg cttaattata cagtattatc tgcacaatcg cctcctgaag
    77761 caataaatat tataaaatca aaaggacaat ctattcaatt aatgatcact gattttatta
    77821 tgcctattat ggatggctta caacttgccc aggaagttgc aaaattatca ccgcatataa
    77881 aatttttgtt tatgtcaggg tatactgata gaatattatt tgagagtaac agtagtaata
    77941 aagaaacaaa ttttttacac aaacctttct caataagtga actggcaaag aaaatacacg
    78001 atattttaga aaacaaataa cccttccata ttctttcaag gaagggttat ttagtgtaga
    78061 tacaattaaa tattttatta atataattaa aaattcatct tcttccctaa catatattta
    78121 cctgattggg cttcatcaag cgttccatca gccttgtaaa atttccattc accattcatt
    78181 tttccgttta aatattctcc ttcagatttt attttcccgt ttggataaaa ttctttgtat
    78241 ggtccattta atttatccat taaatacgtc ccttccaatt ttacgctttt agtcccggta
    78301 taatattcag tccatttccc atttttcttt ggagtttccc atttacctgt ccaggtatat
    78361 gtaatctttt cttttggatc atcattaggt acagatgagg aagtcatttc cccagtgggt
    78421 ttcccatctt ttagtaccgt gtagatacct ttaggaattc ctgttaactg accataccca
    78481 atgagttgac ctttctcatc atataacttt gcattttcgt ttgtattatc tatcatacca
    78541 ccttgaagag gaagttccat tatctttatt ctctgcaccc gtgaaggata catgtagaaa
    78601 ttccataatc caatctcctt accatcttta tattttcctt ctttttgtaa caggccttct
    78661 tttgaattga atttccagtc tccctcaggt ttatccatta taaaacttcc tgttgctgaa
    78721 atctgcccat tatcaaagta aaatttaaaa ttaccatttt tcttgttttc tttatatcta
    78781 ccttcttcta gtttttttcc attttcatac catttgttgc actgcccatc taattgccct
    78841 gctttatacc atgcgattat cttgggattt ccattttcat aatactcctg acttcgaccg
    78901 gtttgctcac cattggtaaa ttgttcttct ttccagattc ttccagattg gtaataccat
    78961 gtttttatgc cttctatggc gccaactttc atggtaaatc cactttcagt tttttccttt
    79021 actacatagg tagctttcca catgacaggt ccttcaggat aataggctat ccattctccc
    79081 gtcttctgat cctgatcaaa gtttccctct aaacttttag caccattttt atggtaatac
    79141 gtccagaatc cctgctgctt atcatcaaca tattcacctt cggctaattt ttcaccagtt
    79201 cctttatgat ataaaatcca tttaccggtt ttcacgccat tggtaaaatt tccttcacct
    79261 tttaaggatt tatcatccca gtaaatcttt cctgggccat tttttgtact tgcggcgtta
    79321 gtttgccctt tcttatcagt gctggaacat gctgtaaata gcaatacaat agcaagtaat
    79381 gcaaatacac gtttaaactt catagttttc tcctttttta tttttataat gtgtattatg
    79441 tatgcaattt aagttacaaa aattatctat atattcaacg catgtatcct tttatatctc
    79501 atttgtcaat catttatgtc aagcaatatg ataaatatgc cgaaaagtac ataaaagtac
    79561 aatattttac tattacgagg tgtcgatatg atgcgttcat tgtggacagc tgcatcaggg
    79621 atgataggac agcaatttaa tatagatacg atatctaata atttatcaaa cgttaataca
    79681 acgggtttta aaaaactaag ggctgaattt gaagatttaa tctatcagac cgtccttatg
    79741 gccggtacac ctgctaccga gataagtgaa gtaccaacag gtatacaggt tgggcatggt
    79801 gtaaaggtgg cagcaactca gaagcttttt gatcagggaa gtttacaggc aacggggaac
    79861 aaacttgata ttgccctgga aggtgaaggt tttttcaaag ttcagcttta tgatggtact
    79921 attgcatata cacgtgatgg atcatttaaa gttgattcaa acagacagat agttacttca
    79981 aacggatatt tactggaacc acccattgtg ttaccggaaa attttattat ggaaacactt
    80041 tctatcagtc aggatggcag aattaccgtt aaagttgttg gcgatgatga acctattgaa
    80101 gttggtcagt tagaaatagc ccgctttgtt aaccctgctg gcttacagaa tattggtggt
    80161 aatctatata aaacaacacc tgcatcaggt gaagaaattg cagggcctcc gggaattgac
    80221 ggtatggcac gcacacatca gggattttta gaaatgtcta acgtaaaagt tgtcgaggaa
    80281 atggtaaata tgattgtagc tcagcgtgcg tatgaaatta attcaaaagc aatacagacg
    80341 tctgattcaa tgttaggtac tgctattgga ttgaagcgat aatgaataga ataattgtag
    80401 caactataat tatttcagca ttgttccaat cattgctgta tgcagatctg gcagtatatt
    80461 taaagtcaaa tactgcacca aaaaataatc tttctatttc tgatatagct cagattgatg
    80521 gcgatgaatc tgaattggtg aagaaaataa ctatacctga atctgttctt gacgatggat
    80581 acatcactaa aaacgaagtt tataaattat tgaaagatgt tgttacggga cttctggtta
    80641 tccacggtag tgcagtaaaa ctggttgtac cagagagtat atctacgtct gatagctcat
    80701 tacaggaact atcgcccata tataatgaat tggtaattgt taagggtgat cttgttactg
    80761 tgaagttaat taaaaaatct attgtaatag agttgattgg caaagcgctt caaagtggca
    80821 aaacaggaga tgaaattaag atttcattac aaagcggcaa aacagttaca ggcaaaattg
    80881 tcaataatgg tgttgagtgt tatctatgaa aaaattttta ctggcgatag ctcttattat
    80941 gatatctgct gtttatgcag atactatctg gaaagataaa aatatctata cagcgcagga
    81001 tacaatcaaa gttggtgata tttttactgt agctataacc gatatttcgc agatgcgttt
    81061 tactctgact cttgccaata atgatgcgtt tactgtaact tcaactcccg atgggaatat
    81121 tacaccattt cttcctcagg ttaatgcaaa taaaaaagct acaaaaaata acagcacaaa
    81181 tcttcagcac cgtggaaaca ttacacttca tattgcagca actgtacagc agcggcttga
    81241 aaatggaaat ttcaggattg caggcacaaa aacattttca ttcagtggcg taactatagt
    81301 gtttaatgtt agtggtattg ttaatcctgc atcaataaaa ggcggtacta tcaattcaag
    81361 tgatatagca aatttagttt tacagataca gggtgtaaag gaaggcgtaa gaattacacg
    81421 agaaccactt aaagaaggtg aaaccgcttc accagcattg accgaacagg aaaagcagca
    81481 gatcattatc caatatttgc aaagaatttt gggtgagtta acacagcaat gatgaatact
    81541 gtatataaaa taattatctg tataatgaca gttacatttt ttactgtaaa tctatgtgca
    81601 gaggttcctg taaaaatcaa agatctggtt gccatagatg gattaaaaga aaaccaggta
    81661 tatggatatg gacttgttat tggtttacag ggtactggtg atagcaaagt taaattaaca
    81721 cagagttctc tttccaatgt attaaaaaac ttagggctta atgacgaaaa tattaattct
    81781 aaaaatatag cagcagtcct ggtaactgca actctgccac cgtatgtaag aattggtgac
    81841 aggattgacg ttactgtttc atccataggt gatgcaaaat cactggaaaa tggtgtttta
    81901 ctacaatcag cattaaaagg tgctgatgat actacatatg ttgtggcaca gggtgttgtt
    81961 ttgattgctg gtgacaagcg taaaggggca accataggca ggatagtttc tggtggtata
    82021 gttgaaaaag aaattctgcc tgatatagtt aaagacggca atattaagtt ttcacttctt
    82081 aattttgatt tttcggtagc aaaagaaata aaaacagcta ttgaaaaaaa gtatcctgaa
    82141 tcaaagccca taatagataa gggatatatt caatgtactc ttccacaaaa tgttactgtt
    82201 actgatttta tagcagatat agaaaacatt gagatagtac ctgtatatag tgcacgagtg
    82261 gtaattaatg aacgaagtgg taccattgtg atgggtggtg atattcgtgt ttctcaggca
    82321 gtaataagca agcaaggggt aacaataact atcgaagaaa cacgtaaaaa aggtagcatg
    82381 tatttattaa aagatgctgc aactgtcaaa gatttagttg attcattgaa ttatattggt
    82441 atgagtactc aggatattat cgtaatttta aaggcaatga aacaggcagg atgtctgcat
    82501 gcagagttga tagttcagtg aggtttgtat ggatacaaca attaaaaata ttatcgacag
    82561 ccagaaaacc gtacagtcta ttaataaaca aaaagatata gaacaaaaac tacacaatac
    82621 ctctacagat ttcaaatcca tgcttaataa tgcaatagct aaaaacaatg ataaacctgt
    82681 tgataaaaag cttatggacg tttgcattga aatggaatca ctatttgtat atcagatgct
    82741 caaagaaatg cgcaaaacag ttcacaagga aaatgattta ttatatggtg gaatggcaca
    82801 ggaaatattt gaagatatgc tgtacaatga atatgcactc cagatgtcaa aaacagcaaa
    82861 ttttggactt gctaaaacac tctacgaaca gttaagccag aagtaatata aataaatctc
    82921 atcaaataaa ccagtaacat tcactataac taattgacaa aatataatta tcaattacat
    82981 tcgtttaaac tttttacata agggttatct atgcacttat ttgagctttt tataggctgg
    83041 cgatatctca aagcaaaaaa atctcaaggg tttatttcat tcaatacatt cctttccata
    83101 tttattgtcg ctattggtgt ttttgtcctc attgttgtga tgtccgtcat gactggattt
    83161 cagagtcaaa ttcgcgacaa aattttagat gttgacgcac atatcacaat aagccgttac
    83221 tatgctgata ctgattctga aggaatagcc aattacaggg aactatcgga caaaattaaa
    83281 aatgtaaagc agataaaaac aattatgccg tttgttcagg ggcagggtct tgttcgtgtt
    83341 aaatcatata ttttccctgt tgcaattcgt ggttacggca atccaggcga agtccctaaa
    83401 gacattacaa aatttataac tgcaggcgat aaagatttta aaaatgctac ttcaatagca
    83461 attggcgagg agatggcttt caattacaat ctcaggcttg gcgattacat tgaaataatt
    83521 gtccctaaag ggcgtttgac tgcacgggaa ggaattactc ctggtattgg ccgctacaga
    83581 attgcttcct tctttaaaac cgggtattat gattttgaca ccaagcttat tcttttaacc
    83641 atcccacaaa cgcaaagatt atttggtata ggtaatattg tgagcggttt agctttaaaa
    83701 ctttttgatg tatatgatat ggacaggacc gcttcgttac tacagagcat aatagggtat
    83761 gactaccagg taataactgc tgaacagcaa aatcaaaatt tattttatgc actccgttta
    83821 gaaaaattaa tcatgacaat cattctattt ttagtaataa tatcagcttc atttaccatt
    83881 atgggcacgc ttgtcatggt ggttatggaa aaacggaaag ctattggcat tttaaaagca
    83941 atgggagcac agccgtattc aataatgatc atctttatcc ttgagggttt ttttataggt
    84001 cttattggtg ccatcattgg agttattact ggccttgcaa cggcaataaa tttagaggct
    84061 attattcact gggttgagcg ggttatcaat agcataatgt cctggatata tataacattt
    84121 gatctgggtg tattttatcc agtgtcactg gtaccaaaaa atgtgtatta cattgattcg
    84181 ttgccaactc aaattcaacc cgagtttgtc atgcttgttg ctatactggc aatttttatt
    84241 gccacagttg cggctatttt accttcatgg cacgcatcca gactgcatcc tgttgaatct
    84301 atacgatatg aataaatcta atggattata gtatgaccca gagcaataat tcagataaca
    84361 ctatcataaa aacagaaaac ctttttaaac aatatggata tggcacaaat tccataacag
    84421 tactaaacaa tatcagtata agtattaata aaggggagat agtttctgtt atggggcctt
    84481 caggtgcagg caaatccaca ctattgcata ttattggttg ccttgacaca tttcaaggtg
    84541 gcagagttca gatattgcaa caggatattt ctccattttc atatgatgaa ttatctggtt
    84601 tcagaaatca gcatattggt tttatatttc agatgcataa tcttatgccc gagtttacag
    84661 cattagagaa taccatgatg cctttattaa ttaaacgcca tgcctattca aaagctaaac
    84721 agaaagcatt tgaactttta gaacattttg gtgtggcaga tagggccaac cacaaacctg
    84781 ctgagttatc cggaggtgaa tgccagcgca ttgctgtagc acgagcactt ataacagatc
    84841 ctgatattat attagctgac gaaccaactg gcaaccttga cagaaaaaat tctgatattt
    84901 taattgaact tcttttaaaa agttgtgtag agaagggttc cacagttgtt attgttactc
    84961 atgaccagta tattgcttcc aaaactcaaa gggttattac gcttattgat ggtatgatac
    85021 aaaaagatat gagtaattaa atgtaaggat gttattatga agattgaatt tgttggtgga
    85081 gcacgaacag taacaggttc atcgtttata gttaaaaata acaattttac tgttatggtt
    85141 gattgtggaa tgtttcaggg cacaagggaa ctgcgtgaga gaaattacct caatctgatc
    85201 tatgcacctc agcaaattga ttgtttgctt ttaacacatg cacatattga tcatagcggt
    85261 ttaattccaa aattagttaa agaaggcttt gcaaaaccaa tttatgcaac caaagccacc
    85321 tgcgaattgg caaccataat gcttcctgat agtgcccata tacaggaaca ggatgctgag
    85381 attataaaca aaaaaagaaa aaaaataggg cgtgaattca ttcaacctat ttatacttct
    85441 gaagatgcag aacggtgttt gcaatatttt aatccagttc attaccatga aactgtttcg
    85501 gttcatcctc aaattacagc acgattttat gatgcaggac atatactggg atcatctttt
    85561 attgaaatgc atgtacagga aaacaataaa gaacaggtca ttattttttc tggtgacatt
    85621 ggacaaaaaa atcaggcaat aatcagagat ccagagttac ctgaatatgc tgatatacta
    85681 cttattgaat caacatacgg tgacagatta cataaaagta aagaagacac atacaatgaa
    85741 ttaaaacatg tcatacttga ttcatataat aaaaaaggca acatcatcat tccagcattt
    85801 gctattgaac gaacacagga aatattgtat actatagctc agttggtaaa aaaaggtgaa
    85861 ataccgccaa taccagtata tttagattca ccacttgcta catccgctac tgagattttt
    85921 atgaataatc aggattgctt tgatgaagca atgttaaaac tccttaaaac tggtgatagt
    85981 ccacttgatt ttcccaatct tcattttgta caaacagttg aagaatcaaa atggttaaat
    86041 accaatgcta aaggtgcaat aattattgct gcaagtggta tgtgtacggc tggaagggtt
    86101 aaacatcatc ttttgaataa tctttataaa cctgaatcct cagtagtatt tgtagggtat
    86161 caggctgaag gtacattagg ccgacgtata attgatggtg ctaagcttgt aaaaatttat
    86221 ggtgaaaatg ttgccgtcag agcaaaaata tacactattg gcggattttc cgcacatgcc
    86281 gataaggaag gtttacttga atggatgagc catataaaaa atccaaatct taaagttttt
    86341 gtgatacatg gagaagaaaa agcatcaaca gcatttgctg atgaaatccg taaccgcttt
    86401 gggtactccg tctatgtgcc tcactggggt gaaataatag actttgatac catgaaatct
    86461 gaatatgctc aatacagtcc cattgaaaaa acagataata ttgaatccga aattgaaaga
    86521 atttcactgc ttctttctac cataaaaatg cggtatcaga aagctaaaga agagggtagg
    86581 agaattaatt gggcacaggt tgaaaatgat atttcagatg tacgagaact tgttacaatg
    86641 atcaatgacg aattgtaatg ttttgtaagt gtacactgcc tattaacaat gtgggcagtg
    86701 cacactcagt gaaattattc atatactttt tcttcaacct gatcagcacc gggagcatcc
    86761 tgctcaacat cacgaatatt ttcttttgat attactttga agtactcctt ataccattcg
    86821 ttctgtataa taagatccat aatttcattg gtgccaaccc atatcatcga aagtcttata
    86881 tcacgcacaa ttctttcaat tggatataca ttggtatacc caataccacc cataacctgc
    86941 atgcaattgt tggcaatagt ccatgctgat tctgttacaa acttttttga ctcggatacc
    87001 atgcgtctga ttcttcccgg atgcacttta cctgaatcaa tagctaacgc tgtactatat
    87061 actaacgatc gcgctgcatc tagtaacatc acgctatcag caactttaaa tccaactgct
    87121 tcaaaattag ctatgggctg gccaaatgcc ttgcgcattg atgtatagcg tgtagctatt
    87181 tctattgcag ggcgaacact acctattgtc atagcagctg tccctaatcg ttcagggatc
    87241 atatttttca caaaaacatc aaaaccacca ttaataccat ttaatgcata tcgctctggt
    87301 accctgactt catttaaaat taaacgtcca gctcctccac ctcgtactcc catcaatccg
    87361 taaatatatt tagtctcaac accctttgtt cttggcacca taaaacatgt cagccgttca
    87421 tgaggcttcc cctcaggatt tgtcactgca tataccataa accagtcagc accttcagca
    87481 ccaactatga atcgcttttg acctgtaata atccagtcac tgccatcttt tttggcgata
    87541 gttgttgcac caaaaaagtc agacccgcca cgcggttcag tcaaacattc tgctgcatac
    87601 acatcacctt tgagtaaagg tacaaccact tcctgtttta attcctcatt tccaaattca
    87661 actatagcct cacatacaat gtcagcacca acaccccaca aacacgccag tgaatagctt
    87721 gcaacaccaa tttcttcagc aacaatgcaa tcatccaccc atttaaggcc tctgccacca
    87781 tacttttttg gcaatcgtat tcccaacaga tttcttttac ctgcctcttt caagaattct
    87841 ttagggaagt gaattttttc agcgtccata tcaagaatca tttcccgtgg
//
LOCUS       DAORYL010000005        66261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_364_length_66261_cov_12.385358, whole genome shotgun sequence.
ACCESSION   DAORYL010000005 DAORYL010000000
VERSION     DAORYL010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 66261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 66261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66261
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_364_length_66261_cov_12.385358"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLV81_02130"
     CDS             <1..234
                     /locus_tag="PLV81_02130"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366725.1"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HOM08932.1"
                     /translation="SEVEEGRDFTDYINPDSLKVVNGYIEPYVQGAQVYTNFQFERLG
                     YFTVDPDSNNDRLIFNRTVTLKDTWAKIAQKED"
     gene            304..376
                     /locus_tag="PLV81_02135"
     tRNA            304..376
                     /locus_tag="PLV81_02135"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:337..339,aa:Gln,seq:ctg)
     gene            459..1703
                     /locus_tag="PLV81_02140"
     CDS             459..1703
                     /locus_tag="PLV81_02140"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HOM08933.1"
                     /translation="MYSIALIIFIITYSGIIFTRLPWVNVDRPSAAYFGAIAMILTGV
                     LSFEEAIRAIDFNTIALLLGMMIVIATLELDGFFAFIAQKTISFSRTPLQLLWIITFT
                     TGIASAFLVNDAVVLMFTPIIISICRSAQLDPKPYLIAEILASNVGSAMTITGNPQNM
                     LIGIYSGIPYVYFLLHLLPVSIIGMIIIVVCIKRMYSHTFETQQVLQYSQNDYKYNLS
                     SMIFSVPIFILITLFFLLHHVLDISIPLIALTGSSLILLLGKIKPSKIIKEIDWVLLL
                     FFASLFIVVKGAEKAGILSLLLKFSHFNNSLHSITLLHAVSLVFSQIVSNVPFVILLG
                     PTLKYSNSNIIWLALASSSTLAGNATIIGAMANLIVLESAAKSGIQISFMEFLKPGII
                     VTVLTLMLSIAVLYFYSVANSL"
     gene            1849..2190
                     /locus_tag="PLV81_02145"
     CDS             1849..2190
                     /locus_tag="PLV81_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOM08934.1"
                     /translation="MQIDVHKEGPLLIFTLNGRYDISEVHNFETVYRHHIEDNPKIIA
                     LNLRDLKYIDSSGIGSLVRCMNLALKSNIEFVCYDINENVESIFKIAKLDQFISILTQ
                     EEFIKKYIKKQ"
     gene            complement(2177..3169)
                     /locus_tag="PLV81_02150"
     CDS             complement(2177..3169)
                     /locus_tag="PLV81_02150"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOM08935.1"
                     /translation="MGVANLFTYKYLKRKKLTVIIYLIILCAIVTINVLFLLAHQFAL
                     NYIAISFFSEIMLLILSNIVAVSISFVIVNKMLQKRYNDVAASLLTVLKQFEKGNFTY
                     PFSIDNGRFDNVLRYMQKLMMRQNSTPASFDEKLNERVFEIITVLISAMEDKDPYTSG
                     HTTRVAHYALAIGKRLGLSQEKMRLLNHAAAAHDIGKIGIPIEIIEKNGRLNEKEKLM
                     MESHPIRGMKILSSIDYLEDIASIIMYHHERADGKGYYQLPGEKVPVEAKIIAVADAL
                     DAMLSDRPYRKALSLYEAKQEILKNKGTQFDPEVVDALINVLEEKEPLKEFIAF"
     gene            3358..4332
                     /gene="trpS"
                     /locus_tag="PLV81_02155"
     CDS             3358..4332
                     /gene="trpS"
                     /locus_tag="PLV81_02155"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006718952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOM08936.1"
                     /translation="MSRILSGIQPSGTLHIGNYFAMMKPMIELQHNNDLFCFIVNYHA
                     LTSIHNPQELSENTINAAMDFIALGLDPDKVHFWVQSDIPEVTELAWILSCHTSLGLL
                     ERSHSYKDKIAKGIVPNTGLFTYPVLMAADILLFNAEVIPVGKDQKQHLEITRDIAER
                     FNQMYGEIFVLPEPMISEDIAVIPGIDGQKMSKSYNNTINIFEDEATLKKKVMRIVTD
                     STPVEAPKNPDTCNLFALYKLFAEPAKTEILKERYRKGGIGYGEVKKELFGMIWEYFA
                     PFRDKRKQLQNNKSEIAAILKKGAEKTRPIAQETLLRVKKAVGLDYLQ"
     gene            complement(4333..5730)
                     /locus_tag="PLV81_02160"
     CDS             complement(4333..5730)
                     /locus_tag="PLV81_02160"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOM08937.1"
                     /translation="MKLRIVLITVITFIICTAPLTDAKTVKKAQGSENISDLSKFNEQ
                     LVKVTNSAIPTVVSISAEKKAGSPRESKRMPMPFPVPKEAKALGSGVIIDKRGYIVTN
                     NHVVKNTKSITITLFDKRTFPCELVGSDPATDIAVIKISGTVPQDLPVAVMADSDTLK
                     VGEIAIAIGNPFGFGHTVTMGIISATGREGFGLADYEYFIQTDAAINPGNSGGALINI
                     NGKLIGINTAIFSRNGGYMGIGFAIPSNMVKQVVDELINKGKVTRGWLGVYIQNITPE
                     IAKNFKFEGRKGVLVADIMKDSPAKNAKIEIGDIIISVNGVEVSDINQLRRYVASLKP
                     GSTAKVKIFRGGQELEREVLIAELPAQAQAPEEKMEEVDTIGMRVGDITEENAYKFRI
                     TDTTGVIVLEIQEQSPAYEAGMMVGDIIKEIENTPVENVDKYNELLKQYEKKETLLLL
                     VKRGGIHKFVVIKKQ"
     gene            6130..7149
                     /locus_tag="PLV81_02165"
     CDS             6130..7149
                     /locus_tag="PLV81_02165"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOM08938.1"
                     /translation="MKHKYTLILTIIVVSLLLFSCSRQQRYSLSKMAMGTIVTITADS
                     TSNKKAYEAINQAFETIKHIEEIMSPYIPTSDVTHINNNLQTNVSNDTFTVIKLALEL
                     SHNTHGLFDITFASVGSLWQLQSPSFVPPSQGSINQRLNCVGYRYIHLNPKTRTVIKQ
                     KPCVTIGLGGIAKGYAIAQAALVLKQCGIQQGIVEAGGDLQVLGDNYGYGYTIGIKHP
                     RQEGIIGTVILHDGQAIATSGDYERYTIYKGKRYHHILDPATGYPAQSDIISVSVITS
                     NPTICDGYATALFIMGTQQAIAFCKNNAAFNLYCIIIDKNLNIYCSKDLLQRVSFPHH
                     SVYPF"
     gene            7492..8538
                     /locus_tag="PLV81_02170"
     CDS             7492..8538
                     /locus_tag="PLV81_02170"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOM08939.1"
                     /translation="MPYKIALYPGDGIGPEVVAEAAKVIDACNIDVHYTTFNWNTTLY
                     NKTGKCAPENYLELLSSFDAILLGALGNTHNAPDHIAVEPLLTIRKAFDQYVNIRPAM
                     LFPGVESPLKNKKPYDIDMIVIRENTEGEYTALGGRHYIGTPDEVAMQINYFSRKGTE
                     RIIRYAFETARNRKRKTLTSITKSNALKYSMVFWDTVFYEVAKEYPDVIASSMLVDAA
                     AMNMVRCPEQFDVVVASNLFGDILTDLAAIVVGGMGFAASGNINPTRKYPSMFEPVHG
                     SAPDIAGKGVANPVAAILSGALMLEFLGEHDAAQKIKSAVVNHLNDGTVKTPDRGGNA
                     TTADVGTDIAQRIQ"
     gene            8610..9494
                     /locus_tag="PLV81_02175"
     CDS             8610..9494
                     /locus_tag="PLV81_02175"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOM08940.1"
                     /translation="MALTEYTIPSTKINDFFISVSDGMELYCIDFIPKKDSSSKPIIF
                     FVAGWISHITGWKDVLLTLTDEYRVIYIETREKTSSRIPEGKTYKDFSFNVMRMVLDL
                     HEVIQKVIPKNRTFVMAGSSLGSSVILEYLAMHTRKPECALLIGPLPEFRFPPLLGDI
                     VPRFPASWYNVLKELVKWYLRNFRLDTEKEKEQMAKYTNTINSADPYKLKPNALALKN
                     YNLWDKLPFITTPCYIFGAKTDKLHATDDIKRLLQNLQYAEYIECESNKETHSRVVAI
                     HFKNIISKKLYTKIKPKK"
     gene            9562..10050
                     /locus_tag="PLV81_02180"
     CDS             9562..10050
                     /locus_tag="PLV81_02180"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOM08941.1"
                     /translation="MSFCKVEIATIVLDEESNTPIVVLQDNETGNILPIMIAPLEASL
                     IAIELEGKKPVRPLTHDLIITMLKTFQYTLTSVIIDDLKDNIYFAKLNLIAPDNSEII
                     IDCRPSDAMAIALRAHVPIYVKKKVFAIAQGLENLSQIDKETMKEVLEDIDIDDVGGK
                     IM"
     gene            10074..11918
                     /locus_tag="PLV81_02185"
     CDS             10074..11918
                     /locus_tag="PLV81_02185"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HOM08942.1"
                     /translation="MDAEKNIAALHNIIRDLTEIESRLNEYIDTTMWVSDPHGAGDRF
                     VSILKGRFGLVWRICYEALPKTFSKEKIDYLGRIIRKERYFEDEVYRLDRQDIISSLI
                     RIVQYRVQNVRDFDEIRNNINKDLKHVLENLILNYPVPNMIYENELIADKIISSLCKI
                     VKQVILGHLIVLGDVFDRGDEPDKIIRILNQKDIKRYLTFIWGNHDILWMGAAAGNKS
                     LIAEALRISTRYDNLAFLDRIGINITKLKEFALHTYTGEIPGNFKAKQDISRRMEKAL
                     AIIQFKLEEQTIRENPEFNMESRLWLHKLAEMLKNNDTSGLTDTQFPTIDLDNPDKLS
                     PEEEEIINDLTYQFTTSKKVRYLMEFLFEHGKLYHIHNYILNIHALIPSTHDGQFEEF
                     LGYRGKALLDHLQHRIKTIGKNYLEGKPQNPKDLALMFYLWCGSKSPFFGKNAMKTFE
                     RYFIADKSTHKEKLLYWGENLLKKEFMDKIMEEFGAERIVYGHTPVDVRKGEKIASPD
                     GRAINIDGGFSDAYLSRGHALIQTPYSLYAIILPTPEETIQARQKKEPVNLMVEIIDN
                     FETPLRVKDTYTGKLLIKKRNELYEKLLQYSEHYSDMEHNTTLNYSIV"
     gene            complement(11970..12368)
                     /gene="atpC"
                     /locus_tag="PLV81_02190"
     CDS             complement(11970..12368)
                     /gene="atpC"
                     /locus_tag="PLV81_02190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOM08943.1"
                     /translation="MAMKLNCNVLTPERQIYEGQVDFAVVQAYDGEMGFLYNHAPLIS
                     ELGTGEIRLRSGDSTEYFYVDGGFVEIKNNSLIILAQEAVSKQDLKPEVLESKIKEIA
                     ALPKPETFADRLNIDIELKKLKARLKIAKR"
     gene            complement(12390..13802)
                     /gene="atpD"
                     /locus_tag="PLV81_02195"
     CDS             complement(12390..13802)
                     /gene="atpD"
                     /locus_tag="PLV81_02195"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOM08944.1"
                     /translation="MAENIGKVVQVIGSTLDAEFQEDKLPAIYNALKLEVEVMGEKKT
                     LVCEVAQHLGGNRVRAVALGSTDGVKRGTPIIDTGSPITVPVGEETLGRVFNLLGDVV
                     DKGAPITVKERRSIHQEPPKFDQLEPKAEIFETGIKVIDLIEPYIKGGKTGLFGGAGV
                     GKTVVIMELINNVAMQHGGYSVFAGVGERTREGNDLWLEMKESGVINKACLVYGQMNE
                     PPGARLRVALSALTMCEYFRDLSGRDVLLFIDNIFRFSQAGSEVSALLGRMPSAVGYQ
                     PTLATEMGELQERITSTKRGSITSVQAIYVPADDLTDPAPATAFIHLDASTVLSRQIA
                     EKGIYPAVDPLESSSRILSPEIVGEEHYAVAMEVKRILQRYKDLQDIIAILGMDELSE
                     EDKLLVQRARKIERFLSQPFFVAEQFTGMPGKYVKLEDTIRSFKGLINGEYDHLPEQA
                     FYMVGTIEEAVEKAKKLAGN"
     gene            complement(13850..14734)
                     /gene="atpG"
                     /locus_tag="PLV81_02200"
     CDS             complement(13850..14734)
                     /gene="atpG"
                     /locus_tag="PLV81_02200"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOM08945.1"
                     /translation="MATQREILKRIKSVKNTQKITKTMEMVSTAKMKKMQDRITMSKP
                     YGEKLETIIDHLRQTGVEDVYDPLMQERPDPNRILVLMITGNRGLCGGFNTRVIDNTI
                     NFKNKLTIEEGKEVLIYSMGKKGSNYFKFIKEPIYKSVLNPEDKFKFEDASKLGEELI
                     NLFVTGEVDEVYVSYTKIMTRSTQKATITRLLPLKPESVAIQESVEDFHVEYIFEPNP
                     YKIFSSLLPLYIKMKLYLAVLESGFSENFARRVAMKNATDAAKEMVRDLTITYNRARQ
                     AKITKEISEIVGGAAALE"
     gene            complement(14760..16289)
                     /gene="atpA"
                     /locus_tag="PLV81_02205"
     CDS             complement(14760..16289)
                     /gene="atpA"
                     /locus_tag="PLV81_02205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOM08946.1"
                     /translation="MKIKTEEIKSIIKKEIEGYKSQLDVREVGTVIQVGDGIARIYGL
                     DNAMAGEMLEFENGAYGLVFNLEENSIGAVILGDYLSIQEGDSVKRLNRVLAVPVGEE
                     LLGRVVNPLGIPIDNKGPINSSKMRPVEFIAPGIADRQPVKEPLQTGIKAIDSMTPIG
                     RGQRELIIGDRKTGKTAIAIDAIINQKGKGVYCIYVAIGQKASTVAAVVEKLEQYGAM
                     DYTIVVAANASDPAPLQYIAPYAGCAMGEYFMYEKNGHSLCIYDDLSKQANAYRQLSL
                     LLRRPPGREAYPGDIFYCHSRLLERAAKLSDDLGGGSLTALPVIETQEGEVSAYIPTN
                     VISITDGQIYLVPGLFASGIRPAVDVGISVSRVGGNAQIKAMKKYAGSLRLDLAQFRE
                     LEAFAQLGTELDPATQRQLDRGQRLVEVLKQDQYEPMPVAEQVAIIYAGTQGYLDKIP
                     VSEVRKFEKEFLKFMREAHPELLDGISSTGELKDPDRFGKVVGDFVESYLAGKKADVR
                     A"
     gene            complement(16279..16848)
                     /gene="atpH"
                     /locus_tag="PLV81_02210"
     CDS             complement(16279..16848)
                     /gene="atpH"
                     /locus_tag="PLV81_02210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOM08947.1"
                     /translation="MATSEVAKVYAGALLEIAHEKNAISKVEEELSFLAELLKNDYDL
                     MRYFSAPGITKESKKQFVDKVFGSELSELMVNFLKVLIDNGRQQDIPDISVAFSELLD
                     IVNNRQKIKLVSSVKLDSDIINRIKKSLEDRFNKNIIVEEVIDPTILGGIIVQVDDLI
                     IDASLKNDLFKLREKLLQSKIRGEVAYED"
     gene            complement(16890..17399)
                     /gene="atpF"
                     /locus_tag="PLV81_02215"
     CDS             complement(16890..17399)
                     /gene="atpF"
                     /locus_tag="PLV81_02215"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOM08948.1"
                     /translation="MEVLKEGLLKVDPGLLLWTIITFIVLLLILWKAAWKPIVEALDA
                     RAEKIRGDIESAEKSRLEAERLFAEHKAMMDKAKEEAASIIAEGKADAEKLKNSIVEK
                     ANQEAKDLIERARREINLAKDKALAEIQAEVVTISTDIAAKIIAKNLKVEDQKALVEE
                     ALQKIRTVQ"
     gene            complement(17422..17703)
                     /gene="atpE"
                     /locus_tag="PLV81_02220"
     CDS             complement(17422..17703)
                     /gene="atpE"
                     /locus_tag="PLV81_02220"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOM08949.1"
                     /translation="MEYLGLSYLAAGFGAGLIVFGAALGIGKLATGALEGMARQPELS
                     GDLRTAMIIAAALIEGFTFFALVVTFMLATKAPIATPVEKPAAEQTEVK"
     gene            complement(17797..18654)
                     /gene="atpB"
                     /locus_tag="PLV81_02225"
     CDS             complement(17797..18654)
                     /gene="atpB"
                     /locus_tag="PLV81_02225"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOM08950.1"
                     /translation="MVQLLQGLQAIIQSSKSGEEESMYDIVMHHLTDHPIHDGFIGKI
                     NEKFFHEKLFGIFDMTITKWVLMLWLSAFLCLLVFIPVARKIKNSSYGSRSRWVNLWE
                     VLIAFVHDEVVEPNFDHHYVKKAMPYFLTVFFFILFCNLLGLIPGMSTATGNLAVTGG
                     LALCTLVGMLGIGMIKQGIFGYWAGLVPHGVPAFVLPLMFPIEIIGLLIKPFALTVRL
                     FANMTAGHVVIIIFIYLVMMFQSYWVGIGSVAGSLMIYLLELLVAFIQAYIFAVLSAM
                     FIGASMHAH"
     gene            complement(18709..18981)
                     /locus_tag="PLV81_02230"
     CDS             complement(18709..18981)
                     /locus_tag="PLV81_02230"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOM08951.1"
                     /translation="MKRDITRIILAYSTIGIQLAVTILVFLYGGFLLDKHFNSSPIFV
                     VIGTIIGMGAGFYNLMKSLSELDRLLKKSQLGDNNNSSGSKRRKWM"
     gene            19318..19569
                     /locus_tag="PLV81_02235"
     CDS             19318..19569
                     /locus_tag="PLV81_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08952.1"
                     /translation="MKLSVKLIEKNNEFIATCPELDISCYASNKIEAIKRIQSILYFY
                     MQSAQELGFSVENFETIDVDGKHYLFNYDIYPPLNSTMH"
     gene            complement(19623..21008)
                     /locus_tag="PLV81_02240"
     CDS             complement(19623..21008)
                     /locus_tag="PLV81_02240"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOM08953.1"
                     /translation="MKRLLLVASIIGIIIGFIGCKCSSDVLATYDGVEITRGQFKEWL
                     EDKQFSVEAILKSKKQQKDKLEMMAIEHIALQEAKKENLIETEKYKFLEDMAYESILL
                     KHLYNKKIKDVVEFNEPAYHVKQILIRVRDFKIVNNKRVNLSTSELEKAYSEAENKAK
                     EIIDKIKSGQKFEELAKTYSDDFSKKNGGDIGYVVKDMLAPEVASAIESSKSGIIENP
                     IKTQQGIYIIQLMDKQEITKKNLNKIIKDEMQAKRLEQILLRRASNNYIEKLMSASDV
                     QFNEKNCKSKNPKDVIFKVGDSSFTVADLEKRISYFQPKGSSLSKATIDDTKKIDIAK
                     NAFKVALLKREAIKENLQTNEELQKEFQKRKNMLFAKEYMDYIGERGIAVTPKEIRDE
                     YEKYKDTRFTTFVNQKGKQVKQILPFEKVKDQIESMLYRKKRSENLAKWKRDMLHINK
                     FTIHEDELEGK"
     gene            21387..21920
                     /locus_tag="PLV81_02245"
     CDS             21387..21920
                     /locus_tag="PLV81_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08954.1"
                     /translation="MRDKAFYRNKLYTLLHQDHSFDCETLIEYGYDAIEKGNFRKAFR
                     LFALGYRLIGNHPEILNGLGLTLCEMGKLKFSKNFLLFALKKFPENVFILSNLATVYF
                     EEYDYDKAIYFYTRAINVDPFFIEAHINLINVYFERGDIFMAYISCLDLLKKDPDNYE
                     VQELCNDIILNMGLSLV"
     gene            complement(21923..22342)
                     /locus_tag="PLV81_02250"
     CDS             complement(21923..22342)
                     /locus_tag="PLV81_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08955.1"
                     /translation="MHKITTYTNLLSVILLIVIVSWFYSCTKEPVDDAAMIAIRYLTT
                     TDEKQLLTYLNNASQRKIENMKKTYGSVYPFIWVHHDTDATWEVVKVEKTDNNATVTL
                     QCIKHKKQNAIGLEVVHTLVKEDGKWKIDISDELPSR"
     gene            complement(22459..23658)
                     /locus_tag="PLV81_02255"
     CDS             complement(22459..23658)
                     /locus_tag="PLV81_02255"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOM08956.1"
                     /translation="MKLRPAYKKIGMLFSGGPAPSANTVISSVALNFLDNGIPVIGFY
                     RGYEFLQDFNINYPKIKKGVHYEELDYNITHVRNERGIYLKTSRANPGKDIKALDDLQ
                     NAEKNKKLKNILDAFEYLKIGALISIGGDDTLKTANFLNLMGYPVIHIPKTIDNDYYG
                     IPWTFGYWTAVDTAQKMMLNLKADAQATDSFFIVELMGRKAGWITYAAGIAAEAIMMV
                     AAEDIDEDVLDIEVLVEKITNVILARENNRRPYGVIAVSEGIVDKLPDEYKPKHTDKH
                     GNILYREAKIGEILAEHVKKRYQEKTGMEVKIVAKQIGYETRSAPPISFDVVLGAMLG
                     YGAYKFYSENMFGVMVSVTDNFDLKAVPFSELIDPDTLKTRLRDVPKGSDFYTLKERL
                     SYRKSWE"
     gene            complement(23745..24419)
                     /locus_tag="PLV81_02260"
     CDS             complement(23745..24419)
                     /locus_tag="PLV81_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOM08957.1"
                     /translation="MREILERAIANKQKALSEFDSKSVIKIAGIPVSRNYLAKSREEA
                     IAFAQELGFPVVLKGCSDTLTHKTELGMVKLKLKTEEEVARAYDEITGKGVPLDGVLV
                     QEWLDGDNREFVLGTIRDPQFGPCVMFGLGGVFTEALQDVSFRVAPITELDAYEMMDE
                     LRTKKLLGPFRGSPAVDRESLAKALVGLSELVSKYEEIAEVDINPVIIDGNKPVAVDA
                     LIVLKS"
     gene            complement(24412..25878)
                     /locus_tag="PLV81_02265"
     CDS             complement(24412..25878)
                     /locus_tag="PLV81_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOM08958.1"
                     /translation="MLDSLFNPKSVAVVGASEIPFKWGTYISSNILDGKFKGRYYPVN
                     PNILEVFGFKTYPSIRELPEAVDLVFITTPAKTVMQILQDCITKGIKNIVMITSGFSE
                     TGAEGTQMEKEIISLAKNHGLNIVGPNTMGIVNTHCSLYATGAITRPKPGGISIIAQS
                     GNLGYQIMEWAESEDIGIAKFVGSGNEAVLKIEDYLKYFKTDSQTKVILMYVEGVDDG
                     RVFVEIAKETSLHKPIIVLKAGRTEIGSKAAASHTGAMAGSFSIFKGVIQQTGIVLAE
                     SPRELLTLSAAFDSFPLPKGNRIGIITLGGGWGVVTADECAERGLVIPHLPQSIKEEL
                     DKRLPPFWSRNNPVDLVGQPDFQLFKDAVELMVAHEDFDAVIVLGIVGSKVFAYRAAE
                     AVYNLGKMEKSVFDDFKKLLYIHQKEFLDRLIELMNRYKKPILPVSLSKMPGDETIHS
                     DSGNYKVVIYDSPEEAVLCLSKMYQYYNYVEKRSRVYA"
     gene            complement(25981..26967)
                     /gene="galE"
                     /locus_tag="PLV81_02270"
     CDS             complement(25981..26967)
                     /gene="galE"
                     /locus_tag="PLV81_02270"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOM08959.1"
                     /translation="MKNILVTGGAGYIGSHVTRLLAKKGFNPIVYDNLSGGFKDFVSG
                     FEFIKGDIGDFDKLAHVLSHYNIICVMNFASFIAVGESVQYPLKYYENNVAKTLTLFE
                     AMIKTNVKRFIFSSSAAVYGNPEIIPIPEESPMKPESPYGKTKYFIEEVLRDLSVSNS
                     FKAISLRYFNAAGADESGQIGESHVPETHLIPLVIRAVLDPEYTVTIFGTDYNTPDGT
                     CIRDYIHVNDLGDAHILALENLLESGKSDVFNLGNGKGFSVREVIDSVRRVTGKDFLV
                     KEGARRPGDPAVLVASSQKAKELLHWQPVYTDIDSIVATAWKWESQRKRKGY"
     gene            complement(27024..28043)
                     /locus_tag="PLV81_02275"
     CDS             complement(27024..28043)
                     /locus_tag="PLV81_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HOM08960.1"
                     /translation="MHSFIAIIKEASFVSVFVIVMMLLIEYINVVTQGKIANLKSTRL
                     KQYTIAVILGIIPGCLGGFASAAMYAHGAISLGAIVATMIATSGDEAFVMFALFPAVA
                     FALHIGLALIGIVAGYCTDLIFKKHGPVEHCCDGLVIHDEIPFSHYTPGNIISLWKNL
                     SLSRASLMSLLLVALLMVITGFFHQEEGFVKIALPVIISIAIIIVAIVPEHFLQEHLW
                     EHVIKKHLIRIFLWTLSALIVIHLLVDVLHLDALIHNAQWIVLVIAALVGIIPESGPH
                     LLFVMMFAKGMVPLSVLVTSSIVQDGHAMLPLLAQSRKDFLLIKCINLIVGLIVGSII
                     MLTGN"
     gene            28247..28651
                     /locus_tag="PLV81_02280"
     CDS             28247..28651
                     /locus_tag="PLV81_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08961.1"
                     /translation="MKLHIVKRLLLVILIAVTLITFIACEDEPVKIKLMVISTVKGFT
                     GYYIVNGDTPVPFSATEDAYGIALFEKEIEDVDYLEVSATTFDGATSIEIKVYRDNKK
                     VKSSQKTIEDPYDSYTLNFEYSLGEEEQESSQ"
     gene            28676..29131
                     /locus_tag="PLV81_02285"
     CDS             28676..29131
                     /locus_tag="PLV81_02285"
                     /inference="COORDINATES: protein motif:HMM:NF020091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOM08962.1"
                     /translation="MLNFEIHDATRELSIARALKKYEPPFKVIDNYRLIDDGFNPEAT
                     VQIEANGTFIHEASTGNGPVDALAKVLKKALIPLFPEIENVNLIDYRANILDAKRGTS
                     TDVEVTIIFTDGEEVWKVYALSENINAASFRVLLDGFEYAILKKNKKKS"
     gene            complement(29239..30393)
                     /locus_tag="PLV81_02290"
     CDS             complement(29239..30393)
                     /locus_tag="PLV81_02290"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOM08963.1"
                     /translation="MKKILSSIDQAVLLRAIEQSPASIVITDTNGIIQYVNPKFEAIT
                     GYTFDEAVGKNPRILKSGFQSLEFYQHMWHTILRGEVWDGIFHNKRKDGSFFWEHCVI
                     APVVDEKGNVTHFVAIKEDITEKKEQEEALRKSEEELRKRNEIISKDLEYAKRVQHSI
                     INTTLPQYPGIKIYTQYIPYDYVGGDFFTLKTIDENLCIFMADITGHGVPAALFSTVL
                     KYAVENLWNSRILSPDQFMFKLNQELIDILSGYFFTAFYGVITTNLKGELYLTYTRCG
                     HPYPLLLSNNTISMLYSSGTLLGVYRDIRITSNQIRLSKNDRLFMFTDGLVEIDINGE
                     KDLRFDILYESILKTKDMPLEESVQILLKDATSQNRTIDDDIAIIALEVC"
     gene            30545..31135
                     /locus_tag="PLV81_02295"
     CDS             30545..31135
                     /locus_tag="PLV81_02295"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOM08964.1"
                     /translation="MRKNNRQQILDIARKLFLEFGYNGISIRNIAKRAKLTTGAIYFH
                     FKNKKQIYSTICLEAIDTLHKLFCEGMAARKTPAQKLISTYDSYLKFYYEYHDYYNIL
                     TEYKAEYDDEPDPHKDEIQKKNVALLELMAGAFTDGVKRGDFKNLDPKMVAFFLSAVA
                     EGMLQFKKLGVMDAAGISDKNFREFMKIIVGQGIQK"
     gene            complement(31156..31965)
                     /locus_tag="PLV81_02300"
     CDS             complement(31156..31965)
                     /locus_tag="PLV81_02300"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOM08965.1"
                     /translation="MNNLQGKVTVITGGASGIGAAIALEFGLHGSSIAILDVDTIALK
                     NQVNFLQRNNIPVIGFQCDITNEKQCTVTIAKIIKQYGGIDILVNNAGITQRSLFIDT
                     NAAVYKKVMDVNFFGALYCTRAAINSIIERKGVIVVISSIAGIAPLYGRTGYSASKHA
                     LHGFFESLRAELKDDGVCIMMVCPGFTRTNLQSRALSGNGTINTLDRAIVGREASPQS
                     VAQAIYKGVIKRKRMLVLTTVGKLSFLIAKYFPRLYEIMMSKSVRKEFIKR"
     gene            complement(32020..33024)
                     /locus_tag="PLV81_02305"
     CDS             complement(32020..33024)
                     /locus_tag="PLV81_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08966.1"
                     /translation="MDDTIKQMLSGYQTALAAYKQKLGESHAKLMKAYDIYAKLCKKA
                     ELLNDAMTFYSDKEVSTSMADMSALLVELAQEKQDTSLATIPSVDQVAAAYHIAYEQL
                     PMEMKKTRSVYERIFEIEKQSQNALTFLRTMADEKIFLKLSIMQQLEQLEGKKEEAQR
                     NSNPVMVNYYEKMEAKIPNVMSIAELEYYANLESEIAIYQNWWDILLLNTSVTLLCNA
                     IAGWLLTQSEDDREEVENAYRFIAYFYGIDMDELFAVPRFKDHVVKVISKSVNNSNSM
                     ESAEALISQFKNAIQACMNGRDPVKRGPAKNQTLILWEREAPLQALEEAYKTNVYKTL
                     "
     gene            complement(33024..33632)
                     /locus_tag="PLV81_02310"
     CDS             complement(33024..33632)
                     /locus_tag="PLV81_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08967.1"
                     /translation="MNILEELKGYFETRLNECKTNQELNKHQIAAYTAYLEKLDAIHD
                     DDYGKVAEAIGEMFLIARAEQRDRYESLKRIKTLFGDREGVKAEQIMIDAVQASSNHT
                     ELYSNISRAKDDAGILIQNTHRALELIVKLITRCVEYHVSCKSRKQKRKAEVVAIWNE
                     LQQCDPEITVDKIMMHKPYRRVIPFDNERLSLILSWLGEVLQ"
     gene            complement(33733..35100)
                     /locus_tag="PLV81_02315"
     CDS             complement(33733..35100)
                     /locus_tag="PLV81_02315"
                     /inference="COORDINATES: protein motif:HMM:NF015573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HOM08968.1"
                     /translation="MKLPFTFDAMLAFGWMSIMLLLGIILRANIKAFQRFLIPSCLIG
                     GAIGLVLRSYEIIPITADILETLAFHLFNISFISIGLTYNKEKADLKRAGEVLQGSIW
                     MANVEGISFAIQAIAGGLCVMLLNSIGFNLFPAFGFFTPLGFTEGPGQALSVGKVWEN
                     LGYAHSATLGLTFAVFGFLFAFFVGVPLANWGIKKGMAVHTPKELSDEIRKGIIQPQS
                     EKEIAGELTTHSSNVDAMAFQASIMGLVYMITYAVVYKVASVLSPEMGQMVWGFFFFI
                     GLVIAFIVRLIISKMGGLYLIDPGIQRRITGFAVDYLLVATIMAIQVVVVWQYIIPIL
                     VISIAGGLLTTLFCVYYGKRLWSFKLERMLAIYGTVTGTVSSGLLLLRIVDPEFKTTV
                     ALETGVMLMFASPYIILGMLWVSAPVLWQWSLIKTILVFAGMFVVAISITEIQRFLIL
                     KKKHA"
     gene            35404..36162
                     /locus_tag="PLV81_02320"
     CDS             35404..36162
                     /locus_tag="PLV81_02320"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOM08969.1"
                     /translation="MKIIDLSSTIEPSPPQTPDFLKIDVQYHSHADGAKQVEALLKVP
                     AHLMRNNEGWATETITRLGTHDSTHVDAPWHYNTTIGGKPAPTIDELPLEWFYGNGVK
                     LDMTHKPEGNPVTPEDIENELQRIKYSIKPNDIVLIQTGMDKYYGQPDYIFRGCGVTY
                     EATVMLYNFGVRVMGIDAWGWDMPLNLQAQKALEANKPGIFWAAHQADLVYSHMERLV
                     NLDKVPSTGFKVACFPLKIKRASAAPARVVAIIE"
     gene            complement(36172..36966)
                     /locus_tag="PLV81_02325"
     CDS             complement(36172..36966)
                     /locus_tag="PLV81_02325"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOM08970.1"
                     /translation="MCSSFCYHIPDGLYTIGITQNSIETLLPYLPPDIKPEYLYHSFP
                     QHTVALQQFAIAMRYVTVSEFAQFIEETGFITQAQKDGWSWTWEKGWTKKQGLWWQKP
                     FGIDELDTLYTEHSSSMCVLQVSCNDARAYCEYLSKKTGEVIMLPTEFMWEAFAAKVG
                     IPGMEQLQELSPNKHVLLSDYCHSLIELTKQRQVLPGTVWEWTDSYFQGYPGCLPHKE
                     YGTTYKVLRGGSYFSSPLQRAREYRFRRCPTARSPFYTFRICVVPA"
     gene            complement(36976..37407)
                     /locus_tag="PLV81_02330"
     CDS             complement(36976..37407)
                     /locus_tag="PLV81_02330"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOM08971.1"
                     /translation="MKNKLFIDTDVILDVALRRHPYYINSALVLSLIELKEFHGFTST
                     VIITNLYYILRKLESHDIAVEFIKKLRLIIDVLAVDDEIINLALESKFKDFEDAIQYY
                     TALKNGVKYIITRNTKDYSSGNIIACTPDEFVTIIETKHKE"
     gene            complement(37521..37769)
                     /locus_tag="PLV81_02335"
     CDS             complement(37521..37769)
                     /locus_tag="PLV81_02335"
                     /inference="COORDINATES: protein motif:HMM:NF039417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HOM08972.1"
                     /translation="MNSKLTLYIDKKVITKAKQYARKNKTSISEVVEKFLESLVDNHN
                     KYTIAPITKELSNIIVKKPKKINYKREIEDYLLFKYDL"
     gene            complement(37843..38925)
                     /gene="ispG"
                     /locus_tag="PLV81_02340"
                     /gene_synonym="gcpE"
     CDS             complement(37843..38925)
                     /gene="ispG"
                     /locus_tag="PLV81_02340"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOM08973.1"
                     /translation="MNYSRRKTIPVQAGSLTIGGDAPISLQSMTNIPVSQVKATIEQI
                     IRLQQEGAQLVRLALRTEDDAQYIKEIKKEVTVPLCADIHFNYKIALKAIENGIDKLR
                     INPGNIGAKENVVTLVKAASERNIPIRIGVNSGSVDIKKYGEVNPQTLVASAMEHVQL
                     LEDCRFTSIVVSIKSSDLLQTIEANRIFASQRQYPIHIGLTEAGYGTDCIIASSMVIG
                     TLLYEGIGDTIRLSMTGDPVHEVRAAKRILEIGGFRFRPYTIIACPTCGRTDTKLDIL
                     QLTQEVESMVKLHFEKTLIEKHKHITIAVMGCEVNGPGEARHADIGIAGARDGRVVLF
                     ARGEKKKILKASEAIKAIEEEIDAMV"
     gene            complement(38946..40508)
                     /gene="rseP"
                     /locus_tag="PLV81_02345"
     CDS             complement(38946..40508)
                     /gene="rseP"
                     /locus_tag="PLV81_02345"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOM08974.1"
                     /translation="MVTLTYILAAVILLGLCIFVHELGHLLGGKMVGIKAEVFSIGYG
                     KGFIKKKWGDTTYQITLIPFGGYCKFYGEDPNEERQGNPYEFLSAHPLKRVVTVLMGP
                     LFNLFFGIILFFIMNIHGYPVETNKIYIPEYLTKGEYVSPAYEAGLRTGDTIVAINNE
                     QIVGFTDIQSKVAFSKGKPITVKAIRGNDTLEVTIAPKKYTKKGYYTIGVMPYGDRVL
                     VMGILDNSPAKKAGLKEMDEIIEANGTQFHSPEEFTQFIRRHPDKELSLTISRGGHLI
                     TLPITPQTKEVLTINQFTDMRFPKESYTITVDSMSLIKAAANAGKVLINGKAVKSFEE
                     MQNIINASKGVITLKTPGGTYSGIMKFERYGFVGIETAISPENKFIQYGVRDAFVKSL
                     VDPFDFIALNFRGIGMMFSGELDVRQNLSGPIRIAKIAGDTAYYRGVSAFIILMAKIS
                     IILMVMNLLPIPMVDGSFILIFLYEAITRRAFSQKLLEKIQMIGFAILVLLGIFVIFN
                     DLSFLPFFKDLF"
     gene            complement(40517..41671)
                     /gene="dxr"
                     /locus_tag="PLV81_02350"
     CDS             complement(40517..41671)
                     /gene="dxr"
                     /locus_tag="PLV81_02350"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOM08975.1"
                     /translation="MHSSVIIFGSTGSIGLSTLKVIRQFGNRFTVKGLSCHKNITLLK
                     EQILEFHPQYVAVSDEETCSTDEYKKLKKEFGSIVFYEGLEGLSKLAANGADILVVGI
                     VGAAALKPTLDSLQYIKRIAIANKETLVMAGDIFLKLLQRYAVELLPVDSEHCAIFTL
                     LGGRNTDSVKRLILTASGGSVRHKPVEALDTVTVEEALTHPTWDMGKKITIDSATLMN
                     KGLEVIEAHYLFSVPYESIEVMIHPESIIHGMVELIDGTLLAHMSITDMVYPIQRALT
                     YPQVCHNDFGNVDLTAIGSLHFTKPDKKRYPALELCYEAGRQGGTMPAVLNAANEVAV
                     NAFLQKKISFTNIVSVVEKVLGKHRKIDDPDLQTIFNADAEARQLALTCI"
     gene            complement(41687..42574)
                     /locus_tag="PLV81_02355"
     CDS             complement(41687..42574)
                     /locus_tag="PLV81_02355"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOM08976.1"
                     /translation="MKERTKNTLIRILTAVVALPVYVFLIVTDSLYSLPVLLVSIIVT
                     GVCLWEFYQIAEGQTGYRPFVKTGMISALIINIIMYSYAYGNITGIGSLTVNFDVRYL
                     LAAVMIAISVFATIHIVKRPLKGGIYSLGATILGWFIIVIPYAHIMFLKSLRDGVLFI
                     IFVHLIVMINDTGAYFGGVYFGKHKANFKASPNKSWEGYFSGLLFGIVFAIIYSQLIE
                     VLYGRYFFSIVESAIIGIIFSIIGNIGDLFESAIKRDGAIKDSGSIIPGHGGMWDVFD
                     STIFAMPLFYYYLVIRGII"
     gene            complement(42571..43293)
                     /locus_tag="PLV81_02360"
     CDS             complement(42571..43293)
                     /locus_tag="PLV81_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009781585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOM08977.1"
                     /translation="MLIHYKRKIDYSNIPRHVAIIMDGNGRWAKRRGLPRIEGHRRGA
                     ETIERCMDTAIELGINVVSLYAFSTENWSRPKEEINGLWNLLESFFEKKLNTIIEKGV
                     QVRHSGSLKELPVNVQRIITDAVEKSKNNSRIILNFCLNYGGRHEIIHAVNEWLQKKP
                     SPELSLTPEIFEGYLYTRGLPDVDLLIRTSGEYRISNFLLWQIAYAELVFMKVLWPDF
                     NEKHLYQAIYEYQHRERRFGGL"
     gene            complement(43324..43881)
                     /gene="frr"
                     /locus_tag="PLV81_02365"
     CDS             complement(43324..43881)
                     /gene="frr"
                     /locus_tag="PLV81_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOM08978.1"
                     /translation="MIKDILDDVKNRMQKSVQNLAKDFATIRTGRANPAIFDNVKVEA
                     YGTEMPLNQVATISCPEPRLVVIQPWDRTNLSAIEKAILKSNLSLTPNNDGNIIRIQI
                     PELTEERRKEYVKMIKQKAEECKVAIRNIRRDGNDMVKDLEKSKEISEDDAKNALDEI
                     QKITDKFVAEVQTMTDGKEKEIMSL"
     gene            complement(43905..44627)
                     /gene="pyrH"
                     /locus_tag="PLV81_02370"
     CDS             complement(43905..44627)
                     /gene="pyrH"
                     /locus_tag="PLV81_02370"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOM08979.1"
                     /translation="MESESPAQYKRILLKLSGEALAGQQKVGINPEIVSHIAREVKRA
                     YELGVDVALVIGGGNIFRGSMGTSIGIERATGDYMGMLGTVINALAVQSVLENMKIPT
                     RVMTAIEMRQVAEPYIRRRALRHLEKRRIIIFAAGTGNPFFTTDTAAALRAIEIGADL
                     FLKATKVDGIYDKDPVKFPEAHKINAASYMDVLQQQLGVMDYTAVSLCMENKLPLVVF
                     NLFDEGSLVKILKGEDIGTRIS"
     gene            complement(44650..45249)
                     /gene="tsf"
                     /locus_tag="PLV81_02375"
     CDS             complement(44650..45249)
                     /gene="tsf"
                     /locus_tag="PLV81_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOM08980.1"
                     /translation="MAEITSEMIKELREKTQAGMLDCKKALIECGGDMEKAVDYLRKK
                     GLAAANKREGRVAKEGIIASYIHNNSKLGVLLELNCETDFVARNQDFQDLAKDLCMQV
                     AAANPLYVSPEDVPAEVLEREKEIYREQLKESGKPANVIEKIVEGKLTKFYSEVCLLE
                     QEFIKDNKVLIKDLIKNKIATYGENITVGRFVRFQIGQR"
     gene            complement(45297..46112)
                     /gene="rpsB"
                     /locus_tag="PLV81_02380"
     CDS             complement(45297..46112)
                     /gene="rpsB"
                     /locus_tag="PLV81_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005398068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOM08981.1"
                     /translation="MSVISMKALLESGVHFGHQTRRWNPRMAQFIFTARNGIHIIDLQ
                     KTMQRLKIAYAAMKEIAAKGGRVLFVGTKKQAQGAVKEYAQKCGQFYVSERWLGGLLT
                     NYKTVSRSIQRLKELEQMEATGKWEAETKKEILELTRELERKRKVLEGIKDMDRLPDA
                     LFIIDPKREAIAVQEARKLGIPIFAVVDTNCNPDEIDYPIPGNDDAIRAIALFLEVMA
                     NAIIEGQSAGQVSGEAVEAKIDFVEEEAAEAKPEKDYAEEYADEFDEYEDDRR"
     gene            complement(46332..46568)
                     /locus_tag="PLV81_02385"
     CDS             complement(46332..46568)
                     /locus_tag="PLV81_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08982.1"
                     /translation="MTFSRDHIGYFLGFVILGGILGIAIGSFIVKLAPSLAVINAPLT
                     RPIGFDLNLISFYIRFNISAIVGMIAGVLLFKWI"
     gene            complement(46640..46915)
                     /locus_tag="PLV81_02390"
     CDS             complement(46640..46915)
                     /locus_tag="PLV81_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HOM08983.1"
                     /translation="MKNLKKIPQFDNEDKEREFWSNNDATEYINFAKSKKMYLPNIKP
                     TTRSISIRLPESLIYKLKNIANKKDIPYQSLIKLFLAEKVNEEMKKQ"
     gene            complement(46884..47201)
                     /locus_tag="PLV81_02395"
     CDS             complement(46884..47201)
                     /locus_tag="PLV81_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HOM08984.1"
                     /translation="MRQIDFENITGFDWDEGNEFKNWVKHKVSVAECEEVFFNEPFFT
                     YVDEKHSLKEDRYFVLGQTDQKRHLFIVFTIRKTLIRVISARDMNKNERKIYEKLKKD
                     TTI"
     gene            47509..48321
                     /locus_tag="PLV81_02400"
     CDS             47509..48321
                     /locus_tag="PLV81_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013387555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOM08985.1"
                     /translation="MLKNLLTYNPVFLALVATLFTWAVTALGAAMVFFFSSINKKILN
                     SMLGFAAGVMIAASFWSLLNPAIEMAQSTGNTPWIPAVSGFLCGAAFLLVIDRILPHL
                     HMGLAIEKAEGVKTSWQRSVLLVLAITMHNIPEGLAVGISFGALTNSTDTGVLAGAIA
                     LAIGIGLQNFPEGAAVSIPLRREGFSRFKAFQCGQLSGIVEPIAGVIGAYLVSVITPL
                     LPYALSFAAGAMIFVVVEELIPESQTGNETDLSTIGAIIGFATMMLMDVALG"
     gene            48495..49877
                     /locus_tag="PLV81_02405"
     CDS             48495..49877
                     /locus_tag="PLV81_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011320326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HOM08986.1"
                     /translation="MTTVNEILTSQRKFFETGATKDIHFRLQQLATLRKSLKTYGNRI
                     IEALDKDFKKPKYETIATEIAVTIYEIDHAIKHLKQWAKPKRVKTPIVHLFSKSYIFP
                     EPYGVALIIGAWNYPINLVLNPLIGAIAAGNCAIIKPAYTAQESSKVLYDMINEFFPE
                     EYIAVVQKYPGVYDEILNQKFDYIFFTGSTDVGKTIMQAAAKQLTPVTLELGGKSPAI
                     VCDDADIDASARRIMWGKFINAGQTCIAPDYVLIHKNKKEEFIHASQKYITEFYGDDP
                     QASNDFARIIDNQQYDRLARMLEHCTIAVGGQTNKEERYIAPTLITNITLNHPSMQEE
                     IFGPILPVLEFTTIEEAAQIIKSFPKPLAAYIFTNNKRTWEKLIDDISFGGGCVNDTI
                     SHYVSPYLPFGGIGFSGIGQYHGKASFDTFTHYKGVLKKPFFIDTFLRYPPYNKGKVS
                     FIKRYFRVKL"
     gene            49954..50274
                     /locus_tag="PLV81_02410"
     CDS             49954..50274
                     /locus_tag="PLV81_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08987.1"
                     /translation="MGLALKTYEIFKENEAIAKGLAEIIDSLEQSIKQNESATKQDVR
                     ETELRLIKEIEQNRFQMKEIELSLLKEIETVRSSTIKWIIGLWFTQTIAIAAFIYTVI
                     VQIK"
     gene            50281..50817
                     /locus_tag="PLV81_02415"
     CDS             50281..50817
                     /locus_tag="PLV81_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08988.1"
                     /translation="MATQKKKNNKSKSQAKKTQPGSKSTATAATAQYIKAINLLLKKL
                     DVESLNFLYEQAKVLLHNLEVKKLQHEFDELDVKTITAKQKGYSKDKLEVVEADDLRH
                     FIFVINGARNFFSRDEMRKIVTLCHSAPTVKDGMRMLYHWFETNRKDVIIDTAIDGSH
                     DKALETIYNHIINNYTVN"
     gene            50874..51374
                     /locus_tag="PLV81_02420"
     CDS             50874..51374
                     /locus_tag="PLV81_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08989.1"
                     /translation="MNKDNIININTIDKTQRYIVVPRRIRGKVQKVVKPLKKVKGNCY
                     FTCNMPHALINFIYRSVKKLGLTSSNLIFSRGTVRINNRVVHNAVSSTVLDWDLGISF
                     IIPLKLRYNTFVTIEVDNNDYSVRLMELVILIALMAHAHPSKTRKKHIENAHKVLALG
                     WKSILT"
     gene            complement(51376..52905)
                     /locus_tag="PLV81_02425"
     CDS             complement(51376..52905)
                     /locus_tag="PLV81_02425"
                     /inference="COORDINATES: protein motif:HMM:NF039685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6206 family protein"
                     /protein_id="HOM08990.1"
                     /translation="MEDDHKDIIEHLELLNKVYELKRKRIRFMQLASVDTELKELSQN
                     FYKIWNKTSKGFVLKDTVQHSKSIDIIKGLFGQHDEIITMMRGNKAILTVDKIRFYQY
                     LVRHIANTRALHYLYNLFYGEENLSIEKLVKEQLPYLERYLPTFKKFDKTKNLEGLVL
                     SQFDVQNIWSIIEIYDRIRDNLIQDTSLYRQFTKDFTRLYREELKLKILGYGEISTVM
                     QTIHGSTLNQSFIKTKIAESDWVWKRMPPIDTLDAVEALKKVYHEYREILTKKIGIQV
                     PQQQFRYFKSNGWYTVYAGQKKINPQMVGNFLVKRLDEKNAVVLFNKILAELKKWYSF
                     NISDSSLKVGIDGQISNWVLISVDGALNYVGQNDTLVYIDTSTPLYRINGVEQLNAEL
                     FLKNTPWFLRAIIRALFLQEVLDRYYDLRSVVIDCIANLYKEKREDLIPAFIKAANFF
                     FASIDESIQPLTLDEIAKYYKSDAFIWRLFQFARRVDKFVTEVIFRKKYMYRLPEKIE
                     R"
     gene            complement(52916..54136)
                     /locus_tag="PLV81_02430"
     CDS             complement(52916..54136)
                     /locus_tag="PLV81_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOM08991.1"
                     /translation="MNKENIMELYARHVSSGKVEMYKKYDIALVPHKRRGVFLYDITG
                     KRYINCHCNGGVFNLGHRNKKIMDAVKKAMQRYDIGNHHLISQPKAMLAQKLAATMPH
                     GLNQVVYGVSGGEAIDTAIKLARAVTGRPNIISAIGGYHGHTGLALATGDEKFKKPFE
                     PLSPGFIQVPFNDVEAMRAALDEHTAAVILETIPATLGIVVPHKDYFKEVNKLCHSHG
                     ALLIMDEVQTGFGRTGKLWGFEHFDVVPDIVVLGKGMSGGIYPITATVYKEDYAFFFK
                     ENPFIHISTFGGSEIGCFAAMAVLDMCDSTLLNNVNEMADYFTKKLSMLSSQYPKASF
                     TVRGLGLMMGLAFNDEMTALFMVKLLFDNGVYVVYSGNDPKVIQFLPPLIITKREADL
                     VIAAVQKSLQALSQ"
     gene            complement(54232..55074)
                     /locus_tag="PLV81_02435"
     CDS             complement(54232..55074)
                     /locus_tag="PLV81_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08992.1"
                     /translation="MESFWQKIDTIDTITQGTILLRINNNLKKRQQGVFLQYDGGIIL
                     KNVIDHNNNSLVAAEGIIYPIQEDIFFKATSTFATSPLASKALKIIFEWPLYQKHSEL
                     SLHIEHFVKTTFVPEQILDYQKNDNLKSIFIPIQQYFRIGRYKERRNIERVCYEKFKF
                     WLDSLRVGEHITYLASVAGISSHTPKFYSAGTKPHEVTYQYLDHEEFAFNPTHGGHIK
                     LNAIIGGKKQFLVDAGSNYIGRGVKATLFTAKQVATALKKAYPEYEFIPLAGRGAFGV
                     EQSY"
     gene            complement(55140..57641)
                     /locus_tag="PLV81_02440"
     CDS             complement(55140..57641)
                     /locus_tag="PLV81_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOM08993.1"
                     /translation="MKNLLACCTAKLNICMNSIAYILTIVFIISCSSEDTNSLHLTSE
                     EIKYIQSLKKPVIAAPCPDYPPIDFTDHDGNHIGLSHDYLDEISKKLGVTFTVATIQS
                     WADVLQAAKDRKVDIVLSIQDVPERHSYLQFTKPYVRIPNVIVVQKSNTKVNNLDDLA
                     GKKIAAVKGYAVIDFIKKKYPSFTIEAVKNETEGLMKLSFGEYDAMIISLSTVSYLVD
                     TYGITNLRIAGNAGYDWNLCIGVRNDDPVLFSIISKALNSIPESKRRAIYQRWISLAP
                     RPFYKEKEFWYILTLLLFGGLLVIIGIIAWNASLRRQVKIKTQQLEHELYWHKKTQEQ
                     LLLEKERLLITIQSIGDAFISTDDKGNIILSNKQADVLFNNMISLGKDVRSLVNFTES
                     TTQKLVDPIGECIKLKKSVIYYHLKCQCNNRTIDVSVASSPIEIENTIIGAVTIIRDI
                     TDMLAMQNELIKLQQFESLGILAAGIAHDFNNILTAIMGNAEIASLMLLKNTKGNDVL
                     EIIDGIKKSVKSAQSLTGQLLTYAKGGKPIKKEGNIIQLIHDTVDFVTHGSSVKVGYI
                     VDGNIENFDFDEHQISHVFRNIVLNAIQAMNKSGVLTVTVKKVELKHDVLQLPDGEYV
                     MIKFSDTGKGIPQEHISKIFDPYFTTKEKGTGLGLTTSLSIIRQHGGTITVSSSDKGT
                     EFTVYLPKIKGTVSVNENHNARVDSISAHVLILDDRQEILDVTAGMLDILGCTYDTAS
                     HSDDAINKILTAKERGKPFDCVLVDITIPGSISGVDAFAQMKKIQEKLKGIVMSGYAD
                     NSAIADYAQYGFSWYLVKPFTFDDLKQALIKATIA"
     gene            58193..59335
                     /locus_tag="PLV81_02445"
     CDS             58193..59335
                     /locus_tag="PLV81_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08994.1"
                     /translation="MKKIFFMFLFFTYLLSGLGFTENNIKKREVQVKPNVQNVDIEFN
                     GEYLKGYLFIVDARTDEEKLKNFNERNANGSIFIFFQGHAQRADDAYKFTKELALKSK
                     SGITIVPLCDTPYGKNKDLRGDNGKEIILFYLIKHALKSLNMNVDEEIQCKDIKIDGK
                     SFVDLTNSIKVKLTAIGWSHGGLLARRMASKYTSFENLIQICPAGFTQWPDNSCQASC
                     CVLSSFNWESLRIGFTETFKGHFCDVMGAGWGITKGLTGDTARSCNSCIYGNFNILKI
                     FRPYKDIADVTLYADDNNFPVVNKKHILVIFGEDDSLFDDEKLLKYDKNSEIIKEQFF
                     SKYYEKSIISNTKLDVRILPGKHIGPITHYDIYVDSIVTFLDEKLI"
     gene            complement(59617..60903)
                     /locus_tag="PLV81_02450"
     CDS             complement(59617..60903)
                     /locus_tag="PLV81_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08995.1"
                     /translation="MNKYKCRFIIMLVLSVLSLMVQLHSRLTMVYAQVSSENGDISFS
                     IGGYYKNLFMGYKSNEYYSSNYIPEEKLLVTDVNRVRLSPHCDIGQNIVFHADIDNEF
                     IISNYTQSKTFDATWIGFSYDQLYTDYYDYRNDTGYYRLKLHRAFAKCIIGKATITLG
                     RQLVRFGSGKIWNPLDIVNPISPLSFEGAEETKGVDAMRFELYPAHFLEVGFVYVPQR
                     KNNEFGDSFDNSVYLLRNKVVLSNIDFAILAGKIMQRNVAGLDVALPVYDGLLRGSFL
                     VDKDGDNTYYIAGTGYDYSFESGFSILLEYFYNGYCLTKNDKLYQAYYETFIYGMNEQ
                     RYRTLAYHFLTYNRHYAGLSVSFSGASLFSASATSILDFEGHGVMLIPSVVYNIVEDC
                     DLTFYMLYAIVAGNNLSDFSSFDNRPALAAEVKYYF"
     gene            61227..61694
                     /locus_tag="PLV81_02455"
     CDS             61227..61694
                     /locus_tag="PLV81_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08996.1"
                     /translation="MSLKKQLSRSIIAFSTLIFISFGILFGSFVYYNYQTSISLLMKQ
                     QNIALKYFIEGYFIKMYNYIYISSNNDAIANVLLRDANNKRTVLKIFKHFENADRDIN
                     YLYSGYKDGTLLINDYTPPKGFDPRVRPWYTIALKTAPEISNGLAYQEIKTKQ"
     gene            61737..62312
                     /locus_tag="PLV81_02460"
     CDS             61737..62312
                     /locus_tag="PLV81_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM08997.1"
                     /translation="MCGVIAIDTSLEKLARIINTTNKGFSSQHSFVITNDGTIIIHKN
                     PKMLRKNFFTLSNITYTASGASYSYTLDDIKKTAYIQDINKIGWKVITEVDNSEITNI
                     VITRFFISIMVITLIASLFGLYLNKIFTHDIITPILSLAKRVANIVEAKSVDDKYSYP
                     DNEIGVIAHNLEQLTKEKLYQKNMELTQLNK"
     gene            62320..62805
                     /locus_tag="PLV81_02465"
     CDS             62320..62805
                     /locus_tag="PLV81_02465"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004100852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOM08998.1"
                     /translation="MDLSYIDELTSLNNRRKMEEELIKEFARYTRYGRMYSVILIDID
                     YFKKINDTHGHDAGDFVLKELAHIFKHNCRKTDTIGRWGGEEFLILSPETNISEAYNV
                     AENIRSIVEQHLFTMIDRVTISAGIGGSFPDMIDIYDIIKKADENLYRAKQTGRNKVL
                     Y"
     gene            complement(63123..63818)
                     /locus_tag="PLV81_02470"
     CDS             complement(63123..63818)
                     /locus_tag="PLV81_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOM08999.1"
                     /translation="MEIIQVVNCTKDYHSDGQIVHALRGVHLSLHKGEFAAIAGPSGS
                     GKTTLLNCIGGLDYPTQGNVYIEGTDISKLSPKELSRLRLFKIGFIFQAYNLIPVLTA
                     LENVEYILLLQGMGDSYREKKAAEVLELVGLKDEMHRLPHELSGGQQQRVAIARAIVS
                     SPAIVLADEPTANVDQKTGSALLDLMEHLNKEKQITFLFSTHDPMVMKRAHRLITITD
                     GTIVKDVKQKKSR"
     gene            complement(63833..65077)
                     /locus_tag="PLV81_02475"
     CDS             complement(63833..65077)
                     /locus_tag="PLV81_02475"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOM09000.1"
                     /translation="MIRMQNVKIVLMIGWRNVFRNKRRSLIVASSIVVGLVVMMFSMA
                     IMNGFNRQMLENTINTSLGHVAIHKKGFFNDMKLSLSFVPKDLCNAIKNIQGVACIGQ
                     RVKFEAMVQSSRSSQGVLVMGIQPEEEKCVSNIFEYAGSDIHSYITDSSNNDILISRS
                     LATKLDIGIGDKCVVMLQDKDGVLAAHAFIVKGLYQSPIESFDKYVVYTGIRKAQEIS
                     GLGNAISEIIVRCTDKDFADSVASTINSLDTADLEVLTWKQMAPNIVQAIKLFDTMMY
                     VFLAIIFISIIFTIANTMIMAIMERYREIGIMKSIGTQPSWIAAIVILEACGLGVLGI
                     VAGIVITYLLVAITATTGINFAFYSESMRVWGTGTTIYPFLTATNSIVATVIVLLNTI
                     VASLYPAYKAAKIKPIDALHFI"
     gene            complement(65093..>66261)
                     /locus_tag="PLV81_02480"
     CDS             complement(65093..>66261)
                     /locus_tag="PLV81_02480"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOM09001.1"
                     /translation="LTLCTIVVGCAMIILLNAFAKGGHDQMIEDATGLVMGHIHIHEK
                     GYWENKTIDYAFEADESIEKALSSNTHIRAYCKRIMCDALIAKGGTTKGVMIQGVEPV
                     REKQVCGLHKCIIKGRYLSDSDSTHAVIGATLAENLKVDVGNTIAIISQGFDGSIAAE
                     HLTINGIFKSGNPEYDKFLLQMPLKQADETFSMMGYIHAYVIKASHIDNIDAITSSLS
                     KKLQNDVEVLGWEKLMPELVQFIIMDDVSAYLFDLMLFLVVAFGILNTIQMSVYERTR
                     ELGIMKAVGTTPGQIVALVILETFFITVIGVLLGVALGAGISYYFTVHPIDYSQYSEE
                     IAVWGITTTIFPATVTIANIVVTVMLTMIVSLLFCIIPARRASRLHPVEAIRHI"
BASE COUNT        21004 a        13232 c        10775 g        21250 t
ORIGIN      
        1 tctgaagttg aagaaggcag agattttact gattacatta atcctgattc attaaaagtt
       61 gtaaatgggt atatagaacc ctatgtacaa ggtgcacagg tttacactaa tttccagttt
      121 gaaaggttgg gctattttac cgttgatcct gattctaaca atgacagact catttttaat
      181 agaactgtaa cactgaagga tacatgggca aaaatagctc aaaaagaaga ttgaatacag
      241 tcaaaattat tgacaaaatt tttgtttaca gaaactatgt gctatagatt gtttatatga
      301 aattgcccgg tggtgtaatt ggcaacacat ctgactctgg atcagagtag tccaggttcg
      361 agtcctggcc gggcagcatt tatgtaataa aaatgcagag gaatcctaca aatcctctgc
      421 atttttaatt taaggtttct ttatcagtaa ctatcgccat gtattctatt gcattaataa
      481 tatttataat aacgtattca ggcataattt ttactcgcct tccatgggtt aatgttgaca
      541 ggccatctgc tgcttatttt ggcgctattg ccatgatttt aacgggagtg ctttcttttg
      601 aagaggctat tcgtgctatt gattttaaca caatagccct tttgctgggc atgatgatag
      661 tcatagcaac attagaactg gatggttttt ttgcctttat tgcacaaaaa actatatctt
      721 tttccagaac cccgttacag cttttgtgga tcattacttt cactactggt attgcaagcg
      781 catttctggt taatgatgca gttgtcctga tgtttacccc catcataata tcaatatgca
      841 gatcagcgca acttgacccc aaaccatatc tgatagccga aatattggct tctaatgttg
      901 gcagtgcaat gaccatcaca ggtaatccac agaacatgct cattggcatt tattcaggaa
      961 ttccctatgt atattttctt cttcacctgc ttccagtttc aattattggg atgataatta
     1021 ttgtagtatg catcaaacgt atgtattcac acacatttga aacacagcag gtattgcagt
     1081 atagccagaa cgactacaaa tataacctgt catcgatgat attttctgtt ccaattttta
     1141 ttcttatcac attatttttt ctgcttcatc acgttcttga tatatcaata ccactgattg
     1201 cgctgactgg tagttcatta attttattat tgggtaaaat taaaccatca aaaataatca
     1261 aagagataga ctgggtttta ttattattct ttgcatcact atttatagta gtcaaaggtg
     1321 ctgaaaaagc tggaatcctg tcattattgc tgaagttttc ccactttaat aacagcctac
     1381 acagtataac ccttttacat gctgtcagtc ttgttttttc acagatagtc agcaatgtac
     1441 cttttgttat tctattaggg ccaaccttaa aatacagcaa cagcaacatc atatggttag
     1501 cgctggcctc atcatcgaca ctggctggca atgcaacaat aattggtgca atggccaatt
     1561 tgattgtgct ggaatcagcg gcaaaatcag gtattcaaat aagctttatg gaattcttaa
     1621 aacctgggat aattgttaca gtattaacat taatgctatc aatcgctgta ctatattttt
     1681 attcagtagc taactcactg tgaccaatat tttttaatct tttaaataat ttatttgttg
     1741 acatgtataa ttcaatacct taacctaaat aacaagaatt ttgcagttat aaaacatata
     1801 tatcaggttt tttactgttg ctaattcata atttatttga gttatcacat gcaaattgat
     1861 gtacacaaag aaggaccctt attaatattt acgttaaatg gtcgatacga catttctgaa
     1921 gtgcataact ttgaaacagt ttatcgtcat catattgaag ataatccaaa gattattgca
     1981 cttaaccttc gtgacctgaa atatatagat tcttcaggta ttgggtcgct ggtacgatgc
     2041 atgaatttag cacttaaaag caatattgaa ttcgtctgtt atgatataaa tgaaaatgtt
     2101 gaaagtattt ttaaaatagc taaactggac cagtttattt ctatcttaac acaggaagaa
     2161 tttataaaaa aatatatcaa aaagcaataa actcttttaa tggttctttt tcttccagca
     2221 cattaatgag tgcatcaacc acttcaggat caaactgtgt acctttattt ttaagtattt
     2281 cctgttttgc ttcataaagc gatagtgcct ttctatacgg cctatcactg agcattgcat
     2341 ccagtgcatc agcaactgca ataatctttg cttcaacagg aactttttca ccaggaagtt
     2401 gataatagcc tttgccatct gcacgttcgt gatgatacat tattattgat gcaatatctt
     2461 caaggtaatc aattgatgat aaaattttca ttccccgtat aggatggctt tccatcatga
     2521 gtttttcttt ttcatttaga cggccgtttt tctctattat ttcaatgggg atgccaattt
     2581 taccaatatc gtgggcagct gcagcatgat taagtaatcg cattttttcc tgtgacaaac
     2641 ccaaacgttt tccaattgcc aatgcatagt gtgcaacacg tgtggtatgt ccgctggtat
     2701 aaggatcttt atcctccatt gcactgatta acactgttat tatttcaaac actcgttcat
     2761 tcagcttctc atcaaatgat gccggcgtgc tgttttgtct catcattaat ttctgcatgt
     2821 agcgcaacac attatcaaaa cgaccattat caatagagaa tggataggta aagttacctt
     2881 tttcaaactg tttcagaaca gttaaaagcg aagcagcaac atcattgtac cgtttctgca
     2941 gcattttatt aacaatgaca aaagatatac tcactgccac tatgtttgac aaaatcaata
     3001 acataatttc tgaaaagaaa cttatggcaa tgtaattgag agcaaactga tgtgccagca
     3061 aaaacagcac attgatagta actatcgcac ataatataat taaatatatt atgactgtta
     3121 atttttttct tttaagatac ttataggtaa aaagatttgc cacacccatc gtaatgcctt
     3181 tacactgtat tcagtaatta taaaatactt aacatctttc caaatatcaa attattgtta
     3241 tttttttata agcgatagtt cctgttaaat taattattga cgataattgc atactcagta
     3301 catctgttta caatctgcat acacaacaac gtgaccatac tgaaaaaggg gttaaaaatg
     3361 tcaagaatac tttctggcat acaaccgtct ggaacgcttc atattggcaa ttattttgcc
     3421 atgatgaagc caatgattga actgcagcac aataacgacc ttttttgttt tattgtaaat
     3481 taccatgcat tgaccagtat tcacaatcca caggaactat ctgaaaacac cattaatgct
     3541 gcaatggact ttattgcttt aggtcttgat cctgataagg tgcatttctg ggtacaatcc
     3601 gatattcccg aagtgacaga gctagcatgg attttatcat gccacaccag cttaggactt
     3661 ttagagcgca gccacagtta taaggataaa attgcaaaag gcattgtgcc taacacaggc
     3721 ctttttacct atccagtgct tatggctgct gacatactgc ttttcaatgc tgaagttata
     3781 cctgtgggca aagaccagaa gcaacactta gaaataaccc gtgacattgc tgaacgcttt
     3841 aaccagatgt acggtgaaat atttgtgttg cccgaaccaa tgatatcaga ggatattgca
     3901 gtcattcctg gaatagatgg ccagaagatg tctaaaagct ataataacac cataaacatt
     3961 tttgaagacg aagccacact caagaaaaaa gtaatgcgta tagtaaccga ctcaacacca
     4021 gttgaggcac caaaaaatcc tgacacctgt aatctgtttg ccctgtataa attatttgct
     4081 gaaccagcaa aaactgaaat actcaaagaa cgctaccgta aaggtggcat tgggtatggt
     4141 gaggtaaaaa aagagttgtt tggaatgata tgggaatatt ttgctccctt cagagataaa
     4201 cgaaaacaat tacaaaacaa caaaagtgaa atagctgcaa ttttaaagaa gggtgctgaa
     4261 aaaacccgcc ccattgccca ggaaacacta ctgcgcgtta aaaaagcagt ggggctggat
     4321 tatttacaat aactattgct tcttaattac cacaaacttg tggatacctc cacgcttgac
     4381 cagaagcagt aatgtttctt tcttttcata ttgtttcagc aattcgttat atttatcaac
     4441 attttccaca ggagtatttt caatttcctt gatgatatca ccaaccatca tgcctgcctc
     4501 atacgcagga ctttgctcct gaatttcaag tacaattact cctgtagtat cagtaattct
     4561 gaatttatag gcattctctt cagtaatatc acccacacgc atgcctattg tatcaacttc
     4621 ttccatcttt tcttctggtg cctgtgcctg tgcaggaagc tcagcaatga gtacttcccg
     4681 ttccagttcc tgaccacccc tgaatatttt gactttggca gttgaacccg gttttaatga
     4741 agcaacatat cttcggagct gatttatatc agacacttca acgccattga ccgaaataat
     4801 gatgtcgcca atttcaattt ttgcattttt tgcaggtgaa tccttcatga tatcggcaac
     4861 caaaacaccc tttcttcctt caaacttgaa gttttttgct atttccggtg taatgttctg
     4921 aatatatacg ccaagccacc ctcgtgttac cttgccctta ttaatcaact catccaccac
     4981 ctgttttacc atgttggatg gtattgcaaa ccctattccc atatagccgc catttcttga
     5041 aaatatagca gtattaatgc caataagttt accattaatg tttatcaggg caccaccgga
     5101 attaccggga ttaatggcag catcggtttg gatgaaatac tcataatcgg caagtccaaa
     5161 tccttcccgg cctgtagcac tgataatacc catggttacg gtatgcccaa atccaaacgg
     5221 attacctatt gctattgcta tctccccaac ttttaatgtg tctgaatcag ccatgacggc
     5281 aactggcaaa tcctgaggaa ctgtaccgga aattttaatg actgcaatat cagttgcggg
     5341 gtcagagcca accaactcac aggggaatgt tcgtttgtca aataacgtaa tggttatgga
     5401 cttagtattt tttaccacgt gattgttggt aactatatat ccgcgtttat caataataac
     5461 acctgacccc agtgcctttg cttctttggg gaccgggaaa ggcataggca tccgtttgct
     5521 ttcccgtggg ctgccagcct ttttttctgc tgatatactg acaacggttg gaatagcact
     5581 attggttacc tttaccagtt gttcattaaa ttttgataaa tcggaaatat tctcagatcc
     5641 ctgagctttt tttacagttt ttgcatcagt taatggtgcg gtacatataa taaaggtaat
     5701 gactgtaatt aagactattc gtaatttcat acaattatcc tgtttatata aaaaaatatt
     5761 ctatcagaga aataaacttt tctgactgta aatacagtat atacctataa acttatcggt
     5821 atgtaattat gctatcgtat attgttttgt tacaatattt acgataattg tcaagaatta
     5881 tgaataataa agtttgcatt attgccatct ttatttacag gaatacatgt gtccattttt
     5941 tacaaaattt atattcagta attattaata accaacttac agtgttaaat taatataata
     6001 actaacctaa attggaagaa attacttaaa tgaaaatatt taatatttta caaaaattcc
     6061 agtaaagcga tattttgtca tatcccatga tttaaaatac aattacgtta ttatatccgg
     6121 atatacaata tgaaacataa atacacattg atacttacca ttattgttgt atcacttctt
     6181 ctattctcgt gttcacgtca acaacgttat tcactatcaa aaatggcaat gggaaccatt
     6241 gtgaccataa ctgctgatag cacctcaaac aaaaaagcgt atgaagctat taatcaggct
     6301 tttgaaacta ttaaacacat tgaagaaata atgagcccct acattccaac aagcgatgtt
     6361 acacatatca acaataacct gcaaacaaat gtcagcaatg acacattcac tgtaataaag
     6421 ctggcactgg aactatcgca caacactcat ggactatttg atattacctt tgcctcagtt
     6481 ggttcattat ggcagttgca atcaccatca tttgttccgc cttcacaggg aagtattaat
     6541 caacgcctga attgtgttgg ctatcgctat atccatctaa atcctaaaac aaggacagta
     6601 ataaaacaaa aaccgtgtgt taccattggg cttggcggaa ttgccaaggg atatgctatt
     6661 gcacaggcag ctcttgtatt aaaacagtgc ggcatacagc aagggattgt ggaagctggc
     6721 ggtgacctgc aggtactggg cgataattac gggtatgggt acactattgg cataaagcat
     6781 ccacgccagg aaggcatcat tgggaccgtg attttacacg atgggcaggc tattgccact
     6841 agcggtgact acgaacgata taccatttac aaaggaaagc gatatcatca tattcttgat
     6901 cctgcaacag gctaccctgc acagagcgat attatttcag tttcggtgat taccagtaac
     6961 cccacaatat gcgatggata tgccactgcc ttatttatta tgggaactca gcaggcaatt
     7021 gccttttgta aaaacaatgc tgcatttaac ctgtattgta ttattattga taagaatttg
     7081 aatatctatt gttcaaaaga tcttttacaa agagtatcgt ttcctcatca ttcagtatac
     7141 ccattttaag attttacttt atatagatat tatcgtttac ttatcaatat gtatgtataa
     7201 ccatatcggg aaaccatgaa cacattcatt tgatggtata agcatgcatt atgaaataat
     7261 tatgccataa ataattaatt ctaatgtact tttttataga tgaatgttat tgttgtgtat
     7321 ataatttttc taatagttgt tcaaatattt tataacaatc tgctaacttt tatttaaagt
     7381 aactcatgta taaaaatatt taattagcaa actacattga gcttaattct tttgttaaac
     7441 ttgtttttaa tgacgtgaat attatcttaa gttaagtata tggagatagt tatgccctat
     7501 aaaatcgctt tgtaccctgg agatggtatt ggtcccgagg ttgtagcaga agcagcaaaa
     7561 gttattgatg cctgcaacat tgatgttcat tacacaacat ttaactggaa tacaaccctg
     7621 tataataaaa caggaaaatg tgcacctgaa aattatcttg aattactttc atcgtttgat
     7681 gcaatattat taggcgcact tggcaataca cataatgccc ctgaccatat tgctgttgaa
     7741 ccgcttctaa ctatacgaaa agcttttgat caatatgtca atatacggcc agcaatgcta
     7801 tttcctggtg ttgaatcacc tttaaaaaac aaaaaaccat atgacataga catgatagta
     7861 atcagagaaa acactgaagg tgaatataca gcactgggag gaagacatta tattggtact
     7921 cccgacgaag ttgcaatgca aattaactat ttttcgcgaa aaggaactga acgcataatc
     7981 cggtatgcat ttgaaactgc ccgaaaccgc aaacgtaaaa ctctgacaag cataaccaaa
     8041 tccaatgcac tcaaatacag catggtattc tgggatactg ttttttacga agttgccaaa
     8101 gagtatcctg atgtaatagc atcatcaatg cttgttgatg ctgcagccat gaatatggta
     8161 cgctgccccg agcaatttga tgttgtagtc gcatccaatc tttttggcga tatacttacc
     8221 gacctggcag cgatagttgt tgggggaatg ggatttgctg caagtggcaa tatcaatcca
     8281 acacgaaaat acccatcaat gtttgaaccc gtccatggtt cggcaccgga tattgcaggt
     8341 aaaggtgtag cgaatccagt tgctgcaata ctcagtggtg cactcatgtt agaattttta
     8401 ggtgagcacg atgcagcaca aaagattaaa tctgctgtgg tcaatcactt aaatgatggc
     8461 actgttaaga cacccgatcg tggtggtaat gcaacaactg ctgacgttgg cacagatatt
     8521 gcacagcgta tacaatgaaa tgcttgattt ttgtttttgt atatgatatt ctcttataga
     8581 ttgcaataat acttaattag agggttacca tggctttgac ggaatacaca ataccatcaa
     8641 caaaaatcaa tgattttttc atatctgttt ctgatggaat ggaattatat tgtatagatt
     8701 ttattcccaa aaaggattct tcatccaaac caattatatt ctttgttgca ggctggatat
     8761 ctcatataac cggttggaaa gatgtcctgc taactttaac cgatgaatac cgtgttatat
     8821 atatcgaaac ccgcgaaaaa acatcttcca gaatacctga agggaaaacg tataaagact
     8881 tttcgtttaa tgtcatgcga atggtacttg atctccatga agttattcaa aaagttattc
     8941 ctaaaaacag aacatttgtt atggcaggaa gttcgctggg ttcctcagtt atactagaat
     9001 atttagcgat gcacacccga aagccagaat gcgcactgct cataggccca cttcctgaat
     9061 tcagatttcc ccctctgctg ggtgatattg tacccaggtt tcctgcttca tggtataatg
     9121 tactcaaaga acttgtaaaa tggtatttac gcaattttcg cctggataca gaaaaagaaa
     9181 aggaacaaat ggcaaaatat accaatacaa taaattcagc agatccttat aaattaaaac
     9241 ctaatgcgct tgcactcaaa aattataatc tatgggacaa actccccttt ataacaacac
     9301 cctgctacat ctttggagca aaaactgata aactacatgc aaccgatgac attaaacgtt
     9361 tattgcaaaa tctccagtat gctgaatata ttgaatgtga aagcaataaa gaaacacata
     9421 gcagggttgt tgcaattcat tttaagaaca tcatttcaaa aaagctttat actaaaataa
     9481 aacccaaaaa gtagcaactt tgcttgattt tttcatatat tggttttata ttgagtgtaa
     9541 taaatattaa aaggaaatag tatgtccttt tgtaaagttg agatagcaac tattgtcctg
     9601 gatgaggaaa gcaacactcc tatagtggta ctccaggata atgaaacggg caatatactg
     9661 cctatcatga tagctccact tgaagcaagc cttatagcta ttgaactgga aggcaaaaaa
     9721 cctgtcaggc cccttacgca tgatcttatt attaccatgc tcaaaacttt ccagtacaca
     9781 ttgacatcag tcataattga tgaccttaag gataatattt attttgcaaa actcaatttg
     9841 attgctcctg acaactctga gattatcata gattgtcgcc caagtgatgc tatggcgata
     9901 gctctcagag cacatgtccc aatatatgtt aaaaagaaag tgtttgcgat agcgcaagga
     9961 ttagaaaatc tttcccagat agataaagaa accatgaaag aggtccttga agatattgac
    10021 attgacgatg tcggtggaaa aataatgtaa cattgttata ttgtggaggg cttatggacg
    10081 ctgagaaaaa tattgctgca cttcataata ttatacgaga ccttaccgaa atagaatccc
    10141 gtttaaatga atacattgac acaaccatgt gggtaagcga tccacatggc gctggtgaca
    10201 gatttgtatc aatcctcaaa gggcgttttg gccttgtatg gagaatatgc tacgaggctt
    10261 tgccaaaaac attctcaaaa gaaaaaattg attatctggg aaggataatt cgtaaagaac
    10321 ggtattttga agatgaggta taccgtcttg acaggcagga tatcatatca tcactcatca
    10381 gaattgttca gtaccgtgtt cagaatgtac gggattttga tgaaataagg aataacatca
    10441 ataaagattt aaaacatgta cttgaaaacc ttatactaaa ctatcctgtg ccaaatatga
    10501 tttatgaaaa tgaattaatt gcagataaga ttataagttc actgtgtaaa atagttaagc
    10561 aggttattct gggacatctc attgtacttg gcgatgtatt tgaccgtggt gatgaaccag
    10621 ataaaataat tcgtattcta aatcaaaagg atataaaacg gtatttaaca tttatatggg
    10681 gtaatcatga tatattgtgg atgggtgccg cagcaggtaa taaatcactt attgctgagg
    10741 cattgcgtat aagcactcgc tatgataacc ttgcattctt agaccgaata ggaataaata
    10801 tcacaaaatt gaaagagttt gctttacata cttatacagg agaaataccg ggtaatttca
    10861 aagcaaagca ggatatatca cgaagaatgg agaaagcact tgccattatc cagtttaagc
    10921 ttgaagaaca aactatacgg gaaaatccag aattcaatat ggaatcaagg ctctggcttc
    10981 acaaattagc agaaatgtta aaaaataatg atacttctgg attaactgat acacagtttc
    11041 ctacaataga tttagataat cctgataaac tatcacccga agaagaagaa ataattaatg
    11101 accttacata tcaatttaca acaagcaaga aagtccgcta tttaatggaa tttttatttg
    11161 aacatggcaa actataccat attcacaatt atatacttaa catacatgcg ttgataccca
    11221 gcacacatga tggtcagttt gaagaatttt taggttacag aggaaaagca ttacttgatc
    11281 atttgcaaca tagaattaaa acaataggca aaaattatct ggaaggcaaa cctcaaaatc
    11341 ccaaagacct tgctcttatg ttttatttgt ggtgtggttc aaaatccccc ttctttggga
    11401 agaatgccat gaaaaccttt gaacgatatt tcattgctga taaatccact cataaagaaa
    11461 agttgcttta ctggggtgaa aacctgctta aaaaggaatt catggataaa atcatggaag
    11521 aatttggagc ggaaagaata gtgtatgggc atacaccagt agatgtgcga aagggcgaaa
    11581 aaattgcatc tcctgacggc agggccatta atattgacgg cggattttct gatgcgtacc
    11641 tcagcagagg gcatgctctc attcagactc cgtattcact ttatgcaata attttaccca
    11701 cccctgaaga aacaatacag gcgcgccaga aaaaagaacc tgttaattta atggttgaaa
    11761 tcattgataa ttttgaaaca cctttaaggg taaaagatac atataccggc aagcttctaa
    11821 tcaaaaaaag gaatgaatta tacgaaaaat tacttcaata ctccgagcac tattcagaca
    11881 tggagcataa tacaactcta aattattcaa ttgtataatt atttgaacta taaaaaaacg
    11941 ggcagtttag tctgcccgtt tttttattat caccttttag caatctttaa acgtgccttc
    12001 aatttcttta attcaatgtc tatatttaac ctgtcagcaa atgtttcagg ttttggcaat
    12061 gcagctatct ctttaatctt tgattcaagt acttctggct tcaaatcctg tttgctcaca
    12121 gcttcctgag ccagaataat gagtgaatta ttcttaattt ctacaaaacc accatctaca
    12181 taaaaatatt ctgtactatc gccggatcgc aatcgtattt caccagtacc caactcagag
    12241 attaaagggg catggttata taaaaatccc atttcaccat catatgcctg taccaccgca
    12301 aaatcaacct gcccttcata tatctgtcgt tctggcgtta atacattgca atttaatttc
    12361 attgccatag ttctatcctc tacaatttat taattaccag caagcttctt ggctttttca
    12421 actgcttctt ctatggtgcc aaccatgtag aaagcctgct caggaagatg gtcatattca
    12481 ccatttatta atcctttgaa gcttcggata gtatcttcta atttaacata tttgccaggc
    12541 ataccagtaa actgctcagc aacgaagaat ggctgtgata aaaatctttc aattttacgt
    12601 gctcgctgaa caagcaattt atcttcttca gataattcat ccatacctaa tatggcgata
    12661 atatcctgta aatctttata tcgctgtaat attcgtttca cttccatagc aactgcataa
    12721 tgctcttcac caactatctc aggagatagt attcgtgaag aagattcaag aggatcaaca
    12781 gcaggataaa ttcctttttc agcaatctgt cgtgaaagaa cagtacttgc atccagatgg
    12841 ataaatgctg ttgcaggtgc agggtcagtt aagtcgtctg caggaacata aatagcctga
    12901 acagatgtaa tagaaccacg tttagtagac gtaattcgct cctgtaactc acccatttcg
    12961 gttgccagag ttggctgata acctacagcg gaaggcatac gccctaacag tgcagatact
    13021 tcagaaccag cctgtgaaaa cctgaatata ttgtctatga acagtagaac gtctctacca
    13081 gaaaggtcac ggaaatactc acacattgtc aaagcactca gtgcaacacg gagtcgtgct
    13141 ccaggtggct cattcatctg accatatacc aaacatgctt tattgataac acctgattcc
    13201 ttcatttcaa gccacaggtc attaccttca cgggttcttt cacctacacc ggcaaatacc
    13261 gaataaccac catgctgcat agcaacgttg ttgataagtt ccataataac aactgttttc
    13321 cctacaccag caccaccaaa taatcccgtt ttcccccctt taatatatgg ctcaatgagg
    13381 tcaataactt tgatacctgt ttcaaaaatc tctgcttttg gctctaactg atcaaatttt
    13441 ggtggctcct gatgaataga tcttctttct ttaacagtta ttggtgcacc tttatcaaca
    13501 acatcaccta aaaggttaaa tactcggccc agggtttctt caccaactgg tactgtaatt
    13561 ggagaaccgg tatcaataat tggagtgcct cttttaacac catcggtaga tcccagagct
    13621 actgcacgta ccctgttacc acctaaatgc tgtgcaacct cacatacaag ggttttcttt
    13681 tcacccataa cttcaacttc taactttaat gcattataga ttgcagggag tttatcttcc
    13741 tggaactcag catccagtgt agatcctata acctgtacaa ctttacctat attctcagcc
    13801 atatctaact ccttaattca tgaataatta gtattatatc atttaccctt tattcaaggg
    13861 ctgcagcacc acctacaatt tcagagattt cttttgtaat cttggcctgg cgagctctgt
    13921 tatatgttat agtcaaatct cttaccattt cttttgctgc atctgttgca ttcttcattg
    13981 ctactctacg tgcaaagttt tcagaaaaac ctgactccag cactgccaga tacagtttca
    14041 ttttaatata taatggtagc aatgaagaaa atattttata tggattaggc tcaaatatat
    14101 attctacatg aaaatcttca acactttcct gaatggccac actctcaggc ttcagtggaa
    14161 gcagccgggt aattgtagct ttctgtgttg aacgagtcat gattttggta tatgaaacat
    14221 acacctcatc aacttcaccg gtaacaaaca aattgatcaa ttcctcacct aattttgatg
    14281 catcttcaaa cttgaattta tcttcagggt ttaatacact tttatatatg ggctctttaa
    14341 tgaacttaaa ataatttgag ccctttttgc ccattgaata aataagtact tcttttcctt
    14401 cttcaatggt taatttattt ttgaaattta ttgtattatc aattacacgt gtattaaatc
    14461 caccacaaag ccctctgtta ccggtaatca taagaactaa tattctattt ggatccggtc
    14521 tttcctgcat aagtgggtcg tatacatctt caacgccggt ctgtcgcaga tgatcaatta
    14581 tggtttccag cttttcacca tatggctttg acatagttat cctgtcctgc atttttttca
    14641 tcttggcagt ggaaaccatt tccattgtct tggtgatctt ctgtgtattt ttgacagact
    14701 ttattcgctt taatatctct cgttgtgttg ccacagcttt tcctcaaaat ttagtatttt
    14761 taagccctaa catctgcctt tttaccggct aagtaacttt ctacaaaatc acccactact
    14821 ttgccaaatc tatccggatc ctttaattcg ccagttgatg aaataccgtc tagtaattca
    14881 ggatgagcct cacgcatgaa ttttaaaaat tctttttcaa actttcttac ttcagatacc
    14941 gggattttat ccagataccc ctgggtacct gcatatatga ttgccacctg ttcagcaact
    15001 ggcattggct catactgatc ctgtttcagt acctcaacaa gcctctgccc tctatcaagc
    15061 tgacgctgag tagccgggtc aagttcagtt cccaactgtg caaaagcttc aagttccctg
    15121 aactgtgcca aatcaagtcg taatgaacct gcatactttt tcatggcttt aatctgagca
    15181 ttgccaccaa ctcgcgatac tgaaatacca acatcaaccg caggtctgat accagaagca
    15241 aataatccag gaacaagata tatctgacca tcagtaatgg atataacatt cgtaggaatg
    15301 taagcagaaa cttcaccttc ctgagtctca attacaggta atgcggttag cgaaccacca
    15361 ccaaggtcat cagaaagctt tgcggcacgc tcaaggagtc gtgaatggca gtaaaagatg
    15421 tcgccaggat atgcttcacg tcctggaggt cttcggagta acaatgaaag ctgacggtat
    15481 gcattagcct gttttgaaag gtcatcatat atacacaatg aatggccatt tttttcgtac
    15541 atgaaatatt cacccattgc acaaccagca tatggagcta tatactgtag aggtgctggg
    15601 tctgaagcat tagcagcaac aactattgta taatccattg caccgtattg ctctaacttt
    15661 tcaacaactg cagcaacggt agatgctttc tgaccaatag caacatatat acaatacaca
    15721 cctttgcctt tctggttaat tatagcatct attgcaatag cagttttacc agttttacgg
    15781 tcaccaatta taagttctcg ctggcctcgt cctattggcg tcatagagtc aattgcttta
    15841 atacctgtct gtaaaggttc ttttacaggc tgacggtcag caataccagg agcaataaac
    15901 tctacaggcc tcatcttgct agaattaatg ggacctttat tatctattgg aatccccaac
    15961 ggattaacaa ctcttcctaa tagctcttcg ccaacaggta cagccagaac tctgtttaaa
    16021 cgcttaacag aatcaccttc ctgaattgaa aggtagtcac ctaaaataac agcacctatt
    16081 gaattttctt caaggttaaa taccaggcca tatgctccat tctcaaactc gagcatttca
    16141 ccagccatcg cgttatccaa cccataaata cgtgctatac cgtcaccaac ttgaataaca
    16201 gtaccaactt cccgtacatc taactgtgac ttataccctt caatttcctt tttaataatc
    16261 gattttattt cttcagtttt aatcttcata agccacttca cctctgatct tactttgtaa
    16321 taatttttca cgtaacttaa ataaatcgtt cttcaatgaa gcatctataa ttaaatcatc
    16381 tacttgcact ataatgccac ctaaaatagt tggatctata acttcttcaa caataatatt
    16441 cttattaaaa cgatcttcta agctcttttt gattctattt ataatatctg aatcaagctt
    16501 aacactgctg actaatttaa ttttctgacg attattaact atatcgagta attcgctgaa
    16561 agcgacacta atatccggaa tgtcctgttg acgtccatta tcaattaata ctttaagaaa
    16621 gttaaccata agttcagata gttcactgcc aaaaaccttg tcaacaaact gcttttttga
    16681 ttcctttgta atgcctggcg cactgaaata acgcataaga tcataatcat tttttaatag
    16741 ctcagcaagg aatgataact cttcctctac ttttgatata gcgttttttt cgtgcgcaat
    16801 ctctaacaaa gcacctgcat aaactttcgc tacttcactt gttgccacaa cgataaccct
    16861 actcattaaa atttaaggac agttcagtat cactgaactg tccttatttt ttgtaatgcc
    16921 tcttctacaa gggctttctg atcttcaact ttgagattct ttgcgataat ttttgcagca
    16981 atatcagtag atattgttac aacttcagcc tgaatctctg ccagcgcttt atcctttgca
    17041 agattaatct cgcgacgcgc tctttcaatg agatccttgg cttcctgatt tgccttttca
    17101 actatggaat tttttaactt ttccgcatca gccttacctt cagcgatgat gcttgccgcc
    17161 tcctcttttg ccttatccat cattgcttta tgctcagcaa atagcctttc ggcttcaaga
    17221 cgggactttt cagcagattc aatgtcacct cgaatctttt cagcccttgc atcaagcgcc
    17281 tcaacaattg gcttccatgc ggctttccat aatatcaata acaacactat aaaggttatt
    17341 attgtccata acaggaggcc gggatcaacc tttagcaagc cttcttttaa aacttccata
    17401 gtgaactacc tgttaataga tttacttaac ttcagtctgc tctgctgctg gcttctcaac
    17461 tggcgtagca attggagctt ttgttgcaag catgaatgtt acaacgagag caaagaaagt
    17521 gaaaccttcg ataagagcag cagcaatgat cattgctgtt cttaagtcac cactcaactc
    17581 tggctgacgt gccatacctt ctaaagcacc agtagctaat ttaccaatac cgagtgctgc
    17641 accaaaaact atcaaaccag ctccgaaacc cgctgcgagg taagaaaggc ctaaatattc
    17701 cattgactcc tcctcgtaaa tttttgagta ttaaaattta acaaacaata tgttattgcg
    17761 aacataatat attgaatacc tcaaagcctg atcattttaa tgagcatgca ttgaggcacc
    17821 aataaacata gctgaaagaa cagcaaagat atacgcctgg ataaaagcaa ccagtaactc
    17881 aagcaaataa atcattaacg aaccagccac agaacctata ccaacccaat agctctggaa
    17941 catcataacc agataaataa atataattat aacaacatgt ccagctgtca tatttgcaaa
    18001 aagtcggact gttaaagcaa aaggctttat caaaaggccg atgatttcaa ttgggaacat
    18061 cagaggcaaa acaaaagcag gtactccatg aggaaccaga cctgcccaat aaccaaatat
    18121 tccctgcttt atcatcccta ttcccagcat gcctaccagt gtacagagag ccaatccacc
    18181 agttactgcc agattacctg ttgctgttga catacctggt attaatccaa gcaggttaca
    18241 gaaaagaata aagaaaaaaa cagtcaggaa atatggcatt gcttttttta cataatgatg
    18301 gtcaaagtta ggctcaacca cttcatcatg aacaaaagca attaatactt cccataaatt
    18361 tacccatcgg gaacgagagc catatgatga atttttgatt ttacgggcaa cgggtataaa
    18421 aacaagcaaa cataaaaatg cagataacca tagcatcaaa acccatttgg taattgtcat
    18481 atcaaagata ccaaatagct tttcatgaaa aaatttttcg tttattttgc caatgaatcc
    18541 atcgtggatg gggtggtcag taaggtgatg catgactata tcatacatcg actcttcttc
    18601 gccactttta gatgattgta taatagcctg taaaccttgt agcagttgaa ccatagcctt
    18661 aaacacctca tcaaattttt ttattatctg attgatgcaa cgtaaacgtt acatccattt
    18721 acgcctttta gaaccgctac tattgttatt atcacccagc tgtgatttct ttaacagacg
    18781 atccagctcg gataaacttt tcatcaaatt ataaaaacct gctcccattc caataatagt
    18841 tccaataaca acaaaaatag gactgctatt gaaatgttta tcaagtaaga acccaccata
    18901 aagaaaaaca agaatcgtaa ctgccagctg tatgcctatg gttgaatatg caagtatgat
    18961 tcttgtgata tcccttttca cttacacctc aaatacaaac actgaattcc atacaagcac
    19021 catacctttt tttgtcaaca aaataagtct gatttttttt aaaaaaatta ttattgttat
    19081 catatacaat tttttgtatt tattatgatt tcttttgaat ttattaaaat tatctacaat
    19141 cctgcataaa acaaataaaa aaatttatgt atacatattg attgataact atttcatatc
    19201 gttaaagaat aatcagtcaa ctaaagcaga aaacccttat tttagatatt attttttttc
    19261 tttccagagt cgataaatta aaatgtgtaa cagcaacatt atcatagagg aactgtaatg
    19321 aaattatccg taaagctaat cgagaaaaac aatgaattta ttgccacttg ccctgaacta
    19381 gatatcagtt gttacgcttc caacaagatt gaagcaataa aaagaattca gagcatacta
    19441 tatttttata tgcaatcggc tcaggaatta ggattttctg ttgaaaattt tgaaactata
    19501 gatgttgatg gaaagcacta tctctttaac tatgatatct acccaccatt aaactccaca
    19561 atgcattaag catgaggctg gtctgctgcc caacctcatg cattcagcct ttttacttta
    19621 tatcattttc cttcaagctc atcttcatgt atagtaaact tattaatatg caacatatct
    19681 cttttccatt ttgctaaatt ttcagatctt ttttttctgt aaagcatact ttctatctga
    19741 tctttaacct tttcaaaggg taaaatttgt tttacctgtt ttcccttttg gttcacaaat
    19801 gtagtgaaac gcgtgtcttt atatttttca tattcatcac gaatttcctt gggggtaaca
    19861 gcaattcctc gctctccaat ataatccata tattctttgg caaaaagcat attttttctt
    19921 ttctggaatt ccttttgaag ttcctcattt gtttgcagat tctctttgat tgcttccctt
    19981 tttaataaag caaccttaaa ggcatttttt gcaatatcga ttttttttgt gtcatctata
    20041 gttgcctttg ataggctact tccttttggc tgaaaatatg aaatgcgctt ttccaggtct
    20101 gcaacagtaa acgaactatc accaacctta aaaattacat cttttggatt tttgctttta
    20161 caattctttt cattaaactg cacatcagat gcactcataa gtttttctat ataattattc
    20221 gatgctcttc gcaagaggat ctgttcaagc ctttttgcct gcatttcatc tttaattatt
    20281 ttattaagat tttttttggt tatttcctgt ttatccatta attgtataat gtaaatcccc
    20341 tgctgggttt ttattggatt ttcaataatt cctgatttac tgctttcgat agctgaagca
    20401 acttcaggtg caagcatatc ttttacaaca taaccaatat caccaccatt cttttttgaa
    20461 aaatcatctg aatacgtttt tgcaagctct tcaaatttct ggccgctttt tattttatca
    20521 attatttctt tggctttgtt ctcagcttca ctgtatgctt tttcaagctc agaagttgat
    20581 aaatttactc gtttattatt aacaatttta aaatccctta cacgtataag gatttgcttt
    20641 acatggtatg caggctcatt aaattctact acatccttaa ttttcttatt atataaatgt
    20701 ttaagcaaaa tagattcata agccatatct tccaaaaact tatacttttc tgtttcaatt
    20761 agattttctt tttttgcctc ttgtagggca atatgctcaa tagccatcat ttccagttta
    20821 tctttttgct gttttttgct tttgagtatt gcttctactg aaaattgctt atcttccaac
    20881 cattctttaa actggcctct tgttatttct accccatcat acgttgccag cacatcagaa
    20941 ctgcatttgc atcctataaa tccaataatt ataccaataa tacttgccac caataataat
    21001 ctcttcatat caattctcct gattttaaaa tatacaataa tgaaaataaa tgaaaatatt
    21061 ttatactaca acataaatat ggttatatag attattttta taattttagt acaccatatc
    21121 tatcattata tttatttttg caataaagtt ttttaataaa attaatatcc tgtaaggggt
    21181 attgcacaat gtgatatttc attaacccat ttataatgaa caatatggat aattatttaa
    21241 catccactcc aatataatct gaaacaagtt cttatatatt ttatctgata aatgaaaaaa
    21301 tttatttcaa atactattga cagaattggc catactatca taggatttac ttcaacatga
    21361 tattataaaa atcttgatta atgcctatgc gagacaaagc tttctacaga aataaattgt
    21421 atacgctctt acatcaggat cattcctttg attgtgaaac attaattgaa tatggctacg
    21481 atgcaattga aaagggtaat ttcagaaaag cattcagact ttttgcctta ggatatcgtt
    21541 taattggcaa ccaccccgaa atactcaatg ggttaggcct gacactttgt gaaatgggaa
    21601 aattaaaatt ttctaaaaat tttttgctat ttgcgttaaa aaaatttcca gaaaatgttt
    21661 ttattttatc aaatttagca accgtttatt ttgaagaata tgattatgat aaagcaatat
    21721 acttttatac acgtgcaatc aatgtcgacc cattctttat tgaagcacat ataaacctta
    21781 tcaatgtgta ttttgagcgt ggtgatattt ttatggctta catttcatgt cttgaccttc
    21841 ttaagaagga tccggataat tatgaagttc aggaattgtg taacgatatt atactgaata
    21901 tgggcttatc attagtgtaa tgttagcgtg atggcaattc atcactgata tctattttcc
    21961 atttcccgtc ctcttttacc aatgtatgga caacctcaag accaattgca ttttgttttt
    22021 tatgttttat acactgcaat gttacggttg cattattgtc tgttttttca actttaacaa
    22081 cttcccacgt tgcatctgta tcatggtgaa cccagataaa cgggtataca cttccataag
    22141 tttttttcat gttctcaatt ttcctttgtg atgcattgtt caaataggtc aataattgtt
    22201 tttcatcagt agttgtgaga tagcgtatgg ctatcattgc tgcatcatca accggttcct
    22261 ttgtacatga ataaaaccag gaaacaatta ctatcaatag tattacagat agaaggtttg
    22321 tatatgtagt tattttgtgc atgtgtgtat ataattacct ttaattaatt tataccattg
    22381 tatttaaacg caaagacgtg cgatagatat gattattatt tcacagtaga tacaatgcgt
    22441 tgagctatcg cccatgtatt attcccatga tttgcgatac gacaaccgtt ctttcaacgt
    22501 atagaaatca ctccctttgg gaacgtcacg aagtcgcgtt ttcagagtgt ctggatctat
    22561 aagctcgctg aacggaacag ccttaagatc aaaattatca gtaacggaaa ccatgacgcc
    22621 aaacatattt tcactgtaaa atttatacgc accatagcct aacatagctc ccagcaccac
    22681 atcaaagctt attggtggag cagagcgtgt ttcataacca atttgtttgg caacaatctt
    22741 tacttccatg cctgtttttt cctggtaacg ctttttcaca tgctcagcca gtatctcccc
    22801 aatttttgct tcacggtata atatattacc atgtttgtca gtgtgttttg gcttgtattc
    22861 atcgggcaac ttatctacaa ttccttctga aactgcaatg accccgtaag ggcggcgatt
    22921 gttttcacgt gccagtataa cgttggttat cttttctaca agcacctcga tatcaagcac
    22981 atcctcatca atatcctcag ctgcaaccat cataattgcc tccgctgcta tacccgcagc
    23041 ataggtaatc cagcctgctt tgcgccccat aagttcaaca ataaaaaagc tatcggttgc
    23101 ctgagcatca gctttgaggt ttaacatcat cttctgtgca gtatccactg cagtccagta
    23161 tccaaatgtc catggtattc cataatagtc attatcaatg gttttaggta tatggataac
    23221 aggataaccc ataagattaa ggaagtttgc tgtctttaac gtatcatcac caccaatgga
    23281 tattagcgca cctattttca ggtattcaaa tgcatccagt atatttttta gttttttatt
    23341 tttctcagca ttttgcaaat cgtcaagcgc ctttatatct tttccaggat ttgcacggga
    23401 agttttaagg tatatacctc tttcgttgcg gacatgagta atattgtaat ccagctcctc
    23461 gtagtggaca cctttcttta tttttggata attaatgtta aaatcctgca aaaattcata
    23521 gcccctgtaa aatccaatta cgggtatacc gttatccaaa aaattcaatg caactgatga
    23581 tataactgta ttggcgcttg gggccggacc tccggaaaaa agcataccta tctttttata
    23641 cgcaggacgc aatttcataa tacaatcctc ttacaataaa ttcaatacca aacaatccca
    23701 gttaaataat acttgtagat atttgtaata gcaattattt ttttttatga tttcaacaca
    23761 atcaatgcat caacagcaac tggtttattg ccatcaataa taacaggatt tatatcaact
    23821 tcggcaattt cttcatattt gctgacaagt tcacttaacc ctaccagcgc ctttgccagt
    23881 gattccctgt caacagctgg agaacccctg aaaggtccta ataatttttt tgtgcggagt
    23941 tcatccatca tttcataggc atcaagttcg gtaattggtg caactctgaa tgatacatcc
    24001 tgcagggcct cagtgaacac tccacccaat ccaaacataa cacagggacc aaactgtgga
    24061 tcgcgaatgg ttcctaacac aaattcacgg ttgtcaccat caagccattc ctgtaccaga
    24121 actccgtcta acggaacacc ttttccagtt atttcatcat atgcccgtgc cacctcttct
    24181 tcagttttta attttaattt taccattccc agttcggttt tatgtgttaa tgtatcggaa
    24241 caccctttta acacaacagg aaatcctaac tcctgtgcaa atgcaatggc ttcttcacga
    24301 ctttttgcaa gatagtttct tgaaacagga atacctgcta tcttaataac actttttgag
    24361 tcaaactcgg ataatgcttt ttgtttatta gcaattgcac gctctaaaat ctcacgcata
    24421 taccctactc ctcttctcta cataattgta atattgatac atttttgata aacataacac
    24481 tgcctcttca ggagaatcat aaataaccac tttataatta ccgctatcag agtgaattgt
    24541 ttcatcgcct ggcatctttg acaatgaaac aggtaaaatg ggctttttat aacgattcat
    24601 taactctatt aatctgtcaa gaaattcctt ctgatgaata tataataatt ttttaaaatc
    24661 atcaaaaaca cttttttcca tttttcctaa attgtatact gcttctgctg cacgataagc
    24721 aaaaactttt gagccaacaa tgcccagtac tataacggca tcaaaatcct catgagccac
    24781 cataagctca actgcatctt tgaataactg aaaatcaggc tggccgacta aatccacagg
    24841 attgtttctg ctccagaatg gcggcaaccg cttatcaagt tcttccttta ttgattgagg
    24901 caaatgggga ataacaagac ctcgctcagc acattcatcc gcagtaacaa cgccccatcc
    24961 accacctaac gtgatgatgc caattctatt acctttaggc agtggaaacg aatcaaatgc
    25021 tgcagaaagt gttaataatt cacggggcga ttcagctaat actatacccg tttgctgaat
    25081 tacacctttg aaaatggaaa aggagccagc cattgcgcca gtatgtgatg ctgcagcttt
    25141 gctgccaatt tcggttcttc ctgctttgag aacaatgatt ggtttgtgaa gcgatgtttc
    25201 cttagctatc tcaacaaata ctctgccgtc atcaacccct tctacataca taagtatgac
    25261 ttttgtttga ctatcggttt taaaatattt aaggtaatcc tcaattttta atacagcttc
    25321 attacctgaa cccacaaatt tagctattcc aatatcttca ctttctgccc actccataat
    25381 ctgataccct aaattgccac tttgagcaat aattgaaata ccacctggct ttggacgggt
    25441 aatagcccct gtagcatata gagaacagtg agtattgaca atacccattg tatttggccc
    25501 aacaatattt aggccatgat tttttgccag actgataatt tctttttcca tctgagttcc
    25561 ttcagctcca gtttcactga agccagaggt tatcatgact atgtttttaa ttccttttgt
    25621 tatgcaatcc tgcaaaatct gcataacagt ttttgctgga gtagtaataa aaactaagtc
    25681 aactgcttct ggaagctcac gtattgaagg atatgtttta aatccaaaga cttccagtat
    25741 attgggattt accggatagt atcgcccttt aaatttgcca tcaagtatat tgcttgaaat
    25801 atacgtaccc catttaaaag ggatttctga agccccaact acagcaactg attttggatt
    25861 aaacaggcta tcaagcatca tttacctctt ggattattat gtatgtatag cattattggt
    25921 taattagtaa gacatatata tgtatgtcaa cgattttgca ctaatttttt agcaccactt
    25981 ttaatatcct ttgcgcttcc tctgcgattc ccatttccat gctgttgcca caatagaatc
    26041 aatatcagta tacaccggct gccagtgtaa tagttctttt gctttctgtg atgatgcaac
    26101 aagaacagct gggtcgcctg gccttcgggc accttctttt actaaaaaat cttttcctgt
    26161 aacacggcgc acggaatcaa tgacctctct gacactgaac cctttgccgt tgcctaaatt
    26221 aaacacatcg ctttttcctg attctaaaag attttccagt gcaaggatat gggcatcccc
    26281 caaatcattt acatgaatat aatcacggat acaggtacca tcaggagtat tataatctgt
    26341 gccaaagatg gtaacggtat actccggatc aagtacggca cggataacca gtggaatgag
    26401 atgtgtttct ggcacatggc tttcgccaat ctgacctgac tcatctgcac ctgcagcgtt
    26461 aaaatagcgt aaactgattg ccttaaaaga attagatact gaaagatcac gtaaaacttc
    26521 ttcaatgaaa tacttggttt taccatatgg gctttcaggt ttcatgggac tttcttcagg
    26581 tattggaatt atttccggat taccatacac cgctgcagag gatgagaata tgaatcgttt
    26641 tacatttgtc tttatcattg cttcaaagag ggttaatgtt tttgccacat tgttttcgta
    26701 atatttcagt gggtactgaa ctgattctcc tacagcaatg aatgaagcaa aattcataac
    26761 gcatataata ttatagtgcg atagtacatg tgcaagttta tcaaagtcgc caatgtcgcc
    26821 tttaataaat tcaaaacccg aaacaaaatc tttaaatcca ccgcttaaat tatcatatac
    26881 gataggatta aatccttttt ttgcaagcaa tcgtgtgaca tgactaccaa tataacctgc
    26941 accaccggtg actaaaatat ttttcataaa aatatctcca ccaaaaatat taattattca
    27001 cgtttgacac caaaataaac ttttcaatta cctgtcaaca taatgataga acctactatc
    27061 aaacccacta taagattaat acattttatc aataaaaaat cctttcttga ttgtgctaaa
    27121 agtggcagca tggcatggcc atcctgcact atagaacttg ttaccagcac tgaaagtggc
    27181 accatccctt ttgcaaacat cattacgaac aaaagatgtg ggcctgattc gggtattata
    27241 ccaacaagcg cagcaataac aagaacaatc cattgggcat tatgaattaa tgcatctaaa
    27301 tgcaacacat ctaccagcag atgaatgaca atcagcgccg atagtgtcca caaaaatatt
    27361 cttataagat gtttttttat aacatgctcc cataaatgct cttgtaaaaa atgttcaggg
    27421 actatcgcca caataataat ggctatacta ataataactg gcaatgcaat ttttacaaaa
    27481 ccttcctcct ggtggaagaa accagttata accattaata aagcaactag cagcaatgac
    27541 atcagcgaag cccgcgacaa agaaagattt ttccataatg aaataatatt gccaggcgtg
    27601 taatgtgaaa aaggaatttc atcatgaatt accagcccat cacagcaatg ttcaactgga
    27661 ccatgttttt tgaaaatcaa atctgtgcaa tatcctgcca caatgccaat caaagcaagg
    27721 ccaatatgta atgcaaatgc cactgcaggg aaaagagcaa acataacaaa tgcttcatca
    27781 ccactggttg ctatcattgt ggcaacaatt gcacccagtg atattgcacc atgtgcatac
    27841 atagctgcag atgcaaaacc acccagacag cctggaataa tacccagaat aacagcaata
    27901 gtatattgtt ttaatcttgt tgatttaagg ttggcaatct tgccctgtgt aaccacgtta
    27961 atatattcta taagcagcat catcacaata acaaaaacgc taacaaatga tgcttcttta
    28021 ataattgcaa taaaggaatg cataaggatt attaccccct gaaattctat ttatacaatt
    28081 tacattacac tgaataacac gaccatacaa atcttttttt aatttccata taaaaattat
    28141 tgacaatgct ttattagtaa tattgagttt ttaacaatat tttattattt actatcacaa
    28201 aaaagtgaat ttttcatttt tataatggta cgataaaaga agggttatga aactacatat
    28261 cgtaaaacgg ttacttttag taatacttat agctgttact ttgataactt ttattgcatg
    28321 tgaggatgaa ccagttaaaa ttaaacttat ggtcatctca acagtaaaag ggtttactgg
    28381 atattatatc gttaatgggg atacacctgt tcctttctca gctacagaag atgcctatgg
    28441 tattgcactt tttgaaaagg agatagaaga tgtagattat ttagaggtat cagcaacaac
    28501 ctttgatgga gctacatcta tcgaaataaa agtgtacaga gacaacaaaa aagtaaaaag
    28561 tagccagaaa acaatagaag atccttatga cagttataca ttgaattttg aatattctct
    28621 tggtgaagaa gaacaggaaa gttctcaata atattttaaa tattaaggtg aatacatgct
    28681 taactttgaa attcatgatg caacacggga actttcaata gcgcgtgcgt taaaaaaata
    28741 cgaaccccca tttaaggtta ttgataacta tcgcttgatt gacgatggtt ttaatccgga
    28801 agcaacagtc cagattgaag ccaatggcac atttatccat gaagcatcca ctggcaatgg
    28861 cccggttgat gctcttgcaa aagtcttaaa aaaggcgctt ataccactat ttcctgagat
    28921 tgaaaacgtc aatcttattg attacagggc aaatatactt gatgccaaac gaggtaccag
    28981 taccgatgta gaagtaacca tcatattcac cgacggtgaa gaagtgtgga aagtatatgc
    29041 attatcggaa aacatcaatg ctgcatcatt cagggtattg cttgatggtt ttgaatatgc
    29101 aatactgaag aaaaataaaa agaaatcata attattgcaa acttcccccg catacctcaa
    29161 ctaacataaa gtgttattaa ttaagtgcta taattgccat atcatcacac ctcatgtgca
    29221 ggcgcaagcc tgcacaccct aacaaacctc aagtgctata attgctatat catcatcaat
    29281 ggttctgttt tgtgatgttg catctttaag cagtatttgt acggattctt ccaatggcat
    29341 atcttttgtt tttagtatgg attcataaag aatatcaaac cgcaaatctt tttctccatt
    29401 aatatctatc tccaccagac catcggtaaa cataaacaac ctgtcatttt ttgaaagtct
    29461 gatttgattg cttgttatac gtatatcccg ataaacacct aataatgtac cggagctata
    29521 taacattgaa atggtattgt ttgataataa tagtggatag ggatgcccac agcgggtata
    29581 tgttaaatac aattcaccct ttaagtttgt tgttatgact ccataaaacg cggtaaagaa
    29641 gtatcctgac aatatatcta tcagttcctg attaagttta aacataaatt gatcaggaga
    29701 taaaatccta ctattccata aattttctac agcatatttt aaaactgttg aaaataaagc
    29761 agcaggcact ccatgtccgg taatatcagc cataaaaata caaagatttt catcaatagt
    29821 tttcagggtg aaaaaatcgc caccaacata atcgtaggga atatactgtg tatatatctt
    29881 tatcccagga tattgaggca gtgtggtatt tattattgaa tgctggacgc gctttgcata
    29941 ttctaaatcc ttgcttataa tttcatttcg tttgcgcaat tcttcttcac ttttacgcaa
    30001 ggcctcttcc tgctctttct tttcagttat atcttcttta atagcaacaa aatgagttac
    30061 atttcctttt tcatcaacaa caggcgctat tacacagtgt tcccagaaaa agctaccatc
    30121 cttacgctta ttatgaaaaa ttccatccca cacctcacca cgcagtatgg tgtgccacat
    30181 gtgttgataa aattctaaac tttgaaatcc tgacttcaat attcgtggat ttttacccac
    30241 agcttcatca aaagtgtatc ctgtgatagc ttcaaacttt ggattaacat actgaattat
    30301 accatttgtg tcagttatga caatggaagc agggctctgt tctattgctc tgagcaaaac
    30361 tgcctgatca atggaagata atatcttttt catgctaata ttatgttata atagatttat
    30421 ttgttgatat aatatcacgt taatgacaat acatcatttt ttcaatagat atcatcatcg
    30481 ttttcctttt aaaatttaaa aaaatatctt gaccctttct gtcacattct tatgcaatgt
    30541 catcatgaga aagaataacc gtcaacaaat ccttgatatt gcccgcaaac tttttctgga
    30601 gtttggctac aatggcatat ccatacgaaa tattgccaaa cgggcaaaac tgacaacagg
    30661 ggcaatctat ttccatttta aaaataaaaa gcagatttat agcaccattt gccttgaggc
    30721 aattgatacc ctgcacaaac tgttctgtga aggcatggca gcacgtaaaa ctcctgcaca
    30781 gaaacttatc tcaacgtatg actcatatct taaattttac tatgaatatc atgattacta
    30841 taacatatta accgaataca aagctgaata cgatgatgaa cctgacccac ataaagatga
    30901 aatccagaaa aagaatgtag ctttacttga attgatggca ggtgcgttta ctgacggtgt
    30961 aaaaagaggt gactttaaga atttagaccc caagatggtt gccttttttc tttctgcagt
    31021 tgcagaaggc atgcttcagt ttaaaaaatt aggtgtcatg gatgctgcag gtatcagcga
    31081 caaaaacttc agggagttca tgaagattat cgttggtcag ggtatacaaa aataggactg
    31141 taacattttc tttactcaac gtttaataaa ttcttttctt acacttttac tcatcattat
    31201 ttcatacaat cgtggaaaat attttgctat taaaaaactg agtttcccca ctgttgttag
    31261 tactagcatt cttttgcgct ttataacccc tttataaatt gcttgtgcaa cactctgtgg
    31321 acttgcttcc ctgccaacta tagccctatc taaagtattg atagttccat ttccagaaag
    31381 agcccgtgat tgcaaattag tacgagtaaa tccaggacat accatcataa tacaaacacc
    31441 atcatccttt agctcggctc gcaagctttc aaagaatccg tgaagagcgt gtttgctggc
    31501 actgtatcct gtccttccat ataatggcgc tataccagca atacttgaaa taacaacaat
    31561 aacacccttg cgttcaataa tgctgtttat agctgccctg gtacaataca gggcgccaaa
    31621 gaaattaaca tccataactt ttttatacac cgcagcgttt gtatcaataa aaaggctacg
    31681 ctgagttatg cccgcgttgt taaccagtat atcaatacca ccgtattgtt ttattatttt
    31741 ggcgatagtt accgtgcatt gtttttcatt ggtaatgtca cactgaaaac caatcacagg
    31801 tatattatta cgttgcaaaa aattaacctg attcttcaat gcaattgtat ccacatcaag
    31861 aattgctatc gatgaaccgt gtaatccaaa ttccagcgct atggcagcac caataccgct
    31921 tgcaccgcca gtgataacgg tgactttccc ctgcaaatta ttcatgacaa acctttttta
    31981 tttttataca aactttcgca catgatacaa taatatacat tacaacgttt tatatacgtt
    32041 ggttttatat gcttcctcaa gtgcttgtaa cggagcttca cgttcccaca atatcaaggt
    32101 ctgatttttt gcaggacctc ttttaacagg atcacgacca ttcatacagg cctgtatagc
    32161 atttttaaat tgtgaaatca atgcttcagc actttccata ctatttgaat tgttaaccga
    32221 ctttgatatt actttcacaa catggtcctt gaagcgtggc acagcaaaga gctcatccat
    32281 atcaatacca tagaagtatg caataaaacg atacgcattt tccacctcct cacgatcatc
    32341 ttcagattgt gtcaataacc aacctgctat tgcattgcaa agaagtgtca cgctggtatt
    32401 gagtagtaaa atatcccacc agttttgata tatcgcaatt tcactttcaa gatttgcata
    32461 atattctaat tcagctattg acatcacgtt gggaattttt gcttccatct tttcatagta
    32521 gttgaccata acaggattgg aatttctttg ggcttcttcc ttttttccct ccaattgttc
    32581 aagttgctgc attatgctca acttcaaaaa gattttttca tctgccattg tgcgtaaaaa
    32641 cgttagggca ttctgagatt gtttctcaat ttcaaaaatg cgctcatata ctgaacgtgt
    32701 tttcttcatt tccataggaa gctgttcata ggcaatatga taagcagcag caacctgatc
    32761 cacagaaggt attgttgcca gagatgtatc ttgtttttcc tgcgcaagtt caacaagtaa
    32821 cgcactcata tcagccattg atgtgctaac ttctttatca gaataaaaag tcattgcatc
    32881 gtttagcaat tcagcctttt tacaaagctt tgcatatata tcatatgctt tcatcagttt
    32941 agcatgtgac tcacctaact tttgcttata tgctgccaga gctgtctggt aacctgatag
    33001 catctgttta attgtatcat ccattattgt aacacctcac caagccatga aagaattagt
    33061 gaaagtcttt cattatcaaa cggaataacc cggcgatatg gtttatgcat catgatttta
    33121 tcaacagtga tttcaggatc acattgctgc agttcattcc agatagctac aacttcagct
    33181 tttcttttct gttttctgct tttacaggat acatggtatt ccacacatcg tgttataagt
    33241 tttacaatta attctaatgc tctatgagta ttttgtataa gtataccagc atcgtccttt
    33301 gctcttgaaa tattactata aagctctgta tggtttgaag atgcctgcac cgcatcaatc
    33361 attatttgct cagctttcac accctcccta tcaccaaaaa gcgttttaat gcgtttcaaa
    33421 gattcatatc tatcacgttg ttcagccctg gcaattaaaa acatttcgcc tattgcttca
    33481 gcaactttgc catagtcatc atcgtgtatt gcatccaatt tttccaaata tgctgtatag
    33541 gcagctattt gatgtttatt taattcctga ttggtcttgc attcattaag ccttgtttca
    33601 aaatatcctt ttaactcttc aagtatattc ataactcatc tttcattact aaattttaat
    33661 tttagaaaat aatattttaa cattactctt cacgcattgt ttaaaaagta agtttataca
    33721 aagatttata aattaggcat gtttcttttt taaaataaga aaacgctgta tctcagttat
    33781 ggagatagct acaacaaaca tgccagcaaa cacaagaatt gtttttatta aactccactg
    33841 ccatagaacc ggagcactaa cccagagcat ccctaaaata atatatggag aggcaaacat
    33901 gagcataaca cctgtttcaa gcgctacggt tgttttaaat tcaggatcaa ctatacgcaa
    33961 aagcaacaat ccggatgaca ctgttcctgt tacagttcca tatatggcaa gcatgcgttc
    34021 taatttaaaa ctccacaaac gtttgccata atacacacag aataacgttg taagtaaacc
    34081 acctgcaata ctaataacca gaataggaat aatgtattgc cacacaacca caacctgaat
    34141 tgccatgatg gtggcaacaa gaagataatc aacagcaaac ccggtaatac gccgctgaat
    34201 accagggtca atgagatata atcctcccat ttttgaaatg ataagccgta caataaaagc
    34261 tatgacaagc ccaatgaaga agaaaaatcc ccaaaccatc tgacccattt caggacttaa
    34321 tacactggct actttataaa caacagcgta ggtaatcatg tacaccagcc ccatgatgct
    34381 tgcctgaaat gccattgcat caacattcga agaatgtgtg gtaagctcac cggctatttc
    34441 tttttcactc tgaggttgta ttataccttt acggatttca tcagataatt cttttggagt
    34501 atgtactgcc ataccttttt tgatacccca gtttgccaga ggcactccaa caaaaaaagc
    34561 aaacaggaat ccaaatacag caaaagtaag acccaatgta gctgaatgtg catatcctaa
    34621 attttcccaa accttgccaa ccgataatgc ctgccctggc ccttcagtaa aacctaacgg
    34681 tgtaaaaaag ccaaaagcag ggaaaaggtt aaatcctata gaatttaaaa gcataacaca
    34741 taatccacca gcaattgcct gtattgcaaa tgatatgcct tcaacatttg ccatccatat
    34801 tgaaccctgc aaaacttcac cagcacgctt caaatccgct ttttctttat tgtacgttag
    34861 cccaatagag ataaatgata tgttaaacag gtggaaagct aacgtttcca gaatatcagc
    34921 agtaataggt ataatctcat aacttcgtaa aacaagccct attgcaccac caataagaca
    34981 acttgggata agaaaccttt gaaatgcttt aatatttgca cgcagaataa tgcctaaaag
    35041 tagcattatt gacatccatc caaaagcaag catagcatca aatgtaaaag gcagtttcat
    35101 ggcatccccc atttttttaa tgtatatttt tatctatagg ctctgacccc cttgctgtga
    35161 tatgattata taatcatatt taaaacgatg tcaattaata atttattttt aatgtaatgt
    35221 aaacagtgat tccacacatc aaactctttg gcatctataa aaattccatc tggtaatact
    35281 aaaaatcata aagcacagac atagacaaac ctttatatat agtaaaaaaa tgtttgcatt
    35341 aaccacattt tttcactaca ttcaataacc actaaacact acatattgca tctggaggta
    35401 gccatgaaaa taattgatct ttcatcaacc attgaacctt caccaccaca aactcccgat
    35461 tttttaaaga ttgatgtaca gtatcactcc catgctgatg gggcaaaaca ggtggaagca
    35521 cttctgaaag tgcctgcaca tcttatgcgt aataatgaag gctgggcaac cgaaaccatt
    35581 acccgccttg gcacacacga tagcacccat gttgatgcac cctggcatta taacaccacc
    35641 attggaggaa aacctgcacc aactattgat gaattgccac tggaatggtt ttatggcaat
    35701 ggtgttaaat tagatatgac ccataagcct gaaggcaatc ccgtaacacc tgaggatatt
    35761 gaaaatgaat tacagcgcat caagtattca ataaagccca atgatatagt cctgatacaa
    35821 actggcatgg ataagtatta cggacagcct gattatatct tccgtggttg cggcgttacg
    35881 tatgaagcaa cagtaatgtt atacaatttt ggtgttcgtg tgatgggaat tgatgcgtgg
    35941 ggctgggata tgccactcaa tctgcaagca cagaaggcac ttgaagcaaa taagcctggc
    36001 atattctggg ctgcccatca ggctgacctt gtatactcgc atatggaacg gcttgtcaat
    36061 ttagataaag tgccttcaac aggattcaag gttgcctgtt ttccgctaaa gattaaacgg
    36121 gcaagtgccg caccagcacg cgtggttgcc atcattgaat aactgctttc atcaagctgg
    36181 cacaacacat attctgaagg tgtaaaacgg acttcgtgca gtggggcaac gtctgaatcg
    36241 gtactcccgt gctctttgca atggactgct aaaatatgag ccaccacgca gtaccttata
    36301 agtggtacca tattctttat gtggcaggca accaggatat ccttgaaagt aactatcggt
    36361 ccattcccaa acagtaccgg gcaacacctg gcgttgtttt gtaagctcaa taaggctgtg
    36421 gcagtagtcg ctaagcagta catgtttatt gggcgatagt tcctgcaatt gttccatacc
    36481 tggtatacca acctttgctg caaaagcctc ccacataaac tccgtgggaa gcataatcac
    36541 ctcccccgtc ttttttgaca gatactcaca gtatgcccgg gcatcattgc agcttacctg
    36601 caacacacac atactgctac tgtgttcagt atacagtgta tcaagttcat caatgccaaa
    36661 aggcttttgc caccacaggc cttgtttctt agtccagccc ttttcccatg tccatgacca
    36721 cccatccttt tgcgcctgcg taataaaacc ggtttcctca ataaactgag caaattcact
    36781 cacggtcaca tagcgcattg ctatggcaaa ctgttgcaat gctaccgtgt gttgcggaaa
    36841 tgaatggtac aggtattcag gttttatatc aggtggcaga taaggtaaca atgtctcaat
    36901 agaattttgt gtaataccaa tggtatacaa gccgtcagga atatgatagc aaaaagagga
    36961 acacattagc aatctctatt ccttatgttt agtttcaata attgttacaa attcatcagg
    37021 tgtacaggca ataatattcc ctgatgaata atcctttgta tttctggtta tgatatattt
    37081 tacaccattt ttcaacgcag tataatattg tatagcatct tcaaaatctt taaactttga
    37141 ttctaatgcc agatttataa tctcatcatc tacagccaga acatcaataa ttaaacgcaa
    37201 ttttttaata aattctacgg ctatatcatg tgattccaat tttctaagaa tgtagtaaag
    37261 atttgttata attactgttg aggtgaaacc atggaattct tttaattcaa tgaggcttaa
    37321 aacaagtgct gaattgatat aataaggatg acgtctcagc gcaacatcca aaataacatc
    37381 tgtatcaata aataatttat tcttcatgca ctgatgattg atttaaattt ttgtttgatt
    37441 ataaaaaaac gcatgcgtct ttaactatca aaatatacat ttttttaaag acattgttat
    37501 tgaaaaaaat agtgctttta ttacaaatca tatttgaaaa gcaaataatc ctcaatttca
    37561 cgcttataat taattttttt tggtttcttt actataatat tagataattc ttttgtaata
    37621 ggtgctattg tatatttatt atggttatca accaatgatt ctaaaaattt ttcaactacc
    37681 tctgaaatac tggttttatt tttacgcgca tactgttttg cttttgttat cactttctta
    37741 tcaatgtata atgttaattt tgaattcata gttcaaacct tataaatatc tacgtaataa
    37801 aatataaaaa caatacgtat atgtcaagta ataatttaat tactacacca ttgcatctat
    37861 ttcttcttct attgccttta tagcttcaga agctttcagt atttttttct tttcacctct
    37921 tgcaaacagt acaaccctgc catcacgtgc tccagcaatg ccaatatctg catgccgtgc
    37981 ttcaccgggg ccattaacct cacatcccat taccgctatc gttatgtgtt tgtgcttttc
    38041 aatcaatgtc ttttcaaaat gtaattttac cattgattct acttcctgcg tcaattgcag
    38101 tatatctaat tttgtatcgg tgcggccaca tgttgggcag gcaataatgg tatatggcct
    38161 gaatcggaaa ccgccaattt caaggatgcg ctttgcagct cttacctcat ggacaggatc
    38221 acctgtcatg gaaagtcgta tggtatcgcc aataccttca tatagtagtg tgccaataac
    38281 catactgctt gcaataatac aatcagtgcc atagccagct tcggttaatc ctatatggat
    38341 gggatactgg cgctgtgatg caaagatgcg gtttgcttca attgtctgca gcaaatccga
    38401 tgattttatg gaaacaacta tactggtaaa cctgcaatct tcaagaagct gcacatgctc
    38461 catggctgaa gcaacaagcg tttggggatt tacttcccca tattttttta tatctacaga
    38521 acctgagtta acaccaatcc tgatagggat atttctttca ctggcagcct tcactaaagt
    38581 aaccacattc tcttttgcgc caatattgcc aggattaatg cgcaatttat ctatgccgtt
    38641 ctctatggct ttcagtgcta ttttgtaatt gaaatggata tcagcgcaca atggtacggt
    38701 tacttctttt tttatttctt ttatatactg tgcatcatcc tctgtacgca atgcaagccg
    38761 tacaagctga gccccttcct gctgcaaacg tattatctgc tcaattgttg cttttacctg
    38821 gcttacaggt atatttgtca ttgactgcag cgatatcggt gcatcaccac caatggttaa
    38881 actgcctgcc tgtacaggaa tggtttttct gcgggaataa ttcattaatc agtaactatc
    38941 gcacattaaa ataaatcttt gaaaaatggc aaaaacgata aatcattgaa tataacaaat
    39001 atacccaaaa gcaccagaat ggcaaaacca atcatttgaa ttttttctaa tagcttctgg
    39061 ctgaatgcac ggcgcgtaat ggcttcgtac agaaagatga gaataaagct tccatcaacc
    39121 atgggaatgg gaagcaggtt catgaccata aggatgatgg aaatttttgc catgagtatg
    39181 ataaatgcag acacgccgcg atagtatgca gtatcgccag caatctttgc tatgcgtatt
    39241 ggacctgata aattttgccg cacatcaagc tcacccgaaa acatcatgcc aatgccgcgg
    39301 aaattaagtg caataaaatc aaatggatca accagcgatt ttacaaaagc atcacgtaca
    39361 ccatactgta taaatttatt ttcaggtgaa atagcagttt ctatacccac aaatccatac
    39421 cgttcaaatt tcatgatgcc actataggtg ccaccaggag tcttaagtgt tatgacacct
    39481 tttgaagcat taataatgtt ttgcatttct tcaaatgact ttactgcttt tccatttatg
    39541 agaacctttc cagcatttgc tgctgcttta atcagcgaca ttgaatcaac cgtaattgta
    39601 tagctttctt ttggaaagcg catgtctgta aactggttta tcgtcaacac ctcttttgtt
    39661 tgtggagtta tgggaagtgt aatgaggtgc ccacctctac ttatggtaag cgataattcc
    39721 ttatcaggat gtcgccgtat aaattgtgtg aattcctcag gtgagtggaa ctgagtccca
    39781 tttgcttcaa tgatctcatc catttccttt aaaccagctt tttttgccgg ggaattatca
    39841 agaataccca taaccagcac cctatcgccg tagggcataa caccaatggt atagtatccc
    39901 ttctttgtgt atttttttgg ggcgatagtt acttccaatg tatcattgcc cctgattgct
    39961 ttaaccgtga taggtttgcc tttggaaaag gctactttgc tctgaatatc ggtaaaccct
    40021 acaatctgtt cattgttaat agcaacgatg gtatccccag tcctgagccc tgcttcatac
    40081 gcaggcgaaa catattcacc ttttgttaaa tattcaggga tatagatctt atttgtttca
    40141 acaggataac cgtggatatt catgataaag aaaagtatga taccaaaaaa caggttaaac
    40201 agtggcccca tgagtaccgt aacaacacgc tttaagggat gtgcacttaa aaattcatac
    40261 ggattgcctt gacgctcttc gtttggatcc tcaccataaa atttacaata tccaccaaat
    40321 gggattaatg ttatctgata ggttgtatcg ccccattttt tctttataaa acctttacca
    40381 taaccaatgg aaaaaacttc tgcctttatg cccaccattt tgccacctaa caggtgtccc
    40441 aactcatgaa caaaaataca caaacccagt aaaattacag ctgctaaaat atacgtaagt
    40501 gttaccatta tttacctcat atacatgtta gtgctaattg tcgtgcttct gcatcagcat
    40561 tgaatattgt ttgcaaatca ggatcatcaa tcttcctatg cttccctaaa accttttcaa
    40621 ctactgatac tatgttagta aacgaaatct tcttctgtaa aaacgcattg acggctacct
    40681 catttgctgc attgagtact gccggcatgg taccaccctg ccttccagct tcataacaca
    40741 gctcaagtgc cggatagcgc tttttatcag gttttgtaaa atgcagggaa cctattgctg
    40801 ttaaatcaac gttcccaaaa tcattatggc acacctgtgg atacgtcaat gcccgctgga
    40861 taggatacac catatcggta atgctcatat gagcaagcaa tgtgccatca atgagttcca
    40921 ccataccatg aataatgctt tccggatgaa tcatcacctc aattgactca tacggcactg
    40981 aaaaaaggta atgtgcctct ataacctcaa gccccttgtt catcaatgtt gcagaatcta
    41041 tggtaatctt tttacccata tcccatgttg ggtgcgtaag ggcttcttcc acagtaacag
    41101 tatcaagcgc ctcaaccggt ttatgacgca cacttccacc gcttgctgtc agtattaacc
    41161 gctttactga atcagtattt ctgccaccaa gcaatgtaaa tatagcacag tgttcactgt
    41221 ccacaggcaa aagttcaaca gcataccgct gtaaaagctt taaaaaaata tcacctgcca
    41281 tcaccagtgt ctctttattg gcaattgcta tgcgtttaat atactgtaac gaatcaagtg
    41341 ttggcttgag tgcagcagca ccaacaatgc caacaaccag aatatcagca ccatttgcag
    41401 caagcttgct taagccttca agcccttcat aaaaaactat cgaaccaaat tcttttttta
    41461 actttttata ttcatcagta gaacacgttt cttcatcact tacagcaacg tattgtggat
    41521 gaaattcaag tatctgctct tttaatagtg ttatattttt atggcacgat agccctttta
    41581 cagtaaatct gtttccaaac tgacgaatca ctttaagagt agaaaggcct attgaaccgg
    41641 tggaaccaaa tattattacg cttgaatgca taaaccaatg agtaccttat ataatccctc
    41701 ggattaccag atagtagtaa aataatggca ttgcaaatat cgttgaatca aacacatccc
    41761 acataccacc atgacctgga ataatagaac ctgaatcttt tatggcacca tcacgtttta
    41821 ttgctgattc aaacaaatca ccaatattac ctataatact gaatataata ccaataatgg
    41881 ctgattcaac aatactgaag aaatatctac catacaatac ttcaattagc tggctgtaaa
    41941 taattgcaaa aacaatgcca aacaaaagtc cactgaaata cccttcccat gacttattgg
    42001 gcgatgcttt aaaattggct ttatgcttcc caaaatacac acctccaaaa tatgcaccgg
    42061 tatcattaat cataacaatc agatgtacaa aaataatgaa tagaacacca tcacgtaatg
    42121 atttcaaaaa cattatatgt gcatacggaa taacaatgat aaaccagcca agtatagttg
    42181 cacctaatga gtatatcccc ccttttaatg ggcgctttac aatatgaatt gtagcaaaca
    42241 cactgatagc tatcattaca gctgcaagca gatagcgtac atcaaaattt actgttaaac
    42301 tgccaatacc ggtaatgttt ccatatgcat acgaatacat gataatattg ataatcaatg
    42361 ctgaaatcat tcccgtcttt acaaatggcc tgtaaccggt ttgcccttca gctatctgat
    42421 aaaattccca caggcaaaca ccggtaacaa tgatgctaac cagaagcacc ggcaatgaat
    42481 ataatgagtc cgttacaatc aaaaacacat aaaccggcaa tgcaacaact gcagtcagaa
    42541 tccgtattaa agtattttta gtgcgctctt tcataagcca ccaaacctcc tttccctgtg
    42601 ttgatattcg tatattgcct ggtagagatg tttctcatta aaatctggcc acaatacctt
    42661 cataaatact aattccgcat atgctatctg ccataataaa aaattgctta tcctgtattc
    42721 accactggtt cggattaaaa gatcaacatc cggtaatcct ctggtatata aatagccttc
    42781 aaatatttca ggggtaagcg atagctccgg ggaaggtttt ttctgtagcc attcattcac
    42841 cgcatgaata atctcatgtc ttccgccata attcaggcaa aagttaagta ttatcctgga
    42901 attgtttttt gatttttcaa cagcatcagt aataatgcgc tgaacattta caggaagctc
    42961 cttaagactg ccagaatgcc gtacctgaac ccctttttca attatggtgt tcagcttttt
    43021 ttcaaagaaa gattccagaa gattccacag gccattaatt tcttcttttg ggcgtgacca
    43081 gttttcagtt gaaaacgcat acagtgaaac aacattaatg ccaagctcta ttgcagtatc
    43141 catgcagcgc tcaatggttt cggcaccacg cctgtgtccc tcaatacgcg gcaagcccct
    43201 tctttttgcc catcttccgt tgccatccat gatgatagca acgtgccgtg gtatattgga
    43261 gtaatcaatc tttcgtttat aatgtataag catattaata agtatactaa tgggcttgaa
    43321 aagttacaag ctcattatct ctttttcttt gccgtcagtc attgtctgta cttcagctac
    43381 aaatttatca gtgatttttt gaatttcatc cagtgcattt tttgcgtcat cttctgatat
    43441 ttccttggac ttttcaaggt cttttaccat gtcattgccg tcacggcgaa tattacgtat
    43501 agcaactttg cattcctctg ccttttgctt tatcatctta acatattcct tgcgccgttc
    43561 ttcggtaagt tcaggtattt gaatacgaat gatattgcca tcattatttg gggttaaaga
    43621 aagattggat tttaatattg ctttttctat agcacttaaa ttggttctgt cccatggctg
    43681 tattactaca agccgtggct cggggcagga tattgtagca acctgattta atggcatttc
    43741 ggtaccatag gcttctacct taacattatc aaagattgct ggatttgccc tgccagtacg
    43801 gattgttgca aaatcctttg ccagattttg aacacttttc tgcatgcggt ttttcacatc
    43861 atcaagaata tcttttatca tatattcccc caaccaaatt aatattacga aatccgtgtg
    43921 cctatatctt caccttttaa aatctttacc agtgatcctt catcaaacaa attaaataca
    43981 acaagtggta gtttgttttc catacataat gacactgcag tgtaatccat tacccctaat
    44041 tgttgctgta aaacatccat gtatgatgct gcatttattt tatgcgcttc agggaattta
    44101 accgggtctt tgtcgtaaat cccatcaact ttggtagctt tgaggaaaag gtctgcacca
    44161 atttcaatgg cacgcaatgc agcagcagta tcggtagtaa aaaacggatt tcctgtacca
    44221 gcagcaaaga taataattct tcgcttttcc aggtgacgca atgcccgcct gcgtatatat
    44281 ggttctgcaa cctggcgcat ttcaatagca gtcatcacac gtgttggtat tttcatgttt
    44341 tcaagaacgc tctgaacagc aagcgcatta atcactgttc caagcattcc catgtaatcg
    44401 ccggttgccc gctcaatgcc aatggatgtc cccattgacc ctctgaagat attaccacca
    44461 ccaatgacaa gtgcaacatc aactcccaat tcgtaggctc tcttcacttc acgggcaatg
    44521 tgggaaacaa tctcaggatt gattcccacc ttttgctgcc cggcaagtgc ttcgccactt
    44581 aatttaagta aaattcgttt gtattgtgct ggtgactctg attccatcct gtttaacctt
    44641 aatcagtaac tatcgctgcc caatctgaaa gcgtacaaaa cgaccaacgg tgatgttttc
    44701 accatacgtt gctatcttgt ttttaatgag gtctttgatc aataccttat tgtccttgat
    44761 aaattcctgc tcaagcaagc atacttcaga ataaaacttg gtaagttttc cttcaactat
    44821 tttttcaatt acatttgcag gtttaccact ttcttttaac tgctcacgat aaatctcttt
    44881 ttcacgctcc aggacttcag ccggtacatc ctcaggactc acatacagcg gatttgctgc
    44941 tgcaacctgc atgcacaggt cctttgctaa atcctgaaaa tcctggtttc gtgcaacaaa
    45001 gtcagtttca cagttaagct ctaaaagtac gccaagctta ctgttattat ggatatagga
    45061 agctatgata ccttccttgg caacacgccc ctcacgcttg tttgcagcag ccagcccctt
    45121 tttgcgcaga taatcaactg ctttttccat atcgccgcca cattcaatta atgctttttt
    45181 acaatcaagc atacctgcct gagttttttc acgtaactct tttatcattt cacttgtaat
    45241 ttctgccact gtacaactcc tttatttaca taaaaattaa ttatcataca aaatacttaa
    45301 cgcctgtcat cttcatattc atcaaattcg tcagcatact cttcagcata atctttttca
    45361 ggtttagctt cagctgcttc ttcttcaaca aagtcaattt ttgcttctac ggcttcaccg
    45421 ctcacctgac ctgctgactg accttcaatg atggcattag ccataacttc cagaaacagt
    45481 gctatagccc gtatagcatc atcattgccc ggaatgggat aatctatttc atcgggatta
    45541 caattggtat caaccactgc aaaaatggga atacctaatt tacgcgcttc ctgaacagca
    45601 atagcttccc gctttgggtc aatgataaac aaagcatcgg gcagcctatc catatctttg
    45661 ataccttcaa gtaccttgcg cttgcgttca agctcacggg taagctccaa aatttccttt
    45721 ttggtttcgg cttcccattt tccggtagct tccatctgtt caagttcttt caatcgctgt
    45781 atagaccgcg aaacagtttt ataattagta agcaatccac ccaaccagcg ctctgataca
    45841 tagaactgtc cacacttctg agcatattct ttcactgcac cctgtgcctg tttcttggta
    45901 ccaacaaaaa gcaccctgcc accttttgca gcaatttcct tcattgcagc ataggcaatt
    45961 ttaagccgct gcatggtttt ctgcaaatcg atgatatgta taccattacg cgcagtaaag
    46021 ataaactgtg ccatacgagg gttccatcgg cgtgtctggt gcccaaaatg aaccccggat
    46081 tccaacaaag ctttcattga tattactgac aatgaaatac ctcctgataa tataatattc
    46141 aatgcttacc aaagcatcat gtatactcat caaataaaca catacagtgt tatttgaatt
    46201 atgtacagtt tgggcgatag ttcatagtaa aaaacttctg gtatgtggca accggctact
    46261 gtaaaccggt ttacatattt acactccgct tttacggaat gcgtgatagt tgcaggagca
    46321 acctttacta cttaaatcca tttgaacaat aaaacacctg caatcattcc cacaattgca
    46381 gaaatattaa aacgaatgta aaaacttatc aaattcagat caaatccaat tggcctggtg
    46441 agaggcgcat tgataacagc cagcgaagga gccagcttaa ctataaaaga accaattgca
    46501 attcctaata taccaccaag aattacaaat cccaaaaaat accctatatg atcgcgtgaa
    46561 aatgtcataa tacttaactt ataaatattg taaagcaata gaattatact agtaaaaact
    46621 gtcaagataa ttttaactat tattgctttt tcatctcttc attaactttt tctgctaaaa
    46681 atagcttaat tagagattga tagggaatat ccttcttatt tgctatattt tttaatttat
    46741 atatcaaaga ttctggcaaa cgaattgaaa ttgatctggt cgttggttta atatttggca
    46801 aatacatttt ttttgattta gcaaaattaa tatattcagt tgcatcatta ttgctccaga
    46861 attctctttc tttatcttcg ttatcaaatt gtggtatctt ttttaagttt ttcatatatt
    46921 ttcctctcat tcttattcat gtcacgtgca gatattaccc gtattaatgt tttgcgtatt
    46981 gtgaacacaa taaataaatg tcttttttga tcagtctgac ccagcacaaa atatctatct
    47041 tccttcaaag aatgtttttc atcaacatag gtaaaaaatg gttcattaaa aaatacttct
    47101 tcacattcag ctacagacac tttatgcttt acccaatttt taaattcatt gccttcgtcc
    47161 caatcaaaac ctgttatatt ttcaaaatca atttgtctca tactgtatat gtatagtata
    47221 tacaatcatt atatctgtca aataaatttt ttattattgc attttttcaa taatacattt
    47281 attcgataat ttaatataat tgtactgaga tttcaccagt aactatcgac aaaagaatgg
    47341 tataaacttt taatttacag ctattcatga atcagcgtta ccgttaagct tttggatgtg
    47401 atctatatta ctcccattta cagcttgaca tttaatggaa atattattgc attatgatga
    47461 gaattcacta aacactgtgc ttcgttgatt gacacaaagt gtttcaatat gctaaaaaat
    47521 cttttaacat ataatcctgt ttttctggca cttgttgcta ccctttttac ctgggctgta
    47581 actgctttag gtgccgctat ggtatttttc ttttcttcaa ttaacaagaa aatacttaat
    47641 tcaatgttag gatttgcagc tggtgtgatg atagctgcaa gtttctggtc gttactcaat
    47701 cctgcaattg aaatggcaca atcaacaggc aatactccat ggattcccgc agtatcggga
    47761 tttttatgtg gtgctgcatt tttactggtt attgacagga tattacccca tctccacatg
    47821 ggacttgcaa tagaaaaagc cgaaggagtt aaaacttctt ggcaacgaag tgttctttta
    47881 gttcttgcta tcaccatgca caatattcct gaaggtcttg cagtaggtat ttcttttggt
    47941 gcactgacta attccactga tacaggtgta ttagcaggtg ctatcgcttt agcaattggt
    48001 attggactac aaaacttccc tgagggcgct gctgtttcaa tccccctgcg gcgtgaaggt
    48061 ttttcgcgtt ttaaggcttt ccaatgcggt cagttatccg gtattgttga accaattgca
    48121 ggtgtgattg gtgcatacct ggtttcagta attacaccac tcctacccta tgcgttgtca
    48181 tttgctgcag gtgccatgat tttcgtggta gttgaagaac ttatccctga atcacaaacc
    48241 ggtaatgaaa ccgacctttc aaccattggt gctatcattg gctttgccac catgatgctc
    48301 atggatgttg cattagggta atgtgcaaat aaaactccac taaccaatta tcttatagct
    48361 ctcatattgt tgattatcct tccatctttt aaatttaaaa ctaacggttt tatatttttt
    48421 aacaatcttt atgttgacta ttgttatcat gttcagtata tttttatact tattacaatt
    48481 aaggagaact aaccatgacc acggtcaatg aaatacttac atctcaaaga aaattttttg
    48541 aaacaggtgc cacaaaagat atacatttca gattacaaca acttgctacg ttacgcaaat
    48601 cactgaaaac atatggtaac cgtatcatag aggctctgga taaagacttt aaaaaaccta
    48661 aatacgaaac aattgccacc gaaatagcag taaccatata tgaaattgac catgccataa
    48721 agcacctgaa gcaatgggca aagccaaaac gggtaaaaac tccaatagtt cacctttttt
    48781 ctaaaagtta tattttcccg gaaccatatg gtgttgcact tattattggc gcatggaatt
    48841 atcctattaa cttagtattg aatcccttaa ttggtgcaat agctgctgga aattgtgcta
    48901 ttattaaacc agcctatacc gcacaggaaa gttcaaaagt tttgtatgat atgattaatg
    48961 agtttttccc tgaagaatat attgctgtgg ttcaaaaata tcctggagta tatgacgaaa
    49021 ttttgaatca aaaatttgat tacatcttct ttaccggaag tactgatgtt gggaagacta
    49081 ttatgcaggc agcagcaaag caattaactc ctgttacgtt agagctggga ggcaaaagcc
    49141 ctgccatagt atgtgatgat gccgatattg atgcttcagc ccgacgtatt atgtggggta
    49201 agtttattaa tgctggccaa acctgtattg ctcctgatta cgtattgatt cataaaaaca
    49261 aaaaagaaga atttatacat gcatcacaaa aatatataac tgaattttat ggggatgatc
    49321 cacaggcaag taatgacttt gcgcgtataa ttgacaatca gcaatatgat cgtcttgcac
    49381 ggatgcttga acactgtacc attgccgtag gtggccaaac taacaaagaa gaacgataca
    49441 tagctccaac acttatcacc aatattacac ttaaccatcc atcaatgcag gaggaaatat
    49501 ttggccccat actccctgta ctggagttta ccactattga agaagcagca cagattatta
    49561 aatcgtttcc aaaaccactg gctgcatata tcttcacaaa taataaaaga acctgggaaa
    49621 agcttattga tgatatttct tttggtggcg gttgcgttaa tgatactatc tcacactatg
    49681 tttcgccgta tttgccgttt ggtgggattg gtttcagcgg gattggacaa taccatggaa
    49741 aagcaagttt tgatacattt acccattata aaggcgtttt aaaaaagccg ttttttatag
    49801 atacattttt acgataccca ccttataaca aaggtaaggt atcatttata aaacgctatt
    49861 tcagagttaa gctgtaattt ttatttgaca ctattggaaa atttatctaa tatgtaatta
    49921 aataattatt ccaggtgcaa aaggatatta tacatgggtt tagctttaaa aacatatgag
    49981 atatttaaag aaaacgaagc tattgcaaaa ggcttagcgg aaattattga ttcgctggaa
    50041 cagtctataa aacaaaatga atcggcaaca aagcaggatg taagggaaac tgaattgcgt
    50101 ttaattaaag aaattgaaca aaatcgattt cagatgaaag aaattgaatt atctctttta
    50161 aaagaaattg aaaccgttcg ttcaagtaca attaaatgga taattggtct atggtttact
    50221 caaactattg caatagctgc atttatttat accgttatag tacaaattaa ataaaaacgt
    50281 atggcaacac aaaagaaaaa aaacaataaa agtaaatcgc aggctaaaaa aacacaaccc
    50341 ggatcgaaat caactgctac cgctgctaca gcacaataca ttaaagctat taacctgctt
    50401 ttaaaaaaac ttgatgtgga aagtcttaac tttctttatg aacaggccaa ggtgctttta
    50461 cataacctgg aagttaaaaa attgcaacat gaatttgatg aacttgacgt taaaaccatt
    50521 actgcaaaac aaaaaggata cagcaaagac aagcttgaag tagttgaggc tgatgacctg
    50581 cgccatttta tttttgttat caatggtgca cgtaatttct tcagccgtga tgaaatgcga
    50641 aaaattgtta cactatgtca cagcgcgcct acagtaaaag atggcatgcg catgttatat
    50701 cactggtttg aaacaaatag aaaagacgtg ataattgata cagctattga tgggtcacat
    50761 gacaaggcac ttgaaacaat atataatcat attataaata attatactgt gaattaacaa
    50821 aatagtaata gaatacttca cgccattgaa ggcgggttat aattcttttt acaatgaata
    50881 aagacaacat cattaatatt aatactattg ataagacaca gcgttacatt gttgtgccac
    50941 gccgtattag aggtaaagta cagaaagtag ttaaaccatt aaaaaaagtt aagggcaact
    51001 gttactttac ctgcaatatg ccccatgccc ttattaattt tatttacagg agtgtaaaaa
    51061 aattaggact taccagttca aatcttattt tttcccgtgg aacggttcgc attaataata
    51121 gagttgtcca taatgcggtg tcatcaacag tacttgactg ggatttagga atttcattta
    51181 tcataccact taaactacgg tacaatacat ttgttacaat tgaagtggat aataatgatt
    51241 atagcgtacg cttaatggaa cttgtcattc ttattgcatt gatggctcat gcacatccat
    51301 caaagactcg aaaaaaacat atagaaaatg cacataaagt actggcgctt ggctggaaaa
    51361 gtatactcac atagattacc gttcaatttt ttctggaagt cggtacatat atttctttcg
    51421 aaaaataacc tcggttacaa atttatcaac tctgcgtgca aactggaaca ggcgccatat
    51481 aaaagcatca cttttataat attttgcaat ctcatcaaga gtcagtggct gaattgattc
    51541 atctattgaa gcaaagaaaa aatttgcagc ttttataaac gcaggtatca aatcctcacg
    51601 cttttcctta tacaggtttg ctatacagtc aataaccact gaacgcaaat cataatacct
    51661 atccagaact tcctgtaaaa ataaagcccg tataattgcg cgtaaaaacc atggtgtatt
    51721 tttcaaaaac agctctgcat ttagctgttc aaccccgtta atacgatata acggtgtact
    51781 ggtatcaata taaacaagag tatcattttg accaacataa ttcagggcac catcaaccga
    51841 aatcagtacc cagtttgata tctgcccatc aatacccact ttcagggaac tatcgcttat
    51901 attaaagcta taccattttt ttaattcagc cagtatctta ttaaacagga caactgcatt
    51961 tttctcatcc aaacgtttta ccagaaaatt tccaaccatc tgtggattta tttttttttg
    52021 accagcatat acggtatacc atccattgct tttaaaataa cgaaactgtt gctgtggcac
    52081 ctgtatgcca attttttttg taagtatttc cctgtattca tgataaacct ttttcagtgc
    52141 ttcaacagca tcaagagtgt cgataggggg catccgtttc catacccaat ccgattcagc
    52201 aatttttgtt ttgataaatg actgattcag cgttgatcca tgtatggtct gcatcacggt
    52261 ggatatttcg ccatatccta atatttttaa tttaagctct tcgcgataca accgtgtaaa
    52321 atctttagta aattgccggt ataaactcgt atcctgtata agattatcac gaatacggtc
    52381 atatatttca ataatagacc atatattctg cacatcaaac tgcgacagga ctaatccttc
    52441 caaatttttt gtcttatcaa attttttgaa cgttgggagg tatcgctcta aatagggcaa
    52501 ttgttcctta acaagttttt caattgaaag gttttcttca ccataaaaaa gattatacag
    52561 atagtgaagt gcacgcgtgt ttgcaatatg gcgtaccaaa tactgataaa agcgtatctt
    52621 atcaactgta agtatagctt tattaccccg catcatcgtt ataatttcat catgttgtcc
    52681 aaaaagtcct ttaataatat caatactttt tgaatgctgc acagtatctt ttaacacaaa
    52741 tcccttagat gttttattcc agatcttata aaaattttgt gacagttcct taagttcagt
    52801 atcaacagat gctaattgca taaaccgtat ccgctttcgt ttcagttcat acactttgtt
    52861 taatagttct aaatgctcta tgatatcttt atgatcatct tccataaaag cctcctcact
    52921 gtgataaggc ttgtagtgac ttctgtactg ctgcaataac taaatctgct tcccgcttag
    52981 taataataag tggaggcaaa aactgaatta ctttaggatc attccctgaa tacacaacat
    53041 atactccgtt atcaaataga agcttaacca taaaaagcgc tgtcatctca tcgttaaatg
    53101 caagacccat catgaggcca agtcctctaa ccgtaaatga agcttttgga tactgtgagg
    53161 aaagcatact tagttttttt gtaaaataat ctgccatttc attcacattg tttagcaatg
    53221 tgctatcgca catatcaaga accgccatag ctgcaaagca gccaatttca ctgcctccaa
    53281 acgttgagat atgtataaaa ggattttctt tgaaaaagaa cgcatagtct tctttatata
    53341 ctgttgcagt aattggataa atgccaccac tcatgccttt gcccagaacc acaatgtcag
    53401 gcaccacatc aaaatgttca aagccccata acttccccgt gcgaccaaag ccagtttgca
    53461 cctcatccat aataagcagt gcaccatgac tgtggcaaag cttatttact tctttaaaat
    53521 aatccttatg aggcactaca atacctaacg ttgctggaat tgtttcaagt attacagcag
    53581 cggtatgttc atcaagggca gctctcattg cttcaacatc attgaatggc acctgaataa
    53641 atccaggtga aagtggttca aatggctttt taaatttttc atcccctgtt gcaagcgcaa
    53701 gtcccgtatg cccatgataa ccaccaatag ctgaaatgat gtttggacgg cctgtaacag
    53761 ctcgtgctaa tttgatagca gtatctattg cctctccacc cgaaacacca tacacaacct
    53821 ggttcaagcc atgtggcata gtagctgcta atttttgggc cagcatagct ttaggctggc
    53881 taataaggtg atggttacca atgtcataac gctgcattgc ttttttaacc gcatccatta
    53941 tctttttatt gcggtgccct aaattaaaca caccaccgtt acaatggcag tttatgtacc
    54001 gtttcccggt aatatcatat aaaaatacac cacggcgttt atgtggcacc agcgcaatgt
    54061 catatttttt gtacatctcc accttgccgg aggacacatg acgtgcatac agttccatta
    54121 tattttcttt gttcattggc acctccattt ttatactaca acactatagt caatatgtca
    54181 aaatcatttt tttatttttg tgcgatagtt ccaacagcag tccaccgcat attaatagct
    54241 ctgttctacg ccaaatgcac ctctgccagc cagaggaata aactcatact caggatacgc
    54301 ttttttcagt gcagttgcca cctgttttgc tgtaaacagg gttgctttta caccacgccc
    54361 aatataatta gaaccagcat caactaaaaa ctgttttttg ccaccaatta ttgcgttgag
    54421 tttaatatga ccaccatgtg tgggattaaa tgcaaactct tcatgatcca gatactgata
    54481 ggtaacttca tgaggtttag ttccagcaga ataaaacttt ggtgtatgcg atgaaattcc
    54541 tgcaactgaa gctaaatatg ttatgtgctc gccaacacgt aatgaatcaa gccagaactt
    54601 aaacttttca taacacactc gttcaatatt acgccgctct ttatacctgc caatacggaa
    54661 atactgctga atgggaatga atattgattt aagattatcg tttttctggt aatctaatat
    54721 ttgctctggc acaaatgtgg tttttacaaa atgctcaatg tgtagcgata attctgaatg
    54781 tttttgatac agtggccact caaaaattat ttttaaagcc tttgaagcta atggactggt
    54841 agcaaatgtt gatgttgctt taaaaaaaat atcttcttga attggataaa ttatcccttc
    54901 ggcggcaaca aggctattat tattatgatc aatcacattt tttagaatga ttcctccatc
    54961 atactgaaga aaaacaccct gttgcctttt tttaaggtta ttattaattc ttaaaagtat
    55021 ggttccctgt gtgatagtat ctatagtatc tattttttgc cagaatgatt ccataattta
    55081 ctcaacacat tagatacata tgtatttaat agaattacaa tattttcaat acaatataat
    55141 tatgcgatag ttgcttttat taaagcttgc tttaaatcat cgaaagtaaa cggtttaaca
    55201 agataccacg aaaatccata ttgtgcataa tctgcaatag cactgttatc agcatatccg
    55261 ctcataacaa ttccttttag tttttcctga atcttcttca tctgggcaaa agcatccacc
    55321 ccacttatgc ttccaggaat agtaatatca accagtacac aatcaaatgg tttaccccgt
    55381 tcttttgctg taagtatttt atttattgca tcatcactat ggcttgccgt gtcatacgta
    55441 catcctaata tatccagcat ccctgctgta acatccaata tctcctgcct gtcgtccagt
    55501 attaatacat gtgcactgat tgaatcaaca cgcgcattat gattttcatt aacagaaaca
    55561 gtacctttaa tttttggtag atacactgta aattctgttc ccttatctga agatgatact
    55621 gtaatggtac caccatgctg tctgattatg gataacgacg tggtaagtcc taaccctgtt
    55681 cctttttctt ttgtcgtaaa ataagggtca aagatttttg atatatgttc ctggggtata
    55741 ccttttccgg tatcagaaaa cttaatcata acatattcgc cgtcaggaag ttgcaataca
    55801 tcatgtttaa gttctacttt ttttactgta actgttaaca ctccactctt gttcatagcc
    55861 tgtattgcat ttaacactat atttctaaaa acatgcgaaa tttgatgttc atcaaagtca
    55921 aaattttcta tgttgccgtc aactatgtat ccaactttaa cgctacttcc atgtgtcaca
    55981 aaatcaacag tatcatgaat taactgaatt atattacctt cttttttaat tggcttgcca
    56041 ccttttgcgt aggttaacag ctgcccagtc aatgattgag ctgatttcac agatttttta
    56101 ataccatcaa ttatttctaa tacatcattc cctttagtat tttttaacag catcaatgat
    56161 gctatttcag cattgcccat tattgcagta aggatgttat tgaaatcatg agcaatcccc
    56221 gcagccagta ttcctaatga ttcaaactgt tgcagtttta taagctcatt ttgcattgca
    56281 agcatgtcgg taatatccct gattattgta acggccccaa taatagtatt ttctatttca
    56341 atgggcgaag aagctacaga cacatctatt gttctattat tacattggca tttaagatga
    56401 taatatataa cggatttttt taacttaata cattcaccaa ttggatcaac aagtttttgc
    56461 gtagttgatt cagtaaaatt gactaaagac ctgacatctt tccccaatga tatcatatta
    56521 ttaaataata catctgcctg cttattggaa aggatgatgt tccctttatc atcagtgctt
    56581 atgaatgcat cgccaattga ttggatagta ataagcagcc gttccttttc aagaagcaat
    56641 tgctcctgag tttttttatg ccagtacaac tcatgttcaa gctgctgcgt ttttattttt
    56701 acctgcctgc gtaatgaagc attccaggca ataataccaa ttattacaag caatcctcca
    56761 aaaagcaata aagtcaggat ataccagaat tctttttcct tgtaaaatgg tcgtggtgca
    56821 agtgaaatcc atcgctggta aatagcacgt cttttacttt caggtattga attcaaagct
    56881 tttgaaataa ttgaaaaaag caccggatca tcattgcgaa cacctatgca caagttccag
    56941 tcatatcccg cattacctgc aatacgtaaa tttgtaatgc cataggtatc taccagatat
    57001 gatactgttg aaagtgagat aatcattgca tcatattcac caaaggacaa tttcatcaag
    57061 ccttcagttt cgtttttcac cgcctctata gtaaatgatg gatatttctt tttaataaag
    57121 tcaatgaccg cataaccctt cactgctgca attttcttcc cagctaaatc atccaggtta
    57181 ttaacttttg tattggattt ttgcacaaca ataacgttgg ggatgcgtac atacggcttt
    57241 gtaaactgta aatacgaatg gcgctcagga acatcctgga ttgaaagaac aatatccact
    57301 ttcctatctt tagctgcctg taacacatct gcccatgatt gtattgttgc taccgtgaac
    57361 gttactccaa gctttttact gatctcatcc aaataatcat ggcttaatcc tatatggtta
    57421 ccatcatggt cagtaaaatc aatgggtgga taatcgggac agggtgctgc aataactggt
    57481 ttttttaatg attgtatata ttttatctct tcagaagtca aatgtagaga gttagtatct
    57541 tctgatgaac agcttatgat aaatactatg gttaatatat atgctatgct attcatgcat
    57601 atatttaatt ttgctgtaca gcatgccagt aggtttttca tatcatactt tcatacaata
    57661 taacgtgaaa tatagcaaga ttaatttcac ctgtcaatgt aataatgaga tatgaatagc
    57721 ttgcgttttc aaaaatttta atgtataatt tcccaatagt ttgcaaggct ctaaaatatg
    57781 ctaccattaa ctgcttgaaa aatattcaac aatgtatccc cataattctg gaaatgtgct
    57841 tttttaagct aatgcaaaca ggtgcaaaca tataaggccg cacatcctgt tcggctgatg
    57901 ttatattttt cctatctgga aattaaccat ttctaagtat aaaaccacat tacaagcagc
    57961 ctgtgggaaa aatcgaaaac tatttttaaa agacctttat attcgttgaa gacagcaata
    58021 tttcaataat agaataaacg atgtaatact agtcattgga gttgtaagta attattatgt
    58081 atctggtagg attccacaca aaattagact tgacttcaca atggttgaca gtacccaaat
    58141 agaaaataat tttttaaaac atagcaacaa attatatgtt ttggagggta acatgaaaaa
    58201 aatatttttt atgtttttat tttttacata tttactttca ggtttaggat ttactgaaaa
    58261 taatataaaa aaaagagagg tccaggtcaa acccaatgta caaaatgttg atattgaatt
    58321 taatggtgag tatttaaagg gttatctttt tattgtagac gcaaggaccg atgaagaaaa
    58381 attaaaaaat tttaatgaaa gaaacgctaa cggatcaatt tttatttttt ttcagggaca
    58441 tgcacagaga gctgatgatg cttataaatt cacaaaggaa cttgcattaa aatcaaaatc
    58501 aggtattacc atagttccct tgtgtgatac accttatggg aagaataaag atttaagagg
    58561 cgataatggt aaggaaatta tattatttta tttaattaaa catgctctta aatctttaaa
    58621 catgaatgtt gatgaagaaa ttcaatgtaa agatattaaa attgatggca aatcttttgt
    58681 tgacttaaca aattcaataa aagttaaact tacagccatt ggatggagtc atggaggcct
    58741 gttagctcgc cgaatggcaa gcaaatatac ttcatttgaa aatcttatac aaatatgccc
    58801 tgctggattt acacaatggc cagataattc ctgtcaggca agctgttgtg tattgtcatc
    58861 ctttaactgg gaaagtttac gtattggatt tactgaaaca tttaaagggc atttctgtga
    58921 tgtaatgggt gcaggttggg gaataacgaa gggtttaacc ggtgatacag cgagatcatg
    58981 taattcatgt atttatggca atttcaatat tttaaagata tttagaccgt ataaggatat
    59041 agctgatgta acgttatatg ctgatgataa taatttccct gtggtaaata aaaaacatat
    59101 acttgtaata tttggggagg atgattctct ctttgatgat gagaaattgt tgaagtatga
    59161 taaaaatagc gaaataataa aagaacaatt ttttagtaaa tactatgaaa aatcgattat
    59221 aagtaataca aaacttgatg tcaggatttt gccagggaaa catattggcc ccataactca
    59281 ttatgatatt tacgttgata gtatagtaac ttttttggat gaaaaattaa tttaataata
    59341 attattaacc cacttgcaga aaggcaaaca aatacctgta aaacatatta aacaataaaa
    59401 gattaatttt gaataatcac aaaaagtcat agtagcagga gtaaacaaag aggtaaagga
    59461 agcagtgttt aaagaaaata gaactcatga gaggatagat cttttgataa aaatgaatat
    59521 cctgctacag aacttgaata agcaagaatg aagcatacag gacactggta ctggtggtag
    59581 aagatcagct acaataaaag gcatttacaa tacatattaa aaataatatt taacttctgc
    59641 tgcaagtgca ggcctattgt caaatgagga aaaatctgaa agattgttgc cagcaactat
    59701 cgcatataac atatagaacg tcagatcaca atcttccact atattataca ctactgacgg
    59761 tataagcata accccatgcc cctcaaaatc aagaatagat gttgcactgg cgctgaaaag
    59821 tgaagctcca gaaaaactta ccgataaccc tgcataatgc ctgttatatg tcaggaaatg
    59881 atatgcaagc gtacggtacc gctgttcatt catgccgtag ataaacgttt cataatatgc
    59941 ctgatacaat ttatcgtttt tagtcaaaca gtagccatta taaaaatact ccagcaggat
    60001 actgaaacca ctttcaaaag agtaatcata gcctgtacct gcaatatagt aagtattatc
    60061 tccatcttta tccacaagaa aacttccacg caacaagcca tcatatactg gcagggcaac
    60121 atctaaacct gcaacattcc tttgcattat cttccctgca aggattgcaa aatctatatt
    60181 gctcaaaact actttgttgc gcaaaaggta cactgaatta tcaaaactat ctccaaactc
    60241 attgttcttt cgttgaggta catagacaaa cccaacttct aaaaaatgag cagggtacag
    60301 ctcaaaacgc atggcatcca ccccctttgt ctcttctgct ccttcaaatg aaagtggtga
    60361 tattgggttt acaatatcaa gtggattcca tattttcccg cttccaaaac gtaccagttg
    60421 ccttcctaaa gtaatggttg ctttcccaat aatacatttg gcaaatgcac ggtgcagttt
    60481 taagcgatag taaccagtat cgttgcgata atcataataa tctgtatata attgatcata
    60541 ggaaaatcca atccatgttg catcaaatgt tttgctttgt gtgtaattgg atatgataaa
    60601 ttcattatca atatcagcat gaaatacaat attctggcca atatcacaat ggggtgaaag
    60661 gcgaactctg ttaacatcgg ttacaagcag cttttcttca ggaatataat ttgaagaata
    60721 atactcattg cttttataac ccataaaaag gtttttgtaa tagccgccaa tggaaaatga
    60781 aatatcccca ttctcagagc tcacctgtgc atataccatt gtcaacctgc tatgcagttg
    60841 taccattaaa cttaatactg aaagaactaa cattatgata aagcgacatt tgtatttgtt
    60901 catacatttc ccaatttgat gtaatctata caatgacagt tatattaagg aataatcctg
    60961 taaaattttt tataccacat gaagtatgta ctattttaca ttttgtaaat ctttttaaac
    61021 atataagtat atagtaaaaa tactttacct ttattcataa ataagacatt gtaattatta
    61081 tttatatttt acgcataagc tgtcaataaa agaatttaca aatcagttat tggtatatat
    61141 actgtaatgt atacacctaa ttagcggatg ctgctgtcat tacacaatat tttatttttt
    61201 caatctttat agtagcaggg atagacatgt ctttaaaaaa acaattatca cgttcaatca
    61261 ttgctttttc aaccctgata ttcatttcat ttgggatatt atttggaagc tttgtatact
    61321 acaattacca aacaagtatt tcattgctta tgaaacaaca aaacattgca ttaaaatatt
    61381 tcattgaagg ttattttata aaaatgtata attatattta tatttcaagc aacaatgatg
    61441 ccatagctaa tgtattgttg cgtgatgcca ataataaaag aactgtatta aaaatcttta
    61501 aacattttga aaatgctgat agggatataa actatcttta ttcgggatat aaagatggta
    61561 cattattaat caatgactat actccaccaa aaggttttga tccacgggta aggccatggt
    61621 atacaatagc tcttaaaaca gcaccagaaa tttcaaacgg ccttgcctat caggaaataa
    61681 aaacaaaaca atagctggta tcaataagta aagtattata tgataacaat aataccatgt
    61741 gtggcgttat agctattgat acttctttag aaaaactagc aagaataatt aataccacca
    61801 ataaaggctt ttcctcacaa cacagttttg ttataacaaa tgacggaacc atcattattc
    61861 ataaaaatcc taaaatgctg agaaaaaatt ttttcacatt gtcaaatatt acttatacag
    61921 caagtggggc atcatatagt tacaccctgg atgacataaa gaaaactgcc tatatccagg
    61981 atataaataa aattggatgg aaagtaataa cagaagtaga taattcagaa ataaccaata
    62041 tcgtaataac tcgttttttt atcagtatta tggtgattac attaattgct tctttatttg
    62101 ggctttattt aaataaaatt tttacccatg atattatcac cccaatttta tcattggcaa
    62161 aaagggttgc caacatagta gaagcaaaat cagttgatga taagtattca taccctgata
    62221 atgaaatagg agtcattgca cataatctgg agcaattaac aaaagagaaa ctgtatcaaa
    62281 aaaatatgga gcttacacaa ttaaataaat aatatactca tggatctttc atacattgat
    62341 gaattaacca gtttaaataa cagaagaaag atggaagaag aattgataaa agaatttgcc
    62401 cgctataccc ggtatggcag aatgtattcg gtaatattaa ttgatattga ttactttaaa
    62461 aagatcaatg atacccatgg acatgatgcg ggcgattttg ttttaaaaga attagcccat
    62521 atatttaaac ataattgcag aaaaacagat acaattggcc gttggggtgg tgaagaattt
    62581 ttaattctta gccccgaaac caatatatct gaagcatata atgttgctga aaatattaga
    62641 agtatcgttg agcagcattt atttaccatg atagacagag ttaccatcag tgctggcatt
    62701 ggcggatctt ttcctgatat gattgatatt tatgatatta tcaaaaaagc agatgaaaac
    62761 ctatacagag caaaacaaac aggtagaaat aaagtattat actaattata attttttttt
    62821 ggaatatagt tgcatactgc atcatatcct caaatattat tttaatcaca tcttaaaaaa
    62881 atctctatac ctatattggt aaaagcggta gtatgtaagg atgcttttta attaataata
    62941 gcattttatt attttttccc acttagaaat aaatttatag atttaatttc aatttatgac
    63001 ttattctagt ctactactac cttctatata aataatgcat tatatgaata gatggattaa
    63061 ttggtataaa ctaagtttaa ggaattccca gataaaatat tacaaaatgc agtatggtta
    63121 gtttaacggg attttttttg cttcacatcc ttcacaatgg taccatcagt tattgtaatc
    63181 aaccggtgtg cacgtttcat aaccatgggg tcatgggtgg aaaaaagaaa tgttatttgc
    63241 ttttctttat tcaggtgttc cattaaatca agaagtgcac ttcctgtttt ctgatctaca
    63301 ttggcagtgg gctcatctgc taaaactatc gccgggcttg aaacaatagc acgtgctatt
    63361 gccacacgct gctgctgccc gccagaaagt tcgtgcggaa gtcgatgcat ttcatctttt
    63421 aagcctacaa gctccagtac ttcagcagcc tttttttcac ggtaactatc gcccataccc
    63481 tgtaacagaa ggatgtactc cacattttcc agtgctgtca ataccgggat aaggttatat
    63541 gcctgaaata taaacccaat tttaaaaagg cgcaggcgcg atagttcctt tggactgagc
    63601 tttgaaatat cagtgccttc tatatataca tttccctgtg tgggataatc cagcccgcca
    63661 atgcagttga gcagtgtggt tttccccgaa cctgaaggcc cggctattgc tgcaaactca
    63721 cctttatgca atgaaagatg tacgccacgc aaggcgtgca cgatttgtcc atctgaatga
    63781 taatcctttg tgcaattgac tacctgtata atttccatac taccttccgt tgctatataa
    63841 aatgtaacgc atcaattggt tttatttttg cagccttata tgccggatat aatgatgcaa
    63901 caattgtgtt aagcagaaca atgacagttg ccacaatact attggttgct gttaaaaatg
    63961 gataaatagt agtacctgtt ccccacacgc gcattgattc actgtaaaag gcaaagttta
    64021 tacctgttgt tgcagttata gcaacaagca ggtacgtaat gacaatgcca gcaacaatac
    64081 caagcactcc taaaccgcat gcttcaagta taacaatggc agcaatccat gatggctgtg
    64141 tgccaattga tttcattatg ccaatttcac ggtatcgttc cattattgcc atgatcatgg
    64201 tattggcaat ggtgaaaata attgaaataa atattattgc taagaataca tacatcatgg
    64261 tgtcaaaaag ttttatggcc tgcacaatgt ttggtgccat ctgtttccag gtaagcactt
    64321 caaggtctgc tgtatccagt gaattaattg ttgaagcaac actatcagca aaatctttat
    64381 ccgtgcagcg gactataatt tcggatatag cattcccaag acccgagatt tcctgtgctt
    64441 tacgtatacc tgtatacacc acgtatttat caaagctttc aatgggcgat tggtatagcc
    64501 ctttgacaat aaatgcatga gcagcaagca ctccatcttt atcctgcaac attaccacac
    64561 atttatcgcc aataccaatg tcaagctttg ttgcaagtga acgtgagata agtatatcat
    64621 tgtttgaact gtccgtaata tatgaatgga tatcgctacc agcatattca aatatatttg
    64681 agacacactt ttcttcttcg ggttgaatac ccattaccag cacaccctgc gaactgcggc
    64741 ttgactgtac catagcttca aacttgacac gctgcccaat acacgccacg ccctgtatat
    64801 ttttaattgc attacacaaa tcttttggca caaatgataa tgatagcttc atatcattga
    64861 aaaaaccttt tttatgtata gccacatgtc ccagtgaagt attaatggta ttttcaagca
    64921 tctgcctgtt aaagccattc atgattgcca tggaaaacat catcaccaca agccccacca
    64981 caatggatga agcaacaatg agtgaacggc gtttattcct gaatacattg cgccaaccaa
    65041 tcattaacac tatttttaca ttttgcatcc gtatcatacg ttttttacct gattaaatgt
    65101 gccgaattgc ctcaacagga tgaagccggc ttgcacgccg tgctggaata atacaaaaca
    65161 aaagtgacac tatcatggtc agcataactg taaccacaat attggcgata gttactgtag
    65221 cagggaatat ggttgtggta ataccccaca ccgctatctc ttcagaatac tgtgagtaat
    65281 caatgggatg caccgtaaaa taataggaaa tccctgcacc caaggcaaca cccagaagca
    65341 cgccaataac tgtaatgaaa aatgtttcaa gtataaccag tgcaacaatc tgacctggcg
    65401 tggtacccac tgctttcatg atgccaagct cacgggtgcg ctcatatact gacatttgaa
    65461 ttgtgttgag aataccaaag gcaactacaa gaaacagcat cagatcaaaa agataagcgc
    65521 tgacatcatc catgattata aactgcacaa gctcaggcat tagcttttcc catcccaata
    65581 cctctacgtc attttgcaat ttttttgaaa gtgaagaggt aatagcatca atgttatcta
    65641 tatgtgaagc tttaatgaca taggcgtgaa tgtagcccat catgctgaat gtttcatcag
    65701 cctgttttag aggcatctgc agcaaaaatt tatcatattc aggatttcca cttttaaata
    65761 tgccattaat ggtaagatgt tcagctgcaa tggatccatc aaacccctgt gaaataatgg
    65821 caatagtatt gccaacgtca acctttaagt tttcggctaa tgtggcacct atcactgcat
    65881 gggtgctatc actatcgctc aggtagcggc ctttgataat gcatttatgc aagccacaca
    65941 cctgcttttc acgcacaggc tcaacaccct gtatcatgac gccttttgtt gtcccacctt
    66001 ttgcaataag ggcatcacac atgatacgtt tgcagtaagc acgtatatgt gtattactgg
    66061 atagcgcctt ttctatggat tcatctgctt caaatgcata atcaattgtt ttattttccc
    66121 agtacccttt ttcatggata tgaatatgcc ccataacaag gccagttgca tcttctatca
    66181 tctggtcgtg gccacctttg gcaaacgcat tgagcagaat aatcatggca caacccacaa
    66241 cgatagtaca cagagtaagt a
//
LOCUS       DAORYL010000006        65390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_372_length_65390_cov_12.940139, whole genome shotgun sequence.
ACCESSION   DAORYL010000006 DAORYL010000000
VERSION     DAORYL010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 65390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 65390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65390
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_372_length_65390_cov_12.940139"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PLV81_02485"
     CDS             complement(<1..815)
                     /locus_tag="PLV81_02485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOM09002.1"
                     /translation="MRYISTKEKKMFLEQRPWISEAFNSLQSIHTLPRSIYHDVETAI
                     AIARKFNKEVLEPIYLSEELKCMQDHNYLAWDFVKKAGEWRFYSLFVPKLFGGQGLNF
                     FALYPFMEEIASVCPGLGHIIFVHYLGIATLFPSMNARIINKVLRDVAKSEKEGTPRL
                     LDLTITEPEAGTDVQEPIIYGKGNINGTATKVKGGYIINARKIFISNGHLSYWHIVNV
                     WEDKKNQRETALQCVIPNGTKGFSFGRCEKKMGNLASTASELIFEDCFVPNEWV"
     gene            complement(890..2620)
                     /locus_tag="PLV81_02490"
     CDS             complement(890..2620)
                     /locus_tag="PLV81_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOM09003.1"
                     /translation="MFSLYYTIQDVKFKMRILKGFMHFLIDEAARGTILLNLPSLIKY
                     KKVARDMSWAELLEEKKYKHPNHEFLYFEDKVYTLQQMDEKINRFANYLLSIGGKPGK
                     GLAIMLENSPEFLDAFIATQKIGMYSVPINISLRGDSLVYILNHSDAEYLIIEEHLLP
                     FFEKVKDSIETIHHVIVKSDTLTNYVIPPFHTHVLKNIYSAPSKRPDIHFDDNNICFI
                     LYTSGTTGLPKGVVYRYNKSMVRLLSIIAYTFYAKDEIFYTCLPLFHANALLLTFTVA
                     LHRCGKLVLAKKFSARRFWNDIYTYNVTTFNTIGAMIPILMKQPVSEIENKHKVRKVL
                     SAACPSDEWEKFEKRFNVTLYEGYGAVDGGGNIILNVGNAPKGSIGKPGPGIEYRIKD
                     DNGNDVPINTPGELVFISKGYKKSIEYYKNEKATNDKQSGKLIHTGDLVKQDKHGYLY
                     FIGRKAEFMRIKGENVSAYEVEQIILKHPAVLEAAVYAVPSELAEDEIMAQISLVDGF
                     KLQEEDVISFLQEHLPKFAIPRYIKITKEFPKTETQRIIKKELQKAGIVEGTYDSVKK
                     QYITKAIIKH"
     gene            complement(2645..4045)
                     /locus_tag="PLV81_02495"
     CDS             complement(2645..4045)
                     /locus_tag="PLV81_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOM09004.1"
                     /translation="MRDVYVIGVHTIKFGKYIEKTIKDLTAQTVQGCLQDASLDKRHI
                     QAIWFANSAWGYAKGQECIRGQVALRPLGIDAIPITNIENACAGGSTAFHHAWLGVAS
                     GLYDITMAIGVEKLYDSNKYMVFAGFLTGLDIENINTIVANIATYGLSTEDKENLKQH
                     IAIFKNHSTPSKNKFKSIPERLKDYENLLRVYIRLTDYLGKDTVKQLRKLFSIDHSPF
                     MDVYGFAARQHMKKYGSTIEQLAHIASKNHFHSSLNPNAQYNFTISVDEVLADRIVSW
                     PLTRSMCAPIGDGAASAILCDEKTAKKLGVFTRAVKIRASVLGSGKDRLPYDMDIAER
                     LSKKAYEIAGISPTDINCAEVHDATAFGELHQTEALGFCNKGEGGIIAMRGETKIGGR
                     IPINTSGGLESRGHPVAASGLAQIHEVVTQLRHEAGQRQVPNAKLALTENGGGALGQE
                     EAAMCIHIFEAPYSKK"
     gene            4258..4875
                     /locus_tag="PLV81_02500"
     CDS             4258..4875
                     /locus_tag="PLV81_02500"
                     /inference="COORDINATES: protein motif:HMM:NF037876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOM09005.1"
                     /translation="MKKITKRQNASLRKPQIVQAFYETIIKEGFEGASLAKVAQRCGL
                     KQTLILHYFKNKENLTLACVDRAIEEYSKLLQRYIPNTDDPEKRLMDLLKALWSREYY
                     EAIHIASSFAVIDISFRNKKVKQRLERLYHIFKRFLIHELSELQKHNVIHTQDIEKTA
                     EVLMAMVEGSRHFSHFFIHEDERDKYHQSMVMAALSILKNPSGDL"
     gene            4878..6962
                     /locus_tag="PLV81_02505"
     CDS             4878..6962
                     /locus_tag="PLV81_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOM09006.1"
                     /translation="MATTFVKTICFECHCRCGVILEVVDGKITGIQGDKDHPFSHGYT
                     CPKGRACMEIVYHPDRITSPLMKVGGKESKRFEKISWEKALDVIAENLLNIREKWGAE
                     SVVFGSGTTRGMAPYLNKFLACFGSPNFMAPSNMSGGPIVLGSTATAGFSLVEPDYAQ
                     SKCMILWAHNPEASWPGLYLYDINQGLKKGAKLIVVDPRKTNFAKKADIWLQIRPGTD
                     VALLLTFLHIIIKRNLYDAEFVQQWTNGFDKLVEYVEDFTPEKCESITWIPASKIIEA
                     ATVFATTKPACIGAGMGGVCQANDAFDLVRGLTILSAITGNLEVPGGNLNCTPPTGKR
                     SCYGPDFDAARNVSKDVAKKKLGVDKYPLLMQIPCPPQVVWPAIIEGKPYHVKAIGLF
                     ANNSVCAYPNSAFVREALMKLDFLFAVDYFHTPTTELADIILPPAHWSERDDIEDLLM
                     KNHVFCQKKAVEPIPECRDEKQILIDLAEKMNLQGYWKSVEEMLNYRLEPIQMTFEEF
                     KKIGVYSTPIEYKRYEKKGTFKTPSGKVEIFAEYLTMFNLSPLPVFREPDEGPVASPD
                     LYKDYPLILTTGGRIIEYYHSSHRNIPSLRKKHPDPELQIHPETAQKLNIADGEWVYI
                     KTPRGKIEIKAKYFEGIDPRVVHAPHGFWYGVDDGWKRININMITNDNPLCPASASVP
                     IKALLCRIEKMK"
     gene            6978..8006
                     /locus_tag="PLV81_02510"
     CDS             6978..8006
                     /locus_tag="PLV81_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOM09007.1"
                     /translation="MRAVMLTGPQKMEYGDYDMPHDDGHSVIIKVDTCGICGSDIHYW
                     EIGMGMNGKRNIILGHEFCGTVYLPGSRSDLHAGDRVTALPVDPCGECYACQIGAHNI
                     CIMANKRNIIGNSVHGAYAEYVRVRPDMVRKLPDAVSNKEGAFIEPAAVALHAVNLAG
                     VTSSDTVCIIGGGPIGILCAIWAKLFDASCVVIVEVDSFRMSFIASHITVDGVIDCYD
                     TDIRKKLKTISQGGFTVAIETSATDTGLYTALAALKPRGRMVMAGINFYGQPVPTLSL
                     IAKEITVLGSMGYSIDEFDDVIENMAEKKLALEALVTHSVSLEDVPVVFNNLAQKSIE
                     AIKVVVQP"
     gene            complement(8286..10208)
                     /locus_tag="PLV81_02515"
     CDS             complement(8286..10208)
                     /locus_tag="PLV81_02515"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOM09008.1"
                     /translation="MVQCSSTSTVLITYNHQNTSPSHILLAGTSKVDITPPPGMPLAG
                     YSSNAYFSKGFRTRLYARVIYLKSDSAAIALVQCDLLAGSSLVQKKVAQLISQSTDVP
                     YTGIMMAGTHTHSGPGNYFESNFYNRFASNASGFDEDYFNFLSNQIATAIIQAYAKRK
                     PAIIAYGTKSLYGFTRNRSFIAYSANVNASADNPLKAVNPTMHMLRVDILDEGKPKPL
                     AALSVFSIHGTTVPDSNTLYSADVFAYIERELEWEMQRNGVNGFVHAVCNGTHADNAP
                     NIPKGKQGFEESKKLGVLLGQQAIGLHNELKKSGTAKAEIATILNEIDVYKNPSIGNV
                     ALCTPKVGNTLLAGASDGGPTPVVSSMPFFKEGSRSCIPCGCHKQKQVTGGPLQSLIL
                     PQEDFPHNLTFQVIKINEIFFLPIPFEVTYESGNRISKEVKQLSATPIIPIVISCANG
                     YTGYCTTPEEYSMQRYEGGHTLYGPNTQPFLALQYAQLTTTIAKTLGDSNWSKTYSMK
                     SASFLKKYPHPANPVRKAVKQPVYVVNPGIDEPCVSFLWEDVPPDQLEFNKPIVAIEY
                     SLQNDFKPFTQNEIIVDDTGYDIAVIFTGTITKEGMAIYEARWYNPPLNSNYHFRFVI
                     LPREHLNILHSEQFSW"
     gene            complement(10383..11003)
                     /locus_tag="PLV81_02520"
     CDS             complement(10383..11003)
                     /locus_tag="PLV81_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOM09009.1"
                     /translation="MVDAIHWLGHDCFKIASDVIIYTDPYNIKKQDKADIILITHEHF
                     DHCSPDDIKKIQTKDTVIVAPADCVSKLTGTVKTIKTGQTITSKGITIHAVPAYNTNK
                     QFHPKSRGWVGYVITVNKISIYLAGDTDYIPEMKSLKNIDIALLPVSGTYVMTAEEAA
                     QAALAIKPKVAIPMHYGAIVGTVADAKKFEQLLKGNIDVVIKAVEQ"
     gene            11345..>11653
                     /locus_tag="PLV81_02525"
     CDS             11345..>11653
                     /locus_tag="PLV81_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09010.1"
                     /translation="MKSSQYFLSAYNESSYIMKEKAKALFYFLMTFIILIPISMGAFN
                     IVQFQGLFGTANVILMVMLITVFIGLVLLKKGLYNASANLLVTIAALAMVMTMFYNGF
                     Q"
     assembly_gap    11654..11753
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11754..12719
                     /locus_tag="PLV81_02530"
     CDS             <11754..12719
                     /locus_tag="PLV81_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOM09011.1"
                     /translation="KESSENLKQRDVIKNLLLSVQDLTRQLTKLAEESSESSEVFSNS
                     AQSQAASAEEITSTVEEMTAGVESISDSVTRQFDMITRLIDRINELSGFIENMGKRVT
                     GASAKAEDVSKQGASVEGSLNTMNASMGSILKSSQDMNNIIEIINDIADQINLLSLNA
                     AIEAARAGDAGRGFAVVADEISKLADRTTTSIKEIAQLITINVKEIQSGFANIETMTD
                     SIRKMVGAVATISAEMKELYTSMQEQLTNNEQVRSDAQTLKNFSDSILAAIEEQKTAA
                     TEIAKSIAAVNDTAQSNAAGSIKLAENAQELLRLVRGLESEITNV"
     gene            12935..14239
                     /locus_tag="PLV81_02535"
     CDS             12935..14239
                     /locus_tag="PLV81_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM09012.1"
                     /translation="MKLKTIINVHRIFITDGWNIKTMYIKRDILSDVLEHLEKKEITL
                     IVGPRQAGKTTLMKMVQQDVLKKGGKTIFLSLDFEKDSVHFVSQQALIQKLQLECGSQ
                     KAYIFIGEIQRKENAGLFLKGLYDMELPYKFIVSGSGSLELKEKIHESLIGRKRVFEL
                     STITLQEFLQYKTDYKYHNAIKEYCRLHKEEAGYLLNEYLNFGGYPRVVLENTIKEKT
                     DILNEIYTSYIEKDISYLMKVEKIQAFKHCIRLLASQVGKCINGAELSSTLGIAIQTV
                     KNYLAYAEKTFVIIMVSPFYRNVRKELTKMPVVYFNDIGLLNFINGTSGTVDMFSGGF
                     VFQNLIFLLLKDRYKNTGASIHYWRTKDKAEVDFVINKGLEIVPVEVKYSALKNLSVS
                     RSLRNFINTYSPKEAYIVNLTLACETVINNTKVYCIPWFELL"
     gene            14264..17209
                     /locus_tag="PLV81_02540"
     CDS             14264..17209
                     /locus_tag="PLV81_02540"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOM09013.1"
                     /translation="MKAEWGIYRYPILVYRLHAIRKHIYQKISDLDAFIAISDEPIPF
                     EKNHDIRFSSIKPGAKWGKAYTCAWFHFKGKIPQQYRHSTVVCCVDIGGEGLVVNGHG
                     EPVSAINSRITFMDYLQPTWDMRVIDIDENTWDSGVIDVWVDAGFNGKIIQPFGKARF
                     KYAYLALSRDDVKDFYFDYLTLAFAYCACDDEPIKNNIKLLLDKSYAAVKNFLPHNVA
                     EAKKIISKMHDLEQKSHEVFLTAVGQGHLDLAWLWPIRESKRKAVRTLINALQNSMQY
                     PEFVFGASQPQMFEWIKQSQPVLYDAIKKAVNTGAIELQGGMWVECDTNIPCGESLVR
                     QIYYGKKFFKEEFNCDVVTCWLPDAFGFNGNIPQILKKSGIDYFCTIKLAWNEINKFP
                     YTTFNWVGIDGSSVIAHMPPEGDYTSGATPLCVKRIVENFLEKDIHNNALLVYGNGDG
                     GGGPTQTHIELIKRQQKLQWLPQVHFGKSIDYFQRLDAIQHKLPAYHGELYLEKHQGT
                     YTTQALSKYYNRRLECLLHDCEFLATYAYCNGFTYPHQFFETIWKEVLLYQFHDILPG
                     SSVKRVYDESVQRYRLLAHELQTMINTIVDYLSESGGIACINTTPFTRTEYSKGDSSW
                     YRCQMEGYSSAYMEPVDSHFSVAGGDSYIENELLRVEFAKEGHIISVFDKTHRKEICT
                     DYLNKLVLYTDKFRFFNAWDIDSNYHQKRKRELKLIDYETHTDGPQVFRRNYFRHGKT
                     TLTQDIILYAGKPYVEFSTECDYHETFTMLRADFVPSVYSDTVTCDIQFGNIARSTKN
                     STSIEKAQFEICAHKWVDVSDNDYGVSLINDCKYGHRVKDGIISLNLLRSPVYPDPYA
                     DRGRHTFRYALYPHKGRLQDSETIPLGYHFNYPPIVVKKEVNIKPLIIVDNKSIVIET
                     IKKAENEDAIVIRMYECFGNDISTTFTTGFTYKEAYEADLLERIIKKIEGNTVHFTKF
                     EIKTLLFRL"
     gene            complement(17244..18608)
                     /locus_tag="PLV81_02545"
     CDS             complement(17244..18608)
                     /locus_tag="PLV81_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HOM09014.1"
                     /translation="MPSPTQLSNKEKYSFAVAGFGQNMVITFTVTFMLVYLYEAVGFS
                     LSGIAALTAIITGAKIWDAINDFIMGMIVDHTRTRWGKLRPYILFTSFPIALLTILLF
                     AIPQTTETYKLIYFGIVYVLWGMIYTIADVPYWGLAGSITVTIPDRVSLIALARTLQA
                     IALAITTIASPLLAHWLSFAEKTTAAGWTRTAIIISVLGMGLFTLAFFNTEEKVPLPQ
                     QKQTIKQMIRAIVTNKPFLLVLIGSALNFGRSIVQVGGAVVAAIVFGDEKIFTLLGGA
                     LIAAIIISNLITPFVLRYMSKKTLMIASSIVAALVYTAMYYIGYSNLWIFVFMLFVSG
                     FFTGFFIVVQTAMIADCVDYYEYKTGERNEGVSFAGLTFISKLMGALATMAFGMVVVA
                     IGYSKGVAVTPEIQNGAFFSITILPAISCLIGTIPFIFYPLTDQKLECILKTLIERRA
                     TITP"
     gene            18897..19739
                     /locus_tag="PLV81_02550"
     CDS             18897..19739
                     /locus_tag="PLV81_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOM09015.1"
                     /translation="MKSNTIVVTGGNAGIGRAIATELVRQRYHVVIVSRNAAKGKEAC
                     DTIKKVTGNPRVDLIEGDLGSIQSVKSIAQKILKNFPDVSVLINNAGIWPQKLEINSD
                     GLEMAFMVNHMAPLILSIMLYKQLKKNKPSRIVNVNAGLYVFGNVDIEKTPYGKDFGK
                     LSTYMNTKLCNIYFTQKFSHIIENSGVTINAVHPGVIRTNLGDSSGIMGLLLRILKRA
                     WAEPEYGAQAPVWLAVSPDVENINGKFFDRFTQKPYAKNALDTSLREKLWDLSLQLAS
                     LTFK"
     gene            complement(19769..20230)
                     /locus_tag="PLV81_02555"
     CDS             complement(19769..20230)
                     /locus_tag="PLV81_02555"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HOM09016.1"
                     /translation="MCSSIKPLLLKLFFNSIPFYRRTGGRVIYISPDETEVHIKLPHK
                     WSTKNFVGTIYGGHIYSAIDGVHFTLFLRKFKNKYILWDKSSTIRFLKPARTSLIAKI
                     KIPDDEFDAIQHELKHNESVERTYTIEWKDNAGNIVAQIDKVLYFKNKKAL"
     gene            complement(20370..20861)
                     /locus_tag="PLV81_02560"
     CDS             complement(20370..20861)
                     /locus_tag="PLV81_02560"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOM09017.1"
                     /translation="MESLHPNDCLYYLVTRVSLSLTSLLKKRLVESGCDFVKPAYIGV
                     LLWLWMDDGMGTMLGKFGKDEGIKLNDLGKLAGLEPSSMTGLIDRMEKDGLVVRANDP
                     KDRRVTNIQLTQKGINVRKHIIEIMDTVTKEIFATIPKKQEDIAMDVLKKILISASNL
                     EKF"
     gene            21189..21404
                     /locus_tag="PLV81_02565"
     CDS             21189..21404
                     /locus_tag="PLV81_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09018.1"
                     /translation="MKYGDVDDIYVACELYGTERLKKVWEKTIKYDNRFIKLSVFLAR
                     AIFGLDIEGEDIKKAIHEQPVFRCFNQ"
     gene            21370..22008
                     /locus_tag="PLV81_02570"
     CDS             21370..22008
                     /locus_tag="PLV81_02570"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOM09019.1"
                     /translation="MNNQYSDALTSDAAKVLYELISTAKFLRNFVFVGGSALAMYLHH
                     RKSEDLDFFTWDKEQFNKRQILQYIDRFNEKEILNEDADILDLRLNTTKVTFFNAGWK
                     FLKPDTINNFNIASLEAIASMKIHVLFLRAKYRDYYDLYFITKKIGVKNIFNMAKNAI
                     PGLTLKLFCMALLYIDDIEDDSIAHLEPVEVISKKEIRNFLQECLLKEGLLL"
     gene            22096..22169
                     /locus_tag="PLV81_02575"
     tRNA            22096..22169
                     /locus_tag="PLV81_02575"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22130..22132,aa:Val,seq:cac)
     gene            complement(22334..22831)
                     /locus_tag="PLV81_02580"
                     /pseudo
     CDS             complement(22334..22831)
                     /locus_tag="PLV81_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
     gene            23368..25098
                     /gene="pyk"
                     /locus_tag="PLV81_02585"
     CDS             23368..25098
                     /gene="pyk"
                     /locus_tag="PLV81_02585"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOM09020.1"
                     /translation="MRKTKIVCTIGPTSESKEMIIQLVKAGMNVARLNMSHSTQELHA
                     QRIALIREVSKELNVPIGILADLQGPKIRIGTLKEKVMLEPGQQFILTTRSVPGNSVA
                     VSVSLKELPESVNKGQTILIDDGLLELRVDEVENTDIYTTVIRGGELKDNKGINLPNA
                     SIKTSSITQKDISDLEFAIKHDVDMIALSFVRSAHDVIQLRSIMEEFGGTLPIISKIE
                     KHEAVANIDDIIAASQGIMVARGDLGIEIPIEQVPIVQKMIIEKCNQQAIPVITATQM
                     LDSMIRNPIPTRAEATDVANAVFDGTDALMLSGETAFGQYPVEAVQTMARIAEYAEKT
                     VIYKATIAEKKIYTKSTITDAIALSAHESAKTLGADTILTATQSGYTARKISKYKPQI
                     PVIAVTTNHKVVNQLALSYGVIPLQIKQCNDINELVDEAIAIGRVNGLVHDGDLVVIT
                     AGILAGVTGGTNVMRIYVVAQEIARGTGFGEGVVRGIIGTSQSVELSPNTIIVADDIA
                     HIKGTKPRAVITGECSISAPIIHYCRENEIPVIGGITINEINLKQGQEVTLDIMRGTI
                     YNGTVHLPFE"
     gene            25102..26409
                     /gene="xseA"
                     /locus_tag="PLV81_02590"
     CDS             25102..26409
                     /gene="xseA"
                     /locus_tag="PLV81_02590"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOM09021.1"
                     /translation="MIEEIENTETVYSVSDITGILKQLIESTPELNGVWVKGEISNLT
                     YHSSGHIYFSLKDEGAVIQAVFFKHANKHLDFKLEEGMSVVVFGGVTVFEKRGSYQFI
                     VYKIRKEGLGELLQKIEKLKEKLHKEGLFAPERKKPLPALPRRIGVVTSPTGAAIRDI
                     IKVAMRRFPNIEIILAPAKVQGEGAVESIVTGIQELNNPQWGVDVIIAGRGGGSFEDL
                     MAFNEEAVVRAFAGSRVPIISAVGHQIDHPLSDLAADVAAPTPSAAAEIAVPVKAELI
                     AAIDNYTMRMMRRLTMMVQSFANRIMLIVRRRVFQNPMSLVENYELALADAENRIQAL
                     THKCITDTKERLSRVRDLELIIKNRLQNYWHIFMMNASKLEHLSPVSVLKRGYSITLK
                     DSQIVSSIKKIHQGDTIRVIVYDGSMQCDIQLVNEEVSFGKKG"
     gene            26393..26680
                     /locus_tag="PLV81_02595"
     CDS             26393..26680
                     /locus_tag="PLV81_02595"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOM09022.1"
                     /translation="MEKKAKKITFEDALEELEAIASKLESGELTLEQSIQYYERGVEL
                     AKFCRQKLEEAERKIEVLQKQPDGTVQKKSVKVKEDTGEIEDDEDIQGSLL"
     gene            26649..27047
                     /locus_tag="PLV81_02600"
     CDS             26649..27047
                     /locus_tag="PLV81_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09023.1"
                     /translation="MMRIYRVHFYKLLLCVAVISMVTCGKEAPLQFCEGVDKDGKPVH
                     CGKEFTTGDMTLYVMAQEPFGSNTATVIVYGKKKYSQPEYTRFDYTINPDGNTATIPL
                     SLYAEGTYIVKVIVKDKEIASGEITVIDTY"
     gene            complement(27048..27551)
                     /gene="lspA"
                     /locus_tag="PLV81_02605"
     CDS             complement(27048..27551)
                     /gene="lspA"
                     /locus_tag="PLV81_02605"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOM09024.1"
                     /translation="MKQETKNTLIAVLIFILILGSDIITKALVEKYISMYERVNVLGS
                     FVQLTLIYNQGGIFGILQGYQQFFLIISIIVFLLLVAYFILEKNKTLIFTVSMGLICA
                     GAIGNILDRITGKPGVVDFIYIGSDDVFRWPAFNIADSSIVVGATLLVIHQLIEEKKR
                     KAQNKYN"
     gene            complement(27562..30318)
                     /gene="ileS"
                     /locus_tag="PLV81_02610"
     CDS             complement(27562..30318)
                     /gene="ileS"
                     /locus_tag="PLV81_02610"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOM09025.1"
                     /translation="MDYSKTVNLPASDFPMKANLPSREPQILQKWEKEDIYKLIQKSR
                     EGNELYILHDGPPYANGHIHLGHALNKILKDIIVKHKTMSGFKAPFVPGWDCHGLPIE
                     LQVTKELGDKAKKLPKQEIRRLCRDYAQKFIDIQREEFKRLGVFGEFDNPYLTMSLDY
                     EATIVEIFGSLFEREFITKGKKPIYWCPTCVTALAEAEVEYHDHSSPSIFVKFKVDPT
                     SVTFKGVDANNLYVVVWTTTPWTLPANLAVCFHPDFDYSAYTSGNEYLIMADGLAESF
                     SAITGKTLGEKIPLPIDIIRSLKVYHPFIDRESKVIFGDFVTLDQGTGIVHIAPGHGL
                     EDYIVGLQYGLDVFCPVDDEGKFTDEFALMKGVNVFDANPKIIDLLKEKNVLIFTNDI
                     EHSYPHCWRCKQPLIFRATAQWFLLIDHNDMRQLALQVTEDVQWIPEWGKLRFKSMVE
                     TRPDWCLSRQRSWGVPIPSFYCKKCGKNQMNAETIFYFAKIARERSIESWYTDEIHSL
                     IPEGFTCECGSDDFEKEYDILDVWFDSGVSHFAVLDSRDDHRWPSDLYLEGSDQHRGW
                     FQSSLWPALALRQRAPYNTVLTHGFMLDDQGRAMSKSLGNVIPPDDIINKFGADILRL
                     WVASEDYRNDVRIGYDMIQQIADSYRKIRNTFKFMIGNCADFNPSQCVHYDELSDTDK
                     WILHKLYTLSNQVRQHYEQYEFHLVYRRILNFCTVDLSSLYFDISKDILYVEAKDSKL
                     RRANQTVLYHVTETLLRLIAPVLVFTAEEIWQFLGKESSVHADVYSELPAEWHNETLA
                     KKMDALIDIKKDVLKALEIARKEKKIGSSLEADVHIYIAGASSKELLQSMSDSNRFFQ
                     VAEVHIEDKKLDGMADYDSSSILVVKSTGKKCVRCWNYSHDIGSHPDHPELCKRCTDI
                     VLKL"
     gene            30613..30996
                     /gene="hisI"
                     /locus_tag="PLV81_02615"
     CDS             30613..30996
                     /gene="hisI"
                     /locus_tag="PLV81_02615"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOM09026.1"
                     /translation="MIELDFTKLDGLVPAVAQDYKTGEILMVAFMNKEAFELTLKTGI
                     VHYWSRSRKQLWKKGESSGNVQEVKEIRIDCDNDCVLLKINQIGDAACHTGYRSCFYR
                     VVEGGDLKVDGIKIFNPEDKYGDKK"
     gene            30993..31889
                     /gene="hisG"
                     /locus_tag="PLV81_02620"
     CDS             30993..31889
                     /gene="hisG"
                     /locus_tag="PLV81_02620"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012749648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOM09027.1"
                     /translation="MSLIKLGIPKGSLESATIELFKRAGFSISVSSRNYFPSIDDPEI
                     SCSLVRAQEMSKYVESGVLDVGLTGLDWILENESDVEIISDLIYSKVSTQKAKWVLAV
                     PNDSPIKTLEDCAGKTISTELVNFTKKYFADRNIPVKVEFSWGATEAKVVEGLVDAIV
                     EVTETGSTIRAHGLKIIYTLLETNTKLIANKQSMKDSAKRAKIKQIALLLRGALNANN
                     LVGIKMNVPEDKLADVVKLIPSLTSPTVSKLYNATWFSVESVIQESVVRDLIPKLINA
                     GADGIIEYPLNKVVSQDDVCLK"
     gene            31908..32639
                     /locus_tag="PLV81_02625"
     CDS             31908..32639
                     /locus_tag="PLV81_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09028.1"
                     /translation="MQHDVISRGRQYIKSFAVTLPAPRFYADSYWYLAIAKKISTVSS
                     VIAYCAGCMRQKGSALGHSYYHAEKVAIESAAIVLKEKGISSQSIHLAMLALIAGFLH
                     DYYREKKDHPAKAAEYVQAHLSHFMPESDIDAISFAIRNHEAFKEYQMVDNSDIMLLS
                     NALYDADKFRWGPDNFIYTIWDMIATMNIPIQDIIAHFPGGVAHINSIRYTFRSKTGM
                     DYGPEFIDQGMLIAEKLLQFLQNNS"
     gene            32790..32862
                     /locus_tag="PLV81_02630"
     tRNA            32790..32862
                     /locus_tag="PLV81_02630"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32823..32825,aa:Thr,seq:ggt)
     gene            33037..33189
                     /gene="rpmG"
                     /locus_tag="PLV81_02635"
     CDS             33037..33189
                     /gene="rpmG"
                     /locus_tag="PLV81_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOM09029.1"
                     /translation="MREVVLLSCKDCKNRNYATKKDKKKQTGKLEVMKYCKHCNKHTL
                     HKETKA"
     gene            33200..33385
                     /gene="secE"
                     /locus_tag="PLV81_02640"
     CDS             33200..33385
                     /gene="secE"
                     /locus_tag="PLV81_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOM09030.1"
                     /translation="MFNKTVQFVKESRDELKKVSWPERDEVSALTMVVVITVIITALF
                     LWLVDAALMKIVSLVMQ"
     gene            33388..33930
                     /gene="nusG"
                     /locus_tag="PLV81_02645"
     CDS             33388..33930
                     /gene="nusG"
                     /locus_tag="PLV81_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOM09031.1"
                     /translation="MTKGWYVIHTYSGHENKVKLALEKKVQNLNLGDKIFQVKIPTVE
                     EHSVKDGKKVVKPKKIFPGYVLVEMVLDDETWMAIKSIPGVTNFVGSFGTEKPRPLSE
                     KEVDSLFNKMGEVTTKDKVAVVMDFSVGEHVKVIDGPFKEFTGVIEEVYPDKGRLRVK
                     VEIFGRGTPVELDFIQVGKI"
     gene            33963..34397
                     /gene="rplK"
                     /locus_tag="PLV81_02650"
     CDS             33963..34397
                     /gene="rplK"
                     /locus_tag="PLV81_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOM09032.1"
                     /translation="MAPKKKKIVTQIKLQIPGGQANPAPPVGPALGQHGLNIMEFCKA
                     FNERTKDQQGTILPVIITVYEDRTYTFIIKTPPAAVLIKKALKLEKGSSEPNKKKVGK
                     ITQAQLEEIAKLKMPDLNAHDVEAAKKIIAGTARSMGVEVVD"
     gene            34437..35120
                     /gene="rplA"
                     /locus_tag="PLV81_02655"
     CDS             34437..35120
                     /gene="rplA"
                     /locus_tag="PLV81_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOM09033.1"
                     /translation="MKRGKRITDARAKIDKSKLYPLEEALTMMKELAFAKFDETVEVS
                     VKVIHKSYQNVRGSVVLPHGTGKDIKVLVICKGDKQKEALEAGADFVGAEDAIEKIKN
                     GWLDFQAVIATPDMMKEVGKLGPILGKRGLMPKPKSGTVTDDVKTAVKELKGGRVEYK
                     ADKTGVIHLGVGKLSFEPQKLAENIKAFYSQVVKDKPSDAKGNYIRSFHICSTMGPGI
                     KINYKGIER"
     gene            35120..35635
                     /gene="rplJ"
                     /locus_tag="PLV81_02660"
     CDS             35120..35635
                     /gene="rplJ"
                     /locus_tag="PLV81_02660"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOM09034.1"
                     /translation="MVKQYKVDFVNELKEKLASKKNVILTNYSGIKVKDMYQLRKILR
                     EKGAEYKVVKNTLFRKALKDCGYPEIDQYLVGPIGVVFVDKEVSEVAKVLKDFQKEQD
                     KFQYSVAIMDNVVYSPKDVQRIADLPSKEVLLAQVMSLINGPASGTAIAINQIMASLA
                     RGIKLVAEKNA"
     gene            35781..36164
                     /gene="rplL"
                     /locus_tag="PLV81_02665"
     CDS             35781..36164
                     /gene="rplL"
                     /locus_tag="PLV81_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOM09035.1"
                     /translation="MAKLSVQELLEAIGSLTLVEAAELVKAMEEKFGISAAAPVAVAA
                     APAAGAQAAEVEEKTEFDVILTGFGDNKINVIKEVRAITGLGLKEAKDLVEAGGKPVK
                     EKVSKEEAEKIKKQLEEAGATVEIK"
     gene            36442..39891
                     /gene="rpoB"
                     /locus_tag="PLV81_02670"
     CDS             36442..39891
                     /gene="rpoB"
                     /locus_tag="PLV81_02670"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOM09036.1"
                     /translation="MQKQQKVIRFGKIKPGIGLPNLIENQTESFKWFLQKDVAPSKRK
                     NQGLQAVFLETFPIVSPNDDIELEFVEYELGEPKYNVQECKERDVTYAAPLKATIRLI
                     KKDTMEVREQSVYMGDIPLMTESGTFIINGAERVVVNQLHRSPGIFFFWDEIERMYSA
                     RVIPDRGSWLEFEMDAKGLIVARIDRKKKFPATIILKALGYNTNEEIIKLFYDTKKIT
                     LAEEKDYEFLKNKRVASDVINPETGEVLLEAGERITIDYIDILRDEKIKVVEVIEFPK
                     NKDDAYLITSLEKDDCKSSEEALLKLHQMLRPGEPTNIDNAREIFNRLFFDPRTYSLG
                     GVGRYKINKKFGFDMKEVKEDYLRKEDIVHTVKYLINLIAEVDGYIVDDIDHLGNRRI
                     RPVGELIQNQIKIGFQRMERVIKERMTIQDVDVVTPQALISIKPITAVINEFFGSSQL
                     SQFMDQTNPLAELTHKRRLNALGPGGLTRERAGFEVRDVHPSHYGRMCPIETPEGSNI
                     GLIISMSTYARINDYGFLETPYKVVENGRVTDKVVYLTADEEDKYFIAQANAKIDENG
                     KFVDNLIPCRHRGDFPYKRPEEIQYMDVSPDQVFSVSTCLIPFLEHDDANRALMGSNM
                     QRQAVPLLTEEVPLVGTGMEGPAAMDSGVVVKAKRAGEVIYADAKVVRVKTSSGEVDE
                     YTLQKFKRTNQGTCFNQKPIVRKGQKVKVGDILADGPATDNGKLALGKNILVAFMPWM
                     GYNFEDAILLSERLVYDDVYTSLHIEQFEIEARETKLGREVITRDIPNLSEKAFRDLD
                     ENGIVRMGAYVAPGDILVGKVTPKGEQDLTPEYKLLHSIFGEKAREVRDTSLRVPNGV
                     EGIVIDVKRFSRENGDELSAGVEELVKVYIASKRKISVGDKMASRHGNKGVISRIVPV
                     YDMPYLPDGTPVDIVLNPLSVPSRMNLGQLLETELGWAAKELGFIAECPIFDSPEEQE
                     IRELLKKAGLPETSKTILYNGQTGEPFESEVMVGYIYMLKLAHMVDDKIHARSTGPYS
                     LVTQQPLGGKAQFGGQRLGEMEVWALEAYGAAYTLQELLTVKSDDMLGRARIYEAIVK
                     GINTTSPGIPESFNVLVQELRGLALDVRIYDENGNEINLSEWSEGLSKPKRKIKLETL
                     ENE"
     gene            39937..44142
                     /gene="rpoC"
                     /locus_tag="PLV81_02675"
     CDS             39937..44142
                     /gene="rpoC"
                     /locus_tag="PLV81_02675"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004753095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOM09037.1"
                     /translation="MRDFNDFNSIQIKLASPDMIREWSYGEVKKPETINYRTLKPERD
                     GLFCERIFGTTKEWECYCGKFKSIRYKGVVCDRCGVEVTHYKVRRERGGHIELACPVA
                     HIWYYRSVPSRIGLLLNLTVNELKSILYYEKYVVIDPAESDLKYGDVIDEDTFNHYYE
                     TYGDEFVADMGAAAIKEMLRRIDLDGEARKLRSKIAGQDKLKVDKKDIKRLELIEAFR
                     DSGNRPEWMILDVVPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLLLR
                     APDIIVRNEKRMLQEAVDALFDNSKRKRAVKGKANRPLKSLSDMLKGKQGRFRQNLLG
                     KRVDYSGRSVIVIGPELKLHQCGLPKKMAVELFKPFIMKRLVDKGFVQNIKSAKKMVE
                     NERPEVWEVLEEVIAEHPVLLNRAPTLHRLGIQAFEPVLVEGKAIKLHPLVCHAYNAD
                     FDGDQMAVHVPLSPRAQIECWTLMLSANNLLNPANGQPIVTPTQDIVLGIYYLTSELK
                     SESENIPYFGSMDEVLYALEFRQIKYRTKIKYLMDGEFIETTAGRLIFNEKLPAGYPF
                     VNYTVNDKELSKIISKIFREFGPTATVKVLDDIKELGYKYATYFGPTISTSDVKVPPL
                     KDELIKKSNEMLEKIENEYRNGYITNEERYNRVIDLWTKTNEDVTEVMFNTMAQDAEG
                     FNPIYIMAQSGARGSKQQIRQLAGMRGLMAKPSGEIIDVPIKSNFKEGLTVLEYFIST
                     NGARKGLADTALKTADAGYLTRRLVDIAQDVVITMDDCGTSVGIDVGALKEGDEVVES
                     IGSRVLGRTLLYDLYHPVTNEVICKSGELITEEIAEKIDELGLDRITIRSVLTCEARH
                     GVCARCYGRNLATARMVDIGEAVGIIAAQSIGQPGTQLTMRTFHIGGTASHVVEESEI
                     SFNYPVYVKETTFKTVKTEDEQGEKKTISVRRGYIVLQRLLEEIPVKSGELVVNDGDR
                     VYSGTPIVKKSDGSFIVTTKSGVVKLDKSRAYILGEPNMIPVNIGSTIYLKTDKFYHV
                     NPQTGETDVLVKFDPWFEPILTEINGKVKFIDIEINKSMREEIDPQTNQLKRIIVEAK
                     TEKLQPAIEVSSADETFNYLLPKGAHLFVDDGQTVKAGMVLAKIPKEVEKTKDITGGL
                     PRVAELFEARTPKDKATISEIDGRVKIGDTVKNKRKIIVTGDDGEEREYQIPASKYLR
                     VQDGDWINKGDKIDDGPIDPHDIIDIKGPRELEKFLVNEIQEVYRLQGVQINDKHIEI
                     IVRQMLRKVIIEDPGDTSFVVDQIVDKFEFDDENMRVEKEGGKAASAKPVLMGITKSA
                     LNTESFISAASFQETTRVLTEAAIKGKIDRLRGLKENVIIGHLIPAGTGVREYNKVTV
                     YQEVKGDLDGVAKEVEEEIPAEK"
     gene            44260..44634
                     /gene="rpsL"
                     /locus_tag="PLV81_02680"
     CDS             44260..44634
                     /gene="rpsL"
                     /locus_tag="PLV81_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012538144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOM09038.1"
                     /translation="MPTINQLIRKGRSEKVKKSKSPALKSCPQKRGVCTRVMTVTPKK
                     PNSALRKVARVRLTNGIEVTAYIPGIGHNLQEHSAVLVRGGRVKDLPGVRYHIVRGTL
                     DSLGVNDRKKGRSKYGTKRPKA"
     gene            44668..45138
                     /gene="rpsG"
                     /locus_tag="PLV81_02685"
     CDS             44668..45138
                     /gene="rpsG"
                     /locus_tag="PLV81_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOM09039.1"
                     /translation="MARRRRPAKRTILPDPKYGNIVVSKFINCMMLKGKKGVAEQAFY
                     KALELIGERTQKDPIEVFITSLENVKPLVEVKSRRVGGATYQVPVEIRPERRQALAIR
                     WLINNARSRNEKTMFQKLAGELLDAYNNTGASIKKKEDTHKMAEANKAFAHYKW"
     gene            45243..47321
                     /gene="fusA"
                     /locus_tag="PLV81_02690"
     CDS             45243..47321
                     /gene="fusA"
                     /locus_tag="PLV81_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOM09040.1"
                     /translation="MGRELPLHRTRNIGIMAHIDAGKTTLTERILYYTGKTYKIGEVH
                     DGTAEMDWMIQEKERGITITAAATTTYWKNTRINIIDTPGHVDFTVEVERSLRVLDGA
                     IAVFDAVAGVEPQSETVWRQATHYNVPRICFVNKMDRVGADFENCLTMIKERLYSIPL
                     PLQIPYGKEDSFKGVIDVVQMKALVWNDQLGEHYDVVPIPEEYHDEALYYREHLIELV
                     AEYDEEMLEKYLEGVEITETELKRAIRNATLKTALFPVFCGSALKNKGVQPLIDAITD
                     YLPSPKDIPAIRGHDVHHEDTIMERHPDDKEPFCALVFKIASDPYVGKLCYIRVYSGH
                     LAAGDTVLNVDKQKKERIGRILRMHANDREDIKEIYTGDIAALVGLRFAQTGDTITAV
                     DAPILLEKMDFPEPVIARAVEPKTRADLEKLHTVLKRLEEEDPTFFVKIDEESGQTII
                     SGMGELHLEIIVDRLQREFNVLVNVGKPQVAYKETVTAHAQAEGVFEKEIAGKLQYGH
                     VLLGISACDSSFEFVSKVDSTVVPENCIKAVEMGCRDALSSGVIAGYEMTKVRVVLED
                     AKYREDASVDHAYRIAATMAFRDAARKACPMLLEPIMKLEVVVPDEYMGDIINDINSR
                     RGKIEHIDIRGMLKVIDAYVPLAEAFGYATAVRSLSQGRASHTLQFSHYEVVPQAVME
                     KVLGSYYRPI"
     gene            47607..48821
                     /gene="tuf"
                     /locus_tag="PLV81_02695"
     CDS             47607..48821
                     /gene="tuf"
                     /locus_tag="PLV81_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOM09041.1"
                     /translation="MAKAKFDRTKTHVNVGTIGHIDHGKTTLTSAITKVLAAAYGGSN
                     KPVEFDQIDNAPEEKARGITIATSHQEYETPNRHYAHVDCPGHADYIKNMITGAAQMD
                     AAILVVAATDGPMPQTKEHVLLARQVNVPYIIVFLNKVDMLDPADRDELVELVEMEIR
                     DLLNKYEFPGDTIPIIPGSALKAMQCGCGKKECEACGPIIKLAETLDTYVPEPQRDRD
                     KPFLMPIEDVFSITGRGTVVTGRIERGIIRTGDEVEIVGFGETKKTVVTGVEMFRKIL
                     DEGIAGDNVGCLLRGTGKDEVERGQVLAKPGSITPHKKFEAEVYVLSKEEGGRHTPFF
                     SNYRPQFYFRTTDVTGIIQLPEGVEMVMPGDNIKMVVELITPVAMEETLRFAIREGGR
                     TVGAGVVAKILE"
     gene            48996..49313
                     /gene="rpsJ"
                     /locus_tag="PLV81_02700"
     CDS             48996..49313
                     /gene="rpsJ"
                     /locus_tag="PLV81_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOM09042.1"
                     /translation="MAKLTGQRIRVKLRSFDPYLLDQSAAKIVATTNRSGAKVAGPIP
                     LPTRIEKFCVLRSPHVNKKSREQFEIRTHKRLIDIIDPNSDTVEALMKLELPAGVSVD
                     IKS"
     gene            49398..50015
                     /gene="rplC"
                     /locus_tag="PLV81_02705"
     CDS             49398..50015
                     /gene="rplC"
                     /locus_tag="PLV81_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOM09043.1"
                     /translation="MKKALIGKKIGMSQMFLDDGTVVPVTVCELGPCVVVQKKTVEKD
                     GYNALKLGYGETKAHRLTKPILEDLKKKNLPLLKVFREVENFDENLQEGSVITCEIFT
                     ENQMVDVTGISKGKGFAGVIKRHGFHGGRETHGSNFHRAPGSIGANTFPAEVWKGKKL
                     PGRHGGKTVTVKNLKIVKIIKDKNLVLISGAVPGTVNSIITVQSK"
     gene            50027..50653
                     /gene="rplD"
                     /locus_tag="PLV81_02710"
     CDS             50027..50653
                     /gene="rplD"
                     /locus_tag="PLV81_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOM09044.1"
                     /translation="MVVDVYSVDGKVKGQIELNETIFNAKINDVLIYELIKAANANLR
                     QGTHDTRERSDVSGGGAKPWRQKGTGRARQGSIRAPQWKGGGVVFGPHPRDYRIELPK
                     GIRQEAYRSLLSLKFKEGAVKVVEDFSVESGKTKDMASIAKKLNVKKGVLITDSEDTM
                     LKRAIRNIPWFVYNNVKRLSSRDIFYTQTVLITESAVKIINSKYAKGE"
     gene            50656..50940
                     /gene="rplW"
                     /locus_tag="PLV81_02715"
     CDS             50656..50940
                     /gene="rplW"
                     /locus_tag="PLV81_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006235033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOM09045.1"
                     /translation="MDVNDIIIRPVITEKATELAKQNKYVFRVHKKANKDMIEKAISS
                     IFGVTPVKVNVMIVRGKRKRVRYNYGYTASWKKAIVTLKEGDKIEVFESK"
     gene            50974..51798
                     /gene="rplB"
                     /locus_tag="PLV81_02720"
     CDS             50974..51798
                     /gene="rplB"
                     /locus_tag="PLV81_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOM09046.1"
                     /translation="MGLKKYRPLTPTLRYQTVLDFSEITTDEPLKSLTKGKKSYAGRG
                     FKGQVSVRRKGGGHKRRYRIIDFKRNKYNIEGKVASLEYDPNRSANIALIVYKDGEKR
                     YIIAPDSVKVGDTIVSGENVEVKPGNAMPLKNIPDGTNIYNIELHKGKGGQLVRSAGT
                     SAVITAKEGDYCLIKLPSGEIRKIHCECYATIGEVCNKDHINVDIGKAGRSRWLGRRP
                     KVRGVAMNPVDHPLGGGEGKSSGGRHPCSPTGKPTKGYKTRKKKKYSDKMIIKRRK"
     gene            51804..52091
                     /gene="rpsS"
                     /locus_tag="PLV81_02725"
     CDS             51804..52091
                     /gene="rpsS"
                     /locus_tag="PLV81_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOM09047.1"
                     /translation="MARSLKKGPYVDIKLFKKIEEMNSTNTKKAIKTWSRRSTIFPEM
                     IGHTIMVHNGKNFIPVYVTENMVGHKLGEFAPTRTFRGHAGKDDKVAKRGK"
     gene            52092..52430
                     /gene="rplV"
                     /locus_tag="PLV81_02730"
     CDS             52092..52430
                     /gene="rplV"
                     /locus_tag="PLV81_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOM09048.1"
                     /translation="METRALSRYNRISASKARLVANEIRGFSFPEAIDILKAIPRKAS
                     KVIMKTLYSAAANAKYSNPEVRENNLYIKKVVVDEGPTMKRFRPRARGRASRIRKRTS
                     TIEIILSDEN"
     gene            52449..53129
                     /gene="rpsC"
                     /locus_tag="PLV81_02735"
     CDS             52449..53129
                     /gene="rpsC"
                     /locus_tag="PLV81_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOM09049.1"
                     /translation="MGQKVNPTGLRVGIIRTWDSVWFAEKNEYRKNLHEDIQIRKYIM
                     NKFKTAGIAKVGIERFPDRINVYIHTARPGLLIGRKGVDIENLKEELQKKVQKNVNIY
                     VQEVKKPERVAKLVAEQIAAQIENRFPYRRAIKQAITAAMRSGALGIKVNISGRLNGA
                     EMAREEAYKEGRIPLHTLRADIDYGFAEALTTFGLIGVKVWIYNGDVLSNAEEQEEEK
                     FALRKKSQ"
     gene            53150..53566
                     /gene="rplP"
                     /locus_tag="PLV81_02740"
     CDS             53150..53566
                     /gene="rplP"
                     /locus_tag="PLV81_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOM09050.1"
                     /translation="MLMPNRTRYRKVHRGRMKGKAVRGSTIHFGEYALKSIECGEINS
                     RQIEAARIAISRKVKRGGKLWIRIFPDRPFTKKPAETRMGKGKGNPEGFVARIKPGRI
                     LFELAGIDDAVAREALSLAAHKLPVKTKIISIHDNN"
     gene            53582..53791
                     /gene="rpmC"
                     /locus_tag="PLV81_02745"
     CDS             53582..53791
                     /gene="rpmC"
                     /locus_tag="PLV81_02745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOM09051.1"
                     /translation="MKGKLDELTIDELQRTLQEAKEELRKERFKRTVSRVENPKKALQ
                     LKKQIARILTLLREYELGKRTLRSK"
     gene            53810..54064
                     /gene="rpsQ"
                     /locus_tag="PLV81_02750"
     CDS             53810..54064
                     /gene="rpsQ"
                     /locus_tag="PLV81_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOM09052.1"
                     /translation="MEGKNRKQIVGKVISNKMDKTVVVEIERMMMHPKYHKFVRKTKK
                     VKSHDERNECSIGDIVLIEETRPLSKEKRYRLVKIVEKVK"
     gene            54075..54467
                     /gene="rplN"
                     /locus_tag="PLV81_02755"
     CDS             54075..54467
                     /gene="rplN"
                     /locus_tag="PLV81_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOM09053.1"
                     /translation="MIQVQTMLEVADNSGVKRVQCIKILGGTKRRYASVGDVIIVAVK
                     DAHPSYGLKDSTGKKVHGKAVLRAVVVRTTKPVRRPDGSYIRFDDNAVAIIDTKGEPR
                     GSRIFGPVARELRDRNFLKIVSLAPEVL"
     gene            54483..54797
                     /gene="rplX"
                     /locus_tag="PLV81_02760"
     CDS             54483..54797
                     /gene="rplX"
                     /locus_tag="PLV81_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020589498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOM09054.1"
                     /translation="MRTRLKKDDLVMVTTGADKGKRGKVLFVDTKRGRVIIEGVNLRK
                     KYVRPSQENPKGGQIELPYPINISNVMVFCEKCKKAVRVGIKIDGDAKYRVCKKCGKR
                     ID"
     gene            54809..55357
                     /gene="rplE"
                     /locus_tag="PLV81_02765"
     CDS             54809..55357
                     /gene="rplE"
                     /locus_tag="PLV81_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002722943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOM09055.1"
                     /translation="MAARLRILYEKEIKKRLMDKFKYSSVMQIPKIEKIVLNVGEGEA
                     HSNPNLLKSIIDELSLITGQRAVKTVARKSIAAFKIREGYDVGARVTLRGDKMYEFLD
                     RLVNVALPRVRDFRGLSPNSFDNAGNYNFSVKEQIIFPEINLDKVEKIYGINITIKTT
                     AKNASEARALLEEFRFPFQEAR"
     gene            55363..55548
                     /locus_tag="PLV81_02770"
     CDS             55363..55548
                     /locus_tag="PLV81_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002557082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOM09056.1"
                     /translation="MASERMFAKARREPKFKIRQRNRCKVCGRPRGYYRRFELCRVCL
                     RKLAGEGLIPGVKKSSW"
     gene            55573..55974
                     /gene="rpsH"
                     /locus_tag="PLV81_02775"
     CDS             55573..55974
                     /gene="rpsH"
                     /locus_tag="PLV81_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOM09057.1"
                     /translation="MSCISDPIADMLTRMRNAINAGHVKVDIPSSKMKMAIAKILKDE
                     GFIKNYKLIDDNKQKTLRIYLKYSADNQSAIIKMKRISSPGRRVYIKAEDLKPVYNNM
                     GIMILSTSKGIITNKAAKQLNIGGEALCEIL"
     gene            55988..56533
                     /gene="rplF"
                     /locus_tag="PLV81_02780"
     CDS             55988..56533
                     /gene="rplF"
                     /locus_tag="PLV81_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014910545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOM09058.1"
                     /translation="MSRIGKKPIVIPNGVSVDLSGEMITVKGPLGTLQYKLQDAINVV
                     IDNNVINIAKKDEQSKDKKVNALWGLTRALIHNMVVGVSSGFEKNLEIVGVGYRATQK
                     SNDVEFQLGYSHPVLFSPPAGIKVEVVEPTKLKVKGIDKQLVGQVAANIRKLRPPEPY
                     KGKGIRYKDEQIRRKAGKTGK"
     gene            56562..56927
                     /gene="rplR"
                     /locus_tag="PLV81_02785"
     CDS             56562..56927
                     /gene="rplR"
                     /locus_tag="PLV81_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOM09059.1"
                     /translation="MNRLKLKKVRFMRRRMRVKSKIRANTQRPRMCISRSNKNFYVQI
                     IDDSKGHTLVSASTLEKDFPITNHRSNKAAAKELGKIIAQRAVAKNIKQVVFDRNGYL
                     YHGKIKEFADAARENGLEF"
     gene            56939..57454
                     /gene="rpsE"
                     /locus_tag="PLV81_02790"
     CDS             56939..57454
                     /gene="rpsE"
                     /locus_tag="PLV81_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOM09060.1"
                     /translation="MKILKKRVKPEGLELSEKIIIINRVAKVVKGGRRFSFNAMSVVG
                     DNKGHVGIGFGKANEVPDAIRKSVEDAKKNLYKINVNKNTIPHEVIGRFKSTRVILKP
                     AAPGTGLIAGDSVRAVVEKAGIVDILSKTLGSRNPVNVTKATINGLLQLRSLKDVADL
                     REVPLPKLWQP"
     gene            57464..57652
                     /gene="rpmD"
                     /locus_tag="PLV81_02795"
     CDS             57464..57652
                     /gene="rpmD"
                     /locus_tag="PLV81_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOM09061.1"
                     /translation="MAKKLRVKQIRSYISAKPKQKATLRALGLRKINAERVHDDTPVI
                     RGMLQVVKHMVEVSEINE"
     gene            57673..58110
                     /gene="rplO"
                     /locus_tag="PLV81_02800"
     CDS             57673..58110
                     /gene="rplO"
                     /locus_tag="PLV81_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOM09062.1"
                     /translation="MQYTLKKPEYIKKKKRIGCGPGSGHGKTSCKGHKGQKARSGAHF
                     KPGFEGGQMPLQRRVPKRGFNNAQFKEEVQIINIKDIARLGIQEVTPEVLYQKGLIKS
                     QLLPVKLLANGDIASAVKVTVDAVSSSAKEKIEKAGGSVSLRS"
     gene            58137..59483
                     /gene="secY"
                     /locus_tag="PLV81_02805"
     CDS             58137..59483
                     /gene="secY"
                     /locus_tag="PLV81_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOM09063.1"
                     /translation="MPNVITNIFKIPELRKRVLFTLFILVVYRIGAHIPIPGVNIEAL
                     SSYFEMAAKSHGGGFVDFFDLFAGGALKRFTIFALGIMPYISASIIMQLLTVVIPAIE
                     KLSKEGPEGYKKINQYTRYGTVLLCALQGFGLTMWMKSMTSPKGTVVPQDAGIGFIIV
                     AVLAITTGTMILMWLGEMITERGIGNGISLIIYAGIVVRAPSAFLDTMNRIISRSDPI
                     IALILIALFIGVVAASILLTLGVRKIPVQYGKRVVGRRMVQMASQAIPLRVNAAGVIP
                     IIFASSVILFPAQISNMLGATKYAIFTKMQYWLSPGHTAYMIIYSLLIIFFCYFYTAI
                     QFNPNDLAENLKKYGGFIPGIRPGQNTADYIMHILNRITLPGSIFLALIAIVPDFIIR
                     IWPEEVSPGMAYLFGGTSLLIIVGVALDTLKQIESQLLMRHYDGFFKKGRIKGRRY"
     gene            59531..60283
                     /gene="map"
                     /locus_tag="PLV81_02810"
     CDS             59531..60283
                     /gene="map"
                     /locus_tag="PLV81_02810"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOM09064.1"
                     /translation="MAIFIYSEEEIKRVEDACRITAAVLDEIGKHVDAGITTFELDRI
                     ARDLIKKFKAIPAFLGYKGYPAAICTSINEVVVHGIPSKGQILKKGDIVGIDIGVQYN
                     GYFGDAAKTFVVGKVSNNVKKLLEVTREALERGILQCKDGNHLTDISHAIESHVVQYG
                     FQPVHEFVGHGIGRNLHEEPSIPNFGKPGKGPVIKTGMIFAIEPMINEGTYQVEILQD
                     GWTAVTKDRKFSAHFEHTVAVVNGNPKILTHN"
     gene            60312..60530
                     /gene="infA"
                     /locus_tag="PLV81_02815"
     CDS             60312..60530
                     /gene="infA"
                     /locus_tag="PLV81_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOM09065.1"
                     /translation="MAKEEPIEVEGEVVEALPNAMFRVKLDNGHVVLAHISGKMRMHY
                     IRILPGDRVTVELSPYDLSRGRITYRSK"
     gene            60554..60682
                     /gene="rpmJ"
                     /locus_tag="PLV81_02820"
     CDS             60554..60682
                     /gene="rpmJ"
                     /locus_tag="PLV81_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOM09066.1"
                     /translation="MKVRVSVKKICSDCKVIKRHGVVRVICKNPRHKQRQKKRTAK"
     gene            60701..61075
                     /gene="rpsM"
                     /locus_tag="PLV81_02825"
     CDS             60701..61075
                     /gene="rpsM"
                     /locus_tag="PLV81_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOM09067.1"
                     /translation="MARIAGVDLPNNKRVEVALTYIFGIGPSSSRKILEDAQVNPDTR
                     VKDLTDEEINQLRKIIERSYKVEGDLRTQIAMDIKRLMDIGCYRGIRHRRGLPVRGQN
                     TKNNARTRKGNRKVSVGSKKKK"
     gene            61099..61485
                     /gene="rpsK"
                     /locus_tag="PLV81_02830"
     CDS             61099..61485
                     /gene="rpsK"
                     /locus_tag="PLV81_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOM09068.1"
                     /translation="MKQKRTKKQEKKNIPVGKAFIQATYNNTIITITDMQGNVVSWAT
                     AGGEGFRGSRKSTPFAAQMAAKAAAQKAIDNANMRTIEVYVKGPGIGREAAIRSLYQA
                     GLNISKIKDITPIPHNGCRPRKRRRV"
     gene            61502..62131
                     /gene="rpsD"
                     /locus_tag="PLV81_02835"
     CDS             61502..62131
                     /gene="rpsD"
                     /locus_tag="PLV81_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOM09069.1"
                     /translation="MAKYTGPLCKLCRREMTPLMLKGRRCLTDKCAIKHKKYPPGPPR
                     KRRTKLSDYGVQMREKQKIKRSYMLLETQFKRTFDEATRLKGVTGENMLVLLERRLDN
                     VIYRLGFASSRLQARQFVQHGHVIVNGKRVDIPSYQVKVNDIVEIKEDFKTNTMLEDA
                     IKLSKAVGSVPQWLEVDYEKRSGKIIRLPERKDIEPIFNEQVVVELYSK"
     gene            62172..63137
                     /locus_tag="PLV81_02840"
     CDS             62172..63137
                     /locus_tag="PLV81_02840"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOM09070.1"
                     /translation="MSHKNLLKGFRRPSKIVFEHDEVNQSYGRFIAEPFERGYGLTIG
                     NSLRRVLLSSIEGAAITAVKIQGVPHEFTAIDGVVEDVTRIILNLKKLRFKYESELPK
                     VLHIVKDGEAELTGADFAIDSDVEIMNPETHIATIERGGRIDMEVQIERGRGYLPGEI
                     SKSSVETIGVIPIDAIFSPIRKVNIKVEDTRVGQRTDYDKLIIEIWTDGSVKPEDALA
                     QAAKIIKDHMTIFINFEEEVEEEEVAVDESHDKLKVILAKSIDELEFSVRAYNTLKSL
                     ELNTLEDVVRKTEDELKKSKHFSDQVLAEIKKKLEEHHLSLGIKE"
     gene            63152..63604
                     /gene="rplQ"
                     /locus_tag="PLV81_02845"
     CDS             63152..63604
                     /gene="rplQ"
                     /locus_tag="PLV81_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOM09071.1"
                     /translation="MKHRNSVRTLGRSHEHRRAMFANMVTSLFQHERIITTKQKGKEL
                     KRIAERLITRAKENLSLTENDSARKLHNKREVMRLIKNEDVVKKLFDDIAPRFVTRPG
                     GYTRMFLLDRREGDAAQMAIVELVVKPETKKEDKKEDKKDKKGKKTTA"
     gene            complement(63627..64478)
                     /locus_tag="PLV81_02850"
     CDS             complement(63627..64478)
                     /locus_tag="PLV81_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09072.1"
                     /translation="MHKHLIILCSTIIVLIITAPSFAVSTAVKAQNDYFLTLSLLRQI
                     RIMVENFGDETTKNKYQEIQTLFQNASEEYYGQNFDSSHQKYYKVKQELVTLTDALAT
                     LYINRAQQILDSTSKQSFDIIIKYDKNSTLKKYFQKPYNPVEEIKPYNPEEYHLFFDR
                     ETIERYLKNGYKKLQEAKNIYNNQDIIYLKSKKNIKHDELEFIIKQYLAVIDFCRLAK
                     QYGIEIHKMVKVHQLDTIQRKYGLESNKLDPIYDDRIPEEFKVDANDNISLVHSIEKA
                     RLEKKVK"
     gene            64696..>65390
                     /locus_tag="PLV81_02855"
     CDS             64696..>65390
                     /locus_tag="PLV81_02855"
                     /inference="COORDINATES: protein motif:HMM:NF021501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HOM09073.1"
                     /translation="MENEDTKKKISFRQKNPTICPVCGYEFYREEMLTGGGRLIAGKL
                     TDELRRTYEKNEKWGVIYPLAYVVTVCPQCMYAAYPKDFSTLQADEMQKINATTNARK
                     QSIDKFFGGLDFNQDRGLYHGAASYLLAMDCYSFRNKNVAPTFKMAVSAIRAAWLFGD
                     LAKLEPDKPYKKISDFFYKKAYDYYFKVVDIIQTGAEPADAAGNMGPDIDKNWGYDGI
                     LYMCAILTLKVGA"
BASE COUNT        21171 a        10832 c        14384 g        18903 t
ORIGIN      
        1 acccattcat tgggtacaaa acaatcctca aaaattagtt cactagctgt agatgctaaa
       61 ttgcccattt tcttttcgca ccgtccaaaa ctaaaacctt ttgtcccatt gggtattacg
      121 cattgcaagg ctgtttcacg ctgatttttt ttgtcttccc acacgttaac aatatgccag
      181 tatgaaaggt gtccatttga aatgaatatt ttacgagcat taataatata accacctttt
      241 actttggttg ctgtgccatt tatgttcccc ttgccataaa taataggttc ctggacatca
      301 gttcctgctt caggttcggt gatagttaaa tctagcaaac gtggagtgcc ttctttttca
      361 ctttttgcaa catctcgtaa aactttgtta ataatacgag cgttcattga gggaaataat
      421 gttgcaattc ctaagtaatg tacaaatatt atatgaccaa gaccggggca taccgaagct
      481 atctcttcca taaatgggta taacgcaaaa aagtttaatc cctgacctcc aaaaagtttt
      541 ggtacaaaga gtgaataaaa acgccattca cctgcttttt ttacaaagtc ccatgctagg
      601 taattgtgat cctgcataca ttttagttct tctgaaagat atattggttc aagtacttcc
      661 ttattaaatt ttcgtgcaat tgcaatagct gtttcaacgt catggtaaat acttcggggt
      721 aaggtatgga tggattgtaa gctattgaac gcttcggata tccatggcct ttgttccaaa
      781 aacatctttt tttctttggt tgatatatat ctcattggaa gaacctcctt attaagaaat
      841 cttcaaatat ttggtattaa atttgaatct gatagttcat aataaaacct taatgtttta
      901 taatagcttt tgtgatgtat tgtttcttta ctgaatcgta tgtaccttca acaattcctg
      961 ctttttgaag ttcttttttt attatacgtt gagtttctgt ttttgggaat tctttggtaa
     1021 tcttaatata acgaggtatt gcaaacttag gtaaatgttc ctgtaaaaaa cttataacgt
     1081 cctcttcttg aagtttaaaa ccatcaacca ggctgatctg tgccattatt tcatcttctg
     1141 caagttcaga agggacagca tatacagcag cttctaacac agcaggatgc tttagaatta
     1201 tttgttccac ttcatatgca gaaacatttt ctccttttat acgcatgaat tcagctttgc
     1261 gtcctatgaa gtaaagatat ccgtgtttgt cttgtttaac cagatctcca gtgtgaatca
     1321 atttaccgga ttgcttatca ttggttgctt tttcgttttt ataatattct attgattttt
     1381 tatagccttt agagatgaac accaactcgc caggtgtatt aatggggaca tcattgccat
     1441 tatcatcttt tattctatat tcaataccag gtccgggttt accaattgaa ccttttggtg
     1501 cgttgcccac attcaaaata atattgccac caccgtcaac agctccatat ccctcataaa
     1561 gtgtaacgtt gaaacgtttt tcaaactttt cccattcatc agaaggacat gcggcggata
     1621 gtacttttct gactttatgt ttattttcaa tttcgctaac aggttgtttc ataagaatgg
     1681 gtatcatggc accaatagta ttgaaggttg ttacattgta ggtataaata tcattccaga
     1741 atctacgtgc gctgaatttt ttagccagaa ccaatttgcc acacctgtga agtgcaacgg
     1801 taaaagttag aaggagtgca tttgcatgga atagtggaag acacgtatag aaaatttcat
     1861 ccttagcata aaaggtatat gctataatgg ataatagacg taccatagat ttattataac
     1921 ggtatactac tcctttagga agtcctgttg ttccggatgt atagagaata aaacaaatat
     1981 tattgtcatc aaagtgtata tctggtcttt ttgaaggggc actataaata tttttaagta
     2041 catgagtatg aaatgggggt ataacatagt tggtgagcgt gtcagatttt acaattacat
     2101 gatgtattgt ttcaatgcta tcttttacct tctcaaaaaa aggtagaaga tgttcttcaa
     2161 tgataagata ttctgcatca ctatgattga gaatataaac cagactatca ccccgtaaag
     2221 aaatattaat gggtactgaa tacattccta ttttttgcgt tgcaataaat gcatccagaa
     2281 attcaggtga attttctagc atgatggcca gcccttttcc tggtttacct ccaattgaga
     2341 gcagatagtt agcaaaccta ttaatttttt catccatttg ttgtaaagtg taaaccttat
     2401 cttcaaagta taaaaattca tgattgggat gtttgtattt tttttcttcc aataattctg
     2461 cccagctcat atctcttgct acttttttat atttaatgag agatggtaga tttaataata
     2521 ttgttccccg tgctgcttca tctattaaaa aatgcataaa acctttaagt attctcattt
     2581 tgaatttaac atcctgtatt gtgtaatata atgaaaacat ggtaaacctc atgtataatt
     2641 agttttattt tttactataa ggtgcttcaa agatatgaat acacattgca gcctcttctt
     2701 gccccaacgc accaccaccg ttttcggtta aggcaagttt ggcattggga acctgacgct
     2761 gacctgcttc atgccgcaac tgggtaacta cttcatgtat ttgtgccaat cctgatgcgg
     2821 caacaggatg cccgcgtgat tcaagtccgc cacttgtatt aattgggatt cttcctccaa
     2881 ttttagtttc accccgcatt gcaataatac ccccttctcc tttattgcaa aaacctaaag
     2941 cctcggtctg atgcaattcg ccaaaagcag ttgcatcatg tacttctgca caattgatat
     3001 cggtaggtga aatacctgca atttcatatg ccttttttga taagcgttca gcaatatcca
     3061 tatcataagg aagtcgatct tttcctgaac ctagtacaga agcgcggatc tttactgctc
     3121 ttgtaaaaac acctaatttt ttagctgttt tttcatcaca taaaattgcg cttgcagcac
     3181 catcaccaat tggggcacac atcgaacgcg taagtggcca gcttactatt ctgtctgcaa
     3241 gaacttcatc aacagaaata gtaaaattat attgggcatt aggatttagt gatgaatgaa
     3301 aatgattttt agatgcaata tgagcaagtt gttctattgt acttccatat tttttcatat
     3361 gttgccgagc cgcaaagcca tatacatcca taaaagggga atgatcaata ctgaaaagtt
     3421 ttcggagttg tttcacagta tctttcccta gataatcagt taaacgtatg tatactcgta
     3481 ataaattttc ataatctttc aagcgttcag ggattgattt aaatttattt tttgatgggg
     3541 tagagtggtt tttaaatatt gctatatgct gcttcaaatt ttctttatct tctgttgaca
     3601 gcccatatgt agcaatgttg gcaacaatag tattaatatt ttcaatatca agaccagtta
     3661 aaaatccagc aaaaaccata tatttatttg aatcgtatag cttttcgact ccgattgcca
     3721 ttgtaatatc atataaaccg gatgcaacac caagccatgc atggtggaaa gctgtagaac
     3781 ctcctgcaca agcattttca atattagtaa taggtatagc atctatacct aatggccgga
     3841 gagcaacttg accacgaata cattcctgtc cctttgcata accccatgct gaattagcaa
     3901 accatatagc ctgaatatgt cttttgtcca gagaagcgtc ctgtaaacaa ccctgtactg
     3961 tttgtgcggt aagatctttg atagtcttct caatatattt accaaatttt atcgtatgaa
     4021 cacctataac atatacatca cgcataaaaa gcaactccta tatgattaag aatatgatat
     4081 gagtatacat atacatgagc aggatgtctt gtatgatatt gagatttatt aaaaaaactc
     4141 agtatagtgt aatatttttt ctttgggtat tatgtgccaa tactaaccag agaaaaaaac
     4201 ttgacaaaaa tatataattt actgaatatt cagtaaatta ctaaaatgtg aatgtatatg
     4261 aagaaaataa caaaaaggca aaatgcttca ttgcggaaac cccagattgt gcaggcattt
     4321 tatgaaacaa ttattaaaga agggtttgaa ggagcgtctc tggctaaggt tgcacaacgg
     4381 tgtggtctca aacaaacact tatactccat tattttaaaa ataaggaaaa cttaacgtta
     4441 gcgtgtgttg atcgagctat cgaagaatat tcaaaattat tgcagcggta tatacccaat
     4501 actgatgatc cagaaaagcg tcttatggat cttctaaaag ctttatggag ccgtgaatat
     4561 tatgaagcaa tacatatagc ttcttctttt gctgttatag atatcagttt caggaacaaa
     4621 aaagttaagc aaagactgga acggttatat catattttca agcgttttct gatacatgag
     4681 ctatcggaac tgcagaaaca taacgtaata catacacaag acattgagaa aacagcagaa
     4741 gtactcatgg caatggttga aggctcacga cattttagcc acttttttat tcatgaagat
     4801 gagcgagata aatatcacca gtcaatggtt atggctgctc ttagcatttt aaaaaatcct
     4861 tcaggagact tatgattatg gcaactacat ttgtaaaaac tatatgtttt gaatgccatt
     4921 gtagatgtgg tgtgatactt gaagttgtgg atggtaaaat aacagggatt cagggtgata
     4981 aagatcaccc attcagccac ggatatacct gtcctaaagg tagagcatgc atggagattg
     5041 tttatcatcc tgatagaata accagtccac tgatgaaagt gggaggtaag gaaagtaagc
     5101 gttttgaaaa gatatcatgg gaaaaggctc ttgatgttat agcagaaaat ttacttaata
     5161 taagggaaaa atggggtgca gaatcagtag tctttgggtc aggcactaca cgcggaatgg
     5221 ctccatattt aaataaattt ttggcctgtt ttggttcacc taactttatg gctccttcaa
     5281 acatgagcgg aggtcccata gtactgggga gtacagccac ggcaggattt tcgcttgttg
     5341 agcctgatta tgctcaaagt aaatgtatga tactgtgggc tcataatcct gaggcatcgt
     5401 ggccaggatt atatctttac gatattaatc agggattaaa gaagggagca aaacttattg
     5461 ttgttgatcc cagaaaaacc aattttgcca agaaggcaga tatctggtta cagatcagac
     5521 ctggtactga tgtggcatta ttgctaacat ttttacatat catcattaaa cgtaatttgt
     5581 atgatgctga gtttgtacag cagtggacca atggatttga taagctggta gaatatgttg
     5641 aagattttac tcctgaaaaa tgtgaatcca taacatggat acctgcctca aaaattattg
     5701 aagcggcaac tgtctttgcc acaacaaaac ctgcatgcat tggagcaggt atgggaggtg
     5761 tgtgtcaggc aaatgatgca tttgatctgg taagaggatt aacaatatta agtgctatta
     5821 ctggtaatct tgaagtacct ggtggcaatc ttaactgtac tccaccaacg ggcaaaagaa
     5881 gctgttatgg ccctgatttt gatgctgcac gaaacgtatc aaaagatgtt gctaagaaga
     5941 aacttggagt tgataaatac ccattgctta tgcaaatccc ttgccctcct caggttgtat
     6001 ggccagcaat tatagaaggt aagccatatc atgtgaaagc aattggtcta tttgccaata
     6061 acagtgtatg tgcatatcct aattcggcat ttgttaggga agcattgatg aagcttgatt
     6121 ttctgtttgc agtggattat ttccatacgc caactactga actggctgat atcattttac
     6181 caccggctca ctggagcgaa cgagacgata tagaagatct cttgatgaaa aatcatgtat
     6241 tctgtcaaaa gaaagccgtt gagccaattc cagaatgccg tgatgaaaag cagattttaa
     6301 ttgatcttgc agaaaaaatg aatctgcagg gctactggaa atcagtagaa gaaatgctga
     6361 actataggct tgaaccaata caaatgacat ttgaggaatt taaaaaaata ggggtttatt
     6421 caacaccaat tgaatataag agatatgaaa aaaaaggaac atttaaaaca ccttcaggaa
     6481 aagttgaaat atttgcagaa tacctgacaa tgtttaatct ttcaccactg ccagtttttc
     6541 gtgaacctga tgaagggcca gttgcttcgc cagatttata taaagactat cctttaattc
     6601 ttactacagg tggccgaatt atagaatact atcattcatc acaccggaat atcccttcat
     6661 tacgtaaaaa acatcctgat cctgaattgc agatacatcc agaaacagca caaaagctaa
     6721 acattgctga tggagaatgg gtatatataa aaacgcctcg aggtaaaatt gagattaaag
     6781 caaaatattt tgaaggtatt gatccacgag tagtacatgc acctcatgga ttttggtatg
     6841 gtgtggatga tgggtggaaa aggataaata ttaatatgat caccaatgat aaccctttat
     6901 gtcctgcaag tgcttctgtt cctataaaag ctttgttatg caggattgaa aaaatgaaat
     6961 aatacaggaa tgatacaatg agagcagtaa tgttaaccgg tcctcaaaag atggagtatg
     7021 gagattatga catgcctcat gatgatggtc atagtgtgat tattaaggtt gatacatgtg
     7081 gtatttgtgg ctcggatatt cattattggg aaattggtat gggtatgaat ggtaaaagaa
     7141 acattatcct gggacatgaa ttttgtggaa ctgtatattt acctggcagt agaagtgatt
     7201 tgcatgctgg tgatagggtt actgcattac ctgttgatcc ttgcggagaa tgttatgcat
     7261 gccagattgg cgcccataac atttgtataa tggctaataa aagaaacatc attggcaata
     7321 gtgttcatgg tgcatatgct gaatatgtac gtgttagacc agatatggta cgtaagttac
     7381 ctgatgctgt aagtaataaa gaaggtgctt tcattgaacc ggcagcagtt gctctccatg
     7441 cagttaatct agcaggtgta acatcttctg atactgtttg tattattggt ggtggaccca
     7501 ttggtatttt atgtgcaata tgggctaaac tgtttgatgc atcatgtgtt gtgattgttg
     7561 aggtagatag ctttagaatg tcatttatag catcccatat tacagttgat ggtgttattg
     7621 attgttatga tactgatata cggaaaaaat tgaaaaccat ttcacaggga ggatttacgg
     7681 ttgctattga aacatcagca acggatactg gactttatac agcactagca gctcttaaac
     7741 ccagaggacg gatggttatg gctggtataa acttttatgg tcagccggtt ccaacactct
     7801 ctttaattgc aaaagaaatt acagtgttag gttcaatggg atatagtatt gatgagtttg
     7861 atgatgttat tgaaaatatg gctgagaaaa aattagcttt agaagcactt gttactcata
     7921 gtgtatcgct tgaagatgtg ccagtagttt tcaacaatct tgctcaaaaa tcaattgagg
     7981 ctataaaggt ggtcgtacag ccataataat atttttgcat aaggtgccat tcatgaatga
     8041 aatgaaaaag aagattagat ttaaaaaaag ttaggtaagc tttgtaaata aattgatgga
     8101 aaatattgtt gcaactactc attactttaa gtgcccattt aaagataaaa aagagcagat
     8161 attgtaatgg ttgtgtatta aggaaaatta atgatatgaa agccccagca tatgaccagt
     8221 aagcggatta caataatcaa ataactggat aagcatagtc atattgaaaa taaggctttt
     8281 atatactacc aggaaaattg ttctgaatgt agtatattta gatgttctcg tggcagaatt
     8341 acaaatctaa agtggtaatt tgaattaagt ggaggattat accagcgtgc ttcatagatt
     8401 gccatccctt cttttgttat tgtgcctgta aatatcactg ctatgtcata gccagtatca
     8461 tccactataa tttcattttg agtgaatggc ttaaaatcat tctgcaaact atattcaata
     8521 gcaacaattg gtttgttgaa ttctaattga tcaggaggta catcttccca caaaaaagaa
     8581 acgcagggtt catctatccc tggatttaca acataaacag gttgttttac tgctttacgt
     8641 acaggattgg cagggtgtgg atattttttt aagaatgagg cacttttcat actgtaggtt
     8701 tttgaccagt tactatcgcc caatgttttg gcgatagttg ttgttaactg agcatactgc
     8761 aatgcaagaa aaggttgagt atttggccca tacagggtgt gtccgccttc gtaacgctgc
     8821 atggaatact cttcaggtgt ggtgcaatac ccagtgtatc cattggcaca acttatgacg
     8881 attgggataa taggtgtggc agataattgc tttacctcct tactgatacg gttgcctgat
     8941 tcatatgtta cttcaaatgg tatgggaaga aaaaatattt catttatctt gattacctga
     9001 aatgtgagat tgtgagggaa gtcttcctgt ggaaggatta atgattgcaa tggccctcca
     9061 gtgacctgtt tttgtttatg acatccgcag gggatacaac ttcgtgaacc ttctttaaag
     9121 aaaggcatac tgcttacaac tggtgttggg cctccatcgc ttgcacctgc cagcagtgtg
     9181 tttccaacct ttggtgtgca caatgctaca ttgcctattg agggattttt atatacatca
     9241 atttcattga gaattgttgc aatttcagcc tttgctgttc cacttttttt taattcattg
     9301 tgcaatccta tagcctgttg tccaagaaga actccaagct tttttgattc ttcaaaaccc
     9361 tgtttccctt ttggaatgtt aggtgcgttg tcagcgtgag taccattaca tacagcatgc
     9421 acaaatccgt tgacaccatt tcgttgcatt tcccattcta attcacgttc aatataagca
     9481 aacacatcgg cactgtataa tgtgttagaa tcgggtactg ttgtaccatg aatggaaaaa
     9541 accgataaag ctgccagtgg ttttggtttg ccttcgtcta gtatatctac cctgagcatg
     9601 tgcatcgtag gatttaccgc tttcaaaggg ttatcagcac tggcgttgac atttgcgctg
     9661 tatgcaatga aactacggtt acgggtaaat ccatataatg attttgtgcc gtatgcaatt
     9721 atggcgggtt tgcgtttggc gtatgcctga attattgcag tggctatctg gttggataaa
     9781 aaattgaaat agtcttcatc aaatccagac gcattagaag caaaccgatt ataaaaattg
     9841 ctttcaaagt aattcccggg gcctgaatga gtgtgagttc ctgccatcat aatgcccgta
     9901 taggggacat cagtggattg tgaaatgagc tgagcaactt ttttctgtac caggcttgaa
     9961 ccagccagca ggtcacattg gactaacgct atagcagcag agtctgattt taaataaata
    10021 acacgggcat aaagccgtgt cctgaatccc tttgaaaaat atgcattgga agaataacct
    10081 gccagtggca ttcctggtgg gggagtaata tcaacttttg aagttcctgc aagcagtata
    10141 tgtgaagggg atgtgttctg gtgattatat gttatgagca cagtactggt tgaactgcat
    10201 tgtaccaaaa atagacacca tataatgtac gttattatat gtttaagaga acgcataaat
    10261 atactatctc ctgcattata aaatgagcgt tcgctcacta tatgcgaata acactcatat
    10321 gtcaaatatt ttttattgct aaaatctaga ataacagaaa ttggtcagga aactctactg
    10381 ctttactgtt caaccgcttt aatcacaacg tcaatgtttc cttttaaaag ctgctcaaat
    10441 tttttagcat cagcaacagt tcccacaatt gctccatagt gcatggggat tgctactttt
    10501 ggcttgattg ccagtgcagc ctgcgcagct tcttcagcag tcatgacata tgtaccggat
    10561 accggtagca gtgcaatatc aatattttta agggatttca tttcaggtat gtaatcggta
    10621 tcgccagcta agtaaatgct gatcttattc actgtgataa cgtatcctac ccatcctctg
    10681 gatttgggat gaaattgttt gttggtgttg taagcaggaa cagcatgtat ggtaatccct
    10741 ttactagtta ttgtttggcc tgtttttatt gtttttactg ttccagttag tttgcttacg
    10801 caatcagcag gtgcaacaat gacagtatct ttggtttgaa tcttttttat atcatcaggt
    10861 gaacagtggt caaagtgttc atgggtaatc aggattatat ctgctttatc ctgcttcttt
    10921 atattgtatg ggtcggtgta gattattacg tcactggcaa ttttaaagca gtcatgacct
    10981 aaccagtgaa ttgcatccac catagttgtt acctccttaa ggttttttgc actatcattt
    11041 ttagcaaagc acaaacttac acagcataag gttattgttg caataatcgt gatttttttc
    11101 ataagaacct cctccttatt taaagagcaa gactaaatgt tgtgttaaaa caatttcatc
    11161 gcacagatgc tgaacacgac catgcttaag tgtttacaat taaagctata agcttttgtt
    11221 taaaaatgta atagtataaa accaaaatat caatctaaaa atattataat gataccaaaa
    11281 aagtattgct attttttatt taatacttca ttgtaatcat tgtatagttt ttgggaggaa
    11341 tattatgaaa tcgtctcagt attttctatc cgcatataat gaaagttcat acattatgaa
    11401 agaaaaagca aaagcgttgt tctattttct catgaccttc ataatattaa tcccaatatc
    11461 catgggagca tttaatattg tccagtttca ggggttattt ggaactgcaa atgtgatatt
    11521 aatggtgatg ctgataacag tttttattgg gttggtgtta ttaaaaaaag gcctctataa
    11581 tgccagtgca aacctcctgg taacaattgc agcgttggct atggttatga ccatgtttta
    11641 taatggtttt cagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaaagaat
    11761 catcggaaaa cctgaagcag cgagatgtga taaagaattt attactatcg gtacaggatt
    11821 taacacgaca gctaacaaaa ttagcagaag aaagttcaga gtcatcagag gtattttcaa
    11881 acagtgctca gtcgcaggcg gcttcagcag aagaaataac atcaacagtg gaagagatga
    11941 cagcaggcgt tgaaagtata agtgattctg ttaccagaca gtttgatatg attacccgtc
    12001 ttattgatcg cattaatgaa ttatcgggtt ttattgaaaa catggggaag cgtgtaaccg
    12061 gagcatcagc aaaagctgaa gatgtttcaa agcagggggc atcggttgaa gggtcactca
    12121 ataccatgaa tgccagcatg ggtagcatct taaaaagctc tcaggatatg aacaatatca
    12181 ttgaaatcat aaatgatata gctgaccaga taaatttgct ttcactgaat gctgctattg
    12241 aagcagctcg tgcaggtgat gctggccgtg ggtttgctgt ggttgccgac gaaatatcaa
    12301 aattagccga caggacaacc accagtatca aggagatagc tcagcttatt accataaatg
    12361 tgaaggaaat acagagtggg tttgccaata tagaaacaat gactgatagc atacgaaaga
    12421 tggttggtgc agtagcaacg atttcagcag agatgaaaga attatacact tcaatgcagg
    12481 agcagctaac taataatgag caggtacgca gtgatgcaca gacattaaag aatttttcag
    12541 acagcattct tgcagccata gaagagcaga agactgcagc aacagaaata gctaaatcaa
    12601 tagctgcagt caacgatact gcacagagca atgcagccgg atcaataaaa ttagcagaaa
    12661 atgcgcagga actactacga ttggtgcgag gattggaatc tgaaattacc aatgtctgat
    12721 gtgttgtaag tgtgtatttg ttaatcgtga ttattattga ttgatggtgc aatgtttata
    12781 aaaagtaaaa aatatgaaca cataaattat ttttataata cgatataaat aatacttgac
    12841 tttaataatt atcgtgttaa tttataagta tatcgattct tataataata ctattggtat
    12901 actccatgta cagagtaatg taagcttctg aaaaatgaag ctgaagacaa ttataaatgt
    12961 gcatcgtata ttcattactg atggctggaa tattaaaact atgtatataa aaagggacat
    13021 attgtctgat gtacttgaac atttagaaaa aaaagagata accttaattg ttggtccacg
    13081 tcaggcgggt aaaaccacgc tcatgaaaat ggttcagcaa gatgtattaa aaaaaggagg
    13141 aaaaacaata tttttaagtt tagattttga aaaagatagt gttcactttg tatcacaaca
    13201 ggctttgatt caaaaattac aattagaatg tggatctcaa aaagcataca tatttatagg
    13261 tgagatacag cggaaagaaa atgcagggct gtttttaaaa ggcctctatg acatggagct
    13321 tccatacaag ttcattgttt caggttcagg tagccttgag ttaaaggaaa agattcatga
    13381 atcgcttatt gggcgtaaaa gagtatttga attatctacc ataacacttc aagaattttt
    13441 acaatacaaa acggattata aatatcacaa cgccattaaa gaatattgta gattgcataa
    13501 agaagaagca ggttatttac tcaatgagta tttgaatttt ggaggatatc cccgtgttgt
    13561 gttagaaaat acaataaaag aaaaaacaga tatactgaat gaaatatata caagttatat
    13621 tgaaaaggat atatcatatt taatgaaagt tgaaaagatt caggcattta aacactgcat
    13681 acgtttgctg gcttctcagg tgggcaaatg tataaatggt gctgaattat catcaacctt
    13741 aggtattgct attcaaacag taaaaaatta tttagcgtat gctgaaaaaa catttgtcat
    13801 tatcatggtt tccccctttt acaggaatgt tcgcaaggag ttaactaaaa tgcccgtggt
    13861 ctattttaat gacattggat tattaaattt tattaatggt acatctggta cagtggatat
    13921 gttctcaggt ggttttgttt tccagaatct tatatttttg ttattgaaag atagatataa
    13981 aaacacaggc gcatcaattc actactggag gactaaggac aaagctgaag tggattttgt
    14041 gatcaataaa ggattggaga tagttcctgt tgaagttaaa tacagtgcac taaaaaatct
    14101 cagcgttagc aggtctttgc gaaattttat caacacatat tcaccaaaag aggcatatat
    14161 tgttaactta acattagctt gtgaaacagt aataaataac acaaaagttt actgtattcc
    14221 ctggtttgag ttattgtaga tatagcttta ccatgagaag agtatgaaag ctgaatgggg
    14281 catatataga tatccaattc tggtatacag gttacatgct atccgaaagc atatctacca
    14341 gaagatttca gatcttgatg cattcattgc tatcagtgat gagccaatac cctttgaaaa
    14401 gaaccatgac atacgcttta gcagcataaa acctggcgca aagtggggta aagcatatac
    14461 ctgtgcctgg tttcatttca aaggtaaaat cccccagcag tacagacata gtactgttgt
    14521 gtgctgtgtt gatatcggcg gagaagggct ggtagtcaat ggacatggtg agccagtaag
    14581 tgcaatcaac agccgtatca catttatgga ttacctgcag ccaacctggg atatgcgtgt
    14641 tattgacatt gacgaaaaca catgggattc aggtgtaatt gatgtatggg ttgatgcagg
    14701 atttaatggg aagataattc agccatttgg gaaagcccgt ttcaagtatg cataccttgc
    14761 actgagccgt gatgatgtta aagattttta ttttgactac ctgacgcttg catttgccta
    14821 ttgtgcctgc gatgatgagc ctataaaaaa taacatcaag ctgttgctgg ataaatccta
    14881 tgcagcggta aaaaattttt taccacacaa cgttgcagaa gctaagaaaa ttatttcaaa
    14941 gatgcatgac cttgaacaaa agtcccatga agtattcctt actgcagtgg gtcagggaca
    15001 tctggattta gcgtggctat ggccaataag ggaatcaaag cgcaaagctg tgcgtacact
    15061 aatcaacgca cttcagaata gcatgcaata tcctgaattt gtgtttggtg caagccagcc
    15121 gcagatgttt gagtggataa agcaaagtca gcctgtactc tatgatgcaa taaaaaaagc
    15181 agtaaacact ggtgctattg aattgcaggg tggcatgtgg gttgagtgcg ataccaatat
    15241 accctgcggt gaatcgctgg tgcgccagat ttactatggg aaaaaatttt ttaaagagga
    15301 attcaactgt gatgtagtaa cctgctggct tcctgatgca tttggtttta acggtaacat
    15361 tccgcagata ttaaaaaaat caggtattga ctatttctgt acaatcaagc ttgcgtggaa
    15421 tgagatcaat aaatttcctt acacaacatt taattgggtt ggcattgatg gcagcagtgt
    15481 catagcccac atgccacctg aaggtgatta cacaagtgga gcaacaccgc tgtgtgtaaa
    15541 acgcattgta gaaaattttt tagaaaagga tatccataac aatgcccttc ttgtctatgg
    15601 gaatggcgat ggtggtggtg gtcctacaca aacacatatt gaactgatca agcggcagca
    15661 aaaactgcaa tggttaccac aggtgcattt tggcaaatca atagattatt ttcaacggct
    15721 ggatgctatt caacataagc ttcctgcata tcatggtgaa ctgtacttag aaaagcatca
    15781 gggaacctac accacacagg ctttaagtaa atattacaac cgtagattgg agtgcctgct
    15841 tcacgattgt gaatttctgg caacgtatgc atattgtaac ggctttacat atcctcatca
    15901 attttttgaa actatctgga aggaggtttt gctgtatcag tttcatgaca tcttgcctgg
    15961 ttcaagcgtc aaaagggttt atgatgaaag tgtgcaacgc tacaggctgc tggcacatga
    16021 actgcaaaca atgatcaaca ccatagttga ttatctttca gagtctggtg gcatagcatg
    16081 tattaataca acacctttta cccgtacgga atatagtaag ggcgatagtt catggtatcg
    16141 gtgccagatg gaaggatatt ccagcgcata catggaacct gtcgattccc atttttcggt
    16201 tgcaggtggc gacagttaca ttgaaaatga actgttgcgg gttgaatttg caaaagaagg
    16261 ccatatcatt tctgtatttg ataaaacaca tagaaaagag atttgcacag attatctgaa
    16321 taagctggtg ctctacactg acaaattcag gtttttcaat gcgtgggaca ttgacagtaa
    16381 ctatcaccaa aaaagaaaaa gagaactaaa acttatagat tatgaaacac atactgatgg
    16441 tccacaggtg ttcaggcgaa attactttcg tcatggcaaa acaaccctta cacaggatat
    16501 tattctgtat gctggaaagc cttatgttga attttccact gagtgtgatt accatgaaac
    16561 gtttacaatg ctcagagctg attttgtacc atccgtgtac agcgatacag ttacctgcga
    16621 tattcaattt ggcaatattg cacgaagcac caaaaacagt acatcaattg aaaaagcaca
    16681 gtttgaaatt tgtgcgcaca aatgggtgga tgtttcagat aatgattatg gtgtatcgtt
    16741 aatcaacgat tgcaaatatg gacacagggt taaggatggt attatcagcc tgaatctatt
    16801 gcgttcgcca gtgtatcctg acccttatgc agatagaggc agacacacat tcagatatgc
    16861 cctgtatccg cataaaggca gactgcagga tagtgaaacc ataccgcttg ggtatcactt
    16921 taattatccg ccaattgtag taaaaaaaga ggtgaacata aaaccattaa ttattgtgga
    16981 caataagagt attgttattg aaactatcaa aaaagctgaa aatgaagatg ctattgtaat
    17041 caggatgtat gaatgttttg gcaatgatat atccacaaca tttactactg gctttacata
    17101 taaagaggcg tatgaagccg atttgctgga acgtattatc aaaaagatag aagggaatac
    17161 tgtgcatttc acgaaatttg aaattaaaac ccttttgttc aggttatagt aaataaatta
    17221 ttttcacttg tgatgtatta atgttatggt gttattgtag ctcttcgctc aattagtgtt
    17281 ttgagtatgc attcaagttt ttgatcagtc aaaggataaa aaataaatgg tatggtgcca
    17341 atgaggcaac tgattgccgg caatatagtt attgaaaaga aagctccatt ttgaatttca
    17401 ggtgtaactg ccaccccttt tgaatagcca atagctacta caaccatgcc aaatgccatt
    17461 gttgcaagcg cacccataag ctttgaaata aatgtaagcc ctgcaaaact tacgccttca
    17521 ttgcgctcac cagttttata ttcataataa tcaacgcagt cagctatcat ggctgtctgc
    17581 accacaataa aaaacccggt aaaaaaacct gagacaaaca gcatgaaaac aaaaatccac
    17641 aggttgctgt agccaatata gtacattgct gtatacacta aagctgccac aatactggaa
    17701 gctatcatca atgtcttctt tgacatatac cgtaaaacaa atggggtaat caggtttgat
    17761 ataattattg ctgctataag tgcaccgcca agcagagtga atattttttc atcaccaaat
    17821 actatcgccg ccaccacagc tccaccaacc tggacaatac tacgtccaaa atttaatgca
    17881 ctgccaatca atactaaaag gaatggttta ttggtaacta tcgctctaat catttgcttt
    17941 atggtttgct tttgctgtgg aaggggtacc ttttcctctg tgttaaaaaa tgctaatgta
    18001 aaaagtccca taccaagtac tgatataatg atagcagtgc gtgtccagcc tgcagcagtg
    18061 gttttttcag caaagcttaa ccagtgggca agcagggggc ttgcaatggt ggtgattgct
    18121 aatgctattg cctgtaatgt tcgtgcaagg gcaatgaggc ttaccctatc aggtatggta
    18181 accgtaatgg aacctgccag tccccagtag ggaacgtcag caatggtgta tatcattccc
    18241 cacaatacat agacaatgcc aaaatagatt agtttatatg tttcagttgt ttgtggaatt
    18301 gcaaaaagca gaattgttaa aagcgcaatg ggaaaagaag tgaataaaat atacggacgt
    18361 aatttgcccc accgggtgcg ggtatggtca acaatcatgc ccataataaa atcgttaata
    18421 gcatcccata tcttggcacc ggtaataatt gcagtgagtg cagcaatacc tgacaggctg
    18481 aatccaacag cttcatacag gtataccagc atgaaggtta ctgtaaacgt gataaccata
    18541 ttttgcccaa atccggcaac agcaaaagag tatttttcct tgtttgatag ttgtgtgggt
    18601 gaaggcatga ttttctccta taaagaaaga ataattcagg tcaaaaatta ccagtacctg
    18661 tgttgtacac tgtcatgctg aacatgatcc aggatctatg tatcaatata attatcgcat
    18721 cagtcaacat ttaaatcaat acacataatg aagaataaaa actattccat agtttatggg
    18781 ttactttaca tttagcaata aagagtaatt tatccgcaat aattgaaata ataatacaat
    18841 tgacaatcag gttttttatt actatgatta aagaaaatca ttaaaaagga tatactatga
    18901 aatcaaacac aatagtggta accggtggta atgcaggaat aggcagagct attgcaacgg
    18961 aattagtcag gcaaaggtat cacgttgtga ttgtttcgcg caatgccgct aagggtaaag
    19021 aagcatgtga cacaataaaa aaagttacag ggaatccgcg cgttgacctg attgaaggtg
    19081 atcttggttc catacaatct gttaaaagta ttgcacagaa gatactgaaa aattttccgg
    19141 atgtttcggt gttaatcaat aacgcgggaa tatggccaca gaagcttgag attaattctg
    19201 atgggctgga gatggctttt atggtaaatc acatggcacc gcttatcctg agtatcatgc
    19261 tgtataagca actgaaaaaa aataagccat cacgaatagt taatgtaaat gcagggttgt
    19321 atgtttttgg caatgttgat atcgaaaaaa ccccttatgg aaaagatttt ggaaaacttt
    19381 caacctatat gaatacaaag ttatgcaata tttattttac gcagaagttt tcacacatta
    19441 ttgaaaacag tggcgtaaca ataaatgcag tgcatcccgg cgttatacgg acaaatttag
    19501 gcgatagttc cggaataatg ggattattgt tacggatttt aaaacgagca tgggcagaac
    19561 ctgagtatgg tgcacaggcg ccagtatggc ttgctgtaag ccctgacgtt gaaaatatca
    19621 atggaaaatt ttttgacagg tttactcaga aaccgtatgc aaaaaacgcc ctggacactt
    19681 cattgagaga gaagctgtgg gatttaagct tacagctagc atcactgacg tttaaataaa
    19741 gtatatgtaa caatcttgat ttggcggttt ataatgcttt tttattctta aagtatagta
    19801 ctttatcaat ttgggcaact atatttccag cgttatcttt ccactcaatg gtatatgttc
    19861 tttcaactga ttcattgtgt tttagttcat gctgtatagc atcaaactcg tcatctggaa
    19921 ttttaatttt agcaatgagt gatgttcgtg ctggcttaag aaaacgaatg gttgatgatt
    19981 tatcccacaa tatgtattta tttttaaatt tccttaaaaa taaggtaaag tgcacaccat
    20041 caattgcgct gtagatatgt cctccataaa tggtgcccac gaaatttttt gtgctccatt
    20101 tatgtggcag ctttatatgt acttctgttt cgtcaggaga tatgtagata actctcccac
    20161 cggtccttcg gtaaaagggg atggaattaa aaaataattt caggagtaaa ggttttattg
    20221 aagagcacat gttgtaacct ccatgatgat tatgtataca tacaatatag ctgtcatgta
    20281 tataattgtg ccacgttatg aggcatgcaa agtagatata ggcaaaaaag tggtatttgc
    20341 atataatatt ttattgcctc cctgtgattt taaaatttct ccaggttact ggcgcttatt
    20401 aaaatctttt ttaacacatc cattgcaatg tcttcctgtt tcttggggat agtggcaaaa
    20461 atttcttttg taacagtgtc cattatttca atgatgtgct ttctgacatt aatgcctttt
    20521 tgggtaagtt gaatgttggt gaccctgcgg tctttggggt cattagctcg aactaccagg
    20581 ccatcctttt ccatgcggtc aataaggcca gtcatgctgg aaggttccag tcctgcaagc
    20641 tttccaaggt cattgagttt aataccttca tcttttccaa attttccaag catggttccc
    20701 atgccatcat ccatccatag ccataaaaga acaccaatat atgccggttt aacaaaatca
    20761 catcctgatt ccaccaaacg tttttttaac agtgatgtta gcgatagcga tacacgggtt
    20821 acaaggtagt acaggcaatc gtttggatgc agtgattcca tagtaatcct tttgtagcaa
    20881 attattacgt atacatataa tatgtatacg taatattatc aaggatattt tcaatataga
    20941 tatatcacta acaacagtac ctacataatg ttaatatttg tgcttgataa tctacggttg
    21001 tatattacaa tagtgttatg taaaaaaact tattggtaat ttgagaaaat ttttttagaa
    21061 attgcaaaac catggactta gccaaaaaaa aacaaaaact ttacaatagc cttaaaaaac
    21121 gtggattatt gtggtcgtat aatactaatc atataaattt ccctgatgat attctattgg
    21181 agcatataat gaaatatggt gatgttgatg atatatatgt tgcctgtgaa ttatatggta
    21241 cagaacgatt aaaaaaagta tgggaaaaaa caataaaata tgataaccgt tttataaaac
    21301 tcagcgtttt tttagcacgt gctatctttg gattggatat tgaaggagaa gatattaaaa
    21361 aggcaatcca tgaacaacca gtattcagat gctttaacca gtgatgcagc aaaagtactt
    21421 tatgaactta tatctacagc aaaattttta cgtaattttg tttttgtagg tggttctgct
    21481 ttggctatgt atctgcatca ccggaaaagt gaagatttag atttttttac atgggataaa
    21541 gaacaattta acaaacgtca gattttacag tatatagacc gttttaatga aaaagaaatc
    21601 ttaaatgaag atgctgacat tcttgatttg cgtttaaaca ctacaaaagt gacatttttt
    21661 aatgcaggtt ggaaattttt aaaaccggat actatcaata attttaatat tgctagcctg
    21721 gaagcaatag cttctatgaa aatacatgta ctttttttgc gtgcaaaata cagagattac
    21781 tatgaccttt attttataac aaaaaaaatt ggtgtaaaaa atatttttaa tatggcaaaa
    21841 aatgcaattc cgggactcac attaaaactt ttttgcatgg cgttgctcta tattgatgat
    21901 atagaggatg attctatagc acatcttgaa cctgttgaag taatttcaaa aaaagagata
    21961 cgtaattttt tacaggaatg tttactaaaa gaggggttgc ttttataagg cttcaatcag
    22021 cacatccact tccaaatata cttgacaaaa caattttacg atgttgtatt gattacacat
    22081 agcagaatag caaaagggcg attagctcag caggttagag cgcctgcctc acatgcagga
    22141 ggtcactggt tcgaatccgg tatcgcccac atccgcaaag aaatccagct agctcatttc
    22201 catccatggt gtactgacga atgaggattt tttcacagta actatcgcca attgcaatgc
    22261 cacatatata tttcttccat acaaaaaagt ttgctaaaag taactcattg gggtttgtta
    22321 ctgtatcata ctcaagagca tgcgtaagcg atagttcctg tgatttggca attgcctctt
    22381 taagggtcca caggtgtaaa aagcgtttgg ttttgcccca tggcaggtga ttaatatatt
    22441 gatgctcaga tggagcaaaa tagcgttgta tgattgcgtt tcgatatgcg acgtgtcggg
    22501 tatatggttc aatgtcaatg ccatgcggcg tataagacaa agataccgca acagcgctgt
    22561 gtgtgtgtga cagtgatata tacaatggct gtttgttgtg taatgttttt ttcgtagcga
    22621 agttgtaatg tagtgtctca aagcctgtaa gctttgccag cgctttcaat gcacacctgg
    22681 atactaaaaa ttcagccttc ttgctatcgg attgtaatgt attgtactgg tgcttttcgg
    22741 tataagaaag taatgaaaaa agatattctc tgtcttgtgg tgatatggtg gtgtagtgaa
    22801 tacacaggaa atgtagtacc gggttatcca tggtatgata tactattgtg cgattgtaat
    22861 gacagtgtga agacaacagg tacaaattgc aaggttttta taagctggtg agatgagtgg
    22921 aaatattttc acggagagag cgcggcgagt tttgctaaat cataaattaa aaattatttt
    22981 acttgaagta cataaccatc aaattcttat ttctaaacca acagaaaggg aaggatagcc
    23041 gtacaggagg tacgtcttca taaggtcgcg tgcgtgcttc gataaactca gcaaccttgg
    23101 atcaacggga ggtcactgag cttgttgaag tgctcaccaa cctatacttt ggcaggatgg
    23161 cttccacagc acgctgctaa aagggtagcc atatagcaaa ttagaaggct tttttctctg
    23221 gcactttctt ttgtagccgc ctggacaaaa gaaagtgcat aaagagtatt caaaaatatc
    23281 ttgaaaaaga aacaactcca tgccattctg tgatgtaaag aaatatcaaa tcacatttct
    23341 aaaattaaca ataagcggtg taagtctatg cgcaaaacaa agatagtatg taccattgga
    23401 ccaacaagtg aatctaaaga aatgataata caacttgtca aagctggcat gaacgtggca
    23461 cggcttaaca tgtcacattc aacacaagag ttgcatgcac agcgcattgc actcattcgt
    23521 gaagtgtcaa aagagttgaa cgtccccatt ggtattttag ctgatctgca gggaccaaaa
    23581 attcgcattg gcacattgaa agaaaaagta atgctggagc cgggtcagca gtttatcctt
    23641 accacacgaa gtgtgccggg caattcagtt gctgtcagtg tctcgttaaa agaacttcca
    23701 gaatcggtaa acaaggggca aaccatactt attgacgatg gcttattaga attgcgtgtt
    23761 gacgaggttg aaaacactga tatctacact acagttatac gtggtggtga actgaaagac
    23821 aacaagggta ttaatcttcc caatgcttcc ataaaaacca gttctattac acaaaaggac
    23881 attagtgatt tagaatttgc cataaaacat gatgttgaca tgatagcact gtcgtttgtg
    23941 cgcagcgctc atgatgtcat acagcttaga agcatcatgg aagagtttgg cggaacatta
    24001 cccatcattt caaagattga aaagcatgaa gcagttgcca atattgacga cattatcgct
    24061 gcttcgcagg ggattatggt ggcgcgtggt gatctgggta tagaaatccc cattgaacag
    24121 gtaccgatag tccagaagat gattattgaa aaatgtaacc agcaggctat tccggtgatt
    24181 actgcaacgc agatgcttga ttcaatgata cgtaatccca taccaacacg ggcagaggca
    24241 accgatgtgg ccaatgcagt atttgatgga acagatgcac tcatgctttc cggagaaaca
    24301 gcatttggtc agtatccggt agaagccgtg caaaccatgg cacgtattgc tgaatatgct
    24361 gaaaagactg ttatatataa agcaacaatt gcagaaaaaa agatctatac aaaatccact
    24421 ataaccgatg ctattgcact gtcagcgcat gaatcagcaa aaacgttagg tgccgatacc
    24481 atccttactg ccacacagtc aggatatact gcacgcaaga tttccaagta taaaccgcag
    24541 ataccggtaa tagcagtaac aaccaatcac aaggtggtaa atcagttagc gttatcgtat
    24601 ggggttatcc cactgcagat aaaacagtgc aacgatatta acgaactggt tgatgaagca
    24661 atagcaatag gcagggtaaa cggtcttgtt catgatggcg atctggttgt tatcactgca
    24721 gggattctgg cgggtgtaac cggtggaaca aacgtcatgc gtatatatgt ggtagcccag
    24781 gagatagcca gaggaacagg atttggcgaa ggggtggttc gtggtatcat aggaacaagc
    24841 caatccgtag aactatcgcc caatactatt attgtggctg atgacattgc gcatattaaa
    24901 ggcacaaagc caagagccgt gataaccggt gaatgcagca tcagtgcgcc catcattcat
    24961 tactgcaggg agaatgaaat acctgtcatt ggtgggatta ctattaatga aatcaacctg
    25021 aaacagggcc aggaagtaac gcttgatatt atgcggggaa ccatttataa cggaactgtc
    25081 catttgcctt ttgagtaatc tatgattgaa gaaattgaaa ataccgaaac agtgtattcc
    25141 gtaagcgata ttactggaat actgaaacag ctcattgagt caacgcctga gcttaatggt
    25201 gtgtgggtaa agggtgaaat ttctaacctt acgtaccatt cgtcagggca catctatttt
    25261 tccctgaaag atgaaggggc cgtaattcag gctgtttttt ttaaacatgc aaacaagcat
    25321 ctggatttca agcttgaaga aggcatgagt gtggtggtgt ttggcggcgt tacggtgttt
    25381 gaaaagcgcg gttcatatca atttattgtg tataaaatcc gcaaagaagg cctgggtgaa
    25441 ctgttacaga agattgaaaa gcttaaggaa aaattacata aagaagggtt gtttgcgcct
    25501 gaacgaaaaa aaccgcttcc agcactaccc aggcgtattg gtgtggtgac atcgccaaca
    25561 ggtgctgcca tccgtgatat cataaaggta gcaatgcgtc gcttccccaa tattgaaatt
    25621 atccttgcac ctgcaaaggt acagggtgaa ggggcagttg agtcgatagt taccggtata
    25681 caggaactga ataatccgca gtggggagtg gatgtcataa ttgccggccg tggcggaggt
    25741 tcatttgagg atttgatggc atttaacgaa gaagccgtgg ttagagcatt tgccggctcc
    25801 agagtgccta ttatttccgc agttgggcac cagattgacc atccattaag cgatttagca
    25861 gcagatgttg ccgcacccac accatcagca gcagctgaga ttgccgtgcc ggtaaaagca
    25921 gaacttatag ctgctattga caattacacc atgcgtatga tgcggcggct taccatgatg
    25981 gtacagtcgt ttgctaatcg cataatgcta attgtcaggc gcagggtttt tcaaaatccc
    26041 atgagcctgg ttgaaaacta tgagctagcg ctggctgacg ctgaaaacag gatacaggcg
    26101 ttgacgcaca aatgtatcac ggatacaaaa gaacggttaa gtcgtgtaag ggatttagag
    26161 cttattataa aaaatagatt gcaaaactac tggcacatct ttatgatgaa tgcaagcaaa
    26221 ctggagcacc tttcaccggt aagtgtgctg aagcggggtt attccattac actgaaagat
    26281 tcacagatag tttcttcaat aaagaagatc caccagggtg atactatcag ggttattgtc
    26341 tatgatgggt caatgcaatg tgatatacaa ttggtaaatg aagaggtgag ctttggaaaa
    26401 aaaggctaag aaaataacct ttgaggatgc gctggaagag cttgaggcaa tagcatcaaa
    26461 gttagagtct ggtgaactga cgctggagca gtccatacag tactatgaaa ggggtgttga
    26521 actggcaaag ttttgccggc aaaaattaga agaagcagaa cgcaagattg aggtgttgca
    26581 aaagcagcct gatggaacgg tacaaaaaaa atcggttaaa gttaaagagg atacagggga
    26641 aatagaagat gatgaggata tacagggttc acttctataa gttgttgctg tgtgtggctg
    26701 ttatcagcat ggttacctgt ggcaaagaag caccactgca gttttgtgaa ggtgttgata
    26761 aggatggcaa acccgttcat tgcggaaaag aatttaccac aggtgatatg acgctgtatg
    26821 ttatggcaca ggagcctttt ggaagtaata cagcaacggt aatcgtgtat ggaaaaaaga
    26881 aatacagcca gcctgagtat acccgctttg attataccat aaatcctgat ggtaatactg
    26941 ctacaatacc attgtccctg tatgctgagg gtacgtatat agtgaaagta atagtcaaag
    27001 ataaagaaat agcaagtggt gaaattactg taattgatac gtattaatta attgtattta
    27061 ttttgagctt tccgtttttt ctcttcaata agctggtgga taacaagaag tgttgcacct
    27121 accacaattg aagaatcagc aatattaaat gctggccagc gaaaaacatc atcactacca
    27181 atgtagataa aatcaaccac cccgggttta ccggtaatcc tgtctaaaat attgcctatg
    27241 gcgcctgcgc agataagtcc catgcttaca gtaaagataa gcgttttgtt cttttcaagt
    27301 ataaagtatg caacaagaag caaaaacaca ataattgaaa taataaggaa aaactgctga
    27361 tatccctgta gtataccaaa tatcccaccc tgattgtaga taagtgtcag ttgtacaaag
    27421 ctacccagca cattgacccg ttcatacata gaaatatatt tttccacaag cgcttttgtt
    27481 ataatgtcgc ttcctaaaat aagaataaaa atcaacactg caatcaatgt gttttttgtt
    27541 tcttgtttca ttgtggtatc cttacagctt caatacaata tcggtgcatc gtttgcaaag
    27601 ttccgggtga tccgggtgtg aaccaatgtc gtgcgaataa ttccagcacc tgacacattt
    27661 tttccctgtt gatttaacta ccagtatgga gctactgtca taatcggcca tgccatccag
    27721 ttttttatct tcaatgtgca cttcggccac ctggaaaaag cggttactat cgctcattga
    27781 ctgcaaaagc tcttttgatg acgcacctgc aatatatatg tgaacatccg cctcaagcga
    27841 gctaccaatt ttcttttcct ttcgagctat ctctaaagcc ttcaatacat ctttcttgat
    27901 gtcaatgaga gcatccatct tctttgccag tgtttcgttg tgccattccg caggcagttc
    27961 actgtataca tcagcatgca cggaagattc tttgcctaaa aattgccata tttcttcggc
    28021 agtgaacacc agtaccggtg caatgagtcg aagcagtgtt tcggtaacat ggtacaggac
    28081 agtctgattt gctcttcgca atttggaatc ctttgcttct acataaagga tgtcttttga
    28141 aatatcaaaa tacagtgatg aaaggtccac cgtacagaag ttcagtattc ggcggtacac
    28201 aaggtgaaat tcatactgtt catagtgctg gcgcacctga ttggaaaggg tatagagttt
    28261 atgcagtatc catttatcag tgtccgatag ctcatcgtaa tggacacact gtgaaggatt
    28321 aaaatcagca cagttcccta tcatgaattt aaaggtgttg cggattttac ggtatgaatc
    28381 tgcaatttgc tgtatcatat cgtaaccaat cctgacatca ttgcgatagt cctcggaagc
    28441 aacccacaga cgcaggatat cggcaccaaa cttgttgatt atatcatcag gcgggataac
    28501 attaccaagg gatttactca ttgcccgccc ctgatcgtct aacatgaaac cgtgagtcag
    28561 tacggtatta tatggagccc gctggcgcag tgcaagtgct ggccacaatg aagactggaa
    28621 ccagcctcga tgctggtcgc ttccctctaa atacaggtcg gatggccagc ggtggtcatc
    28681 ccggctgtca agcactgcaa aatgtgaaac gcctgagtca aaccatacat ccagaatatc
    28741 atattctttt tcaaaatcgt cgcttccgca ttcgcaggta aagccttcag gaataagact
    28801 gtgtatctca tcggtatacc atgattcaat gctgcgctct cgagcaatct ttgcaaaata
    28861 aaagatggtt tcagcattca tctggttttt tccacatttt ttacagtaga aggaaggaat
    28921 gggaaccccc catgaacgct gccgtgaaag gcaccagtca gggcgtgttt ctaccatgct
    28981 tttaaaccgc aatttacccc attcagggat ccactgtaca tcttcagtta cctgtaaagc
    29041 taactgtcgc atatcattat ggtcaatgag taaaaaccac tgagcggtag ccctgaagat
    29101 gagcggctgt ttgcagcgcc agcagtgggg atatgaatgt tcaatatcat tggtaaaaat
    29161 cagtacattt ttttctttta aaagatctat tatttttggg ttagcatcaa agacattgac
    29221 gcctttcatg agggcaaatt catcggtaaa tttaccttca tcatcaaccg ggcagaatac
    29281 atcaagcccg tattgaagac ctacaatgta gtcctcaagt ccatgccccg gggcaatatg
    29341 gacaatgccg gtaccctgat ccagtgtaac aaaatcgcca aagatgactt ttgattccct
    29401 gtcaataaaa ggatggtata ctttcagtga gcgtatgatg tcaattggta agggtatctt
    29461 ttcacctaag gtttttccag ttatggcgga aaatgattcc gcaagtccat ctgccataat
    29521 taaatactcg ttacctgacg tataagctga gtaatcaaaa tctggatgaa aacaaacagc
    29581 caggttggca ggtaacgtcc atggtgttgt ggtccatacc accacataca ggttgttagc
    29641 atcaacccct ttgaatgtta cggatgtggg atccacttta aacttaacaa aaattgacgg
    29701 tgatgagtga tcatgatatt caacttcggc ttcggccagt gctgttacac aggttgggca
    29761 ccagtaaata ggttttttcc ctttagtaat aaattcccgt tcaaaaagcg acccaaaaat
    29821 ttccacaatg gtggcttcat aatccagtga catggttaaa tagggattat caaactcgcc
    29881 aaaaaccccc aggcgtttaa attcttctcg ttgaatatca ataaatttct gtgcatagtc
    29941 cctgcacagc cttctgattt cctgttttgg gagctttttg gctttatcac ccagttcttt
    30001 tgtaacctgc agttcaatag gcagaccatg acaatcccat ccaggcacaa atggggcttt
    30061 aaacccactc attgtcttgt gctttacaat gatgtctttg agaatcttat ttaatgcatg
    30121 ccccaggtgt atgtgaccat tggcatacgg tggaccatca tgtaaaatgt ataattcatt
    30181 gccctcacgg gatttctgta tcagtttgta aatatcctct ttttcccatt tctgcaatat
    30241 ctgaggttca cgggaaggaa gatttgcctt catgggaaaa tcagaagcag gaagattaac
    30301 ggttttagaa taatccattg aaaccctcac acaaaaaata tcaaaatcaa tcccacgcaa
    30361 cgtcatggga cagtatatat ggtgctatct cactataagg aataaatact cgtgatattt
    30421 caaaaataca tttgtcaata gaattttact attttatgaa tacattttaa tgatttttaa
    30481 accacactgt ggtattaata cactatcgca tacaaatggt agtattgtat atataaattt
    30541 aaatttattt ttattttttt ggcgatagtt catgataaaa aattttatta tatactagaa
    30601 gaggataatc caatgattga gcttgatttc accaagttgg atgggctggt accagcagtg
    30661 gcgcaggatt ataaaaccgg cgaaattttg atggttgcat ttatgaacaa agaagcgttt
    30721 gaactgacat tgaaaacagg catagtccat tactggagca ggtcacgaaa gcaattgtgg
    30781 aaaaaaggcg agtcttctgg caacgtgcag gaagtaaaag aaatacgcat tgattgtgat
    30841 aacgattgtg tgttactaaa aattaatcag ataggcgatg cagcatgcca taccggttat
    30901 cgtagctgct tttacagggt tgttgaaggc ggtgatctaa aagtagatgg tataaaaatc
    30961 tttaatcctg aagataaata tggagataaa aaatgagcct tatcaagtta ggaattccta
    31021 aaggtagttt agaaagtgca acaatagaac tttttaagcg ggcaggtttt tcaatatcag
    31081 tatcatcacg gaattacttc cccagcattg atgaccccga gattagctgt tcccttgtcc
    31141 gtgcacagga gatgtcaaaa tatgtggaaa gcggcgtact tgatgttggg ctaaccggcc
    31201 ttgactggat actggaaaac gagtctgatg ttgaaataat atctgacctg atttattcca
    31261 aggttagcac tcagaaggca aaatgggttt tagcggtacc caacgattcg cccatcaaga
    31321 cattggaaga ctgtgcaggc aagaccatat caacagagct tgttaacttt accaaaaaat
    31381 attttgctga ccgcaatatc ccggtaaagg ttgaattttc atggggtgca actgaagcca
    31441 aggtggttga gggcctggtg gatgccattg ttgaggttac tgaaacgggt tccacaataa
    31501 gagcacacgg ccttaagatt atttacacat tgcttgaaac caataccaaa cttattgcca
    31561 acaaacagag catgaaggat tcagccaagc gcgcaaaaat caaacagatt gcgctgcttt
    31621 tgcgaggtgc cctgaatgcc aataatcttg tgggcataaa aatgaatgtc cctgaagata
    31681 agttagctga tgttgttaag ctgataccaa gcttaacttc acccacggta tccaaactgt
    31741 ataatgctac atggttttca gtggaaagcg ttattcagga atcggtggtc agggatctta
    31801 tccctaaact cattaatgcc ggagctgatg gaataattga atacccgctc aataaagttg
    31861 tgtcacagga tgatgtgtgc ttaaagtaac ttgttaaagg gtaatgcatg cagcatgatg
    31921 taatcagcag gggaaggcag tatataaagt cctttgctgt aacactgcct gccccacggt
    31981 tttatgccga tagttactgg tatttagcca ttgcaaaaaa aatatcaaca gtttcatcag
    32041 tcattgctta ctgcgctgga tgcatgcgtc aaaaagggag cgcattaggg cacagctatt
    32101 accatgcaga aaaagtagca attgaatcgg cggcgatagt tcttaaggaa aagggtattt
    32161 ccagccagtc aatacacctt gcaatgctgg cactcattgc aggatttctg catgattatt
    32221 accgggaaaa aaaagaccat ccggcaaaag ctgcagagta tgtacaggca catctatctc
    32281 acttcatgcc tgaaagtgat attgatgcca tcagttttgc catacgcaat cacgaagcat
    32341 ttaaagagta ccagatggtg gataacagtg atatcatgct gctatcaaat gcactgtatg
    32401 atgctgataa attcaggtgg ggccccgata acttcatata cacaatatgg gacatgattg
    32461 ctaccatgaa tattcccatc caggatatta ttgcccattt ccccggaggt gttgcacata
    32521 tcaatagcat tcgttataca ttcagatcca aaacaggcat ggattacggc cctgaattca
    32581 ttgatcaggg tatgctgata gctgaaaaat tgttacagtt tttacagaac aattcataaa
    32641 tttgattgtt ataaggatta taaaatgtat acgtgctgta atagttataa gtaataaata
    32701 cagtgaataa attgtaaaaa atcttgacaa ataacacctt tttttaggaa tattttatta
    32761 aaattgtata tattgtatat ttcagacagg cccacgtagc tcagtcggca gagcatttgc
    32821 atggtaagca aaaggtcacc agttcgattc tggtcgtggg ctcattatta attttataaa
    32881 aatttttatt tttttttgat tcaggtaatt aattacttga cgtggtgtgc tatatttata
    32941 gcgtgctttc gtatctataa aattgcatac aaacgctaaa aaactgtaaa aattcaatac
    33001 acttatacaa actattatta ataataggat atcattatgc gtgaagtagt actattatca
    33061 tgtaaagatt gtaaaaatcg caattatgca accaagaagg ataagaaaaa gcaaaccggc
    33121 aagcttgaag tcatgaaata ctgtaagcat tgcaataagc atacattgca taaagaaact
    33181 aaagcataag gtgcttaacg tgtttaataa aacagtacaa tttgttaagg aatccagaga
    33241 cgagttaaaa aaagtttcct ggcccgaacg tgatgaggta agtgctttga caatggttgt
    33301 tgttataacg gttattatca ctgcattgtt tctatggctt gttgatgctg ctttgatgaa
    33361 aatagtatct ctggtgatgc aatagctatg acaaaagggt ggtatgtaat acatacatat
    33421 tctggccatg agaataaagt taaactggcg ctggaaaaaa aagtccagaa tcttaacctg
    33481 ggggataaga tatttcaggt aaagattcca actgttgaag aacattcagt aaaagatgga
    33541 aagaaggttg taaagccaaa gaagatattt cccggatatg ttcttgttga aatggttctt
    33601 gatgatgaaa catggatggc aataaagagt attccggggg ttactaactt tgtaggttct
    33661 tttggtactg aaaaaccaag gccgctttct gaaaaggaag ttgatagcct tttcaataaa
    33721 atgggtgaag ttacaaccaa agacaaagtt gccgtggtca tggatttttc agttggcgaa
    33781 catgttaagg ttattgacgg cccgtttaaa gaattcaccg gtgttattga agaggtgtat
    33841 cctgataaag ggcgcttgcg tgttaaagtt gagatatttg gcaggggaac tcctgttgaa
    33901 ttagatttta ttcaggttgg taagatttaa ttaaaaaata ttgttttata gggttgatta
    33961 cgatggctcc aaaaaagaaa aaaatagtca ctcagattaa gttgcaaatt ccaggcggcc
    34021 aggcaaatcc tgcgccacca gttggtcctg ctttaggtca gcatggcctt aatataatgg
    34081 agttttgtaa agcattcaat gaaagaacaa aggatcagca gggaacaata ttgcctgtta
    34141 ttataactgt gtatgaagac aggacgtata cgtttatcat taaaactcca ccagctgctg
    34201 ttcttattaa aaaggcgttg aaacttgaaa aaggatccag cgagcctaac aaaaaaaagg
    34261 ttgggaaaat tactcaggct cagcttgaag agatagcaaa attaaaaatg ccggatctca
    34321 atgcgcatga tgttgaagct gcaaagaaga ttattgcagg tacagctcgc tctatgggag
    34381 tagaggtggt tgattgattt tttcactttg aaatttagga ttttttgggg ttgtatatga
    34441 agagaggaaa aagaataaca gacgcacgag caaaaataga taaaagcaaa ctctatccac
    34501 ttgaagaagc tttgaccatg atgaaagagt tggcttttgc aaaatttgat gagactgttg
    34561 aagtatcggt aaaagtcatt cataaaagct atcagaatgt gcgtggttcg gtagtattgc
    34621 cgcatggcac gggtaaagat attaaagtac ttgttatttg caaaggcgat aagcagaagg
    34681 aggctctgga agcgggggct gattttgttg gtgctgaaga tgctattgaa aaaattaaaa
    34741 atggatggct tgattttcag gcagttatag ccacgcccga tatgatgaaa gaggttggta
    34801 aattaggtcc catacttggg aaaagagggc ttatgccaaa accaaagtca ggtacagtga
    34861 ctgatgatgt gaaaactgct gttaaggaat taaaaggcgg aagagtagag tataaggctg
    34921 ataaaacagg agttattcat ttaggagttg ggaagctttc ttttgaaccg caaaaattag
    34981 ctgaaaatat taaagcattt tatagccagg ttgttaaaga caagccttct gatgcaaaag
    35041 gtaactatat acgttctttt catatatgct ctacaatggg gccgggaatc aaaattaatt
    35101 ataaggggat tgaacggtaa tggttaagca atataaggta gattttgtca atgagctaaa
    35161 ggaaaaatta gcttcaaaaa agaatgttat attaactaat tattcaggca ttaaagttaa
    35221 ggatatgtat cagctccgca agattttgcg tgaaaaggga gctgagtata aggttgttaa
    35281 aaatacttta ttcaggaaag cacttaaaga ttgtggttat cctgagatag atcagtatct
    35341 tgttgggcct attggcgttg tatttgtaga taaagaagtc agtgaagttg ccaaggtttt
    35401 aaaggatttt cagaaagagc aggataagtt ccagtacagc gtggcgataa tggacaacgt
    35461 tgtatattcg ccaaaagatg tgcagcgtat tgcagatctg ccgtcaaaag aagtattgtt
    35521 ggcacaggta atgtcgctta taaatggccc tgcatcaggt actgctatag ctatcaatca
    35581 gattatggca tcactggcac gcggtattaa gctggttgca gaaaaaaacg cataacacat
    35641 taatgtaagc atataatcaa gggtaaatga tactgagtaa agatgtgaaa agcagctttt
    35701 tagtattggt tacccttata ggtttttacg actaacgatc gtattaactt caagattcac
    35761 taagtataag gagtaacata atggcaaagt tatcagtaca ggaattgttg gaagcaattg
    35821 gtagtttaac acttgttgaa gcagctgaac ttgtaaaagc tatggaagaa aagtttggca
    35881 tttcagcagc ggcaccagtt gcagtagcag cagcaccagc agcaggcgct caagctgcag
    35941 aagttgagga aaagactgaa tttgatgtta tcctcacagg ttttggtgat aacaagatta
    36001 atgttatcaa ggaagtcagg gcaattactg gtttaggttt gaaagaagcc aaagacctgg
    36061 tagaagctgg tggcaaacca gtcaaggaaa aggtttcaaa ggaagaagcc gagaagatca
    36121 aaaaacagct tgaagaagct ggagcaactg tcgagattaa gtaacgtttt aaacgtaaaa
    36181 tatcttgaaa agattctggt aatactgtat attgaaaaca gtagtaggtt tggttgcgat
    36241 gcaatcaaac ctactgttgt tgatattggt gaaatttgtt aacagtttca ttcttttgta
    36301 tacaataaca gtgccccaaa cagtatgaat aaaacaataa acgagcaaaa tctttttaca
    36361 ataattttaa tttttaatcg attgtccgat tttcttcata ttttggctta ttgtgaataa
    36421 atcttatttt aggtgtaaac tatgcaaaaa caacaaaaag ttatccgttt tggtaaaata
    36481 aaacccggca ttggattgcc aaatttgatc gaaaatcaaa ccgaatcgtt taaatggttt
    36541 ttacagaagg atgtagcacc ttccaagagg aaaaaccagg gacttcaagc agtgtttctt
    36601 gaaacatttc caattgtaag ccccaatgat gatatagaac tggaatttgt tgaatatgag
    36661 ttaggtgagc caaaatataa tgtacaggaa tgtaaggaaa gagatgttac ctatgcagct
    36721 ccacttaaag caacaatacg gctcataaaa aaggatacca tggaagtccg tgagcagtct
    36781 gtgtatatgg gtgatatacc attaatgact gaaagcggca cttttattat aaatggcgca
    36841 gaacgtgttg tggtaaatca gttgcacagg tcaccgggaa tattcttttt ctgggatgaa
    36901 attgaaagaa tgtacagtgc ccgggtaatc cctgacaggg gctcatggct ggaatttgaa
    36961 atggatgcta aaggcctgat agttgcccgc attgacagaa agaaaaaatt ccctgcaact
    37021 attatactta aagcattagg gtataatact aatgaagaga taattaagct tttctatgat
    37081 acaaaaaaga taactcttgc tgaagaaaaa gattacgaat tcctgaaaaa taagcgggtt
    37141 gcttctgatg ttattaatcc agaaacagga gaagtactat tagaggctgg tgaacgaata
    37201 actattgact atattgatat actgcgtgat gagaagataa aagttgtaga agttatagaa
    37261 ttcccaaaga ataaagatga tgcatatctc attacatccc ttgaaaaaga cgattgcaag
    37321 agctctgaag aagcattgtt gaaattgcat cagatgctga gacctggtga accaaccaat
    37381 attgataatg cacgtgagat atttaatcga ttattctttg atcccagaac atacagtttg
    37441 gggggtgtgg gacgctataa gataaataaa aaatttggtt ttgacatgaa agaggtaaag
    37501 gaagattacc tcagaaaaga agatatcgta catacagtta aatatttaat aaaccttatt
    37561 gctgaagtgg atgggtatat cgttgatgat attgaccatt tagggaacag aagaatacgg
    37621 cccgttggcg agctaatcca gaatcagata aagattggat tccagcgaat ggagcgcgta
    37681 attaaagaac gtatgactat acaggatgtt gatgtggtca caccacaggc tctcataagt
    37741 attaagccaa ttactgctgt tattaatgaa ttttttggct ccagccagct atcgcagttt
    37801 atggaccaga caaacccgtt ggcagagtta acccataaac ggcggcttaa tgcactgggg
    37861 ccaggtggtt taacacgtga gcgggctgga tttgaagtgc gtgatgttca cccatcacac
    37921 tatggaagaa tgtgccccat agagacacct gaaggttcca atattggatt gattatatca
    37981 atgagtacgt atgcacgcat taatgattat ggttttcttg agacccccta taaggttgtt
    38041 gaaaatggta gggttactga caaggttgta tatcttactg ctgatgaaga ggataagtat
    38101 tttatagctc aggccaacgc taaaatagat gaaaatggca agtttgttga taatcttatc
    38161 ccctgccgtc accgtggtga ttttccctat aaacggcctg aagaaataca gtatatggac
    38221 gtatcgccgg atcaggtgtt ttcagtttca acatgcctca ttccgttcct ggaacatgat
    38281 gatgctaacc gcgcactcat gggttcaaat atgcagcgcc aggcagtgcc acttttaacg
    38341 gaagaagttc ctctggtagg caccggtatg gaaggccctg ctgctatgga ttcaggtgtt
    38401 gtggttaagg caaaacgtgc tggtgaagta atctatgcag atgctaaagt tgtcagggtg
    38461 aaaacatcca gcggcgaggt agatgaatat actttgcaaa aatttaaacg aaccaatcag
    38521 ggtacctgtt tcaaccagaa accaattgtg cgtaaagggc agaaagtaaa agtgggtgac
    38581 atcctggcag atgggccagc cacagacaat gggaaactgg cattaggtaa aaatatcctg
    38641 gttgctttca tgccatggat gggatataac tttgaggatg caattctgct ttccgaacgg
    38701 ctggtatatg atgatgtata tacatcacta catattgaac agtttgagat agaagccaga
    38761 gagaccaagc ttgggaggga agtaatcacc cgagatattc ccaatcttag cgaaaaagcg
    38821 ttcagagatc ttgatgaaaa tggtatagta cgaatgggtg cctatgttgc acctggcgat
    38881 atacttgtag gtaaggttac cccaaaaggg gaacaggacc ttacacctga atataaattg
    38941 ctgcattcaa tatttggtga aaaagcgcgt gaggtgcgag atacatcgct cagagtacct
    39001 aatggcgttg aggggattgt gattgatgtg aagcgttttt cccgtgagaa tggtgatgag
    39061 ttatctgcgg gtgtagaaga gcttgtcaaa gtgtatattg cttcaaaaag gaaaatatcc
    39121 gttggtgaca agatggctag ccgccatggg aacaaaggtg ttatatcaag gatagttcct
    39181 gtgtatgata tgccgtattt gccagacgga acgccagttg acattgtttt aaatccgcta
    39241 tcggttccat cacgaatgaa tttaggacag cttcttgaaa ctgaattggg ctgggcggca
    39301 aaggaactgg gatttatagc agaatgcccc atatttgata gtcctgaaga gcaagaaata
    39361 cgcgagcttt tgaaaaaggc aggtttgcca gaaacttcaa agactatcct gtataatggt
    39421 cagacaggtg agccctttga aagtgaagtt atggttggtt acatctacat gttgaaatta
    39481 gctcacatgg ttgatgataa gattcatgca cgttcaacag gtccatattc actggtaaca
    39541 caacagccat tgggtggtaa agcacagttt ggcggacaga gattgggcga gatggaagta
    39601 tgggcacttg aagcatacgg tgctgcatac acattgcagg aactcctcac agtgaaatct
    39661 gacgatatgt tagggcgagc acggatttat gaagcgatag ttaaaggtat taacaccact
    39721 tcgcctggaa ttccggaatc tttcaatgta ttggttcagg aattgcgggg ccttgcactg
    39781 gatgtcagaa tttacgatga aaatggtaat gagataaatc tctctgaatg gagcgaaggc
    39841 ttaagtaagc caaagagaaa aatcaagctt gaaacgcttg aaaatgagta atctgtatat
    39901 tttaaaaaat tttaattaag gtaagaaggt aatacgatga gagattttaa cgatttcaat
    39961 tctatccaaa taaaattagc ttctcctgat atgatccgtg agtggtcata tggtgaagtg
    40021 aaaaaacctg aaacaattaa ctatcgcacc ttaaaacctg aaagggatgg ccttttttgt
    40081 gaacgaatat ttgggacaac aaaagaatgg gaatgctatt gcggcaagtt caagtctata
    40141 cgatataaag gtgtagtatg cgaccgttgt ggcgtggagg ttacacatta taaggtcagg
    40201 cgtgagcgtg gtggtcatat tgaattagca tgcccggtag cgcatatatg gtattatcgc
    40261 tcagtaccat cacggatagg gttgttgctt aatttgacag taaatgagtt aaaatctatt
    40321 ctgtattacg aaaaatatgt tgttattgac cctgctgagt ctgatttaaa atatggtgat
    40381 gtgatagatg aagatacgtt caaccattat tatgaaacat acggcgatga atttgtagcc
    40441 gatatgggtg cagctgcaat caaggaaatg ttgagaagaa tagaccttga tggcgaagca
    40501 cgtaaattgc gaagcaaaat tgcaggtcag gataaactga aggtagataa aaaagatatt
    40561 aaacgacttg agcttataga agctttcagg gattcaggca accggcctga gtggatgata
    40621 cttgatgttg taccggttat tcctccggaa ctgaggccaa tggttcagct tgatggtgga
    40681 aggtttgcaa catctgatct taatgacctt tacaggcgag ttattaacag aaataaccgg
    40741 ttaaaacgat tgctgcttct gagagcacct gatattatag taagaaatga aaaacgtatg
    40801 ctccaggaag cggtggatgc actgtttgat aacagcaagc gcaaacgcgc agtgaaaggc
    40861 aaagccaaca ggcctcttaa gtcactcagt gacatgttaa aaggcaaaca ggggcggttt
    40921 agacagaacc ttctgggtaa gcgagttgat tattccgggc gttcagttat tgttattggc
    40981 cctgaactta aactgcatca gtgtggattg ccaaagaaga tggctgtgga attatttaag
    41041 ccatttatca tgaagagact ggtagataaa ggttttgtac agaatataaa atctgccaaa
    41101 aagatggtgg aaaatgaaag gcctgaagtg tgggaagttt tagaagaggt tattgccgaa
    41161 catccggtac tgttaaaccg tgctcccaca ctacaccggt tgggaattca ggcgtttgaa
    41221 ccagtactcg tagaggggaa ggctatcaag ctgcatccat tggtatgcca tgcatacaat
    41281 gctgactttg atggcgacca gatggctgtt catgtcccac tatcgcctcg agcccagata
    41341 gagtgctgga cattaatgct ttcagcaaac aatttgctta accccgcaaa tggtcaacct
    41401 atcgtaactc caacccagga tattgttctt gggatatatt atttaacctc tgaattaaag
    41461 agtgaaagtg aaaatattcc gtattttggt tcaatggatg aggtgctcta tgctcttgaa
    41521 ttcagacaga taaagtacag aacaaagatt aaatatctga tggatggtga atttattgag
    41581 acaacagcag gcaggttaat ctttaacgag aaacttccag caggctaccc ttttgttaac
    41641 tatactgtca atgataagga actttcaaaa attatttcta aaatcttcag agagtttggg
    41701 ccaactgcaa cagtaaaggt gcttgatgac atcaaggaat tgggctataa gtatgcaaca
    41761 tattttgggc caactatctc aacaagtgat gtaaaagtgc ctccattgaa ggatgagttg
    41821 ataaaaaaat ctaatgagat gctggaaaag attgagaatg aataccgtaa cggttatata
    41881 accaatgagg agcggtataa tcgtgttatt gatttatgga caaaaaccaa tgaagatgtt
    41941 actgaggtaa tgttcaatac catggctcag gatgctgaag gatttaatcc catttatatt
    42001 atggcacagt ccggagcacg aggcagtaaa cagcagatac ggcagcttgc aggtatgcgt
    42061 ggtcttatgg caaaacccag cggtgaaatt attgatgtcc ccatcaagtc aaattttaaa
    42121 gaagggctga cagtacttga atattttatc tccactaacg gtgcacgaaa aggcttggca
    42181 gatactgcat taaaaactgc tgatgccggg tatttaacac gaaggcttgt tgatatagcc
    42241 caggatgttg ttataacaat ggatgattgc ggtacctcag taggaattga tgtgggtgca
    42301 ttaaaagaag gtgatgaagt tgtggaatct attggttcaa gggtactggg gagaaccttg
    42361 ctttatgatc tgtatcatcc ggttaccaat gaagttattt gcaagagtgg tgaattaatt
    42421 actgaagaga ttgcagaaaa gatagatgaa ttgggccttg atcgtataac aatcagatcg
    42481 gtgttaacgt gtgaagctcg acatggtgtt tgtgcacgct gttatggcag aaatttagcc
    42541 actgcacgta tggttgatat tggtgaagct gtagggatta ttgcagcaca atctattggt
    42601 cagccaggta cacagcttac catgcgtacc ttccacattg gtggtactgc atctcacgtt
    42661 gttgaagaaa gtgaaatcag ttttaattac cctgtctatg taaaggaaac taccttcaaa
    42721 acagtaaaaa ctgaagatga acagggtgag aagaaaacca tatctgtacg gcgtggttat
    42781 atcgttttac agcgattact ggaagagata cctgtaaaaa gtggtgaact ggtggtaaat
    42841 gatggtgaca gagtatatag cggaacacca atagtaaaaa aatctgatgg gtcatttatt
    42901 gtcacaacaa aatcaggtgt ggttaaactt gataaatcca gggcatatat ccttggagag
    42961 cccaatatga ttcctgttaa tataggttct accatttatt taaaaactga taaattttat
    43021 catgtaaatc cacagacggg ggaaactgat gtactggtga aatttgatcc ctggtttgag
    43081 ccaatattaa ctgaaataaa tgggaaggtg aaattcattg atattgaaat caataagagt
    43141 atgagggaag agatagatcc tcagacaaat cagctcaagc gtattatcgt tgaagcaaag
    43201 acggaaaagt tgcagccagc catagaagtt tcatctgctg atgaaacctt caactacctg
    43261 ttgccaaaag gtgctcacct gtttgttgat gatggacaga cagtaaaagc aggtatggtt
    43321 ctggcaaaaa taccaaaaga agttgaaaag acaaaagata ttacgggtgg cttgccacga
    43381 gtagcagaac tctttgaagc acgtaccccc aaggataagg caaccatatc agagattgac
    43441 gggcgtgtca aaattggcga taccgtcaag aataaacgta agataattgt cactggtgat
    43501 gatggcgaag aaagagaata tcagatacct gcttccaagt atttacgggt tcaggatggc
    43561 gactggatca ataaaggtga taaaattgat gatggcccaa ttgatccgca tgatatcatt
    43621 gatattaaag gaccgcgcga acttgaaaaa ttccttgtaa acgaaattca ggaagtgtat
    43681 cgtttgcaag gtgttcagat aaatgataag cacattgaga taattgtgcg acagatgtta
    43741 cggaaagtca tcatagaaga tcctggtgat acaagctttg tcgtagacca gatagttgat
    43801 aagttcgaat ttgatgatga aaatatgcgt gttgaaaaag aaggtggtaa agcagcatct
    43861 gcaaaaccgg tactgatggg tataacaaaa tcagctctta atacggaatc gtttatatct
    43921 gctgcatcgt tccaggaaac aacccgcgtg ttaactgaag cggcaattaa aggaaagatt
    43981 gatagattac gtggattaaa ggaaaatgta atcattggtc atttgattcc ggcaggtact
    44041 ggcgtacgtg agtacaataa ggttactgta taccaggaag ttaagggcga cttagatggc
    44101 gttgcaaagg aagtagagga ggaaatccct gctgagaaat aaaatttctt gacaattcta
    44161 caggtaaaat tgattaatca ctttcaatta aaattttgaa agtgattaat ctgtataata
    44221 taatgttata atattcaata aatgtagtga ggagacagga tgcctacaat taatcagtta
    44281 ataaggaaag ggagatctga aaaggttaag aaatcaaaat ccccggcact gaagagttgc
    44341 cctcagaaaa gaggagtatg tacacgagta atgactgtta cgccaaaaaa acctaattca
    44401 gcattgcgga aggttgctcg tgtaagacta accaatggaa tagaagttac agcatatatt
    44461 ccaggtatag gccataatct tcaggaacac tctgctgtgt tagttcgagg tggaagggta
    44521 aaggacctgc caggtgtgag gtaccatata gtacgtggga cattggattc tttaggagtt
    44581 aatgaccgca aaaaaggaag atcaaaatac ggcaccaagc ggccaaaagc ataatgtaat
    44641 aattatttgg tatagtgagg tttaataatg gcacgaaggc gtagaccagc aaaaaggaca
    44701 attttaccgg acccaaaata tggcaatata gtagtttcaa aatttataaa ttgcatgatg
    44761 ttaaaaggca aaaaaggcgt tgctgagcag gctttttata aagcattgga actcattggc
    44821 gagcgtactc agaaagaccc aattgaagta tttattactt cgcttgaaaa tgtaaagcct
    44881 cttgttgagg ttaaatcacg acgtgttggt ggtgcaacgt atcaggtacc tgttgaaata
    44941 cggccagaaa ggcgccaggc acttgctatc aggtggctta taaacaatgc ccgcagcaga
    45001 aatgaaaaga ccatgtttca gaaactggcg ggagaactac tggatgcata caacaacacc
    45061 ggtgcttcaa taaagaaaaa ggaagacact cacaagatgg cagaagctaa caaggcattt
    45121 gcacattata agtggtaatt atagtattat ttgctgttta aaagtttaat atataaaata
    45181 ccagggcatc cccctggtat ttgatgcttt atcataaata attaatttta tcatcaggta
    45241 ttatgggaag agagttacca ttacacagga cacgaaatat tgggataatg gctcatattg
    45301 atgcaggtaa aaccacatta accgagcgca tcctctatta tactggcaag acctataaaa
    45361 ttggagaagt ccatgatggc actgcagaga tggactggat gattcaggaa aaggagcgag
    45421 ggattaccat cactgctgct gctaccacaa cctactggaa aaatacgcgt atcaatatca
    45481 tagacactcc gggacatgtt gattttaccg ttgaggtgga acgaagtctg agagttttag
    45541 acggagctat agccgtattt gatgccgttg ccggagtgga gcctcagagt gaaacagtat
    45601 ggcgacaggc tacacactac aatgtaccca gaatatgttt tgtaaataaa atggacagag
    45661 tgggagcaga ttttgagaat tgtctcacca tgataaagga acgcctgtat tccataccgc
    45721 tgcccttaca aattccctat gggaaggaag atagcttcaa aggtgttatt gatgtagtgc
    45781 agatgaaagc actggtgtgg aatgaccagt taggtgagca ctatgacgtt gttccaatac
    45841 ctgaagaata ccatgatgaa gctttgtatt atcgtgaaca tctgattgaa ctggttgcag
    45901 aatacgatga ggaaatgctg gaaaaatatc tggaaggtgt ggaaataacg gaaactgaac
    45961 tgaaacgggc catacgcaat gcaacgttga aaacagcatt gtttccggta ttttgtggaa
    46021 gcgcgctgaa aaataaaggt gtacagcctt taattgatgc cattactgat tatctgcctt
    46081 cgcctaaaga cattccggct atcagaggac atgatgtaca tcatgaagat acaataatgg
    46141 agcgtcatcc ggatgacaag gagccattct gtgccctggt gtttaaaatt gcaagcgacc
    46201 cgtatgtggg caagctctgc tatatcaggg tttattcagg gcaccttgca gctggcgata
    46261 ctgtcctgaa tgttgacaag cagaagaagg aacgtattgg tcggatactt agaatgcatg
    46321 ccaatgacag ggaagacata aaggaaatat ataccggaga tatagccgcg cttgtaggtt
    46381 tgcggtttgc ccagacaggc gatacgatta ctgctgttga tgcgccaata cttcttgaaa
    46441 agatggattt ccctgagccg gtgatagcac gtgcggttga acctaaaacc agggctgacc
    46501 tggaaaaatt acatacggtg ctcaagcgct tggaagaaga agatccgaca ttttttgtta
    46561 aaattgatga ggaaagcggc caaaccatca tatcaggcat gggtgagttg catctggaaa
    46621 ttattgttga taggctgcag cgcgagttca atgtgcttgt caatgttggt aaacctcagg
    46681 ttgcctacaa ggagacagta acagcacatg cacaggctga aggagtattt gaaaaagaga
    46741 ttgccggtaa actgcagtat ggtcatgttc tgcttgggat aagtgcgtgc gatagctcct
    46801 ttgaatttgt ctcaaaggtg gattccacag tggtacccga aaattgcata aaagctgtgg
    46861 agatgggttg ccgtgatgcg ctcagttccg gggtaatagc cggctacgag atgaccaagg
    46921 tacgggttgt tcttgaggac gccaagtacc gtgaggatgc ctcggttgat catgcatacc
    46981 ggattgctgc aacaatggct ttcagagatg cagcacggaa agcctgccct atgttgcttg
    47041 agccgattat gaagcttgaa gttgtagtac cggatgaata tatgggtgac ataatcaatg
    47101 acataaactc acgtcgcgga aaaattgaac atattgatat ccgtggtatg ttaaaggtca
    47161 tcgatgcgta tgttccgctt gctgaagcat ttggctatgc aacagctgta cggtcgctta
    47221 gtcagggaag ggcatcgcat acattacagt ttagccatta tgaagttgta ccgcaggctg
    47281 taatggaaaa ggttcttggc agttactatc gccctattta gaacataatg atggtaaaaa
    47341 attaatttta atttttataa aaaaatattt gatattttat aaattacgca ataaattagt
    47401 tgacagataa agcatatatt tttttaattc acacacttcc actgctatgc accaaaatag
    47461 atgaagtgga agtttgtgtt atctaaacgt aatactttct aacgttcata gagtgattaa
    47521 cctttaggta ttttatttcg tttatgttgt atggttgtat ctgtgtgata taatgaaatt
    47581 tattattttt aacaaggaga taacaaatgg caaaggcaaa atttgatcgt acaaaaacgc
    47641 atgttaatgt tggtacaata ggacatattg accatggtaa aaccacatta acttctgcaa
    47701 ttacaaaggt acttgcagct gcttatggtg ggagtaataa accagtagaa tttgatcaga
    47761 ttgacaatgc tccggaagaa aaggcgcgag gtataacgat tgcaacctca catcaggaat
    47821 atgaaacacc taaccggcat tatgcacatg ttgactgccc tggccatgct gactatatca
    47881 aaaacatgat taccggtgct gctcagatgg atgctgctat tctggttgtt gcagcaactg
    47941 atggcccgat gcctcagaca aaagaacatg ttcttttggc ccgccaggtt aacgttcctt
    48001 acattattgt tttccttaat aaagttgaca tgcttgatcc ggctgacagg gatgagcttg
    48061 ttgaactggt tgagatggaa atcagagacc ttctcaataa atatgagttt cctggtgata
    48121 caattccaat tattcccggt tctgcactga aagctatgca gtgtggctgc gggaagaagg
    48181 aatgtgaagc atgcggacca attataaaat tggctgaaac acttgataca tatgtaccag
    48241 agccacaacg tgacagggat aaaccattcc ttatgcctat tgaagacgta ttttcaatta
    48301 ccggtcgtgg taccgttgtt accggcagga tcgaacgcgg tattataagg acaggtgatg
    48361 aagttgagat agttggtttt ggcgaaacca aaaagaccgt cgttaccggt gttgaaatgt
    48421 tcagaaagat ccttgatgaa ggtattgcag gtgacaacgt tggatgtctc ctccgcggta
    48481 caggcaagga tgaagttgag cgaggacagg tattggccaa gccaggttca atcactcctc
    48541 ataagaaatt tgaagctgaa gtttacgtat tgtcaaaaga agaaggtggc cgccatactc
    48601 cattcttcag taactatcgc ccacagttct attttagaac cactgacgtt accggcatta
    48661 tacagttgcc tgaaggtgta gaaatggtta tgccgggtga caacatcaag atggtggtag
    48721 agcttattac ccctgttgca atggaagaaa cccttcgatt tgcaatacgt gaaggtggaa
    48781 ggactgttgg ggctggtgta gttgctaaaa tattagaata aataatttat tgatttataa
    48841 atagcaatgt taatagttgt caggctggtg tacaatcaac ctgacaacta tttacgatat
    48901 aactggaaaa ttatccattt aagaagattg ttatatgcga tcttgttgta tgtaaatgat
    48961 ataatttgat atttggatga aagaaaggtg ttacagtggc aaagcttacg ggacaacgaa
    49021 ttagagtgaa attgagatca tttgatccat atttattaga tcaatcggca gctaaaatag
    49081 ttgcgacaac taacaggagt ggtgcaaaag tagcaggtcc tataccgcta cctacacgaa
    49141 ttgaaaaatt ttgtgtattg cgttcccctc atgttaacaa gaagtcgcgt gagcagtttg
    49201 agatacggac tcataagcgt ctgatcgata tcatcgatcc taactccgat acagttgaag
    49261 cgcttatgaa gttggaattg cctgctggtg tttctgtaga tataaaatca taattatttc
    49321 atctttcatg gaaatttgtt agatagctct ttaaaaaaga gtgtgacagt cttataatcg
    49381 atgcatggga taggaaaatg aaaaaggctt taattggcaa aaagataggg atgagtcaga
    49441 tgtttttaga cgatgggacg gtggttccgg ttactgtatg tgaacttggt ccctgcgttg
    49501 ttgtacagaa gaagaccgtt gaaaaggacg gatataatgc attgaagctg ggatacggtg
    49561 aaacaaaagc tcatcgtctt acaaaaccta tattagagga tttaaagaag aaaaatcttc
    49621 ctttgttaaa ggtattcagg gaagttgaaa attttgatga aaatcttcag gaaggaagtg
    49681 ttattacctg tgagatattt actgaaaacc agatggttga tgttaccggt atttccaaag
    49741 gaaagggatt tgctggagtt attaaaaggc atggattcca tggcgggaga gaaactcatg
    49801 gatcaaactt ccacagagct cctggctcca ttggtgcaaa cacattccct gctgaagttt
    49861 ggaaggggaa aaaattgcca ggaaggcatg gtggaaaaac tgttaccgtt aaaaatttga
    49921 agatagttaa gataatcaaa gataagaatt tagtattaat atctggtgct gtccctggta
    49981 ccgttaattc aattattaca gtacaaagca aataggcagg gttattatgg ttgttgatgt
    50041 ttattcagtt gatggaaaag ttaaaggaca gattgagttg aatgaaacca tctttaatgc
    50101 aaagattaat gatgttctga tatatgaatt gattaaggct gcaaatgcca atttgcgtca
    50161 gggaactcat gatacccggg agcgtagtga tgtttcgggt ggtggagcaa agccatggag
    50221 gcagaagggt acaggtagag ctcgccaggg atctatccgt gctccacagt ggaagggagg
    50281 tggcgttgta tttggtccac atccgcgtga ctatcgcatt gaattaccta aaggcatcag
    50341 gcaggaagca tatcgttcgt tattatccct gaagttcaaa gaaggagcag ttaaagttgt
    50401 tgaggacttt tcagtagaaa gtggcaaaac taaggatatg gcttcaatag ccaaaaagct
    50461 taatgttaaa aaaggtgtac tgatcacgga tagtgaagat accatgctga agagagcaat
    50521 acgaaatatt ccatggtttg tgtataataa tgtaaaacgg ttatcaagca gggatatatt
    50581 ctatactcag acagtgttaa taaccgagag tgctgtaaaa attattaatt cgaagtatgc
    50641 aaaaggtgag taattatgga tgttaatgat atcatcatac gccctgttat tacagaaaag
    50701 gcaactgaac ttgcaaaaca aaacaaatac gtgtttagag tacacaagaa agcaaataaa
    50761 gatatgatag agaaggctat aagcagtatt tttggtgtta caccagtaaa agtaaatgtt
    50821 atgattgtac ggggcaaacg taaaagagtg cggtataact atggatatac agcatcatgg
    50881 aaaaaagcaa tagttacgtt aaaagaaggc gataaaatcg aagtttttga atcaaaataa
    50941 gaatttttta ctatagcgat aaaggtaacg acaatgggtt tgaaaaagta tcggccatta
    51001 acaccaacat tacgatatca gacagtactg gatttcagtg agattaccac tgatgaaccg
    51061 ttgaagtcgc ttacaaaggg caagaaaagt tatgcgggta gaggctttaa aggtcaggtt
    51121 tctgtcagaa gaaaaggtgg tggacataag cgccgctata gaattataga tttcaagcgc
    51181 aataaatata atatagaagg caaggttgct tcattagaat atgatccaaa ccgaagtgca
    51241 aatattgctt taatcgttta taaagacggt gaaaaaaggt acattattgc acctgattcg
    51301 gtaaaggttg gtgatacgat agtttctggc gaaaatgtcg aggttaaacc aggcaatgca
    51361 atgcctttaa aaaacatacc ggatgggacc aatatttata atattgagtt gcataaaggt
    51421 aaaggtggac agttagtgcg ttcagcaggt acgagtgccg ttataaccgc aaaagaaggt
    51481 gattattgcc tgattaaatt accctccggc gagatcagaa agatacattg tgaatgctat
    51541 gcaacaattg gtgaagtatg taataaagat catattaacg ttgatattgg caaggctgga
    51601 agaagtagat ggttaggtag aaggccaaag gtaagaggtg ttgcaatgaa cccggttgac
    51661 catccgcttg gtggtggcga aggtaaatcg tccggaggaa ggcacccctg ttcgccaact
    51721 ggcaaaccta ccaaagggta taagaccagg aagaagaaaa aatacagtga caaaatgatt
    51781 attaaacgaa ggaagtaatt gctatggctc gttcattgaa aaaaggaccg tatgttgata
    51841 taaagttatt taagaaaatt gaggaaatga actcaacaaa cactaaaaaa gccattaaaa
    51901 catggtcacg aaggtcaacg atattccctg agatgattgg gcatacaatt atggtccaca
    51961 atgggaagaa ttttattccg gtatatgtaa ccgagaatat ggttggacac aaattgggtg
    52021 agtttgcacc cacccgaact ttcagaggtc atgcaggcaa agatgataag gtagcaaaga
    52081 ggggcaagta aatggaaaca agggcattat cacgatataa caggatttct gctagtaagg
    52141 cacgccttgt tgcaaatgaa atacggggtt tttcttttcc tgaagcaatt gatattttaa
    52201 aagcaatacc ccgtaaagca tcaaaggtta tcatgaaaac gctttattca gcagcggcaa
    52261 atgctaaata tagcaatcct gaagtacggg aaaataattt atatatcaag aaagttgttg
    52321 tggatgaagg acccacaatg aaacggttca gacctcgtgc acgtgggcgg gcttcacgta
    52381 tacgtaaaag aaccagtaca attgaaataa ttttatctga tgaaaattaa tcagtaaaag
    52441 gtgtaagaat gggacagaaa gttaatccaa caggactgag agtaggaata atacgaacat
    52501 gggattcggt atggtttgct gaaaagaatg agtatcgtaa aaatctccat gaagacattc
    52561 agatacgaaa gtatataatg aataaattta aaactgcagg aattgcaaag gttggcattg
    52621 aacgattccc tgacagaatt aatgtttata ttcatactgc aaggccggga ttacttattg
    52681 gccgaaaggg tgttgatata gaaaatttga aagaagaatt acagaagaaa gttcagaaaa
    52741 atgtaaatat atatgttcag gaagtgaaaa aacctgaacg tgtagcaaag cttgtagcag
    52801 aacagattgc tgcgcagatt gaaaatcgtt tcccataccg gagagccatt aagcaagcta
    52861 taactgcagc aatgcgaagc ggtgcactgg gtattaaggt taatatttca ggacgcttga
    52921 atggagctga aatggccagg gaagaagctt ataaagaagg cagaatccct ctgcatacat
    52981 tacgagcaga tattgattat ggatttgctg aagctctcac cacatttggg cttatagggg
    53041 ttaaggtgtg gatttataat ggcgatgttc tatcaaatgc tgaggaacag gaagaagaga
    53101 aatttgcatt acgaaaaaaa tcacaataat tgaataaagg tgaacaacaa tgttaatgcc
    53161 caacagaaca aggtatagaa aagttcatcg tggacgaatg aagggaaagg cagttcgtgg
    53221 ttctactatc cattttggcg agtatgcgtt aaaatcaatt gaatgtggtg agatcaattc
    53281 tcgtcagatt gaagcggcac gtattgctat cagccgtaag gttaagcgtg gtggcaagct
    53341 gtggattaga atttttcctg acaggccttt taccaaaaaa ccagcagaaa cacgaatggg
    53401 taaaggaaaa ggtaaccctg aaggatttgt tgcacgtatt aagccaggaa gaatattgtt
    53461 tgaactggcc ggtattgacg atgcagttgc tcgtgaagcg ttgtcactgg cagctcataa
    53521 attaccagtt aaaaccaaga taatatcaat acatgataat aattagttct ggagttgttc
    53581 aatgaagggt aaattagatg aattgacaat agatgaattg cagagaacgt tacaggaagc
    53641 aaaggaagag ttacggaaag agcgattcaa aaggacagtg agcagggttg aaaatcccaa
    53701 aaaagcactg cagcttaaaa agcagattgc aaggatttta acattgctca gagagtatga
    53761 gttgggcaaa aggactttac gaagtaaata attttggtgg tagatagtta tggaaggcaa
    53821 aaaccgaaaa cagatcgttg gtaaagtgat aagcaataaa atggataaga cagtagttgt
    53881 tgagattgaa aggatgatga tgcatcctaa atatcataag tttgtacgta aaaccaaaaa
    53941 agtaaaaagc catgatgaac gcaatgagtg ttcaattgga gatattgttc ttatagaaga
    54001 aacaagacct ttgtctaagg aaaagcgtta tcgtttagtg aagatagttg aaaaggtaaa
    54061 atagggttag agctatgatt caggttcaaa caatgctaga agttgctgat aacagtggtg
    54121 taaaaagagt gcagtgtata aagatattag gtgggaccaa gaggcgatac gcttctgttg
    54181 gtgatgtcat tattgttgct gtaaaagatg cacatccatc atatggattg aaggattcaa
    54241 ccgggaagaa agttcatggc aaggcggtct tacgagcagt ggtagtgcgg acaactaaac
    54301 ctgtacggcg acctgatggt tcatatatac ggtttgatga taatgcagtt gcaattatcg
    54361 atactaaagg tgaacccaga ggttcccgta tatttggtcc ggtagctcgt gaattgcgtg
    54421 acagaaattt tttgaagata gtttcgttag ctccagaggt tttataaaaa ggtgttacta
    54481 tgatacgaac tcgattaaaa aaagatgatc ttgtaatggt gactacaggc gctgataaag
    54541 gcaaacgtgg taaagtgttg tttgttgata caaagcgcgg tagggtaatt atagaaggtg
    54601 ttaatttgcg aaagaaatat gtgaggcctt cacaggaaaa ccccaaaggt gggcagatag
    54661 aattgccata cccaattaat atttcaaatg taatggtatt ttgcgaaaaa tgcaaaaaag
    54721 ctgtaagggt tggcatcaag atagatggtg atgcaaaata tcgcgtatgt aaaaaatgtg
    54781 gtaaacgaat tgattagtga gggaaataat ggcagcacga ttgcgtatac tatatgaaaa
    54841 ggaaataaag aagcggctga tggataaatt taaatattca tcagttatgc agattcctaa
    54901 aattgagaaa attgttttga atgttggtga aggtgaagct cattctaatc ctaacctgct
    54961 taaatcaatt attgacgaac tgtcgcttat taccggtcag agagcggtta aaactgtagc
    55021 gcggaaatca attgcagcgt ttaaaatccg tgaggggtat gatgttggtg ctcgtgttac
    55081 gttgcgtggt gacaagatgt atgagttttt agacaggctt gtgaatgttg ctctgccacg
    55141 agtacgtgac ttcaggggac tatcgcccaa ttcatttgat aatgcaggta attataattt
    55201 ttcagtaaaa gagcagataa ttttcccgga gataaacctg gataaagttg agaaaatcta
    55261 tgggattaat ataacaatta aaacaacagc aaaaaatgcc agtgaggcac gtgcgttgct
    55321 tgaagaattc agatttccat tccaagaagc gaggtaagtc aggtggctag cgaaagaatg
    55381 tttgccaagg cccgaagaga gcctaagttt aagattcgtc agagaaatag atgtaaagtt
    55441 tgtggcaggc caagaggcta ctacaggcgg tttgaacttt gcagggtttg tttgcggaag
    55501 ttggccggtg aaggcctaat cccgggtgtt aaaaaatcat cctggtaaaa tttaataggg
    55561 agtataagcg ttatgagttg tatttctgat ccaattgcag atatgttgac ccgaatgcga
    55621 aatgcaatta atgcaggaca cgttaaagtt gatataccct catcaaaaat gaaaatggca
    55681 attgcaaaga ttttaaaaga tgaaggtttt ataaaaaact ataaacttat tgatgataat
    55741 aagcagaaaa cactgcgaat ttaccttaaa tatagtgcag ataatcagtc agcaattatc
    55801 aagatgaaac gaataagctc tccaggaaga agagtatata ttaaggccga ggatcttaag
    55861 ccggtatata acaatatggg tattatgatt ctttctacat caaagggtat tattactaat
    55921 aaggccgcca aacagttgaa tattggcgga gaagctttat gtgagattct ataaggggca
    55981 gccggatatg tctagaatag ggaaaaaacc gatagtgata ccaaatggtg tatcagttga
    56041 tctgagtggt gaaatgatta ctgttaaagg tcctttagga accttgcaat ataagttgca
    56101 ggatgctatt aatgtagtta ttgataacaa tgttattaat atagcaaaaa aggatgaaca
    56161 gtccaaagac aagaaagtca atgcattatg gggattaaca agggcattga tacataatat
    56221 ggttgttggc gtttcaagtg gatttgaaaa aaatttggag atagttggtg ttggatacag
    56281 ggcaacccag aaaagcaacg atgttgagtt tcagcttggg tattcgcacc cagtactgtt
    56341 ttcaccgcct gctggaataa aggttgaagt cgttgagcca acaaaattaa aagttaaagg
    56401 cattgataaa cagttagttg gccaggtggc agcaaatatt cgcaaattac gcccaccaga
    56461 accatataaa ggcaagggta tccgctataa ggatgaacag atacgaagaa aagctggcaa
    56521 gaccggtaag taagactatt tgggtgtaaa ggatagtaat tatgaataga ttgaaattga
    56581 aaaaagtaag attcatgcgc agaagaatgc gtgtaaaatc caaaatcaga gccaatactc
    56641 agcggccgcg tatgtgtatt tcacgaagca ataaaaattt ctatgtgcag attattgatg
    56701 acagtaaggg tcatacgctg gtgagtgctt caactcttga gaaggatttt ccaataacca
    56761 atcaccgaag taataaagca gcagccaagg aattaggcaa aatcattgcc cagagagctg
    56821 tggcaaaaaa tataaaacag gtagtctttg acagaaacgg ttacctttat catggaaaga
    56881 taaaggaatt tgctgatgca gcccgcgaaa atggattaga attttagggg agatggtaat
    56941 gaagattttg aaaaaacgtg taaagcctga aggactggag ttatccgaaa agataattat
    57001 catcaaccga gttgctaagg ttgtaaaagg tggtagaagg ttttctttca atgccatgtc
    57061 tgttgttggt gataataaag gacatgttgg tataggattt gggaaagcca atgaagtgcc
    57121 agatgctatt cgtaaaagtg ttgaggatgc caaaaagaat ttatataaaa taaatgttaa
    57181 taaaaacact ataccccatg aggttatagg aagatttaaa tcaacgcgcg ttatcttaaa
    57241 gcctgctgct cctggtacag gacttattgc cggtgattca gttagagcag ttgtggaaaa
    57301 ggcaggcatt gtggatatat tatcaaagac cctggggtca cgaaacccgg ttaatgttac
    57361 caaagcaacg ataaatggct tattgcaatt acgaagcctg aaggacgtag ctgatttacg
    57421 tgaagtaccg ttaccaaagt tatggcaacc ataagaggtg tatatggcta agaaattacg
    57481 tgtaaaacag atacgaagtt atataagtgc aaaaccaaag caaaaagcaa ccctgagggc
    57541 tttaggattg cggaaaataa atgcagaaag agtacatgat gatactcctg taatcagggg
    57601 aatgcttcaa gttgtaaaac atatggttga agtttcagag attaacgaat aaaagtagta
    57661 ggtgtgagtg ttatgcaata tacattaaaa aaacctgaat atataaaaaa gaaaaagcgc
    57721 attgggtgtg gccctggaag tggtcatgga aaaacaagct gtaaggggca taaagggcaa
    57781 aaagccaggt ccggtgctca ttttaaaccg ggttttgaag gtgggcagat gcccttgcag
    57841 agaagggttc caaaacgtgg atttaacaat gcacaattta aggaagaagt gcaaattata
    57901 aatatcaaag atatagcacg attagggatt caggaagtaa ctcctgaagt tttgtaccag
    57961 aagggtctga tcaaatcaca gttgttgccc gtgaaactgc ttgctaatgg tgatatcgct
    58021 tcagcagtta aagtaacagt tgatgcagtt tcatcttctg caaaagagaa aattgaaaag
    58081 gccggtggaa gtgtttcgct tcgatcataa ttatataaag aggtcataaa gaaataatgc
    58141 caaatgtaat tacaaatata ttcaaaattc cagaactgcg gaaaagggtg ttatttaccc
    58201 tttttatttt ggtagtatat cgtattggtg cacatatacc aataccaggg gtgaacattg
    58261 aggcgttaag ttcatatttt gagatggctg caaaaagcca tggtggaggg tttgttgatt
    58321 tttttgacct ttttgcgggt ggcgcgctga aacgctttac tatatttgcc ctggggatta
    58381 tgccgtatat ttctgcatca attataatgc agcttttaac agttgttatt ccggccatag
    58441 agaagttatc taaagaagga cctgaaggat ataagaagat aaaccagtat acccgttatg
    58501 ggactgtgct tttgtgtgcg ctgcagggct ttggcctcac gatgtggatg aagtccatga
    58561 catcccccaa gggtacagtt gtgcctcagg atgccggcat tggcttcatt atagttgcag
    58621 tacttgctat tactaccgga accatgatac ttatgtggct tggcgaaatg ataacagaac
    58681 gaggcattgg taatggtata tcgctaatca tttatgcagg tattgttgtt cgtgctcctt
    58741 cagcgtttct tgataccatg aacaggataa tcagcagaag tgaccctatt attgcgctca
    58801 tactgattgc attatttatt ggggtagtgg ccgcatcaat actgttaacg ctgggtgtac
    58861 ggaagattcc agtacagtat ggcaaacgtg ttgtgggaag gcgcatggta cagatggcat
    58921 cgcaggctat acctctgcga gtaaatgcgg caggcgttat cccaatcata tttgcttctt
    58981 cagttattct ttttccagca cagataagta atatgctggg tgcaacaaaa tacgctattt
    59041 ttaccaagat gcagtactgg ttatcaccag ggcataccgc atatatgatt atatacagct
    59101 tgttaattat ctttttctgc tatttttata cggcaataca gtttaaccca aatgatctgg
    59161 ccgaaaattt gaaaaaatac ggtggattta taccgggtat acgaccaggg cagaacactg
    59221 ctgattatat tatgcatatc ttaaacagga ttacgttacc aggttccatc tttttggcac
    59281 tcattgcgat agttcctgat tttattatac gcatatggcc tgaagaggta tcaccgggaa
    59341 tggcatacct ttttggtggc acatcattat tgattatcgt tggtgtggcg ctggatacat
    59401 taaagcagat agagtcacag cttttaatga gacattatga tggcttcttt aaaaaagggc
    59461 gtataaaagg tagaagatat taaatttaaa agctatattt attttttata caattatatt
    59521 ttgaggcgtt ttggctattt ttatttacag tgaagaggag atcaagcggg ttgaggatgc
    59581 atgcagaata actgctgctg ttctggatga gattgggaag catgttgatg caggtatcac
    59641 tacttttgag ctggacagga ttgcaaggga tttaataaaa aagtttaagg caatcccggc
    59701 atttttaggg tataaagggt atcctgcagc tatctgtaca tcaattaatg aggttgttgt
    59761 ccacgggata cccagtaaag ggcaaatctt aaaaaaaggc gatatagttg gtattgatat
    59821 aggcgttcag tataatggat attttggtga tgcagcaaaa acatttgttg ttgggaaagt
    59881 aagtaataac gttaaaaaat tgcttgaagt tacaagggaa gcacttgaac gtggtatttt
    59941 acagtgtaaa gatggcaatc atctgactga tatatcgcat gctattgaat cacatgtagt
    60001 tcaatatggt tttcagcctg ttcatgagtt tgttggccat ggtattggaa ggaatctgca
    60061 tgaagaacct tcaataccca actttggcaa acctggcaaa ggtcctgtga taaaaacagg
    60121 gatgatcttt gcaatagaac caatgattaa tgaaggcaca tatcaggttg agattttgca
    60181 ggatggatgg acagcagtaa caaaggacag aaaattttca gcacattttg aacatactgt
    60241 tgctgttgtt aatgggaatc caaaaatatt aacacacaat taattttata gtataaggag
    60301 tttaaaactg gatggccaaa gaagaaccga ttgaagttga aggtgaagtg gttgaagcgc
    60361 ttcccaatgc catgttcagg gttaaattag ataatggtca tgtagtatta gctcacatat
    60421 ctggcaaaat gaggatgcac tatatacgaa ttttacccgg tgacagagta acagtagagt
    60481 tgtcgccgta tgatttaagc agaggaagga taacctatcg gtccaaatga tagttaatgt
    60541 ttgaggtata gatatgaaag tcagagtttc ggtaaaaaag atttgcagtg attgtaaagt
    60601 tataaagcgt catggtgttg tacgtgtgat ctgcaaaaat ccacgtcata aacaacgaca
    60661 gaagaaaaga acagcgaaat aatatgaggt agaggtatat atggcacgaa ttgcaggtgt
    60721 tgatttacca aacaataagc gtgttgaagt tgcgcttact tatatttttg gtattggacc
    60781 ttcatcatca aggaaaattt tagaggatgc acaggttaat cctgatactc gcgtaaagga
    60841 cctgactgat gaagagatta atcagttgcg aaaaatcatt gaaagaagtt ataaggttga
    60901 aggtgatttg cgaacccaga tagcaatgga tattaagagg cttatggata ttggttgcta
    60961 caggggtata cgacacaggc gaggattgcc ggtgcgagga cagaatacca agaacaatgc
    61021 acgtacccgc aaagggaaca gaaaagtatc ggttggaagc aaaaagaaga aatagtaagt
    61081 caatgataga ggtgtacagt gaagcagaaa cgaacaaaga agcaggaaaa gaagaatata
    61141 ccagtaggta aagcgttcat tcaagctacg tataataata ctattattac aataacagat
    61201 atgcagggta atgttgtatc atgggcaaca gcaggtggag aaggattccg tggttcgcgg
    61261 aaatccacgc cgtttgcagc acagatggct gcaaaagctg ctgcgcagaa agcaatagat
    61321 aatgcaaata tgcgaacgat tgaggtatat gtaaaaggtc ctggtattgg acgtgaagca
    61381 gctatacgtt ccttatatca ggctggttta aatatctcaa agattaaaga tattacaccc
    61441 ataccacata atgggtgccg accacgcaaa cgaagaagag tataatgtga gggtttagtt
    61501 tatggcaaag tatacagggc ctttatgtaa attatgtaga cgagaaatga ctcccttaat
    61561 gcttaaaggg aggcgttgtt taaccgataa atgtgccatc aagcataaga aatatccccc
    61621 gggacctccc cgaaagagga gaaccaaatt atctgactat ggcgtacaga tgcgtgaaaa
    61681 gcagaagatt aagcgcagct atatgctttt agaaacccag ttcaagcgta cgtttgatga
    61741 agctacacgc cttaaaggtg ttacaggcga gaatatgctc gttctgcttg agcgaagact
    61801 tgataatgtt atttatagat tgggctttgc atcttcacgg ttacaggcac gacagtttgt
    61861 tcagcatggg catgtaattg ttaatggcaa gcgtgttgat atcccttctt atcaggttaa
    61921 ggtaaatgat attgttgaga tcaaggaaga ctttaaaacc aatacaatgc tggaagatgc
    61981 tatcaaattg tcaaaagcag tgggatcagt tcctcaatgg ctggaagttg actatgagaa
    62041 gaggagtggc aaaataatac ggttacctga acggaaagat attgagccga tatttaatga
    62101 acaggtggtt gttgagttat attctaagta agttatgtgg ttaagtatgt gtttatttat
    62161 tggaggtaat aatgtcccat aaaaacctat taaagggttt tcgaagacca tcgaaaattg
    62221 tgtttgaaca tgatgaagtt aatcaaagtt acggaagatt tattgctgaa ccttttgaac
    62281 gaggatatgg tttaactata gggaattctt tacggcgagt tttattatca tcgattgaag
    62341 gtgctgccat tactgcagta aaaatccagg gcgttccgca tgaatttact gctatcgatg
    62401 gtgtagtaga agatgtaacc cgaatcatat taaatttgaa aaaactacga tttaaatacg
    62461 aaagcgaact gccaaaggtt ctgcacatag tcaaggacgg cgaagcagaa ctgacaggag
    62521 cagattttgc aatagatagt gatgtggaaa taatgaatcc tgaaactcat attgcaacta
    62581 ttgagcgtgg tggcaggatt gatatggaag tgcagattga gcgtggcaga ggctatttgc
    62641 ctggtgaaat aagcaagtcg agtgtagaaa ctattggagt tattcctatt gatgcaattt
    62701 tttcaccaat acgaaaagtt aatataaagg ttgaagatac acgagtaggt caacgtactg
    62761 actatgataa attgattata gaaatatgga cagatggttc ggttaagcct gaagatgcac
    62821 tggcgcaggc agcaaaaatc ataaaagatc atatgaccat atttattaat tttgaagaag
    62881 aagttgagga agaggaagtg gctgtagatg aaagccacga taagctgaaa gtaatcctgg
    62941 caaaatcaat tgatgagttg gaattttcgg tgcgtgcata taatacattg aagagcttag
    63001 aattgaatac acttgaagat gttgtacgta aaactgaaga tgaacttaaa aaatcaaaac
    63061 atttcagtga tcaggtacta gctgaaatta aaaagaaatt agaagagcat catttgagct
    63121 taggaataaa ggaataagag gtagtgacat tatgaaacac cgtaatagtg ttagaacatt
    63181 agggcgctcc catgagcata gaagagcaat gtttgccaat atggtgacat cgctgtttca
    63241 gcatgaaaga ataattacca caaagcagaa gggcaaagag ttaaaacgga ttgctgagcg
    63301 attgataaca agggccaagg aaaacttaag ccttaccgaa aatgatagtg cacgtaagct
    63361 ccataacaag cgtgaagtaa tgcgccttat taaaaatgaa gatgttgtta agaagctgtt
    63421 tgatgatatt gcaccacgat tcgtcacacg cccaggtggt tatacacgta tgttccttct
    63481 tgacaggcgt gaaggtgatg ccgcacagat ggcaattgta gaacttgtgg tgaaacctga
    63541 aacaaagaaa gaagataaaa aagaagataa aaaagataaa aagggcaaaa aaaccacagc
    63601 ataagctgtg gttttttatt tgatgtctat tttacttttt tctctaatcg ggctttctca
    63661 attgaatgta ctaaactgat attgtcatta gcatctactt taaattcttc gggtatgcga
    63721 tcgtcataaa tgggatccag cttattgctt tcaaggccat atttacgctg tatggtgtcc
    63781 aattgatgca cttttaccat tttatgaatt tcaattccat attgttttgc aagacgacaa
    63841 aaatcgataa ctgcaagata ttgttttatg ataaattcaa gctcatcgtg cttgatattt
    63901 tttttgcttt ttaaatagat tatatcctga ttattataga tattttttgc ttcctgaagc
    63961 tttttgtagc cattctttaa gtatcgttca attgtttccc tgtcaaaaaa aaggtggtat
    64021 tcttcagggt tatatggttt aatttcttca acagggttat agggtttttg aaaatatttt
    64081 tttaaggtac tgtttttgtc atactttatg ataatgtcaa aggattgctt tgaggttgag
    64141 tccagaatct gctgagccct gttaatatac agggtggcaa gtgcatcggt gagagtaacc
    64201 agttcctgtt ttaccttata atacttctgg tgtgagctgt caaaattttg cccgtaatat
    64261 tcttcagaag cattttgaaa aagcgtttgt atttcctggt atttattttt tgtagtctca
    64321 tcaccaaagt tttcaaccat gatgcgaatc tgccgcagca gtgaaagagt aaggaaatag
    64381 tcattctgtg ctttcacggc agtagaaaca gcaaatgatg gagccgtgat gataagcact
    64441 ataattgtgg aacacagtat aattagatgc ttatgcatat cattaacctt catgtgatag
    64501 tcttttttat tgtatcggta aaatatgaaa actgctttat gtatattttt ataatagtaa
    64561 atgatttgtt tatcagttac tatcgccaaa aattttaagc gataataatt aataaagtaa
    64621 atatttgatt gcaaagatac aggtgatact gttacatgaa aacaataact gtatttttgt
    64681 tgtggggatg aacgtatgga aaatgaggat accaaaaaga agatatcatt caggcagaaa
    64741 aatcccacca tatgtccggt atgtgggtat gaattttatc gcgaggagat gctgacgggt
    64801 ggtggcaggc ttatagcggg caaattaacc gatgaattgc gcagaaccta tgaaaaaaat
    64861 gaaaaatggg gtgtgatata tccgcttgcg tacgtggtaa cggtatgccc acagtgcatg
    64921 tatgctgcat acccaaagga tttttctacc ttacaggctg atgaaatgca gaagataaac
    64981 gcaactacca atgcacgaaa acagtccatt gataagtttt ttggcgggct tgattttaat
    65041 caggataggg gattgtatca tggggctgca tcgtatttgc tggcaatgga ttgctattcg
    65101 ttcaggaata aaaatgtggc gccgacgttt aaaatggctg tcagtgctat acgggcagca
    65161 tggctttttg gggatctggc taagttagag ccggataagc cgtataaaaa gatttcggac
    65221 tttttttaca aaaaggcgta tgattattat tttaaggtgg ttgacatcat tcaaactggt
    65281 gctgaacctg ctgatgcagc aggcaacatg gggccggata ttgataaaaa ttggggttat
    65341 gatggcatac tgtatatgtg tgcaatactt acgttgaagg tgggtgcaaa
//
LOCUS       DAORYL010000007        64007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_387_length_64007_cov_12.822414, whole genome shotgun sequence.
ACCESSION   DAORYL010000007 DAORYL010000000
VERSION     DAORYL010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 64007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 64007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64007
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_387_length_64007_cov_12.822414"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PLV81_02860"
     CDS             <1..759
                     /locus_tag="PLV81_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09074.1"
                     /translation="LYSVRAIKLCNNKINRVNHMKRVIYVICLLFSIQSSADTIDTII
                     NNFAVESIDATIVQVIAETGKPTIRYEGRYRAYKQNMRIDYSKPFPQIVIVKEGNLQW
                     YYPESKELWVMHTMNAPVTHPFAQFFAFKDRIVIKSKENTYYGLFKRAYKYILIDTTT
                     RTRIEVIVDKHKGYPVKKITFTDDNVEIMREQYEDYAYINNVWFPTTVEVVARTRNGI
                     TRNITRYYDVHLNMKIQPDIFILQLPKGVRAKQM"
     gene            767..2143
                     /locus_tag="PLV81_02865"
     CDS             767..2143
                     /locus_tag="PLV81_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09075.1"
                     /translation="MRQLFFLLLFIFYSTIGMAKVVVLGLTVPINIADIENKSVVSEQ
                     QAKVLAIALTNKLHESGYTTSYVNRYQLENDICYLYVVESHIADIQLAADSALKEYIM
                     QDLQKLLGAVYNRKTVHAFLVQLKEKYLLDLIQINVVNYNESDDVSLVIRVRARKIAW
                     SFEVATLPIYGITPSIVVTVPMHKAVMTMQGQVGFNEERVTLEKGVFEYMSFPATIGF
                     HAGVLVSREYTVWERYGTDFTVTIAKPYAGIGIFKNRGVFDISAFLYGVATYYKVSEH
                     NTAELEDTYKDAGIELRIQSKDDRSIAKKNKTLQASLFAGFSQDEYLLTSRALGIAPL
                     QITSRFYVIPSAYSFYTNSFKRIYTEYVFDSYLLGFASRYTSTQSRHIAKLELMNEVI
                     YEFVFFEIFGASGVYKSEYNRWEKTSSWGAGADIYYNTIIINIGCAWNTDKQFSQYYI
                     FTGVRAKL"
     gene            complement(2167..3081)
                     /locus_tag="PLV81_02870"
     CDS             complement(2167..3081)
                     /locus_tag="PLV81_02870"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOM09076.1"
                     /translation="MPKEMMNTKEVAEYLDIHEKQVYVLIKENKIPCTKITGKWIFPK
                     SLVDEWILSQSKINIKGLQPETEYGGHALLAAGSNDPVVDILLNELKEINPLHSIFTC
                     STGSLRGIHLLSKRVTHIAWSHLYDPKTKSYNIPFIQEICTNIPVVIIHLFYRKIGIV
                     LSPGITHILHNIRDIIHPDIVFINRQEGSGIRVLTDYLLKENNINPQKVKGYTNVKYS
                     HIEVGLAIQNGEANAGIASVAIANFFNIPFIPLTRESFDMIVTKEIFFNDAIQSLLKI
                     IKSDKFRKKMSSIAHYDFNDSGDIVYSQ"
     gene            complement(3708..4499)
                     /locus_tag="PLV81_02875"
     CDS             complement(3708..4499)
                     /locus_tag="PLV81_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09077.1"
                     /translation="MKTLWNIVTSINSTFYLLIITSIMLFSGSLYTNEYFKFFSDLDY
                     SNIFHWLMNNMVDKFFLVWWIIGLFITVTFIMINTFFCTVKKLYTLCTKRGLISTKQL
                     IGKYCISIIHIFTITILMAHGISHGFSKTYTFPIEEGKSIKPGNNQNLYIEKILITYY
                     PKRSLFINCIKDIKLTMVECGDVKKRITISPLNPVLYKDYTLFVIQNKKKNKQNAPSP
                     MTNNFNEVKFYLLARKDSGYTLWVLSFTVIIVLMSLYYWLIYKEN"
     gene            complement(4504..5256)
                     /gene="ccsA"
                     /locus_tag="PLV81_02880"
     CDS             complement(4504..5256)
                     /gene="ccsA"
                     /locus_tag="PLV81_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOM09078.1"
                     /translation="MIHSIHSVLFASAILYIISFAAIVTGKEQLSLVTLIIGLITHSV
                     YLLLRIFMSGYPYLQGAFDSVFLTPWTIAVILILLYYRYNNYTIFYATVILIIAVLLS
                     LLYPKGIIPPSPNKSTYLAYIFFMLESLAISLFYISGFYSALYLLQTDSDYFNFSYKC
                     IIYGFLLYSAAQFVGALWCYAGWGIYFKWSPRHLHSALIWVLYINFLHLKYIPDFTNR
                     KKSVYALACSLITFIFYISSYLHEYNIPRIGN"
     gene            complement(5265..6335)
                     /locus_tag="PLV81_02885"
     CDS             complement(5265..6335)
                     /locus_tag="PLV81_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09079.1"
                     /translation="MERLKQYLPYLLLGILALYIVCVHYFRNNPRYVIAITGATPKAI
                     VAPLPEGISLTITGYTARVYTLTSKSLAAFAPAYVFTREVTDTGKYTGAYRYHGIPLL
                     NILEGIKIKKPADAVFDGPHDIIVQFKSRSGKVSKCSYGELVMSGNMFNYILAYKREP
                     IQPAKEGNTYSKNLYREDLKGLRLVIACDRDTSRYLDDIVEINLTVPQYDCSSLPKTE
                     KGKQCSSSYILCISPDKSHRATFTGVSQQTISDWVQVGHGRGYKGIVQVKGFNLKSFL
                     YQNFSYIPHDAWFMFIGCDGYRCLFSYKEIFEQDAGNKLMIVTHINGKSAQGNFMVAS
                     TGDFFVDRCVWGLSHIVLLPGE"
     gene            complement(6449..8887)
                     /locus_tag="PLV81_02890"
     CDS             complement(6449..8887)
                     /locus_tag="PLV81_02890"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOM09080.1"
                     /translation="MEKIVIRLLALYVIVLSTGISFAQNTTETDNQQPKEYEEKIDTQ
                     STQPRYKVFHPGEIVISETKISNIEKATTNTQITEKDIEARNDKALSDSLQMVPGVLI
                     EQTAKGFTGLSVRGLDHQYVAILIDGVPVIDPYYGGKNIDISMIPVGNVQRIIVNRGI
                     SSALYGSLGSAGSINIITKKPEELYAKAKAEYGEYSNYFISAEAGLPVGNFYTWIAVS
                     RQYSEGYEISEKLTRNKRQEWFDKIVSYDLADSNSGTAVTIVHPLFSDIDLDAVDNYL
                     NDTGKWNNTEYTKHTIAGKIGYSLSDATEIGIAANYYRNEMYSNTFQNNSLHYFDEEN
                     NVWSLPVYTRDYVDSSGRSWRDIFSNRAFYWPEDWRFNVSPYLTTRFGNLKLRINTFY
                     VKQRNNLEGYFNQDYTIAQMFPNSTNYNGNAQSIFEETSYGAYILPQYALSKNNTLYA
                     SIHFRNEQHDKFEKALDETSQLATTLGTDEYKVADMRAQYISIAVEDEWDVHTGAGIV
                     FLSFGASYDAQKLSTLRVYDKTTDALIHYPEMDEDSYLWGTYDSCNPVASAMYEAVPN
                     FLTLRFAGGIKTMFPNLSQYKDIGQDILDNNEEYQIKPETIYSSNTGFEITVLQNTLS
                     FRNDYFYTKVKNKIARIYDPDSAYEKYKNLDGMTAQGIESTIKYKASLYTIDIECTLN
                     YVYTYARNDAVTSLTYGKRVEGVATHQFLAQLHAAFPTQTECIFWATYTADQIVYVAN
                     TSFDFTSTSFSADAYDTVQLHNPLILNIKLQQKFLHHYLLYVTCKNILDDYAADPFNP
                     GPGRMFYIGLNAEL"
     gene            9154..9582
                     /locus_tag="PLV81_02895"
     CDS             9154..9582
                     /locus_tag="PLV81_02895"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOM09081.1"
                     /translation="METECGIVKRVDGNYAVIEADAVSFCSTCENHTCTMRQSRGRQI
                     MIENTLGAATGDRVFFVFPSKGILLSSVVLYGIPIVFLIAGIIIGAVLSWPFHDKDFS
                     GIITGMVFLIFSFGIMRLASQYIVQHRQLTPTMVKVEKPK"
     gene            10120..10608
                     /gene="greA"
                     /locus_tag="PLV81_02900"
     CDS             10120..10608
                     /gene="greA"
                     /locus_tag="PLV81_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOM09082.1"
                     /translation="MQVNIEDKKKQIKEEIEKLQYEMKIELPQRIAEARAKGDLKENA
                     EYHAARERQSFVQARIAYLSKQLSLMDSIDISSIEQGKVEFGSLVTVRNLDTGEVGEF
                     TIVTPDEVDASAGKISLSSPIGRALHKKTVGDEVTVQIPAGTRRFRIEKLITIHGEEL
                     SL"
     gene            10624..11847
                     /locus_tag="PLV81_02905"
     CDS             10624..11847
                     /locus_tag="PLV81_02905"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOM09083.1"
                     /translation="MAIIKEFTALCPNQELVSKVAELPYDVVTTEEARSLAQGNPYSF
                     FHITRPEVDLPANNNPYTNEVYRKGKENLQQFINSGVLLYDDAAYLYLYTLTMQNHQQ
                     TGIVAGVSIDDYINGIVKKHELTREDKEIDRMNHINIVGAQTGLVYLFYKNNDALQEL
                     MKESILSPVLYDFVAADGVQHTVRKIKDNLLAAKIKDALQPLVLYIADGHHRAASAVR
                     VGLERRKSGFSGGEAFNYFVATIFPHSELQILPYNRVVKDLNNHDLRSFMNELSQSFL
                     VEKSDSGIVAKPHEIRMYCDGQWYVLHYKKELPLNDVERLDVAILQNVILAPILGIQD
                     PRRDSRIDFIGGVNAVNDLIALVNSGKFKIGFSLYPTSINELIAISDKGGIMPPKSTW
                     FEPKLRDGLLVHVIG"
     gene            complement(11854..11926)
                     /locus_tag="PLV81_02910"
     tRNA            complement(11854..11926)
                     /locus_tag="PLV81_02910"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11891..11893),aa:Ala,seq:ggc)
     gene            12112..12432
                     /locus_tag="PLV81_02915"
     CDS             12112..12432
                     /locus_tag="PLV81_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09084.1"
                     /translation="MGIEIEKLKKENLSLTEEIAELRKIIQTYENVLKLNEQELSNAY
                     QIIAMYENIVEYSRSELKLATETAQARENASQLSRLELMQAFEKIKQLEEENKRLRAQ
                     KGNA"
     gene            12473..13825
                     /locus_tag="PLV81_02920"
     CDS             12473..13825
                     /locus_tag="PLV81_02920"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM09085.1"
                     /translation="MLVTYAIIFLFIIILVIFILYFANIYIFPRKLEEIQQLIESGQT
                     KIAIRKLNDIIEKDDRNHYAHYLLALAYEKENNIQYAILEYRQVLKISKFDDKVDEIV
                     IRKRLASIYKARNAIEEAKKEYLLLTKLDPANYEPFYELGVIFFNAGQLEKAAPYFKK
                     SISNNSRHAPSYYYLGQVQYRMQNYADAKQSFIEAIKIDPNDYKSHYFLGLVLRQLGD
                     YEWAIKEFETAQKSDEIKVKCFLAKGTCFLEREQLPKAVVEFERGLKFAKRGSDTELN
                     LRYFLADAQEKMRDLHSAISNWEKIVAVNPTFRDVQEKLKAYAEFRQDDRIKDFMIAG
                     LAQFEHTCRKMVEAMGLTVMDVDIISDTEIEIIATDTEGKWRNTRRTNKIVRILRTTD
                     VVNDKLLRKLHEKLKEKNATRVIIITAGEFTQSAIDFSNTRPIELYGKSDLLRLLKKT
                     "
     gene            13830..15644
                     /locus_tag="PLV81_02925"
     CDS             13830..15644
                     /locus_tag="PLV81_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09086.1"
                     /translation="MINKDILILRQTPFFKHLMDEELSALLKVVKPLAIPKGGIINCD
                     SHSLYIVKKGVFEIQKHAESIYITPPSFFGELPFCEKGSGIVRAISDSIAYELKDTDL
                     YQFMGNNFKALRGYLRIADFYRFSLTDAGKKLNRNKTQIITVFSTEKHCGKTTVAVAA
                     ATLSSFNKKTILLDASYDGISVFDMCGKQLLPPLSQKSPDEVTGQKFIEQRIEKVSES
                     LDILNVAFGSKVRLNPDIINPLLFYLSFEYSYIVIDCSNSDVALRDTILHNTDILIEI
                     INNKSISKLQAINDSLLTQGQQVIYIVNKHFEKTSKIDDSHKDFETISIEFGETVFQA
                     ILRTKSQHDILFHEILSDKKALVFASGMYETLYYAGIFETLRDFDNIPDIIYTAGIPY
                     VVLIAFLHSSSLKEYKKLLLSYFSDDKLHSMLDVTFPDKYLFKNDPLKSFSSSVCNAQ
                     RLEYYNIIPYTILFSNNSPRMFTTGDIRDVVAASFAFLPLFEGVSINKEEYHSSFPLY
                     PAMPETLLRTHIQQISFISNNTMFNPLSLKSKVLGIFRKFVDFLPYYYLYACDTGAVC
                     NKEFSLQIPEVTSIDDLIEKTFDNKENFDDIMIKIVGG"
     gene            15645..16091
                     /locus_tag="PLV81_02930"
     CDS             15645..16091
                     /locus_tag="PLV81_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09087.1"
                     /translation="MRSKATNILQFGVLLTGIIYVTIGLLYGFSPILFANIFGIEVNP
                     DWYNLIKYDTFTSPLYHFSRVFALLMAVAGLSMILPLFDPLKYRGMIYYNGILFPVVA
                     APVLLVNGLTYDHLILTICGVLLLVLFFFVGFGLMITRRQAKMGQE"
     gene            16275..16613
                     /locus_tag="PLV81_02935"
     CDS             16275..16613
                     /locus_tag="PLV81_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOM09088.1"
                     /translation="MNLQTKKVGNVVVVYLQGRLDVHLSADIEKEINKLIKDEPNAHL
                     LLNLSGVEYMSSSGLRIFVSTMRILKESNRKLKLCNMNSAVKKIFEVVELMDMFEIYD
                     TEEEAIASFK"
     gene            16733..18883
                     /locus_tag="PLV81_02940"
     CDS             16733..18883
                     /locus_tag="PLV81_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09089.1"
                     /translation="MKNFIIYQKYNYFVWPYWISRYVNTVNERIQSLPFFLYNNHCRT
                     WYTINSFLSSGSIRIDPSGMVHPVWSSWSLEFWIYTDNSLIQFHNDNYIQQGIDNGVV
                     KTSYIHPDFTFKQSVYITHTNIDEAVIVNNVHYKKKNKAYFIIAVRPYTTERLSGLES
                     VELVKNNFVRINNEYHCALLMKPDYVLTGNEISGDIDIRRKENKHKVTSNARMATMAF
                     VYDISNKESEIAIRVAVNNESKISVPKIIIKSTQNEFTSYIDLRMKEGVGITVGKNSI
                     NEWVNFCKFSALVEAANIDISDTNQNEDIARHYYTIMTLNRMGHFQQSLSIINHILKN
                     IKIKDDFESILSAAYVCNMLGDYYRIVKDTSFLQNNYPVCKQYAQNILQFSKELKNIK
                     KYILLDHSDENAIHQIWNIPVIISALENLAFMSRSLGLFGDETKFLAEANKLEASVVN
                     VLFNVSQETSKEEEKLIEWLPKHGKPLRKQKISVKESLEFANTINTSNNRILLYTSFL
                     YPHIVHIIRKYTVQRIIDDIINAGKGLPLYNTIISGIDVISSLNLANSMLYAHDKRAI
                     DLIYAIITLCKKRKFMPDFLDPVSGHAIVKEPISIVAVSLLFQAIRNICFIDHPERLE
                     LFPVPVEDWFGDSQIVITNAPSRFGNISFVTTSTSSEFVIQFTDVPRFVPHDVLINLP
                     FKAKIKKADDFIVKYETEKSWCINGWPSEIRFKR"
     gene            18946..20490
                     /locus_tag="PLV81_02945"
     CDS             18946..20490
                     /locus_tag="PLV81_02945"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM09090.1"
                     /translation="MITYILVSLFIISAFLYYLVYIRPKFSPLYKAQQLVEQNQLLDA
                     ILEYKKVIDEYPDDFVVHYRLADLYLAVGEIDQAVIHFEKVLEIGKFNYEVDQLSVQK
                     KLAKSYYMRDEIEKAFQTYMDILKNYPADIESLYHIAFICLGQEEFDIAQRYFDRLVK
                     LKNDNYEILFGAGICYYQNMRIQDAIDNFKLAVQIKPQSEIAQIGAAFACNRKRDYRQ
                     ALGYISRIADTAQDPDVLYLAKRFQAFMLMRLKKHDEGIKLFQDLLDIARKNANDDAE
                     LLALYDLGYAYLKNEQTSLAYEAWNELYRRDRNYKNIQYLVTQLRREMEVDKFSKEES
                     VSVTETITTWMDDAFPQNLLWEMCGLKSTKKYNLKDIVVTTKISYDDGKTVSVSDDVM
                     DRYVALDTENFRIISNRLVSKMGFKVDQILQTYREADGVDFLAINPETKDKVLVWVRR
                     WDKTNVGEITLRNFAQAINDMKVQQGIFITTANLTQAAKESLKKLPKVTVIYPEEITK
                     LLRGLI"
     gene            20490..21419
                     /locus_tag="PLV81_02950"
     CDS             20490..21419
                     /locus_tag="PLV81_02950"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOM09091.1"
                     /translation="MICPESNVVSIPLDAIQCDNNYFKISKPLSRELVRSVLHYGIIE
                     PVLLIKWQEQYVPVCGHNRIIAAQQAGIKDVPAKIVTELSMDDFLQIVRVKLYNNALG
                     TIGKIKSLYLLYDYFNADKKKVMAIAQELSLPASLPEFKNIRSIIELPPVIIQYIDSR
                     SISPKFVMPILRLPQSSIALLEKITSACQLRVNYFNSMVVMIEDIYRMGKDKLLGDSI
                     EFLLTKETILDDDIHNCIYSIRYPQYTEYMVRINEIKKKLNACGIKVDIPAYLEGDSI
                     NVIFEVKKNKPLHPLLKKDQDILGLIELLIGLL"
     gene            complement(21453..22430)
                     /locus_tag="PLV81_02955"
     CDS             complement(21453..22430)
                     /locus_tag="PLV81_02955"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOM09092.1"
                     /translation="MDYFANLKKYLDAHDNFIISTHESPDPDGLGAEIAFKELLQKIG
                     KQAIIINSDPTPSKFSFIDPDNEIIIFNKNMKIDNINNFAVFVLDTNDFDNIGKAYHY
                     LKDSIQDVFIIDHHEGETDKIKSNFIKVEASSTSEIIGNILQHFGVIPSFKAAQALYA
                     GIVFDTGCFKYPKTSPVTFVLAAQLVTLGVKPYEIYQQIYENNSLEVFRLRAMMLSTM
                     EVHYGGKLILMKLTPDMLKETNAPFSEGEVNINLPLSIQGVVASVLIKQDIGGPVKVS
                     MRTKGDLDVAEIAIANGGGGHKNAAGFKSLYDFETTRLKVLEEMSRFFK"
     gene            complement(22430..23326)
                     /gene="glyQ"
                     /locus_tag="PLV81_02960"
     CDS             complement(22430..23326)
                     /gene="glyQ"
                     /locus_tag="PLV81_02960"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOM09093.1"
                     /translation="MYFQDLIANLNSYWAKQGCIIAQGYDLEVGAGTFNPATFLRVLG
                     PEPWNVAYVEPSRRPTDGRYGDNPNRLQHYYQYQVIMKPSPLNIQELYLDSLRNLGIK
                     LEEHDVRFVEDDWESPTLGASGLGWEVWLDGMEITQFTYFQQCGSVELDPIPVEITYG
                     LERICMYIQGVESVFDIQWNETLTYRDVHHRSEFEFSHYNFNIADIDLHFKLFDLYER
                     ECLKILDYEPVQVVLPAYDYVLKCSHVFNILDARGAISVTERVGYIARVRNLARRCAE
                     RYIAIRESMGYPLCKYAQSENV"
     gene            complement(23352..24875)
                     /locus_tag="PLV81_02965"
     CDS             complement(23352..24875)
                     /locus_tag="PLV81_02965"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOM09094.1"
                     /translation="MEGLQQKAQYFCKPANYKEALKMGQDVYIQFSLLTPEVESIIIR
                     MLSVYLAQLDLLYLKDMFINIIKELVNNAIKANAKRLYFKQKNLDINKTEDYRTGMET
                     FKEDVFVENPEFLEQLGQSNFIVRVFFSITGDTHKIHVINNVPIIPEEMNKINARVKK
                     AYSYSDISDAFDDILDDSEGAGLGLIIALMLMKNAGFPPEAFAISTNGKVTSASINID
                     ESFRNMDLKVKMAQEIINEVESLPSFPQNILEIQRLCANPESTIKAIADSIKRDPGLV
                     TAILKLANSAGYITSKRVETIEEAVKIIGTKGINTLLVASGVQKILDSRYKKFETIWN
                     NSYKTAFYAQFIAMKYRKERLSEFAYLASLLADIGLMVLLSIKPDDIQKIRDIVGLKQ
                     LENISLLEEISLGVSHSTLGSMICQKWKFNDALVKAIEFHHRPHLSPPQYRDLINIVY
                     LAYVFSSIETKNFRFEIADEDCLDYFKLNNKHDFEQLHELTKARYDEYIKTNQQIAK"
     gene            25097..26197
                     /locus_tag="PLV81_02970"
     CDS             25097..26197
                     /locus_tag="PLV81_02970"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOM09095.1"
                     /translation="MAKVYFIDMHMSSRENISSKIKKLFYKLEVNSIINEKDFVAIKT
                     HFGEYGNIAYLPPAIIRAFVDCVKDLKANPFITDANTLYRGSRSNAIDHLKNAEMNGF
                     SIFSCGAPVIIADGLRGNDFKEIPASGKWYSSVKVASAIHDADAMIVVSHVKGHELYG
                     FGGAIKNIAMGCAPPSMKQSLHSDLKPRVKQALCTSCGACIKRCPAFAISFDDNKKAF
                     IDSNKCFGCGECTVICIYNAIPVVWKTDATALFERTAEYVNAFMKLKKNKVAFFNFIM
                     NVSPECDCCYWNDTPIVPNIGILASTDPIAIDRASVDLINKAMPLPGSRIAGKDASKD
                     NITALYPVQWEYLFKYATSLGAGDEEYDLIEI"
     gene            26246..26686
                     /locus_tag="PLV81_02975"
     CDS             26246..26686
                     /locus_tag="PLV81_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM09096.1"
                     /translation="MKSPHDFPAINPKKPDGIKPNGIPYKVMVVEDKEFHRKQIIQIL
                     ESEGYEVIAAATNGAEALKLYDSLAADLDLITTDLDMPKLDGYALLYELSQKKPKAKI
                     VFISDETTKGVLADLLKMGAADFILKPIQRGTILERVKAVLQKK"
     gene            26713..27789
                     /locus_tag="PLV81_02980"
     CDS             26713..27789
                     /locus_tag="PLV81_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09097.1"
                     /translation="MEENEKAYQHLSEQTKKINIALHYTHGDMEKARQMVAGNYNDLY
                     AIKVRFASSTMFGAFLLFFNHIYKIISGLTLILSPSYTLQNINPEWDWIVFEKEIAEL
                     MKKGDYDQILAKTFRDKIIKSLDIVLLNELARNIELKNEIAIDNLFRKLIQESLALKR
                     VDSFVVYQPTSSLTMELHSVTIPKLKSEIVDGEQVTKSETQPVATGETEYTPQVGVDG
                     VKLIIKSALILSPIKGKHISKLSPGDRVMISIIDTNPQAVKVAQALNAYENETFKPIP
                     ARITYVKYIPQKGYKVYGLIAKGIVVDIDEEEENIKVALDPAYAATIQSPEEEVKPNY
                     VLIAILLLAFGILMGILYLLLWSK"
     gene            complement(27791..28549)
                     /gene="hisF"
                     /locus_tag="PLV81_02985"
     CDS             complement(27791..28549)
                     /gene="hisF"
                     /locus_tag="PLV81_02985"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOM09098.1"
                     /translation="MLAKRIIPCLDVKDGRVVKGVNFVNLQDAGDPVHNGMFYDEEGA
                     DELVFLDITASSDRRAIILKMVKEVAETVNIPFTVGGGIRTVDDVRMILENGADKVSI
                     NTQAVQQPELITECARRFGSQCIVVAIDAKRNNGGWNVYLHGGRTKTDIDAVQWAKQV
                     EDLGAGEILLTSMDRDGTKLGYDLELTKKVAESVTIPVIASGGVGTLKHLYEGFAIGK
                     ADAVLAASIFHYREYSIKQAKEYLAMRGIPVRPV"
     gene            28666..29733
                     /locus_tag="PLV81_02990"
     CDS             28666..29733
                     /locus_tag="PLV81_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOM09099.1"
                     /translation="MSKKNEYISFAEFSRTYVPAINEYIYTFYSNKELSAHNPDIKLA
                     YNLLKEYCTRDGKRIRPLLLLLSYLGYGGPISEIQSVIPVAASLECMHAFLLIQDDII
                     DGAIMRRGKPAFHKVCDSSYNKYNEHIGSWVAIVLADVLMSNALEMISGLTTTSMSAF
                     LHEFAIIYERTALGQMLDIIWSNPKYIDDENIPHAIASMKTAHYTVSGPLRLGYILAG
                     GSNNDELQKLEQAGYKVGFAFQMRDDIIGVFGVEEDSGKSASSDIEEGKYTVLVQKTL
                     QLLHDSTVKEQFMALFKKKQKSKEDIQAIKAYIEKSGAKANVQQLISRYVNNAKEIIP
                     QLQCDSFMKRCLLDFIENLFN"
     gene            29793..30998
                     /locus_tag="PLV81_02995"
     CDS             29793..30998
                     /locus_tag="PLV81_02995"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM09100.1"
                     /translation="MIYKNRQLEYILKEASTTFPALLLTGPRQSGKTTLLKNLFSDKA
                     KFISLDESDIRLWAHEDPRDFIQNNMPPVIIDEIQYAPDLLTYIKRRIDDDRSPGQWF
                     LTGSSQFTLMKHVSESLAGRTAIFHLLPFSHSEIHGRVYPNWDDWFTSIKDNVPCDED
                     LGNVLLRGTYPELVCNPTINRRIWYDSYIQTYIERDLSLIYNIGDINTFTKMLTLLAA
                     RTGTILNMSSLASDLGVSVPTVKRWISVLESSFILYLLKPYYANIGKRLIKSAKIYFL
                     DTGLAAHLTGIRDREVLLRGPLSGQLFETFVVSEFVKSFYHHGERPALTFINNKNIWE
                     VDLLIERNMNMIPVEIKLSATITENHLKNFKLLRNVLQGLDDSNYLICNRKEYNRMKG
                     THISYWGSL"
     gene            31221..31886
                     /locus_tag="PLV81_03000"
     CDS             31221..31886
                     /locus_tag="PLV81_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOM09101.1"
                     /translation="MVPTTVKKHHKDTFEKISPEKRERIIKIATQEFAKNGFKGANIN
                     IIARKAGVSIGSMYNYFESKEDLLLTVVNEAYKLLEKVLGSVSLENKNFYEKIEELVR
                     AAQKFAREYPEIHQVYLDITSEGLSHLTPRLSKTIEYITIEFYRHELEAAKREGIVRQ
                     DVDINVASLCIDNILVLTQFSYTSEYFKERLKLFIGQDALRDDERIVNGVMDFIKNAL
                     MPK"
     gene            31943..32542
                     /locus_tag="PLV81_03005"
     CDS             31943..32542
                     /locus_tag="PLV81_03005"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOM09102.1"
                     /translation="MKKKNVNIIEVRRNQLTRAAYKVVSRKGYYNFTVRDIAREAGLS
                     TGLVHYYFKNKDDLLVSVLRVMNENLSFYLAKALEQTNDPKEKIIIFMDEAFHLVERE
                     KEYFHVLIDFWTQINHNERMRKANVKLYQSYRAECSKILQEGIEKKVFKDVDVEYTAT
                     MIVAFVQGLIIQYIIDPNAFDYAEYNKKIKQQIIENLIR"
     gene            32626..33567
                     /locus_tag="PLV81_03010"
     CDS             32626..33567
                     /locus_tag="PLV81_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009108066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOM09103.1"
                     /translation="MFKDISENGDYQKHLEHVVQTLCQPESYTKVYHRTNHDVPMPSI
                     PQLAEIVEECKLVLFPGYFGNSEITPQNMQYYIGSALDSIARKLTEQIKRGYCFSCAE
                     GETMDCIECEHRSRKIMFQFLDMLPEIRRMLALDVQAAYEGDPAAKSHGETIFCYPSI
                     MAMINYRIAHALYILDVPLIPRIITEMAHSQTGIDIHPGATIGEKFFIDHGTGVVIGE
                     TTIIGNNVRIYQGVTLGAKSFPLDENGNPIKGIPRHPIVEDDVIIYSGATILGRVTIG
                     KGSVIGGNVWLTESVPPGTHVLQQKPMEMLFENGGGI"
     gene            complement(33632..34597)
                     /locus_tag="PLV81_03015"
     CDS             complement(33632..34597)
                     /locus_tag="PLV81_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 43"
                     /protein_id="HOM09104.1"
                     /translation="MKIKDFLHLTFKEYEGNPIIAPPFPSPIIADPTFVPPNESPDGK
                     WHLFAHSLMGIHHFVSDDGINYTRMSKHTIGNAMRAFLYYEDNTYYLYYEKIKPYILW
                     YSWLPHTWYSYIAMRSSHDLYSWSDEQIILKPSLPWHSNTFGASVSNPCVVKTKQSYF
                     LYYSAALTHIQDCGFNEPLYIGLAQSSNPAGPFQSLPHPIMEPSHYNRWCNIGCGSFK
                     ALKMDDGFIGLQNGIYWDYTINQSGSAILVYSSKDGIHYTSLMKNPLLKPDIKEGSYK
                     RSHVYAFDLKQVDKNHWYLYFNARNNWHWSKGKEHIALLIGSKTS"
     gene            34795..>36757
                     /locus_tag="PLV81_03020"
     CDS             34795..>36757
                     /locus_tag="PLV81_03020"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOM09105.1"
                     /translation="MKPIVTIIILLYAAIALTACTPSVEIVQSLEGTWQLTTTNKDEY
                     CSTVSIDRIPITVPFVISLPHTLEDKKTGNKITLAESGYVWIQKEFVLDTVPAQDVAL
                     QIGEIMNADRAYCNGVFIGASGRFPPHFKSAWNQFRHYTIPATLLHKGKNLLSIKIYY
                     DAEFWINAPLRMVSDSSGKTAFDFINFMRVHLMQAMFFIMLTLAIFFIVMFYYQRDSV
                     EYLFFGLACLSVSFPQLLHFIENLYPDVPYSSNTVLKVSQPGLIFFPALLNLFYRYHV
                     GRNKLWITSIYLLIPVVLTVVLIFQKTRSDILFIRNLSIVVSILFMIDIFVTSGLQLM
                     QHKKAGLLIFIGIVPMVILGLHDVLAFGFKIIPSDVVLYVYGLPFLLFIIAIYLVNIF
                     ITALQQSKELNVQLQQLLQDARKLAYLEKELEIARTIQNQLLPSEIPHLKGMDIAIRF
                     QPALRIGGDIYNIFQLKDGIVIFLADVVGHGIPAAMIASMVNILFKMFQMLSVDPGLL
                     LRSLNINICNMLPQTFVTAACLYINRYEKFVRIARAGHPSIIIQKADGNILEYMPQGK
                     ALGLKTDNKYETLNQAVSPGDKILIYTDGIVEAIDINKNMFGSERLINIVQQTKEASC
                     AESIEAIYSELIHFVGDTSALEDDVTLIMI"
     assembly_gap    36758..36857
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            36974..37648
                     /locus_tag="PLV81_03025"
     CDS             36974..37648
                     /locus_tag="PLV81_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09106.1"
                     /translation="MLKNIGCLLVFVIFLSPADVYPYRPFITEDAGVSGLGEQKVEMG
                     FVGSRQNEYWYNYISLLYGIGFGKAEILVETPYCINDEARGLEGIILAAKINVFEVDD
                     TLMLGAKAGYEFPDDCYGVSAIATKKIGILVMHSQIGLKRAFNDTVGLFGLGFEYQLL
                     QHLSVIIDNFTEYDGDALYHRVLTGVIYSFNSEIALDVATGYCYANNHNNYREYFTVA
                     GISFIF"
     gene            37735..38988
                     /locus_tag="PLV81_03030"
     CDS             37735..38988
                     /locus_tag="PLV81_03030"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOM09107.1"
                     /translation="MKKILIIISVSIISIFTCYNASYAQTHFPVQQLIDNALQSFELL
                     KAQQVKIDELEALKKYNTQWKNPEIAVYYGSKSIDSQKGHVSGIAVTQPLYFPGKLSL
                     IDEIYRYKKNYEVLSYEEMKYFIATSVINLSYEYAISQLRSSHINSRIKRLNLLNTYM
                     TARPLVSPQKKVEVAIVKNSIRMLQSEIYRIKADQAVTYARLALYTQLDSPDLNISTK
                     WIENPPVFDVNDVIARAKNESIMVKKQKEILMASLKEISLAKKNMYPDFNILFNYDYE
                     KVYEKEKSISGGISIPIPLYNQNQNAVDAFQSKAKQEELYLQYIEKMIESDIRTTVAQ
                     YNYYLNLLPLFPPSIENELEQSMYYADDQFQKGTITLQSYLELDVQIHQTLENIYTTQ
                     KELVRSMAAIATFINDYNLLLEIAK"
     gene            38985..>39322
                     /locus_tag="PLV81_03035"
     CDS             38985..>39322
                     /locus_tag="PLV81_03035"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOM09108.1"
                     /translation="MISAWIEKALTGRLWILIVTIGIVIAGFYSIGKLPIDAVPDITN
                     VSVMVNTKTGALAPEEVERTVTFPIESELAGLPDVQDIRSLSKYGLSQVIVVFSDNTD
                     IYFARQLVNER"
     assembly_gap    39323..39422
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <39423..41834
                     /locus_tag="PLV81_03040"
     CDS             <39423..41834
                     /locus_tag="PLV81_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HOM09109.1"
                     /translation="VRTYGKIDSLQQIKNIPVKLNVYGAPVRLWQVADVVVGHQQRLG
                     AATLNGNETVLGTVLMRINANSREVSLAVEKFIKEIPLPSDVEIKILYTRSFLVNATI
                     KTVSRNLTEGGLLVITILFFILGNIKAAFLVALAIPLSMLFATSGMVASGIAASLMSL
                     GAIDFGLIVDGSVVMIENIVRHFNTVKNRSLTKKEKIVIIGDAAKEVSQPVFLGVLII
                     MLVYVPVLSLTGIEGKMFRPMAETVLYALGGSIIIALFLMPVLAYYSLSPKKTDKEPF
                     VFRVIEKVYNPFLTFSLKHPVKVIIPTLLVALISIIMFIHLGSDFIPRLDEGDMVIGL
                     VRDQTISLDSSLEIQKKSDQVISQFKEVKTVFSRMGTPESATDPMGVNFADTFVILEK
                     DKSKWGLYNGKHPTKVELFEAIALKIDEQVPGQEISLTQPIEMRFNEILEGNRADITL
                     RIIGQNLDILYELSNKSIPILEKIEGASTVELDPLTALKKSEIIDVVLNYDAISKYGV
                     NINDVNNAFSIAMSGKQVGYLYDEDKRFPIMIRISDVFRNNVANIAKIPVGLADGGAI
                     PIGTVASVQRHQQITTIARTRAQRYAAVSINIKNRDTQSFVNEAKEKIKALNIPKGYQ
                     LQWGGQFKNLESARLRLLIIIPIVLAIIFLILIRTFKSLTQTLLVFNSIPFALTGGIM
                     LIYIRNIPLSVSAGIGFIALIGIAILNGMVLVNFFNQLRGQGVKLEKAVYEGTLIRLK
                     PVLMTALVASFGFIPMAFNTGIGAEVQRPLATVVIGGLISSTILTLLLLPTLYLWVEK
                     LFGKE"
     gene            42460..42822
                     /locus_tag="PLV81_03045"
     CDS             42460..42822
                     /locus_tag="PLV81_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09110.1"
                     /translation="MKKLLPVLIVLFTTIANSQTYLYELSDNIETYKNKQITMILKLK
                     RYDTVFQVITFYDNKNHDITFDCIPLVKQPWFIQQRLNLHPGMEYTVTFTVKGVGNLK
                     EVIADLNYFEPVILQKLP"
     assembly_gap    42983..43082
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            43136..43636
                     /locus_tag="PLV81_03050"
     CDS             43136..43636
                     /locus_tag="PLV81_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOM09111.1"
                     /translation="MKKMSLFFAVMLVMMMVACSSTPEVQQPAGDPNIALVNAQNQQL
                     EKIPVTGFAYKSAVVPKQSWDKWAEVAAPVVKEILNKLPEGYVLQVTGHADGSGPEEP
                     VGNKPGNIKISTERAKAVYNALKAKGIDSPKMTYKGVGSSELLPGVDPRDAANRRVTF
                     KVVPKK"
     gene            43729..44661
                     /gene="rsgA"
                     /locus_tag="PLV81_03055"
     CDS             43729..44661
                     /gene="rsgA"
                     /locus_tag="PLV81_03055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOM09112.1"
                     /translation="MHGVVSKVMGLYYTVYHDSTYTNCVLRGKLRQKFECDTEKFSNP
                     VAVGDEVEITIADDGTGVIEEIVPRKNYFSRKEKGKHRKIDIIAANCDCVVVMQSFAL
                     PKLNLRFADRIAVRCGQQHIPLILCVNKYDLASKKDIEYIYNYYKNTDISIVMTSAKT
                     GYNIDEFKNLLYNKTSVIVGYSGVGKTSILNTIYPHLDLRVGEVSASTGKGKHTTTNV
                     TMIIVEEHTRIIDTPGLREFGIMDIEPHLVSKYFYEFEKYAARCAFKPCTHDHEPDCE
                     VKRLVQKGVIYEERYISYINILYSLKDYYATMYS"
     gene            44658..45410
                     /locus_tag="PLV81_03060"
     CDS             44658..45410
                     /locus_tag="PLV81_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOM09113.1"
                     /translation="MITAEHVRLTFNHHPVLHDVCFSVPQGLIYVILGKNGSGKTVLL
                     KTIAGLFTHFTGTIALAGIPLQEYINLTIEEKMKRNITMAYVFQKGGLFDSMNVFDNV
                     AFGLRRRNENEAIVTEKVMEALEWVGLSGSEQKLPSQLSGGMQKRVGLARALCLSPSI
                     ILYDDPTAGLDPVLADSISDLIVATQEKYRMTSLLVTQDLAVAQKTAHRVGLLHNGMI
                     VVDLESHEFFSKNNPYAKQFIDGDSEGPIDSM"
     gene            45557..46594
                     /locus_tag="PLV81_03065"
     CDS             45557..46594
                     /locus_tag="PLV81_03065"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOM09114.1"
                     /translation="MQTQREKSKLDWQNLPFGYIKTDYNIRFYYKDGKWSEGELSTEE
                     TIPIHIAAPGLHYGQQAFEGLKVFETVDGRIVAFRPDENAKRMQRSCERIFIPEVPVD
                     MFIKAVDTVVAANAKFVPPFGTGASLYVRPVIYGAGARVGLGPADEYVFIVFVTPVGP
                     YYKGGFKPVKALVVEQYDRAAPNGVGDCKVGGNYAAGLRGGEYAKKKGFPIPLYLDPK
                     EKKYVDEFGTSNFIAIKGNTYLTPESNSILPSITNKSLMDIATLLGMNVEKRPIAIDE
                     VEHFDEVGAVGTAAVITPVCSIHYRDKVFTFCDEEKAGPTITKLYEYLTKLQTGEIND
                     TFGWLHEITVK"
     gene            46623..47042
                     /locus_tag="PLV81_03070"
     CDS             46623..47042
                     /locus_tag="PLV81_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005991067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HOM09115.1"
                     /translation="MTNQDIRWIQRFNHFMNAFKRLEDAVTLYNDRTLSDLEEQGLIQ
                     AFEYTHELAWNVFKDFLEDRGVTNLYGSKDTTRYAFKNGLIDNGEVWMDMIISRNLTS
                     HTYDEELAKEIIKKIVNVYIKEFDKMFLKFNTLKNEN"
     gene            47046..>47317
                     /locus_tag="PLV81_03075"
     CDS             47046..>47317
                     /locus_tag="PLV81_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOM09116.1"
                     /translation="MKFGLSEKTIDLICSIFRQYPQIEKVIVYGSRAKGNYKNGSDID
                     LALIGGDDLTMDVLYKILHELDDLLLPYSFDISLYKFIHDPDLIDH"
     assembly_gap    47318..47417
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <47418..48854
                     /locus_tag="PLV81_03080"
     CDS             <47418..48854
                     /locus_tag="PLV81_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM09117.1"
                     /translation="HHEAGKNFDELVVKIYNFSLIVRKAIKKKSVLFLPTAKPFQHDE
                     TDISVVIIPLLHADIVVGMVCLVFPLEELQYLQNNAPFFMLIGKITGTIRYYQTGLKV
                     SKSDTAEEEFEKTIASLRVLAQGVAHEFNNILAVIKGYAELLLMNEAVDESVKKAVSI
                     IDKQTTRGSSLIDRLSVFVTGKEISFEYCSLNDLIKEIVDLQRFTLEKESIECKLKLG
                     KVPEIFVDSRQIKEVLLNLITHARSAIKPNKKGTITIQTSFNRDAVMLKLVNDGVPVN
                     WENGGEQKAKGSNGVDSVGNGINLAIAYGIMQSHGGDMTLCHDPARCNEVTLYFKRFN
                     PAFDAVGKKEDKEMLYLGEVRILIVDDEEPIRQFLSSAFERMGYQVLTTDNGQEAIEI
                     CTFEDIDIVFLDYLMPGYKGDAVFKSIHEVSPQTDVVFITGVDSIPDIDKLLMMGLEG
                     VLRKPFKIETIIKLTNEIMARRYNTLQD"
     gene            complement(48858..49055)
                     /locus_tag="PLV81_03085"
     CDS             complement(48858..49055)
                     /locus_tag="PLV81_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09118.1"
                     /translation="MAKPLLGELLVEDGVITQDQLNQALSIQKKEGGLIGIILMNLGF
                     IDEPTLVKYLALQAERVIKSE"
     gene            complement(49117..>50112)
                     /locus_tag="PLV81_03090"
     CDS             complement(49117..>50112)
                     /locus_tag="PLV81_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOM09119.1"
                     /translation="TIDTWILWKLTGGKSHKTDYTNASRTLMFNIKEKIWDLDILEIL
                     NIPRTILPEVRESASYFGETSGVPGLPDGITIGGIAGDQQAAMVGQNCVFEGTSKNTY
                     GTGCFMLLNMGNEWMLSKHGLLTTLTCDNKGQPVYAFEGSVFIGGAVVQWLRDFMKFI
                     EKSSDAEKVSLSVKKEDEVVVVPAFVGLGAPYWKMDARGAIFGLTRDTTPQQIVRAAV
                     KSIALQSYEVLEAMQNDSGKNIHELRVDGGATRDTFLMQYQADILGIPVLLPEVTEST
                     ALGAAYLAGITEKVYNTIDEVAKFNKIAKRFNPSMNETERKREIALWKDAVKRLL"
     assembly_gap    50113..50212
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<50213..50340)
                     /locus_tag="PLV81_03095"
     CDS             complement(<50213..50340)
                     /locus_tag="PLV81_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HOM09120.1"
                     /translation="MGKKFLIAIDLGTTGNRVFCFDEHGLPISSSYAEFTQHFPKPG"
     gene            50680..51723
                     /locus_tag="PLV81_03100"
     CDS             50680..51723
                     /locus_tag="PLV81_03100"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOM09121.1"
                     /translation="MDLFEKNYLNKLQNNEPVFIAEIGLNHNGKLDEAIALIQKAAQA
                     GADLVKFQVFNAEKFYSVYTKSLLNHEKPKYDDCSIQFFKKFELSFNDYRTLQHVAYD
                     HGVVFFASCFDDDSFAMMENLNVPLYKIASSEITNVPLLQNIVATHKPVIVSTGISLQ
                     HEIAHAIHLLQKEQCNVVLLHCVSLYPVPHDAINLLRIKRLQKLYGLPVGFSDHTKDN
                     RASMMAVAMGARVFEKHFKLSHNHDCADKDVSLTPEEFTAYIEDINMALAMLGDGSID
                     YKEGEAAVARSARRSIFAARDIPAGTSLNYSDIIVKRPGVGLSPLHSDAIIGKKVKHT
                     IHKDMPIYGEDLE"
     gene            51987..52517
                     /locus_tag="PLV81_03105"
     CDS             51987..52517
                     /locus_tag="PLV81_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09122.1"
                     /translation="MKKIAIVFLFFIVVTPAYASTIDYIFNDNQRLILNSATDIIQAS
                     LGYDDIRELVYITFWSTQPADAKKIDTFNMYIAQQYKTSPDDVIFIYERLLQSVYMIE
                     YMADLAKSKKKWKFYYYYSDTLLPDTRRFCDTLKQAIIKADPSIIEHINKRDVRIKSY
                     AIDIVTYREALYGGGF"
     gene            complement(52638..53420)
                     /locus_tag="PLV81_03110"
     CDS             complement(52638..53420)
                     /locus_tag="PLV81_03110"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOM09123.1"
                     /translation="MKTKKIKRIQSIERAHAILSLFINDKKPLGISDFARQLTLPKTT
                     IQGIVQTLCHLGYLEKDHETGKYRLGPYVFQLGMKYATNLDLVSIARVWAERLCFQFR
                     QPVNIGMLVGDKVVIVLRVEPENRFMSYPQAGSVIPTHTTCIGKLLCAYMEKERLEEI
                     LKDYAFQMLTPNSITSKEDFMKEIKKVRQAGISFDNQESVMGLAGIGGPIFNYSGIIA
                     AFAITGNADFILKHKQDIIHAVRDTSLQLSVQLGYNPDSKIL"
     gene            53544..54650
                     /locus_tag="PLV81_03115"
     CDS             53544..54650
                     /locus_tag="PLV81_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOM09124.1"
                     /translation="MDILKKFEITDPYEYVANLKKATGKSVLAYMCSYTPEEIIYAAG
                     IHPFRIFGQVETISKADAHLQAYSCSLVRGALEDALQGRLSFVDGAVFPHTCDSIQRL
                     SDIWRLNAGMKHHFDVVWAVKLNTDSARKYMRDVLVRFTKELEQSFGISLSDDKLQKT
                     IATYNEIRSLLKTIWQLKSDNKSALTGSQMNAVIKAAMVLDRDQLVVNLKELIPSIEL
                     SPQTEKRKRIMLIGGICNHPDIYDYIEASGGVVVWDDLCTGSRYFEGAIATDKEPLQA
                     IADRYYERNICPAKHRDYYTRAETIKAMIKKHNVQGAIFLYLKFCDPHSFDYPYLKEA
                     LDEVGVPSILLELEQQLPSEGQMKTRLEAFIDMI"
     gene            54669..55898
                     /locus_tag="PLV81_03120"
     CDS             54669..55898
                     /locus_tag="PLV81_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HOM09125.1"
                     /translation="MAEPQEKKDNRIKSVKRMKEIMTNYYIEAKTAAQNNKKVAWITS
                     GGPVEPLLVMDVIPVYPENHGAMVGASKMGVQLCEKAEEMGYCRDLCSYARSDIAAAP
                     INGGPIGGLPKPDFLVCCNNICGTVLKWYEVQARYFNVPLFIFDTPFCHSEMTQEAKD
                     YVGAQILEYIAFLEEHCGKKFDYDKATEVGKLSVAGQRLWQAVLDTAMHKPAPMSAFD
                     AFFHLALIVTLRGTQIVVDYYTELLNEMKERIANGISAIPNEQYRLLWDNLPVWYKTK
                     WLSDTFAAHNACLVADTYTTAWCRQLDYLDENDFLKSMVEGYTAIYLNISVEQMAKAV
                     IGMIDKYDVDGLVMHSNRSCKPYSLGQYDIQRIVQKERGIPSLIIEADMVDERNFSEA
                     QVLTRIEAFMEIIKSKK"
     gene            55919..57637
                     /locus_tag="PLV81_03125"
     CDS             55919..57637
                     /locus_tag="PLV81_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOM09126.1"
                     /translation="MYQTKPWLKYYGDVPHSLDYPKVTMYEALMRTVERKPNAIAWDF
                     MGYTATYKEFAQLIDRCAEGLYALGLKEGDTITISMPTTPQGIICFYAVNKIGAVASM
                     IHPLSPPREIEFYLTISNSTFALTIDAFYGAFKEVMDKTPCKTLILTKISDFLPGLKA
                     LGFYLTKGRKIPKVPKDPRVVWWSDMMKKSSKVPKSSMKTDDMAVILYSGGTTGMPKG
                     IMLSNMNFISEGMQVASWGKMSEDDSILAILPIFHGFGLGVCTNAFFMGGGKSILVPQ
                     FDPVIVAKLIKQKKPNFLIGVPTLFEALSKSKVFGSADLSCIKAAFSGADTLPRVVKE
                     EFERVVARQGGNVKLREGYGLTEAVTAIMAMPMNEYREGSIGIPFPDMMAKIVKQGTT
                     EEAPCGEEGEICVAGPAVMLGYLNQPEETAKTLRKHDDGLVWLHTGDIGTMDEDGFFY
                     FKLRLKRMIKSSGFNVYPSQVEDVLRKHPAVDQACVIGVPDKAQVERVKAFIVLKPEY
                     KDKAGSALAEELIGHCREHLIKWSCPREIEFRDELPKTKVGKIAFNVLEEEEKEKLRS
                     RGEYAG"
     gene            57656..60037
                     /locus_tag="PLV81_03130"
     CDS             57656..60037
                     /locus_tag="PLV81_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09127.1"
                     /translation="MNRLSIYIFEFAFRFPWLRYIVWAIIVSVSIYAGYHLFYEEVVT
                     YPVGWERSAVITPYTLKVKSPFAVSKGNVVAVVFEGTEKNEHGIYIILSLNGGKDFLP
                     PYKLSDIDPAVAVRPHVAISNNGHIACTWHNLVDSKSNIFLTTSNDLGATWTKPVALL
                     LQTDESMLSQVFYDSTGGLHLFYHGSVGGRLILYHAFSPDELQFTRIDTIADIATEYR
                     GIFFPAIVFEGDSIYLVFQAKILSGGVLSDDLFFVYSNNAGKSFSNPRRITTSPANDS
                     SPSILLHNDVIYCVYQNNEKRQWDVRLLKGYNRGKEWEQQPVIVTDINVDCYNPRIVA
                     SSDDELLVVWHDMRERYPAVFGRKVSLIDNSMTAEVRISDANVTSYDQFPVVIKKQVL
                     LFWNSNQRLMAKFSDISVLPPNVYSRTHPENVWTRNTNAIIEWDPPVDESGIAGYAVI
                     YNDLPTFDPTVQNVEGPIRKYVVPDLKDGITYFHIRAIDKAGNYSRTIHYPLLVSSTP
                     LPMPTVSSPTHPEATQVDNPVAVVQWTIEQRDRLKGFYYAIAKDAIVEPSTFTTADSV
                     TLESLPKGRYFFSIRPVDMTNMKGRMATYEIVIGDVGKADAAYYERLAKGVLPPEELP
                     EKVPGKVRIQYPIVLFNIPFKEIYASSFYKFSLLPKNILPKQVQGYSVVVSSQKVVPP
                     FIVTHPTSGVMLTNVKPGSYTITARMRYVYYQNGKAVYKWSAPSYTTLVVKSAYTSPL
                     MNYAEHLNAMIKDRQVVTIIYSIGMLCIVLAIGMSTRIAFSFKSLWYRIFRIF"
     gene            complement(60051..60380)
                     /locus_tag="PLV81_03135"
     CDS             complement(60051..60380)
                     /locus_tag="PLV81_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09128.1"
                     /translation="MKRYNSTPNRLSYTSGKYSPPAKRYTSNYLIPLIILVIVLTITL
                     FIYRSCNNNSNHQTTATHHIEQTGNSVNHTKTHGKTVHKKQNIPLDTSPEDSLPPETD
                     LRILTDK"
     gene            complement(60402..60824)
                     /locus_tag="PLV81_03140"
     CDS             complement(60402..60824)
                     /locus_tag="PLV81_03140"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM09129.1"
                     /translation="MAHSKMTLLKAKYPTIKEIRKSIVDDIITELAKNKVQVKLDNLE
                     LYLIIDEALTNAMEHGNKWDQNKYVHIEVAKDTNQLYVTITDEGKGFKYPDSHSTVHL
                     QPRGRGIFIIKQFAKVSWNKKGNSITMAVPISYNSNPV"
     gene            complement(60853..60927)
                     /locus_tag="PLV81_03145"
     tRNA            complement(60853..60927)
                     /locus_tag="PLV81_03145"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(60890..60892),aa:Pro,seq:cgg)
     gene            61492..>64007
                     /locus_tag="PLV81_03150"
     CDS             61492..>64007
                     /locus_tag="PLV81_03150"
                     /inference="COORDINATES: protein motif:HMM:NF039580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOM09130.1"
                     /translation="MKKNDGVITSGIYDGIIYERLNPLLEKLALVLFNTVDFDEESCS
                     RIEECSRKGHVVYASFQSSNLSLLILYNLLRRHNYPLPVFALEYNPFWFQDIGFITRR
                     LWQFFSSTVLGKQYEYVLDTDYVETLIKDNKSILFSLLSKRYFLKRYTEVKYDSLRYL
                     IEVQKRIDEPIYLLPEMIFWNRNPERSGSPLGPLYSIISSQATGDKSLLFAWLTIMKS
                     ATAPFVRISQPINLKDEIAKAKVDDTRQLAVMLRNKLLEDYYHEKRTILGPVLKSQQE
                     MMERVLYHPNVENEIKRIAQEDRIPESKLRKKAYKYFKEIAANFSILYIRVFEMALDW
                     MFRKIFDGIRYDPKAIEEIRQAATKGPLVLTPCHKSHLDYLLLSYVFYKNKMAPPHIA
                     AGVNLSFFPMGTIFRHSGAFFMRRTFRGLKLYPTVFKQYIKTIVSEGYTIEFFIEGGR
                     SRTGKVAFPKLGFLSYITEAIEEGYNTDVVFVPISINYDRILEESSYVQELKGKEKEK
                     ESVVSMMQSSKLLNRKYGMVYINFNKTFTLKEIKTTLNPVNVPESVAMNIVRRINDVV
                     TITPFSLTTTAILMSSVKSFSREMVKNNFESLYDFCEYKGYQFSDTLKDRNNFDEIID
                     YVIQSFREDKILESLKVVGEGKKLETMPDMYVLPEENRPRMIFYKNSIIHYFLPLTFS
                     SLALRIMNKKGETSLDSFRSEYVYIRDLFMKEFIYPENMLEDVMLSIKDAIKYMQQKG
                     YATITKNSIAINDDREKDAQLRFFGRILQEYLESYYVVCSTLLPKKRVQIQKKDLLTE
                     IRKNGVKMYHTGEIMLSESLSLPNYTNALSLLRSEKALSE"
BASE COUNT        20591 a        10380 c        12397 g        20139 t
ORIGIN      
        1 ctctacagtg tcagagcaat caaattatgc aacaataaaa ttaatagagt taaccatatg
       61 aaacgtgtaa tttatgtcat atgcctgcta ttttcaattc aatcttctgc agatactatt
      121 gataccatta taaacaattt tgcggtggaa agcattgatg ccaccattgt acaggttata
      181 gccgaaacag gaaaaccaac tatacgctac gaaggtagat acagggcata taaacagaat
      241 atgcgtatag attacagcaa accgtttccc cagattgtaa ttgtaaaaga aggtaacctt
      301 cagtggtact atcctgaatc aaaagaatta tgggttatgc atactatgaa tgctcctgtt
      361 acgcatccct ttgcacaatt ttttgctttc aaggatagaa tcgtaataaa aagtaaagaa
      421 aatacttact atggtttatt taaaagagca tataagtata tactcataga tactactacc
      481 agaaccagga ttgaggtgat agttgataaa cataaaggat atccggttaa aaaaattacc
      541 tttactgatg ataatgttga gataatgagg gaacaatatg aagattatgc atatatcaat
      601 aatgtatggt ttcctacaac agttgaagtt gtggcacgta ctcgcaatgg gattactcgt
      661 aacataacac ggtattatga tgtacacctt aatatgaaaa tacaaccgga tatttttatc
      721 ctgcaattgc ctaaaggtgt cagagcaaag cagatgtgag ggtattgtga ggcaactttt
      781 ctttcttctt ttgtttattt tttattcaac cattggtatg gcaaaagttg ttgtattagg
      841 tcttaccgtt cccataaaca ttgctgatat tgaaaataaa agcgttgtat cagaacagca
      901 agcaaaagtg ctggcgatag ctcttaccaa taagctccat gaatcgggat acaccacaag
      961 ctatgtcaac cgttaccagc ttgagaacga tatctgctat ctgtatgttg ttgaatcgca
     1021 tattgcagat atacagcttg ctgcagattc cgctttgaaa gaatatatca tgcaggattt
     1081 acagaaacta ctgggagcag tatacaacag gaaaacagtg catgcatttt tagtgcagct
     1141 gaaagaaaag tatttactgg atttaataca gataaatgta gtcaattata atgaaagtga
     1201 tgatgtaagt ctggttataa gggtcagagc caggaagatt gcatggtcgt ttgaagttgc
     1261 cacacttcct atctatggga taactccttc aatagtggta acggtgccca tgcataaagc
     1321 tgttatgaca atgcaagggc aggttggttt taatgaagaa agggttacat tagaaaaagg
     1381 tgtatttgag tatatgagct ttccagcaac aattggattc catgcgggag tactggtttc
     1441 acgggaatat acggtgtggg agcgctatgg aactgatttt acagttacta tcgccaaacc
     1501 gtatgcgggt attgggatat ttaaaaacag gggcgttttt gacatttcag catttcttta
     1561 tggtgtagca acatactaca aggtgtcaga gcacaatacg gcagaacttg aggatacata
     1621 taaagatgca ggcatagaac tgcgaatcca gtctaaagat gatcggtcca ttgcaaaaaa
     1681 aaataagaca ttacaggcat cactgtttgc cggcttctca caggatgaat atctgctaac
     1741 atcacgcgcc ctggggattg caccattaca aatcacatcg cgtttctatg tcatcccatc
     1801 agcttatagc ttttacacaa attcatttaa aaggatatat accgagtatg tgttcgatag
     1861 ttacctgtta ggatttgcca gccgttatac atccacacag tcccggcata tagctaaatt
     1921 agaattaatg aatgaagtga tatatgagtt tgtttttttt gaaatttttg gtgcatccgg
     1981 ggtatataaa tcagaatata acaggtggga aaagacatcc agctggggtg ctggtgctga
     2041 tatatactat aatacaataa taattaacat cgggtgtgcc tggaatactg ataagcaatt
     2101 tagccagtat tacattttta caggtgtcag agcaaaactt tgacaggtag atacatatgg
     2161 tattaactat tgagaataaa caatatcacc cgaatcgtta aaatcataat gcgctatcga
     2221 tgacattttc tttctaaatt tatcagattt aataattttg agaagtgatt gaattgcatc
     2281 attaaaaaaa atttcttttg taactatcat atcaaaactt tctctggtaa gtgggataaa
     2341 cggaatgttg aaaaaatttg ctatagccac cgaagcaatg cctgcatttg cctcgccgtt
     2401 ttggattgcc agtccaactt caatatgtga gtatttaaca ttggtgtaac ctttgacctt
     2461 ttgaggattt atattatttt ccttcaaaag ataatcagtt agcactctaa tccctgaacc
     2521 ttcctgtcta tttataaaaa ctatatcagg atgaataata tctcttatgt tatgcaaaat
     2581 atgtgttatt ccaggagata gaactatccc aatttttctg taaaacagat gaattatgac
     2641 tactggtata tttgtgcata tctcttgaat aaaagggatg ttatatgatt ttgtctttgg
     2701 atcatataag tgtgaccatg caatatgagt aactcttttg ctcagtaaat gtattccacg
     2761 taaacttcct gttgaacagg taaatataga gtgtaatgga ttaatctctt tcaattcgtt
     2821 tagtaatata tctactaccg gatcattact tcctgcggct aacagcgcat gtcccccata
     2881 ctctgtttca ggttgtaatc cttttatatt aatttttgat tgagacagta tccattcatc
     2941 aaccagagat tttggaaaaa tccatttccc tgtaattttt gtgcacggga ttttattctc
     3001 ttttattaat acataaacct gcttttcgtg aatgtctaag tattcagcta cttcttttgt
     3061 attcatcatt tctttgggca tttcactacc tccattgata agtctttgaa aagtattaat
     3121 tacaggaaaa taatgtatgc ataatgtatt gataaaacaa tttcaggata catgacgttt
     3181 gtaccgcttt gcatgtaagc ttgtatctac catatggcat tgagtcaatt ttttgcatta
     3241 ataaaactat tgtacaatca tgagattact acagttgctt tttactacag gttggtaata
     3301 tatgtagttc ttgaggaaca gtaaaagcaa gtaaaaatta atgaataatg tgataattcc
     3361 tgaaccataa aaaagagtaa aaaagtacta ttaaaaagta ctattaattt aatgaagaaa
     3421 acattcagtt gaaatgggat tatcttaaac catacatgag gattacaaaa tgagtattga
     3481 tacattcgtg ctcattcaaa tccatacagc attatgcagt tattgtatag ttttaactgt
     3541 gaataggaat gactaaaaaa aatattgcag tatgaaagat gcaatcaatg ttgctgtaat
     3601 taaacgcaaa gctactaaaa ccatagtgat aaagaggctc acatatagac gctttgttaa
     3661 aaaaaatcaa tgtatgtaat taatgtaatt aatgctggaa aaattcatta gttttcttta
     3721 taaattaacc aataatacaa tgacataaga actataataa cagtaaatga taatacccac
     3781 aaggtatatc cagaatcctt tcttgcaaga agataaaatt tgacctcgtt aaagttgttt
     3841 gtcatgggtg agggagcatt ttgtttattc ttttttttat tttgaatcac aaacaacgta
     3901 taatctttat ataatacagg attaagtgga gaaattgtta ttcttttttt tacatcacca
     3961 cattcaacca ttgtaagttt aatgtcctta atgcaattta taaatagtga cctcttggga
     4021 taatatgtaa ttaatatttt ttcaatataa agattttgat tattgccagg ctttatactt
     4081 tttccttctt caatggggaa cgtatatgtt ttgctgaatc catgggatat cccatgcgcc
     4141 atgagtattg taatagtgaa aatatggata attgaaatgc aatatttgcc aattaattgt
     4201 tttgttgaaa ttaacccacg ttttgtgcaa agtgtatata gtttctttac agtacaaaaa
     4261 aatgtattta tcattatgaa agttacagta atgaataatc ctataatcca ccatacaagg
     4321 aaaaatttat ccaccatgtt gttcattaac caatgaaata tattgctata atcaaggtcg
     4381 ctgaaaaatt taaaatattc attggtataa agcgatcctg aaaatagcat gatagaagtt
     4441 attattaaca gataaaatgt actattgata ctggtaacaa tgttccacag tgttttcata
     4501 taatcagttc cctatacgtg ggatgttata ttcatgaaga tatgagctga tataaaatat
     4561 aaatgttatt agtgaacatg ccagagcata tacggatttt tttctattag tgaaatcagg
     4621 gatatatttt agatgcaaaa aattaatgta gagtacccat ataagggcac tgtgcaggtg
     4681 ccgggggctc catttaaaat atattcccca tccggcataa caccatagtg caccaacaaa
     4741 ttgcgctgca ctatacagaa ggaatccata tataatacat ttatatgaga aattgaaata
     4801 gtcgctatct gtttgtaaaa gatataaagc tgaatagaat ccgcttatat agaataaact
     4861 gatagcaagt gattcaagca taaaaaagat atatgcaaga taggttgatt tattgggtga
     4921 aggtggtatt atgccttttg gatacaacag cgatagcaaa acagcaataa tgaggattac
     4981 agttgcataa aaaatggtat aattgttata tcggtaatag agcaaaataa gaattaccgc
     5041 aattgtccat ggagttaaaa aaacactatc aaaagcaccc tgcaggtatg gatatccgct
     5101 cataaatatt cgtaaaagca gatataccga atgagtgatc aaaccaatga tgagcgttac
     5161 aagcgataac tgctcttttc cagtaactat cgccgcaaaa gatatgatgt aaagaatagc
     5221 agatgcaaaa agtacactat gtattgaatg tatcattttt agtattattc cccgggtaat
     5281 aaaacaatat gtgataatcc ccacacacac ctatctacaa aaaaatcgcc ggttgaagct
     5341 accataaaat ttccctgtgc tgatttccca tttatatgtg ttacaatcat tagtttattt
     5401 cccgcatcct gttcaaatat ctctttatac gaaaataagc agcgatagcc atcgcaccca
     5461 ataaacataa accatgcatc gtgtggaata tatgaaaaat tttgataaag aaatgatttc
     5521 agattaaacc cttttacctg cactatacct ttatatcctc tgccatgacc aacctgcacc
     5581 caatctgata ttgtttgctg gcttacaccg gtaaaagtag ctctatgact tttatctggt
     5641 gaaatgcaga ggatgtatga tgaactgcat tgtttccctt tttctgtttt aggtaatgat
     5701 gaacagtcat attgggggac agtcaaatta atttcaacaa tatcatcaag atatcgtgaa
     5761 gtatccctgt cacaggcaat gacaagcctt aaacccttta aatcttctct atatagattt
     5821 tttgaatacg tatttccttc tttggcaggt tgtattggct cacgcttata tgccagtatg
     5881 tagttaaaca tattgcccga cattaccagt tcgccataac tgcattttga aactttacca
     5941 cttctggact taaattgtac aataatatca tgtgggccgt caaaaactgc atcggcaggt
     6001 tttttgattt tgattccttc aagaatattt aaaagcggga taccatgata tcgataggca
     6061 cctgtatatt ttcccgtatc agtcacttca cgggtaaata catatgctgg cgcaaaagct
     6121 gccagggatt tactggtcaa tgtgtatact ctggcagtat atccggtaat agtaagggaa
     6181 atgccttcag gtagcggggc tacaattgct tttggagttg caccggtaat ggcaataaca
     6241 taacgcgggt tgttcctgaa ataatgcaca catactatgt atagtgcaag tatacccagc
     6301 agaagatatg gcaaatattg ttttaaacgt tccatggtat catcctttta ttgattattg
     6361 ggctatgaaa atatgtacag ggactcagcg ttaactttat ccctgtacaa taaaaaatag
     6421 agatactcat atgcaaaaac taaaactatc ataattcggc attcaatcct atatagaaca
     6481 tccgtcccgg gccgggatta aacggatcag ccgcataatc atccaggata tttttacaag
     6541 taacatacaa taaataatga tgtaaaaatt tttgttgcag tttaatattg agtattagag
     6601 gattatgcaa ttgtactgta tcatatgcat cagcgctaaa cgaagttgag gtaaagtcaa
     6661 aggaagtatt tgccacatac acaatctgat cagcagtata ggttgcccag aaaatacatt
     6721 cagtttgagt gggaaaagct gcatgcagtt gtgctaaaaa ttgatgtgtt gcaacacctt
     6781 caactctttt tccatacgta agactcgtta ccgcatcatt gcgtgcataa gtatatacgt
     6841 agttaagtgt acactctata tctatagtgt acagtgatgc tttatatttt attgtacttt
     6901 caattccctg ggctgtcatg ccatcaagat tcttgtattt ttcataggca ctatcgggat
     6961 cataaatgcg agctatctta tttttaactt ttgtataaaa atagtcattc ctgaatgaaa
     7021 gtgtattttg taatactgta atttcaaaac cggtattgct gctgtatatt gtttcaggtt
     7081 ttatctggta ttcttcatta ttatccagta tatcctgtcc aatatccttg tactgtgaca
     7141 ggttgggaaa catagtcttt atgccaccgg caaacctgag agtgaggaag ttggggacgg
     7201 cttcatacat ggcggatgca acagggttac atgaatcata ggttccccat aaatacgaat
     7261 cctcatccat ttccggataa tgtatgagcg catcagttgt tttatcatat acgcgtaatg
     7321 ttgataattt ttgtgcatcg tacgaagccc caaaagataa aaacactatt ccggctccgg
     7381 tatgcacatc ccattcgtct tcaactgcta tgcttatgta ttgagctctc atgtcagcaa
     7441 ctttgtattc atcagtgccc agcgttgttg caagttgcga tgtttcatca agtgcttttt
     7501 caaatttatc atgttgttca ttacgaaaat ggattgaagc atagagtgta ttgtttttcg
     7561 atagagcata ttgtggcaat atatatgcac catatgacgt ttcttcaaat attgattgcg
     7621 cattaccgtt gtaatttgtt gaattaggaa acatctgggc aattgtataa tcctgattga
     7681 agtagccttc caggttattt ctttgtttca cataaaatgt attgatacgc aatttcagat
     7741 tgccaaatct ggtggtcaaa taaggtgata cattgaagcg ccaatcctca ggccagtaaa
     7801 atgctctatt gctgaagata tctctccatg atctgccgct tgagtctaca taatcgcgag
     7861 tatatacagg gagtgaccac acattgtttt cttcatcaaa gtagtggagg gaattattct
     7921 gaaatgtatt tgaatacatt tcgttgcgat agtagtttgc cgcaattcct atctcggtag
     7981 catcacttag ggagtatcca attttgcctg caatagtatg ttttgtatat tcggtattat
     8041 tccatttgcc ggtatcattc aggtaattgt caactgcatc taaatcaata tcactaaata
     8101 aaggatgtac tatagtaact gccgttccag agttactatc tgctaaatca tatgaaacta
     8161 ttttatcaaa ccactcctgc cgcttatttc tggttagttt ctccgatatt tcatatcctt
     8221 cagaatattg acgtgatacg gcaatccatg tatagaagtt accaacaggc agtcctgctt
     8281 ccgcgcttat aaaataattg ctatactcac catattcagc ttttgctttt gcatacagct
     8341 cttcgggctt tttggtaatt atattgattg acccggcgct gcctaatgat ccatataacg
     8401 ccgatgaaat gccccgatta acaattatgc gttgcacatt accaacgggt atcattgaaa
     8461 tatcaatgtt cttcccacca tagtacgggt caatgacggg aaccccatca ataagaattg
     8521 ccacatattg atgatcaagt ccccgtactg aaagaccggt aaagccttta gcagtctgtt
     8581 caataagaac gcctggaacc atttgcagtg agtcagacag tgctttgtca ttgcgggctt
     8641 caatgtcttt ttcagtaatc tgggtgtttg ttgttgcttt ctcaatattt gaaatctttg
     8701 tttcgctaat aactatctca cccggatgga acactttgta cctgggctgg gtgctttgag
     8761 tatcaatctt ttcttcatac tcttttggct gttgattatc agtttctgta gtattctggg
     8821 caaagcttat tccggttgat aatactatga cgtataatgc cagtagacgt atcactatct
     8881 tttccatagt aatcctcctc atatgcattg ttttctatgt gccagcatgt gttgtgagtg
     8941 tgatttgtca atgattaatt gtaatagtaa gtaataataa gtaataaaaa gtaataaaat
     9001 gtaattataa gttttataac cattatggaa acaatacgta ctatgctatc cgtatataat
     9061 tagttatgat aagctttaaa aacatgacag taattatccc aactttattg atttttatgg
     9121 tatatttggt atacatataa tagggggtat actatggaaa ccgaatgtgg gatagtcaaa
     9181 agagtggatg gcaattatgc ggtgattgaa gcggatgcag tttctttttg ctctacatgc
     9241 gaaaaccaca catgtaccat gcggcaatca agagggcgac agatcatgat agaaaatact
     9301 ctgggagccg ctaccggtga cagggttttt tttgttttcc cttcaaaggg gatactgctt
     9361 tcatcggttg tgctgtatgg tatacctatt gtttttctta ttgcagggat aataattggt
     9421 gcagttttgt catggccatt tcacgataag gatttttccg gcatcataac gggaatggta
     9481 tttttaatat tttcatttgg tattatgcgg ttagcatcac agtatattgt gcagcatagg
     9541 cagctaacac caacaatggt taaggtggaa aagccaaaat aaaaaattgt agacataaaa
     9601 tataaatcat tatagcaaat gcttattatt ttttatggta tatgtgcgat agttaccggt
     9661 tatttatctt ttgttaaaat agaaaggaaa acctttatgt atatttttaa aaatgttgcc
     9721 cgtatatcac cggaattgaa atatcaacga tagtttttac atttaattta ttgtaataat
     9781 gaaggttatt tgctatttcg ttaaacaaag cgttgtgagc tctttacatt gtcaaaaaat
     9841 ttttttcact atttatggaa gtgaaagtaa ccgtaaagat ggttatgaaa ggaaaaagtg
     9901 attaataagt tttgagctat cagattataa cgaacataaa tagagctatc aagcaggagt
     9961 gttgtaagta acaaagtatt tttatcgaca aaaccttatg aggagcagct attgaaaaag
    10021 aatgtgctat taaatggtaa aaccccatca ggggtgataa tatagttatt atatattaat
    10081 tataacggag aaaaattgta tttaagagga gatatcgtta tgcaggtaaa tattgaagat
    10141 aagaaaaaac agataaaaga agaaattgaa aaactacaat acgagatgaa aattgaattg
    10201 ccacagagga ttgctgaagc acgcgcaaaa ggcgacttga aagagaatgc cgaataccat
    10261 gctgcacgtg aacggcaatc atttgtacag gcacgaattg cttatttaag caagcaattg
    10321 agtctgatgg atagcattga tatctcatca atagagcagg gtaaagtgga gtttggttcc
    10381 cttgttacgg tacgcaattt agatacgggt gaggttggtg aattcactat cgtaacacca
    10441 gatgaagtgg atgcttcagc cgggaagatt tcgctctcct cgccaatagg aagagcttta
    10501 cacaaaaaga cagtgggcga tgaggtaaca gtacagatac ctgccggtac caggcgtttc
    10561 aggattgaaa agcttataac catacatggt gaagaactat cgctataaaa aaaggaaata
    10621 ttcatggcta taattaaaga atttactgca ctgtgcccta accaggaact tgtatcaaag
    10681 gtagcagagc ttccatatga tgttgtcact acagaagagg cgcggtcact ggcacagggg
    10741 aatccttaca gtttttttca cattacgcgg cctgaggtag atttacctgc aaacaataat
    10801 ccgtatacca atgaagtata ccggaaagga aaagaaaatt tacagcagtt tattaatagc
    10861 ggtgtgcttt tatatgatga tgctgcgtac ctgtatcttt atacattgac aatgcaaaat
    10921 catcaacaga ctggtatcgt ggcaggagtt tcaattgacg attatattaa tggcattgta
    10981 aaaaaacatg agttgacccg tgaagataaa gaaattgacc ggatgaatca tataaatatt
    11041 gtaggggcac agaccgggct tgtctatctt ttttataaaa acaatgatgc tttgcaagag
    11101 ctgatgaagg aatcaatttt atctccagtt ctgtatgact ttgttgctgc tgatggcgtg
    11161 cagcatactg tcagaaaaat taaagataac ctgttggctg caaaaataaa agatgcactg
    11221 cagcctcttg tcctgtatat tgctgatgga catcatcgtg ctgcatcagc tgtcagagtg
    11281 ggattggagc gcagaaaaag tggattttca ggaggtgagg catttaatta ttttgtggca
    11341 acaatatttc cacattcaga attacaaata ttaccatata accgtgtagt caaagattta
    11401 aacaatcatg acctcaggtc atttatgaat gaactttccc aaagtttttt agtagaaaaa
    11461 agtgatagtg gtattgttgc aaagcctcat gaaataagaa tgtattgtga tggacaatgg
    11521 tatgtgctgc actataaaaa agaattgccc ctgaatgatg ttgagcggct tgatgttgca
    11581 attttgcaaa atgttatatt ggcacccatt cttggcattc aggatccacg aagagattca
    11641 aggatagatt ttattggtgg cgtaaatgct gttaatgatc tcattgccct ggttaacagt
    11701 ggaaaattta aaatagggtt ttcattgtat ccaacttcaa ttaatgagct tattgctatt
    11761 tcggataaag gaggaattat gcctcccaag tcaacatggt ttgaacctaa gctaagagat
    11821 gggttgcttg tccatgttat tgggtaaatg ttttggagac gaggggagtc gaacccctga
    11881 cctcttgaat gccattcaag cgctctccca actgagctac gcccccggtt gtggttaaaa
    11941 tatgaatatt tatgttctta gtaatcaatc acaatatttt gtcaatattt tttggcatga
    12001 tatataataa attttataat tacactggat ttattcttgc aaaaatgata atcattatag
    12061 tagtataata ttatctgtta ctttaaggat ataccattct ggggttaccg tatgggaatt
    12121 gaaattgaaa aactgaaaaa agaaaatctg agtttaactg aagagatagc cgaattacgg
    12181 aaaatcattc agacatatga aaatgtcttg aagcttaatg agcaggaact atcgaacgca
    12241 taccagatta tagctatgta tgagaatatt gttgaatatt cacgctctga gttaaagctt
    12301 gcaactgaaa cagcacaggc acgggaaaat gcctcccagc ttagtagatt ggaattaatg
    12361 caagcatttg aaaagataaa acagcttgaa gaagaaaaca aaaggctcag agctcaaaaa
    12421 ggaaatgcat aaggtaaatg tataatccaa aaaaggcaac aggtagcaga gtatgctggt
    12481 aacatatgca ataatatttt tattcataat aattcttgtc atttttattc tttattttgc
    12541 aaatatttat atttttccac gtaaattaga agaaattcaa caactcattg aatcaggcca
    12601 gacgaaaata gcaatacgga agcttaatga tattatagaa aaagatgacc ggaatcatta
    12661 tgcgcattat cttctagcat tagcttatga aaaagaaaat aatatacagt atgctattct
    12721 tgaatacaga caggtattga aaatatcaaa atttgatgat aaggttgatg agatagttat
    12781 tcgaaaacgt ctggcaagca tatacaaagc gcgtaatgcc attgaagaag caaaaaaaga
    12841 atatctgctt ttaacaaaat tagaccctgc aaattatgag cctttttatg agttaggcgt
    12901 tatatttttc aatgctggtc aattagagaa agcagcacct tattttaaaa aaagtatttc
    12961 taataatagt agacatgctc catcatatta ttatttgggg caggttcagt atagaatgca
    13021 aaactatgct gatgctaaac agtcattcat tgaagcaatt aagattgatc ccaatgatta
    13081 taaatcacat tattttttag gcctggtgtt gcgacagtta ggtgattatg aatgggctat
    13141 taaagaattt gagacagctc aaaagagtga tgaaattaaa gttaagtgtt ttcttgcaaa
    13201 agggacctgt tttttagagc gtgaacagct tcctaaagct gtagtagaat ttgaacgggg
    13261 attgaaattt gcaaaaaggg gtagtgatac agaattaaat ttgcggtatt ttttagctga
    13321 tgctcaggaa aagatgcgtg atcttcattc ggctatatcg aattgggaaa aaattgtggc
    13381 tgttaatcca acctttcgtg atgttcagga aaaacttaaa gcgtatgctg agttcaggca
    13441 ggatgaccgc attaaggatt tcatgattgc tggccttgca caatttgagc atacgtgccg
    13501 taaaatggtg gaagcaatgg gtttgacagt tatggatgtt gatataatca gtgatactga
    13561 aatagaaata attgcaacgg atactgaagg caaatggcga aatacacgca gaactaataa
    13621 aattgtacgg attcttagaa caactgatgt ggtgaatgat aaattattgc gaaagctcca
    13681 cgaaaaacta aaagaaaaaa atgcaacacg cgttatcatt ataaccgcag gtgagtttac
    13741 tcagagtgca attgatttct caaatacacg tcccatagaa ttatatggaa aaagcgatct
    13801 tttacgttta ttaaaaaaaa cataactgta tgataaataa agatattctt atattaagac
    13861 aaactccatt ttttaaacat ttgatggatg aggagttgtc tgcattactt aaagtagtaa
    13921 aacctctggc tatccctaaa ggaggtatta taaactgtga ttcccattct ttatatattg
    13981 tcaaaaaagg tgtttttgaa attcaaaaac atgctgaatc tatttatata acaccacctt
    14041 ccttttttgg tgaattgccg ttttgtgaaa aaggttcagg gatagtaagg gcaataagtg
    14101 attcaattgc ctatgaacta aaagatactg acctgtatca gtttatgggc aataatttca
    14161 aggctttacg gggatatctt agaattgctg atttttatcg attttcttta acagatgcag
    14221 gaaaaaaatt aaacagaaat aaaactcaaa ttatcactgt tttcagtact gaaaaacatt
    14281 gtggcaaaac tacagtagca gtagcagcag ccactttgtc atcgtttaat aaaaaaacca
    14341 ttttgctgga tgcatcctat gatggcattt ctgtttttga tatgtgtggc aaacaattat
    14401 taccacctct atcccaaaaa agcccagatg aagtaactgg ccagaaattt attgagcaaa
    14461 gaattgaaaa agtatctgaa tcgttggata ttctcaacgt tgcttttggt tcaaaggtaa
    14521 gattaaatcc cgatattatc aatccactat tattctatct ttcttttgaa tattcctata
    14581 ttgttattga ttgctcaaat agtgatgttg ctctacgtga tactatattg cataatactg
    14641 atatcctgat tgaaattatt aataataaat ccatatccaa acttcaggca ataaatgatt
    14701 cattgttaac tcaggggcag caggttatat atattgttaa caaacatttt gaaaaaacat
    14761 ctaaaattga tgattcccat aaagattttg aaaccatttc tattgagttt ggtgaaacag
    14821 tttttcaggc aatcttaaga acaaaatctc aacatgatat ccttttccat gaaattttgt
    14881 ctgataaaaa agccctggtt tttgcatccg ggatgtatga aacactgtac tatgcaggta
    14941 tttttgaaac actgagggat tttgacaata tccctgatat aatttataca gccgggattc
    15001 cctatgtagt attgattgca tttttacatt caagttcatt gaaagaatat aaaaaattac
    15061 tattgagtta tttttctgat gataaattgc attcaatgct cgatgttaca tttcctgata
    15121 aatatttatt taaaaatgat cctttaaaat ctttttcgtc ttcagtatgt aatgctcagc
    15181 gacttgaata ttacaatatt attccgtata caatactttt ttctaataat tcaccgagaa
    15241 tgtttactac cggggatata cgtgatgttg ttgcagcttc ttttgctttc ctgccgttgt
    15301 ttgaaggagt gagtataaac aaggaagagt atcattcatc atttccatta tatcccgcca
    15361 tgcctgaaac gttattgcgt acacatatac agcaaatatc gtttataagc aacaatacaa
    15421 tgttcaatcc actttcatta aaaagtaaag ttttagggat ttttagaaaa tttgttgatt
    15481 ttcttccata ttactattta tatgcttgcg ataccggggc ggtttgtaat aaagagttct
    15541 ctttacagat accagaggtg acgtcaattg atgatcttat tgaaaaaaca tttgataata
    15601 aagaaaattt tgatgatatc atgataaaaa tagtaggggg gtaaatgaga tcaaaagcaa
    15661 caaacatatt acaatttgga gttttattaa caggaattat atatgttact attggacttt
    15721 tatatggatt cagtccaatt ttgtttgcaa atatttttgg aattgaggtt aaccctgatt
    15781 ggtataattt aattaaatat gatactttta cttcaccgtt atatcatttt tcacgagtat
    15841 ttgcattatt gatggcagtt gctggattaa gcatgattct tcctttattt gacccgttaa
    15901 aatatcgtgg aatgatatac tataatggta ttctttttcc tgtagtagca gcgcctgttc
    15961 ttcttgtcaa tggtcttacc tacgatcatc ttatattgac aatatgtggc gtgttgttgc
    16021 ttgtattatt tttctttgtt ggatttggat taatgataac tagaagacag gctaagatgg
    16081 ggcaagagta gtattttatt ataatttgct tgcttttttc caatttataa tgcaaaatat
    16141 tacacaaatt ttgtagtaac cttttaaaag aacttaattg tattggtgtt tgagtactgc
    16201 tcaatatgtt taaaatagtt ttttatttta caaaagaata aagccagttt taattttata
    16261 atcaggaggg tatgatgaat cttcaaacga agaaagttgg caatgttgtt gtagtatatt
    16321 tgcaggggcg tttagatgta catctttcag ctgatattga gaaagaaata aataaattaa
    16381 ttaaagacga gcccaatgct catctattat taaatctttc aggagttgaa tatatgagca
    16441 gtagtggatt gaggatattt gtctccacta tgcggatttt gaaagaatca aatagaaaac
    16501 taaagctttg taatatgaat agtgctgtta aaaagatatt tgaagttgtt gagcttatgg
    16561 acatgttcga aatatatgat actgaagaag aagccattgc ttcttttaaa taatagttat
    16621 gttgtttcct ttgattaaga ctgaggatcc tgaaaaaagt tctcttaaag acttattatc
    16681 tggaaataca gaaaaaaacc ctctatacat accttcggag atatccgaag gtatgaagaa
    16741 ttttataatt tatcaaaaat ataattattt tgtatggcct tattggatat ctcgatacgt
    16801 taatacagtt aatgagcgaa tacaatcact cccctttttt ctctataata atcattgccg
    16861 aacctggtat accatcaata gctttttaag cagcgggtca ataagaattg acccttcggg
    16921 tatggtccat ccagtatggt catcatggtc tttagagttc tggatatata cagataattc
    16981 tttaatacaa tttcataatg ataattatat ccagcaggga atagacaacg gtgtggtaaa
    17041 aacttcttat atccatcctg attttacctt taaacaatca gtttatatta ctcatacaaa
    17101 tatcgatgaa gcggttatcg ttaataatgt tcattataaa aagaaaaata aggcgtattt
    17161 tattatagct gtacggccat atacaactga aagattatca ggcctggaat cagttgaact
    17221 ggtgaaaaat aattttgtac gaataaataa cgaatatcat tgtgctttgc tgatgaagcc
    17281 cgattatgta cttacgggca atgaaatttc cggggacatt gatattagaa ggaaagaaaa
    17341 taaacataag gttaccagta atgcacgcat ggctacaatg gcttttgttt atgatataag
    17401 taataaagaa tctgaaatag ctatacgggt tgcggtaaat aatgagtcta aaatttcagt
    17461 tcctaaaata atcattaaat caacgcaaaa tgaatttaca agctatattg acctcagaat
    17521 gaaggaaggg gtggggataa cagttgggaa gaatagtata aatgaatggg taaatttttg
    17581 caaattttca gcgctggtag aagctgcaaa tattgatata agcgatacaa atcaaaacga
    17641 ggatattgcc cgtcattatt atacaataat gacattgaat agaatgggcc attttcaaca
    17701 gtcactatcg atcattaatc atatcttaaa aaatataaaa attaaagatg attttgaaag
    17761 tatactatct gctgcatatg tatgtaatat gctgggtgat tattacagga ttgttaagga
    17821 tacatctttt ttgcagaaca attatccggt ttgcaagcag tacgcacaaa atattttaca
    17881 attttccaaa gaattgaaaa acataaagaa atatattctg cttgaccatt ctgatgaaaa
    17941 tgcaatccac caaatatgga atattcccgt aataatttca gctctggaaa atttagcatt
    18001 tatgtcaaga tcattggggc tatttgggga tgaaacaaaa tttttagctg aagccaataa
    18061 acttgaggcg tctgtggtaa atgttttatt caatgtatct caggaaacaa gcaaagaaga
    18121 agaaaaactt attgaatggc tgccaaagca tggcaaacca ttacgaaagc aaaagatatc
    18181 agttaaagag agtctggaat ttgcaaatac tattaataca tccaataaca gaattctact
    18241 gtatacatcg tttttatatc cacatatagt tcatattatt cgaaaatata cagtacaaag
    18301 gattatagat gatattatta atgcaggcaa aggtttgcca ttgtataata caattattag
    18361 tggtatcgat gtcatcagtt ctcttaacct tgccaatagc atgttatatg ctcatgataa
    18421 gcgagctatt gatttaatat atgctattat aacgttatgt aaaaaacgaa aattcatgcc
    18481 tgatttcctt gatcctgtat ctggccacgc tattgtaaag gaacctatat caatagttgc
    18541 agtatcatta ttgtttcagg ctatccgcaa tatatgcttt attgatcatc cagaacgatt
    18601 agaattattc ccggttcctg ttgaggattg gtttggagat tcacaaattg taataacaaa
    18661 tgctccttca cgatttggca atataagttt tgtaacaact tctacttcca gtgagtttgt
    18721 tatacagttt actgatgttc cccgttttgt tccccatgat gttttaataa atcttccgtt
    18781 taaagcaaaa ataaagaaag ccgatgattt cattgttaaa tatgaaacag aaaaatcatg
    18841 gtgcattaat ggctggcctt cagaaatcag gtttaaacga tagttccagt tataacgata
    18901 atagagtagg tggtggttct taataatttt tagagtggga aaaaaatgat tacgtatatt
    18961 ctggtatctt tgtttataat aagtgctttt ctgtactacc ttgtctatat acgaccaaaa
    19021 tttagccctt tatataaagc ccagcaactg gttgagcaga atcagcttct tgatgctata
    19081 cttgaatata aaaaagtgat tgatgaatat cctgatgatt ttgttgtcca ctatcgcctg
    19141 gctgacctgt atttagctgt tggagagatt gaccaggcag ttattcattt tgaaaaggta
    19201 cttgaaattg gaaaatttaa ttatgaagtt gaccagcttt cagtacagaa gaaattggct
    19261 aaatcatatt atatgcggga tgagattgaa aaagcattcc agacgtatat ggacattctt
    19321 aaaaattatc ctgccgatat tgaatccctt taccatattg cgtttatctg tcttggtcag
    19381 gaagagtttg atattgcaca gcgttatttt gacagactgg taaagcttaa gaatgacaat
    19441 tatgaaattc tttttggagc aggaatctgt tattaccaga atatgcgtat acaggatgct
    19501 atcgataatt ttaaattagc agtgcagata aaacctcaat cagaaattgc tcagataggt
    19561 gctgcatttg catgtaatcg caaacgtgat tatcgccagg ctctgggata tatttccagg
    19621 attgcagata cagcccagga ccctgatgta ctgtatctgg caaaacgatt tcaggcattt
    19681 atgttgatgc gcctgaaaaa gcatgatgaa ggaataaagt tgtttcagga tttactggat
    19741 attgcacgga agaatgcaaa tgatgatgca gaacttctgg cattatatga tctggggtat
    19801 gcatatttaa agaatgaaca gacttcttta gcatatgaag catggaatga actttaccgc
    19861 agagatagaa actataaaaa tatacaatat cttgtaacac aattgcgcag ggaaatggag
    19921 gttgataaat tcagcaaaga agaatcggta tccgttacag aaactatcac aacatggatg
    19981 gatgatgctt tcccacaaaa tttattatgg gaaatgtgtg gtttaaaaag tacaaaaaaa
    20041 tataatttaa aagatatcgt tgttaccact aaaataagtt atgatgatgg aaaaacagtt
    20101 agtgtttctg atgatgttat ggacaggtat gttgctttag atactgagaa tttcagaatt
    20161 atttccaaca gactggtatc aaaaatgggt tttaaggttg atcagatatt acaaacatat
    20221 cgtgaagcag atggtgttga tttcctggca attaatcctg aaacaaagga taaagtttta
    20281 gtgtgggtca ggcggtggga taaaacaaat gtaggtgaaa taacattacg aaattttgca
    20341 caggccatta atgatatgaa agtgcaacag ggtatattta ttacaactgc taaccttact
    20401 caggcagcta aagaaagctt aaaaaaactc cccaaagtta cagtcattta tcctgaagag
    20461 attacaaaat tattacgagg gcttatataa tgatctgccc tgaaagtaat gttgtatcaa
    20521 taccattgga tgcaattcaa tgtgataata attatttcaa aatttcaaag ccgttgtcca
    20581 gggaactggt cagatcagta ttgcactatg gcattattga gcctgtgctg ctcataaaat
    20641 ggcaggagca atacgtgcct gtatgtgggc ataacaggat tatagcagcg cagcaagcag
    20701 gcataaaaga cgtcccggca aagattgtca ctgaattgag catggatgat tttttacaga
    20761 tagtaagagt taaattgtat aacaatgcat tagggaccat tgggaaaatt aagagtttat
    20821 atttactgta tgattatttt aatgctgata aaaaaaaggt aatggcaata gcacaggaac
    20881 tatcgctgcc tgcttcatta cctgaattta agaatataag atcaatcatt gaattgcctc
    20941 cggtcatcat tcagtatatt gattccagat caatttctcc aaaatttgtc atgccaatat
    21001 tacggttacc ccaaagctca atagctctac tggaaaaaat tacttctgca tgtcagctca
    21061 gggtaaatta ttttaacagc atggtagtga tgattgagga tatttataga atggggaagg
    21121 ataaacttct tggagatagt atagaatttc tgctaacaaa agaaactata ttagatgatg
    21181 atatccataa ttgtatatat tcaattcgtt acccacagta tacagaatat atggttcgta
    21241 ttaatgagat aaagaaaaaa cttaacgcct gtggtattaa agtggatata ccagcatatt
    21301 tggaggggga tagtattaac gttatctttg aggtaaaaaa gaataagcca ttgcatccat
    21361 tgctaaaaaa agatcaggat attttagggt tgattgagtt gcttataggg ttgttataaa
    21421 tgtaataggt tgtttcatga aaaactgcta atttatttaa aaaaacgtga catttcttct
    21481 aatactttca atcgtgtagt ttcaaaatca tacaatgatt taaagcctgc agcattttta
    21541 tgtcctccac ctccatttgc aatagcaatt tcggcaacat ccaaatcgcc ttttgtgcgc
    21601 atgctgactt ttactggacc tccaatgtcc tgttttatca gaacacttgc cacaacaccc
    21661 tgaattgata agggtaaatt aatattaact tccccttctg aaaatggtgc attggtttct
    21721 ttcagcatat caggggttag cttcatcagt ataagtttcc caccataatg gacttccatt
    21781 gtggaaagca tcattgctcg caatctgaat acttccagtg aattattttc ataaatctgc
    21841 tgataaattt catatggctt tacacccagc gttaccaatt gcgcagccaa tacaaatgtt
    21901 acgggtgaag ttttagggta cttgaaacaa ccggtatcaa aaactattcc ggcataaaga
    21961 gcctgtgcag ctttaaatga tgggattact ccaaaatgct gtaaaatgtt gccaattatt
    22021 tctgatgttg atgaagcttc aactttaatg aagtttgatt taattttgtc tgtttcccct
    22081 tcatggtgat ctataataaa tacatcctgg attgaatctt ttaaataatg atatgctttc
    22141 ccaatattat cgaagtcatt tgtatccaga acaaaaacag caaaattatt tatattgtct
    22201 atcttcatgt ttttattgaa tattattatt tcgttatcag gatcaataaa actgaatttt
    22261 gatggtgttg ggtcagaatt tataattatt gcctgcttcc caattttttg cagtagttct
    22321 ttaaatgcaa tctctgcacc aagcccatca gggtcaggac tttcatgagt ggagataata
    22381 aaattgtcat gggcatcgag atatttttta agattagcaa agtaatccat caaacgttct
    22441 cggattgagc atatttacaa agtggatacc ccattgattc gcgaattgca atatacctct
    22501 cagcacaacg gcgtgctaaa tttctgaccc gtgcaatata tccaacacgc tcagttactg
    22561 aaatggcacc gcgtgcatct aagatattaa acacatggga acatttaagt acataatcgt
    22621 atgcgggaag aaccacctga actggttcat aatcaagtat cttaaggcat tccctttcat
    22681 aaaggtcaaa tagtttaaaa tgtaaatcaa tgtcagctat attgaaattg taatgagaaa
    22741 attcaaattc actacgatga tgtacatcgc gatatgttaa tgtttcattc cattgaatat
    22801 caaatacact ttcaacaccc tggatataca tacaaatgcg ttcaaggccg taggtaattt
    22861 ccacaggtat tgggtctaat tcaacgctgc cacactgctg gaaataggta aattgtgtaa
    22921 tttccatgcc atcaagccat acttcccagc ctaatcctga agctcctaac gtaggtgatt
    22981 cccaatcatc ttcaacaaaa cgtacatcat gctcctccag tttaattcct aaattacgta
    23041 atgaatcaag ataaagttcc tggatgttga gtggggaagg cttcataatt acctggtatt
    23101 gataataatg ctgtaatcta ttaggattgt caccatatcg cccatcggtt ggtcggcgtg
    23161 atggttcaac gtatgcaaca ttccatggtt caggccctaa aacacgcaga aacgttgcgg
    23221 gattaaatgt accagcacca acttccagat catacccctg tgcaattata catccctgtt
    23281 tagcccaata gctattaaga ttagcaataa gatcttgaaa atacatgata tgctccctgt
    23341 aaaaataagc gttattttgc tatctgttga tttgttttaa tatattcgtc gtatctggct
    23401 tttgtcaatt catgaagctg ttcaaagtca tgtttattgt tcagcttaaa atagtcaaga
    23461 cagtcttcat cggcaatttc aaatctgaaa ttttttgttt caatactgct gaaaacatat
    23521 gctaaatata caatattgat taaatcccgg tattggggcg gggataagtg aggcctgtgg
    23581 tgaaattcta ttgctttgac taatgcatcg ttaaatttcc atttctggca aatcatgctt
    23641 cccagtgtac tatggctaac tccaagagat atttcttcca gtaatgagat gttttccagt
    23701 tgttttaaac caacgatatc acgtatcttc tggatgtcat caggtttaat ggaaagaagt
    23761 accatcaggc ctatgtcagc cagtaaggat gccaggtaag caaattcgga tagcctttcc
    23821 ttacgatatt tcattgcaat gaattgtgca taaaaagcag ttttatatga attattccat
    23881 attgtttcaa attttttgta acgggaatcc agtattttct gaacaccact tgcaacaagc
    23941 agagtgttta tccctttagt gcctataatt tttactgctt cttctatggt ttcaactcgc
    24001 tttgatgtaa tgtagcctgc agaattggct aattttaata ttgcagtaac aagcccgggg
    24061 tcacgtttga tgctatctgc tattgcttta atagttgatt cggggtttgc acataacctt
    24121 tgtatttcaa gtatattttg aggaaatgat ggcaatgatt caacctcgtt gattatttcc
    24181 tgtgccatct ttactttcag gtccatattc cggaagcttt catcgatatt tatagaggca
    24241 ctggtaactt tgccatttgt ggatattgca aaagcttcag gtggaaaacc tgcattcttc
    24301 ataagcatga gtgcaataat taaacccagc cctgcacctt ccgaatcatc aagtatatca
    24361 tcaaaagcat ctgaaatatc tgaatagctg taagcctttt ttacccgtgc atttatttta
    24421 ttcatttcct cgggtataat tggaacatta ttaattacat gtattttgtg agtatccccc
    24481 gtaattgaaa aaaagactcg tacaatgaaa tttgattgac caagctgttc aagaaattca
    24541 gggttttcga caaagacgtc ctccttgaaa gtttccatgc ctgtgcggta gtcttcggtt
    24601 ttgtttatgt ccaaattttt ttgtttaaaa tatagccttt ttgcatttgc ctttattgca
    24661 ttattaacta attccttaat aatattaata aacatatctt ttaaataaag aaggtcaagc
    24721 tgagcaaggt aaacactgag cattcgtatg atgatagatt ctacttcggg tgttaaaagc
    24781 gagaattgaa tataaacatc ctggcccatt ttaagggctt ctttatagtt agctggctta
    24841 caaaaatatt gagctttttg ttgcaatcct tccaatgttt tctccccagg gtaacaatat
    24901 ggtaaaacta tacatatcta caataatatt gcaagaaaat taatacacca tgtcaagaat
    24961 ttattctaca gtaactatcg gctattgtaa tcttttgagt aattacttaa aagagctatt
    25021 aataggtaat agaaaagtat tattgacaat tttgctaaat ctataaataa taactgtaaa
    25081 caaaaaagag gtgggtatgg caaaagtata ttttattgat atgcatatga gtagccgcga
    25141 aaatattagt agcaaaatta aaaaattgtt ctataaactg gaagttaact caattattaa
    25201 tgaaaaagat tttgtagcaa taaaaaccca ttttggtgaa tatggcaata ttgcatattt
    25261 gccacctgcc atcatacgag cttttgtgga ttgtgttaaa gatttaaagg caaatccttt
    25321 tattacggat gccaatactt tgtatcgcgg ttcacggagc aatgcaatag atcatttaaa
    25381 aaatgctgag atgaacggtt tttctatatt ttcgtgtggg gcacctgtta taattgccga
    25441 tggtttgcgt gggaatgatt ttaaagaaat ccctgcttcc ggtaaatggt attcgagtgt
    25501 aaaagtggca tctgctattc atgatgctga tgctatgata gtggtaagcc atgtaaaagg
    25561 acatgaattg tatggttttg gcggggcaat aaaaaacata gctatgggct gtgcaccacc
    25621 ttccatgaaa cagtcattgc attcagactt aaaacccagg gttaaacagg cattatgtac
    25681 atcctgtggt gcatgcataa agagatgtcc tgcatttgct atttcatttg atgacaataa
    25741 aaaagcattt attgatagta ataaatgttt tgggtgtggt gaatgtactg ttatctgtat
    25801 ttataatgca ataccggtag tatggaaaac agatgcaact gctttatttg aacgtacagc
    25861 agagtatgta aacgcgttta tgaaactaaa gaaaaataag gttgcatttt tcaattttat
    25921 tatgaatgtg tctcccgaat gtgattgttg ctactggaat gatacgccta ttgttccaaa
    25981 tataggcata ctggcgtcaa ctgaccctat tgctatcgac agggcatcag tagacctgat
    26041 aaataaagct atgcctttgc ctggatcccg tattgctggt aaagatgcat ccaaagataa
    26101 tattactgcg ctttaccctg tgcagtggga atatttattc aagtatgcaa cgtcccttgg
    26161 ggctggtgat gaggaatatg atttaattga aatttaggca attttagcag cttttgttaa
    26221 gtttgtaatg aagaggtaat gatttatgaa gagtccacat gattttcctg caattaatcc
    26281 aaaaaaacct gatggcataa agcctaatgg tataccatat aaggtgatgg tggttgaaga
    26341 taaagaattt catcgtaagc agattattca aattttagaa tctgaaggat atgaggttat
    26401 agctgctgct actaatggtg ctgaagcatt aaaattatat gattcacttg cagctgatct
    26461 tgaccttatt acaactgatc tggatatgcc aaagcttgat ggttatgctt tactgtatga
    26521 gttgagtcaa aagaaaccca aggcaaaaat tgtatttatc agcgatgaaa ctaccaaagg
    26581 tgttcttgct gatttattga aaatgggtgc tgctgatttt atattgaagc caatccagag
    26641 agggactatt ctggaacgtg ttaaagcagt gttgcaaaaa aaataatata ttatccataa
    26701 gggtttaatc tcatggaaga aaatgagaag gcatatcagc atctttcgga acaaacaaaa
    26761 aagataaata tagcactgca ttatactcat ggagatatgg aaaaggcccg tcaaatggta
    26821 gcgggcaact ataatgatct atatgccatt aaagtacgat ttgcttcttc taccatgttt
    26881 ggtgcatttt tgcttttttt taatcatata tataaaatta tttcaggtct gacgcttatt
    26941 ctatcgccgt catatacgtt acaaaatatt aatccggaat gggactggat agtttttgaa
    27001 aaagagatag cagagctaat gaagaaaggt gactatgacc agattttagc taaaaccttt
    27061 cgtgacaaaa taataaaaag ccttgatatt gtactgttaa atgagttagc ccgcaatatt
    27121 gaattaaaaa atgagatagc aattgataac ctttttagaa agctcataca ggaatcactg
    27181 gcattaaaaa gggttgattc atttgttgtt tatcagccaa cgtcatctct tactatggag
    27241 ttacattcag ttacaattcc taaattaaaa agtgaaattg ttgatggtga acaagtaaca
    27301 aaatcagaaa cacagccagt cgcaactggc gaaactgagt atacaccaca ggttggtgta
    27361 gatggggtta agcttatcat taaatcagct ttaatcttat cacctattaa gggcaaacac
    27421 atctcaaaac tgtcacctgg cgatagagtc atgatatcca ttattgatac caatccacag
    27481 gcggtaaaag tggcacaggc tttaaacgct tatgaaaatg aaacatttaa accaattcct
    27541 gcccgtatca cgtatgttaa atacattcct caaaagggct ataaagtata tggtttaatc
    27601 gctaaaggta ttgttgttga tatcgatgag gaagaagaaa acattaaagt agcgcttgac
    27661 cctgcttatg ctgctaccat tcagtccccg gaagaagagg taaaacctaa ttatgtgctc
    27721 atagccattc ttttgctggc atttggtatt ttaatgggta ttctgtattt gttgctatgg
    27781 tcaaaataga ttagacagga cgtaccggaa taccgcgcat ggctaaatac tcttttgcct
    27841 gcttaatgct gtattctctg tagtgaaata ttgaggcggc taacactgca tcggcttttc
    27901 caattgcaaa tccctcatat aaatgtttta gggtaccaac accgcctgag gctatcacgg
    27961 gaatggtaac tgattcagct acttttttag taagttcaag atcatagcca agtttagttc
    28021 catcacggtc catgctggta agtaaaattt caccagcacc taaatcttca acttgctttg
    28081 cccattgtac tgcatcaata tcagttttag ttcttccacc gtgaagatat acattccatc
    28141 cgccgttatt gcgtttagcg tcaattgcaa ccacaatgca ttgtgaacca aaacgccgtg
    28201 cacattcggt gataagctct ggttgctgta ctgcctgtgt attgatagac accttgtcgg
    28261 caccattttc cagaatcatt cttacatcat caacagttct gatgccacct ccaacagtaa
    28321 aagggatatt cactgtttct gcaacttcct ttaccatctt taaaataatg gcgcgcctat
    28381 cgcttgatgc ggtaatgtca agaaatacca gttcatcggc tccttcttca tcataaaaca
    28441 tgccgttgtg aacgggatca cctgcatcct gcaggtttac aaaatttaca cctttgacta
    28501 cacgcccatc tttaacgtcc aggcagggga taattcgttt ggctaacatt ctacggtatc
    28561 ctgaaaaata aattgtacag ttatatggta tagatgcaat tggtatatgt caaatagtta
    28621 aaactggttt gacaatcaca actcaatcta tcaaatggaa ttgttatgtc aaaaaaaaat
    28681 gaatacatat catttgctga attttcgcgc acctacgtgc ctgccatcaa tgagtacata
    28741 tatacgtttt attccaataa ggaactatcg gcacataatc cagatataaa actggcttac
    28801 aatttgctta aagaatattg tacacgtgat ggcaagcgca tacgcccgct tttattatta
    28861 ttgtcatacc tgggttatgg tggccctatc tccgaaatac aatcagttat tccagtggca
    28921 gcctcacttg agtgcatgca cgcattttta ttaatccagg atgacatcat tgatggagcc
    28981 ataatgcgcc gtggcaaacc agcatttcat aaggtgtgcg atagttctta taataagtac
    29041 aacgaacata ttggttcatg ggtggcaata gtactggctg atgttctcat gagcaacgca
    29101 ctggagatga tttccggatt aaccacaacg tctatgtctg cgtttttgca tgaatttgcg
    29161 ataatatatg aacgaacagc actggggcag atgcttgata ttatatggtc taatccaaaa
    29221 tatatcgatg atgaaaatat cccacacgca attgcctcaa tgaaaacagc acattataca
    29281 gtttcaggcc ccttgcggct gggctacata cttgccgggg gtagcaacaa tgatgagttg
    29341 caaaaattgg agcaggctgg ttataaagta ggctttgcat tccagatgcg tgacgatatt
    29401 attggtgtat ttggcgttga ggaggatagc ggtaaatcag ccagttcgga tattgaagaa
    29461 ggtaaatata ctgtgctggt gcaaaagacc ttgcagcttt tacatgacag tactgttaaa
    29521 gagcagttta tggccttatt taaaaagaag caaaaaagta aagaggatat tcaggccatt
    29581 aaagcatata tagaaaaaag tggtgccaaa gcaaatgttc agcaactgat ttcccggtat
    29641 gttaacaacg caaaagaaat tataccacag ttacaatgtg attcatttat gaagcgttgc
    29701 ttgttggatt ttattgaaaa tttgtttaat tgaaaccttt aagataataa aaataattga
    29761 caaatttaat taatcatatt aaatttgtcc atatgatata taaaaatcgc cagctggaat
    29821 acattctaaa agaagctagt accacatttc ctgcattact ccttactggt ccacgacaat
    29881 cagggaaaac aacgctgctt aaaaatttat tttcagataa agcaaaattt atatcattag
    29941 atgaatccga catacgttta tgggcacatg aagatccccg agattttatt caaaacaata
    30001 tgccacctgt aatcattgat gaaattcaat atgctcccga tttgcttacc tatataaaac
    30061 gaagaattga tgatgaccga tcccctgggc agtggttctt aacaggttcc agccaattca
    30121 cactgatgaa gcatgtttca gaatcgttag ctggccgaac ggctattttt catctgctac
    30181 ctttttcgca ttcagaaata catggtagag tatatcccaa ttgggatgat tggtttacgt
    30241 caattaaaga taatgtcccc tgtgatgaag atttaggtaa tgtgttactc agaggaacat
    30301 atcctgagtt agtatgtaat cctaccataa atcgtagaat atggtatgat tcttatattc
    30361 agacatatat tgaaagagat ttatccctga tttataatat tggggatatt aatacgttca
    30421 ctaaaatgct tacacttctt gcagcaagaa caggaacaat tcttaacatg tcatcgttag
    30481 ccagcgattt aggagtatca gtgcccactg taaaacgctg gatatcagta ctggaatcaa
    30541 gttttatcct atacctgtta aaaccgtatt atgctaatat aggaaaaagg cttattaaat
    30601 cagcaaagat atatttttta gataccggac ttgctgcaca tttaactgga attagggata
    30661 gggaagtctt attgaggggt ccactatcag gtcagttatt tgaaacgttt gttgtttctg
    30721 aatttgtaaa aagtttttat catcatggtg aaaggccagc gctaacattt attaataata
    30781 aaaatatatg ggaagtggat cttcttatag agcgcaatat gaacatgata cctgtagaaa
    30841 ttaaactttc agcaactatt accgaaaacc atttgaagaa cttcaagttg cttcgcaatg
    30901 tactgcaggg tcttgatgac agtaactatc tcatatgtaa caggaaagaa tataaccgca
    30961 tgaagggtac tcatatatca tactggggat cgttgtagtt tctataaaga atgtaatatc
    31021 ccacactaac atgattccaa tgataatcaa aatcatcagt gttttggaag ttatccagaa
    31081 tattgtataa atatactatt ttgattataa taattttctt gccaaaatgg ataaaataat
    31141 ttactttgta aaattggttg acgaataaat taattattaa gcattgtgct gtaaacaaaa
    31201 gctgtcaacg gagcagataa atggtaccaa caacggtaaa aaaacatcat aaagatacat
    31261 ttgaaaaaat ttcgcctgaa aaacgtgaac gtattataaa aattgccaca caggaatttg
    31321 caaaaaatgg atttaaaggg gcaaatatca atattattgc caggaaagca ggtgtcagca
    31381 ttggttcaat gtataattat tttgaaagta aagaggatct tttacttact gtagtcaatg
    31441 aggcatataa gctgcttgaa aaggttttgg gatctgttag ccttgaaaat aaaaatttct
    31501 atgaaaagat agaggaactg gtcagagcag cccaaaaatt tgcacgcgaa taccctgaaa
    31561 tacatcaggt ttaccttgat ataacatcag aaggcctgtc acaccttaca ccacgtttgt
    31621 caaaaaccat tgaatatatt accattgaat tttaccgcca tgagttggaa gcagccaaac
    31681 gtgaaggcat tgtgcgtcag gatgtggata ttaacgttgc ttcactgtgt atcgataata
    31741 ttctggtgtt gactcaattt tcatatacat ctgaatattt taaagaacga ttgaaacttt
    31801 ttattggtca ggatgcttta cgtgatgatg aacgaattgt gaacggtgtt atggatttta
    31861 ttaaaaatgc tttgatgcca aaataaaaaa aaatatgcaa gaatgaatta ctattcatac
    31921 attgtagaaa ggttaagtgc cgatgaaaaa gaaaaatgtt aacatcattg aagtacgcag
    31981 gaatcagttg accagagcag cgtataaggt agtcagccga aaaggttatt ataattttac
    32041 tgttcgtgat attgccagag aggcagggct gtccacaggt ctggtccatt attattttaa
    32101 aaacaaggat gacctgctgg tatcagtatt gcgtgtcatg aatgaaaatt taagctttta
    32161 tttagcaaag gccctggaac aaacgaatga tccaaaggaa aaaataatta tttttatgga
    32221 tgaagctttt catttagttg agcgtgaaaa agaatatttt catgtattaa ttgatttctg
    32281 gacacagatt aatcataatg aacgaatgcg aaaagccaat gttaaactat atcaaagtta
    32341 ccgtgctgag tgttccaaaa tacttcagga aggtattgaa aaaaaagtat ttaaggatgt
    32401 tgatgtggaa tacactgcaa caatgatagt ggcgtttgta cagggtctca tcatacagta
    32461 tattattgat cccaatgcat ttgattatgc agagtataat aaaaaaataa aacagcagat
    32521 aattgaaaat cttatacgat agtacagagt tttaatcttg attaacaggt gtatatgtga
    32581 tataattgct acctgcaagg tattattaat tttctggttt acagtatgtt taaagatata
    32641 tctgaaaacg gcgattatca aaagcattta gagcatgttg tacagacatt atgccaacct
    32701 gagtcatata caaaagtata tcaccgaacc aatcatgacg ttcccatgcc ttccatacca
    32761 caactggcgg aaattgttga agaatgtaag ctggtactgt ttcctggtta ttttggcaat
    32821 tctgagataa ccccccaaaa catgcaatat tatataggtt cagctttaga tagtattgca
    32881 cgcaaactaa cagagcagat aaaacgcggg tactgtttct catgtgcaga aggtgaaacg
    32941 atggattgca ttgagtgtga gcatcgttca cggaaaatca tgtttcagtt tttagatatg
    33001 ctaccagaga ttcgtcggat gcttgccctg gatgtacagg cagcctatga aggtgacccc
    33061 gctgccaaaa gccatggaga aaccatattc tgctatccaa gcataatggc aatgattaat
    33121 tacaggattg cccatgctct gtatattctg gatgtgccac tcattccgcg tatcatcacc
    33181 gaaatggcac attcacagac ggggattgat atacaccctg gagccaccat tggtgaaaaa
    33241 tttttcatag accatggaac aggtgttgtc ataggtgaaa ccaccattat tggcaacaat
    33301 gtccgcatct atcagggtgt aacattgggt gcaaaaagct tcccgcttga tgaaaacggc
    33361 aaccctatta agggaatacc gcgccaccca attgttgaag acgatgttat catatattca
    33421 ggtgcaacca tattaggcag agtaaccatt ggcaaggggt cagttatagg tggaaatgta
    33481 tggttaactg aaagcgttcc accggggact catgtgctgc agcaaaagcc aatggagatg
    33541 ctttttgaaa atggtggtgg tatataacaa tacattttct aaaacagtaa tcttagttac
    33601 aaaatgggag atgcttttgt aatagtgccg tttatgatgt tttgctgcct atcaataatg
    33661 ctatgtgttc tttccccttt gaccagtgcc agttattgcg agcattgaaa tacaaatacc
    33721 aatggttttt atcaacctgt tttaaatcaa atgcatagac atggcttcgt ttatatgaac
    33781 cttctttgat atcaggtttc aacaacgggt ttttcattaa cgaggtgtag tgtataccat
    33841 cttttgatga gtatactaaa atggctgaac ccgactgatt tatagtataa tcccagtaaa
    33901 tcccgttctg caacccaatg aaaccatcat ccattttcag ggctttaaat gaaccgcacc
    33961 caatattgca ccatcggtta tagtgtgatg gctccatgat ggggtgggga aggctttgaa
    34021 aaggtcctgc aggatttgaa gattgtgcca gtccaatata cagtggttca ttaaatccac
    34081 aatcctggat gtgggttaat gcagcactgt aatatagaaa atatgattgc ttagttttaa
    34141 ctacgcatgg attgcttaca gacgccccaa atgtattact atgccaggga agtgatggtt
    34201 ttaaaattat ttgttcatca ctccatgaat ataaatcatg tgaagaacgc attgcaatat
    34261 atgaatacca tgtatgtgga agccatgaat accataagat gtacggttta atcttttcgt
    34321 aatataaata gtacgtgtta tcttcataat aaagaaaagc acgcatagca ttgccaatgg
    34381 tgtgctttga catcctggtg taattgatgc cgtcatctga aacaaaatgg tgtatcccca
    34441 tcagggaatg tgcaaataaa tgccatttgc catcaggtga ctcgttaggt ggtacaaacg
    34501 ttggatcggc aattatcggc gatgggaagg gtggggcaat aatgggattg ccttcgtatt
    34561 ctttaaacgt aaggtgcaaa aaatctttta ttttcatagc atgctctttt ttatgtgggc
    34621 gatagttcat tataagtagc tatcgccaca aaataattta ctgcctttaa cactacctgc
    34681 taattgtaca tactatacat atactacgtc aagccttatt gttgacattt tgaaaataaa
    34741 gtttttgact ttttgttgtt tatatgtttt atgttcatta caatagtgtg ctgtatgaaa
    34801 ccgatagtta cgataataat tctgctatat gctgcaatag ctttaactgc ctgtactcct
    34861 tcagtggaga ttgtacaatc acttgagggc acctggcagt tgacaactac caacaaagat
    34921 gagtactgta gtacagtatc tatcgataga atcccgatta cagtgccatt tgttataagc
    34981 cttccccata ctctggaaga caaaaagaca ggaaataaaa ttacattagc tgaaagcggc
    35041 tatgtgtgga ttcagaaaga gtttgtgctg gatacagtac ctgcgcagga cgtggcactt
    35101 caaattggtg agataatgaa tgctgatagg gcatattgca atggagtctt tataggtgca
    35161 agcggcaggt tcccaccaca ttttaaatca gcatggaacc agttcaggca ttatacaata
    35221 cctgctacgc tgttgcacaa aggcaaaaac cttttatcca taaaaatata ctatgatgct
    35281 gaattctgga taaatgctcc tctgcggatg gttagcgata gttccggtaa aacggctttt
    35341 gattttatta actttatgcg tgtacatctt atgcaggcaa tgttttttat catgctgaca
    35401 ctggctatat tctttattgt gatgttttat tatcaaaggg attcggtgga atatctattc
    35461 tttgggcttg cgtgtttatc ggtttcattc cctcagcttc ttcattttat tgaaaactta
    35521 tatcctgatg tgccgtattc atccaatact gtgctaaagg tttcacagcc agggttgata
    35581 tttttcccgg cattgttaaa cctgttttat cggtatcatg ttggaaggaa taagctctgg
    35641 ataacttcaa tctatctttt aattcctgta gtacttacag tggtattgat ttttcagaag
    35701 acacgcagtg atattttatt tatacgaaac ctttcaattg ttgtatcaat tttgtttatg
    35761 atagacatat ttgtaacctc aggcttgcaa ttaatgcaac ataaaaaggc cggtttgctt
    35821 atctttattg gtatagtccc tatggtcata ttaggactgc atgacgtact tgcttttggt
    35881 tttaagatta ttccttcgga tgttgtatta tatgtgtatg gcctgccttt tttattattt
    35941 atcattgcaa tttaccttgt aaatattttt attacagcat tgcaacaatc aaaagaattg
    36001 aatgtccagc tacagcaatt gttgcaggat gcaagaaaat tagcatatct ggaaaaagag
    36061 cttgaaattg ctcgtactat acaaaatcaa ttacttccat ctgaaatccc ccatcttaag
    36121 gggatggata tagcaatacg gtttcaacct gccttacgta ttggtggaga catttataat
    36181 atttttcagc ttaaagatgg cattgtcata ttccttgctg atgtggtggg gcatggtatt
    36241 ccagcggcta tgatagcctc catggtgaat attcttttta agatgtttca gatgttgtct
    36301 gttgatcccg ggttgctttt gcgaagcttg aatattaata tatgcaatat gctaccgcaa
    36361 acctttgtta cagcggcatg tttgtatatc aatcgctatg aaaaatttgt gcgcattgca
    36421 cgtgctggcc atccatcaat tattattcag aaagctgatg gtaacattct agaatatatg
    36481 cctcagggta aagcgttagg attgaagact gataataaat acgaaacgtt aaatcaagca
    36541 gtatcaccgg gtgataagat tttaatatac actgacggta tagttgaagc tatagatatt
    36601 aataaaaata tgtttggctc tgaacgattg attaacattg tccagcaaac aaaagaagca
    36661 tcatgtgctg aatcaattga ggctatctat tctgaactca tacactttgt tggtgatacc
    36721 tcagcacttg aagatgatgt aacgctgatt atgataannn nnnnnnnnnn nnnnnnnnnn
    36781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36841 nnnnnnnnnn nnnnnnnagc tcaaagaaat atttatcaaa ccaagaaaaa atattttttt
    36901 attgacaatg atggtatata ttttgattat ccaaaaagtt agataaaact aacaatatta
    36961 ggagataaaa actatgctaa agaatatagg gtgtcttttg gtatttgtta tattcctatc
    37021 ccctgctgat gtatatccat atagaccttt tataacagaa gatgcaggtg tgagtggatt
    37081 aggcgaacag aaagttgaga tggggtttgt tgggagcagg caaaatgaat actggtacaa
    37141 ttatatatcg ttgttgtatg gaattggttt tggtaaagct gaaatacttg ttgaaacacc
    37201 ttactgtata aatgatgagg caagaggatt ggaggggatt atcctggctg caaaaattaa
    37261 tgtttttgaa gttgatgata cattgatgtt aggtgctaaa gctggatatg aatttcctga
    37321 tgattgttat ggtgtaagtg caattgcaac aaaaaaaatt ggcatattgg taatgcattc
    37381 ccaaataggc ttaaaaaggg cttttaatga tactgttggt ttatttgggt taggttttga
    37441 atatcaattg ttgcaacacc tcagtgtaat aattgataat tttacagaat atgatggtga
    37501 tgctttgtat catagagtgt taacgggagt aatatatagc tttaatagtg aaatagcttt
    37561 agatgttgca acaggatatt gttatgcaaa caatcacaac aattacagag aatattttac
    37621 tgtagcggga atatctttta tattttaaca aaaaatattt agcttgattt cccatgttat
    37681 atcatttata catcatagca attctttttc gtgcttgagt gagggaataa aaagatgaaa
    37741 aaaatactca tcataattag tgtatccatt ataagcattt ttacgtgtta taatgcatca
    37801 tatgcacaaa cacattttcc agttcaacaa cttattgata atgcattgca atcttttgaa
    37861 ttgcttaaag ctcagcaggt aaaaattgat gagcttgagg cactaaaaaa gtataacacc
    37921 caatggaaaa atcctgaaat agctgtttat tatggcagta aatccattga ttctcagaag
    37981 ggtcatgttt caggtattgc tgttacacaa cctctttatt ttcctggcaa actttcactt
    38041 atagatgaaa tatatcgtta taaaaagaat tatgaagtac tgtcgtatga agagatgaaa
    38101 tattttatag caacaagtgt cattaattta agctatgagt atgcaataag ccagctacgg
    38161 tcttctcata ttaattcaag aataaagcgt ttaaatttat taaatactta catgactgcc
    38221 aggccattgg tttcgcctca aaaaaaggtt gaggtagcaa ttgtaaaaaa tagtatacga
    38281 atgcttcaat cagagattta tcggataaaa gctgatcagg cagttaccta tgcacgatta
    38341 gccttatata cacaattgga ttcacctgat ttgaatattt ctaccaaatg gattgaaaat
    38401 cccccagtat ttgatgtaaa tgatgtaatt gcaagagcaa aaaatgaaag tattatggta
    38461 aaaaaacaaa aagaaatact aatggcttca ttaaaagaaa tttcactggc aaaaaagaat
    38521 atgtatcctg attttaatat actatttaat tatgattatg aaaaagtata tgaaaaagaa
    38581 aagtctattt caggaggcat cagtattcct attccacttt ataatcaaaa tcaaaatgct
    38641 gttgatgctt ttcagtcaaa agcaaaacag gaagaattat acttacagta tattgaaaag
    38701 atgattgaaa gtgatatccg cacaacagta gcacagtata attattacct caatttatta
    38761 ccattatttc ctccatcaat agaaaatgaa ctggaacaat caatgtatta tgctgatgat
    38821 cagtttcaga aagggacaat aacgttgcaa tcgtatttag agctggatgt acagattcat
    38881 caaacattag agaacatata tacaacacaa aaagaacttg tgcgttctat ggcagccata
    38941 gcaactttta taaatgatta taatctatta ctggagatag caaaatgata tcggcctgga
    39001 tagaaaaagc actaactgga aggctatgga ttttaatcgt tacaattggt atagtcattg
    39061 cagggtttta ttcaattggg aaattaccta ttgatgcagt gccagatatt accaatgttt
    39121 cagtaatggt taacacaaaa acgggggctt tagctcctga agaggttgaa agaacagtaa
    39181 cattccctat tgaatcagaa ttggcgggat tacctgatgt tcaggatata cgttcactat
    39241 ctaaatatgg cttatcacaa gtaatagtag tattttctga taatactgac atctattttg
    39301 ctcggcagct tgtcaatgaa cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39421 nngttagaac gtatggcaag atagattcac tgcaacagat taaaaatata ccggtaaaat
    39481 tgaatgtata cggagcccct gttcgattat ggcaggtggc agatgttgtt gtaggacatc
    39541 aacagcgttt aggagcagcc acactcaatg gtaatgaaac agtgttaggc actgtgttga
    39601 tgcgtattaa tgctaacagc agggaagtat cgttagctgt tgaaaaattt ataaaggaaa
    39661 taccattacc atctgatgtt gaaattaaaa ttttatatac gcgaagtttt cttgtaaatg
    39721 caactattaa aaccgtttcc cgtaatttaa ctgagggagg tttgttagtt ataacaattc
    39781 tattttttat cttaggtaat ataaaagctg cattcttagt tgctcttgca ataccattgt
    39841 caatgctttt tgctactagt ggtatggtag catcgggtat tgctgcaagc ctgatgagtt
    39901 taggtgctat agattttggc ctcattgttg acggttcggt tgtaatgatt gaaaatatag
    39961 tgcgccattt taatacagtc aaaaataggt cattaacaaa aaaagaaaag attgttataa
    40021 taggtgatgc cgcaaaagaa gtgagccagc ctgttttctt aggagtctta ataattatgc
    40081 tggtgtatgt gccagtgtta agtctcacag gtattgaagg caaaatgttc agacctatgg
    40141 ccgaaacggt attgtatgca cttggtggca gtatcataat agctttgttt cttatgccag
    40201 ttctggctta ttatagtttg agccctaaaa agacagataa agagccattc gttttcaggg
    40261 ttattgaaaa ggtgtataat ccttttttaa cattttcttt aaagcatcct gtgaaggtta
    40321 ttatcccaac attgttggta gctcttatct ctattattat gtttatacat ttgggatctg
    40381 attttattcc caggcttgat gaaggtgata tggttattgg gcttgttcgt gatcagacaa
    40441 taagccttga ttcttcactt gagatacaga aaaaaagtga ccaggtaata agccagttta
    40501 aagaggtcaa aaccgtattt tcgcgtatgg gaacgcctga atcggctact gacccaatgg
    40561 gagttaattt tgctgataca tttgtcattc tggaaaagga caaatcaaag tggggattat
    40621 acaatggaaa acacccaacg aaagttgagc tatttgaggc gatagctcta aagatcgatg
    40681 aacaggtgcc aggacaggaa atttcactca ctcagccaat tgaaatgcgt ttcaacgaga
    40741 tactggaagg caaccgtgca gatattaccc tgcgaatcat tggccaaaat ctggatattt
    40801 tatatgaact atcgaataaa tcaattccta ttcttgaaaa aattgaggga gcttctacgg
    40861 tagaacttga tcctttaaca gcattgaaaa aaagcgaaat tattgatgta gttctcaatt
    40921 atgatgctat atcgaaatat ggcgttaaca ttaatgatgt aaataatgct ttttcaattg
    40981 caatgagtgg taaacaggtg ggttatcttt atgatgaaga taaacgcttc ccaattatga
    41041 taagaataag cgacgttttt cgtaacaacg ttgcaaatat tgcaaaaata ccagttggtc
    41101 tggctgatgg aggagcgata ccgattggta ctgtagcttc agtgcaaaga caccagcaga
    41161 taacgacaat agcgcgaaca cgagcacaac gatatgcagc agtttcaata aatatcaaaa
    41221 atagggatac acagagtttt gtaaatgaag caaaggaaaa aataaaagcg ttaaacatcc
    41281 ctaaagggta tcaactacag tggggaggcc aatttaaaaa tttagaatca gcacgtttac
    41341 ggttacttat aataattcct atagttttgg ctattatatt tttaatatta atacggacat
    41401 ttaaaagttt aacacagacc ttacttgtat ttaatagcat accttttgct cttacgggtg
    41461 gaattatgtt gatatatatc cgtaatattc cgctcagtgt ttctgcagga ataggtttta
    41521 ttgcacttat tggtatagca atactcaatg gaatggtgct tgttaatttt tttaatcagt
    41581 tacgcggtca gggtgtaaag ttagaaaaag ctgtgtatga aggcacatta attcgtttaa
    41641 aaccagtttt gatgacagcc cttgttgcta gttttggctt tattcccatg gcttttaaca
    41701 caggcatagg agctgaggtt cagcgccctc ttgcaacagt ggttatagga gggttgattt
    41761 cttcaacaat tctgacgctc ttgttattgc caacgctgta tttgtgggtg gagaagctgt
    41821 ttggcaagga ataagaaact ctgtgtgttg caaaaatgac acggtgtagc ataaatagta
    41881 aacttcattg taactccaga aatagataaa taatagaaaa ctctcgaaat tccagcactt
    41941 tcaaatgttt cccggaaaag aaaagttggc acaaattttg ctaatttata tgattagaaa
    42001 agctaaggag cataaccatg atgaatatac caattataca ggtttttgtg cttttctggg
    42061 tatgtgtact tctttgggtc attattgaat atattgttta tagaagtcag aaaagaggtc
    42121 attaatgtag catatccggc aggatacaat ccgtttattc ataacaatca atcccagttt
    42181 ttttatttta ctgcggcttt ttagctgcag ttttttttgc acgatagtta ctaatagtga
    42241 tagacattac agagaaatta aatactatgt attaagttaa caagcctggt tgaacaagaa
    42301 ctataacacc tgcagtcaaa ggtcagaaaa tctggaatta aatggattag aacatggcag
    42361 tagaaacagt tggataataa ttttatgtta gattgtaaca aatctggaac acatgagatg
    42421 gctgagtggt caatgcattc tattttcaag ggagatgtta tgaaaaagtt actaccagta
    42481 ctgatagtat tatttacaac aatagcaaat tcacaaacct atctttatga gctatcggac
    42541 aacattgaaa catataaaaa taagcaaatc acaatgatat taaaactaaa acggtatgat
    42601 acggtatttc aggtcataac attttatgat aacaaaaatc atgatattac atttgactgt
    42661 attccattgg ttaaacaacc atggtttata caacaaagat taaaccttca tcctggaatg
    42721 gaatatactg taacatttac cgttaagggt gtgggtaatc ttaaagaagt gattgctgac
    42781 cttaactact ttgaacctgt aattcttcaa aagctccctt aatactataa taaaatacta
    42841 taataaatgt atttgtctta aaaaacgaaa ggcaagatag ctgggcggct atcctgcctt
    42901 cccctaaatg ggtaaacgta tgcgcagtgc cttgagcaca aaaagtaatg atcgggaggg
    42961 atttacttat gtgctcctgt cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    43021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    43081 nnaaaaggtt tgcttttcaa tactcaacaa aaatattata gaaaggagta tttgtatgaa
    43141 aaagatgtcc ttgttttttg cagtgatgct ggtaatgatg atggttgctt gcagcagcac
    43201 tccggaagtt cagcagccag caggtgatcc caatattgct ttggtaaatg cacagaatca
    43261 acagcttgaa aaaataccgg taaccggctt tgcttataaa agtgcagttg tgcccaaaca
    43321 gagctgggat aaatgggcag aagtagcagc tccagtagta aaggaaattt taaacaaatt
    43381 gcctgaaggc tatgtattgc aggtaaccgg tcatgctgat ggttcaggac ctgaagaacc
    43441 ggttggcaat aaaccaggca acatcaaaat atccactgaa agagcaaaag ctgtttataa
    43501 tgctctcaaa gctaaaggaa tagattcacc caaaatgact tacaaaggcg ttggttcatc
    43561 tgaacttctg ccaggtgtgg atccaaggga tgctgcaaac agacgcgtta cttttaaagt
    43621 tgttccaaag aagtagtaca gacaataatt taatgttggt tttgatgtat acgtgtagag
    43681 ccagttttat gactggctct acgttattgt gattaaggtt tacctggtat gcatggtgta
    43741 gtttctaaag taatggggct ttattatacc gtatatcatg attcgacata tacaaactgc
    43801 gtactcagag gtaaattgcg ccagaagttt gaatgtgata ctgaaaagtt ttccaatcca
    43861 gttgctgttg gcgatgaggt tgaaataacc atagctgatg atggtacagg tgttattgag
    43921 gagatagttc caagaaaaaa ttatttttca cgcaaagaga aaggtaaaca cagaaaaata
    43981 gatatcattg ccgcaaattg tgattgtgtt gttgtaatgc aatcatttgc actgccaaaa
    44041 ctaaatttgc gatttgctga caggatagca gtcaggtgtg gtcagcaaca tattccactt
    44101 attctgtgtg ttaataaata tgatttagct tcaaaaaaag atattgaata catatacaat
    44161 tattataaaa atactgatat cagtattgta atgacaagtg ccaaaacagg ctacaatatt
    44221 gatgagttta aaaacctatt atacaataaa acatcagtta ttgttggcta ttcaggagtt
    44281 ggcaaaacta gcatattgaa taccatctat ccacatctgg atcttagagt aggtgaggtt
    44341 tcagcaagca ctggtaaggg taaacacacc acaactaatg taaccatgat aatagtggaa
    44401 gagcacacac ggattattga tacccctgga ctgcgtgagt ttgggattat ggatattgaa
    44461 cctcatctgg taagtaaata tttttatgaa tttgaaaaat atgctgcccg atgcgctttt
    44521 aagccatgca cccatgacca tgaaccggat tgcgaagtga agcggttggt gcaaaaagga
    44581 gtcatatacg aagaacggta tatttcatat attaatattc tctattcctt aaaagattat
    44641 tatgcaacga tgtactcatg ataactgcag aacatgttcg gttaacattt aatcatcatc
    44701 ccgtgttgca cgatgtgtgc ttttctgtac cacagggctt gatctatgtc attcttggga
    44761 aaaatggcag tgggaagaca gtgctgttaa aaacaattgc agggctattt actcatttta
    44821 caggaactat cgccttagct ggcattccgt tacaggaata catcaatctt actatagaag
    44881 aaaaaatgaa acgcaatata accatggcgt atgtgttcca gaaaggtggg ctttttgatt
    44941 ccatgaatgt gtttgataat gttgcttttg gattgcggcg gcgtaatgaa aatgaggcga
    45001 tagttactga aaaagtcatg gaagcattag aatgggtggg tttgtcagga tcagaacaaa
    45061 aactaccctc ccagttaagt ggtggtatgc aaaaaagagt gggccttgca cgagcgttat
    45121 gcctgtcccc ttctataatt ctgtatgatg atccaaccgc agggcttgac ccggttcttg
    45181 ctgattcaat ttctgatctt attgttgcca ctcaggaaaa atacaggatg acatctttac
    45241 tggtaacgca ggatttagct gttgctcaaa agacagctca ccgggttgga ttgctgcata
    45301 atggcatgat tgttgttgat ctggaatctc atgaattttt ttcaaagaat aatccatatg
    45361 caaagcagtt tattgatgga gatagcgaag gaccaataga tagtatgtaa aatattaaca
    45421 taaccttaat aatatttcaa ttaaaatata tttgacactg tactattatg ttggtaaaat
    45481 aatataaaca tgtttaagct atttaaccat attaaaggct atggttttta ttttttttta
    45541 tgagggttta tgagctatgc aaacacaacg agaaaaatct aagttagact ggcaaaatct
    45601 accatttgga tacataaaaa cagattataa tatcagattt tattataaag atgggaagtg
    45661 gtctgaagga gagctttcaa ccgaagaaac tatacctatt catatagctg cacctggcct
    45721 tcattatgga cagcaggcgt ttgaagggct taaagtcttt gaaactgtgg atggtcgaat
    45781 tgtagctttc agacccgatg agaatgcgaa acgaatgcag cgcagttgtg aaaggatttt
    45841 cattcctgaa gtgcctgttg atatgttcat taaagctgtt gatacagtag tagcagcaaa
    45901 tgcaaaattt gtgccaccat ttgggacagg tgctagcctg tatgtgcggc cagttatata
    45961 cggtgctgga gcaagagttg ggttgggacc ggcggatgaa tatgtattta ttgtttttgt
    46021 gactcctgtt gggccttact ataaaggcgg ctttaagccg gttaaggctc ttgttgttga
    46081 acaatatgac agagcagctc ctaacggggt gggtgactgc aaggttggtg gtaattatgc
    46141 tgcaggattg cgcggtggtg aatatgccaa aaagaaaggt ttcccaatac cgctgtatct
    46201 ggatccaaaa gaaaagaaat atgttgatga atttggtacg tcaaatttta tagcaatcaa
    46261 gggcaatacc tatctaaccc ctgaatcaaa ttccattttg cccagcataa cgaacaaaag
    46321 ccttatggat atagctaccc ttttgggtat gaatgttgaa aaacgcccaa tagcaataga
    46381 tgaagttgag cattttgatg aggtgggcgc tgttggaact gcagcggtta ttacaccagt
    46441 atgcagcatc cactatcgtg ataaagtgtt cacattttgt gatgaggaaa aagcaggacc
    46501 tactattacc aaattatatg agtacctaac gaaattgcaa actggtgaaa taaacgatac
    46561 atttggctgg cttcatgaga ttacagtaaa atagattaca tcacaatcag gcaattttat
    46621 atatgacaaa tcaggatata cgatggatac aacgttttaa tcattttatg aatgctttta
    46681 aacgattgga agatgctgtt acattgtata atgataggac actatctgat ttagaagagc
    46741 agggtttaat acaggcattt gaatataccc atgaattagc atggaatgtt tttaaagatt
    46801 tccttgaaga cagaggtgtt actaatttat atggttcaaa ggatacgaca cgttatgctt
    46861 ttaaaaatgg tttaattgat aatggtgaag tatggatgga catgataatc agccgtaatt
    46921 taacttctca tacttatgat gaagaattag ctaaagaaat tataaagaaa attgtgaatg
    46981 tatatataaa ggaatttgat aaaatgtttt tgaaatttaa tacgttgaaa aatgaaaatt
    47041 aaaatatgaa atttggatta tcagaaaaaa caattgattt gatttgctct atttttagac
    47101 aatatcctca gatagaaaaa gttattgtgt atggttcgcg tgctaaaggc aattataaaa
    47161 atggctcaga tattgatctt gcgctcattg gaggagatga tttaacaatg gatgttttgt
    47221 ataaaatatt acatgaatta gatgatttgt tattgcctta ttcttttgat atttccttat
    47281 ataaatttat acatgaccct gatctaattg atcatatnnn nnnnnnnnnn nnnnnnnnnn
    47341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    47401 nnnnnnnnnn nnnnnnncac catgaagcag gcaaaaactt tgatgaactg gtagtaaaaa
    47461 tttataattt ttctctcatt gtgcgtaagg caattaaaaa gaaaagtgtt ttatttttac
    47521 ccacagccaa accgtttcaa catgatgaaa ccgatatatc tgtagtaatt attcctctat
    47581 tgcatgcaga tattgttgtt ggcatggtat gtctggtttt tcctttagaa gaacttcaat
    47641 acttgcagaa caatgcacca tttttcatgc ttattggtaa aatcacaggt actatacggt
    47701 attatcaaac aggccttaag gtcagcaaat ctgatactgc tgaggaagaa tttgaaaaaa
    47761 ctatagccag tttacgggtg cttgctcagg gtgtagcaca tgaatttaat aatattctgg
    47821 cggttataaa aggttatgct gaacttttgc tgatgaatga agctgtggat gaatcagtca
    47881 agaaagcagt gtccattatt gataaacaaa ctaccagagg ttcatctctc attgacaggc
    47941 tgagtgtatt tgttactggt aaagaaatct catttgaata ttgttccctt aatgatttga
    48001 ttaaagagat agttgatttg caaagattta cgttagaaaa agaatccatt gagtgtaaat
    48061 taaaattggg caaggttccc gaaatatttg tagactcacg ccagataaag gaagtacttc
    48121 tgaacctcat tacacatgcc cgttcggcaa taaagcccaa taaaaaggga actatcacca
    48181 ttcaaacatc ttttaaccgt gatgcggtta tgcttaaact ggttaacgat ggtgttcccg
    48241 tcaactggga aaatggtggg gaacagaaag caaaaggtag taatggtgtg gatagtgtgg
    48301 gaaatggcat taatttggca attgcgtatg gcataatgca aagccatggt ggtgatatga
    48361 cactgtgtca tgatcccgcc agatgtaatg aagtaacact ctattttaaa cgttttaatc
    48421 ctgcgtttga tgctgttgga aaaaaagaag acaaagagat gttgtattta ggtgaagtga
    48481 gaattttaat agtggatgat gaagagccta tacgccagtt tttatcttct gcttttgaac
    48541 gaatgggcta tcaggtacta acaactgata atggacagga agccattgaa atatgcacat
    48601 ttgaggatat tgatatcgtg tttttagatt atttaatgcc cggatacaaa ggtgatgcgg
    48661 ttttcaagtc aatacatgaa gttagccctc aaacggatgt tgtatttatc acaggtgtcg
    48721 attcaatacc tgatattgat aagctattga tgatgggact ggaaggcgtc cttagaaaac
    48781 cctttaagat agaaacaatc atcaaattaa ccaacgaaat aatggcacga cgatacaata
    48841 cattacagga ttgaatctta ttctgatttt attactcgtt cagcctgaag ggctaaatat
    48901 tttaccaggg ttggttcatc gataaatcct aaattcataa gtattatacc tataagccca
    48961 ccttcctttt tctggatgga taaagcctga ttcaattgat cctgggttat aactccatct
    49021 tcaactaaca gttcacctaa caatggctta gccataatgc ctccttgcaa tagaataatt
    49081 aataattata ttagtacatc tgatatttgt ccagtattat aataaccgct ttactgcatc
    49141 tttccacaga gctatttcac gtttccgttc agtctcattc attgatgggt tgaaccgttt
    49201 tgctatcttg ttgaattttg caacctcatc aatggtattg tatacttttt cagttatacc
    49261 agctaaatat gcagcaccta aggctgttga ttcagtaact tctggcagca gtaccggtat
    49321 acccaatata tcagcctgat actgcattaa aaatgtatca cgtgttgctc cgccatctac
    49381 tctgagttca tggatattct tgcctgagtc attctgcatt gcttctaaaa cttcgtatga
    49441 ttgcaaagca attgatttta ctgcagcacg aacaatctgc tggggtgtag tatcacgggt
    49501 aagcccaaaa atagcgcctc gtgcatccat cttccagtat ggtgcaccca gaccaacaaa
    49561 tgccggtact accacaactt catcctcttt tttaaccgaa agcgatactt tttcagcatc
    49621 agaacttttt tcaataaatt tcataaagtc tcgaagccac tgtactacag ctccaccaat
    49681 aaatactgaa ccctcaaatg catacacagg ttggccttta ttgtcgcatg tcaatgtggt
    49741 aagcagtcca tgctttgaaa gcatccattc atttcccatg ttgagaagca taaagcaacc
    49801 agtaccgtac gtattttttg atgttccctc aaaaacgcag ttctgaccaa ccattgctgc
    49861 ctgctggtcg ccggcaatcc caccaatggt tatgccatca ggtaatcctg gaacaccact
    49921 tgtttcgcca aaatatgatg ctgattccct cacttcaggc agtatggtgc gcggtatatt
    49981 cagtatttct aaaatatcca gatcccaaat tttctctttt atattgaaca tcagtgtacg
    50041 ggaagcatta gtgtaatcgg ttttatgaga tttgccgcca gttaacttcc acaaaatcca
    50101 ggtgtcgata gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    50161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccaggttt
    50221 tggaaaatgc tgtgtaaatt cagcatagct gcttgaaatt ggcagtccat gttcgtcaaa
    50281 acagaatacg cggttaccag tagtacctaa atcaatggca ataaggaact ttttacccat
    50341 aatacaaccc ctccattatt tttatgtgcg atagttactg tacattaaag ctacataata
    50401 gctgaaaata gtatggtaaa atgtgtttaa ggctttacag tgaagtcttt agaattttaa
    50461 taccgttgag cgcttatctt actgttttca ttaataattt gtcaattaaa ttttgaggaa
    50521 gaaaatgagt gatatataaa aatatcttgc aaaggtatgg cttctttgaa acactgtgta
    50581 gcgtttatta cgtttttatc atttgtaaaa aagcaatgtc aggagatcag gaattatgaa
    50641 tatagttaag aatatgatac tattgcaagg gaatagtata tggatttatt tgaaaaaaat
    50701 tatctgaata agctgcaaaa taacgaacca gtatttattg cagaaattgg gcttaaccac
    50761 aatggcaaat tggatgaagc cattgcttta attcaaaaag cagcacaagc aggtgctgat
    50821 ttagttaagt ttcaagtttt taatgcagaa aaattttatt cagtgtatac aaaatcgctt
    50881 cttaaccatg aaaaaccgaa gtatgatgat tgcagcatac agttttttaa aaagtttgaa
    50941 ctttcattta acgattacag gacgctccag cacgttgctt atgatcatgg tgttgttttt
    51001 tttgcatcat gttttgatga tgattctttt gcaatgatgg aaaaccttaa tgttccattg
    51061 tacaaaatag cttcgtcaga gattaccaat gtgccattgt tacagaacat tgtagccact
    51121 cataaacctg tgattgtttc tacaggcatt tcattgcaac atgagattgc tcatgcaata
    51181 caccttttgc aaaaagaaca gtgcaatgtt gtgctgttgc actgtgtatc actgtatcca
    51241 gtaccacatg atgcaattaa tctgttgcga atcaaaaggt tacaaaaatt atatggtcta
    51301 ccagttggat tttcagatca tacaaaggat aatcgtgctt ccatgatggc tgttgcaatg
    51361 ggtgcacggg tatttgaaaa gcattttaaa ttatcgcaca accatgattg tgcagataag
    51421 gatgtatctc ttacaccaga agagtttaca gcctacattg aagatattaa tatggctctg
    51481 gcaatgcttg gtgatggatc aatcgattat aaagaaggtg aagcagccgt tgcccgttcg
    51541 gcacggcgaa gcatttttgc agcacgggat atacctgcag ggacaagcct taactacagt
    51601 gatatcatcg tcaaacgacc gggcgtgggc ctatcgccgc tacatagtga tgctattata
    51661 gggaaaaaag ttaaacatac aatacacaaa gacatgccta tttatggtga ggatcttgaa
    51721 tgagtgttga tcttttccat cgctttttgc aatggctttt acaattaatc caccacgatg
    51781 caagcgtggc ttatttcttt ttaaaactat tgaagaagca aatgaattta gattaaacga
    51841 tcagattgaa aaagtcttaa aattgtttta ggagtatact cactaaacta taaaataaaa
    51901 ttgttgaata aaaagagtta taacgaaaca ttgtgtagtg attgttttct atatactata
    51961 aaactgcaag gtgagggaat ctcagaatga aaaagatagc cattgtattt ctttttttta
    52021 ttgtagtaac tccagcatat gcatcaacca ttgattatat attcaatgat aaccaacggt
    52081 taattttgaa ttccgctacc gacataatcc aggcttcatt agggtatgat gatatcaggg
    52141 agttagtgta tataacattc tggagcactc agcccgctga tgccaaaaaa attgatacat
    52201 tcaatatgta tattgctcag caatacaaaa ccagccctga tgatgttatt tttatttatg
    52261 agcgtttatt gcagtcagtg tacatgattg aatacatggc tgatctggca aagtcgaaaa
    52321 agaaatggaa gttctattac tactattcag atacactgtt acctgatacc aggcggtttt
    52381 gtgatacttt aaaacaggct attataaaag cagatccatc tattatagaa catataaata
    52441 aacgcgatgt aagaataaaa agctatgcaa ttgacatcgt tacatacaga gaagcattgt
    52501 atggaggagg gttttaaaaa aaagccccag aggggctaaa agttttgtta taggctgcat
    52561 tagcactctt ttattatgag tgctaacaat aatatacata aaattcatta tatcgtcaag
    52621 tatttttgtt atatttttta gagtattttg ctgtcaggat tataccctaa ctgcactgac
    52681 aattgaagtg aggtatctcg tacagcatgg ataatgtcct gtttgtgttt gagtataaaa
    52741 tcagcattac cggtaatggc aaatgctgca ataataccac tataattaaa gataggccca
    52801 ccaattccag ccaatcccat cactgactcc tgattatcaa aactaattcc agcctggcgc
    52861 acctttttta tttctttcat aaagtcttct tttgatgtta ttgaatttgg tgtcagcatc
    52921 tggaaagcat aatcttttag aatttcttct aatcgctctt tttccatata ggcacacagt
    52981 aacttgccta tacaggtggt atgtgtgggt ataactgaac cggcttgagg atatgacata
    53041 aacctgtttt caggctcaac gcggagtact atgactactt tatctcccac aagcatgcca
    53101 atattgaccg gttgcctgaa ttgaaagcat agacgttcgg cccataccct ggcaatgctc
    53161 accaggtcaa ggttggtggc atatttcatt cctaactgga atacatacgg accaagacgg
    53221 tatttgcctg tttcgtgatc tttttccaga tatcctaaat gacacagtgt ttgaactatt
    53281 ccctgtatgg tggttttggg taatgttaat tgtcgggcaa aatcgcttat gcccagcggt
    53341 tttttatcat taatgaacag agataaaata gcgtgtgcac gctcaattga ttgaatgcgt
    53401 ttaatttttt ttgtcttcat aattgtcata ctatataaca aaaagttatt gacagtttac
    53461 atattcaaat ttatattgaa tataatatcg tacaatgtac gatattatcg tacattgtca
    53521 actacttttt gtaggagtat agaatggata tactgaaaaa atttgagatt actgatccgt
    53581 atgagtatgt tgctaattta aaaaaggcaa caggcaagag tgtgcttgca tatatgtgtt
    53641 cgtacacacc ggaagagatc atctatgccg caggtattca cccattcaga atatttggtc
    53701 aggtggagac aatctctaag gcggatgcac atctgcaggc atacagctgt tcgctggtac
    53761 ggggtgcact ggaggatgca ctgcagggca ggctatcatt tgtggatggt gccgtgtttc
    53821 cgcacacctg tgattcaatt cagcggcttt ccgatatatg gcgcctgaat gctggcatga
    53881 agcatcattt tgatgtggta tgggctgtta agctcaatac tgatagtgca cgaaaatata
    53941 tgcgcgatgt actggtgcgc tttactaagg aactggaaca aagctttgga atatcactat
    54001 ctgacgataa gctgcaaaaa actattgcta cttataatga aatccgctcg ctgttaaaaa
    54061 ctatctggca gcttaaaagt gataacaaaa gtgctttaac tggcagccag atgaatgcag
    54121 tcatcaaagc agcaatggta ctggaccgtg atcagctggt ggtcaattta aaggaattaa
    54181 tcccatccat tgagctatcg ccgcaaactg aaaaacgaaa acgcataatg cttattggtg
    54241 gtatttgcaa tcatcctgat atttacgatt atattgaagc ttcgggtggt gttgtggtat
    54301 gggatgattt gtgtactggt tcgcggtatt ttgagggggc gatagcaact gataaggaac
    54361 cgttgcaggc aatagccgac aggtactatg aaagaaatat ctgcccggca aaacaccgtg
    54421 attattacac ccgtgctgaa accatcaaag caatgattaa aaagcataat gtgcagggtg
    54481 cgatattttt gtatctcaag ttttgcgatc ctcattcgtt tgactatcct tacctgaaag
    54541 aggctctgga tgaggttggt gtcccttcca tattgctgga actggaacag caacttccat
    54601 cggaagggca gatgaaaacg cgacttgaag catttattga tatgatttaa cttttgcgag
    54661 gtgaacctat ggcagaacca caggaaaaaa aggacaacag aataaaatcc gttaaacgga
    54721 tgaaagagat aatgaccaat tactatattg aggcaaaaac tgctgctcaa aacaataaaa
    54781 aggtagcgtg gatcaccagt ggagggccgg tagagcctct tttagtaatg gatgtaattc
    54841 cggtgtatcc ggaaaatcat ggcgcgatgg ttggtgcatc aaagatgggt gtgcagctat
    54901 gtgaaaaagc agaggaaatg ggctactgcc gtgacctgtg ctcgtatgct cgttcggaca
    54961 ttgctgctgc gcctatcaac ggtgggccta ttggtggatt gccaaagcct gacttccttg
    55021 tttgctgcaa taatatctgt ggcactgtgc tcaaatggta tgaggtgcag gcacggtatt
    55081 tcaatgtacc attgtttatc tttgatacgc cgttctgtca cagtgagatg actcaggaag
    55141 caaaggatta tgtgggtgca cagatacttg aatacattgc gtttttagag gaacactgcg
    55201 gcaaaaaatt tgattatgat aaagcaactg aggttggcaa actttcggtt gcaggtcaac
    55261 gattgtggca ggcagtgctt gatactgcaa tgcataagcc cgcaccaatg agtgcttttg
    55321 atgcattttt ccatttagca cttattgtga cattgcgcgg tacccagatt gttgtggatt
    55381 attacactga attgctcaac gaaatgaagg agcgtattgc aaatggtatc agtgcaattc
    55441 ccaatgaaca gtaccgcttg ctgtgggata atcttcctgt atggtataaa acaaagtggc
    55501 tttcggatac atttgctgca cataatgcat gtcttgtggc tgatacctat actactgcat
    55561 ggtgcaggca actggattac cttgatgaaa atgacttttt aaagagtatg gtggagggat
    55621 ataccgctat ctacctgaat atttcggtgg aacagatggc aaaagctgtt attggcatga
    55681 tagacaaata tgatgttgat ggccttgtga tgcattcaaa tcgcagttgt aagccttatt
    55741 cgttggggca gtatgatatt cagcgtattg tgcaaaagga gcgagggatc ccatcgctta
    55801 ttattgaagc tgatatggtg gatgagcgca atttcagtga agcgcaggta ttaacacgca
    55861 tagaagcctt tatggaaatt attaaaagta aaaaataata tatttttgga ggccttatat
    55921 gtaccaaaca aagccatggc ttaaatacta tggagatgtg ccacattcac tggattaccc
    55981 taaggtaacc atgtatgaag cgctgatgcg tacagtggag cgtaaaccca atgctattgc
    56041 ctgggacttt atggggtata cagccacgta caaagagttt gcacaactca ttgataggtg
    56101 tgctgaaggg ttatatgcat tagggcttaa agagggtgat accataacta tctcaatgcc
    56161 gacaacgcct caagggataa tttgctttta tgctgtcaat aagattgggg ctgtggccag
    56221 tatgatacat ccactatcgc ctcccaggga aatcgaattt tatcttacta taagcaacag
    56281 tacatttgcc cttaccattg atgcctttta tggtgctttt aaggaagtta tggataaaac
    56341 cccatgtaaa accttaatac ttactaaaat ttcagatttt ttgccagggc ttaaagcatt
    56401 ggggttttat ttaacgaaag ggaggaagat cccaaaagtg cccaaagatc cgcgtgtggt
    56461 ttggtggtct gacatgatga aaaagtcaag taaagttccc aaatccagca tgaaaactga
    56521 tgacatggca gttattttat acagtggtgg cactacaggt atgcccaaag gaattatgct
    56581 ttcaaatatg aattttatca gtgaaggcat gcaggttgct tcatggggaa aaatgtctga
    56641 agatgattca atactggcaa tattgcctat attccacgga tttgggctgg gtgtttgtac
    56701 caatgcattt tttatgggtg gtggaaaaag tattctggta ccacaatttg atccggttat
    56761 tgttgcaaaa cttattaaac aaaagaaacc caatttcctt attggtgtcc caacgttgtt
    56821 tgaggcattg agtaaaagca aggtgtttgg ttctgcagat ttatcatgta ttaaggcggc
    56881 tttcagtggt gcagataccc tgcctcgtgt tgtaaaggaa gaatttgaaa gggtagtagc
    56941 acggcagggt ggtaatgtaa aattgcgtga agggtatggt ctaaccgaag ctgtaacagc
    57001 aattatggca atgccaatga atgaatatcg tgaaggtagt attggcattc ctttccctga
    57061 tatgatggca aagattgtta aacagggcac aactgaggaa gctccatgtg gtgaggaagg
    57121 tgaaatatgt gttgctgggc ctgctgtaat gctgggttac cttaatcagc cagaagaaac
    57181 tgcaaaaacg ctgcgcaagc atgatgatgg tttggtatgg ttacataccg gtgatatagg
    57241 gacaatggat gaggatggat ttttctattt caaattacgg ttaaaaagaa tgatcaagtc
    57301 atcgggattt aatgtatacc catcacaggt tgaagacgta ttgcgtaaac atccagcagt
    57361 agaccaggcc tgtgtaatag gtgtaccgga taaagcacag gtagaaaggg taaaagcatt
    57421 tatagttttg aaaccggaat ataaggataa agcgggtagt gcattggcag aagaattaat
    57481 tggacattgc agggaacatc tgataaaatg gagttgccca cgggaaattg agttcagaga
    57541 tgaattgcca aagacaaaag ttggcaaaat tgcttttaat gtactggaag aagaggaaaa
    57601 ggaaaaatta cgaagccgtg gagaatatgc tggataaact tccgataata ggagtatgaa
    57661 cagactttct atatatattt ttgaatttgc atttcgattc ccgtggctgc ggtatattgt
    57721 ctgggctatt attgttagtg taagtatcta tgcaggttac catctctttt acgaagaggt
    57781 ggtaacctat ccagtgggat gggagcggag tgctgtcata acaccatata cactgaaagt
    57841 aaagtccccc tttgcagtgt caaagggcaa tgtcgttgca gttgtatttg agggcacgga
    57901 aaaaaatgag catggtattt atattatact ttcacttaat ggagggaagg attttctacc
    57961 accatataaa ttatcagata ttgaccctgc tgtagctgta cgccctcatg ttgccatatc
    58021 aaataacggt cacattgcct gtacctggca taaccttgtt gatagtaaaa gtaacatttt
    58081 cctgacaact tccaatgatc tgggtgccac atggacaaaa cctgtagccc tattgcttca
    58141 gacggatgaa tccatgttgt cacaggtgtt ttatgattcc acgggcgggc tgcacctttt
    58201 ttatcatggt tcagtaggtg gtcgtcttat tctgtatcat gcattttccc ccgatgaact
    58261 gcaatttaca aggatagata caatagcaga tattgcaacg gaatatagag gtatattctt
    58321 ccctgctata gtttttgaag gtgattcaat atatttagta tttcaggcaa agatattgtc
    58381 cggaggggta ttatctgatg acctgttctt tgtttattcg aataatgccg gaaaaagttt
    58441 ttccaatcca cggaggatta ccacaagccc ggctaatgat tcatctcctt caatactgtt
    58501 gcacaatgat gttatttact gtgtgtatca gaataatgaa aagaggcaat gggatgtacg
    58561 gcttttaaag gggtataaca ggggaaagga gtgggagcag caaccggtta tagtaactga
    58621 tataaacgtt gattgttaca accctcgcat cgttgcttct tcagatgatg aattgcttgt
    58681 agtgtggcat gatatgcgtg aacgctatcc tgcagtattt ggtaggaaag tatctcttat
    58741 tgataattca atgactgcag aagtgcgaat atctgatgca aatgtaacgt catatgatca
    58801 gtttcctgtt gttattaaaa agcaagttct gttgttctgg aatagcaacc agcgccttat
    58861 ggcaaagttt tctgatattt ctgtacttcc tccaaacgtt tattcacgga cacatccaga
    58921 aaatgtatgg acccgcaaca caaatgccat cattgaatgg gatccgccag ttgatgaatc
    58981 aggtatagct ggatatgctg ttatttataa tgaccttccc acatttgatc caacagtaca
    59041 gaacgtggaa gggccaatac gaaaatatgt tgttccagat ttaaaggatg gtataaccta
    59101 ttttcatatt cgcgctattg ataaagctgg caattacagc cgcaccatcc attatccgtt
    59161 gctggtttcc agtacgccac tacccatgcc cacagtgagc tctccaacac atcctgaagc
    59221 cacacaggtt gataatccag ttgcagtagt gcagtggaca attgaacaaa gggacagact
    59281 taaaggattt tactatgcta ttgccaaaga tgcaattgtt gaaccatcaa cattcacaac
    59341 tgctgattca gtgactcttg aaagccttcc aaaagggaga tattttttca gcattcgtcc
    59401 tgttgatatg accaacatga aaggtcggat ggcaacatat gagatagtta ttggggatgt
    59461 tggaaaagct gatgcggcat attatgaacg tttagccaaa ggtgttttac caccagaaga
    59521 gttaccagaa aaagtcccgg gaaaggtaag aatacagtat cccattgtgc tttttaatat
    59581 accgtttaaa gaaatttatg cgtcttcatt ttacaaattt tcattattac caaaaaatat
    59641 tttgccaaag caggttcagg ggtattcagt tgttgtttct tcacaaaaag tagtaccacc
    59701 ttttatcgtt acccacccaa ccagtggggt aatgcttacc aatgtaaaac ctggcagtta
    59761 caccataact gcccgtatgc ggtatgtgta ttaccagaat ggcaaagcag tatataaatg
    59821 gagtgcaccc agttatacca cgctggttgt taaatcagca tatacatctc cattaatgaa
    59881 ctatgccgaa catcttaatg caatgattaa agacaggcag gtagtaacca tcatctacag
    59941 tatagggatg ttgtgtatag ttctggcaat aggcatgagt acccgcatag ccttttcttt
    60001 taagtcattg tggtacagga ttttccggat tttttaaaag aattcaacgt ttacttatcg
    60061 gttaatattc ttaaatcggt ttcaggtggc aatgaatcct ctggggaggt atcaagggga
    60121 atattttgtt ttttgtgtac cgttttccca tgagttttgg tatgatttac agaattacct
    60181 gtttgttcaa tatggtgtgt tgccgtggtt tggtggttag agttattgtt acagcttcta
    60241 tatataaata gagtgattgt aagaactatc accaaaatta taagtggtat gagatagtta
    60301 ctggtatatc gttttgcagg tggagagtat ttgcctgaag tatatgatag acggttaggg
    60361 gtactgttat atcgtttcat ataaatatgt tagatagctc attaaacagg attgctgttg
    60421 tatgaaatgg gtactgccat ggttatgctg ttgccttttt tattccatga aactttggca
    60481 aactgtttga ttataaaaat acctctgcca cgaggttgta aatggacagt tgaatgtgaa
    60541 tcaggatatt taaatccctt cccctcgtct gtaatagtaa catataattg attggtatcc
    60601 tttgcaacct caatatgaac gtatttattc tgatcccatt tattgccatg ttccatggca
    60661 ttggttagtg cttcatcaat tataagatac aattcaaggt tatcaagctt cacctgtact
    60721 ttatttttag ccagctcggt tataatatcg tctacaatgg atttgcgtat ttctttaatt
    60781 gtagggtatt tagcttttaa taaagtcatc ttagaatgag ccatcaattc ctcagtatac
    60841 atagtaatgc tttcgggatg gggagatttg aactcccggc ctcttcgtcc cgaacgaagc
    60901 gcgctaaccg gactgcgcta catcccgatg tatatttaca aggctttaat taaaattata
    60961 ccatgaaaat tatactatta aacataaaat ttctttgttc atcctggtta tatattctat
    61021 tataatttac atgatattca ttctggaaat atataatgta aacatgatat atatattgca
    61081 aacatttttt taatcagagc tgacaatttt aggcaaaaac ctataatatt acatttaaaa
    61141 aagaaaatat aatcattatc ctttggattt gtggcatatt cattaatgga tacaataaga
    61201 tgagattcag gtaatttttt aattgttatg gacataacag taaaaatata tatataatta
    61261 tgtatagtgg ttcagtccaa ttatcttaaa aaagtacttg caatgaaaaa cgtattattt
    61321 acaatactca aaagtttatt acatggttaa aaaatgatga aaaagtagag attttcgttt
    61381 gatattgtag ataaactaat agaagattag tattttataa agcatttaaa atgttaaaac
    61441 acaaaaatgg ctatgatcat tcatagcctt cgtttataag gggtgattgt tatgaaaaaa
    61501 aatgatggcg tcataacatc aggtatatat gatggcataa tttatgagcg tctcaatcca
    61561 ttacttgaaa aactggccct ggtacttttt aacaccgttg attttgatga ggaaagttgc
    61621 tcacggatag aagaatgttc cagaaaaggg catgttgtct atgcatcctt ccagagttca
    61681 aacctttcac ttttaatatt atataatctt ctgcgcaggc ataactatcc acttccggta
    61741 tttgcactgg aatacaaccc tttctggttt caggatatag ggtttattac caggcgttta
    61801 tggcaatttt tttcgtctac agtgttggga aagcagtatg aatatgtact tgataccgat
    61861 tatgttgaaa ctttaataaa agataataag agcatccttt tttcattatt atctaaacga
    61921 tacttcctta aacgatatac tgaagttaaa tatgactccc tacggtattt aattgaagtc
    61981 cagaaacgaa tagatgaacc aatctattta ttgccggaaa tgatattctg gaatcgaaac
    62041 cctgaacgct ctggctctcc gttggggcca ttgtattcaa taatcagttc acaggcaaca
    62101 ggtgataaat cattgctgtt tgcatggctg acaattatga aaagtgcaac tgctcctttt
    62161 gtgcgaatat cacagccaat taatttaaaa gatgagatag ccaaagctaa ggttgatgat
    62221 acccggcaat tagctgttat gttgcgcaac aaattgcttg aagattatta tcatgaaaaa
    62281 cgtaccatat tgggcccggt gctgaaatca cagcaggaaa tgatggaacg ggtattatat
    62341 caccccaatg ttgaaaatga gattaaacgt atagctcagg aagatagaat acctgaatca
    62401 aaattgcgta aaaaggcata taaatatttt aaagagatag cagccaattt ttccatattg
    62461 tatatacggg tatttgaaat ggctctggac tggatgttca gaaagatatt tgatggtata
    62521 cggtatgatc ccaaagccat tgaagaaata cgtcaggcgg caactaaggg accgttagta
    62581 ttaactccct gccataagag ccatttagat tatttgttat tatcgtatgt attttataaa
    62641 aacaaaatgg cacctccaca cattgctgcg ggggtaaacc tatcgttttt ccctatgggt
    62701 acaattttca ggcattcggg tgcatttttt atgcgtcgta cgttcagggg attgaaatta
    62761 tatcctacag tatttaagca atatatcaaa acaatagttt ctgaaggata taccattgag
    62821 ttttttattg aaggtggacg ttcaagaaca gggaaggtgg ctttccccaa attaggtttt
    62881 ttaagctata tcactgaagc aattgaagaa gggtataaca ctgatgttgt atttgtgcca
    62941 atttcaataa attatgaccg tattcttgaa gagtcaagtt atgttcagga attgaaaggc
    63001 aaggaaaagg aaaaagaatc tgttgtaagc atgatgcaga gcagcaaatt gcttaaccgt
    63061 aaatatggta tggtatatat caactttaat aaaactttta ctctgaaaga aattaaaact
    63121 acacttaatc ctgtcaatgt tccggaatca gttgctatga atattgtcag gaggatcaat
    63181 gatgttgtaa ctatcacccc attttccctg acaactaccg ctatccttat gtcttcagtt
    63241 aagagctttt cacgggaaat ggtgaagaat aactttgaat cgttatatga tttctgtgaa
    63301 tataaagggt atcagttttc agatactctt aaagatagaa ataattttga tgaaataatt
    63361 gattatgtta ttcagtcgtt cagagaagat aaaattctgg agtcattgaa ggtagtaggc
    63421 gaagggaaaa aactggaaac tatgcccgac atgtatgtcc tgccggaaga aaacaggcca
    63481 aggatgattt tttataaaaa ttctatcatc cattacttcc tgccattaac attttcatcg
    63541 cttgcactta gaataatgaa taaaaaagga gaaacatcat tagattcttt cagaagtgag
    63601 tatgtatata tacgggatct ttttatgaaa gaatttattt atccggagaa tatgctggaa
    63661 gatgtgatgc tttccataaa agacgccatt aagtatatgc agcaaaaagg gtatgctaca
    63721 ataaccaaaa acagtattgc aattaatgat gatagagaaa aggatgccca gttgcgtttt
    63781 tttggaagaa ttttacagga atacctggaa tcatattatg ttgtttgttc aacattgtta
    63841 ccaaagaaaa gagttcagat acagaaaaaa gacctgctca ctgaaatacg caagaatggt
    63901 gtaaaaatgt atcatacagg cgaaataatg ctttcagaat cgttgtctct acccaattat
    63961 actaatgcat taagtttatt gcgctcagaa aaagcattat ctgaaaa
//
LOCUS       DAORYL010000008        53705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_514_length_53705_cov_14.998192, whole genome shotgun sequence.
ACCESSION   DAORYL010000008 DAORYL010000000
VERSION     DAORYL010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 53705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53705
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_514_length_53705_cov_14.998192"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..646)
                     /locus_tag="PLV81_03155"
     CDS             complement(44..646)
                     /locus_tag="PLV81_03155"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOM09131.1"
                     /translation="MLKFSDYNEFLKFCDRIKQVLHQRPPRAIERKSLKDAAVLIPIL
                     LKDNQPHVLVTKRTNNVGTHKGEVSLPGGGIDEDDATPLDTALRETYEEVGIKPDDVT
                     ILGEFDHFISIYGFHVYTFVGIIPYPYDLTINHDEIESYTEVPLSLFIEEKYDDVKYY
                     TYEGTPYAIYYYHFNGYTIWGLTARILTDFSRKVIKNIVV"
     gene            complement(639..1166)
                     /locus_tag="PLV81_03160"
     CDS             complement(639..1166)
                     /locus_tag="PLV81_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019999618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOM09132.1"
                     /translation="MKTIDLHTPIPEHDLQQLKAGDFITISGTIYTARDKAHKKIAEL
                     VAQQKPLPIDFTNAVIYYAGPSPAPPGMPIGAIGPTTSYRMDAYTEAMLSLGVRMFIG
                     KGSRSSEVKKLLVNYNAVYCSSFGGAAAYLNQCVVDAQIVAFEEFGPEAMYKLTVEKF
                     PVIVINDMYGGDLYA"
     gene            complement(1163..1999)
                     /locus_tag="PLV81_03165"
     CDS             complement(1163..1999)
                     /locus_tag="PLV81_03165"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004885.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOM09133.1"
                     /translation="MRTISHEELYTAISNLVVQANFNLPDHVYQALSTMHSSEKKPLA
                     KETLHILLQNATIAATKHIPLCQDCGSVIVFLQIGTGVTIDGNPEEIINTAVADTYRS
                     HYLRPSIVKDPLFDRTNTGTNTPVFIHTDIVEGDSLAITVYIKGGGSENMSSLRMFTP
                     TADIEQIIGYIVATVKQAGPNPCPPLFLGIGIGGTADVAMLNAKKAVLQKEPHPNEEY
                     RRLEEVILQRCNTTGVGPLGFGGNNTVATVRIKEAPTHIATLPVALNLNCHSLRYRST
                     IL"
     gene            complement(2000..2233)
                     /locus_tag="PLV81_03170"
     CDS             complement(2000..2233)
                     /locus_tag="PLV81_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012571241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOM09134.1"
                     /translation="MCLAIPMTVKKIEGTKAIVESMGVEKVVDIALTPDVKINDKVIV
                     HAGFIIEILDPEAAREIEATWDEYTTLLEQEQK"
     gene            complement(2251..3249)
                     /gene="hypE"
                     /locus_tag="PLV81_03175"
     CDS             complement(2251..3249)
                     /gene="hypE"
                     /locus_tag="PLV81_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOM09135.1"
                     /translation="MDIIVPGHGSGGKLMNDLINNLIKSTLGNETIQLDDAAHLNLAT
                     TSIAFTTDSYTITPIEFTGGTIGSLAVNGTVNDLAVMGATPQFISCALILEEGLEIET
                     LKRILVSMKEAALKAGVAIVTGDTKVVPKGKGDRIYINTAGIGVFTNPPQRREIKPHD
                     TIIINGSIGDHGTTIMALRNNLTFSKGLSSDCAALNHLIEDVIRHYPDAIKFMRDATR
                     GGVASVLNEITKNAPFGAKLIEDNLPVKDEVRGVADILGIDPLYIANEGKVVMIADKR
                     CAEGIVEIMKKHPEGRDACIIGHITNEFSGKTYIQTKIGGKRILPVLIEEQLPRIC"
     gene            complement(3249..4310)
                     /gene="hypD"
                     /locus_tag="PLV81_03180"
     CDS             complement(3249..4310)
                     /gene="hypD"
                     /locus_tag="PLV81_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018999256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOM09136.1"
                     /translation="MSDIKKEAALLSQYLLPQPVAIMEVCGTHTTEFFRTGVKDIFPQ
                     SLRLIDGPGCPVCVTANDYLDTAIAIGWQHNCVIATFGDMMKVPSSYTSLAKEKSKGL
                     QVEVVYSPMDAVDMAQKNPDKKFIFLSIGFETTAPAEAVCVLEAKKKNIKNFFLLPGN
                     KLTVPAVKVLLDSGEVNIDGFILPGHVSAIIGKKAWDFIATDYGKPAVVAGFEDHDLI
                     RGTLMLLDLIKNNTPKVLNQYTRVVRDEGNLKALEILYSVFTKGDATWRGIGVIPESG
                     LDLKEEYADFDAYKQFPVDVPPPKEHPGCRCGELLRGVILPTDCPLFGKACSPEHAVG
                     PCMVSSEGPCSAYYKYGRK"
     gene            complement(4303..6561)
                     /gene="hypF"
                     /locus_tag="PLV81_03185"
     CDS             complement(4303..6561)
                     /gene="hypF"
                     /locus_tag="PLV81_03185"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOM09137.1"
                     /translation="MTHHYHIQVKGIVQGVGFRPFVYNVAVSHNLCGSVSNDTEGVTI
                     HLEGPDEAIKQCVDIIRNNPPPLAQIIDIKITKLPPNGFKSFTILQSNTTGTRDVFIP
                     PDVAICHECLEDFFDVHNRRYHYPFTTCTHCGPRFSIIDDIPYDRDNTAMKPFSMCPQ
                     CYEEYTNPHNRRFHTQPNACPQCGPHIVLCDSSGKVIQSQFDAIISQLQTILKHHIVA
                     LKSVGGYHLACDATNDHCVNLLRMRKGRPFKPFAIMVSSLQFLETFCLVTQKEKELLL
                     SKERPIVLLKLQKDIVSPYVAPGLTYIGVMLPYTPFQHMLFSSNDESIYVMTSANISD
                     EPIIYNDDDAFERLGTIADYFVTYNRQILSHTDDSVVYVVDEKPYFVRRSRGYVPAPI
                     FIKETPLHILATGGDLKNSFALARNNVAIVSQFLGDLATPWGNDLYRKTIAHFCKIFD
                     VKPKLIAHDMHPNYFTTMFAQEQTTLQTVAVQHHHAHIASVCQEHSIEEPVIGIAYDG
                     TGYGTDHTLWGSELLIADTKEFIRAAHFDYFGLPGGEHAIKDPWKIGLSLLMTSSVDD
                     STFFTQKQEKEFVKEIVYKTINCPATCSIGRLFDGVAAILGVAHHISTEAEAAMLLEE
                     KALEAIKDTTVFEVPIKQEQTLVIDTAHIIQEIVNMIELHVPVAAIAMRFHRAIAEVT
                     ITTACKLREHYSINKVALSGGVFHNRILLHLITTGLAKKSFDVYTPHKLPCNDGGIAL
                     GQIAIARENIHV"
     gene            7037..7441
                     /locus_tag="PLV81_03190"
     CDS             7037..7441
                     /locus_tag="PLV81_03190"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOM09138.1"
                     /translation="MSKIRLFVVCLGILVIAVIIGCSEGTTNINPSPTDVVTVTDINK
                     FITEKDKLAGIAATMNAMDPVAMPHKKHEDAGIKCVVCHHKAGNDDRIKQCAECHKGA
                     KGEDTIHGLCINCHAQSKKGPVLCNECHIAKE"
     gene            7448..7930
                     /locus_tag="PLV81_03195"
     CDS             7448..7930
                     /locus_tag="PLV81_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HOM09139.1"
                     /translation="MADSAMKRGKKILILGIGNLLQKDDGIGVHVVQYMQKNNVSLPA
                     NVELLDGGTAGFDLIPYMIDFDKLIIVDALKVQDRPGSIYRFDGKHLKAQTPNVSLHE
                     MGIAEVLKVLKVQGYSPEVEVIGIVPENINTLDITPSPSVAESIPKVVDLILDSVTAY
                     "
     gene            7955..8959
                     /locus_tag="PLV81_03200"
     CDS             7955..8959
                     /locus_tag="PLV81_03200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HOM09140.1"
                     /translation="MSLNRREFLKVMGATTAAVAFPSVLIQGCKRALQKASERTNVIW
                     IQAQSCSGCSVSLLNKLEPDIATVITEYISLNYHQTLCGGTGHAAIQVLENAVKTNRK
                     DFILIVEGSIPTKSDEYCTIGEMDGRIIGAREWIEKLGKNAIALVAVGSCATFGGIPG
                     AKIRATGDNPTGAVSLQKLFPDKKVINIPGCPPHPDWMVGTLLHVLLKGMPELDEYNR
                     PLMYFGTTVHEKCERLPDYKRGRFAKHWGEEGCLYLLGCLGMDTGCDIPTRKWVGGMN
                     TCTGCGSGCIGCTEPPFPDYGNRGIYKHLVASNDELQTLHPEIASTVVKLKNGGVING
                     "
     gene            8952..10517
                     /locus_tag="PLV81_03205"
     CDS             8952..10517
                     /locus_tag="PLV81_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOM09141.1"
                     /translation="MAKVLLDPITRIEGHLSIEIDVQNGRVVDAKSKGDMFRGFEMIL
                     KGRNPVDANQITQRICGVCPVSHGIASSKCLDAAFGIVPNKNGRLLRNLVFGANYLQS
                     HILHFYHLAALDYVDITAILKYNGSDKKLLALRDWAKNELEVKKGRQDAITAVAPFLP
                     RYEGDFYIKDLDLNIAAIAHYVQALDMRMKAHQMVALFGGRVPHLIGLVPGGVTQVPT
                     RSAIRQYRKLLKQVEEFVNEVYINDVIAVAKAYSDYFKIGRYTNFLSYGVFEENPEQT
                     EFFFKRGVFDGKKLQEFDSSKIREQVRFARYNSGSNLHPYQGQTNPDPHKAGAYTWLK
                     APRYDGMPMEVGPLARVVVNYLGGNADVKKEVDALLKVFNADIDAVFSTLGRHAARAI
                     ECKLLCQRMYEWLDDLEVGEMPRSTYEIPDKGQGEGLTEAPRGALGHWIVVEDKKIAN
                     YQCVVPTTWFCGPRGDDGKLGPVEQALLGTPITDPRNPIEAARVVRSFDPCIACAVHV
                     VEGDKEIGTFKIC"
     gene            10669..11877
                     /locus_tag="PLV81_03210"
     CDS             10669..11877
                     /locus_tag="PLV81_03210"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM09142.1"
                     /translation="MSIIRDVTLEALNYIHNDDILIFIGARQTGKTTILRQIQKSLEK
                     ESTAVYFINLEDPDYLSLLNENPKNLLKLFSFDLNKKSYVFIDEIQYLTNPSNFLKYF
                     YDEYKGKIKLCVSGSSAFYIDKKFNDSLVGRKKLFYVRTLSFKEFLRFKNENKLIDND
                     FKNLSITEKEKISFYYHEYMLYGGYPKVVLSDFDQKIDLLMDIAYSYVKKDIYEAHIK
                     SNEVFYKLCKILASQIGGLVNTSELANTLGISKTAIDNYIYILQKSFHISLVRPFYMN
                     VRKELTKMPKCYFMDTGLRNFFVSSFESFKTRKDKGQLLENACFRQLVDSNNEDNIKF
                     WRTIQGNEVDFIVNEKIAYEVKVDENQFRKSKYSVFMQMYKSIPIAIVTLDKKVEMVE
                     GCQVFDVWEI"
     gene            11984..12652
                     /gene="hypB"
                     /locus_tag="PLV81_03215"
     CDS             11984..12652
                     /gene="hypB"
                     /locus_tag="PLV81_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOM09143.1"
                     /translation="MEIQVLQNIYESNIQKAEEIKKLLSQKKVYMFDIMSSPGAGKTT
                     ITDRIINDLIKDYNIALIEGDIETTNDAQRLAKYNIPIVQIETKLFGGACHLESSWIL
                     GCLEKFDLNAVDVVIIENVGNLVCPAEFELGDDERVVVLSVTEGEDKPVKYPLMFNSS
                     HTLLINKIDLLPYLDFNMDELLANVKRTNPKMEVHKVSAKTGEGIEGFLKALRSRIKN
                     KVGR"
     gene            12757..13671
                     /locus_tag="PLV81_03220"
     CDS             12757..13671
                     /locus_tag="PLV81_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOM09144.1"
                     /translation="MTKTLLWVGFNVFVLLMLLLDLGVFNRKAHEIKVKEAVLWTIFW
                     IVLSLMFNLGIYFYAGYHKALLFFTAYLVEKSLSMDNIFVFLMLFTYFKVHPMYQHKV
                     LFWGVLGALIMRAIFIFAGIALIEKFDWILYIFGAFLIYTGYKMMKEKDKEIHPEKNP
                     VLKLARAMLPVTSDYRNGYFFVKENGKRFVTPLFIVLLVIESTDVLFAVDSIPAVLAI
                     SHDPFIVYTSNVMAILGLRALYFALAAVMRLFRFLHYGLSVILIYVGCKMIAAQVGYH
                     IPTEISLGIIAFVLTASIVTSIYIPEHK"
     gene            13739..18040
                     /locus_tag="PLV81_03225"
     CDS             13739..18040
                     /locus_tag="PLV81_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOM09145.1"
                     /translation="MITVGINIGSTSVKVVVCNSGVVETVNIMPHEGDVPGTLTTILS
                     QLNIPKDARAIVTGNEGRFLLHLPRVIESVCIEEALKHSDYRPDAIVSMGGEDLVVYT
                     VDEHKKIKNNFSGNKCAAGTGEFFKQQLARMNMKLADIHSIPHESKVLRLSTRCSVFM
                     KSDCTHRLNKGEATKGDIILSLSNVMAVKVVDFLKKARIHNGTVIITGGITRNPYIVQ
                     FIKEKLPDIDFIIPDTAAYFEAYGAALLAQQYGEPIGDTLFEKHTISFPRFNSLKTAL
                     NNVTYIPSKRGVAKPGGEYILGVDGGSTTTKACLVDIHTHEIVAAHYGRTHGDPVKAL
                     KQCIEEIQKQLKDQIGDNPITISLAATTGSSREILGVFLETSAIYNEIIAHSVGTTYF
                     KPDIDTIFEIGGQDAKYVYLKNGVPIDYAMNEACSAGTGSFLEESAQGDLNIAHAWEI
                     GDIALQAEQPLKFGEHCSAFINSDIRKAIQQGASREDITAGLITSIVANYLNRVVGNR
                     TIGNSIVLQGGVAKNKAVPLAFAMLLGKHITVPPDPELMGCFGVAIVALNKLHDGLLQ
                     KKFYSLDAILNTDIVYEREFTCKACDNYCPIRVLNVNGNRYMFGGRCNKYANMRKKRV
                     IHEEEVVDFIEKRNELLFHECAPQEMPHNASITVGVPLCFSAYTLWPLYSWFFKELGV
                     AIVTSDAISEEGKAKVESAYCFPAEIAHGAVKNIFDKHVDYIFLPHSKEMKSYQNDAR
                     ATFCPITQGLPYYIEKAFDEIPKEKILSPVISFKLSDTITEDSFVNVAVKLGFSSEEG
                     KRAFRLALQKQHEYFVKARKLGEEALEFARKLNKPAIALLGRPYNAFTKDANMGIPKK
                     YTSIGYTVIPFDILPFEDEEIFPNMYWFYGQLDMKASVMLAKENNIYVTFISNFSCAP
                     DSFMLHYLKWIMGSKPFLILELDSHTADAGIDTRIEAFLDIIEGYRQKCMGEETQRYD
                     NGLRFVIEDNELYVRNIVTNSKIPVRNNKKVTLLLASMGRLGAELLAASLRSAGINAE
                     AMPVPDAKTLQLARQFVSGKECLPSHLVLGSALQYFASPRYKKDEIYLLFVPTTQGPC
                     RTGQYFVFYENLFKDMRFDNVCVVTLDSSHSYTELGPHFSKHAWWGVVISDYMKDIET
                     SLRACAKDPVSAMALYDQLWQELISVAEKDVTRVIPVLKKIAQTISNIPLQRTIAQCP
                     KVLIVGEIYVRRDDFAVDELVKHFSKKGIIAKVSGVSEWIYYCDFIREYEYKKAIKLA
                     PWYKKLFIPEVWSLLRFFIEQWYKHRVEKKVKQALQRTQLIPDTPHDMHHIMDMSQKH
                     FVDLELQSEITVSSGVAATAMQDGYSGIVNISPFACLIGRVIEGILTPWARIQGYPVM
                     SVEIDGNALPPTTLNKLEIFMLNVMRFKEKPEMKEVVEHPAQTAVAIDRKIIKDL"
     gene            complement(18045..18419)
                     /locus_tag="PLV81_03230"
     CDS             complement(18045..18419)
                     /locus_tag="PLV81_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09146.1"
                     /translation="MSTLNDLFSSEFLKDNAILIGKIKKPAITKEEANQILEFVKQDI
                     QNHPDHQHFILDVLSVQEVSNPIIGVLMKALQFWKKTRGYALLVMTDDLLQKIMIEQP
                     AMFDFFAVFHTVDEAIAFTKKA"
     gene            18553..21276
                     /locus_tag="PLV81_03235"
     CDS             18553..21276
                     /locus_tag="PLV81_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09147.1"
                     /translation="MKTFIHYCIKILSYSVIALCSLVVFVMLILFIANLVLTTDRVSK
                     IITDEVESNLNAKVSCHVTHFSLFNGFEIKNFILTGSDSSKPVVTFDTFRLRYSFFPL
                     LIGKIKIYEVGLYNPSVYLEEQKGIWNIQKLSKPSEKKEKEDKEEAKEEKEKKDEITL
                     PIPVEVLFGLVLENLSVTIDSSTYTAALKNFSGSIQLHIPPARRIPLNILALALFDKL
                     KITVNPEETLSLSFVSRDITAGPPLLFTFNLVYHPEGNQLFSSFKCGTYNTPVRFARK
                     HVAPLNAKIEYDIAYNSSDDAVNCKILRIVFGKSTWITFAGTIEHVNSKPEIQFAMLD
                     SNIRLNEIYEYTNVLLGPKPYFNGSISIFPLTIQGNIHALTVKGAIRGQKVAFATDGF
                     AVTIPSVEIPYTVVLQNSVIDASINIGLPHFIYTLDENRSRDNGISLQCNVVYNLNTS
                     MLDLQRIVLIHRAPEIAAETMRVEVSGSVILGDIVAARINMSTVRFDLPSMAYTMPAP
                     LKQSLTSSPITKPVTVRLKADVSTSAQTNSINLRALGVIPDYSIDDLTISASVTQKPQ
                     KGKIDIAYCDIASTKHQASIYMKGSVITKEDVYLDVDAGVHVAPRAPISFGNYTVSGV
                     MKIVLSLQGTMQSLVAKGSLQSQQLILSNGAGKLFVGPVDMDIPIAYTMGQSFKMPFD
                     SADVMNIQLFKQKANFSIGKIMAPHPSRNIAFEYVKNMHGHIGFKRNIMEIKNLQAEV
                     LRGTVTLKELNFNLADLNPQHMEFRVALNANDIDIGKLDKPESVRINKDALLSLNAQV
                     VGKNLNIAQDFDISGSINIYKVGEDFANRLFKGINEERGKSKLGMGQFAVDNSLIITG
                     FDFYVDKGLVYPTVFFKKKMLGIFVTVNNEQVRYERIPVVEFFNRVREETL"
     gene            21273..21869
                     /locus_tag="PLV81_03240"
     CDS             21273..21869
                     /locus_tag="PLV81_03240"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HOM09148.1"
                     /translation="MKHIVAIALAIVAGIVYSQARCSSSSCLKGVPSCIEIQPPAIHI
                     TGEKTALERQLIGEYREIEKDAWVTSTVMVPSKGERRAMVAVDETMLQAIKLRELNED
                     KIRKYKDMGIIGENNNGFVGIVDEKKLQSMKESTVIRTLVNDENNARQIIFIKSSDSK
                     DARDPKAIQFGYDFAKEQHAKAKKSDWIQKEDGRWVKK"
     gene            21866..29242
                     /locus_tag="PLV81_03245"
     CDS             21866..29242
                     /locus_tag="PLV81_03245"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM09149.1"
                     /translation="MKRIACALFGLLITGCASTAYRDIPFDYSQVLTKEFRFGTGRSI
                     PLTFERSVNVSGSITSDGKYFIYASNKERNNFDIYLRALNDIKTVRLTIHPARDFAPD
                     VSPDGKWCVFVSTRDDPEGDIFIMKIDPEKIVTQGDQEVPSNNITAIRDPLSGAITPV
                     RDASPCFSPDGKLIAFSSTRDGEESIYIMERDGTAIRKITEGMQPRFSSDGKELIFVK
                     KTNTGSVVFTINIDTGTVTQITSGKYLDLSPCFGKTSNDIYFMRIQYDTNGDSILDSN
                     DKGMLFAYDVQNKEEYPLTLIEEPSATPRWVPYADGIIVYSIVEGQYINIAMIPHTGI
                     IPKQKNALRQYELALKYKEDFDDIDRYQRCLFATYYFFNESSDVDSQIIIARALYELS
                     HVQQYSFVITLLSDLGKRSKPAQIYMHYRTVKDREKLPYLEQVVKELSTQNEEKIAPF
                     FMEELADQYAGLQMQAKAKVLYNEIIARYQNYTRLMYVRYKAGEQLYKEFSIPQEWIT
                     VLQSSYTYLKNAITLSIIEQTKKIPSKVRYEHALKSAETYNVPVIKAIMNYVAATALM
                     ELSQNDDAIALLKQGLEYTKKLDVAYFLINVTLGKLYYEKQSDEWVVYYEAAVNNYQA
                     QWKQDIRPIIEQMAEYFESTGQRLSAEGNFKEAAAVYKRSITMNTLLHLKRRYKDIYS
                     ANAPGAHTGYINAMLAMSQATAEHSIEKEYLERLPIARMDFDKAHIYGLGYIYVKQAK
                     ALAPSPDAMDGRSSQEFETILQLFTNALRHIDWSLFIDDTFVDAYLLKGWIYQYVDEL
                     RVRFPSKKRLIDSYFPEYLWEKSIPLYEKALAANSENLFPQKEADIHCNIGNIYFLLK
                     NFNLASRHYAEAIKYTKTFSTLKEEALFYYHYGYCLWQQGNYSGAYVSMQKAFTIYTA
                     IAQQNKSAIADKLLVLYKYFALFERMQGNYEKAIEWYKKTLEHCAQFQCGDSSSRLYI
                     DIAECYRRLEQDSEALVYLELAERSLPQEKSVQYKIGWKFAGFGPVYFYDLGQDTVVI
                     GEGKIVSHLSGFEMEILIQNMRADIYAKSGRFGDAATKYLDLVAITKKQKTTLIADAH
                     ITALYNAAYCYTRMGDVQKALELYGTALSIAQDSNIPIDKIYQTMVQWAYCYTASHEQ
                     KAKQVESLDKAIRTTEQYKKNMYDKVYNSLLDDYTKKMKAQKKKPVDEDIAALKQQAA
                     KQVEAYSIKFSALTALLYMHKASLLEQDITSDKSTDYYVKANEIIGYYSDAERLFSKA
                     INYFGNDASHQEFVIKLLINRGICRIHSGQFRDGYDDIISARFKANKIQSKELVWIAE
                     ATLWDLANTYSEVKQLIDNPDRLIDGVLSRVELLPPLYTMKKEFIQKLYDTYADELIQ
                     KKKYKEAYAIQQRRQIITLAFSGLSIPKFYKKSDNTIYSKFLRTCFTIQDLEQKLTSA
                     IVNEEEPDVIEKLTSTISYHYKNLTGYRSAASRFLLPYLGVYTPKASEYTIIHFYTFD
                     NKLYGWIVNKNTIDWLLISDSLKPERLTEDLNTFIQTLHGKVYVVLNNTFIALHKYIN
                     NTVLYTMGCVPFASRVDGIERLVWLPADVVTTDTKLSSNIHTTANINNSEYIVDTDNP
                     DITITPPYCFANKLECSAVFKSINNMKTDELMLLADASFYSHIASVILYSGDNVETLL
                     TAVINNDIKLINSLKTKTVVLGKIPDTIKAREQNAQIIVQEKHTEYAKALERGDYDSA
                     LSALERWKRAGLQQQEYYDKKILVLVYHNNIDEALKVMQSHSSAFTSSFSVYTYLLKG
                     DVTSAEKQLNNPHLAGTFDYTVYESIIKGYKHKEKLTVINNVSKSTASILPANQLLLL
                     YAEEAFLLGEKEKAKHIMELYQADYIPSQRELLVAYNLGYSIASHSNTVAYRMLKAIT
                     GNSNSSNFINTINEYNNLLLFGILEIVKNRPLMPDEEWQRIMLIAEKGKHMGIDVQFT
                     TAKIAETLQGIIPARVIQYIKTVAVDKGKIVSPIVNLYAINAYQKTDNFNDAYTACKT
                     LKQLPYTLEQLWYTYYIYSAIMLGKTDDAQELLNNSLIATWDLVMYNVFNVCIKLQKV
                     VALKTTDSNMLQTIAQNIDVLITSLHDESIRWYYSEYKYLLDKLINYAISYAMSRGNQ
                     RDALKYAELHKMIEGIALFDENSSYKSMYNDLIGNKFPIMEIQKSIPRKTAVLYTTKN
                     ENDIFAWVITRTYIQPVRLQNSFKQLYDIIHDYYNDVGLLQQAFDKEAAINTIMQPVI
                     KELSGYTTIIIIPDAYTESIPYENIQYNKGDTAIVFMPSLMAAMDKTSSIPPIAYINV
                     NNTKKDLIVSALKQSDFAYTSDATVKSIIITDRQISYNNVKKGLMINGDVIQSGNVFI
                     NLANTSQVSYYYCLFMIQKEMGISVLVNSAVSDVNSIIFIREFCNTIKTDSALNAYIS
                     AYQYLKKDRRFKNPAYWLGIRLYSRTIKGIYDGVNK"
     gene            29239..30444
                     /locus_tag="PLV81_03250"
     CDS             29239..30444
                     /locus_tag="PLV81_03250"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HOM09150.1"
                     /translation="MKGQDMPTAVIIDDDEMVNEVLKFILENDYNVYNYYTAEDALKS
                     DVIASADIIITDVNLPGINGLEFIHKVHEIDENVPIIVITAYNDIEVAISALKAGAFD
                     FILKPFKNDQITLAVQRAYERHKLVKENIKLVEELKLKNQELEKLYAQLQARSIQIEN
                     ELDIASNLQQCLFPVVFPEIKGFSFYLKFKPVEKISGDFFDFIIHDEKHFSFIFADVS
                     GHGVPAALYSAIVKTAITTFVDISKSPSDILKEMNQFLIQTQKMMSYNYATIFFAHFD
                     LAKSCIEYVNAGLPSPIVMHPDGHIIRMETTGPFVGIFDTSLYTTKKIELLPGDKLLF
                     FSDGAFECPDRVDRILGEKRFQELIDEHRDLEIPELIEFLFDKIQDFCGVDRFVDDVT
                     MLGMKFSTH"
     gene            30552..31580
                     /locus_tag="PLV81_03255"
     CDS             30552..31580
                     /locus_tag="PLV81_03255"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOM09151.1"
                     /translation="MRKFMFIYWVLIVTVLLFAPYVYALEISFVVGDVIVERDGKENL
                     AVVGMKLSQGDVVVTKNASIAELLYSDKSTLRIFENTKIKVAAIQDNQENVSVINGVI
                     KAKISKLQKGESKKVYTPTTVCAIRGTEFFVAVSDSSNSRVNMEEGSLALANPYGEIS
                     VNNDEQATVEVAKKPLLDDNPKNPELWKNTADKDFVLEPQKYAESYNEYLSTLEQRSS
                     MQSAEIEKYKQSSAKKKDSKKIEKDMDELENKVAVIADDFFLGDCANAAVDNVLQMYK
                     EKKQEIFDSFNKVKEQADRVVQQQKANYEAIMAIKEAYRKNYEEIKGKHKESIEKIKE
                     EINKVHDQ"
     gene            complement(31588..32499)
                     /locus_tag="PLV81_03260"
     CDS             complement(31588..32499)
                     /locus_tag="PLV81_03260"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HOM09152.1"
                     /translation="MLIVSPIIRYIKKQSQKKSFPKKKVLILEGGGMRGVFLTGVLQA
                     FTDRDYFPFTLIIGSSAGALTGVAYSAHQIYLARDAFFGVLLSESFIKISNILRPTKH
                     ILNLDWMVSTVIEGKETLNIQYLKHSIPVLITATEYSDNNPPSTVYLHSRKDDIITSL
                     KASAAIPVLYRGFVEYNNRKLCDGGLLDPIPYKKALNLGYKEEDILVVVTRKKGYRKK
                     EESFWIRYLIENYFKDSKYRYLIQALENRYLMYNSILDELEHKHKNIDIIYPPDDFTV
                     DRLTRDPKKILEGFEQGVQSGKNFLLS"
     gene            32828..33313
                     /locus_tag="PLV81_03265"
     CDS             32828..33313
                     /locus_tag="PLV81_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOM09153.1"
                     /translation="MEAIVVQHGEAVDASVNPERPLSKKGQEQFVRLAQFLKIINWKP
                     EIIIHSGILRAFESAAIVAETFNCEVAVNKSINPKDNPDVIIQYLLLEKRNIIVVTHK
                     PFIDILVAKVLSTNQTGLITVTNASPIFLSSDGDMLYCKAYIPEMMIDAIIRSFSNRQ
                     S"
     gene            complement(33282..34262)
                     /locus_tag="PLV81_03270"
     CDS             complement(33282..34262)
                     /locus_tag="PLV81_03270"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOM09154.1"
                     /translation="MKKARYIFLFIGILILVFLFKTFGLQKTIAQILSVGWGFWIIVS
                     IFIFSHIFLTLGWKVLINHPVTLKTFILLILARIGGDSTSSINAFGSIAGEPLKAMYM
                     KDHIPLQIGLASVVLDRTAHSLANALMFLTGITLSLFILNLPVYITVIAVGFFLLTLV
                     IIALIIQKQREGLLDFIISMIPSRLSSKIITDGRRKKIEELDEEIKYIFSSKSNIHKF
                     IISILLHYFAVTISCTLEIYLIVYFLNITFNIYHALFLYVFGLFLTSVMFFMPANLGT
                     SEGSYSLALKLLGYDPSLGLTIGIVRRLRTFVWSIIGIIILMIAGLKKNE"
     gene            complement(34279..36027)
                     /locus_tag="PLV81_03275"
     CDS             complement(34279..36027)
                     /locus_tag="PLV81_03275"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOM09155.1"
                     /translation="MLQYDIEKFSAINKRINSNLKLSDLLMVILDITKDMLQTEGCSI
                     LLTDPVTEDLYFYIVRGGSDSLIKGEIVPKGKGIAGHVALTNTPVIVNDPQNDERFYK
                     EIDNKAHFHTRNIICAPMVLVDRLVGVIEAVNTIGRDSFTQDDLKVLMYIADSAAIAI
                     THRQLYQDLTTRLKELEIMYEISQAIAHSAPSENIFSKIIQPLASSVGVKRASILLYN
                     TSTDKFSIASSYNLPESINSPLSKNQAPIAYHVFTSGDPLLVNNIIADPSLASFAKIH
                     EYITPSFISIPLRYKNTVIGVLNLADKLDKTSFDAYDLRVATTIANQIVEAYQNSLYL
                     QQTEEQRRLAQEIDIAAEIQKRILPKIPQSINNHHLAAFNKSAKEVGGDFYDLFQFDN
                     EKFGLLIADISGKGIPAALFMGTARNVLRAESRINNQPGQLLTHANKYIYEDSEYGMF
                     VTVFYMLIDSHNKLLTYGSAGHNNQLLIRSKTLETIRLNAKGVALGITNNAKYEEKAL
                     FFEDGDLVVLFTDGVLDLLGIGNMDAGEKKLIEIALQSIKKGPDFLIQRLQDNLVTQD
                     VDKEFVDDFTVLAIQF"
     gene            36284..37843
                     /locus_tag="PLV81_03280"
     CDS             36284..37843
                     /locus_tag="PLV81_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09156.1"
                     /translation="MKVIVELSLTPDFKEEELSKELSLLCGCTITSYSILRKSLDARK
                     TPVYRYRCCVDIPHHVATTLISIGKATAYKEEEPVIIPKVKTNKRVPIVGCGPAGLFC
                     ALTLIEAGVAVDIYERGKTLAQRHRDVAMLREKGILDANSNVVFGEGGAGTYSDGKLT
                     TRIDKKEIKLFYDFLVNFGAPSSIVYEAKPHIGTDRLIGIIENIRKYLIQKDCTIQFG
                     KKLTDLRIHNDTLQGCYFNNTEEIVTEILVLATGHSARDVYQVLHARGVALQKKGFAV
                     GVRVEHPRELIDEIQYGKGKDGYGLHGAEYQLTFKDVTGRGVYSFCMCPGGFVINSSS
                     QEERLCVNGMSYSKRDGTSSNAALVVTVSQDDFKDIFDGINFQILLEEKAYQAGGGGF
                     GAPAQRITSFLQNSIDSSLPVCTYKPKIAASNFNTILPDFIRDPLHRAIVHFGSKMKG
                     FITNEGILVGVETRTSSPIRIVRDENFQSINCKGLFPVGEGAGYAGGIVSSAVDGIRA
                     AQKIVTLLERV"
     gene            37848..39725
                     /locus_tag="PLV81_03285"
     CDS             37848..39725
                     /locus_tag="PLV81_03285"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOM09157.1"
                     /translation="MIPVLLRLKNPINIDDRHHFPRPDLLRLPTYNLNGQWVIVPDRK
                     NRGTMLQWYTKTNVQKILHQWYNIDEFESYPVIVPFPLEADKNNEFHAQSGITPEKVI
                     SHKRYWYFCSFSIEDITTGILHFGACDYKATVWLNGKLLGTHEGGYTPFSFFTHFEDT
                     NYLAVLVEDSLSPSQVRGKQTFLNKPFMVWYTNITGIWQDVWVEKVGSSYIRSVTCQR
                     TGSSVRFIIDVVTSENIANDFRVELFIYASQVHGKKSALKTPVKTYRDIVMFDVFKKG
                     QVIFEVPESLFATWSPQYPALHPLQIVLKKGTKEADCVYSYFGRRDIEIKNGSILLNK
                     KNLYQRLLLNQGYYPEGLYRPGDSSMYRTDIELMKSMGFNGCRIHQKIEDPKFLYWAD
                     LLGFLVWEEMPSYYLPLPKNMKKLYTQLQEVMKRDCMHPSIITLVLYNESWGLYSMFF
                     SKRVRDSLMALYTRVKSDFPGYMVIDNSGFHHVKSDIADIHHYLKRFEDIEEFYRRLV
                     AGVREAPLWANFISMILGKENIQTPYLKGYADSQAPLLISEMGGFGFGMYQSEAKTLE
                     ESFQKHCAILAKFPHIQGFCYTQFTDTFQEKNGVCTFDRTMKAKLLPIMQKALSKHIF
                     W"
     gene            39792..40703
                     /locus_tag="PLV81_03290"
     CDS             39792..40703
                     /locus_tag="PLV81_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09158.1"
                     /translation="MKKFALIVVFILLASAIYPEGGDYLRVTLVSPKLSYDENDDVIV
                     YCIVHNTAGVPVEFTVYDAPYTTFQPVVYALDGKEASLKVDYRLKNIPTAQLLANEQG
                     RKIILQPDEKFVYTAYCNTFYALTKNEKYRVKAYFYPDAKDDYVIKSANEVTITIIPV
                     PQFAMRTGIIKHERKVTPSEVVMLALMAEKNKQWENVVKYINIEKFIYAYSEFARRYS
                     TTNDYERLIVQQDFINFITKERPDYLIDFSILGEQIIPQTNVAYVDVKVKRWGPRFPA
                     IMKYRYTLEPYKDFWVIVNLDASIVKE"
     gene            40707..41729
                     /gene="fliM"
                     /locus_tag="PLV81_03295"
     CDS             40707..41729
                     /gene="fliM"
                     /locus_tag="PLV81_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HOM09159.1"
                     /translation="MTEILSQDEIDALLTAISTGEADTTDYTAVKEQRKVKIYDFRRP
                     DKFSKDQIRTLQMMHETFARLTTTALSAQLRALVSVHVASVDQLTYEEFIRSIPNPTT
                     LAVINMDPLKGSAVLEIDPSITFTIIDKLFGGTGESTKISRELTDIELSVMEGIIVRI
                     LGNLREAWSNVIDLRPRLGNIETNPQFAQIVPPNDMVVLVTLETKVGEVEGMMNLCIP
                     YITIEPVISKLSAQYWYSSIRKGATDENAAIIQSRLEAVELPIVVEVGEVEITMEELL
                     AIQAGDVIKLPNTKINSDMILKVGGRKKYKCRPGVIGNRVAVQIGEKIEEIPDELLVS
                     KRSEEE"
     gene            complement(41748..42758)
                     /locus_tag="PLV81_03300"
     CDS             complement(41748..42758)
                     /locus_tag="PLV81_03300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOM09160.1"
                     /translation="MGLNPFKESNISDLQKQLQDLISERWGHIRNKLLTQWEYIKKRG
                     NERLTIMVIPHSEKKIVNLHVTLFALTGIIVSLILILTLTSIFIINHTSTIKEVSRLK
                     MYGSNSKIQIAKYKEEINKLYDIFQRLKPELSYLYSLTPGNRADTLWAKGGVSENIDV
                     EQEQPSPPIEILNIEEMVQDLETAKDVLKDIKKFMRERRKVIENTPSMWPVDGFVIAR
                     YGIRTSPYTFQKEFHEGVDIASFPGAPIRVTAPGVVESVRWDSQLGLSVSIKHKYGFV
                     TVYSHCQRVTVEEGQKVTKGEVIGYVGKTGNATRHICFYQIKIGTEFVDPLPYLNKVV
                     NQ"
     gene            43439..44290
                     /locus_tag="PLV81_03305"
     CDS             43439..44290
                     /locus_tag="PLV81_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002764028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOM09161.1"
                     /translation="MIINHNLTAINSNKVLKFKHWDVDKSMEKLSSGMRINRAGDDAS
                     GLAVSEKMRTQIQGLRQAERNTEDGMSFIQTAEGYLNQTASILQRIRVLAVQSSNGIY
                     TLEDRQLIQVEVSALVDEIDRIASQAEFNKFKLLLGDFSRTNPKASMWFHMGPNMHQR
                     ERVYIGTMTSLGLNLREKTGKFLANLSTAEGANRTIGIVDEALQKISKQRADLGAYYN
                     RLEYAAKGLMNAYENIQASESRIRDADMAETMVDLTKDQILVQSGTAMLAQANLRPRT
                     ILQLLGT"
     gene            complement(44361..45989)
                     /locus_tag="PLV81_03310"
     CDS             complement(44361..45989)
                     /locus_tag="PLV81_03310"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF011443.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HOM09162.1"
                     /translation="MEEIIVIGHKNPDTDSICAAYCYAHLKNIIDTHNHYTAYRCGNV
                     NRQTRFVFEEVNLQPPEMLKDVYPKVSDIMTRNVITAHPDDPLLSIVKHIEKDRVRII
                     PVVSGDSYYKGVVSFAELAEFYFSATSIAQPLYYFKPANFTTTIPGRCIKKGQNEEFE
                     GHIVIGAMESAQSLNVLKNVKPEHTVLIVGNRKSIIEFAIHNNFAAIIVTGTQDFNYD
                     TATYSGWVYLSQLDTFNTLRLLTLSIPCHYIMKQSDTILPQSTVMQAREKIVNSEYRG
                     LPVCDNGKLTGIITRSDLIKHYAKKMILVDHNELSQAVEGAETAHIIEIIDHHRLGAI
                     KTTTPIFVYSKPVGSTCTLVYELYKYNAIQPEPHIAMLLCSGILSDTAMLLSPTSTDI
                     DKKALEELSAIAGIHYEEYGKKLLSSTESLKTIDPMYAVSNDCKIYQEYGISVAISQV
                     EVITLDDIESVKESLLSALSQFAQSRNVHWALLLVTDITTQNSVLFSTNFTGEQKLSF
                     SPLGPHMYNLPGILSRKKQLLPEILRILEELSHA"
     gene            complement(45997..46887)
                     /locus_tag="PLV81_03315"
     CDS             complement(45997..46887)
                     /locus_tag="PLV81_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09163.1"
                     /translation="MKSYPFYFYTGILLILFMQICLLLDIRMVTIWATPTLWTGLILL
                     TDGILAKTNRSLIQNRALGTLAVISVLSWWMFEWFNIYFSNWHYQGLLQTLHIRYLGY
                     IWAFATIFPGVLLVYGIILTIIPKILYKQFHLTSSFLIASFIIGLFFLFIPIIPFSMY
                     YVNRAADPDLFIWLSWSANTFYSEFTAGFIWLGFIFILEPLNYLLNRPSLYAELVLGK
                     YTKLIALMLAGLLCGYLWEFWNWWAATKWIYTVPILPHIKLFEMPILGYIGFATFAWE
                     IYAITALVYPKAITILEREH"
     gene            complement(46918..47781)
                     /gene="metF"
                     /locus_tag="PLV81_03320"
     CDS             complement(46918..47781)
                     /gene="metF"
                     /locus_tag="PLV81_03320"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOM09164.1"
                     /translation="MNIITRLQQKKPLLSFEVFPPKTPIGEENLFHELHVMVSFKPDF
                     ISVTYGAGGSTREKTLEIATRIKSTFSIEPLIHFTCVGQSKDDIASYLNHVQSLGFNN
                     ILALRGDPPAGQHKFIPHPEGFKYASELVEFIRTMDHFTIGVAGYPEGHIEAPDLDTD
                     IQNLKKKVDAGADFIITQLFFNNDRFYTFMDKTAKAGISIPIIPGIMPLTNMAQLQKV
                     TTMCNPDMPVALLQELEKCTLPDDMCKVGIEFTINQCKELQSYGVPGFHFYPLNKSAA
                     VKSILDEFWHG"
     gene            48206..48937
                     /locus_tag="PLV81_03325"
     CDS             48206..48937
                     /locus_tag="PLV81_03325"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HOM09165.1"
                     /translation="MRREVKAFILSLAIGSLVLIVGGGNIMVQAQEAQQPKKQQTGLV
                     EDWMDDFEACEDWRAASTCPIGETKIRKIPGKPRPIDDNGNEINYPDTITDDNGITHE
                     NKFVLGVKTYYLDRGYDRVEVFPPNEYIIRGKAKEVKVWALGRKFRHTLFIKLRDYKG
                     TIHKIKIGRLDFWGWRELSVVIPGWLPQSPGYALLDKNLHFVSFFVESDLYEVPGTFY
                     FYLDNFRVITDLSEFTGDTSIRDNW"
     gene            48979..49809
                     /locus_tag="PLV81_03330"
     CDS             48979..49809
                     /locus_tag="PLV81_03330"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HOM09166.1"
                     /translation="MKYTRFIALCVVIASVVFVGVFPEKAQSRIVTNVQPDISAEQYN
                     ALVVEDFETQSDWVLETVPKKHQDPKKDPVPVLEMKYVDGGPADLIPEKWSPLNKGLE
                     KKKCLGIHFKFKYPGFNSVHILPPLEVRWDDPTQKVLTYDSRAGQEVQERAIQLPGRA
                     KAVSVWVHGRGNNYDLEVWIKDYKGDVHILKMGSLNFVGWRPMKAYIPENVPQETNSY
                     PQTRVTKIVRFVVRAKPEAGNEDVYLFFDQIKVLTDTFEVNFDGQNLHQLMEKGGGTK
                     "
     gene            50137..52833
                     /gene="greA"
                     /locus_tag="PLV81_03335"
     CDS             50137..52833
                     /gene="greA"
                     /locus_tag="PLV81_03335"
                     /inference="COORDINATES: protein motif:HMM:NF011309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOM09167.1"
                     /translation="MEKEKLIERVNSMFKEEMWGRLDPKDVGISRFRILDDLFNSIVA
                     AGIVKEVRDICKEHIQSHPDSIVASYLIGLTGYHLDIIDDKMEMRRLIDMYQKYHKWV
                     IVELLSEKVLEYGESSFALKSLATSLERLGRSKEAIPVWENLLKIDRFDAEVAKKLAV
                     ALIDEDKPKSIQYMKLSIEGHIKNGLYDEIADLWNKLVQVSWEDIQFFERIERMLIEA
                     RQYELTASLLKVLLHKYKDEEHPDQSIQILKKILEYTPNDTAARRELVKLYEKKYGSH
                     SQFQLFLKLSKLNNFKYPVKHAIQDFENSIIFDKGHYVRHRSWGVGRIVDITPNNIII
                     DFKEKPGHQMSVQMALQALTPLKSDHIQVLKYEDYEGLKKIFEEDFLQFFEILIRSYN
                     GSIALADVKKELIPDFVSEKAWAKWWSNKRTEIKKSPLFGISPEKKDVIIMRDKPLTF
                     ADELLDNFLKTDSFTEKLDVVIEFVNNIDAGEGVAVAQFFVDYFSGELKGDSVTRKIL
                     SYFILNGLSKFIDAKKLKLDQAYKGIVAIVKESQDLPSISRKIGSYDYKKDLINLIKE
                     AREDWADIYSQILFETPVRIHKYIVNILIRSHEYGIINRFVEKTLIGAKEFPEIFLWV
                     ARNLLTKSWDYEWLDYSREQLVLTLFRLMNELKKIEQKGSKLKNIALDIIFDNDAAVL
                     KDIVNTFDIQFLGKVYDIVGSIPYIEDSMRDKFFEIIKSRYPDFKPLMKTAEEEWKVD
                     AEVLITTQEGYVRKQEELNRLVNVELAAISKELAKVSDATGDVRENVEYNALLEKQSI
                     LKRDINKLESEIKKAQILDLNTVSTDKVNIGTVVTISGDGEKETFAILGPWDADFEKR
                     ILSYRSPIAQALLGKKQGDVVEIRKNEQIKQYTIESIEKYKP"
     gene            52830..>53705
                     /gene="lysA"
                     /locus_tag="PLV81_03340"
     CDS             52830..>53705
                     /gene="lysA"
                     /locus_tag="PLV81_03340"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOM09168.1"
                     /translation="MNSFREYFTYRNGKLYCEDVSLEEIAEEYGTPVYVYSSKCFMDK
                     FTQLDKALSSHPHLICYSVKSNSNINILQMMQSAGCGMDVVSGGEIYRAIAAGVQPEK
                     IVYAGVGKTTAEIELALTTGIRMFNCESLPEVERINAIAQRMKKTAHIAIRVNPDVEA
                     NTHHYITTGKKENKFGISLQLLPQIVSAIKNMPHIQLTGLHVHIGSQITDVEPFEEAC
                     NRALEVMNKLKAMGMDKCTTLNLGGGFGIQYNDENSLDIARWAKVIDNAIKDKNIFLI
                     IEPGRYISGNSGALLT"
BASE COUNT        17247 a         9080 c        11149 g        16229 t
ORIGIN      
        1 aaaaggggtc agagcttaaa gatcaagcat acataaccaa tattcagact acaatatttt
       61 ttatcacctt acgtgaaaaa tcagtaagaa tgcgagcagt tagaccccat attgtatatc
      121 cattgaaatg gtaataataa atggcgtacg gtgtgccttc ataggtataa tatttgacat
      181 cgtcatattt ttcttcaata aataaagaaa gaggcacctc agtatatgat tcgatttcat
      241 cgtgattaat ggtaagatca taaggatatg gtataattcc aacaaaagta tatacatgaa
      301 aaccatatat tgatataaaa tggtcaaatt cacccaatat tgtcacatca tctggcttta
      361 tgccaacttc ctcataggtt tcgcgaagcg ctgtatctag tggggttgca tcatcttcat
      421 caattccacc gcctggtaat gaaacctcac ccttgtgagt cccaacgtta tttgtgcgtt
      481 tggtaacaag cacatgaggc tgattgtctt ttaaaagaat aggaatgagc actgcagcat
      541 ctttcagact tttgcgttca attgctcggg gtgggcgctg gtgcaatacc tgctttatcc
      601 tgtcacaaaa tttcaaaaat tcgttataat cactgaactt aagcatataa atcaccgcca
      661 tacatatcat tgattacaat gacaggaaat ttttctactg tcaatttata cattgcttcg
      721 gggccaaact cttcaaacgc aactatctgc gcatccacaa cacattgatt gagataggca
      781 gcagcacctc caaagcttga gcaatatacc gcgttataat taaccaatag ctttttaact
      841 tcactgctgc gagatccctt gcctatgaac atcctgacgc ctaacgacag catggcttca
      901 gtataggcat ccatacggta agaggttgtg gggccgatag ctcctatagg catacccggt
      961 ggtgcaggac ttggccctgc ataatagatt actgcattag taaaatctat ggggagaggt
     1021 ttttgctgtg caacaagctc tgctatcttt ttatgagctt tgtcacgggc agtatagatg
     1081 gtgccagata tagttataaa gtcccctgcc ttcaattgtt gcaaatcgtg ttctggtatg
     1141 ggtgtatgta aatcaatagt tttcatagta tagtggacct gtagcgtaat gaatggcagt
     1201 taagattaag tgcaacggga agtgttgcaa tatgagtagg agcttctttt attctgacag
     1261 ttgcaacagt attgttgcca ccaaacccta aaggaccaac tccagttgta ttgcaccttt
     1321 gtaatataac ctcttcaagc ctgcggtatt cttcgttggg atgtggttct ttttgcaaaa
     1381 ccgccttttt tgcattaagc atggcaacat cagcagttcc accaattcca attcccaaaa
     1441 aaagtggagg gcagggattg ggaccagcct gtttaacagt ggctactata taaccaataa
     1501 tttgttcaat atctgctgtt ggtgtaaaca tccttaatga ggacatgttt tctgatccac
     1561 caccttttat gtatactgta atggcaagag aatccccttc aacaatatcg gtgtggatga
     1621 ataccggtgt attggtgcct gtgttggttc tatcaaatag tggatctttt actatggatg
     1681 gtcgcagata gtgacttctg taggtatctg ccactgccgt atttattatc tcttcagggt
     1741 tgccatcaat ggtaacgccg gtgcctatct gtaaaaatac tatcactgaa ccacagtcct
     1801 gacatagagg aatatgcttg gtagcagcga tggtggcgtt ttgtagcaat atatgaagag
     1861 tttctttggc taaaggtttt ttttcactac tgtgcatggt agatagtgcc tgataaacgt
     1921 ggtcaggtag gttaaaatta gcctggacta caaggtttga aatagcagta taaagctctt
     1981 cgtgagaaat ggtgcgcatt tacttttgtt cttgttccag caatgttgtg tattcatccc
     2041 acgttgcttc tatctcgcgg gctgcttcgg gatcaagtat ttctatgata aagcctgcat
     2101 ggacaataac tttatcgttg attttaacgt caggggtcag agcaatgtca acaaccttct
     2161 ctactcccat agattcaaca attgctttag taccttcaat tttttttact gtcattggta
     2221 ttgcaagaca catgatatcc tcctacggtt tcaacatatt cgtggcagtt gttcttcaat
     2281 taacacaggc agtattcttt ttccaccaat tttagtttga atatatgttt tcccggaaaa
     2341 ttcgttggta atatgaccaa ttatgcatgc atctctgcct tcaggatgtt ttttcatgat
     2401 ttcaacaatc ccttcagcac aacgtttgtc agcaatcatc acaaccttgc cttcatttgc
     2461 tatatataat gggtcaatgc ctaaaatatc agcaacgcca cgaacttcat cttttactgg
     2521 cagattatct tcaatcagtt tagcaccaaa tggagcgttt ttcgtaattt cattaagtac
     2581 ggaagcaaca ccacctcggg tggcatcgcg cataaatttg atagcgtcag gataatgacg
     2641 tataacatcc tcaatcaggt gattgagtgc agcacaatct gatgaaaggc cttttgaaaa
     2701 tgtgagattg ttgcgcagtg ccataatggt ggtgccatga tcgccaatac ttccattaat
     2761 gataatggta tcgtggggtt tgatttccct tcgctggggt ggatttgtaa acactcctat
     2821 gcctgctgtg tttatataga tacggtcgcc tttgcctttc ggcacaacct tggtatcgcc
     2881 ggttactatc gccactcctg cttttaaggc tgcttctttc atagatacaa gaatacgttt
     2941 gagcgtttca atttcaagtc cctcttccag tatcaatgca catgagataa actgaggggt
     3001 ggctcccata actgccaggt cgttaacagt gccgttaacc gccaggcttc caattgtccc
     3061 accagtaaat tcaattggtg taatagtata gctgtcagtg gtgaaggcaa tgcttgttgt
     3121 agcaaggtta agatgtgctg catcatctaa ttgtatggtt tcattgccaa gagtagattt
     3181 tatgaggtta ttgataagat cattcataag cttgccgcca ctgccgtggc cgggaacaat
     3241 tatatccatt acttcctccc gtatttatag tatgctgaac atgggccctc tgatgaaacc
     3301 atgcatggac caacagcatg ttccggtgaa catgctttac caaatagcgg acaatcagta
     3361 gggagtatca caccacgcaa aagctcgcca cagcgacatc cggggtgttc tttgggtggg
     3421 ggaacatcta cagggaattg tttatatgcg tcaaaatcag catattcttc ttttaagtca
     3481 agaccacttt ctggtattac tcctattccg cgccaggtag catcgccttt agtaaaaacg
     3541 ctatacagta tttcaagggc ttttaggttg ccctcgtcac gcacaacacg ggtgtactgg
     3601 tttagaactt taggcgtgtt gtttttaatg agatcaagca acatcagtgt tccgcggatg
     3661 aggtcatgat cttcaaagcc tgcaaccaca gcaggtttgc cataatcggt tgcaataaaa
     3721 tcccatgcct tttttccaat gatggcactc acatgtccgg gtagtataaa tccatcaatg
     3781 ttaacttcgc cggaatcaag taatactttt acagcgggta ccgtgagttt gttgccgggc
     3841 aatagaaaga aattttttat gttttttttc tttgcttcaa gcacacagac agcctcagca
     3901 ggtgcggtgg tttcaaagcc gatgcttaaa aatataaatt ttttatcagg gtttttttgt
     3961 gccatatcaa cggcatccat gggggagtag acaacctcaa cctgcaatcc ttttgatttt
     4021 tcctttgcaa gtgaagtata tgatgaaggc actttcatca tgtcgccaaa ggttgctata
     4081 acacagttat gctgccagcc tatggctatg gcagtgtcca ggtaatcatt agcggtaacg
     4141 catacagggc agccagggcc gtcaataagc cgtaagcttt gtggaaatat gtctttaacg
     4201 cctgtgcgga aaaattcagt tgtgtgtgtg ccacacactt ccataatggc aacaggctgt
     4261 ggcaataaat actgtgacaa taaagcagct tcttttttaa tatcagacat gaatattctc
     4321 cctggcaata gcaatctgtc ctaaggcaat accgccatca ttgcacggta gtttgtgcgg
     4381 ggtgtatacg tcaaatgatt tttttgcaag tcctgttgtt ataaggtgta gcaggattct
     4441 attgtgaaat acgccgcctg aaagggcaac cttgtttatt gaatagtgtt ctctgagttt
     4501 acatgcagtg gtgatagtga cttcagctat tgccctgtga aatcgcatgg caatagcagc
     4561 aacaggaaca tgtaattcta tcatattgac tatttcttga ataatatggg cagtgtcaat
     4621 aacaagagtc tgctcctgct tgatgggcac ttcaaacact gtagtatctt ttattgcttc
     4681 aagtgctttt tcttctaaaa gcatggcagc ttcggcttca gtgctgatat gatgtgcaac
     4741 tcctaagatg gcagcaacac cgtcaaaaag ccttccaatg ctgcaggttg cagggcagtt
     4801 tatggtttta taaactatct cctttacaaa ttctttttcc tgtttttgtg taaaaaatgt
     4861 ggaatcatca acagatgatg tcataagcaa agacagacct atcttccatg ggtcttttat
     4921 ggcatgctca ccgccaggca aaccaaagta atcaaaatgg gctgcacgga taaactcctt
     4981 tgtgtctgca attaacagtt cacttcccca cagtgtatgg tcggttccgt atccagtgcc
     5041 gtcataggca atgccaataa caggctcttc tatgctatgt tcctggcaca ctgaagcaat
     5101 gtgcgcgtga tgatgctgta ctgcaactgt ttgtagtgtg gtttgctcct gcgcaaacat
     5161 ggtggtgaag taattgggat gcatgtcgtg cgcaatgagt tttggcttaa catcaaagat
     5221 tttacaaaaa tgggcgatag tttttcggta aaggtcattg ccccacggag tggcaaggtc
     5281 ccccagaaac tgacttacga tagcaacgtt gtttcgtgcc agcgcaaagc tgttttttaa
     5341 atcaccgcct gttgcaagga tatgaagcgg tgtttctttg atgaagatag gtgcagggac
     5401 atatccccgt gaacggcgaa caaaatatgg cttttcgtca acaacgtata ctactgagtc
     5461 atcggtgtgc gatagtatct ggcgattgta ggtgacaaag taatcggcaa ttgtgcccaa
     5521 acgctcaaag gcatcatcgt cgttgtatat aatgggctca tcggatatat tggcactggt
     5581 catgacgtat atgctttcat cattgctgga aaaaagcata tgttgaaatg gtgtataggg
     5641 aagcatgacg ccaatatagg tgagtcctgg tgcaacgtat gggcttacta tatctttttg
     5701 taatttcagc agtacaatag ggcgctcctt gcttaacaaa agttcttttt ctttttgagt
     5761 cacaaggcaa aaggtttcta aaaattgtag tgatgatacc atgatggcaa agggtttaaa
     5821 tgggcgccct tttcgcatcc gtaaaagatt aacacagtga tcattggtgg catcgcacgc
     5881 taagtgatat cccccaacgc ttttcagtgc aacaatgtgg tgttttaaga tcgtctgcaa
     5941 ctggctgatt atggcatcaa attgagattg aataacctta ccggaactat cgcacagcac
     6001 tatatgaggg ccacactgtg ggcaggcatt gggctgggtg tgaaacctgc gattgtgggg
     6061 gtttgtatat tcttcataac actgaggaca catggaaaat ggcttcatgg cggtgttatc
     6121 ccggtcatag gggatatcgt caataatgct gaagcgggga ccacagtgcg tgcaggtagt
     6181 aaacgggtaa tgatagcgac ggttatgcac gtcaaaaaag tcttccagac actcatgaca
     6241 gatagccaca tcaggtggta taaatacatc gcgtgtgcct gttgtgttgc tttgcagtat
     6301 agtaaaactt ttaaatccat tgggtggaag tttggtgatt ttgatatcaa taatctgagc
     6361 aagtggagga gggttgtttc gaataatgtc aacgcattgc ttgatagctt cgtcaggtcc
     6421 ttctaaatga attgttacac cttctgtgtc atttgaaacg ctaccgcaaa gattgtggct
     6481 aacagcgaca ttatacacaa atggcctgaa tcccactccc tgaacaatgc cttttacctg
     6541 tatgtgataa tgatgtgtca tactatgcat tgtagcacaa aatattcata gcctgcaatt
     6601 catacgtaaa aaattgatca gcaaaaacct gtgggcatat gaacaacgtg atcctgtggt
     6661 tattccaata aaaaacatgg tctttccagg gaagaatttt gatgtatgca aaattgcttt
     6721 gttaatctac ttatttcagt gacgttgtgc acttcactat agtttgatag gacaaaggaa
     6781 gtcaataatt ttatgtaata ctaaaatatt attgacatta atttctatat gttatatgat
     6841 agcacaattg ataaagtaag tacctgcagg taatggatga ttaatggagc atctggaatg
     6901 ttattttaaa aaattaataa atcaattatt tggttgactt gcaacgcata ataataaaat
     6961 ttatattttt aatggttttt atgcctaatt tataacatgt aacatgataa atctatacaa
     7021 cgtaaggagg taaaatgtga gtaaaattag gttgtttgtg gtttgtttgg gtatacttgt
     7081 cattgcagta attattggtt gctctgaggg aacaaccaat atcaatccca gcccaactga
     7141 tgttgttacg gtaacagata ttaataagtt tattactgaa aaggacaaac tggcagggat
     7201 tgctgcaacc atgaatgcaa tggatcctgt tgcaatgcca cataaaaagc acgaagatgc
     7261 aggtatcaag tgtgtagtgt gccatcataa agctggcaat gatgacagga taaaacagtg
     7321 tgcggaatgc cacaaaggtg caaagggtga ggataccatt catggtctgt gcattaattg
     7381 ccatgctcag tccaaaaaag gccccgtgct ttgtaatgag tgtcatattg ctaaagaata
     7441 aaggcgtatg gctgatagtg caatgaaacg aggtaaaaaa atattaattc ttggcatagg
     7501 aaatctactt caaaaggatg atggcattgg agtacatgtg gtgcagtata tgcagaaaaa
     7561 caatgtcagc ctgcctgcta atgtggaact gctggatggt ggaactgcgg gctttgatct
     7621 cattccatac atgatagatt ttgataaatt gattattgtt gatgcattga aggtccagga
     7681 caggccaggt agcatttaca gatttgatgg caagcattta aaagcacaga cgcctaacgt
     7741 ttcattgcat gaaatgggaa ttgccgaggt gctaaaggtg ttaaaagttc agggttacag
     7801 ccctgaggtg gaagttatag ggattgttcc tgagaatatc aatacactgg atattacgcc
     7861 aagcccttcg gtagcggaat caataccaaa agttgtggat ttgattcttg atagtgttac
     7921 tgcttattaa cctgatacat taggaggttg ttacatgtcg ctaaatagaa gagaattttt
     7981 aaaagtcatg ggtgctacaa cagctgcagt agcattccca agtgtactta ttcagggttg
     8041 caaaagggca ttacagaaag cttcagaacg taccaatgta atctggattc aggcacagtc
     8101 atgttctgga tgctctgttt cacttttgaa taagcttgag cctgatattg caaccgttat
     8161 taccgaatac ataagcctga actatcacca gacattatgc ggtggtacag gtcatgctgc
     8221 catacaggtt cttgaaaatg cggttaagac taaccgtaaa gattttatat taattgttga
     8281 aggttccata cctacaaaat ctgatgaata ctgtaccata ggtgaaatgg atggcagaat
     8341 cattggtgca cgtgaatgga ttgaaaaatt agggaaaaat gcaatagcac tggttgctgt
     8401 tggttcatgt gcaacatttg gtggaatacc tggtgcaaag atacgggcaa caggcgataa
     8461 cccaacagga gcagtttcat tacagaagtt gttccctgat aagaaggtaa ttaatatccc
     8521 cggatgtcca ccacatccag actggatggt aggaacgctg ctgcatgtat tgttaaaagg
     8581 catgcctgaa cttgatgaat ataacagacc actcatgtat tttggcacca ctgttcatga
     8641 aaagtgcgaa cgacttcccg attataaacg tggacgtttt gcaaaacact ggggcgaaga
     8701 aggctgtttg tatttattag gttgtttggg tatggatacg ggatgcgata tccccacccg
     8761 aaaatgggta ggtggtatga atacctgtac tggctgtggt tcaggatgta taggatgtac
     8821 agagcctcca ttcccagatt atggcaacag aggtatttat aaacatcttg tagcaagcaa
     8881 tgatgaatta caaacgctac atcctgaaat tgccagcact gtagtaaaac ttaagaatgg
     8941 aggtgtcatc aatggctaag gtattattgg atcccattac cagaatagaa ggtcatcttt
     9001 ctattgagat agatgttcag aatggcaggg tcgttgatgc aaaaagtaaa ggtgatatgt
     9061 tccgtggttt tgagatgatt ttaaaaggtc gaaaccctgt tgatgcaaat cagatcacac
     9121 agcgtatatg tggcgtgtgc cctgtttccc acggtattgc atcaagcaaa tgtttagatg
     9181 cagcgtttgg tatagtgccc aataaaaatg gacgactgct cagaaactta gtatttggtg
     9241 ccaactatct gcagtcacac atattgcatt tttaccattt agctgcactt gactatgtgg
     9301 atataactgc tatcctgaaa tacaatggct ctgacaaaaa gcttcttgcg ttgcgtgatt
     9361 gggccaaaaa tgagcttgag gtaaagaaag gccgacagga tgctattaca gctgtagcac
     9421 cgtttttacc acgctatgaa ggcgattttt acattaaaga tcttgatttg aatattgcag
     9481 cgatagctca ttatgtacag gcccttgata tgagaatgaa agctcatcag atggttgctc
     9541 tgtttggagg aagggtgccg catcttattg gtttagtacc aggcggtgta acgcaggtgc
     9601 ccacacgcag tgctatacgt cagtacagaa aacttttaaa acaggttgaa gaatttgtaa
     9661 atgaagtgta tatcaatgat gtaatagctg tggcaaaagc atacagtgac tatttcaaga
     9721 ttggcaggta tacaaacttc ctgtcgtatg gtgtatttga agaaaatcca gagcagacgg
     9781 agttcttctt taaacgtgga gtttttgatg gcaaaaaact acaagaattt gattcatcaa
     9841 agattcgtga acaggtacgg tttgctcgtt acaactctgg cagcaacctg catccatacc
     9901 agggtcagac taatcctgat ccacacaaag ctggtgcata tacatggctt aaggcaccac
     9961 gatatgatgg catgccaatg gaagttgggc cattagcacg cgtagttgtg aactatctgg
    10021 gtggcaatgc agacgtaaag aaagaagttg atgcattgct caaagtgttc aatgctgata
    10081 tcgatgcagt attctctaca ttgggacgtc atgctgcccg tgctatagaa tgtaaacttc
    10141 tttgccagag aatgtatgag tggctggatg atctggaagt tggcgaaatg ccacgctcaa
    10201 cgtatgaaat accggacaaa ggccagggtg aaggcttgac tgaagcacca cgtggtgcat
    10261 tgggacactg gattgttgtt gaagataaga agatagcaaa ttatcagtgc gttgtaccaa
    10321 caacatggtt ctgcggtcca cggggtgatg atggtaagtt aggaccagtt gaacaggcgt
    10381 tattgggtac accaataact gatcccagga atccaattga agcagcacgt gtagttcgtt
    10441 catttgaccc atgtatagcc tgtgcggtac atgttgttga aggggataag gagataggaa
    10501 ccttcaagat ttgctaatat tgtgaaattg aacaggataa taaagggcta tcatcttata
    10561 aaggtggtag cctttttttg ttcaaatata ataagatatt tatagtaaaa gtatttgaca
    10621 gatacaaatt ttattatatt ttgtatttat aaaatatagg aagtgattat gagcattatc
    10681 cgagatgtta cccttgaagc actaaactat atccataatg atgatatttt gatttttatt
    10741 ggagctcggc aaaccgggaa aacaactatt ctacggcaga tccagaaatc tcttgaaaaa
    10801 gaaagcactg cagtttattt tataaatctg gaagatccag attatttaag tttacttaat
    10861 gaaaatccca aaaatctttt gaaactgttt agttttgatt tgaataaaaa aagttatgtt
    10921 tttatagatg aaattcaata tcttactaat ccatcaaatt ttttaaaata tttttatgat
    10981 gaatataagg gaaaaataaa attatgtgta tcaggttcaa gtgcttttta cattgataaa
    11041 aaatttaatg attcgttggt tggacgaaaa aagttgtttt atgtgcgcac attatctttt
    11101 aaagaattcc tacgctttaa aaatgagaat aaactgatag ataatgattt taaaaatctg
    11161 agtataaccg aaaaggaaaa aatatcattt tattatcatg agtatatgct atatggtgga
    11221 tatccaaaag ttgttttgag tgattttgat caaaaaatag atcttttaat ggatatagcg
    11281 tattcatatg taaaaaaaga tatatatgaa gcacatataa aaagtaatga agttttttac
    11341 aagttatgta aaatattagc atcgcaaatt ggtggattgg taaatacatc agagttagca
    11401 aatactctag gtatttcaaa gacagcaatt gataattata tatatatatt acaaaagtca
    11461 tttcatatat ccctggtaag acctttttat atgaatgtac gaaaagaatt gaccaaaatg
    11521 cccaagtgtt attttatgga tactggactg agaaattttt ttgtttcttc ttttgagtct
    11581 tttaaaacaa gaaaggataa gggacagcta cttgaaaatg cgtgttttcg acaactggtg
    11641 gatagtaaca atgaagataa tataaagttc tggcgaacta tacagggtaa tgaagttgat
    11701 tttattgtta atgagaaaat agcctatgaa gtgaaggtag atgaaaatca gtttagaaag
    11761 agcaaatact ctgtttttat gcaaatgtat aaatccatac caatagcgat agttacttta
    11821 gataaaaagg tagaaatggt tgagggttgc caggtttttg atgtgtggga aatataattt
    11881 ttttatttga aaaaaaatgg tttatttaat tattgaaagt aactttaaac gaacactata
    11941 attttgttgc tttgattatt attcaaatta taggagaata acaatggaaa tccaggtatt
    12001 acagaacatc tatgaatcaa atattcagaa ggctgaggaa ataaaaaagt tactcagcca
    12061 aaaaaaagta tatatgtttg acatcatgag ttcacccggt gcggggaaaa ccacaattac
    12121 tgatagaata atcaatgacc ttataaaaga ctacaatata gccctcattg aaggcgatat
    12181 cgaaacaacc aatgatgctc agcgtttggc aaagtataat attcccattg ttcagataga
    12241 aacaaagcta tttggcggtg cctgccattt agaaagctcc tggatattgg ggtgcctgga
    12301 aaaatttgat cttaatgctg ttgatgtggt cattattgaa aatgttggta atctggtatg
    12361 cccagccgag tttgaattgg gtgatgatga acgagtggtg gtattgagtg taacagaagg
    12421 tgaagacaaa ccggttaaat acccgttaat gtttaactct tcacatacgt tattgattaa
    12481 taaaatagac ctgttgccct atctggattt caatatggat gaattactgg caaatgttaa
    12541 acgcacaaat ccaaagatgg aagtacataa agtaagcgca aaaaccggtg aaggaataga
    12601 aggattttta aaagcactgc gctcgcggat aaaaaataag gttggcaggt aacagtataa
    12661 taacataata atgtacggga catcatttct actttcggca gcgcttacgg atgctttact
    12721 ttgacatgat gtactgtgca cgtggagtac caatagatga ctaaaactct attatgggtt
    12781 ggttttaatg tttttgtcct gcttatgctt cttttagatt taggtgtctt taacaggaag
    12841 gctcatgaaa taaaagtaaa agaagcggta ctgtggacaa tattctggat agtgttgtct
    12901 ttgatgttta atcttggcat atatttttat gccggatatc ataaagcttt gctttttttt
    12961 actgcctacc ttgttgaaaa atcattaagt atggataata tatttgtatt cctgatgctt
    13021 tttacatatt ttaaagtaca tccaatgtat cagcataaag tacttttctg gggtgtactg
    13081 ggtgcactca ttatgcgtgc catttttatt tttgcaggca tagcattaat agaaaagttt
    13141 gactggatac tgtatatctt tggtgcgttt ttaatctata ctggatacaa gatgatgaaa
    13201 gaaaaggata aagaaattca tcctgaaaaa aatccagtgt taaaattagc acgtgccatg
    13261 ctgcctgtta ccagtgacta tcgtaatgga tatttttttg ttaaagaaaa tgggaaacga
    13321 tttgtaacac cgctttttat tgtcttgctg gtcattgaat caacagatgt attgtttgcg
    13381 gttgattcaa ttcctgctgt gctggcaata agccatgatc cgtttattgt gtatacatcc
    13441 aatgtcatgg ctattctggg actaagggca ctttattttg cgctggcagc agtcatgcgt
    13501 ctttttcgtt ttttgcatta tgggttatca gttattttaa tctatgtagg ctgcaagatg
    13561 attgctgctc aggttgggta tcacatccca acggaaattt cactggggat aattgcattt
    13621 gtattgacag catcaattgt aacatcaatc tatattcctg aacataaata aaaaaaaata
    13681 aaatattgac agaggcataa caaaaagaca ataccagttt tcctgtggag gtagggttat
    13741 gataaccgtg ggtattaata ttggttcaac cagtgttaaa gttgtggtgt gtaacagtgg
    13801 tgttgttgaa acagttaata tcatgcccca tgaaggtgat gtgccgggca cgcttacaac
    13861 aattctatcg caattaaaca ttcccaaaga tgcacgggcg atagttaccg ggaatgaagg
    13921 aaggttttta ttacatttac cccgtgtcat agaatctgtt tgcattgagg aagcgctgaa
    13981 acatagtgat tacaggccag atgcaatagt atctatggga ggagaggacc ttgtagtcta
    14041 tactgttgat gaacataaaa aaattaagaa taatttttca gggaacaagt gtgctgcggg
    14101 taccggtgag ttcttcaagc aacaattggc acgcatgaac atgaagcttg ctgatattca
    14161 ttccatccca catgaaagca aggtattgcg gttatcaacg cgctgttcag tatttatgaa
    14221 aagtgactgt acccacaggc tcaataaagg agaggcaaca aaaggtgata ttattctttc
    14281 actttccaat gtgatggcag ttaaagttgt agatttctta aaaaaagcac gtatacacaa
    14341 tggtacagtg attataactg gtggcattac ccgtaatcca tatatagtac agtttattaa
    14401 agaaaaattg cctgatattg attttatcat accagatact gctgcatatt ttgaagccta
    14461 tggtgcagca ctgctggcac agcagtatgg ggaacctatt ggtgatactc tatttgaaaa
    14521 acataccata agctttcctc ggtttaacag tttaaaaacg gcacttaata atgttacata
    14581 tattccctca aagcgtggtg ttgcaaaacc tggtggagag tatatattgg gtgttgatgg
    14641 tggatccacc acaacaaaag catgccttgt tgatatacac actcatgaga tagtagcagc
    14701 acactatgga cgaacccatg gcgaccccgt taaggcattg aagcagtgta tagaagaaat
    14761 tcaaaagcag ttaaaagatc agataggtga taatcccatc accatttcgc ttgcagcaac
    14821 taccggttca tcacgtgaaa tattgggagt ctttcttgaa acgtcggcaa tctacaatga
    14881 aatcattgca cattctgttg gtactaccta ctttaagcca gacattgaca caatatttga
    14941 gataggtggt caggacgcaa agtatgtata tttgaaaaat ggtgtaccca ttgattatgc
    15001 catgaatgaa gcctgctcgg cgggtaccgg ttcgtttttg gaagaatcag ctcagggcga
    15061 tctgaatatt gcccatgcat gggaaatagg cgatatagca ttacaggcag aacaaccact
    15121 aaaatttgga gaacattgtt cagcctttat taattctgat atacgcaagg ctattcagca
    15181 gggagcaagc agggaggaca ttactgcagg tcttattaca tcaatagtgg ctaactatct
    15241 caaccgtgtt gtgggaaaca ggactattgg aaattccatt gtgttgcagg gaggagtagc
    15301 aaaaaacaag gcagtaccgc ttgcatttgc catgcttctt ggaaaacaca tcacagtgcc
    15361 gcctgacccg gaactcatgg gttgttttgg tgtggcgata gtagctctga ataagttgca
    15421 cgatggcctc ttacagaaaa aattctattc gcttgatgca attcttaata cagacattgt
    15481 ctatgagcgg gagtttacat gcaaggcttg cgataactat tgccccatac gggtactcaa
    15541 tgtcaatggc aacaggtaca tgtttggcgg cagatgcaat aaatatgcaa atatgcggaa
    15601 aaaacgcgtt atccatgaag aggaggttgt tgattttatt gaaaaaagaa atgagctatt
    15661 gttccatgaa tgcgcaccac aggaaatgcc acacaatgcc tctataacgg ttggtgtacc
    15721 gctgtgtttt tctgcatata ccttatggcc gctgtattca tggtttttta aagaattagg
    15781 ggtggcgata gttacctctg atgcaatctc agaagaggga aaagctaagg ttgaaagtgc
    15841 ctattgcttc cctgctgaga tagctcacgg cgctgttaaa aatatttttg ataaacatgt
    15901 tgattatatc ttcctgccac acagtaaaga aatgaagagc tatcagaatg atgcccgtgc
    15961 aacgttttgc cccattactc aagggttacc atattatatt gaaaaggcat ttgatgaaat
    16021 tcctaaagaa aaaatacttt caccagttat tagctttaaa ctatcagata caattactga
    16081 agattcattt gtaaatgttg ctgtaaaact tggattttca tctgaagaag ggaaaagagc
    16141 attcaggctt gcattacaaa agcaacatga atattttgta aaagccagaa aattggggga
    16201 agaggcgtta gagtttgcac ggaaactcaa taaacctgcc atagcattac tggggcgacc
    16261 atataatgca ttcacaaaag atgcaaacat gggcatacca aaaaaatata ccagtattgg
    16321 atatacggtt attccgtttg atattcttcc ctttgaagat gaggaaatat ttcctaacat
    16381 gtactggttt tatggtcagc tggatatgaa ggcaagcgtc atgcttgcaa aagaaaataa
    16441 tatttatgta acatttattt caaacttttc atgtgcacct gattcattca tgttacacta
    16501 tctgaaatgg attatgggtt caaagccatt tcttatcctg gagcttgact cacatacggc
    16561 tgatgccggg atagatacac gcattgaagc tttcctggat attattgaag gctacagaca
    16621 aaaatgtatg ggtgaagaaa cccagcgcta tgataatggc ctgcgctttg tcattgaaga
    16681 taatgaattg tacgtacgca atatagttac taatagtaag ataccagttc gcaataacaa
    16741 aaaggttaca ttacttttag caagcatggg gcgtttaggc gcagaacttt tagcagcgtc
    16801 acttcgttcg gctgggatta atgctgaggc aatgccagtg cctgatgcta aaacattgca
    16861 actggctcga caatttgtgt caggtaagga gtgcctgcct tcacatctgg tactgggaag
    16921 tgcattgcag tactttgctt caccacgata taaaaaagat gaaatatatc ttttatttgt
    16981 gccaacaaca cagggaccgt gccgaacagg gcagtacttt gttttttatg aaaatctttt
    17041 taaggacatg cgttttgata atgtatgtgt agtaactttg gattcatcac attcctatac
    17101 agaactgggg ccacacttct caaagcatgc atggtgggga gttgtcatca gtgattatat
    17161 gaaggacatc gaaacgtcgc tcagagcatg tgccaaggat cccgtaagcg caatggctct
    17221 gtatgaccag ttgtggcagg agttaatctc agtagctgaa aaggatgtga ccagagttat
    17281 ccccgtgtta aagaaaattg cgcaaactat cagcaatatc ccattacaga gaactatcgc
    17341 acaatgccct aaagtactga ttgttggcga gatttatgtc cgcagggatg attttgctgt
    17401 tgatgaactt gttaagcact tcagtaaaaa gggaatcata gcaaaagttt caggagtcag
    17461 cgaatggatc tattactgtg atttcatacg tgagtatgaa tataaaaagg ccattaaact
    17521 ggcaccgtgg tataaaaagc tgtttatccc tgaggtatgg tcgctgttga gattttttat
    17581 tgagcagtgg tacaaacacc gtgtggagaa aaaagttaaa caggcgttac aacgtacaca
    17641 gcttatcccg gataccccgc atgatatgca ccacatcatg gatatgtcac aaaaacattt
    17701 tgtggatctg gaactgcaat ctgagattac tgtttcaagt ggtgtagcag caacagcaat
    17761 gcaggatggc tattcaggca ttgtcaatat atcaccgttt gcctgtttaa ttggtcgagt
    17821 tattgaaggt attcttacgc cgtgggctcg aatacagggc tatcctgtta tgtcagttga
    17881 aattgatggc aatgccctgc cgccaaccac gctgaataaa cttgaaatat tcatgttaaa
    17941 cgttatgcga tttaaggaaa agccagaaat gaaggaagtc gttgaacatc ctgcacaaac
    18001 ggcagttgca attgacagga aaataattaa ggatttataa tacactaagc ttttttggta
    18061 aaagctattg cctcatcaac agtgtgaaat actgcaaaaa aatcaaacat tgcaggttgt
    18121 tcaatcatta ttttttgcag cagatcatcc gtcattacca gtaatgcata accacgtgtc
    18181 tttttccaga attgcagtgc tttcattaac acaccaataa taggattgga aacctcctgc
    18241 accgataaca catccagaat aaaatgctga tggtcaggat ggttttgtat atcttgcttg
    18301 acaaattcta agatttgatt agcctcttct ttggtgattg caggcttttt gatcttccca
    18361 atgagaatag cattatcttt taaaaattca gaagaaaaaa gatcatttaa tgtactcata
    18421 gtttaccctt atgtataaat atttatgtca tgatacaaag ccaataaatg atatgtcaat
    18481 gaaaaaaagt tttactatat tggttaacag tgtagtgatt atgctttata ctggagaatg
    18541 tggagcaatc gtttgaaaac attcatacat tattgtatta agattctttc atatagtgtt
    18601 atagcactat gtagccttgt tgtttttgta atgctgattc tttttattgc caatcttgtg
    18661 ttaaccactg atagggtatc aaaaattatt acagatgaag ttgagtccaa tctcaatgca
    18721 aaagtttcat gtcatgtaac acatttttca ttgttcaatg gctttgaaat aaaaaatttt
    18781 atacttacag gtagtgatag ttcaaaacct gtagtaacct ttgatacatt tcggttgcgt
    18841 tatagttttt tcccattgtt aataggtaaa atcaaaatat atgaagttgg attgtataac
    18901 ccctctgtat atcttgaaga gcagaaaggg atatggaaca ttcaaaaatt gagtaaacct
    18961 tctgaaaaaa aagaaaagga agataaggaa gaagctaagg aggaaaagga aaaaaaggat
    19021 gagattacat tgcctattcc agtagaagtg ctatttggat tagtactgga aaatctctcg
    19081 gtaaccattg acagtagcac atatactgca gcgctaaaaa atttttcagg ctctatacaa
    19141 ctgcatatcc ccccggcgcg tcgcatccca ttaaatatac tggcattagc attatttgac
    19201 aaattaaaga ttactgttaa tccagaagaa acgttatccc tttcgtttgt ttccagggat
    19261 attactgccg gcccaccatt gctgtttaca tttaatctgg tatatcatcc cgaagggaat
    19321 cagttattca gtagctttaa gtgtggtaca tataatacgc ctgtcagatt tgcacgaaaa
    19381 catgtagctc cactcaatgc aaagattgaa tatgatatag catacaattc atctgatgat
    19441 gctgttaact gtaaaatatt gcggattgta tttggtaaat caacctggat tacatttgca
    19501 ggtactatcg aacatgtaaa cagtaaacca gagatacagt ttgctatgct tgacagcaat
    19561 atcaggctta acgaaattta tgagtatacc aatgttcttc ttgggccaaa accgtacttt
    19621 aacggttcaa tttcaatttt tcctcttaca atacagggga atatacatgc attaactgtg
    19681 aaaggtgcaa ttcgcgggca aaaggttgca tttgctaccg atgggtttgc tgttactatc
    19741 ccctcagttg agataccata tacggttgtg ctgcaaaatt ctgtcattga tgcatccatc
    19801 aatattgggc ttccacattt tatttataca cttgatgaaa acaggtcgcg tgataatggt
    19861 atcagcttac agtgcaacgt agtatataat cttaatacca gtatgctgga tctccaaaga
    19921 atagttctga tacacagggc tcctgagata gcagcggaga ctatgagggt tgaggtttcc
    19981 ggttcagtga ttttgggtga tattgtggca gcacgtataa atatgtcaac agtacggttt
    20041 gatcttccat caatggcgta taccatgccc gcaccgttga aacagtcgct gacatcaagc
    20101 cccataacaa aacctgtgac agtaagactt aaagcagatg taagtacttc tgcacaaaca
    20161 aatagtatca atcttagagc tcttggtgta attccggatt attctattga tgatttgact
    20221 atttcagcat cagttacaca gaagccacaa aaagggaaga ttgacatagc atactgtgat
    20281 atagcaagca caaagcatca agccagtata tatatgaaag gatcagtgat taccaaagaa
    20341 gatgtgtatc ttgatgttga tgcaggagta catgtagctc cacgggcacc gatttctttt
    20401 ggcaactata cagtaagtgg cgttatgaag atagtattaa gtcttcaggg gactatgcaa
    20461 tcattggttg ctaaagggag tttgcaatcg caacagttaa tactcagtaa tggtgctggt
    20521 aaactttttg ttggcccagt tgatatggat ataccaatag catatacaat ggggcaaagt
    20581 tttaagatgc cttttgattc ggctgatgtt atgaatattc agctgtttaa gcaaaaggca
    20641 aatttttcca ttggtaaaat tatggcacca catccatcgc gaaatattgc ttttgaatat
    20701 gttaagaata tgcatgggca tattgggttc aaacgtaata taatggaaat taaaaatttg
    20761 caggctgaag ttttacgtgg gacggttact cttaaggaat taaattttaa tctggctgat
    20821 cttaatccgc aacacatgga attccgggtg gcgctcaatg caaatgatat tgatattgga
    20881 aagctggata aacctgaatc agtgaggatt aataaagatg cactgttatc acttaatgcg
    20941 caggttgtgg gcaaaaacct caacattgca caggattttg atatatctgg ttccattaac
    21001 atatataagg ttggagagga ttttgcaaac aggcttttta agggaataaa tgaagaacgg
    21061 ggaaagtcaa aattagggat gggacagttt gcagtggata attcactcat tattaccggt
    21121 tttgattttt atgtggataa aggtctggtc tacccaacag tttttttcaa aaagaagatg
    21181 ctgggaattt ttgttactgt taataatgaa caggtgcgct atgagcgtat accggttgtg
    21241 gaatttttta acagggtaag ggaggaaaca ttatgaaaca tatagtggcg atagctcttg
    21301 ctatcgttgc cggtatagta tattcacagg cacgatgtag ttcttcaagt tgcctgaaag
    21361 gagtaccaag ctgtattgaa atacaacccc ctgcaataca tattactggc gagaagacag
    21421 cgctggagcg acagcttata ggcgaatata gagaaattga aaaagatgca tgggttacat
    21481 ctaccgttat ggtgccatca aaaggggagc gccgcgcaat ggttgctgta gatgaaacta
    21541 tgctgcaggc aattaagttg cgggaactca atgaggataa aatacgaaaa tataaagata
    21601 tgggaatcat aggtgaaaat aataatgggt ttgtgggaat agtagatgaa aagaaattac
    21661 agtccatgaa agaaagcaca gtaatacgta cactggttaa tgatgagaac aatgcacgac
    21721 agattatctt tataaaaagt tcagattcaa aagatgccag agaccctaaa gctattcagt
    21781 ttggctatga ttttgctaag gagcagcatg caaaagcaaa aaagagtgac tggatacaga
    21841 aagaagatgg tcgctgggtg aaaaaatgaa acgtatagct tgtgcacttt ttggattact
    21901 tattacaggg tgtgcatcaa cagcataccg cgatataccg tttgattatt cacaggtttt
    21961 gaccaaagaa tttcgttttg gcacaggccg ctcaattcca cttacctttg agagatcagt
    22021 aaatgtgagt ggaagtataa catctgatgg taaatatttt atctatgctt ctaataaaga
    22081 acgaaataat tttgacatct atttgcgagc actgaatgac atcaaaactg tacgtttgac
    22141 aatccatcct gccagagatt ttgcacctga tgtgtcgccc gatggcaagt ggtgtgtatt
    22201 tgtttccacg cgcgatgatc ctgaaggtga tatctttatc atgaagattg atcctgaaaa
    22261 gattgtcacg cagggtgacc aggaagtacc atcaaacaat atcactgcaa tacgggatcc
    22321 cctttcagga gctatcacac cagtgcgtga tgcttctccc tgtttttcgc ctgatggtaa
    22381 attaattgca ttttcttcta cacgtgatgg tgaagaatcc atttacatta tggaaaggga
    22441 tgggactgcc attcgcaaaa ttactgaggg gatgcaacca cggttttcca gcgacggcaa
    22501 agagctcatc tttgtaaaaa aaactaatac tggaagcgta gtatttacta ttaatattga
    22561 tactggcacg gtgacacaaa taaccagtgg caagtacctt gatctatcgc cctgctttgg
    22621 gaaaaccagt aatgacatat attttatgcg tattcagtat gataccaatg gtgatagcat
    22681 actggattct aatgataaag gcatgctatt tgcctatgat gtacaaaaca aagaggagta
    22741 tcccctgacg ttgattgaag agccttcagc cacaccacgc tgggtgccgt atgctgatgg
    22801 tataattgtg tacagcattg ttgaaggcca gtacattaat attgcaatga ttccgcatac
    22861 aggcattatc ccaaaacaga agaatgcttt gaggcagtat gaactggcac tgaagtataa
    22921 agaagatttt gacgacattg accgttatca gcgctgcctg tttgccacat attatttttt
    22981 taatgaatcc agtgatgttg attctcagat tattattgcc agagcactgt atgaattatc
    23041 gcacgtccaa cagtattcat ttgttattac actattatct gatctgggta aacgaagtaa
    23101 acctgcacaa atttatatgc actatcgtac cgtgaaagat agagaaaagc taccatatct
    23161 tgagcaggtt gttaaggaac tatcgacgca aaacgaagaa aaaattgcac cattttttat
    23221 ggaagagctt gctgaccagt atgctggctt acagatgcaa gcaaaagcca aagtattata
    23281 taatgagatt attgcacgat atcaaaatta tactcggtta atgtatgtac ggtataaagc
    23341 aggggagcag ctgtataagg aattcagtat tccacaagaa tggatcactg tgcttcagtc
    23401 ttcttataca taccttaaaa atgcaattac actttcaata attgaacaaa caaaaaaaat
    23461 cccatctaaa gtgcggtatg aacatgcgct aaaatcagct gaaacgtaca acgtgcctgt
    23521 cattaaagca attatgaact atgtggcggc aacagccctt atggaacttt cacagaatga
    23581 tgatgcaata gcattactga aacagggcct ggagtacaca aaaaagctgg atgtggcgta
    23641 ctttttgatt aatgtgacat taggaaaact atattatgaa aaacaaagtg atgagtgggt
    23701 tgtgtactat gaagctgctg tcaacaatta ccaggcgcag tggaagcagg atatacgacc
    23761 tattattgaa cagatggcag aatattttga aagtacaggg caaaggctca gcgctgaagg
    23821 aaacttcaaa gaggcagcgg cagtgtataa acgctcaata accatgaata cattgctgca
    23881 tcttaaacgc cgttataaag atatatatag tgcaaacgca ccaggtgctc acacgggata
    23941 tattaatgca atgctggcaa tgtcacaagc tactgccgag cattctattg aaaaagaata
    24001 cttggaacgg ttgcctattg cacggatgga ttttgataag gcacatatat atgggttagg
    24061 atatatatat gtaaaacagg ctaaagcgct ggctccttca cctgatgcta tggatggtag
    24121 gtcttcacag gaatttgaaa cgattctgca attgttcacc aacgctctgc gacacattga
    24181 ctggtctctg tttattgatg atacgtttgt tgatgcatat ttgctcaaag gatggatata
    24241 tcagtacgtt gatgaattgc gtgtccgttt cccttcaaaa aagcgtttaa ttgattcata
    24301 ttttcctgag tatttatggg aaaagtccat tcccctgtat gaaaaggcac tggcagccaa
    24361 cagcgaaaac ctgttccccc agaaagaggc agatattcat tgcaatattg gaaatatcta
    24421 ttttctttta aaaaatttta atttagcttc ccgccactat gctgaggcaa ttaaatatac
    24481 aaagactttt tcaacattga aagaagaagc tcttttttac tatcactatg gctattgttt
    24541 atggcagcag gggaattaca gtggggcgta tgtgtcaatg cagaaggcgt ttactattta
    24601 tacggcgata gctcaacaga ataagtctgc aattgcggat aagctcttgg tgctctacaa
    24661 gtattttgct ctctttgaac ggatgcaggg taattatgaa aaagccattg agtggtataa
    24721 gaaaacatta gaacattgtg cacaatttca atgtggggat agctcatcac gtctttatat
    24781 agacatagct gaatgttatc gcagattgga acaggattct gaagcgcttg tatatctgga
    24841 acttgctgaa cggtcattgc cgcaggaaaa gtcagtgcag tataaaattg gctggaaatt
    24901 tgctggattt ggccccgtat acttctatga ccttggacag gataccgttg ttattgggga
    24961 ggggaaaatt gtttcccatc tttcaggttt tgagatggaa atacttatcc agaatatgcg
    25021 tgcagatatc tatgcaaaaa gcggcaggtt tggtgatgct gcaaccaaat atctggattt
    25081 agtagcaata acaaaaaagc aaaagactac attaatagct gatgcgcata ttaccgcttt
    25141 gtataatgcg gcatattgtt atacccgtat gggggatgta cagaaggcac tggaattata
    25201 tggcactgct ctatcaattg ctcaggattc aaatatcccc attgataaaa tatatcaaac
    25261 catggttcaa tgggcttact gttacactgc cagccacgaa caaaaagcaa aacaagtgga
    25321 aagccttgat aaggcaatac ggacaacaga gcagtacaaa aaaaatatgt atgataaagt
    25381 atataattcc cttcttgatg attataccaa aaagatgaaa gcacaaaaga aaaaaccagt
    25441 tgatgaagat attgcagctt tgaaacagca ggcagctaaa caggttgagg catacagcat
    25501 taaatttagt gcattgactg cactactgta catgcacaaa gcttctcttc tagaacagga
    25561 tataacatct gataaaagta ctgactatta tgttaaagca aatgaaatta ttggctatta
    25621 ttcagatgct gaaaggttgt tttctaaggc aatcaattac tttggaaacg atgcttctca
    25681 tcaggagttt gttattaaat tgctcattaa tagaggaata tgccgaatac attctgggca
    25741 gttcagggat ggatatgatg acatcattag tgctcgattt aaagctaata aaattcaatc
    25801 aaaagaactg gtgtggattg ctgaagcaac gctgtgggat ttagcaaaca catatagtga
    25861 agtaaagcag ctcatagata atcctgatag gcttattgat ggggtattat cacgggtgga
    25921 attgctgccg cctctgtata caatgaagaa agagtttatt cagaaactct atgatacata
    25981 tgctgatgaa cttatacaga aaaagaaata caaagaagct tatgccattc agcagcgcag
    26041 gcaaataatc actctagcat tcagtggact atcaatacca aaattctata aaaaaagcga
    26101 taataccatc tatagcaaat ttttgcgtac atgtttcact atacaggacc ttgaacaaaa
    26161 attaaccagt gcgatagtga atgaagaaga gccagatgtt attgaaaaac taacatctac
    26221 tatttcatat cattataaaa atcttactgg ttacaggtcg gccgcttcaa gatttctatt
    26281 accctatctt ggagtttata ccccaaaagc ttcagaatat accataattc atttctacac
    26341 atttgataat aaactctatg gatggatagt aaataaaaat actattgatt ggttattaat
    26401 ttctgattca ctgaagcctg aaagattgac tgaagatctt aatacattta tacagacgtt
    26461 acatgggaaa gtctatgttg tgctcaacaa tacattcatt gcactgcata aatatataaa
    26521 caatacagtg ttatatacaa tggggtgtgt gccgtttgct tcacgagtgg atggtattga
    26581 acgcctggtc tggttgccag cagacgtagt tactacagat acaaagcttt ccagtaatat
    26641 acataccact gctaatatta acaattcaga gtatatagtt gatactgaca atccagatat
    26701 tactataaca ccgccttact gctttgctaa caaattggaa tgctctgctg tttttaaatc
    26761 tataaacaac atgaaaactg atgaactcat gcttttagcc gatgcaagct tttattctca
    26821 cattgcatct gttattctgt attctggaga caacgttgag actttgctta cagcagtgat
    26881 aaataatgat ataaaactta ttaattctct gaaaacaaaa acggtggtgc ttggaaaaat
    26941 tccagacaca ataaaagcta gggaacaaaa tgcacagata attgttcagg aaaaacatac
    27001 tgagtatgcc aaagctttag aacgtggcga ttatgattcg gccttgtctg ctttagagcg
    27061 ctggaaaagg gcaggattac aacaacaaga atattatgac aaaaaaatac ttgttctggt
    27121 ttaccataat aacatagatg aagcactcaa agttatgcag tctcacagta gtgcttttac
    27181 cagttcattt tctgtttata catatttgtt aaagggtgat gttacaagcg cggaaaagca
    27241 actgaacaat cctcatctgg caggtacatt tgattatacc gtttatgagt ctatcattaa
    27301 aggctataaa cataaagaaa agcttactgt tattaacaac gtatcaaaaa gtactgcttc
    27361 aattttgcct gctaaccaat tgttgttgct gtatgccgag gaggcttttt tgctgggaga
    27421 aaaggaaaag gccaaacata taatggaatt gtatcaggcg gactatattc cttctcaacg
    27481 ggagttgctg gttgcataca atttgggtta ttcaatagct tcgcatagta atacagttgc
    27541 atacaggatg ctaaaagcta ttactggtaa cagtaatagc agcaatttta taaataccat
    27601 aaacgaatat aataacctgt tgctttttgg aatactggaa attgtcaaaa atagaccact
    27661 aatgcccgat gaagaatggc aacgtatcat gctgattgct gaaaagggca aacacatggg
    27721 gattgatgta caatttacaa ctgcaaaaat agctgaaaca ttgcagggta tcattccagc
    27781 aagagtaatt cagtacatta aaacagttgc tgtagacaag ggaaagattg tatcacctat
    27841 agtaaacctg tatgcaatca atgcctatca aaaaacagat aattttaatg atgcatatac
    27901 tgcatgcaaa accttaaagc aattgccata tacactggag cagttatggt acacctatta
    27961 tatctattca gcaataatgt tagggaaaac tgatgatgca caggagcttt taaacaatag
    28021 tctaatagct acctgggatc ttgttatgta taatgttttc aatgtttgca tcaaactgca
    28081 aaaggttgtg gcattaaaaa ccactgatag caatatgttg caaactatcg cccaaaacat
    28141 tgatgtgttg attaccagcc tgcatgatga atcaatacga tggtattatt ctgagtataa
    28201 atacttattg gataaactta ttaattatgc aatatcgtat gcaatgagca ggggaaatca
    28261 gcgggatgct ctgaagtatg ctgaattaca taagatgata gaaggaattg cattgtttga
    28321 tgaaaacagc agctataaaa gtatgtataa tgatcttatt ggaaataaat ttccaattat
    28381 ggaaatacag aaaagtattc ctcgcaagac agctgtatta tacacaacaa agaatgaaaa
    28441 tgatatattt gcctgggtta taacaagaac ctatatacag ccagtacgcc tgcagaattc
    28501 ttttaaacaa ttatatgata ttatacatga ctattacaat gatgttggat tgcttcaaca
    28561 ggcatttgat aaggaagcag caattaatac tataatgcaa cctgtaataa aagagctcag
    28621 tggttatact accataataa tcattcctga tgcatatact gaatctatac cgtatgaaaa
    28681 tattcagtat aataaaggtg atacagcaat agtttttatg ccatctctga tggctgctat
    28741 ggacaaaaca tcgtccattc cccccatagc atatattaat gtgaacaata caaaaaagga
    28801 tcttattgta tcagctctaa aacaatctga ctttgcatat acatctgatg caacagtaaa
    28861 aagtatcata atcaccgata gacaaatcag ttataataat gttaaaaaag ggttgatgat
    28921 taatggtgat gtgatacaat cgggaaatgt gttcattaat ttagctaaca catcgcaggt
    28981 gtcgtactat tattgtttgt ttatgataca gaaagaaatg ggaattagtg tactggtgaa
    29041 tagtgctgtt tcagatgtta atagtattat cttcatccgt gaattctgta acacaattaa
    29101 aactgatagt gctttaaatg cttatatatc agcatatcaa tatttgaaga aagacagacg
    29161 ttttaaaaat cctgcctact ggttgggaat tcgtttgtat agccgcacaa taaaaggtat
    29221 atatgatggt gtcaataaat gaaaggacag gacatgccaa cagcggtaat tatcgatgat
    29281 gatgagatgg ttaatgaagt cttaaaattt atacttgaaa atgattataa cgtatataat
    29341 tattacacag cagaggatgc gctgaaaagt gatgtaattg catctgccga tattattata
    29401 acagatgtga acctgccagg cattaatggg ctggaattta ttcataaagt gcatgaaata
    29461 gatgaaaatg tccccatcat cgttatcact gcatataatg atattgaagt tgcaatatca
    29521 gcattaaaag cgggagcttt tgattttatt ttaaagccat tcaaaaatga tcagataacg
    29581 cttgcagtac aaagggcgta cgaacgccat aaacttgtaa aagaaaatat aaaattagta
    29641 gaagaactta aattaaagaa ccaggaattg gaaaagcttt atgcacagtt acaggcacgt
    29701 tccattcaga ttgagaatga gcttgatatt gccagtaacc ttcagcagtg tcttttccct
    29761 gttgtcttcc cagaaattaa aggattttct ttttatctta aatttaagcc tgttgaaaaa
    29821 ataagtggtg atttttttga ttttataatt catgacgaaa agcatttctc attcatattt
    29881 gctgatgttt ctggccatgg ggtgccagct gctctttatt cagcaatcgt aaaaactgct
    29941 ataacaacgt ttgtagatat atcaaagtca ccttctgata tattaaaaga gatgaatcag
    30001 ttcttaatac agacgcaaaa gatgatgagc tacaattatg caactatatt ctttgcacat
    30061 tttgatttgg ctaaatcctg tatcgaatat gtaaatgctg ggctgccttc accaatagtg
    30121 atgcatccgg atggacacat aatcaggatg gaaacaactg gtccctttgt tggcatattt
    30181 gatacttcgt tgtatacaac taaaaaaatt gaattgcttc ctggtgataa attgcttttc
    30241 ttttctgatg gtgcctttga atgtccggac agagttgatc gaatcctggg agaaaaacgt
    30301 tttcaagaat taattgatga gcatagagat cttgaaatac cagaactgat agaatttcta
    30361 tttgataaaa ttcaggattt ttgtggagta gatcgttttg tagatgatgt aaccatgtta
    30421 ggaatgaaat tcagtacaca ttgataaatt attgcacttt tgtgaattaa ttatagtact
    30481 tatatagcat aaagatcaca ctgatttact aatcatgtaa ataaaatgta gctaatgaaa
    30541 tggaggatgg catgagaaaa ttcatgttta tatattgggt gcttattgtg actgtattgt
    30601 tatttgcacc ttatgtatat gcattagaga ttagctttgt ggttggtgat gtcattgttg
    30661 aacgtgatgg aaaggaaaat cttgctgttg ttggaatgaa actgtcacag ggagatgtcg
    30721 ttgttacaaa gaatgcaagt attgcagaat tgctgtattc agataaaagt acgctccgta
    30781 tttttgaaaa tacaaagatt aaggttgcag caatacagga caatcaggag aatgtatctg
    30841 tcattaatgg agttatcaag gcaaagattt caaaattaca gaagggcgag tctaaaaaag
    30901 tgtatactcc aacgactgtt tgtgcaatca gaggaacaga gttctttgtg gctgttagtg
    30961 atagttcaaa ttcacgggta aatatggaag aaggatcgct ggcactggcc aatccttatg
    31021 gagaaataag tgtaaataac gatgagcagg ctactgttga agtagcaaaa aagccgttgc
    31081 tggatgataa ccctaaaaat cctgaattgt ggaaaaacac agcagataaa gattttgtcc
    31141 tggagccgca aaaatatgca gaaagctata atgaatattt gtctacgtta gaacaaagga
    31201 gttcaatgca atcagctgag atagaaaaat ataaacaatc atcggctaag aagaaggatt
    31261 ctaaaaaaat agagaaagat atggatgaac tggaaaataa agtggctgtt atagcggatg
    31321 actttttttt aggcgattgt gcaaatgctg cagtggataa tgttttgcaa atgtataaag
    31381 aaaagaaaca ggaaatattc gatagtttta ataaagtaaa ggaacaggca gatagagttg
    31441 ttcagcaaca aaaagctaat tatgaggcaa taatggcaat taaagaagct tatagaaaaa
    31501 actatgaaga aatcaaggga aagcataagg agagcattga aaaaattaaa gaagaaataa
    31561 ataaagtaca tgaccagtaa gttagcgtta cgataataaa aagtttttac cggattggac
    31621 gccctgttca aatccttcca gaattttttt tgggtcacga gttaatctat ctacagtaaa
    31681 atcatccgga ggatagataa tatctatgtt tttgtgttta tgttccagtt catcaagaat
    31741 agaattgtac atgaggtacc tgttttccag tgcctgaata agatagcgat attttgaatc
    31801 cttaaagtaa ttttcaataa gatatctgat ccagaatgat tcttcttttt ttctataccc
    31861 tttttttctg gttacaacaa caagtatatc ttcttccttg taaccaaggt ttaaagcttt
    31921 tttatacggt atgggatcaa gcaatcctcc atcacatagt ttgcggttgt tatattctac
    31981 aaatccacga tataatacgg gtattgcagc agatgccttt aatgatgtta taatatcatc
    32041 tttccgtgaa tgaagatata ctgtagatgg aggattatta tcgctgtact cagtggcggt
    32101 tatcaatact gggatagagt gtttcagata ttgaatattc aatgtttctt taccttcaat
    32161 gacagttgaa accatccaat ctaaatttaa tatatgcttt gttggccgga gtatatttga
    32221 tatttttata aaactttcac ttaacaggac tccaaaaaaa gcatcgcgtg ctaaataaat
    32281 ttggtgtgcg gagtatgcaa cacctgttaa agcacctgct gatgagccaa taattaatgt
    32341 aaaaggaaaa taatctctgt cagtgaaagc ctgtaaaact cctgttaaaa aaactccacg
    32401 catacctcca ccttccagta taagcacctt ctttttaggg aatgatttct tttgactttg
    32461 tttttttata tatctaataa tcggagaaac tatgagcata taggttaact cctgctacac
    32521 aaggatttag aatcatatat cattttgtga acgtctattg gagtttgaaa attttattct
    32581 tagcttgatt caaaatgtta cttattaccg tatttttaaa agatttagga gttacagcat
    32641 caacagacaa cttattgtaa aaaatacatt atttataata catgtaatgt atcaaaagtc
    32701 aataggaaaa ataatagtat tgacagtgtg ttttttacat tatacattta aacagtctat
    32761 gctaatatgt taatccttaa tcattgatta gtaatgaatt gtaaacctct tatgcttgga
    32821 gggtagcatg gaagcaattg tagtacagca tggtgaagct gtagatgcat cggtgaaccc
    32881 tgaaagacca ttatcaaaaa agggtcagga gcaatttgtc agattagctc aatttctaaa
    32941 aatcatcaac tggaaacctg aaattattat tcatagtggc atattaaggg cctttgaaag
    33001 tgcggcgata gttgctgaaa catttaattg cgaagtcgca gtaaacaaat ctataaaccc
    33061 taaggataat cccgatgtca ttatacagta tttattgctc gaaaaaagaa atattattgt
    33121 agtaacgcat aaaccattta ttgatatact ggttgcaaaa gtgttatcaa ctaatcaaac
    33181 cggtctaata actgtaacaa atgcctcccc cattttttta tcaagtgatg gggatatgct
    33241 atattgcaag gcatatattc ctgaaatgat gatagatgcc attattcgtt ctttttcaaa
    33301 ccggcaatca taagaatgat aatacctatt atggaccata caaaggtacg caatctccgt
    33361 acaataccaa tagttagccc taatgaaggg tcataaccta acagtttaag agccaatgaa
    33421 tacgagcctt cactggtacc caaatttgcg ggcatgaaaa acatgacaga tgttaaaaaa
    33481 agcccaaaca catacagaaa cagtgcatgg tagatattaa aagttatatt aaggaaatat
    33541 acaattagat atatctctaa tgtgcaggaa attgtcactg cgaaataatg aagcagtatg
    33601 gatatgatga acttatgtat gttcgatttg ctgctgaaaa tatatttaat ttcttcatcc
    33661 agctcttcaa tttttttccg ccttccatct gtgattattt tactactgag tcgagaaggt
    33721 atcattgaga taatgaaatc cagtaaacct tcgcgctgtt tttgaataat taaagcaata
    33781 atcaccagtg ttagaagaaa aaatcctaca gcaattactg taatgtatac tggcaaattg
    33841 agtatgaata agcttaatgt aattcctgtt aaaaacatta atgcatttgc cagtgaatgt
    33901 gctgttctat ccagcactac cgaggccaat ccaatctgta acgggatgtg gtctttcata
    33961 tacattgctt ttaatggttc cccggctatt gagccaaaag cattgataga agatgttgaa
    34021 tcgccaccaa tacgggcaag tataagcaat ataaaggttt tcaaggtaac aggatggtta
    34081 attaaaactt tccagcccag ggtaagaaaa atatggctga agataaatat gctgacgatg
    34141 atccagaatc cccatccaac agataagatt tgtgctattg ttttttgaag accaaaagtt
    34201 ttaaaaagaa aaacaagtat tagtattcca ataaataaaa aaatgtaacg agcttttttc
    34261 atgtggtatc cagaaaaatt agaattgaat ggctagaaca gtaaaatcat ccacaaattc
    34321 tttgtcaaca tcctgagtaa caagattatc ctggagtctt tgaataagaa aatcgggtcc
    34381 tttttttatg gattgaagcg cgatttcaat gagttttttt tcgcctgcat ccatgttccc
    34441 gatgcccaat agatcaagaa ccccatctgt aaaaaggaca acaaggtcgc catcctcaaa
    34501 aaacaaggcc ttttcttcat attttgcatt gttggttata ccaagtgcta ctcctttagc
    34561 attcagacga attgtctcca gtgtttttga acgtattaac aattgattat tatgtcctgc
    34621 gctgccatag gttaagagtt tattatgtga atcaatgagc atgtaaaaaa ctgttacaaa
    34681 cataccatat tcagaatctt cataaatata tttattggca tgggtaagca gttggccggg
    34741 ctggttgttt atacgggatt ctgctcgcaa tacgttccgt gctgtaccca taaatagtgc
    34801 tgcaggtatg cctttgccag atatatcagc aataagtaat ccaaattttt cattatcaaa
    34861 ctgaaaaagg tcgtaaaaat cccctccaac ctcttttgct gatttattaa acgctgctaa
    34921 gtggtgattg ttgattgatt ggggaatttt tggaagtatt cttttctgga tttctgcagc
    34981 aatatcaatt tcctgagcaa gccttcgctg ctcttcagtt tgttgtaaat acagtgaatt
    35041 ctgataggct tctacaatct gattggctat ggtggttgca actctcaggt cataggcatc
    35101 aaaactggtt ttgtctaatt tgtctgctaa gtttaaaacg ccaataacag tatttttgta
    35161 tcgtaagggt atggagataa atgagggggt aatatattca tgaatttttg caaaagaagc
    35221 aagcgatggg tctgctataa tattattgac cagtaaaggg tccccacttg taaaaacatg
    35281 ataggcaata ggtgcctgat ttttacttaa tggggaattg atagattcag gaagattata
    35341 tgaagatgca atcgaaaatt tgtctgttga ggtgttatat aacagtatgg atgctcgttt
    35401 aacgcccact gatgaggcaa ggggctgaat tatctttgaa aatatattct ctgaaggggc
    35461 agaatgagca atggcctggg atatctcata cattatttca agttccttaa ggcgggttgt
    35521 taaatcctga tagagctggc ggtgtgtaat tgcaatagct gctgaatcag caatatacat
    35581 caaaacttta aggtcatcct gagtaaatga atccctccca attgtgttta ctgcctcaat
    35641 aacaccaact aacctgtcaa caagcaccat cggagcgcag ataatatttc gagtatgaaa
    35701 atgagccttg ttatcaattt ctttataaaa cctttcatca ttttgagggt cattaacaat
    35761 cactggggtg ttggtaaggg caacatgacc ggcaatacct tttcccttag ggactatctc
    35821 ccctttgata agagagtcac ttccccctct gacaatataa aaatataggt cttcggtaac
    35881 aggatcagtt agaaggattg aacaaccttc tgtttgcaac atatctttgg ttatatcaag
    35941 aatgaccatc aacaaatctg aaagtttcag attggaattg attcttttat taatagcgct
    36001 aaatttttca atatcatact gtaacacgaa ctttcaccat gtaccttaaa aaatatctgt
    36061 ttaactggaa ttacccgtga tcctgcatcc tgaatgtata tgattgaatc aaatctaaaa
    36121 taatatttac tattacaaaa taattttata aaatgtctat ctttttttaa gcaatcgata
    36181 ctgaattgtt atatacgtcg atgaatgtca ttgtacattg taatggtatg ttcgatttag
    36241 tattctataa gcatttttct atatttgtat agtgatggtt tatatgaaag ttattgttga
    36301 attatcgctt acaccagatt tcaaggaaga ggaattatca aaggaactat cgctcctttg
    36361 tggatgtacc attacttcat attctattct gcgtaagtcg ctggatgccc gcaagacacc
    36421 tgtataccgt tatcgatgct gcgtggatat accacaccat gttgcaacaa cacttatatc
    36481 cataggcaaa gcaactgcct ataaagaaga ggaacctgta ataattccaa aagtaaaaac
    36541 caataagcgg gtaccaatag ttggatgtgg ccctgcaggg ttgttctgtg ctctgacact
    36601 gattgaggct ggtgtagccg tggatatcta tgaaaggggt aaaacattag cacagcgaca
    36661 cagggatgtt gctatgcttc gtgaaaaggg gatccttgat gccaatagca atgttgtgtt
    36721 tggcgaaggt ggtgctggca cgtattcaga tggcaaattg actacacgca ttgataaaaa
    36781 agaaataaag cttttttatg attttctggt gaactttggg gcaccatcgt caattgtata
    36841 tgaagccaaa ccacatattg gtactgacag gcttattggt atcattgaaa atatccgaaa
    36901 gtatttaatt cagaaagact gcacaattca gtttggtaaa aagctcaccg atttacgtat
    36961 acataacgat acactgcagg gctgttactt taataataca gaggagatag ttactgaaat
    37021 actggtcctt gccacaggac attctgcgcg ggatgtatat caggtgttgc atgccagagg
    37081 tgttgcatta cagaaaaaag gctttgcggt gggtgtacgg gttgaacatc cacgagaact
    37141 gatagatgaa attcagtatg gtaagggaaa agatgggtat ggtctgcatg gtgctgagta
    37201 tcaattaacc tttaaagatg tcacagggag aggagtgtat tcattttgta tgtgcccggg
    37261 tggatttgtt attaattctt cttcccagga agaacgattg tgtgtgaacg ggatgagtta
    37321 ctcaaagcgt gatggaacca gttcaaatgc tgctctggtg gttaccgttt cacaggatga
    37381 ttttaaagat atttttgatg gcattaattt tcagattttg ttagaagaaa aagcgtatca
    37441 ggcaggtgga ggtggatttg gtgctcctgc ccagcgtatt acatcttttt tgcaaaattc
    37501 gattgattct tcattgccgg tatgtacgta taaaccaaag attgctgcaa gtaattttaa
    37561 tacaattctt cctgatttca tcagggatcc attacacagg gctatcgtgc actttggatc
    37621 aaaaatgaaa ggttttatta caaacgaagg gattttagtg ggtgtagaaa ccaggacatc
    37681 ttctcccata cgaatagttc gtgatgaaaa ttttcagtca attaattgta aagggttatt
    37741 ccctgttggc gaaggtgccg ggtatgcagg cgggattgtt agttcggcag ttgatggtat
    37801 tcgtgcagca caaaagatag taacattatt agagagggta taaaacaatg attccggttt
    37861 tattgcgatt aaaaaatcca atcaatattg atgaccgaca ccattttcca cggccagatt
    37921 tattacgatt gccaacctac aacctcaatg gccaatgggt gatagtgcct gatagaaaaa
    37981 atcgtggaac aatgttgcaa tggtatacta agaccaatgt tcagaagata ctgcatcaat
    38041 ggtataacat tgatgaattt gaatcatatc ctgttatagt cccattcccg ctggaagctg
    38101 ataaaaataa cgagtttcat gcacaaagcg gaataacacc agaaaaggtg atatcgcata
    38161 agcgttactg gtatttctgc tcattttcca ttgaagatat tactactgga atacttcatt
    38221 ttggtgcctg tgattacaaa gctacagtgt ggctcaatgg taagctttta ggcacacatg
    38281 agggcggata cactcctttt tcttttttta ctcattttga agatacaaat tatttagcgg
    38341 tactggttga ggacagcctt tcaccatcgc aggtgcgggg taaacagaca ttccttaata
    38401 aaccctttat ggtatggtat accaatataa ccggcatatg gcaggatgtc tgggttgaaa
    38461 aggttgggtc atcgtatata cgttcagtta cctgccagag aacgggcagt agcgtccgct
    38521 ttattattga tgtggtaacc agcgaaaata ttgcaaatga tttcagggtt gaattgttca
    38581 tctatgcatc acaggtacat ggtaaaaaaa gtgcgttaaa aacacctgtc aaaacatatc
    38641 gtgatattgt gatgtttgat gtttttaaaa aaggacaggt aatatttgag gtacctgaat
    38701 cgctttttgc tacatggagc ccacagtatc ctgcacttca tcctcttcag atagtcctga
    38761 aaaaagggac aaaagaagct gactgtgtgt atagttattt tggcagaagg gatattgaaa
    38821 tcaaaaatgg ctctattctt ttaaataaaa aaaatctcta tcagcggttg ttgctcaatc
    38881 aggggtatta tcctgaaggg ctgtatagac ctggtgatag ctccatgtac agaactgata
    38941 tagaactcat gaaaagtatg ggttttaatg gatgccgtat tcatcagaag atagaggatc
    39001 caaaattttt atactgggca gatcttttag gatttttagt gtgggaagaa atgcccagtt
    39061 actatctccc attacccaaa aatatgaaaa agctctatac acagcttcag gaggtcatga
    39121 aacgggattg tatgcacccg tcaatcatta cgcttgtgtt atacaatgaa agctgggggc
    39181 tttattccat gtttttttca aaaagggtgc gcgatagtct catggcattg tataccaggg
    39241 taaaatctga ttttccggga tatatggtca tagataacag cggatttcat catgtaaaaa
    39301 gcgatattgc cgatatacac cattatctga agcgatttga agatattgag gaattttatc
    39361 gaaggcttgt tgcaggtgtt cgcgaggcac cactgtgggc aaattttata tccatgattt
    39421 tgggcaaaga aaatattcaa acaccgtatt taaaaggata tgctgattcg caggcaccgc
    39481 ttttaataag tgaaatgggt ggatttggtt ttggcatgta tcaatcggag gctaaaaccc
    39541 tggaagaaag ttttcagaaa cactgtgcca tccttgcaaa atttcctcac attcagggtt
    39601 tttgttatac ccagtttacc gatacattcc aggaaaaaaa tggcgtatgc acgtttgacc
    39661 gtacaatgaa agcaaaactg ttgcccatca tgcaaaaagc gctgtcaaaa catatatttt
    39721 ggtaacaaat atatacatta cagtcgataa aatgagtaag gtataatatt cattgtacga
    39781 tagataatga tatgaaaaaa tttgcattga tagttgtttt catcctgctc gcaagtgcta
    39841 tttatcccga aggtggagac taccttaggg taacactggt aagcccaaaa ttatcctatg
    39901 atgaaaatga cgatgtcatt gtatactgta ttgtgcataa cactgctggt gtaccggttg
    39961 aatttaccgt gtatgatgcc ccctatacca catttcaacc tgtggtatat gcacttgacg
    40021 gcaaagaagc ttctctaaaa gttgactatc gcttgaaaaa catcccaaca gcacaactgc
    40081 ttgccaatga gcaggggcgg aaaataatat tacagccgga tgaaaagttt gtgtatacag
    40141 catattgcaa taccttctat gccctaacaa aaaatgaaaa ataccgggta aaagcgtatt
    40201 tttatccaga tgcaaaagat gactatgtta taaaatctgc taatgaagta actattacaa
    40261 ttattccagt accacagttt gccatgcgta cggggattat caaacatgaa cgtaaagtga
    40321 ccccttcaga agtggttatg ctggcattaa tggccgaaaa gaacaaacag tgggaaaatg
    40381 ttgttaaata tataaatatt gaaaaattta tctatgccta ttcagaattt gccaggcgat
    40441 attcaacaac aaatgattac gagcgcttga ttgtgcagca ggattttatt aattttatta
    40501 caaaagagcg tcctgattat ttgattgact tttctatact tggcgagcag ataatacccc
    40561 agactaatgt ggcatatgtg gatgttaaag ttaaacgatg ggggccccgg ttccctgcaa
    40621 taatgaaata caggtatact ctggaaccgt ataaggattt ttgggtgata gttaatttag
    40681 atgcttcaat agttaaggaa taatctatga cagagatact ttcacaggat gaaatagatg
    40741 ccctgttaac tgccatctca acaggagagg cggatacaac agattacact gcggtcaaag
    40801 agcagcgtaa ggtcaaaatc tatgatttca gacgacctga taagttttcc aaagaccaga
    40861 tacgaactct gcagatgatg catgaaacat ttgcacgttt gaccactact gctctgtcgg
    40921 cacaattacg agcgcttgtc agtgtacacg ttgcttccgt tgaccagctt acctatgaag
    40981 aattcatccg ttccataccc aatccaacta ctctggctgt cattaatatg gaccctctga
    41041 aaggttcggc ggtactggag atagaccctt ccattacttt tactataatc gataaacttt
    41101 ttggtggtac aggcgaatcc accaagataa gccgtgaact gactgatatt gagctatcgg
    41161 ttatggaagg cattatagtg cgaatacttg gaaatcttcg tgaagcatgg tcaaatgtaa
    41221 tagatttgcg tccacgcctc ggcaatattg aaactaaccc tcaatttgct cagattgtcc
    41281 cacccaatga catggtcgtt cttgtgacac tggaaacaaa ggtgggcgaa gttgaaggga
    41341 tgatgaacct gtgtatacca tacattacca ttgaaccggt tatttcaaag ttatctgcgc
    41401 agtactggta ttcaagtatt cgtaaaggtg ccacagacga aaacgctgca attattcaaa
    41461 gccgtcttga agcagttgag ctacccatag ttgttgaagt aggtgaagta gaaataacca
    41521 tggaagaatt gcttgcaatt caggcaggtg atgttattaa gctgcccaat actaagatta
    41581 attcagacat gatattgaaa gtaggcggaa gaaagaaata taaatgtcga ccgggggtta
    41641 ttggtaacag ggttgccgtg cagataggcg aaaagataga agaaatccct gatgaactgc
    41701 ttgtcagcaa gcgtagtgaa gaagaataat atttaataaa ataacaatta ttgatttaca
    41761 actttattta agtagggaag cggatctaca aattcggtcc caattttaat ctgataaaaa
    41821 catatatggc gtgtggcgtt gcctgttttt ccaacatagc caataacttc acctttagtg
    41881 accttttgcc cttcttcaac agtaaccctc tggcaatggg aatacactgt aacaaaacca
    41941 tatttatgtt ttatagatac agataatcct aactgactat cccatcgtac ggattcaaca
    42001 acaccaggtg cggtaactct gataggagca cctgggaatg atgctatatc aacaccttca
    42061 tgaaattctt tctggaatgt atagggagat gtccgtatcc catatcgtgc aataacaaag
    42121 ccatccacgg gccacataga tggggtgttt tcaatcactt tacgccgttc acgcatgaat
    42181 tttttaatat ctttaagaac atcttttgca gtttctaaat cctgtaccat ttcctcaata
    42241 tttaaaattt caataggcgg ggagggctgt tcctgttcaa catcaatatt ttcagacaca
    42301 ccaccttttg cccacagagt atcagccctg tttccaggtg ttaatgaata aaggtacgat
    42361 agttccggtt ttaaccgctg aaatatatca tataatttat taatttcttc tttatacttt
    42421 gcaatctgaa ttttagagtt tgaaccatac atcttcaggc gggatacttc ttttattgtg
    42481 gatgtatggt tgattataaa aatagatgtt aaggttaaga taagtattaa ggagacaata
    42541 atacccgtca gtgcaaaaag agtaacatgt aaattaacaa tctttttctc tgaatgagga
    42601 ataaccataa tggttaatcg ctcatttcct ctttttttta tatattccca ctgagttagc
    42661 agtttattgc gaatatgacc ccatctctct gatatgagat cctgtagttg tttctggagg
    42721 tcgcttatat ttgattcttt aaatggattc aatcccatag atttgtttaa accttttttt
    42781 attatgatat atatcaatat gaattatgta ccatagtaca tatacaagat atgtataaaa
    42841 aatagcctgt ctgttgtaat agtcggccaa ttattgtcaa gtttattctt ttttttgctc
    42901 gtacactgat tagcctgttt attgaataaa gaaattacat tattaacaat atgaaatacg
    42961 ttttattata ataatacata ttaggtttgg atactataaa tatgaaactg aaagaaatat
    43021 agcagataaa tattcacagt aaatttttta aaaaaaattc taaagcaaaa atttaagatt
    43081 ccgaaatgat gattaaggaa aaatatgtat aaattttgct aaatagataa aaatggatga
    43141 aaaattgcta tatttcacta taaatgctta taaatggtta taaatagata ttttagactt
    43201 taataatata catattgcta ccgaacatca caataagctg taaggttctt tgaaaactaa
    43261 aaattagtag ctgcaacgcc atgtacttca ttttttaagg tatagcatat accagttaaa
    43321 atgagggaca gggggttgta gtgaaataaa aaagggtatt ccctttttta agaatcacca
    43381 acccggtttt acccctgata gggagatcag ggacaattta acacaaagga gtgtgtgtat
    43441 gattattaac cacaatctaa cggccattaa ctctaacaag gtcctcaagt ttaaacactg
    43501 ggacgttgac aagtcaatgg aaaagctatc atccggtatg cgtatcaacc gcgcaggaga
    43561 tgatgcttca ggtctggctg tttcagaaaa aatgaggact cagattcagg gtttgcgtca
    43621 ggctgaacga aatactgaag atggcatgtc gtttatacag actgctgaag ggtatttaaa
    43681 tcagactgca tcaatactcc agcgaatcag ggttctggct gtacagtctt ccaacggaat
    43741 ttatacactt gaagacagac agctcatcca ggttgaagta tcagctctgg ttgatgagat
    43801 tgaccggatt gcatcacagg ctgaattcaa caagttcaaa ctattacttg gtgatttctc
    43861 acgaaccaat ccaaaagcaa gtatgtggtt ccatatgggt ccaaatatgc atcagcgaga
    43921 aagagtatat attggaacca tgacttcatt gggattgaat ttaagagaaa aaaccgggaa
    43981 gtttctggca aatctttcaa ctgcagaggg tgccaacaga actattggta ttgtagacga
    44041 agctttgcag aaaatatcca agcagcgtgc agacttaggt gcttactata acaggttaga
    44101 atatgctgct aaaggattga tgaatgcgta tgaaaacatt caggcatctg aaagccgtat
    44161 acgcgacgct gatatggctg aaacaatggt agatttaacc aaggatcaga tccttgtaca
    44221 gagtggtact gcgatgctgg ctcaggcaaa tttacggcca cgaacaatct tacagctttt
    44281 aggaacttaa tataaaaacg ctgacatgta gcagggaata cccgcccaaa caatataccc
    44341 gccccctgtg gcgggtattt ttatgcgtgc gatagttctt ctaatattct taaaatctcg
    44401 ggtagcaact gttttttccg ggataaaatg ccaggaaggt tgtacatatg tggcccaaga
    44461 ggtgaaaaac ttaatttttg ttccccagta aaattggtgg aaaagagtac actattctga
    44521 gtggtgatat cagttaccag tagaagtgcc cagtgaacat ttctgctttg tgcaaattgg
    44581 gaaagagcgg aaagtaaaga ttctttgaca ctttcaatat catcaagcgt aataacctct
    44641 acctgagata tggctactga tataccgtat tcctgatata tcttacagtc attgctgact
    44701 gcatacatgg ggtctatagt cttaaggctt tcggtagatg aaagaagttt cttcccgtac
    44761 tcttcatagt gtataccagc tattgctgac agctcttcca gtgctttttt gtcaatgtca
    44821 gtgcttgtag gtgataaaag cattgcggta tcagataata ttccgctaca aagtagcatt
    44881 gctatgtgag gctcaggctg tatggcatta tatttgtaaa gttcatacac cagcgtacag
    44941 gtactgccta ccggtttaga atatacaaat attggagtgg tggtttttat tgcacccagg
    45001 cgatgatgat cgatgatttc aatgatatgt gcagtttcgg caccttcaac tgcctgcgat
    45061 aattcattat ggtcaaccag tatcattttt tttgcataat gcttaatgag atcagaacgg
    45121 gtgatgatac cggttagttt accattgtcg cataccggaa gtccccgata ttcgctgttt
    45181 actatttttt cccgtgcctg catcacagtg ctttggggca aaatagtatc tgattgtttc
    45241 ataatgtaat ggcagggaat gcttaaggta agcaggcgca atgtattgaa tgtatcaagt
    45301 tgtgaaaggt atacccagcc tgaataggtg gcggtatcgt aattaaaatc ctgtgtgccg
    45361 gttacaatga tcgcagcaaa attattgtga attgcaaatt caattattga ttttcggttc
    45421 ccaacaatta gtacagtgtg ttctggttta acatttttta acacattgag agattgggcg
    45481 gattccattg cacctatgac aatatggcct tcaaattctt cgttctgccc ctttttaatg
    45541 caacgcccgg gaatggttgt agtaaaatta gcaggtttaa agtaatagag tggttgtgca
    45601 atggatgtgg cagaaaagta aaattctgcc agttctgcaa agctgaccac ccctttatag
    45661 taactatcgc cgcttacaac aggtattata cgcacacggt ctttttcaat gtgttttact
    45721 atggaaagca aggggtcatc aggatgggca gttatgacat tgcgggtcat tatatcgctt
    45781 actttaggat atacatcttt cagcatttcc ggtggctgaa ggttaacctc ttcaaaaaca
    45841 aagcgtgttt gcctgtttac attgccacag cggtaagcgg tgtaatggtt atgagtgtca
    45901 ataatgtttt tcaggtgtgc ataacaatat gctgcacata ttgaatcagt atcaggattt
    45961 ttatggccaa tgacaataat ctcttccata aaataatcag tgctctcttt ccaggattgt
    46021 tatagctttg ggatacacta atgctgttat tgcataaatt tcccatgcaa atgttgcaaa
    46081 cccaatatac cccagaatgg gcatttcaaa taatttaatg tgcgggagga ttggtacggt
    46141 gtatatccat tttgttgctg cccaccagtt ccagaattcc cataagtaac cgcataatag
    46201 tcctgcaagc ataagtgcta taagttttgt gtatttaccc agcacaagct cagcatagag
    46261 agaaggacgg ttaaggagat agttaagcgg ttccagaatg aaaataaatc ccagccatat
    46321 aaaacctgca gtaaattcac tgtaaaacgt atttgcagac catgaaagcc agatgaaaag
    46381 gtcagggtct gctgctctgt ttacgtaata cattgaaaag ggaattattg gtataaaaag
    46441 gaagaatagt ccaataataa aggacgcaat aaggaaagag gatgtcaggt gaaactgttt
    46501 atagagaatt ttcggtatga ttgtcaatat aatcccatac actaagagga ctcccgggaa
    46561 tattgttgca aaagcccata tataaccaag gtatctgata tgcagtgttt gcagtaaacc
    46621 ctgataatgc cagtttgaaa aataaatgtt aaaccactca aacatccacc aggatagtac
    46681 tgaaataact gctaatgttc ccagtgccct gttttgtatt aatgaacgat tggtttttgc
    46741 aagtatcccg tcggtgagca agattaaacc tgtccataag gttggtgttg cccatatggt
    46801 taccatacga atatccagca ataaacatat ttgcatgaat agtatcagta agattcccgt
    46861 ataaaaataa aaagggtacg atttcatacc aaactcctta aaacccaggt taacatgtta
    46921 gccatgccag aattcatcca ggattgattt aactgctgca gatttattta atggataaaa
    46981 atgaaaacca ggtacgccat acgattgcag ttctttacac tggttgatgg tgaattctat
    47041 gccaacttta cacatatcat cagggagagt gcatttttca agctcttgca gtaaagctac
    47101 gggcatgtca ggattgcaca tggtggttac cttttggagt tgtgccatat tggtaagcgg
    47161 cataataccg gggataatag gaatactgat acctgctttt gcagttttat ccataaaggt
    47221 atagaacctg tcattgttaa agaaaagctg ggtaatgatg aaatcagctc ctgcatcaac
    47281 ttttttcttt agattttgaa tatcagtatc taaatcaggt gcttcaatat ggccttcagg
    47341 atatccagca acacctatgg taaaatgatc catggtgcgg ataaattcca ccagttcgct
    47401 ggcatattta aagccttcag gatggggtat aaacttgtgt tgtcctgctg gtgggtcacc
    47461 acgcaatgcc aggatgttgt taaaacccag tgattgaaca tggtttagat agcttgcaat
    47521 atcatccttg ctttgcccaa cgcaggtaaa atgaattaag ggttcaattg aaaatgtaga
    47581 ttttatacgg gttgctatct ctaatgtttt ttctctagta gaaccgccag caccataggt
    47641 aacggagata aaatcaggtt taaagcttac catgacatgt agctcatgaa ataaattttc
    47701 ttccccaatg ggagtttttg ggggaaaaac ctcaaacgat aacagtggct ttttttgctg
    47761 taaacgggta atgatattca ttgtgctctc tcacgtcttc ataatggatt gcatgtaagt
    47821 gttacacaca cagaataaaa gaagtagctt ctgttttgta ccactggagt atattgaaac
    47881 atgcagaaat attcaattag tgtgacaaaa aaatcaataa taattgtata taaatcaacc
    47941 ataaataaag aaataaatat taatttcttg acacgcataa agtaatgttg catactatcg
    48001 aagacagtta tgtttgaaaa taatgtatta tttcaatcat atctgttacc gctaatattt
    48061 attatattag ctatgatatc aaattattat taaatttctt tacttttttt accgatgatg
    48121 tttatgatat catagtaata tgatagtggt gaagtagtga aattgtatat attgttccat
    48181 aatctttttt ataaaggagt cgacaatgag aagggaggtc aaagctttta tattgtcgct
    48241 tgccatcggt agtctggtat taattgtcgg tggaggcaat ataatggtgc aggcgcagga
    48301 agcgcagcaa ccaaagaaac agcaaacagg tttagttgaa gactggatgg atgattttga
    48361 agcatgtgag gactggcgtg cagcttcaac atgccccata ggtgaaacaa aaatcaggaa
    48421 aatacctgga aaaccacgtc ctatagatga taatggaaat gagatcaact atcctgatac
    48481 aataactgac gacaatggta taactcatga gaataaattt gttctgggtg ttaaaactta
    48541 ttatttagat aggggatatg acagagttga ggtgttcccg ccaaatgaat atatcatccg
    48601 aggaaaagct aaagaagtta aagtgtgggc tttaggtaga aaattcaggc ataccctttt
    48661 tatcaaactt cgggattata aagggacaat tcataaaatt aaaataggac ggcttgattt
    48721 ctggggatgg agggaattat ctgttgtaat cccgggctgg ttgcctcaga gtccaggata
    48781 tgcattactg gacaagaacc tgcatttcgt ttcctttttt gttgaaagtg atctttatga
    48841 ggttcctggg acattttatt tctatcttga taatttcaga gtcattaccg atctgtctga
    48901 attcactggt gatacaagca tcagagataa ctggtaaaaa agcatgatga ccaaatatga
    48961 gacctcggag gcattaatat gaaatataca cgatttattg cactatgtgt tgtgatagca
    49021 agtgtggtgt ttgtaggggt tttccctgaa aaagcacaat caagaattgt tactaatgtt
    49081 cagcctgaca taagtgctga gcaatacaat gcacttgttg tggaagattt tgaaacacag
    49141 tcagattggg tattggagac agttcctaaa aaacatcagg atcccaaaaa agatcctgta
    49201 ccagtactgg aaatgaaata tgttgatgga ggaccagctg acctgattcc ggaaaagtgg
    49261 agccctctca acaagggatt ggaaaagaag aaatgtttag gcattcactt taagtttaaa
    49321 tatcccggat ttaattctgt tcatatatta ccgcctttgg aagttcgctg ggatgatcca
    49381 actcagaaag tattaacata tgactcccgt gctggtcagg aagttcagga acgtgctata
    49441 cagcttccag gaagagcaaa agcggtatct gtatgggtac atggcagagg aaataattat
    49501 gacctggaag tttggataaa ggattataaa ggcgatgtgc atatattaaa gatgggttcg
    49561 ctgaattttg taggctggag gccaatgaag gcatatatcc ctgagaatgt gcctcaggaa
    49621 accaattcct atcctcagac acgggttacc aaaattgtac gttttgtagt cagggcaaaa
    49681 cctgaagccg gcaatgagga tgtatatctg ttttttgatc agatcaaagt attaactgat
    49741 acatttgagg tcaattttga cgggcagaat ctccatcaat taatggaaaa gggtggagga
    49801 acaaaataga ttttattatt aagttgaaaa atcatttaaa caaacaaacg ccacggtatt
    49861 ccgtggtgtt tgctttatct ttatatggta aagaaaaaca tacccttata taaaagttct
    49921 tgctttttta gtatagaaaa tttagagtat acctagaaat ctggtatatt attaattcct
    49981 tcctttaaaa taattgcggg atcaattttt gggtgaatgt actttgaagg gtgaggtagt
    50041 atttaataga aattaaagcg atggccaaaa ctatacaatt atttttatat gtagtatgta
    50101 aatatataat actgtgttca ttaatgaggg gcagatatgg aaaaggaaaa gttgattgaa
    50161 cgtgtaaatt caatgtttaa agaagagatg tggggaagac ttgacccaaa agatgttggg
    50221 atatcacgtt ttagaatact tgatgatctc ttcaattcaa tagttgctgc aggtattgta
    50281 aaagaagtta gggatatatg taaggaacac atccagagtc atcctgattc tattgttgca
    50341 tcgtatctta ttgggttaac cggatatcat cttgatatta ttgatgataa aatggagatg
    50401 cgacggctca ttgatatgta tcaaaaatat cataaatggg ttattgttga gcttttatca
    50461 gaaaaagtcc tggaatatgg ggaaagcagt tttgcattaa aatcattggc aacaagcttg
    50521 gagcgtttgg gaagaagcaa agaagctatt ccagtttggg aaaacctgct taaaatagat
    50581 agatttgatg ctgaagtggc aaaaaaatta gcagttgccc tgatcgatga ggataaacca
    50641 aaaagtattc agtacatgaa gctttctatt gaagggcata ttaagaatgg gctttatgat
    50701 gaaattgctg acctgtggaa taagcttgta caggtatcat gggaagatat tcaatttttt
    50761 gaaagaattg aaaggatgct tattgaagca cgccaatatg aactgacagc atcactgtta
    50821 aaagtattgc ttcataaata taaagatgaa gaacatcccg accagtcaat tcagatacta
    50881 aagaagatac tggaatatac acccaatgat acagcagctc gcagagaact ggtaaaatta
    50941 tatgaaaaga aatatggttc tcattcacag tttcagctat tcctgaaatt atcaaaactt
    51001 aacaatttta aatatcctgt gaagcatgct attcaggatt ttgaaaacag tataattttt
    51061 gataaaggcc attatgtacg acatagaagc tggggtgtgg gaagaattgt cgatattact
    51121 ccaaataaca taataattga tttcaaagaa aaacccggac atcagatgtc tgttcaaatg
    51181 gcacttcagg cattaacgcc actgaaaagt gatcatatcc aggtattaaa gtatgaggac
    51241 tatgaaggcc tgaaaaagat ttttgaagaa gatttcttac agttttttga aattcttatc
    51301 cgttcgtaca atggcagtat tgcattagca gatgttaaaa aagaactgat accggatttt
    51361 gtaagtgaaa aggcatgggc aaaatggtgg agcaataaaa gaactgaaat taaaaagtcg
    51421 cctttgtttg gtatttcacc agagaaaaaa gatgttataa taatgcgtga taagcccctg
    51481 acgtttgctg atgaattgct tgataatttc cttaagacgg attcatttac cgaaaaactt
    51541 gatgttgtca ttgagtttgt aaataatatt gatgcaggtg agggagttgc agtagctcaa
    51601 ttctttgttg actatttttc tggggaactg aaaggagatt cggttacaag gaaaatctta
    51661 tcgtatttta ttttaaacgg actgagcaag tttattgatg ctaaaaagct taaacttgat
    51721 caagcatata aaggtatagt tgcaattgtg aaagagtcac aggatcttcc atcaatatca
    51781 cgtaagatag ggtcgtatga ttataaaaag gatttgatca atctcattaa agaagccaga
    51841 gaagattggg ctgatatcta ttcgcaaatc ctttttgaaa caccagtccg aatacataag
    51901 tatatcgtta atattttaat cagatcacat gaatatggaa ttattaacag gtttgttgaa
    51961 aaaacactta ttggggccaa agaattccct gaaatatttt tgtgggtagc cagaaacctc
    52021 cttacaaaat catgggatta tgaatggctg gattattctc gtgaacaact agtgcttact
    52081 ttattcagat taatgaatga gctaaaaaaa attgagcaaa aaggaagtaa acttaaaaac
    52141 atagctctgg atattatatt tgataatgat gcagctgtat taaaagatat tgttaatacg
    52201 tttgacatcc aatttttagg aaaagtatat gatattgttg gcagtattcc atatattgaa
    52261 gattcaatgc gcgataaatt ctttgaaata attaaatcac gctatccaga tttcaaaccg
    52321 ttgatgaaga ccgctgaaga agaatggaag gttgatgctg aagtgcttat tacaacgcag
    52381 gaaggatatg tgcgaaagca ggaagaactt aacagattgg taaatgttga gctagctgca
    52441 atttcaaaag agcttgcaaa ggtttctgat gcaacaggag atgtccgtga aaatgtggaa
    52501 tataatgcct tgcttgaaaa acagtcaata ctcaaaagag atattaataa gctggagagt
    52561 gagattaaaa aggctcaaat acttgattta aatactgtct caacggataa agttaatatt
    52621 ggtactgtgg tcacaataag tggggatggt gaaaaagaaa cctttgccat tctgggacca
    52681 tgggatgctg attttgaaaa acgtatatta tcatatcgat cacctattgc gcaggcgtta
    52741 cttggcaaaa aacaaggtga tgtagttgaa atacgaaaaa atgaacagat caaacaatat
    52801 actattgaat caattgagaa gtataagcca tgaactcatt cagagaatat tttacctatc
    52861 gcaatggcaa attatactgc gaagatgttt cattggaaga gatagcggaa gagtacggca
    52921 cgcctgtgta tgtctattct tcaaaatgct ttatggataa atttacacag cttgataaag
    52981 cattgtccag ccatccgcat cttatttgct attcagtaaa atctaattca aatattaaca
    53041 ttttgcaaat gatgcaaagc gcaggttgtg gaatggatgt agtatcaggt ggagaaatat
    53101 acagagccat tgctgcagga gtccaaccgg aaaaaattgt ttatgctggt gttgggaaaa
    53161 caactgctga gattgaactt gctcttacaa caggaatacg tatgtttaat tgcgaatcat
    53221 tacctgaggt tgaaagaatt aatgcgatag ctcaaaggat gaaaaaaaca gcacatattg
    53281 caatacgagt aaatccagat gttgaagcca atactcacca ctatattaca acaggtaaga
    53341 aggaaaataa atttggcatc tcactgcagt tgctgccaca aattgtttct gctattaaaa
    53401 atatgccaca tatacaactg acaggactcc atgttcatat aggatcccag atcactgacg
    53461 ttgagccgtt tgaagaagct tgtaacaggg cacttgaagt aatgaataaa ctcaaggcca
    53521 tgggaatgga taaatgtacc acattaaatt taggtggtgg ttttggtatt cagtataatg
    53581 acgagaattc gcttgatata gccaggtggg caaaagtcat agacaatgca attaaagata
    53641 aaaatatatt tttgattatt gagcccggaa ggtatatttc cggaaacagt ggtgcactgc
    53701 tcaca
//
LOCUS       DAORYL010000009        53315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_521_length_53315_cov_11.111904, whole genome shotgun sequence.
ACCESSION   DAORYL010000009 DAORYL010000000
VERSION     DAORYL010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 53315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53315
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_521_length_53315_cov_11.111904"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1676
                     /locus_tag="PLV81_03345"
     CDS             <1..1676
                     /locus_tag="PLV81_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOM09169.1"
                     /translation="TDPLSEDNVVVFATGPFTGGHVWGGSRGHCASISPLTNILCDSN
                     FGGNFAAMFKRTAFDAIVIKGKASSPVYIHIDNEIVTIKDATSLWGKTVSEAHSLLSK
                     QNEKRIESALIGPAGENLVRYASVICSGTRVSAAGRGGIGAVLGAKLCKGIVVSGEKK
                     IEIFDVDKLKNCLNQHYPGMKERAKHLTEFGTPVLVQMINSRGKLCTRNNQKEQFDKA
                     YVISGEYIKEHHKKKNIACHGCPVACGKLVDVPYGTYTGTNVKMPEYETIYAFGSMLE
                     NSDVVSIYNANTVCDEMGMDTISFGVTLAFLTECVEKGLIKNSDLEIPLHFGKWNHVA
                     DIAEYAAYQKGLLGKLLAMGSRRLSEYIGGESYKYLYEVKGLEIAGHSARGLRQMSLG
                     YATATRGGSHHDTRPTYYPNDPEVDPGFTHQPEYSFNSQNNTAIGDSLVLCRFIHERS
                     FGTQINETLAEALSYVTGWDITKQELVEIGERIYTLERLINVSRGIDRQSDVLPYRVM
                     NEPIPDGPSKGWMCTNEDLQKMLDEYYRLRGWDKNGIPTQETLKRLKIY"
     gene            complement(1796..2800)
                     /gene="thiL"
                     /locus_tag="PLV81_03350"
     CDS             complement(1796..2800)
                     /gene="thiL"
                     /locus_tag="PLV81_03350"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOM09170.1"
                     /translation="MNLKDIGEFGFIKRITPDSVNNPVNVMRAIGDDAAVFAIGKNEC
                     VVLTTDLLVERVHFIKEAVTGYQLGYKSLAVNLSDIAAMGAVPQHAFVSIGIPKETSI
                     EYLDEIYRGMKDLAKQYNVNILGGDTTSSIVDLIINVVVTGVAKKKSLLYRNTAKEGD
                     VIFCTGLLGDSKAGLDFILKSEQPKNVQEKRLFEAHCLPRPHIEEGLFLANSGVVHSC
                     IDISDGLSSDLMHIAEESNVGFILQEDAIPVSPDLLHYCKEHGYSAIEYALAGGEDYV
                     LVCTADAKKATKLENDFYKTFKRTLYRIGTITKEKKYLMKRNDGTFSDIKPKGWDHFA
                     "
     gene            complement(2825..3466)
                     /gene="thiE"
                     /locus_tag="PLV81_03355"
     CDS             complement(2825..3466)
                     /gene="thiE"
                     /locus_tag="PLV81_03355"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOM09171.1"
                     /translation="MKTRFQSLLYLVTDRQLAKKPVEVVVEEAIKGGADCVQLREKNI
                     DTKEFVEIAQMIKYITDRYNVPLIINDRIDVALAVHAYGVHLGQDDMPLTIARKIVPH
                     AMVIGISVSTVNEAIEAEKDGADYIGAGSVFPTSTKDDISGIIGLEGIQEIKKAVSIP
                     VIAIGGIQIHNAEQVIQHGADGIAVVSAIVSSDNPYEAAKTLKTIICEVKSCY"
     gene            complement(3423..4250)
                     /locus_tag="PLV81_03360"
     CDS             complement(3423..4250)
                     /locus_tag="PLV81_03360"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HOM09172.1"
                     /translation="MELNEVSITSAIIDRYFQRLKNSLVSDVAIVGGGPSGLVAGYYL
                     SKQNIKTILFERKLSIGGGMWGGGMQFNEIVVQKDALPILEEFDVRYTQYAENYYTAD
                     AIESVCTIASKAIKAGLTIFNCISVEDVMMREDCVCGLVINWSAVDIAKLHVDPLTVQ
                     SRYVIDTTGHDTEVVKVVVKKVPGKLNTPSGTIEGEKSMWADKAEKFTLEHTREIFPG
                     LFVAGMAANAVFGGPRMGPIFGGMLLSGKKVADQIIKKLRGEYNENKVSKLTISCNR"
     gene            complement(4438..5241)
                     /gene="thiM"
                     /locus_tag="PLV81_03365"
     CDS             complement(4438..5241)
                     /gene="thiM"
                     /locus_tag="PLV81_03365"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOM09173.1"
                     /translation="MVTGNDARIILENVRLQKPLIHNITNYVVMNYTANALLACGASP
                     VMAHAYNEVQEMTGIANALVLNIGTLENEWIHSMIIAAGTAHKKGIPVILDPVGAGAT
                     SLRTHTALKILNTGAISVIRGNASEVLALTDNNAQTKGVDALHSVNDAIEVAMMIADK
                     YSAVVAITGPTDIVTDGKQIVKVHNGHKLLRMVTGTGCTATALIGAFCAVDNDYLKAT
                     ACALAFFGVAGELAAKKAIAPGSFMIKLIDELYAITPSELEQYANIENE"
     gene            complement(5255..6535)
                     /gene="thiC"
                     /locus_tag="PLV81_03370"
     CDS             complement(5255..6535)
                     /gene="thiC"
                     /locus_tag="PLV81_03370"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOM09174.1"
                     /translation="MTQLDFAKQGSITREMQQAAEYEGISAEELRNLIAKGWAVIPKN
                     IKRTFSVRAIGKGLLTKVNANIGTSTDHADIKEEMKKLEAAIYYGADSVMDLSTGGDL
                     HAIRKMVLEHSTVMVGAVPIYQVAAELSAKDKSILDMSENQLFDAIEQQCEEGIDYIT
                     VHCGVTQHSAELASKQRRVMGIVSRGGSLLAAWMRHHKKENPLYEHFDRLCAIAHKYD
                     VTLSLGDGLRPGAIADATDAAQIEELVILGQLVKKAREAGVQVIVEGPGHVPMDQITA
                     NIIAEKRICDNAPFYVLGPLTTDIAPGYDHIVGAIGGAIAALAGADFLCYVTPAEHLC
                     LPTVEDVRLGVIGTRIAAHSADIAKGIPKAIQQDFEMSQYRKALDWEGMFSKAIDPDM
                     ARARRKKSEDYEEKVCTMCGKLCAIKTHNECELL"
     regulatory      complement(6614..6715)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(6772..7113)
                     /locus_tag="PLV81_03375"
     CDS             complement(6772..7113)
                     /locus_tag="PLV81_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HOM09175.1"
                     /translation="MKKAKTFQDTLQFAQMVASKHGWVLHPDKEFLDILIEGLTINYN
                     RYGYYSCPCRDADGIRDQDKDIICPCDYCPPDIKEYGHCYCGLYNDPEFIKNGGMPKS
                     IPERRQYYFTK"
     gene            complement(7116..7421)
                     /locus_tag="PLV81_03380"
     CDS             complement(7116..7421)
                     /locus_tag="PLV81_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOM09176.1"
                     /translation="MIENITFTYRDGSHNAGNVTVYALSTCGHCKRALNFLDSNSVKY
                     RFVYVDLLPSEEKNKLKDYLVQKSNMYVAFPFLVIDDNTFFTGFIEEEWRRMLKLEK"
     gene            complement(7672..7854)
                     /locus_tag="PLV81_03385"
     CDS             complement(7672..7854)
                     /locus_tag="PLV81_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09177.1"
                     /translation="MDELILYLINEYHTAVMHTLTIATIIAVWVVICGCIRFGIAIYR
                     RAKGIYPPAESLREGH"
     gene            8215..8898
                     /locus_tag="PLV81_03390"
     CDS             8215..8898
                     /locus_tag="PLV81_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOM09178.1"
                     /translation="MSIIDNYRQLLQKIELIAKQCNRNPAEISVIAVSKTFPAQIIQE
                     AIDNGITLFGENRVQEAKAKIPQLQGNFTFHLVGHLQSNKAKDAVRLFECIHSIDKIS
                     TAIEVDKEAKKINKIQNILIEVNTSGEPSKFGVEPEKAVELCKAIRDLQNVTCTGLMT
                     VGPLTEDRNKIRDAFKQLRHLKEEINQTLGISLQELSMGMSGDFDIAIEEGATMLRIG
                     TAIFGQRIS"
     gene            8898..9701
                     /gene="proC"
                     /locus_tag="PLV81_03395"
     CDS             8898..9701
                     /gene="proC"
                     /locus_tag="PLV81_03395"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOM09179.1"
                     /translation="MDLGNISIGCIGLGNMGSAICIGLSKLLHSENIIGYDVDSSKVK
                     ELSQKVNLSAASSIQELSDKSDVIIIAVKPNMVETVAREIKSKQKSALVISIAAGITL
                     ARLSEWLGDSYRIIRCMPNTPALIGEGMSVLSTVSATENDIHIAQDIFSALGKVIILP
                     EHLMDAVTAVSGSGPAYVFTFIQAMADGGVKAGLSRNDALTLAAQTVLGAAKMVLQGE
                     EPIILRGKVTSPGGTTIDAIHVLEKNGFSGIVMDAIEAACSKSKKLGEK"
     gene            9701..10657
                     /locus_tag="PLV81_03400"
     CDS             9701..10657
                     /locus_tag="PLV81_03400"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOM09180.1"
                     /translation="MLTNYHIWQIKKAIRFMIPVIIAIFVIGNIIAFFIKDPIKVPDN
                     ALYKKNGLLLFKDASAYISLLEKYPYNPGVKLTIYTIREGESYWDVIQRYGISIDTFI
                     GANPQLDSLLAKAGTKIVIPHKDGLLIACKHFYEAWLAKLYTSGKGSISGRYIHHPLE
                     LLAMDDIRFAFIENARPVAVNSKIEKLYALRQKFQSPINGKFTSMYGDRVDPLEHGMA
                     FHNGLDICAPQGTPIKPVQSGFVNFTGWLDGYGFTIIIQHDEGYESWYGHCQKIFAQT
                     GEFVTTDTMIGTVGSTGRSTGPHLHFMLLQHGKMLNPIMFIW"
     gene            complement(10663..11394)
                     /locus_tag="PLV81_03405"
     CDS             complement(10663..11394)
                     /locus_tag="PLV81_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOM09181.1"
                     /translation="MRAIVLAAGTGSRLGEMTKNKTKGMVQVNGKPLIDYLFEFFDLN
                     FFDEIIVVGGFAFNDLEKHLKQKQIEHLKILENKEYHKGNIFTLITALKTFAHDSFLI
                     TNVDHIYPKVMFETMKQSFTRITAMCDFDRQLGPDDMKVKLDVDRKTIIHISKQLHDF
                     DCGYIGMTYVDRTMETVYRDSAYRAIERFGEKAVVENILQILAENPRTAPTICDLSGF
                     GWYEVDDEQDLIKAEKGLFQNPNFS"
     gene            complement(11376..12023)
                     /locus_tag="PLV81_03410"
     CDS             complement(11376..12023)
                     /locus_tag="PLV81_03410"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOM09182.1"
                     /translation="MIIQTKALLFDFDGTLVDTMEGFADIAGEVIHRFHPEISFEEAR
                     RRYLETSGVPFFQQLEIILPGNPTNSQKAAIFEETKKEGFFRSTFSQEVRYVINELRR
                     LGFIVGVSSNNFQYLIEQFINREGLQFDVVLGFRDGFEKGKQHFEYVIQHFNLQKDDL
                     TFVGDSLKDAEKAITNNIKFIGLCGTFTREQFLQKYPNIVTIESLKELLSCVQLY"
     gene            12263..12535
                     /locus_tag="PLV81_03415"
     CDS             12263..12535
                     /locus_tag="PLV81_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOM09183.1"
                     /translation="MNSDEYIKIELSETIDLHCFHPNDIKGILDEFLDNAYTKGYKKV
                     EIIHGKGKSVIKSIVHNYLKNDSRITDFFDKHGNWGTTIVILKIGE"
     gene            12537..13460
                     /locus_tag="PLV81_03420"
     CDS             12537..13460
                     /locus_tag="PLV81_03420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HOM09184.1"
                     /translation="MNTIIITGGTGFIGSNLTQKLLTKGYSVVILTRNPDRFLSHTPA
                     LTYAGWDGKTADGWGNLVNEAKAIINLAGESIASTRWTNEKKTKILQSRINAGNAIID
                     AIKKAKHYPEVLIQASAIGYYGSRSESLTETSSPGLGFLPDVAIQWEASTKEAIKYGI
                     RHVIIRTGLVLGENDGFLKILIPVFKWYLGGYFGDGSQWMSWIHIEDEVNAIIHCLEN
                     DAIKGPVNAVSPNPVQAKYFYKTLASVLRRPCLFKIPEFAIRLILGQMAEELILSSQK
                     VHPDVLISTKFNFTYANLYEALYSLLSKRSH"
     gene            13491..15884
                     /locus_tag="PLV81_03425"
     CDS             13491..15884
                     /locus_tag="PLV81_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOM09185.1"
                     /translation="MNNLPVITYDEAQMLEWHALLSYFRDFCSTETGKQYCINLQPLQ
                     YQDIIIQYNKINCLIEILRVGHSPDFSGIYDITPLLDKAAKGGTLLLDEIFKIRLFLV
                     AQHRITSFFRSVYYDISVLTELHAIDECIQLFRLLTTSLTENCNLNENTYPALRKIQK
                     DINEMRSIIEKNLTGIMHSQAYQHALQERIFTVKNNRYCIPVKSSHKGKMKGTVLDIS
                     SSGATLFIEPESIHELNNTLLYKEVELQREIDKILQILSSEIGTYAHELKHNATILAY
                     LDFCVACARFAITYNAHTPAVSPAPLLDCINARHPLLAILLRDAVVPCTIACGKSFNC
                     LIISGVNTGGKTVLLKMLGLFALMVRHGFPITANPDSTIGYFEHIFVDIGDEQNIMQS
                     LSTFSGQIKNIVRILNHANEKSLVLIDEIIVGTDPKQGAAIAQAVLEHLADKKSIIAV
                     TTHYSQLKEIASHDNRFENASVVFDIHTMKPTYQVLSGIPGASYTIEIAKNLSLPDTI
                     INKALSLLDATSQSIDALLSKITEQSHALQQKEQELSLLKEELQELQQEYNKKIMELQ
                     TTIEKVTREQATSFMNELQEYRNMIVNRIKEIQSMSMKDATTLIQDITQLQEKVKSTI
                     TSLHKPHENSVPCNTETAEKGVRVYIPSLQQYGTIEEVDTKEHTVKVRMGSIASRFSF
                     DDVFCIVESKKPVAIKKKTQAIKEEITSIPITIQTQYNTIDLRGLRVDEALQKLEAEL
                     DSMVKRNVSSAVVIHGHGTGSLKKAVRDYVKHSFYAKNFRPGEPEEGGDGVTIIQLR"
     gene            complement(15900..17486)
                     /locus_tag="PLV81_03430"
     CDS             complement(15900..17486)
                     /locus_tag="PLV81_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HOM09186.1"
                     /translation="MKKVWWKHGVIYQIYPRSFKDSNHDGIGDINGITECLDYLQDLG
                     IDGIWLSPIYRSPMYDFGYDVSDYCDIDPVFGTMRDFKHLVKEAEKRNIAIIMDMVFN
                     HTSHLHPWFLESRSSKDNPKRDWYIWHPGKKRKVPNNWMAAFGGRAWEWDPLTQEYYL
                     HLFTKQQPDLNWRNPNVKKAMFDILRFWMDNGVKGFRFDVINYIMKDPLFRNNPYKFR
                     LTYPRRHDLQYHLYDRNQPEIHDILKELRHLLDSYGQIMSVGETYPNEGEREPLMAAS
                     YLGNNDELHLSFDFSTIYISFSAKEFTSILIQWYNAIGNKGWPSHVLSNHDQSRAITR
                     IAKNDLQKAKLLAVLLLTQKGTPFIYYGEEIGMVDGKIPYRYIQDPVGKKYWPFHTGR
                     DKARTPMQWDATSYAGFSTAKPWLPVNPDYTRTNVALQSKNAESMMQFYRQLIALRKS
                     RKELYAGEIEFLPGSDDVMLYKRVWNNDTCLVALNFSSSKRKINFDSTCRVLLSSHQK
                     NIIDVQTITLDPFEAVICSK"
     gene            complement(17502..18698)
                     /locus_tag="PLV81_03435"
     CDS             complement(17502..18698)
                     /locus_tag="PLV81_03435"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOM09187.1"
                     /translation="MSILDLRPENFKKAFPDSKVMHVNGKSIIQSAMKEGNHWVVMAV
                     NTRHEIAIPGLIKASMELQAPVIYELALSESDLTGGYTGLTPKDFITLVVTLNEKMGN
                     VGEKAVPFTCHRDHTTVQEITRKAFDKADSLIKASIEAGYGSFSIDCSFLPMEQNIAL
                     SALLSEQVVRAGLMYESEVGEIGGAHGNSSVSEAVTLVEALAKLGQCPDLIAINNGTT
                     HGNVRGNIDLKLTYNTYKAMAPWGVGIAQHGTTGTAIEDVGKFYKYGIFKANVGTTWQ
                     NVIWGIDTDEYGVAVEKGPSYQKNPDKGISMDLWKKMEEYALSNNMKGGNMKKLFKPF
                     KDAMAQEYHSNAKIRERIDQAVYETAAAYFKALQAVGKADDVKAQMEKTVTKQEVKES
                     KEKDYE"
     gene            complement(18793..19626)
                     /locus_tag="PLV81_03440"
     CDS             complement(18793..19626)
                     /locus_tag="PLV81_03440"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOM09188.1"
                     /translation="MTTYPYPIIDFHVHLFPDRMFDAIWKSFVKDYGWDVMYHLYYKE
                     CIAYLKERGIEKIVYSNYAHKEGVAKHLNDWNLKVLDEQDNVYCFGAYHPDDDIAIAE
                     DFITHPKVLGFKLQLLVQKCYPYDTRLFPLYELVQHYKKRILMHVGTGPVGNEFVGIG
                     GFKKLLRQYPDIPVTIAHMGAYEYDEFLELLDDYPHLMMDTAFVFLPDYEGAFHKDPE
                     VLEKYQDRILYGSDFPNIIFPRELEIETLMKYGLSKTTLENILYNNGNRIIRQIVNAD
                     R"
     gene            complement(19623..>19791)
                     /locus_tag="PLV81_03445"
     CDS             complement(19623..>19791)
                     /locus_tag="PLV81_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004478830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOM09189.1"
                     /translation="SEAMKARYERSRIKGLDILSIGVAVGFNSNTTFCTVFSKMTGMS
                     PKQYRKEYGKK"
     assembly_gap    19792..19891
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19892..20686)
                     /locus_tag="PLV81_03450"
     CDS             complement(<19892..20686)
                     /locus_tag="PLV81_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09190.1"
                     /translation="MSSESVLHFFMFYGAVFSVIIALGQFVQKKIVTINKLYAVSFFG
                     LGIWLLQICIYSSGLFDSSAIGYYVSIASIPVIFIVPPLMVMRYTWIITSLFDVKKIY
                     YIVLLPSIISLFYLLVPLFIKGISCGSACFPGLPLLSDRFYELSLYDKGVYLLFIIPN
                     IYLITFMLPNLITMAYVWNRKKWNTVPGVSRMGYIFALSIVVSNAICVYGAFVSLHVV
                     EWAVIIANTAMTFVYLVTQRHPDYNKLLKSEAMKARYERSRIKGLDI"
     gene            20854..22695
                     /locus_tag="PLV81_03455"
     CDS             20854..22695
                     /locus_tag="PLV81_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOM09191.1"
                     /translation="MTQHQNVNYEKEFEEYLNREKHLALMVLNRAKKFGDSKIAVRHK
                     AYGTWEAFTWKQFGDLIESAARGLLLFGVKEFEFVGIFSNNRVEWAVVDYASFAIRAC
                     SVPVYATNSAKELEYIVNDCGIRILFVEKQEHYDKAMSIIDSAKSLEKIIVFDRNTKI
                     ASDPRVMLFDDFIAMGKSKSIYAQLQERLSKVQSDDLSTLIYTSGTTGEPKGVMLTHK
                     NWFAMLFGTGYHIPVEPTDVNLAFLPLSHVFERAWSYFILCNDAQVDYCHDTKALLDF
                     LKESRPHFMCSVPRMWEKVYATIMDGIKNAPPAKQNLFNWALEIGGKYQYLKKDHKPI
                     PIGLKLKHALATKLVLHKIRDLFGGRNKVFNCGGSAFSGEISEFFFKAGVFILQGYGM
                     TECFVICVSNPERNKFGTCGPVVPLMQVRVSPEGEIQAKGPSMTQGYWNKPELTRALF
                     TEDGWIKTGDVGFIDSEGFITITDRIKDLFKTSGGKYIAPQQIEMLFKEDIYFEQTAA
                     IGDGRKFVSALIVPAFEQLEKWAKNNNISFSSREDLVKHPEVIKFYRERIDYLTRDLG
                     QVEKIKKFTLLPHEFTQESGEMTPTQKIRRKVINEHYKDIIEAMYAE"
     gene            22823..23269
                     /locus_tag="PLV81_03460"
     CDS             22823..23269
                     /locus_tag="PLV81_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09192.1"
                     /translation="MYNSKTIITISFIFIATMALAQHQHGKGMMYHMYDPSSEVTVSG
                     TVEDTVTEVHPCCGSTMNHYYIKVKTNNETVKIFLGPTDYVKSQITIKKGDNVTILGS
                     KTTMNNETVIITKTVKTKDKEVTFRNDDGTPKWAGQMKYKKYKMNQ"
     gene            complement(23270..25879)
                     /locus_tag="PLV81_03465"
     CDS             complement(23270..25879)
                     /locus_tag="PLV81_03465"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOM09193.1"
                     /translation="MPEIKFDTIRKNIESNPLYGAAIGLILGLLIIGFLFTNVYRLFE
                     YKLFDVRFKIKPQLPQWEYLTFLDIDDNSISNVGQFPWPRYIYANGLEVIKDIAPKQI
                     AFDIQFMDKSPRYAQPDILKIVEEKVKTGKRITYEELNQAILDSDRLFSEAIAKNNVI
                     LAYSFLNRTLAETKLTPEEQQKRNESIQRFEKIASIPVPKDKQKEFSRYVDPTRVIIQ
                     YPIPELAMKARDFGYVDSDFDIDGIARRIRLIRVFNDRIYFHMAMVMLMDLMKVKKTD
                     VEIVHGKHIILHNAIDPITLQKKDYVIPIDDAGMSYIHWSGEFTETFNHVSFYALLEY
                     NQLKDTIHEFFDEQEVQSKNTERSRLYGELATMHEIFEKTKDMKAKQDLWEKIKTNRK
                     RLYEIESGYIKNLENQLAEINKQLKETNDNQLKEASKNLINFINAARLVLEVDRLKDH
                     SCIIGLTATASHDIGVTPLSSEYLMVGTYHNFINTVLQQAYIYQVPQWIDYCLILLLA
                     IFLVLAVQRLSAMGTITAIVGTLVVYNIINIALFAFQNLYMDQLGVSLAILLPSAIVS
                     SVKFMSEESQKRYIKEVFSKYMAPRVVEELIKDPGKLQLGGELRNISIFFSDVAGFSS
                     ISEKLTPQELVALLNEYLSEMTDIILKYEGTVDKYEGDAIIAFFGAPLAFEDHPERVC
                     LAAIDMKRRLAELREEWRKKGTFELKVRMGINTGEAVVGNMGSRQRMDYTMMGDAVNL
                     ASRLEGANKYYGTYTMISETTYERVKDVVEARELDLIRVVGKEQPIKVYELFGKKGTL
                     PDYMYEMLKKYNEGLQLFRERQWEEAQSAFKAGLRIVPDDGPCKTYIERCKEFAKNPP
                     PKKWDGVYRLKTK"
     gene            complement(26091..26702)
                     /locus_tag="PLV81_03470"
     CDS             complement(26091..26702)
                     /locus_tag="PLV81_03470"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOM09194.1"
                     /translation="MYISKNYYSLFPQLQKIIQLYEEVEKITKNVKNLFSVSCPHGCG
                     QCCKTASHNIEASIIEMLPLCVYLYEHTNYQLWLDKASDNLCPFYKAETLYDDAGCCA
                     VYEYRPLVCRLFGFSFMKNKYGAIVPVACSTLQNQYTAKKEIAAIHFSNLPHMSSLSM
                     QSMMIDPSRGERYPINEAFIKAMEYVILKIRLMHYQSEYNKTA"
     gene            complement(26792..27409)
                     /locus_tag="PLV81_03475"
     CDS             complement(26792..27409)
                     /locus_tag="PLV81_03475"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOM09195.1"
                     /translation="MKVSQLQTVLLHTCCGPCLTHVHSVLVSQYNIHIFYYNPNISPL
                     SEYKRRLYEVEQFSHLHSIPVTIGHYNQREWFNAVKPYRFYEELSPRCHACYAFRLEE
                     TFKRAREQNFDAVCTTLTVSPYKDAEKINEIGKTLSKVYNITYIPSDFKLDGGYQHSI
                     ELSKQYEMYRQKYCGCIYSKLERKKYSLWSKRVQAFKESFDALNK"
     gene            complement(27406..27762)
                     /locus_tag="PLV81_03480"
     CDS             complement(27406..27762)
                     /locus_tag="PLV81_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09196.1"
                     /translation="MKKRNKASFGVNGWYLSPSDNICLAKCIFDEPYREEIKEINKHE
                     IIVKIADCIGPEPSNLCIAEEIFEKLKFEIANFVIHHEYNCTRGVNADLLLWIDGKVN
                     PAKLKKLNSFIDKYLK"
     gene            28221..29105
                     /gene="aroF"
                     /locus_tag="PLV81_03485"
     CDS             28221..29105
                     /gene="aroF"
                     /locus_tag="PLV81_03485"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOM09197.1"
                     /translation="MATCNANPVSLSCNITNVATPSSPLYSKTYRDASVIHLPYNISI
                     SENTLAVIAGPCSIESEEQIMATAEFVKKSGAAILRGGAFKPRTSPYAFQGMGIAGLQ
                     LLRKAGDYYRMPIITEVLDSEDITIVADYADILQVGARNCQNFSLLRKLGKIRKPVLL
                     KRGMMVTIKEFLMAAEYILAEGNENVILCERGIRTFETETRNTLDISAVPVLKQKTHL
                     PVIVDPSHAAGNWKLIHPLALAALAAGADGLMIECHIKPESALCDGEQSLRPDKFALL
                     MNDIRKMNTLLNKKLECI"
     gene            complement(29102..30022)
                     /locus_tag="PLV81_03490"
     CDS             complement(29102..30022)
                     /locus_tag="PLV81_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOM09198.1"
                     /translation="MKKVGVIVNLNSRKYKLAKKHPEGVFEKIGQDSVIVKYTKSIEE
                     IVDVAREFKKTGIDYIAPSGGDGTLHHVITQFAKVYNNNLPPILILKGGTMNNVANSI
                     NLKGDAESILQRAVIAINNGKYINVVDRKTMVIDNNHCFLFGNGLVADFLETYYSIGK
                     SYTKLVGLIFSSIYEALANQQSHLFKGFEGNIKCDAESMPYSRVLGILAGTVETIGMG
                     FYPLYRANEHKDKFHTIVCAMKPGKLAKNVLKLKNGTPINHKDYIEKVVSTIEIHSTK
                     PFMYTMDGDLYRCDGSLIVSVGIRVPFIIV"
     gene            30208..30768
                     /locus_tag="PLV81_03495"
     CDS             30208..30768
                     /locus_tag="PLV81_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOM09199.1"
                     /translation="MAIICIRGGSLSAGHNVKTSYVDLLSHDGLLQTHTLINISKLGD
                     SSFEGVWQFDTVLLHKPDILILHFGIDDIYRPVYRSEFKENLVRMVQKARNASINNII
                     LTTLHLVRNQYDMDAVDVFTRTVREVALDMNCLLATVHIEWINYLFETGKSLNTLLTA
                     DDRYPNEKGHALIAEAIKKKLVQLLS"
     gene            complement(30825..31814)
                     /locus_tag="PLV81_03500"
     CDS             complement(30825..31814)
                     /locus_tag="PLV81_03500"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOM09200.1"
                     /translation="MIKTKITEMFGVKYPIICGAMMWLCKPKLCAAISNAGGIGNLTA
                     GNYNTEEEFRNAIRETRKLTDRPFFVNVTILPSVRITGEHHKMYLKVCAEEKVAGIEV
                     SGAPIDKACGMEYIDMLKKAGVKLFHKVGAVRHAVHAEHVGYDGIYAAGIEEGGHPLS
                     DDVTTMVLTTKIAQTVKIPVITVGGIANGKTMAAALMLGAEGVMMASRFIATKECEVH
                     DNIKQELVKRQEYETTLIMKSLHLQGRALKNEVVNKILEIEQRGGGFEELFPLISGER
                     MSKAWEQGDVNAAPLMVGQSIGLIDDIPTCAELLENMNKEAEEQIKKVTGNIK"
     gene            complement(31844..32992)
                     /locus_tag="PLV81_03505"
     CDS             complement(31844..32992)
                     /locus_tag="PLV81_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding domain-containing
                     protein"
                     /protein_id="HOM09201.1"
                     /translation="MDSQILKQLEGYDRVITDIDVLKRYGYDYRLERGLVKKYVERLH
                     PQIIDVKVIDIIEETKSTKTLRLASKQKYLPPFQAGQYISVIVEINGIRTSRPYSISS
                     SPDEIAYYDITVRRVEGGLVSNHLLDSLTIGDSLQISGPAGNFYHNPIIHGTKLVCIA
                     GGSGVTPFKSMIQHAVNTGTEREIWLIYGSKNADELIFDDYFAKVASKYKFFHYIPVL
                     EEGTGTYKGYITAKIIKEVVGDSSDKTFFVCGPQALYEHLLKELKPLTLKPRQLRMEM
                     YGVPSNVFEQPGWPKDISRNSTFVIKIRGKKDITVNAGEPILQSLEKNGIVVPSLCRS
                     GECSLCRVKLVSGKVYQPEGVKLRQADRRFGYIHSCAAFPISDCEIII"
     gene            complement(33003..34490)
                     /locus_tag="PLV81_03510"
     CDS             complement(33003..34490)
                     /locus_tag="PLV81_03510"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOM09202.1"
                     /translation="MYDVIIIGAGNAGLSAAATLARKGVKTLLIEKHNIPGGCATSFC
                     RGRFEFEVSLHQLSGLGTPERPGPLRSVLDSIGVLNKLEFVEMEKLYRVTLPGVIDVT
                     LPTNKEELIKVLQGKFPKEKDAIQKFFDFVWQFFTEVIGAFYLNDPDINPKKYPLYYK
                     YALKDTQSVLDEYFTDPILKAVISPYWTYMGLPPSKLCFSDMAALLFSYVEFKPYHIK
                     GGSQMLSNAMLEVVYENGGEVLFNTEVEKLLVKNNKVYGVRTSSGQEFESTYVLSNAS
                     AISTFVDMMDDAANHKVLEQYKGSSVGTSFFTIYCGLDCEYHEVGINEETNFISNTTD
                     MEFDYELSKTLETDKSSFILTCYNVFDNEMVPKGTSQVVLVTMKYAEPWLKVPPHQYN
                     DEKYRCAEAMLKNAEKVFPGLISHIEEMEIATPITHMRYLGHPGGGAYGFDQYVKDST
                     LFVSPKPPIDGLYCAGSWYGSAGYQPTLTSGHSAARAILKEMRNK"
     gene            complement(34630..35973)
                     /locus_tag="PLV81_03515"
     CDS             complement(34630..35973)
                     /locus_tag="PLV81_03515"
                     /inference="COORDINATES: protein motif:HMM:NF010635.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOM09203.1"
                     /translation="MEQILKKIEKLMLEHDAISPELIKKKDIKLGLRNPDGSGVVAGI
                     TSKGIVIGYEKIPNPDGTYTVKPVEGKLYYCGYDACELAQRIQEEGRYGFNEITYLLL
                     TGELPNKKDLQRFEEVIADRRPLTKLERSIIMQEDVNYNQMYALHSVISHLSRCDPDP
                     DSTDIKDVSRQCINIIAKAPVIIAYNYNVMRFRKGGDLFITKPNPAWSTAENFLYLLR
                     GERPDEYEAKLFDLALMLHAEHGGGNNSTFAVRTVTSSGANTYMALCAGVASLSGHLH
                     GGANEAVMKMMKDIKQHVKDWNDDEEVEHYLKLMLEKKAYDRSGKIYGIGHAVYTKSD
                     PRAIIFRQKAEEFAKMKKNLDEFNLFAKVADIGSRLVQKYKGTVASPNVDFYSGLIYK
                     MMGIPMELFTPIFAMARFSGWSAHRIEQIIHDKIIRPAYASSLDEFKEYIPLEKR"
     gene            complement(36239..38206)
                     /locus_tag="PLV81_03520"
     CDS             complement(36239..38206)
                     /locus_tag="PLV81_03520"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOM09204.1"
                     /translation="MEWIVSKNKKELKIQLKKPDVLFESWKIQYIYSIHNQSIHRIQS
                     LPVTALGKKKTTCIINDDNLRITVVISDEVDHLAIKYTIDNLSDEDLSLDKIITSIGR
                     FSIPKTEKYYVFKNGFQSWSLTRSYSFDEKEMVSWLTCMNILQDTIRNLPSGKNGSFN
                     STMFIAIGSENSSYCVIGQGRPFNQYVYINTLTTGNTIQLDIVHDFCKVIVSPALKLR
                     ADLIYFITNKNINEAFDRYFELIRSQIPIRRKIPKGWCSWYYYYTKINEKSIYHNLDV
                     LRSKGKRFDVFQIDDGYQTEIGDWLSINNKFPGGLAPIARKIRTMGMVPGIWLAPFIA
                     SRKSMLYQQHPDWFLHDSDGKPVIAGWNPNWDIFGYFYALDTTHPEFQDYIKEVINTF
                     VHEWGFGYLKLDFVYAASLCGRAYDMSLTSAQRLKLGYSLIRDVAGERVFILGCGAPL
                     SASIGHVDGMRIGPDVAPYWMAKYRYYLTRDPHALCTKFAIRSILNRSQMHRYLWLND
                     PDCIMLRQTETKLSKEERMTLINAVIITGGMYFISDNLALLHNDDWKLIDTIDELIRI
                     CSLEKSYPVDIMENDIPEIIYNSAGYIAFFNFSDSSRKKIVQLKGLLKKILTSASKLS
                     DVWGNREIYVYSDHLDLGELKPHESVLCRIK"
     gene            complement(38216..39256)
                     /locus_tag="PLV81_03525"
     CDS             complement(38216..39256)
                     /locus_tag="PLV81_03525"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOM09205.1"
                     /translation="MKKSIIIIIVLILIVIAFVIKTLYDAGEFKTITSLSNYSCKPVG
                     SIPGPEDIVIDYNSGIAYISSDDRRAYARGQNINGAIYSYNTKTKVLNKMKTDFPFEF
                     HPHGIDLIAITKDKLLLYVVNHRTDGHYIEKFLIKENTLKYIKSIEDQNLMFSPNDCA
                     MVAEDMFYVTNDHGNKSAFGKSLEEYLQLSNSYVLFYNGKNFEIKAEDIKYANGVTLS
                     PDRLKLFVASPVGKCIKVYTIEKGYNLTFLYDIQCNTGVDNITFDRQGMLWAGCHPKL
                     LTFVKHSKNEQAIAPSEIIKITFTDSGYKKETEFLDDGKLIQSSTVGVRYNSSLLIGS
                     VFDNHILDCSSK"
     gene            complement(39480..40193)
                     /locus_tag="PLV81_03530"
     CDS             complement(39480..40193)
                     /locus_tag="PLV81_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09206.1"
                     /translation="MNIEDNIKKAQTIYRAGLENNGQSLNLLQELLACNVTDYIVKAG
                     KDWIYFDVSLAMEYFIKNNDIDGLYHAGIYWKNFDYQRGINQIFEWDNDEYIFRAGRF
                     WKQFDYERGLARLIELHSSKYIYHAGLDWKSFNHKIGFDALLNIGDPEYIFYAGMHWV
                     YFDYEKASEVLIKIENCECIYKAGCQWKWFDYEHGWQILERNVIEGRKWRGKALENER
                     WKKGLKEMWEGMKKDVNKI"
     gene            complement(40232..41620)
                     /locus_tag="PLV81_03535"
     CDS             complement(40232..41620)
                     /locus_tag="PLV81_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09207.1"
                     /translation="MDNSFINSALRKIWKQSRFTSYFYHAVEFVHTQSIPTLSLVASQ
                     RMVLYYNPEFLNTISQDEFIGLLVHEMFHVILEHDHRAKTGDIVLQNIAQDMVVNTFI
                     SNHSKTFFSHKGQYQWDVPVLTIPQGLPFIPQEFYDETAIYDPVWEDVYRWLLQKQNE
                     LKVIQHHGDIEKQKGMFHDDRYAIAHNNSQEHDKEFNYKNYLSHSSVDDDKIILKDEN
                     SHLPTGVHYFAENNENKRSVKNKIIAHAKKDEWCTVERSYLDIVALLEKINMCDSSFY
                     KNHIKSIIDYLSHSNEWYYTHSRFNRRYVAKGIYIAGRAYKQSEIITVAIDISASMMA
                     QREDLETAFGIIEDLTGKYKVFLVCVDEEYFIPVKQGDTFIRSEPSAKPYEYKKGDWK
                     LIKSGYGGATFFSSFFNEYLKGHKEMVLVITDGYIYDVDKLKPYPSTLWLITKSRNEN
                     FVPPFGKAISIV"
     gene            complement(41626..42783)
                     /locus_tag="PLV81_03540"
     CDS             complement(41626..42783)
                     /locus_tag="PLV81_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09208.1"
                     /translation="MKRPVEVSTIELIEILDRYLQTEGAINYAIKIVGYPGVGKSAIV
                     EQVAKKHNYYYIDTRLAFKENVDLGGYPVPDNNLKRMIYFRPRFIPPETVPEGYNGIL
                     WFLDEANRAHPTVIQTLFQIITERMCGEHILPPKTAIVLAGNLGDSDNTVITDFDDSA
                     LDGRLAIFNLIPDASNWLQWAIEEGIHPAVIQYISTYPDRLWDSSAVNPNPRGWHQVS
                     DTLYKAYNFQTEEQFRIYLLNNDYKVLQKIIISLIGDVAGTDFILQYVSPRAISSNDI
                     LAGDEKKLQQLIDNTIPTEDILWAVNGAIHILYTENVKRSGSPDEQLLKKLSHFLKFI
                     SFTRADTRVSFMYSLVRDCGLFTQIPDALNYIDNPEEKQSIMEKVQVFLDV"
     gene            complement(42829..43941)
                     /locus_tag="PLV81_03545"
     CDS             complement(42829..43941)
                     /locus_tag="PLV81_03545"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOM09209.1"
                     /translation="MLFLSFVFFSSEVFAKTDKKDTVTGEPLQDIVVISQNDWLIIPH
                     LYYKPETQFAYGVVFLTYFQDIIMSIPPIKLRPTTLAITFTHTQNDQFIFQVFPEFYF
                     FRDKYHIKNEFQYMNYPDKFYGIGQDTTENDREEYTSRIFSFKNIIERAFIPGFYIGL
                     LYHIDVRKTTQAQDGGLIDSNLLPGADKGLVSGLGFTINYDNRDDILFTRDGSYANFT
                     ITQYSKYIKSDFDYINVTFDYRFFEPFWFSHVLAVQFISGYIHGQAPFYLLSQMGGAK
                     LMRGLYEGRYRDRNVIVVHAEYRFPLFWRFIGCGFAGAGEVAQTIREFNKEHIIYTWG
                     GGLRFKLKKDQTLNARLDIGFSKNFTGVYVTIGEAI"
     gene            complement(44062..45429)
                     /locus_tag="PLV81_03550"
     CDS             complement(44062..45429)
                     /locus_tag="PLV81_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOM09210.1"
                     /translation="MHINQNKNFTEDTNLTRGLGLPSAVSIVISRIIGSGIFRTPGPI
                     MALVGSTMLFGSVWIIGAVITIFAAVCYAELVAMMPRSGGPYIYLKEAYGPLIAFLRG
                     WAMFFVSETGAIAAVALVFAEYTQASLEISSGIYLSHAMVVIIALLIIVFHTFINCFG
                     VRLSGIVQIIISSSKIIALGYIITACFTKNGNVSNFYTPLLPEHFTLTHVLGIGAALR
                     YAFFAFSGWEGATYVAEEVKNPRKNLPMSLLIGIACVLILYLSANAAYLFQVPVDKFA
                     ISKWIAVDALQTALGATGALLVAYAVMINTFGNVGTQVMVKARTWQAMAQDNLFFSWC
                     AHVHTTYKTPNNAMIMQGVWACVLVLFASIYKNSYEAVIDYFSATGTIFNILTLTSVI
                     VLRRKYPEAVRPFKTWGYPVTVILVVLFYVIYLIVTLITAFMPSLLGIILTSTGLIYY
                     WIKIK"
     gene            complement(45435..46028)
                     /locus_tag="PLV81_03555"
     CDS             complement(45435..46028)
                     /locus_tag="PLV81_03555"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOM09211.1"
                     /translation="MAKDQSEKTVIDTRGYSCPISVALIAKTMKTVSDGSSVEIISDD
                     PSMKKEIEQWCVESGNIMNTVVVDHLTARFEIIKGKGYKPQSLCDLIAFYLLGVRLHI
                     RKIFVSLFRKDTTQCLITFVSVAEGMRAHYWLQNNHPELSYALLPVPNSITRHCGVVI
                     GLSSLKQARELFDLLRSNDFKVEDIYIKHNYTYHPAQ"
     gene            complement(46055..46849)
                     /locus_tag="PLV81_03560"
     CDS             complement(46055..46849)
                     /locus_tag="PLV81_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOM09212.1"
                     /translation="MEQDLKMMYGRLKRWSNGTDYYLESLNYKWAFTKKIIKVAIVFH
                     IAFVLAMGALLSYYSHHNPYTTPLMAYRLYVDGYKVKPVKFIPFQKIPDDIKKMVVSI
                     EDYKFYKHNGIDIEAIKRAIVINYKVGYRMYGASTITQQLSRTLFLLPAKSIIRKYVE
                     LLIALEMEMILDKDRILELYLNYCELGKGVFGIKNASYYYFGRNIYQLSTDEKSRLLA
                     ILANPILYSPYDFKNSKLITNRYYILKFRYYTYNKYRSMLQYAYHD"
     gene            complement(46800..47780)
                     /locus_tag="PLV81_03565"
     CDS             complement(46800..47780)
                     /locus_tag="PLV81_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HOM09213.1"
                     /translation="MNFKILYKYGVTVLLITGLIVFATSLLIGFNEKESSFTRYLLHA
                     PFSGRAGHTCTVFKGKLWLIGGWDGNNPYNDVWYSKEGLHWYSAGPMPFKPRAGHTAF
                     VYNDKLYIVGGLTFDAARNIIDLNDIWATEDGISWQCVKETAEFMPRGGHACTVFNNT
                     ILLVGGIACGAEAFKSDDGINWHKIAGNLPFGSRGGHVLTAFNNKLWVIGGIRVDGNN
                     NFTSFSDVWNSYDGIHWKKVASNMHFFAGGGHGAVVYKKSLWVIGGFRKGGTVYRSDN
                     GKDWELFNSFTPMGERVSHSCTVFKDRIILIGGFDGTGFKNDVWAFKTLE"
     gene            complement(47813..49285)
                     /locus_tag="PLV81_03570"
     CDS             complement(47813..49285)
                     /locus_tag="PLV81_03570"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOM09214.1"
                     /translation="MNTILEKYYRVLIADDEKGIRDLLKDLLEGSFYVSTIDRGDTII
                     EEITKNKYDILILDLQMPGKNGIEVLQTIRDLKLDIVVVVITASNDVQLAITSMKLGA
                     YDYLVKPFDTDKLLVILKNIVEKLDLENEVNELREKVGESFRFRNIIGKSPKMQRIFA
                     TLERVIDTDSTILIIGESGTGKEIIAKAIHYNSNRKQYPFKSIDCSTIPQDLIGSELF
                     GHEKGAFTGAIARKIGKFEVASKGTLFLDEISNLSFDMQAKLLRVLQEKEFERIGGNE
                     IIKVDTRIVAASNRDIREMVKQGTFREDLYYRLNVVPIYLPPLKERKEDIPLFIDYFL
                     NKFTTEYGRDISLDLQSRLYLTDYHWPGNVRQLENVIKRLVLLSTEHVVKLDIVKSVL
                     EFEEYNKPKEKTVNVENEHINHTIPEAFVNSKKVQDSESHVDRVKTLDEMEREYIEKM
                     LKHFDYNISLTAKAINVSRKTMHNKLKKYNIAIKKTVIEN"
     gene            complement(49317..49940)
                     /locus_tag="PLV81_03575"
     CDS             complement(49317..49940)
                     /locus_tag="PLV81_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOM09215.1"
                     /translation="MHEVDIIERVIRNIAHEVRNPLTTVKGYAQLLSQKNEPALIVKS
                     QHMIIEQSERIDTIFTQLYDAFTVIDDDRAECTFPEVLYTVVEESPYSEYITIERNAQ
                     FRISLYVNRFQQCLSSIINGFNWKYFSGASLHINALKDMHCIVNISFSGVVFDSIPED
                     IFYYPFQCKQFFSKGTELFKVYCIAQRSGWQFTFLKERNGFTLEIPM"
     gene            complement(49948..50331)
                     /gene="rsfS"
                     /locus_tag="PLV81_03580"
     CDS             complement(49948..50331)
                     /gene="rsfS"
                     /locus_tag="PLV81_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOM09216.1"
                     /translation="MTGKSKRTKSKITVDPEVLSLMKGCKKVLDDTKAIDIVFLDLRD
                     VNSYLDYFLICTGNSILHCKALAREVHKYFLEKGIKERSRTTTNSPWIVLDYNDIIIH
                     IFTKETREFYQLERLWADAKQIELD"
     gene            complement(50328..51434)
                     /locus_tag="PLV81_03585"
     CDS             complement(50328..51434)
                     /locus_tag="PLV81_03585"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HOM09217.1"
                     /translation="MKDKIRLIVSITLICIVVMGIFYVYRRFTRNAIETLAANKELIN
                     ILVTASNEYNERRHSFYAILHFNPEKKSIGVTFVPPNYLVTLNKRTGKAKRLESLSFS
                     DYSDIIDSLEDDLHITIPFYIELYASDAIKIIDLIEGINLYILDQVKMDLGYTTGINY
                     CDGRKALWYINKVEQNSIYLKYDRIMDVALTLFTNKDVYKKYAKKEYISEMVSSINTN
                     LLLQEIISLIDVVYDARTVYTTIMPGKMDKDNNYTVDDITRKLYEQKFLRPLVLDIKD
                     SESIKVRILNGSDIPGLARKMRNVLTREGLTVVEFGTSPYEVTDKTVIINQKGNLNSA
                     LKVSSILGIDTMYHIVDSSQLADVLVIVGKDYSQ"
     gene            complement(51424..52044)
                     /gene="nadD"
                     /locus_tag="PLV81_03590"
     CDS             complement(51424..52044)
                     /gene="nadD"
                     /locus_tag="PLV81_03590"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOM09218.1"
                     /translation="MNQIRLEGEAHLKVGIFGGTFNPVHIGHLINAQFIREEFNLDFI
                     VFIPTKYPVHKELEDNVSAEHRYQMIRLAIEGVDYFKVSRLEIDREKPSYTIYTVEEI
                     LQKNPDWELFFIIGYDAFNEINTWKEWKRLLMTIDFIIMKRPGDVLHKRFNKYMSRIH
                     CAKNPVIEVSSSIIRDRVRNGKSIKYLLPGRVEQYIYKHGLYKSER"
     gene            complement(52080..52508)
                     /locus_tag="PLV81_03595"
     CDS             complement(52080..52508)
                     /locus_tag="PLV81_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09219.1"
                     /translation="MAAGFILGKLLVAKTVSGSTAISFFITQPLYTYSAINNKLYSNN
                     PIERLTGYCALYELHIIDHPFLFERYKQEENIASKRVILNILALYGGKEFLQFFDEVY
                     ELSDKTLKKQMVKIVKHHYPEKLDSFAQKHKVDAQWIHTD"
     gene            complement(52669..53148)
                     /locus_tag="PLV81_03600"
     CDS             complement(52669..53148)
                     /locus_tag="PLV81_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09220.1"
                     /translation="MKKSIVCLTVMLLMMYVSFGQSQHMHQGHEGGKSTHKAMGTMVF
                     QAQKDGLSIEVYLNDVKTAMEQMIKESGMKFDASKLDPDITHHMSVFVEGKDAGTIKS
                     ATVDIKSKNISKTYTLFHMKNHYGSDVSLKNKGNYEAIITFNTEKKKGLQFRFTFSI"
BASE COUNT        16835 a        10472 c         8535 g        17373 t
ORIGIN      
        1 acacagaccc tctatctgaa gataatgttg tagtctttgc aaccggaccc tttactggtg
       61 ggcatgtatg gggtggtagc cgcggtcatt gtgccagcat atcccctctt actaacatat
      121 tgtgtgattc aaactttggt ggaaactttg cagcaatgtt caaacgaact gcctttgatg
      181 caatagttat taagggcaaa gcttcttcac cggtatatat acatattgat aatgagatag
      241 ttactataaa agatgccact tcactatggg gcaaaacagt ttctgaagca cactcactat
      301 tatcaaaaca aaacgagaaa agaattgaat cagcattaat tggccctgca ggtgaaaatc
      361 ttgtacggta tgcttctgtt atatgcagtg gaacacgtgt gagtgctgct ggccgtgggg
      421 gcattggtgc agtattggga gcaaagttat gcaaaggcat agtagtttct ggtgaaaaaa
      481 aaattgaaat ttttgatgta gataaactta aaaattgcct taatcaacat tatccgggca
      541 tgaaagaacg tgccaaacat ttaacagaat ttggcacacc ggtactggta cagatgatca
      601 atagcagggg aaaattatgt actcgaaata accagaaaga acaatttgat aaggcttatg
      661 tgataagtgg tgaatacatc aaagaacatc ataagaaaaa aaatattgcc tgccacggct
      721 gtccagttgc ttgtggcaaa cttgttgatg taccctatgg cacctatacc ggtaccaatg
      781 taaagatgcc cgaatatgaa acaatctatg catttggatc catgttagaa aatagcgatg
      841 ttgtttcaat ttacaatgct aatacagtct gtgatgaaat gggcatggat actataagtt
      901 ttggtgttac tcttgcattt ctaactgagt gtgttgaaaa agggctgata aaaaactcag
      961 acttagaaat accactgcat tttgggaaat ggaatcatgt agcagacatt gctgaatatg
     1021 ctgcatatca aaaaggtctt ctgggtaaat tactggcaat gggttccaga cgattatctg
     1081 aatacattgg tggcgaatca tataaatatc tttatgaggt aaaaggattg gagatagctg
     1141 gccactctgc tcgtggtcta cgacagatga gtctgggata tgcaactgca acacgtggtg
     1201 gaagccatca tgatacacga ccgacctatt atcctaatga ccctgaagta gatccaggat
     1261 ttactcatca acctgaatat tcctttaatt cacagaacaa taccgctatt ggcgattctc
     1321 tggtattatg ccgatttatt catgaacgca gttttggcac acagattaac gaaacattag
     1381 ctgaggcatt atcatacgtt acaggatggg atataacaaa acaggaatta gtagaaattg
     1441 gtgaacgaat ttacacatta gaacgcctta tcaacgtatc tcgtgggatt gatagacaaa
     1501 gtgatgtatt gccctatcgt gtaatgaacg aacccatccc tgatgggcca tcaaaaggct
     1561 ggatgtgtac taacgaagat ctgcaaaaaa tgcttgatga gtattataga ttacgtggat
     1621 gggataaaaa tggtatacca acacaggaaa cattaaagcg tcttaaaatt tattgattct
     1681 cattattttg acaaaaccat ttataacaca acgtagatct tgaatcaagt tcatgatgac
     1741 catagacaca tattgttata gttgtatact tacctgcacc aaaaatatga gtatcttacg
     1801 caaagtgatc ccagcctttt ggttttatat cactgaatgt accatcgttc cttttcatca
     1861 gatatttttt ttcttttgta attgtaccaa ttctgtataa tgtccttttg aaggttttat
     1921 aaaaatcatt ttctagtttt gttgctttct ttgcatcagc agtgcatact aatacataat
     1981 cttcaccacc ggcaagcgca tactctatgg cactatagcc atgttcttta caatagtgca
     2041 ataaatcagg tgatactggt attgcatctt cttgcagtat aaaacccaca ttactttctt
     2101 ctgctatatg cattaaatcc gaactcaatc catcacttat atcaatgcat gagtgaacta
     2161 caccagagtt tgccaaaaac aaaccttctt ctatatgtgg acgcggcaaa caatgtgctt
     2221 caaacagtct tttttcttga acattttttg gttgttctga tttaagaatg aaatccaatc
     2281 ctgctttact atcgcccaat aaaccagtac aaaatattac atccccctct tttgccgtgt
     2341 tgcggtataa gagtgatttt tttttggcaa ctcctgtaac taccacgttg ataattaaat
     2401 caacaatgga tgatgtggta tcgcctccaa ggatattaac attatattgc tttgcaagat
     2461 ctttcattcc tctgtaaatt tcatcaaggt attcaatgga tgtttcttta ggaataccta
     2521 tgcttacaaa ggcatgttgc ggaacagccc ccattgcagc aatatcgctt aaatttactg
     2581 caagcgattt atatccaagc tgataacctg tcacagcttc tttaataaaa tgtacgcgtt
     2641 caactagcag atcagtggta agaacaacac actcattttt gccaattgca aatactgctg
     2701 catcatcacc aatagctcgc attacattta ctggattatt gacactatct ggagtaatgc
     2761 gttttataaa gccaaattcg ccaatatctt taaggttcat ttgatttttc ctgatcacga
     2821 tattttaata acaactttta acctcacaga ttattgtctt taatgttttt gctgcctcat
     2881 agggattatc agatgaaaca atagctgaaa caaccgctat cccatctgca ccatgctgta
     2941 taacctgctc agcattatga atctgaattc caccaatggc aatgacggga atactcactg
     3001 cctttttaat ttcctgtatt ccctccagac caataattcc tgatatatca tcctttgttg
     3061 atgttgggaa cacagatccg gcaccaatgt aatctgcacc atccttttca gcttcaattg
     3121 cttcattaac agttgatact gatatgccaa taaccattgc atgcgggact atctttcgtg
     3181 caattgtcag tggcatgtca tcctgcccta aatgaacacc atacgcgtgt accgccaatg
     3241 ccacatcaat tctatcattg attattaaag gaacattgta cctgtcggta atgtatttaa
     3301 tcatttgggc tatttcaaca aattccttcg tatcaatatt tttttcacgc aattgcacac
     3361 aatcggcacc acctttgatt gcttcttcaa caacaacttc tacaggcttt tttgcaagct
     3421 gcctatcggt tacaagatat agtaagcttt gaaaccttgt tttcattgta ttcccctctc
     3481 aattttttga taatctgatc agcaactttt ttacctgaca atagcattcc accaaatatt
     3541 gggcccatgc gcggtccgcc aaacaccgca tttgcagcca taccagcaac aaaaagacca
     3601 gggaatattt cgcgcgtatg ttccagtgtg aacttttcag ctttatcagc ccacatggat
     3661 ttttcgcctt caattgttcc tgaaggagta ttaagcttgc caggaacttt tttcacaaca
     3721 actttgacca cctctgtatc atgacctgta gtatctataa catacctgga ttgcactgtc
     3781 aatggatcaa catggagttt agcaatatct actgctgacc agtttataac cagtccacaa
     3841 acacaatctt cccgcatcat aacatcttca acgcttatac agttaaaaat agttaatcca
     3901 gctttaattg cttttgatgc tattgtacat actgattcaa ttgcatctgc ggtatagtaa
     3961 ttttcagcat attgtgtata tcgaacatca aattcttcca gtataggcaa tgcatctttc
     4021 tgaacaacta tttcattaaa ctgcatacca ccgccccaca tgccaccacc aatggatagc
     4081 ttacgctcaa acaggatagt ttttatattt tgttttgaca gatagtaacc ggcaacaagc
     4141 cctgacgggc ctccaccaac aatagcaaca tcgctgacaa gggagttttt caatcgctga
     4201 aagtagcgat caataattgc cgatgtaatg gatacttcat tgagttccat agatatgtcc
     4261 tccataaaaa taggtaatgt atttcacaaa gttattcctg tgaaattctt gcttttggta
     4321 aaacaaagta gcgtattaat taaattgaaa attagattct tcgtcccgat acaatcggga
     4381 ctcagaatga ccatcagaat aaccagaagt gcattataaa cgaaatacca atcattatca
     4441 ttcattttca atgtttgcat actgctccag ttctgatgga gtaattgcat ataactcatc
     4501 aatcagttta atcataaaac ttcctggcgc aattgctttt ttggctgcta actcacctgc
     4561 aacaccaaaa aatgctaatg cacaggctgt tgctttaagg taatcgttat caactgcaca
     4621 gaatgcacct attaacgctg ttgctgtaca acctgtgcca gtaaccatac gtaagagttt
     4681 gtgcccatta tgcactttta ctatttgttt accatcagtg acaatatcag ttggtcctgt
     4741 tatagcaact actgctgaat acttgtcagc tatcatcatt gccacctcaa tagcatcatt
     4801 aacactatgt aaggcatcaa caccttttgt ctgtgcattg ttgtcagtca gtgcaagcac
     4861 ctctgaggca ttcccacgaa tgactgatat agcacccgtg ttcaatattt ttaatgctgt
     4921 gtgcgttcgt aacgatgttg cacctgcacc aacagggtct aatattaccg gtataccttt
     4981 tttatgtgca gtacccgcag caattatcat tgaatgaatc cattcatttt ccagtgtccc
     5041 aatattaagt accagtgcat ttgcaatgcc ggtcatttcc tgtacttcat tatatgcatg
     5101 tgccatcacc ggtgaagcac cgcaggcaag caatgcattt gcagtgtaat tcattaccac
     5161 ataattagtg atgttatgta tcagcggttt ttgtaatcgg acgttttcaa gaattatacg
     5221 tgcatcgtta cctgttacca tttattcccc ctttttataa taattcacac tcattatgtg
     5281 tcttgattgc acacagcttg ccacacatgg tacacacctt ttcttcataa tcctcacttt
     5341 ttttcctgcg tgcacgagcc atatctggat caattgcttt gctgaacata ccctcccaat
     5401 ccagcgcctt tctgtattgt gacatttcaa aatcctgttg tattgctttt ggaatgccct
     5461 tagcaatatc agctgagtgt gcagcaatgc gagtgcctat cacacccaat cgtacatctt
     5521 ccactgttgg caggcataaa tgctcagctg gcgtcacata gcacaaaaag tcggcacctg
     5581 caagtgcagc gatagctcca ccaatagccc ctacgatatg gtcatatcct ggagcgatat
     5641 cagttgttaa tggccccagt acataaaatg gtgcattgtc acagatacgc ttttctgcaa
     5701 tgatgtttgc agttatttga tccatgggaa catggcctgg gccttcaaca atgacctgta
     5761 caccagcttc acgtgctttt tttacaagct gtcctaatat taccagttct tctatctgtg
     5821 cagcatcagt agcatctgca atggctcctg gacgcaatcc atcgcctaaa cttaaggtca
     5881 catcatactt gtgagctatg gcacataacc tgtcaaaatg ctcatacaat ggattttctt
     5941 ttttatgatg tctcatccat gctgctaaaa gtgacccacc acggctaaca attcccataa
     6001 cacgtctttg ctttgatgct aactcagcac tatgttgtgt cacaccgcaa tgaacagtaa
     6061 tataatcaat gccttcttca cactgctgtt ctattgcatc aaaaagctgg ttttctgaca
     6121 tgtccagaat tgatttatcc tttgccgaaa gctctgcagc tacctgataa ataggtacag
     6181 cacctaccat taccgttgaa tgctccagaa ccatttttct aattgcatgt aaatcaccac
     6241 ccgttgaaag gtccatcaca ctgtcagcgc cataataaat agctgcttct agctttttca
     6301 tttcttcttt aatatcagca tggtcagttg atgttcctat atttgcattt actttggtaa
     6361 gcaaaccttt cccaatggca cgcacagaaa atgtacgttt aatgtttttt ggtatcactg
     6421 cccaaccttt ggctattaaa ttacgtaact cttcagccga tatcccttca tattccgccg
     6481 cttgctgcat ctctctggtg atactaccct gttttgcaaa atctaactgt gtcataatgg
     6541 taaccctcat aacaaaaaac cgcccagcgt gtgggcggtt ttttaagttt ttaaaaataa
     6601 aatgaaaaca gaagcaatta taaaaactac tccctccgct ggcattaccc agatcaggtt
     6661 ctaagggtat actctcaggc ctgtgcactt gcacaaacca cccctgcttt aaagaacttt
     6721 agtaaatgca atataataac agttatgtaa aaaatcaagt aaatagaaaa attatttcgt
     6781 aaaataatat tgtcttcgtt cgggtatact ttttggcatt cccccgtttt taataaattc
     6841 aggatcatta tatagcccac aataacagtg tccatattct ttaatgtcag gtgggcagta
     6901 atcacacggg catattatat ctttatcttg atcacgaata ccatcagcat ccctgcacgg
     6961 acaggagtaa tacccataac ggttataatt tattgttagt ccttctatta atatatcaag
     7021 aaactcttta tctggatgta gaacccaccc gtgcttggat gctaccattt gtgcaaactg
     7081 taacgtatcc tggaatgttt tagctttttt catggctatt tctccaattt taacatacgc
     7141 cgccattctt cttcaataaa gcctgtgaaa aatgtattgt catcaataac cagaaaagga
     7201 aatgcaacat acatatttga tttctgaaca agataatctt ttaatttatt tttttcctct
     7261 gaaggcaata aatccacata cacaaagcgg tatttaaccg aattgctatc aagaaaattc
     7321 aatgcgcgtt tacaatgccc acatgttgaa agagcatata cggtaacatt ccccgcatta
     7381 tgtgagccat ccctgtatgt aaaagttata ttttctatca tatgcatctc cttaaagaat
     7441 tgtatcacat cattttttat tcctgcttca tatggaaaca gttctgtata ttactataat
     7501 tgtataaaaa tatacaatct caatcaaaca atgtcaatta atattatttc gattatgttt
     7561 tatacatatc tgttaaagaa aaaaaatgat tgtaattaat aaaaaaactg cccgtaaagg
     7621 cagctttctt tagtatattt taaaaattat gcaaaatgac aaaaataagt atcaatggcc
     7681 ttcccgcaaa ctttctgctg gtggataaat acctttagct cttctgtata ttgcaatgcc
     7741 aaaacgtata caaccacata ttacaaccca cactgcaatt attgtagcga tagttaatgt
     7801 atgcataact gcagtatgat attcattaat aagatacaat attagttcat ccattgtgca
     7861 cctcaaaaaa agttggtttt tacaataatt acaatattac tgtgaaagta tgcataaata
     7921 ttgtcaatat aaaatttttt attttaaaaa aaattatttt tattcactca tcctggcacg
     7981 ttgtacagac aatataactt gtattaattc gtttctaaat attacaaatc atataacact
     8041 tttttcccat ccacctgccg tatactctca tagtgaaaac aaaaactgtt tgactttatt
     8101 tatttgtatg gtattttctg ctttaagcct ttaaatagct actatcgtta aaatatttac
     8161 ctgaaaattc ttatcaatat attttttatt aattgaggta atgatactac aggaatgagc
     8221 attattgata actacagaca attattacaa aaaattgagc tcattgcaaa gcaatgcaat
     8281 agaaatcctg ctgagatttc cgttattgca gtaagcaaaa catttccagc acaaatcatt
     8341 caggaagcca ttgacaatgg aataacacta tttggcgaaa accgagttca ggaagcaaag
     8401 gcaaaaattc cacagttaca gggcaatttt acctttcacc ttgttggtca cctgcaatca
     8461 aataaagcaa aagatgccgt taggctattt gagtgcattc actcaattga caagatttca
     8521 actgcaattg aggttgacaa agaagctaaa aaaattaata aaatacaaaa tatccttatt
     8581 gaagtaaata cgtcaggtga accatcaaaa tttggagttg aacctgaaaa agcggttgaa
     8641 ctttgcaagg ccatacgtga cctgcaaaac gttacctgca caggcctcat gacggtaggg
     8701 cctttgacag aggatcgcaa taaaatacgt gatgcattta aacaattgag acatttaaag
     8761 gaagaaataa accaaacatt aggcatttca ttacaggaac tatcgatggg aatgtcagga
     8821 gactttgata ttgccattga ggaaggtgcc acaatgctgc gtattggcac ggcaattttt
     8881 ggacagagga tatcgtgatg gatctgggaa atatatcaat tggttgtatt ggcctgggta
     8941 acatgggaag tgcaatatgc attggcctgt caaagttatt acattcagaa aacattattg
     9001 gctatgatgt cgatagttct aaagtaaaag aactttcaca aaaagttaat ctttcagctg
     9061 ccagttcaat tcaggaacta tcggacaaaa gcgatgttat tataattgcc gtaaaaccaa
     9121 acatggttga aacagtagcc cgtgaaatta aaagtaaaca aaaatctgca ctggttattt
     9181 caattgctgc aggtattact cttgccaggc tttctgaatg gctgggcgat agttaccgta
     9241 taatacgctg tatgcccaat acgcctgcgc tcattggcga aggcatgtca gtactatcaa
     9301 ccgtttctgc aaccgaaaat gacatacata ttgcgcagga tattttcagc gcattaggta
     9361 aagtaataat attgcccgaa caccttatgg atgctgttac tgcagtatca ggcagtggac
     9421 ctgcgtatgt ctttacattt atacaggcaa tggccgatgg aggtgttaaa gcaggacttt
     9481 cacgtaacga cgccctcacg ttagctgcgc aaactgtact gggtgcagca aaaatggttt
     9541 tgcagggcga ggagccaata attctgcggg gtaaagtaac ctcacctggt ggtaccacca
     9601 ttgatgctat tcatgtactg gaaaaaaacg gattttcagg tattgtcatg gatgctattg
     9661 aggctgcctg cagtaaatca aaaaaattgg gggaaaagta atgcttacaa actaccatat
     9721 atggcagata aaaaaagcaa tcaggttcat gataccagta atcattgcta tttttgttat
     9781 aggaaatatt atagctttct ttataaaaga tcccatcaaa gtgcctgata atgcgctgta
     9841 taaaaaaaat ggattacttc tttttaagga cgcatctgcg tatatatcac ttcttgaaaa
     9901 atatccatat aaccctggtg ttaaacttac aatttatact atccgtgaag gcgaatcgta
     9961 ctgggatgtt attcaacggt atggtatcag tattgatacc tttattggcg ccaaccctca
    10021 gttagattca ttacttgcaa aggctggtac aaaaattgtc atccctcaca aagatggcct
    10081 acttattgcc tgtaaacatt tctatgaggc atggcttgct aaactgtata caagtggtaa
    10141 aggttcaata agcggcagat acatacatca cccactggaa ttattagcaa tggatgatat
    10201 acgatttgca ttcattgaga atgcccgtcc tgtagcagta aacagtaaaa ttgaaaagtt
    10261 atatgcctta aggcaaaaat ttcaatcgcc cataaatgga aaatttacat ctatgtatgg
    10321 cgacagagtt gatccgcttg agcacggtat ggctttccat aatgggcttg atatctgtgc
    10381 accacagggt actccaataa aaccagtcca aagtggattt gtcaatttta ctggttggtt
    10441 ggatgggtat gggtttacaa tcattattca gcatgatgaa ggctatgaaa gctggtatgg
    10501 acattgccag aaaatatttg cccagacagg tgagtttgtg acaactgata ctatgattgg
    10561 aactgtaggt tcaacaggaa gatcaacagg tcctcatctt catttcatgc tactgcaaca
    10621 cggcaagatg cttaatccta tcatgtttat ctggtagctt ttttaagaaa aatttggatt
    10681 ttgaaataac cctttttctg cctttataag atcttgctca tcatccactt cataccaccc
    10741 aaatcctgaa aggtcacata ttgttggtgc agtccttgga ttttctgcca gtatttgtaa
    10801 aatattttca actaccgctt tttcaccaaa cctttctatt gcacggtatg ctgaatctct
    10861 gtaaactgtt tccatggttc tatcaacata cgtcatccct atatacccgc aatcaaaatc
    10921 atgcaattgt ttgcttatat gaattattgt cttcctatca acatctaact taaccttcat
    10981 atcatcagga cccagttgtc tgtcaaaatc acacatagca gtaattcgag taaaagattg
    11041 cttcattgtt tcaaacataa cctttggata aatatgatca acattggtta taaggaatga
    11101 atcatgggca aatgttttta atgcggtaat taatgtaaat atattgcctt tgtggtattc
    11161 cttattttca agtattttta aatgttctat ctgtttttgt ttcaaatgct tttcaagatc
    11221 attaaaggca aaaccaccta caacaatgat ttcatcaaaa aaattaagat caaaaaattc
    11281 aaataaatag tctataagag gctttccatt aacctgaacc atccctttgg ttttgttttt
    11341 agtcatttca cccaagcgac taccggtacc tgctgctaat acaattgcac gcatgataat
    11401 aactccttca atgattcaat tgttacaata tttggatatt tttgcaagaa ctgttctctg
    11461 gtaaatgtcc cacacaaacc tataaatttt atattatttg taatagcctt ttctgcatct
    11521 tttagtgaat cgccaacaaa agtcaaatca tctttttgca aattaaaatg ctgaataaca
    11581 tattcaaaat gctgcttgcc cttttcaaaa ccatctctaa aacccagtac tacatcaaat
    11641 tgcaaccctt ctctattgat aaattgctct atgagatatt gaaaattatt tgatgaaact
    11701 ccaactataa atcccaggcg tcgtaattca ttgatcacat agcgcacctc ttgcgaaaac
    11761 gttgatctga aaaagccttc tttttttgtt tcttcaaaga ttgcagcttt ctggctattg
    11821 gttggatttc ccggtaagat tatctccagt tgctgaaaaa aaggaacacc cgatgtttcc
    11881 aagtatcttc tacgtgcttc ctcaaacgaa atttccggat gaaatctatg tataacttca
    11941 ccggcaatat cagcaaatcc ttccatggta tctaccagag tgccatcaaa atcaaaaaga
    12001 agagcctttg tttgaatgat catgtacaat tattcctaat tttaataagc tctcagtaac
    12061 tatcgataaa atacaacttt tgttgcttat aatgtattga aaattaaaat tttctatcaa
    12121 tttcttgtaa cgcatatgat tgtcaataga aaaaattata tataagttac cataaatatc
    12181 ttgcctgtaa aagaaaatct acacaatact tgacatttat ttaaatatat ttacagtata
    12241 tgacatcatg acaaaaggaa ttatgaattc agatgaatac ataaaaatag aattatctga
    12301 aactattgat ctgcactgtt ttcatcccaa tgacataaaa ggtatacttg atgaattttt
    12361 agataatgcc tacacaaaag gctataaaaa ggttgagata atccatggga aaggaaaatc
    12421 agtaattaaa tcaattgtcc ataactatct caaaaacgat tcaagaatta ctgatttttt
    12481 tgataaacac ggcaattggg ggacaacaat tgtcattttg aaaattggag aataaaatga
    12541 atacaataat tatcaccgga ggcacaggat ttattggatc aaatctgaca caaaaactgc
    12601 tgacaaaggg atattcagtt gtaatattaa cccgtaaccc tgatagattt ttatcacaca
    12661 ccccagcatt gacatatgct ggatgggatg gaaaaactgc tgatggttgg ggtaatcttg
    12721 ttaatgaagc taaagctatt ataaatttgg ccggtgaatc aatcgcaagt accagatgga
    12781 ctaatgaaaa gaaaactaaa atattacaga gtcgaatcaa tgcaggcaat gccatcattg
    12841 atgcaattaa aaaagcaaaa cactatcctg aagtactgat acaggcttct gctattggct
    12901 actacggttc acgcagtgaa tcattgactg aaacatccag cccgggactg ggatttttgc
    12961 ctgatgttgc catacaatgg gaagcatcaa caaaagaagc cataaagtat ggcatacgcc
    13021 atgtcataat aaggacaggt ttggtgcttg gagaaaatga tggattttta aaaatattga
    13081 ttccggtatt caagtggtat ctgggtggtt atttcggtga tggctcacag tggatgtcct
    13141 ggattcatat tgaagatgaa gttaatgcca ttatacactg tttggaaaac gatgcaatca
    13201 aagggcctgt taatgctgtt agcccaaatc ctgttcaggc aaaatatttt tataaaacat
    13261 tagcaagtgt tttacgcagg ccatgtttat ttaaaatacc tgaatttgct attagattga
    13321 tactggggca aatggcggaa gaattgattt tatccagtca aaaagtccat cctgatgtgc
    13381 tgatatccac aaaatttaac tttacgtatg ctaatcttta tgaagcattg tattctttat
    13441 tatctaaaag atcacattaa tacacctgac tgaataaacc attctgcaac atgaataatt
    13501 taccggtgat tacatatgat gaagctcaga tgcttgaatg gcatgccctt ttatcatact
    13561 tcagggattt ttgctctact gaaactggaa aacagtattg tataaattta caacccctcc
    13621 agtaccagga tataattata cagtataata aaattaactg cttaatagaa attttacgtg
    13681 tagggcattc tcctgatttt tccggtatat atgatataac accgttactt gacaaagcag
    13741 caaaaggtgg aaccctttta ttagatgaaa tttttaaaat tcgcttgttc ctggttgcgc
    13801 aacacaggat tacatctttt tttagatcag tatactatga tatatcggtt cttacagaac
    13861 ttcatgcaat tgatgaatgt atacaacttt tcagactgtt gacaacttca ttaaccgaga
    13921 attgcaatct taatgaaaac acctaccctg ctttacgaaa aatacaaaaa gatattaacg
    13981 aaatgcgtag tataatagaa aagaatctta ctggtatcat gcattcacaa gcttatcagc
    14041 acgctttaca ggaaaggata ttcacagtaa aaaataatag atattgtatc ccggttaaat
    14101 catcacataa ggggaaaatg aaaggtactg ttttagatat ttcttcttca ggtgcaactt
    14161 tattcattga acctgaaagc attcatgaat tgaacaatac attgttatat aaagaagtag
    14221 aactgcagcg tgaaatagat aaaatactcc agattctctc ttccgaaatt ggtacctatg
    14281 cgcacgagtt aaaacataat gcaaccattt tagcctatct ggatttctgc gtagcctgtg
    14341 cacgttttgc cattacttac aatgcccata cacctgctgt ttcacctgct ccccttttag
    14401 actgcattaa tgccagacat ccactgcttg ctattctatt acgtgatgcc gtagtaccct
    14461 gcactatagc ttgtggtaaa tcattcaact gccttatcat atctggtgta aatactggtg
    14521 gcaaaacagt tctgcttaaa atgttaggat tgtttgctct tatggtacgt catggcttcc
    14581 caattacagc taaccctgat tcaaccattg ggtattttga acatatattt gttgatattg
    14641 gtgatgaaca gaatattatg caatcgctat ccactttcag cggtcagatt aaaaatattg
    14701 tacgaatact caatcacgcc aatgaaaaaa gtcttgtgtt gatagatgag ataattgttg
    14761 gtactgaccc aaaacagggt gcagctattg ctcaggcagt actggaacat cttgctgaca
    14821 aaaagtcaat catagcagtt accacacact actcacaact gaaggaaatt gcatcccatg
    14881 ataaccgttt tgaaaacgca tcagtagtat ttgatattca caccatgaaa ccaacatatc
    14941 aggttctttc aggtatacct ggtgcaagct acaccataga aatagctaag aacctatcac
    15001 tgcctgatac aattattaat aaggcactat cgctccttga tgcaacatct caatctattg
    15061 atgcccttct ttctaaaatc actgagcaat ctcacgctct gcagcaaaaa gaacaggaac
    15121 tatcgctcct gaaagaagaa ctacaagaat tgcagcaaga atacaataaa aaaattatgg
    15181 aacttcaaac aactattgaa aaagtcacca gggaacaggc cacttcattt atgaatgaac
    15241 tacaggaata tcgcaatatg attgtcaaca gaatcaagga aatacaatca atgtcaatga
    15301 aagatgcaac caccctgata caggatataa ctcaattgca agaaaaggtt aagagcacca
    15361 ttacatcatt gcacaagcct cacgaaaact ctgtgccatg caatactgaa actgcagaaa
    15421 aaggtgtgcg ggtatatata ccttcattac aacaatatgg tactattgaa gaagtagata
    15481 caaaagaaca tacagttaaa gttagaatgg gttcaattgc cagtagattc tcctttgatg
    15541 atgtgttctg tattgtagaa tcaaaaaaac ctgttgccat aaaaaagaaa acgcaagcta
    15601 taaaagaaga aataacctct atacctatta ccatacagac acagtataat accattgacc
    15661 tgcgaggttt acgtgttgat gaagcgttac aaaagttaga agctgaactt gatagtatgg
    15721 tgaaacgaaa tgtttcgtct gcagttgtta tccacggtca tggtacagga tcattgaaaa
    15781 aagcagtacg ggattatgta aaacattcat tctatgctaa aaatttccga cccggcgagc
    15841 ctgaagaagg cggtgacggc gttactatca ttcagttgcg atagcaggat tgcccataat
    15901 cacttgctgc aaataactgc ttcaaaagga tccagcgtga tggtttgcac atcaatgatg
    15961 tttttttgat gcgatgataa taacactctg catgtgctat caaaattgat ttttcttttt
    16021 gaagaagaaa aatttaatgc tacaagacat gtatcattgt tccacacacg tttatacagc
    16081 attacatcat cagatccggg taaaaattct atttcaccag catataattc tttacgagat
    16141 ttccttaatg caatcagttg ccggtaaaat tgcatcatag attctgcatt ttttgactgt
    16201 agtgctacat tagtgcgtgt atagtcagga tttactggca gccacggttt agcggttgaa
    16261 aagcctgcat agctggttgc atcccactgc atgggggtgc gcgctttatc tcttcctgta
    16321 tggaaaggcc agtatttttt gcctacagga tcctgaatat atctataagg gatcttccca
    16381 tcaaccattc caatttcttc gccataatat ataaaagggg tgcccttttg agtaagcagt
    16441 aaaaccgcaa gaagtttagc tttttgcaag tcattttttg ctatacgtgt aatagcacgc
    16501 gactgatcat gatttgacaa aacatggctg ggccatccct tgttgccaat agcattatac
    16561 cactgtataa gaatggatgt aaattctttt gcactaaacg atatatagat agtagaaaaa
    16621 tcaaatgaaa ggtgtaattc atcgttattc cctaaatacg aagcagccat cagtggttca
    16681 cgttcaccct cattgggata ggtttcacca acactcataa tctgaccata agaatctaac
    16741 aagtggcgta attcttttaa aatatcatgt atctcaggtt gatttctatc atagaggtga
    16801 tattgcaggt catgtcgtcg tggatatgtc aggcggaact tatacggatt gtttctgaaa
    16861 agtggatctt tcatgatata atttatcaca tcaaacctga aacctttgac tccattatcc
    16921 atccagaacc gcagtatatc aaacattgct tttttcacgt ttggattgcg ccagtttaga
    16981 tcaggttgtt gcttggtaaa aagatgaagg taatattctt gagttaatgg gtcccattcc
    17041 catgcccttc cgccaaaagc tgccatccag ttattgggca cttttctctt cttgcctgga
    17101 tgccatatat accagtctct tttaggatta tcttttgatg agcgtgattc taaaaaccat
    17161 ggatgcaaat gggatgtatg attaaaaacc atatccatga ttattgcaat atttcttttt
    17221 tctgcctctt taacaaggtg tttgaaatca cgcatggtac caaaaacagg gtcaatatcg
    17281 caataatcac tgacatcata tccaaaatca tacattggtg aacggtaaat tggcgatagc
    17341 cagatgccat caatacctaa atcctgcagg taatcaaggc actcagtaat tccgtttata
    17401 tcaccaatac catcatgatt ggaatcttta aagctgcggg gataaatttg atatattacc
    17461 ccgtgtttcc accagacttt cttcatgaat ttattgttta attattcgta gtctttttct
    17521 tttgattctt tgacttcctg tttggttaca gtcttttcca tctgagcttt tacatcatca
    17581 gcttttccca cagcctgtaa tgccttaaag tatgcagcag cggtttcata tactgcctgg
    17641 tcaatccttt cacgtatttt tgcatttgaa tggtattcct gtgccatagc atccttgaag
    17701 ggcttaaata actttttcat gttaccaccc ttcatgttat tggatagtgc atactcttcc
    17761 atctttttcc ataaatccat tgaaatgcct ttatcgggat ttttttgata tgaagggccc
    17821 ttttccactg caacaccata ttcatcagta tcaatacccc atattacatt ctgccaggtt
    17881 gtaccaacat tagctttgaa tattccatac ttataaaatt tcccaacatc ctctatagct
    17941 gttcccgttg tcccgtgctg cgcaattccc acaccccatg gtgccattgc cttataggta
    18001 ttataggtta gcttgagatc aatatttccc cgcacattgc catgcgtggt tccattatta
    18061 atggcaatta aatcaggaca ttgccctaac tttgccagtg cctcaacaag tgtaactgct
    18121 tcacttactg aagaattgcc atgagcacca ccaatctccc caacttcaga ctcatacatg
    18181 agtcctgccc gtacaacctg ctcacttaaa agagcactca atgcaatatt ctgttccatt
    18241 ggcaaaaaag aacaatctat tgaaaaggaa ccatatcccg cctcaataga agcttttata
    18301 agggaatcag ctttatcaaa cgctttacgg gttatttcct gtacagtggt gtgatctcta
    18361 tggcaggtga atggaacagc tttttcacca acatttccca ttttttcatt taatgttact
    18421 accagtgtaa taaaatcttt aggtgtcaat ccggtatatc caccggttaa atcactttcg
    18481 gataatgcca gttcataaat caccggtgcc tgtaattcca tggaagcttt tatcaatccg
    18541 ggaatagcaa tttcatgccg tgtattaaca gccatcacca cccagtggtt tccttctttc
    18601 attgcagatt gtataattga ttttccgttt acatgcatca ctttggaatc agggaatgct
    18661 tttttaaaat tttcaggacg taaatccaga atactcatat attcctccgt tattatttat
    18721 ataattgatg tatgaaaacc tatcattatc aaaaatttaa tcaagcataa tttttaacac
    18781 taaaaaatat tctcacctat cagcatttac tatctgtctt ataattctgt ttccattgtt
    18841 atacagtatg ttttccaatg ttgttttgct taatccgtat ttcattaatg tctcgatctc
    18901 caactcacgg ggaaagatta tattgggaaa atcagaacca tacaaaattc tatcctgata
    18961 tttttccagc acttccggat ctttatggaa agcaccttca taatctggta aaaacacaaa
    19021 cgctgtatcc atcataagat ggggataatc atctaaaagt tctaaaaatt catcatattc
    19081 atacgcaccc atatgggcga tagttaccgg tatatcagga tattgcctca gtaatttctt
    19141 aaatccgcca atcccaacaa attcgtttcc caccggaccg gtgccaacat gcatcaggat
    19201 gcgcttttta tagtgttgta ccagttcata cagtgggaaa aggcgtgtat cgtaaggata
    19261 acatttctga actaaaagct gtaacttaaa ccccagtacc tttggatggg taataaaatc
    19321 ttcagcaatt gcgatatcat catcaggatg atatgcgcca aagcaataca cattgtcctg
    19381 ttcatccaga accttcaggt tccaatcatt taaatgtttt gcaacacctt ctttatgagc
    19441 ataatttgaa tacacaattt tttcaatacc acgttcttta agatatgcaa tacattcctt
    19501 ataatacagg tgatacataa catcccaacc atagtctttc acaaaagatt tccagatagc
    19561 atcaaacatg cggtcaggga aaagatgtac atgaaaatca atgattggat aaggatatgt
    19621 ggtcattttt taccatattc tttgcgatat tgttttggtg acatcccggt catttttgaa
    19681 aaaaccgtac agaatgtggt attggaatta aaccccacag caacaccaat agataatata
    19741 tctagccctt taatccgtga acgttcatat cgtgccttca tagcctcact tnnnnnnnnn
    19801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntatatctag ccctttaatc cgtgaacgtt
    19921 catatcgtgc cttcatagcc tcacttttta aaagcttgtt ataatcagga tgacgctgtg
    19981 taacaaggta cacaaaagtc attgctgtat tggctatgat cactgcccat tccactacgt
    20041 gtaatgaaac aaatgcccca tatacacata ttgcattgga taccactatg gataacgcaa
    20101 agatgtagcc catacgtgaa acgccaggca cagtattcca ttttttccta ttccatacgt
    20161 atgccatagt tatcaaatta ggcaacataa acgtaataag atagatattg ggaattataa
    20221 aaagcaggta cacaccttta tcataaagcg atagttcata gaatctgtca cttagcagtg
    20281 gtaatccagg gaaacatgct gaaccgcatg aaatgccctt gataaacaaa ggtaccagca
    20341 aatagaataa agaaattatg gatggcaaca atacaatata atagattttc tttacatcaa
    20401 agaggcttgt tattatccag gtgtatcgca tcaccataag tgggggaaca ataaaaatta
    20461 cgggaattga agcaatactt acatagtacc caatagcgga actatcgaac agacctgacg
    20521 aataaatgca tatttgcaaa agccaaattc ctaagccaaa gaaagaaact gcatatagtt
    20581 tattaatggt tacaattttc ttctgcacaa actgccctag ggcaataatc actgaaaaaa
    20641 ctgcaccata gaacataaaa aaatgtaata ctgattcact gctcataaat actcaaattt
    20701 atataattat aattttaaaa ttattaaact ttcaaagtta taactctgaa agacttttaa
    20761 acatataaat gatatactct gaatagcatt atacaatacc atccggtcgt tatctggctt
    20821 taccggatgt aacctatcat gggaggttat attatgactc agcatcaaaa tgtcaattat
    20881 gaaaaggaat ttgaagaata ccttaatagg gaaaagcacc ttgctctgat ggtgcttaac
    20941 agggctaaaa aatttggtga ttcaaaaatt gctgtacgcc acaaagccta tggtacatgg
    21001 gaagcattta cctggaagca atttggtgat ctaattgaat cggcagccag aggattgctc
    21061 ctttttggag taaaggaatt tgaatttgtt ggtatttttt caaataacag agtggaatgg
    21121 gctgttgttg attatgcttc atttgcaata agggcatgct ctgtaccggt ttatgccaca
    21181 aactcagcca aagaacttga atatatagtc aacgattgcg gaatacgcat tctttttgtt
    21241 gaaaagcagg aacactatga caaagcaatg agtataattg attcagcaaa aagccttgaa
    21301 aaaatcattg tttttgaccg taatacaaaa attgccagtg acccacgtgt tatgctgttt
    21361 gatgacttta ttgctatggg caaaagtaag agcatttatg cacaattaca agaacgctta
    21421 tcaaaagtgc aaagtgatga cctttcaaca cttatttaca cttcaggtac aacaggtgaa
    21481 ccaaaaggtg ttatgcttac ccataaaaac tggtttgcca tgctgtttgg aacggggtat
    21541 cacattccgg tagaaccaac agatgttaat ttagcatttt tgccgttatc acatgttttt
    21601 gaacgggcat ggagttattt cattctttgc aatgatgcac aggtagatta ctgccatgat
    21661 acaaaagcac ttcttgactt cttaaaagaa tccagaccac actttatgtg cagtgttcca
    21721 cgaatgtggg aaaaggtata tgccacaatt atggatggca taaaaaatgc acctcctgca
    21781 aaacagaacc tattcaactg ggcactggaa atcggtggga aataccagta tctaaagaag
    21841 gaccacaaac ctattccaat aggattaaaa cttaaacatg cattggctac aaaactggtg
    21901 ttacataaaa ttcgtgacct ctttggtggc aggaacaagg tattcaattg tggtggttct
    21961 gccttttcag gtgaaattag cgaattcttc ttcaaagcag gtgtatttat tttacagggt
    22021 tatggcatga ctgaatgttt tgtaatttgt gtatccaacc ccgaaagaaa taaatttggc
    22081 acctgtggtc ctgttgttcc acttatgcag gtgcgtgtat cgcctgaagg tgaaattcag
    22141 gccaaaggtc ccagcatgac ccagggatac tggaataaac ctgaacttac cagggctctc
    22201 tttactgaag atggatggat taaaactggt gatgttggct ttattgacag tgaaggtttc
    22261 attacaataa ctgaccgaat aaaagacctg ttcaaaacct ctggcggaaa atatattgca
    22321 ccacagcaaa ttgaaatgtt attcaaagaa gatatatact ttgaacagac tgcagcaatt
    22381 ggggatggcc gtaaatttgt ttcagcatta attgttccgg catttgaaca actggaaaaa
    22441 tgggcaaaaa acaataatat tagcttttct tcacgggaag acctggtaaa acatcctgaa
    22501 gttattaaat tttaccgtga aagaatagac tatttaacac gtgatttagg tcaggttgaa
    22561 aagataaaaa aattcacctt acttccccat gaatttactc aggaaagtgg tgaaatgaca
    22621 cccactcaga aaatacgacg caaagtaatc aatgaacatt ataaagatat tattgaagca
    22681 atgtatgctg agtaagtaaa aatccgccgc tatctttttt ggcggcggat tttttcttta
    22741 aaaaaataca tgaccacttg acattaacta tctttttact tatatttaag aaaatactta
    22801 aaatctaaaa aggagtcaac ttatgtacaa tagtaaaaca attataacaa taagttttat
    22861 ttttattgcc actatggcac ttgcacagca tcaacacggc aaaggtatga tgtatcatat
    22921 gtatgatcca tcatcagaag taaccgtttc aggtacagtt gaagatacag taacagaagt
    22981 ccacccatgt tgtggttcaa ctatgaacca ttattatatt aaagtaaaaa ctaataatga
    23041 aacagttaaa atatttttag ggccaactga ctatgttaaa tcacagataa ctattaaaaa
    23101 aggtgacaat gtaacaatcc tgggttcaaa aacaacaatg aataatgaaa ctgttatcat
    23161 tacaaaaact gtcaaaacca aagataaaga agtaactttc cgtaatgatg acggtacacc
    23221 aaaatgggcc ggtcagatga agtataaaaa gtataaaatg aatcaataat tattttgttt
    23281 ttaacctgta tacaccatcc cattttttcg gtggtggatt tttagcaaat tccttgcaac
    23341 gttctatata cgttttacag gggccatcat caggaacaat gcgcaatccc gctttaaaag
    23401 cagattgcgc ttcttcccat tgtctttctc tgaatagctg taatccttca ttatatttct
    23461 tcaacatttc atacatataa tctggaagcg tgcctttttt tccaaataat tcatatacct
    23521 taattggctg ctctttccca acaacccgta ttaaatctaa ttcccttgct tcaacaacat
    23581 cttttacccg ttcataggtt gtttcactga tcatagtata tgtaccataa tacttgttag
    23641 ctccctccaa tcgcgaagcc aggttaacgg catcacccat catggtataa tccatacgct
    23701 gccgggatcc catattacca acaacagcct cacctgtatt aattcccatt cttactttca
    23761 gctcaaatgt gcctttttta cgccattctt ccctgagttc agccaaccgt cttttcatat
    23821 caatagctgc caggcacacc ctttcaggat gatcctcgaa tgcaagtggt gcaccaaaaa
    23881 aggctattat tgcatcccct tcatatttat ctaccgtacc ttcatatttt aagattatat
    23941 ctgtcatttc tgataagtat tcattgagta atgcaacaag ttcctggggt gttaattttt
    24001 cagaaatgga tgaaaatcca gccacatctg agaaaaagat acttatattt cgcagttctc
    24061 ctcctaactg taatttacct ggatccttta tcagttcttc aactacacgt ggtgccatat
    24121 atttagaaaa cacttcttta atataccgtt tctggctttc ctcactcata aacttgacac
    24181 tacttactat tgctgatggc aacagtatag ccagcgacac acccagttga tccatataaa
    24241 gattttgaaa tgcgaataat gctatgttta taatattgta aacaacaagc gttcccacaa
    24301 ttgctgtaat tgtacccatt gcacttaacc gttgaacagc aagtaccaaa aatattgcca
    24361 gcaataaaat taaacaataa tctatccatt gaggaacctg ataaatgtag gcttgctgta
    24421 aaaccgtgtt aataaaatta tggtatgtac caaccattag atattctgat gataggggtg
    24481 tcaccccaat atcatgtgaa gctgttgctg ttaatcctat tatgcatgaa tgatctttta
    24541 atctgtcgac ctcaagcacc aggcgtgcag cgttaataaa attaattaaa tttttactgg
    24601 cctctttcaa ttgattatcg ttggtttctt ttaattgctt atttatttca gctaactgat
    24661 tttccagatt ctttatatat ccactttcta tctcatacaa ccgtttacgg ttagttttta
    24721 ttttttccca tagatcctgt ttggctttca tgtcttttgt tttttcaaat atttcatgca
    24781 tggtagccag ttcaccatat aacctgcttc gttcggtgtt tttggactgc acttcctgtt
    24841 catcaaagaa ttcatgtatt gtgtctttta attgattata ttcaagcagt gcataaaatg
    24901 atacatgatt aaaggtttca gtaaattcgc cactccagtg aatataggac ataccagcat
    24961 catcaatagg aatgacgtaa tccttttttt gcaatgttat tgggtcaatt gcattgtgca
    25021 gtatgatatg ttttccatga actatctcca catcagtttt tttaactttc attaaatcca
    25081 tcagcataac catagccata tggaaatata ttctatcatt gaatacacgt ataagtcgta
    25141 tgcggcgggc aataccatca atatcaaagt cactatccac atatccaaaa tccctggcct
    25201 tcatggcaag ttctggaatt ggatattgaa taattactct ggttgggtca acataacgag
    25261 aaaattcctt ttgcttatct ttgggaaccg gaattgaagc tattttctca aatctctgga
    25321 tggattcgtt ccgtttttgc tgctcttcgg gtgttaattt tgtttctgca agagtgcgat
    25381 ttaaaaacga atacgccaga ataacattat ttttggcgat agcttctgaa aatagtcggt
    25441 cgctgtccag tattgcctga ttaagttctt cgtaagtgat tcgcttgcca gtttttactt
    25501 tttcttctac aattttcaat atatcaggtt gagcataccg aggtgattta tccataaatt
    25561 gaatatcaaa tgctatctgt tttggcgcta tatcctttat gacttcaaga ccattagcgt
    25621 agatataccg cggccatgga aattgaccta cattgctgat gctgttatca tcaatatcaa
    25681 gaaaggttag atattcccat tgtggaagct gtggttttat tttgaaccgc acatcaaata
    25741 atttatactc aaaaagcctg tatacattag tgaataaaaa accaataata agcaaaccca
    25801 gaatcaagcc tatagcagcc ccatataacg gatttgattc aatatttttt cttatagtat
    25861 caaactttat ttctggcata aaccacctgc ttatctttat ttttctgcat tataacagat
    25921 tatgtttaaa tattacaagt ataataatat tttgttatct tatttattcc atttcttttg
    25981 gatacaaaga tatctttaat tatcctgcca caaaaagtgg taaaggtaat aaattttata
    26041 aatttacaaa atagtatttt ttgaaattat cagacaaata ctaaattatt ttatgcagtt
    26101 ttattgtatt ctgattgata gtgcatcaaa cgtattttca agattacata ttccatagct
    26161 tttataaaag cctcattaat gggatatcgt tcacctctgg aaggatctat catcattgac
    26221 tgcatagaaa gagaactcat atgcggtaaa ttactgaaat ggatggcagc tatttctttt
    26281 tttgcagtat actgattttg cagcgtgcta catgctacag gtactatcgc cccatattta
    26341 tttttcataa aactaaaacc aaataagcga cacaccaatg gcctgtattc atacacagca
    26401 caacaccctg catcatcgta taatgtttca gctttataga aagggcacag gttgtctgaa
    26461 gccttatcaa gccataactg gtaattagtg tgttcataca aatatacaca taatggtaac
    26521 atttcaatta ttgacgcttc aatattgtgg ctggctgttt tgcagcactg accacagccg
    26581 tgaggacagc tgacagaaaa caaatttttt acatttttgg tgattttttc aacttcctca
    26641 tacaactgga taattttttg tagttgtgga aataacgaat aataattttt actaatatac
    26701 ataatcacac aacaccatta tcgtaaatta gaaaattatt attgttacat caggtagaaa
    26761 attcaaaata tgaaacgtac tgtcaacttt tttatttatt taatgcatca aaagattctt
    26821 taaatgcttg tactcgttta gaccataaag aatatttttt tctttcaagc tttgagtata
    26881 tgcatccaca atatttctgg cgatacatct cgtattgttt tgataattct attgaatgct
    26941 gataaccacc atctaattta aaatcagaag gtatatatgt tatgttatat acttttgaca
    27001 gcgttttgcc tatctcattt attttttcag catctttata tgggctgaca gttaacgtgg
    27061 tgcatacagc atcaaaattt tgttcgcgcg cacgtttaaa tgtttcctct aaacggaatg
    27121 catagcatgc atgacagcgc ggcgatagct cctcataaaa gcggtaaggc tttacggcat
    27181 taaaccattc tctttgatta tagtgtccga tagttaccgg aattgaatga agatgtgaaa
    27241 attgttccac ttcatacaat cgccttttat attctgacag tggagatata ttgggattat
    27301 aataaaaaat gtggatatta tattgagaca caagaacaga gtgtacatgt gttaaacacg
    27361 gtccacaaca tgtgtgtaaa agtacagttt gtaattgact aactttcact taagatattt
    27421 atcgataaaa ctatttaact ttttcaactt tgcaggattt actttgccat caatccacaa
    27481 taaaaggtct gcattcacac cacgggtaca attgtattcg tgatgaatca caaaatttgc
    27541 aatttcaaat ttcagctttt caaaaatctc ttcagctata cacagatttg aaggctcagg
    27601 tccaatacag tcagcaattt ttacgatgat ttcatgtttg ttgatttctt tgatttcctc
    27661 acggtaaggt tcgtcaaaaa tacatttagc taaacaaata ttgtctgaag gagataaata
    27721 ccatccattt accccaaaag aagccttgtt tcttttcttc attgcttatt cctcttgtac
    27781 catggcaaga tataaaaaat atgtaaaagc aatataatat ttctattaaa tatataaaaa
    27841 taataacaaa atgtcaaata tttaaaacta aattgtttca tgaaatgaat ttttttttat
    27901 attttatata catttgccat ttgtgattaa aattatttaa tttttttact tgacatgatt
    27961 acattaaatt ggttattgat ttttacatct ttgaattaat gtactattta tgaaactatc
    28021 attgtataac atcatcagca aaagctatta tttctggttt tattattttt atagcagggc
    28081 tcgtctgtag cttttgctga tgctgttatt atgcaagggc cgcaggcggg tagccggcgg
    28141 cctttgttat ttatggaata atgtataagg ccgccaccct ccgggctctt aattgacttt
    28201 aaaatttttc ggaggaatat atggccacat gtaatgcaaa tcctgtttca ttatcctgca
    28261 atattactaa tgttgcaaca ccatcatcac cgctttatag taaaacttat cgtgatgctt
    28321 cggtaattca cctgccctat aatatatcaa tttctgaaaa cacattggca gtcatagcag
    28381 gcccatgttc aattgaatct gaagaacaga ttatggcaac cgctgaattt gtaaagaaat
    28441 caggtgcagc tatattacgt ggcggtgctt tcaaaccaag gacatctcca tatgcattcc
    28501 agggtatggg tattgcagga ttacagttgt tacgaaaagc aggtgactat tacagaatgc
    28561 ctattattac ggaagttctt gatagtgaag atattactat tgtagctgac tatgctgata
    28621 tcctccaggt tggcgcccgc aattgccaga atttttccct gctgagaaaa ttgggaaaaa
    28681 ttcgtaaacc agtactttta aaacgtggaa tgatggttac tataaaagaa tttttaatgg
    28741 ctgcagaata tatattagct gaagggaatg aaaatgtcat attgtgcgaa aggggtatcc
    28801 gaacatttga aacagaaact cggaatacgc ttgacatatc tgcagtgcca gttttgaagc
    28861 aaaaaacaca cctgccagtt attgttgatc cttcacatgc agctggaaac tggaagctca
    28921 tccatccttt agcacttgca gcattagctg caggcgctga tggcctgatg attgaatgcc
    28981 atataaaacc tgaatcagca ctgtgtgatg gtgaacaatc attaaggccg gataaatttg
    29041 cccttttgat gaatgatata cgtaaaatga ataccttgct taataaaaaa ctggaatgta
    29101 tctaaacaat aataaatggt acccgaatgc caacggaaac tattaatgaa ccatcacaac
    29161 gatacaggtc gccatccata gtatacataa acggttttgt tgaatggatt tcaattgtac
    29221 taacaacttt ttcaatataa tctttatggt ttataggtgt gccatttttt agttttagta
    29281 cattctttgc cagtttccct ggtttcattg cacatactat agtatgaaat ttatctttat
    29341 gttcatttgc tctgtataag ggataaaagc ccatacctat agtttctacc gttcctgcaa
    29401 gtatacccaa aacacgtgaa tatggcatag attccgcatc acatttaata tttccttcaa
    29461 aacctttaaa taaatgtgat tgctgattag ccaatgcttc ataaatactg ctaaatatta
    29521 agcctaccag tttagtataa cttttaccaa tactatagta agtttccaga aaatctgcaa
    29581 caaggccatt cccaaataaa aaacagtgat tattatctat aaccattgtt ttgcgatcta
    29641 caacgttaat atatttccca ttgtttatgg caataacagc cctctgcaat attgattccg
    29701 catcaccttt taaattaata ctgttggcaa cattgttcat tgtaccacct tttaatatta
    29761 aaataggagg caggttattg ttatacactt ttgcaaactg cgtaataaca tgatgcaatg
    29821 tgccatcacc acctgaagga gcaatataat caatacctgt ctttttaaac tcgcgcgcaa
    29881 catcaactat ttcttctatt gattttgtat atttcactat gacactatcc tgcccaattt
    29941 tttcaaaaac accttctgga tgtttctttg ctaatttata ttttctggag ttgagattaa
    30001 caataacacc aacttttttc atacagtatc cttttattat ggtatgtaat ttcagatcaa
    30061 gtttacattt tctatatgtg tatggtgtat gtgtcaaacc gtttttggtt accattaaaa
    30121 atatattgac ctgatatgta tagaaaatat acatattaca tataacaaaa tgaaacataa
    30181 ctaagtatat ctggattata atcttttatg gcaattattt gtatacgtgg tggatctcta
    30241 tcagcggggc ataatgttaa aacatcatat gttgatttgc tatcacatga tggactactt
    30301 caaactcata cattaattaa tatttctaaa ttgggcgata gttcctttga aggagtatgg
    30361 cagtttgata ccgtactatt gcataaacca gatattttaa tacttcattt tggaatagac
    30421 gatatttaca ggccagtgta ccgttcagaa tttaaagaaa accttgtgcg catggtacag
    30481 aaagcacgca atgcatcaat taataacatc atcctgacaa cactgcatct agttagaaat
    30541 caatacgata tggatgctgt ggacgttttt acgcgaaccg tacgtgaagt tgccttagat
    30601 atgaattgtc tattagcaac agttcatata gaatggataa actatctttt tgaaacagga
    30661 aaaagtctta atactttatt aaccgctgat gacagatatc ccaatgaaaa aggacatgca
    30721 ttaattgctg aagctatcaa gaagaagctg gttcaattac tttcataaag taataaaaag
    30781 gcacatggtt ttgttcatag attgccatgt gccttttaca aaatttattt aatatttcca
    30841 gtaacctttt ttatttgctc ttcagcttct ttgttcatat tttcaagcag ttctgcacag
    30901 gttggaatat catctataag gccaatggat tgtccaacca ttaatggtgc tgcattgaca
    30961 tcaccctgct cccatgcctt gctcattcgt tcacctgata ttaatgggaa caattcttca
    31021 aagcctccac ctcgctgttc aatttcaagt attttattta caacttcatt ctttaaagcc
    31081 cttccttgta aatgcaggct tttcataata agtgtggttt catattcctg gcgtttgaca
    31141 agctcctgct ttatattatc atgcacttca cattcttttg tagctataaa acggcttgcc
    31201 atcattacgc cttcagcacc taacatcaac gctgctgcca tagttttacc atttgcaata
    31261 ccgccaactg taataactgg tattttaacc gtttgtgcaa tttttgtagt tagcaccatt
    31321 gtagttacat catcactcaa cggatgccca ccttcttcta tccctgctgc atatattcca
    31381 tcatatccaa cgtgttcagc atgaacggca tgacgtactg cacccacttt atgaaaaagt
    31441 ttgacacctg cctttttaag catgtcaata tattccatgc cacaggcttt gtcaataggg
    31501 gcgcctgaaa cttcaatccc tgcaactttt tcttcggcac acactttaag atacattttg
    31561 tgatgctctc ctgtgatgcg aacagacggc aatattgtga cattgacaaa aaaaggcctg
    31621 tcagttagtt ttcgtgtttc acggatagca tttctgaatt cttcttcggt attataatta
    31681 ccagccgtca gatttcctat cccacccgca tttgaaatag cagcgcacaa ttttggttta
    31741 catagccaca tcattgcacc acaaataatg ggatatttta ccccaaacat ctcagtaatc
    31801 ttagttttaa tcatatagaa actctcctga aaaaaatatt tatttaaatt attatttcac
    31861 aatcacttat gggaaatgct gcacatgaat ggatataccc aaaacgccta tctgcctgtc
    31921 tcagtttcac tccttcaggt tgatacactt taccactcac taactttact ctgcataaac
    31981 tgcattcacc gctcctgcac agtgatggta ccacaatccc gtttttttca agtgattgaa
    32041 gtattggttc accggcatta acagtaatat cttttttgcc gcgtatctta ataacaaatg
    32101 ttgaatttct gctaatatct tttggccacc caggctgttc aaaaacatta gagggaacac
    32161 cgtacatctc cattcgaagc tgccttggtt ttaatgtaag gggctttagt tctttcaaca
    32221 ggtgttcata tagtgcttgt ggtccgcaga caaagaatgt tttatcggaa ctatcgccca
    32281 ctacttcttt tatgattttt gctgtaatat accctttata ggtgcccgtg ccttcttcaa
    32341 gtacagggat atagtgaaaa aatttatatt tggacgctac ctttgcaaag taatcatcaa
    32401 aaatcagttc atctgcattt ttacttccat agataagcca tatttccctt tcagttcccg
    32461 tattgaccgc atgctgaatc atggacttaa atggtgtcac gccgctacca cctgcaatac
    32521 atacaagctt tgttccatga atgattggat tgtgataaaa atttcctgca ggacctgata
    32581 tctgtaaact atcgccaatt gtaaggctat caagtagatg attagaaacc aaaccgcctt
    32641 caacacgacg taccgttata tcataatatg caatttcatc aggggaagac gatatactgt
    32701 acggacgtga tgtgcgtatt ccgtttattt caacaatcac tgatatatat tgtccagcct
    32761 gaaatggtgg cagatacttt tgctttgatg ccagccgtaa tgttttggtg gattttgttt
    32821 cttctataat atcaattact tttacatcaa ttatctgtgg atgcaaccgt tcaacatatt
    32881 ttttaaccag tccccgttcc agtctgtaat catacccata acgtttgagg acatctatgt
    32941 cagttataac tctgtcatac ccttcaagtt gttttaatat ctggctgtcc atgtgttccc
    33001 tcctatttgt ttcgcatttc tttcaggata gctcttgctg ctgaatgccc tgaagtaagt
    33061 gttggctgat aaccagctga accataccag ctgcctgcac aatacagccc atcaataggc
    33121 ggttttggcg aaacaaacaa cgtagagtct tttacatact ggtcaaaacc gtatgctcca
    33181 ccacctggat ggcccaaata acgcatatgt gtaattggtg tagcaatttc catttcctca
    33241 atatggctaa taaggcctgg aaaaactttt tctgcatttt ttaacatagc ttcagcacaa
    33301 cggtattttt catcattgta ctgatgcggg ggcactttca gccatggttc agcatacttc
    33361 atggtgacca gtacaacttg cgatgtgcct tttggcacca tttcattatc aaaaacatta
    33421 tagcaggtta aaatgaatga acttttatca gtttcaagtg ttttcgatag ttcataatca
    33481 aattccatat ctgtggtatt gctgataaag tttgtttctt cattaatgcc gacttcgtga
    33541 tattcacaat ccagtccgca atatatggta aaaaaagatg ttcctacgct gctcccctta
    33601 tattgctcca gaactttgtg atttgccgca tcatccatca tgtctacaaa ggtgcttatt
    33661 gctgaagcat ttgacagcac atacgttgat tcaaattcct gccctgatga agtgcgaacg
    33721 ccataaactt tattattttt taccagtagt ttttcaactt cagtgttaaa taaaacctct
    33781 cctccatttt catataccac ttcaagcatt gcatttgaca gcatctgcga cccgcctttt
    33841 atatgatatg gcttaaactc tacataagaa aacagcaatg ctgccatatc actgaaacaa
    33901 agctttgaag gtggtagtcc catatatgtc cagtatgggc ttatgaccgc tttcagtatc
    33961 ggatcagtaa aatattcgtc aagtactgat tgagtatctt ttaaagcata tttataatat
    34021 aagggatatt tcttaggatt aatatctgga tcatttaagt aaaaagctcc tataacttca
    34081 gtaaaaaact gccatacaaa atcaaaaaac ttctgtattg catctttttc ttttgggaac
    34141 ttgccctgca acactttaat cagttcctct ttatttgtcg gcagcgtaac atctattaca
    34201 ccaggcaaag ttacccggta aagtttttcc atttcaacaa attccagttt attcagcaca
    34261 ccaatgctat ccagtacact tcttaagggt cctggtcgtt caggagtacc caacccgctt
    34321 aactggtgaa gcgagacttc aaactcaaat cttcctctgc aaaagcttgt tgcacaccca
    34381 cccggaatat tatgcttttc tattaaaagg gttttaactc cttttcgggc aagagtggca
    34441 gctgcagata aacctgcatt tcctgcacca ataattatta catcatacat tacagaacct
    34501 cctataacat aacattgtta tgttaaaata cattctattt tagtcaacta tttttaaata
    34561 aaaaataaaa gaggggttca tagtccccct cttattgaat ttctataata atgatcaatt
    34621 attatatgtt tatcgttttt caagtggtat atattcctta aattcatcca gagaagaggc
    34681 ataggcaggc cgtatgatct tatcgtgtat aatctgttca atcctgtgag ccgaccaacc
    34741 gctaaaccgt gccatggcaa aaataggtgt aaacaattcc ataggaatac ccatcatctt
    34801 ataaatcaat cctgaataaa aatcaacatt tggtgaagca actgttcctt tatatttctg
    34861 tactaaacgc gaaccaatat ctgcaacttt tgcaaaaagg ttaaattcat ctaaattctt
    34921 tttcattttt gcaaattctt cagccttttg tctgaaaata atagcacgtg gatcggattt
    34981 tgtatagact gcatgcccta taccatatat ttttccactt ctgtcataag ctttcttttc
    35041 cagcataagc ttaagataat gttccacttc ttcatcatcg ttccaatctt taacatgctg
    35101 ctttatatct ttcatcattt tcatcacagc ttcgtttgct ccaccgtgca aatgaccact
    35161 taatgaggcc acaccagcac ataaagccat atacgtatta gcaccactgg atgtaacagt
    35221 ccttaccgca aaggtggaat tatttccacc accatgctct gcatgcaaca taagagctaa
    35281 atcaaaaagt ttagcttcat attcatcagg tctttcacca cgtaacaaat ataaaaagtt
    35341 ttcagcagta ctccatgcag ggtttggttt tgtaataaat aaatcgccac cttttctgaa
    35401 tctcatgaca ttgtaattat atgcaatgat tacaggtgct tttgcaataa tatttataca
    35461 ctgtctgctt acatctttaa tatcagttga atctggatct ggatcacacc tgcttaggtg
    35521 tgatattaca gaatgcaatg catacatctg attatagtta acgtcttcct gcatgatgat
    35581 acttcgttcc agcttagtga gcggacgtct atcagctatt acctcttcaa atcgctgtag
    35641 atctttttta ttgggcaatt ccccagttaa aagtaaatat gttatttcat taaatccgta
    35701 cctaccttct tcttgaatac gctgtgccaa ttcacaggcg tcatagccac aatagtacag
    35761 cttcccctca accggcttta cagtatacgt tccatcagga tttggaattt tttcataccc
    35821 aattactata ccttttgaag taataccagc aacaacacct gaaccatcag gattccgaag
    35881 tcctaatttg atatctttct ttttgatcaa ttcaggtgaa atggcatcat gttcaagcat
    35941 tagtttttca atctttttta aaatttgttc catccactcc tccgtataat ttgatatgta
    36001 cgtttccatt gtctttgcaa gcatcatagt cattttaatt atccttttat cgattattga
    36061 ctatgatact cagtatacta tattgtacaa aaacatctac tttaaatatt taaattttcc
    36121 gtttatttaa cattattgta aaaaaagaat tgaagttttg agggattttc aaagcatcaa
    36181 aaaaggttac ctatatagta tcagcttttt ataactattt ttttgtcaag cttaaaattt
    36241 acttaatcct acacaaaact gattcatggg gtttcaattc acccaaatcc aaatggtcac
    36301 tatatacata aatttctctg ttaccccaca catcactcag tttacttgcc gatgttaaaa
    36361 tcttctttaa caatccttta agttggacta tctttttcct tgagctatcg gaaaaattaa
    36421 aaaatgcaat ataaccagcc gaattataaa taatttcagg tatatcattt tccataatat
    36481 caactggata tgatttctct aaactgcata tcctaatcag ttcatctatg gtatctataa
    36541 gtttccaatc atcattgtgt aataaagcta aattatctga aataaaatac atgccacccg
    36601 ttataataac cgcattgatc agcgtcattc tttcttcttt tgaaagtttt gtttctgtct
    36661 gtcgcaacat aatacaatct ggatcattta gccataaata tctgtgcatc tgagatcggt
    36721 taagtatact tcgtattgca aatttggtac acaacgcatg tggatcgcgt gtaagatagt
    36781 aacggtattt agccatccag tagggagcaa catcagggcc aatacgcata ccatcaacat
    36841 gaccaattga tgctgataaa ggagcaccac atcctaagat aaaaactctt tcaccggcaa
    36901 catccctaat aagagaatat cccaatttca gacgctgagc tgaagttaaa ctcatatcgt
    36961 atgcccttcc gcataatgat gcagcataca caaaatccag ttttaaatat ccaaatcccc
    37021 attcatggac aaacgtatta ataacttctt taatgtagtc ttgaaactca ggatgtgtgg
    37081 tatccagagc ataaaaatat ccaaatatat cccaattggg attccaacct gcaataactg
    37141 gttttccatc actatcatgc agaaaccagt caggatgttg ctgataaagc attgattttc
    37201 gtgaagcaat aaaaggagca agccatatac ctgggaccat ccccatagtc ctgatcttgc
    37261 gagcaattgg agccagtccg ccgggaaatt tattatttat agataaccag tcacctattt
    37321 cagtttgata accatcatct atctgaaaaa catcaaaccg ttttccttta cttctcaaaa
    37381 catcaagatt atgataaatg cttttttcat taatttttgt ataataatag taccaggaac
    37441 accacccctt aggtattttt cttcgtattg gtatctgaga acgaatgagt tcaaaatatc
    37501 gatcaaaagc ttcattaata tttttattgg taataaagta aattaaatca gcccttaatt
    37561 ttaatgcagg actgactatt actttacaga aatcatgaac tatatcaagc tgtattgtgt
    37621 tcccggtagt taaggtattt atataaacat attgattaaa cggccttccc tgacctatca
    37681 cacagtatga tgaattttca gaaccaatag ctataaacat ggtactatta aaagatccgt
    37741 ttttaccaga aggtaaattt ctaatagtat cctgtaagat attcatacag gtaagccaac
    37801 tcaccatttc tttttcatca aatgaataag acctagttaa gctccaggac tgaaagccat
    37861 ttttaaatac ataatacttc tcagttttag ggatagagaa tcttccaatt gatgtaataa
    37921 ttttatctaa ggataaatcc tcatcagaaa ggttgtctat agtatatttt atagcaagat
    37981 gatctacttc atctgatata acaacagtaa ttcgtaaatt atcatcgtta atgatacagg
    38041 tagttttctt tttacccaat gcggttactg gtaatgactg aattctatga atactttgat
    38101 tatgtatgct atatatatac tgtattttcc agctttcaaa aagtacatcc ggctttttta
    38161 attgtatttt aagttctttt ttatttttcg atacaatcca ttccatagta aacccctact
    38221 ttgatgagca atcaagaatg tgattgtcaa atactgagcc aatcaacagt gatgaattgt
    38281 atcgtacgcc cacagtagaa gattgaatta gtttaccatc atcaagaaat tcagtttctt
    38341 ttttgtagcc actatcagta aacgtaattt taattatttc tgagggggct attgcctgtt
    38401 catttttgga atgctttaca aaagttaata attttggatg gcaacctgcc cataacattc
    38461 cctgcctatc aaacgttata ttatcaacac ctgtattgca ttgtatatca tataaaaacg
    38521 ttaaattata acctttttca atagtatata cttttataca tttacccaca ggtgaggcaa
    38581 caaataattt taacctgtca gggcttaatg ttacaccatt agcatattta atatcttcag
    38641 ctttaatttc aaaatttttc ccattataaa acaatacata cgaattggat agctggagat
    38701 actcctccag tgacttgcca aacgcggatt tgttaccatg atcattagta acgtaaaaca
    38761 tgtcttctgc taccatggca caatcgttgg gtgaaaacat aagattttga tcctctatgg
    38821 attttatgta tttaagagtg ttttctttaa taagaaattt ttcaatataa tgtccatcgg
    38881 tgcggtgatt cactacatac agtaacagtt tatcttttgt aattgcaatt aaatcaattc
    38941 catgaggatg aaattcaaaa gggaaatcag ttttcatttt gttcagtact tttgtttttg
    39001 tattatatga atatattgcc ccgtttatat tctgtccacg tgcataagct ctacgatcat
    39061 cagaagaaat atatgcaatg ccggaattat agtctatgac gatatcttca ggtcctggta
    39121 tagaaccaac aggtttacat gaataatttg aaagagaggt tattgttttg aattcaccag
    39181 catcatataa tgttttgatt acaaaggcaa taactatgag aattaatacg ataattatta
    39241 taattgactt tttcatatac tgaacctccc agaagaaaca tatataaatc tttttttgta
    39301 ataaatgtaa taaacacaaa tactctaata gtatacatta acccttaaaa acagcaaatg
    39361 tatattttta ttcacttcaa atacggtgtc ttatacaaca cttaaatcac ttccctgata
    39421 atagaaagat agtgtatatg tcgtcaagta ttattacaat aaatttttta tgaatattat
    39481 taaattttat ttacatcctt cttcatgcct tcccacatct ctttaagccc ctttttccac
    39541 ctttcatttt ccaatgcttt acctcgccat tttctgcctt caatgacatt gcgttccagt
    39601 atttgccatc catgttcata atcaaaccac ttccactggc atcctgcttt ataaatacat
    39661 tcacaatttt caattttgat taatacctca gaagcttttt cataatcaaa ataaacccag
    39721 tgcatacctg cataaaagat atactccgga tctcctatgt tcaataacgc atcaaatcct
    39781 attttatgat taaagctttt ccagtccaat cccgcatggt atatatactt ggaagagtgt
    39841 aattctataa ggcgggcaag ccctctttca taatcaaact gcttccagaa cctccctgcc
    39901 ctgaaaatat actcgtcatt atcccattca aaaatctgat taatcccacg ttgataatca
    39961 aagtttttcc agtaaatacc tgcatgatac aatccatcaa tatcattatt ttttatgaag
    40021 tattccattg ctaaagaaac atcaaaatat atccagtctt tgccagcttt tacaatataa
    40081 tctgttacat tgcaggctaa aagttcctgg agcagattta acgattgacc attgttttct
    40141 aatcctgccc tgtaaattgt ttgcgctttt ttaatgttat cttctatgtt catatggttt
    40201 gttaaacatc tgtaataatt atgcaatgga attatacaat agaaattgct ttaccaaatg
    40261 gtggtacaaa attttcattg cggctttttg ttattagcca taacgtactg ggatatggct
    40321 tcaatttatc aacatcatat atatatccat ccgtaatgac aagcaccatt tctttatgcc
    40381 cctttaaata ttcattaaaa aatgaagaaa aaaacgttgc accgccatat cctgatttaa
    40441 tcagcttcca gtcacctttt ttgtattcat agggttttgc tgatggttca gatcggatga
    40501 atgtgtctcc ctgttttacc ggaataaaat attcttcatc cacacataca agaaagactt
    40561 tatacttgcc agttaaatct tctataatac caaaagctgt ttccaggtct tccctttgag
    40621 ccatcatgga agcagaaatg tcgatagcta ctgtaatgat ttcactctgt ttatacgccc
    40681 tgcccgctat atatattccc ttagcaacat atcttcggtt aaaccgcgaa tgagtgtaat
    40741 accattcgtt tgaatgcgac aggtagtcaa taatactttt gatatgattt ttataaaatg
    40801 aactatcgca catattaatt ttttccagta atgccactat atccaaatag gatcgttcaa
    40861 cagtacacca ctcatctttt tttgcatgtg caataatttt gtttttcact gaacgcttat
    40921 tttcgttatt ttcggcaaaa taatgaacac ctgttggaag atgcgaattt tcatccttta
    40981 gtattatctt atcatcatcc acggaagaat gtgaaagata gtttttgtaa ttaaattctt
    41041 tatcatgttc ctgactatta ttatgagcta tcgcatacct atcatcatga aacattcctt
    41101 tttgcttttc aatatcacca tgatgttgaa taacctttaa ttcattttgt ttttgtaata
    41161 gccaccgata tacatcttcc cacaccggat catagatagc ggtttcatca taaaactcct
    41221 gtggaataaa tggtaaaccc tgaggaatag tgagcacagg cacatcccac tgatattgcc
    41281 ccttatggga aaagaaagtt ttgctatgat tgctaataaa tgtatttacc accatatcct
    41341 gggcaatatt ctgcaataca atatcacctg tcttagccct gtggtcatgt tccagtataa
    41401 catgaaacat ttcatgaaca agaaggccaa taaattcatc ctgcgaaata gtattcaaaa
    41461 actcaggatt ataatacaaa accattctct gtgatgcaac cagtgataac gttgggattg
    41521 attgtgtgtg tacaaattca acggcgtgat aaaaatatga ggtgaatctg ctctgcttcc
    41581 agattttacg aagtgcacta ttaataaatg aattgtccat tgctattaca catcaagaaa
    41641 aacctgaact ttttccataa tactctgttt ttcttccggg ttatcaatat agtttaatgc
    41701 atccggtatt tgtgtaaaca gcccgcagtc tcttaccagg gaatacataa atgacactcg
    41761 tgtgtcagca cgtgtaaaag atataaattt cagaaaatga gataactttt ttaataattg
    41821 ttcatcaggg ctaccacttc ttttgacatt ttcagtatac agaatatgaa ttgcgccatt
    41881 aacagcccac aatatatcct ctgtgggaat agtattatct ataagttgct gcaatttttt
    41941 ttcatcacct gcaagaatat cattgctgct tatggcacgc ggagatacat actgtaaaat
    42001 aaaatctgtg ccggcaacat cgccaattaa cgatataata attttttgaa gcactttata
    42061 atcattatta aggagataga tcctgaattg ctcctcagtc tggaaattgt acgccttata
    42121 cagagtatct gaaacctgat gccatccgcg tggatttgga ttaacggccg aactatccca
    42181 tagcctgtcc gggtatgttg atatgtactg gataaccgca ggatgaatac cttcttcaat
    42241 tgcccattgc agccagtttg aagcatcagg gattagatta aatattgcca atcttccatc
    42301 aagagctgaa tcatcaaaat cagtaattac ggtattatct gaatcaccta aattacccgc
    42361 aaggacaatt gcagtttttg gtggcaatat atgttcgcca cacatacgct cagttataat
    42421 ctggaataat gtctgaatga cagttgggtg tgctctgttt gcttcatcta aaaaccataa
    42481 aattccatta tacccttctg gcacagtttc gggaggaata aatcgtggcc tgaaataaat
    42541 cattctttta agattgttat cgggtacagg atagccacct aagtccacat tttccttgaa
    42601 tgctaaacgt gtatctatgt aataataatt atgctttttt gcaacctgct ctacaattgc
    42661 tgattttcct acaccaggat acccaactat tttaattgca taatttattg caccttcagt
    42721 ttgcaggtat ctgtccagaa tctcaattaa ttcgatagtt gatacttcta ctggccgttt
    42781 catgataatt aattctatcg taaacactat atttattcaa taatttattt atatagcctc
    42841 acctattgtc acataaacac cggtaaagtt tttgctaaat cctatatcaa ggcgtgcatt
    42901 aagagtctga tctttcttta acttaaatcg caaaccgcca ccccatgtgt agataatatg
    42961 ttctttatta aattcacgta ttgtctgggc aacctcaccc gcaccagcaa aaccacaccc
    43021 aataaacctc cagaataaag gaaatctata ttctgcatga acaacgatta cattacggtc
    43081 cctgtatctt ccttcataca aaccgcgcat aagctttgct cctcccattt gtgacaataa
    43141 ataaaaaggg gcctgaccat gtatataacc agaaataaac tgcaccgcca gaacatgaga
    43201 aaaccagaaa ggttcaaaga aacgataatc aaaggttaca ttaatataat caaaatcact
    43261 tttaatatat tttgaatact gtgtaatggt aaaattggca tatgaaccat cacgtgtaaa
    43321 cagaatgtcg tcacgattgt cataatttat ggtaaagcca agaccggaaa caagtccttt
    43381 atctgctcca ggtaacaaat ttgaatcaat gagtccgcca tcttgagcct gtgttgtttt
    43441 tcttacatca atgtgataca acaaaccaat atagaatcca ggaataaatg cacgttcaat
    43501 aatattttta aaggaaaata ttctggaggt atattcttcc ctgtcatttt ctgtagtatc
    43561 ctggccaatc ccataaaatt tatcaggata attcatatac tggaattcat ttttgatatg
    43621 atatttgtct ctaaaaaaat agaactcagg aaaaacttga aatatgaact gatcattttg
    43681 tgtatgggta aatgttattg ctaatgttgt tggacgcaac tttattggcg gtatgctcat
    43741 tattatatcc tgaaagtatg tcagaaaaac aacaccatag gcaaactgtg tttcaggttt
    43801 ataataaaga tgtggtatta tcagccagtc attctggctt atgacaacta tatcctgtaa
    43861 tggttcacct gttacagtat cttttttgtc agtttttgca aaaacttcag atgaaaaaaa
    43921 aacaaatgat aaaaacagca ttagatataa actgattttc gtagattttc taaagataga
    43981 tttattcata attactatta tagacataaa catgaatcta ttcctaaaaa atagaacgta
    44041 tggtattcat aacagttact tttattttat cttaatccag taatatatta atcctgtaga
    44101 agttaagatt attcctaaca gtgaaggcat aaatgcagta atcaatgtaa ctatcaaata
    44161 tattacataa aataaaacta ccagaattac agtcacggga tatccccacg tcttaaatgg
    44221 ccttacagcc tcagggtatt ttctacgtaa aacaattacg cttgttagag tcaatatatt
    44281 aaaaatagtt cctgttgctg aaaaataatc aataactgct tcataagaat ttttgtatat
    44341 tgatgcaaaa agtacaagta cacacgccca tacaccctgc ataatcattg cattattagg
    44401 ggttttatat gtagtatgga catgtgcaca ccatgaaaaa aacaaattat cctgtgccat
    44461 ggcttgccat gtacgcgcct ttaccataac ctgcgtgcca acattgccaa atgtatttat
    44521 cattactgca taagcaacaa gtaatgcccc cgtagcaccc agtgcagtct gtaatgcatc
    44581 aactgctatc cattttgaaa ttgcaaattt atctaccggt acctgaaaca gatacgctgc
    44641 attggcactc aaatagagaa tcaaaacaca cgcaatgcca atcagtaaag acattggcaa
    44701 attttttctg ggattcttga cctcttcggc aacatacgtt gccccttccc aaccactgaa
    44761 tgcaaaaaat gcataccgta aggcagctcc aattcccaga acatgtgtca atgtaaaatg
    44821 ctctggtaaa agcggtgtat agaaattact aacattacca ttttttgtaa aacacgctgt
    44881 tataatataa ccaagcgcaa tgatttttga tgaactgata ataatttgta caatcccact
    44941 gagtctaaca ccaaaacaat taataaaggt atgaaacact attataagta aagcaatgat
    45001 aacaaccata gcatgtgata gatatatccc tgaagaaatt tcaagggaag cctgagtata
    45061 ttcggcaaag acaagtgcaa cagcagcaat agctcctgtt tcagatacaa agaacattgc
    45121 ccacccacgt aaaaatgcaa tcaaaggacc atatgcttct tttaaataga tataaggccc
    45181 gccagaccgt ggcatcattg ctaccagttc agcatagcaa actgcagcaa aaattgtaat
    45241 aactgcacca ataatccata cgcttccaaa aagcatagtt gatcccacaa gcgccattat
    45301 gggtccgggt gttctgaaga ttcccgatcc aattatgcga cttattacaa tagaaacagc
    45361 tgaaggtaac cccaatccac gggtaaggtt tgtatcttca gtaaaattct tattttggtt
    45421 gatatgcatg atggttattg cgcaggatgg taggtatagt tatgctttat gtatatatct
    45481 tcaactttaa aatcattaga acgcaataga tcaaataact cccttgcttg cttaaggcta
    45541 gataatccta ttaccactcc gcaatgcctg gttattgaat tgggaacagg taataaagca
    45601 tatgataatt caggatgatt gttctgtaac cagtaatggg cacgcattcc ttcggcaacc
    45661 gacacgaatg tgattaagca ttgggttgta tcttttctga aaagagaaac aaaaattttg
    45721 cgaatatgca atcgtacacc taatagataa aaagcaatta aatcacataa tgattgtggt
    45781 ttatatcctt ttcctttaat tatttcaaat cgggcagtta aatgatcaac tactacggta
    45841 ttcatgatat tacccgattc aacacaccat tgctctatct cttttttcat ggaggggtca
    45901 tctgaaataa tctctacaga tgagccatca ctgacagtct tcatcgtttt agcaattaat
    45961 gcaacagata taggacaact atagccacga gtatctataa ctgttttttc actttgatct
    46021 ttagccataa aactatgacc tgctagagta taccttaatc atgatatgca tactgcaaca
    46081 tgcttctgta tttattatac gtatagtacc tgaactttaa tatataatag cggttggtaa
    46141 tcagttttga atttttaaaa tcatacggcg aataaagtat tggatttgcc agaattgcta
    46201 acaatcgtga tttttcatca gtgcttagtt gatatatatt tcttccaaaa tagtaatatg
    46261 atgcattttt aatgccaaaa actcctttgc ctaactcaca atagttaaga tataattcaa
    46321 gtattctatc cttatccaga atcatttcca tttccagtgc tatcaacagt tcaacatatt
    46381 tccttataat ggatttagct ggtagcaaaa acagagtgcg cgatagttgc tgggttatag
    46441 tgcttgcgcc atacattctg taaccaactt tataattaat aactatcgcc cgtttgatag
    46501 cttcaatatc aataccatta tgtttataaa acttgtaatc ctcaattgag acaaccatct
    46561 tcttgatgtc atcaggaatt ttctgaaatg ggataaattt taccggttta accttgtaac
    46621 catcaacata caaccggtat gccattaacg gtgttgtata tgggttatga tgagaataat
    46681 acgatagtaa agcccccata gccagaacaa aggcaatatg aaaaactatc gccactttaa
    46741 taattttttt tgtaaaagcc catttatagt tcagagattc cagatagtaa tcagtaccat
    46801 tactccagcg ttttaaacgc ccatacatca tttttaaatc ctgttccatc aaaaccacct
    46861 attaaaataa ttctgtcctt aaatacagta caactgtgtg aaacacgctc acccatcggc
    46921 gtaaaactgt taaaaagttc ccaatccttt ccattgtctg acctgtaaac agtaccccct
    46981 tttctgaatc cacctataac ccacaacgat tttttatata ccactgctcc atgcccaccg
    47041 ccagcaaaaa aatgcatatt gcttgcaact tttttccagt gaattccatc atatgaattc
    47101 cacacatcgc tgaagcttgt aaaattatta tttccgtcaa cacgaatacc accaataacc
    47161 cataatttat tgttgaatgc agtcaaaaca tgtcctccac gtgaaccaaa gggcaaatta
    47221 cctgcaattt tatgccagtt aataccatcg tctgatttaa aagcttctgc accacaggca
    47281 atacccccaa ccagcaaaat agtattgtta aatactgtac atgcatgtcc accacggggc
    47341 ataaattcag cggtttcttt tacacactgc cagctaatac catcttctgt tgcccatata
    47401 tcattcaggt caataatatt tcgtgccgca tcaaacgtaa gcccgccaac tatatacaat
    47461 ttatcattat atacaaatgc agtatgtcct gctcttggtt taaaaggcat tggcccggca
    47521 ctataccagt gtaaaccctc ttttgaatac catacatcat tatacggatt attgccgtcc
    47581 cagccaccta taagccataa tttacctttg aagacagtac aggtatgacc ggctctaccg
    47641 gaaaatggcg catgcaatag atatcgagta aagcttgatt ctttttcatt aaaaccaatt
    47701 agcagtgaag tagcaaacac aatcagccct gtaattaaca gtactgttac accatattta
    47761 tataaaattt tgaaattcat acttattctc tgagattacc tgacaatatt attcaatttt
    47821 ctattacagt cttttttatc gctatattat attttttcaa tttattatgc attgttttgc
    47881 gtgatacatt aatggccttt gctgttaagg aaatattata atcaaaatgt ttcaacattt
    47941 tttcaatata ttctctttcc atttcgtcaa gtgttttaac cctatcaacg tgactttcgc
    48001 tatcctgcac ttttttgcta tttacaaaag cctcaggaat tgtatggttt atgtgttcgt
    48061 tttcaacatt cacggttttt tcttttggtt tattatattc ttcaaattct aacacacttt
    48121 taacaatatc aagcttaaca acatgttccg ttgaaagcaa cacaagcctt ttaataacat
    48181 tttccagttg ccgcacattg ccaggccagt gataatctgt caaatataat cgtgattgca
    48241 aatcaagtga aatatcccga ccatattcag ttgtaaattt attcaggaag taatcgataa
    48301 aaagtggtat atcttccttt cgctctttca acggcggcaa ataaattggc accacattta
    48361 aacggtaata taaatcttcc ctgaatgtac cctgcttaac catctcgcgt atgtcacgat
    48421 tacttgcagc aacaattcgt gtatccactt taataatttc gttgccgcca atacgctcaa
    48481 attctttttc ctgcaatact cgtaataatt ttgcctgcat atcaaatgaa aggttgctta
    48541 tctcatccag aaaaagagtc cctttgctgg caacttcaaa cttaccaatt tttcgggcga
    48601 tagctcctgt gaatgctccc ttttcatggc caaaaagctc actacctatc aaatcctgtg
    48661 gtattgtgct gcagtcaatt gatttgaaag gatattgttt gcgattgcta ttataatgaa
    48721 ttgcttttgc aattatctct ttaccagttc cactttcacc aataattaaa atggtactat
    48781 cggtatcaat aacccgttcc aacgttgcaa agatacgctg catttttggc gacttgccaa
    48841 taatatttct aaacctgaag ctttctccaa ctttttcgcg aagctcattg acttcgtttt
    48901 ccagatcaag cttttcaact atatttttga gtattaccag aagtttatcg gtatcaaatg
    48961 gttttaccag ataatcatag gctcctaatt tcatggacgt aattgccagc tgcacatcgt
    49021 ttgaagcggt gattaccact actacaatat ccaattttaa atcacgtatt gtctgtaaca
    49081 cctcgatgcc gtttttacca ggcatctgca gatcaaggat taatatatca tatttatttt
    49141 ttgttatttc ctcaattatg gtatcaccac gatcaatggt actgacataa aatgagcctt
    49201 cgagcaggtc ttttaacagg tctcgaatac ctttttcatc atcagcaata agcactcggt
    49261 aatatttttc aagaatggtg ttcatggtca cagcattaat tatttatgaa gaatagttac
    49321 attggaatct caagagtaaa tccattacgc tcttttaaaa atgtaaattg ccatccactg
    49381 cgctgtgcaa tgcaatacac tttaaaaagc tccgtcccct tactgaaaaa ctgcttacac
    49441 tgaaatggat aataaaaaat atcttcaggg atactatcaa acacaacacc tgaaaaactg
    49501 atattgacaa tacagtgcat atcttttaag gcattaatat gtaacgatgc accagaaaaa
    49561 tatttccagt taaaaccatt aatgatgctg ctcaggcatt gttgaaatct attgacatac
    49621 agtgatatcc tgaattgggc attgcgctca attgtaatat actcactata tggagattct
    49681 tcaaccaccg tatacaaaac ttcaggaaac gtgcactctg cacgatcatc atctatcaca
    49741 gtaaatgcat catacaactg tgtaaaaata gtatcaatgc gttcagattg ctcaataatc
    49801 atatgctgtg acttaacaat taaggcaggt tcattttttt gcgatagtaa ctgggcatat
    49861 cccttcactg tggttaaagg gttgcgcact tcatgagcaa tatttcgtat tactcgttca
    49921 atgatatcaa cttcgtgcat gtgtgattta atctaattca atctgcttag catccgccca
    49981 tagacgctct aactgataaa attcacgtgt ttcctttgta aatatatgaa taataatatc
    50041 attataatcc agaacaatcc atggtgaatt ggttgtggtt cttgaacgct ctttaatgcc
    50101 cttttcaaga aaatatttat gaacttcacg tgccagtgcc ttgcaatgta atatagaatt
    50161 accggtacat attaaaaaat aatctaaata ggaattcaca tcccttaaat ccagaaagac
    50221 gatatcaatt gcttttgtat catcaagtac ctttttgcat cctttcatca gggataaaac
    50281 ttcgggatct accgtaattt ttgattttgt tcttttactt ttacctgtca ctgtgaataa
    50341 tcctttccta caattactaa gacatctgct aactgcgatg agtcaactat atgatacatg
    50401 gtatcaattc caagaattga agatactttc aatgctgagt ttaaattccc tttttgattt
    50461 attatcacag ttttatcagt tacctcatat ggtgatgtgc caaactcaac cactgtaagc
    50521 ccttcacgcg tcaatacatt gcgcatcttt cgagcaagcc cgggaatatc tgagccattt
    50581 aatattctga ccttgattga ttctgaatct ttaatatcaa gaaccagtgg gcgtaaaaac
    50641 ttttgttcat ataattttct ggtaatatca tccaccgtat aattattgtc cttgtccatc
    50701 ttgcctggca taattgttgt atatacagtc ctggcatcat acacaacatc aatcagggat
    50761 attatttcct gtaataataa attggtattt attgaactta ccatttcact gatatattct
    50821 ttttttgcat attttttgta cacatcttta tttgtaaaaa gggtaagggc aacatccatt
    50881 attctatcat attttagata tattgaattt tgttcaactt tattaatata ccataaggcc
    50941 ttacggccat cacagtaatt aatgccagtt gtatatccca aatccatttt cacctggtca
    51001 agtatatata aatttattcc ttcaattaaa tcaataattt ttatggcatc tgaagcatac
    51061 aattcaatgt aaaatgggat tgtaatatga aggtcatctt caagtgagtc tattatatca
    51121 ctgtaatcag aaaacgaaag tgactcaagc ctttttgctt ttccggttct tttgttaaga
    51181 gtcaccagat aatttggcgg aacaaatgta acacctatac tttttttttc aggattaaaa
    51241 tggagaattg catagaacga atgccgcctt tcattatatt cattgcttgc agtcacaaga
    51301 atattaatca attctttatt ggcagccagt gtttctatgg cattacgtgt aaatctgcga
    51361 tacacataaa atatacccat tactactatg cagatcaatg ttatagaaac aattaaacgt
    51421 attttatctt tcacttttat ataacccatg cttatagata tattgttcta ccctgcctgg
    51481 taataaatac ttgatagatt tgccatttct taccctgtcc cgtataatag aagaactaac
    51541 ctcaatgaca ggattttttg cacagtgaat gcgtgacata tatttgttaa aacgtttatg
    51601 cagtacatcc ccgggacgtt tcattatgat gaaatcaatg gtcataagca gacgcttcca
    51661 ttccttccat gtgtttattt cattgaatgc atcatagcca ataataaaaa acaattccca
    51721 gtcagggttt ttctgcaata tttcttcaac ggtataaata gtataggatg gcttttctct
    51781 gtcaatctca agccgtgaaa ctttaaaata atcaacacct tcaatggcca atctaatcat
    51841 ctgataacga tgttctgctg acacattatc ttctaattcc ttatgaactg gatactttgt
    51901 tggaatgaat acaataaaat ctaaattgaa ttcttcgcgt ataaactgag cattaatgag
    51961 atgtcctata tgaacaggat taaacgtacc accaaatatt ccaaccttca aatgagcctc
    52021 accttctaaa cgtatttgat tcatctttat gaatgcagtg tagcagtcaa gaaattttat
    52081 taatcagtat gaatccactg ggcatcaacc ttgtgttttt gcgcaaaact atctaatttt
    52141 tcgggataat ggtgttttac tatttttacc atttgttttt ttaaggtttt gtcagatagc
    52201 tcatatactt catcaaaaaa ttgcaaaaat tctttgcctc catataaagc taaaatattc
    52261 aaaatgactc ttttggatgc aatattttct tcctgtttgt agcgttcaaa taaaaaaggg
    52321 tgatctatta tatgtaattc atataatgca caataccctg ttaatctttc tatgggattg
    52381 ttgctgtata gtttattgtt gatagcagag tacgtataca atggctgtgt tataaagaat
    52441 gatattgcag tactccctga taccgtttta gcaacaagca atttccctaa aataaaacct
    52501 gcagccatac ttgtacataa tatgaacgca atccttatga acttctgctt gcttatcatt
    52561 ttttaaaagc ccttgataga atcatgaata aatgagctac agctactatc tgcctatacg
    52621 ctataaataa tgaattatct tgtcaataat gattaatatt gtttacattt aaatagaaaa
    52681 ggtaaacctg aattgcaatc cctttttctt ttcagtatta aatgttatta tagcttcata
    52741 gttacctttg ttcttcaacg aaacatcaga tccatagtga tttttcatgt gaaaaagcgt
    52801 atacgttttg ctgatatttt tgcttttaat atctactgta gcacttttaa tagttcctgc
    52861 atctttacct tctacaaaaa cagacatatg atgggtaata tccggatcaa gtttgctggc
    52921 atcaaatttc atcccacttt cttttatcat ctgttccatg gcggttttca catcattcaa
    52981 atacacttct atagataatc catccttttg tgcttgaaat accattgtac ccatagcttt
    53041 atgagttgat ttcccacctt catgcccctg atgcatgtgt tgggactgac caaaagatac
    53101 atacatcatt aacaacataa ctgttaaaca aactattgat tttttcatat aatcctccta
    53161 taaatttatt aatgtaaatt aagttcacgc cttttccaga gataatatat tgcaggatat
    53221 acaagcaact ccataataaa ggaagtggtt aatcctccaa ccataggagc agcaattcgt
    53281 ttcatcatat cagcacctgt tcccgtagac cacat
//
LOCUS       DAORYL010000010        53103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_525_length_53103_cov_14.050313, whole genome shotgun sequence.
ACCESSION   DAORYL010000010 DAORYL010000000
VERSION     DAORYL010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 53103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53103
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_525_length_53103_cov_14.050313"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..1308
                     /locus_tag="PLV81_03605"
     CDS             238..1308
                     /locus_tag="PLV81_03605"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HOM09221.1"
                     /translation="MRYIGVDLHKNRFTVYNATTGRYRDYGTNKVGIASFIEECKRTE
                     GTGAIVVGVESTGNTRYFKRQLEEEGIQVKVINTMKFKVITESVKKTDKHDAQTIAEF
                     LEKDMLPEAWICSDYSEMLRRLLHIRRQLVKAIVTTKNQVHSILTAYGYEDARATLQS
                     KRGRQAVLDTLGSTEIGLVVHLLIEMIDSLDEKVKQIEGKIEELVKGDKAIELLQTMP
                     GCGRITAWMIRAYTDDIRRFRSAKHYAAFVGVVPYVHNSNENIRHGKITKHGPELLRT
                     AFVQLVMGMRRIKQVQDYRLMKRYKALQRTKGSGKAIVATARKLTHIVWHILTYNEPF
                     DASLQMVSGYENNRSMQEKQLA"
     gene            1381..2034
                     /locus_tag="PLV81_03610"
     CDS             1381..2034
                     /locus_tag="PLV81_03610"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOM09222.1"
                     /translation="MSDYIAFYDLDHTILASSSGTLIVRDLYKRGMVSLLSLINGMLL
                     SLLYKSGIINTQSLIMHWVKRLKGMEAKTIEQYSEIFFEETVKHYIYPQALESIYMHK
                     AKGASIVLLSASLDFICKPIMKHLHFDDVLCTELEVNDGYYTGSIKGNYCYGIEKRNR
                     AMQFCTDHNIDIKSAYYYADSVADIPVMEAVGFPQCVNPQPALLKLAHVKGWPVHRW"
     gene            complement(2036..3682)
                     /locus_tag="PLV81_03615"
     CDS             complement(2036..3682)
                     /locus_tag="PLV81_03615"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004397381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOM09223.1"
                     /translation="MKIAIAQINPIIADIEGNCNKISQFVTLAKEKGADLVIFPEMAT
                     IGYPPMDLLEHKKLIDDNLRALEAIAPLSSDCGIICGYVDYNYDNPPMLYNAAAFMYD
                     GKLQSKHYKTLLPTYDVFDELRYFSPAPSQEIISYKGINIGITICEDIWNDMSLSEDM
                     FMEFRRYAVDPIENCMKKGADILINISASPYVKGKNSIKWSKIQHVAKKYKVPIIYVN
                     QVGGNDSLIFDGNSFVIDKKGNFVLKAKNFQEDCILFDTEKEYMHISCEEDFIADIEK
                     ALILGIRDYVYKSGFKKVVLGLSGGIDSALTACLAVKALGPENVLGITMPSKFSSKGS
                     VDDSVELAHNLGINIHTIPIKNLFETYIHELNPFFKDLPFDIAEENIQARIRGNILMA
                     FSNKFGMLLLTTGNKSELSMGYCTLYGDMAGGLAVLSDVPKTLVYALSYYINKDKEII
                     PRSILEKAPSAELRENQKDQDSLPPYEILDQIIERYVELKMSAEQIIADGFDPDIVYS
                     VLRTIDRNEYKRKQAPVGLKVTTKAFGVGRRIPIVQRFKH"
     gene            complement(3724..5001)
                     /locus_tag="PLV81_03620"
     CDS             complement(3724..5001)
                     /locus_tag="PLV81_03620"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOM09224.1"
                     /translation="MKKTGIIVVFIFIAYTVSFASDECIKTLVNNVDDSYSNLSDECI
                     MKLKELIVKGKDNDQKIQALQILKKSKVRTNIEFFKDILYSVRSITVQQMVIEILAEM
                     HDKKVLPVIIEFLPSPFFAVRESVILALKKNGDDRMYPYIIRLARSDEVIHRIYALEA
                     LYHLYDNRFYEIIVRLLQDTNKSVRYFALDCVVHNEISAALPYVRSIASGDENEEVRV
                     KAINVLSALHDRAAYYIFVRALSDYSDLVRDAAVDALSTLLSKESTIYLSRQLLTERE
                     VYIKRKIIKALITAKNAGDIRGLQHIVHNEENRSLRMYAVYALGVIRDKRTVPLIIES
                     LDDEDFRIRAEAAAALSQFKGKNVVIPLLESYSKENEMYVKTAIVFSLKKICDMGSLQ
                     KLFIIYTQEQDIVLKMILSSAIQHIITRNTRQY"
     gene            complement(5118..5726)
                     /locus_tag="PLV81_03625"
     CDS             complement(5118..5726)
                     /locus_tag="PLV81_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOM09225.1"
                     /translation="MPAKGQKRRQQIVDIAKKMFIEKGFQSTHIGQVCEELNIARGTV
                     YQYFGNKREILYAILESIEESIDDILDPDDLRDLLKDNPSQKDIIKFNTDRITECIKA
                     ILSEPIVIKLIFKDIVGIDEEVIERVDQFLKHLINIVSRDIEEIKKKGLYKKTLNAEI
                     TAIMLIGSVMFLVYQFDKEKKDVTDKEVINSLVTHYFSGVLK"
     gene            complement(6132..6824)
                     /locus_tag="PLV81_03630"
     CDS             complement(6132..6824)
                     /locus_tag="PLV81_03630"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM09226.1"
                     /translation="MEELDIHKQIKVLLDQATHLLNHKKDFGQAKDTLHKLESMDSEN
                     PVVLYNLAIVCIHLQDYNSAIDYLKRCMSLPMTFIDIRQVRKVYIFTLIQKGDYDRAL
                     DELQQQNDQDDDIIQMKAFALEKKGNYRQALQLYENILEHNPDNMNACNAIAYIIALL
                     PDGNLSRALELAKKAVSHVPNNAAYNDTLGYVYYKRGEYNMAKKFLKKALSLKPDNSE
                     IRSHIDELLNIS"
     gene            complement(6812..7570)
                     /gene="surE"
                     /locus_tag="PLV81_03635"
     CDS             complement(6812..7570)
                     /gene="surE"
                     /locus_tag="PLV81_03635"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOM09227.1"
                     /translation="MNILITNDDGIHAKGLQVLKKVLSDNYSVYAIAPDKERSGCSNA
                     IQIRKSIKVEQIDEFTFAVDGFTADCVNIGLHGGLIPPVDLVISGVNHGPNLGDDIYF
                     SGTVAGARTAYIFGVSGIALSIDSYEESDYFYDASWFLLELLKDFPLHSKEPLFLNIN
                     YPDLPRSAIKGVQYTSLGKRFYRDSYRIIQRNNNYFDMELDGTIENDEKTGSDVTELR
                     NGYIVITPLTIDCTDYNFIDRLKKREIKNHIWKN"
     gene            complement(7900..9000)
                     /locus_tag="PLV81_03640"
     CDS             complement(7900..9000)
                     /locus_tag="PLV81_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOM09228.1"
                     /translation="MIQKAYIHSLHGYHTEKKICGLYILERLIYTLQKVGIKQIYLML
                     NEQEKILYEHKILPHIKKRKINAHQCLPGEVPEKGSLYVPSNLFVQPHYFEHFDDYFK
                     QKKGVFEPVLNDNQFIISTDEDIKKAELLVVKYIIDNTGGYIAKNINKRISIPISLQL
                     AKTRIHPNVLTIINMIVGFLSAYFVLQGSYWQVVLGGLLFQLASVFDGVDGEVAKFTF
                     KVSKIGGWLDTIGDNGTLLLFLIAVSVLYFENTSARTASIIITLLFVGLFWFIGMIVW
                     YLRKFSESGSLVAYDKEFLQKLPQDDVLVKFALSMKYFTKKEFFALFFFCISFTGKIY
                     VLIPIITFTVCCGAIILTIINKKYFRIVNTLR"
     gene            9218..9877
                     /locus_tag="PLV81_03645"
     CDS             9218..9877
                     /locus_tag="PLV81_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09229.1"
                     /translation="MKYVNFFLSIIFLTICLILYSAPQAQENPEKQFNPLYDAEAVIH
                     RLSYDNFKNIKLLRTAIYNFGGGEQEFNSLVDTYAEASALYFRNEMIASANLFTKNEK
                     DIKEVAMRLAKLYKEQAEKLHIQVIKMGVRHSLKASIEQTKPNPSVDPLISNASYGIK
                     IANDYLVRSKPIDAIYYFRRAKENCFKVYQVLGEQLPEEYKKDIVDNQNKIYIAKEKK
                     N"
     assembly_gap    9909..10008
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10009..10307)
                     /locus_tag="PLV81_03650"
     CDS             complement(<10009..10307)
                     /locus_tag="PLV81_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOM09230.1"
                     /translation="MTSSCGKKIFNGLMIWLLSVLIGSCNSIKPITGYDSVLVYYENG
                     YKSDNWKTRLEAIQAVATIKGERADNLIIQALHDPHNAIKIEALQILSKRPIKRAR"
     gene            complement(10276..10971)
                     /locus_tag="PLV81_03655"
     CDS             complement(10276..10971)
                     /locus_tag="PLV81_03655"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOM09231.1"
                     /translation="MKQCIRKSVIIVLILFFTANAIAKPVGKILDLSGDVDITSVSGK
                     RIIPNEGTILEDTDKIRTGRKSFVSVLLNDGSKLFIREISVLYVQDIRLKEAEQPTKI
                     QMVTGKLRIIAAKTIKGTSVIVYTPTAIAGVRGTDFGIIATQFETKVVVFKGEVEVAN
                     RDTKILKSYILKDREETRVAYQQPPEEPITVPDYMLPKWFDSYVIDEKRNIIIKIKQE
                     EGLIDQLLRKKDF"
     gene            complement(11179..13491)
                     /locus_tag="PLV81_03660"
     CDS             complement(11179..13491)
                     /locus_tag="PLV81_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOM09232.1"
                     /translation="MIIRTYTLMVTLLTLAIFSFLSCSDVVLPQGLSELGFYSNNKTI
                     IQTLEQKGGLSYTEHFMLGLAYKEAKDYKKALYHFANSCFVERRARKLSIYPQPVYRF
                     VSRFSVKSYLYNDAMYEIAKGFMYYQEYEYVIKFIDLMKHEKTGVFRDAQILKAEALG
                     QLKQFDKAEKVLQKSISKYNDRDSLAWLYIKLASLYEKQDKNTDAVNSYYNLLKISTA
                     SWQSEIAAKRILQITTKNPDIALNPDEIQLIIEALYNTKNYQDIVSIDENLLQLVTES
                     TQKLHLDMIRVRTLCHLGKWDTLKTIYDAYQATDYRYDIAATIVDTLWDNDKKGQAMQ
                     WLAKLENADNPKFKQKVLYYKALYAVQRKETDPKPVDEYVAHYPDDTATQELLWLFSK
                     NTITNTKTALYYLEKYYSTFGTNGKYADEVCFWLYNLKKEENPDEAEAIAYSMVAFLP
                     HSAYTWILLERLSPNYSVEKLSSDFSMALQTNNLTATLFAHAMLTYKERDNKKRLQRL
                     DMIKKYFPKSREMQYTTIPTRNEKLSLHIPYEIVTRIEHYFSMGYSEGINRELAAYNN
                     PDNNDVYVLQYIFGLKYKNYFLSAQGLVQLLKRLDMKENIFLMHNDIIQSILPKPFVQ
                     CVSQSSKKYDIPEPFIYAVMKAESLFNPFAESSAGAIGLMQLMMPTAKEIAKKTGVGQ
                     VNKEMLKNPCMSIELGTYYISWLMKNLKNDITLVSAAYNAGIGNVLKWHYDNEDMFTV
                     MAPFAETKGYIERIKKFYYQYQLVYDLSRQ"
     gene            complement(13494..14327)
                     /locus_tag="PLV81_03665"
     CDS             complement(13494..14327)
                     /locus_tag="PLV81_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09233.1"
                     /translation="MRAIISIIIFSIVVVMLLPLQNTLIAQRTKSSQSAGEQRKAEKG
                     LKDNERFFYFINFSVSNVSVEEERIICRKAILYDMFAKFLYMKFKFKDSYENIRKTQE
                     LLISLYRMVLHREIDEGKKLLDTVSPGPILKDDYVSKHYCNLGYTNNEQSRMYMVMAD
                     SYRTTLYSMRLYKYIQALKKAKQAKRYALLAYIAFNHKDSNSRPENKQNVGNEPYIYS
                     YMKTKLESVKDENEKNYMLKVLADSYYRLIDNYSFYDDVWSNGSLQELPEYADYQKEE
                     E"
     gene            14458..15429
                     /locus_tag="PLV81_03670"
     CDS             14458..15429
                     /locus_tag="PLV81_03670"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HOM09234.1"
                     /translation="MKTRTTLLLVSIISLLFYITCTTFDPSMLQKKPTVTFKDFQFKE
                     ITLNDMTMLFSLDVENPYPVTLYLDSIQSQIYIDTFSLFSLQSADKLKLSRNSKTNKT
                     FAVTITYDQLIKAVKEYYNKDSIVITIKGTIAFSLPPSIQIVQSIAVPFTIKKEIPTI
                     KPTIDIAHFKLIPPSLSDLKNIIKNINPLEIPSLYTDIQNFCQGKGVPDSISKFDIPL
                     SVAFDIIIKNKTKAALVGKSLNYNFILHDSEIAKGQPTIQNADGASKINCVTTLSTKN
                     ISKGLTQAIAQKNIQYTMKGNMLFNAALKGTELPVPFTINQQGTIKW"
     gene            15522..16589
                     /locus_tag="PLV81_03675"
     CDS             15522..16589
                     /locus_tag="PLV81_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOM09235.1"
                     /translation="MNVIPVILAGGSGTRLWPLSNEEKPKQFHNITGEGTLLELTINR
                     LKPLNPDFCLIVTARMYEEMSYDELKKTGLKGSIISEPMPRNTAPAILYAATYLSKLY
                     DDAIMLILPADHYIKKTDVFVTILKKAIEHAHNNHLVTIGITPTYPETGYGYIKAYDD
                     PSEVKPVEMFVEKPNLETAKKYLREGNYFWNSGIFVWKISVILEYYQKLMPKLYQAFQ
                     PLRDLTVEQLTQNDELPWEIKRNVFSQIEAISIDYGIMEKADQRYVIPGEFGWADLGS
                     WASIDDVLSPNADMNRSPYPEKVIFHLSKNCTVFPDTKLIAVVGLHDVVVVDSGNEIL
                     VMDKNASQDLREVVNRIKNNR"
     gene            complement(16573..17301)
                     /locus_tag="PLV81_03680"
     CDS             complement(16573..17301)
                     /locus_tag="PLV81_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09236.1"
                     /translation="MNASNKISLCQPAQGKGCCVCCGLFNCADISFAGLEHFLSDATA
                     EIRYSLVTGNIVRNFELLQKQYTFRETTTYVCPYMGFIDSNTPGCLMHPAVNNGIDRR
                     DCSLFGKEICNDYFCPAYTLLDDRLKKILVRYTDNWYTYSIGIIDPLSFIWIVQLIED
                     KLGEPIVPEKSTPDSIVVTLVNTSLMMVADFFNTVPVPVFYYSEPEYHLHMRELSLNQ
                     SSSLNAGVRGKIKNAISAVFNDYS"
     gene            17384..18022
                     /locus_tag="PLV81_03685"
     CDS             17384..18022
                     /locus_tag="PLV81_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOM09237.1"
                     /translation="MKYRDNLYELLDVSPLSSKEEIRRAFCTKVKALHPDSTGLSSER
                     FTVELQKVIEAYRILMDDRERAHYDATLLQYPKKLEKHYIPQKRIRYSISLKDLLKKE
                     FLPKKMRRKDIIHEFDHDIEIVCTQQEISKGAVALVQLPSRILCPLCNGQHRDCYVCN
                     GLGRISSASTFEIELPADITKTTILTVDLRKYRPGTFTDLTIKYLRIKVTIV"
     gene            complement(18030..19325)
                     /gene="hisD"
                     /locus_tag="PLV81_03690"
     CDS             complement(18030..19325)
                     /gene="hisD"
                     /locus_tag="PLV81_03690"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOM09238.1"
                     /translation="MIAIRKLSDLTQKERNTLFQRFGDDFTGIMVNNVIPIVQKVRDE
                     GDKAVIEFTNKFDGVTLHEVCATEKEITAAYHNTDAKVIDSFTRAIENIREFHLHQKR
                     DSFIYTREDGTTLGMLYQPVESAAVYVPGGKASYPSSVLMGVIPAQIAGCKHISIITP
                     PSKDGAIASYIGAMAHILGITTIIKSGGAQGIAAAAFGTQTVPKADIIVGPGNIYVTA
                     AKAYLFSQGVIQIDSLAGPSEVLIIADEQANPHWVAWDLLSQAEHEEMAIAILVTTSE
                     SLAQKVITYIEEDINSGRGRHEIKKQAIQNAHIILVSSIEEAIYFSNNYGPEHMELMV
                     ENPLHYLTQIKNVGSLFLGAYAPVAVGDYFSGTNHVLPTGGAARFSSGLGVETFMRRT
                     SFQYLTPSALQQATEHVSRMSQVEGFDDKHGDSLRIRFR"
     gene            19434..19697
                     /locus_tag="PLV81_03695"
     CDS             19434..19697
                     /locus_tag="PLV81_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09239.1"
                     /translation="MFKHIAVIMIAIALVITGCKKTTITPKLFVTIQNEILDSDMQQT
                     TKEDIVKKYGITLQQYEEYEKQVESDPELKALVGKERLNIMKK"
     gene            complement(19700..20521)
                     /locus_tag="PLV81_03700"
     CDS             complement(19700..20521)
                     /locus_tag="PLV81_03700"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOM09240.1"
                     /translation="MIIDGHTHIFTNDMVQNKERYCNDRQFSLLYYGKARIAHAEMLV
                     ESMEHNGVDASLVLGFTWQKEEYAQMHNEYLATVQHTYKGKIYAFGAIALNGDVYKQI
                     ESIKKLGLYGIGEVAFYANGFNEEEEMLLGQILAIAEKFTLPVCLHVNEPVGHIYAGK
                     YYTDMRSLFALCSQYTNIPIICSHWGGGLLFYELMPEVKKSLTHVYYDTAATPFLYDS
                     GIYDIAIQMCGKEKILFGSDFPLLEYSRYKQHIEALGESAEYVLYKNVETVLGIA"
     gene            20601..22085
                     /locus_tag="PLV81_03705"
     CDS             20601..22085
                     /locus_tag="PLV81_03705"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOM09241.1"
                     /translation="MNTIEFFLEPSKENYHTLRVKAEDGSFRYIHSKYYPTKESSYLQ
                     DIVNPEKYDACIILGIGLGYHLKYLATIIPFFKQIICIDINNYLTNANIPQDIQAIIH
                     HPSVQLVFVNPDNLQQLRDTIQLQFSKGIQVIDHPASFHAFPHIFSSIKKIIQQIVDK
                     SVSNHLTSSLFAYRYFKNALLNLNQLPECFAVTQCKGIHAHQPCIIVAPGPSLDETLY
                     HLKKHHNCIIISVDSALKALYDHSIIPDYIVSIDPQPVVFAHIFPTQPTVPVITTLTA
                     HPMTFSLPAIVSLNTHPVCQLLEELYPGLIGSVDSRTGTVLGDAVTFAIFTGCNPIAI
                     AGADFSFPKCVTYARSTEYHYRYQLMHNRYTPLETKNMDYIIHASKRTRTTTFSRKNF
                     IQYHDAVDAILPKDTSLFHINPVLPLAHAKSIAIQDFLSLPQVSHYTKKMTARLLQPL
                     SHSINLNEIYTILNNETMFNTIVNSSQVHNQKSIHRIHYLLHKE"
     gene            22090..22680
                     /gene="coaE"
                     /locus_tag="PLV81_03710"
     CDS             22090..22680
                     /gene="coaE"
                     /locus_tag="PLV81_03710"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOM09242.1"
                     /translation="MRIGVTGIYASGKGTVCSMFEKLGAIVIDTDIIAREIVNPPSPV
                     LDTLVHTFGHDILNTDGTLNRRYLAQKVFNDKMLVEKLNSITHPAILQEVMQRSHDSS
                     KIYMINTPLLFETEYHKHMDKNIVVVANTEQVLKRGVIRDGITEDEIKARLNHQISLK
                     EKMKLADYIIDNSGSLENTKKQVEELWKTLILMKGQ"
     gene            22647..23417
                     /locus_tag="PLV81_03715"
     CDS             22647..23417
                     /locus_tag="PLV81_03715"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOM09243.1"
                     /translation="MENFDFNERPVAREKNVFLLHLDVPRIILISAVLIGIIVTTFLL
                     GMNFSENKVVSHNTLVPPEIPGDTIAQLDKGIDLGNQVQNPDLPLMDNASNKLDIAPK
                     PDEPSKLDIIPKKDMSDTSIAKLEDTETTQVIHENVKKVTPVKNISKKNTKAKSNVKK
                     NKVVEVAKTDESHTKKVSQRSWSIQIASYNTLSKAQKEVKSLQTMKYDAYVDKADVSG
                     KTYYRVKIGPILKEDKAINMLQEIQSIDKYKESYIVKE"
     gene            complement(23482..25926)
                     /gene="gyrA"
                     /locus_tag="PLV81_03720"
     CDS             complement(23482..25926)
                     /gene="gyrA"
                     /locus_tag="PLV81_03720"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOM09244.1"
                     /translation="MTKKQDNPKNKRVHNIEIEESMKRSYLDYAMSVIVGRALPDVRD
                     GLKPVHRRILHAMNERAWRSDRPYVKSAKIVGEVIGNYHPHGDAAVYDTLVRMAQDFS
                     MRLPLIDGQGNFGSIDGDPPAAYRYTEARLSPIAQELLKDIDKDTVDFISNFDETRKE
                     PVVLPAAFPNLLVNGSSGIAVGMATNIPPHNLSEVIDAIVFMIDNPDATVKQLMKFVK
                     GPDFPTGGIIVASEEMIKAYAKGKGSIVVRARLHVEEQKNKSMIVVTELPYQVNKAAL
                     IAKIAELVNNKVIEGINELRDESDRNGLRIVIGCRKDAVTNIIINQLYKHTQLQTSFG
                     INLLALVNNQPKLLDLKGLITHYISHRKTVVTRRTQYELARAKERAHILEGLKIALDN
                     IDEVIAIIRGSKTVEEANERLIKRFKLSEIQSKAILDMRLQRLTSLEVKKVIDELREL
                     LKLIDELTAILKSEKRIFAIIKDELIAIKEKYHDTRRTEIIYEDTSVSFDAEDLIAEE
                     DMVITITNDGFIRRLSADTFKKQRRGGKGVIGLSSKKEDFITMMTIASTHDTLLLLSN
                     KGKIFGMKVYEIPVASKNTRGKSLKGIINLASGEMITAICSVNDFSENFLCMVTRNGV
                     MKKTALEEFSNVKKGGIIAINLHKDDELVDVKVVKSQDDVVIATRNGLLLRTNIAKMR
                     AMGRNSSGIIGMRVASGDQIIAMDVVKPGSDLCVVTSRGYGKKTQYSHFATKGRGGKG
                     MAYLKISEKNGYAVGVRSVHPEDEVIIASKKGMTIRLKAKEISYQGRTAAGVLLLQLD
                     KDDEVTDFAVLYEEKE"
     gene            complement(25980..27875)
                     /gene="gyrB"
                     /locus_tag="PLV81_03725"
     CDS             complement(25980..27875)
                     /gene="gyrB"
                     /locus_tag="PLV81_03725"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOM09245.1"
                     /translation="MTSYKADKIQVLEGLDAVRKRPAMYIGSTDINGLHHLVYEVVDN
                     SIDEALAGFCDTINVTINKDNSVEVIDNGRGIPVDIHPVYKTSALEIVLTKLHSGGKF
                     DNQAYKVSGGLHGVGISVVNALSEYMEVEVYRDGRRYFQSYKRGVPTDKVKDIGETKK
                     TGTRVLFKPDSQIFEETEFHFETLSKRMRELAFLNNGIKITLTDLRGKGKEHTFYYKG
                     GIVSFVQSLTENRTSITKKVIYLHDNRSNIDVEIALDYIDTYTETVFCYANNIHTKEG
                     GTHLIGFKSALTRVLNDFLKREGYDKKIPQLSGDDVREGLVAIISVKIPNPQFEGQTK
                     MKLGNSEVKGIVESITNEHLTQFFEENPQIVRKILEKCIDTARARIAAKKARDLTRRK
                     NALENDSLPGKLADCSERDPALCELYLVEGDSAGGSAKQGRDRKFQAILPLKGKILNV
                     EKSRLDKILSNDEIKTIITALGTGIGENEFDITKCRYHKIIIMTDADVDGSHIRTLLL
                     TFFFRYMPEIIANGYLYIAQPPLYNIKAGKEQYYAYSDEERDAIVKNLDKSKVTIQRY
                     KGLGEMNPEQLWETTMDPEKRTLLQVTLEDEVEADEVFSVLMGDAVEPRRKFIEENAH
                     MVENLDL"
     gene            complement(27872..28111)
                     /locus_tag="PLV81_03730"
     CDS             complement(27872..28111)
                     /locus_tag="PLV81_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09246.1"
                     /translation="MFIHIGERKSVSDKQLIAILNCETVVKSPEINSGFINKIGEEDK
                     TIAICTDSIITTKVSSYTVIKRYGQISDAVWSKKI"
     gene            complement(28092..28451)
                     /locus_tag="PLV81_03735"
     CDS             complement(28092..28451)
                     /locus_tag="PLV81_03735"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOM09247.1"
                     /translation="MKFRYKEKRYNRTIACSQILQDVASEIGLTEDLILEHVRAIWQK
                     AVGDIIATHSYPYRMINRVLYVVVDHPIYANEISLSQSLIIKRLSEISGSTAIDSLKC
                     TVKKKSYRKTNDVHSYR"
     gene            complement(28451..29548)
                     /locus_tag="PLV81_03740"
     CDS             complement(28451..29548)
                     /locus_tag="PLV81_03740"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOM09248.1"
                     /translation="MFIDRLLLKTFRNYVHETISFSPEINVISGANGSGKTNILEAIY
                     IASHIKSFRNCTDKDIIQWGKHEYYISVQVKNADISTIEIGTSVSDQQSGKKVKIDGK
                     EIKKLSDFYGKINVVAFFPDDTVIISGTPDVIRRYFDRLISKVDKEYIELLTSFRKIL
                     KNRNRLLKDILYKRDIIKQLDIWDSMYSDCASALVIKRKQYIEHFNTYFKTLYKELSG
                     FNDDLTIKYYASLHNENVTDVKNELSKKRNIDIQMGTTTLGPHRDVYVIYGNENTMFK
                     SYASTGQRRLASIAMKLSEVSLIYDIKKSKSIVLLDDVLSELDDDRRKNVLQKLIDGN
                     HQVIITCASSNDVNFVDNYKHLQVVHNRVSE"
     gene            complement(29564..30685)
                     /gene="dnaN"
                     /locus_tag="PLV81_03745"
     CDS             complement(29564..30685)
                     /gene="dnaN"
                     /locus_tag="PLV81_03745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOM09249.1"
                     /translation="MRIEVDKVQLVKATIIADSVVSAKSINTILTQCLFNVDGDTLTI
                     TGTDNEIGIKTQLDIVSDGNCSFTISGKRLVSLLKEFPDGVVILDVNNTNVSISSKSG
                     LVKGQYVLVGSSAEEYPAIPDFNFKNAIEIEQKTFSEMISKTVHAAAHDTVKPIFNGV
                     YIVSEDEKSMTVVATDSRRLSMIKKDITGSSTIKNGFILPLKTVNELLRLLEDKGTCL
                     IAFDDSRCFIKIGRTEIVSRLIDGQFPDYKQVIPKDYLLKAVIEVKKLKEALNRLKNF
                     TNEPSWRILLKFSQNTLLIEASYPDQGEGQEELAIDSDCKEPITIGINVQFLLDLLKE
                     INSFSVVICVTGVMSPLLIYPEDDIKFQSVIMPIQIRSM"
     gene            complement(30943..32286)
                     /gene="dnaA"
                     /locus_tag="PLV81_03750"
     CDS             complement(30943..32286)
                     /gene="dnaA"
                     /locus_tag="PLV81_03750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOM09250.1"
                     /translation="MTDSTVTIWNKVLGSVETYINKQSFNMWFKNTEPVAFNENILVV
                     KVPDDVAKRHISDHYASLIHSIIKEHTGKEIVCEFVTGNGFKEPVMNVPLPVTNDEKK
                     ETLQFPTILNPNYTFENFVIGPNNQLAHAAAISVSKAPATQYNPLFIYGGTGLGKTHL
                     MQAIGHRIIQERPYLNVLYVPCEQFINEFIQAIRANTLSSFKIKYRNVDILLIDDIQF
                     IEKKEQTQEEFFHTFNTLHNSKKQIVISSDRPPKELSTLEERLRTRFEWGMIVDIQLP
                     NLETREAILRNKAEKIKIELSDEVCNYIARRIKSSIRALEAALVRLSMVSSIYNQPIT
                     VQLAKDHCKDLFDVDSNKKITVQDIMVKVASKFGISVDDIISKNRQSKVVQPRFIAMY
                     LARRLTELTTIEIGKEFGDRDHSTVLNAINNIEKSIEEDIDFKEVIDELISELRS"
     gene            32613..32870
                     /locus_tag="PLV81_03755"
     CDS             32613..32870
                     /locus_tag="PLV81_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004423064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional coactivator p15/PC4 family
                     protein"
                     /protein_id="HOM09251.1"
                     /translation="MGVIIKDIKKNNNEIIRVEISEYQGKELINLRIWYQAIDNGEVV
                     YKPTQKGVALSVDKFSELQEAITKIAEYLHDKKHGTTPHNV"
     gene            complement(32851..34311)
                     /locus_tag="PLV81_03760"
     CDS             complement(32851..34311)
                     /locus_tag="PLV81_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09252.1"
                     /translation="MFYNTASHLLFYSLLYGLDNFVSREGVCEGIALSPWQAGKRIVK
                     KYYAEILLTKQSSKQYPVIITTDSEDIFKVIKDYIQQNISSIALRLSLLSKNNLQATF
                     AFNEPLDHTLYDSVHILFSAYIRCKTPHLVDDYFTIYMPLELFTIFRVKVSNYTPYNS
                     LNDIEAQFLQFFNDPYNLFPSLPIILETMEDNEFQKLIYFLLNEKILTPYHLYLLTRA
                     FPQHALKIKYNISSNLISDILHVGKTIHRITARDMIEGIYAFEEILYLKLRTKPYFVF
                     GNFIDQITNILHHIAIVSTFQKKTFETWLSEIEQSGLLYTILSHCDDITIATAFNDNE
                     KLFNQLSRYLSSRRINSIAVYLQNKYTYNHTILSQYTIVQLYLKNMSHINKLYAMPFN
                     QLLKKYIHPQMMYYILFDCGWFTIATALKQTPKKLVYDCIQKFPQGAQYCILDVYDGV
                     LNPNIVHDEMQIKKARQLVIQSLIKLHSNGTIHCEV"
     gene            complement(34329..35486)
                     /locus_tag="PLV81_03765"
     CDS             complement(34329..35486)
                     /locus_tag="PLV81_03765"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HOM09253.1"
                     /translation="MILQPFHPSGIPKNILVIDDSKLNRAIVKNTLSAYNIHVFEAAD
                     GMEGLNALKSKQFDLILVDIVMPNLDGFGFLEQFKQYIGNEFIPVILMTGLDDLNSKI
                     KGLTIGADDFLLKPLNQKELIARVQSLLRLKSAHDELYEKNQLIQKELEYAKRLQQFI
                     IPKDFSFVPYPSISGRYLPIADIGGDLFDCYKISDSKVGVLIADVTGHGIPAALVMSM
                     AKMTFSIYAYEILNTHELMSKINEEMRGLLLDMQYITAFYMLIDTEQQTISFTNAGHT
                     RPLYYRKSSDKIIALDTNGLFLGIQDESLYEQKTIHFEQGDRCILYTDGITEIKNDNK
                     EEFGEQRLAKFLKQHNEKPDDFCEHLLQTLDHFSPLHDRKDDVAFMFIDFS"
     gene            complement(35500..37680)
                     /locus_tag="PLV81_03770"
     CDS             complement(35500..37680)
                     /locus_tag="PLV81_03770"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOM09254.1"
                     /translation="MTTTIEKNYQIFIVSKDEDAVSAITKVFSDSLYTITLTKTGMEV
                     LSLISYKLPHIILIGTDITDINAFELVSLIKKNSITRYVPCIVFGYNNEIQDRISAID
                     SGADGYLPLPFKNEELYSLVHAKLIQHDELYLLSVTDDLTGLFTRKEFFRRFSFEIES
                     NIHSCVSIAIIDIDHFKNINDTYGHPIGDIVLIQLANILKSLQSDNYTPARFGGEEFV
                     VIFPGVQATEAKKIIDRIRLQFSSINFKTNIPDKTFSVSFSAGISEYPSMGTNLSELL
                     SRADQALYSAKTDGRNRVYTFSPIMSRNDKFWEYIKTNKIIKNIFIDPFLHEPVTGYH
                     YIPVVLENLLSSETAIDAIGVLIIKVLPLVDFYQYRGYKNFEYDIENISLLIPKICEI
                     HFPYERYVTVSDLYDYEIAILFPFPSSLKTNVSEFTKLCKELVLDISLQSIHHHVEIQ
                     YAHGVVPFHKHLARKILQEIKSIKSNFSPLLSLANEAEIISSFWQTIHSKKDISDYIF
                     LLPVYNTEFARSMCHVFSKQLVSAKNFFDIMIKNAITSQEHYIQFISSLANIVSAHKF
                     HLPFIINWVPSIDLSLQISTLQKVCTRFSLDKIVLAIDEIELHSFIPQYEGLSSKNTS
                     PVEFAISNCYIGSNLLQSLSTIEFSLVILSEHIIRNLHYFKDRIKIINGLRLFCDQIN
                     LPLYAPNILKDEEYRIIKDINIAYSSGPYIEQQYALFSASTKVK"
     gene            complement(37687..38529)
                     /gene="pyrF"
                     /locus_tag="PLV81_03775"
     CDS             complement(37687..38529)
                     /gene="pyrF"
                     /locus_tag="PLV81_03775"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOM09255.1"
                     /translation="MNYCESLIYNSRKHKSIVCMGLDPVLEDIPIRKDSIRNTIVTFY
                     EGILNKCLQHHIYPACVKPNYAFYAQYGIEGLHALCDVITLFGNEGLPVILDVKRGDI
                     GKTAMAYAKEAFEFFKADAVTLSPYLGFDSIQPFTSNYPDKGYYILVKTSNKSSSEIQ
                     DIVVNNEPLYLLIAKKLIEWYAPGIGAVVGATFPQQLNNIITVFEESGKDIPLLIPGI
                     GTQGGDMEAVIKILNNSKNKGLHRVNASSSINYAYKESQYHTKNFDEAAVEALKILNE
                     TLGY"
     gene            complement(38530..39066)
                     /locus_tag="PLV81_03780"
     CDS             complement(38530..39066)
                     /locus_tag="PLV81_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOM09256.1"
                     /translation="MAVDKRTILKHIHIQKGDITKMEVDAIVNAANPSLLGGGGVDGA
                     IHRAAGPQLLEECRTLGGAQPGEAKITKGYRLKAKYVIHTPGPVYRGGSHNEAAILRN
                     SYYNSMRLAKDNGCTSIAFPAISTGVYGYPKEEACSIALSTIIEFMEKENFLIDVYFV
                     LFDDDNYAIYTKHLQELS"
     gene            39204..41090
                     /gene="mnmG"
                     /locus_tag="PLV81_03785"
     CDS             39204..41090
                     /gene="mnmG"
                     /locus_tag="PLV81_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010679087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOM09257.1"
                     /translation="MIRYFDIIVIGAGHAGAEAALASARRGFATLLLTGNLDTICQMS
                     CNPAIGGLAKGHLVKEIDALGGEMGLAIDETGIHYKMLNKSKGPAVWAPRAQADKKQY
                     QLRMKEVLEKQKNLTVIQDIVDAIIVEGGQVQGVETIRGQQHVAKAVIVCTGTFLKGL
                     IHIGNYNEKAGRLGDFSAEKLSDSLRSLGFPVKRLKTGTPPRVNGNSIDFSKCEVQHP
                     DPVPVPFSYRTHSINRQQVPCYITYTTEKTHAIITNNLHLSPLYSGKIQGIGPRYCPS
                     IEDKVVRFSSRNRHQLFLEPEGLHTNEYYINGFSSSLPEDVQLEMIRTIPGLEEVQVM
                     RPAYAVEYDYVPPVELYHTLETKRVKGLYHAGQINGTSGYEEAAAQGIIAAINAGNKL
                     LGMPPLVLKRSEAYTGVLIDDLVTKGADEPYRMFTSRAEYRLLLRQDNADERLMRYGY
                     EIGLIDKELYEKTVEKYKRIQELTDRINTVSIVIDDKLQEILHNKQIAIEERSQIQAG
                     KLLRRPEISLDDVLPSIIQSDSSLVMNEQEAKIVEMNIKYEGYIKKDLERIKKLEQME
                     NKKIPETINYDEIIGLKNEAREKLKKIRPETIGQALRISGVDPSDISIILVHLESLHR
                     KQSG"
     gene            41852..42670
                     /gene="rsmG"
                     /locus_tag="PLV81_03790"
     CDS             41852..42670
                     /gene="rsmG"
                     /locus_tag="PLV81_03790"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOM09258.1"
                     /translation="MTISNTVIIPDEINRKLKEYARLIYEHNKTTNITGLKDTESIYN
                     ELILGSITPLKHVNVPRGTSFIDIGTGAGIPGIPLLLYFGGNIKGVLVDSNNKKIKFL
                     LNAINTLGINDIVTVIQGRVEEIAKEKAYRDSFDFAVARAFAHPLMALEYGLPFVKPG
                     GWLYIYYSFNLYEKGFENNKTVCSNDEVIAEYGCDKLHEEKNANKHDITVLTDYLTKH
                     SLNLGGHRVTLEEAKKLKLYEGLIFLKDGYTPANYPRKHAVVKREVESLRNIRS"
     gene            42676..43233
                     /locus_tag="PLV81_03795"
     CDS             42676..43233
                     /locus_tag="PLV81_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09259.1"
                     /translation="MKKKFLLCMLLVYFIPLTGINAQIPQGQLNSMIVKDETLIIPGI
                     GASSTVIDMSEQEVLLIKGNPFKKTQHVTHDFLKDVLKIQSEASLPFHYLYTYRNPLT
                     IIGFYNGRVSFVVVWDSYGVLIDGVRVSKGIMAIIFNYGNEGMQVLKDADGSIYMYSL
                     KGIAFIDEKNDDTVDGVIIFKSCSN"
     gene            complement(43240..44997)
                     /locus_tag="PLV81_03800"
     CDS             complement(43240..44997)
                     /locus_tag="PLV81_03800"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOM09260.1"
                     /translation="MQKKIDSYITHNIHYIDFCIGQLKKDIELLPNRTDAITEILEHI
                     LFQKKLTLPLSNEGITACNDLARVLTQWDIIQHGDLAGYIYQNIKDTDDKKKKGQYFT
                     PPAIVQYMVEHALQYHDNPFTVKILDPACGSGQFLIYIYQYLLELYKQHGISHEDAAY
                     TICQNNLFGYDIDPVACMIAQWNIQKLSGTHTNIFHRDMLSTDDLFNKHNSLVFDIII
                     GNPPWGAHFTHEQKQYFKQHYFSAISGINSFTLFIERSLSLLHERGILSFLIPEAYLN
                     IKAHQNSRQIVLSNTVIKEIVQWGDCFKGVYAPAISLTAQKEEREEIRKKHIVHIKHA
                     KSLTTIAHMIPQQMYCTTHESIFPINYTRKAVALLEHIHTLSYITLQDRVKFFLGIVT
                     GNNDYHISTIKDSRHPDPIIIGKDVEKFRINFSNHYFKYNVSTLQQVAEQQCYLTRNK
                     ILYKFIGRHLTFAVDPHGSYTLNNVNGFIPLSPQLDPDVLVAILNSRLLQYIYETQFF
                     TVKVLRGNLEKLPIKIINSTNKKLMKKLSLELREEADRVKRKILMDTIDDIVFHEYRI
                     NDMLAHHIHESITRGISIA"
     gene            45090..45440
                     /gene="hypA"
                     /locus_tag="PLV81_03805"
     CDS             45090..45440
                     /gene="hypA"
                     /locus_tag="PLV81_03805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOM09261.1"
                     /translation="MHELSIMSNVLDIVVQHASQNGASKVTKIHLLVGELSDYIPEWM
                     QTYFDFVSKDTIAEKAELVVEMVKASLKCDDCGNEFHFNKNDWQFSCPQCNSPNVTII
                     SGRELTVKSIEIEG"
     gene            45442..45930
                     /locus_tag="PLV81_03810"
     CDS             45442..45930
                     /locus_tag="PLV81_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009172365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HOM09262.1"
                     /translation="MISIAIVVVSDRSYRKEREDLSGKAIEEWARSRKYSVVEFCIVP
                     DEREVIAQTLRDLCAKNINCILTTGGTGFAPRDVTPEATADVIERYAPGFAEAMRHAS
                     LAITKHAMLSRAIAGIRGSSIIINLPGSPKAVVENLEVIQDALPHAVKLLQGQVKDCA
                     HE"
     gene            46135..47583
                     /locus_tag="PLV81_03815"
     CDS             46135..47583
                     /locus_tag="PLV81_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOM09263.1"
                     /translation="MKQQLTYAVVVVVTVLLYIPGVLVAGPIVSIERISVDLFPEITI
                     KGVIRNPEGIQISTIGRENFAIVEDNTHINDFTVNSLDTDTYIALCIDASKSISKKNF
                     LRLKKIAINIIKRSKGNYTFIVYKFNDEVVTLAPFSKDTTNYIEIIESLQQHGKRTKL
                     YNCLFAATEELARQKGGKSIILLTDGKDEGSFMFPDDVVNNAREHTIPIFTIVPERCS
                     KAAAVSRLSKITKGKLFVATAGVEEAVINVLQSRMLHTIPFEITFKTNKHDDAIHPIE
                     VQFKHKGIQDNDTGYAKYSFQKNTYTIHTDLPFVVLAGIVVLLVIALVIVILVLINKW
                     KQIVELVSMQRIPQPVERYYSMLYEKDDAVKKDTDTLLLATSPEYSYTNAWLVEKSGP
                     ETGKKFPLIWDEVTIGRDKENAIVINDDAVSLKHAKIKKIGNTYYLFDLASDNGTFLN
                     NKKLLRPKPLSDWDEIQIGRTSILFRGTKIKQ"
     gene            47589..49097
                     /locus_tag="PLV81_03820"
     CDS             47589..49097
                     /locus_tag="PLV81_03820"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOM09264.1"
                     /translation="MSLKVLLVSPPIFDFYYSFHRSEPLGLLYIKEALAHYDWLTVTI
                     FDARYKGTSKVITTPEVFNYLHHLYVKDTSWFSLFHGFKRFGYSYNTIVTYIKENEYD
                     VVCITSLFSAYHHDVEVLVERIKNETGAIVIVGGWAVWADTNILVNGRADFYIRGDGE
                     LTLPILLQEIYSSNGNISHLPKLIDSDNSSVNEFFMHTFPHRETWYTYYGKRIANIIC
                     SKGCVYHCDFCSIHSRYRYYQRTIDSIQQELEYLYAHGVKIVNFEDDNFLFNRKFAKQ
                     LLSLMKYYHNKGMYFLCMNGVTAPNLYAVFDDALDAGFLEFNLSLVSSYEEVVKQHNR
                     PALQEIIKDIAWKSAGNVRVIVYIIAGLPGATVKTCIDDILFLASLPVMIGFSPLYML
                     PGVRLFESMGVPDDRRLCRGSALYSFGEGFSREDIAALWKLCRFINRLKIADAINDEV
                     RVHYEFYKKACHDHVWYYRDEKGLWHEGFSFSASLPESFHICDINGRTRLIG"
     gene            complement(49099..49782)
                     /locus_tag="PLV81_03825"
     CDS             complement(49099..49782)
                     /locus_tag="PLV81_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008731394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOM09265.1"
                     /translation="MHKPPLTMQTTTLWNYPSQHYGNKQQGDQNYPGATPSYIIWNLL
                     MRYTKKGDLVIDPMCGSGTTIDVCNDTGRKCLGYDIHPYRTDIIKADARALPLENNKA
                     DFVFIDPPYGDNITYSDEKDCIGKLKATEKEYFTAMEQVIQEIYRILKHERYMGLYVC
                     DYYNKKDGFVPIGFHLFSLLTHYFTPIDIIAVVRHNRTLQMGNYHKAALEGNFLLRGF
                     NYLFIMYKK"
     gene            50147..50692
                     /locus_tag="PLV81_03830"
     CDS             50147..50692
                     /locus_tag="PLV81_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOM09266.1"
                     /translation="MDNKSSDCIAQCYILYGNYVKSIIARFYSDKDEIEDIFHDVFLK
                     LLSAGFCGDPHSHKTRNYIRKATRHVCISRMRKEIARQKYCNNSNPDSMIDIETVTDM
                     QTMDIGETVIDGMIVSTLYDVIDELEHDEQNLIFNKYFYNKKYFQLAAEQGVSYHVIK
                     RKLLAVNKKLKHKLMNTIAYQ"
     gene            50862..52958
                     /locus_tag="PLV81_03835"
     CDS             50862..52958
                     /locus_tag="PLV81_03835"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOM09267.1"
                     /translation="MVNVLKRFSYAVNRWFSFIALLTAGMISCLLVPFFIPNIEITEL
                     TRWYFALIIIINQAFFHYTQIFPRWEKKSPLFIIIISAIPGVITFLYTVATNSIILSA
                     SFDGLHIQFVYGQFIVLYLIVFIFYIMGTFLTLYYKVRRLENTSFRYQLFYVFMGNHI
                     GALIILISFIIMPYVFKRFEYHSLGISLAALMLLYINNYAISDERYLNFKEFYLKIVY
                     YGVVLVFTVIPTYWILVNSYRYFLAGGQIPAVAVAFINFVYLIFFTRIIQPYLEKWIK
                     REYAGLEKRFNEFIQALSQVEEFEGDPTYWDRFFTTTMDSLEYLFNVTSASFLMYNEP
                     EKAYILSHTIGQPPQVKRISADDSLVQLCQIAKTTIHQSLFYTDEIIKPFDDVLALVK
                     NNSIEVILPSFDQSGKLIGILFIGPHKKGRPYTFNEIEFMELYRVKFNSALVNSLQIE
                     EVKSKQIGEHDNLVVSAIKNSIIPRQMPYIDHIRISSFFMNNSNLGGEFFNAKVLHKN
                     ALAFLISDCSYSGVDAAVLALELYAAFESLSPTYDTPEKIMQLLNWVVCTSKFTERYA
                     TSFCFTFNSELLSLKYINAAFNPLIIYEIQNDSFIELDTKGIPIGIEKDFIYESKEYV
                     IKGPCIGCIYSNGITGAVNQTGQPYSIGRIKDLIRLHNNESPALIIRRISSDIDTYTS
                     NTAVKEDISIIIFRIE"
BASE COUNT        16884 a         9635 c         9074 g        17410 t
ORIGIN      
        1 aagaaggagg aatgataaaa aagtcaaaat agttgcggta agacgaggat ctgttcaaga
       61 agtaccaatg aacaggaagg ttaccgttcg tcatgttatg tggtcaaaaa gaccgggaag
      121 gagaatgagg aactgtaacc gcaacattca gtgacctgcg aggtcgaata ggagaatggc
      181 gcactaccga aaaggccctg ttcaaataaa atttaccaca aaataacggg ggtagacatg
      241 aggtatatag gcgtagattt acacaagaac agattcacag tttataatgc taccacagga
      301 cgataccgcg actatggtac caataaggta gggatagcaa gtttcataga ggaatgcaag
      361 cgtacggagg gtacgggagc gattgttgtg ggagtggaat cgacgggaaa cacacggtat
      421 tttaagaggc agctggaaga agaggggata caggtaaaag tgatcaacac gatgaagttc
      481 aaggtaataa ccgaatcggt aaagaaaaca gacaagcatg atgctcaaac gatagcagaa
      541 tttttagaaa aggatatgct tcctgaggca tggatatgtt cagactatag tgaaatgttg
      601 cggcgattat tacatatacg gagacaatta gtcaaggcga tagtaaccac gaagaatcaa
      661 gttcatagca tattgacagc gtatgggtat gaggatgcac gggcaacatt acaaagtaaa
      721 cgagggcgcc aggcagtgct ggacaccctc ggatcaacag aaatcggact cgtagtccac
      781 ctgttgatag aaatgataga ttcattagat gaaaaagtca agcagattga gggaaaaatt
      841 gaagaattgg taaaggggga taaggcaata gaactgttac aaacgatgcc ggggtgtggg
      901 cgcattacag cctggatgat acgagcatat acagatgata tcaggcggtt taggagtgca
      961 aaacactatg cggcttttgt aggggtggta ccctatgtgc ataattctaa tgaaaatatc
     1021 agacatggga agataaccaa acatgggcct gagctattac ggacagcatt tgtacaattg
     1081 gtaatgggta tgcggcggat aaaacaggtg caggattatc ggctaatgaa gcgctataaa
     1141 gcattacaaa gaacaaaagg ctcaggaaaa gcaatagtag caacggcacg aaagcttaca
     1201 cacatagtat ggcatatatt aacctataat gaaccatttg atgcttcatt gcaaatggta
     1261 tctggatatg agaataacag gtctatgcaa gaaaagcaat tagcataacc tgattattct
     1321 atagatttac aataatttga atagggctgt aaaatatttt gactttttat aggagactat
     1381 atgagcgact atattgcctt ttatgacctt gatcatacca tacttgcatc aagttcagga
     1441 acactcatcg tcagggacct ttacaaaagg ggtatggtat cattattatc tcttatcaat
     1501 ggtatgctac tttcactgct ctataaatct ggcattatca atacacaatc attgataatg
     1561 cattgggtta aacgattaaa aggaatggag gccaaaacca tagaacagta ttctgaaata
     1621 ttctttgaag agacagttaa acattacatt tatcctcagg ctcttgaatc aatatatatg
     1681 cataaagcta aaggagcatc cattgtatta ctttcggcat cactggattt tatatgtaaa
     1741 ccgataatga agcatcttca ctttgatgat gttttgtgta ctgaacttga agtaaacgat
     1801 ggttactata ccggttcaat aaaaggcaat tactgttatg ggatagaaaa acgtaaccgc
     1861 gctatgcagt tttgcacaga tcataacatc gatatcaaat ctgcctatta ctatgctgat
     1921 tctgttgctg atatacctgt tatggaagcg gttggatttc ctcaatgtgt taatccacag
     1981 cctgccttac tgaagctagc tcacgtaaaa ggatggcctg tccacaggtg gtgatttaat
     2041 gcttgaatct ctgaacaata ggtatacgcc ttccaacacc aaaagccttg gttgttacct
     2101 ttaagccaac tggtgcctgt tttcgtttgt attcatttct gtcgatagtt cgcagcacag
     2161 aatatactat gtcagggtca aatccatcag caataatctg ctctgcactc attttcagtt
     2221 ctacatatcg ttcaataatc tggtcaagta tctcataggg aggcaatgaa tcctgatctt
     2281 tctgattttc acgtaactct gcagatggtg ctttttccag aattgatctg ggtattatct
     2341 ctttatcttt attaatataa taggatagtg cataaaccag agtcttgggc acatcagaaa
     2401 gcaccgcaag gcctcctgcc atgtcgccat ataaggtaca gtatcccatg gataattccg
     2461 atttattccc cgtggtgagt aacagcatac caaatttgtt actgaatgcc atcaatatat
     2521 ttccacgtat tcgtgcctgt atattctctt ctgcaatatc aaatggcaag tctttaaaga
     2581 agggatttaa ttcatgtata taggtttcaa aaagattctt tattggaata gtatggatat
     2641 ttattcccaa attatgcgcc aattcaactg aatcatcaac acttcccttt gaagaaaatt
     2701 tagatggcat ggtaatcccc aacacatttt cgggtcctaa agctttaaca gccaggcatg
     2761 cagtcagtgc cgagtcaatt ccgccactta aacctaacac aaccttttta aatcctgatt
     2821 tatatacata atcccgtatg cccagaataa gtgctttctc aatgtcagca atgaaatcct
     2881 cttcacagct tatatgcata tattcttttt ctgtatcaaa tagtatgcaa tcttcctgaa
     2941 aatttttagc tttgagtaca aaattcccct ttttatcaat aacaaaactg ttgccatcaa
     3001 atataaggct atcattgcca ccaacctgat tgacatatat tattggcacc ttatattttt
     3061 ttgctacatg ctgaatttta ctccatttta tggaattttt cccctttaca tatggggaag
     3121 ctgatatatt tatcagtata tcagcccctt ttttcataca gttttcaatg gggtcaacag
     3181 catagcgcct gaactccata aacatatctt cactcagact catgtcattc catatatctt
     3241 cacatatggt gataccaata ttaattcctt tatagctaat gatttcctga gatggtgcag
     3301 gagaaaaata tcgtaattca tcaaatacat cataagtggg aagtaatgtt ttataatgtt
     3361 ttgattgtag tttcccatca tacataaagg ctgctgcatt atatagcata gggggattgt
     3421 cataattata atcaacatag ccacaaataa taccgcaatc actgcttaaa ggagctatcg
     3481 cctccaaagc cctcagatta tcatcaatga gttttttatg ctccagtaaa tccatcggcg
     3541 ggtacccaat tgttgccatc tcagggaaaa taactaaatc agcacccttt tcttttgcca
     3601 gagtaacaaa ctgagaaatc ttattacaat ttccttcaat atctgcaatg attggattaa
     3661 tctgagctat cgcaatcttc ataataatta cccttttcat gtgacctttt gtcaatatcc
     3721 atactaatat tgccgtgtat ttcttgtaat gatatgctga atagcgctgc ttaaaatcat
     3781 tttcaaaact atatcctgtt cctgagtgta aataataaac aatttttgta aagaacccat
     3841 atcacaaatt ttttttaacg agaatacaat tgctgttttt acatacattt cattttcttt
     3901 tgagtaactc tcaagcaacg gaatcactac atttttccct ttaaactgtg atagagccgc
     3961 tgctgcttca gcgcgtattc taaaatcttc atcatctaat gactctataa tcagcggtac
     4021 tgtgcgttta tcacggatga cccccaatgc atatactgca tacatgcgca aactcctgtt
     4081 ttcttcatta tgcacaatat gctgtaatcc cctgatatca cctgcatttt tagcagttat
     4141 cagtgctttt attatttttc gttttatata gacttcacgt tcagttaaca actgccgtga
     4201 taaatagata gtactttctt ttgatagtaa cgttgacaga gcatcaactg cagcatcccg
     4261 cacaagatca gagtaatctg ataatgcacg gacaaaaata taatatgctg ccctatcatg
     4321 caatgctgat aaaacattga ttgcctttac ccgaacttct tcattttcat cacctgaggc
     4381 aattgagcgt acatatggta acgcagcact gatttcatta tggacaacac aatcaagggc
     4441 aaaatatcgc actgatttat tggtgtcctg aagaagcctg acaataattt cgtaaaacct
     4501 gttgtcataa agatgatata gcgcttctaa tgcatatatt ctgtggataa cctcatcact
     4561 tctggctaaa cgtatgatat acgggtacat tctatcatcg ccattctttt ttagcgccag
     4621 tatgaccgat tctcgcacag caaaaaatgg tgacggtaaa aattcaatta tcaccggcaa
     4681 cactttttta tcatgcatct ctgcaagtat ttcaataacc atttgctgta ctgttattga
     4741 acgtactgaa tacaatatat ccttaaaaaa ttctatgttg gttcgtacct tacttttttt
     4801 cagtatctgt aatgcctgta ttttttgatc gttatctttc cctttgacta ttaactcttt
     4861 caatttcatg atgcattcat cagaaaggtt actatagcta tcatctacat tattaacaag
     4921 tgtcttaata cattcatcac ttgcaaaact aaccgtatac gcaataaaaa taaaaactac
     4981 tataatacct gtttttttca taaaacatga caccatcatt gtatagtcag gttacagtaa
     5041 aataatgcag atttataatc aataaaaaaa cccgacccca tgggccggga tatgaaggac
     5101 atactctatt aaaagtatta tttcaataca ccactgaaat aatgggtcac cagtgaatta
     5161 ataacttctt tatcagtaac atcctttttc tccttatcaa actggtatac caggaacatt
     5221 accgatccaa taagcataat tgcagtaatt tctgcattaa gtgttttctt gtagaggcct
     5281 tttttcttta tctcttctat atcacgtgaa acaatattaa tcaaatgttt taaaaactga
     5341 tcaacccgtt caatcacctc ttcatctata ccaacaatat ctttaaatat gagcttgatt
     5401 actattggct cacttaaaat agctttgata cattcagtta ttctatcagt attgaatttg
     5461 ataatatcct tctgtgacgg gttatccttt aaaagatccc tgagatcatc aggatcaaga
     5521 atatcatcaa tactctcttc aatagattcc agaattgcat aaaggatttc tcttttatta
     5581 ccaaaatact gatatactgt gccccgggca atattcaatt cttcacaaac ctgccctata
     5641 tgggtactct ggaaaccttt ctctataaac atctttttag caatatctac aatttgttgt
     5701 cggcgttttt gtccttttgc tggcatctgt ataccccttt acataaattc tattttattt
     5761 caccttttac acgaagcaat tcttctttga ttggttgcaa agcttcctga agttccttaa
     5821 tcattctttc agctttttcc tgcaattctt tactcatata ctatatattc ctttatatac
     5881 tataattata tatgcttact atacatatcg ttaacatttt cattatttta tataaaaaaa
     5941 gaatctttgc tataaacgtt atacatagca tacttttttg cgtcaacaac tttttataat
     6001 tttaatattg ttataattca acactttagt gtaataagaa cactttacaa ttgctttttt
     6061 tatctgtacc ccatataaca cagtcagctt cttttatttt atgattacca ttcattgtag
     6121 aagtattttc tttagctaat gttcaacaat tcatcaatat gacttcgtat ttcactatta
     6181 tcaggcttaa gggaaagggc ttttttcaga aatttttttg ccatattata ttcccctctt
     6241 ttataataaa catatccaag cgtatcgtta tatgctgcat tgttgggaac atgtgatact
     6301 gcttttttag ccagttccag agccctactc aggttcccat caggaagaag cgctataata
     6361 tacgctatag cattgcaggc attcatatta tcaggattat gttcaagtat gttttcatac
     6421 aactgcaaag cctggcgata gttacccttt ttctcaagtg caaatgcctt catctggata
     6481 atgtcatcat cctggtcatt ttgttgctgc aattcatcta aagccctatc ataatcccct
     6541 ttctgtatta atgtaaatat atacaccttg cgaacctggc gtatatcaat aaatgtcatt
     6601 ggcagtgaca tacaccgctt tagataatca atagcgctat tataatcctg caaatgaata
     6661 cagacaatag ccagattgta caatacaaca gggttttctg aatccattga ttcaagttta
     6721 tgcaaggtat ctttagcctg accaaaatcc tttttgtgat taagtagatg cgtagcctga
     6781 tcaagcaata ccttaatttg tttgtgtatg tctaattctt ccatatatga ttctttatct
     6841 ctcttttttt caaccgatct ataaaattat aatccgtaca atctatagtc aatggggtaa
     6901 ttacaatata tccattccgc aactcagtta catcgctccc tgttttttca tcattttcaa
     6961 ttgtaccatc caattccata tcaaaataat tattgtttct ctgtataatg cgatatgaat
     7021 cacgataaaa ccgtttccct aaacttgtat actgcacccc tttgattgca gatcgcggca
     7081 ggtcaggata atttatattc aaaaaaagtg gttctttact gtgaagtgga aaatccttta
     7141 acagttccag taaaaaccat gaggcatcgt aaaaataatc tgactcttca tagctgtcaa
     7201 ttgacaaagc tataccgctt acaccaaaaa tatatgctgt cctggcacct gcaacagttc
     7261 ctgaaaaata tatatcatcg cccaggttag gaccatgatt tacacctgat attacaagat
     7321 caaccggtgg gatgagcccg ccatgtaatc ctatattgac acaatcagca gtaaacccat
     7381 caacagcaaa ggtaaattca tcaatctgtt caacctttat tgatttgcgt atctgtattg
     7441 cattggaaca tccgctgcgt tctttgtcag gagctatcgc atataccgaa taattatccg
     7501 ataaaacttt ttttaaaacc tgtaatcctt tggcatgaat gccatcatca tttgttatca
     7561 gaatattcat aatactctct tacttaaata ttttctttac tgatacctta tctatgtgct
     7621 atataagaga atatatatca ccgtgcgtgt ataaaattat gccagtatac gaatataaat
     7681 gtaatacatg tcattatata tacagtgagc ttaaaaagat gggagatagt tactgtcccc
     7741 cctgtcctca gtgccactcg catgacacag aaaaaattat ttcaattgtc agtactattc
     7801 gtggtaaaca atcgtgctcg ggttgttctg ggaaaaactg cgcccattgt aaatagtact
     7861 tttaatttca aaaaaatgga ttacctatta ttacatgtat tagcgtaatg tattaactat
     7921 cctgaaatac tttttattaa tgatagtaag aatgatagca ccacaacaaa ctgtaaaggt
     7981 gattatagga attaatacat agattttacc ggtaaaactt atacaaaaaa agaaaagagc
     8041 aaaaaattct ttcttggtga aatatttcat gctgagagca aactttacca acacatcatc
     8101 ctgtggcaat ttttgtaaaa actctttatc gtatgcaacc agtgatcctg attcactgaa
     8161 tttgcgtaaa taccatacaa tcatcccaat aaaccagaac aatccaacaa aaagcaacgt
     8221 tattattatg ctggctgtac gtgctgaggt attctcaaaa tacagtactg atactgcaat
     8281 taaaaataaa agcagtgtgc cattatcccc aattgtatca agccatccac caatctttga
     8341 tactttaaat gtaaatttag caacctcacc atccacacca tcaaatactg atgcaagttg
     8401 aaacaaaagt cctcctaaaa ctacctgcca gtatgatccc tgtagcacaa aatatgcact
     8461 taaaaaaccc acaatcatat tgataatagt aagtacattg ggatgtatcc ttgttttggc
     8521 cagttgcaaa ctaattggaa tggaaattcg tttattaata ttttttgcaa tatatccacc
     8581 agtattatct atgatatatt tcacgaccag taattcggct ttctttatat cttcatcagt
     8641 ggaaattata aattggttat catttaaaac aggttcaaat acccccttct tttgtttaaa
     8701 gtaatcatca aaatgttcaa aatagtgagg ctgtacaaac aggtttgatg ggacatataa
     8761 agaccctttt tctggaactt cacccggtaa acactggtgt gcattaatct ttcttttttt
     8821 tatgtgtggc agaattttat gttcatacaa tattttttcc tgttcattca acataagata
     8881 tatttgttta atcccgactt tctgaagcgt ataaatcagt cgttcaagta tatataaacc
     8941 acagatcttt ttttcagtat gatatccatg aagactatga atatatgcct tttgtatcat
     9001 atcattaccc tttcaaagaa ttcttaaccc ggttttactg tgtcatcaac catacaataa
     9061 ttatctctta aaaccctttt ttatataatt tcgtcaagat gtttttacat aacattgata
     9121 taacatttta cttgacttta atacttcaga tattgtataa ttactattat catgtaagta
     9181 tatcatttaa atgttttttt ataaaggaga tattattatg aaatatgtaa atttcttttt
     9241 gtccattatt tttcttacta tatgcttaat actttactca gctccacagg cacaggaaaa
     9301 tccagagaag caatttaatc ctctctatga tgcagaagca gttatacatc gtttaagcta
     9361 tgataatttc aaaaatatta aattactcag gactgctata tataattttg gtggtggtga
     9421 acaggagttt aatagtttag tagatacata tgctgaagca agtgcactgt atttcagaaa
     9481 tgaaatgatt gcctcggcta atctcttcac aaaaaatgaa aaagatatca aagaagtagc
     9541 aatgagattg gcaaagcttt ataaagaaca ggctgaaaag cttcatattc aggttataaa
     9601 aatgggagtg cgccattctc ttaaagcatc aattgaacag acaaaaccca atccatctgt
     9661 tgatcctctt atcagcaatg caagttatgg aataaaaatt gcaaatgatt atctggtcag
     9721 gtcaaagcca atagatgcta tctattattt ccgaagagca aaggaaaatt gctttaaagt
     9781 ataccaggta ctgggtgaac agttaccgga agaatataag aaagatattg ttgataatca
     9841 gaataagata tatatagcaa aagaaaagaa aaattaattt gtaaattgaa attacatatc
     9901 tttcttacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg tgctcgttta
    10021 attggccttt tagaaagtat ttgcaaagct tcaattttta tagcattatg gggatcatgc
    10081 aaagcctgaa taataaggtt gtcagctctt tcccctttaa ttgttgcaac cgcctgtata
    10141 gcttctaacc gggttttcca gttatcactt ttgtatccat tctcatagta taccagaaca
    10201 gaatcatatc ctgtaatcgg tttaatggag ttacatgacc caattaaaac actgagcaac
    10261 catatcatta atccattaaa aatctttttt ccgcaggagc tggtcaatta atccttcctc
    10321 ctgtttaatc tttataatta tatttctctt ttcatctatt acataactat caaaccactt
    10381 gggtaacata taatcaggaa ctgtaatggg ttcttcaggg ggttgttgat aggcaactct
    10441 tgtttcttcc ctatctttta atatatatga ttttaatatt tttgtatccc tgtttgcaac
    10501 ttctacctca cctttaaaaa ccactacctt ggtttcaaat tgtgttgcta taataccaaa
    10561 atcagtacct cttaccccgg caattgcagt aggtgtataa acaattacac tggtgccttt
    10621 tattgtcttt gctgcaatta tccgcaattt ccctgtaacc atttgaatct ttgtaggctg
    10681 ttcggcttct tttaatctga tatcctgaac atacagcacc gatatctccc ggataaacag
    10741 tttgcttcca tcattcaata aaacagatac aaatgatttt cttccagtcc tgattttatc
    10801 tgtgtcttct agtatcgttc cctcattagg aattattcgt tttcctgaaa cagatgtaat
    10861 atcaacatct cccgataaat ccaggatttt cccaacgggc tttgctattg cattagcagt
    10921 aaaaaacagg atcaacacaa ttattacact tttcctgata cattgcttca taattgctct
    10981 cctccaagaa agaatattat tttaataagc atccttttat gagttccagg ataccttgat
    11041 gaatagccat tgagtttctc aaagtcacta ttcattgcaa aattttctac aagttcctat
    11101 caccaaccat catgctatat tactaccaca gacgttttac tacataatac ttcatacagt
    11161 actatgaaat gcaataattt actgtctgct taaatcgtat accagctgat attgatagta
    11221 aaacttttta atgcgctcaa tatagccctt agtctcagca aatggggcca taacagtgaa
    11281 catgtcttca ttatcataat gccatttcaa tacattgcca atacccgcat tataggcagc
    11341 tgaaactaat gtaatatcat ttttcagatt tttcattaac caggatatat aatatgttcc
    11401 taattctatt gacatacagg gattcttcaa catttcctta ttaacctgcc ccacccctgt
    11461 tttttttgct atttcttttg cagtaggcat catcaattgc atgaggccta tagcacctgc
    11521 tgatgattcg gcaaacggat tgaaaaggga ttcagctttc attaccgcat aaatgaaagg
    11581 ttcaggtata tcatattttt ttgaagactg tgatacgcat tgaacaaatg gcttaggcaa
    11641 aatgctttgg attatatcat tatgcattaa aaagatattt tctttcatat ctaaccgttt
    11701 taaaagctga actaaaccct gggctgacag aaaataattt ttgtacttta atccaaaaat
    11761 gtattgtagc acatatacat cattattatc aggattatta tatgctgcca gttccctgtt
    11821 tattccctca ctgtacccca ttgaaaagta atgttcaatc ctggtaacta tctcatatgg
    11881 aatatgtaat gacagcttct cattacgtgt aggaatagta gtatactgca tttcacggga
    11941 ttttggaaaa tattttttta tcatgtccag tcgttgtaag cgttttttat tatctcgctc
    12001 cttataggtt aacatggcat gggcaaaaag agtagctgta agattatttg tttgaagcgc
    12061 cattgaaaaa tcggaagaaa gcttttccac tgaatagttg ggtgatagtc gctcaagcaa
    12121 tatccatgta taagcagaat gaggcaaaaa cgctaccata gaataggcta ttgcttcagc
    12181 ttcatctgga ttttcctctt ttttcagatt ataaagccag aaacatacct catcagcgta
    12241 tttcccgttt gtaccaaatg tagaataata tttttcaaga taatataacg cagtttttgt
    12301 attggtaata gtatttttag aaaacaacca taataattcc tgtgtagcag tatcatcagg
    12361 atagtgtgct acatactcat ctacaggttt aggatctgtt tcttttctct ggacagcata
    12421 cagtgcttta taatacagta ccttctgttt aaattttgga ttatcagcat tttcaagttt
    12481 tgccagccac tgcatggcct gtcccttttt atcattatcc catagtgtat ccactatggt
    12541 agcagcaata tcatacctgt aatcagttgc ctgatatgca tcatatattg tttttaaggt
    12601 atcccatttc cccaaatggc ataatgttct aacacgtatc atatcaagat gtaatttttg
    12661 tgtgctttcg gtaaccagtt gtaaaaggtt ttcatctata gaaacaatat cctgataatt
    12721 ttttgtatta tacaaagctt caataataag ctgtatttca tcaggattca atgcaatatc
    12781 aggattttta gttgtaatct gtaatatccg ctttgctgca atttcagact gccagcttgc
    12841 tgtgcttatc tttaaaaggt tataataact attcactgca tcagtgtttt tgtcctgctt
    12901 ctcatacaga gatgctaatt ttatatacaa ccatgccagt gaatctctat cgttatattt
    12961 tgaaatactt ttctgtaata ccttttcagc cttgtcaaac tgtttcagtt gccctaacgc
    13021 ttcagcttta aggatctgtg catcacggaa tacacccgtt ttttcatgtt tcatcaaatc
    13081 aataaattta atgacatatt catattcctg atagtacatg aatccttttg caatttcata
    13141 cattgcatca ttatataaat atgatttcac agaaaaacgg ctgacaaatc tataaactgg
    13201 ctgcggatat atagatagtt ttcttgctct tcgttcaaca aaacagctat tggcaaagtg
    13261 atatagtgcc tttttataat ctttagcttc tttatacgct aatcccaaca tgaaatgttc
    13321 agtgtaggat agcccccctt tctgttctaa tgtttgaatt atagttttat tgttgctata
    13381 aaatcctaat tcagataatc cctgtggcaa tacaacgtca gaacaggaaa gaaaggaaaa
    13441 aatagctaag gtcaaaagag taaccatcaa tgtatatgtt cggataatca tgattattct
    13501 tcctctttct ggtaatctgc atactcgggc aattcttgca atgaaccatt actccacaca
    13561 tcatcataaa acgaataatt atcaataagc cgatagtagc tgtcagccag aacctttaac
    13621 atatagtttt tttcattttc atctttaact gattctaatt ttgttttcat atatgaataa
    13681 atatatggtt catttccaac gttctgttta ttttcaggtc tactatttga gtctttatga
    13741 ttaaatgcaa tatatgcaag taacgcatat cgtttagcct gtttggcttt tttcaaagcc
    13801 tgaatatatt tatacaaacg catcgagtac agtgttgtcc tgtaactatc ggccataacc
    13861 atatacatac gggactgttc attatttgta tagcctaaat tacaataatg ctttgataca
    13921 taatcatctt tcaagatagg tcctggcgac acggtatcta atagtttttt cccttcatca
    13981 atttcacgat gtaaaaccat tctgtataat gatatcagta attcttgagt tttacgaata
    14041 ttttcatacg aatctttaaa cttgaatttc atatataaaa attttgcaaa catatcatat
    14101 aatattgcct tgcggcagat tatcctttcc tcttccactg aaacatttga tacagaaaaa
    14161 ttaataaaat aaaagaagcg ctcattatct ttaagccctt tttctgcttt acgctgttct
    14221 ccagcactct gactgctctt tgtcctttgc gcaattagag tattttgaag tggtaaaagc
    14281 attaccacca ctatactaaa gataattatt gagattatag ctctcatgtc taaaaaccta
    14341 tgtctcatac ttagtattaa taaactcctt atattttatg attgactata tccatccgca
    14401 tactatagta cctgtataaa agtgttatac gtaatcatac tatcagggga tattattatg
    14461 aaaaccagaa caaccttatt attagtatcg attatttctt tactgtttta tataacctgt
    14521 actacatttg atccttcaat gttgcagaaa aaacccactg ttacttttaa agattttcaa
    14581 ttcaaagaaa taacgctgaa tgatatgacc atgctctttt cactggatgt tgaaaatcct
    14641 tatccagtaa cattgtactt agattccata caaagtcaaa tatatattga tacattctca
    14701 ttattttcac tacaatccgc agataaacta aaactatcca ggaattcaaa aacaaacaaa
    14761 acatttgcgg ttactatcac atacgaccaa cttataaagg ctgtaaaaga atattacaat
    14821 aaagattcaa ttgtcataac aataaagggt actattgctt tttcactgcc accatcaatt
    14881 cagattgtac aatcaatagc tgtccctttt acaattaaaa aggaaatccc aacaatcaag
    14941 ccaacaattg atatagcaca ttttaaatta attccacctt cactatctga tttaaaaaat
    15001 attataaaaa atatcaaccc acttgaaatt ccatctctgt acactgatat ccagaacttt
    15061 tgccagggga agggtgttcc tgatagtata tcaaaatttg atatacctct atctgttgca
    15121 tttgatatta taatcaaaaa taaaacaaag gccgcattgg tcggcaaatc gctcaattat
    15181 aattttatac tccacgatag tgaaatagct aaaggtcaac caaccataca gaatgctgat
    15241 ggcgcttcaa aaatcaactg tgtcactact ttaagcacca aaaatatcag caaaggcctg
    15301 acacaggcta ttgcacagaa gaatattcag tacacaatga aagggaatat gctcttcaat
    15361 gctgcattaa aagggacaga attacctgta ccatttacaa ttaatcagca gggcaccatc
    15421 aaatggtaag aatatacaat ttaataattg actcatacat actgtaagaa actatagcaa
    15481 cacagttatt atttatacat catgattaaa aggagttcac aatgaacgtt attcctgtaa
    15541 tacttgctgg tggttcaggt acaaggctat ggccacttag caatgaagaa aaacccaagc
    15601 aatttcataa tattacaggc gaaggcacgc tgcttgaact gacaattaac agactaaaac
    15661 cacttaatcc tgatttttgc cttattgtca cagcacgtat gtatgaagaa atgtcctatg
    15721 atgaattaaa aaaaacaggg ctaaagggga gtattatctc tgaacccatg ccaagaaata
    15781 ccgcgcctgc aattctgtat gcagcaacct atctttcaaa attatatgat gatgccataa
    15841 tgcttatttt gcctgctgat cattatatca aaaaaacaga tgtatttgta actattctga
    15901 aaaaagcaat tgaacacgca cataataatc atctggtaac tatcggtatc accccaacat
    15961 atccagaaac agggtacgga tatataaaag cctatgatga cccttcagaa gtaaaacccg
    16021 ttgagatgtt tgttgaaaaa ccaaatttag aaacagcaaa aaaatacctc agggaaggta
    16081 attacttctg gaatagtggt atttttgtat ggaaaatatc cgttattctg gaatactatc
    16141 aaaaacttat gccaaaacta taccaggcat ttcagcctct gcgcgatctc accgtagaac
    16201 agcttaccca gaatgatgaa ttgccatggg aaatcaagcg taatgtattc tcacagattg
    16261 aagcaatatc cattgactat ggcatcatgg aaaaagctga ccagcggtat gtaattcccg
    16321 gtgaatttgg atgggctgat ttaggtagct gggcttcaat tgatgatgta ctatcgccca
    16381 atgccgatat gaatcgatct ccctatcctg aaaaggttat ttttcacctt tcaaaaaatt
    16441 gcaccgtatt ccctgatact aaactcattg cagtggttgg cttgcatgac gttgttgtgg
    16501 tagattcagg aaatgaaatc cttgttatgg ataaaaatgc atcccaggat ctcagggaag
    16561 ttgtgaatcg tatcaagaat aatcgttaaa aacggcacta atagcgtttt tgattttacc
    16621 cctgactcct gcattaagag aagagctttg attgagcgat agctctcgca tatgcaggtg
    16681 gtattcaggc tcactgtagt aaaatacagg cactggcacc gtattaaaaa aatcagctac
    16741 catcatcaat gatgtgttaa ctaaagttac cacaatacta tccggagtac ttttttcagg
    16801 aactatcggt tcccctaact tatcttcaat caattgcaca atccatataa agctgagcgg
    16861 atcaatgatg ccaattgaat aagtatacca gttatccgta tatctaacca gtatcttttt
    16921 taagcgatca tcaagtaatg tatatgcagg acaaaagtaa tcattacata tttctttccc
    16981 aaatagactg caatctcttc tgtcaatccc attgttaact gcagggtgca tcagacatcc
    17041 tggagtattg ctgtcaataa accccatata gggacacaca taggttgtgg tttccctgaa
    17101 cgtatactgt ttttgcagca attcaaaatt ccttacaatg ttgccagtta ccagtgagta
    17161 gcgtatttca gcagttgcgt cagataaaaa gtgctctaac cctgcaaaag agatatcagc
    17221 acagttaaaa agcccacaac acacacaaca cccttttcct tgtgcaggct ggcataggga
    17281 tattttattg gaagcattca tattaattca cttgcacatt cccatgcatt ttggtatgtt
    17341 aatattaaac gtacaccaca tttatagtat agcagatttt tttatgaaat atagggataa
    17401 tctttacgag cttcttgatg tttccccact ctcttcaaag gaggagatac ggagggcttt
    17461 ctgtacaaag gttaaagcgt tacatcctga cagcacaggt ttaagctctg agagatttac
    17521 cgtagaactt cagaaggtca ttgaagctta ccgcatcctc atggatgaca gagagcgtgc
    17581 ccattatgat gccacactct tacaatatcc taaaaaactt gagaagcact atataccaca
    17641 aaaacgcatc agatactcca tatcacttaa agatttatta aagaaggagt ttctaccaaa
    17701 aaagatgcgt cgcaaggaca ttattcatga atttgaccac gacattgaga tagtatgcac
    17761 acaacaggaa atttcaaaag gggcagtagc attagttcag ttgccatccc gtatactgtg
    17821 tcctctttgt aacggtcagc accgtgattg ttacgtatgc aatgggttgg gacgtatatc
    17881 cagtgcttca acattcgaga ttgaactccc tgctgacata acaaaaacaa caatattaac
    17941 tgttgacctc agaaaataca ggccaggtac ttttaccgat ctaaccataa agtacctgcg
    18001 tataaaagtc accatagtat aaaggtagcc tacctgaatc gtattctcaa cgaatcacca
    18061 tgtttatcat caaatccttc aacctgcgac atacgtgata catgttctgt agcctgctgt
    18121 agtgctgatg gtgtcagata ctgaaatgat gtacggcgca taaacgtttc aacaccaagc
    18181 cccgaagaaa agcgagcagc tcctcctgta ggcagtacat gattggtccc tgaaaaataa
    18241 tcaccaactg caacaggagc ataagccccc agaaagagtg atccaacatt tttgatttgt
    18301 gtaagatagt gaagtggatt ttccaccatc aattccatat gctcagggcc gtaattattg
    18361 gaaaaatata tggcttcctc aattgatgag acaagaataa tatgagcatt ttgtattgcc
    18421 tgttttttaa tttcatgcct gcctcttccg gagtttatat cttcttcaat atatgtgatc
    18481 actttttgtg ccaacgattc agatgttgtc acaagaattg caatggccat ctcttcatgc
    18541 tctgcctgtg acagtaaatc ccatgctacc cagtgagggt ttgcttgctc atcggctatt
    18601 atcaacacct cacttggtcc ggcaagcgaa tcaatctgaa tgactccctg gctgaagaga
    18661 tatgccttgg cagcagttac atagatattg ccgggtccca caataatatc ggctttgggc
    18721 acagtctgtg ttccaaacgc tgcagcagca ataccctgtg caccgccaga tttgattata
    18781 gttgtgatac ccagtatgtg tgccattgcc ccaatatatg aagctattgc cccgtccttt
    18841 gaaggtggtg ttataattga aatatgtttg catccagcaa tctgggctgg aataactccc
    18901 atcaatactg aagagggata tgacgctttc ccaccaggga catataccgc tgcactttct
    18961 actggttgat acagcatacc aagagtagta ccatcttccc gtgtgtagat gaacgaatcc
    19021 cttttctggt gcagatggaa ttcgcgtatg ttttctattg cccttgtaaa tgaatcaatt
    19081 actttggcat cagtattatg atatgctgct gtaatttctt tctctgttgc acacacttcg
    19141 tgtaacgtaa ccccgtcaaa cttattagta aattctataa cagccttgtc accttcatca
    19201 cgtactttct gcacaattgg aataacgtta ttaaccatta ttccggtaaa atcatcacca
    19261 aaacgctgaa acagtgtatt gcgttctttt tgcgtcaaat cagataattt cctgatagct
    19321 atcatggttg ctcctcatta ttatagaagg ttattcatta tttcttgact ttatcattac
    19381 cattctttaa aatatcacaa actgtacagt aaaaaaatat ttctggaggt cctatgttca
    19441 agcatatagc agtaataatg atagcgatag ctcttgttat aaccggttgc aaaaaaacca
    19501 ccataacacc aaaacttttt gtcaccatac aaaatgaaat tcttgacagc gatatgcagc
    19561 agacaacaaa agaagacatc gttaaaaaat atggcattac tcttcaacag tatgaagaat
    19621 atgaaaaaca ggttgaaagt gatcctgagt taaaagcact ggttggaaaa gaacgcctca
    19681 acatcatgaa aaaatagcat cacgctattc caagaactgt ttccacattc ttatataaga
    19741 catactctgc actttctcca agtgcttcta tatgttgttt atagcgtgaa tactctagta
    19801 gtgggaaatc actcccaaat agtatttttt cttttccaca catctgtatg gctatgtcat
    19861 aaataccaga atcatacaga aaaggagttg ctgcagtatc ataatacaca tgagtcaagg
    19921 atttcttaac ctcaggcatc aactcataaa ataataggcc ccctccccag tgtgaacaga
    19981 taattggtat gttagtatac tgcgaacaca gtgcaaaaag actgcgcata tctgtataat
    20041 attttccagc atagatatgg cccaccggtt cattgacatg caggcataca ggcaaagtaa
    20101 acttttcagc tatagcaaga atctgtccca gtaacatttc ctcttcttca ttaaatccat
    20161 tagcataaaa ggctacttca ccaataccat ataatcccag ctttttaatt gattctatct
    20221 gtttgtacac atcaccgttc agagctatcg ccccaaatgc atatattttc cctttatatg
    20281 tatgctgcac agtagcaaga tactcattgt gcatttgagc atactcttcc ttttgccagg
    20341 taaaacccag caccagcgag gcatccacac cattgtgttc cattgattct acaagcattt
    20401 cagcatgtgc aatgcgtgct ttgccataat atagcagaga aaattgtctg tcattgcaat
    20461 atcgttcttt gttttgtacc atatcattag taaaaatatg tgtatgccca tcaataatca
    20521 tcgtataccc tattatgtat tgacattatc atatactttt ttagtatttt atcttcaggc
    20581 aacgtttact actatttatt atgaatacaa tagaattttt tttagaaccc agcaaagaaa
    20641 actaccatac acttcgtgtt aaggcggaag atggtagttt tcgctatatt cacagcaaat
    20701 actacccaac aaaagaatca tcatacctgc aggatattgt caaccctgaa aaatacgatg
    20761 cctgtattat tttaggcatt ggccttggat accatctgaa atatttggcc actatcatac
    20821 ctttttttaa acagattatc tgcattgata ttaataacta tctaaccaat gccaatattc
    20881 cacaggatat acaggcaatt attcatcacc cttcggtaca attggtattt gtgaatcccg
    20941 ataatcttca acagttacgt gatactattc agttacaatt ttccaaaggt atacaggtca
    21001 ttgaccaccc tgcttccttt catgctttcc cgcacatttt ttcatccata aaaaaaatca
    21061 tacagcagat agtagacaaa tcagtatcaa atcatcttac atcatcattg tttgcatacc
    21121 ggtactttaa aaatgcatta ttaaacctga accaattacc tgaatgcttt gctgtaacac
    21181 aatgtaaagg aattcatgcc catcagccct gcatcatcgt agcccctggc ccatctctgg
    21241 atgaaacact ctatcacctg aaaaaacacc acaattgcat catcatatca gttgattctg
    21301 cactcaaagc attatatgat cattctatca tacccgacta tatcgtttcc attgaccctc
    21361 agccagttgt gtttgcacat atttttccta cacaacccac ggtgccagta atcacaacac
    21421 ttaccgcaca cccaatgact ttttcattgc cagcaattgt atcattgaac acacaccccg
    21481 tatgccagtt actggaagag ttgtaccccg ggctaattgg ttctgtggac agtagaactg
    21541 gaaccgtact gggcgatgca gtcacatttg ctatctttac tggctgtaat ccaatagcca
    21601 ttgcaggtgc agatttttca tttcccaagt gtgttacata cgcccgatca acagaatacc
    21661 attaccgcta tcaacttatg cataaccgtt atacaccact tgaaacaaaa aacatggact
    21721 acataatcca tgcgtcaaaa cgtacaagaa ccacaacctt ttcaaggaaa aactttatcc
    21781 agtatcatga tgcagttgat gctatccttc ccaaagacac atcactattt catatcaatc
    21841 ccgtattacc acttgcccac gcaaagtcaa tagcaatcca agacttcctt tcactgccac
    21901 aggtcagcca ctatacaaaa aagatgacag cacgtttgct tcaacctcta tcacattcaa
    21961 taaatctcaa tgaaatatat actattctta ataatgaaac aatgtttaat actattgtaa
    22021 actcctccca agtacataat caaaaaagca ttcatagaat tcattattta ttacacaagg
    22081 agtaaatcta tgaggattgg cgttacaggc atttatgcat ctggcaaggg aacagtatgt
    22141 tccatgtttg aaaagttagg ggcgatagta attgataccg acatcattgc ccgtgaaata
    22201 gttaacccgc caagcccggt gcttgataca ctggtgcata catttgggca tgatatactc
    22261 aatacagatg gaacactgaa cagaagatat ttagcgcaaa aagtattcaa tgataaaatg
    22321 cttgttgaga agcttaattc cataacccat cccgcaatat tgcaggaagt tatgcaaaga
    22381 tcccatgata gtagcaaaat ctatatgatt aatactccac tgctttttga aacagaatat
    22441 cacaaacata tggataaaaa tatcgtggtt gttgccaata cagagcaggt gctcaagcgt
    22501 ggagtaatcc gtgatggcat aactgaagat gaaatcaaag cacgattaaa tcatcaaatt
    22561 tcacttaaag aaaagatgaa actggccgat tatatcatag ataattctgg atcattagaa
    22621 aatactaaaa agcaggttga agaattatgg aaaactttga ttttaatgaa aggccagtag
    22681 ccagggaaaa aaatgttttt ctccttcatc ttgatgttcc ccgcattatt ctgatcagtg
    22741 ctgtacttat tggcataata gttactacat ttcttttagg aatgaatttc agtgaaaata
    22801 aggttgtttc ccataataca ttagttcctc ctgaaattcc aggggacacg atagcacagc
    22861 ttgataaagg tattgatttg ggtaaccaag tgcaaaatcc tgatctgcct ttaatggaca
    22921 atgccagtaa taaacttgat atagccccta aacctgatga accatcaaaa ctggatatta
    22981 ttcccaaaaa agatatgtcg gacacttcta tagcaaaact ggaagatact gaaaccaccc
    23041 aggttatcca tgaaaacgtg aaaaaagtaa caccagttaa aaatatatca aaaaagaata
    23101 ccaaagccaa aagcaatgtc aaaaagaata aagttgttga agtagcaaaa actgatgaat
    23161 ctcataccaa gaaagtatcc cagcgatcat ggtcaataca gatagcatcg tataatactc
    23221 tttccaaagc ccagaaagaa gtaaagagtt tacagacaat gaaatacgat gcatatgttg
    23281 ataaagcaga tgtctctggt aaaacatact atcgcgttaa aataggacct attctgaaag
    23341 aagataaagc tataaatatg ctgcaggaaa ttcagtcaat tgataagtac aaagaaagtt
    23401 acattgtaaa agaataatag caaaaaaccc gcttgtacat agcgggtttt ttatactatg
    23461 ttttgttttt acttattttt tttattcctt ttcttcatag agaaccgcaa aatcagttac
    23521 ttcatcatcc ttatccaatt gcaataaaag aacacctgct gcagttcttc cctgatatga
    23581 tatttctttt gcttttaatc gtatggtcat ccctttttta gatgcaatga taacctcatc
    23641 ttcaggatgc actgaccgta ccccaaccgc atacccattc ttttcactta tcttcaggta
    23701 tgccattcct ttcccgcctc taccttttgt ggcaaaatgt gaatactgtg tcttcttacc
    23761 ataccctcga gaggttacca cacaaaggtc tgatcctggt ttaacaacat ccatggcaat
    23821 aatctggtca cctgatgcaa cacgcatacc aataataccc gaggaattcc tgcccattgc
    23881 ccgcatcttt gcaatatttg ttctcagcag aaggccattt ctggttgcta tcaccacatc
    23941 atcctgcgat ttaaccacct taacatcaac aagctcatca tccttatgca gattaatagc
    24001 aatgataccg ccttttttca cattgctgaa ttcttccagt gccgtctttt tcataacacc
    24061 attgcgagtg accatacata aaaagttttc actgaaatca tttacggaac aaattgcagt
    24121 tatcatttcg cccgaagcaa ggtttataat gccttttaat gacttacccc gtgtattttt
    24181 tgaagctacc ggtatctcat aaaccttcat gccaaagatt ttacctttgt tagatagcaa
    24241 taacaatgta tcatgtgttg aggcgatagt catcatggta ataaaatctt cttttttaga
    24301 agaaagacca atcacacctt taccgccacg acgttgcttt ttaaatgtat ctgccgatag
    24361 tcgccgtata aacccgtcat tggtgatggt gatgaccata tcctcttcag caataaggtc
    24421 ttctgcatca aagcttactg aggtatcttc gtagataatc tcagttcttc ttgtatcatg
    24481 gtatttctct ttgatggcaa taagttcatc tttaattatt gcaaaaattc ttttttcgct
    24541 ttttaatata gctgtcagct catcaatcaa ttttaataat tctctaagtt catcaatgac
    24601 cttttttact tccaggcttg tcaatcgctg caaccgcatg tcaagaattg cctttgattg
    24661 aatttctgat agcttaaacc gtttgatgag acgttcattt gcttcttcaa cggtttttga
    24721 ccctctgatt attgcaatga cttcatcaat gttatcaagt gctatcttca atccttcaag
    24781 tatatgggct ctttcttttg ctctggctaa ttcatactgt gtacggcggg taacaacagt
    24841 ttttctgtgg cttatgtaat gtgttatcaa tccttttaaa tccagaagtt ttggctgatt
    24901 gtttaccagc gccagaagat taatgccaaa tgatgtctga agttgtgtat gtttatacag
    24961 ctgattgata ataatattgg taacggcgtc ctttctgcag ccaatgacaa ttcgcaaacc
    25021 attacggtct gattcatcac gcagctcatt aatgccttca ataactttat tgttaacaag
    25081 ctcagcaatt tttgcaatca atgcagcttt gtttacctga tacggcaatt cagttaccac
    25141 aatcattgat ttgtttttct gttcctcaac atgaagcctg gctctgacca caatacttcc
    25201 tttgcctttt gcatatgctt ttatcatctc ttctgaagca acaataatac cacctgttgg
    25261 gaaatcaggg ccttttacaa atttcatcaa ttgcttaacg gtagcatcag ggttatcaat
    25321 catgaataca atagcatcaa taacttctga taaattatgg ggtgggatgt ttgtggccat
    25381 cccaacagct atacctgagg aaccatttac cagcaggttt gggaacgctg caggcaatac
    25441 taccggttcc ttgcgtgttt catcaaagtt tgagataaaa tctacagtat ctttatcaat
    25501 atctttaagc agttcctgtg caatgggaga tagtcgtgcc tcagtataac gatatgcagc
    25561 aggtgggtcg ccatcaattg aaccaaagtt accctgtcca tcaattaaag gtaaccgcat
    25621 agaaaaatcc tgtgccattc taaccagggt atcatacaca gccgcatcac catgggggtg
    25681 atagttacca attacttcac caactatctt ggctgactta acataaggcc tgtcactgcg
    25741 ccatgcccgc tcattcatag catgaagtat tctacgatga acaggcttca atccatccct
    25801 gacatcagga agtgctcttc ctacaataac gctcattgca taatccagat atgagcgttt
    25861 cattgattct tcaatttcaa tattatgaac tcgtttgttc ttaggattat cctgtttttt
    25921 tgtcacagtc tgttaccttt attcaaagaa taaaattata tagttatagg aaaatatttt
    25981 tataaatcta aattctcaac catatgggca ttctcttcta taaattttct ccgtggctca
    26041 acagcatcgc ccattaaaac agaaaagacc tcatctgctt ctacctcatc ttccaatgtg
    26101 acctgcaata atgttctttt ttctggatcc atggttgttt cccatagctg ttcaggattc
    26161 atctccccta aacctttgta ccgctgaatc gtcactttgg acttatctaa attcttaact
    26221 atcgcatctc tttcttcatc actgtatgca taatactgtt ctttaccagc ttttatatta
    26281 tacagtgggg gttgggcaat atataaatat ccattggcaa taatctcagg catatacctg
    26341 aaaaagaacg ttaatagcaa tgttcgtata tgagaaccat caacgtcagc atctgtcatg
    26401 ataataattt tgtggtacct gcactttgtg atatcaaatt cattttcacc gattccggta
    26461 cccagtgcag taataatggt cttgatttca tcattggaaa gaatcttgtc cagacgggac
    26521 ttttcaacat taagtatctt tccttttaag ggcaaaattg cctgaaattt tctatctctt
    26581 ccctgttttg ctgaaccacc tgcagaatca ccctcaacaa gatacagctc acatagtgcc
    26641 ggatcacgtt cactgcagtc agcaagtttc cccggtaggg aatcattttc taaagcattt
    26701 tttctacggg ttaaatcacg tgctttcttt gctgctatgc gcgcacgcgc agtatcgata
    26761 catttttcta aaattttgcg tacaatctgg gggttttctt caaaaaactg ggtaagatgc
    26821 tcattggtta tagattcaac aattccttta acttcactgt ttcccagttt catttttgtc
    26881 tgcccctcaa actgagggtt gggtatctta acgcttataa tagctactaa accttctctg
    26941 acatcatcac ctgatagctg gggaattttt ttatcatatc cttcccgttt taaaaaatca
    27001 tttaatacgc gggttaatgc agatttaaat ccaatgagat gggttccacc ttcttttgtg
    27061 tgaatgttat ttgcatagca aaatactgtc tcagtatatg tatcaatata atccagggct
    27121 atttcaacat ctatattact ccgattgtcg tgcaggtaaa taaccttttt tgttatagag
    27181 gttctgtttt cagtcaagct ttgaacaaaa gaaacaatcc cacctttata gtaaaaggta
    27241 tgttcttttc cttttccacg caaatcagtt aatgtaattt taatgccatt gttaagaaat
    27301 gccagctccc tcatcctctt tgaaagagtt tcaaaatgaa actctgtttc ttcaaatatc
    27361 tggctatcag gtttaaaaag tacccgtgtt cccgttttct ttgtttcgcc aatatcctta
    27421 actttatctg ttggcacccc tcgcttatag ctttgaaaat aacgcctacc atccctgtac
    27481 acttctactt ccatatattc agacagtgca ttcacaacag atattccaac accatgtaaa
    27541 ccaccggaca ccttatatgc ctgattgtca aatttgccac ctgaatgtaa tttggtaaga
    27601 actatctcca atgccgatgt tttgtacacg ggatggatat caactggtat acctcggcca
    27661 ttatctatta cttccactga attatcttta ttgatagtta catttatagt atcgcaaaat
    27721 ccggccaaag cttcatcaat tgagttatca acaacttcat ataccagatg atgaagacca
    27781 ttgatatccg ttgaacctat atacattgct ggacgctttc gtactgcatc cagtccttcc
    27841 aatacctgaa ttttatctgc tttgtaactt gtcatatctt cttactccac actgcatcac
    27901 ttatttgccc ataccgctta ataacagtat atgaacttac ttttgtcgtt ataatgctat
    27961 ctgtacaaat agctattgtt ttatcttctt ccccaatttt attaataaaa cctgaattaa
    28021 tttcagggga ctttactact gtttcacaat ttaaaattgc aataagttgc ttatccgaaa
    28081 cactcttcct ttcaccgata tgaatgaaca tcgtttgttt tcctgtatga tttcttttta
    28141 acagtacatt tcagtgaatc aatagctgtt gaacctgaaa tttcagataa cctcttaata
    28201 atcagggact gtgataaaga aatctcattt gcatatatag ggtgatccac aacaacatat
    28261 aacacacggt taatcatcct ataaggatag ctgtgagttg caatgatatc cccaactgcc
    28321 ttttgccata tagcccgtac atgctccaga atcaaatctt cagttaatcc aatctccgat
    28381 gcaacatctt gtagtatctg gctgcatgca atagttcgat tatatctttt ttctttatat
    28441 ctaaacttca ttattctgac actctattat gaactacctg taaatgttta tagttatcca
    28501 caaaattcac atcattacta cttgcgcagg ttattataac ctggtggttg ccatctataa
    28561 gtttttgcaa aacattcttt cttctatcat catccagttc agacaataca tcatcaagca
    28621 gaacgataga tttacttttt ttaatatcat atattaaact aacctcagat aatttcattg
    28681 caattgaagc caaccgtctc tggcccgttg atgcatagga tttaaacatt gtattttcat
    28741 ttccatatat aacatacaca tccctgtgtg gcccaagagt tgtggttccc atttgtatgt
    28801 caatatttct ttttttgctt agttcgtttt tcacatctgt tacattttca ttatgcagtg
    28861 aagcataata tttaatagtt aaatcatcat taaatcccga aagttcctta tatagcgttt
    28921 taaaatatgt attaaaatgc tcaatatact gtttccgctt gattaccagt gcacttgcac
    28981 aatcagaata cattgaatcc cagatgtcaa gctgttttat tatgtctctt ttatacagta
    29041 tatctttcag taatctatta cgatttttta gaatttttct gaatgatgta agtaactcaa
    29101 tatattcctt atctaccttt gaaatcagcc tgtcaaaata tcttctaata acatcaggtg
    29161 tcccacttat tattacagta tcatcaggga aaaaagctac tacgttaatt ttaccataaa
    29221 aatcagataa tttctttatt tcttttccat ctatttttac tttttttcca gattgttgat
    29281 cactaactga tgttcctatt tcgattgttg aaatatcagc atttttcacc tgtactgata
    29341 tatagtattc atgttttccc cactgaatga tatctttatc ggtgcaattt ctgaatgatt
    29401 ttatatgaga ggctatatag atcgcttcaa gtatatttgt tttaccagaa ccatttgctc
    29461 cagaaataac attaatttcc ggtgaaaaac tgatagtctc gtgaacataa ttgcgaaacg
    29521 tcttcagcaa caatctatct ataaacatta ttttttattt acgttacatt gagcgtatct
    29581 gaattggcat tataacagac tggaatttaa tatcatcttc cggatatatt agtaatggac
    29641 tcattacacc tgtaacacaa ataacaactg aaaacgaatt aatctctttt aataaatcca
    29701 gtaaaaattg cacattaatc cctattgtta ttggttcttt acaatctgaa tcaattgcca
    29761 gttcctcctg tccttcaccc tgatcaggat agcttgcttc tataagcaag gtattctgtg
    29821 aaaattttaa caatattctc catgaaggtt cattggtaaa attttttaat ctgttcaacg
    29881 cttcctttaa ttttttaact tcaataactg cctttaataa ataatcttta ggtataacct
    29941 gtttgtaatc aggaaactgc ccatcaataa ggcgtgaaac tatctctgtt ctccctattt
    30001 ttataaaaca tctgctatca tcaaaagcta taaggcaagt acctttatct tctaaaagcc
    30061 gcaatagctc attaactgtt ttaaggggta aaataaaacc attttttatg gtagaacttc
    30121 cggtaatatc tttctttatc attgataatc gccttgaatc tgtggcaaca actgtcatag
    30181 atttttcatc ttctgataca atatacacac cattaaatat tggttttacg gtatcatgtg
    30241 cagcagcatg aacagttttg cttatcattt cactgaaagt cttttgctct atttctatcg
    30301 catttttaaa attaaaatca ggtattgcag ggtattcttc agcagaagat cctaccagca
    30361 catactgtcc ttttactaga cccgattttg atgaaataga tacattggta ttattaacat
    30421 caagtattac tacaccatct ggaaattctt ttaataggct cactaaacgc ttcccgctaa
    30481 tagtgaagga acaattgcca tctgaaacaa tatccagctg tgttttaatt cctatctcgt
    30541 tgtcagtccc ggttatggtg agcgtatcac catctacatt aaaaagacac tgtgtaagta
    30601 ttgtattaat actttttgca ctcacaacag aatctgctat tattgttgcc ttgactaact
    30661 gtactttatc aacttcaatt ctcattgtgc acaccaccat tactattatt actaatttat
    30721 tataaactta ttaagataag tgttagtagt agttatataa tttttgtgaa taaataaaca
    30781 aaaggctgaa atcttcagcg tttttagaaa atgcattttg tgaataataa caaacgtttt
    30841 cataaagtat tataaaaatt taaaccctgt aaatgattta ctctacattt tgttgaaaga
    30901 tgcaacatta tcaataattc ttaacaattt tttaacaggt tattaacttc gtaattctga
    30961 tattaattca tcgataactt ctttaaaatc gatatcttcc tcgattgatt tttcaatatt
    31021 attgattgca ttaagtactg tagaatgatc cctatcacca aattcttttc caatctcaat
    31081 tgttgttaat tcggttaatc ttcgtgctaa atacatggca ataaaacgtg gctgaactac
    31141 tttactctgc ctattttttg aaataatatc atcaacactg ataccaaatt ttgaagcaac
    31201 ttttaccata atatcctgaa cagttatctt tttgttgctg tcaacgtcaa agaggtcctt
    31261 gcaatggtct tttgctaatt gtacagtaat gggttgattg tagatagagg aaaccatgct
    31321 taatctgacc aatgcagctt caagtgcacg aatacttgat tttattctgc gggctatgta
    31381 attgcatact tcgtctgaca attcaatttt aattttctct gctttatttc gtaatatagc
    31441 ttcccttgtt tccagattgg gtagctgtat atctacgatc ataccccatt caaatcgtgt
    31501 tcgaagtctt tcttcaagtg ttgaaagctc ttttggtggc ctatcactgg aaataactat
    31561 ctgctttttg ctgttatgta gtgtattgaa tgtatggaaa aactcttcct gtgtttgctc
    31621 ttttttttct ataaactgaa tatcatctat caacaaaata tcaacattac ggtacttaat
    31681 cttaaaactt gaaagcgtgt ttgctcttat agcctgaata aattcattta taaactgttc
    31741 acagggtaca tacagtacat taaggtatgg tcgttcctga atgattctgt gccctattgc
    31801 ctgcattaga tgtgtttttc ccagtccagt gccgccataa atgaacaatg gattgtattg
    31861 cgtggcgggt gcctttgata cggagatagc tgcggcatgt gccagttgat tattagggcc
    31921 aataacaaag ttttcaaatg tataatttgg gtttaatata gtgggaaatt gtaatgtctc
    31981 ttttttctca tcgttggtaa cgggcaaagg aacattcata actggctctt taaagccatt
    32041 gcctgtaaca aattcacata ctatctcttt acctgtatgt tctttgatga ttgaatgaat
    32101 caaagaggca taatgatcag aaatatgtcg ttttgcgaca tcgtcaggca ctttaacaac
    32161 aagaatattt tcattaaagg ccactggttc tgtattttta aaccacatat taaatgattg
    32221 cttattaata tatgtttcta cagaacccaa aaccttgttc cagattgtta ctgttgaatc
    32281 tgtcataata ataacccatt tagttatcaa caattgttaa taactttgtt gatatcgtaa
    32341 tatacgttta gcatatggta aaatggttag atgctacaga ctattagcga tgatgattat
    32401 tttgaattgt ctctataaaa gcattatgac ttttttttgc aaccaattaa tctataaaaa
    32461 ttattgcata cacaaaaaat tttatcatta tggataatgt gagaataaat actaaaaatc
    32521 tgtgcataat gtgtggataa ataccaatat atagttgaaa attatgaatt aatgtgtatt
    32581 atatgttaat aaaacaatgg tagaggaaaa caatgggtgt tatcattaaa gacataaaaa
    32641 aaaataacaa tgagattata cgagtggaaa tttcggaata tcagggaaag gaactcataa
    32701 atcttagaat atggtatcag gcaattgata atggtgaggt tgtctataaa cccacacaaa
    32761 aaggtgttgc actttctgtt gataaattca gtgagttaca ggaagcaata acaaagattg
    32821 cagaatatct gcatgataaa aagcacggta ctacacctca caatgtatag ttccattact
    32881 gtgtaatttg atcagggatt gtattaccaa ttgacgggct ttttttatct gcatttcatc
    32941 atgaactata tttggattaa gcacaccatc atagacatcc aggatacagt attgtgcccc
    33001 ttgtggaaac ttctgtatac aatcatatac aagctttttg ggtgtttgtt tcaatgcagt
    33061 ggcgatagta aaccacccac aatcaaacag tatataatac atcatctgag gatgaatata
    33121 tttctttaaa agttggttaa atggcattgc gtacagttta ttaatatgtg acatgttttt
    33181 taggtaaagc tggactattg tatactgtga caggattgtg tggttatatg tatatttgtt
    33241 ttgtaagtat acagctatac tgtttattcg ccgtgaagat agataccggg atagttgatt
    33301 gaaaagcttt tcattatcat taaaagctgt ggcgatagtt atatcgtcgc aatgtgataa
    33361 aatcgtatat agtaatccag attgttctat ttcagataac caggtttcaa atgttttttt
    33421 ctgaaatgtt gatacaatag caatatgatg aagaatgttg gtaatctgat caataaaatt
    33481 cccaaataca aaatatggtt ttgttcttaa ttttaaataa agaatttctt caaaggcata
    33541 tattccttca atcatgtctc gtgctgttat acgatgtata gttttcccaa catgaagaat
    33601 atccgatatc aggttacttg aaatattgta tttaattttt aatgcatgct gtggaaatgc
    33661 ccgtgttagc aaatacagat ggtaaggagt gagtatcttt tcattcaata aaaagtatat
    33721 caatttttgg aattcattat cttccattgt ttcaaggatt attggcaaag aggggaacag
    33781 gttgtaaggg tcgttgaaaa attgtaaaaa ttgtgcctca atatcattca atgaattgta
    33841 tggtgtataa ttgctaactt ttactctaaa tattgtgaat aattctagag gcatatatat
    33901 ggtaaaataa tcatcgacca ggtgtggagt tttacagcga atatatgcac taaataaaat
    33961 atgaacacta tcatatagtg tatggtcaag tggttcatta aaagcaaagg tagcctgtaa
    34021 gttgtttttt gataataaac ttaaacgcaa tgcaatactg gaaatgtttt gttgaatata
    34081 atcttttata actttgaaga tatcttcaga atcggttgta atgattactg gatactgttt
    34141 tgaggattgt ttcgttaata gtatttctgc atagtatttc tttacaattc tttttccagc
    34201 ctgccatggt gataatgcta tcccttcgca cacgccttct cttgaaacga aattatcaag
    34261 accatacaat aaactgtaaa aaagcaggtg tgatgcggta ttataaaaca ttgctataat
    34321 agttaaaatt atgaaaaatc aatgaacata aaggcaacat cgtcttttcg gtcatgaagt
    34381 ggagagaagt ggtcaagagt ctgcaaaaga tgctcacaaa aatcatcagg tttttcattg
    34441 tgttgtttca aaaattttgc caatctttgt tcaccaaatt cctctttatt atcatttttt
    34501 atttcagtta tcccatcagt atagagtatg cacctgtcac cttgttcaaa atggattgtt
    34561 ttttgttcat aaagtgattc atcctggata cctaagaata gaccattggt atcaagagca
    34621 ataattttgt cagagctttt tctatagtac agcggtcgtg tgtgcccagc attggtaaaa
    34681 gaaattgttt gttgttcagt gtcaataagc atataaaatg ctgtaatgta ttgcatatct
    34741 aaaagcagtc cgcgcatttc ctcattaatt tttgacatta attcatgagt atttaatatt
    34801 tcatatgcat aaatactaaa ggtcatcttt gccatgctca taaccagcgc agccggaata
    34861 ccatgcccgg taacgtcagc tattagtaca ccaacttttg aatcagagat tttgtagcaa
    34921 tcaaaaagat caccaccaat atctgcaata ggaagatatc gtccgctgat agatggatat
    34981 ggaacaaatg aaaaatcctt tggaatgata aattgctgga gtcgttttgc gtattccagt
    35041 tctttctgga tgagctgatt tttttcatat aattcatcat gggcgctttt taaacgcagc
    35101 aaggactgca ctcgtgctat cagttctttc tggtttaaag gttttaataa aaaatcatct
    35161 gctccaatag ttaatccctt aatcttggag tttaagtcat caaggccagt cataaggatg
    35221 acggggataa attcgttacc aatatattgt ttaaattgtt ccagaaaacc aaatccatcc
    35281 agattgggca tgacaatatc aactaatatt aagtcaaact gttttgactt taatgcgttg
    35341 agtccttcca tgccgtctgc tgcttcaaac acatgtatgt tatatgctga aagagtattt
    35401 tttacaattg ctctgtttaa tttgctatca tctataacca gtatgttttt tggtatacca
    35461 gatggatgaa aaggttgtaa aatcatagta tctacctctt cactttactt ttgttgatgc
    35521 tgaaaaaagc gcatattgct gttcaatata tggcccggaa gaatatgcaa tgttaatatc
    35581 ttttatgatt ctgtattctt catctttaag tatattagga gcataaagcg ggagattaat
    35641 ctgatcgcag aataatcgta aaccatttat aatttttatt ctgtctttaa agtaatggag
    35701 attgcgtatt atatgttcag ataatataac caatgaaaat tcaatagtag aaagtgactg
    35761 cagtaaatta ctcccaatgt aacagtttga aatagcaaat tcaacaggtg aggtgttctt
    35821 tgagcttaat ccctcatatt gcgggatgaa agagtgtaat tctatttcgt caatagctaa
    35881 aactatttta tccagtgaaa atctggtaca cactttttga agggtactta tctgtaaaga
    35941 aaggtctata gaagggaccc agtttatgat aaagggtaaa tggaatttgt gagccgatac
    36001 aatatttgcc aatgaagaaa taaactgtat gtaatgttcc tgagatgtaa ttgcgttttt
    36061 tatcattata tcaaaaaagt tttttgccga taccagctgc ttgctgaata catgacacat
    36121 tgatcgagca aattcagtat tatatactgg taataaaaat atataatcac tgatatcttt
    36181 ctttgaatga attgtctgcc aaaaagagga aataatttct gcttcatttg caagtgatag
    36241 caggggtgag aaattggatt taattgactt tatttcctga agtatttttc tggccagatg
    36301 tttatgaaat gggacaacac catgtgcata ctgaatttca acatgatgat gtattgattg
    36361 cagtgatata tccagtacaa gttctttgca gagtttggtg aattctgaaa cattagtttt
    36421 taatgatgat ggaaatggga agagtattgc tatctcataa tcataaagat ctgaaacagt
    36481 tacatatcgt tcgtatggaa aatgtatctc gcaaattttg gggatgagca aagaaatatt
    36541 ctcaatatca tattcaaaat ttttatatcc ccggtactgg taaaaatcaa caagtggtaa
    36601 cactttaatt attaacacac ctatagcatc tattgctgtt tcagatgaaa gtaagttttc
    36661 taagacaact ggaatgtagt gatatcctgt aacaggttcg tgaagaaatg ggtcaatgaa
    36721 gatgtttttt attattttat ttgttttaat atattcccag aatttatcat ttcgggacat
    36781 gattggactg aaagtataga cacggtttcg cccatcagtt tttgctgagt ataatgcctg
    36841 atctgcccgt gacaataatt ctgataaatt ggtgcccatt gaagggtatt ctgaaatgcc
    36901 ggcagaaaag ctgactgaaa atgtcttatc agggatattt gttttaaaat tgatgcttga
    36961 aaactggagt ctgatacggt ctataatttt ttttgcctct gttgcctgaa caccagggaa
    37021 aataacaaca aactcttccc caccaaatcg tgcaggtgta taattatctg attgcaatga
    37081 tttcagtata ttggctaact gaatcaggac tatatctccg atcggatgac cataggtatc
    37141 attaatattt ttaaagtgat caatatctat aattgctatt gaaacacagc tatgaatatt
    37201 gctttctatc tcaaatgaaa agcgacggaa aaattctttg cgggtaaaaa ggccggtgag
    37261 gtcgtcggtt actgataata gatacagttc atcatgctgt atcagttttg catgtaccag
    37321 ggaatacaac tcctcatttt taaatggtaa aggcaggtat ccatcggctc ctgaatcaat
    37381 agctgaaatt ctatcctgta tttcattatt atacccaaag actatgcagg gaacataacg
    37441 tgttatagaa tttttcttta taaggctgac aagctcaaat gcattgatgt cggtaatatc
    37501 ggtaccaatt aaaattatat gaggcaattt gtaagagatg aggcttaaca cttccatgcc
    37561 agtttttgta agtgtgatag tatataaaga atctgaaaaa accttagtaa tcgcactgac
    37621 agcgtcttca tcttttgaaa caataaaaat ctgataattt ttttctattg ttgttgtcat
    37681 tattcgttaa tatcccagtg tttcatttaa tatttttaat gcctcaactg ccgcttcatc
    37741 aaaatttttg gtatggtatt gtgattcctt atatgcatag ttaatactac ttgaagcatt
    37801 gactcgatgt aagcctttgt tttttgagtt gtttaatatt tttattactg cttccatgtc
    37861 acctccctga gtaccaatgc ctgggatgag aagtgggata tcttttccgg attcttcaaa
    37921 tactgtgata atattattga gttgctgagg aaatgttgca cctactacag caccaatacc
    37981 tggagcatac cactcaataa gtttttttgc aattaataaa taaagcggtt cattgttgac
    38041 aacaatatcc tgtatttctg aagagctttt attggaggtt ttaacaagaa tatagtatcc
    38101 tttgtcagga tagttgctgg taaatggctg tattgaatca aatcctaaat atggtgaaag
    38161 ggttacagca tctgctttaa agaattcaaa ggcttctttt gcataggcca tagcagtctt
    38221 cccaatgtca cctcgcttta catcaagaat cactgggagt ccttcattcc caaataacgt
    38281 aatgacatca cataaggcat gcaaaccttc aatgccgtac tgtgcataaa aagcgtaatt
    38341 tggctttaca catgcggggt aaatatggtg ttgcaaacat ttgttaagta taccctcgta
    38401 gaaggttaca atagtattgc gtatagaatc ctttctaatg ggaatatctt caagtaccgg
    38461 gtcaagcccc atacacacaa tacttttatg tttcctggaa ttgtatatca gcgattcaca
    38521 atagttcatt tacgaaagct cctgtaaatg ttttgtatag attgcataat tgtcatcatc
    38581 aaaaagcaca aaatacacgt ctattaaaaa attttctttt tccatgaact caatgatagt
    38641 tgataacgct atactacatg cttcttcttt tgggtatcca tacacaccgg tggaaatagc
    38701 tggaaatgca atggatgtac agccattgtc ctttgcaagt ctcatgctgt tataatagga
    38761 atttcttaat atagcagctt cattgtgact tcctccacgg tacactgggc caggtgtatg
    38821 aatgacatat ttagctttaa gcctgtagcc ttttgtaatt ttggcttctc cgggttgagc
    38881 accacccaat gtcctgcatt cttctaatag ctgtgggccg gcggcacggt gtattgcgcc
    38941 atcaacacca cctccgccaa gcaatgaagg atttgccgca ttaactatcg catcaacttc
    39001 catttttgtt atatcaccct tctggatatg gatatgtttt aaaatggtac gtttatctac
    39061 agccattggt acccccactg tgtaatatag taaaaaaatt tttttgcatg gtaaaaaaag
    39121 gtattattaa ttgataatag tactcacact ctatgtttaa ttcaagcata tttattgaaa
    39181 atgtttaaga gatatatact acaatgatac ggtattttga tattattgtt attggtgcag
    39241 gacatgcagg agctgaggca gcactggcat cggcacgccg tggatttgca acgttattgt
    39301 tgacaggcaa ccttgatact atctgtcaga tgagttgtaa ccctgccata ggtgggctgg
    39361 caaaaggaca tttagtgaaa gagattgatg ccctgggtgg ggaaatgggt cttgcaatag
    39421 atgagacagg tattcattat aagatgctca ataaaagcaa aggtcctgca gtgtgggcgc
    39481 cgcgggctca agcagataaa aagcaatatc agttacgtat gaaggaagtt cttgaaaaac
    39541 agaagaattt aactgttatt caggatatag ttgatgcaat tattgtagaa ggtgggcagg
    39601 tacagggtgt tgaaacaata cgcggacagc agcatgtagc taaagcggtg attgtatgta
    39661 caggtacatt tttgaaaggg cttatccata ttggtaatta taatgaaaag gcgggcaggt
    39721 tgggtgattt ctcggctgag aagctttcag attcactacg tagcctggga tttcctgtta
    39781 agcggttaaa gacaggaaca cctcccaggg taaatggtaa ttcaatagat tttagcaaat
    39841 gtgaagtaca gcatcctgat ccggtaccgg taccattttc atatagaaca cattccatta
    39901 atcgtcaaca ggtaccctgc tatattacct atacaactga aaaaacacat gcaattatta
    39961 ccaataatct gcatctttct ccattatatt caggcaagat acaggggata ggaccacggt
    40021 attgtccctc aattgaggat aaggttgtac ggttttcttc acgcaacagg catcaactct
    40081 ttttagaacc agaaggtttg cacaccaatg agtactacat taatggtttt tcaagctcat
    40141 tacctgaaga tgttcagtta gagatgataa gaaccatacc ggggctcgaa gaagtgcagg
    40201 ttatgcggcc agcgtatgct gttgaatacg attatgtgcc tcctgtagag ttgtaccaca
    40261 cactggaaac aaaaagagtt aaaggcttat accatgcagg gcaaataaat ggaacatcag
    40321 gatatgaaga agctgctgca cagggtatta ttgccgcaat aaatgcaggc aataagctgt
    40381 tagggatgcc accgcttgtg ttaaagcgtt cagaagcata tactggcgtc cttatagatg
    40441 atctggttac taaaggtgca gatgaaccat acaggatgtt tacttcacgg gctgaataca
    40501 ggttgctcct gaggcaggat aatgctgatg aacggttaat gcgctatgga tatgaaattg
    40561 gcctgataga taaagaatta tatgaaaaga cagtggaaaa gtataaacga attcaggagc
    40621 tcacagatag aattaataca gtttctattg ttattgatga taagttacag gaaatccttc
    40681 ataataaaca gattgcaata gaagaacgaa gccagataca agccggaaaa cttttgcgaa
    40741 gaccagaaat cagtcttgat gatgtgttgc catctattat acaaagcgat agtagccttg
    40801 taatgaatga acaggaagca aagattgttg agatgaacat aaagtatgaa ggctatataa
    40861 agaaggacct ggagcgtata aaaaagctgg aacagatgga aaacaaaaaa atacccgaaa
    40921 ccatcaatta tgatgaaatc attgggctta agaatgaagc acgggaaaag ctgaaaaaaa
    40981 taaggcctga aaccataggg caggcgcttc gtatttcagg cgttgatcct tcagatatat
    41041 caataattct ggtacatctt gaatcgttac acagaaaaca atccggttaa gttgctaatt
    41101 ttgtacatat atggtttata atatgattcc tgagattgaa tgttccacgt ggaacattat
    41161 gtcacattaa aatcccctga aagataacga ataaatataa tactgaaatt aaggctgtat
    41221 aaaaaaggtt attgagaaat attctacatt attattctta catattcaat tgcaaatcgt
    41281 gatgaggatt atcaatatct aattattaaa ggatatctat gccagatgtt ctgctttgcc
    41341 tttacttcag tttactcaca aacccattac atgatgatag agtgtcactg caatgtaaat
    41401 atcaaaatga ttctgccaac gctcgacaag gtgcttgcaa acccgaaaat aaaatttaat
    41461 caaatagttg aatagtataa tattgtgtga agtaatgatt aaaaacatga gaaaaggatt
    41521 ggtataaata tatactatct cataaaaagc tattaatggc tgcaaaaata ttgattaaat
    41581 aaaaaaacca ttattcctca aggtaattgc atttatatga aaataaacag tatataatta
    41641 atatgtttat gtattttaca taataataca atatacaggt tgattgatac atatagaaaa
    41701 cagatatgaa atagatattg aaaaaaagaa tggaagtatg aaaataatta ttgtttttaa
    41761 ttgaaaagat cgttgcaaag aagtaaggaa taaattctat tataacaaat ccataattat
    41821 ttctaaatct agatacaaga gaatagtaag tatgacaatt tcaaatacag taatcatacc
    41881 tgatgaaata aacagaaagc tgaaggaata tgcacgactt atatatgaac ataataaaac
    41941 gactaatatt actggattaa aggatacaga atccatttac aacgaactta ttctgggaag
    42001 cataacgcct ttgaagcatg tgaatgttcc acgtggaaca tcgtttatag atattggcac
    42061 tggtgcgggg atacctggaa ttccactttt gctgtatttt ggtggcaata taaaaggcgt
    42121 tctggtagat tcaaataata aaaaaattaa attcctgctg aatgcaataa atacattggg
    42181 tataaatgac atcgttacgg taattcaggg cagggtagag gagatagcaa aagaaaaggc
    42241 ctacagggat agctttgatt ttgctgtggc acgtgctttt gctcacccgc ttatggcact
    42301 tgagtatgga ttgccttttg taaagccggg aggatggctg tatatatatt attcctttaa
    42361 tctgtatgaa aaaggttttg aaaacaataa aacggtatgc agtaatgatg aagtaatagc
    42421 agagtatgga tgtgataaat tgcatgaaga aaaaaatgcc aataaacatg atataacagt
    42481 attaacggac tatttaacga agcattctct taatcttggc ggtcataggg taaccctaga
    42541 agaagccaaa aagcttaaac tatatgaagg tttgatattt ttgaaagatg ggtatacacc
    42601 agcaaactat ccacgaaaac atgctgttgt aaaaagggaa gttgagagtt taaggaatat
    42661 aagatcgtga gctatatgaa aaagaaattt cttctttgta tgttgttagt gtattttatt
    42721 cctctcacag gaattaatgc tcaaatacct caaggacaac tgaacagtat gatagtgaaa
    42781 gatgaaacgc tgattatacc tggcattggg gcatcatcta cagtgattga tatgtctgaa
    42841 caggaagttc tattaataaa agggaatcct tttaaaaaga cacaacatgt aacacatgat
    42901 tttttaaagg atgtgttaaa aatacaatct gaagcttcat tgccttttca ttatttatat
    42961 acataccgga atcctttaac aatcatagga ttctataatg gcagagtatc atttgttgtt
    43021 gtatgggact catatggtgt tttaattgat ggagtgcggg tatcaaaagg tataatggca
    43081 ataatattta attatggcaa tgaaggaatg caagttctca aagatgcaga tgggtcaatc
    43141 tatatgtatt cattaaaggg gatcgcgttt attgatgaaa agaatgatga tactgtagat
    43201 ggggtaataa tttttaagag ttgttcaaat taataattat tatgctattg aaatacctct
    43261 ggtaatgctt tcatggatat ggtgtgcaag catatcatta atcctatatt catgaaaaac
    43321 aatatcatca attgtatcca ttaatatttt tcgtttaact ctgtcagctt cttccctgag
    43381 ttctaaagat aattttttca taagcttttt gtttgtagaa ttaatgattt ttattggtag
    43441 tttctccaga ttaccacgta agactttaac agtaaaaaat tgagtttcat aaatatattg
    43501 gagtaggcga gaatttaaaa tagctacaag aacatcggga tccagctgtg gtgacagtgg
    43561 aataaaacca ttaacattgt taagtgtata tgatccatgc gggtcaacgg caaaggtaag
    43621 gtgtctgcca ataaatttat atagaatttt atttcttgta aggtaacatt gctgttcagc
    43681 aacctgttgt aatgtggata cattgtattt aaaatagtga ttgctgaaat taatacgaaa
    43741 tttttctaca tctttaccta tgattattgg gtcaggatgt cttgagtctt tgattgtgct
    43801 tatatggtag tcgttgttgc cagtgacaat gccaagaaaa aattttactc tgtcttgtaa
    43861 tgtaatatat gatagtgtat gaatgtgttc taatagtgca accgccttac gggtataatt
    43921 gatgggaaaa atagattcat gggtagtaca atacatttgt tggggtatca tgtgggcgat
    43981 agttgttaat gattttgcat gctttatgtg aacaatatgt ttctttcgaa tttcttcccg
    44041 ttcctccttt tgagcagtaa gcgatatggc aggtgcatac actcccttaa aacaatctcc
    44101 ccattgtact atttctttaa tcacagtatt tgaaagtact atttgccgtg agttctgatg
    44161 tgctttaata ttgaggtatg cttcagggat taaaaatgac aaaataccgc gttcatgcaa
    44221 aagtgaaagt gaccgttcaa taaataacgt aaaactgttt ataccactta ttgctgaaaa
    44281 ataatgctgt ttgaaatatt gcttttgttc atgtgtaaaa tgtgcgcccc agggagggtt
    44341 gccaataatg atatcaaata ctaaggaatt atgtttatta aaaagatcgt ctgttgaaag
    44401 catgtctctg tggaaaatgt tggtatgggt cccagaaagt ttttgtatat tccactgtgc
    44461 aatcatgcag gcaacaggat caatatcata gccaaataga ttattttgac agatggtgta
    44521 tgcagcatcc tcgtgagaaa tgccatgctg tttgtagagt tccagtaggt attgatatat
    44581 ataaatcaaa aactgaccag atccacaggc aggatccaga attttaactg taaaaggatt
    44641 gtcgtggtat tgcagcgcat gttcaaccat gtactgtaca atagcaggtg gcgtaaagta
    44701 ttgccctttc ttttttttgt catctgtatc tttgatattc tgataaatat accctgccag
    44761 atcgccatgc tgtattatgt cccattgagt cagtactctt gcaaggtcgt tgcatgctgt
    44821 gattccctca tttgataatg ggagtgttag ttttttctgg aaaagaatat gttccagtat
    44881 ttctgtaatg gcatcggttc tatttggtaa gagttcaata tcttttttta gttgcccgat
    44941 gcagaaatcg atataatgaa tgttatgggt aatatatgaa tcaatttttt tttgcatagt
    45001 aactatcggt tacaaaaatt gtagaataga ttgttcatat ttacatatcg atatattgta
    45061 taaaaacgat tagcgtttgt gaggaaacaa tgcacgaatt atccatcatg tccaatgtcc
    45121 ttgatattgt tgttcagcat gcatcacaaa atggtgcaag caaggtaact aaaatacatc
    45181 ttctggtggg ggaattgtct gattatattc ctgaatggat gcagacatat tttgattttg
    45241 taagtaagga taccattgcg gagaaagctg aactggttgt tgaaatggtg aaggcatcac
    45301 tgaaatgtga tgattgcggc aatgagtttc actttaataa aaatgactgg caatttagtt
    45361 gcccacaatg taattctcct aatgtaacca tcatttcagg tagggagctt actgtaaaaa
    45421 gtatagagat agagggatag tatgataagt attgcgatag ttgtggtaag tgacaggtcc
    45481 taccgaaaag agcgcgagga cctttctggc aaggcaatag aagagtgggc caggagcagg
    45541 aaatatagtg tggttgaatt ttgtattgtt cctgatgagc gcgaagtgat tgcacaaacg
    45601 ctacgggacc tgtgtgcaaa gaatattaat tgtattttaa caacaggagg aacagggttt
    45661 gctccccgcg atgtcactcc tgaggcaacc gctgatgtga ttgaacgcta tgctccagga
    45721 tttgcggaag ccatgcgcca tgcttccctt gcaataacca aacatgctat gctgtcacgg
    45781 gcaatagcgg gtatcagggg ctcttcaatt attattaatc taccaggaag cccaaaagct
    45841 gttgtggaaa atttagaggt tatacaggat gcactaccgc atgctgtcaa acttttacag
    45901 ggtcaggtta aagactgtgc tcacgagtaa aatttgattg tactgcccat tcatctatac
    45961 cgatactata tatagtacac atatcgttac tgtttatctg ttttaatact atcacgtgag
    46021 ttaattttag gaagaagttt tccagaagga taaatgtgtc aagcctggtt tgattagtag
    46081 taatgagttt ataaaataaa ttttttatta cattttgtta ttatgaaaaa atctatgaaa
    46141 caacaactaa catatgcagt tgtggttgta gtcacagtat tgctttatat accaggtgtg
    46201 ctggttgcag ggccaattgt gtcgattgaa cgtatcagtg tggatttatt tcctgagatt
    46261 accataaagg gtgttatacg caaccctgaa gggattcaga ttagtactat tggcagggaa
    46321 aattttgcca tcgtcgaaga taatacacat atcaatgatt ttactgtaaa ctcactggat
    46381 acagatacat atattgcact gtgtattgat gcaagcaaga gtatatcaaa gaaaaatttt
    46441 ttaaggctta aaaaaatagc cataaacatt ataaaaagaa gtaaaggcaa ttatacgttt
    46501 attgtatata agtttaatga tgaagttgta acactggctc cattttcaaa agatacgacc
    46561 aattacatag aaataattga atctttacag cagcatggca aacgaaccaa gctttataat
    46621 tgtttgtttg cagccacaga agaattagct cgccaaaagg ggggaaaatc cattattctg
    46681 ctaaccgatg gaaaagatga aggcagtttt atgttccctg atgatgttgt caataatgcc
    46741 cgtgaacata caattcccat ttttaccatt gtccctgaac ggtgttcaaa agccgctgca
    46801 gtaagcaggt taagtaaaat taccaaaggg aaactatttg ttgctactgc gggagttgaa
    46861 gaagcggtaa ttaatgtact gcaatcacgc atgctacata ccataccatt tgaaatcacg
    46921 tttaaaacaa ataaacatga tgatgccatt catcccatag aagtacaatt taaacacaaa
    46981 ggcatacagg ataatgatac gggatatgca aaatactctt ttcaaaagaa tacatataca
    47041 atacatactg atctgccatt tgtggtgctg gcagggattg ttgtgctttt ggtgatagcg
    47101 ctggttattg ttattctggt attaataaat aaatggaaac agatcgttga gcttgtaagc
    47161 atgcaacgaa taccacagcc cgttgaaaga tactattcta tgctctatga aaaagatgat
    47221 gctgtaaaaa aagatacaga tacactgctt cttgcaacat ctcctgaata cagttataca
    47281 aatgcatggc tggttgagaa aagcggacct gaaacaggga agaaatttcc tctcatatgg
    47341 gatgaggtaa cgataggcag agataaagaa aatgcgattg ttatcaacga tgatgctgtt
    47401 tccctgaaac atgcaaaaat taaaaaaatt gggaatacct actatctttt tgaccttgca
    47461 agcgataatg gcacattttt aaacaataaa aaattattgc gtcctaaacc tctctcagac
    47521 tgggatgaaa tacagatagg gcgaacaagt atccttttcc gcggaactaa gataaagcag
    47581 taaatactat gtcactcaag gtactgttgg tatcaccacc aatatttgat ttctattata
    47641 gctttcaccg ttcagaacca ttgggtcttt tatacattaa agaagcatta gcacactatg
    47701 attggcttac tgttacgatc tttgatgcgc gttacaaagg tacttctaaa gtaattacaa
    47761 caccagaagt tttcaattac cttcatcatc tgtatgtaaa agatacatca tggtttagcc
    47821 ttttccatgg atttaaacga tttggatatt cctataatac aatcgttaca tatataaaag
    47881 aaaatgaata tgatgttgta tgtattacct ctttattttc agcttatcat catgacgtgg
    47941 aggtgcttgt agaaagaatt aaaaatgaaa caggggcgat agttattgtt ggaggctggg
    48001 ctgtgtgggc tgatacaaac attctggtaa atggcagggc tgacttttat attcgtggag
    48061 atggcgagtt gacattgcct atactgctcc aggaaatata ttcttcaaat gggaacattt
    48121 cacatcttcc taaactcatt gatagtgaca actcatcggt caatgaattc tttatgcata
    48181 catttccgca tcgtgaaacc tggtatacat attatggtaa acggatagcc aatattattt
    48241 gtagtaaagg ttgtgtgtat cactgcgatt tttgttcaat tcactctcgg tatcgctact
    48301 atcagcgcac cattgattcc atacagcaag aacttgaata cctgtatgca cacggagtga
    48361 aaatagtcaa ttttgaggat gataactttt tatttaacag gaagtttgct aaacagttac
    48421 tatcgctcat gaaatactac cataataaag gaatgtattt cttatgtatg aatggtgtaa
    48481 cagctcccaa tctatatgcg gtgtttgatg atgccctgga tgctggattt ttagaattca
    48541 atctttcgct tgtttcatca tatgaagagg ttgttaagca acacaatcgt cccgctttac
    48601 aggagattat taaagacatt gcctggaaga gtgcaggcaa tgtcagggta atagtgtata
    48661 tcattgcagg gttgcctggt gctactgtta aaacatgtat cgatgatatt ctttttttag
    48721 cttcattgcc tgtgatgatt ggattttccc cactctatat gctgccaggg gtacgactat
    48781 ttgaaagtat gggagtgcct gatgacaggc gattgtgcag aggcagtgct ttgtattcat
    48841 ttggtgaagg ttttagcagg gaagatattg cagcactgtg gaagttatgc cgttttatta
    48901 acaggcttaa aattgctgat gcaattaatg atgaggtgcg cgtacattat gagttttaca
    48961 aaaaagcatg ccatgaccat gtatggtatt atcgagatga aaaaggattg tggcatgaag
    49021 gcttttcgtt ttctgcatcc cttcctgaaa gttttcatat atgtgatatc aatggtagaa
    49081 caaggctaat tggctgattt attttttata catgataaaa agataattaa atcctcgtaa
    49141 caaaaaattt ccctctaagg ctgctttatg gtagtttccc atctgaagtg tcctgttatg
    49201 gcgtactact gctatgatat caattggtgt gaaatagtgt gtcaacagtg aaaacaaatg
    49261 gaatccaata ggaacaaaac catctttttt attgtagtag tcgcatacgt ataagcccat
    49321 gtagcgctca tgctttaata ttctgtatat ctcttgtata acctgttcca tagcagtgaa
    49381 atactctttt tcagttgctt tcagtttgcc tatacaatcc ttttcatcag aataggtgat
    49441 gttatcacca tagggtgggt caatgaagac gaagtcagcc ttattgtttt caagaggcaa
    49501 tgcacgtgca tcagctttga tgatatctgt acggtaggga tggatatcat agcccaggca
    49561 ttttcttcct gtatcgttac aaacatcaat ggtggtacca ctgccacaca taggatcaat
    49621 aacaagatcc cccttttttg tatagcgcat gagaagattc cagatgatat atgacggtgt
    49681 ggctccaggg tagttctggt cgccctgttg cttattccca taatgctgtg aaggataatt
    49741 ccacaacgtt gtggtttgca tagttaatgg tggcttatgc atatactgct cccttgatag
    49801 taaactatac cagtagataa atatatcaat tattttaagc aattaatact aaaaaatagc
    49861 atagggatat taaactatat actatgtaat gcatttctat gttgtgaatt cctggtagag
    49921 aaaaattttc tcaatttcag atatatcaca gaatgagaag aaacaaaaca ctattaatgc
    49981 taaagaaagt ataaaaaata tcatttattt atttaaataa ttctggaaca atggtgtgaa
    50041 cattcccgca acctttgtaa gcagggatgt gcaatattta gttttttgga aagaagagtt
    50101 ataaaaataa taatacaaaa aataaggaat attttgggag aggcatatgg ataacaaaag
    50161 ttctgattgt attgctcaat gttatattct ttatggcaat tatgttaaaa gcattattgc
    50221 aaggttttat tcagataaag acgaaattga agatatattt catgatgttt ttttgaaatt
    50281 actcagcgct ggcttttgtg gtgatcccca ttcgcacaaa acaagaaact atatcagaaa
    50341 ggccaccagg catgtatgca tttcccgtat gaggaaagag atagcgcggc aaaagtattg
    50401 taacaatagt aatcctgata gcatgataga tatagaaact gtcactgata tgcaaaccat
    50461 ggatattggc gaaactgtaa ttgatggcat gattgtgagc actctctatg atgttattga
    50521 tgaacttgaa catgatgagc agaatttgat atttaataaa tatttttata acaaaaaata
    50581 tttccaattg gctgctgagc agggtgtttc atatcatgta ataaaaagaa agcttttggc
    50641 agttaataaa aagctaaagc ataaattgat gaataccatt gcatatcaat aaagcttaca
    50701 gatgttttat tatttagctt gaaatttatc gatatctatt atagaagtat tcagagttag
    50761 tttaaatata tattaaatca tagatatcgg aaggttttgc tttgaaatat ataattatct
    50821 tgtgttgttt tcttacaata ttttatgcgt tgtatgtagt tttggttaat gttctgaaac
    50881 ggttttcata tgcagtgaac aggtggtttt cgtttatagc attacttact gcagggatga
    50941 tttcatgcct gctagtgcct ttttttattc caaatattga gataactgag ctaacccgct
    51001 ggtactttgc cctaattata attattaatc aggccttctt ccattatacg cagatattcc
    51061 cacgctggga gaagaaaagc cctctgttca ttattataat ttcggcaatt ccaggagtga
    51121 ttactttttt gtatactgta gcaaccaata gtatcattct ttctgcttct tttgatggtc
    51181 tccatattca gtttgtgtat ggtcagttta tagtactgta tctgattgtt tttatttttt
    51241 atatcatggg aacgtttctc accttgtatt ataaagtacg acgcctggag aatacctctt
    51301 ttcggtacca gcttttttat gtgtttatgg gaaatcacat tggtgcactc ataatactca
    51361 tatcttttat cataatgccc tatgttttca aacgctttga atatcatagt ttaggaatat
    51421 ccttggcagc tttgatgtta ttatatatta ataactatgc tatctctgat gagcgatatc
    51481 ttaactttaa agaattttat cttaaaattg tttattatgg tgttgtgctg gtattcactg
    51541 taattccaac atattggatt cttgttaaca gttaccgtta ttttttggca ggaggccaga
    51601 tacctgcagt tgctgttgcc tttataaatt tcgtatattt aattttcttt actcgaatta
    51661 tacagccata tttagaaaaa tggattaaaa gggaatacgc aggattagaa aagcggttca
    51721 atgaatttat ccaggcattg tcacaggttg aggaatttga aggtgatccc acatactggg
    51781 atagattctt tacaaccaca atggattcac tggaatacct atttaatgtc acatctgctt
    51841 catttttaat gtataatgaa cctgaaaagg catatatttt aagtcatact ataggacaac
    51901 cgcctcaggt aaaaaggata tctgctgatg attcactggt tcaattatgc cagatagcaa
    51961 aaacaactat acatcaatca ttattttaca ccgatgaaat aataaagcca tttgatgatg
    52021 tactggcatt agtaaaaaac aattctattg aagttattct cccatccttt gaccagtcgg
    52081 gcaaactcat aggaatactt tttattggac ctcataaaaa gggtaggcca tatacattca
    52141 atgaaattga atttatggag ttgtacagag taaaatttaa cagtgcactt gttaattcac
    52201 ttcagattga agaggtaaag tcaaagcaga ttggcgaaca tgataattta gttgtgagtg
    52261 ctataaaaaa ttcaattatc ccccggcaga tgccctatat tgaccacatc aggataagct
    52321 cattttttat gaacaattca aatttagggg gggaattttt caatgcaaag gttttgcata
    52381 aaaatgcact agctttttta atatccgatt gctcctatag tggagtggat gctgcagtac
    52441 tggcattgga gctttatgct gcatttgaat ctttatctcc cacatatgac acgccggaaa
    52501 aaattatgca gttattgaac tgggtggtat gtacatccaa atttactgaa aggtatgcca
    52561 cttcattttg ttttacattt aattcggaat tactatcact gaaatatatt aacgcagcgt
    52621 ttaatccctt gattatatat gaaattcaaa atgatagttt tatagagtta gatactaaag
    52681 ggatccccat tgggatagaa aaagatttta tttatgaatc aaaggaatat gttatcaagg
    52741 gaccgtgtat tggttgtatt tactctaatg gcattaccgg tgctgtgaat cagacagggc
    52801 aaccgtattc cattggaaga attaaagatc tcataaggct ccacaacaac gagagcccgg
    52861 ctcttataat caggcgtatt tcttcagaca ttgatacata tacaagcaat acagcggtta
    52921 aggaagatat cagtattatt atcttccgaa tagaatgatt actcccaata aaatatatct
    52981 aattatagta gtgttatcat gattactatt aattttgaag aatcggccaa aaccattata
    53041 tcgttgttac agcaacagga aaatatagca attattataa aaggttctcc ggatccggat
    53101 gca
//
LOCUS       DAORYL010000011        52941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_527_length_52941_cov_13.243202, whole genome shotgun sequence.
ACCESSION   DAORYL010000011 DAORYL010000000
VERSION     DAORYL010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 52941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52941
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_527_length_52941_cov_13.243202"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(454..1191)
                     /gene="trpA"
                     /locus_tag="PLV81_03840"
     CDS             complement(454..1191)
                     /gene="trpA"
                     /locus_tag="PLV81_03840"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOM09268.1"
                     /translation="MTYNGFYLVGNYPDPQTFLQAAIEGLRYFDFIEIGLPFSDPVAD
                     GPVLASASHKALEAGVTTESVLQSCSALHEYCIKHNRSKNIYIMTYANKVFYNDIDTT
                     FEKFALHGIKGIILADVPYIESQRFAKRAKAHHLDYIHFITPESTPEQIKNICSQATG
                     FLYTVSLRGTTGSTLVITDEMQHILSTAKHFAKVPVVLGFGIQGKEDIIQALHYADGF
                     IMGTALVKKLEEGLDAYKDFLKEIFWR"
     gene            complement(1188..2363)
                     /gene="trpB"
                     /locus_tag="PLV81_03845"
     CDS             complement(1188..2363)
                     /gene="trpB"
                     /locus_tag="PLV81_03845"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOM09269.1"
                     /translation="MTYPDNNGYFGNYGGRFVPETLINALIELEDAYKHFLQDTSMQQ
                     ELNELLHHYAGRPTPLYFARRLSEQTGIVLYLKREDLLHTGAHKLNNTLGQALLTRFM
                     GKKRIIAETGAGQHGVATATVAALLGLECTVYMGAVDIERQMPNVKRMKLLGAEVVPV
                     TSGSKTLKDAINEALRDWVRNVRTTHYLLGSVSGPHPFPMIVRDFQKVIGNETKQQFM
                     TLTGKLPEFCVACVGGGSNAAGFFHAFINTSTRCIGVEAGGKGLYPGGHCASLTLGKP
                     GILHGALSYLLQNDDGQVMDVHSISAGLDYPSVGPEHSFWKDSNKVMYTTCSDKQALD
                     ACITLTRTEGIIPALESSHALGYVLENRNSFAGTSVIINLSGRGDKDLDTILKEVAL"
     gene            complement(2347..2976)
                     /locus_tag="PLV81_03850"
     CDS             complement(2347..2976)
                     /locus_tag="PLV81_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOM09270.1"
                     /translation="MFVKFCGFTRTGDVEAACRLGVSACGFIFYTGSPRFCNFMTAKE
                     CIAICKEYGVMSAGVFVDNSPNAIASIARALGLDALQIYSEDAYVELSPNFTIFLGIR
                     VGKNFSMHVIPVIDHKDFYLFDTFDSSQYGGTGKQFDWGSVASFAHINRTIISGGVNI
                     NNVQKLCTTLHPFGIDVASGIEDSPGIKNISKMKQLIQTIKECGHDISR"
     gene            complement(2966..3721)
                     /locus_tag="PLV81_03855"
     CDS             complement(2966..3721)
                     /locus_tag="PLV81_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HOM09271.1"
                     /translation="MFSLAMMKRKKLQQRADVINLLQKFPKRNRPIFPLVCDSSINII
                     AEVKKASPSAGTIKDVDPATQATLYQKAGAKAVSVLADSYYFNGSFDDVYHVADAVRL
                     PVLCKEFICYPEQIDIAYNAGADMILLIASLLTSDELHHLFTYTTSKGLLPLIEVHTI
                     SELDSVLTLQPDYIMVNIRNLNTLELEYDIAIATLKSIPDNIQTICASGINNPETLQR
                     IKEQTGTTTFLVGTALMKANDPQKLLQELSHVC"
     gene            complement(3725..4729)
                     /gene="trpD"
                     /locus_tag="PLV81_03860"
     CDS             complement(3725..4729)
                     /gene="trpD"
                     /locus_tag="PLV81_03860"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOM09272.1"
                     /translation="MNTEQLITQTIEGNTLSMEQSEYLFDSIFDGRISQIELAAVLAA
                     MKVRREKPEEISGAIKSMRKFAVSLPITESVFDTCGTGGDHSHSFNISSAVAIILNAM
                     GYPIAKHGNRSVTSKSGSADFYEALNIPVNLSGQDAVRYFNKHKFIFLFAPNYHPAMK
                     HAAPVRKQLGVRTIFNYLGPLTNPLQPKRQMIGVFHPSFLEVYAQAALPLGYERLILY
                     SSANGMDEVSPLHPTHVVEICNNKISTFTINPEKFLSNDEANAIPKDLSPQENATLFI
                     ETINNTTLTPLSKLLALNTALALFVLDDDSSIEDNYTVALDVIHSGSVAQTLTKLQEG
                     "
     gene            complement(4695..5288)
                     /locus_tag="PLV81_03865"
     CDS             complement(4695..5288)
                     /locus_tag="PLV81_03865"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOM09273.1"
                     /translation="MFCIIDNYDSFTYNLVEYMRILGEDVDVYRNDANFESINFHNYT
                     GIILSPGPSSPAHSGITVTVIQNIHDIPILGVCLGMQAIGYVYGGRIVHAKKIMHGKT
                     DIIQHYGGNLFHNIPQQFTAARYHSLVVSETDFPNCLSIEARSSDGEIMALKHKTKFI
                     WGVQFHPESYASEYGLQILKNFTGGSYEYRTAYNANN"
     gene            complement(5288..6781)
                     /locus_tag="PLV81_03870"
     CDS             complement(5288..6781)
                     /locus_tag="PLV81_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOM09274.1"
                     /translation="MYPTLQQCKSLCSTYNRIPIYKELNLSHPGLLLILKSFINNGDC
                     IFFESAKQNKKQSRFSFLGFNPAQTLLFSPPHIVISDSSQTKKILCSDPFDFIDTYIA
                     RYRSPSFKRFGSYNGGLSGYFAYDLVNYTGHLRQFIHQDTLHPLMVLHHIDDFICYDN
                     KYNTYYIATCIYTHDGSIESAYNQAIQCLHTYEDTIINTLSSTSLPYLPAYSESIDLD
                     FTSSPDEFMEKVSQAKTLIEDGEALQVVLSMRALINEPVDPYRFYLKLRQVNPSPYMF
                     YMKHGDLTVTGSSPEIHVKVQDTNATLRPIAGTIAQGKTKTQNKKNKEILLNNEKERA
                     EHLMLVDLARNDLSIIAKPGSVQVTQFMQPEDYSHVIHLVSNVTATLDDRISLSDVLR
                     HTFPAGTVTGAPKVRAIEIIDQLEPHPRGIYAGCVGYIGFNNTMDTCITIRTAVFSPQ
                     GSFLQAGAGIVYDSIPENEFNEIVHKLKALSVSLPFAKSKTMVKEIV"
     gene            7718..>8009
                     /locus_tag="PLV81_03875"
     CDS             7718..>8009
                     /locus_tag="PLV81_03875"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOM09275.1"
                     /translation="MLTFEIEALQNIKERLLQKIPDHFVCMYAFGSRVRGDFTADSDF
                     DVLIVVKNKTPQIEDTIVGVIVEEELQHGISFTPLIKDSKSFELEQQYHTPFY"
     assembly_gap    8010..8109
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8193..8600
                     /locus_tag="PLV81_03880"
     CDS             8193..8600
                     /locus_tag="PLV81_03880"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOM09276.1"
                     /translation="MTLSSEDKKTLSQFRLQKAKEFLDDAQKNFDDGRYRTSVNRSYY
                     AALNAARALLILEGANPETHDGVITMLSLRFVKKGILPSEVIKSFKLLLSRRTDVDYG
                     DFDSIDKEEALVSLNDAKTFIEAIEQAMKKILL"
     gene            complement(8651..11026)
                     /gene="lon"
                     /locus_tag="PLV81_03885"
     CDS             complement(8651..11026)
                     /gene="lon"
                     /locus_tag="PLV81_03885"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOM09277.1"
                     /translation="MPDDSNHVVESKHLILPSEILPEIIPIIPISHRPIFPGMMVPLV
                     LTGDTMLNTARAIAESEHKIGGVVLIQNQREGMVTSEDLYKVGVTIKVLKIAPIDENT
                     IQVMVNALKRFTLAQVVTEQPVIRWKVDHYYEPELEPTDEMKAYSAAILSSVKELIKS
                     NSLFQEELKLFLNRFSLEDPGKLADFVASLTSAEPAEVQDILETFDVKARVEKVLLLL
                     KKELELSKLQKKISQQIEERIAKNQKEFFLREQLKEIKKELGLEKDEKTSEIEKFEER
                     VKKLKLSEEALKRFNEELEKLKVLEPHSAEYGVSRNYLDWLTSLPWGIYSEDNYDIHK
                     AKKILDRDHYGLQDIKDRILEFISAGKKKGSITGSIICFVGPPGVGKTSIGKSVAAAL
                     NRKFYRFSLGGMRDEAEIKGHRRTYIGAMPGKIIQSLKVVETSNPVIMLDEIDKIGAS
                     FQGDPASALLEVLDPEQNSQFLDHYLDVRYDLSNILFIATANQLDTIPLPLMDRMEIM
                     HLSGYILEEKLEIAKRYLIPKQLKEHGLYKSEVKIDTAALKNIIDGYAREAGVRSLEN
                     NIKKIMRKATRQIIEGTTSTVTVTTQNLEEFLGKPRFTDESLYKKPIPGVVMGLAWTS
                     LGGATLYIEATAIPSKTKGFTQTGQLGNVMKESTEIAYSYIRSKVKQFGIDPNFFENH
                     FIHLHVPAGATPKDGPSAGITMATALYSLAKNKPIKKSVAMTGELTITGRVLPIGGVK
                     EKTLAAKRAKVKTIIYPAENKKDFDELPEHIKKGIDARFVSYFEEVLDIVY"
     gene            11267..12259
                     /locus_tag="PLV81_03890"
     CDS             11267..12259
                     /locus_tag="PLV81_03890"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOM09278.1"
                     /translation="MKLKYYSIPFLIAFVACCIHACKGQQKTLSGVIIFAQGDVTIIQ
                     NDVNKPAGIGANVNPNDIIATGNQSIAVVQIADRAIAHIGSNSRVVVQSLLDTSVTLY
                     LEKGEVISKVERLQKGQEYSVKARSVVASVRGTQFLVKADEKIGKVAVHTGSVSVKPI
                     IEAEEVEVAEIKETIVEGGKEAIVTVEEEKVAKEVPVTVQEISVKDKMKIETAAKIEV
                     VPQEVLAKPESLEKVQETIKQNVEKIEKIETLSESEIKQQIKKERIERLMQQKTRTLE
                     EIKEACERIDVVKLYSGKTIQGAIIDRTNDYKILTTTGVVEVPKKNVRSVSVLR"
     gene            12344..13447
                     /locus_tag="PLV81_03895"
     CDS             12344..13447
                     /locus_tag="PLV81_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09279.1"
                     /translation="MIKKYNINSLVQTVIVCVMIGIVFISNSAFAESIFLKDGSIIEG
                     DIVKETDKVTTVNTREGKTVTIQRKDILRTLVNESYKTKMYIMKSNKEVLGVYIVEED
                     NESYTYRTELQSAEEYKINKDDVLFVSKIPPKDFIDEEVQKKAAELGIKKKYTREENL
                     KWRAPFMRFGYSNFGVYVDSDVNDAYSDSRNINAFIDIFPWRFRNESGNGFDAMIRAR
                     LVGNFDEINPTDPRTQTFLKLYQVTLDESYESKLNIAQICGGVRYAYSMYYGVLIQPY
                     VYGLLQLFVFHDEIKIESLNSDAFDERVSSSKLGYQFGAGVDFGITSYVGLFIEGMYS
                     YIGAKFPDGKTRNVDGYYIYYGVSWRTSYGLIE"
     gene            13561..13758
                     /locus_tag="PLV81_03900"
     CDS             13561..13758
                     /locus_tag="PLV81_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09280.1"
                     /translation="MKKLERKIVAKKSLKEVESEYIEATPEERISMVWEITCDTWAFM
                     SDTNVERRLQRNITAVIRRRR"
     gene            13703..14185
                     /locus_tag="PLV81_03905"
     CDS             13703..14185
                     /locus_tag="PLV81_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09281.1"
                     /translation="MLNEDYKEILQLLSEEGAKYIIVGAYALAAHGFPRATGDIDIWV
                     KPDIDNAKKVIKVLKRFGCPLYDLTIDDLITKGSIFQIGVAPRRIDIITAIDGVEFDE
                     AWSDYITITIEDIKIPILSLDKLIKNKESTGREKDAIDAKYLKEFKNKKRNSDNVNNF
                     "
     gene            14340..14504
                     /locus_tag="PLV81_03910"
     CDS             14340..14504
                     /locus_tag="PLV81_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOM09282.1"
                     /translation="MDEIQYAPQLLRHIKVEIDINRKNNGQFFITGSQKFSLMEGVSD
                     SLAGRVGILT"
     gene            14537..14947
                     /locus_tag="PLV81_03915"
     CDS             14537..14947
                     /locus_tag="PLV81_03915"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOM09283.1"
                     /translation="MFFLDTGLLLYLPGIKSEKELLNSPLLGNIFETLCLGQIIRYYT
                     NQGINPDIYFFQDAYGHEIDFVIPSGEKLTLIECKWLFPLDKRKEIVSKIKQIIPQEK
                     IKKIVILSSQNEEAALEDKLFLYGCHDPDRFLRD"
     gene            15023..15442
                     /locus_tag="PLV81_03920"
     CDS             15023..15442
                     /locus_tag="PLV81_03920"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOM09284.1"
                     /translation="MSTVELKIKGYHLDLFGRVSNQRFLDFLEDARWSYLDTIGLNYD
                     EFAKRGVFLAVVNINVNFRAPSFLGDVLIIETMVDRIGNRSITVKQKMYNKKTEQIIL
                     DAEVVYVIVDLATGKSIPIDNEMRQLWLELSNKKNTH"
     gene            15520..16068
                     /locus_tag="PLV81_03925"
     CDS             15520..16068
                     /locus_tag="PLV81_03925"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOM09285.1"
                     /translation="MRKYIRYDKLSAEIKDAIYKYWEAAKQNKPDIALDDAMEDWFLH
                     QFDAFMIAKYHTQGDNMRKHFRLDVEIPIRVVELLIESSKDEAEALELIGTIVNISKG
                     GLYFISDIPLELSSIIRVVIDFRAVDNELTDIEALAMVVRHDRREDNRYGIGVMFSSI
                     YDNGKRNLNIFILKNLSYYLYS"
     gene            complement(16069..16509)
                     /locus_tag="PLV81_03930"
     CDS             complement(16069..16509)
                     /locus_tag="PLV81_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09286.1"
                     /translation="MISYIRLIFFSLLLYLPMICYISSCASKKTVTNIPPQENNALLF
                     EYEKEGFIDNNTFRVIVIIPAEEHYDELSIQQKGQERAFVSLKNYIISRNKVFDSKMH
                     NHLMTTITVYGTLKKRDSTCSTRYCYYYDITKSGLKAEIDTLGK"
     gene            complement(16530..17018)
                     /locus_tag="PLV81_03935"
     CDS             complement(16530..17018)
                     /locus_tag="PLV81_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09287.1"
                     /translation="MLKCLFSNVQPPFMNVPKTVTIIAFLLLIILLSCTHNQSYIKSL
                     SNDITTQGFLNDNCYQCIITAKPDPGIEGLVASRESAHINLMQSLEKQCYDTLINVTA
                     QLQGISVTDSFQQCLLQQLKQCIDNKVIVVTYFNEDFSETAVVRIYEKGLQQKLATLQ
                     CK"
     gene            complement(17101..19485)
                     /locus_tag="PLV81_03940"
     CDS             complement(17101..19485)
                     /locus_tag="PLV81_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM09288.1"
                     /translation="MAYYYDRYRNLKIAGVVAVILLLLFGGYYFLSRPKDALKDIVDI
                     RLKQIELYEKILETNPNDVDTLIKLGNLYKETGNLDKAIDYYKKALALQPDNYNALNE
                     LGHAYALKGDYENAIKTLELAKKYFPKYPQAYNKLGNVYDTQEKLDKALKEYKSAIAV
                     APQYQESYWNIAQQRMKKGDYKGAEEILKKGIKANPSDPEGYYNLGNLYMHKKQYAKA
                     IPQYQKAITLSPGTGKYYRALGNAYLAQNKIPEAVGAYSTALSKDPGDALSAERLGDI
                     YASQGNWAKAVDYYKQALSYNSNDKNLKNKYAHALSKLQKPSIIGGPDTQQLAVATKE
                     TTEPSAQQVISRDKDSKDLSQPTPQTTPVNPVNDSALKWRELGDKYRAEKKYDEALKA
                     YAKATQLDKTDDHSHYWMGRIYYHNQMDDEAKKSFQNAIIANPKNADSHYHLGLIYYS
                     EGKYSPAVNEFTKAIEEKPVFAKAYYARGLAQYKMNNLQAAIKDLKQSIEQDPNLDRA
                     YFNLGDIYLKQKNYKVALDYFTKDRELRPNNPDTLFKIAETYHEMKSYSNAETFYNKT
                     LEADPEYFQALFNLGLIAATKQNYTQAISFYTKALSIKNDDPPTLYELGKAYEGLGDD
                     NKAIEYYSLAITKNPNYTKAYINLGNLYKKNKLHDKAIEQFKQATINDPKSFEAHYNL
                     ANAYLSANMNDEAIEEYKTAIDLSPMNDQAHFFLGIAYKEKGVFEQAVKEFERSLQIN
                     SSLSDAHEELGMIYYRKLKNNEKAIYHFEKLLSMKPNHPKADQIRDIITMLKKQ"
     gene            complement(19514..20227)
                     /locus_tag="PLV81_03945"
     CDS             complement(19514..20227)
                     /locus_tag="PLV81_03945"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM09289.1"
                     /translation="MKKIALSCLITLLSVTIAYTISFAAKKDDDTPFKKGVKYFYQQK
                     FEIAELLFQEELKNNPENALAYSYLGDIFLYKKRYDGALQLYIKALELNPKSAEDAFR
                     IGQVYYYKKQPEEALAYFKKAFSLNPQLKFAYYHIGLTYLMLLRDKEKTIESWEKFLA
                     IAPEDPQYEKIKRVIELLKDPKFVLPPPGSDVSIEEALHLGGIILDDVQREAQDKRAG
                     HETKKTKNKLEEIYRDDEL"
     gene            20825..21979
                     /locus_tag="PLV81_03950"
     CDS             20825..21979
                     /locus_tag="PLV81_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOM09290.1"
                     /translation="MPKEGFKTAKQKNDCIEAIIQELVNRNGFLIVGHTNPDEDCVAS
                     MIAFSLLAKKFDKQVKIFSSKKIQENFKYLVDIAMYNRIDVVTAIPRSFAVDTIVICD
                     TAKPSMIDANRTILQMLKKSTIRKIEIDHHIGADSDYAGDPGYRLVTEASSASELVGQ
                     IALKLRAKKEILKKYVILDPLSRNLVLAILTGIIGDSQMGRYLKSSREKRMYRIFSTL
                     FNDILSTSTIKESNITNMNQIFEQLKQASEKEKHCFQFIKKKSKVYKSVGYAMLTEDD
                     MRYLYENFDDDDIISTTRTLADMLAEDSGKVGLVSYYDIPEKSNLVQFRMRRSQNYKN
                     YDLREFLTTMGIENGGGHEGAIGFRLPKHAVDDYPKFVTSLVKKINALLP"
     gene            22113..23954
                     /locus_tag="PLV81_03955"
     CDS             22113..23954
                     /locus_tag="PLV81_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOM09291.1"
                     /translation="MKRILMVLFYMILSVSVAMFSLQCAGKAVKQAEPVSTDEVPRDT
                     ALKEDDSDTTYSKGLKLEKEESFATKESAKTKALAPKKMDGAKTASGLKAGFADDNKQ
                     FNYFVNFIKDYKDKVQHYPINISERIIFYCKDIAGKSIPNAKVNVFSGKKLLETGKTY
                     ADGSFMFYPSEYDAKYTSYMLTFEALNTNAQITVDRQGKREHTVTLNTQRPQYKNVPL
                     DILFVFDTTGSMGEEIARLKKTIEIINLNIASLSTRPDVRFGMVLYKDKGDEYVTKII
                     PLTGHLEKFQKELSLVTAGGGGDGPEDLQSALADAVKKIQWNTGGIRLAFIITDAEAH
                     LDYGQQYTYVDAAHDAKKEAIKFYSLGTGGLPLMGEFILRQISQYTQGRYIFLTYGER
                     GESEGGKEGSVSHHTGANFQTDKLESIIIRFAKEELAYLTDQPLEEDEAYFDAKKVED
                     ETNEQTLQKLFDMAIAQLIDYSAINIQKGEPTAVLPIKSSDAALSTNAEYFTEQLSMA
                     LGKNKVFRQVERKNMQQILHEIGLGQTGIVDEASAAKAGKMLGAKMLITGTLYPKNAN
                     YEIFLKLIRVESAEVLSATKAIVDKNLGVAEGVKKPQPKQPVKKKTK"
     gene            23991..25352
                     /locus_tag="PLV81_03960"
     CDS             23991..25352
                     /locus_tag="PLV81_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOM09292.1"
                     /translation="MTSFADDVNTLRVVCQANTNPPVVSNAQVTRVVNALEVYCEFDI
                     VGTQYYIKHTKPDGTSTTTPFATAVLNSNNKPHVRKTFDMTGQAYGVHTFTIYAKDMY
                     NTTTYEVYTLQKDWQKLNIANAPVIWPKVEPGIGFITISWPAWYKEAEFGKYVIQMER
                     PVKTIEVTEYTTTSYKLTGLTPGDTYSGTFKVYDKAGNIIYSMQNPPRFFGHVGSIAP
                     KISNVSITPNPAGTNQEIQFLATVSDPDTNLTHVNLVLQEYVETTDSKGNLITQMGKT
                     ITLIDTDVYNTKQYQVIHHFVTSEPGKHKLILKATGASPNEFTQVEYSVIVQQGLDPS
                     TPKISIEGPNASLKQILTDGYQLKINIVSVDAESENIKAVIDWGDGTVKSEMIERESL
                     SISTTGKEGSPDFRKILSGSVSAKYYYQKPGVYVITVYIQSELKGKKLQSQPVRLKVR
                     VTE"
     gene            complement(25414..26709)
                     /locus_tag="PLV81_03965"
     CDS             complement(25414..26709)
                     /locus_tag="PLV81_03965"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM09293.1"
                     /translation="MTIVLFTVNFTTMSENIQYIYRSLSTLLPKETERKLVLITGARQ
                     TGKTTLAKKVYQHLRYINFDSPEIRQTVKNISTEDWAKDIGTAILDEAQKEPLVFEKI
                     KYSYDEQSLTFSVILGSSQILLLKKIRESLAGRIWIYELWPLMQSELVNITSWNVPLF
                     IKILESDTLDALLQQQPTVIVGQQMSNCKKAENYILQWGGMPALIHIYQNNEKHQWLK
                     NYIYTYLERDLSDLARLQDLEPFEKFQKLSALRCSSLLNYSELSRDAGISVDTARRYL
                     EYLKLSYQVILLQPYMENITSSTVKTPKIYWIDNGIMRQLTGYTSDTVTGQLYENHVI
                     SEMHKCIKTLQKPASLYFYRTRSGLEVDVVIETAKGLIFCEIKNREEIVKSDFTAIKS
                     IAQKLGKKFLGGLIIYRGERLYQLSHPSMWAIPSWRLFT"
     gene            26765..27943
                     /gene="dinB"
                     /locus_tag="PLV81_03970"
     CDS             26765..27943
                     /gene="dinB"
                     /locus_tag="PLV81_03970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOM09294.1"
                     /translation="MKDRIILHVDMDAFFTSVEQVDNPSLKGKPVVVGADPKGGRGRG
                     VVSAASYEARRFGIHSGMPISQAYKLCPGAVFLPPRFDRYVELSHIIMKIFERYSPCI
                     EQISIDEAFLDCTGTLKLFGNVENIARNIKTDVFTQTGLTCSIGIASNKLVAKIASDL
                     QKPDGLTICPCGMEKDFLAPLSIAKLYGVGIKTQKYLESSGYKTIGDIQRVDENFLQS
                     LLGKWGVVLHQFALGIDRRPVEPYSQAKSVSEETTFDVDTDDEDSIIFTLVELCDRVI
                     RRVRAAEFSFKTVTLKIRLEGFETYTRSKTLQQHARDMATLQKEIMRLYISFGRGKRK
                     VRLLGVKVSNLLCNADVQMNLFENQQKVEKIEEVIDSIHNRGLNVLRASVIKPPKKKG
                     "
     gene            27947..28360
                     /locus_tag="PLV81_03975"
     CDS             27947..28360
                     /locus_tag="PLV81_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09295.1"
                     /translation="MPKLLCDFVNSMIEEWGEDNPFYGLKNTGQLVEQWTNLDGLEIF
                     YSYVRNSKWVTVTVLPTETGIHPVSNSVYKWKGYINEYIAETAVWWAFELLTEMEAKK
                     FMIQHKPEVKFTFIRLGHPYELVVKFDGYYWVVED"
     gene            complement(28914..30335)
                     /locus_tag="PLV81_03980"
     CDS             complement(28914..30335)
                     /locus_tag="PLV81_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOM09296.1"
                     /translation="MNYWNEYKAKLTTPDDAVKVVECGDVVDYGFFNGKPVVLDQALA
                     RRAPELKDVLIYTAVTLPPVPEVIKHPESFTYIDWQWSKLTRLLHTQVEAAYYCPILY
                     HRAPFYYRYLLNQDPHENIDPGYRSFYYNRPEKSKHVKWIAMLQVGPMDDQGFFNIGP
                     QNSETSAKVDAADYVLVEVNKNMPRCLGGSEEAIHISRVDFIVEAPENQMLFAAPDVP
                     PTETDKKIAEHVMKFLYDGCCIQLGIGGMPNMVGKLIAQSDLKNLGGHTEMFVDAYVD
                     MIESGRMNGAKKNIDKFKCVYTFAIGSQRMYDFMHNNQALASYPVDYTNDPRIIAQND
                     DMVSICNAIQVDLFSQVNAESLGSGQVSGNGGMWDFVLGSQWSNRGKSFICLSSTYTD
                     SKGELQSRIVPQLPLASTVTIPRQMVDYIVTEFGAVKLIACPTWMRAEKLISIAHPKF
                     QDDLVKQAEKMKIWTRTNKQLAV"
     gene            30735..36296
                     /locus_tag="PLV81_03985"
     CDS             30735..36296
                     /locus_tag="PLV81_03985"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOM09297.1"
                     /translation="MTPTIQKGFIAVMIVLVICFGITTAYVLNTNEIKLTIDYDNNTP
                     YTELSLQFSRDIFIVSDIDAMKNDIAQKLTVSFNPELEYILKPLDSRNCILEIYNPKP
                     AHEYQFTIIVKNIDTKGLYVTANNKEIELGKTYFFRTPVLRVYHCSKESKMINSPIVL
                     QFNFPVSLNVLMQKMSIRPDVQYTLEYDSNDTSHKTVIVKPINEKKAQKYSLTIDKTM
                     VPASGVIGMDTSYTFTYTTYFPFELTDIQSSYSRYYDDSSYYPDYPVEFTFNNEIALP
                     QGKSVSEFVTVTPDPGGLQVNMYGNTIVVSGNFTGKKTYTVTIAQTLQDIYGQTLQEP
                     VETEIEFEHAFSYFSCPTGYMLLENYLEKIIPLRMVNVSSFECRYLSLVNENDIKAYF
                     ESPKEYFNKHAKTKTYILNWTWDVYKTVKFDYSKLHQGDNGIFVYELKPVMEHPYENN
                     HDVYRGMMQLSDIGVTIKKTKNDILVHVRDLLNNKALAGASLFCTGAEVSLGQTDAKG
                     VLIVKNNGCSLFTIKHGKSIFYYSMQRDNGYSDEDERAKINLPEKYYGSDYYILKPQV
                     HLFTDRYLYRPGETVHVKGILRYRNNDQWSIKPPLSVIKNVILTVINSRDEEIASSAR
                     SVSATGGIEWDIALKEDYPTGYYRITVSSSNKDYDLHQSIDFQVEEFKPAKAEMKIIP
                     SKLTFLWGEILSVDLFGWYLFGAPVNNDVEYTVTLQPVTYRSSVFADYSFSTQLNYYD
                     EYDEYSPPSTVVASGKVKVNEKGIAKVHVPLKAIANVPNAEILITGRTYLSDDSPVFG
                     AKSGLTVAEPVHVGIKVPRYFNYTKEPVTIQLVACNNDDTVVEGTPIKLVIKKHEWKS
                     FQIAGVNGRLQWEYKKIETEVNLQNHTIGKKDVVATINDPGYYTAEAYDSQGKRKYAV
                     CDFYVLGKGEAGWRVNDDETVDVETDKKSYNVGETAMLLVKNPFTKAQAFVSIEREKF
                     YDYFEVPATSSVVMIPVKITKEHIPNIYVSVMLYSGRTGFNALKDGEDRAKPRCLIGY
                     ARIKVTPKEKKLSVQVKPDKKQYEPGGYTTVELLVKDYKGQPVDGEATISIADRGVLN
                     LVNYTLPDPYEYFYAPRQLAVTTFDVHQIILGQRYLKEKGELIGGDGGIASMGMIVPR
                     ADIRFSAYYKATVVVKQGKAKVQCKLPDNLTSFKSMVVVHTEDSKFGYGEATFEVKKP
                     LMLLPTFPRFVRVGDTFNAGALVFNYTGKQGNVTIGLELKNGLKLKDGSAVAVKELIL
                     NNGQSQEVTFPVTVTGQAGMAGFTVKAQAGTYTDGITDTIPIKAPNVFETVAQYGRIS
                     APQSREVQEITITRNIIPEVSSINIFTSPSAFVDLKGCLDYLVDYPYGCFEQKASKIL
                     PLILGQDIIVQKGLLKTKSKDELRKVVTDVLKEFPAYYKNGGFSYWKDGSLPSSYLTV
                     YAAFIMTMAKKRGYDVDNAIYNDTIKVIKQFVKKGNIFSHEGRNSSWYTYTTLAFAHY
                     VAALQGFISENELKLVYSTITKKYDDCIAAYAYLLKALALYPDSTLKKTLVKEIAEKF
                     FSRMRTESGYVYFTDTSQWGYFYYDTVLSTALILQALLEANIPFNNDYKIVQYLLKQR
                     KGYAWLNTHQNAVVWWALNSYLVRYEKDKPDYSLQVLVDTKELLNAIVNESNVHFAKQ
                     YIVTSEDSGVKKFSFIKNGKGACYFTMQYQYIPVYEAMTSKNMGFEVSKLFKDIKTKE
                     TVKTFKKGSEYIVEITVYTPKDRGITVLEDQLPSGFEPVNVTFATEEKVAVDKKSDYT
                     NWGTFNHYEQYSDRIIYYADFIQRGTHKVIFKVRATNSGTFKLPACKVEEMYNPEVFG
                     ILYFSNTVTVE"
     gene            36299..38578
                     /locus_tag="PLV81_03990"
     CDS             36299..38578
                     /locus_tag="PLV81_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOM09298.1"
                     /translation="MKRYNWKHIDCTSANVLKASLVVFVVAIVTYIIFPYKDGYLTAN
                     NKSPITIYDRHGLVLMEITDGKKGLSQSVKIQEVPSEFIDLLLFSEDRNFYDHWGISL
                     KGIARALYQNLQVMHIVSGGSTITQQLVKSKKEIMRNTVVSKLAEIIEAIRLECHFSK
                     KEILQAYINEVYMGNNIYGFEKAAQVYFDKHLYQCDLLEQAFLISLVKAPERYNPYKN
                     PETIVKRARMLLDKASRNVIKLTPLQQEAYRNKKIHLQYDENTICAPLFCLYALSKAR
                     ELFPGKDITKIYTTLDAALYKDLLAVVRNSLSTLKDKNALHAAIVMIDNKTMEIMAMI
                     GSVDFFDYGKGQVDATLVKKQVASTMKPFAYALALDKGIFHTSSILPDVYTQFYSKVG
                     NYIPKNFSQSYHGPVRLAKALGCSYNIPAVYVINTVGMVPFYNFIRSIGFDSINRSPS
                     FYGLGIVLGNAEVTLLELANAYTIFPREGIFEHANCIRKIVDREGNEYVVTPDKGIRV
                     LQPSTCYLINHILSEYKYKVEAFGLQSAIHFPFPFAAKTGTSKDFKDNFVAGYNKNIT
                     CAVWVGNLYNETLDNLPAVSGAGIVLKNILLHLWNSGFPFAKFTFPTKEVHEVRICTL
                     SGLPATPFCPQTEYELYAHTVPKGVCTWHSSGKTTVPAEYVRWAAEKHYSVLKDSAVR
                     IIFPKNGATFKIDKTVRKSLQAIPLQAAGTKKEIQWFVDGHLIGKGSEIVWQLQQGNH
                     IICARSDKVAEYVNIVVLE"
     gene            38659..40752
                     /locus_tag="PLV81_03995"
     CDS             38659..40752
                     /locus_tag="PLV81_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOM09299.1"
                     /translation="MMHKLFYPDSVAIVGLSSRQNNIPRLTLENMLRWGYRGRIFGVN
                     PKSEDEQVDGIRMYKSIEALPEVPDLVYALVPAKFVPGIVEECGQKGVKWMAIPSGGF
                     SEYSEEGEKLAIQTVATAKKYGIHFVGPNGLTVANVENGLCLPFVPLLRPEFGGMSII
                     SQSGGVAIMMWNIIMDENIGIAKFASIGNKLDLDEVDFLEYLGNDPATSIICMYLESV
                     PRGADLIKVAEKINKPIVVYKSNTTQAGKKAAMSHTAAMSNDEEILNAAFEKAGIIRI
                     YNYHDFFAVAKAFKLPPMRGKRIMVMSPAGGFAVMTADLCEQAGFEFADPGEEFYQSL
                     QKFSNAGVIHFSNPLDLGDIYDPAFTAHVVYSVMHNQNVDGAVYVSQRPQMPDRESVF
                     SRMFLADLSKETWGAIVSSAKPLGICLLGLSRVMAQIKRTVNFPIFNSPEEMVRALAY
                     QMKFNTRQLRQKQDEPCTVSFDGAMQWIKRNTGEIGEDAMELLTTIDVPVPPSGIATT
                     EKEAVAIATRIGYPVVCKLVSKEILHKSDVGGVIVNVTSDDAVKQAFATIHANVLSHK
                     HDATIEGVRISKMAANGVDMFVGSKYDDSFGQVVLYGFGGIYVEVFKDVACSLCPAPK
                     DEIKEKISKLKSYALLKGARGQEPKDIDAYVDIIWKLSHLLARAPQIKEMDLNPVRVF
                     DNGCQVLDIRIKIVQ"
     gene            40788..41930
                     /locus_tag="PLV81_04000"
     CDS             40788..41930
                     /locus_tag="PLV81_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOM09300.1"
                     /translation="MIYLDNAATSYPKPQSVIKAVADCLQDYCANPGRSGHKLAIEAA
                     RKVFEARETVAQFFNVTDSRNVIFTSNATMAINCACAAILEDSDHVVVSSMEHNAVMR
                     PLRYLEKEGKISLSFIKSGSNGKITGDVTQLLQPNTRLVVINHVSNVNGVVQDIAHIT
                     AKIKAYSPSVTVLIDASQSAGVLPIDIQKIGCDFLAFTGHKALYGPQGIGGLIINTDR
                     PLKPFIMGGTGSKSESQYQPEFLPDKYESGTLNLPGIVGLKAGIEYVWPQMEKIRTEE
                     MKHIKKVIECLSGIKGITVYWSNTFEHQIGVVSFSHESISCSEIGQRLDDEYSICVRI
                     GLHCAPLAHETIGSYPDGTVRISVGTFTEDRDIDTFCRAIEQITKV"
     gene            41934..42185
                     /locus_tag="PLV81_04005"
     CDS             41934..42185
                     /locus_tag="PLV81_04005"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HOM09301.1"
                     /translation="MNYYLLLLPSVHDVMKAEKILLNAGVNVNVIATPREISHNCGVV
                     IRFESSNLHKLLVLLEELPLEKKVFKKVDDNVYVEVTQE"
     gene            complement(42172..42318)
                     /locus_tag="PLV81_04010"
     CDS             complement(42172..42318)
                     /locus_tag="PLV81_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09302.1"
                     /translation="MIQNNFDMKRWKLEQIKYFKKYQSTFPKNSKEYKILDESIKKLE
                     STLE"
     gene            42769..>43558
                     /locus_tag="PLV81_04015"
     CDS             42769..>43558
                     /locus_tag="PLV81_04015"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOM09303.1"
                     /translation="MINRDSIDIKDLPVFPQVAIKILQIQEDNIDISFKELESIILLD
                     PALTAKILKVANSALYARQREITNLQQALTLLGFKMVKSLVLLVSASNVYSKNVKSQQ
                     QSTAATVTKTSTAMIWRHSVVTAFLSKYVATRIKNDEKKEDVFVAGLLHDIGRLIMMI
                     HFRDKYEQYIAYLNQMQYKDIREVEEKIFEINHQDVGKIVLEKWNFPNELIDAVAQHH
                     VIQVDSKYKTTVQIVGLANLFAKIIENENLSENDRELLNAFSKAL"
     assembly_gap    43559..43658
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(43819..45195)
                     /gene="pelG"
                     /locus_tag="PLV81_04020"
     CDS             complement(43819..45195)
                     /gene="pelG"
                     /locus_tag="PLV81_04020"
                     /inference="COORDINATES: protein motif:HMM:NF028243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide Pel transporter PelG"
                     /protein_id="HOM09304.1"
                     /translation="MAGIGFELYKILHKGTLSSIVQAFFLGMIIVAGPWILSVLTIYI
                     IQTYTFGAIANNPALFTVSIVYVYAFSLFLSGGFHYVFSRYIADQLYIENYETIPTAL
                     LTAIIIITILSILPALAFVCFNDFSFIQFQTLYILSLVSLFVVINILWIMLVYIALLK
                     AYYKIFFSYLAGTIASIYGVLYLGKLYGVAGALCGYTIGQAVIVLLLIAISQITYPLK
                     KFTLNKEFIASFKEHKRIFLMGCFFNMAIWSDKMLYWFLQGQHIPGTLYYYYITYDIP
                     VFLAYLTMIPGLVYFLIIAETDFHKKFFAFISQILEDPFRYIQLKKQKMLLSLKNGIK
                     GIIFFQSIWTVGILIKLPEILEFLGYLHTINIHIMRILLIAVFFHMLTLNLMIYLLYM
                     ELQFEAAMAACLYLILNIAATLFSILYFPLLPGTSYMLASVATTLYCGYHLYKKAPII
                     DFIIFSRT"
     gene            complement(45202..46629)
                     /gene="pelF"
                     /locus_tag="PLV81_04025"
     CDS             complement(45202..46629)
                     /gene="pelF"
                     /locus_tag="PLV81_04025"
                     /inference="COORDINATES: protein motif:HMM:NF038011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GT4 family glycosyltransferase PelF"
                     /protein_id="HOM09305.1"
                     /translation="MDFVAINKPDPSKKTICIIAEGSYPYITGGVSAWIQDIITGLTN
                     YNFIIFAISAEEDEPKYLFPPNVIGLVNRLLTAPVEKPKKSIQSSFFSNIESFHNDMM
                     QKKDFSRFSEIFKLINKHRYDPSSLQRHITSFNIVTQYNEIRNPLYPFSDYYWACRSS
                     HEYMMKIMTWDLPPADLYHTISTGYAGLCAASAKIKYNKPVILTEHGLYNKEREIDIK
                     RATWVKGYQRDMWINIFNDLSAITYEYSDCVISLFEVNRNIQIAQGAKREKTLVIPNG
                     IEIDRYLDLKTETRKEFSVGIVARVVPIKDVKNFIIMAKIVAESIPDVKFYIIGPTDE
                     DESYYEDCVKLVENFQIQDKVVFTGKADVRQYYKFLDVVVLSSIREAQPLVILEAYAA
                     GIPVVSTRVGNVPEMLDYNDILLADPKDSEKLAQGVIYLYNNPDYRNKLIAANRNKVI
                     QFYNKKDLIATYDNLYAKYIGMYTQ"
     gene            complement(46617..47456)
                     /locus_tag="PLV81_04030"
     CDS             complement(46617..47456)
                     /locus_tag="PLV81_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09306.1"
                     /translation="MSTILPHEDFIFDYTVSKSSKNIIKLYSFENPFDFFRHDVVENP
                     YIVKTQHKLRKAFSEGLLGIEHYLMAATSLRPLIDEAQDLDEITRLFSQQKLAMRPNP
                     HSVKILRTMIKDPNPEIALYAAEGLNTIENSFLTKIEKIKKKLEKATHNIFIYHYLLG
                     LLYTQFAALLESQKLIQKFYIKQAIGNLVKAYALRPKNKRIKIALADSYLLDENFEYA
                     LSLYAYCYSIQYKPVYCLLKMAQCHYNLKHYDRIKTIASIISQYDVSDIEFAEVLIYQ
                     WIL"
     gene            complement(47465..48061)
                     /locus_tag="PLV81_04035"
     CDS             complement(47465..48061)
                     /locus_tag="PLV81_04035"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOM09307.1"
                     /translation="MGALNIEKIPFIKYNLYTEQLLLMIADLAAPIIRNAIRYSSLVH
                     KEVDPVTGFNSISEFYEVLKEEFANAQHNKLNLSLLIVECTNSAEIVEKFSMKDALLV
                     LKEIAQLVQQLAKGKVLLFQYKETFQFAAILPNMDFDGAAMFSLSLIEQNSKKAYYIK
                     GQIVNPEIVIGYSSLRPNHQSEEDLILLAENLLLMQKI"
     gene            complement(48025..48906)
                     /locus_tag="PLV81_04040"
     CDS             complement(48025..48906)
                     /locus_tag="PLV81_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOM09308.1"
                     /translation="MDIIVKIFKKIFPLEIILLVTAVFIFNILKYPDDIGFLSIHYNP
                     YLFVIIFFTSFYGKKSGLITFLTVTIIIASYIIISDLYYSTDILYTTITTPVSYQHLS
                     SLLFLSLIAIIILGEIRDNLGRIIQNQKNIIKELDEQAAKLKRELEAVSMVNQEYQDR
                     ILGQQNSLISLYSTIIALNTLHLENIYPNILNAVVQFTGSQQCSLWQYDRDNNSIHLL
                     AYNGWTQDIIDSTPSNLDTENLIGWSVRNNTMFSIKMLQKYQNLKALDNGNSIITVPI
                     TIDNQVWGRSTLKKSLL"
     gene            complement(49068..50279)
                     /locus_tag="PLV81_04045"
     CDS             complement(49068..50279)
                     /locus_tag="PLV81_04045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOM09309.1"
                     /translation="MRDAYIVQAVRTPGCRRGKGAFAQTRPEDLLVTALQGLMERAKV
                     DKKVVEDVMVGCAFPEAEQGLNVGRIAALMAGFPIDTCGATVNRFCSSGLESIAISAA
                     RIQAGWADVAIGAGLESMSIVPMGGNMPRPHPYWAKQKPDVYISMGITAENVANRYKI
                     SRQDQDEFAYHSQMKAAEAQKNKMFDEIVPTKAVRYVPQPDGTLKKEEFIQDFDDGVR
                     ADTTIEGLAKLKPAFAAFGSVTAGNSSQTTDGAAAVLLMSEEACRKYGVKPLVKLVAY
                     VVAGCEPDEMGIGPAVAIPKLLKLTGKDMKDIGLFEINEAFASQAIYCARQLGIGDKK
                     EWAVDSKDRRINVNGGAIALGHPLGCTGSKLASQLVNMMVKKGVKYGIESMCIGGGMG
                     AAALFELVEKW"
     gene            complement(50315..52720)
                     /locus_tag="PLV81_04050"
     CDS             complement(50315..52720)
                     /locus_tag="PLV81_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HOM09310.1"
                     /translation="MVRRIEKAAVIGSGIMGGGIAALLADAGVETLLFDIVPFNLTDE
                     EKKDPKARYRIVQAGFDNMMKAKPALIMDKSNASRISLCNLEDDFDKLKDCDLIVEVV
                     VENLKIKQELFQKIDKIRKPTAVVTSNTSGLPLKEMSKNCSDAFKEHFMGTHFFNPVR
                     YMHLLELIPGEKTKKEVLDFIAEFGEKRLGKGIVWAKDTPNFIGNRIGVHEIMTVMQL
                     LESEGITIDEVDAIMGPAMGRPKTAVFKLSDMVGLDTIHHLAENSYELLKNDERRDVY
                     KVPAWFNKMIENKWLGDKTKQGFYKKEKTADGKKLSLVLDPAKLEYREAAKPEFESVS
                     AAKKLPTAAQKIKAIVWGNDKAAKFAWKLTALGAIYAANRIPEIADSIVEIDNAMTWG
                     YNLELGPFQAWDAIGVKESVERMEKEGMKVPENVKKMLASGATSFYKVENGKEFYYDL
                     VNGGYKEIKKSAAAISLFNLKGAGKEIKKNESVSLIDLGDDVFCIEFHTKMNAINGQI
                     VDFIADATDFVLKNGAGIVIGNQAGGMPGAFSAGGDLAYMGSLAKEKKWSEIDKFIAN
                     VHAGLMKAKYAPIPVVAAPYGMTLGGGCEVCLNADRIVAHSELYMGLVEIGAGLLPGG
                     LGMLNLWKKYLNSVPKAAKITDLAGLFLPCFMAVAQAQVTMSAFEAQKKGFLTAKDRV
                     VFNRDLLIGEAKKEVLRMIDEGYAPPKKEKFVVIGREAMGMVEAEMLNMRIGMYITPH
                     MEFIAKKIAFVMSGGDVPSGTEITEEQWMKQEREAFVELWQTENTQKMAAHILQTGKP
                     LFI"
BASE COUNT        16589 a         9275 c        10505 g        16372 t
ORIGIN      
        1 gcgtcatagg gttgcgtgcg tgcttcgata aactcagcaa ccatggttcg acaggagatc
       61 actgagcgtg ttgaagtgct caccatcctt cattagatag gatcagtaca ggtgccgttt
      121 tttttgccga gcaccgcagg acacggcggg aaaagggcga ggactgttcg aagcaccgat
      181 gcaatcggtg caagttccgc agcccccgct gtggacgagg agcgcaggga atccctcatg
      241 aaatgaggga caaaaaacga taggcgccaa gccggggtaa aggggatgac aaggggaaaa
      301 ggggctggcc ggtcatcgag cctgtcgaga tgaagccctt tttccccttg tgaaaagaat
      361 attatggagt tgttgccgtc tggacaaaag aaagtccgaa tggaaagaat agttacatag
      421 actaatatca ggaaaaaaaa tttataacag taactatcgc caaaaaattt cctttaaaaa
      481 atctttatac gcatcaagcc cttcctctaa cttttttacc agagctgttc ccatgataaa
      541 cccatcagca tagtgtagtg cttgtataat atcttctttg ccctgaatgc caaatcccag
      601 aacaacagga acctttgcaa agtgttttgc agtggatagt atatgttgca tctcatcggt
      661 tataacaagg gtactgccgg tggttcctct cagtgagaca gtatacagaa aacctgttgc
      721 ctgtgaacag atatttttta tttgttctgg tgttgattca ggtgtaatga aatggatgta
      781 atccaaatgg tgtgcctttg cacgtttggc aaatcgttgt gattctatat acggcacatc
      841 agccagtata atgcctttaa tcccatgtaa ggcaaatttt tcaaaagtag tatcaatgtc
      901 attataaaaa actttatttg catatgtcat aatatatata ttttttgacc tgttatgttt
      961 aatgcaatat tcatgtaatg cgctacagct ttgtaaaaca gactcagttg ttacaccagc
     1021 ttcaagtgct ttgtgtgatg ctgatgcaag cactggtcca tcggctacgg gatcgctgaa
     1081 tggtagccct atctcaataa agtcaaaata gcgcaatcct tctatggctg cctgtaaaaa
     1141 agtttgcggg tcgggataat tgccaactaa ataaaatcca ttatatgtca tagtgccact
     1201 tccttcaata tagtatctaa gtctttatcg ccacggcctg aaagattaat gataaccgat
     1261 gtaccggcaa aactatttct attttcaagt acataaccca gtgcatggct gctttcaagt
     1321 gcggggataa tgccttcagt gcgcgtcagt gtaatgcacg catccagtgc ctgtttgtcg
     1381 gaacaggtgg tgtacataac tttattgctg tctttccaga agctgtgctc aggaccaaca
     1441 gaaggatagt caagccctgc agaaatggaa tgaacatcca taacctgtcc atcatcattt
     1501 tgtaacagat agcttaatgc accatgcaat ataccaggct tccctaaagt aagtgatgca
     1561 cagtgccctc cgggatatag ccctttgcct ccagcttcaa caccaataca tcgtgtagaa
     1621 gtattaataa aggcgtgaaa aaaaccagcg gcattgctgc caccgccaac acaggcaaca
     1681 caaaactcag gtaactttcc ggtgagtgtc ataaattgct gcttagtttc attgccaatg
     1741 actttttgaa aatcacgtac aatcattgga aaaggatgag ggccacttac cgaacccagc
     1801 aggtagtgtg ttgtgcgtac gttgcgcacc cagtcgcgta gcgcctcgtt tattgcgtcc
     1861 ttcaaggttt tgcttcccga tgtcaccgga acaacttcag cacctaaaag cttcattctt
     1921 ttaacattgg gcatttgcct ttcaatatca actgcaccca tgtaaacagt gcactccaga
     1981 cccagtagcg cagcaacggt tgcagtggcc acgccatgct gtcctgcacc ggtttcagca
     2041 atgatgcgtt tttttcccat aaagcgcgta agcaaagcct gccctaatgt gttattgagc
     2101 ttatgagcac cggtatgcag taaatcctct cgtttaagat acagcactat tccagtttgt
     2161 tcagaaagtc gcctggcaaa atatagtggt gttggtctgc ccgcataatg gtgcaataat
     2221 tcgtttaatt cctgttgcat ggatgtatcc tgcaaaaaat gcttgtacgc atcttccaat
     2281 tctatgaggg cattgatgag tgtttcagga acaaaccttc caccgtagtt tccaaagtat
     2341 ccattgttat cgggatatgt catgaccgca ctccttaatt gtttgtatta attgtttcat
     2401 ttttgaaata ttttttatgc cgggactatc ttctatgcca cttgctacat caatgccaaa
     2461 aggatgtaaa gtggtacaca gcttctggac attatttata tttactcccc cggaaatgat
     2521 tgttctattg atgtgagcaa atgatgcaac gcttccccaa tcaaattgtt ttcctgtgcc
     2581 tccatactgt gatgaatcaa acgtatcaaa aagataaaaa tctttatggt caataaccgg
     2641 aattacgtgc atggaaaaat ttttaccaac tcgtattcca agaaaaatag taaaattggg
     2701 cgatagttcc acgtaagcgt cttcagaata aatctgcagt gcgtccagcc ctaatgcacg
     2761 ggcaattgat gcaatagcat taggactatt gtctacaaaa acgccagcac tcatcacgcc
     2821 atattctttg cagattgcaa tacactcttt agctgtcatg aagttacaga agcgcggcga
     2881 gcctgtgtaa aaaataaacc cgcaggcgct aactcctaac ctgcatgcag cttcaacatc
     2941 acctgttctg gtaaagccgc aaaatttaac aaacatgtga taactcctgc agtagttttt
     3001 gtggatcgtt tgctttcatg agggcagttc caactaaaaa ggtagttgtt cctgtttgct
     3061 cttttattcg ctgtagtgtt tcaggattat tgataccact ggcacaaatc gtttggatat
     3121 tatctggaat acttttcagt gtggcaatgg ctatgtcata ttcaagttca agtgtgttca
     3181 aattccgtat attaaccatt atataatcag gttgtaaggt aagaacgcta tcaagttcag
     3241 atattgtgtg cacttcaata agtggcaata gtcctttact cgtagtatat gtaaaaagat
     3301 gatgtaattc gtcagaagta agcaaactag caataagcag tatcatatct gcaccagcgt
     3361 tgtatgcaat atcaatctgc tccggataac atataaactc tttgcacaac actggtaacc
     3421 taacagcatc agccacatgg tatacatcat caaagcttcc gttaaaatag taactatcgg
     3481 ctaaaacgct tacagctttt gcaccggcct tctgatacag tgttgcctgt gtggcagggt
     3541 caacgtcttt gatggtgcca gctgatggtg aagccttttt cacctctgca ataatattaa
     3601 tggagctatc gcacactaaa ggaaaaatgg ggcgattgcg ttttggaaat ttttgtagta
     3661 gattaataac atcagcacgc tgctgtaatt ttttacgttt catcattgca agtgaaaaca
     3721 tggtttagcc ttcctgtagt ttggtaagtg tttgtgcaac gctaccgcta tggatgacgt
     3781 caagcgctac cgtgtaatta tcttctatgg atgaatcatc atccagtaca aaaagcgcca
     3841 gtgctgtatt cagtgctaaa agtttgctaa gtggggtgag tgtagtatta tttatcgttt
     3901 caataaacag tgttgcattt tcctgcggtg ataaatcttt agggatagca ttggcttcat
     3961 cgttacttaa aaatttttct ggattaatgg taaatgtgct gatcttgtta ttacaaattt
     4021 caacaacatg ggttggatgt agtggtgata cctcatccat gccatttgca gaagaataaa
     4081 gtatcaggcg ctcatatcct aaaggcaatg cagcctgtgc ataaacttct aaaaatgaag
     4141 ggtgaaaaac gccaatcatc tgacgttttg gttgtaatgg attggtaagc ggccctaaat
     4201 aattgaatat agtgcgcaca cccaattgtt tccgtaccgg ggctgcatgt ttcatggcag
     4261 gatggtaatt gggtgcaaac aaaaatataa atttatgttt gttaaaatag cgcactgcat
     4321 cctgcccaga aaggtttacc ggtatattca gggcttcata aaaatcagcc gagccacttt
     4381 tgctggtaac cgaacggttg ccatgttttg caattgggta gcccatggcg ttaagaataa
     4441 tggcaacagc gcttgatata ttaaatgaat gtgagtgatc gccaccggtg ccgcaggtgt
     4501 caaagacgct ttcagtgatg ggaaggctta ctgcaaattt ccgcattgat ttaatggcac
     4561 cgctgatttc ctcgggtttt tccctgcgta ctttcattgc agcaagcact gccgctagtt
     4621 caatctgtga tatcctgcca tcaaagatag aatcaaacag atattcggat tgttccatgg
     4681 ataatgtgtt gccttcaatt gtttgcgtta taagctgttc tgtattcata agatcctcct
     4741 gtaaaatttt ttaaaatctg taatccgtac tctgatgcgt acgattcggg gtgaaactgt
     4801 acaccccata taaattttgt tttatgtttt aaagccatga tttcaccatc gcttgaacgg
     4861 gcttcaatgc taaggcagtt ggggaagtca gtttcactta ccaccagtga atggtaacgg
     4921 gcagcggtaa attgttgtgg aatgttgtga aataggtttc cgccataatg ttgaattata
     4981 tcagttttac cgtgcatgat tttttttgca tgaactattc taccaccata tacataccca
     5041 attgcctgca tgcccagaca cacacccaga atgggtatat catgaatgtt ttgaatgacg
     5101 gttactgtaa tgcctgaatg cgctggggat gaagggcctg gtgataaaat gataccggta
     5161 tagttgtgga aattaattga ctcaaaattc gcatcgttgc ggtaaacatc cacatcttct
     5221 cccagtatgc gcatgtactc aaccagatta taggtaaatg agtcatagtt atcaattata
     5281 cagaacatta cacaatctcc tttaccatag tttttgattt tgcaaacggc aggcttaccg
     5341 aaagcgcttt aagtttatgt actatttcat taaattcatt ctccggaata ctatcgtaca
     5401 caatgccagc acccgcctgt aaaaatgacc cttgaggtga aaagacagct gtcctgatgg
     5461 taatacaggt atccatggta ttgttaaatc caatgtagcc cacacacccg gcgtaaatgc
     5521 cgcggggatg cggttcaagc tggtcaataa tttcaatagc gcgcaccttg ggggctccgg
     5581 ttactgtgcc agcaggaaag gtatggcgca gtacatcact taaactaatc ctgtcatcga
     5641 gtgtagcagt aacgtttgaa accaggtgaa taacgtgcga ataatcttcc ggttgcataa
     5701 actgagttac ctgcacgctt cccggctttg caatgatact taagtcattg cgtgctaaat
     5761 caacaagcat caggtgttct gcgcgttctt tttcattgtt aagcaaaatc tctttgtttt
     5821 ttttattctg agtctttgtt ttcccctggg cgatagttcc tgcaattggc cttaatgtag
     5881 cattagtgtc ctgtacttta acgtgaatct caggcgagct tccggtaaca gtaagatcgc
     5941 cgtgtttcat gtaaaacatg tatggcgatg ggttgacctg cctcagttta agatagaaac
     6001 gatagggatc aacaggttca ttgatgagcg cacgcatgct cagcaccacc tgcaatgcct
     6061 cgccgtcttc aattaaggtt tttgcctgtg acactttttc catgaattcg tctggtgaac
     6121 tggtgaaatc taagtcaatt gattcagagt atgctggcag atatggcagc gaagttgagg
     6181 atagtgtatt gataatggtg tcttcatagg tatgaaggca ttgtattgcc tggttatagg
     6241 ctgattcaat gctgccatca tgtgtataga tacatgtagc aatgtagtat gtattgtatt
     6301 tattgtcata gcagatgaag tcatcaatat ggtgcagcac cattaatgga tgcagtgtgt
     6361 cctggtgtat gaattgtcgc agatgaccgg tgtagttaac caggtcatac gcaaaataac
     6421 ctgataggcc tccgttatat gaaccaaagc gtttaaaaga aggtgagcga tagcgggcaa
     6481 tatatgtgtc aataaaatcg aagggatcag aacagagaat tttttttgtt tgagaactat
     6541 cgcttataac aatatgggga gggctaaata atagtgtttg tgccggatta aaacctaaaa
     6601 acgaaaatct actttgcttt ttgttttgct tagcactttc aaagaagata cagtcgccat
     6661 tgttgataaa cgatttcagt atcagcaaaa ggcccggatg agaaagatta agctccttat
     6721 agatagggat gcgattgtat gtactacaca aacttttgca ctgttgtaat gttggataca
     6781 tggtattact ccttgctgta aaataaaaaa gccgtggatt ggtttaaccc acggccatag
     6841 tgccataaaa aagccgtggg tttgatgtcc acccacggct ttgccatttt catttataca
     6901 ataatagccc ttcaggtgga catcatgttt gtccaccacc accagctatt gttaactgca
     6961 aatgttacca gtgttatctt cattgctgtg ttacgtttaa tgcctttaaa attatatcgt
     7021 taaatgacat aaaaactaga aataaaaaat gtcaacaatt ttttattttt taatatttac
     7081 atcatctgtc tgatgtttat ctatgctctg ttccacaggg catgattgcg aaagtcaggt
     7141 acgaatacca agctttggaa atacataaat attcaaagca aaccacaatg ccataaggat
     7201 caaaaaccat attgcttctt tcatacgtgc accttttaca ttttatccac aggatgaacc
     7261 tgtatcaacc ataaaaccag caccatacgg tgtgtccaca aaatctatgg taatgttggt
     7321 tccagcatcc ataaaccgtg caatatcgct gtcatacatt acggggattt cgtttgcaat
     7381 tactactgta tcttttgtgt tttctgactc atccagagcc agtcccagac gtgggccgcc
     7441 tcatccaata ccggcaaaaa caagtcttaa tccaggctgc ttaaattcaa catctttggc
     7501 agcttcaata attttttgtt tagctgaatc tgtaatagtt atgttcatga taaaaccttt
     7561 acaataattt tatataataa taaaatacta acatttttgg gattttcaag tatttttttg
     7621 tttatatggc actaattatt gcttttttcc aatattgttg acaggcaatc atgtattttg
     7681 tatagtttta atacgtgaag taaaaggtaa agcatatatg ttaacatttg aaatagaagc
     7741 cttacaaaat attaaagaaa ggctattgca aaaaattcct gatcactttg tatgcatgta
     7801 tgcatttggt tcacgtgtac ggggtgattt tactgctgat tctgattttg atgtgcttat
     7861 agtagttaaa aacaaaacac cacaaattga agatacaatc gttggagtaa ttgttgaaga
     7921 agaattgcag catggtatat catttactcc actcattaaa gatagcaagt catttgaatt
     7981 agaacaacaa tatcacactc cattctatan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnna ttaaagatag caagtcattt gaattagaac aacaatatca cactccattc
     8161 tataataata ttgtaaatga aggtatacaa ttatgacatt gtcatcggaa gataaaaaga
     8221 cattaagcca attcagatta caaaaggcaa aagaatttct tgatgatgca caaaaaaatt
     8281 ttgatgatgg acggtataga acttcagtaa atagaagtta ctatgctgcg cttaatgctg
     8341 cacgtgcact tctgatactt gagggggcaa atcctgaaac acacgacggg gtaattacaa
     8401 tgctgagttt gcgttttgta aaaaaaggca tactcccttc agaagtgatt aaatctttta
     8461 aattgttgct atcacgcaga actgatgtcg attatggtga ttttgattct attgacaaag
     8521 aagaagccct tgtttcactg aatgatgcaa aaacctttat agaagcgatt gaacaggcaa
     8581 tgaaaaaaat actgttataa tagtaatatt gcttgcacta aagcctgtta acagtaacta
     8641 tcgctcacac ttaatacact atatccagca cttcctcaaa atagcttaca aagcgcgcat
     8701 caatgccttt cttgatatgt tcgggtaatt catcaaaatc ttttttattc tcagcagggt
     8761 aaatgatggt tttaaccttt gctcgtttag ctgcaagggt cttttctttt actcctccaa
     8821 taggaagcac tcttccggta atggtaagct cgccggtcat tgccacactt tttttaatgg
     8881 gcttattttt agcaagtgag tacagtgctg tagccatagt aataccggct gaaggaccat
     8941 ccttgggggt agcgcctgct ggtacatgca ggtgtataaa atgattttca aagaaattgg
     9001 gatcaatgcc aaactgctta actttggagc gaatatatga ataggcaatt tctgttgatt
     9061 ctttcataac attgccaagc tgtcctgttt gtgtaaaacc ctttgttttt gatggtattg
     9121 cggttgcttc aatatacagc gttgcaccac ctaagcttgt ccaggcaagc cccatgacca
     9181 cccccggaat tggcttttta tagagtgact catcggtaaa gcggggtttg cccaaaaact
     9241 cttctaaatt ttgtgttgtg acagtaactg ttgatgttgt cccttcaatg atttgtcggg
     9301 tggcttttcg cataatcttt tttatgttat tttccaaact tcgaacacct gcttcacgcg
     9361 catagccgtc tataatattt ttcagagctg ctgtgtctat cttaacctcg cttttataca
     9421 gtccatgctc tttaagctgc ttgggtatga ggtatcgctt tgctatctcc aatttttcct
     9481 caagtatata accggataga tgcatgattt ccatacggtc catcaaaggc aggggaatgg
     9541 tgtctaattg gttggcggtt gcaatgaaaa ggatatttga taaatcatag cgaacgtcta
     9601 aatagtggtc aaggaactgt gaattctgtt caggatcaag tacttccagt aatgctgacg
     9661 caggatcgcc ctgaaagcta gcaccaattt tatcaatttc atcaagcatg atgaccggat
     9721 ttgatgtttc taccacctta agactctgga taattttccc gggcattgcc ccaatgtatg
     9781 ttctacggtg acctttgatt tcagcttcat cacgcatacc accaagtgaa aagcgataaa
     9841 attttcggtt caatgctgca gccactgatt tgccaataga tgttttacca acaccgggag
     9901 gtccaacaaa acaaattatt gaaccggtaa tactgccctt ctttttacca gcactgataa
     9961 attctaaaat tctgtctttg atatcctgta gcccgtagtg atcacggtcc agaatttttt
    10021 ttgctttgtg aatatcgtaa ttatcttcgg aataaatgcc ccatggcaaa gatgttaacc
    10081 agtccaagta atttcggctt acgccatatt ctgctgaatg tggctcaaga acttttaatt
    10141 tttctaattc ttcgttaaac cgttttaatg cctcttcact taatttgagt ttttttacac
    10201 gttcttcaaa cttttcaatt tcagaagttt tttcatcttt ttcaagtcca agttcttttt
    10261 tgatttcttt aagctgttcc cgcaaaaaga attccttctg gtttttggca atgcgttctt
    10321 ctatttgctg tgaaattttt ttctgtaatt ttgacagttc cagttccttt tttagaagca
    10381 ggagtacctt ttcaacgcgc gctttaacat caaatgtttc aaggatgtct tgaacctcag
    10441 ctggttcggc tgaagtaagg gatgcaacaa aatcagctaa tttacctggg tcttccaggc
    10501 taaagcggtt taagaaaagt ttgagctctt cctgaaacag tgaattggat tttattaatt
    10561 cttttaccga tgaaaggatt gcagctgagt aggctttcat ctcatcagtt ggctcaagct
    10621 caggttcgta ataatggtca accttccagc ggataacagg ttgttcagtt actacctgtg
    10681 ccagagtgaa gcgctttaac gcattaacca ttacctgtat ggtgttttca tcgataggtg
    10741 ctatcttcaa taccttgatg gtaacgccaa ccttataaag gtcttcagat gttaccatgc
    10801 cttcacgctg attttgaatc agcaccacac caccaatttt atgttcagat tcagcaatgg
    10861 cacgcgcagt attcagcata gtatcaccgg taagaaccag tggtaccatc atgccaggga
    10921 aaataggcct gtgtgatata ggtatgatgg gaataatttc gggcaaaatt tcagatggca
    10981 gtatcaggtg ctttgactca acaacatgat ttgaatcatc cggcataggg tttcacctct
    11041 tgtaatctgt ttttgcttat aatgtagcat tgcaggttta aaaatcaaga taatttcttt
    11101 ttagctagtt ctctttggga gcaggcaaac atactgggtt ttagtcccat aggactaaaa
    11161 attttgaaaa aaaagacttt acatattaat tgaacaaaat aatataacaa aagcaaagag
    11221 tatattatat tatcattaaa actacatagt tccgtggagt ttaacaatga agctaaaata
    11281 ctactccatt ccttttttaa tagcttttgt agcatgctgt atacatgcct gtaaagggca
    11341 gcagaaaaca ctttccggtg ttataatttt tgcacagggt gatgtgacta ttattcagaa
    11401 tgatgtcaat aaaccagcag gtataggagc taacgttaat cccaatgata tcattgcaac
    11461 tggcaatcaa tctattgctg ttgttcagat agctgacaga gcaattgccc atattggcag
    11521 taacagtagg gttgttgtac aatcgctgct tgatacatca gtgacactgt atcttgaaaa
    11581 aggtgaggtt atatctaaag tagagcgcct tcagaaaggg caggagtatt cagttaaggc
    11641 acgttctgta gttgccagtg ttcgcggcac acaatttttg gtgaaagctg atgaaaagat
    11701 tggcaaggta gctgtccata cgggcagtgt cagtgtaaag ccaattatag aagctgaaga
    11761 ggtagaagta gcagaaatca aggaaacaat agtggaaggg ggcaaggagg cgatagtaac
    11821 tgttgaagaa gaaaaggtag ctaaagaagt gccagttact gttcaggaaa tttctgtaaa
    11881 agacaaaatg aagatagaaa ctgctgcaaa aatagaagtt gtaccacagg aagttttagc
    11941 aaaaccagaa tcgcttgaaa aggttcagga aaccataaaa cagaatgtgg aaaaaataga
    12001 aaagattgag acgttaagtg aaagtgaaat taaacagcag ataaagaaag aacgaataga
    12061 acgcttgatg cagcaaaaaa cgcgtacact ggaagagatt aaagaagcat gtgaacgtat
    12121 agatgttgta aaactgtatt cgggcaagac aatacagggg gcaataatag acagaacaaa
    12181 tgactataag atattaacta caacaggcgt tgttgaggtg ccaaagaaaa atgtacggtc
    12241 agttagtgta ttgcgataat gtttggaagt tttttaatta gtataaatgc attatatcaa
    12301 ttagtcatat gatgaaatac attatattat acaggagttt attatgataa agaaatataa
    12361 tattaactcc ttagtgcaga cagttattgt atgtgtcatg attggcattg tgtttatatc
    12421 aaattctgct tttgcagaat ccatattttt gaaggatgga agtatcattg aaggtgatat
    12481 agtcaaagag accgataaag tcacgacggt aaatacaagg gaagggaaga cagtcacgat
    12541 acagcggaag gatattttaa ggacgctggt aaatgaaagc tataagacaa agatgtatat
    12601 tatgaaaagt aacaaggagg tattgggggt atacatagta gaagaggata atgagagcta
    12661 tacgtacagg acagagttgc aatcagcaga ggaatataag ataaataaag atgatgtatt
    12721 atttgtttca aagataccgc ccaaggattt tatagatgag gaggtacaga agaaagcagc
    12781 ggaattaggg ataaagaaga agtatacacg ggaagagaac ctgaaatggc gcgcaccatt
    12841 tatgcgtttt gggtattcaa actttggagt ttatgtggat tctgatgtta atgatgcata
    12901 ttccgatagc cgtaatatca atgctttcat tgatatattc ccctggcggt ttagaaatga
    12961 aagcgggaat ggatttgatg ccatgataag agcacgtctt gtaggcaatt ttgatgaaat
    13021 taatcccact gatccaagaa cacaaacatt tttaaaatta tatcaagtta ctttagacga
    13081 aagctatgaa tccaaactca acattgctca gatttgtggt ggagtcagat atgcctacag
    13141 catgtattat ggtgttttga tacaacccta tgtgtatgga ttattgcaat tgtttgtatt
    13201 tcatgatgaa ataaaaattg aatctcttaa ttcagatgct tttgatgaac gggtgtcatc
    13261 gtcaaaatta ggataccagt ttggtgcagg agttgatttt ggaataacct catacgttgg
    13321 tttgtttatt gaaggaatgt atagctacat tggcgcaaaa tttcccgatg gtaaaacaag
    13381 aaatgttgat gggtattata tttactacgg cgtatcctgg cgcaccagtt atgggttaat
    13441 tgagtagatt attagtatag tatagtgatg aaaaatattt gactacagtg atctggcata
    13501 tagtgtagtg acaggccatg caagtatgca gtatattatt ataatttgta gtagcactct
    13561 atgaaaaagc ttgaacgaaa aattgttgca aaaaaatctt taaaagaagt tgaaagtgaa
    13621 tatattgaag caacaccgga agaacgtata agtatggtgt gggaaattac ttgtgacaca
    13681 tgggctttta tgagtgatac aaatgttgaa cgaagattac aaagaaatat tacagctgtt
    13741 atccgaagaa ggcgctaaat acataattgt tggtgcttat gcgctggcag cacatggatt
    13801 tccgcgagca acgggtgata ttgatatatg ggtaaaacct gatattgata atgcaaaaaa
    13861 agtaattaaa gtattaaagc gttttggttg ccctctttat gatcttacta tagatgatct
    13921 gataaccaaa ggctcaatat ttcagatagg ggttgctcca agaagaattg atattataac
    13981 ggcaattgat ggtgttgaat ttgacgaagc atggagtgat tatattacaa tcacaattga
    14041 agatattaag attcccattc tttcattaga taagttgata aaaaataaag aatcaactgg
    14101 tagagaaaaa gatgctattg atgcaaagta tttaaaggag tttaaaaata agaaaagaaa
    14161 tagtgacaac gtaaataatt tttaaagaga aaatgagtca ttgcaagaaa caggcgttgc
    14221 aatataatat atttttgtca tcctgagtgc agcgaaggat cttcttattt aagtgagatt
    14281 gcttcgctgg cgctcgcaat gatagtaggg tataaaaaag attcaaaaag cctttaatca
    14341 tagatgaaat tcaatatgca ccacagttat tgcgtcatat aaaagttgaa attgatatta
    14401 acagaaaaaa caacggacaa ttttttataa caggatcaca aaaattttca ctcatggaag
    14461 gtgtttccga tagcctggca ggacgagtgg gcattcttac atgacatacg ctttcactta
    14521 aggaaattca aacaaaatat tttttcttga taccggactt ttgttatatc ttccaggaat
    14581 aaaatcagaa aaagaattgc taaatagtcc tttgttggga aatatttttg aaacgttgtg
    14641 tttggggcaa ataatacgtt attataccaa tcagggtatt aatcccgata tatatttttt
    14701 tcaagatgca tatggtcatg aaattgattt tgttattcct tcaggagaaa aacttaccct
    14761 tattgagtgt aaatggttat tccctttaga taaacgaaaa gaaattgttt ctaagattaa
    14821 acagatcatt ccacaggaaa aaataaaaaa aattgtgatt ttgagttcac aaaatgaaga
    14881 agctgcatta gaagataagc ttttccttta tgggtgtcat gatcctgata gatttttacg
    14941 tgattgataa aaaactaatt gccaaaataa ttcctgtatg taaatataaa cggacaatgt
    15001 ggattccata agcaggtatg acatgtctac cgttgagcta aaaattaaag gatatcattt
    15061 ggaccttttt ggtcgcgtaa gcaatcagcg ttttctggac tttttagagg atgctcgctg
    15121 gagctattta gatactattg gcctgaacta tgatgaattt gcaaaacgtg gtgtattcct
    15181 tgcagtggtt aatataaatg ttaatttccg cgccccttcg tttctgggtg atgtgctgat
    15241 tattgaaaca atggtagata ggattggcaa tcgcagtata acagttaagc aaaagatgta
    15301 caataaaaaa accgaacaaa taattttgga tgccgaagtg gtatatgtca tagtggatct
    15361 ggcaacaggt aaatccatac caatagataa tgaaatgcgg caactgtggc tggagctatc
    15421 gaacaaaaaa aatacacatt aaaaaatttg attgctatgc ataccacttt actgtattat
    15481 attccacaag gttgagtatc cattatggaa gagggcataa tgagaaaata tatccgttat
    15541 gataagctgt ccgcagagat taaagatgca atatacaaat attgggaagc agcaaaacaa
    15601 aataaacctg atattgcatt agatgatgct atggaggact ggtttttgca tcagtttgat
    15661 gcatttatga ttgccaaata tcacactcag ggcgataaca tgcgcaagca ttttcgcctg
    15721 gatgttgaaa tacctatacg ggtggtggag cttcttattg aatcttcaaa agatgaagcc
    15781 gaagcgctgg agctaatagg cacaattgtg aatatcagca agggtgggct ttattttata
    15841 tcggacattc cgcttgagct ttcttcaatc atcagggtgg ttattgattt cagggcggtt
    15901 gataatgagc taactgatat tgaggcactg gcaatggtag tgcgccatga caggcgagaa
    15961 gataatcgat acggtattgg cgttatgttc agcagtattt atgataacgg caagcgcaac
    16021 ctcaatatat ttattttaaa aaatctttcg tattatcttt attcctgatt atttccccag
    16081 tgtgtcaatc tctgctttaa gcccgctttt tgtaatatca tagtaataac aataccgtgt
    16141 agaacatgtt gaatcccttt ttttcaaagt accatacacc gtaatggttg tcataaggtg
    16201 gttatgcatt tttgaatcaa aaactttatt tcgagaaata atataatttt ttaaagaaac
    16261 aaatgcacgt tcctggcctt tttgctgtat tgatagctca tcatagtgct cttcagcagg
    16321 aattattacg ataaccctga atgtgttgtt gtcaataaat ccttctttct catactcaaa
    16381 aagaagagca ttgttttctt ggggtgggat gttggtaact gtttttttgg aggcacatga
    16441 agatatataa caaatcatag gcagatacaa aagcagacta aaaaaaatta atcgtatata
    16501 agaaatcata ttaaccccaa ttatccttgt tatttgcact gtaaggttgc cagtttttgc
    16561 tgcaacccct tttcatatat ccgcacaact gcagtttcac tgaaatcttc attaaaatag
    16621 gtaactacaa tgactttatt atcaatacac tgttttaatt gttgtagcag gcattgctgg
    16681 aagctatcgg taacagatat accctgtaat tgtgcggtaa cattaataag ggtatcataa
    16741 cattgctttt caagtgattg cattaaatta atatgtgcac tttctctgga tgcaaccaat
    16801 ccttcaatgc caggatcggg ttttgcggta atgatacact ggtaacagtt gtcgttaaga
    16861 aaaccctggg tggtaatatc gttacttaac gatttaatgt atgattgatt atgtgtgcat
    16921 gatagaagga ttatcaaaag caagaaagca ataatggtaa cagttttagg aacattcata
    16981 aaaggtggtt ggacatttga aaataagcat ttaagcattg gtttaccatt aatatagtat
    17041 catcaatata atatacaaaa atataacaaa tcgttatatg tcaaataaaa aatggtattg
    17101 ttactgcttc ttcaacattg taataatatc gcgtatctgg tcagcttttg ggtgatttgg
    17161 cttcattgat aaaagctttt caaaatgata gatagctttt tcattgtttt taagcttccg
    17221 gtaatagatc atgccaagct cttcatgtgc atcagatagt gaactgttta tctgcagtga
    17281 acgttcaaat tcttttacag cctgttcaaa aacgcccttt tccttatagg caatacctaa
    17341 aaagaaatgt gcctgatcat tcattggtga aagatcaata gcagttttat attcttcaat
    17401 agcttcatca ttcatgttag cagataagta tgcatttgct aagttatagt gggcctcaaa
    17461 ggatttgggg tcattaatag tagcctgttt gaattgctct atggccttgt catgcagttt
    17521 gttcttttta tacaggttgc ctaagttgat gtaagcttta gtatagtttg ggtttttagt
    17581 gatagctaat gaataatatt caattgcctt attatcatca ccaagcccct catatgcctt
    17641 acctaattca tacagcgttg gtggatcatc attcttaatt gataatgctt tagtgtagaa
    17701 tgaaatagcc tgtgtgtaat tctgctttgt ggctgcaata agccctaagt tgaaaagtgc
    17761 ctggaagtat tcagggtcag cttcaagtgt tttgttgtag aatgtttcag cgtttgaata
    17821 cgatttcatt tcatgatagg tttcggctat cttgaacaat gtgtcaggat tattgggcct
    17881 taattccctg tctttggtaa aataatcaag tgccacctta taattcttct gtttaagata
    17941 gatatcacct aaattaaaat atgccctatc taagtttgga tcctgttcta tggattgttt
    18001 taagtcttta atagctgcct ggagattatt cattttatat tgagcaaggc ctcgtgcata
    18061 gtatgccttt gcaaatacgg gtttttcttc tatggctttt gtaaattcat taactgcagg
    18121 gctgtatttc ccttcagaat aatagataag ccctaaatga tagtgggaat cagcattttt
    18181 ggggttagca ataattgcat tctggaaaga tttttttgct tcatcatcca tctggttatg
    18241 ataatagatg cgtcccatcc agtaatgcga atggtcgtcg gttttatcaa gctgtgttgc
    18301 tttggcgtat gccttcagcg cttcatcgta ttttttttct gcacgatatt tatctccaag
    18361 ttcacgccat ttgagtgccg aatcattgac aggatttacc ggtgtcgttt gaggtgttgg
    18421 ttgtgataaa tcttttgaat ctttatctct gctaattacc tgttgtgctg agggttctgt
    18481 tgtttctttt gttgctactg caagttgttg tgtatcaggc ccaccaataa tagaaggttt
    18541 ttgtaatttt gaaagggcat gtgcatattt gttttttaga tttttgtcat tactgttata
    18601 gcttaaagcc tgtttgtaat aatcgactgc tttagcccag ttgccctgtg aagcatagat
    18661 gtcgcctaat ctctcggcag ataatgcatc gccaggatct tttgacagcg ctgtagaata
    18721 tgcaccaaca gcttcaggta ttttattctg ggcaaggtat gcattgccta atgcacggta
    18781 atatttacct gtgcccggtg aaagagttat tgctttctgg tattgaggga tagctttagc
    18841 gtactgcttt ttatgcatat acagattgcc taagttgtag tatccttcgg gatcagaagg
    18901 attggcttta atgccttttt tcaggatttc ttctgcacct ttataatcac cttttttcat
    18961 tcgttgttgt gctatgttcc agtagctttc ctgatactgg ggagcaacgg caatagcgct
    19021 tttatattct ttaagggctt tgtctaattt ttcctgtgta tcatatacat ttcccaattt
    19081 attataggct tggggatatt tagggaagta ctttttggca agttccagtg ttttaatggc
    19141 attttcataa tcgcctttga gggcataggc atgtcccaat tcgtttaatg cattgtaatt
    19201 atcaggttgc agtgccagtg cttttttata gtagtcaatt gctttgtcca gattacctgt
    19261 ttctttataa agatttccta actttataag ggtatcaaca tcattgggat ttgtttccag
    19321 aatcttttca tataattcaa tttgcttaag ccgtatatca acgatatcct ttagagcatc
    19381 cttgggtcga gaaagaaaat aatatccgcc aaatagcagt aatagtatta ctgcaacaac
    19441 acctgcaatt ttcagattgc ggtaacggtc ataatagtat gccataatgg tacctctggt
    19501 atgttaaatt atcttataat tcatcatcac ggtatatttc ttcaagcttg ttctttgtct
    19561 tctttgtttc gtgcccggcc cttttatcct gtgcttcacg ctgcacatcg tcaagtatta
    19621 ttccacctaa atgcaatgct tcttcaatgg atacatcgct tcctggtggt ggtaacacaa
    19681 attttgggtc ttttaataat tcaatgaccc tttttatttt ttcatattgt gggtcttctg
    19741 gagctatcgc caaaaatttt tcccatgact caatggtttt ttctttatcc ctgagcagca
    19801 ttaaatacgt caggccaata tggtagtaag caaatttaag ctgtggatta aggctgaatg
    19861 cttttttaaa atatgcaagt gcttcttccg gctgcttttt ataataatat acctgtccta
    19921 ttctgaatgc atcttcggcg ctttttggat tcaattccag agctttaata tacagctgca
    19981 atgcaccgtc atagcgtttt ttatataaaa aaatatcacc caggtatgaa tacgccaggg
    20041 cattttcagg attgtttttc aactcttcct gaaataagag ttcggctatc tcaaattttt
    20101 gctgatagaa atatttaaca ccctttttaa aaggggtatc atcatccttt tttgcagcaa
    20161 aggatatggt atatgcgata gttactgata aaagggttat cagacaggat aaagctattt
    20221 tcttcataga agtatttcca gtattattat cggtaatatg acgataacag aatacataaa
    20281 aacagtataa gcgcatcaac atgcaaaaac tgtaacaaaa aagaacagca tcattgcaca
    20341 aaaagctact ataaaccgtt tccactcaaa attcatgtag acctccattg aaaataatat
    20401 ttattctctg tgtcatttcg aattccgatt ttatcgggat gagaaatctt gaaagacttt
    20461 aatgtaattc tgaggataca aagtgcccga agaatctcat gattatagca ttatattctt
    20521 cattcccatt tcatcgggat gctgttgtaa aaaaaagatt tctcctcact gcgtttgtcg
    20581 aaatgacagt atgaaaacat tcgtcgaaat gacagtgtga aaacattcat caaaatgaca
    20641 gtgtaaaaac attcgtcgaa atgacaacgt ataaaacatg aataaccaca ttatttccat
    20701 gggcaactct ttaaaaataa tgaataaaat acatcctgtc aagcaacaca aaaataaaaa
    20761 aatatactgt tgacatttta aagatattgt tctacattta ctgccaatgt aaatggaaat
    20821 tcccatgcct aaagaaggat ttaaaacagc aaaacaaaaa aatgactgta ttgaagcaat
    20881 aattcaggaa ttggtgaacc gcaatggttt tcttattgta ggacatacta atcctgatga
    20941 agattgtgtt gcttctatga tagcattttc cctgcttgca aaaaaatttg ataagcaggt
    21001 aaaaattttt tcgtcaaaaa aaatacagga aaacttcaag taccttgttg atattgcaat
    21061 gtataaccgc atagacgttg tcacagcaat accccgctca tttgccgttg acacaattgt
    21121 tatttgtgat actgccaaac cttccatgat tgatgccaac agaactatac tgcagatgct
    21181 taaaaaaagc actattagaa aaatagaaat agaccatcat attggagccg atagtgacta
    21241 tgctggtgat cctggttaca ggctagtaac cgaggcatca agtgcaagtg agcttgttgg
    21301 gcagattgcg ctgaaactga gggcaaagaa agaaatctta aaaaaatatg ttattctgga
    21361 tccactgtcc cgaaacctgg tgcttgcaat tttaacaggc attattggtg attcacagat
    21421 gggacggtat ttgaaatcat cacgagaaaa gcggatgtac aggatattca gtacactgtt
    21481 taatgatata ttaagtacat ccacaataaa agaatcaaat atcaccaata tgaatcagat
    21541 ctttgaacag ttgaaacagg catctgaaaa agaaaaacat tgtttccagt ttataaagaa
    21601 aaaaagcaag gtgtataaat cagttggata tgcaatgctg acagaagatg acatgcgcta
    21661 tctctatgaa aattttgatg atgatgatat tatttcaact acacgaacgt tggctgacat
    21721 gcttgctgaa gattccggga aggtggggct ggtatcatac tatgatattc ctgaaaaatc
    21781 aaatctggtg caattccgta tgcgccgcag ccagaattat aaaaactatg atttacgtga
    21841 attccttact acaatgggca ttgaaaacgg tggagggcat gaaggagcaa ttggcttcag
    21901 gttgccaaaa catgctgtgg atgattatcc aaaatttgta acgtcattag taaaaaaaat
    21961 aaatgccctt ttgccatgaa gacaacaggt ttactttgtg ggtttctaaa tatttttcat
    22021 gacagaacaa tacagaataa aggtaaattt ctatagttca tcgtgattga tatgattttt
    22081 cagccacatc acatattttc cggaggaata gtatgaaaag aattcttatg gtattattct
    22141 atatgatttt atcagtttca gtggcaatgt tttcattgca gtgtgcaggt aaagcagtta
    22201 aacaggcaga gccggtaagc actgatgagg tcccacgtga tactgcatta aaagaagatg
    22261 atagtgatac aacatattca aaagggttaa agcttgaaaa agaagaatca tttgcaacaa
    22321 aagaatcagc aaaaacaaag gctcttgcac caaaaaaaat ggatggagca aaaacagctt
    22381 caggattaaa agctggtttt gctgacgata ataaacagtt caattatttt gtaaatttta
    22441 ttaaggatta caaagataaa gtccagcatt accccatcaa tatatctgaa aggattattt
    22501 tctactgtaa agatatagca ggcaaatcaa tacccaatgc taaggttaat gtttttagtg
    22561 gtaaaaaact tcttgaaaca ggtaaaacgt atgctgatgg atcgttcatg ttttaccctt
    22621 cagaatatga tgctaaatac acatcctaca tgcttacatt tgaagcgttg aacacaaacg
    22681 cacaaattac ggtagaccgt caggggaagc gtgaacatac ggtaacattg aatacacagc
    22741 gaccacaata taaaaatgta ccgcttgata tactctttgt gtttgacaca acaggcagta
    22801 tgggtgagga aattgcacgc ctcaaaaaaa caattgagat tattaacctt aatattgcat
    22861 ctttaagtac cagacctgat gttcgttttg gcatggtgct gtacaaggat aagggtgatg
    22921 aatatgttac taaaattatt ccgctgacag gtcaccttga aaaatttcaa aaagagcttt
    22981 cattggtcac tgcaggaggt ggtggcgatg gccctgagga tttgcaatca gcactggcgg
    23041 atgcagttaa gaaaatacaa tggaataccg gtggcattag acttgcattt attataactg
    23101 atgcagaagc tcatttagat tatggacaac aatataccta tgtggatgca gcacatgatg
    23161 caaaaaaaga agcaattaaa ttttattccc tgggcaccgg cggtctgcca ttgatgggtg
    23221 aatttatact gcggcagatt tcccagtata cacagggcag atacatattt ttaacgtatg
    23281 gtgaacgtgg tgaaagcgaa ggcggcaaag agggcagtgt gagccatcat accggagcaa
    23341 atttccagac tgataaatta gaatcaatta taatacgctt tgcaaaggaa gaattagcat
    23401 atcttactga ccagccactt gaagaagacg aagcgtactt tgatgctaaa aaagtagaag
    23461 atgaaacaaa tgagcaaacc ctgcaaaagc tttttgatat ggcgatagct caattaattg
    23521 actattcggc aataaatata cagaaaggcg agcctactgc agtattgccc ataaaatcaa
    23581 gtgatgctgc actatcaaca aatgctgaat attttactga acagctttca atggcactgg
    23641 gcaaaaacaa ggtatttagg caggtggagc gtaaaaacat gcagcagata ttacatgaaa
    23701 ttggtcttgg gcagaccggt atagtggatg aagccagtgc tgcaaaagca ggtaagatgt
    23761 taggggcaaa gatgctgatt accggcacat tgtatccaaa aaatgcaaat tatgaaatct
    23821 tcctgaaact tatcagggtt gaaagtgccg aagtactgtc agcaacaaaa gcaattgttg
    23881 ataaaaatct tggtgttgct gaaggagtaa aaaagccaca accaaaacag ccggtgaaga
    23941 agaaaacgaa gtaagcccat ggttacatcg ttgtgcaata atgagataac atgacatcct
    24001 ttgcagatga tgtgaataca ttacgtgtgg tatgccaggc aaatacaaat ccacctgttg
    24061 taagcaatgc tcaggttacg cgagtggtaa atgcacttga agtatattgt gaatttgata
    24121 tagtaggtac acagtattac attaaacata ctaaacctga tgggacttca acaacaacac
    24181 cttttgcaac tgctgtgctt aacagtaata ataaaccaca tgttaggaaa acgtttgata
    24241 tgacaggaca ggcatatggt gtgcatacat ttaccattta tgctaaggat atgtacaata
    24301 caacgacgta tgaagtctat actcttcaaa aagactggca aaaattgaat attgccaatg
    24361 ctccagttat ctggccaaag gttgaaccag gaataggatt tataaccata tcgtggccgg
    24421 cgtggtataa agaagcagaa tttggcaaat atgttataca gatggaaagg ccagtaaaga
    24481 ccattgaggt tactgagtat actaccacaa gctacaaatt aactggatta actcctggcg
    24541 atacgtatag cggaacattt aaggtatatg ataaagctgg aaatataatt tacagcatgc
    24601 aaaatccacc tcgatttttt gggcacgtgg gttcaattgc tccaaaaatt agcaatgtat
    24661 caattactcc aaatcctgct ggaacaaatc aggagataca gttcctggca acagtaagtg
    24721 atcctgatac taatctaact cacgttaatt tagtgttaca ggagtatgta gaaactactg
    24781 attcaaaagg caatctcatc acacagatgg ggaaaaccat tacactaata gataccgatg
    24841 tgtacaatac taagcaatac caggtgattc atcactttgt taccagtgaa cctggtaaac
    24901 ataaacttat attgaaagct actggagcat ctcccaatga atttactcag gtagaatata
    24961 gtgttattgt acagcaaggg cttgatccat caacgccaaa gatttcaatt gaaggaccaa
    25021 atgcttcgtt aaaacagata cttactgatg gctatcagtt aaagattaat atagtatcag
    25081 ttgatgctga atcagaaaat attaaggctg ttatagattg gggtgatggc accgttaaaa
    25141 gcgagatgat tgaacgagaa agtttaagta tttcaaccac tggcaaagaa ggttcacctg
    25201 atttccgcaa gatattatct ggctcagtat ctgctaaata ctattaccag aaaccaggtg
    25261 tatatgtaat aactgtgtat attcaatcag aattaaaagg caaaaagcta caatcacaac
    25321 ctgtacgatt aaaagtaaga gtaaccgaat aataagttgt ataaaaatac cgtaataagc
    25381 ccatctcatt cgacaagaga tgggctttta attttatgta aataaccgcc atgaaggaat
    25441 agcccacatt gaaggatgag aaagctgata taaacgctct ccacggtaga tgataaggcc
    25501 accaagaaac tttttcccaa gtttttgagc aatagatttg atggcagtga aatcactttt
    25561 gacaatttct tccctattct taatttcaca gaagattaat ccctttgcag tttcaataac
    25621 aacatctact tctaatcctg atctggttct gtaaaaataa agtgatgctg gcttttgcaa
    25681 tgttttaata catttgtgca tttcagaaat aacatggttt tcatatagtt gtcccgttac
    25741 tgtatcgctt gtatatcctg ttagctgtcg cataattcca ttgtcaatcc agtatatttt
    25801 aggtgtttta accgttgaac ttgtgatatt ttccatatat ggctggagaa gtataacctg
    25861 atatgatagt tttaaatatt ctaagtatct gcgggcggta tctacactta tgccagcgtc
    25921 tcttgataat tctgaataat ttaacaatga ggaacatcgc agtgctgata gtttttgaaa
    25981 cttttcgaat ggttcaagat cctgtaaccg agccagatca gagagatcgc gttctaaata
    26041 ggtatagata taatttttca accactgatg cttttcgttg ttttgatata tatggatgag
    26101 tgctggcatc ccaccccact gtagtatata gttttcagct tttttgcagt tgctcatttg
    26161 ctggccaacg ataactgttg gttgctgttg taatagagca tcaagagtat ctgattccag
    26221 aatctttata aaaagtggga cattccatga agtgatattt acaagttcag attgcatcaa
    26281 tggccataac tcatatatcc atattctacc tgcaagtgac tctctgattt tttttaataa
    26341 aagtatctga cttgaaccta atataactga aaatgtaaga gattgctcat cgtagctata
    26401 tttaattttt tcaaaaacaa ggggttcttt ttgtgcttca tcaagtatag cagtgcctat
    26461 gtccttagcc cagtcttctg tggaaatgtt tttgactgtt tgcctgattt ctggtgagtc
    26521 aaaatttatg taacgaagat gctgatatac cttttttgct aaagtagttt tgcctgtctg
    26581 acgtgcacca gtgattaaca caagctttct ttcagtttct ttgggtaata aggtgctaag
    26641 acttcgataa atatactgta tattttcgct catagtcgta aaatttacag taaataatac
    26701 gattgtcaag aattttttta atttctcctt gacagtagta ctatatatcc gtatagttat
    26761 ttttatgaaa gatcgaataa ttcttcatgt ggacatggat gccttcttta cttcggttga
    26821 gcaggtggat aatccttcgt taaaggggaa gcctgttgtg gttggggctg atcctaaagg
    26881 tggaaggggc cgcggtgtag tttcggctgc aagctatgag gcacgtaggt ttggcattca
    26941 ttcaggaatg cctatttcac aggcatataa actatgccca ggggctgtct ttttgccccc
    27001 acggtttgac cgatacgttg aactatcgca cataattatg aaaatttttg agcgctattc
    27061 cccctgtatt gaacagataa gcattgacga agcattttta gattgtacag ggacactgaa
    27121 actgtttggg aatgtggaaa acattgcacg taatattaaa actgatgttt ttacacaaac
    27181 agggcttacc tgcagcattg ggattgcgtc aaataagttg gttgccaaga ttgcgtcaga
    27241 tttgcaaaag cctgatgggc ttacaatatg tccctgtgga atggaaaaag attttctggc
    27301 accactgagc attgcaaaat tatatggtgt tggaataaaa acacagaaat atttagagtc
    27361 atcaggatac aaaaccattg gggatattca gcgtgttgat gaaaactttt tgcaaagcct
    27421 gcttggaaaa tggggtgtag tgttacatca gtttgcactg ggtattgata gacgtcctgt
    27481 tgagccatac tctcaggcaa agtcagtgag tgaagaaaca acctttgatg ttgatactga
    27541 tgacgaagat agtattattt ttacgctggt tgaactgtgt gatcgtgtta tacgaagggt
    27601 cagagccgct gaattttctt ttaagacagt gacgttaaaa atacgattgg aaggttttga
    27661 aacatataca cgcagtaaaa cattacagca acatgcccgt gatatggcaa cactgcaaaa
    27721 ggaaatcatg cggttataca tttcatttgg tcgagggaaa aggaaagtgc ggcttttagg
    27781 ggttaaggtt tcaaatttac tgtgtaatgc tgatgtgcag atgaaccttt ttgaaaacca
    27841 acaaaaggtt gaaaaaattg aggaagttat tgatagtatt cataacagag gacttaatgt
    27901 gttacgagct tcggtgataa aaccgccaaa aaagaagggg tagagtatgc caaagctttt
    27961 atgtgatttt gtcaacagta tgattgaaga gtggggtgag gataatccat tttatggatt
    28021 gaagaatacc ggacagcttg tggaacaatg gacaaacctg gatggattgg agatattcta
    28081 tagttatgtg cgcaatagca aatgggttac cgtgacggtg ttacctactg aaacaggcat
    28141 acatcctgta agcaatagtg tttataaatg gaaaggatat ataaatgaat acattgctga
    28201 aactgctgtg tggtgggcat ttgaactact gactgaaatg gaagcaaaaa aattcatgat
    28261 tcaacataaa cctgaggtaa agtttacttt tatacggttg ggacatccct atgaattggt
    28321 tgtaaaattt gacggttatt attgggttgt tgaagattga tacaattcta tgattcttta
    28381 tgcctgtcac agtaatctca tctatagcct ttaaaagaac ttttgtatag tatcggtata
    28441 tactgtagcg ttcatttgca tttcaacaag ccaagagtcc accatatcat tttgcatgac
    28501 caaagaattc atttgttatt tcgataaact caccatccac catgtcattt cgacgaacga
    28561 agtgaggaga aatcttgaaa tgcacgcaaa agatttctcg ctgtcgctcg aaatgacata
    28621 gaagaggagt actcgaaatg acagaagtgg agcactcgaa atgacacaag gggagccgaa
    28681 ataactggtc gctttgacac gcgcacggtc gtccattagc tctgccttga tacgtgcatt
    28741 gcgtgcgctt gtgacaacgg aaggcgtatc gagacgtggt cgtcgagtgt tccgaacgca
    28801 gtgaggaatg tatcgagacg accgcattgt accaaatgag tggattatga taatataatc
    28861 tttatagaat tctcttaacg accatttttt aagacagctc cttattgtta ttactatact
    28921 gctaattgtt tgttagttcg tgtccatatc ttcatctttt ctgcctgttt taccaggtca
    28981 tcctgaaatt ttggatgtgc tatgcttata agtttttcgg cacgcatcca ggttgggcag
    29041 gcaataagtt taacggcacc aaattcagtc acaatgtagt caaccatctg gcgggggatt
    29101 gtaactgttg aagcaagtgg aagctgaggg actattcgtg actgaagctc acctttggaa
    29161 tctgtgtatg ttgatgatag acatataaag cttttgccgc gatttgacca ctgcgaacca
    29221 aggacaaaat cccacatgcc accgttacca ctaacctgtc ctgaaccaag gctttcagca
    29281 tttacctgtg aaaacaggtc aacctgtatt gcattgcata tacttaccat gtcatcattc
    29341 tgggctatga tgcgcgggtc attggtataa tcaacaggat acgaagccaa tgcctgattg
    29401 ttgtgcataa aatcgtacat gcgctgtgat cctattgcaa atgtatatac acatttgaat
    29461 ttatcgatgt ttttcttagc accattcatt cgccctgatt caatcatgtc aacgtaggca
    29521 tcaacaaaca tctctgtgtg gccacctaaa ttctttaaat cagactgagc aatgagtttt
    29581 cctaccatat tgggcatacc gccaatgcct aactgtatgc aacatccatc atagagaaat
    29641 ttcattacat gttctgctat ttttttatcg gtttcggttg gtgggacgtc gggtgctgca
    29701 aaaagcatct ggttttcagg agcttccaca atgaaatcaa cacgagagat gtgaattgct
    29761 tcttccgaac cacctaaaca gcgtggcata tttttattca cttcaacgag cacataatca
    29821 gcggcatcaa cttttgccga cgtttcggaa ttctgtggtc ctatattaaa aaatccctga
    29881 tcatccattg ggccaacctg caacatggca atccatttta catgtttact cttttcagga
    29941 cgattgtagt agaacgaacg atatcctgga tcaatgtttt catgcggatc ctgatttaat
    30001 agatagcgat aatagaatgg tgccctgtgg tacagtattg ggcaataata agcagcttca
    30061 acctgtgtgt gaagcagacg agttagtttg ctccattgcc agtcaatata cgtaaaactt
    30121 tccgggtgtt tgatgacctc tggaactgga ggcaatgtaa ctgcagtata gattaataca
    30181 tcctttaatt ccggggctct acgtgccagt gcctgatcaa gaacaacagg tttgccattg
    30241 aaaaaaccat aatcaacaac atcaccacat tcaacaactt tgactgcatc atcgggtgtt
    30301 gttaatttag ctttgtattc attccagtaa ttcataagtt tcctccatga tagaaaaata
    30361 atctcattaa aaatattata ataatacaat cttataatgt caataaaaat atttattata
    30421 attaataatt tttttagata gttaataata tatatacata ttataaataa tgtaatatca
    30481 gaacaaaata atgagaggta ttgagtgatg aagttcaata tttatactat atgatagggg
    30541 taatttataa ctttactttc ttatttatat atattacatg atgtatgtgt tgttattcat
    30601 ttactgatta cgttaccatt atataatcat aaaaagctgt cattatgtat agaagcgttg
    30661 taatcattga gaacattgta gaaatggcat tgaagtgata caatgattaa ctattgtttt
    30721 cagaggaggt agccatgacg cctacaatac aaaaaggctt tatagctgtt atgattgttc
    30781 tggtgatttg ttttggtata acaactgcat atgttttaaa taccaatgag attaagctta
    30841 cgatagatta cgataataac acaccatata ccgaactatc attgcaattt tccagagata
    30901 tatttatagt ttcagatatt gatgcaatga aaaatgatat agctcaaaag ctcactgtat
    30961 catttaatcc tgagcttgaa tatatattaa aacctttgga ttcacgtaat tgcattcttg
    31021 aaatatataa tcccaaacca gcacatgaat accagtttac cattattgta aaaaatattg
    31081 ataccaaggg gttatatgta acagcaaaca ataaagaaat tgagttaggg aaaacctact
    31141 ttttcagaac tcctgtgctg cgggtttatc attgttccaa agaaagcaaa atgataaaca
    31201 gcccaattgt gctgcaattt aatttccctg tttcactgaa tgttctcatg caaaaaatgt
    31261 caatacgtcc ggatgttcag tatacactgg aatacgatag taatgatacc agccataaaa
    31321 cagtaatagt gaagcccatc aatgaaaaga aagcacaaaa atattcattg accattgata
    31381 aaaccatggt gccagccagt ggtgtcattg gcatggatac ttcctataca tttacctata
    31441 caacctattt cccgtttgag ctaaccgata tacaatcatc atacagtcgt tactatgatg
    31501 attcatcata ttatcctgat tatcctgttg aatttacttt caacaatgaa attgctcttc
    31561 ctcaagggaa aagtgttagc gaatttgtaa ctgtgacacc tgatccaggt ggtttgcagg
    31621 tgaatatgta tggtaacaca attgtggtga gtggaaattt tactggcaaa aaaacataca
    31681 cagtaactat cgcacaaaca ttacaggata tctatggaca aacattacag gaacctgttg
    31741 aaacggaaat tgaatttgag catgcatttt cgtatttttc atgtccaaca ggatatatgc
    31801 tgctggagaa ctatcttgag aagattattc ctttacgcat ggtcaatgtt tcatcatttg
    31861 aatgcaggta tctatcgctt gtaaatgaaa acgatattaa agcatacttt gaaagcccaa
    31921 aagaatattt taataaacat gcaaaaacga aaacgtatat actaaactgg acatgggatg
    31981 tgtataaaac tgttaagttt gattattcaa aactgcatca gggagataat gggatttttg
    32041 tttatgagct aaagccagtt atggaacatc cgtatgaaaa taatcatgat gtctatcgtg
    32101 gcatgatgca actttcagat attggtgtga ccattaaaaa aacaaaaaat gatatattag
    32161 tacatgtcag agatttattg aacaacaagg cattagccgg tgcgtcacta ttctgtactg
    32221 gggcggaagt gtcgctggga caaacagatg ctaaaggtgt tttgattgtt aaaaataatg
    32281 gatgttcgct gtttacgata aaacatggca aaagcatttt ttattactca atgcagcgcg
    32341 ataatggcta tagcgatgaa gatgaacgtg ccaaaattaa tcttccagaa aaatattatg
    32401 gcagtgatta ttacatacta aagcctcagg tgcatctttt taccgacaga tatttataca
    32461 ggccaggtga aacagttcat gtcaaaggaa tactcaggta caggaataat gaccagtgga
    32521 gtattaaacc accgctgtca gtaataaaaa atgttatact caccgtcatt aattcgcgcg
    32581 atgaggagat tgcttcttct gccaggtcag tgtcagcaac cggtggcatt gagtgggata
    32641 tagcacttaa agaagattat cccaccgggt actatcgcat aactgttagt tccagtaata
    32701 aagattatga tcttcatcaa agtattgatt tccaggttga ggagtttaaa ccggcaaaag
    32761 ctgaaatgaa aattattcca tcaaagctta cgtttttgtg gggggaaatt ctttcggttg
    32821 acctgtttgg atggtattta tttggtgccc cggtaaataa cgatgtggaa tatacggtta
    32881 cattgcagcc ggtaacctat cgcagtagcg tatttgctga ttattcattt tcaacacagc
    32941 ttaattatta tgatgaatat gatgagtata gtccaccatc aacggttgtt gccagtggca
    33001 aagtaaaagt aaatgaaaaa ggcattgcaa aggtgcatgt tccgcttaaa gctattgcca
    33061 atgtgcccaa tgcagaaata ttgattactg gacggacgta tctttcagat gattcgccag
    33121 tatttggtgc aaagagtgga cttacggtgg cagaaccggt tcatgttggc ataaaggttc
    33181 cgcgctattt taattataca aaagagcctg ttaccataca gcttgttgcc tgcaacaatg
    33241 atgatactgt tgttgaaggt actccaataa aattagttat aaaaaaacat gaatggaaat
    33301 catttcagat agcgggagta aatggaagat tgcaatggga atacaaaaaa atagaaaccg
    33361 aagtcaatct acagaatcat actattggga aaaaagatgt agtagcaact attaatgatc
    33421 ctgggtacta tacagcagaa gcatatgaca gccagggaaa aagaaaatat gcagtgtgtg
    33481 atttttacgt tctgggcaaa ggcgaagcag ggtggcgtgt gaacgatgat gaaactgttg
    33541 atgttgaaac tgataaaaaa agctacaatg ttggtgaaac agcaatgctt ctggttaaga
    33601 atccttttac caaagctcag gcatttgtta gtattgaaag agaaaaattt tatgattact
    33661 ttgaagtgcc tgcaacatca agtgttgtta tgattccggt caaaataaca aaagagcata
    33721 ttccaaatat ttatgtaagt gttatgctgt attctggaag aacagggttt aatgccttga
    33781 aagatgggga ggatagggca aaaccacgct gtttaattgg atacgccaga attaaagtta
    33841 caccaaaaga aaagaaatta tctgttcagg ttaaaccaga taaaaagcaa tatgagccgg
    33901 gtggctatac aacggtggaa ttgctggtaa aagattataa aggacaaccg gtggatggag
    33961 aggcaacaat atctattgct gatagaggtg tattaaatct tgtcaattat acattacctg
    34021 atccctatga atacttttat gccccgcgac aacttgcagt tactaccttt gatgtacatc
    34081 agattattct tgggcagcgg tatcttaaag aaaagggcga actgataggt ggtgatggtg
    34141 gcattgcttc aatgggtatg attgtcccac gggctgatat acggttcagt gcatattata
    34201 aggcaacagt tgtggtaaaa cagggaaaag caaaggtgca atgtaagctg cctgataacc
    34261 tcaccagctt caaatcgatg gttgttgttc acacagaaga ttcaaaattt ggatatggtg
    34321 aagcaacctt tgaggttaaa aaacctttaa tgctattgcc aaccttcccg cgctttgttc
    34381 gtgttggtga tacgtttaat gctggtgctc ttgttttcaa ttacactggc aaacagggaa
    34441 acgttacaat aggcctggaa ctgaaaaatg gcttgaagct taaagatggt tctgcggttg
    34501 ctgtcaaaga gcttattctt aataatgggc aatctcagga ggtaaccttc ccggttacag
    34561 taactggaca agcaggtatg gcaggtttta cggtcaaagc acaggcagga acctatacag
    34621 atggcattac agataccata cctatcaaag ctcctaatgt atttgaaaca gtggcacagt
    34681 atggaaggat ttcagctcca cagtccagag aagtacagga gataaccata accagaaata
    34741 ttatacctga agtctcctcg attaatattt ttacttctcc gtcggcattt gtggatctta
    34801 aaggttgcct tgattattta gttgactatc cctatggatg ttttgaacaa aaggcttcaa
    34861 aaattttacc actcattttg ggtcaggata tcattgttca aaaagggctt ttaaaaacaa
    34921 agtcaaagga tgaactcaga aaagtagtta ctgatgtttt gaaagaattt cctgcttact
    34981 acaaaaatgg aggattttca tactggaaag atggttcgct tcccagtagc tatctcacag
    35041 tatatgctgc atttattatg accatggcca aaaaaagagg ctatgatgtt gacaatgcaa
    35101 tctataatga taccattaag gtgataaaac agtttgtaaa aaaaggtaac atatttagcc
    35161 atgagggaag gaactcatcc tggtatacct atactacact tgcttttgct cattatgttg
    35221 cagcattgca aggctttatc agtgaaaatg aattaaagct tgtatacagt acaataacca
    35281 aaaaatatga tgattgcatt gccgcatatg cttatctact gaaagccctt gcattatatc
    35341 ctgattcaac attaaagaaa acactggtaa aagaaattgc tgaaaaattc ttttcccgca
    35401 tgcggactga gtcgggatat gtgtatttta ctgatacatc gcaatggggt tatttttatt
    35461 atgatacggt cctgtcaact gctctcattt tacaggctct actggaagcc aatatcccct
    35521 ttaataatga ttacaagatt gtgcagtatc tgctgaaaca gcgcaaaggg tatgcatggc
    35581 tcaataccca tcagaatgca gtagtatggt gggctctcaa tagctatctt gtacgctatg
    35641 aaaaagataa acctgattat tcacttcagg tgcttgttga tacaaaagaa ttgctgaatg
    35701 caattgttaa tgaaagcaat gtgcattttg caaaacaata tatcgtaaca tcagaagata
    35761 gtggtgtaaa aaaattcagt tttattaaaa atggaaaggg tgcctgttac tttaccatgc
    35821 aataccaata tattcctgta tatgaagcaa tgacgtcaaa aaatatgggg tttgaggtaa
    35881 gcaaactatt taaagatata aaaacaaaag aaactgtcaa aacattcaag aaaggctcag
    35941 agtatattgt tgaaataacg gtatatactc ctaaggatcg tgggattact gtgcttgaag
    36001 accagttgcc ttcaggattt gaacctgtca atgtgacatt tgccactgaa gaaaaggtag
    36061 cagtagacaa aaagagtgat tacacaaact ggggtacgtt caatcattat gagcagtaca
    36121 gtgacaggat tatctattat gctgatttta tacagcgtgg aacacataaa gttatattca
    36181 aggtgagagc aacaaactct ggcacattca aactccctgc ctgtaaggtt gaagagatgt
    36241 acaaccctga agtgtttggt atactgtact tcagcaatac tgttactgta gagtagctat
    36301 gaagcgctat aattggaaac atattgactg taccagcgcc aacgttttaa aagcaagttt
    36361 ggttgttttt gtagtggcga tagttaccta tataatattt ccatacaagg atggatacct
    36421 taccgcaaac aataaatctc cgattactat ttacgataga catggcctgg ttttgatgga
    36481 aattactgat gggaagaaag gactatctca gtctgttaaa atacaggagg tcccatctga
    36541 atttatagat ctgctgttat tttctgaaga cagaaatttt tatgatcatt ggggtatatc
    36601 tttaaaaggg attgcacggg cgctgtatca aaatctacag gttatgcaca tagtatctgg
    36661 tgggtcaacc attacccagc agcttgttaa atcaaaaaaa gagattatgc gcaacactgt
    36721 agtaagcaag ttggcagaaa ttattgaagc tatacggtta gaatgccatt ttagtaagaa
    36781 agaaatatta caggcatata ttaatgaagt ttacatggga aataacatat atggttttga
    36841 aaaagctgca caggtttatt ttgacaagca tttataccag tgcgaccttt tagaacaggc
    36901 atttttaatt tctctagtta aagctccaga aaggtataat ccatataaaa accctgagac
    36961 aattgtcaaa cgggcacgaa tgctacttga taaagcatca cgcaatgtta ttaaacttac
    37021 tcccctacag caggaagctt acaggaataa aaagattcac ttgcagtatg atgagaatac
    37081 tatatgtgca cctcttttct gtttatatgc attatcaaag gctcgagaat tattccctgg
    37141 taaagatatt acaaaaatat atacaacact ggatgcagcg ctatataaag acttactggc
    37201 tgtggtacgc aactcattga gtacattaaa agataaaaat gcactccatg ctgcaatcgt
    37261 catgattgac aataaaacca tggaaatcat ggccatgatt ggcagtgtgg atttctttga
    37321 ttatggaaag ggtcaggttg atgcaacact ggttaaaaag caggtggctt caaccatgaa
    37381 gccatttgct tatgcattgg cgctggataa agggatattc catacatcaa gcatcttgcc
    37441 tgatgtatat actcagtttt attcaaaggt tggcaattat atcccaaaaa attttagcca
    37501 gagttatcat ggtccagttc gattggctaa agctttagga tgttcgtata atataccagc
    37561 agtatatgta ataaatactg tagggatggt acctttttat aattttattc gttcaatagg
    37621 ttttgattca attaaccgat ctccatcatt ttatggtttg gggatagttt taggtaatgc
    37681 tgaagttaca ttgttagaac tggcaaatgc ctatacaata tttccacgag aaggtatatt
    37741 tgaacatgcc aattgtatac gtaaaattgt tgacagagaa ggaaatgagt atgtggtaac
    37801 acctgataaa ggaatcagag tgttacaacc atcaacatgt tatcttatca atcatatact
    37861 ttcagaatat aaatataagg ttgaggcttt tggattacag tcagcaattc attttccctt
    37921 tccctttgcc gcaaaaactg gtacatcaaa ggattttaaa gacaattttg tagctgggta
    37981 taataaaaac atcacctgtg ctgtatgggt tggtaatttg tataatgaaa cgctggataa
    38041 tcttcccgct gtatctggtg caggtattgt actaaaaaat atccttcttc atttgtggaa
    38101 tagtggcttc ccctttgcaa aatttacatt tcccacaaaa gaagttcatg aggtcaggat
    38161 atgtacactt tcggggttgc cggcaacccc tttttgccct caaacagaat atgagttata
    38221 tgcacatact gtacccaaag gtgtatgtac atggcattcc agcggtaaaa caacagtccc
    38281 ggcagaatat gttcggtggg ctgcagaaaa acattacagt gttttaaaag attctgctgt
    38341 aaggattatt tttccaaaaa atggggcaac gttcaaaatt gacaaaactg tgcgaaaatc
    38401 cctgcaggca atcccattac aggcggcagg tactaaaaaa gaaatacagt ggtttgttga
    38461 tggtcatctt attgggaagg gtagtgaaat agtatggcag ttacaacagg gaaatcatat
    38521 aatatgtgcc agatcagata aagttgcaga gtatgttaat atagtagtat tagaataaaa
    38581 aattataaaa ttaattgtca ttattcaaat tgtctactat ggtgtaaaaa aaatttaaaa
    38641 aaataatgga gtgatactat gatgcacaag ttgttttatc ctgattcagt tgctattgtt
    38701 ggattatctt cacgacaaaa caatatacca cgtcttacac ttgaaaatat gctgcgctgg
    38761 ggatatcggg gacgcatttt tggcgtaaat ccaaagagtg aagatgaaca ggtagatggt
    38821 atacgtatgt ataaatctat agaagctttg ccagaagttc ccgatttagt ttatgcactg
    38881 gtaccggcaa agtttgttcc tggaattgtt gaagaatgcg gtcaaaaggg ggttaaatgg
    38941 atggcaatcc ccagcggtgg tttttcggaa tacagtgaag aaggggaaaa attagctatc
    39001 caaactgttg ctacagcaaa gaaatacggc atacattttg ttgggcctaa tggattaaca
    39061 gtagccaatg tagaaaatgg tttgtgcttg ccatttgttc ctttacttcg ccctgagttt
    39121 ggtgggatgt caataatttc gcaaagtggt ggtgttgcca tcatgatgtg gaatattatt
    39181 atggatgaaa atattggcat agcaaagttt gcaagtattg gtaacaagct tgatttagat
    39241 gaggttgatt ttttagaata tttaggcaat gatcctgcaa catcaattat ctgtatgtat
    39301 ttagaaagtg tgccacgggg agctgattta ataaaagttg ctgaaaagat caataaacct
    39361 attgttgtat ataaatctaa taccacacag gcagggaaaa aggctgccat gagtcatacg
    39421 gctgccatga gcaatgatga agaaatactc aatgcagcat ttgaaaaggc tggaattatc
    39481 cgaatataca attatcatga cttctttgca gtagcaaaag cattcaaact tccacctatg
    39541 cgtggcaagc gtataatggt tatgagtcct gccggcggtt ttgctgttat gactgccgac
    39601 ctgtgtgaac aggcaggttt tgaatttgca gatcccggtg aagaatttta tcaaagcctt
    39661 caaaaatttt caaatgcagg tgtaatccac ttttcaaatc cactggattt aggtgacatc
    39721 tatgacccgg catttactgc ccatgttgtg tattcagtaa tgcacaatca aaatgtggat
    39781 ggcgcggtat atgtaagcca gcgtccacag atgcctgata gagaaagtgt tttttcacga
    39841 atgtttttag ctgacctttc aaaggaaacc tggggtgcca ttgtatcatc agctaaacct
    39901 ctgggtattt gtttacttgg tttatctcgc gtgatggcac agataaagcg tacggtaaat
    39961 ttccctatat ttaattcacc tgaagaaatg gtgcgcgcac ttgcatatca gatgaaattt
    40021 aatacacgac aactcaggca aaagcaggat gaaccatgca ctgtgtcgtt tgacggtgca
    40081 atgcagtgga ttaaacgcaa taccggtgaa attggtgaag atgcaatgga actgcttaca
    40141 acaatagatg taccggtacc gccatcaggt attgccacaa cggaaaaaga agcagtggcg
    40201 atagcaactc gtattggcta tccggtggta tgtaaacttg tatcaaaaga gatattgcac
    40261 aaatcggatg ttggcggtgt gattgttaat gtaaccagtg atgatgcggt gaagcaggct
    40321 tttgcaacga ttcatgcaaa tgtactatcg cacaaacatg atgcaacaat tgaaggagta
    40381 cggatttcaa aaatggctgc aaatggcgta gatatgtttg ttggcagtaa atatgatgat
    40441 tcgtttggcc aggtggtact gtatgggttt ggcggaatct atgttgaagt atttaaggat
    40501 gttgcctgct cgctgtgtcc tgcaccaaaa gatgaaataa aagaaaagat ttcaaagctt
    40561 aaatcgtatg cattattaaa aggtgcccgt ggccaggaac caaaggacat tgatgcgtat
    40621 gtggatataa tctggaaact ttcacatctt ctggcaaggg cgccacaaat aaaagaaatg
    40681 gacttaaatc cagtacgtgt atttgataat ggctgtcagg tgcttgatat caggataaag
    40741 attgtgcaat aattgaaata accattgtat ggtgctacgc tttacgtatg atttatttag
    40801 ataatgccgc taccagttac cctaaacctc agtctgtaat aaaagctgta gctgactgtt
    40861 tgcaggatta ttgtgcaaat cccgggcgtt cagggcacaa acttgcaata gaggctgcac
    40921 gaaaggtgtt tgaagcgcgg gagacagttg cacagttttt taatgttaca gattcccgta
    40981 acgttatttt cacttcaaat gcaaccatgg ccataaattg tgcatgtgct gcaattctgg
    41041 aagatagtga ccatgtagta gtttcatcta tggaacacaa tgcggtgatg cgaccattgc
    41101 ggtatctgga aaaggaagga aaaatatcat tatcttttat taaatctggt agcaacggaa
    41161 aaattacagg tgatgttact caattattac aacccaacac caggcttgta gtcataaatc
    41221 atgtatccaa tgtcaatgga gtagtacagg atattgctca catcacagca aagattaaag
    41281 catatagtcc ttcagttacg gtacttattg atgcatcgca gagtgccgga gtactgccaa
    41341 ttgatataca gaagattgga tgtgactttt tagcgttcac ggggcacaaa gcgttgtatg
    41401 ggccacaggg gattggcggc ttaattatta atacagatag gccattaaaa ccttttatta
    41461 tgggaggcac aggaagcaaa tccgaaagcc agtatcagcc tgaatttttg cctgataaat
    41521 atgaaagtgg tacactcaac cttcccggta ttgtgggatt gaaggctgga atagaatatg
    41581 tttggccaca aatggaaaag atacgtactg aagaaatgaa acatatcaaa aaagtaatag
    41641 agtgtttgtc tggaattaaa ggtataactg tatattggag taatactttt gaacatcaaa
    41701 ttggcgttgt atctttttcc catgaatcaa tttcgtgcag tgaaataggt cagcgtcttg
    41761 atgatgaata ttccatttgt gttcgtattg gattgcactg tgcacctctt gcgcatgaaa
    41821 ctataggcag ttatccagat ggcaccgtaa ggatatctgt tggcacattt acagaggata
    41881 gggatatcga tacattttgc agggctatcg aacaaataac aaaggtataa aaaatgaact
    41941 attacctgtt attattgcca tctgtacatg atgtgatgaa ggctgaaaag attttactta
    42001 atgcaggtgt taatgtgaat gtaatagcca ccccacgaga aatatcgcat aactgtgggg
    42061 tggtaatacg ctttgaatcc agtaatttac ataagctgtt agtccttctt gaagaactgc
    42121 ctttggaaaa aaaggtattt aaaaaagttg atgataatgt gtacgttgaa gttactcaag
    42181 agtagattct aactttttga tgctctcatc aagtattttg tattccttgg aattttttgg
    42241 aaatgtactc tggtactttt taaagtactt tatctgttcc aatttccagc gcttcatgtc
    42301 aaaattattc tggatcatat taattcctcc gtttaataaa ctgtcgtact ataattttcg
    42361 taaaaaaagt aaaataattt cattatttta ttgtttgaac tattgttatt tgcaaattta
    42421 atgattattt aatatgtaac aatatattag aatataatac ttgttatcgc cagtaagtta
    42481 gtatatatta aaatcacgga atttgttacg taaaggaaat ttttatggaa ggtgccgtta
    42541 gtcaatagta cctgcattag cttgtagata gaaagaggta agaaaaaaaa gtatgaaatt
    42601 ttttgtcccc ttaagataac aggtgatggt aaggtaactg taacaaagac gtgatggaat
    42661 gataaataaa tttttgttga taatggttgt atgtaatgac gatagtataa tgataagcta
    42721 taataaaatt atttaagatt attttaaatt ggtacttagt aggaagctat gattaacagg
    42781 gatagtattg acataaaaga tttaccagtt tttccgcagg ttgcaattaa gattttacag
    42841 atacaggaag ataatattga catcagcttc aaggaactgg aaagcatcat tttacttgat
    42901 ccagcattaa cagccaaaat actgaaagtg gccaattcag cactctatgc gcgtcagcga
    42961 gagatcacca atttgcaaca ggccttgacg ttacttggtt tcaaaatggt aaaaagcctt
    43021 gtgttactgg tttcagcttc aaatgtttac agtaaaaatg taaaatcgca acagcagtca
    43081 accgctgcaa ctgttaccaa aacatcaaca gcaatgatct ggcggcattc agttgttact
    43141 gcattccttt caaaatatgt ggccacacgc attaaaaatg atgaaaaaaa agaggatgta
    43201 tttgttgctg gccttcttca tgatattggc aggcttatca tgatgataca cttcagggat
    43261 aaatatgagc aatacattgc atatctcaac caaatgcagt ataaggatat acgcgaagta
    43321 gaagaaaaaa tatttgagat aaaccaccag gatgtaggga agatagttct tgagaaatgg
    43381 aatttcccca atgaactgat tgatgctgtg gcacagcatc atgtaataca ggttgactca
    43441 aaatataaga ccactgttca gatagtaggg ctggcaaatc tgtttgcaaa aattattgaa
    43501 aatgaaaatc tttcagaaaa tgacagggaa ttacttaacg ccttcagcaa ggcattggnn
    43561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    43621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga aaatctttca gaaaatgaca
    43681 gggaattact taacgccttc agcaaggcat tgggtatcac tcaggaagat tataactatc
    43741 tgacaggaga gattatcaat acactgcaga atgatgaatt atataaaatg agcacttcac
    43801 tgattggata aacttatttt atgtcctgct aaaaataata aaatcaatta taggtgcttt
    43861 tttatacaga tgatagccac agtatagtgt tgttgctacc gatgccagca tatagctggt
    43921 acctggaagt aacgggaagt atagtattga aaataacgtt gctgcaatgt ttaaaataag
    43981 atacagacat gcagccattg cagcttcaaa ttgcaattcc atatacaaaa gataaatcat
    44041 aagattaagc gtcaacatat gaaagaatac agctataagc agaattctca taatgtgaat
    44101 attaattgta tgcaaatacc ccaaaaattc cagtatctct ggtagcttta ttaaaatacc
    44161 aacggtccat attgactgga aaaatataat acccttaata ccatttttaa gtgataacag
    44221 catcttttgt ttttttagct gtatatagcg gaatgggtct tccagtattt gtgaaataaa
    44281 tgcaaagaat tttttatgaa aatctgtttc agcaataatt aaaaaatata ccagtccggg
    44341 tatcatagtc aggtatgcca aaaacaccgg tatatcataa gtgatgtaat agtaatacag
    44401 tgtaccagga atatgctgtc cctgtaaaaa ccagtaaagc attttatccg accatatggc
    44461 catattaaag aagcatccca ttaaaaaaat tcgtttatgt tctttaaacg atgcaataaa
    44521 ttctttattt agtgtaaatt ttttaagggg gtatgtaatc tgggaaatag ctattaaaag
    44581 caaaacaatc acagcctgac caattgtgta cccgcacaat gcacctgcaa cgccatacaa
    44641 ttttcctaaa taaaggacgc cataaatact ggcgatagtt cctgctaaat agctgaagaa
    44701 aattttgtaa tatgctttga gcagggcaat atataccagc atgatccaca ggatgttaat
    44761 cacaacaaaa agtgaaacta acgatagtat ataaagagtt tgaaattgaa tgaatgagaa
    44821 atcattaaaa cacacaaatg caagagcagg cagtattgac agtatggtta taataattat
    44881 tgctgttaac agggctgtgg ggatggtttc ataattttca atatacagtt gatcagcaat
    44941 atagcgtgag aatacataat gaaaaccacc tgataaaaat agtgaaaaag catacacata
    45001 cactatcgaa acagtaaaaa gtgcaggatt gttagcaatt gccccaaatg tatatgtttg
    45061 aataatatat atagtaagta ctgacaatat ccatggtcct gcaacaatga tcatgcccag
    45121 aaagaaagcc tgtacaattg atgacaacgt tcctttgtgt aaaattttat ataattcaaa
    45181 tcctatcccg gccatacatc ctcattgtgt atacatgcca atatattttg cataaagatt
    45241 gtcatatgtt gcaataaggt cttttttatt ataaaactgt ataaccttat tcctgtttgc
    45301 agctatcagt ttattacggt aatcaggatt attgtataga taaataacac cctgggctaa
    45361 tttttctgaa tctttggggt cggctaaaag aatatcattg taatcaagca tttcaggaac
    45421 attgcccacg cgggttgaga ccacaggtat tcctgctgca taggcttcta agataaccag
    45481 tggttgcgct tcccggatac tggaaagtac caccacatct aaaaatttgt aatactggcg
    45541 cacatcagct ttaccggtga atactacttt atcctgtatc tggaaatttt caacaagctt
    45601 aacgcaatct tcataataac tttcgtcttc atcagttggg ccaataatgt aaaatttaac
    45661 atcagggatt gattctgcaa caatctttgc catgatgata aaattcttaa catcttttat
    45721 gggaacaact cgcgccacaa tcccaacact gaattccttg cgggtttctg tttttagatc
    45781 taaatatcga tctatttcaa tgccattggg tataacaagt gttttttcgc gctttgcccc
    45841 ctgtgcaatc tgaatattgc gattaacctc aaaaagagat ataacacaat cagagtattc
    45901 atacgtaata gcgcttaaat cattaaatat gttaatccac atatctcgct ggtagccttt
    45961 aacccacgtt gcgcgtttta tgtctatctc acgttctttg ttgtataaac catgctcagt
    46021 caggattaca ggtttattgt atttaatctt tgcactggca gcacatagcc ctgcatatcc
    46081 tgttgatatg gtatggtaca ggtcagccgg tggcaggtcc caggtcataa tcttcatcat
    46141 gtattcatgt gatgatcgac atgcccagta atagtcagaa aagggatata agggatttcg
    46201 tatttcatta tactgagtta caatgttaaa acttgtaata tgtcgctgca gagaagatgg
    46261 atcgtagcgg tgcttattaa taagcttaaa aatttcactg aatcgagaaa aatctttttt
    46321 ttgcatcata tcattgtgaa aggattcaat attgctgaaa aacgatgact gtattgactt
    46381 tttgggtttt tcaactgggg ctgtcagtag cctgttaacc agtccaatca cgttgggtgg
    46441 aaataaatat tttggttcgt cctcttctgc tgatattgca aatatgataa agttatagtt
    46501 ggtaagccct gtaattatat cctgtatcca ggctgatacc cctcctgtta tgtatggata
    46561 tgacccttct gcgataatac atattgtttt ttttgatgga tcaggtttat tgattgctac
    46621 aaaatccatt gatagattaa cacctcggca aattctatat cgcttacatc gtactgagaa
    46681 ataattgaag caattgtttt aattctgtca taatgtttta aattataatg gcattgagcc
    46741 attttgagca ggcaatatac aggtttgtat tgtatagaat aacaatacgc atagagtgat
    46801 agtgcatatt caaaattttc atccagtaag tagctgtctg ccagagctat ttttatcctt
    46861 ttgttttttg gtcgtaatgc atatgctttt accaggtttc cgattgcctg tttaatataa
    46921 aatttctgga ttagcttctg gctttctaaa agtgcagcaa attgtgtata aagaaggcca
    46981 agcaaataat ggtaaataaa gatattatgg gtagcttttt ccagcttttt ctttattttc
    47041 tcaatttttg ttaaaaatga attttcaatt gtgtttaatc cttcagcagc atacagggct
    47101 atttctggat tgggatcttt aatcatggtt cgtaatattt ttactgagtg ggggttaggg
    47161 cgcatggcta atttctgctg ggaaaataac cgtgtaattt cgtccaggtc ctgggcttca
    47221 tcaattaatg gtcgtaatga agtagctgcc ataaggtaat gttctatacc cagcaagcct
    47281 tcagaaaaag ccttacgcaa tttatgctga gttttaacaa tataaggatt ttcaacaaca
    47341 tcatgcctga aaaaatcaaa aggattttca aaggaataca gcttaattat atttttgctg
    47401 ctcttagata cagtatagtc aaaaataaaa tcttcatgag gaagaatggt gctcatgctc
    47461 tcacctatat tttctgcatt aacagtagat tttcggccag tagtataagg tcttcttcag
    47521 attgatggtt gggtcgcaag ctgctgtatc caatgactat ttcagggtta actatctggc
    47581 cttttatata atatgctttt ttactgtttt gttcgattag cgataaacta aacattgctg
    47641 caccatcaaa atccatgttg ggtaaaatag ctgcaaactg gaatgtttcc ttatattgaa
    47701 ataataatac ttttcccttt gccagttgtt gaactaactg agctatctct tttaatacaa
    47761 ggagtgcatc cttcattgaa aacttttcaa ctatttcagc gctattagta cattcaacta
    47821 tcagtaacga caaattcaat ttattatgct gagcgttagc aaattcttcc tttaatacct
    47881 cataaaattc actgattgaa ttaaaaccgg ttacggggtc aacctcttta tgtaccagtg
    47941 agctatacct gatagcatta cggataattg gagctgccag gtcagcaatc atgagcagta
    48001 actgttcagt atagagatta tattttataa aagggatttt ttcaatgttg agcgccccca
    48061 tacctggtta tcaatagtaa tgggaacagt aataatggaa ttgccattat caagtgcctt
    48121 caagttttga tatttctgga gcattttaat ggaaaacatg gtattgtttc gtaccgacca
    48181 gccaataagg ttttccgtat caaggtttga aggggtacta tcaatgatgt cctgtgtcca
    48241 cccattatat gcaagcagat gaatagagtt attatcacgg tcatattgcc acaatgagca
    48301 ttgctgagaa ccggtaaatt gaacaacggc attcaagata ttaggataaa tattttctaa
    48361 atgtaatgta tttaaggcaa taatggttga atacaatgag ataagtgaat tttgctggcc
    48421 taaaatacgg tcctgatatt cctgattaac cattgaaacg gcttcaagtt ctcttttaag
    48481 cttggcagcc tgctcatcaa gttctttgat aatatttttc tggttttgaa taatgcggcc
    48541 taaattgtca cgtatttcgc caagaataat aatagcaata agcgatagaa ataataatga
    48601 cgatagatgc tggtaactaa ccggtgtggt gatagttgta tacagtatgt ctgttgaata
    48661 atacagatca ctgattataa tatatgatgc tatgattatc gttactgtaa ggaatgtaat
    48721 aagacctgat tttttgccat aaaacgaagt aaagaagatt ataacaaata gatatggatt
    48781 gtagtgtata gaaagaaaac caatatcgtc aggatatttg agaatattga agatgaatac
    48841 tgctgttaca agaagaatga tttcaagtgg aaatattttt ttaaatattt ttactatgat
    48901 atccataaga aacagtatat ttgtgttaag ttataataaa gtgtattgtt caatactaat
    48961 aacaaaattt agtaaatgtc aaggaaatta gaaagtataa attttgtaaa gtaataaaaa
    49021 aggccgttcg taacgaacag ccttttttat ttacatggta ttatatgtta ccacttttca
    49081 actaattcaa acagtgcagc agcgcccata ccgccaccaa tacacattga ctcaataccg
    49141 tatttcacac ctttcttaac catcatgtta acaagctgtg aagctaattt ggaaccagta
    49201 catcctaatg gatgccctaa tgcaattgct ccaccgttaa cattgatgcg gcggtctttg
    49261 ctgtcaactg cccactcttt cttgtcgcca atacctaatt gacgggcgca gtaaatagcc
    49321 tgagaagcaa aagcttcgtt gatttcaaac aggccaatat ctttcatatc tttgccagtc
    49381 agtttaagaa gttttggtat tgctactgca ggtccaatac ccatttcatc aggctcacat
    49441 cctgcaacaa cgtaggcaac caattttacc agtggtttca cgccatattt tctgcatgct
    49501 tcttcgctca ttaaaagaac ggccgctgca ccatcagttg tctgtgaaga gttacctgct
    49561 gttactgaac caaatgctgc aaaggctggt tttaattttg caaggccttc gattgttgtg
    49621 tcagctctta ctccgtcatc aaagtcctgt ataaattctt cttttttgag tgttccatct
    49681 ggctgtggca cataacgaac agcttttgta ggaactatct catcaaacat cttgtttttc
    49741 tgtgcttcag cagctttcat ctgtgagtga tatgcaaatt cgtcctgatc ctgtcgggaa
    49801 attttgtaac gatttgcaac attctcagca gtgataccca ttgagatata tacatcaggt
    49861 ttctgctttg cccagtatgg gtgtggacgt ggcatgttcc cgcccattgg aactattgac
    49921 atggactcaa gtcctgcacc aatggctaca tcagcccatc ctgcctgaat tcgtgcagca
    49981 gaaattgcta ttgattccag accagatgaa cagaatctat taacggtagc accacaggta
    50041 tctattggga atcctgccat caatgcagca attcgaccaa cgtttaaacc ctgctcagct
    50101 tccgggaatg cacaacctac cataacgtct tctactacct tcttgtcaac ttttgctctt
    50161 tccattaagc cctgaagagc ggttactagc aggtcttcag gtcgggtctg agcaaaagca
    50221 cctttgcctc tacggcaccc aggtgttctg acagcctgta caatatatgc atctctcata
    50281 ataattttcc tcctgtttgc gaaagattat atgactaaat aaataatggt ttaccagttt
    50341 gtaagatatg cgctgccatc ttctgggtat tttcagtctg ccacagttca acaaaggctt
    50401 cacgctcctg tttcatccac tgttcttcgg taatttcagt accgcttgga acatcgccac
    50461 ctgacatgac aaacgcaatc ttcttggcaa tgaattccat atgaggtgta atgtacatac
    50521 ctatcctcat gttgagcatt tcagcttcta ccatacccat agcttcacgg ccaattacta
    50581 caaacttttc ttttttaggt ggagcatatc cttcatctat cattctgagc acttcttttt
    50641 tagcttcacc aatcaacagg tctctgttga atactacgcg atcttttgca gtcaggaagc
    50701 cttttttctg ggcttcaaaa gctgacatgg ttacctgagc ctgtgcaact gccatgaaac
    50761 atggcaggaa taatcctgca aggtcagtta tcttggcagc ttttggaaca gaattgagat
    50821 atttcttcca caggttgagc atacctaaac cgccaggaag taatccagca cctatttcaa
    50881 caagacccat gtacagttca gaatgtgcta caatacggtc agcattcaaa catacttcgc
    50941 aaccaccacc caatgtcatg ccataaggag ctgcaacaac aggtattgga gcatattttg
    51001 ctttcatcaa cccggcatgt acattagcaa taaacttgtc aatttcagac catttctttt
    51061 cttttgcaag gctacccata taagctaagt cgccaccagc tgaaaatgca cctggcatgc
    51121 caccagcctg gttgcctata acaataccag caccattttt caatacgaag tcggtagcgt
    51181 cagctataaa gtcaactatc tgcccattga ttgcgttcat cttggtgtgg aattcaatac
    51241 agaatacatc atcacccagg tcaataagtg atactgattc gttcttcttg atttcttttc
    51301 cagcaccttt gaggttgaac agagaaatag ctgcagcgct cttcttgatt tctttgtagc
    51361 cgccgtttac taagtcatag tagaattctt tgccattttc aactttgtag aatgaagttg
    51421 cgcctgatgc cagcatcttc ttaacgtttt caggaacctt cattccttct ttttccattc
    51481 tttcaacaga ctcttttacg ccaatagcgt cccatgcctg gaatggacct aattctaagt
    51541 tgtaacccca tgtcattgca ttgtcgattt caactattga atctgctatt tcaggaatac
    51601 ggttggctgc atagattgct cctaatgcag tgagcttcca tgcaaatttt gctgccttgt
    51661 cattgcccca tactatagct ttgatctttt gtgcggctgt tggaagcttc tttgcagctg
    51721 atacagattc aaactctggt tttgcagctt cgcgatattc taatttagca gggtcaagta
    51781 ctaatgaaag tttcttgcca tcagcagttt tttccttctt gtaaaaaccc tgtttggtct
    51841 tgtcgccaag ccatttgttt tcaatcatct tgttgaacca tgcaggaacc ttgtaaacat
    51901 cacgacgctc gtcatttttg agcagttcat aagagttttc tgcaaggtga tgaattgtgt
    51961 caagaccaac catatcagac agtttaaata cagcagtttt tggacggccc attgccggtc
    52021 ccataatagc atcaacttcg tcaatggtga tgccttcaga ttctaacagc tgcataactg
    52081 tcatgatttc gtgtacacct atacggttac caataaagtt tggagtatcc tttgcccaaa
    52141 caataccttt acctaatcgt ttttcaccaa attctgcaat aaaatcaaga acttccttct
    52201 tggtcttttc gccagggatg agctctaaaa gatgcatgta gcgaacaggg ttaaagaagt
    52261 gagtacccat gaaatgttct ttgaatgcat cgctgcaatt ctttgacatt tcttttaagg
    52321 gtaatcctga agtgtttgat gttacaacag cggttggttt gcgaatcttg tcaatcttct
    52381 ggaataattc ctgtttgatc ttaagatttt caacaacaac ttccacgatt aaatcacagt
    52441 cttttaactt gtcaaaatca tcttcaagat tacagagact gattcgcgat gcattgctct
    52501 tatccataat aagagctggt ttagctttca tcatgttgtc aaaaccagcc tgaacaatgc
    52561 ggtaccgtgc tttgggatcc ttcttctctt catctgttaa attgaatgga acgatgtcga
    52621 agaggagagt ttccacacct gcgtcagcca atagagccgc gataccacca cccatgatac
    52681 cggagcctat gacggctgcc ttttcgattc ttctaaccat aagaacctcc tactgagatg
    52741 taataattaa ataaaaattt gcatactcct gcataaatac acaacttaat acaatgatga
    52801 aatatcagat taaattgtca atatttatta ttaaaaatca gggattaatt taatattttc
    52861 agtatataaa atttaattta tttttattaa aatgaacata aattgtaata tgttaatact
    52921 gcatttacca ccagttgttc a
//
LOCUS       DAORYL010000012        51225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_554_length_51225_cov_12.600879, whole genome shotgun sequence.
ACCESSION   DAORYL010000012 DAORYL010000000
VERSION     DAORYL010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 51225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51225
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_554_length_51225_cov_12.600879"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..475)
                     /locus_tag="PLV81_04055"
     CDS             complement(2..475)
                     /locus_tag="PLV81_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09311.1"
                     /translation="MKTVKQYVIVVLAAAVSCLLSCTSVPKYESPVEPIIAPVEVNVN
                     FRFIAHDISIANPDDDKRCYYKVFIDKIDAGRTTIGLESQKKYFEAKLSPNTHLVVIE
                     KWVLDEREGEYKKVNNILQPKPNYYYFETKSNAVTEVIMVNDKKTNTAQYSVSIK"
     gene            complement(472..2031)
                     /locus_tag="PLV81_04060"
     CDS             complement(472..2031)
                     /locus_tag="PLV81_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09312.1"
                     /translation="MAKIQLSLSMVLCCIMLLSSCKKETLLEIEPNDTYTQANLISVG
                     KPVNGFINTESDFDFYMVDVNEPVLFDISVSAIKGVNISFTVWKAGDDGIVPLKYVDD
                     MRKSAPERLRGFKVEPGRYYVSVSHGDKDQRIKNTENAYTLLINQEDAEGFESEPNDT
                     ILSANPIPVGVPVRGYYSPAFNKANTNAVNPNREEDWFMFYVEEVPVVCDVEVTGVPG
                     IESQLDIVDAQGTVLFSSNPQGQGVSQIAKGIGLSVAGNYYIMVAARNYSANNETPYS
                     VTVTIRGYDNTTEIEPNNILQSATPIVTDTIMGRIYPDSDVDFYSYSGDSSENKWYRV
                     EVTPPMTLDILFAIYNTKGEELYSVNNESTGQKELHPNLFIGTPFYVKVWAKRGQLDL
                     DNYYTVAIVPVNISDMIDIEPNDTKEQANTVQGNSITGYVSKKADADYFILQYKGRQK
                     KYFVVTAPEKIPVSFSVTDSLGYILQTAKVKAGKSATVHEIIDGKGYCIVKPLNDTYD
                     GIYHIEIRDKP"
     gene            2224..2538
                     /locus_tag="PLV81_04065"
     CDS             2224..2538
                     /locus_tag="PLV81_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09313.1"
                     /translation="MTTKSKLYVAGIVLAILLLSGVYLYFKYFFTYEQKNIVQRKIET
                     ITGQNLTITVFGYDGRIIKRWYGVQKITTPKDGRNYSYFYTREGEYVQIPASVWYIAE
                     EE"
     gene            2541..4625
                     /gene="recG"
                     /locus_tag="PLV81_04070"
     CDS             2541..4625
                     /gene="recG"
                     /locus_tag="PLV81_04070"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOM09314.1"
                     /translation="MLDQSIQYIKGIGPKRAELLKQELGIETVEDLLYFLPRKYIDRS
                     NTKLIKDCFVNDIVTVTGIVKQVKISGHRRKFLEVVIDDGSDTLSGVFFGGIQYFLKL
                     FEVGDTVLFSGKVDYYTTKQIVHPEYDFIGDSSDVLHTGRIVPIYPSTETLKSHGFNS
                     RVFRKIIYNALELAKQHIQEPFDSEMVERLNLMPLSQAIQHIHFPATMDEAHHARVRL
                     AFNELFFLQYYLHLSRHYIRETHKHAKPPINDSLYSDFIQTLPFKLTDDQIKAVQEII
                     NDLQADFPMNRLLQGDVGSGKTIVSMIASLAVISRGQQVAFMAPTEILAQQHYATFKK
                     FLPDSVTVQLLTGSTPAAVRKEILAGLNNGSINIIIGTHALIQEHVQFANLGFIIIDE
                     QHRFGVNQRASLRTKGYMPDLLVMTATPIPRSLSLTLYGDLDISIIKQKPANRIPVTT
                     LAFPSSKLEAVYRSLEKYMNEGRQIYYVLPLIEDSEKLDLKSATATYTQLVKRFPTKT
                     IALLHGKLPNEEKEKIMQQFNNHTIDLLVSTTVIEVGIDVANASIIVIEHAERFGLSQ
                     LHQLRGRVGRGEYKSFCVLIYPDDIPQESMQRIHILTTTDDGFVIAEEDLKMRGAGEF
                     IGTRQHGQGIAFEFADPVNDFELISIAREEAQKQVERLKDIPALFENIKRTQAMNILE
                     GLRKKHILALLS"
     gene            complement(4631..5332)
                     /locus_tag="PLV81_04075"
     CDS             complement(4631..5332)
                     /locus_tag="PLV81_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOM09315.1"
                     /translation="MFTVKQYKGDENLKKILDKNNIPLDIYNLKAAILGVQASAGSFT
                     LRSLIKDFFSEEVLENKDVADQIESLWFYLLRLQRFDDSELEQPDFDMKTREAYTAYV
                     NASIMRAETFLKYLSTGQLDEMLKNEKVNKIYSIFIGNVNLLKALIISMEEKWSQEDF
                     ASDTTFLKKLTGFVRVMWDTQIALKEHAKIFNLQKMNNKNIIEAIQQQTGTEIGRNDP
                     CPCGSGKKYKNCCGR"
     gene            complement(5347..7008)
                     /locus_tag="PLV81_04080"
     CDS             complement(5347..7008)
                     /locus_tag="PLV81_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09316.1"
                     /translation="MIDDELDRLLQDIDSDKDYTAQKAHAKHFKEHVLDSELDETIHD
                     IPLPEKKNISEVVKFFKRGLSSGIHRHIELAKLLRSRLDITEVPEQIVPVIKKSSYSI
                     FKTIEDIEILTDKFIGEINEANLRRKATKDFPLYFPDDYPDDNRLDFIVLKEIYLLLR
                     SFNSIVKKDWTELEKQSAVFNFAEGGKQLSSVLNDIIVEGNSLCKATDVLLELIANHL
                     GLLSHHYNLSDADIRSKLQFNEFKSYTVQSLLNELDTSEFVQDQSEALKHNIHKTSTL
                     TSPDGMEEASDSNEYNKQKQYEISKSKTQSEYSTQQSTVPFTVKGTKPWNTREPYILS
                     VDENVLKIEQEDLEYMVYYTKTQLTDDQMKSELRRALLKLSIDKEKYDIVEDFIEFIS
                     KKNLSILHESVEYFDIPENDQWLFYYHLGPHTIFRLLLADIQISGEGYCFSSRDGKKI
                     KKLIPYEFLKTIVLEWCDNNVNNRDLPFDSVNALDLIRRQVSKKYFSEIDEFVAKIDA
                     YLGTLNPETLKKLDRKSFIKQKALQVYNQKQILIFNRFVDRTIFK"
     gene            complement(7025..8947)
                     /gene="ftsH"
                     /locus_tag="PLV81_04085"
     CDS             complement(7025..8947)
                     /gene="ftsH"
                     /locus_tag="PLV81_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOM09317.1"
                     /translation="MNKAAKQVALLLLIALLAIAIFRMNDMNTQKVEQLQYSDFIKKV
                     YENKVKSVTIVNGKKIEGTFTKGDKVYLFETVIPYQDPDLIKTLINNRVEIKGEEVDD
                     NLFWKGVLNFLPWLLFFGFIWFFMIRQIQSTGNKAMSFGKVRAKLQPETKNKVTFADV
                     AGVDEAKVELQEIIEFLKDPKKFITIGAKIPKGVLLVGPPGTGKTLLARAVAGEAGVP
                     FFSISGSDFVEMFVGVGASRVRDLFDQGKRNAPCIIFIDEIDAVGRLRGAGLGGGHDE
                     REQTLNQLLVEMDGFETNEGVIIVAATNRPDVLDPALLRPGRFDRQVVVDIPDIKGRE
                     KILAVHTRKIPLAKDVDLKVIARGTPGFTGADLANLVNEAALLAARRNKKRVSMLEME
                     DAKDKVLMGPERKSVLISAEEKKVIAYHEAGHALLGILTGADPVHKVTVIPRGRALGL
                     TMHLPKEERHLHNREYWLNQITILFGGHVAEKIKFGQVTTGSSNDIERATDIARRMVC
                     EWGMSETLGPLAFGKKTEQIFLAREIAQHRDYSEETAQLIDKEVHDIVTSCRDRAIKL
                     IEENSDKFELLATNLIERETLNAQEIELLMKGEPLPPIHNGENPQQPDEEPKIEKIKK
                     KASQKPLLDDKEVIPT"
     gene            complement(9119..10522)
                     /gene="tilS"
                     /locus_tag="PLV81_04090"
     CDS             complement(9119..10522)
                     /gene="tilS"
                     /locus_tag="PLV81_04090"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOM09318.1"
                     /translation="MNNLVQKVYTFIQDNNLIVPGDRVLVSVSAGKDSMALLHVLYML
                     REHLGCTIAMYHLDHKVRGEESFGDLLFVMNQASRYGIPAFIERFDFAKHQEPGKSFE
                     QQAREYRYTRLHMIAQHYGYSKIATAHTKNDQIETLLMRMFQGTGLQGLEAIALAQGI
                     IIRPLLVLSQDEVYAYLKHNDLSYRHDCTNDDTTYLRNYIRHSVLPVIQKRFPHFDEA
                     LLNLQKQAHDATRSINDLMHTLYPGFIQEQPGLIQVQENAIAHSEYIFKFYCAYIISN
                     YLKKYVDSDILDALYRAHFSKKKNITLFEGKGIYIYRRYRTTTSFLIFSSQPLCMGDS
                     NYEYNLPVGKTVTVTQAGITVQCAVAESIDVQSSESGTLILRYTGCDHIVIRNKRTGD
                     AIVRKTGKRTLKRLYIDYRLTPEQKNLVPLIVIDNQIAAVLFDVTGAIKAEVSPSFLP
                     ENGQKMLVIRYWHSNNV"
     gene            complement(10610..11083)
                     /locus_tag="PLV81_04095"
     CDS             complement(10610..11083)
                     /locus_tag="PLV81_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09319.1"
                     /translation="MYIETTTCISYKHLEILKFYAEKKNMSLRTFVSSIISFAALCEK
                     GDIQYFKQIQYRTRNEGEWKRLHLVLYNDEYEFFLDVKKLWKMSLAKVIAYCLDNVLY
                     EFLEFLAKAEEDEDYYTDNYRYSGYTFEIGDEENIFFCKFYWGPHPTIVQKALSA"
     gene            11380..13122
                     /locus_tag="PLV81_04100"
     CDS             11380..13122
                     /locus_tag="PLV81_04100"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012927529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOM09320.1"
                     /translation="MDITIQKRIEQWQNPPFDTSTIQEIKQLVAQNNEKELYERFYTT
                     LEFGTGGLRGIIGAGTNRMNIYTVGMATQGLANYIVHQGNAAKGVVIAYDSRRMSDVF
                     SKEAASILAGNGIKVYLFKDIMPTPICSFAIRHYNATAGIVITASHNPPEYNGYKVYW
                     EDGGQVVPPQDKEIIREVSKIQDITQIKKMNFDEAIAHGIILLVGDEIIESYAQKLSQ
                     VRFKSDELSPIHIVYTPLHGTGYRIVPQILKTSGFPNVHIVEQQAIPDGNFPTVKSPN
                     PEEPEALTQAIELAQQINADIVLATDPDADRMGVAFKDDNGQYMLINGNQIGSMLLYY
                     ILLRLQQLNKLPKNGFVVKTIVTTDLQQEIARHFNVTIENVLTGFKWIAKKMKDYEKS
                     HVFIFGGEESYGYLPLDFVRDKDAVSSCYFFAEMAHWLHSHNKTLKDFLYEIYTQFGY
                     YNEKLVSLTLKGVEGIQKIESIMKHFRSNPPKQLAGTTVTAILDVKQGTLTDVMSGTT
                     KAVDLPASDVLQFTLENGSKVTMRPSGTEPKIKFYFSSRKKGTDIKQLEEEVSRYNET
                     LAKELLSTIESVIT"
     gene            complement(13332..13775)
                     /gene="ybeY"
                     /locus_tag="PLV81_04105"
     CDS             complement(13332..13775)
                     /gene="ybeY"
                     /locus_tag="PLV81_04105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOM09321.1"
                     /translation="MAIKVNVEREHITMPKELAAFCKKIIAAVLQGQEVDNVVVTLIL
                     TNDEAIAHINKTYRKKKGPTDVISFAYREAPMPHVTTKEHLGDIFISLETAQRQAIEY
                     GVTLKEELKRLLVHGVLHLLGYDHEQSLYKKRKMQKKEEEIMQKI"
     gene            complement(13759..16011)
                     /locus_tag="PLV81_04110"
     CDS             complement(13759..16011)
                     /locus_tag="PLV81_04110"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOM09322.1"
                     /translation="MLNSFMVFKRGIQFAFKKKSLKASFIYTLIVIITATALLTLNRI
                     GATYSYQAGDIAREDIRAPMNIEYPVAIETEKEKNRIAEAMPAIFDLNESIVEDKLKI
                     SNMLCEAVIKVKEQMPDSSPSKQVLLLRTMLPDYLQYSDRVLMGLLQYDEQLLHRDLN
                     KILLQIYNNGILEKPYTNPLRLQSNHVIVRVVGSASELKEKQLNVDALVDMKKIQSLV
                     PVISKDILNKPSSDALYAVNSVIISLLQPNLFYNEEETKRRIAEAVQSVKPVTRVIKK
                     GQSIAREGDTITEDMLQKINIINKNAHFSGSSFIIGLLLIQLGFLVIFGYFLITYYSN
                     LVVDNKMPLIVFTLVLAFIVIAFFIARWQGRAFEGLFFPLLLPIPLVTMVLAIMYNMH
                     IAMLIGVYIVFFVTVSMKGDFTTLLLAFSSALSGVFVVGDVERRSDFLRGGVTLGLIN
                     SVIVIAVGLMQEMQFGIIANNVGLAFISGIINAIIVFGVFPVYENLFGVTTKFKLLEL
                     SDLNAPIFKEMLIKAPGTYNHSLMVATLAESACKEVGANALLARVGGYYHDIGKIQDA
                     NFYIENKVTDPRAKDMSALEYCRLIISHVEKGVELARKNNLPEMVIDFIREHHGQTVM
                     TYFYHQALEEGTTNGNSIQKSDFQYPGPKPHTRETAIVMLADAIEAASRSIQEPTLEK
                     LETLVKRIIYNRLNEGDLERADITLNELNKIQNAFVQVLQGIFHSRIEYPTKEAINRL
                     EKKSSNGNQG"
     gene            complement(16030..16989)
                     /locus_tag="PLV81_04115"
     CDS             complement(16030..16989)
                     /locus_tag="PLV81_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOM09323.1"
                     /translation="MVDTKFEIDDISIYKRICGYKDKNIKAIEKLLDVKIIPRGNTLI
                     VEASGDNAAKALQLLHLMGDYLYMRDSLYEFDDFDIRYLALSINQGIKVDADEINKLK
                     IVIPETGKTIVPKTINQANYMSAIHKSPITISIGPAGTGKTYLAVAVGVKFLLTGRVE
                     RIILTRPAVEAGESLGYLPGDFIQKINPYLRPLYDALFDILPYERISKYMEKGIIEIA
                     PLAYMRGRTLNKAFIILDEAQNTTSSQMKMFLTRLGNSSKMVISGDITQIDLEKPRQS
                     GLLAAQKILHGIEEITFIEFSKEDICRHPIVEKIIAAYEKHAE"
     gene            complement(16989..18764)
                     /gene="aspS"
                     /locus_tag="PLV81_04120"
     CDS             complement(16989..18764)
                     /gene="aspS"
                     /locus_tag="PLV81_04120"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOM09324.1"
                     /translation="MFEQRNYCGELTVNDVGTMVRLAGWVDTKRDLGGIIFIEMRDVT
                     GIVQVVFDASRNSTAYENADKVKNEYCIFVTGQVAKRSDETVNPNLKTGMIEVIGQDI
                     SILSESEVTPFPIDTYTNLNEEVRLKYRYLDLRRADMQEAIIVRHKVMQTIRRYLTDN
                     RFIEVETPILNKSTPEGARDFLVPSRINRGMFYALPQSPQLFKQILMISGFDRYFQIA
                     KCFRDEDLRNDRQPEFTQVDLEMSFVNPQMVMQIIEGLLKEIVREVLDKEIETPFPVF
                     TYDEVMQRYGKDAPDTRFGLELNDVSDIFTNSTFNVFKAALENGGIIKAIVINDEGKI
                     SRAMIDEYINYVRTFKAMGLPSVRYTGSAFETGIAKFLSDSEKQKLVDRLKLNSNALI
                     IFSCDKPKIVNDALGNLRIKIAQQLNLIDENKLNFLWVVDFPLLEYDAEEGRYYAMHH
                     PFTAPKPECEPMLDTVTPQTTQDIKAQAYDVVLNGVEIGGGSIRINRMPLQKKMFGIL
                     GISEEEAKIKFSFLLDALKYGAPPHGGIALGLDRILMLLLKRNSIRDVIAFPKTQKGQ
                     CLMSEAPSPVSAQQLKELSIKVVEL"
     gene            complement(18950..20305)
                     /gene="glmM"
                     /locus_tag="PLV81_04125"
     CDS             complement(18950..20305)
                     /gene="glmM"
                     /locus_tag="PLV81_04125"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOM09325.1"
                     /translation="MSLMKSISGIRGVVGESFTPDLITRVAMAFARHIHGGNVIVGRD
                     SRITGQQIAQCFESVLSLCGCQVIDIGVVPTPTVQIMVEHTKAKGGVVISASHNPIQW
                     NAFKLINATGSFFGPDEMNTFLSLVDTITPFGAKWNAIKPVMCDSSAANVHIEKVLAA
                     IDVAAIKKRKFTVVLDSVNGAGSLITQELLKKLGCRVIPIHCDMTTGTFPRGAEPVAQ
                     NLKALSKAVKEHKADIGFAQDPDADRLAIVNEKGNPIGEELTVTLAALRCLAMKKGPV
                     VVNMSTTKAIEDVALQFGAPVYRSKVGEIHVVEAMKQYRAVIGGEGNGGVISPEVHYG
                     RDSLVGIGYCLELMAHRKEPISQIVASLPAYYMHKDVMNISGEFDTGTIAQKIKEQYT
                     DEVINILDGIRIDFVQHEAFQGGWVHLRPSNTEPIFRIIAEGKSKKQCEAIVNTFKTM
                     K"
     gene            complement(20539..23115)
                     /gene="secA"
                     /locus_tag="PLV81_04130"
                     /pseudo
     CDS             complement(20539..23115)
                     /gene="secA"
                     /locus_tag="PLV81_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004758870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
     gene            complement(23279..24385)
                     /gene="proB"
                     /locus_tag="PLV81_04135"
     CDS             complement(23279..24385)
                     /gene="proB"
                     /locus_tag="PLV81_04135"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOM09326.1"
                     /translation="MPRSAYLKHVKTIVVKVGSSLITDESGISKKQVTRLVDDVVFLL
                     KKKYRIVIVSSGAISAGSAVMKKKRNLCTIPEKQALAAIGQSILMDVYRECFNKYGYE
                     VGQILLTEDDVKHRRRFLNARHTLNALLEMGVVPIVNENDSVVIKEIKFGDNDTLSAH
                     VANIVQANLLVLLSDVDGFYWDEGDTEPISEIHEINDEVRKRCRGAGSEYGTGGMYTK
                     IKAGEMVIQSGEMMIIANGRTEHILQRIMQGEKIGTLFLGKTQLKSKKRWIAFNVKPK
                     GKLIIDDGAVKALKENKKSLLAIGITQIEGNFDLGDAVEITDKRGTLIAKGIVNYTSG
                     ELEKIKGKNTQEIKKIFGSKYYEEVINRDDLIVF"
     gene            complement(24390..25373)
                     /gene="obgE"
                     /locus_tag="PLV81_04140"
     CDS             complement(24390..25373)
                     /gene="obgE"
                     /locus_tag="PLV81_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOM09327.1"
                     /translation="MAKFKDNIVIKVRAGKGGAGAVSFRREKYIPKGGPDGGDGGKGG
                     DVIIQATQGYYNLGHLFKDRIYKAQNGGFGMGQNRHGKDGQDLIINVPAGTQVLDAES
                     GELLADLVREGNTVCVARGGMGGKGNAFFKTATHQAPRFAQPGMPGEERSILLNLKLI
                     ADVGLVGLPNVGKSTLLKAITNAQPKIADYPFTTLIPNLGVIERGETVIKIADIPGII
                     EGAHKGLGLGLSFLQHIERVRIILFCIEAIDADPLYTLSLLKAELSTYNKDLPQRTSM
                     ILLTKSDSVNESVILKRVALLEKEKYTVLPISSLTGYNMDILIEKLFLLME"
     gene            complement(25802..26062)
                     /gene="rpmA"
                     /locus_tag="PLV81_04145"
     CDS             complement(25802..26062)
                     /gene="rpmA"
                     /locus_tag="PLV81_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015123382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOM09328.1"
                     /translation="MAHKKGGGSSRNGRDSRSQRLGVKVFGGQFVKSGSIIVRQRGTK
                     LHPGTNVKRGSDDTLFAVTDGIVKFERLDKERKKVSVYPVQA"
     gene            complement(26106..26459)
                     /locus_tag="PLV81_04150"
     CDS             complement(26106..26459)
                     /locus_tag="PLV81_04150"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HOM09329.1"
                     /translation="MITIIVQGIDVTNNHFSLSGSVFAVRITGHAGQASKGNDIVCAG
                     VSALSQSVALALEYYEIAHSISQQEGLLEFSVNLDSLLDDKKILCESLVSVFILGINE
                     IRKQYPEFVRVSIKE"
     gene            complement(26459..26773)
                     /gene="rplU"
                     /locus_tag="PLV81_04155"
     CDS             complement(26459..26773)
                     /gene="rplU"
                     /locus_tag="PLV81_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOM09330.1"
                     /translation="MYAIVEIAGKQYKVEKDKTIAVDLLEAKDEETIAIDKVMMLVDG
                     DRVLVGQPYLSNVEVKAKVGGVVRGKKVRGIKFKKRKNYTRTLGHKQPYTSITIVDMS
                     VK"
     gene            complement(26838..26911)
                     /locus_tag="PLV81_04160"
     tRNA            complement(26838..26911)
                     /locus_tag="PLV81_04160"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26875..26877),aa:Ile,seq:cat)
     gene            complement(27111..27653)
                     /locus_tag="PLV81_04165"
     CDS             complement(27111..27653)
                     /locus_tag="PLV81_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09331.1"
                     /translation="MVYKKGKGIIHCFKYIAALLVITLSVNFIGYPLMAQTIESDAGK
                     KYKDVPYQDEFSKKAQERREESPTMQRDPRLACLLSLIVPGGGHVYLRNDLKAAGFCL
                     LTATAYSVSAYYLYVALIKTDSSTEKKSRLILSGLLFVVGALIHVVGIVEAYNDAIEI
                     NEKQFYFGTEKSSSPYVARN"
     gene            28002..29810
                     /locus_tag="PLV81_04170"
     CDS             28002..29810
                     /locus_tag="PLV81_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013798578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOM09332.1"
                     /translation="MIKEIINKSKSELIELLWQINPEIYDLLKNSRNLDEAREFIMDY
                     FDVLDREFFNIKSKSDYHKMHIIIKNQAKECIRIAKNIMRTENEKICNFSALEVLFKI
                     AKGDTSELEKISGGFIVELIYLFKGIRGQSDVIHDNEVIPSDDHEFSKTRSERLDYYA
                     ENMYKRLSQYKTGFDDEIIEQRKKMKQKILKYFGSDEQDWKNYKWHLKNIIRSKNILK
                     DIIKLEEDEIEGLDYAEKYNIPFEITPYYLSLFNENGRSRFDRAIRAQVLPSKTYCEN
                     VYNNKKNNIDMDFMGEKSTSPIEGITRRYPFIVILKPVNFCPQICVYCQRNWEIEPST
                     NLHINKTTLENAINWITNNKNIIEVLVTGGDPLIMTDKYIENILLELSQIKHVERIRI
                     GTRTPVTLPFRIKDDFVKMIKKYHKPGYREICIMTHFEHVAEITPEVIEAVQLIREAG
                     ISVYNQQVFTYYNSFKYETSALRRMLKLIGIDPYYTFNTKGKKETIDYRAPIARIEQE
                     RKEEARFLPGVIRTDEPVFNVPKLGKSNLRAWQDHDVLMIKSRGERVYRFLPWESNVS
                     LVDDYIYTDIPIYNYLKRLHNDGENVEEYETIFYYY"
     gene            complement(29828..30868)
                     /locus_tag="PLV81_04175"
     CDS             complement(29828..30868)
                     /locus_tag="PLV81_04175"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOM09333.1"
                     /translation="MNELNLKDKKIVIFGTGAIGATLAAWLYPFHDNLYCFDKKEITD
                     VIDINGITVYHIDSPDKRENYKIKTMHKIEEISDADIIIFCVKNYSLEGCAKAVKDVL
                     KNSATIISMANGIDNQYILPKYFDKIIYGVISYNAWIDTPGVAGYQKKGPFILGTPDN
                     SLMDEMQVIADYFSNGVETIVTKHLQDAVHSKIVINLTNALTTLIGHGYREISDIDTF
                     QRLLSNTLYEGVKIVKKAGYNECKLGGMPPWILIKLSAKLPRLITRPLFLKNVKKMVK
                     SSMSQDIIQRGMHMSELDSLTGYIVKLAKQNGIKAPYNGTIYNLCQERFNQSKFEPMD
                     VKEVWERVKENL"
     gene            complement(30884..31777)
                     /locus_tag="PLV81_04180"
     CDS             complement(30884..31777)
                     /locus_tag="PLV81_04180"
                     /inference="COORDINATES: protein motif:HMM:NF018066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HOM09334.1"
                     /translation="MKPSFDIPQTMLNNEDKFKDPFFKRFTLRHADWPIKLTDKIQKN
                     YKFPTFYGDVTCAMAICFCSYNRAKDIVQETLGKKVYPVSMLKGRAIVAFSCYEYKNV
                     MNINPYNEIAIAIPILVNQKIKVPVLPMVLKTFKHFGYFIAGMPVTSYENTLRGHKIW
                     SLPKVTHEIEMIHTDEMCTTIAKDSDGKEYLTVKVPKKGKKQEFDETGYLYSRHNGKI
                     IRSKTCFKATFNVNTYFSHLFKKDVEGNFIEIGNSKEAEYLNYLDIEPHPFQFRYAEH
                     MNSCFDLPDENPPSWLKKLND"
     gene            complement(31896..32492)
                     /locus_tag="PLV81_04185"
     CDS             complement(31896..32492)
                     /locus_tag="PLV81_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOM09335.1"
                     /translation="MSKFPKKRSITSRTDIQEKAIKLFQKYGTLKTSVNDIVKECNIA
                     KGTFYNYFQSKDDLINSIFEEYLNEFINNVISKIQYRAEINYFTESIIDFFDKNKLFL
                     SELRINMVSNKKLKYTENTIMFFSNFIIKYINKKYDYRITQYDEYAKIIFFSILELVH
                     KYSIEKQIKSKDEATNMLKDLLKRFFDCKQDILIPRSE"
     gene            complement(33112..34185)
                     /gene="dgcA"
                     /locus_tag="PLV81_04190"
     CDS             complement(33112..34185)
                     /gene="dgcA"
                     /locus_tag="PLV81_04190"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcA"
                     /protein_id="HOM09336.1"
                     /translation="MNDDMSLYDEDIKFPIEIEQYERKIYDLKQLIEISKGLNSTLDY
                     NVLIDSILLTCMGQMQLLKAGIFTHRSIDRQEFVLHRNYKGFEIDHKNEYLIPTNSKL
                     IQFLDANFRCYTLNELKELLPDEPSLENLIRLDPSLIVPLKGKGTLNGIIVLADRINQ
                     KGFSESDKEYMLNIASLAGIAIQNAYLYELATTDMMTKLKIHHYFQTALIEEREKSLK
                     SSLPLSLIMMDIDHFKNFNDTYGHSCGDMVLKNVASVISSSVRQSDIAARYGGEEFSV
                     ILPHTDINEAFKVAERVRISVQKSYVLFKRKKLGVTVSLGITQFNPAIDISTKNIIDR
                     ADKALYVSKQQGRNRTTVLLSDK"
     gene            complement(34198..34917)
                     /gene="hisA"
                     /locus_tag="PLV81_04195"
     CDS             complement(34198..34917)
                     /gene="hisA"
                     /locus_tag="PLV81_04195"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOM09337.1"
                     /translation="MLVIPAIDLKEGRCVRLVQGDPLQETVYSYDPESIAKQFEEAGA
                     KLIHVVDLDGAFEGQPVNMHIVKRIAKAVSIPIEIGGGIRTETAIKMYLDEGIERIIM
                     GTVLMEDTFREIAERFKEYIIAGVDAKDLKIATHGWKKIIDMDAIKCIKELQSIGISE
                     IIYTDIATDGMLQGPNYTSIEKILSEIPGIALIASGGISAIEDLEKLKKYENHGLKGC
                     IVGKAIYDGRIDLRNAITLCD"
     gene            complement(34908..35516)
                     /gene="hisH"
                     /locus_tag="PLV81_04200"
     CDS             complement(34908..35516)
                     /gene="hisH"
                     /locus_tag="PLV81_04200"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOM09338.1"
                     /translation="MITVIDYGMGNLRSVVKAVEKYTSDVQISSDPGSIATSKALIMP
                     GDGAFGHAMENLTTLGWVEPLVDFIRGGGYFFGICLGYQLLFSTSEEFGHHKGLDIIP
                     GNVVRFTNDLKVPHMGWNQVVFKQHHPVVDGIPQYSYFYFIHTYYPVVLDSSWIIGQT
                     QYSVEFASIVGKGNCIATQFHPEKSHKYGLRMIENFVRLACS"
     gene            complement(35513..36097)
                     /gene="hisB"
                     /locus_tag="PLV81_04205"
     CDS             complement(35513..36097)
                     /gene="hisB"
                     /locus_tag="PLV81_04205"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004697683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOM09339.1"
                     /translation="MERSAHIERKTKETDITVKLLLDSIEPGTIQTGIGFFNHMLTLF
                     AAHGRMSIQLNAKGDLDVDAHHTIEDTGICLGKAFQKALGDKQGIVRFGQAIVPMDEA
                     CSSVIVDISGRGYFVYRGDKLTGLIQTYSEELTLEFFKAFAHNAQITMHIQQLYGDNR
                     HHIHESIFKAAGIALYRAYTISDSTGIPSTKGVI"
     gene            complement(36116..38980)
                     /locus_tag="PLV81_04210"
     CDS             complement(36116..38980)
                     /locus_tag="PLV81_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOM09340.1"
                     /translation="MSKKSIHIIVTILVCVAAPLLIYPFGKNKVTIETFKWHILKTIH
                     CNVYYPYGMEQLATKTAVIAEKAYVHIADALQHELNDPVPIIVFPSHIDFQENNILLQ
                     IIGEGTGGFTEAFKNRVVVPFNGSYDEFQHVLVHELVHAFQFNILMRDSSGKMQSMMS
                     MGSVPLWFTEGMSEYLSIGFDETADMTMRDFFLNDQYISLMDFTTYRTGNMYYYYKVG
                     QAFFYYFEQVYGKGKIGEFLRDIRDLDNFEEALKVHTKKNLEEIDTEFKHFYKKRFYP
                     VIAGRNFDEEEGQRITNHQKTRSIFNTCPAISPDGKKIAYIDNRDIYSSISIITIGEK
                     EAEEKARVILKGNETSEFEGMHLLDNYLSWTPDGKGIVFVSQSKNRDVIFIIDASNGK
                     ILNEIVLPFRSIKDPALSPDGKFICFIGQTNIQSDVYIYSLHDKSLKQVTQDVYSERY
                     PKLAANNTVIFYSSNYPAGDYTSQQYAIIKHTLSTNAREVIVQPQSKNLQVDIAKDGN
                     SIVYISNREGIYNVYQYDISKYSETKITNASTGIFYPRIFPDANKIAFVSYQNGGYDI
                     FIKEDIKPVTIAPPFNTEHIPVMLPQSYFPLSQAIWDNYTTTVSPDWVFFGLGGTVGY
                     GFAGFAQMSFSDYLGDNRIVVTTDYLRYGSSANYLNWDVQYLYLRYRWDYSLGFFRQK
                     NPFGIFTLASINDLIHNAYWSTLSMDHYGVYAIASYPFTKFSRFSTRVSSSRYERDYS
                     YLDQRPDVFANLNSVSVSYNYDNVLWGFLAPLRGTRGQIMFTQVVNLTGQDYSFSSID
                     IDVRHYLFLDKQYVLAFRGIGGKIMGEKKEYFKYYIGGFNTLRGHPFIEYGGTNVFLF
                     NAEFRFTFIESIRMGWPLFLKIGNIGGVLFVDAGSAWDNTYTFMNKDTGEFQDFKMDM
                     GFGFRLTLYPIVILKLDYAWPYYYSGFGHKEIVFSLGYEF"
     gene            39090..39273
                     /gene="ssrS"
                     /locus_tag="PLV81_04215"
     ncRNA           39090..39273
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLV81_04215"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            39292..40335
                     /locus_tag="PLV81_04220"
     CDS             39292..40335
                     /locus_tag="PLV81_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09341.1"
                     /translation="MEIITVFGVRWRVAPLWEVGLTVLVILLVIAFFALLKYNQYLKL
                     KKSSLEQIFIYKARQRGLTGYQVRILQHIVALSKLSSPEIIFINKALFETGLVKFLQY
                     MAKLKEPPDDLKDIFKDIVITYERIYNPVEYQQPLDSIEDISADTLVCGYSQTGRAFI
                     AKIMLIAEDTIHCRYFRKPGQLPDEIITEPVSIFFFRNGDAEYSFESEITAIQENTIL
                     LKKPDAIKRGKEVHHPYIQTLIPCTIQKKKKVDKEEEEAIIQHDDNEPVNGTIERLNE
                     FEAVVRVEKTLQYQNEYTLFFTLENFKIEIVSKILASRTITKEKIIYYTFKLIEMSEA
                     AQNVISKYVKERL"
     gene            complement(40355..41467)
                     /gene="dnaJ"
                     /locus_tag="PLV81_04225"
     CDS             complement(40355..41467)
                     /gene="dnaJ"
                     /locus_tag="PLV81_04225"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOM09342.1"
                     /translation="MATTKRDYYEVLGVPRNASEDEIKQAYRKLALKYHPDRNKGSKE
                     AEEKFKEATEAYEVLRDPKRRAQYDKFGHEGVAGFEGFGRGAYTDFSDIFGDFDLGDI
                     FESFFGAGFGTRTRSTRVRRGRDIQYDLSITLEDAALGKEVQIEIPRHEVCSACGGTG
                     SASGTKPTVCPVCNGTGQIRQTQGFFSITQTCYKCRGEGKIITSPCRVCKGTGLEEKH
                     RKITVKIPAGVESGSRLKIAGEGEQGPGGGPAGDLYVVVHIQKHPMFERHGNDLISIV
                     DVSFAVACLGGEIEVPTIYGKKAKMKIPAGTENGQIFRLKGNGMPYLGSYGKGDQLVK
                     INIKVPKKLSPRQKELLKEFARIEGETVDSDREFYT"
     gene            complement(41547..43469)
                     /gene="dnaK"
                     /locus_tag="PLV81_04230"
     CDS             complement(41547..43469)
                     /gene="dnaK"
                     /locus_tag="PLV81_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017852604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOM09343.1"
                     /translation="MTMSKIIGIDLGTTNSCVAVMVGGEPVVIPNSEGQRTTPSVVAF
                     TDKGERLVGQVAKNQMITNPENTIRSIKRFMGRSYSEVSSEIKMVPYTVMDDGKNGVK
                     VKTIAGDFTPQEISARILQKMKQSAEDYLGEKVTRAVITVPAYFNDAQRQATKDAGRI
                     AGLTVERIINEPTAAALAYGLDKKERNHKIAVYDLGGGTFDISILELGDGVFEVKSTN
                     GNTHLGGDDFDQRIMEWLIAEFKKQTGIDLSTDKMALQRLKEAAEKAKIELSNKLQTE
                     INIPFITADQSGPKHLIMTLTRSKFEGLVEDLIESTKEPCIKALEDAGLTPGDIDEVI
                     LVGGSTRIPAVQELVRRVFGKEPHKGVNPDEVVAVGAAIQGGVLAGEVHDVLLLDVIP
                     LSLGIETLGGVMTKLIERNTTIPTRKSQIFSTAADNQTAVSIHVLQGERELASQNRTI
                     GRFDLIGIPPAPRGVPQIEVTFDIDANGILHVSAKDLGTGKEQKIRIEASSGLSEEEI
                     KKMVKDAELHAEEDRKARQLVEARNEADALIYSLEKLIRENEGKIEASEKASIEAEIE
                     NLRKAMESTDINVIRDAKAKLERASHSFAERLYKQGAEQYAKSQQPETGEQESAQSGE
                     KVYDADYEVVDDDKKK"
     gene            complement(43525..44268)
                     /gene="grpE"
                     /locus_tag="PLV81_04235"
     CDS             complement(43525..44268)
                     /gene="grpE"
                     /locus_tag="PLV81_04235"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOM09344.1"
                     /translation="MSGKDKKHHNNEQQHNAMSKEIHDHNKELHEHMNAQEPVGDSST
                     NQPSGKEEPQAAQDQLQQKEDALQKLQEELNKKNEELTALKDLLMRRQADFENYKKRV
                     IRMQEDDRKLAIKDMALDVITINDDLIRAIEAASNVQNVENLAEVHTSFVEGVKIISR
                     QIEDMLKRYGVEEIESYNQPFDPEYNEAIEIDESPDVEKDTITKVYQKGFKLDKYVIR
                     AARVKVTRPAKKPDTTSEQTEENTTASPH"
     gene            complement(44312..45355)
                     /gene="hrcA"
                     /locus_tag="PLV81_04240"
     CDS             complement(44312..45355)
                     /gene="hrcA"
                     /locus_tag="PLV81_04240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOM09345.1"
                     /translation="MILKQLNEREAQVLKAIVYEYISTGKPVGSRSFVQKYSFSLSPA
                     TMRNIMFDLEQMGYLSQPHTSAGRIPTDKGYRFYVDSLLDTYETVAKNGVEVREELLT
                     REIELDKIFMSIVRMLSLISKYTGIALMPKPDFTVVKHIEIISLTANEVLFIIVTRTG
                     IVIHKKVRLSSPVNQDDLYKYSRFLTAELSGYSLVEIKQSLINQLRTVTGIEPQVRDI
                     ALDITELALKDEQEPEIYIDGIENLLHIPDMVETETLREVLRFIEQKDTLRKIMEELR
                     QKEGVFTLIGEEIKEIAIPCCSIIASSYKIGNSPVGVVGIIGPTRMDYEKVVPLVDYT
                     GKVVSNFLTQMTK"
     gene            complement(45487..46296)
                     /locus_tag="PLV81_04245"
     CDS             complement(45487..46296)
                     /locus_tag="PLV81_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09346.1"
                     /translation="MPITKAEKAAYNDFIKGYKSKLDELTKQIKETEQKKKKMPNIKG
                     YVNIELILLYTKYIYTLLNMNKASLDMLKIKNERFLNDARKEFYRVLQLFEEIVGKDI
                     DRPLKENEEYLLTIEKLNPSQILNLIKELHTLLQKIMEALGESSKWKWSFVELQGRVA
                     VITKNLINFSDLQKYRDPRKEFYYDRQEMLKLCKQSLSEAAKQYRNKYEISTKSSEDI
                     LRSIELLTALRRINILFGESEEAEKLKNTIEALRLRLEADEKEEEEKKKKK"
     gene            complement(46309..46455)
                     /locus_tag="PLV81_04250"
     CDS             complement(46309..46455)
                     /locus_tag="PLV81_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09347.1"
                     /translation="MEIMRISGADVIPAGFSVNNQQQEEPKVQTEVKKEPEKAKETTI
                     DTYA"
     gene            46869..50078
                     /gene="carB"
                     /locus_tag="PLV81_04255"
     CDS             46869..50078
                     /gene="carB"
                     /locus_tag="PLV81_04255"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOM09348.1"
                     /translation="MPKRSDIKKVMIIGSGPIIIGQACEFDYSGTQACKALKKLGYEI
                     VLVNSNPATIMTDPGMADVTYIEPLTVESLAKIIEKEKPDALLPNLGGQTGLNLSAEL
                     SRQGILQKYGVTIIGVEVDAIERGEDRIAFKNTMNALGIPMPQSEPAYSIDEAKRIAD
                     KLGFPLVIRPAYTLGGTGGGHVYNMEELETIVSRGLSASLIGQVLVEESVIGWEELEL
                     EVVRDAKNQMITVCFIENVDAMGIHTGDSFCTAPMLTISQETQKKLQEYSYKIVEAIK
                     VIGGTNIQFAHDPKTGRIVVIEINPRTSRSSALASKATGFPIAFVSSLLAAGLTLDEI
                     PYWRDGTLDKYTPSGDYVVVKFARWGFEKFKGVEDKLGTQMRSVGEVMSIGKNYKEAL
                     QKAIRSLEIGRYGLGFAKNYNNLDLDELIRLVSTPTSERQFIMYEALRKGADVDILHE
                     RTHIKKYFIEQMKELVELENQMLAYKGKSLPDTLLTTAKQWGFADRYLSKILDIPEDS
                     IRRQRVKLGVVERWEPVPVSGVENAAYYYSTYNAPDKVAVSNRKKIMVLGGGPNRIGQ
                     GIEFDYCCVHAAFAIKEAGYEAIIVNCNPETVSTDYDTADKLYFEPLTVEDVLSIYEK
                     EKPEGVIVQFGGQTPLNIAGELVKAGVKILGTSVDSIDLAEDRDRFRKVIKTLGIPQP
                     ESGMASSLEEAIAIAQSIGYPLMVRPSYVLGGRAMEIVYDEHMLKQYVKAAVEVTPER
                     PILIDRFLDNAIECEADAISDGKDAFVPAVMEHIELAGVHSGDSACAIPPFNISKKHL
                     ATIEEYTKKIALELNVVGLLNIQYAIANDTVYILEANPRASRTVPLVSKVCGIPMAKI
                     ATKIMLGEKLKTFNLKKISIKHFGVKEAVFPFNMFPEVDPVLGPEMRSTGEVLGIANN
                     FGVAYFKAQDAAGQRLPLEGNVLITVNRKDREGALEVAKNFYKLGFSIFATEGTSQYL
                     NEHGIPSTMVLKLQEGRPNIIDKIKNKEINLIINTPYGPSSKWDDSYIRKAAIRYKIP
                     YITTIAAAKAAVNGIEMRKKYGDEIPKSLQEYHAEMKLT"
     gene            50334..>51225
                     /locus_tag="PLV81_04260"
     CDS             50334..>51225
                     /locus_tag="PLV81_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HOM09349.1"
                     /translation="MTLKNTEIQKAEGKLGVLIPGLGAVSTTFIAGVMLIRKGLKLPK
                     GSVAMMGKLRIGKGDDAQYVPIKDFVPLADLKDMELGGWDIFEDNAYEAAVKAGVLQK
                     PDLEPIKEELSSVKPWKAVFFNNYVKKLHGTWVKKAPSYWDLAQMVKEDILNFKKEKN
                     ISRMVMVWCASTEIYQEMKPGVHDTIENFEKALKESNKYIAPSMIYAYAALDLGIPFA
                     NGAPNLCNEIPALRQLAEKNKVPMGGRDFKTGQTLMKTIIAPGLKARMIGVDGWYSTN
                     ILGNRDGEVLDDPESFKTKEV"
BASE COUNT        15568 a        10649 c         8671 g        16337 t
ORIGIN      
        1 tttatttaat actaactgaa tactgtgctg tatttgtctt tttatcatta accattatta
       61 cttcagttac cgcatttgac ttagtttcaa agtaatagta gtttggtttt ggttgcaata
      121 tgttgtttac ttttttatat tcaccttctc gttcatcaag cacccacttt tctataacca
      181 caaggtgtgt gtttggtgat agctttgcct caaaatattt tttttgtgat tccagtccta
      241 tagttgttct tcctgcatct attttatcaa taaatacttt gtaatagcac cgtttatcat
      301 cgtcagggtt ggcaatacta atatcatgtg caataaaacg gaaatttaca ttgacttcaa
      361 ccggagcaat aatcggttca acgggtgatt catatttagg aactgatgtg caggatagta
      421 aacaacttac tgctgccgcc aatactacaa ttacatactg tttaactgtt ttcatggttt
      481 atccctgatt tcaatatgat atataccatc ataggtatca tttaatggct tcacaataca
      541 gtatccctta ccatcaatta tttcatgtac ggttgcagac tttcccgctt taactttagc
      601 tgtttgtaaa atgtatccca atgaatccgt aaccgaaaat gaaacaggta ttttttcagg
      661 tgcagtaaca acaaaatact ttttttgtct tcctttatac tgcaatataa aataatcggc
      721 atctgccttc tttgatacat acccggttat tgaattaccc tgtactgtat tagcctgttc
      781 ttttgtatca ttgggttcaa tatcaatcat atcagatata ttaacaggaa ctatcgctac
      841 tgtataataa ttatcaagat caagctggcc acgttttgcc caaaccttta cataaaatgg
      901 tgtaccaata aaaagattgg ggtgcaattc tttctgtcca gtgctttcat tattcactga
      961 gtacagttct tcaccttttg tgttgtaaat tgcaaacagt atatccagag tcataggtgg
     1021 tgttacttca acacgatacc atttattttc ggaactatcg ccactatagc tatagaaatc
     1081 cacatcacta tctggatata ttctacccat tatggtatca gtcacaattg gtgttgctga
     1141 ctgcagtata ttatttggtt caatctcggt tgtattatcg tagccacgta tggttaccgt
     1201 aacgctatag ggtgtttcat tgtttgctga ataatttctt gcagcaacca taatataata
     1261 gtttcccgca actgataagc caattccttt tgctatctga cttactccct gcccctgtgg
     1321 atttgaagaa aacaacactg taccctgtgc atcaacaata tcaagctgtg attcaatgcc
     1381 aggaacacca gttacctcaa catcgcatac taccggaacc tcttccacat aaaacataaa
     1441 ccaatcctct tcacgattgg gatttaccgc attggtattt gctttgttaa atgcaggcga
     1501 gtagtatccg cgcactggaa cacctactgg tattgggttt gctgataata tagtatcatt
     1561 gggctcagac tcaaagccct ctgcatcttc ctgattgatt aacagtgtat atgcattttc
     1621 agtattttta attcgctgat ctttatcacc atgtgaaacg gaaacatagt atctccccgg
     1681 ctcaacctta aatccccgta atcgttcggg tgctgattta cgcatatcat caacatactt
     1741 caatggcaca ataccatcat caccagcttt ccacacagta aatgaaatat tcacaccctt
     1801 gatggcagaa acgcttatgt caaaaagtac cggctcatta acatctacca tataaaaatc
     1861 aaaatcactt tcagtattaa taaaaccatt aaccggcttg ccaacactta taaggtttgc
     1921 ttgcgtataa gtatcattgg gttcaatttc aaggagtgtt tcttttttgc acgaacttaa
     1981 cagcataatg caacaaagca ccatgcttaa cgataattgt atttttgcca tacacattcc
     2041 aatccaatcc atgattttta tatataatat agtaaacagt ccagctgaat tgttactatc
     2101 tgtgcaatta aagtcaagta tcatttttgg ttgacagtaa atttgtcttg agatttgatt
     2161 tttgaaaatt agcacgttac ttacatcact tcagcgattc tatacattca taggagatat
     2221 gtcatgacca ccaaatcaaa actgtatgtt gcagggattg tattagcaat tcttttgctt
     2281 tctggtgtat atctgtactt taagtacttt ttcacgtatg aacagaagaa tattgtgcag
     2341 cggaaaattg aaacaataac cggacaaaat cttaccatta ccgtatttgg gtatgatggc
     2401 aggataatta agcgctggta cggcgttcaa aagatcacca cacctaaaga tggccgtaac
     2461 tattcttatt tctatacccg tgaaggggaa tatgtacaga tacctgccag tgtgtggtac
     2521 attgccgaag aggagtaatt gtgcttgacc agagcattca atacattaaa gggattggcc
     2581 ccaaacgtgc agagctttta aaacaggaac tgggcattga gaccgttgag gacctgcttt
     2641 actttttacc aaggaaatat attgaccgtt ccaacactaa attaatcaaa gactgttttg
     2701 ttaatgacat tgtgacagtt accggaattg ttaagcaggt aaaaatttca ggccacaggc
     2761 gtaaatttct tgaagttgtt atagacgatg gcagtgatac gttatcaggt gttttttttg
     2821 gcggtattca gtattttctt aaactttttg aagtgggcga cacggtactc ttttcaggca
     2881 aagtggatta ttacactact aagcagatag ttcatccgga gtatgatttt ataggcgata
     2941 gttccgatgt attacacaca ggcaggatag taccaatata cccctcaaca gaaacactca
     3001 aatcgcatgg atttaattca cgggttttta gaaagattat ttacaacgca cttgaattag
     3061 caaagcagca tatccaggaa ccttttgaca gtgagatggt tgaaaggctt aaccttatgc
     3121 cactgtcaca ggctattcag cacattcact tccctgccac aatggatgaa gcacatcatg
     3181 cacgtgtgcg attggccttc aatgaacttt ttttcctaca atattattta catctttcac
     3241 ggcactatat cagagaaaca cataaacatg caaaacctcc tataaacgat tctctgtata
     3301 gcgattttat ccaaaccctg ccattcaaac ttactgatga ccagataaag gctgtgcagg
     3361 aaattattaa tgatttacaa gcagattttc ccatgaaccg tttgttgcag ggcgatgttg
     3421 gttctggcaa aacgatagtg tctatgattg caagccttgc tgttataagc cgtggacagc
     3481 aggttgcctt catggcacca acggaaatac tggcacaaca gcattatgct acattcaaaa
     3541 agtttttacc agattctgta actgtacagc ttcttactgg ttcaacacct gctgccgtac
     3601 gcaaagaaat tcttgcaggc ttaaacaatg gatctatcaa tatcatcatt ggcacccatg
     3661 ccctgattca ggaacacgtg caatttgcaa acctgggatt tattattatt gatgaacaac
     3721 atcgctttgg tgttaatcag cgcgcaagtc tcagaaccaa aggctatatg ccagatctgt
     3781 tagtcatgac tgcaacgccc attccacggt cactatcgct aacattgtat ggtgatctag
     3841 acatttcaat aataaaacaa aagcctgcaa acagaattcc tgttacaaca cttgcctttc
     3901 catcatcaaa acttgaagct gtgtatcgct cactggaaaa atatatgaat gaaggcagac
     3961 agatatatta cgtactgcca ctaattgaag attcagaaaa actggattta aaatccgcaa
     4021 cagcaaccta tacacagctt gtgaagcgat tcccaacaaa aacgattgcc ctgttacatg
     4081 ggaagcttcc caatgaagaa aaagaaaaaa ttatgcagca atttaataat cacaccattg
     4141 accttctggt cagcacaact gttattgagg ttggaattga tgttgccaat gcatcaatta
     4201 ttgttataga acatgctgaa cggtttgggc tttcacagct tcatcagtta cgtggtcgtg
     4261 ttgggcgtgg tgaatacaaa tcgttttgcg ttttaattta tcctgatgat atacctcagg
     4321 aaagtatgca acgcatacat attttaacaa caaccgatga tggctttgtt atagcagaag
     4381 aagatttgaa aatgcgtgga gcaggtgaat tcattggcac ccgtcagcac ggacagggta
     4441 tagcgtttga atttgcagat cccgttaacg attttgaact tatcagtata gcccgggaag
     4501 aagcacaaaa gcaggtagaa agattaaaag atattcctgc gctttttgaa aatattaaac
     4561 gaacacaggc aatgaatata ctggaaggat taagaaaaaa acatatactg gcactgctat
     4621 cgtaatcagc ttatctaccg caacagtttt tatatttctt gccactgcca catgggcaag
     4681 gatcattacg accaatttca gttccggttt gttgctgaat tgcttcaatg atattcttat
     4741 tattcatttt ctgtaaatta aagattttag cgtgttcctt taatgcaatt tgagtatccc
     4801 acattacccg tacaaagcct gttaattttt tcaggaaagt agtatcactg gcaaaatcct
     4861 cctgtgacca cttttcttcc atgctaataa ttaaagcttt caggagatta acattgccaa
     4921 taaatattga atatatttta ttcacttttt cgtttttcag catttcatct aattggcctg
     4981 tggataaata ttttaaaaac gtttcagcac gcatgatgga agcattcaca tacgctgtat
     5041 atgcttcgcg ggttttcatg tcaaaatccg gctgctcaag ttcactgtca tcaaatcgtt
     5101 gcaatcgtaa caggtaaaac cacaatgact caatctgatc tgccacatct ttattttcca
     5161 gcacttcttc actaaaaaaa tcttttataa gtgaccgcaa cgtaaaactg cctgctgaag
     5221 cctgtactcc taaaatagca gctttaagat tatatatgtc tagaggtata ttattcttat
     5281 ccagaatctt tttaagattt tcatcacctt tatattgttt aactgtgaac atagtaatcc
     5341 ttcaccctac ttgaatattg ttctatcaac aaagcggtta aaaattaata tttgtttctg
     5401 attataaacc tgtaaggcct tttgttttat aaacgatttg cggtccagct tttttaatgt
     5461 ttcggggttc aatgttccaa gatatgcatc aatttttgca acaaactcgt ctatctcaga
     5521 aaaatatttt tttgaaacct gcctgcgaat aagatcaaga gcattaacac tgtcaaatgg
     5581 caaatctctg ttattgacgt tattatcaca ccattctaaa acaattgtct ttaaaaattc
     5641 atatggaatc aacttcttga ttttcttgcc atcccttgaa ctgaaacagt acccttcacc
     5701 tgatatctgg atatcagcta aaagcaaacg aaatatggta tgtggcccta aatgataata
     5761 aaacaaccat tgatcatttt caggaatatc aaaatattca acactttcat gcaagataga
     5821 taaatttttt ttactgataa actcaataaa atcttcaaca atatcatact tttctttatc
     5881 gatagatagt tttaataaag ccctccgtaa ttctgatttc atctggtcat cagtcaattg
     5941 tgttttggta taatagacca tatactcaag atcttcctgt tctatcttca atacgttttc
     6001 atcaaccgat agaatatatg gttcacgagt attccatggc tttgtgcctt taactgtaaa
     6061 tggaactgta ctttgctggg tactgtattc agattgagtt ttacttttgc ttatttcata
     6121 ttgtttttgt ttattatatt cattggaatc ggaagcttct tccataccat caggagaagt
     6181 taacgttgat gttttgtgga tattatgctt taaagcttct gattgatctt gtacgaattc
     6241 agaggtgtcc agttcattta acaaactttg cactgtatat gatttaaatt cattaaattg
     6301 caatttggaa cgtatatcag catcagacag attataatga tgagaaagta accctaaatg
     6361 attagcaatt aattccagta acacatctgt agctttacac aggctgtttc cttcaacaat
     6421 aatatcatta agtactgatg aaagctgttt gccgccctca gcaaagttaa acactgcaga
     6481 ttgcttttca agctctgtcc aatctttttt aacaatacta ttaaatgaac gcaataacaa
     6541 atatatttct ttaagaacta taaagtcaag ccggttgtcg tcaggataat catcggggaa
     6601 ataaagggga aaatctttgg ttgcttttcg tcttaaattt gcttcgttta tttcaccaat
     6661 aaatttatcc gtaagaattt caatatcttc aatagtttta aaaatggagt aagacgattt
     6721 ctttattacc ggaacaatct gttcaggaac ctccgttata tccaaacgtg accgtaaaag
     6781 ctttgccaat tcaatatgcc tgtgtatccc actggacagc ccgcgcttaa aaaattttac
     6841 cacttctgat atattttttt tctcaggtaa tggtatatca tgtattgttt catctagttc
     6901 actatcaagg acatgttctt taaaatgctt tgcatgtgct ttctgagctg tataatcctt
     6961 atcactatca atatcctgca ataacctgtc cagttcatca tctatcatac aaaccccaaa
     7021 cactttatgt tggaatcact tctttatcat ccagcaaagg tttttgcgat gctttctttt
     7081 ttattttttc tattttaggc tcttcgtcag gctgttgtgg attttcgcca ttatgaatag
     7141 gcggtaatgg ttcacctttc attaatagtt caatttcctg agcattcaga gtttcacgtt
     7201 ctataagatt tgttgcaagc aattcaaatt tatcactgtt ttcttctatc agttttatag
     7261 ccctgtccct gcaactggtg acgatatcat gaacttcttt atcaataagc tgtgctgttt
     7321 cttcgctgta atctctgtgc tgagctatct cacgagccag aaaaatctgt tctgtttttt
     7381 tgccaaatgc taacgggccc agtgtttcac tcatacccca ttcacacacc atgcggcggg
     7441 caatatcagt agcacgttca atatcattgc ttgaacctgt tgtaacctgc ccaaatttta
     7501 tcttttcagc aacgtggcca ccaaataaaa tggtgatctg attgagccag tattctctat
     7561 tatgcaagtg gcgttcttct ttgggcaaat gcatggttaa gcctaacgcc cttccacgag
     7621 gaataacagt cactttgtgc acagggtcgg ctccggtaag tatacccagc aatgcatgcc
     7681 cagcttcatg ataggcaatg accttttttt cttcggcaga aatgaggact gactttcttt
     7741 caggacccat caatactttg tccttggcat cctccatctc gagcatgctc acgcgctttt
     7801 tattgcgccg tgcagcaagc agtgcagcct cattaacaag atttgccaga tctgcaccag
     7861 taaatccagg tgtaccacgg gcaataactt ttaagtctac atcctttgcc aaagggattt
     7921 tgcgggtatg aacagctaat atcttttccc ttcccttgat atcaggtatg tcaacgacaa
     7981 cctgacggtc aaaacggccg ggcctgagca acgcagggtc aagtacatca ggcctgttgg
     8041 tagcagcaac aataataaca ccttcgttag tttcaaatcc atccatctca acaagaagct
     8101 gattcaacgt ctgttcacgt tcgtcatggc caccacccag tccagcacca cgaagccttc
     8161 ccacagcatc tatctcatca ataaatatga tgcagggagc attacgcttt ccctgatcaa
     8221 acaggtcacg tacccgcgat gcaccaaccc caacaaacat ttccacaaaa tcagaaccac
     8281 tgatggaaaa aaatggcaca ccagcctcac cggcaacagc ccgtgcaagt aaggttttgc
     8341 ctgtaccagg aggccctacc agaagaaccc ctttaggtat ctttgcacct atggtaataa
     8401 atttcttggg gtcttttaaa aattcaatga tttcctgaag ttcaacttta gcttcatcaa
     8461 cacctgctac atcggcaaac gttactttat ttttagtttc aggctgtaat ttagcccgta
     8521 ctttgccaaa actcattgcc ttgtttccgg tagactgtat ctggcgtatc ataaaaaacc
     8581 agataaaccc aaagaaaaga agccacggca gaaaattgag tacacctttc cagaaaagat
     8641 tatcatcaac ctcttcgcct ttaatttcaa cccgattatt aataagtgtt tttattaaat
     8701 caggatcctg atatggaata accgtctcaa aaagatagac tttgtcgcct ttggtaaagg
     8761 ttccttcaat ttttttgcca ttaactatcg taactgattt aactttattt tcatatactt
     8821 tctttatgaa atcactatat tgaagctgct caactttctg agtattcata tcattcattc
     8881 tgaatatggc gatagcaagt agcgctataa gcaaaagcag agcaacctgt ttagcagctt
     8941 tattcataga atcacccgtg attatctgtt ttgatcaaga agttatacat atattttact
     9001 gtagtcttta cttacaagat actacaaata cagaaatact caaataaatt tattgtacca
     9061 aatccaactt ttatcaatta gccggataac agacatttca gtttaatcaa gaggcatttc
     9121 atacattatt gctatgccag taacgaataa caagcatttt ttgaccattt tccggcaaaa
     9181 aagaaggtga aacttcagct tttatggcac ctgtaacatc aaataataca gcagctatct
     9241 gattatcgat tacaatcagt ggcaccagat ttttttgttc cggagtaagc cggtaatcaa
     9301 tatataagcg tttcagtgtc cgtttacccg ttttacggac aattgcatcg ccagtccgct
     9361 tattcctgat aacaatatgg tcacatccgg tatagcgaag tattagagta ccgctttcag
     9421 atgattgcac atcaattgat tcagcaacag cgcattgaac ggtaataccg gcctgtgtaa
     9481 cagttacagt tttccccacc ggaagattat attcatagtt actatcgccc atacataaag
     9541 gctgtgaaga aaaaattaaa aacgatgtgg tagtgcgata gcgcctgtat atgtagattc
     9601 cttttccttc aaaaagggtg atattctttt tctttgaaaa atgcgcacga tacagtgcat
     9661 ccagaatatc agaatcaaca tattttttga gatagttact gataatatat gcacaataaa
     9721 atttaaatat atattcactg tgtgcaatgg cattttcctg cacctgtatt aatccaggtt
     9781 gctcttgaat aaatcctgga tacagcgtat gcatcaggtc attgatgctc cgggttgcat
     9841 catgtgcctg tttctgaaga ttaagcagtg cttcatcaaa gtgtgggaac cgcttttgaa
     9901 tgaccggcag tacactgtgg cgtatatagt ttctaagata tgtagtatca tcattggtac
     9961 agtcatgccg gtaagataag tcattatgtt ttagatatgc atacacctca tcctgtgaca
    10021 gcaccagcaa aggacgtatg atgattccct gtgccagtgc tattgcttct aaaccctgca
    10081 atcctgttcc ctgaaacata cgcataagca gtgtttcaat ctgatcattt tttgtgtgcg
    10141 cagttgcaat tttactatag ccatagtgtt gggctatcat gtgtaatcgt gtatagcggt
    10201 attctcgtgc ctgctgttca aaacttttcc cgggttcttg atgttttgca aaatcaaatc
    10261 gttcaataaa agccgggatc ccataccgtg aagcctgatt catgacaaat aaaagatcgc
    10321 caaacgattc ctcacctcgc actttatgat caagatgata cattgcaatg gtgcatccaa
    10381 ggtgctcacg tagcatatac agtacatgta atagtgccat tgaatccttg ccagcagaca
    10441 ctgataccag tactctatcg ccaggcacaa taaggttatt atcctgtata aatgtataca
    10501 ctttttgtac aaggttattc atataacggg agttataata atcatttcat acatatcaat
    10561 aatatatccg ttataagcac tgtggggatt cttgcaatat atttttatgt caggcactca
    10621 gtgctttttg cactatcgtg gggtgtggtc cccaataaaa tttacaaaaa aatatgtttt
    10681 cttcatcccc aatttcaaaa gtataaccac tatagcgata gttatcggta tagtaatctt
    10741 catcttcttc agcttttgct aaaaattcca aaaattcata caatacatta tccagacaat
    10801 atgcaataac ctttgccaat gacatcttcc atagcttctt cacatcaaga aaaaactcat
    10861 actcatcatt atataatacc agatgcaatc tcttccactc cccttcgtta cgtgttctgt
    10921 attgtatttg tttaaagtac tgtatatcac ctttttcaca caaagcagca aaacttatta
    10981 ttgaggatac aaatgtccta agcgacatgt ttttcttttc tgcataaaat ttcaatattt
    11041 caagatgttt gtatgaaatg cacgtggtgg tttcaatata catatgcttt cccttccaaa
    11101 gtagttttta ttttttatat ttgattttat cgtaataatt tataataatt tttcaagcat
    11161 aaaataatat tttgaaaaat caaatataaa ctaacattag gtgtcaactg aaatctatat
    11221 taatttttta ctaaaacatt tagaattttt ttattgccac agtgataata acaaataatt
    11281 tttgtacatc ctttacatcc ataattatgc tattgacctt gcaggtatta tagtttaaaa
    11341 tagctcaaag ataatccgtt ttataaggag atagttacca tggacatcac cattcaaaaa
    11401 cgaatagaac aatggcaaaa tccaccattt gataccagca ctatacagga gataaaacaa
    11461 cttgttgcac aaaataatga aaaagaactg tacgaacgct tttataccac acttgagttt
    11521 ggcacaggcg gacttcgtgg aatcattggt gctggcacca accgaatgaa catttacacc
    11581 gttggcatgg caacacaggg cctggcaaac tacattgtac accagggaaa tgctgcaaaa
    11641 ggtgtagtta tagcatatga ttcacgcagg atgtcagatg tattctcaaa agaagcagcc
    11701 tctatattag cgggcaatgg gattaaggta tatcttttca aggatatcat gcccacacca
    11761 atctgctcat ttgcaatacg ccattacaat gccacagcag gtattgttat cacagccagt
    11821 cataacccac cagaatataa cggatataag gtatactggg aagatggcgg tcaggttgtc
    11881 ccgccccagg ataaagaaat aatacgtgaa gtttcaaaaa tacaggacat cacacaaatt
    11941 aagaagatga attttgatga agcgatagct catggcatta tattgttagt tggcgatgaa
    12001 atcattgaaa gctatgccca gaaactttca caggtacgat tcaaatccga tgaactatcg
    12061 ccaatacaca ttgtatacac accattacat ggtactggtt accgtattgt tcctcaaata
    12121 ctcaaaacat ctgggttccc caatgttcat atagttgaac aacaggcaat ccctgatggc
    12181 aacttcccca ctgtcaaatc acccaatcct gaagaacctg aagcgcttac acaggccatt
    12241 gaacttgcac aacaaatcaa tgccgatata gtgttagcaa ctgatcccga tgctgaccgc
    12301 atgggcgtag catttaaaga tgacaatgga caatatatgc ttattaatgg aaatcagata
    12361 ggcagcatgc ttttgtatta tatactgtta cgattgcagc aactaaacaa gctacctaaa
    12421 aatggatttg ttgtaaaaac aatagttacc actgacctgc agcaggagat agctcgtcat
    12481 ttcaatgtta ctattgaaaa tgtcctcaca ggatttaaat ggattgcaaa aaagatgaaa
    12541 gattatgaaa agtcacatgt atttattttt ggtggtgaag aaagttatgg atatttgcct
    12601 cttgattttg tgcgtgacaa ggatgcagta agctcctgct atttctttgc cgaaatggca
    12661 cactggctcc attcacacaa taaaacactg aaagactttt tatatgaaat atacactcag
    12721 tttggctatt acaacgaaaa gcttgtttcg cttacactga agggtgtgga aggcattcaa
    12781 aagatagaat ccatcatgaa gcactttaga agtaatccac ccaaacaact ggccggaact
    12841 actgttactg caattcttga cgtcaaacag ggaacgctta ctgatgtaat gagtggtacc
    12901 acaaaagctg tggatttgcc tgcatcggac gtgttgcagt ttacgttaga aaatggctca
    12961 aaggtaacca tgcgtccttc gggaactgaa cccaaaatca agttttattt cagctcacgt
    13021 aaaaagggaa cagacatcaa acaacttgaa gaagaagtat cacggtataa tgaaacgtta
    13081 gcaaaagaat tattgagtac aattgaatcg gttattacat aactacaaag catttatact
    13141 atgctttaat ctacaaattt ttgttgccat atccacaacc acaagatcta tgaatttacg
    13201 gtttagtggc actgaacatc ttgtatgggc aaagccactg cactttatta aagtgtagca
    13261 atctcatcct cacaaaagag attgcctgac tttgcttgca atgattatgg tgagttaggc
    13321 aggagtgctc atcaaatctt ttgcataatc tcttcttctt ttttctgcat ttttcttttt
    13381 ttgtataacg attgttcatg gtcatatccc aacaggtgca gcactccatg taccagtaat
    13441 ctctttaatt cttctttcag ggtcactcca tattctatag cctgcctttg ggcagtctcc
    13501 agtgaaatga aaatatcgcc taaatgttcc tttgtggtaa catgtggcat gggtgcttca
    13561 cggtaggcaa aagatatgac atcggttgga cctttttttt tgcgataggt tttattaata
    13621 tgagctatcg cctcatcatt agttaaaatg agtgtaacaa ctacattatc aacttcctga
    13681 ccctgtaaaa cagcagctat aatttttttg caaaacgcgg ctaactcttt aggcatcgtg
    13741 atgtgctcac gttcaacatt aaccttgatt gccattagat gattttttct ccaatcggtt
    13801 tattgcttcc tttgtggggt attcaatacg tgaatggaat atgccctgta acacctgcac
    13861 aaacgcattc tgaattttgt ttaattcatt cagcgtaata tcggcccttt ccaggtcacc
    13921 ttcattcagc ctattataga ttattcgttt taccagtgtt tccaattttt ccagtgttgg
    13981 ctcctgtatt gaacgcgatg cagcttcaat tgcatccgcc agcatgacaa ttgcggtttc
    14041 acgtgtatgt ggttttgggc caggatactg aaaatcactt ttctgtatgc tattcccgtt
    14101 agtggtgcct tcttcaagtg cctgatggta aaaatatgtc atcactgttt gtccgtgatg
    14161 ctcacggata aaatcaatga ccatttcagg aagattattt tttcgtgcca gttcaacgcc
    14221 cttttcaaca tgtgagataa ttaatctaca atattcaagg gctgacatat ccttagcacg
    14281 tgggtcggta accttattct caatataaaa atttgcgtcc tgaatttttc ctatatcatg
    14341 gtagtacccg ccaacacgtg ccagcaatgc attagcacca acctccttgc atgcagattc
    14401 tgcaagtgtt gccaccatca gtgaatgatt ataggttccg ggtgccttga taagcatctc
    14461 tttaaatatt ggcgcattga ggtccgatag ttccagcaac ttaaactttg ttgtaacacc
    14521 aaagaggttt tcatataccg ggaacacacc aaagacaatt attgcattaa tgatacctga
    14581 tataaaagcc aatccaacgt tatttgcgat gatgccaaat tgcatttcct gcatcaaccc
    14641 cacagcaatg actattaccg aattaataag ccctaaggta acaccaccac gtaaaaaatc
    14701 agaacgacgt tcaacatcac caacaacaaa tacacctgat aatgcggaac tgaatgccaa
    14761 tagtaatgtg gtaaagtcgc ctttcatgct taccgttaca aagaacacaa tatacacacc
    14821 aatcaacatg gcaatgtgca tattgtacat gattgcaagt accatcgtga ccaggggaat
    14881 aggtaaaaga agtgggaaga acaaaccctc aaaagccctt ccctgccacc tggctataaa
    14941 aaaggcaata acaataaaag ccagaacaag ggtaaacact atcagaggca tcttattatc
    15001 aacaaccagg ttgctataat aggttatgag aaaatagcca aagatgacta aaaatcccaa
    15061 ctgaataaga agcagcccta taataaaact tgatccagag aagtgcgcat tcttattaat
    15121 aatattaatt ttttgcaaca tatcctctgt tatggtatca ccttcacggg ctattgactg
    15181 gcctttcttt atcacccggg taactggttt taccgactgg acagcttcag ctattcttct
    15241 ttttgtttct tcctcattat aaaaaagatt tggctgcaac agactgataa taacagaatt
    15301 tactgcatat aacgcatcac tggaaggttt gttaagaata tctttgctta tgactggcac
    15361 cagcgattgt atttttttca tatctactaa tgcatcaaca ttcaactgtt tttcttttag
    15421 ttctgaagca cttcccacta ctctcacaat gacatgattt gactgtaatc gtaatggatt
    15481 ggtatatggc ttttccagaa taccattatt atatatttgt aataaaattt tattcaggtc
    15541 ccggtgaaga agctgttcat catattgcaa caatcccatt aataccctat ccgaatactg
    15601 cagataatca ggcaacattg tcctcagcaa caacacctgc tttgaagggc tactatcggg
    15661 catttgttct ttaactttaa ttactgcctc acacagcatg ttgcttattt taagcttatc
    15721 ctctacaatg gattcattga ggtcaaagat tgcaggcatt gcttcggcaa ttctattctt
    15781 ttctttttca gtttctatag ctacgggata ttcaatgttc atgggtgctc taatatcctc
    15841 acgggctata tccccagcct gataactgta tgtagcacct atcctgttta atgtaagaag
    15901 ggcagtagcg gtaataataa ctatcaatgt ataaataaaa gaagctttca ggcttttctt
    15961 cttgaaagca aactgtatgc cccttttaaa aaccataaat gaatttaaca tatcagtaac
    16021 cggattgtat tattcagcat gtttttcata cgctgcaata attttttcaa ctattggatg
    16081 ccggcagatg tcctcttttg agaattcaat gaaggtaatc tcttcaatgc catgaagaat
    16141 cttttgtgca gcaagtaaac cagattgacg tggcttttcc agatctatct gagtaatatc
    16201 accagatata accatttttg aactgttacc cagacgtgtt aaaaacatct tcatctgtga
    16261 tgaagtggta ttctgtgctt catcaagtat aatgaatgcc ttattgagtg tacgtccgcg
    16321 catataggct agcggtgcaa tctctataat ccctttttcc atatacttgg aaattctttc
    16381 atagggaaga atatcaaaca gggcatcata cagcggcctt aggtatggat tgatcttctg
    16441 aataaaatca ccgggtaagt accctaaact ttcacctgct tcaacagcag gccgtgttaa
    16501 aataatgcgc tccaccctgc ccgttaacag gaactttaca cctacagcta ctgccagata
    16561 tgtttttcct gtcccggccg gcccaatgga gatagtgatg gggcttttat ggattgccga
    16621 catgtaattt gcctgattga ttgtcttggg tacaatggtt ttccccgttt cagggataac
    16681 tatctttagt ttgttgattt catcagcatc tactttaatc ccctgattaa tagaaagtgc
    16741 caggtagcgt atatcaaaat catcaaactc atagagggaa tcacgcatat ataaataatc
    16801 acccatcagg tgcaaaagct gtaaagcttt agcggcatta tcaccggaag cttcaactat
    16861 cagggtattg ccacggggga ttatcttgac atcaagaagc ttttcaattg ctttgatatt
    16921 cttatcttta tacccacata tgcgtttgta aatagatata tcatcaattt caaattttgt
    16981 atctaccatt ataattcaac caccttgata gatagctctt ttaactgctg tgcactcaca
    17041 ggcgaaggcg cttcactcat gaggcactgt cctttttgcg ttttggggaa tgcaatcaca
    17101 tcacgtattg aattgcgttt gagtaacagc atcaggatcc tgtccaaccc cagtgctatt
    17161 ccaccatgag gcggtgcacc atattttaat gcatccagca aaaatgaaaa ctttattttt
    17221 gcctcttcct cggaaatacc aaggatgcca aacatctttt tctgtaatgg catgcggtta
    17281 attcggatag aaccaccgcc aatttcaaca ccattcagta caacatcata tgcctgcgcc
    17341 ttgatgtctt gagttgtttg cggagtaaca gtatcaagca ttggctcgca ttcaggtttt
    17401 ggcgctgtaa acggatgatg cattgcatag tagcggcctt cctcagcatc atattccaga
    17461 agaggaaaat caaccaccca caggaagttg agtttatttt cgtcaattaa attaagctgc
    17521 tgtgcaatct tgatacgaag attgcccagt gcatcattga caatctttgg cttatcacag
    17581 gaaaaaataa tgagtgcatt ggaattgagt tttaacctgt caacaagctt ttgcttttca
    17641 ctatcgctta aaaattttgc tatccctgtt tcaaaagcac tccccgtata acgcacactt
    17701 ggcagcccca ttgctttaaa cgtgcgcaca taattaatgt attcatcaat cattgcccgt
    17761 gaaattttgc cttcatcgtt aataactatc gccttaatta tgccaccatt ttccagtgct
    17821 gccttaaaaa cattaaacgt tgaattggta aagatatcac ttacatcatt cagctcaagt
    17881 ccaaaacgtg tgtcaggggc atctttgcca tagcgctgca taacttcatc atacgtgaat
    17941 acagggaacg gcgtttcaat ttctttatcc agcacctctc taactatctc tttcaaaagc
    18001 ccttcaataa tttgcataac catttgagga ttaacaaatg acatttctaa gtcaacctga
    18061 gtaaattcag gctggcggtc attgcgcagg tcttcatcac ggaagcattt ggcaatctga
    18121 aagtagcggt caaaaccgga aatcatcaaa atttgtttga aaagctgtgg cgattgcggc
    18181 agtgcataaa acataccacg gttaatacgg cttggaacaa gaaaatcacg cgcaccttca
    18241 ggagtggact tattcagtat aggcgtttca acttcaataa accggttatc agtaagatat
    18301 cgccgtattg tctgcataac cttatgacgt actataatag cttcctgcat atcagccctg
    18361 cgaagatcta aataccggta cttcaaccgc acctcttcgt tcaggttagt ataggtatca
    18421 atgggaaatg gtgtcacctc agactctgat aaaatggaaa tatcctgccc aataacctct
    18481 atcatgcctg ttttcaaatt gggattgact gtttcatcag aacgctttgc aacctggccg
    18541 gttacaaaaa tacagtattc atttttcact ttatcagcat tttcatacgc ggtactattc
    18601 ctggaggcat caaacacaac ctgtacaatt ccagtcacgt ccctcatttc aataaatatt
    18661 atgccaccta aatctcgctt ggtatcaacc cagccagcca aacgcaccat tgtaccaaca
    18721 tcattaactg ttaactcacc acagtagttt ctttgttcaa acattcccct aacccaatag
    18781 aaaataatat agcaacatac cagaatcaat taaaaattca aaaaaatatg ggaattttgc
    18841 tccccgcctg aagcagtttc ccgccaacag gacacagtag caaataattt gatttcgata
    18901 gtattacgga ggcaggctga tttttgtcaa gtaattatat gcacatccat tatttcattg
    18961 ttttgaacgt gtttacaatg gcttcacact gtttttttga cttcccttca gcaatgattc
    19021 tgaatattgg ctcagtattt gaaggacgta aatgcaccca gccaccttga aacgcttcat
    19081 gctgcacaaa atctatgcgt ataccatcaa ggatattaat aacttcatca gtatactgtt
    19141 ctttaatttt ttgggcgata gttccggtat caaattcacc gcttatattc atgacatcct
    19201 tgtgcatata atacgcaggc agtgatgcaa caatctgtga aattggttct tttctgtgtg
    19261 ccattagctc cagacaatac ccgataccca ccaggctatc cctaccgtaa tggacctcag
    19321 gcgaaataac accgccgtta ccttcaccgc caatcactgc cctgtattgt ttcattgctt
    19381 caaccacatg tatctcacca acctttgagc gatacactgg tgcaccaaac tgcaacgcaa
    19441 catcctcaat tgctttcgtt gtggacatat tcaccacaac aggccctttc ttcattgcaa
    19501 gacaccgcaa agcagccagc gtcacggtaa gctcctcgcc aatgggattt cctttttcat
    19561 taactatcgc caacctgtct gcatccggat cctgtgcaaa gccaatatcg gccttatgtt
    19621 ctttcacagc ctttgataat gcttttagat tttgtgcaac aggttctgct ccccggggaa
    19681 atgtaccggt agtcatatcg caatgaatgg gtattaccct gcatcctaat ttcttcaaca
    19741 gttcttgagt aatgagtgaa ccagcaccat taaccgaatc cagtaccacc gtgaattttc
    19801 tttttttgat agcggcaacg tctattgcag caagtacttt ttcaatatgg acgtttgccg
    19861 cagaactatc gcacataaca ggctttatgg cattccactt tgcaccaaat ggcgtaatgg
    19921 tatcaacaag tgataaaaac gtattcatct cgtcaggacc aaagaagcta ccagttgcat
    19981 tgataagctt gaaagcattc cactgtatgg gattatgtga tgcagaaata accacaccac
    20041 ctttggcctt agtgtgctcc accataatct gtacagtagg cgttgggacc acaccaatat
    20101 caatgacctg gcatccacat agtgacagca cactttcaaa acattgtgca atctgctgcc
    20161 cggtaatcct ggagtcacga ccaactataa cgttgccgcc atgaatatgc ctggcaaatg
    20221 ccattgcaac cctggtaatc aaatcaggtg taaaagattc cccaaccaca ccccgaatgc
    20281 ctgaaatgct tttcattaaa ctcatagaaa cctctttata cactacatag caaaatcgtt
    20341 atctaacacg tattgacgcc tgtatagtta accccgtctg cttcttcttg tttttttgcg
    20401 cttcacaccg cctgttattt ctctggtttt atcttcctgg ctgcgtattg atggccgtgg
    20461 tatagcagca acaggaattc caccagcacc aaattgttca acctccggtt ttatttcctg
    20521 cccaaacggt actggaagct cttcaggttt aatttctatt tcctcaacct tctgtaagcg
    20581 cactttcaga agcatctcaa tgatatctac ttttagatta tcaagcatct cgttgaaaag
    20641 ctcaaaaccc cttagcttat actcaaccag cgggttacgc tcactgtagc ctgctgtcca
    20701 tataccatca cgcaggtcat ccatgaccag cagatgatca cgccacttgg tatcaatcac
    20761 ctgaagcgat accagccgtt caagcgtacg catatggtcg gttccaactt cttcttcctt
    20821 tgctttatat agtttcagca attcttcttt tacacaatca ataagttcat catgcgataa
    20881 accctcaata tctaccccta acgatccaat atcaaatcca aactttgatc taagaaaacc
    20941 attcaatcct tcaatatccc agtcttccga atgacgtttg ccttctgtaa aataaggaat
    21001 ccagctttcc accacatcat caatatactc acgaattttg ggcgaaacat cttcaccatc
    21061 aaggattgca ttgcgttcct tatatatata ttcacgctgg ctgttcatga catcatcata
    21121 ttccagcagg tgcttgcgta tttcaaagtt tcttgcttct actcttttct gtgcattttc
    21181 aatggcacgc gacaccatcc ggctttcaat ttcctgtcct tcctccatac ccaaacgctg
    21241 cataacaccg gaaatcctgt ccgagccaaa aatacgcatc aggtcatctt caagtgaaag
    21301 ataaaaccgt gatgaccctg gatcgccctg ccttcctgaa cgcccacgta actgattatc
    21361 aattcgtcgt gcctcatgac gttcagtacc cagaatgtgc aatccacctg cctctaccac
    21421 tttattgtga ttatcaaacc attccttacc tttacgtaca atatccagag ctttgtgctg
    21481 atcctgtcct accatggtat tggcaaggct tgctgcctct tcaaagttac tttttaaaat
    21541 tgcttccctg aagcgttccc atagtgttgc atcatgaatg ggcttccagt tctccagttc
    21601 atcaatatac gtctttctgc caccaagcac aatgtccgta ccacgacctg ccatatttgt
    21661 ggcgatagtt actgtaccag ggcgtcctgc ttcagcaata atctgggctt ccttttcatg
    21721 atacttcgcg tttaacacat tgtgcggaat gcccactctt ttcagcatgt ccgataattt
    21781 ttcagatttt tcaatagaga ttgtacctac cagtactggc ctgccctctt cccgcaattg
    21841 ctgaatttcc tgtactatag cattaaactt ttctttttcg gtacgataga tcctgtcagg
    21901 ataatcggtt cttcgtactg gttcatttgt gggaatgaca acaacatcta agttatatat
    21961 ctttttaaat tcgatagctt ctgtatcagc tgtaccggtc ataccagcta acttcttata
    22021 catcctgaaa taattctgga acgtaatctg tgcaaatgtc tggctttcac gcgctattgg
    22081 cacattttcc tttgcttcca gtgcctgatg caatccatca gaatagcgcc tgccgggcat
    22141 aagccttcca gtaaattcat ccacaataat gacctgcccg tctttcacaa tgtaatcaat
    22201 gtcacgtttg aataaagtat gagcttttaa tgcctgatta acatgatgca ccagttctat
    22261 attgcgacta tcgtacagat tatctatctt taaaagtttt tcaacatgtt tcacaccttc
    22321 ttcggtcaaa agggcattgc ggtctttttc gtttacttca taatcaacgc cctctttaag
    22381 gcttggaata attttattta tcaggtagta tttcttggtg gattcatcag tggaacccga
    22441 gatgataagc ggtgtacgcg cttcatcaat gagtattgaa tcaacctcgt ccacaattgc
    22501 atagtaaaga tcacgctgca ctctgaggga tttatgttct accatattat ccctgaggta
    22561 atcaaaacca aattcattgt tggttccata ggtcacatca cagcggtaag ctttctggcg
    22621 tgcgtaataa tccatattgt gctgaataac accaaccgta agtcctaaat atttataaat
    22681 tggacccatc cactcagagt cacggcgagc taaataatcg ttaacagtaa caacatgaac
    22741 ccctttaccc gcaagtgcat tgagatatac cggcattgtt gcaaccagcg ttttaccttc
    22801 acctgtcttc atttcggcaa tctttccctg atgtaaaaca atggcaccca taagctggac
    22861 gtcaaaatga cgcatgccga tggtgcgcca tgcagcctct cttaccatgg caaacgcata
    22921 cggtaacagt tcatccattg gctgaccctg ctctatgcgc cctctgaact cagcagtttt
    22981 tgccagcatc tggtcctgag ttaatttttt aacctcatct tctagtgcat ttatttgagc
    23041 tacaatgggc tgtagctttt taatatcacg ttcgtgcttt gtaccaaaaa acaaacttaa
    23101 aattttatct atcatatcat ttacctgttt ataaaattat gatttactat ctgttacata
    23161 actgttaata caagtatttt ttcatgagtt acatgtattg tatctaacgt ttttattgta
    23221 gaaacaacta tatatctgtt gttacataaa agcatgtaat atacttcttc ctcaaccatt
    23281 aaaaaacaat caaatcatcc ctgttaataa cctcttcata gtattttgaa ccaaatattt
    23341 tttttatctc ctgagtgttt ttgcctttta ttttttcaag ctctccgctg gtataattaa
    23401 caataccttt tgcaatcagt gttcctcttt tatcagtaat ttctactgca tcacccagat
    23461 caaaattccc ctctatttgt gttatgccta ttgccagcaa ggatttttta ttttccttaa
    23521 gtgccttaac agcaccatca tcaataataa gctttccttt tggctttaca ttgaatgcta
    23581 tccagcgctt tttgcttttc aattgcgttt tacctaaaaa cagcgtacct atcttttcac
    23641 cctgcataat tcgctgtaag atatgttctg tcctgccatt cgcaataatc atcatctcac
    23701 cggattgtat gaccatttct ccggctttta tttttgtata cataccgcca gtgccgtact
    23761 cactgccagc acctctgcag cgctttcgca cttcatcatt aatttcatga atttctgata
    23821 tcggttcagt gtcaccttca tcccagtaaa aaccatcaac atctgacagc agcaccaaaa
    23881 gatttgcctg cacaatattg gcaacatggg cagacagtgt atcattatcg ccaaatttaa
    23941 tttctttaat tacaactgaa tcattctcat taactatcgg tacaacaccc atttccaaaa
    24001 gggcgtttaa ggtatggcgg gcatttaaaa acctgcgccg atgttttaca tcatcttcgg
    24061 tcagtagtat ctggccaact tcataaccat atttgttgaa gcattcacgg tacacatcca
    24121 taagtatgga ttggcctata gccgccagag cttgcttttc aggtatggtg cacaaattac
    24181 gttttttctt cataaccgct gaaccggcac tgatagcgcc ggaacttaca ataacaatcc
    24241 gatatttttt ctttaataaa aatacaacat catcaaccag acgggtcacc tgcttttttg
    24301 atatacctga ttcatcagta attagcgatg acccaacttt aaccacaatt gtttttacat
    24361 gttttaaata cgcagaacgt ggcatgtaac tattccatta acaaaaaaag tttttctatt
    24421 aatatatcca tattgtatcc tgtcaatgat gatatgggaa gcactgtata tttttctttt
    24481 tcaagcagtg caacacgctt cagtattact gattcattca cactatcgct ttttgttaac
    24541 aggatcatgg atgttctttg tggcaaatcc ttattatacg tggacagttc agcctttaac
    24601 agtgaaagtg tgtacagtgg gtcagcatca attgcttcaa tacagaacag gatgatccgt
    24661 acacgctcaa tatgctgtaa aaacgaaagc cccagaccaa gtcctttatg tgctccctca
    24721 atgattcctg gtatgtctgc tattttaatt acagtttcac cccgctcaat aacccctaaa
    24781 tttgggataa gtgtagtaaa cggatagtca gcaatctttg gctgagcatt ggtgatagct
    24841 ttcagcaatg ttgatttacc tacattggga agccctacca atcctacatc agcaattaat
    24901 ttaaggttta gtaaaattga acgttcttca ccgggcatac caggctgtgc aaaccgtggt
    24961 gcttgatgtg ttgctgtttt aaaaaacgca ttgccttttc ctcccatacc acctctggca
    25021 acacacactg tattaccttc acgaacaaga tcagctaaaa gttctccgga ttctgcatcc
    25081 agtacctgtg tacctgctgg cacgtttata atcaaatcct gcccatcctt gccatgacgg
    25141 ttctggccca taccaaaacc accattctgg gctttatata tcctatcctt aaaaagatgc
    25201 cccaggttat aatatccctg cgtggcctgt attatgacat caccaccttt accaccatca
    25261 ccaccatcag gaccaccctt ggggatatac ttttcccttc tgaacgaaac agcaccggca
    25321 ccacctttgc cagcacgcac tttaatgact atgttatctt taaattttgc cacgtttttg
    25381 cctgcatcac ttacaataat agaatatagg atagctcatt atatttgtcg tacatgaaaa
    25441 tagagtatat tcacacttct catgctgatt ttatcagaaa cgagaaagct tctccagtga
    25501 atcaacatga tttttcctta cttcattcct caaatgacat ttcttgtttc atcatttcaa
    25561 accataatct tctacctgtc atttcgaagc ccgattgcat cgggatggga aatcttttta
    25621 ctaaaataaa caagatttct cgccatttca tatgtggaat cccacccctt tcgctttacg
    25681 aaggagagag gccgggcatg atgtgaacat aactctcaaa ataaaaaaag cttatattgt
    25741 tacaataaca atacaagctt taattattag tatagctata actataataa tatactgtac
    25801 cttatgcctg aacaggatac accgatacct tttttctttc cttatcaagg cgttcaaatt
    25861 ttactatccc gtcagtaaca gcaaatagag tatcatcgct gccacgtttt acattagtgc
    25921 caggatgcag ttttgttcct ctctggcgta ctataattga gccacttttc acaaactgcc
    25981 caccaaaaac cttgactccc aagcgttggg accgggaatc ccgtccgttt cgcgatgaac
    26041 cgccaccttt tttatgtgcc atatattcac ctcattattt cagttattgt cgtaatatat
    26101 tatagttact cttttatgct tacacgtaca aattcaggat attgtttcct tatctcattt
    26161 attccaagga taaaaacgct aactaaactt tcacaaagaa tcttcttatc atcaagaagg
    26221 ctatcgagat tgacagaaaa ttccagtaat ccctcctgct gactgatact atgagctatc
    26281 tcataatatt ctaaagctaa agcaactgat tgcgataatg ccgaaacacc agcacacact
    26341 atatcattgc cttttgatgc ctgacctgca tgccctgtta tcctgacagc aaaaacagaa
    26401 cctgacaggc taaagtggtt gtttgtaacg tctatcccct gtactatgat agtgatcact
    26461 atttaactga catatcaaca atagtaatac tggtatacgg ctgcttgtga cctaatgtcc
    26521 tggtgtaatt tttacgcttc ttaaatttta taccacgaac tttctttcca cgaaccacac
    26581 caccaacttt agctttaacc tctacatttg ataaatacgg ctgaccaact aaaactctat
    26641 cgccatcaac aagcatcatt actttatcga tagctattgt ttcttcatcc tttgcttcaa
    26701 gcaggtcaac agctatagtt ttatcctttt ccactttata ttgctttcca gctatttcaa
    26761 ctattgcgta catatacttc tcccggattt ttgaaattgc attacataat aacaatggga
    26821 ggacatcctc cctgtattgg gcccacaggg attcgaaccc cggacctact gattatgagt
    26881 cagccgctct aacctgctga gctatgggcc cctgtgaaat gacaaaataa taatactagc
    26941 attattatgt caataaattt aaacagtata atactatatt tttataatat tattcacaat
    27001 ctatcgttta atacaatata atataaccaa tcaatttttt ctaaaatttt gttgtataga
    27061 gctttgaagc ctaccaaaaa tatggtggtt gtaaagaatg ataccctttt ttaattcctg
    27121 gcaacatagg gacttgatga tttttcggtt ccaaagtaaa actgtttttc attaatttca
    27181 attgcgtcat tatatgcctc aacaatacca actacatgaa tgagcgctcc aacaacaaat
    27241 aacaatcccg atagtataag ccgggatttt ttttcggttg atgaatctgt tttaatcagt
    27301 gctacataga ggtaatatgc cgaaacagaa tatgcagtag ctgttaacag acaaaaaccc
    27361 gctgctttga ggtcattgcg cagataaaca tgccctcctc caggaactat taatgacaac
    27421 agacatgcca gccgtggatc ccgttgcatg gtgggtgatt cttcacggcg ctcctgtgct
    27481 tttttagaaa attcatcctg atagggtaca tctttatatt ttttacctgc gtcggattct
    27541 attgtttgtg ccatcagagg gtaaccaata aaatttacag aaagtgtaat aactaatagt
    27601 gctgcaatgt acttaaaaca atgtataatt cccttgccct tcttatatac cataaataat
    27661 atcccttttt taatattata caattacata tgttgagata gtcccgaaag atgctttatt
    27721 atatttgcat ataataaaaa agtcaactat tattcttgtt attaagactc ctctgaaaaa
    27781 atgcaagcaa aaatattata aaataattag tttgtgacaa acaataaaaa attaatttac
    27841 aacccataac agggaaatca tagtatcaga actatctata attctattaa ataattttac
    27901 atccattatt ctgccataaa aattgtcata ctatattaaa aagctaaatt aattatatgt
    27961 ataatcgtta cataattaca aaaaaacata aggtataaat aatgattaag gagataataa
    28021 acaagagtaa gtccgagcta attgaattac tctggcagat aaacccggaa atttatgatt
    28081 tattaaaaaa ttcacgtaat ctggatgaag cacgtgaatt tataatggat tattttgacg
    28141 tgcttgatag ggagtttttt aatataaaaa gcaaaagtga ttatcataaa atgcatatta
    28201 taattaaaaa tcaggccaaa gaatgtatta gaattgcaaa aaatattatg cgcactgaaa
    28261 atgaaaaaat atgcaatttt tctgctcttg aagtcctttt taagattgct aaaggggata
    28321 caagtgaact tgaaaagata agtggaggat ttattgttga acttatatat ctatttaaag
    28381 ggataagggg tcagagcgat gtaatacatg ataatgaagt aatcccttcc gatgaccatg
    28441 aattttcaaa aacaagatcc gaacgactcg attactatgc tgaaaatatg tataaaaggc
    28501 tttcacaata taaaacaggc tttgatgatg aaataattga acaacgaaaa aaaatgaaac
    28561 aaaaaatatt aaaatacttt ggcagcgatg aacaggactg gaaaaattat aaatggcatt
    28621 taaaaaatat aatcagaagc aaaaatatac ttaaagatat tatcaaactt gaagaggatg
    28681 aaattgaagg acttgattat gccgaaaaat ataatatacc ttttgaaata acaccttatt
    28741 atctttcatt attcaatgaa aatggaagga gcagatttga tagagcaata agggcccagg
    28801 ttttaccttc aaagacctat tgtgaaaatg tttacaataa taaaaaaaat aacattgata
    28861 tggactttat gggtgaaaaa tcgacttcac ctattgaagg tataacacga agatatcctt
    28921 ttattgtaat attaaaacct gtaaactttt gtcctcaaat ttgcgtttat tgtcagcgca
    28981 attgggaaat agagccaagt acaaatctgc atattaataa aacaaccctt gaaaatgcca
    29041 taaactggat tacaaacaat aaaaatatta tagaggttct tgtaacaggt ggagatcctt
    29101 taataatgac tgacaaatat attgaaaata ttttattgga gctatctcaa ataaaacatg
    29161 tagaaagaat cagaattgga acaagaactc cggtaacgct tccttttaga attaaagatg
    29221 actttgtgaa aatgattaaa aaatatcata aacccggtta tagagaaatt tgcataatga
    29281 cacattttga acatgtagca gaaatcactc ctgaggttat tgaagcagtg caactcatac
    29341 gtgaagcagg tattagcgtt tataatcaac aggtttttac ttattataat agctttaaat
    29401 atgaaacatc agcattgcga agaatgttaa agttaattgg tattgacccg tactacacat
    29461 ttaacacaaa ggggaaaaag gagaccattg actacagggc cccaattgcc aggattgagc
    29521 aggaacgaaa agaagaagca agatttttac caggcgttat cagaacagat gagcctgttt
    29581 tcaatgtacc aaaacttggc aagtcaaatt taagggcatg gcaggatcat gatgttttga
    29641 tgataaaatc aagaggcgaa agggtttacc gctttttgcc atgggaatca aatgttagcc
    29701 ttgttgatga ttatatttac actgatattc caatttataa ttatttaaaa cgtcttcaca
    29761 atgatggtga aaacgttgaa gaatatgaaa ccatttttta ttattattaa atatatatag
    29821 taaaaattta taaattttcc ttaacgcgtt cccatacttc ctttacatcc ataggctcaa
    29881 acttgctctg attaaacctt tcctggcata gattatatat tgttccattg taaggtgctt
    29941 taataccatt ttgttttgcc agttttacaa tatatcctgt tagcgaatca agttcagaca
    30001 tatgcattcc tctttgaatt atatcctgtg acattgagga cttaaccatt ttttttacat
    30061 tcttcaaaaa cagtgggcgc gtaattaatc gaggaagctt tgcagataat ttaattaaaa
    30121 tccatggcgg cattccaccc aatttacatt cattgtaccc ggctttcttt acaattttta
    30181 ctccttcata aagtgtattt gataacaatc gttgaaaagt atcaatatcc gatatctccc
    30241 tataaccatg gcctattaat gttgttaacg catttgttaa attaataacg attttagaat
    30301 gaactgcatc ctgtaaatgt tttgtaacta tggtttcaac accattgcta aaataatcag
    30361 ctattacctg catttcatcc atcaatgaat tatcaggtgt acctaaaatg aaaggcccct
    30421 ttttttgata acctgcaaca ccaggtgtat caatccatgc attatagctt attaccccat
    30481 aaattatttt atcaaaatat tttggcagta tatattgatt atctataccg tttgccattg
    30541 aaattatagt ggctgaattt ttcaaaacat cctttactgc tttggcacac ccttcaaggc
    30601 tataattttt tacacaaaaa attattatat cagcgtcaga tatctcctca attttgtgca
    30661 ttgttttaat tttataattt tcccttttat cagggctatc aatatgataa acagtaatcc
    30721 catttatatc tattacatca gttatttcct ttttatcaaa gcaatataga ttatcatgaa
    30781 aaggatataa ccatgcagca agtgttgctc caattgcacc tgttccaaaa attacaattt
    30841 ttttatcctt taaatttaat tcattcattg ttttcctcct taattaatca ttaagttttt
    30901 ttaaccatga tggtgggttt tcatcaggca aatcaaagca actattcata tgctctgcat
    30961 atctgaactg gaatggatga ggttcaatat caagataatt caaatattca gcctcttttg
    31021 aattccctat ttcaataaaa ttcccttcaa catctttttt aaaaagatgt gaaaaatatg
    31081 tattcacatt gaatgtagct ttaaaacatg ttttactcct gatgattttc ccattatgtc
    31141 tgctatataa atagccagtt tcatcaaatt cctgcttttt acccttttta ggaaccttta
    31201 cagtcaaata ttctttacca tcgctatctt ttgcaattgt tgtgcacatt tcatcagtat
    31261 gtatcatttc aatttcatgc gtaacctttg gcagtgacca tattttatgc cctctaaggg
    31321 tattttcata tgatgttaca ggcatacctg caataaaata accaaaatgc ttaaatgttt
    31381 tcaaaaccat tggtaaaaca ggaaccttta ttttttgatt aacaagtatt gggatagcaa
    31441 tagcaatttc attatatgga ttaatattca taacattttt atattcatag catgaaaaag
    31501 caactatagc tctgcctttt aacatactaa ccggataaac ctttttcccc aatgtttcct
    31561 gtacaatatc ctttgcccta ttatatgaac aaaaacatat tgccatggca catgtaacat
    31621 ctccatagaa tgtaggaaat ttataatttt tttgtatttt atcagttagc tttattggcc
    31681 aatcagcatg cctcaatgta aaacgtttaa aaaaagggtc tttaaactta tcttcattat
    31741 tcagcattgt ttgtgggata tcaaaggatg gcttcataat aaaactccaa ttataaaatt
    31801 ttattagtag atatatacca ttatatgacc gctggtcatt ttaatcaagc taaatttaaa
    31861 atttttttta atttatctta cttattatat ttatttcatt cagagcgagg tattaaaata
    31921 tcctgtttgc aatcaaaaaa tcttttaagt aagtctttta gcatatttgt tgcttcatct
    31981 tttgatttta tttgtttttc aattgaatat ttatggacta actcaagaat tgaaaaaaag
    32041 ataatttttg catattcatc atattgagtg attctataat catatttttt atttatatac
    32101 ttaataatga aattgctaaa aaacattata gtgttttcgg tgtattttaa ttttttattc
    32161 gataccatat taattcgtaa ttccgataaa aataatttat ttttatcaaa aaaatcaata
    32221 attgactcag taaaataatt aatttccgcc ctgtattgaa ttttacttat aacattgttt
    32281 ataaattcat taagatattc ttcaaatatt gaatttatca aatcatcctt gctttgaaaa
    32341 taattataaa atgttccttt agcaatatta cattccttaa ctatatcatt taccgaagtt
    32401 tttaatgtgc catatttttg gaatagttta attgcttttt cttgtatatc agttcggcta
    32461 gttatgcttc gtttttttgg aaatttgctc ataattttat attttcatac aggaagaaca
    32521 aaatgttaag gacacaaacc acatactcag agagtattgc aagcacaaat ttttactcaa
    32581 tggatttgaa aaatagtaaa gatatatgaa gatcaactaa atgcaatggc ttatcgagta
    32641 ttagtagcat atagtgatgg agcatcagga gagtttattg gaggcaaggt aacaacacat
    32701 gttctttgag caagcgatga tccattcatc aagaaatatt gtaatagata tattaaacgt
    32761 tacacagcag gtatactgtt attaaccgat attcacataa ttcgtaacag agcatatagt
    32821 ttttaagcct gagatactga aatatatacg agagtagata agcaagatag cagtttggta
    32881 tgtttagtat ctatcaatgg actatcaaca cggaagacaa gtgctgttta gagcaagcac
    32941 ataaccggga gtattaaaag gcaccgtaag ttgagcagta cggcaattga tgcaagagaa
    33001 cataaaataa ataggccaat caccttgaaa tttatataca ttcaggtaga tactgtactt
    33061 tattactcct ttttttagta aaaaactctg tttatgtaat ggaataactt gttatttatc
    33121 agaaagcaga actgttgtcc tgtttcgacc ctgctgcttg gaaacatata atgctttatc
    33181 agctcgatca ataatattct ttgttgatat atcgatagca ggattgaatt gtgttatacc
    33241 cagtgaaaca gtcactccta acttttttct tttaaaaaga acatatgact tttgaactga
    33301 tatacgaaca cgctcagcta ccttaaacgc ttcattaata tcagtatgag gaagtataac
    33361 agaaaattct tcaccaccat agcgtgcagc aatatcactt tgccttacgg aactggaaat
    33421 aacagaagca acatttttca aaaccatatc accacagcta tgaccatatg tatcattgaa
    33481 atttttaaaa tggtcaatat ccatcattat gagtgacaga ggcaaactgc tttttaacga
    33541 tttttcacgt tcttctatta atgctgtctg aaaataatgg tgaattttga gctttgtcat
    33601 catatcggtt gttgccagct catacagata agcattttga atggcaattc ctgcaaggga
    33661 agctatgttt aacatatact ccttatcaga ttcgctgaag cctttctggt ttatccggtc
    33721 agcaagaaca attattccat tgagagtacc cttgcccttt aacgggacta tcaatgatgg
    33781 gtccaatcgt atgagatttt ccagtgaagg ctcatcaggc agaagctctt ttaattcatt
    33841 taatgtataa caacggaaat tggcatcaag aaactggatg agtttggaat tagtaggaat
    33901 taagtattca tttttatggt ctatttcaaa gcctttgtaa ttcctgtgta atacaaattc
    33961 ttgccggtca atacttctgt gtgtaaatat accagccttt agaagttgca tctgacccat
    34021 acaggttaag agaattgaat caataagaac attataatct aacgttgaat ttaatccctt
    34081 gcttatttca atcaactgct ttaaatcata gattttccgc tcatactgct ctatttcaat
    34141 ggggaatttt atatcttcat catataatga catgtcatcg ttcatgtgtt tcaccattta
    34201 gtcacacaat gtaatagcat tgcgtaaatc tatcctccca tcatatatgg ccttaccaac
    34261 aatacaacct tttaatccat gattttcata tttttttaac ttttccaaat cttcaatagc
    34321 tgaaatacca ccagaagcaa taagagctat gccaggaatt tcagataaaa ttttttctat
    34381 ggacgtataa tttggcccct gcagcatccc atcagttgct atatctgtat aaatgatctc
    34441 agagatgcca atactttgaa gctcctttat acatttgatt gcatccatgt caataatttt
    34501 tttccagcca tgagtagcta ttttcaaatc tttggcatca acaccagcaa taatatattc
    34561 cttaaatcgc tccgctatct ccctgaaggt atcctccatt aacaccgttc ccataataat
    34621 cctttcaatt ccttcatcca gatacatctt tattgcagtt tcagtcctga taccacctcc
    34681 tatttcaatg gggattgata ctgcttttgc tattcttttt actatatgca tattgacagg
    34741 ctgcccttca aaagccccat caagatcaac tacatgaatt aattttgctc ctgcttcttc
    34801 aaactgcttt gcaatacttt cgggatcata tgaatacact gtttcctgta aagggtcccc
    34861 ctgcaccagt cgaacacatc gaccttcttt taaatcaata gctggtatta cgagcatgcc
    34921 aacctcacaa aattttctat catgcgcagt ccatatttat ggctcttttc aggatgaaat
    34981 tgtgttgcta tgcaattacc tttcccaaca atactggcaa attccactga atactgtgtc
    35041 tggcctatta tccatgagct atcaagcact acaggataat atgtgtgaat aaaataaaag
    35101 tagctatatt gcggaatacc gtcaacaacc gggtgatgct gtttgaaaac aacctgattc
    35161 catcccatat gcggtacttt taaatcatta gtaaatcgta ccacattacc aggtattatg
    35221 tcaagtccct tatggtggcc aaattcttca ctggtactga ataaaagctg ataccccagg
    35281 catattccaa aaaaatatcc tccaccccgt atgaaatcaa caagcggctc aacccaccct
    35341 agcgtagtta aattttccat tgcatgccca aatgcaccat caccaggcat tatcagtgct
    35401 ttggaagtag ctatactgcc agggtcagag cttatttgga catcgctggt atatttttcc
    35461 actgccttaa caactgagcg caggtttccc ataccataat caataacggt aatcatatga
    35521 ctccttttgt ggaaggaatg ccagtgctat cgcttattgt ataggcccgg tacagtgcta
    35581 tgcctgcagc tttgaatatt gactcgtgaa tatggtgcct gttatcgcca tataattgtt
    35641 gaatatgcat ggtaatttgt gcattgtgtg caaatgcttt gaaaaactcc agtgtcaact
    35701 cctcgctata tgtttgtatc aaaccagtta acttgtcacc ccggtataca aaatatcctc
    35761 gccccgatat gtcgacaatt acactgctgc atgcttcatc cataggtact atcgcctggc
    35821 caaaccgtac aatgccctgt ttatccccaa gggctttctg gaatgccttc ccaaggcaaa
    35881 ttcctgtatc ttcaattgta tgatgagcat caacatctaa atcacctttt gcattcaact
    35941 gaatactcat tcttccgtga gctgcaaaaa gtgtcagcat atggttaaaa aaaccaatgc
    36001 cggtttgtat ggtgcctggc tctatgctat caagtaaaag ttttacggtt atatcggttt
    36061 ctttggtttt acgctcaata tgtgcagacc gttccattga tatattctcc tgtatttaaa
    36121 actcatatcc taaactgaaa acaatttctt tatgtccaaa ccctgaataa taatatggcc
    36181 aggcatagtc aagctttaaa ataactatgg gatatagcgt aagcctaaat ccaaaaccca
    36241 tatccatttt aaaatcctga aattcccccg tatctttatt cataaatgta tacgtattat
    36301 cccacgccga accagcatca acaaataaaa cgccaccaat attaccaatt tttaaaaaca
    36361 atggccaacc cattcgtatt gattcaatga acgtaaacct gaattcagcg ttgaacagga
    36421 aaacattggt accaccatat tctataaacg gatgaccacg cagcgtatta aatccaccaa
    36481 tatagtactt gaaatattct ttcttttcac ccattatttt cccgccaatt cctctgaatg
    36541 caagtacata ctgtttgtcc aaaaacaaat agtggcgcac atcaatatca atactgctga
    36601 aagaataatc ctggcctgtc agatttacaa cctgggtaaa cattatctgg ccacgtgtac
    36661 cccgtaaggg agccagaaaa ccccataata cattatcgta gttatacgac accgaaactg
    36721 aatttaaatt ggcaaaaaca tcggggcgct gatctaagta gctgtaatcg cgttcatagc
    36781 ggcttgacga aacacgcgtg ctgaaccggg aaaacttggt aaatgggtag cttgcaatgg
    36841 catatacacc ataatgatcc attgacagag tggaccagta ggcattgtga atcaggtcat
    36901 ttatacttgc cagagtaaat atgccaaaag gatttttttg cctgaaaaaa cctaacgaat
    36961 aatcccatcg atagcgcaga taaaggtact gcacatccca gtttaaataa ttagcactgc
    37021 tgccatagcg aagataatcc gtggttacca ctatgcggtt gtcgcctaaa taatcgctga
    37081 aactcatttg agcaaaacca gcaaaaccat atcctacagt gccaccaagc ccaaaaaaca
    37141 cccagtcagg tgaaacggtc gttgtgtaat tgtcccatat agcctgtgat aatgggaaat
    37201 agctttgtgg caacatgact ggtatatgct cagtattaaa agggggggcg atagttactg
    37261 gtttaatgtc ttcttttatg aaaatatcat acccgccatt ctgataggat acaaatgcaa
    37321 ttttgtttgc atcaggaaat atgcgcggat aaaatatacc ggttgatgca ttggttatct
    37381 ttgtttcgct atatttggat atatcatact gataaacatt gtaaatacct tccctgtttg
    37441 aaatatatac aatactattt ccatcctttg caatatcaac ctgtaaattt tttgattgtg
    37501 gttgtacaat aacttctcgg gcatttgttg atagtgtatg tttaatgatg gcgtattgct
    37561 ggctggtata atcccctgcc gggtaatttg atgaataaaa gatgacagta ttattggcag
    37621 ctaattttgg ataccgctca ctgtatacat cttgcgtgac ctgttttaac gatttatcgt
    37681 gtaatgaata gatatacaca tcactctgta tatttgtttg cccaatgaaa catataaact
    37741 ttccatcagg tgataacgca gggtccttta ttgatctgaa tggcaataca atttcattca
    37801 aaatttttcc attactggca tcaataataa atataacatc cctgtttttt gactgagata
    37861 caaacacaat acctttacca tcaggcgtcc atgacagata gttatcaagc aaatgcatcc
    37921 cttcaaattc agatgtttca ttgcccttca gtatgacacg cgctttttct tcagcttcct
    37981 tttcaccaat agttattatg gaaatggagg aatagatatc cctgttatca atataggcaa
    38041 ttttttttcc atcgggtgag atagctggac aggtattaaa tattgaacgc gttttctggt
    38101 ggttggttat tcgctggcct tcttcctcat caaaatttct tcctgctatt acgggataga
    38161 agcgcttttt ataaaaatgt ttaaattcag tatcaatttc ttcaaggttt ttctttgtgt
    38221 gcactttcaa tgcttcttca aagttatcca gatcccgtat atctcgtaaa aattcaccaa
    38281 ttttaccttt tccatatact tgctcaaagt aataaaaaaa agcctgtcca accttatagt
    38341 aatagtacat attgccagtt cgatacgttg tgaaatccat taaactaatg tactgatcat
    38401 ttaaaaagaa atcacgcatg gtcatgtctg cagtttcatc aaagccaatt gataaatact
    38461 ctgacatgcc ttcagtaaac catagcggta cagaacccat gctcatcatt gattgcattt
    38521 taccggaact atcgcgcata agaatgttaa actgaaatgc atggaccagt tcatgaacaa
    38581 ggacatgctg aaattcatca tacgaaccgt tgaaaggcac aacaactcta tttttaaaag
    38641 cttcagtaaa tcctcctgta ccttcaccaa tgatttgaag aagaatatta ttttcctgaa
    38701 aatcaatatg tgagggaaaa acaatgattg gcaccggatc attgagttca tgttgcaatg
    38761 catcagctat gtggacataa gccttttcag ctataacagc ggtctttgta gcaagctgct
    38821 ccatcccata gggataatac acattacaat ggattgtttt taatatatgc catttaaacg
    38881 tttcaatggt aaccttgttt ttcccaaaag gataaataag taatggggcg gctacacata
    38941 ccagaatagt gacaattata tgtatacttt ttttgctcat gagaataaga taaaaatctc
    39001 cactaaaaaa tagaatgtaa tagataatac atatacatta gcaagtaaaa tttttgttgc
    39061 caacatatat tctttgtgta tattagatta taagcctgcg gtgttcgtgt atggtatgtc
    39121 atattgtagt tccaacacta ctgagcatgg aatctctaat ctgcaggttg atatcatgct
    39181 tctttacgaa gtagatagat acctgtatgc gggaatccac ctttaccaag ggagctcaaa
    39241 tattcatacc actacggcac acgcgggtta ctataaaaca taggcaaaaa tatggaaata
    39301 attacagtat ttggagttcg ctggcgtgtt gctcctttat gggaagttgg cttaacggta
    39361 ttggtaatcc ttttagtaat agctttcttt gcattgctaa aatataatca atatctcaaa
    39421 cttaaaaaat ccagcttaga gcaaattttt atctataaag caaggcagcg tgggcttacc
    39481 ggttaccagg ttcgcatact tcagcatatt gttgcactat ccaaactgag ctcgcctgaa
    39541 attatattta tcaataaagc tttatttgaa accggtttag ttaagtttct gcaatatatg
    39601 gccaaactaa aagaacctcc agacgattta aaagatatct tcaaggatat tgttattacg
    39661 tatgaacgaa tttataaccc tgtagaatat cagcaaccac ttgattcaat tgaggatata
    39721 tctgcagata cactggtttg tggatacagc cagactggta gagcatttat agcaaaaatc
    39781 atgctaattg cagaagatac aatccattgc aggtatttca gaaagccagg gcaacttccc
    39841 gatgaaataa taactgaacc tgtatcaata ttctttttta gaaatggtga tgctgaatat
    39901 tcctttgaat ctgagattac agcaattcaa gaaaacacaa tactattaaa aaaacctgat
    39961 gcaataaaac ggggaaaaga ggttcaccac ccatatatac agacacttat tccatgcact
    40021 attcaaaaga aaaaaaaagt agataaagaa gaggaagaag caatcataca acatgatgac
    40081 aatgagccag tgaatggcac tattgaacgg cttaatgaat ttgaagcagt tgtgcgagta
    40141 gaaaaaacat tacagtacca aaatgaatat accctgtttt ttactctgga aaattttaaa
    40201 attgagatag tcagcaaaat tttagcttct cgcactataa caaaagaaaa gattatctat
    40261 tatacattta aacttattga gatgtctgaa gctgcacaaa atgttatatc aaaatatgta
    40321 aaagagcggc tgtaacaaac cgctctttta ttatttatgt ataaaactca cggtctgaat
    40381 caacagtttc accttcaata cgggcaaact cctttaatag ctccttctgg cgcggactga
    40441 gtttctttgg tactttaatg ttaatcttta ccagctgatc tcctttgcca tacgatccta
    40501 aatacggcat gccattaccc ttaagtctga aaatctggcc attctcagtg cctgcaggta
    40561 ttttcatctt ggcttttttt ccatagattg ttggcacttc tatctcacca cccaaacagg
    40621 caacagcaaa agaaacatcc acaatgctta tcaggtcatt accatgacgt tcaaacattg
    40681 gatgtttttg aatatgaacc acaacataca gatctccagc aggccctccg cctggtccct
    40741 gctcaccttc acctgcaatc ttgaggcggg aacctgattc aacgccagcc ggtattttta
    40801 ctgtaatctt gcgatgtttt tcctcaagac ctgttccttt gcataccctg catggcgaag
    40861 ttataatctt gccttcaccc ctgcatttat aacaggtctg agtaattgag aaaaacccct
    40921 gtgtctggcg tatctgtcct gtaccattac atacgggaca tactgttggt tttgttcccg
    40981 acgctgaacc tgttccacca catgcactgc aaacttcatg acgcggaatt tctatctgaa
    41041 cttctttacc taacgctgca tcttcaagcg taattgaaag gtcatattga atatccctac
    41101 cacgtctgac acgtgttgaa cgggtcctgg taccaaaacc agcaccaaaa aaactttcaa
    41161 atatatcccc taaatcaaaa tcaccaaaga tatccgaaaa atctgtatat gcccctctgc
    41221 caaaaccttc aaatccagca acaccttcat ggccaaattt atcatactgc gcacgccttt
    41281 tggggtcacg caatacttca tatgcctcag tggcttcttt gaatttttct tctgcttctt
    41341 tgcttccttt attgcgatca gggtgatatt ttaaagcaag ctttcggtaa gcttgcttta
    41401 tttcatcttc agatgcattc cgaggaacac ccaacacttc ataataatct ctctttgtcg
    41461 tagccaaggt catcacccct tatttaaaaa gattacaaaa attatacgac aatggcaagc
    41521 attgtcgtat aattgtttat aatttattat tttttcttat catcatccac tacttcataa
    41581 tctgcatcat atactttttc gccactctgt gcgctctcct gctcacctgt ttcaggctgt
    41641 tgtgatttag catactgctc agcaccctgc ttgtataatc gttctgcaaa ggaatgcgat
    41701 gcacgttcca attttgcttt tgcatcacgg atgacattga tatccgttga ttccattgct
    41761 ttccgcagat tttcaatttc tgcctcaatg gatgcttttt cactggcttc tatcttgcct
    41821 tcattttcac gaataagttt ttcaagtgaa tagatgagcg catcggcttc attgcgcgct
    41881 tcaacaagct gtcgtgcctt acggtcctct tctgcatgca gttctgcatc cttcaccatc
    41941 ttcttgattt cttcttcgct cagaccactg gaagcttcta tccggatctt ctgttctttg
    42001 ccagtaccta aatcctttgc agatacgtgc aatataccat tagcatcaat atcaaacgtt
    42061 acttcaatct gcggcacacc cctgggtgca ggcggtatac ctatcaaatc aaacctgcca
    42121 atggttctgt tttgtgatgc cagttcacgt tcaccctgca acacatgtat tgacactgct
    42181 gtctggttat cagcagcagt agagaatatc tggcttttcc gtgttggtat ggttgtgttg
    42241 cgttcaataa gctttgtcat cactccacct aatgtttcaa tacccagtga caacggtata
    42301 acatcaagca gcaacacatc atgaacttca ccagccaaca cacctccctg aatagcggca
    42361 ccaactgcaa ccacttcgtc aggatttaca cctttatgag gctccttgcc aaataccctg
    42421 cgtacaagtt cctgaacagc agggattcgt gttgaaccac caactaaaat aacttcatca
    42481 atatcaccag gtgtaagtcc tgcatcttcc agagctttaa tacatggttc tttggttgat
    42541 tcaatcaggt cttctaccaa cccttcaaat tttgaccggg ttaatgtcat gatcaggtgt
    42601 ttggggccac tctggtctgc agtaataaat ggtatattga tctctgtctg caatttattg
    42661 gaaagctcaa tctttgcctt ttcagccgct tcttttaacc gctgtaaggc catcttatca
    42721 gtacttaaat caattcctgt ttgctttttg aactctgcaa tcagccattc cataatgcgc
    42781 tgatcaaagt catcaccacc taaatgggta ttaccattgg ttgattttac ttcaaacact
    42841 ccatcaccca attccagtat ggaaatgtca aacgtaccac cacctaaatc gtatacagct
    42901 atcttgtgat tacgctcctt cttatcaagg ccatatgcca gtgccgctgc agttggttca
    42961 tttataattc gctcaactgt caatcctgca atacggccag catcctttgt tgcctgcctt
    43021 tgtgcatcat taaagtatgc tggtaccgta ataacagcgc gtgtaacctt ttcgcccagg
    43081 taatcttcag cactctgttt catcttctgt aaaattcgtg ccgatatctc ctgaggagta
    43141 aaatccccgg caatagtttt aactttaacg ccattttttc catcatccat aacagtatat
    43201 gggaccattt ttatttcact tgaaacctcg ctgtacgaac gtcccataaa ccgcttgatt
    43261 gaccggatgg tgttctcagg gttggttatc atctggtttt tggcaacctg accaaccaaa
    43321 cgctcacctt tatcggtaaa tgcaacaacc gatggtgttg tccgttgccc ttcggaatta
    43381 ggtataacca ctggttcacc accaaccata actgcaacac atgagtttgt ggttccaagg
    43441 tcaattccta ttatcttact catagtcatt ctcctttttt atgaatatat tctaatgata
    43501 gtttgtatca ttgtatcatg cacattaatg cggtgacgct gtcgtatttt cctcagtttg
    43561 ttcactggta gtatcaggct tttttgccgg gcgcgtcacc ttaacccgtg cagccctgat
    43621 cacatattta tctaacttaa agcctttttg atatactttg gtaatagtat ccttttcaac
    43681 atcaggactt tcatcaattt caatagcttc attatactct ggatcaaacg gctggttata
    43741 gctttcaatc tcttcaacac catagcgttt cagcatatcc tctatctgcc gtgatataat
    43801 ctttacaccc tcaacaaacg atgtatgtac ctcagctaaa ttttcaacat tctggacatt
    43861 actggcagct tcaattgctc tgattaaatc atcgttgatg gtaataacat caagtgccat
    43921 atcctttatg gctaacttcc tgtcatcctc ctgcatacgg atgacacgtt ttttataatt
    43981 ttcaaaatca gcctgcctgc gcatcagcaa atctttcagt gcagtcaatt cttcattctt
    44041 cttattcaat tcttcctgta acttctgcaa tgcatcctcc ttctgttgca attgatcctg
    44101 tgcggcttgt ggctcttctt taccagatgg ctgattcgtt gagctatcgc ccacaggctc
    44161 ctgtgcattc atgtgttcat gtaattcttt attatggtca tgtatttctt tgctcattgc
    44221 attatgctgt tgctcattat tgtgatgctt tttatcctta ccgctcatac atccccctgc
    44281 attcaatggt aatcaaacac tgtgttttga attatttggt catctgtgtt aaaaagtttg
    44341 aaaccacttt gcccgtataa tccacaagcg ggacaacctt ttcataatcc attcgtgttg
    44401 gaccaataat gccaacaacc ccaacagggc tattgccaat tttatatgat gaagcaataa
    44461 tgctgcaaca tgggatagct atttccttta tttcttcacc tattaatgta aatacgcctt
    44521 ctttctggcg caactcttcc ataatcttac gcagagtatc cttctgctca atgaaccgca
    44581 acacctctcg caatgtttcg gtttcaacca tatcaggtat gtgtaaaaga ttttcaatac
    44641 catcaatata gatttccggc tcctgctcat ctttcagtgc cagttcagtt atatccagcg
    44701 ctatatcacg cacctgtggt tcaataccgg tcacagtgcg caattgatta atgagacttt
    44761 gcttaatttc aaccaacgaa taacctgaaa gctccgcagt aaggaagcgg gagtatttat
    44821 acagatcatc ctgattgact ggcgatgaaa gccgtacctt tttatggata acaatgcctg
    44881 tcctggttac gataataaac aacacctcat tggctgtaag cgatataatt tcaatatgct
    44941 tgacaacagt aaaatcgggc tttggcataa gcgcaatgcc cgtatatttt gatatgagcg
    45001 atagcatcct gacaatagac atgaatatct tgtccagttc aatctcacgg gtcaacagtt
    45061 cctctcttac ttccacgccg ttttttgcca ctgtttcata ggtatccaga agtgaatcga
    45121 cataaaaccg gtagccctta tcggtaggta tacgccctgc cgatgtatgt ggctgcgata
    45181 ggtatcccat ctgctccaga tcaaacatga tgttacgcat ggttgccggt gaaagtgaaa
    45241 acgaatactt ctgcacaaat gaccgcgaac ctaccggttt acccgtagat atatactcat
    45301 atacgattgc ttttaaaacc tgtgcttctc gttcatttaa ttgcttcagt atcattgcac
    45361 caccttcatt tgcaattatt agcactctac ctatatgagt gctaataatg tactaaaaaa
    45421 attagcactc gtcaagtcat tttgcttaaa aatgtttaaa tatctggatt ttttagtatt
    45481 tttggtttac tttttctttt ttttctcttc ttcctctttt tcatcagctt caagccgcaa
    45541 ccggagcgcc tcaatggtat ttttaagctt ttcagcttct tctgattcgc caaacagtat
    45601 gttaatcctg cgcaaagctg ttaaaagctc aatagatcgc agaatatcct ctgaggattt
    45661 tgttgatatt tcgtatttat tccggtactg ctttgctgcc tcagacaggc tctgtttaca
    45721 aagcttcaac atctcctggc gatcatagta aaattcttta cggggatccc ggtatttctg
    45781 caaatcactg aaattgatga gatttttggt aatgacagct accctgccct gtaattcaac
    45841 aaaggaccat ttccatttag agctttcgcc taaagcttcc ataatctttt gcagtaaggt
    45901 atgtagttct ttaatcaaat taagaatctg tgaaggattt aatttttcta tcgtcagtaa
    45961 atattcttca ttttctttta acgggcggtc aatgtccttg ccaactatct cttcaaataa
    46021 ttgcaacact ctgtaaaact ctttacgtgc atcgtttaaa aaacgttcgt ttttaatctt
    46081 cagcatatcc agcgatgctt tgttcatatt gagtaatgta tatatgtatt ttgtatacag
    46141 taaaataagc tcaatattaa cataaccctt aatattgggc atttttttct ttttctgctc
    46201 agtttccttt atctgtttgg tcaattcatc taattttgat ttataccctt taataaagtc
    46261 attgtacgct gctttctcgg cttttgtgat tggcattgat ttttccctct atgcataagt
    46321 gtcgatagtt gtttcttttg ccttctcagg ttctttttta acttcagttt ggacttttgg
    46381 ctcttcctgt tgctgattat tcaccgaaaa ccccgcagga ataacatctg cacctgaaat
    46441 tcgcattatt tccatagtat ccctcctcca ttaaggatgt catgggtctg tcccaaaaga
    46501 ggtcgttgag taaattttgc cacaaaatct ggcaaaatta atcaatgagc gttggaaaat
    46561 ttgtattgaa acgacttttg ggacacaccc atcttgcttt taatactcta taatctttaa
    46621 tatagccaca gttttagata tccacatgat tcgtcaatag atagctataa agcctcttta
    46681 actcatttac tctgcgttcc ctgcgtgcaa cacctcttgt atactctgtc actaataatg
    46741 ctttgataaa tatctacatt atacttatat aaaatatcaa gcaaaaaata aagttgcaat
    46801 aaatctaatg ttaattgaca ctatcaatcc atatctcaca aatatgcaaa aatcattgga
    46861 ggaatccctt gccaaaacga agtgatatta aaaaagttat gataataggt tcaggcccaa
    46921 taattattgg tcaagcatgt gaatttgatt attcaggaac gcaggcatgt aaggcattaa
    46981 aaaaattagg ttatgagata gttctggtaa attcaaatcc tgccacaata atgaccgacc
    47041 ctggaatggc agatgtcacc tatattgaac ctttaaccgt tgaatccctt gcaaaaatta
    47101 ttgaaaaaga aaaacctgac gcattgcttc ctaacttagg tggtcaaaca gggcttaacc
    47161 tttcggctga actgtcccga caggggatat tgcaaaaata cggtgttacc ataataggcg
    47221 ttgaagttga tgctatagag cgtggagaag accgtattgc atttaaaaat accatgaatg
    47281 cactgggcat acccatgcct caaagtgaac cagcctatag tattgacgaa gcaaaaagaa
    47341 ttgctgacaa attgggcttt cctctggtta ttcgcccagc ctatacgctg ggaggtaccg
    47401 gtggtggtca tgtatataac atggaagagc ttgaaaccat tgtgtcgcga ggactatcgg
    47461 ccagtttgat tggccaggtt cttgttgaag aatcagtcat tggctgggaa gaactggaac
    47521 ttgaagtagt gcgtgatgca aagaatcaga tgattactgt atgctttatt gaaaatgttg
    47581 acgcaatggg catccatacg ggtgattcct tctgtactgc accaatgcta accataagcc
    47641 aggaaacaca gaagaagctt caggaatatt catacaaaat tgttgaagca ataaaagtta
    47701 taggcggcac caacatccag tttgctcacg atccaaaaac cggaaggatt gtggtcattg
    47761 agattaatcc tcgcacgtca cgttcttcag cactggcctc aaaggcaaca ggattcccta
    47821 tagcatttgt atcatcactt cttgcagcag gtttaaccct tgatgaaata ccctactggc
    47881 gtgatggcac tcttgataaa tatacgcctt caggagatta tgtagtagtc aaatttgcac
    47941 ggtggggttt tgaaaaattc aagggagtag aggataagct tggcacacag atgcgttctg
    48001 taggcgaagt tatgagtata gggaaaaact acaaggaagc attgcaaaaa gcaatcaggt
    48061 cccttgaaat tggccgatac ggacttggtt ttgccaaaaa ttacaacaat cttgaccttg
    48121 atgaactgat ccgtcttgtc agcacaccaa ccagcgaacg ccagtttata atgtacgaag
    48181 ccctgagaaa aggggcggat gtagatatac tgcatgaacg cacccacata aaaaaatatt
    48241 tcatagagca gatgaaagaa ctggttgaac tggaaaacca gatgcttgcc tacaaaggaa
    48301 aatcactgcc tgatacattg ctcacgacag ccaaacagtg gggatttgcc gacaggtatc
    48361 tttcaaaaat actggacata cctgaagatt caatccgaag gcagagggtt aaattgggtg
    48421 tagtagaacg ctgggaacca gtacccgtaa gtggagtgga aaatgctgca tattactatt
    48481 caacatacaa tgcaccggat aaggttgcag tctctaaccg taaaaaaatt atggtgttag
    48541 gcggtggccc caacagaatt ggccagggta ttgaatttga ttactgctgt gtccatgcag
    48601 catttgctat caaagaagct ggttatgaag ctattattgt aaactgtaat ccggaaacag
    48661 tttctactga ttatgacact gctgacaaac tatactttga acctctaaca gtagaagatg
    48721 tgctgagtat ctatgaaaaa gaaaaaccgg aaggggtcat tgttcaattt ggtggtcaaa
    48781 ctccattgaa tatagccggt gagctggtta aagctggtgt aaaaattctt ggaacatccg
    48841 ttgactccat tgaccttgcg gaagaccgtg accgtttccg caaagtgatt aaaacattag
    48901 gcatacctca gccagaatca ggtatggctt catcgcttga agaagctatt gctatcgcgc
    48961 aatcaattgg atatcctctt atggtgcgcc cctcctacgt gctgggcgga agagcaatgg
    49021 agatagtata tgatgaacat atgctgaaac agtatgttaa ggctgcagtt gaagtaactc
    49081 ctgaaaggcc aatccttata gacagatttc ttgataatgc aattgaatgt gaagctgatg
    49141 caatttctga tggcaaagat gcttttgttc cggctgttat ggaacatata gagcttgccg
    49201 gtgtccattc aggtgattca gcatgcgcta ttcctccatt caatatcagc aaaaaacacc
    49261 ttgcaacaat agaggaatat accaaaaaaa ttgcccttga actcaatgtt gttggccttt
    49321 tgaacataca gtatgctatt gccaatgata cagtgtatat tctggaagca aatcccagag
    49381 cttcccgtac tgtaccactt gtttcaaaag tgtgtggcat cccaatggct aaaattgcaa
    49441 caaagattat gctgggcgaa aaactcaaaa catttaatct taagaaaatt tctatcaagc
    49501 attttggtgt aaaagaagca gtatttccat ttaacatgtt ccctgaagtt gacccggtac
    49561 tgggccctga aatgcgttct accggtgagg tgctgggtat tgccaataac tttggcgttg
    49621 cctattttaa agcacaggat gcggctggtc aacgattgcc actggaagga aatgttctga
    49681 ttacggttaa ccgcaaagac cgcgaaggag cacttgaagt tgcaaaaaat ttctacaagt
    49741 taggcttttc aatttttgca acagaaggta catcgcagta cctcaatgaa catggtatcc
    49801 catccacaat ggtgttaaaa cttcaggaag gaagacccaa catcattgat aaaattaaaa
    49861 ataaggaaat taatttaatt atcaataccc cgtatggacc atcaagcaaa tgggatgatt
    49921 cgtacattcg taaggctgct atcaggtaca agatacccta tataacaact atcgcagcag
    49981 ctaaagcagc ggtaaacgga attgaaatgc gtaaaaaata tggggatgaa atcccaaaat
    50041 cattacagga gtaccatgct gaaatgaagc tgacataatt attatttaaa tttttctatt
    50101 gacttttttt attgtatcta tctaatgaca aaaaaagata accaaccaac tgggattatt
    50161 gtaaaggcgg tttcaataat caattgcatt ttttttcata atcttcttga ctattatatt
    50221 tagagaatct cattgtaaga aaaaattaat agattgttgc tataaatatg gaatggcatt
    50281 aaaaacctat taatttttca gataaatctt ttaaatgata agagaggcgt accttgacac
    50341 taaaaaatac tgaaattcaa aaagccgaag gcaaattagg ggtgcttatc cccgggcttg
    50401 gtgctgttag caccactttt attgcaggtg taatgcttat tcgtaaagga ttaaagcttc
    50461 ccaaaggctc tgtagcaatg atgggaaagc tcagaatagg taaaggtgat gatgcacagt
    50521 atgtgccaat caaagatttt gttccactgg ctgatttaaa agatatggaa ttaggcggat
    50581 gggacatttt tgaagataat gcatatgaag cagcagttaa agcaggcgta ttacagaaac
    50641 cggaccttga gccaataaag gaagaattat cttcagttaa accatggaaa gcagtttttt
    50701 ttaataatta tgtgaagaag cttcatggaa catgggtaaa aaaagctccc agctactggg
    50761 acttagccca gatggttaaa gaagatattc tcaattttaa gaaagagaaa aatatttccc
    50821 gcatggtaat ggtgtggtgt gccagcaccg agatatacca ggaaatgaag ccaggagtgc
    50881 atgacacaat tgaaaatttt gagaaagctc ttaaagaaag caacaaatac attgctccca
    50941 gcatgatata tgcgtatgca gcacttgatt taggaatacc atttgccaat ggtgctccaa
    51001 acctgtgtaa tgaaatacct gcattgcgtc agttagctga aaagaacaaa gtgccaatgg
    51061 gcggcaggga tttcaagaca ggccagacat tgatgaaaac tattattgcc cctggactta
    51121 aagccagaat gattggtgtt gatggctggt attcaactaa cattttaggt aaccgtgatg
    51181 gcgaagttct tgatgaccct gaatcattca aaactaaaga agtaa
//
LOCUS       DAORYL010000013        45297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_662_length_45297_cov_12.754564, whole genome shotgun sequence.
ACCESSION   DAORYL010000013 DAORYL010000000
VERSION     DAORYL010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 45297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45297
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_662_length_45297_cov_12.754564"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PLV81_04265"
     CDS             <1..277
                     /locus_tag="PLV81_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09350.1"
                     /translation="YIHEHENTIINIIHKYKPDLFVDVGSTDMIKKVMVYRKKGDPVF
                     YFNIHETPILRLGRFASLDDFPKIVKYLNAHYEFKGYFGKARLWVRK"
     gene            262..1824
                     /locus_tag="PLV81_04270"
     CDS             262..1824
                     /locus_tag="PLV81_04270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOM09351.1"
                     /translation="MGKKIINNKFYLFALMFLLILVLRIPALYHTIIDIDEAAFAEFA
                     NKWLGGAIPYVGVYDNKPIVTYLFYYIIFYLFGINNLFAVHVATLFLVFISTVVVYWF
                     IEKIAGMDEAVASSLFFIFLMHTYEPKYIATNTETLYTLPVLLAFVWYYVVVWEKKSI
                     LYAVLSGMCAGIAFLINYKAGVIVAAFGVFSIYVLCKASDKKGELLNQIKILGSIGIG
                     FIIPVCIAIVYFFHKRALNEFITLGFLYNFKYIQSGMVSVPYLKAIARFVLFGICSLP
                     LWFLIVRQLFYEYEKSNTDKNLILLLLIWLFGSIFAVMLGWRAYGHYFIQLALPLSLL
                     AGLSIHSVSALFLKRSYVFLTVLAVLLTVSRINIPLTYHFLGDSNALSDFAYKNVAIR
                     VQAITSPDDTMFVWGSGASAYIYANRRCSSKIIITDYVSGRQFGVSNEDMGENVNKYI
                     QYLRDKFIKEFMQNPPAVFIDTSPSGYYGYDRFPLKKFPDLSEIIKNNYSFDCNIDDM
                     NIYILNKYTKKQ"
     gene            1895..1967
                     /locus_tag="PLV81_04275"
     tRNA            1895..1967
                     /locus_tag="PLV81_04275"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1928..1930,aa:Thr,seq:tgt)
     gene            1974..2055
                     /locus_tag="PLV81_04280"
     tRNA            1974..2055
                     /locus_tag="PLV81_04280"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2008..2010,aa:Tyr,seq:gta)
     gene            complement(2154..2360)
                     /locus_tag="PLV81_04285"
     CDS             complement(2154..2360)
                     /locus_tag="PLV81_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOM09352.1"
                     /translation="MKNTITKDMTFGELVQKYPTAAQVLASYGLHCIGCHIGIYETIE
                     QGSKAHGLTDTQIKEMLEKLNQAV"
     gene            2648..4042
                     /locus_tag="PLV81_04290"
     CDS             2648..4042
                     /locus_tag="PLV81_04290"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOM09353.1"
                     /translation="MKRMIVVGTYVLLFLIVIIFLSIGHYEKVYADNYTNANLSDCLQ
                     IALQNNINIKMAMEDVNAASLNLKITKGSNNIQVNGTVQTVEILKSDAVNSSFNVPGK
                     DTLIGLFVGAQASYKLYDANKSNYERLRELEYNNIALLALKTKNNIIRDVKIAFYQLA
                     FAKKKSELLEKLVKSFEIKYANVEQLFKNGQRTMLDISRAQVDLTNARLNYQRAQNEV
                     AVAKSKLLTLMGIQIEPFDINIADIDIDSTIKEIPYSLDDLLETALQYSFDIKSAEIN
                     QEIARTQIEIEKDRRLPVINIIASLGYENRAIQNGAFTESLSDRDNWKPTMHAGVSAN
                     FPIFTSGVLSSSIDRAAVSFNKALYNTKTVVDTLSGNIKSYYNSLKDIKVQLDISRQL
                     IENARKYLELVQKNYDRGIITQNELQDAELNYINVTIDYIKILTDYYINYNQLISLLC
                     VDENILFSKGVDAK"
     gene            4039..5148
                     /locus_tag="PLV81_04295"
     CDS             4039..5148
                     /locus_tag="PLV81_04295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOM09354.1"
                     /translation="MIKEIVLNNKKKVLIAGVVIIILILIYSLRSGVDDSEYIYDYEK
                     VSIGEISKTISTTGTIDLLDPVSILCQVNGVVQKVYVDFNQHVKQYQLLALIDAGDLQ
                     NDITKMQKRLEQLNIDLKNMENDLKVKKSMYNEHMISASALEMAENEYKKMQLSYSQV
                     LVDYKNLLQMQKNTRIYAPISGIIVSRDINESDPVVRNKRLFLMVRDLKTMQIMVVID
                     ETDIGKVKSDQKIKFTVNAYPDQTFTGAIKQIRFNPVNRGGVITYDAVAICDNSQYLL
                     KPGMTAIVTIEVDKKQNTMRIPNDAFTVIPPFIDKYKLNKDKKYIWKKKRGTIVPVEV
                     QTGLYGDEYTEIKKGDVKPGDTILIRVRKQISIKK"
     gene            5159..5878
                     /locus_tag="PLV81_04300"
     CDS             5159..5878
                     /locus_tag="PLV81_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOM09355.1"
                     /translation="MNIPIINIENLQKIYKISEEVSVHALKGVTLKVKRGEFVAIMGA
                     SGSGKSTFMNILGFLDTPTSGKYILDGIDGTSLNDNQKAEIRNRKIGFVFQGFNLLSR
                     TPAIENVEMPLFYRGGFTHEEMKNRARKLLAMVGLSGREMHHPNELSGGEQQRVAIAR
                     ALINDPAILLADEPTGNLDTKNTTEIMNVFTTLNKEYNITIIMVTHEPDVAEYADRKV
                     VFKDGLIIDDSPIKKLKRSRI"
     gene            5880..7058
                     /locus_tag="PLV81_04305"
     CDS             5880..7058
                     /locus_tag="PLV81_04305"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOM09356.1"
                     /translation="MDFTNLLITALRSLSKNKARSILTSIGIIIGVSSVIVMIGIGRS
                     AQITVEERVGTYGKNAMEIASFHTLLTEADAENIKKKFPQVKYASIIITKSNVQVNYK
                     DRSTISGMYGVENDYFKMKLWPLTSGRYFTDIEIATHEKVAIIGATVMRDVFIYEEPV
                     GNIVIINNVPFKVIGVLSELGESFGGKDFDNVVVIPYGAAQRKIIGNNKIDRIYVSVY
                     SDKQMDEAVEQIKEYLHRAHMVPPDSNEFRITTSKQKLEMAEYISKTLSMLLAGIASI
                     SLFVGGVGIMNIMLVSVSERTREIGIRMAIGAKRRDILSQFLIESVTLSGLGGIVGII
                     LGLFVYYLITYIVEWPYIFSVTSILLSFLFACAVGIFFGYYPAKKAADLKPIEALRYE
                     "
     gene            7216..8106
                     /gene="xerD"
                     /locus_tag="PLV81_04310"
     CDS             7216..8106
                     /gene="xerD"
                     /locus_tag="PLV81_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOM09357.1"
                     /translation="MKDIIAIKRFKRFLQIEKGLSPNSIYSYTYDLKKFSDFLARKNK
                     TILNASQDDVEQFLFFEKNKKRNSARTLARSLAAIRQFYNYMASIENISNPTVKIETP
                     HFQKALPDFLSIEEIDRLFASIREDDLYQLRDKAIFELLYSAGLRISEAVELKVIDVD
                     LKNKFLKVLGKGNKERIVPIGDEAKRLIEKYLNDSRPIILGNRISEYLFISKKGGMLN
                     RKSVWRLLKGYARMVDIRKNITPHTLRHSFATHLLERGADLRAVQELLGHVDISTTQV
                     YTHLARKQLQEIHKRFHPKS"
     gene            8125..9267
                     /locus_tag="PLV81_04315"
     CDS             8125..9267
                     /locus_tag="PLV81_04315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOM09358.1"
                     /translation="MAIAHVFRWLLIIIFVTIAGCVPPPEYMAKTGKRIGFDNDYIKI
                     LLLRDQLKVEVITTARTKVIQPANNTIVYNGDAKRFVLFDNQLTQPLQFESWNNEIKV
                     NGITVRGMIQLHTVVGKIQVVNVLKMNEYLYGVLPSEIMSGWPEEVLKAQAVAARTYA
                     YYHLMKNNSTYFDLDATNNFQVYKGKAVETEATNKAVDATAGIIMTYNNKPILAYFHS
                     TCGGKTTDDKYVWQGEDLPYLTSVVCPYCKNSPNYSWQVELTLNEIYEALVKRYKGVG
                     KIKAITLGRQGTRVTVVKIEHDNGIIRMSGNDFRLMFDAKKIKSLYFEAKQTRTGLIL
                     TGHGWGHGVGMCQWGAKEMAQQGMRFDQILKFYYKGIRIMTYSMQK"
     gene            9601..10959
                     /locus_tag="PLV81_04320"
     CDS             9601..10959
                     /locus_tag="PLV81_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HOM09359.1"
                     /translation="MKKMSISVLFILFGICVLAIAQTQKFGEALHGLYAYKDFQKATY
                     CRQCHVEIYYQWQKSMMAQAFVHPWDEIEYFDLAVSHGRRNPKFKPVADGCNGCHAPI
                     SFMAGDTPPPRPEKKSMANEGVSCDVCHTIVSFNQNEQYNFSFFAKPGRTKYGPRGGK
                     NSPAHDIVKSDLITKPEFCANCHNEKSPWGVWVKATYNEWKEGPYSKEGVVCQTCHMP
                     KAAGKRAKTENKLYDDLKQHTFHGGHFQAKYNGAIDVLIYPDTDFVEPGMKTKISVYL
                     YNQKCGHKVPTGSVEDRLLYLHVEAIDSKGNVYHLKVDKKGFQGEEYTIASREKAYQD
                     FAEMMDVPADFDGIIREDVPVGDRIFRMPYFTENGIMTIAQWNTAKFGVDYRIAPRET
                     KVETYTWTVPDTVAPGPLTIRATLYYQLLVRPVAQFLKVPESESMDRMINTDVVTIDV
                     IY"
     gene            10991..12220
                     /locus_tag="PLV81_04325"
     CDS             10991..12220
                     /locus_tag="PLV81_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09360.1"
                     /translation="MKTCTMYTIIVLLVIGVLLYCEEQSQAMPGFARRYKFTCQTCHA
                     PFPKLKPYGEEFAANGFRLPEGEPSYATIDTGDPSLELTRNFPIGMRLDAYAVADKDD
                     EVSNDLQTPILLKFISGGPIAQNLSYYFYFFFSEKGDVVGIEDAFLYYTDLLGTGINI
                     TAGQFSVSDPIYKNELRLTYESYVPFAAKPNRSLCNLKYDRGIIVDKGFDFGLTLVAE
                     IVNGNGIDPADEHDNFDGDKYKNYFFRASQELFDVVRIGGFGYIGKEKNTADDTVTDV
                     FTNSVSYYGGDLSIILGPFELNTLFLYRKDSNAYFTENKKSIATKGYMAELIFQPEGD
                     RSNYVITALYNKVDSDYRESGVHSLNYESATLSLTYLLARNVKIIGEYTYLLHDYSKG
                     EDYKNHHILKTGVSAAF"
     gene            12275..13312
                     /gene="queA"
                     /locus_tag="PLV81_04330"
     CDS             12275..13312
                     /gene="queA"
                     /locus_tag="PLV81_04330"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOM09361.1"
                     /translation="MEKKLYLLESYDFDLPQELIAQYPADKREESRLLILDRKSKHLV
                     HEKFYNLPNYINANDILIFNNAKVIPARIYCKRSTGATIEVVLTKRLCDNTWEAICNR
                     LKRIKPGEILYPIKNNSVSIVVVEKSEEGITIKFEPNIDYAMLEKIGELPLPPYIQRS
                     PDKNDYERYQTVFARKYGAAAAPTAGLHFTDDLLKQIQDKGATISFLTLYVSWGTFQP
                     VRVDDIRLHTMHTEEYYLPEKTVESIYNAKAKGGRVIAVGTTSLRVLESVYHNGEYKH
                     GYGATNIFIYPPYTVKSVDALITNFHTPGSTLLMLVAAFAGYDCIMQTYREAVKERYR
                     FFSYGDAMFIT"
     gene            13546..14094
                     /locus_tag="PLV81_04335"
     CDS             13546..14094
                     /locus_tag="PLV81_04335"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOM09362.1"
                     /translation="MLLKGCLHTHTTCSDGTLTPQQVAVRYEEKGYDFIAFTDHDYLL
                     KPGYDEIYNNVKSNMIIFPGIEMTVFLKGYIHVNRIFGDNQLLHIFNHLPEYDLDIEQ
                     IMDRLYNLQKMFAIDAMEITSKGFKVKRFNEMTFPHPSIVSDDAHTAIGIGRAWIELD
                     AKRNKDSILKAIKHGDFWNCYC"
     gene            14157..15302
                     /locus_tag="PLV81_04340"
     CDS             14157..15302
                     /locus_tag="PLV81_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOM09363.1"
                     /translation="MQKRVAICAVAQIKNDPNLWYARFQNMLLECFEGIMSQTKVSFD
                     MEKGIRTVITCSDDVWDARTISNNGMTDVVGAHFRGEEKMAQESLNGLGYAMASILSG
                     HDDVVLYMGHMKESQPESRTMCTNLAFDPFYCRPLGMDFVNVNAMQARAYMEKSGVNE
                     KHLAKIVVRSRQKAKQNPFARENEPIKEDDVMKSPLLADPLRQLHYYPTTDWAFGMLL
                     CSEERVKEFTDKPVWITGYASCMDSYFLGDKDLTSNFSLRNATERALKKAGIKDVKKD
                     IQLFELCDHAAYQLPMWAEGIGLAKEGKGGKWIDDGGMDEFNVNMSGGMLNGNPLLLG
                     GAARAIECFYQLRGEAGKRQVKGAKRALAQSTYGAAGQHQAVVIFEV"
     gene            15316..16512
                     /locus_tag="PLV81_04345"
     CDS             15316..16512
                     /locus_tag="PLV81_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOM09364.1"
                     /translation="MAQKKDRNVAIIGIGQTKHSSHREDVNQPEMIHEAVSAALKDAN
                     ISIKDVDCIVHGNMELFEMIHQPDLWHVLGTGAYGKDSIRITTGGTVGTTLACASDNL
                     VASGMYDIVMAIGFEKLQEGHTTGGITNMADPLWFRNLQTGALTGSTAYDVIQEFGED
                     RAKRAAMMYRIIMDKHACLNPNAHRAFGLDFDQLETLMNTSPQLVGELRLIHMCSQSD
                     GACAVIFASEKKAKELSKKPVWIRDHITIHREETFNIFGYHKDYPVLKTHKFAAEQLF
                     KRNDIKNPLEYFDVFEMYDPAAWWGLDWLRDFFFLKGDEHLRLVENKEIMIGGSMPIN
                     PSGGVIATNPIGATALLRVAEAALQVRGDAGAHQIPKEVKHALASGFGGTMWTVLMML
                     EKELSW"
     gene            16522..17703
                     /locus_tag="PLV81_04350"
     CDS             16522..17703
                     /locus_tag="PLV81_04350"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOM09365.1"
                     /translation="MEYWGVKVEDIFDTMNDRFRPEGAKGVTAKFGYDITGEGKWKII
                     VSDGLLEVIKTDDISDCVAVMVCDGETFVGINIGKVDAANAFTSGKVKIEGDFGQFGK
                     TAKMFKKYVPKKAAMSTRDYILDMFGTLVSRFQPQNAPQEAVIVYDIGGADGGVWTAY
                     IKDGKCELKEERAEKFTVKLEVQEAKDWVDVMLGKVDAFSLLSAGKAAIVGETQLALK
                     LGEIFGKYQDPMASGTPEQELIVLKKIISVDQKFSTGPVMGKFLNALKEKKILGNKCP
                     KCGRIQLPAREVCAECRVRVDEWVEVGPKGQVRYMEYVYYASPDPLTGETRETPYGMV
                     NILLDGCSGNDTFAHLIRRDQIDRIKGGKDDVSGTRVRPVWNEHRTGSIFDIKYFEID
                     E"
     gene            17716..>18374
                     /locus_tag="PLV81_04355"
     CDS             17716..>18374
                     /locus_tag="PLV81_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HOM09366.1"
                     /translation="MALDYDRKDCFVVEGKLALPYSYFAGRVGSTFITTLRDKKKIMG
                     VKCPVCNKVYLPPRQTCERDLTDIRDNWVEVQPTGEVVNFTVVRYDDKHLPRKAPFVM
                     ALIKLDGADTPIVHILDGIAPEEVKIGMKVEAVFSSSPTNTILDISHFAPKKPEKAFV
                     SERKPAGAKGEEPVISEEEKLLKERRKAMSKKVIITAALAGAATMKNQTPSVPYTPQE
                     FA"
     assembly_gap    18375..18474
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18475..18996
                     /locus_tag="PLV81_04360"
     CDS             <18475..18996
                     /locus_tag="PLV81_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOM09367.1"
                     /translation="MNFSIIDRKTGKIFIDFVFENTFTMLQDFGKAMEENGVKPEVEV
                     YDLGGLDNWLLISKQGFFTKPYNFNFVWGVAGGMAFRPDMFMVLKNALPEDANFTTCG
                     VGLEQFPAITMSCLVGGHMRVGLEDNIRIPTGELAKGSYEQVEWAVRIAESLGREPAT
                     PDEAREIMGLKKR"
     gene            19090..19869
                     /locus_tag="PLV81_04365"
     CDS             19090..19869
                     /locus_tag="PLV81_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOM09368.1"
                     /translation="MGNKCYYQMEKENVAVVYFDNPPLNALDTETMEQFENIMSQLVQ
                     DTSVHAIVLTGKGSTFVVGADVNAVMEVDTYEKGVEITSRAQAIVSLCELSPKPVIAL
                     INGLCLGGGMEIALACHMRIAATNVQMGLPEIMLGIIPAFGGTQRSARLLGNAKALEI
                     MLTGMFINMDEALRIGLVNRVYPAETLMEEGLKFASSIAKKGQIAVRAIVEAVMKGSK
                     LSLEEGLKLESTLFGKIAETHDKREGIEAFLQKRKPQFKHC"
     gene            19888..21714
                     /locus_tag="PLV81_04370"
     CDS             19888..21714
                     /locus_tag="PLV81_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOM09369.1"
                     /translation="MEEKTMNQVFENRAKKYGPRIAIEKKLKGRWESASWEDYYKRAA
                     HVGLALQDMGLKKGDRVSLLSENRLEWLYTDMGVLGAGGCIVPIYPTLIDEEVEYIVS
                     HSESKFMVVENNLQLQKGLYTAKRYPGLQKIIVIDMNGVGANPLVMSFAELLEKGKKL
                     YDANPGKFKELADAVTPDDLATIVYTSGTTGVPKGAMITHKNIMAVVMALHKIEPHYA
                     YDTDQTVPFLPLSHVFERIAGHFYGMYVGITASYAESLDTILQDIQEKRPTVVLAVPR
                     VLEKVYQRIISQVQEQSPFKQKVFYWGQKVGNKISTLREQKKKPGLLLKLKYKIAYAI
                     IFKKLQNALGGRVRWMTASGAPTAREIILFFNSAGIMVIEGYGMTECCAPATMSNIAD
                     YRIGTVGRPLPGVDIKIADDGEILIKGDNVFKGYWKMPEETKESFTPDGYFMSGDIGV
                     FDDAGFLMITDRKKDLIITSGGKNVAPQKIENLIKSDPLFTQAIVIGDKRKYLTALVN
                     ISQEQAEHVAREKGISFTSFNDLLENEQFQKVVEEHIEKCNQKLARYETVKKVGIIRN
                     EFSKETGELTATLKVKRKIVQEKYKSIIDSLYEEDAKTVQVA"
     gene            21789..22805
                     /locus_tag="PLV81_04375"
     CDS             21789..22805
                     /locus_tag="PLV81_04375"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOM09370.1"
                     /translation="MAMSVHDSDMKETMVSGKNELFTDPDPDHARAFFRNKSRSMKAK
                     LSNVKDVVRDMIKDGDYIAIGGFGANRIPTAIIHEIVRQRKKHLGFAGHTSTHDFQVL
                     VAGECIDRCDVAYIIGLEARGLSPNARRAVESGKIKLTEWTNASLSWRLKAAAMGISF
                     IPTRNILGTDTFAQSAAKVITCPFTGKKYAAMPALFPDFAAIHVHEADIYGNCRVYGA
                     SVSDFDLAKASKRVVITAERIIPHDEIRREPEKTVIPFWCVDAVIEVPYGSYPGNMPY
                     EYFSDEEHLKTWLRVEKDPKELEKFLQEQIYGVDNFYDYLNLNGGIEKMKKLRAIEYL
                     INNK"
     gene            22815..23582
                     /locus_tag="PLV81_04380"
     CDS             22815..23582
                     /locus_tag="PLV81_04380"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010038738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOM09371.1"
                     /translation="MEYNAMELMICVAARNLEDGATVVVGTGAPCAAAMLAQKLYSPN
                     LCIMFEAGGVAPILPTMPISVGDSRTFHKAIMASSMPEVMETCQRGMVDYTFLGGAQI
                     DMYGNINSTMIGDDYEKPKVRFPGSGGANDLASLCWRTMMMTVQDARRFTDKLDFITS
                     PGYLQGGRSRYEAGLPEGTGPYRIITNMAVMGFDEETKWMTVLSINPGYSRKDVQDNC
                     GFELKWAKKIEDTKPPTDEELRILREEVDPHRYIIGR"
     gene            23593..24054
                     /locus_tag="PLV81_04385"
     CDS             23593..24054
                     /locus_tag="PLV81_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HOM09372.1"
                     /translation="MEDIFKGIEPQVYKSQISVPYQWWAGETASKFFITLRDEKKILG
                     TECPQCAKVFCPPKKTCPFCYVDTKNWKEVGPKGKVITYTVVYRQLAALKKNVPVAYG
                     LIQLEGSANAMLHYIEGIEPEKISKGMEVEAVFADEKSASIHAISYFRPVK"
     gene            24065..25204
                     /locus_tag="PLV81_04390"
     CDS             24065..25204
                     /locus_tag="PLV81_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOM09373.1"
                     /translation="MEAAIIGVGMTTIEKSKVGETYADLAWEAVNKALNDAGLGIDDI
                     DNVITTSSDFWDGRTISCMAVGDASGVRYKNCSAVEGDGTFGAAYALARSLSGDYKTT
                     LVTAHSKGSESISSIITNAAFDPIYERGLGMDMISACAMQARAYMHATGTTPEQLALV
                     SCKNHKNAKDNPYAQLPMDITVKDVLSSEMIADPLHKLDCSPVSDGCCAIIIANADFV
                     KKIKAKPVWIKGVAFCSDAYFMGDRDLSKAKALEEAAKKAYSMAGITNPQNEINVAEI
                     YDAFTYQELMWIEGLGLAEYGKAGLMLEKGIFNRDGKLPVNPSGGLLSGHPVIAAGLY
                     RIAEVVLQLRGEAGGRQVNAKRGVAHGVNGLCGQSHCVWVLDKEK"
     gene            25214..26377
                     /locus_tag="PLV81_04395"
     CDS             25214..26377
                     /locus_tag="PLV81_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOM09374.1"
                     /translation="MGKRRVAIVGTGQTHHRSHRPDVNGRELIHEAVTRALEDADLSI
                     KNIDAVIIGNMDHFEGINYVDCWSIDGSGGYHKPVIKLTTGGTTGSTLAIGGYHFVAS
                     GMFDKVLIIGWEKNSESDTTGAITTAFDPVWDRLVFAGAISGLAVEAQSYMAKYGATD
                     RDGARVSVRDRKHACNNPYAQLRKEVTLEEVLASPMLADPIHLLDVCPRTDGACAVIM
                     ASEDYAEKITSTPDWIWATANRHKYTYLGDVNWGELDSMAEASKDLFKKCGITEPRKQ
                     IDVIEMYQPYSFAGIIWIEDMGIVPRGKGAEYIWSGATDMGGELPINPSGGVIACNPI
                     GATGLIRCAEAALQVMGKAEARQVPDVNFAVSTGFGGCMWTDMLLHGKKKPNL"
     gene            26387..26851
                     /locus_tag="PLV81_04400"
     CDS             26387..26851
                     /locus_tag="PLV81_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HOM09375.1"
                     /translation="MENIEYIIIESGKAVQPFNYSVGMYGSKFFHELKNNRKFVGVRC
                     PKCKKVYVPPRKVCGDCFVEMKEFVEVGPKGTIGTFTILRFAFIDPETGTQKPIPYGY
                     GFIKLDGADTLFQHYIDVHDESKVKIGARVEPVFSDEKKGNIKDILYFRVID"
     gene            26854..27642
                     /locus_tag="PLV81_04405"
     CDS             26854..27642
                     /locus_tag="PLV81_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09376.1"
                     /translation="MSIIELHNDVRAVIDERAFAMVLITGTDKVRSGYGNGDSLLVDF
                     DHRVFAVADATERFPHASRLLLSNIVSGLQTIPQSSDEWLHYLNGIYASQEYHLKSTL
                     SLIALQKQESVSRAFIAHGGDSSLQIYNKQNKSLVYKTSVNMNFAGRSKHVDSIDCIP
                     LQDQLRVILYSDGFHDVIKHCNIVMEDLLHVSLIEAITHIIDKVGNLDCEEYDDISLL
                     LFDIPEIYTVNTKGILMGGTTRLQEQKVKESISIHDYNSLIRIM"
     gene            27697..28311
                     /locus_tag="PLV81_04410"
     CDS             27697..28311
                     /locus_tag="PLV81_04410"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HOM09377.1"
                     /translation="MKIKERSDIFDIRRAQLTSATYNVVSKKGYYNFTIKDIAQEAGM
                     STGLIHYYFKNKQDLLLNLLREINRNIRTSLQHDLEHLSDPVDRLAAFIERACNLVMQ
                     EKNYFYVLLDFWTQLNHNERMRTAIQKLYTSYRDVCSEILNEGIEQGKFNNIDIRYTA
                     TMIVSMVQGLIIQYVIDPDAFDYAHYAHKIKNQIIETILNKGGQ"
     gene            28311..29459
                     /locus_tag="PLV81_04415"
     CDS             28311..29459
                     /locus_tag="PLV81_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010197461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOM09378.1"
                     /translation="MKFGFSEEQLLFRDTIYKYAKNEIVPLCEEADLKSEFSFEIWKK
                     LGQMGLLGLPFPEEYGGQGADVVTCCLAGEALGHAGVDSGHLLALGAHTYLCTDTLYK
                     HGNEAQRKKYIPKLASGEWIGCMGLTEPGAGSDAASLQTTAVKKGDRWILNGSKTFIT
                     NAPVANVCVVYATVDKSKKHAGITAFIVEKDFKGFSTGKPFHKMGVRASTTSEVFFED
                     CEVPEENVLGNIGEGFMYAHQTLEWDRSALLAPFVGGMQFMIEQCARYASERVQFEKP
                     IKSFQAIQHKLADMKVVLEAARLAVYRVASLKDAGYPINHLQASIAKAFVGDWGFKVT
                     SEAVQIFGGYGYIHDYPVERMLRDAKLAQIGGGTSEVQRLIISRLLAL"
     gene            29486..30655
                     /locus_tag="PLV81_04420"
     CDS             29486..30655
                     /locus_tag="PLV81_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOM09379.1"
                     /translation="MNYEFNDIQKSIKENFKKFCENEIKPHAALLDIGDEKSVEILKK
                     NIKKLGDLGYLGMIHEEEYGGTNQDLINFAIAGEEVAKACAATFLSAGASCGLCGMPI
                     RLFGSKKQKEKYLPGIISGDIIGSFGLTEPQAGSDAVAIATTAKKKGDKWILNGTKTF
                     ITNAPIADVCLVMAYNDKEAGPANGVTAFLVDKGTPGFSTGKPFEKLGYRGSPTSEVF
                     LEDCEVGEDAVLGEVGKGFIQAMQTLEFGRIGMATVSLGVAMACMEEAVKYSAERKAF
                     GKPINRYQEISFKLADMMILTDLSRLLIYKAAWAKQTNNPEAAILASCAKLFASEAAT
                     QISSWALQIHGGYGYIKEFPVERLYRDAKLGEIGEGTSEIQRILIARDLLQKYGS"
     gene            30673..31110
                     /locus_tag="PLV81_04425"
     CDS             30673..31110
                     /locus_tag="PLV81_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HOM09380.1"
                     /translation="MAIDKKYIGKQWPTMVYEVGKEKVKEFAKAIKSTNPYFLDDEFA
                     EQSKYKVIVAPPTFAVVFGAKLIEPIFFDNELNLNLAMLVHGEQEFEFYNVVKSGDVL
                     YSDAKIVNIENKEKLDVIAIEIMTRNQEKKDVCKGIYTFVVRK"
     gene            31113..31520
                     /locus_tag="PLV81_04430"
     CDS             31113..31520
                     /locus_tag="PLV81_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOM09381.1"
                     /translation="MKTMDSHKTISVGDSFKVNQEVDKYRAIYYAGASGDFNPIHIDA
                     EFGKMVGLGGAILQGLCTLGFTADAITTWVSDPGRLKKLKCRFASPVRMGDTLTIEGT
                     VSEVQENRAKLTVTVKNQDGIEVLTKVQAEIEV"
     gene            complement(31693..33576)
                     /locus_tag="PLV81_04435"
     CDS             complement(31693..33576)
                     /locus_tag="PLV81_04435"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOM09382.1"
                     /translation="MTFLNKTMHSKRTFYSSAILPFTIIFLQLCKSIVLYPQLYQETI
                     FNKNIVYNYFQIALTNNIAPVVIEIIQICILAPFSVALLLPVFTYLTKRKNDIIDILI
                     ILFNRWKFVATALFIAVIVLIVTVTGTISYKNTHEKPNIIILASDALRPDHFSGFGYH
                     RKTTPNIDTLIAQGTTCTNVYTVVPRTFPAWVSILTSQLPAKHGIGHMFPTAQSRNRN
                     FVTLATVLKDYGYYTSVVGDFAADIFPRINLGFDTVKAPTFNMRVMIKQIILKNHTFL
                     LPFLTNRIGFTLFPEIREFAEFADPQFVIDDIKSEISKSIHTKKPFFITTFFSITHFP
                     YPACYPYYNYFTDTSYKGPFKYSKNRVVSLDSSGVASEYEPSLQEIEYIRALYDGCLY
                     AFDEAVGAIMQYLKDKNLYDNTIIIIVSDHGENLYEHEYGMGHGEHLRGKYSLIIPCI
                     FSGPGIPHQAYTAVRSAIDITPTILDLLNIQQPQSFDGTSLMKNHNRTFDAYCETGIW
                     FDNTGNFFFQKKRIMYPDITGISTIDFNHHDEITVTPYYDNLITIAKHRSIVANNLKL
                     IYMPTHYGIEYELYDISQDPEERNNIYKTSPYANSMKQKLFLLFNNDRKIKIYNSYFM
                     PVE"
     assembly_gap    34069..34168
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            34255..35022
                     /locus_tag="PLV81_04440"
     CDS             34255..35022
                     /locus_tag="PLV81_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOM09383.1"
                     /translation="MGGNTVLIEINNFVATLTLNRPPTNSINMSMRVELDELLNQCEN
                     NNDVRVVIITGAGDKGFSAGMDVSDVTNLHNGPDAIELFNKIDRYKKPVIAAINGYAL
                     GGGCELALACHFRFMIDAPKAVIGCPEINLGITPGWGGIQRMARLLGRSKALELILLG
                     KRLTAKEALEIGLIDKIFSAETFHQETMEFANQLAKMAPLAVQSILKGMITGIEKGIE
                     EGLKVDRECSEMLAKTEDAAEGFMAFAQKREPVFKGR"
     gene            complement(35111..35689)
                     /locus_tag="PLV81_04445"
     CDS             complement(35111..35689)
                     /locus_tag="PLV81_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOM09384.1"
                     /translation="MAKSVKGTRTEKNLLAAFAGESQARTRYDYFASVAKKEGYEQIA
                     AIFQETALNEKEHAKRFFSFLEGGMVEIQASYPAGVIGTTVENLKAAAAGENEEHTKL
                     YPEAAKIAEEEGFPEIAFLFRKVAEVEKHHEQRYLDLMKNIQSGKVFKKDKKVQWKCR
                     NCGYIHESESAPEKCPTCLHPQSYFELLCDNY"
     gene            complement(35717..36016)
                     /locus_tag="PLV81_04450"
     CDS             complement(35717..36016)
                     /locus_tag="PLV81_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOM09385.1"
                     /translation="MEGIGYAIQKPQSEWKSPLDVFEAVYAHEQHVTSLIYNLADIAR
                     QEKDYATEVMLHWFIKEQVEEEANASSILERIKMVKDSVNGILQYDKILGKRGSE"
     gene            complement(36004..36381)
                     /locus_tag="PLV81_04455"
     CDS             complement(36004..36381)
                     /locus_tag="PLV81_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HOM09386.1"
                     /translation="MATKQLEVYKCEICGNIVEVLHEGKGELVCCGQPMKLQIENTVD
                     ASKEKHVPAVEKTAEGIKVQVGSVEHPMEEKHYIEWIQLIAGPKAYRQFLKPGEKPVA
                     LFKVDAESFTVREYCNLHGLWKA"
     gene            complement(36422..37411)
                     /gene="cydB"
                     /locus_tag="PLV81_04460"
     CDS             complement(36422..37411)
                     /gene="cydB"
                     /locus_tag="PLV81_04460"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOM09387.1"
                     /translation="MDISVYQNIWFFIIGFLLTVYSILDGFDLGIASLMPALAKNEKE
                     QKQVMDSIWPVWDGNEVWLITGGAALFAAFPFAYATVFSGFYLALMLVLFALIFRAVS
                     MEFWYYDEKRRKLWAWAFTIGSFLPSLLFGVALGNIIYGIPIDLNMNFTGSFFTLLRP
                     FPLVIGLLGLSAILLQGCTWAAYKIEGDVARRAKALMNKLIIANAFLFILAIALTIGF
                     LPSIVSNLSGWLGALVYAISLSYVYVCNRDNRSFNAFMGSSVSFVSLWIMAGAALYPN
                     LVRSTIEAYNLTIFNASSTQLTLKIMFIIALIGMPVVLAYTIYVYKVFKGKVS"
     gene            complement(37431..38747)
                     /locus_tag="PLV81_04465"
     CDS             complement(37431..38747)
                     /locus_tag="PLV81_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOM09388.1"
                     /translation="MDVVMLSRIQFGLAAGVHFIFPSLTLGLTLLVFLFEYKFYKTNE
                     ELFKTISTFFIKILGLVFALGVATGIVLEFSFGTNWAEYSRMVGDIFGAPLAAEGIFA
                     FFLESVFLGVLLFARNKVSKKVYLLSAFLVFFGSHLSGLWIIIANSWMQTPAGFAIEG
                     GRAVLTDFWAAALNPSTLVRFIHVIIAGWISGSLFAAGVAAWYIIKQQHTKEAQNILK
                     TAIIVFCIMSLVQFASGHLHAVQVTNLQPEKMATFEALWQTQQGAPMSIIGIPVESEK
                     KTYFEIKIPKLLSLLAYFDPNAEVKGRDAFPEDELPPVLLPYFSYHVMIGLGSLFALM
                     SLIALFLLLKKKLFTAQWFLYVLMFAIPLPIIANEFGWIAAEVGRQPWAVYKVLKTVD
                     AVSTVVPATHVLLSLIIFSLIYLLLVVLFITILVKIIKKGPQAESY"
     gene            complement(38822..38986)
                     /locus_tag="PLV81_04470"
     CDS             complement(38822..38986)
                     /locus_tag="PLV81_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOM09389.1"
                     /translation="MDKKYECTVCGYIYDPKEGDPDNGIKPGTAFKDLPDDWVCPVCG
                     AEKDQFEPVD"
     gene            complement(39102..39296)
                     /locus_tag="PLV81_04475"
     CDS             complement(39102..39296)
                     /locus_tag="PLV81_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOM09390.1"
                     /translation="MQFRVNDMMCNHCVMHITKAINEKDPAAKVQCNVTTKMVMVDSS
                     LDASIVMQAIKEAGYTPELL"
     gene            complement(39561..39965)
                     /locus_tag="PLV81_04480"
     CDS             complement(39561..39965)
                     /locus_tag="PLV81_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOM09391.1"
                     /translation="MEQYKHYLEAKGISPSYQRLKILEYLDQNRTHPTVDEIYTALLP
                     HIPTLSKTTVYNTLSLFVQEGLASMLVLDNVEARFDITVQPHGHFTCTRCGRVYDLDI
                     TRYVNMPESIDGHTIEQYQITLKGICKECSSH"
     gene            complement(40047..41207)
                     /locus_tag="PLV81_04485"
     CDS             complement(40047..41207)
                     /locus_tag="PLV81_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOM09392.1"
                     /translation="MKPVALNDFIYWVGGIDWDLRNFHGYMTQRGSTYNAYLIMDEKI
                     TLIDTVKYYLYDEMIERISKVVDPAKIDIIISNHVEMDHSGGLPKLLELCPNATLITS
                     PNGAKGLEAHYKKNWNYKVMKTGDSISLGKTTLQFILEQMVHWPDNMACYMPETKILF
                     SNDAFGQHIATSERFDSEYPLDIIMYEAKKYYANIVLPYDKQTQKVLDAVSTLDIAMI
                     APSHGIIWKDHVRAIVTEYNNWAANKTKEKALIIYDTMWNSTKLIAHAIKKAFEEKNI
                     PAQLYDLKTIHISDIMTEVLDAKYICVGSPTLNNNMLPTVAAFLTYCKGLAPKNRVGL
                     AFGSYGWGGQSVAQVEAVLAEMGWQLLPSIKVQYVPDEPLLGTIAQQVFEKL"
     gene            complement(41354..41773)
                     /locus_tag="PLV81_04490"
     CDS             complement(41354..41773)
                     /locus_tag="PLV81_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOM09393.1"
                     /translation="MTCVIDCSFSSSLFLPDENSSISTNFFKKYINKKSLLVPVLWWY
                     ETCNVIYIAVKRKRITPLQAQEILNLLNALKLETDTEYGLLFSTNIFNLSNLYSISAY
                     DAVYLDLSLRKKATLATFDAELKAAALKAGVPVIPAQ"
     gene            complement(41773..42018)
                     /locus_tag="PLV81_04495"
     CDS             complement(41773..42018)
                     /locus_tag="PLV81_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016945705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOM09394.1"
                     /translation="MKTKTIGSFDAKTHLSKILSNVEKGEVYVITKRGKPVAKIVPYT
                     KSENINIEEIFHKFENIRTSINKSVNIKEYIKEGRKY"
     gene            complement(42054..42476)
                     /locus_tag="PLV81_04500"
     CDS             complement(42054..42476)
                     /locus_tag="PLV81_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09395.1"
                     /translation="MIHQLRIALLVLILVISLILVVTYIFVTEKQDKDFYALEPVYQF
                     TRECEEEYFAMTGKHLDDTAKTQLLSLSYKTLYRGAQKPVLNTPKKPFTTFNPQLNNE
                     SLEAMHQSKELLYSAQLYTWCKPVCPKAAMKIKNYKKI"
     gene            42826..43044
                     /locus_tag="PLV81_04505"
     CDS             42826..43044
                     /locus_tag="PLV81_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09396.1"
                     /translation="MKAIRKIVKPVNNKIIITLKDNNEADEYEVIILPVENKNNQKIK
                     SKKKFLDLAGKINIDSKTISKLREKSIL"
     gene            43041..43424
                     /locus_tag="PLV81_04510"
     CDS             43041..43424
                     /locus_tag="PLV81_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOM09397.1"
                     /translation="MILVDTSIIVEYWKNPKREYTDVFITQDIAICGIVQAELIYGAR
                     SNKEVEKIISALECFNFIDIDKEDWNGIGKCLHKLRIKGVKIPFQDAIIAYLAVKNNI
                     PLWTRDKHFELIRDVIKELKVYKPI"
     gene            43609..44271
                     /locus_tag="PLV81_04515"
     CDS             43609..44271
                     /locus_tag="PLV81_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09398.1"
                     /translation="MNILNKLYLIGIVFIVGCGSLGVRTDAHSYKDPLYGTKLIKKIV
                     VYANIQNMQYRDQIESAFIDYFISLNYDCIAIKSSSIILPTRTYTNKELKEIFKSNSI
                     DALLVFTVQDAGYNHQQFTYNQPYQTYGNVYKSGSNSYSYNAYTYGGPQTYNFYKPYL
                     SAVADLYDVNTELKIWTVQLNSRGNAFANIDDALYKSIKTSVLKLEQDGIIKSQKNTQ
                     GY"
     gene            complement(44302..44439)
                     /locus_tag="PLV81_04520"
     CDS             complement(44302..44439)
                     /locus_tag="PLV81_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09399.1"
                     /translation="MHLAVNNRNLLNGWNGGGIDMECMPRGRNLLFIDDISFLFAILY
                     K"
     gene            45117..45297
                     /locus_tag="PLV81_04525"
                     /pseudo
     CDS             45117..45297
                     /locus_tag="PLV81_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        14714 a         7180 c         9586 g        13617 t
ORIGIN      
        1 atatatacat gaacatgaaa ataccattat caacatcatc cataaatata agcctgacct
       61 atttgttgat gttggctcta ccgatatgat taagaaagtt atggtttaca ggaaaaaagg
      121 tgaccctgta ttttatttca atattcatga aacaccaata ttgcggttag ggaggtttgc
      181 aagtctggat gattttccaa aaattgtaaa atacctcaat gcacattatg agtttaaggg
      241 atattttgga aaggcacgat tatgggtaag aaaataataa ataataaatt ttatttattt
      301 gcattgatgt ttttattaat actggttctt cgcattcctg cattgtatca tacaataatt
      361 gatattgatg aagctgcttt tgccgaattt gccaataagt ggttgggggg tgctattcct
      421 tatgttgggg tttatgataa caagccaata gtaacatatt tattttatta tatcatcttt
      481 tatttgtttg gaatcaataa cctctttgcc gttcatgtgg caacactatt tctggttttt
      541 atatctactg ttgttgtcta ctggtttatt gaaaagattg cagggatgga tgaagctgta
      601 gcttcaagtt tgttcttcat attcttaatg cacacgtatg agccaaaata tatagcaacc
      661 aacacagaga cgctctacac acttcctgta ttacttgcct ttgtttggta ttatgttgtt
      721 gtatgggaaa agaagagtat tctgtatgct gtgctatcgg gtatgtgtgc tggcatagct
      781 tttttaataa attataaagc aggtgttata gtagctgcat ttggtgtctt tagcatttac
      841 gtgctatgta aagccagtga taaaaagggt gaattgctca accagataaa aattctggga
      901 tctattggta ttggatttat aatccctgta tgtattgcaa tagtatattt ttttcataag
      961 cgagctttga atgagtttat taccttaggc tttttatata atttcaaata tattcaaagt
     1021 ggcatggtta gtgtcccgta tctgaaagct attgcacgat ttgtgctttt tggtatatgc
     1081 agtttgccac tctggttcct tatcgtaagg caactgttct atgagtatga gaaaagcaac
     1141 actgataaaa atctgatact attgctgctt atatggctgt ttggtagcat ttttgctgtt
     1201 atgttaggtt ggcgtgccta tggccattat ttcatacaat tggcactgcc tttatccctc
     1261 ctggctggat taagtataca ttctgtatct gctctgtttc ttaaacggtc gtatgtgttt
     1321 ctaacagtac tggctgtgct attgacagta tctcggatta atattccgct aacatatcat
     1381 tttttaggcg atagcaatgc attatcagat tttgcttata aaaacgttgc aataagagta
     1441 caggcaatta cttcaccaga tgacacaatg tttgtatggg gttcgggagc ttctgcctat
     1501 atttatgcaa acaggcgttg ttcgtcaaaa attattataa cggactatgt ttcggggcga
     1561 cagtttgggg tttccaatga agatatgggg gaaaatgtaa ataaatatat tcagtatttg
     1621 cgggataaat ttattaaaga gtttatgcaa aatcctcccg ctgtttttat agatacttcg
     1681 ccatcaggct attacgggta tgacaggttc cccctgaaga aatttccaga tcttagtgaa
     1741 ataattaaga ataattactc gtttgattgt aatatcgatg atatgaatat ttatattttg
     1801 aacaaatata caaaaaagca gtagatagag cgattatttt tatgaaaaat tattgacact
     1861 tacgctatat tttatatgaa ggttttcatg ttttgctggc gtagctcaat cggtagagca
     1921 gctgatttgt aatcagcggg ttgggggttc gattcctctc gccagcttta aagggtgagg
     1981 ttcccgagcg gccaaagggg gcagactgta aatctgttgg cgaagccttc ggtggttcga
     2041 atccacccct caccaataaa aaagagctat cattcaaaat tgttcgatag ctcttttttt
     2101 tttgggtttt tatccagaaa tgtcagtata attgttttag tgcgggtagt gcattaaact
     2161 gcctgattta atttttccag catttctttt atctgtgtat cggttaatcc atgtgcttta
     2221 ctaccctgtt caatagtttc atatatgcca atgtggcaac cgatacaatg aagcccatac
     2281 gaagctaaaa cctgtgctgc agtagggtat ttctggacaa gttcaccgaa tgtcatatct
     2341 tttgttattg tgtttttcat tgtaataacc tcatatgtaa ataatgttac tcaggtgtat
     2401 atagtatagc attgaattta tttcttttca gaattattat tttacaatat aattttacat
     2461 atttttttat caggaactat cgaaaaacaa attaagatta caatggtttc tatttatata
     2521 atatactttt tttaattgaa tataattaca tcattatgta agatggtaga aagtagaatt
     2581 taatccaatg tagcaaacat ccttaaaaaa gattaatgac ttcatttgca agggtatgga
     2641 tttgtatatg aaacggatga tagttgtggg tacttatgta ttattatttc ttattgttat
     2701 aatttttctt agtattgggc attatgagaa agtatatgcg gataattaca caaatgcaaa
     2761 tttatcagat tgtttgcaga ttgctctgca aaataatatc aatataaaaa tggccatgga
     2821 agatgtcaat gctgcatcac ttaatcttaa aataacaaaa ggaagcaata acattcaggt
     2881 aaatggaaca gtccagacag ttgagatatt gaaatcagat gctgtaaata gctcattcaa
     2941 tgttcccggc aaagataccc ttattggttt atttgtgggt gcacaggcaa gttataagct
     3001 ttatgatgct aataagagta actatgagcg gttacgcgag ctggaatata ataatattgc
     3061 gctattggct ttaaagacaa aaaataatat tattcgtgat gttaagatag ctttttacca
     3121 attagcattt gcaaaaaaga aaagtgaatt gctggaaaaa ttagttaaaa gctttgaaat
     3181 aaagtatgct aatgttgagc agctatttaa gaatggacaa cgaaccatgc tggatatttc
     3241 acgggcgcag gtggacctta ccaatgcacg tttgaattat caaagagctc aaaatgaggt
     3301 tgcagttgca aaaagcaaat tactgacact gatgggtata caaattgagc cttttgatat
     3361 aaacattgcc gacattgata ttgatagtac tataaaagaa ataccatata gtttagatga
     3421 tttgcttgaa acagctttgc aatattcgtt tgatattaag tcggcggaaa ttaatcagga
     3481 aattgccaga acgcagattg aaattgaaaa ggataggcgt ttgcctgtta taaatattat
     3541 agcatccctg ggatatgaaa acagggcaat tcagaatggt gcttttacag aatcgttgtc
     3601 ggatagggat aactggaagc caactatgca tgctggagtc agtgcaaatt tccctatttt
     3661 taccagtggt gttctttctt caagtattga ccgtgcggcg gtttcattta acaaagcttt
     3721 atacaacacc aaaaccgttg tggatacttt atcgggaaat attaagagtt attataattc
     3781 attgaaagat ataaaagtgc aactggatat atcacggcaa ttgattgaaa atgcaaggaa
     3841 atatctggaa ctggtgcaga agaattatga caggggaata ataacacaga atgaattaca
     3901 ggatgccgag ttgaattata ttaatgttac tatcgattat ataaagattc ttactgatta
     3961 ttatattaat tataatcaat tgataagttt attgtgtgtt gatgaaaata ttctcttttc
     4021 aaaaggggtt gatgcaaaat gataaaagaa atagtattaa ataataagaa aaaagtcctt
     4081 attgctggtg ttgtcataat catccttata ctgatatatt ctttgcgctc tggtgttgat
     4141 gattcagaat atatttatga ttacgaaaaa gtaagtattg gtgaaatatc taaaacaata
     4201 tcaacaaccg ggactattga tctgttagat cctgtttcta tattatgcca ggttaatggg
     4261 gttgttcaga aagtatatgt tgattttaat cagcatgtta agcaatatca attgttagct
     4321 ttaatagatg ccggtgattt gcaaaatgat attacaaaaa tgcagaagag gcttgaacag
     4381 ttaaatatag atctgaaaaa catggaaaat gatctgaagg tcaaaaaaag catgtataat
     4441 gaacatatga tatcagccag tgcccttgag atggctgaaa atgaatataa gaagatgcag
     4501 ttatcatata gccaggtgct ggttgattat aaaaatttac tccagatgca aaagaataca
     4561 agaatttatg ctccaataag cggtattatt gtttctcgag acattaatga atctgatccc
     4621 gttgtacgga ataagcggtt gttcttgatg gtacgcgatc taaaaaccat gcagattatg
     4681 gtagtcatag atgaaactga tatcggcaag gttaaaagtg atcagaaaat caaatttact
     4741 gttaatgcat atccggatca aacatttacc ggagctatca aacaaatacg ctttaatccg
     4801 gtgaacaggg gaggtgttat tacctatgat gctgttgcaa tatgtgacaa ctctcagtat
     4861 ttattaaaac cgggtatgac agcaatagtt accattgagg ttgataaaaa acagaatact
     4921 atgcgaatac ccaatgatgc ttttactgtt attccacctt ttattgataa atacaaactt
     4981 aataaagata aaaaatatat atggaaaaag aaaagaggta cgatagtgcc tgttgaagta
     5041 caaactggat tatacggaga tgaatataca gaaataaaaa aaggtgatgt aaaacctggt
     5101 gatactattc ttatacgtgt aagaaagcaa atttcaatta aaaagtagta attctagtat
     5161 gaatattcca attattaata ttgagaattt gcaaaaaatt tataaaataa gcgaagaagt
     5221 ttctgttcat gccttgaagg gtgttacttt aaaagttaaa agaggtgaat ttgttgccat
     5281 aatgggagcc tcaggttccg ggaaatctac atttatgaac attttaggat ttctggatac
     5341 accaacttct ggcaaataca ttttagatgg tattgatggc acatcgttaa acgataatca
     5401 gaaagctgaa atcagaaaca ggaagattgg ttttgtattt cagggattca atctgctatc
     5461 aagaaccccg gctatagaga atgttgaaat gccattgttc tacagaggtg gttttactca
     5521 tgaagagatg aaaaacaggg ctagaaaatt attggcaatg gttggcttat ctggcaggga
     5581 gatgcaccat cccaatgagc tatcaggtgg cgaacagcaa cgtgttgcta ttgcgcgagc
     5641 gcttatcaat gaccctgcaa ttctgcttgc agatgaacca acaggtaacc tggatacaaa
     5701 aaatacaact gagatcatga atgtatttac tacattaaac aaagagtata atattactat
     5761 tataatggta actcatgaac ctgatgtggc tgaatatgca gatagaaaag ttgtatttaa
     5821 ggatggcttg attattgatg acagcccaat aaaaaagttg aaaagaagta gaatataata
     5881 tggactttac aaatctttta ataactgctt tacgatcgct ttcaaaaaat aaagctcgtt
     5941 caattcttac cagcattggt attataatag gcgtttcctc ggttattgta atgattggaa
     6001 ttggacggag tgctcaaatt actgttgaag aaagagttgg tacctatggc aaaaatgcca
     6061 tggagattgc ctcatttcat accttgctta ccgaagctga tgcagaaaat atcaaaaaaa
     6121 aatttcctca ggttaaatat gcttcaatta tcattaccaa gtcaaatgtt caggtaaatt
     6181 ataaagatcg atcgacaata agcgggatgt atggggtaga aaatgattat ttcaagatga
     6241 aattatggcc attaacatca gggagatatt ttactgatat tgagatagca acacacgaaa
     6301 aagttgccat tattggggcc acagttatgc gagatgtttt tatatatgaa gagcccgttg
     6361 gcaatattgt aattattaat aatgtgcctt ttaaggttat cggggtgctg tcagaattgg
     6421 gtgagtcatt tggtggaaaa gattttgata atgtcgtggt aataccttat ggtgctgcac
     6481 agcggaaaat tattgggaac aacaagattg accgtatata tgtatcggtg tattcagata
     6541 aacagatgga tgaagcagtt gaacaaatta aagaatatct ccacagggcc catatggttc
     6601 cacctgattc aaatgagttt cgtattacaa caagcaagca aaagctagag atggcggaat
     6661 atatatcaaa aacactatct atgttactgg caggaattgc ttctatttcc ctttttgttg
     6721 ggggtgttgg cataatgaat attatgcttg tttcagtaag tgagcgcaca cgtgaaatag
     6781 gcatacgaat ggcaataggt gcaaaaaggc gtgatatact cagtcagttt ttgattgaat
     6841 cagtaacgtt gagcggattg gggggcatcg ttggcattat tctgggttta tttgtttatt
     6901 atctcataac ctatattgta gaatggccat atatattttc tgttacatca attctactgt
     6961 catttctctt tgcatgtgct gttggtatat tttttggtta ttaccctgcc aaaaaggctg
     7021 cagatttaaa acctattgaa gccttacggt atgaataaat aataaatttt ttcgttaaat
     7081 cccttcaaga cataatccat aattttccga tattatatca gaaataaaaa ataataattt
     7141 gtattattta tagttatttt atcccggtag gattattgcg aatcaaggag gagtattatg
     7201 aagaggtgat atactttgaa agatattatt gcaataaaga ggtttaagcg gtttttacag
     7261 attgaaaagg ggctttctcc taattctatt tattcataca cctatgattt aaaaaaattt
     7321 tctgattttt tggcgcgtaa aaataaaaca atactcaatg cttcacagga tgatgtggaa
     7381 cagtttttat tttttgaaaa aaataaaaaa agaaattccg cacggacatt agcgcgatcc
     7441 ctggccgcaa tacggcagtt ttataattat atggcaagca ttgagaatat tagcaaccca
     7501 actgtaaaaa tagaaacacc tcattttcaa aaagctttgc ctgatttttt gtctatagag
     7561 gaaattgaca ggctgtttgc ttcaatacgt gaggatgatc tgtaccagtt gcgtgataaa
     7621 gcaatatttg aattattgta ttcagcgggt ttgcgaatat ctgaagcagt agaattaaag
     7681 gtgatagatg ttgatttgaa aaacaagttc ttaaaagtat tgggtaaagg aaataaagaa
     7741 cggattgtac ctattggaga tgaagcaaaa cgcctgattg aaaaatactt aaatgattca
     7801 cgccctatta tacttggcaa tagaataagt gagtatcttt tcataagcaa gaaaggcggc
     7861 atgttgaatc gaaaatcagt gtggcgattg ctaaaaggtt atgccaggat ggttgatata
     7921 cgtaaaaata taactcctca tacattacgc cattcatttg caacacattt acttgaacgt
     7981 ggagctgacc tcagagctgt ccaggaactt ttggggcatg tggatatttc aacaacacaa
     8041 gtatacacac atttagcacg taagcaattg caggaaattc acaaacgttt tcatccaaaa
     8101 agttaagggt ttcaggtttt taatatggcg atagctcatg ttttcagatg gttgctgatt
     8161 ataatctttg taacaatagc aggttgtgtg cctccgcctg aatatatggc taaaactgga
     8221 aagcgtattg ggtttgataa cgattatatt aaaattcttt tattacgcga ccagcttaaa
     8281 gttgaagtga tcaccacagc caggacaaaa gtaatacaac cagcaaataa tactattgta
     8341 tataatggtg atgccaagcg ttttgtattg tttgacaatc aattgacaca accattgcag
     8401 tttgaatcct ggaataatga aattaaggta aatggtatta cagttagagg gatgatacag
     8461 cttcatactg ttgtggggaa aatacaggta gttaatgtat taaagatgaa tgaatatctt
     8521 tatggcgtct tgccatctga aataatgtca ggctggcctg aagaagtctt aaaagcacag
     8581 gctgttgcag cacgaacgta cgcatactat catctgatga aaaacaacag tacgtacttt
     8641 gacctagacg caacaaacaa ttttcaggta tataaaggta aagcagttga aactgaagca
     8701 accaacaaag cggtggatgc tactgctggt attattatga catataataa taaacccata
     8761 cttgcatatt tccattcaac ctgtggagga aaaactacag atgacaaata tgtatggcag
     8821 ggtgaggatt tgccatatct gacatcagta gtttgcccgt attgtaaaaa ttctcccaat
     8881 tatagctggc aggttgagct taccttaaat gaaatatatg aagctctggt caaaagatat
     8941 aagggagtag gaaaaattaa ggctataacc ttaggacgcc aaggtacccg tgtaacagtg
     9001 gttaagatag aacatgataa cggcattatc agaatgagcg gaaatgattt caggttaatg
     9061 tttgatgcaa aaaagataaa aagcctgtat tttgaagcaa aacaaacgcg gacaggatta
     9121 attttgacgg ggcatggatg gggtcacggt gtaggaatgt gtcagtgggg tgcaaaagag
     9181 atggcacaac agggtatgcg ttttgatcag attcttaagt tttattataa aggcatacga
     9241 atcatgacat attctatgca aaagtaaaca tgttatcttt gctctgaata atagaattgc
     9301 cgggaacatt atcacacctg gataaggatt ggtagccgca cttcgattga attcagtaat
     9361 tgcttttcaa ttaaagatta aacttaatca atcatgttat gattgtatca tcttcaggta
     9421 ttccttcacc gtataatctc aatgcttttt attaactaca tttgcaacca ttgataaacc
     9481 ctgcaatcca ttacatgaac tatcgcacat accaaaaaat tacagtgaca atagtattaa
     9541 atataatctt gactttgata tttgttcatt gtaaaataaa catatgccag gaggaagtat
     9601 atgaaaaaga tgagtatcag cgttcttttt atactctttg gaatatgcgt tttggctata
     9661 gcgcaaacac aaaaatttgg tgaagcatta cacggattat atgcatataa agattttcaa
     9721 aaggcaacat actgtcgtca atgtcatgtg gaaatttatt accaatggca aaagagtatg
     9781 atggcacagg cttttgttca tccatgggat gagattgaat attttgattt agctgtttct
     9841 catggcaggc gaaatccaaa atttaaacct gttgctgatg gctgtaatgg atgccatgcg
     9901 ccgatttcat ttatggcagg agatacacct ccgccacggc cagagaaaaa atcaatggca
     9961 aatgaaggcg taagttgtga tgtatgtcat accattgtgt cgtttaatca gaatgaacag
    10021 tataacttca gtttctttgc aaagcctgga agaacaaaat atggaccaag aggaggcaaa
    10081 aattctccag cacatgatat cgtaaaatca gatttaatta ccaagcctga attttgtgcc
    10141 aattgccata atgaaaaaag cccatgggga gtatgggtta aagcaacata taatgagtgg
    10201 aaagagggcc catatagcaa agagggggta gtgtgccaga catgccatat gcctaaagcg
    10261 gcaggcaagc gagcaaagac tgaaaacaaa ttatacgatg atcttaaaca gcatacattc
    10321 catggaggcc attttcaggc caaatacaat ggtgcaatag atgttctgat atatcctgat
    10381 acagattttg ttgaaccagg gatgaaaaca aagatatcag tttatctgta taatcaaaag
    10441 tgtggccata aagtgccaac tggttcagtt gaagacaggc ttttgtattt gcatgttgaa
    10501 gcaattgaca gcaaaggaaa tgtatatcat ctgaaagttg ataaaaaagg atttcaaggg
    10561 gaagaataca ctattgcttc acgtgagaag gcatatcagg attttgccga gatgatggat
    10621 gtaccagcag attttgatgg cataatccgt gaagatgttc cagttgggga tagaatattc
    10681 agaatgcctt attttactga aaatgggatt atgactattg ctcagtggaa tacagcaaag
    10741 tttggtgtgg attacagaat agcgccacgg gaaacaaaag ttgaaacata tacatggacc
    10801 gtccccgata ctgttgcacc ggggccattg acaatacggg caacattgta ttatcaatta
    10861 ctggtaagac cagttgcaca atttttaaaa gtgccggagt cagagtcaat ggatagaatg
    10921 ataaatactg atgttgtaac aattgatgtt atatattaaa taaaagacag ccaacgggag
    10981 gagcagtgca atgaaaacat gtaccatgta taccattatt gtattattgg ttattggtgt
    11041 actattatat tgtgaagaac aatcacaggc aatgccagga tttgcacgca ggtataagtt
    11101 tacctgccag acatgccatg cgccttttcc caaacttaaa ccttacgggg aagaatttgc
    11161 tgcaaatgga ttcaggctgc ctgaaggtga gccttcatat gcaacaatag ataccggtga
    11221 tccttcactg gaattgacac gtaatttccc tattggcatg cgactggatg cgtatgcagt
    11281 tgcagataaa gatgatgaag tatctaatga tctacaaaca ccaatactat tgaaatttat
    11341 ttcgggtggc cctatcgcac agaatttatc atattacttt tactttttct tttctgaaaa
    11401 aggggatgtt gttggtattg aagatgcttt cttgtattat actgatttat tgggaacagg
    11461 tattaatata acggcaggtc aattttctgt atctgacccc atctacaaga atgaactcag
    11521 attgacgtat gaaagctatg ttccgtttgc tgcaaaaccc aatcgttcat tatgcaattt
    11581 aaaatatgat cgtggcataa ttgttgataa aggctttgac tttggattaa cgttagttgc
    11641 ggagatagta aatgggaatg ggattgatcc ggcagatgaa catgataatt ttgatggcga
    11701 taagtataag aattactttt ttagagccag tcaggaactt tttgatgtgg taaggattgg
    11761 tggttttggt tacataggta aagaaaaaaa tacagcagat gatactgtta ccgatgtgtt
    11821 cacgaatagt gtgtcttatt atggtgggga tttgtcaatt attttgggac cgtttgagct
    11881 taacacatta tttctgtacc gaaaagatag taatgcatat tttactgaaa ataaaaaatc
    11941 aattgccaca aaaggttata tggcagaatt gatatttcag cccgaaggtg accgcagcaa
    12001 ctatgtgatt acagcactat acaataaggt agattcagat tatcgggaaa gtggagtcca
    12061 ttcattaaat tacgaaagtg caacactatc gttaacatac ctcttggcac gaaatgtaaa
    12121 aataataggt gagtatacct acttattgca tgattattca aaaggtgagg attataaaaa
    12181 ccatcatata ttgaaaacag gtgtttcggc tgctttctaa aagataattt atatttttat
    12241 tgtgctataa ttgctatcaa gataattgtc aaacatggaa aagaaattat atcttttaga
    12301 aagctacgat tttgacctcc cacaggagtt gattgctcag tacccggctg ataaacgtga
    12361 agaatcacgt ctgcttattc ttgatagaaa atctaaacac ttggtgcatg aaaaatttta
    12421 caatttacct aactacatta atgccaatga tattctgatc ttcaataatg ctaaagttat
    12481 accagcccga atttattgta aacgctcaac aggtgctacc atcgaagttg tcttaacaaa
    12541 aagattgtgc gataatacct gggaagctat ctgcaaccgg ttaaaaagga ttaaacctgg
    12601 tgaaatactg tatcctatta aaaataattc agtgtcgata gttgttgtgg aaaaatcgga
    12661 agaaggtatt acaataaaat ttgagcctaa catagattat gcaatgcttg aaaagatagg
    12721 agagttgcct ctaccaccct atattcaacg gtcccctgat aaaaatgatt atgaacgcta
    12781 ccaaacagta tttgccagga agtacggtgc agcagcagcg cccacagcgg gattgcattt
    12841 cactgatgat ttgctgaaac agatacagga taaaggtgct actatttcat ttttaaccct
    12901 gtatgtatca tggggcacat ttcaacctgt tagagtggat gatattaggc tacatacaat
    12961 gcatacagaa gaatactatt tgccagaaaa aaccgtggaa tctatctata atgcaaaggc
    13021 aaaaggagga agggtgatag ctgttggcac cacatcactg cgtgtacttg aatcagttta
    13081 tcataatggt gagtataagc atggatatgg agctaccaac atttttattt atccacccta
    13141 tactgtcaaa tctgttgatg cgctgattac caattttcac actcccggat caacattgct
    13201 tatgcttgtt gctgcgtttg ctggatatga ttgtattatg caaacataca gggaagcagt
    13261 taaggaacga tacagatttt tttcatacgg tgatgcaatg tttattacat gatgggacga
    13321 gttgtacatt attatttatt tgtacataag ttgctgttaa aaaaataatc atccaaacag
    13381 aaaaattttt aaaaaattat tgacatattg tattatatta attaatttgc aaattgattg
    13441 gatgtccaat aaattatatg ttgcttcaaa tttttatttt tttatcaaaa tattggttgg
    13501 acattcattc aattgtttaa aaaattaatt aaattgtata atacaatgct tttgaaaggg
    13561 tgcctacata cgcataccac atgttctgat gggacgctta caccccagca ggttgctgtg
    13621 cgttatgaag aaaaaggata cgattttata gctttcaccg atcacgatta cctgttaaaa
    13681 cccggctatg atgaaatcta taataatgtt aaatcaaata tgattatttt tccaggtatt
    13741 gaaatgactg tgttcttgaa aggatatatc cacgtaaaca ggatttttgg tgataatcag
    13801 ttgcttcaca tatttaatca tctgccagag tatgaccttg atatagaaca gattatggat
    13861 aggctttata atcttcagaa aatgtttgcc attgatgcta tggaaataac ttccaaagga
    13921 tttaaggtta aacggttcaa cgaaatgaca tttccgcatc cttcaatagt ctctgatgat
    13981 gctcacacag ctataggtat tggtagggca tggatagaac tggatgcaaa aaggaataag
    14041 gattcaatat taaaagccat caaacatggt gatttctgga actgctattg ttaggcatgg
    14101 gtataatgtg aatttaccat ataatattta atatttaaaa aggaggttca tagggtatgc
    14161 aaaaacgagt tgctatctgt gctgttgctc agattaagaa tgaccccaat ttgtggtatg
    14221 cgcgtttcca gaatatgctt ctggaatgtt ttgaaggcat aatgtcacaa acaaaggtga
    14281 gctttgatat ggaaaagggc atacgtactg ttattacctg ttctgatgac gtgtgggatg
    14341 cgcgtacaat ttcaaataat ggcatgactg atgttgttgg tgctcatttc aggggtgaag
    14401 agaagatggc gcaggaaagc cttaatggtt tggggtatgc tatggcaagc atactctctg
    14461 ggcatgatga tgtggttctt tatatgggac atatgaaaga atcgcagcca gaaagccgta
    14521 ctatgtgtac aaatcttgca tttgacccat tctattgcag gccactgggg atggattttg
    14581 ttaatgtcaa tgcaatgcag gcacgggcat atatggaaaa gtccggggta aatgaaaaac
    14641 atctggcaaa gattgtggtt cgttcaaggc aaaaagctaa gcaaaacccc tttgcacgtg
    14701 aaaatgaacc aataaaagaa gatgatgtta tgaagtcgcc tctattagct gatccactgc
    14761 ggcaactgca ttattatcca acaaccgatt gggcttttgg catgttgctt tgtagtgaag
    14821 aacgtgtaaa agaatttacc gataagccag tgtggataac cggctatgca agctgtatgg
    14881 atagttattt ccttggtgat aaggatttaa catcaaattt ttcattacga aatgcaactg
    14941 aaagggcatt gaagaaagca gggatcaaag atgttaaaaa agacattcaa ctttttgaat
    15001 tgtgtgacca tgctgcatac cagttgccaa tgtgggctga aggcattggt ctggctaaag
    15061 aaggcaaagg cgggaagtgg attgatgatg gtggcatgga tgaatttaat gtcaatatgt
    15121 ctggaggcat gttaaatggc aatccattgc ttttaggtgg tgctgccaga gctatcgaat
    15181 gcttttatca gctaagaggt gaagctggta aacgtcaggt taaaggtgca aaacgtgcat
    15241 tggcacagag cacctatggt gcggccggtc aacaccaggc tgttgtgatt tttgaagtgt
    15301 aaaggagggg tgaccatggc acaaaaaaag gatagaaatg ttgcaattat tggtatagga
    15361 caaaccaagc attcaagcca tagagaagat gtcaatcagc ctgagatgat ccatgaagca
    15421 gtatctgctg cgcttaaaga tgctaatata agcatcaagg atgtagattg tatagttcat
    15481 ggtaatatgg aactatttga gatgattcac cagcctgatt tatggcatgt gcttggaaca
    15541 ggagcgtatg ggaaggacag catcagaata actactggtg gaacggtggg tacaacgctt
    15601 gcatgcgcca gtgacaatct ggttgcgtca ggaatgtatg acatagtcat ggcgattgga
    15661 tttgagaaac tgcaggaagg tcatacaacg ggtggtatta ccaatatggc tgatccccta
    15721 tggttccgaa atttgcagac aggtgcatta acaggatcca ctgcatatga tgtcattcag
    15781 gagtttggtg aagatagagc aaaacgtgca gcaatgatgt atcgtattat catggataag
    15841 catgcatgtt taaatcccaa tgcacacagg gcatttggcc tggattttga tcagcttgaa
    15901 acattgatga acacatcgcc gcaactggtt ggtgaattgc gccttattca catgtgctcg
    15961 caatcagatg gtgcgtgtgc agtaatcttt gccagtgaga aaaaagctaa agagctatct
    16021 aaaaaacctg tatggatacg ggatcatatt actatccacc gtgaagaaac cttcaatata
    16081 tttggctatc acaaagatta tcccgtgttg aagacacata aatttgctgc tgaacaactc
    16141 tttaaaagaa atgacattaa aaacccgctg gaatatttcg atgtatttga aatgtatgac
    16201 cctgctgcat ggtggggact ggattggctg agagacttct tcttcctgaa aggtgatgaa
    16261 caccttcgtt tagtagaaaa taaggagatc atgattggtg gttctatgcc aatcaatcct
    16321 tcaggaggtg ttattgcaac taatccaatt ggtgctactg ctttattgcg ggttgctgaa
    16381 gcagcgttgc aggtcagggg tgatgcaggt gctcatcaaa ttcccaaaga agttaagcat
    16441 gcgttagcct ctgggtttgg tggtaccatg tggactgttc tgatgatgtt agaaaaagaa
    16501 ttaagctggt aggagggtta tatggaatac tggggtgtta aagtagaaga tatatttgat
    16561 acaatgaacg accgattcag accggaaggt gcaaaaggtg ttactgctaa gtttggatat
    16621 gatattaccg gtgaaggtaa atggaaaatc attgtatccg atggcttgct ggaagtcatt
    16681 aaaactgatg atatcagcga ttgtgttgca gtgatggttt gtgacggaga gacatttgtt
    16741 ggaattaaca ttggcaaagt tgatgcggca aatgctttta cgtctggcaa ggtaaaaatt
    16801 gagggtgact ttggccagtt tggcaaaact gcaaagatgt ttaaaaagta tgtcccaaag
    16861 aaagctgcca tgtcaacacg ggattacatt cttgatatgt ttggtacact tgtatcccgt
    16921 ttccagccac agaatgcccc tcaggaggct gtcatagtgt atgatatagg tggtgctgat
    16981 ggtggtgtgt ggacagcata cattaaagat ggcaagtgcg aattaaaaga agagcgggct
    17041 gaaaaattta ccgttaaact tgaggttcag gaagctaaag attgggtaga tgtaatgttg
    17101 ggtaaagttg atgcgttttc actactatca gcaggaaagg cagcgatagt tggtgaaaca
    17161 cagctggcat taaaattggg tgagatattt ggaaaatatc aggacccaat ggcaagcggt
    17221 acaccagaac aggaactcat tgtactgaag aagataatat ctgttgatca gaagttttca
    17281 acaggacctg tgatggggaa attcctgaat gcattgaaag aaaagaagat ccttggtaac
    17341 aaatgcccca aatgcgggag aattcaactg ccagcaagag aagtttgtgc agaatgccgt
    17401 gttcgtgtgg atgaatgggt tgaggttggc ccaaagggtc aggtgcgtta tatggaatat
    17461 gtgtattatg ccagccctga tccgttaact ggtgaaacac gggaaacacc gtatggaatg
    17521 gtaaatatac tgttagatgg ttgttcgggt aatgatacgt ttgcccattt gatccgcagg
    17581 gaccagatag acaggataaa aggtggtaaa gatgatgttt caggtacacg tgtacgcccg
    17641 gtgtggaatg aacaccgtac tggaagcatc tttgatatca aatattttga gattgatgag
    17701 tgatggagga atgctatggc acttgattat gacaggaaag attgttttgt agttgaagga
    17761 aagttagcat tgccttatag ttattttgct ggaagggtag gtagtacctt tattaccacg
    17821 cttcgtgaca agaaaaagat catgggcgta aaatgtcccg tgtgtaacaa agtatatctg
    17881 ccaccacgcc agacatgtga gcgtgatctt actgatatac gtgataactg ggttgaggta
    17941 cagccaactg gtgaggttgt aaattttacg gttgtacggt atgatgacaa gcatcttccc
    18001 aggaaagcac cctttgtgat ggcacttata aagcttgatg gtgctgatac gcctattgtt
    18061 cacattcttg atggaatagc acctgaagaa gtaaaaattg ggatgaaggt tgaggcagta
    18121 ttttcatctt cacccacaaa taccatttta gatatttctc attttgcacc taagaaacct
    18181 gaaaaagcat ttgtgtctga acgtaaacct gcaggtgcaa agggagagga gccagtaatt
    18241 tcagaagagg aaaaattgtt aaaagaaagg aggaaagcta tgtccaaaaa agtcattatc
    18301 accgcagctc tggctggagc agcaacaatg aaaaaccaga ccccatcagt tccttacaca
    18361 ccacaggaat ttgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatgaat
    18481 ttcagcatta tagaccgaaa gactggaaaa atatttatag actttgtgtt tgagaatacg
    18541 ttcaccatgc tacaggactt tgggaaagcc atggaagaaa atggagtaaa acctgaagtt
    18601 gaagtgtatg acctgggtgg cctggataac tggcttttga tttcaaaaca gggattcttt
    18661 acaaaaccat ataacttcaa ctttgtatgg ggtgttgcag gtggtatggc attcaggccg
    18721 gatatgttta tggttttaaa gaacgcatta cccgaagatg ctaactttac tacctgtgga
    18781 gtaggtctgg aacaattccc tgcaataact atgtcatgtc ttgttggcgg ccacatgcgt
    18841 gttggtcttg aggataatat acggatacct accggcgaac tggctaaagg tagctatgag
    18901 caggttgagt gggcagtgcg tatagctgaa tcattgggca gagagcctgc cacacctgat
    18961 gaagcacgtg aaattatggg attaaagaag cgatagttac tatcacagta catccctgcc
    19021 ggtgaaatta tcctgcaggg gtgtactgaa aatcagttct gcattcactt actgttagag
    19081 gttatttgta tgggtaataa atgttactat cagatggaaa aagaaaacgt agcagtagtg
    19141 tattttgaca atccaccttt gaatgcactt gatacagaaa ccatggaaca gtttgaaaat
    19201 ataatgagtc agctggtgca ggatacttct gttcatgcaa ttgtattgac gggtaaaggt
    19261 tctacatttg ttgttggtgc ggatgttaat gcggttatgg aagtggatac ctatgaaaaa
    19321 ggagtggaga taacaagcag ggcacaggca atagtatctt tatgtgaact atcgccaaaa
    19381 cctgttattg cccttataaa tggtttatgt ctgggtggtg gcatggagat agcattagcg
    19441 tgtcacatgc gtatagcagc tacaaatgtg cagatgggat tacccgaaat catgctggga
    19501 ataattcccg catttggagg tacacagcgt agtgccaggt tattaggaaa tgcaaaagca
    19561 ttagaaatta tgttaacagg aatgtttata aacatggatg aggcattacg tattggactt
    19621 gttaaccgag tatatccagc agaaacattg atggaagaag gtttaaaatt tgcctcatca
    19681 attgcaaaaa aagggcagat agctgtgagg gctattgtgg aagcagtaat gaaaggaagc
    19741 aaactgagcc ttgaagaagg attaaaatta gaaagtacat tatttggaaa gattgccgag
    19801 acacacgaca aaagggaagg gatagaggct tttttgcaaa aaagaaaacc tcaattcaag
    19861 cattgttaat acaatttgga ggattttatg gaagaaaaaa caatgaatca ggtttttgag
    19921 aatagagcca aaaaatatgg accgcgaata gcaattgaaa agaaattgaa aggcaggtgg
    19981 gaaagtgcct catgggaaga ctactataaa cgcgctgcgc atgtaggact ggcattgcag
    20041 gatatggggc ttaaaaaagg cgacagggtt tctttgctat ctgagaacag gcttgaatgg
    20101 ctctataccg atatgggagt gttgggtgct ggaggatgta tagtacctat ctatcctaca
    20161 ttaattgatg aagaagttga gtatattgtt tcccattctg aatcaaagtt tatggttgtg
    20221 gaaaacaatc tacaattaca aaagggatta tacactgcaa aacggtatcc aggattacaa
    20281 aagattatag taattgatat gaatggcgtc ggtgcaaatc cactggtaat gagctttgct
    20341 gaattacttg aaaaaggtaa aaaattgtat gatgctaatc ctggtaagtt taaagagtta
    20401 gctgatgcag taacgccgga tgatcttgca actatagtat atacatcagg aacaactggt
    20461 gtgccaaaag gtgcaatgat tacccataaa aacattatgg cagtagtcat ggcattgcac
    20521 aagattgagc cacattatgc gtatgatacc gatcagactg ttccattttt gccattgagt
    20581 catgtatttg aacgaatagc cggacatttc tatggtatgt atgttggcat aactgcttca
    20641 tatgcagaaa gtttagatac tatcttacag gacattcagg aaaaacggcc aacggttgtg
    20701 cttgcagtgc cccgtgtgct tgaaaaagtt taccagcgaa ttatatcgca ggttcaggaa
    20761 cagtccccgt ttaaacagaa ggtattttac tgggggcaga aagttggtaa taaaataagc
    20821 accttgcggg aacagaagaa gaagccgggg ttgttgctga aattaaagta taaaatagcg
    20881 tatgccatca tatttaaaaa attgcagaat gcattgggtg gtagagttcg ctggatgaca
    20941 gcatcaggcg ctccaaccgc acgtgaaata atattattct ttaattcagc aggtatcatg
    21001 gtcatagaag gctatggaat gactgaatgt tgtgcaccgg ctactatgag taatattgct
    21061 gattaccgca ttggtacagt gggaaggcct ttgcctggtg ttgatattaa aatagctgat
    21121 gatggtgaga tacttattaa aggcgataat gtgttcaaag ggtactggaa gatgcccgaa
    21181 gaaacaaaag aatcttttac acctgatgga tattttatga gtggtgatat tggagtattt
    21241 gatgatgcag gatttttgat gattactgac aggaagaaag atctgattat tacatctggt
    21301 ggtaaaaacg tagcaccgca gaagattgaa aatcttataa aatcagaccc attgtttaca
    21361 caggcgatag ttattggtga taagcgaaaa tatttaacag cactggttaa tatcagtcag
    21421 gaacaggctg aacatgttgc aagagaaaaa ggtatttcat ttacaagttt taatgattta
    21481 ctggaaaatg aacagttcca gaaggttgtg gaagagcata ttgagaaatg caatcaaaag
    21541 ctggcacggt atgaaacagt gaaaaaggtg ggtattatac gaaatgaatt ttcaaaagaa
    21601 accggtgaat taactgcaac actcaaagtt aagcgtaaaa ttgtacagga aaagtataaa
    21661 tccatcattg attctctgta tgaggaggat gccaaaactg tacaggtagc ttaaaaacga
    21721 atatatttga ttgtatgttc aaccaatatt ataatgtatg tataatttat taggctgagg
    21781 aggaaactat ggcaatgagt gtacatgata gtgatatgaa agaaacaatg gtatccggta
    21841 aaaatgaatt attcactgat ccggatccag atcatgcacg agcttttttc aggaataaat
    21901 cacgttccat gaaagctaag ctatcaaatg ttaaggatgt ggtgagggat atgatcaaag
    21961 atggtgatta catcgccatc ggcggttttg gtgcaaaccg tatcccaact gctatcattc
    22021 atgaaatagt acgtcagcga aaaaaacatt taggctttgc cgggcacaca tcaacccatg
    22081 attttcaggt gctggtggca ggtgaatgca ttgatcgctg tgatgtggct tatattatag
    22141 gcctggaagc gcgaggccta tcgcccaatg cacgccgtgc tgtggaaagc ggtaaaatca
    22201 aacttactga gtggaccaat gcctcacttt catggcgatt gaaagccgca gcaatgggaa
    22261 tttcttttat tccaacacgc aatattctgg gtactgatac gtttgcgcag agtgctgcca
    22321 aagttataac ctgcccattt actggcaaaa agtatgcagc aatgccagca ctgttccctg
    22381 actttgcagc cattcatgtt catgaagcgg atatctatgg gaattgcagg gtgtatggtg
    22441 catcggtatc tgactttgat ttagcaaaag cttccaagcg agtggttata actgctgagc
    22501 gtattattcc acatgatgaa atacgccgcg agcctgaaaa aacagttatt ccattctggt
    22561 gtgtggatgc agtgattgag gttccgtatg gcagctatcc aggcaatatg ccttatgaat
    22621 acttcagtga tgaagagcac ctgaagacat ggctacgggt ggaaaaggac cccaaagagt
    22681 tagagaagtt tttacaggaa cagatttatg gggtggataa tttttatgac tatctcaact
    22741 taaatggtgg gatagaaaag atgaagaagc tcagagccat tgaatatttg attaacaata
    22801 agtaggaggg ggccatggaa tataatgcaa tggaactcat gatatgtgtc gcagcacgca
    22861 atttagagga tggggcgaca gttgttgtag gaacaggtgc gccctgtgca gcagcaatgt
    22921 tagctcaaaa gctatattca ccaaatttat gtatcatgtt tgaggctggt ggcgttgcac
    22981 ccatattacc caccatgccc atttcggttg gagattcccg tacgtttcat aaagcaatta
    23041 tggcaagctc aatgccagag gtcatggaaa cctgccagcg tggtatggtt gactatacct
    23101 ttttgggcgg tgcacagata gatatgtatg gcaatataaa ttctacaatg attggcgatg
    23161 attatgaaaa acctaaggta cgctttcctg gttccggtgg tgctaatgat ttagcctcac
    23221 tgtgctggcg cacgatgatg atgacagtgc aggatgctcg caggtttact gataaattgg
    23281 attttatcac ttctccgggt tatctacagg gaggaagaag ccgctatgaa gccggtttgc
    23341 ctgaagggac aggtccatac cggattatca ccaatatggc agtgatgggt tttgatgagg
    23401 aaaccaagtg gatgacagtg ctttcaataa atccaggata cagccgtaaa gatgtgcagg
    23461 acaattgtgg atttgagtta aaatgggcaa agaagattga ggacaccaaa ccgccaactg
    23521 atgaagaact cagaatttta cgtgaagagg ttgatcctca ccggtatatc attggaagat
    23581 aaggagatag ttatggaaga tatctttaag ggaattgaac cacaggtata taaaagccag
    23641 ataagtgttc cctatcaatg gtgggcagga gaaacagcat ctaaattttt cataaccctt
    23701 cgtgatgaga agaaaatact ggggactgag tgccctcagt gtgcaaaagt gttctgtcct
    23761 ccgaagaaaa catgcccatt ttgctatgtg gatacaaaga actggaaaga ggttgggccc
    23821 aaagggaaag ttattacata tactgtagta tacaggcaac ttgcagcgtt aaagaaaaat
    23881 gtgcctgttg catatgggct catacagctt gagggttcag ccaacgcaat gttgcactat
    23941 attgaaggaa ttgaacctga aaagatttca aaaggaatgg aagtagaagc tgtttttgct
    24001 gatgaaaaaa gtgcttctat acatgccatt tcttacttca ggccggtaaa ataggaggtt
    24061 agcaatggaa gctgcaataa ttggtgttgg tatgaccaca atagaaaaaa gcaaggttgg
    24121 tgaaacgtat gctgacctgg cctgggaagc cgttaacaaa gcattgaatg atgccggttt
    24181 aggaattgat gatattgata atgtcattac cacttccagt gatttctggg acggcagaac
    24241 catatcgtgt atggcagtag gagatgccag tggggttaga tataaaaact gctctgctgt
    24301 tgaaggtgat ggaacatttg gggcagcata tgcacttgca cgctcacttt ccggtgatta
    24361 taaaacaaca ctggtaacgg cacatagtaa aggatctgag agtatctcca gcatcattac
    24421 caatgcggcg tttgatccta tatatgagcg tgggctagga atggatatga tttcggcatg
    24481 tgcaatgcag gcaagggcat atatgcatgc tactgggaca acaccggaac agcttgcact
    24541 ggtttcatgc aaaaatcaca aaaatgcaaa ggataatccc tatgcacagt tgccaatgga
    24601 tatcacggtg aaagatgtat taagttcaga aatgattgca gatccgctgc ataaattaga
    24661 ttgctcaccg gtttctgatg ggtgttgtgc aattatcata gctaatgccg attttgtgaa
    24721 aaagattaaa gctaagccag tatggattaa aggtgtagca ttctgcagtg atgcatattt
    24781 tatgggcgat agagatttat caaaagctaa agcactggaa gaagctgcaa agaaagcata
    24841 ttcaatggca ggtattacaa atccgcaaaa tgaaattaat gtggcagaaa tatatgatgc
    24901 atttacctat caggaactta tgtggattga aggactgggt ttggcagaat atggaaaggc
    24961 aggtttgatg cttgaaaaag gcatatttaa ccgggatggt aagctgccag taaatccatc
    25021 aggtggatta ttaagtggtc accccgtcat tgcagcaggt ttgtaccgaa ttgctgaggt
    25081 tgtgttgcaa ttgcgtggtg aagcaggtgg gcggcaggtt aacgcaaaac gtggtgttgc
    25141 ccatggtgtc aatggattat gtggacaatc ccattgcgta tgggttcttg ataaagagaa
    25201 ataggaggca attatgggaa agcgaagagt tgcaatagtt ggaaccggtc aaactcatca
    25261 ccgaagccac aggcccgatg ttaatgggcg ggaattaata catgaagcag taacccgtgc
    25321 tctggaggat gctgatcttt ccataaagaa tattgatgct gtaatcattg gcaacatgga
    25381 ccactttgaa ggtatcaatt acgttgattg ctggagtatc gatggttcag gtggatatca
    25441 taaaccggtt attaaattaa caactggtgg aacaacaggt agtactctgg caataggtgg
    25501 ataccatttt gtagcatcag gaatgtttga taaagtttta atcattggct gggaaaagaa
    25561 ttctgagtca gatactacag gagctatcac tactgcattt gaccccgtgt gggataggtt
    25621 ggtttttgca ggagctatct ccggacttgc cgttgaggcg caaagttaca tggcaaaata
    25681 tggagcaact gacagggatg gagcgcgggt atcagtgcgt gaccgcaagc atgcatgtaa
    25741 taatccttat gcacagctta gaaaagaggt tacactggaa gaggttttag catcaccaat
    25801 gcttgctgac cccattcatc ttcttgatgt gtgtccccgc accgatggtg catgtgcagt
    25861 gataatggca agcgaagatt atgcagaaaa aataacatca acacctgact ggatatgggc
    25921 aacagcaaac aggcataaat atacatattt aggcgatgta aactggggcg agcttgacag
    25981 tatggctgag gcctcaaagg atttgtttaa aaagtgtggt attactgaac cacgaaagca
    26041 gattgatgtt atagaaatgt atcagccata ttcgtttgcg ggtataatct ggattgagga
    26101 tatgggaata gtcccacgtg gcaaaggagc agaatatata tggagtggtg caacagatat
    26161 gggaggcgaa cttcctatta acccttccgg aggagtaatt gcatgtaatc ctattggtgc
    26221 tactggttta atacgctgtg cagaagctgc attgcaggtt atgggaaagg ctgaagcaag
    26281 gcaggtgcct gatgtcaact ttgctgtaag cacaggtttt ggtggctgta tgtggacaga
    26341 tatgcttttg catggtaaga agaaacccaa tctataggag gatacaatgg aaaacattga
    26401 atatataatc atagaatcgg ggaaagctgt acaaccgttt aattattcag ttggaatgta
    26461 tgggagtaaa ttttttcatg agttaaaaaa taacaggaaa tttgttggtg tacgctgtcc
    26521 aaaatgcaaa aaggtctatg tgccaccaag aaaagtgtgt ggtgactgct ttgttgaaat
    26581 gaaagaattt gtagaagttg gtccaaaagg tacaataggt acatttacta ttttacgatt
    26641 tgcatttatt gatcctgaaa ctggaacaca gaaacccata ccctacggct acggatttat
    26701 aaagttagat ggtgctgata cattatttca gcactatatt gatgttcacg atgaatcaaa
    26761 agtaaaaatt ggtgcacgag ttgagcccgt tttttctgat gaaaagaagg gtaacattaa
    26821 agacatcctg tatttcaggg tcattgacta actatgagta ttattgaatt acataatgat
    26881 gtgagggcgg tgatagatga aagagctttt gccatggtac ttatcaccgg tactgataaa
    26941 gtccgttctg gctatggtaa tggtgatagt ttattggtag attttgatca ccgagtattt
    27001 gcagttgctg atgcaacaga gcgcttccct cacgcatcac gcttactgct ttcaaatatt
    27061 gtaagtggtt tgcaaacaat tcctcaaagc agtgatgagt ggttacacta tctgaatgga
    27121 atttatgcca gtcaggaata tcatttaaaa tccacactga gtttgatagc acttcaaaaa
    27181 caggagtctg tttcacgggc atttattgca catggtggcg atagttcatt acaaatatat
    27241 aacaagcaga ataagagcct ggtgtacaaa acgtcggtga atatgaattt tgccggaaga
    27301 agcaagcacg ttgattcaat tgattgtata ccactgcaag atcagttaag agttattttg
    27361 tatagtgatg gtttccatga tgttatcaag cattgcaata ttgtaatgga agatttgctt
    27421 catgtttcat tgatagaagc tataacgcat attattgata aggttgggaa tttggactgt
    27481 gaagagtatg atgatataag ccttttgtta tttgatattc ctgaaatata tacggtaaat
    27541 acgaagggaa ttctgatggg tgggaccaca agactgcagg agcagaaagt caaagaatca
    27601 atcagtatcc atgattataa ttctttgatt aggataatgt aaaaatgatt tctgataata
    27661 ttgattggtc attcaatcat atggatggag attcaaatga aaattaagga acgctcagat
    27721 atttttgata tacgacgcgc acagcttacc tctgctactt acaatgtggt gagtaagaag
    27781 gggtattata atttcactat aaaggatatt gcccaggaag caggtatgtc aacagggttg
    27841 atccactatt attttaagaa taaacaggac ctgcttttaa atttactcag agagattaat
    27901 cgtaatatta gaacatcatt acagcatgat cttgaacacc tatcggaccc tgttgacaga
    27961 ttagctgctt ttatagaacg ggcatgcaat ctggtaatgc aggaaaagaa ttacttctat
    28021 gtgcttctgg atttctggac acagctgaac cataatgaac ggatgcgcac tgctattcag
    28081 aaactatata ctagctatcg tgatgtatgt tcggaaattt taaatgaagg gatagagcag
    28141 ggaaaattca ataacataga tatcaggtat accgctacca tgattgtttc catggttcag
    28201 ggtcttatta ttcaatatgt tatagatcct gatgcatttg attatgcgca ttatgcacat
    28261 aaaataaaaa atcaaataat tgaaactata cttaacaaag gaggtcaatg atgaaatttg
    28321 ggttcagtga ggagcagctt ttattcaggg atacaatata taaatatgca aaaaatgaaa
    28381 ttgttcctct gtgtgaagaa gctgatttaa aatcagagtt ttcatttgaa atctggaaaa
    28441 agttagggca aatgggtctt ttaggtttgc cattcccgga agaatatggt ggtcaggggg
    28501 ccgatgtggt aacatgttgc cttgctggtg aagcgttggg tcatgctggt gttgatagcg
    28561 ggcatttatt agcattaggt gcacacacgt atttatgtac tgatactcta tacaaacatg
    28621 gaaatgaagc acaacgtaaa aaatatatac caaaattagc cagtggtgaa tggattggat
    28681 gtatgggatt aacagaacct ggtgctggtt ctgatgctgc atcactacaa acaactgcag
    28741 ttaaaaaagg cgatagatgg atattgaacg gttcaaaaac attcattacc aatgccccgg
    28801 ttgcaaatgt atgcgttgta tatgccactg tggataaatc aaaaaaacat gctggaataa
    28861 cagcatttat cgtagaaaaa gattttaaag gtttttctac agggaagcca ttccataaaa
    28921 tgggtgtcag agcatcaact acctctgaag tgttctttga agattgtgaa gtccctgaag
    28981 aaaatgtact gggtaatatt ggtgaaggtt ttatgtatgc tcaccagacg ttggaatggg
    29041 accgcagcgc attgcttgca ccatttgttg gtggcatgca gttcatgata gaacagtgtg
    29101 cacggtatgc ttctgagcgt gtgcagtttg agaagccaat taaaagtttt caggcaattc
    29161 agcataaatt agctgatatg aaggtggtac ttgaagcagc cagactggca gtgtaccggg
    29221 ttgcgtcact gaaagatgct ggatacccaa ttaaccatct gcaggcatct attgctaaag
    29281 cttttgttgg tgactggggg tttaaggtta cttcagaagc tgtacagata tttggtgggt
    29341 atggatatat tcatgattat ccagtggagc gaatgctgcg cgatgcaaaa ttagctcaga
    29401 taggcggtgg cacttctgaa gtacaacgat tgattatatc ccgattgttg gcattataat
    29461 ctggtggcat attaggaggt aaaaaatgaa ttatgaattt aacgatatac aaaaatcaat
    29521 aaaggaaaac tttaaaaaat tttgtgaaaa tgaaataaaa ccacatgcag cattgcttga
    29581 tattggtgat gagaaatctg ttgagatttt gaagaagaat attaagaaac tgggtgattt
    29641 aggctattta ggcatgattc atgaggaaga atacggagga acaaatcagg atcttataaa
    29701 ttttgccatt gcaggtgaag aagttgcaaa agcctgtgct gctacatttc tatcagcagg
    29761 cgcaagctgt ggtttatgcg gtatgcctat acggcttttt ggatcaaaaa agcaaaagga
    29821 aaaatattta ccagggataa tatcgggtga catcattggc agctttgggt taactgagcc
    29881 gcaggcagga tcagacgcgg ttgctatcgc cacaactgca aaaaagaaag gcgataaatg
    29941 gattttaaac ggtaccaaaa catttatcac caatgctccc attgccgatg tgtgcctggt
    30001 catggcatat aatgataagg aggcaggacc tgctaatggt gttacagcat ttcttgttga
    30061 taaaggaaca cctggttttt caacaggaaa gccatttgaa aagttaggat accgtggttc
    30121 accaacatca gaagtattcc tggaggattg tgaagttggt gaagatgcag tgttaggcga
    30181 agtaggcaag ggctttattc aggcaatgca aacactggag tttggaagga ttggcatggc
    30241 aacagtttca ctgggtgttg caatggcatg catggaagag gctgtgaaat attcagctga
    30301 acgtaaagct tttggcaagc caataaacag gtatcaggaa atttcattta aactggctga
    30361 tatgatgata cttactgacc tttcacggct tttgatttat aaagctgcgt gggccaagca
    30421 aaccaataat cccgaagctg ccatattggc ttcatgtgca aaattatttg caagtgaagc
    30481 agctactcag atttctagct gggcactgca gatacatggt ggttatgggt atattaaaga
    30541 atttcctgtt gagcgcctat accgtgatgc aaagttgggt gagataggcg agggtacatc
    30601 tgaaattcag aggatcctca tagcccgtga cctgttacag aaatatggaa gttaatatga
    30661 ggaggtgaaa caatggctat tgacaaaaaa tatattggca aacaatggcc aacgatggtc
    30721 tatgaggttg gcaaagaaaa ggtaaaggaa tttgcaaaag ctattaaaag caccaacccg
    30781 tattttttag atgatgaatt tgctgaacaa tcaaagtata aagttattgt tgcccctcca
    30841 acatttgctg tagtgtttgg agctaaactc attgaaccaa tattttttga taatgagctt
    30901 aacctcaatt tagcaatgct tgtacatgga gagcaggagt ttgaatttta caatgttgta
    30961 aaatcgggtg atgtgctgta ttcagatgca aagatagtaa atattgagaa taaggaaaaa
    31021 ttagatgtaa ttgcaattga aatcatgacc agaaatcagg aaaagaaaga tgtatgtaaa
    31081 ggaatttata cttttgtggt gaggaaatag ttatgaaaac aatggattca cacaaaacaa
    31141 taagtgttgg cgatagtttc aaggtaaatc aggaagttga taaatacagg gctatctact
    31201 atgctggtgc atcgggtgac tttaatccca ttcatattga tgctgagttt ggtaaaatgg
    31261 tgggtcttgg tggtgccata ttgcagggtt tgtgcacact gggatttacc gctgatgcaa
    31321 ttaccacgtg ggtaagtgac cctggacggt tgaagaagtt gaaatgcaga tttgcatcgc
    31381 cagtgcgtat gggggatacg ctgactattg aaggaacagt atcagaagta caggaaaaca
    31441 gagcaaaact tactgttaca gtaaaaaatc aggatggcat tgaagtatta acaaaggttc
    31501 aggctgaaat agaggtatga tatatagggg ctgtcccaaa agaggtcgtt gagtaaattt
    31561 tgcaaacatt atatcgtaat actcgctcat ttcgatacaa ttttgccata aaatctggca
    31621 aaattactca atgagcgttg caaaatttgt atcgaaacgc catttgggac actcccgtgt
    31681 tggttggcct tatcattcaa caggcatgaa ataactatta tatattttta tcttcctatc
    31741 attgttaaat aataaaaaaa gtttttgttt catactattt gcatatggtg atgttttata
    31801 tatattgttt cgctcctcag ggtcctgtga tatatcatac aattcatatt ctatcccata
    31861 atgcgtaggc atgtagataa gttttagatt gtttgcaacg atgctacggt gttttgctat
    31921 ggtaatcaga ttgtcataat atggtgttac tgtgatctca tcatggtgat taaaatctat
    31981 ggttgatatg ccagttatat caggatacat tattcgtttt ttctgaaaga aaaaatttcc
    32041 agtattgtca aaccatatac cagtttcaca atatgcatca aatgtacggt tatggttttt
    32101 cataagggat gttccatcaa aggattgcgg ttgttgtata tttaaaagat caagaattgt
    32161 aggtgtaata tctattgcac ttctgacagc tgtgtatgcc tggtgcggga taccaggtcc
    32221 agaaaaaata cagggtatta tcaggctgta tttaccacga agatgttcac catgccccat
    32281 cccgtattca tgctcataaa ggttttcgcc atgatcggat acaataatga tgatggtatt
    32341 gtcgtaaagg tttttatctt tgagatactg cattatggcg cctacagctt catcaaatgc
    32401 atacagacac ccatcataga gtgcacgaat atattctatc tcctgtaatg aaggctcata
    32461 ttctgatgca acgccgctgc tatcaagaga aaccactcta ttttttgaat atttaaaagg
    32521 gcccttatat gaagtatcag taaaatagtt gtagtatggg taacaggcgg ggtatggaaa
    32581 atgcgtaatt gaaaaaaatg ttgtgatgaa aaatggcttt tttgtatgaa tacttttgct
    32641 gatttcagat ttaatgtcat caataacaaa ctgcgggtcg gcaaattcag caaattcgcg
    32701 aatttcagga aacagtgtaa atccaattct attagttaaa aatggcaaaa gaaatgtatg
    32761 gtttttaagt attatctgtt ttatcatgac acgcatgttg aatgttggcg ctttcacagt
    32821 gtcaaagccc aagttaatgc ggggaaaaat atcagcagca aaatcaccaa caacacttgt
    32881 ataatacccg taatctttta atactgttgc cagagtaaca aaatttctat ttcgtgattg
    32941 tgccgttggg aacatatggc caatcccgtg tttggccgga agttgtgagg ttaaaatact
    33001 tacccaggca ggaaacgttc gcggtactac cgtataaaca ttggtacagg tggttccctg
    33061 tgcaatcagt gtgtctatgt ttggtgttgt tttgcggtga taaccaaatc cgctgaaatg
    33121 gtcaggtcgc aatgcatcag aagctaaaat aataatatta ggcttttcat gtgtgttttt
    33181 atatgagata gttcctgtaa cagtaactat cagtacaatc acagctataa aaagagctgt
    33241 tgcaacaaat ttccatctat taaacaggat aatcagtata tcgatgatat cattcttgcg
    33301 ttttgttaag taagtaaata caggaagtag cagtgctact gaaaatggag caagaataca
    33361 tatttgtatt atttcaatta caacaggtgc aatgttattt gtaagagcta tctgaaaata
    33421 gttatataca atatttttgt tgaatatagt ttcctgatat aattgcggat ataatacaat
    33481 acttttacaa agctgaagaa aaataatagt aaatggtaag attgccgaag agtagaaggt
    33541 acgcttggaa tgcatagttt tattaaggaa agtcaaaatc tgccttatac cgtgattaaa
    33601 aaaaatactc aatataatcc ctaaacaagc gtaaacaact attattttta caaagtacag
    33661 tactatcaaa aatttgtaac gatagagaat aaaatgtaca acatcggtac tggtattgcc
    33721 catatatgaa aatgacatat taaatacgat ggcataaaga aacaaagcac cagatacaag
    33781 caatcctggt aagtatatat tttcatatga taatattttc tttttaccgt ttttgttcac
    33841 cataatcctc aagcaataga attgtatatg cttaaaacgt tttttaattg gtgcggttat
    33901 atatcaagta aaaaatgaaa cacgtataga tattcaatca gtgtgtaata tgcctatcag
    33961 gttctgcaat ttcaaggaaa ataatgaatt taagttaaaa tagcaaaata tgttgaaaat
    34021 attaacaatt ttgtgattat ggtgtcaaaa tagttctaga tgtaattann nnnnnnnnnn
    34081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34141 nnnnnnnnnn nnnnnnnnnn nnnnnnnntg aaaatattaa caattttgtg attatggtgt
    34201 caaaatagtt ctagatgtaa ttacaataaa atatattcaa ggaggtttgt cattatgggc
    34261 ggcaacactg tcttgattga aattaataat tttgtggcaa ctttaacgct taacagacca
    34321 ccaacaaatt caattaatat gtcaatgaga gtggagcttg atgaattact taatcaatgt
    34381 gaaaacaata atgacgtgcg ggttgtgatt attacaggtg ctggcgataa aggatttagc
    34441 gcgggcatgg atgtttcgga tgttacaaat ctacataacg gtccagatgc catagaatta
    34501 ttcaataaaa ttgatagata taaaaagcca gtaatagctg caataaatgg atatgcgctg
    34561 ggaggcggtt gtgaattagc tttagcttgt catttccgtt tcatgataga tgcgccaaag
    34621 gctgtgatag gatgccccga aataaatctt ggtataactc caggatgggg tggtatacaa
    34681 cgcatggcac gcctattagg cagaagcaaa gctcttgagt taattctttt aggtaaaaga
    34741 ctaaccgcaa aagaagctct ggaaattggg ctcattgata aaatattttc tgctgaaaca
    34801 tttcaccagg aaacaatgga atttgcaaat caattggcta aaatggcccc attagcagta
    34861 caaagtattc ttaaagggat gataacaggt attgaaaaag ggattgaaga aggattaaaa
    34921 gtggatagag aatgttcaga aatgcttgca aaaacagaag atgctgctga aggattcatg
    34981 gcatttgcac aaaaacgtga gcctgttttc aagggcaggt agttgattac ataaaaatta
    35041 tatttttata gtggtacata taccagctgg catgaacagc tggtatatgt ttattaatcg
    35101 caaaatattt ttaatagtta tcgcacaata gctcaaaata gctttgtgga tgcagacagg
    35161 ttggacattt ttcaggtgca gattcacttt catggatata gccacaattg cggcatttcc
    35221 actgtacttt tttatccttc ttgaatacct tgccactctg gatgtttttc attaaatcaa
    35281 gatagcgctg ttcatgatgc ttttcaactt ctgcaacctt tctaaaaaga aaagctattt
    35341 caggaaaacc ttcttcttca gcaatttttg cagcttcagg atacagtttt gtgtgctctt
    35401 cattttcgcc tgctgcggca gcttttaagt tttccacagt ggtgccaata acgccagcag
    35461 ggtaacttgc ctgtatctca accatgccac cttctagaaa gctgaagaaa cgtttggcat
    35521 gttctttttc attaagagca gtctcctgaa atatagcagc aatctgctca tagccttcct
    35581 ttttggcaac tgatgcaaaa tagtcatagc gtgtacgtgc ctgagattca ccggcaaaag
    35641 ctgccagtaa atttttttca gttcttgtgc cttttacact ttttgccata gtatatacct
    35701 ccctaagaaa ttataattat tctgaaccac gttttcccag tatcttatca tactgtaaaa
    35761 tgccgttaac agaatctttc accatcttta tacgctcaag tatgctgctt gcattggctt
    35821 cttcttctac ctgttcttta ataaaccagt gcagcataac ctcagtagca taatcctttt
    35881 cctgtcgggc tatatcagct aaattataaa taagcgacgt cacatgctgt tcatgtgcat
    35941 agacagcttc aaatacatct aatggagatt tccattcaga ttgtggtttt tgaattgcat
    36001 aacctatgcc ttccacaagc cgtgtaagtt gcaatattca cgtactgtga agctttcagc
    36061 atcaacttta aacagtgcaa caggcttttc acctggtttt aagaattgcc tgtaggcttt
    36121 ggggcccgca attagctgta tccattcaat gtaatgtttc tcttccattg gatgttcaac
    36181 gctgccaacc tgtaccttta tgccttcagc agttttttca acggctggta catgtttttc
    36241 ttttgaagca tcaacggtgt tttcaatttg cagcttcatg ggctgaccac agcacacaag
    36301 ttcgcctttg ccttcatgaa gcacttcaac aatattgcca caaatttcac atttatacac
    36361 ttcaagttgt tttgtagcca tgatttgatc ctccttgttt tataataaag tagttaaaat
    36421 attacgaaac ttttccttta aaaaccttgt aaacgtatat tgtgtatgcc agtactacag
    36481 gcataccaat aagagcaata atgaacataa tttttaatgt cagctgagtt gatgatgcat
    36541 tgaatatggt caggttatat gcttcaatgg ttgatctcac aagattggga tataatgcag
    36601 cacctgccat aatccacagt gatacaaatg aaactgatga gcccataaat gcattaaatg
    36661 atcggttatc tctgttacat acatacacgt aactcaaaga aattgcatat acaagtgccc
    36721 caagccagcc agataaattt gatacaattg aaggcaaaaa tccaatagta agagctatcg
    36781 ccagtataaa taagaaagca ttggcaataa ttagtttgtt cattaaagct tttgcacgtc
    36841 tggcaacatc gccttcaatt ttatatgctg cccaggtaca tccctgaaga agtattgccg
    36901 ataaccccaa aaggccaatg acaagtggaa atggccttag cagtgtaaaa aagctaccag
    36961 taaaattcat gtttaaatca attgggattc cgtagataat gtttcccagt gcaaccccaa
    37021 acagcaacga cggtaaaaag ctgccaatgg taaatgccca tgcccacagt ttcctgcgct
    37081 tttcatcata ataccagaat tccattgaaa cagccctgaa aatgagagca aatagcacca
    37141 gcatcaatgc caggtaaaat ccgctgaaaa cggtagcata cgcaaagggg aatgctgcaa
    37201 aaagtgcagc accgcctgta ataagccata cttcattgcc atcccacaca ggccatattg
    37261 aatccataac ctgcttttgt tctttttcat tttttgccag cgcaggcata aggcttgcta
    37321 tgcctaaatc aaacccatca agaattgagt aaacagttaa taaaaatcct ataataaaaa
    37381 accatatatt ctgatataca cttatatcca ttgtgaaacc tccagttgca ttaatatgat
    37441 tctgcctgag ggcctttctt tattattttt acaagtattg ttataaaaag aaccaccagt
    37501 aaaagataga taagtgaaaa tataatgagc gatagcaaca cgtgtgttgc cgggacaacg
    37561 gttgaaactg catcaacagt tttcagcacc ttatacacag cccatggctg gcggccaacc
    37621 tcagcagcaa tccagccaaa ttcattggca atgattggca ggggtatagc aaacatcagc
    37681 acatacaaaa accattgtgc agtaaaaagt tttttcttaa gtaaaaggaa taatgcaatc
    37741 aaactcatca acgcaaaaag tgaacctaac ccaatcataa catgatagga aaaataggga
    37801 agaagtacgg gtggaagttc atcttcaggg aacgcatcac gcccttttac ttcggcattg
    37861 ggatcaaagt aagcaagcag acttaataat tttgggattt taatttcaaa ataggtcttc
    37921 ttttcacttt caacaggtat tccaataata gacatgggtg caccctgttg tgtttgccac
    37981 agagcttcaa atgttgccat tttttcaggt tgcaggttag ttacctgtac tgcatgtaag
    38041 tgccctgagg caaactgcac cagtgacatg atgcaaaaga caataattgc agtttttaat
    38101 atattctgag cttcttttgt atgctgctgc ttaattatat accatgctgc aacccccgca
    38161 gcaaaaagac ttcctgaaat ccagccagca ataatcacat gaataaaccg taccagcgtt
    38221 gaaggattga gtgcagcagc ccagaaatca gttaatactg ctctgccacc ttcaatggca
    38281 aagccagcag gtgtttgcat ccatgaattt gcaatgataa tccacagacc agataagtgg
    38341 gaaccaaaga atactaaaaa tgctgaaagc aggtaaactt tttttgaaac tttattgcgt
    38401 gcaaaaagca gcacacctaa aaatacagac tcaaggaaga acgcaaagat accttcggct
    38461 gcaagtggtg caccaaaaat atcgccaacc attcgtgagt attcagccca gttggtgcca
    38521 aaagaaaatt ctaacacaat gccagtggcc acacccagtg caaacacaag ccctaaaatt
    38581 ttaataaaga aagtagatat tgttttgaat aactcttcgt ttgttttata aaatttatat
    38641 tcaaacagaa aaaccagtaa ggtaagccct aatgtaagtg aagggaagat gaaatgaacg
    38701 ccagcagcca atccaaattg aatacgtgat agcattacaa catccatgtg aaaacctcca
    38761 aattaattat ttgtacaata aaaaggatat gcagcgggtg catatccttt aattaatggt
    38821 atcaatcaac aggttcaaac tgatcttttt cagcaccaca cacggggcaa acccagtcat
    38881 caggtaaatc tttaaaagct gttccaggtt ttatcccgtt atcaggatca ccttctttgg
    38941 gatcataaat gtatccgcat accgtgcatt catatttttt atccatgtag tagacctcct
    39001 ataaatttat cttgtaataa actacaagcc ctcatgctaa ctgtgtggtg cttttaaaaa
    39061 aaagcaacat ggttatcctc acatgtaata gcatcaccat gttataacag ttcaggtgta
    39121 tagccagctt cttttatagc ttgcatcaca atagatgcat ctaaagagct atcgaccata
    39181 accattttgg tggttacatt acattgcact tttgcagcag gatctttttc gtttattgct
    39241 tttgtaatat gcattacgca gtgattgcac atcatgtcat taacacggaa ttgcattgtt
    39301 actcctttta tccatagttt tagtatggta cagtataaaa cataaattac aagtaaaatt
    39361 taaaataatc tatctcaaca tcgtctatca catatagata tgtttataga atattttgat
    39421 gctattcatc gttaataatt gtagcatata taatgtaatt atctcaaaat tataataatt
    39481 acaaaatttt gtcaagcaat ttataaaaaa agaattgtaa aaagatttca gtataactaa
    39541 aactttcagg aacaaaagat ttaatgtgat gagcactctt tgcagatgcc ttttaatgtg
    39601 atttgatact gttcaattgt atggccgtct attgattcag gcatgttgac gtaacgggta
    39661 atatccaggt catataccct tccgcaccgg gtgcaggtaa aatgaccatg tggctgaacc
    39721 gttatatcaa aacgcgcctc aacgttgtca agaaccagca tgcttgccag gccttcctga
    39781 acaaaaagac tcagtgtatt atatacagta gttttggaaa gagttggtat atgtggcaaa
    39841 agcgctgtat aaatctcatc aacagttggg tgcgttctgt tctggtctaa atactccaga
    39901 atcttcagtc gctgatatga tggcgaaatc cccttggctt ccaggtaatg tttatattgt
    39961 tccattgtgg tcctcattgt aataataaat tggactcatt atgcaattat ataatacata
    40021 atactgtagt aaaagtcatc ttaaatttat aatttttcaa aaacctgttg ggcgatagtt
    40081 cccagcaatg gttcatcagg tacatactgg accttaatgg aaggaagcag ttgccatccc
    40141 atttccgcta ataccgcttc aacctgtgca acgctttgtc cgccccagcc gtatgatcca
    40201 aatgcaagcc ccacgcgatt ctttggtgca aggcccttgc agtaggttaa aaatgcagca
    40261 acagttggca gcatattgtt gttaagtgta ggagagccaa cacagatata ctttgcatca
    40321 agtacttcgg tcatgatatc agaaatatgg atggttttta aatcataaag ctgtgcagga
    40381 atattttttt cctcaaaggc tttttttatt gcatgtgcaa tgagtttagt tgaattccac
    40441 atggtgtcat aaatgattaa cgctttttct tttgttttat tggcagccca gttgttatac
    40501 tcagtaacta tcgcccttac atgatctttc catataatcc catggctggg tgcaatcatg
    40561 gcaatatcca gtgtgcttac cgcatcaagc actttctgag tttgtttatc atacggcaat
    40621 acaatgttag catagtattt ttttgcctca tacatgatga tgtccagcgg atattctgag
    40681 tcaaagcgtt ctgatgttgc tatatgctgt ccaaaggcat cgttggaaaa tagaatttta
    40741 gtttcaggca tataacaggc catgttgtca ggccagtgta ccatctgttc aaggatgaat
    40801 tgcagcgtgg tttttcctaa cgagatacta tcgcccgtct tcatgacttt gtaattccag
    40861 ttctttttgt aatgagcctc taaccccttt gcgccattgg gtgatgtgat gagcgttgcg
    40921 ttgggacaca actccagaag ttttggcagc ccgcctgaat ggtccatttc aacatgatta
    40981 gaaataataa tgtcaatctt tgcaggatca accactttgg aaatgcgttc aatcatttca
    41041 tcatacagat agtatttcac agtatcaata agggtaatct tttcatccat gataagatac
    41101 gcgttatacg tagacccgcg ttgtgtcata taaccgtgga agttgcgcaa atcccagtca
    41161 atgccgccaa cccagtatat aaagtcattc aatgcaacag gtttcatgtt gtcctccgct
    41221 aaagtggttt attttcttta ctcatcattg ttacagttca ggtgccaaaa ataaaatttg
    41281 catttaatat taaatataac ttcaatttaa ttttgttcaa gaataaaatt gtaattattt
    41341 tatttttgta cttttattgt gcgggaatta ctggaacacc agcttttaaa gctgctgcct
    41401 taagttctgc atcgaatgtt gccagagttg ctttttttcg taaggataaa tctaaataaa
    41461 cagcatcata ggctgatatt gaatacaaat ttgataaatt aaaaatattt gtagaaaata
    41521 gcaatccata ttcagtgtcg gtttcgagct tcaaagcatt caaaagattt aaaatttctt
    41581 gtgcttgaag tggtgttatt cttttacgtt taactgctat gtagataaca ttgcatgttt
    41641 cgtaccacca gagtacaggt accaataggc tttttttatt aatgtacttt ttaaaaaaat
    41701 tagttgatat tgatgaattt tcatctggca agaaaagtga agaagaaaaa gaacaatcaa
    41761 taacacacgt cattaatatt ttcttccttc tttaatatat tcttttatat ttacactttt
    41821 attaatagat gttcgtatgt tttcaaattt gtggaatatt tcttcaatat tgatattttc
    41881 gcttttggta tatgggacaa ttttggccac aggtttaccc cgttttgtta ttacgtatac
    41941 ttcacctttt tcaacattac tgagtatttt tgaaaggtgc gtctttgcat caaatgaacc
    42001 tattgttttg gttttcatat aactagtata ataaactagt tgtataaagt caattaaatt
    42061 ttcttgtaat tttttatttt cattgctgct tttggacaca caggtttgca ccaggtataa
    42121 agttgtgcac tgtagagcaa ttcttttgat tggtgcatgg cttccaatga ttcattgttt
    42181 aattgtgggt taaaggttgt aaatggcttt ttgggtgtat tgagaacagg tttttgtgcc
    42241 cccctgtaca atgttttgta cgatagtgat agtaattgtg tttttgcagt atcatctaaa
    42301 tgttttcctg tcattgcaaa atactcttct tcacattccc tggtaaactg atatacaggt
    42361 tccagtgcat aaaaatcctt gtcctgcttt tcagtaacaa atatatatgt aaccacaagt
    42421 attaatgaaa ttacaagaat caacactaag agggcaatgc gtagttgatg tatcataatt
    42481 attttatgtt cccttataaa ctgtgtgtat cataataaaa ttggagctaa agtcacaaaa
    42541 tggctgctat atatttatat tgcaataaga aattaatttt ttagggtact gcgagtaggt
    42601 attgtaaaaa aatttaatgc aaggacatta tatatagtta ttcatagtgc tatgtgttag
    42661 gttgtgaata acatacttat tgagataaaa tggtatatat gaaataaact ttttttacat
    42721 tgacatttaa aaaatagaat agtaattttg tatgaatgct aattgtaaca tacatggaat
    42781 tttatgttat aaatgatatt ttaaattaaa taatatagga acattatgaa agcaattagg
    42841 aaaattgtaa aacctgtaaa taataagata attattactt taaaagataa caatgaagct
    42901 gatgaatatg aagttattat tttgcctgtt gaaaacaaaa ataaccaaaa gataaaaagt
    42961 aagaaaaagt ttttagattt agcaggtaaa attaacattg atagtaaaac aattagtaaa
    43021 ttgagagaaa aaagcatatt atgattcttg ttgatacaag cattatcgtt gaatactgga
    43081 aaaatcctaa aagagagtat acagacgtat ttattacaca ggatattgct atatgtggca
    43141 ttgtacaggc cgaattgata tatggtgcac gttctaataa agaggttgaa aaaattattt
    43201 ctgcacttga atgtttcaat tttatagata tagataagga agattggaat ggcattggta
    43261 aatgtttaca taaattaaga ataaagggtg taaaaatacc atttcaggat gctataattg
    43321 catatttagc tgtaaaaaat aatataccct tatggacacg tgataaacat tttgaattga
    43381 tacgtgatgt tataaaagaa ttaaaagtat acaagccaat ctaatatcca caccattttt
    43441 cataatgcat tattgtatgt aacacctttt gatgatagaa tgcagtataa ttgtcatttg
    43501 ggtcatttgc gggttgaata attctttgta aaaaaaaatt aatgcaggga cattatatgt
    43561 cacacctcta ttttatattt gctacaaata tataatggag gtttgagtat gaatatttta
    43621 aacaaattgt atttaatcgg tatagtattc attgtgggct gtggcagttt gggtgtaagg
    43681 accgatgcac atagttataa agacccgctt tatggaacaa aactcatcaa aaagattgtt
    43741 gtttatgcaa acatacaaaa tatgcaatac cgtgatcaaa tagaatcagc atttattgat
    43801 tattttataa gcttgaacta tgattgcatt gctattaaga gttcaagtat aatacttcca
    43861 acacgaacat ataccaacaa agagctaaaa gagattttta aatcaaattc cattgatgca
    43921 ctattggtgt ttactgttca agatgcagga tacaaccatc aacaatttac atataatcaa
    43981 ccatatcaaa cgtatgggaa tgtttataaa agtggtagta attcatatag ctataacgca
    44041 tatacctatg gtggtccaca aacatataat ttttataaac cataccttag tgctgttgca
    44101 gacctgtacg atgttaacac tgaattaaaa atatggacgg ttcagttaaa tagccgtgga
    44161 aatgcatttg caaatattga tgatgcactt tataaatcga ttaaaacatc agtgttaaaa
    44221 ttggaacagg acggtataat aaaaagtcaa aaaaataccc agggttatta atacaacaaa
    44281 atgcttgaca ggagggaatt tttatttgta taagatggca aacaaaaatg aaatatcatc
    44341 aataaataac aggttacgac ctcgtggcat acattccata tcaataccac cgccattcca
    44401 tccgtttaaa agattacgat tattcacggc caggtgcata ttacatcacc atctgtacac
    44461 aaaaccgtgc gtgtttgttt gggaaggtgg ttgatggggt aatggaatta aatgatgcgg
    44521 gcaaaatgat tgaaaaatgg tattttgaaa tcatgaataa atttcccgat atccattgtg
    44581 accaacacat tattatgccc aaccacattc atttcattat cgttaatgtc ggccctgtag
    44641 gggcagacct gtgtgtctgc ccagaatcag ataaagaaat tattaatacg aatgataaac
    44701 catcaggtag atgttataaa aataatgata tacatgatga taatagaaat ttggtggaac
    44761 acaatattaa tattggagga tgcagaataa caaaacattg ggataaccgt aatggattgt
    44821 acgaacatgg gatacataat gatttgggtg tatatggaat aaaaaatgat ttgggcaaac
    44881 ataaaataaa aaacaatttg ggcggatatg ggataaaaaa tgatttgggt gaacataaaa
    44941 taaataataa ttttgacgga tatggaataa aaaacaattt gggtgaacat aaaataaaaa
    45001 acaatttggg cgaccataaa ataaaaaatg atttgaacgg acataaaata ataaacaatt
    45061 tgggcggata tggaataaaa aatgatttgg gcgaacataa tataaaaaac aatttgggcg
    45121 aacacgtagg ttcgccccta cacagggttg tacaatggta taaaacaatg acgacgaatg
    45181 aatatatacg tggtgttaaa caaaataaat ggcctcgatt tgatggcaaa ttatggcaac
    45241 gtaattatta tgaacatatt gttcgcaatc aaatggaatt aaaacgaatc agggaat
//
LOCUS       DAORYL010000014        42636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_726_length_42636_cov_12.347408, whole genome shotgun sequence.
ACCESSION   DAORYL010000014 DAORYL010000000
VERSION     DAORYL010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 42636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42636
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_726_length_42636_cov_12.347408"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="PLV81_04530"
     rRNA            complement(<1..819)
                     /locus_tag="PLV81_04530"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(1330..2553)
                     /locus_tag="PLV81_04535"
     CDS             complement(1330..2553)
                     /locus_tag="PLV81_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOM09400.1"
                     /translation="MTQWWEKSIIYNLDYLANNGILFKNAFVTTSLCSPSRASFLTGK
                     YASRHGVINNFSPWKAENVTFMELLKQGGYDTAFIGKWHMPGKGLPHLRGVDLFISFT
                     RKDGQGDYYNCPLFVNHKPVSNNKEYITEELTDYALEFISQPRKNPFCLYLSHKAVHH
                     DWKPPRHLKGLYKNEKIDFLAPESDKWNTWSGNNWLEGSMGSMHDIYKRYCECLVSVD
                     EQIGRIIQLLNKMGILENTVIVYAGDNGYMWGEHRLYAKHWPYEESIRIPFIFFYPGI
                     VKKPGKREQMILNIDLAPSLLQLAGIRIPDWMQGESFVPIVKNPLSPGRKSFMYELFK
                     DFPFGGRVPPNKTLRTERYKYIEWQACRGPELYDLLEDPKEMNNMIGTQKAKKIVPML
                     KKELEEIKIKYGAID"
     gene            2659..3066
                     /locus_tag="PLV81_04540"
     CDS             2659..3066
                     /locus_tag="PLV81_04540"
                     /inference="COORDINATES: protein motif:HMM:NF018391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HOM09401.1"
                     /translation="MISFDLECNNGHKFEGFFKDYNAFDDQMKAGLVECPVCNSREIK
                     RLYTGCSIQAKSSLLEKKQPTFFEMVRMVESYIKEHFENVGDRFPEVARAIYYGIEEE
                     RNIYGNASKEEMKELIDEGIPVLPLPKIDDIEN"
     gene            complement(3071..3553)
                     /locus_tag="PLV81_04545"
     CDS             complement(3071..3553)
                     /locus_tag="PLV81_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09402.1"
                     /translation="MLDFILDFIAKLLSYSMKFINWVADILMRLIDYCIGIFRELEIQ
                     EKIIILLSIVSVIIPILPIARFYIFESWYYVNNPLAVYFIGIIIIMVISTIVQKPWIL
                     ITRLIIIVYYLAWMIYLPAGNLITRAKPYELAYGYYINIAISIVYIGLCVFSLLSQRR
                     "
     gene            3661..3733
                     /locus_tag="PLV81_04550"
     tRNA            3661..3733
                     /locus_tag="PLV81_04550"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3694..3696,aa:Ala,seq:cgc)
     gene            complement(3745..6006)
                     /locus_tag="PLV81_04555"
     CDS             complement(3745..6006)
                     /locus_tag="PLV81_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOM09403.1"
                     /translation="MTRKTLLLITGGAVAIILLAIGIYRFYFLPVSYQDTITEGVAFP
                     VAIYRLDKGYPLIVAENRDDAIFALGIVHAQDRLLQMEFLRSLATATGVQDDLSQYES
                     IDRLVKAVGLPLKAKTLLSELSPQYLQTLQKYVDGINTYTFKMGHASKNKTHWSVEDI
                     VCITLFLEFCNAFVTNSELFFPVEDKDFPLLYRELANKKIVHSYRKQDFTIIKEIQKL
                     QEVVASIFGLYGSYTRGFAVAIPSRLMYTDSATLCSSYEHSVSLYPMIYPVNFQVAGK
                     VIEGYTCAGLPYVVAGKTNTLRYASFSLFADSQVFCKYQTQTRNNVLYYNSAAGYKEL
                     QCQPDASQCVSRFTDSGPVISDAFEYNLKDAIVAISYVSPAKGYIESMIDVAFANSIQ
                     DVIQIVQKYKGQPRVFIVQDDTRSYQIIAGSAMLDNESVLLSPEKALLLKNAPALFEI
                     KQADMCMAGSEFVENPSPAIQRVMVFNNTGRLSSLSHELSHYNEMQYKDIEKILYSVT
                     YPDAKEYLVIINNLLDKMPITSAKLAKVYFRDWDLTMGSSEVAPTLFNAIIAAMIRET
                     FADELPNAVKNMLTHYSIFEPEFKRIFKEDRSVYFDDITTLEKIESRDMIFDRAFLHS
                     LKPFNYTIGPYIDEWKWGKVHSATIHYLDSGSLVKNVMGKKKTIAFNGCASTVYRSLS
                     EQKNFSVDTVTVSCFYMDKMVHRASLSTSISSNPASHYYNEFVAKDSFKDINLSKRNE
                     LFLIKPVKEIQKQ"
     gene            complement(6022..7254)
                     /locus_tag="PLV81_04560"
     CDS             complement(6022..7254)
                     /locus_tag="PLV81_04560"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013431938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOM09404.1"
                     /translation="MESYIQELFADRIGGSQFGKVEKIYKFEKIKMAKREALKNNPGK
                     ELIDLGVGEPDEKAFDIVIESLCKEAYKHENRGYADNGIPEFKYAAAKYLKEVFGVEG
                     IDPETEVNHSIGSKPALAMLPSAFINPGDVCLMTVPGYPVFGTHTEWYGGQVYNIPLL
                     KNNNFLPDLDSIPADIVKKAKVLVINYPNNPTGAVATEDFYKKVIEFALKNKIMVVQD
                     AAYAALTYNGKPLSFLSIPGAKEVGVEIHSLSKAFNMTGWRMAFVAGNPLVVKAFANV
                     KDNYDSGQFKAIQKAAITALEHPELTEKIKEKYKRRLSSLVTTLRRLGFDAHMPGGTF
                     YLYVQAPKKTKSGIVFDNAESFSQYLIKECLISSVPWDDVGNFVRFSATFEAKDQNDE
                     QRILKEVETRLGGLAFEF"
     gene            complement(7283..7735)
                     /locus_tag="PLV81_04565"
     CDS             complement(7283..7735)
                     /locus_tag="PLV81_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09405.1"
                     /translation="MKINKYIILALLLTTGCTSMKFWIPSYSFDEVIAIKAQIDAAEN
                     PAKGFLLLKQLERKRVTVNNAIVKQISNSINIDYTFSVIATVEHSSGPIDCYIYTRNW
                     RNEEDFTSVARLKSGDTIYVIGRFSRFLKLPGNKYAVELVESNISKLK"
     gene            complement(7903..9006)
                     /locus_tag="PLV81_04570"
     CDS             complement(7903..9006)
                     /locus_tag="PLV81_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOM09406.1"
                     /translation="MIPRDSYKIVVQSKRLTTIFWVIFIGLIAGIAYIYRYYFWPFLF
                     ALIFYIGLKPLYTWILQYVKSRMLASFAMVGLIIVAILIPTFILLISLSDQVMEFYNF
                     LQLQFDPKVFKQYLMHSKFLKETLKYFNITQDELFQRIVRYMQDMSLTLFSNLTGLLT
                     FSIRFSINFFFMLLILFFLFKDSERFEKSIYTILPFPNDIEHDMVDTLKMVVRILLAG
                     NFMIMMLQGLFVGIGFVIVGLSTPVLWGSIAAVFSLIPVIGTSFVWLPASLYLLAIGS
                     PVKALIIALWCLGWYLLLENVLKPVVFGEKLNFHPLILFFLLLGSLQTFGLPGIIIGP
                     ILLTLFFSFWEMYKILDSYEAVSDNSAKTQKKK"
     gene            complement(9112..9300)
                     /locus_tag="PLV81_04575"
     CDS             complement(9112..9300)
                     /locus_tag="PLV81_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09407.1"
                     /translation="MTKKRGNDMKRLMNKEDREIKKKIAKEVSPKILEYRKKISDEAY
                     LEHAINRIATDLSHYLTK"
     gene            9600..10526
                     /locus_tag="PLV81_04580"
     CDS             9600..10526
                     /locus_tag="PLV81_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09408.1"
                     /translation="MNTIITKFIIFQLCIWLPITAGAYFSSKQYIDVQKLSQRLITIN
                     LISLESFIVLWTIWGLDLTPDLIILPFAGFTEVILGLLAGIAICTLTQCKGIQKHTFI
                     ISSSLANHGFTMGGFLCYLFIGEKGLALAFIFIIYFMPYVIMVIFSYARYVGTNAPFS
                     FKRYILTPQNMPLYAVFAALLLHYFDIKRPIINIPLDPMLATSLTIYYFSMGIYFKFK
                     DIKGFWKAHIMLALIKFIIVPLFMYFLLLFPQLSHDIKTVIQLQSFMPAAIYSVITSI
                     LFNLDKEMATSMFVVNTIVFSGIMMPLLIAIF"
     gene            complement(10598..10735)
                     /locus_tag="PLV81_04585"
     CDS             complement(10598..10735)
                     /locus_tag="PLV81_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09409.1"
                     /translation="MIRIIFCQTENKYIAMSLKNISGYSIFILSGFISGTIAHEDTII
                     V"
     gene            10859..12667
                     /locus_tag="PLV81_04590"
     CDS             10859..12667
                     /locus_tag="PLV81_04590"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOM09410.1"
                     /translation="MNTRLHHTIIIEYLGKIAASIFIFYFFIQYIHAAPPSQKGIFDL
                     RNYQWENGPVALDGEWEFYYGKFYDPKHFKNLKNINRQYMKIPASWSKYNINNQSLPA
                     SGFATYRATVLLSNNKPHSLSILIPKLDSSYSLYVNDILLVEVGKPGTTPDNSIHAWK
                     QTIADIPLTVNQIDITIHISNYYYAKAGITNSILLGNKQSILQHYIKSIIVKFFIFGA
                     LLFISLYHLALYIFKKNNKQSLYFGIFSLLMALLSIIFGEFHIMLIFPTLPWNILVRI
                     TYLTMSLGLTFFGLFIENLYPDYFNPSLFKSFLTIGILYTVLIIISPVFYFAKLLSLF
                     LGIMMVYGLLILFIFARAIKNNRKYAVFFLSAFLIFALAIVNDMLHFLHLINTTYMVP
                     YGFLIFIVFQSFILANQNSRLYSKLEKLFSEKIKLENIAATFQNLAYIDPLTELPNRR
                     RFEEYLHAEWHRAIRNQTPLSILMIDIDYFKKYNDKFGHIVGDLVLKKVGGTIKSCIH
                     RPADMCARYGGEEFIVILPETEIIGAYRIAERIRKKVLDLKLPAAITTSSKFVSVSIG
                     CTTEFPNLGDHWDYIIDKADKKLYQAKKLGRNRTEK"
     gene            complement(12720..13634)
                     /locus_tag="PLV81_04595"
     CDS             complement(12720..13634)
                     /locus_tag="PLV81_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09411.1"
                     /translation="MSLTIFDDIYNEIRKRNTAGGFKGVPYSDEFVKYVSGYYGLSID
                     LIKRIIQILINAHKIFSIEIIAEDKVRDISRVEGYVVADLVVIRKLKSFYQSELIIQY
                     ENEFHKRLMVHQIVKEIFPMLRSLNNTDIGKVANLAIMLEELERYMEKHYDEFTEEWK
                     AKRLAYEISISNIDLQLERKQSQKGKDDGQGSPSVKDRRKKRVVDAPQYKEFIDKSKS
                     YPLERIFQIYGIEFFLRVQLRNYKFDYLKELVQAKKIKRRSDLLLLKNMLNAVIHNRD
                     KDQQLVQYEDAIYSLMQTVNHHLFVERE"
     gene            13805..13879
                     /locus_tag="PLV81_04600"
     tRNA            13805..13879
                     /locus_tag="PLV81_04600"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13840..13842,aa:Asp,seq:gtc)
     gene            complement(13911..14900)
                     /gene="moaA"
                     /locus_tag="PLV81_04605"
     CDS             complement(13911..14900)
                     /gene="moaA"
                     /locus_tag="PLV81_04605"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HOM09412.1"
                     /translation="MLVDSFGRTIDYVRISVTDHCNLRCVYCMPADIKWLPHEEILRN
                     EEIVRLVDVFVSLGIKKVRFTGGEPLLRKGFFDIVKQVRSNHKELEICITTNGILLEQ
                     YIDKIQEFNVQKVNVSLDSLNPDTFAKITGVNAIHKVINGILKITALEGVECKINAVI
                     MESTLDELEELVRFATNIHAIRFIERMPLSNDPVPFVSADTLLQKLESLGTVQRKTTS
                     DTHVAAMYRFLPRWDKRAVINVGIIPAVSHRFCGKCNRLRVTSDGMLRACLHDATEYN
                     LKKILRGQLNDNIREVITNAVLHKKEGHSLMQCDDTAFNCSGMAMHSMSKIGG"
     gene            complement(14901..15461)
                     /locus_tag="PLV81_04610"
     CDS             complement(14901..15461)
                     /locus_tag="PLV81_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09413.1"
                     /translation="MNYRTVLLIIFVILCAHTSFAQQPFTFVKTDEPVSLWKALAWDV
                     LPGGGHIYTGHYEYAAVFAGLKISGVYAMYYYYNYWQYRGSLYRSAKKANEAIDPEHD
                     LLFKDPEGGYKTVKEFGYAYDRAAHYFTLSILANAAIYITSWLCTWYHVQEINESRQP
                     TYVGFNQFSFTTDENGTMWASCTMRW"
     gene            complement(15559..16053)
                     /locus_tag="PLV81_04615"
     CDS             complement(15559..16053)
                     /locus_tag="PLV81_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09414.1"
                     /translation="MSLNNKTIVLLLAIVLATGCLHEPVDEEYFDDLSRVKGGQKPTP
                     PVDLSMTINNYIITLSFYTDKIDNGQLLTYDPDTGSNENLYYLVYISATDPAQFSDPK
                     LYYDIKYYVGYVAETDFEEGVDPKVVTIQISSSYHGPVYVWMTSHDGGRESDHSEVVN
                     GDIP"
     gene            complement(16037..16468)
                     /locus_tag="PLV81_04620"
     CDS             complement(16037..16468)
                     /locus_tag="PLV81_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09415.1"
                     /translation="MWHRLLRIVLHGSVAFCLTACGYQYETIRCIRTVTVPHNDSQAI
                     SVQVNFTAYVAYIHNSSDIALTNNDAISRLAKGDVEGALTLLQPAIFTGEPAVLNNYG
                     IALILKGDFDKGYEYIYKAAVLKPHNPYFRKNYLYLHELKQ"
     gene            complement(16431..17858)
                     /gene="gatA"
                     /locus_tag="PLV81_04625"
     CDS             complement(16431..17858)
                     /gene="gatA"
                     /locus_tag="PLV81_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOM09416.1"
                     /translation="MAVKTVSQLVQMLERGETTSVEIVTEYLKAIDEHKDLNAYITVT
                     HDVALQQAQESDMRRKQGKPKSKYDGIPIAIKDNICTKGVLTTCASGILQNFVPPYNA
                     TAYQKLLDAGFVLLGKTNLDEFAMGSTTETSFFGPTRNPVDTTKVPGGSSGGSAAAVA
                     ADSVPVALGSDTGGSIRQPASFCGVVGIKPTYGRVSRYGLVAFASSLDQIGTFAHTVK
                     DAADVLAVISGIDERDATSINKPVDFDSTTITSNVRGLRIGVPQEYFNGVDEAIVTAV
                     KDVLKKLELQGAIIQEISLQYTDYAVPIYYLIATAEASSNLARYDGVRYGYRSKNIQK
                     LKDLYVKTRQEGFGKEVKRRIILGTFALSSGYYDAYYLKALKGRRLIIDDFTKAFSQC
                     DCIVAPVTTTTAFGIGEKISDPLSLYMADILTIQANLAGIPGMSVPVAKDAQGLPIGV
                     QIMAQHFNERVMLNVAQAIEDCVTR"
     gene            18183..20141
                     /locus_tag="PLV81_04630"
     CDS             18183..20141
                     /locus_tag="PLV81_04630"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HOM09417.1"
                     /translation="MKKHIVLYLLVSILFFGCYKKSVVLNPNDIHYIISTFLNEHVLY
                     HEFDDTISQRTLNNLLSSIDPGKYYFYKSDVDGFTKRYENLIDDLAKKEDFSFLDDIF
                     GTYKKRVDEVNHIIDGLLKQHYTFTEDETINLDSDKIPYAKDKNELRERWRKNIKLQL
                     LNYQTSESLNEDQAKEKLKRKFYLNNKRIQEFDKAKQFSVFLNAFSMALDPHTNYLTQ
                     EEHEDFMISNNLKLEGIGVQLRSEDGYTIVDRIIPGGAADKLPKELQLQPNDRIVAVA
                     QGNDEPVDVVDMELRDVVKMIRGKKGTLVKLTIIRINPDSKEQKRMIVPIVREEINLE
                     DSAVKSEVFQPENGPAIGYIKIPSFYVDMSQQGFGEGRSSTADTLMQINSLMKKNVAC
                     IVVDLRGNPGGALSEALKLAGIFIDAGPVMQVYDSSGNIQPLDDPIPGVYYSGPLVVL
                     IDKFSASASEIFAGAIRDYRRGLIIGTDATFGKGTVQSYKELYQKKGAIKITNAIFYQ
                     PSGTSNQLNGIKPDITVPDITTIWDIGENKLKYALQWKQIPPAAYTPYRNYLTADVIA
                     QLKVRSDNRLQNDAQYVKLLQEIKDLKQKLQSKEINLKDESSIEQRKKDLESSIKKGK
                     KDKLIDLENDVFLQEAFRITADYLKALH"
     gene            complement(20145..21212)
                     /locus_tag="PLV81_04635"
     CDS             complement(20145..21212)
                     /locus_tag="PLV81_04635"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOM09418.1"
                     /translation="MDKSTVIETIQQELDNLFARFSEIKQGGKKIIFTQCDSVPYEVF
                     VAHGFIVSKLPRWVMTALCKPDSSMYKRAVEYCSLADVLIVPQYCTIIPREVLQQLTV
                     HAIQHYEGFAQDAVVNLHTILYLVLQFLGVEQEYPDDDSLKQAVAVYEEIRKIMRALT
                     TMYAAVFTQSQLQLLCDAAFSLVPQQSLQLLQQLYHAVESSTHSDNEPTQKALVYSDF
                     SNWDIYDACNEFDIVIVEDDSCNGRRQFDISCHTSSQNLYYELLYAYSFKSWCPGMRT
                     VHERCELIYKALPNYDIKLVIFIQSFLNSRNEHLQSLYEQCLLQGTDAVILQADAITR
                     QLSHYSTALKARHHFDITVDL"
     gene            21345..22304
                     /locus_tag="PLV81_04640"
     CDS             21345..22304
                     /locus_tag="PLV81_04640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOM09419.1"
                     /translation="MKAHRANKPYLSIILPVYNEEENIVPQYQHIVEAMQKTSYTYEI
                     IFVDDGSTDNSFKLLESIAKKDKHVHVVQFRRNFGQTAAMAAGIDYSKGDIIIFMDSD
                     LQNDANDIPLLVQKIEEGYDVVSGWRKNRQDKLLSRKIPSRIANWLIAKVTGVYLHDL
                     GCSLKAYRGEIIRQVNLYGEMHRFIPVHASWIGASIVEVPVHHHPRKFGKSKYGIKRT
                     FKVLIDLATVKFMGSYSTKPNYIFGGIGMILLFISILSGITVIAMKLFLHHSMIRNPL
                     LLLTVMLIILGIMFILLGILAEIMIRIYHESQKLPPYRVKRYI"
     gene            complement(22347..23975)
                     /gene="groL"
                     /locus_tag="PLV81_04645"
     CDS             complement(22347..23975)
                     /gene="groL"
                     /locus_tag="PLV81_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805151.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOM09420.1"
                     /translation="MAKILQYNDEARRSIYQGVIKLSDAVKVTLGPRGRNVVIDKKFG
                     SPTITKDGVTVAKEIELEDPFENMGAQLVKEVATKTNDVAGDGTTTATILAAAIYHES
                     LKNVTAGANPMSLKRGIDKAVEAAVKFIGDTAREIKDKKEIAQVAAISANNDMEIGEL
                     IAEAMDKVGKDGVITVEEAKSIETHLEVVEGMQFDRGYISPYMVTDPENMEAVLEDAF
                     ILINEKKISTMKDLLPILEKVAQAGRPLLIIAEDVEGEALATIVVNTLRKVIQCVAVK
                     APGFGDRRKAMLEDIAILTKGQVISEDLGLKLENATLDMLGRAKKVIVDKENTTIIEG
                     AGSQADVQARIKVIKKQIEETTSDYDREKLQERLAKLAGGVAVINVGAATEVELKEKK
                     ARVEDALSATRAAVEEGIVPGGGLTLLYAQKAVAELEKKLEGDEKIGARIVAKAMEEP
                     MKMIAFNAGVEGSVIVEKAKQEKQGVGFNAATMEWEDLMKAGIIDPAKVVRSALQNAA
                     SVAGMLATTEVLITEKKEEEKAGMPGGMPPGGMY"
     gene            complement(24009..24293)
                     /gene="groES"
                     /locus_tag="PLV81_04650"
     CDS             complement(24009..24293)
                     /gene="groES"
                     /locus_tag="PLV81_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOM09421.1"
                     /translation="MGIKPLGDRVLIEVMEEELPKQGSLYIPDTAKEKPQQGKVLAVG
                     KGRYEDGKWIPLDVKVGDTVLFGKYAGTEVKYGGKEYLIVRENDILAIFE"
     gene            complement(24585..25739)
                     /locus_tag="PLV81_04655"
     CDS             complement(24585..25739)
                     /locus_tag="PLV81_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOM09422.1"
                     /translation="MREVIDQLELTYQRIKLFIPERKRPFFRTLDTENIRSFLLYGPR
                     GVGKTTFLIDTIKDKNFLYLSADSLAIHAYPLYDIIKEIFARGYDGVVVDEIHFSNNW
                     SMHIKNVYDDYPDKYIWISDSSNLILQKGMGDLSRRFVQYRMPMLSFREYVYLTEGIY
                     YDPVDVFSNYEQLLHIIKGINVIKLFKDYITGGLRPIFFEGQYCNRLKSLLEKSIYYD
                     IPFYVPSIQENHLQLMNAIVSHLLTSPIPTINISGMCSDWGVSKDKLYSLLYVMDKSE
                     LIKIVQKKGNKHIYSKGGKIFLGDPSMYYCFNGNIGSAREAFIVFSLGEKYPIFASDN
                     EKEYDYQVGRYTIEVGGRNKKNKKADFVIKDDIDIPYKNNFPLWMIGFCF"
     gene            complement(25863..26330)
                     /gene="moaC"
                     /locus_tag="PLV81_04660"
     CDS             complement(25863..26330)
                     /gene="moaC"
                     /locus_tag="PLV81_04660"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOM09423.1"
                     /translation="MDFTHYTDGKPVMVDVGGKEVTSRIARAAGCVYMQKETIEKIQK
                     NLIPKGNPFDVARVAGILAAKKTHELIPMCHPLMITHVDIAIVCDDNNSRVVIEATVR
                     CDGKTGVEMEALTAVSVAALTIYDMCKAVDKQMVIGDIRLVEKHGGKSDYVEK"
     gene            complement(26345..26743)
                     /locus_tag="PLV81_04665"
     CDS             complement(26345..26743)
                     /locus_tag="PLV81_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09424.1"
                     /translation="MKKIVYVVVLLLLCNISAFAKDFLSNQDSRDRFIRLEVIVDEGF
                     KSTNIRIDNLRDDIKDIKTFMLWGFGILFSGMGILIGFVLWDRRTALAPVIKKYEELE
                     ERQEKIEHSLKELAIQDKKVGDTLRKVGLL"
     gene            complement(26941..27654)
                     /gene="pssA"
                     /locus_tag="PLV81_04670"
     CDS             complement(26941..27654)
                     /gene="pssA"
                     /locus_tag="PLV81_04670"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOM09425.1"
                     /translation="MSVAWIPNTLTLGNLTLGFISLIFASMNIPHHLTIAGILILAAA
                     LLDGLDGQVARMLGVASELGKELDSLADCVTFGVAPGFLAYKGYLQGTWVQAFGQSID
                     AGVFIAAIFPVCAAYRLARFNVTHVPNAFDGLPSPIAGIIVALVPLCFLNIHIPKIVF
                     IISFIVVALLMVSTVRYSKPQSYLLEKITGIKLFLFIIVFIALIVIFKQWVILAIIAL
                     YILSGILSFIIQVIQDYRY"
     gene            complement(27744..28424)
                     /locus_tag="PLV81_04675"
     CDS             complement(27744..28424)
                     /locus_tag="PLV81_04675"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOM09426.1"
                     /translation="MSYRHKNTTVLHCEAVCFDMDGVVADSMHAHAQCWIDVFREHGV
                     HLDKTDIFKREGMTGLESIADIFRDKGFAMPPEHILKDLQKKKLQLFSNYQIEIFPQM
                     YSIFPLLKSKGKKIGIVTGSFRYLVFELIPQELMHYVDVIISVDDVTKGKPNPDPYIE
                     AVKKLQVKPEHVIVVENAPMGIKAAKAAGLFCIAISTTLGAGFLKEADVIMQDHNELV
                     AYFTHCLS"
     gene            complement(28447..29604)
                     /locus_tag="PLV81_04680"
     CDS             complement(28447..29604)
                     /locus_tag="PLV81_04680"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HOM09427.1"
                     /translation="MNKKSGGNSPVKKTTGKKLSLGIIYGGKSGEHDVSRCSAASVAL
                     VASKKYRVVCIGITRQGLWYVQDGINVETHPSFGQVLSLKETGTWLINMHSATDTLEL
                     YNKKTGQTVRTEVVFPLVHGTYGEDGTLQGLLDMVGVPYVGPGVLGSAVGMDKDIAKR
                     VIQHSGLVAIVPFETVRTDDMQSSDDMLLQKIAAMLPVFVKPANAGSSVGISKVTTIP
                     DLQKALHKAFAFDSKVIVEKAVDCREFEVAVMGNEKPRASAVGEVIPQHEYYSYEAKY
                     VDKDGAKLAIPANIPDALALKLRQAACSVYTMLECEGMARVDFFVDKHDGTIYFNEVN
                     TLPGFTSISMYPKLWEYSGVSYPKLIDTLISYAFRRHRVKKKMVKAFLSMK"
     gene            complement(29597..30820)
                     /locus_tag="PLV81_04685"
     CDS             complement(29597..30820)
                     /locus_tag="PLV81_04685"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOM09428.1"
                     /translation="MPMKLIVQKYGGTSVGTVERIKAVAARIKRYVENGYAVVVVVSA
                     MGKTTDQLIDLAKQITRNPNKREMDMLLSTGEQVSIALLAMALHEIGINAISYTGSQV
                     KVITDGNFSNARIQSIATDRIQKSLEENKVVIVAGFQGIDQEENITTLGRGGSDTSAV
                     ALATVLGTRDCEIYTDVDGVFTADPRIVPGTKKLKEISYEEMLELARLGAGVLHSRSV
                     EFAKKYNIRLHVRSSFNDNEGTIVMPREEMMEKFVISGVTSKDDEAKITIRDIPDRPG
                     VASLLFGELGKQKVYVNMIVQSTGKDGKASISFTVLKNDLDKALKICESLKEKLGASG
                     IDYKENIAIVSAVGVGMLSSWGVAGRMFDVLSKHNINIEMISTSEIGISCVIDAMYTE
                     LAVKAIHKEFIESNE"
     gene            31178..32050
                     /locus_tag="PLV81_04690"
     CDS             31178..32050
                     /locus_tag="PLV81_04690"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HOM09429.1"
                     /translation="MKIDSSFVGTPLKDYTTTVTWRATMNYAAAVNDANPVYFDDEEK
                     TVIAPPMFAVALTWPISERIWEYIDDKNFPVDIIKTQVHYTEHLAFHRPMYAGDVLTI
                     KGHIAAIMAHRAGTVVVIRFDAIDQDGNGVFTEHIGGLMRGVECTGNSRGKEDLPLVP
                     PFSGVPGAQWEKVIHIDRMQPFIYDGCTNIHFPIHTSVGFARIVGLPDIILQGTATLA
                     FAARELTDNEAAGNPQRLKELSCRFTGMVVPPADIRVLLLQREEHASGTDLFFEVHGK
                     EGRVLSNGYARISG"
     gene            32123..32764
                     /locus_tag="PLV81_04695"
     CDS             32123..32764
                     /locus_tag="PLV81_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOM09430.1"
                     /translation="MKRVAKKNSRGQSRKPPSPLPEKLKQRLYPAVLELFADNDFHHV
                     NIREIYRKSGISPSTIYRYFPSKEDLLFTILDEKISEIGVLVREHIKGIESTKEILRK
                     IFWVTMDYYDRNPGVAVTAFITVPMRTWMKERSYRRDDASAIIEEVVKRARDRGDIDP
                     SIRTSQILDQYYMYCYRQIHLWYFNGMTTRLVDRIPAFFDILWKTLSPPEHGS"
     gene            32912..33919
                     /locus_tag="PLV81_04700"
     CDS             32912..33919
                     /locus_tag="PLV81_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HOM09431.1"
                     /translation="MKQWLYKHSIYCILAMIFVPVAIAQSYPKISAYMPSYALYTESG
                     QNKTTRYIIIRRLKYNSVQAYVYINPDTLHTGILKNPKGTLQQNSLQNIIQQCADTQY
                     AKLYQNALQKSKRMFNAGITHVDTHGMILSFDLCPSKRFLDTDCIEQLIALNKIVPLP
                     VAICISGKWMLTHPEDLQWLQSLASAHQLDITWVNHSFTHFYSRKLPVRKNFMLKEDT
                     SVAREVLQNEICMLEHDIFPSIFFRFPGLISNQKIYEKVMGYGLIPLGADAWLAKGKR
                     PVTGSIILVHPNGNEKKGTYILIQLARSGLINITNIVSLTAACTNSSQTASIHKKQVK
                     L"
     gene            complement(33954..34553)
                     /locus_tag="PLV81_04705"
     CDS             complement(33954..34553)
                     /locus_tag="PLV81_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOM09432.1"
                     /translation="MGRRILKDTRRGEIAQALYRCLLRKPFARTTIKDIAKEAKLNHG
                     MLHYYFKNKEEILLYFLDWIVALHVDDFKRWAQKQKLESRNFAEALSLALDYAKNKIT
                     LNEELAKIFIEVWEIALYHKKVRTKLQYVYKEWIEQLVAIIGKETTKKSATTLSVATI
                     AFLEGISLFHIMLSDIYPIKDILNSFEKKIESVINKRNI"
     gene            34750..36162
                     /locus_tag="PLV81_04710"
     CDS             34750..36162
                     /locus_tag="PLV81_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOM09433.1"
                     /translation="MALWKDTLTDNDKKLIFSLFSRHISKGQTKTMRAAHLDFIETRR
                     KGIGFIDGLFGRKIIDCFTSAGCFNVGRGNPEIIKALNEALNTYDIGTNLLQSNVKKE
                     FIKKLASIAPGNCNQVIFASGGGDAIDCAIKLARGATGRKKIIAMKKAYHGHTGYSLS
                     ANGKDYYRHLFEPLEPNFVFVQFGDLDAIRQVAGIDTAAIILEPVQGEGGIFVGDDYY
                     LKSLRTICNQFSIMLIFDEIQTGFGRTGRMFASQHSGVVPDIMTLAKSISGGLSPNAV
                     VMYRNIPLLTQYVKSNPEFHQSITGGSDIGCAVGIAVIDYIVKNRLWENAESMGNIIM
                     EGLLSIQHENPDIIKEIRGRGLMIGVEYTHEYMGALMADSLARHGIFAVYSGNQPQVM
                     RFMVPITIHEQEVKKMLDIIRKAIATMRMYMPLTKPLSKIPLLKKLLDDIHVQIAVFD
                     IIRYFEDILGITKRNYSKEA"
     gene            36164..37309
                     /locus_tag="PLV81_04715"
     CDS             36164..37309
                     /locus_tag="PLV81_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOM09434.1"
                     /translation="MDLQSLFEGKRNGEFFYDTKGKRYIDCYCGAGTFNVGRHVQELS
                     NTLQEQADKSDQGNFVLISQEKALLASKLISFIPAKLNGVFFSAGRGEAMDFACKLAR
                     GFTGRTQLVTVDGGYYGETGFSVSLSDGDKKKSFGQIPDIVSIPFGNIQALEERITAK
                     TAAVITETIQVENACRSASEGFFKIMRTVCNRTGALMILDETQTGMGRTGKKFAFEHY
                     GVEPDILVIGEALGAGIFPIAATAFTRKIQKFMNQHPLIHLSTFGGHDLGCRVAYEAL
                     ELYEKTKPWENAGNVGQKLLEELAALQKQYPGKILSVHGRGLVIAITVRSDVMAQKAV
                     TKLVKEGIFTATGKVARDAVIIRPALTISEETAKTIARSIRRVFKTL"
     gene            complement(37311..37444)
                     /locus_tag="PLV81_04720"
                     /pseudo
     CDS             complement(37311..37444)
                     /locus_tag="PLV81_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189145.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            38112..39473
                     /gene="rho"
                     /locus_tag="PLV81_04725"
     CDS             38112..39473
                     /gene="rho"
                     /locus_tag="PLV81_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004765954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOM09435.1"
                     /translation="MTKVSNETVEKNNNNNRKNGNGNGDYAKKGNGTIQEGRLDLAEL
                     KSKTISSLMEIARSMGIEGVSGLKKQDLIFEILKAQTERSGLIFSSGVLEVLNDGYGF
                     LRSPNYSYLPGPDDIYVSPSQIRLFGLRTGDTVAGQIRPPKDNERFFALLRIEAVNYE
                     NPEKLQNRVLFDNLTPLYPETRINLETSPDEIDMRILNIIAPIGMGQRALIVAPPRTG
                     KTIILQKIANAITTNHPDIILIVLLIDERPEEVTDMERSVKGEVISSTFDEPASRHVQ
                     VSEMVLEKAKRLVEHKKDVVILLDSITRLARAYNQVVPTSGKILSGGVDSNALHKPKR
                     FFGAARNIEGGGSLTIIATALIETGSRMDEVIFEEFKGTGNCEIVLDRKLADRRIFPA
                     IDINKSGTRKEELLVDAEELNKIWILRKVLSSMSSTEAMEFLIEKMRGTKNNKAFFKA
                     MQG"
     gene            39513..39713
                     /gene="rpmE"
                     /locus_tag="PLV81_04730"
     CDS             39513..39713
                     /gene="rpmE"
                     /locus_tag="PLV81_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOM09436.1"
                     /translation="MKKGIHPEYYDTVIKCACGNEIPTRSTVKDLHVEICSNCHPFYT
                     KKQKIVDTTGRVEKFKKKYNIK"
     gene            40233..41069
                     /locus_tag="PLV81_04735"
     CDS             40233..41069
                     /locus_tag="PLV81_04735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOM09437.1"
                     /translation="MKKYNDFEDLSINQYFNAINQVELLTPEEEIELAKKKCKGDEKA
                     KEALINANLRLVVKIAKNYTNLESSLIDLIQEGNLGLIRAAEKFDYKMGCRFSTYASY
                     WIKHYITRFIAKRSRTIRVPIRKGDLFKKIKRAQDELTNELGKEPSYKDIADMLGIDE
                     RSVSDILEVFQPTVSLEYPLNDEEFNLYEVVSDEKFLSPDTSISRRDLKKELEIAMES
                     LMENERLILTKRFGLDGNEPVTLKDVGNEFGISAETVRQIEARAMQKIKQRFAYLADY
                     IE"
     gene            41282..42511
                     /locus_tag="PLV81_04740"
     CDS             41282..42511
                     /locus_tag="PLV81_04740"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOM09438.1"
                     /translation="MKNYKKLIIFSILLVVCIAIIAYLTDGYQENDNTSMLFFNGNII
                     TVDSTTSKPEAVFIKGSRIYFTGTLQDARKLTDASTITINLKGRTLIPGFNDNHTHTL
                     AAGSFYSEAILWNKTCEEIAVIISQEAKTKKPGELISGNSWDYTTCPKPHKSLLDKAA
                     PNNPVFLTQYSGHAAWVNSRMLEEMGIDKNTPDPKGGQIVRDDKGEPTGILRDTAMGT
                     SEYGKFIKQILSPSLHKKLITKALTLYKEAGITSVQDNTWEPFTVRLLKKYRNSQELS
                     TRFTCWPMGNSPLYYAFTWFASFDKNDYWVRKIFYQPLLGIYYAVTRKSKDGTMGPLN
                     KDECFSPYEALYCYTMGSAYAEFMENEKGSITKGKLADFVVLSDNLLTVPVHTIKDIK
                     VLMTITGGRIVYKHNDF"
BASE COUNT        13328 a         8601 c         7435 g        13272 t
ORIGIN      
        1 accagggtat ctaatcctgt ttgctcccca cgctttcgcg cctcagcgtc agttttggac
       61 cagagagccg ccttcgccac tggtgttcct cccgatatct acgcatatca ccgctacacc
      121 gggaattccg ctctcctctt ccaaactcaa gagaaccagt atcaagtgca gttcatgggt
      181 tgagcccatg gatttcacac ctgacttagt cccccgccta cacgcccttt acgcccaata
      241 aatccgaaca acgctagccc catacgtatt accgcggctg ctggcacgta gttagccggg
      301 gctttaggca ggtaccgtca tcgctatgga tattagccat aacttattct tccctgctta
      361 ccggagttta cgacccgaag gccttcatcc tccacgcggc gtcgctgcgt caggctttcg
      421 cccattgcgc aagattctta actgctgcct cccgtaggag tctgggccgt gtctcagtcc
      481 caatgtggcc gttcaccctc tcaggccggc tacccatcgt agccttggta ggccattacc
      541 ctaccaacta gctaatgggc cgcggactca tcctcaggcg ataggtcccg ataaatcggg
      601 atccccacct ttaatggctg gaacttgcgt tcctaccatc ttattcggta ttagctccag
      661 tttcccggag ttatccccaa cctgagggta gattatccac gtgttactca cccgttcgcc
      721 actctcaccc taatgcaagc atcagggatc ccgtgcgact tgcatgttta agacgcgccg
      781 ccagcgttcg ttctgagcca gaatcaaact ctccattata gaatataata aagagtttac
      841 attctaaaat taaattatta taggtttcaa aacctatttt ctacaaagat gaaaccattc
      901 acccgtgaga tactactcag ttttcaaaga accatgtttt tggataaaaa atcccactca
      961 tgtgggagag ttgcttaaag tgttctgaaa gcttttcttt aaatatactc gctttatttt
     1021 agttcgtcaa gtttttctta caaacttttc gattttttta aagctttctt taagaaattt
     1081 tttatacgat ggttaaaaag aacacgtcag cttctatcgt taagcgatat gtacatatag
     1141 ttaaacacta ttgtactgtc aagctttttt gataaaaaaa ttctttaaaa aaaatttttt
     1201 ttagtacatc ctcacttcag tattaacagg atgtagtctt ttaaatcaac gttttgtgac
     1261 catacaaaat taactatata attgtcaata aaaattataa aaaatatcag tatttattac
     1321 tcacaaaatt caatctatgg caccatattt tatctttatt tcttctaatt ccttctttaa
     1381 catgggcacg atttttttgg ctttttgtgt gccaatcata ttattcatct cttttggatc
     1441 ttccaataaa tcatatagtt caggtcctct acaagcttgc cattctatgt acttataacg
     1501 ttctgtacgc agagtcttgt ttggaggaac cctccctcca aaagggaaat ctttaaaaag
     1561 ttcatacata aaggactttc ttcccggtga aagtgggttt tttacaattg ggacaaaact
     1621 ttcaccctgc atccaatctg gaatacgaat accagctaac tgtaataaac ttggagccag
     1681 atcaatattc aaaatcattt gctccctttt accaggtttt ttgacaattc cggggtagaa
     1741 aaaaataaac gggatcctga ttgattcttc ataaggccag tgttttgcat aaagtcgatg
     1801 ttcgccccac atatatccat tatcgccagc atatactatt accgtatttt ctaagatgcc
     1861 cattttattt agtagctgaa taatacgacc aatttgttca tcaacagaaa ccaggcattc
     1921 gcagtatcgc ttgtatatat catgcattga gcccattgac ccttcaagcc agttatttcc
     1981 actccatgtg ttccacttat ctgattctgg tgcaaggaaa tcaatttttt catttttata
     2041 taatccctta agatgccgtg gcggtttcca atcatgatga actgctttat gcgaaagata
     2101 cagacaaaat ggattttttc ttggttgaga aatgaattca agagcataat ctgttaattc
     2161 ttcagtaata tattctttat tattagagac tggcttatga ttaacaaaaa gtggacaatt
     2221 ataataatca ccctgcccat cttttcttgt aaaggaaata aaaagatcaa caccccgtaa
     2281 atgaggtaaa cctttcccgg gcatatgcca ttttccaatg aaagctgtat catatccacc
     2341 ttgctttaac aattccataa atgtcacatt ttcagctttc catggtgaaa aattatttat
     2401 aacaccatgg cgtgatgcat atttacctgt taaaaaactt gctctacttg ggctgcacaa
     2461 tgaagtggta acaaatgcat ttttaaaaag tattccatta tttgccaggt aatctagatt
     2521 atatattatg gatttttccc accattgtgt caatactatg agctatcgca catactataa
     2581 attttttgaa atatatttgc catactatta caattattaa tgaatggctt taatacaata
     2641 cggtgaagag tccataccat gatcagtttt gacttagaat gtaataatgg gcataaattt
     2701 gaaggatttt ttaaggatta caatgccttt gatgatcaga tgaaggcagg ccttgttgaa
     2761 tgcccggtgt gcaattcgcg cgaaataaag agactgtata ccggatgctc aatacaggca
     2821 aaaagctcat tacttgaaaa aaaacaacca accttctttg aaatggttcg catggttgaa
     2881 tcatatatca aagaacattt tgaaaatgtt ggcgacaggt ttcctgaagt tgcccgtgca
     2941 atatactatg gtattgaaga agagcgcaat atttatggta atgcatccaa agaagaaatg
     3001 aaagagttga ttgacgaggg gattccggta ttgcctttgc ccaaaattga tgatatagaa
     3061 aattaataag ctatcggcgc tgactcaaaa gcgaaaacac gcataaccct atatatacta
     3121 ttgaaatcgc aatattaata taatacccat acgctaattc atatggtttt gccctggtga
     3181 taagattgcc tgcaggcaga tatatcatcc acgctaaata gtatacaatg atgataagcc
     3241 ttgtaatcaa tatccagggt ttttgtacta tggttgaaat caccattatt atgataatac
     3301 cgataaaata aacagccagt gggttattaa cataatacca gctttcaaaa atataaaatc
     3361 gtgcaatagg caaaatgggt atgataactg atactattga aagtaaaata attatttttt
     3421 cctgtatttc tagctcccga aaaattccaa tacaataatc tatcaaccgc atcaaaatat
     3481 cagctaccca attaataaat ttcatactat acgatagtag ctttgcaata aagtctaaaa
     3541 taaaatcaag catacctgga gtcctttttg taatattatc ctaaaagatt gtaatcactg
     3601 actatataaa aatcaattga cttaaatacc aatatgccct taggttgacg tatatacaat
     3661 ggggctgtag ctcagctggg agagcgctag aatcgcactc tagaggccgt cggttcgaat
     3721 ccgatcagct ccaattgtgt tacactactg tttctggatt tctttcactg gttttattaa
     3781 aaaaagctca ttcctcttgc tcaaattaat atccttaaat gaatcttttg ctacaaactc
     3841 attataataa tgggaagctg gattgctgga tatgctcgtt gatagagaag cccggtgaac
     3901 cattttatcc atatagaaac atgatacggt tacagtatca acactgaagt ttttttgctc
     3961 agagagtgac cggtatacag tagaagcaca cccattaaag gctattgttt tctttttccc
     4021 cattacattt ttaaccagtg aacctgaatc aagataatgt attgttgcgc tgtgaacttt
     4081 gccccatttc cattcgtcaa tatacgggcc tattgtatag ttaaaaggtt ttaatgaatg
     4141 caaaaatgct ctatcaaaaa tcatatcccg cgattctatc ttttctagag ttgttatatc
     4201 atcaaaatat actgatctat cctccttaaa aatcctttta aactccggtt caaaaattga
     4261 atagtgtgta agcatattct ttactgcatt gggtaactca tctgcaaatg tttcccgaat
     4321 cattgcagca ataatggcat tgaagagcgt gggagccacc tcactgcttc ccatggtcaa
     4381 atcccaatcc ctaaaataga cttttgccaa cttagcagac gtaatgggca ttttatccag
     4441 caaattattg ataataacca gatattcctt agcatcaggg tatgtaacgc tataaagaat
     4501 tttttcaata tccttatatt gcatctcatt gtaatgtgac agttcatggg atagtgagga
     4561 taacctacct gtattgttaa ataccattac acgctggatt gcgggtgatg ggttttcaac
     4621 aaattcactc ccagccatac acatatcagc ctgttttatt tcaaataagg caggagcatt
     4681 ttttaacaat aatgccttct ccgggcttag caatacagat tcattatcga gcatcgcact
     4741 tccggcaata atctgatatg acctggtatc atcctgcaca ataaatacac gcggctggcc
     4801 tttatatttc tgaacgatct gtattacatc ttgtattgaa tttgcaaaag caacatcaat
     4861 catcgactca atataccctt ttgcagggga cacatacgat attgctacaa ttgcatcttt
     4921 cagattatat tcaaaagcat cagatataac cgggccacta tcggtaaacc gtgatacgca
     4981 ttgcgatgca tcaggctgac attgtaattc tttataccct gcagcactgt tatagtacaa
     5041 tacattattg cgtgtttgtg tctgatattt gcaaaacacc tgactgtcag caaataatga
     5101 gaatgaagca tagcggagtg tattggtttt ccctgcaaca acatacggca atcctgcaca
     5161 tgtataccct tcaataactt tgcctgcaac ctgaaaattt acaggatata tcatggggta
     5221 cagagaaaca ctgtgctcat aacttgaaca cagtgtagca gaatcagtgt acatcaaacg
     5281 tgaggggata gcaacagcaa atcctcttgt atatgaacca tatagcccaa aaattgatgc
     5341 aacaacttct tgcagttttt gtatctcttt gataatagtg aaatcctgct ttctgtagct
     5401 atggacaatt tttttattag ctaattcacg gtaaagcagt ggaaaatctt tatcctcaac
     5461 aggaaagaaa agttcactgt ttgtaacaaa cgcattacag aattctaaaa acagggtaat
     5521 acaaacaata tcttcaacac tccagtgtgt tttattttta ctggcatgcc ccatcttaaa
     5581 tgtataggta ttgatgccat ccacatactt ttgcaaagtc tgtaagtact gtggcgatag
     5641 ttccgacaat aacgtctttg cttttaaggg caatcctaca gctttgacca gcctgtctat
     5701 gctttcgtat tgagacaaat catcctggac acctgtagca gttgcaagac ttcgcaaaaa
     5761 ctccatttgt aagagcctat cctgtgcatg aacaattcct aatgcaaata ttgcgtcatc
     5821 acggttttct gctacaatca gcggatagcc tttatcaagt cgataaattg ctacagggaa
     5881 agcaacacct tcggttatgg tatcctgata cgaaacaggt aaaaaataaa accggtaaat
     5941 gccaattgcc agcagtataa ttgcaacagc accacctgta atcagtaaaa gagtttttct
     6001 ggtcataggt ataaccaccc attaaaattc aaatgccagt ccccctaatc tggtttccac
     6061 ctcttttaaa atacgctgtt catcattctg gtcctttgct tcaaacgtag ccgaaaagcg
     6121 gacaaaatta cccacatcat cccatggcac agaggatatc aggcactctt ttattaaata
     6181 ttgcgaaaaa ctttctgcat tgtcaaaaac aattccactt tttgtttttt ttggtgcctg
     6241 cacatacaga tagaatgttc caccaggcat gtgtgcatca aagcccaaac gtcgtaaggt
     6301 tgtaacaagt gaagatagtc ttcttttata cttttctttt attttttcag ttagctcagg
     6361 atgttccagg gcagttattg cagccttctg aatggccttg aattgccctg aatcataatt
     6421 atcttttaca tttgcaaatg cttttactac aagagggttg cctgcaacaa aagccattcg
     6481 ccagcctgtc atattaaaag cttttgatag cgaatgtatt tcaaccccaa cttcttttgc
     6541 accggggata cttaaaaatg atagcggctt cccattatag gtcaaggccg cataggcggc
     6601 atcctgtacc accataatct tatttttaag tgcaaactca atgacctttt tataaaaatc
     6661 ctcagtagcc actgctcctg taggattatt ggggtaattt ataacaagaa cttttgcctt
     6721 ttttacaata tctgcaggga ttgaatccaa atcaggcaaa aaattattat tttttaataa
     6781 tgggatatta tacacctgcc caccatacca ctcggtatga gtaccaaaaa ctggataccc
     6841 gggaactgtc attaaacata catcacccgg attaataaat gctgaaggca acatggcaag
     6901 tgccggcttt gaaccaattg aatggtttac ttctgtttca ggatcaattc cctcaactcc
     6961 aaatacttct tttaaatatt tagctgctgc atatttaaac tcaggaatac cattatctgc
     7021 atacccacgg ttttcatgtt tataggcttc tttacaaagt gattcaatta ctatatcaaa
     7081 agctttttca tcaggctcgc caacacctaa atcaatgagc tctttccccg gattattctt
     7141 gagagcttca cgcttagcca ttttaatttt ttcaaacttg tatatctttt caactttccc
     7201 aaattggcta ccacctatcc gatctgcaaa taactcctga atatacgact ccatagatat
     7261 ctccttaaaa attataatag tctcatttca atttagaaat attggattct accagttcca
     7321 cagcatattt attgccagga agttttaaaa atctgctgaa acggccaata acgtaaattg
     7381 tatcaccaga ctttaaccgc gcaactgacg taaaatcttc ttcattgcgc cagtttcttg
     7441 tatagatata gcaatctatt ggccctgagg agtgttccac agttgctatc acactgaatg
     7501 tatagtcaat gtttatggaa ttactaattt gtttgacaat agcattgttg accgtaacac
     7561 gttttctttc cagttgtttt aaaagtaaaa agcctttagc agggttttca gctgcatcaa
     7621 tttgggcttt tattgcaatt acttcatcaa aagaatatga cggaatccag aacttcattg
     7681 atgtgcagcc agtagtaagt agtaatgcca gaataatgta tttgttaatt ttcatcataa
     7741 actcctctga aaatagaatg acaatacgga cctacaaaac aattcactat aacaggttgt
     7801 caagaataat acgcaaaaca gttttttgtc aaataagtta ttggtttttc cggaaagtta
     7861 atggatacat gtaagaaggg aataaataag ctatcgttat cattatttct ttttctgtgt
     7921 tttggcggaa ttatcagata cagcttcata actatctaaa atcttgtaca tctcccagaa
     7981 tgagaagaac aaagtcaaaa gaattggccc aataattatg cccggtaatc caaaggtctg
     8041 caagcttccg agtaacagga aaaatagtat gagaggatga aaattcagct tttcaccaaa
     8101 aaccactggt ttcagtacat tctcaagcag aagataccat cctaaacacc acagggctat
     8161 aatcaaagct ttgacggggc tacctatcgc cagtaaatac agagacgctg gtaaccatac
     8221 aaaacttgtg ccaataaccg ggatgagtga aaacactgca gcaatactac cccagagcac
     8281 cggcgttgaa agacccacaa tcacaaatcc aatacctaca aacaaaccct gtaacatcat
     8341 gatcatgaaa ttgccagcta aaagaatccg gaccaccatt ttcagagtat caaccatatc
     8401 gtgctctatg tcattgggaa acggcaatat tgtataaata cttttttcaa accgctcact
     8461 atccttgaaa agaaagaata gtatgagtaa cataaagaaa aagtttatgg aaaaccgtat
     8521 ggaaaatgtg agcaacccgg taaggttaga aaatagtgtc aacgacatat cctgcatgta
     8581 gcggacaata cgctggaaaa gctcatcctg tgttatatta aagtatttca gagtttcttt
     8641 caaaaacttg ctgtgcataa ggtattgctt gaaaaccttt gggtcaaatt gcaactgcaa
     8701 aaaattataa aactccataa cctgatcact gaggctgata aggagaataa acgtaggaat
     8761 gagtatggca acaataatga gccccaccat ggcaaagctt gctagcattc tactttttac
     8821 gtactgtagt atccaggtat agagaggctt taatccaata taaaatataa gggcaaacaa
     8881 aaaaggccag aaataatacc ggtaaatata cgcaatacca gcaataagtc caatgaatat
     8941 aacccagaat attgttgtta atcttttact ctggaccaca atcttgtatg aatcccgtgg
     9001 tatcatgttt caaatccatt ggtaagagtt tgcgatagtt actgaaatta aaatgtatcg
     9061 gcctgccagt agcaggccga taccagctga tacatcagct aacctacaat actacttcgt
     9121 gagataatgt gataaatccg ttgcaattcg gttaatggca tgttccagat atgcctcatc
     9181 ggatatcttc ttccgatatt ccaggatttt tggtgatact tccttagcaa ttttcttctt
     9241 tatctcacga tcctccttgt tcatgagacg tttcatatcg ttaccccttt tcttggtcac
     9301 ctgtccctcc ttgaacaggt tagctgacct catataaaca atcggttgaa ttgcaatttt
     9361 atataacatt tttttagcca tatccacacg gtcacctgga aatatacttc tggtgaaaat
     9421 agcatttaat ataaaataaa ttactacctg atgggataga aaagtaggca tgtatttaat
     9481 ttgacgatat tcacatattt accacaatga cattgaacta tacatcaaag aatggtatac
     9541 tatgaaaaaa tgagcacagg tgttgactta aaagtagtca agggataatt attggaagta
     9601 tgaatactat cataaccaaa tttattattt tccagctctg tatctggctg ccaattactg
     9661 caggtgcata cttttcctca aaacaatata ttgatgtcca gaagctgtcg caacgcctga
     9721 ttaccataaa ccttattagt cttgaatcct ttattgtttt atggaccatc tggggtcttg
     9781 atctgacacc tgaccttata atccttccat ttgcaggatt tacagaggtc attttagggc
     9841 ttttagcagg cattgccata tgcaccctga ctcaatgcaa aggcatacaa aaacacacgt
     9901 ttattataag ttcatccctt gcaaatcatg gtttcacaat gggtgggttt ttatgctacc
     9961 tgtttatagg ggaaaaaggg ctggcattag catttatatt tattatttat tttatgccgt
    10021 atgtaataat ggtcatcttt tcctatgcac ggtatgttgg taccaacgcg cctttctctt
    10081 ttaagcgcta cattcttaca cctcaaaaca tgccattgta tgcagtgttt gctgccttgc
    10141 ttcttcacta ttttgatata aaaaggccta tcattaatat tcctcttgac cctatgcttg
    10201 ctacatctct caccatttat tatttttcaa tgggcatcta ttttaaattc aaggatatca
    10261 agggcttctg gaaagcacat ataatgcttg ctttaataaa atttattatt gtgcctttgt
    10321 ttatgtattt tttgttattg tttccacaac tatcgcatga cattaaaact gtaatacaat
    10381 tgcaatcgtt tatgcctgca gctatttatt ctgttatcac atcgatacta tttaatttag
    10441 acaaggaaat ggcaaccagt atgtttgttg tcaatacgat tgttttttca gggataatga
    10501 tgccattact tatcgcaatt ttttagaata tcgactcata cacatttaat ttatataaaa
    10561 ctcacagaca aaatgtatat tggtgtctgg ctaattatca aacaataata gtatcttcat
    10621 gggcgatagt tcctgatatg aatccagata gtatgaaaat actatatcca cttatatttt
    10681 ttaagctcat tgctatgtat ttgttttcag tttgacagaa gattattctg atcatttgtt
    10741 gtctctataa ctttgataat aacattaatc aaatatttgc aatattacta ttttttatta
    10801 catcaaacct tattaatgag tagtatttat tactatattg ataatgtgtt aaacactaat
    10861 gaataccaga ttacaccata ctattataat cgaatatctg ggtaaaattg ctgcttctat
    10921 ctttattttt tattttttta tccagtatat ccatgctgcg ccaccatcac aaaaaggaat
    10981 ctttgacctt agaaattatc aatgggaaaa tggtcctgtt gcactggatg gtgaatggga
    11041 attctattat ggaaaattct acgaccctaa acatttcaag aacttaaaaa acataaatag
    11101 acaatatatg aaaatccccg catcatggag caagtataac attaataacc aaagtctacc
    11161 tgcttctggt tttgcaacat atcgggccac agttctttta agcaacaaca aaccgcactc
    11221 actttctatt ttaatcccaa aacttgacag ttcatattcg ctatatgtaa acgatatact
    11281 tttagtagaa gttggaaaac ctggaactac tcctgataat agtattcatg catggaaaca
    11341 aactatagct gatattccat tgacagttaa tcaaatcgac attaccattc acatttctaa
    11401 ttattactat gcaaaagcag gaataaccaa ttctattctt ttaggaaata aacagtctat
    11461 cttacaacat tatataaaaa gtataattgt taaattcttc atatttggtg cactgttatt
    11521 catatcactg tatcatttag cattatatat ttttaagaaa aacaataagc aatctcttta
    11581 ctttggtata ttcagcttat taatggcact tttatctatc atatttgggg aatttcatat
    11641 tatgcttata ttccccacct tgccctggaa tatactggtt cgtataacat atttaacaat
    11701 gtcattagga ttaacatttt ttgggctttt cattgaaaat ctttatcctg attatttcaa
    11761 tccaagctta tttaaaagct ttcttactat tggcattttg tatactgttc taataattat
    11821 ttcacctgtt ttttactttg caaagctgct atcgttattt ttaggtataa tgatggtata
    11881 tggattatta attttattca tatttgcccg tgcaatcaag aataaccgaa aatatgcagt
    11941 tttttttctc tcagcttttt taatatttgc tctggctatt gtaaatgata tgctacactt
    12001 tttacatttg attaatacaa catatatggt gccgtatggt tttttaatat ttattgtatt
    12061 ccagtctttt attcttgcaa atcaaaattc caggctttac agcaaactgg aaaaactttt
    12121 tagtgaaaaa ataaaactgg aaaatattgc tgcaactttc cagaatcttg catatattga
    12181 cccactcact gaattgccca acaggagaag atttgaagaa taccttcatg ccgaatggca
    12241 tagagctatc cgcaatcaaa ctcctctttc aatattaatg atagatattg attattttaa
    12301 aaaatataat gataaatttg gtcatatcgt tggtgacctg gtactaaaaa aagtaggtgg
    12361 aacaattaaa agttgcatcc atcgcccagc agatatgtgt gctcgttatg gaggcgaaga
    12421 atttattgtt atacttcctg aaactgaaat tattggtgct tatagaattg ccgagcgaat
    12481 tagaaaaaaa gtattggacc ttaaattgcc agctgcaatc accacatcat caaaatttgt
    12541 atcagtaagt ataggttgta caactgagtt cccaaatctt ggtgaccatt gggactatat
    12601 aattgataaa gctgataaaa aattatatca ggcaaaaaaa cttggaagaa accgtacaga
    12661 aaagtaggaa tgcttacaac attgtattag ccaaaatata caataaacac catcttaagt
    12721 cattcccttt ctacaaacaa atgatgattt acagtctgca tgagtgaata tattgcatct
    12781 tcatattgca ccagttgctg gtccttatct ctattatgga taactgcatt aagcatattt
    12841 ttaagtaaca ataaatctga ccttcgcttt atttttttgg cctgtacaag ctctttcaaa
    12901 tagtcaaact tataattacg tagctggaca cgtaaaaaaa attctatgcc atatatctga
    12961 aaaatccttt ctaatgggta acttttactt ttatcaataa attccttata ctgaggtgca
    13021 tctacgactc tcttttttct tctgtcttta actgatgggg aaccttgacc atcatctttc
    13081 cctttctggg attgtttcct ttctaactgc aaatcaatat tggatatact tatctcgtaa
    13141 gctagacgct tagctttcca ttcttcagta aattcatcat aatgcttctc catatacctt
    13201 tcaagctctt caagcatgat agctaaattg gcaaccttcc caatatcagt attgttgagg
    13261 cttcgtaaca ttgggaaaat ttctttaact atctgatgta ccatcaagcg tttatggaat
    13321 tcgttttcat actgtataat aagttcactt tgataaaacg attttaattt tcgtatcaca
    13381 accaggtctg caaccacata cccttccacc ctggaaatat cacggacctt atcctctgct
    13441 ataatctcaa tagaaaatat tttatgggca ttaataagta tctgaatgat acgcttaata
    13501 agatcaatag aaagtccata ataaccagat acatacttta caaattcatc cgaataggga
    13561 acacctttaa atccacctgc agtattacgt tttcggattt cattataaat atcatcaaaa
    13621 attgttaagc tcattttaca accttttatt catcaaaata aaaactcaaa tatgatagcc
    13681 atcatagatg ataaaaaaat aaaagcgttt tttatatttt ttttgtcata ataaaaaaaa
    13741 tgtgattttt tttattgaca gatgttgacg tgattaacct attgtagtat agttttaatc
    13801 aaaaggggtg ttagctcagc ttggttagag tgcctgcctg tcacgcagga ggtcgcgagt
    13861 tcaagtctcg tacaccccgc ataaaaaaag ccgcttatat gcggcttttt ttatccacca
    13921 atttttgaca ttgaatgcat tgccatacct gaacaattaa acgcagtatc atcacattgc
    13981 attaatgaat gtccttcctt tttatgtaat acggcattgg ttataacttc acgtatatta
    14041 tcatttaatt gtcctctgag tatctttttt aaattatatt cagttgcatc atgcagacaa
    14101 gccctgagca taccatctga agttacccgt aaacgattac atttaccaca gaacctgtgt
    14161 gacactgcag gtataattcc aacattaata actgcacgtt tatcccacct gggcaaaaat
    14221 ctgtacattg cagcaacatg tgtatcgctt gttgtcttac gctgcactgt tcctaatgat
    14281 tccaattttt gaagtaatgt atctgcactc acaaatggta ccggatcatt acttaacggc
    14341 atgcgttcaa taaagcgaat tgcatggata tttgttgcaa aacgcaccag ttcttccagt
    14401 tcatcaagtg tagattccat tattacagcg ttaattttac attctacacc ctctaacgct
    14461 gttattttca gtattccgtt tataactttg tgtatggcat taaccccggt aatttttgca
    14521 aatgtatcag gattaagaga atcaagactt acatttactt tctgaacgtt gaattcctgt
    14581 attttatcaa tatattgttc aagtaatata ccattagtag ttatacatat ttctaattct
    14641 ttgtggttgc ttcgtacctg ttttacaata tcaaaaaagc cttttcgtag taacggttcg
    14701 ccgccagtaa aacggacttt tttaattccc agagaaacaa acacatccac cagcctcact
    14761 atctcttcat tacgcaaaat ctcttcatgc ggtaaccatt tgatatctgc aggcatacaa
    14821 tatacacatc gcaggttgca atggtccgtt acagaaatac gcacataatc tatagttctg
    14881 ccaaagctat caacaagcat ttaccacctc atggtacagg aagcccacat ggttccattt
    14941 tcatccgttg tgaatgaaaa ctgattaaag cctacatatg ttggttgcct gctttcattg
    15001 atctcctgaa catggtacca ggtacaaagc caggatgtaa tatatatagc agcatttgcc
    15061 agtatactca aggtaaaata atgagctgct ctgtcatatg catacccaaa ttcttttact
    15121 gttttatagc caccttccgg gtccttgaaa agtaaatcat gttccggatc tattgcttca
    15181 tttgcttttt ttgccgatcg atacaacgaa ccacgatatt gccagtaatt atagtagtaa
    15241 tacatagcat agacacctga aatttttaaa ccggcaaaaa cagcggcata ttcataatgc
    15301 cccgtatata tatgcccacc gcccggaaga acgtcccagg caagtgcctt ccataaactc
    15361 accggttcat cagttttgac aaacgtaaag ggttgttgcg caaacgacgt gtgtgcacac
    15421 aaaattacaa aaataattaa taatacagtg cgatagttca tactataaat ccattattat
    15481 tacaatcaac ctattgaaac catgcctggc ttctttaccg gaaaccacgc atggcgactc
    15541 tacacataca ttacgatttt acggaatatc accatttacc acttcactgt ggtcgctttc
    15601 cctgccacca tcgtgggatg tcatccacac atacaccggc ccatgatagg aagaagaaat
    15661 ttgtatggta acaacctttg ggtcaacgcc ttcttcaaaa tcagtttcag caacgtagcc
    15721 cacgtaatat ttgatatcat aatacaactt tgggtcgctg aattgtgctg gatcagttgc
    15781 tgatatatac accagataat acagattttc attgctgcca gtatcaggat cataggtgag
    15841 taattgtcca ttatctatct tatcagtata aaatgaaagc gtaattatat agttattaat
    15901 tgtcattgat aaatcaactg gcggtgttgg cttctgacca cctttgactc tggaaaggtc
    15961 atcaaagtat tcttcatcaa cgggttcatg caggcaaccg gtagcaagaa ctatcgccaa
    16021 taacaatact attgttttat tgtttaagct catgcaggta caggtaattt tttctaaaat
    16081 atggattatg cggcttcagt actgccgctt tataaatata ctcatagccc ttatcaaagt
    16141 cacccttaag tattaatgca attccataat tattgagcac tgctggctca ccggtaaaaa
    16201 tcgcaggttg taacagggtc agagcacctt ccacatcgcc tttggctaat cggctgatag
    16261 catcattgtt ggtaagcgca atgtcactgc tattgtgtat gtatgctaca taagcagtaa
    16321 aatttacctg tacagatatt gcctggctat cattatgtgg tactgttaca gtccttatac
    16381 atcgtattgt ttcatactgg taaccacacg cagttaaaca aaaagctacg ctaccgtgta
    16441 acacaatcct caatagcctg tgccacattg agcatcaccc tttcattgaa atgttgcgcc
    16501 ataatctgta cccctatggg aagaccctgt gcatcctttg ccacgggcac cgacattccc
    16561 gggatgccag ctaagttagc ctgaatggtg agtatgtcgg ccatatacag tgacagcgga
    16621 tcagaaatct tttcgccaat accaaacgct gttgttgttg ttaccggcgc aacaatgcag
    16681 tcgcactggc tgaatgcttt ggtaaaatca tctatgataa ggcgtctgcc cttcaatgcc
    16741 ttgagatagt aagcgtcata gtaaccagaa ctcaaggcaa acgttcctaa aatgatacgc
    16801 cgctttacct ctttgccaaa accttcctgg cgcgtcttca catacaggtc tttgagcttt
    16861 tgaatatttt ttgagcgata gccgtagcgc acaccgtcat accgtgctaa atttgatgaa
    16921 gcctccgctg ttgctatgag atagtaaatg ggaaccgcat agtcagtgta ttgcagagaa
    16981 atctcctgga taattgctcc ctgcaattct aattttttta acacatcctt gactgcggta
    17041 actatcgcct catcaacgcc gttaaaatat tcctggggga caccgatgcg taatcccctg
    17101 acattgctgg ttatagtggt actatcaaaa tcaaccggct tattgatgga tgttgcatca
    17161 cgttcatcaa tccctgaaat gactgcaagc acatctgctg catctttaac agtgtgagca
    17221 aatgtaccaa tctggtcaag cgatgaagca aatgctacca gaccgtagcg tgacacgcgg
    17281 ccataggtgg gttttattcc cactactcca caaaatgacg caggctggcg tatggaaccg
    17341 cccgtgtcag aaccaagtgc taccggcaca ctatctgctg ctaccgctgc tgcagaacca
    17401 ccactgcttc cgccagggac ctttgttgta tcaactggat ttctggttgg tccaaaaaac
    17461 gatgtttcag tagtggaccc catagcaaat tcatctaaat tggtttttcc taataggaca
    17521 aacccggcat caagcaactt ttgatatgca gtggcattat agggtggtac aaaattttgc
    17581 aaaattcctg aagcgcaggt agttaacacc ccttttgtgc agatattatc tttaatggca
    17641 attggtatac catcatactt tgattttggc tttccctgtt tacggcgcat gtctgattct
    17701 tgagcctgct gtaaggctac atcatgagtg acagtgatat atgcattcaa atctttgtgt
    17761 tcatcaattg ctttgaggta ctccgttact atttcaacag atgtggtttc tccccgttca
    17821 agcatctgga ctaactgact gacggttttt actgccatag agtgactcct gttaaaaaat
    17881 gggattagca aatacattga ttgcaaactc ttaataatgt caaattaatt tattgtatat
    17941 atgttgtaac cattgccaga ttcaaaccac ttacaaaaag tactgataca cacgtatata
    18001 cttcaatagc agaacaggga attatcgcaa acattctata ttctggtaga tttattgtta
    18061 tccaggaata aagtatggcc attgataatt atttgactaa aattacccca cgatatatat
    18121 taggttacat aattatttta gaacgttaaa cgttgaactt tatactaaga acgggtttca
    18181 ttatgaaaaa acatattgta ctgtatttat tagtttctat actgtttttt ggctgctata
    18241 aaaaatcagt tgtccttaat cccaacgata ttcattatat tatcagcacc tttttaaatg
    18301 aacacgtact gtaccatgaa tttgatgata ctatctctca gcgcacattg aataacctgc
    18361 tttcatctat tgatcctggt aaatattatt tttacaaaag tgatgttgat ggatttacta
    18421 aacggtatga aaaccttatt gatgacttag ccaaaaaaga agatttttcg tttcttgatg
    18481 atatttttgg aacatacaaa aaacgtgtcg atgaagtgaa tcatattatt gatggattac
    18541 tcaagcagca ttacaccttt accgaagatg aaaccataaa cttagatagc gataaaatcc
    18601 cctatgctaa agacaaaaat gaactcagag agcgctggcg caaaaatatc aagctacagc
    18661 ttttaaacta tcaaacttca gagagcttaa acgaagacca ggcaaaagaa aaattaaaac
    18721 gcaagtttta ccttaacaat aagcgtattc aggaatttga taaggcaaag cagttttcag
    18781 tttttcttaa cgcattttca atggcacttg atcctcacac caactatctg acgcaggaag
    18841 aacatgaaga cttcatgatt tctaataatc tgaagcttga aggcattggc gttcagttac
    18901 gctcagaaga tggctatacc attgttgacc gcattatccc gggtggagcg gccgacaaac
    18961 ttccaaaaga gttacaattg caacccaatg accgcattgt tgctgttgct cagggcaatg
    19021 atgaacctgt ggacgttgtt gacatggaac tgcgcgatgt ggtgaaaatg atacgtggca
    19081 aaaaaggcac actggtcaag cttaccatca tacggattaa tcctgacagt aaagaacaaa
    19141 agcgaatgat tgtgcccatt gtccgcgagg aaattaactt agaagatagc gctgtaaaat
    19201 ctgaagtgtt tcaacctgag aatgggcctg ccattggtta cattaaaata ccttcattct
    19261 atgttgatat gtcacagcag ggatttggtg aaggcagaag ctcaaccgct gatacactca
    19321 tgcagattaa cagtctgatg aagaaaaatg ttgcatgtat agtggttgac ctgcgtggta
    19381 atcctggcgg agcattgagt gaggcattaa aactggccgg catttttatt gatgcaggcc
    19441 cggttatgca ggtgtacgat agctccggta atattcaacc attagatgac ccaatcccgg
    19501 gagtatacta cagcgggcca cttgttgtac tcattgataa attcagtgca agtgcatcag
    19561 agatatttgc aggagctatc cgtgattatc ggcgtggtct cattattggc accgatgcaa
    19621 cctttggcaa aggaaccgtg caatcatata aagaactgta tcagaaaaaa ggtgctatca
    19681 agattaccaa tgccattttt tatcagcctt ccggtacatc aaatcagctt aatgggataa
    19741 aacctgatat cactgtacct gatatcacaa caatatggga cattggcgaa aacaaactca
    19801 aatatgcatt acagtggaaa cagattcctc ctgctgccta tacgccatac aggaattatc
    19861 ttactgctga tgttattgca cagctaaagg ttagatctga caatcgattg cagaacgatg
    19921 cacagtatgt aaaactttta caggagatca aagatttaaa gcaaaaactg cagtcaaagg
    19981 aaatcaatct aaaagatgaa agtagcattg aacagcgcaa aaaggatctg gaaagctcaa
    20041 ttaaaaaagg gaagaaagat aagcttattg accttgaaaa tgatgtattt ttgcaggaag
    20101 catttcgtat taccgctgat tatttgaaag cattgcattg attgttacag gtcaactgtt
    20161 atatcaaaat ggtgtctggc tttcaatgct gtactataat gtgaaagctg ccttgtaata
    20221 gcatctgcct gcagtattac agcatccgta ccctgcagta aacactgttc atacaagctt
    20281 tgcaggtgct cattacgtga gttcagaaaa gactgtataa aaataacaag ctttatatca
    20341 tagtttggca atgccttgta aataagctca cagcgttcat gcaccgtacg catgccaggg
    20401 caccatgatt taaatgagta agcatacaat agctcatagt acaggttttg tgatgaagta
    20461 tggcatgaaa tgtcaaactg ccgcctccca ttgcagctat catcctcgac aatgacaata
    20521 tcaaattcat tacatgcatc atatatatcc caattgctaa agtcactgta gacaagcgcc
    20581 ttttgagtcg gttcattgtc actatgagtt gatgactcaa ctgcgtggta taactgctgc
    20641 aacaactgca agctctgctg cggcacaagg gaaaatgctg catcgcacag taattgcaac
    20701 tgactctggg tgaatacagc cgcatacatg gtggtcagtg cccgcattat tttgcgtatt
    20761 tcttcataca ctgcaactgc ctgcttgaga ctatcatcat cagggtattc ctgctctact
    20821 cctaaaaact gcaacactaa atacagtatt gtgtgaaggt taaccactgc atcttgggca
    20881 aagccttcat aatgctggat ggcatgtacg gtcaattgct gtaacacttc acggggtatg
    20941 atagtacaat actgtggcac aatgagcaca tcggcaaggc tgcaatactc cactgctctt
    21001 ttatacattg agctgtcggg tttacataat gcagtcatca cccagcgtgg caatttgctc
    21061 acaataaatc catgggctac aaaaacttca tacggcacac tatcgcactg ggtaaaaata
    21121 atctttttgc caccttgctt tatttcacta aagcgggcaa aaaggttatc aagctcttgc
    21181 tgtattgttt caatcacggt tgacttgtcc atggttacta tcgcctcatt aaaaccacat
    21241 tatttttata ataattttct tgtcaaaaga ataattttat tgcgatagtt cattatagtg
    21301 atattcaggc taaaaaacaa accttacatt ctggagaagc tggtatgaaa gcgcaccgtg
    21361 caaataaacc atatctttct attatattac ctgtttataa tgaagaagaa aacattgttc
    21421 cacaatatca gcacattgtt gaagctatgc aaaaaacatc atacacctat gagataattt
    21481 ttgttgacga tggcagcact gataattcct tcaaacttct tgaatccatt gcaaaaaaag
    21541 ataaacatgt gcatgtggta cagttcaggc gcaattttgg tcagacagca gccatggcag
    21601 ccggcattga ttattcaaaa ggtgatatta tcatttttat ggattctgat ttacaaaatg
    21661 atgctaatga tataccacta ttggtgcaga aaattgaaga aggttatgat gtggttagcg
    21721 gctggcggaa aaaccgtcag gataagctcc tgtcacggaa aatcccatca cgcattgcca
    21781 actggcttat tgcaaaagta accggtgtat atctgcatga tctgggatgt tcactcaaag
    21841 catatcgcgg tgagataatt cggcaggtca atctgtatgg tgagatgcac cgctttatac
    21901 cggttcatgc atcatggatt ggagcatcta ttgttgaggt gcctgtacat caccatccac
    21961 gcaaatttgg aaaatcaaaa tatggtataa agcgtacgtt caaggtctta atagatcttg
    22021 ccactgttaa attcatgggt tcatattcca ccaaacctaa ttatatattt ggtggcattg
    22081 gtatgatact gctttttatc agtatcttaa gcgggataac cgtcattgca atgaaattgt
    22141 ttctacatca ttctatgata cgcaatccct tactgctttt aacggttatg cttattatac
    22201 tgggaataat gtttatatta cttggtatat tagcagaaat tatgattaga atttatcatg
    22261 aatcacagaa attgccacca tatcgtgtaa aacgatacat ataaaaagta aaggcaggtt
    22321 taaaacctgc ctttactgta actatcttag tacattcctc ctggaggcat tccaccaggc
    22381 attcctgctt tttcttcttc cttcttttcg gtgataagca cttcagttgt tgccagcatc
    22441 ccagctactg atgctgcatt ctgtaatgct gaacggacaa cctttgctgg gtcaataata
    22501 ccggctttca taaggtcttc ccattccatt gttgcagcat tgaatccaac accctgtttt
    22561 tcctgttttg ccttttcaac aataacagat ccttcaacac cagcattgaa tgcaatcatc
    22621 ttcattggct cttccatagc ttttgcaaca attcgtgcgc ctatcttttc atcgccttcc
    22681 agtttctttt ctaattcagc aacagctttc tgtgcataga gcaatgtcaa accaccacca
    22741 ggcactatgc cttcttcaac agctgcacgg gttgctgaca atgcatcctc aacgcgggct
    22801 ttcttttctt tcaactcaac ttcggttgca gcaccaacgt tgattactgc aacaccgcca
    22861 gccagtttag ctaagcgttc ctgcaatttt tcacggtcat aatctgatgt ggtctcttca
    22921 atttgcttct tgataacttt tattctggcc tgtacatcag cctggctgcc agcaccttca
    22981 attattgtag tattttcttt gtcaacaata accttttttg cacgaccaag catatcaagt
    23041 gttgcatttt caagcttgag tcccagatct tcagaaatta cctgaccctt ggtcaagatt
    23101 gcaatatctt caagcattgc tttcctgcga tcgccaaaac ctggggcttt tactgcaaca
    23161 cactggataa ccttacgtaa tgtgttaaca acaatggtag ccagtgcttc gccttcaacg
    23221 tcttcagcaa taatgagtaa tggtcttcct gcctgagcaa ctttctcaag gattggaagc
    23281 aggtctttca tggttgagat tttcttttcg ttgatgagga taaatgcatc ctcaagtact
    23341 gcttccatat tttctggatc ggttaccatg tagggagaaa tgtaaccgcg gtcaaactgc
    23401 ataccttcaa caacttcaag gtgagtttca atggacttgg cttcttcaac agtgattaca
    23461 ccgtctttgc caaccttatc cattgcttca gcaatcaact caccaatttc catatcattg
    23521 ttagcagaaa tagctgctac ctgagctatt tcttttttat cttttatttc acgtgctgta
    23581 tcaccaataa attttactgc tgcttcaact gctttgtcaa tgccacgttt cagactcatt
    23641 gggtttgcac cagcagttac attctttaat gattcatgat agatagcagc agctaaaatg
    23701 gttgcagtgg ttgttccatc accggcaaca tcattggtct tggtggcaac ttccttaaca
    23761 agctgagcac ccatattttc aaatgggtct tcaagttcaa tttcttttgc tactgtaaca
    23821 ccatctttgg taatagttgg cgagccaaac ttcttgtcta taacaacatt tctaccacgg
    23881 ggaccaagtg ttaccttcac tgcatctgat aacttaatca caccctgata gattgatcgc
    23941 cgtgcttcat cattatactg taaaatcttt gccatgttct tcaccttcct tttctacaat
    24001 gtaatttttt actcaaaaat agccagaata tcattttcgc gaacaatcaa atactccttg
    24061 ccaccatact taacttctgt tccggcatat ttgccaaaca gtacagtatc accaacctta
    24121 acatctaatg gaatccattt gccatcctca tagcggccct tgccaacagc aagaaccttc
    24181 ccctgctgtg gtttttcttt tgccgtatcg gggatgtaaa ggcttccctg ctttggaagt
    24241 tcttcttcca tcacttcaat cagaactcga tctcctaatg gttttattcc cacggtactc
    24301 ctccttaacc ttgattttaa ttttaatatg ataattgtaa tctaaaaata ctgaattatg
    24361 attctttttt aagcctattg tactgagaaa ataagaagtg ttgatattaa ttgttagcac
    24421 tctttttagt gagtgctaac aattaataat atagcaaaaa aaaattttat aacaatttta
    24481 attatgagga attattatta attattgctt tttatagact tttatccaaa aatatgatat
    24541 cgaaatatca gaaataaaaa atattggtct aaataaaaaa atttttaaaa acaaaaacca
    24601 atcatccata atggaaaatt atttttataa gggatatcaa tatcatcttt tataacaaaa
    24661 tcggcctttt tgtttttctt atttctccct cctacctcaa tggtatacct acccacctgg
    24721 taatcatatt ccttttcatt atccgatgca aatataggat acttttcacc taaagaaaat
    24781 actataaatg cctcccgtgc actaccaata tttccattaa aacaataata cattgaagga
    24841 tcacccagaa atattttgcc acctttggaa taaatatgct tattaccttt tttttgtact
    24901 attttaatta attctgattt atccattaca tataataaag aatataattt atctttacta
    24961 acgccccaat cactacacat tccagaaata ttaatagtag ggatgggaga agttaataaa
    25021 tggctaacaa tagcattcat aagttgaaga tgattttcct gaattgaagg aacataaaat
    25081 ggaatatcat aatatattga tttttctagt agtgatttca atctattaca atactgaccc
    25141 tcaaaaaaaa ttggcctcaa accacctgta atataatctt taaataattt gataacatta
    25201 atccctttaa tgatatgcaa caattgttca taatttgaaa aaacatctac aggatcataa
    25261 tatatgcctt cagttaaata tacatattct ctaaaagaca acattggcat tcggtattgt
    25321 acaaacctcc ttgataaatc acccatccct ttttgcaata taagattact gctatcacta
    25381 atccatatat atttatccgg gtaatcatca tatacatttt taatatgcat actccagtta
    25441 ttgctgaaat gaatttcatc aaccacaacc ccatcgtaac cacgagcaaa tatttcttta
    25501 ataatatcat aaagaggata cgcatgtatt gcaagactat cagcagataa gtataaaaaa
    25561 ttcttatctt taattgtatc gataagaaaa gttgttttgc caacaccgcg aggaccatac
    25621 agcaggaaag atcgtatatt ttcagtatct aatgtcctga aaaatggccg ttttctttct
    25681 ggtataaaaa gcttgattcg ctgatatgtc agttctaatt gatcaattac ttctctcata
    25741 gttctattat agaactatat atacataaaa tcaactatta attttaaatt ttttcaaata
    25801 ttttataaaa ttcgttctat attagaacta tttttgatta aatagttctg aaatagaact
    25861 atttatttct ctacataatc acttttaccg ccatgctttt caacaagccg tatatcgcca
    25921 ataaccatct gtttatccac tgctttgcac atgtcataga tagtcaaagc tgcaacactg
    25981 actgccgtta aggcttccat ctccacaccg gttttgccat cgcatcgtac tgttgcttca
    26041 atgacaaccc gcgaattatt atcatcacaa actatggcaa tatccacatg ggttatcata
    26101 agcggatggc acatagggat aagttcatgg gtcttttttg cagcaagtat tccagctact
    26161 ctggccacat caaaaggatt gcctttgggg ataaggtttt tctgtatttt ttcaatggtt
    26221 tctttctgca tgtaaacaca accggctgcg cgggcgatac gagaagttac ctccttgccc
    26281 ccaacatcaa ccatgaccgg tttaccatct gtataatgtg taaaatccat tgtttctata
    26341 ttttttacaa taatccaacc ttcctcaatg tatcgcctac ttttttatcc tgaatagcca
    26401 attcctttaa agaatgttca attttctcct gcctttcttc caattcttcg tattttttta
    26461 taactggagc cagtgcagtt cgtctgtccc atagaacaaa tcctataaga atacccatcc
    26521 cgctgaaaag aattccgaat ccccataaca taaatgtttt tatgtctttt atatcatccc
    26581 gtaaattatc tattcttata tttgttgatt taaaaccttc atcaacaatt acttctaaac
    26641 gtataaacct atctctacta tcctgattgg ataaaaaatc ttttgcaaaa gcagaaatat
    26701 tacataataa taataaaaca actacatata ctattttttt cattattaac aatcacccct
    26761 catgtaatcg ccatcaaaaa tctttataaa ataaagaacc tttgctgtaa acaaaacaaa
    26821 tatacaaaaa aaacatcaat tcagcaaata ttccgctaac ctcagtggat aaacaactac
    26881 tgaagcttta agcattttta aaaataattt ttaatatcca tataacataa atgaaatcaa
    26941 ctaatacctg taatcctgta ttacctgtat aataaaactc aatatccctg acagtatata
    27001 cagggcaata attgccagta taacccactg cttaaatatt actataaggg caataaaaac
    27061 aataatgaac aggaataact taatgcctgt aattttttca agaaggtatg actgcggttt
    27121 ggaatacctc actgttgaaa ccataagaag cgcaacaact atgaagctaa taataaatac
    27181 tattttggga atatgtatat ttaaaaagca taatggaaca agtgcaacta ttatacccgc
    27241 tattggtgaa ggtaagccat caaatgcatt ggggacatgt gtaacattaa accgtgctaa
    27301 acggtatgca gcacatacag ggaatattgc tgcaataaaa acaccggcat caattgactg
    27361 cccaaaagcc tgaacccagg taccctgcaa ataaccctta tatgctaaaa agcccggcgc
    27421 tacaccaaag gttacgcaat ctgccagtga atccagttcc ttacccagtt cacttgcaac
    27481 tcccagcata cgcgcaacct gcccatccag gccatcaaga agcgctgcag ctaaaattaa
    27541 aattccagct attgtcagat gatgaggaat attcatgctt gcaaaaatta atgagataaa
    27601 ccccaatgta agattaccta atgttaatgt atttggaatc catgcaacac tcatatcaca
    27661 cctatgcact atagtatttt tcaaaaccaa acaatgttgc tattatatga ttatttgcca
    27721 gcctgtcaat gaaaaaacag ctttcatgat aggcaatgcg tgaagtatgc aactaattca
    27781 ttgtgatcct gcattatgac atcagcttct ttaagaaacc cagcccccag tgtggtactg
    27841 atggcaatac agaacaagcc cgcagctttt gcagctttta tgcccatagg tgcattttcc
    27901 accacaatga catgttcagg ctttacctgt aactttttaa ctgcttctat atacgggtca
    27961 gggtttggtt ttccttttgt aacatcatca acgctgatga ttacatcaac atagtgcatc
    28021 agttcttgag gaataagctc aaaaacaaga taacggaaag accctgtaac aatcccaatc
    28081 tttttccctt ttgactttaa caaaggaaaa atagaataca tttggggaaa aatttcaatc
    28141 tgatagtttg aaaacaattg taattttttc ttctgtaaat ctttcagtat atgttcaggc
    28201 ggcatggcaa accctttatc acggaaaata tcagctattg actctaatcc cgtcatgcct
    28261 tcacgcttaa aaatatcagt tttatccaga tgaactccat gttctctgaa tacatcaatc
    28321 cagcactgtg catgtgcatg catggaatca gcaacaacac catccatatc aaagcacaca
    28381 gcttcacagt gtagtactgt agtatttttg tggcgatagc tcataatatc aattcacagg
    28441 ttgttattat ttcattgaaa ggaaggcctt caccatcttc ttctttacac ggtggcgcct
    28501 gaatgcatac gaaatgagtg tatctataag tttggggtaa gatactccag aatattccca
    28561 caattttgga tacatgctta tactggtaaa cccgggcaat gtattgactt cattgaagta
    28621 gattgtccca tcatgtttat ccacaaaaaa atctacccgc gccattcctt cgcattcaag
    28681 cattgtatac acagaacatg ctgcctgacg taacttcagt gccagtgcat caggaatatt
    28741 tgctggtatg gcaagcttag caccatcttt gtcaacatat ttggcttcat acgaatagta
    28801 ctcatgctgc gggataacct cacccactgc tgaagcacgt ggtttttcat tgcccatgac
    28861 ggcaacctca aactcacggc agtcaacagc tttttctact attacttttg agtcaaacgc
    28921 aaaggctttg tgtagggctt tttgcaggtc cggtattgtt gttacctttg atataccaac
    28981 agatgaacca gcattggcag gtttcacaaa cacaggtagc atggcagcaa tcttttgtag
    29041 cagcatatca tcagatgatt gcatatcatc agtgcgaact gtttcaaaag gaactatcgc
    29101 caccaaacct gaatgttgta ttacccgttt ggcaatatcc ttatccatac ccacagctga
    29161 acccagaaca cctgggccaa cgtatggcac accaaccata tcaagcaaac cctgcaatgt
    29221 gccatcctcg ccatatgtgc catgtaccag tgggaacaca acttcagtgc gtacagtttg
    29281 tcctgttttt ttattataga gttctaacgt atcagtggca ctgtgcatat tgataagcca
    29341 cgtgccggtt tctttaagcg atagtacctg gccaaacgat ggatgtgtct ctacattaat
    29401 accatcctgt acataccaca gaccctgccg cgttatacca atgcacacca ccctgtattt
    29461 ttttgatgca acaagcgcaa ccgaagctgc tgaacagcgc gaaacgtcat gttcaccact
    29521 tttgccaccg tagataatac caagactaag tttcttgcct gtggtttttt ttacggggct
    29581 attgccacca ctttttttat tcattgcttt caataaattc cttgtgaatg gcctttactg
    29641 ctaattcggt gtacattgca tcaataacac atgaaatgcc tatctcagat gttgaaatca
    29701 tctcaatgtt tatattatgc tttgacagta catcaaacat acggcctgca acaccccacg
    29761 acgacagcat gccaacgcca accgcagaaa ctatggcaat attttcttta tagtcaatgc
    29821 ctgaggcacc cagcttttct ttcagtgatt cacaaatctt taatgctttg tccagatcat
    29881 tttttaacac agtaaaggat atggatgctt tgccatcctt acctgttgac tgaacaatca
    29941 tattaacgta gactttctgc tttcctaatt caccaaacaa cagtgaagca acacccggcc
    30001 tgtcgggtat atcacgtatg gttatctttg cttcatcatc ttttgacgtt acgccgctaa
    30061 ttacaaattt ttccatcatc tcctcccgtg gcattactat tgttccttca ttatcgttaa
    30121 aacttgatct gacatgtaat ctgatattat acttcttggc aaactcaacc gaccgtgagt
    30181 gcaacacacc cgcacctaac cgtgcaagtt ctaacatttc ttcatagctg atttctttaa
    30241 gctttttggt accaggtaca atacgcgggt cagcagtaaa aacaccatca acatcggtat
    30301 agatttcaca atcacgtgtt cctaacaccg ttgcaagggc aactgccgaa gtatccgaac
    30361 cacctcttcc cagcgtggta atattttctt cctggtcaat accctgaaat ccagccacaa
    30421 ttaccacttt gttttcctct aaggatttct gtatcctgtc ggttgcaatg ctttgaatac
    30481 gggcatttga aaaattgcca tcagtaatta cttttacctg cgaaccggtg tatgatatgg
    30541 cattaatacc aatttcatgc agtgccatag caagcagagc aatggaaacc tgttcaccgg
    30601 tggaaagcaa catatccatt tcacgcttgt tgggatttct ggtaatctgc tttgccagat
    30661 caataagctg gtcagtggtt ttccccattg ccgaaacaac cacaacaaca gcataaccat
    30721 tttcaacata ccgtttaata cgtgcagcta cggctttaat acgttcaaca gtgccaaccg
    30781 atgtaccgcc atatttctga acaatgagtt tcatgggcat ttaacctttc tcgtaaggat
    30841 ttagatactc tgtgcgctat ataaagagtg gctgtacaat tgcaactaaa attataccag
    30901 ttattttaaa ataatgtaaa atttttatgg gcgatagtta tgggaaaaag attttatcgt
    30961 aacaatgtta ctaaaattta atcatatcgt aacaatgttt ttttattgac agtacactat
    31021 ttgtatcata tagtaaacgc atgcattgct ttctttacga caggcattta ccatttccga
    31081 cttgcataca gtactaaaaa tatcgaggga tttttcggca aaggaggaaa aaagccaatc
    31141 ttgttgcaat gcacattata ataacataag aaagaatatg aaaattgatt catcgtttgt
    31201 tgggacgccc cttaaggatt acactactac ggtaacctgg cgcgcaacca tgaattatgc
    31261 tgcagcagtg aatgatgcca atcctgtcta ttttgatgat gaagaaaaaa ctgttattgc
    31321 gccaccaatg tttgcagtag cgcttacatg gccaatctcc gagcgtatct gggagtacat
    31381 cgatgacaaa aactttcctg ttgatatcat taagacacag gttcattaca ccgagcacct
    31441 tgctttccac aggccgatgt atgctggtga tgtgcttaca ataaaagggc acattgcagc
    31501 cataatggcc caccgtgcag gaacggtagt cgttatccgc tttgacgcaa tagatcagga
    31561 tgggaacggt gttttcactg agcatattgg tggcttgatg cgcggcgttg agtgtactgg
    31621 caattcccgt ggcaaagaag atttgcctct ggtgccacca ttcagcggtg taccaggtgc
    31681 acaatgggag aaagttatac acattgaccg catgcagcca ttcatctatg atggttgtac
    31741 caacatacac ttcccaatcc acacatctgt tggctttgcc cgaattgttg ggctgccaga
    31801 tatcattctc cagggcacag caacgcttgc atttgctgca cgagaactta ctgataatga
    31861 agctgcgggc aatccacaac gtttgaagga actttcctgt agatttacgg gcatggtggt
    31921 accacctgca gacatccggg tactgctttt acagcgggag gagcatgcca gtgggactga
    31981 ccttttcttt gaagtacacg gaaaagaagg tcgcgttctt tcaaacggct atgcacgaat
    32041 aagtggttga actttctaaa aaatctgaca ctgcataaaa aaaatattga gttttttctc
    32101 aagaggcaaa gagtgagcag ctatgaaaag agttgctaaa aaaaattcac gggggcagtc
    32161 acgcaagccc ccttcgcctt tacctgaaaa actcaagcag cggctttatc ccgctgttct
    32221 tgaactcttt gctgataatg atttccacca cgtcaatatc cgtgaaattt accggaagtc
    32281 aggtattagc ccaagcacca tttaccgata ttttccatca aaagaggatt tgctatttac
    32341 cattctggat gaaaaaattt ccgagattgg cgttttagta cgagaacata tcaagggtat
    32401 tgaaagcaca aaggaaatcc tcagaaaaat tttctgggtg acaatggact actatgaccg
    32461 caatcctggt gttgctgtca cggccttcat taccgttccc atgcgtacct ggatgaagga
    32521 acgatcctat cgccgggatg atgcttcagc tattattgaa gaagtagtca aacgtgctcg
    32581 tgatcgtggt gatattgacc cctcaatacg gacttcgcag atccttgatc agtattatat
    32641 gtattgctac cgacagatac atttatggta tttcaatggc atgacaacaa ggcttgttga
    32701 ccgcattcct gctttttttg atattctgtg gaaaacactc agcccacctg agcatggatc
    32761 gtaaaaatgg acagtactaa aataccacag tataattgtc attttttata aaaattattt
    32821 aattgacttt tatacgggtt ttgttcattt acttcctttc atagagaaag cttgattccg
    32881 gtaactatcg cacatactgt aaggttttat aatgaaacaa tggctttaca aacatagtat
    32941 ttattgtata ctggcaatga tttttgttcc ggtagctatc gcccaatcat atcccaaaat
    33001 tagtgcatat atgccctctt atgcactgta tactgaatca gggcaaaaca aaaccactcg
    33061 ttacatcatt atacgaagat taaaatataa ttccgtacag gcttacgtat atataaatcc
    33121 agatacactg cacactggta tacttaaaaa tcctaaaggc actttgcaac aaaattctct
    33181 tcagaatatt attcaacaat gcgctgatac acaatatgca aagctgtatc aaaatgcatt
    33241 gcaaaaatca aaacgtatgt tcaacgcagg tataacccat gttgatacac atgggatgat
    33301 tttaagcttt gacctgtgtc catcaaaacg ctttcttgat actgattgta ttgaacagct
    33361 gatagcactg aacaaaattg taccactacc tgttgcaata tgcatcagcg ggaaatggat
    33421 gctcacccat ccagaagatc ttcaatggtt acagagttta gcttctgctc accagcttga
    33481 catcacctgg gtaaatcaca gctttactca tttttattcg cggaagctgc cagtgcgaaa
    33541 aaactttatg ctgaaagaag ataccagtgt ggcacgcgaa gtacttcaga acgagatctg
    33601 catgctggaa catgatattt ttccttcaat ttttttcaga tttcccgggc ttatttccaa
    33661 tcaaaaaatc tatgaaaagg taatggggta tggactcatc cctttaggtg cagatgcatg
    33721 gcttgcaaaa ggcaaaaggc ctgtgactgg cagtattatt ctggtacatc ccaatggtaa
    33781 tgaaaagaaa ggcacctaca ttttgatcca acttgcccgt tcagggttaa ttaatataac
    33841 aaatattgta tcattgacag cagcatgtac aaatagctca caaactgctt caatacataa
    33901 aaaacaggtt aagctgtaat aatacgaacc attgctacca gaaacttttg tcactatata
    33961 tttcttttgt ttatcactga ttcaatcttt ttctcaaacg aattcagtat atccttaatg
    34021 ggatatatgt cagacagcat tatatgaaac agtgaaatgc cttcaagaaa agctatggtt
    34081 gcaactgaca gcgttgttgc acttttttta gttgtttcct tgcctatgat agcaacaagt
    34141 tgctcaatcc attctttata tacatactgt agttttgttc gtactttttt atgatacagt
    34201 gctatttccc acacttcaat gaatatcttt gccagttctt cattcaatgt aattttattc
    34261 tttgcataat ccagcgccag tgacagcgct tcagcaaaat tgcgtgactc aagcttttgt
    34321 ttttgcgccc atcttttaaa atcatctaca tgcaatgcaa caatccagtc taaaaaatac
    34381 agtaatattt cttctttatt tttaaaatag taatgtagca tcccgtggtt aagtttggct
    34441 tcttttgcta tatcctttat tgttgttcga gcaaatggct ttcgtaacag gcagcgatat
    34501 aacgcctgtg ctatctctcc tctgcgtgtg tcttttaaaa tgcgtctgcc cattacacca
    34561 tgatttccat tttatatttt ttatcacttc cagatagtgt acacacaggc gcaagtgaca
    34621 tgagtgtaca cttttttttg caatactcca ttgttcaaaa ataattgcca gccagtcaat
    34681 attaatttat tgacaaaata aatattttat gttagtcatc caactaatat atactaaaag
    34741 gaggttcgta tggctttatg gaaagatacg ctcactgata atgataaaaa acttatcttt
    34801 tcactatttt cacggcacat ttctaaaggg caaaccaaga caatgcgtgc tgcacattta
    34861 gattttattg agacccggcg caaaggcatt ggttttattg atggactatt tgggcgaaag
    34921 atcattgatt gcttcacatc agctggctgc ttcaacgtgg ggagaggaaa tcctgaaatt
    34981 atcaaagcac ttaacgaagc gctgaacacg tatgatattg gcacaaacct tttgcaatca
    35041 aacgttaaaa aagaatttat aaaaaaatta gcatcaattg ctccaggaaa ttgcaatcag
    35101 gttatatttg cttctggtgg cggtgatgcg attgactgtg caataaagtt agctcgtggc
    35161 gcaacgggaa gaaagaaaat catagcaatg aaaaaagcat accatggcca cacggggtat
    35221 tcactttcag caaatggtaa ggattactat cgccaccttt ttgaaccact tgaacccaat
    35281 tttgtctttg tgcaatttgg cgacttagat gcaatacggc aggttgccgg cattgatact
    35341 gccgccatta tactggaacc agttcagggt gaaggtggca tctttgtggg tgatgactat
    35401 tacctgaaaa gtttacgaac tatatgtaac cagtttagca tcatgctcat ctttgatgaa
    35461 atccagacag gttttgggcg cacaggcagg atgtttgcca gccagcacag tggtgttgtg
    35521 cccgatatca tgacccttgc caaatctata agtggcggcc tttcccccaa tgctgtggtg
    35581 atgtaccgca acattccgct tcttacacag tatgttaaaa gcaatccgga atttcatcaa
    35641 tcaataaccg gtggaagtga cattgggtgt gccgtaggca ttgccgtgat cgactatatt
    35701 gtaaaaaacc gcctgtggga aaatgcagaa tcaatgggta acatcattat ggaaggactt
    35761 ctttccattc agcatgaaaa tcctgacatc ataaaagaaa tccggggcag aggacttatg
    35821 attggggttg aatatactca tgaatacatg ggagcgctta tggccgattc actggcacgg
    35881 catggcatct ttgctgtata ctcaggcaat cagccacagg taatgcgctt catggttccc
    35941 atcaccatac atgaacagga agtaaaaaag atgttagata ttatacgcaa agcgatagct
    36001 accatgagaa tgtatatgcc gcttaccaaa ccattatcaa aaattccact gctcaaaaaa
    36061 ctgttagacg atatccatgt acagatagct gtgtttgata ttatccgcta ctttgaggat
    36121 atccttggca taacaaaaag aaactacagt aaggaggcat aacatggatt tgcaatcatt
    36181 atttgaagga aaacgaaatg gtgaattttt ctatgataca aaaggcaagc gatatatcga
    36241 ctgctactgt ggtgctggca cgttcaacgt aggtcgacat gttcaggaac tatcgaacac
    36301 attacaagag caggcagata aaagcgatca gggtaatttt gttttaatat cacaggaaaa
    36361 ggcgctttta gcttcaaaac ttatttcatt cattccagcc aaactcaatg gcgtattctt
    36421 ttcagcaggc cgtggtgagg caatggactt tgcctgtaaa ctggctcgcg gatttaccgg
    36481 ccgaacacaa ctggtaaccg ttgatggtgg ctattacggt gaaaccggtt tttccgtatc
    36541 actgtcggat ggcgataaaa agaaaagttt tgggcagata cccgatattg tctccatacc
    36601 atttggtaat atacaggcac ttgaagaaag aataacagca aaaactgcgg ccgttatcac
    36661 cgaaactata caggtagaaa atgcatgccg cagtgccagt gaaggctttt ttaagattat
    36721 gcgcacagtg tgcaacagga caggcgcact tatgatactt gatgagacgc aaaccggcat
    36781 gggccgcacc ggcaaaaaat ttgcttttga acattatggt gtagaacctg acattcttgt
    36841 tattggtgaa gcactgggtg ctggcatatt ccccatagca gccacagcat ttaccaggaa
    36901 gatacagaag ttcatgaatc agcatccact tatccatttg tcaacgtttg gtggacatga
    36961 tcttggatgc agggttgctt atgaagcact tgaactatat gaaaaaacaa agccatggga
    37021 aaatgcaggg aatgttgggc aaaagctcct ggaagaactt gctgccttac aaaaacaata
    37081 tcctggaaaa attttatcag tacatggtag aggacttgtt attgctatta cagtacgcag
    37141 tgatgtgatg gcacagaaag ccgttacaaa acttgtgaaa gaaggtattt ttacagcaac
    37201 tggcaaagtg gctcgcgatg cagtcatcat acgaccggcg ttaacaattt ccgaagagac
    37261 agcaaagaca attgcacgga gtatacgtag agtatttaaa acattataga tgaaacatag
    37321 tttttattgt taaatacata ttgcgccaga gtggtctttc ctgactggcg aggacctgtg
    37381 attgctacca cagggaactg ttttgataat tttttaatgt ttgttcagct tcacgtttta
    37441 tcatatggtt tcttttgtaa cactatggat aggtaataat atatgcacat cagttattat
    37501 tgaatactat ataacttgca aattgttaat acaaattaca ctttgtaagt tacaatattg
    37561 aactatggga ttatataaga tcggtattgt aatatgttat attttacaaa aaatttttgg
    37621 gaagttattg actataaatt ccgtataaag taatagcaag aattcatgaa gataaacacc
    37681 atttaatttt gcattttttc ttgacgaaat ttgagtatga tgtactcttg taaaaaataa
    37741 aaaaagatag tatttttttg tgacataaat gattttttat agtatatttt atgaaaagaa
    37801 catatgttaa acgagttgcc ttcctgctga aagagttttt atataacctt ttgtagatta
    37861 tgatatatgt attaaataat tacagcaaaa ataaatcatc atgggaaaat cctcttagca
    37921 ataaagtaat aaggtactgt tcaatatcgt taacataatg ttatttgtta ttttaaacta
    37981 ttaccataca ttttcgtgta aattttacac aatccatacg gtttaaccag ttattgtata
    38041 gcagagataa ttcttgtttt taaagaagct gttcatataa attccaaact taaatattct
    38101 aaaggtgtgc tatgacaaaa gtatcaaatg aaaccgttga aaagaacaac aataataaca
    38161 gaaaaaacgg gaacggaaac ggagattatg caaaaaaagg caatggaacc attcaggaag
    38221 gccgtttaga tttagctgaa ctcaaatcaa aaaccatcag ttcccttatg gaaattgcac
    38281 gttccatggg aatagaggga gtttcaggac ttaaaaaaca ggatcttata ttcgaaatat
    38341 taaaagctca aactgagcgc agcggtttaa tcttctcaag cggtgtactg gaagttctta
    38401 acgatggata cgggttcctt cgctcaccca actacagtta tctgcccggc cctgatgata
    38461 tctatgtatc gccttcacag atacggcttt ttggcttacg gacaggggat accgttgcag
    38521 ggcaaattcg gccacccaag gataatgagc gcttttttgc cctccttcgt attgaagcgg
    38581 tcaattatga aaacccagaa aaactgcaaa accgtgtatt atttgataac ctcacaccac
    38641 tgtatcctga aacacgcata aacttggaaa catcacccga tgaaatagat atgcgcattc
    38701 ttaacataat tgcacccata gggatgggac agcgcgcact gattgttgct cctccccgta
    38761 ccggtaaaac cataatctta cagaagatag ctaatgctat caccacaaat caccctgata
    38821 ttatactaat agtattgctc attgatgagc ggcccgaaga agttaccgat atggagcgtt
    38881 cggttaaggg tgaggttatt tcatcaacat ttgatgaacc ggcttcccgc catgttcagg
    38941 taagcgaaat ggtacttgaa aaagcaaaaa ggcttgtaga gcataaaaaa gatgtcgtta
    39001 ttcttcttga ttcaatcacc cgtctggcac gcgcatataa tcaggtggtg ccaacaagtg
    39061 gtaagatttt atccgggggt gttgattcaa acgcactgca taagccaaaa cgtttctttg
    39121 gagcagcccg aaacattgaa ggtggtggga gccttaccat tatagcaacc gccctcattg
    39181 aaacaggaag ccgcatggat gaagttatct ttgaagagtt caaaggcaca ggaaattgtg
    39241 aaatcgtact tgacagaaaa ttagccgata gaagaatatt ccccgcaatt gatatcaaca
    39301 agtcaggcac ccgaaaagaa gagcttctgg ttgatgcaga agagcttaat aaaatatgga
    39361 ttttacggaa ggtcctgtct tcaatgagtt caactgaagc aatggaattt ttaatcgaaa
    39421 aaatgcgtgg aacaaagaat aataaagcat ttttcaaagc aatgcaggga taattgtgat
    39481 atatttattt ctataaggat aaggagatag ttatgaaaaa aggaatacat cctgaatatt
    39541 atgataccgt gataaaatgc gcatgcggta atgagatccc aacacgttca acagttaaag
    39601 atcttcatgt tgaaatatgc tcaaactgtc atccgtttta taccaagaag cagaagattg
    39661 tagacacaac cggacgtgta gaaaaattta agaagaaata caatatcaag taatttttag
    39721 taacatttag tactgattat ttacgatata gtatgagtac gtaaacatgc gtcaccttga
    39781 aaaaggtggc gaagtttttt tataaagtat accgtatttc ttcttattac catatacgaa
    39841 caagctttta atgcattatt aactatttat ctaacccata aaaagcaagt ttacgttgta
    39901 taataattac acagtatagc gtggtactgt ataattttta tacatttgtt acttttttaa
    39961 aaattttatg ggtaaatcat tgacgacttg agatactatc tgtatatttg tgtaagtatt
    40021 ttagcagcta attattttat tttttactat tttagctgta aaatctgatt tttcaaatta
    40081 ttttaataaa cattttaaaa aattactcag aaatagctgg ttttatagta tttataaaga
    40141 attattttaa atatttataa gtttacgaaa attttggcac acatattgct ttatattatt
    40201 ggtgaagcga aattcatgga gaggaggcag ccatgaaaaa atacaatgac tttgaagatt
    40261 tgtcaatcaa tcagtacttt aacgcaatta atcaggtaga acttcttaca cccgaagaag
    40321 agattgaact tgcaaagaaa aaatgcaaag gtgatgagaa ggctaaagaa gctctgatta
    40381 atgccaattt gcggcttgtg gtaaagattg caaaaaatta caccaattta gaatcctcat
    40441 tgattgatct tatccaggaa ggtaatctgg gattgatcag agcagcagag aagtttgatt
    40501 acaagatggg ctgccgattc tcaacctatg cttcatactg gattaaacac tatataacac
    40561 gatttattgc aaagcgaagc cgtactattc gtgttccaat acgaaaaggt gatcttttca
    40621 aaaagattaa aagggctcag gatgagctta ccaatgaatt aggtaaagag ccttcatata
    40681 aagatattgc cgatatgtta ggtattgatg aacgtagtgt aagcgatatc ttagaggtgt
    40741 tccagccaac agtatcactg gagtatccac ttaatgatga agagttcaat ctttacgaag
    40801 tggtaagcga tgaaaagttt ctgtcgcctg ataccagcat ttcacgccgc gatctcaaga
    40861 aagagttaga gatagcaatg gaatcgctga tggaaaatga acggcttata ttaacaaaac
    40921 ggtttggcct tgatggcaat gagccggtga cattgaaaga tgttggtaac gaatttggaa
    40981 tctctgcaga aactgtacgc cagatagaag cccgtgcaat gcagaagata aaacagcgat
    41041 ttgcatactt agcagattac attgaataga atatacgttg tgttgttaaa aaaccctgct
    41101 gaatgcaggg ttttttattt atttttgcca caaaaaaata agattgtatt atcccatacc
    41161 ctatcccttt ctccatgagt aaggcaagaa aagtaatttt atctaaattt ttatttacaa
    41221 tataactgtt ttattatatt aacatccgta gtattattat catctttttc cagggggctt
    41281 tatgaaaaat tataaaaagc tgattatatt ttccattctt ctggtggtgt gtattgccat
    41341 tatagcgtat ttaactgatg ggtatcaaga aaatgataat accagtatgc ttttcttcaa
    41401 tggcaatatc ataactgttg atagtaccac atccaaacct gaagcagttt ttataaaggg
    41461 aagcaggatc tattttaccg gtacattgca ggatgcacgc aaactaacag atgccagtac
    41521 tataacaata aatcttaaag gacgcacact tattcctggt tttaacgata atcacacaca
    41581 tacattagct gctggctcat tttattctga agcaatattg tggaacaaaa cctgcgaaga
    41641 aatagcggtt attatatcac aggaagcaaa aacaaaaaaa cctggtgaac ttatctctgg
    41701 taattcatgg gattatacca cgtgcccaaa gcctcataag tcacttcttg ataaagcagc
    41761 tcccaacaat ccggtatttt taacgcaata ttccggacat gctgcatggg ttaactcacg
    41821 gatgctggaa gaaatgggta tagacaaaaa cacgcctgac cccaaaggtg gtcagattgt
    41881 acgtgatgat aagggagaac caacagggat tctgcgtgat actgctatgg gaacatctga
    41941 atacggtaaa ttcattaaac agatactatc gccaagctta cataaaaaac tcattactaa
    42001 ggctctgacc ctttacaaag aagctggtat cacatcggtt caggacaaca cgtgggaacc
    42061 cttcacagtg cgattactaa aaaaatatcg taattcacag gaactatcga ccagatttac
    42121 ctgctggccc atgggcaact cacccctcta ctatgccttc acctggtttg catcatttga
    42181 taaaaacgat tactgggtca ggaaaatttt ttatcagcct cttctgggca tctattatgc
    42241 tgtaacccgt aaaagcaaag acggaaccat ggggcctctt aacaaagatg aatgtttttc
    42301 tccgtatgag gcactgtact gctacacaat ggggtcagcg tatgctgaat ttatggaaaa
    42361 cgagaaaggc tccattacta aaggtaaact ggctgatttc gttgttcttt cggataacct
    42421 tttaacggtg ccagttcata ctatcaagga tatcaaggta ttgatgacaa taacaggtgg
    42481 aaggatagtg tacaaacata atgatttttg agtattgaat aaataaggtg gggttttgaa
    42541 aaaaccccac cccttactat ttatagtacc cctccagatt gattaattcc tgaagcatct
    42601 gtttacaaca atggggtttt gaaaaaaccc cattcc
//
LOCUS       DAORYL010000015        41717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_745_length_41717_cov_12.951707, whole genome shotgun sequence.
ACCESSION   DAORYL010000015 DAORYL010000000
VERSION     DAORYL010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 41717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41717
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_745_length_41717_cov_12.951707"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PLV81_04745"
     CDS             <1..485
                     /locus_tag="PLV81_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09439.1"
                     /translation="IILLLSSIFYFSIILYVHRNRYCMSLFLLTFFMYYMYLYYVKVI
                     EMIQPVKHNPSIPVIMQHKARVGILETQNDVLAPMKRFKKELPVPQLGQQPNLSLEKV
                     RIRQKEFYSHRGQVYEKLQNTSAAIASYQKASYYEPSDSNLYGQIKRLKIQSLTNLKA
                     "
     gene            645..1862
                     /locus_tag="PLV81_04750"
     CDS             645..1862
                     /locus_tag="PLV81_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOM09440.1"
                     /translation="MVQEQFKVVNYLSNSFIVVEGKRNADNFYIIRQGKVKLVKENPI
                     AQETNPLLGPGDFFGVIPCMSGHAHIESAVALTDVSLISVQRDQFGILIQKNPAVAMK
                     IIRFFSRKLREFDQAITRLTFANAVEEDPEHLFKIGEYYLKKKNLPHAAYALQRYIQH
                     CPNGTNRDKAIAHLKSINAPLKVPENPQKNSLTRLYKDNQMIFCENEPGDELYIIQGG
                     KVKITKIVDEEVLLAVLKPGDIFGEMALLENRPRSASAITFGDTTLMAINRQNFETMV
                     QTQPQLATRLIQLLSERIWTAYRQLENLMIRDPLGRMYDTLLIQVEKQKVRIAPKESF
                     TFDFGVKELLNMVGIPQDKGDHLVVELLEDKNITLDEGKLICTNLEELEKTVNFYKKK
                     SALERKREASKSS"
     gene            1840..3936
                     /locus_tag="PLV81_04755"
     CDS             1840..3936
                     /locus_tag="PLV81_04755"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOM09441.1"
                     /translation="MKPAKVVKYSAIILILIIMLLAAFAAAFVYLFPKETLKSIIVQT
                     LGDSLNRPVTIGAIDYSIRGIQITDLYIYDTDKEKPYIAHSKDAAIRFQLVPLLQKQF
                     IINYISLNDAIIHILYYTENKITTSNLEKLLKELLSKEKSTITTQIKLISLNNTTVIL
                     ENPPQNLKPLEGKYSIDTHVELHDDILELKNLSINLPQNRGKISGDCSITITSNYKIQ
                     GDFTLRDCDLLWVYNWGKNLATILPYRVFSGTIDNLLITKSAVQGTVTGSCKLSNNAM
                     LFLSNAFTNVNLNKKTVTVANAKGKIENSTFVLNQLLFAFSGKIQNIKVSDASINTKE
                     IAPLLGKIDISDIAGELKGNFEYKDNSIDADVTLSNLSIGKKGTILSLQETNLLIKNN
                     QFKKDGIQATVYSVPCVLSVATVDSSFEKFTVNIEADEIDLNKIISRQPQKPEQPEKT
                     EKEIHIPYELSGKCIIQKLYKDNLVIEKINCNYTMSKNIIALPTFSAKVFGGDITGRS
                     VITLSSENPQITLVAKFSNARLQNISSYFQEYENRLFGNISGQLQVTIVPKDPVIDRI
                     TGKVEVSVENGKLANTGLQNGLSIWLSDLKYKLSNLEFQKIYGNFNLSGPAITINSFI
                     FNAPDIRLKLNGQYNRHEESDVAISLEFSSNFIQDLPNPALLQLAKYKKGRWYIIPFR
                     AQGKDIFEGKNIKQVQ"
     gene            complement(3938..4432)
                     /gene="leuD"
                     /locus_tag="PLV81_04760"
     CDS             complement(3938..4432)
                     /gene="leuD"
                     /locus_tag="PLV81_04760"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOM09442.1"
                     /translation="MKAHGKVWKFGNDINTDEIIPARYLNTSDPDELAQHCMEDADPD
                     FMKKARPGDIIVAGKNFGCGSSREHAPIAIKAAKISCVIAQSFARIFYRNSVNIGLPI
                     IESEEAAKDIEQGDEIEIDFDSGVIKNITKNKEYRAKPFPPFMQDIIAKGGLMAKVKA
                     ELKK"
     gene            complement(4473..5732)
                     /gene="leuC"
                     /locus_tag="PLV81_04765"
     CDS             complement(4473..5732)
                     /gene="leuC"
                     /locus_tag="PLV81_04765"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOM09443.1"
                     /translation="MGMTISEKILAAHAGKERVEPGELIEAKVDLVLGNDITAPIAIK
                     EFEDLGVSKVFDNEKIALIPDHFTPNKDIKSAQQVKILREFAKKYNIVHFYDVGRVGV
                     EHALLPELGLVRPGMCIIGADSHTCTYGAFGAFSTGVGSTDMAAAMATGKTWFKVPES
                     IKFVVYGKLQPYVSAKDIILHIIGSIGVDGALYKSMEFTGDTIAELSMEGRMTMANMA
                     IEAGAKNGIFAPDGKTVEYIKSRTGLPYTLYYSDDDARYVEIKEYDASKIEPTVAFPH
                     LPENAKPAREAGIPIDQVVIGSCTNGRIEDLRVAAKILKGRKVHKDVRLLIIPATQEV
                     YRKALEEGLIDIFIEAEAAVSAPTCGPCLGGHMGILAEGERCVATTNRNFVGRMGHPK
                     SEVYLASPAVAAASAILGRIAHPDELR"
     gene            complement(6438..7634)
                     /locus_tag="PLV81_04770"
     CDS             complement(6438..7634)
                     /locus_tag="PLV81_04770"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOM09444.1"
                     /translation="MADNDVVIVSACRTPIGSFGQSLKDVKAYELAALVMKEALRRAN
                     VEGSALSDVIFGNCLMGSDEANVARTAAIKAGIPVEVPATSVQRQCASGMTAVVFGSQ
                     QLMVGDSDIVLAGGVESMSNAPYVLWKARWGLRLTHSELTDAMWELLHSGSGLLGEPY
                     IMGQTAENLAEKYSISREEQDQVAYESHKKAAKAIDSGRFNDEIVPVPIPQKKGEPKM
                     FEKDEHVRRDVTLESLSALKPAFKKNGTVTAGNASGINDGAAALVLTTAKRASELGLK
                     PLARIAGNAFAGVEPHLMGYGPVPAVQKLLKKTGKKLDDIDLIELNEAFAAQYLACEK
                     GLGLDRSKVNVNGSGIALGHPVGCTGARIIVSLIYEMKKRNAKWGIATLCVGGGMGGA
                     VLVENV"
     gene            complement(7852..8196)
                     /locus_tag="PLV81_04775"
     CDS             complement(7852..8196)
                     /locus_tag="PLV81_04775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOM09445.1"
                     /translation="MLNVSVNQLADVVVIDLEGEFDIDVIRDVEKAWNDALEKKPRVI
                     GFNCKELKFIDSSALGTLVKFMNSATNRKISLALFNVAPSVITIFKTSRLDKFFSINS
                     KEEFENKFLPVK"
     gene            8381..8746
                     /locus_tag="PLV81_04780"
     CDS             8381..8746
                     /locus_tag="PLV81_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09446.1"
                     /translation="MRFALLLKVLALILRIASKRNEDFKRFIGTVSVKIAIKTKNNKG
                     RTFIFNNGSVSSTRSLKQYDAALVFSDAKTGFTVLKEGTQEASFYAAATGTLYVEGMA
                     FFIQWFNDAVTIAMKKNKK"
     gene            8759..>10348
                     /locus_tag="PLV81_04785"
     CDS             8759..>10348
                     /locus_tag="PLV81_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOM09447.1"
                     /translation="MSINGGHLIGKYLAKQGIQFAYGISGGHIEALLDGLQQYGIRSI
                     DVRHEQAAVMMAHATAIYTGTPGVCFLTAGPGFTNGITGIANACLDNAPVVVLCGRHP
                     LRDDHKGALQEMNQVDVVKPLVKWCATCYDTKRIPEYLDMAFRHATGGRPGPVFLELP
                     PDVLGKAVDEFDFTPSVDEFKAVPDSSSLYKAAEIINSAKKPLFIGGSGIGYSNCTLE
                     LQAFIEKTGIPFILLNNGRGVLPDNHPLSIWNTGNVGLMMTMSQADVIVAAGIRFNWL
                     FQSGAVIPPTTKVVRIDIEPTEINRNRVADVGLLGDTGAALKALLPLVEQRNHQQWLD
                     MLTQAGYAFIDSEIKLRQNPSDPIHPIRLVAKAQEVFGTDALYVVDGGDTTYFGLVGF
                     QSRHKAGVLSQSAGLLGCLGAGIPFAIAAKLIHPDKQVVVLSGDGSFGFNGFEFDTAV
                     RHNIPFVCIVNNDCAWGMIKHSQELSIGKERVTCAELGIRRYEKVVEGMGGHGEFVEK
                     DEAIAPALIRAKESGKPACVNV"
     assembly_gap    10349..10448
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10449..10850
                     /locus_tag="PLV81_04790"
     CDS             <10449..10850
                     /locus_tag="PLV81_04790"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOM09448.1"
                     /translation="MKRIFEIHELSATLTPVFTKHNIIFAYLFGSIVNNESTPTSDID
                     IAVYCKGVMDDSVFEKKLSLHLDICRTLKIDNVDIIVLNKTTNLMLIDEVIRNGILLY
                     DTDTSLREKYEYFMIHKAIDFKTQRLYTMGI"
     gene            10850..11272
                     /locus_tag="PLV81_04795"
     CDS             10850..11272
                     /locus_tag="PLV81_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOM09449.1"
                     /translation="MERIIQKIGKIHEYLTIIYTIKDDCINKIYTDPIYKGALLHYLY
                     LLSDSCISLAELVIKYKKLRVPQTYHEAIDILGENNILNPDFAYSFAKIAGFRNYLAH
                     DYDQLDTAIICNEILKRTKDIEEYLVQIKNSLGIKYYK"
     gene            11310..12509
                     /locus_tag="PLV81_04800"
     CDS             11310..12509
                     /locus_tag="PLV81_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOM09450.1"
                     /translation="MKALQFNVTIPQWVALKALGACTKSVFYKSPIGTLKLVEIPEPE
                     PINNNWAIVKTTYCGFCGSDFNLILLHDSPMASPFTSFPCVIGHEVCGTIEEPGNTGF
                     KKGQRVVINPMLSCAVRQLPLCASCKKGRPANCENFANGTISPGMFTGICKDVNGGFA
                     EYLPAHRAQLFAIPGSISDKAAVLTEPLSVALQAIYDNMPQHSDTVCIIGCGVIGLML
                     VRALRALNCKATIAVIEPSEFHRQKALSLGANAIVQGDLLEHAATFTSGKVYTPMFGP
                     KILQGGFDRVFDTVGSSKTFSLAINIARAGGVVSLVGITSKLSYDPTPLWLKLLTVKG
                     VYGYGFIREGSKTKHIFAKALELMKKMPDIETMVTHTFSIDQYKEMIEVNSNKNAHKA
                     IKTAITF"
     gene            complement(12506..15217)
                     /locus_tag="PLV81_04805"
     CDS             complement(12506..15217)
                     /locus_tag="PLV81_04805"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOM09451.1"
                     /translation="MKEHKDIIKRFFGSNVALHCIREAIRYAAIVRIATAYFEPSGYN
                     LLRGVLKDKEIRLLIGRNEDTQEKIETIIDKFIEDLASASIEDKTAFIQELCEALGAG
                     YFTVNVSNQPATFTTLAPKFLYQHAKLYIADTHSAVVTSANLTYSGLVQSREAGIVVT
                     NEDDVLFFVEKFDEYFSQAQPLAQELLEKLQELLALYPPYYIYMRSLLLLYDMPEDEL
                     PDKLPPLAGYQKPIVSRLLRIIEEHSGAWLIASTGLGKTVMAAHIAAYLCMSKRINAA
                     LVLCPAGLKRMWHRILRAARVSSEEFSYYILSLDDFKKSIDVAHLLSELAHIDENTLI
                     ILDESHHLRNIGSADNIRLSHQRIMDAVHERKAKILLMTATPYSTGVDDINSQLVLLP
                     HKEVDGLFGKCVQPWCIESPSELSELLPVVVLTAPTVVKYYSSIDEAGNRYVLFSHEE
                     KRYFPHKLHFQNIEYTNQGDSILHQLLQSKLLYRKVNDTSNTLFHDTAGVRDPLWEAQ
                     IVHQACSSLKQLDELLDKLSREDGFDKIRFHNQERLSSFAVDSRVRLQPLLYERDEKI
                     QKLANLIERFNHEKVVVFCHYRETARYIKDALQRILPHVVVESTVDADTNKIESIIQR
                     FAPIANKADYVDDENNANGKEIQVLVATGAMSEGFNFQDARILVNFDLPWTVLTLAQR
                     MGRILRPWFEPRDVYVFNFIPSTMGSDIVYHASRWKNRLLTRNKEISSFADIPVLVAD
                     GGKDFEMINLAKSIALFGDHDLNLDEVLEFIETADNLHTSGFIDDIAQLDESVKREVL
                     RLPSGIRSIKKSNLKEPIVIYILFKCKNRYYPVLFDGKEKILYDSEHMDEIMHIIRSL
                     KDEEPFYNAEIVQSIDSVLVKAKNEWVVKHQFHPYEVMIDCYCVLLS"
     gene            15547..16521
                     /locus_tag="PLV81_04810"
     CDS             15547..16521
                     /locus_tag="PLV81_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOM09452.1"
                     /translation="MKKVLIVFVAIIIALIAIDAYQSHITYTYTPKKLPATFDEYYQM
                     KLKESKEKGARPHNEEKLLKFADKTKIAMLYIHGYGASRGEGEYVIDTIAKKLKYNTY
                     YLRLPGHGTTMDDHKSTEYYQLLDTAIEAAHMTKLLGEKLVIIGTSMGGTIATYIAAE
                     HPDIPDAVILVSPFYRFANPVGNALLFRPFFKTVLLFAKYRERHDPYDDPNDNWTMYW
                     YAKNYWASLHSLLDMSDLIARDDVYRKVSCPVLLLYYYKDNEHKDGSAQVEAMLEAYD
                     IFNNGKPNPLSRKIAVEDGDHVLLSKYVKSDWGKAEKAITDFLNDIAQ"
     gene            16872..>17056
                     /locus_tag="PLV81_04815"
     CDS             16872..>17056
                     /locus_tag="PLV81_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09453.1"
                     /translation="MSTIKRIFILFMFCVTSFFFVTGKSYSEGKNLYFPIGLTYNSTY
                     WWRGVEINGKGAGVLWP"
     assembly_gap    17057..17156
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17272..17907
                     /locus_tag="PLV81_04820"
     CDS             17272..17907
                     /locus_tag="PLV81_04820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOM09454.1"
                     /translation="MDNYLKVSTFKRLMFNVKTRLYWELSYKKKFASIAGNPIVWGLW
                     NIDIYGPNIHLGKNVVFLAANGSKTALTTVRLAGHEGCIDIGNNVLVMNGVRISSASH
                     IVIGDDCMLANFCYLTDADWHDIHDRTNPVGKTAPIILEKGVWIGDSAIICKGVRIGQ
                     NSVVGAGAVVTKDVPPNVVVAGNPARIVKEIDPDKVVTHSTLYHKVGAPRI"
     gene            17904..19139
                     /locus_tag="PLV81_04825"
     CDS             17904..19139
                     /locus_tag="PLV81_04825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOM09455.1"
                     /translation="MRICLLCYRANPFCGGQGIYLKYVAEALARQGHEVHAIVGPPYP
                     HHMDHVTIHYIENNQYYIKKGVECITYDNPFDIFHPINAYEYIHSRLGAFPEISGFSY
                     RAFFMVRKLHEKLHFDVIHDNQCIGYGLLFMKALGIPVVATIHHPLTIDLENVLERAS
                     SFKNRMKGVMFYPILMQQIVSKRLDHIITVSEDSKHRITKDFGVPPHKQSVVYNGLDT
                     SIFRKLPNIKKKHNKLLFVGNVEDGKKGFVYLLKALTLIQSDATLTVIDGGAPHRRIT
                     QKLVDTLGMRNKVEFVGAASTDELVHHYNEATIAIVPSVYEGFGFPAAEAMACGTPVI
                     ASDGGALREVVGDAGIVVSARDEVALANAIDNLLQNKKLLQELSHKGIKRVQTHFNWD
                     NAALQMAAIYEKVISSYYR"
     gene            complement(19130..19921)
                     /locus_tag="PLV81_04830"
     CDS             complement(19130..19921)
                     /locus_tag="PLV81_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09456.1"
                     /translation="MNVKSKNNCYIVVILSLVVLYGCTSVPVKETEKKETSATLLLEE
                     IIKLNATLPHTVIASLRIENISKEGFKSSAKLLYNVDARKAKMSIVDSVFGTQLSVVV
                     RDNDTLYIYQPIANNLLVFDYNKFPVYSFLPFNIDVELIDNLLIGKIPLLKNYYSVKA
                     LEADSEQFILLENDTMHQTISMKDNVPSKILFVVKSTNSRYEIYFLKPKIDNNMFTCK
                     ALRFVDVNGKTTFEVTMQQYVINEAVPDTVAELKLPKNTKITDYR"
     gene            complement(19929..21785)
                     /locus_tag="PLV81_04835"
     CDS             complement(19929..21785)
                     /locus_tag="PLV81_04835"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM09457.1"
                     /translation="MKKFLPLLMIIAIACSSLPDTKTETIARPVYKETSFELYSKALL
                     LKNEKKYTEAIKLFEEASKEGTQLDAIYYQIAECYYYQYDYAKAIEYASKSIAVNENW
                     DKPYVLQYSVYMNLNQADEGVTVLEKLLQKRPELYRVRFNVATVYYSQLKQNVRARYH
                     FLKVIEQSECESIESYYKEQSYYYLGHIYYSFGEFKKAYHYFLKAYNENPENYSMLYI
                     LGLLNLEKGDIKQAISYIIQYLERYPNDLKAILHAGRIYYIYEDMKALPVLRKAINHR
                     SSEGLLARMMYNELLHNDDAARELAQKLNRMNTSLDMPYIALARIAMRKHDTATAAAN
                     FYTAGVMLYQKKDYEAATIMFTLALTNDPSIYEAYLYLGQLYEDTNQLNRAIYYYTIA
                     YSMKKENDVLLRIGYLYALLKDYTKAFEYMNAAQNQDKENPDAYFFKGLVYMQKNNYP
                     DAEDMLRKAVDLKKSETYYFYLASALDKQKRVQDTIAVLQEACNNEQKSARICNYLGY
                     LYADNNIRLDESLMLINRALESEPTNGAYLDSLGWVYFKMGKYHDALPLLLEAAQELE
                     LEGYPDAVVYEHIGDAYNALQLPDKALEYWNKAYKLDPKPEVQKKIDTHKGK"
     gene            22138..22836
                     /locus_tag="PLV81_04840"
     CDS             22138..22836
                     /locus_tag="PLV81_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOM09458.1"
                     /translation="MLLGIDPLYWMMMAPVLLISLWASFRVKSAFNKYSQIPSRSGHT
                     GADVARMILQQNGLSHIPVEETTGFLSDHYDPIKKVVRLSPEVYNSNSLAAIGVAAHE
                     TGHAIQHAKLYRPLVLRNAIAPTASIGSNLSWIIIFAGFAMGSLGLVKLGILLFSLVV
                     FFQLITLPVEFNASSRAKEILQGYGIVSSGELAGVSKVLSAAAMTYVAAAASAIMTLL
                     YFLIRAGLLGGRDE"
     gene            23524..25662
                     /locus_tag="PLV81_04845"
     CDS             23524..25662
                     /locus_tag="PLV81_04845"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOM09459.1"
                     /translation="MMKVPGSVQVLSILIVCLWITGCDTGDPFIDIHSDWKLYSGDNY
                     SIAQPEFDDSSLQSINLPGKLSPKKSRQYLWLRKSVVIPDSFKNYDIAFVLGKVWDVE
                     QTYFNGYKIGSAGSEYPNFHSEWNSFRYYVIPLHIVKYNQPNIIAIRVFSNQNAEYNG
                     VPLITTLANAKVINFYRSLLAEYIPLATSFLTFLLAIIALYFYFKNKDVLTLYFAIVS
                     LLWCISAMHFYLPHYWIMDFNTQDKIYYALTAVIAIVVYFFFENVTDSLNRTLRIIIT
                     IAAACMIILSLSATEHDPVTGWRFQVIGSFGLIVQILWGALIIKGIRNNKKEAQTLLI
                     PYIFMMICVGHDSLAVSGIFYTNFFWINLGYPALIIGIGAILSQRSAKLAQELLESKQ
                     YIEKKNADLIQIVQKINQSIGELQHVAKEVESSARTLQNSMHDQTTSIEQTSAALEEF
                     STTVDIIASNSKHQDTILNKNRDLLMELIKGIGHITDAAKTAVKLSYKSQGQTAVTKE
                     HLSEALKGIEKIKNYSDTILTITETINDIAEKTNLLSLNASIEAARAGEYGRGFAVVA
                     DEIGKLADRSLEQSKNIQAILSDIVTDIENQTILMGTVVYSNEDVERSVYMVNHAIDT
                     ILDFCISQEQLTKSIEENMALVLQGSSQITSATQEEKITIGEISSTIQQLVTITNAVY
                     ENTEMLAKTLTKILSQIDMLQSVVQTKNNS"
     gene            25680..26207
                     /locus_tag="PLV81_04850"
     CDS             25680..26207
                     /locus_tag="PLV81_04850"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOM09460.1"
                     /translation="MNYRHYKKTTLLFFILLVAIFLSLIIFFKAFYPCPLSTIPSKTT
                     EHDFLQYFDHRIKVLMGKYEIPGVNIAIIRQGKILWANAYGFADKEKTIPMKLETTCR
                     VESISKSVTAWGILKLVERGAISLDEHIIKHLKTWKFPPSAFHPEAITIRQLLSHSAG
                     LPLGTIGFMYFFIPE"
     regulatory      26292..26352
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            26413..26574
                     /locus_tag="PLV81_04855"
     CDS             26413..26574
                     /locus_tag="PLV81_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09461.1"
                     /translation="MLTMFVVASVWLGLAVVSTLIANTYFLPEHLLEAPMADEEIPQL
                     DGLNGANNT"
     gene            26730..28061
                     /locus_tag="PLV81_04860"
     CDS             26730..28061
                     /locus_tag="PLV81_04860"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOM09462.1"
                     /translation="MCDTIVATSKATADGSVILAKNSDREANEVQLLKYIARRNWGNE
                     TAVQCTYISIPQVKSTHEILISTPFWMWGCEMGANEYGLAIGNEAVFTKEPYEKTGLL
                     GMDIMRLALERCKTAYDALMLSTELIERYGQGGNAGMEHKLYYHNSFIFADPNEAYVL
                     ETANKHWVAIKVDGIRSISNGLTIEEEYHYSSKNIEDYARKKGYLKKGKTFNFKECFS
                     DWFYTYFSRCAARQSRTTRCAMQHSGSITPQSMFAFLRDHGSDHEYISPDKTDMGTVC
                     MHASLLPTRPSQSVAAFVAHLRKDTPVFWATGSSGTCTSVFMPFYLIGKEIDYFPANE
                     TSTYSPDVYWWMHEKIHRQAIFKWDYFQRTIAPAIHTLEDEFIKKENELYKKKNTSSQ
                     KYFDFSKQCVEKVIELHDRWDKELKAAPASPTKLSFKLFWNGQTKKAMIPL"
     gene            complement(28062..28727)
                     /locus_tag="PLV81_04865"
     CDS             complement(28062..28727)
                     /locus_tag="PLV81_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOM09463.1"
                     /translation="MKVCDGVYAYIWRGVFENNCNTFYLGDPFNMFIDPGLAKYVDMR
                     FADMKKDGIDTEKIAYIVATHSHPDHFEGCQQFMGKGPKVAMHKEEIAFLNEIGPQFF
                     AMFGMKFPQFTFDIELEEGPWKIGNEVLQVYKTPGHSPGSVCLYWESKKVLVCGDLIF
                     KESVGRTDFPGGSSALLKESIERMKELDIEYLLPGHMDIVVGKSNVKKNFELIEKYIF
                     PML"
     gene            complement(28731..30803)
                     /gene="glyS"
                     /locus_tag="PLV81_04870"
     CDS             complement(28731..30803)
                     /gene="glyS"
                     /locus_tag="PLV81_04870"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOM09464.1"
                     /translation="MANCNFVCEIGTEEIPAGYIPSAIEQVEKIVTEGLQSYRIAFGK
                     CTVYATPRRVAILVYDMADSQSEDYEELKGPSVKAAYDDRGNPTRALTGFLEGNKIKL
                     EDTYIQKTSKGDYIYAKRKVEAQPATTIIPVIIEKIINELNFPKRMRWNVKRVSFPRP
                     VNYICVLFNDAVVPYSIDGIDFDNKVRGHYIQYNRMIPLSRCDEYIPTLENNGVIVDH
                     NKRKAIIHEQLQNAAKQLGGVLVEDDELLTTVTFLTEMPYVVIGSFDEGFLSIPDIVL
                     ITEMKEHQKYFAVRDSKGNLLPKFLVISNNPPTEHIVKGNQRVIRARFSDARFFFEED
                     RKVPLAGRVDMLKSILFHKELGSIYDKVERMQTIAKVICNELSLNTQITQKIQRAVML
                     SKTDLTTALVFEFTSLQGQIGKIYALLDGEDEEVAQAIDFQYRPRFQGDVLPEHIVSI
                     VVSLAEKIDNIFGSFSVGNIPKGSADPYALRRQAAAIVDMLVERTIHLDLANVFREVS
                     SQYSNGDKHVEPILEFINGRAKTFLYEKQFAFDEVNACLAVGCYDYYELFLRAQSIHE
                     FRKDKRFGDLLIAFKRMNNIVSAFVKKNPGYVFSFNPDALIIDEEKRLYSFFNNKEKT
                     IKEYIAENKYRELFLLLIEGKEIIDTYFDKVMVMDANTSIRDNRLGMLHYILTLFTTL
                     IDFSQIQE"
     gene            complement(30840..31850)
                     /locus_tag="PLV81_04875"
     CDS             complement(30840..31850)
                     /locus_tag="PLV81_04875"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOM09465.1"
                     /translation="MKHWQLHSYSFGSLSITVLSAEIIIIFRGTKTTIIKSIPFKKIK
                     SIALVCGTIVIGVILFTGYVRSHHVFNSEAPWYDEIKKKAIASKDFEEPDKKKPLKIV
                     QHTVKSGETLSEIAQMYGVSMDTICGCNRLESYDLVHVGQVLKIPNKDGLLVSVQRGQ
                     TLPALAKKYNVAINKIVEENSFANPDFINIGQDIFIPDAKPLDIFKGFLWPVHTKNIT
                     SGYGWRKDPFYGESQFHQGIDVRANYEWVRASKYGRVSYAGWLGGYGKVVVVTHPGGD
                     KTLYGHLSKITVREGQYVKQGQIIAKSGNTGNSTGPHLHFEIIRKGQHINPYAELKKK
                     RK"
     gene            complement(31882..32310)
                     /locus_tag="PLV81_04880"
     CDS             complement(31882..32310)
                     /locus_tag="PLV81_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOM09466.1"
                     /translation="MAGKSDSINSTIGEGSVFEGKFYISGSLRIDGKFEGEIKTDEEL
                     VVGETGKVKTNIHAKNVVVAGTVIGNIIADEEVKLLETAKVLGDIETPILTVQRGVVA
                     QGMVTITGGQKKDVKKLIEESYAGAPQAQTGIFGKKGQER"
     assembly_gap    32631..32730
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            32871..33527
                     /locus_tag="PLV81_04885"
     CDS             32871..33527
                     /locus_tag="PLV81_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOM09467.1"
                     /translation="MNTNTITLCVNEIFYSLQGETTRTGFPSLFIRLAGCNLNCRWCD
                     TPYARTGLPVTIAHIIQTIEQYPTLNHITITGGEPLMQEHTLTLLDILCRKNYPLQLE
                     TNGSISVEYVPPKVHKIVDVKTPSSGHADSFNISNIQYLKPHDEIKFVIADDYDYNYS
                     KEFMKNYLANVPCVINFSPVWGTMEPQQLAEYILHDRLNVRLNIQLHKILWHSEPKYF
                     "
     gene            33536..34408
                     /locus_tag="PLV81_04890"
     CDS             33536..34408
                     /locus_tag="PLV81_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOM09468.1"
                     /translation="MDYVGHIIRPPSEAYSMIIQVTVGCSHNMCTFCGTYKGRKFYIK
                     DLNQIKRDIDEASRYHFKRVFLTDGDVLILPTQTLLEIISYIKSKNPHIERIGVYGNT
                     KAILKKSLSELQELNAAGLGIVYQGIESGNETVLRKIRKGALPHKQLEASEKIKQAGI
                     LLSQTVLLGIGGLELSIPHAIDTGKHLGAMSPDYASALTVMLLPNTPLYRDYVQGKFA
                     LPDKFGLLRELKLIVEHMDVKNTCYFTSNHASNYLPIKATLPQQKNDVIQLLDEIIQR
                     QDESLLKPEFLRAL"
     gene            34421..34582
                     /locus_tag="PLV81_04895"
     CDS             34421..34582
                     /locus_tag="PLV81_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09469.1"
                     /translation="MDKSMEDAIRFISFELQDNPGADIAKLIEKASQQFDLTPLQTEF
                     LVNKFILNK"
     gene            complement(34617..>35111)
                     /locus_tag="PLV81_04900"
     CDS             complement(34617..>35111)
                     /locus_tag="PLV81_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09470.1"
                     /translation="VMKKEGEATPQAIEKVVAAAKEEVVIKKEEVQKFKPEDFKEEKQ
                     EIIKIEEKPKEVVKEIAKVVKKTRQIPQPEGQLLVTRYEIYFVKNGRVEWEGKVINPP
                     TKTEDKIYIASGDYIFCAKLDGTVLWRKKLANDGKVELEGEKVAVYAGGQKKLLDKLT
                     GEEE"
     assembly_gap    35112..35211
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            35558..36763
                     /locus_tag="PLV81_04905"
     CDS             35558..36763
                     /locus_tag="PLV81_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09471.1"
                     /translation="MDRSKLIQILHGIIIADALASPLDTLSAEHIHNVFGTIHDYTDP
                     APALKNKLYLWKKPALYTALSQYCILLSALNAQQDTYVHQHTCNFISSLGDSSRIFRH
                     PQGFLQHLQSPYDAPTAELLICIPALFFLHKQNPISIIHFILSHNKNAATCAASVFVY
                     MLLETIIMRQLIALDSVVLKQTAEDAYQFVQEHSALFFNNGGNPQNIIEATKDLHDLI
                     INLPLESDETHYTRYCIPFANRWSKNEYTRLTVNHPFTLLPLALYCVHTGQKDSLLYS
                     AIHKGGKISLLTPLVALLATAMYGYEVIPQNLMDTIVNKKRILQLLALLQSHSISINY
                     LTDFFQNEAKLTQKELEERESKLKHIKSKQTGKNKDKDTYTSMTYHVVESWTKLDKAK
                     WKKERRRKK"
     gene            complement(36823..37716)
                     /locus_tag="PLV81_04910"
     CDS             complement(36823..37716)
                     /locus_tag="PLV81_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09472.1"
                     /translation="MRHNNCYYCGQNGFIFRSYRFEDLAIVADIKIEKKHEGWPGIPH
                     GGLAMTTFIELIDIAKEFPAVFPYEYRFRFAGEQLKCGDMVRCIVQKGDARYFFGSFG
                     KSLQQPYCNCRYSEGHVHEYGNEIITILKNNMDASTPFIIPNMSTKLTNTVISDHDQR
                     IFYITDYTTDRTYLILHLHGKNNFAQAPDGSIHNGVLSSLLDETMGWSTFLSVWQAGV
                     TVELQITFYERVYNTDKFHIIGFCDDIRGEYGKKITTAYGGVVAQRGEELAIIALAYG
                     RWLTLPGFKELMVQYLKHYIE"
     gene            38002..38880
                     /locus_tag="PLV81_04915"
     CDS             38002..38880
                     /locus_tag="PLV81_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09473.1"
                     /translation="MFFIKKRNFLVLVFILICTTAAIAIDFNFKPIIIEEISRYNEDR
                     IAYQHVQKQIAPNMDNSFSALLIVKDRKIYLIQDGYDNPELINTKRLQMEMETKLIGD
                     LWENKINNKPDYVRITDRKVELLKNFSEDFVSKNFGTFFLNVRNAFIKKHVEVFKKLM
                     VDRKESGLIVTYTPLPVPAYLNAPETPTKYKITVSGKTIDEKLYYAEDSDGDGITETF
                     MVNSADGFNWGYKSGANIIFIYNNLDEEIKGLIGQLCNWAYYGTPEEEKEILQNFPKD
                     SDIINEFKLEVPQTTK"
     gene            complement(38931..39815)
                     /locus_tag="PLV81_04920"
     CDS             complement(38931..39815)
                     /locus_tag="PLV81_04920"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOM09474.1"
                     /translation="MAHRIDLHIHSHYSEDGDYSVDYLFELAAKKGLSAISITDHDSI
                     ESIPDALNASNIYGVEYIPGVELTTIYDDGSQQHILGYYIHENPQLNATLKQIGQFRW
                     EIAKKRIKKLQEIGFSMNEDNVWQKANNRPPTATAIMLEVFENPENRNDKRLQEYFEG
                     KKSDNRLMFFYREYLSENMPAYVPFISIPTKEGIDVIKSSGGIPVLAHPKFVNGEEHL
                     RNIVSMGIMGIEAISSYHNEHDIAFFMEFAVQHNLVITAGSDFHGPTSKPKVSLGGIK
                     DNDYSLLEELKKLHKKYS"
     gene            complement(39833..40555)
                     /locus_tag="PLV81_04925"
     CDS             complement(39833..40555)
                     /locus_tag="PLV81_04925"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOM09475.1"
                     /translation="MGIKKIVLVFFVIVTTVTFANELYFIDNEHIQRYNGKSGKWIEV
                     STLKQLKAYAEQFGTSIDEVYVINSINEKKFKKSYVFVPYSDAYIQMLMEKGISLQPV
                     NISYNEFIWPIGDPEKITSGLGRRWGRFHPGVDIPAPKGTLVRAAMEGKVIYAGYCGN
                     YGNSITLEHRDNYITRYAHNSVLLVKVGDYVQKGQVIALVGSTGRSTGNHLHFEIRCA
                     DIPLNPLDILPEKNLIIMKNPK"
     gene            complement(40704..41528)
                     /locus_tag="PLV81_04930"
     CDS             complement(40704..41528)
                     /locus_tag="PLV81_04930"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOM09476.1"
                     /translation="MIKIDNLLIGISFVSVLVVSLYIGVYYKTDTYIENEKEVYRAIA
                     HKNITRLHKVPILLYHNINGKGPYSLPFDKLKNQFTQLQNNGIKVISLQQLESHIDEH
                     NPFGEKVISITFDDGYYSMYTMLMPMARSMSYPVTLFVYIDAIAMGGTKNLTWKKLQI
                     MQNNNIDIQCHSHSHADLVKLYNQGTLESRYALYREIYLSKKILELYLDKTIDYFAFP
                     YGRYSIELTELCKDAGYKRVFSTDYGPNIVTRDNFCLRRHHVKSNYSDEKVLSLAW"
     gene            complement(41525..>41717)
                     /locus_tag="PLV81_04935"
     CDS             complement(41525..>41717)
                     /locus_tag="PLV81_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09477.1"
                     /translation="KAKRIKKITGVLSADTIAQSIMKAILKRKFMIVPGFMGKTTWLL
                     HRLLNGYGTRFLTDILIKL"
BASE COUNT        12883 a         8303 c         7343 g        12788 t
ORIGIN      
        1 ttattatttt gcttttgtca agtatttttt atttttccat tattttgtat gtgcacagga
       61 atagatattg tatgtcactt tttttattga ctttctttat gtattatatg tatctttatt
      121 atgtaaaggt tattgaaatg attcaaccag tcaaacataa cccatctata cctgtcatta
      181 tgcagcataa agcaagggtt ggtattctgg aaactcaaaa tgatgtcctt gcccccatga
      241 aacgttttaa aaaagaactc cctgtaccac agttagggca gcaacccaat ctttcacttg
      301 aaaaagtaag aatacgccaa aaagaatttt actcacaccg tggtcaggta tatgaaaaat
      361 tgcagaatac cagcgctgct attgcttcgt atcaaaaagc atcatattat gagccatcgg
      421 attcaaatct ttatggtcag atcaaacgct taaaaataca gagcctgacc aacctcaaag
      481 cctgatatat cattctccat cgtaaaaatt cttgatatta taatccataa taccgatttt
      541 ttaatataca gtgattgctt taaaacttat ttttttgttt acttttattg tgagtatact
      601 atccttgaag caaagatagt aatttgatat ggtaacggca acctatggtg caggaacagt
      661 ttaaagttgt aaattattta tctaactctt tcatagttgt tgaaggaaag cgcaacgccg
      721 ataattttta tattatccgt cagggtaaag tcaagcttgt caaggaaaac cctattgctc
      781 aggaaaccaa tcctttatta ggaccagggg atttctttgg ggttatcccc tgcatgagtg
      841 ggcatgccca tattgaaagt gccgttgctt tgacggatgt atcacttatt tcagtacagc
      901 gcgaccagtt tggcatactc atccagaaaa accctgcggt tgccatgaag attatacgct
      961 tcttttcacg gaaactccga gagtttgacc aggctattac acgactgacg tttgccaatg
     1021 ctgttgaaga ggatcccgaa cacctcttta agataggcga atattacctt aaaaagaaaa
     1081 acctgcccca tgcagcatat gcattacagc gctatataca gcattgtcca aacggtacaa
     1141 accgggataa ggcgatagct catttgaaat caatcaatgc cccgctaaag gttcctgaaa
     1201 atcctcagaa aaacagcctc acacggctgt ataaagataa ccagatgata ttctgtgaaa
     1261 acgagccggg agatgaactc tacatcattc agggcggcaa ggtaaagatt accaaaattg
     1321 ttgatgaaga ggtgttgctt gcagtgttaa agcctggcga tatttttggc gaaatggcat
     1381 tgctggaaaa caggccccga agcgcttcgg caataacatt tggcgatacc acactgatgg
     1441 caattaaccg gcaaaatttt gaaactatgg ttcagacaca accgcagctt gcaaccaggc
     1501 ttatacaact tttaagtgag cgtatctgga cagcctaccg gcagcttgag aatttgatga
     1561 tacgtgatcc gttgggcaga atgtatgata cattgcttat acaggtagaa aaacaaaaag
     1621 tccgtatagc ccccaaagaa agctttactt ttgattttgg ggttaaagag cttctcaaca
     1681 tggtgggaat tccacaggat aagggcgacc atttagtggt ggaattactt gaagacaaga
     1741 acataacact tgatgaaggc aagctcatat gtaccaattt agaagagctt gaaaagactg
     1801 taaactttta taagaaaaaa tcagctttag aacgcaaacg tgaagccagc aaaagtagtt
     1861 aaatacagcg ctataatact tatacttata atcatgctgc ttgctgcttt tgcagcagca
     1921 tttgtctatc ttttccctaa agaaacattg aaatcaatta ttgtacaaac actgggggat
     1981 tccttaaaca ggccagttac tataggagca atcgattata gcatcagagg tattcagatc
     2041 actgatctgt acatatacga cactgacaaa gaaaagccgt atattgcgca ctcaaaggat
     2101 gcagcaatca ggtttcagct tgtaccacta ttacagaagc aatttattat taactacatt
     2161 tcactcaatg atgcaataat tcatatactg tattatacag aaaataaaat aacaacgtca
     2221 aatcttgaaa aattgctcaa agaattatta tcaaaagaaa aatccaccat aaccacccag
     2281 attaaactaa tatcactcaa taatacaacc gttattcttg aaaacccacc ccaaaatctt
     2341 aaacccttag aaggaaaata cagtattgat actcatgtgg aactgcatga tgatattctg
     2401 gaattgaaaa acctttcaat aaaccttccg caaaacagag gaaaaatttc cggtgattgt
     2461 tccataacta ttaccagtaa ttataaaatt cagggcgatt tcaccctgcg tgattgtgat
     2521 ctactatggg tttacaactg gggaaagaat ctggcaacta tattgcctta cagagtattt
     2581 tcaggaacta tcgacaacct tttgataaca aagagtgctg ttcaggggac tgtaaccggc
     2641 agttgcaaac tcagtaataa tgcaatgctt tttcttagca atgcgttcac caatgttaat
     2701 ctcaataaaa agactgtcac tgtagcaaat gcaaaaggaa aaattgaaaa ttcaacgttt
     2761 gtactgaacc agttgctgtt tgcttttagt ggaaaaatcc agaatatcaa agtaagtgat
     2821 gcatcaatta atacaaaaga gatagctcca ttacttggaa aaattgatat aagcgatatt
     2881 gctggcgaac ttaaaggaaa ttttgaatat aaagacaata gtattgatgc tgatgtaacg
     2941 ctatcaaacc tttctattgg gaaaaaaggt actatactat cattacagga aacaaatctt
     3001 ctaataaaga ataatcaatt taaaaaagat ggcatacagg caaccgtata ttccgtaccc
     3061 tgtgtgttat ctgttgctac ggtagattca tcatttgaaa aatttacggt aaatattgaa
     3121 gctgatgaaa ttgacctcaa caaaatcatt tccaggcagc cccaaaaacc tgaacaacct
     3181 gaaaaaacag aaaaagaaat acatataccg tatgaactat caggaaaatg tattatacaa
     3241 aaattataca aagacaacct ggttattgaa aaaatcaact gcaactatac catgtcaaaa
     3301 aatatcattg cactacccac attttctgct aaagtatttg gtggagatat taccggtaga
     3361 agcgtcatca ccctttcatc ggaaaaccct cagatcacac tggttgcaaa atttagtaat
     3421 gcacgcctgc agaatataag ctcatacttt caggaatatg aaaacagact ctttggcaat
     3481 atatcaggac aattgcaggt aactatcgta cccaaagatc ctgttattga tagaataacc
     3541 ggcaaggttg aagtgtcagt tgaaaatggc aaacttgcaa acaccgggtt acagaatgga
     3601 ctgagtatat ggctcagtga tttaaaatac aaactgagta atctagagtt tcagaagatt
     3661 tatgggaatt ttaatctcag tggtcctgct attaccatca attcatttat ctttaatgca
     3721 cctgatatac ggctaaaact aaatggtcag tacaaccgcc atgaagaaag tgatgtagct
     3781 atttcactgg aattcagttc aaactttatc caggatctgc ctaaccctgc tctgctgcaa
     3841 cttgcaaaat acaaaaaagg ccggtggtat atcattccat tcagggcaca gggtaaagat
     3901 atctttgaag gcaaaaatat aaagcaggtg caataactta ttttttcagt tcggccttaa
     3961 ccttggccat caggccacct tttgcaatga tatcctgcat aaatggcgga aatggctttg
     4021 ccctgtattc tttatttttt gtaatatttt ttataacacc tgaatcaaaa tcaatttcaa
     4081 tctcatcacc ctgttcaata tcctttgctg cttcctctga ctcaatgatg ggaagaccaa
     4141 tgttcacgct atttcggtaa aatattcggg caaaggactg tgcaatgaca caggaaatct
     4201 ttgctgcttt tatagctatg ggagcatgtt cccgcgatga gccacatcca aaatttttac
     4261 cggcaacaat aatatcacct ggccttgcct ttttcatgaa atcagggtca gcatcttcca
     4321 tacagtgctg tgccagctca tcagggtcgg aagtattcag ataacgtgca gggataattt
     4381 catcagtatt gatatcattt ccaaatttcc aaactttacc atgtgctttc atgctgtacc
     4441 tcgtgaatag tttcaattag cgatagctca tgttatcgca actcatctgg atgagcaatc
     4501 cttcccagta ttgcagatgc tgctgctacc gcagggcttg ctaaatacac ttcgcttttt
     4561 ggatgcccca tacggccaac aaagtttcgg tttgtggtgg caacacaccg ttcaccttcg
     4621 gctaaaattc ccatatgtcc ccccaaacat ggcccgcatg tgggagcaga tactgccgct
     4681 tcagcttcaa taaatatatc tataagacct tcttccagag cttttctata tacttcctgt
     4741 gttgcaggaa tgatgagcaa tctgacatct ttatgcactt tgcggccttt taatatctta
     4801 gctgccaccc gcaggtcctc aatacgcccg ttggtacagg agccaataac aacctgatca
     4861 attggtatcc ctgcttcgcg agcaggttta gcattttcag gcagatgcgg aaacgctacc
     4921 gttggttcaa tttttgaagc atcatattct ttaatttcca catagcgtgc atcatcatcg
     4981 ctgtaataca gtgtataggg aaggcctgtg cggcttttaa tatattcaac agtcttacca
     5041 tcaggggcaa agataccatt ttttgcaccg gcctcaattg ccatattagc catagtcatc
     5101 ctaccttcca tggataattc agcaatagta tcgccggtaa attccattga cttatataat
     5161 gcaccgtcaa ccccaatgct gccaataata tgcaaaatga tatcctttgc tgatacatac
     5221 ggttgcagtt tgccatacac tacaaatttg attgattcgg gcaccttgaa ccatgttttg
     5281 cccgtggcca tcgctgcggc catatcagtg gaacctacac cggtcgaaaa tgcaccaaac
     5341 gcaccatagg tacaggtatg tgaatcggca ccaataatac acataccggg gcgcaccaaa
     5401 cccaattctg gaagcagagc atgttcaaca ccaaccctgc ccacatcata aaagtgaaca
     5461 atattatatt tctttgcaaa ctctctcagt attttaacct gctgtgcaga ttttatatcc
     5521 ttgttaggag taaaatgatc cgggataagc gctatttttt cattatcaaa aacttttgat
     5581 acacctaaat cttcaaattc tttaattgca ataggggcag taatatcatt acccagtact
     5641 aaatccactt tagcttcaat taattcacct ggttctacac gttccttccc ggcatgtgct
     5701 gctaaaatct tttcagaaat tgtcatgccc atactatttt tcctctacat agatatttac
     5761 aattacatgc aattgcaata gtaaaaatgt atcatccttt tatatatttt cagttttttt
     5821 atggcagttt tggcgatagt tactgttaat atactatgca gggattgtct ataaaaaatg
     5881 gtcgttgaga gaattccata aacattattt tatcataatc ttctcatctg gatataatgc
     5941 actcatctcg atacgccttg aaggctactc gatgaccaca tctcgatacg ccttgaaggc
     6001 tactcgatga ccacgtctcg atacacaacc ggtcactgag tgcgtgcgca gcacgtgtat
     6061 cgaagtgacc ggagtgctgt tgtctcgata cgcccttgtt tgccatgtat cgaagcaccg
     6121 gttgccgagt gcacgcgaag cgtgtgcatc gaggcaccga ttcccgaaac ttctcgctac
     6181 tctcgaatga tagggcaagg atttgccgtg cagggaagca cggcaaattt ggatgcgggc
     6241 gaggacctga aggatgctat tccgcagccc gcagtggaaa gtttaatatt acagagagta
     6301 aagggcattt ttctttggta ctttcttttg ttgcccggga caaaagaaag tacataaaag
     6361 cattatcatt ttaaaggaat aaagcgactg agaaatctta ccctatggtt tgttttgata
     6421 caacgccttc ttataaatta tacattctct acaagaactg caccacccat tccaccacca
     6481 acacacaggg tggcaatacc ccattttgca ttacgctttt tcatctcata gatgagcgat
     6541 acgatgatgc gagcaccggt acatccaact ggatgaccca gcgctatccc cgatccattg
     6601 acattaactt tgcttctgtc aaggccaagt cctttttcac aggccagata ctgtgctgcg
     6661 aatgcttcat tgagttctat aaggtctata tcatctaatt ttttccctgt ttttttgagt
     6721 aatttttgaa ccgcaggaac aggtccatat cccatcaggt gtggttcaac acctgcaaat
     6781 gcatttcctg caatccttgc cagtggtttt aatccaagtt cactggcacg cttagcggta
     6841 gtaagcacca gtgcagcagc accatcattt atccctgaag cattaccagc agtaaccgtt
     6901 ccattctttt taaatgcagg cttaagggct gacaagcttt ccagcgtaac atcacggcga
     6961 acatgttcgt ctttttcaaa catttttggt tcgcctttct tctggggtat gggaacagga
     7021 actatctcat cattaaacct cccggaatca atggctttag cagctttttt atggctttca
     7081 taggccacct gatcctgttc ttcccgtgaa atagaatatt tttctgctaa attttctgca
     7141 gtttgcccca taatatatgg ctcacctaac aggccacttc ccgaatgaag cagttcccac
     7201 atggcatcag taagctcaga gtgagtaagc cgcaaacccc atcgtgcttt ccacaacaca
     7261 tacggtgcat ttgacattga ctcaacacca ccagcaagta ctatatcaga atcacctacc
     7321 ataagctgct gcgagccaaa gaccactgca gtcattcctg atgcacattg ccgctgtact
     7381 gaggttgctg gcacttctac aggaataccc gctttgattg ctgcagtacg tgcaacattt
     7441 gcctcatcag atcccataag acagttacca aaaattacat ccgacaaagc acttccttca
     7501 acatttgccc gccgcaaagc ttctttcatt accagtgcag ctaattcata cgctttaaca
     7561 tcttttaatg attgcccaaa actgccaata ggcgtcctgc acgcggaaac aatgacaaca
     7621 tcattatcag ccattaccta tcctccatgt atatagtatt aaaataaaag atgcccaaca
     7681 accactacag gatttaagtg gttatatcaa acgcagtata aatatatatc cccatcccca
     7741 aatggggcag gggtatacag tattgttact gtattacaaa tatatttcta aaatggtagg
     7801 gttattttgc aagcctttaa ttatctggta atcatttttt ggatataatt atcactttac
     7861 cggcagaaat ttattttcaa actcttcttt tgaattaatt gaaaaaaact tatccaggcg
     7921 tgaagtcttg aatatagtaa taactgatgg agcaacatta aaaagtgcta aactaatctt
     7981 tctatttgtt gcactgttca taaattttac cagggtgccc aatgccgatg aatcaataaa
     8041 cttcagttct ttacagttga acccaataac tcttggtttt ttttctaacg catcattcca
     8101 cgccttttca acatcacgaa tgacatcaat atcaaactca ccttcaaggt ctatgactac
     8161 tacatctgct aactggttta ccgaaacatt aagcatggcc aatcacctct tgtaagcttt
     8221 atatgctttt tagtaacgtg tgaattgtac attataaaaa tctccatcgc aagcatattt
     8281 ttaaatgcat cagaaaaaaa taatttacaa atactacaag ctatcgtaca aataccgcag
     8341 tttatgtcat gtttatattt tatatataaa gggttatact atgaggttcg cactactgtt
     8401 aaaagtactg gcattaatat tacgcattgc ttcaaagcgc aatgaagact tcaaacgctt
     8461 tattggcact gtcagtgtca aaatcgcaat taaaacaaaa aataataaag gacgaacatt
     8521 catttttaac aatggttctg tctcttcaac acgttcgctc aaacagtatg atgctgctct
     8581 tgtcttttcc gatgctaaaa ccggttttac cgtattaaaa gaaggcactc aggaagcatc
     8641 gttttacgcg gcagcaacag gaaccctgta tgttgaaggg atggcttttt tcattcaatg
     8701 gttcaatgac gcagtaacta tcgccatgaa aaaaaacaaa aaataaacag gaggaattat
     8761 gtcaataaac ggtggacact taattggcaa atatttagct aaacaaggta tacagtttgc
     8821 ctatggcata tcgggcgggc atattgaggc attgctggat ggactacagc aatatggcat
     8881 acgatcaatt gatgtgcggc atgaacaggc tgctgtgatg atggctcatg ccactgctat
     8941 ctatactggt acacctggtg tgtgcttttt aaccgcaggt cccggcttta ccaatggcat
     9001 caccggtatt gccaatgcat gtttagataa tgcaccggtt gtggtgttat gcggtcgtca
     9061 tccgcttcgc gatgatcata aaggtgcatt gcaggaaatg aaccaggttg atgtggtaaa
     9121 gccattggtg aaatggtgtg ccacctgcta tgatactaag cgcatcccgg aatatctgga
     9181 tatggcattc agacatgcca cgggtggaag gccagggcca gtatttttag aactgccccc
     9241 ggatgtactt gggaaagctg ttgatgagtt tgattttaca cccagcgttg atgaatttaa
     9301 agctgtgccc gattcatcca gcttatataa agctgctgaa attataaaca gcgccaaaaa
     9361 gccacttttt ataggcgggt caggtattgg ttacagcaac tgtactctgg aactgcaggc
     9421 ttttattgaa aaaacaggta ttccgtttat actgctgaac aatggccgcg gcgttttgcc
     9481 cgacaaccat ccgctgtcaa tctggaatac tggcaatgtt ggcctcatga tgacaatgtc
     9541 acaggctgat gtcattgttg cagcaggcat aaggtttaac tggctttttc aatcaggtgc
     9601 tgtcattccg ccaaccacaa aagtggtgag gattgacatt gaacccactg aaatcaatcg
     9661 caaccgtgtt gctgatgtgg gccttctggg tgacactggt gcagcactga aagcattgtt
     9721 gccactggtt gagcaacgca atcatcaaca gtggcttgat atgctgacac aggcagggta
     9781 tgcgtttatt gacagtgaaa ttaagctgcg ccagaatccc agtgacccca tccaccctat
     9841 acggctggtt gcaaaggctc aggaagtgtt tggcaccgat gcgctgtacg ttgttgatgg
     9901 cggtgatact acttactttg gtctggtggg ttttcagtcc aggcacaaag ccggtgtact
     9961 atcgcaatcg gctgggcttc tgggttgttt gggagcaggc atcccatttg ccattgcagc
    10021 caagctcatt catccggata agcaggtagt ggtgctttcc ggcgacggtt cgtttggatt
    10081 taacggtttt gaatttgata ccgctgtgcg ccacaatata ccatttgtat gcattgtaaa
    10141 caatgactgt gcctggggaa tgatcaaaca cagccaggaa ctttctattg gcaaagaacg
    10201 ggtaacctgt gctgagcttg gcatccgaag atatgaaaag gttgttgaag gcatgggtgg
    10261 acacggtgaa tttgttgaaa aggatgaggc aatagcacct gcgcttatcc gtgcaaagga
    10321 aagcggtaaa ccggcatgtg ttaatgttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnnat gaaacgtata tttgaaatac acgaattaag cgcaacccta acaccagtat
    10501 ttacaaaaca taatattata tttgcatatt tatttggttc aatagtgaat aatgagtcta
    10561 cgcccacaag cgatattgat attgcagtat attgcaaagg tgttatggat gacagtgtat
    10621 ttgaaaaaaa gctatcatta catttagata tatgccgaac actaaagatt gataatgttg
    10681 atatcatagt attaaataaa acaaccaatc ttatgcttat tgatgaagtt atccgtaatg
    10741 gtatattgct ctatgatacc gatacatcat tgcgtgaaaa atacgaatat tttatgattc
    10801 ataaagcaat cgattttaaa actcaaaggc tatatactat gggtatctaa tggaaaggat
    10861 catacagaaa attgggaaaa tacatgaata cttaacaatc atttatacta ttaaagatga
    10921 ttgtataaac aaaatatata ccgatcctat ttataaaggt gcattgcttc attatctgta
    10981 tttgttatcc gactcatgta taagccttgc agaactggta ataaagtaca aaaaattacg
    11041 agttcctcaa acataccatg aggctattga tatacttggg gaaaataaca tactcaatcc
    11101 cgactttgca tattcttttg ctaaaatagc aggttttaga aattatcttg ctcacgatta
    11161 tgaccagctt gatacagcca ttatatgtaa tgaaatatta aagagaacga aagatattga
    11221 agaatatttg gtgcaaatta agaattcatt aggaataaag tattacaaat gatatatata
    11281 aatactatat taatcattac agtactgtta tgaaagcact acagtttaat gtaaccatac
    11341 cacaatgggt agcgctcaaa gcgctgggag catgtacaaa atctgtattt tacaaaagcc
    11401 ccattggcac tctcaaactg gttgaaatac cagagccaga acctattaac aacaactggg
    11461 caattgtaaa aacaacgtat tgtggctttt gcggaagcga cttcaatctc attttgcttc
    11521 atgactcgcc catggcttct ccgtttacct catttccctg tgtgattggg cacgaggttt
    11581 gcggcaccat tgaagagccg ggcaataccg gttttaaaaa aggacagcgt gttgttataa
    11641 atcccatgct ttcatgtgct gtgcgacaat taccgctatg cgcttcctgt aaaaaagggc
    11701 gccctgctaa ctgtgaaaat tttgccaatg gtactatttc accaggaatg tttaccggta
    11761 tttgcaagga tgtcaatggt ggctttgcag aatacctgcc ggcacaccgt gcacagcttt
    11821 ttgcaattcc tggtagcata agcgataagg cagcagtgct cactgagcca ttaagtgtag
    11881 cattacaggc aatctatgat aacatgccac aacattccga tacggtgtgc attattggct
    11941 gtggtgttat tgggcttatg ctcgttcgtg cattgcgtgc gttaaactgc aaagcaacaa
    12001 tagcagtaat tgaaccatct gaatttcaca ggcaaaaagc tctaagcttg ggtgccaatg
    12061 ctatcgtaca gggtgatttg ttagagcatg cagctacatt taccagtggg aaagtatata
    12121 cccccatgtt tgggccaaag atactgcagg gtggatttga cagggtattt gatacagttg
    12181 gttcatcaaa aacattttca cttgcaatca acattgcacg ggctggtggt gtagtaagcc
    12241 ttgttggcat aacaagcaag ctttcatacg atccaactcc cctatggctt aagctgttaa
    12301 ccgtcaaagg tgtttatggc tatggcttta taagggaagg cagcaaaaca aaacatatct
    12361 ttgctaaagc attggaattg atgaagaaga tgcccgatat tgaaacaatg gttacccaca
    12421 cattcagtat tgaccagtat aaggaaatga ttgaggttaa tagcaacaaa aatgcccata
    12481 aagcaataaa aacagcaatt acattttaag aaagcaacac acagtagcaa tcaatcataa
    12541 cttcatatgg atggaattga tgttttacaa cccattcatt tttagctttt accaaaacag
    12601 aatcaattga ttgaactatt tctgcattgt agaatggctc ttcatctttg agcgaacgta
    12661 taatgtgcat aatttcatcc atgtgttcac tatcatagag tatcttttct ttgccatcaa
    12721 aaagaacagg ataatatcta tttttacatt taaaaagaat gtatatcaca ataggttctt
    12781 tcaggtttga tttttttata ctgcgtattc ctgaaggcag gcgtaaaact tcacgtttaa
    12841 cagactcatc aagctgtgct atatcatcaa taaagcctga cgtatggagg ttgtcagcag
    12901 tttcaataaa ttccaatact tcgtctaaat tgagatcgtg atcaccaaac aatgctattg
    12961 attttgccag attgatcatt tcaaaatctt ttccgccatc ggcaacaagt acaggtatat
    13021 ctgcaaatga gcttatctct ttattgcgcg ttaacaggcg gtttttccat cgcgatgcgt
    13081 gatacacaat gtcactgccc attgttgatg gaataaaatt aaacacatat acatcacgtg
    13141 gttcaaacca tggacgcaaa attcgcccca tgcgttgggc aagcgtgagt accgtccatg
    13201 gcaaatcaaa gttaaccaga atccgcgcat cctgaaaatt gaagccttcg gacattgcac
    13261 ctgttgccac aagcacctgt atttcttttc cattggcatt gttttcgtca tctacataat
    13321 ccgctttgtt tgctattggt gcaaaccgct gtataatgga ttcaatttta ttggtatcag
    13381 catcaaccgt agattcaaca acaacgtgag gcagtattcg ttgcaatgca tcttttatgt
    13441 agcgtgctgt ttctcgataa tggcaaaata ccacaacctt ttcatggtta aaccgctcaa
    13501 tcaggttagc taatttttgt atcttttcat cacgttcata cagcaatggt tgtaggcgca
    13561 ctctactatc aacagcaaat gagctcaatc tttcttgatt atgaaatcgt attttatcaa
    13621 agccatcttc acgcgaaagc ttgtcaagca actcatctaa ttgttttaac gatgaacacg
    13681 cctggtgaac aatctgagct tcccacaatg gatcgcgtac acctgcagta tcatgaaaga
    13741 gcgtgttact ggtatcattt acttttctat acagaagctt gctttgtaat aactgatgga
    13801 ggatactatc gccctgattg gtatattcaa tattctggaa atgcagttta tggggaaaat
    13861 agcgcttttc ttcatgtgag aagagcacat aacgatttcc tgcctcatct atagagctat
    13921 agtatttaac aactgttggt gcagttaata caacaacggg cagtagctct gatagctccg
    13981 atgggctttc tatgcaccat ggttgaacac atttaccaaa caaaccatct acttctttgt
    14041 gtggcaacag aactaactgt gaattgatat catcaacacc cgtgctgtat ggtgttgctg
    14101 tcattaaaag tatttttgct ttgcgctcat gcacagcatc catgatacgc tgatgtgaaa
    14161 ggcgaatgtt atctgctgaa ccaatgtttc taagatgatg gctttcatca aggattatca
    14221 gcgtattttc atcaatgtgt gctaactcac ttaacaggtg tgccacatca attgattttt
    14281 tgaaatcatc aagcgataga atataatatg aaaactcctc gcttgacact cgtgcagccc
    14341 gcagtatcct gtgccacata cgcttgagcc ccgctggaca tagtaccaat gcagcattta
    14401 tacgtttact catgcaaaga tatgctgcaa tatgcgccgc cataaccgtt ttgccaaggc
    14461 ctgttgaagc aataagccag gcaccgctgt gctcttcaat tattcttagt agccgcgata
    14521 caattggttt ttgatatccc gcaagtggtg gtaatttgtc aggcaactca tcctcaggca
    14581 tatcatacaa aagtagcaat gagcgcatgt aaatatagta tggcggatag agagcaagca
    14641 attcctgtaa tttttctaaa agctcctggg ctaaaggttg agcttgacta aaatattcat
    14701 caaacttttc tacaaagaaa agcacatcat cctcattggt aacaacaatc cctgcttccc
    14761 tgctttgcac caaaccgctg taggtaaggt ttgcggatgt taccactgca ctatgcgtgt
    14821 cagcaatata gagcttggca tgttgataca aaaattttgg cgctagtgtt gtgaatgttg
    14881 ctggttgatt gcttacgttt accgtaaagt agccagcacc aagcgcctca cacaattcct
    14941 gaataaacgc tgttttatct tcaatgcttg ctgatgctaa atcttcaata aatttatcaa
    15001 tgatagtttc aatcttttcc tgcgtatctt cattgcgccc aatcagaagc cttatttctt
    15061 tatcttttaa cactcctcgt aataggttgt atcctgacgg ctcaaagtat gcggtagcaa
    15121 tgcgtacaat ggcagcatag cggattgctt cacgaataca gtgcaaagca acattactcc
    15181 caaaaaagcg tttgattata tctttatgtt ctttcattgc aaaagccaat attgaaatta
    15241 ttactttatt aactatctct attaaaatca aaacaatgag gcacaaaata cacaaaatac
    15301 atatacacag tatacaatac cttttgtata tcataccagt aaattacaat aaaaaaatgc
    15361 tttacagggg cgatagtacc ctgtataatt ataacgatat cgagtaaaat ggctatgtta
    15421 ttcatatctg agtacctatg caatgggtac aaaaaatctt tttgtaatca cgtaaattaa
    15481 atccaagtca tactgtttta agaatgactt taacaacacg tgtataatct aagggggaat
    15541 cttcttatga aaaaagttct tatcgttttt gttgctatca tcattgcctt aatagcaatt
    15601 gatgcctatc aatcgcacat tacctacacg tatacaccta aaaaattgcc tgcaacattc
    15661 gatgaatact accagatgaa gcttaaagaa agcaaagaaa aaggggctcg ccctcataat
    15721 gaagaaaagc ttttgaaatt tgcagataaa acaaaaatag caatgctcta tatccatgga
    15781 tatggtgctt cgcgtggtga gggtgaatat gttatagata ctatcgccaa aaaattaaaa
    15841 tataatacgt attacctgcg gctgcccggc cacggtacaa ctatggatga ccataaaagc
    15901 actgaatatt accagctctt agataccgca attgaagctg cacacatgac aaaactactg
    15961 ggtgaaaaac tggtaatcat tgggacaagc atgggtggaa ctattgcaac ctatattgca
    16021 gcagagcacc ctgatatacc cgatgcggtt atactggttt cgcctttcta ccgttttgca
    16081 aatcctgtag gtaatgcatt actctttcgt ccattcttta aaaccgtgct tctttttgca
    16141 aaatatcgcg aacgccatga tccatacgat gaccccaatg acaactggac tatgtactgg
    16201 tatgcaaaaa attactgggc atcactgcat tcattactgg acatgagcga tttaatagca
    16261 cgggatgatg tgtaccgtaa agtttcctgc cctgtactgc ttttgtatta ctacaaagat
    16321 aatgagcata aagacgggtc tgctcaggtt gaagctatgc ttgaagctta cgatatattc
    16381 aacaacggca agcccaaccc tttgagcaga aagatagcag ttgaagatgg tgatcatgtt
    16441 ctgctttcaa aatatgtgaa atcagattgg ggtaaagcag aaaaagctat tactgatttt
    16501 ttaaatgata tagcacaata aataagtttg atgattatta ttctataaat tttttgaaat
    16561 ataattccta attttatgcc atgataataa acagaaatta tatactatca tggcattttt
    16621 ataaagtggt gaataactcc catttttata gtagtctgaa ttacaactgt ttgcagtacc
    16681 cccaactatt gtattatttt acaatcttta ttacataaag gttacgagta tatttctact
    16741 atgttaaata tcaatatcta acttcttaca aaacaattta agtattgcca ctatcacaaa
    16801 atcattaaat aaaaaaaaaa cgcacatata tgtgcgtagt ataaatctaa taattttaaa
    16861 aggagttcat tatgagtaca attaaaagaa tattcattct ctttatgttt tgtgtaacaa
    16921 gcttcttttt tgttacaggt aaaagttatt ctgagggaaa aaacctttat ttccccattg
    16981 gcctgaccta taacagtacc tactggtgga ggggtgttga aatcaatggc aagggtgccg
    17041 gtgttctatg gccaaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatta
    17161 aaaaatcacc tgtgggctga tgtaggggta agcagcaggt tgtaaaaaaa tctcttgacg
    17221 gtgaatcaaa tttgaatagt gtgcctttta tcaatacagc aaaggtgtgt tatggataac
    17281 tatctcaaag tatcaacatt taaacgcttg atgtttaatg taaaaacccg cttatactgg
    17341 gaactatcgt ataagaaaaa gtttgcctcc attgcaggta accccattgt gtggggattg
    17401 tggaacattg atatttatgg acccaacata caccttggca aaaatgtggt ttttttagct
    17461 gccaatggca gtaaaaccgc cttaactacc gtacgacttg cgggtcatga agggtgtatt
    17521 gatataggaa ataacgtact tgtgatgaac ggcgtgcgaa taagcagtgc ctcgcatatc
    17581 gttattggcg atgactgcat gttagctaac ttttgctacc ttaccgatgc tgactggcac
    17641 gatatacatg accgcaccaa ccctgtgggc aagactgcac ctattatcct tgaaaaaggg
    17701 gtatggattg gcgattcagc cattatctgt aaaggagtgc gcattggcca gaactcagta
    17761 gttggtgcag gtgccgtagt aaccaaagat gtgccaccca atgtggttgt tgctggcaat
    17821 cctgcacgga ttgtaaaaga aattgatccc gataaagttg tgacacacag tactctttat
    17881 cataaggtgg gagcgcctcg tatatgagga tatgcctttt gtgttatcgt gccaaccctt
    17941 tttgcggtgg tcagggtata taccttaaat atgtagcaga agctcttgca cggcaggggc
    18001 atgaagtgca tgctatcgtt ggtcctccct atccccacca tatggatcat gttactatcc
    18061 actatattga aaataatcag tactatatta aaaaaggcgt tgaatgtatc acgtatgata
    18121 atccatttga tatttttcat cccattaatg cctatgaata tatccattca cggcttgggg
    18181 cctttcctga aataagcgga ttcagttacc gcgcattttt tatggtgcga aagctccatg
    18241 aaaaactcca ttttgatgtc atccacgata atcagtgtat tggctatgga ctgcttttca
    18301 tgaaagcact gggcattcct gtagtagcca ctattcatca tccattaacc attgacttag
    18361 aaaatgttct ggaacgtgct tcatcattca aaaacagaat gaaaggagtc atgttttatc
    18421 ccatccttat gcagcaaatt gtgtcaaaac gccttgacca tattatcact gtttcagagg
    18481 attccaaaca cagaataaca aaagattttg gtgttccgcc acacaaacag tcagtggtat
    18541 acaatggact tgatacttca attttcagga aattgccaaa tataaagaaa aaacacaata
    18601 agcttttatt tgtgggtaat gttgaagatg gcaagaaagg atttgtatat ttgctcaagg
    18661 ctttaaccct gatacagagt gatgccacac taaccgttat tgatggtggt gctccacacc
    18721 gtcgtatcac acagaaactt gttgatactc ttggcatgcg caacaaagta gaatttgttg
    18781 gtgccgcatc aactgatgaa ctggtacatc actataatga agcaaccatc gccattgttc
    18841 cttcagtgta tgaaggattt ggatttcctg cagccgaggc tatggcatgc ggcacgcctg
    18901 ttattgccag cgacggtggt gcactaaggg aggtggttgg tgatgcaggg attgttgtat
    18961 ctgcacggga tgaggtagca ctggccaatg ccatcgataa tctgttgcaa aataaaaagc
    19021 tattacagga actatcgcat aaaggaatta aacgtgtaca aacacatttt aactgggaca
    19081 atgctgcgtt acagatggca gctatttatg aaaaggttat ttccagttac tatcggtaat
    19141 ctgttatttt tgtattttta ggtagcttta gctcagcaac cgtatcaggc actgcttcat
    19201 ttatcacgta ctgctgcatg gttacctcaa atgttgtttt gccattaaca tcaacaaacc
    19261 gtaatgcttt acaggtaaac atgttattat ctatcttagg ttttaaaaaa tatatttcat
    19321 accggctgtt ggtgctcttt accacaaaca aaattttact ggggacattg tctttcatag
    19381 atatagtctg atgcattgtg tcattttcca gtaatataaa ttgttcgcta tcagcttcaa
    19441 gcgcctttac actataataa ttttttaaaa gcggtatctt gccaatcaac agattgtcta
    19501 ttagttcaac atcaatatta aatgggagaa aagaatatac agggaattta ttatagtcaa
    19561 acactaaaag attgtttgca attggttgat aaatataaag ggtatcatta tctcgcacca
    19621 ccacgctcag ctgagttcca aatactgaat caacaattga catttttgcc ttccttgcat
    19681 ccacattata gagcagtttt gctgatgact tgaaaccttc tttagatata ttttctattc
    19741 gcaacgatgc aatcactgta tgaggcaatg tagcatttaa ttttattatt tcttctaaga
    19801 gcagtgttgc acttgtttcc tttttttctg tttcttttac tggtacagaa gtacacccat
    19861 acaaaacaac cagagatagt attactacta tgtagcagtt atttttactt ttcacattca
    19921 tattactatt acttcccttt atgagtatca atcttttttt gcacttccgg ttttggatca
    19981 agtttatatg ctttgttcca gtactccagt gctttgtccg gcaattgcag ggcattatat
    20041 gcatcgccaa tatgttcata cacaactgcg tcaggatagc cttcaagctc taattcctgt
    20101 gctgcttcta acagtaaagg caatgcatca tgatatttcc ccattttgaa atatacccat
    20161 cccagtgaat ctaaatatgc tccatttgta ggttcacttt ccagcgccct gttaatgagc
    20221 attaacgatt catccaagcg tatgttgtta tcagcatata agtatcctaa atagttacaa
    20281 atccttgcac ttttctgttc attgttgcag gcttcctgta atacggcgat agtatcctgt
    20341 actcgtttct gtttatcaag agcagatgcc aggtaaaaat aataggtttc cgactttttt
    20401 aaatcaactg cctttctgag catatcttct gcatcaggat aattattttt ttgcatatac
    20461 accagacctt taaaaaaata cgcatcagga ttttctttat cctggttttg ggctgcattc
    20521 atatattcaa aagcctttgt ataatccttc aaaagtgcat acagatagcc aattcttaat
    20581 aaaacgtcat tttctttttt catggaatat gcaatggtat agtaatagat agctctgttc
    20641 agttgattgg tatcttcata taattgtcct aaatacagat atgcctcata tattgatggg
    20701 tcattagtca aagccagcgt gaacattatt gttgcagctt cataatcttt tttctgatac
    20761 agcattacac ccgcagtata aaagtttgct gcagcagtag cagtatcatg ttttctcatt
    20821 gcaatgcgag ccagtgcaat atacggcata tcaagagacg tgttcattct attaagcttt
    20881 tgtgccagct ctctggctgc atcgtcattg tgcagcagtt cattatacat catccgtgcc
    20941 agcaatcctt ccgagcttct gtgattaatg gctttccgca aaacaggcaa cgctttcata
    21001 tcttcataga tatagtatat tctgcctgca tgcaatattg ctttcagatc attgggatat
    21061 cgttcaagat attgtataat ataacttatt gcctgtttta tatcaccttt ctccagattc
    21121 agaagcccca gtatatacaa catggaataa ttttccggat tttcattata cgcttttaaa
    21181 aaatagtgat acgctttttt aaattcgcca aaactataat agatgtgccc taaataataa
    21241 tatgattgtt ctttatagta cgattcaatt gattcacatt cgctctgttc tataactttt
    21301 aaaaaatgat atcgcgctct gacattctgt tttaactgtg aataatagac ggttgctaca
    21361 ttaaaacgta cacggtacag ttcaggcctt ttctgcaaca gcttttcaag aaccgtaacg
    21421 ccttcatcag cctggttcag attcatgtat accgaatact gcaacacata cggcttatcc
    21481 cagttttcat ttactgcaat tgatttgctg gcatattcta ttgcctttgc ataatcatac
    21541 tgataataat aacattctgc aatctgatag taaatagcat ctaactgagt accttctttg
    21601 ctggcttctt caaagagttt gatagcttca gtatattttt tttcattttt taatagtaat
    21661 gccttgctgt acagttcaaa cgatgtttct ttatatactg gcctggcaat agtttcagtc
    21721 tttgtatctg gtaaagaact gcatgcaatg gcaataatca taagaagcgg caagaatttt
    21781 ttcatggcga tagttccttt taataacctt ttagtatttc ccggtacaca atggatagta
    21841 taattttccg catatggtgt cggggatatg cgataatacc tatgtatgtg tacaatacca
    21901 tgcacacatt aacgtatata acaatatata ctatttatgg taactgtcaa acataaaaca
    21961 tcgatataaa tcgtcatgga ataattttgt aacaaaaaca aaaggattga tacaaaaata
    22021 ctttgcaaat gcctcaatat tttgtatatt atacactgca tgcatttaaa ggcaacatac
    22081 agttcaatga aagtaaggaa tatgttagaa cattaaaaac ttttcaggag gtaacacatg
    22141 cttttgggaa ttgacccctt atactggatg atgatggcac ctgttcttct aatttcactg
    22201 tgggcttcgt tcagggtaaa aagtgcattt aacaaatatt ctcaaattcc atcacgatcc
    22261 ggacatacag gagcagatgt ggcacgtatg attttacagc aaaatggatt atcacatatc
    22321 ccggttgaag aaaccacagg ttttttatca gaccattatg accccataaa gaaagtagtc
    22381 aggctttcac ctgaagtata taatagcaat tcacttgctg caattggtgt ggcagcacat
    22441 gaaactggtc acgctattca gcatgcaaaa ctgtacaggc ctcttgttct gcgtaatgcc
    22501 atagcaccaa ccgcgtccat tggttcaaac ctttcatgga taattatttt tgcaggcttt
    22561 gctatgggtt cattagggct tgttaaactg ggaattttgc tttttagcct ggtagtattc
    22621 tttcagctta ttacactacc ggtagaattt aatgcttcat cacgtgcaaa ggaaatttta
    22681 caaggttatg gcattgtgag ttcaggggag cttgcagggg ttagcaaagt actatccgct
    22741 gctgcaatga cgtatgtggc tgctgcagca tcagcaataa tgacactgtt gtatttcctc
    22801 ataagggctg gactcttagg tggtagagac gaataaaaaa aggttataag aaataagatg
    22861 gagtgagcat attaaaaacc tgggtcagag taaggatatg taattgataa aaaaataaaa
    22921 ttgcaggctc tcaagcgctt gagtgcctgc ggtgttgtaa caaaatacag agttagaaat
    22981 atttaatgat ttgaacggcg agatccttcg tcgtcccaaa aagaaaggga ctcctcagga
    23041 tgacatagta gatagtacgt tgtgatctgt aataacgttg atttcacatc acatagaaaa
    23101 agcggacatt ttacgtgaga aacggcaaaa tccttcatcg tttcaaaaaa gctgtgactc
    23161 atcaggataa cggtatgagt gacaagtcag gacttgcaat gacgtttggt tgcacgtcac
    23221 tgcaagctaa gcgtggcagt cggtcacctt gatacttgtg ctttccaccc actcggtgga
    23281 cgaacactca ctacacacat ttcacgtcca ctcagtgatt ggcttcgtat gcccggtcct
    23341 ctggtgtttg tactagcatt tatatccaaa caatatgatc cgctaacctc aactcttgat
    23401 accatggttt taaataatcc ccataaatat ataaaacaaa agttacaaca ctaattaaat
    23461 tgttgacata tttattttgt tcttatatac aaaattaaaa tcaaaacgat ttatttcagg
    23521 aatatgatga aagttcccgg ttcagtacag gtactatcaa ttctcatagt atgcttgtgg
    23581 attactggtt gtgacacagg tgatcctttt attgacattc actctgactg gaaactgtat
    23641 tcaggagaca actactccat tgcacaacct gaattcgatg attcttcact gcaatcaatt
    23701 aaccttcccg ggaaactatc gccaaaaaaa tccaggcagt atctatggct aagaaaatca
    23761 gttgttattc cagattcatt taaaaattat gatattgctt ttgttttagg aaaagtgtgg
    23821 gatgttgaac agacatattt taatggttat aaaattggat ctgctgggag tgaatatcct
    23881 aactttcatt cagaatggaa cagcttcagg tactatgtaa ttcctttgca tattgttaaa
    23941 tacaaccagc caaatattat tgccatacga gtattcagta atcagaatgc cgaatacaat
    24001 ggcgtgccct taattactac acttgctaat gccaaagtaa taaattttta tcgttcatta
    24061 cttgcagaat atataccact tgcaaccagc ttccttacat tcctgttagc tattattgcc
    24121 ctgtactttt attttaaaaa caaagatgtg cttacattat attttgcaat tgtttccctc
    24181 ctgtggtgta taagtgcaat gcatttttat ttgccacatt actggattat ggactttaat
    24241 acacaggata aaatatacta tgcgctgact gcagttattg cgatagttgt atacttcttc
    24301 tttgaaaatg tgacagatag cttgaacagg acactcagga ttattattac aattgccgct
    24361 gcatgtatga taattttaag cctttctgct actgaacatg atcccgtaac aggctggcga
    24421 ttccaggtta ttggatcatt tggacttatt gttcaaatac tgtggggagc acttattata
    24481 aagggtatac gaaataataa aaaagaagca caaactctat taatccctta tatttttatg
    24541 atgatctgtg tgggccatga ttcacttgca gtatcaggga ttttttatac caactttttc
    24601 tggataaatt tggggtaccc cgcattaatt ataggcattg gcgcaatatt gtcacaacgc
    24661 tctgcaaaac tggcacagga actgcttgaa tcaaaacaat atattgaaaa gaagaatgct
    24721 gacctgatac agatagtaca aaaaatcaat cagtcaattg gggaattaca gcatgtagca
    24781 aaggaagttg aaagttctgc acgtactttg caaaatagca tgcatgatca gaccaccagc
    24841 attgaacaaa catcagcagc cctggaagaa ttttcaacca cggtagacat cattgcttct
    24901 aattccaagc atcaggatac aattcttaat aaaaatagag acctgttaat ggaattaatt
    24961 aaaggtattg gccatattac cgatgcagct aaaacagcag tcaaactgag ctataaaagt
    25021 caggggcaaa cagctgtaac aaaagaacac ctttctgaag cacttaaagg catagaaaaa
    25081 ataaaaaact attcggacac catattaaca ataaccgaaa ccattaatga tatagctgaa
    25141 aaaaccaatc tgttatcact taacgcttca attgaagctg cacgggcagg cgagtatggg
    25201 cgcggttttg ccgttgtagc tgatgaaatt gggaaattag cagatagatc cttagaacaa
    25261 tcaaaaaata tccaggcaat cttatcggac atagttactg atattgaaaa ccagacaatc
    25321 cttatgggca cagttgtata ctcaaatgaa gatgttgaac gctctgttta tatggttaat
    25381 catgcaatag acacaatcct tgatttttgc atatcacagg agcaattaac aaaaagcatc
    25441 gaagaaaata tggcacttgt attgcagggt tcttcgcaga ttacaagcgc cacacaggaa
    25501 gaaaaaataa ctattggaga gattagttca acaatacagc agcttgttac cattacaaat
    25561 gccgtatatg aaaataccga aatgttagca aaaaccctca caaaaatcct gtcacagatt
    25621 gatatgcttc aatccgtagt ccaaacaaaa aacaattctt aagaaaggat tatattacta
    25681 tgaactatcg ccattataaa aaaacaacac tattattttt tatactgtta gttgcaattt
    25741 ttctttcact gattattttt tttaaagcat tttatccttg tcccttatcc accataccat
    25801 caaaaactac agaacatgac tttttacagt actttgacca tcgtataaaa gttttaatgg
    25861 gaaaatatga aattcctgga gtaaatattg ctattatcag gcaagggaaa atattgtggg
    25921 cgaatgcata cggctttgca gacaaagaaa aaacaatacc catgaagctg gaaacaactt
    25981 gccgcgttga gtcaatttca aaatcagtta cagcgtgggg tattttaaaa ctggttgagc
    26041 gtggagctat atctctggat gaacatatta tcaaacattt gaaaacatgg aaatttccac
    26101 catctgcatt tcatcctgaa gcaataacca tacggcagtt attaagccac agtgcagggc
    26161 tacccctggg tacaatcggt tttatgtatt tttttatacc tgaataaaat aaaaacacct
    26221 aaaaatattt tcttaatata ttgacaaaag tgctactttt ttaaagtatt ctataatatt
    26281 cttaatattc ttactctaca ggggataggg tcccccgtta aacgccttcc ggctgatggc
    26341 tcctactgca tggcagtagg ttttttattt tacctgcgcc aaatttttta actatactct
    26401 atggaggatt ttatgctgac tatgtttgta gtagcgtcgg tgtggcttgg tttagcagtt
    26461 gtttccactc ttattgccaa cacatacttt ttgcctgaac atctacttga agccccaatg
    26521 gcagatgagg aaatacctca attagatgga ctaaacggcg caaataatac ataattatac
    26581 actataccat actgcataat gtggtatggt atagttataa tgtaaaaaaa cttttgactt
    26641 ttatatatgt acattatata ctgtgagatg taattcgata ttgatagcaa cgaaacatat
    26701 actatactat acagggtcag gggattgtta tgtgtgatac cattgttgcc accagtaaag
    26761 ccactgctga cggcagtgta atcttagcca aaaacagtga cagggaagca aatgaagtac
    26821 agttattaaa atatattgcc aggcgaaact ggggcaatga aacagccgtt caatgcacct
    26881 atatatccat accgcaggta aaaagcacac atgagatatt aatttcaacc ccattctgga
    26941 tgtggggttg tgaaatgggg gcaaatgaat atggccttgc tattgggaat gaggctgtat
    27001 ttaccaaaga accctatgaa aaaaccggac ttttaggtat ggatattatg cgtctggctt
    27061 tggaacgctg taaaactgca tatgatgcac tcatgctttc caccgaactc atagaacgct
    27121 atgggcaggg tggtaatgct ggaatggaac ataaattata ttaccataat tcatttattt
    27181 ttgctgaccc taacgaagcc tatgtccttg aaactgctaa caaacactgg gttgcaataa
    27241 aagttgatgg tatacgcagt atttctaatg gattaaccat agaagaggaa taccactatt
    27301 catcaaaaaa tattgaggat tatgcccgca aaaaaggata tctaaaaaaa ggcaaaacct
    27361 ttaactttaa agagtgcttt tctgattggt tttatacata cttttcacga tgtgctgcac
    27421 ggcaatcacg gacaacacgt tgtgcaatgc aacattctgg atccataaca cctcaaagta
    27481 tgtttgcatt tttacgtgat catggcagcg accatgaata tatttcacca gataaaactg
    27541 atatgggaac agtgtgtatg catgcatcac tacttcctac ccgtccaagc caatccgttg
    27601 ctgcatttgt tgcccatctt cgcaaagaca ctccagtctt ttgggctact ggctcgtcag
    27661 gaacctgtac aagcgtcttc atgccatttt atcttattgg aaaagaaata gattatttcc
    27721 cggcaaatga aacaagcact tactcacctg acgtgtactg gtggatgcat gaaaagattc
    27781 accgtcaggc cattttcaaa tgggattatt tccagagaac tatcgcccct gctatccata
    27841 cacttgaaga tgaatttatt aaaaaagaaa atgaactgta caaaaagaag aataccagta
    27901 gccagaagta ttttgatttt tcaaaacaat gtgtagaaaa ggtcattgaa cttcatgatc
    27961 gctgggataa ggaactgaaa gcagccccgg catccccaac aaaattgtcc tttaagctat
    28021 tctggaatgg acagacaaaa aaagccatga tacccctgta attacagcat ggggaagata
    28081 tatttttcta tcagctcaaa gttcttttta acgttgcttt ttccaaccac tatatccatg
    28141 tgcccgggca acaggtattc aatatcaagt tctttcattc gttcaatact ttctttcagc
    28201 aatgcactgc tgccgccagg aaaatctgtt cggcctacac tttccttgaa tatcaggtca
    28261 ccacatacaa gcactttttt tgattcccag tataaacaaa ctgaacctgg tgaatgccct
    28321 ggcgttttat acacctgcaa aacctcattg ccaatcttcc aagggccttc ttccagctca
    28381 atgtcaaatg taaattgtgg aaatttcatg ccaaacatgg caaaaaactg agggccaatt
    28441 tcatttaaaa aagctatctc ctctttatgc attgcaactt taggaccttt gcccataaac
    28501 tgctggcatc cttcaaaatg atcaggatgc gaatgggttg ccacaatata cgctattttc
    28561 tcagtatcaa ttccatcctt tttcatatcc gcaaagcgca tatccacata ctttgcaagg
    28621 cctggatcaa taaacatgtt gaaaggatca cctaaataaa atgtattgca gttattttca
    28681 aaaaccccgc gccatatata tgcatacaca ccatcacata ctttcataca ttactcctga
    28741 atctgtgaaa aatcaataag tgtggtaaat agtgtcagga tatagtgcaa cattcccaac
    28801 ctgttgtcgc gaatcgaagt gttagcatcc atcaccatga ctttatcaaa atatgtatct
    28861 ataatctctt taccttcaat aagcaaaagg aacaattctc tatacttgtt ttctgcaata
    28921 tattctttta tggttttttc cttattgttg aaaaaactat ataaacgttt ctcttcatct
    28981 attatcagcg catcgggatt aaatgaaaag acataaccag gattcttttt aacaaatgcg
    29041 ctgacaatat tgttcatgcg tttgaatgca atcaaaagat caccaaaacg cttatcctta
    29101 cggaattcat gaatgctctg agccctgaga aacagctcat agtaatcata acagccaact
    29161 gctaaacatg cattaacctc atcaaaggca aactgctttt catataaaaa tgtcttggct
    29221 ctgccattaa taaactcaag tattggttca acatgcttat ccccattact atattgcgat
    29281 gaaacttccc tgaatacgtt tgctaaatca agatggattg ttcgttcaac aagcatatcc
    29341 acaattgcag cagcttgccg tcgcagtgca tatgggtcgg cagaaccttt ggggatattt
    29401 ccaacagaaa acgaaccaaa tatgttatca attttttcag ccagcgaaac aacaatactc
    29461 acaatatgtt ccggaagcac atccccctga aaacgcggcc tgtactggaa atcaatagcc
    29521 tgcgccactt cttcatcttc gccatcaagc agcgcatata ttttgccaat ttgcccctgc
    29581 agggacgtaa actcaaacac cagtgctgtc gtcaggtcag ttttggatag catgacagca
    29641 cgctgaatct tctgggtaat ttgcgtatta agcgataact cattgcaaat aacctttgct
    29701 attgtttgca tcctttcaac tttatcgtag atagaaccca actccttatg gaacaggatg
    29761 cttttaagca tatccactct gcctgcaagc gggactttac ggtcttcttc aaagaagaaa
    29821 cgcgcatctg aaaaccgtgc tctaattaca cgctgatttc cctttacgat atgctctgtt
    29881 ggtgggttat ttgatataac taaaaattta ggaagtaaat tccctttact atcgcgcaca
    29941 gcaaaatatt tctggtgctc cttcatctca gttatcagta cgatatcagg tatagataag
    30001 aatccttcat caaagctgcc tattaccaca tatggcattt ctgttaaaaa ggtaacagtg
    30061 gtaagcaatt catcatcctc aacaagcaca ccacccaatt gttttgctgc attttgcaat
    30121 tgttcatgaa ttatagcttt tcgcttatta tgatcaacta tgaccccgtt gttttccagc
    30181 gtgggaatat actcgtcaca tcgtgataaa ggtatcatac ggttatactg tatataatga
    30241 cctcgcacct tattgtcaaa atcaatgcca tctattgaat agggaaccac tgcatcatta
    30301 aaaagtacgc atatgtagtt aactggccgc gggaatgata cacgtttaac gttccagcgc
    30361 atccgctttg gaaaatttag ctcattaata atcttttcaa taataacagg aataattgtt
    30421 gtagcaggtt gtgcttcaac tttacgcttt gcatagatat aatcaccttt gctggtcttc
    30481 tgtatgtatg tatcctcaag tttaatttta tttccttcaa gaaaaccggt taatgccctg
    30541 gttgggttac ccctgtcatc ataggctgct tttaccgaag gacctttgag ttcctcataa
    30601 tcttccgact gactatcggc catgtcatat accagtattg caacacgacg tggtgttgca
    30661 tacaccgtac acttcccaaa tgcaatgcga tatgactgta aaccttctgt tacaattttt
    30721 tctacctgtt caatagctga tgggatatac cctgctggaa tttcttcagt gccaatttcg
    30781 catacaaaat tacagttagc catacttatg tcctgtcagt gaatatattt ggtattatat
    30841 tatttacgtt tcttttttaa ctctgcatat ggattaatgt gctgcccttt acggattatc
    30901 tcaaaatgaa gatgtggtcc tgttgaattg ccggtattgc cactttttgc aataatctgt
    30961 ccctgcttaa catactgccc ctcacggaca gttatttttg aaagatgccc atataaggtt
    31021 ttatcgccac caggatgagt aaccactacc accttgccat acccccccag ccagcctgca
    31081 tagcttaccc ggccatattt gcttgcacgc acccattcat aattagcccg cacatcaatc
    31141 ccctgatgaa actggctttc accatagaaa ggatccttgc gccacccata accgctggta
    31201 atattctttg tgtgcacagg ccataaaaat cctttaaaaa tatccagtgg ttttgcatcg
    31261 ggaataaaaa tgtcctgccc aatatttata aaatcaggat ttgcaaagct gttttcttca
    31321 acaatcttgt ttattgccac attatatttt tttgccagag cgggtaatgt ttgaccacgc
    31381 tgtacactta ccagcagacc atctttgttt ggtatcttta aaacttgacc tacatgaacc
    31441 aggtcatacg attccagcct gttgcaaccg cagatagtat ccatggatac gccatacatc
    31501 tgcgcaatct cacttaatgt ttcacccgat ttcacagtat gctgaacaat ctttagcggt
    31561 tttttcttat caggttcttc aaagtctttg cttgcaattg cttttttctt tatttcatca
    31621 taccatggag cctcagaatt gaaaacatga tgagagcgga cataccctgt aaacagtata
    31681 acccctatca caatagttcc acacactaaa gctatgcttt taattttttt aaatggtata
    31741 ctcttaataa tagtagtttt tgtgcccctg aatataataa tgatctcagc agacaaaaca
    31801 gtaatactga gacttccaaa cgaatagcta tgtaattgcc agtgcttcat agtgtaatta
    31861 tcggtatttc tgtttcacga atcatctttc ctggcctttt ttcccaaata tgcctgtctg
    31921 tgcctgtggt gcccctgcat acgattcttc aataagtttt tttacatctt tcttttgccc
    31981 gccagtaatg gtaaccatgc cttgtgccac cacaccacgc tgcacagtaa gaattggcgt
    32041 ttcaatatca cccagcactt ttgctgtttc taaaagttta acttcttcat cggcaataat
    32101 atttcctatt acagttccag caaccacaac attctttgca tgaatattgg tttttacctt
    32161 tccggtttcg ccaacaacaa gttcctcatc agtttttatt tcaccttcaa atttaccgtc
    32221 aattcgcagt gacccgctga tataaaactt tccttcaaaa acagaacctt caccaatagt
    32281 gctgtttata ctatcacttt tacctgccat actgttaaca ccccacgatt ataaatttca
    32341 tcaccgttac aatttacacg tactctcaga tttttgcaat tttatttcaa ccatttttta
    32401 ttgtaaatca attaatcccc cggtaatttt tattattgca tatttttagc aattacatat
    32461 tttgtgcgat agttactgtt gaatagcctt aaggtttcaa cgtgtaccaa taccaataaa
    32521 taaagttttg cacaatagac accctgaata cggattgtat gactgatttg atgtaaggat
    32581 gtcaataaag taattgaaac accaggtcat ttaagcgaac gttctttact nnnnnnnnnn
    32641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atcccgatga aatcgggaat ttgaaataac
    32761 agaagaagtg acagtccgtg tattttaatt gaaaaaattg tgatacggta ataatgtaac
    32821 gttattgcgc acctaatagc atatacaaat gagttataat tgattaatct atgaatacaa
    32881 atactatcac actgtgtgtc aatgagatat tctattcgct tcagggtgaa actactcgca
    32941 caggtttccc ttcgcttttc atacggcttg caggctgtaa tcttaactgc aggtggtgtg
    33001 atactcccta tgcaaggact ggattaccgg taactatcgc ccacattata caaacaatag
    33061 agcaatatcc tacactaaat cacatcacta ttaccggtgg cgaaccgtta atgcaggaac
    33121 acacattgac acttcttgat attctctgta gaaaaaatta tcccctgcaa ctggaaacca
    33181 acggaagcat ttcagttgaa tacgtgccac caaaggtcca taaaattgtt gatgttaaaa
    33241 caccttcaag tggccatgct gattcattta acatttctaa tatacagtat cttaaaccgc
    33301 atgatgaaat aaaatttgtt attgcagatg actatgacta taattattct aaggagttta
    33361 tgaaaaacta tctggctaat gtaccctgtg tcataaattt ttcgcctgtc tggggcacaa
    33421 tggagccaca acaattagca gagtatattt tacatgatag acttaacgtg cggcttaata
    33481 ttcaattgca taaaatactc tggcatagtg aaccaaaata tttttaggag gtgctatgga
    33541 ttacgtaggg catattatac ggccaccttc agaagcatac agcatgataa ttcaggtaac
    33601 cgttggctgt tcacacaaca tgtgcacatt ctgcggcacc tacaagggac gcaaatttta
    33661 catcaaggac ttgaatcaaa taaagcgtga tattgacgaa gcatcgcgtt accacttcaa
    33721 gcgcgttttc ttaaccgatg gcgatgtcct gattttgcca acacaaacac tgcttgaaat
    33781 aatttcctac atcaaaagta aaaatccgca cattgaacgc attggtgtat atggcaatac
    33841 caaagccata ttaaaaaaat cattatctga actgcaggaa ttaaacgctg cggggctggg
    33901 tattgtgtat cagggtattg aaagcggtaa tgaaacagtg ctgcgcaaaa tacggaaagg
    33961 cgccttgcca cacaaacagt tagaggcatc tgaaaagatt aaacaggcag gcattctact
    34021 atcccagaca gtgctgcttg gcattggtgg ccttgagtta agcataccgc atgccattga
    34081 tactggcaag catttgggtg ccatgtcacc agactatgca tcagcactta ctgtaatgct
    34141 tttacccaac acaccattgt accgggatta tgtacaggga aaatttgcac tcccggataa
    34201 atttgggtta ctacgcgaac ttaaacttat tgttgagcat atggatgtta aaaacacctg
    34261 ctatttcaca tccaatcacg cttccaatta tctgcccatt aaggcaacat taccgcaaca
    34321 aaagaacgac gtcattcaat tgttggatga aataatacaa cggcaggatg aatcattatt
    34381 aaaacctgaa tttttacggg cactgtagga gggaatcatt atggacaaat caatggaaga
    34441 cgctatccgc tttatcagct ttgaattgca ggataatcca ggtgctgata ttgccaaact
    34501 tattgaaaaa gcaagccagc agtttgactt aacgccactg caaacagaat ttttagtaaa
    34561 taaatttatc ttaaataaat aataaaaagg gcttgctttc gcaagccctt tttatgttat
    34621 tcttcttcac ctgtcagttt atctaatagc tttttctgac cacctgcata tactgctacc
    34681 ttttcgcctt ctaattcaac cttgccatca tttgccagtt tctttctcca gagcactgta
    34741 ccatctaatt ttgcgcagaa gatataatca ccagaagcta tgtaaatttt atcctcggtt
    34801 tttgttggag ggttaatgac tttaccttcc cattcaacac ggccattctt tacaaaatat
    34861 atttcatagc gtgttacaag taactggcct tcaggctgag gaatctgccg tgttttctta
    34921 acaacctttg caatttcttt taccacttct tttggttttt cttcaatttt tattatttcc
    34981 tgcttttctt ctttaaaatc ttcaggttta aacttctgca cttcctcttt ttttattacc
    35041 acttcttctt ttgcagcagc tacaactttt tctatggcct gtggggtggc ttcaccttct
    35101 tttttcatta cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaactgcaa
    35221 gacctagtat gagtgaatat atcagaatcc gtttcatcac acccccctat aataaaaaaa
    35281 ttatatacca gattatgtaa taccttgctt aaaaaataac catttaacca ttatagtata
    35341 ctattgtttc ataaataaat gcaatttttt tttattatac ctgaaaatta atactttagt
    35401 aacaaaaaga tataaatcat acaataaatt aattattcta taccaatatt ggtgacactg
    35461 gtatagagat gacatttcac gtctatttat gcactttgct gtattaatat atattttcat
    35521 acttgactgt taccagcagg tatactgttc tatcaccatg gatcgttcta agctcataca
    35581 aatccttcac ggcattatta ttgccgatgc tcttgcaagt cctcttgata ccttgagtgc
    35641 tgagcatata cacaatgttt ttggcacaat acacgattat actgacccag cgcctgcact
    35701 taaaaataag ctctatctgt ggaaaaagcc agcactctac actgcccttt cacagtattg
    35761 tattcttctt tcagcactca atgctcaaca agatacctat gtgcaccagc acacctgtaa
    35821 ctttatatct tctttaggcg atagttcccg tatatttcgt catccccagg gatttttaca
    35881 acacctgcaa tcaccgtatg atgcccccac tgccgaattg cttatctgta tccctgctct
    35941 attcttttta cataaacaaa accctatctc aattatacat tttattctta gtcataacaa
    36001 gaatgcagct acatgtgcag catcagtatt tgtatatatg ttacttgaaa caataataat
    36061 gcgacagctc atagcattgg atagtgttgt attaaaacaa actgcagaag atgcgtatca
    36121 atttgttcag gaacactcag cgcttttttt taacaacggt ggcaatccac agaatatcat
    36181 tgaagctaca aaagatcttc atgatttaat catcaacttg ccacttgaaa gtgatgaaac
    36241 acactacacc agatattgta tcccctttgc aaacaggtgg tcaaaaaacg aatatacccg
    36301 gcttaccgtt aatcatcctt ttacattgtt gccattagct ctgtactgtg ttcatactgg
    36361 tcaaaaagac tctttactct attctgccat ccataaaggc ggcaagatat cgttattgac
    36421 tccactggtg gctttgcttg caacggccat gtatggttac gaggtaattc ctcaaaacct
    36481 aatggacacc attgtcaata aaaaaagaat tttgcagttg ttagctttat tacagagtca
    36541 ttccatttct ataaactatc tcacagattt ttttcaaaat gaagcaaaat taacacaaaa
    36601 agagttagaa gagcgtgaaa gcaaattaaa acatatcaaa tccaaacaaa caggaaaaaa
    36661 caaagataag gatacataca catcaatgac ttaccatgtt gttgaaagct ggacaaagtt
    36721 agacaaagca aaatggaaga aggaaagaag aagaaaaaaa taatgtattc ttcctgttct
    36781 ttcagtgaca tagagcaact atattgtatt aattaactga ttttattcaa tataatgttt
    36841 taaatactgt accataagct ctttaaaacc tggcaatgtc agccatcgtc cgtatgccag
    36901 ggcaatgata gccaattctt caccacgttg tgctaccacg ccaccatacg cagtagtaat
    36961 ctttttacca tattcaccac gtatatcatc acagaatcca attatatgga atttatcagt
    37021 attatacact ctttcataaa aggttatctg caattcaaca gtaacacctg cctgccatac
    37081 tgaaagaaac gttgaccacc ccattgtttc atctaacagt gaactgagca caccgttgtg
    37141 aatagagcca tctggagcct gtgcaaaatt atttttacca tgtaagtgca aaatcagata
    37201 tgttctatct gtggtatagt cagttatata aaatattctt tgatcatgat ctgaaattac
    37261 agtattagtt aacttagtag acatattggg aataataaat ggagtggaag catccatatt
    37321 attttttagt attgtaatta tttcattacc atactcatga acatgccctt cgctgtaacg
    37381 acaattacaa tatggctgct gcaaagattt tccaaatgat ccaaaaaaat atcgtgcatc
    37441 acctttctgg acaatacacc gtaccatatc accacatttc aattgttcac cagcaaaacg
    37501 gaatcggtat tcatatggaa atactgcagg gaattccttt gcaatatcaa tcagttcaat
    37561 aaatgtggtc attgctaagc cgccatgagg tataccaggc cacccttcat gttttttttc
    37621 tatttttata tcagctacta tcgctaaatc ttcaaaacga taggatctga atataaagcc
    37681 gttttgccca caatagtaac aattgttatg tctcatattc agtatattaa ttactataca
    37741 atcccaccgc taaaagcagt ttgtgtatac aatgtataaa tcattaaaat ttttaaagct
    37801 gtaaatactc aaaacaatca acccttaaga aagaaaaatc atccttattg taaataacaa
    37861 ctattttttt aatttatata aaatatttga caaaaatatt ttaatcagtt aatttacgta
    37921 ccaggtgtta ttttcaattt agctaaaata ttctggaatt aactaagtca tacttttaca
    37981 ctatcaggga tggaggaata tatgtttttt attaaaaaaa ggaattttct tgtattagtg
    38041 tttatattaa tatgcactac tgcagccatt gccattgatt ttaacttcaa accaattatc
    38101 attgaagaaa tatcccgata taatgaggat agaattgcat atcaacacgt tcagaaacaa
    38161 atagctccaa atatggacaa ttctttctcc gcactgttaa tagtaaagga caggaaaata
    38221 taccttattc aggatggcta tgacaatcca gaattaatca ataccaaacg tcttcagatg
    38281 gagatggaaa caaaacttat aggcgatctg tgggaaaata aaataaataa taaacctgat
    38341 tacgtacgga ttactgaccg taaagttgag cttttgaaaa atttcagtga agattttgta
    38401 tccaaaaatt ttggtacatt ctttttaaat gtccgcaatg cttttattaa aaaacatgtg
    38461 gaagtcttta aaaaacttat ggtagaccga aaggaatcag gtcttattgt aacctataca
    38521 ccacttcctg ttcctgcata cctcaatgca cctgaaacac caacaaaata taaaataacc
    38581 gtaagtggca agactattga tgaaaagcta tactatgctg aagatagtga tggcgatggc
    38641 attaccgaaa ccttcatggt caatagtgct gatggcttta actggggata taaatcaggt
    38701 gccaatatta tttttatata taacaatctg gatgaagaaa ttaagggttt aattggccag
    38761 ctatgtaact gggcatatta tggcacaccg gaagaagaaa aagaaatttt acaaaatttc
    38821 cccaaagaca gcgatatcat aaatgaattc aaattagagg taccacaaac aacaaaataa
    38881 acaaacatgt aggggcgaat ttaaaactcg cccctacatg ttacccctgt tcaactatat
    38941 tttttgtgca attttttcaa ttcttccagt aaactgtaat cattatcttt tataccaccc
    39001 agggaaactt ttggtttgga tgttggaccg tggaaatcag atcctgcagt tataacaagg
    39061 ttatgttgta cagcaaattc cataaaaaat gctatatcat gctcattgtg atatgaagag
    39121 atagcctcta tccccattat ccccatcgaa acaatgttac gtaaatgctc ttccccattt
    39181 acaaactttg ggtgagctaa taccggtata ccacctgaag acttaatgac atcaatccct
    39241 tcttttgttg ggatggaaat gaatggtaca taggcaggca tattttcaga aaggtattct
    39301 ctgtagaaaa acattagtct attatcagat ttttttcctt caaaatattc ctgtaatctc
    39361 ttatcatttc tgttttcagg attttcaaac acttccagca taatagctgt agcagttggg
    39421 ggacggttat ttgctttttg ccacacatta tcttcattca tagaaaaacc aatttcttgc
    39481 aactttttaa ttcttttttt tgctatctcc cacctgaatt gccctatttg tttcaatgtt
    39541 gcgtttaatt gtggattttc atgtatataa taacccagaa tatgttgctg tgaaccatcg
    39601 tcatatattg tggttaactc aacacccggg atgtattcta ctccatatat gtttgaagca
    39661 ttcagcgcat ccgggattga ttctattgaa tcatgatcgg taatacttat tgcggataaa
    39721 cctttctttg cagcaagttc aaaaagataa tctacagaat aatcaccatc ctcgctatag
    39781 tgagaatgta tatgcaggtc aatgcggtga gccatacaaa aattgtatca cattattttg
    39841 ggtttttcat gataatgaga tttttttcag gcagtatatc aagtggattc agcggtatat
    39901 cagcacaacg aatctcaaaa tgtaaatgat tgccggtaga acggcccgta cttcctacaa
    39961 gggcaatgac ctggcctttc tgaacataat caccaacttt tacaagtaac accgaattat
    40021 gggcataccg ggtgatataa ttatccctgt gttcaagtgt tatagaattt ccatagttcc
    40081 cgcaatatcc agcatagatt actttccctt ccatggctgc ccgtacaagt gttcccttag
    40141 gagccggaat atctactcca ggatgaaatc ttccccagcg tcgaccaagg cctgatgtaa
    40201 ttttttcagg atcgccaatt ggccatataa attcattata tgaaatgttt acaggctgca
    40261 aggagatacc tttttccatc aacatttgaa tataagcatc cgaataaggg acaaaaacat
    40321 atgatttttt aaactttttt tcattaatac tatttattac atatacctca tcaatagacg
    40381 taccaaactg ttcagcgtaa gcctttagct gtttgagggt gctaacttct atccatttac
    40441 cacttttccc gttataccgc tggatatgtt cattatctat gaaatagagc tcattggcaa
    40501 atgttactgt ggtaactata acaaaaaaaa caagtactat tttctttata cccataacag
    40561 atcaatcctg tacatatcat tttttacaac aaccactctg ggagttagct tactatagag
    40621 attatatgta tagaaaatta tttataagca aacagtaacc ttattatagc ttgaatttta
    40681 ctgactaatt gtcaacaatt tttttaccat gctaacgata aaactttttc atctgaataa
    40741 ttgcttttaa catggtgcct ccgtaaacaa aaattatctc ttgtaactat attggggcca
    40801 taatcagtag agaacacccg tttataccct gcatctttac acaactctgt taattcaatt
    40861 gaatagcggc cataagggaa tgcaaaataa tcaatagttt tatctaaata caattcaaga
    40921 atcttttttg aaagataaat ctccctgtat aatgcatatc tgctttcaag tgttccctgg
    40981 ttatatagtt ttaccaaatc agcatgacta tgtgaatggc attgtatgtc tatgttattg
    41041 ttctgcatta tttgcaactt cttccatgtt aaattttttg ttccgcccat cgcgatagca
    41101 tctatatata caaaaagggt aacaggatag ctcatggaac gtgccattgg cattaacatg
    41161 gtatacatgg agtaatagcc atcatcaaag gttattgata taaccttttc accaaatgga
    41221 ttatgctcat caatatgcga ttccagttgt tgcagtgata ttaccttaat accattgttc
    41281 tgcaattgtg taaactggtt cttcagctta tcaaaaggaa gagaataggg gcctttgcca
    41341 ttgatgttat ggtataaaag tattggaact ttatgaagcc gggtaatatt tttatgagct
    41401 atcgccctgt aaacttcttt ttcattttct atatatgtat cagttttata gtatacacct
    41461 atatacaatg acactacaag aacacttaca aaacttatac ctataagcag attatcaatt
    41521 tttatcatag tttaatcaat atatcagtaa gaaaccgtgt tccataccca tttaataatc
    41581 ggtgtagcaa ccatgttgtt ttacccataa acccggggac gatcataaat ttccttttca
    41641 agattgcttt cataattgat tgggcgatag tatctgctga aagaacccct gtaatttttt
    41701 taatacgttt tgctttt
//
LOCUS       DAORYL010000016        41533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_749_length_41533_cov_13.236800, whole genome shotgun sequence.
ACCESSION   DAORYL010000016 DAORYL010000000
VERSION     DAORYL010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 41533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41533
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_749_length_41533_cov_13.236800"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..239)
                     /locus_tag="PLV81_04940"
     tRNA            complement(156..239)
                     /locus_tag="PLV81_04940"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(203..205),aa:Leu,seq:caa)
     gene            complement(252..668)
                     /locus_tag="PLV81_04945"
     CDS             complement(252..668)
                     /locus_tag="PLV81_04945"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017862332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOM09478.1"
                     /translation="MIEFEDKTCFGCGKSNERGLKLHLKFDPDTKTAYGEFKADQTLE
                     GPPNIIHAGIIASILDETMMTVNKYMEYIALTGEITIRYLQPAFIEENLYIRGWFVKK
                     NKRVIENRAEIENEMGKIVARAKAKYIEVEEIPQPE"
     gene            complement(761..1042)
                     /locus_tag="PLV81_04950"
     CDS             complement(761..1042)
                     /locus_tag="PLV81_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09479.1"
                     /translation="MKNMHDKKVGAFLVENGIISEEQLQEALELQRDNPERLIGEILV
                     TMGVLTKEELVMALEMYMMTTDAMPEHVDEWLDQDEIDLLMEKIKNESK"
     gene            1244..1774
                     /locus_tag="PLV81_04955"
     CDS             1244..1774
                     /locus_tag="PLV81_04955"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HOM09480.1"
                     /translation="MAARKNASFVGVAVCKKCHESEAIGNQYKSWQQSPHAKAYLLLK
                     QEKALSLAKELSIENPSDNEKCLACHTTGLGKNPALWEDGVGCESCHGPGSMYNSYEN
                     HVALGNREVAYKKALSFGMYPILGTDGIKAREKVCRSCHNEKRPCFPKDPAEQQHQYL
                     PLQLIADFAFPHRLRR"
     gene            complement(1775..2692)
                     /locus_tag="PLV81_04960"
     CDS             complement(1775..2692)
                     /locus_tag="PLV81_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09481.1"
                     /translation="MHSFVLISNNTAQQSELAKHSVTAECTSEDFSVNVLKQNRDIAC
                     IIDFNSSGMAVQYESLIKEIVTANLPCIGICSEIQSLKKTLIRYGITAVFRPSQYHYI
                     PLFFKQYTPAITGTIALIDNNVFNTYGLSTVIQAFGYQAIVVDSLEACCDIHNVMDMV
                     CINCSQVSTHEIATKYIAGKLPKKNALVLYKSEESDIFIHDIIKLHRIAKVIYTLEEV
                     YALLVQLMFRQQLHSLLYSLYETSDMQRSTTAYKGSLRQLYLETGMEIFALPAITHTE
                     TIELFRDNTERMHTILAKAAGFSWLSDNE"
     gene            complement(2698..4239)
                     /locus_tag="PLV81_04965"
     CDS             complement(2698..4239)
                     /locus_tag="PLV81_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOM09482.1"
                     /translation="MDVMRNVLAKYSDASFIMQQRAKTLSYFLTVCIVLVLFFLVAQN
                     LMATRPLFSFINAVLCDILIFLTIATLLLRAGNYSWAANISVIGSVFVLGLLVNFGTM
                     AHNEGIILTNYQFLIFIIFSALFCSRRMVITVAIVSLVFAIISFIRTDLISGKVKTVA
                     IVDFTFELIIITAISYLLLRLMDTTIQKLQEELENRDQLMRIKDLLEAVKDISLKLAV
                     ASHELSDTSKNFSVNAQNQAALAEEITATIEEISAGVENVSNSANYQYDSINSFTRRT
                     EELSSLVSRMEQEINNALQLTASIETHARLGAELLNGMDTSITNIHTSSGEMTNIVKI
                     IKDISDQINLLSLNAAIEAARAGDAGRGFAVVADEISKLADRTSVSVKDIESLISAND
                     KEIQKGMTSVQQTVDTISGIIKGVNEINAMVKVLADYMKQQLEVNVIVTKESGQVKVR
                     SEEIRNAAEEQKNASGEIVKSVAGINELTQANASGALKITESADTVLSMSDILKHRVE
                     ALEIV"
     gene            4576..5274
                     /locus_tag="PLV81_04970"
     CDS             4576..5274
                     /locus_tag="PLV81_04970"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HOM09483.1"
                     /translation="MAGSFDFSQFINDSKATVLSPKDYFSSMAKEGGLVEPIIKAVLY
                     GVISGVIYFIFTLLNLSAYTMFGTGAAMALIGPAIFAIIGLFIGGVIMLIISAICGGN
                     TAFEANARVVAALMVLGPVQALFSFLTSISFYLGLIVSALIALYGLYLTFIALVNALN
                     AKENVAKIVIGILAVLYVLSLYGSFKAYRFAGHMGEQMLEQTEKLSSEQQKALEQLQQ
                     LQKSLEKLEQEQNE"
     gene            complement(5317..5931)
                     /locus_tag="PLV81_04975"
     CDS             complement(5317..5931)
                     /locus_tag="PLV81_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015190871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOM09484.1"
                     /translation="MIHENVKTDFVSREYTPVIDASAYVHPLAAVIGNVILGENVMVA
                     PFASVRGDEGQPIYVGDDSNVQDGVIIHALETEHHGHPVEKNLVEVSGKKYAVYVGTR
                     VSLAHQVQIHGPAFVGDSSFIGMQSLVFRARVGKGCVVEPKSLVMGVTIPDGRYVPAG
                     SVIKTQHDADNLPVIDDSYPFKSLNDGVIHVNVALAAGYKVKKI"
     gene            6078..8369
                     /locus_tag="PLV81_04980"
     CDS             6078..8369
                     /locus_tag="PLV81_04980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM09485.1"
                     /translation="MFKQFLHKHGISNITLLATESPLHQRIVSYLSRNHITYTVIATD
                     DPLPDTHSLLILPLSMHTITPVIDILQKMLTSTYTPLLLFATDFPITTRFKKRFSIAG
                     IIGILELTERPHFITLETQSLQSNPTASLNALIYTTENLISRNIYKLLSQNILYNALV
                     KLHPSVVCITTLQGTIIYYRAHRQYFTRNNNIKKLLYGKKIQELTTQPEKIEHLFSLI
                     DRNIEQRESEITFRMNNTDTTLLVTGEKIKLYGDLEFYLLIGINITTQRKAEDMLISS
                     ELRYRTLVENLPDGLSLSKGDMLLYANPQFFKLFNFTTETLITDVNLKSLIHPDDWAA
                     LYELLQKPIGNVATINVKAYTATGKPLILELSVLSFMGDSSLYHQIYWHDITQTRQLW
                     DKILQSERLSAIGEMASGITHEFNNILTSIQGYVQYTLNNPDDIEANTKAFKVIEKMT
                     EQGHAILKNLSMLTRKDITNKERHNIADLINDIVRIQEKILIKDNITLIKKFVKNPVC
                     YVDAGLIQQVFLNILLNAVHAIRPKGHGTITITVDESNGNAIIKIHDTGTGIDPKIQD
                     EIFNPFFTTKTGTHNIVGTGMGLSISKNIIEQHNGRIWFHSIPDKETEFIIELPIYKT
                     AYLPKELEASSQPKDLSNLTVLIVDDDDAIRDLFKTLLQSLGISAITFATNGYEGYSL
                     CKENQYDIVFMDVSMPVLSGIDAYKMIKDENPSQKVVFITGIFYEDQIKELVDKEQAF
                     GYIKKPFDIKEIKSLLFTIASHQ"
     gene            8421..9287
                     /gene="mtnP"
                     /locus_tag="PLV81_04985"
     CDS             8421..9287
                     /gene="mtnP"
                     /locus_tag="PLV81_04985"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOM09486.1"
                     /translation="MKPKIDVGIIGGSGLYQIEGVTILEEVKVRTPWGMPSDAITIAE
                     VGGVKAGFLPRHGRGHFILPHEINYRANIASLKMLGAGQIVAFSAVGSLKERIKPLDF
                     VIPNQIIDRTKSRISTFFGDGIAAHIAFADPFCTRLHELILVAAQKLNIPMHTNETLV
                     CMEGPAFSTRAESHLYRSWQAGVINMSTLPEAKLAREAEMCYAVICMSTDYDCWKEDE
                     EHVTVDMVVNNLNKNASNAKALIKELIPLLTKERDCGCKEAIKYAIITDPKKQSSKQK
                     KKLKAILPNYFS"
     gene            complement(9284..10708)
                     /locus_tag="PLV81_04990"
     CDS             complement(9284..10708)
                     /locus_tag="PLV81_04990"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOM09487.1"
                     /translation="MKTYTVTLAIPPRVAKEIKAICTTLTSNGHECYLIGGSVRDLIL
                     GIPVYDYDFATNAHPEDVMKLFKKVVPTGIKHGTVTVLMHYAQYEITTYRADGKYTDG
                     RHPDTVVFSKTLKEDVERRDFTINGLAYDINTSKIIDYVGGMDDIEKKIIRTIGDPLQ
                     RFSEDGLRPFRACRLAAKLHFSIHKKTLEAIPQVLEIAKKISVERVRDEIKKMLEADK
                     PSEGFEYMRVTGLLDLYMPELSQGFGMEQNKYHMYDIYYHSIYSCDAAPKEKPLVRLA
                     ALLHDIGKIPTRREGSDGDYTFYNHEVISSKIARKIMRRLKFSNDEIEYVTNLIANHM
                     FHYTNDWTDGAVRRFIRKVGLDNLDDLFLLRLADRCGNGMRDGLPEPIKKLKRHIQRV
                     IEKDNAFTVKDLDIDGYILMEHLKLKPGPIIGKILNELLEQVLDNPELNNQETLLQMA
                     HELYAAMANDTTATCHDSTQHHIV"
     gene            complement(10705..12933)
                     /locus_tag="PLV81_04995"
     CDS             complement(10705..12933)
                     /locus_tag="PLV81_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOM09488.1"
                     /translation="MKIFTISELIGTMKQFPLLQKVSPVEAVKSLTFFKDVPEAVLQE
                     IVDGIFIHQYAKDEIISRHGRYNEWLYVVLSGEISVFIITPDYTKLELYALGPEDFFG
                     EDIVIRNEPRESTAIAFTDSILLAIGKQELTKIIASSAATYEKLNNAFLQRKMRNNLR
                     SIPIFTHLREEVFNEILDVVRLVHVKKGDVIFKQGDVGDALFLIRKGDVSVYRAMNKN
                     EELISLLAEGNFFGEMALVLGEPRNATVIANDDCELLKIDKNDFDSIIARHVDVYNTI
                     QAVALERVTGHELFDSNEAFISKKLIELNRAINKHIDVIAQCTFETPKGSALLATLPG
                     SRYPYVYPRDSACATRMLYRIAMSRLRSKDIAFRLLAGIAKFIYNCQRDDGYWGQRYG
                     LDAADKSIYKQEDNVAHGVSILCRYLLAAKNRGHIPHDNEAYIEAIYKGAMFAVKRYY
                     RNEIHLFYSTTSIHESAIEEGYSIWVNYAYYCMLLLIEQVAKEFNCETKFEKAFVLKE
                     GFGTIVKNVFTIGDRLIRRLKPDGEADLRPDITLMSPFFFGTGIVPDIFKADEIFINT
                     MKFLEDTLWDPDLGMLQRYLPFIEDPNTHVHAGNGPWVQYTAMLAQYYYFTGNVEKGD
                     TIMDIIDSYSTDGYLCEHLTTPDRFHEFMTLEWLPGSDVNKEFAPDIMVDGITYDLIV
                     EELNHMKNSYDQIKRRIETGSGRYLTFAIPLMWSHAEYAMALLLKTWRQLQDTGVKQH
                     IL"
     gene            complement(13020..14681)
                     /locus_tag="PLV81_05000"
     CDS             complement(13020..14681)
                     /locus_tag="PLV81_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09489.1"
                     /translation="MKKVNIKKYYAITGIIILSVLCIALTYDTTYQIMFTSNGLDLFN
                     VKKIKLQGNQSGFTIAKINYKESVNPLVTDLLITFDKDFPAMVKDDSGHYAIRYAQYT
                     YTKEDIENEGCAYFFHKDQRIEIQTAKNNWLTNCTDLGSFTIEAKLKLTKYSPLAVVL
                     SRIGYSGGKQGFEIVCVNDKLAIRFYKMFYKKDNVPVDVFCHRSPSLALNTWYHIAVS
                     FDRISGKLETMINGDVVETIFCTESGEPFVDVMVPRFVCQDIPVAVVGKHFYGYLDKV
                     RIAQRYILDLEKETEIAKTQFKAITNNQRDTINREGIITSPVYTFPDYGTMVTLFAWD
                     EKMPPSTYIWMEFRISDAFFKANDETPQWYRITNKQKNIYLHRVGNEYLRGKFYQWRA
                     HLIASPDGSNAPVILNIGLQYALDTAPQPPIFVTVDSVGNNMVALKWKKNVEYDILGY
                     NIYYGVKSKQYDGIIRTVNGKRISNELSDTNGYITVTLTNDVIEENRANDTKGFLTFP
                     VLKNNVLYFFAVTAYDSYKPDTMFNHESKHSKEVSARPDPGSDIK"
     gene            complement(14678..15115)
                     /gene="rpiB"
                     /locus_tag="PLV81_05005"
     CDS             complement(14678..15115)
                     /gene="rpiB"
                     /locus_tag="PLV81_05005"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012057395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOM09490.1"
                     /translation="MKIAIGNDHAGVELKQYIMQHFKEHNFINVGTDVTQSCDYPDYI
                     KLVAELVQKGEVDYGIALCGTGIGASIVANKYKGIRAALCTNEFMATMARRHNDANIL
                     VLGARVVGMDLAIHITAAFLQGTFEGGRHQRRLDKIAQIEIME"
     gene            complement(15130..16515)
                     /gene="asnS"
                     /locus_tag="PLV81_05010"
     CDS             complement(15130..16515)
                     /gene="asnS"
                     /locus_tag="PLV81_05010"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015225265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOM09491.1"
                     /translation="MKRMRIVDCLKSDIPIDDCIVAGWVRTKRESKENIFIEINDGSC
                     LSNLQVVVGVGDSSFDIAQRLHTGASVYIQGSLVPSPAVGQTWELKPNIITVFGDADP
                     ESYPLQKKRHSFEFLREIAHLRPRTNTMGAIMRIRNQVSYAIHTFFQQKGFLYVHTPI
                     ITTSDCEGAGEMFQVTTLDLHNLPMVNGAIDYTKDFFGAKAALTVSGQLEGELLAMAL
                     GDIYTFGPTFRAENSNTTRHLSEFWMVEPEMTFATLEDDCSLAEEFLRFIFKWVLEKC
                     QEDMQFFNERIKPGIIQNLELIVNSSFEIVTYTEAIEKLQQSNESFEFLVHWGMDLQT
                     EHERWLTEKYFKKPIIVIHYPKTIKAFYMKLNDDNNTVRAMDVLVPGVGEIIGGSERE
                     DRYDVLITRMKEMGLNPDDYWWYMDIRKFGSAPHAGFGLGFERAIQFITGMQNIRDVI
                     PFPRTPGNARF"
     gene            complement(16567..17796)
                     /locus_tag="PLV81_05015"
     CDS             complement(16567..17796)
                     /locus_tag="PLV81_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HOM09492.1"
                     /translation="MKISCIELYHVSIPLRHTFWPTWIPGYPQTHNRFTLIKLITDDG
                     IEGYSAGTAMGKEREGLGDLLAGYIMGTDPNDIDRVQSLLKQAGYLGWRNFWIEPAFW
                     DIIAKSKGKPVYELLGGNARPIEVYCSTGEMHDPHKRVDEILKLTERGFKTFKLRVKN
                     KELKDDIRHIEIISKGVGNKVKLGVDANQGWPVSIVDRVPDWDLKRAKEFVRACEANN
                     IVWLEEPLDSRDYDGNAALKRFAKKVKISGAELNYGWDEIKIMFEKDCFHIYQPDATF
                     AGGIAQVKKVIDTCHEKKREFSPHTWTNGIGFYINWNMVLSDTKNKLPLEYPLEEPSW
                     IPEFRDGIIEPIIPDKNAMLQPFTRPGLGFEIDKKLLSKYGKRYFRMTEFGLKIKVIR
                     EKGIKEALALKKRKEGQ"
     gene            18158..>18453
                     /locus_tag="PLV81_05020"
     CDS             18158..>18453
                     /locus_tag="PLV81_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09493.1"
                     /translation="MRFIFAALLFFLSVSPLYASNKVYVLVYHTFMGKKIKYDISIQE
                     FQKQLLDLRSHGFSFISFDDIKYRKVSGDKNILITIDDGHHTVLDAYYSVLKPLG"
     assembly_gap    18454..18553
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18554..19788)
                     /gene="gltA"
                     /locus_tag="PLV81_05025"
     CDS             complement(<18554..19788)
                     /gene="gltA"
                     /locus_tag="PLV81_05025"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOM09494.1"
                     /translation="MADKIPRQKMPQQDPAKRIHNFNEVPLGYDEKTAVLEATRCLQC
                     KRPTCVEGCPVNINIPGFIKAVKEGNPLEAFAVIKQTNSLPAVCGRVCPQEDQCEKLC
                     VLAKKGDPVAIGNLERYVADYEREKQHYTFTKGETVKGKRVAIIGSGPAGLTAAGDLA
                     RMGYSVTVFEALHKAGGVLVYGIPEFRLPKDIVQYEVDALKAMGVTIELSRVIGTAET
                     VNDLLSSGFDAVFVATGAGLPLFLDIPGENLQGVYSANEYLTRSNLMKAYRFPEYDTP
                     IVKGEKVAVVGGGNVAMDSARTALRLGSKEVYIIYRRSKKEMPARIEEVERAEEEGVI
                     FKLLTNPVRFIGDEKGRVKAVECIQMELGEPDASGRRSPIPVKGSEFIIDIDVAIIAI
                     GNGPNPLIPKTTPGMDTTKR"
     gene            complement(19778..20620)
                     /locus_tag="PLV81_05030"
     CDS             complement(19778..20620)
                     /locus_tag="PLV81_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOM09495.1"
                     /translation="MNKIIQNSKISENVGKAIIEAPYIAHHRKAGQFVIVIVDEVGER
                     IPLTIADSDREKGTITLYYQIVGTSTHKLSLKKEGEYVYDVAGPLGHPTDIRNYGTAV
                     CVGGGIGIAAVYPIAVAMKEAGNFCINIIGARNKDLLILEQELKAKSDQIIVTTDDGS
                     YGIKGFVTDELKKILDSKKVDIVVAVGPVPMMKAVSELTRRYNVKTLVSLNSIMVDGT
                     GMCGGCRVTVDGKKQFTCVDGPEFDAHLVDFDELMKRLNTYKDQEKESFEKHTCQLEK
                     YHGR"
     gene            20985..22583
                     /locus_tag="PLV81_05035"
     CDS             20985..22583
                     /locus_tag="PLV81_05035"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HOM09496.1"
                     /translation="MGNLSRPNSNDATRTANRSRSVAPQSGICSRCLDGCTGNCEIFQ
                     ATMRGRELLYPGPFGEITAGADKDYPVDYSHLNIQGYAAGAKGLLNPGPETARFPLVS
                     TETYFGTKNRVKLEVPMFTGALGSTDIARINWEHFAVGAAISGIAIVCGENVCGIDPG
                     LERDKNGKVTKSPEMIRRVETFKKYKGEAGDILVQMNVEDTNLGVAEYIISQLGVETV
                     ELKWGQGAKCIGGEIKVRSLERAIELKNRGYIVTPDPTIPAIQAAFKDGAIKEFERHS
                     RLGFISKEAFLQEVERLRALGAKRVTLKTGAYSQKELAAALRWASEAKIDLLTIDGAG
                     GGTGMSPWRMMEEWGVPTFYLQCMAYDMAQKMASKGMWVPDLAMAGGFSTEDHIFKVL
                     AMGSPYFKAVCMGRALMIPGMVGKNIEKWLKENNLPKNVAQHGATKEEIFVCYEELKA
                     KYGKEVDNFPLGAIGLYTFSQKIKVGLQQIMAGSRNFRIDTISRSDLMALTEEAAKIS
                     GIPYVMDAYKEEAEKILLEGCDYI"
     gene            complement(22759..24669)
                     /locus_tag="PLV81_05040"
     CDS             complement(22759..24669)
                     /locus_tag="PLV81_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOM09497.1"
                     /translation="MGELKTMVQYFYDTCSKFPERPAQKFNSKLYDDNNGMFTYAELK
                     ERVEDIACALLKIGFLHKGRAAIMAPTSYMWTQVDIAIACAGGVSVTIYPTLSDTEVT
                     YIMKDSKSTVIFVGNKEELDQIKRIWKQLKDLKTVIVMDTSFKGDNKKVYGIKDFIAI
                     GREWRKNAANYKKYIKRKDGVKLDDWFTILYTSGTTGQGKGAIITHWTGSSRLEGTVA
                     YFQKYNFDVNEHDVTLCYLPLSHVFDRGSCQWLALTKGSLICYADKPATLIEDMQKYN
                     PTWINCVPRLYEKIYITMQQAMAESTLKKKLFDWAIGVGLQALEYRKDANGCYNMHPE
                     FDLKSKLPLGLKIKYSIADKLFAKVRGLFGNRFHHSFSASAAISPDLLRFYYALGLAV
                     VEGYGSTESFNAAVLNPITACKPGCMGKEANGSKCRVAADGELELSGAGIFKGYLNKP
                     KETKEAFTKDGWFKTGDVVELTPDGYIKFVERKKGIICTSIGKNVAPAKIENLFSTSS
                     WVEQIFLVGDEKPYISALIVPNFANFIALFKREGIAFDESKLVYENIGGMQICTKVGD
                     DFINNKRLQELIDNEVKEANKRLEKFEEVKRYTILNQRFTESNGMITPTQKAKKRVII
                     QVYKDVIEKMYE"
     gene            complement(24764..27391)
                     /gene="alaS"
                     /locus_tag="PLV81_05045"
     CDS             complement(24764..27391)
                     /gene="alaS"
                     /locus_tag="PLV81_05045"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOM09498.1"
                     /translation="MMKVHDLRTSFITFFKERDHRIVPSSSLIPKDDPTLLFTTAGMV
                     QFKPMFAGTVELEYTRAASIQKCLRTSDLENVGKTKRHCTFFEMLGNFSFGDYFKKEA
                     IQWAWEYSTDVIGFPKEDIWVSIYIDDDEAFDIWHKNIGLPKEKIVRLGKEDNFWGPA
                     GDSGACGPCSELYLDRGPSFGCGKPDCKPGCDCERFLEFWNLVFNQYFQDTEGVLHPL
                     PRTGIDTGMGLERLATLVQNVDSIYQTDELKGLVDYIVKQRNISYKGDAQIAVNVLVE
                     HARALTFAISDGAYPSNEGRGYVLRRILRRALRYGRQIGIHEPFVYTIVDPLVAAMGK
                     QYPELSDTKENVKAVIKGEEERFLETIENGMDRLEEIIKTLTRKGESTISGNDVFVLY
                     DTFGFPVEMTEEIARENGLRIDREGFEYKMEEQRNRGRQSWKGVEGRIDGVVGEILKK
                     VQSTQFEGYEKYDSESEIIVLSDFESLKTHLNEGQEGILVVKNTPFYGESGGQVGDTG
                     YIIGDSGTFRVVDTKKSGDLIVHIGQVEKGTLQEGTKVRLSIDGLRRNLTRANHTATH
                     LLQAALRHVLGQHVKQAGSLVDPQHFRFDFSHFKAMTQEEIQQVEAIVNEKIWAALPV
                     NTAIIPLQEALNQGAMAVFDEKYSDIVRMVSISDFSKELCGGTHVDNTGKIGVFRIMR
                     ESSPGAGVRRIEGVTLKGVYERLIHEHAIVRRIMGLLEVNESNIEKKVAEMLEKVSYL
                     EKELEKAKAAGLKDIARELYTKAEHINSLKFVSASLQNMDAEALRTLADYIRELSDTL
                     VVCLASSKENKAFIIAAATKGAIDKGIDCGAIIKTVSKEIGGGGGGRKDLAQAGGKSP
                     EHIAKALAKCREIVLHSSK"
     gene            complement(27508..28146)
                     /locus_tag="PLV81_05050"
     CDS             complement(27508..28146)
                     /locus_tag="PLV81_05050"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOM09499.1"
                     /translation="MIIQDIEYTDRYIRITLDTGEYFRVDNDSVPSGLLRRGVAIDID
                     AYNHLKEESMLFECKEKALYYISIRSRTEHDIKKYLMKKGFDKTTISRVLQYFTERGL
                     INDYEYAVHFIQSVREHKVIGNKMLERKLFEKGVDKGIIKKAIRKTVDKEPDIEQVYQ
                     LALKKYKQVKEKHNPHVKVVAFLQQRGFGWEIIKKVMKRFGDDYEDIHDMQE"
     gene            complement(28241..28633)
                     /gene="rpsI"
                     /locus_tag="PLV81_05055"
     CDS             complement(28241..28633)
                     /gene="rpsI"
                     /locus_tag="PLV81_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOM09500.1"
                     /translation="MASAVNYATGRRKSAVARVWLKPGNGTIIINNKPINEYFRRQTL
                     ELVVRQPLEEVGVLNSFDVVATVDGGGWTGQAGAVKLGIARCLSALDEKYKKSMRQAG
                     FLTRDSREVERKKYGQKKARKRFQFSKR"
     gene            complement(28678..29124)
                     /gene="rplM"
                     /locus_tag="PLV81_05060"
     CDS             complement(28678..29124)
                     /gene="rplM"
                     /locus_tag="PLV81_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOM09501.1"
                     /translation="MGILQQTFSLKKSQIEKKWYIIDAEGKVLGRLATEIAHVLRGKH
                     KPSYSPHLDMGDNVIVINADKVLLTGKKPQEKEYFFHSKYPGGSRFVNIRKMLEKKPE
                     YVLYHAVKGMMPKNKLARQLMGNLKIYAGSDHPHKAQKPEPLELKF"
     gene            complement(29327..30220)
                     /locus_tag="PLV81_05065"
     CDS             complement(29327..30220)
                     /locus_tag="PLV81_05065"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOM09502.1"
                     /translation="MASNKNDVIPKATIIVNPASRSGLKVYHHLRDCIDKYFNHEVFF
                     TKNRGHATELAHASVSDVIIVCGGDGTINEVANGLAGRTDIVVAPTYGGSGCDLSRVF
                     GIAKRIENRIEEIYQRIINGIDIKIRLSKVITEENFRYFVGVGDAGFGAKVASIFDRY
                     RRLGKIGYVIGVLQTLFTVKPVKVKINIDGEVKIEDVLMIMFARSKYYAGGMKVSPHS
                     DTLSNKARMILLKWMKKIIFLLLFPKVYSGTHVNVRYVEDVDARRIDILTPGLPVDVE
                     GEYVGITPCTFMITDEYIRFV"
     gene            complement(30472..31506)
                     /locus_tag="PLV81_05070"
     CDS             complement(30472..31506)
                     /locus_tag="PLV81_05070"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005495120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOM09503.1"
                     /translation="MKTRITELFGIKYPIVLPGMSWISTPELVAAVCNAGGTGYLATG
                     PLTPEQTRQAIRRIRELTDKPFGAGCTLIMPGARENAEVMLEEKVPIINVSLGKCDWI
                     AERAHKYGGKVIATVVTEKHALAAEKQGADALQVTGHEAAAHGGQVTTFVLVPAIVDK
                     VKIPVVAVGGIADGRGMAAALALGAEGIGMGTRLSMTKESPLHKKTIEAQLKAGIEDT
                     IYSNRFDGIYCRVLKTKSAEKAIRKGMSLPHAALESYKIAKMMNMPYFKLALGVMLQG
                     PRMMIRLAHFATAFDKIKVATEKGDLDYGVQLIGQCQGLIDDVPTVKEVIERTVKEAK
                     AIYQKNMKKF"
     gene            complement(31624..33456)
                     /locus_tag="PLV81_05075"
     CDS             complement(31624..33456)
                     /locus_tag="PLV81_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOM09504.1"
                     /translation="MQKATLEGYKEFYTNEKSVINMVLSRAKKRGNAAALSGFIDGKK
                     YSYTWKDLTKIVYAIAHFLIAKGLQKGDRVAIFSQNCPEWTLADLGIQAAGCVPVPIY
                     ATNSRDEAKYILDDASIKAIFTGDQEQYDKILQIMNSTSLQFVVSVQDAVIVNGENSF
                     YIHEIVKTEVSDNAKKTVDARINELSSNDMLTIIYTSGTTGNPKGAVHTHASFLAGIY
                     ASVFRFPEAGPGMVSLAFLPLSHVFERMWTYGVLLKGGENHYCRNPKDIMEILPLSKP
                     HYMCSVPRLWEKMYATIFDRLETAPPAKKKLFNWATTIGIEYDTRKRAKQFIGPWLGI
                     KRFFANALVLKKVRQIVGGNVWVFHVGGAAMSAEINAFFNGLGIPLAQGYGLTEFFPV
                     AVGTAATAYPGVCGPILDIVDVRVSDEGEIQLKGPMCMQGYLNKPEATREMFTHDGWF
                     KTGDVGTIEEHDGKLYIRITDRIKDLIITAGGKNIAPQVIETMLGEDLYIEQVVVVGD
                     GRKYISALIVPNFEILGEYAKSKGIQYSSRQELISNPAIIEFYNNKIEKLTESLGQVE
                     KIKKFTLMPHEFTQENGEITPTMKIRRKVIQQRYNDIIDKMYLE"
     gene            complement(33511..34587)
                     /locus_tag="PLV81_05080"
     CDS             complement(33511..34587)
                     /locus_tag="PLV81_05080"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOM09505.1"
                     /translation="MYHNKNIHLRRNTMKTKITELFKIKYPIVLSGMSWISTPELVAA
                     VSNAGGLGILATGVLTAEQTREAVRRVRELTNKPFAANVTLYFPGAERNAEVLIEEKV
                     PIINYSLGKGDKIAKAVHAYGGKVIATVTTHKHALAAQRDGADALIVTGYEAAGHGGA
                     ITSLVLIPAISQAVNIPVIAAGGFADGRGLIAALALGAEGISMGTRFMNTKESPVAES
                     AKQASIKSEVYNTVYTPKIDGLPARFMKSKAVERLMKKHLNPLSSLIRSKKIADMLGY
                     PWLKLAVGIMFMGPQRAIQMARMAIGFDAFKIGTMDGDFDKGVLPLGQVTGIINDTPT
                     VKQVVERIIKEAVEIEKQLAKKVK"
     gene            complement(34716..36035)
                     /locus_tag="PLV81_05085"
     CDS             complement(34716..36035)
                     /locus_tag="PLV81_05085"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOM09506.1"
                     /translation="MLEVEKLNLSRIQETCAKQKTRWWDVFAEYAVHKTYSFKNNVFY
                     GVRESEIKGYGIRMNIDGKTGFSFCNDIAMLDSVMEYARETARYGEIEEYDLPAKQPT
                     PHVECYDGNIFNKDTQYYLQTLQNVVDTIRSAYSDCMVDAGLGCVDGYIHLINSRGFD
                     SGYHYSRQGSTISALRILPDGSRVQVYESITWNSDVNIHELSERIIQKLNFSKQTVKM
                     PFGNYYVIFTPKAFGQIMGVVLQGLTGRSIERGISRYIGKFGEKVFGRQFTVYDNPLI
                     DFAPGSYPFDDEGIPAKSKTIIENGYVKTYIAHLQSAHLLHVQPTGNASRGYAILPQE
                     SFSNIVVEPGTTSFKDMIAQMGTGIIIDQFIGFGQSNTLMGDFSANLDLAWLVVQGEV
                     KGRLTNSMVSGDVTTMLNDKIVLSSEREWGGDAYLPYVLCRINYSTT"
     gene            complement(36004..37401)
                     /locus_tag="PLV81_05090"
     CDS             complement(36004..37401)
                     /locus_tag="PLV81_05090"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOM09507.1"
                     /translation="MLDPMDLQKKLKSIKTEYAEIRISESFSTTIHLSKRMIETCKVG
                     TSTQGSVRVYNKGCWGFFAFTSLDMVDYAMQQALQLSNIQKKENAPTLSSLQMNVIKE
                     KTNVLIPPNTVSLEEKMNVLTQYNDILKDSNLIENTNVLYRDSLTQYVLCNTDGSIVE
                     YDRSFCGLSLSAIAKDGMVIQPYGESDANYAGFEIVKDKHPVAEEVVRTATELVQAPQ
                     IKSGVYNVIADPKLAGVFIHEAFGHLSEADFVYENPRMKEIMVIGKRFGPDILNVVDD
                     GTLPYAGYIPCDDEGIKPEKTYLIKNGILHSRLHSRETAVKMGEEVTGNARAITASAS
                     PIVRMTNTYIENGNSTVEELFEKLWNGVYVVGALGGQTNLEMFTFTPAYAYEVKKGKK
                     TKKLRECMLTGNVFVTLANIEAIANDCTLFGGLGGCGKQGQAPLPVSSGGPHILINNV
                     LIGGKGAGSRKTKSK"
     gene            complement(37664..38377)
                     /locus_tag="PLV81_05095"
     CDS             complement(37664..38377)
                     /locus_tag="PLV81_05095"
                     /inference="COORDINATES: protein motif:HMM:NF016873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HOM09508.1"
                     /translation="MKNVSIIITCEHAGNRVPEALRDIFTDTTILSTHWAYDIGALQM
                     ARILAKTLEAPLLYTTVTRLVVDCNRSIGSNELFSELIQGSNIFLKNEIVKRYYTVYR
                     NKIEKAINTMLQSSDAVFHIAAHSFTPQLYGFKRYTDIGLLYDQSRPKEALFCSKIKE
                     ALMQAYPLRVAFNYPFRGCGDGVTVWLRKQYGDSYCGIELEANQQIFFNSKRQWRTVS
                     YHLAQAILYAVNNVVQEDY"
     gene            38467..38823
                     /locus_tag="PLV81_05100"
     CDS             38467..38823
                     /locus_tag="PLV81_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013749653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOM09509.1"
                     /translation="MLIITTIIGLSAGILGGLMGIGGGIIMIPALIYLLHFNQHMAQG
                     TTLAAMIPPIGILAAYEYYKSGNVNIPVAITLALGFVIGGYIGGKFAVSLDNETLRRV
                     FGIILFIMSLHIIFSK"
     gene            complement(38824..40506)
                     /locus_tag="PLV81_05105"
     CDS             complement(38824..40506)
                     /locus_tag="PLV81_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOM09510.1"
                     /translation="MANGAKLVVEALLDKQVDYIFSLSGGHITPIYEHLENSSITIFD
                     TRHEQAAVFMAEAWGRLSRKPGVALVTAGPGFTNALTAIANARLSNAPLLLIAGCVGL
                     ESNEKLDLQDMKQLPVIEPMVKKAFVCHKPERVYEYVDMAYRTACSGRPGPVYLELPV
                     DVLNAAPDETKVKKTNTLVYSKPCDSNAAYDVIELLQHKKKPIIIAGSGAWYADAARE
                     LLQFVESTGIPVFTNAAGRGVIADTHPLCFESSLAIRPGAALYANFNADLVLLLGNRV
                     SLYYIFGDIFNKDATLVQVDIEPEEIGRNRSIYKGILSDVSSFIDECNKIVKERQLNL
                     PEQFKEWVLELKKADEESKKQAQQQWLSNAVPIHAMRVAYEVNQIMDKEDDVVVSDGG
                     DAQIWMGMTRTVKKGGTYLDSGLYGCLGVGIPFANAAKLYYKNSRVVLFTGDGSLGFN
                     FMEIETAIRKGLSITIVVSNDLGWGMIRHSQVLRMGHPVKEGTFIGNIPYHKLVEALG
                     GKGYVVEKPEDIKPAIIDALQSNTVALVNVMTDPDTISPGSVALANLGGYKA"
     gene            complement(40523..41356)
                     /locus_tag="PLV81_05110"
     CDS             complement(40523..41356)
                     /locus_tag="PLV81_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOM09511.1"
                     /translation="MYPDLKNKNAVITGAGKSTGIGFAIARELAKNGANIIIADLGKG
                     TIGDTTMMAGQLSQMQQLAAELQKEYSVTAQALLCDVTNKESINEFAVKVSELFDSVH
                     ILCNNAGATFGVPSAIHTYDDEAWLKTVDVNLHGTFRVSKAIVPLMKQGGSIINTASR
                     AGKFPAMFNGAYSVSKAGVIMLTKVMAKELAGAGIRVNAICPGQINTDLERWRFELEA
                     KVFNTTPEEREKLMCQSIPLNRIGTPEEVARLVVFLASEASSYITGQAINVCGGQLME
                     L"
BASE COUNT        12214 a         9211 c         6849 g        13159 t
ORIGIN      
        1 aattatatat acatttgctg aagtaagaat cataaatact catactcaat atgtataagc
       61 atatctaatt attgtatata tattcaccga aggagggact cacaaacact catattggat
      121 atgcactagc atatccaatt atatatatat acatttgccg aaggagggac tcgaaccctc
      181 atggagttgc ccccggcgga ttttgagtcc gctgcgtcta ccaattccac cacttcggca
      241 aaaaaattag tctattctgg ttgtggtatt tcttccactt caatatattt ggctttagct
      301 cgggcaacta ttttccccat ttcgttttca atttcagccc tattctcaat gacacgctta
      361 ttctttttta caaaccagcc cctgatatac agattttcct caataaaagc tggctgcaaa
      421 taacgaatgg ttatttcccc tgttaaggca atatattcca tgtacttatt tacggtcatc
      481 atagtttcat ctaaaatact agctatgatg ccggcatgga ttatatttgg aggcccttct
      541 aacgtttgat ctgctttaaa ctcaccatat gctgtttttg tgtcgggatc aaacttaagg
      601 tgaagtttta atcctctttc attagatttt ccgcaaccaa agcatgtttt gtcttcaaat
      661 tcaatcatag tggcatattt ttaaaatcaa tgcataattg tcaagaactt tttacgttat
      721 tgagcattta ctcaaaaata gatgatttac cctttttcaa tcatttactt tcattcttta
      781 ttttttccat caataaatca atttcatcct gatcaagcca ctcatctaca tgttccggca
      841 ttgcatctgt agtcatcata tacatttcta aagccataac cagctcttct ttagttaaaa
      901 cccccatggt tacaagaatt tcaccaataa gcctctcagg attatcgcgc tgtagttcca
      961 gagcctcctg caattgctcc tcagaaatga taccattttc aaccaggaaa gctccaacct
     1021 ttttatcatg catatttttc atgtataatt cgcttcgtat tattttcctt aatattaata
     1081 ttaaaaattt aataagtcaa gccaaaaatt atttattaag aactatcgcc catataaaaa
     1141 agtatttaca aacaattcat aacgctatac tttagctatt gttacttttg ctatgaaatc
     1201 aatgagaatc tacatagtgt ggttactgtt accatgtgcc tttttggcag cacgaaaaaa
     1261 cgcttcattt gttggtgttg ctgtctgtaa aaagtgtcat gaatcagagg ctattggtaa
     1321 tcagtataaa tcctggcagc aatcccctca tgcaaaagca tatttactgc ttaaacagga
     1381 aaaagcatta tcgcttgcaa aagaattatc tattgaaaat ccttccgaca atgaaaagtg
     1441 ccttgcatgt cacactacag gcttaggtaa aaaccctgct ctgtgggaag atggtgttgg
     1501 atgtgagtca tgccatggtc caggcagtat gtacaatagt tatgaaaacc atgtggctct
     1561 gggcaacagg gaagttgcat ataaaaaggc cctttcattt gggatgtatc caatcctggg
     1621 aacagatggc ataaaagcca gggaaaaagt atgcagaagc tgccacaatg aaaaacggcc
     1681 atgcttcccc aaagaccctg cagagcaaca gcatcaatac cttcccctac aactgattgc
     1741 tgactttgca tttccccaca ggctccgccg ttaattattc attgtcacta agccagctga
     1801 aacctgctgc ttttgccagt atagtatgca tacgttctgt gttatccctg aataattcta
     1861 ttgtttcagt atgtgttatg gcaggcagtg caaaaatttc cataccagtt tcaagataca
     1921 gctgacgcaa gctaccttta taagctgttg tactgcgttg catgtctgaa gtttcatata
     1981 atgaatacag cagcgaatgt aattgttgtc gaaacataag ctgcaccagc agagcataca
     2041 cctcttccag agtatagata actttggcta tacggtgtaa tttaatgatg tcatgaatga
     2101 aaatatcact ttcttcactc ttgtacaata ccagtgcatt tttctttgga agctttccgg
     2161 ctatatactt tgtagcaatt tcatgagtgc taacctgtga acaattaatg cacaccatgt
     2221 ccatgacatt atgaatatca cagcatgctt caagtgaatc cactactata gcctgatagc
     2281 caaatgcctg tataactgtt gataagccat acgtattgaa tacattatta tcaattaggg
     2341 cgatagttcc tgtaattgca ggagtgtact gtttaaaaaa taatggaata tagtgatact
     2401 gtgatggcct gaacaccgct gttattccat agcgtataag tgtttttttt aaagattgaa
     2461 tttcagaaca gatacctata cagggaagat tggcagtaac aatttcttta ataagcgatt
     2521 catactgcac agccatacca ctgctgttaa aatcaataat acaggcaata tcgcgatttt
     2581 gttttaatac attgacactg aagtcttctg atgtgcattc tgctgtaaca gaatgttttg
     2641 caagctcact ctgttgtgcg gtattattgc ttatcagtac aaatgagtgc attatactta
     2701 tactatctcc agcgcttcta cacggtgttt cagaatatca ctcatggaaa gcactgtatc
     2761 agcactttcg gttatcttca atgctccgga tgcatttgcc tgagttagtt cattaatgcc
     2821 agcaactgat ttcacaattt cacctgatgc atttttttgt tcttcggcag cattacgtat
     2881 ttcctcagac ctcaccttga cctggcctga ctctttggtt actattacat ttacctccaa
     2941 ttgctgcttc atataatcag ccagcacctt taccatggca tttatttcat ttaccccttt
     3001 gataatacca cttatagtat ctactgtctg ctgcacactt gtcatacctt tctgtatttc
     3061 tttgtcatta gcactaatta atgactcaat atcctttaca ctgactgatg tcctgtcagc
     3121 taactttgat atttcatctg ccactacggc aaaacccctg cctgcatcgc ccgcacgtgc
     3181 tgcttcaatg gcagcattca aagacagaag atttatctga tctgagatat ccttgattat
     3241 cttcactata tttgtcatct cacctgaact ggtatgtatg ttagtgatac ttgtatccat
     3301 gccgttcagt aattcagcac ccaaacgagc atgagtttct attgaagcgg tcagctgcag
     3361 tgcattgttg atttcctgtt ccatccgtga aacaagtgac gatagttcct ctgtacgccg
     3421 tgtaaatgaa ttgatgctgt catactgata atttgcactg tttgacacat tttcaacacc
     3481 agccgaaatt tcttcaatgg ttgccgttat ctcttcagct aaagctgcct ggttttgtgc
     3541 attaactgaa aaatttttgg aagtgtccga tagctcatgg gaagcaactg ccagcttgag
     3601 cgaaatatcc tttaccgctt ccagcaggtc tttgatacgc atcaattgat ccctgttttc
     3661 aagctcttcc tgtaattttt ggattgttgt atccatgagt cttaacagca gatagcttat
     3721 agcagtaatt attatcaatt caaaagtaaa atcaacaata gcaaccgttt ttaccttacc
     3781 tgaaataagg tcggtacgta taaatgaaat aatcgcaaat acaagcgaca caatagccac
     3841 tgttataacc atgcggcgtg aacaaaacaa tgcgctaaag ataataaaaa ttaaaaactg
     3901 atagttggta agaatgatcc cttcattatg tgccattgtg ccaaaattta ccagaagccc
     3961 taatacaaat actgaaccaa tcaccgaaat atttgctgcc catgaataat tacctgcccg
     4021 caatagcaga gtagcaatgg ttaaaaaaat aagtatgtca cataaaactg cattaataaa
     4081 tgaaaatagt ggacgtgttg ccattagatt ttgtgcaaca agaaaaaata ataccagaac
     4141 aatgcatact gttagaaaat aagaaagagt tttagcacgc tgctgcatta taaacgaagc
     4201 atcgctatat tttgcaagta cattcctcat tacatccata gatactatct cctgaagtag
     4261 tttgttgatt tttatgccat gtgtaagttt ccgcactgca atactatatc tcgttaaaac
     4321 aaatgcaatt ctatttattc tatactttag attttatcaa gtaaaatata gtatgcttac
     4381 cccaggcacc tgcggcatga gtatcttcac ttatatatta tttaaagcaa tgattcttta
     4441 attaaaaaac tattgctaaa taattttatg tttgacttat atttttacat attgtatttt
     4501 ttagttgttt acatataact gatatgtcaa ctccccatga aaaattttta attttattca
     4561 aaaaaggagg aaagtatggc tggtagcttc gatttttcac aatttattaa tgattcaaaa
     4621 gcaacagtat taagtcccaa ggattatttt tcatcaatgg ctaaagaagg tggccttgtc
     4681 gaacccatta tcaaagcagt tttgtacgga gtaatttcag gcgttattta ctttattttt
     4741 acgttactta acctttctgc ctataccatg tttggcacag gtgctgccat ggctttaatt
     4801 ggacctgcca tcttcgcaat tattgggctt tttattggtg gcgtcattat gctcattatt
     4861 tcagcaatat gcggcggcaa tactgccttt gaagcaaatg cccgtgttgt ggctgcatta
     4921 atggttcttg gtcctgttca ggcattattt tcatttttaa caagcatcag tttttatttg
     4981 gggcttatag tatcagcgtt aattgcactg tatggcctgt accttacctt tattgcactt
     5041 gtgaacgcgt taaatgctaa agaaaatgtt gcaaagatag tcataggcat tcttgctgta
     5101 ttgtatgttc tttcactcta tggttcattt aaagcataca gatttgcagg acacatgggt
     5161 gaacagatgt tagaacagac cgaaaagctc tcttcagaac aacagaaggc tttagaacag
     5221 ttacagcaat tacaaaagtc attagaaaag cttgaacagg aacagaatga ataatcttac
     5281 aaaagacccc ctcttaaaaa gagggggtct tagtatttat atctttttta ccttataccc
     5341 tgcagcaagc gctacgttta catgaatcac gccatcattc aaactcttaa aaggatagct
     5401 atcatcaata acgggaagat tatcagcatc gtgttgagtt ttaataactg accctgcagg
     5461 tacatatctg ccatcaggta tggtcactcc cataaccagc gattttggtt ctaccacaca
     5521 gcctttgcct actcgtgccc tgaatacaag actttgcatt cctataaagg aactatcgcc
     5581 cacaaaagcc ggaccgtgaa tctgtacctg atgagcaagt gacacacgag taccaacata
     5641 tacggcatat tttttaccac ttacttccac cagatttttt tctaccggat gaccatggtg
     5701 ttctgtttca agggcatgaa tgataacacc atcctggaca tttgaatcat cgccaacata
     5761 aatgggctgc ccttcatccc cccgcacaga agcaaacggt gctaccataa cattttcccc
     5821 aagtatcaca ttcccaataa ccgcagcaag aggatgcaca taggctgatg catcgatgac
     5881 cggtgtgtat tccctgctta caaaatcggt ttttacattt tcatgaatca tagatcctcc
     5941 aaaattgcat ttttaattat cccttatgta ccattacaat ctgtcaacat aatttatatt
     6001 gtttttttaa tgaaagaaaa aagcgttgat gaatatgcat ataattattt aattatactc
     6061 tgtatacagg atatggtatg tttaaacaat ttttacataa gcatggcatc agcaatataa
     6121 cgctgcttgc cactgaatcg ccgttgcatc agcggatcgt gagctatctt tcacgcaatc
     6181 atataacata tactgtaatt gccactgatg accctttgcc tgatacacat tcccttttaa
     6241 tactaccctt gagcatgcat accattacac cagtaattga tatcttacaa aaaatgctta
     6301 catctacata taccccgctt ttactgtttg ctaccgattt ccccataact acccgattta
     6361 aaaaacgttt tagtattgca ggaattatcg gcatacttga actgacggaa cgccctcact
     6421 tcatcacgtt agaaactcag tcactgcagt caaatcccac agcttcactg aatgcgctaa
     6481 tctatacaac tgaaaatctg atttcacgca atatatataa attgctatcg caaaacattt
     6541 tatataatgc tctggttaaa ctgcatccat cggtagtatg catcaccaca ttacagggaa
     6601 caataatata ttacagagct caccgacaat attttacccg aaacaataat attaaaaaat
     6661 tattgtacgg gaaaaaaatt caggaattaa ccacacaacc ggaaaagata gaacatctgt
     6721 tttcattaat tgaccgcaat attgaacagc gtgaatcaga aataactttc agaatgaata
     6781 atactgacac cacactgctt gtgacgggcg aaaaaattaa gctgtatggt gatctggaat
     6841 tttacctgct tattggtata aatatcacca ctcaacgtaa agctgaggac atgctcattt
     6901 cgtctgagct aaggtatcgt acgctggttg aaaatcttcc tgatgggctg tcactttcaa
     6961 aaggggatat gcttttatat gctaacccac aattttttaa attattcaat ttcaccacgg
     7021 aaactctgat tacggatgtt aaccttaaat cactcatcca tcccgatgac tgggcagcac
     7081 tttatgaact cctacaaaaa cccattggca atgtagcaac tataaatgtc aaagcctata
     7141 ccgctaccgg taaaccgctg atacttgagc ttagtgtctt aagttttatg ggcgatagtt
     7201 ccttatatca ccagatttac tggcacgata taacgcaaac acgccagctg tgggataaga
     7261 tactgcagtc tgaacgattg tctgctatag gggaaatggc ttcaggtatt acccacgaat
     7321 tcaacaatat tctcacaagc attcagggct acgtccagta cactctgaat aatcctgatg
     7381 atattgaagc caatacaaag gcctttaagg ttattgaaaa gatgaccgaa cagggccatg
     7441 ccatccttaa aaatctttcc atgcttactc gtaaggacat tactaataaa gaacgccata
     7501 acatagcgga tctcattaat gatattgtac gtatacagga aaaaatatta ataaaggaca
     7561 acatcactct gataaaaaaa tttgtaaaaa atccagtgtg ttatgtggat gccggcctta
     7621 tacagcaagt atttttaaac atactgctta atgcagtaca tgctatacgg ccaaaaggtc
     7681 atggaactat caccattact gttgatgaat caaatggcaa tgcaattatt aaaatccatg
     7741 ataccggtac cggaattgac ccaaaaattc aggatgaaat ttttaatcct ttttttacaa
     7801 ccaaaactgg aacacataac atcgttggca caggaatggg tttatccatt tccaaaaata
     7861 tcattgaaca gcataacggg aggatctggt ttcatagcat ccctgacaaa gaaactgaat
     7921 ttattataga acttcctata tataaaaccg catatctacc aaaggagttg gaggcttctt
     7981 cgcaaccaaa agatttaagc aaccttaccg tgttaatagt cgatgacgac gatgcaatcc
     8041 gtgatctttt taaaacactt ctacaatcat taggcatatc cgctattaca tttgcaacta
     8101 atggctatga aggttactcc ctgtgcaaag aaaatcagta tgatattgta tttatggatg
     8161 tatccatgcc tgtactttct ggcatagatg catacaaaat gataaaagat gaaaatccca
     8221 gtcagaaggt tgtatttatc accggtattt tttatgaaga ccagattaaa gagttagttg
     8281 acaaagaaca ggcatttggc tacattaaaa agccatttga cataaaagaa attaaatcat
     8341 tgttatttac aatagcatca catcaataat acctggagaa tgaaattgat tatagatact
     8401 tttttcaagg gagaaaacct atgaaaccaa aaattgatgt tggcataatt ggaggctcgg
     8461 gcctgtatca gattgaaggg gtcactattc tggaagaagt aaaggtaaga acaccctggg
     8521 gcatgccttc agatgctatt accattgcag aagttggcgg agttaaagct ggatttttac
     8581 ctcgccatgg ccgtggacat tttatcctgc ctcatgagat taactatcga gcaaacattg
     8641 catcactcaa aatgctgggt gcaggccaga ttgtggcatt ttctgcagta ggcagcttaa
     8701 aagaacgcat caagccactt gattttgtaa tacctaacca gattattgat cgtacaaaat
     8761 cacgcatttc aacctttttt ggtgatggaa ttgcagcaca tattgccttt gctgacccat
     8821 tctgcactag acttcacgaa ttaatacttg ttgcagcaca aaaactgaac atacccatgc
     8881 atactaatga aacccttgtt tgcatggaag gaccagcatt ttctacccgt gctgaaagcc
     8941 acctctaccg ctcatggcag gcaggagtga tcaacatgag tacattacct gaagccaagc
     9001 ttgcccgcga agctgagatg tgttatgcag taatctgcat gagcactgat tatgattgct
     9061 ggaaggaaga tgaggagcat gtaactgttg atatggttgt taataatctt aataaaaatg
     9121 cttcgaatgc caaagcactt ataaaagaat taataccatt gcttactaaa gaaagggatt
     9181 gtggatgcaa ggaagcaatt aaatatgcaa ttatcaccga tcccaaaaag caaagcagca
     9241 aacaaaagaa aaaactaaaa gcaatattac ctaattactt ttcttaaaca atatgatgct
     9301 gggtgctgtc atggcaagtg gctgtggtat catttgccat ggcagcatac aattcgtgtg
     9361 ccatttgcag aagtgtttcc tgattattca attcagggtt gtcaagtact tgttccagta
     9421 actcattaag aattttgcct attattggcc cgggtttcaa tttaagatgt tccatcaaaa
     9481 tatatccatc tatatctaag tccttaacag taaaggcatt atccttttct attactcttt
     9541 gaatatgtcg tttaagcttt ttgataggtt caggcaatcc atctctcatt ccattgccac
     9601 atctgtcagc aaggcgcaat aggaatagat catcaagatt atctaaccca acctttcgta
     9661 taaaccggcg gactgctcca tctgtccagt catttgtata atgaaacatg tggtttgcaa
     9721 taagattggt gacatactct atttcatcat tggaaaattt aagcctgcgc atgatcttgc
     9781 gtgcaatttt ggaacttatc acctcatgat tgtaaaaggt ataatcacca tcactgcctt
     9841 cacgccgtgt agggatttta ccaatatcat gcagcaatgc tgcaaggcgg accaatggtt
     9901 tttcctttgg tgcagcatca caggaataga tgctgtgata ataaatatcg tacatgtggt
     9961 atttattctg ttccattcca aacccttgcg atagctcagg catgtaaaga tctagtaatc
    10021 ctgtaacacg catatactca aatccctcag atggtttatc cgcttcaagc attttcttta
    10081 tttcatcgcg tacccgctct acagatatct tttttgctat ctcgagtact tgtggaattg
    10141 cttccagtgt tttcttatga atgctgaaat gtagttttgc agctaaccgg catgctctga
    10201 atggtcgtaa tccatcctca ctgaaacgct gaaggggatc ccctatagtg cgaattatct
    10261 ttttttcaat atcgtccatg cctcccacat aatcaatgat tttgctggtg ttgatatcat
    10321 aggcaaggcc atttatggta aaatcacggc gttcaacgtc ttccttaaga gtttttgaaa
    10381 aaacaactgt atcaggatgc ctgccatcag tgtatttacc atcagccctg tatgtagtta
    10441 tctcatactg cgcatagtgc ataagaactg tcaccgtgcc atgtttgata cctgttggca
    10501 caactttttt aaacagcttc attacatctt ctggatgcgc attggttgca aaatcataat
    10561 catacacagg tatccccaat ataaggtcac gcacagaacc accaattaaa taacactcat
    10621 gcccattgct ggttaatgtt gtacatatgg ccttaatttc tttagctact cttggaggaa
    10681 ttgctaatgt tacggtatac gtcttcataa aatgtgctgc tttactccag tatcctgtaa
    10741 ttgccgccat gttttaagca acagggccat ggcatactca gcatgcgacc acatgagcgg
    10801 gattgcaaag gtaagatagc gtccagatcc cgtttcaatt cttcttttta tctgatcata
    10861 agaatttttc atatgattga gttcctcaac aatcaaatcg taggttattc cgtcaaccat
    10921 aatatcaggt gcaaattcct tgttcacatc acttccaggc aaccactcca atgtcataaa
    10981 ttcatgaaat ctatcaggcg tggtcaaatg ctcacacaaa tagccatcag tgctatagct
    11041 atctataata tccataattg tatcaccttt ttcaacgttc cctgtaaaat aataatactg
    11101 tgccagcatt gctgtatact gcacccaggg accattacca gcatgtacat gggtattggg
    11161 atcctcaata aacggcagat agcgctgtag cattcctaaa tcagggtccc atagggtatc
    11221 ctcgagaaat ttcattgtgt taataaaaat ctcatctgcc ttaaaaatat ccggcactat
    11281 ccctgtacca aagaaaaacg ggctcattaa tgttatatca gggcgcaaat cggcctcacc
    11341 atcaggttta agccttcgta taagcctgtc cccaatggta aaaacgtttt taacaatggt
    11401 gccaaatccc tctttcagta caaaagcttt ttcaaattta gtttcacagt taaattcttt
    11461 tgccacctgc tcaattaaaa gcaacatgca gtaatatgca taattaaccc atattgaata
    11521 accttcttct attgctgatt catggatgga tgttgtggaa taaaataaat gtatttcatt
    11581 acgatagtac cgtttaaccg caaacattgc ccctttataa attgcctcaa tatatgcttc
    11641 attatcatga ggaatatgac ccctgttttt ggctgcaagc agatatctgc acaaaatgga
    11701 tacaccatgc gccacattgt cttcctgctt gtaaatgctt ttgtcagccg catcaagccc
    11761 ataccgctga ccccagtatc catcgtcacg ctggcaattg taaataaatt tggcaatacc
    11821 ggcaagaagt ctaaatgcaa tatcttttga acgcaacctg ctcattgcaa tacggtacag
    11881 cattcgagtt gcacaggcac tatcgcgcgg atacacatag ggataccgtg aaccaggtaa
    11941 cgttgcaagc agggcactcc cttttggtgt ttcaaacgta cattgtgcaa taacatcaat
    12001 atgcttattt attgccctat ttaattctat aagctttttt gatataaatg cttcattgct
    12061 gtcaaaaagt tcatgccctg tcacccgctc aagtgcaaca gcctgaatag tattatagac
    12121 atcaacatga cgcgctataa tggaatcaaa atcattttta tcaatcttca gcaattcaca
    12181 gtcatcgtta gctataacag tagcattccg aggttcaccc agtaccaggg ccatctcacc
    12241 aaaaaaatta ccctcagcaa gtaacgatat gagttcctca tttttattca ttgctctgta
    12301 aacactcaca tccccttttc ttattaaaaa caatgcatcc ccaacatcac cctgtttaaa
    12361 aatcacatct ccttttttta cgtgtaccag cctgacaaca tccagtattt cattgaaaac
    12421 ctcttcgcgc agatgtgtaa aaattggtat ggaccgcaga ttgttacgca ttttccgctg
    12481 caaaaaggca ttattgagtt tttcataagt tgcagccgaa gatgcaataa tttttgttaa
    12541 ttcctgcttc ccaattgcaa gtaaaatact atcagtaaat gctatcgccg tactttcccg
    12601 tggctcattt cggattacaa tgtcctcacc aaaaaagtct tcaggcccca atgcatataa
    12661 ctccagtttg gtataatcag gcgtaataat aaatactgat atttcacccg aaagtacaac
    12721 ataaagccac tcattgtatc ttccatgcct gctgataatt tcatcttttg catactgatg
    12781 aatgaatatg ccatcaacta tctcctgtaa aaccgcttca gggacatctt taaagaatgt
    12841 taaagacttt accgcctcaa caggcgatac cttttgcaaa agagggaatt gcttcattgt
    12901 cccaataagt tcactgatag taaatatctt catataatta cctttcatta atttttgatc
    12961 aattttattt aataatagag tattataatg acaattgcaa gtataattga agtttcgttc
    13021 tacttaatat ctgatcctgg gtcagggcgg gcacttactt cttttgaatg ttttgattca
    13081 tgattgaaca tggtatcagg cttatatgaa tcataggcag tcactgcaaa aaaatacaga
    13141 acattgttct ttaataccgg aaacgttaaa aacccttttg tatcattggc acggttttct
    13201 tcaatgacat catttgttag tgttactgta atatatccat ttgtgtccga cagttcatta
    13261 gaaatgcgtt tgccattaac agttcgtata atgccatcat attgtttgct tttaacgcca
    13321 tagtaaatgt tataccccag tatatcgtac tctacgttct tcttccactt cagcgcgacc
    13381 atattgttac caaccgaatc aacagtgaca aaaatgggtg gttgcggggc tgtatctaat
    13441 gcatactgca acccaatatt gaggataacc ggcgcatttg atccatcggg tgaagctatt
    13501 aaatgtgccc gccactgata gaatttgcct ctaagatatt cattgccaac cctgtgcaga
    13561 tatatatttt tttgcttatt tgttattcta taccattgtg gtgtttcatc atttgccttg
    13621 aagaacgcat cactgattct aaattccatc catatatatg tggatggggg catcttttca
    13681 tcccaggcaa aaagcgttac cattgtccca taatccggga acgtatatac agggcttgtt
    13741 atgataccct cccggtttat agtatccctc tgattattgg ttatagcctt aaattgtgtt
    13801 tttgctattt cagtctcttt ttctaaatcc agaatatatc gctgggcaat ccttactttg
    13861 tctaaatatc cataaaaatg tttgccaact acagcaaccg gtatgtcctg acatacaaac
    13921 cgtggcacca taacatcaac aaacggctca ccactttcag tacaaaaaat agtttctaca
    13981 acatccccat ttatcatagt ttctaatttt cccgatattc tgtcaaaact aaccgcaata
    14041 tggtaccagg tatttaatgc aagtgacggt gaacggtgac agaaaacatc aaccggaaca
    14101 ttatcctttt tataaaacat tttgtaaaat ctgatggcaa gcttatcatt aacacacaca
    14161 atctcaaaac cctgtttacc gcctgaatac ccaattcgcg aaagaactac tgccagggga
    14221 ctgtatttgg taagcttcag ttttgcttca atggtaaatg atcctaaatc ggtacagttt
    14281 gttaaccagt tattctttgc agtttgtatt tcaatacgtt ggtctttgtg aaaaaaatat
    14341 gcacatcctt cgttttcaat atcttctttt gtatatgtat actgtgcgta tcgtattgca
    14401 taatggccag aatcatcctt gaccatggca gggaaatcct tatcaaaggt aatcaataaa
    14461 tcagtaacca gtggattgac ggattcttta tagtttattt ttgcgatagt aaatcctgat
    14521 tggttcccct gcaatttaat tttttttaca ttaaacaggt ccaacccatt agaagtaaac
    14581 atgatctggt atgtggtatc atacgtcagc gcaatacaca acaccgacag aataataata
    14641 cctgtaatag cataatattt ttttatattg acctttttca ttccattatt tctatttgtg
    14701 caattttatc taacctgcgc tgatggcggc caccttcaaa tgtgccttgt aaaaatgctg
    14761 ctgttatgtg aatagctaaa tccattccaa caacccgcgc gcccagcacc agaatatttg
    14821 catcattgtg ccttcgtgcc atggtagcca taaactcatt ggtacacagg gcagcacgaa
    14881 tgcctttata cttattggca acaatggatg cacctattcc tgtaccacat agtgctatgc
    14941 cataatccac ttcccctttt tgcaccagtt cagcaactaa tttaatataa tcaggataat
    15001 cgcatgattg tgtgacatca gtgccaacat taataaaatt atgctcttta aaatgctgca
    15061 taatatattg ttttaactca acgcctgcat gatcattgcc aattgctatt ttcatataca
    15121 attcacctgc taaaatcgtg cgtttcccgg cgtacgggga aatggtataa catcacgaat
    15181 attctgcata ccggtaataa actgtattgc gcgctcaaac cccaggccaa aaccagcatg
    15241 tggtgctgaa ccaaatttcc gtatatccat ataccaccag taatcatcag ggttcaatcc
    15301 catttctttc atacgtgtta tcaacacatc gtatctgtcc tcgcgctcac ttccaccaat
    15361 aatctcgcca acacccggca ccagtacatc cattgctctt acagtgttat tatcgtcatt
    15421 aagcttcata taaaaagctt ttattgtctt tgggtaatgt attactatta ttggcttttt
    15481 gaaatatttt tcagttaacc agcgttcgtg ttcagtttgc aggtccatcc cccagtgtac
    15541 cagaaattca aatgactcat tagattgctg taatttttca atggcttctg tgtatgtaac
    15601 tatctcaaac gaactgttaa caattaattc tagattctgg ataatgcccg gctttatccg
    15661 ttcattaaaa aactgcatat cctcctggca tttctcaagc acccatttaa aaataaaacg
    15721 caaaaattct tctgccaatg aacaatcatc ttcaagggtt gcaaatgtca tctcaggctc
    15781 aaccatccag aactcggaca aatggcgcgt ggtattggaa ttctcggctc tgaatgtagg
    15841 cccaaatgta tagatatcac caagcgccat ggccagcaac tccccttcaa gctgtccact
    15901 aaccgttaaa gctgctttag caccaaaaaa gtccttggta taatcaattg caccattgac
    15961 catcggcaaa ttatgtaaat caagtgtcgt cacctgaaac atctcaccag cgccttcaca
    16021 atcgctggtg gttataatgg gtgtatgcac atacaaaaat cccttttgct ggaagaatgt
    16081 atgaatggca tacgatacct gattgcggat tcgcatgatg gctcccatgg tgtttgttcg
    16141 cgggcgcagg tgtgctatct cacgtaaaaa ttcaaatgaa tgcctctttt tctgcaaggg
    16201 atagctttca gggtcagcat cgccaaacac agtaatgata ttgggtttta gttcccatgt
    16261 ctggcctaca gcaggggatg gcaccagact gccctgtata tacacacttg cacccgtatg
    16321 cagcctttgg gctatatcaa aggaactatc gcccacaccc accaccacct gcaggtttga
    16381 caggcaggag ccatcattaa tttcaataaa gatattttcc ttggattccc gttttgtgcg
    16441 cacccatcca gctactatgc aatcatcaat aggaatatca ctcttcaaac aatctactat
    16501 tcgcattcgt ttcatatttt tctttggtgc ctttcacgta cccatcaatt atttcatcta
    16561 taaaaattat tggccttcct tgcgtttctt taatgctaag gcttccttta ttcctttttc
    16621 acgtatcacc ttaatcttta aaccaaactc agtcattcgg aagtaccgct ttccatattt
    16681 tgatagtaat tttttgtcaa tctcaaaacc aagtcccggg cgtgtaaatg gctgcagcat
    16741 tgcattttta tccggaataa ttggctcaat gatgccatcc ctgaattcag gaatccatga
    16801 aggttcttct agtggatatt ccagtggcag tttattttta gtatcagaaa gtaccatatt
    16861 ccagttaata taaaatccta tgccattggt ccatgtatgc ggtgaaaact ctcgcttttt
    16921 ttcatggcag gtatcaatga cctttttgac ctgcgcaatc cctccggcaa acgttgcatc
    16981 gggctgatat atatggaaac agtccttttc aaacattatc tttatttcat cccatccgta
    17041 gttgagttct gcgcctgaaa tcttaacctt ttttgcaaat cgctttaatg ccgcatttcc
    17101 atcataatca cgtgaatcca gcggttcctc aagccataca atgttgttgg cttcacatgc
    17161 tcgcacaaac tcttttgcac gctttaaatc ccagtcagga accctgtcaa ctattgaaac
    17221 aggccatccc tgattggcat ccactcccag ttttactttg ttaccaacac cttttgatat
    17281 aatttcaata tggcgtatgt catccttaag ttctttattt tttacccgca gtttaaatgt
    17341 tttaaatccg cgctcagtta actttaagat ttcatcaaca cgcttgtgtg gatcatgcat
    17401 ttcaccggtt gaacagtata cctcaatagg gcgtgcattg ccacccaaca gttcatagac
    17461 tggctttcct ttactcttgg caataatgtc ccagaatgcc ggctctatcc agaaatttct
    17521 ccaccccaaa taccctgcct gttttaaaag gctttgaaca cggtcaatat catttggatc
    17581 agttcccatg atatacccgg ctaaaagatc tccaagccct tcacgctctt tacccatagc
    17641 cgtccctgct gaataccctt caatgccatc atccgttata agctttatca acgtaaatcg
    17701 gttatgtgtc tgtggatacc cgggaatcca cgttggccag aatgtatggc gcaatggtat
    17761 agaaacatgg tataattcaa tacagcttat tttcatggct aacccccatt cgtatagtat
    17821 gttaaatacg ttaacagata gtaatgtccc gtcaatcaca attcaattgc tttacaatat
    17881 tctcctacta taattgtaaa attacaaact tttatgttga caggcatggt ttgataattg
    17941 ttttatagtt ataatttatt tgtaccgata gttactgtta atacatcaac atatctttgt
    18001 gtatatcaca ataaaatttt cgtaaaagat tatgcagtga acgttgagtg cgtagaaaaa
    18061 atattttcag ttgatagaac atgattactt atcccgatta attaaaatga aaaacaacaa
    18121 aaacaaagga taccaaaact attttcgagg aaaacccatg agatttattt ttgctgcctt
    18181 attattcttc ttatcagttt ctccactcta tgccagcaat aaagtttatg tacttgttta
    18241 tcataccttt atgggaaaaa aaattaaata tgacatatca attcaggaat ttcaaaaaca
    18301 actgcttgat ttgcgttcgc atggattttc ttttatatca tttgatgata tcaaatacag
    18361 aaaagttagc ggtgataaaa atatccttat caccatcgat gatggccacc acacagttct
    18421 tgatgcatat tattcagtat taaagccttt aggnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18541 nnnnnnnnnn nnncgttttg tagtatccat ccccggtgta gtttttggta ttaagggatt
    18601 tgggccatta ccaatggcaa taattgccac atcaatatca ataataaact ctgacccttt
    18661 aacaggtatg gggcttcgcc ttccggaagc atcaggttca cctaattcca tctgaataca
    18721 ttcaaccgct ttaaccctgc ccttttcatc accaataaac cgcacaggat tggtcaaaag
    18781 cttaaagata acgccttctt cttcggcgcg ctccacttcc tcaattcgag caggcatctc
    18841 ttttttggaa cgacggtaaa taatatatac ttctttacta cccaaacgca gtgctgtacg
    18901 tgctgagtcc atagcaacgt tgccaccacc aacaacagcc accttttcac ctttaacaat
    18961 tggcgtatca tactcaggga agcgatatgc tttcatcaaa ttggaccgtg ttaaatattc
    19021 attggcactg tacacaccct gaagattttc gcccggtata tccagaaata atggcaatcc
    19081 tgcgccagtt gccacaaaca ccgcatcaaa accggaagat agcaagtcat tgacagtttc
    19141 tgcagtgcct atcaccctgc tcagttctat ggtaacaccc attgctttaa gagcatctac
    19201 ctcatactgt actatatctt ttggcagcct gaactcaggt attccatata ccagaactcc
    19261 accagcttta tgcaatgcct caaacacggt aacactgtac cccatacggg ccaaatctcc
    19321 cgcagccgtt aaccccgctg gccccgaacc aatgatggca accctttttc cctttactgt
    19381 ttcacctttc gtgaatgtat aatgctgttt ttcgcgttca taatcggcaa cgtaccgttc
    19441 caggttaccg atagcaaccg gatctccttt ctttgccaga acgcacaact tttcacattg
    19501 atcctcctgg gggcaaaccc tgccacacac tgccggcagc gaatttgtct gctttataac
    19561 agcaaatgcc tccagtggat tgccctcttt caccgctttg ataaatcccg gaatattaat
    19621 attcacagga cacccttcca cacaggtagg gcgcttacac tgcaggcaac gggttgcttc
    19681 aagcactgct gttttttcat cataccccaa agggacttcg ttaaagttat ggatccgttt
    19741 ggctggatcc tgctgaggca tcttttgtct tgggatttta tcggccatgg tatttctcca
    19801 gctgacatgt atgtttttca aagctttctt tttcctgatc cttataggtg ttgagccgtt
    19861 tcatgagttc atcaaaatca actaaatgag catcaaactc aggtccatca acacaggtaa
    19921 attgcttttt gccatcaact gttactctgc acccaccaca catgccggtg ccatcaacca
    19981 taatagaatt caggctcacc agcgttttta cattatacct gcgtgtgagt tcactgaccg
    20041 ctttcatcat gggtacaggc cccactgcaa ccacaatatc aactttctta gaatcaagaa
    20101 tctttttcaa ttcatcagtt acaaaacctt taatgccata tgacccatca tcggtagtaa
    20161 caataatctg gtcacttttt gctttcagtt cctgctccag tatcaataaa tctttattcc
    20221 gtgcaccaat aatgtttata caaaaatttc ccgcttcctt cattgcaact gctatagggt
    20281 acacggcagc aataccaatg ccaccaccca cacacaccgc tgttccatag ttacgtatgt
    20341 ccgtggggtg tcccagaggt cctgcaacat catatacata ttcaccttct tttttgagcg
    20401 atagtttatg ggtagaggtc cccacaatct gataatacag ggtaatggta cctttttccc
    20461 tgtcagaatc agcaatagtg agagggattc gttcgcctac ctcatcaact attacgatga
    20521 caaattggcc tgccttacgg tggtgtgcaa tatacggggc ttcaattata gccttgccca
    20581 cattttccga tattttacta ttttgtatta ttttattcac atgtacctca ttttataaac
    20641 atgaaatttt attatatatt aaaatactta atattttaca gtttttcaac atatattttt
    20701 tatcttgcag aatagcctca ttgattacaa ttatttacta atacaggaat gcaagaaaaa
    20761 attattcttt tcaaatttat cttcttatgt tacatatatg ttaaatttta aggaatttat
    20821 atttcataca cattaaatat tttgactttt aaaccaatca accttcaata acacaaaagt
    20881 tcttaaaaat atctttttgc aaacttgtta ctttaaaaat ttttttgatc agcactgtca
    20941 agcaaaagct taaaaactat aacaatttac aaaggagcac acttatggga aatctatcac
    21001 gaccaaattc taacgatgca acccgtaccg ctaaccgttc aaggagtgta gcaccacagt
    21061 ctggtatttg ctcccggtgt ctggatggct gtacagggaa ttgcgaaatt ttccaggcca
    21121 ccatgcgagg acgcgaattg ctatatccgg gtccttttgg tgagataaca gcaggggctg
    21181 ataaggatta ccctgttgac tattcacacc tcaacattca ggggtatgca gctggtgcaa
    21241 agggtctact caatcctggc cctgaaactg cacggtttcc attagtttca acagaaacgt
    21301 attttggaac taaaaacagg gtcaaactgg aagtaccaat gtttaccggc gcactgggtt
    21361 caaccgacat agcacgaata aactgggaac attttgcagt tggtgcagcc atttcaggta
    21421 ttgctattgt atgtggtgaa aacgtatgtg gaatagaccc cggtctggaa agggacaaaa
    21481 atggcaaggt tactaaatca cctgaaatga tccgtcgtgt agaaaccttt aaaaaatata
    21541 aaggtgaagc cggggatatc cttgttcaga tgaatgttga agataccaac ttaggtgtgg
    21601 ctgaatatat tatctcacag cttggtgttg aaaccgttga attaaaatgg ggacagggtg
    21661 ccaaatgtat aggtggtgaa ataaaagtta gaagtttaga aagagctata gaacttaaaa
    21721 atcgtggata catagtaaca cctgacccaa ctatccctgc tattcaagct gctttcaaag
    21781 atggtgctat taaagagttt gagcgccatt cacgcttggg attcatcagt aaagaagctt
    21841 tccttcagga agtagaaaga ctcagagcat tgggagcaaa acgcgttaca ttgaaaacag
    21901 gtgcgtactc tcaaaaagaa cttgcagctg cattacgctg ggcatctgaa gcaaaaattg
    21961 accttcttac aatagatggc gctggtggtg gtacaggcat gagcccatgg cgcatgatgg
    22021 aagagtgggg tgttcctaca ttctatctcc agtgtatggc ctatgacatg gctcagaaaa
    22081 tggcttcaaa aggcatgtgg gttcctgatt tagcaatggc aggtggtttt tcaactgaag
    22141 atcacatctt caaagtttta gcaatgggat ctccatattt caaagctgta tgtatgggac
    22201 gcgcactgat gatacctggc atggttggca aaaatattga aaaatggtta aaagaaaata
    22261 acctgccaaa gaatgttgct cagcatggcg caacaaaaga agagatcttt gtatgctatg
    22321 aagaactcaa agctaaatac gggaaagaag tggataattt ccctcttgga gcaataggtc
    22381 tttacacatt cagccagaaa ataaaagttg gtttacagca gattatggct ggcagccgca
    22441 atttcagaat tgataccatt tcaagaagcg accttatggc acttacagaa gaagcagcaa
    22501 aaatttctgg aataccgtat gtaatggatg cctataagga agaagctgaa aagatccttt
    22561 tagaaggatg tgattacatc taatatttat tgtatccatt taaaaagagg ccgtctcaaa
    22621 acaaaccatg gggcggcttt ttttagtttt tgatatttta aaaaaagggg gtgtcttttg
    22681 agacaccccc tattttatct tatgaatgaa atatgtactt cccgtcaaat gcggcaagag
    22741 ccttaattaa tttttacttt attcgtacat cttctcaatt acatctttat agacttgaat
    22801 tatcacgcgt ttctttgctt tctgagttgg agtaatcata ccatttgatt ctgtaaacct
    22861 ctggttcagt atggtgtagc gcttgacttc ttcaaatttt tccagtcgtt tgtttgcttc
    22921 ttttacttca ttatctatca gttcctgcaa acgtttatta ttgataaaat catctccaac
    22981 ttttgtacat atctgcatgc caccaatatt ttcatatacc agttttgact catcaaaagc
    23041 aatgccttca cgtttgaaca acgctataaa gtttgcaaag tttggcacaa tcaacgcgct
    23101 aatatatggc ttttcatcac ctacaaggaa aatctgttct acccagctac ttgttgaaaa
    23161 tagattctca atttttgctg gtgcaacatt tttgccaata gaagtacaga ttattccttt
    23221 tttcctttca acaaatttaa tatacccatc aggtgtcaac tctactacat caccggtttt
    23281 aaaccaacca tctttggtaa atgcctcttt agtttctttt ggcttgttga gatagccttt
    23341 aaatatacca gcgcctgaaa gctccagttc accatcagca gccactctac atttactacc
    23401 attggcttct ttacccatac aaccaggctt gcacgcagta attggattaa gcactgctgc
    23461 attgaagctt tcagttgaac catatccttc tacaacagcc agccccaatg cataataaaa
    23521 cctcagcaaa tcaggcgata tagccgcact tgctgaaaat gaatggtgga atctgtttcc
    23581 aaataaacca cgaaccttgg caaaaagttt atcagctatt gaatacttta ttttaagacc
    23641 caaaggcaac ttagatttta aatcaaactc tggatgcatg ttgtagcaac catttgcatc
    23701 tttgcggtat tcaagtgcct gtaatcccac accaattgcc cagtcaaaca gctttttctt
    23761 caatgtgctt tctgccattg cctgctgcat ggttatataa attttttcat acaatcgtgg
    23821 cacacagtta atccatgtgg gattatattt ttgcatatct tcaattaatg ttgcaggctt
    23881 atcagcatag catatcaaag atcctttagt taaggcaagc cactggcaag aaccgcggtc
    23941 aaatacatgc gatagtggca ggtagcatag tgtaacatca tgttcattaa catcaaaatt
    24001 atatttctgg aaatatgcaa ctgtaccctc taatcgtgat gaaccagtcc agtgcgtgat
    24061 aatagcacct ttcccctgac cggttgttcc agaagtatac agtattgtga accaatcatc
    24121 caattttaca ccatctttcc gtttgatgta tttcttataa tttgctgcat ttttccgcca
    24181 ctctctgcct atggcaataa aatcctttat gccatagacc ttcttgttgt cacctttaaa
    24241 tgatgtgtcc attacaataa cagttttaag atctttcagc tgtttccaga tccgttttat
    24301 ctgatccaat tcctctttgt tgccaacaaa tatcactgta gattttgaat ctttcatgat
    24361 gtatgtgact tcagtatcag ataatgtggg ataaattgtt acactgacgc caccagcaca
    24421 ggcaatcgca atatcaacct gtgtccacat atagcttgtt ggcgccataa ttgcagctct
    24481 gcctttgtgg agaaacccta ttttcaacaa tgcacaagca atatcctcta cacgctcttt
    24541 taactctgca tacgtaaaca ttccattatt gtcatcatac agcttactat taaatttttg
    24601 tgcaggccgt tcaggaaatt tgctgcaagt gtcataaaaa tactgaacca tcgttttaag
    24661 ctcacccata ataacccctc tgaatatatt aatttaaata taactattta tatatcacga
    24721 ctggaaggta tttcaataaa ttaattacat ttttccattt atgttattta ctgctatgca
    24781 atacaatttc cctgcatttt gcaagtgctt ttgcaatatg ttcaggggat ttgccaccag
    24841 cctgtgccag atcttttctg ccgccaccac cgccaccaat ctcttttgat accgttttga
    24901 taatagcacc acaatcaatt cctttatcta tagcaccttt ggtagctgca gctataataa
    24961 atgccttatt ttcctttgaa gaagctaaac acactaccaa agtatccgaa agctcccgta
    25021 tataatcagc cagtgtacgt aaagcttcag catccatatt ttgcagtgac gcactcacaa
    25081 acttcagact gttgatatgt tcagctttgg tatataactc cctggcaata tctttcaacc
    25141 ctgcagcttt tgccttttcc agttcctttt ccagatagga aactttttca agcatttcgg
    25201 caaccttctt ctcaatattt gattcattta cttcaagcaa ccccattatc cttcttacta
    25261 tcgcatgctc atgaatcagc cgttcatata ctcccttcag tgtcactcct tcaattcttc
    25321 gcacacctgc accaggggaa gactctcgca ttattctgaa aacaccaatt ttgcctgtat
    25381 tgtcaacatg cgtcccacca cataattcct tgctgaaatc gctgatagaa accattctga
    25441 caatatcaga atatttttca tcaaatactg ccatcgctcc ctggtttaag gcttcctgca
    25501 atggtataat tgcagtattc accggcaatg cagcccatat tttttcatta actatcgcct
    25561 ccacctgctg aatttcttcc tgtgtcattg ccttaaaatg ggaaaaatca aacctgaaat
    25621 gctggggatc taccagtgaa cctgcctgct ttacatgctg ccccaaaacg tgacgcagtg
    25681 ctgcctgtaa aagatgtgtt gcggtatgat ttgctcgggt aagatttctt ctgagaccat
    25741 caattgataa ccgtaccttt gtaccttctt gaagagttcc tttttccacc tgccctatat
    25801 ggacaatcag atcaccagat ttttttgtgt ccacaaccct gaatgttcca ctatcaccaa
    25861 taatgtaacc agtatcccca acctgcccac ctgactcacc ataaaatggc gtgtttttga
    25921 caaccagtat tccttcctgc ccctcattta aatgagtctt taagctttca aaatctgaca
    25981 gcacaataat ttccgattct gaatcatatt tttcatagcc ttcaaactgt gtgctctgaa
    26041 ccttttttaa tatttcacct acaaccccat caatcctgcc ttccacacct ttccagctct
    26101 gtcttccgcg gttacgctgt tcttccattt tatactcaaa accttcacgg tcaatacgca
    26161 atccattttc acgggctatc tcttcagtca tttccaccgg aaaaccaaat gtatcataga
    26221 gtacaaacac atcattcccg ctaattgttg attccccttt tctggttaat gttttgataa
    26281 tctcttccag tcggtccatg ccattttcaa ttgtttctaa aaagcgctct tcttcacctt
    26341 ttataacagc tttaacattt tctttagtgt ccgatagttc cggatattgt ttccccatgg
    26401 ccgcgaccag cgggtcaaca atggtataca caaacggttc atgaatacca atctggcggc
    26461 catacctgag cgctcgtcgt aagatacggc gcaatacgta gcccctgcct tcatttgacg
    26521 gataggcacc atctgatatt gcaaaagtaa gtgcacgtgc atgttccaca agcacattga
    26581 cagctatctg tgcatcgcct ttgtatgaaa tattccgctg tttaacaata taatcaacaa
    26641 ggcctttcaa ttcatcggtt tgatagatac tgtcaacatt ctgtaccagc gttgccaggc
    26701 gctctagtcc cataccggta tcaatcccgg tacgtggaag tggatgcaga acaccttcag
    26761 tatcctgaaa atactgattg aacactaaat tccagaattc taaaaaccgt tcacaatcac
    26821 acccgggttt gcaatcaggc ttgccgcatc caaatgaagg cccacggtct aaatatagtt
    26881 ctgaacaggg cccgcatgca cctgaatcac cggctggacc ccagaaattg tcttccttgc
    26941 ctaaccgtac tatcttttct tttggcaggc caatattttt atgccatata tcaaacgctt
    27001 catcatcatc tatatatata gaaacccaga tatcctcttt tggaaaacca ataacatcgg
    27061 ttgaatattc ccacgcccac tgaatagctt cttttttaaa ataatcacca aatgaaaaat
    27121 ttcctaacat ctcaaaaaag gtacaatgcc gcttggtctt cccaacattt tccaggtcac
    27181 ttgttcgcaa acatttttgt attgacgctg cacgggtata ttccagttca acagtaccgg
    27241 caaacatggg tttaaactgc accatgccgg cagttgtaaa cagcagtgtt ggatcatcct
    27301 ttggtataag tgaacttgaa gggacaattc tgtgatcccg ctctttaaaa aatgtaataa
    27361 atgatgtccg caaatcatgt accttcatca tactgtgcat tccttataaa taaaaataaa
    27421 atcactttaa tgttcaaaac aatgtaaaaa aacgatgtta caatttcaat atattaatta
    27481 tagtaaattt caaaataact tctgaaatta ttcctgcata tcatgtatgt cttcataatc
    27541 atcgccaaat cgcttcataa cttttttaat aatttcccag ccaaatcctc tttgctgcaa
    27601 aaaggccact acctttacat gagggttgtg tttttctttt acctgtttat atttttttaa
    27661 tgccagctga tacacctgct caatatcagg ctctttatcc actgtttttc taatagcttt
    27721 tttaatgatc cccttatcaa cacctttctc aaaaagtttt ctttctaaca ttttattgcc
    27781 aataacctta tgttccctga ccgattgaat aaagtgcact gcatattcat aatcattaat
    27841 gagtccccgt tcagtaaaat attgaagtac cctgctgata gtagttttat caaacccctt
    27901 tttcatgaga tattttttta tatcgtgttc agtacggcta cggatgctga tataatacaa
    27961 cgccttttcc ttacactcaa agagcatgga ttcttctttt aaatgattat atgcgtcaat
    28021 atcaatggcc acaccccttc gtaacaatcc tgaagggaca gaatcattgt ccactcgaaa
    28081 atattcgcca gtatccagtg ttatccgaat atagcgatca gtatattcaa tatcctgtat
    28141 aatcattgca taacacacat attaaaggcg ttcatgcaga cataacaaaa aggatgccac
    28201 ctggcatcct ttttgtaaag tatatattgc attccatttt ttatcgtttg gaaaactgga
    28261 atcttttccg tgcctttttc tggccatatt ttttacgttc aacctcacgt gaatcccttg
    28321 taaggaatcc tgcctgccgc atagattttt tatacttttc atccagcgct gacaagcatc
    28381 gtgcaatacc cagtttaaca gcgccagcct gccctgtcca tccaccacca tcaacagtgg
    28441 caacaacatc aaatgaattc agtaccccca cctcttccag tggctgtcgt accacaagct
    28501 ccagggtttg cctcctgaaa tactcgttta ttggtttgtt attgattatt atagttccat
    28561 ttcccggttt taaccataca cgcgcaaccg ctgattttct tcttcctgtt gcataattta
    28621 ctgcacttgc catttttata acctcatttt tagtaacgat aatttttctt tacgcaatta
    28681 aaactttaat tctaaaggtt caggtttttg tgccttgtgg ggatggtcgc tcccggcata
    28741 tatttttaaa ttacccatta actgtcgtgc taatttgttt tttggcatca taccttttac
    28801 tgcatggtac aatacatatt cgggcttttt ctcaagcatt tttctaatat tcacaaatct
    28861 gctaccgcca ggatactttg aatggaagaa atattctttt tcctgtggct tcttgcctgt
    28921 taaaagcacc ttatcagcgt ttattactat aacattatca cccatatcaa ggtgaggcga
    28981 gtatgaaggt ttgtgtttcc cccgcaacac atgtgcaatc tcagttgcaa gtctgcctaa
    29041 aaccttacct tctgcatcaa tgatatacca ctttttctca atctgagatt tttttaaaga
    29101 aaatgtttgc tgcaaaatac ccattatatc ctctctataa tactgattat ttcgctgtta
    29161 tgctaaaatt atatagatta caagtgtagc acgttattta tattaatcaa taaacaaaaa
    29221 tcaataaaaa tatactgttt tcaaaattgg tgttcaaact tatttttaca tgataatttt
    29281 gtcaaggaaa ttaagcattt ttttacagaa taattttttt cgatagttat acaaatcgta
    29341 tgtattcatc agtaatcata aatgtacagg gagtaatacc tacatattct ccttccacat
    29401 caactggaag ccctggagtt aagatatcta ttcttctggc atccacatct tctacgtaac
    29461 gaacattaac atgagtacca gaatatactt ttggaaacaa cagtaagaat atgatcttct
    29521 tcatccattt aagcaatatc atacgagctt tattgcttaa ggtatcagaa tgtggggata
    29581 ctttcatgcc gcctgcataa tatttgcttc gtgcaaacat tatcataagt acatcctcaa
    29641 tctttacttc accatcaata tttattttta ccttcactgg cttaacagta aaaagtgttt
    29701 gcagcacacc tataacatag ccaattttac ccagccttct atatctgtca aaaattgacg
    29761 ccacttttgc cccaaaacca gcatctccaa caccaacaaa atatctaaag ttttcttcag
    29821 taattacttt tgataacctt attttaatat caattccatt aataattcgt tggtaaatct
    29881 cttcaatacg gttttcaatt ctttttgcta ttccaaatac tctacttaaa tcacaccctg
    29941 aacctccata agttggtgcc acaacaatat cagttctacc tgctaaacca tttgctactt
    30001 catttatggt accatctccg ccacacacaa taataacatc acttaccgag gcatgagcaa
    30061 gctcagttgc atgcccacga ttctttgtaa aaaaaacctc atgattaaag tacttatcaa
    30121 tacaatcacg tagatgatga taaaccttta atccagaacg agaagcagga ttgactataa
    30181 tagtggcttt tggtataaca tcatttttat tggaagccat atactgaaat ccatgttaag
    30241 gagttacatt caattcaatg tgattatcaa gcaaaatatt aaaataattt taaaaacatt
    30301 tactatatag ataaatattt tcaatatatt tttaactaat atggcaatca tatcaaaact
    30361 ttttatatta ccatcaaaat aattgtatca tgtcataaaa tattcataaa accaatatat
    30421 aaaaaagcag agactttcat ctctgctttt ttaataatta ttttttataa cttaaaattt
    30481 tttcatattc ttctgatata tagcttttgc ttcttttact gttctttcaa taacttcttt
    30541 tacggttggc acatcatcta ttaacccctg acactgccca ataagctgca caccataatc
    30601 aagatcgcct ttttcagttg ctacctttat tttatcaaat gcagtggcaa aatgtgccag
    30661 ccgaatcatc attcttggcc cctgcagcat aacgcctaac gctaatttaa aataaggcat
    30721 attcatcatc ttagctatct tatatgattc taaggctgca tgaggcaaac tcatgccttt
    30781 tcttatagct ttttcagctg atttggtttt tagcacacga cagtatattc catcaaatct
    30841 attggaatag atagtatctt caatgcctgc tttaagctgt gcttcaatgg ttttcttatg
    30901 taatggactt tcctttgtca ttgacaaacg ggtacccatt ccaatacctt cggcacctaa
    30961 agccagtgct gcagccattc cacggccatc tgctataccg ccaacagcaa caacaggaat
    31021 ttttacctta tcaactatcg ccggaacaag aacaaaggtg gtaacctgcc caccatgagc
    31081 agctgcctca tgcccggtta cctgcagagc atcagcaccc tgtttttctg ctgccagagc
    31141 atgtttttca gtaacgacag ttgcaataac tttgccacca tatttatgtg cacgttctgc
    31201 aatccagtca catttaccca atgatacatt aatgatagga actttttcct caagcatgac
    31261 ttcagcattt tcgcgtgcac ctggcataat gagggtacat cctgcaccaa aaggtttatc
    31321 agtaagctca cgtatccttc tgatagcctg tcgtgtttgc tccggtgtca atggacctgt
    31381 tgccagatag cctgtaccgc ctgcattaca tacagcagct accagttcag gagtgctaat
    31441 ccagctcatc ccgggaagga cgattggata tttaattcca aacagttcag taattcttgt
    31501 cttcatacaa taactcccca tttactaaaa attattattc tcattaccta aaaacataac
    31561 accgttatac tactattgta gcaataaaaa ttttatatac aaaaaatact aatttaaact
    31621 acattattcc agatacatct tatcaatgat gtcattgtat cgttgctgta ttactttgcg
    31681 tctaattttc attgttggtg taatctcacc attttcttgt gtgaattcat gtggcatcaa
    31741 ggtaaacttt ttaatcttct caacctgtcc caagctttca gttagttttt caatcttatt
    31801 gttgtagaat tcaataattg ccggatttga aatcaattcc tgccgtgaag aatactgaat
    31861 tccttttgat tttgcatatt cacccaagat ttcaaaattg ggcacaatca gggcactgat
    31921 atattttcgc ccatcaccaa ccaccacaac ctgctcaatg tagagatctt cgcctaacat
    31981 tgtttcaatt acctgcggag caatattctt accaccagca gttataatca ggtccttaat
    32041 cctgtcagtg atgcgtatat acaactttcc atcatgttct tcaatggttc ccacatctcc
    32101 tgttttaaac cagccatcat gtgtaaacat ttccctggtt gcttcaggtt tatttaagta
    32161 accctgcata cacatggggc ctttcagctg gatttcacct tcatcagaaa ctctgacatc
    32221 aacaatatca agaattggac cacagacacc aggataagct gttgcagcag tcccaacggc
    32281 aactgggaaa aattctgtca atccataccc ctgagccagc gggatgccca atccattgaa
    32341 aaatgcattg atctcggcag acatagctgc tccaccaaca tggaaaaccc atacattgcc
    32401 accaactatc tgccgcacct tttttaatac aagggcgttt gcaaaaaatc tttttatacc
    32461 aagccatggc ccaataaatt gtttggccct ttttctagta tcgtattcaa tgccgatagt
    32521 agttgcccaa ttaaacaatt tcttctttgc aggtggcgct gtttctaatc tgtcaaatat
    32581 ggtagcatac atcttttccc ataaacgggg aacgctgcac atataatgtg gttttgacag
    32641 tggcaaaatt tccataatat cttttggatt acgacagtaa tggttttcac cacctttcag
    32701 caatactcca tatgtccaca tgcgttcaaa cacatggctt aatggcaaaa atgcaagaga
    32761 taccataccc ggtcctgctt cggggaagcg gaataccgat gcatagatac cagccaggaa
    32821 gcttgcatgc gtatgtaccg cacctttagg attcccggtt gttcccgaag tgtagattat
    32881 ggtaagcatg tcattgctcg ataactcatt aatacgagca tcaactgttt tttttgcgtt
    32941 atcactaact tcagttttta ctatctcatg aatataaaaa ctattctcac catttactat
    33001 cacagcatcc tgaacagata caacaaactg taagctggtt gagttcatta tctgaagaat
    33061 cttatcatac tgttcctgat caccagtaaa gattgctttg atagaagcat catcaagtat
    33121 atactttgcc tcatccctgg agttagttgc ataaattggt accgggacac agcctgctgc
    33181 ctgaattcct aaatcagcta atgtccattc agggcaattt tgtgaaaaga ttgcaaccct
    33241 gtcacccttt tgcaggcctt tagcaattaa aaaatgagct atcgcataca ctatttttgt
    33301 cagatctttc caggtatagc tatacttctt gccatcaatg aatcccgata gtgcggctgc
    33361 attaccacgt ttctttgcac gtgataacac catgttgata acactttttt cattggtata
    33421 aaattcctta tatccttcaa gtgttgcttt ctgcatagat accccacccc atgataaatt
    33481 tttattagct cctgtaaaaa acgatggcta ctatttaacc ttttttgcca gttgcttttc
    33541 aatctccact gcttctttga ttattctttc cacaacctgt ttcactgttg gtgtatcatt
    33601 aataatgccg gtaacctgac ccaatggcaa aacaccttta tcaaaatcac catccatggt
    33661 tcctatcttg aatgcatcaa aaccaattgc catgcgtgcc atctgaattg ctcgttgtgg
    33721 tcccataaac ataatgccaa ctgcaagttt taaccatgga tagcctaaca tatcagcaat
    33781 tttctttgaa cgaataagag agctcagtgg attaagatgc tttttcatga gtcgctcaac
    33841 agccttagat ttcataaatc gtgcaggcaa tccatcaatt tttggagtat acacggtgtt
    33901 gtatacttca ctcttaatac ttgcctgctt tgcactttcg gccaccgggc tttccttggt
    33961 attcataaag cgggtgccca ttgatatgcc ttctgctccc agtgcaagag ctgcaataag
    34021 ccccctgcca tctgcaaaac cacctgctgc aataacagga atatttactg cctgtgatat
    34081 tgcaggaatc aacaccagcg atgttatggc accaccatgg cctgctgctt catagccggt
    34141 tactatcagg gcatcagcac catcgcgctg ggctgccagt gcatgtttat gcgtggtaac
    34201 tgtcgcaatt acttttccac cgtatgcatg aacagctttt gcaatcttat cacctttacc
    34261 caatgaatag tttatgatag gaactttttc ctcaatgagg acttcagcgt ttctctctgc
    34321 accaggaaaa tacagcgtaa catttgctgc aaatggttta ttggtaagtt cccgtactct
    34381 tcgtactgcc tcacgggtct gttcagctgt taacacaccc gttgcaagga taccaagacc
    34441 tccagcattt gaaaccgcag caacaagctc aggtgtactt atccagctca tacctgaaag
    34501 cacgattgga tatttgatct tgaaaagttc tgtaattttt gttttcattg tgtttctcct
    34561 caaatgtata ttcttattat gatacattat ttccgctaaa agcggatgaa ttataccttg
    34621 ttatctatct cctcttattc tatcgcaaac ataacagagt tatctaaaaa agcaagaaat
    34681 atatatactt tttgtcaaaa cttttatata aaaaattatg tagttgaata atttatacgg
    34741 cacaatacat aggggagata ggcatcgcca ccccattccc gttctgaaga aagtactatt
    34801 ttatcattca acattgttgt aacatcacca cttaccatgg aatttgttaa tcgtcctttg
    34861 acttcaccct ggacaaccag ccatgccagg tccagattgg ctgaaaagtc tcccattaat
    34921 gtatttgatt gaccaaagcc aataaactga tctataataa ttcctgttcc catttgagct
    34981 atcatatcct taaaggacgt ggtgcccggt tcaacaacta tattgctaaa actttcctgg
    35041 ggtaagatag cataccctcg tgaagcattc ccggtaggtt gtacgtgcaa aagatgggca
    35101 ctctgcaaat gagcaatata ggtcttaaca tacccatttt caataatagt tttactttta
    35161 gctggaattc cctcatcatc aaaagggtaa ctaccaggtg caaaatcaat taacggatta
    35221 tcataaactg taaactgtct accaaaaacc ttttctccaa attttccaat atatcgtgaa
    35281 attccacgct caattgaacg ccccgtcaaa ccctgcagaa ccacacccat tatctgacca
    35341 aacgcctttg gagtaaaaat tacataataa ttaccaaatg gcattttgac ggtttgtttg
    35401 ctaaaattca gtttttgtat aattcgttca gaaagctcat gtatattaac atcactgttc
    35461 caggtaatac tttcatatac ctggacacgg ctaccatcag gcaatattcg caaggcagat
    35521 attgttgaac cctgccgcga atagtggtac ccactatcaa aacccctgga attaatcaga
    35581 tgtatatatc catccacaca ccccaagccc gcatcaacca tacagtcact gtatgctgaa
    35641 cgaattgtat ccacaacgtt ctgtaatgtt tggagatagt attgggtgtc tttattgaat
    35701 atgttcccat catagcattc aacatgggga gtgggttgtt ttgccggaag atcatattct
    35761 tcaatttcac cataacgtgc tgtttcacgt gcatattcca tcacagaatc aagcattgca
    35821 atatcattac aaaatgaaaa acccgttttg ccatctatat tcatcctgat gccgtatccc
    35881 tttatttctg attcacgcac accataaaat acattatttt taaatgaata tgttttatgt
    35941 actgcatact cagcaaatac atcccaccag cgagtttttt gttttgcaca ggtttcctgt
    36001 attctactta gatttagttt ttctacttcc agcacctttg cctcctataa gaacattgtt
    36061 aatcaatata tgcggcccac cggaagaaac tggcagtgga gcctgaccct gtttgccaca
    36121 accgcccagt ccaccaaaca atgtacaatc gttagctatt gcttcaatgt tggcaagagt
    36181 cacaaataca ttccccgtaa gcatgcactc acgtaacttc ttggtttttt tacctttttt
    36241 tacctcatac gcataggcag gcgtaaaggt aaacatttct aaatttgttt gacctcccag
    36301 agcaccaacc acatagacac cattccacag cttttcaaat aattcctcaa cggtagaatt
    36361 cccattttct atatacgtat ttgtcatacg cacaatagga ctggctgaag ccgttatggc
    36421 acgtgcattg ccggtcacct cttcccccat ttttaccgct gtttcccgtg aatgaagacg
    36481 gctgtgtaaa attccatttt ttattaaata tgttttttca ggttttatac cctcatcatc
    36541 acaggggatg tatcctgcat acggaagtgt cccatcatct acaacattca gtatatccgg
    36601 gccaaaacgc ttaccaataa ccattatctc tttcatccgt ggattttcat atacaaaatc
    36661 tgcttcagac agatgaccaa atgcttcatg aataaaaaca ccagctaatt ttggatcggc
    36721 tataacatta tatacaccac tttttatctg tggtgcctgt accagctccg ttgctgtacg
    36781 cacaacttct tctgccaccg ggtgtttatc ctttactatc tcaaagcctg catagttagc
    36841 atccgattcc ccatatggct gaatgaccat cccatctttt gcaatagccg atagcgacaa
    36901 tccacagaaa gacctgtcat attctacaat gctaccatca gtattacata acacatactg
    36961 tgtcagtgaa tcacggtaca acacattggt attttctata agattactat ccttcaatat
    37021 gtcattgtac tgggtaagca cgttcatctt ttcttctaag cttactgtat ttggcggaat
    37081 aagcacattt gttttttctt taatcacatt catctgtaat gaggatagtg tgggtgcgtt
    37141 ttcttttttt tgtatgttcg atagctgtag cgcctgttgc atggcataat caaccatatc
    37201 aagagaagta aatgcaaaaa atccccaaca ccccttatta taaacgcgaa ccgacccctg
    37261 cgttgaagtg cctaccttac aggtttctat cattctttta gaaaggtgta ttgtggtaga
    37321 aaaactttca cttatacgaa tttcagcata ttctgtttta atgcttttta actttttctg
    37381 taaatccatt ggatcaagca tggtaacaat ctccattcaa taaaaaatct atagtttttt
    37441 tcatcatgca actaaagaaa acaaaatata aaagcgtatt gctttgccat ctctaaaatc
    37501 tttgcctttg ataaaagatg taattcaaaa tgctatccta gaaaatccct gcatgttggg
    37561 atgataaaaa cgtccctccc ataaaaataa agattcttgc cccaatttta tccccgattt
    37621 tatcagggtc agaatgataa caattgttta atttttaaaa agcttagtaa tcctcctgta
    37681 ctacattatt cactgcatac agtattgcct gtgcaagatg atatgatact gtgcgccact
    37741 gtcttttact gttgaaaaaa atctgctggt tagcttccag ctcaattccg cagtaactat
    37801 cgccatactg cttgcgcaac cacacagtca cgccatcacc gcatcctcta aatggataat
    37861 taaatgcaac ccgcaaagga tatgcttgca tcaacgcttc tttaatcttg ctgcagaata
    37921 gcgcttcttt agggcggctc tggtcataca gcaatcctat atcagtatag cgtttaaaac
    37981 catataattg cggcgtaaat gaatgtgctg caatatgaaa tacagcatct gaactctgta
    38041 gcatggtgtt aatagctttt tcaattttat tacgatatac agtataataa cgcttaacta
    38101 tctcattttt taaaaaaata ttgctccctt gtatcaattc tgaaaaaagt tcattgcttc
    38161 ctattgacct gttacaatcc accactaacc gtgtaacagt ggtatacagc agaggtgctt
    38221 caagcgtctt tgcaaggatg cgtgccatct gcaatgcgcc aatatcatac gcccagtgag
    38281 tggaaaggat ggtagtgtca gtgaaaatat cgcgcaatgc ctcagggaca cgattgcctg
    38341 catgttcaca ggttattatg atggaaacgt ttttcatcag tgcctcaaaa tttttatata
    38401 acttgacatt ataccatact atacgttttc tttggtcaat atatattttt tacaggagta
    38461 gtatctatgt taatcataac cactatcatt ggattatcag ctggtattct aggaggatta
    38521 atgggcattg ggggtggcat catcatgatc cctgcactta tatatttact tcattttaat
    38581 cagcatatgg cacagggcac aacattagct gccatgatac ctcccattgg gatactggca
    38641 gcatatgaat attacaaatc tggcaatgtc aatatacctg tagctataac actagcctta
    38701 gggtttgtta ttggtggcta cattggtggg aaatttgctg tatcccttga taacgaaaca
    38761 ttgcgccgtg tgtttggtat aatacttttt attatgtcgt tacatataat cttcagtaaa
    38821 taattacgcc ttatatccac ccaagttagc taatgccaca ctgccaggac ttattgtatc
    38881 aggatcagtc atgacattga ccagcgccac agtattgctt tgcaatgcat caatgatagc
    38941 tggttttatg tcctctggtt tttcaactac atatcctttg ccacctaaag cttctaccag
    39001 cttatgatag ggaatatttc caataaatgt tccttctttt acagggtgcc ccatccgtaa
    39061 aacctggctg tgccgaatca tcccccaacc aaggtcattg ctaacaacta tagtaatgga
    39121 aagtcccttg cgtatagcgg tttctatctc cataaagttg aatcctaaag aaccatcgcc
    39181 agtaaaaagc acaacacgtg agttcttgta atacagcttt gcagcattgg caaatggaat
    39241 acccactccc aaacacccat acaatcccga atccaagtat gttccacctt tttttaccgt
    39301 ccgtgtcatt cccatccata tctgagcatc tccaccatca ctgaccacta catcatcttc
    39361 cttatccatg atctgattta cttcatatgc aacacgcatg gcatgaatag gcactgcatt
    39421 actcagccat tgttgctgtg cctgcttttt gctttcttca tcagcttttt ttagttcaag
    39481 tacccattct ttgaattgct caggtaaatt caattgtctt tcttttacta ttttattaca
    39541 ttcatcaata aatgaactga catcacttag tattccctta tatatactgc ggtttcgtcc
    39601 tatctcttct ggttcaatat ctacctgaac cagcgttgca tctttgttaa agatgtctcc
    39661 aaatatataa tataagctaa ctctattacc cagcaataac acaagatctg cgttaaaatt
    39721 ggcataaaga gctgctccgg ggcgtattgc aagggatgat tcaaaacaca gcgggtgtgt
    39781 atctgctatt acacctcttc cggcagcgtt agtgaagaca gggatacccg tactctctac
    39841 aaattgcagc aattccctgg cagcatcggc ataccatgca ccgctgccgg caataataat
    39901 aggctttttc ttgtgctgca atagctcaat tacatcatac gctgcgttgc tatcgcacgg
    39961 ttttgaatat accaatgtat ttgtcttttt tacttttgtt tcatctggcg ctgcgttgag
    40021 aacatcaaca ggcaattcta aatacaccgg tcctggcctg cccgaacacg ctgtgcgata
    40081 tgccatatca acatattcgt acacacgctc aggtttatga cacacaaaag ccttttttac
    40141 catgggttca attaccggca actgcttcat atcctgtaaa tcaagctttt cattgctttc
    40201 cagccccaca catccagcaa tgagtaagag tggagcattg gataaccgtg cattggcaat
    40261 agcggtaaga gcgttggtaa accccgggcc ggctgtaaca agggcaactc caggctttct
    40321 actcaagcgc ccccacgctt cggccataaa tacagccgcc tgttcatgac gggtatcaaa
    40381 tattgtaatt gatgagtttt ctaaatgttc atagatgggg gtgatatgtc ccccacttaa
    40441 cgaaaagata tagtcaacct gtttatccag taatgcttca accacaagtt ttgcaccatt
    40501 agccatacaa tcctccatac tgttataatt ccattaattg cccgccacag acatttattg
    40561 cctgccctgt aatatatgaa gacgcctcag aagctaaaaa tacaacaagc ctggcaacct
    40621 cctcaggtgt gccaatcctg ttaagcggga tactctgaca catgagcttt tcccgctctt
    40681 ctggtgttgt gttgaatact tttgcttcta attcaaaacg ccagcgttct aaatctgtat
    40741 ttatttgccc tggacaaatt gcattaaccc gtataccagc accagcaagt tcctttgcca
    40801 tgaccttggt gagcataata acgcctgcct tggaaactga ataagcaccg ttaaacattg
    40861 cgggaaattt ccctgcccgt gaagccgtat tgattataga accaccctgt ttcataagcg
    40921 gtacaatggc cttagaaacc ctgaatgttc catgcagatt gacatcaact gttttaagcc
    40981 aggcttcatc atcataggta tgaatagcac tgggaacacc aaacgttgca cctgcattat
    41041 tacacaggat atgtacagaa tcaaacaatt ctgaaacttt gactgcaaat tcattaattg
    41101 actctttatt ggtaacatca cagagtaatg cttgtgctgt aacggaatat tctttttgta
    41161 attctgcagc caactgctgc atctggctta attgaccagc catcatcgtt gtatcaccaa
    41221 tggtgccctt acctaaatca gctataatga tattagctcc attttttgct agttcgcgag
    41281 caatagcaaa accaatccca gttgacttac cagcaccggt gataaccgca tttttatttt
    41341 tcagatcagg atacatgaca acctccaatt aatcgaaaat gacatatcat attattttat
    41401 ttatgtcaat aatgaattta tcaattactt caatggaagt tacaaaaagt aacggtgaac
    41461 ctgttagtta ttaaaaactc ttacaaaaat ataattgcaa attgtaagca catgactaca
    41521 atagtagtca att
//
LOCUS       DAORYL010000017        41495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_750_length_41495_cov_13.698673, whole genome shotgun sequence.
ACCESSION   DAORYL010000017 DAORYL010000000
VERSION     DAORYL010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 41495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41495
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_750_length_41495_cov_13.698673"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1105
                     /locus_tag="PLV81_05115"
     CDS             164..1105
                     /locus_tag="PLV81_05115"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HOM09512.1"
                     /translation="MKKIVLYVAIVISALITFNAYSQEYLATGIMPFTSSSKNQSEIV
                     VSQLSKILQEYQFIRLIERSQMQKLIGEIEFSQSGLVNEQSALQQGKILGVKVMIFGT
                     IDNNNTVSARAVYTETGRVIASSMQSSQNIALLGKELAYGIESFLARENLKKMRNDSP
                     DIKLDFWIETKDGKRINPNDGLKIGQSVKFKFRANKSGYVTIVDIQPTGEVVILFPNQ
                     FNMNNYITADTVYSIPSETDGFELTVTEPAGEDTITLFFTEKKVDWLDMKKLQGEGFQ
                     SVEPAERFTVTRGFSVTATQLNTKQWESVVINVLVKK"
     gene            1117..1509
                     /locus_tag="PLV81_05120"
     CDS             1117..1509
                     /locus_tag="PLV81_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013523108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOM09513.1"
                     /translation="MKSIKPGRMTSFQGFVGSIIAIVFGIFWLFLVTSFMFENEDIPQ
                     AIFFAFAFFGVVFIVIGIVNAVVEYKNAFSKNRYSLLDVVDSSEEQDPFHFKNNTTKL
                     DTINYCPHCGTSIQKEYTFCPGCGTKLK"
     gene            1529..2314
                     /locus_tag="PLV81_05125"
     CDS             1529..2314
                     /locus_tag="PLV81_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HOM09514.1"
                     /translation="MTQKITVEKKGHILLIGLNRPEKRNAFDVDMYWDLAKAYGTLDA
                     DDELRCGVLFAHGEHFTGGLDLVQWVEPFSRGEYPALPSDSKDPFGIDEDNRVSKPIV
                     AAVQGICFTVGWELMLATDVRVAATNVRLAQIEVKRGIYPVGGATVRLFEEIGWGNAM
                     RYLLTGDEMNAQEAYRLGLVQELVEPGKQLERAIEIAEVIAKQAPLGVRAILRSARLS
                     RVEGAKAALARLLPDLLPIMKSEDAQEGVKAFIERREANFTGK"
     assembly_gap    2346..2445
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2446..2683)
                     /locus_tag="PLV81_05130"
     CDS             complement(<2446..2683)
                     /locus_tag="PLV81_05130"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HOM09515.1"
                     /translation="MELVLIIMAILCAIGGILGSVFPILPGPPLSFFALILFNFSGFA
                     TFSFTFLLIAGTIAAILTVLDYILPSWIVNRFGGS"
     gene            complement(2759..3433)
                     /locus_tag="PLV81_05135"
     CDS             complement(2759..3433)
                     /locus_tag="PLV81_05135"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HOM09516.1"
                     /translation="MKKIIYGCVSLLLCCSIGILYAQDEKAKEAQKVLEQEKQLSDQV
                     YKEVIIEDFETTDYTDKNISLRVVKDEKAGVSMRTDYPAPVQNSKRYLGVKVFAKSGS
                     VLTIIPAKPLIIDKYCKYINMWVYGKNFSGELSILVKDANGNIKRLVLGKLNFLGWRK
                     LSVPITDEIAQEDKYLAQKRNIEIVKILYNPSTLERLPAWNYFYIDDITAIVREKYVD
                     RQSDEW"
     gene            complement(3539..>4582)
                     /locus_tag="PLV81_05140"
     CDS             complement(3539..>4582)
                     /locus_tag="PLV81_05140"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOM09517.1"
                     /translation="KKQHTLEGLKTNTLKCMTINVWSGLDYIGTFKMGEYESPDLREK
                     RYQAFLKEIARYNPDIIAINEANFLPDYVKRLANDINYDYIYHVGVAGLKIGRWGIPV
                     NLKEGDAILARKGLQLQQVGHKQLSGGGIITNYFSFHTEDATQVLIGRVIVNTKPVYV
                     AVTHWHASPFDTPHNRMLLKELQQKYGYTDTEYQEAIKKLEADNTWRKNEAKLMAEYC
                     KAIVPEHAPLIVMGDFNATPDSPEMQYFLQQGFRDTHIHHSNTDMYTWNPLKNLNIQK
                     YYKVDTNKKFESPYTHVSKIHELEPKRIDYIIVNTSIPPDKIINSNICCDTIYDGIHL
                     SDHFGVYAEIRID"
     assembly_gap    4583..4682
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4683..4876
                     /locus_tag="PLV81_05145"
     CDS             <4683..4876
                     /locus_tag="PLV81_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09518.1"
                     /translation="LSIEQEIHSLWPRMTITIAFNVIMNNNDFFILIMSITFQNRFPI
                     TIAQNIVTKLLIEFQKAIV"
     gene            4998..6644
                     /locus_tag="PLV81_05150"
     CDS             4998..6644
                     /locus_tag="PLV81_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09519.1"
                     /translation="MKRISLHRLYMKYGGLRRILHLISVSVFVVFLMSTCALAGNNFD
                     WFWFVYEQGEYYSSTYTVYRPLYLKNNYRNASFQASLMPVLYWKYATPRQQTAKGVFG
                     FYESVDYWGLHHYENDTGIFPFLLYGMSTDENDRYLHVWPLGGTIKGKLATDSITTYI
                     FPGIALFFLYPTSSYYVLAVYLVASIVPVYVHYVSKDYDAYGILWPLIQWGKSPVRDD
                     MRIMPIYAHSKKKGFYDTASYGLLYNVRKEYIGNDTMHTVFFVPLYARRWLESGKAES
                     SSLLWPFFSWGYNDKHGNLEINFPWPFVQIRDCDDPFIYKRIYFPFYGKYIERNKTTF
                     FITPLYFVLEKQGTGYESQYSIYGILFWYFKRDYDNEHQRYGKHWRFYKLWPLFHYEY
                     NDRGDSSFAFFSIMPFRDEEGYERLYQPFFSIIEYSRQSNGDRRFGLLLRTYFQSWND
                     NYFAMKIPFIISYSEKNNAMNHISLLLSFFSYTNDDTVQELCIGWIPVYRNVKDKKAY
                     EARMKKLASVRNAEGSPLIQDLSYLYVSSLPGATVYSLRF"
     gene            6722..7522
                     /locus_tag="PLV81_05155"
     CDS             6722..7522
                     /locus_tag="PLV81_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOM09520.1"
                     /translation="MVEKIKHTVINVIRDIGANTLNIIAMMGYCMLLFLEVVYYSKAA
                     FEKRREILKQMYYAGVKTFIVVSIVALFTGMILALQTGLELKPYQQQALVGNIIIATL
                     TREMAPFVTAIILIAAVGSTMAAEIGTMTVSEEIDALEMMAISPVKFLVMPRVIALAI
                     MLPIATIYTNLLGSIGGGIVAYFQLDVSFDVYYTHILDSLHFKATYVGLFKALVFGMM
                     VSTISCANGLRATNGALGVGKATRNSVVSSFLMVLIVGYYITALFYGK"
     gene            7552..8298
                     /locus_tag="PLV81_05160"
     CDS             7552..8298
                     /locus_tag="PLV81_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002763919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOM09521.1"
                     /translation="MIEFRNVTKILSGTKVLNNLSFKIEKGETFVIIGRSGTGKTVTL
                     KHIAGLLEPDSGQILVGGEQMNGISKSQRDRLREKIGFVFQSGALINWMTVEENIALP
                     LIEHKLYPPDEIKKIVEEKMDLLQLTSARDKMPAEISGGMKKRASLARVLVRNPEIIL
                     YDEPTSGLDPVMSSLINTIINQLKKDYTITQVVVTHDMDSAYAVGDRIAMLYDGRIIQ
                     CDEPDGIRNTDNPIVRQFISGSLEGPIDVL"
     gene            8323..8976
                     /locus_tag="PLV81_05165"
     CDS             8323..8976
                     /locus_tag="PLV81_05165"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOM09522.1"
                     /translation="MKQEIAVGIIVIIALTVLGYFTIIMSGEIFETHTYYPMTVVFKD
                     VEGLSADDKVRVNGVLSGYVDTVELIENNVIVTLRLYNHFTLYENYEIMVRNETALAG
                     KYVSISPGTPMDENGKQYAVITTRDNLIGTSVPDPFTMLSRLIADNRGNVNATVKNLR
                     DITDKINSGKGTLGKIINEDTVHGQATDLIKQLQDTIEDTREQAPITSFLRAALTAF"
     gene            9143..12463
                     /gene="carB"
                     /locus_tag="PLV81_05170"
     CDS             9143..12463
                     /gene="carB"
                     /locus_tag="PLV81_05170"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOM09523.1"
                     /translation="MPRRTDIKKILIVGSGPIVIGQACEFDYSGSQACKALREEGYEI
                     VLVNSNPATIMTDPDMADKTYIEPITHDIVARIIEKERPDALLPTVGGQTALNIAVQL
                     HESGVLQQYGVELIGAKIDAIKKAEDRDLFKKAMLDIGLQVPNSALASSIDEALKILE
                     TIPLPIIIRPAFTLGGTGGNIVYNQEDFVELVRKGLDESPISQVLLEESVIGWKEFEL
                     EVMRDLKDNVVIICSIENFDPMGIHTGDSITIAPQQTLTDKEYQNLRDMAIKIIREIG
                     VETGGSNIQFAVNPKDGRVMVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGLTLDE
                     IANDITRETPACFEPTIDYVVTKIPRWAFEKFEGADTTLGTQMKSVGEAMAIGRTFKE
                     SFQKALRSLEIDRYGFGSDGNLKLDALVDAIPERQRNDIIEQNLRIPREQRVFYIKKA
                     LELDWSVEKIHELTSIDPWFLHQLKELVEAEREFVAQAKQGLQKENILKMKQLGYSDR
                     QLAFLIHKDELKALYERGLDAQKDYSKRLNELEDSIRKFRFEQAIVPGYRLVDTCGGE
                     FEAYTPYYYSSYDDEDEAKHTTKRKVMILGGGPNRIGQGIEFDYCCCHASFALREAGI
                     ESIMVNSNPETVSTDYDTSDRLYFEPLTLEDILHIYNNEKPDGVIVQFGGQTPLRLAR
                     RLEANGVKILGTSPDSIDRAEDRQRFAEVVNKLKLNQPENGIAFTREEAIAQATRIGY
                     PVLVRPSYVLGGRAMSIIYDEKSLLEYIVSAVELSPEHPILIDKFLEDALEIDVDALC
                     DGTDVFIGGIMEHIELAGIHSGDSACSLPPVSVKPSMMQTIIETTTALAKELNVIGLI
                     NIQFAIKNDVLYVLEVNPRASRTVPFVSKATGEPLAKIAVWIMLGKKLKDFGLTYMKK
                     IPYVNVKEAVLPFNKFPGVDTLLSPEMRSTGEVMGIAKHFGEAFYKAELGAGDRLPLS
                     GTIFLSINQRSKEELLEEMRLLHQEGFKLIATEGTAAFLNEHGIPCERVYKVSEGRPN
                     VVDIIKNKQVDLIINTPTGKRPKEDAYTIRQAAVRYRVPIITTLAAAKAAVQGIISMK
                     KAGQFTVKPVQEYHREVQ"
     gene            12463..13002
                     /gene="pyrR"
                     /locus_tag="PLV81_05175"
     CDS             12463..13002
                     /gene="pyrR"
                     /locus_tag="PLV81_05175"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOM09524.1"
                     /translation="MCEEKVLMNAKQIEEAINTISDGIIKEFGDVTNVALVGIQTRGV
                     EIAKRIKKRLEKNSGKEIKMGTLDITFYRDDLATRGMLPAIKETTITFNITKKIIILV
                     DDVLFTGRTIKAALETLMSFGRPQIIRLAILVDRGNRELPIQPDYCGLKIQTKIGDKV
                     KVRLKETDGIEDTVLYCTE"
     gene            13016..13891
                     /locus_tag="PLV81_05180"
     CDS             13016..13891
                     /locus_tag="PLV81_05180"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HOM09525.1"
                     /translation="MKPTFHCIPCHVNHSYRVIQKLNLDHDSALRLMQELTSISIGSY
                     QTPPDCAYELYTTITKITGIHDPFSDIKKQSNMLMLQFLPQLRTLCTAGEPIRQALRI
                     ALAANVIDYGIGDTDAKIDYWEAFNTVMNDIYVPDDFYNEFIKAASSSQKILYIADNA
                     GEIVADRLLMEYLAVEKITCVVRGNPVINDATIEDAQSVGLNTSVTVITTGDRTPGIN
                     LDRCSKNFLHELSQADMVILKGQGNFETMIDAPLDGIVKKGLKMFFIFKVKCLPVAHY
                     IHRRLGDSAFILREI"
     gene            complement(13937..15184)
                     /locus_tag="PLV81_05185"
     CDS             complement(13937..15184)
                     /locus_tag="PLV81_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019492656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HOM09526.1"
                     /translation="MTHHNKVFGLSPEAGRWVYVITGLIICLCLGSVYSYSVFLKPIT
                     ESFGITATQGNLPFMFFLAFFSILMFFSGKIMAKLGAKKLSIFGGIVVGLGWALSYLA
                     VEAKSINLLILTYGIIAGSGVGIVYGCPINIVGQWFPDKKGLATGLVLAGFGGSALIT
                     AKLAGALIPSVGLSTTFLYFGIAFAILIVLLALPFRNPESGWKPAGWAPSAAAASAVD
                     YDSSEMVKTSTFWGLFLAYIIGCLAGLMAIGISKPVGSEIIKIDGETATTLVGIFAIF
                     NALGRPLFGWLTDKITPKGAAILNLLIIITVSIMMLMAKEGDVTLYVVAFCGFWLILG
                     GWLAIAPTTTLAFYGLKNYARNYGVVFFAYGLGAIIGGIISGQAKDVFGSYQVAFWPT
                     LVLGVVGLMIVMAFLKPPVKKAA"
     gene            complement(15292..16293)
                     /locus_tag="PLV81_05190"
     CDS             complement(15292..16293)
                     /locus_tag="PLV81_05190"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HOM09527.1"
                     /translation="MKKIAVLLVCIIAIVAILIGFEMFGKSDTQVTVEEVKQQLPAPE
                     FYKGIYLNSQSGRDINLLKNFISKAKDAGFNAVVIDVHNESNNKFLIPKENIDYVISQ
                     GIYPIARVVMFPDGLKVYPIPDELIKGRLELAKKACEIGFREIQFDYIRFNDYGILKH
                     VPLEQRYKTIEGFLARAKTELQPYNVKVACDVFGRIPLNTNDTIGQRMEGLDQVVDVI
                     CPMAYPSHYTWSKKLMADPYYTVFITGKRAKERTKNAQIVMYIQAFKIRVAMSGLSFQ
                     EYMAKQIQAVHDAGVKGFIWWNAAQQYDEPFAVTRQFYTNFKEQSISKADNSKKEAI"
     gene            16478..16912
                     /locus_tag="PLV81_05195"
     CDS             16478..16912
                     /locus_tag="PLV81_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004578305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HOM09528.1"
                     /translation="MRLWSIHPHYLDPQGIVAVWREGLLAKKVLEGNTKGYRHHPQLI
                     RFANYIKPLEAIQAYLYWIYEEAKRRGYQFSADKIEAKPLVALIPATYGQLQYEFEHL
                     LKKVYIRNRVWYQELSHKKKDIQCNPVFYCIEGDSEPWEKKH"
     gene            16894..18153
                     /locus_tag="PLV81_05200"
     CDS             16894..18153
                     /locus_tag="PLV81_05200"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOM09529.1"
                     /translation="MGEETLKSRIISDNPFRALRVYNFRLFFMGQFISLVGTWIQQLA
                     SSWLMYRLTASSMWLGLLAFSAQLPIFLFTPITGVVADRYNRHHILMITQILLCVQSL
                     VLGLLVVLGYITPVWLLVLNIMQGCFNAFDMPVRQSFVFDMVAEKELLSNAIALNSVV
                     FNSTRLIGPPLAGFLVAQFGEGVCFLINTTTFSGIIVALLFMKNVTYTSLSRAGNFKE
                     NIHEGFSYVKNHYPIRTVLLLLAFISLLGMPYMVLMPVVAVEILHGDSHTLGFLLGTA
                     GFGALIGALFIASRSHTTRFENTIAAGSLLLGCGLVLFSFSQNLYLSLVLMFIIGISL
                     IVQMASSNTFIQSMVEDSMRGRVMSLYTMSFMGMATIGNLFAGIIAHRIGVPLTLLLS
                     GVGCTIVALVFYRNLHVLKKNIVYFIQ"
     gene            18360..18653
                     /locus_tag="PLV81_05205"
     CDS             18360..18653
                     /locus_tag="PLV81_05205"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOM09530.1"
                     /translation="MEKKDIVKYWKETSDRDYETMLHLFQSKDFHWSLFIGHLVIEKL
                     LKAIFVINNDHNIVPPKTHDLLLLAKKAGIETNDEINDMLDFITTFNINCTIS"
     gene            18732..19052
                     /locus_tag="PLV81_05210"
     CDS             18732..19052
                     /locus_tag="PLV81_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOM09531.1"
                     /translation="MAAFNDRESIHAIINKYVNLLEKKYSVKFVYLFGSRVQGNADFD
                     SDIDMAVIADDFTGDPVEDRLSLMRIRRNVDLRIEPVPFLTKDFVPDNPFVNEILEKG
                     VRIR"
     gene            19167..19616
                     /locus_tag="PLV81_05215"
     CDS             19167..19616
                     /locus_tag="PLV81_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009545909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOM09532.1"
                     /translation="MTILTTIESDLKDAIKNKNELLSSTLRMLKSDIMYEKTKGTEEL
                     TDEKIIEVLTRSAKRRKEAITEYEKAGRDDLASKEKQELEIIMKYLPQQMNEAEIAAY
                     VDNVIASMGTVTKKDMGKVMGQVMKDLKGKADGQVVKQIVQNKLENL"
     gene            19640..21412
                     /gene="dnaG"
                     /locus_tag="PLV81_05220"
     CDS             19640..21412
                     /gene="dnaG"
                     /locus_tag="PLV81_05220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOM09533.1"
                     /translation="MAIPRDTIEHIRQKAAIEDIIKRYVPTLTKKGKNFIGLCPFHKE
                     KTPSFTVSPDKQMFYCFGCHEGGNVFTFISKVEHVDFVESVKIVGELVGIPVRDDVTY
                     RDDEYSRLLKINAIIGQSYRKALLQPANRHALDYLLNRGVTRQSIDEFELGYAPDEWR
                     YATDIVKNMNFSLDVAVKTGNINKVVKNGTTHYYDRFRNRVIFPIFDQKNEIIGFGGR
                     IIAQGEPKYLNSPESPVFQKRNILYGLNKSRQHIVELGRAIVVEGYLDVIGVYQAGIH
                     NVVAPLGTALTLEQLQLLSRLCNEVIIVFDADSAGIKASLRSLDVAQDLNIDIRIAQL
                     PQLDPFDFVAKFGVREFMAIIDRALQPVDFRIERIIAENKGKQIDILKQLFAVLRDIP
                     SEVQRQQYFKKLATMFQVTEQAIRTDYERFAKGQKTNGGAIVTVNNPLDFYDKSQREL
                     VLLLCNYPELVEHAVVDCSLTQFTDTVAKNIYQAILDLYEKNEPVSLDKMFDFFPEGQ
                     EAQLLSQGFSKQFAFEDPKVAYSEIILNMRLYTIDKKINEFAEKIKKNQQDNIQYYMT
                     ELEVLRREREKLASYIYNKGSAKI"
     gene            21452..23215
                     /gene="rpoD"
                     /locus_tag="PLV81_05225"
     CDS             21452..23215
                     /gene="rpoD"
                     /locus_tag="PLV81_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOM09534.1"
                     /translation="MAKGDVLLESIPEVKKLVDLGKTNGEITYDELNDILPDKVLNSD
                     KIDDIFILLNQLGIDVVEEPTRRAEAVLPKKEKSHSKKASAQTSETAVSDDPIRLYLK
                     EIGKVSLLSGDEEVKLAKRIEEGEILIENAVLDSTVLLNELIKNHSKVKSGKIKLTDV
                     LRINKLYYFSSFDMHDLEKRYENNMSIIIAEDKKIMQYHNKLRKLAEDSKKALELKEK
                     IAEARKIIKDALVNLQVHENEINKTAQRILSMVTRIKETRHFFKSIENHFNKSVKELK
                     QYGRKLEKNEDVKKILKELKVKTKDEILEIVKDIRNNERKIRRIEQEAGATCDEIMEW
                     GTQIEIGHRKISLAKKELINANLRLVVSIAKKYANRGMHFFDLIQEGNIGLIKAVDKF
                     EYRKGYKFSTYATWWIRQAITRAISDQARTIRIPVHMIEQINKVMRETRLFQQEYGRE
                     PSPEELADRLGWPVSKVKGVKNVAREPISLETPIGEEEDSLLGDFIEDKEVDSPVNTA
                     AYRLLSEQINAVLSTLPAREQKVIRMRFGLDDGYSHTLEEVGYVFKVTRERIRQIEAK
                     ALRRLRHPTRARKLKDYLDYM"
     gene            23425..25782
                     /locus_tag="PLV81_05230"
     CDS             23425..25782
                     /locus_tag="PLV81_05230"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOM09535.1"
                     /translation="MQHTADNAIMKAVTVIGAGSWGTAVANVIAHNHPEIVVRMWAYE
                     KDVARSVNSQHINTLYLPDVQLANNIVAYTDLKESVRGTDAVIVATPSKVVYDTCTRI
                     RRYLDSKVYVGYLSKGFCKVGSGIYTMSQTIELALPFLMDKVCALYGPSHAEEVCREY
                     HTALHVASKSKSSINFFMNLLQNEYLECIPSDDIIGVDCGSTLKNPAAIAAGIISNLP
                     QCGDNLAGALIAQAHAEMIQLGRCLGAKEQTIMGIAGLGDLVATALSQHSRNRRFGRE
                     IAEQITQKGTTVSFFDKLLLRVKPENVLERMSKRMHYLVEGAYAIEPILELADKYNLT
                     MPVYRSLYDVLLNKRDPWLLIETIKNPAKYAILTRRARIKVKERKKGIERMSGMIFKN
                     IVVEQLVQQLCSESKKLQVLANSREYKELLKQYLPQHKEYSHELSLYNDLNEAQYEKQ
                     LKTIIEFYYNSISDRYVYTFSLLILKLARMFFYLYGLLYRRRITEFFEERIGLSGDIK
                     YLKKTVMTANPVYFCNAKDQADSLFVVLALIKFISIPLPRFYVDSRLMKNKLLQFLFR
                     LCGGYIVHTTRCASILYRETLLQYMLSCVEHGIPVLYSNSMDNESQDELVIQDMVIEG
                     LCALLQKTTEEIAVIPVGIGHKYYNPVTHPVSFLTIFRNVTKVHVSRPITLSHFSASP
                     TLAEDTKMMLTLKQRHDIPIYPHYFVAHVLHTAGGSAHVEAIKAEIDSILKLRNLKHL
                     YSVEDIMREGVDFLISNNYIARSGETVVVLDDKQEAITYFAGYIV"
     gene            complement(26001..26510)
                     /locus_tag="PLV81_05235"
     CDS             complement(26001..26510)
                     /locus_tag="PLV81_05235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HOM09536.1"
                     /translation="MKKTQIVIIGSADDTEHYDTAYAIGSFVAKNGWVLITGGRTGVM
                     EAASKGAHDHGGIVVGILPFDRFDGANQYCDIVIPTGIGFARNSMNVLSADVVISIGG
                     KAGTLTELAYAWHYGKPIISCVFTGGWSKRIIDQPIDDRNNGSVIYPAYSLDDVFKIL
                     VTIVNTSRE"
     gene            complement(26527..27426)
                     /locus_tag="PLV81_05240"
     CDS             complement(26527..27426)
                     /locus_tag="PLV81_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09537.1"
                     /translation="MKNDLTAILKDIQQTIASFSEGLISFDEALKKYHTYKEKTLEIK
                     SQPQYENYILNVLATHNAENFSLFSAIVAYTARNNQTSVLLYHGPDVFVQPLYSLCNN
                     TFFSSPPRFVLDYTVQQNVIQNFSLHSSSLFHSEKLCIAFIAISSSSFFSRESFLRYA
                     AHIKVFTSLLGISDIISLDVPNTTLKYIHQVCSKLLTRGSCSLYGIYIISPFSTLSHY
                     GLHSLNEVSALIKNTLIENFGQYSFALSMNLYLCINTNTQADIKSLTFNYRNISIPSK
                     IYHTVIETEADIIPAITRILHFH"
     gene            complement(27600..28757)
                     /locus_tag="PLV81_05245"
     CDS             complement(27600..28757)
                     /locus_tag="PLV81_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOM09538.1"
                     /translation="MDFNLSEEQQMVKDMCRKFAENELAPKAEHYDRTHEFPWEHVKK
                     LGEMGMMGVVYPEEYGGSGMDYLTYAIAVEEISRGCASTGVIVSAHNSLCLSPIYYFG
                     TEQQKKKYLPKLCSGEWIGCFGITEPNAGSDAAGTKTTAEFKNGKWVLNGTKNFITNG
                     GVASVAVVMAVTEKGVGHKGLSMFIVEKGTPGFSVGKTEDKLGICASSTTELVFENCE
                     IPEENLLGKKGDGFKIAMHTLDGGRVGIAAQALGIAQAAMDAAVKYSLERVQFNQPIA
                     RQQAIQWMIADMATEIQAARLLTYQAAMLIGSGPDRRRYSKNAAMAKLFASEASHRAT
                     HKALQIFGGYGYIKDYPAERFYRDSRITEIYEGTSEIQRLVIASNVLSEYQ"
     gene            28951..30012
                     /locus_tag="PLV81_05250"
     CDS             28951..30012
                     /locus_tag="PLV81_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09539.1"
                     /translation="MVKKILMAIACSMLISCGYFRVTDLSANRVCAIPSGAALGAVSI
                     NYNESGLLDMSFGVLLADDKVYVKDNIQKKVLVFDRDGKCLIAIGNVSATAEGKDIKK
                     TQVKFDIIGTMVADSDGNLYVQNRLASVQRIDAAGTQIDMSPSFILVFDEEGKLQYTI
                     GQTGMPTVPFYYIEHLEIDKKNRLFVIARTYDIWSVYRFDKRQRDYYMTFSRNYFVDT
                     VDATTSYTGKIENIRILHSGDSFLLSVAYYNNTRFKYRKLYQFDIKESKPVELLKISE
                     PKNELFTVAQNKYIYVWNVEETNEVKFMLYNFDGTIIGNVRCKFPGQALFSDIDITSE
                     GKLYSYHAYSTGLEVYEWK"
     gene            30016..31584
                     /locus_tag="PLV81_05255"
     CDS             30016..31584
                     /locus_tag="PLV81_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOM09540.1"
                     /translation="MKVVTAQQMQEIDRITIQQYGIPGEVLMALAGKSVADYVLHTVK
                     AHNVAVFCGVGNNGGDGFVAAYLLSQYLPVDVFILGNTDKFTPSSRIYYDVCQHTSVR
                     CCTISPETLATINLNSYDCIIDALTGTGFSGKPKGLLEQVINVINQSGEKVIAIDMPS
                     GLPADGAVEFEPIVQAHTTITMGLPKINAVTYPGKYYAGNVIVADIGFPYSLCTSGDI
                     CRQLIDEKFFTGNYRPNIHYDAHKGANGHLLCIGGFDGMEGAILLCAKAAFATGIGLV
                     TIVTTHQARAIIAGKIPEAITRALNATASEPFTKDMYGTIEFTNVYKDMLSQLESIIQ
                     LARPTACIIGPGLGRSVSSAATIAACLKIAHTMQLPVLIDGDGLYCLAQLPEAKLPLH
                     CVITPHFKEAERLTGMAVSDIKKNRLRSAEDMAQKLQCVTVLKGPASIVTSGTTTYIN
                     TTGNPALATGGSGDVLSGIIGALLSKGYDPLIAAALGVYIHGKAADIYCQGSGSSMMQ
                     AGDIVRFVRNALNC"
     gene            complement(31624..31836)
                     /locus_tag="PLV81_05260"
     CDS             complement(31624..31836)
                     /locus_tag="PLV81_05260"
                     /inference="COORDINATES: protein motif:HMM:NF039402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HOM09541.1"
                     /translation="MAVTSVRFNAKEEKILKLLQKHYNCDTSSLIKKSLWELYEDIKD
                     KEIIEDFEKKEKSKKARFVSIDNIIQ"
     gene            complement(31827..32096)
                     /locus_tag="PLV81_05265"
     CDS             complement(31827..32096)
                     /locus_tag="PLV81_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09542.1"
                     /translation="MILYEKSVQKAIQKGEIPLRTIQLFHNAFKALDLTKDFSLFDIK
                     KLKGNYKRDYYRLRKGKYRAIFYMENNDFYVIYIGKREEVYDTWQ"
     gene            complement(32144..33055)
                     /locus_tag="PLV81_05270"
     CDS             complement(32144..33055)
                     /locus_tag="PLV81_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOM09543.1"
                     /translation="METVIVENITKRFGDFTAVDRVSFSVAQGEIFGLLGPNGAGKST
                     TIKMLCGLLQPDEGTGSVGGLDIVHQQWDIKKIIGYMSQKFSLYEDLTIEENLEFYGT
                     VYGLSANLLQQRMNFIYELVNIKDYRHSLVKNLPLGIKQRLALGSALIHDPQILFLDE
                     PTSGVDPLMRRIFWDEIYTLAKEGKTVFVTTHYMDEAEHCNRIALIIAGKIIALDTPQ
                     GLKEKLPYTIVKIVTDSYLKAYNVLSKENFVIEVSLFGTDLHVVLEKQYKHFTIIKKL
                     LQSHNIHDVTIEPILPTLEDVFVMSAR"
     gene            complement(33058..34209)
                     /locus_tag="PLV81_05275"
     CDS             complement(33058..34209)
                     /locus_tag="PLV81_05275"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOM09544.1"
                     /translation="MKLSLHNIRIMIKKEFTLVMRDPKLRAIMIVPPILMSLVFGYAV
                     NTDVTNVSFAVFDRDRTSLSREFLQQFIASPYFEMYAYLDSPKQSLRLIEKGDIDFYL
                     EIPNNFGKNVKIGKPVALQVILDGTDSSRASVIQSYINALTLSYVQERFISTIKMKVM
                     QRLQSGISSGTIELIPRYWFNQDLKSINFYLPGVIAMLISLITIMLTAMSIVKEREAG
                     TMEQIKVSPLKPNEYIIGKLIPFAIIAFVDIIVITAIAIFWFGIPFRGSFVFLLLCGA
                     IYIISTLAIGLFISTVSSTQQQAMLSSFMFFVPAILFSGVIFPIYSMPVIFQLITYIN
                     PMRYFVEIVRSVFLKGTGITCLWQHMLILLIMGITILRISVWQFGKRTG"
     gene            complement(34215..35354)
                     /locus_tag="PLV81_05280"
     CDS             complement(34215..35354)
                     /locus_tag="PLV81_05280"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOM09545.1"
                     /translation="MKTLIRILAIAQKEWIHIYRDIRSLILALITPAFLVLIFGYALN
                     MDVKNVAFIIHDQDNTPSSRQFAEIFMHSEYLSFKGYVSRYHDIDTAIDRGKAVAALV
                     IPYNFTENVKSGQSVRLQLIVDASDSMSATIASAYFNAIVSQYNIAWMQEHLNRAGFG
                     SIQLPVHIESRVWYNEELQSKNFVVPGIIVIVLAIISALITSLAISREWERGTMESLM
                     TTPVRSWEVITGKLIPYIFIGIFDVVITYLVGYFVFDIPVRGSFAELLLVSILFLIGT
                     CTFGLVISSATRIQVLSIQVAMIATYLPTILLSGFIFPIKNMPIILQAITYIIPARYM
                     IALIKGIALKGLAASLLTTQIVFMTVFACIMIIIGIKKINVRYAG"
     gene            complement(35356..36270)
                     /locus_tag="PLV81_05285"
     CDS             complement(35356..36270)
                     /locus_tag="PLV81_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOM09546.1"
                     /translation="MIDVSKVTKSFGSIKAVNNVSLTLHKGEIIGIVGPDGAGKSTLL
                     RMAAGILQPDSGNLYFDGRPYDDDIYYVKERIGYMPQRFGLYEDLTVLENIIFFGKIF
                     GMRQKEIQKILPVLWRFSNLEPFKDRLAGKLSGGMKQKLGLACCLVHRPLVLLLDEPT
                     NGVDPVSRREFWRILYDLCVEGVTIMVSTAYLDEAERCNRVALMYNGSFFTVDTPTKV
                     KAMIGKPLIEIQSKSPVETEKMLKQNLTYAHAVRSGNAVRVFVDNVHRAKKEISMLCT
                     RHHIGVISIREVIPSLEDTFVEAIAKAQ"
     gene            complement(36267..37067)
                     /locus_tag="PLV81_05290"
     CDS             complement(36267..37067)
                     /locus_tag="PLV81_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOM09547.1"
                     /translation="MKNKRIIPVIIVLVIVVGVTLYFEFVRYIGTNGKRIEGSGVIEV
                     TEIEVASKIAGRIVSIPFDEGEEVKQGDLIAKLAYDELSAQKLSALANLNNAEKNLKR
                     ARELFTTGSISQREFDNAVTAYNVAKGNYDYVMATIEQAVLYAPVSGVVLSRNLEVGE
                     LAFPGTSIMTLADLTKAWIKIYVNEKHIGLVKHGQKALVFVDSYPDKTFTGKVVAIAQ
                     KAEFTPKTIQTKEERVKLVFAVKIAIDNSGKELKPGMPADAVIETGEQ"
     gene            complement(37057..38454)
                     /locus_tag="PLV81_05295"
     CDS             complement(37057..38454)
                     /locus_tag="PLV81_05295"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOM09548.1"
                     /translation="MKAKGFVIQVLSPIIFLLFISYAHAEQPLSLIDAIDIALKNSPE
                     YIMAQHRLQQADEKIAQAWGMLYPALESEASAMRQYAENGFMSLSDGQYDLKFVQVRF
                     GINPATFYNSLSQSYNNYKLAKEEVKKVKSSIEYNVIKSYFDVITANEIIRLRENSIA
                     VLQANVADVNQMFNTGTIPKFELLQAQVQLQSQEPLLIEARNNFSTALDLFNYYLGAK
                     NGTYTVIFEGFDSSIKEPHGYDIVDTLVEKALMHRPEVIQLTLTRDIVGDSIDAAQSV
                     YLWPTFSVAGYYGKSYLMPDAPQIQLPFPGQSIDLSAITGTRQWQTTWQVRVAATYRW
                     GALAPVDTVQGVEREQKEKLKEINQQILQLKQAIAIAVRADYGRLMTAYQSMVSQRKN
                     IETATEGLRVAKESYKAGVIKNADLLNAELQLTSARMGYIKAINDFWYAVAQLKKDTG
                     FDCTDLIFAEVHNEK"
     gene            complement(38451..39059)
                     /locus_tag="PLV81_05300"
     CDS             complement(38451..39059)
                     /locus_tag="PLV81_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOM09549.1"
                     /translation="MASSKDKILNAAIDEFSRYGLDGARIDRIAKRARVNKAMIYYHF
                     PGKEALYASILQELAQTIVDTISTSIAKVQETGNPLIIIEAYASHISNLDRRYFMIIM
                     RELASGGKFIKNIIAPTIIEPVTERIGNLFEAVKSKRINPKLHPQYTMISVAGAVIFY
                     NLMRQILQGTSLHALYFSDNHVHEYIANLLQLMSHGIIKEDI"
     gene            39747..41030
                     /locus_tag="PLV81_05305"
     CDS             39747..41030
                     /locus_tag="PLV81_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09550.1"
                     /translation="MKLRTLLILCVYFISFFECIVFGNLLAINITEIHGWKSLESDID
                     FLWENQHMYRYFQPNWNYPIAKEQVVERLETLLKKLDKLEAKNSRNIEYYLLKGDIAH
                     YLYNLDKTQYYKKALIMYEKAKTIEPRDYRPYWFIGQHEFLSGHNVKGYESFKTVIDV
                     IPPDKFHWAFWGDYSNCASIGMMPFNFLMAFEFMHKNKAEFENIDQEPFFNTYNDVKK
                     RVTILDPDGEYEVQKIWTKVPSEEIYISTPLGMRIHAKKSWQINPKGLQKRYSVMMMF
                     FDAVKGNNNEDILAGLSIFTYLPKNGETLETHMKKYLDGFKNIRKVTLNNKFNTTHTY
                     ESSMSEIAREQDGMRVFFIFLERDEPAYPCVKIEYPYTPRDTGFFYLKEDATFASRFK
                     GKIFYIIVFETRGSVYSKTYKMFNDFINTSLYFDE"
     gene            41300..>41495
                     /locus_tag="PLV81_05310"
     CDS             41300..>41495
                     /locus_tag="PLV81_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOM09551.1"
                     /translation="MARKLRVQMPGLTYHITSRCIEWRKMLEEEYFKACLVEILRKAK
                     EKYRFKLIAYCIMDNHIHLVI"
BASE COUNT        12867 a         7393 c         8398 g        12637 t
ORIGIN      
        1 atttacaaaa agggatgaaa gtatatttat atagtcgtaa ggaaaattaa aaaaaatagg
       61 agaataaaat taagcaaaga tatttttttt tgttatttaa aaagtcagaa ttacttataa
      121 cattttgtat tgaatactat ttttaatgga gggtaaagtc actatgaaaa aaatagtatt
      181 atatgtagct attgttattt cagcacttat aacctttaat gcttattcac aggagtatct
      241 tgctactggc attatgccat ttacatcttc aagtaaaaat caatctgaaa tcgttgtgtc
      301 acagttatca aagattcttc aagaatatca gtttattcga ttgatagaac gatcgcagat
      361 gcaaaaattg attggcgaaa tagagttctc tcagtcgggg ttggtcaatg aacagtccgc
      421 attgcaacag ggtaaaattt taggcgttaa agttatgatt tttggaacta tcgacaacaa
      481 taatactgta agtgcacgag ctgtgtatac cgaaactggc cgagttattg catcatcaat
      541 gcaatcatcc cagaatattg ccttactggg gaaagaactt gcctatggca ttgaaagctt
      601 tttggctcgt gaaaatttaa agaaaatgcg aaatgacagt cctgatatta aacttgattt
      661 ctggattgaa accaaagatg gtaagcgaat aaacccaaac gatggtttga aaattgggca
      721 atcggtaaag tttaagttca gggcaaataa atcgggatat gttaccattg ttgatattca
      781 gccaacaggt gaggttgtta ttttgtttcc caatcagttt aacatgaata attatattac
      841 tgctgataca gtatactcta ttccttcaga aactgatggg tttgagttaa cggttacaga
      901 accagcaggt gaggatacca ttacgttatt ttttactgaa aaaaaagtgg actggctgga
      961 tatgaaaaag ttacagggtg aaggctttca gagtgtggag cctgctgaac gttttactgt
     1021 aacgagagga ttttcagtaa ctgcaacaca gcttaatact aagcaatggg aaagtgttgt
     1081 tattaatgtt ttagtgaaaa aataagaggt attagtatga aaagtatcaa accggggagg
     1141 atgacttcat ttcagggttt tgtaggttca attattgcta ttgtgtttgg tatattctgg
     1201 ttgtttcttg taacttcttt tatgtttgaa aatgaagata ttccacaagc tatttttttt
     1261 gcgtttgcat tttttggtgt tgtttttatt gttataggca ttgtgaatgc ggttgttgaa
     1321 tataagaatg cgtttagcaa aaaccgttac tcgttattag acgttgtcga tagttctgaa
     1381 gaacaagacc cattccattt taaaaataat actactaaat tagataccat aaactactgt
     1441 ccacactgtg gtacatcaat tcaaaaagag tatacctttt gcccaggttg tgggacgaaa
     1501 ttaaaataat tttagaatag gggtttttat gacacaaaaa ataacagttg aaaagaaagg
     1561 acacatatta ctgatcgggc taaaccgccc tgaaaagcgc aacgcttttg acgtagatat
     1621 gtactgggat ctggcaaaag cgtatggcac ccttgatgct gatgatgaat tgcgctgcgg
     1681 tgttttgttt gcccatggcg aacatttcac cggagggctt gatctggtac aatgggttga
     1741 gccgttttcc aggggggagt atcccgcact tccttcggac agcaaggatc catttggcat
     1801 tgatgaggat aatagggtat caaagccaat tgttgctgca gtgcagggta tatgctttac
     1861 agtgggatgg gagcttatgc ttgcaactga tgtgcgtgtt gctgctacaa atgtgcgttt
     1921 ggctcagatt gaggtaaagc gaggtattta tcctgttggc ggtgcaacgg taaggctttt
     1981 tgaggagata ggctggggca atgccatgcg atatttactc accggtgatg agatgaatgc
     2041 ccaggaagcc taccgccttg ggctggtaca ggaactggtt gagccgggaa agcagttaga
     2101 gcgtgcaatt gaaattgctg aagttatagc caaacaggca ccattggggg tcagagccat
     2161 attacgttcg gcacggctat ctcgtgtgga aggggctaaa gcagcactgg cacgtttgct
     2221 tcctgacctt ttacccataa tgaaaagtga ggatgcacag gaaggagtca aagcttttat
     2281 tgagcgccgc gaagctaact ttacgggtaa gtaattatat catataatgg ggtaatcagg
     2341 agttgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttgag ccaccaaagc
     2461 ggttaacaat ccatgatggc aaaatgtaat ccagcacagt cagtattgcg gcgatagttc
     2521 ctgcaatcag caaaaaggtg aagctgaaag tagcaaaacc gctgaaatta aaaagaataa
     2581 gtgcaaaaaa gctgagtggt gggccaggaa gtattggaaa tactgaaccc agaataccac
     2641 caatagcaca tagtattgcc attattatta aaactaattc catttacatt gttccattag
     2701 atgaggtttg atttataaac agggatgcag gtatattgca tccctgtatc tttatttttt
     2761 accactcgtc actttgcctg tctacatatt tttctcggac tatagctgta atatcgtcaa
     2821 tatagaagta attccaggca ggcaggcgtt ctaaggtgct tggattgtag agtatcttta
     2881 caatttctat atttcttttc tgtgctaaat atttgtcttc ctgtgctatc tcgtcggtaa
     2941 tgggaactga taatttacgc catcctaaaa agtttaattt accaagtaca agacgtttga
     3001 tgttgccatt tgcatcctta actaaaattg aaagctcccc ggaaaaattc tttccatata
     3061 cccacatgtt tatgtatttg caatatttat ctataatcaa aggtttagct ggtataattg
     3121 ttaatactga accactttta gcaaaaacct ttactcccaa ataccgtttt gaattttgca
     3181 caggagcagg gtaatctgtt cgcatggata ctcctgcttt ttcatccttt accacgcgta
     3241 aactaatatt tttatcagta taatctgtcg tttcaaaatc ttctattata acttctttat
     3301 atacctggtc gcttagctgt ttttcctgtt ctaaaacctt ctgcgcttct ttagcttttt
     3361 catcctgtgc ataaagaatg ccaattgaac agcataacag taatgataca catccatata
     3421 taatcttctt catactgtaa atgacctcct tgatgatttt tgcttatttt gcctcattat
     3481 atagatttat caatgtaatg tcaacagaaa atatttcact atttcataaa acacagtttt
     3541 aatcaattct tatttcagca tatacgccaa agtgatccga taaatgtatg ccatcgtaaa
     3601 ttgtgtcaca gcaaatattt gaattaatta ttttatcagg tggtattgat gtattaacaa
     3661 taatataatc tatacgcttt ggctcaagtt catgtatctt gcttacgtgt gtatacggtg
     3721 attcaaattt tttgttggta tctactttat aatatttttg tatattaaga ttttttaatg
     3781 gattccatgt gtacatatct gtattactat ggtgtatatg agtatcacgg aatccctgtt
     3841 gtaaaaaata ttgcatttcc gggctatcag gtgttgcatt gaagtcgccc ataacgatga
     3901 gtggtgcatg ttcaggaact atcgctttgc aatattctgc catgagcttt gcttcatttt
     3961 tgcgccatgt attatctgct tcaagctttt tgatagcctc ctggtattca gtatctgtat
     4021 atccatattt ttgctgtaat tctttcaata acatcctgtt gtgtggtgta tcaaaagggg
     4081 aagcatgcca gtgggtgaca gcaacataca ccggtttggt attgactatc accctgccaa
     4141 tcagtacctg tgtggcatct tcagtatgga atgaaaagta attggtaata attcctccac
     4201 cggaaagttg tttgtgtcca acctgctgta gctgtaatcc tttgcgggca aggatagcat
     4261 caccttcttt taagtttact ggaatgcccc atcttccaat cttaagacct gctacgccaa
     4321 catggtaaat atagtcgtaa ttaatatcgt ttgcaagccg tttaacataa tcgggtaaaa
     4381 agtttgcttc attaatagct attatatcag gattgtatct ggctatttct ttcaggaatg
     4441 cctgataccg tttttcacgt aaatcagggc tttcatactc acccatttta aatgtaccaa
     4501 tataatcaag cccagaccat acattaatgg tcatgcattt taaggtatta gtttttaagc
     4561 cttcaagtgt atgttgtttt ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnagctatcc atagaacaag aaattcatag cctttggccc aggatgacaa taactatcgc
     4741 atttaatgta ataatgaata ataatgattt cttcatttta ataatgtcaa ttacatttca
     4801 gaatagattc ccgataacta tcgcccagaa tattgtaaca aaattgttga ttgaatttca
     4861 aaaagcgata gtttaagtaa atacactaat ttaagttgac taatgtatca ttgcctgtac
     4921 acactataca tgtcatatga acgggttagc ttttaaaata ttgagaggaa gggtggttgg
     4981 ctgataactg ttttacaatg aaacggatat cattacatag attatacatg aaatatggcg
     5041 gattacgaag aatactacat ctgatatcag ttagtgtttt tgttgttttt ttaatgagca
     5101 cgtgcgcttt agcaggtaat aattttgatt ggttctggtt tgtgtatgaa caaggggaat
     5161 attattccag cacatatact gtatataggc cattatacct gaaaaacaac tatcgtaatg
     5221 cttcattcca ggcttcttta atgccagtac tatactggaa atatgccaca ccacggcaac
     5281 aaactgcaaa aggtgtgttt ggtttctatg aatccgtgga ttactggggg ctacatcatt
     5341 acgaaaatga tacaggtatc ttcccatttc ttttgtatgg catgtcaact gatgaaaatg
     5401 acagatattt acatgtgtgg ccattgggcg gcaccattaa aggcaagctt gcaactgatt
     5461 caattaccac atatattttc ccagggatag ctcttttctt cttatatcca acatcatcgt
     5521 attatgttct tgcagtgtat ttagttgctt ctatagtgcc tgtctatgtt cattatgtca
     5581 gtaaggatta tgatgcatat ggcattttgt ggcctctgat acagtggggc aaaagccctg
     5641 ttcgtgatga tatgcgcatc atgcctatat atgctcatag taaaaagaaa gggttttatg
     5701 ataccgcttc atacggcctt ttgtacaatg tgcgcaagga atatattggc aatgacacca
     5761 tgcacactgt tttttttgtt cctttgtatg cacggcggtg gcttgaatca ggcaaggccg
     5821 agagctcttc gttactgtgg cctttctttt catggggtta caatgataag catggtaatc
     5881 ttgaaataaa ttttccatgg ccatttgttc agatacgtga ttgtgatgat ccatttattt
     5941 ataagcgcat atattttcca ttctatggga aatatattga aagaaataaa accacttttt
     6001 ttattacgcc gctttacttt gtgcttgaaa aacagggaac agggtatgaa tctcagtatt
     6061 ccatttatgg gattcttttc tggtatttta aaagggatta tgataatgag caccagcggt
     6121 atggaaagca ctggcgtttt tataagttgt ggccattgtt tcattatgag tacaatgaca
     6181 gaggcgatag ctcatttgca tttttttcca taatgccttt cagggatgaa gaaggttatg
     6241 aacgcctgta tcaaccgttt ttcagtatta ttgagtattc caggcagtct aatggagaca
     6301 ggcgctttgg gctactctta cggacttatt ttcagagctg gaatgataat tattttgcaa
     6361 tgaaaatccc attcattatt tcatatagtg aaaaaaataa tgcaatgaat catatatcat
     6421 tgctgctgtc atttttcagc tatacaaacg atgatactgt acaggaattg tgtattggct
     6481 ggatacctgt atatagaaat gtcaaagata aaaaagccta cgaagccaga atgaaaaaat
     6541 tagcttcagt acggaatgca gaaggtagtc cattaataca ggatttaagc tatttatatg
     6601 taagttcatt gccaggcgca acggtatatt cattacgatt ttaatatatt tgttatttga
     6661 attgttataa caatgtttaa aggcgatagt taatatagat aaggcttttt gggacctgaa
     6721 tatggtagag aaaattaaac atactgtcat taatgttatt cgcgatattg gcgcaaacac
     6781 actgaatatt attgcaatga tgggatattg catgttgctt tttttagaag ttgtgtatta
     6841 ttctaaagca gcgtttgaaa aacgccgtga aattttaaaa cagatgtact atgctggagt
     6901 aaaaaccttt attgtagtat caatagttgc actttttaca ggaatgatac ttgctcttca
     6961 aacagggctt gaattaaaac cttatcaaca gcaggctctg gttggtaaca ttattattgc
     7021 aacgctaaca cgggaaatgg caccatttgt gacagcaatt atcctgattg ccgcagttgg
     7081 ttcaaccatg gctgctgaaa ttggcaccat gacagtttct gaagagattg atgctctgga
     7141 gatgatggca ataagccctg taaaattttt ggttatgcca cgggttattg ctctggccat
     7201 tatgttacct attgctacaa tttatacaaa tctgctgggc agcattggtg gtggtattgt
     7261 tgcatacttt cagcttgatg taagttttga tgtatattat acccacattc ttgattcatt
     7321 gcatttcaag gctacctatg ttgggctttt caaagcgctt gtatttggca tgatggtttc
     7381 taccatcagc tgtgctaatg gactcagggc aaccaatgga gcgttgggtg ttggtaaggc
     7441 aacaagaaat tcagttgtat catctttcct tatggtactt attgtggggt actatattac
     7501 agctcttttt tatggaaaat aaaattctat agccatcgaa atgtatacat tatgattgaa
     7561 tttagaaatg taaccaaaat actcagtggg acaaaggttc tcaataactt gagtttcaaa
     7621 atagaaaaag gtgaaacgtt tgttatcatt gggcgttcag gtacgggtaa aacagtaaca
     7681 ttaaagcata ttgcaggatt gcttgagccc gatagcgggc agattttagt tggtggtgaa
     7741 caaatgaacg gtatcagcaa aagccagcga gatagattgc gggaaaaaat tggatttgtt
     7801 tttcaatcag gtgcgctgat taactggatg actgttgagg aaaatatagc ccttccactc
     7861 attgagcata aattataccc accagatgaa attaaaaaga tagtagagga gaagatggat
     7921 ttattgcaac tcacatcagc ccgggataag atgcctgctg aaataagtgg tgggatgaaa
     7981 aaacgtgcaa gtctggcacg ggttcttgtt cggaatccag aaatcatttt gtacgatgag
     8041 ccaacatctg gactggatcc ggtgatgtca agtttgatca atactataat aaatcaattg
     8101 aaaaaagatt atacaataac acaggttgtt gttactcatg atatggatag tgcttatgca
     8161 gttggtgaca gaattgcgat gctgtatgat gggagaatta ttcaatgtga tgagcctgat
     8221 ggaatacgga acaccgataa ccccattgtg cggcagttta tttcaggttc cttggaaggt
     8281 cctattgatg tgttatgatt ataaaattat ttggagggtg atatgaaaca ggagatagca
     8341 gttgggataa tagttattat agctcttacg gtgttgggct attttactat tataatgagt
     8401 ggtgaaatct ttgaaactca tacatattac ccaatgacag ttgtgtttaa agatgttgaa
     8461 ggtttatcag cggatgataa agtgcgggtt aatggggtat tgtcgggata cgttgatacg
     8521 gtagaattaa tagagaataa tgtaattgta acgttgcggt tatataacca ttttacgctg
     8581 tatgaaaatt atgaaattat ggtacgcaat gaaactgctc ttgctggtaa gtatgtaagc
     8641 atcagtccag gaacaccaat ggatgaaaat ggcaaacaat atgcggttat aaccaccagg
     8701 gataacctta ttgggacatc agtgcctgac ccttttacca tgctttcgcg tcttattgcg
     8761 gataacaggg gaaatgttaa tgccactgtt aagaatttgc gtgatataac tgataaaatt
     8821 aattcaggga aaggaacgct tggcaaaata attaatgaag ataccgttca tgggcaggca
     8881 actgatttaa tcaaacagtt gcaggatact attgaagata cccgtgaaca ggcaccaatc
     8941 acaagcttcc tgcgtgctgc attaacagct ttttaatact attgttacaa tcacgttaaa
     9001 tttttcacta ccaataaaaa aattattgga atttttcttg caccaaactc attgttctga
     9061 agttttaatg gttattttaa taaattttga tacattttga taaaatgtat gtatacacca
     9121 ctacatagaa aggaatgttt gcatgcccag gcgcactgat attaaaaaaa ttttgatagt
     9181 tggatcagga cccattgtta tagggcaggc gtgtgaattt gattattcag gttcacaggc
     9241 ctgtaaagct ttgcgtgaag aaggttatga gatagttctg gtaaattcaa accctgctac
     9301 cataatgacc gaccctgata tggctgacaa aacatatatt gaaccaataa cccatgatat
     9361 tgtagcacgc attattgaaa aggaaagacc tgatgcattg ttacctaccg ttggtgggca
     9421 gactgccctg aatattgctg tccagcttca tgaaagcgga gtattacaac aatacggtgt
     9481 tgaactgatt ggtgcaaaga ttgatgccat aaaaaaagct gaagaccggg atctctttaa
     9541 aaaggctatg cttgatattg ggcttcaggt gccaaattcg gcgctggcat cttcaattga
     9601 tgaagctttg aaaatactgg aaaccattcc acttcccatt attatcaggc cagcgtttac
     9661 tttgggtggt acaggtggca atattgtata taatcaggaa gactttgttg agctggtacg
     9721 aaaagggctt gatgaatcgc ccataagtca ggtattactg gaggaatctg tcattgggtg
     9781 gaaagagttt gagttagagg tcatgcgtga cctcaaagat aatgtggtca ttatatgttc
     9841 cattgaaaat tttgatccta tgggcattca cactggcgat tccataacta tcgcccccca
     9901 gcaaaccctg accgataaag aatatcaaaa tttaagggat atggctatca agataatccg
     9961 tgagattggt gtggaaaccg gcggttcaaa tattcagttt gcggttaatc ccaaagatgg
    10021 cagggtgatg gttattgaga tgaatccccg tgtcagccga agttctgcat tggcgtcaaa
    10081 ggcaacgggc tttcccattg caaagattgc tgcaaagttg gctgtggggc ttacccttga
    10141 tgaaatagca aacgatatta cccgtgaaac gccagcatgc tttgaaccta ccattgatta
    10201 cgttgttacc aagattccac gctgggcatt tgaaaagttt gaaggtgctg acacaacgct
    10261 tggcacccag atgaaatcgg tgggcgaggc aatggcaatt ggtcgtacct tcaaagaatc
    10321 gtttcagaaa gcattgcgtt ctctggagat tgaccgctat gggtttggtt cggatggcaa
    10381 tttaaaactt gatgcacttg ttgatgcaat acctgagcgg caacgaaatg acatcattga
    10441 acagaatttg cgtattccga gggaacaaag agtcttttat ataaagaaag cattggagct
    10501 tgattggagt gttgaaaaaa ttcatgaatt aacatcaata gacccatggt ttttacatca
    10561 actcaaagaa ctggttgaag cagagcgtga atttgttgca caggctaaac agggtttgca
    10621 aaaagaaaac atactcaaaa tgaaacaatt gggttactct gacaggcagc ttgcattttt
    10681 aatccataaa gatgaactca aggcattgta tgaacggggg cttgatgcac agaaggatta
    10741 ttcaaagcga ctgaatgaac ttgaagacag tataaggaag tttagatttg agcaggcgat
    10801 agttcctgga taccggctgg ttgacacctg cggtggtgaa tttgaagcct acacccccta
    10861 ttactattct tcgtatgatg atgaggatga agctaagcac acaacaaaac gcaaggtcat
    10921 gatattgggt ggtggcccaa accgtattgg gcagggcata gagtttgact actgctgctg
    10981 tcatgcttct tttgcattgc gtgaagctgg tatagagtca ataatggtaa attcaaatcc
    11041 tgagacagtt tcaaccgatt atgatacttc ggacagattg tattttgagc cattgacact
    11101 ggaagatata ctccatattt acaacaatga aaaacctgat ggcgttatag tacagtttgg
    11161 aggacaaact cccttgcggc ttgcacgccg acttgaagca aatggtgtta aaattttagg
    11221 gacatcgcct gattccatag acagggccga agacaggcag cgttttgctg aggtagtaaa
    11281 taagcttaaa cttaatcagc ctgaaaatgg cattgccttc acccgcgaag aggcaattgc
    11341 acaagctaca cggattggtt atccggtttt ggttcgtcca tcgtatgtac tgggcggaag
    11401 ggcaatgtcc atcatttatg atgaaaaaag ccttttagaa tatatagtca gtgcagtaga
    11461 gctatcgcct gaacatccta tccttattga taaattttta gaagatgcat tggagataga
    11521 tgttgatgca ctatgtgatg gtactgatgt atttattggt ggcatcatgg agcatattga
    11581 attagcagga atacattcag gcgattctgc atgcagttta ccaccggtga gcgttaaacc
    11641 ttccatgatg caaacaatta ttgaaacaac aactgcactg gcaaaggaac tcaatgttat
    11701 tggactgata aacatacagt ttgcaattaa gaatgatgta ttgtatgttc ttgaggtaaa
    11761 tccccgtgcg tcacgtacgg tgccatttgt atccaaagca accggtgagc cacttgcaaa
    11821 gattgcggta tggattatgc ttggtaaaaa gctaaaggat tttggcttaa cttatatgaa
    11881 aaagataccc tatgttaatg taaaggaagc agtgttgcct ttcaacaaat ttcccggtgt
    11941 tgatacgttg ttatcccctg aaatgaggtc aaccggcgag gtgatgggca tcgctaaaca
    12001 ttttggggaa gcattttata aagctgaact gggagccggc gatagattgc cattgtcagg
    12061 aaccatcttt ttaagtatca accagaggtc aaaagaggaa ctgcttgaag agatgcgtct
    12121 actgcatcaa gaaggcttta agcttattgc cacagaaggt accgcagcct tcctcaatga
    12181 gcatgggatc ccctgtgagc gtgtgtataa agttagcgaa ggccgcccaa atgttgttga
    12241 tattataaaa aacaaacagg ttgatcttat tatcaatact cctacaggaa aacgaccaaa
    12301 agaagatgcc tataccatac gtcaggctgc ggtgcgttat cgggtaccca ttatcactac
    12361 attagcggct gcaaaagctg ctgtacaggg cattatttcc atgaaaaaag ctggacaatt
    12421 taccgtaaaa cctgtacagg agtaccatcg ggaggtgcag tagtgtgtga agaaaaagta
    12481 ttgatgaatg caaagcaaat tgaagaagca attaatacta tcagcgatgg tattatcaaa
    12541 gaatttggcg atgtaacaaa tgtggcgctt gttggaatac aaacccgtgg tgtggagata
    12601 gctaaacgaa taaagaaacg cttagaaaaa aatagcggta aagaaatcaa gatgggtaca
    12661 cttgatatta cattttatcg tgatgattta gcaacacgtg ggatgcttcc ggcaataaaa
    12721 gagacaacaa taacattcaa tattaccaaa aagattatta ttctagttga tgatgtgctt
    12781 tttaccggaa gaacaatcaa agctgctctg gaaactttaa tgtcatttgg aagacctcaa
    12841 ataatacgct tagctatact tgttgaccgt ggtaatcgtg agcttcctat tcagcctgat
    12901 tattgtgggt tgaagataca gacaaaaata ggcgataagg ttaaagtgcg acttaaagaa
    12961 acagatggca ttgaagatac tgtattatat tgcactgaat aaatcaaagt aaaatatgaa
    13021 acccaccttt cattgtatcc cctgccatgt gaatcatagt tatcgtgtta tacaaaagct
    13081 aaatctagac cacgatagtg cattacgatt aatgcaagaa cttacatcca ttagcatagg
    13141 tagttatcaa acgccacctg attgtgcata tgaattatat acaactatca caaaaattac
    13201 aggtattcat gaccccttta gcgatattaa aaaacaaagc aatatgctga tgctacaatt
    13261 tctgcctcaa ctgcgtacgt tatgtactgc tggtgagccc ataaggcagg cattgcgcat
    13321 agcactggct gccaatgtta ttgattatgg cattggtgat acggatgcaa aaattgatta
    13381 ttgggaagca ttcaacacgg tgatgaatga tatatatgta cctgatgact tttataatga
    13441 atttatcaaa gcggcttcat catcacaaaa gattctttat atagccgata atgcagggga
    13501 gatagttgcc gacaggctgc ttatggaata tcttgctgtg gaaaagatta cctgtgtggt
    13561 tcgcggtaat cctgtgataa acgatgcaac aattgaggat gcacaatctg ttggtcttaa
    13621 tacttccgtt acagtgatta caaccggtga ccgtactccg ggtattaatc ttgatcgttg
    13681 cagcaaaaac tttttacatg agctatcgca ggctgatatg gtaattttaa agggacaggg
    13741 aaattttgaa acaatgatag atgctcccct ggatgggatt gtgaaaaaag gcttaaaaat
    13801 gttttttata tttaaagtta aatgcctgcc tgttgcccat tatattcaca ggcgtttagg
    13861 agatagtgct tttatactac gagaaatata aagggcacat tttgtgccct ttatatttta
    13921 gtgatatata tagtgtttaa gctgcttttt ttactggtgg cttaaggaat gccatgacta
    13981 tcatgagtcc cactacacca agaacaaggg ttggccagaa agcaacctga tatgaaccaa
    14041 acacatcttt tgcctgcccg gatatgatgc cacctataat ggcaccaagg ccgtatgcaa
    14101 aaaatactac accataatta cgtgcataat tcttcaaacc ataaaaagca agtgttgttg
    14161 ttggagcaat tgcaagccac ccacctaata tcaaccagaa tccacagaaa gctactacgt
    14221 aaagggttac atcaccttct tttgccatca acatcattat ggacacggtt atgattatca
    14281 gcaagttaag tattgctgca ccttttggtg taatcttatc agtgagccat ccaaaaagtg
    14341 ggcgacccaa tgcgttgaaa attgcaaaaa tcccaacaag ggttgttgca gtctcgccat
    14401 caattttgat tatttcactg cctactggtt tagaaatacc aatagccatc aagcctgcta
    14461 aacagccgat gatatatgct aaaaaaagac cccagaaagt tgatgttttt accatttcag
    14521 atgaatcata atcaactgca cttgcagctg cagctgatgg tgcccatcct gcaggtttcc
    14581 agccggattc aggatttcta aatggaagtg caagtagtac aataagtatt gcaaatgcaa
    14641 taccaaaata aaggaatgta gttgaaaggc caactgaagg tatcagagcc cctgctaatt
    14701 tagcggtgat caacgcagag ccgccaaaac cagccagtac caggcctgta gcaagacctt
    14761 ttttgtcagg gaaccactgc ccaacgatgt taataggaca tccataaaca atcccaacac
    14821 cactacctgc aatgatacca tacgttaata ttaaaaggtt aatgcttttt gcttctacag
    14881 caaggtatga aagcgcccat cctaacccaa caacaatgcc accaaaaata gagagctttt
    14941 ttgcccctaa tttagccatt attttaccgg agaaaaacat aagaattgag aaaaatgcaa
    15001 ggaaaaacat aaagggaaga tttccctgag tggcagtaat accaaagctt tcagtgatag
    15061 gttttaagaa tacactgtat gaatacacac tgcctaaaca caggcatatt atcaagcctg
    15121 taataacata cacccatcga ccggcttccg gtgataggcc aaatactttg ttgtgatgtg
    15181 tcatataacc cgatcctcct gtaaattttt agttacttat aggttttata taaaaaacta
    15241 tggaaagcaa atgtatattc cttttaatag tcaagaatat ttttgttagt tttaaattgc
    15301 ttcctttttg ctgttatccg cttttgatat actctgttct ttgaagttgg tgtaaaactg
    15361 cctggtaacc gcaaaaggtt catcatactg ctgtgcagca ttccaccata taaagccttt
    15421 tacaccagca tcatgaacgg cctgtatttg ttttgccatg tattcctgaa acgataatcc
    15481 tgacattgct acccgtattt taaacgcctg gatgtacata acaatctgag cattctttgt
    15541 acgttctttt gctcttttcc cggtgataaa tactgtgtaa tatggatcag ccattaattt
    15601 tttactccat gtataatggg aaggataagc catggggcag attacatcaa caacctgatc
    15661 aagcccttcc atacgctgac caatggtatc gttggtatta agtggaattc ggccaaatac
    15721 atcgcaggca accttgacat tatacggctg taattcagtt tttgctcggg ctaagaatcc
    15781 ctcaatagtt ttatagcgct gttcaagggg tacatgtttc agtataccat agtcattaaa
    15841 ccgtatataa tcaaactgta tttctctgaa gccaatttca caggcttttt ttgcaagctc
    15901 cagcctccct ttaataagct catcagggat gggatacact tttagcccat cggggaacat
    15961 aaccacgcga gcaatggggt atatgccttg cgaaattaca tagtcaatat tttctttggg
    16021 gattaaaaac ttattattgg attcattgtg tacatcaatg actactgcgt tgaatcctgc
    16081 atcttttgct tttgaaataa aattttttaa aagattgata tcgcgccccg attgtgaatt
    16141 taagtaaata cctttataaa actcaggtgc aggtagttgc tgtttgactt cttcaacagt
    16201 gacctgcgta tcgcttttgc caaacatctc aaagccaata agaatagcta cgatagcgat
    16261 aatgcataca agcagtacgg caatcttttt catagtggtt cctttctgta agttatgatt
    16321 gtggtatatg gtgatactat tgcttaattt gtcgactaaa atatatattc gttgtaccaa
    16381 aaaacagtgc taaaattttt atttacttat aatttagggt tgttatgtaa tacaatgtgt
    16441 agtagtatat tctatcgata gttatataca atatcttatg cggttatggt caatacatcc
    16501 acactattta gaccctcagg gaattgttgc cgtatggcgg gaagggttgc ttgcaaagaa
    16561 ggtacttgaa gggaatacta aagggtacag gcatcatccg caattgatac ggtttgccaa
    16621 ttacattaag ccgcttgaag ctatacaggc atatctttac tggatctatg aagaagcaaa
    16681 aaggcggggc tatcagtttt ctgctgataa gattgaggca aaaccactgg ttgcattaat
    16741 ccctgccacg tatgggcaac tacaatatga atttgagcat ctgctaaaaa aagtgtatat
    16801 acgcaacaga gtctggtatc aggaactatc gcacaaaaaa aaggatatac aatgtaatcc
    16861 ggtgttctac tgtattgaag gtgatagtga accatgggag aagaaacatt aaaatcaaga
    16921 attatatctg ataacccatt cagggcactg agggtatata atttcaggct cttttttatg
    16981 ggtcagttta tttcactggt tgggacatgg attcagcaac tggcctcaag ctggctgatg
    17041 taccgtttaa ctgcatcatc catgtggttg gggttactgg cattttctgc acagcttccc
    17101 atttttttgt tcacgcccat taccggtgtg gtggctgacc gctataacag gcatcatata
    17161 ttgatgatta cccagatcct tttatgtgtg caatcccttg tattaggatt actggtggta
    17221 ctgggatata ttacaccggt gtggcttttg gtgcttaata tcatgcaggg gtgctttaat
    17281 gcctttgata tgccggtgcg acaatcattt gtatttgata tggttgccga gaaggaactt
    17341 ttgagcaacg ccattgccct taattcagta gtatttaaca gtactcgcct tattgggcca
    17401 ccgctggctg gtttcctggt ggcgcagttt ggcgaaggtg tttgcttttt aataaataca
    17461 acaacgtttt cagggattat tgttgcattg ctttttatga aaaatgttac atatacttct
    17521 ttatcccgtg cagggaattt caaggaaaat attcatgaag gcttcagtta cgtgaagaac
    17581 cattacccaa tccgtacagt gctattgctg cttgccttca ttagtttgct tggaatgccc
    17641 tatatggtgc tcatgcctgt tgttgcagtg gaaattttac atggtgattc gcatacattg
    17701 ggttttcttt taggtacggc agggtttggt gcgcttattg gtgcgctgtt tatagcatca
    17761 cgaagccata ccacacgatt tgaaaatacc attgcagccg gttcattgtt gcttggatgc
    17821 ggactggtac tgttttcatt ttcacaaaat ttgtatcttt cactggtcct catgtttatt
    17881 attggtatca gtttaattgt tcagatggca tcatccaata cgtttattca gtcaatggtg
    17941 gaagattcaa tgcgtggcag ggttatgagc ctgtatacca tgtcattcat gggcatggct
    18001 accattggta atttatttgc cggaataata gctcaccgta ttggtgttcc tcttacactg
    18061 cttttgagcg gtgtggggtg tactatcgta gcgctggttt tttatagaaa tttacatgtg
    18121 ctgaagaaaa acattgttta ttttatacaa tagctttaca aaaatatgaa gctatatata
    18181 ctttaataat gaatataatt aattattgga tggtgaatca ttaataatga aaataatgaa
    18241 aatttgaaaa tatatagaat tattcatcat gaactaaata cataattcta actattaaat
    18301 tcttgacgta atcattattt taatttatat ttatttaaaa aactttatat tgtaaaacaa
    18361 tggaaaagaa agatatagtt aaatattgga aagaaacttc agatagggat tatgaaacta
    18421 tgctacatct tttccaatca aaggatttcc attggagcct gtttatagga catcttgtaa
    18481 tagaaaagct tttaaaagca atatttgtta taaataatga tcataacatt gtaccaccaa
    18541 aaacacacga tttacttcta ctagctaaga aagcaggaat tgaaactaat gatgaaatta
    18601 atgatatgtt agactttata acaactttta atatcaattg cacgatatcc tgattataag
    18661 caagaatttt accgaaaatg tactatgaaa tttacacagg atgcaatcaa taaaattaag
    18721 gagttgcggc aatggctgct ttcaatgata gggaatcaat acatgcaata ataaataaat
    18781 atgtaaattt actggaaaaa aaatattctg taaagtttgt atacctattt ggttcgcggg
    18841 tacagggaaa tgctgatttt gatagtgata ttgatatggc agtaattgcc gatgatttta
    18901 caggagatcc tgttgaagat cgtttatccc ttatgcgtat aaggagaaat gttgatctgc
    18961 ggattgaacc agtgcctttt ttaaccaaag attttgttcc tgacaatcct tttgttaatg
    19021 aaattttaga aaaaggtgtg cgtattagat agaattcttt ctaaaacttt tgttgacata
    19081 caaacataat aattttatat gtgttacact ctccgtttta gctttatact tcgcgccata
    19141 aaatccttgt agtatggggt atgtgtatga ctattctcac cacaattgaa tctgacctta
    19201 aagatgcaat taaaaacaaa aatgaactgt taagcagtac tctgcgtatg ctgaagtctg
    19261 acataatgta tgaaaaaaca aaaggcacgg aagaattaac cgatgaaaaa ataattgagg
    19321 tgctaacacg cagtgccaaa aggcgcaaag aagccataac tgaatatgaa aaagcaggca
    19381 gagatgacct ggcttcaaag gaaaagcagg aacttgagat catcatgaaa taccttcctc
    19441 agcagatgaa cgaagctgaa attgctgcat atgttgataa cgttatagct tccatgggaa
    19501 ctgtcactaa aaaagatatg ggcaaggtca tgggacaggt tatgaaagac ctgaaaggca
    19561 aggcagacgg gcaggttgta aagcagattg tacagaataa acttgaaaat ctgtaaattt
    19621 atatatatat tactatcaaa tggctatccc ccgagatacg attgaacata tacggcagaa
    19681 agctgctatt gaagatataa ttaaacgcta tgttcctacc cttaccaaaa aggggaagaa
    19741 ctttataggt ctatgcccat ttcataaaga aaagactcct tcgtttaccg tatcacccga
    19801 taaacagatg ttctattgtt ttggatgtca tgaaggtgga aatgttttca cgtttatatc
    19861 aaaggttgaa catgttgatt ttgttgaaag tgtaaagatt gtaggggaac ttgttggcat
    19921 tcctgtccgt gatgatgtaa catacaggga tgatgaatat agcaggcttt taaaaatcaa
    19981 tgcaatcatt ggccagagct atcgcaaagc attactccaa cctgcaaaca ggcatgcact
    20041 ggattatctg ttaaaccgtg gagtaaccag gcagagcatt gatgaatttg agctgggcta
    20101 tgctcctgac gagtggcgtt atgctactga tattgtaaaa aatatgaatt tttcgcttga
    20161 tgtggcagta aaaacgggca atatcaataa agtagtaaaa aatggtacca ctcattacta
    20221 cgacagattc cgaaacaggg ttatattccc tatttttgac caaaaaaatg aaattattgg
    20281 ctttggtggt agaataattg cacagggcga gcccaaatac ctgaattctc cggaatcacc
    20341 tgtttttcaa aagcggaata ttctttacgg actcaataaa tcacggcaac acatcgttga
    20401 acttggcagg gcaatagttg ttgaaggtta cctggatgtt attggcgtgt atcaggcggg
    20461 tatccacaac gttgttgctc cattaggaac cgcattaaca ctggaacaat tgcagctttt
    20521 atcacgttta tgtaatgagg taattattgt ttttgatgcc gactctgcag ggattaaggc
    20581 gtcactgcgg tcacttgatg ttgcacaaga tttaaatatt gatattcgta ttgcacaatt
    20641 accccagctt gacccgtttg attttgtagc aaagtttggc gttcgggagt ttatggcaat
    20701 cattgacaga gcattacaac ccgttgattt cagaattgag cgtataatag cggaaaataa
    20761 gggcaaacag attgatatat taaagcaact ttttgcagta ttgcgcgata ttccttctga
    20821 agtgcagcgg caacagtatt ttaaaaagct ggctaccatg tttcaggtta cagagcaagc
    20881 tatccgcacc gattatgaac gttttgcaaa agggcaaaaa acaaacgggg gggcgatagt
    20941 aactgttaat aatcctctgg atttttatga taaaagccag cgtgaactgg tacttttgct
    21001 gtgcaattac cctgaactgg ttgaacatgc agttgtggat tgctctctga cacagtttac
    21061 cgatacggtg gcaaaaaata tttaccaggc aatacttgac ctgtatgaaa aaaatgaacc
    21121 tgtttcactg gataaaatgt ttgatttttt ccctgaaggt caggaagcac aattactttc
    21181 tcagggcttt tcaaagcagt ttgcatttga agaccctaaa gttgcctatt cagaaataat
    21241 attaaatatg cggctgtata ctatcgacaa aaaaattaac gaatttgcag aaaaaattaa
    21301 gaagaatcag caggataata tacaatatta tatgaccgaa ttagaagtat tgcgccgcga
    21361 aagagaaaaa ttagcttcat atatctataa caaaggcagt gcaaaaattt aagatcacat
    21421 taaattttta atataaaggg tagagtttat tatggcaaag ggtgatgttc ttttagaaag
    21481 tattccagaa gttaaaaaac ttgtggatct tggaaaaaca aatggtgaga ttacctatga
    21541 tgagcttaac gatattttgc ccgataaggt attaaattct gataaaattg atgatatttt
    21601 cattcttctc aatcagctgg gcattgatgt tgttgaagaa cccactcgcc gtgctgaagc
    21661 agtattgcca aaaaaagaaa aatcccattc aaaaaaagca tcagctcaga catcagaaac
    21721 cgctgtttcc gatgacccaa tccgcctgta cctaaaggag attggcaaag tatccctgct
    21781 ttccggtgat gaagaggtga aactggcaaa acgtatagaa gaaggtgaaa tacttattga
    21841 gaatgctgtg ctggattcta ctgtgcttct caatgagctt atcaagaatc attcaaaggt
    21901 taaaagtggc aaaattaagc tgactgatgt tttgcggata aataaactct actatttttc
    21961 ttcttttgac atgcatgatt tagaaaagcg ctatgaaaac aacatgagta tcatcatagc
    22021 tgaagacaaa aaaataatgc aataccataa taagctacgc aaattagctg aagattctaa
    22081 aaaagcactg gaattaaaag aaaaaatagc agaagcccgc aaaattatca aggatgcact
    22141 ggtaaacctg caggtacatg aaaatgaaat aaataaaaca gcacagcgta ttctatcgat
    22201 ggttaccaga ataaaggaaa cgcggcattt ttttaaaagc attgaaaatc attttaacaa
    22261 atcagtaaaa gagttaaagc aatatggtcg taagcttgaa aaaaatgaag atgttaaaaa
    22321 aatattaaaa gaactaaaag tcaagaccaa agatgaaatt ctggagatag ttaaagatat
    22381 cagaaataat gagcgcaaga ttcgccgcat agagcaggaa gcaggtgcta cctgtgatga
    22441 aatcatggaa tggggtacac agattgaaat aggccaccgc aagatttcac tggcaaaaaa
    22501 agagctcatt aatgcaaacc tcaggcttgt agtatcaatt gcaaagaaat atgccaacag
    22561 aggtatgcat ttctttgatc tgattcagga aggtaatatt ggcttaataa aagcagtaga
    22621 taagtttgag tatcgcaagg ggtataaatt ttccacctat gccacatggt ggattcgaca
    22681 ggcaataacc agagcaattt ctgaccaggc acgtaccata cgtatacctg ttcatatgat
    22741 tgagcagata aataaggtaa tgcgcgaaac acgcctcttc cagcaggaat atggccgtga
    22801 gccaagccct gaagaattag ccgacaggtt aggctggcca gtaagtaaag ttaaaggtgt
    22861 gaagaatgtt gcccgtgagc ccatatcgct tgaaacccct ataggcgaag aggaagattc
    22921 gttattgggt gattttattg aggacaaaga agttgattca ccggtgaata ctgcagcata
    22981 ccggcttctt tctgagcaga taaatgcagt gctgagcaca ttgccagcac gtgaacagaa
    23041 ggttatccgc atgcggtttg gtctggatga tggatactct catacactgg aagaagttgg
    23101 gtatgtgttt aaggtaaccc gtgagcgtat acgacagata gaagccaaag cattgcgtcg
    23161 gttgcgccat cccacccgtg cacgaaaatt gaaagattat cttgactata tgtagccata
    23221 cttctataat agcattaagg ggctgtccca aaagaggtcg ttgagtaaat tttgcaaaca
    23281 ttatatcgta atactcgctc atttcgatac aattttgcca caaaatctgg caaaattact
    23341 caatgagcgt tgcgaaattt gtatcgaaac ggcttttggg acatcccctt tttgttattt
    23401 taatctgtta ataccgggta tactatgcaa cacaccgcag ataatgccat aatgaaagct
    23461 gtaaccgtca ttggtgcagg ttcatggggt accgctgttg ccaatgtaat agcccataac
    23521 catcctgaaa ttgttgtgcg aatgtgggcc tatgaaaagg atgttgcccg ttctgtaaac
    23581 tctcagcata tcaatacact atacctgcct gatgtgcaac tggcaaacaa tattgttgca
    23641 tatactgact taaaggagtc ggtcagagga actgatgcag taatagttgc taccccttca
    23701 aaagttgtgt atgatacctg tacacgaatc aggcgctatc ttgattcaaa ggtatatgtt
    23761 ggctatttgt ctaaagggtt ttgcaaagtt ggttcaggaa tttataccat gtcacagaca
    23821 atagagcttg cactaccttt tttaatggat aaggtgtgtg ctctatacgg cccaagccat
    23881 gctgaagagg tgtgcagaga atatcatacg gcattgcatg tagccagcaa atcaaagtct
    23941 tcaattaatt ttttcatgaa tctgctacag aatgaatatc tggaatgcat accttcagat
    24001 gatattattg gtgttgattg tggaagtacc ttgaaaaatc ctgcagcaat tgcagcaggc
    24061 attataagta acctccctca gtgtggggat aaccttgcag gagcattaat agcacaggct
    24121 catgcagaga tgatacaact gggacgctgc ctgggtgcaa aggaacaaac tatcatgggt
    24181 atagctggtt tgggtgacct tgttgcaaca gcgttaagtc agcatagccg taacaggcgc
    24241 tttggcagag aaatagcaga gcaaataacc cagaaaggaa caacagtaag tttctttgac
    24301 aagttgttat tacgagtaaa acctgaaaat gttctggaaa gaatgagtaa acgcatgcat
    24361 tacctggttg agggggcgta tgcaattgag ccaattctgg aactggctga taaatataac
    24421 cttaccatgc ctgtctaccg ctccctgtat gatgtgcttc tgaataaacg cgacccatgg
    24481 cttctcattg aaacaataaa aaatcctgca aaatatgcaa tactgacgcg ccgcgcacgg
    24541 ataaaggtta aggagcgcaa aaaaggcatt gagcgcatga gtggtatgat atttaaaaat
    24601 attgtggttg agcagcttgt ccagcaatta tgcagtgaaa gtaaaaaact tcaggtgctt
    24661 gcaaatagca gggaatacaa agaattgcta aagcaatacc tgccccagca taaagagtat
    24721 tcccatgagc tatcgcttta taatgatctt aatgaagccc agtatgaaaa acaactgaaa
    24781 acaattattg agttttatta taatagtatt agcgatagat atgtgtatac gttttctttg
    24841 ctgatactga aactggcaag aatgtttttt tatctttatg ggttgctgta tcgccgaaga
    24901 attacagagt tctttgaaga aagaataggg ctgagcggtg acattaagta tttaaaaaag
    24961 actgtgatga ctgcaaatcc ggtgtatttt tgcaatgcta aagaccaggc agattctctg
    25021 tttgtggttc ttgcacttat taaatttata tccatccccc tgccgcgatt ttatgtggat
    25081 agcaggctta tgaaaaataa attgttgcaa tttttattca ggctttgtgg gggatatatt
    25141 gtccatacta cacgctgtgc ttccattcta tacagggaaa cactgttgca gtatatgtta
    25201 tcatgtgttg aacatggcat accggtatta tattctaatt ccatggataa tgaatcacag
    25261 gatgagctgg taattcagga tatggtcatt gaaggcctgt gtgcactgtt gcagaaaaca
    25321 acagaggaaa tagcggtaat acctgttgga attggtcata aatattataa cccggtaact
    25381 catccggtat cattccttac aatattcagg aatgtaacga aggtacatgt ttcccggcca
    25441 attactctat cgcatttttc tgcaagcccc acattggctg aagatactaa aatgatgtta
    25501 acgctgaaac agcggcatga tatacccatt tacccgcatt attttgtggc ccatgtgtta
    25561 catactgctg gtggaagtgc acatgttgaa gccattaaag ctgaaattga tagtatactg
    25621 aaactacgta atttaaaaca tctatattct gttgaagata taatgcgtga aggtgtggat
    25681 ttccttattt caaataatta tattgcacgc agtggggaaa cagtagttgt tttggatgat
    25741 aaacaagaag caattacata ttttgccggg tatattgtgt aatgtatttt acaaatcttg
    25801 tttttaaagc atatacgttt taagataatt tattcacata atagtcctga agtattataa
    25861 agcaatatag tagcattcaa ttaaaaacgg tcataagtgg catgattgga aaaatttgta
    25921 tcatgtacat aaacacagtt aggtgtaaca gtattgtaga agaacatagt accttaccat
    25981 taaactccgt taaggtgata ttattccctg gatgtattaa ctatggtaac taaaatttta
    26041 aaaacatcat ctaatgagta tgcaggatat ataacactgc cattatttct gtcgtcaatt
    26101 ggttggtcaa taatgcgttt actccagcca ccggtaaaca cacagcttat aataggtttg
    26161 ccataatgcc atgcgtatgc cagttcggta agtgttcctg cttttcctcc tatggaaatg
    26221 acaacatctg ctgaaagcac attcatgctg tttcgtgcaa aacctatacc agttggaata
    26281 acaatatcac aatattggtt ggcgccatca aacctgtcaa aaggcaatat gccaaccaca
    26341 ataccaccgt gatcatgtgc accttttgaa gcagcttcca taacgccagt tctaccacca
    26401 gtaatgagaa cccatccgtt ttttgcaaca aatgagccta ttgcatacgc agtgtcgtaa
    26461 tgctctgtat catcagctga gcctataatg acaatttgag tttttttcat ggttactatc
    26521 gcctgactaa tggaagtgta atatacgggt aattgcgggt ataatatccg cttctgtttc
    26581 aataacggtg tggtaaatct ttgatggtat tgaaatattc ctgtagttaa aagttaatga
    26641 tttgatatct gcctgagtgt ttgtattgat acaaagatac aggttcattg aaagtgcaaa
    26701 actgtattgc ccaaagtttt ctattaaggt atttttaatt aatgcggaaa cttcattgag
    26761 tgagtgcagg ccataatggc ttaatgtact gaaaggtgat attatatata tcccatataa
    26821 tgaacatgac cctctggtca ataattttga gcatacctga tgaatatatt ttaatgttgt
    26881 atttggaaca tccagagaaa taatatctga aatacccaga aggcttgtaa agactttgat
    26941 atgtgcagca tagcgcaaaa atgattctcg tgagaagaat gaggatgaag aaatagcaat
    27001 aaatgcaatg caaagttttt ccgaatgaaa cagcgatgag ctgtgcagtg aaaaattttg
    27061 aattacattt tgctgtacgg tgtagtcaag tacaaagcgt ggtggggatg aaaaaaaagt
    27121 attgttgcaa agtgaataaa gcggctgcac aaaaacatca ggaccatggt acagtaaaac
    27181 ggaagtctgg ttattccgtg cagtatatgc aacaattgct gaaaatagtg aaaaattttc
    27241 agcattatgg gtagctaaaa cattaaggat ataattttca tactggggtt gggattttat
    27301 ttcaagagtc ttttctttgt atgtatgata ttttttaagt gcttcatcaa atgatataag
    27361 cccctctgaa aatgaagcaa ttgtttgctg tatgtctttc aagatagctg ttaaatcatt
    27421 tttcatactt tttttatggg ggctgtccca aaagagatcg ttgagtaaat tttgcagaca
    27481 ttatatcgta atactcactc atttcgatac aattttgcca cataatctgg caaaattact
    27541 caatgagcgt tgcgaaattt gtatcgaaac gacttttggt acagccccag tatttacatt
    27601 tattgatatt cacttaatac atttgatgca ataacaagcc gctgtatttc agatgtccct
    27661 tcatatattt cggtaattcg tgaatcacgg taaaatcttt ctgcagggta gtctttaata
    27721 tacccatatc ctccaaaaat ctgtaatgcc ttatgagttg ccctgtgtga agcctccgaa
    27781 gcaaaaagtt ttgccatggc tgcattcttt gaatatcgcc tgcggtcggg gccgctgcca
    27841 ataagcattg cagcctgata ggtcaaaagc cgtgcggcct gaatttcagt agccatgtca
    27901 gcaatcatcc attgaattgc ctgctgacgt gctattggct ggttaaactg tactctttca
    27961 agtgagtatt taaccgcagc atccatagct gcctgtgcaa tacccagtgc ctgtgcagca
    28021 atgccaactc taccaccatc cagagtatgc atggcaattt taaaaccgtc gccttttttg
    28081 cccaaaaggt tttcttcagg gatttcacag ttttcaaaaa ctaattcagt tgttgatgat
    28141 gcacatatcc ctaatttatc ttcggttttg ccaactgaaa atcctggagt tcctttttcc
    28201 acaataaaca tactgaggcc cttatggcca acgcccttct cagtaactgc catgacaacg
    28261 gcaacacttg caacaccacc gttggtaata aaattttttg taccgttgag tacccacttg
    28321 ccattcttaa attctgcagt ggttttggta cctgctgcat cagaaccagc attgggttcg
    28381 gtgatgccaa aacagccaat ccattcacct gagcatagct ttggcaggta tttctttttc
    28441 tgctgttcag tgccaaagta ataaataggt gacaggcaca atgagttatg ggcagataca
    28501 ataactcccg tggatgcaca cccacgcgaa atctcttcaa cggcaatggc ataggtaagg
    28561 taatccatac ccgatccacc gtattcttct gggtaaacaa cacccatcat ccccatttca
    28621 cctaattttt ttacatgttc ccatggaaat tcatgcgttc ggtcataatg ttcagctttt
    28681 ggtgcaagtt cgttttctgc aaacttcctg cacatatctt tgaccatctg ttgttcttca
    28741 gataaattaa aatccataag ccgatctcct tatactatct ctataaatat ttgctgtgta
    28801 ctatcgtttc atatatggat ttattttgtc aaggcaataa caattgacag gtaaaaagca
    28861 tggatttttt atgttgaaaa aaaggctggt acatactaca aatggttaca gtatatacgt
    28921 ggcgatagtt actgatgagg gatgaacagt atggttaaaa aaatattgat ggcaatagcc
    28981 tgtagcatgc ttatttcatg cggttatttc agggtgaccg atctttcagc aaacagggtt
    29041 tgtgcaattc cgtcaggagc ggcattaggt gcggtaagca ttaactataa tgagtcagga
    29101 cttcttgata tgagttttgg cgtgcttctg gcagatgata aagtttatgt taaggataat
    29161 atccagaaga aagtgcttgt ttttgacagg gatgggaaat gtttaatagc tattggcaat
    29221 gtcagtgcaa cagcagaagg aaaagacata aaaaaaacac aggttaaatt tgacatcata
    29281 ggcactatgg ttgccgattc agatggcaac ctgtatgtac aaaacaggct ggcctcagtt
    29341 cagcgaattg acgctgcagg gacgcagatt gatatgtcac cttcttttat acttgtcttt
    29401 gatgaagaag gtaaactgca atacaccata ggccagacgg gaatgccaac agttcctttt
    29461 tactacattg agcatttaga aatagacaaa aaaaacaggc tttttgttat tgcacgtacg
    29521 tatgatatat ggagcgtgta tcgctttgac aaacgccagc gggattatta tatgacattt
    29581 tccagaaatt actttgtaga tacagttgat gccacaacat catataccgg aaaaatagaa
    29641 aatatcagga tattacatag cggcgatagt ttcttgcttt ctgttgccta ttacaataac
    29701 acccggttta aataccgaaa gttatatcag tttgatatta aagaatcaaa acctgttgaa
    29761 ttgttaaaaa tttcagaacc aaagaatgaa ttattcacgg tagcacagaa taaatatatc
    29821 tatgtgtgga atgttgaaga aacaaacgaa gtaaagttta tgctgtataa ttttgacggt
    29881 acaataattg ggaatgtgcg gtgcaaattc ccagggcagg cattattttc ggatattgat
    29941 attaccagtg agggtaaatt atattcatac catgcctact caacagggtt agaagtatac
    30001 gaatggaagt gatgcatgaa ggtagttaca gcgcagcaaa tgcaggaaat tgacaggatt
    30061 accatacagc agtatggaat acccggtgaa gtgctgatgg cactggcagg taagtcagtt
    30121 gctgattatg tattgcatac tgttaaagcg cataatgttg cagttttctg cggggtaggc
    30181 aataatggtg gtgatggatt tgtcgcagcg tatctattgt cacagtattt acctgttgat
    30241 gtatttatcc tgggcaacac tgataagttt acaccatcat caaggatata ctatgatgta
    30301 tgccagcata ccagtgtgcg ttgttgtaca atatcccctg aaacactggc aacaataaat
    30361 ttgaatagct atgattgtat tattgatgca ctgaccggca caggattttc cggcaagcca
    30421 aaaggtttgt tagagcaggt cattaatgtc attaatcaaa gcggggaaaa ggttattgct
    30481 atagatatgc caagcgggct tcctgcagat ggcgctgttg aatttgaacc cattgtacag
    30541 gcacacacca ccataaccat gggactcccc aaaattaatg cagtcaccta tcctggcaag
    30601 tactatgcag gcaatgtcat cgttgctgat ataggatttc cttattcact gtgtacatca
    30661 ggggatattt gtcggcaact tattgatgaa aagtttttta ccggtaacta tcgccccaat
    30721 atacattatg atgcccataa aggtgcaaat ggccatcttt tgtgcattgg tggatttgat
    30781 ggtatggagg gtgcaattct tttatgtgca aaggcagctt ttgcaacagg catagggttg
    30841 gtcacgatag taacaacaca ccaggcacgt gcaattattg ctggtaaaat tcctgaagcc
    30901 atcacgcgcg cattgaacgc tacagccagt gaacccttta caaaagatat gtacggtact
    30961 attgagttta ccaatgtata taaagatatg ctatcacagc ttgaaagcat aatacagctt
    31021 gcacgcccca ctgcgtgtat cattggtcct ggcctgggga gaagtgtgag ttcagcagca
    31081 actattgcag cgtgcttgaa aattgcgcac acaatgcagt tgcccgtact tattgatggt
    31141 gatgggctgt attgtctggc gcagcttcct gaagcaaagc ttcctctaca ttgtgttatt
    31201 acgccacatt ttaaagaagc agaacgctta actggcatgg cagtcagtga cataaagaaa
    31261 aaccgcttac gttcagcaga agacatggca caaaagcttc aatgtgtgac agtgctgaaa
    31321 ggccctgcat ccatagtaac cagtggtacc accacgtata ttaacacaac cggtaatcca
    31381 gcgcttgcta caggtggctc gggtgacgtg ctttctggta ttattggtgc attgctttct
    31441 aaaggatatg atccgttgat tgctgcagca ctgggtgttt atattcatgg caaagctgct
    31501 gatatatact gtcagggaag tggtagcagc atgatgcagg caggtgatat tgtacggttt
    31561 gtacgcaacg ctttgaattg ctaattacta catgatactc acatcactta tatccagttg
    31621 agtttattgt ataatattgt ctatgctcac aaaccgtgcc tttttacttt tttctttctt
    31681 ttcaaaatct tctataattt ctttatcctt gatgtcttca taaagctccc ataatgattt
    31741 tttaataagt gatgatgtat cacaattata atgcttttgt aacagtttaa gtattttttc
    31801 ttcctttgca ttaaatctca ctgatgttac tgccatgtat cgtatacctc ctcgcgtttt
    31861 ccaatgtaaa ttacataaaa atcgttattt tccatataaa aaatagctct atatttacct
    31921 ttacgtaatc tataataatc tcgtttgtaa ttccctttta gtttttttat gtcaaataaa
    31981 ctaaagtctt ttgttaaatc aagcgctttg aatgcattgt gaaacagctg tattgttctt
    32041 aaaggtattt cacctttctg aatagctttc tgtactgatt tctcatataa tatcatgtaa
    32101 acaatgtatt acatcagggg tgcattgtca ataaaaaata gtattaccgt gcactcatga
    32161 caaaaacgtc ttctaatgtt ggaagtatgg gttcaatggt aacatcatga atgttgtgtg
    32221 attgtagtaa tttttttatg atagtaaaat gtttatattg tttttcaagc acaacatgaa
    32281 ggtcagttcc aaaaagagat acttcaataa caaagttttc tttactcagc acattgtaag
    32341 ctttcaggta actatcggtt acaattttta caatagtgta gggtagtttt tcttttaagc
    32401 cctgtggtgt atcaagggca ataattttgc ctgcaataat aagcgcaatg cggttacagt
    32461 gttcggcttc atccatgtaa tgggttgtta caaaaacagt tttaccttcc tttgccagtg
    32521 tataaatttc atcccagaag attcgtcgca tcaaaggatc aacgcctgag gttggttcat
    32581 ctaaaaaaag tatctgagga tcatgaatca gtgccgagcc caatgcaagg cgctgcttaa
    32641 tgccaagcgg caggtttttc accaggctgt gcctgtagtc cttgatattt accagttcat
    32701 aaataaagtt catgcgttgt tgtaaaaggt ttgcggaaag cccatatacc gtaccataaa
    32761 attccagatt ttcttcaatg gtaagatcct catacagaga aaatttttgt gacatatagc
    32821 caattatttt ttttatgtcc cactgctgat gaacaatatc aagccctccc acgcttcctg
    32881 taccttcatc aggctgcaat aatccacaga gcatttttat agtggtgctt ttaccagcac
    32941 cattggggcc aagcaaccca aaaatttcac cttgagctac agaaaagctt actctgtcaa
    33001 ctgcggtaaa atcgccaaag cgcttggtga tgttttcaac tatgactgtt tccatagtta
    33061 gcctgtcctt tttccaaact gccagacgct tatcctgagt attgttattc ccataattaa
    33121 aagtattagc atatgttgcc acaggcatgt aattccagtg ccttttaaaa atacactgcg
    33181 aacaatctca acaaaatagc gcatggggtt tatatatgtt ataagctgaa aaattaccgg
    33241 catggagtag ataggaaata taacacctga aaacaatatt gcaggtacaa aaaacataaa
    33301 tgatgacagc atggcttgct gctgcgttga tgacacggtt gatatgaaaa gtccaatagc
    33361 taatgtggaa ataatgtaaa ttgcaccaca cagcaaaaga aagacaaagc tacccctgaa
    33421 aggaatgcca aaccagaaaa tggcaatagc ggttatgaca ataatgtcaa caaatgcaat
    33481 gatggcaaag ggtattaatt tgccaatgat gtattcattg ggcttcagtg gcgatacttt
    33541 aatctgttcc atggtgccag cttcccgttc tttaacaatg gacattgccg tcaacatgat
    33601 ggtaataagg gagataagca tcgcaatcac tccaggaaga taaaaattga tgctttttaa
    33661 atcctgatta aaccagtacc gtggaataag ttcaatggta cctgatgaaa tgccagattg
    33721 taaccgttgc atcactttca ttttaatggt gctgatgaag cgttcctgca catatgaaag
    33781 tgtcagcgca ttgatgtatg actggataac agaagccctg cttgaatcag tgccatctaa
    33841 aattacctgc aatgcaacag gcttgccaat ctttacattc ttgccaaaat tattggggat
    33901 ctctaaataa aaatcaatat cacctttttc tatcaatcgc agtgactgtt ttggcgaatc
    33961 caggtatgca tacatttcaa agtaagggga tgcaataaac tgttgtagaa attctctact
    34021 taatgaggta cggtccctgt caaaaacagc aaacgataca ttggtaacat cggtatttac
    34081 cgcataacca aacaccagtg acataaggat aggcggcact atcataattg cccgcagttt
    34141 agggtcgcgc atgacaaggg taaattcttt ttttatcatt atccttatat tgtgtaacga
    34201 tagtttcatg gttactatcc tgcataccgt acattaattt ttttaatgcc aatgataatc
    34261 ataatacatg caaacactgt cataaaaaca atctgagttg taagcagtga tgcagccaaa
    34321 cctttcaagg caataccttt tatgagtgca atcatgtagc gtgccggaat gatgtatgtt
    34381 atagcttgca gaatgattgg catattcttg atgggaaata taaaacctga taaaagaatt
    34441 gttggcaggt acgttgctat cattgctacc tgaattgaaa gtacctgtat gcgcgtagcc
    34501 gaagaaataa ccagcccaaa ggtgcaggtg ccaattaaaa acagtattga aacaagcaaa
    34561 agttcggcaa aacttcctct gacgggaata tcaaacacaa agtagccaac aaggtaggta
    34621 atgaccacat caaatatgcc tatgaaaata taaggaatga gtttgccagt aatcacttcc
    34681 cacgagcgca caggcgtagt cataaggctt tccatagtac cgcgttccca ttcacggctg
    34741 attgcaagtg atgtgatgag tgcgctgatt atggcaagca ctatgacaat gatacccggc
    34801 actacaaagt ttttactctg caattcttca ttataccaca ctctactttc aatatgtacc
    34861 ggtaactgta ttgagccaaa ccctgcacga ttcaggtgtt cctgcatcca ggcaatgttg
    34921 tattgtgata caatagcatt aaaatatgct gaagcgatag tggccgacat ggaatctgat
    34981 gcatctacta taagttgcag acgtactgat tgcccgcttt ttacgttttc agtaaaattg
    35041 tacgggataa ccagcgccgc aactgctttg cctctgtcaa ttgctgtatc aatatcatgg
    35101 tatcgggaga catatccttt gaacgacagg tattcgctgt gcataaaaat ttcggcaaat
    35161 tgccgtgatg aaggcgtatt gtcctgatcg tgaatgataa aggcaacgtt tttaacatcc
    35221 atattcagcg cataaccaaa tatgagcact aaaaacgcag gtgtaatgag tgcaagaata
    35281 aggctgcgga tatccctgta gatgtgaatc cattcttttt gagctatcgc taaaatgcgt
    35341 attagtgttt tcatattact gtgcctttgc tattgcttcc acaaatgtgt cctcaagtga
    35401 aggaataact tcgcgtatgg aaatcacccc aatatggtgc ctggtgcaca acatgctgat
    35461 ttcctttttt gccctatgaa cattgtccac aaacacacgt actgcattgc cactgcgcac
    35521 agcatgcgca tacgtaaggt tttgctttaa catcttttcg gtttcaacgg gacttttgct
    35581 ttgaatttca atcagtggtt tgccaatcat tgcctttacc ttagtgggtg tgtccacggt
    35641 aaaaaaagat ccattataca tgagtgcaac cctattacag cgttcagctt catccaggta
    35701 tgcggtactt accatgatgg taaccccttc aacacacaga tcatacaaaa tgcgccagaa
    35761 ttcacgacgc gataccgggt caacgccgtt agtaggttca tcaagtaaaa gcacaagagg
    35821 ccggtgaaca agacagcacg caagacctaa cttctgtttc atgccgccgg aaagtttacc
    35881 ggcaagtctg tccttaaacg gttctaagtt gctgaagcgc cacagtactg gtaatatttt
    35941 ttgaatttct ttttgacgca tgccaaagat tttgccaaaa aatatgatgt tttcaagtac
    36001 ggttaaatct tcgtacagtc caaagcgctg cggcatgtat ccaatgcgtt ctttaacata
    36061 gtagatgtcg tcatcatacg gcctgccgtc aaaatacagg ttgccactat ctggctgcaa
    36121 tattccggca gccatcctga gcaacgtgct tttgccggca ccatcaggtc caacaatgcc
    36181 aatgatttcg cctttgtgca gggtcaggct aacattgttt acagctttaa tgctgccaaa
    36241 tgattttgta actttactaa catctatcat tgttcacctg tctctattac agcatctgct
    36301 ggcattcctg gctttaattc ttttccggaa ttgtcaatgg caatctttac ggcaaagact
    36361 aactttacac gctcctcttt tgtttgaata gtttttgggg taaattcagc cttttgggct
    36421 attgcaacaa ccttgccggt aaaggtttta tccgggtaac tatcgacaaa cacaagcgcc
    36481 ttttgtccgt gtttaaccag cccaatatgc ttttcgttaa catagatttt aatccatgct
    36541 ttagtaaggt cagcaagcgt catgattgat gtaccgggga aggcaagctc acccacctcc
    36601 aggttacgtg aaagcaccac accgcttacc ggcgcataca gtactgcctg ctcaatggtt
    36661 gccatgacat aatcgtaatt accttttgca acgttgtatg cggtaacagc gttgtcaaac
    36721 tcacgctgcg atatagaacc ggtagtaaaa agttcacgtg ctcgtttcag gttcttttca
    36781 gcattgttaa ggtttgccag cgcggacagt ttctgtgccg atagctcatc atatgctaac
    36841 tttgctatta aatcaccctg tttaacttct tcaccttcgt caaaggggat ggaaacaatt
    36901 ctgccggcaa ttttagatgc aacctcaatc tcggtgactt caattacacc ggagccttca
    36961 atacgcttac cgtttgtgcc tatataacgc acaaactcaa aatacagcgt tacgccaact
    37021 acaataacaa gtacaataat aacaggtatt atacgtttat ttttcattgt gcacctccgc
    37081 aaatataaga tcagtacaat caaaaccggt gtcctttttt agctgagcaa ccgcatacca
    37141 gaaatcattt attgccttaa tgtaacccat gcgcgctgat gtaagctgca gttctgcatt
    37201 gagtagatca gcgtttttta taacaccagc cttatagctt tcttttgcaa cacgtaatcc
    37261 ttctgtagca gtttcaatat ttttacgctg ggataccatg ctctgatagg cagtcatgag
    37321 cctgccgtaa tcagcacgaa cagcaatagc aattgcctgt ttaagctgca atatctgctg
    37381 gttaatttct tttagctttt ctttttgttc acgctccaca ccctgcacgg tatcaacggg
    37441 agccagtgca ccccatctat aggttgcagc aacgcgcacc tgccaggtag tctgccactg
    37501 cctggtcccc gttattgcag ataagtctat gctttgcccg ggaaaaggta gctgaatctg
    37561 tggagcatct ggcataaggt aacttttgcc ataatatcct gctacactga atgttggcca
    37621 caggtacacc gattgtgcag catcaatact atcaccaaca atatctctgg ttaaagttag
    37681 ctgaattact tctggcctgt gcataagagc cttttcaacc agcgtatcaa caatgtcata
    37741 tccatgtggc tcttttatag aactatcaaa cccttcaaaa ataacggtat aagtgccgtt
    37801 tttagcaccc aggtaatagt taaaaaggtc aagtgcagtt gaaaagttat tacgtgcctc
    37861 aatgagtaaa ggttcctgac tttgcaactg cacctgagcc tggaggagtt caaattttgg
    37921 gattgtgcca gtattaaaca tctggttgac gtcagctaca ttagcctgta atacagcaat
    37981 tgagttttct ctgagtctta taatttcgtt agcagtgatg acgtcaaaat agcttttgat
    38041 gacattgtat tcaatgctgg attttacttt tttaacttct tcttttgcaa gtttgtaatt
    38101 gttgtagctt tgcgatagtg aattataaaa ggtagcggga ttgataccaa agcgcacctg
    38161 cacaaatttt aaatcatact gaccgtcaga aaggctcata aatccatttt cagcatactg
    38221 tcgcatggcg cttgcttcag attctaatgc cggatacagc attccccatg cctgggctat
    38281 tttttcgtca gcctgctgta acctgtgttg tgccatgata tattccgggc tgtttttaag
    38341 tgctatatca attgcgtcaa tcagtgaaag tggctgctct gcatgtgcgt atgaaataaa
    38401 aagcaaaaaa attatgggcg atagtacctg tataacaaaa ccttttgcct tcatatatcc
    38461 tccttgataa ttccatgtga catcaattgt aagagattgg caatatattc atgtacatga
    38521 ttgtcactaa aatagagcgc atgaagagag gtgccctgta aaatctgtcg catgagatta
    38581 taaaagataa cagcaccagc gaccgaaatc atggtatatt gtggatgcag ttttggatta
    38641 atacgtttgc ttttcacagc ctcaaaaaga ttgccaatac gctcagtaac aggctcaata
    38701 atagttggag caatgatatt ttttataaat ttcccgccac ttgcaagctc acgcatgata
    38761 atcataaaat agcgcctgtc taaattgcta atatggctgg catatgcttc aataatgata
    38821 agtggatttc cggtttcctg cacctttgca atactggtag atattgtatc aacaatagtc
    38881 tgtgcaagtt cctgtaaaat tgaagcatac agtgcttctt taccggggaa atgataataa
    38941 atcattgcct tgtttaccct ggctctttta gcaatgcggt caatgcgagc accatccagc
    39001 ccataccggg aaaattcatc aattgctgca ttcagtattt tgtcttttga agatgccatg
    39061 actatcttat gttaactaac tgtttagtta gcatattggc atctgggtta aaaaaatcaa
    39121 gattttttta tttattcact taaatatatt ttttaataga atgattacaa aagtttatga
    39181 taagtttctg atatatgggg tgcatcaaat agcaataagc aaattttaaa tgcagattca
    39241 agaataaagt gcaccacaca atagaaaaca tgtagcatca tacacagcac atcctttaaa
    39301 gtgggtgttc caacaacaaa atctaaaaag caggtatgta tgaggcatta ctcatataca
    39361 atcaggagaa agaattgtca ttctaaagat tcacaggaaa aagaatgtgc catcagcata
    39421 aaaagtataa ctatataaag aaataagtga aaagacaaac caagatgttt tttaaaaaca
    39481 gcaaaggtgg ataaaagcaa aagtaggggg caatagtata acaataatga taaatacacg
    39541 tccaggtaaa tctaaactat acaaatcttt gtatgagttt tacagagggg actacatggt
    39601 gcactgtaaa tgtgaatttg caatgaaaaa ttatagtgaa aaaaattttt atttgatttt
    39661 ataaatggta gtattgtacg ataaacaaaa atataaaata tataaattgt ttaaaattat
    39721 atgatacaaa atatatgagg tatacaatga aattaaggac tttattaatt ttatgtgtat
    39781 attttatatc tttttttgaa tgtattgtat ttggtaatct tttagctata aatattacag
    39841 aaatacatgg atggaagtca cttgaatcag atattgattt cctgtgggaa aatcaacata
    39901 tgtatcgata ctttcagcca aattggaact atccaattgc taaggaacag gttgttgaac
    39961 ggttagaaac cctattaaag aaattagata aactagaagc aaaaaatagt agaaacatag
    40021 aatactacct tttaaagggt gatattgctc attatttata taaccttgat aaaacacagt
    40081 attataaaaa agctcttatc atgtacgaaa aagctaaaac aattgagccg cgcgattata
    40141 gaccatattg gtttattggg cagcatgaat ttttatcggg acataacgtc aaaggatatg
    40201 aaagctttaa gacagttatt gacgttattc caccggataa atttcattgg gcattttggg
    40261 gcgattattc caattgtgct tccattggca tgatgccatt taatttttta atggcatttg
    40321 aatttatgca taagaataag gccgaatttg aaaatatcga tcaagaacct ttttttaata
    40381 catataacga tgtaaagaaa agagtaacca tattagatcc tgatggtgag tatgaagtac
    40441 agaaaatatg gacaaaggtt ccatcggaag agatatacat ctcaacacct ctgggaatga
    40501 gaattcatgc aaaaaaaagc tggcaaataa acccaaaagg tcttcaaaaa aggtactctg
    40561 tgatgatgat gttctttgat gccgttaaag gaaataataa tgaagatata ctggctggtt
    40621 taagcatctt tacctactta cctaaaaatg gagaaacgct ggaaacacat atgaagaaat
    40681 atttagatgg gtttaaaaat ataagaaagg ttacactgaa caataaattt aacacaacac
    40741 atacttatga atcatctatg tctgaaatag ccagagaaca ggatggcatg agagtgtttt
    40801 ttatttttct ggaacgggat gagcctgcat atccctgtgt aaagattgaa tatccctata
    40861 caccacgcga taccggattt ttttatttaa aagaagatgc aacatttgcc agtagattta
    40921 aaggtaaaat tttttacatc atagtctttg aaaccagggg tagtgtttat agcaaaacat
    40981 ataaaatgtt taatgatttt ataaatacaa gtctttattt tgatgaataa caaaagtgtc
    41041 tatatatgat ctgaccccct ttttttaaca ggatatacag gatgtacagg atatttaagg
    41101 atttatcttt attatttcta ttccttatta tccgatgtat cctgttagag ttataaggct
    41161 ctgaccccat ttttttactt ttttttataa aaaataaaaa ttttttccac aatcccgtta
    41221 atttttttca ccacctgcgt tatatagagt gagagaaaca cacgtctctc atagtacatc
    41281 agtgtatgga ggaatactta tggcacggaa attgcgtgta caaatgcccg gtcttaccta
    41341 tcacatcaca agccgctgca ttgagtggcg aaagatgttg gaagaagagt atttcaaagc
    41401 ctgtcttgtg gaaattttac gcaaggcaaa agaaaaatac cggtttaagc tgatagccta
    41461 ctgcattatg gacaatcaca tccatctggt cattc
//
LOCUS       DAORYL010000018        38731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_853_length_38731_cov_14.632046, whole genome shotgun sequence.
ACCESSION   DAORYL010000018 DAORYL010000000
VERSION     DAORYL010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 38731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38731
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_853_length_38731_cov_14.632046"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PLV81_05315"
     CDS             <1..216
                     /locus_tag="PLV81_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09552.1"
                     /translation="WLSHGKRRSLQHHHGNMQTPCKKVHWHTVSEQKKKRSKKKWKGL
                     IIGNYRPWVTEPFCIVNFIIINELKIR"
     gene            301..1461
                     /locus_tag="PLV81_05320"
     CDS             301..1461
                     /locus_tag="PLV81_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOM09553.1"
                     /translation="MGVRAIKKVTVIGSGAMGSGIAEVHIVNGFNTVMVDINNDLLQK
                     AKEKIKSNLSFLVNKQKMTQVAMDEAMARLATSTDITSSVKDADCIIEAVPELMDLKK
                     KIFKTVSDAARTDAIIASNTSSLSISELAETVSEPSRFAGFHFFNPVNRMRLVEVIYG
                     QKTSDEVIDTLMDLGEKLGKVAIKVLRDRPGFIVNRINAPLQTLWSAILDEGKISPAS
                     IDTVMKNHGAKMGPFELMDFVGLDVIYNVMQYYKTTLSPEWEPGKFITECVKKNELGM
                     KTGKGIYVWEGGKAIIDSSIVTDIIKPIDPLAVQLNEAVRVLKEKVAASAEDIDKGQE
                     AGMNQPGPFKTAMSIDNKLLAERLNWMSKTYNLTYLKPEPEILDGSFKSFMR"
     gene            1471..2244
                     /locus_tag="PLV81_05325"
     CDS             1471..2244
                     /locus_tag="PLV81_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOM09554.1"
                     /translation="MSKNECIITEVKDSVGLVYIDRTEVMNALNRTLIVELGNKIKDM
                     CADAGVRAIIISGKGDHFAAGADIGEMAQQNPHQALAFSFNDVYTMIEQLPVPTCAAI
                     KGYALGGGLELALACDFRICHTSAKLGLPEIKLGIIPGAGGTQRLPRLIGLSRAKEII
                     FSGEFITAEKAYQWGLCDRLTDNDPVLEAVSFLSTIIKRPSLALQAAKKSIMFGLDSS
                     LKEGLEYEALSFGILFSTHDQKEGMKAFIEKRKPNFKGC"
     gene            2255..3439
                     /locus_tag="PLV81_05330"
     CDS             2255..3439
                     /locus_tag="PLV81_05330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003387762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOM09555.1"
                     /translation="MDNKDVVIVSACRLAIGKFGGMYANTSALELTIPVMQALVQRSG
                     IDPELIDDCIWGCNYQRTNGENNLARVALVKAGLPITVPGITVHRNCTSSMSSVQLGY
                     YQIKAGEADCIIAGGTDSMSNAQYTIDKMRYGTRIGHTEIRDSMWDSLTNLGIGPAMG
                     ITAENLATKYNISRKEQDELALLSHKRAIAAIDKGYFKKEIVPVEVKGQKGTIIADTD
                     EHPRRDVTIEKLAALKPAFKDDGTVTAGNASGINDGAAGVILMSAKKAREVKAPIIGR
                     IISTATAGVDPDIMGIGPVPASRKALEKARLTLKDIQLVEINEAFAAQYLACEKELGL
                     NREITNVNGSGISLGHPVGATGVRLIVTLLHEMKRRNVQYGLATLCAGGGMGTAVVIE
                     HI"
     gene            3460..5301
                     /locus_tag="PLV81_05335"
     CDS             3460..5301
                     /locus_tag="PLV81_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOM09556.1"
                     /translation="MRPNLLVDSRDVRFVLFEMFDLVSLSEYEPYKEFDRQTYEAVLD
                     LAEQIAMEEVYPANTIADKQGVTFNPDTHTVTVPEVFKQAMKVFNEAGFTGLVQPISC
                     GGMGMPEMMYRSALEYFLAASISFTIYITLSNGATNLVRIFGNEEQKQLYLQNMVTGR
                     WGGTMCLTEPQAGSDVGAIKTKAYRQPDGTYKIKGQKIFISSGENDLYENIIHMVLAR
                     IDGDPEGTKGLSLFLVPKILVHSDGSLGNRNDVICTGVEHKMGIKASATCTLSFGDND
                     NCIGYILGKERDGIKNMFHMMNEARLDVGLEGLGVSSAAYMHAVQYAKSRIQGKTISK
                     NEPSIIGHPDVKRMLLWMKSHVEAMRMLVALIAYHIDISRVRNDEIGKKAQALVEFLI
                     PICKAGNTDLSWLITAEAVQVYGGYGYCGEYPVEQYARDSKILSIYEGTNGIQSIDLT
                     MRKILSDEKRERYNAYKDYASDVIKKARAIVPENIINQVTIAIAEMDSLIDVLAAKDT
                     AFALSVATMLQQAFRILTHGILHLNSMTIAIQKLQSLAGGYTYNQVPEGLEDNSEIAF
                     YYGKVLSAQFFLTVEFEKYSGIIQGIRNENGVVLRAGSSIFTGVLEA"
     gene            5240..6196
                     /locus_tag="PLV81_05340"
     CDS             5240..6196
                     /locus_tag="PLV81_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HOM09557.1"
                     /translation="MKTELYCGLVHQSLLVFLKRKTMQIHPSIYIHNADITPRGKKAL
                     IVEGGGMRGVFSAGVLFAMGLRGLTHFDLYIGVSAGACNLASFLGRQYERNFYIMENW
                     SASWRFINPFRIFWGNVLDLDWLWDITISNYRLNLHEIFKDSSREFLVVVTSMESGKA
                     LYIKPNKENLEFVLKASSALPVLYRNKLFIEGQRVADGGVGDSIPVKEAYKRGARDIV
                     VIRSQAKEYRKDKSESGTLLKLLFRSYPEFVKALVQRPVNYNKAIEFIENPPDDTRIT
                     EICPPAWFKTKRTTTSKQQLLKDYYSGIQTGFLLCNYYKSLQ"
     gene            6212..7870
                     /locus_tag="PLV81_05345"
     CDS             6212..7870
                     /locus_tag="PLV81_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOM09558.1"
                     /translation="MERLWEKNYDFWYHSVNFQRMTVQDMLHCSAAVAPDKVATDFFG
                     TQITYYQLRLLSIKFAQALVKIGVKKGGRVCLQLPNSPQFAIAYFGTLMAGGIATLMN
                     PLYTADEVNQLISITQPNAFITFDMILPNQRDVLKQSGVTVIVTKITDFIDGFPKGSA
                     QELQLEKNWHYFSDLLDSIDEASVPKIDISPDDPAIIVFTGGTTGAPKGAVHSHYNVV
                     GATISTMEVGKYFIDLMPAETRCNIGVMPFFHIAGHIQCFTWGIFRQTTMYLVPRFDI
                     NEFINLLARIPHKITYFFAVPTLLTALVNHPKAKELALDKKFGLILSGGASCPVPLIN
                     KMKDMGLFYIEAWGMTETCGIGISTPIFGIKKPGSIGIPMQESMIKIVDLEDGKTEVP
                     MGKPGELIFKSPFIMQEYWNNKEETAHVLKDGWLYTGDVVTMDEDGFIYIVDRKKDMI
                     IAGGYNVYPSEIDDVLLKHPKVDKAVTVGVPDTYRGETVKAFIVLKEGEVATEGEIIE
                     YCKQHLAAYKVPKIIEFRKELPMSSVGKILRKVLREEEIKKVTK"
     gene            complement(8041..8649)
                     /locus_tag="PLV81_05350"
     CDS             complement(8041..8649)
                     /locus_tag="PLV81_05350"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOM09559.1"
                     /translation="MKTKRIQYTMFNTPIVKHILRALAFMLRTLLGWKKVGRLPDFPK
                     YLAILAFHTSNWDVFYGILIAFGFNVDIYFMAKNQLFRFPFAPLVRFLGGIPIDRSVS
                     SNTVQATIDMYNKHEKFAIALAPEGTRKKVEKWKTGFYHIATGAHIPIILAFLDYKTK
                     SGGIGAILHPTGDIKTDFKTIRDFYTQVHARYPELASLPQEE"
     gene            8823..9506
                     /gene="nfi"
                     /locus_tag="PLV81_05355"
     CDS             8823..9506
                     /gene="nfi"
                     /locus_tag="PLV81_05355"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259813.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HOM09560.1"
                     /translation="MNMHWNCSVEEAIAIQKELAGNVIIAGKPEKIDHIAGIDIALTP
                     DNKGYCIIAIFSFPDMELCEEVFSSDTINFPYVPGLLSFREGPLVLKAYEKLSIKPDV
                     MVFDGQGIAHPRRCGIASHIGVLLDIPSIGCAKSRLCGTYIEPGQDRGARAYLYDKEE
                     IIGVVLRTRAHVKPVFISPGHKIGIDEAADIMMQCTGNYRIPVPTRYAHCRVTEYKRS
                     GVDTGLKSY"
     gene            9561..10169
                     /locus_tag="PLV81_05360"
     CDS             9561..10169
                     /locus_tag="PLV81_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09561.1"
                     /translation="MHPISGTNFTVYIHDLIHSWLKTLILLGSILVPGFLILDYVITP
                     HDLFPRFVVYRCVSTALLIIQYVILRISKPGRFSFIHGYLAALNTGFVIALMTVDLGG
                     FSSGYYAGLNLVIIGVNLLTPWPFIHSLINGLAVVCMYVGLNVVSSQSTDYIYMINNL
                     FFMVSTVVITASFSFLRFKQLKSEFDLTTIILLTNRSVQFTL"
     gene            10287..11612
                     /locus_tag="PLV81_05365"
     CDS             10287..11612
                     /locus_tag="PLV81_05365"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOM09562.1"
                     /translation="MEQKKIIIAGLGSAGFAALVTIKRINPKAHVVVIDPKDKDIVHP
                     CGLPYALEGKVNEQDLQQDIFLSRMNVEKVKGIVHSVDAEKKEIAIQYDDKTITLQFD
                     TIILCTGYKPQLPPIAGIEHYIHKGVYTLSSIDDLKRIKNDIQGKGRAIVIGAGAIGL
                     EVACGLKAHGLTVTVIEAKEVLPQAVDSDMAQLVALYCANQNIEIITHSPVTAIEKRD
                     SEFSIRTPEKEYTADIVIVATGFAANCEWAVNSSFETGKWGVVVNEHLEIKPSIYAAG
                     DCIQNWSVIDNKPFAVKLATSAYKQGIVAAEHAMGLNSAYKGTAGTFVTKVGELEIAA
                     TGFSTQVAQERGFSVIAGKISATVKPEYFPENERLTFKVLCDTKGKLLGAQAIGNGAA
                     ARINIISLAIEHGLTIDDILPFEMAYCPAVSEVNDILFKACEFAKRRIK"
     gene            11609..12703
                     /gene="gcvT"
                     /locus_tag="PLV81_05370"
     CDS             11609..12703
                     /gene="gcvT"
                     /locus_tag="PLV81_05370"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOM09563.1"
                     /translation="MTAQRTFLYDDHIKLGGKMVEFAGWELPVMYSSIIDEHMAVRQA
                     AGLFDVSHMGEIVVKGRGARAALQKLIPTDMGRLEKGKSMYTCFLNDKGGIIDDLFIF
                     MLSDNEYYLVVNAATTQKDVEWLTQHCKTNVSIQDVSAATAKIDLQGPQSYNILKKII
                     RDDRLENLQRFHFFTGAYKGEPVLISQTGYTGEFGYELFCINNVASRLWNDVLEAGKE
                     YGIKPAGLGARDTLRLEACYSLYGHELSDTITPVEGGIGFVVSAQSEYIARDIVEYQK
                     VHGAPRQIICLEMLDKAIPRNEYRVLYNNQDIGYITSGGFSPVFKKGVAMALVTSGVV
                     SVGDEVAIVIRDKTSPAKVAQRPFYKYSGK"
     assembly_gap    12917..13016
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13240..13632
                     /gene="gcvH"
                     /locus_tag="PLV81_05375"
     CDS             13240..13632
                     /gene="gcvH"
                     /locus_tag="PLV81_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOM09564.1"
                     /translation="MSNIPENLKYTKTHEWIDVQDDYTGICGITDHAQEMLTDIVFVE
                     LPEVGIEVKSGEQVAVVESVKAVSDVYAPVSGRIVEVNKTLEDSPDLLNSDPYGKGWI
                     FKIDIKDKHELDELMDAAQYAEHVASGE"
     gene            13635..14954
                     /gene="gcvPA"
                     /locus_tag="PLV81_05380"
     CDS             13635..14954
                     /gene="gcvPA"
                     /locus_tag="PLV81_05380"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOM09565.1"
                     /translation="MSFLPNSDDDIRSMCNEIGVNSVEDLFVDIPSTVKNPDIQLPSP
                     LSEQEAMHSLLRIANTNKRVCSLVGGGIYHHYVPAVVDHLSSRSEFYTAYTPYQPEVS
                     QGTLAAIFEFQTYMCRLTGMDIANASMYDGATALAEAVLMAVRTTRKNNVLCSAAVHP
                     HYRQVLATYAWASDISLQIVPYKNGATDYNELSHIINDTVACVVVQSPNFFGVIEDIE
                     KVAHIAHEHKALCIATFTEAMSLGLLKSPGECGADIACGEGQSFGNYPANGGPLLGIL
                     ACKKEFMRTIPGRLIGKTVDEDGRECYVMTLQTREQHIRRERATSNICTNEGLCALRA
                     TIYLSLVGNRLVELARTNHAAASYCLEQLVNLGYERVFDAPFFNEFVIKINNAKNVQN
                     KLVDNGFALGIPLETYYPELSDCLLITATECTTKDDIDTLVKLLQTI"
     gene            14966..16390
                     /gene="gcvPB"
                     /locus_tag="PLV81_05385"
     CDS             14966..16390
                     /gene="gcvPB"
                     /locus_tag="PLV81_05385"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOM09566.1"
                     /translation="MQTIFSLHKQGRNKYTVPQFGAESYDPIPQHLCRGELNLADVSE
                     VDVVRHYRRLSSLNFGVDNGMYPLGSCTMKYNPKINDAVASLHQFTQQHPATIEELSQ
                     GSLHIMYSLQEMLCAITGMKQFTLMPAAGAHGELTSVMIIKKYFETKGEKRNIILIPD
                     SAHGTNPASVAMCGFTVKEIPSNSDGDVDIDALRAAMDNNVAAMMLTCPNTLGLFDRN
                     IMTIADILHSNGALFYCDGANLNAIVGKVRPADLGFDIMHVNLHKTFSTPHGGGGPGS
                     GPIGVVEKLTPFMPVPVIAKKDNQFYLDYERPQSIGRVHSYYGNFLVMLRAYTYIVML
                     GASGLKQVAEHAVVNANYLRVKLQKHFNLPYTRICMHEFVINDEGMPNGITTNEIAKR
                     LLDYGFHAPTVYFPLIVHGAMMIEPTETEHRDTLDAFVDTMIAIKNEANTNPEVLKAA
                     PHTTPVRKVDAVLAARKPVLKWEE"
     gene            16378..17229
                     /gene="lipA"
                     /locus_tag="PLV81_05390"
     CDS             16378..17229
                     /gene="lipA"
                     /locus_tag="PLV81_05390"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOM09567.1"
                     /translation="MGRVRTIPNWIKFSIPSGEQYKQVQKHVAGCGLHTVCLEARCPN
                     IGECFSKGHATFLIMGNICTRNCKYCAVTKGQPQPLDIHEPQNIARAVANLNLEYVVI
                     TSVTRDDLEDGGAFHFVKTIEAIRTAIPDTKIEVLIPDFKFSQQRSLTMIMDAHPDVI
                     NHNIEVVKSYYHTLRPKGNYQASLTVLAIVTEHGFIAKSGLMIGFGETMDDIINTLED
                     VYSAGCRVLTVGQYCASHRGAYPVHKYYTPEEFKEIETIAYQIGFMKVLAAPLVRSSY
                     RAGELFS"
     gene            17226..17510
                     /locus_tag="PLV81_05395"
     CDS             17226..17510
                     /locus_tag="PLV81_05395"
                     /inference="COORDINATES: protein motif:HMM:NF021921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate protein ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOM09568.1"
                     /translation="MSIHVVLKNPGEKLVHIDIDFEKSTISCIHISGDFFIYPEEAIV
                     DIEQALLGLPVDPQLIEQTIFTLIKNNDIEMVGISVSTIVQGIQEAFAQS"
     gene            17507..18259
                     /locus_tag="PLV81_05400"
     CDS             17507..18259
                     /locus_tag="PLV81_05400"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HOM09569.1"
                     /translation="MTTWRLIIHNTCAPSWNMAVDSAILRLTKIPTLRLYRWSKPAIT
                     IGYFQDIEKEVHIELCKKDDIPVIRRLTGGGAVFHHEELTYSMVHPLQGPFGKSSITH
                     SYALIAQPLIHALRSMGVDAQYRPINDIIVGNRKISGNAQTRKEGKLLQHGTILVATD
                     TEKMLQYLTIDPKKIKQEGNPVITLSEIIKGLSKESMFEQLIKAVISSFKELFAVDFE
                     EADITLQENDMAIELERSYFSNEQWNCRRLSP"
     gene            complement(18262..19137)
                     /locus_tag="PLV81_05405"
     CDS             complement(18262..19137)
                     /locus_tag="PLV81_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOM09570.1"
                     /translation="MQKHLTPETILMIIKFQKSELTEHHIYNLLASHIKNHHNARILK
                     KVAEDEKKHYEFWKSYTHSDVKPDRWKIIKYYIISRLFGLTFGIKLMERGEEQAQASY
                     KSLEPIIPEVKKIIKDEHAHEKELYILLEEEHLQYVGSVVLGLNDALVELTGTLAGLS
                     FALANTHLIALAGLITGIAAALSMAASEYLSSKAEDAKDALKSAIYTGIAYIITVVLL
                     IIPYFVAGHYLVALGITLVIALLIIFFFNYYIAIAKDLNFKKRFSEMAIISLGVACIS
                     FAIGFFIKKYMGIDV"
     gene            complement(19147..19350)
                     /locus_tag="PLV81_05410"
     CDS             complement(19147..19350)
                     /locus_tag="PLV81_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09571.1"
                     /translation="MSMNIKEVILYDADSLEYSGKILVEGTSWEFCDVSNDFLVKFTK
                     GMPLKAVLQCLISFNIVYDIIEM"
     gene            complement(19604..21061)
                     /locus_tag="PLV81_05415"
     CDS             complement(19604..21061)
                     /locus_tag="PLV81_05415"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOM09572.1"
                     /translation="MTKAKTPHTIEQIMENIQTGDDSIRNKAFEEIIEYGKDAIVPLI
                     TLLAHPDEQIRDSVIWALCELKEHAGQALIQELSNKNPVIRESAAFCLGICKNPHATK
                     ALIPLLKDENEKVRETAAWSLVKIGYDDPQTVQEIVNCLYDDSILVKDTARWILEHIG
                     EPAFEALLQQLLQSSRNIDDIIYILEKIASPQLDRLINLLTTTRDIQQLSYIIQLLGK
                     LREPDAIQYLIPFVNHSNRQIIIITIDALINIGELTIEPLFEKINDETIFPINEMMPL
                     FIRYKKLSVPKLIEMMQSHSLSLRIFAARSLGVIEDKRAIEHLIQGLSDPNPQFVEAC
                     ARALGAFSNRLIIKPLTDLILNENYPIIKSSAIYALSKSIDNNTAHSLLTSFHDAPLI
                     VKKRLIQLASYFPSEESETLLQQALTIDDEDCKMLAIQGLKHIGTEKSIDCVRSAIIN
                     ENPFISDEARTALKEIIERKQRGNIKTLDNYDDFI"
     gene            21343..21519
                     /locus_tag="PLV81_05420"
     CDS             21343..21519
                     /locus_tag="PLV81_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010632137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOM09573.1"
                     /translation="MDNTQKVIDAFKKSNEPLNASTIAKMTGLDKKEVDKIMEKLKKD
                     SVIESPKRCYWTLK"
     gene            21577..22332
                     /locus_tag="PLV81_05425"
     CDS             21577..22332
                     /locus_tag="PLV81_05425"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOM09574.1"
                     /translation="MATTKKRNSAKTKKSAAKKNISSKKRSSTSSHAPYVLVIMALAA
                     GLIFVLSLKTDTIKQIIPKTNTVVDANKHNSSTNDVSKNSKKDDNHKDFDSKEKLKQE
                     LSHKEKKKEDEVATVQAKVYFLFYNEKADKIELRPVVREVNARVPVKAVLEELIKGPT
                     KVEEKKGYVTAIPPTLKVIDVSIVNTIAFINFNGALEEGAAGNIMLNRLDQIVYTATQ
                     FDNVDGIHILINGQRKRFLGPDGISIAGPLKRH"
     gene            22348..22863
                     /gene="cyaB"
                     /locus_tag="PLV81_05430"
     CDS             22348..22863
                     /gene="cyaB"
                     /locus_tag="PLV81_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013036645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HOM09575.1"
                     /translation="MLEVEIKSRCSDLDGVKKTIVESGGTFISTEVEEDWYFNHPGRD
                     FGKTDEALRIRKVNDMVFCTYKGPKLGGKTKTRFEKEVQIHDAVAMQEILQRLGFILY
                     GTVQKKRDVYLLNEVTICCDDVKGLGTFVELEMISNNKEEAEKRLFALAEKLGLSDFI
                     TTSYLEMVKGE"
     gene            complement(22870..23277)
                     /locus_tag="PLV81_05435"
     CDS             complement(22870..23277)
                     /locus_tag="PLV81_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOM09576.1"
                     /translation="MPKKVFIDTNILIYAFSETEPLKRDTVISLLEFNEIILSTQVIN
                     EFIWVLNKKFKIHFTDIKKLITSLVKNFSIATIDMADIVYAIDIAMKYKFSYWDSLII
                     SSAIKNNCIILYTEDLQNNQVIKNKIKIINPFI"
     gene            complement(23270..23461)
                     /locus_tag="PLV81_05440"
     CDS             complement(23270..23461)
                     /locus_tag="PLV81_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09577.1"
                     /translation="MIAIKKDYHVKDKYLTIEIPDEFKEQEIEVIIRLKHPVKKEIFL
                     NKIKIDTKKWKFNREEIYA"
     gene            23605..24279
                     /locus_tag="PLV81_05445"
     CDS             23605..24279
                     /locus_tag="PLV81_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09578.1"
                     /translation="MYKKMMIPCIAIIMLFTGYAIFANEMLKQKIIAFEQSIPWSDVY
                     DGFKTRQHSWHQDVMMAGSPQELGKLLVELQSFIIKEKLGSDWNNIKGAWIAKTGSAT
                     TYDDIAASLKQLQDYYLSSARQDGSDVKKEIITFEQYVPWEDVKDGFKTRQQGWHYDV
                     INAKSPQQAGLLLLEFHDWIYPDKFSDAWTVIKPAWVKKAKNAASWQDVKNLLKELQW
                     NYRKGK"
     gene            complement(24366..25778)
                     /gene="amt"
                     /locus_tag="PLV81_05450"
     CDS             complement(24366..25778)
                     /gene="amt"
                     /locus_tag="PLV81_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOM09579.1"
                     /translation="MRLLKISGIIGVVLLCAGLLLAQETNTIVTQVQADSVKMAVVAD
                     TLWVLFAAFLVFFMNLGFAMVEAGLQQAKNAVNISAKNFVVFAISSIAFWFIGWGLMF
                     GDGNGFMGWKGMFMLNGADNSPVTGEMYKGVYSSLNWTGVPLYAKFFFELVFCATAAT
                     IVSGAVGGRIKFIAFIVFSFILTSIIYPIAGHWIWGGGWLAKRGFWDFAGSTVVHSIG
                     GWAALTGALVLGPRIGKYNPDGSVNSIPGHSLPLATLGVFVLWFGWFGFNPGSTMAAN
                     AADIGRIAVTTNLAAAAGTLTATITAWLALEKPDLSMILNGALAGLVAITAPCAWVTP
                     GASIVIGGIAGILVVVFVLAFDRWGIDDPVGALSVHLVNGIWGTLAVGLFAAAPYAGG
                     SGQPPLGLFYGGGIGSFANQLVGVVAVGSFVFVLSWVIWIIINATMGIRVTREEELIG
                     LDISEHGIEGYPDFESYTLK"
     gene            complement(25788..27251)
                     /gene="glnA"
                     /locus_tag="PLV81_05455"
     CDS             complement(25788..27251)
                     /gene="glnA"
                     /locus_tag="PLV81_05455"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOM09580.1"
                     /translation="MEAIFDPIYLVNGHKEHTSISDILKIIKERNIEFLDLKFTDLFG
                     RLHHYTLTADRADEELFAVGTGFDGSSIRGFQSIHESDMLMRPDPNTAFIDPFFDHRT
                     ISFLCDIIDPVARRRYTKDSRYVGEKAIEYIRSTGIADMAYFGPEPEFFIFDKVQYEQ
                     TQNKAFYEVDSSEASWNSARGEQNLGYKIPYKQGYFPTSPHDQLHNLRSKMVEVLRSC
                     GIKVNLHHHEVATAGQTEIGLVVDELVRQADALVMYKYIIKNVAHRYNKTVTFMPKPI
                     FMDNGSGMHVHVSLWKENRNIFYDFGRYADLSDYARYFIGGIFAHIEAVLAFAAPTTN
                     SYRRLVPGYEAPINLVYSARNRSAAVRIPMYSTSEKAKRIEFRCPDPSANPYLAFAAI
                     IMAGMDGIENRIEPPDPIDEDIYELSPHEKRNIRNTPGTLKEVIDGLERDCSFLLKGE
                     VFTVDLIESYIAHKRLHEIDQIALRPHPWEYALYYEI"
     assembly_gap    27325..27424
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<27425..28320)
                     /locus_tag="PLV81_05460"
     CDS             complement(<27425..28320)
                     /locus_tag="PLV81_05460"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOM09581.1"
                     /translation="MEMYIANIDWALVISIIIIAIAAILIFIKVNLKICEPNEILIFS
                     GRKRKLHSGEVVGYRVIRGGYGLRVPLLERVSRLSLSIIPITLEIEGALSNGMIPLSI
                     VAIAHVKIASKEGGGLENAVERLLGKPSSEVETIAKNTIEGVLRGVIATFTPEEANYQ
                     RIELEKKVYDLAKDELMKLGFTLDSVKIEEIKDSQGYLDAVGRQRNAIVKRDARIKEA
                     EAEAEAKIVEYNARKKASDVEFQSRIAIEEYENNFRERKALLNESTFQKEVRAEYAKK
                     LAELKHLNELEELKKDVNAKKYT"
     gene            complement(28342..28683)
                     /locus_tag="PLV81_05465"
     CDS             complement(28342..28683)
                     /locus_tag="PLV81_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOM09582.1"
                     /translation="MKLIIAIIRPEKLADVKKELNEASINLMTVINSIGSGRQKGYPE
                     TYRGVKHEIRLLKKIELQIAVNDDYVEPTIKAITRGAKTGNIGDGKIFVIPIEECVRI
                     RTGERGSRAIG"
     gene            28999..29436
                     /gene="mraZ"
                     /locus_tag="PLV81_05470"
     CDS             28999..29436
                     /gene="mraZ"
                     /locus_tag="PLV81_05470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOM09583.1"
                     /translation="MFMGEYRPTLDEKCRVAIPIKLRKAFGEDNIIHKLIITHGFDKC
                     VMAFREVDWKEFVETKLVPLSQADPMNRMRIRFLLGGATECELDKQGRILIPDYLLEY
                     AQISKDVVMLGLYNRIEIWSAEIYQQYKPDGEALNQFAHDLGF"
     gene            29466..30416
                     /gene="rsmH"
                     /locus_tag="PLV81_05475"
     CDS             29466..30416
                     /gene="rsmH"
                     /locus_tag="PLV81_05475"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOM09584.1"
                     /translation="MEESTQYYHTPVMPQEVLAFLSGIEGIESSIVVDCTVGEGGHSE
                     LILKTWDGLKLYGFERDREILAIAHKRLSPYGNRVVLINDNFKNLWLHCKGMENRVAA
                     FLYDFGISSFHFDGSSRGFAFKTQEKLDMRLDDSVTLTAYDVVNNFSENELTQIFSTY
                     GQERWSRRIARKIVQKRSEEAIETTEQLANIVLQAIPKQYRVKHIHPATRVFQALRIF
                     INDELSAINESLKHALGYVKPGGRIIAISFHSLEDRIVKERFRRWAKGCCCDNDGLLC
                     QCTANPLVTVLTKKPLQPKESETRQNPRSRSAKLRVCQKV"
     gene            30422..30715
                     /locus_tag="PLV81_05480"
     CDS             30422..30715
                     /locus_tag="PLV81_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09585.1"
                     /translation="MNTSEQKSIVQYSTIVVILICFIIAFIYQNIEYMQLKMHYNAMI
                     KAYHTILEEYEREKYQLNKALSMEQIEIFAKAHGLEPMTKESLVILKSKQGTH"
     gene            30715..32241
                     /locus_tag="PLV81_05485"
     CDS             30715..32241
                     /locus_tag="PLV81_05485"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOM09586.1"
                     /translation="MDIRTYNSFDLYTLLNDTDVTIIKGDEDIRISAIEYDSRKVEKE
                     GLFVAIEGMETDGHKFIKDAVENKASCIVLQENRLDEFGFLLDQQDIAVATAKDTRRA
                     LSQISARFYGEPSLQAVVIGVTGTNGKTTTTYMLESILSRAGFTCGVLGTINYRWKDK
                     VINASHTTPESKDLQEIMSIMVLDGVDCIIMEVSSHALSLLRVDDIHFDCAIFTNLTR
                     DHLDYHITFNDYFMAKVRLFEILNKSLKQKKAAFINADDNYGKQILNWRERYGYPLFS
                     FGITNNTDYHCIESSIQISIKGTQFATDLPFAKQFTMKLAGRFNVYNALGAIGVADFL
                     NVPVGRIVTGISVLSSVPGRFDTILSPEGFAVVVDYAHTNDALEKLLLSVRSLKPERI
                     ITVFGCGGNRDKTKRPLMGKVSEDYSDWVIVTSDNPRKENPEAIIRDITTGMKTNRYQ
                     VIVNREEAIKHAIYMANQDDVIVIAGKGHEDYQIIGTKKYHFDDREVARKYINERLGK
                     "
     gene            32238..33614
                     /gene="murF"
                     /locus_tag="PLV81_05490"
     CDS             32238..33614
                     /gene="murF"
                     /locus_tag="PLV81_05490"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOM09587.1"
                     /translation="MIVRFETTSQTIATAINAIHHGSGQPVQYITTDSRLIEDKSLFV
                     PITGNTFDGHDFIEDVCKTGKCTSFLTHKQDKVNIADRYNTSAIYCNDTLQAYAAIAR
                     WHRKKFNIPVIGVTGTNGKTTTKEMIAQVLSTEGQCLKTQKNYNNEIGVPFTILHLQN
                     THSYAVIEMGMNHPGEISRLSQIAMPTIAVITNIGEGHLEFLGSVENVAHAKAEILDG
                     MAAGSICILNNDTKHAEIVKKKAREKNINVITFGLTGDIAPESYTLFQDKTVVEYDGV
                     EITVPAYGFHNVYAAIATIAVARIMGKSVIKVKEALSNFKPVAMRSDIKKGRCTIIDD
                     CYNANPLSMEYAFTSASMVFPDKRKIAVLSDMKELGTYSRDLHFTVGTRLKASGFSHV
                     LVWGNDARYIAEGAIAGGLDNSLVNVFGSKEELIAFLLQFVKHDDVVLVKGSRSMKME
                     EVVNALEQ"
     gene            33622..34707
                     /gene="mraY"
                     /locus_tag="PLV81_05495"
     CDS             33622..34707
                     /gene="mraY"
                     /locus_tag="PLV81_05495"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOM09588.1"
                     /translation="MFYHFILPLQEYLSFLRLFQYITFRSAYAALTSLLIVLIFGGLV
                     IRWLTHLRFKEEIRTLGPQSHQKKAGTPTMGGVLILGAVLISALLWGNFNNLYFILLI
                     VITVLLGAIGFIDDYIKSILKKKDGIPARLKFLLQCVSGLLIALCIYFFPSNAKEATT
                     LYVPFINRAILDLSWVWILFAMLVIVGSSNAVNLTDGLDGLAIGTVTIVVSVLAVMAY
                     LTGHQPIAQYLRIPYIPQAAELTVFLSALGGAGLGFLWFNSNPASVFMGDTGSLALGG
                     IIGTVAIMLKKEFFLVIVGGIFVIEALSVIVQVASYKLRKKRVFKMAPIHHHFELSGW
                     PEQKVVVRMWIIGIILAILGLSSLKIL"
     gene            34778..36157
                     /gene="murD"
                     /locus_tag="PLV81_05500"
     CDS             34778..36157
                     /gene="murD"
                     /locus_tag="PLV81_05500"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOM09589.1"
                     /translation="MEQKKHILVVGLGYRTGLASANFLVGKGFDVIVCDRKSHEELLP
                     VIQKLDQSVKVIAGNQDPSILDTGFNEIILSPGVPQSIPLIQEAKGKGIPVISEIELA
                     YRYCTSRIVGITGTDGKSTTTMLTAHCLKASGFDAHPCGNIGIPYVSIVDTLKKDSVA
                     VIELSSFQLETIDTFKPDVAILTNLTPDHLDRYNSLEEYFAAKMNIAKNQDSNDYFIV
                     NSDDEFIATHLPSLKATKFTFSLQKDADCYFKEGGIYCTLNGRQQLIAQASRLKILGL
                     HNVQNAMAAILATLSLAKKIGRDVNLKTLEDALYSFEGLPHRVQMIGSFEGRAFINDS
                     KATTVGAVIMALKSIPGDVILIIGGRAKGDDYSRLKNYLKGKVKALVLIGETKDEFAQ
                     LFNDIPHVKAHSMEDAVVKAMKVSGEGDTILLSPACASFDMYKNYEERGNDFIEIFKR
                     LKQGQLRWM"
     gene            36148..37305
                     /gene="ftsW"
                     /locus_tag="PLV81_05505"
     CDS             36148..37305
                     /gene="ftsW"
                     /locus_tag="PLV81_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOM09590.1"
                     /translation="MDVKAVIDTRRKGEPEMGLFIVAFILIGIGIAMTYSASALFAER
                     TFGDSLYFLKRQLLWSCIGFIIMMFVQQIDYRNYQQYTKIMLVFTLVTLVLVLIPGLG
                     ISVKGSSRWLGYGLLRFQPSELVKLFIVIYFAKVFSYEHQRDVNVLQLLLPLLIVGVF
                     FLLIMLQPDFGTAIDVAIISVVLLFVSGFPLMYMLGLAILSVPMFYLLIYQVEYRRER
                     ILAFLDPWSDRFGKGYHIIQSFIAFKLGGLLGAGLGSGTQKIKRLPEPHTDFIFAVIA
                     EEAGLIGTFSVVILYLLFFYFGVKIALRAPDEYGRLLAMGLTLLVTVQAFLNISVVIG
                     VLPTTGIPLPFVSYGGSSLVVTMIGTGILLNISKYREVVAQELKYSGEVWV"
     gene            37302..38414
                     /gene="murG"
                     /locus_tag="PLV81_05510"
     CDS             37302..38414
                     /gene="murG"
                     /locus_tag="PLV81_05510"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOM09591.1"
                     /translation="MKRTVLISGGGTGGHIMPGIALYEEFKSRGYNPILLVGVNDVKF
                     SSLNTIENLHTYNAPTMWKNIFNLPTFTMRFISALLWTMRFITKNNVAAVIGMGGYVS
                     APSLVGALLKKIPVYLCEQNVVPGMVTNLFAKRAKALFTTFEATADYCDIEIAHSIIH
                     TGNPVRKHVLKQVDKNIALKRFNMMHCKKVILVIGGSQGALKLNELMYDMLTQYPENF
                     TDTGIIWSTGSYSYEQFQKKLQNVAHRASIFLAPYILDVENSYAACDIAISRAGAGVL
                     MELAAWGIPSLLIPYPYAAKDHQKKNAIEFVKAGAAEMIENSEATAQKVAPLLFDLLD
                     NETLLSRMSAKAKSLARINAAQDIVNFIVKDCDLEK"
     gene            38426..>38731
                     /locus_tag="PLV81_05515"
     CDS             38426..>38731
                     /locus_tag="PLV81_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOM09592.1"
                     /translation="MFRKSKKMHFIGIGGIGMSGIAEILINLGYEVSGSDLRESEQTK
                     RLQKMGATIFIGHYPSNIKDYHVVVTSSAIHKNNPELIEAKNKKIPVIHRSEMLAELV
                     "
BASE COUNT        12023 a         6686 c         8238 g        11584 t
ORIGIN      
        1 tggttgtcac acgggaagag acgttcattg caacatcatc acggcaatat gcaaactccg
       61 tgtaagaagg tgcactggca tacagtgtca gagcaaaaaa agaaaagaag caagaaaaaa
      121 tggaaaggtt taatcatcgg taactatcgc ccatgggtga cagagccatt ctgcatagta
      181 aattttatta ttataaatga attaaaaatt aggtgaaatg cttgacaaaa atttatgtat
      241 aagttaatgg ttattcatta acttatacat aatattttag ctgattttat tggaggcatt
      301 atgggtgtca gagcaattaa aaaagtaact gttattggct caggtgctat gggtagtggt
      361 attgctgaag tacacatcgt taatggattt aacacagtaa tggttgatat caacaatgat
      421 ttgttacaga aggccaaaga aaaaattaag tcaaaccttt cttttctggt taataaacag
      481 aagatgacac aagtagctat ggatgaagca atggcaaggc ttgctacttc tactgatatt
      541 acatcgtcag tgaaggatgc tgattgcatt attgaagcag tgccagagct catggattta
      601 aagaaaaaaa tattcaaaac agtatctgat gcagccagga cggatgcaat tattgccagt
      661 aatacttctt cgctgagtat tagcgaattg gctgaaacgg tgtcagagcc ttcacgattt
      721 gctggtttcc atttctttaa ccctgttaat agaatgcgct tagttgaggt tatttatggt
      781 cagaagacat cagatgaagt catcgataca ctcatggatt taggtgaaaa gttgggtaaa
      841 gtggctatta aggttttacg tgataggcct ggatttattg taaacaggat aaatgctccg
      901 ctacaaactt tgtggagtgc aattctggat gaaggaaaaa tttctccagc aagtattgac
      961 actgtaatga aaaatcacgg agcaaagatg ggcccttttg agcttatgga ttttgttggt
     1021 ctggatgtta tttataatgt tatgcaatat tataaaacaa cactgtcacc tgaatgggag
     1081 ccaggaaaat ttataactga atgtgtaaag aaaaacgaat tgggcatgaa aaccggcaag
     1141 ggcatttatg tatgggaagg cggtaaagca attattgatt catcaatagt aacagacatc
     1201 ataaagccaa ttgacccatt agctgttcag cttaatgaag ctgtccgtgt tcttaaggaa
     1261 aaagtagctg caagtgctga agatattgat aaagggcagg aagctggcat gaatcagcca
     1321 gggccgttca aaacagcaat gagtattgat aataagcttt tagctgaacg gctgaactgg
     1381 atgagtaaaa catataatct gacatatctt aaacctgaac ctgaaatact ggatggtagc
     1441 tttaaatcat ttatgagata gagggaaggt atgagtaaga acgaatgcat tatcactgaa
     1501 gtgaaagatt cagttgggct tgtgtatatt gatagaactg aagtcatgaa tgctcttaat
     1561 agaacgttaa ttgtggagtt aggtaacaag attaaggata tgtgtgctga tgctggtgtc
     1621 agagccatca tcatttctgg taaaggcgat cactttgcag caggtgctga tattggtgaa
     1681 atggcacagc aaaaccccca ccaggcattg gctttcagtt ttaatgatgt gtataccatg
     1741 attgaacaac ttcctgttcc cacgtgtgct gccattaaag gatatgcgct gggtgggggt
     1801 ctggaattgg cattagcatg tgatttcaga atttgccata catcagcaaa gttaggcctt
     1861 cccgaaataa agcttggcat tattcctgga gctggcggta cacaacggct tccacgtctt
     1921 attggtttga gccgtgcaaa agaaataatc tttagcggtg aatttatcac agcagaaaaa
     1981 gcttaccagt ggggattgtg tgatcgttta acggataatg atccagtact tgaggcagta
     2041 agcttcctca gtactattat caaacgtcct tctttagcat tacaggcagc aaaaaaatca
     2101 ataatgtttg gtctggatag ctctttgaaa gaagggcttg aatatgaagc attatcgttt
     2161 ggtattttat tctccaccca tgatcagaaa gaaggaatga aagcttttat agaaaaacgc
     2221 aaacccaatt ttaagggatg ttaggaggcg ttatatggat aataaagatg ttgttatagt
     2281 ttctgcatgc agacttgcga taggaaagtt tggaggtatg tatgctaata ccagtgcact
     2341 ggagttaaca attcctgtta tgcaggcgct cgttcaacgt tcaggaatag atcccgagct
     2401 aattgatgat tgtatctggg gttgtaatta ccagaggact aatggtgaaa ataatttagc
     2461 ccgtgttgcg ctcgtaaaag caggtcttcc tattactgtg cctggtatta ctgttcatag
     2521 aaattgtacc tcttcaatgt cttcggtgca attgggatat tatcaaataa aagctgggga
     2581 agccgattgc ataattgcag gtggcactga tagtatgagt aatgcacaat acactattga
     2641 taaaatgcgg tatggaactc gtataggaca tactgaaata cgtgattcaa tgtgggattc
     2701 gcttacaaat ttaggtattg gtcctgcaat gggcataacc gctgagaatt tagctacaaa
     2761 atataatatt tctcgtaagg agcaggatga attagcattg ctatctcata aaagagctat
     2821 tgcggcaatt gataagggat attttaaaaa ggagatagtt cctgttgaag tcaaaggaca
     2881 aaagggaaca atcattgcag atactgatga acatccacgc cgcgatgtta ccatagaaaa
     2941 actggcagca ttaaaaccag cgtttaaaga tgatggaact gtcacagcag ggaatgcttc
     3001 aggcataaat gatggtgctg ctggtgttat tttgatgtca gcaaaaaagg ccagagaagt
     3061 aaaggccccc attataggaa ggattatatc aacagcaacg gcaggagttg atcctgatat
     3121 tatgggtatt ggtccagttc cagcaagcag gaaagcactg gaaaaagcaa gactaacgtt
     3181 aaaggacata cagcttgttg aaataaatga agcgtttgct gcacagtacc tggcctgcga
     3241 aaaagaactg ggcttaaaca gggaaattac aaatgtaaac ggtagcggta tttcattagg
     3301 ccatcctgtt ggtgctacag gcgtacgact tatagtaaca cttctacatg aaatgaaacg
     3361 gagaaatgtc cagtatggcc ttgcaactct ctgtgcaggt ggtggtatgg ggacagctgt
     3421 tgttatagaa catatataaa gcacaaaggg ggaatcggta tgcgaccaaa tcttttagta
     3481 gattcacgtg acgttagatt tgttttattt gaaatgtttg atcttgtatc actatcagaa
     3541 tatgaaccat ataaagaatt tgatagacag acatatgaag ctgtccttga tctggcagaa
     3601 caaattgcca tggaagaggt ttaccctgcc aatactatag ctgacaaaca aggggtaacg
     3661 tttaatcccg atactcacac tgttactgtg cctgaagtat ttaaacaggc aatgaaagta
     3721 tttaacgaag ctggattcac aggacttgtg cagccaattt cttgtggtgg tatgggtatg
     3781 cctgagatga tgtatcgatc agcacttgag tattttctag ccgcaagtat ttcatttacc
     3841 atttatatta cactgtcaaa cggtgcaacc aatctggtgc gcatatttgg taatgaagaa
     3901 caaaaacaat tgtatcttca gaatatggta actggtaggt ggggtggtac catgtgttta
     3961 actgagccac aagcaggttc agatgttgga gctattaaaa caaaagcata caggcaacca
     4021 gatggaacat ataaaataaa aggacagaag atatttattt catctggtga aaacgatttg
     4081 tatgaaaata taatacacat ggttcttgct cgtattgatg gtgatccaga agggacaaaa
     4141 ggattgtccc tatttcttgt tccaaagatt ctggtacata gcgatggttc gttgggtaat
     4201 cgcaatgacg tcatatgcac tggtgtggaa cataagatgg gtattaaagc ttcagcaacc
     4261 tgtacgctaa gctttggcga taatgataac tgtattggat atattttagg caaagaacgt
     4321 gatggcatca agaacatgtt ccacatgatg aatgaagcac ggcttgatgt gggattggag
     4381 ggattaggtg tttccagtgc agcatatatg catgcagtcc agtatgcaaa atcacgtatt
     4441 cagggaaaaa caatttcaaa aaatgagcct tcaattatag gacaccctga tgttaagcga
     4501 atgctgttat ggatgaaatc acatgttgag gctatgcgaa tgcttgtagc tcttattgca
     4561 tatcatattg atatttcacg agtacgtaat gatgaaattg gtaaaaaggc acaggcactg
     4621 gtggaattct taatccccat ctgtaaggcg ggtaataccg acctatcatg gcttattaca
     4681 gctgaggctg ttcaggtata tggtggctat ggatattgtg gtgagtatcc tgttgaacaa
     4741 tatgcccgtg attccaaaat tctttctatc tatgaaggta ctaatggcat tcaatcaata
     4801 gacctgacca tgcgtaaaat tttgtctgat gaaaagcgcg agcggtacaa tgcatataaa
     4861 gactatgcca gtgatgttat aaaaaaagcc cgggcgatag ttcctgaaaa tattattaac
     4921 caggtaacaa tcgctattgc tgaaatggat tccctcattg atgtgcttgc agcaaaggat
     4981 acagcttttg cgttgagtgt tgcaacaatg ttgcaacaag ccttcagaat acttacacat
     5041 ggaatactgc acctgaattc aatgaccata gcaatccaga agttgcaatc gctggcaggt
     5101 ggctatacat acaatcaagt tccagaaggg ttagaagata attccgaaat agctttttat
     5161 tatggcaaag tactatcagc acaatttttc cttacagtgg aatttgaaaa atattcaggg
     5221 attatacagg gtatccgcaa tgaaaacgga gttgtattgc gggctggttc atcaatcttt
     5281 actggtgttc ttgaagcgta aaacaatgca aattcatccg tccatttaca tccataatgc
     5341 cgatataacg ccgcgcggta aaaaagcact cattgttgag ggtgggggca tgcgcggcgt
     5401 tttttctgcg ggagtgcttt ttgcaatggg attgcgtggc ctaactcatt ttgatttgta
     5461 tattggtgtg tctgcaggtg catgtaatct agcatccttc cttggcaggc agtatgagcg
     5521 taacttctat ataatggaaa actggtcagc atcatggagg tttataaatc ctttcaggat
     5581 tttctgggga aatgttttgg atcttgactg gctatgggac attactatca gtaactatcg
     5641 cctaaattta catgaaatat ttaaggatag tagtagagag tttcttgttg ttgtaacttc
     5701 aatggaatca gggaaagcgt tatatataaa accaaataaa gaaaatctgg aatttgtgtt
     5761 aaaagcttca agtgcattac cagtattata tagaaataaa ttatttatag aaggtcaaag
     5821 agttgcagat ggtggtgttg gtgattcaat tccagtcaag gaagcataca aacgtggtgc
     5881 acgcgatatt gtggtaatac gttcacaggc taaagaatac aggaaagaca aatctgaaag
     5941 cggtacttta ctgaaattat tgttcaggag ctatcctgaa tttgttaaag ctttagtcca
     6001 aaggccagta aattataata aggcaattga atttattgaa aatcctcctg acgatacacg
     6061 cataacagaa atatgcccac ctgcctggtt taaaactaaa cggacaacca caagcaaaca
     6121 acagctttta aaggattact atagtggcat acagactggc tttttgctgt gtaattatta
     6181 taaatcttta caataaaaat ggaggacttt tatggagcgt ttatgggaaa agaattatga
     6241 tttctggtac cattctgtta actttcagag gatgacagta caggatatgt tgcattgtag
     6301 tgccgccgtt gcacctgata aagttgcaac ggatttcttt ggcacacaaa ttacctatta
     6361 ccagttgcgg ctgttatcaa taaaatttgc acaggcactg gtaaaaattg gcgttaaaaa
     6421 agggggtaga gtttgtttgc aattaccaaa ttcaccgcaa tttgctattg cttattttgg
     6481 cacacttatg gccggtggaa ttgctacatt aatgaatcct ctatatacag cagatgaagt
     6541 taatcaatta atttccatta cacaaccaaa tgcatttatt acatttgata tgatattgcc
     6601 aaaccaaagg gatgtgttaa agcaatctgg tgtaacagtc atagtaacca aaatcactga
     6661 ttttatagat ggatttccaa aaggcagtgc tcaagaactt caacttgaaa agaactggca
     6721 ttatttttct gatttactgg acagtattga tgaagcttct gttcctaaga ttgatataag
     6781 tcctgatgat ccagctataa ttgtttttac tggtgggaca actggtgctc ctaaaggtgc
     6841 ggtacattct cattacaatg ttgttggtgc tactatatct actatggaag ttggaaaata
     6901 ttttattgat ttaatgccag ctgaaacacg gtgcaatatt ggtgtaatgc cgttttttca
     6961 tattgcaggc catattcagt gtttcacctg gggtattttc cgacagacaa caatgtacct
     7021 ggtgccaagg tttgatataa atgaatttat taatttgctg gcaagaattc cccataaaat
     7081 cacttatttc tttgcggtgc caacattgct tacagcactt gtaaatcatc ctaaagctaa
     7141 ggagcttgcg cttgataaaa agtttggact tatactatcg ggtggtgctt catgtcctgt
     7201 gccacttatc aataagatga aagatatggg attattttat attgaggcat ggggaatgac
     7261 tgagacatgt ggtattggta taagtacacc aatatttggt ataaaaaagc ctggtagcat
     7321 aggaattcct atgcaggaat caatgataaa gattgttgat ttagaagatg gaaaaactga
     7381 agtaccaatg ggaaaaccag gtgagttaat atttaaaagc ccatttataa tgcaagaata
     7441 ctggaataat aaagaggaaa ctgctcatgt actaaaagat ggatggctgt acacaggaga
     7501 tgttgtgact atggatgagg atggatttat ctacatcgtt gatcggaaaa aagatatgat
     7561 cattgcaggc gggtacaacg tatatccatc tgagattgat gatgttcttt taaagcatcc
     7621 aaaggtggat aaagctgtta cggttggtgt tccagataca tacaggggcg agacagtaaa
     7681 agcatttatt gttttaaaag agggagaagt tgcaacagaa ggagaaatta tagaatattg
     7741 caaacagcat ttagctgcat acaaggtacc aaagattatt gagttcagaa aagaattacc
     7801 aatgtcatct gttgggaaga ttttgcgtaa agtgcttcgt gaagaagaaa taaaaaaagt
     7861 tacaaagtaa aatcaacaag atattaagag cgattaaaag agtcataata aaccccacct
     7921 ggcagagtgg ggtttattat ataatattta taaggttatg gcgttgcaaa taattgtatt
     7981 attcattgtt taatactata gccaattata aagatggcgt tatcaattgt attaacacag
     8041 ttactcttct tgaggcaatg aagccaattc gggataacgt gcgtgtactt gtgtgtaaaa
     8101 atctctgatt gttttgaagt cagtttttat atctccggtg ggatgtagta tggcacctat
     8161 gcccccagat tttgttttgt aatctaaaaa tgccagaatt attggaatat gtgcaccagt
     8221 tgcaatatgg taaaaacctg ttttccattt ttccactttt tttcgagtac cttcaggagc
     8281 taatgctatt gcaaattttt catgcttatt atacatatca attgttgcct gcacagtatt
     8341 acttgatacg gagcgatcta taggtatgcc acctaaaaaa cgaaccagcg gtgcaaacgg
     8401 gaatcggaaa agttgatttt ttgccatgaa atagatatca acattaaacc caaaggctat
     8461 cagaataccg taaaatacat cccagttgga agtatggaat gcaagtatgg ccaaatattt
     8521 gggaaaatcc ggaagccttc ccactttttt ccaacctagc aatgttctta acataaatgc
     8581 aagagcacga agtatatgtt taactatggg ggtattgaac attgtatatt gaatacgctt
     8641 tgttttcatg gtaactatcg ccctgaaaaa attttggatg attcaatcat atggctaatt
     8701 atatgacaat aatattttat gaggttgcaa aaaattttgt agggtcagag ccaaagataa
     8761 atgctcattt gtcattgaca tcagaatggg gctatatata atcaaaaaat ggggtcagag
     8821 caatgaatat gcattggaat tgcagtgttg aggaagctat tgctatacaa aaagagctag
     8881 ctggtaacgt tatcattgcg ggaaagcctg aaaaaattga tcatattgca ggaattgaca
     8941 tagctctgac cccggataac aaggggtatt gcatcattgc aatcttcagt ttccccgata
     9001 tggaactttg cgaggaggtt tttagctctg acaccataaa ctttccgtat gttcccgggc
     9061 ttctatcttt cagagaaggg ccattagtgt tgaaagctta tgaaaagtta tctataaaac
     9121 ctgatgttat ggtttttgac gggcagggta ttgcgcatcc ccgccgttgt ggcatagcca
     9181 gccatattgg tgtgttgctt gatattccat ctataggctg tgcaaaatca cgattgtgcg
     9241 gtacatacat tgaaccagga caagatagag gtgccagagc atatttgtat gataaagagg
     9301 agatcattgg tgtggtgctt cgcactcgtg cacatgttaa gccggtattt atatcgccgg
     9361 ggcacaagat tgggatagat gaagcagccg atatcatgat gcagtgtacc ggtaactatc
     9421 gtatacctgt acccacacgc tatgcgcact gcagggttac tgagtataaa agatcaggag
     9481 tagatactgg cttaaaatca tattaatttt ttaactcagt tgatgtaatg taaaaattta
     9541 taacataatg gtggttctat atgcatccca tttcaggtac taactttact gtttatatcc
     9601 atgatcttat ccattcatgg cttaaaactc tgatactgct tggttctatt ttagttcctg
     9661 gttttttgat acttgattat gtaattacgc cacatgattt gttccctcgt tttgttgtgt
     9721 atcgatgtgt atcaacagca ttattaatta tacagtatgt catacttcga atttcaaaac
     9781 ctggcagatt cagttttatt catggttatc tggcagcatt aaatacaggc tttgttattg
     9841 cacttatgac agtagattta ggtggatttt catcagggta ttatgcaggg ctcaatctgg
     9901 taattattgg tgtaaattta ttaacgccct ggccgtttat tcattcgtta attaacggat
     9961 tggctgtggt gtgtatgtat gtagggctta atgttgtttc gtcacaatca actgactata
    10021 tctacatgat taataatctt ttttttatgg tatcaactgt cgttattaca gcaagcttta
    10081 gctttttacg gtttaaacaa ttaaaaagtg aatttgattt aaccacaata attttattga
    10141 cgaatagaag tgtacaattt acactttaaa aaaattattt ttccattctc tgtatatact
    10201 ctactatttc atgattatcg tggtatgttt ttagtgaata agcatgaaag agttatcgta
    10261 taattttttt aatgtgagaa tcatcaatgg aacaaaaaaa gataattatt gccggattgg
    10321 gttcagcagg ttttgcagca ctggtaacaa tcaagcggat taatcctaaa gcccatgttg
    10381 ttgtgataga ccccaaagac aaagatattg tccatccctg tggattgcca tatgcgcttg
    10441 aaggcaaagt caatgagcag gatttacagc aggatatatt tctttccaga atgaatgtgg
    10501 aaaaagtaaa gggtattgta cacagcgtag atgctgaaaa aaaagaaata gctatccaat
    10561 acgatgataa aacgatcacc ttgcagttcg atacgattat actgtgtacc ggctataaac
    10621 ctcagttgcc accaattgct ggtattgaac actatataca caaaggggta tatacgttaa
    10681 gcagcattga tgatttgaag agaataaaaa atgacataca ggggaagggt agggcgatag
    10741 ttatcggtgc aggggcaata ggcctggaag ttgcctgtgg attgaaagcg catggcctta
    10801 cagtaaccgt tattgaagca aaagaggtat tgccacaggc agttgacagc gatatggccc
    10861 agctggtagc actgtactgt gccaatcaga atattgaaat tataacacat tcaccggtca
    10921 cggcaataga aaagcgcgat agtgaattta gtattagaac accggaaaaa gaatatactg
    10981 ctgacattgt cattgttgca accggttttg ctgctaactg cgaatgggca gttaattcat
    11041 catttgaaac aggaaaatgg ggagttgttg tcaatgaaca tttagaaata aaaccatcaa
    11101 tatatgcagc aggggattgt atacagaact ggtcggtcat tgataataaa ccttttgcgg
    11161 ttaaattagc aacatcagca tataagcagg gaatcgttgc tgcagagcat gctatggggt
    11221 tgaattcagc ctataaagga actgcaggta cgtttgtaac aaaggttggt gagcttgaaa
    11281 tagctgcaac aggattttcc acacaggttg cccaggaaag aggtttttct gtgatagcag
    11341 gaaaaattag tgctacagta aaaccagaat attttcctga aaatgaacgt ttgactttca
    11401 aggtactgtg tgatacaaaa ggaaaactat taggtgccca ggcaattggc aatggtgccg
    11461 cagcacgaat aaatataata agcctggcta tagaacatgg attaaccatt gatgacatac
    11521 tgccctttga aatggcatac tgtcctgctg taagcgaggt taatgatatt ttattcaaag
    11581 catgtgaatt tgcaaaacgg agaatcaaat gacagcacag agaacgtttc tatatgatga
    11641 tcacataaag ttaggtggta agatggttga atttgctgga tgggaacttc cggtaatgta
    11701 ttcttccatc attgatgaac atatggcagt gcgccaggct gctggtttat ttgatgtttc
    11761 ccatatgggg gagatagttg ttaaaggaag aggtgcgcgg gcagcattgc aaaagcttat
    11821 tcccaccgat atgggaaggc ttgaaaaagg taaatcaatg tacacctgct ttctaaatga
    11881 taagggtgga attatagatg atctctttat tttcatgctt tctgataatg aatattacct
    11941 tgtggtcaat gctgcaacca cccaaaagga tgtagagtgg ttaacacagc attgtaaaac
    12001 taatgtgtca atacaggatg tttcagcagc aacggcaaaa attgatctgc agggcccgca
    12061 atcatataat atcttgaaaa aaataatcag agatgaccgc ctggaaaacc tacagcgatt
    12121 ccatttcttt accggtgctt ataagggtga gccggtgctt atatcccaga caggatacac
    12181 cggcgagttt ggctatgagc tattctgcat taataatgtt gcttcccggt tatggaatga
    12241 tgtgcttgaa gcaggcaaag aatatggcat aaaaccagca ggccttgggg cacgggatac
    12301 attgcgttta gaggcttgct attcgttgta tggccatgaa ttgagcgata ccataacacc
    12361 tgttgaaggt ggcattggtt ttgtggtgag tgcacaatct gaatatattg ccagagatat
    12421 tgttgaatat caaaaagtac atggcgcgcc acggcagata atctgtttag agatgcttga
    12481 taaagcaata cctagaaatg aatacagagt gctgtataat aatcaggata ttggatatat
    12541 tacaagcggg ggtttttcac ctgtttttaa aaaaggcgta gccatggcgc tggtaacaag
    12601 tggtgttgtt tcagtagggg atgaggtggc gatagttatc agggataaaa caagtcctgc
    12661 aaaagtagcc cagcgcccat tttacaaata ttcaggtaaa taattataac gtcattttga
    12721 agagcgaggt ttctattctg ttatttcgat gaacgctttt tacattgtca tttcgacaaa
    12781 cgcttttata tattgtcatt tcaacgaacg cttttttaca ttgtcatttc gacgaacgaa
    12841 gtgaggagaa atcttaatcc tcagagaaga aagatttctc atcccgatga aatcgggctt
    12901 cgaaatgaca gtgtgannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnattg
    13021 tcatttcgac gaacgaagtg aggagaaatc ttaatcctca gagaagaaag atttctcacc
    13081 ccgatgcaat cgggattcga aatgacagta taaggaataa gggattcaaa atgacagtgt
    13141 aaataacaaa ggatttgaaa tgacaatata aacaataaag aatatgaaat aataatttaa
    13201 taattattgt tacattcaac tttttcaagg agcacaacaa tgagcaatat tccagaaaat
    13261 ttaaagtaca caaagacaca tgaatggatt gatgtgcagg atgattacac cggtatttgc
    13321 ggtattaccg atcatgcaca ggaaatgctt actgatattg tatttgtaga attacctgag
    13381 gtagggattg aggtcaagag tggtgaacag gttgcagtag ttgagtcagt caaagcggtg
    13441 agtgatgtat atgcaccagt aagcggaagg atagttgaag taaacaaaac gcttgaagat
    13501 tcaccagatt tactgaacag cgacccctat ggcaaaggct ggatttttaa gatagatata
    13561 aaagataaac atgagcttga tgaacttatg gatgcagcgc agtatgctga gcatgttgct
    13621 tcaggagaat agacatgagc tttttaccca acagcgatga tgatataagg tcgatgtgca
    13681 atgagattgg tgtcaattct gtagaggacc tttttgttga tataccatct acggtaaaaa
    13741 atccagacat ccaattacca tcaccgttat ccgaacagga agcaatgcat tctttacttc
    13801 gcattgccaa tactaataaa cgggtgtgtt cacttgtagg tggtggcata tatcatcatt
    13861 atgtgcctgc ggttgtggat cacctatcat cgcgatccga attttacaca gcgtataccc
    13921 cctatcagcc tgaagtaagc cagggaacac tggcggcaat atttgaattc caaacgtata
    13981 tgtgcaggct tacgggcatg gatattgcca atgcaagcat gtatgatggc gcaacagcgt
    14041 tggctgaagc ggttctcatg gctgtgcgta caaccaggaa aaacaacgtt ttatgttcgg
    14101 ctgcagttca tccccattat cgtcaggtgc ttgctacgta tgcttgggca tcggatattt
    14161 cattacagat agttccgtat aaaaacggtg caactgatta taatgaacta tcgcacataa
    14221 taaacgatac tgttgcctgc gttgttgtgc aaagtcccaa cttctttggc gtgattgaag
    14281 acattgaaaa agtagcacac attgcacatg agcacaaagc attatgcatt gcaacattta
    14341 ctgaagcaat gagtttaggg ctattaaaaa gccctggtga atgtggtgcc gatattgcat
    14401 gcggcgaagg gcagtcgttt ggcaattacc ctgccaatgg agggcctttg ttagggatac
    14461 ttgcttgcaa aaaagaattt atgcgaacca ttcctggaag gcttattggc aaaacggttg
    14521 atgaggatgg tagggaatgt tatgtgatga cactgcaaac acgagaacag catatacgca
    14581 gggaacgcgc cacatcaaat atatgtacca atgaaggatt gtgtgccttg cgggcaacaa
    14641 tataccttag cctggtgggt aacaggttag tagagctagc tcgtacaaac catgctgcag
    14701 caagctattg cctggaacaa ttagtcaatc ttggctatga gcgtgtgttt gatgcgccgt
    14761 tttttaatga atttgtcatt aagattaaca atgcaaaaaa tgttcaaaac aagcttgtgg
    14821 ataatggatt tgcattaggg ataccgcttg aaacatacta tcctgagcta tctgattgcc
    14881 ttttgattac agcaactgaa tgtactacta aagatgatat agacacactt gtaaaactat
    14941 tgcagacaat ataatggtga gtaccatgca aacaatattt tcattacata aacaaggcag
    15001 gaataaatat actgtaccgc aatttggtgc agaaagctat gatcctatcc cacaacacct
    15061 atgccgtggt gagcttaacc ttgctgatgt gagcgaagtt gatgtcgttc gccattaccg
    15121 taggctttca agcttaaatt ttggtgttga taatggcatg tatccgttgg gaagctgcac
    15181 catgaaatat aatccaaaga taaacgatgc agttgcaagt ttacatcagt ttactcagca
    15241 acacccggca actattgaag agctatcaca gggatcgtta catataatgt attccttgca
    15301 ggagatgcta tgtgcaataa caggaatgaa acagtttacc cttatgcctg cagctggtgc
    15361 acatggagag cttacaagtg ttatgattat aaagaaatat tttgaaacaa aaggtgaaaa
    15421 acggaatatc attctcatcc ctgattcagc acatggtaca aatcctgcat cagttgccat
    15481 gtgtggtttc actgtaaaag aaattccttc caacagcgat ggtgatgtcg atatagatgc
    15541 actgcgtgct gcaatggaca acaatgttgc agctatgatg ctgacctgtc ccaatacctt
    15601 agggcttttt gatagaaata ttatgacaat tgcagatata ttacacagta atggtgcatt
    15661 gttttactgt gatggtgcaa acttaaatgc aattgttggc aaagtgcgcc ctgcagattt
    15721 aggttttgat atcatgcatg ttaatttgca caagaccttt tcaacacccc atggtggtgg
    15781 cggaccaggt tcagggccaa ttggtgttgt tgaaaaactt actcccttta tgccagtgcc
    15841 tgtgattgca aagaaggata atcagtttta tcttgattat gaaaggccac aatcaattgg
    15901 gcgagtgcat agctattatg gaaatttttt ggtaatgctt agagcctata cgtatatagt
    15961 gatgttagga gcaagtggct taaaacaggt ggctgaacat gccgttgtca atgcaaacta
    16021 cctgcgtgtt aaattgcaaa agcattttaa tctgccgtat acacgtatat gtatgcatga
    16081 gtttgtcata aacgacgaag gtatgccaaa tggtataaca acaaatgaaa ttgccaagcg
    16141 ccttcttgat tatgggttcc atgcaccaac ggtttacttc ccgctcattg ttcatggtgc
    16201 aatgatgatt gaaccaacgg aaactgaaca cagggatacg ctcgatgcat ttgtggatac
    16261 catgattgca ataaaaaatg aagccaacac aaatccagaa gtgctgaaag ctgcaccgca
    16321 cacaacgcca gtgcgcaaag ttgatgcagt gcttgcagcg cgcaaaccgg tactgaaatg
    16381 ggaagagtaa ggaccatacc taactggata aaattttcaa taccttcagg tgagcagtat
    16441 aaacaggtgc aaaaacatgt agcaggctgt ggtctgcata ctgtgtgcct tgaggcacgc
    16501 tgtcccaata ttggcgagtg cttttctaaa gggcatgcaa catttctcat catgggcaac
    16561 atctgcacgc gcaactgtaa atattgtgcc gttaccaaag ggcaaccaca accactggat
    16621 atacatgagc ctcaaaacat agccagagct gtggcaaacc tgaatttaga gtatgtggtt
    16681 attacatcgg tgacgcgtga tgaccttgag gatggcggag catttcattt tgtaaaaaca
    16741 atagaagcca tacgcactgc tattcctgac acaaaaattg aggtattaat ccctgatttt
    16801 aaattttctc agcaacgttc acttactatg ataatggatg ctcaccctga tgtcattaat
    16861 cacaacattg aagtagtaaa aagctattac catacattac gtccaaaagg gaattatcag
    16921 gcttcgctta ctgttttggc gatagttacc gagcatggat ttattgccaa gtcaggtctt
    16981 atgattggat ttggggaaac catggatgac attatcaata cgcttgagga cgtatattcc
    17041 gccgggtgca gggtactgac ggttgggcag tactgtgctt cgcacagggg ggcatatcca
    17101 gtacataaat attatacacc agaagaattc aaagaaattg aaaccattgc ctatcagata
    17161 ggttttatga aagtacttgc agcaccgctt gtacgcagtt catacagagc aggtgagctt
    17221 ttttcatgag cattcatgtt gtactcaaaa atcctggtga aaaattagtc catattgata
    17281 ttgattttga aaagagtact atttcatgta ttcacatttc aggtgatttt tttatatatc
    17341 ctgaagaagc aattgtcgat atagaacaag ctttgttagg cttgccagtt gacccacagt
    17401 taattgaaca aactattttt acactcatta aaaataatga tatagaaatg gttggaattt
    17461 cggtttccac aattgtacag ggcatacagg aggcttttgc acaatcatga ctacctggcg
    17521 cctgattatt cacaatacat gtgcaccatc atggaatatg gcagtagata gtgccatatt
    17581 gaggcttaca aaaataccca cgcttaggct gtaccggtgg agtaagcctg caattactat
    17641 agggtatttt caggatatag aaaaagaggt acatatagaa ctttgcaaaa aagatgatat
    17701 tccggttatt cgcaggctta ccggtggtgg agcagtgttt catcatgaag agcttaccta
    17761 tagtatggtg caccctttac aaggcccatt tggcaaaagt agtattaccc attcttatgc
    17821 attgattgca cagccactga ttcatgcact gcgtagtatg ggagttgatg cacagtatcg
    17881 ccccatcaat gacattattg ttggcaatag aaaaatctca ggcaatgcac aaacccgaaa
    17941 agaggggaaa ttgttgcaac atggcaccat ccttgtggca acagatacag aaaagatgct
    18001 acagtattta actatcgatc ctaaaaaaat aaaacaggaa gggaatcccg ttatcacgtt
    18061 gagtgaaatc ataaaaggtt tgtcaaaaga aagtatgttt gagcaattaa ttaaggctgt
    18121 catttcttcg tttaaggaac tttttgcagt tgattttgaa gaagctgata ttacactgca
    18181 ggaaaacgat atggcaattg aattagaacg cagctatttt tccaatgaac agtggaattg
    18241 caggcgccta tcgccgtaac attacacatc aatgcccata tattttttaa taaaaaagcc
    18301 aatagcaaac gatatacatg ctacgccaag acttattatc gccatttcag aaaaacgttt
    18361 tttgaaatta aggtcttttg caatggcaat gtagtaatta aagaaaaaga ttatcagcag
    18421 cgcaattacc agcgtaatgc caagagcaac caggtaatgg cctgccacaa agtagggaat
    18481 aattaaaagc accaccgtga tgatataggc aattcctgtg tagatggctg actttaacgc
    18541 atcttttgca tcttcagctt tgcttgaaag gtattctgat gctgccatgg ataacgctgc
    18601 tgcaattccg gttataagac ctgctaaggc aataagatgt gtatttgcca gggcaaatga
    18661 aagaccagct aatgtgccag ttaattctac cagagcatcg ttaagcccca gtactactga
    18721 accaacatac tgaagatgtt cttcttcaag caagatatat aattcttttt cgtgggcatg
    18781 ttcgtctttg atgatctttt ttacttcggg gataatgggt tctaatgatt tataagaagc
    18841 ttgagcctgt tcttcgccgc gttccattaa ttttatgcca aatgtcagac caaataaacg
    18901 ggagataata taatatttta ttatcttcca ccgatcaggt ttaacatcgg aatgggtgta
    18961 gcttttccag aactcataat gctttttttc atcttctgct acttttttca atatacgtgc
    19021 attatgatga ttcttaatat gtgatgctaa aaggttatag atatggtgtt cggttaattc
    19081 acttttctga aatttaatga tcattaatat ggtttcagga gtcaaatgct tttgcataag
    19141 ttttccctac atctcaatga tatcatacac tatattaaat gagataagac attggaggac
    19201 agccttaagc ggcattcctt ttgtaaattt taccagaaag tcatttgaaa catcacaaaa
    19261 ctcccagctg gtgccttcta ccaggatctt gcctgagtat tccagtgaat ctgcatcata
    19321 caaaattact tctttgatgt tcatactcat actcaaacac tcctgaaagt aaaattatac
    19381 actatcattc tgaactaaac tcagaatctc ctgcacccca ccatcatcca aagatcctga
    19441 atcaagttca ggatgacgaa gtgtgtatgt cattcttcgt cccgattgca ttgggacgaa
    19501 gaatccaagt tttatacttc gagattcttc tcccagcatt attggagtca gaattaccag
    19561 ataaatattc agaatgttat gtgacagact acttctggac tattcaaata aaatcatcat
    19621 aattatcaag tgtttttatg tttccgcgtt gttttctttc aattatttct tttagtgctg
    19681 tcctggcttc atcgcttata aatgggtttt catttataat tgctgatcgt acacagtcaa
    19741 tggatttttc ggtgccaata tgttttaatc cctgtatagc cagcatcttg caatcctcat
    19801 catctatagt cagagcctgt tgcaacagcg tttctgattc ttctgatggg aaatatgaag
    19861 ccagctgtat aaggcgtttt tttacaataa gtggggcatc atgaaaagag gtaagcaaac
    19921 tgtgtgctgt attgttatca atactttttg aaagggcata aattgcactt gatttaataa
    19981 tggggtaatt ctcattcagt attaaatcgg tcaatggttt gataattaat ctgttgctga
    20041 atgcacccag tgcccgtgca catgcttcca cgaattgggg gttaggatca gaaagaccct
    20101 gaataagatg ttcaattgca cgtttatcct caataactcc taaggatcgt gctgcaaaaa
    20161 tacggaggct gaggctatgc gattgcatca tttctatgag ttttggcaca gataactttt
    20221 tgtatcgtat gaacagtggc atcatctcat tgatggggaa aatggtttca tcatttatct
    20281 tttcaaaaag tggttcaata gtcaactcgc caatattaat taaagcatct atggttatga
    20341 taattatctg tctgttgctg tgattgacaa acggtatgag gtattgaatg gcatcaggtt
    20401 cgcgtaactt acctaataat tggataatgt atgatagctg ctgaatgtca cgtgtggttg
    20461 ttagaagatt aattaacctg tcaagttgag gtgaagcaat tttttcaagg atgtatatta
    20521 tatcatcaat gttgcgggat gattgcaata attgttgcaa aagtgcttca aacgcaggct
    20581 caccaatatg ttctaaaatc caccttgcag tatccttgac caggatactg tcatcgtaca
    20641 aacaattaac aatttcctgt acagtttgtg gatcatcata gccaattttt accagtgacc
    20701 atgctgcggt ttctctaacc ttttcgtttt catcttttaa aagagggatc aatgcttttg
    20761 ttgcatgtgg gtttttgcaa atacctaaac agaaagctgc ggattcacgt ataacgggat
    20821 ttttgttcga tagttcctgt ataagggcct gtcctgcatg ttctttcaac tcacataatg
    20881 cccatattac actgtcgcgt atctgctcat caggatgtgc aagcaatgta ataagcggaa
    20941 ctatggcatc tttcccgtat tctattattt cttcgaatgc tttattgcga atactatcgt
    21001 cacctgtctg tatgttttcc attatctgct ctatagtatg tggggtttta gcttttgtca
    21061 tatagtaata ctgccttatg tttaagggat tgaattatag taatcaacac ctttgttgaa
    21121 atatatagtg taaattggat attttcaaaa taaaaaatat aattgatttg aatatatgcc
    21181 atgaataatt aaaaagcaag cctattaatt ttacgataca atacatatgc taatagtaag
    21241 cgtgatatgc tatcaaagat gtgaaatatt ctttacaaaa tgtagtaata atgaaagtat
    21301 taaattacca aacaccttac gttctatttt agagaggtca ctatggataa tacccaaaaa
    21361 gtaattgatg catttaaaaa atcaaatgaa cctttaaacg ccagcacaat tgctaaaatg
    21421 acagggcttg ataaaaaaga agttgataaa ataatggaaa agcttaaaaa ggatagcgta
    21481 attgaatcgc ctaaacgttg ttactggact ttgaaataga ttaaatcata acattaatat
    21541 acactatact attgatgaaa caattatttg aggatcatgg ctaccaccaa aaaaagaaat
    21601 tctgctaaaa ctaaaaaatc agcagcaaag aaaaatattt cttcaaagaa acgttcgtct
    21661 acatcttcgc atgctcccta tgtactggta ataatggcat tggcagctgg tttgattttt
    21721 gttctgtcat taaaaactga taccattaaa cagataattc caaaaaccaa tactgttgtg
    21781 gatgcaaata aacataatag tagcaccaat gatgtaagca agaactcaaa gaaggatgat
    21841 aatcataaag actttgacag caaagaaaaa cttaaacagg aactatcgca caaagaaaaa
    21901 aagaaagagg atgaagttgc tactgttcag gcaaaggttt attttttatt ctataatgag
    21961 aaggctgata aaattgaatt gcgtcctgtt gtgcgtgaag tgaatgccag ggtccccgta
    22021 aaagctgtac ttgaagaact cataaaaggt ccaacaaaag tagaagagaa gaaaggctat
    22081 gtgacagcca ttccaccaac tcttaaagta attgatgttt cgatagtcaa tactattgca
    22141 ttcattaatt tcaatggagc gcttgaggaa ggtgcagcgg gaaacattat gcttaatcgt
    22201 cttgatcaga ttgtgtatac cgcaacacaa tttgacaatg ttgatggaat acatatttta
    22261 attaacgggc agcgcaaacg tttccttggt cctgatggta tatcgattgc agggccacta
    22321 aagcgtcatt gaagtatgcg aggtataatg ttagaagttg agattaaatc acgatgtagt
    22381 gatctggatg gtgtaaaaaa aacaatagtg gaatcagggg gaacatttat atccactgag
    22441 gtagaagaag attggtattt taatcatcca ggtcgtgatt ttggaaaaac cgatgaggca
    22501 ttgcggatca gaaaggtcaa tgatatggtt ttttgcactt acaaaggccc aaaacttggt
    22561 ggtaaaacca aaacacgttt tgaaaaggaa gtacagattc atgatgccgt agccatgcag
    22621 gagatattac aaaggttagg atttatattg tatggaacag ttcagaaaaa acgcgatgtc
    22681 tatttgctaa atgaggttac catatgctgc gatgatgtta aaggtttggg aacttttgtt
    22741 gaactggaga tgataagcaa taataaggag gaagctgaaa aaaggctctt tgcgctggcc
    22801 gaaaaattag gactttctga ttttattaca acttcatatt tagaaatggt caaaggagag
    22861 taagctattt tatataaatg ggttaatgat tttaattttg tttttaataa cctggttatt
    22921 ttgtaggtct tcggtgtaaa gaattatgca gttattctta attgcagaag aaattattaa
    22981 actatcccaa tatgaaaatt tatatttcat tgctatatct attgcataaa cgatatctgc
    23041 catatcaata gtagcaatgc taaagttttt aactaagctt gtaattaatt tttttatatc
    23101 agtaaaatgt attttaaatt ttttattaag tacccatata aattcattaa taacttgagt
    23161 gctaagtatt atttcgttaa attctaagag tgatatgact gtatctcttt ttaaaggttc
    23221 agtttctgaa aaggcataaa tcagaatgtt tgtatcgata aatactttct taggcataaa
    23281 tttcttctct gttgaatttc catttttttg tatcaatttt tattttgttt aaaaatatct
    23341 cttttttaac gggatgttta agtcttatga ttacttcaat ttcttgttct ttgaattcat
    23401 caggtatttc tattgttaga tatttgtctt ttacatgata atcttttttt attgctatca
    23461 taatgctacc tctttcaata ctgatttcta aacaacaatg tatcagaata attaaaaaag
    23521 atttgaatta atatatataa aataatataa aagtgtaatt tgtcaattag atattttgaa
    23581 tatttatatt aaggaggatg ggctatgtac aaaaaaatga tgataccctg tattgctatt
    23641 attatgcttt ttactggtta tgcaattttt gccaatgaaa tgttgaaaca aaagattatt
    23701 gcttttgagc aatccatacc atggtcagat gtatatgatg gtttcaaaac acgtcagcat
    23761 tcatggcatc aggatgtcat gatggcaggt tcaccacagg aactggggaa gctccttgtt
    23821 gagttgcaga gtttcattat aaaagaaaag ttgggaagtg attggaataa tatcaaaggg
    23881 gcttggatag ctaaaacagg tagtgccact acctatgatg acattgcagc aagtttaaag
    23941 cagttgcagg attattatct tagcagtgca agacaggatg gcagtgatgt taaaaaagaa
    24001 attattacat ttgaacaata tgttccctgg gaagatgtta aagatgggtt taaaacacgt
    24061 cagcaggggt ggcattatga tgttattaat gcaaaatcac ctcagcaggc gggtttattg
    24121 ttacttgaat ttcatgactg gatatacccc gacaagtttt ctgatgcatg gacagttata
    24181 aaacctgcat gggttaaaaa agcaaagaat gctgcaagct ggcaggatgt aaaaaaccta
    24241 ttaaaagaat tacagtggaa ttacaggaaa gggaaataac cttttttatt gatattgatt
    24301 aattgtataa tcccacgctg taaacgacgg cgtgggatta tttgtaatat aactcatata
    24361 cttttttact ttaaggtata tgattcgaaa tcaggatacc cttcaatccc atgttcagag
    24421 atatcaagcc caataagttc ttcttccctt gtaactctta tgcccatggt tgcgttaata
    24481 ataatccaga taacccagct taacacaaag acaaaactgc caactgcaac cacgccaaca
    24541 agttgattgg caaaagagcc tattccacca ccgtaaaaaa ggccaagagg tggttgccca
    24601 cttccgcctg catacggtgc tgcagcaaaa agacccaccg ctaacgttcc ccaaatgcca
    24661 ttgacaaggt ggactgaaag agctccaaca ggatcatcaa taccccatcg gtcaaaggca
    24721 agaacaaata ccactacaag aatgcctgca atcccgccaa tgacaataga tgcaccggga
    24781 gttacccatg cacagggtgc tgtaattgca actaatccgg ctaatgcacc attaagtatc
    24841 attgataaat caggcttttc cagtgcaagc catgctgtta tagttgcagt taatgtacca
    24901 gcagcggctg ccaggttggt agttacagca attcgcccta tatccgcagc atttgcagcc
    24961 attgttgagc ccggattgaa cccaaaccag ccaaaccaca gaacaaacac acctaacgtt
    25021 gcaagtggaa ggctatgccc gggtatagaa ttgacactgc catcagggtt gtatttacca
    25081 atacgaggac ccagtaccag tgcacctgtc aatgcagccc agccaccaat ggaatgtacc
    25141 actgttgaac cggcaaaatc ccagaagcca cgctttgcca accagccacc accccatatc
    25201 cagtggcctg caatggggta aatgatactg gtaaggatga atgaaaatac aataaatgca
    25261 atgaatttta ttcttccgcc aactgctccg gaaactatcg ttgctgcagt tgcacagaat
    25321 accagttcaa agaaaaattt ggcatatagt ggtacccctg tccagttaag ggaagagtat
    25381 acacctttat acatttctcc ggttacaggg ctgttatcag caccatttaa cataaacatt
    25441 cctttccatc ccataaaacc gttgccatcg ccaaacatga gtccccaacc aataaaccag
    25501 aacgcaattg aagagatagc aaacacaaca aagttctttg ccgatatatt aactgcgttt
    25561 tttgcctgtt gcaagcctgc ctcaaccatt gcaaacccta agttcatgaa gaaaactaga
    25621 aaggctgcaa aaaggaccca cagagtatct gcaacaacag ccattttcac tgaatctgcc
    25681 tgaacctggg taactatagt gttggtttcc tgtgccaata gtaagcctgc acacagcaac
    25741 actacaccaa ttattcctga tatttttaat aacctcatag attcctccta aatttcatag
    25801 taaagtgcat attcccatgg atggggtctt aaagctatct ggtctatctc atgcaatcgc
    25861 ttgtgggcaa tatagctttc aatgagatca acagtgaata cttctccttt taaaaggaag
    25921 gaacaatcac gctcaagacc atcaataact tctttcagtg ttccaggggt attacgtatg
    25981 ttcctctttt catggggcga tagttcatag atatcctcat caatggggtc aggtggttca
    26041 atcctgtttt caatgccatc cattccagcc ataataatgg ctgcaaatgc taaatatggg
    26101 ttagctgaag gatcagggca cctgaactca atacgttttg ccttttcgct ggtgctgtac
    26161 attggtattc taaccgctgc cgaacggttg cgtgccgaat ataccaggtt gatgggtgcc
    26221 tcataaccag gaacaagccg cctgtatgaa ttggtggtag gggctgcaaa tgccagcact
    26281 gcctcaatgt gggcaaaaat accaccaatg aaataccgtg catagtcact taaatctgca
    26341 tacctgccaa agtcatagaa gatattcctg ttttccttcc acaagctgac atgcacatgc
    26401 atacctgaac cattatccat aaaaatgggc tttggcatga atgtaacggt tttattatat
    26461 cggtgtgcaa cattcttgat aatatacttg tacatcacca gtgcatcagc ctgtctaacc
    26521 agttcgtcaa caacaagccc aatctcagtc tggcctgctg ttgcaacttc atgatgatga
    26581 agattgactt taataccgca tgaacgcagt acttccacca tttttgaacg caggttatga
    26641 agctggtcat gtggtgatgt agggaagtat ccctgcttat agggtatctt ataacctaaa
    26701 ttttgttcgc ctctggcaga gttccagcta gcttcagaag agtcaacctc atagaatgct
    26761 ttgttctgag tttgttcgta ctgtacttta tcaaagatga agaattccgg ttcgggacca
    26821 aagtatgcca tatcggctat accggttgag cggatatatt caattgcttt ttcgcctaca
    26881 taccgcgaat ctttggtata ccgtcttctg gctacaggat caatgatatc acaaaggaat
    26941 gaaatggtcc tgtggtcaaa gaatgggtca ataaacgcag tattagggtc agggcgcatg
    27001 agcatgtcgc tttcatgaat agactgaaat ccccgtattg aggaaccatc aaaacctgtt
    27061 cccactgcaa acaactcttc atccgctctg tcagcagtta atgtataatg gtgaagcctg
    27121 ccaaacaggt cagtaaactt aagatccaga aattctatgt tacgttcttt tattatctta
    27181 agtatatctg atatggaggt atgctcctta tgcccattaa ccagatagat agggtcaaaa
    27241 atagcttcca taaatacctc cttattcaag ctcctttttt accggcaaag acttcttttc
    27301 atctaaaatc tttgttaagt ccatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27421 nnnngtgtat ttttttgcat taacatcctt cttcaattct tccagttcat taagatgttt
    27481 cagttcggcc agcttctttg catattctgc tcgcacttct ttctgaaagg ttgattcatt
    27541 caacaatgcc ttgcgttccc tgaaattgtt ttcatattct tcaatggcaa ttctggactg
    27601 aaattcaaca tcacttgctt tttttctggc attgtactca acgatctttg cttccgcttc
    27661 ggcttcagct tctttgatac gggcatcacg cttaacaata gcattgcgtt gacgcccaac
    27721 agcatctaaa tatccctgag agtctttgat ttcttcaatt ttaactgagt ctaaggtaaa
    27781 tcccagtttc attaattcat ctttggctaa atcatatact ttcttttcaa gttctatcct
    27841 ctggtagttg gcttcctctg gcgtgaatgt tgcaataact ccacgcagaa caccttcgat
    27901 ggtattctta gcaatagttt caacctcgga tgaaggtttg ccaaggagcc tttcaacagc
    27961 attttccagt ccaccacctt cttttgaagc aatcttaaca tgagctatcg ccacaatact
    28021 taatgggatc ataccgttgg aaagcgcccc ttctatctcc agagtgatgg ggataattga
    28081 aagtgacaac cgtgaaacgc gctctagaag aggaacacgc agaccataac ctccacgtat
    28141 tacacgatat cccacaactt ctccgctgtg gagtttgcgt ttacgccccg agaaaatcag
    28201 aatctcgttt ggctcgcaaa ttttaagatt gactttgata aaaataagaa ttgctgcaat
    28261 tgcaatgatg attattgaaa taaccagtgc ccaatcaata tttgctatgt acatttccat
    28321 tgtttgcctc cttttaagta attaacctat ggctcttgat ccgcgttcgc ctgtgcggat
    28381 acgaacacat tcctcaattg gtatgacaaa aattttgcca tcgccaatat taccagtctt
    28441 tgctccacga gttattgctt ttatagttgg ctcaacgtaa tcgtcattga cagcaatctg
    28501 aagctcaatt tttttcaaaa ggcgtatctc atgttttaca ccacggtagg tttcggggta
    28561 tcctttctgc ctgcctgagc caattgagtt gattaccgtc attaaattaa tactggcttc
    28621 atttaactcc ttttttacat ccgctaattt ttcgggacgg atgattgcga taattaattt
    28681 cataaaacct ccttactatt tcattaaatt atgtagttgt ggattgtgtt atcattctca
    28741 cccaactaca ttccagtact ggttctatgt atagagtagc agtgaggtat tataggggtc
    28801 aatgtttggt taaaatgagg cgattattaa ccttttattt aacatatgtt taatcatttt
    28861 gtaacataat tgaaacaatt ggtagatata taaattttca tttttttctt gacaattatg
    28921 tcacattgtg tctataagtg gtaaaaagtg gggattagtg cagaatagta gaaatgtaag
    28981 gggaaagtat agtatactat gtttatgggt gaataccgcc ctacgcttga cgaaaaatgc
    29041 cgagttgcaa tacccattaa actgcgtaaa gcgtttggcg aagacaacat catacacaaa
    29101 ttaatcataa ctcatggttt tgataaatgt gttatggctt tcagggaagt tgactggaag
    29161 gaatttgtgg aaacaaagct tgttcccctt tcacaggccg accccatgaa tagaatgcgt
    29221 atcaggtttt tgctgggtgg tgcaacagaa tgtgagctgg ataaacaggg aaggattctt
    29281 atccctgatt atctgcttga gtatgcacag atcagtaagg atgttgtcat gttgggtttg
    29341 tataaccgca tcgaaatatg gagtgcagaa atttatcagc aatacaaacc tgatggagaa
    29401 gcgttgaatc agtttgcaca tgatctggga ttttaacaat gaactatagt gtaatgtgac
    29461 ataatatgga agaaagtaca caatactatc acacccccgt aatgcctcag gaagtgcttg
    29521 cttttctttc tggcattgag ggaatagaat catctattgt ggtagattgt acagttggtg
    29581 aaggtgggca ttcggaatta atccttaaaa catgggatgg attgaagctg tatggatttg
    29641 aacgggacag ggaaattttg gcgatagctc ataaaaggct gtcaccatat gggaatagag
    29701 tggtgttaat taatgacaat tttaaaaatc tctggctcca ttgcaagggg atggaaaata
    29761 gagtagctgc gtttttatat gactttggaa tttcttcgtt ccattttgat ggcagcagta
    29821 gaggatttgc atttaaaaca caggaaaagt tagatatgcg ccttgacgat tcagtgacgc
    29881 ttactgcata tgatgtggtg aataatttca gtgaaaatga attgacacaa atttttagca
    29941 catatggtca ggaacgctgg tcgcgtcgta ttgcccgtaa gattgtccag aagcgaagtg
    30001 aagaagctat tgaaaccaca gagcagttgg caaacattgt gctgcaggca attccaaaac
    30061 agtacagggt taaacatatt catcctgcta cacgggtttt tcaggcttta cggattttta
    30121 ttaatgatga actgtcggca attaatgaat cactgaaaca tgcactggga tatgtaaaac
    30181 caggcggaag aattattgcc atatcatttc attcacttga ggatcgcatt gtaaaggaaa
    30241 gattcagacg atgggccaaa gggtgttgct gcgataatga cgggttgttg tgtcagtgca
    30301 cagctaatcc tcttgttact gtgttgacaa aaaagccact gcagcctaaa gagtccgaaa
    30361 ccagacagaa tccacgttcg cgcagtgcaa aattacgggt atgccagaag gtatgatgag
    30421 catgaataca tcagaacaaa aaagtatagt gcaatacagt accattgttg tcattcttat
    30481 atgtttcatc attgcattca tttatcagaa tattgaatat atgcagctta agatgcacta
    30541 taatgctatg ataaaagcgt atcatacaat acttgaagaa tatgaacgtg aaaagtatca
    30601 gctgaataaa gcactgtcaa tggagcagat agagatattt gcaaaagctc atggtctgga
    30661 gcccatgact aaagaaagcc tggtaatact gaaaagcaaa caaggtacac attaatggat
    30721 atacgaacat ataactcgtt tgacttatat acattactta atgatacaga tgtcacaatt
    30781 ataaaaggtg atgaggatat acgtatcagc gccattgaat atgactcgcg gaaggttgaa
    30841 aaagagggac tgtttgttgc tattgaggga atggaaaccg acggccataa attcataaaa
    30901 gatgctgttg aaaacaaagc atcatgcata gtattgcagg aaaatcgcct ggatgaattt
    30961 ggctttttac ttgatcaaca ggatattgca gttgcaacag caaaggatac aagaagggca
    31021 ctgtcacaga tttcagccag gttctatggt gaaccttcgt tgcaggctgt tgtcattggt
    31081 gttaccggta ccaatggcaa aactaccacc acatatatgc ttgaatcaat attgtcaaga
    31141 gcgggattta cctgtggggt tttaggtact attaactatc gctggaagga taaagtaatc
    31201 aatgcttcac atacaacacc agagtcaaag gacctgcagg agataatgtc aataatggtt
    31261 ctggatggcg ttgattgtat aataatggaa gtttcttccc atgcactatc gctgttacgg
    31321 gttgacgata tacattttga ttgtgccata tttaccaatc tcacgcgcga tcatttagac
    31381 tatcacatta ccttcaatga ctattttatg gcaaaagtgc gtttgtttga aattttaaat
    31441 aaaagcctta aacagaaaaa agcagcattc attaatgctg atgataatta tggcaaacag
    31501 atattaaact ggcgcgagcg ctatggttat ccacttttct cctttggtat caccaacaat
    31561 actgattatc attgcattga atcttcaatt caaatttcaa ttaaaggcac gcaatttgcc
    31621 acggatttac catttgccaa acaatttacc atgaaactgg caggccgatt caatgtgtac
    31681 aatgcattgg gagcaattgg tgttgctgat tttttaaatg tgcctgttgg cagaatagta
    31741 acagggatat cagtgctatc cagtgttccc ggcagatttg acacaattct gtcgcctgaa
    31801 ggttttgcag tggttgttga ttatgcacat accaatgatg cactggaaaa gttattgctt
    31861 tcagtacgtt cattaaaacc tgagcgcatt ataacggtat ttggctgtgg tggtaacagg
    31921 gataaaacca aacgaccatt aatgggcaaa gttagcgagg attattctga ctgggttatt
    31981 gtaaccagtg ataatcccag aaaagaaaat cctgaagcta taatacggga tattacaaca
    32041 ggaatgaaaa caaaccgtta tcaggtaatt gttaatcgtg aagaagcaat caagcatgca
    32101 atatatatgg caaatcagga tgatgttata gtcatagcgg gcaaaggcca tgaggattat
    32161 cagattattg gcacaaagaa gtaccacttt gatgacaggg aagttgcacg caaatatatc
    32221 aatgagaggc ttgggaagtg atagtacggt ttgaaacaac ctcacaaacc attgctactg
    32281 ccattaatgc tattcatcac ggcagtggtc agcctgtgca gtatattact acagattcgc
    32341 ggcttataga agataaaagc ctttttgtgc caataactgg aaacacattt gatgggcatg
    32401 attttataga agatgtatgt aaaactggaa aatgcacctc atttttaacg cataaacagg
    32461 ataaagtaaa tatagcagat cgttataata caagcgccat ttattgcaat gacacattgc
    32521 aggcgtatgc agcaatagca cggtggcaca ggaaaaaatt taacatacca gttattggtg
    32581 ttaccggtac caatggcaag acaaccacaa aagagatgat agcacaggtt ctttcaacag
    32641 aaggccagtg cctgaaaacc cagaaaaatt ataataatga aattggcgtg ccatttacca
    32701 tactgcattt gcagaataca cacagctatg ccgttattga gatgggtatg aatcatcctg
    32761 gcgagatatc acgtctttca caaattgcca tgccaaccat agctgtaatc accaacatag
    32821 gggaggggca tcttgaattt ttaggatcag ttgaaaatgt tgcacatgca aaagctgaga
    32881 tactggatgg tatggctgca gggagcatat gcatactgaa taatgacaca aagcacgctg
    32941 aaattgtaaa gaagaaagca cgtgaaaaaa atataaacgt tataaccttt ggacttacag
    33001 gtgatatagc accagagtcg tatacattgt ttcaggataa aacagtagtg gaatatgatg
    33061 gtgttgaaat tactgtgcct gcgtatggct ttcataatgt atatgcggcg atagctacca
    33121 ttgcagttgc cagaattatg gggaaatcag ttataaaagt aaaagaagct ttgtctaatt
    33181 ttaaacctgt agcaatgcgc agtgatatta aaaaaggtcg ctgtactatc attgatgact
    33241 gttataatgc aaacccacta tcaatggagt atgcatttac aagtgcttca atggtattcc
    33301 ctgataaacg aaagatagct gtattatccg atatgaaaga gcttggaact tattcaaggg
    33361 atttgcattt tacagttgga accagattga aggcttcagg tttttcgcat gttttagtat
    33421 gggggaatga tgcacggtat attgcagaag gtgctatagc tggtggatta gataattcac
    33481 tggttaacgt gtttggttcc aaagaggagc ttattgcttt cctactgcag tttgtgaagc
    33541 acgatgatgt agtgcttgtg aaaggttcgc gttcaatgaa gatggaagaa gtagtaaacg
    33601 cattagaaca gtgaggtaac tatgttttat cactttattt tgcctttaca ggaatatcta
    33661 tcgtttttac gattgtttca atatattacg tttcgttcgg cgtatgctgc attgacatcg
    33721 cttttaatag tattgatttt tggcgggttg gttatacggt ggctgacgca tcttcgcttt
    33781 aaagaagaaa tcaggacatt gggaccacaa tcgcaccaga aaaaagcagg aacgcccaca
    33841 atgggtggcg tactcatatt gggtgcagtg cttatctccg cgcttttatg gggcaatttt
    33901 aacaacctgt atttcatttt acttattgtt attactgttc ttttaggtgc aattggattc
    33961 attgatgatt acattaaatc aatattaaag aaaaaggatg gcattccagc acggcttaaa
    34021 tttttgttgc agtgtgtgtc cgggcttttg attgcactgt gtatttactt tttcccatca
    34081 aatgcaaaag aagcaacaac tttatatgta ccttttatca atagagcaat acttgatttg
    34141 tcctgggtat ggatactatt tgccatgctt gttattgttg gtagttccaa tgctgtaaac
    34201 cttactgatg gacttgatgg gcttgcaatt gggacagtga ctattgttgt tagtgtgctg
    34261 gcagtaatgg catatctcac cggccatcag cctattgcgc agtatttgcg tatcccctat
    34321 atacctcagg cagcggaatt gacagtgttt ttgtcagcac tgggtggtgc tggcttaggg
    34381 tttttgtggt ttaactcaaa tccggcatca gtatttatgg gcgatacggg gtcactggca
    34441 ttgggcggca tcattgggac tgttgccatt atgcttaaaa aggagttttt tcttgtcatc
    34501 gttggtggca tctttgtcat tgaggcacta tcggtcatag tgcaggttgc ttcatataaa
    34561 ttacgaaaaa aaagggtttt taagatggca ccaatccatc accactttga actttcgggt
    34621 tggccagaac agaaggtggt ggttcgcatg tggattattg gtattatact ggcaatactg
    34681 ggtttaagtt cgctgaagat tttataatgg gcaggcgata gttaccatga actatcgccc
    34741 atatatggta gtctttatat tgtaaaggat agttactgtg gagcagaaga aacacatact
    34801 tgtggttgga ctgggttacc ggacaggttt agcctctgcc aactttcttg ttggtaaagg
    34861 ttttgatgtg atagtatgtg atagaaaatc acatgaggaa cttttgcctg ttattcaaaa
    34921 acttgaccag agtgtgaagg tgatagctgg caaccaggat ccatcaattt tagatactgg
    34981 ttttaacgaa attatattaa gtccaggtgt cccacagagt ataccattaa tacaggaagc
    35041 aaagggtaaa ggtataccgg tgatatcaga aattgagctt gcttaccggt attgcacatc
    35101 gcgtattgtg ggtattacgg ggacagatgg caagtcaacg acaaccatgc tgactgcaca
    35161 ttgcctgaaa gcttctggct tcgatgcaca tccatgcggc aatattggca ttccctatgt
    35221 gtcgatagtt gatacattga aaaaagattc agttgcagtg attgaacttt cctcattcca
    35281 actggaaact attgatacat ttaaaccaga tgttgcaatc cttaccaacc ttacgcctga
    35341 ccatttagac cggtataatt cgcttgaaga gtattttgct gcaaagatga atattgcaaa
    35401 aaatcaggat agcaatgatt attttattgt aaacagtgat gatgaattta tagcaacaca
    35461 tctgccttca ttgaaagcaa caaagtttac tttttcactg caaaaagatg ctgactgtta
    35521 ttttaaagaa ggtggaatat attgtacact gaatggcagg caacaattaa tagcacaagc
    35581 tagcaggctt aaaatactgg ggctgcacaa tgtgcagaat gcaatggcgg caatattggc
    35641 tacactatcc cttgcaaaaa aaataggccg tgacgttaat cttaagacac ttgaagatgc
    35701 gttgtactct tttgaaggct tgcctcaccg ggtgcagatg ataggcagct ttgaaggaag
    35761 agcgtttata aatgattcaa aggcaacaac ggttggtgct gttatcatgg cattaaaaag
    35821 tattcccggt gatgtgatac tcattatagg tggcagggca aaaggtgatg attattcacg
    35881 actgaaaaat tatttaaaag gcaaagttaa ggcactggtt ctaataggtg aaacgaagga
    35941 tgagtttgcg caacttttta acgatattcc ccatgtaaaa gcacattcca tggaagatgc
    36001 tgttgttaag gcaatgaagg taagtggaga aggcgatact atacttttat cacctgcttg
    36061 tgcttcgttt gatatgtata aaaattatga agaacggggg aatgatttta ttgaaatctt
    36121 taaaagactt aaacaggggc aattgcggtg gatgtaaagg cagtcattga tacacgcaga
    36181 aaaggcgaac ctgagatggg gctatttata gtggctttca tactcattgg cattggtatt
    36241 gccatgacct acagtgcaag tgcacttttt gcagaaagaa catttggtga ttccctgtat
    36301 ttcttaaaac ggcaattgtt atggagctgt attggtttta taataatgat gtttgttcag
    36361 cagattgatt accggaacta tcagcaatac acaaaaataa tgcttgtgtt tactcttgta
    36421 acgctggtac tggtgctgat accaggattg ggtatttcag ttaaaggatc ttcacggtgg
    36481 ttgggctatg gattactaag gttccagcct tctgaactgg ttaaattatt tatcgttata
    36541 tattttgcaa aagttttttc ctatgagcat cagcgggatg ttaatgtatt acagctgcta
    36601 ttgccactgc ttatcgttgg tgttttcttt ttgcttataa tgcttcagcc tgattttggg
    36661 acggcaatag atgttgcaat catttcggtg gtgcttttgt ttgtttctgg ttttccacta
    36721 atgtatatgc ttggactggc tattttaagc gtgccaatgt tttatctttt gatttaccag
    36781 gtggaataca ggcgtgagcg tatacttgca tttttagatc catggtcgga ccgctttggg
    36841 aaagggtatc atattataca atcatttata gcatttaagt taggtgggct tttaggtgca
    36901 ggcctgggtt caggtactca gaagataaaa cgccttcctg aaccacatac agattttata
    36961 tttgctgtaa ttgctgagga agccgggcta atagggactt ttagtgtagt gattctttat
    37021 ttgctgtttt tttactttgg ggtaaagatt gccctgcgtg caccggatga atatggaaga
    37081 ctgcttgcaa tgggtttaac attacttgtt accgtgcagg cgtttttgaa tattagtgtg
    37141 gttattggtg tgttgccaac aacaggaatc cctttaccat ttgtaagtta tggtgggtcg
    37201 tcactggtgg taaccatgat tgggacaggt attctgttaa atatttcaaa atatcgtgag
    37261 gtagttgccc aggagcttaa atacagcggc gaggtgtggg tatgaaaagg acagtgttga
    37321 tcagtggtgg aggtactggc gggcacataa tgccaggaat agcattgtat gaggaattca
    37381 aatcacgggg gtataatccc atattactgg tgggagttaa tgatgtaaaa ttttcttcat
    37441 taaatacaat agagaatctg cacacatata atgccccaac gatgtggaag aatattttta
    37501 atcttcccac ttttacaatg cggtttatct cggcattatt gtggacaatg aggtttatta
    37561 caaaaaataa tgttgctgca gtcataggca tgggtggata tgtttcagcc ccttctctgg
    37621 ttggagcatt actgaaaaag attcctgtat atctgtgtga gcaaaatgtt gttcctggaa
    37681 tggtaaccaa tctttttgca aagcgggcaa aggcactgtt cacgacattt gaagccacgg
    37741 cggattattg tgatattgaa attgctcact caattattca tactggcaat cccgtccgca
    37801 agcatgtgtt aaaacaggtg gataagaata tagcattaaa acgctttaac atgatgcatt
    37861 gtaaaaaagt tatactggtg ataggcggaa gtcagggagc cttaaaactc aatgaactta
    37921 tgtatgatat gctcactcaa taccctgaaa attttactga tactggcatc atctggagta
    37981 ccggttcata ttcgtatgag caatttcaga aaaagctgca gaatgtggct cacagggctt
    38041 caatattcct tgcaccatat atacttgatg ttgaaaattc gtatgcggca tgtgatattg
    38101 caataagcag ggccggtgcc ggtgtattaa tggagcttgc agcatgggga ataccttcac
    38161 tattaattcc ctatccctat gccgcaaagg accatcagaa gaaaaatgcc atagagtttg
    38221 tgaaagccgg tgcagccgaa atgattgaaa atagtgaagc gacagctcag aaagtggcac
    38281 cactgctttt tgatttgctt gataatgaaa cattgctgtc caggatgtct gcaaaggcaa
    38341 aatcactggc acgaataaat gctgcacagg atatagtaaa tttcattgtg aaagattgtg
    38401 atttggaaaa ataggagtac atgacgtgtt tagaaagtca aaaaagatgc attttattgg
    38461 gattggtggt atcgggatgt ccggtattgc tgagatcctt attaatttgg gttatgaggt
    38521 ttcaggatca gatctgcgtg aatcagaaca aaccaaacga ttgcaaaaaa tgggtgcaac
    38581 catatttatt gggcattatc cctcaaatat aaaggattac catgttgtcg taacttccag
    38641 tgccatacat aaaaacaatc ctgaacttat tgaagcgaaa aataaaaaaa tacctgttat
    38701 acaccgttca gaaatgcttg ctgaactggt t
//
LOCUS       DAORYL010000019        36225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_940_length_36225_cov_13.427481, whole genome shotgun sequence.
ACCESSION   DAORYL010000019 DAORYL010000000
VERSION     DAORYL010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 36225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36225
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_940_length_36225_cov_13.427481"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..2188)
                     /locus_tag="PLV81_05520"
     CDS             complement(128..2188)
                     /locus_tag="PLV81_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOM09593.1"
                     /translation="MIKKFFVICAILACAGGIVAQNVSIKTNKNLNKTSSINTSRAET
                     LAIDTIAAKPVADGEPVVINGKELFKIYSSLGPLSPRERAEGVVKRLNGLLERKTFNR
                     ENLKIVKSEITTDILYEDSIIMSITPGDAEKEAKSADELASIYAEKIAGILINKPQHI
                     IVNSPDDIYTFAVAHKDVIVKSAVALFSVVVFFIVLYYITKLFNKLYALIEQKKGIIF
                     KGIIIKGYEIVSDSTLVSFVILFLKAIKLSMIIGFLYALISVLFLLFPWAKSSAIMSI
                     LKGILLTILTLVIGFGIFKGLKVLMQTLQDNVTNLKGIIIKPLKIKTVTIITEEQGVT
                     IIKKLIFGMYVLLNLLLLYIIIPIVFSYFEFTKTWADALFGYILNPLKVVITSFINFL
                     PNMFFIIVIFAVNYYVIKFAKLIFDEIRDGNIVIPNFERDWADPTYKIVRSLIIVFTI
                     IVVFPYLPGSQSEAFKGVSIFLGILFSLGSTSVVANIVAGAVLIYMNAFKIGDRVKIG
                     DTIGDVVEKTLLITRIKTIKNVVITIPNSIVMGSHIINYTTSAVQGKGLILHTTITLG
                     YDVPWRKIHETLISAALSTENVLKEPAPFVLQTSLDDFYVSYELNCYTDRPEIMARIY
                     SQLHQNIQDKCNEAGIEILSPHYAAVRDGNASTIPANYLPKTYTAPPFVLKIIEKLSK
                     GSGS"
     gene            complement(2212..3111)
                     /locus_tag="PLV81_05525"
     CDS             complement(2212..3111)
                     /locus_tag="PLV81_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOM09594.1"
                     /translation="MEEIIKKFIFDPAIGKIITIFIGMSIIWVLVKSIQRRVLPKIKD
                     NDNRYRARKFSSFIGFLITLLLITIVYSDKLGGLTVAFGVAGAGVAFALQEIIVSFAG
                     WLAIMFGGFYKTGDRVQLGGIKGDVMDIGVLRTTIMEIGQWVDGDLYNGRIVLVANSF
                     VFKEPVYNYSGEFPFLWDEIKIPIQYGSNYAKAKEIILSIAKKVVTDLPEKTKKQWEH
                     LQQKYRLENAQTEPMVSIVANDNWVEYTLRYIVDYRKRRITKTELFDRILAEIESTGG
                     QIKFASATFQLVEAPEIKVKISS"
     gene            complement(3507..4724)
                     /locus_tag="PLV81_05530"
     CDS             complement(3507..4724)
                     /locus_tag="PLV81_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOM09595.1"
                     /translation="MIVGISLLISALCSLLEACLLSMSHTDIAEISENSPKMDTIWNN
                     FKDNIQKPIAVILIINTISHTTGASIAGAQFDELFGSKWIIAFSFIFSFAMIQWSEIL
                     PKTLGVRYNKEIAIIGTYPLVILIKIFTPFIKLIELLNKPFVKKIPASNDGDIVKEIA
                     VLSHYAFINKLISKDQEQIITRTITLSQKKVKDIMIPRDEMKVLYDNMTLMNALVEAH
                     IHNHTRYPLIRHSNQDEVMGYVNFKDIVSALQINPDNPSLKGIARPILIINDEDSFLV
                     LFKKLTRSRQHICIVKDMHDNIVGMVTLEDVIEEIIGELEDEYDELPLYFYPITKTRY
                     LVGGGVKIHEINERIGSSLDDEDLNVDGWMKKHFGFNLKGGDKYNYTNYNFIIKKMVR
                     SKVSELILEKSNE"
     gene            complement(4940..7195)
                     /locus_tag="PLV81_05535"
     CDS             complement(4940..7195)
                     /locus_tag="PLV81_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOM09596.1"
                     /translation="MKKFGTFKGVFVPSFEAILGTVLFLLLPALTVDVGLLPMLMVIV
                     LSHTVTIATAFSIADCATNLNQVGGGGMYALSKRSLGLSFGGSIGIQLFIAQAASIGF
                     YCIGFSEPLYPIISSHLQNVIPAIASGTLFYKQVFSTVILLLFFVIVMFGADFTLKIQ
                     MIILFVLSLSVLSIFAAPFINSGIIEKGIFTGNINLYGMRPITVSLFILTFTQFFPAV
                     TGIDAGVGMSGELKNPKKSLVTGTFASISITFVIYIISAAVYSTIRGDGFIVDYHNNQ
                     PVGRLLTEVLGFNNDFPRNAVGLIVFAGILFATGSSALSCFMTAPRTAQSLARDNVLP
                     RFLHFLSRDFIANGTEPRFAVVVTFFIALAVIWMGNINTAAMIVGICFLTVYGWVNGS
                     AFLERISRNPTFRPTSKGHWLISLYGYAASLIAIFIFSWQLGFLIIAVQLIIFRLILT
                     YKEGGKLEGVWWGVIFTFMTSAINLLNSITQGTKNWRPIVTAISVKGDDRASETVALI
                     AEMLSQHSSFVHYHHIVTSKDLTKIKNGNDGQYDLSIIQSDDPTETVMSMVQMNFYGN
                     LSPNTVLMGFSRKIDNVKIIKKVLSLNKNILLLKNGEKFKDFNTIDIWWRGEKNGNLM
                     VLLAYIINSTMSEKSRKVNIRIIRKLGEREKEKTAQDEMETLLSRARISGEVVIIPHN
                     DEEFLQTVVQVSSKSDLIMMGIPGNYIESQSKSFFNLNEYFFNKEIHKYDTLPTILFV
                     KSASVLNLIED"
     gene            complement(7198..7530)
                     /locus_tag="PLV81_05540"
     CDS             complement(7198..7530)
                     /locus_tag="PLV81_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HOM09597.1"
                     /translation="MKLPEQAVMLKIFIGENDFYKGKPLYEQIVLKARELNLAGATVT
                     KGILGFGADSRIHAAKILTLSEDLPIVIEIVDTQENIQKILPFLDQTVEEGLVTLEKM
                     KVIKYRHR"
     gene            complement(7540..7926)
                     /gene="crcB"
                     /locus_tag="PLV81_05545"
     CDS             complement(7540..7926)
                     /gene="crcB"
                     /locus_tag="PLV81_05545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOM09598.1"
                     /translation="MKHLLVIACGGSLGAVARYIASKNIQSITNQFFPFGTLFVNCSG
                     SFCIGFLFYAFDSLLVSRDIRSFLTIGFLGAFTTFSTYSLETVNLFRDREIKLALVNI
                     LLNNIISISMVIAGMIASKIFLKILQ"
     regulatory      complement(7949..8014)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            8224..9018
                     /locus_tag="PLV81_05550"
     CDS             8224..9018
                     /locus_tag="PLV81_05550"
                     /inference="COORDINATES: protein motif:HMM:NF024515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HOM09599.1"
                     /translation="MKKYLHSLLIIPVLFVCMVTIECSSKSSGNASYSIALSFTRIES
                     PGLDPFRVTITLTKEGSPLPGATLDIAVEKPSSNANISISAVTDKGNGTYEFTVTPDQ
                     TGEYPVTVAYDGVSVTRTPLVMEYVHPEWDQPMSVPGLVNTEGYEDGVTITPDGEYLF
                     VQTGPYRFSSIFVYNEPRVNGGCGGDRLNPSRCEHPWINETCGTYTAPERPGFFNGRF
                     SGTTQLHNANSWGVGIDQAPNYAISTMFYGFKRQSDGTFTKPFYMV"
     gene            8984..9796
                     /locus_tag="PLV81_05555"
     CDS             8984..9796
                     /locus_tag="PLV81_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09600.1"
                     /translation="MAPLPSPFTWFNDLNDAIINPYGLSFRPNADGSYTVIFALSDSY
                     TTDYTADVYTYTGFLGNNINLGDYKLNSDLPNPPLRGSYFPSTFVDLTDNSGTQGNPF
                     LYYTPDGVVRSIWTDDEYDNDADTKKISVYVLTAGTFPTATWTKVVLPDSVNRTGHEA
                     IQPTFAGNGLYFTEDVNVVFAAFSGTHDVTGYETNNNWSTPVVILQKDMTIPGLKPGA
                     THIGKILAIGEPTVATYDGKTELYFVYGYIRGIDPITGIADIDMQAGYVRKK"
     gene            9895..10368
                     /locus_tag="PLV81_05560"
     CDS             9895..10368
                     /locus_tag="PLV81_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOM09601.1"
                     /translation="MNISSRSFTNGGTIPAKYAMKAIAGGMNLSPHVAISDVPKEARS
                     LAIAFVDRHPMARNWVHWLVINIPAHTTEIPEGASLKTMPPESIEMINTFGFAGYGGP
                     QPPRGSGVHTYELACYALTETFKMPKKELTEAEFLKLIKPLLIVQCKMTGNFEHK"
     gene            10550..11245
                     /locus_tag="PLV81_05565"
     CDS             10550..11245
                     /locus_tag="PLV81_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOM09602.1"
                     /translation="MAIAVSHYNTVIDVPIEGVCDSSEISQLSGIESLIKNEPKLHSL
                     LISERCIHNRINALAQDIVRDLNHSRHIDILLVLTGSFIFTSDLSRAIFKHGGINASI
                     HCIKTSVYDKTIKASGEHYRAVTLELSPKHIEGRDLLLVEDIADQGFTLSWLKNYLLH
                     ERNAKSLKICSLLYKRLDNPSQEVQNIRRQLDIDYIGFTVPDVWVAGYGIDAAYEYRH
                     LPFIVRVNEEYFV"
     gene            11555..12157
                     /locus_tag="PLV81_05570"
     CDS             11555..12157
                     /locus_tag="PLV81_05570"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HOM09603.1"
                     /translation="MKKVAIITSAILIMIAYAFALTGEEAVVRFQQKMRSISTISGII
                     SWTTSNGFTYTGTFKYKAPDKLYINLTAPGGKTIVTNGKKLWVFDKSSNVCGVQDVGE
                     GYSGGIAAIVSGYNALLASQGPNGYTIKLRSFDKPYPEIVIVTDANFLLKKLILKDKQ
                     GDSTSYTLSNVIVGENIPTGYFDFKPPTSAQSVKNPLNIQ"
     gene            12275..12760
                     /locus_tag="PLV81_05575"
     CDS             12275..12760
                     /locus_tag="PLV81_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09604.1"
                     /translation="MGVVFQGGTNSINYESWIDTQHGKVKSKGAVYGGGIITTIFVHQ
                     FCGEFSLAYMQYSSDSTPDLSVGHPLATVKGKYFYPLSNVYQPGIGLGLYLDLPPASA
                     DYNGGAGANISFCSLVTLSSEIKTLIDIYAQYGSFGMGESSTKIEGGISVGVVYKVGR
                     L"
     gene            12732..>13162
                     /locus_tag="PLV81_05580"
     CDS             12732..>13162
                     /locus_tag="PLV81_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09605.1"
                     /translation="MLCTRWDDYNTYIIKSGIPSIAAACAVCTSILHTYQYILGISCS
                     PFTYLFDFLTILFVLLCVYTYIYSYKNNWAIVTIKIIIALIWITGAASYAIFATISQC
                     ASTMDKHKGIFYAHVLDTEYKRYNTTALLQCTDKSGTFKAY"
     assembly_gap    13163..13262
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13263..14504
                     /locus_tag="PLV81_05585"
     CDS             <13263..14504
                     /locus_tag="PLV81_05585"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOM09606.1"
                     /translation="TALYLGNRDYIHPHVQFSFKEAGVLHVLAASGLHVGIIIFGIMA
                     ISRLLSATKNFSVLLATVGVCAYLFISDQPVSLIRASLMCVAGALCIVCNAQKHSINI
                     LCITACIILCLYPYELFSAGFQLSFLATAGILLTYTRYHMGLKHFHKAAAPLAVTFAA
                     QLFTMPASVYHFQELSVTAFVANLVIIPLISVYMVAGILTPVMVYAGLANLYAYCINN
                     LYSCIIFCIQFFDQMHGTVYITSPTPLVLYLILLLIPLIYKKPANTIMFALLIAIMVY
                     NNQPHFAHIIHHKSDSSCYVAQTENGSAQCIVTLNSYHDYSQLVQKLKSHAVTRLSLL
                     LPDASFKNCTYAVLLGKEFHLETIYIGKYSPSSIAKLAYMAHIDNIPLVFIQHNQKPL
                     QQPVWKKLNDIYTSMQHKLVK"
     gene            14748..16226
                     /locus_tag="PLV81_05590"
     CDS             14748..16226
                     /locus_tag="PLV81_05590"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOM09607.1"
                     /translation="MAILNISQLVDRFEKNEPVSLSFYYPNENISKTLNALLAKILSN
                     QDKIFLLDMMITILREIIVNATKANAKRAYFKKINLNINDPECYQKGIENFKKDIVGQ
                     LDTLEKDLKDNDLKVTITIKKQDDGIRFSITNPTPIHPVELSRIKTRIEKAKQYLDFT
                     QAYEDVYDDTEGAGLGIVLVILLLKNAGVDPSNYKIGTDGKITQVLLNLPNQLRPEKL
                     TTTIKQQILEQIEGIPTFPDHIIQLLELCRRKDVEIKDITFSIMRDPALTTDVLKLAN
                     SAGFVPGKRIDNLSEAVMTIGLKNLESLLMTISARKILDERFQKFEQIWDHCDKTASY
                     ARTIATVYRINTIVEQVSLCGLLHDLGKIVLLSVNLELTNWIADLVSDRKIRTSTVME
                     EISIGISHSGIGQLIAQKWQFPQYLVEAIQWHHSPLSCDSKYKDHVSITYLANLLCGV
                     ESRKYFYYYCEDEICRQFKIETQDAFIKLHKLCMDTYNKIAQ"
     gene            16232..17911
                     /locus_tag="PLV81_05595"
     CDS             16232..17911
                     /locus_tag="PLV81_05595"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOM09608.1"
                     /translation="MENFTITIAKSGHKTLHYTNGQQTIRIHSAYDPVKEAQRIADQC
                     NPKRASIIIVCGLGLGYHVQALKDKFPGLTIIVIEKDKNLIHLVKQEFPEVFTCAHIV
                     NNEEEIATVLESIDIRSFRGTALLVHRPSYSLHPEFYDALIANIHKQISSRISDLLTR
                     FEFEELWVKNILLNAKQMHTALPVKSLFGKFKGIPGIIVSAGPSLINSLDALSQAYEK
                     ALIVCVDTAYKVLERHGISPHIVMTLDAQTHSIKHFLGITQKPLLLADVVSSPKVTRL
                     IEKKLFSTTAKYYTAPDGRIQRESTPLMDWIQNFTGQIGDIQSGGSVATSAFDLLLTA
                     GCSAIVLVGQDLAYTGREIHSRGTHHNDDWLPAINRFKNLDTINQNVIRKRKIKYVPS
                     NSGSTVITDFVLDLYRSWFEDSAKRVPIPVYNTSTGGAVIANTTFTPLQALVQKWKKP
                     PVSPQEIISHELSHKTPINTQSLFRKFSRLHHRLKKLQTAQGEDAANLYAMLDNEDLD
                     TLCSPFMRKTLFYIARHNLEQQKIDMLIKDAAQAAARELLKIFAKLEDSLI"
     gene            17972..18838
                     /locus_tag="PLV81_05600"
     CDS             17972..18838
                     /locus_tag="PLV81_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09609.1"
                     /translation="MKLHYRRYVFIFFLAIALALTLSSLSVPVGNKVIQYYNNVEINW
                     TDQIIVAKATGKPFVSESGTPLDTSTYEILSVNNARSRAIEEAKDRATESLITALQII
                     QVDPIRSLGDCIQSDDYTQMQLGMYPNNIKYSFKPDGAIATQCTAMLPMGRLLKLLPE
                     ALPAKDIPQLPGKLPSTDYSGLIVDTRGQHFSPMLFPSLFAEDGTEIFGKNFIDSRDA
                     YVYGTVAYCFSDDVAMHHKKAGKNPYYCAALRLYNRCPVIANTDLKKILASSVTRNNL
                     KKGNIIIIIDRD"
     gene            18855..19481
                     /locus_tag="PLV81_05605"
     CDS             18855..19481
                     /locus_tag="PLV81_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09610.1"
                     /translation="MNPIASILQTIVRMINRMIDSIDIRTQQSIKEAFFFLVFLLCIG
                     AVFLGYHLGKQSAKPAGKPYAEITNDVFDLDIKIKKEPGSFQSMLEGALMEQRQQLSD
                     NKLTYPTKEDEPMTMPAKPIDIPKDTKEIQKPLSGLDDTKPVEPINPDYTPAVVKPVD
                     SMKRDKEKPVASPLKPQSEVDTEKKETIMPKGTKELKPMDIRDKVFEQ"
     gene            19478..20275
                     /locus_tag="PLV81_05610"
     CDS             19478..20275
                     /locus_tag="PLV81_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM09611.1"
                     /translation="MNFLHVLIVCSLLVSQPANKEAILETILSEPKAAKIPMQYKTDD
                     DSTQKKGAGTPDDVLLKQGIDFYNAEFYERALQALESLQKNYPQSPFKDQASLWKAKI
                     FVQQQKYTNALQELSTIPQQSGEYPAALFMTGRVNQAMGKPLDAIEYYTRVAGLYPDQ
                     DFSDDALILVAQLYISQKKGNAALSALLKVIKQYSDRETVDDAYYWLGRILSTDPNLK
                     DVELARKVYTIFLKRAQDPAYNAFYSSPLKARVADELKRLDATYFPE"
     gene            complement(20282..20746)
                     /locus_tag="PLV81_05615"
     CDS             complement(20282..20746)
                     /locus_tag="PLV81_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOM09612.1"
                     /translation="MNINAEYVNPFLEAASVVFKQLLNVDLRRGKLVLKEVPQPSHDV
                     AIIIGITGAVTGQVVFSMSLEMVKKIAQTLAPGLSEKDIMNEWKDIVGEVANMITGNA
                     MNLFAYSGKRVEMTTPTVIEGESFTITLIKQTTLGINLYSPFGQLEMNVALK"
     gene            complement(20794..21930)
                     /locus_tag="PLV81_05620"
     CDS             complement(20794..21930)
                     /locus_tag="PLV81_05620"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOM09613.1"
                     /translation="MFDSKHPVIKIVGYVIVGFFVLIIIISFGMPDFLSRMGFDQNTI
                     AKVNGETIGYMDFIRYRDTHMFGKTEDPKQQQRMIIERMIQERLLVQLAKKEGIVVTE
                     KEIKNVIRNRFSDNTGTFNETFFKNFLDRFHMGISDYYKYVQQEIYLGKLQNLLLAGV
                     SVSPLELVSDFRIQNTKIKIQYAFVSNQELAKRYAKEITVTDEEVDAEMKKNPKELKD
                     PKTDRQRIKDKLASDRLEKIKQEMAKKIDQLALAGRPFAEAQGILQGVVSYSNEFKPG
                     DLIREKDEKGRILYPLQESKIFQSDVFSLKQGTTSRCINGFDGMYIFTPLVRYIPGTQ
                     IPDKERQTLEQNLFYTKANSLYISLLTRLFEKSKIIRNQKFEAQ"
     gene            complement(21958..22119)
                     /locus_tag="PLV81_05625"
     CDS             complement(21958..22119)
                     /locus_tag="PLV81_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HOM09614.1"
                     /translation="MIDTKLLDILACPACKGDVEYDARNEKILCTQCKKKYPVKDGIP
                     VMLVEEAEE"
     gene            22548..25985
                     /gene="dnaE"
                     /locus_tag="PLV81_05630"
     CDS             22548..25985
                     /gene="dnaE"
                     /locus_tag="PLV81_05630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOM09615.1"
                     /translation="MDFVHLHNHSEYSILDGAIRIQQLIKFAVDNHMPAIALTDHGNM
                     FGMIEFYEEAHKAGIKPIIGQEFYIAPGSRFHKQSTRASNEENAYHLILLAENYEGYK
                     NLIKLSSLGYIEGFYYKPRIDWELLTRYSKGLIASTACIAGEIPSLILEGKVKEAYTR
                     AQEFADLFGKDHFYLELQYHNLKEQKQVNAHLVEMASKLNIGLIATNDAHYVARDDAY
                     YHDVLLCIQTGKLLLDQGRMRFPNDQFYLKTPQEMEKLFEDYPDALYNTMKIAEMIDC
                     QIPLGNPILPNFEVPAGYTKDSYLTELVYEGAKKRFGGTIPDYVKERIDYELSVIQRM
                     QFSGYFLIVWDFIQFAKNNGIPVGPGRGSAAGSMVSYCLGITELDPIKYNLLFERFLN
                     PERNEMPDMDIDFCALRRDEVIEYVKKKYGEDHVSQIITFNKMKSKGAIRDVARALNI
                     PLPQVNALTKLIHHEDLEEALKNSEELQQLYKSNDAGKQLIDIALKVEGLTRSAGKHA
                     AGVVISRDPLTEHVPLYVDSKDGSISSQYEKTSLEKAGLVKMDFLGLANLTIIDTCIK
                     LIKKHRNIDIDINAIPLDDAKTFKLLQKADTMGVFQLESGGMQNILLKLGPTNFDDII
                     AIVALYRPGPLDSGMVEQFIERKRNPQKIEYLHPSLEPILKDTLGVIVYQEQVMLISQ
                     IMGGFTLPEADKLRKAMSKKKQDLIDAMSSKFVKGAIAKGIHEEIACKTYELMATFGR
                     YGFNKSHSAAYALVSYQTAYLKAHYPLEFMTALLTAQVDKIEDIAKYYADCKAKGIQV
                     LPPDINKSQRDFSIEGKSIRYGFIAIKGVGDKAIESILQARENKGMFKDLTDFFTSID
                     LMTVNKGVLESLIKAGAFDSLHPNRAALFTSVDVMLETARQLQQDIATGQGDLFGATD
                     TSFSRIHIEPASVNDWPENQKLQYEKEVLGIYASSHPLSRYAKDIEKLSCTPIKDLEE
                     NVNGTPITLVGILNNVQIKQSQKGNKFAQAVIEDLTGSIQVLFMPQTLSEYEPLIVSQ
                     EPVVITGTVEIENEKPSRMLAQKVLQLTQFKLTQISELHIIINPIGTDTQLLENIKKL
                     LQRYQGNKPVFFHVRQSNGKENIVKANPLYHIKPHQKLLDSLVSIVGKDGFFYTIGC"
     gene            complement(25982..27058)
                     /locus_tag="PLV81_05635"
     CDS             complement(25982..27058)
                     /locus_tag="PLV81_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09616.1"
                     /translation="MSVVLYYNTNKTMKVSNRIIVITQNKDWEAIASAIASQHQLIIE
                     ENEKLHSLEAIIKEIENSKSTAFLRSSLHSFIREHGIPFLIIIDYPVVAETRTDAIVQ
                     KIFTTLLISFMIIARGSGLANIKGNFFVNITRGDAQLFKHSIIHPEKLLAKLKTNDEK
                     VNAIINLYADQKIFHTLFFVKPCTGATKEDMAHELLAYIDAVKKRHVLIEKIVEKQKH
                     TPSRSKDAATVLVKISEDKIVLDHEIMITRDSAYKKYTAGHIYVLGDWTNIHSRKVAG
                     KVITAIKDGFADWKLGSDDPVIIHLEDALVDHTTAATLAQIAFSELRGFSNIRIYCNE
                     KNYKILEAADGFGLIKKLVFIQKS"
     gene            27235..28089
                     /locus_tag="PLV81_05640"
     CDS             27235..28089
                     /locus_tag="PLV81_05640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOM09617.1"
                     /translation="MSYETASCVLCNTYNFKPLFSKPFENGEEFTLVRCRHCGLEFVN
                     PRPYYNDMSRYYKEYFNQRTDRGYNNYFSEAMHREVLRLLTLNLQDLLFYDFEASLPG
                     EKYALDIGCAAGYFVDYLTHRGWHAEGIDIAQDCIEYAHNTLKVNAYCGDFLSFHFNK
                     QYHCITLWATIEHLHYPDKFIEKIHTLLHDEGMLYLSTCRSSAFSARLLYGKKWRYYN
                     FPHHLYFFSYRTLAKLLQLKGFAVTRFVTYGSGIGKPKTLVRKLADSAAKRLYLGDMM
                     LIAARKEK"
     gene            28093..28962
                     /gene="panC"
                     /locus_tag="PLV81_05645"
     CDS             28093..28962
                     /gene="panC"
                     /locus_tag="PLV81_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOM09618.1"
                     /translation="MKIVNTINELRNEIALQRHHNKKIGFVPTMGYLHQGHLSLVHIS
                     KQHSDYQVMSIFVNKMQFNDAKDYTTYPRDYERDFALAKEAGVDLVFLPDDSEMYQNR
                     LVYVDIEMMTDHLCGATRPGHFRGVLTVVAKLFNIVQPDVSVFGQKDIQQAVIIQKMV
                     KDLDFPVTIIVAPIIREPDGLAMSSRNVHLKGDDRTNAVVIHKSLQKAEELIHQGVVQ
                     SHKLIPAMTDIIASGKPKKIDYVSVVDYETLQPIETLKEKSVIAVAAFFGPTRLIDNM
                     IVEKKGDTYTCVY"
     gene            28950..29405
                     /locus_tag="PLV81_05650"
     CDS             28950..29405
                     /locus_tag="PLV81_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09619.1"
                     /translation="MRILIYCAILFIGFTSCQPSKPLTPQEAFNALKDAYTHNNMQNV
                     AALLSTNSKKRISTVIQNLQSMNKAQLSAFAQYYGIRSDSIKTMDIPEYLLLQKKIAA
                     TRGDDVLLTALSQPIAQVTIAQSKATVQLYNGMSLIFVKEGPYWYFEYE"
     gene            complement(29408..30169)
                     /locus_tag="PLV81_05655"
     CDS             complement(29408..30169)
                     /locus_tag="PLV81_05655"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOM09620.1"
                     /translation="MAQTIQSLDRGLKILEILGKSDKPLSLNEITEYFDIDRSSIFRL
                     LGTLIAHNFVHQDPDTKKYMLGFKIMELSGAFGEQTKIETFLRPVLKYVCDKTQQNTH
                     LAMLHGQDVVFIAVEQPRNSLSMHISVGTREPAYATALGKVLLAFMPQPEKEMLLREV
                     TLQKYTSKTITSVAVYKKHLEAIVNTKYAIDNEEYRLGVVCIAAPVFNHRGVVEYSIG
                     ISGSREQVCPKLDEYISVVKDAGAEASRLLGMPSQ"
     gene            30356..30757
                     /locus_tag="PLV81_05660"
     CDS             30356..30757
                     /locus_tag="PLV81_05660"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOM09621.1"
                     /translation="MFIQFDSVNGVKIDKPFERELKVLMSPDNNANVKDFTFILSTLA
                     PNGGCTDFHSHNESGELMIFLSGTGRAWFEDSVYDITPGTALYAPPGKVHKTLNTGNE
                     PLQIACIFIPAISTDYILKMQEESERARGNS"
     gene            30754..32892
                     /locus_tag="PLV81_05665"
     CDS             30754..32892
                     /locus_tag="PLV81_05665"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOM09622.1"
                     /translation="MKSSFNIAEVYNQNIKNNNLVISEVESKSILSHYGIPTTKGKLI
                     TTPDQITEIPSQLSFPLVAKISAKGLLHKTEIGGVITDINNSQQLVEAFNTLMLRIQN
                     NNIQEFTGILIEEQVSGSVEIIAGLTNDTVFGPTIMAGTGGIYTNLLNDVAFHILPAT
                     EDDIYTMLRKLKGYPLIEGYRGKGINLDELISIFMKLCQLALDGREYIHSIDCNPIIA
                     NKDRCIVADASITLLQTKINDPLITEKPNTSYMDTFFNPESVAVVGASSQENKIGHVI
                     VDCLKKSGYKGSIYPINPKQESILGLPAFSSIKDTPQVPEVVIIAVDLSLVPDVLDEM
                     AAIGSHNAIIISGGGKELGGKREELEATIATKAQKHAIRIIGPNCIGVFDSASQFDSF
                     FYHIDRFTRPPQSNISFITQSGTWGVTFMELSHKTGLAKMISYGNRVDVDEGDMIAYL
                     ASDTKTAVIASYIEGLGRGRKFLESVAIAHNNKKPVVVFKTGRTPQAAHASISHTGAY
                     GGSYTLYHDILEDAGILLTDSMHECFAASVTLSMQPAAKGNRVAMVSNGAGPMVNALD
                     LFSKKGLELASLCEESIQSMKEHFSFFYIVNNPVDITGSATSDDYDYVMTQLMNDDGV
                     DIIMPFFVFQNTPLDEAIIEKMAKHNTVKKKPVICCCTEGAYSYAMKERLIAKGIPVF
                     TEIHHWVTAAYAVMQWGKIMQGGSVCKSSS"
     gene            32874..33704
                     /locus_tag="PLV81_05670"
     CDS             32874..33704
                     /locus_tag="PLV81_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOM09623.1"
                     /translation="MQKLIITAALTGGIHGKEANPALPEQPDEIINAAYECYNAGAAI
                     VHIHARDKSGKQTASKEIFTEINEGIRSRCPLIVQNTTGGPGLPIEKRISSLDAKPDS
                     ASLNCGTVVFFYQGKELPFINLRHEIEAFAQAMIERNIKPELEVYNPSMFLEVENLIA
                     KNLLKPPYYINLVMGVNGMGGFAGTPENLLTMIRHLPPGSIFNVSGIGKYQLTMNTMA
                     IIAGGHVRTGLEDNVFYAKGIMAQSNAQLVERIVRLARELGRDIASPQEAREILEIGR
                     "
     gene            33708..34337
                     /locus_tag="PLV81_05675"
     CDS             33708..34337
                     /locus_tag="PLV81_05675"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF006753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HOM09624.1"
                     /translation="MKIAVINETSAADKNKDIIAALEGRGHDIINAGMKQSGATPELT
                     YIHTGFMAALLLNAKRVDMVVGGCGTGQGFLNAVMQYPEVVCGHIITPLDAWLFTQIN
                     GGNCISLMLNQGYGWASEQNLKFIFDALFSVEAGCGYPEHRKESQKQSRQLLKSISCM
                     THKSMAHIIQVLPEEVINPVLTYPGFLDILDVETLEDETLKNALQNRMR"
     gene            34359..36224
                     /locus_tag="PLV81_05680"
     CDS             34359..36224
                     /locus_tag="PLV81_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOM09625.1"
                     /translation="MTAEIEKRAQSIANKGSIEMAIATGAIEQYQTVTVSEAIVLGLL
                     RQRVTKFFGIFGHGSTDIAEVLRVYEEHGLVKMYNVRHETAAAHAATALGLLTGEVAA
                     VVTSIGPGALHAFAGSLASASNGVGVYHIYGDETTHDEGFNMQQIPKDEQGLFLKLCG
                     VMGNAYALYEPWSVTAALKRGAVTTSLPFAQPFFLLAPMNVQPAVLHNFNLLQLPTAY
                     QHTPCECSDASVFRKAIDAVSQAQRITIKIGNGARGCGKKILELAETLDAAIVSGPSS
                     TGIVPYSHPQYMSVGGSKGSLCGNYAMNEADLVIVIGARGVCQWDCSGTAWKKAQTII
                     NFNTNPYHAFHYNKCISIVGDAQLNLRKFIDYLKNSKIKMGDSSLQWLAALQEKKAQW
                     QSFKKLRFDNPALYDDVWKREVLTQPAAIKTVCDFADKHNYIKVFDAGDVQANGFQIV
                     EDENEKQTITETGASYMGFASSSLLVSGILNKPMIAFCGDGSFMMNPQILIDGVQHNA
                     QGIIVIFDNRRMSAITGLQYAQYGNEYKTSDSVEVDYVGMANSVKGVQGLSGGYSRQE
                     LENALQQALSYKGLSVIHVPVYYGQNELGGMGVFGDWNVGNWCTRVQEEYHRLGW"
BASE COUNT        11356 a         7268 c         6803 g        10698 t
ORIGIN      
        1 acgttgtggc gactggtact actatagtct gattatccat gacatggatc ttttaaaaac
       61 catatccagc acatcaacac cagatatcat tgaaacgtgt aaacaaaacg ccgttatgaa
      121 ccttgtatca actgccgctt ccttttgaaa gtttttcaat aattttgagc acaaacggtg
      181 gcgccgtata tgttttgggc aggtagttag caggtatggt tgatgcattt ccatcccgca
      241 ccgcggcata atgcggcgat agtatttcaa ttcctgcttc attgcattta tcctgtatat
      301 tctgatgcag ctgcgaatag atgcgcgcca tgatttccgg cctgtctgtg tagcaattca
      361 gctcatagct tacataaaaa tcatcaaggc tggtttgcag aacaaaaggc gccggctcct
      421 tcaaaacatt ttcagttgaa agcgctgcac taatgagcgt ttcatgtatt ttcctccagg
      481 gaacatcata acccaatgtg attgttgtat gcaaaataag tccttttcct tgaactgcag
      541 aagtagtata atttatgata tgactgccca ttacaattga attaggaatg gtaattacca
      601 cattttttat agttttaatt cttgtgataa gcaatgtttt ttctaccaca tcgccaatcg
      661 tatcaccaat ttttacccta tcaccaattt taaaagcatt catgtagatg agaacagccc
      721 cggcaacaat gttggcaaca accgatgttg agcctaatga aaaaagaata cccaggaaaa
      781 tggaaacgcc tttaaaagct tcggattgtg atcccggcaa ataaggaaat actactatta
      841 tggtaaaaac aattattaaa ctcctgacga ttttgtaggt agggtcagcc caatcacgct
      901 caaaatttgg aatgacaatg tttccgtctc gtatttcatc aaatatcaat tttgcaaact
      961 taattacata gtagttcaca gcaaatataa ctattataaa aaacatattt ggtaaaaaat
     1021 ttataaaaga agtaataaca accttcaaag gattcaggat atatccaaac agcgcatcgg
     1081 cccaggtttt agtaaattca aaatagctaa agactatggg aattattata tacaacagta
     1141 ataaatttaa aaggacgtac attccaaata tcaacttttt aataattgtc acgccctgct
     1201 cttcagttat aattgttacg gttttaattt ttaaaggttt aattattatt cccttgagat
     1261 ttgtaacatt atcctgcagt gtttgcatta aaactttaag acctttaaat atgccaaacc
     1321 caataacaag agtcaaaata gtaagcagga ttcccttgag aatgctcatt atagcagatg
     1381 acttggccca tggaaaaagc aaaaacaata cagatatcaa agcatataaa aaaccaataa
     1441 tcattgataa ttttattgct tttaaaaaca atattacaaa tgaaaccaaa gtagaatcgc
     1501 taactatttc ataacctttt ataatgatac ctttaaaaat aatacctttt ttctgttcaa
     1561 tcagcgcata tagtttgtta aacaattttg taatgtagta gagaacaatg aaaaatacta
     1621 ctactgaaaa caatgcaaca gcagatttta caatcacatc cttatgcgca acagcaaatg
     1681 tataaatgtc atcaggtgaa ttgacaataa tatgttgagg tttatttatt aagatcccgg
     1741 caattttttc tgcatagatg cttgccagtt catctgcact tttggcttcc ttttctgcat
     1801 caccaggagt tattgacatg atgatagaat cttcatacag gatatcggta gtaatttccg
     1861 attttactat cttcaaattt tccctgttaa atgtttttct ttccagaagg ccattcaatc
     1921 gtttgacaac accttcagcg cgttcccggg gtgacagcgg tccaagggaa gaatatattt
     1981 taaaaagttc tttaccgttt attactaccg gctcgccatc tgcaacaggt tttgcagcta
     2041 tagtatctat agccaaagtc tctgcacgag aagtatttat tgaagatgtt ttattaagat
     2101 ttttatttgt tttaatggac acgttttgtg caactattcc gcctgcacat gcaagtatgg
     2161 cgcatatcac gaaaaatttt ttaatcatac catcctccga tagtatataa tttaagaact
     2221 aattttcact ttgatctccg gcgcctctac cagctgaaac gttgcagagg caaattttat
     2281 ctgtccacct gttgattcga tttctgctaa aattctgtca aacagttccg ttttagttat
     2341 tcttctcttc ctgtaatcca ctatatatcg cagcgtgtat tcaacccaat tgtcgtttgc
     2401 aacaatcgaa accatgggtt ctgtctgcgc attttcaagg cgatattttt gttgcagatg
     2461 ctcccactgt ttttttgtct tctctggcaa gtcagtgaca accttttttg ctatagaaag
     2521 tataatttct tttgcctttg catagttgct tccatattgg atggggattt taatttcatc
     2581 ccacaaaaaa gggaactccc ctgaatagtt atacaccggc tctttgaaga caaagctatt
     2641 tgctacaaga actattcgcc cattgtataa atcaccgtca acccactgcc caatttccat
     2701 tatggttgtt ctcagtaccc cgatgtccat cacgtcgcct ttaattccgc ccaattgcac
     2761 ccggtcaccc gttttataaa atcctccaaa catgattgca agccagccag caaaagaaac
     2821 gattatttcc tgaagggcaa aagcaactcc agcacccgca actccaaaag caacagttaa
     2881 tccgccaagt ttatcgctat atacaatggt tatgaggagc agtgtaatga gaaatccaat
     2941 aaagctactg aatttccttg ctctatagcg attatcattg tccttgattt ttggaagtac
     3001 cctccgctgt atggatttta ccagaaccca tatgatgctc atgccaataa agatagtaat
     3061 aatttttcct attgcaggat caaaaataaa tttcttaata atttcttcca tactaagtcc
     3121 cttaaatata agcattattt gtaacacctg aaatataagc tatgtaaaac atacaagcca
     3181 cttattattt tatagagtga caatattaaa agcaatcttt ttttgcattt gtgtcaatta
     3241 tgcgaaaata atatagggga agctgatatc ttttgccaga ttccattgaa gtttttgctg
     3301 ttacattcac tggaacaaat tcaattgcca cgttcattga agttttcctt atatatcaat
     3361 ataaccatca cggtaccatt ttttccatac cagatgataa tagtcttgaa tacggaaaag
     3421 ttttgcctat ttttcaaaaa caatacattt attaagcgat agtacctttt tgattatatt
     3481 cacattatca attttttatg attactttat tcattgcttt tttcaagaat tagttcactt
     3541 actttacttc ttaccatttt ttttattatg aaattataat ttgtataatt gtatttatcc
     3601 ccacctttta gattaaatcc aaagtgtttt ttcatccatc catcaacatt caaatcttca
     3661 tcatccaggg atgatcctat tctttcattt atctcatgaa tttttacacc acctcccact
     3721 aaataccgtg ttttcgtaat agggtaaaaa tataacggta gttcatcata ttcatcttca
     3781 agttcaccaa taatttcttc aataacatct tcaagagtta ccatgcccac tatattatca
     3841 tgcatatctt tcacaatgca aatgtgctga cgactgcgtg tcaatttttt gaataatact
     3901 aagaaactat cttcgtcatt gatgattagt attggtcttg ctatcccttt taaagatggg
     3961 ttgtcaggat ttatttgtag agcacttact atatctttaa aattcacata gcccataact
     4021 tcatcttgat tggagtgccg tattaaagga taccttgtat gattgtgtat atgtgcctca
     4081 accagagcat tcatcaatgt catattgtca tataaaactt tcatctcatc tctgggaatc
     4141 attatgtctt ttactttttt ttgtgataac gtaattgttc gcgtaattat ttgctcttga
     4201 tctttgctaa ttaatttatt tatgaatgca taatgcgata aaacagcaat ttcttttact
     4261 atatcaccat cattggacgc tggtattttc tttacaaatg gtttatttaa taattcaatt
     4321 agttttataa aaggagtaaa tatttttatt aaaattacca gtggatatgt tccaataatt
     4381 gcaatttctt tgttatacct caccccaagg gtttttggta atatttcaga ccattgtatc
     4441 atggcaaatg aaaatataaa cgaaaaagct ataatccatt ttgaaccaaa taattcatca
     4501 aattgagctc cagcaatgga tgcaccggta gtatgtgaaa tcgtattaat aattaatatt
     4561 actgcaatgg gcttttgaat attatcttta aaattattcc atatggtatc catttttgga
     4621 ctattttcag aaatttccgc tatatcagta tgcgacatgc ttaataaaca tgcctcaagc
     4681 aatgaacata atgcagatat tagcaaacta ataccaacta tcattattaa atatatcatt
     4741 tcacaatcct tattttaaaa ttgtttttga cattggccaa tttatggatc gtaattactc
     4801 ataattcctt tagccagaga aatcttaaat cagattttgt ataatttgtt acctgttgac
     4861 aaactgcaag tatctttatt atacattaaa aaattgcacg tatcaatggt tatagggtta
     4921 taatataatt catttcaaat caatcctcaa tgaggtttag taccgaggcg ctttttacaa
     4981 ataaaatagt gggaagagta tcatatttat gtatttcttt gttaaaaaaa tattcattca
     5041 aattaaaaaa acttttactt tggctctcaa tataatttcc aggtattccc atcatgataa
     5101 gatcgctttt acttgatact tgaactactg tttgcaaaaa ctcctcatca ttgtgtggaa
     5161 taataacaac ctcaccgcta atgcgtgccc tggaaagcag tgtttccatt tcatcttgtg
     5221 ctgttttttc tttttctctt tcacctaact ttcgtataat tcttatgttc acttttctgg
     5281 atttctcact cattgtagag ttaataatat atgccaataa aaccatcaag ttgccatttt
     5341 tttcaccgcg ccaccatata tcaatagtat taaaatcttt aaatttttcg ccatttttaa
     5401 ggagtaatat gtttttgttc agtgacagca cctttttgat aatctttaca ttatcaattt
     5461 ttcttgaaaa acccatcagt acggtatttg gagaaagatt cccataaaaa ttcatctgta
     5521 ccatactcat tactgtttca gtaggatcat cggattgtat aatgctgaga tcgtattgcc
     5581 catcattgcc attttttatt ttggttaaat ccttgcttgt tactatatga tgataatgta
     5641 caaagcttga atgctgagaa agcatctcag ctatgagcgc aaccgtttca gaagctctat
     5701 catcaccctt gactgatatt gcagtaacta tcggcctcca attttttgtc ccctgcgtaa
     5761 tactgtttaa aagattaatt gcactggtca taaaggtaaa gattacgccc caccatactc
     5821 cctcaagctt gccgccttcc ttgtatgtaa ggattagtct gaatattata agttgaacgg
     5881 cgataatcaa aaaacccagc tgccagctga aaataaatat ggcaataaga gatgccgcgt
     5941 agccgtatag tgaaataagc cagtgcccct ttgacgttgg cctgaaggtg ggattgcggc
     6001 tgatgcgctc caggaaagct gaaccgttta cccagccata tacggtcaaa aaacatatac
     6061 ccacaatcat agcggctgtg ttgatgttgc ccatccatat aaccgccagc gcaatgaaaa
     6121 aagttacaac cactgcaaac cggggttcag tgccatttgc tataaaatca cgtgaaagaa
     6181 aatgcaaaaa ccgcggcaat acattatcgc gcgcaagcga ctgtgccgta cggggcgccg
     6241 tcataaagca gctcaatgcg gatgaaccgg tggcaaataa aatgcctgca aatactatca
     6301 agcccactgc gttgcgcgga aaatcgttat tgaaacctaa aacctccgta agcaggcggc
     6361 ccactggctg attgttgtga taatcaacta taaatccatc tccccgtatg gttgaatata
     6421 ctgccgctga gattatgtat attacaaacg taatagaaat tgatgcaaac gtgcctgtaa
     6481 ccaggctttt tttggggttt ttaagctccc cgctcatgcc aacgcctgca tcgattcccg
     6541 tcactgccgg gaaaaactgc gtaaacgtga gtataaaaag cgatacggta attggtcgca
     6601 taccgtacag attgatgttg ccggtgaaaa tgcctttttc aattatccct gaatttataa
     6661 atggtgctgc aaaaatggac aaaacactta acgataaaac aaaaagtatg atcatttgaa
     6721 ttttcagcgt aaaatcagcg ccaaacatca cgataacaaa aaacaagagc agtatgactg
     6781 tagaaaaaac ctgcttataa aagagggtgc ctgaagcgat tgctggaatt acattttgca
     6841 aatgcgatga aataatggga tacagcggct cggaaaatcc aatgcaataa aatccaattg
     6901 atgcggcctg cgcaatgaac agctgaatgc caattgatcc gccaaacgac agccccagcg
     6961 agcgttttga aagcgcatac attcctccgc cgccaacctg gtttaaattt gtagcgcaat
     7021 ccgcaattga aaatgctgtc gcgatggtaa ccgtatggga aaggactatc accataagca
     7081 tgggcaaaag ccccacatcg acggtcaggg cgggaagcaa aaggaaaaga actgttccca
     7141 gtattgcttc aaatgaggga acaaaaaccc ctttaaatgt gccaaatttt ttcatagcta
     7201 ccggtgccga tattttatta ctttcatttt ttcaagtgtc accagacctt cttcaacggt
     7261 ttggtcaaga aatggcaaaa ttttctgaat attttcctgg gtatcaacaa tctctatgac
     7321 aatgggtaaa tcttcagata atgtcaatat ttttgccgca tgaatgcggc tgtcagctcc
     7381 aaaacccaga atccctttgg ttaccgtagc gccagccagg tttaactctc gcgctttcaa
     7441 tacaatttgt tcatacaacg gctttccttt gtaaaaatcg ttttccccaa taaatatttt
     7501 cagcataacc gcctgttctg gtaatttcat gataaacacc tattgtagta ttttcaggaa
     7561 tatttttgaa gctatcatcc ccgctattac catgctaata gaaatgatat tattcaataa
     7621 aatattaaca agcgcaagtt ttatttctct gtctctaaat aaattaaccg tttccagtga
     7681 ataggtagaa aacgtcgtga acgctcccag aaatccaata gtcaaaaaag aacgtatatc
     7741 acgagatacc agaagactat caaatgcata aaacaaaaag ccaatgcaaa atgaccctga
     7801 acaattaaca aaaagagtac caaaggggaa gaactgattg gttatacttt gtatattttt
     7861 tgatgcaata taccgggcta cggctcccag acttccgcca caggcaatga caagtaaatg
     7921 tttcatcgtt ttgactccgc atatactgtt tttaacagga gtcatcagcc atgtgcagtg
     7981 gcggttaacg gtgaacccca tcacctgcgg ataaaattgc taccatagta atttttatca
     8041 atactttttt ttaagtattg agagtagcag aggtatacat tttactttat tggtatcagt
     8101 tgataccact acaaggggca ttaaacaggg aacaaaataa catagcattt gccaaaaaaa
     8161 ctatatcact ttttttatta agacacagta ttattatcat atctcatttt ttacggtgcg
     8221 atagtgaaaa aatatttaca ctccctttta atcattccgg ttttgtttgt gtgcatggtt
     8281 actatcgaat gctcatcaaa gagctccggc aatgcttcct attccatagc attatctttt
     8341 acgcgtattg agtcacccgg gcttgatcct ttcagagtaa ccataacgct caccaaagag
     8401 ggaagcccct tgcccggagc cacactggat atagcggttg aaaagccatc aagtaatgcc
     8461 aacatatcaa tatcagccgt cacggataag ggcaacggca cctatgaatt taccgtcacg
     8521 ccggatcaaa ccggcgagta tccggtaacg gttgcctatg acggcgtgtc agttacccga
     8581 acgccgctgg tgatggagta tgtgcatcca gagtgggatc agcccatgtc ggtaccggga
     8641 cttgtcaata ccgaaggtta tgaagacggt gtaacaataa ctcccgatgg agaatacctc
     8701 tttgtgcaga ctggcccgta tcgctttagc agtatcttcg tgtacaatga gcctcgcgta
     8761 aacggtggat gcggtggcga tagactcaat ccatcacgct gtgaacatcc atggattaac
     8821 gaaacctgcg gcacatacac tgctccagag cgccccggtt tttttaatgg ccgcttttca
     8881 ggcaccaccc agctgcataa tgcaaattcc tggggtgttg gcattgacca ggctcctaat
     8941 tatgccataa gcaccatgtt ttatggattt aaacgccaat ctgatggcac ctttaccaag
     9001 cccttttaca tggtttaatg acttaaatga tgcaataata aacccctacg ggttgagctt
     9061 caggcccaat gccgatggaa gctatactgt aatttttgcg ttgagtgatt cttacacaac
     9121 agactataca gctgatgtgt atacctatac tggtttttta gggaataata taaatttagg
     9181 cgattataaa ttaaattccg atttacccaa tccgccattg cggggcagct atttcccatc
     9241 cacctttgtt gacctgaccg ataatagcgg cacacagggc aatccctttt tatattatac
     9301 acctgatggg gtggtgcgct ccatctggac cgatgatgaa tatgataacg atgccgatac
     9361 taaaaaaata tctgtgtatg ttttgacagc aggaacattc ccaactgcaa catggacaaa
     9421 ggttgtgctt ccggacagcg ttaaccgcac cggccatgaa gcaattcagc caacgtttgc
     9481 tggcaatgga ctgtatttta ctgaggatgt caatgtggta tttgctgcat tttccggaac
     9541 tcatgatgtt accggctatg aaacaaacaa caactggtca acacccgttg ttattttaca
     9601 aaaggatatg actatacccg gactaaagcc aggtgcaacg catattggga aaatccttgc
     9661 aattggcgag ccaacggttg caacttatga tggcaaaacc gaactgtact ttgtgtatgg
     9721 atatattcgc ggcattgacc ccattaccgg cattgcggat attgacatgc aggccggata
     9781 tgtgcggaaa aaataaaaat taataccctc aattgctata aaatatactc ttgacttttc
     9841 attatttctc atatacatat ttgcaaatat atagtataga aaaaggagga agccatgaat
     9901 atctcatccc gctcgtttac caatggtgga actattcctg caaaatatgc tatgaaagcc
     9961 atagcaggtg gtatgaatct atcaccacat gttgccatta gcgatgtacc caaagaagcc
    10021 cgttcccttg caatagcctt tgttgatcgg catcccatgg cacgtaactg ggtgcactgg
    10081 cttgttatca acattcctgc acataccact gaaatccctg aaggtgcgtc acttaaaaca
    10141 atgccccctg aatctattga aatgataaac acatttggct ttgccggata cggtggtcct
    10201 caacctccac gtggcagcgg cgtgcataca tacgaacttg cctgctacgc cttaacagaa
    10261 acgtttaaaa tgccaaaaaa agagcttacc gaggctgaat ttttaaagct tataaagccc
    10321 ttgctcattg tccagtgtaa aatgacggga aattttgaac ataaataggc tgaatagtat
    10381 acaataaaac agttgactat tattgtgttt tccaccaatt ttaacaaatt caatatttag
    10441 aattgctatg tatttctgaa aaatgtaaat gtattatctc attgtgaagt atggtatatt
    10501 tcactgccga aagcggcaga catataaatt attttaaaaa ggatgcacca tggcaattgc
    10561 tgtttctcat tataatactg ttattgatgt acccatagaa ggtgtgtgcg atagttccga
    10621 aatcagccag ctatccggga tagagtcact cattaaaaac gagccaaagc tacattcgct
    10681 gcttataagc gaacgttgca tacacaacag aatcaatgca cttgcacagg atattgtacg
    10741 cgacctgaac cactcacggc atattgacat attgctggta ttaacaggtt catttatctt
    10801 cacatcagac ctgtcgcgcg caattttcaa acatggtgga atcaatgcca gtatccattg
    10861 tataaaaacc agcgtgtacg ataaaaccat aaaagccagt ggtgaacatt accgggcagt
    10921 aacactggaa ctatcgccca aacatattga agggcgcgat ctgctgctgg ttgaagatat
    10981 agccgatcag ggatttaccc tgtcatggtt aaaaaattac ctgctgcatg aacgcaatgc
    11041 taaaagttta aaaatatgca gcctgctgta caaacgcctt gacaatccat cacaggaagt
    11101 tcagaatatt cgcaggcagc ttgacattga ttatattggt tttaccgttc ctgacgtatg
    11161 ggttgcagga tacggtatag atgctgcata cgaataccgg catctgccat tcattgtacg
    11221 cgtgaacgag gaatatttcg tgtaacgggg ctgtcctaaa agaggtcgtt gagtaaattt
    11281 tgcaaacatt atatcgtaat actcgctcat ttcgatacaa ttttgccaca aaatctggca
    11341 aaattactca atgagcgttg cgaaatttgt atcgaaacga ctttggggac actccttttg
    11401 gatgcgggaa aggacctgaa cggacgctat tgtgcagccc gcaatggaat gggtaataat
    11461 gattaaaaat tatttatgta aaaaattttt ttattttttc acattcacgc gtattatata
    11521 ataaaagata ataaaatcat tgttggtgat aaaaatgaaa aaagtagcaa taataacttc
    11581 agcaatattg ataatgatag cgtatgcttt tgcccttacc ggtgaagaag ctgtcgtgcg
    11641 ttttcagcaa aagatgcgaa gcatctcaac aataagcggc atcatatcat ggaccacatc
    11701 aaacgggttt acctatacag gaacatttaa atacaaagcg cctgacaagc tgtacataaa
    11761 ccttactgcc cctggcggca aaacaatagt aaccaatggc aaaaagctgt gggtatttga
    11821 taaatcatca aatgtatgtg gcgtgcagga tgttggtgaa gggtattctg gtggtatagc
    11881 agctattgtt tcaggatata atgctctgct tgcttcacag gggccaaacg gatacaccat
    11941 aaaactcaga agctttgata agccatatcc tgaaatagtc attgttaccg atgcaaattt
    12001 tttactgaaa aaattaatat tgaaagataa gcagggtgat tcaaccagct atacattatc
    12061 aaacgtaata gttggagaaa atataccaac aggatacttt gattttaagc caccaacaag
    12121 cgcacaatct gttaaaaatc ctttaaacat ccagtgacag ggtaaatgag tgaaagtata
    12181 taaaaataaa aaaatatttc tctgtgtaac cactattacc tgtatttttt tttcagcacc
    12241 aattgtacgt gcgcaggaat tgttcttcac atatatggga gttgtttttc agggtggcac
    12301 caattccatt aattatgaaa gctggattga tacccagcat gggaaagtaa aaagcaaagg
    12361 tgccgtttat ggcggtggca ttattacaac tatttttgta caccagtttt gcggcgagtt
    12421 ttcactggcc tacatgcagt attcatccga cagtacacct gacctgtcag tggggcatcc
    12481 actggctacg gtcaagggaa aatatttcta cccactttcc aatgtttacc agcctggtat
    12541 tggtcttgga ttatatttag atttgccacc agcttcagct gattataacg gtggtgccgg
    12601 tgcaaatatc tctttttgtt cactggtcac tctttcatca gaaataaaaa cattaataga
    12661 catatatgcc cagtatggca gctttggcat gggcgagtca tcaacaaaaa tagaaggcgg
    12721 aatttcggta ggtgttgtgt acaaggtggg acgattataa cacatatatc atcaaaagtg
    12781 gcataccctc aattgcagca gcctgtgcag tgtgtacgtc gattttgcac acataccagt
    12841 atatactggg aatctcatgt agtcccttca catatttgtt tgatttcctt acaattcttt
    12901 ttgtattgct atgcgtatat acatacatct attcatacaa aaataattgg gcgatagtaa
    12961 ctatcaaaat catcattgcc cttatctgga ttactggtgc agcctcttat gcaatctttg
    13021 ccactatttc acagtgtgcc agcacaatgg ataaacacaa aggcatattt tacgcacatg
    13081 tgcttgatac tgagtacaaa cgctataaca ccacagcgct tttacagtgc acagataaga
    13141 gtggtacatt caaggcttac agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13261 nnactgcatt gtaccttggc aacagggact acattcatcc tcatgtgcag tttagtttta
    13321 aggaagcagg tgttttgcac gtacttgcag caagcggact tcatgttgga atcatcatat
    13381 ttggcatcat ggcaattagc cggttactat cggccacaaa gaatttttca gtactccttg
    13441 ccactgtggg tgtatgtgct taccttttta tttcagatca gccggtttcg cttatacggg
    13501 catcacttat gtgtgtagcg ggcgccctgt gtattgtatg taatgcgcaa aaacacagca
    13561 tcaacatcct gtgcataact gcctgtatta tcctgtgtct gtatccttat gaattattta
    13621 gtgctggttt tcaattatcg tttttagcaa cagcaggaat acttttgaca tatacaaggt
    13681 accacatggg actgaagcat ttccacaaag ctgcagcgcc acttgcagtt acctttgcag
    13741 cccagctttt taccatgcca gcaagtgtat accattttca ggaactatcg gtcactgcat
    13801 ttgtggctaa ccttgtcatt ataccactta tcagcgtgta tatggttgcc ggtatactaa
    13861 cgcctgtgat ggtatatgca ggtttagcta atctgtatgc atactgcatc aacaatcttt
    13921 attcatgcat catattctgt attcaattct ttgaccagat gcatggcaca gtgtatatca
    13981 cttcaccaac accgcttgta ctatatctga tattgttgtt aattcccctt atatataaaa
    14041 agcctgcaaa tactatcatg tttgctctgc tgattgctat catggtatac aataatcagc
    14101 cgcacttcgc tcatattatt catcataaaa gcgatagttc ctgttatgta gctcaaactg
    14161 aaaatggcag tgcacagtgt atcgttaccc tgaacagtta ccatgattac agtcaactgg
    14221 ttcaaaaact caaaagtcat gcagtgacca gactttcact gcttttaccc gatgcttcat
    14281 ttaaaaactg tacctacgca gtactgctgg gaaaagagtt ccaccttgaa acaatatata
    14341 tagggaagta ctccccatca tcaattgcaa aactagccta tatggctcac attgacaaca
    14401 ttccactggt ctttatacag cataaccaaa agccgctgca acaacctgta tggaaaaagc
    14461 ttaatgacat atatacttca atgcagcata aattggtaaa ataaagcgat tcttgtagca
    14521 ataataattc ctaaaaaaaa gtattttaca tgtatattat acttctggcg cattcggcag
    14581 atcaataaat agatcattta ttaatttgaa aaaagttaga aaaaaataat gactgttact
    14641 ctaatgataa ttccaaaaat aaaatgattg tatttaactt tgctctatgg tatattcttc
    14701 cgataatgat atatatttga agaattatcc atattataag gaatactatg gcaatattaa
    14761 atatatctca gcttgtagat cggtttgaaa aaaatgaacc ggtaagcctt tcattttatt
    14821 atcccaatga aaatatatca aaaaccctga atgcactgct tgcaaaaatc ctttccaacc
    14881 aggataaaat attcctgctt gatatgatga ttactatttt gcgggaaata atcgttaatg
    14941 ccaccaaagc aaatgctaaa agggcttatt tcaagaaaat caatttaaac attaatgacc
    15001 ccgaatgtta tcaaaaaggt atagagaact tcaaaaaaga tattgttggg caattagaca
    15061 cactggaaaa ggatctgaag gataatgact tgaaagttac cataaccata aaaaagcagg
    15121 atgatggaat acgattttca attactaacc ctactcctat acatccggtt gaactatcac
    15181 gcattaaaac gcgtattgaa aaagcaaagc agtatttaga ctttacccag gcctatgaag
    15241 atgtatatga tgatactgaa ggggcaggcc ttggaatagt attagtgata ttgcttttaa
    15301 aaaatgcagg tgttgaccca tcaaattata aaataggaac tgatggcaag ataactcagg
    15361 tgttactcaa tctgcccaac caattacgac cagagaaact tacaacaacc ataaaacagc
    15421 agatacttga acaaattgaa ggcatcccta cgttccccga ccatatcatt cagcttcttg
    15481 aactttgcag aaggaaggat gttgaaatta aagacattac attttccatc atgcgtgacc
    15541 ctgcactcac taccgatgtg ttgaagcttg caaattcggc tggatttgtg cctggcaaac
    15601 gcattgacaa cttaagtgaa gcagtcatga caattggtct aaaaaacctg gaatcattgc
    15661 ttatgacgat aagtgcccga aaaatactgg atgagcgctt ccagaaattt gaacagatat
    15721 gggaccactg cgataaaaca gcttcttatg cacgaactat cgccaccgtg taccgaataa
    15781 acaccatcgt tgagcaggtt tcattgtgtg gcctgctcca tgatttaggc aagatagtac
    15841 ttctttcagt taatctggag cttaccaact ggatagctga ccttgtatca gaccgcaaaa
    15901 ttcgtacgtc aacagtaatg gaagaaattt caataggcat aagccattca ggcattggac
    15961 agcttattgc acagaagtgg caattcccac aatatctggt tgaggcaata cagtggcacc
    16021 attcaccatt gtcatgtgat agcaaatata aagatcatgt ttctattacc tatttagcta
    16081 accttttgtg cggagttgaa tcaagaaagt atttctatta ctattgtgaa gatgaaatat
    16141 gtcgacaatt taaaatagaa acacaagatg ctttcatcaa actgcataaa ctctgcatgg
    16201 atacctataa caaaattgcg cagtaaatct catggaaaac tttacaataa ccatagcaaa
    16261 aagtggccac aaaacactgc actatactaa tggtcagcaa acaatccgca ttcattcagc
    16321 atacgaccct gttaaagaag cgcagcgcat agccgatcag tgtaacccta aacgtgcttc
    16381 aatcattatc gtttgcgggc tgggactggg ataccacgta caggccttaa aagataaatt
    16441 ccccggactg actatcatag ttattgaaaa agacaaaaac cttatacacc ttgttaagca
    16501 ggaattccct gaagtattta catgtgcgca catagtcaat aatgaagaag agatagccac
    16561 agtgcttgaa tcaattgata tcaggtcgtt cagaggaacc gccctgctgg tacaccggcc
    16621 atcgtattcg ttgcaccctg aattttatga tgcactaata gcaaacattc acaagcagat
    16681 aagctctcgc atcagtgacc ttttaacccg ctttgaattt gaggaactat gggttaaaaa
    16741 catattgctc aatgcaaaac agatgcatac cgcactaccg gtaaagtcgc tatttggcaa
    16801 atttaagggc ataccgggta tcattgtatc agccgggcct tcattaatta acagcctgga
    16861 tgcgctgtca caggcgtatg agaaagcttt aattgtttgt gttgacactg catataaagt
    16921 gctggaacgc catggtataa gcccccatat agttatgacg cttgatgcac aaacacacag
    16981 cataaaacat tttctgggca taacacagaa accacttctg ttagcggatg ttgtcagcag
    17041 ccctaaagta acccgtttga ttgaaaaaaa attattcagc acaaccgcaa aatactacac
    17101 tgcccccgat ggcaggatac aacgtgaatc tacaccactt atggactgga ttcagaattt
    17161 caccggtcag attggcgata ttcaatcagg gggttcggtt gccaccagtg cgtttgactt
    17221 actactcacc gccgggtgca gtgcaatagt gctagtgggt caggatttag cttacacagg
    17281 cagggaaatt cacagccggg gcacccatca taatgatgac tggcttcctg ccataaaccg
    17341 ctttaaaaat cttgatacta tcaaccagaa tgtcatccga aaacgcaaaa taaaatatgt
    17401 cccatccaac agtggcagca ctgtcatcac cgattttgtc ctggatttgt accggagctg
    17461 gtttgaggac tcagcaaaac gagtacccat accggtgtac aacaccagta cgggcggagc
    17521 agtgattgcc aatacaacat ttacaccact gcaggctctg gttcaaaagt ggaaaaagcc
    17581 tcctgttagt ccacaggaga ttatatccca tgaactatcg cacaaaactc ccatcaatac
    17641 acaatccctt ttcaggaaat tttcacgtct gcaccacagg ctaaaaaaat tgcaaactgc
    17701 acaaggagaa gatgcagcaa acctgtatgc aatgcttgat aatgaagatt tggacacgct
    17761 gtgttccccc tttatgcgca aaacattgtt ctatattgct cgccataacc ttgaacagca
    17821 gaagattgat atgttaatca aagatgcagc gcaggcagca gcacgggaac ttttgaagat
    17881 atttgcaaaa cttgaagact cattaatttg actatatttc agtgtcattt tgtccgataa
    17941 ctttaaaaac gaaactacta tgttgcatac tatgaaacta cactacaggc ggtatgtttt
    18001 tatttttttc ctggcgatag ctctggcatt gaccctttct tcactatcag tgcctgtggg
    18061 caataaagtt atccagtact acaacaatgt agaaatcaac tggaccgatc agataattgt
    18121 ggcaaaagca acaggaaagc cttttgtttc tgaaagcggc acaccacttg atacctcaac
    18181 ctatgagata ttgtcagtga acaatgcacg cagcagggca atagaagaag ctaaagacag
    18241 ggctacggaa tctcttataa cggcactgca gataatacag gttgacccca tacgttcact
    18301 gggcgattgt attcagagtg atgattatac gcagatgcag ttgggcatgt accccaataa
    18361 tatcaaatac agctttaaac ctgatggcgc tattgctaca caatgcactg ccatgctccc
    18421 catgggaaga cttttaaaac ttttgcctga agcattaccg gctaaggaca taccgcagtt
    18481 gcctggtaaa ctgccatcaa ctgactattc agggctgatt gtggatacac gaggacagca
    18541 cttttcaccc atgctttttc cgtcgctatt tgctgaagat ggtaccgaaa tatttggcaa
    18601 gaattttatt gattcccgtg atgcctatgt gtatggaact gttgcctatt gttttagcga
    18661 tgatgttgcg atgcatcata aaaaagctgg taagaatcca tactactgcg cagcattgcg
    18721 tctttataac aggtgcccgg ttattgccaa tactgactta aaaaaaatcc ttgcaagcag
    18781 tgtaacaaga aataacttga aaaaaggcaa tattattatt attattgata gagactaata
    18841 agaagcacaa tcatatgaac cctattgcat ccatattaca aactattgta cgaatgatca
    18901 acaggatgat tgattctatt gatattcgaa cacagcaatc cataaaagag gctttctttt
    18961 tccttgtctt tcttctatgt attggtgcag tatttctggg atatcatctg ggcaaacagt
    19021 cagcaaagcc agctggcaaa ccgtatgcag aaataacaaa tgacgtattt gacctggata
    19081 tcaaaataaa gaaggaacca ggctcattcc agtccatgct tgaaggtgca ttgatggaac
    19141 agcgccagca attatcagac aataaactaa cctaccccac aaaagaagat gaaccaatga
    19201 caatgcctgc aaaacctatt gatattccta aagataccaa ggaaatacaa aaacctctgt
    19261 cggggcttga tgacactaaa ccggttgaac ccatcaatcc cgattataca ccagcagtgg
    19321 taaagcctgt tgattcaatg aaacgcgaca aggaaaagcc tgttgcttct ccgcttaagc
    19381 cacaatcgga ggttgatact gaaaaaaaag aaaccattat gccaaaaggc accaaagaac
    19441 ttaagcccat ggatatacgt gataaggtgt ttgagcaatg aattttttac acgtactcat
    19501 agtatgctcg ttactggtat cacagccagc aaataaagaa gctatactgg aaaccatttt
    19561 atctgaacca aaagccgcta aaatccccat gcaatataaa actgacgatg attctactca
    19621 aaagaaagga gcaggtactc ctgatgatgt gttgctgaaa cagggcattg acttttataa
    19681 tgctgaattc tatgaacggg ctctacaggc cctggaaagc ctgcaaaaaa attaccctca
    19741 aagtcccttt aaagatcagg caagtttatg gaaagcaaag atttttgtac agcagcagaa
    19801 atatacaaat gccttacagg aactatcgac cataccccaa caatccgggg aatatcctgc
    19861 ggcacttttt atgacaggca gggtaaacca ggcaatgggc aagccactgg acgctatcga
    19921 atactacacc agagtagcag gattataccc tgaccaggat ttttctgacg atgcgctgat
    19981 tctggttgca cagctctata tatcgcaaaa aaaaggcaac gcagcgttaa gtgcattact
    20041 gaaagttatc aagcagtaca gtgaccgtga aacagttgac gatgcatact actggttagg
    20101 caggatttta agtactgacc ctaatttgaa agatgttgaa ctggcacgca aagtatatac
    20161 aatattcctg aagcgtgctc aggatcctgc atataatgcc ttttattcat caccattgaa
    20221 agcaagagtt gctgatgagc tcaagcgttt agatgcgacc tatttccctg aataacacta
    20281 ttcatttcaa tgcaacgttc atttccagct gcccaaatgg gctgtaaaga ttaatcccca
    20341 gagtggtctg ttttattaag gtgatagtaa aactttctcc ttcaatgacg gtaggtgttg
    20401 tcatctcaac gcgctttcct gaatacgcaa aaaggttcat tgcattcccg gtaatcatgt
    20461 tggcaacttc accaacaata tccttccatt cattcattat atctttctca gataatcccg
    20521 gagcaagggt ctgggcaatc ttttttacca tctctaagct catactgaaa accacctgac
    20581 ctgtgaccgc tccggtaata ccaatgataa tagcaacatc atgtgatggc tgtggtactt
    20641 ctttcaatac cagctttcct cttcttaaat caacattaag aagttgtttg aatactacac
    20701 ttgctgcctc taagaacgga ttaacatatt cagcattaat attcatacag taccccttgc
    20761 tgtgtaacaa aaattaaaac caccttgtac cttctactgt gcctcaaact tctgattcct
    20821 tattattttc gacttttcaa acagtcttgt taacagtgat atatataaag aattagcttt
    20881 ggtgtaaaaa aggttctgtt ccagtgtctg gcgctcctta tcaggaatct gcgtgcccgg
    20941 tatatatcgt accaaaggcg tgaaaatata catcccgtca aatccgttaa tacaccgcga
    21001 cgttgttcct tgctttaaac taaaaacatc tgattggaat atctttgact cctgtaaagg
    21061 atacagtatc cgcccctttt catctttttc ccttatgagg tcgcctggct taaattcatt
    21121 ggaatatgac accactccct gcaatatccc ttgggcttct gcaaatggcc tgcctgcaag
    21181 tgcaagctgg tcaatctttt ttgccatttc ctgtttaatt ttctccaacc tgtcactggc
    21241 aagcttatct tttattcgtt gtcggtcagt ttttgggtct ttcaactctt ttgggttctt
    21301 cttcatttct gcatcaactt cttcatcggt aactgtaatt tcttttgcat accgctttgc
    21361 cagttcctga tttgacacaa acgcatactg tatttttatt tttgtgttct gtattctgaa
    21421 atcagacacc agttccagag gtgacacgct gactcctgcc agcagcagat tctgtaattt
    21481 acctaaatat atctcctgtt gcacatattt ataataatcc gatataccca tgtggaatct
    21541 atccagaaaa ttcttaaaaa atgtttcatt aaatgttcca gtattatcag aaaatcggtt
    21601 gcggataacg ttttttattt ctttttcggt taccactatg ccctcttttt ttgcaagctg
    21661 aaccaggagc ctttcctgta tcatgcgttc tataatcata cgctgctgct gtttagggtc
    21721 ttcggtttta ccaaacatgt gcgtatcacg atagcgtata aaatccatat accctattgt
    21781 ttcgccatta acctttgcga tagtattctg atcaaaaccc atacgggata aaaaatcagg
    21841 catgccaaac gatattataa taataagaac aaaaaaacca acgataacat aacccactat
    21901 cttaataaca ggatgtttag aatcaaacat acatataacc tcatatcaaa cattaaatta
    21961 ttcttcggct tcttctacaa gcatgacggg aataccgtct ttaacaggat atttcttttt
    22021 gcactgtgta cacagtatct tttcattacg cgcgtcatat tcaacatccc ctttacatgc
    22081 agggcaggct aaaatgtcaa gtagctttgt atcaatcatc gtaaaaactc ctctcccttg
    22141 aaaaattgat ttactgatac taataagata gattcttccc acgattacgt cggggtcaaa
    22201 atgacagata ataataagat ttctcctcac tgtgttcgtc gaaatgacaa tataataaca
    22261 atgttcgtcc agacgatata gtataataaa ccatagtaaa ttcttttttc aattcttgca
    22321 atttgttttt tcttatatct tttgtatagt ttaccttcat tttgctaaaa tctatcgcac
    22381 atctgtatta cggtcaacaa catattgcaa aaagtataaa aaatattaat acttgcttgc
    22441 aaaatcaaaa tacagggagt atattggtta cataccaaat atttattatg tcgtatttca
    22501 tgagcgatag ctcatggcaa taatcccttc cttaaagcga gaacagtatg gattttgtac
    22561 atcttcataa ccattctgaa tattctatcc tggatggtgc tatacgtatt cagcaactga
    22621 taaagtttgc tgttgataat catatgccgg ccatagcatt aacagaccat ggcaatatgt
    22681 ttggcatgat tgaattttat gaggaagcac ataaagctgg cataaagcca ataattggac
    22741 aggaatttta tattgcgcca ggatcacgct ttcataagca aagcacccgc gcaagtaatg
    22801 aagaaaatgc ctatcacctc attttacttg ctgaaaatta tgaaggatat aaaaatctta
    22861 taaaattatc ctcactgggc tatatagaag gcttttatta caagccacgt attgactggg
    22921 agttgctgac cagatacagt aaagggctta tagcaagcac cgcttgcata gctggcgaaa
    22981 taccatcact tattttagaa ggcaaagtaa aagaggccta cacacgggca caggaatttg
    23041 ctgacctttt tgggaaggac catttttatt tagaacttca gtatcataac cttaaagaac
    23101 aaaaacaggt aaatgcacat cttgttgaaa tggcttctaa actgaatata ggtttaatag
    23161 ccaccaatga tgcacattat gtagcaagag atgatgccta ttaccatgat gtgttactct
    23221 gcatacaaac tggtaaattg ttacttgatc agggcagaat gcgtttcccc aatgaccagt
    23281 tttatctcaa aactcctcag gaaatggaaa aactatttga agactaccca gatgctctgt
    23341 ataacaccat gaagatagcc gaaatgatag attgccagat accactgggc aaccctatcc
    23401 ttcccaattt tgaagtccca gctggataca ctaaggacag ctatcttact gagctggtgt
    23461 atgagggtgc taagaagcga tttggcggca ccatccctga ttacgtcaaa gagcgcattg
    23521 attatgaact atcggttata caacgcatgc aattttctgg ctattttctc attgtgtggg
    23581 attttatcca gtttgcaaaa aataatggaa tacccgttgg cccgggtaga ggttcggctg
    23641 ctggttcaat ggtttcatat tgcctgggga ttactgagct tgatcccatt aaatacaacc
    23701 tgctatttga gcggttttta aaccccgagc gaaacgaaat gcctgatatg gatattgact
    23761 tctgtgctct gcgccgtgat gaagtaatag agtatgtaaa aaagaaatat ggcgaggacc
    23821 acgtaagcca gataataacc tttaataaaa tgaaatccaa aggcgccatt cgtgatgtag
    23881 cacgtgcatt aaacatcccc cttcctcagg tgaatgcact gaccaagctt attcatcatg
    23941 aagatctgga agaagctctt aaaaattctg aagaattgca acaactctat aaatcaaatg
    24001 acgcaggtaa acagcttatt gatatagcgt taaaagttga agggctgact cgttcagccg
    24061 gtaaacacgc cgcgggtgtg gttatatctc gtgatccctt aaccgaacat gtacccctat
    24121 atgtggattc aaaagacgga agtatttcat ctcagtatga aaagaccagc ttagaaaaag
    24181 ccggccttgt caagatggac tttttagggc ttgcaaacct taccatcatt gatacatgca
    24241 tcaagctaat aaagaaacat cgtaatattg atattgatat taatgcaatt cctcttgatg
    24301 atgcaaaaac cttcaagctg ttacaaaagg ctgataccat gggtgtattc cagcttgaat
    24361 cagggggcat gcagaatata ctccttaaat taggcccaac caattttgat gatattattg
    24421 caatcgttgc tctttaccgg ccgggccctc ttgactcggg tatggttgaa cagttcattg
    24481 aaagaaaacg caatcctcag aaaatagaat acctccaccc ctctcttgaa cccatactca
    24541 aagatacatt gggggttatt gtataccagg aacaggttat gcttatatcc cagattatgg
    24601 gtggttttac gctgcctgaa gctgataagc tcaggaaagc aatgagtaaa aagaaacagg
    24661 atttgattga tgcaatgagc tcaaaatttg ttaagggagc aatagcaaag ggtatacatg
    24721 aagagattgc ctgtaaaacg tatgagttaa tggcaacatt tggaagatat ggatttaaca
    24781 aatcccattc tgctgcgtat gcacttgtat catatcaaac ggcttatctt aaagctcact
    24841 atccgttaga atttatgact gccctattaa cagcgcaggt tgataagatt gaggatattg
    24901 caaaatatta tgccgactgc aaagcaaagg gcattcaggt gcttccacct gacatcaata
    24961 aaagccagcg tgatttttcc attgaaggga aatccatacg ctatgggttt attgccatta
    25021 aaggtgttgg tgataaagca atagaatcaa tactacaagc acgtgaaaac aaaggcatgt
    25081 tcaaagacct caccgacttc tttacctcaa ttgatcttat gacagtgaac aagggtgtac
    25141 ttgaatcact cataaaagct ggagcatttg attcgctaca cccaaaccgt gcagcattgt
    25201 ttacttcagt ggatgttatg cttgaaaccg cccgccagtt gcaacaggat attgcgacag
    25261 gtcagggaga tttatttgga gccaccgata catcattttc gcgtatccat attgaacctg
    25321 catcagttaa cgattggcct gagaaccaga agttacaata tgaaaaagag gtattgggaa
    25381 tttatgccag ttcacacccg ttaagccgtt atgctaagga tattgaaaag ttatcgtgca
    25441 caccaataaa agacctggaa gaaaacgtca atggaacacc cataacgctt gttgggattc
    25501 tcaataatgt acagattaag cagagtcaaa aagggaataa atttgcacag gctgtcatag
    25561 aggaccttac cggcagcatt caggttctat tcatgcctca gacattgtct gaatatgaac
    25621 cccttattgt gtcacaggag cctgttgtta ttacaggaac tgtggagata gaaaatgaaa
    25681 agccatcccg tatgctggca caaaaggtat tgcagcttac gcaattcaaa cttacccaga
    25741 taagcgaatt gcacattatt attaacccta taggtaccga tacgcagcta ctggaaaata
    25801 ttaaaaaatt actgcagcgt tatcagggaa acaagccggt atttttccat gtgcgccagt
    25861 ccaatggtaa ggaaaatatt gtaaaagcta acccgcttta tcacatcaag ccacaccaga
    25921 agctactgga tagcttagta agcattgtgg gaaaagatgg atttttttat actattggct
    25981 gctaagattt ttgtataaat actagttttt ttataagccc aaacccatca gccgcttcaa
    26041 gtattttata atttttttca ttgcagtata tccttatatt actgaagccc ctcaattcac
    26101 taaaagcaat ttgtgccagc gttgcagctg tggtatggtc aaccagtgca tcctcaagat
    26161 gaatgataac agggtcatcg ctgccaagct tccagtcggc aaaaccatcc ttgattgcag
    26221 tgataacctt cccggcaact tttctgctat gtatatttgt ccagtctccc agtacataaa
    26281 tatggcctgc agtatatttt ttataggcac tatcgcgggt tatcataatc tcatgatcca
    26341 gcacaatttt atcttcactt atttttacca gtactgttgc tgcatcctta cttcgtgaag
    26401 gagtatgctt ttgcttttct acaatctttt ctattaatac atgacgcttt ttaacggcat
    26461 caatataggc caatagttca tgtgccatat cctctttggt ggcaccagtg catggcttaa
    26521 caaaaaacag agtatgaaat attttttgat cagcatacag gttaataatt gcatttactt
    26581 tttcatcatt tgtttttaac tttgccaaaa gcttttccgg atggattata ctatgtttga
    26641 ataattgtgc gtcccctctg gtgatattga caaaaaaatt acctttaata tttgcaagtc
    26701 cactgccacg ggcaataatc ataaatgata tgagtaatgt ggtaaaaatc ttttgcacaa
    26761 tagcgtctgt gcgtgtttca gcaactactg ggtaatcaat tattatcaaa aatgggatac
    26821 catgttcccg gataaatgaa tgaagtgaag agcgtaaaaa tgctgttgat ttggaatttt
    26881 ctatttcttt tatgattgct tcaagtgaat gaagtttttc attttcttca ataatcaatt
    26941 gatgctggga ggcaattgca gaagctatgg cctcccagtc tttattctgt gtgataacaa
    27001 ttattctatt ggaaactttc atggttttat ttgtattgta atataataca acgctcactc
    27061 agtcatcata ttctaaattt ttgtgacatt gtttgtcgta agaatacatt tgttaataac
    27121 cctatactat taatcatata aagtcaagac tattgtatag cttcaggaat caattactgt
    27181 ttatatgata aaaaatactt gccaaatgca tataatgcct gcgacaatta atacatgagt
    27241 tacgaaactg cctcctgtgt tctatgcaat acatataatt tcaagccatt attttcaaag
    27301 ccatttgaaa atggcgaaga atttacactg gtgcgttgca ggcactgtgg ccttgaattt
    27361 gtcaatccca gaccatatta taatgacatg agcagatact acaaagaata tttcaatcag
    27421 cgaaccgacc gtggctacaa taactatttt tctgaagcaa tgcacaggga agtactgcgt
    27481 ctgctcacac tgaatcttca agatctactg ttttatgatt ttgaggcatc attaccgggt
    27541 gaaaaatatg cgttagatat tggctgcgct gcagggtact ttgttgatta tttaacacac
    27601 cgcggatggc atgcagaagg tatagatatt gcacaggatt gcatagagta tgcacacaat
    27661 actctgaaag taaatgcata ctgtggtgac tttctatcgt ttcattttaa caaacaatac
    27721 cactgcatta ccctgtgggc aactattgag catctgcact accctgataa atttattgaa
    27781 aaaatccata cattgcttca cgatgaggga atgctgtatc tttcaacctg tagaagcagt
    27841 gcattttcag cacggctact gtatggcaaa aaatggcgat actacaattt cccccatcat
    27901 ctttattttt tttcatacag aacacttgca aaattattgc aattaaaagg gtttgccgtt
    27961 acacggtttg ttacatacgg cagtggcatt ggtaaaccaa aaacactggt acgaaaactt
    28021 gctgattccg cagcaaaaag gttatattta ggggacatga tgctcattgc agcacggaag
    28081 gagaaataaa ctatgaaaat agtgaataca attaacgaat tacgtaacga gattgcttta
    28141 caacgacatc acaacaaaaa aataggattt gtacccacca tgggatacct tcaccaggga
    28201 cacttaagcc tggtacacat ttcaaaacaa cattctgatt atcaggtaat gagcatcttt
    28261 gtgaataaaa tgcaatttaa tgatgccaaa gattatacca catacccacg ggattatgag
    28321 cgtgactttg ccctggcaaa ggaagctggt gttgaccttg tatttcttcc tgatgatagt
    28381 gagatgtatc aaaatcgcct ggtgtatgtt gacattgaaa tgatgactga ccatttgtgt
    28441 ggcgcaacac gccccggaca tttcagagga gtgcttaccg ttgtagcaaa gctttttaac
    28501 attgttcagc cggatgtatc agtatttggg cagaaggaca tacagcaggc tgttataata
    28561 cagaaaatgg ttaaggactt agattttcct gttactataa tcgttgcacc aattatccgc
    28621 gaacctgacg gcctggcaat gagttcacgc aatgtgcacc tcaaaggcga tgaccgcact
    28681 aacgctgttg ttatacataa atcgctccaa aaagcagaag aacttataca tcagggtgta
    28741 gtgcaatcac ataaactgat accggcaatg actgacatta ttgcttcagg aaagccaaag
    28801 aagattgatt acgtttcagt agttgattat gaaacacttc agcctattga aacactcaaa
    28861 gaaaaatcgg ttatagctgt agctgcattt tttggaccta cacggttaat agacaacatg
    28921 atcgttgaaa aaaagggaga tacatacaca tgcgtatatt gatatattgt gctattctgt
    28981 ttataggttt tacttcctgc cagccatcaa aaccacttac tccgcaggaa gcatttaatg
    29041 ctctcaaaga tgcttataca cataacaata tgcaaaacgt tgcggcattg ttatccacta
    29101 acagcaaaaa aaggatttct acagttattc aaaatctaca atcgatgaat aaagcacagt
    29161 tatcagcatt tgcacagtac tatggcatac gctctgatag catcaaaact atggatatac
    29221 ctgaatatct cttattgcaa aagaaaattg cagcaacccg tggggatgat gttcttttga
    29281 ctgcactcag tcagcccatt gcacaggtaa ctatcgccca atcaaaagcc actgtgcaac
    29341 tatacaacgg catgagtttg atttttgtca aggaaggacc ttactggtat tttgaatatg
    29401 aataatatta ctgcgaaggc ataccaagca gccgcgatgc ttcagcacca gcatctttaa
    29461 ccactgaaat atattcatct aatttaggac aaacttgctc ccgtgaacct gatatgccaa
    29521 ttgaatattc aaccacacct ctatgattga acactggcgc agcaatacat acaacaccca
    29581 gcctgtattc ttcattatcg attgcgtatt tagtattaac tatagcttca agatgttttt
    29641 tgtaaacagc aactgatgtt atggtctttg atgtatattt ctgcaatgtt acttcacgca
    29701 aaagcatttc cttttctggt tgtggcataa aagcaagaag caccttcccc agtgcagtgg
    29761 catacgcagg ctcacgtgtt cctactgata tatgcatact gaggctattt cgtggctgtt
    29821 caacagcaat aaatacaaca tcctggccat gcagcatagc caggtgcgta ttttgctgag
    29881 ttttatcaca aacatattta agaacaggcc gtaaaaatgt ttctattttt gtctgttcgc
    29941 caaaagcacc ggaaagttcc atgatcttga aacccagcat atacttcttt gtatctgggt
    30001 cctgatgaac aaaattatgt gcaatcagcg tccccaaaag ccggaaaatt gatgaacggt
    30061 caatatcaaa atactctgtt atttcattca gggaaagtgg tttatctgat tttcccagta
    30121 tttccagaat ctttaatcct ctgtccagtg actgtattgt ttgtgccata aatataccca
    30181 atcgtgtttc gtattatgca acaacagtaa cgacaaaaaa gtcaacaatt atattatttc
    30241 aacattattt cattttctgc attactaaga atgaactatt cagtaaaaaa ttaattaaat
    30301 tcttattgac aataatacaa tattattgca ttatatacaa taaaggagat gcaaaatgtt
    30361 tatacaattc gactcggtta acggtgtaaa aatagataaa ccctttgagc gtgagttgaa
    30421 agtactgatg tcaccggaca acaatgctaa tgtaaaagat tttacattta tactttcgac
    30481 acttgctccc aatggaggct gtacggattt tcactcgcat aatgagtccg gggaactaat
    30541 gatattttta tcaggtacag gcagggcatg gtttgaagac tctgtatatg atattacacc
    30601 aggaacagca ttgtatgctc ctcctggcaa agtacacaaa acattgaata caggcaacga
    30661 gccgctacag atagcctgta tttttattcc tgcaatatca actgattata tactgaaaat
    30721 gcaggaagaa tcagaaaggg cacgaggaaa ttcatgaaat catcgtttaa tattgccgaa
    30781 gtttataatc aaaatatcaa gaataataac ctggtaatat ccgaggtaga atcaaaatca
    30841 atcctttcac actatgggat acctaccaca aaaggaaagc ttataacaac gccagatcag
    30901 attacagaaa tcccttcaca attatcattc ccattggtgg caaaaatatc cgcaaaaggg
    30961 ttgttacata aaaccgaaat cggtggagta ataactgata ttaataattc tcagcagctt
    31021 gtagaagcat ttaatacatt aatgttacgg attcaaaata ataatattca ggaatttacc
    31081 ggcatattaa ttgaagaaca agtttctgga tccgttgaaa ttattgcagg gcttaccaat
    31141 gatactgttt ttggtccaac tatcatggct ggcacaggcg gcatttatac aaatcttttg
    31201 aatgatgttg cattccatat tcttccagca acagaagatg atatatatac aatgttgagg
    31261 aaattaaaag gctatccttt aattgaagga tacagaggta aaggcattaa tctggatgaa
    31321 ctcatcagta tatttatgaa actatgccag cttgccctgg atggaagaga atatattcac
    31381 tccattgatt gcaatcccat cattgcaaac aaagacagat gtattgttgc tgatgcttca
    31441 atcacattgt tgcaaacaaa aattaatgac ccgcttataa ctgagaaacc caacaccagc
    31501 tacatggata cattttttaa tcctgaatca gtggcggttg ttggcgcatc gtcacaagaa
    31561 aataaaattg ggcatgttat tgttgactgc cttaaaaaaa gcggctataa aggttcaatt
    31621 tatcccataa atccaaaaca agaatcaatt ttaggtttac ctgcattttc atcaatcaaa
    31681 gatactccac aggttcctga ggttgttatt attgcagtgg atttaagtct tgttcctgat
    31741 gtccttgatg agatggcagc cataggatcc cataatgcaa ttattatttc tggtggtggc
    31801 aaggagcttg gtggaaaacg ggaagaactg gaagcaacaa ttgcaacaaa agctcaaaaa
    31861 catgcaatac gaataatcgg gcctaactgc attggcgtgt ttgatagcgc ctcgcagttt
    31921 gattcatttt tttatcatat tgaccgcttt acacgaccgc cgcagagcaa catcagtttt
    31981 attacacaga gtggaacatg gggtgtaaca tttatggaac tatcgcacaa aaccggtttg
    32041 gctaaaatga taagctatgg caaccgtgtt gatgttgatg agggcgatat gatagcctat
    32101 ttagcttctg atacaaagac tgcagtcatt gcatcttata ttgaaggact tggcagggga
    32161 agaaaatttt tagaaagcgt ggcgatagct cataacaata aaaagccagt tgtggttttt
    32221 aaaaccgggc gcactcctca agctgcacat gcatccattt cccataccgg tgcatacgga
    32281 ggaagttata cattatatca tgatatcctg gaagatgctg gaatactttt aactgatagc
    32341 atgcacgagt gttttgctgc aagtgtaaca ttatccatgc aaccagcagc aaaggggaac
    32401 agggttgcca tggtttccaa tggtgcaggg cccatggtta atgctcttga tctatttagc
    32461 aaaaaaggtc ttgagcttgc atcgctctgc gaagagtcaa ttcaatcaat gaaagaacat
    32521 tttagcttct tttatatcgt aaacaatcct gttgatataa cgggttctgc aacatctgat
    32581 gactatgatt atgttatgac acaattaatg aacgatgatg gagtggatat catcatgcca
    32641 ttttttgtat ttcagaatac accccttgat gaagcaatca ttgaaaagat ggcaaagcat
    32701 aatactgtaa agaaaaaacc tgttatttgc tgttgtacag aaggcgctta ttcatacgcc
    32761 atgaaggaaa gactgattgc aaaaggtatt cctgttttta ctgaaataca tcactgggtt
    32821 actgctgcat atgctgtgat gcaatgggga aaaattatgc agggaggaag tgtatgcaaa
    32881 agctcatcat aactgcagcc cttaccggtg gaatacatgg taaagaagca aaccccgccc
    32941 tgcccgagca gcctgatgaa ataattaacg ctgcatatga gtgctataat gctggtgcag
    33001 caatagttca tattcatgcg cgcgataaaa gtggcaagca gaccgcttca aaagaaattt
    33061 ttacagaaat caatgaaggg atacgaagcc gctgccccct cattgttcaa aatactaccg
    33121 gtggtcctgg tcttcctata gaaaagcgga tctcatcact tgatgctaag cctgattctg
    33181 catccctgaa ttgtggcact gtggtctttt tttatcaagg gaaagaactc ccattcataa
    33241 acttacgcca tgaaatagag gcatttgcgc aagcaatgat agagcgtaat ataaaacctg
    33301 aacttgaagt atacaaccct tcgatgtttc ttgaggttga aaaccttatt gcaaaaaatc
    33361 ttctaaaacc cccatactat attaatctgg ttatgggtgt caatggtatg ggaggttttg
    33421 ccggaacacc ggaaaatcta ttaaccatga tacgacatct gccgccagga agtattttta
    33481 atgtgtcagg tattggcaaa tatcagctta ccatgaatac catggcaatt atagcaggtg
    33541 gtcatgtacg cacagggttg gaggataatg ttttttatgc taaaggtatt atggcacaat
    33601 ccaatgcaca gcttgtagag cgcattgtgc ggctggctcg tgaattggga agagatattg
    33661 catccccaca ggaagcacga gaaatattag aaattgggag atagtatatg aagattgcag
    33721 tcattaatga aacaagtgca gcagataaaa ataaggatat aattgcagcg ctagagggaa
    33781 gaggccatga catcattaat gcaggtatga agcaatcagg tgcaacccct gaacttacct
    33841 atatccatac tggatttatg gcagccttac tgttaaatgc caaacgtgtt gatatggtag
    33901 ttggcggttg tggaacaggg cagggatttt taaacgcagt tatgcaatat ccagaggtgg
    33961 tatgtggtca catcattaca ccgcttgatg cctggctttt tacacagatc aatggcggca
    34021 actgcatttc gcttatgctc aatcagggct atggatgggc ttcggagcaa aatttaaaat
    34081 ttatctttga tgcgctgttc agtgtggaag caggatgcgg atatcctgaa caccgaaaag
    34141 aatcacaaaa gcaatcacgg caattactca aatcaatttc atgcatgaca cacaaatcca
    34201 tggcgcacat tattcaggtt ttgcccgaag aggtcataaa tcctgtgctg acttatcctg
    34261 gttttctgga tatactggat gtagaaactc tggaagatga gacattgaaa aatgcactac
    34321 aaaatagaat gcgataatta ttcttttagg agaaagcaat gactgctgaa atagaaaaac
    34381 gcgcacaatc tattgcaaac aaaggttcta ttgaaatggc aatagctaca ggtgctatcg
    34441 aacaatatca aactgtaaca gtgtctgagg ctattgtact tgggctttta cgacagcgtg
    34501 tgacaaaatt ttttggaatc tttggccatg gctcaaccga cattgctgaa gtattacggg
    34561 tatatgaaga gcacggcctt gttaagatgt ataatgtacg ccacgaaact gccgcagcac
    34621 acgcagcaac agcacttgga cttttaacag gtgaagttgc tgcggttgtg acatcaatag
    34681 gccccggtgc actgcatgcg tttgctggct cactggcatc tgcttcaaat ggtgttggtg
    34741 tgtatcacat ctatggcgat gaaacaacac atgatgaagg ctttaacatg caacaaattc
    34801 caaaagatga gcagggattg tttttaaaat tgtgcggtgt gatgggaaat gcctacgccc
    34861 tgtatgaacc atggtcggtg acggctgcat taaaacgcgg tgctgttacg acaagtctgc
    34921 catttgctca accgtttttt ctgcttgcac ccatgaatgt gcagccagct gtgttacata
    34981 actttaattt attgcagcta cccacagcat atcagcatac gccctgtgag tgcagtgatg
    35041 cgtctgtatt cagaaaagct attgatgccg ttagccaggc tcagcgcatc accataaaga
    35101 ttggtaacgg ggcacgggga tgcggcaaaa agattcttga gcttgctgaa actttagatg
    35161 cagccattgt gagcggtcca tcttctactg gcattgtacc atactctcat ccacaataca
    35221 tgagcgttgg cggttccaag ggttcactgt gtggaaacta tgccatgaat gaagctgatc
    35281 tggtgatagt tatcggtgca cgtggggtgt gtcagtggga ttgctcagga acagcgtgga
    35341 agaaggcaca aactattata aatttcaata caaatcctta ccatgcattc cactataata
    35401 aatgtatttc tattgttggt gatgcacagc taaatctcag gaaatttatt gattatttaa
    35461 agaactcaaa aattaaaatg ggcgatagtt ctttgcagtg gcttgctgcc ttacaagaaa
    35521 aaaaagcaca gtggcagtcg ttcaaaaagc ttcgttttga taaccctgca ctgtacgatg
    35581 atgtgtggaa gcgtgaagtc ctaacccagc ctgctgcaat taaaaccgta tgcgattttg
    35641 ctgacaaaca taattacatc aaggtgtttg atgcaggcga tgttcaggcc aatggatttc
    35701 agatagttga agatgaaaat gaaaaacaaa ctatcaccga aactggtgct tcatacatgg
    35761 gatttgcgtc atcttcattg cttgtatcag ggatactcaa caaaccaatg attgcattct
    35821 gtggcgatgg cagcttcatg atgaatccgc aaatccttat tgacggagta cagcataacg
    35881 cacagggcat tattgtcatc ttcgacaacc gacggatgtc cgctatcaca ggtttgcagt
    35941 atgcacagta tggcaatgaa tacaaaacat ctgattcagt ggaagtggat tatgtgggca
    36001 tggcaaattc agttaaagga gtacagggat tatccggtgg atattcccgt caagaattag
    36061 agaatgcatt acagcaggca ctatcttaca agggactgtc ggtcatccat gtaccagtat
    36121 actatgggca aaatgaactt ggtggcatgg gtgtatttgg ggactggaac gtgggcaact
    36181 ggtgcacccg cgtccaggag gaatatcaca ggctgggatg gtaag
//
LOCUS       DAORYL010000020        35674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_967_length_35674_cov_14.421011, whole genome shotgun sequence.
ACCESSION   DAORYL010000020 DAORYL010000000
VERSION     DAORYL010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 35674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35674
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_967_length_35674_cov_14.421011"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="PLV81_05685"
     CDS             <1..888
                     /locus_tag="PLV81_05685"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOM09626.1"
                     /translation="CADTKKETITVFHAGSLSALFKECKVTFEKKHNYTLLLEASGSV
                     DAARKLTDLHKQCDVIALADVELFDSMLKSYCPYWIAFAGNEMVLAYTEKSKFAQAIA
                     TNWMDAFLRYDIICTRSDPLRDPCGYRALLVWKLASQYYKNTELEKIFAMRSPLAYMR
                     PKEIDCIALLETGACDAMWIYKSVAVQQNFPYITLDEHINLGSHTYEHIYRKACITLS
                     DTKKQYTFCGSTITYGASIPVTANNVKGAIAFLSFLCSKEGQGLINKHGFTSIVPYSN
                     NSEQLPVELKKVVAGGNRK"
     gene            872..1675
                     /locus_tag="PLV81_05690"
     CDS             872..1675
                     /locus_tag="PLV81_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOM09627.1"
                     /translation="MVTVNKKNIVFYSIVVVLSFVVAFLVVVPIASMVLSVSVRDVVD
                     ILFDREVVQSLLLTFECAFYATLIAVLFGIPLAYLFAFTHFPLKRLIITLFDIPMIIP
                     HTASGIALLFVFGSGFLSHLFKSLHMSFVGSKTGIVIAMAFVSIPYFINAVQNGYASI
                     DRRLINVARTLGASQTQCFVHIILPLSVRAIITGSLMMWGRGISEFGAVMIIAYHPMI
                     APVLLYDRYSAYGLEGSRPIAALLIVFCIVFFILIRSLLAYREKHAYTT"
     gene            1659..>1921
                     /locus_tag="PLV81_05695"
     CDS             1659..>1921
                     /locus_tag="PLV81_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOM09628.1"
                     /translation="MLTLHNIQWESKQFTLTIPSLQFHDGAHAMIMGSSGSGKTLLLS
                     IIAGIIAPHHGTIYSDNEDITHIPPETRGFSIVYQEPALFAHMT"
     assembly_gap    1922..2021
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2022..2570
                     /locus_tag="PLV81_05700"
     CDS             <2022..2570
                     /locus_tag="PLV81_05700"
                     /inference="COORDINATES: protein motif:HMM:NF015424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOM09629.1"
                     /translation="ELIGILNHIKKEYNPTVLHVTHDFDETLQLGNYLYIMSKGAIIQ
                     HGTVTDVYRYPSDIFVAQMVGVKNIFHGSVVHKKDGAYFVTDNGIAFFLGDSYRKFFQ
                     YAILRAEDIIISRKLLHSSATNQFKGTVIDVTEVKGLYKVSVDIGEIVQSMITKRSLQ
                     AMNIKKGAKVVVIFKGSAIHLF"
     gene            2792..3325
                     /locus_tag="PLV81_05705"
     CDS             2792..3325
                     /locus_tag="PLV81_05705"
                     /inference="COORDINATES: protein motif:HMM:NF016611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM09630.1"
                     /translation="MKRLFNSFMVTTILCFISSVTIAQHIEEAVQLNTKGLFYLECGN
                     YEKAYEYFMLALQHDPTNKLLYNNCAVACMNMKKYQEAYRMLSIALAIDPYYVKALSN
                     MAIVNFHLLKFADAYTYYSKALMVDSSYTKERFRLDKVIAGVKKVQSENPENDDLKEI
                     VRQLEILKNNNQDIYSK"
     gene            complement(3317..3766)
                     /gene="dtd"
                     /locus_tag="PLV81_05710"
     CDS             complement(3317..3766)
                     /gene="dtd"
                     /locus_tag="PLV81_05710"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOM09631.1"
                     /translation="MRAVIQRVTRATVDVEGNIIASINKGLCVLVGFTATDTEKDLQY
                     ICQKIIGLRIFDDENAFMNLSIADVNGEILVVPQFTLYGDARKGRRPSYSLAMKPEEA
                     SQFFDTFVSLCKQQYPRVQQGKFQAYMQVFITNDGPVTILLDSSRLF"
     gene            complement(3770..4084)
                     /gene="trxA"
                     /locus_tag="PLV81_05715"
     CDS             complement(3770..4084)
                     /gene="trxA"
                     /locus_tag="PLV81_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015180335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOM09632.1"
                     /translation="MGNIVEVNDINFNSEVLEHPGKVLVDFWAPWCGPCRMQTPILEK
                     LAQDPATKTKIVKVNTDNNPETAQRYGISSIPTLILFENGKEIDRMVGVQPETVLKNK
                     LS"
     gene            4493..5215
                     /locus_tag="PLV81_05720"
     CDS             4493..5215
                     /locus_tag="PLV81_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOM09633.1"
                     /translation="MKSIINLFKFIWLFFWASFMSVVLFIPMTVAAIFSVTGNLAFNI
                     SKLWAWTMLIVTGVRVHIKGRETIKEGMQYVIISNHQSQYDILALVTSLKIQFRWVIK
                     KELLYVPLFGWALYAAKNVFIDRSNREKAIASINKAVNRLPQGVSLLVFAEGTRSKDG
                     TLQKFKKGGFTIAIERKMPILPVVVKGSRAILPKGSLIFHSGNIEVVVCDPIPADQYT
                     HETIEDLINKTHNVIEHELSVS"
     gene            complement(5628..6191)
                     /gene="thpR"
                     /locus_tag="PLV81_05725"
     CDS             complement(5628..6191)
                     /gene="thpR"
                     /locus_tag="PLV81_05725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOM09634.1"
                     /translation="MNRLFIALPIDEGIIQNLKPVYAMLSQYNDVLKVVEPSLYHLTL
                     KFIGECEGNVARAIESEFASLRFTIPPLPYVVKSIGMFPNSKNPSVIWTAIETNQKSI
                     NELVTCIQNFTKKYGIKEEDRPFVPHLTLARIRKGRKLTETIHKRLQTFATQEFGSAT
                     FPRVILFSSKLTPQGPVYTELQEINFA"
     gene            complement(6188..6484)
                     /gene="gatC"
                     /locus_tag="PLV81_05730"
     CDS             complement(6188..6484)
                     /gene="gatC"
                     /locus_tag="PLV81_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010277370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOM09635.1"
                     /translation="MRFDESVINKVCELARLELTAEEKQEFTTQLEDIIRYVEKINEL
                     NTASIQPADHIVPLSNVFREDIAKESLPVEDVEHIAPQFEKSHFVVPRIIEEIE"
     gene            complement(6484..7167)
                     /locus_tag="PLV81_05735"
     CDS             complement(6484..7167)
                     /locus_tag="PLV81_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09636.1"
                     /translation="MHNHITNIKVLSIVCVLALLLACKTQFGLVHTVKSLDELPVAVK
                     NLGDITVTIEPFTEETWVRIAAGSQFIDRSKSGRLPKIPFFLILIENKSSQPIILKEI
                     AITYGKTRSPMLTNDECKSYLSSPAYGFINVDAIITPQRLLAYSGKVQNIDFSSDVIP
                     CTLPFIPSGDIHCMVVAGKWIPVQERQFTVEITIKSPDNEKIVDCTFVRKEYRTKGNH
                     FLVPDGRND"
     gene            7319..9517
                     /locus_tag="PLV81_05740"
     CDS             7319..9517
                     /locus_tag="PLV81_05740"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOM09637.1"
                     /translation="MKRYIVIYSISIVLLCAACSSKPVVTKLLQTDLTVEYNLQLLNE
                     LYSDTVYVDPWLQKPWIVKVAHDIDKEKKWSKATRSLVIAATKQSAGKVLFPLQHGGK
                     LSFDCASMGQGRLIVQLLSPTKKKVLGEYSLSSLPFTHVQCNIPHSFADAKLVMEFQS
                     YSKDPAYCFVANAVVTHRDNDFKKPNVVFISVDALRADAVHCIIPKYNITPNMDALAA
                     DGAAFTRHFVVANWTRPSTIAMLSSVYGSATGVNIYYFQVSKQEKHYFYTQSGVVPLP
                     VLFGNNGYITRSIGNNAFILDHSGIGVDLGFDDMSEYERQWEDTMDIADEVIQWITAN
                     NDKPFFLFINFNAPHNAYIPPPQYLDPLRKSIKHVHPWFRRYLGEVAYTDDYIGRVVT
                     ALKKLNLYDNTIIVVTTDHGEVFNSVHEHSPYTDKKSIFTHGQTMYDEELHVPLIIKL
                     PKNIFSDSKAVPYQVRNIDIAPTVCELSDIPVPDSYQGKTLMSIIAGKELQDRPLYSE
                     GRLMYAVRIDNFKYVEKFYGFGIKPHHWGGAQVDEYYELYDLNQDPDELHNIVAVDTK
                     KAFEMQQKLYALRFKQPENILYAKKDVKGSISVREGFFYTVESSGVVKKISRQKYMFS
                     LKKGQRLVFSTIPSRTACYIQSDGSVALWGYSGTALPETDGMYVFDTTQPLLQKMPDD
                     AALRLFPDELIVWSKPQKGGIQKVSEHVAISDEMNTLLKQWGYIQNGGAR"
     gene            complement(9520..9873)
                     /locus_tag="PLV81_05745"
     CDS             complement(9520..9873)
                     /locus_tag="PLV81_05745"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOM09638.1"
                     /translation="MNNHQYGMQGETLAKDYVEKQGFSVIEQNFRYGRYGEIDIIGIK
                     DTLLVFFEVKTRHTLSYGNPSYAITNKKKKALRAVATYFLIQNPHYNSNQYTLRFDCI
                     TILGQTIEWIQDIVR"
     gene            complement(9905..11095)
                     /locus_tag="PLV81_05750"
     CDS             complement(9905..11095)
                     /locus_tag="PLV81_05750"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF023299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOM09639.1"
                     /translation="MAEMRKIPVDELKPGMRFDKPVYIDSNNMLVAANVSIKLDDIKK
                     LMRWGVSEVETAGNLVSTEQEIKAATARATMTESDEAKKIIDDYNKLLTQRVPLIEIH
                     KDACKAVGNVHNAIKADQQFAIDEIDVSLTKIMEIMKNYPNIFLFLYGLEEGKNYLVV
                     HSVNVTFYSLLIGMALKYTPLKLRELGIGTMLIDAGMIKLPAYIMHKQSNLTEHEFNQ
                     IKTHPLHGYKALRQLGKIPEKSALVCLQHHEQYDGKGYPRGLKGTQIDEYARIAAIAD
                     SYEAQIANRAYRKRVFFYQAMKNLLSSGINKFDPVILRIFLSRMSVYPIGSIVELSNG
                     TIGIVIGSIPQKPLRPIVKLIIDSNKKRFTDTIIVNLLEEKALYITRALDEAEIGVNL
                     FEVL"
     gene            complement(11170..11436)
                     /locus_tag="PLV81_05755"
     CDS             complement(11170..11436)
                     /locus_tag="PLV81_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HOM09640.1"
                     /translation="MENIAVALQYSVEEHIPKVIAKGKGKLAKSILDKAKEYNIPIYQ
                     DKDLVAVLSSLETGSYIPESLYRAVAVVLAYCYQINSDFRKKLN"
     gene            complement(11448..12422)
                     /locus_tag="PLV81_05760"
     CDS             complement(11448..12422)
                     /locus_tag="PLV81_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09641.1"
                     /translation="MKITDNIVSAHRTHSSAITLKSGQIVVGTITKRLTANTAMVRVG
                     NQIFETRFARGLPQSNHILLKFDTITGNRFIFSLVEPKALGNTNILSATPSNYALFMR
                     NLNAIDSLTVFSIHKALFDTNANREYTFTVIAPLLQRLLQKGVPERLLQKFCYTLSKD
                     TGISLTMHILQNIGDSSLQDFELHDDDIESLMSMMSKDDIESLVQYLGDSSGNVFTVP
                     LYDGNSFSDAYILSDDNYVCIDVTFSHLGKLNIMITGKDTIAATIYCFTDAAFSQLHQ
                     QCPIIARSIHEQTGKKAACSCIQRSDWEEKIIALINNMYIQYSIDFSV"
     gene            complement(12419..13072)
                     /locus_tag="PLV81_05765"
     CDS             complement(12419..13072)
                     /locus_tag="PLV81_05765"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOM09642.1"
                     /translation="MGAKPDFAIEQEECTKGYNIIAGIDEAGRGSLAGPLSVSMVIYP
                     AEIFSNPPEEILRYINDSKKLPYKKRIQAKDIITHYCLWYDCLLVPPEIIDEKNINGA
                     TYFAITTLLQQCPHTPHTVLLDGNFSFPLSLPYKSIIKGDCLSISIASASIIAKVTRD
                     EYMISINETYPEYGFAHHKGYATAKHIDAIKKYGPSPLHRKTYEPVARLFYELSLFP"
     gene            complement(13079..13498)
                     /gene="rplS"
                     /locus_tag="PLV81_05770"
     CDS             complement(13079..13498)
                     /gene="rplS"
                     /locus_tag="PLV81_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOM09643.1"
                     /translation="MKAIQLVQNEFSKSYRDVNFEVGDTVKVHYRIIEGDKERIQVYE
                     GIVIAIDNKGISKTFTVRRISYDVGVERIFPLHSPRIEKITVVRKGKKRRAKLYFLRE
                     RTGKSAKLKEVRSRKKAIETPATHEVVDNSENTETNA"
     gene            complement(13495..14196)
                     /gene="trmD"
                     /locus_tag="PLV81_05775"
     CDS             complement(13495..14196)
                     /gene="trmD"
                     /locus_tag="PLV81_05775"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOM09644.1"
                     /translation="MKIFKIITLFPEFFESPLQTGLMGKAVEKGLCRFEIINLRQWGE
                     GQYRQCDDYPYGGGSGMVLMPGPLFKAIELHKGDSTVIYPSPSGELLTQELVKKLFLY
                     DSYCFICGQYEGIDQRVIDRFVDYEISVGDYVLSGGEYATLTIIDALCRYIPGFMSNP
                     QSLLEESFENYLLEYPQYTRPREIDGMAVPDILISGNHEKIRQWRLEKSIEKTKAVRP
                     DLYRLYKAKKDEVEQ"
     gene            complement(14193..14711)
                     /gene="rimM"
                     /locus_tag="PLV81_05780"
     CDS             complement(14193..14711)
                     /gene="rimM"
                     /locus_tag="PLV81_05780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOM09645.1"
                     /translation="MSSKEYFRIGVISGIHGLDGCLKVHIITDFPERFSGDVPVYVNI
                     RGHYKAFTLTGFTFAAKRTGYMYLDGIHDVTAANAIKGCEIFIDSGDTEVTLEPDSYH
                     YSAIIGCTVYYNNTIFGHVTDIIQTGAADTLVLKTPEGKEYLIPFVESMVSTNRLHER
                     ILEIFPIEGLIE"
     gene            complement(14708..14944)
                     /locus_tag="PLV81_05785"
     CDS             complement(14708..14944)
                     /locus_tag="PLV81_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOM09646.1"
                     /translation="MNYKELVEYMVKSLVDHPDKVEIREIEGEKSTILELKVTKEDIG
                     KVIGKHGRIARAIRTILNASATKTGKRIVLEILD"
     gene            complement(14981..15217)
                     /gene="rpsP"
                     /locus_tag="PLV81_05790"
     CDS             complement(14981..15217)
                     /gene="rpsP"
                     /locus_tag="PLV81_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOM09647.1"
                     /translation="MVKIRLQRAGTKKKPFYKVVVVDSRKRRDGAVIESLGHYQPIVK
                     GEQFVVDREKVAEWLKKGAQPTETIAKLLKKAGI"
     gene            complement(15419..16087)
                     /gene="rpe"
                     /locus_tag="PLV81_05795"
     CDS             complement(15419..16087)
                     /gene="rpe"
                     /locus_tag="PLV81_05795"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOM09648.1"
                     /translation="MTDTILLSPSIIATDLSTLGSVVPTFDHSCMHFLHIDVMDGSFV
                     PNITIGPGYIQNLKQHTDIPLDIHLIIEKPELSLQSFIDCKPYFLTIHFESTRQPVRL
                     LQSIKNCGIKAGISINPATPVEQLFDILAYTDMVLLMSVEPGFYGQTFIPSSLKKIEK
                     LNTFIRTNNCNTLIEVDGGINEDNIADVVKAGANVIVAGSSVFKSSDINKQARKLLEI
                     AKKG"
     gene            complement(16080..17108)
                     /locus_tag="PLV81_05800"
     CDS             complement(16080..17108)
                     /locus_tag="PLV81_05800"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOM09649.1"
                     /translation="MPRYSLKDVIERSKGWLKHPYSRIAFIIFIAISLYLIVSAIIVV
                     VLTKPEKEVKIPDVVGKRYADVHNSLIRKGLKPQLKFYDVHDIDNDIILRQYPEPGEI
                     VSENSTIRLLVSRSNLTIDMPSVVGMELPFALNKLKNLHLYDKTISLRVGVISYIPSE
                     KTADNVVIDQSPKSGEKIVLDRKVNLLVSSGSATQPVMPKVVGQSVDLCFPLLMSKKV
                     FVSFTVLPTSDIAQSGIIASVTPLEGQPITEGQTVTIQVYYYEMKDKPYYAYERLKYT
                     VPGDENEGLYEIYVSDNTSKRLCFSQNLKPGNTIDCVFHRTGNARVYVMCNKKQVKVL
                     SFDVEEFD"
     gene            complement(17280..18731)
                     /locus_tag="PLV81_05805"
     CDS             complement(17280..18731)
                     /locus_tag="PLV81_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOM09650.1"
                     /translation="MNPLLIIKILSFLIIIVSSFMTIPAGIALYHGEFHSFHAFAFTI
                     SVVILIAATILILLRHKKVEILTTRDGFLLVTFSWISASIIGALPFYISGAIPSYTDA
                     FFETVSGFTTTGASILTDIESLPRSLLFWRSLTHWLGGMGIVVLAVAILPMMGIGGLQ
                     LIRAEAPGPTVDRISPRITKTAKTLWLLYIGFTILETLLLMIGGMDLFDSLSHTFGTL
                     ATGGFSTKNASVGHYNSAYIDGVITVFMLIAGINFSLHYRLLTGRFKPLIRDTELKAF
                     LFIFIASTTLIALSLYGTTYQTIGECVRFASFQAASILTTTGFATADYEQWVPFAHTI
                     LFILMFVGGCSGSTGGGIKVVRLVILLKQALNEMKLLIHPRGVFALKISGNMVKKNIV
                     YAVMGFFFLYIMILLTVTFVIAFDGHDITTSFTTALATLGNIGPGFAKVGPTKNYAFY
                     ESYIKWVLSIAMIVGRLELYTVLVICMPHFWKR"
     gene            complement(18747..20090)
                     /gene="trkA"
                     /locus_tag="PLV81_05810"
     CDS             complement(18747..20090)
                     /gene="trkA"
                     /locus_tag="PLV81_05810"
                     /inference="COORDINATES: protein motif:HMM:NF007031.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOM09651.1"
                     /translation="MHTVILGAGVVGYQIAEQLIAEGKDVVILEKNPERAKFISEHLD
                     CMVINDDGTNVSSLKRAGTKDASIFISVTNSDEVNMIACGLVASEFNVPLKIARVRNI
                     DYSKSKIMGKSFLGIDLIVNSEVETARQIANTIALGADSDVMFFENTDLQMRNIVVTR
                     GSYFANKTVKDIRKGISEPFLISGIMRNNDFLIPTGDTIVREGDNIYLMATQKNFTRI
                     FIQVGKKQEKIDRIVIIGGGRVGSLACEYLIRTGRKITVVETDYERCKFLADKFPDAL
                     ILNADISDESVFHEEEIAKHDLIVTVTDNQELNMLISLYAKSMGIKRSIALVTNSSYL
                     AIASRLDIDVTVNPKLSTVDAIMKFIRRGNIKSLHSIFHGRAEVIEFSVDEKNPLVGK
                     PLKEMLFPKNSIILSVVRNNTNILPHGNIVIEPNDLVIIIAEKTSIPDLEQFIQG"
     gene            complement(20262..20468)
                     /locus_tag="PLV81_05815"
     CDS             complement(20262..20468)
                     /locus_tag="PLV81_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HOM09652.1"
                     /translation="MGRLLILAGILLILTGILFQVFPKFLGKLPGDVMYSKGNMTVYF
                     PFMTMLIISIILTLLVNVFLRFFK"
     gene            20787..22649
                     /locus_tag="PLV81_05820"
     CDS             20787..22649
                     /locus_tag="PLV81_05820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM09653.1"
                     /translation="MEHSIDKNFRIKELIDNLPDIVISVDYPSGIIQYANVNAIERLG
                     FSIKRAKTLRDLSYFVIPKYRRKYLQHWRDIKDGILIPSLVFEVKTIDGRIIWVEQKN
                     IYIKQGDSVIRIHAVIRDITERKRLEEEMLQAFYFSENLFLHSPYIILLYDNEGYIKR
                     QNMTFRAIAEHVDNMIPNYNVWKDKQIQKYGIDLIFKEVMRGRIMDIPRFPYTVKIKG
                     EDVTFYFAGKAFPYMRSSREPEGFVVMLEDVSARQKREEETLETKRLESVGRLARGIA
                     HEFNNILAGISGFAEILLRKLSPDDPNRHFATRIFEAASKAATLTNQLLGFARGQLYN
                     PSYLDIRAPIMYAIGATPNLHNRITIKKEFEESEYKVYGDVNQLRQVFIEILRNAAEA
                     IGTEGEIEIKVTYVSNEFLPAEIRKKYIKCVQVDIIDSGTGIDDSIIPKVFEPYFTTK
                     DKLKHTGMGLSIVFGHIKNHEGHITLSRAEKGTKVTILLPAIKEPDLAILKQPRYQRI
                     LVVDDNNETCLLLRSYFEDSGALVTISNNGLSAVEMMQNAPNRFDCIFLDLILPDING
                     KDVLNRIRNFNAHVPVFIMSGYDMSSFISEHGSDQNLFFLMKPFNLEDLDVLLK"
     gene            complement(22653..23660)
                     /locus_tag="PLV81_05825"
     CDS             complement(22653..23660)
                     /locus_tag="PLV81_05825"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOM09654.1"
                     /translation="MQGINSTPTVMEIITTLPQERTGIDRIVGNNKTIISLKNKIKRI
                     AGSKATVMILGESGTGKELIARAIHEESQRKHNQFIPVDCSTLTETIIESELFGHIKG
                     AFTGADKNKKGLFEEANGGTIFLDEIGNLQPHIQSKLLRFLQEREIKPVGSSKIVKVD
                     VRIISATNINVDEEISQGNFREDLYYRLSAIELHIPPLRERRDDIPVLAEHFIHMYSH
                     DLQKTIIGIRDDALELLSNREWKGNVRELQNVIEHAMIVEDNTILSKETFMQILPEYK
                     EGISQELSPLNLFRAIEEFEKKHIIYVLSLAHHNKSRAAKMLGISRSVLYEKLKKYGI
                     H"
     gene            24028..25089
                     /locus_tag="PLV81_05830"
     CDS             24028..25089
                     /locus_tag="PLV81_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOM09655.1"
                     /translation="MISILLMSDIHFGSDKALPYIDDSVRFATFQKICMIARQHSIFI
                     IAGDLFEGTIAQNPYYSSIKELLQSLVQEGVKIIYIPGEAELEDNNNIDVIHEIPSTV
                     TLFGRSKNYFDLVLDNEIVYCYGGDSDIASITKASQEGFHIGIFHIDLEENNHNTVDS
                     HVISVKLLKSMPLDFYALGHHHNFSMYKYKNKIIGAYPGSPEAVAPGETGDRYVLSIT
                     VMNNEIHQIKRITVNSLRIESLTIQCNSIKSNDELHHILKPYNKPSIVLHCTLEGVRS
                     FDSNHIVALCDRCAGVIFQDNSKLSGEFICAKYKDEKTLKGYFFTELQKNFDNVAGDI
                     MLDIINDIENTCSTYKGDL"
     gene            25086..27179
                     /locus_tag="PLV81_05835"
     CDS             25086..27179
                     /locus_tag="PLV81_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09656.1"
                     /translation="MKLVRSIVSKIGFAESKQTDFNEGIHFIFGKNNSGKTLISKSFI
                     DTIYPQNPSLIDSEAWDTMFATFTLECSQTKVEIQRKGNKEIKIFEITSNGNTQNEDT
                     YPLSFNPGSLSVNCLSKLHGLFNKDTFAILCYIPSPLDTQYYSKDFSDSMKSVLLLDR
                     SLYYYAFSRLFTDTINKTGNYQAHVLAEIQNKIKHCKTKLDLLQLKEQKQKKHRAEIQ
                     EVNERITSYTNDINELRKKIEACNRYMDTLVAIDNVKKQIADVNGQIEALEEKLETIE
                     KYTKLIASKYPQFKDFSSEKRNNIHAMQNIYRQIQAISEEIETIIFGQESKNHALARN
                     LLIINIILALGVLFVFLKPVFGITSMHRWLLVSLLILIQISSIGYYLIYVMMHSFKKI
                     VLPLQQQKETYQIKLETLLNQNGISLDGITIDEIYEFLLQYFEEYNEYNEKQIDLLTI
                     QKEIDESTSIDILAEEKRKLESNVKMLSDELHNLQEDIGIAIESAEHCMTIKMSLHNE
                     LDEKKALLVESQNKLDNIPEEIIDYEDEKNSILQEIDDLEKELKTINEKDRLINIIIT
                     SFDTAMQRYFKDRFNILTGKIKIMLQDLTQNKFGKIDEEYIKKIITHSINTPNPSLKY
                     CIYVAAIFALYELLQQELPNIHLPLIIDDPFVLMDEETMGEFIKILQLWVGNRQVIIF
                     THNSNIKKLCAVTEL"
     gene            27182..28174
                     /locus_tag="PLV81_05840"
     CDS             27182..28174
                     /locus_tag="PLV81_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09657.1"
                     /translation="MDKNGQLTFFDDPLLQGINEAYTFIEAGDFTQAVKKIDDLMTIN
                     PDYPGLAEAYRVARFWESRKKAMQELQKGKDTADFLMTQWEEFEAFAQNRGLVWATAY
                     QKAMRFVFFTASEHYTIAFNDRENPTDNLQLLMQLGTCFIRLGEYQHTIEALEYARSA
                     YRSNACILSLLAESYYHTGDVPRALLYFREAFLLNPSEIDIQLLSAKPIIEALELVKQ
                     HKPDCSDVREWVPVMAHIHDIFYVKRQLNSSQLETIKKDIVKLEASFQTLDKEKIAQS
                     NIVPRLINRYLWMLDYFEFQQYDQKSLFDIHDRLLAIDKGLFESYFQKKTFSKH"
     assembly_gap    28461..28560
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <28561..30528
                     /locus_tag="PLV81_05845"
     CDS             <28561..30528
                     /locus_tag="PLV81_05845"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOM09658.1"
                     /translation="MLGDTAVAVNPNDSRYAHLIGKRVILPLVNKPIPIIADEFVDPS
                     FGTGLVKVTPAHDPNDFEMGKRHNLEEVNIFDKNAIVNENGGVYKGLDRYQAREKVLE
                     DLKALGLLEKIEDHNHAVGHCYRCHTVIEPYLSKQWFVKIQPLAQEAIRVVEEGKIRF
                     VPQQWTKIYFEWMYNIKDWCISRQLWWGHRIPAFYCKDCGHIMVELDDPTVCSMCNSK
                     NLEQDPDVLDTWFSSALWPFSTFGWPQKTKSLEKFYPTSVLVTGFDIIFFWVARMIMM
                     GCKFMNDVPFYDVYIHALVRDEHGQKMSKSRGNVIDPLIMMDKYGTDAFRFTLAIFAA
                     QGRDIILSEKRIEGYSAFCNKIWNSTRFILMNLGNDFKAEAIDINSLDMFDKWILHAF
                     NQTVKKTTQALVEYRFNDAAQTLYDFWWHEFCDWYIELSKPRIYAGDSSTGTIAKQVL
                     YFVLHEYLKLLHPFMPFITEEIWSNITSGEGRIVVAPWPQYDERFNFEHEFNNAELFK
                     EIVYKIRNIRGEMNVPPDKKAHVVIKAGKKIEQFIQQQEHNLKILAKLEAVECNADYT
                     PQKTDASAVLPECELYMPLKDLIDLDKEKARLDKEIARIQGELDKVMKKLNDSTFLEK
                     APAHIIEKEQNKKAELEEVLGKLTTGREKLG"
     gene            30593..31513
                     /locus_tag="PLV81_05850"
     CDS             30593..31513
                     /locus_tag="PLV81_05850"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOM09659.1"
                     /translation="MHRRKSIVLIIASLFVYVSCSSSLLNFFRWYERSKAGLTEKTVM
                     IDGHTVHYLEGGKGDTVLCIHGFGGTMDHWTRFARYVTDAYRVVALDLPGFGDSSFKE
                     GGDYSIAKQVERVHEFAHTLKLQKFHIVGNSMGGYIAGYYAITYPDDVITLGLFAAAG
                     VKSPNESELAKELKKGNNPLIVEDVESYDRLMEFVFYKPIWLPGFAKQYFATQAVAKK
                     THFQYIFKQIHDVTLLEKYLGNIHAPTLLLWGENDRVLDVSGAYVFKEKIRNAQLVVF
                     KECGHLPMIEKPEESATVYKRFLKGQKVEL"
     gene            complement(31508..31963)
                     /locus_tag="PLV81_05855"
     CDS             complement(31508..31963)
                     /locus_tag="PLV81_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09660.1"
                     /translation="MKIKSALINRFVELIEENHQIITEQFMNNLLRNPDTIAFRKLDR
                     QLIYESADNLYRELSKWIARDYPKEEIARYYRRIGHERFEQGIPVSQACKALILQKRH
                     LWLFVLDKLYDDTTAYKEALALNNRVVLYFDRATFAMLQGYEDMLYKKL"
     gene            complement(32043..33086)
                     /locus_tag="PLV81_05860"
     CDS             complement(32043..33086)
                     /locus_tag="PLV81_05860"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOM09661.1"
                     /translation="MKHYTKLPIVFLLIITVYSFVYAQLPIEVYFTTPEKEEQTTAIQ
                     KALLQKIHSTKTSIYAALFEITDPLIIDALCKAKSNGIDVRLVLESDRLKEKVKTKLE
                     EANIPLRLDERKGFMHNKFFIFDNKAVWTGSYNITEREAKHNNNNAIYIENEEIASIY
                     LAEFNEMFVQAIFGNRTEYTPFPFLSNKYYVKINDIDINVYFSPDDDIERIITKRIEK
                     AKKSILFLAFSFTSDAIGEAIIAKHKQGIAVKGVFEKRDIKNVNSEYIKLKVEGLDVL
                     YDANPKTMHHKVIIIDDEWVITGSYNFSKNARKRNDENCIMIRSSEIAKLYAQEFEKI
                     YSQAYRAKTKKKK"
     gene            complement(33083..34009)
                     /locus_tag="PLV81_05865"
     CDS             complement(33083..34009)
                     /locus_tag="PLV81_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09662.1"
                     /translation="MSTPIVEEKTPKGYTLKINIAKKQYIAFNPKGEIIYSDDNKTKP
                     FHIMKTIVFIGLEPVTSTRNHPYQLLKIQNNALIISYNAGEGKRQYLFFPSDTLLDMV
                     PCITEFFNDTASFVSYVKKGNVPDYIIVNSTFSNNDITFISSISPSSRLEIVNTTSAV
                     TIDDTKRAIDQMNNVDINLLSQNPVFLARIHLRNLDLVRVKQIILDMDITESDAQYII
                     AFLSTMIKNSDKEELQKHVEKLKELKKTYEFYFHLITHNDEKIQEFITQASKNDLITF
                     QTLIQKAKLLFPTKEASLRFVEYENLIWEKQQ"
     gene            34272..>35674
                     /locus_tag="PLV81_05870"
     CDS             34272..>35674
                     /locus_tag="PLV81_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOM09663.1"
                     /translation="MNTINILSPVRYPGKIHAVSSSFETHEADNVLHNDESFWCTAKS
                     HNAVSEFIIIDYGSIVAVNIVEIYPSPSGKTTFPSEFRLECSMDGINWEVLKAENKFT
                     LDEDAYRLLLPITVFQYLKVYIIKPKRIAAKYFAEIGKIQAGIQGAVSITASSSSSYE
                     HDPSNLMEIDNQKYWESEITSSASRQWIEVDFGMIFPMTNIGIIAPQNPALFPEQFSI
                     EITEDKKIWTTLTDVKRFKAQPNGSYMFGGYQFNARYLRINIMTVQTEKQKYCAQIAG
                     IFAHAALPALRHFHINAAVQYATVFQPGIVKLAADGDTTPGAVVQASDPRLQDASTIF
                     KGIVQLADDGSNEEGLVVQASDSRLAPATELKPGIVRLAYDREVAEGAVVQSTDSRIK
                     EATDTTFGIVKLCPNGQYINNAVVRGDDSRLQKATTKDYGIVRLAENGEDNEFCVVQG
                     NDRRLKDATIVSKGIVEL"
BASE COUNT        11028 a         6268 c         6885 g        11293 t
ORIGIN      
        1 tgtgctgata caaaaaaaga aactattacc gtgttccatg ctggcagcct atcggcactg
       61 tttaaagaat gcaaggttac atttgaaaaa aagcataact atactctttt gctggaagct
      121 tcgggtagtg ttgatgcagc acgaaaatta accgatttac ataaacagtg tgatgtgatt
      181 gcactggccg atgttgagct ttttgatagc atgcttaaaa gctattgtcc atactggatt
      241 gcatttgcag ggaatgagat ggtgcttgcg tatactgaaa agagtaaatt tgcacaggca
      301 atagcaacaa actggatgga tgcattttta cggtatgata ttatctgtac acgctctgac
      361 cccttacggg acccctgcgg gtatagggca cttctggtat ggaaattagc atcacaatat
      421 tataaaaata cagagcttga aaaaatcttt gccatgcgtt cacctcttgc gtatatgcgc
      481 ccaaaagaaa ttgattgcat tgcattgctt gaaacaggcg cctgtgacgc gatgtggata
      541 tacaaatcag ttgcggtaca gcaaaatttc ccctatataa cgctggatga gcatataaat
      601 ttaggcagtc atacatacga gcatatatac aggaaagcct gtattacgct gtcagacaca
      661 aagaagcagt atacgttttg cggaagcacc atcacctatg gcgcatccat tccagttact
      721 gcaaacaatg taaaaggtgc tattgccttt ttatcgtttc tttgtagcaa agaaggacag
      781 ggattaataa ataagcacgg atttacatct atagtaccgt atagcaataa tagtgaacaa
      841 ttacctgtgg aacttaagaa ggtggttgca ggtggtaacc gtaaataaaa aaaatattgt
      901 gttttacagt atagttgttg tgctgtcgtt tgtggtagca ttccttgttg tggtgcccat
      961 tgcttcaatg gtgctttcag tatcagtacg tgatgttgta gacatacttt ttgataggga
     1021 agttgtgcaa tcgcttcttt taacctttga atgtgcattc tatgctacgt tgattgcagt
     1081 actttttggc ataccccttg cctatctctt tgcctttact cattttccgt tgaagcgttt
     1141 aattatcacg ctatttgata tacccatgat cattccccat accgcaagcg gcatagcatt
     1201 actttttgta tttggaagtg gctttctatc acatttattt aagtcgctac acatgtcatt
     1261 tgttggtagc aaaacaggca ttgtcatagc catggctttt gtttcaatac cgtattttat
     1321 caatgcagtg caaaatggtt atgcctcaat tgacaggcgc cttattaatg tagcacgaac
     1381 gttaggtgct tcacagacgc agtgctttgt ccatattata ttgccacttt cagtcagggc
     1441 tatcattact ggtagtctta tgatgtgggg acgaggcatc agcgagtttg gcgctgttat
     1501 gattattgct taccacccca tgattgcacc tgtccttttg tatgacaggt actcagcata
     1561 tggattggaa ggttcacggc caattgctgc actgctcata gttttttgca tagtgttttt
     1621 tattcttata cggagtttac ttgcttacag ggaaaaacat gcttacacta cataacatac
     1681 aatgggaaag taaacagttt acactgacaa ttccatcatt gcaatttcat gatggagctc
     1741 atgccatgat catgggctct tcgggttcag ggaagacgtt actgttgtcc atcatagcag
     1801 gaattattgc tccccatcat ggcaccatct atagtgacaa tgaagacatt acgcatatcc
     1861 cccctgaaac acgtggcttt tccattgtgt atcaggaacc agcgttattt gcccacatga
     1921 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngaactcatt ggtattttga
     2041 accacataaa aaaagaatat aatcctacag tgctgcatgt gacgcatgat tttgatgaaa
     2101 cgctgcagct tggcaattac ctgtatatca tgagtaaagg tgcaatcatc cagcatggta
     2161 ctgttaccga tgtgtatcgc tatcccagtg atatttttgt agcacagatg gttggagtaa
     2221 aaaatatttt tcatggaagc gttgtacata aaaaagatgg tgcgtatttt gtaacggata
     2281 acggcattgc atttttttta ggcgatagtt atcgcaaatt tttccagtat gcaatactca
     2341 gggctgagga tataatcata tccaggaagc tgttgcatag cagtgcaacc aatcagttta
     2401 aaggaactgt tatagatgtt actgaggtga agggcttata taaagtaagc gttgatatag
     2461 gtgaaatagt acaatcaatg atcaccaaac gatcattaca agcaatgaac ataaaaaaag
     2521 gtgcaaaagt tgttgtaatt tttaagggca gtgctataca cttgttttaa atgtgatttg
     2581 aattatattg atactatcaa aagcaatctt gttaattgta gtgaatgtac gatatcctaa
     2641 ttgtgaatag atgacatagc agcgagtgtc aggcatgctt caacaggttc agcagatgat
     2701 ttgatgaaca tgttgcgata gttgtagtac ttagtcaaag actaacgata taatttttca
     2761 aaattataat agcattacca cgagggatga attgaagaga ttatttaata gctttatggt
     2821 tactacaata ttgtgcttta tttcatcggt aactatcgca cagcatatag aagaagctgt
     2881 acagctcaat accaaaggtc ttttttactt agaatgcggt aattatgaaa aggcgtatga
     2941 atattttatg ctggcactgc agcacgatcc aaccaataag ttattatata ataattgcgc
     3001 tgtagcatgt atgaacatga aaaaatatca ggaagcatac cgtatgctct caatagccct
     3061 tgcaatagat ccttactatg tcaaggcact atccaatatg gcaattgtca attttcattt
     3121 attgaagttt gcagatgcat acacatatta ttcaaaagca ttgatggtcg atagctcata
     3181 tacaaaagag cgctttagat tggataaggt tattgcaggc gtaaaaaaag ttcaaagcga
     3241 aaatcccgaa aacgatgacc ttaaagaaat tgtgcgtcag ctggagatac taaaaaacaa
     3301 taaccaggat atatattcaa aataatcttg aactatctaa tagtattgta acaggaccat
     3361 cattagtaat aaaaacctgc atgtaggctt gaaatttccc ctgctgcact ctgggatact
     3421 gctgtttgca taatgaaaca aatgtgtcaa aaaactgtga agcttcttca ggcttcatgg
     3481 caagagaata tgaaggtctt cggccttttc gcgcatcgcc atacaatgta aattgaggga
     3541 ctacaagaat ttcaccatta acatcagcta ttgataggtt catgaaggca ttttcatcgt
     3601 caaaaatacg aagacctata attttttggc atatgtactg taaatctttt tcagtgtcgg
     3661 tggcagtaaa accaactaaa acacacaatc ctttgtttat ggatgcaatg atattcccct
     3721 caacgtcaac agttgcccgt gttacccgct gtatgactgc acgcattggt tatgaaagtt
     3781 tatttttaag tacagtttca ggctgaacgc ctaccatacg gtcaatttct ttgccatttt
     3841 caaaaagtat gagagttggt atagatgaaa tgccgtaccg ctgggctgtc tcgggattat
     3901 tgtcagtatt gacttttact atctttgttt ttgttgcggg atcctgagct aatttttcta
     3961 aaattggtgt ttgcatgcgg cagggaccac accatggagc ccagaaatca accagcactt
     4021 tgccaggatg ttcaagtacc tcagaattga aattgatgtc attaacttca acaatatttc
     4081 ccatacatac ctccacaaaa attcaagaaa caataatagc atggtaaaac gttaattcaa
     4141 caattatttt aaagatagta cactaattat tttttgaaaa attatataag ttaatgcaat
     4201 atccagtctt tccgccctta gctatggatg ttgtatacgt tactttcatg tatacatgac
     4261 tattggttct ggatgtttat atatatagat taatacacca ttgcaattat tgtcaacaca
     4321 aaagaatgaa caataaagat atcccatgat ttttttactt gcagttaaca ttatgttgtg
     4381 gctatttact aaaacattta tactgagtct atgatatcct tttgaattga caatgaaaat
     4441 gtatattgta taataatata ataaatgaaa ttttgttttt tggtatttgt ttatgaaatc
     4501 tattattaat ctttttaaat tcatctggct tttcttctgg gcatcattta tgagtgtggt
     4561 actttttatc cccatgactg tggcagcaat attttcagta actggaaatc tggcatttaa
     4621 tatttcaaaa ctgtgggcat ggaccatgct gatagttaca ggggtacgag tacacattaa
     4681 aggcagggaa acaattaagg aaggcatgca gtatgttatt atcagcaatc atcaatcaca
     4741 gtatgatatt cttgcacttg ttacttcttt gaaaattcaa ttcaggtggg ttattaaaaa
     4801 agaattgctg tatgttccgc tctttggatg ggcattatat gcagcaaaaa atgtgtttat
     4861 tgaccgtagc aacagggaaa aagccatagc cagcatcaat aaagcggtta accgtttgcc
     4921 acagggagta agtcttcttg tttttgctga aggaacacgt tcaaaagatg ggacattaca
     4981 aaaatttaaa aaaggtggct ttactatagc tattgaacgc aaaatgccaa ttttgccagt
     5041 tgttgtaaaa ggaagcaggg caatacttcc caaaggcagc cttattttcc actctggtaa
     5101 tattgaggtg gtggtgtgtg acccaatacc agcagatcaa tatacccatg agactattga
     5161 agatttaatt aataaaactc acaatgttat tgaacatgag ttatcagtat cataacttaa
     5221 aaccaccatc cagggttcaa caaattcata tgagggtgaa atcaatttcc aaataactag
     5281 attattcagg cattccaata aaatcaggac atctttagaa tgatacgtgc gattgcttca
     5341 tcctgacttt gtcgtgattt gtaatgactt tggaagcata tcaatgcgag cgaagcgtgg
     5401 cagtatcaaa taaaaggtaa ggagtatgta taaaaagggt agatttttag tccgctgaca
     5461 tgtcctcaaa atacgatgac agatagaaaa atacttcctt gctatgaggt tactgaattt
     5521 ataaaaggta tgaatttaaa cttttaactg atagtatcct ggtatttcaa taattcggga
     5581 ttcctcagga tggtatttta taatgaaatc tattctcaag gatgcattta agcaaaatta
     5641 atttcctgta actctgtata cacagggccc tgcggggtaa gcttggatga aaaaagtatt
     5701 acacggggaa acgttgcact accaaactcc tgtgtggcaa atgtttgcag gcgtttgtga
     5761 attgtttcgg tgagcttacg accctttcgt atgcgggcaa gcgtcaggtg tggcacaaaa
     5821 ggacggtctt cttccttaat gccgtatttt tttgtaaaat tctgaatgca ggtaacaagt
     5881 tcattgatgg atttctgatt ggtttctatt gctgtccata taaccgatgg atttttactg
     5941 tttggaaaca ttcctatact ttttactaca tagggtaggg gtggaatggt gaaacgtaat
     6001 gatgcaaatt cagattcaat tgcccgtgca acattgcctt cacattcccc aataaatttc
     6061 aatgttaaat gatataacga aggttcaaca acttttagta catcattgta ctgtgaaagc
     6121 atagcatata cgggttttaa attctggata attccttcgt caatgggtaa tgctataaaa
     6181 agcctgttca ttctatctcc tcaataatgc gaggtacaac aaaatggctt ttttcaaatt
     6241 gtggtgcaat atgttcaacg tcttctactg gcaatgattc tttagctatg tcttctctaa
     6301 acacattgga taatggaaca atgtgatcag caggctgtat actggctgtg ttaagctcat
     6361 ttatcttttc aacataacgg ataatgtctt caagctgtgt ggtgaattcc tgcttttctt
     6421 cagcggtcag ctctaaacgt gctaattcac atactttgtt aatgactgat tcatcaaatc
     6481 tcattaatca ttcctcccat ctggaactaa aaagtgattg ccttttgtcc tatattcttt
     6541 acgtacaaat gtacaatcaa ctattttttc attatcagga gactttattg taatttcaac
     6601 ggtaaactga cgttcttgca caggtatcca tttacctgct actaccatgc agtgtatatc
     6661 ccctgatggt atgaaaggca atgtacatgg aataacgtcg cttgaaaaat caatgttttg
     6721 cactttgcct gaatacgcaa gcaatcgctg tggagttatt atcgcatcaa catttataaa
     6781 accatatgct ggtgaagaaa gatagctttt acattcatca ttggtcaaca ttggggagcg
     6841 ggttttgcca tatgttattg caatctcttt aagtatgatt ggctggctgc ttttgttttc
     6901 tataagtatt aaaaaaaagg gtatttttgg tagccttcca ctttttgacc tgtcaatgaa
     6961 ctggctacct gcggcaatcc gtacccaggt ttcttcggtg aatggttcta tggtaacagt
     7021 aatatcacca aggtttttta cagcaacagg caattcatcc aatgacttta ccgtatggac
     7081 aaggccaaac tgtgttttgc aggccagtaa aagagccagc acacatacta tagagagtac
     7141 ttttatattg gtaatgtgat tatgcatggt atgcaaacaa taacagggtg atagttcatt
     7201 tgtaaagttt tttacacata atcacgtaag aaaattatga gatagtaaat caaaattata
     7261 ttgcaataat actggatggt atatccatgt gtttgtaatt atacgttact ggggaatcat
     7321 gaaacgatat attgttatat atagtattag catcgtgttg ctttgtgctg catgcagcag
     7381 taagccagtg gtaacaaaat tgcttcagac tgatttaact gttgagtata atctacagct
     7441 tctgaacgaa ttgtattctg atactgtgta tgttgaccca tggctgcaga agccgtggat
     7501 tgtgaaagtt gcccatgata tcgataaaga aaaaaaatgg tcaaaggcaa cacgttcact
     7561 ggtgattgct gcaacaaaac aaagcgcagg aaaggttttg tttccactgc aacatggcgg
     7621 aaagctttct tttgactgtg catcaatggg tcagggcagg cttattgtac agttactatc
     7681 gcccacaaaa aaaaaagtat tgggtgaata tagcctgtct tccttgccat ttacgcatgt
     7741 acagtgcaac atacctcatt catttgctga cgcaaagctt gttatggaat ttcaatcgta
     7801 ttcaaaagat cccgcatatt gttttgttgc caatgctgta gtcacgcata gagataatga
     7861 ctttaaaaaa ccaaatgtag tgtttatttc cgtggatgct ttgcgcgccg atgcagtaca
     7921 ctgtattatc ccaaagtaca acattactcc taacatggat gcactggcag ctgatggtgc
     7981 tgcgttcacc agacactttg tggtggcaaa ctggacacgc ccttcaacta ttgccatgct
     8041 ttcgtcggtt tatggttcag caacaggtgt taacatttac tactttcagg tgtcaaagca
     8101 ggaaaaacat tatttctata cacaaagcgg agtggtgcct ttgccagttc tttttggtaa
     8161 caatgggtat attacgcgtt ctattggcaa caacgcattt attctggatc attcaggtat
     8221 tggcgttgac cttggctttg atgatatgtc cgagtatgag cgtcaatggg aagataccat
     8281 ggatattgct gatgaggtga tacagtggat cactgccaat aacgataaac cattctttct
     8341 ttttattaat ttcaatgctc cacacaatgc ctatatccca cccccgcagt atttggatcc
     8401 acttagaaaa tcaattaaac atgtgcatcc gtggttcagg cgatatttag gtgaggttgc
     8461 ttacactgat gattatattg ggagggtggt gacagcgttg aagaagctaa acctgtatga
     8521 taataccatt attgtggtta ccaccgatca cggtgaagtg tttaacagcg tacatgaaca
     8581 cagcccatac actgataaaa agtcaatatt tacccatggg cagacaatgt atgatgaaga
     8641 attgcatgtg ccattaatta tcaagctgcc aaaaaatatc tttagtgatt ctaaggcggt
     8701 gccctatcag gtgcgtaata ttgatattgc gccaacggtg tgtgaattaa gtgatatacc
     8761 agtgcccgat agttaccagg gaaaaactct tatgagtatt attgcaggca aagaactgca
     8821 ggacaggcct ttatacagtg aaggcaggct catgtatgca gtcaggattg acaatttcaa
     8881 gtatgttgaa aaattttacg gatttgggat taagccgcat cactggggtg gggctcaggt
     8941 tgatgagtat tatgaattat atgatttgaa tcaggatcct gatgagctac ataatattgt
     9001 ggctgttgat acaaagaaag cgtttgaaat gcagcagaaa ttgtatgcac tacggtttaa
     9061 gcagccagag aatattctgt atgctaaaaa ggatgtcaag ggtagcatca gtgtccgtga
     9121 aggatttttt tatacggtgg agtctagtgg cgtggtgaaa aaaatttcac ggcaaaagta
     9181 catgttcagc cttaaaaagg ggcaacgcct tgttttttca actattccat cacgcactgc
     9241 ctgttatatt caatctgatg gcagcgttgc gctgtgggga tacagtggca ctgctcttcc
     9301 tgaaactgat ggcatgtatg tctttgatac aacacagcca ctgttacaga aaatgccgga
     9361 tgatgcagcg ttgcgtctat ttcctgatga actcattgtg tggagtaaac cacagaaggg
     9421 tggcatacag aaggtatcag aacatgtagc catttcagat gaaatgaata cgcttctcaa
     9481 acagtggggt tatattcaga acggtggtgc gcgctaagtt caccgtacta tatcctgtat
     9541 ccattctatt gtttgtccta atatggttat acaatcaaat cgtagagtat actggttact
     9601 attgtagtgt ggattctgaa taaggaaata ggttgccacg gcgcgcagtg cttttttctt
     9661 tttattggtg atagcatatg acggattacc gtaagataat gtatggcgtg ttttaacctc
     9721 aaagaacaca agcaatgtat ccttgatgcc aatgatgtca atttccccat agcgtccata
     9781 gcgaaaattc tgttcaataa ctgaaaatcc ctgtttttca acataatctt ttgcaagggt
     9841 ttctccttgc atgccatact ggtggttgtt cattggtata ctccgctatg tatctatagt
     9901 tagattacag tacttcaaaa agatttacgc caatttcggc ttcatcaagt gcccgtgtta
     9961 tatatagagc tttttcttca agaaggttaa cgataatggt atcggtgaac cgttttttat
    10021 ttgaatcaat aatgagctta acaatgggac gcaatggctt ttgtgggata gaaccaataa
    10081 caattcctat ggtgccattg ctaagctcaa ctatggaacc aataggatat actgacatcc
    10141 ttgacaggaa tatacgcaat ataacaggat caaatttatt gattccgctt gaaagcaaat
    10201 tcttcattgc ctgatagaag aatacacgtt tgcggtaagc tctgtttgct atctgggctt
    10261 cgtatgagtc agctattgca gctatacggg catattcatc aatttgtgtg ccttttaagc
    10321 ctctggggta tcctttacca tcatactgct catggtgttg caggcataca agggcacttt
    10381 tttcaggtat cttccccaat tgtcgcagtg ctttatatcc atgcagagga tgggttttta
    10441 tctggttaaa ttcatgctca gtaaggttgg attgcttatg cataatgtat gcgggtagtt
    10501 taatcatacc ggcatctata agcatggtgc caattcccag ttcacgaagt ttcagaggtg
    10561 tatatttcaa tgccatgcca ataagcagtg agtaaaatgt gacgttaacg gaatgaacaa
    10621 ctaaataatt ttttccttct tccagtccat ataaaaataa aaagatattg ggataattct
    10681 tcataatttc cattattttt gttaaggaaa catcaatttc atcaatagca aactgttgat
    10741 ctgcttttat ggcgttgtgg acatttccaa cagctttaca tgcatcttta tgtatttcta
    10801 tcaggggcac ccgttgggta agaagcttat tgtaatcatc aatgattttt ttagcttcat
    10861 cgctttcggt catggtagcc cgggcagtag ctgccttgat ttcctgctcg gttgacacta
    10921 aattgccagc agtttcaacc tcactgaccc cccaccgcat aagttttttt atatcatcaa
    10981 gcttgatgga cacattagca gcaaccagca tattgttact gtctatataa acgggtttat
    11041 caaacctcat cccgggtttt aattcatcaa ctggtatttt tcgcatttca gccataggtg
    11101 tcaaattttt atgttatccc cttattcaca tattgtttat gaacttaata ttataaaaag
    11161 tgtattattt caattcaatt tttttctaaa atcactgttt atttgatagc aatacgccag
    11221 tacaactgca acagccctgt aaaggctttc aggtatatat gaaccggttt ccagggatga
    11281 aagtaccgct acaagatctt tgtcctgata aataggtata ttatactctt ttgctttgtc
    11341 aagaatactt tttgcaagtt ttcctttacc cttagcaatg actttgggga tgtgttcttc
    11401 aactgagtac tgtagtgcta cagctatgtt ttccattatg gactttccta tacagaaaaa
    11461 tcaatactat attgtatata catattgtta attaatgcaa taattttttc ttcccaatca
    11521 cttctttgta tacagctaca ggctgctttt ttcccggttt gttcatggat actgcgtgct
    11581 attatagggc attgctgatg taattggctg aaggcagcgt cagtaaaaca gtatatggta
    11641 gcagctatcg tatccttccc ggttatcata atattcaact tccctaaatg tgaaaatgtc
    11701 acatcaatac acacataatt atcatcggaa agaatatatg catcactgaa tgaattgcca
    11761 tcatacaatg gcactgtgaa cacatttccg gagctatcgc ccagatattg tacaaggctt
    11821 tcaatgtcat cttttgacat cattgacata aggctttcaa tatcatcatc atgaagctca
    11881 aaatcctgta atgaactatc gccaatattt tgtaatatat gcatagttaa acttatacca
    11941 gtgtctttag ataaagtgta acaaaatttt tgcagcagcc tttccggtac acctttttgc
    12001 aacaaccgtt gtaaaagcgg ggcaatcacc gtaaatgtat attcccggtt agcatttgtg
    12061 tcaaacagtg ccttgtgtat ggaaaacaca gtcagcgaat ctatagcatt gaggtttcgc
    12121 ataaataatg catagtttga gggtgttgca ctcagtatat tggtattacc cagagcttta
    12181 ggttcaacca gtgaaaaaat aaaacggtta ccagttattg tatcaaattt tagcaatata
    12241 tggttggatt gtggaagccc tctggcaaaa cgtgtttcaa aaatctgatt tcccacacgc
    12301 accatagcgg tgtttgctgt taaacgtttt gtgatggtac ctacaacaat ctgaccgctt
    12361 ttcagtgtaa tggctgatga gtgcgtacgg tgtgcgctaa caatgttgtc tgtaattttc
    12421 atggaaatag cgatagttca tagaataaac gtgcaaccgg ttcataggtc ttccggtgca
    12481 gaggcgatgg tccgtatttt tttatggcat caatatgctt tgcagttgca taccctttat
    12541 ggtgtgcaaa gccatattca ggatatgttt cattaatgct aatcatatat tcatcacggg
    12601 ttaccttggc aatgattgat gctgatgcaa tggaaatcga caggcaatca cccttgataa
    12661 tggacttata cggcagcgat agtggaaaag aaaaattgcc atcaagaagc actgtgtggg
    12721 gtgtgtgtgg gcattgttgc aaaagcgtag tgatggcaaa atatgtggct ccgtttatat
    12781 tcttttcatc aattatttca ggcggcacaa gaaggcaatc ataccacaaa caatagtgtg
    12841 taatgatatc ttttgcctga atgcgttttt tatagggcag cttttttgag tcatttatat
    12901 aacgtaaaat ttcttctggg ggattgctga atatttcagc aggatagata accatagaaa
    12961 cagaaagagg ccctgctaaa gagccccttc ctgcttcatc tatgccagca ataatgttat
    13021 agccttttgt acattcttcc tgttctattg caaaatcagg ttttgcaccc atacagtgtt
    13081 atgcatttgt ttcagtgttt tctgaattgt caacaacctc atgtgttgca ggtgtttcaa
    13141 tggctttttt acgggaacgc acttctttaa gttttgctga tttaccagtt cgttcacgca
    13201 gaaaataaag ttttgctctt cgttttttgc cctttctgac cacagtaatt ttttcaattc
    13261 gtggagaatg cagcggaaat atcctttcaa cgccaacatc ataggaaatt cgtctaactg
    13321 tgaaggtttt gctaatgcct ttattatcaa tagcaataac gatgccttca tatacctgga
    13381 ttcgctcttt gtccccttca attatcctgt agtggacttt aacggtgtcg cccacctcaa
    13441 aatttacgtc acggtaactt ttactgaatt cattttgtac taactgtatt gctttcattg
    13501 ttctacctcg tccttttttg ctttatacaa tctatacaga tcaggcctta ctgcctttgt
    13561 cttttcaata cttttttcca ggcgccattg ccgtattttt tcatgattgc cacttatcag
    13621 tatatccggt actgccatcc cgtcaatttc acgcgggcgt gtatactgcg gatattctaa
    13681 aagataattt tcaaagcttt cctcaagcag cgactgtgga tttgacataa atcctggtat
    13741 atatcggcac aatgcatcaa taatggttaa tgttgcatac tcgccgcctg aaaggacata
    13801 gtcgccaaca gaaatttcat aatcaacaaa acggtcaatc actcgctggt ctatgccttc
    13861 atactggcca catataaaac agtaactatc gtacaaaaaa agctttttta caagttcctg
    13921 tgttagcaat tctcctgatg gtgacgggta aatgactgtt gagtcaccct tatgcaattc
    13981 aatggcttta aataagggtc ctggcataag caccataccg cttccgccac catacggata
    14041 atcatcacat tgacgatact ggccttcgcc ccactgtcgc aggtttatta tttcaaaacg
    14101 gcatagacct ttttcaactg cttttcccat aagcccggtt tgcagggggc tttcaaaaaa
    14161 ttccggaaac agcgtaataa ttttaaagat cttcactcaa taagcccttc aatagggaaa
    14221 atctcaagaa ttcgttcgtg caatcgattg gtgcttacca tactttcaac aaacggtata
    14281 aggtactctt tgccttcggg tgttttgagt accagtgtat cggctgcacc ggtttgtata
    14341 atatcggtta catggccaaa gattgtatta ttatagtaaa cagtgcaacc aatgattgct
    14401 gaataatgat aactatcagg ttcaagtgtt acttcagtat cacctgaatc aatgaaaatt
    14461 tcacatccct ttattgcgtt tgcagcagtt acatcatgta tgccatctaa atacatgtat
    14521 cctgtacgtt ttgctgcaaa ggtaaaacct gttaaggtaa atgctttgta atgcccgcgt
    14581 atgttcacat acaccggaac atcaccggaa aatcgctcag ggaaatcggt aattatatga
    14641 actttaaggc atccatctaa tccatggatg ccggagatga ctccaatacg aaaatattct
    14701 ttactgctca atcaagtatt tccagaacta tccttttacc tgtttttgtg gctgatgcat
    14761 ttaatatggt tcgaattgca cgcgcaatcc ttccatgttt gccaataact tttccaatgt
    14821 cctctttggt gacctttaat tcaagaatag tagatttttc tccttctatc tcccttattt
    14881 caactttatc gggatgatca acgagagatt taaccatgta ctccacaagt tccttgtaat
    14941 tcatcgttat cgcctccgcc tgtaagattg gaataagcct ttatataccg gctttcttaa
    15001 gtaattttgc gatagtttct gtgggctgag cacctttttt cagccactct gctacttttt
    15061 ccctgtcaac aacaaactgt tcacctttaa ctatgggttg ataatgtccc aggctttcaa
    15121 taactgcacc atcacgacgt tttcgtgaat caaccaccac taccttatag aatggttttt
    15181 tcttggttcc agcgcgttgc aatcggatct ttaccacgta tctacctcct gaaataatta
    15241 gtggatgatt atttttgatt agtatttttt gtcaatacaa aatataatac aacatatacc
    15301 ataaatcaag gataaactga aagattttac agagtaatat aaaaagacca tgtattgaaa
    15361 tatatagtag tgtattagca catttgtaaa gcacgtacaa attttattgg gtttattatt
    15421 atcccttttt tgctatttcc agtaactttc gcgcctgttt atttatatca gaagatttaa
    15481 agacactgct accggcaaca ataacgttgg caccagcttt tactacatca gcaatgttat
    15541 cttcgttaat accaccatca acttcaataa gtgtattaca attattggtg cgtataaatg
    15601 tattgagttt ttctatcttt tttaaagaag atggaataaa tgtctgtcca taaaatcccg
    15661 gctcaactga catgagcagg accatatcag tatatgcaag aatatcaaaa agctgttcaa
    15721 caggtgtggc aggatttatg cttatacctg cttttatacc acaatttttt atgctctgca
    15781 acagccgcac cggttgccgt gtgctttcaa aatgtatggt taaaaaatat ggcttgcagt
    15841 ctataaagct ttgaagcgat agctctggtt tttcaattat caggtgtatg tcaaggggta
    15901 tatcagtgtg ttgcttaaga ttttgtatgt agccggggcc aatggtgatg ttgggcacaa
    15961 agctgccatc cataacatca atatgcagaa aatgcatgca ggagtggtca aatgttggaa
    16021 ccacagatcc taaggttgaa aggtcagtgg caataataga cggcgatagt aaaatggtat
    16081 cagtcaaatt cttcaacatc aaaacttaag accttaacct gctttttatt gcacataacg
    16141 tatacacgtg cattccccgt acggtggaac acacaatcaa tagtgttgcc gggtttcaaa
    16201 ttttgcgaaa aacacaacct ctttgatgta ttatcactta catatatttc atacaatcct
    16261 tcgttttcat ctccaggtac ggtgtatttg aggcgttcat acgcataata gggtttgtcc
    16321 ttcatttcgt aatagtatac ctgaatggtt actgtttgcc cttctgtgat tggctggcct
    16381 tccagtgggg taacactggc tataatgcca ctttgtgcaa tatcgctggt aggcagtacg
    16441 gtaaatgaaa caaacacctt ctttgacata agaagaggga aacacaggtc aactgactga
    16501 cccacaactt ttggcataac aggctgtgtg gcgcttccac ttgatacaag caggttaacc
    16561 ttgcggtcca gtacaatctt ttcacctgat tttgggctct gatcaataac cacattatca
    16621 gcagtttttt cagatggtat ataagaaatg actccaacac gcagcgatat agttttatcg
    16681 tacagatgaa gatttttaag cttattgaga gcaaaaggca attccatacc aaccacgctg
    16741 ggcatatcaa tggtgaggtt acttctgctg actaaaaggc gtatggtgct gttctcagaa
    16801 actatctccc ctggttcagg atactgccgt aaaataatgt cattgtcaat atcatgtaca
    16861 tcataaaatt taagctgagg ctttaatcct ttgcggatga ggctgttatg gacatcagcg
    16921 taacgcttgc caacaacatc agggattttg acttcttttt cgggtttggt taataccaca
    16981 acaatgatag ctgaaactat caggtatagt gatatagcta taaaaattat aaacgcaatg
    17041 cggctgtaag ggtgcttaag ccatccttta gagcgttcta taacatcttt cagtgaatac
    17101 ctgggcattg ttttttcctt ctataggtag tgaagtaaac tgtatagtag tatcataaca
    17161 atgagatttg aacacaagca aaatttgttt tttgcgtcaa gaataatttt ggtgattttt
    17221 ttatgatgat agcaaaatgt aatacacatt atacgtgtaa ctattaaata caataatttt
    17281 tatctcttcc agaagtgagg catacaaatt accagaacag tataaagttc taaacgccca
    17341 actatcatgg caattgacag tacccattta atatagcttt cataaaatgc atagtttttt
    17401 gtaggaccaa cttttgcaaa acctgggcca atattaccta atgtagccag ggctgttgtg
    17461 aatgatgtgg taatatcatg cccatcaaaa gctataacaa atgtaacagt aagaagaatc
    17521 attatataaa ggaaaaagaa gcccatcact gcataaacaa tatttttttt caccatattg
    17581 ccactgatct ttaatgcaaa cacaccccgg ggatggatta ataatttcat ttcattgagt
    17641 gcctgtttta aaagtataac aagccgtacc acttttatgc caccacccgt agagccagaa
    17701 cacccgccta caaacataag aataaaaaga attgtatgag caaagggaac ccattgctca
    17761 tagtcagctg tagcaaatcc ggttgttgtc aggatacttg cagcctgaaa ggatgcaaac
    17821 cgtacacatt ctcctatggt ttgataggta gtaccataca atgaaagggc aattaacgtg
    17881 gttgaagcaa tgaatataaa taaaaaagct tttaattctg tatcgcgaat aagtggtttg
    17941 aatctccctg tcaataaacg atagtgcagt gaaaagttta ttcccgctat aagcatgaaa
    18001 acggtaatga caccatcaat ataggctgaa ttgtaatgcc ccacacttgc attttttgtt
    18061 gaaaaccccc ctgttgcaag tgtaccaaat gtatgagaaa ggctatcaaa taaatccatt
    18121 cccccaatca taagcaacag agtttcaagt atagtaaatc caatatataa taaccacagt
    18181 gtttttgcag ttttggtaat acggggtgaa atcctgtcaa ccgttggtcc gggagcttca
    18241 gcgcgaataa gctgtagccc tccaataccc atcatgggaa gtattgctac agccaaaact
    18301 acaataccca taccaccaag ccagtgtgtt aacgagcgcc agaaaagtaa cgatctgggc
    18361 aaggattcaa tatcggttag aattgaagca cctgttgtcg taaaacccga aacagtttca
    18421 aaaaatgcat cagtatacga tggtattgct ccggatatat aaaatggcaa agcgccaata
    18481 attgaagcag atatccagct aaacgtaaca agaagaaacc catcacgggt ggtaagtatt
    18541 tcaacttttt tatgacgtaa aagtatgagt atcgttgccg caataagtat cactactgat
    18601 atagtaaagg caaaagcatg aaagctatgg aattcaccat gatacagtgc aatgccagct
    18661 ggtattgtca taaatgatga tacaattata ataagaaatg atagaatttt gataattaat
    18721 agaggattca cttgctgttt acctcgttaa ccctgtataa attgttcaag gtcaggtatg
    18781 gacgtttttt ctgcaattat gataacaaga tcgttaggct ctattactat gtttccatga
    18841 ggtaaaatgt tcgtgttatt tcgtacaaca gaaagaataa tggaattttt tgggaatagc
    18901 atctctttga gtggtttgcc aactaaagga ttcttttcat caactgaaaa ttcaataact
    18961 tcggctcgtc catgaaagat gctatgcaaa ctttttatgt tacctcgtct gataaatttc
    19021 attattgcat caacggtgct gagtttggga ttaactgtta cgtcaatatc taatcgtgat
    19081 gcaatagcca aatagcttga atttgtgaca agagcaattg accgtttgat ccccatactt
    19141 tttgcataaa ggctgataag catattgagt tcctgattat cggttacggt aactataagg
    19201 tcatgttttg ctatttcttc ttcatggaaa acactttcat cagaaatatc agcattcagg
    19261 ataagcgcat caggaaattt atcagctaaa aacttgcatc gttcataatc agtttcaaca
    19321 acagttattt ttcttccagt tctaattaaa tattcgcagg caagggatcc aacacggcct
    19381 ccgccaatga ttactatgcg gtcaatcttt tcctgttttt tccccacctg aataaaaatc
    19441 cttgtaaaat ttttttgcgt tgccattaaa tagatattat caccttcgcg gacaatggta
    19501 tcacctgttg ggattaaaaa gtcattatta cgcattattc ctgaaataag aaatggttca
    19561 gaaattccct tgcgtatgtc cttaactgtt ttattggcaa aatatgaccc tctggttact
    19621 actatgttgc gcatctgtag gtcggtattt tcaaagaaca tcacatcact gtctgcacca
    19681 agtgcaatag tgtttgctat ctggcgagct gtttctacct ccgaattgac aatcaggtca
    19741 atacccaaga aagatttccc cataatcttt gatttagaat agtcaatatt acggacacgg
    19801 gcaattttta gcgggacatt aaattcactg gcaacaaggc cacaggcaat catgtttact
    19861 tcatcagagt tggttacact tataaaaatt gaagcgtctt tagtccctgc acgttttaaa
    19921 gaagatacat tagtgccatc atcatttatt accatacagt caagatgttc agatataaat
    19981 ttggcgcgtt caggattctt ttcaaggatc actacgtctt tcccttcggc aataagctgt
    20041 tcagctatct gatatccaac gacacctgcg ccaaggatta cggtatgcat tgtttatcac
    20101 cctttatgtt ggatgtgata ctggataaga gaagtataca gtataattca agataaaatc
    20161 aatacaatac attatactgt caataataaa aattgataca ttaaagtaca tttgtaaaat
    20221 taaaataaca tcatattgta tagcttaagc gatagttact gttatttaaa aaaacgcagg
    20281 aatacattaa ccaatagagt tagaatgatg cttatgataa gcatagtcat aaaaggaaaa
    20341 taaacggtca tattgccttt actgtacatt acatcaccag gcaactttcc caaaaattta
    20401 ggaaatacct gaaataagat tccggttaag attaacagta tccctgctaa aattaataat
    20461 ctacccattt tttgttccac ctcctatcaa aagagcgttc atgatgagta tgatcgtgtt
    20521 gttgtcttta gattaccacg cctctgcggg gcttgtaata acggcgctat ataatatacc
    20581 aaaatacttt attcttgaca atcccttcca tatactatag gataaagatg aaaaaatgtt
    20641 actgatgttt ttaagaaatt attatataac aataactgaa tatattcttt gccatactaa
    20701 acttaatgag taggtagtac ttgctatgta tatatgagtt gccattatta gaaataaata
    20761 atggcaaaag attatagtgt aatcatatgg aacattctat tgacaaaaat ttcaggatta
    20821 aagagcttat tgataatctt ccagatattg ttatttcggt tgattatcct tctggtatta
    20881 tacagtatgc aaacgttaat gcaattgaac gtttgggatt ttcaataaaa agagcaaaaa
    20941 cgttacgcga cctttcgtat tttgttatcc caaaatacag aagaaaatat ctacaacact
    21001 ggcgggatat aaaagatggg attctaatac catcgcttgt ttttgaggta aaaacaattg
    21061 atggcagaat tatatgggtt gaacagaaaa atatttatat caaacagggt gattcggtta
    21121 tccggataca tgcagtaatt cgcgatatta ccgagcgcaa acgcttagaa gaagagatgc
    21181 tacaggcttt ttacttttct gaaaatcttt ttttgcattc accatatata atattgctct
    21241 atgacaacga gggatatatc aagcggcaga atatgacatt cagagctatc gctgagcatg
    21301 tagataatat gatacccaat tataatgtgt ggaaagataa acaaatacaa aaatacggta
    21361 ttgatcttat tttcaaagaa gtaatgcgcg gtcgtattat ggatatacct cgattccctt
    21421 atacagttaa aattaaaggt gaggatgtca cattttattt tgctggaaaa gcttttccgt
    21481 acatgcgttc ttcacgtgaa cctgaagggt ttgtggtgat gcttgaagat gtttcggcac
    21541 ggcaaaaacg tgaagaggaa acccttgaaa caaaacggct tgaaagtgta gggcggcttg
    21601 cccgtggaat tgcgcatgaa tttaataata tactggctgg catatcaggt tttgccgaga
    21661 ttcttttacg aaaattgtcc cccgatgatc ctaataggca ttttgcaacc aggatatttg
    21721 aagccgcaag taaagctgca acacttacca atcaattgtt aggatttgcc cgtgggcagc
    21781 tgtataatcc atcgtatctg gatattcgag ctcccataat gtatgccata ggtgccacgc
    21841 caaatctgca taatagaatc actattaaga aagaatttga agaatctgag tataaagtat
    21901 atggtgatgt taaccagctg cggcaggtat ttattgaaat actgcgcaat gctgcagagg
    21961 caattggcac agaaggcgaa attgaaatta aagtaacgta tgtgagtaat gaatttctac
    22021 cagcagagat cagaaaaaaa tatatcaaat gtgttcaggt tgatattatt gattctggaa
    22081 caggaattga tgattcaatt attcccaaag tgtttgaacc ctattttacc acaaaagata
    22141 agttaaagca taccggcatg gggctgtcga ttgtttttgg gcatataaaa aatcatgaag
    22201 ggcatatcac cctatcgcgg gcagaaaaag gtacaaaagt tacaatttta ttacctgcaa
    22261 tcaaagagcc tgaccttgca atattaaagc aaccacgtta tcaacgaata ctggttgttg
    22321 atgataataa tgaaacatgt cttttattac gctcctattt tgaggatagt ggcgcgttag
    22381 ttaccatatc caataatgga ttaagtgctg ttgaaatgat gcagaatgca cccaaccgat
    22441 ttgattgcat atttctggat ctaattttgc ctgatataaa tggtaaggat gtgttaaata
    22501 gaataaggaa ctttaatgct catgtaccgg tatttataat gagtgggtat gacatgtctt
    22561 catttataag tgaacatggt agtgatcaaa atcttttctt tttaatgaag ccatttaact
    22621 tagaagatct ggatgtttta ttaaagtaaa ttttaatgta ttccatattt tttcagtttt
    22681 tcatatagta cacttctgct gattcccagc atcttggctg cacgtgattt gttatgatga
    22741 gccagggata gtacatagat aatatgtttt ttttcaaatt cttctatagc tctgaaaagg
    22801 ttaaggggcg atagttcctg tgaaattcct tccttatatt caggtaaaat ttgcataaat
    22861 gtttcttttg acagtatggt attgtcctca acaatcattg catgttcaat aacattctgt
    22921 agttcacgga cgttgccttt ccattctcta ttggaaagca gttccaatgc atcatcacgt
    22981 atgccgataa tggttttttg gaggtcatgg gagtacatat gtataaagtg ctcagcaagc
    23041 actggtatgt catcacggcg ctcacgtaat ggtggtatgt gaagttcaat tgctgagaga
    23101 cggtagtata ggtcttctct gaaattcccc tgtgaaattt cttcatccac gtttatgttt
    23161 gttgcgctta taatccgtac atcaactttt actattttgc ttgatcctac cggtttgatt
    23221 tcacgctcct gtaaaaaacg taagagcttg ctctgtatat gcggttgaag gttcccaatt
    23281 tcatcaagaa atatagtacc accgttggct tcttcaaata gtcctttttt atttttatct
    23341 gcgccggtaa atgctccttt aatatggcca aatagttcag attctatgat ggtttcagta
    23401 agtgtgctgc agtcaacagg tataaactga ttatgttttc tctggctttc ttcatgaatg
    23461 gcacgggcaa taagctcttt gccggtaccg ctttcgccca atatcatgac agtagctttt
    23521 gagccagcta tccgctttat tttgttttta agagaaatga tagttttatt gttgccaact
    23581 attctatcaa tacctgttct ttcctgtggt aaagtggtta taatttccat tacagttggt
    23641 gttgaattaa tcccttgcat tttcccttgc tgtaagattt ttatattgtt tctgtggcaa
    23701 aaatacacca ctgttacagt atgcttattg ctttattact atgctttcag ttttaatttt
    23761 ttatattact ttcaatgccc cttaaataaa agggctacat catttttacc agtaatcaca
    23821 ataaatagta tttatacaat cattttttta aattatgttt gaccaaagtg tcgttatttg
    23881 caactataat tcataatttt tttaaaaaat tatgtaacgt atactccgct gcttgttgcg
    23941 gtggtatgca ggtatttatt aataaaatat tctgaaggaa taattactta aaaaataatt
    24001 acatgcgtag aaagtgaaat ataaacaatg atttcgattt tgttgatgtc tgacatccat
    24061 tttggttcag ataaggcact gccatatatt gatgacagtg tcagatttgc cacgttccag
    24121 aagatctgta tgattgccag gcagcattcg atttttatta ttgcaggtga tttatttgaa
    24181 ggaactatcg cacagaatcc ctattatagt tccattaaag aactgttgca gtcattagta
    24241 caggaaggcg taaaaattat atacattcct ggcgaagctg aactggagga taacaacaat
    24301 atagatgtta tacatgaaat tccttctacg gttacgttat ttggccgctc caaaaattat
    24361 tttgatcttg ttcttgataa tgagattgtg tattgctatg gaggggatag tgacatcgcg
    24421 agtattacaa aggcaagcca ggaagggttc catattggta tttttcatat tgaccttgaa
    24481 gaaaataatc acaataccgt tgattcccat gtgatttcag ttaaactgtt aaaatcgatg
    24541 ccattagact tttatgcact gggacatcac cataattttt caatgtataa gtataaaaat
    24601 aaaattattg gagcatatcc cgggagcccg gaagctgtag cacctggtga aaccggtgac
    24661 agatatgtcc tttcaattac tgttatgaat aatgaaattc atcagataaa acgtataaca
    24721 gtaaattcat tacggataga gtcgctgact atacagtgca atagtattaa gtcaaatgat
    24781 gaattgcatc atatacttaa gccatataac aaaccttcca ttgttttaca ctgcacactg
    24841 gaaggagtcc gttcatttga cagtaatcat attgtggctt tatgtgacag gtgtgctggt
    24901 gtaatattcc aggacaattc aaagctttca ggtgaattta tatgtgctaa gtataaagat
    24961 gagaaaacac tcaaagggta tttttttact gaattgcaga aaaattttga caatgttgct
    25021 ggggatataa tgctggatat tatcaatgat attgaaaata cctgttcaac atataaaggt
    25081 gatctgtgaa gttagtacgg agtatagtat caaaaattgg atttgcagaa agcaaacaaa
    25141 cagattttaa tgaggggatt cattttatct ttggcaaaaa taattccggc aaaacactca
    25201 tttcaaaaag ctttattgat acaatctatc ctcaaaatcc atcattaatt gatagcgaag
    25261 catgggatac aatgtttgcc acatttacgc ttgaatgtag tcaaacaaaa gttgagattc
    25321 aaagaaaagg caataaagaa ataaaaatat ttgaaattac atcaaatggc aatactcaaa
    25381 acgaagatac ataccctttg agtttcaatc cggggagttt gtccgttaac tgtctttcta
    25441 agttgcacgg actttttaat aaagatacat ttgcaatcct ttgttatatc ccttcaccgc
    25501 ttgatacaca atactacagc aaagattttt ctgacagcat gaaatcagtt cttttgttag
    25561 atagatcatt atattattat gccttttcca ggttgtttac tgatacgata aacaaaacag
    25621 gcaattatca ggctcatgtg ctggcagaga ttcagaacaa aataaaacat tgcaaaacaa
    25681 aacttgattt actgcaactt aaggaacaga aacagaaaaa acatcgtgca gaaatacagg
    25741 aagttaatga aagaattacg tcatatacca atgacattaa tgaacttcgg aaaaagatag
    25801 aagcctgtaa tagatatatg gatacactgg ttgctattga taatgttaaa aagcagatag
    25861 cggatgttaa tggtcagata gaagctcttg aagaaaaatt agaaactata gaaaaatata
    25921 caaaactgat agccagcaaa tatccccagt ttaaagactt ttcctcggaa aagaggaaca
    25981 atattcatgc aatgcaaaac atttaccggc agatacaggc tatttctgaa gagatagaaa
    26041 ctattatctt tggacaggaa agcaaaaatc atgcacttgc acgtaacctg ctcattatta
    26101 atattatact tgcgttaggg gtgctatttg tatttttaaa accagttttt gggataacct
    26161 ctatgcatcg ctggctactg gtaagtttat taattttgat tcagatatca agtatagggt
    26221 attaccttat ttatgtaatg atgcattcgt ttaaaaaaat agtcttgccg ttacagcaac
    26281 aaaaagaaac atatcagatt aaattagaga cattgctgaa tcagaatggc atatcactgg
    26341 atgggataac catcgatgaa atatatgaat ttctgttgca gtattttgaa gaatataatg
    26401 aatataatga aaagcagata gaccttttga cgatacagaa agaaattgat gagagtactt
    26461 caattgatat tcttgcagag gaaaaaagaa agcttgaaag caatgtaaag atgttgagtg
    26521 acgagcttca taacctgcaa gaagatattg gaatagcaat agaatctgca gagcattgta
    26581 tgacaataaa aatgagtttg cacaatgaac tggatgaaaa aaaagcttta ctggttgaat
    26641 cacagaacaa gctggataat atacctgagg agattattga ttatgaagat gagaagaata
    26701 gtatattaca ggagatagat gatctggaaa aagagctgaa aactataaat gaaaaagatc
    26761 gtctcattaa tattattata acttcatttg atactgcaat gcagcgttat ttcaaggata
    26821 gatttaatat attaacagga aaaataaaaa taatgctaca ggaccttaca caaaataaat
    26881 ttggaaaaat tgatgaggaa tatataaaga aaataataac acattcaatt aatacgccca
    26941 acccctcatt aaaatactgt atatatgttg ctgcaatctt tgctctgtat gaattactcc
    27001 agcaggaatt accaaacatt catctgccat taattattga tgatccattt gtgcttatgg
    27061 atgaagaaac aatgggtgag tttatcaaaa tattacaact gtgggtcggc aacaggcagg
    27121 tgattatttt cactcataat tccaatataa aaaaattgtg tgcagttact gaattgtaat
    27181 tatggataaa aatggacagt taacattttt tgatgatcct ttgcttcagg gtattaatga
    27241 agcgtataca tttattgaag caggtgattt cactcaggca gtaaaaaaga tagatgacct
    27301 tatgaccata aatcccgatt acccgggact ggcagaagca tacagggttg cgcgcttctg
    27361 ggaatcaagg aaaaaggcaa tgcaggaact tcaaaaaggg aaggacacag cagattttct
    27421 tatgacccag tgggaggagt ttgaagcttt tgcgcaaaac agggggcttg tttgggcaac
    27481 agcatatcaa aaagcaatgc ggtttgtttt tttcactgct tctgagcatt acactattgc
    27541 attcaatgac agggaaaacc caactgataa tctgcagctt cttatgcaac tgggaacttg
    27601 ttttattagg ttgggcgagt atcagcatac tattgaagca ttagagtatg ctcgcagtgc
    27661 ctaccgaagc aacgcatgta tattatcact tttagctgaa agctattatc atactggaga
    27721 tgtgccgcgg gcattacttt atttcaggga ggcgttcctg cttaatccgt ctgagattga
    27781 tatccagtta ctatcggcca aacctattat tgaagcgtta gaactggtta agcaacataa
    27841 acctgattgc tctgatgtgc gggaatgggt tccggttatg gcacatattc atgatatttt
    27901 ttatgttaag cgacagctca attcatcaca gcttgaaacc ataaaaaaag atattgtgaa
    27961 attagaggca agttttcaaa cgctggataa agagaaaata gcacaatcaa atattgtgcc
    28021 acgccttata aatcgctatt tatggatgct tgattatttt gaatttcagc aatacgatca
    28081 aaaaagtctt tttgatatcc acgaccgact gcttgccatt gataaaggtc tttttgaaag
    28141 ctactttcaa aaaaagactt tttcaaaaca ctgatatttt ttaagtgcaa ataatgaaga
    28201 attccatagc tttcagggga attcattctt cgtaaaaaat tcattgacac tatatatcgt
    28261 actcaataat taacatagct attattgttt cacggaatat tttttgacaa taggtggtag
    28321 aatatggaat tgtcagcatc atacgatcca agaattgccg aatcaaaatg gtatgcaatc
    28381 tgggaaaagg aagggctttt tcatggtgat gaaaatgacc cgcgtgaggc atattcaatt
    28441 gttataccac cacctaatgt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28561 atgttaggtg ataccgcggt tgccgtaaat cccaatgaca gtcgttatgc ccatttaata
    28621 ggcaaacggg tgatattgcc gctggttaat aaacctatac ctattattgc cgatgagttt
    28681 gttgaccctt cttttggtac aggacttgtt aaggtaacac ccgcacatga ccccaatgac
    28741 tttgaaatgg ggaagcgcca taatcttgaa gaagtgaata tctttgataa aaatgcaatc
    28801 gttaatgaaa acggtggtgt atacaaaggc cttgaccgct atcaggcccg tgaaaaggtt
    28861 cttgaagatt taaaagcgtt gggcctattg gaaaagatag aagaccataa ccatgcagtt
    28921 ggccattgct atcgctgtca tactgttatt gaaccgtatt tgtcaaaaca gtggtttgta
    28981 aagattcaac ctctggcaca ggaagcaatc agagttgttg aagaaggcaa aatacgattt
    29041 gtaccccagc agtggacaaa gatatacttt gaatggatgt ataacattaa agactggtgt
    29101 atttcacggc aattgtggtg gggacaccgc atacctgcat tttactgcaa ggattgcggt
    29161 cacatcatgg tagagcttga tgacccaacc gtgtgcagta tgtgtaattc aaaaaatttg
    29221 gaacaggacc ccgatgtact tgatacatgg ttttcgtcgg cattatggcc attctctacc
    29281 tttggctggc cacaaaagac aaagtcgctg gaaaaatttt atcccaccag tgtactggta
    29341 accggttttg atattatttt cttctgggtt gcccgcatga tcatgatggg ctgcaaattc
    29401 atgaatgatg taccatttta tgatgtatat atccatgcac tggtcaggga cgagcatggt
    29461 cagaagatga gcaagtcccg tggcaatgtt atagatcccc tcatcatgat ggataaatat
    29521 ggcaccgatg cattcaggtt taccttagct atatttgccg cgcagggcag ggatataatt
    29581 ctatctgaaa agcgtattga aggctacagc gcattctgta ataagatatg gaatagcacc
    29641 cgttttattc tgatgaattt aggcaatgat ttcaaggctg aagccattga catcaattca
    29701 ctggacatgt ttgataagtg gatactgcat gcattcaatc agaccgtgaa gaagacaaca
    29761 caggcgcttg tagagtatcg attcaatgat gcagcacaaa cgttgtatga tttctggtgg
    29821 catgaattct gcgactggta tattgagctg tcaaagccgc gtatttatgc gggggactct
    29881 tcaacaggaa ctatcgccaa acaggtactg tattttgtgc tgcatgaata ccttaaattg
    29941 ctgcatccgt ttatgccgtt tattaccgaa gagatatgga gtaatattac aagcggagaa
    30001 gggcgcattg tggtggcacc atggcctcag tatgatgaac gttttaattt tgaacatgag
    30061 tttaataatg ctgaattgtt taaggagata gtatataaaa tccgaaatat ccgcggtgaa
    30121 atgaatgtac cacctgataa aaaggctcat gtagttataa aggccggaaa aaaaattgaa
    30181 cagtttatac aacagcagga gcataacctg aaaattttag caaagcttga agcagttgaa
    30241 tgcaatgctg actacacacc gcagaaaaca gatgcctcag cggtgcttcc ggaatgtgag
    30301 ctttatatgc cacttaagga tttaattgat cttgataagg aaaaagcacg ccttgacaaa
    30361 gaaattgcac gtatacaggg tgagcttgat aaagtaatga aaaaattaaa cgacagcaca
    30421 tttcttgaaa aagcaccagc gcatattatt gaaaaagaac agaataaaaa ggcagaactt
    30481 gaagaggtgc ttgggaagct caccacaggc agggaaaaat tagggtaata taaccagcac
    30541 ctctatcatt gttggtagag gtgcttatac tcatagttag tggaggttca ctatgcatag
    30601 aagaaagagt attgtattaa ttattgcatc actttttgtt tatgtttcct gttcttcttc
    30661 tttactgaac tttttccgat ggtacgaacg ctctaaagct ggcttaactg agaaaacagt
    30721 tatgattgat ggccacacag tccattacct tgagggtggc aaaggtgaca ctgttctttg
    30781 cattcatggt tttggtggca ccatggatca ctggacgcgc tttgcacggt atgtgacgga
    30841 tgcatacaga gttgtggcgc ttgaccttcc aggttttggc gatagttcct ttaaagaagg
    30901 cggtgactat tcaatagcaa aacaggtaga aagagttcac gaatttgcac acacactgaa
    30961 attgcagaag tttcacattg ttgggaactc aatgggtggg tatattgccg gctactatgc
    31021 aattacctat cctgacgatg ttattacatt gggattgttt gcagcagccg gtgtaaaaag
    31081 ccccaatgaa agtgaactgg caaaagagct gaaaaaagga aacaaccctc ttatcgttga
    31141 agatgtggaa agctatgata gactcatgga atttgtgttc tataagccaa tatggctgcc
    31201 agggtttgca aaacagtact ttgccacaca ggcagtggca aagaaaactc attttcagta
    31261 tatttttaag cagattcatg atgttacact gcttgaaaaa tatctgggta atatacatgc
    31321 accaacgtta ctattatggg gtgaaaatga tagggtgctg gatgtatcag gcgcgtatgt
    31381 ctttaaagaa aaaatacgta atgcgcaact ggtagtattt aaagaatgtg gtcatctccc
    31441 aatgattgaa aaacctgaag aatctgcaac tgtatataaa aggtttttaa agggtcaaaa
    31501 ggtggagtta taattttttg taaagcatat cttcataacc ctgaagcatg gcaaacgtag
    31561 cacgatcaaa atacagcacc acacggttgt ttaacgccag tgcttcctta taggcagtgg
    31621 tgtcatcata taatttatcc agtacaaaaa gccacagatg tcgtttctgt aaaattaatg
    31681 ctttacatgc ttgagaaaca ggaattccct gttcaaagcg ctcgtgccca atccttcgat
    31741 agtagcgggc aatttcttct ttgggataat ctctggctat ccactttgat aattcacggt
    31801 acaggttgtc tgcagattca taaatgagct gtcggtctaa cttcctgaat gctatggtat
    31861 caggattacg caaaagattg ttcataaact gttcagtaat aatctgatgg ttttcttcaa
    31921 taagctcaac aaacctgtta atcagtgcac ttttgatttt catagtattg tattatgata
    31981 atatagtcta atataatgat aaaaaatatt acgattatct atatgtaaag tattttttta
    32041 cattacttct tttttttggt tttggctcta taagcctgtg agtagatttt ttcaaattcc
    32101 tgtgcataca attttgctat ttcagatgat ctgatcatga tgcaattttc atcatttctt
    32161 ttcctggcat tttttgaaaa attatatgag ccagtaataa cccattcatc atcaataatg
    32221 attaccttat gatgcatcgt tttaggatta gcatcataga gaacatcaag cccttcaact
    32281 tttaatttaa tgtattcact gttgacattt ttaatatccc gtttttcaaa aacaccttta
    32341 acagcaatac cctgcttgtg tttagcaata attgcttcac ctatagcatc actggtaaat
    32401 gaaaaagcta aaaataaaat ggattttttt gctttttcta tacgctttgt aatgatgcgc
    32461 tcaatatcat catctggtga aaaatacaca ttaatgtcta tatcattgat tttgacataa
    32521 tatttatttg atagaaaagg gaaaggggta tattcagtgc gattgccaaa tatagcctgt
    32581 acaaacattt cgttaaattc tgccaggtat atggatgcta tctcttcatt ttcaatatat
    32641 atggcattat tgttgttgtg ttttgcctcg cgttcagtaa tgttatatga cccggtccac
    32701 acggctttat tgtcaaaaat aaagaattta ttatgcataa atccttttcg ctcatccagt
    32761 cgcagaggta tgttggcttc ttcaagtttt gttttaacct tttcttttaa tctgtctgat
    32821 tccagtacta acctgacatc aataccatta ctcttagcct tacagagagc atcaataatt
    32881 aatggatcag tgatttcaaa aagagcagca tagatagatg ttttggtaga atgtattttc
    32941 tgtaataatg ctttctggat agcagttgtc tgttcttctt tttctggcgt ggtaaagtat
    33001 acttcaatgg gtaactgagc gtaaacaaac gaatatactg taattatcag taaaaataca
    33061 atgggtagtt tggtataatg cttcattgct gtttttccca gatcaagttt tcatattcaa
    33121 caaatcgtaa actggcttct tttgtgggga aaagcaattt agccttttgt attaacgtct
    33181 ggaatgttat caggtcattt ttgctggcct gtgttatgaa ctcctgtatt ttttcatcat
    33241 tgtgtgttat cagatgaaaa taaaattcgt atgttttttt caattctttg agtttttcaa
    33301 catgcttctg aagttcttct ttgtctgaat ttttaatcat tgtagaaaga aatgcaatga
    33361 tatattgtgc atctgattct gtaatgtcca tatcaagtat aatttgtttt acgcggacta
    33421 aatcaagatt acgcagatga attcgtgcca gaaatacagg attttgcgag agcaaattaa
    33481 tatcaacatt attcatctgg tcaatggcac gtttggtatc atcaatagta acagcagaag
    33541 ttgtattaac tatctccaga cgagatgaag gagatataga agatataaat gtaatatcgt
    33601 tgttactgaa tgtgctattg acaattatat aatcagggac attgcccttt tttacatagc
    33661 tgacaaatga agccgtatca ttaaaaaatt ccgtaataca gggtaccata tcaaggaggg
    33721 tatcagaggg gaaaaaaagg tattgtcgtt taccttcacc agcattgtag gaaataatta
    33781 aagcgttatt ttgaattttt aataactgat aggggtgatt tctggttgaa gtgacaggtt
    33841 caagtcctat gaaaacaatt gttttcataa tatggaaagg ttttgtttta ttatcatcgg
    33901 aatagataat ttcccctttt ggattgaagg caatatactg tttttttgca atgtttattt
    33961 ttaaagtgta gccttttggt gttttttcct caactatggg tgttgacata gtatgtaaac
    34021 ctttaaatag taattatgaa aagtggtaaa ttttcattga aattttataa ttagttagca
    34081 cattgtgctt tttacatcaa ttattacgat agttacaatc tatgtataca gaagcaataa
    34141 tttaaagatt aaaatactgt atacccatta aattgtaatt gataaaaaat caagcaaatg
    34201 taaatattta atgcaaaaat atagtatata taatttcttt aatagtaagt ttatattcta
    34261 aggagaacac aatgaataca attaatatac tttctcctgt tcggtatcca ggaaaaattc
    34321 atgccgtatc aagtagtttt gaaacacatg aggccgacaa tgttttacat aatgatgaat
    34381 cattctggtg tactgcaaaa agccataatg ctgtcagtga atttatcatt atagattatg
    34441 gttcgatagt tgctgttaat attgtcgaaa tctatccttc cccttcaggt aagacaacat
    34501 ttccttcaga atttcggtta gaatgtagca tggacggtat taactgggaa gttctcaaag
    34561 cagagaataa atttactctt gatgaagatg catacaggct attattgccc ataacagtat
    34621 ttcagtattt aaaagtgtac ataataaaac caaagcgtat tgcagcaaaa tactttgctg
    34681 aaattggaaa gatacaggca ggtattcagg gcgctgtttc aattactgct agttcaagtt
    34741 cttcgtatga acatgatccc tccaatctta tggaaattga taaccagaaa tactgggaat
    34801 cggagatcac cagtagtgca tcgcgtcagt ggattgaagt ggattttggg atgatatttc
    34861 cgatgaccaa tataggtatt attgcaccac aaaaccctgc acttttcccg gagcagtttt
    34921 ccatagaaat tactgaagat aaaaaaatat ggacaacgct tacagatgta aaacggttta
    34981 aagcccaacc aaatgggtcg tatatgtttg gcggatatca gtttaatgcc cggtatctac
    35041 gcattaacat tatgactgtg caaaccgaaa aacagaaata ttgtgcacaa atagcgggca
    35101 tttttgctca tgcggcgtta ccagctttga ggcattttca tattaatgct gcagtgcagt
    35161 atgcaacagt ttttcaacca ggcattgtaa aactggcagc tgatggagat actacaccag
    35221 gtgctgttgt tcaggcaagt gatccacggt tacaagatgc cagcaccata tttaaaggga
    35281 tagtgcagct ggctgatgat ggatcaaacg aagaagggtt agttgttcag gcatcggatt
    35341 cacggcttgc acccgcaact gaactgaaac ctggtattgt ccggctggcc tatgacaggg
    35401 aagtagccga gggtgcggta gtacaatcaa cagattcaag gataaaggaa gcaacagata
    35461 caacgtttgg tatagttaaa ttatgcccta atggccagta tataaataat gcagttgtgc
    35521 gtggtgatga cagcagatta caaaaagcta caacaaaaga ctatggtata gtgcgtttgg
    35581 ctgaaaacgg tgaggataac gaattctgtg tggtacaggg caatgacagg cgcttgaagg
    35641 atgctaccat tgtttcaaag ggaattgttg aact
//
LOCUS       DAORYL010000021        35134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_985_length_35134_cov_14.823313, whole genome shotgun sequence.
ACCESSION   DAORYL010000021 DAORYL010000000
VERSION     DAORYL010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 35134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35134
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_985_length_35134_cov_14.823313"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..726)
                     /locus_tag="PLV81_05875"
     CDS             complement(52..726)
                     /locus_tag="PLV81_05875"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOM09664.1"
                     /translation="MITNRHEKSFLIYEDKVALLKLRAQLELIVALLGFGYNDIQIIN
                     ANNPELAKYNGKFLKDIAKERGMSQYENFIDFAKKSNGRARVLNHRYSSFQNILDLMK
                     HRASLFMTDATPYSQGAQNPAAFGNYPRFFEIARDYQLLSPQELIRKMTGAIADRFML
                     KKRGYLKEGYMADITIVDWKNVKDNNTLTETNKRPSGIDYVFINGKKVVDKGKATGVL
                     DAGMVV"
     gene            1080..2375
                     /locus_tag="PLV81_05880"
     CDS             1080..2375
                     /locus_tag="PLV81_05880"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOM09665.1"
                     /translation="MAKGDRIAIIDGCRTPFLRSGTDYREMMAYEICRHAVKGLIAKT
                     GIPNDLVDHVIMGGVANDIATTNVAREIMLAAGLPYTIPAYTCTVACISANAAIISGA
                     NLIVNGNAECIVAGGVETFSDPDIKISKNYRRFILDLTMFKRPKGIIGKLKLLKGMSF
                     KDFIVPQQPAIGEFSTKMRMGDTADRLAKRLGISQREQDEFAALSHNRAAAAWERGVL
                     KKEVVPVVTPQGKLVEKDNGFRKDTTPEKLAKLKPAFDRRYGTVTAGNSSFLTDGGAA
                     VLLMSETMAKKMGIKPLAYIKSYAFTAQDLWEELLLGPSFAIPKALAMGKLKFKDIGV
                     WEIHEAFGAQMIGVIKTLASASFCRDRLGLPGKLGEIPIDALNVYGGSLSLGHPFGAT
                     GARLVTTCANRLRETGKRYGVVAGCAAGAVGNAIILERA"
     gene            2389..4512
                     /locus_tag="PLV81_05885"
     CDS             2389..4512
                     /locus_tag="PLV81_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOM09666.1"
                     /translation="MKDLQFLKTEVINDVLVVSINCPGKVNKVSSDLLREIETIINQT
                     KGKPYKGMVILSEKPDNFVVGADIDELKAMKTDEEIKQYISKANDILFKLEKLPFPTV
                     SMINGNCLGGGVELTLVTDYRIATDSTDTVMGLPEIKLGLIPAAGGTQRLPRLIGIQN
                     ALPLILQGSNVRPRRAKRLGLIDEIVIPYGLKEIGVKKALQLKGKRTIKRKRKFVEWL
                     LESNPLGRKIIFSQARKMVMKQTRGLYPAALAAIDSVEYGYKTNVTKGVAKDIERFVT
                     LVKSYQSKSLMNLFFAMTDLKKNPLEKKAKNISKLAVIGTGLMGTGIAQVSLPLCDTV
                     LVKDMALDAVSRGMAQIRKSLEKRARSGAIRDFDKEILWGKIVPCDDYVNFKNTDLVI
                     EAVFEDLQLKQNLVKEVEAVTGDNTIFASNTSAIPISLIARASKRPQNMIGMHYFSPV
                     PAMPLLEIIKTPKTADWVVATALKFGIAQGKTCIVVKDGPGFYTTRILAPYLNEAVFL
                     IEEGVPMNDIDRALLQFGYPIGPVALLDEVGIDVGAHVAIELGQEFKARGAAPSNVLP
                     LLFSKGFKGKKNKKGFYDYSKPKKKGMRVPNEEIYKVIGKVTWQHLPMQEIQERVSLM
                     MINEAALCLQEGIISSPRDGDVGSILGLGFPPFRGGIFRHIDREGVKTIVDTMMKYYD
                     KGMKRFTPAQILVDMAKSGKRFYKE"
     gene            4516..5661
                     /locus_tag="PLV81_05890"
     CDS             4516..5661
                     /locus_tag="PLV81_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOM09667.1"
                     /translation="MKRSIQFSQEHDIFRQTVRKFFETEVMPHYDKWEEEGIVPRQMW
                     KRSGELGLLLPWLDEKYGGPGGDFLYSVIVSEELAYSGANSFMIPLHNDIVAPYIYSF
                     GTEEQKMKYLPGCASGDIILAVAMTEPEAGSDLAAMKTSATLDGDSWVLNGQKTFISN
                     GILADLVVVAAKSSKEAPPHQAVSLFLVENGTPGFTKGKAIKKIGLKAQDTAELFFDN
                     CRIPKENLLGQEGLGFIYLMQKLQQERLVCALASQAAMEKCLAITIDYVKERKLFGKP
                     LSKFQNTQFAIAEMASEIAVGRSFIDDLILHHIRGEDVVKETCMAKYWICETAKRVAD
                     RCLQFFGGYGYCTEYPVSRFYVDARIQTIYAGTSEVMKMIISKMMGL"
     gene            5757..6503
                     /locus_tag="PLV81_05895"
     CDS             5757..6503
                     /locus_tag="PLV81_05895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOM09668.1"
                     /translation="MNPVIRKGYTYMAWLFIGVDTAFWGTISLFSWFVSPRGNLAHFC
                     MQNWSKNILWFCGIRYQVEGLENITKDKVQIFASNHQSFFDVWLLSAIIPVKFGWTAK
                     KELFKIPFMGWHMTLSGYIPIDRKNRERAIESMKQAAIKIRKGNRIMIFPEGTRSRTG
                     VLQPFKKGLFYLCIETGVPIVPIYIHGTYQRLKPESGDINPGKVYVLVGKEMPTEHYT
                     VDTIEQAMEDLRQRILALQNECLQRYGEAV"
     gene            6537..7823
                     /locus_tag="PLV81_05900"
     CDS             6537..7823
                     /locus_tag="PLV81_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HOM09669.1"
                     /translation="MEDLKKAYRTIMDDHFPDTMTITFGNQTLVYRKRTWKINDEGQL
                     IEKGLRYGENPGQEAALYELVNGNLVLGDCAFITPGHSLVSGIDESMMLQFGKHPGKI
                     NFTDIDNALNILKYLMQKPAAVIVKHNNPCGVAYGSTIADAYNRANRADRIAAFGGCV
                     AFNRSVDKETAQLISANYLEVLVAPDFEPGVMEILAKKKDLRIVQIKRIDRLEDYKTM
                     RFVDFKSLIDGGIIVQQSPINIVNTKEDLKPAQTEYKGQLYKIDRLPTEKEYEDLLFG
                     WFVEQGVTSNSVLYVKDGVTVGIGTGEQDRVGVAEIAVQKAYTKYADILCFDKYGISY
                     YQLALEVEKGIRKEADKIEIDEATKEAKGGLIGASMVSDAFFPFRDGVDVGIKQGITA
                     IVQPGGSIRDWESIVACNEANPKVAMVFTGQRAFKH"
     gene            complement(7838..8413)
                     /locus_tag="PLV81_05905"
     CDS             complement(7838..8413)
                     /locus_tag="PLV81_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09670.1"
                     /translation="MNYRVVKQFWVNTVDNFFNFFYKIVDFIKIWADVFWAFLDIWYH
                     FFAIFGNLFLYIYYIFLFIIDRTTESGTNIVLTSRLPKRVPFRPQKAYIKDSYNPIPA
                     MYGVKNVADTVATSITPMRTAPKGTRTSITKTILSGLRDFIELIVDLFKKPVKAIADF
                     AARRMKPVKEEQHKTGSLIEEYMKEYEQKRR"
     gene            8991..10247
                     /locus_tag="PLV81_05910"
     CDS             8991..10247
                     /locus_tag="PLV81_05910"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOM09671.1"
                     /translation="MALIVDIFMALSHSIDLVDHNIGNHNKRVAYIASRIAHAMGLST
                     GDITRVVVAGALHDIGVLKEKEYKELVHFDYKGGIDYHSLMGYRLLSSCTLTKDVAEI
                     VKYHHVYNSEKKNIPENHTVPIESEIIHIADRIDVLLDHSGDLLEQKNNVIATLEQYS
                     GDRFHPDIVNCFIDIANQESFWFDLQFNSIEKKIKNSIYYNPILSLEDIHEISKLFTR
                     IIDFRSRYTATHSTSVAIVARELGALLNLSDRECLMLDIAGHLHDIGKLAIPLSILEK
                     PDTLSPQEWRVMKQHTYFTFHILDEIDSYIFKVINEWASLHHEKLNGKGYPFKFSGKY
                     ITMGSRIMAVADMFTALAESRPYRDSMDDDAIKKEMKKAKGEELDSYIVDVLLNNYSY
                     LKDIRQRAFVTALQGFEDCFDYISKK"
     gene            10342..12159
                     /locus_tag="PLV81_05915"
     CDS             10342..12159
                     /locus_tag="PLV81_05915"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOM09672.1"
                     /translation="MNAIFHKINAWTIKAKVTAGVIVVLIVSLAFTNVFWRIGYIKEV
                     ERQFVDKARAICTMGEAFREYMADNWERQLYDKDYLVKDIKGKFVYSVPVFSSIVAME
                     KKAKDLGYEFRVPKEYPRNPNNIPTPLEQRILNEIKSKNLPEYFMIDYENDVLRYFRP
                     VKLTKDCLICHGDPAQSYKLWGRKDGKDPTGGPMEGWKEGEIHGAFQIIYSLKDYFAA
                     RLQILIISIVVNIIILGVAIVIIRRVVHRGLHPLDEMVQSLEDINKGQGDLTRKIAIE
                     RDDEVGRLALLFNQFIDNLRNLILAVKDAADHVASSSVEMTQSSQQLANVAQDQAASI
                     EETSSAMEEIKATIDSVSENAKGQAKKADATRNSMEYLAEAIERINQNAQDAYKMADE
                     THGYAKEGEAVLGSTVSGMRAINESSRRITEIVTIISDISDQINLLSLNASIEAARAG
                     EHGRGFAVVAEEISKLADQTAQSSKEINKLILETNNKVNAGSELVEKTANSLRKIIEN
                     VKTTAVLMENIAQSSQELNRMSNEVKTDVEEVNKMSEEISIMMEEQSASTNEIIKAIN
                     QINEVTQIVSSGSEELAAASEELSSQAETLNNIVKQFKV"
     gene            12177..13430
                     /locus_tag="PLV81_05920"
     CDS             12177..13430
                     /locus_tag="PLV81_05920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOM09673.1"
                     /translation="MEYSTALRPFIIKRLEQVKKADILVGIPCFNNEATIANVMTMVS
                     DGLFTYYKDLRPVIFVSDGGSTDDTRDIAQSIELKPWQERIIQIYRGVAGKGSAFRAI
                     FEAARILDVDACMVVDSDLRSISPQWVQRLLSPVLEKKYQFVAPVYTRYKYDGTITNN
                     IVYNIIRAVFGKRIRQPIGGDFTFSKRLAQLYLDKPVWSTDIARFGIDIFMTVNAIVS
                     HVEVCQANLGVKIHDAKDPAQSLGPMFVQVIGTLFRLMEHYEPYWKNIEGSKPVPTFG
                     DIDMIEPEAIEVNLQRLVDEFKTGFIQFNALYREMFSNQVFTSLKDSAHMDVESFKMP
                     VEIWVKIVYELAAIYHTWQINKVRLINLMVPLYFGRIASFINETISMNSFEAEEVVEQ
                     QAQVFEHYKPYLMEKWQATGKNIEL"
     gene            complement(13432..14154)
                     /gene="rlmB"
                     /locus_tag="PLV81_05925"
     CDS             complement(13432..14154)
                     /gene="rlmB"
                     /locus_tag="PLV81_05925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOM09674.1"
                     /translation="MDTQIVVGRKVVLEYLTTLQGDATLYVSESAHGKIIDVIIAAAH
                     SKHIPVQKVNKSWFDSHCAAHHQGVALVMNYRPKKEEKQCELKKIASSHGVIVACDEI
                     TDPHNIGSIIRTTEALGGSAVVVTKAHAPEITPTIIKASAGATAHIPVLQVPNLARFL
                     DDAKKAGFWVIGTSDRGTQPINTIATYKPAVVIIGSEGSGMRQLTQSLCDIIVRIPLK
                     GKVSSLNASVACGIVLWELLKE"
     gene            complement(14136..15560)
                     /gene="cysS"
                     /locus_tag="PLV81_05930"
     CDS             complement(14136..15560)
                     /gene="cysS"
                     /locus_tag="PLV81_05930"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOM09675.1"
                     /translation="MALKIFNTLTRRLEPFIPGGVPKENIEDYPPVTIYSCGPTVYSY
                     AHIGNFRTFVFNDFLRRYLKFRGFKVNHAMNITDVDDKTIAGALNDGITLREYTDKYT
                     NIFFEDLRTLNIEPVEHYPRATESIEAMIDIIAHLDKKGLIYEKDGSIYFSIAKFHRY
                     GRLSNVTSMDIKAGARYDADEYSKEDVRDFALWKAPKENEPYWDTPFGKGRPGWHIEC
                     SAMVRKIFGTTIDIHTGGVDLIFPHHENEIAQSEAAYDEPFVRYWIHVEHLLVEGSKM
                     SKSLGNFYTLRDLLDKGYSPRAIRYLLLTAHYRKQLNFTFDGLNQAAQALLRIDNLLA
                     RLNDIKHDALPNQKVTNRCNAFITAFTETVDDDLNIAGGTGIFFDFIHDINTMIDSNS
                     LCKSDAMYVMDVLKKIDTVFGFIFFPEVLHLNKEEIEALIEERNKARKEKNFAKADAI
                     RDELLQKGIILQDTKEGTRWIRKS"
     gene            complement(15881..16390)
                     /locus_tag="PLV81_05935"
     CDS             complement(15881..16390)
                     /locus_tag="PLV81_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09676.1"
                     /translation="MLFKRKKKTLPDVYEFTDDDGKRWLQTSTKDLLSSKELNDIIKE
                     IEKEKDEGEIVIARIDFKKVNKEYYERIVELEEKLKKRTELLKRVVKESRETIDRKNK
                     KMKELIEYIKKLHLLLAYYKLSPEDFNKIDFTKAYIPAPEMPAPPPSIEVKEKTIEYV
                     PVREEIIQD"
     gene            complement(16501..17784)
                     /locus_tag="PLV81_05940"
     CDS             complement(16501..17784)
                     /locus_tag="PLV81_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09677.1"
                     /translation="MIPLLKKITYSCLCVLFSVSMLNAKTLTVVFPFCPEDLNDQSSI
                     LFESLKNMTIEGVSIDSIQNVSSLHSMLDLNDDGARLSIKKFSDKRKSDYVLFSELSH
                     SVGQINIEYKLYSQENNNFIATSFDWALYNSIHLSAKTLEKQLSLILQTKIIKISNVL
                     ALGDSSLNQITINFEAYGNSSSYAILRSLLKDGPYDTIATISTTTYTDTDVQPGTVYW
                     YKVQALQDGFAIDSSTPVSAYITSENKENLDLDKVLSQYKKPEEKPQDEEQKALIKEH
                     IDMIKPQYMNSVKLRIIMQVVKSYISRGQLTVLKDFDTVIMDTENNVVYFIKKNTYVV
                     KLETKKPFDLLTTSNYDMELVQRLLLNAIAFGAQDGTIEVTDDEGYTAQLPLYQSIGI
                     ATTYFKEYKNWAGNTILWSTSNKEIRAKMKEYSNQ"
     gene            18046..18486
                     /locus_tag="PLV81_05945"
     CDS             18046..18486
                     /locus_tag="PLV81_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09678.1"
                     /translation="MGTYEHLIVRKPINVNELPDHDFSQVIPYPVLMGKELVPEANAW
                     ALYLYIKTITQEMKDIAIKMDRATPHKHDFDEMYLMIGDEKAITFEVMLGDETYQVAT
                     PAAVYIPKGTPHAIRPVDATIGATGGLIPVCLNGEYITLPVDKG"
     gene            18490..19806
                     /locus_tag="PLV81_05950"
     CDS             18490..19806
                     /locus_tag="PLV81_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOM09679.1"
                     /translation="MNPHEDIKAMYSKAIEAASPYGAVRSSLIREGNTLRLKDGSVFD
                     LSAFKHIYVVGAGKATAPMAKAVEDILGDSISEGVIAVKYGYREKLSHITLREAAHPV
                     PDENGLKASQEIIDLLGKAGGDDLVISCISGGGSALLPYPVKPITLAEKQDLTQKLLK
                     SGASIKELNIVRKHLSRTKGGNLALAAYPATVINLMMSDVVGDDIDIIASGPFVPDES
                     TYSEALSILQRYKLEKTVAPSIIHHLEKGVNGEVPETPKDKHIFEKVHNSIIASNIIA
                     CMAAYKKARDLGYNSLILSSMIEGDTAQAAGLHSAIAHEILKSGNPLEKPACIISGGE
                     TTVVVKGSGLGGRNMEFALQIALLIEGTSIVAASVGTDGTDGPTDAAGALADGTTVKR
                     AQKLGLNIEEYINNSDSYHFFEKLGDLIKTGPTNTNVMDLRIIIVV"
     gene            19942..20667
                     /locus_tag="PLV81_05955"
     CDS             19942..20667
                     /locus_tag="PLV81_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09680.1"
                     /translation="MKKKSILFVLAIALVLSLQFTPMVKAQQAQPAEKQQFTEATRPE
                     ENYLARFHALERVEFLKKSNLDKIYMLKVIITNYKDQGWDKDYQAIYEGYKKGMDYYY
                     KRDIIYAQVELEKNRKDIFELYKKIAEVYKQRTQALLNECATKILDLDLDERSRSNPD
                     RNKVLFKNVARLRIAYGQFDDGQNMMDDHVYDTAVKHFRVAKSYAIQILEDIDPENAK
                     GKYDLDKADNLNRILNPPQQSAK"
     gene            20769..22121
                     /locus_tag="PLV81_05960"
     CDS             20769..22121
                     /locus_tag="PLV81_05960"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOM09681.1"
                     /translation="MANVDCLQKIVVFLVVITITIFSFKIPITRLEFPLYRVVLDPGH
                     GGKATIPKDEYGDRFDVLSMKYLDVYREGASYKDYHEHIYTYEIAKRVDALLQLLSPN
                     GNFEKFYTILQKYTDKPVKRIYIQTFMSRGPSLNSHLIHKEPNAPYRLFDFISNDGTL
                     KEGRISYINSIKPHLVVSIHFALNSSPYFRGMNAVIAAPYSILYKGLQYFQGTLTDRS
                     FFYHSKYADWFTEDERKSGFFWYSNDVVMYFTGYRIKNDYSINTDAFRGYRYNMVQWA
                     FSDPPGWAHIAKHHPPKTPYANNIQQFIPQNAFFTREQSKYEQYRRDGGFEGYGGDNL
                     YASNEIIRFVLYNLYSKGIRHKDQRLAPPYISIWSVPLHINAINAFIEFGYLARPYTR
                     TIINNHLDDVAEGIAVGIYSLFAGVEVTKKYPYTPYGKKIDLEKYKIDNDNDYFTIVR
                     "
     gene            22153..23421
                     /locus_tag="PLV81_05965"
     CDS             22153..23421
                     /locus_tag="PLV81_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOM09682.1"
                     /translation="MKKLYAIIILFFSLSSAYAQDTVSEKFVAAAQKASPSVVSIQVF
                     VVEKGHYRKVGYASGTILSSKGYVVTNYHVVSKGTVYQINLADGTECEVESFSDGSIY
                     RVDEKTDIALLKVRHPAGIPLQAIEFEDEKVYPGQWVLAIGNPFGLQQSVTGGIVSSV
                     GRSDIGFADIEDYIQTDVPINPGNSGGPLVSLNGRLVGINTAIRTQTGGYQGISFAIP
                     ASIVKQVVGELIQYGRVRRGWLGFLVQEKFIKDGAQTQVEVISVVDGSPASRAGIEVG
                     DIVRRVDGVQIKKISDLVKSIHAKQVGSPIELVIARDGQLLTYNFVLHEKTTAKKGNQ
                     WLTALKSRYGITIDDARNGVVIVEVLQFGLAYNVLKKGDIIQSIDGMGIASLQDVIAV
                     LKKNNGVIQNCIVLRGGRVIRCSFNSQEVE"
     gene            23424..>23686
                     /locus_tag="PLV81_05970"
     CDS             23424..>23686
                     /locus_tag="PLV81_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09683.1"
                     /translation="MKWIYSTILFLLCAIVTVHVTADEAKEVVLDTQWYTSLQNSDAI
                     KRLQMLDALKDKVVKAQGQVIKVEESNLLKKKYRIILSVNLPK"
     assembly_gap    23687..23786
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23787..24214)
                     /locus_tag="PLV81_05975"
     CDS             complement(<23787..24214)
                     /locus_tag="PLV81_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09684.1"
                     /translation="MKKITRTNDIKTNTPIRVYKKGYGYTLLTLIENNDLFLICKSPE
                     DFFTYIPENETVECYIWPYPDGSYDFTTTVLGKISSPFPIILLKHTDSVVYNPTRQCL
                     RAKVNIPFTFFTFSINTTKSFSSEDIQWHQGTILELTDREA"
     gene            24565..24858
                     /gene="rpsF"
                     /locus_tag="PLV81_05980"
     CDS             24565..24858
                     /gene="rpsF"
                     /locus_tag="PLV81_05980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOM09685.1"
                     /translation="MNKYELIVILRPSDKLESLKEKVREILKKNQVVIESDEPWGIRS
                     LAYTIDRANDGYYEYFIAQINPTAVSNIIAEFRLQPDILRYLFVKLPTLSKTA"
     gene            24864..25286
                     /gene="ssb"
                     /locus_tag="PLV81_05985"
     CDS             24864..25286
                     /gene="ssb"
                     /locus_tag="PLV81_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOM09686.1"
                     /translation="MAGDLNKVILVGRVVRDPELRYTPGGSAVVSFSVANNRIYVKNN
                     EKQEVVSFFNCVAWGKLAETVANYCKKGQQLALEGRLQQRSWQDKEGNKRSTVEIVAE
                     SLQFLGGRQQSDSQVTIEQDEPVDINSDQPFADDEIPF"
     gene            25318..25665
                     /gene="rpsR"
                     /locus_tag="PLV81_05990"
     CDS             25318..25665
                     /gene="rpsR"
                     /locus_tag="PLV81_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOM09687.1"
                     /translation="MSEEIKHDETIEAQDNVNPQEVKPEAVAPEEDDKVLATAVQVQR
                     IPKFKKKVCRFCEDPNLAIDYKNPDLLVRFITDRGKILPRRITGTCAKHQRHLARAIK
                     RARIIALLPFVVK"
     gene            25683..26531
                     /locus_tag="PLV81_05995"
     CDS             25683..26531
                     /locus_tag="PLV81_05995"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HOM09688.1"
                     /translation="MELLLHFILGIALSVVIAMLVYKLQSQLGAIALIIIATASYIGL
                     SYLVNEMIGIQFIIAGAIFGYTAQRFSFQKYFMTSVLVLWFSLLVVYYLQYYLKGVDV
                     VADNHAMIQQMIEGSSLGAAEKSVMMQQLENFMPTMRILMPFSYFFSSIVISLFIYVL
                     VYAFFIRFKFLTNSNSTFALFRINEWTVFLLIASWATVLLSSSDHIVWAIALNVGLIL
                     CVVYTIQGLSIVSFYFEKKQWPKLYGVVLLFVGILLGFQVLFFIITMLMGLGIIDFWM
                     NFRKIV"
     gene            26554..26997
                     /gene="rplI"
                     /locus_tag="PLV81_06000"
     CDS             26554..26997
                     /gene="rplI"
                     /locus_tag="PLV81_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017851930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOM09689.1"
                     /translation="MKVILQKDISNLGEAGDVKEVAEGYARNYLLPKKLVIPYNESSA
                     KAIEHQKKLVKIKKEKRKKEGQKLVEALSNVALTIGAQVGEEDKLFGSVTAIDIAQKL
                     SHMGYSIDKRKIVLPEPIKKAGEHEVKIKLDEGLSATIKVTVVKE"
     gene            27002..28330
                     /gene="dnaB"
                     /locus_tag="PLV81_06005"
     CDS             27002..28330
                     /gene="dnaB"
                     /locus_tag="PLV81_06005"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOM09690.1"
                     /translation="MLDAGMPPHDVEAEAACLSSILLSKDALLKVIEILQVEDFYIDA
                     HRMIYEAIRELEIKAIPIDLITIKQRLIDKKQFEKAGGDPALVNIYRTVSTAANAEFY
                     ANRVKELSLRRKLIETCTTIAEKSYDTTLDTKILLDDAEKDIFLITQRRITSDFESLA
                     SVLKQTLEDIGHMYETKRPVTGIASGFTDLDAMLTGFHESELIIIAARPSMGKTALAL
                     NIANHVALREKQPTLFFSLEMPATQLATRILCIESMIDMQHVRTGQISSEQLRKLIDV
                     SGRLEKSPMFIDDTPGLSIFELRAKARRFAQKQKVGLIVVDYLQLIVSNNRLERHQQI
                     AEISNALKQLARELSVPVIALSQLSRAVESRTDQKPQLSDLRESGAIEQDADVVLFIY
                     REEKVKKETERIGIADIIVAKQRNGPTGTIELRFWDKYMKFGNVEKFRED"
     gene            28332..29117
                     /locus_tag="PLV81_06010"
     CDS             28332..29117
                     /locus_tag="PLV81_06010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOM09691.1"
                     /translation="MNGTEITIPRTVVQFFESVGKHSLFFTQTVKWIFKPPFDAKNIL
                     TQMLEIGYKSLPVTCITLFFTGMVMALQIGKAMDSLMSGSSVFIGSAVSIAMFRELCP
                     VLTALLLSGRVGSAIAAEIGTMRVTEQIDALITLDANPIQYLSVPRFLACLVMTPVLV
                     VVTDVVGSLGGAFIAYFNLGITIDKYVENVFNYLGVMDFLTGLFKSVFFGIEIAIIAC
                     YQGFNTSGGAEGVGRATIRAVVASSMTILISDYFLTYLLQFIE"
     gene            29134..29778
                     /locus_tag="PLV81_06015"
     CDS             29134..29778
                     /locus_tag="PLV81_06015"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOM09692.1"
                     /translation="MKSLKKRKEEFIKFLQKNKIPKKVLDAFVAVNQQDFFDEIYEDR
                     LYGQLPIPIGRGETSDEPLALARMIQYCGIKKDFHVLEIGTGSGYSTAVLSQLAKSVT
                     TIEIDEELAVQAKQRFKKTGLSNIRIFQGDGTDIDESLGFFDAIIVLAGCKKRPLLLL
                     HVAKPDSMIVFPMGPAHQQQIALMLNHRDDNVEGLYPVVFKEFCSFMPIIGIYG"
     gene            complement(29824..30168)
                     /locus_tag="PLV81_06020"
     CDS             complement(29824..30168)
                     /locus_tag="PLV81_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09693.1"
                     /translation="MIETILYILSFGAGIAVFLYLLSLYHHIKERPKPDNKQPAQKVT
                     QDKIQFKSQPVNEPVHLKERTCPVCGSKLSKYEALYASKIVKDSTKILIYGCRYCYKP
                     QEDPDKKKKSDI"
     gene            complement(30165..30893)
                     /locus_tag="PLV81_06025"
     CDS             complement(30165..30893)
                     /locus_tag="PLV81_06025"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOM09694.1"
                     /translation="MKPYHYLAPYYDYMLKHVDYATWYHYLRMIMCRYINNPRTVLEL
                     GCGTGKFGPKFSMDDYEIYGMDKSLEMLKIAKARAYKHYHVFCGDITQFALSKTIDFI
                     FSVHDTFNYILDYDDFVSALHCVHSCMNNDSIFLFDLTTEYNIKKNFHNKLLTFQVKG
                     TDIQWYNEYDKHSKILYSKLTFTRHTNTLVEEHLQRLYTIDEVLPLIEQCGLTVVDVV
                     GDYTLKPPHEETIMVNFITKRAQR"
     gene            complement(30890..32053)
                     /locus_tag="PLV81_06030"
     CDS             complement(30890..32053)
                     /locus_tag="PLV81_06030"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOM09695.1"
                     /translation="MNLKDKLNIYYTDSGKPVTDSSWIQDAGGEIITTPFGQTITFVQ
                     QYPVASLHIDDTVTDIAAYCAMHRMEPVTLDQCVFFDIETTSLSSGTGNYAFLIGLGY
                     FSENKFTVEQIFMRQYGEEPAVLFHCAERLQKFKTIVTFNGKTFDIPIIKTRYRINRI
                     QGFPVSMPVIDLLKPARSIFKSIYASCSLKSLEELLLGITRTDDIPGYQIPDVYFTYQ
                     QTGYDPRLTAVIEHNRIDITSMVLLLVFFNSLYTLIHTKQFDQVPTHLYKNIAKHLYS
                     RNIQLFIELIEYAGYALLDNDHSLFKKFSTALKRSGLLEQALTFWEKHINLFSLEELA
                     KHYEHRVKDYHKALSYCTWATAHSKELSDNQKLIEHYTERFTHRINRLNRKIQ"
     gene            complement(32016..34295)
                     /locus_tag="PLV81_06035"
     CDS             complement(32016..34295)
                     /locus_tag="PLV81_06035"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013916657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOM09696.1"
                     /translation="MTIEQLIEYLKNYEPFMKQVAAWYTVPARQAVYAPYPDDIDERI
                     RNVYMQRGIENLYSHQRSAYDAVQKKENIVVVTPTASGKTLCYNLPVLNFMAKHPDAR
                     ALYLFPTKALSQDQLSELQEIMKITGLSIKTYTFDGDTPQSARKAIRTAGNIVITNPD
                     MLHSGILPHHTIWVKLFENLQYVVVDEIHTYKGVFGSHFANLMRRLKRICSFYGSHPQ
                     FICCSATIANPKELAEKLFEMPFTVIDNNGAPCGKKHFVIYNPPVVDKALGIRASAVK
                     EAAKVGTLCVKNNISTIVFCRSRLRVEIIATYLKEQCKGFSVAAYRGGYLPNERRSIE
                     KGLREKKINAVVSTNALELGIDIGMLDCAISVGYPGSIASLQQQSGRAGRRGTDSLAI
                     MVATSSPLDQYIATNPHFVFENNPEMANINPDNLLILMDHIKCAAFEIPFGIDELFTP
                     HHSTTREMLEYLAQKNILKESAGKFHWMSDIYPANDISLRSASHDNVVIVHKDNHAQV
                     IGEIDLPSAPTMIHDDAIYLHQGQQYYIDKLDWERRIAYCHEVESDYYTDAEEKTDIH
                     VLEEFESKPLSIATLSLGEVNVRTKAIMFKKIKFGTHENVGWGKINLPELEMHSTAMW
                     CSFSDEIINTMEREEAGEVFLGLSYILRNIAPLYTLSDYADIRVVFENRSHFTNQPTI
                     FVYDAIPGGVGISERVYHIFGLIAQASQEAIAACSCTHGCPACIGPMITREGIKEKVI
                     ALLKNLHHESEGQTQHILH"
     gene            complement(34292..>35134)
                     /locus_tag="PLV81_06040"
     CDS             complement(34292..>35134)
                     /locus_tag="PLV81_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HOM09697.1"
                     /translation="EQSLELSHKLLLQPLLQKVTIPISEYSFANLYLFRSVHQYILIT
                     GDITAIKGVTYDGIHYIMPVMPLDEEYAEKLVQLLQPNMCLFPIHHDWLHFFTEKGFT
                     YHYNDGDSDYVYTKEKLATYSGKKLHGKRNLLYQFTAKYSVEVLPLTSQRVNDALYIL
                     DIWKKESGNDADIAACIEACMLLDELNLCGSMYYVEGKPAAFIIGEAITDDMFAIHFA
                     KAITSYKGIYQYMYNHCAKALPANYKYFNFEQDLGLEPLRFAKRSYQPDMIVTKYRVS
                     ALIQ"
BASE COUNT        10753 a         6153 c         7507 g        10621 t
ORIGIN      
        1 tggtatggtg gatatactgg cattaaaaaa tcaatgtttt ttctactaca attacaccac
       61 cattcctgca tcaagcactc cggttgcttt gcctttatcc actacttttt tgccgtttat
      121 gaacacataa tcaatgccag atggccgttt attggtttcg gtcaatgtgt tgttatcttt
      181 tacgtttttc cagtcaacga tggtaatatc agccatataa ccttctttca ggtaaccgcg
      241 ttttttcagc ataaaacggt cagcaatagc accggtcatt tttcgtatta attcctgggg
      301 cgatagtaac tggtaatcgc gggctatttc aaagaaacga ggatagttgc caaacgcagc
      361 cggattctga gcaccctgtg aatacggtgt ggcgtcggtc ataaacagtg atgcgcgatg
      421 cttcatgaga tccagtatgt tttgaaaact tgaatagcgg tgattgagta cacgtgcacg
      481 accgttggat ttttttgcaa aatcaataaa attttcatac tggctcatgc cacgttcttt
      541 tgcaatgtct tttaagaatt tgccattgta tttcgcaagc tcaggattgt tggcattgat
      601 aatctgaata tcgttatagc caaaaccaag caatgcaaca atcaattcta actgagcgcg
      661 aagtttaaga agagctactt tatcttcata tattaagaaa ctcttttcgt gtctgttcgt
      721 tatcaaatat agattcatta aatttcatgg caacctccat ggtaatataa aatcttaaaa
      781 tttagattaa agtaagaact ttcacaggtt atttcaagaa gataatttaa aatagtatgt
      841 attttttaac attaatggat gaaggatgga gtgtacactc caactgcaga gagtggaggg
      901 aatgtacact attacatatt tgttcataac catactacat aaaaaatttt ttataaacta
      961 cagaaaattt ggttgaccgt tcaataggta tgtaatacag taataatgta atacataata
     1021 aatatgcatt aatatattta tgcatataca aattattatt tatattgatg gaggattcaa
     1081 tggcaaaggg tgacagaatt gcaataattg atggatgccg tacgcccttt ttacgctccg
     1141 gtacagatta ccgggaaatg atggcgtatg aaatctgtcg tcatgcggtt aagggcctga
     1201 tagcaaaaac aggcattccc aatgacctgg tagaccatgt cattatgggg ggtgttgcaa
     1261 atgatattgc aaccacaaat gttgcgcgtg agattatgct ggcagcaggg cttccctata
     1321 caatacctgc atatacctgt acggtggcat gtatatcggc aaatgctgct attatcagtg
     1381 gagccaattt gattgtgaat ggtaatgcgg agtgtattgt tgcgggaggt gttgagactt
     1441 tttcagatcc tgatataaaa atatctaaaa actatcgccg atttatttta gacctcacca
     1501 tgttcaagcg cccaaaggga ataattggca aactgaaatt attaaaaggc atgagcttta
     1561 aggattttat agttccccaa caacctgcaa ttggtgaatt ttctacaaaa atgcgtatgg
     1621 gggacacagc agacaggctg gcaaaacggt taggtatcag tcaacgtgaa caggatgaat
     1681 ttgcagcact atcgcataat cgtgctgcag ctgcctggga aaggggggtc ttgaaaaaag
     1741 aggtggtacc ggttgtaaca ccgcagggga agctagtgga aaaagacaat ggtttccgta
     1801 aggatacaac cccagaaaaa ttagcaaaat taaaacctgc ctttgacagg cggtatggta
     1861 cagtaacggc aggcaattcc tcatttttga cggatggtgg ggcagcggtt cttcttatga
     1921 gtgaaacaat ggcaaaaaag atgggaataa aaccactggc atatatcaag tcatatgctt
     1981 tcacagcaca ggatttgtgg gaagaacttc tactggggcc ttcttttgca ataccaaaag
     2041 cattagcaat gggtaagctc aagtttaaag atattggagt atgggaaatt catgaagctt
     2101 ttggtgccca gatgattggt gttattaaaa cgcttgcatc cgcttctttc tgcagagaca
     2161 ggcttgggtt gccgggcaaa ttgggcgaaa tccccattga tgcactcaat gtatatggcg
     2221 gttccctttc attggggcat ccgtttggtg ctaccggggc acgtcttgtt actacatgtg
     2281 ccaacaggtt gcgggaaact ggcaaacgat atggcgtggt tgccgggtgt gcagccggtg
     2341 cagtgggcaa tgccatcata ttagagcgtg cataaaggag gaatgaccat gaaagatctg
     2401 cagtttttaa aaactgaagt tatcaatgat gtgctggtgg taagcatcaa ttgccccggc
     2461 aaggtaaata aagtttccag tgatctttta cgtgaaattg aaacaataat taaccagact
     2521 aaaggcaaac catataaagg gatggtaata ctcagtgaaa agccagacaa ctttgttgtt
     2581 ggggctgata ttgatgaatt aaaagcaatg aaaactgatg aagagattaa gcagtatatc
     2641 tctaaagcaa atgatattct gttcaagtta gaaaaacttc catttcctac tgtatcaatg
     2701 attaatggca actgcctggg tggcggtgtg gagcttacgc ttgtcactga ttaccgcatt
     2761 gctactgatt ccactgacac agtgatgggc ctgcctgaaa ttaagttagg ccttatccct
     2821 gcagctggcg gtacacagcg ccttccccgc cttattggca tacagaatgc actgccactt
     2881 attttacagg gatccaatgt gcgaccaaga agggcaaagc gcctggggct cattgatgag
     2941 atagttatac catatggatt aaaagagata ggcgttaaaa aggcattaca gttaaaggga
     3001 aagcgcacaa taaaacgcaa aaggaaattt gttgaatggt tgcttgaatc aaatcctctg
     3061 ggccgaaaga ttatcttttc acaggcccga aagatggtca tgaagcaaac ccggggatta
     3121 tatcctgcag cgctggcagc tatagattca gttgagtatg gttacaaaac caatgttaca
     3181 aaaggtgtgg caaaagatat agagcgcttt gtgactttag taaaaagtta ccagtcaaaa
     3241 tcattgatga atcttttctt tgcaatgacc gatctcaaga aaaatccatt agagaagaaa
     3301 gcaaagaata tttcaaaatt agcggtgata ggcaccggcc tcatgggtac cgggattgct
     3361 caggtttcgt tgccgttgtg tgatactgtg cttgttaagg acatggcgct tgatgcagtg
     3421 tcaaggggta tggcgcagat tcgcaagagt ttagaaaaac gcgctcgctc tggtgccata
     3481 cgggattttg ataaagaaat attatggggc aaaatagttc catgtgatga ttatgtgaat
     3541 tttaagaata ccgaccttgt cattgaagca gtatttgaag acctgcaact gaaacagaac
     3601 ctggtaaaag aggttgaagc agttaccggc gataacacaa tttttgcatc aaatacatca
     3661 gccattccta taagcctcat tgccagagct tcaaaacgac cacagaatat gataggtatg
     3721 cattattttt ccccagttcc tgccatgcca ctgttagaaa ttataaagac tccaaagaca
     3781 gctgattggg tagtggcgac agctctaaaa tttggcattg cgcagggcaa aacatgcata
     3841 gtggtcaagg atggcccggg attttacaca acgcgaatac ttgcaccata tctcaatgaa
     3901 gcggtgttct tgattgaaga aggtgtgcca atgaatgata tagaccgagc cctgttgcag
     3961 tttggctatc caattggtcc tgttgcactc cttgatgagg ttggtattga tgttggcgca
     4021 catgtggcaa ttgagctggg gcaggaattc aaggcacggg gtgcagcacc gtccaatgta
     4081 ttgccacttt tattcagtaa gggatttaaa ggtaaaaaga ataagaaagg gttctatgat
     4141 tacagcaaac caaagaagaa aggtatgcgt gtacccaacg aagaaatcta caaggttata
     4201 ggtaaagtaa cctggcagca tttgccaatg caggaaattc aggagcgtgt aagcctcatg
     4261 atgattaatg aggcagccct gtgtctgcag gagggtatta tatcttcgcc gcgtgacggt
     4321 gatgttggtt ccattttagg attgggtttt ccaccattca gaggaggaat tttccgtcat
     4381 atagaccggg aaggtgtcaa aaccatcgtt gatacaatga tgaaatatta tgataaaggg
     4441 atgaaacgtt ttaccccggc acagatactt gttgatatgg caaagagtgg caaacgcttt
     4501 tataaggagt aaacaatgaa gcgttctata caattttctc aggaacatga tatattcagg
     4561 caaacagtac gcaaattttt tgaaacagaa gttatgccgc attacgacaa atgggaagaa
     4621 gaaggtatag tgccacgaca gatgtggaag cggagtggag aattggggtt actacttccc
     4681 tggctggatg agaagtatgg agggcctggt ggtgattttt tgtattcggt gatagtgagt
     4741 gaggaattag cctatagtgg ggctaattca ttcatgatac cgttacataa tgatattgtg
     4801 gcaccatata tctattcatt tggcacagaa gaacagaaga tgaagtacct gccagggtgt
     4861 gcgtctggcg atataattct ggctgtagca atgaccgagc ctgaggctgg ttcggattta
     4921 gcggcaatga aaacgagtgc aaccctggat ggtgatagct gggtactcaa tggtcagaag
     4981 acatttatat ccaatggtat attagctgac cttgtagttg tagcagcaaa atcgtcaaaa
     5041 gaagcaccac cgcatcaggc tgtaagcctt tttctggtgg aaaacggtac acctgggttt
     5101 acaaagggaa aagcaataaa aaagataggc ttaaaagccc aggacactgc agagctattt
     5161 tttgataact gtcgcatacc aaaagaaaat cttcttggtc aggaagggtt gggatttatt
     5221 taccttatgc agaaattaca gcaagaacgg ttggtatgtg ctttggcgtc acaggcagca
     5281 atggaaaaat gtttagcaat aactatcgac tatgtaaaag aacgcaaact ttttgggaaa
     5341 ccattgtcaa aattccagaa tacccagttt gccatagcag agatggcttc tgaaattgct
     5401 gttggccgca gttttattga tgacctgata ctccatcaca ttcggggtga agatgttgtc
     5461 aaagaaacct gtatggcaaa gtactggata tgcgaaacag caaagcgtgt tgccgatcga
     5521 tgcctgcaat tttttggcgg ttatgggtac tgtaccgaat atcccgtgtc acgcttctat
     5581 gttgacgcac ggattcagac aatctatgct ggcacatccg aagtgatgaa gatgattatc
     5641 tcgaaaatga tgggattgta atttaatagt tgacattcat agcaaagtat agtgtaatcc
     5701 ttgcgttttt taaaatttgt tcaaaattta aaatttgttg aattgtggag gccattatga
     5761 atccagtgat acgcaagggt tacacatata tggcatggct tttcattggt gttgataccg
     5821 ctttctgggg aactatctcc ctgttttcct ggtttgtaag ccccaggggt aacctggcac
     5881 acttttgcat gcaaaactgg tcaaaaaata tactctggtt ttgcggcatt cgctatcagg
     5941 ttgaaggtct tgaaaatatt acgaaagata aagttcagat atttgcatcc aaccatcaga
     6001 gtttttttga tgtgtggcta ctgagtgcca ttattccggt aaagtttggc tggactgcta
     6061 aaaaggagct ttttaaaatc ccatttatgg ggtggcacat gacattaagc ggttacatcc
     6121 ctattgacag aaaaaacaga gagcgggcaa tcgaaagcat gaaacaagct gccattaaaa
     6181 tacgaaaagg taaccgcatc atgatattcc ctgaaggcac acgcagccgc acgggagtat
     6241 tacagccgtt taaaaaaggc ttgttttatt tgtgtattga aacaggggtg ccgatagttc
     6301 ctatttatat acacggtacc tatcagcggc ttaagcctga atcaggcgat attaatcccg
     6361 gaaaggtata tgtacttgtt ggcaaagaga tgccaaccga gcattatacc gttgatacca
     6421 ttgagcaggc aatggaagat ttgcgccaga gaattttggc tttacaaaat gagtgcctgc
     6481 agcgatatgg tgaagcggta taggtgaata ttctattctt tcaggagata caatgtatgg
     6541 aagaccttaa aaaagcatat cgcaccatca tggatgacca tttccctgat accatgacca
     6601 ttacctttgg caatcagaca cttgtatata gaaagcgaac gtggaagatt aatgatgaag
     6661 gacagctcat tgaaaagggt ttgcgctacg gtgaaaaccc ggggcaggag gcagcattgt
     6721 atgagcttgt caatggcaat cttgtattgg gcgattgtgc cttcattact ccgggacatt
     6781 cgcttgtaag tggcattgat gaatcgatga tgcttcagtt tggaaagcat cccggcaaga
     6841 ttaattttac agatattgac aatgcactca atatattaaa atacttaatg caaaagcctg
     6901 cagcagttat agtaaaacac aataacccct gtggcgttgc gtatggttca acaattgcag
     6961 atgcatataa cagggcaaac agggccgacc gcatagcagc ttttggaggc tgtgtggcat
     7021 ttaacagatc tgtcgacaaa gaaactgcac agctcatttc agctaattat ttagaggtac
     7081 tggttgcccc tgattttgaa ccgggggtta tggaaatcct tgcaaagaaa aaagacctgc
     7141 gtatagtgca gataaaacga atagacaggc ttgaagacta taaaacaatg cgctttgttg
     7201 attttaaatc acttattgat ggtgggatta ttgtgcagca atcaccaata aatattgtta
     7261 acacaaaaga agatttgaaa ccagcacaaa ctgaatataa ggggcagctt tacaaaattg
     7321 acaggctgcc aaccgaaaaa gaatatgaag atttattgtt tggatggttt gttgagcagg
     7381 gtgttacttc caattctgtg ttatatgtta aggatggggt tactgtagga attggtaccg
     7441 gtgaacagga tagagtaggc gttgcagaga ttgctgtgca aaaggcgtat actaaatatg
     7501 ccgatatact ttgctttgat aaatacggga tttcgtacta ccagttagca cttgaagtag
     7561 aaaaaggaat acgtaaagaa gccgataaaa ttgagataga tgaggcaaca aaagaagcca
     7621 aaggggggct tattggtgca agtatggtgt ctgatgcatt ctttcccttc agggatggtg
     7681 ttgatgtggg tataaagcag ggaattactg caattgtaca acctggtggc tccatacgtg
     7741 attgggaaag cattgttgca tgcaatgaag caaatccaaa agttgcaatg gtatttacag
     7801 gacagagggc gttcaagcat taaaaggcaa aagcgcatta cctgcgcttt tgctcatatt
     7861 ccttcatata ttcttctatt aagctaccgg tcttatgctg ttcttcctta acaggtttca
     7921 tgcgtcgtgc tgcaaaatca gctatagctt ttactggttt tttgaaaagg tcaacgataa
     7981 gttcaataaa atcgcgcagt cccgaaagta tagttttggt aattgaagtt ctggtgcctt
     8041 ttggggctgt tcgcatgggg gtaatactgg tagcaactgt atctgccaca ttttttacgc
     8101 catacatagc aggaatagga ttgtagctat ccttaatgta tgctttctgc ggtctgaaag
     8161 gtacccgttt aggtaggcgg cttgtcaata caatgtttgt tccagattcg gttgtcctgt
     8221 caatgatgaa aagaaaaata tagtaaatat acagaaaaag atttccaaag atggcaaaaa
     8281 agtgatacca gatatcaaga aaagcccaga atacatcagc ccatattttg ataaagtcca
     8341 ctatcttata aaagaaatta aaaaaattgt caacagtatt tacccagaat tgctttacta
     8401 ctctatagtt cataatagtt tcctcatgta aatgatttat tttactaaca actacgattt
     8461 gtataattaa atgctttatc gtcaataaaa aatcatactg ctctgacccc ttttttgttt
     8521 ttttcatatc tactcaagta tcatggaata ctttctacga tagtagtcct catactgctc
     8581 tattcttgtc cccctctctg taaacgtttt tccagttgca caaagtagtc atgatggtct
     8641 atgggtactc taacgtcgtg ttctcatata aatctgcaaa gatactgccg tagtggtatt
     8701 tgaaagggtc agagcataac tgttcaaagg ccaaacttga aattggaaga tttactgtgg
     8761 cactgtggaa aaaaaggacc agagctttct aatgctctaa tataggcata tattatattt
     8821 ttatacattg ctaaacaata tgtttatata tacaaataac gaatatataa atttttataa
     8881 agaaaagacc actggactga aaaaatattg aaatcgattt aaatattatt atattttttt
     8941 tagttaagta tctagtacct gagtaaatcc tatttaagga taagctattt atggcattaa
     9001 tagttgatat atttatggca ttgtctcatt cgattgatct agtggatcat aatattggaa
     9061 accacaataa acgagtagca tatatagcta gcagaatagc ccatgcaatg gggctttcta
     9121 ccggtgatat aacaagagta gttgttgctg gagcacttca tgatataggg gtattaaaag
     9181 agaaagaata taaagaattg gttcattttg attataaagg tggtattgat tatcacagtt
     9241 tgatgggtta ccgtttgctt tcttcctgta ccttaacaaa agatgtagcc gaaattgtca
     9301 aataccatca tgtttataat agtgaaaaga aaaatattcc tgaaaatcat acagttccaa
     9361 ttgaatcaga gattattcat attgctgacc gtatagatgt attgcttgat cacagtggtg
     9421 atttactgga acaaaaaaat aacgttattg caacacttga acaatattca ggtgatagat
     9481 tccatcctga tatagttaat tgttttatag atatagcaaa tcaggaatct ttctggtttg
     9541 atttacagtt taattcaatt gagaagaaga taaaaaattc tatctactat aatccaattc
     9601 tttccttaga agatattcat gaaatatcaa agctttttac caggattatt gacttcagaa
     9661 gccgctatac cgcaacccat tctacctctg tagcaatagt agcacgtgaa ttaggagcac
     9721 tgctgaattt atctgataga gaatgtttga tgcttgatat agccggtcac ctccacgata
     9781 taggaaaatt agcaatacca ctttcaatac tggaaaaacc tgatacgtta tcaccacaag
     9841 aatggcgcgt aatgaagcaa catacatatt ttacatttca catccttgat gaaattgata
     9901 gttatatatt taaagtgatc aatgaatggg cgtctttgca tcacgaaaag ttaaatggta
     9961 aaggatatcc gtttaaattt tctggtaaat atattactat gggttcacga ataatggctg
    10021 tagctgatat gtttacagca cttgcagaat cgcgtccata tcgtgatagc atggatgatg
    10081 atgccattaa gaaagaaatg aaaaaagcta aaggcgaaga acttgattca tatattgttg
    10141 atgttttatt gaacaattat tcatatttaa aagatattag acaaagagca tttgtaacgg
    10201 cattacaagg atttgaagat tgctttgatt atatttctaa aaaataaaaa tcaaaaattt
    10261 tatttgacaa tagttttttt tgacgtacta tttaactttg ccttttctat gtaagataga
    10321 tatacttact caggggtaat tatgaatgct atattccata aaatcaatgc atggacaata
    10381 aaggcaaaag ttactgctgg agtaattgtt gttcttattg tcagtttagc atttactaat
    10441 gtattctggc gtattggata tataaaggaa gttgagcgac agtttgttga taaggccaga
    10501 gcaatatgta ctatgggtga ggcattcagg gaatatatgg cagataattg ggaacgccag
    10561 ttatatgata aagattacct ggttaaagac attaagggta aatttgtgta ttctgttcct
    10621 gtattttcct ctattgttgc aatggaaaaa aaagcaaagg atcttggata tgaatttaga
    10681 gttcctaaag aatacccgcg caatccaaac aatattccca caccactgga acaaagaatt
    10741 ctcaatgaaa ttaagtcaaa aaatttaccc gaatatttca tgatagacta tgaaaatgat
    10801 gtcctgcggt atttcaggcc agtaaaactt acaaaagatt gccttatctg tcatggagat
    10861 cctgcacaat cgtacaagct gtgggggcga aaagatggca aggaccctac tggcggacca
    10921 atggaaggct ggaaagaggg cgaaatccac ggcgcattcc aaattatata ttcattaaag
    10981 gattactttg cagcacggtt acagatttta attataagca ttgtagtaaa tataattatt
    11041 ttaggtgtgg cgatagttat tatcagaaga gttgttcaca ggggattaca tcctcttgat
    11101 gaaatggtac aatctcttga agatattaat aaaggccagg gtgatttaac ccgcaaaata
    11161 gctattgagc gagatgatga agttgggcga cttgcactgc tttttaatca atttattgat
    11221 aatttacgta accttatctt agctgtaaag gatgcagccg atcatgtagc atcatcatca
    11281 gtagaaatga cacagtcaag tcagcagctt gccaacgtgg cacaggatca ggcagcttca
    11341 attgaagaaa catcttcagc aatggaagaa atcaaggcca ctatcgattc agtttctgaa
    11401 aatgcaaagg gacaggcaaa aaaagccgat gccacacgaa attctatgga atatctagct
    11461 gaagcaatag agcggattaa ccaaaatgca caggatgcat ataaaatggc tgatgaaaca
    11521 catggctatg caaaagaagg tgaagctgtg ctgggcagca ctgtaagtgg aatgcgggca
    11581 attaatgaaa gttcacgccg cattacggag atagttacta ttatatctga tatatctgat
    11641 caaattaatc tgttatcatt aaatgcatcc attgaagcag ctcgcgcggg tgaacatggt
    11701 agaggatttg cagttgttgc tgaagaaata tccaaactgg cagatcagac tgcgcagagc
    11761 tccaaagaaa ttaacaagct tatattagaa accaataata aagtaaatgc aggctcagag
    11821 cttgttgaaa aaacagccaa ttcactgaga aagattattg aaaacgtcaa gactactgcg
    11881 gtccttatgg aaaatattgc tcagtcatca caggaactca atcgtatgag caatgaagtt
    11941 aaaactgacg ttgaagaagt aaacaaaatg tctgaagaaa tatctattat gatggaagaa
    12001 cagagtgctt ctaccaatga aataatcaag gctattaatc aaataaatga agtaacacag
    12061 attgtgtcaa gtggctctga agagcttgct gcagcaagtg aagaattatc atcacaggct
    12121 gaaacattaa ataatattgt gaagcaattt aaagtataag gtgtaccctt attataatgg
    12181 aatactctac agcattacga ccatttatca tcaaacggct tgaacaggta aagaaagccg
    12241 atattcttgt aggcatacca tgctttaata atgaagcaac cattgccaat gtcatgacca
    12301 tggtaagcga tgggctgttt acatattata aagacttgcg gccggtaatc tttgtatcgg
    12361 atgggggttc tactgatgat accagggata ttgcccaatc aattgaatta aaaccatggc
    12421 aggagcgaat cattcagatt tatcggggtg ttgcaggcaa aggatctgca ttcagagcaa
    12481 tttttgaagc agctcgcata cttgatgttg atgcatgcat ggtggttgat tctgacctgc
    12541 gcagtatatc tccccagtgg gtacagcggc tactatcgcc cgtacttgaa aaaaaatacc
    12601 agtttgttgc accagtatat acgcggtaca aatatgatgg cactattacc aataatattg
    12661 tatacaatat aatacgtgcg gtgtttggta agcgtattcg ccagccaata gggggtgact
    12721 ttacgttttc aaaacggctg gcacaactgt atctggataa gccggtgtgg tctaccgaca
    12781 ttgcgcggtt tggcattgat atattcatga ctgtcaatgc aatcgtgagc catgtggaag
    12841 tgtgccaggc aaatttagga gttaaaatac atgatgccaa ggatccggct cagtcacttg
    12901 ggcctatgtt tgttcaggtc atagggacat tgttcaggct tatggagcac tatgaaccat
    12961 actggaaaaa tattgagggc agcaaaccgg tgccaacatt tggtgatatt gatatgattg
    13021 aacctgaggc aattgaggtt aatctacaac gtcttgttga tgaatttaaa acaggcttta
    13081 tacagtttaa tgctctgtac agggagatgt tcagtaacca ggtgtttaca tcccttaaag
    13141 attcagcgca catggatgta gaaagtttca agatgccagt tgaaatatgg gttaagattg
    13201 tatatgaact ggcagcaatc tatcatacct ggcaaattaa taaggtgcgg cttataaatc
    13261 ttatggtgcc actgtatttt gggcggatag cgtcatttat taatgaaacc atttcaatga
    13321 attcatttga agctgaagag gtggtagaac agcaggctca agtctttgag cattataaac
    13381 cctatttaat ggagaagtgg caggccacag gcaaaaatat tgaactataa tctattcttt
    13441 taatagttcc cacaacacaa tgccacaggc aaccgatgca ttgagcgatg atacctttcc
    13501 tttaagcgga atgcgtacaa ttatatcgca caaactttgt gttagctggc gcataccgct
    13561 tccctcactg ccaatgatta caactgcagg tttataggta gcaatagtgt tgattggttg
    13621 agtgcctcta tcgctggtgc caattaccca gaatcctgcc tttttagcat catctaagaa
    13681 tcgtgccagg ttaggtacct gcaaaactgg aatatgagca gtagctcccg ctgacgcttt
    13741 gatgatggtt ggtgtaatct caggtgcatg tgctttcgta acaactactg cactgccacc
    13801 aagggcctcg gtggttcgga tgatggagcc aatgttgtgt ggatcggtta tctcatcaca
    13861 ggccacaata acaccgtgac tggaagcaat tttttttaac tcacattgtt tttcttcctt
    13921 tttggggcga tagttcataa caagagctac accctgatga tgggcagcac agtgtgaatc
    13981 aaaccagctt ttgtttactt tctgtacagg gatatgttta ctgtgggccg cagcaataat
    14041 aacatcgatg attttcccgt gtgcagattc ggatacatac agtgttgcat ccccttgaag
    14101 ggttgtaagg tattcaagca ctaccttgcg gccaactacg atttgcgtat ccaccgggta
    14161 ccttctttgg tgtcctgtaa tataatgcct ttttgcagaa gctcatcacg gattgcatca
    14221 gcttttgcaa aatttttttc tttgcgagct ttgttacgtt cttcaatcag ggcttcaatt
    14281 tcttccttat tgagatgcaa aacttcaggg aagaagataa atccaaatac tgtatctatt
    14341 ttctttaaca catccattac atacattgca tctgatttgc ataatgaatt ggaatcaatc
    14401 atggtattaa tatcatggat gaaatcaaaa aagatgccgg tgcctcctgc aatgtttaaa
    14461 tcatcatcaa ctgtttcggt aaacgcagtt atgaaagcat tgcatcggtt ggtaaccttt
    14521 tgattgggta atgcatcatg ttttatgtca ttgagacgtg ctaaaaggtt atcaatgcga
    14581 agaagtgcct gagcagcctg attcaatccg tcaaaggtaa aattgagttg tttacgataa
    14641 tgagcggtta aaagcaaata gcggattgct cgtggactat accctttatc aagaaggtcg
    14701 cgcagtgtat aaaaattgcc cagtgatttt gacatctttg aaccttcaac aagcaggtgt
    14761 tcaacatgga tccagtaccg cacaaatggt tcatcgtagg cagcttcact ctgagcaatc
    14821 tcattttcat gatgaggaaa aattaaatca acccctccag tgtgaatatc aatggtggtg
    14881 ccaaagattt tacgcaccat tgctgaacat tcaatatgcc atccaggcct tcctttgcca
    14941 aatggggtat cccagtatgg ttcgttttct tttggtgctt tccacagtgc aaagtcgcgc
    15001 acatcctctt tgctgtattc atcagcgtca tagcgggcac cagctttaat atccatactg
    15061 gtaacattgc ttaaacgccc ataccggtgg aattttgcaa tactgaaata gatggaacca
    15121 tctttttcat agataagccc tttcttatct aaatgggcaa tgatgtcaat catggcctca
    15181 atggattcgg tagcacgagg gtaatgttcc acaggttcaa tgttaagtgt tctaagatcc
    15241 tcaaagaata tatttgtgta tttgtcagta tattccctga gtgttatgcc atcattaaga
    15301 gcacctgcta ttgtcttatc atcaacgtcg gtgatattca ttgcgtgatt aaccttaaat
    15361 cccctgaatt taagatagcg ccgcaaaaag tcattaaaca caaatgttcg gaagttacct
    15421 atatgggcat agctatacac agtggggccg cacgaatata tggtaactgg cggataatct
    15481 tctatatttt ctttgggtac accaccaggt atgaatggtt caagacgtcg ggtaagtgta
    15541 ttgaatattt taagtgccac cgtttttcct ccagaagttt ggtgatagta ctaacaaaga
    15601 aattgcagct actgtaaaca gtcaagtaaa agtgaaaaag tgcagcatta aaatatgtat
    15661 atgctctttg ccaggtgaat aaattactct gaccgctccc atatttacca aaaagatctt
    15721 tgcctttttc atacggattg tttattttat tataatgttc aaaaaacagt gtaagtgctc
    15781 tttttattac ttcggatttt gatatatttt cttttttttg gggacagccc cttttatttt
    15841 ttgtcaggag ttattaatac attttttcta tatacatatg tcaatcctgt ataatttctt
    15901 ctctgacagg tacatactca atggtttttt cttttacctc aatggaagga ggaggagcag
    15961 gcatttccgg tgccgggata tatgcttttg taaaatctat tttattaaaa tcctcaggtg
    16021 agagcttata ataggctaaa aggaggtgaa gcttttttat atattcaata agttctttca
    16081 tctttttatt tttacggtca attgtttcac gagattcttt gacaacacgt tttagcaact
    16141 cagttctttt tttcagtttt tcttcaagtt caacaatacg ttcatagtac tctttattga
    16201 ctttcttaaa atcaatacgg gctataacaa tttcgccttc gtccttttcc ttttctattt
    16261 ccttaatgat gtcattgagt tccttggagg acaaaaggtc ctttgtagat gtctgcagcc
    16321 atcttttgcc gtcatcatcg gtaaactcat atacatcagg aagtgttttc ttttttcgtt
    16381 taaataacat actcgttact gtaatatata ttcatgaaac tccagtttgg aagatactgt
    16441 gttctttagt atactatcga cactttatca acatctgttt atgtaaatat ttcaggcaaa
    16501 ttactggttt gaatattctt tcatctttgc tctgatttcc ttgtttgatg tactccatag
    16561 aatagtattg cccgcccagt ttttatattc cttaaaatat gttgttgcaa taccaataga
    16621 ctgatacagc ggaagctggg ctgtatatcc ttcatcgtct gttacttcaa tggtgccatc
    16681 ctgtgctcca aaagcaatag cattgagcaa aagcctctga acaagctcca tatcatagtt
    16741 acttgttgtc agaagatcaa acggcttttt agtttccagt tttaccacat atgtattctt
    16801 cttaataaag tacacgacat tattttcggt gtccatgatt actgtatcaa aatcctttaa
    16861 tacagtaagc tgccctctgg aaatatatga tttcacgacc tgcatgatga ttctcaattt
    16921 aactgaattc atatattgcg gcttaatcat atcaatatgt tcttttatca gagctttctg
    16981 ttcttcatcc tgaggttttt cttcgggctt tttatattgc gaaagcactt tatccaggtc
    17041 aaggttttcc ttattctctg atgtgatata cgccgataca ggtgttgagc tgtctattgc
    17101 aaaaccatcc tgtaaagcct gaactttgta ccagtaaaca gttccaggct gaacatcagt
    17161 atcagtatag gttgtggtgg aaatggtggc gatagtatcg tacggtccat ctttcaaaag
    17221 cgaacgcagg atagcatagg aactgctgtt gccataggct tcaaaattaa tggtgatctg
    17281 atttaaggaa ctatcgccca gagccaatac attgcttatt tttattatct ttgtttgcag
    17341 tatgagcgat agttgttttt caagagtttt tgcagaaaga tgaatgctgt tatataaagc
    17401 ccaatcaaat gatgttgcaa tgaaattgtt attttcctgt gaatacagtt tatactctat
    17461 gtttatctgt cccactgaat gcgatagctc tgaaaaaagg acatagtcag atttgcgttt
    17521 atcggaaaat tttttaattg ataaacgtgc accgtcatca tttaaatcca gcatggagtg
    17581 caatgaacta acgttttgaa tgctatctat tgaaacacct tcaatagtca tgtttttaag
    17641 tgactcaaat aatatggaag attggtcatt caggtcttcc gggcaaaacg gaaagacaac
    17701 tgtcagggtt tttgcattaa gcatgctgac tgaaaacaaa acacataaac atgaatatgt
    17761 aatctttttt aacaatggta tcatactgtt cccttatctc ttgtatgtat atcgaatagt
    17821 aaccaaataa tagttatagt taaataaagt ataactactc actcatcctc aacgttcaac
    17881 ggttttacaa tgtagcaatc tgtattatgc cattcatcag taaagtgttt gacattaata
    17941 gtatcaacta tcatactaaa tatactaaaa tagtgtatgt aaatgcaact attttgttat
    18001 gaatttattg taaatacaat gttatacacg tattagggag gatgtatggg cacgtacgaa
    18061 cacctcattg tgcgaaagcc aataaatgtt aatgaacttc cggatcatga tttcagccag
    18121 gttattccct atcctgtact tatgggtaaa gaacttgtgc cagaagctaa tgcatgggca
    18181 ctgtatctgt acattaaaac aattacgcag gagatgaaag atattgccat aaaaatggac
    18241 agggcaacac cacataagca tgactttgat gagatgtact taatgattgg cgacgaaaaa
    18301 gcaattacct ttgaggtgat gcttggtgat gaaacgtatc aggttgcaac tcctgcggcg
    18361 gtgtatatcc caaagggcac tccgcatgca atacgtcctg tggatgcaac cattggtgcc
    18421 actggagggt tgataccggt gtgtctcaac ggtgagtata taacattgcc tgtggataag
    18481 ggatgaaaca tgaatccaca tgaagacatc aaggccatgt attcaaaggc tattgaggca
    18541 gccagtccct atggtgctgt acgttcgtcg ttgatacggg aaggcaatac gttgcgttta
    18601 aaggatggca gtgtgtttga tctttcagct ttcaagcata tttatgtggt tggggctggg
    18661 aaagcaactg cacccatggc aaaggcagtt gaggatattc tgggcgatag tatatcagaa
    18721 ggggtgattg ctgtaaaata tggttatcgt gaaaagcttt cccatattac cctgcgtgaa
    18781 gcagctcatc cagtgcccga tgaaaacgga ctgaaagcat ctcaggagat cattgatctg
    18841 cttggaaaag cagggggaga tgacctggta atatcatgca tttcaggtgg aggatcggca
    18901 ctgttgccgt atccggtaaa accaattaca ctggccgaaa agcaggattt aactcaaaag
    18961 ctattaaaaa gtggagcttc aattaaggaa ttaaatattg ttcgtaaaca cctatcacga
    19021 acaaaaggtg gcaacctggc gcttgcagca taccctgcca cagttattaa cctcatgatg
    19081 tcagatgtag tgggtgatga cattgatatc attgcatcag gtccatttgt gcctgatgaa
    19141 agtacctatt cagaagctct ttcaatatta cagcggtata agctggaaaa aacggttgct
    19201 ccatcaataa tccatcatct tgaaaaaggt gtaaatggtg aggttcctga aactcccaaa
    19261 gataaacata tctttgaaaa agtgcacaat agtattattg cttcaaatat cattgcatgc
    19321 atggcagcat acaaaaaggc acgtgatctg ggatataatt cactgatttt atcatcaatg
    19381 attgaaggcg acactgctca ggctgcaggc ttacacagtg caatagctca tgaaattctt
    19441 aaaagcggta atccacttga aaagcctgca tgtattatca gtggaggtga aaccaccgtg
    19501 gtggttaaag gttcaggact tggtgggcgt aatatggagt ttgcactaca gatagccctg
    19561 ttaattgaag gaacatcaat tgttgctgca agtgtgggaa ctgacggtac tgatggacca
    19621 actgatgctg caggtgcact ggccgatgga acaacagtta aaagagcaca aaaacttgga
    19681 ttgaatatag aggagtatat caataatagt gattcgtacc acttttttga aaagctgggt
    19741 gatttaatta aaacaggtcc caccaatacc aatgtgatgg atttaagaat tattattgta
    19801 gtataaaaaa ttattgatta attaaaaaaa tacttgctaa acaactggct ggatagtaca
    19861 attaatttca atatagctaa ttgtatatgc agggatgcta aaaaatagta tatgttaatt
    19921 taaaattaaa ggagtaatac aatgaaaaag aaaagcatac tttttgtttt ggcaattgct
    19981 ctggttcttt cactgcagtt tacacctatg gtaaaggcac agcaggcaca accagctgaa
    20041 aaacaacagt ttacagaagc aacacggcct gaagaaaatt atttagcacg ttttcatgca
    20101 ctggagcggg ttgaattttt aaagaaatca aatttagaca aaatatatat gttgaaggtt
    20161 ataataacca actataaaga tcagggctgg gataaagatt accaggcaat atatgaaggg
    20221 tataaaaaag gcatggatta ttactataag cgcgatatta tatatgccca ggtggaactt
    20281 gaaaaaaacc gtaaggatat ttttgaatta tataaaaaaa tagcagaagt ttataaacag
    20341 agaacacagg cattactcaa tgaatgtgcc actaaaatcc ttgacctgga tctggatgag
    20401 cgaagcagat caaatcctga caggaacaaa gtcttattca aaaatgttgc gcgtttgcgc
    20461 atagcgtatg gccaatttga tgatgggcag aatatgatgg atgaccatgt atatgataca
    20521 gcggttaaac acttcagagt tgcaaaatcg tatgcaattc agatacttga agacatagac
    20581 ccggaaaatg caaaaggcaa atatgatctg gataaagctg acaatttaaa cagaatattg
    20641 aatccgccac agcagtcggc taaataagta ttattgtaag tattatggta ttttgctaaa
    20701 aggctgtctg taataaggca gcctttttca taatactatc tttttttaag agagtggtac
    20761 actatataat ggcaaacgtt gattgcttac aaaaaatagt agtatttctt gtggtaatta
    20821 ctattactat cttttcattt aaaattccca taaccagatt ggaattcccg ctatatcgtg
    20881 ttgtgcttga tcctggacat ggtggcaagg caaccatacc aaaggatgag tacggtgata
    20941 ggtttgatgt gctttccatg aaatacctgg atgtatatcg tgaaggagca tcatataagg
    21001 attatcatga gcatatttat acgtatgaaa tcgctaaacg cgttgatgca ctattacagt
    21061 tactatcgcc caatggtaat tttgaaaaat tttatacaat actgcaaaaa tatactgata
    21121 agcctgtcaa aaggatatat atacagacat tcatgagcag gggcccatca ttaaattcac
    21181 accttatcca taaagaacct aacgcaccat acaggctgtt tgactttatt agcaacgatg
    21241 gcacgcttaa agaaggcaga ataagttata ttaacagtat taagccacat ctggtggttt
    21301 caatacattt tgctttgaat tcgtcaccat actttcgtgg tatgaatgca gtcattgcag
    21361 caccatacag tattttatat aagggattac agtattttca gggcactctt actgacaggt
    21421 ccttttttta tcacagtaag tatgctgact ggtttactga agatgaacgt aagtcaggat
    21481 ttttctggta ttccaatgat gttgttatgt atttcacagg ctatcggata aagaatgatt
    21541 atagcattaa cactgatgca ttcagaggct acaggtataa tatggttcag tgggctttta
    21601 gtgacccacc tggctgggca catattgcaa agcatcatcc gcccaaaaca ccgtatgcaa
    21661 acaatataca acagtttatt cctcaaaatg ccttttttac ccgtgaacaa agcaaatatg
    21721 aacagtatcg ccgtgacgga ggatttgaag gttacggtgg tgacaacctc tatgcatcaa
    21781 atgagattat acgatttgta ctgtataatc tttattcaaa gggcatacgg cataaagatc
    21841 agcggcttgc tccaccgtat atatcaatat ggtcagtacc actgcatatc aatgccatta
    21901 atgcatttat tgagtttgga tatttagccc gtccatatac ccgcactatt atcaataatc
    21961 atctggatga tgttgcggag ggaattgctg ttggaatata ttcacttttt gctggtgtag
    22021 aagttaccaa aaagtatccc tacacaccgt atggaaaaaa gattgacctt gaaaagtata
    22081 aaattgataa cgataatgac tattttacaa tagtgcgata gctcatagta gtatgtcttc
    22141 tggagagaaa gtgtgaaaaa actgtatgca attatcatac tatttttttc tttatcatct
    22201 gcttacgctc aggataccgt cagtgaaaaa tttgttgctg ctgctcagaa agcaagccca
    22261 tcggtagtga gtatacaggt ttttgttgtt gaaaaaggac attatcgaaa agttggttat
    22321 gcatcaggca ctatcctttc atcaaaaggg tatgttgtta ccaattatca tgttgtttca
    22381 aaaggtactg tatatcagat taatttagcg gatggcacag aatgtgaggt tgaatcattt
    22441 tctgatggaa gtatctacag agtagatgaa aaaactgata ttgcactgtt aaaggtacgg
    22501 catcctgccg gcattccttt acaggcaata gagtttgaag atgaaaaagt atatcctggt
    22561 caatgggtac ttgcaatagg taatcctttt gggctgcagc aatcggtaac aggtggtatt
    22621 gtatcgtcag tggggcgcag tgacataggc tttgctgata tagaagacta tattcagact
    22681 gatgtcccta ttaatccagg taattcaggt gggccacttg tgtcactcaa cggaaggctt
    22741 gttggcatca atacagcaat acggacgcag acgggtgggt atcaggggat aagttttgca
    22801 attcctgctt ctattgttaa acaggttgtt ggtgaactca tacagtatgg cagggtgcgc
    22861 cgtggctggc ttggattttt agttcaggaa aaatttataa aagatggcgc acaaacgcag
    22921 gttgaagtta tatcggttgt tgatggttcg cctgcttccc gtgcaggtat agaagttggt
    22981 gatattgtac gcagggttga tggtgtacag ataaaaaaaa tttcagacct ggtaaaatca
    23041 attcatgcta aacaggtggg ctcacctatt gaactggtta ttgctcgtga cggacagcta
    23101 ttgacatata attttgtgtt acatgaaaaa acaactgcta aaaaaggtaa tcaatggctc
    23161 actgctttga agtccaggta tggaatcaca attgatgatg cacgcaatgg tgtggttatt
    23221 gtcgaagttc ttcaatttgg tcttgcttac aatgtattaa aaaagggcga tatcatacag
    23281 tctattgatg gtatgggtat tgcatcttta caggatgtaa ttgcagtatt gaaaaaaaat
    23341 aatggagtaa tccaaaattg tattgtactg cgaggcggca gggttatacg atgttccttt
    23401 aattcacagg aagttgagta atcatgaagt ggatatatag taccatttta tttttattat
    23461 gtgcgatagt aactgtccat gtaactgctg atgaagcaaa agaagttgta ctggacactc
    23521 aatggtatac ttctttacaa aacagtgatg caatcaaacg attacagatg cttgacgcat
    23581 tgaaggataa agtggttaaa gctcagggcc aggttatcaa agtagaagaa tctaatcttt
    23641 taaaaaagaa atatagaatt atcctgtctg tcaatcttcc aaagatnnnn nnnnnnnnnn
    23701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23761 nnnnnnnnnn nnnnnnnnnn nnnnnngctt ccctgtcagt aagctcaaga atagtaccct
    23821 ggtgccattg tatatcctca gaagaaaagc tttttgtggt attaattgaa aatgtaaaga
    23881 aggtaaaggg aatatttact ttagctctga gacactgacg tgtggggtta tagacaacgg
    23941 aatcggtatg tttaagtaaa atgatgggaa atggtgaaga aattttaccc agcactgtag
    24001 tggtgaaatc atacgaacca tcaggatatg gccatatata acattccaca gtttcatttt
    24061 ccgggatata tgtaaaaaaa tcctcaggtg atttacatat aaggaataag tcattatttt
    24121 caattaatgt aagcaatgta tatccatacc cttttttata cacccggata ggggtattgg
    24181 ttttaatgtc atttgttctg gtaatttttt tcatgataca attttctcca gaaagtcagt
    24241 caatacatgg tgtcattaca ggcaattttt gcctaaaaaa ccagtgaaat aatataaaat
    24301 aaagcatttc aagtcaaggg caaattgtta acgtcaggta aaattattat aattaatgta
    24361 ttcatccatt taatagaacc ctggagggat taaatttagg ataccattgt ggtgaattca
    24421 aaaaattagt ttttaaaaat ttacagttcc cataaaaaag tattgaaaaa aagttgttgc
    24481 aaattgtatt atgtgcaaaa atagtggtag tccttctgct gcacgcgtat gcagtgcagc
    24541 tactattatc catagggggt aacaatgaat aaatacgaat taatagtcat attgcggcct
    24601 tcggataagc tagaatcgct taaagaaaag gtccgtgaaa ttttaaagaa aaatcaggtt
    24661 gttattgaat ctgatgaacc atggggtata cggagtttag cctatactat cgacagggca
    24721 aatgacggtt actatgaata ttttattgcc cagataaatc ccacagcggt gagcaatatt
    24781 attgccgaat tcagattgca gccggatatc ctccgatatc tttttgttaa acttccaaca
    24841 ctaagcaaaa cagcgtgacg tgtatggccg gtgatttaaa caaggttata ctggtaggaa
    24901 gagttgtcag ggatcctgag ttgcgataca ccccgggtgg gtcagctgtt gtttcctttt
    24961 cagtggccaa taacaggatt tatgtaaaga ataacgaaaa gcaggaagtt gtttcgttct
    25021 ttaattgcgt tgcatggggc aagctggctg aaactgttgc caattattgt aaaaaggggc
    25081 agcaactggc attggaagga agactgcagc agcgttcctg gcaggataaa gaaggaaaca
    25141 aacgttcaac ggtggaaata gttgctgaat cgttacagtt tttgggtggc aggcagcagt
    25201 ctgattcaca ggttactatc gaacaagatg agcccgttga tataaatagc gatcagccat
    25261 ttgctgatga tgagatacca ttctaactat tcacattaag gtataaggag tactataatg
    25321 agtgaagaaa ttaaacatga tgaaactata gaggcgcagg acaatgttaa tcctcaggaa
    25381 gttaagccgg aagcggtagc tcctgaagaa gatgataagg tgctggcaac tgctgttcag
    25441 gtgcagcgaa tacctaaatt taagaaaaaa gtatgtcgtt tctgcgaaga tccaaactta
    25501 gctattgatt acaaaaatcc ggatttgctg gtcaggttta ttactgatag aggtaagata
    25561 ctcccacggc ggattacggg aacctgtgca aagcatcagc gccatttggc acgtgctatt
    25621 aagcgtgctc gtattatcgc attgttgccg tttgttgtaa aataattttt taacgtgaca
    25681 ttgtggaact gctgcttcat tttatacttg gcatagcttt aagcgtagtt atagctatgc
    25741 tggtgtacaa actgcaatca caattgggtg cgatagctct tatcattatt gcaacagcca
    25801 gctatatagg tctatcgtac cttgtaaatg aaatgatcgg tatacaattt attattgccg
    25861 gtgcaatttt tgggtataca gcacaacggt tttcatttca gaaatatttt atgacaagtg
    25921 tgctggtatt gtggttttcg cttcttgttg tctactattt gcaatattat cttaaaggtg
    25981 ttgatgtagt tgcagacaac cacgcaatga tacagcagat gattgaagga tcatcgttag
    26041 gtgctgcaga aaagtcagtt atgatgcagc aacttgaaaa ctttatgcca acaatgcgca
    26101 tacttatgcc attttcgtat tttttcagtt caattgttat ttcgcttttt atatatgttc
    26161 ttgtgtatgc cttttttata cggtttaaat ttttaacaaa ttctaacagc acgtttgctt
    26221 tatttcgtat taatgagtgg actgtttttt tgcttattgc aagctgggca acagtgcttt
    26281 taagcagcag tgatcatata gtatgggcaa tagctcttaa tgtgggtctt attttgtgtg
    26341 tggtgtacac cattcaggga ttgtccatcg taagtttcta ttttgagaaa aagcaatggc
    26401 ctaaacttta cggtgtagtg ctgttatttg ttggcatact attggggttt caggtgctct
    26461 tttttatcat aacgatgctt atgggtttgg gcatcattga tttctggatg aattttagaa
    26521 agattgttta atttttttca aaggagtttg actatgaagg tcatattaca gaaggatatt
    26581 tcaaatttag gtgaagcagg tgatgtaaaa gaggttgcag aaggatatgc acgcaactac
    26641 cttttgccca agaaacttgt catcccttat aatgaatcaa gtgcaaaagc aatagaacat
    26701 cagaagaagc ttgtaaaaat aaagaaggaa aaacgcaaaa aagaagggca gaaacttgta
    26761 gaagcattat ccaatgttgc cctgacaatt ggtgcacagg ttggcgaaga ggataagctg
    26821 tttggttcag ttactgcaat tgatatagca caaaagctat cgcacatggg atactcaatt
    26881 gataaacgaa aaatagtatt gcctgagcca ataaagaagg caggggaaca tgaagtaaag
    26941 attaaattgg atgaagggct gtctgcaacc attaaggtta ctgtggtgaa ggagtaaccg
    27001 catgcttgat gctggcatgc cacctcatga tgttgaggcc gaagctgcgt gcttatcttc
    27061 catactatta agcaaagatg ctttattaaa ggttattgaa atcctccagg tggaggattt
    27121 ctacattgat gcccaccgaa tgatttatga ggctatcagg gaattagaaa taaaagccat
    27181 acctattgac cttattacaa taaagcagcg gctgattgac aaaaagcagt ttgagaaggc
    27241 tggtggcgac cctgcactgg ttaatatata ccgtacggtt tctactgcag ccaatgctga
    27301 gttttatgcc aacagggtta aagaattaag cctgcggcga aagctcatag aaacctgtac
    27361 caccatagct gaaaaatcat acgataccac cctggacaca aaaatacttc ttgatgatgc
    27421 tgaaaaggat atattcctca ttacgcagcg ccgcataaca agtgattttg aaagcctggc
    27481 aagtgtgttg aagcagacac ttgaagacat aggccacatg tatgaaacaa aaaggcctgt
    27541 tactggtatt gcaagcggat ttaccgatct ggatgccatg cttaccggtt ttcatgaatc
    27601 agagcttatt atcattgctg cccgtccatc aatggggaaa acggcgcttg cattgaatat
    27661 tgccaatcat gtagctttgc gtgaaaagca gccaacattg ttcttctccc ttgaaatgcc
    27721 tgcaacacag cttgcaaccc gaatattgtg tattgaatca atgattgata tgcagcatgt
    27781 acgaactggc cagataagca gtgaacagtt gcgaaagctc attgatgtgt ccggaaggct
    27841 ggaaaagtcc cccatgttca tcgatgacac accaggatta agtatttttg aactcagggc
    27901 aaaggcacgg cgttttgccc agaaacagaa ggttggactg atagttgttg attatctcca
    27961 gctcattgtg tccaataaca gactggaacg ccatcagcag atagctgaaa tcagtaatgc
    28021 cttgaagcaa cttgcccggg aactatcggt tccggttatt gcactgtcac agctatcacg
    28081 tgctgtggaa tcccgtactg accagaaacc acagctttct gacctgcgtg aatcaggagc
    28141 aattgaacag gacgctgacg ttgtgttatt tatataccgt gaggaaaaag ttaaaaaaga
    28201 aactgaacgt ataggtatag ctgatatcat tgtggcaaaa cagcgtaatg gtccaaccgg
    28261 aacaattgag ttacgcttct gggacaagta tatgaagttt gggaatgttg aaaaatttcg
    28321 tgaagactga tatgaatggc actgaaataa caataccacg aacggttgtt caattttttg
    28381 aatcagtagg caagcattca ctgtttttta cccaaactgt aaaatggatt tttaagccgc
    28441 catttgatgc aaaaaatatt ctaacacaga tgcttgaaat agggtacaag tccttgccgg
    28501 taacctgtat cacgctcttt ttcaccggca tggttatggc attgcagata ggcaaagcca
    28561 tggattccct tatgtcagga tcatcagtat ttattggaag cgcagtatca attgcaatgt
    28621 ttagagaatt gtgtccggtg cttaccgctt tgcttttatc aggaagggtt ggttcagcca
    28681 tagctgctga aattggcacc atgcgtgtca cagagcagat tgatgcgctc atcacacttg
    28741 atgcaaatcc aattcagtat ctgtcggtac cacgtttttt agcctgcctg gttatgacgc
    28801 cagtacttgt tgttgttaca gatgttgttg gttcattagg tggggcattc atagcatact
    28861 ttaatttagg gataactatc gacaaatatg ttgaaaatgt ttttaactat ttaggtgtca
    28921 tggacttttt gacagggctt tttaaatctg ttttttttgg cattgaaatt gccattattg
    28981 cctgctatca ggggtttaat acatctggtg gggctgaggg tgttggcagg gctacaatac
    29041 gggctgttgt tgcgtcatcc atgactatac ttatttcaga ctattttctt acttatcttt
    29101 tgcaatttat tgagtagtgg aagtgcaggg tttatgaaat cgctgaaaaa aagaaaagag
    29161 gaatttatca agtttttgca aaaaaataaa attccaaaaa aggtgctgga tgcatttgta
    29221 gcagtcaatc agcaggattt ttttgatgaa atttatgaag acaggttata tgggcaactg
    29281 ccaattccaa taggcagagg cgaaaccagc gatgaaccgc ttgcacttgc ccgtatgata
    29341 cagtattgcg gcattaagaa agatttccat gttcttgaga ttggtactgg ttcaggctat
    29401 tcaactgcag tgttgtcaca gcttgcaaaa tcagtaacca ccattgaaat agatgaagag
    29461 cttgctgttc aggcaaaaca acgcttcaaa aaaacaggat tgtcaaatat tcgtattttc
    29521 cagggcgatg gtacagatat tgatgaatca ttaggttttt ttgatgcgat aatagtgctg
    29581 gcaggttgta aaaaacggcc attgctatta ctccatgttg ccaaacctga tagcatgatc
    29641 gtattcccca tgggtcctgc ccatcagcag cagatagcac tgatgcttaa tcaccgggat
    29701 gataatgttg aggggttgta tccagttgtt ttcaaggaat tctgcagttt catgcccatc
    29761 attggaatat atggctaagt acgataatga ctatataata atgtgctttg aaaacagtaa
    29821 tgcttatata tctgatttct tttttttatc tggatcttcc tgtggcttat aacaatatcg
    29881 acatccatat attaatatct ttgtgctatc ctttacaatc tttgaggcat ataatgcttc
    29941 atacttgctg agttttgatc cgcatactgg gcatgtcctt tcttttaaat gtacgggttc
    30001 attaacgggt tgtgatttga attgtatttt gtcctgcgtt actttttgtg caggttgctt
    30061 gttgtcaggt tttggacgtt ctttaatatg atgatacagg gaaagcagat ataaaaacac
    30121 tgcaatgcct gcaccaaatg aaagaatata aagaatagtt tcaatcatcg ttgtgctcgt
    30181 tttgtaataa aattaaccat aattgtttct tcatggggtg gcttaagcgt ataatcccca
    30241 acaacatcaa ctactgtaag accgcattgt tcaataagag gtagtacttc atcaattgta
    30301 tataatcgct gaagatgttc ttctaccagt gtattggtat gcctggtaaa tgtcaacttt
    30361 gagtataata tttttgaatg tttatcatat tcattgtacc actgaatatc ggtgccttta
    30421 acttgaaatg ttaatagctt attgtgaaaa ttctttttaa tattatactc tgtggtaaga
    30481 tcaaataaaa agatactgtc attgttcatg cagctatgca cacagtgtaa tgcgctgaca
    30541 aaatcatcat aatccaggat atagttaaat gtatcgtgaa cagaaaaaat aaaatcaatt
    30601 gtttttgaca gagcaaactg tgtaatgtcg ccgcaaaata cgtgatagtg tttatatgct
    30661 cgcgcttttg ctatttttaa catttcaagt gatttatcca tgccatatat ttcataatca
    30721 tccatggaga actttggacc aaatttgcca gtaccacatc ccaactccag cactgttcgt
    30781 ggattattaa tatacctgca cattatcatt cgcagataat gataccaggt ggcataatca
    30841 acatgtttca gcatatagtc ataataggga gcaaggtaat ggtatggttt cattgtattt
    30901 ttcggttcaa tcgattaatt ctgtgggtga aacgctcagt gtagtgttca atgagcttct
    30961 ggttatctga aagctcttta ctatgagctg tcgcccaagt gcagtaactc agtgctttat
    31021 gataatcttt aacacgatgt tcatagtgtt ttgctaactc ctcaagtgaa aacagattta
    31081 tatgcttttc ccagaatgta agggcctgct ccagaaggcc agagcgcttt aatgcagttg
    31141 aaaacttttt aaaaagggaa tggtcgttgt cgagcaatgc atagccagca tactcaatga
    31201 gttcaatgaa cagttgaata tttcgggaat ataggtgctt tgcaatattt ttatacaggt
    31261 gtgtgggtac ctgatcaaac tgctttgtgt gtataagcgt gtagagggaa ttgaaaaaaa
    31321 caagtagcag gaccatggat gtaatatcaa tacgattgtg ttcaattacg gcagtaaggc
    31381 gcgggtcata acctgtttgt tgataggtaa agtacacatc aggaatctgg tagcctggta
    31441 tatcgtcggt cctggtaatc ccaagtagaa gctcttcaag tgattttaat gaacagcttg
    31501 catagattga tttaaaaatt gagcgtgcgg gtttaagtaa atcaatgacg ggcatagaaa
    31561 cagggaagcc ctgaatgcgg ttaatcctgt agcgcgtttt tataatgggg atgtcaaagg
    31621 ttttgccatt gaaggttaca attgttttaa atttttgtaa ccgttcagca cagtgaaaaa
    31681 gtactgctgg ctcttcacca tactgacgca tgaaaatctg ctcaactgtg aatttgtttt
    31741 cactaaaata ccctaagcca attaaaaatg cataattacc tgttccgctg gacagtgatg
    31801 ttgtttctat atcaaaaaaa acacactggt caagtgtgac aggttccata cgatgcattg
    31861 cacagtaggc tgcaatgtca gtgacggtat catcgatatg taatgatgca actggatatt
    31921 gctgcacaaa cgtaatggtt tggccaaacg gtgtggtaat aatttcccca cctgcgtcct
    31981 gtatccatga actatcggtt acaggcttgc ctgagtcagt gtagtatatg ttgagtttgt
    32041 ccttcagatt catggtgtaa gtttttaagt aatgctatta ctttttcttt aatgccttct
    32101 ctggtgatca tggggccaat acaggcaggg cagccatgag tacagctgca agcggctatt
    32161 gcctcctgtg atgcctgtgc tatcagtcca aatatgtgat atactctttc ggatattcct
    32221 acaccgccag ggatggcatc atatacaaag attgttggct gattggtgaa gtgtgaacgg
    32281 ttttcaaata caacgcgtat atcggcataa tcagatagtg tatacagtgg cgcgatgttg
    32341 cgtaaaatat atgaaagacc taagaatacc tcaccagctt cttcgcgttc catagtatta
    32401 ataatttcat cgctgaagct acaccacatt gctgttgaat gcatttccag ttcaggaagg
    32461 ttaatctttc cccagccaac attttcgtgg gttccaaatt ttatcttttt aaacatgata
    32521 gctttagtgc gtacgttaac ctcgcccagg cttaacgttg caattgacag cggttttgat
    32581 tcaaattctt caagcacatg gatatcggtt ttttcttcgg catcggtgta gtaatcggat
    32641 tctacttcat ggcaataggc aatcctgcgt tcccagtcta atttgtcaat atagtactgc
    32701 tgaccctgat gaagatagat agcatcatcg tgtatcatgg ttggcgctga aggaaggtct
    32761 atttcgccaa taacctgtgc atgattgtct ttatgaacta tcaccacatt atcatgtgaa
    32821 gcgctgcgca atgatatatc atttgcaggg tagatatcac tcatccagtg aaacttgcca
    32881 gctgattctt ttaagatatt cttttgcgcc aggtattcaa gcatctcacg ggtggtgcta
    32941 tggtgtggtg taaacagttc atcaatacca aagggaattt caaatgctgc acatttaatg
    33001 tggtccataa gaatgagcag gttatcagga ttaatgtttg ccatctcagg attgttttca
    33061 aacacaaagt gtggattggt tgcgatatac tgatcaagcg gtgagcttgt ggcaaccata
    33121 attgcaaggg aatcggttcc ccgcctgcca gcacgacctg actgctgctg cagtgaagct
    33181 attgagccag gataaccaac agagatggca caatccagca taccaatgtc aatacctaac
    33241 tcaagtgcgt tggttgagac aaccgcgttt atcttttttt cacgtaaacc cttttcaata
    33301 gatctacgtt cattgggtaa atatccgcca cgatatgcgg caactgaaaa ccctttacac
    33361 tgttctttta aatatgttgc gatgatctca acgcgcaatc tgcttcgaca aaaaactatt
    33421 gttgaaatgt tattcttcac gcacagtgtg ccaacctttg cagcttcctt cacggctgaa
    33481 gcccgtatac ccaaagcctt gtcaaccaca ggtggattat atattacaaa atgcttttta
    33541 ccgcagggtg caccgttatt gtcgattaca gtaaatggca tttcaaaaag cttttcagca
    33601 agttcttttg gattggcgat agttgctgag cagcagataa attgcgggtg gctgccataa
    33661 aatgagcata tacgtttaag tcttcgcatg agattggcaa aatggctgcc aaagactcct
    33721 ttataggtat gaatttcatc aacaacgacg tactgcaaat tttcaaaaag cttaacccat
    33781 atagtatgat gtggcagaat acctgaatgc agcatatcgg gattggtgat gacaatatta
    33841 cctgctgtgc gaatagcttt acgagctgac tgtggtgtat caccgtcaaa ggtgtatgtt
    33901 ttaatggata aaccggtgat cttcataatc tcttgaagtt cagaaagctg atcctgcgat
    33961 agggcttttg tggggaatag gtacagcgcc cgtgcatcgg gatgtttggc cataaaatta
    34021 agcacgggaa gattgtagca tagcgttttg cccgaagctg ttggcgttac taccacaata
    34081 ttttcttttt tttgaactgc atcatatgct gagcgctgat ggctgtaaag attttctatg
    34141 ccacgctgca tgtatacatt gcggattcgt tcgtctatgt catccggata tggtgcatag
    34201 acagcttgcc tggcaggtac cgtataccat gctgcaacct gtttcataaa cggttcatag
    34261 tttttaagat attcaataag ctgttcaata gtcattggat taaagcactc acacgatatt
    34321 ttgtcactat catatcgggc tgatatgaac gctttgcaaa gcgcagcggt tctaatccta
    34381 aatcctgttc aaagttaaaa tatttataat ttgcgggcaa tgcttttgca cagtgattat
    34441 acatgtattg ataaattcct ttgtaacttg ttatagcttt tgcaaaatga attgcaaaca
    34501 tatcatcagt tattgcttct ccaatgatga atgcagcagg ttttccttca acgtagtaca
    34561 tgctgccgca cagatttaat tcgtcaagca acatacacgc ttcaatgcac gcagcaatat
    34621 ccgcatcatt gccggattcc tttttccata tgtcaagtat atataatgca tcattaactc
    34681 tctggctggt taatggaagc acttctacag aatattttgc cgtaaactga tacagaagat
    34741 ttctttttcc atgaagtttt ttcccggaat aggtggctaa cttttctttg gtatagacat
    34801 aatcggagtc gccatcattg taatgatagg taaagccttt ttcagtaaaa aaatgcaacc
    34861 agtcatggtg aatgggaaat agacacatat tgggttgcag gagttgtacc agtttttcag
    34921 catattcttc atccagcggc ataacaggca taatatagtg tataccgtca tatgttacac
    34981 ctttaattgc tgtgatatcc cctgttataa ggatgtactg gtgaactgaa cgaaaaagat
    35041 acaggtttgc aaatgaatac tcagatatag gtatggttac cttttgcaaa agaggctgaa
    35101 gtaataattt gtgcgatagt tctaatgatt gttc
//
LOCUS       DAORYL010000022        34985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_991_length_34985_cov_13.126968, whole genome shotgun sequence.
ACCESSION   DAORYL010000022 DAORYL010000000
VERSION     DAORYL010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 34985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34985
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_991_length_34985_cov_13.126968"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..539
                     /locus_tag="PLV81_06045"
     CDS             192..539
                     /locus_tag="PLV81_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOM09698.1"
                     /translation="MAFKEIGKKIQIAREEKGITQIELAKILGITQAALSNYELGKRR
                     VYFHQIELIAEHLEKPISYFLDDESMLEDDENIINDEMLDLLTSKVKQLNRQEFSELD
                     QFIDFLLWRKHNE"
     gene            532..1653
                     /locus_tag="PLV81_06050"
     CDS             532..1653
                     /locus_tag="PLV81_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOM09699.1"
                     /translation="MSNILLPQDLNQSLHECIQLLDSQSSVINNLSTTERVLAYYSIY
                     CKNKLHVLLLPEEYGGLSGSFSHAGIIVETLAYNYYNTLPTLLTTIHCYELIKSFADN
                     NKKKWLLNAVNNTPKPLAFCLTEEHAGSDITSIQTTAIKNDNKIIINGKKVIVINSGI
                     ASFYLVIAQSSKKGRAGLTAFLIPETADNISFEKLEVSSFLHSVIGTVTFNNVIIDTQ
                     YQIGDEGSGFFLLTEMLDKGRPLVAACCVGAAQHALDIIIDHTKTRKQFSRYLYDFQG
                     ISFPIAEFETRLHAARLLYNDACEKIDNNEPFTKEASMAKYYASRLFLDITNFGMDTL
                     GYRSIIDASEMKLLNDFAKTMMFIDGTENVQKMIIASLL"
     gene            complement(1670..2851)
                     /locus_tag="PLV81_06055"
     CDS             complement(1670..2851)
                     /locus_tag="PLV81_06055"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOM09700.1"
                     /translation="MKKIYLILVLVITCSYCSKIHSKLNDTVTITFIGDVIMHGPVKS
                     FAFRNNINDPKTARSINNGGFDILFEHIKHEFESSDYVVANMEFPVAPPYTSKAFIFN
                     CPPYVLDAFKNININIVSIANNHILDQGYDGLISTIQILESKHIQYMGVSKNNSTLNG
                     IIVGNNPVKVGIIAYTGVLNYGLSKKYKNNIHINDFYDTKKVLTDIAEIKNKCDFLIM
                     IAHIGNEYSTQPSKKDMNIIKTYCEAGVDCVIGHHPHVIQPVEQIKASDGRICTVFYS
                     LGNFIANQSSTHNDPKSGVECSTREGLIVSLILGKDQSGIYHKFNILPIMIYNVPDTE
                     SKYKYGRRIQPIAIKQYVYKKQENNNANSEEIKILNNRIKEIKKHLFLYGQYDNVVYL
                     E"
     gene            complement(3139..3612)
                     /locus_tag="PLV81_06060"
     CDS             complement(3139..3612)
                     /locus_tag="PLV81_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HOM09701.1"
                     /translation="MNFVIGSTRAGLQLKRAIIDYLVKQGHQIDDVGMKDEANFVPYH
                     IAAANVASLVSQGKYEKGIVICGTGAGSAIVANKFKGVYAVHCTNSFEAKKASAINNA
                     NVLVLAEWLTPANHAFEIINDWLSTPFGSGFEEEWQKFLANCIDEIKDMENKNFK"
     gene            complement(3626..4570)
                     /locus_tag="PLV81_06065"
     CDS             complement(3626..4570)
                     /locus_tag="PLV81_06065"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOM09702.1"
                     /translation="MSYHDMEMRAVYGLTLCELMEHDKNVLCLEADLSKASGTNPAVC
                     TAFPENFINVGVAEANMIGLAAGLAREGKIPFCASFTCFASRRVYDQITISVAYANTN
                     VKIVGTAPGITQGPNGGTHMDFLDLAIMRAMPNMHVYSPCDVYELRAVMKYMAAHHQP
                     TYMQLLRPKMPVVFNESFTFDPNKATKISEGSDVTIVTTGYTTHLAINAVEQLKKEGI
                     KAEHLHYSSIKPFDEDLLIQSAKKTGCVVTVENQNIIGGLGGAVCEVLSEKLPVIVKR
                     LGIPDAFGEVATEEYLLNKHGFNVEHIITACKDMIKSK"
     gene            complement(4686..5528)
                     /locus_tag="PLV81_06070"
     CDS             complement(4686..5528)
                     /locus_tag="PLV81_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOM09703.1"
                     /translation="MNTMSTEQLEQRAKEFRYVITDMICRAGSGHLGGSLSLVEIVTT
                     LYYRIMNIKPENPAWEDRDRLVLSKGHAGPVLYVVLAYLGFFPKSWLKTLNQNGTNLP
                     SHVDHNKTPGIDMTAGSLGQGLSCACGMAYAAKLNKKSYNVFCIIGDGESNEGQIWEA
                     AMFAGHNKLDNLIVICDYNKMQIDGKTDEVVTLEPLADKWRAFNFEVFEMDGHNWDDI
                     YSTINKAISVKGKPAMIIAHTIKGKGNAEVENKVASHNIKINNQSDYDKYMKGLGYTM
                     TLPY"
     gene            complement(5597..7042)
                     /locus_tag="PLV81_06075"
     CDS             complement(5597..7042)
                     /locus_tag="PLV81_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOM09704.1"
                     /translation="MKLSHKKTLYLFLAIGIALAIGLLPIEYWQNTGDKSIQLTTQGK
                     YSLAVLAFAVIGWVTEIMPFAITGLFSLSLLVITGCANLADLVKDGFGNQVIVFFIGV
                     MIIGAIVNETSFVSIITQKLYKRYCSQPKTLIFIILAIGMILAGFVTEMAAAAMVLPF
                     AVSILKRAHIEPLKDNYGKAMMIACGWGPVIGGISTPAGSGANPLTVGFLKDLAHIDL
                     SFAQWMGIGFPAALLMLPFAWYILVKTFNIESVSLHVDTEHNEKIQIPLHDIIIGILF
                     ILTILLWIVEPVLHNLFPSLQYITMSFVAITIACCMFLPPFAQLSWHKVEHAINWGAI
                     ILIVSGLALGTTIYKTGAAKWLAWVLFKNLNLLHPVIMVFAVVIGVSIVKVMFSSNTV
                     TGIIMVPLLIALSGTIGIDPALVAIPAGLTSSLSFILVTSTPTIVIPYTAGYFTIADM
                     VKPGIYMTIVSSICVTISFLIFGSLFNIITW"
     gene            complement(7109..8113)
                     /locus_tag="PLV81_06080"
     CDS             complement(7109..8113)
                     /locus_tag="PLV81_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOM09705.1"
                     /translation="MKLSYKISPINDTIFRISLPLPGYPPYINVYLFKGEVNTLFDTG
                     IFWTWRLLRRALHDLGLQFSDIHQVIPSHGHVDHYGAAYAIAKRNKHVEVITHIADAK
                     RIEEGLYNKPSIMLKFIESMGVPKPLIVPLAFLDLTFKSMGHPVIVTRKITDDQCSLQ
                     VGNYNATLILTPGHSKGAMCLYIEKEGFLFSGDHIVPGVKPIILAGFEPNTSFPVAQS
                     HTIFHTSLKKIEKLSPQYIFPAHGEPFQEKLNRLIVKYQKVYQFRKKRMYNLLLNNDY
                     TVYGLARQVFKNLKGINLVLDLYLSLSETYTHLEAMCEEGDVIKYNFDNTYYYTATLL
                     "
     gene            complement(8418..10403)
                     /locus_tag="PLV81_06085"
     CDS             complement(8418..10403)
                     /locus_tag="PLV81_06085"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015614294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HOM09706.1"
                     /translation="MLKIEHNDSSFSLYYKNRCILMHSVKKPCIAVGFGNAVYSMKEG
                     NFKLKDSITSKQYLKHFTVKEDKNNITIQLQDAIELTITADTYCTINIKAKKPFNRFW
                     LHCVADIHEYIYGCGEQFSKLDLKKKNLPLWVQEQGVGRGHDLITLLANVLYGAGGAW
                     YTTYYPQPTFVSSSNYFIHCNASSYAEFEFKKKEHILHFWQIPESVVVGVYEKPAECI
                     KAVGDLLGRQPRLPQWVHDGMWIGMQGGTDVVKKKLQVALDAGVKVSALWVQDWVGRR
                     ITWFGKRLFWNWKYNQELYPGLPSYIQELSHKNIKFLGYINCFLNSEGDLYKEAISKG
                     YLVHNQDGTVKTFDTTGIIAGTLDLTNPQTREWIKNVIKEHMIGIGLGGWMADFGEYL
                     ETDAKLYSGESAELYHNKYPADWARVNYEALEEAGKLGEVVFFTRAGYTGTSKYSTLM
                     WAGDQLVNWSMHDGLASVIPAGISLGFCGVGYFHADIGGYTTLGWIKRKKELFMRWAE
                     LGAFTAVMRTHEGNRPDVNLQFNSDMETLQHLAKMTKVHTILKPYFEHCKDEYHTDGL
                     PLMRHPYIHYPDDEKLHSLQYQFLLGRDVLVAPVYRPRRKKWNVYLPKDEWVHVWSGK
                     EYSGGWVKVEAPLGQPPVFYRKKSQFKTIFEAIKDIK"
     gene            complement(10397..11713)
                     /locus_tag="PLV81_06090"
     CDS             complement(10397..11713)
                     /locus_tag="PLV81_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HOM09707.1"
                     /translation="MKEVTVGVVCFARKTFDYSAAKEIYGSIQSQLKTIKAVQWHCYN
                     ELVFEVEDAQKAIAFLKKNNIDALVCISGTFHLGHLALELIKHVSVPVMLWGLPELPY
                     DGGKIRLNSICGVNLDASNLYKAGIRNYYAHFGNTIDTDWIGAIRIISMLKNAKIGLV
                     GYHAHGFFNLDTWHPALYAQCGTMIEHYELPHVFNYPVNKQYQNQYADDIESNFDTGD
                     INSTQKQKVAELSSRLHSFMEDNNLTALAIRCWPEFAAEFGIAPCAAMSLVQAQNFVI
                     ACEGDVDGALSMLAHKALGAQTPFLFDFSQVNLEENFALLWHCGVAPFTLKDTASTAS
                     LDTYFAAGKGVTAGFVLKPGDVSICRFDFDGSNYRLLLLKAHGVTMDKLLKGTYLKVT
                     FDVPVRNVLDTIIYNGIAHHASMVYGDFIKPFEIAARIQHWGIVTC"
     gene            complement(11723..13162)
                     /locus_tag="PLV81_06095"
     CDS             complement(11723..13162)
                     /locus_tag="PLV81_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015920004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOM09708.1"
                     /translation="MNQERKITFKNLLSYGVGDIFGGGSFVVINLLFIYFLTDIAKLN
                     PALAGLVVLAGKAWDAISDPIMGYISDTTKSKYGRRRIFFLLGIFPIILSFIAMWLPF
                     SSESQIIKFIYYACAYVFFSTAFTMVMIPYSALNAEMTREYKDRTRLSGARLIFSQIS
                     SLISGTVPKMVVDAFTDKGQGFLVMSIVFAVFFALPWIIVFFGTWELPDVKVENKSLT
                     DFLKNLLVIWTNKSFRVHIGLYICAYSALDLIMAMFIYYLTYYIQKPHIFTMCLGAML
                     VTQVLFLPVYVFIANKKGKGFSYRVGVSIWAIALVSTLLFDSATPLQYIVALSVLMGA
                     GMSAGTMIPWAILPSVTDVGELITGKQQAGVYAGAMTFIRKLVQAVVLAIFGLVLQTI
                     GYKANTVQSPETLEYLRLMFALVPSTLMLCGILISLKFKITPVTHPILIQEINRLKSG
                     GSKDAVEPFVKKVCEVLTGYKYENLFKKQ"
     gene            13313..14503
                     /locus_tag="PLV81_06100"
     CDS             13313..14503
                     /locus_tag="PLV81_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOM09709.1"
                     /translation="MAHGEKINKRKENKRKHIVDIFRQHPVLSKAEAKRYSGYSMDTI
                     MTSFKILKNTGIIVPTEGKQKEKGRKALFYTLNHEKQVYLGITFNQKAIYTSIVSFSN
                     KVLAKTKVGISSSISKEEFTRVMLQQIDEVLSKNPVFTNNLVAVGCTVPGDIDTRNGI
                     LRSYTLMPQLKDFNFNELLSTKFPNTRIFFDHNISSMISYILSTSDINQRYDRIMVVS
                     ARAASAVGLIYKGAIVNSRGEIGHIKVCDDNHQCICGRSGCLDCYFSYEAFVKLLQQQ
                     NAIDENECYDNVIAKLNNHYTNRTEGIFEKLDTALKFFSEALLDVTNVFSPDYILLTG
                     DLFEIYNDPVAQIDIHIKDLFHDTGYITMYKRALMEFQSIKTESIAHGVCLSLIKQDW
                     GYSA"
     gene            complement(14504..16552)
                     /locus_tag="PLV81_06105"
     CDS             complement(14504..16552)
                     /locus_tag="PLV81_06105"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOM09710.1"
                     /translation="MIKKNNSIIILILILIIISIISQSLVVAEVLYLSKWKYATGKSE
                     QGYHDAIKMPFKPLNATNNLHTLVPDYEGFVWLRSDFTASDQIVNAPLIGVLLGRIMI
                     ADETYCNGELVGITGQFPPNFFSEWNKYRLYMLPKSILKTNENNVLLIKVYVNHEGSI
                     AGKNIIGNYKELEKEYDYLDFIDSRINAIISFLFLLVGCYYILMYSLRKKDIENLYFG
                     LTCIAFSLYLINFFVTRLPGFHYNLISYLLFQKIIFVLIFVIAYLLSRFLTRYLNISD
                     NVIIKALLIFATVVPSIILLLAKDYPTFLNIRYKVILFIIIPAIYIISITVAALKQKK
                     REAKILIIGSIPFYFCAFWDLIVHNILKIDDAIYLMGFGFPSFLISIAAILAIQTVQY
                     HNEVEELNVTLEKKVEDRTRQLYEANQELKLALNQIREQQEIAQRDMIMAVNVQRSII
                     PQKAPVVDNYDIGLYAQAMSGVSGDFYDFYIENDKLVGIGLFDVSGHGIASGLLTIMS
                     KSIIFRHFMKLINLHLGKVIEFINQELIQELEHVDNYLTGILLRFKNGKVEYVNAAHT
                     EMLLKRKESNNVIVCTHKERDIKGMFLGKSVFQTTYTPLQFSMWPGDQLLVYSDGLTE
                     QKNKDAEEFGFERLQEAFLHAQGQTAQEICDYIIKTFNEFRGDVGLGDDVTVIVLQKK
                     "
     gene            16841..17059
                     /locus_tag="PLV81_06110"
     CDS             16841..17059
                     /locus_tag="PLV81_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09711.1"
                     /translation="MPAKKKSNSFIKQLKKYISIKGLEIVIHLVNGEVIELQNNVRLE
                     KNNIVVRNKNREFHIPISDIKSIDLYAA"
     gene            17291..18127
                     /locus_tag="PLV81_06115"
     CDS             17291..18127
                     /locus_tag="PLV81_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09712.1"
                     /translation="MTRIHIQVINIISVILFSFITAQVINQSIKYFIKPADFKNYIHA
                     RYKPATTSLKSFNEYQSMLDSNFFKVATVDSSGAASISSEINELVVLGTISGPPGIAR
                     ALIKKQSESSAQIYKIGMDVYGFKLVKIENAKVFLKKDGKVEIIDMFAKDKSSSSSSG
                     PQLSQQSSPNRIVKSISKSEIQQKVLNNLDNALNGVRAGPYRVGGKIEGFKLFSVAPF
                     NILYSMGARSGDIVKRINGQPIDSTEKLMKMWETLKTDTRFVIELERSGQMITYDLNV
                     SD"
     gene            18169..20028
                     /locus_tag="PLV81_06120"
     CDS             18169..20028
                     /locus_tag="PLV81_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOM09713.1"
                     /translation="MKKNKLNFNYYLFIILSSLFIISFLYAQQENTNPEKPAETQTPQ
                     AEIITPKDEQKESQAKPEIKIKKNKGKKKSKEQYFTLNFKDVELSEFLGVMSQLIGKN
                     IVIDERVRGKITISSVKKIPVKDAYEVMKAILELKGFAVVESENLIKILPIKDAIKKN
                     VEVIIDTTKKQIVPSDDQTITYLQEIKNADAEQIANVLRALKSPNTDIIVYRDLNIII
                     YSGTASEIHGLIKIASSLDKKIQELEVDKTIKGDIHVVHLENADAEKLAAVLSRIPFA
                     EYAAISKGDQSHQVDVTKQAQQIAQAKAAITDKQKLSIIANKETNSLIITAKPEEFRM
                     IKNIIDQLDIVREQVLIEALIIEVSADSGWGFGIDWMLGDQYSSHLYGGSSIQGTPPS
                     YTLPSGMTGKKIAVPLSTGFQIGYLSDKSMLGFALLNATGAEKHYNILSTPQILTTDN
                     QEAEINVGEEIPVPTNNRISDTGVQFYTYEYKSVGVKLKLTPHITSQNSITLDLYQEV
                     NSVLGQTTVLESGSVIPPKLGKRDIKTKINIADGKTIVVGGLITNNKSVEETKVPILG
                     DIPLLGWFFKRKVVEYKKTNLLVFITPHIVTKQSKIDALTEQKREEQRRLQLD"
     gene            20031..21737
                     /gene="gspE"
                     /locus_tag="PLV81_06125"
     CDS             20031..21737
                     /gene="gspE"
                     /locus_tag="PLV81_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HOM09714.1"
                     /translation="MIGIKNKKIGQILIDKRLITNEDLQDALLSQQSTPLKIGQILIK
                     KGLINESDLLQALATQFSMEFAETLNFSDDENILSAIPAQFLKRYKIAPYSVNVKKNI
                     IKVAINDPLLIQPLDNIKIIFENYKIVPVLSTEKEIQKIINNHYELLKENSTTSVIEN
                     LEESDFEILTSPITETEDILDMANEAPIIRLVNTVIKQAIEDRASDIHIEPFEKDLVI
                     RFRIDGILYNMFTPPKKYQAAIISRIKIMANLNIAENRLPQDGRIQLKVGGKDIDIRV
                     SVFPTYYGERIVLRLLNKSDMKFDLDSLGFSKETLKVFNDLIKKTHGIILVTGPTGSG
                     KTTTLYSVLTRLNTADVNILTVEDPIEYQLHGVGQMQVKPKIDLTFAAGLRSILRQDP
                     DIIMVGEIRDLETAEIAIQAALTGHRVFSTIHTNDAASGITRLLDMGVEPFLIASSVN
                     AFLAQRLVRTICPHCKQSYKPDPVVLKDLGIKPSQIQGKKLYRGKGCDKCINTGYLGR
                     TGIYELLPVTNDIRKLIMEHADAVQIKEKAISNGMKTLLQDGVEKALQGITTLEEVLR
                     VS"
     gene            21741..22991
                     /locus_tag="PLV81_06130"
     CDS             21741..22991
                     /locus_tag="PLV81_06130"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOM09715.1"
                     /translation="MIFEYIALTNKGESVADIIDAPSEVIARQRLRANGLYIVKISKQ
                     DSEIAATPEISSNIFNTIIDRISYYISLQLSAKEISTFSRQLSTMLKAGMPLLTSIST
                     IIDQIENPIFKRAIIDIKTKVEEGSSLSNALERHHYIFNEMYINMIRVGESLGSLDNA
                     LERLADLEEKKNLLKSKVQSALWYPAFMIMFAIAVVTFLMVSIIPSLGRMFAELGKDL
                     PIPTKIVMGISNFITSFWYVIIIIIIVGIYYFNRYKKTDEGRRKIDELKLKLPVVSSL
                     YKKILIHRFTYNLGILLSNRVDVIKSFEIVQRLMRNVLIEENIKKAAKMVQEGSSISN
                     ALSKERFLPQYIIGMIAAGESSDTVDEMLLNIGKIYENEIELSINSMTSLIEPIIIVC
                     MGFVIGLIVISVLLPIFEMNLIVK"
     gene            23024..23500
                     /gene="gspG"
                     /locus_tag="PLV81_06135"
     CDS             23024..23500
                     /gene="gspG"
                     /locus_tag="PLV81_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006922378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOM09716.1"
                     /translation="MKLQSLLILIKRISKYLNSDKGFTLIELLIVITIIAILSIIVVP
                     RFMDIPQKAKVEAARQQIAAFSMALDHYYLDNGNYPTTEQGLQALIEKPTTDPIPQNY
                     NPKGYMDKKKIPNDPWGNPYVYNCPGQHGNDYEIMSYGADGKEGGSGFDSDITSWQ"
     gene            23515..24084
                     /locus_tag="PLV81_06140"
     CDS             23515..24084
                     /locus_tag="PLV81_06140"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOM09717.1"
                     /translation="MIKCLKTSNNGFTLIELIVVIAVFSILSIVVMPRIINYLSSARD
                     NFLILSTIVTKSFDDAFINNRTNFIVFHCQSKENEISEISNPLFERTNGISVAILNEN
                     SEFIDNPNKLLKNREFPDSFAIKEILLPDGSSYTSGNVMIPVYSSGSTSGAIVHIITS
                     NEEEWSLIINPYIKVPEVVKGYTTYEDIK"
     gene            24068..24445
                     /gene="gspI"
                     /locus_tag="PLV81_06145"
     CDS             24068..24445
                     /gene="gspI"
                     /locus_tag="PLV81_06145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HOM09718.1"
                     /translation="MKISNNGFTLIEILVAIAILSIAMLGIMSAVSSGIFGITKNKNL
                     TIAMLIAQSQMNKFLIDNQRGPDLQDEPVPDYPGFSFSRTITRYENAMIGPIDAYKVT
                     ITVKWLERQQERSYSISYIYPKR"
     gene            24447..25121
                     /locus_tag="PLV81_06150"
     CDS             24447..25121
                     /locus_tag="PLV81_06150"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOM09719.1"
                     /translation="MEFQDCLHVNKKGFTLIEILVATAVSSIILLMVYTIHHSIIQSV
                     NSLSKTALFYEEVNLALYRMNRDFSCAYFDKYNEKLFFIGTNEFGDKNLGKVDFVTIN
                     AVDTLIAGKMSESTPLTDVFEVGYYLKQMEQPDQNGNYLYYLMRRSDIIYDEDPLNGG
                     DSSYILSNVVDCKFEFGIAQKFTDKWDSREYRRFPNSVKTTLIVKNPNGNDEKFVFIS
                     YINLSR"
     gene            25134..26285
                     /locus_tag="PLV81_06155"
     CDS             25134..26285
                     /locus_tag="PLV81_06155"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspK"
                     /protein_id="HOM09720.1"
                     /translation="MFILIKKSIKKLFQKQTNHGYVLIIVLIIIAAIISISSEFLILA
                     QTNTRYLQSFTNRQKAYWIARSGINLAIELLEADKRGSIANFLSGDIETDPNVDSYKD
                     IWALPLPEIPLENGSIQLLIEDENSKINVSVLANEFVDQTPYYVITQRFFINMGLPID
                     LADCILDWVDIDDARSPYGAESSDYYQNLKKPYKAKNAALDTINELLLIKGISPLIFY
                     GLGGGNYGLEGNLVENNKGLQNVIESLTSGTKIEISKDTSLIKIGKEKNRALYNYFRA
                     NGERSDYLNDINKININTASFRVLSALTDAMTDDKVTEIIRRRLQKPFKNVDEISDII
                     TDETIRKNLLTVRSYIFKIKSIGKVGSTSLSIVAYYHRERKQIINWSEE"
     gene            26301..27932
                     /gene="pilM"
                     /locus_tag="PLV81_06160"
     CDS             26301..27932
                     /gene="pilM"
                     /locus_tag="PLV81_06160"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOM09721.1"
                     /translation="MFENIAVLDIGSFSIKCIKAKTGFKNFQVESLIVEDLDFSIDNK
                     LERITSGIDKILSQNDLTDYTVISTLSSNKLFFYTLNFPFKNIRQIGEVIGYEIADSI
                     PLDMDSVIYDFQPIQTKSPEGMDVIAVVAQKTLIHQITEIFAKYNLNLAFIGVEFNSL
                     FQSYNYFNTVQNESIIQLNIGYEKSIINLIINNSLCATRCILFGVKNIEDHFNKILTE
                     HYGIHQFIVNHSFDLSSFENNIHYGLHKKLNISQQKFKTIFNDIQNEIDNLINEILLF
                     INAETQKYENFSLQRIIISGGGALLTGFSTVLSQQLQIPVVLQTLMDQTGNTEVQSQF
                     YITFGTLIDYINRSKQKTIDLLKDDFQLSLSQQSKKNFYIALFYGSLSILFLVLFIIL
                     SLIYQLSSSSYYNRILEDRYKRYFLTNIVPDDPIKEATNRYATLKREIDSIESFLKID
                     DKMIEILNLLVSNFPYDANFDLNNMVINESIIRLDGSINSTAKIDEFKNRLVQTQKFE
                     SVVFNTNVMQNNVKFSMTIKFKSNKPKRQTTANED"
     gene            27935..28453
                     /locus_tag="PLV81_06165"
     CDS             27935..28453
                     /locus_tag="PLV81_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09722.1"
                     /translation="MIKLSNREKLLLKILIGILILIIGYNFIFTPLINFFSSSDTLIV
                     SDKQKIEQLDRIYSNYKELETKRNQYMSLLKSDSENISSMIEQWAQSSNVAKNIAYSR
                     RNQTNVQNKYIRITTDMKLDAVSIQSLLRFIYEIENSNKLVNISYLRINKSYKSPNLY
                     DVNLKIESFTLQ"
     gene            28471..29310
                     /gene="gspN"
                     /locus_tag="PLV81_06170"
     CDS             28471..29310
                     /gene="gspN"
                     /locus_tag="PLV81_06170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HOM09723.1"
                     /translation="MNLSNYKIILKTYFDRLYNFLKEIVTVQYFWRYLVFSIVMVILF
                     VFLTFPYEIIIQNKIKEVEQNIANYISIGRLKIHTITDSIAEDITIELKNGTEITLQN
                     VLFNFTINPFSIFITHHYDGTIKIHNLNLKGEKLQIITNADILYDLKYNKPLPYPAEG
                     NIISSMKDVSIKGISIQGFEIPDINISQIKSELIFEKGNMINLKYLLFSGSEIRGNAY
                     GTITLSNYFQNSSLNITIQIDSQSSILEDYKVLLESMMKAGTKNITIKIMGTIGNPNF
                     VTQ"
     gene            29332..31077
                     /gene="ispH"
                     /locus_tag="PLV81_06175"
     CDS             29332..31077
                     /gene="ispH"
                     /locus_tag="PLV81_06175"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOM09724.1"
                     /translation="MKIKIAENTGFCMGVRKAVLDIVDVINKNSETIYVIGPLIHNPQ
                     TVSILQSRGIITINSIDNIENKVIAIRTHGIPKESLRLLKQKASKIINLTCPRVARVQ
                     GIVKKYSREGYFAIIIGDDHHAEVESVKSYADAGVIVISNIEDIKKIPDALKYIVVAQ
                     TTFDECLFEEITQILKRKYNDILIFNTICDSTHKRQSDVINAVKEGIDTLVVVGGKNS
                     ANTQRLAQIGKNNNIKTFHIESEEELNDEYFKNSNYVLVTAGASTPGWIINNVLEKLY
                     SISNKYKNPLLYFIMHFFEIAIRLNLISSIASFFMSLLIEKLSGVWSSYKLPFLSAFF
                     IFIMYAINNYFLKDTLKITNPFKYKLHKNNHFIILTSTIAAILFSVMITLNIKNYLSL
                     LVYYSILLTGMVYSSSYFKFLINKYTSSLIKTIYSIKSIPADIGWIIVTLFIPVCYAF
                     QDSKLSEIHTGIVLSLIIFLSYLIVSRNLLLDFIGFNGDLIIGNVTVPIILGINKARY
                     LFYILSCMAMISLFYLSIFYNIKFIFFIINIMINYYLVLKILNLKYFISLKYEMLTDM
                     NLVIFILLCLFIIIG"
     gene            complement(31140..31328)
                     /locus_tag="PLV81_06180"
     CDS             complement(31140..31328)
                     /locus_tag="PLV81_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09725.1"
                     /translation="MKKEKVWVDVYSFYDTVYKNKPCIYGNIENYEAGTYKILIVDKG
                     NTIAEKEFVIYNENENID"
     gene            complement(31810..32538)
                     /locus_tag="PLV81_06185"
     CDS             complement(31810..32538)
                     /locus_tag="PLV81_06185"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOM09726.1"
                     /translation="MSHVQLCVNVDHIATLRQARGGKEPDPVQGAIICEEHGAAGITV
                     HLREDRRHIQDHDVFALRKVVKGKFNLEMALSDEIIEIALKVKPDQITLVPEKRQEIT
                     TEGGLDVKTNFKKICEIVSLFHKENILVSLFIEPEEESVKLSKESCADFIELHTGTYC
                     NAKGKDTIDKEKSRLFYAATIASNIGIRVNAGHGLNYINTAPILSMPGLEELNIGHSI
                     ISRSIFTGLANAVSEMVRIINENN"
     gene            complement(32560..>33027)
                     /locus_tag="PLV81_06190"
     CDS             complement(32560..>33027)
                     /locus_tag="PLV81_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09727.1"
                     /translation="LIQISGADIIVDAIKSLKTYPLNIDKEIQTLHVPVHVNIKDYSK
                     IFISPDTVMLTIPITSVSNLVSLTMPIEIRNQRNDFKYILKHKKVVVYIKKSLMNIEL
                     NENVLVAYVNGVIDANDQEVIKLPIFVEKKVKAPFEIFTYEPTETEVYISHEE"
     assembly_gap    33028..33127
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<33128..33925)
                     /gene="cdaA"
                     /locus_tag="PLV81_06195"
     CDS             complement(<33128..33925)
                     /gene="cdaA"
                     /locus_tag="PLV81_06195"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOM09728.1"
                     /translation="MNPFFTTAWDYIKISWEYVRIIIDILIVAYIFYWIYTFLSRSRA
                     IQILKGLIIIFIAAVLSKVLRLETLNWLITNLTSYIVIMIIILFQPELRRIITQFGQR
                     TWLPTSMNTDAFPLDELVNAIIAMSEEKVGSLIVIERGTDLRGYIESGVVVNAGITEE
                     LIRTIFFPNTPLHDGAIIIKEAKIAAAACYLPLSDSRQLKKQHGARHRAALGMAEETD
                     ALVIVTSEETGNISIMVNDKFQSKIKYTDLKNMILFYMNPKSKEEIYN"
     gene            complement(33922..34749)
                     /gene="folP"
                     /locus_tag="PLV81_06200"
     CDS             complement(33922..34749)
                     /gene="folP"
                     /locus_tag="PLV81_06200"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOM09729.1"
                     /translation="MNRIDINIKHGPLVMGILNVTPDSFYDGGKYNSIDAALYHVREM
                     INDGVDIIDVGGESTRPGAEEVTLEEEIDRVCPVIEKIIHEFNIPVSIDTRKSRVADE
                     ALRLGAIMVNDISGLQGDPDMPVTIARYNAYCVIMHIKGTPQTMQLNTEYNDLLSDIA
                     RYLCQSIEIAKKAHIADDKIIVDPGIGFGKSLEQNYVILNNIQYFKKLGYPVLIGLSR
                     KSLIGKLYDTHEDRLPATIALNAIAMYNGADIIRVHDVKEHKLIIKPLKMLYQVANS"
     gene            complement(34746..>34985)
                     /locus_tag="PLV81_06205"
     CDS             complement(34746..>34985)
                     /locus_tag="PLV81_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09730.1"
                     /translation="NNFTIAFSGIKKIEQDDYEYNIGFELLVYKSMSVYLGLNDSLFT
                     TGCTIGLGDCTIGYAFSYDSVDLGYNNFISVMLVL"
BASE COUNT        12011 a         6036 c         5303 g        11535 t
ORIGIN      
        1 aatataacat tttgttatat tttctgttat atttgttata tttgtcaata gttattttta
       61 aaaaattata ctgatttaat aactaaaata tttatttcag aataaatatg tattgaattt
      121 ttattgattt atttataatc cagacacttt tatgagttga atcaaattat aatcattatt
      181 tataaaataa tatggctttt aaagagattg gtaaaaaaat acagatagcc cgcgaagaga
      241 agggcattac tcagattgaa ctggctaaaa tccttggtat tacgcaggct gcattgtcaa
      301 attatgaatt aggcaagcga agagtatatt ttcatcagat tgaattaata gccgaacatc
      361 ttgaaaaacc aatttcatac tttcttgatg atgaatcaat gttagaagat gatgaaaata
      421 taattaatga tgaaatgtta gatttactta ccagtaaggt aaagcaatta aacaggcagg
      481 aattttcaga attagaccaa tttattgatt ttcttctctg gagaaaacac aatgagtaat
      541 attttgttac cacaggacct caatcaatct ttacatgaat gtatacaatt gcttgattca
      601 caatcttctg taataaataa tttgtcaact actgaacgtg tactggctta ttattcaata
      661 tattgcaaaa ataaattaca tgtattatta ttacctgaag aatatggtgg tctgtctgga
      721 tcatttagcc atgctggtat tattgttgaa acacttgcat ataattatta taacactttg
      781 cctactttat taacaactat acactgctat gaactcataa agtcgtttgc agataataac
      841 aaaaagaaat ggctacttaa tgctgttaac aacacaccaa agccactagc cttttgccta
      901 accgaagaac atgcaggttc cgatataaca tccattcaaa ctactgcaat aaaaaatgat
      961 aataaaatta taattaatgg gaaaaaggtc attgttatca atagcggtat agcatcattt
     1021 tatttagtta tagcacaaag cagcaaaaag ggaagggcag ggcttactgc tttccttatt
     1081 ccagaaactg ctgataacat ttcatttgaa aaactcgaag tgtcttcatt tttgcatagt
     1141 gtgataggta ctgttacttt taataatgtt ataattgata cacaatacca gattggcgat
     1201 gaaggaagtg gattcttttt actaactgaa atgcttgata aaggaaggcc tcttgtggca
     1261 gcatgctgtg ttggtgcagc tcaacatgca ctggatatca ttattgatca cacaaaaaca
     1321 agaaaacaat tttccaggta tttatatgat ttccagggaa taagttttcc tatagctgaa
     1381 tttgaaacac ggttgcatgc agcacgttta ttatataacg atgcatgtga gaaaatagat
     1441 aataatgaac cattcaccaa agaagcttct atggcaaaat actatgccag cagattgttt
     1501 cttgatatta ctaactttgg aatggatacg ttagggtaca ggtcaattat tgatgcaagt
     1561 gaaatgaagc tattaaacga ttttgcaaaa acaatgatgt ttattgatgg aactgaaaat
     1621 gtacaaaaga tgatcattgc atctttacta taaaacaaca gagatttttt tactctaagt
     1681 atacaacatt atcatactgg ccatataaaa acaaatgttt ctttatttcc ttaattctat
     1741 tatttaatat tttaatttcc tcactattag cattattatt ttcttgcttt ttataaacat
     1801 attgttttat ggcaatcggt tgtattcgtc ttccatattt atattttgat tctgtgtcag
     1861 gtacattata aatcatgata ggaagaatat tgaatttatg atatattcca ctttgatcct
     1921 ttcccaatat taaactaaca attaaacctt cacgggtact gcattcaaca ccgctttttg
     1981 gatcattatg agttgatgac tggtttgcta tgaaatttcc caggctataa aatactgtac
     2041 atattcgtcc atcagaagct ttaatttgtt caacaggctg tattacatgt ggatgatgcc
     2101 caataacaca atccacacca gcttcgcaat atgttttgat tatattcata tctttttttg
     2161 atggctgagt actatattca ttgccgatat gtgcaatcat tataagaaaa tcgcatttat
     2221 tttttatttc cgcaatatct gttaatactt ttttagtatc ataaaagtcg ttaatatgta
     2281 tgttattttt atattttttt gatagaccat aattcaaaac cccagtgtag gcaataatac
     2341 caactttaac ggggttattg ccaacaataa ttccatttag tgttgaatta tttttactga
     2401 cacccatata ctgaatatgc tttgattcca gtatctggat agtgctgatt aatccatcat
     2461 acccctgatc cagtatatga ttatttgcaa tactgactat attgatatta atgtttttga
     2521 atgcatctaa aacatacggt ggacaattga atataaatgc cttgctggta tatggtggag
     2581 caactggaaa ttccatattt gccaccacat aatcagatga ttcaaactca tgttttatat
     2641 gttcaaaaag tatgtcaaaa ccaccattgt ttattgatct ggcagttttt ggatcattta
     2701 tattatttct aaaagcaaat gattttacag ggccatgcat aataacatca ccaataaatg
     2761 tgatagttac tgtatcattt agctttgaat gtattttaga acaatagcta catgtaatta
     2821 ctaaaactaa tattaaatat attttcttca ctgctaaatc ctataatgaa tattaatgat
     2881 atgtatcaca caatcgaata agaagtaata ttgtaactat cagccatata ccgtaaaaga
     2941 aataattttg tctataaaca tttatccttt tatacttcat atattacttc cgataagata
     3001 tattatgtca cattagagaa ataaagatag tatatttatt acattactta ttgcatctta
     3061 taacatagta tataaacaat aactatcgcc catttgcttt ttacagtatt tacttatatt
     3121 tggattcagg gtcgttactt atttaaaatt tttattttcc atatctttga tttcatcaat
     3181 gcagtttgct aaaaattttt gccattcttc ttcaaaaccg ctcccaaatg gtgttgaaag
     3241 ccagtcattt attatctcaa atgcatgatt tgcgggtgta agccattcag ccagtaccag
     3301 tacatttgca ttgttaatag ctgatgcttt ttttgcttca aatgaattgg tacaatgtac
     3361 cgcatataca cctttgaatt tattggcaac aattgcacta cccgctcccg taccacatat
     3421 tacaatgcct ttttcatatt ttccctgtga aaccaaggat gccacatttg cagctgctat
     3481 atgatagggc acaaaatttg cttcatcttt cataccaaca tcatctattt gatgaccctg
     3541 ctttacaaga taatcaatta tagcacgttt aagctgcaat ccagcacgtg ttgatccaat
     3601 gacgaagttc attgtttaat tccccctatt tgcttttaat catatccttg catgcagtaa
     3661 taatatgttc tacattaaat ccatgtttgt tgagtaaata ttcttctgtt gcaacttcac
     3721 caaaggcatc aggaataccc agacgtttca ctattaccgg aagtttttca cttaatactt
     3781 cacagacagc cccacctaat ccaccaatga tattttgatt ttccacagtt acaacacaac
     3841 cagtcttctt tgctgattgt atcagcaaat cctcatcaaa aggttttatg gagctgtagt
     3901 gaagatgctc tgctttaatt ccttcttttt ttaattgctc tacggcattt atagccaaat
     3961 gcgttgtata tcccgttgtt acaattgtta catcgctgcc ttctgaaatt tttgttgctt
     4021 tatttggatc aaacgtaaaa ctttcattaa atactactgg catcttgggt cgcagtaatt
     4081 gcatataggt tggctgatga tgtgctgcca tatacttcat tacagcacgc agttcatata
     4141 catcacaggg gctgtataca tgcatattgg gcatagcacg cataattgct aaatccagaa
     4201 agtccatatg ggttccaccg tttggtccct gtgtaatacc gggcgcagtt cccactatct
     4261 ttacattggt attggcatac gccacactga tggtaatctg gtcatacacc cttcgtgatg
     4321 caaagcaggt aaaacttgca caaaatggta ttttgccttc acgtgctaaa cctgctgcca
     4381 atccgatcat atttgcttcg gcaacgccaa catttataaa attttcaggg aatgctgtac
     4441 atactgctgg attggttcct gaagctttac ttaaatcagc ttccaggcat aaaacatttt
     4501 tatcgtgttc catcaattca cataatgtta acccatacac tgcacgcatt tccatgtcat
     4561 gataactcat tgtttttctc cttaaacaaa tatattaatc attttgaatt aatcattata
     4621 ctgtaatggc cattgatcat tgattactaa taagcattat gcccttgcaa aaataccctg
     4681 tataatcaat atggcaacgt cattgtataa cccaaacctt tcatatactt atcataatct
     4741 gattgattat tgatcttaat attatggctg gccactttat tttcaacttc tgcattgcct
     4801 tttcctttta ttgtatgggc aatgatcatt gcaggctttc cttttactga aattgcttta
     4861 ttaatggtgg aatatatgtc atcccagtta tgaccatcca tttcaaatac ttcaaaatta
     4921 aatgcacgcc atttatcagc taagggctca agtgttacaa cttcatcagt ttttccatca
     4981 atctgcattt tattgtaatc acaaattaca atcaggttat ccagtttatt atgtcctgca
     5041 aacattgcag cttcccaaat ttgcccttca ttgctttcac catcaccaat aatacaaaag
     5101 acgttatatg actttttatt gagtttagca gcatacgcca taccacaggc acatgataaa
     5161 ccctgcccca atgaacctgc tgtcatgtct atacctggcg ttttattatg atctacatgg
     5221 cttggtaaat ttgtaccatt ctggtttaat gtttttagcc agctttttgg gaaaaagcct
     5281 aaatatgcaa gtactacata caagactggc ccggcatgtc cttttgataa aacaagtctg
     5341 tcacggtctt cccatgctgg attttcaggc ttaatattca tgatacgata atatagtgtg
     5401 gtaactatct ccactaaact caatgaacca cccaaatggc ctgaaccagc tctgcatatc
     5461 atgtcggtta ttacatatct gaactctttt gcacgttgtt ctaattgttc tgttgacatt
     5521 gtattcatga actcctccat taacagttac tatcgcataa aataattttt ttgaatatta
     5581 ctaattaaaa aaatatttac catgttatga tgttaaacaa agaaccaaaa attaaaaaac
     5641 ttattgtgac acatatagat gaaacaatgg tcatatatat gcccggcttc accatatctg
     5701 caattgtgaa atatcctgct gtataaggaa taacaattgt tggcgttgat gttaccagta
     5761 taaaagaaag cgatgatgtt aatcctgctg gaattgcaac cagcgcagga tcaatcccaa
     5821 tagtaccaga tagtgctatg agtaacggta ccattatgat accggttact gtattgcttg
     5881 aaaacataac ttttacaata gaaaccccaa tgaccactgc aaaaaccata ataacaggat
     5941 gtaaaagatt aagatttttg aatagtaccc atgcaagcca tttagctgct cccgttttgt
     6001 atattgttgt tcccagcgct aaacctgaaa caattaaaat aattgcgccc cagttaatag
     6061 catgttcaac cttatgccag cttaactgcg caaaaggcgg caaaaacata caacatgcaa
     6121 ttgttatagc aacaaaggac atagttatat actgtaacga tgggaataga ttatgcagta
     6181 caggttccac tatccataac aatattgtaa gaataaataa tatcccaatt attatatcat
     6241 gtaatgggat ttgtattttt tcattatgtt cagtatctac atgtaaactt accgattcta
     6301 tgttaaacgt ttttaccagt atataccatg caaaaggtag cattaacagt gctgcaggaa
     6361 aaccaatacc catccattgt gcaaacgaaa gatcaatatg cgccagatcc tttaaaaatc
     6421 ccacggtaag cggattagca cccgaacctg caggggttga tatgccacct attactggtc
     6481 cccaaccaca tgcaatcatc attgctttac cataattatc tttcagtggc tctatatgtg
     6541 ctcgttttaa gattgaaact gcaaaaggca acaccatagc tgctgccgcc atttctgtca
     6601 caaatcccgc taaaatcatg ccaatagcaa gtataataaa aatcaatgtt ttaggttgtg
     6661 aacaatatcg tttataaagt ttttgtgtaa ttattgaaac aaaggaagtt tcattaacga
     6721 tagctccaat gatcataact ccaataaaaa aaacaataac ctgattgcca aaaccatctt
     6781 ttaccagatc agcaagattt gcacatcccg ttataaccag taacgaaagc gaaaataagc
     6841 ctgtaattgc aaatggcatt atttcagtta cccaccctat cactgcaaac gctaatactg
     6901 ccagagaata tttaccctga gttgttaatt gtatggattt atcacctgtg ttttgccaat
     6961 actcaattgg caacaaccct attgccaaag ctattccaat agctaaaaag aggtatagtg
     7021 tttttttgtg agatagtttc atagtataga atttaccgta ataattagat acaataaatc
     7081 gatatcgata tattatgtca attgtaattt ataataatgt tgcagtataa tagtatgtat
     7141 tatcaaaatt atattttatt acatcgccct cttcacacat ggcttccagg tgagtatatg
     7201 tttccgataa tgataaatac agatcaagca ccagatttat acctttaaga tttttaaata
     7261 cctggcgtgc cagtccataa acggtataat cgttattcaa taataaatta tacatgcgtt
     7321 tctttcgaaa ttgatatact ttctgatatt ttactatcaa acgatttaat ttttcctgaa
     7381 aaggttcacc atgtgcagga aaaatgtatt ggggacttaa tttttcaatc tttttcagtg
     7441 aggtatgaaa gatagtatga ctttgggcaa ctggaaatga tgtatttggt tcaaagcctg
     7501 ccagaataat tggctttaca ccaggtacaa tgtgatcacc agaaaataaa aacccttctt
     7561 tttcaatata caaacacata gcacctttgg aatgtcccgg tgttaagatc aatgtagcat
     7621 tgtaattccc aacctgtaat gaacattgat catcggtaat ttttcgtgta acaataacgg
     7681 gatgacccat tgatttaaat gttaaatcaa gaaatgccag tggcactatt aatggcttag
     7741 gtacacccat tgattcaatg aatttcaaca taatagaagg tttattgtac aagccttctt
     7801 ctattcgttt agcatctgca atgtgagtta ttacttcaac atgtttattt cttttagcta
     7861 ttgcatatgc tgcaccatag tgatcaacgt gtccatgtga gggaataact tgatgaatat
     7921 cagaaaattg tagtcctaaa tcatgcaatg ctcttcgcaa caaccgccat gtccagaata
     7981 ttccggtatc aaatagtgta ttaacttctc ccttaaaaag atatacattg atataaggcg
     8041 ggtaacccgg gagaggtaat gaaattctga aaatggtatc attaattggt gaaattttat
     8101 atgaaagctt caatggcatc ctgttgtcat taaagaattt ttcattaata tatctaattt
     8161 tactaatgat tccttaaaat ttttacaatt gattgtgaaa taaataactt taagaattat
     8221 taatttataa atttataaaa attattaaat tatatagctg tcaattgttt tatcttagaa
     8281 aattaatgca ttgtaataac atgaaatgaa taattgttga cttatagcac atatatagta
     8341 tgggcatgtt tttctgattg tcatagtttc agatcacaat ggggacaggc gaaaattgtt
     8401 acaatagcta ctgtatctta tttgatatct tttattgcct caaaaatagt tttaaactgt
     8461 gattttttcc tgtaaaaaac aggtggttgt cctaatgggg cttcaacctt aacccatcca
     8521 ccactatatt ccttccctga ccatacatgt acccattcat cctttggaag atatacgttc
     8581 cacttttttc ttcgtggcct gtacacaggc gctaccagaa catcacgacc cagtagaaat
     8641 tgatactgca acgaatgcag cttttcatca tcaggataat ggatgtaggg atggcgcatc
     8701 agcggtaatc catcagtatg atactcatct ttacaatgct caaaatatgg ttttaagatg
     8761 gtatggactt ttgtcatttt tgctaaatgc tgcagcgttt ccatatcact gttaaattgc
     8821 aagtttacat caggcctatt tccttcatgt gtcctcatta ctgctgtgaa cgcacccagc
     8881 tctgcccagc gcataaaaag ctctttcttt cgcttaatcc agcccaatgt ggtatatcca
     8941 ccaatatccg cgtgaaaata tcccacaccg caaaatccca gtgaaatacc tgcaggtatc
     9001 accgaagcca gtccatcatg catgctccag ttaacgagtt gatccccagc ccacatcaat
     9061 gtgctgtatt tagatgtacc tgtatatcct gcccgtgtaa aaaatactac ctcacccagc
     9121 ttacctgctt cttctaatgc ttcataattg acacgtgccc aatctgcggg atatttattg
     9181 tggtacaatt cagcagattc ccctgaataa agctttgcat cggtttccag atattcacca
     9241 aaatcggcca tccagccacc aagaccaatt ccaatcatat gctctttaat aacattttta
     9301 atccattccc gcgtttgggg attggtcaaa tctaacgtgc cagcaattat tccagtagta
     9361 tcaaacgttt taactgtgcc atcctgattg tgtaccaaat accctttgga aatagcctct
     9421 ttatacaggt caccctcaga atttaaaaag cagttaatat atcctaaaaa ttttatattt
     9481 ttgtgcgata gttcctgtat atatgatggc aggcctggat acaattcttg attatatttc
     9541 cagttccaga aaagcctctt gccaaaccag gttattcgtc ggccaaccca gtcctgtacc
     9601 cacagcgctg aaaccttaac tcccgcatca agtgcaacct gtaatttttt cttcacaaca
     9661 tcggtgccac cctgcattcc tatccacatg ccatcatgca cccactgagg tagccgtggc
     9721 tgtcgcccca ataaatcccc tactgctttg atacattcag ctggtttttc atacacacca
     9781 actaccactg attcaggaat ctgccagaaa tgaagtatat gctctttctt tttaaattca
     9841 aattctgcat atgacgatgc attgcagtgg ataaagtaat tgctgctcga tacaaacgtt
     9901 ggttgcggat aatacgttgt gtaccatgca cctccagcac catatagtac attggctaac
     9961 aatgttatca gatcatgtcc ccgccctact ccctgctcct gtacccatag gggaaggttc
    10021 tttttcttta aatccaactt tgaaaactgt tcaccacaac catagatata ttcatgaata
    10081 tcggcaacac aatgcaacca gaatctatta aaaggttttt ttgctttaat gttaatagtg
    10141 caatatgtat ctgcagttat tgtcaactct atagcatctt gtaattgtat tgtaatattg
    10201 tttttatctt ctttaacagt aaaatgtttt aaatattgtt ttgacgtaat ggaatctttt
    10261 agtttaaaat tgccttcttt catgctatag actgcgttac caaaacccac cgcaatgcat
    10321 ggctttttta cagagtgcat caagatacat cgattcttgt aatatagtga aaacgatgaa
    10381 tcattgtgtt caatttttag catgtcacta ttccccagtg ttgaatgcga gctgctatct
    10441 caaatggttt tatgaaatcc ccgtacacca ttgatgcgtg atgagcaata ccattataaa
    10501 taatggtatc aagaacattt cgcacaggca catcaaaggt aacttttaaa tatgtccctt
    10561 ttagcaattt atccattgta acgccatgag ctttcaataa taataagcga tagttactgc
    10621 catcaaaatc aaatctgcat attgacacat caccaggttt caatacaaaa cctgccgtaa
    10681 cacctttgcc agcagcaaaa tatgtatcaa gcgatgctgt gcttgcagta tctttgagtg
    10741 tgaatggcgc aacaccgcaa tgccatagca gtgcaaaatt ttcttcaaga tttacctgcg
    10801 aaaaatcaaa taagaatggt gtttgcgcac ccagcgcttt atgagctaac atggatagtg
    10861 caccatcaac gtcaccttca caggcaatga caaaattctg tgcctgtacc agcgacatgg
    10921 ctgcacaagg cgcaatgcca aattcagcgg caaattcagg ccagcaccga attgccagag
    10981 cagtaaggtt attatcttcc ataaaactgt gtagcctgga acttaattct gcaacctttt
    11041 gtttctgtgt gctattgata tcacctgtat caaaattact ttctatatcg tcagcatatt
    11101 ggttttggta ctgcttatta acaggataat tgaatacatg cggtaactca taatgttcaa
    11161 tcattgtgcc acactgtgca tacaatgcag gatgccaggt atccaggtta aaaaatccat
    11221 gggcgtgata gccaaccagt ccaattttgg catttttaag catggaaata atccgtatag
    11281 caccaatcca gtcagtatct atggtgttac caaaatgagc atagtagttg cgtatccctg
    11341 ctttatacag gttgcttgca tctaaattta cgccacaaat tgaattgagc cgtattttac
    11401 cgccatcata tggcaattca ggcaatcccc acagcatcac aggaacactg acatgtttta
    11461 tcaattccag agctaagtgc cctaaatgaa atgttccact gatacacaca agagcatcta
    11521 tattgttctt ttttaagaag gcaatagcct tttgtgcatc ttctacttca aatacaagtt
    11581 cattgtagca atgccactgt acagctttaa tcgtttttaa ctgtgattgt atggaaccat
    11641 atatttcttt agctgcactg taatcaaagg ttttacgagc aaaacataca acaccaacag
    11701 ttacttcttt cattattatc acctattgct ttttaaaaag attttcatat ttataaccgg
    11761 ttaatacttc acaaactttt ttaacaaatg gttcaactgc atctttgctc ccaccggatt
    11821 ttaatctgtt aatttcctga ataagaattg gatgtgttac cggtgtaatt ttaaatttta
    11881 atgaaatgag aataccacag agcataagcg ttgatggaac cagtgcaaac atcaagcgta
    11941 gatactctaa tgtttcaggg gattgaacgg tatttgcttt atatccaata gtttgcaaca
    12001 ctaaaccaaa tatagccagc acaactgcct gtaccaattt tcgtataaat gtcatagcac
    12061 ctgcatatac acctgcctgc tgtttcccgg ttatgagttc ccctacatcg gtcactgaag
    12121 gcaaaattgc ccagggtatc atagttcctg ccgacatacc agcccccatc agtactgaaa
    12181 gggcaacgat gtattgtagc ggagtggcac tatcaaacaa aagagtactt acaagagcta
    12241 tcgcccatat acttacccct acacgatagg aaaaaccctt acctttttta tttgcaataa
    12301 atacatatac gggcaaaaac aaaacctgag tcacaagcat tgcaccaaga cacattgtaa
    12361 atatatgtgg tttttgaata taataggtaa gatagtaaat gaacattgcc atgattaaat
    12421 caagtgctga atatgcgcag atatacaaac caatatgaac cctgaacgat ttgtttgtcc
    12481 atataaccaa gagatttttg agaaaatcag taagagattt attttccacc tttacatcag
    12541 gcagttccca tgttccaaaa aatacgataa tccatggcaa cgcaaaaaaa actgcaaaaa
    12601 caatactcat tacaaggaaa ccctgcccct tatccgtaaa tgcatcaaca accattttag
    12661 gtacggtacc tgatatgagt gatgatattt gagagaaaat taaccgtgca cctgagaggc
    12721 gcgtcctatc tttatattct cgtgtcatct cagcattgag tgctgaatac ggaatcatca
    12781 ccatggtaaa ggctgtacta aaaaatacat acgcacaggc gtaataaata aattttatta
    12841 tctggctttc agatgaaaaa ggcagccaca tggcaataaa actaagtatg attgggaaaa
    12901 tccccagtaa aaagaatatt ctgcgtctac cgtattttga tttagtagta tcagaaatat
    12961 atcccatgat tgggtcactg attgcatccc atgccttacc agccagcact acaagtccag
    13021 ccagtgcagg attgagtttt gctatatcag ttaaaaaata tataaataaa agatttataa
    13081 caacaaaaga accaccacca aaaatatcac caacgccata cgataataaa tttttaaaag
    13141 ttatttttct ttcttgattc ataagcgcac ctgttttaaa ttataaaaaa ttgttgcata
    13201 agtaaaaaca atgagcaata atgttataat atcaactatc attttgatag tttaataata
    13261 aaatcgttat cgataatatg tcaagaaaat tatcatactc aggaagaatg ttatggcaca
    13321 tggtgaaaaa atcaacaaac gaaaagaaaa taaacgtaag catatagtcg atatcttccg
    13381 gcaacatcct gtattatcaa aagccgaagc aaaacgatat tccgggtatt ctatggatac
    13441 catcatgacc tcttttaaaa ttttaaaaaa tacaggtata atagtaccta ctgaaggtaa
    13501 acaaaaagaa aaaggccgta aagcattatt ctatactcta aaccatgaaa agcaggtata
    13561 tttaggtatc acctttaatc aaaaagcaat atacacatct atcgttagtt tcagcaacaa
    13621 agttttagcc aaaacaaaag ttgggatttc cagcagtatt tcaaaagaag aatttacccg
    13681 ggttatgcta caacaaattg acgaagtgtt atcaaaaaat cctgtgttta caaacaatct
    13741 tgttgctgta ggttgcacag tacctggcga tattgatacc agaaatggaa tcttacgatc
    13801 atatacactc atgcctcaac tgaaggattt taattttaat gaactgttat ccactaaatt
    13861 tcccaataca cgaatatttt ttgatcataa tatcagcagc atgatttcat acatcttatc
    13921 aacttctgat attaatcagc gttacgacag gataatggtt gtttcagcac gtgcagcaag
    13981 tgctgttgga ctaatttata aaggggcgat agtaaacagt agaggggaaa ttggccatat
    14041 caaagtctgt gatgataacc accaatgtat ctgtggaaga agtggatgcc tggattgcta
    14101 tttttcctat gaagcatttg tcaaattatt acagcagcaa aacgccatag atgaaaacga
    14161 atgctacgat aatgtaatag caaaacttaa caaccattat actaatagaa cagaaggtat
    14221 atttgaaaag cttgatacag cactgaagtt tttttctgaa gcattacttg atgtaactaa
    14281 tgttttctca cctgattata ttcttcttac tggagatttg tttgaaatat acaatgatcc
    14341 tgttgcccag atagatattc atatcaaaga tctatttcat gataccggat atatcaccat
    14401 gtacaaacgg gcattaatgg aattccagag tataaaaact gaatcaatag ctcatggggt
    14461 atgcttgagt ttaataaagc aggattgggg atattcagca taattacttc ttttgtaata
    14521 ctataactgt tacatcatca cctaatccta catcacccct gaattcatta aacgttttaa
    14581 tgatataatc acaaatttcc tgtgcagttt gtccctgagc atgcaaaaaa gcttcttgaa
    14641 gcctctcaaa tccaaattct tcagcatctt tatttttttg ttcagtaagc ccatcactgt
    14701 ataccaacaa ctgatctcca ggccacatgc taaattgaag gggtgtatag gtagtttgga
    14761 atacactttt ccctaaaaac atccccttaa tatctctttc tttatgagta catacaataa
    14821 cattattgct ttctttacgt tttaaaagca tctccgtatg ggcagcattt acatattcaa
    14881 ccttaccatt tttaaaacgt aaaagtatgc ccgttaaata attatcaaca tgttccagtt
    14941 cctgtattaa ttcctgattg ataaattcta ttactttgcc caaatgcaga ttaattagtt
    15001 tcatgaaatg acgaaaaata attgattttg acattattgt taacaaaccg gaggcaatac
    15061 cgtgcccgga aacatcaaat aatccaattc caactaattt atcattttca atataaaaat
    15121 cataaaaatc acctgatact cctgacattg cctgagcata tagtccaatg tcataattat
    15181 caaccacagg tgctttttgt ggtataatac tacgctgaac atttactgcc attatcatat
    15241 ctctctgtgc aatttcctgc tgttcacgta tctggtttaa tgcaagtttt aattcctgat
    15301 ttgcttcata taattgtcgc gtcctgtcct ctactttttt ctccaatgta acatttaatt
    15361 cttccacttc attatgatac tgaactgttt gaattgctaa tatcgctgct atagaaatta
    15421 aaaatgatgg gaagccaaaa cccataagat atatagcatc atcaatcttt aatatattat
    15481 ggacaataag atcccagaaa gcacaaaaat aaaatgggat actacctatt atcaaaattt
    15541 tagcttctct tttcttttgt tttaaagcag caaccgtaat agaaattata tatatagctg
    15601 gtatgatgat aaataggatt actttatatc taatatttaa aaaggttgga taatcttttg
    15661 ccagtaataa tattatagat ggtacaacag ttgcaaaaat taataaagct ttaataataa
    15721 cgttatcaga aatatttaga taccttgtta aaaaccttga taataaatat gctatcacaa
    15781 aaattaaaac aaagattatt ttttgaaaca aaagatatga tataagatta taatggaatc
    15841 caggtagacg ggtaacaaaa aaatttatta aatataaaga aaatgcaata catgttaatc
    15901 caaaatataa attttctatg tcttttttac gcaatgaata catcagtatg taataacaac
    15961 caactaacaa aaaaagaaaa gaaataattg cgtttattct tgaatctatg aagtctaaat
    16021 aatcgtattc cttttctaat tctttataat taccaataat attttttcca gcgatagatc
    16081 cttcatggtt aacataaacc ttaatcagca atacattatt ttcatttgtt ttcaaaatac
    16141 tttttggcaa catatataat ctatatttat tccattcact aaaaaaattt ggtggaaatt
    16201 gtcccgtgat accaacaagc tcaccattac aatacgtttc atcagctatc attattcttc
    16261 ctaacaaaac tcctattaat ggtgcattta ctatttgatc tgaagcagta aaatcactgc
    16321 gtaaccatac aaatccttcg taatcgggaa ctaatgtatg caaattatta gtagcgttta
    16381 acggcttaaa tggcattttt atagcatcat gatagccctg ttctgacttg ccggtagcat
    16441 atttccattt cgacaggtaa agaacctcgg caacaactaa tgattgggat attatagata
    16501 ttataattaa tatcaaaata aggataataa ttgaattgtt ttttttaatc atcaaggaat
    16561 tcctcctgaa aatgcattaa acctttacta tctcttttat taaatccttt tacattagta
    16621 caaaaaaaat taatacatac cttattcatt tccatatatg ttataacttt actttcagaa
    16681 aatctaatta cagcaccacc aatattcgta tatatataaa aaaaatcaat attattatcg
    16741 ttatcgacat atatgctaat aaaaatttta cttaactggt atttcaattt atccgacata
    16801 ataaatatat gagacataaa ttttattatg cgaggtactt atgccagcaa aaaagaagtc
    16861 caattcattt ataaagcagc taaaaaaata tatctcaatc aaaggattgg aaatagtaat
    16921 acatcttgta aatggagaag tcattgaatt gcagaataat gtacgcctgg aaaaaaataa
    16981 tattgtcgtc agaaataaaa acagggaatt tcacatccca atttcagata ttaaatcaat
    17041 tgacctgtat gcagcataaa tatttttaat attaatacta ataattattg acatcattca
    17101 atatttatat atcaattttt gtaactttac aagcaattca ctatacttaa aatgtatagt
    17161 gaattgtgct ttatatatgg ttttaattta ctattttgtt ttgtttattt tttagattat
    17221 ctaatgtatc aaacagagca tgcaccgtga tttttatcgt atcattaaat ttgtgtaaag
    17281 aatactgaat atgaccagga tacatataca agttattaat ataatcagtg tgatcctgtt
    17341 ttcttttatc actgctcagg ttattaatca gagtataaaa tatttcataa aacctgctga
    17401 ttttaaaaat tatatccatg caaggtataa accagcaaca acatcgttaa agagttttaa
    17461 tgaatatcaa tcaatgcttg attctaattt cttcaaagta gctacagtag atagttctgg
    17521 tgcagcttca ataagcagtg aaataaatga attagtggta ttgggaacta tctctggccc
    17581 cccaggtatt gcaagagctc tcataaaaaa acaatctgaa agctctgcac aaatatataa
    17641 gattggtatg gatgtatatg gctttaaact tgttaaaatt gagaatgcta aagtcttttt
    17701 aaaaaaagat ggaaaagttg aaattattga tatgtttgca aaagataaat catcttcttc
    17761 aagttccgga ccacaattat cacaacaaag ttctccaaat agaatagtaa aatctatcag
    17821 caagtcagaa attcagcaaa aagtattaaa caatcttgac aatgcactta atggtgttag
    17881 agctggacca tatcgtgtgg gtggaaaaat tgaagggttt aaactttttt ctgttgctcc
    17941 ttttaatatt ttgtattcaa tgggagcccg tagcggtgac atagtaaaac gaattaatgg
    18001 gcaaccaatt gatagtactg aaaaattgat gaagatgtgg gaaacattaa aaacagatac
    18061 acgttttgtt attgaactgg aacgctcagg ccagatgatt acatatgatc tgaatgtttc
    18121 ggattagaat acatatacta ttaaatacat ggctttactg gtggttcaat gaaaaaaaat
    18181 aaattaaatt ttaattacta tctgtttatt atactttcaa gcttattcat tatatcattt
    18241 ctttatgctc aacaggaaaa tactaatcct gaaaaacctg ctgagaccca gactccacag
    18301 gctgaaatta tcacaccaaa agatgaacaa aaagaaagtc aggcaaaacc agaaatcaaa
    18361 attaaaaaaa acaaaggaaa aaagaaatca aaggaacagt attttaccct taattttaag
    18421 gatgtggagt tgtcagaatt tttaggtgtc atgagtcagc tgattgggaa aaatattgtt
    18481 atcgatgaac gtgtcagagg taaaattaca atttcatctg ttaagaagat acctgtcaaa
    18541 gatgcatacg aagtaatgaa agcaatcctt gaattaaaag gatttgcagt agtggaatcc
    18601 gagaacctta ttaaaatact acccataaaa gacgcaatta aaaagaatgt tgaagtaatt
    18661 attgatacta caaaaaagca gattgttcct tcagatgacc agaccattac ttatttacag
    18721 gaaattaaaa atgccgatgc agaacagatt gcaaatgttt tgcgagcatt aaaatcgcct
    18781 aatactgaca ttattgttta ccgagactta aatataatta tttacagcgg tacagcatca
    18841 gaaatacatg gtttaatcaa aattgcctct tcacttgata aaaaaattca ggaacttgaa
    18901 gtcgataaaa ccatcaaggg agatattcac gttgtgcatc ttgaaaatgc tgacgctgaa
    18961 aagctggctg cagttttatc cagaattcct tttgcagagt atgctgcaat aagtaaaggc
    19021 gaccaatcac accaggttga tgttaccaaa caggcacagc aaattgcaca ggctaaagcg
    19081 gcaataactg ataaacagaa actatcgatt atagctaata aagaaaccaa ttcactcata
    19141 ataacagcta aacctgaaga attcaggatg ataaaaaata tcattgacca gctggatatc
    19201 gtacgtgaac aggttttgat tgaagcatta attattgaag tatctgcaga tagtggctgg
    19261 ggttttggca tagactggat gctgggtgac caatacagtt cacatcttta tggcggatca
    19321 tcaatacagg gaaccccacc atcatatact cttccatcag gtatgacagg caaaaaaatt
    19381 gccgtgccat tatcaacagg attccagatt ggttatcttt cagataaatc catgttgggt
    19441 tttgcattgt taaatgcaac tggtgcagaa aaacattaca atattctatc aacaccacaa
    19501 atcctcacaa ctgacaatca ggaggctgag atcaatgtag gggaagaaat tcctgtccca
    19561 accaacaata gaataagtga caccggtgtc cagttttata cctatgaata taaatctgtt
    19621 ggagtaaaac taaaacttac ccctcatatt acatcccaaa acagcataac attagactta
    19681 tatcaggaag ttaattctgt tcttggacaa acaacagtgc ttgaatcagg atcagtaatt
    19741 cctcctaaat tgggtaagcg ggatattaaa acaaaaatta atattgcaga tgggaaaact
    19801 atagtggtag gcggattaat taccaacaat aaaagtgttg aagaaacaaa agtccccatt
    19861 ttaggagata tccccttatt gggttggttt tttaaacgca aagttgttga atacaaaaaa
    19921 accaatcttc tggtatttat tacacctcat attgttacaa aacaatcaaa aattgatgcg
    19981 ttaaccgaac aaaaacgcga agaacaaaga agactacaat tagactagct atgataggta
    20041 ttaagaataa aaaaattggg cagattttaa ttgataaaag attgatcacc aatgaagatc
    20101 ttcaggatgc ccttctctcg caacaatcaa cccctttaaa aataggtcag atccttataa
    20161 aaaagggatt aattaacgaa tcagatttat tacaagcact agccacccaa ttttcaatgg
    20221 aatttgctga aacattaaat ttttctgatg atgaaaatat actcagtgca attcctgccc
    20281 agtttttaaa gagatataaa atagctccct actctgtaaa tgtaaaaaag aatattatta
    20341 aagttgccat aaacgatcct cttttgattc agccattaga taacattaag attatctttg
    20401 aaaattacaa aattgtacct gtcttatcaa ctgaaaaaga aattcaaaaa ataattaata
    20461 atcattatga acttttgaaa gaaaactcaa caacatcggt aatagaaaat cttgaagaat
    20521 ctgatttcga aattttgaca tcccccatta cagaaactga agacatcctg gatatggcca
    20581 atgaagcacc aattatccgt cttgttaata ctgtaatcaa acaggccata gaagaccgtg
    20641 caagtgatat acatatagaa ccatttgaaa aagatcttgt tatacggttt agaattgatg
    20701 gtattttata caacatgttt actccaccta agaaatatca ggcagccatt atatcacgaa
    20761 taaaaattat ggccaattta aatattgcag aaaaccgtct tcctcaggat gggagaatac
    20821 aattaaaagt tggtgggaag gatatagata ttcgtgtttc agtattccct acatattatg
    20881 gcgaacgcat tgttttgcga ttactcaata agagtgacat gaaatttgat ctggattctt
    20941 tagggttttc caaagaaaca ttaaaagtat tcaatgactt aataaaaaaa acacatggga
    21001 taatacttgt tacagggcca actggtagtg gtaaaacaac aacattatac agtgtcttaa
    21061 cacgccttaa tactgccgat gtaaacatct taacggtaga agacccaata gaatatcagc
    21121 ttcatggtgt tggccaaatg caggtcaagc caaaaataga ccttaccttt gcagcaggac
    21181 tcagatcaat tttacggcag gaccctgata taataatggt tggtgaaata cgcgacttag
    21241 aaacagccga aattgcaatc caggcagcat tgactggtca tagagttttt tcaactattc
    21301 acaccaatga cgctgccagt gggattaccc gtcttcttga tatgggagtt gaaccatttc
    21361 tcatcgcatc atcggtcaat gctttcttag ctcaacggtt ggtacgtact atttgtcctc
    21421 attgcaagca atcatacaaa cccgatcctg ttgtactcaa ggatctgggt attaagccct
    21481 ctcaaatcca gggaaaaaaa ttatatagag gcaaaggctg tgataaatgt atcaacaccg
    21541 gctatttagg cagaacagga atttatgagc tactccctgt taccaatgac atacgaaagc
    21601 taattatgga acacgctgat gcagtacaga ttaaagaaaa agctatttcc aatggaatga
    21661 aaacattgct tcaggatggt gtagaaaaag ctcttcaggg cattacaaca ttagaagaag
    21721 tattacgggt aagctaaacc atgatatttg aatacattgc tctaaccaac aaaggtgaat
    21781 ctgtagcaga cattattgat gcaccatctg aagttattgc acgtcaacgc ttacgagcta
    21841 acggattata tatagttaaa atttcaaagc aggattcgga aatagctgct actcctgaaa
    21901 tttcatctaa tattttcaac acaataattg acagaatatc atactatata agtttacaac
    21961 taagtgcaaa agagatcagc actttttcac gacaattatc gaccatgctt aaagctggca
    22021 tgcctctttt aacatccata tcaacaataa tcgaccagat agaaaaccct atttttaaaa
    22081 gagcaattat tgatattaaa acaaaagttg aggaaggatc atctctgtct aatgcacttg
    22141 aaaggcatca ttatattttc aatgaaatgt atataaatat gattcgtgtt ggtgaaagtt
    22201 taggttcact tgataatgcg cttgaacggt tagctgatct ggaagaaaaa aagaatttat
    22261 taaaaagtaa agttcagtca gctttatggt atcctgcttt tatgataatg tttgccattg
    22321 cagttgtcac tttcctgatg gtaagtatca taccttcatt aggcagaatg tttgcagaat
    22381 taggtaaaga tttaccaata cctactaaaa tagtaatggg tatcagtaat tttattactt
    22441 cattctggta tgttattatt ataattatta tagtaggtat atactatttt aaccgataca
    22501 aaaaaacaga cgaaggaaga agaaagatag atgaattaaa gttaaaatta ccagtagtaa
    22561 gctcattgta taaaaagata ttaatccata gatttaccta taatttaggg attctgttat
    22621 ccaatagagt tgatgtaata aaatcatttg agatagttca gcgcttaatg cgaaacgttc
    22681 ttatagaaga aaatataaaa aaagcagcca aaatggtaca ggaagggtct tcaatttcaa
    22741 atgcattatc aaaagaaagg tttttgccac aatacatcat tggtatgatt gctgcaggcg
    22801 aatcaagcga tactgtagat gagatgttgc tgaatattgg gaaaatatat gaaaatgaga
    22861 tcgaattatc tattaatagc atgacaagcc tcatagaacc aattattatt gtctgtatgg
    22921 gatttgttat tggcttgata gtaatatctg tactattgcc aatttttgaa atgaatttaa
    22981 tagttaaata acgtataata caaagttttt aagggggatt aatatgaagt tacagagttt
    23041 gcttatactt attaaaagaa tttcaaaata tcttaacagc gataaaggct tcacgcttat
    23101 agaattgtta attgtcatca caataattgc catactatca attatcgttg tccccaggtt
    23161 tatggatata cctcagaaag caaaggttga agctgcacgt cagcagatag ctgcattttc
    23221 aatggctctg gaccactatt atctggataa tggtaactat cctacaaccg aacaggggct
    23281 tcaggcactc attgaaaaac caacaaccga ccccatccca cagaattaca atccaaaagg
    23341 ttacatggat aaaaagaaaa ttcccaatga cccatggggt aatccttatg tttataattg
    23401 tccggggcag catggaaatg actatgagat aatgagctat ggtgctgatg gcaaagaagg
    23461 tggaagtgga tttgattctg acataacaag ctggcaatga acaatcgttt atgtatgata
    23521 aaatgcctga aaaccagtaa caatggtttt acattaattg aactgatagt agttattgca
    23581 gttttttcca tattatctat tgttgtaatg ccacgcatta ttaattatct ttcatcagca
    23641 cgagataatt ttttaatttt aagtacaata gttactaaat catttgatga tgcatttatc
    23701 aacaatcgta ccaattttat cgtattccat tgtcaaagca aagaaaatga gatttcagag
    23761 atttcaaatc cactgtttga gcgtacaaat ggtatatcgg tagcaattct taatgaaaat
    23821 tccgaattta tagataatcc aaataaatta ttaaaaaaca gagaattccc ggattcattt
    23881 gccataaaag aaattctttt accagatggc tcaagttata cttccggtaa tgttatgata
    23941 ccagtttatt catcagggtc aaccagtgga gctattgtcc atataataac aagtaatgag
    24001 gaagaatggt cgttgattat taatccatat ataaaagttc ctgaagtagt taaagggtat
    24061 actacgtatg aagatatcaa ataatgggtt taccctgata gaaattcttg ttgcaatagc
    24121 aatattatct atcgccatgt taggtattat gagcgcagtg tcatccggga tatttggaat
    24181 tactaaaaac aaaaacttaa cgatagcaat gctcatagcc caaagccaga tgaataaatt
    24241 tttgatagat aaccaacgtg gtcctgattt gcaggatgag cctgttccgg attatccagg
    24301 cttcagcttt tcacgaacta tcacccggta tgaaaatgct atgattgggc ctatagatgc
    24361 atataaggtt actatcactg ttaaatggtt ggaacgacaa caggaacgct cctatagcat
    24421 atcatatata tacccaaaac gataatatgg aattccaaga ttgtttacat gtaaataaaa
    24481 aaggttttac attgattgaa attctggtgg caacagcagt atcatcgatc attctgctaa
    24541 tggtttatac tatacatcac tcaattattc aatcagtgaa cagtttatca aaaactgctc
    24601 ttttctacga agaagttaat ttagctttat atagaatgaa ccgcgatttt tcctgtgctt
    24661 attttgataa atataatgaa aaactttttt ttataggtac taatgaattt ggtgataaaa
    24721 atttaggtaa agttgatttt gttactatca atgcagtgga tacgttaata gcaggtaaaa
    24781 tgtccgagtc aaccccgctt actgatgttt ttgaagtagg atattattta aaacaaatgg
    24841 aacaacctga tcagaacggc aactatctct actatctaat gaggcgatct gatattatct
    24901 atgatgaaga ccctcttaat ggaggcgata gttcatatat attgtctaat gttgttgatt
    24961 gcaaatttga atttgggata gctcaaaaat tcactgataa atgggattcc cgtgaatatc
    25021 gtcgattccc aaattctgtg aaaacaacat taatagtcaa aaatccaaat ggaaatgatg
    25081 aaaaatttgt atttatatca tatattaatt taagcagata gttactcata accatgttta
    25141 tactgataaa aaaatctatt aagaaacttt ttcaaaagca aaccaatcat ggttatgttt
    25201 tgataattgt actaattatt attgcagcta tcatatcgat ttcatcagaa tttttaattt
    25261 tagctcaaac aaatacaaga tacttacaat catttactaa caggcaaaaa gcttattgga
    25321 ttgcgcgttc gggaattaat ttagctatag aattgcttga agctgataaa agaggttcaa
    25381 ttgcaaactt tttaagtggt gatattgaaa cagaccctaa tgttgattca tataaggata
    25441 tttgggcttt acctttgcct gaaatcccac tggaaaatgg tagcattcaa ctgttaattg
    25501 aagatgaaaa ttcaaaaata aatgtaagtg ttttagctaa tgaatttgtt gaccagacac
    25561 cttattatgt tataacacaa cgatttttta ttaatatggg cttgcccata gaccttgctg
    25621 attgcatttt agactgggtg gatattgatg atgcccgttc tccctatggt gcagaatctt
    25681 cagattacta tcaaaattta aaaaagccat ataaagccaa aaatgctgct ttagatacaa
    25741 tcaatgaatt gcttctcatt aaaggcattt cacctctaat attctatggt ttaggtggag
    25801 gtaattatgg attggaaggt aatcttgtag aaaataacaa aggattacag aatgtaattg
    25861 aatcattaac ctctggtaca aaaatagaaa tttctaagga tacttcactc attaaaatag
    25921 gcaaagaaaa aaacagagcg ttatataact attttagagc taatggtgag cgcagtgatt
    25981 atttgaatga tatcaataaa attaatataa atactgcaag tttcagagta ttatctgcat
    26041 tgacagacgc catgacagat gataaagtga cagaaattat tagaagaaga ttgcaaaaac
    26101 catttaaaaa tgttgatgaa atcagtgata ttattactga tgaaactata cgaaaaaacc
    26161 tgttaactgt tcgttcatat attttcaaaa taaaatctat tggtaaggtg ggttcaactt
    26221 ctttaagcat tgttgcttat taccacagag aacgcaaaca aattataaac tggtcagaag
    26281 aataggactg gaggtttacc ttgtttgaaa atattgctgt tttagatata ggttcattct
    26341 caataaaatg catcaaagca aaaactggtt tcaaaaattt ccaggttgaa tctcttattg
    26401 ttgaggatct tgatttttca attgataata agctggaaag aataactagt ggaattgata
    26461 agatactttc acaaaatgat ttaacagatt atacagttat ttcaacatta tcttcaaata
    26521 aattattttt ttatactttg aattttcctt tcaagaacat cagacagata ggagaagtaa
    26581 ttggatacga aattgcagat tccataccac ttgatatgga ttcagtgata tatgatttcc
    26641 agccaattca aaccaaaagt cctgaaggga tggatgttat tgcagttgtt gctcaaaaaa
    26701 ctttaatcca tcaaataaca gaaatttttg ctaaatataa tctgaatctt gcatttattg
    26761 gagttgagtt taattcacta tttcaatcgt ataattattt caacactgta caaaatgaat
    26821 ctattattca actcaatatt gggtatgaaa aatccattat caatttaatt attaacaata
    26881 gcttatgtgc aacgcgttgt attttatttg gagttaagaa tattgaagat cattttaata
    26941 agatactaac tgaacattat ggcatacacc aattcattgt aaatcattct tttgatctct
    27001 catcttttga aaataatata cattatggct tacataaaaa actaaacatt tcacagcaaa
    27061 aatttaaaac aatatttaat gatatccaga atgaaattga taatctcatc aatgaaattt
    27121 tgctatttat taatgcagaa actcaaaaat atgaaaattt ctcattacaa agaattatta
    27181 tatccggcgg aggggcatta cttacaggtt ttagtacagt tctatcccaa caattgcaaa
    27241 taccagttgt attgcaaact ttgatggacc aaactggtaa tactgaggtc caatcacaat
    27301 tctacattac ttttggtact ttaattgatt atatcaatcg ttccaaacaa aaaactatag
    27361 atcttcttaa ggatgatttt caattatctc tatctcaaca gtctaaaaag aatttttata
    27421 ttgccctgtt ttatggttca ctaagtatac tctttcttgt tttattcata atattatcct
    27481 tgatctacca attatcatca tcatcgtatt acaacaggat tcttgaagat agatataagc
    27541 ggtattttct taccaatatt gtgcctgatg accccattaa agaagcaaca aacagatatg
    27601 caacattaaa acgcgagata gatagcatag aatcttttct aaaaattgat gataaaatga
    27661 tagaaattct caatttatta gtttctaatt ttccttatga cgcaaatttt gatttaaata
    27721 atatggttat taatgaaagc atcatcaggt tggatggatc aatcaattca actgcaaaga
    27781 ttgatgaatt taaaaacagg ttagtacaga cacaaaaatt tgaatcggtt gttttcaata
    27841 ccaatgttat gcaaaacaat gtaaaatttt caatgacaat taaattcaaa tcaaataagc
    27901 ctaaacgtca aactactgcc aatgaggatt aattatgata aagctttcta acagagaaaa
    27961 attattatta aaaattctta tcgggatatt aatacttatt attggatata attttatttt
    28021 cacgccatta attaattttt tttcatcttc agatacatta attgtcagtg ataaacaaaa
    28081 aatagaacag cttgatagaa tatatagtaa ttataaagaa ctggaaacaa aacgaaatca
    28141 atatatgtct cttctgaaaa gtgattcaga aaatataagt agcatgattg aacaatgggc
    28201 tcaaagcagc aatgtagcaa agaacattgc ctactcacgg cgcaatcaaa caaatgttca
    28261 gaataaatat attagaatta caaccgacat gaaattagat gcagtatcaa ttcaatcatt
    28321 acttagattt atttatgaga ttgaaaattc gaacaaactg gttaatatca gctatctcag
    28381 aattaataaa tcatataaat cacctaatct gtatgatgtg aatttaaaaa ttgaaagctt
    28441 tactttacaa taatctcctg gatgcatgat atgaatctaa gtaattataa aataatatta
    28501 aaaacatatt ttgacagact ttataatttt cttaaagaga tagttactgt acaatatttc
    28561 tggcgttatt tagtgttttc aattgttatg gtaatactat ttgttttttt aacttttccg
    28621 tatgaaatta ttattcagaa taaaattaag gaagttgaac agaatattgc taattatatt
    28681 tctataggca ggttgaaaat tcatactatc accgactcta tagctgaaga tataacaata
    28741 gaattaaaaa atggcacgga gattactctc cagaatgtct tgtttaactt tactatcaat
    28801 ccattttcaa tatttattac tcaccattat gatggaacaa taaagataca taatctgaat
    28861 cttaaaggtg agaaattaca gataataaca aacgctgata ttctctatga ccttaaatat
    28921 aacaaaccgc ttccatatcc agcagagggc aatattattt caagcatgaa ggatgtttct
    28981 attaaaggaa tttctatcca gggttttgaa atacccgata taaatatttc gcaaattaag
    29041 tcagaactga tttttgaaaa gggcaatatg attaatctta aatatctttt gttctcaggt
    29101 tcagagatac gaggcaatgc gtatggaaca attaccttat ccaattactt ccagaactct
    29161 tctcttaata taacaattca aattgactca caatcaagta ttctggaaga ttataaagtt
    29221 ttgttagaat cgatgatgaa agctggtaca aaaaatatca caattaaaat aatgggtaca
    29281 ataggcaatc ctaattttgt tactcaataa aatttaatat atgaatatta tatgaaaatt
    29341 aaaattgctg aaaatactgg cttttgcatg ggtgtgcgta aagctgtatt ggatattgtt
    29401 gatgtaatca ataaaaatag tgaaacgatt tatgttatag gtcctcttat acataaccct
    29461 caaacagttt ccatacttca atcaaggggc attatcacaa ttaattccat tgataatatt
    29521 gaaaataagg taatagctat ccgtacgcat ggtattccca aggagtcatt acggttatta
    29581 aaacaaaaag cctccaaaat cattaattta acatgccctc gggtagcacg tgttcagggt
    29641 atagtcaaaa aatattccag agagggttat tttgctatta taataggaga tgatcatcat
    29701 gcagaagttg aaagtgtaaa aagttatgct gatgccggtg ttatagtaat atcaaacatt
    29761 gaagatatca aaaaaattcc tgatgcttta aaatatatag ttgtagcaca aactacattt
    29821 gatgagtgcc tttttgaaga aattactcaa attttaaaaa gaaaatataa tgatatactg
    29881 atttttaata caatttgtga ctctactcat aaaagacaaa gtgatgtgat taatgctgta
    29941 aaagaaggaa ttgatacatt agtagttgtt ggaggtaaaa attcagctaa tacccagaga
    30001 ttggcacaaa taggtaaaaa caataatatc aaaacattcc atattgaatc agaagaggag
    30061 ttgaatgatg aatattttaa aaattctaat tatgttcttg taacagctgg tgcttcaact
    30121 cccggatgga ttatcaataa tgtacttgag aaattatatt ctatatcaaa taaatataaa
    30181 aatccattat tgtattttat aatgcatttt tttgaaattg caattagatt aaatctcatt
    30241 tcctctatag ccagtttttt catgtcactt ttaatagaaa aattatccgg tgtatggtca
    30301 tcatataaac taccatttct ttccgcattt tttatattta taatgtatgc cataaataac
    30361 tactttttaa aagatactct taaaatcaca aatccattta aatataaact tcacaaaaac
    30421 aatcatttca taattttaac ttccactatt gcagccattt tgttttcggt tatgattaca
    30481 ttgaatatta aaaattattt atccctactt gtgtattata gtatattact gacaggaatg
    30541 gtttattctt ctagctattt caaattcttg attaataaat acacatcttc cttaataaaa
    30601 acaatttatt ctattaaatc aatccctgct gacattggtt ggattattgt tacgttattt
    30661 ataccagttt gctatgcttt ccaagattcg aaattgagtg agatacatac aggtattgtt
    30721 ttatcattaa taatatttct atcatatttg atagtatcaa gaaatttatt gctggatttt
    30781 attggtttca atggtgatct cattattgga aacgttacgg tgccaataat tttaggaatt
    30841 aataaggcca gatatttatt ctacatatta tcatgtatgg caatgatatc attattctat
    30901 ttatctatct tctacaacat aaagtttata ttttttatta ttaatataat gatcaattac
    30961 taccttgttt taaaaatttt aaacctcaaa tattttattt cattaaaata tgaaatgcta
    31021 acagacatga accttgttat ttttatttta ttatgccttt ttattattat tggttaaccg
    31081 ttttcatttc agataataat tcaagatatt attattttat aaattggaga tacattaatc
    31141 tagtcaatat tttcattttc attatatatt acaaattcct tttctgcaat ggtattccct
    31201 ttatctacaa ttaatatctt atatgtacct gcttcataat tttcaatgtt tccatatatg
    31261 caaggcttat ttttgtaaac cgtatcataa aaagaataga catcaaccca gactttctct
    31321 ttcttcaata caactactcc aatttttttt tgctttgata cttttgtaaa gcaaaaagcc
    31381 caatttactt tttgatcctg gcgaaattca cacaggccac tcttaattat gtacctgtca
    31441 tcattgttat tcttaaacgc aaatgtacct gtttcttttg aacatgaaac tacgaaaaca
    31501 aaataaaata ataatatgat atatttactc gattttgtaa tcatctttgt ccagttctat
    31561 tactttattt ttagtatctt gataattata ctctttcttt ttatttatat cttcagattg
    31621 ctttgtactg atttttataa tatatttaac aacatgtatg aagaaatata ttataaaaaa
    31681 gaaaaaaata attcttatga gattacctat catattgacc ttgtttataa gttttattat
    31741 tcacaaaaat tcttattttc tactgctact tcataacttc atttgtcaac ttataaattt
    31801 caataatcat cagttattct cgtttataat tcgcaccatt tcactgactg cattggccaa
    31861 ccctgtgaaa atagaacgtg aaataatcga atgccctata tttaattctt ctaatccagg
    31921 cattgaaaga attggtgctg tatttatata atttaacccg tgacctgcat tgactcttat
    31981 accaatattt gatgcaatgg tagctgcata aaaaagtcta cttttttctt tatcaatagt
    32041 atctttcccc tttgcattac aatatgtacc tgtatgcaac tcaataaaat ctgcacaaga
    32101 ttcttttgaa agtttaacag attcttcttc tggttctata aacagggaaa ctaaaatgtt
    32161 ttctttgtga aataaggata ctatttcaca aatcttttta aaatttgttt ttacatcaag
    32221 cccaccttcg gtagtaattt cctgcctttt ttcagggacc agggttatct ggtcaggttt
    32281 aactttaagt gcaatttcaa ttatttcatc agataatgcc atttccaggt taaatttgcc
    32341 tttaactact tttcgtaatg caaaaacatc atggtcctgt atatgcctcc tatcttcccg
    32401 caaatgtact gttataccag ctgcaccatg ttcttcacat ataattgcac cctgtactgg
    32461 atcaggttct ttaccacctc ttgcctgacg taaagttgct atatgatcaa cattaacaca
    32521 taattgaacg tgcgacatta tcaaagacct ccaatattac tattcttcat gtgatatata
    32581 tacttctgtt tctgtaggtt cataggtgaa tatttcaaaa ggtgctttta cctttttttc
    32641 aacaaaaatt ggtaatttta taacttcctg atcattggca tcaatcacac catttacata
    32701 ggcaaccagt acattttcat ttaattcaat attcatcaaa gactttttta tatacaccac
    32761 tactttctta tgtttcaaaa tatatttaaa atcattgcgc tgattacgta tttcaatggg
    32821 cattgttaaa cttacaagat tagatactga agtaattggt attgtaagca ttacagtatc
    32881 aggagaaata aaaatctttg aataatcctt tatattgacg tgtacaggaa catgcaatgt
    32941 ttgaatttct ttgtctatgt tcaacggata cgtctttaaa ctttttatag catctacaat
    33001 aatatctgca ccagatattt gaattaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33121 nnnnnnngtt ataaatctcc tctttgcttt tgggattcat gtaaaataat atcatattct
    33181 ttaaatctgt atattttatc tttgattgaa atttgtcatt taccataata gaaatatttc
    33241 ctgtttcctc agatgtaact ataaccagcg catctgtttc ttcagccatt cccaatgctg
    33301 ccctatgccg cgcaccatgt tgttttttca actgccgtga atcactcaat ggcaaataac
    33361 aagctgctgc tgcaatcttt gcttctttaa taattatcgc cccatcatgt agcggagtat
    33421 ttgggaaaaa aattgtacgg attaattcct ctgttatccc tgcattaacc acaactcctg
    33481 actcaatgta tccacgaaga tctgtacctc gttctataac aataagtgaa ccaaccttct
    33541 cttctgacat tgcaataatt gcattaacaa gttcatctaa tggaaatgca tcagtattca
    33601 ttgatgttgg caaccatgtt ctttgaccaa attgtgttat aattctgcgt aactctggct
    33661 gaaagagtat gattatcata atcacaatgt atgatgtcaa atttgtaatc agccagttta
    33721 acgtttccag tcgcaatact tttgataata ccgcagctat aaaaatgata atcaggcctt
    33781 ttaaaatctg aattgcccgt gatctagata aaaatgtata aatccagtaa aagatatatg
    33841 ctacgattaa tatatctatg atgattctaa catattccca gcttatttta atatagtccc
    33901 aggctgttgt gaaaaaagga ttcatgaatt tgcaacctga tataacattt ttaaaggctt
    33961 aataataagt ttatgctctt taacatcatg cacacggata atatctgcac cattatacat
    34021 tgcgatagca tttaatgcta tagttgctgg caatctatct tcatgggtat catacaactt
    34081 tccaatcaat gactttcgcg acaatccaat taagacagga taccccaact ttttaaaata
    34141 ttgaatatta ttcagtataa cgtaattttg ctctaatgac ttgccaaatc caatgccggg
    34201 atcaactata attttatcat cggcaatatg cgctttttta gcaatctcaa ttgattggca
    34261 taaatatcgg gcaatatctg ataataaatc attatattct gtatttaatt gcatagtctg
    34321 aggagtaccc ttgatatgca ttataacaca ataggcatta tagcgggcga tagtaactgg
    34381 catatcagga tcaccctgta atccggatat atcattcacc atgattgcac caagtctcaa
    34441 ggcttcatca gcaaccctgg attttcgggt atcgatagaa actggtatat taaattcatg
    34501 tatgattttt tcaattacag gacacaccct gtcaatttcc tcttccagtg taacttcttc
    34561 agcaccaggt cgtgttgatt ccccaccaac atcaataata tcaacgccat cattaatcat
    34621 ttcccttaca tgatataatg ctgcatcaat tgaattatat ttacctccat cataaaatga
    34681 atcaggagta acattaagta tacccataac aagtggacca tgtttgatat taatatctat
    34741 cctgttcata gtacaagcat cactgaaata aaattgttat aacccaaatc aaccgaatcg
    34801 tatgaaaaag cataacctat agtacaatca cctaatccta ttgtgcatcc agttgtaaat
    34861 aatgaatcat ttaaacccaa ataaactgac atacttttat atacaagtaa ttcaaatcct
    34921 atattatatt cataatcatc ctgttcaatc tttttaatcc ctgaaaaagc aatagtaaaa
    34981 ttatt
//
LOCUS       DAORYL010000023        34883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1002_length_34883_cov_13.607701, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000023 DAORYL010000000
VERSION     DAORYL010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 34883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34883
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1002_length_34883_cov_13.607701"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PLV81_06210"
     CDS             <1..552
                     /locus_tag="PLV81_06210"
                     /inference="COORDINATES: protein motif:HMM:NF019944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HOM09731.1"
                     /translation="VAIAIDGYQDLPRLPDGSYDLDPTKKPIQVPLTEDELRQAENIV
                     KKAINFNPARGDQVAVENIMFDRTKQWEQLREEFRKKEQFKRMLLAALVGILALFLGV
                     VLFRAISRELARRRRIREEQLALEQQRMREAALRAAEEEGIDVELSLEEKARLELQQN
                     AINLARERPDDVAQLLRTWLAEE"
     gene            701..1717
                     /gene="fliG"
                     /locus_tag="PLV81_06215"
     CDS             701..1717
                     /gene="fliG"
                     /locus_tag="PLV81_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOM09732.1"
                     /translation="MLQQKKSQLTGRQKAAIFLVSLGSDVSSEIFKHLREDEIEQLTF
                     EIARLDKVEPEDRDKVLMEFQELMMAQEFIATGGIDYAREVLERALGTQKAIDIVNRL
                     TSSLQVRPFDFIRRTDPSHLLNFIQGEHPQTIALILAYLDPQKAATILSGLSHQIQAD
                     VARRIATMDRTSPDVLREVERVLERKLSTLASEDYTSAGGIDAIVEVLTHVDRGTEKI
                     IIEALEEEDPELAEEIKKRMFVFEDIVLLDDRAIQKVLREVDTQDLAKALKGVDAEVQ
                     EKIFRNMSKRASALLREDMDFMGPIRLRDVEEAQQKIVNIIRKLEEAGDIVVARAGEE
                     ELVV"
     gene            1710..2636
                     /gene="fliH"
                     /locus_tag="PLV81_06220"
     CDS             1710..2636
                     /gene="fliH"
                     /locus_tag="PLV81_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HOM09733.1"
                     /translation="MSKLVFKPTEIQVISAPKQIELPEKYQKNVLTDEEYAEFEVDEE
                     GNPVEVYQGPSIEEMEAELERYRRETEEEVRRMLEDAKAKAAQIEEEGKKAAFLELQQ
                     AREQIKIEMERFKIESEREIERGKFEAEKMIKEAELKVSEIEHEAYKRGYDAGREEGY
                     KEGQAEVMRLIDRLGTIVSTAVDIRDDIIRSSEKLMTEMILMIARKVIKDEIVERREV
                     VINNIKEALQRVKDRDRIDIRVNFADLDMTTAHKDELIKMMESLKKVNIYEDSRVERG
                     GCIIETDVGAIDARISTQLESIEEAIRNARPI"
     gene            2658..4067
                     /locus_tag="PLV81_06225"
     CDS             2658..4067
                     /locus_tag="PLV81_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HOM09734.1"
                     /translation="MIQILPHEKVDIFSKYKDVLEEVDTLRATGRVEKITGLTIESHG
                     PDVQYGELCRIRLKSGNYLYAEVVGFNKNKIILMPIGDMKGVVPGADVYAVGSQLFVP
                     VGEALIGRVIDGLGNPIDGKSKLFLKERYPVDNKPLTPLEREIIDSPLPTGIRAIDGL
                     ITVGKGQRIGIFSGSGVGKSTLISMIARNTAADISVIALIGERGREAKDFVEKELQNE
                     GLKRSVVIISTSDQPPMVRLRAGFLAHSVAEYFRDKGLHVNLLMDSITRFAMAQREVG
                     LAAGQPAATRGYPPSVFSLLPQLLERAGNKKGAGSITGFYSILVEADDMNEPIADAVR
                     GILDGHVVLDRKLAHKNHYPAIDVLGSISRCMNDVIDEEHKKAANTFRKLLAAYREAE
                     DLIMLGAYARGSDPDVDKAIDMKPAMDAFLQQGIYEKDTYENIKNKLSELMLGTKAKP
                     SKRQEFVDRTPYFAQSRGR"
     gene            4084..4527
                     /gene="fliJ"
                     /locus_tag="PLV81_06230"
     CDS             4084..4527
                     /gene="fliJ"
                     /locus_tag="PLV81_06230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HOM09735.1"
                     /translation="MKKFEFRLQTLLDLRISKEHAIKAELARLIAKQNEEIALQQNYK
                     RRVTEYYARLSANMRQGTFSYSEALMCERFAYNAEKAIALSQNRVEQMQPEIEKVRQR
                     LIQASKERKVVERLKEKKYEEYMYQYNREMGKELDDLNQRMYNNA"
     gene            4538..4885
                     /locus_tag="PLV81_06235"
     CDS             4538..4885
                     /locus_tag="PLV81_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09736.1"
                     /translation="MDINSIKQVQDRIKEIKNRFKTIQNTYAKVSPSTSMVMADSKVA
                     EASKVTATVQENSEPQKNNTTVKMTEEILKNVLNSKKESTKETLDVQQIQNLLQKAIT
                     QYTSNGKIFNIEE"
     gene            4920..5519
                     /locus_tag="PLV81_06240"
     CDS             4920..5519
                     /locus_tag="PLV81_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09737.1"
                     /translation="MKLVSDSTKVIYLAIMIVFIIIVGLFWLDYIGLINIGKMYHQYI
                     AKEAPSVVDAADDEPSLIEREEFKKEKDKLLQRIEDLDKREAKIVEAEKIIAKEKEKL
                     EQLQKGLDQEKKALELEKKKYSGYKKNVIDLAKKIESIPPQDAVDIMVQWEETLIIDV
                     LRQIDQNAAKEGRMSISTYLLSLMPKEKASRVMYLMTQL"
     gene            complement(5523..6104)
                     /gene="yihA"
                     /locus_tag="PLV81_06245"
     CDS             complement(5523..6104)
                     /gene="yihA"
                     /locus_tag="PLV81_06245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOM09738.1"
                     /translation="MKINTIRFVTSAHIPSQYPKYTLPEFAFTGRSNVGKSSLINTLL
                     GRKNIAKTGSKPGVTQTINFFCINEKMSFADCPGFGYAKVSHNKRLEFIEMIHSYIIS
                     RPNLKLLFLLIDIRREPDENEQAIITKALNNNINVMILATKCDKVSKNEINNGISRIC
                     TSLNISKEYVVPTSAKTGLGKNIILAMIQEYCK"
     gene            complement(6094..6546)
                     /gene="rimI"
                     /locus_tag="PLV81_06250"
     CDS             complement(6094..6546)
                     /gene="rimI"
                     /locus_tag="PLV81_06250"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOM09739.1"
                     /translation="MPNKKILIRKALYEDIENIAALDAYVFRENAWSTESFTRELDLH
                     FSRLFIAEIDNKFAGYALAWYIADEVQLMRIAVLPHFQRYKVGTQLIQHVINHTDNQK
                     KIVLEVADTNTSAIRFYTSLGFHSVGFRKNYYRFDNAILMEKNINHED"
     gene            complement(6551..7339)
                     /locus_tag="PLV81_06255"
     CDS             complement(6551..7339)
                     /locus_tag="PLV81_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOM09740.1"
                     /translation="MGDSTQKKCHYTVENGIALLTIDNPPMNALNHVVLKDMRETIEQ
                     LIENDSVRVVIVTGAGKAFVAGADINEIKEQNTREDGANFLKNGQDVLNIIENADKPF
                     IAAINGYCLGGGLELALACHIRLADESAQLGLPEIKLGIIPGYGGTQRSTRLFGKGAA
                     YELILSGNFISGKQAELYRIVNRATEKGKVVDEAKELAKAIAARSRLAIKAAMQAIAK
                     GLTMEFYSALTLERELFGQLCETEDKKEGISAFLEKREPKFKDK"
     gene            complement(7419..8327)
                     /locus_tag="PLV81_06260"
     CDS             complement(7419..8327)
                     /locus_tag="PLV81_06260"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOM09741.1"
                     /translation="MNNKPIIGIVGAGAMGSLFAFFLGKYTILLYDTNPNTVNAIKQG
                     LSVEIHSEKQTFYPTISNDPAILASADIIFLFIKSFATRDAIDTIQHYIKPDAIVVTL
                     QNGIGNVELISSMINNPLVYGTTTIGATKLSPASVRFGGTGSIIIGGDSYSHCSTVHS
                     LLQNANLTVTIAPDPKKALWEKAIINAAINPLGALLEVPNGKLIENNNTVKIMAGIIQ
                     EACLVAQANGIMTDSNHMIETTTTVCRNTQNNFCSMLQDILAKRKTEIDSINGAIITF
                     AQQYSIDTPYNTTVYKLIKYKESQLI"
     gene            8575..10041
                     /locus_tag="PLV81_06265"
     CDS             8575..10041
                     /locus_tag="PLV81_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM09742.1"
                     /translation="MDNPVSLEYTTLLNTIQAPVFVVDASKNIIDINNYAKRLFHSLD
                     VIGKSCKDIFPECQKSCTCPVDEADYKIPTDSQRCKQVMVLNTVKEEVELCIIPVQTA
                     HDIVFLHILTDNDIQNRQNLVMLEKNMTISTLASGIAHEFNNMNAGIQGIVELLLSQE
                     ELSSAGVKDLKTILKIVKRATHLIDQLSMLAHRKPSKQMLVNIEDIVDDCISIIKPQF
                     MDAGITIDVIHKDRLPELFLDANKITLALMNIFLNARDAMIDTEQKKLAIITGQDDTY
                     VTITIKDSGCGIKEEHIQRIFEPFFTTKGPLGHSNIPGTGLGLSVALGIIEEHGGTLA
                     VESTVGEGTTFTIQLPVNTDDKVASEVAINYTEFDFSGKKILIVEDDPEFATILQRAL
                     IAKNAEVVYAGNGAEGLYYLDNQTVDLALLDVHLPDMTVWDILKKVQYMNNRPNIIIV
                     SGNYLAMNNNYIGIVDYVLLKPFDLEELFTAIKSSLTI"
     gene            10124..11056
                     /locus_tag="PLV81_06270"
     CDS             10124..11056
                     /locus_tag="PLV81_06270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOM09743.1"
                     /translation="MTKKDTVLLIVNPIAGRGKALRSVERIENDFKTLNIKHDVVLTE
                     RVWHAAELAEDAAKKGYRAVVVASGDGTANEVLNGLMLARKKGYNKTAMGQIPVGTGN
                     DFAYGMGLPNDIDECIKIIADFNVRRIDVGFIKGGDYPEGRYFGNGIGIGFDATTGFV
                     ASRIRFLSGMMVYLLAALQTIFIYYKAPRIRMIYNSKETIMDALQVSIMNGKRMGGGF
                     HFAPDGSPFDGLLDLCMVRSMNQFKIFGMIPYFIRGTQARRKEVMQDRTTKIEVVAEK
                     GSLPVHCDGETICYSGKELFIQLFPAALDFITKA"
     gene            11151..12095
                     /locus_tag="PLV81_06275"
     CDS             11151..12095
                     /locus_tag="PLV81_06275"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: protein motif:HMM:NF013446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HOM09744.1"
                     /translation="MNVTSIHKLILVFLSIIILISIYSISYAQTAYLETNTGAGFSQG
                     TIGEYVYDNNRMLSRLNWNIDYLWYYHFSVALWYNSFFAGIQVHNGINDKIGTIDDSD
                     WTDDNNPSLKTHYSKHDNYLERYASYDCNIGFKSDITDTINVKPYIGFLYKSIKMSAR
                     DGYLEYPPGSPEDPVYGVGIIYEQRYYIPYIALASEIIVTETIGIMAGISVSLFAVAE
                     DIDNHVHRNPGDDIDFYANYDGIIYLHAYGGCFFSLSDTIKVALDLLYEYVPEQKGDT
                     YYINTSTGIKSPVFNNSAGLKYSVFSVQLSVVYTLKLL"
     gene            12441..12863
                     /locus_tag="PLV81_06280"
     CDS             12441..12863
                     /locus_tag="PLV81_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HOM09745.1"
                     /translation="MDRIQYRKLNKYKYQLMRDYVYKTTITLDHDAGVPDFVTLRKDG
                     QLEIYKGYAWDGPSGPTIDTKDFMRGSLVHDALYQLIREEKIERKYKEYADRLLSDIC
                     REDGMSKFRAWYVYWAVRLFGKARKNPDDTSNKLEEAP"
     gene            complement(12867..13208)
                     /locus_tag="PLV81_06285"
     CDS             complement(12867..13208)
                     /locus_tag="PLV81_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09746.1"
                     /translation="MRQLLFIVILCSIVFLGCVTTHHVAGYEPHVRPLKDISYQIVGE
                     AESFTSNFNLLWFFNVTPLASFDRAISDAINQKNGDDLIDVSWYIERQYWIVGTVNII
                     HIKGKVIKYTE"
     gene            complement(13211..13579)
                     /locus_tag="PLV81_06290"
     CDS             complement(13211..13579)
                     /locus_tag="PLV81_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09747.1"
                     /translation="MKNIVPLIIMITIVFLTGCVTPMGMTASSTPLHNRIIVENLGQA
                     EGSDWSWSLFGLWSVGRPDMDRAIHEAIASKNGDALINVRWYEKHYYFVLFSITKVIV
                     TGQVIKTAPVETKEQVKQKR"
     gene            complement(13644..14024)
                     /locus_tag="PLV81_06295"
     CDS             complement(13644..14024)
                     /locus_tag="PLV81_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09748.1"
                     /translation="MVIGYTVGKHTISARFQAYSGFPYTPVIDSDESPDNSGRYVPIY
                     DYDHLHSKRFPWEHRLDVRYSHKTNHEWGYVSWYIEVINIYNYKPKNDLAWNYYKPHG
                     EDNPKLKTQEGLNLIPNFGVEVKF"
     gene            complement(14309..16030)
                     /locus_tag="PLV81_06300"
     CDS             complement(14309..16030)
                     /locus_tag="PLV81_06300"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOM09749.1"
                     /translation="MSISKKLILIWAIVTIVSTIALGQNQYAIQGTIYSQATKKPVDF
                     ATIAIPEIKQKFYTKADGSYSILFEKPGTYTLIISSLGFKPVTVTVAINGIMQKDIVL
                     QPIAIKGAALTITGERDIQKVSRYTLTAQDLKAVPASFGDSLNALTSLPGVIRTSGFF
                     GPLVIRGADPSFNGYFIDDIPVYNPQHFGAIHSIINNDLMSEIDLYSSSFPSPYANAI
                     GAVISINTIDTVKEFGGSADVGLISSNLLLKSPVTEYNEATNQEETRGYWIVSGRYAY
                     LSLFVPAIYKLLTGDEIESVPEYWDYQFKGKYYFTKTQSITFLFFGAYDYIKFINEST
                     PPEEVDPLLASFEFKNNLGSHALGVYYRWQPVSRFENTIVMFGSLTNSYNYLNIPSVD
                     AADWVKDLHITSKPYIYGAKEKIKLEWWKDHAQFRGALEYTLYHFTNDGYTLVLNAPL
                     YNIPDFGDPDLATRVPLGQKFNNHVLGAYAEQKFIFGGLTLLPGIRTDYLTRTKQQTV
                     DPRLMVSFEFPSQTTVSYATGKYSGFIQTNSNLFNFQPNLAGAGDEYKPQSAYHNAIS
                     IEQKLSL"
     gene            complement(16034..16663)
                     /locus_tag="PLV81_06305"
     CDS             complement(16034..16663)
                     /locus_tag="PLV81_06305"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOM09750.1"
                     /translation="MPFRQTLDILLQHVSQFQQQHPYIVSFIISSCIAAFTLFFTAPI
                     YVADMPEVADSLEFINIETVTAQSKRIATKEISTTTGEATPSSTDRAVGLSNESEVFD
                     ISFYPNIVPPRPIGALKKIYPQSAREKNVEANVNVEIVIAPDGKVSTVQILSIRLSKD
                     LPPEQYTVLSKEFSKAAYSILTQARFTPPVINGQRVAIKMELPMQFKLE"
     gene            complement(16663..17067)
                     /locus_tag="PLV81_06310"
     CDS             complement(16663..17067)
                     /locus_tag="PLV81_06310"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOM09751.1"
                     /translation="MVQSPFLKKLRSFSLGASAADMAMLLLVFFMATTSTEPPKGVTV
                     ELPTAVTQGAEQDSIYISISKEGALYFDGKDSSIDDIKDQLAIRQNEKDRIVSITADK
                     DLPYSVVANVINILRQYDFLNVVFISQPREEQ"
     gene            complement(17070..17492)
                     /locus_tag="PLV81_06315"
     CDS             complement(17070..17492)
                     /locus_tag="PLV81_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOM09752.1"
                     /translation="MYWFSRKNKPQQKQSRLIEFDTTPIADLSFNLLIFFIVTASFII
                     RQGVFLSLPSLSAGTVKVEPSQVIEIYPQDNGFIADGKALDRKALLQYLTVRKSQTKE
                     AVSIIYMKPTIKYERLVDTLSAIRESGLSRISLKYNER"
     gene            complement(17492..18136)
                     /locus_tag="PLV81_06320"
     CDS             complement(17492..18136)
                     /locus_tag="PLV81_06320"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOM09753.1"
                     /translation="MNKIVTFIILFLSQPLFAQELSPNSSNLWATFKQGGILMWPILF
                     LGIVGLTIIIERALFYYKIKIWDTAHIENKLNELLNQNIYKFREEAIDDLEKNMQVYY
                     NQLEKGLVLLNAVGNLAPIIGFFGTVQGMIAAFASIAAATTVNAKVVAVGIQIALITT
                     AGGLSIAVPTLAFYHFYVHILQNVYAKASELIIHKTKQLPRYSQIESTTSLEGV"
     gene            18263..19270
                     /locus_tag="PLV81_06325"
     CDS             18263..19270
                     /locus_tag="PLV81_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006194906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HOM09754.1"
                     /translation="MNILITGTAGFIGFYTALRFLQEGHCVIGIDNMNSYYDVSLKEA
                     RNNILKQHDNYIFYLVDIASYQELSNIFKKHSFDYVVHLAAQAGVRYSLDHPFDYVHS
                     NIVGFMNILECCRHNPVKHLVYASSSSVYGLNSCVPFSVHDNVDHPVSLYAATKKSNE
                     LFAHCYSHLYSIPSSGLRFFTVYGPWGRPDMAYFKFTKAILENKTIDVYNFGDMERDF
                     TYIDDIVEGVYRITFKVPQGQPEFDTMHPDPASSSAPFRIYNIGNNKPVQLMYFIEVL
                     EKETGKKANINFLPMQPGDVLATFADIDELTDAVGYRPVTSIEEGLHKFVQWYKEYYK
                     V"
     gene            complement(19322..19840)
                     /locus_tag="PLV81_06330"
     CDS             complement(19322..19840)
                     /locus_tag="PLV81_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09755.1"
                     /translation="MIKKFLQYYIILFLLLQSAWAQLTTTIIEENREFWNTLIQNITI
                     SNKNLLYASYIAYKDKLYDLSIDSFQECINQNTDNQVIQGIANYYIGKNYYNLGEYKK
                     AVLYFLATERYTMDQYTYIKAAALINASVCYIQINDTVMAKTYLQKSMQYDTEGAYKT
                     TIETLLQSLKDN"
     gene            complement(20005..20163)
                     /locus_tag="PLV81_06335"
     CDS             complement(20005..20163)
                     /locus_tag="PLV81_06335"
                     /inference="COORDINATES: protein motif:HMM:NF019057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HOM09756.1"
                     /translation="MYHDKKGEYRFRLKAANGETIGQGEGYSKRSGCMRGIESIKKNA
                     ANAKVVEI"
     gene            complement(20368..20541)
                     /locus_tag="PLV81_06340"
     CDS             complement(20368..20541)
                     /locus_tag="PLV81_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09757.1"
                     /translation="MDTIKQPKQQPNEQQHPETIVSQWNNFKLLESQHIYFSVGKANI
                     KHILIKREPLIGA"
     gene            20803..21498
                     /locus_tag="PLV81_06345"
     CDS             20803..21498
                     /locus_tag="PLV81_06345"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HOM09758.1"
                     /translation="MLSTLNKKLLRIAIAPVIVFLCVITGYSYKFDFVTVNDIVKQIK
                     KTFSDVESYQANFVMVSDKMGKRQTQSGTIWYMSPHYLRIDFDVPYGQKIVSDGKQVW
                     IYIPSINVVAEQDLNSSMFAIGTGSGLNRLFSKYHYKFDGKEQPRVEGNNKYYVLFLK
                     QKETRSGFRTMKLWVNEQYFITKAAGETANGKNITIKIKNINTKVDLKKGFFKFDPPS
                     NARIIKNPMLAEE"
     gene            21502..22635
                     /locus_tag="PLV81_06350"
     CDS             21502..22635
                     /locus_tag="PLV81_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HOM09759.1"
                     /translation="MVAGIGETLRNAREAKRLTLKDVSKDTNIVVKYLEALENEDFDK
                     FPSETYLLGFLRSYAEYLRVDADELIQAYKGYKIGESSTPLEELTRPTGLSIVAQLRM
                     YAQQYRNILYFAGIGIAVLIVISLFISLITSDVHVGSDDSIKAIQEEYYRTQGKKIAI
                     KNIWPLQLTNDSGIVLLYNEEACQFLVDNKEILFLLNDIKDNSVIVEFLPDSKKYVLE
                     MEKPVICSPMESSRNIVLTLKGLTENRAKIRVQLAAVEQEKPDEAVTVTTTVTPPAEV
                     VTHDQKNLKIVFEAEFVQKTYFELYLDGNERVRGMMPAGTKERWEASEFIQIKIGNAG
                     GVKVKINGTDYMFGLPGQVANKVITWRKDPQDPNKYKLEVKDW"
     gene            22637..23947
                     /gene="rimO"
                     /locus_tag="PLV81_06355"
     CDS             22637..23947
                     /gene="rimO"
                     /locus_tag="PLV81_06355"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOM09760.1"
                     /translation="MFSPDDISYYIISLGCPKNEVDSERLNADMHSAGFVEAHEIDTA
                     DIIFINTCGFIEAAKKESIETILDALDYTSQQTKTFKKRLVVLGCLTQRYKQEIIQDI
                     PEIDLVWGLYDSSLLKEIARLFNIALNAGTIAQKRKPLIDNLPYAYIKIAEGCSNFCS
                     YCAIPLIRGRFNAFPPSLIVADAREALNRYVKELIIVAQDTAVYQYDDKRIWDILAMI
                     SNLEGDFWIRLMYCHPDHIDDRLIEALVTVPKVVPYIDIPFQHASKKILQSMNRHGDA
                     DTYLKLIEKLRDAIPDIAIRSTFMVGFPDEDDNDFKLLMDFLQEAQLDRVGVFMYSPE
                     EGTKIFAKGDTVKKATKKNRYDMLMNLQQEISRERLMETLGDTVQVLVEEKVDRYNYI
                     GRTQYDAPEIDGIFYLTAYNVRVNDIVTAIITDTLEYDRIGEMI"
     gene            23944..24597
                     /gene="pgsA"
                     /locus_tag="PLV81_06360"
     CDS             23944..24597
                     /gene="pgsA"
                     /locus_tag="PLV81_06360"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOM09761.1"
                     /translation="MNIPNLLTLSRIILIPLFLYLIFTPTIEHRIWALVIFIIASFTD
                     FLDGWTARKLRQETDTGKFLDPLADKFLVISALIAFLFLDPLIPLWMVIVIIARDLLI
                     TLMRYLSIKKGSMLRTSRLGKFKTAFQMIGIIVIIMVFIVRNSIKNVQLEINQLSALK
                     LETVVEILNSNLVHKWLIVCPYLIMAVVTFLTALSGLRYIITNWRLFIPPYTQKDAK"
     gene            24604..25083
                     /locus_tag="PLV81_06365"
     CDS             24604..25083
                     /locus_tag="PLV81_06365"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOM09762.1"
                     /translation="MNWKEFLFTGLYTGYFPKGSGTVGALLALLLYVILHQICGRYGV
                     LANVVLVALVTYPAIKLGDDAEIYFAQKDPQEVVLDEMLGFWVTMLFHPFSWSTAVMG
                     FFFFRLFDIIKPYPIRKLEHFRGGLGIMIDDIIAGVYANITIAIIMVIMKAYGIVLV"
     gene            25145..26011
                     /locus_tag="PLV81_06370"
     CDS             25145..26011
                     /locus_tag="PLV81_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HOM09763.1"
                     /translation="MKRTYTIIGVFAIILLTFYIAYAQKTMGDSSHKKNYCIECHIKL
                     NGKPRAVVLEWENSIHAKNNLECHICHGGNPDADDAKAAKDKKANFVGKPKKKDIPEF
                     CGRESCHDQALAQLKKGPHYNSVLKIGSPNCVDCHGDHNIQQSTYHIITDKTCSSCHS
                     VEYSRELVNSIISIEKNIEDIEKSLDYMNERNVESKGMTQRYNELKSYFHQLVHVFSK
                     QEIDFTKKLVEVEIEYLDKELQSKIASIKRLDLIYILTSAFSIGVIITFTVYIALIRR
                     RRAKIQKDFQSR"
     gene            26035..26550
                     /locus_tag="PLV81_06375"
     CDS             26035..26550
                     /locus_tag="PLV81_06375"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOM09764.1"
                     /translation="MADSYFDRFKRWYLFNTEKIKYVGKKNDKHACILCAIRDHAEDV
                     VSLEIHRTDTFIVSVNLYPFNPGHCMIFPLQHIEHLHDLSLDDVLALHRLTIKTIDIL
                     KEEFKPSGFNVGYNLGSWSGASIPHIHLHVVPRFPNELGFLDVISHTRVMVVDPHDVC
                     ERLKKRFNNNQ"
     gene            complement(26631..26825)
                     /locus_tag="PLV81_06380"
     CDS             complement(26631..26825)
                     /locus_tag="PLV81_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09765.1"
                     /translation="MLPHPMRAGDFTCLLCGSKLNLKISELSIGANSGTCPMCGEPFT
                     IKLNKKDIELLLEAEETSKQ"
     gene            26966..27685
                     /locus_tag="PLV81_06385"
     CDS             26966..27685
                     /locus_tag="PLV81_06385"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM09766.1"
                     /translation="MTKLRKITLTLLVISSIIALIIIFDGDILKKIFVLKSVRVVTQS
                     EIKRMEEELSELTSKSATNPEVVNKIGILYQNLGGKYNEKGAWDPAIDALQKALGYGR
                     NNPLVHYQLAIAYANRGTQLENKDDIDKAQIHYEKALQMDPNFIEAKYGLAILLFYEK
                     DNRSKAVQLMEEITKQKPTFYRARFALGRFYYENKDLNKSLSVYQDLYTDLEKMDSPL
                     AKEYKQACKENIQRITTELAR"
     gene            27691..28779
                     /gene="dprA"
                     /locus_tag="PLV81_06390"
     CDS             27691..28779
                     /gene="dprA"
                     /locus_tag="PLV81_06390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOM09767.1"
                     /translation="MNNKKYAVALSLMSSGHWHFYSKSDPETIYNEYVLPEERKVQEY
                     YTSWYSMSPLEEAEKIIELCNKMNVNMISYWDEDYPALLKEISYPPAVLYVRGNLPNK
                     LSLAIVGTRNDDTQSGSIAEKLAGILAQYDIAIVSGLAIGIDRRAHIGALKAGGSTIG
                     VMANGIDMLYPASNRDIYTDIINSGNGCVLSEYPPKVRITRWTFVRRNRIISGLSKGV
                     IVIKAGEKSGALITAQYALEHNRELFVCTGHSFDEGYKGCYKLLKEGAHPVFNEQDIL
                     EILSVDKLYRQPTLFEGMEFNKVTSNTHFAAYEDDDIVKTLKQGSMDIDSLIRAVGKN
                     SGEVLEKLTTMELEGVIIRNGNLISLNK"
     gene            28788..29399
                     /locus_tag="PLV81_06395"
     CDS             28788..29399
                     /locus_tag="PLV81_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09768.1"
                     /translation="MIIKRVLIIMLALLWVISFIQIFSVPPYIGDIFEIYKSGYFKEL
                     ERNMYIITDSFLHIKTMTKPEYAWIYLDDLQKRSGISIAVYDAQGNLIKAPGMSEIVN
                     NTEIMNVCNDIKPQAQFTVSGRLYQGIIPVYRKSECNFCHQPSQKPLLGVITYAIPFD
                     GYIYYTSERIILFTIITFAISIMLFVVIRWNPYKDIKELFDKK"
     gene            29462..31564
                     /gene="topA"
                     /locus_tag="PLV81_06400"
     CDS             29462..31564
                     /gene="topA"
                     /locus_tag="PLV81_06400"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOM09769.1"
                     /translation="MADHKKTLVIVESPTKAKTIHKYLGKDYIIASSMGHIIDLPKSR
                     LAVDVNNDFKPEYIPIRGKAKLINDLKKKASEANNVLLATDPDREGEAISWHLSNVLK
                     EKNPDIKRIEFHEITEDAIKEAVEKARDINMDLVNAQQARRILDRLVGYYLSPILWKK
                     VKKGLSAGRVQSVALRVICEREKEIENFIPQEYWTLDALFEHHKQEFVASLVSENKNK
                     IELKTKNDVDRVLKLIEGKPFTVSNVEQKERRRKPQPPYITSKLQQDAARRLGFSASK
                     TMMVAQQLYEGVTISGEGQVGLITYMRTDSTRVAPAALASVREYLGKNFSKEYIPESP
                     NFYPNKKGAQDAHEAIRPTNVFRTPESIKNDLTPDQYKLYSLIWKRFVSSQMTSEVSL
                     VVTVTMNVEHFEFRATGSRVLFNGFTVIEKEESAEKKLLPPLDVGQVLIPKSFDPQQH
                     FTSPPPRYNEASLVKFLEESGIGRPSTYAPTINTLLKRYYVTKRGKQLVPTVLGRLVN
                     DIIVSYFESLINIDFTAKMEEKLDLIEEAHNDWVSMIKEFYEPFKLTVHHAEEHIEDM
                     KNILDEETDLVCEKCGSKMVKRLGKYGFFLACSAFPKCSNAKPLPLGKCPKCGGDVIK
                     RTSKRRGSFYACNRYPECDFATREQASEHACPKCGSLLFIRKIKKKGEMLVCLNEQCG
                     YSVTLLDEDKTDAEAHVS"
     gene            31570..32478
                     /locus_tag="PLV81_06405"
     CDS             31570..32478
                     /locus_tag="PLV81_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOM09770.1"
                     /translation="MDHYIDKFMQYLIAEKNASPLTVESYGKDLNQFIEFLYEAYDPE
                     SVTIAALTTNDIRDFIDYCFEKGNELSTIERKVATLKSFFKYLEFHNYIDNNPARDIH
                     YSRQKKFLPTFLTFDQIQALVAFECKNFSDYRDKALLEVFYSTGARVSEIANANIDDC
                     DFISGRLKVLGKGSKERIVFMNETSVTSLQEYLNERKKKFNCLTSPLFVNNNGKRLTT
                     RGIFYIIDKRANMAQLWKSVTPHVLRHSFATELLNRGADIRAVQDMLGHESISTTQKY
                     THTTTERLKALYQKYHPHAHRRHDNE"
     gene            32471..33016
                     /gene="hslV"
                     /locus_tag="PLV81_06410"
     CDS             32471..33016
                     /gene="hslV"
                     /locus_tag="PLV81_06410"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOM09771.1"
                     /translation="MNNTSMRGTTVLAIKKDNTIAMGCDGQVTLGDTVIKSKAVKLRK
                     VYDDKILTGFAGSVADALTLFERFEQKLESYSGNIPRSVVELARDWRLDRALRRLEAV
                     LIVGNPQHLYLISGNGEIIEPDDGIIGIGSGGSYALAAARALVRNTTLNAYDIVKQSL
                     LIASEICIYSNSNIIIEEIKA"
     gene            33016..34428
                     /gene="hslU"
                     /locus_tag="PLV81_06415"
     CDS             33016..34428
                     /gene="hslU"
                     /locus_tag="PLV81_06415"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOM09772.1"
                     /translation="MSDNTIKFSEDGDLTPRQIVSELDKYIIGQKDAKKCVAIALRNR
                     SRRKKLDPELQEEIAPKNIIMIGPTGVGKTEIARRLSRLVNAPFIKVEITKYTEVGYV
                     GRDVESMIRDLTSIAVNMVKKEYGAMVEEKALKNAYDKIVDIIIPKKGFEFDERQISG
                     FHVERESIFESDTLYEQMRAQYLEDLKSGKLDDLEIEYESESPNVIPVMSIMGGSGLE
                     DIDLQIQSMLGDLMPKKSKRKKSTVKDALRIFIAEETEKLIDMEKVTNEAIRRVEDMG
                     IVFIDEIDKIAGAESKTGPDVSRQGVQRDLLPIVEGTTVNTKYGPVKTNHILFIAAGA
                     FNISKPSDLIPELQGRFPIRVELSSLTEEDFRKILTVPKNSLTLQYKELLKTEGVTID
                     FTDDGIAEIAKTAQILNEEHENIGARRLHTIMEKLLEDILFDAPDLDDAHKNFVINAE
                     FVKAKLGPTIKNKDLSRYIL"
BASE COUNT        11402 a         5572 c         7095 g        10814 t
ORIGIN      
        1 gttgctattg ccattgatgg gtatcaggat ttaccacggc ttcctgatgg aagctatgat
       61 cttgatccaa ccaaaaagcc aatacaagtt ccattgactg aagatgaact gcggcaggcg
      121 gaaaacattg taaaaaaagc catcaatttc aatcccgcgc gtggagatca ggttgctgtt
      181 gaaaacatca tgtttgatag gaccaagcag tgggaacagt tacgtgaaga attcagaaaa
      241 aaagaacaat ttaagcgaat gctgcttgca gcactggttg ggattttggc attattcttg
      301 ggtgttgttt tattcagggc tatctctcgt gagcttgccc gtagacgtag aatacgtgaa
      361 gaacaactgg cactggaaca gcagcgtatg cgtgaagctg ccttacgggc tgcagaagaa
      421 gaaggtattg atgtagaact atcccttgaa gaaaaagcac gattagaatt gcagcaaaat
      481 gctataaatt tggcacgtga acgcccggat gatgtagcac agttgctcag aacatggtta
      541 gcagaagaat aatgttgtaa ttggtacatc ctgaaagacc gatatactaa taagctgata
      601 ttgactttaa aaaggagatg gtatatacta taagtaggta gaatatataa gaatggctga
      661 tttatatgta agtatatcgg ggtaatgtgg agcttaaaat atgttacaac aaaagaaatc
      721 gcaattaacg ggcaggcaaa aggctgctat atttttggta tcactaggat cggatgtatc
      781 gtcagagatt tttaaacacc tgagggaaga tgaaattgag cagctgacgt ttgaaattgc
      841 ccgtttagat aaagttgaac ctgaagaccg ggacaaggtt ttaatggaat tccaggagct
      901 tatgatggct caggaattca ttgccaccgg tggcattgat tatgcacgtg aagtacttga
      961 aagggcgctg ggtactcaga aagctattga tatagtaaat cgtttaacgt catcgttgca
     1021 ggttagaccc tttgatttta tccgacgtac agaccctagt catcttttaa acttcattca
     1081 aggtgagcat ccacaaacaa ttgcattgat tttagcttat ttagatccac agaaagctgc
     1141 tacaatttta tcgggtttaa gccatcaaat ccaggctgat gtagcgcgcc gaatagctac
     1201 catggaccgc acttcgcctg atgttttacg tgaagttgag cgagttcttg aacgaaaact
     1261 ttcaacatta gccagtgaag attatacttc ggcaggtggt attgatgcaa ttgtagaagt
     1321 attgacccat gttgatcgtg gtactgaaaa aataattatt gaagcacttg aagaagaaga
     1381 tccggagctt gctgaagaaa taaagaaacg tatgtttgtt tttgaagata ttgtattact
     1441 tgatgataga gcaattcaga aagtgttgcg tgaagttgat acacaggatc ttgctaaagc
     1501 acttaaaggt gttgatgccg aagttcagga aaaaatattc aggaatatgt caaaacgtgc
     1561 atcagcactg ttgcgtgaag atatggattt tatggggcca atcagactga gagacgttga
     1621 agaagctcag cagaaaattg ttaatataat acgaaaactt gaagaagctg gcgatattgt
     1681 tgtagcacgt gccggcgagg aggaactcgt tgtctaagct tgtttttaaa cctactgaaa
     1741 ttcaggttat atcggcacca aaacagattg agttgcctga aaaataccag aaaaatgttt
     1801 taaccgatga ggaatatgct gaatttgaag tagatgaaga gggtaaccct gttgaggtgt
     1861 atcagggtcc atctatagaa gagatggaag ctgagttaga acggtaccgg cgtgaaacag
     1921 aagaagaagt gcgccggatg ctggaagatg ctaaagctaa ggcagcacag atagaagagg
     1981 aagggaaaaa ggcagcattt ctggaattac agcaggcacg tgaacagata aagattgaaa
     2041 tggagcgctt taaaatagaa tccgaaaggg agattgagcg cggtaaattt gaagcagaga
     2101 agatgatcaa agaagctgaa ttgaaggttt cggagataga acatgaagct tataaacgtg
     2161 gttatgatgc aggacgtgaa gaagggtaca aagaaggtca ggcggaggta atgcgcctta
     2221 ttgatagatt ggggaccatt gtttcaacag cagtagatat tcgtgatgat ataatcaggt
     2281 catctgaaaa gttgatgacc gaaatgatac tgatgattgc acgtaaggta ataaaggatg
     2341 agatagttga acgtcgtgaa gtagttatta acaatattaa agaagcatta cagcgtgtta
     2401 aagatagaga ccgtattgat atacgagtca attttgctga tttagatatg accacagccc
     2461 ataaagatga acttataaag atgatggagt cactgaagaa agttaatatt tatgaagatt
     2521 caagggttga gcggggtggt tgtattattg agacagatgt tggggctatt gatgctcgta
     2581 tttcaacaca gcttgaatca atagaagaag ctattcgtaa tgcacgtcca atatgatagc
     2641 ttaataacga ggtttgcatg atacagattc taccacatga aaaagttgat atattttcca
     2701 agtataagga tgtgcttgaa gaagttgata cgttgcgagc aacggggcgc gttgaaaaaa
     2761 ttactggttt aactattgaa tcgcatggtc ctgatgtgca gtatggtgaa ttatgtcgca
     2821 ttagattgaa gtctggaaat tacctgtatg ctgaggttgt gggttttaat aaaaataaaa
     2881 taattcttat gccaattggg gatatgaaag gtgttgtacc gggggcagat gtttatgcag
     2941 ttggttcgca gctttttgta ccggttggag aagctcttat tggaagggtc attgatggat
     3001 tgggcaatcc cattgatggt aaaagtaaac tttttttaaa agaaaggtat ccagttgaca
     3061 ataaaccatt aacgccgctt gaaagggaga taattgattc gccattgcct actgggatac
     3121 gggcaataga tgggcttata acagtaggta aaggacaacg aattggtata ttttcaggtt
     3181 ctggtgttgg aaaatctact ttaatttcta tgattgctcg aaatacggct gctgatatca
     3241 gtgttattgc gcttattggt gaacgtggaa gagaagcaaa agattttgta gaaaaagaat
     3301 tacagaatga aggtttaaag cgttcagttg ttattatttc tacgtctgac caaccaccaa
     3361 tggtgcgttt aagggcaggt tttctggcac attctgttgc tgaatatttc agagataaag
     3421 gattgcatgt taatttactc atggattcta ttacacgttt tgcaatggca cagcgtgaag
     3481 tgggacttgc agccggtcag cctgcagcaa cccgtggata tccaccttca gtattttcat
     3541 tattgccaca attgcttgaa cgtgcgggta ataaaaaagg tgcagggagt atcacaggct
     3601 tttattcaat acttgttgaa gcagatgata tgaatgaacc tatagctgat gctgttaggg
     3661 gtattctgga tggtcatgtg gtactggaca gaaagcttgc gcataaaaac cattatcctg
     3721 ctatagatgt acttggttca atttcccgtt gcatgaatga tgtgatagat gaggagcata
     3781 aaaaagctgc caacacattc aggaaacttt tagctgctta ccgtgaagca gaagacctta
     3841 taatgctggg tgcttatgca cgaggttcag accctgatgt tgataaagca attgatatga
     3901 aaccagcaat ggatgcattt ttacagcagg gcatttatga aaaagataca tatgaaaata
     3961 ttaaaaataa attatccgaa ttgatgcttg gaacaaaagc caagccttca aaaaggcagg
     4021 agtttgttga tagaacaccg tattttgcac aaagtcgtgg gagatagttc ctgaggatag
     4081 aaaatgaaga agtttgaatt cagattacag accttacttg atttacggat ttcaaaagag
     4141 catgccatta aagctgaatt agcacgattg attgcaaagc agaatgaaga aatagctttg
     4201 cagcagaatt ataaacgaag agttacagaa tactatgcca ggctttcagc taatatgcgg
     4261 cagggcacat tttcatattc agaagcgctt atgtgtgaac ggtttgccta taatgcagaa
     4321 aaagctattg cattatcaca aaacagagtt gaacaaatgc agcctgaaat tgaaaaggtg
     4381 cgtcagcgtt taatacaggc atcaaaagaa aggaaagtgg tggaacgttt aaaagaaaag
     4441 aaatatgaag aatatatgta tcaatataac agagaaatgg gaaaggaatt agacgatctt
     4501 aatcagagaa tgtataataa cgcgtgaggt aatgctaatg gatataaaca gcatcaaaca
     4561 ggttcaggat agaatcaaag aaattaaaaa tagatttaaa accattcaga atacctatgc
     4621 aaaagtgtcc ccatctacaa gtatggttat ggccgatagc aaagtggctg aagccagcaa
     4681 agtgactgct actgtacagg aaaatagtga accgcagaaa aataatacta ctgtaaaaat
     4741 gacagaagaa atcctgaaga atgtgcttaa ttcaaagaag gaaagtacta aggaaacact
     4801 tgacgttcag cagattcaaa acttgttgca aaaagctatc acccagtata ccagtaatgg
     4861 caaaatattt aatatagagg aatagtggtg aatgaagtca accataagag gttaatacca
     4921 tgaaactagt atcagattca acaaaagtaa tatatttggc aataatgatt gtatttatca
     4981 ttattgttgg attattctgg ttggattata ttggcttaat taatattggt aaaatgtatc
     5041 atcaatatat tgcaaaggaa gctccttctg ttgtggatgc tgctgatgat gaaccatcac
     5101 tgattgagcg ggaagaattt aaaaaggaaa aagataagct attgcaaaga atagaagatt
     5161 tagataagcg tgaagcaaag atagtagaag ccgaaaagat aattgcaaag gaaaaagaaa
     5221 aacttgaaca attacagaaa ggacttgatc aggaaaagaa ggcactggag cttgaaaaga
     5281 aaaaatattc aggctataaa aagaatgtta tagaccttgc caaaaaaatt gaaagcattc
     5341 caccgcagga tgctgttgat ataatggttc aatgggaaga aaccctgata atagatgtgt
     5401 tacgtcagat tgaccagaat gctgcaaagg aaggcaggat gagtatatca acctatttgt
     5461 tgtctttgat gccaaaagaa aaagcaagcc gggttatgta tttgatgact caattgtgat
     5521 tcttatttac aatattcctg tatcattgcc aaaattatat ttttacccaa tccagtttta
     5581 gcagatgttg gaactacgta ttctttactg atgttgaggc tggtgcaaat tcttgatata
     5641 ccattattta tttcattttt tgaaacttta tcacatttag tggcaagaat cattacattg
     5701 atattattgt ttaaagcttt tgtaattatc gcttgctcat tttcatctgg ttcgcgcctg
     5761 atgtctataa ggagaaacag aagcttaagg ttaggccgtg aaataatata tgaatggatc
     5821 atttctatga attccagccg cttattatgt gaaacttttg cataaccaaa tccagggcag
     5881 tcagcaaagc tcatcttttc attaatacaa aaaaaattaa ttgtttgtgt cacgcctggt
     5941 ttactcccag ttttggcaat attttttctt cccagaagag tgttaataag ggaagacttg
     6001 ccaacattgg atcgaccggt gaatgcaaat tccggtaaag tatattttgg atactgtgat
     6061 ggaatatggg cactggtaac aaaacgaata gtattaatct tcatgattta tgttcttttc
     6121 catcagaatt gcattatcaa aacggtaata attctttctg aacccaactg agtgaaatcc
     6181 taacgatgta taaaatctta tagcactggt attggtatca gcaacttcaa gcacaatttt
     6241 tttttgatta tctgtatggt taatgacatg ttggataagc tgtgttccaa ctttatatcg
     6301 ctgaaaatgt ggtaaaactg caatgcgcat caattgaact tcatctgcaa tataccatgc
     6361 aagggcatat ccagcaaatt tattatcaat ctcagcaata aataatcttg aaaaatgtaa
     6421 atctaattca cgggtaaagc tttcagttga ccatgcattt tcgcgaaaaa cataggcatc
     6481 cagtgctgca atgttttcaa tgtcttcgta aagagctttg cgaattaaaa tttttttatt
     6541 gggcatcctt ctacttatct ttgaattttg gttcacgctt ttcaagaaag gcagaaattc
     6601 cttccttttt gtcttcagtt tcgcataact gcccaaaaag ttcacgttcc aatgtaagag
     6661 cagaataaaa ctccatggtt aaacctttag ctattgcctg catagcagct tttattgcca
     6721 gtctgcttct tgcggctatg gcttttgcca gttcttttgc ttcgtcaaca actttgcctt
     6781 tttcagttgc acggttaaca attctataca attcggcctg ttttccgctt ataaaattac
     6841 cggataaaat aagttcgtaa gcagcacctt tgccaaatag acgtgtactt cgttgagttc
     6901 cgccatatcc cgggataatg cctaacttaa tttcgggcaa acccagctgt gccgattcgt
     6961 cagctaaccg tatatggcag gcaagtgcca gttccagccc accacccagg cagtagccat
     7021 taatagctgc aataaatggt ttgtcagcat tttcaatgat gttcagcaca tcctggccgt
     7081 ttttaagaaa gtttgcgcca tcttctctgg tattctgttc tttaatttca ttaatgtccg
     7141 caccagcaac aaacgctttg cctgcaccgg taacaataac aactctaact gaatcgtttt
     7201 caatgagctg ttcaattgtt tccctcatat cttttaaaac aacatggttc aaggcattca
     7261 ttggtgggtt atcaatagtt aatagagcta ttccattttc aacagtataa tgacattttt
     7321 tttgtgtaga gtcacccata tcacacctct tgaattaaat tttctaaaat tttcaaatat
     7381 ggtgcaggtt tgtcaagatt taattgtaaa tataaaattt aaattaattg tgactcttta
     7441 tatttaataa gtttgtatac tgttgtatta tagggagtat ctatgctata ttgttgagca
     7501 aatgtaataa tagcaccatt aatagaatca atttcagttt ttcgcttagc aagtatatcc
     7561 tgtagcattg aacaaaaatt attctgcgtg tttctgcata ccgttgtagt tgtttcaatc
     7621 atatggttac tatctgtcat aataccgtta gcctgtgcca ccaggcatgc ttcctgtatg
     7681 atacctgcca taatctttac tgtattattg ttttctataa gtttgccatt aggcacttcc
     7741 agcaatgctc ccagtgggtt aatcgctgca ttgataatag ctttttccca taaggctttt
     7801 ttggggtcag gggcgatagt tactgtcagg ttggcatttt gtagtaatga atgcacggtg
     7861 gaacaatggc tataactatc gccaccaatg atgatagagc ctgttcctcc aaaacgtaca
     7921 ctggctggtg aaagttttgt ggcgccaatt gtggtggtcc cgtaaactaa cgggttgtta
     7981 atcatagacg aaatcaactc gacattgcca ataccgtttt gcaatgttac tacgattgca
     8041 tcgggcttaa tataatgttg aatagtatca attgcatctc tggtggcaaa acttttaatg
     8101 aacaaaaaga tgatatctgc tgaagcaaga attgcaggat cattgcttat agtgggataa
     8161 aaggtttgtt tttctgaatg tatttcaaca gacaaaccct gctttattgc attaactgta
     8221 ttgggattag tatcatacaa tagtatggta tacttgccca ggaaaaatgc aaacagggat
     8281 cccattgcac ctgcgccaac tattccgata atgggtttat tattcatgat ttttttttac
     8341 atcatagtta ctttatggtg tcaatactgt taatatattt gctaaatagt attatatata
     8401 aattcatgaa tattttctta aaaatatttt gactcctgca accatatagt atatttgtat
     8461 taaaattttg ataatgtgat aaaaatgtac tatttttatg atataatata actattaaaa
     8521 tttgattagt tatcattagg attgtaaaaa aattgctaac gagaaattat taccatggat
     8581 aatcctgtca gtttggaata tactacgctc cttaatacca tacaggcacc agtttttgtg
     8641 gtagatgctt caaagaacat tattgatata aataattatg ctaagcgttt atttcattct
     8701 cttgatgtaa ttgggaaatc gtgtaaggat atcttcccgg agtgccaaaa atcatgtacc
     8761 tgtcctgttg atgaagctga ttacaaaatt ccaactgata gccagcgttg taagcaggta
     8821 atggtactga atacggtaaa agaggaggtg gaattgtgta ttattccggt acaaactgca
     8881 catgatattg tatttcttca tattttaact gacaatgata tacagaacag gcagaacctg
     8941 gtaatgcttg aaaaaaacat gactatttca acgctggcat cgggtattgc ccatgaattt
     9001 aataatatga acgcaggcat tcagggaatt gttgaattat tgctttccca ggaagagtta
     9061 tcttcggctg gtgtaaaaga tcttaaaaca atattgaaga tagttaaacg tgccacacat
     9121 cttattgatc agttatcaat gttagctcac cgaaaacctt caaaacagat gctggttaat
     9181 attgaagata tagtcgatga ttgtataagt attataaaac cacagtttat ggatgctgga
     9241 attactattg atgttattca taaagacaga ctaccagaat tatttttaga tgccaataaa
     9301 atcaccctgg ctcttatgaa tattttttta aatgctcgtg atgctatgat tgacacagaa
     9361 caaaaaaaat tagcaattat taccggtcag gatgatacat atgttactat aactattaaa
     9421 gattcaggtt gtggaataaa agaagaacat atacagagga tatttgagcc attttttacc
     9481 acaaaaggcc cattggggca ttccaatata ccaggtacag gactggggct ttcggttgca
     9541 ttgggtatta ttgaagaaca tggtggaacc ttagcagtag aaagcacagt tggcgaaggt
     9601 actacattca ccatacaatt gcccgttaat actgatgata aagttgcttc tgaagttgcc
     9661 ataaattata ctgaatttga tttttcgggt aaaaaaattt tgattgttga agatgatcct
     9721 gaatttgcta ctattttgca aagggcgctg attgctaaaa atgctgaagt agtatacgca
     9781 ggaaacggtg ctgaaggatt atattatctg gataatcaaa ctgtagatct tgcattactt
     9841 gatgttcacc tacctgatat gacagtatgg gatatattga agaaagttca gtatatgaat
     9901 aacagaccaa atataattat tgtatcagga aattatttgg caatgaataa caactatatt
     9961 ggtatagttg attatgtgct gctcaaacct tttgatttag aagaactgtt tactgcaatt
    10021 aaaagcagtt taactatata gtatacatat aaaaatagta ttgacaaaaa tataaatctg
    10081 tataatatct tcacaaattt tggcagagat atcaggagca tttatgacaa aaaaagatac
    10141 tgttttgctt atagtaaatc ccattgctgg caggggaaag gcactcagat ccgtagaaag
    10201 gattgagaac gattttaaaa ctttgaacat caaacatgat gttgttttaa cggaaagagt
    10261 atggcatgca gcggaattag cagaagatgc tgctaaaaaa ggataccgtg ctgttgtggt
    10321 ggccagcggt gatggtactg ccaatgaagt gttaaatgga ttgatgttag ctcggaaaaa
    10381 gggctataat aaaactgcca tgggtcaaat cccggtaggt acgggaaatg attttgccta
    10441 tggcatggga ctacctaatg atatagatga gtgtataaaa ataatagcag acttcaatgt
    10501 ccgaaggatt gatgttggtt ttattaaggg tggagattat cctgaaggca gatattttgg
    10561 aaatggcatt gggataggtt ttgatgctac tactgggttt gttgcaagca gaatacgctt
    10621 tttaagcggt atgatggtat atctgcttgc tgctttgcag acaatattta tatattataa
    10681 agcaccacga atacgcatga tctataatag caaagaaacc attatggatg ccttgcaggt
    10741 ttccataatg aatggtaaac gtatgggtgg tggttttcat tttgctccag atggtagccc
    10801 ttttgatggt ttgcttgatt tatgcatggt ccgttctatg aatcaattta agatttttgg
    10861 catgatacct tattttatac gtggtacaca ggcaaggcgt aaagaagtaa tgcaggacag
    10921 aacaacaaag attgaagttg ttgctgaaaa aggtagcttg cctgtacact gtgatggcga
    10981 aacgatctgt tattctggta aagaattgtt tattcagctt ttcccggcag cattggattt
    11041 tataacaaag gcttgatgca taagaaggta gatgttcaat gctattttta taaataataa
    11101 cactgtaagc attgccagtt cacataacaa ttaagatact ataaataatt gtgaatgtta
    11161 cttctataca taaactgatt ttagtttttc tatctattat tattttaatt agtatttatt
    11221 ctataagcta tgctcaaact gcgtatcttg aaactaatac tggtgcaggt ttttcacagg
    11281 gaacaatagg tgaatatgtt tatgataaca atcgcatgct aagcagatta aactggaata
    11341 ttgattattt atggtattat cacttttcag ttgctctatg gtataactcg ttttttgcag
    11401 gaattcaggt gcacaatggc atcaatgata aaatagggac aattgatgat tcggactgga
    11461 cagatgataa taatccttca ttaaaaacac attattcaaa gcatgataat tatcttgaaa
    11521 gatatgcttc ctatgattgt aacattggtt ttaaaagtga tattactgat acaataaacg
    11581 taaaaccata tatagggttt ttatataaat ctattaagat gtctgcaaga gatggctatc
    11641 tggagtatcc tccaggttca ccagaggatc cagtctatgg agttggaatt atatatgaac
    11701 agagatatta tatcccttac atagcattgg catcagaaat tattgtgaca gaaaccatag
    11761 gaattatggc gggaatatct gttagtttat ttgctgttgc tgaagacata gataatcatg
    11821 ttcatcgcaa tccaggcgat gatattgatt tttatgccaa ctatgatggg attatttatt
    11881 tacatgctta tggaggatgt ttcttctctc tgagtgatac aataaaggtt gcgcttgatt
    11941 tactatatga atatgtacct gagcagaaag gtgatacgta ttatatcaat acgtcaacag
    12001 ggataaaatc acctgttttc aataattcag caggattgaa atattcagtt ttttcagtac
    12061 agctttcggt agtatatacg ttgaaattat tataatgaaa gggtaagatt cttaggttgt
    12121 ccccataaag tcggggtccc tttagaatga cagggtgtat tgtcacatga tgacttcatc
    12181 aggatcagct accggatttt atcaactgtt cgttgagtga ttgcgcaagt aattgtattg
    12241 agacgcaatt gtcgagtggt tatgcagcaa ttgtatcgat attcgatcgt cgaatgtacc
    12301 aaagaagcga gaaatgtatc aacgaggaat gtatggagac gaggtggtcg agtattgcga
    12361 acaaagcgaa gactgtatcg agacaactca gcttcgactt cgcaatgaca ggagttaaat
    12421 aaaggaggtt aactactatt atggacagaa tccaatatag aaaacttaac aaatataagt
    12481 atcaattaat gcgtgattac gtttataaaa caacaataac gttagatcat gatgctggag
    12541 taccagattt tgtaacctta cgaaaagatg gacaattaga gatatataaa ggatatgcct
    12601 gggatggccc cagtggtcct actattgata ccaaagattt tatgcgggga agcttagttc
    12661 atgatgcgct gtatcaactt atacgtgaag aaaagataga aagaaaatat aaagaatatg
    12721 ccgacagact tttgagtgat atttgcagag aagatggcat gagcaagttc agggcatggt
    12781 atgtgtactg ggcagtgagg ttgtttggca aagcaagaaa aaatcctgat gacaccagta
    12841 ataaacttga agaagcaccg taattttcat tctgtgtatt tgattacttt acctttaatg
    12901 tgaataatat tcactgtacc aacaatccag tactggcgtt caatatacca ggatacatca
    12961 atcaggtcat cgccattttt ttgattaatg gcatctgaaa tagctctatc aaaactggcc
    13021 agtggcgtaa cattgaaaaa ccataaaaga ttaaagttac tggtaaatga ttcagcttca
    13081 ccaactatct ggtatgaaat atcttttaag gggcgaacat gtggttcata tccagctaca
    13141 tgatgggtgg ttacacatcc taaaaaaaca atactgcata gtatgacaat aaacagtaac
    13201 tgtcgcatag ctacctcttt tgctttactt gttcttttgt ttcaacaggt gctgtcttta
    13261 ttacctggcc ggtaacgata acttttgtga tgctaaaaag aacaaagtaa taatgctttt
    13321 cataccagcg tacatttatc aatgcatcac cattttttga agcaatagct tcatgtattg
    13381 ctctatccat gtcaggcctg cccactgacc acaggccaaa aagagaccat gaccagtcac
    13441 taccttcagc ctggcctaaa ttttcaacga ttatacggtt gtgaagtggt gttgatgatg
    13501 ctgtcatgcc cattggtgtt acacaaccag ttaaaaagac aatagttatc ataattatta
    13561 gtggtacaat gtttttcata gattaactcc tttcaaatta gtaacctcgt tgatagcagt
    13621 ggtgtagttg ataacaaaca attttaaaat ttaacctcaa caccaaaatt ggggattaaa
    13681 tttaatccct cctgagtttt taattttgga ttatcttcac catgtggttt ataataattc
    13741 catgctaaat catttttagg cttatagttg taaatattaa tgacttcaat ataccatgaa
    13801 acataacccc actcatggtt ggttttatgg ctgtagcgta catccagacg atgttcccac
    13861 ggaaatcgtt ttgaatgaag gtggtcataa tcatagatag gaacatatcg ccctgagtta
    13921 tcagggcttt catcagagtc aataactggt gtatacggga atccactgta agcttgaaag
    13981 cgtgcactga tggtatgttt ccccactgtg tagccaataa ccatttttaa ggaatgtggc
    14041 tgctcgtatg catatgggaa ccatatatct ttgttgggat ctattgatgc accggtttta
    14101 aactttgatt ttgtgtatgt gtaatcaacc caaccaaaga aaccgtcagc attttcttcc
    14161 tggtctatgc gtattaataa ttcaaaacct ttagcctgta gttgtccgct acttttacct
    14221 tcctgtataa ggttatttga actatcataa acagggtcat cctggtacag gtccttgaaa
    14281 tgattataaa aaccttctgc ctttattgtt atagcgatag tttctgttca atactgattg
    14341 cattatgata tgcactttga ggcttgtatt catcacccgc acctgctaaa ttgggctgaa
    14401 agttaaatag attggaattt gtctggataa aacctgaata tttccctgtt gcatacgata
    14461 cagtggtttg tgaaggaaat tcaaagctta ccataagacg aggatccact gtttgctgtt
    14521 ttgtacgtgt cagatagtca gtacgtattc cgggtagaag tgtgagtccc ccaaatataa
    14581 atttttgctc tgcataagct cccagtacat ggttgttgaa tttttgacct aaagggaccc
    14641 tggtggctaa atcgggatca ccaaagtcag gaatattata cagtggtgca ttaagaacca
    14701 gtgtatatcc atcatttgta aaatgataca atgtgtattc taatgcaccg cgaaactggg
    14761 catgatcttt ccaccattcc aacttaattt tttcttttgc accataaata tatggtttag
    14821 aggttatatg caggtctttt acccagtcgg cagcatcaac agatgggata ttaaggtaat
    14881 tataactgtt tgtgagagat ccaaacataa caattgtgtt ttcaaagcgg cttacaggct
    14941 gccaccggta atatacacca agcgcatgtg atcccaaatt atttttaaat tcaaatgatg
    15001 caagcagtgg gtccacttct tctggagggg ttgattcatt aatgaatttt atataatcgt
    15061 atgctccaaa aaacagaaag gtaatggatt gagtttttgt aaaataatat ttccctttga
    15121 attgataatc ccagtattca ggtactgatt caatttcatc gccggtaagc aatttgtata
    15181 ttgcaggtac aaataatgat agatatgcat aacgtccaga tactatccag tagcctctgg
    15241 tttcttcctg gttagttgct tcattgtatt ctgttactgg tgatttgagt agtaggtttg
    15301 atgatattaa tccaacatca gcagaaccgc caaactcttt gactgtgtca atagtattaa
    15361 ttgatatgac agcaccaatg gcattggcat acggagatgg aaaacttgaa gaatacaggt
    15421 caatttcact catcaggtca ttattgatga tagaatgaat tgctccaaaa tgttgtggat
    15481 tataaacggg tatatcatca atgaaataac cattaaagct gggatcagca ccacggataa
    15541 caagaggtcc aaaaaaaccg ctggtgcgta taacgcctgg caatgatgtt aatgcattta
    15601 aagaatcgcc aaatgatgca ggtactgctt ttaaatcctg tgcggttaat gtataacgcg
    15661 atactttttg tatatcacgc tcgccagtaa tagttaaggc agcacctttg attgctattg
    15721 gttgtagcac aatatctttt tgcattatgc cattaattgc aacagtaacc gtcacgggtt
    15781 taaaccccaa tgatgagatt ataagcgtat atgtgcctgg tttttcaaac agtatgctgt
    15841 atgagccatc tgcttttgta taaaacttct gtttaatttc aggtatggct atagttgcaa
    15901 aatcaaccgg ctttttagtg gcctgtgaat agattgttcc ctgtatggca tattgatttt
    15961 gtcccagggc aatggtgctt acaatagtaa ctatcgccca tattagtatt aatttcttac
    16021 ttatactcat accttattcc agtttaaatt gcattggtaa ttccattttt attgctacac
    16081 gctgaccgtt aataacgggt ggtgtaaatc ttgcctgggt aagtattgaa tatgctgctt
    16141 ttgaaaattc tttgctcagt acagtgtatt gctctggtgg aagatctttt gatagacgta
    16201 ttgatagtat ttgaacagtg gaaactttgc catctggagc aatgacaatt tcaacgttta
    16261 catttgcttc aacatttttt tcacgagctg actggggata aattttcttt aaagctccaa
    16321 taggcctggg aggaacaatg ttgggataaa atgaaatatc aaagacttct gattcatttg
    16381 aaagaccaac ggctctatcg gttgatgatg gagtagcttc tcctgttgtg gttgatattt
    16441 cttttgtagc tatacgtttt gattgtgcgg taacggtttc aatattgata aactccaggc
    16501 tgtcagctac ttcaggcatg tcggcaacat agattggtgc ggtgaagaaa agtgtgaatg
    16561 ctgcaatgca tgatgaaata ataaatgaaa ctatataggg atgttgctgt tggaattggg
    16621 atacatgctg cagtagtata tccaatgttt gacgaaaagg cattattgtt cttcccgtgg
    16681 ttgagatata aatacaacat tgagaaagtc gtattgccgc aatatattaa taacattagc
    16741 tacaactgaa tatggtaaat ctttatccgc tgttattgaa actatcctgt ccttttcatt
    16801 ctggcgtatt gctaactggt ctttgatatc gtcaatggat gaatctttac catcaaaata
    16861 tagagcgcct tcttttgata tagaaatata aatactgtcc tgttctgcac cctgggtaac
    16921 tgctgtggga agctcaacag taacaccttt tggtggctcg gttgaggttg ttgccataaa
    16981 aaatactaaa agcagcatgg ccatgtcagc agctgatgct cccagagaaa acgatcgtag
    17041 tttttttaag aaggggctct gtaccatatt taccgctcat tatattttaa tgatattctg
    17101 gataagccac tttcacgaat ggcactgagc gtatcaacaa gtctttcgta tttgatagta
    17161 ggtttcatat aaattattga tacagcttct tttgtttgcg atttgcgtac tgttaaatat
    17221 tgtaataatg cttttctgtc taaggccttg ccatcggcaa taaatccatt atcctgaggg
    17281 taaatttcaa taacctgcga tggctcaact ttaactgtcc cagcacttag cgaggggagg
    17341 ctcaaaaaaa ctccctggcg tataataaat gaagcggtga caataaaaaa tattaaaagg
    17401 ttaaacgata aatctgcaat tggcgttgtg tcaaactcaa tgagccgtga ttgtttttgc
    17461 tgtggtttgt ttttacgcga aaaccagtac attatacacc ttccagtgat gtagtacttt
    17521 caatctgtga gtatcgtggt aattgttttg ttttgtggat aataagttca gatgcttttg
    17581 catacacatt ttgtaatata tgaacataga aatggtaaaa tgctaatgtg gggacagcta
    17641 tggataatcc acctgcagtt gtaataagag caatctgtat accaacggct actacttttg
    17701 cattaacggt tgttgctgct gcaattgagg caaaagctgc aatcatcccc tggacggtac
    17761 caaaaaatcc aatgattggt gctaaattac ccacagcatt aagcagaacc agcccttttt
    17821 ccagctgatt ataataaacc tgcatatttt tttcaaggtc atctattgct tcctcacgga
    17881 acttgtatat gttctggtta agtaattcat tcaacttatt ttcaatatgt gctgtatccc
    17941 atattttaat tttgtaataa aataaagcac gttcaataat tatggttaaa cccacaatac
    18001 ctaaaaacag tataggccac attaaaatgc caccctgctt aaaggtagcc cataaattgg
    18061 atgaattggg cgatagttcc tgggcaaata atggttgact tagaaaaaga ataatgaacg
    18121 ttactatttt attcatagat gacctcattt aaaaaaattc ttgaagctgg atggatgtta
    18181 ttgaacatat tccaaattta cagttatgtc aatcacttaa ttgagtaaaa cgttttcaat
    18241 taattttcag aggtaacatt acatgaacat tcttataact ggtactgcag ggtttattgg
    18301 tttttatact gcattgcgat ttttacagga agggcattgt gttattggta ttgataacat
    18361 gaacagttat tatgatgtat cgcttaaaga agcgcgtaat aacattttga agcaacatga
    18421 taattatatc ttttatcttg tagatatagc cagttaccag gaactatcga acatatttaa
    18481 aaaacattct tttgactatg tggtgcattt agctgcgcag gcaggtgtgc gctatagcct
    18541 ggaccatccg tttgactatg ttcacagcaa tattgttgga tttatgaata tacttgaatg
    18601 ttgcaggcat aatcctgtta aacatttagt ctatgcttct tcgagctcag tgtatggctt
    18661 aaattcctgt gtgccatttt ctgtgcatga caatgttgac catcctgttt cactgtatgc
    18721 tgcaactaaa aagtcaaatg aactttttgc tcattgctac tcacatttat attcaattcc
    18781 ttcaagtggg ttacgttttt ttactgtata tggtccatgg ggcaggcctg atatggctta
    18841 tttcaaattt acaaaagcaa ttttagagaa taaaactatt gatgtgtaca actttggaga
    18901 tatggagcgc gattttacct atattgatga tatagtggaa ggtgtatacc gaataacctt
    18961 taaagtgcca caaggtcaac ctgaatttga taccatgcat cctgatccag cttcaagtag
    19021 cgcacctttc aggatttaca atattggcaa taataaacca gtgcagttaa tgtattttat
    19081 tgaggttctt gaaaaggaaa caggcaaaaa agctaacata aactttttgc ccatgcagcc
    19141 aggtgatgtg cttgctacat ttgcggatat tgatgaactt actgatgcgg tgggttatag
    19201 accagtgact tcaatagaag aaggtttgca taaatttgtt caatggtaca aagagtatta
    19261 caaagtttaa ttattactaa gaatgtttgt gacacttcaa ttgcaggaag tgtattacca
    19321 tttaattgtc tttcaatgat tgcaggagtg tttcaatagt agttttatat gcaccctctg
    19381 tatcatattg catggatttc tgcaaatagg tttttgccat tactgtatca ttaatctgga
    19441 tgtaacagac agatgcattt atcaatgctg ctgccttgat atacgtgtac tgatccattg
    19501 tatatcgctc ggtggctaaa aaatacaata ctgctttttt atattcaccc aaattataat
    19561 aatttttccc aatgtaataa ttagcaatgc cctgaataac ctgattatca gtattctggt
    19621 ttatacattc ctgaaatgag tcaattgaca aatcatatag cttgtcttta taggcaatat
    19681 agctggcata aagcagattt ttatttgata ttgttatatt ttgaatcaat gtattccaga
    19741 attccctgtt ttcttctatt attgtagtgg taagttgtgc ccatgctgat tgtaataaaa
    19801 gaaataaaat tatataatat tgtaaaaatt ttttaatcat gatatatttc cctgatgcct
    19861 gccaatctta ttgggaaagt ttacaaaaga tatataactt ttgcaagtaa attatttttt
    19921 taaaacttca tgcaatagaa atacatatgc tttcttatat aaacatattg atactcatta
    19981 gatgttaaat actattgatc aaatttaaat ttctacaact tttgcattgg cagcattctt
    20041 tttaattgat tcaatccctc tcatacatcc gcttcttttg ctatagcctt caccctggcc
    20101 aattgtttcc ccatttgcag ctttaagtct gaaacggtat tcaccttttt tgtcatgata
    20161 aatttcaaac gacgaatctt ttttagaatt tttctttaat gattcaatac catcaagatt
    20221 aaaattaata tttttatacg tttatgaatg atgtgagttg agaaaaggaa tagacaaagt
    20281 agtaatgtat tttgatttgt ttgtatagtt cattaaacaa aacaataaaa atcattaacc
    20341 aagaatgttg ttaaatgaat aatatactca tgcaccaatg agtggttccc ttttgataag
    20401 tatatgtttg atattggcct tacccacaga gaaataaatg tgctgtgatt ctaaaagttt
    20461 aaaattgttc cactgtgata caattgtttc tggatgttgc tgctcgttag gttgttgctt
    20521 gggttgtttt attgtatcca tgttgtattt ccttactttc aggttaatgg aatagtatgt
    20581 ttatagcaca attttgtcta ctcaaaaaaa atataaaatt ttaagctaaa aatctttttg
    20641 tttttttgta ttgacttttt aaaaaagctt tttagtttga caaccgcata tatattgtaa
    20701 aaaatatgtc tatgcaatgt taaacggcaa acgttctttt aatgttgttt gcaacaatgt
    20761 atattcatca taggtttaaa taaaaaattt ataaagggca tcatgttgag cactcttaac
    20821 aaaaaattat tacgtatagc gatagctcca gtaattgtat tcctgtgtgt tataacagga
    20881 tattcctata aatttgactt tgtgacagtc aatgatattg tcaagcagat aaagaagact
    20941 ttctctgatg ttgaaagcta ccaggcaaat tttgtcatgg tttcggataa aatgggaaag
    21001 cggcagactc agagcggtac tatatggtat atgtcgccac attacctgag gatagatttt
    21061 gatgtgccat atggtcagaa aattgtttca gatggcaagc aggtgtggat ttatattcct
    21121 tcaataaatg tggttgctga gcaggattta aacagttcaa tgtttgcaat tggtactggt
    21181 tcaggtttaa acaggctttt ttcaaaatat cattataaat ttgatggcaa agaacaacca
    21241 cgtgtagaag gtaacaataa atattatgtg ttattcttaa agcaaaagga aacacgaagt
    21301 ggcttcagaa ctatgaagct gtgggttaac gaacagtatt ttataacaaa agctgctggt
    21361 gaaacagcaa atggcaaaaa cattacaatt aaaatcaaaa atataaatac taaagtagat
    21421 ttaaaaaaag gtttttttaa atttgatcct ccttcaaacg ccagaataat taaaaatcca
    21481 atgttggcag aggaataata tatggttgca ggaattggtg aaacattacg caatgcacgt
    21541 gaagctaaac gtttaacctt aaaagatgta tccaaagata caaatattgt tgttaagtat
    21601 ctggaagcac ttgaaaatga agattttgac aaattcccaa gcgaaacata ccttctgggt
    21661 tttttacgtt catatgctga ataccttcgt gttgatgctg atgagttgat tcaggcatat
    21721 aaaggatata agattggcga aagttcaaca ccacttgaag agttaaccag gcccactggt
    21781 ttatcaatag ttgctcagtt acggatgtat gcgcagcagt atcgtaatat tttatatttt
    21841 gcaggtattg gtattgctgt ccttattgta atatcgctgt ttataagcct tattacttcg
    21901 gatgtgcacg tgggaagcga tgattcaatt aaagcaatcc aggaagaata ttaccgtact
    21961 cagggcaaaa agattgccat taaaaatatt tggccgttac agctcaccaa tgatagtggc
    22021 atagtgttgt tatataatga agaagcatgc cagtttctgg tagataataa agagatatta
    22081 tttttattaa atgatattaa agataattca gtgatagtag agtttttgcc tgattcaaaa
    22141 aaatatgttc tggaaatgga aaagcctgtt atatgcagcc caatggaatc ctccaggaat
    22201 attgtgttga cgttaaaagg attaactgaa aacagggcaa aaatcagagt gcaattggct
    22261 gctgttgagc aggaaaaacc tgatgaagct gtcactgtga ctaccacagt tacaccacca
    22321 gctgaagtgg ttacgcatga tcagaaaaat ttaaaaatag tatttgaagc tgagtttgtg
    22381 cagaaaacat attttgaact gtatttagat ggcaatgagc gcgtcagggg aatgatgcca
    22441 gcaggtacaa aagaacggtg ggaagcatct gaatttattc agattaagat aggaaatgct
    22501 ggtggtgtta aggtaaaaat taatggcact gactatatgt ttggcctgcc aggtcaggtt
    22561 gccaataaag taattacctg gcgtaaagac ccacaggatc ccaataaata taagcttgaa
    22621 gtaaaagact ggtgatgtgt tttcacctga tgacatttca tactatatta tttcattagg
    22681 gtgccccaaa aatgaggtgg attccgagcg cttaaatgcg gatatgcata gcgctggttt
    22741 tgttgaggca catgagattg atactgcaga tataattttt atcaatacct gtggatttat
    22801 tgaagcagca aagaaggaat caattgaaac tattcttgat gcattagatt atacatcaca
    22861 gcaaacaaag acctttaaaa aaagattggt tgtattaggc tgtctcaccc agcggtataa
    22921 acaggaaata atacaggaca tacctgaaat agatctggta tggggtttgt acgatagttc
    22981 tttattaaaa gaaattgccc gcttattcaa tattgcactg aatgcaggaa ctatcgcaca
    23041 aaaaagaaaa ccgcttattg ataatcttcc ctatgcatat ataaaaatag cagaaggatg
    23101 ttccaatttt tgtagctact gtgccattcc cctgatacgg ggcaggttta atgcattccc
    23161 accatcgttg attgtggcgg atgcacgtga agcgctgaat cgttatgtta aagaattaat
    23221 tatagtggcg caggatactg cagtatatca gtatgatgat aaaagaatct gggatatact
    23281 ggctatgatt agtaaccttg agggtgattt ctggatacgc cttatgtatt gccatcctga
    23341 tcatattgac gacaggctta ttgaagcact tgttacggta ccaaaggttg tgccgtatat
    23401 tgatattcct ttccagcatg cgagcaaaaa aatcttgcaa tccatgaaca ggcacggcga
    23461 tgcagatacc tatcttaaac ttattgaaaa attacgcgat gccattcccg atattgctat
    23521 acgttccaca ttcatggtgg gattccccga tgaagatgac aatgatttta agctattaat
    23581 ggattttttg caagaagcac agttagatag agttggtgtg tttatgtatt cgcctgagga
    23641 aggcactaaa atatttgcaa aaggggatac ggtgaaaaag gcaacaaaga aaaatcgcta
    23701 cgatatgctg atgaatttgc agcaggaaat ttcacgggaa aggttgatgg aaactctcgg
    23761 tgacacagtg caggtgcttg tggaagaaaa agtggatcgt tataattaca ttggaagaac
    23821 tcaatacgat gcacctgaaa tagatggcat tttttacttg accgcataca atgtacgtgt
    23881 caacgatatt gtgactgcaa taataactga tacattagaa tatgaccgta taggagaaat
    23941 gatttgaata tacctaattt attaacatta tcacggataa tactcatacc attattcttg
    24001 tatttaatct ttacaccaac tatagaacac agaatctggg cactggttat ttttatcata
    24061 gcatcgttta ctgacttttt agatggatgg acagcccgca agttgcgtca ggaaactgat
    24121 acaggaaaat ttttggaccc gcttgctgat aagtttctgg ttatttcagc gcttattgca
    24181 ttcttattcc ttgatccgct aataccgtta tggatggtta ttgttatcat cgcgcgtgac
    24241 cttttgataa cattgatgcg ctatttatct atcaaaaaag gatcaatgtt acgaacaagc
    24301 aggttaggta aatttaaaac agcattccag atgattggca ttatcgttat aataatggtg
    24361 tttattgtcc gtaattccat aaaaaatgta caattggaaa ttaaccagtt aagtgccctg
    24421 aagcttgaaa cagttgttga aattctaaat tcaaatttag ttcataaatg gcttattgta
    24481 tgcccctatc tcattatggc tgtggtaacc ttcttaaccg ctttatcggg attacgatac
    24541 attataacca actggcgttt gtttatacca ccatacacac aaaaggatgc taaataaata
    24601 tatatgaact ggaaagaatt tctgtttaca gggctttata ctggctactt tcccaaaggt
    24661 tcgggtactg ttggtgcact gttagcttta ttattatatg tcatattaca tcagatatgt
    24721 ggcagatatg gggtgttggc caatgttgtg cttgtggcac tggtaacgta tcctgccata
    24781 aagttgggtg atgatgcgga aatttatttt gcacaaaaag acccgcagga agttgttctt
    24841 gatgagatgt tgggtttctg ggttaccatg ttgtttcatc catttagctg gagtacagca
    24901 gttatgggct tctttttttt caggctgttt gatattatta aaccttaccc aatacgcaaa
    24961 ctggagcatt ttcgtggagg gctaggtata atgattgacg atatcattgc cggggtgtat
    25021 gctaacatta ccattgccat tataatggtt attatgaaag cttatgggat tgtacttgtg
    25081 taatgagtac agggtgtcga aaaagaaaat aggcatacaa agaaacattg ctttgaggtt
    25141 agttatgaag cgtacatata caattattgg tgtttttgct attatacttt taacttttta
    25201 tattgcatat gctcaaaaga caatgggcga tagttctcat aaaaaaaatt actgtataga
    25261 atgccatatt aaactcaatg gcaaacctcg tgcagtagtt ctggaatggg aaaacagcat
    25321 acatgcaaaa aataatcttg aatgccatat atgccatggt ggaaatcccg atgcagatga
    25381 tgctaaagct gcaaaggata aaaaagcaaa ttttgttggg aaaccaaaaa agaaagatat
    25441 acctgagttt tgcgggagag aaagttgcca tgatcaggca ctggcacagt tgaaaaaggg
    25501 tcctcactat aattcagttt taaaaatagg tagcccaaac tgtgttgatt gccatggtga
    25561 ccacaatata cagcagtcaa cgtatcacat tattaccgat aaaacatgtt caagctgtca
    25621 tagtgttgag tattctcgtg agctggtaaa ttccattata tcgattgaga aaaatattga
    25681 agatattgaa aaatcgcttg attacatgaa tgaaagaaat gtagaaagca agggcatgac
    25741 acagcggtat aatgagctta aaagttattt tcatcagcta gtgcatgttt tttcaaaaca
    25801 ggaaatagat tttaccaaaa agttagttga agttgaaata gaatatcttg acaaagagct
    25861 acaaagtaaa atagcttcta taaagcgtct tgacctcatt tatattttaa ccagtgcatt
    25921 cagtattggt gtcattatta cgttcactgt atatattgcg ctcatcagac gaaggcgtgc
    25981 aaagatacaa aaggatttcc agtcaaggta aaataaaact tagaaaatac aattatggca
    26041 gatagttatt ttgataggtt taaacgctgg tatcttttca ataccgaaaa aataaaatat
    26101 gttggcaaaa aaaatgacaa gcatgcatgc atactgtgtg caatacgtga ccatgcagaa
    26161 gatgtggtaa gtcttgaaat acatcgtaca gatacattta ttgtttcggt aaatttatac
    26221 ccttttaatc ctggccattg catgatattt cctttgcagc atatagaaca tttgcatgat
    26281 ttatctcttg atgatgtttt agcactgcac agattaacta taaaaactat agatatatta
    26341 aaagaagaat tcaaaccatc agggtttaat gttggataca atttaggttc atggagtgga
    26401 gcaagtatac cgcatattca cctgcatgtt gttccacgct ttcctaatga gttgggattt
    26461 cttgatgtta tatcacatac acgggttatg gtagttgatc cccatgacgt gtgtgaaaga
    26521 ttaaaaaaaa gatttaataa taaccagtaa tttttcctta aaaaataatt tatgataata
    26581 agcaataaac ccacaaaatt ttgacgctat aaatttatac ttcattatat ttattgcttt
    26641 gatgtttctt ctgcttcaag aagtaattct atatcttttt tatttaattt tatagtaaaa
    26701 ggttccccgc acatggggca tgttccgcta ttggcgccta tggaaagttc actaattttt
    26761 aaatttaatt ttgagccgca tagcaaacat gtaaaatcgc cagcacgcat aggatgggga
    26821 agcatataca cctccttgtt gaagtgaaat tgtataaaaa accacttgaa atttataaaa
    26881 tggttgtcaa gtattttaaa aaaatataca atctatatta attcatttca gcacttgacc
    26941 gtaatacata aaacgaggta atacaatgac aaagttaaga aaaataacac taacgttact
    27001 ggtcataagc agcatcattg cattgataat tatttttgac ggagatatcc tgaaaaagat
    27061 ttttgtgttg aaatctgtaa gggttgttac acagagcgag attaagcgta tggaagaaga
    27121 attgtctgaa ctaacttcaa aaagtgcaac aaatcctgaa gtagttaaca aaataggcat
    27181 actgtatcaa aatttaggtg ggaagtataa tgaaaagggt gcctgggacc ctgcaattga
    27241 tgcattacag aaagcattag gatatggaag aaacaaccct cttgtacatt atcagttagc
    27301 aattgcttat gcaaacaggg gaacacagct ggaaaataag gatgacattg ataaagcaca
    27361 aatccattat gaaaaagcat tacaaatgga tcccaacttt atagaagcaa agtatgggct
    27421 ggcaattctt ttgttttatg agaaagataa caggtcaaaa gctgtccaac tgatggagga
    27481 gataacaaag caaaaaccaa ccttttaccg ggcgcggttt gcactgggaa ggttttatta
    27541 tgaaaataaa gatcttaata agtcgctatc ggtataccag gatctttata ctgatttaga
    27601 aaaaatggat tcaccgttag caaaggaata taaacaggcg tgtaaagaaa atattcaacg
    27661 aataactacg gagttagcac ggtaattaat atgaacaata agaaatatgc tgtagcgtta
    27721 tcactgatga gttctggtca ctggcatttc tattctaaaa gtgatccgga aacaatttat
    27781 aatgaatatg ttttgccaga ggaaagaaag gttcaggaat actatacaag ctggtattca
    27841 atgtcaccgc ttgaagaagc agaaaaaata attgagttat gtaacaaaat gaatgtgaac
    27901 atgatttcat actgggatga ggattatccg gctttattaa aagaaatttc atatcctcca
    27961 gcggttctat atgtacgagg taacctgcct aataaattat cccttgcaat tgttggtacc
    28021 agaaatgatg atactcagtc cggttctata gctgaaaaat tggcaggtat tttagcacag
    28081 tatgatattg ccatcgtaag tgggcttgcc attggaattg atagaagagc gcatataggt
    28141 gcattaaaag caggtggatc aacaataggg gtcatggcaa atggtattga tatgttgtat
    28201 ccggcaagta accgggatat ttacactgat attatcaatt caggcaatgg atgtgtgtta
    28261 tcagaatatc caccaaaggt gcgtatcacc agatggacat ttgtacgcag gaatagaatt
    28321 atcagcggat tatcaaaagg ggttattgtc attaaagccg gtgaaaaaag tggagcactt
    28381 attactgccc agtatgctct tgaacataac agggaattat ttgtttgtac tggacattct
    28441 tttgatgaag gatacaaagg gtgctataag ctgttaaaag aaggtgctca tccagtattt
    28501 aatgaacagg atatcctgga aattcttagt gttgataaat tatacaggca accaacatta
    28561 tttgaaggta tggagtttaa taaagttact tcaaatacac attttgctgc gtatgaggat
    28621 gatgatattg tcaaaacact aaaacaaggt tcaatggata ttgattcact tatacgggca
    28681 gtgggaaaaa acagtggcga agttcttgaa aaattaacca ctatggaatt agagggcgtt
    28741 atcattcgta atgggaacct catatcgttg aataaataaa ggtttttatg ataattaaac
    28801 gagttcttat aattatgtta gcattactgt gggtaataag ctttattcag attttttctg
    28861 tgccaccata tattggcgat atatttgaga tatataaatc gggctatttt aaagaacttg
    28921 aaagaaatat gtatataata accgatagtt ttttacacat taaaacaatg acaaaaccag
    28981 aatatgcctg gatatacctg gatgatttgc aaaaacgttc aggtatcagc attgcggtgt
    29041 atgatgcaca gggtaattta attaaagcgc cgggtatgtc cgagatagtt aacaatactg
    29101 agattatgaa tgtatgtaat gatattaagc cacaagcgca atttacagta tcaggaaggc
    29161 tttaccaagg catcataccg gtttaccgga aatcagaatg taatttttgt catcagcctt
    29221 cgcaaaaacc attattgggt gtaataacct atgcaatccc ttttgatgga tatatttact
    29281 atacttctga acgaataata ctttttacca tcattacatt tgccataagt attatgcttt
    29341 ttgttgttat cagatggaat ccatataagg atattaagga actgtttgac aaaaaataat
    29401 atacattgaa ttcacagtat cagtaactat cgcttaaaaa ttagaaagta aggaagtaag
    29461 tatggctgat cataagaaaa cccttgtgat tgttgaatca cctacaaaag caaaaacaat
    29521 acataaatat ttaggaaaag attatattat agcttcgtca atggggcata tcattgattt
    29581 gcccaaatca cgtttggcag ttgatgtgaa taatgatttc aaacctgaat atatccctat
    29641 acgtggtaag gcaaagttaa taaacgactt aaaaaagaaa gcaagtgaag caaacaatgt
    29701 tttacttgca actgaccctg accgtgaagg tgaagccata tcatggcatc tatccaatgt
    29761 tttgaaagaa aaaaatccag atataaagcg tattgaattc catgagataa ccgaagatgc
    29821 aattaaagaa gctgttgaaa aagcccgcga cattaatatg gatctggtca atgcacagca
    29881 agcacgtcgt attttagaca ggcttgtggg ttactattta agcccgatat tatggaaaaa
    29941 ggttaagaag ggattatccg caggaagagt tcagtctgtt gcgttacggg ttatatgtga
    30001 acgtgaaaaa gaaattgaaa attttattcc tcaggaatac tggacgcttg acgcgttatt
    30061 tgaacatcat aagcaagaat ttgtggcatc acttgtttca gaaaataaaa ataaaattga
    30121 actaaaaaca aagaacgatg ttgacagggt attaaagctt atagaaggca aaccgtttac
    30181 cgtaagcaat gttgaacaaa aagaacgaag aagaaagcct cagcccccct acattaccag
    30241 caaattgcag caggatgctg caaggcgttt aggattttcc gcaagtaaaa ccatgatggt
    30301 ggcacaacaa ttgtatgaag gtgttacaat atcaggtgaa gggcaggttg ggctaattac
    30361 atacatgcgt accgattcaa cgcgcgtggc tcctgcagca ctggcttcag tgagggaata
    30421 tcttggtaaa aatttttcta aggagtatat tcctgaatcg ccaaattttt atccaaacaa
    30481 aaaaggtgca caggatgcac atgaagcaat acggccaacc aatgtgttca ggactcctga
    30541 atcaattaaa aacgatttaa cacctgacca gtacaagctg tattctttga tatggaagcg
    30601 atttgtttct tcgcagatga cttcggaagt ttctttagta gttaccgtta ccatgaatgt
    30661 tgaacatttt gagttcaggg caacaggaag cagagtattg tttaatggct tcactgtgat
    30721 agaaaaagag gaaagtgctg aaaagaagtt actaccacca ctggatgtgg ggcaggttct
    30781 tatcccaaaa tcatttgacc cgcagcagca ttttacttca ccgcctccac ggtacaatga
    30841 agcttcgctg gtaaaatttc tggaagaatc aggtataggc agaccttcta cgtatgcccc
    30901 aaccataaat acgctgttaa aacggtatta tgtgacaaaa cgtggtaaac agctggtgcc
    30961 cacggtttta ggaagactgg tcaatgacat tatagtctca tattttgaat ctttgataaa
    31021 tattgatttt actgcaaaga tggaagaaaa attagattta attgaagaag cacacaatga
    31081 ctgggtgagt atgattaagg aattttacga acccttcaag ttaaccgttc atcacgccga
    31141 agaacatatt gaagatatga aaaacatact ggatgaagaa actgatctgg tctgtgaaaa
    31201 atgcggtagt aaaatggtta agcggttagg gaaatatgga ttctttttag catgttcggc
    31261 gtttcccaaa tgttctaatg caaaaccttt gcctcttggt aaatgcccaa agtgtggtgg
    31321 cgatgtaatc aaacgtacat caaaacgtcg cggctcattt tatgcatgta acaggtatcc
    31381 tgaatgtgat tttgccaccc gtgaacaggc atcagaacac gcatgcccca aatgcgggag
    31441 tctccttttt atcaggaaaa taaagaaaaa aggcgagatg cttgtatgtt tgaatgaaca
    31501 gtgtggctat tcagtaacac tgttagacga agataaaaca gatgcggagg cacatgtaag
    31561 ctgatacgta tggatcacta tattgataaa ttcatgcaat atcttattgc tgagaaaaat
    31621 gcttcacctc ttactgttga atcatatggc aaagacctga atcaattcat tgaattttta
    31681 tatgaagcat atgatcctga atcagttact atcgccgcct taactacaaa tgatatacgt
    31741 gattttattg attattgctt tgaaaaaggg aatgaacttt caacaattga aagaaaggtt
    31801 gctacgttaa aatctttttt taaatatctt gaatttcata actatattga taataatcct
    31861 gcccgcgaca tccattactc caggcaaaaa aaatttttgc caacattttt aacctttgat
    31921 cagattcagg cgctggttgc gtttgaatgc aaaaatttta gtgactatcg cgacaaagca
    31981 ttgcttgagg tgttttattc cactggggct cgcgtttcgg aaatagcaaa tgctaacatt
    32041 gatgattgtg atttcatttc aggaagatta aaggtgcttg gtaaaggatc aaaagaaaga
    32101 attgtattta tgaatgaaac ttctgtgacg tcattgcaag aatatttgaa tgaacgcaaa
    32161 aaaaaattca attgtttaac cagtccctta tttgtaaata ataacggcaa acgattgaca
    32221 acacgaggta ttttttatat tattgataaa agagcaaaca tggcacagtt atggaaatcg
    32281 gttacaccgc atgtgttgcg ccacagcttt gcaactgaat tactcaacag gggagctgat
    32341 atcagggctg tgcaggatat gttggggcat gagagtattt caacaacaca gaaatatact
    32401 cacaccacga cagagcggtt aaaagcatta taccagaagt atcatcccca tgcacacagg
    32461 agacatgata atgaataaca cttcaatgcg tggcaccaca gtactggcaa tcaaaaaaga
    32521 caatactatt gccatgggat gcgacggaca ggttacgtta ggtgatacag ttattaaaag
    32581 caaagcagtg aaacttagaa aagtctatga tgataaaatc ctcacgggtt ttgcgggttc
    32641 ggtggctgat gcgctgacac tgtttgaacg gtttgaacaa aagttagaat cgtattcggg
    32701 caatatcccg cgaagtgtgg tggagcttgc acgcgactgg cgtttggaca gggcattgcg
    32761 cagacttgag gcggtgttga ttgttggcaa tccgcagcac ctgtatttaa tcagcggcaa
    32821 cggtgaaatc attgagcctg atgatggcat tattggcatt ggttcgggtg gaagctatgc
    32881 gctggcagca gcacgagcgc tggtacgcaa caccacgttg aatgcgtacg atattgtgaa
    32941 gcagtcactg cttattgcat ccgagatctg catctattca aacagcaaca ttataattga
    33001 ggagatcaaa gcataatgag tgataatact attaagtttt ccgaagatgg tgatttgact
    33061 ccacggcaga ttgtcagtga gttagataaa tatatcattg gacaaaaaga tgcaaaaaaa
    33121 tgtgtggcga tagctctcag aaacagaagc cgcaggaaga aactggatcc tgagctacag
    33181 gaagaaattg cacccaaaaa tatcatcatg ataggaccca caggtgtagg aaaaaccgag
    33241 attgcacggc gattatcacg gctggttaat gcgccattta ttaaagtgga gattacaaaa
    33301 tataccgagg tggggtatgt tggcagggat gttgaatcca tgatacgcga tttaacatcc
    33361 attgctgtga atatggtcaa gaaagaatat ggtgcaatgg tagaggagaa agctttaaaa
    33421 aatgcttatg ataagatagt tgatatcatc attcccaaaa aggggtttga atttgatgag
    33481 cggcagattt caggttttca tgtagaaagg gaatctattt ttgagtccga cacattgtat
    33541 gagcagatgc gcgcacaata tttagaagat ttaaaaagcg gtaaattgga tgatcttgag
    33601 attgaatatg aatccgaatc acccaatgtt ataccggtta tgtcaattat gggtggttct
    33661 ggccttgaag atatagattt gcagattcaa tccatgctgg gtgatttgat gccaaagaaa
    33721 tcaaagcgaa aaaaaagtac tgttaaagat gcattgcgta tattcattgc cgaagaaacc
    33781 gaaaagctta tagatatgga aaaagttacc aatgaagcca tacgacgtgt tgaagatatg
    33841 ggaatagtct ttatagatga aatagacaaa attgccggtg ccgaatcaaa gacaggtccc
    33901 gatgtatccc gccagggggt acagagggat ttactgccca ttgttgaagg cacaacggtc
    33961 aatacaaaat atggtcctgt aaaaaccaat catatactct tcattgcagc aggtgcattc
    34021 aatatttcta agccttcgga ccttatacct gaactgcagg gacgtttccc aatacgggta
    34081 gaactgtctt cattaaccga ggaggatttc aggaaaatat taacagtgcc aaaaaattca
    34141 cttacccttc aatacaaaga gcttcttaaa accgaaggcg ttaccattga ttttactgat
    34201 gatggtattg ccgaaatagc aaagacagcg cagatactca atgaagagca tgaaaacata
    34261 ggtgcacgta ggctgcatac cattatggaa aagctacttg aagacatact ctttgatgca
    34321 ccggatttag atgatgccca taaaaatttt gttattaatg ctgaatttgt caaagccaag
    34381 ttgggaccaa ccataaagaa taaggatctg tcacggtata tattgtaaat atacattagc
    34441 catgttatgt tatctgcaca cctgaatcaa gtattacctt cctttgtgag gcttcaggtt
    34501 attttgattt tatccctggt ttataatttt tacttaaatt tcattaattg aatactgatg
    34561 attttttgac ctgcttcatc caataattaa agtaatagat aaaaaatatt attgacataa
    34621 taccataata tgttataata ttttaattca ttgtctaaca atttagattt taaaaaaaat
    34681 attaggtggg acattatatg tccctgcgga attttatact ataaacaaaa aatataagga
    34741 gtttttcaat gaggtttgca gcatttttgg aatttgaaca gccagcatac attcccccat
    34801 ggtaccgaat agcatttacg tcattattaa aagaagcatt gcagcacaat aaggaaacac
    34861 agtctctata tgaatattat tat
//
LOCUS       DAORYL010000024        33325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1078_length_33325_cov_12.340697, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000024 DAORYL010000000
VERSION     DAORYL010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 33325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33325
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1078_length_33325_cov_12.340697"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(272..1444)
                     /locus_tag="PLV81_06420"
     CDS             complement(272..1444)
                     /locus_tag="PLV81_06420"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOM09773.1"
                     /translation="MLGKRIKVDVNYLRPNSTFIYPLISEDGDVVLPPRVALTAEKIN
                     AIKARYGSILYYNDNGQMAVIPTFRMQIARNKSKEIMHEIAQSHKISKVAIHEAEKVI
                     IDIVNDLTSTEILALNLLKDLKSYEEYIYNHSVNVGILSAVMARMIGSFNKDEIKNIA
                     LGGYLHDIGQMQLDKNLLEKEGKYNITDIQKMKRHPQLGYETLKKIKDVHPIVLQSVL
                     FHHERYNNNGYYGLPYENLPIYPKIIGLCDIYDALTSKRPFRDAIEPSNALKAILNAI
                     NQQFDYQLINIFINKLGPILNNTQAFYTHNDICELNTQELAIIKDFGINDMLKPKVII
                     FCKFQRTGEQLLARFYEKPLEIDLSQDPNNRYMIKIINNYKQLQSIQKKLEEKKLL"
     gene            complement(1712..2257)
                     /locus_tag="PLV81_06425"
     CDS             complement(1712..2257)
                     /locus_tag="PLV81_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09774.1"
                     /translation="MAKGTALFKAYMNNTIPSNGGFIICSFFDADSIYSIYEVTAYKN
                     VKDIYQTAEGLTFKTDGSRTHLLVEPPTYARRYEEPVHREAGKSIPYRFDDLTIITGQ
                     KQEKIMIPKEPVMLYTSFTILKTQGENFAFLFYPTEDVYMAIRKFMADSLYNDCGLTK
                     EDALNAAQILLQTIKNFTVFT"
     gene            complement(2493..3101)
                     /locus_tag="PLV81_06430"
     CDS             complement(2493..3101)
                     /locus_tag="PLV81_06430"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOM09775.1"
                     /translation="MATISQLLFDFAPHVHYIAFILLLLAGFNFPISEDIIFIISASI
                     AATIIPEHTFKIAAGCVLGAYSSDIISYCLGRFGMNFILQHKKLAKFYPLDKIQKIEY
                     YYQSHGAKTLFFGRFIPFGVRNVIFLTAGLAKMKLYKFMLVDFLALSITSTILFTIGY
                     TLGSNYEIIFPYLNKYKLIIFSIFLVTVLSLVIYNKKKKSPY"
     gene            complement(3125..3451)
                     /locus_tag="PLV81_06435"
     CDS             complement(3125..3451)
                     /locus_tag="PLV81_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase Ppa"
                     /protein_id="HOM09776.1"
                     /translation="MEEQIKLLNLLKENDKTAIDKYRNINFFENHVAFVGTPKKHQYD
                     KTKIILLSDPFSDKKIFYEFSIDTIDLVEELGTISSLDGKNALQIRIWVKKGTVAIKY
                     EPFIIE"
     gene            complement(3470..4063)
                     /locus_tag="PLV81_06440"
     CDS             complement(3470..4063)
                     /locus_tag="PLV81_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOM09777.1"
                     /translation="MPQIVKPETKTKILYAAEKLFNEKGYSKTKITDIMDYCNLATGT
                     FYVYFKDKEMLLTEIYQPIVDSIDSIFILSPLPEHITQAEYFIILKQEILRLSEFALK
                     FTSELGFLLLHAYDSKFAYFPEKLQHIIQQELEKFLTIGISHGFIRNAHIPILSKVCC
                     GIITTMINLLIIDSYKPLQGIIVQETIEIIINGIKKI"
     gene            4214..6478
                     /locus_tag="PLV81_06445"
     CDS             4214..6478
                     /locus_tag="PLV81_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09778.1"
                     /translation="MKKLTGKIHPQHLPAFIIAIAILLSFIIIQKIYFTILLNQKITP
                     QVIEEIFENNFKKAIQFDSSSVTLGGNIVLKNVKIAPTTDFNDNYNLISCKKAIIDLN
                     YIKAITGSMVIRGVTFTDASMTIVKNYGKSYLETFKGIFSGIVNGNIINLDDFYMELD
                     GNLLYNENLSADKLNLSIQDVSLAISMKNKHLSYTIKGEVLPTDSTLDSGRVHISGII
                     DYTDAMGYKASHHSIYAKKLDMHIVNYFLKEYSEYPITVRGYFYTDCTIDHDSAYSFK
                     GTVEFDNCTIENMKNIPHFEYISKDNISIDALMECNEDLSEINVSSFTFNDKEISIDA
                     TLKYSKDKLLQFYFSTNQIDLDDCDYIQLVPGLYYDGSLKIMTQCDFDIANDTMKSFK
                     ILCDAHDVSVFKKVNNATTSYLKSTRLKVEGDVKSVILDFFAKHKKSDILIQSKINID
                     KWAPFSSTTDITITSKQLHSDVLVYLVADGISSLYTGAMEDMGLGYNEIFFRDKPLGK
                     YLINNIIKLKCNIGKLLFDNNAHLKNIKIDILSNKGTISTPSFNCEGYSGIYSFNINA
                     FCNCDYPNLTITAGVSNFDYGRYLQDCGQKDATGVMNVSLNYSLSGYRLSHLLQNGNG
                     LLQVTIANTIFDKTLLQKKIGEMAKICNISFADGVWRCNRLDLTINHIADRWILQNMF
                     IDTDMVQLGGYGNYTIDKGLSLPCYATVFTREGPVIKGSQRINFVITGTLDNPVIATG
                     TPCGKKEISLFDVN"
     gene            complement(6458..8446)
                     /locus_tag="PLV81_06450"
     CDS             complement(6458..8446)
                     /locus_tag="PLV81_06450"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOM09779.1"
                     /translation="MQINKNQFLGLVLSVVVFIILYFLYTQTNLLDGLEYGSVNFRFY
                     LRDPSEKAVKLQEGVRMSRKNPRSRDDIIILGIDENTIREFDNEGIKWPFPWNYHTKF
                     TRYVGSGEPKAMFFDIMFLDHKPYEEEFAKAIKEANCVFLDYPFETEEIDVKYTDIEE
                     RMRILDIYSFPVDPQDNSVPWVEEAVPPTPLLSQAAIGLGFANIRPDADHVNRKVPLV
                     IKYKGRYYPSIDLLIVMHYYGITKKDITISMGNYIKLSNIPRDKMAKPTPDNSITIPI
                     DDEGFMDINFIGGPGSFTHYPYYYFCRDGKIQNTSLKDKIILVAAYASTGISTDIHKS
                     PYGDLFGIEHHANALNTILNQDFILKFTPWQNLLILLFISLLLGFILTQVSIVVSVAF
                     TLGLVLFYIIGSYLLFDIFNIITIFATPIIQVGTTFSIIIAYRVLTEQQEKKYIRQTF
                     SKFVSKTVVDELLKHPDKLKLGGDKKILTVLFSDIRSFTTISEKMTPEELVEHLNEYL
                     QAMTELVFKYDGTLDKYVGDEIMAFWGAPIPQDNHALLACKCAVEMMQVLEQLNKRWV
                     QMNKPALHIGIGINTGEMVVGNMGSASRMDYTLMGDNVNLGARLEGTNKQYGTGIIIS
                     EFTYEHVKDHVIARELDLVRVKGKQLPVKIYELIDIKE"
     gene            8665..10323
                     /gene="argS"
                     /locus_tag="PLV81_06455"
     CDS             8665..10323
                     /gene="argS"
                     /locus_tag="PLV81_06455"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOM09780.1"
                     /translation="MKEYIAQTVGQALTKLLQEKNISTELSPAIKVEYPKEEKFGDYA
                     TPVAMECAKILRMSPIQIGEEIKQHINADVFDRIDVVKPGFINIFLSLPFLLQRLEEI
                     VTVKDEYGKNKKSNPRKVNIEFVSANPTGPLNVVSARAAAVGDTLANLLAASGDTVHR
                     EFYVNDYGNQVLWLGKSVWIRYRQMLGEDVPFPEEGYHGEYVKDIAKYIHENFSQELS
                     HINNEEEIINFCAQKAIEYNVASQKEDLDKFNVSFDTWFHESTLHHSGKVLDVFKYLE
                     SLGVIRDEDGKKVFVSTQFGDDKDRVVVRDDGRPTYLMADIAYHRTKIERGYDLIIDI
                     WGPDHHGYIARLVGAMKAMGYDEKRFRILISQQVNLLMNGEAVKMSKRLGTFSTMRDL
                     IEEIGTDVARYFFVMRSMDSHLDFDIDLAKRQSSENPVFYLQYAHARICSIFREAQNR
                     GIEYKKIITNTEYFNNQETIALLKLMARFPEEVYDAASSFEPHRITVYCMRLAQAYHK
                     FYTEHRVLTDNEDRTQSYLTLCDGVRIILKNALALLGVSAPERM"
     gene            10414..11631
                     /locus_tag="PLV81_06460"
     CDS             10414..11631
                     /locus_tag="PLV81_06460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HOM09781.1"
                     /translation="MKASLIATGSELVCGRVHESNNFYISQHLLAIGLPVMMHYVVGD
                     DSETLRLAIEHALHSADIIIISGGLGPTVDDITFETLEKMFALKPVIHEPSKERMEYF
                     FNAIGKSVLPSDFKMVTVPENAVVFVNDAGLAPGFAITVKEKDIIVLPGVPRELHSMI
                     GKVIDYLESHYQLKKKNSVTFKVVMMREAEVDSGVKQIVNSIKGVDVSITYEAGMTTV
                     MLTANDESILPVDSIVDKAKELFGSKMVYGYNSLEEKVVKVFASHGKTIAIAESCTGG
                     LIAKRITDIPGSSNVLLGGIVAYSNQSKISMLGVNPDTLQTYGAVSTETALEMAAGAK
                     EKFNADYGISTTGIAGPDGGTKDKPVGLVCFGIAGSNTFTTQIQFAGNRERIRTMATN
                     YILTRLLTCAGLE"
     gene            11628..14444
                     /locus_tag="PLV81_06465"
     CDS             11628..14444
                     /locus_tag="PLV81_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM09782.1"
                     /translation="MKRVFILFTILLFFNAASYAIESSQLFEQGMEAFRMGNYGSSEL
                     IFRKVIDANDSFKDRAWYYLALSIFYQKQYRSAIFELNRFLLLCTTPDLCSEARYWIA
                     ESYYYLNDYIKAIEEYNRFISQSKNKQLIINSYDRIGEIYFNQKRYDEAIIEWKKALN
                     ISSKKYEDSNRVIKIGEALFLSERYNEALDLLEPLIYATGDNTIQSKARLLVGRIYQL
                     TGKHWQAIKYFNEIPESMLRVPSFISVQYFKALSYIALGDTYNARLNLKSYILISADT
                     VQYHYNAKYELGKILLNTRDSDEGMSLLEDVYTNSNDIDLRSNASFELAKVYLKNNNN
                     EKAIPYLENAVSINDAESQKYVMMTLTRLYITTARYDDAKRLLDFMLEKYQYEENRDE
                     MLFLIAQVYVMQGNITKALEVFEDIKEINPFSQYLVELDYVKAIDAFKKEQYNDTIDY
                     GNRYLKNQKIENRYEVLILLAESYIKINNTKKTIEVIDQLITRFPKKAGLEEILYELC
                     IVLKEQGINVERYYSFIIKNYTSSITAGKIFIEKGDEAYKKKNYQVAVYFYSQYLATK
                     GRPRERSVLLFYILSLYELKQYTTIIKTLQDSDYLTVDDLTTKLLIKMRGKSFYALKD
                     YPAALSDFMKIPFQDYNKEDFVIIADTALKASQPEIAIDILPYVEDDEYFAVISHSIA
                     LYYRINGNNDLAFDYFTKALLRKPDSSVSDDTKIRLAQIYIERKDFEKALDLAKSVQK
                     KELKNLKNSIMAVTYFNLGNNDKALTVILGKLNDILKTEYAEEIITFLLRHYYIQGDI
                     KNYQTIYWYGIKRNKLISLSNHYYGMMMYKNNNYSRALQFFNTASRYDDEYAHASLYY
                     AGLIYLLIQRNYGIAKKYFDLYFSKAGSTDINFYNAKVYLSLLLYETGKQDESKDILY
                     EIINSDSNVLAKIKADHLIKQYGF"
     gene            14473..14922
                     /gene="aroQ"
                     /locus_tag="PLV81_06470"
     CDS             14473..14922
                     /gene="aroQ"
                     /locus_tag="PLV81_06470"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015955457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOM09783.1"
                     /translation="MKQYTFHVIHGPNLNLLGEREPAIYGTLTLEEINNRIREFAKSK
                     NCEVRCFQSNSEGEIIDYIQKNRNCDGIIINAGAYTHTSIAIRDCLVSSGLPCIEVHL
                     SNIHSRERFRRHSYIAPVCIGQICGFGYYSYIIALQALIHYLENYSK"
     gene            14933..16009
                     /locus_tag="PLV81_06475"
     CDS             14933..16009
                     /locus_tag="PLV81_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOM09784.1"
                     /translation="MNYRTRIKKIQKHLSEKKQFPYLVASLINIKYCTGFEGSYGYLI
                     FFEDETVFISDSRYEEYALMILPDTVRFVLQQKDFFTSLKSLLKEKKIKKIFLEEHSI
                     TLSQYKMLKNECKGIAFNYGEDVINELRIIKDEDEIETIKKAAQITDACFNHLLKIIK
                     PGVTEWDIAIEIEYFYKKHGCQGTSFDTIVASGAGSSMPHYKTGTKKIKAGEPVLIDM
                     GCIFKGYCSDITRTVFIKSIPDSVNTIYSIVKEAQKRAVDAVKAGLTTNKLDSIARDF
                     IAANGYGDLFGHSLGHGVGLDVHEIPAVKQNGAMVLKKGMVITIEPGIYIPKKAGVRI
                     EDMVLVTSSGHEVLTKSTKELIIL"
     gene            16036..16599
                     /gene="efp"
                     /locus_tag="PLV81_06480"
     CDS             16036..16599
                     /gene="efp"
                     /locus_tag="PLV81_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOM09785.1"
                     /translation="MITSNDLRTGTVIKVDGDLYSVVEYQHVKLGRGGAFVQTKLKSL
                     TKGVIVDKKFKGGEKVEDVRVEKKTLQYLYDEGDSLVFMDTESYDQIHVDKKKASNLL
                     NYIKEGDSVEATMHDEEILSIEPPIFVVLKVTYAEPGVKGDTATNVLKPVTVETGATV
                     QVPIFVNEGDVIKVDTRTGEYVERVKK"
     gene            16855..18006
                     /locus_tag="PLV81_06485"
     CDS             16855..18006
                     /locus_tag="PLV81_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09786.1"
                     /translation="MKSEIITKEAYKAFKKGSYVVVIQLLEKNITISNNPYDYFLLIL
                     SYLFTDKLMQAEAALRKALRQYPAYLPLLEIQAFLMLKSAKSKDEACTGYIDILKISP
                     GNKQITKIIAKIQESADFEQFQKKAKLRDFIKVKAPRTIKTVSFRARRPTIHYKTVAL
                     IVIIIAFLITGIIFFPSPLAKLIQEKIELLFFHPALQTGEDLSQISLDGMHYDIIDKI
                     QKVRTPEFYASNEACIQDFNQAKWLIKEGKENDAMILLNKIDASNANFRVKERVAFLK
                     DFIHRNRERTVAKFSYRDVIQKPYLYKGINVKWQGKVANYKKKDGINFQLLINYSENR
                     FDGVVEVFAPFNVQALDNGAIVTVEAIITNIVGQKIVIMQATNVEIKSE"
     gene            18048..19187
                     /locus_tag="PLV81_06490"
     CDS             18048..19187
                     /locus_tag="PLV81_06490"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOM09787.1"
                     /translation="MKVIVNECSHYDVEAIKTKVIAALNKLSFDFSTLYNAKVALKPN
                     LLTSASPESAVITHPVFFKAIVQIVKQYGGIPVLVESPAVHSLQRVMKKTGYDAIVTE
                     EDVLVADTSDYIVIHNDAAKHFKRFEVPKILTECDILINIPKFKTHALTHITCAVKNL
                     FGTIHGMKKSQWHIKAKTKDEFAHMLLDLYQAYCTDTAVPKTIVHIVDAITMMEGDGP
                     GPSGRPAFLGVIGVSYNAIAVDYALSQLAGFSIDNIPTIIIGFKRGLCSIPQNIDIIK
                     DSEISGRISITPKEAGSTKILGVPIINRILKNLLVAKPEPDPEKCTLCYQCKQICPVK
                     AISTSKDGKVPWYDYSVCIRCYCCMEICPESAISLTKPLLQRILK"
     gene            19321..19701
                     /locus_tag="PLV81_06495"
     CDS             19321..19701
                     /locus_tag="PLV81_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09788.1"
                     /translation="MKPKDTIRKIIVIEIVVFLIIIVFIWAEEIFDLPHLLLNAEPTP
                     VNYEESLIETFIFTAIFGFLVYHTIRLLIRLRNLESYVRICAGCNKIYVEGRWVTLEE
                     YFNRYANKKTSHGLCDDCRKYYKK"
     gene            complement(19703..19776)
                     /locus_tag="PLV81_06500"
     tRNA            complement(19703..19776)
                     /locus_tag="PLV81_06500"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19740..19742),aa:Met,seq:cat)
     gene            complement(19778..19853)
                     /locus_tag="PLV81_06505"
     tRNA            complement(19778..19853)
                     /locus_tag="PLV81_06505"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19818..19820),aa:Met,seq:cat)
     gene            20449..20856
                     /gene="speD"
                     /locus_tag="PLV81_06510"
     CDS             20449..20856
                     /gene="speD"
                     /locus_tag="PLV81_06510"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HOM09789.1"
                     /translation="MEGLGTHIIAELFNCNEFHINNLKKVEEVLTAAAELSKATIIQP
                     FFHKFSPYGISGVIVIAESHITIHTWPEYGYAAVDVFTCGDHNYRVAIDYITEQLEAE
                     RCVLYEMRRGVLNGSQVKNVPVIREVENVNYID"
     gene            20903..21253
                     /locus_tag="PLV81_06515"
                     /pseudo
     CDS             20903..21253
                     /locus_tag="PLV81_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770061.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
     gene            21369..22235
                     /gene="speE"
                     /locus_tag="PLV81_06520"
     CDS             21369..22235
                     /gene="speE"
                     /locus_tag="PLV81_06520"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HOM09790.1"
                     /translation="MPVESSVWIEELYEDEHGRTMKLKVTNRLATYDSKFQRIEIYDT
                     KAFGRMLVLDGVFMTTEVDEMGYHEMLVHVPMCVHPNPRRVLVIGGGDGGTLREVLKH
                     KEVEKADICEIDGDVIRLCREYMPALSCSFDDPRVTIYTEDGAKFIAERKKAYDVILV
                     DSSDPIGPAAVLFSEEFYKNLSECLDDDGIASTQSESIFYHPNVIITLAQYNKKFFKV
                     PSYYYTMVPTYPSGMIGFSFCSKKYHPINDLKEEKAKALVKQLRYYNPDIHKGAFALP
                     QFIRKKLEGILY"
     gene            22242..23825
                     /locus_tag="PLV81_06525"
     CDS             22242..23825
                     /locus_tag="PLV81_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOM09791.1"
                     /translation="MSAIDWIIVVLYSICVIGIGYVIGKKQKNQADYYIASQKASAWL
                     TGLSLAANQVSAISLIGVPAFIAIKHNGGLTWLQYELALPLSMMFLILFVVPAFRSFQ
                     GFSIYEWLEKRFGKHTRVILALVFLLSRSLATGVAIVATSYVVSVLIGIDLYISIISI
                     SLISLLYTSIGGILADIYTDVMQLVVLLFSTVLFCGIIIYAIDGHTSFTIAYHRIKIF
                     NYASFGIQDGNTFSLAPMLCGGFFLYISYYGFDQSQAQRFLSCENTDAAQKAIIINTI
                     VRFVIVMLYSFLGILLLLLLSVDNTLRSLLTNQQPDMLIPTFIKHYIPAGLKGVVISG
                     ILAASMSSMDSAINSLSAITYNDALKIAYEKVESIHDKTKLLLSRLLTVFWGVITTLF
                     AIIFAGSSETVVELVNKIGSLLYGPICAVFIIGMLTRKKIHQLAVTGGFATGILVNIY
                     IWLFLPSVSWMWWNATGFFAAVLLPFIFSFFTDSEYISDSGYTGKTLPKNTWFKAIVL
                     GIWFMAIIGFFMIIEKVFE"
     gene            complement(23883..24578)
                     /locus_tag="PLV81_06530"
     CDS             complement(23883..24578)
                     /locus_tag="PLV81_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOM09792.1"
                     /translation="MYQYPLGTYLTHYTVNKLRNIKPHFLCFPEYFFVNKKLGNHVQT
                     QHNQSLQLKRMQVLSKELNTVVIGGTMPELNGNYLHNTTFVFHNGTMLGFYRKQNLFF
                     AEEGKITPGNSHVIFTAYGIRFSVLICADVFKDEHFIALKELGAQIIFIPTFSPIRNE
                     TIEEKFKRDQDIFVRGAQLANAILVKVCGVKSDYKNFLQARSLIASPHGILYRVEPDQ
                     EDKAMLIVNTVSL"
     gene            24800..27352
                     /gene="glgP"
                     /locus_tag="PLV81_06535"
     CDS             24800..27352
                     /gene="glgP"
                     /locus_tag="PLV81_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOM09793.1"
                     /translation="MSTIQTFRVYPYLPDNLKPLKTIAYNMWWVWNFEAIELFRRLNV
                     ELWRESNHNPVALLGMLSQSELDEAADSESFMAHLKRVSEELEWHLTKKSWFEEAYPT
                     YKDMQIAYFSAEFGIHECLPIYSGGLGVLAGDHIKSASELGLPFFAVGLLYRLGYFHQ
                     YLNIDGWQQERYPETDFHNLPLQLVMKDDKPLIIDVEFPGRNVYAQVWECIIGRIRLY
                     LLDTNISENSIEDRTITDQLYGGDVEMRIKQEMLLGIGGYRMLKALGKNISVYHMNEG
                     HSAFLSIERIYDAMVTHKMSFNEALEFVTAGTVFTTHTPVPAGNDRFSPELIDKYLSH
                     YYQKLGISREEFLGLGRENPHDKLETFCMTVLAIKTAAHCNGVSKLHGAVSRKMWRNI
                     WPGLPVNEVPIHHVTNGVQILSWTSDEMMRLLNRYLGPRWIDNPVDKTIWKNIDTIPD
                     SELWRCRERLRERVVAFARKKLREQLIARGAPRAEVEKADSVLDSEALTIGFARRFAT
                     YKRATLILKDMQRLERILLDKERPVQIIFAGKAHPRDNMGKELIKRVINLGRDDRFRS
                     KIVFLEDYDINIARYLVQGVDVWLNTPIRPQEASGTSGMKVVPNGALNISVLDGWWPE
                     AFNGNNGWAIGKGEEYQDPEYQDEVESLSLYNILEQEVIPQFYARGADGIPREWVRKI
                     KESMKTILPQFNTNRMVKEYTEMYYLTAYDSYVKFSKDNFKASRELEKWKLNIRQKWQ
                     QVTVDSISHESKKEIIVGSQLKAKAVINLGELKPADVQVELYFGNLNQKGDIEEGAAL
                     PMLMVEDLGNGKYTYEGRMLCLKSGQFGFTVRVIPLHADMPRKYESRMITWA"
     gene            complement(27397..29199)
                     /gene="lepA"
                     /locus_tag="PLV81_06540"
     CDS             complement(27397..29199)
                     /gene="lepA"
                     /locus_tag="PLV81_06540"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOM09794.1"
                     /translation="MYMKQSLIRNFSIIAHIDHGKSTLADRIIERCNAIDPRNHVDQL
                     LDDMDIERERGITIKANVITLNYPSKDGNIYTFNLIDTPGHVDFTYEVSRALAACEGV
                     LLLVDATQGVEAQTIANMYLALENDLTIVPVINKIDMPNADIPRTKESIKKSLGLDPE
                     TAICVSAREGTGIDDLLEKIVERIPPPQGNPDGPLKALIFDSYFDQYVGVVTKVRIFD
                     GTVKEDDIIMLFSNQKQYEVTEVGHFRLKREKRSQLTAGEVGYITAGIKQLTDTRTGD
                     TITSAQNPVAEPILGFREAKPMVFSGLYPSISDDYDDLKEALYKLKLNDAALFFEPDN
                     SYALGFGFRCGYLGLLHMEIVQERLEREFGLALITTAPTVEYRVILTSGTTVMIDNPV
                     KMPDPSQIERIEEPFVDATIITPSEYIGNIMALVSECRGIHKRMEYIDQTRVQLTYEI
                     PLAEIMFNFYDKLKSCTRGYASFDYELTDYRASDIVKVDILVNEEPVDALSFMVHKDK
                     AYKRGREIIEKLKDIIPRQQFKIPLQAAIGSRIIARENISPLRKNVTAKCYGGDITRK
                     RKLLEKQKEGKKRLKQIGSVEIPQEAFLTILKID"
     gene            29494..29949
                     /locus_tag="PLV81_06545"
     CDS             29494..29949
                     /locus_tag="PLV81_06545"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HOM09795.1"
                     /translation="MKILVLSDSHGNIQKIKNIIESEKSFDYLIHCGDGINDLIHVVI
                     PDGVAVITVCGNIDRARGINGKTALVEHIHGYTFFITHGDLFGAHHDITGVWSEAKRL
                     GCDIACFGHTHRAMNDNVNPYMFNPGAAQSGMYGIIHINNGIKGELKNV"
     gene            complement(29958..32633)
                     /locus_tag="PLV81_06550"
     CDS             complement(29958..32633)
                     /locus_tag="PLV81_06550"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOM09796.1"
                     /translation="MNKTVYTLEQIVSDFSMASKDLAAILTNDIQKQIIESPYFGGVV
                     SFPLIAEKETIGSFDIIYPKNHTILDENEFSTLELITRLIANGLRQYRYQNKIIISET
                     KYSSLINAIPMALYVINRDYTIIETNDTFKEWFSIEHPLNKKCYEIIALRDKPCNDCI
                     ALRVMNLGTSFIREQKVPYFDDSRFFKYIASPIIAPSGIVTSVVNILEDITTRIVAER
                     KIEKFSLALAEEVDKQTKELKEKQKKLELVTNTLYLIKQANTIKESIHHITKSLHSLG
                     AKLSIVTLLSSDVNTLQIVDVYPDNLLHTLQIVARENILNMKLNFNDYATIPFFAVIK
                     SGKDIIIDTKNELKELIEKTFPGIDDNNKNLLFSQLQDEIIAIYPIGTKHRIEGSIAI
                     ALDKSSNEDNKDFINILLNAAAVEISRKRNQEELVKSELKYRNLVENTQDIIILCTAE
                     GEIVYGNSVFYKLLDYPESHKPSFFDFIDESEKQLLQTIMKHSLSSSTNPQPFEIQLY
                     NRHGKYSWYELAMSLVPLDHTIGIQIVARDIERKKRMEAHIQNLTEFQEKILQNEMIG
                     IITADLNGVITSWNKGASTILGFQPFEILNTPLNQLVNAIDNKQILLKSSTKNPVITQ
                     PYAEITMLKKSGEKIITLCMSSLLHDDHDIPFAHLYFFIDISEKKRLEAESLELNQRL
                     QHAQIVTIVSLAKLAEYRNKETGMHLERIMRYVEILAYELSQHPEYQNYITQEYIRDL
                     VNSCLLHDIGKVGVPDHILLKPGKLTEYEFEIMKQHTIIGAETIAEAERKVAGRSYLN
                     LGKEIACYHHERWDGSGYPKGLKGNEIPLSARIVAIADVYDALVSERPYKRAYPHEKA
                     VKIIADKAGIYFDPVVVTAFLKNHNKFFEIKESLL"
BASE COUNT        10993 a         5135 c         6239 g        10958 t
ORIGIN      
        1 ttaaggtggt gaagagaaat gcaggtagtc gtttctatgt tcatgaaaac ctccacagta
       61 gtgatagggt aacagtaaaa tgaaagaaag aaaatgcaag aaaaaattat atatgatata
      121 gtacatagca cagcagatgt gaagttcagc agtaactatc gcaacaagaa aaacagaaat
      181 tttacttcca attgatgccc atatgaagta agtgctatcc tgtggttaat ttgacggcta
      241 cattttaaca atattctgga ttctgtagat gctataatag ttttttttct tctaattttt
      301 tctgaatgga ttgtagttgc ttgtagttat taataatttt gatcatatag cgattatttg
      361 gatcttgtga aaggtcaatt tctaatggtt tttcatagaa acgtgccagt aactgttcac
      421 ctgttctctg gaatttgcaa aatattatca ctttaggttt cagcatgtca ttgataccaa
      481 agtccttaat aatagcaagc tcctgagtgt tcagttcgca aatatcatta tgtgtgtaga
      541 atgcctgtgt gttgtttaat ataggaccta acttattgat gaaaatgtta attaattgat
      601 aatcaaactg ctgattaatt gcatttaata ttgcctttaa tgcattactt ggttcaatag
      661 catccctgaa tgggcgtttt gatgttaatg catcatatat atcgcataat ccaattattt
      721 tgggataaat gggtaaattt tcatatggta atccgtaata cccattatta ttgtaccgtt
      781 catgatgaaa taataccgat tgcaatacta tggggtgtac atctttaatt tttttaagtg
      841 tttcataacc taactgggga tggcgcttca ttttctgaat atcggttatg ttatattttc
      901 cctctttttc taaaaggttt ttatctaatt gcatctgacc tatatcatgt aaatatcctc
      961 ccagagcgat atttttaatt tcatctttat tgaaacttcc aatcattcgt gccataactg
     1021 cacttaaaat acctacgttc actgaatggt tatagatata ttcttcatag cttttcaaat
     1081 cttttagaag gtttaacgcc aatatttcgg ttgaagtaag gtcgttgaca atatctatta
     1141 taaccttttc agcttcgtgg attgctactt tgctgatttt atggctttga gctatttcat
     1201 gcattatttc ttttgattta ttccgggcaa tttgcatcct gaaagtggga ataacagcca
     1261 tctgtccatt gtcattgtaa tacaggatac tgccgtacct tgctttaata gcatttattt
     1321 tttcggcagt taaagcaact cgaggaggta aaactacatc accatcttca gatataagtg
     1381 gataaatgaa agtactattt ggtctaagat aatttacatc aacttttatt ctttttccaa
     1441 gcatgtataa tagttgcacg ctaatattaa attcaatata aaaaaatcaa tgatataaat
     1501 caattatatt tttttcttca aaattaaaaa ttttattagg tattatttta agactgaata
     1561 gaatacagcc cacctgatta agaccttgtg gagcctacta tttcattttt atacatacaa
     1621 ataagttaag gtataaaaag ttataatcta taaatacgta cgtttttcaa caatactcac
     1681 aaatggtaaa aggaatagct aaaattgaaa attacgtaaa tacagtaaag tttttaattg
     1741 tttgcagtag tatttgtgct gcattaagag catcttcttt tgttaatcca caatcattat
     1801 ataatgaatc agccataaat ttacgtatag ccatatatac atcttctgtt gggtaaaaaa
     1861 ggaatgcaaa attttcaccc tgtgttttta agatagtaaa tgatgtatat aacatcacgg
     1921 gttctttagg tatcattatt ttttcctgct tttgcccggt tattattgtc aggtcatcaa
     1981 aacggtatgg tattgatttg cctgcttctc tgtgaactgg ttcttcatat ctacgggcat
     2041 atgttggagg ttctaccagt aaatgtgttc tgcttccatc agttttaaat gtaaggcctt
     2101 ctgctgtttg ataaatatct ttaacatttt tatatgcggt tacttcataa attgaatata
     2161 tggaatcagc gtcaaaaaat gaacaaatta taaatccacc gtttgatggt attgtgttgt
     2221 tcatgtaggc cttaaaaaga gctgtgcctt tagccatata ctacctctca ttatcactca
     2281 aaataataat tgatgtcatt aatgacaatc ttaaactact ttagtgtagt cgagaggaaa
     2341 ataaaatgca aatctttttt ttacttttta aacgcctgag caatgtatta cttcaaatta
     2401 tatttatgaa cataataact ctcaatgtat acaatgtaca tgtttaattt caggagtgtt
     2461 ggttagtatt aataaaatga ttatatagac atttagtaag gagatttttt ctttttatta
     2521 tatataacaa gagatagaac agtaacaagg aaaattgaaa atattattaa cttatattta
     2581 ttcaaataag gaaatatgat ttcgtaattg gaacccaggg tatatccaat ggtaaacaga
     2641 atggttgaag ttatggatag tgcaagaaaa tcaaccagca tgaatttgta taatttcatt
     2701 ttagccaggc ctgctgtcaa aaagattaca tttcttacac caaatggaat aaaccgccca
     2761 aaaaatagtg tttttgcccc atgtgattga tagtaatatt caattttttg aattttatcc
     2821 agaggataaa atttagctaa ctttttatgt tgcaagataa aattcatacc aaatctgcct
     2881 aaacaatatg atattatatc acttgagtaa gcaccaagaa cacagccagc cgcaatttta
     2941 aatgtatgtt ctggtataat tgttgctgca atagaagcag atataataaa tattatatct
     3001 tcagatattg gaaaattaaa tcccgctaac agtagaagta taaatgcaat gtagtgtaca
     3061 tggggcgcaa aatcaaaaag aagctgggag atagttgcca tagatgaccc aaacaatagt
     3121 aaatttattc aataataaat ggctcatatt ttatagctac agtgcctttt tttacccaaa
     3181 tgcggatttg taaagcattt ttcccatcca gagatgagat tgttcctaat tcttcaacca
     3241 ggtcaatagt atcaattgaa aattcataaa atattttttt atctgaaaat ggatcggata
     3301 ataaaataat ttttgtttta tcatattgat gctttttagg agttcctaca aacgctacat
     3361 ggttttcaaa aaaatttata tttcggtatt tatcaattgc agttttatcg ttttctttga
     3421 gtaaatttaa taatttaatc tgttcttcca taactatctg ccataccttt tatatttttt
     3481 taattccatt aataataatt tcaatggtct cctgtactat tatcccctgt aatggtttat
     3541 agctatcaat aataagtaga ttaatcattg tcgtgataat tccgcaacat acttttgata
     3601 atattggtat atgtgcatta cgaataaaac catgtgatat gccaatagtt aaaaactttt
     3661 ccaattcctg ttgtataata tgttgcaatt tttcagggaa ataggcaaat tttgaatcat
     3721 atgcatgtag taataaaaaa cctaattcac tagtaaattt aagcgcaaat tcacttaatc
     3781 tcaaaatttc ctgtttaaga attataaaat attctgcttg tgtaatatgt tccggtaaag
     3841 gtgataaaat aaaaattgaa tcaattgaat caactatagg ctggtaaatc tctgttaaaa
     3901 gcatttcttt atctttgaaa taaacataaa atgtaccggt tgctaaatta caataatcca
     3961 taatatcagt tattttagtt tttgaataac ccttttcatt aaagagtttt tcagcagcat
     4021 agaggatttt tgttttagtt tcaggtttaa caatttgagg catcgtttaa ccttttttat
     4081 caaatatact gtaacaattg taaaatagta tgtatgtata atcaacaaaa taaaatcttg
     4141 acaatattta aattttctta catgttaaat taaagaagta caaagaagta ccaggtataa
     4201 aaaaataaag aatatgaaga aactaacagg taaaatacat cctcagcatt taccagcatt
     4261 tattatagct attgctatac tattgagttt tattatcatt caaaaaattt atttcactat
     4321 actgctaaat caaaaaataa cacctcaagt aatagaggaa atatttgaaa ataacttcaa
     4381 aaaagcaatc cagtttgaca gtagtagtgt tacactgggg ggaaatatag ttctaaagaa
     4441 tgtcaagata gcaccaacta ctgattttaa tgataattat aatttgatat catgtaaaaa
     4501 agccataatt gatttgaact atattaaggc aataaccggc agtatggtta taagaggggt
     4561 aacctttaca gatgcttcta tgactattgt aaaaaattat ggtaaaagct atctggaaac
     4621 ttttaaagga atattttcag gtatagtgaa cggtaatatt ataaatcttg atgatttcta
     4681 tatggaatta gatggtaatt tattgtataa tgaaaattta agtgcagata aattaaactt
     4741 aagcattcaa gatgtatcac ttgctatttc aatgaaaaat aaacatttat catatactat
     4801 taaaggtgaa gttttaccaa ctgatagtac cctcgattca ggcagggtac atatatccgg
     4861 gattattgat tataccgatg ctatgggtta taaagcatca catcatagta tatatgctaa
     4921 aaagttggat atgcatatag taaattattt tttaaaagaa tattctgaat acccgataac
     4981 tgtgagagga tatttttata ctgattgcac tatcgatcat gattccgcgt attcgtttaa
     5041 aggtacagtt gaatttgata attgtacaat tgaaaacatg aaaaacattc cacattttga
     5101 atacatttcg aaagataata taagtattga tgcactgatg gaatgtaatg aagatttatc
     5161 tgaaattaat gtatcttctt ttacatttaa tgataaagag atttcaattg atgctacctt
     5221 aaagtatagt aaagataaat tactacagtt ttatttttct acaaatcaga tagatctgga
     5281 tgattgtgat tatattcagt tagtaccagg gctgtattat gatggttcat taaaaataat
     5341 gacacaatgt gattttgata ttgctaacga tacgatgaag tcatttaaaa tattatgtga
     5401 tgctcatgat gtatctgtat ttaagaaagt taataatgca actacctcat atttaaaaag
     5461 tacaaggtta aaagttgagg gtgatgttaa atctgtaatt cttgatttct ttgccaaaca
     5521 caaaaaatct gatatactga tacagtcaaa aattaatatt gataaatggg caccgttttc
     5581 ttccactact gacataacca ttacttcaaa acagcttcat tcggatgtgc tggtgtatct
     5641 tgtggctgat ggcatatcat cgttatatac tggtgctatg gaggatatgg gtttaggtta
     5701 taatgagata ttttttagag ataagcctct ggggaaatat ttaatcaata atattataaa
     5761 gctaaaatgt aatattggga agttactatt tgataacaat gcacatttga agaatataaa
     5821 aattgatata ctatccaata aaggtacaat atcaacacca tcattcaact gtgaaggtta
     5881 ttcgggaata tattctttca atataaatgc attttgcaac tgtgactatc ccaatcttac
     5941 tattactgct ggtgtatcca actttgatta tggcagatat ttgcaggatt gtggtcaaaa
     6001 ggatgcaaca ggtgttatga atgtcagttt aaattattcc ttgtcaggtt atcgtttaag
     6061 tcatttgctg cagaatggca atggtttatt acaggtaact atcgccaata ctatatttga
     6121 taaaacactg ttacagaaaa agattggtga aatggcaaaa atctgtaata tatcatttgc
     6181 tgatggggtg tggcgctgca acaggcttga tttaaccatt aaccatatag ctgacagatg
     6241 gattttacaa aatatgttta tagatacaga tatggtacaa cttggtggtt atggaaacta
     6301 tacaattgat aaaggcttgt cgttgccatg ttatgcaaca gtatttaccc gagaggggcc
     6361 ggtcatcaaa ggatctcaaa ggattaattt tgttataacg ggtacccttg ataatcctgt
     6421 gattgcgacc ggtactccct gtggcaaaaa agaaatatca ctctttgatg tcaattaatt
     6481 cataaatttt taccggtaat tgcttacctt taacccttac aaggtcaagt tctcgcgcta
     6541 tgacatgatc tttcacatgt tcatatgtga attcactgat tataatgcct gtgccatact
     6601 gtttattggt accttcaaga cgtgcaccta agtttacatt atcacccatt aaagtataat
     6661 ccattcgtga ggcagaaccc atattgccaa ctaccatttc accggtattg attccaatac
     6721 caatatgcag ggcaggtttg ttcatctgta cccatcgctt attcagttgt tctaaaacct
     6781 gcatcatttc aactgcacat ttacatgcta acaacgcatg attatcctgc gggataggcg
     6841 ctccccagaa agccatgatt tcatcgccaa cgtatttatc aagtgtaccg tcatatttaa
     6901 aaactaactc agtcattgcc tgcaggtatt cgttaagatg ttcaaccagt tcttctggtg
     6961 tcattttttc tgaaatggta gtaaaacttc gtatatcaga gaataatacg gtgagaatct
     7021 ttttgtcacc acccaatttt aatttatcag gatgtttcaa aagctcatca acaacagttt
     7081 tggaaacaaa tttggaaaaa gtttgacgta tatatttttt ctcctgttgt tctgttaaca
     7141 ctctatatgc aataataatt gagaatgttg ttccaacctg aataataggt gttgcaaata
     7201 tagtaattat attgaatata tcaaaaagca ggtaggaacc tattatgtag aataatacaa
     7261 gcccaagagt aaaggcaact gacacaacta tagaaacctg tgtaaggata aaccctaaaa
     7321 gcagggaaat gaaaagcaaa atcagtaaat tctgccatgg tgtaaattta agtatgaaat
     7381 cctgattcag gattgtattt aatgcatttg catgatgttc aataccaaaa aggtcgccat
     7441 aaggagattt atgaatatct gttgaaatac ctgttgatgc atacgctgct acaagaatga
     7501 ttttatcttt caaagaagtg ttttgtattt ttccatctct gcagaaataa tagtatggat
     7561 agtgtgtaaa actgcctggc ccaccaataa aattaatatc cataaaacct tcatcatcaa
     7621 ttggaatagt tatgctgtta tcaggagttg gttttgccat tttgtccctg gggatgtttg
     7681 ataactttat atagttcccc atggatatgg ttatatcttt ctttgtaata ccatagtaat
     7741 gcatgactat cagaaggtca atggaaggat aatacctgcc tttatatttt attactaagg
     7801 gaacttttcg gttaacatgg tcagcatcgg gacgtatatt tgcaaatccc aacccaattg
     7861 cggcctgaga aagtaatggt gtgggaggaa cagcttcctc aacccacggt actgaattgt
     7921 cctgtgggtc aacaggaaaa gaatagatat ctaatatgcg catgcgttct tcaatatcag
     7981 tgtatttaac atcaatttct tcggtttcaa atggatagtc taaaaaaaca cagtttgctt
     8041 ctttgatggc tttggcaaat tcttcttcat aaggtttatg atccagaaac atgatgtcaa
     8101 aaaacattgc tttaggttca cctgatccaa cgtatcgggt aaatttagtg tggtagttcc
     8161 agggaaatgg ccattttatg ccttcgttat caaattcacg gatggtgttt tcatcaattc
     8221 caagaataat aatgtcatca cgggagcggg gatttttacg cgacatcctg accccttcct
     8281 gcagtttaac cgctttttca gaagggtcac gtaagtaaaa cctgaaattg actgaaccgt
     8341 attcaaggcc atctaaaaga ttggtttgtg tatataaaaa ataaagaata ataaatacaa
     8401 ctactgataa aaccaaaccc agaaattgat tcttattaat ttgcatagtg gataccctta
     8461 tgatttataa gataatagtt tgatttttga ttaaaggaat ctgtaaaatg caatacaata
     8521 attatgtgat aattgcaagt tttttattaa attcttgaca aaataattaa tgcaaaaaat
     8581 caatttgtat cttttcaatt aatttttcgt gatatactgc atattataaa actgggaatt
     8641 atgtaaagag gtgttgtttt gataatcaag gagtatattg cacaaactgt cggtcaggct
     8701 ttaacaaaac tattgcagga aaaaaatatt tcaacagagc tatcgccagc aataaaagtt
     8761 gagtatccca aagaggaaaa atttggtgat tatgccactc ccgttgccat ggagtgtgca
     8821 aaaattctaa ggatgtcccc aattcagatt ggcgaagaaa ttaaacagca tattaatgct
     8881 gatgtttttg atcgtataga tgtggttaag cctggcttta tcaatatatt tctatcactg
     8941 ccatttcttt tacaaagatt agaggagata gttactgtca aagatgaata tggtaaaaat
     9001 aaaaaatcca atccccgcaa ggtcaacatt gagtttgtgt cggcaaatcc tactggtcca
     9061 ttaaatgtgg tttctgcacg tgcagccgca gtgggagata cccttgcaaa tttgcttgct
     9121 gcaagtggtg atactgttca ccgtgaattt tatgtcaatg attatggcaa tcaggttttg
     9181 tggttaggga aatcagtgtg gatacgctac aggcaaatgt taggagaaga tgtcccattt
     9241 cctgaagaag gctatcatgg tgaatatgta aaggatattg ctaaatacat acacgaaaac
     9301 ttttcacagg aactatcgca cataaacaat gaggaagaga tcatcaattt ttgtgcacaa
     9361 aaagctattg aatataatgt tgcatcgcaa aaagaggatc tggataaatt taatgttagt
     9421 tttgatacat ggtttcatga aagcaccctg catcacagtg gcaaagtatt ggatgttttt
     9481 aaatatttag aatcacttgg cgttatacga gatgaagatg gcaaaaaggt ttttgtttcc
     9541 acacagtttg gtgatgacaa ggatagggtg gttgtacgag atgatggtag gccaacatat
     9601 ttaatggctg atattgcata ccacaggaca aagattgaac gtgggtatga tttaatcatt
     9661 gatatatggg ggcctgatca tcatgggtat attgcccgcc tggttggtgc aatgaaagca
     9721 atgggttatg atgaaaaacg attcaggatt ctgatttccc agcaggtaaa ccttcttatg
     9781 aatggtgaag cggtgaaaat gtctaaacgg ttggggacat tttccaccat gcgtgatctc
     9841 attgaagaga ttgggactga tgttgcgcga tatttctttg tcatgcgttc aatggacagc
     9901 catttagatt ttgatattga cttggcaaaa cgccagagca gtgaaaatcc tgtattttat
     9961 ttgcagtatg ctcatgcccg aatctgttca atattcagag aggctcagaa cagaggtatt
    10021 gagtacaaaa aaattattac caatactgag tattttaata atcaggaaac aattgcttta
    10081 ttaaaactta tggcacggtt cccggaagaa gtatatgatg ctgcatcaag ctttgaacca
    10141 caccgtatta ctgtttactg tatgcgctta gcacaggcat accataaatt ttatactgag
    10201 cacagggtat taaccgataa tgaagaccgt acccagagct atctcacatt atgtgatggg
    10261 gttcgtataa ttttgaaaaa tgctcttgca ttgcttggtg tgagtgcacc tgaaaggatg
    10321 taaaaattgc attaaaaaaa ataaattatt gattttttat agctgcaatt gttgtattac
    10381 agtaaatcat aaagtaacat ttaaagatat tccatgaaag cttcgctaat agctacaggt
    10441 agtgaactgg tttgtggccg tgttcatgaa agcaataatt tttatataag tcagcacttg
    10501 ctggcaattg gattgccagt gatgatgcac tatgtagttg gtgatgatag cgaaaccttg
    10561 cgtctggcaa ttgagcatgc attgcatagt gcagatatta ttataatttc tggtgggtta
    10621 gggcctaccg ttgatgatat tacatttgaa actctggaaa aaatgtttgc actaaaacct
    10681 gtgattcacg agccttcaaa agaacgcatg gaatatttct ttaatgctat tgggaaatca
    10741 gttttgcctt ctgattttaa gatggtgact gttcctgaaa atgcagttgt ttttgttaat
    10801 gatgctggac ttgcaccggg ttttgctatt actgttaaag aaaaagatat tatagtattg
    10861 ccaggggttc cgcgtgaatt acattccatg attggtaagg ttattgatta tctggaaagt
    10921 cattatcaac tgaaaaaaaa gaactctgta acattcaagg ttgttatgat gcgtgaagct
    10981 gaagttgatt cgggtgttaa acagatagtt aattcaataa agggtgttga tgtttccatt
    11041 acgtatgaag ccgggatgac tactgtaatg ctcactgcta acgatgaaag tatattgcca
    11101 gttgatagta tagttgataa agcaaaagag ctttttggct caaaaatggt atatggatat
    11161 aattcattag aagagaaagt tgttaaggta ttcgccagtc acggtaaaac aatagccatt
    11221 gcagagtcat gtacaggggg gcttattgcg aaaagaatta cagatatacc gggttcttcc
    11281 aatgtgttat tgggtggtat agtggcatac agcaaccagt caaagatatc gatgttaggg
    11341 gttaatcctg atactttgca aacatatggt gcggtaagta cggaaactgc attggaaatg
    11401 gcagcaggtg ctaaagaaaa atttaatgcc gattatggaa tttcaacaac aggtatagcc
    11461 ggtcctgatg gtgggaccaa agataaaccg gtaggattgg tatgttttgg cattgcaggc
    11521 agtaatactt ttactacaca gatacaattt gctggtaatc gtgaacggat acgtacaatg
    11581 gcaactaatt acatattaac cagactatta acgtgtgcag gacttgaatg aaaagagtat
    11641 ttatactatt tactatatta ctttttttca atgctgcttc gtatgcaata gaaagctcac
    11701 agctttttga gcagggtatg gaagcattca ggatggggaa ttatggttcc tcagaactta
    11761 tattcagaaa agtaattgat gctaatgatt cttttaagga cagagcatgg tattatctgg
    11821 ccttatcaat attttaccaa aaacaatacc gttcggctat ttttgagtta aacagatttt
    11881 tacttttatg tacaacgcct gatctttgta gtgaagcgcg gtactggatt gcagaatcat
    11941 attattacct gaatgattat atcaaggcta ttgaagaata taacaggttc atttctcaga
    12001 gcaaaaataa acagctgata ataaattctt atgaccggat aggtgaaata tatttcaatc
    12061 agaaacgcta tgatgaagca attattgaat ggaaaaaagc attaaatata agcagtaaaa
    12121 aatatgaaga cagcaataga gttataaaaa ttggagaagc gttgttctta agcgaacgtt
    12181 ataatgaagc tctggactta ttagagcctt tgatttatgc tacaggtgat aataccattc
    12241 aatcaaaagc caggctactg gttggaagga tatatcagct tactggcaaa cactggcagg
    12301 caataaaata tttcaatgaa atacctgagt caatgttacg agtgccatct tttataagtg
    12361 tacagtattt taaagcccta tcatatatag ccctgggtga tacatataat gcccgattaa
    12421 acctgaaatc gtatattctt atttctgctg atactgtaca gtatcattat aatgcaaaat
    12481 atgagttggg gaaaatacta ttaaacacca gagattctga tgaagggatg tccttgttgg
    12541 aggatgttta tacaaatagt aatgatattg acttaagaag taatgcttct tttgagctgg
    12601 caaaagtgta tcttaaaaat aacaataatg agaaagcaat cccatattta gaaaatgctg
    12661 tttctatcaa tgatgcggaa agtcagaaat acgtcatgat gaccttaacc agattatata
    12721 taactactgc acggtatgat gatgcaaaac gtcttctgga ttttatgctg gaaaaatatc
    12781 agtatgaaga aaacagagat gaaatgcttt ttttaattgc acaggtttat gtaatgcagg
    12841 gaaacattac aaaagcactt gaagtgtttg aagatataaa agaaataaat ccattttcgc
    12901 agtacctggt agagcttgat tatgtaaaag caatagatgc gtttaaaaaa gagcaataca
    12961 atgatacaat agactatggt aataggtatt tgaagaatca aaaaattgaa aacagatatg
    13021 aggttttaat tttattagct gaatcatata taaaaataaa taacacaaaa aaaaccattg
    13081 aagtaataga ccagttaata actcgtttcc caaaaaaggc aggattagaa gaaatactat
    13141 atgaattgtg tatagtatta aaagaacagg ggattaacgt tgaacggtat tattcattta
    13201 ttattaagaa ttacacttca tcaattacag caggaaagat atttatagaa aaaggagatg
    13261 aggcgtacaa aaagaaaaac tatcaggttg cagtatattt ttattctcaa tatttagcaa
    13321 caaagggaag accacgtgaa cgttcagtac ttttatttta tatactatca ctctatgaac
    13381 tgaagcaata cacgacaatt ataaaaacat tgcaggatag tgattactta actgtagatg
    13441 atctgacaac aaaattgctg ataaagatgc ggggaaaaag tttttatgca cttaaagatt
    13501 atcctgctgc attatctgat ttcatgaaaa tccccttcca ggattataac aaagaagatt
    13561 ttgtaataat tgctgataca gcactaaaag ccagtcagcc agaaattgca attgatattc
    13621 ttccttatgt tgaagatgat gaatattttg ctgttatttc gcattcaatc gctctttatt
    13681 ataggattaa tggcaacaat gatctggcat ttgattattt tacaaaagcc ctactcagga
    13741 aaccagacag cagtgtctct gatgatacaa agatacgttt ggcacagatt tatattgaac
    13801 gcaaagattt tgaaaaagct ctggatctgg ccaaatccgt tcaaaagaaa gaactgaaga
    13861 atcttaagaa tagtataatg gctgtaactt attttaattt gggcaacaat gataaagctt
    13921 taacggttat tttgggtaaa cttaacgata ttttgaaaac tgaatatgca gaagagataa
    13981 ttacgttttt gttgaggcat tactatattc agggtgatat aaaaaattat cagactattt
    14041 attggtatgg cataaaaaga aacaagctaa tatcattatc aaatcattac tatggcatga
    14101 tgatgtataa aaataataat tattcacggg ctttacagtt tttcaatact gcttcacgct
    14161 atgatgatga gtatgcgcat gcttcattat attatgctgg attaatatat ttattaatcc
    14221 agcgcaatta tggtatagct aaaaagtatt ttgaccttta tttttccaaa gctggtagca
    14281 ctgatatcaa tttttataat gcaaaagtat atctatcatt attgttatat gagacaggta
    14341 aacaggatga gtctaaagat attttatatg aaataattaa ctcagattca aatgtacttg
    14401 caaaaattaa agcagatcat ctcattaaac agtatggttt ttaacagata aatataccaa
    14461 ttagaggaat gtatgaaaca atatactttt catgtaattc atggaccaaa tctaaatctt
    14521 ttaggggaaa gagaacctgc aatatatggt acactaacac tggaagaaat taataacagg
    14581 atacgtgaat ttgcaaagag caagaattgt gaagtacgtt gctttcagtc taacagcgaa
    14641 ggtgaaatta tagattatat tcagaaaaat agaaattgcg atggcattat tatcaatgca
    14701 ggtgcatata cgcatacttc aatagctatc cgtgactgct tagtttcatc agggctgccc
    14761 tgtattgaag ttcacctttc caatatacat tctcgagaac gcttcagaag acattcgtac
    14821 attgcacctg tatgtattgg tcagatctgt gggtttgggt attactcata tataatagca
    14881 ctgcaggctt taatacatta tctggaaaat tactcaaagt gattctaatc ctatgaacta
    14941 tcgcacaaga ataaaaaaaa tacaaaagca tttgtcagaa aaaaaacaat tcccatacct
    15001 ggttgcaagc ttgattaata ttaaatattg cacaggattt gaaggaagtt atgggtatct
    15061 tatatttttt gaagatgaga cagtttttat ttctgattct cgctatgaag aatatgcttt
    15121 gatgatatta cctgatactg ttcgttttgt attacagcaa aaggattttt tcacatcatt
    15181 aaaatcatta cttaaagaga aaaaaataaa aaaaatattt cttgaggagc attcaattac
    15241 actatcgcag tataagatgt taaaaaatga atgtaaagga atagcattta attatggcga
    15301 ggatgttatt aatgagttgc gtattatcaa agatgaggat gaaattgaaa ctatcaagaa
    15361 agcagcccag ataacggatg catgttttaa ccatttactt aagattatta aaccaggtgt
    15421 tactgaatgg gacattgcta ttgaaataga atatttttat aaaaaacatg gatgccaggg
    15481 aacttcattt gataccattg ttgcatcagg agcaggatca tcaatgcccc attacaaaac
    15541 gggcactaaa aagattaaag caggcgaacc ggtgcttatt gacatgggat gcattttcaa
    15601 ggggtattgt tctgacataa cgcgtacagt ttttattaaa agtattcctg atagtgtcaa
    15661 cacaatttat tctatagtta aagaggctca aaagagagct gttgatgcag taaaagcagg
    15721 attgaccaca aataaacttg acagtattgc aagggatttt atagctgcta atggatatgg
    15781 tgatttattt ggccattcgc tgggccatgg cgttggactg gatgttcatg aaatacctgc
    15841 tgtaaaacag aatggagcta tggtattaaa aaagggtatg gttataacta ttgaacctgg
    15901 catatatata cccaaaaaag caggcgtaag aattgaagat atggtactgg taacttcttc
    15961 tggacatgag gttttaacaa aaagcacaaa agaactaatt atcttataga attatatttc
    16021 caaggagtat tccacatgat aacaagtaat gacttgcgga cgggcacagt tattaaggtt
    16081 gatggtgacc tgtattcggt agttgagtac cagcatgtaa aattaggtcg tggtggtgcg
    16141 tttgttcaga caaaattaaa atcacttaca aagggtgtca ttgtagataa aaagtttaaa
    16201 ggtggcgaaa aggttgaaga tgttcgggtt gaaaagaaga ctttgcagta tctttatgat
    16261 gagggtgatt ccctagtttt tatggataca gaaagttatg atcagataca tgttgataag
    16321 aaaaaagcat caaatttact caattatata aaagagggcg atagtgttga agcaaccatg
    16381 catgatgagg aaatattatc tatagagccc ccaatatttg tagtattgaa agtaacctat
    16441 gctgaaccag gggtaaaggg tgatactgcc actaatgttt taaaaccagt cactgttgaa
    16501 actggtgcta ctgttcaggt cccaatattc gtcaatgaag gtgatgtaat aaaagttgac
    16561 acacgaacag gtgagtatgt ggaacgggta aaaaaatagt tgaataataa aactataaaa
    16621 agttattact acttttaaga aaattattgt aagtcagatt atggggctgt cccaaaagag
    16681 gtcgttgagt aaattttgcc aacattatat cgtaatactc gctcatttcg atacaatttt
    16741 accataaaat ctggcaaaat tactcaatga gcgttgcgaa atttatattg aaacgacttt
    16801 tgggacaccc ccatagtcac ctgaattaaa atttaaaatt aaaagattta caatgtgaaa
    16861 tctgaaataa taacaaaaga agcttataaa gcgtttaaaa aaggttcata tgtagtagtt
    16921 attcagttat tagaaaaaaa tatcactatt tcaaataacc cctatgatta ttttttattg
    16981 attttgtcgt acttgtttac agataaactc atgcaggctg aggctgcttt acgcaaggca
    17041 ctacggcaat atccagcgta tcttcctctt ctggaaatac aggcatttct tatgttaaaa
    17101 tctgctaaat caaaagatga ggcctgtaca ggatacattg atatattaaa aatttcccca
    17161 ggaaataaac aaataacaaa aattatagca aaaatacagg aatctgctga ttttgaacaa
    17221 tttcaaaaaa aagctaagtt aagggatttt atcaaggtta aagcaccacg aacaatcaag
    17281 acagtttcat tcagagcaag acggcctact atacactaca aaactgtagc tttaatagtg
    17341 atcattattg cgtttcttat tacagggatt atatttttcc cttcacctct tgcaaaactc
    17401 atacaggaaa aaattgaact gttatttttt catccagcac ttcaaacagg tgaagatttg
    17461 tcacagatat cacttgatgg catgcattat gatatcattg ataaaatcca aaaagtgcgc
    17521 acacctgaat tttatgcttc aaatgaagca tgtattcagg atttcaatca ggcaaagtgg
    17581 ctgatcaagg aaggcaaaga aaatgatgcc atgatattgt tgaacaagat tgatgccagc
    17641 aatgcaaatt tcagagttaa agagcgagtt gcatttttaa aagattttat acaccgcaac
    17701 agggagcgta cggtggcaaa attttcttac agggatgtca tccagaaacc atatttatat
    17761 aaaggcataa atgtaaaatg gcagggtaag gtagcaaatt ataaaaaaaa ggatggcata
    17821 aactttcagt tactgattaa ttattcagaa aacagatttg atggcgtagt agaagtattt
    17881 gcgcccttta acgtacaggc attagataat ggggcgatag taactgttga ggctatcatt
    17941 accaatatcg ttggccagaa aatagttata atgcaggcaa caaatgttga aataaaatct
    18001 gaatgacgtt ataataattt atcaatagta ttatcaagag ttacggtatg aaagtaatag
    18061 taaatgaatg cagccattat gacgttgaag ctattaaaac gaaggttatt gcggcattaa
    18121 acaagttgtc gtttgatttt tcaacattgt ataatgcaaa agtcgcacta aaacctaatt
    18181 tattaacatc agcatcacct gaatctgcag ttattactca tccagtattt tttaaggcaa
    18241 tagttcaaat tgtaaaacaa tatggcggga tcccagttct tgttgaatct cctgctgttc
    18301 attcattaca aagagttatg aaaaagacag gatatgatgc gatagtaacc gaagaagatg
    18361 tgctggttgc tgatacatca gattatatag taattcataa tgatgctgcc aaacatttta
    18421 agcggtttga agtgccaaaa attttaacag aatgtgatat actcattaat attccaaagt
    18481 ttaaaaccca tgcactgact catatcacat gtgctgtaaa gaatttgttt ggcaccattc
    18541 atggaatgaa aaaatcgcaa tggcatatta aagcaaaaac taaagatgag tttgcccata
    18601 tgcttttaga tttataccag gcttattgta ctgacactgc agttccaaaa actattgttc
    18661 atattgttga tgcaatcact atgatggaag gtgatggacc gggtccaagt gggaggcctg
    18721 cattccttgg ggtaattggt gtgtcgtata atgcaattgc agttgattat gctctgtcac
    18781 agcttgctgg tttttccata gacaatatcc caactattat cataggtttt aaaagagggc
    18841 tgtgtagcat accacagaac attgatatta tcaaagattc agagatttct ggtcgcataa
    18901 gtattacccc gaaagaagca ggttcaacaa aaattcttgg agttccaata ataaatagaa
    18961 tattaaaaaa cctgcttgtt gcaaaacctg aacctgatcc tgaaaaatgt acgctgtgtt
    19021 atcagtgcaa gcagatatgt ccggtaaagg caatcagcac atcaaaagat ggtaaagtgc
    19081 catggtatga ttattcagtg tgtatacgat gttactgttg tatggaaata tgccccgaat
    19141 cagctatttc attgacaaaa ccattattgc aacgcatact gaagtgatct aattactttg
    19201 ttctagatta tactttattt atactctgga tttaattgcc aatctttgca gtcacaaata
    19261 gttaattgta cggtgggaat ttaatcattt taatgaagat attcacagtt tgaggaatct
    19321 atgaagccaa aggatacaat tcgtaaaatt attgttattg aaatagttgt ttttcttatc
    19381 atcatagtgt tcatctgggc agaagaaatt tttgatttac cacacctgct tttaaatgca
    19441 gaacctactc ccgtgaatta tgaagaaagt cttattgaaa catttatttt tactgcaata
    19501 ttcggttttc tggtatatca taccataaga ttattaataa gattaaggaa tcttgaatca
    19561 tacgtacgaa tatgtgcagg ttgtaataaa atttatgtag aaggcaggtg ggtaacactt
    19621 gaagaatatt ttaataggta tgccaataaa aaaacatcgc atggtttatg cgatgattgc
    19681 aggaaatatt ataaaaagtg attagcggag gagggactcg aaccccccac accacggata
    19741 tgagccgtgt gctctaacca actgagctac tccgccttgg tagcgcgggc aggatttgaa
    19801 cctgcgacct ttgggttatg agcccaacga gctaccagct gctccaccgc gcgatcgttt
    19861 taatgaagtg acaatctatt tataatatac attgtagtca atcatttttc aaattcttta
    19921 ttgattgtct tttgtatttt agcaaaaaaa tcaggatgta agttatttgg aaaaagttat
    19981 ttatatattg tattggttgt atattgttag agtgatagat tgataaacta caaaattttt
    20041 gagaaatatg aaatagtttc aatttaattt attgattttt gaatgtatga atttctatta
    20101 tcctcagcac tttcagcatt gaggaaatgc tgatgtatta ttttacacgt aaaatttttt
    20161 aaaaatctac tatcgaaaat ttttaaaaat tttttaaatt tataaatatt attgttgaaa
    20221 ttttgtaatg tgatataata ctgtcaatgt tctctttgag cagagggggt gatattattt
    20281 aagggctaaa accagtaagt ggttttagca ttttgattat gtaaaagcgc aaaaaaccca
    20341 aaggaattac aatccccagg gtggcgaatt taagttaacc ttaaaaacca ttcttaatgc
    20401 atacactcac cgtagtgtag gtgattttat aaaatgaggt ggttgcccat ggagggttta
    20461 ggtacacaca ttattgcgga actcttcaac tgcaacgagt ttcacatcaa caatttaaaa
    20521 aaggttgagg aggtgttaac ggccgcagcc gaactctcta aagccactat catccaacca
    20581 ttctttcaca agttttctcc atacggtatt agcggagtaa tcgtcatagc cgagtcgcat
    20641 atcacaatcc atacatggcc ggaatatggg tatgcagcgg ttgacgtctt cacctgcgga
    20701 gatcataatt acagggtagc tatcgattat atcactgaac agctggaagc agagcgatgt
    20761 gttctgtatg aaatgcgaag aggagtgcta aacggtagcc aggttaaaaa tgtgccagta
    20821 attcgggagg tggaaaatgt taactacatt gactaacgac atacttaaaa caattccgac
    20881 catagaccag tttgaaaagc tgggtgcatg gggattatat tgcagcgttg atattcataa
    20941 ttgtgatccg gacatcatcc gcgatgcaga gatgatacga cagtatgcga ttcagttgtg
    21001 cgatctcatt gaaatgaagc gatttgggga aacacaggtt gtcaattttg gtgaggatga
    21061 gcgtgtagct ggttattcaa tggtgcagct cattgaaact tcaatgatat caggtcactt
    21121 cgcaaatctg acaaatactg cttatattga tatattcagc tgcaaatatt atgacccaga
    21181 ggttgctgcc caattctcgc tgagctactt caagggtagc gactatactc ttaatgtcgc
    21241 attaagaaag taatcagtga ttaaggggac ggtagggaat acctgtcccc ttaattttta
    21301 taaacctacc agttatttat caatgttaat gtttaattct ataatattct actgtatgga
    21361 ggtacaatat gcccgttgaa tcaagtgtat ggattgaaga attgtatgaa gatgaacatg
    21421 gaaggaccat gaaacttaag gttactaata gacttgcaac ctatgattca aagtttcaga
    21481 ggattgaaat atatgataca aaagcatttg gaagaatgct tgtacttgat ggcgtattta
    21541 tgaccaccga agttgatgag atgggatatc atgagatgct ggtacatgtt cctatgtgtg
    21601 ttcatccaaa tcccaggcgg gttcttgtta ttggtggagg tgatggtggt actctgcgtg
    21661 aagtgttaaa acacaaagaa gtagaaaaag ctgatatctg tgagatagat ggtgatgtaa
    21721 tacgtctttg ccgtgaatac atgcctgcct tatcatgttc atttgatgat ccccgtgtga
    21781 caatatatac tgaagatggt gcaaagttta ttgcagaaag gaagaaagcg tacgatgtaa
    21841 ttcttgtaga ctcatctgat cccattgggc ctgctgcagt attgttttca gaagaatttt
    21901 ataaaaattt aagtgaatgt ctggatgatg atgggatagc atcaacccag tcagaatcaa
    21961 tattttatca cccaaatgtt attataacgc ttgcacagta taataaaaag tttttcaaag
    22021 tcccttcgta ttattatacc atggtgccta cgtatcccag tggtatgatt ggtttctcgt
    22081 tctgttcaaa gaaataccat ccaataaatg atttgaaaga agaaaaagca aaagcgcttg
    22141 taaagcaact cagatattat aatcctgata ttcacaaggg agcatttgct ttgccacaat
    22201 ttatacgtaa aaaattagag gggattctgt attaatatcc aatgagtgcc attgattgga
    22261 ttattgttgt tttatacagc atttgtgtca ttggcatagg gtacgtaatt gggaaaaaac
    22321 aaaaaaatca ggctgattat tatatagctt cccagaaagc atcagcctgg ttaactgggt
    22381 tatcacttgc agcaaatcag gtaagtgcta taagcctgat tggagtacct gcttttatag
    22441 ctatcaaaca taatggaggg ctaacgtggt tgcagtatga attagccctc ccgttatcaa
    22501 tgatgttcct cattcttttt gttgttcctg ccttcaggtc atttcagggc ttttcaatct
    22561 atgaatggtt ggaaaagagg tttggcaagc atacacgtgt tatattagca ctggtttttt
    22621 tactcagcag gtcattggct acgggcgtag caattgttgc cacgtcatat gtggtaagtg
    22681 tattgattgg tattgatttg tatatttcta ttataagtat ttcattaata tcactactat
    22741 acacgtctat tggaggaatt ttagctgata tttatactga tgttatgcaa cttgtagtac
    22801 tactgttcag tacggtttta ttttgtggaa taattattta tgctattgat ggtcatacct
    22861 cttttactat tgcttatcat cggataaaaa tatttaatta tgcttcattc gggatacagg
    22921 acggtaatac gttttcgttg gcaccaatgt tatgcggcgg tttcttttta tatatatcat
    22981 attatgggtt tgatcagagc caggcgcagc gttttctttc atgtgaaaac actgatgctg
    23041 cacaaaaggc cattattata aatactatag ttcgatttgt cattgttatg ttgtattctt
    23101 ttttaggaat tttactatta ctgctattat ctgtagataa tacgttacgc tctttgttaa
    23161 ccaatcagca gcctgacatg ttgataccca catttattaa acactatatt cctgctgggc
    23221 taaaaggtgt agtgatttca ggaattttag ctgcatcaat gtcaagcatg gattcagcaa
    23281 taaattcctt aagcgcaatt acctataatg atgcactgaa aatagcctat gaaaaagtag
    23341 aatccataca tgataaaaca aaactattgt tatcacgctt attaactgta ttctggggtg
    23401 tgattacaac attatttgcc attatttttg ctggaagcag tgaaactgtt gtggaactgg
    23461 taaataaaat tgggtcactg ttatatgggc caatctgtgc agtctttatt attgggatgt
    23521 taaccagaaa aaagattcac cagcttgcag ttacaggagg gtttgcaaca ggcattctgg
    23581 ttaatatata tatttggttg tttttgccat cagtatcatg gatgtggtgg aatgcaacag
    23641 gtttttttgc tgcagtactt ttacctttta tattctcatt ctttactgat tcagaatata
    23701 tcagtgatag cggctatact ggcaaaactt tacccaaaaa tacatggttt aaggcgatag
    23761 ttcttggcat atggtttatg gcaataattg gcttttttat gataattgaa aaagtgtttg
    23821 aataattaaa gcttatagta agcatagatt tattgtatca tcaataaaag catcatcaca
    23881 cattataaac tcacagtgtt cacgataagc attgccttgt cttcctgatc aggttccacc
    23941 ctgtagagta tgccatgggg tgatgcaatg aggcttcgtg cttgcaaaaa atttttataa
    24001 tcggatttaa cgccgcatac tttaaccaga attgcattgg ctaattgtgc acctctaaca
    24061 aaaatatcct gatcgcgttt aaatttttct tcaatagttt cattccgtat aggtgaaaat
    24121 gttggtataa agataatttg tgcacctaat tcttttagtg ctataaaatg ttcatccttg
    24181 aaaacatcag cacagatcaa aacactgaaa cgtatgccat acgcagtgaa aatgacatgg
    24241 ctgtttccag gtgtaatctt gccttcttca gcaaaaaaaa gattctgttt gcggtaaaaa
    24301 cctaacatcg ttccattatg gaaaacaaaa gtagtattgt gcaaataatt gccattaagt
    24361 tcgggcatgg taccaccaat gacaacagta tttaattctt ttgatagtac ctgcatgcgt
    24421 tttaactgca aagactggtt atgctgtgtt tgtacatgat tgcctagttt tttattcaca
    24481 aaaaaatatt ccgggaaaca tagaaagtga ggttttatgt tacggagttt gttgactgta
    24541 taatgagtta aatacgttcc cagtgggtac tgatacatca ctattgtata atccataata
    24601 aagccttttt gatggaatag acatttgtac taataatttt aacagtaact atcgcataaa
    24661 tagtatatca ttattttttt atttaaaaaa tttatgcgat agttcatggc aaaaatcttg
    24721 taattataat gattaaaatc ttgacttgcg atatgatgca ctgtactata agaaaaaact
    24781 tttcagaatg tgaggggata tgagcacaat acagacgttc agagtttatc catatttgcc
    24841 tgataacctt aagccattga aaacaattgc atataatatg tggtgggtat ggaactttga
    24901 agcaattgaa ttattccgcc gccttaatgt agaattgtgg cgcgagtcaa atcataatcc
    24961 cgttgctcta ttaggtatgc tttcacaatc tgaattagat gaagcggctg attcagaaag
    25021 ttttatggct catttaaagc gtgtttcaga agaattggaa tggcacctga caaaaaaatc
    25081 atggtttgaa gaagcgtatc caacatataa agacatgcag attgcctatt tcagtgcaga
    25141 gtttggtatt catgaatgcc tgcccattta ttctggtggt ctgggtgttc tggcaggaga
    25201 tcatataaag tcagcaagtg aattggggtt gccatttttt gcagttggcc ttttatacag
    25261 gttgggatat ttccatcagt atttaaatat tgatggctgg cagcaggaac gttatccaga
    25321 aactgatttt cataaccttc ctttgcagct ggttatgaag gatgataaac cattaatcat
    25381 tgatgttgaa ttccctggta gaaatgtata tgctcaggtg tgggaatgca tcattgggcg
    25441 catacgattg tacctgcttg acaccaatat ttctgaaaac agtattgagg atcgtaccat
    25501 tactgatcaa ctctatggtg gtgatgttga aatgcggata aaacaggaaa tgcttcttgg
    25561 gattggcggg tatagaatgc tcaaagctct gggtaagaat atatcagtgt accatatgaa
    25621 tgaagggcat tcggcattcc tgagtataga acgcatctat gatgcaatgg ttacccacaa
    25681 aatgagtttt aacgaagcgc tggagttcgt tactgctggt actgttttta ctacacatac
    25741 acccgtacct gcaggcaatg acagattttc gcctgagttg attgataagt atttgtctca
    25801 ctattaccag aagttgggta tatcgcgtga agagttttta ggtttgggaa gggaaaaccc
    25861 gcatgataaa ttagaaacat tctgtatgac ggtgctggca ataaaaactg ctgcacattg
    25921 taatggtgta tcaaagttac atggagcagt ttcacgaaaa atgtggagga atatctggcc
    25981 gggattaccc gtgaacgaag tccccatcca tcatgtaact aatggcgtgc agatattgtc
    26041 atggacatca gatgaaatga tgcgtttgct caaccgttat ttaggaccgc ggtggattga
    26101 taatcccgtt gataaaacta tatggaaaaa tattgataca atccctgatt ctgaattatg
    26161 gcgttgccgt gagcgcttac gggagagggt agttgcattt gcacgaaaaa aattgcgtga
    26221 acaacttatt gcccgtggtg ctcctcgcgc tgaagttgaa aaagcagatt cagttcttga
    26281 ttcggaagct cttaccatag gttttgcacg gcgttttgca acatacaaga gagcaacttt
    26341 aatattaaaa gatatgcaac gactggaacg catactcctt gataaagaaa ggcctgttca
    26401 gattatcttt gccgggaaag ctcatccgcg cgataatatg ggcaaggaat tgattaaacg
    26461 ggttatcaat ttaggccgag atgaccgatt cagaagcaaa atagtgttcc tggaagatta
    26521 tgatattaac attgcacggt atcttgttca gggagtggat gtgtggttaa acacacctat
    26581 acgtcctcag gaagcatcag gtaccagcgg catgaaggtt gtccccaatg gtgcactcaa
    26641 tataagcgta cttgatggtt ggtggccaga agcattcaat gggaataatg gctgggcaat
    26701 tggcaagggt gaggaatatc aggatcccga atatcaggat gaagtagaaa gcctgtcatt
    26761 gtacaacata cttgaacagg aagttattcc acaattttat gcaagaggtg cagatggaat
    26821 accacgtgag tgggttcgaa aaattaaaga atccatgaag acaattttac cccagtttaa
    26881 tactaacagg atggtgaaag aatatactga aatgtattac cttactgcct atgatagtta
    26941 cgtcaagttc agtaaagata acttcaaagc ttcacgtgaa cttgaaaagt ggaaacttaa
    27001 tatacggcag aaatggcagc aggttactgt tgattctatt tctcacgaaa gcaaaaaaga
    27061 aataatagtt ggttctcaat tgaaagcaaa agctgttatc aacttaggtg aattaaaacc
    27121 agctgatgtg caggttgaat tatattttgg aaatttaaat caaaaaggtg atatcgaaga
    27181 aggtgctgca ttgcctatgc tcatggttga agatctggga aatggtaaat atacatacga
    27241 agggcgcatg ctttgcctta aatcaggtca gtttggattc actgtgaggg ttatcccatt
    27301 acatgctgac atgccacgta aatatgaatc gcggatgatt acctgggcat aatactgttt
    27361 aatcctgctg ccattagcag tgggattatg tagtttttaa tcgatcttta aaattgtaag
    27421 gaatgcttcc tgtggaattt ccacagaacc aatctgcttc aggcgctttt tcccttcctt
    27481 ctgcttttcc agtagcttac gtttacgggt aatatctccg ccgtagcatt ttgcggttac
    27541 atttttacgc agtggggata tattttcacg ggcaatgatg cgagagccta tcgccgcctg
    27601 aagtggaatt ttgaattgct ggcgcggaat aatatctttt agtttttcaa taatttcacg
    27661 tccacgttta tatgccttat ccttatgcac cataaatgat agggcatcaa ccggttcttc
    27721 attgaccaga atatctactt tgacaatatc ggaagccctg taatcggtga gttcataatc
    27781 aaatgatgca tagccacggg tacatgattt aagtttatcg taaaagttaa acattatttc
    27841 agctaatggt atttcatagg tcaactgtac gcgagtttga tcaatgtatt ccattctttt
    27901 gtggatacca cggcattcac tgaccagcgc cattatattg cctatgtatt ctgacggcgt
    27961 tataatggtt gcatctacaa atggttcttc aatgcgttca atctgagaag ggtcaggcat
    28021 ctttacaggg ttatcaatca tgacggttgt tccactggtc aatattaccc tgtattcaac
    28081 cgtaggtgcg gtggtaataa gcgccagccc aaattcacgc tccagccttt cctggacaat
    28141 ttccatatgt aaaaggccta aataaccaca cctgaatcca aagccaagtg catacgagtt
    28201 atcgggttca aagaataatg cagcatcatt taattttaat ttgtacagtg cttcttttaa
    28261 atcatcatag tcgtcactga ttgatggata aaggcctgaa aataccatag gctttgcttc
    28321 tctgaatcca agaattggtt cagctacagg attttgtgca gatgttatgg tatctccggt
    28381 tcgggtatcg gtaagttgtt ttatacctgc agtaatatag ccaacctcac cggcagtaag
    28441 ctgcgagcgc ttttctcttt ttaatctgaa atgaccaact tcggttactt catattgttt
    28501 ttgatttgag aaaagcatta tgatatcatc ttcttttacg gtaccatcaa atattcttac
    28561 ttttgtaaca acgccaacat actgatcaaa atatgaatcg aaaattaatg ctttaagtgg
    28621 cccatcggga ttaccctgtg gaggaggaat tctttctaca attttttcta aaagatcatc
    28681 tattccagtg ccttcacgtg ctgaaacaca aatagctgtt tcaggatcaa gccccaggct
    28741 ctttttaata ctttctttgg ttcgggggat atcagcattg ggcatatcaa ttttattaat
    28801 aacaggtact atggtaaggt cgttttcaag ggcaagatac atattggcta ttgtttgtgc
    28861 ttcaacaccc tgagtggcat caacaagaag aagtacacct tcacatgctg caagtgcacg
    28921 tgaaacctca taggtaaaat ccacatgtcc gggtgtatct atgagattga atgtataaat
    28981 attcccatct tttgagggat agttaagtgt aataacattt gcttttatgg tgatcccgcg
    29041 ctcacgttca atgtccatat cgtcaagaag ctgatcaaca tgattgcgtg gatcaatggc
    29101 attacaccgc tcaatgatac ggtcagccag tgttgacttt ccatggtcaa tatgggcaat
    29161 gatggagaaa tttcgtatta acgactgttt catatacatg caacctttcg aattcttttt
    29221 ccacccacaa acataaaaat atacctataa tatagcgtgt aaaagtatga aagtggatct
    29281 tttattagca actattttaa aaattattgt acaaataatg aagcacatcc tcctgatacc
    29341 tctggcagca gaagagtgaa tgagcaattt caggtaaaaa attattgttg aaataaagat
    29401 attaatgtaa aagacgtata ttctatagcg ataattatta gactgtgaac gtttaagtga
    29461 taaataaagc aagtattttt tatgggaaga attatgaaaa tcctggtact gtctgattca
    29521 catggaaaca ttcagaaaat aaagaatatc attgaatcag agaaatcatt tgattacctg
    29581 attcattgtg gtgatggtat aaatgatttg atccatgtag ttatacccga tggagtggct
    29641 gtcattacag tatgcggcaa tattgacagg gcacggggta ttaatggaaa gacagctctt
    29701 gttgaacata tacatggata tacatttttt attactcatg gagatttatt tggcgctcat
    29761 catgatatta ctggagtatg gtcagaagca aaacgattgg gatgtgatat tgcatgtttt
    29821 gggcataccc atagggcaat gaatgataat gtaaatcctt atatgtttaa tcccggtgca
    29881 gcgcaaagcg ggatgtatgg aataatacat attaataatg gaataaaagg ggaattgaaa
    29941 aatgtgtaat attgttacta taataaagat tcctttattt caaagaattt gttatgattc
    30001 tttaaaaaag ctgtgacaac cacagggtca aaatatatgc cagctttatc tgcgattatc
    30061 ttaacagctt tttcatgagg gtaagcccgt ttatatggtc tttccgaaac aagtgcatca
    30121 tatacgtctg caatagcaac aatacgtgca gataatggga tttcatttcc ttttaatcct
    30181 tttggatatc ctgaaccatc ccatcgctca tgatgatagc atgcaatttc ctttcctaaa
    30241 ttaagatatg aacgccctgc aacctttcgt tctgcttcag ctatggtttc agcaccaata
    30301 atggtgtgtt gtttcataat ttcgaattca tactcagtaa gttttccggg tttaagcagg
    30361 atatgatcag gaacaccaac tttaccaata tcatgaagca aacatgaatt taccaggtca
    30421 cgaatatatt cctgagtaat gtaattttga tattcgggat gttgcgaaag ctcatatgca
    30481 agtatttcaa catatcgcat aatgcgttct aaatgcattc ccgtttcttt attgcggtat
    30541 tctgccaatt ttgccagaga aacgatagtt actatctgag catgctgtaa ccgttgattg
    30601 agttcaagcg actctgcttc aagtcgtttt ttctcactta tatcgataaa aaagtaaaga
    30661 tgggcaaatg gtatatcatg gtcatcatga agaagtgatg acatgcacag cgttattatt
    30721 ttctcaccag atttttttaa cattgtgatt tcagcataag gctgagtgat tactggattt
    30781 tttgtagaag attttagtaa tatttgtttg ttatcgatag catttactaa ttgattcaga
    30841 ggtgtattca aaatttcaaa tggttgaaat cctaaaattg ttgaagcacc tttattccag
    30901 cttgttatta caccgttcag atcagcggtt ataattccta tcatttcatt ctgtaaaatc
    30961 ttttcctgaa attcagtaag attttgtata tgtgcttcca ttcttttttt tcgctcaata
    31021 tcacgtgcca ctatctgaat gccaatagta tggtcaaggg ggactaaact catggccaat
    31081 tcataccatg aatatttacc atgacgatta tataattgta tttcaaaagg ttgtggattt
    31141 gtactactgc ttaatgaatg tttcattatt gtctgtaata actgtttttc gctttcgtca
    31201 ataaaatcaa aaaaagaagg tttatgagat tcgggataat caagtagttt ataaaagaca
    31261 gaatttccat atacaatttc accttcagct gtacatagaa ttattatatc ctgagtattt
    31321 tctactaagt tacgatattt aagttctgat tttacaagtt cttcctgatt acgcttgcga
    31381 ctgatctcaa ctgctgcagc attaagtaat atattgataa aatccttatt atcttcatta
    31441 gatgatttat caagagctat tgctatactg ccttcaatcc ggtgttttgt tcctatagga
    31501 tagattgcga taatttcatc ctgtaattgt gaaaagagta aatttttatt attgtcatca
    31561 attcctggaa atgttttttc aattaactcc ttcaattcgt ttttagtatc tattattata
    31621 tctttgccac ttttaataac tgcaaaaaat ggtatggtgg cataatcatt aaaattgagt
    31681 ttcatgttta aaatattttc ccgtgcaaca atctgcaatg tgtgcagaag attatcagga
    31741 tagacatcta caatctgtag tgtgtttacg tcgctactga gtaatgtcac aatacttaat
    31801 ttagcaccta atgaatgtaa tgatttagtt atatgatgga tgctttcctt tattgtgttt
    31861 gcctgtttaa tgagatataa agtattggta accaactcta attttttttg cttttctttc
    31921 aattctttgg tttgtttatc aacttcttct gctaatgcta aactgaattt ctcaatttta
    31981 cgttcagcaa ctatacgggt tgtaatgtct tcaagaatgt taactactga agtaactata
    32041 cctgatggag caattatggg tgaggcaata tatttaaaaa aacgtgaatc atcaaaataa
    32101 ggaacttttt gttcgcgaat aaatgaagtt cccagattca tgactctgag tgcaatacaa
    32161 tcattgcaag gcttatctct tagagctata atttcataac attttttatt gagtggatgc
    32221 tcaattgaaa accattcttt aaaggtatca ttggtttcaa tgattgtata atctctgtta
    32281 attacataaa gtgccatggg tatggcgttg ataagtgatg aatatttcgt ttcagaaata
    32341 ataattttat tctggtaacg gtattgcctg agcccattag caattagtcg tgtaataagt
    32401 tcaagggttg aaaattcatt ttcatctaaa atggtgtgat ttttaggata aattatatca
    32461 aatgaaccaa tagtttcttt ttcagcaatt agtgggaaag atactacacc tccaaaataa
    32521 ggtgattcaa taatttgttt ttgaatatca ttagtaagaa ttgctgctaa atctttgctt
    32581 gccattgaga aatcgcttac tatctgttca agggtgtata ccgttttgtt catgcttatt
    32641 gtatcaggaa aactaatagt atcagaaaaa gttatgtatc ctcgtaaaat tgaaaataaa
    32701 atactttttt taaatatact tcgatttgct ataagttttt taatattaat ggtggtactc
    32761 tctaaattga ggagatcttg atttatttgt ggaataaatt tttttaatgt atgcaattcc
    32821 ggaaatacta ataaatggtc actcccatca atattggata ggcatgagat attaaagact
    32881 ggtatatgta aaagataaat aatgtttttt aattttttta tatattctga tacagaatga
    32941 gtagcgttat gtaaattggc aatttgtaat aaatatttta ctggataagt attttcagta
    33001 aagcctatat cattgttcat cagaataaca cttaaataaa aaattataat ccgttagcaa
    33061 acaacaccat actaaatata aatgctttaa aaaatcaaca aaaatattca gtcaagaagg
    33121 attttttatg ggataaatgc ctatatgcaa ttaattttat tttgatacaa atttttgaat
    33181 gataaaaatt ataaaactga caagtcattt ctatttatcg aaatattaaa aattttcatt
    33241 aaaaaatatt tgcgaatatg atttaataat taaatgaggc tggggaataa tttccaaaaa
    33301 cagctcatcc aattctccat attcg
//
LOCUS       DAORYL010000025        33166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1093_length_33166_cov_12.880161, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000025 DAORYL010000000
VERSION     DAORYL010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 33166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33166
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1093_length_33166_cov_12.880161"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..1261
                     /locus_tag="PLV81_06555"
     CDS             257..1261
                     /locus_tag="PLV81_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOM09797.1"
                     /translation="MVKDIIVFYYLPVSIFISYCCCTIITTIIYAIKKNNVIVPVLIL
                     CIALMFVSLMYVYPFYVYFCIPHYVDTIVIIFIGIYCLLVAVPPYGDSIHYFHSNMYS
                     VIGKSIIILLIIIFTNNVYDQQKIIIPSVDMILSFSVLFVKPENSTCLRIHYPHRESF
                     LILVLIAIGYCILFMDSCILMPSLLVFAVLSVSIISFIAKFLIYLCNNSNQEYIGIHN
                     EQLYTTEKYKALIKIIKEHVNEYYNSPIDRYTIALQCEMHPDYISKVFKSEEGITINK
                     YIQIVRLEKAYHLLLYSDKKIIEVAFDVGYDNLATFYRHFVKHFKKSPQQIRKEQPDG
                     "
     gene            complement(1270..2661)
                     /locus_tag="PLV81_06560"
     CDS             complement(1270..2661)
                     /locus_tag="PLV81_06560"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOM09798.1"
                     /translation="MDAFFNPRSIAVIGASNKPFNLGATICEYLTFLNYDAKIYAINR
                     KGEDVKGCKGYRSLLEIPHDVDLAIILTPAPAVPDVMEECGKKGIQAVIIETAGFTEE
                     GEYGRKLQQQVNEIARQYNIRFIGPNCLGTLNAHNRFCCFFGARPGEYDGVFERPGDI
                     SYVIQSGGVGVLIMDSLRSDIVNVNKMMSIGNKEDIDEADCIDYFNTDGTKVIGMYLE
                     SIKNGKRFLEAAKRSSIPILVYKVGRTSEGAKAAMSHTAGMANNDTIFDAACRQVGII
                     RCKAISELHSMPKIFTTMPLLKGKRIAIFTNSGAFGGITSDLLVEAGFEIPTLSGELQ
                     QRISQCGKLYNVSNPIDLGPTLSLQTFIDIFTNLLSSDEIDGICAVPNVWQQVVIDGI
                     LELMKLCKEYDKPAGIYIPNAVDRILKVRTAYSIPSFESPEEAVRALQISYQYYCASR
                     KKSFTQSKTSAVI"
     gene            complement(2952..4403)
                     /gene="putP"
                     /locus_tag="PLV81_06565"
     CDS             complement(2952..4403)
                     /gene="putP"
                     /locus_tag="PLV81_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HOM09799.1"
                     /translation="MITTFITFGLYLVLLLAIGVYYYNKSHDISDFILGGRKLGKFVM
                     ALSAQASDMSGWLLMGLPGALYTKGMPAIWIAVGLLLGTYCNWKFVAKRLRIYTEITD
                     SITLPTFFEDRFKDPTGLLRIISALIILIFFTIYLSSGFVASGKLFESMLGIEYHIAV
                     LIGAGIILFYTSLGGFFAVSVTDVVQGSLMFIAIIVVPIVSMKHIGSFNTITHAMQKA
                     SLPINPFTSVTLLSVVSTASWGLGYFGQPHILARFMGIHSHHDIKSARQIAMTWVTIS
                     LCGASFIGIAAVYLFPELSQHNAEKVFIFMIQKFFTPWIGGILLAAILSAIMSTADSQ
                     LLVCSAALTEDFYQRIIKRNASQKELIHIGRITTMLITIIAALFAMNPENTVLGLVAY
                     AWGGFGAAFGPIVLFALFSKNTSWQSVLGGMLAGTIALVSWNFLGLSNYCYEIVPGFI
                     ANVITIMVLNRIYPPQNSTINTEFATMKKEIQS"
     gene            4720..5049
                     /locus_tag="PLV81_06570"
     CDS             4720..5049
                     /locus_tag="PLV81_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOM09800.1"
                     /translation="MIELTDKGNGTFVVKIIMQEVHTLDVPDLKEKLQQAIVNKGIKR
                     MVVDLSDVKMITSSGIGIFLNINQNLKSQFRLAAPTPEVQKVLELTKVTSMIKVFNSV
                     EAALQSF"
     regulatory      5302..5405
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            5479..6642
                     /locus_tag="PLV81_06575"
     CDS             5479..6642
                     /locus_tag="PLV81_06575"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOM09801.1"
                     /translation="MKHDITLDTSVGIVQTQYYTFAEPPDEMVLASGKKLGPITLAYE
                     TYGTLNESKSNAILILHALSGSAHAAGYHTEADAYPGWWDHYIGPDKAFDTSKYFVIC
                     SNVIGGCSGSTGPSSVNPHTGKEYALDFPIVTIQDMVNAQRYLLDYLGIEKLLAVAGG
                     SMGGMQALQWSISYPDRVQSVIAIATAASLSAQGIAFHEVGRQAIMRDPNWNNGNYYH
                     ATSPSNGLSLARMIGHITYLSEKLMHEKFGRRLQQADIFKFQFDLEFEVESYLHHKGN
                     DFVQRFDANSYLYITKAIDYFDLKNDFGGDLANAFVNVNSDYLIISFSSDWLYPTSQS
                     REIVRALRMNSKNVVFTEIQSDKGHDTFLLPNEQLEHNIANFLKREFEKVHKK"
     gene            6639..7262
                     /gene="metW"
                     /locus_tag="PLV81_06580"
     CDS             6639..7262
                     /gene="metW"
                     /locus_tag="PLV81_06580"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HOM09802.1"
                     /translation="MKEYRVAYDLILDLIPPGSRVLDLGCGDGLLLKLLQEKKQVKSF
                     GVEISPEGVSQCVEKGLYCYQGDIDEGLSDYKDNSFDYVIVNQTLQSTKKPDVVIRES
                     LRIALNAVFSFPNFAHYSLRLYLSLYGRMPKNKLLPYEWYETPNIHLLTIKDFRDYCR
                     DFKYPIKKEMHFSTIRSRSVIIPVMPNLFAEYGFFILDGQPFTSATL"
     gene            7358..9364
                     /gene="uvrB"
                     /locus_tag="PLV81_06585"
     CDS             7358..9364
                     /gene="uvrB"
                     /locus_tag="PLV81_06585"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOM09803.1"
                     /translation="MPQFKVVSAYKPSGDQAQAIEKLAEGIASGYKYQTLLGVTGSGK
                     TYTMAKVIEKVQKPTLVLTHNKTLAAQLYREFKEFFPNNAVEYFVSYYDYYQPEAYVV
                     SQDLYIEKDASINDEIDRLRLKATSSLVEREDVIIVSSVSCIYGIGNPESFEKNHIRL
                     AVGDMVDRQELLMKLVSIHYDRNDIAFTRGTFRVRGDTVEVFPANLQDALRIVFFGDE
                     IEALYKINPVTGARQETLEKAYIYPAKHYVSSKEEMEETIRMIEIELEHRIKEFMDAG
                     KLVEAQRIEMRTRYDIEMLKTVGYCNGIENYSRIIDRRQPGQPPHCLLHYFKSNYLMF
                     IDESHVTIPQVRGMYEGDRSRKVNLVEYGFRLPSALDNRPLKFDEFEAMIDQVVFVSA
                     TPADYEIEKSDQVVEQVIRPTGLIDPDIEVRPATNQVDDLIGEIKKRVAANERVLVTT
                     LTKKMAEDLTEYLTEVGLKVRYLHSEIETIERVEIIRDLRKGEFDCLIGINLLREGLD
                     IPEVSLVAILDADKEGFLRSTRSLIQTAGRAARNINGKVIMYADTVTRSMQEAIEETN
                     RRRALQMEFNKKHKITPRSISKEIVDIIEREYHSDDVFADIVAEYTQSYRTGNIEELK
                     KVRDKIKQDMLEAADNLEFEKAAILRDQLFDIEKKIGLYSKVAQ"
     gene            9386..10225
                     /gene="nadC"
                     /locus_tag="PLV81_06590"
     CDS             9386..10225
                     /gene="nadC"
                     /locus_tag="PLV81_06590"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOM09804.1"
                     /translation="MEIPSYDELSPLIEMALKEDIGSGDITSNAIFDEYHFTRARIYS
                     KSEGVFCGGFLIEYIYHTMDPSVSVTLYRNEGAPVKEDDVVADIEGNVRTLLSGERTV
                     LNFLQRTCGIATRTARMVSIVRDTNIKILDTRKTLPGFRLLDKYAVWCGGGTNHRMGL
                     FDMVLIKDNHIKAAGGILQAVEMVKKVYDNKYVIEVEVESLNQVQEAVEAGADIIMLD
                     NMSKDRIALALSIINGKAKVEVSGNMDEHKLKEFSDLPIDYVSIGALTHSVKAFDLSM
                     RLL"
     gene            complement(10227..11066)
                     /locus_tag="PLV81_06595"
     CDS             complement(10227..11066)
                     /locus_tag="PLV81_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018394885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOM09805.1"
                     /translation="MLIGIVGAGIMGTDIAHLAIENNYEVILHDIDESQLKHAYETIT
                     GHLDRYVEEKRLEQSQRGKIISLLKLHKNIKDLHKADIIIECIPENLECKKNVFKSLD
                     AICKAGTILASNTSSLPITSIASATKRPEYVIGIHFMNPARAMRLVEIVGGIATTRET
                     IESAKSFVKSLGKTPVESKDYPGFVLNRILMPMINEAIFTLYEGAASAETIDKTMKLG
                     LNLPMGPLQLADLIGLDVVLAIIEELYEGFGDPKYRPCPLLKKYVEAGYLGKKTGRGF
                     YSY"
     gene            complement(11078..12043)
                     /locus_tag="PLV81_06600"
     CDS             complement(11078..12043)
                     /locus_tag="PLV81_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOM09806.1"
                     /translation="MSKTIVFLDASTVDFGDIDFTPIASLGTFITYPVTKPHETISRS
                     KDADIIITNKVVFYADEIAQLHKCSLIAVAATGYNTIDIKAAKNKNIAVANVPGYSRE
                     AVAQLTLCFILALANNLIKYNAATHDGSWSRSPIFTMGNWPTMCINDKVLGIMGYGDI
                     GKRVAKLAQAFGMKVIALKRENSYTDTSVERYPLEEFCTMADFISIHMPLNESTHHMV
                     NKDFLAMMKPSAYIINMARGPIVDTKALAQALHNNVIAGAALDVLEQEPPDINEPILS
                     APNCIITPHIGWASRETRQALINEIAENIKAFLNGTKRNIVNETV"
     gene            complement(12100..12579)
                     /locus_tag="PLV81_06605"
     CDS             complement(12100..12579)
                     /locus_tag="PLV81_06605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOM09807.1"
                     /translation="MVEIHPLNNFPNYAPVLAYWSYNQWYSKRDIPFTALVQSYIERA
                     RNTTVPLAWVAINEKMLPVGMASLKNNDLWSRTDLNPWLASLYIVPEYRFQGIGDRIV
                     KAIIAKTKELGYKTLYLFEGHNDTVNLIQYYTKRGFSVLEEAIDNDGKPTTIMYINL"
     gene            complement(12646..14274)
                     /locus_tag="PLV81_06610"
     CDS             complement(12646..14274)
                     /locus_tag="PLV81_06610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HOM09808.1"
                     /translation="MKRFIEENTKEQFDCIIVGGGITGAAVAYEAASRGLTVALLEKN
                     DFGWATSAATSKLIHGGLRYLNYFEFGLVRESLRERRILENIAPNLVYPFPFMIPNYN
                     RLENNRWVLKIGMILYDILSYDKGFTWDKSKKIPMHFTLSKEKALQEEPNIRPEGLTA
                     ATVYYDCQSIFPERLTLAFIKSAVQYGAKVANYAEVVNFVYTNNSITGVIVHDKLTGR
                     EVTINGNLIINCGGPWADIILKKAENGKTKHQIRRSEGIHIITKPMVNTYAVGLMPRK
                     GRHFFLIPWRGYSLIGTTDKEYIGNPDEYTVTKQSIQELLDDVNSAFGDGNLTFKDIR
                     FAYGGLRPLVEDQVEGTYQSSRRYEIYDNAEEGVNGLITVEGGKYTTSRNLAVNVMKL
                     VEKKIQRKLPPSVTHTQYLAGCTIKDLEAFFNTVYNSYPSFNKNTLTVLVKYYGSELD
                     ALMSIANSNKKYREIVTHDGEILAEVIYAIRHEMARTLSDIVFRRTGICTAGNPGNDV
                     LNLVAETAAKELKWDEKELKRQLKSVQQRLQLPK"
     gene            complement(14279..14992)
                     /locus_tag="PLV81_06615"
     CDS             complement(14279..14992)
                     /locus_tag="PLV81_06615"
                     /inference="COORDINATES: protein motif:HMM:NF019456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HOM09809.1"
                     /translation="MKKNTLGKIQKNTLHQQIVSLLAKRIINQMQPGDKLPSERDIAA
                     DLDVNRATVREALKKLETLGLIEIRHGEGIFIKDYLSSGNLELFKMMIYLNDTIPLSI
                     LQNLLEIRRIIVPQMAANASENITREELQQFEAIVTSHDSILEKDLAVHQLIAKASRN
                     LLYIFILNFFNQIFRDFGYLYFENPDNQKRSELFHRELLEAFKAKNSKKAYTITHSVL
                     EYTEKRIFAYYSQIYNNKG"
     gene            complement(15079..16254)
                     /gene="galK"
                     /locus_tag="PLV81_06620"
     CDS             complement(15079..16254)
                     /gene="galK"
                     /locus_tag="PLV81_06620"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOM09810.1"
                     /translation="MSSIIDTVIAHHREYFPRSTDCALFASPGRINIIGEHIDYNGGN
                     VLPAAIDKHIYICISRNNDGIIRFVSANHKETGEVAINNLHSPQTKAHAAHAWWVYPY
                     GACVLSNIEFDCGLDFSFYSTLPVGAGMSSSASITVGTLYALYSLNNITIKSKDIALQ
                     AQKVEWDFAGVKCGIMDQMAIINGKKDNAIMLNTQTMEFSYIPVDTSTVRFILVNSGV
                     KHSLRESAYNDRRHECERALLTLKKAGFAIQNLCDCSVDDLIQIQNILPQTEYKRTYH
                     VIYENHRVHQFAREIQQYNLKKAGELLYKSHQSLSVYYEVSIPLIDEMVEWASDIDGV
                     YGSRLMGGGFGGCTISMVALYAVEHFTRTIAQKFKKRENRIPQIYECSIEDGTRRIE"
     gene            complement(16260..17123)
                     /locus_tag="PLV81_06625"
     CDS             complement(16260..17123)
                     /locus_tag="PLV81_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09811.1"
                     /translation="MKHSADVPSHRFRSTINNLALYALENELTNDLIAREIEKRFATK
                     KTGKKDKIMKDVLQSCYRLVWDSTLPFENSIIIKEVKDEHTQIIEATTAITLAQCIIQ
                     IMKHFDVYPEKNKPLPQNFYSLCVDMLLDGPLAHYDPDLLVIYCKQTVIMLKAAGITN
                     EHSVDTINTGELYRRLFLAFWNKCNWKNLFPSGGYISNDIKNNRQLLLDIILSSNKPV
                     QLDSIARTYFELTGIANPYDLFAISLLDFSVITWLSFFGIIEYVHTAADMPVTIALTR
                     SAYNLVHLISQ"
     gene            17540..18460
                     /locus_tag="PLV81_06630"
     CDS             17540..18460
                     /locus_tag="PLV81_06630"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOM09812.1"
                     /translation="MWLLSLIDATHNDVKEIQENSKDILHFLAEEIGERTLRKYDNLN
                     RTRHYIHDYLKKYNDRVYEEKYLVNGMEVGNVIAEIPGFEDNDEIIIIGAHYDTIEDT
                     PGADDNGSAIAGLLELHRLLSQYSFRKTLRFVAFTLEEPPFFSGPYMGSMVHAKGCKE
                     RDEHIDLMICLEMIGYAGSRIHQDYPLSSMQKEYPPYGNFLAVAALPSYAPYAYKWKR
                     EYNRFAKNKIFDMIGPASIPGINLSDHYSFNKYSFPAIMITDTAFYRNKNYHTEGDTY
                     ETINFKFLAEAIFNTFMAVKTIANERDILE"
     gene            18457..19005
                     /locus_tag="PLV81_06635"
     CDS             18457..19005
                     /locus_tag="PLV81_06635"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOM09813.1"
                     /translation="MSDFITIPIQDSHRLVNAGNVILVSAQSHEKKTVTTVAWHMPVS
                     SQPKLLSCALANKRYSLELIKESRCFCINIPEFKLLDAVVFCGTYSGRNVDKCKEANL
                     TAVPCSKIHGFFIDECVAHIECELYSMIEAGDHTIVVGNVTGAMAQKHLFTPDGVIDI
                     NKVQLIHHLGGTHFGILKSQET"
     gene            19010..19213
                     /locus_tag="PLV81_06640"
     CDS             19010..19213
                     /locus_tag="PLV81_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007043766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HOM09814.1"
                     /translation="MECKIERNLSLCTCSYDPCSRKGLCCDCIAYHLKKRQLPGCAFP
                     KDAERTYDRSFEHFARLVQTGKV"
     gene            complement(19215..20105)
                     /locus_tag="PLV81_06645"
     CDS             complement(19215..20105)
                     /locus_tag="PLV81_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOM09815.1"
                     /translation="MVSWMSANDIRFIIVLLIFHMGCVPPAYRQPQDIHKKNTQKTYQ
                     QFNYNEDDSTPLITDEDNYYIKNDYYSTQPVYYTTPQVESETSYHNSQSNENNNSKQS
                     SSKGTWHIIKKGETLYRLSKQYNTTVDAICEANNINNFTTIKVGQKIFIPSNDFVNNS
                     SSKKSSPNQIPQKKFDSSFSFIWPVPFVMTYTRDHGGVRPIGIIITSRPKSKVVASAE
                     GTIAKIGTMRGFGNFVVIMHKKDFYTIYSKLDAITVKEGQFVVKGTTIGFLSDSNPSL
                     HFQINHSGKPLDPLHYLAKK"
     gene            complement(20212..20631)
                     /gene="nikR"
                     /locus_tag="PLV81_06650"
     CDS             complement(20212..20631)
                     /gene="nikR"
                     /locus_tag="PLV81_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOM09816.1"
                     /translation="MNNVIRFGVSIDEKLLDRFDALINEKGYVNRSEAIRDLIRDMLV
                     KEEITDPNAEVIGTLTLVYSHDVREIADKLNDLQHDNYKYIISSVHVHLDEHNCLEVL
                     LLRGTSSQVKKIADNLLAIKNVRHGKLTITTTVDYHE"
     gene            21009..21404
                     /locus_tag="PLV81_06655"
     CDS             21009..21404
                     /locus_tag="PLV81_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09817.1"
                     /translation="MPVFSNTEKMYEVLGELFNALLDDTTAREKFLESDIIIKFDIHD
                     PAGQIWISNEDGGKVICGSTQLKPTIEMTLSGDSCHKFWLQELTMPVALAKGLIKAKG
                     PMPKVIKLLPLLKPAYAAYPEIAKKHGLI"
     gene            21417..22094
                     /locus_tag="PLV81_06660"
     CDS             21417..22094
                     /locus_tag="PLV81_06660"
                     /inference="COORDINATES: protein motif:HMM:NF021439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HOM09818.1"
                     /translation="MSIERSMKQFEEDRKAAVKIAAHLLLASATTTPRVGGVGECTVH
                     IIDDECKIEDLCQAIEKMAKENERWEFFTRDAMMLRDADAVLIITSLRCYTDPADTNC
                     NMCGKLTCEYLKESEKLPDDPGIAYKGPLCIFRANNIAYAVDGIVSLARNLGIDYGVF
                     WSAGAAAMRMGIVPKNTGFCLAVGISVTEKSPFRDIPLRYDEINEKTMNDRIIKRLWP
                     QFRSIYS"
     gene            22174..22824
                     /locus_tag="PLV81_06665"
     CDS             22174..22824
                     /locus_tag="PLV81_06665"
                     /inference="COORDINATES: protein motif:HMM:NF021439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HOM09819.1"
                     /translation="MPAIEMNELIQNGLNRAVELMALAAYNSFRFGKRNTIKMVSLSI
                     DDLKEIAEFCYSLGDMSPLAARDGRFLMELVKEPCAVLLIGDKRKSDFNYNCGACGYR
                     TCAELNKAEEVEALTARGPSCQFKNLNINIAAGAAASMAWRLGLHCRVFSTIAFAAFA
                     LNKLEGIDIATSVAVSAAKVDPFFDRHQYWTKEHWDEIFAKEFPTFTRGFIGAIEE"
     gene            22841..24454
                     /locus_tag="PLV81_06670"
     CDS             22841..24454
                     /locus_tag="PLV81_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOM09820.1"
                     /translation="MATKAHLNPVIASHLVEIKADEIPDTLVYIFEQGDAGDERCTYK
                     DLYENSNKIARLFLDNGIDKGDTFAVFMKNYPEFVYALLAGTTIGAIMVPIDPRSRGD
                     KLRFLLHNCNAKAVIVEDELLSSLEEVLPNLPAIKLVSVVYKKGHAIPVADKYHALNE
                     TIEKTTWDTVDQMIMDVRHPMQIIYTSGTTGDPKGVAIRNNRTGLFNILNSIVWRYQK
                     DDILYNGLSLTHGNAQAVTLFPALSMGITAVFSPRFTKSRIWDICRKYGCTSFSLLGG
                     MMAGIYNEPEKPDDADNPVKVVISAGTPTTIWEAFEKRFNVKILEWYGSVEGGFAYKP
                     IGKGPIGSFGKPVPGVMEFKIVDENDNEVPVGQTGELICRLLKGETKVDYLGKPEESK
                     EKTRGGWLRTGDMVHRDAEGWYYFDYRKGSELRRSGDFIQPEYIETVIGKHPDVSEVS
                     VYGVPAASGAPGESDLVAAIACFEGKSVDPKSFYEYLKKNLEANFIPSYLQIVPEIPK
                     TITEKALTRVLKEDFLKGLGTIYKYEDYK"
     gene            24493..25737
                     /locus_tag="PLV81_06675"
     CDS             24493..25737
                     /locus_tag="PLV81_06675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOM09821.1"
                     /translation="MLYTELNIELTDEHQHLKEQVHRFSMEVLRPASLELDKLPAEEV
                     VKKGSIYWDCMAKMYQNGYHTVLISDEYGGLGLDPLAVHIFWEELAYGSVGFAVSLGC
                     SCFAAFYATMLAEDKLIEKFVIPFVNCKDASVMSCWGITEPDHGSDNLAPRTSFFKDA
                     SLTQQVKAHKKNGEWIISGQKSAWISNGPVASNCALFLNLDPSKGLSGGGICLIDLNQ
                     DGVTRGKPLDKMGQRELPQGEIFFDNVVCPEEYMIVDPESYEAMTELTLGHANATMGA
                     YFTGLAQAAYDMTLQYCTERIQGGKRLCDHQWVQKKLFDMFVKVETARAFSRAAMIYN
                     MNTTPPDVKYSIASKVYCTEAAFQVTSDAIQLFGGYGLSKEFPIEKLFRDARAGMIED
                     GSNDTLSMTAGNIIIKEKLGDE"
     gene            25730..26971
                     /locus_tag="PLV81_06680"
     CDS             25730..26971
                     /locus_tag="PLV81_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOM09822.1"
                     /translation="MSNVHIIGSGMIRFNKYPDETVRSMAEKAIKLALEDAGIDKKDI
                     QAGFFSNTFWGMFSGQHSIRGQVIFRGMGIDKIPVVNVENACAGGSSALHLAYTGIKA
                     GMYDVAIAVGSEKITNPNKALSLGAYASCMDVENFEKHINMIIEVSKSFKITIPHDES
                     KPGEGRSVFMDAYAVGARWHMDRFGSTQHQLAVICSKNHLHGSLNPLSQYQHKMTVEE
                     VLADKLVAWPLTRAMCAPVGDGAAATIVCSEKFLKKLTGARPVKIIASVMGQGEDRDL
                     DAPDIGERLSRLAYEQAGVGPGDISLAELHDATSWGELHQTESMGFCPVGEGGPFAES
                     GATTLGGKKPINTSGGLECRGHPIGASGLAQIHELVLQLRGEAGARQVENARLGLAEN
                     GGGNIGVEEAAMCIHILEKVR"
     gene            26990..27799
                     /locus_tag="PLV81_06685"
     CDS             26990..27799
                     /locus_tag="PLV81_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOM09823.1"
                     /translation="MAINFNNRWVVITGASSGIGKALAECFAREGAHLILGSLPGEKH
                     ILESIRDNLQKQHAIQIVTIYQDFADPDGAVKFYDQCKKLQKQIYCLVNNAGIMAYGY
                     FHELSHKQQKTLININLMAYFSLMRLFIEDMIQQEEGVIINISSVSAFQPTPHHAVYG
                     AVKAFVQSLSEAVDQECRGTGISVHTINPSYTDTPLLKKNNFPNKIWWYRISGLAKPE
                     DIARKAIKAVKQGKRLYVPGVMNALIHLFLPRLLPRKLSNAIAHIVLRQER"
     gene            complement(27808..29352)
                     /locus_tag="PLV81_06690"
     CDS             complement(27808..29352)
                     /locus_tag="PLV81_06690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOM09824.1"
                     /translation="MKLFILIFYGLILLLLSIFGAHRYYTLYLFLKYAKKKIQPKSMF
                     KSLPKVTVQLPIFNEQYVVERLINTITQLDYPKKKLEIQVLDDSTDETSIIAERICKL
                     KRKEGFNIKYIHRTDRTGFKAGALENGLGLTDSEFIAVFDADFLPNPDFLQKTIHYFT
                     DPEIGMVQTRWDHINRHYSLLTECQSILLDGHFMIEHTARNRSGRFFNFNGTAGIWRR
                     QAIIDGGGWQHDTITEDLDLSYRSQMKGWKFLYLPDVTVPAELPIEISAFKAQQFRWA
                     KGSIQVFKKLYKTILHSHLPLKVKLESFMHLGANFAYLLMVFLTLLMPVALWVRYYGG
                     FKKFAYLDLPIFLLATLSVMIFYYFTELIVLYQNKNIYDSKIKPLLYLPMVLSLGIGL
                     SINNSKAVIEALLDKKSDFNRTPKYNVVALNRINAIDHVKTLLKNKYSLHTINFVSII
                     EFVLGLYMTLAVYFAFDKNLYISIPLIMLFQVGYLYTSLFTIINSITHQVLMYISLFR
                     RGLANE"
     gene            29528..30178
                     /locus_tag="PLV81_06695"
     CDS             29528..30178
                     /locus_tag="PLV81_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09825.1"
                     /translation="MTQATVQALQGLRDLSMVKWYIIPLLAIVFYIYTKEIKEARKTG
                     DWDAVFAGATIFGVDFFNETWNGWVMYLSQRSAVWTTPGDTALRTMVGWNIEIMFMFT
                     LLGIIFYHTLSGKNSKILGIPEKWFWAVVYAAFCVFVECLLNIGGHLVWEYEYWNLSF
                     KGVWLIFLFGYFHFFVFAIIVIGLSTIKKKIIAVSCIYAVPIIMNIVALGILGWNY"
     gene            complement(30172..30753)
                     /locus_tag="PLV81_06700"
     CDS             complement(30172..30753)
                     /locus_tag="PLV81_06700"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HOM09826.1"
                     /translation="MEAIEACILAGGKSSRFGSDKTLHLFKGRPLIEHVLHSLKPLFQ
                     TISIIANDTDKYNYLTIPVYPDIIPGLGPLGGIYTALKCSATDYIFVCAADMPFLNQE
                     FIAFMLQIPHMYDCIIPCWAGNTEPLHAVYSKQCIPHIEKLIKEKKYKISFLFEQVRM
                     RYIMDDELIFYAEDPSELFYNINKQSDIPPSFQ"
     gene            complement(30757..31614)
                     /locus_tag="PLV81_06705"
     CDS             complement(30757..31614)
                     /locus_tag="PLV81_06705"
                     /inference="COORDINATES: protein motif:HMM:NF016131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HOM09827.1"
                     /translation="MPMIHHTEHLEQRKESLLNNSMFSEDLFALYRSLFSLQFQSLQH
                     LASIITDSKLIVDTTQYPMIQPHTLQFTDEIIAGIKKMFKNIAALITQYQTAYSFDTL
                     LANLDDNPELYKDIITALLSKDSTFEAIAHNNKLDTDELIFVTVNAYKPLFIALKDAA
                     LQDITQFPDYSEGLCPFCGFLPDMAKIVEPKNNKRFLHCALCECEWEYKRVKCVACGN
                     ENTDTLGYYSYEPDEKYRFDYCNKCNTYIKTIRITKQHDESQFDLTVENLITNFLDAS
                     ALQMGYTRQ"
     regulatory      31758..31882
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            32137..33166
                     /locus_tag="PLV81_06710"
                     /pseudo
     CDS             32137..33166
                     /locus_tag="PLV81_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941441.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT        10202 a         5733 c         6927 g        10304 t
ORIGIN      
        1 taatgagtta gttatatata gtattgatta taagtcattg ctatagtggg aaaggtgata
       61 ttccttttaa atattataaa tataattgac aaattatata aagtttattg ttttaattca
      121 aaataaagat tataaatgaa tcaacaaaaa gataaaaatc aaaaataaat aaaagttata
      181 actatagatt attacaacag ttatgtttta aataaaagat aaaagataaa aaaagaaaaa
      241 ggaaggaatt gttgccatgg taaaagatat tattgttttt tactatcttc ctgttagtat
      301 ttttatttcc tattgttgct gtacaataat taccacaatt atatatgcaa taaagaaaaa
      361 caatgtaatt gtgccagttt tgatattatg tatagcactg atgtttgttt cattaatgta
      421 tgtatatcct ttttatgtat atttttgtat acctcattat gtggatacca ttgtaatcat
      481 ttttattggg atttattgtt tattagtggc agtaccccct tatggggata gtatccatta
      541 ttttcatagt aacatgtatt cggttattgg taaaagtata atcatattat taattattat
      601 ttttacaaat aatgtttatg atcaacaaaa aattataatc cccagtgttg acatgattct
      661 ttcttttagc gttctttttg taaagccaga gaattcaaca tgtttaagaa tacattaccc
      721 gcatagggaa tcattcctga tactggtttt aatagcaatt ggttattgca tactatttat
      781 ggatagttgt attctgatgc cttcacttct tgtttttgct gtactatcgg tatctataat
      841 cagttttata gcaaagtttt taatctattt atgtaataat agtaaccagg aatacattgg
      901 aatacataat gagcaattat atacaacaga aaaatataaa gcattaatca aaataattaa
      961 agaacatgtt aatgagtact ataactcacc cattgacaga tataccattg cactgcaatg
     1021 tgaaatgcat cctgattaca tatctaaagt cttcaaaagt gaagaaggaa taaccattaa
     1081 taaatatata caaattgtac gcctggaaaa agcttatcat ttattattgt attctgacaa
     1141 aaaaataatt gaagttgctt ttgatgtagg ttatgacaat ttagccacat tttaccgaca
     1201 ttttgtaaag cactttaaaa aatcacctca acaaattaga aaagagcaac ccgatggatg
     1261 aaatgactgt tatataacag cactggtttt tgattgggtg aatgattttt tcctgctggc
     1321 acaatagtat tgatatgata tctgtaatgc acgaaccgct tcctcaggtg attcaaaact
     1381 gggaatagaa tatgcagtgc gtacttttag tatacggtca actgcattgg gtatgtatat
     1441 ccctgcaggt ttatcatatt ctttacataa cttcatcagt tcaagaatac catcaataac
     1501 aacttgctgc catacattag gtactgcaca gatgccatca atttcatctg acgataaaag
     1561 attagtaaaa atatcaatga atgtttgcag tgacaacgtt ggccctaaat ctattggatt
     1621 tgaaacatta tagagtttac cacactgaga aattctttgc tgtagttcac ctgatagtgt
     1681 aggtatttca aagccagctt caacaagcaa atcagaggtt atgccgccaa aagcgcctga
     1741 attggtaaaa attgctatgc gtttgccttt gagcaaaggc atggtggtaa agatctttgg
     1801 catggaatgc aattcactta tggctttaca gcgaataatg cctacctgcc tgcatgctgc
     1861 atcaaaaatg gtatcattat ttgccatgcc agcagtatgt gacatggcag cctttgcacc
     1921 ttcgctggtc ctgccaactt tatataccag aatggggatt gatgagcgtt ttgcagcttc
     1981 aagaaatcgt ttaccatttt tgatgctctc aagatacatg ccaataactt ttgttccatc
     2041 ggtattaaag taatcaatac agtcagcttc gtcgatatct tctttattgc caatgctcat
     2101 cattttattg acattgacaa tgtcgctccg caatgagtcc attatgagta cgccaactcc
     2161 gccactctgt ataacatagg aaatatcacc ggggcgttca aacacaccat cgtattcacc
     2221 gggacgtgct ccaaaaaagc aacagaaccg gttatgtgca tttaatgtgc caagacagtt
     2281 tgggcctatg aaacgtatat tgtattgcct ggcaatttca tttacctgtt gctgcagttt
     2341 tctcccatat tcgccttctt cagtaaaacc agcagtttca atgataactg cctgtatgcc
     2401 ttttttccca cattcttcca ttacatcagg cacagcagga gcaggagtaa ggataatagc
     2461 taaatcaaca tcatgaggta tttctaaaag gcttctgtag cccttacagc ctttaacatc
     2521 ttccccttta cggttaattg catagatttt tgcatcataa ttaagaaatg tgaggtattc
     2581 acagattgta gcacctaaat taaatggttt attggaagca cctattacag caattgaccg
     2641 tgggttaaaa aaagcatcca tagatagctc cattataatg tagtatttgt atgacaactt
     2701 acaaaatatg gtatttttat caataaaaaa aatatagtta ttttatatta ttttttataa
     2761 ttttttgacg aaggggaatt attatatacg gtatatattg cctaaatgtt tcagaaaaaa
     2821 atgatagcaa ttatcttcat cgttataagc aaaaaaagat gaataatgca ttattgtata
     2881 ttaaaaagca tttttatgag tgattatttc aatataatac ccttttatat ttttaatacg
     2941 tgtattgatt gttatgattg tatctctttt ttcattgttg caaattcggt attgattgtg
     3001 gaattttgtg gaggatagat tctgttaagt accataatgg ttattacatt cgcaataaat
     3061 ccaggaacta tctcataaca ataattactt aatcctaaaa aattccatga aaccagcgca
     3121 attgtgcctg caagcattcc ccccagtacg gattgccatg atgtattctt tgaaaaaagt
     3181 gcaaaaagca ctatagggcc aaatgcagca ccaaaaccac cccatgcata ggctaccagt
     3241 ccaagaactg tattttcagg attcatggca aagagtgccg cgatgattgt gataagcatt
     3301 gtggtaattc ggccaatgtg aattaattct ttctgtgatg cgtttcgttt aatgatacgc
     3361 tggtaaaagt cttcggttaa tgctgctgaa cacaccagca attgtgaatc ggctgtggac
     3421 atgattgctg aaagtattgc tgcaaggagt attccgccga tccagggtgt aaaaaatttc
     3481 tgtatcatga aaataaaaac tttttctgca ttgtgttgcg atagttccgg gaatagatac
     3541 actgctgcaa tcccaatgaa ggaagcacca cataatgaaa tggttaccca tgtcattgcg
     3601 atctggcggg cactcttgat atcatggtgt gaatgtattc ccatgaaacg tgccagtata
     3661 tgcggttgac caaaatagcc aagcccccat gatgcagtag acactactga gagtaacgtt
     3721 actgatgtaa atgggttaat tggcagagac gccttttgca tggcatgagt tatagtgtta
     3781 aaggatccaa tgtgcttcat ggaaacaatg gggacaacaa taatggcaat aaacataagt
     3841 gagccctgaa caacgtcggt aacactgaca gcaaaaaaac caccgaggga tgtgtaaaaa
     3901 agaatgatgc ctgcaccaat cagtactgca atatgatatt cgatgcccag catggactca
     3961 aatagttttc ctgatgcaac aaatccagaa gaaagataaa tagtaaaaaa tataaggata
     4021 attaacgctg atattatacg aagaagccct gtggggtctt tgaagcgatc ttcgaaaaat
     4081 gttggcagtg taatagaatc agtaatttcg gtataaatac gaagccgttt tgccacaaat
     4141 ttccagttac agtatgttcc aagcaaaagt ccaactgcaa tccatatagc aggcatgcct
     4201 ttagtataca atgcacctgg caatcccatg agtagccaac cactcatgtc actggcctgt
     4261 gctgataatg ccatgacaaa tttgcctaat ttcctaccac ccagaataaa atcagagata
     4321 tcatgtgatt tattataata atatactcca attgccagta atagaaccag atataatcca
     4381 aaggttatga aggttgttat catagcctgc tatcctgtat taattaaaaa acttatcaat
     4441 gattgcaatg tatattcttg attgtcaatg taatatcagt atttatttgt taactttttt
     4501 aaggaaagaa atattttacc acccacacta tcgaaggcat cagtggtata tatttaatag
     4561 tagtgttcta tcaagcattt aaggtagtat tactgatgca atggataaaa aataatatat
     4621 aaattaacaa cataagaaat caattttttt attgactaaa aaaaacactg tgactattat
     4681 ttcaaatgat tgagcacatt acagaaaaag gtgagcggta tgatagaatt aacagataaa
     4741 ggaaatggta cattcgttgt aaagataatc atgcaggaag ttcatacatt agatgttccg
     4801 gatctgaaag aaaagcttca acaggctatc gtcaataaag gaataaagcg aatggttgtt
     4861 gacctttcag atgttaagat gattaccagc tcaggtatag gtatttttct aaacatcaat
     4921 cagaatctta aatcgcaatt caggttggct gcacctactc ctgaagtaca gaaggtgtta
     4981 gaattaacaa aagttacatc aatgataaaa gtatttaatt cagttgaagc agctcttcag
     5041 agtttttgat gtaaatggaa taatatactc tcgccactga aagcagcgtg agtgtataca
     5101 ttcataatca attataaaaa acatgaatac tgttttcctt tttattacta attttgaaaa
     5161 tatattttga tttcatgccg tattaaaatt tattttatca ttatcggtaa tgtgctatat
     5221 ttgtgctaaa ttagtatggt gatatattcg atatagtatt ttgagttttt attatagcac
     5281 gattataatt ttaatcgtac tatcttatca agagtggtgg agggactggc cctgtgaagc
     5341 cacggcaacc ggcacaggta aaagtcggtg ccaattccag cggaagaaat tccggaagat
     5401 gagataggca actggtaacc gcttattcca aacttccggt aagcggtttt ttattttaca
     5461 taggagatcc actgttaaat gaaacatgac attacattag atacatccgt tggcatagtt
     5521 caaacgcagt attatacatt tgcagaacca ccggatgaaa tggtgcttgc ctcaggtaaa
     5581 aaactggggc ccataacgtt agcgtatgaa acatatggaa cattgaatga gagcaaaagt
     5641 aatgcaatat taattctgca tgccctttca ggatctgcac atgctgcagg ctatcacaca
     5701 gaagctgatg cgtatcccgg gtggtgggat cactatattg gtccagacaa agcatttgat
     5761 accagcaaat attttgtcat ctgttccaat gttattggtg gttgcagtgg ttctaccggc
     5821 ccctcatcag taaatccaca cacgggtaaa gaatacgccc tggattttcc catcgtaacc
     5881 atacaggata tggtaaatgc tcagaggtat ttacttgatt atctgggaat tgaaaagctt
     5941 ctggcagttg ccggtggctc aatgggtggc atgcaggctt tgcagtggtc aatatcatat
     6001 cctgacaggg tgcaatcggt tatagcgata gcaaccgcag cgtctctctc ggcacagggt
     6061 attgccttcc atgaagttgg caggcaggct atcatgcgtg acccaaactg gaataatggg
     6121 aattattacc atgcaaccag tccatccaat ggactatcgc ttgcccgaat gattgggcat
     6181 atcacatatc tcagtgaaaa gctcatgcat gaaaaatttg gacgaagatt acagcaagcc
     6241 gatatattta aatttcagtt tgatctggag tttgaggttg agtcatacct gcaccataag
     6301 ggaaatgatt ttgtccagcg atttgatgca aattcgtatt tatatattac caaagctatc
     6361 gactattttg atttaaagaa tgattttggt ggtgatctgg caaatgcatt tgtgaatgtt
     6421 aattcagatt acctgattat aagcttttca tctgactggc tatatccaac gtcgcagtca
     6481 cgggagattg tgcgggcact gcgtatgaac agtaaaaacg tagtgtttac tgaaattcag
     6541 agtgacaagg gacacgatac atttttattg cccaatgaac agcttgagca taatattgca
     6601 aactttttga agagggaatt tgaaaaggta cataaaaaat gaaagaatac cgtgtggcct
     6661 atgatctgat acttgattta atccctcctg gttcgcgagt acttgactta gggtgtggcg
     6721 atgggctttt attaaagtta ctgcaggaaa aaaagcaggt gaaaagcttt ggagtggaga
     6781 tttctccgga aggggtcagc cagtgcgtgg agaaagggct gtattgttat cagggtgaca
     6841 ttgatgaagg attgtcggac tataaagaca actcttttga ttatgttatt gttaatcaga
     6901 cattgcagag tacaaagaaa ccggatgttg ttatccgtga atcgctgagg attgcactca
     6961 atgcagtttt cagcttcccc aactttgctc attactcatt acggttatac ctgtcattgt
     7021 atggaaggat gccaaaaaac aaactgttgc cctatgaatg gtatgaaaca cctaatatac
     7081 accttttaac aataaaggac tttagagact actgcaggga tttcaaatat cccattaaaa
     7141 aagaaatgca tttttcaaca attcgttcgc gttcagttat cataccggta atgcctaatc
     7201 tttttgcaga atacggtttc tttatacttg atggacaacc ctttacttct gccacactgt
     7261 aaaatactaa gattaatgcc gcatggagta ttttcttgta tttttactat ggtatggcta
     7321 cattgtatgt aaactcttat aaaaccaaag gaaaactatg cctcaattta aggtggtatc
     7381 agcgtataaa ccttcaggtg accaggcaca agcaatagaa aaattagcag aaggaattgc
     7441 cagcggttat aaataccaga ctctgcttgg agttaccggt tcaggcaaaa catacaccat
     7501 ggcaaaggtg atagaaaaag tacagaaacc cacattggta cttacccata ataaaacact
     7561 tgcagctcag ctttaccgtg agtttaagga attttttccc aataatgctg ttgagtattt
     7621 tgtatcatat tatgattact accagcctga agcgtacgtt gtgtcacagg atctgtatat
     7681 agaaaaagat gcaagcatta atgatgagat agaccgccta cgtcttaaag ctacatcatc
     7741 actggttgaa cgggaggatg ttatcatagt ttcatccgta tcatgtatct atggcattgg
     7801 aaatcctgaa agctttgaaa aaaatcatat acggcttgcc gtgggggata tggttgaccg
     7861 ccaggagctt ttaatgaagc tggtgtcaat tcattatgac cgcaatgata tagcatttac
     7921 ccgcggtaca ttcagggtgc gcggtgatac tgttgaggta ttccctgcca acctgcagga
     7981 tgcgttgcgt atagtgtttt ttggtgatga gatagaagcg ctgtacaaaa ttaaccccgt
     8041 gactggagca cgccaggaaa cgcttgaaaa ggcatacatt tatccagcaa aacattatgt
     8101 ttccagcaaa gaagaaatgg aagaaaccat acgcatgatt gaaattgagt tagaacacag
     8161 gataaaggaa ttcatggatg caggcaaact tgttgaagca cagcggatag agatgcgtac
     8221 acgatatgat attgagatgc taaaaaccgt tggctattgt aatggcattg aaaattattc
     8281 acggattatt gatcgcagac agccaggcca gccaccgcat tgcctgcttc attattttaa
     8341 gagtaactat ctcatgttta ttgatgagtc ccatgtaaca ataccacagg tacggggtat
     8401 gtatgagggc gatagatccc ggaaagtgaa cttagttgag tatggtttca ggctgccatc
     8461 agcacttgat aacaggccat tgaaatttga tgaatttgaa gcaatgatag atcaggtagt
     8521 atttgtttca gcaacccctg ctgactatga gatagaaaaa tcagatcagg tagttgaaca
     8581 ggttatacga ccaacaggct taatagatcc tgacattgag gtcaggcctg caaccaatca
     8641 ggttgatgat ttaattggtg agattaaaaa acgcgttgct gccaatgaac gggtactggt
     8701 cacaacatta actaaaaaga tggctgaaga cctgaccgag tacctgacgg aagttggatt
     8761 gaaagtgcgc tatctccatt cagaaattga aacaattgag cgtgttgaaa taatacgcga
     8821 tttgcgaaaa ggagagtttg attgccttat tgggattaat cttttacgtg aaggacttga
     8881 tatacctgag gtgtcacttg ttgctatact tgatgcggat aaggaagggt ttttacgttc
     8941 aacgcgttcg ctcattcaga ctgcggggag ggctgcccgt aatattaatg gcaaggtaat
     9001 tatgtatgcc gacactgtta cccgttcaat gcaagaagca attgaagaaa ctaacagacg
     9061 acgtgcactc cagatggagt tcaataaaaa acataagatt actcccagga gcatttccaa
     9121 agagatagtt gacataattg aacgtgaata tcattctgac gatgtttttg ctgatatagt
     9181 tgcagaatat actcagagtt atcggactgg caatattgaa gaattgaaaa aggttcgaga
     9241 taaaataaag caggatatgc ttgaagcggc tgacaatctt gaatttgaaa aagcagcaat
     9301 acttcgtgat cagttatttg atattgagaa gaagattgga ctgtacagca aggtggcaca
     9361 gtgacagtaa tcagaggaat agtatatgga aataccttca tacgatgaac tatcgccact
     9421 tattgaaatg gcattgaaag aagacattgg tagtggagat attaccagta atgcaatttt
     9481 tgatgaatac cattttaccc gtgcacggat ttattccaag agtgaaggtg tcttttgcgg
     9541 aggatttttg attgaatata tataccatac catggaccct tcggtatcgg taacgctgta
     9601 ccgcaatgaa ggggcaccgg taaaggaaga tgatgttgtt gcggatattg aaggcaatgt
     9661 acggacgctt ctttcgggag agcgcacagt actcaatttt ttacagcgaa cctgcggaat
     9721 agccacacga actgccagaa tggtttcgat tgtccgtgat acaaatataa aaattcttga
     9781 tacccggaaa acattacctg gatttagact tcttgataaa tatgcagtat ggtgtggtgg
     9841 tggcaccaat caccgtatgg ggttatttga tatggttttg ataaaggata atcatattaa
     9901 ggcagcaggt ggaattttgc aggcagttga gatggttaaa aaggtgtacg ataacaagta
     9961 tgttattgaa gttgaggttg aatcgctgaa tcaggttcag gaagcagttg aagcaggggc
    10021 agatatcatc atgctggata atatgtcaaa agacagaatt gcattagcgc tatcaattat
    10081 caatgggaaa gcaaaagttg aagtatccgg caatatggat gaacataaat taaaagaatt
    10141 ttctgattta cccattgatt atgtgtcaat aggtgcacta acccattcag ttaaggcatt
    10201 tgacctttca atgcggttac tgtagcttaa taggaataga atcctcttcc agtcttttta
    10261 cccagatacc cagcctcaac atatttttta agaagagggc atggcctgta ttttggatcg
    10321 ccaaagcctt cgtataattc ttcaatgatt gccagcacta catcaagccc aatcaaatcc
    10381 gcaagctgta atggacccat tggcaaattc aggcctaact tcatggtttt gtcgatagtt
    10441 tctgcagaag cagctccttc atacagagta aagattgcct cattgatcat tggcataagg
    10501 attctgttca acacaaagcc aggataatct tttgattcta ctggtgtttt acctaatgat
    10561 tttacaaaac tttttgctga ttcaattgtt tcacgggtag tagcaatgcc accaactatc
    10621 tccaccagac gcattgcacg tgcaggattc ataaaatgaa tgcctatgac atattccggt
    10681 cgtttagttg ctgaagcaat gcttgtgatg ggcaatgatg atgtattact tgcaagtatt
    10741 gtgcctgctt tacatatagc atccagtgac ttgaaaacat tttttttaca ttcaaggttt
    10801 tctgggatgc attcaatgat aatatcagct ttatgaagat ccttaatatt tttatgcaat
    10861 tttaaaagcg aaattatctt gccccgttgt gattgctcca atcttttttc ttcaacatac
    10921 ctgtcaaggt gccctgttat tgtttcatat gcatgtttca attgtgattc atcaatgtca
    10981 tggagaatta cctcataatt gttttcaatg gctaaatgtg caatatcagt acccattatg
    11041 ccagcaccaa caattcctat taacatacat acctccatta tacagtttcg ttgactatat
    11101 ttctttttgt gccattcaag aacgctttaa tattttcagc aatctcgttt ataagtgcct
    11161 gcctggtttc ccgtgaagcc cagccaatat gtggcgttat aatacagttg ggtgctgaaa
    11221 gaataggttc attaatatca ggtggttcct gttcaagcac atccagagct gcaccggcaa
    11281 tcacgttatt atgaagtgcc tgtgcgagcg cctttgtatc aacaataggg cctcgtgcca
    11341 tgtttattat atatgctgat ggtttcatca tagcaagaaa atctttgttg accatatggt
    11401 gtgtggattc attgagcggc atgtggatag atataaaatc agccattgta caaaattctt
    11461 caaggggata tcgttcaact gaggtatcag tgtaggagtt ttcccttttt agtgcaataa
    11521 ccttcatgcc aaatgcctgt gccagttttg ccactcgttt cccaatgtca ccgtaaccca
    11581 tgatgcccag cactttatca ttgatgcaca tggtaggcca gttccccatg gtaaaaatgg
    11641 gtgagcggct ccagcttcca tcatgggtgg cagcattgta tttaataaga ttattagcaa
    11701 gggctaaaat aaaacacaga gtgagctgtg caaccgcttc gcgtgagtag ccgggaacat
    11761 tggcaacggc aatgttttta ttctttgctg ctttgatatc tatagtgttg tagccagttg
    11821 cagcaacagc tatgagtgag cacttatgca attgagctat ctcatcagca taaaagacaa
    11881 ccttgttggt aataataata tcggcatcct tgcttcgcga tatggtttcg tgtggctttg
    11941 tgacaggata ggtaatgaat gttcccagag atgcaatggg agtgaaatct atatccccaa
    12001 aatcaaccgt tgaagcatct aaaaatacaa ttgttttact catacggtat ttcctttcaa
    12061 ctgtatgtat tgcattatca tggacatgtc aatagtttat tacaggttaa tatacataat
    12121 tgtggttggt ttgccatcat tgtcaatggc ttcttcaagc acggaaaatc ctcgtttggt
    12181 ataatactgg ataagattga cagtatcatt gtgtccttca aaaagatata gtgttttgta
    12241 ccccaattct tttgttttag caattattgc tttgactatc ctgtcgccaa ttccctggaa
    12301 gcggtattcg ggaacaatat acagtgatgc aagccatgga ttgaggtcag tacgggacca
    12361 caggtcatta tttttcagtg aagccatgcc tacgggaagc attttttcat taatggcaac
    12421 ccatgccagg ggtaccgttg tattgcgagc gcgttctata tatgactgga ccagcgcagt
    12481 aaagggtata tcccttttac tgtaccattg attatatgac cagtatgcca gtaccggtgc
    12541 gtagttgggg aaattattaa gagggtgtat ttcaaccatt ggtgctacct cgtattattg
    12601 ttacgtagta ccagagatag caccaatagg tattgatttc aataattatt tggggagttg
    12661 aagccgttgt tgtacgcttt tgagttgtcg tttcaattct ttttcatccc atttaagttc
    12721 ttttgctgca gtttcagcta ccaaattgag tacatcattg ccaggatttc ctgcggtgca
    12781 gatacctgtt cgcctgaata cgatatctga aagagtgcgt gccatctcat ggcgtattgc
    12841 ataaataact tctgcaagaa tttccccatc atgagtaact atctccctgt acttcttgtt
    12901 tgaattagct atgctcatca atgcatctaa ttctgatccg taatatttta ccaggactgt
    12961 aagagtgttt ttattaaatg aaggatatga attgtatacc gtattaaaga atgcttcaag
    13021 gtcttttatt gtgcatcctg ctaaatattg tgtgtgggta actgaagggg ggagtttgcg
    13081 ctgtattttc ttttcaacaa gcttcatgac attaaccgcc aggttacggc tggtagtata
    13141 tttaccacct tcaacagtaa taaggccatt aacaccttct tcagcattat cgtatatttc
    13201 atatcgccgt gatgactggt atgttccttc tacctgatct tcaaccagcg gacgcaaccc
    13261 accataggca aatctaatgt ctttaaatgt gaggttgccg tcaccaaatg cactgtttac
    13321 atcatcaagc agttcctgta tactctgttt tgtgacggta tactcatcgg ggttgccaat
    13381 atattcctta tcagtggttc caatgagaga gtaccctctc cagggaataa ggaaaaaatg
    13441 ccttcctttt cgtggcataa gcccaacagc atatgtattt accataggtt ttgtgattat
    13501 gtgaattcct tctgatcgcc gtatttgatg ttttgtcttc ccattttctg cttttttcag
    13561 gattatatca gcccatggtc caccgcagtt gatgatgaga ttgccgttaa tggttacttc
    13621 cctgccagta agtttgtcat gaacgataac acctgtgatt gaattattgg tgtatacaaa
    13681 attcacaact tcagcatagt tagctacttt agcgccgtac tgtacggcag atttgataaa
    13741 tgctaaagtt aagcgttctg gaaatatgct ctgacaatca taatacacgg tagctgcagt
    13801 taaaccttca gggcgaatgt ttggttcttc ctgcagggct ttttctttac tcaatgtaaa
    13861 gtgcatagga attttttttg atttatccca ggtaaagcct ttatcatagg atagtatatc
    13921 atacagaatc attccaattt taagcaccca tcggttgttt tccagtctgt tataattggg
    13981 tatcatgaat gggaaaggat atacaaggtt tggtgcgata ttttccagta tccttctttc
    14041 tcgcaatgat tcacgcacaa gtccaaattc aaagtaattt aagtatcgca gacccccgtg
    14101 aataagtttt gaggttgctg cagaggttgc ccatccaaaa tcgtttttct caagaagtgc
    14161 aacagtaagt cctcgagatg cagcctcata ggcaacagca gcgccggtaa tgccacctcc
    14221 tacaatgata caatcaaatt gttctttagt attttcttca ataaaacgtt tcatagcatt
    14281 accccttatt gttatagatt tgcgaatagt atgcaaaaat tcttttttca gtgtattcca
    14341 gtacactatg tgttatagtg tatgcctttt tgctgttttt agctttaaag gcttccagaa
    14401 gttcacggtg aaatagttct gagcgcttct ggttatcagg attttcaaaa tacaggtagc
    14461 caaaatccct gaatatctgg ttgaagaaat ttaaaatgaa tatatacagc aaattcctgc
    14521 ttgctttagc aataagctga tgaacagcaa ggtctttttc caggatacta tcatgactgg
    14581 ttactatcgc ttcaaattgc tgtaattctt ctctggtgat gttttctgaa gcattggctg
    14641 ccatctgggg tacaataatt cttcgaattt caagaagatt ttgcagtatt gaaagaggaa
    14701 tagtatcatt aaggtatatc atcatcttga aaagttccag attgccactg gacaggtaat
    14761 ctttaatgaa gatgccttca ccgtggcgga tttcaataag ccccagtgtc tccagtttct
    14821 ttaatgcttc acgcacggta gccctgttga catctaaatc agcagcaata tcccgttcgg
    14881 atggcagttt atccccgggc tgcatctgat taatgatgcg ttttgcaagc agggaaacta
    14941 tctgctggtg aagggtgttt ttctgtattt ttcctaatgt attttttttc atagcaacac
    15001 ctttatagtg gttcagtggt tcaaccacta atagtatacc atgagtttat agtaaatcaa
    15061 aatatttata aaaaattttt attcaattcg gcgggttccg tcttcaatgc tgcattcgta
    15121 aatttggggg atcctgtttt ccctcttttt aaatttctgg gcgatagttc ttgtaaaatg
    15181 ttctaccgca tataatgcca ccatgcttat agtacatcca ccaaatcccc ctcccataag
    15241 ccgtgagcca tagactccat caatatcgct ggcccattct accatttcat ctataagagg
    15301 aatactgacc tcatagtaaa cgcttaagct ttgatgagat ttatagagaa gttcaccggc
    15361 ttttttaaga ttgtattgct gaatttcacg ggcaaattga tgaacacggt gattttcgta
    15421 tataacatgg taggtgcgtt tatattctgt ttgtggtaaa atattctgta tttgaattag
    15481 atcatcaaca ctgcaatcac agaggttttg aatggcaaaa cctgcttttt ttaacgttaa
    15541 tagagcccgc tcacattcgt gccttctatc attgtaggct gattcacgca agctgtgttt
    15601 aacaccactg ttgacaagaa taaacctgac agttgaggta tccacaggaa tatagctaaa
    15661 ttccatagtc tgggtattga gcattattgc attatctttc ttaccattga tgatggccat
    15721 ctgatccata atgccacatt taacacctgc aaaatcccat tcaaccttct gcgcttgcag
    15781 tgcaatgtcc ttactcttta ttgttatatt attgagcgaa tagagtgcat acaatgttcc
    15841 cacagtaata gaagctgagc ttgacatgcc agcaccaacg ggtagtgtgc tgtagaaact
    15901 aaagtcaagg ccacagtcaa attctatatt actgagtaca caggcgccat agggatatac
    15961 ccaccatgca tgtgctgcat gagcttttgt ttgcggagaa tggagattgt taatggccac
    16021 ttcacctgtt tctttatggt ttgcagaaac aaaccgtata atgccatcat tatttcggga
    16081 aatacatata tatatgtgtt tgtcgatagc agccggtaaa acgttaccac cattgtagtc
    16141 aatgtgttca ccaataatat tgattctgcc aggtgaagca aatagtgcac aatcagttga
    16201 tcgtgggaaa tactcacggt gatgggcgat aacggtatct ataattgagg acatggtgtt
    16261 tactgggata ttaaatgaac aaggttatat gcacttctgg ttaaagctat agtgacaggc
    16321 atgtccgcag cagtatgaac gtattcaata ataccaaaaa aggatagcca tgttataaca
    16381 gaaaaatcaa gaagtgatat ggcaaaaagg tcatagggat ttgcaattcc tgtaagttca
    16441 aaatatgtac gggctattga atctaactgt acaggtttat ttgaagaaag aatgatatcc
    16501 agtaaaagct gccggttatt tttgatatca tttgaaatat aaccaccaga gggaaacaga
    16561 tttttccagt tacatttatt ccagaatgct aaaaacagcc tgcgatagag ttctcctgta
    16621 ttgattgtgt caactgaatg ttcattagta attcctgctg cttttaacat aataactgtt
    16681 tgcttgcagt aaataacgag taaatcaggg tcatagtgag ccaagggtcc atccagaagc
    16741 atatccacgc ataaagaata aaaattttgg ggtaatggtt tattcttttc aggatacaca
    16801 tcaaaatgct tcattatctg gatgatacat tgagccagtg tgattgctgt tgtggcttct
    16861 attatttgcg tgtgttcgtc ctttacttct tttatgataa tggaattttc aaatggcagg
    16921 gttgaatccc atactaatct gtaacatgat tgaagaacat ctttcataat tttatctttt
    16981 ttacctgttt ttttcgttgc aaaccgtttt tcaatttcgc gagcaatcag gtcattggtg
    17041 agctcatttt caagtgcata cagtgccaga ttattaattg ttgatctgaa ccgatgtgat
    17101 ggcacatcag ctgaatgttt cattgtttgc tttaacccca ttaaaaattt tacgatatag
    17161 taactggcaa aagtatatga tatttaaatg cactgtgtga gcaactattg ctatgaaatt
    17221 aattgataaa cataatataa taaattaatt tttagtcaat taatatttta ataaaataaa
    17281 aaaaagttta gagaaaaaaa tactgcacat ttatttgctt tactaatatt aatataaaca
    17341 gtatgtatcc ttaataaatt tttggttaat aacacatata aactctctgt cttttgatta
    17401 aggtgtatag ttaaatatat actgaaaaat ttttttgaat ttgcttgcat taatatcttg
    17461 taattttgta ttactcatct cataaatgaa tagcatgttt acattgctac tatagataga
    17521 tatatcaaga ggtcgtggga tgtggttatt atcgttaata gatgcaacac ataatgatgt
    17581 aaaagagata caggaaaatt caaaggatat tcttcatttt ttggcagaag aaattggtga
    17641 gcgcacatta cggaaatatg ataatttaaa tcgtacgcgg cactatatcc atgattattt
    17701 aaagaaatat aatgacagag tttatgagga aaaatacctg gtgaatggca tggaagttgg
    17761 gaacgtcatt gctgagatcc ccggttttga agataatgat gagataatta tcattggtgc
    17821 acattatgat accattgaag atactcctgg ggcagatgat aacggttcgg caatagctgg
    17881 gttactggaa cttcacaggt tattatcgca atatagtttt agaaaaacat tgcgttttgt
    17941 agcatttaca ttagaggaac ctcctttttt ctcaggaccg tatatgggta gtatggtaca
    18001 tgctaaggga tgtaaggaaa gagacgaaca tattgatttg atgatttgcc tggaaatgat
    18061 tggctatgct ggtagcagga tacatcagga ttatcccttg agtagtatgc aaaaagaata
    18121 ccctccttat ggcaattttc tggctgttgc tgcattgcca agttatgctc cctatgctta
    18181 taaatggaaa cgggagtata acaggtttgc aaaaaataaa atatttgata tgattggccc
    18241 cgcttcaatt cctggtatta atctatccga ccattattct ttcaataagt atagttttcc
    18301 ggctataatg attaccgata ctgcttttta tagaaataaa aattatcata ctgagggaga
    18361 tacttacgag actattaatt ttaaatttct ggcagaagct atatttaata catttatggc
    18421 agtaaaaact atagcaaatg aaagggatat tcttgaatga gtgattttat tacaattcca
    18481 attcaggatt ctcatcgtct ggttaatgca ggtaatgtta ttttagtatc tgctcaatca
    18541 catgaaaaga aaaccgtaac cactgttgca tggcatatgc cggtgagcag tcagccaaag
    18601 ctattatcat gtgctcttgc aaataagcgt tatagccttg aattaattaa ggaatccaga
    18661 tgtttttgca taaatatccc tgaatttaaa ctgctggatg ctgttgtctt ttgtggtacc
    18721 tattcgggga ggaacgttga caaatgtaaa gaggcaaatt taaccgctgt gccatgttct
    18781 aagatacatg gatttttcat tgacgaatgt gttgcccata ttgaatgtga actatattca
    18841 atgattgaag caggggacca taccattgtg gttggtaacg ttactggtgc gatggcgcag
    18901 aaacatcttt ttactccgga tggagttatt gatattaata aagttcaatt aattcaccac
    18961 ctgggtggta ctcattttgg gatattaaaa tcacaggaaa cgtaatgcca tggaatgtaa
    19021 aatagaacga aatctgtcac tgtgtacctg cagttatgat ccctgttcac gaaaggggtt
    19081 atgctgcgat tgtattgcat accatctgaa aaagaggcag ctacccggtt gtgccttccc
    19141 taaagatgct gaaagaacct acgacagatc atttgagcac tttgcacggt tggtacaaac
    19201 aggcaaagta taatctattt cttcgccaga tagtgcaaag gatctaatgg cttaccagag
    19261 tggtttatct gaaagtgtag tgacgggttg ctatcgctta aaaatccaat tgttgtgcct
    19321 tttacaacga actggccttc cttgacagta attgcatcca gcttagagta gattgtataa
    19381 aagtcttttt tatgcatgat aaccacaaag ttaccaaatc ctcgcatagt tccaattttt
    19441 gctatagtgc cttcagcact tgcaacaacc ttgctttttg gcctgctggt aataataatt
    19501 cctatggggc ggactccacc atgatcacgt gtgtatgtca taacaaatgg tactggccag
    19561 ataaaagaaa agctgctgtc aaattttttt tgtggtattt ggtttggaga tgatttcttt
    19621 gatgaagaat tattaacaaa atcatttgaa ggaataaaaa ttttttgccc aacctttatt
    19681 gtggtgaagt tgttaatatt gttggcttcg cagatcgcat caacagtagt attgtactgt
    19741 tttgaaagtc ggtatagtgt ttctcctttt ttaatgatgt gccatgtgcc ttttgaagac
    19801 gattgtttac tattattgtt ttcgtttgac tgactattgt gataggaggt ttcgctttca
    19861 acctgaggtg ttgtataata cactggttgt gttgaatagt agtcattttt gatgtaataa
    19921 ttatcttcgt cggtgattag aggggtacta tcgtcttcgt tgtaattgaa ctgctggtat
    19981 gttttttggg tgtttttttt gtgtatatcc tggggttgac ggtatgcagg aggaacacat
    20041 cccatatgga atattaataa aactattata aaacgtatgt cattggcact catccatgaa
    20101 accatggaac atctccttat gttccgttta aacgcacatt taccagaaag ggtataaata
    20161 cccttatcat agtatcggaa gaatgggctg gatagttaag caattaattt tttattcatg
    20221 ataatcaaca gtggtggtga tagttaattt gccatggcgt acgtttttta tggccagtaa
    20281 attatctgca atctttttaa cctgtgacga tgtgccccgt aacaataaca cctcaaggca
    20341 attatgttca tctaaatgaa catggactga tgatattatg tatttgtaat tatcatgctg
    20401 aaggtcattc agtttatcgg caatctcacg aacatcatgg ctgtatacaa gagtcaaggt
    20461 gcctattact tctgcatttg ggtcagtgat ttcttcttta accagcatat ctcgaataag
    20521 gtcacgtatg gcttcagaac ggttgacgta tcccttttcg ttaatcaagg catcaaacct
    20581 gtccagcagc ttctcatcta tagacacgcc aaaacgaatt acattattca tgctctgacc
    20641 tcatctggta tatagtataa atcaatagtg ttgtagcttt ttgcaatatg caagtaaatt
    20701 ttttatcttt ttctgcagta taaacaatat caagtattga aattgttatg ataggtataa
    20761 cattatatat aatagataac aggtttgata cgtgggaatg aaaataatag aaaaattgaa
    20821 ttgacaaaat taacattggt tgtacaatca atcaactata atgattttat ttttaaagga
    20881 ggcagttata gataaacata atatatagta taaattaaaa ccgatatatt cttattatac
    20941 ttctcccata catgtccaca ttcagcactc aaacataaaa ttataaataa ctaaaatgga
    21001 ggttagccat gcctgttttt tcaaacactg aaaaaatgta tgaagtattg ggtgagctgt
    21061 ttaatgcatt attagatgat acaacagcca gggaaaaatt tttagaatca gacatcatta
    21121 ttaagtttga tattcatgat cctgcagggc agatatggat atcaaatgaa gatggtggca
    21181 aggttatatg tggctcaaca cagttaaaac caacaatcga aatgacgctt tcaggtgatt
    21241 catgccacaa attctggcta caggaactaa caatgcctgt tgcattggcc aaagggttaa
    21301 ttaaggcaaa aggacccatg ccaaaagtta ttaaactcct tccacttctt aaacctgcat
    21361 atgcagcata tcctgaaatt gctaaaaaac atgggctgat ataaaggggg taacagatga
    21421 gtattgaacg aagcatgaag caatttgagg aagatagaaa agcagccgtt aagattgctg
    21481 cacacttatt actggcaagt gcaaccacca caccacgtgt aggaggtgta ggtgagtgta
    21541 ctgttcacat aattgatgat gaatgtaaaa ttgaggattt atgccaggca atagaaaaga
    21601 tggcaaagga aaatgaacgc tgggaatttt ttacccgtga tgccatgatg ctcagagatg
    21661 cagatgctgt gttgattatt acatcgctac gatgttatac cgatcctgca gatactaact
    21721 gcaatatgtg tgggaaactc acctgtgaat atttaaaaga aagtgaaaaa ttgcctgatg
    21781 atccgggtat tgcctataaa ggtccgttat gtatattcag agctaataat attgcttatg
    21841 ctgttgatgg gatagtgtca ctggctcgga atttaggcat tgattatgga gtgttctggt
    21901 cagcgggtgc agcagctatg cgtatgggta tagtgccaaa gaatacgggg ttttgtttgg
    21961 ctgttggtat ttcagtaaca gaaaagagcc cattcaggga tatcccgttg cgatatgatg
    22021 agataaatga aaaaactatg aatgacagga tcatcaagcg attgtggcct cagttcagat
    22081 ctatttacag ttaataatgc accacaactt cattgatttt gcccataaca gattgcatca
    22141 tgtaatatat aacaaagttt atggaggttt ttaatgccag caatagaaat gaatgagctt
    22201 atacagaatg gattaaacag ggcagttgag cttatggctt tagctgcata taattctttt
    22261 agatttggca aacgcaatac cataaaaatg gtgagccttt ccattgatga tttaaaggag
    22321 atagctgaat tctgctattc gttgggtgac atgtcaccac tggctgcgcg ggatggaagg
    22381 ttccttatgg aactggtaaa agagccatgc gcagtgcttt taattggcga taagcgaaaa
    22441 tcggatttta attacaattg cggagcctgt gggtacagaa cctgtgctga actaaataaa
    22501 gctgaagagg ttgaagcatt aaccgcgcgt gggccaagct gtcagtttaa aaatttaaat
    22561 attaatatag ctgctggtgc tgctgcttca atggcatggc gtttaggctt acattgcagg
    22621 gtattcagta cgatagcgtt tgcggccttt gcgctcaata aactggaagg tattgatatt
    22681 gcaacgtcag ttgcagtgag cgctgcaaaa gttgatcctt tctttgaccg gcatcagtac
    22741 tggacaaaag aacactggga tgaaattttt gcaaaagaat tccccacatt tacaaggggg
    22801 tttataggag ctatcgaaga ataaaataat ggaggggagt atggcaacaa aagcacatct
    22861 taatccagtt atagcttcgc acctggttga aattaaagca gatgaaattc ccgataccct
    22921 ggtttatatt tttgaacagg gtgatgcggg cgatgagcgc tgtacatata aagaccttta
    22981 tgaaaattca aataaaatag cacggttatt ccttgataac ggaatagata aaggcgatac
    23041 ttttgctgtc tttatgaaaa attatccaga atttgtctat gcgctgctag caggaactac
    23101 gataggagct atcatggtgc ctattgatcc gcgcagcagg ggtgacaagc tgaggttcct
    23161 tttacacaat tgtaatgcca aagcagtgat agtagaagat gaactattaa gcagtcttga
    23221 agaggtattg cccaatctgc cagcaataaa attagttagc gttgtatata aaaaagggca
    23281 tgctatacca gttgctgata aataccatgc attaaatgaa acaattgaaa agactacctg
    23341 ggatacagtt gatcagatga taatggatgt acggcaccca atgcagatta tatatacctc
    23401 gggtactacc ggtgacccaa aaggagtagc aatcaggaat aacagaactg gattgtttaa
    23461 tatcttaaac agtattgtgt ggcgctatca aaaagatgac atcctgtata atgggttatc
    23521 actgacacat ggtaatgcac aggcagttac tctatttcca gcactctcta tgggaataac
    23581 tgcagtattc agcccccgat ttaccaaaag cagaatatgg gatatatgta gaaaatatgg
    23641 atgcacgtca ttttcgcttt taggcgggat gatggcagga atttataatg agcccgaaaa
    23701 accggatgat gctgacaacc ctgtgaaagt ggtcataagt gccgggactc cgacaacaat
    23761 ttgggaagca tttgaaaaac gcttcaatgt taaaatactt gaatggtatg ggtcggttga
    23821 aggtggcttt gcatataaac ctatagggaa agggcccatt ggatcttttg gcaaaccagt
    23881 accaggtgta atggaattta agatagtgga tgaaaatgac aatgaagttc cggtgggaca
    23941 aaccggggag ttaatatgca gattattgaa aggtgaaaca aaagttgatt atctgggcaa
    24001 gcctgaagaa tcaaaagaaa agacgcgtgg tggttggttg agaaccggtg atatggtaca
    24061 cagggatgcc gaaggctggt attattttga ttatcgtaaa gggtcagagc ttagaaggtc
    24121 cggagatttc attcagccag aatatattga aacagtcatt ggcaaacacc ccgatgtatc
    24181 agaagtgtcg gtatatggtg ttcctgctgc ttccggtgct cctggggaaa gcgatcttgt
    24241 tgctgctatt gcctgctttg aaggaaaatc agttgatcca aagtcatttt atgagtatct
    24301 gaaaaaaaac ctggaagcaa attttattcc ttcatatctg cagattgttc cagagattcc
    24361 caaaacaata acagaaaaag ctttaacacg ggttcttaaa gaagactttt taaaaggcct
    24421 tgggacaata tataaatatg aagattataa ataataatcg tatagggaaa acatctactt
    24481 atggaggttc gtatgttgta tacagaactc aatatagaac taacagatga acatcagcat
    24541 cttaaagagc aggttcacag gttttcaatg gaagtattac gacctgcttc tctggaattg
    24601 gataaacttc cggctgaaga agttgtcaaa aaaggttcaa tttactggga ttgcatggct
    24661 aagatgtacc agaatggcta tcatactgtt ttgatttctg atgaatatgg tggattggga
    24721 ctggatccat tggcagttca tatcttctgg gaagagctgg catacggcag tgtgggattt
    24781 gcagtatcgt taggctgtag ctgctttgct gcattttatg ctaccatgct ggctgaggat
    24841 aagcttatag aaaaattcgt tatcccgttt gtaaattgta aggatgctag cgttatgagt
    24901 tgctggggaa ttactgagcc tgatcatgga agtgataatt tagcaccacg gacgagtttt
    24961 ttcaaagatg cctcattgac acaacaggtc aaggcgcata aaaaaaatgg tgaatggatc
    25021 atttcaggac agaagtcagc atggatttca aatggacctg ttgcttcaaa ctgtgcatta
    25081 tttctgaacc tggacccttc aaaaggtttg tctggaggtg gtatatgcct tattgattta
    25141 aatcaggatg gggttacacg gggcaaacca cttgataaga tggggcagag ggaacttcct
    25201 cagggcgaga tattttttga taatgttgtc tgccctgaag agtacatgat cgtagatcca
    25261 gaaagttatg aagccatgac ggaattaaca ttggggcatg ctaatgcaac aatgggtgcc
    25321 tattttactg gccttgctca agcagcctat gatatgacat tacaatattg tactgagcgt
    25381 atacagggag gaaaacgatt atgtgaccat caatgggtgc agaaaaaatt attcgatatg
    25441 tttgtaaaag ttgagactgc cagagcattt tcccgcgctg ccatgatata caatatgaat
    25501 accacaccac ctgatgttaa atattccatt gcctccaaag tatactgtac cgaagcagca
    25561 tttcaggtaa ccagtgatgc catccagctt tttggcggct atgggctgtc aaaagaattc
    25621 cccattgaaa aattgttcag ggatgctcgt gcagggatga ttgaggatgg ctcaaatgac
    25681 acgttatcaa tgacagcagg aaatattatt attaaggaga aattgggaga tgagtaatgt
    25741 tcacataatt ggctcaggga tgatacggtt taacaagtat cctgatgaaa cagtcaggtc
    25801 aatggctgaa aaggctataa agcttgcact ggaagatgct ggcatagata aaaaagacat
    25861 tcaggcagga tttttttcaa atacattctg gggcatgttt tcaggacagc attccatacg
    25921 aggacaggta attttcaggg gaatgggaat tgataaaatt cctgtagtta atgtagagaa
    25981 tgcgtgtgca ggtggatcat cagcattgca tttagcttat actggcataa aagcaggcat
    26041 gtatgatgtg gcaatagctg ttggttcaga aaaaattacc aatcctaata aggcactgtc
    26101 cctgggtgcc tatgcatcgt gtatggatgt tgaaaatttt gagaagcata tcaatatgat
    26161 aatcgaagta agtaaatcat ttaaaattac catacctcac gatgaaagca aaccaggtga
    26221 aggccgtagc gtatttatgg atgcatatgc tgttggagcg cggtggcata tggacaggtt
    26281 tgggtccact caacaccagt tagcagttat atgctctaag aatcatttac atggatcact
    26341 caatcctctg tcacagtatc aacacaaaat gaccgtagaa gaagtactgg cagacaagct
    26401 ggttgcctgg ccactgactc gtgctatgtg tgcaccggtt ggtgatggtg cggctgctac
    26461 aattgtatgt tctgagaaat ttttaaaaaa attaacagga gcgcgtccgg taaaaattat
    26521 agcatcggtt atgggacagg gtgaggatag ggaccttgat gcgccggata taggtgaacg
    26581 tttatcaagg ttagcgtatg agcaggctgg tgttggtcct ggcgatataa gccttgctga
    26641 actgcatgat gcaacgagct ggggtgagtt gcatcagact gaatcaatgg gtttttgccc
    26701 tgttggtgaa ggcggtccct ttgctgaaag tggcgcaaca acgttgggtg gtaaaaagcc
    26761 cattaacacc agtggtggac tggagtgcag aggacatccc ataggcgcat caggccttgc
    26821 ccagattcat gaacttgtac tgcaattgcg cggtgaggca ggagcgcgtc aggttgaaaa
    26881 cgcccggtta gggctggccg aaaatggtgg gggcaacata ggcgttgaag aagcagctat
    26941 gtgtattcac atcttagaaa aggtgcgata gttactgata atgtaatcaa tggctattaa
    27001 tttcaacaac aggtgggtgg ttatcacagg agcctcttcc ggtataggta aggcactggc
    27061 ggaatgcttt gccagggaag gggctcatct tatattaggt tctcttcctg gtgaaaaaca
    27121 catacttgaa agcatcaggg ataatcttca aaagcaacat gctattcaaa tagttactat
    27181 ctatcaggat tttgcagatc cggatggagc cgtaaaattt tatgatcaat gtaaaaagct
    27241 acaaaagcag atttactgtt tagttaataa tgccggtata atggcctatg gctatttcca
    27301 tgaactatcg cacaaacagc aaaaaacatt aatcaatatt aaccttatgg catatttttc
    27361 cctcatgcga ctatttattg aagacatgat acaacaagag gaaggggtta ttattaatat
    27421 atcatctgtg tcagcattcc agccgactcc gcaccatgct gtatatggtg ctgttaaagc
    27481 gtttgtgcag agtttaagcg aagctgtcga tcaggaatgt agaggtaccg gtatcagcgt
    27541 gcatacaata aatccatcct atacggatac gcccttgctt aaaaaaaata attttccgaa
    27601 taaaatctgg tggtaccgta tttcagggct ggcaaaacct gaagatattg ctcgcaaagc
    27661 aatcaaggca gtaaagcaag gtaaaagatt atatgtgccc ggggtcatga atgcattgat
    27721 tcatcttttc ctgccgcgat tgctgccacg aaaactgtcc aatgcaatag cacatattgt
    27781 tttacggcag gaaagataat cctgtcttta ttcatttgcc aggcctctgc ggaaaagact
    27841 gatatacatg agtacctgat gtgttatgct gttgatgatg gtaaacagtg atgtgtacag
    27901 atacccaacc tggaacagca tgatgagcgg aatggatata tatagatttt tatcaaatgc
    27961 aaaatacacc gccaacgtca tatagagccc caacacaaac tcaataatag agacaaaatt
    28021 gattgtatgc aggctatatt tgttttttaa tagcgttttc acgtggtcta ttgcattaat
    28081 acgattgaga gccaccacat tgtattttgg agtcctgtta aaatcactct ttttatcaag
    28141 cagtgcctct atgaccgctt tactgttatt aatggataaa ccaataccca gtgaaaggac
    28201 catgggcagg tacagaagcg gtttaatctt tgaatcgtaa atgtttttat tctggtaaag
    28261 aacaatgagc tctgtaaaat agtagaaaat cattactgat agtgtggcaa ggagaaatat
    28321 tggcaggtca agataggcaa actttttaaa ccctccgtaa taacgaaccc ataaagctac
    28381 aggcataaga agagtaagaa aaaccatgag aagatatgca aagttagccc ctaaatgcat
    28441 gaacgattca agcttaactt taaggggcag gtgtgaatgc aggatagttt tatataattt
    28501 tttaaatacc tgaatggatc cctttgccca tctgaattgc tgagccttaa atgctgaaat
    28561 ctcaatggga agttctgcag gtacggtaac atcgggaaga tataaaaact tccatccttt
    28621 catctgtgat cggtagctta agtcaagatc ctcggttatg gtatcatgtt gccatccacc
    28681 accatcaatg attgcctgcc ttcgccagat gccagcagtg ccattgaaat taaagaagcg
    28741 gcctgagcga ttgcgtgcgg tatgctctat catgaagtgg ccatctaaca gtatgctctg
    28801 acattcagta agcaaagaat aatggcgatt tatatgatcc catcgtgtct gcaccattcc
    28861 aatttcaggg tcggtaaagt aatggattgt tttttgcaaa aagtcaggat tgggcaaaaa
    28921 atctgcatca aagactgcta taaattcgct atcggtaagc ccaaggccgt tttccagagc
    28981 acctgcttta aaccctgttc tgtccgttcg atgaatatat ttgatattga atccttcctt
    29041 gcgtttgagt ttgcatattc gctcggcaat aattgaggtt tcgtcggttg agtcatcaag
    29101 tacctggatt tcaagcttct tttttgggta atctaattgc gttatagtat tgataaggcg
    29161 ctcaacaaca tactgttcat tgaaaatagg aagctgaaca gttactttgg ggagagattt
    29221 aaacattgat ttaggttgaa ttttcttttt ggcgtatttt agaaaaagat acaatgtata
    29281 gtatctatga gcaccaaaaa ttgataaaag taaaagtatt aatccataaa aaataagaat
    29341 aaacagcttc atagagtata tcttcctttt attattaaaa tatctttcag tatggttgat
    29401 gtgatatttt ttcagattta caatattgaa aatggattat accacagaaa aaaatatttg
    29461 caaaaattaa tgaatggcat actataccat gtcatttgta tgtcaatatt aattacagga
    29521 ggtttgtatg acacaggcaa ctgttcaggc gttacagggt ttacgtgatt tatccatggt
    29581 caaatggtat atcattccgc tactggctat tgtattttac atttatacaa aagaaatcaa
    29641 ggaagcacgt aaaactggcg actgggatgc agtatttgcg ggcgctacta tatttggcgt
    29701 ggattttttt aatgaaacgt ggaatggatg ggtgatgtat ctgtcacagc gatctgcagt
    29761 atggacaacc cctggcgata cggcactacg caccatggtt ggatggaata ttgagataat
    29821 gtttatgttt acattgttag ggattatctt ttatcacacg ctcagtggaa aaaatagtaa
    29881 aatccttggc atccctgaaa agtggttctg ggctgtagtg tatgcagcat tttgcgtatt
    29941 tgttgagtgc ctgcttaata ttggtggcca tttagtgtgg gaatacgaat actggaatct
    30001 ttctttcaag ggtgtgtggc ttatcttttt gtttgggtat ttccatttct ttgtctttgc
    30061 tattatcgtg attgggcttt caacaattaa aaagaagatt attgcagtat catgcatcta
    30121 tgcagtacct atcatcatga atatagtagc actgggaata ttgggctgga attattgaaa
    30181 cgaaggtgga atgtcgcttt gtttattgat gttataaaaa agttctgaag ggtcttcggc
    30241 ataaaagatg agttcatcat ccatgatata gcgcatcctt acctgttcaa aaaggaagct
    30301 gattttgtat tttttttctt tgataagttt ttcaatatgg ggaatgcatt gttttgagta
    30361 cactgcatgg agtggctcag tattccctgc ccagcagggg atgatgcagt catacatatg
    30421 ggggatttgc agcataaaag caataaattc ctgatttaag aaaggcatat cagctgcgca
    30481 tacaaaaatg taatccgttg ctgaacactt gagtgcagta tagatgccac caagcggacc
    30541 aagaccaggt attatatcag gatatacagg tattgtgaga tagttatatt tatcagtatc
    30601 gtttgcaata attgaaattg tttgaaacag cggtttgagt gaatgtaaaa catgttcaat
    30661 cagtggcctg cctttgaaca ggtgtaaggt tttgtcgcta ccaaaacggc tgcttttacc
    30721 gcctgcaagg atacatgctt ctatagcttc catagattac tggcgtgtat atcccatctg
    30781 tagtgctgaa gcatcaagaa aattggtaat gagattttct accgtcaggt caaactgtga
    30841 ttcatcatgt tgtttggtta tgcggattgt ttttatatat gtgttgcatt tgttgcagta
    30901 atcaaagcgg tatttttcat cgggttcata actgtaataa ccaagtgtat cggtgttttc
    30961 atttccacac gcaacacatt taacgcgttt gtattcccat tcgcattcac acagggcaca
    31021 gtgaaggaat cgtttgttgt tttttggctc aacaatttta gccatatcgg gtaaaaatcc
    31081 acaaaaggga cacaagcctt cactataatc agggaattgg gttatatcct gcaatgctgc
    31141 atctttaaga gcaatgaata atggcttgta tgcatttact gtcacaaata tcagctcatc
    31201 agtatcaagt ttattattat gagctattgc ctcaaatgta ctgtcttttg ataataatgc
    31261 tgtgattatg tctttgtata gctcaggatt gtcatcaagg tttgcaagca atgtatcaaa
    31321 tgagtaagcg gtttgatact gtgtaataag cgcagctata tttttgaaca ttttctttat
    31381 gccagcaatt atttcatcag tgaactgtag tgtgtgaggc tgaatcattg ggtactgtgt
    31441 ggtatcaaca atgagttttg aatcagtaat gattgaggca aggtgttgaa ggctttgaaa
    31501 ctgtaatgaa aaaagtgacc gatatagagc aaaaaggtct tctgaaaaca tagaattatt
    31561 caaaagcgat tctttacgct gttcaagatg ttctgtatga tgtatcattg gcataataaa
    31621 ttttccgctg ttttcctgca attgaagcta tagtcctgat ggtgacgtat ttgtcaatac
    31681 tttacaatga tttcttatac agttttttat agtaacatac gcaataatgt ttgacaatta
    31741 tatattaatt ttttttatat ataagatctc ttcgaaccct gttttctaag gctcatataa
    31801 cgctatgaat gcagggtcga aagggtaaaa agagaaatct ttttgcctcc catgcctgga
    31861 aagaagaggc agataccctt tttaagtaaa aagggagggg tctgtttata ctatttattg
    31921 tattatatgg gaggctacga tcacgccaaa atttaataca atagtgttat tgtttatcag
    31981 gcagaacttt atagtgctgc atgttttgtg aagaactact gtttattttt tcaggaggta
    32041 ctcatgccgg ttaccagaag agatttgttt aaattaacag gagtcaccgc agcgggttca
    32101 tttttgggtg gagtaacctt cttaacagga tgtaaatcca ctgacaagtt aagaggggca
    32161 aaggaatcca ccactatatg cccgttctgt ggtgttggtt gcggtattat tgtatcatcg
    32221 cgtgatggca aagtaataaa tgccgaaggc gatccagatc atccaattaa cgaaggtgct
    32281 ctttgttcaa agggaatgtc gttataccag atagcagtaa atccggtaaa acggcgtctt
    32341 gataaagttt actatcgtaa accttttagt gaagagtggg aaacgttaag ctgggatgat
    32401 gctatcaaaa tgatagctca gcgagtaaaa gaaacccgtg atagaacatt tctagagaag
    32461 gaaaatggca ttacagtaaa ccgttgtgat ggcttagcag gatttggtgg tgcagcactg
    32521 gataatgagg agtgctatgc atggtcaaag tgtgctcgtc tgctgggaat aacacggtta
    32581 gaacatcagg cacggctttg acacagctca actgtcgcca gtttggcggc ttcatttggc
    32641 cgtggtgcaa tgacaaatca ctggattgat attcagcatg ccgatgttgt catgatcatt
    32701 ggcagcaatg ctgctgaaaa ccatcccata tcatttaagt gggttcagcg ggcacgtgat
    32761 aaaggtggca agcttatcag tgttgatcca cggtttaccc gtacatcttc actggctgac
    32821 ctgtatgcac caatacgtcc cggtactgat attgtattta tcaacggtat cattaactat
    32881 gcattgcaga atgatcttat ccagaaagaa tatgtggtta agtataccaa tgcagcatat
    32941 ctgatagatc ccggttttgc ttttaatgat ggaatcttca gtggatataa tcctgctgaa
    33001 cgcaaatatg acaaaaagac atggaaatat cagcttgatg ctgatggcat acccttaaag
    33061 gatgaatcat tgcagaatcc tcagtgtgta taccagcttt tgaagaaaca catgtcacgc
    33121 tatacacctg aaaaggtttc tgcagctaca ggctgtccag ttgaca
//
LOCUS       DAORYL010000026        33070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1103_length_33070_cov_12.924489, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000026 DAORYL010000000
VERSION     DAORYL010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 33070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33070
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1103_length_33070_cov_12.924489"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /gene="ispF"
                     /locus_tag="PLV81_06715"
     CDS             complement(<1..465)
                     /gene="ispF"
                     /locus_tag="PLV81_06715"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOM09828.1"
                     /translation="MHNLTAYRTGIGFDAHRLVQGRKLIIGGVHIPYEYGLLGHSDAD
                     VLTHAIIDALLGASNLGDIGTLYPDTSSQFKDKYSIDMLKDTYQLLTKHNIEIINIDA
                     VILCQKPRIAPYSEQMKEILSAACGNLPVSSISIKGKTTEGMGFTGTGEGIAA"
     gene            complement(498..797)
                     /locus_tag="PLV81_06720"
     CDS             complement(498..797)
                     /locus_tag="PLV81_06720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOM09829.1"
                     /translation="MEINLNNKGNLVEVKVKGDIEMMSIKEFKEKLLAVGEDNDVDIA
                     LDLSEVDYIDSSGVGVLISLNKIQKKKGKKLELINVSPKVLNVLKLSSLAEVFNL"
     gene            1043..2920
                     /locus_tag="PLV81_06725"
     CDS             1043..2920
                     /locus_tag="PLV81_06725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOM09830.1"
                     /translation="MQLKKTYTDVYESKKIDFQKLCEIIKPGSRVFLSSGPAMPVYTV
                     EQMMTSDAPTLQDLEIIHLITLGNYLKTDSNLYKFRLKTFNTGESISKDIEQGKADFV
                     PAHLVEIPFIFSSGAIGVDVAIIQVSPPDHRGFMSLGIAIDVANIVIENAKTVVAEVN
                     PYVPVTMGDTAVHIDAIDYIIESKVPLIEREYKRYDEIMDKIGWHVANLIDDGSVIAL
                     HVGRQFDAIAKHLHNKRELKVYSHVVSDWIIDLIESGAVKIDRGIRNLAPVTLSYCYG
                     TRMLYDYVDRNPMFEFLPLMRLLYPTVVQRIPKLVSVMNVKKIDLSGESVVFYSGDNL
                     LSGYESKLNFAVGAAFSRGGKAIVALRSIDQHGDSNIVIRHTETEQVRSTLGVTRYVV
                     TEYGVANLFGKSIRERALALIDIAHPNHRLSLLNQAKEAGLVYPDQIYVIENIINYPV
                     TLETMKTFKDGLLVKFRPIKPSDEDMMRRLFYQFSDESKYLRYFARIRTMPHKQMQSY
                     VNIDYDRILSIVGIIQHAGTERIIAEARYGYDDNEKTYEMAFIVDEEFQGKGIATFMA
                     RYLMKIAKERNIEKMSANVLPQNDKMMKVFEKCGVPFTQRFEDGVMHFDFHLQKADIV
                     I"
     gene            complement(2913..3554)
                     /gene="thyX"
                     /locus_tag="PLV81_06730"
     CDS             complement(2913..3554)
                     /gene="thyX"
                     /locus_tag="PLV81_06730"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOM09831.1"
                     /translation="MEIYSPKVTLLAITPNAEKLIEEAGRTCYLSLDRITEGSETNFI
                     RRCIMNGHHSILEHASASFRVLGASRAFTHQMVRHRLASFSQQSQRYVNEEEFNFIIP
                     PSIAANNEAKTIFTDFMEQARIIYKKLRELSIKKEDARFVLPNALESQIVISANFREW
                     RHIFSLRLEKAAQWEIRSVCMQMLKILQDEAPSVFGDFIIDEATCTAKSNLIK"
     gene            complement(3569..4171)
                     /gene="rsxA"
                     /locus_tag="PLV81_06735"
     CDS             complement(3569..4171)
                     /gene="rsxA"
                     /locus_tag="PLV81_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009424153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOM09832.1"
                     /translation="MEIKILFTILISTIFINNYVLSRILGLCPYLGVSKKLDSAIGMG
                     LAVIFVMTMASFFTFIIYKFVLIPFHIEYLRTIAFIIVIASLVQLVEMVIEKISPALY
                     QALGIFLPLITTNCAVLGVAVLNIESGFITQEFGLLNSVVQGFGAGVGFTLALLLMAG
                     IRERLEIAMVPENLKGLPITFITAGLMAMAFLGFSGMKIF"
     gene            complement(4196..4804)
                     /locus_tag="PLV81_06740"
     CDS             complement(4196..4804)
                     /locus_tag="PLV81_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOM09833.1"
                     /translation="MDYFKEFFKGVWQKNPILVLGIGMCPTLAVTTSASNAIWMTIAT
                     TFVLLGSNILVSAIRTIVPSHVRIPIFITIIASFVIIVELTLKAFQPDVYKALGIFIP
                     LIVVNCIILGRAEEFASKNSVLSSILDALGMGLGFGLTLFILATIREVLGANKLFGYV
                     LIPGMKPAILMILAPGAFFTLGLLLWAMNVIKSRKALFKRTE"
     gene            complement(4804..5481)
                     /locus_tag="PLV81_06745"
     CDS             complement(4804..5481)
                     /locus_tag="PLV81_06745"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOM09834.1"
                     /translation="MKPADIVKATIIISVIGFISAFSLSFMKKITQEPIAKQEQEKKL
                     AALALVLPGYTVQQEKTVTIDDSPFTYWIATKEENRIVKKAWAFLAEKKGYGGPVQSI
                     VGVDESNTILGFTILSQNETPGLGARCVEVASNETFVDHFITKNLTSKEEVSWFQEQF
                     IGLNASKPIAIHKKGDWHKEMKDELIQHNAVTAITGATITTKAVTQSIEEGIQKLHKA
                     LTLQEVQ"
     gene            complement(5820..6905)
                     /locus_tag="PLV81_06750"
     CDS             complement(5820..6905)
                     /locus_tag="PLV81_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOM09835.1"
                     /translation="MEIKDADKKRLLEDLVLSHAPHIRDTQSVPLVMWLVVLALLPAT
                     VFSCYIYGLYVALVIAVSIISALISESIMQYLLKKPITINDGSAVITGLLLAMNLPPH
                     VPLWIPAIGSAFAIIIAKQLFGGLGYNIFNPALAGRAFLLASWPVEMTTLWHKFPGGH
                     VITQNPIFDPSMPQQLVDSISGATPLGALKEGPKVLHELSLQTQPLYDFLFSKAMIKS
                     LFIGNIGGCIGETSALLLLIGALLLLWKRIITWHIPVSYIATVALIMGIYYYFQGIPN
                     LQYAILFHVLSGGLILGAFFMATDMVTSPVTKTGMIIFGIGCGILASVIRLWGGYPEG
                     VSYSILLMNAVTPLIDRLTRPKVFGTR"
     gene            complement(6930..8249)
                     /gene="rsxC"
                     /locus_tag="PLV81_06755"
     CDS             complement(6930..8249)
                     /gene="rsxC"
                     /locus_tag="PLV81_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOM09836.1"
                     /translation="MMIHSFHGGIHPPENKHFTNRIPFKYLSVPQKCYIPLQQHIGKP
                     ATPVVEVGQFVEEGQLIGQANGLISANVHATVPGKVVEIAEYHTPYSEHGLCVVIEAE
                     GSFSTSGKVREKNDMTALDRDAILEKVKAAGIVGLGGAAFPTAVKLNPPANRTIDTLI
                     INGSECEPYLTVDDMLMQTHPEEIIEGIQISMKILGVSKAFIGVEKNKALAIKALQTA
                     VTSIKSEDISVVPLRTRYPQGAEKQLIYTITGREVPSGGLPMDVNVVVQNVGTIYAIR
                     EAVLFDKPLIERYITVTGKIVQKPGNYKVRFGTRVIDILEECGGLTENPAKIIMGGPM
                     CGLALNHMDIPIVKGTSGILFLAKDEVEVDSFRPCIRCGRCVAVCPMNLMPCDLGNAV
                     EKQRYDIAEKLHPFDCIMCGSCSYVCPSKRPLSHFIKVVQTVLSAKR"
     gene            complement(8253..8531)
                     /locus_tag="PLV81_06760"
     CDS             complement(8253..8531)
                     /locus_tag="PLV81_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09837.1"
                     /translation="MPKEKCFCANCMNCIVIRQYESDPERFVLRVRCTKKKWTKRSGE
                     EKLYKYFTVSRRTVDNCQYYTEAGDLYPYIKNLRKDLPIKDEIYSTRE"
     gene            8878..9330
                     /locus_tag="PLV81_06765"
     CDS             8878..9330
                     /locus_tag="PLV81_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="HOM09838.1"
                     /translation="MGYAVRIYEAFKEDEVKAKVLAEFIEKVEEAINNNQIATRQDLN
                     VQELKLTKEIKEVELRLTKEIERVRADLTKEIEQVRADLTKEIEQVRADLTKEIEQVR
                     GELRLEIEKVRVELHQVKASILKWLIGLLLAQTVTIIGVIVGLFQAMK"
     gene            9998..12469
                     /locus_tag="PLV81_06770"
     CDS             9998..12469
                     /locus_tag="PLV81_06770"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOM09839.1"
                     /translation="MVKNLSRIIILIMMPALISQCSIMGHYFKSSEKQYSGTIHIEGL
                     KQPVEVRYDNLGIPHIKAHTEEDLFLATGYVMASYRLFQMVMIKMAIQGRLSEFAGKD
                     ALPIDYFMRSLNAKKMVAESMRSIDSHGLMMFTQFAKGVNAYIKGCKELPPEFILAGF
                     TPDEWQPEDGLYIFGMLNLDVSFNFIEELQFLMIAQKLGLEKACHLFPVYKDTELPFE
                     EAKKLRSIITTIPQIEEVSGTLSKLKQSLGLGIPASNNWAIAPTKTNGKSIVANDTHL
                     MASLPSPWIIMHLECPTYHCAGVCLPGIPIVVAGYNGHVAWGETMVMADTQDIFIEKI
                     KEEKGNLYYLYKGEWVPVEKHEEVFTVKGKKHTVTMYSTVHGPLLNMALLSMPFPPKL
                     PVQPPAVDMPYGLALSWVVAGGDKTLNGFYTLGKAKTLAQAREAMKHIQTIYLNIVYG
                     DSNSIGWQVTGLYPVRKKGTGLFPSPGWTGEYDWDGFVPFDRLPHVENPEEGFVVTAN
                     HRTVAKDYPVNLTQSWYNDERAMRITQVLSHNKTCTLEDVMKLQNDQYSLMAKHVQDI
                     LFSKDFNHLLMASLQKLSPKQKEKALKALQMLSPEKFDCIMRVDSAQAALMGALYHCF
                     VHNTFADELGPKDAPLWEAFLQASMTSYSAFDELLAYKHQSPFFDDVTTPEVETKWDV
                     LAKTLADAYVLCQDTMGRNEQKWQWGKLHAYWFKHEIAKEVGILKGFLSRGPYPAGGD
                     VHTVNVAMFTWGKDFDVWMIPAMRMIVDFNSNNPLHFIIVPGQSGNPVSRHYDDMVNM
                     YLHGTYQVIPMQGIKDPSGVEMLVP"
     gene            12556..13665
                     /locus_tag="PLV81_06775"
     CDS             12556..13665
                     /locus_tag="PLV81_06775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM09840.1"
                     /translation="MSREFFKNLERLAENAQDLIYRYELLPQPHFSYVSPAATTITGY
                     TPQEHYDNPNLGFEIVHPEDRHILQEMMEKRHFFEPVVLRWIKKDGTIQWTEQRNVPV
                     YDDNGQLIAIEGIARDITLRKQYEEEILRLNADLEQRVKERTAQLEEVNKELESFAYS
                     VSHDLRAPLTNIAGFARLLQEELAGKIEKNIESYITAINDSVKRMQKLIEDLLSFSRM
                     TRTELHHTQVDFTKMIQAIIHEVTMVQKSTAKFEVGNLPVMTGDEAMLRIAFTNLISN
                     AVKFSSKVQQPQVKIGSYENNGKTVIYVKDNGVGFDPKLSDKLFNVFQRLHAEKDFPG
                     TGIGLALVRRIVSRHGGRVWAQSEPGKGATFFVEL"
     gene            13941..>14259
                     /locus_tag="PLV81_06780"
     CDS             13941..>14259
                     /locus_tag="PLV81_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09841.1"
                     /translation="MPGDTRFIITPQIKKKIIEIIDERIGQVHVTKEDFSELKSIVRD
                     LAEAQKRTEEAQQRTEKRLEELAEAQKRTEEAQQRTEKRLEELAEAQQRTEKRLEELA
                     EAQQ"
     assembly_gap    14260..14359
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14360..15004
                     /locus_tag="PLV81_06785"
     CDS             <14360..15004
                     /locus_tag="PLV81_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09842.1"
                     /translation="EAQQRTEKRLEELAEAQQRTEKRVEELAEAQQRTEKRLGELTEA
                     QNRTEKRVEELSVSMKELAEAQKRTEEEIAKLSNGLRETRIQLGGLSRSFSYSFENEA
                     YRNLPQVLHQKYGYEVIEKMVRADIGGKEINFFGKALKDGKEFYIVGESKIRLDEGDW
                     RREVFADLESKIDAVRNVYGDVSILPILVTHFATNAFIDEAAQKGIIVVQSYEW"
     gene            complement(15321..>16793)
                     /locus_tag="PLV81_06790"
     CDS             complement(15321..>16793)
                     /locus_tag="PLV81_06790"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOM09843.1"
                     /translation="VDAGITIADALLEYGIVINTPCGGKGICKKCAVKATGALSQPDV
                     FEKSLGDSNYRLACRATLEGNVVVLADSTREYVHYPVLNPTHTYGVAVDIGTTTLQIA
                     FVDMTTDTVLPEITLLNPQRRFGHDVITRISHASDTTIAHKLTQLLLDAIISLITTAC
                     RLSRAETNIVKAIAFSGNTVMSYSLSGIDITPLGLYPYRTPVTTFDDYRNYRPLKEKF
                     PEATIIIMPVVSAFLGGDFLGGLGTLSQTGGNSFFFDIGTNGEMAIIKHDGSILATSC
                     AMGPALEGMNISSGMTATEGAITHFMLGNGKITFTAIGPPAGISGTALVDLIAILLEI
                     GVIDNSGKINSIPDAFANQIAIEHNDTIKTLNILNTITLSQLDIRNVQLAKGASLAAS
                     RILLKEAGLTEHDIDTVYIAGAFGKNVTIENFTRLQFLPHFTNAHFYIAGNTSLLAAI
                     EAIRNKDFIQKLAAIRSTITAIDLSLHQEFNDLYVQSLNF"
     assembly_gap    16794..16893
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16991..18124
                     /locus_tag="PLV81_06795"
     CDS             16991..18124
                     /locus_tag="PLV81_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09844.1"
                     /translation="MDATIISYPDTAVINLLSLTANRSKYMIPFAGKFRVVDFVIGNA
                     RELDAQTIIYSNVNDDLTHYVALHGIDESKLKVVVNEFNSIDFCYSLIRDSDSAYFIM
                     YNGDNPGIIDFAHLIERFAKMKKPAALFKMHFEGKARMAHRILVSTKKALLATIRQAL
                     KHEHTSPNIFEMIINMMVNEGLPKLEFSCMYWPLGTIVDYYNYNYEVINNPEFSKHVF
                     NGHIHSRIQLYRYSMLGPHAKVVQTFLSDSSYINGEVVNSIIFPGVEIAEGAVIRDSI
                     ILPYNKIGAKARIMRSIIDERTVQPFDQTPLTIGNGCKIGTTEEGMKNSDYPKVLYNS
                     ITLIGKNCIIPDAITIGGCCYVSPGKTQADFEKRKYIYNGTSV"
     gene            18126..18767
                     /locus_tag="PLV81_06800"
     CDS             18126..18767
                     /locus_tag="PLV81_06800"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOM09845.1"
                     /translation="MFLPYKDENPTQSFPLFTIVLIVLNCVVYYLEVYGQPGFENLIK
                     MYALIPCNVNRINVYNYNHYIPFISHMFLHVNIIHVASNMLFLWIFGNNIEDVLGVIR
                     FYGFYILCGLAAALTFYLFHHNECTPIIGASGAVSGLLGGYVVLFPAARIRVWFMFFI
                     LRLPAWFFILLWFVMQLSNLYGEKLGMTNVAWSAHVGGFIAGFILIIFFKKKY"
     gene            complement(19001..19786)
                     /locus_tag="PLV81_06805"
     CDS             complement(19001..19786)
                     /locus_tag="PLV81_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09846.1"
                     /translation="MISHVDPKVETPYNFWIENQQTNNFHPLEDHYGLSIGIGYAIND
                     WFSTGISFTAYSSYIRNQSDQGTIDLYNSMDGSFGLGFLFTLLNKRLYIDTSYTFNTE
                     QQYYLPPYTASTPPGDRVILEAHYPSIWEITITGVFLDRHLFLVLKQLTDFYTSAGAA
                     SSQYKREGWASRTIPSIEVWPFSWLSLRAGYIYQHTDLMEKTSSGQGGIGGLTLRFGT
                     WDFDVNYTVMERVSRLLPGYQTTDKRFLFQLTKHATFITTRQN"
     gene            complement(19791..>20315)
                     /locus_tag="PLV81_06810"
     CDS             complement(19791..>20315)
                     /locus_tag="PLV81_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09847.1"
                     /translation="WQDKITEKLSKYEYISAYFAGGILKVLNTTADTSTIAKLEKKEE
                     KNEKAVVAFSKAVDSLDKNDTEKARSQLEQAKKYDPENDVIKEYIAKLSVTSSKFKVE
                     LDFYVPSQNPAVLGTIQQDKMYWIFTISQKNLSGGKIEPVDVGDGYRLDETGNVINRL
                     GYEFPIGQRWGGKY"
     assembly_gap    20316..20415
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20416..21915
                     /locus_tag="PLV81_06815"
     CDS             <20416..21915
                     /locus_tag="PLV81_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOM09848.1"
                     /translation="ESADSSYDEALGVEYAKLVSLAMYIQKGIAIYSHDLLEKDLKEF
                     SIDPQGVVGSEDLNMLGKSRYIDRIQIGTLIKTKKGFAVKSVVYDVAAQKIVSRCSEY
                     ADTLFELANTEMRSLYLTIPDSTIAMGKNIYDVAFLIDNSYSAQREWKDIKRGIIALC
                     DSISDNWADMRVYIVPMLSQSKKIGTYAITSATTLDDHLQELSLNKGIVKSITPQLTY
                     IAKGIPWRKDAKKLCIILAASSCNYNEGRSLRFILKQNNVSVYAIGTGSLTHDDRVAL
                     SQLGDSYYDITYHQRMYDVNGNPVDVFCEAGRIFHGDAGVRWKNGVTTKTKAAKPFVA
                     EVFGTTAASPYELSSLYPRLSSIKILNSDELENNIIDICQTIAAASAVQGKEIARVLL
                     SDGGYSLWLPVSDAGVLTYLTQNQNVRMYVGISPKQDLGAPYGVGLQPFVVVGVPGSH
                     IPAMLKMTLKDIVQHKGFSRGLFNPPVWFVPVTVKQVMRYGSQDDIRNK"
     gene            22090..24702
                     /gene="clpB"
                     /locus_tag="PLV81_06820"
     CDS             22090..24702
                     /gene="clpB"
                     /locus_tag="PLV81_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOM09849.1"
                     /translation="MRMDKLTLKSQNALYEAQNIATQYNHPEIQPEHLLYALVNQEDG
                     IFRPIAQKLGAGVDDIKHEVTIALQSLPKQYGAGPGGGTMSGGLRDVLNNAWNEAVAL
                     KDEYLSTEHLILGLATTGKNKARDILNSHGFTKENILRVLADIRGNKRVTDENAEDKY
                     NALERYGRDLTKLARANKLDPVIGRDEEIRRVMQVLSRRTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVAGDVPESLKNKRVVALDMGALIAGAKYRGEFEERLKAVIQEVEESEGDVI
                     LFIDELHVLVGAGAAEGSVDASNMLKPALARGELRCIGATTLNEYQKYIEKDKALERR
                     FQPVFVGQPTVEDTIAILRGLKERYEVHHGVRISDSAVVAAAVLSNRYITDRFLPDKA
                     VDLIDEAASLIKMEIDSMPIELDEIERKIRQLTIEREAVKKEKDKASLERLAKIEEDL
                     ANLNEKKKELSVQWQNEKNIILEIRKIKEEIENLRAQEQKAEREGNLNLVAEIRYGKI
                     IEHEKKLKALNDKLTQIQKERKLLKEEVTEQEIAAVVSRWTGIPVSKMLEGEKEKLLK
                     MEEIIEKRVVGQEQAVQTIADAVRRSRSGLQDPNRPIGSFVFLGPTGVGKTETAKAIA
                     EFLFNDEKAIVRIDMSEYMEKHAVARLIGAPPGYVGYEEGGQLTEAIRRRPYSVVLFD
                     EIEKAHPDVFNIFLQILDEGRLTDSKGRTVDFKNTLIIMTSNIGTAYISDASIPYEQR
                     VKGVQQELKQHFKPEFLNRIDDIIIFNPLTREHINKIIMIQLNEVGKRLKERGISLEL
                     TPKALDYLVEVGFDPQFGARPLKRAMQRHLLNPLSVKILAGEVMDDDTVLVDFKNGQI
                     QFNVKRSKQEKKVV"
     gene            complement(24769..25362)
                     /locus_tag="PLV81_06825"
     CDS             complement(24769..25362)
                     /locus_tag="PLV81_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09850.1"
                     /translation="MKYTVKLIFAVLVCLTMVSFGMAAENLENRTRDIGLYGGVLLPG
                     TISIEDADVDTEMGFTLKAYFDQFVSPMFSVGVYGQYAATSTDSPEIVNPSVDVDVYE
                     FGLTFKPKFMISPTAAIKPGLEIGYRSTTVDLSQPGIDTSADGLALNFSAELQFALDG
                     GYLLGLTFGFLAQPTGGTDDADVTWAPIWYAMAGIVF"
     gene            complement(25447..26232)
                     /locus_tag="PLV81_06830"
     CDS             complement(25447..26232)
                     /locus_tag="PLV81_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09851.1"
                     /translation="MKRVLCFASLVLLIFISIPVVAQQNNFMISPPPIPWFEFKEGQK
                     DLRVTATGMYITGDSTPPLTGDVKVWGAGAGGIYRYAFKDILAFDVGLMLVGATGDVG
                     NEADMSMWLASLPLDLEILLVNNDQISLIGFIGFGFSRNYIGIDSNLPGYEGTVDITT
                     TMKGPQGGLQISFKLQDFTISPFVMITRQSGNVDMDYDIEGQGSGSISVTAPTITSRY
                     FGFDIVYVPLGLSLSSLMQQVSESGDNAGYKTYVINIGYCTQL"
     gene            26608..31005
                     /locus_tag="PLV81_06835"
     CDS             26608..31005
                     /locus_tag="PLV81_06835"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOM09852.1"
                     /translation="MDYEIYDVNNILLPVEHKIGALNRAKNLVAEMTHPNIDWKYMVT
                     ELRAYLYDYMYDIVPHSDKVLPVIFHYLKEATVRKRGSTLRAADTFLDRYLFLIKKEI
                     EGDSSLENVTAMFDNESIHFCQILIADTADGFYLEDVNLRILQLLELSLKRKKVDETL
                     FELCTEIIINQFKLYVERSIIVDDEEVYSLQNLWSIEHEHILKLEQLVKSVTKKAYQE
                     KLIKANALKNSKKDRATLLAEIKELIDFHHNTTSWEKICIAAKECIAQNVIEYDDVVL
                     ALLTFLVKKSQEGRDANLQLYISRSVASLCSVMVQQQRFVLLRQVVQMVVPVLVAEIE
                     RGGNYNAAFATILNIGKTVVQSDNRQIIDLFVDILVHAKFCFPQFTGIAQDWSVMVNA
                     SHLANIRTWLELIELNPVYMKRLAASLIANLTLGGVFLKDTDVFQRDISRLLNSNYKD
                     VFYLIISLAAVFPAFYHDIGATGNIRAFTERVDTNHQMNDLIHFVRKQVHVESSSRTV
                     VLLQRVMDFWLTGDKELLKGMVPQEVYNNLERTFRLINLDNESVARRIYTEIRHYFPE
                     LVYEKFWDFFYKVGKKRFMDVIAQHTFEGMDEDEKKDAIECIVEYFDKQFPAEMTKML
                     HHIAGMFDIDTSRRQIWRFLYEIPDDDFRKMFENVQKLDVSNVNIEKFITFLHVYRMI
                     FDKYNFSDIRAIEKLHQYAQENLFSPPENFFKRIEGNDDFDALEAILELQHTLKSDIL
                     LSQQVFEPVDTIEFKRHIAFGIPSMYGSYKEKKFDTLKVFFHCNIVRLLLFEKILENI
                     SIYPHQKVDYDAIKRVIKLFIQSFEIDGLANHEMRAVTSLLDAPNLTLTQFRDVIYSL
                     LVIHGEISDRFNDTFKSVSRIAIKNIGIDNIIHDFIPPDQPASIEVIVDRFLRNRVMQ
                     SPLLQLLDNLLLKLKDNLIHELSYLGNVVILNKVDTRIHKGRLVHIIGKYSVQHDETE
                     LFAPLWEVGAKAQGLIIAANIDGINVPEGLVISSELYKRIKDGNINNPRFKRKLIYML
                     KKYIDEFTGYRFGNPENPLLVSVRSGAVFSMPGVMDTITNVGMTEDIVPYFAQYDEWF
                     AWDCYRRVIHDFAISAFGMDRHIFENLMAQAKEEAGVDLKEKLNGKQMSLLTRKYRFA
                     INKAGYSVPKDPYEQLFYAIIAVFQSWDSAIAQNYRRFINLSDDWGTAVIVQRMVFGN
                     LSPTSITGVVHSQYIEYEDVQIAGEYKTRAQGHDIVSGVAKVFPISEQQKLKFARFAL
                     YPSMEKAYPGHYATLRDAVTRLRKRWDNDIEVEFTFENEKLYILQIRGMAKHMFDIEE
                     LVETPQELQEHLLGQGLAASGGAVSGRAVFDINRIEIMRMRYPKDKIILIRPETNPED
                     VIGLQKSDGILTSVGGMTSHAVLQMRRLEKSGVSDFSIMKIVEDENIAIINREQGGKI
                     IIREGDFITIDGNTGHVYLGFHPTRKAHR"
     gene            31183..32331
                     /locus_tag="PLV81_06840"
     CDS             31183..32331
                     /locus_tag="PLV81_06840"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HOM09853.1"
                     /translation="MAVSPDAPIGVMGIGRIGKLLVWLYAAKKQHKEIVIATGRKTGT
                     SLDDLATYFEYDSTYGTFDAFIGGFNRKNAVAVKDGHVYLNGVKLIWLNEPEFRVPGN
                     IPWNKYGVDVVFDTTGKMLDPTRNDDNSLRGHLNHAKKVILTAPFKVKDKGKQIPEDS
                     ITVVGGVNFDKYDGNKHAIISNASCTTNCCAPPIKALVDHFGDKFISYALTTVHAVTN
                     SQSTLDVLPKDGDKDTRKRRSILNNMIPTSTGAAKAVLEVIPEVQALGIGSQASSIRV
                     PVNTGSIVILDVSLLGDYDNKYIHNIFRQYSEKNPDIMYYSEKQLVSSDIIGSTYSTI
                     YDAEFTHHRTTKRGDNYYTMVDLNFWYDNEFGYVCSLSRLYDLVTGRR"
     gene            32469..32885
                     /locus_tag="PLV81_06845"
     CDS             32469..32885
                     /locus_tag="PLV81_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09854.1"
                     /translation="MKVCYPLIILLILIIFFAGSTYAKDVPYTLDDRDRIIRVEVKIN
                     EMDKRFEQIDKRFEQIDKRFDQIITFLWILTAIFITITSATLGFAFWDRKTVLKPIEE
                     KVISNEKKIDSALAAFRKLATVDKKVYAILKEFNLL"
BASE COUNT        10135 a         6021 c         6916 g         9698 t
ORIGIN      
        1 tgcagctatg ccttcacctg ttccggtaaa acccatccct tcagttgttt tacctttaat
       61 tgagatagat gatactggca aattaccgca ggcagctgaa agtatttctt tcatctgttc
      121 tgaatagggt gcaatcctgg gtttctggca tagtatgact gcgtcaatat ttataatttc
      181 tatattatgt ttggttaaaa gctgatatgt atctttaagc atatctatgc tgtatttgtc
      241 tttaaactgg ctgctggtgt cgggatatag cgtgccaata tcgcctaagt tgcttgcgcc
      301 aagcaatgcg tcaataattg catgtgttaa aacatcagca tctgagtgcc ccagcaggcc
      361 atattcatac ggtatatgca caccaccaat aataagcttt ctgccctgga caagtcggtg
      421 agcatcaaag ccaatgcccg ttctataagc tgtgagatta tgcatataaa ggttctggta
      481 taaggaacta tcgccaatta taaattaaaa acttcagcta gcgagcttaa tttcaataca
      541 ttaagcactt tggggctgac attgatgagt tccagttttt tgcctttttt cttctgtatt
      601 ttattcaggc tgataagaac gccaacacct gaagagtcaa tataatcaac ctcagaaaga
      661 tcaagtgcta tatcaacatc attgtcttca ccaactgcga ggagtttttc tttgaactct
      721 ttgattgaca tcatctcaat atcgccttta actttaacct caaccagatt tcctttattg
      781 ttaaggttga tttccatgtg gttacccctg tgtatattca agtcttacca gactaatata
      841 aacgcatacg tgctattgtg tcaataatta aatctttgtt acagtaaaaa tatatttgaa
      901 tgacatgtga ttaagaaata ataatcatta aaaaccgtgt ttactgccta aataattgac
      961 aaaattaaaa aataatgtag aatataaaca gtagtgttgg ctataggacc tgttaataca
     1021 atactacact aaggaacata caatgcaatt aaaaaaaaca tataccgatg tttatgaatc
     1081 aaaaaaaatt gatttccaga aactatgcga aattataaaa cctggttccc gtgttttttt
     1141 gagcagtggc ccggcaatgc ctgtgtatac tgttgaacag atgatgacat cagatgctcc
     1201 cacactgcag gacttggaga taatacatct tattacactg ggcaattacc ttaaaaccga
     1261 cagcaatctt tataaatttc gcctgaagac cttcaatacc ggtgaaagta tcagcaaaga
     1321 tattgagcag ggcaaggcag attttgtgcc tgcgcattta gtggaaattc catttatttt
     1381 ttccagtggt gctattggtg tggatgttgc tattatacag gtttcgccac ctgaccacag
     1441 ggggtttatg agtttgggca ttgcgataga tgttgccaat atagttattg aaaatgcaaa
     1501 aactgttgta gcagaagtca acccgtatgt tcctgttacg atgggtgaca cggctgtgca
     1561 tattgacgct atagattata ttattgaaag caaagtgccc cttattgaaa gggaatataa
     1621 gcgatacgat gaaataatgg ataagattgg atggcatgtt gccaatctta ttgatgatgg
     1681 ttcggtgatt gcattacatg ttggaagaca atttgatgcg atagcaaagc atctgcacaa
     1741 caaaagggaa ttaaaggttt acagccatgt tgtctctgac tggattattg accttattga
     1801 atcaggagct gtgaagatag accgaggaat acgcaattta gcacccgtga cgttgagcta
     1861 ttgttatggc acccggatgc tgtatgacta cgttgatcgt aatcccatgt ttgagttttt
     1921 accactcatg cgccttctat atcctaccgt tgtgcagcgc atacctaagc tggtgagtgt
     1981 catgaatgtc aagaagattg atctttctgg tgagtcagta gtattttact caggtgataa
     2041 tttactgtcc ggttatgaaa gcaagcttaa ttttgccgtt ggtgcagctt tttcccgtgg
     2101 gggcaaagct atagtggcat tacgttctat tgatcagcat ggcgatagta acattgtaat
     2161 acggcacaca gaaaccgaac aggtacgatc aaccttaggt gttacccgat atgtagtaac
     2221 cgaatatggt gtggcaaacc tttttggcaa atccattcgt gagcgggcac tggcgctgat
     2281 tgatattgct catcccaatc ataggctttc attattgaat caggctaagg aagcggggct
     2341 ggtgtatcct gatcagatat acgtaattga aaatattata aactatcctg taacccttga
     2401 aacaatgaaa acattcaagg atggtctgct ggtaaagttc aggcctatca agccatctga
     2461 tgaagatatg atgcgaaggc ttttttatca gttctcagat gaatcaaaat atctgcggta
     2521 ttttgcgcgt atacggacca tgccgcataa gcaaatgcag tcttacgtaa atattgacta
     2581 tgacagaata ttgtcgatag ttggcataat tcagcatgct ggcactgaac ggattattgc
     2641 agaagcgcgc tatggatatg atgacaatga aaaaacgtat gagatggctt ttattgttga
     2701 tgaggaattc cagggtaaag gtatagccac atttatggca cgatatttaa tgaagattgc
     2761 aaaggaacga aatattgaaa aaatgtcagc caatgtgttg ccgcaaaatg ataagatgat
     2821 gaaagttttt gaaaagtgtg gtgtgccgtt tacccagcgt tttgaagatg gcgtaatgca
     2881 ctttgatttt catttgcaga aagctgatat tgttatttaa tgagattgga tttagcagta
     2941 caggttgctt catcaatgat gaaatcacca aaaactgatg gtgcttcatc ttgcaaaatt
     3001 ttaagcatct gcatgcacac gcttcgtatt tcccactgtg ctgccttttc aaggcgcaaa
     3061 gaaaaaatat gtcgccactc cctgaaatta gctgaaatga ctatctggct ttccagtgcg
     3121 ttgggaagca caaaacgcgc atcttctttt tttatgctca gttcccgcaa ctttttatag
     3181 ataatgcgcg cctgttccat aaaatcagta aagatggttt ttgcttcatt atttgctgca
     3241 attgagggtg gaatgataaa attaaattct tcttcgttaa cgtagcgctg tgattgctgc
     3301 gaaaagcttg caagcctgtg tcgcaccatc tgatgggtga atgcgcggga agcccccagt
     3361 accctgaatg atgctgatgc atgttcaagt attgagtggt gaccgttcat gatgcaacgg
     3421 cgaatgaaat ttgtttcact tccttcggtt attctatcca gagaaaggta acaggtacgg
     3481 cctgcttcct caataagctt ttcagcattg ggtgttatag ctagtaaggt tacttttggg
     3541 gaataaattt ccatacacta tcgcttactt aaaaaatttt catgcccgaa aagcctaaaa
     3601 atgccattgc catgagacca gcggtaataa acgtgatggg cagaccttta aggttttccg
     3661 ggaccatggc aatttcaagg cgttcacgta tgccagccat aagaagcaga gcgagtgtga
     3721 aaccaacgcc agcaccaaaa ccctgcacca cagaattaag caggccaaac tcctgtgtaa
     3781 taaagcctga ttctatatta agaacagcta ctcccaacac agcacaattg gttgtaataa
     3841 gtggtaaaaa tattccaagc gcctgataca gggcagggga tattttctca ataaccattt
     3901 caacaagctg tacaagagaa gctataacaa taataaacgc aatggttcgc aaatattcaa
     3961 tatgaaaagg tataagcaca aatttgtaaa taataaaggt aaagaaagaa gccattgtca
     4021 taacaaagat aacagccagc cccatgccaa tggccgaatc caatttttta gaaacaccta
     4081 aatagggaca aaggcccagt attcttgaaa gaacataatt atttataaag atagtactta
     4141 ttagtattgt aaaaagtatc ttaatttcca taacgatagt acctcacctg tacttttatt
     4201 cagtacgttt aaaaagagct ttccgtgatt ttataacatt catagcccac aggagcagtc
     4261 ctaaggtaaa aaatgcacct ggtgcaagta tcattagaat tgctggcttc atgccaggaa
     4321 ttaaaacata tccaaaaagc ttatttgcac ctaacacctc gcgaattgtt gcaaggataa
     4381 agagggttaa accaaacccc aaccccatac ctaatgcatc aagaattgat gatagaactg
     4441 aattctttga agcaaactct tcggctcttc ccaatatgat gcagtttact acaatgagtg
     4501 gaataaatat accaagtgct ttgtatacat caggctgaaa tgccttaagt gttaattcaa
     4561 caatgataac aaagctggct attatggtga taaaaattgg tattcgaaca tgtgaaggga
     4621 caatggtacg tatggccgat acaaggatat tggaccccag taatacaaac gttgttgcta
     4681 ttgtcatcca gatagcattg gatgctgaag tagtaactgc aagtgttgga cacatcccta
     4741 tccctaatac caggattgga tttttttgcc atactccttt aaaaaattcc ttaaagtaat
     4801 ccattattgt acctcttgca gtgttaaagc tttatgtaat ttttgtatgc cttcttcaat
     4861 actctgtgta actgctttag tggtgatagt ggcccctgtt attgcagtaa cggcattatg
     4921 ctgaattaac tcatctttca tttctttgtg ccagtcgcct tttttgtgaa tagcaatggg
     4981 tttgctggca ttcaagccaa taaattgttc ctgaaaccag gacacctctt ctttactggt
     5041 aagatttttt gttataaaat ggtcaacaaa tgtttcattg gatgcaacct caacacagcg
     5101 tgcaccaagt cccggagttt cattctgtga tagtatggta aaacctaaaa ttgtatttga
     5161 ttcatctacc ccaacaattg attgaactgg tccgccgtat cctttttttt cagctaaaaa
     5221 agcccatgct ttttttacta tcctgttttc ctcttttgtt gctatccagt aagtaaatgg
     5281 agaatcatca atggtaactg ttttttcctg ctgtactgta taaccgggta atacaagagc
     5341 aagagccgcc agcttctttt cctgttcctg ttttgctatt ggttcctgag taatcttttt
     5401 cataaatgat aatgaaaaag cagatataaa tccaataaca cttataataa tcgtagcctt
     5461 tacgatatca gcaggtttca tgtaataatt gttcctctca tcttaaaaat atgatgtata
     5521 gcaattatca ttttaaatcc cgatttcatc ggggtgagaa atctttttaa cagcgaaggt
     5581 catcctgagg aatcccaact ttatcgggac gacgaaggac cttgtcctta ctactcagag
     5641 attcttcgct gcgctcagaa tgacaaagaa attaaatacg atttctcctc acttggttcg
     5701 tcgaaatgac agtatgaaaa attacttcgt tcgtcttagt aacagcgtaa aaataatcat
     5761 agtgacagag caaaaatgtt cgtcgaaata agtaattcca aaaatattat aatcagttac
     5821 tatcgggtac caaatacttt tggtcgtgtt aacctgtcaa ttaacggtgt taccgcattc
     5881 atcaaaagaa ttgaatatga tacaccttca gggtatccac cccacaggcg tatgaccgaa
     5941 gccagtatgc cacatcctat gccaaatata atcatccctg tttttgtaac aggtgaggtt
     6001 accatgtcgg ttgccataaa aaaggcaccc aaaattagtc cgcccgacag tacatggaaa
     6061 agtattgcat actgaagatt ggggatgccc tgaaaataat agtatattcc cataattagt
     6121 gcaaccgttg ctatatagga tacgggtata tgccaggtaa tgatgcgttt ccataaaaga
     6181 agtagtgcac caattaataa cagcaatgct gatgtttcgc caatgcagcc gccaatattt
     6241 ccaataaaca atgacttgat cattgctttt gaaaaaagga agtcatacaa cggctgcgtt
     6301 tggagcgata gttcatgtaa tacctttggc ccttctttca aagcgcccag tggcgttgct
     6361 ccactgatgc tatctacgag ctgttgtggc atagatggat caaatattgg attctgagta
     6421 ataacatgac cgccagggaa tttatgccat agagtggtca tttctaccgg ccaggatgcc
     6481 aagaggaacg ctctgcccgc aagagcagga ttgaatatat tgtatcccaa tccgccaaac
     6541 aattgctttg ctatgatgat agcaaatgca gaacctattg ctggaatcca tagtggaacg
     6601 tgtggcggaa gattcatggc taaaagaagc ccggttatca cagctgaacc atcatttatg
     6661 gtaatgggtt tttttagcaa gtactgcatg atgctttcag aaatcagtgc cgatataatt
     6721 gacaccgcaa taacaagtgc aacatataat ccgtatatat agcatgaaaa tactgttgct
     6781 ggcaacaatg caagcactac cagccacata acaagcggaa ctgattgcgt atcgcgaata
     6841 tgtggcgcat gtgaaagaac taaatcctct aacaggcgtt ttttgtcagc atcttttatt
     6901 tccatgaaca acccttctac attcttatat tatcgttttg cactgagtac tgtctggact
     6961 acctttataa aatgcgacaa tgggcgtttt gacgggcata cgtatgaaca tgaaccacac
     7021 ataatacaat caaatgggtg tagcttttct gcaatatcat agcgctgctt ttcaacagca
     7081 ttgcctaaat cacacggcat aaggttcatg ggacataccg ctacgcacct gccacagcgg
     7141 atacatggtc tgaacgaatc cacttccact tcgtcttttg ctaaaaataa aatgcccgat
     7201 gtacccttaa ctattggtat atccatgtgg ttaagtgcca gtccacacat tgggccaccc
     7261 atgatgatct ttgcagggtt ttcagtaaga ccaccacact cttcaagtat gtcaataacc
     7321 cgggtgccaa accgcacctt atagtttcca ggtttctgta caattttgcc tgttactgtg
     7381 atgtagcgtt caattaatgg tttgtcaaac aatactgctt cacggattgc atatatggtg
     7441 cctacattct gtacaaccac attgacatcc atgggcaatc cgccggaggg aacctcgcgt
     7501 cctgttatgg tatagataag ctgcttttca gcaccttgag gatagcgtgt acgcaaaggc
     7561 actacactga tatcttcaga ttttatactg gttacagctg tctgtaatgc ttttatagcc
     7621 agagctttgt ttttctcaac accaataaat gcttttgata cacccagtat cttcatggat
     7681 atctggattc cttcaatgat ctcttctgga tgtgtttgca taagcatatc atcaacggta
     7741 aggtaaggtt cgcattctga accgttgatt atcagtgtgt cgatagttct gttggctggt
     7801 gggttcaatt taacagcagt tgggaaagca gcacctccaa gaccaacgat tccagcagcc
     7861 tttacttttt caagtattgc atccctatcc agggctgtca tatcgttttt ttcacggact
     7921 ttgccactcg ttgaaaatga accttcagct tcaatgacaa cgcaaaggcc gtgttcactg
     7981 tatggtgtat ggtattcggc tatctcaaca acttttccgg gtaccgttgc atgcacattg
     8041 gcactaataa gaccgtttgc ctggccaata agctggcctt cctctacaaa ttgtccaacc
     8101 tcaactacgg gtgtggcagg tttaccaata tgttgctgca aaggtatgta acatttttga
     8161 gggacagata aatacttaaa tggtatacgg ttggtaaaat gtttattctc aggtggatga
     8221 attcctccgt gaaagctatg aatcatcata ggttattccc tggttgagta gatttcatct
     8281 ttaataggaa gatctttcct taaattctta atatatggat aaaggtcacc tgcttcagta
     8341 taatactgac aattatccac cgtacggcgt gacactgtaa aatacttgta taacttttct
     8401 tcaccagagc gcttggtcca ttttttcttg gtacagcgaa ctcttaaaac aaaacgctca
     8461 gggtcagatt catattgacg aatcacaata caattcatgc aattagcgca aaaacatttt
     8521 tctttaggca ttgcgctctc ctatacttta atatttaaga aaacaatgta aaaaagacta
     8581 tcactgttat gtttatataa aatcaataat tttttttaag taaggttaaa aaaagtttgc
     8641 aaattcagac ctataatata atacatattg ttattcttac tgctccaatg aagtcaatga
     8701 ttaaatccca atgctggaat atggccactt ctttttattc cagctgtatc tcattactca
     8761 atttattacc tattgtgctg ttatcaatat tatttttatt gactaatgtg ttatctgtgt
     8821 agtatattga aatagtgaag tagtattata tggaattaat gcaaccgggg tggattcatg
     8881 ggatatgcag taagaatata tgaagccttt aaggaagatg aggtaaaggc aaaagtactg
     8941 gcggaattta tagaaaaagt ggaagaagct ataaataata atcaaatagc aacacgacag
     9001 gatttaaatg tccaggagtt aaaactcacc aaagaaatta aagaagttga actacggctt
     9061 acaaaagaga tagaacgggt tagagctgac ttgacaaaag agatagagca ggttagagct
     9121 gacttgacaa aagagataga gcaggttaga gctgacttga caaaagagat agagcaggtt
     9181 agaggtgaat tgcgactgga aatagaaaaa gtgcgtgtag aattgcacca ggtaaaagca
     9241 agcatcttaa aatggctcat tggcttactc cttgcccaga ctgttacaat aattggagtt
     9301 attgtgggat tatttcaagc tatgaaatga tctcattgtt tgtacggtaa cacttttatc
     9361 ctctattttt ttaaaatgta tataaactat ttgttagtaa agaagggtcg tatttttaca
     9421 tttataaata ctatagagct tctggactcc tgttggcaaa attccctctt tgagatgaat
     9481 atcctggctt gaccggagtg gttaacctct tgttggccag aggtaatagc atttagtcag
     9541 aaaaatttta tcctacaggt attatttaat tgacatagta gcaaaataat atgaacatcc
     9601 gttctatatt tgaaccattg taaaaatacc aattatttaa gtgtatgccg attatgcagg
     9661 gaagtatgaa tcagaatacc agcgtttagt tcttgaaata tgaaatggtg acaatctata
     9721 taacggatac tagtattgat attctatttc tcaatcagat gggcatgctg gcatagaatg
     9781 tagtaagaat tatgaaaatc atgatttttt cccatttata ctgcaggttg ttttgtgaat
     9841 attgcaatca tgtacatggc gcgatgtttg tagtagatcc atgataaaag cttataaatg
     9901 ttggagaaaa gtattgcaga atatgataag caaaatgggt taagaaatat acatttgctt
     9961 aagcctgaaa acttttttta cgggaggaaa ttaaggtatg gtaaaaaatc ttagccgtat
    10021 catcatactg atcatgatgc cagcattgat ttcacaatgt tccataatgg gacattattt
    10081 caaatcatca gaaaaacagt atagcggtac catacacatt gaagggttga aacaaccggt
    10141 agaggtgcgc tacgataatc taggcattcc tcacataaag gcgcacaccg aagaagattt
    10201 gtttttggca accggatatg tcatggcatc ataccggtta ttccagatgg ttatgataaa
    10261 gatggctatt cagggaaggt tatcagagtt tgcaggcaag gatgcgctgc ctatcgacta
    10321 ctttatgcga agcctcaatg caaaaaaaat ggtagccgaa agcatgcggt caattgacag
    10381 ccatggcctt atgatgttta cacaatttgc aaaaggggtc aatgcttata tcaagggatg
    10441 taaagagtta ccaccggaat ttatccttgc aggatttaca cctgatgagt ggcagcctga
    10501 agatggtttg tatatttttg gtatgctgaa tctggatgtt tcgtttaatt ttattgagga
    10561 gctacagttt ttgatgatag cacaaaagtt aggtctggaa aaagcatgcc accttttccc
    10621 cgtttacaaa gatactgaac tcccttttga agaagcaaaa aaattgcgta gcattataac
    10681 cactatccca caaatagaag aagtttcagg taccctaagc aagctaaagc agtccttagg
    10741 attgggtata cctgcgtcaa ataactgggc tattgcgcca acaaaaacca acggcaaaag
    10801 tattgttgca aacgatactc atttaatggc atcgcttcct tcaccgtgga ttattatgca
    10861 ccttgagtgt cctacctatc actgcgctgg agtatgtttg ccgggtattc ccattgttgt
    10921 tgcaggctac aacggtcatg tggcctgggg ggagaccatg gttatggctg atacgcagga
    10981 catattcatt gaaaagataa aagaagaaaa gggtaacctg tattacctgt ataaaggtga
    11041 gtgggtgcct gttgaaaagc atgaggaagt atttacggta aaggggaaaa agcatactgt
    11101 taccatgtac agcacggttc acggcccgct acttaatatg gctcttttat caatgccatt
    11161 tccaccaaag cttccagtgc agccgcctgc agttgatatg ccctatgggc ttgcactatc
    11221 ctgggttgtg gcaggtggtg ataaaacact caatggattt tatactttag ggaaagctaa
    11281 gacacttgct caggcacggg aagcaatgaa acatattcaa acaatctatc ttaatattgt
    11341 atatggcgat agtaactcta ttggctggca ggtaacgggg ctttatcctg tgcgaaaaaa
    11401 aggcaccggc cttttcccat caccaggatg gacgggtgaa tatgattggg atggctttgt
    11461 tccgtttgac cgattacctc atgtggaaaa tccagaggaa ggatttgtgg taacggcaaa
    11521 tcatcgtact gttgcaaagg attatccggt gaaccttact cagtcatggt ataacgatga
    11581 gcgtgccatg cgcatcactc aggtactatc gcacaataaa acatgtacgc ttgaagatgt
    11641 tatgaaattg cagaatgacc agtattcact gatggcaaaa catgtgcagg atattttatt
    11701 tagcaaagac tttaatcatt tgctgatggc ttcattgcag aaactatcgc ccaaacaaaa
    11761 agaaaaagct ttgaaggcgc tgcagatgct ttccccggag aagtttgatt gcatcatgcg
    11821 tgtggatagt gctcaggctg cactgatggg tgcgctgtac cattgttttg tacacaatac
    11881 gtttgctgat gagcttggac caaaagacgc gccgttgtgg gaagcatttt tgcaggcaag
    11941 catgacatca tattcggcat ttgatgagct tcttgcgtat aaacaccagt caccattttt
    12001 tgatgatgtg acaacacctg aagtggaaac caaatgggat gtgcttgcaa aaacgttagc
    12061 tgatgcgtat gtgttgtgcc aggataccat gggaagaaat gaacagaagt ggcagtgggg
    12121 gaaactgcat gcatactggt ttaaacatga aatagcaaaa gaggttggca ttttaaaagg
    12181 tttcttaagc cgtggtccct atccagctgg aggtgatgtg catacggtta atgtggccat
    12241 gtttacctgg ggcaaagact ttgatgtgtg gatgattcct gccatgcgta tgatagttga
    12301 ctttaacagc aacaatccct tgcatttcat tattgtgcca ggccagtcgg gaaatcctgt
    12361 tagcaggcat tatgatgaca tggttaatat gtatttacat ggcacctatc aggttattcc
    12421 catgcagggc attaaagatc cttcaggagt tgaaatgctg gtgccgtaaa ttaagagatt
    12481 tgaattcatg aaattttttt gagagatagt aagggtaagt acctgtatca agcattgtga
    12541 ataggaatgt aagaaatgag tagagaattc tttaaaaatt tagaaaggct tgccgaaaat
    12601 gcacaggatt taatctatcg ttatgagcta ttgccacagc cacattttag ttacgtaagc
    12661 cctgcggcaa caaccattac tggctatacg ccacaggaac attatgataa tcccaatttg
    12721 ggctttgaga tagtacatcc tgaggacagg catattttac aggaaatgat ggaaaagcgg
    12781 catttctttg agccagtggt gttaagatgg ataaagaaag atggcactat acagtggacc
    12841 gaacagcgca atgtaccggt atatgatgac aatgggcagc ttattgccat agaaggtatt
    12901 gcgcgtgata ttacactgcg taagcaatat gaggaagaaa ttttaaggct gaatgctgac
    12961 ttggagcaac gagtaaagga gcgcacagca cagttagaag aggtgaacaa agaacttgaa
    13021 agctttgcgt acagtgtatc acatgatttg cgtgcaccgc ttaccaacat tgctggattt
    13081 gccagattac tacaggaaga gcttgctggt aagattgaaa aaaatataga aagttatata
    13141 acagccatta atgattcagt aaagcgtatg cagaagctga ttgaggattt gctttcattt
    13201 tcacgtatga cacggactga acttcaccac acgcaggttg attttacaaa gatgattcag
    13261 gcaattatcc atgaggttac tatggtgcaa aaatctacag ccaagtttga agttgggaat
    13321 cttcctgtta tgacaggtga tgaggcaatg cttcgtattg cttttaccaa tcttatttcc
    13381 aatgcagtca aattttcttc aaaggtacag caaccccagg ttaaaattgg tagctacgaa
    13441 aacaatggta aaacggtgat ttatgtgaaa gataatggtg ttgggtttga tccaaaacta
    13501 tcggacaaac tttttaatgt atttcagcgc ctgcatgcag aaaaggattt tccaggtacc
    13561 ggtattggcc ttgcgctggt gcgaaggata gtatcacggc atgggggcag ggtatgggca
    13621 caatcggagc caggcaaagg tgctacgttt tttgttgagc tgtaagatgc acaaaaaaca
    13681 tcttgatttt ttaaacacgc gtactatttt agaattagaa atttggatat agtgatatta
    13741 ataattgtgt tattagtatt ttcgtgtaaa tattcataat attaacacta tatgtaaata
    13801 ttgtgaaaaa acaattgtta aggaagtata ctttttttat attttattga tttttggctt
    13861 gaaaaatttt tgaagattac ataactgcaa tggcaaccaa aaaaatttta acagaaaaaa
    13921 taaaaaggaa gggaaaaagt atgccaggag atactcgttt cattatcaca ccgcagatta
    13981 aaaaaaagat tattgagata attgatgaac gaattggaca ggtacatgtt acaaaagagg
    14041 atttttctga attaaaatcc atagttcgtg atctagctga agcgcaaaag cgtacagaag
    14101 aggcacaaca gcgtactgaa aagcgattgg aagaattagc tgaagcgcaa aagcgtaccg
    14161 aagaagcaca acagcgtact gaaaagcgat tagaagaact agctgaagca caacagcgta
    14221 ctgaaaagcg attagaagaa ctagctgaag cacaacagcn nnnnnnnnnn nnnnnnnnnn
    14281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14341 nnnnnnnnnn nnnnnnnnng aagcacaaca gcgtactgaa aagcgattag aagaactagc
    14401 tgaagcacaa cagcgtaccg aaaagcgagt agaagaacta gctgaagcac aacagcgtac
    14461 cgaaaagcga ttaggagaat taactgaagc acaaaatcgt accgaaaaac gtgtagaaga
    14521 attatctgta tcaatgaaag aattggctga agcacaaaaa cgcacagaag aggaaatagc
    14581 taagttatcg aatgggttgc gagaaacacg tattcaactg ggtggtttat cacgcagttt
    14641 tagctattca tttgaaaatg aagcgtatag aaacctgcca caggttttgc atcagaaata
    14701 tggatacgag gttattgaaa aaatggtgcg tgctgacata ggtggcaaag aaataaattt
    14761 ttttgggaag gcacttaaag atggcaaaga attttatatt gtgggtgaat caaaaataag
    14821 attggatgaa ggtgattggc gtcgtgaggt atttgcagat ttagaaagca agatagatgc
    14881 tgttcgtaat gtatatggtg atgtatccat acttcctata ctggtaacac attttgcaac
    14941 aaacgccttt atagatgagg ctgcacaaaa aggaattatt gtggttcaaa gttatgagtg
    15001 gtaattaaaa tagcagcggg atatggcgtg ccatatattc atagaaatac gcggtgttct
    15061 gtggcgatag tacctgttag cggtagcttt ggaattgttc ttatgaaaga atttgattaa
    15121 attatgtatt ataaaaagat aatctaaatt gataaaccaa tttatcgtgt atggttaaaa
    15181 atttgttcta atttgataaa ttatctcaag acaaaaaagc caaagaaaaa tgaaaaacta
    15241 acatttgaga ttgtaaaaat cagggatgat ggcggacagg taagcaaaac tactgtgata
    15301 aaggatctga ttgtaaaaaa ttaaaaattc agtgattgta catacaggtc gttgaattct
    15361 tgatgcagtg ataaatctat tgcagttatt gtacttctga ttgcagcaag cttctgtata
    15421 aaatctttgt tgcgtattgc ttcaatagct gccagaagcg atgtgttacc agcaatataa
    15481 aagtgtgcat ttgtaaagtg tggcaaaaat tgtaaccggg taaaattttc aatggttacg
    15541 tttttaccaa atgcaccagc tatatacaca gtatcgatat catgttcggt taagcctgct
    15601 tcttttaaga ggatacgtga agcagccagg cttgctcctt ttgccagctg aacgttgcgt
    15661 atgtcaagct gtgatagtgt aattgtattc aggatattga gtgtctttat ggtatcattg
    15721 tgttctattg caatctgatt ggcaaatgca tcaggaattg aattaatttt accgctattg
    15781 tctataacgc caatctccag taagatggca attaaatcca ccagggctgt tccactaatg
    15841 cctgcaggtg gccctattgc ggtaaaggta atctttccat ttcccagcat gaaatgagtt
    15901 attgcacctt ccgttgcggt cataccgctt gatatgttca taccttcaag tgctggtccc
    15961 atggcacatg atgttgccag tatgctaccg tcgtgtttta tgattgccat ttcaccattg
    16021 gtgccaatat caaaaaagaa actgtttcca ccggtttgcg acagtgtacc aagcccacct
    16081 aaaaaatccc cacctaaaaa tgcgctcaca acgggcataa taattattgt agcttctgga
    16141 aatttttctt taagggggcg atagttccgg taatcatcaa atgtagttac aggtgttctg
    16201 taaggatata gtccaagcgg tgtgatgtca atgcctgata atgagtaact cataacggta
    16261 ttgccggaaa aagcaattgc ttttacgatg tttgtttctg ctctggatag acggcaggct
    16321 gttgtgataa gtgatataat tgcatctaac agaagttgtg taagtttgtg tgcaatggtt
    16381 gtatcgcttg catggcttat gcgggtaatg acatcatgac caaaccttcg ctgtggatta
    16441 agcagtgtga tttcaggaag cacagtatcg gttgtcatgt caacaaatgc aatctgtaag
    16501 gtagtggtgc caatatcaac agcaacaccg tacgtatgtg tgggatttaa tacaggatag
    16561 tgtacatatt cacgggtgct atctgccagt actactacat tgccttctaa tgttgcgcgg
    16621 caggctaatc tatagttact atcgcccaaa cttttttcaa acacatcagg ctgtgaaagc
    16681 gcacctgtgg cttttactgc acatttttta catattcctt taccaccgca cggtgtattg
    16741 ataacgatac cgtattcaag caacgcgtca gcaatggtaa tgcccgcatc aacnnnnnnn
    16801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttacttt aaattatttt tttatttaca
    16921 gatgaaaagt ccttgctatg aaacaacagt atcacataat gttgctttgt aaggagtaat
    16981 cgaagtgtca atggatgcaa ctatcatatc atatccggat acagctgtta ttaatttact
    17041 atcgcttacc gcaaacaggt caaaatatat gattccgttt gccggtaagt tccgcgttgt
    17101 tgattttgtt ataggaaatg caagggaact ggatgcacaa accataatct atagcaatgt
    17161 aaacgatgat ttaacccact acgtagcgct gcatggcatt gatgaaagca agcttaaagt
    17221 tgtggttaat gaattcaaca gtattgattt ttgctattcg cttattcgtg atagtgattc
    17281 agcatatttt ataatgtaca atggcgataa tcccggcatt atagattttg ctcacctgat
    17341 tgaacggttt gcaaaaatga aaaaacctgc tgcactcttc aagatgcatt ttgaagggaa
    17401 ggcacgtatg gcgcaccgta tactggtaag cacaaaaaaa gcgttgcttg caacaatcag
    17461 gcaggcattg aagcatgaac atacttcacc taacatcttt gaaatgatta tcaatatgat
    17521 ggtaaatgag gggttgccaa aacttgaatt ttcatgtatg tactggccac tgggcacgat
    17581 agttgattat tataattaca attatgaggt tatcaataat cctgaatttt ctaaacatgt
    17641 tttcaatggc catatacatt ccaggataca gttataccgc tattcaatgt tggggcctca
    17701 tgcaaaagtg gtgcaaacat ttttgtccga tagttcctat attaacggcg aggtggttaa
    17761 ttcaataatt ttccctggtg ttgaaattgc agaaggtgca gtcattcgtg attcaattat
    17821 cctgccatat aataagatcg gtgcaaaagc gcgcatcatg cgaagcatta tagatgagcg
    17881 cactgtgcag ccatttgacc agacaccgct taccatcggg aatggttgta aaattggcac
    17941 aaccgaagaa ggtatgaaaa acagcgatta ccctaaagtg ctgtacaaca gtataacatt
    18001 aattgggaaa aattgtatta ttcctgatgc tattaccata ggtggatgct gctacgtatc
    18061 gccgggtaaa actcaggctg attttgaaaa acgaaaatat atctacaacg gtacttcggt
    18121 gtgacatgtt cttaccgtac aaagatgaaa atcctacaca gtcatttccc ctttttacga
    18181 ttgtacttat tgttctgaat tgtgtggttt actatcttga ggtttacggg cagccaggtt
    18241 ttgaaaactt gataaagatg tatgcactaa ttccctgcaa tgtaaacagg attaatgtat
    18301 ataattacaa tcattatatt ccttttataa gccacatgtt tttacatgta aacataatcc
    18361 atgtggcatc taatatgctc tttttatgga tatttggcaa taatattgaa gatgttctgg
    18421 gggttatccg gttttatggg ttttatattt tgtgtgggct tgctgctgct ttaacgtttt
    18481 atctttttca ccataacgaa tgtacaccta tcattggtgc ttcaggtgcg gtatcggggc
    18541 ttttaggtgg gtatgtggtg ttgtttcctg cagctcgcat ccgggtgtgg tttatgtttt
    18601 ttattttacg tctgccggcc tggtttttta tattactgtg gtttgtcatg cagctttcca
    18661 acctgtatgg cgaaaaatta ggtatgacca atgtagcctg gtcggcacat gttggcggtt
    18721 ttattgcggg atttatttta attatttttt ttaaaaagaa atattaatgt atgcatcaga
    18781 ctggccgttt ggatataggc caccagcaat taaaactgtg aaattggcat gcaaaggcga
    18841 tacgacactt gaagatatgt tgttttataa aaatgccgct atgctcatgg cgattacaat
    18901 ttaatataat tgctatatat tttctgaaca atagcttatg aatacaatat aacatattat
    18961 acacaccagt tgccagagac aactggtgtg tatattactt ctaattttgt cgagttgtta
    19021 taaacgtggc atgtttagtt aactgaaata aaaatctttt gtcggtggtt tggtatcctg
    19081 gtaataatcg tgaaacacgt tccatgactg tgtaattgac atcaaaatcc catgtgccaa
    19141 agcgcaacgt taatccacca ataccaccct ggccgctgct ggttttttcc atcaagtcag
    19201 tgtgctgata aatatatcca gctctcagtg atagccagct aaaaggccac acttcaatag
    19261 atggaatggt acggcttgcc cacccttcac gcttatactg gctgcttgct gctcctgcac
    19321 tggtataaaa atcggtaagc tgttttaata ccaaaaacag atgcctgtca agaaatactc
    19381 ccgttatggt aatttcccat attgatggat aatgtgcttc taaaattacc ctgtcacctg
    19441 gaggtgttga agctgtatat ggtggtaaat agtattgttg ttcggtgtta aaggtataag
    19501 atgtatctat gtataatcgt ttgtttaata aagtgaataa aaatcctaaa ccaaatgaac
    19561 catccatgct attgtataaa tctattgtac cttgatcact ttgattacgg atataacttg
    19621 aataggcagt aaatgatatt cctgtactaa accagtcatt aattgcatat cctattccaa
    19681 tggataaacc ataatgatct tccagaggat gaaaattgtt tgtttgctgg ttttctatcc
    19741 agaaattata cggtgtttca acttttggat caacatgtga aatcattgcc tcaatattta
    19801 cccccccagc gctgtccaat ggggaattca tatcctaagc ggtttatcac attacccgtt
    19861 tcatctaacc tatatccatc gccaacatct actggttcaa ttttaccacc acttaagttt
    19921 ttctgactta ttgtaaaaat ccagtacatt ttgtcctgtt gaattgtacc aagcacagcg
    19981 ggattctgtg agggtacata aaaatccagt tctactttaa attttgatga tgtgacagaa
    20041 agttttgcaa tatattcttt tataacatca ttttcagggt catacttctt tgcctgttcc
    20101 aactggctac gagctttttc tgtatcattt ttgtccaggg aatcaacagc ttttgaaaaa
    20161 gcaacaacag ctttttcgtt cttttcttct ttcttttcca gttttgcaat tgttgatgtg
    20221 tctgcagttg tattgagtac ttttaatatt ccgccggcga aataagcact gatatattcg
    20281 tattttgata atttttcggt aatcttatcc tgccannnnn nnnnnnnnnn nnnnnnnnnn
    20341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20401 nnnnnnnnnn nnnnngaaag tgctgatagc tcttacgatg aagcgttagg tgttgaatac
    20461 gccaaactgg tttcactggc aatgtatata cagaaaggca ttgcaatata ttcgcatgac
    20521 cttcttgaaa aagatttaaa agagttttcc attgacccgc agggtgttgt gggaagtgaa
    20581 gatcttaata tgttgggcaa atcccgctat attgaccgca tacagattgg cacactgatc
    20641 aaaacaaaaa aaggatttgc cgttaaaagc gttgtatatg acgttgccgc acagaaaata
    20701 gtgtcccgat gcagcgaata tgctgacaca ttgtttgaac ttgcaaacac tgaaatgcgt
    20761 tcgctttacc ttactattcc tgacagtaca attgccatgg gcaaaaatat atatgatgtt
    20821 gcattcctca tagataattc atacagtgca cagcgtgagt ggaaggacat aaagcgcggc
    20881 attattgcgc tatgcgatag catcagcgat aactgggctg atatgcgcgt ctacattgtg
    20941 ccaatgcttt cacagtcaaa aaagattgga acgtatgcaa ttaccagtgc taccacactg
    21001 gatgatcact tacaggaact atcgctcaac aaaggaattg taaaatccat tacaccgcag
    21061 ttgacctata ttgcaaaggg aataccctgg cgcaaggacg caaaaaagct ttgtattata
    21121 ctggcagctt catcatgcaa ttacaatgaa gggcgttcac tgcgctttat acttaaacaa
    21181 aataatgtca gcgtgtatgc tattggtaca ggatcgttaa cgcatgatga ccgtgttgcg
    21241 ctttcacaat tgggcgatag ttactatgat ataacctacc atcagcgcat gtatgatgta
    21301 aatggcaatc cggttgatgt cttctgtgag gcaggaagga tcttccatgg tgatgccggt
    21361 gtgcgctgga aaaacggtgt gaccacaaag actaaagcag caaagccatt tgttgcagag
    21421 gtatttggca caactgctgc ttctccgtat gaactatcgt cactgtatcc ccgactatca
    21481 tccattaaga ttcttaacag cgatgaactg gaaaacaata tcattgatat atgccagact
    21541 attgcagcag caagcgcagt acagggcaag gaaatagcac gggtgctgct atctgatggt
    21601 gggtactcgc tgtggcttcc ggtttctgat gctggggtac tgacatatct tacgcagaat
    21661 caaaatgtgc gaatgtatgt aggcatttct ccaaaacagg atttgggtgc tccgtatggt
    21721 gtggggttgc agccgtttgt tgtggtgggt gtgccgggaa gccatatacc tgccatgctc
    21781 aaaatgacac ttaaagatat tgttcagcat aaaggttttt cacgggggct ttttaatccg
    21841 ccggtatggt ttgtacctgt tacagttaaa caggttatga ggtacggcag ccaggatgat
    21901 atacggaata aataatactt gacgaatgga aataaattga gtatattttt aatacataca
    21961 cgttttataa ctccttgctt cctgtggcgg caggggaata catatacata taattacctt
    22021 tctgttatag gttcctgcat tagcactctc catgaagtgt ttatttatgt atgtatggag
    22081 gattaatcta tgcgcatgga taagctgacg ttaaagtcgc agaatgcgtt atatgaagca
    22141 caaaatattg caacgcaata taaccatcct gaaatacaac ctgagcattt attgtatgca
    22201 ctggtaaatc aggaagatgg tatattcagg cccatagctc aaaaattggg tgctggtgtt
    22261 gatgatatca aacatgaggt aaccattgct cttcagtcat tgccaaagca gtatggtgcc
    22321 ggccctggtg gtggtaccat gtcgggcggt ttgcgcgatg tattaaacaa tgcctggaat
    22381 gaagcggttg ccctgaaaga tgaatactta agtacggagc atcttatcct gggacttgcc
    22441 acaaccggta aaaacaaggc acgcgatatt ttgaattcac acggttttac taaagaaaat
    22501 attttgcgtg tgcttgctga catacgtggc aataaacgcg tgaccgatga aaatgctgag
    22561 gacaaataca acgcgcttga acgctatggc agggatctga ccaaactggc acgggcaaac
    22621 aagcttgacc ctgttattgg cagggatgaa gaaattcgcc gtgtgatgca ggtgctatcg
    22681 cgcaggacaa agaataaccc cgtgctcatt ggtgagcccg gtgtaggtaa aactgccatt
    22741 gttgaaggcc ttgcccagcg tattgttgct ggagatgttc ctgaaagcct taagaacaag
    22801 cgtgttgttg ctcttgatat gggagcactt atcgctggtg caaaataccg tggcgagttt
    22861 gaagagcgct tgaaggctgt gattcaggaa gtggaagagt cggaaggcga tgtgattctt
    22921 tttattgatg agcttcacgt gttagttggt gccggtgcgg ccgaaggttc ggtggatgca
    22981 tccaatatgc ttaaacctgc actggctcgt ggtgaactgc gctgtatagg tgcaacgaca
    23041 cttaacgaat accagaaata tattgaaaag gacaaggcac tggagcgccg ttttcaacca
    23101 gtgtttgttg gccagccaac agtagaggat actatcgcca tattacgtgg attaaaggag
    23161 cgctatgagg ttcatcatgg tgtaaggatt tcagattccg cagttgttgc tgctgctgta
    23221 ctttcaaacc gctacatcac cgaccgtttt ttgcccgaca aagctgttga cctcattgat
    23281 gaggccgctt cactcattaa gatggaaatt gattcaatgc ccatagagct tgatgaaatt
    23341 gaacgaaaaa ttcggcaact tactattgaa cgcgaagccg tgaaaaaaga aaaggataaa
    23401 gcctcacttg aacggcttgc aaagattgag gaagatctgg ctaatctcaa tgaaaagaag
    23461 aaggagcttt cggtacagtg gcaaaatgaa aagaacatca ttttggaaat acgcaaaatt
    23521 aaggaagaga ttgaaaacct gcgtgctcag gaacagaagg ctgaacgtga gggcaatctt
    23581 aacctggttg ctgaaatacg gtatggtaaa attattgagc acgagaagaa gctaaaagct
    23641 cttaatgata agctgacaca gattcagaaa gagcgcaaac tgctgaaaga ggaagttacg
    23701 gagcaggaga tagctgccgt tgtttcacgc tggaccggaa taccggtaag caagatgctt
    23761 gaaggtgaaa aagaaaagct cctgaaaatg gaagaaatta ttgaaaagcg tgtggttggc
    23821 caggagcagg cagtacagac aatagctgat gcggtacgcc gcagccgttc aggattgcag
    23881 gatcctaaca ggccaattgg ttcattcgta ttcttaggac caacgggtgt aggtaaaacc
    23941 gagacggcaa aagccatagc tgaattttta tttaacgatg aaaaggccat agtacgcatt
    24001 gacatgtctg agtatatgga aaaacatgcg gttgcgcgcc ttattggtgc accacctggt
    24061 tacgttggct atgaggaagg tggtcagtta accgaggcaa tacgacgaag accatactca
    24121 gtggtgctgt ttgatgaaat tgagaaagct caccctgatg tattcaatat ctttttacag
    24181 atacttgatg aagggcgact gaccgactca aaagggcgca cggttgattt taaaaatacg
    24241 ctgattatca tgacatctaa cattgggaca gcgtatatat cagatgcatc aataccttat
    24301 gaacagcggg taaaaggcgt gcaacaggag ttgaagcagc actttaagcc tgagttttta
    24361 aacaggattg atgacatcat aatattcaac ccgttaaccc gtgagcacat caacaagatt
    24421 attatgatac agcttaatga agttggtaaa cggttaaaag agcgcggcat aagccttgaa
    24481 ttaacaccaa aagcactgga ctaccttgtt gaagttggct ttgatccgca gtttggtgca
    24541 cgaccgctta agcgtgcaat gcaacggcat ttactcaacc cgctttcagt taagatttta
    24601 gctggcgaag tgatggatga tgatactgtg cttgttgact ttaaaaacgg ccagattcaa
    24661 tttaatgtta aacgaagcaa gcaggaaaaa aaggttgtgt aacaaaaaca tttgcgcacc
    24721 gtttgaaggc ggtgcgcaaa cgtcagtaac attatttaat ttagttattt aaaatacaat
    24781 acctgccatt gcataccata taggtgccca ggttacatct gcatcatcgg tacctccggt
    24841 tggctgggct aaaaatccaa aggtaagccc cagtaaatat ccgccatcaa gcgcaaactg
    24901 aagctcagct gagaaattaa gagcaagacc atctgcactt gtatcaatcc ctggctgaga
    24961 taaatctaca gttgtacttc ggtatcctat ttcaaggcct ggttttatag ctgcagttgg
    25021 agatatcatg aacttgggtt taaatgtaag accaaattca tacacgtcaa catcaacact
    25081 tggatttact atctctggag aatctgttga tgttgctgca tactgtccat atacaccaac
    25141 tgaaaacatg ggggaaacaa actgatcaaa ataggctttc agggtaaagc ccatctcagt
    25201 atccacgtca gcatcttcaa tgctaatagt gcccggtaac aatacaccac cataaagccc
    25261 aatgtctctt gtcctgtttt caagattttc tgcagccatt ccaaacgata ccattgttaa
    25321 gcagacaagc acggcaaaga taagttttac agtgtacttc ataaaaacct ccacaaaatt
    25381 aattttttaa aattttaata atcttttata gtcattgaaa tgattataaa aggaatatcc
    25441 aaaatactat aactgtgtgc aatagcctat gtttataaca taggttttat atcctgcatt
    25501 atcgccgctt tcacttacct gctgcatcag ggaactcagt gaaagtccca gtggtacata
    25561 tacaatatca aaaccaaaat agcggcttgt tattgttggt gcagtaactg agatagaacc
    25621 agagccctga ccttcaatat cataatccat atcaacattg ccggattgcc gggtaatcat
    25681 gacaaatgga cttatggtga agtcctgtaa tttgaaactt atctgtagtc caccctgggg
    25741 tcctttcatg gttgtggtta tgtcaacagt tccttcataa cctggtaaat ttgaatctat
    25801 tccaatgtaa tttcgcgaaa atccaaaacc aataaagcca ataagtgata tctggtcatt
    25861 attaactaac agtatttcta aatccagtgg taaggaagca agccacatgc tcatatccgc
    25921 ttcattgcca acgtcgccgg ttgctccaac cagcatcagc ccaacatcaa atgccaggat
    25981 atctttaaat gcataccggt aaataccgcc agcaccagca ccccatacct taacatcgcc
    26041 ggtgagtggt ggtgtgctat caccagtgat gtacatcccg gttgctgtaa cacgtaagtc
    26101 tttctggcct tctttgaact caaaccatgg aataggtggt ggggatatca tgaagttatt
    26161 ctgctgagca acaacaggaa tggaaataaa gattaacaaa acaagacttg caaaacatag
    26221 tacccttttc atggcaaacc tccactgtaa tatatttgtt aaatagatag gggtaaatta
    26281 agccatggtg gttttattat attatgcaag ttttggtaag gtagatgatt ttaacattta
    26341 ataattgaaa atatttttaa acgacagcaa cctgcacaac taataattga tttattttga
    26401 aaaatgcctg actgcttgaa aaactaaagc tatatgtgca ctggttcaat ccactttatg
    26461 gttacaccac atttatttaa gtatgtgtta tgtataatgt ttatgtcaag caaattttac
    26521 aaattttaaa ataattgaca tcattctaaa gcatagtata ttttcaacag tagtaagagc
    26581 aatctactga cactacaagg tgatgccatg gactatgaaa tctatgatgt gaacaatatt
    26641 ctgcttccgg tggagcataa gattggtgcc ttaaaccgtg caaagaatct ggttgccgag
    26701 atgacgcatc ccaatattga ctggaagtac atggtcaccg aactcagagc ctacctgtat
    26761 gattacatgt atgatatagt tcctcacagt gataaagttt tgccggttat ctttcattac
    26821 ctgaaagaag caacggtacg aaaacgtggt tcaacgctgc gtgctgcgga tacctttctg
    26881 gatcgctatc tctttttaat taaaaaagaa attgagggcg atagttccct ggaaaatgtc
    26941 actgccatgt ttgacaatga gtcaatacat ttttgccaga tattaattgc cgacactgct
    27001 gatgggtttt acctggaaga tgttaatctc aggattttgc aattattaga actatcgctt
    27061 aaaagaaaaa aagtagatga aacccttttt gaactgtgca ctgaaattat aataaaccag
    27121 tttaaattgt atgttgaacg cagtatcata gttgacgatg aagaagtgta ttcactgcaa
    27181 aacctgtgga gcattgagca tgaacacata cttaagcttg agcagcttgt taaatcagtt
    27241 actaaaaaag cgtatcagga aaaattaata aaagctaatg ctttaaaaaa cagtaaaaaa
    27301 gacagggcaa cactccttgc tgaaataaaa gagttgattg attttcacca caataccacc
    27361 tcatgggaaa agatctgtat tgctgcaaaa gagtgcattg ctcagaatgt catagaatat
    27421 gatgatgtgg tgctggcact cttaacgttt ctggttaaaa aatcgcagga gggcagggat
    27481 gcaaatcttc agctgtatat atcacgaagt gttgcatcgt tatgcagcgt gatggtacag
    27541 cagcaacgtt ttgtgctact acgacaggtt gttcagatgg ttgtacctgt gcttgttgcc
    27601 gagattgagc gtggtggcaa ttataatgct gcgtttgcaa ctatcctgaa tattggcaaa
    27661 acggttgttc aatccgataa ccggcagatt attgaccttt ttgtcgatat tctggtacat
    27721 gcaaaattct gctttccaca atttaccggc attgctcagg attggtcggt tatggtcaat
    27781 gcaagccacc ttgcaaatat ccgtacgtgg cttgagctta ttgagcttaa cccggtgtat
    27841 atgaagcgcc tggctgcaag ccttatagcc aatttaacgt tggggggtgt ctttttaaaa
    27901 gatacggatg tatttcagcg tgatatttca cgccttttaa acagcaacta taaagatgta
    27961 ttttacctta tcatatcgct tgcggcagtg ttccctgcat tctaccatga tattggtgct
    28021 accggcaaca tccgtgcgtt taccgagcgt gttgacacca atcaccagat gaatgacctc
    28081 atacattttg tgcgcaagca ggtgcatgtg gaatcaagca gccgtacggt ggtgctgtta
    28141 cagcgcgtga tggacttctg gcttaccggc gacaaagagc tattaaaagg tatggtgcca
    28201 caggaagtat ataacaacct tgagcgcaca tttcgtctta ttaatttaga caatgagtca
    28261 gtggcacgaa gaatatatac cgaaatacgg cactatttcc ctgaactggt atatgaaaaa
    28321 ttctgggatt tcttctacaa agttggcaaa aagcgtttta tggatgtcat tgcgcagcat
    28381 acatttgaag gaatggatga ggatgaaaaa aaggacgcta ttgagtgtat agtagaatat
    28441 tttgacaaac agttcccggc ggagatgacc aaaatgctcc accatattgc cggtatgttt
    28501 gatattgata cctcacgcag gcagatatgg cgatttttat atgagatacc ggatgatgac
    28561 ttcaggaaga tgtttgaaaa tgtacagaag ttagatgttt caaatgtcaa tatagagaaa
    28621 tttattacgt tccttcatgt gtacaggatg atctttgata aatacaattt ttctgatata
    28681 cgagccatag aaaagcttca tcagtatgca caggaaaatc tttttagtcc accagaaaac
    28741 tttttcaagc gaattgaagg caatgatgat tttgacgcac tcgaagccat tctggaactg
    28801 caacatacgt taaaaagtga tattttgcta tcccaacagg tatttgagcc agttgatacc
    28861 attgagttta aacgccatat tgcgtttggc ataccgtcaa tgtatggctc atataaagaa
    28921 aagaagtttg atacactgaa agtgttcttt cactgcaata tagtgaggct cctccttttt
    28981 gaaaagatac tggaaaatat cagcatttat ccacatcaaa aagttgacta tgatgcaatc
    29041 aaacgcgtta ttaaattgtt catacaatct tttgaaattg atggccttgc aaatcatgag
    29101 atgagggctg tgacaagcct gcttgatgca ccaaatttga cattaacaca gtttcgtgat
    29161 gttatttata gcctgctggt tatccatggt gaaatttcgg acaggtttaa tgatacgttt
    29221 aaatcagtaa gccgtattgc aattaaaaat attggcattg ataacataat ccatgatttt
    29281 ataccaccgg atcaacctgc aagtattgaa gttattgtgg acaggttttt acgtaacagg
    29341 gtgatgcaat ccccattgtt gcagctttta gacaatctat tgctcaagct aaaagacaat
    29401 cttattcatg aactgtcata tttgggcaat gtggttattc tcaataaagt ggatacacgc
    29461 atacacaaag gcagacttgt ccacataatt gggaaataca gcgtgcaaca tgacgaaact
    29521 gaactatttg ccccactgtg ggaagttggt gccaaagctc aggggttaat tattgcggca
    29581 aatattgatg gcattaatgt acctgaaggt cttgtcatct cttcagaatt atataaacgt
    29641 attaaagatg gcaatatcaa taacccgcgc tttaagcgaa agctcatata tatgttaaaa
    29701 aaatatattg atgaatttac tggctatcga tttggaaatc ctgaaaatcc attacttgtc
    29761 tcagtgcgta gtggtgcagt gttttcaatg ccaggtgtta tggatactat caccaatgtg
    29821 ggcatgaccg aggacattgt accctatttt gcgcagtatg atgagtggtt tgcgtgggat
    29881 tgttaccgac gtgtaattca tgattttgca atatcagcat ttggtatgga caggcacatc
    29941 tttgaaaatc ttatggcaca ggcaaaagag gaagctggcg ttgatcttaa agaaaaactt
    30001 aatggcaagc agatgtcatt gctcacgcgt aaatatcgct ttgccattaa taaagcaggt
    30061 tattctgtgc caaaagaccc ctatgaacaa ttattttatg caatcattgc ggtatttcaa
    30121 tcatgggatt cggccattgc ccagaactat cgccgcttca taaacttatc cgatgactgg
    30181 ggaacagctg ttattgtaca gcggatggtt tttggaaatc tttcaccaac atctataacc
    30241 ggtgttgttc atagtcagta tattgaatat gaggatgttc agatagcggg cgaatataaa
    30301 acccgcgccc aggggcatga tattgtgagt ggggttgcaa aggtattccc catcagcgag
    30361 cagcaaaaat taaaatttgc acgctttgcc ctgtatccat ccatggaaaa ggcatatcca
    30421 ggacattatg caacattacg tgatgccgtt acgcgattgc gcaaacgctg ggataatgac
    30481 attgaggtag agtttacgtt tgaaaacgaa aagctgtata tactgcaaat ccgtggtatg
    30541 gccaaacata tgtttgatat agaagaactg gttgagacac cacaagagct tcaggagcat
    30601 ctgttagggc aggggctggc agcttcaggt ggggcagtat caggaagagc agtgtttgat
    30661 ataaaccgca tagagataat gcgcatgcgc tatccaaagg ataagataat tttaatacgc
    30721 ccagagacca acccggaaga tgtgatagga ctacagaaat cagatggaat attgacatct
    30781 gtaggaggga tgacatcgca tgcggtgtta cagatgcgca gacttgaaaa atcaggtgtc
    30841 agcgattttt ccataatgaa aatagtagaa gatgaaaata tagcaattat aaaccgtgaa
    30901 cagggtggca aaataattat ccgtgaaggt gattttataa caatagatgg taataccggc
    30961 catgtgtatt tagggtttca tcctacccgt aaagcccacc ggtaaacgta ccgtatataa
    31021 aaaagcttga aaaattaaaa ctaacttttt caatggacgt aatgagtata aattttacat
    31081 tagtttgatc agtaattact ttaatttata tatagaaaca ctgggtatgg gattaggtcc
    31141 tgcttgatat aataacacaa aacaagcata tggaggatta ttatggcagt atcaccagat
    31201 gcacctattg gtgtaatggg cattggccga attggaaagc ttttggtgtg gctgtatgct
    31261 gcaaaaaagc agcataaaga gattgtcatt gcaaccggca gaaaaacggg tacatcgtta
    31321 gatgatttag caacctattt tgaatatgat tcaacctatg gcacctttga tgcgtttatt
    31381 ggtggattta acagaaaaaa tgcggttgca gttaaggacg ggcatgtata cctcaatggt
    31441 gtgaagctga tatggttgaa tgagcctgaa tttcgtgtgc cgggcaatat accctggaat
    31501 aaatacggcg ttgacgtggt ttttgacacc accggtaaga tgcttgatcc aacgcgcaat
    31561 gatgacaatt cgctcagggg gcatctaaac cacgcaaaga aggttatact tactgcaccg
    31621 tttaaagtaa aagataaagg caaacaaatt cctgaggatt caataaccgt agttggcgga
    31681 gttaactttg acaaatatga tggcaacaaa catgccataa tttccaatgc atcgtgtact
    31741 accaattgtt gcgcaccacc aataaaagcc ctggttgacc attttggcga caagtttatc
    31801 tcatatgcgc tgactactgt ccatgcggta accaattcac agtcaacgct tgatgtgctg
    31861 ccaaaagatg gcgataagga cacccgcaaa cgccgcagca tattaaacaa catgatacca
    31921 acaagcaccg gtgctgctaa agcagtgctg gaagtcatcc cggaagtaca ggctttgggt
    31981 attggttctc aggcgtcttc aatacgtgtt ccggtgaata ctggttctat tgttatactg
    32041 gatgtttcac tgttaggtga ttatgacaat aaatacatcc ataacatatt caggcaatat
    32101 tcagaaaaga atcctgatat tatgtattac tctgaaaagc agcttgtcag ttcagatatc
    32161 ataggcagta cctattccac tatctatgat gctgaattta ctcatcaccg tacaaccaaa
    32221 cgaggcgata attactacac gatggttgac ctcaatttct ggtatgacaa tgaatttggg
    32281 tatgtatgtt cactatcacg cctttatgac cttgttacgg gcaggcgata gttactgtaa
    32341 attaacctta ttctattaaa aagggcggct aaaatcagtc agcccttttt tatgattgct
    32401 gcagttaaaa aaatataatt gacagcataa tataatataa ttatatttat aaaataagga
    32461 atagatttat gaaagtatgt tatccactaa taatactgtt aatattaatt atttttttcg
    32521 cgggttctac atatgcaaag gatgtccctt atacattaga tgatagagat cgtataatac
    32581 gagtggaagt aaaaataaat gaaatggaca aacgctttga gcagatagat aaacgctttg
    32641 aacagataga taaacgattt gaccagatta ttactttttt atggatattg acagcgattt
    32701 ttattacaat aacttcagca accctgggat ttgctttctg ggacaggaaa actgttttaa
    32761 aacctataga agaaaaagtt atctcaaatg aaaagaaaat tgacagtgcg cttgctgcat
    32821 tcagaaaatt ggcaactgtt gataagaagg tatatgcaat attgaaagag tttaatttgt
    32881 tataattata ttaatgtgtt cttaagctct gacccctttt cttatttttt ttacacccaa
    32941 tccaccacat acgaatactt tttccgataa tactcctcat accgcataaa ctttgctacc
    33001 ctttcagcaa acgtcttccc caactgcaca aagtaatcat ggtgatctat aggtactcct
    33061 acatcagcat
//
LOCUS       DAORYL010000027        32696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1129_length_32696_cov_11.266352, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000027 DAORYL010000000
VERSION     DAORYL010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 32696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32696
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1129_length_32696_cov_11.266352"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            323..1510
                     /locus_tag="PLV81_06850"
     CDS             323..1510
                     /locus_tag="PLV81_06850"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOM09855.1"
                     /translation="MNYPLKNLKILDFTYLLPGPYGTMMLADLGADIIKVENPNNPDM
                     LRMLPPVVNGISAVYAHINRGKKSLALDLKSQEAKDIIYTLVKDYDIITEQFRPKVLE
                     KLGFGYNDLKKANPSIIYCSLTGYGQTGTYALRAGHDINYMALSGVDSFSGKKTTGPS
                     LHGIQIADIASGAKNMVIGVLAAYIKRQATGEGDYIDISITDGVFAMSAFTTAAYIEG
                     EPLPQPETQMLNGGGIYDYYITSDGRYLSVGPIEPKFFQNFCQAIGYNDILQNVDFSP
                     EAVYLHKKKIAAIIASKPLSYWIEVFSNIDACVEPVRTLEEAINNPPLSERDMIVSIT
                     NNNGTLYKQIGNPIKFLSHNYIAQKAGVPLGYNNDEILKSIGYNEHQIQQLYAKNVIA
                     KFN"
     gene            1519..2835
                     /gene="miaB"
                     /locus_tag="PLV81_06855"
     CDS             1519..2835
                     /gene="miaB"
                     /locus_tag="PLV81_06855"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOM09856.1"
                     /translation="MKKPKTFTIVTFGCQMNKSDSELMEVSLVQEGFVKSSSYGDIIL
                     FNTCSVRKHAEDRALAHIIEARNACKQSIIVVAGCMAQRLSFELRSNYGVDLVVGPYH
                     SPSIGSIIKNYIQNQNISEFTSQDIKDFASRIHPSLVNHKDELPWHKWVTISHGCENY
                     CAYCIVPYVRGKLISFPSQQIIDYCKLLIDNGITEITLLGQNVNQYGQDSGDIPFYKL
                     LENIARIAGLYKLNFLTSHPKDFTIEILHVIQDNPVIARSIHLPLQSGSDSILQAMNR
                     QYTMKHYYAIIEAIQKLLPMASISTDLIVGFPGETQQDFEDTLNAVRTIQFDEAFTYK
                     YSPREGTKAFTLPDSVPDTIKQERLDILIQTVREISQRRLMNHIHTTTDIIVERISKK
                     SENEVMGKTICNHPVIIKGGKEDIGKKYTVTIVSLKGSTLYGERSA"
     gene            2828..3940
                     /locus_tag="PLV81_06860"
     CDS             2828..3940
                     /locus_tag="PLV81_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09857.1"
                     /translation="MLKTICIVALCTTTALAGEQSDFEAIQKAFKLNSSNKQELVKKY
                     FKTYPNSNYIPDIRMLLAESASTVDGSIAEYHKLLNNYRYYAKRDVAFLSLCQLYYIK
                     SDWGNLLTICNKAIAECSTSNYRPIFLQLRAIAHYHHNHYDDGIKDCEKIPEYSRQLN
                     TLAYSLFLKSEMDRQMYGYARQYIKGLAELLHGYTEAALYPTCLLQLGQYYEYNKMYN
                     EAWSTYNFLMSQFPKSPEAVKANTYAQNVRKYNPKTVAFMPDEKIIAQSEAIDIKPDV
                     EESDTQQNLKLYYTISIGPFYNLQKAKELQKLIVNYVVPVHIVRKEKSFDIYAGHFPS
                     LDEGMSIKIQLAEELGINGFIIEIFEQDGKQYIYGQ"
     gene            3930..6491
                     /gene="mutS"
                     /locus_tag="PLV81_06865"
     CDS             3930..6491
                     /gene="mutS"
                     /locus_tag="PLV81_06865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOM09858.1"
                     /translation="MGNDKELTPMMRQYLEIKQHYKDEILFFRLGDFYEMFFDDAKIA
                     SKVLDIALTSRQNDIPMCGVPYHAADSYIARLIKAGYRVAICEQMEPPSSSTIVKREV
                     IRVITPGTVVESNLIASDENNYLGSVVIHNNTVCTGFVDVSTGDFFLSSADYSREFIA
                     TEISRFSPKEMLLYGSQQELTFLHDLLKQQDIALASINEWYYDVEYLTNIICNIYGLS
                     NIKGLGLTHTAILTVGSLLHYLKDTHKSALVHLKYPKNVSRSEFMVLDHQTIHNLEIL
                     KSQDGSKLHSLFGVLNHTQTAMGKRTLERALLYPLLDASALESRYDIIAYLIDNVQLL
                     KEIQEKLSRIHDLERIVSRFALKKYYNRDFIALQQSIEAASDVYAVLQQHPDNLFNML
                     GTISDSLQELSLKIKNTIVDNPPLSSEHGRVIKEGVNAELDRLHTLKTDARQWLLNYQ
                     EEEKRRLDIPTLKVRYNKVIGYYIEVSKGQTGKVPQDYFRKQTLINAERYTTDTLQKF
                     ETDVLQAHDSIIKIETEILESIVETILANKLQIQELAYTIGILDMFCSLALAAYQNNY
                     IRPMLNNDGIIDIKAGRHPVVEKFYIKEPFVPNDIFLDTVENVIAIITGPNMSGKSTY
                     IRMAAVLQLMAQIGSFIPAQLANLCIVDRIFTRIGASDNIARGESTFLVEMNETAIIL
                     NNATDKSLIIMDEIGRGTSTYDGMSIAWAVVEYIANYIKAKTLFATHYHELTKINKKG
                     IVNYTVMVKESISGVEFLHKVVPGAADKSYGIHVAKLAGIPKPVTVKANKILEKLEKS
                     SRIDSLIKAPDNEEQLEIFQATHHRLAQALKDITVENLTPLEALNEIARLKRLIE"
     gene            complement(6493..7254)
                     /locus_tag="PLV81_06870"
     CDS             complement(6493..7254)
                     /locus_tag="PLV81_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOM09859.1"
                     /translation="MFQALPTLIILLVLLAVSAIKIIREYERAVVFRLGRLLQPPTGP
                     KGPGLVIIIPFVDKMIRVSLRTVAMDVPPQDVITRDNVSVKVNAVIYFRVIEPNKAIT
                     EVEDYLYATSQLAQTTLRSILGMVELDGLLSDRERINHELQKILDKQTDPWGIKVTAV
                     EIKHVDLPQEMQRAMAKQAEAERERRAKVINAEGEYQAASKMVEAAKLMEPHPIALQL
                     RYLQTLREVATEKNSTTLFPIPIDLVEPFLAGRKK"
     gene            complement(7333..8628)
                     /locus_tag="PLV81_06875"
     CDS             complement(7333..8628)
                     /locus_tag="PLV81_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HOM09860.1"
                     /translation="MKVRLLTSMLLIIAFSSTVFGADKYALIELNGSVNPVIADYIVN
                     SIKKANQDNMQFVLMIMDTPGGLLNSMRDIIKAILDSEIPVIVYTYPKGAQAASAGGF
                     IMIAAHIAAMSPGTEIGAMHPVSPFLNFSPDQKQNGIMEKKVLNDTVAYAKSLAELRK
                     RNITWVEKAVREAISSTNNEALRLGVIDVVANDINDLLEKIDGKKIKTKDIYVVLKTK
                     NIQQHQYTMDWKEKILNYFADPQMVFFLFLIAVVGIGFELKNPGMIVPGVIGAIALFL
                     FLLAVKVLPINFAGLTFIILAIILFVLELKFTSYGLLTLGGIISFIIGSMILFDSPLP
                     GGQIPLTSIITVLIVLLLFIFVVVRAVIKVHTQKAVTGIEGMIGEKGEATIDFTGKGT
                     VEVHGELWTAISDEPVKKGDVVEVIKMEGMVLYVKKVSQ"
     gene            8894..10096
                     /locus_tag="PLV81_06880"
     CDS             8894..10096
                     /locus_tag="PLV81_06880"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOM09861.1"
                     /translation="MRKICFIVIIMLTSLSIIPGQQLEKKNMDVVGDSSQNKSIEDNT
                     QSLTAESPTTQSAKDEKKDINNTSQEKKKITEEKQKDISQDKKIEAEKRKTEWIKKTI
                     QYGIQDDRIDAIKSTLTIKDPSQKNDIIKLLDELMPQETNPEVKRTAITVVSELKSSS
                     SSSWIIASLNDNSEEVKIAAVYAIDTLKATEAIPKLEGILKEQKFTENSNFTEALIRT
                     LGKLKDTQLKDFAIERIKDTKTTDNLRQLFILFLGDLEDISSKDFLLGIVKDKDEDTT
                     IRSYAAVALAKIGAKDTAGDLLEIIKEIDSYPVNKRKDYYSFSMYCTAALVKMGDSNA
                     IPRLMEALRSNNPVVRLRAVELMKDLKDKRTIDILKYKIQYDPSPKVQKAAKEALKEM
                     GENVDESK"
     gene            10492..11427
                     /locus_tag="PLV81_06885"
     CDS             10492..11427
                     /locus_tag="PLV81_06885"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOM09862.1"
                     /translation="MALVSDFDKYFLHLPKISQPDDFEKFWQSAINELKKTPIDVQYV
                     HNKRKSTGKFSAYDMTYSGYGKIQLFATLYIPDKITRPHVVILFHDYDDLESYAQFPI
                     DLPFAYCFVELRGHRNIIHYDQEQNKYPGFMTEGILEKDEYYVKGLYLDGLRTIDALR
                     LHNDLDCSKIAIIGKGLGSAVALFTTSNCNRIVALVLDTPSFCYLDKAQNIAKSVIAN
                     EINSFIDEHRSKKSTIKKNLNYFDALQFINAVTCPVLTIVGFKDSLSPAECVFALFNH
                     LQCDKTIEVYPEEGHGAGGIEQQKRMIEWIKNIMV"
     gene            complement(11472..13046)
                     /gene="murJ"
                     /locus_tag="PLV81_06890"
     CDS             complement(11472..13046)
                     /gene="murJ"
                     /locus_tag="PLV81_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOM09863.1"
                     /translation="MKTESKDKSLFNTVMVATGIFLSRIAGLIRDRVFAHYFGNSDAA
                     DAFRAAFRIPNFLQNLFGEGVLSASFIPVYAKLLAEKKFDQATKLARSIAGILSLVIS
                     SLVFVGILITPLLIDIIAPGFQGEKRELTILLVRIFFPGAGILVFSAWCLGILNSHGK
                     FFLSYSAPVFWNLIIIITLLFFGDSSTQNDLATYTAAGSVVGSLMQVLIQLPFVLTIV
                     KNVTLRFDTQSREVRTVIKNFIPVFFSRGVVQVSAYVDSLLASLLPTGAVAALSYAQT
                     LYMLPISLFGMSVSAAELPSMSKVIGPQEEIASKLRNRINNSIKRIAFYIVPSTVAFF
                     ALGDILVGAIYQTGKFTTHDTFFVWMVLAGSTIGLLAITQSRLYSSAYYAMNDTRTPL
                     KFAIIRVLLTSVTGYYASQYGPLLFHIDMVYGTVGLTITAGLSGWVEYKMLQVSISRK
                     IGTVGLEKKYLFYLWTSALIAATIAFFIKINLHYHPLILAVTLLPLYGVLYFFFAFIF
                     RIEEIMNILKMVGFKK"
     gene            13334..15301
                     /locus_tag="PLV81_06895"
     CDS             13334..15301
                     /locus_tag="PLV81_06895"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOM09864.1"
                     /translation="MKKIIIYTIIMVTIASMSVAGDWPIYKGNLFFTGNNDEIIVSNN
                     QLKWLFLAKDRVFNPVVSDGRIYFVDKNAFLYCIDEDSGNIIWLNDVRSISSQFKAYS
                     KSAGKIKYPLVYGNTVIVSDPIAIYAFDKRTGNVIWARTGLRVDETKPQGLTGITTQI
                     SIDGIYADPIIDQDTIYYGTRKIFASRDIKNGHIIWENKDIATYSGFPVYYDQYIITQ
                     SMNYETKTFTVFCLEQRTGKVVWQRNFNQPVTILPPVVNKHVYVPINNAVYCLDIKNG
                     ETVWQKSYNGTITSPLSFTNRSILFSLDNASIVGINPENGSTIANIFVGQQSSPYFVT
                     IRDVIYIVYNQHNDKNQPVAKVKAMYINSGNIWWEFTAPFPGGVSQPVASDGILFLPA
                     GNYLYAIGAGYMGSQSYESSKPEETKKGEPQEPSKLPDQKLSEKELADKTITPESKPA
                     PQPAKQEMPTRKLPVEITDNNKAPLKSRITIQYKDENGEYNQSTAVNGKGDITIPDKD
                     NVEVIFEADGHVPKKVIVNKSDSQLKIVLDEIQPGKAVVVDNIHFDFDKWYIRKDSIN
                     ILDKLVDMMKANPYLKVEVWGHTDSIGDDTYNQKLSEKRADAVCDYIIKHGISPERIS
                     AKGFGETKPIASNDTKEGRQKNRRTEFYFKS"
     gene            complement(15286..17283)
                     /gene="recJ"
                     /locus_tag="PLV81_06900"
     CDS             complement(15286..17283)
                     /gene="recJ"
                     /locus_tag="PLV81_06900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOM09865.1"
                     /translation="MCTSASSYVKDLLLSHFNISHRVADLLLLRGIDSPYSAYTFLNP
                     DLTLCYSPFLMNEMDEAVRIIKNAVSNKKKIGIFADSDIDGLTSLTLLTHLLDLLNVK
                     YFYRYPVGDELYGLTISIIEEFKKNNIDCIITLDSGTRDVNEIAYARSLGMDVIVCDH
                     HEPSETLPDAAIINPKLMTSQYPFKELAGVGVTYKLCLAVLLSYTKYYDKYIIVIALS
                     HDSILYKVINRGRAIAHGSASSIQEIKHLSKQYTGAILFNYDVPDAALLQHGMHYDIR
                     DFVDELSPIHQIIKGDIKDLFPSPVDYATMLLFKIQYQQSKKISQFIQDMLPLVALGT
                     IADMMPLIDENRIIVSNGISYFDKTNHCGLKVIREYIAKTINSDTIGWDISPLLNTPG
                     RFGKTKLAADFLLEKKPSKVKELLQEIIQLNKERKTIIQTLVSKFSNDDSNENHALRV
                     ICSDEIPDGLTGIIANRLADQLLQPVLVISDKPDAEIIKGSGRSRNNFDFFTSVSKYE
                     HLFEKLGGHNHAFGFSIKKENIPILLKELDHTLCYEATENNIPEYDYELLVEDITFEL
                     LEELKLFEPYGIGHKPFIFLSKNCTISQYFIINGKHCKLIINGSIKLEAMAWNSADKY
                     NDILTQKIPVDILFTVKANEFNGLISPLLIIENLYPSQDLK"
     gene            complement(17305..17973)
                     /locus_tag="PLV81_06905"
     CDS             complement(17305..17973)
                     /locus_tag="PLV81_06905"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HOM09866.1"
                     /translation="MLLSIDRVLQLLAEGKSLEKIAELSDVDVSEIQSVIEEARKIIL
                     EYDKQRAKSKIIVKKKSSITENKNQAEVSDNIFEGLEVLSTIPTESVLTMYIDGASKG
                     NPGPAGIGIVIYDGDDRLVGKVSAYIGKKTNNQAEYTALLRAIQLALYFKTRTLKIRS
                     DSELVVRQLSGEYQVSNPNIKKLYERVLELKKQIPNIKIEHVMRNFNEKADFLAKKAA
                     EKKR"
     gene            complement(18037..18735)
                     /locus_tag="PLV81_06910"
     CDS             complement(18037..18735)
                     /locus_tag="PLV81_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09867.1"
                     /translation="MSPEIYTMLELQSIWDKVLAAKNEITRSRHAIDFWRNEMKAMEA
                     KMADLRSRVNQLKTNIKQKELELHDVEARIQFLDSRKNSLKSQREFDSMITELETLNQ
                     KKDEIENSMLQYMEDVDAIENELSVLQQEMPKRLNQIENDVEELQNKLELLQKEAEDN
                     ASQFNKQMTELPPDLRARFAKVIETKGNGIVPLENGICMGCHFQVPVYVKDEVAKSKA
                     VTCTNCGRFLYFKN"
     gene            complement(18737..19540)
                     /locus_tag="PLV81_06915"
     CDS             complement(18737..19540)
                     /locus_tag="PLV81_06915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOM09868.1"
                     /translation="MHSIKGLFDYLEKVFPLEYQEEYDNSGRQISFDNKPIKGILVAM
                     DVSPVVINEAHENNCNCIITHHPIFFKPIKNILDGNSTGQMVIACIQHEISVYAAHTN
                     ADAVCWDLLAKSLGLKNIALLFPDKRFSEIGYGATGDYEYDITLSDILKKISQLLQST
                     SMIYYGDSGKPIKKVACLQGAGSRKINAVISHGSVDCIITGDVGYHDAVFANNNGVAV
                     IDIGHYVSEKPIIDFLYQTVYNYLTKEIHCNDIPLMISNKEHNPMKCGM"
     gene            complement(19533..20048)
                     /locus_tag="PLV81_06920"
     CDS             complement(19533..20048)
                     /locus_tag="PLV81_06920"
                     /inference="COORDINATES: protein motif:HMM:NF021625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate synthase family protein"
                     /protein_id="HOM09869.1"
                     /translation="MAIVNNEEKIHVVEEIQKAFSLLHAAKCGRMFLSGASNIAYTLP
                     YVTDTSDIAMFPGGIIIYNDSIFRVKDPAFGVDHPMVLFMQKITPMHYRAAINIKNEI
                     EYKINAQALGIPVYTSLNIDNLQKTNISENNRIVCVEIKQDNMLYLIGNTPTEIAETL
                     LKINKTEHTHA"
     gene            complement(20052..20834)
                     /gene="thiD"
                     /locus_tag="PLV81_06925"
                     /pseudo
     CDS             complement(20052..20834)
                     /gene="thiD"
                     /locus_tag="PLV81_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
     gene            21004..21351
                     /locus_tag="PLV81_06930"
     CDS             21004..21351
                     /locus_tag="PLV81_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09870.1"
                     /translation="MNNTPFSCKTSDRWTVIAQDTKYWRVGIYHPEFSSADQITQLEK
                     HSCPELFICANAPCGILLMVDGKEIQYIMNPNDAIMVTEYHNGFRVDPEGYFIVVEQT
                     DFTTEFIERKTTW"
     gene            21345..22184
                     /locus_tag="PLV81_06935"
     CDS             21345..22184
                     /locus_tag="PLV81_06935"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOM09871.1"
                     /translation="MVINAHTSVYCILGNPVRHSLSPAMHNAAFTHTGINAVYVAFEP
                     ASIKQAFDAIKALGIKGASITIPFKVDVLPLCDSVDTLASRIGSVNTLVNADGMIQGY
                     NTDGYGLLRSLETHSIDYHNKKVLIIGNGGSARAIAYTLARYNAHITICGRNSNNITQ
                     LANDLRKYFTNIETILIDKLTPDITAHNNIIINTTPVGMNPDISRSPIDISLLHPHNV
                     VVDIIYSPLRTQLVQNAQEKGCTVITGDYMLLFQACMQFELWTGKTAPIDIMHQALKQ
                     ALQ"
     gene            22187..23476
                     /locus_tag="PLV81_06940"
     CDS             22187..23476
                     /locus_tag="PLV81_06940"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOM09872.1"
                     /translation="MLPHYLQSLANNESHASNFTNYSPDTIVAVLNLLGNPQHKLPCI
                     HIAGTNGKGSTAHMIAAILQAAGYTVGLYTSPHLIRYNERISINGKEITDEEISEYTE
                     MLETLCNTHSYTPTFFDAFTMIAFLHFYNSVDIAIIETGLGGRLDSTNIVTPLISVIT
                     PISYDHTAILGSTLHEIAYQKAGIIKKHIPVISATQKPEAKEVLQKEASMKQSPIFFV
                     GDHIRFTIVSQNPLVFNVMVNHSAFDQNKILKNIHCSLMGDFQAANASVAIATALMLR
                     NNNWEIVPEHIYHAMSNLYIPGRCEVLSKKPLMIFDCAHNPEALEHTLSNICKQYADY
                     EKIFCTSFMKDKDVAKMLSIIQRFTSRPIFYFQIPDSRCYIPDQEIKETFSMNVFTET
                     KSLAKLLKPHIDSNSMVIFTGTFRIYPYSRTICSLLQ"
     gene            23731..24546
                     /locus_tag="PLV81_06945"
     CDS             23731..24546
                     /locus_tag="PLV81_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOM09873.1"
                     /translation="MDLSELLPKEHVITVKSNEKQLVIKELIQKLQDLGKLDNADRYY
                     TQVMHRETLENTGVGNGFAIPHVRTDSVKKLLTIFGICNEPVEYESFDNQPVKYVMLS
                     IFPTSLSTKYLYLVGMMARIFANTEKREKIDAARTPSKIYPILTKEAKLYFDSITEIN
                     KEENHIESLSGVPSSDLDLLIRLDQLYRLLDSGDKSETLTKKINELRRFIDNRSLAYY
                     ERMRQKCQNPFAILEKNTCGGCHMVIPPVEMAKIKGKKSLAVCTYCGRFLIIV"
     gene            complement(24551..25807)
                     /gene="cls"
                     /locus_tag="PLV81_06950"
     CDS             complement(24551..25807)
                     /gene="cls"
                     /locus_tag="PLV81_06950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOM09874.1"
                     /translation="MLSVRKNLVIKSKSYHKGFNTRISYDYIIKKIKNRFFLPQFLRT
                     IKKFGISAFSGGNKVTLITDGDLFFDELFKTVDSARHSINLETYIFNSDDIGWKLAKK
                     LAAKAKKDIEVNVIYDAIGCINTSALLFEYMKKMGVELIEYHPIVPWRKYFNLYVRDH
                     RKLMVVDSKIAFIGGTNIGNEYAGKKYRGKNWRDTHIKIEGPVVKDIEYFFLENWHRH
                     GGALVDISKYFPVIKPVGDTIVMALSSHSRRRIKPIYLTYLAAIANARNYIYITNAYF
                     IPDMRIVKSLIHAAKKGVDVRILLPAISDIPFVQHASRYLYKRFLKNNVRIYEYKHAV
                     LHAKTAVIDDIWATVGSSNLDRRSFTANLEVNAIILDQSFGEKMRKQFLKDIRNSEEI
                     HLENYKRRPFIQYLKEWFCYRFRYLL"
     gene            complement(25872..28337)
                     /locus_tag="PLV81_06955"
     CDS             complement(25872..28337)
                     /locus_tag="PLV81_06955"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOM09875.1"
                     /translation="MFDYTKRSRKVLEVLAQAEGRRLNSDALGPEHIMLALLKDDDSV
                     AARILKNMGVNFEILRRNIEQAIRRSGSTIILGNLPLSARFTRIIEIAKDEARKLKNN
                     YVGTEHLLLSIFRDGTCSGIDTLIRAGIDYNVIKGEVQKILGVTPEGNSQQKTNDKTK
                     TPTLDEFAIELTRLAAEDKLDPVIGRNSEIERVIRILSRKTKNNPVLIGEAGVGKTAI
                     VEGLAQRIVHHDVPEPLFNKRVLALDLASVVAGTKYRGEFEERLKRIMKEIKASDNVI
                     LFIDELHTIIGAGAAEGAIDAANILKPALARGELQCIGATTLNEYRMYIEKDAALERR
                     FQKVMVDEPSVDEAIEILFGLRERYEMHHKVRYTDEAIKKAVLYADRYIHDRYLPDKA
                     IDVIDEAGSKARLENCERPVDIEMLEREIEDLNNRKNELVKAQEYEQAASIRDTIIEK
                     KALLSQKISDWQQRINDYAVIVDEKQIATVVAQWTKIPVEKLEESESQKLLRMEQELH
                     KRIIGQDDAISVISRAIRRSRTGLRSARRPIGSFIFLGPTGVGKTELAKALAEFLFDD
                     EDALIRFDMSEFMEKHAVSRLIGAPPGYVGYEEGGQLTEKVKRRPYSVILFDEIEKAH
                     PDVYNILLQVLEEGELTDNFGSTTSFIDTVIIMTSNVGNREFQKIGKMGFNEIDPQTA
                     QFQNVDEEVKRLFSPEFLNRIDAVVHFHKLNESHIKQILDLMLNEVNEHLEEHGIELI
                     VSPKVKQYLVKRGFNEKYGARYLRRTVQNEIEDRLAVEILNGKFKDCHRVYAGIKYDT
                     IYFKPEKNGNTGKITEPEVVHHS"
     gene            complement(28344..29393)
                     /locus_tag="PLV81_06960"
     CDS             complement(28344..29393)
                     /locus_tag="PLV81_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--guanido phosphotransferase"
                     /protein_id="HOM09876.1"
                     /translation="MFEQLLSQSPFWSQVGPSADVVMTTRVRLARNLPSLPFGNKMDE
                     ADISTLESIVHQAVVTSKYFEHAQFVSLKDCTSDDRRFLRERDIITHEMELSNNSSVV
                     FDPGHQYSILINEEDHIRIQVIKPGLQMLSAFKLADEIDDELNKYISYAFTDDFGFLT
                     TCPSNVGTGLRVSAMMHLPMLTAAKNMGEVIKLVREYQAQLKGIVHDATKTVGSMYII
                     SNKATLGKSEIDIIEEIDKVISLIIDLENEARDEYYQHHKDVLEDMVWRSYGIVTFSR
                     SMSYAEAIEHLSLIRLGVVLSIIKNIELSVINDLMIHIQVSHLQKIAGRQFASSEEGD
                     AFRAAYLRKKFEDKE"
     gene            complement(29405..29929)
                     /locus_tag="PLV81_06965"
     CDS             complement(29405..29929)
                     /locus_tag="PLV81_06965"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOM09877.1"
                     /translation="MLCERCKQREATVHLTEIIRNEKTEVHLCDNCARDMGLSSHGGG
                     FSLTVSDMMSFLDAKEMTDMVDSNTCATCGWTLIDYKRTGKVGCADCYSFFKESLSSI
                     LAGYHGSTYHIGKMPLKYIEHRKSKGMIDTVRSKNVATVSLHDLEKMLEIAVQNENYE
                     EAAKLRDEIKRLKG"
     gene            30170..31390
                     /locus_tag="PLV81_06970"
     CDS             30170..31390
                     /locus_tag="PLV81_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOM09878.1"
                     /translation="MKESKKLQNLIQLDKQYLFQNYGTRLPVCFTHGKGCILFDQDGK
                     RYIDFFAGIAVNSLGYNHPVLNKALHSQVNKILHSSNWYFNKEQIEAAMLLSKTAFPG
                     KTLFTNSGTEASEAAIKLARKFGLSQSHKKYHIISFTNSFHGRTFGAMSATAQEKIHS
                     GFGPIVPGFIYVPFNDTAAFKKTVKKYKGKICAVITELIQGEGGIKIADSSFIKEIDT
                     VTKENNILFIIDEVQTGIARTGKMFAFQHYDITPDVITMAKGLGGGVPIGAIHTKDFL
                     VDYFPKGTHGTTFGGNHLATAAAKAVLETLSKETALNNIRKTGELIIKELSLMKSKYP
                     KLIVDVRGIGLHIGVELSIPGQPLVLEALQKGLVINCTAEKVIRIMPPLTISTKLVKE
                     GLLVLDSIFAGATV"
     gene            31387..32328
                     /gene="argF"
                     /locus_tag="PLV81_06975"
     CDS             31387..32328
                     /gene="argF"
                     /locus_tag="PLV81_06975"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOM09879.1"
                     /translation="MIKIPRVKSKDLLSVTDFSKEEIISIFELSKKLKDELKQGKINS
                     YLKGKTLGMIFEKTSTRTRVSFEVAMFQLGGYALFLSRDDIQLGRGETVADTAKVLSR
                     YLDGIMIRCYAHETAIELAQNSTIPVINGLTDMFHPCQALADFFTIYESNRNFKNVKL
                     AFVGDGNNVAQSLMLTAAMLGVDFALACPKGYYPKKEVVEQAFSIASKSGSVITLTSD
                     IALAVENATHLYTDVWVSMGQEKEASRRKKDFAKYKITKKILDKCAPDCKVMHCLPAH
                     RGEEITDDVIDSPQSIVLDQAENRMHVQKAILCTLMK"
     gene            complement(32341..>32696)
                     /locus_tag="PLV81_06980"
     CDS             complement(32341..>32696)
                     /locus_tag="PLV81_06980"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOM09880.1"
                     /translation="TYGHATGDEVLKTVAQSISTSLRSLDIFARYGGEEFIICLPETS
                     IIQATRVAERIRSCINDLTFHDKKNNQFKISLSLGVSKIRENSETIEDIIRDADKALY
                     EAKSQGRNRVVVFNN"
BASE COUNT        10844 a         5924 c         5394 g        10534 t
ORIGIN      
        1 aataaaataa caccactacc atacttctgg aattgtctta taagctgttt tggttgcatt
       61 gtttgtcctt atagataaat tattccttat acataaatta tattacacag tacaaaatag
      121 tattaaatat taaaaaaatc aataatataa tttaaatatg atatattttt aaaattataa
      181 tttttcataa tacacatatt attttgactt ttcaaacatc ataattccat agtataaatt
      241 atacatcaca ctcaactttt acttgactaa tatccattta tgcctataga aattgacagc
      301 gtatcataaa tatgaggtaa ctatgaatta tccattgaag aatcttaaaa tattagattt
      361 tacctatctt cttcctggtc cgtatggcac catgatgtta gctgatttag gtgctgatat
      421 tattaaagtt gaaaatccaa ataatccgga tatgttgagg atgcttccac ctgttgttaa
      481 cggtatctca gctgtttatg cgcatattaa taggggtaaa aagtcattag cactggattt
      541 aaaatctcaa gaagcaaaag atattatata cacacttgta aaagattatg acattattac
      601 ggaacagttc aggccaaagg ttctagaaaa gttaggtttt ggatataatg atttaaaaaa
      661 agcaaatcct tcaataatat attgttcatt aacaggttac ggccaaacag gtacgtatgc
      721 tctacgtgca gggcatgata ttaactatat ggcattgtca ggtgttgatt ccttttcagg
      781 gaaaaagaca actggtcctt cactccatgg cattcagatt gctgacatag ccagtggtgc
      841 aaagaatatg gtcattggtg tgttagctgc atatataaaa cggcaggcta ctggtgaggg
      901 tgattatata gacatttcta ttactgatgg tgtatttgcc atgagtgcat ttaccacagc
      961 tgcatacata gaaggtgaac cattgccaca acctgaaaca caaatgctca acggtggtgg
     1021 aatatacgat tattatataa catctgatgg gcgctatcta tcagtggggc caattgaacc
     1081 aaagtttttc cagaattttt gccaggcaat tggctataat gatatactgc aaaatgtaga
     1141 tttttcaccc gaagctgttt atttacataa aaaaaagatt gctgctatta ttgcatcaaa
     1201 accattatct tactggattg aagtattttc aaacattgac gcatgtgtag aaccagtaag
     1261 aacacttgaa gaagccataa acaatccccc gctttctgaa cgtgatatga ttgtatcaat
     1321 tacaaataat aatggcacat tgtataaaca aattgggaat cctattaagt ttctatccca
     1381 caactacatt gctcaaaaag caggtgtacc gcttggctat aataatgatg aaattttaaa
     1441 atctattggc tataatgaac atcaaattca acaactatat gctaaaaatg tgatagcaaa
     1501 atttaattaa agcatgatat gaaaaaacca aaaacattta ccatagtaac ctttggttgt
     1561 cagatgaata aaagcgattc tgagttaatg gaagtttccc tagtacagga aggttttgta
     1621 aaaagctctt cgtacggcga tattatacta tttaatacat gctcagtacg taaacatgcg
     1681 gaggacaggg ctttagctca tattattgaa gctcgcaatg cttgcaagca gtccatcata
     1741 gttgttgctg gatgcatggc acaacgttta agttttgaat tacgaagcaa ttatggcgtt
     1801 gatttagttg ttggtccgta ccactctcct tcaattggtt caatcataaa aaactatatc
     1861 cagaatcaaa acataagcga atttacatca caagatatta aggattttgc atcacgcatt
     1921 catccctcac tggttaatca taaggatgaa ttgccctggc ataagtgggt taccatatca
     1981 catggatgcg aaaattattg tgcatattgt attgtgcctt atgtacgagg taagcttata
     2041 tctttccctt cacaacagat tatagattac tgtaaactgc ttattgacaa tggcatcact
     2101 gaaattacgc ttttagggca aaacgtcaat caatacgggc aagattcagg tgatattcct
     2161 ttttataaat tactggaaaa tatcgcaagg atagctggat tgtataaatt aaacttttta
     2221 acctcacatc caaaagattt tactatagaa atccttcatg ttatacaaga taatccagtc
     2281 attgcccgaa gcatacatct tcccctgcaa agcggctctg actctatatt acaggcaatg
     2341 aaccgccaat ataccatgaa gcactactac gcaataatag aagcaataca aaagttactc
     2401 cctatggctt ccatatctac tgaccttatt gtgggcttcc ctggtgaaac acaacaagat
     2461 tttgaagata cgctcaatgc tgtacgcacc atacagtttg atgaagcatt cacatacaag
     2521 tattcgccac gtgaaggcac gaaagctttt acactccccg attcagtacc agacacaata
     2581 aagcaggaaa ggcttgatat cttgattcaa actgtgcgtg agatttcaca aagaaggttg
     2641 atgaatcaca tacacaccac aaccgatata attgtagaac gtatcagcaa aaagtcggaa
     2701 aacgaagtta tggggaaaac catttgtaat catcctgtta ttatcaaagg tgggaaagaa
     2761 gatattggaa aaaaatacac agtaactatc gtatcattaa aaggttcaac gctctatggt
     2821 gagcgtagtg cttaaaacga tttgtattgt agcattatgt accaccactg ccttagctgg
     2881 tgaacagtcc gattttgaag caattcaaaa agcattcaaa ttaaacagta gcaacaagca
     2941 ggaacttgta aaaaaatatt tcaagactta tcccaacagt aactatattc ctgatatcag
     3001 gatgctttta gctgaatctg cttcaacagt ggatggaagc attgcagaat accataaatt
     3061 actcaataac tatcgctatt atgcaaaacg tgatgtagct tttttatcgt tatgtcagct
     3121 ctattatata aaaagcgatt ggggcaacct gcttactata tgcaacaagg caattgcaga
     3181 gtgtagtacc agtaactatc gcccaatatt tttacaatta cgggcgatag ctcattatca
     3241 tcacaaccat tatgatgatg gaataaaaga ttgtgaaaag atacctgaat actcacgcca
     3301 gctaaataca ctggcgtaca gtttgttctt aaaatcagag atggaccgac agatgtacgg
     3361 atatgcacgt caatacatta aaggtcttgc cgaactgtta catggctata cagaagcagc
     3421 tctatatccc acctgtttac tgcaactggg acaatactac gaatataata aaatgtataa
     3481 tgaagcatgg tcaacgtata attttttgat gagccaattc cccaaatcac cagaagcagt
     3541 gaaggcaaat acgtatgctc aaaatgtcag gaagtataat cctaaaactg ttgcatttat
     3601 gcctgatgaa aaaattattg cacagagtga ggctattgat attaaacctg atgtagaaga
     3661 gagtgacaca cagcaaaatc taaaacttta ctataccatt tctattggac ctttttataa
     3721 tttacaaaaa gcaaaagaat tgcaaaaatt aattgtcaat tatgtggtgc cagtacatat
     3781 cgtaagaaaa gaaaaatctt ttgatattta tgcaggccat ttcccttcac ttgatgaagg
     3841 tatgagtata aaaatacagt tggctgagga gttaggcatt aacgggttta taattgaaat
     3901 ttttgaacag gatgggaagc agtatatcta tgggcaatga taaagaacta actccaatga
     3961 tgcgacagta tcttgaaatc aagcaacatt acaaagatga gatactgttt ttcaggcttg
     4021 gcgattttta cgaaatgttt tttgatgatg caaagattgc ttcaaaagtt ttagatattg
     4081 cactgacttc taggcaaaat gacattccca tgtgtggagt gccataccat gctgctgaca
     4141 gctacatagc aaggcttatc aaagcaggat accgtgtggc aatatgtgaa cagatggaac
     4201 caccatcttc aagcactatt gttaagcggg aagttatccg cgtcattaca ccaggtacgg
     4261 ttgttgaaag caatcttatt gcatcagatg aaaacaatta cctgggttcg gtggttattc
     4321 ataacaatac ggtttgtact ggatttgttg atgtatctac aggcgatttt tttctttcat
     4381 ctgcagatta tagtcgtgaa ttcattgcaa ctgaaatttc ccgcttttca ccaaaagaga
     4441 tgttattgta cggttcacag caggaactta cgtttttgca tgacctttta aaacagcagg
     4501 atattgctct ggcatctatt aatgaatggt attatgatgt tgaatatctt actaatataa
     4561 tttgtaatat ctatggatta agcaatatta agggattggg attaacacat acagcaattc
     4621 ttacagttgg ttcactgctt cactatttaa aagatacgca taaaagtgca ctggttcacc
     4681 ttaaatatcc aaaaaatgtt agccgcagtg aatttatggt tttagaccac caaactatac
     4741 acaatcttga aatattgaaa agccaggatg gctcaaaact tcactcactt tttggtgtgc
     4801 ttaatcacac acaaactgca atgggaaagc gaacattaga acgggctctt ttgtatccat
     4861 tgcttgatgc atctgcactt gaaagtcgtt atgatattat agcatatctt attgacaatg
     4921 tacaattgtt aaaggaaatt caggaaaaat tatcacgtat tcatgatctt gaacgtattg
     4981 tttctcgttt tgcattgaag aaatattaca acagggattt cattgctcta caacaatcca
     5041 ttgaagctgc cagtgatgta tatgcagtat tgcagcaaca tcctgacaac ctttttaata
     5101 tgctgggtac tatcagcgat tctttacagg aactatcgct caaaattaaa aatactatag
     5161 ttgataatcc accactttcc tcagaacatg ggcgtgttat aaaagaaggt gttaatgctg
     5221 aacttgatag actgcatacg ttaaaaactg atgcacggca gtggcttctc aattatcagg
     5281 aagaagaaaa aaggcgtctt gatattccta cattaaaagt acgctacaac aaagttattg
     5341 gctattacat tgaagtgagc aagggtcaga ctggaaaggt accacaggat tatttcagga
     5401 aacaaacgtt aattaatgct gagcgatata caactgatac actacagaag tttgaaacag
     5461 atgtgctgca ggcacacgat tcaattatca agattgaaac tgagatactt gagtcaattg
     5521 ttgaaacaat actagctaac aagcttcaga ttcaggaatt agcatatact attggcatac
     5581 tggatatgtt ttgttcattg gctcttgcag catatcaaaa taattatatt aggccaatgt
     5641 tgaacaatga cggtattatt gacattaagg ctggcagaca ccctgttgtt gaaaaatttt
     5701 atattaaaga accttttgtc cccaatgaca tttttttaga tacagttgaa aacgttattg
     5761 ctattatcac gggccctaat atgtcaggta aatcaacgta catacgaatg gctgctgtat
     5821 tgcagctaat ggctcagatt ggatcattta tacccgcaca attagctaat ctgtgtattg
     5881 ttgatagaat atttacacgt atcggtgcca gtgacaatat tgcacgcggt gaatcaacat
     5941 ttcttgttga aatgaacgaa acagctatta tcttaaataa tgctactgat aaaagcttga
     6001 tcattatgga tgagattgga cgtgggacaa gtacttacga tgggatgtca atagcctggg
     6061 cagttgttga atatattgcc aattacatca aggcaaaaac attatttgct actcactatc
     6121 atgaattaac taaaataaat aaaaaaggta ttgttaatta tacagtgatg gtgaaagaat
     6181 ctattagcgg ggttgaattt ttacataaag ttgttcctgg tgcggcggac aaatcgtatg
     6241 ggattcatgt ggcaaaactt gctggtattc caaaacccgt aactgtaaaa gcaaacaaaa
     6301 tcctggaaaa acttgaaaaa tcttcacgga ttgattcttt aattaaagct cctgataatg
     6361 aagaacaact tgaaatattc caggcaacgc atcacagact ggcacaggct cttaaagata
     6421 taaccgttga aaatttaaca ccgcttgaag cgctcaatga aattgcacgc ctcaagcggc
     6481 ttattgaatg attcatttct tacggcctgc taaaaatggt tcaaccagat caattggtat
     6541 tgggaacagt gtggtagaat ttttctcggt agcaacttca cgcaacgtct gtaaataacg
     6601 aagctgcaat gcaataggat gcggttccat aagttttgct gcttcaacca tttttgacgc
     6661 tgcctggtat tcaccttcag cgtttataac cttggctctt cgttctcgct ctgcttcagc
     6721 ctgctttgcc atggcacgct gcatttcctg tggcaggtca acatgcttga tttcaacagc
     6781 agtcacttta ataccccatg ggtcagtctg cttatctaag attttctgca attcatgatt
     6841 tattctttct ctgtcagata acaggccatc aagttcaacc atacctaaaa tacttcgtaa
     6901 tgttgtctgt gccagctgcg atgtagcata caggtaatct tcaacttcgg taattgcttt
     6961 attgggttca ataacacgga agtaaataac agcatttact tttaccgaaa cattatcgcg
     7021 ggtgataaca tcctgcggag gtacatccat ggcaactgta cgtaagctaa ccctgatcat
     7081 cttatcaaca aacgggatga ttataaccag tcctggacct ttgggtcctg tagggggttg
     7141 caataaacgg cctaatcgga atacaacagc cctttcatat tccctgatta tttttattgc
     7201 tgatacagct aataaaacta acagtataat caatgtaggt aatgcttgaa acatcatatt
     7261 cctccttaaa atttatttgt aataaaagac tttataaaat ttagaatgtg tattaaattt
     7321 cttatcaatt atttactgcg aaactttctt tacatacaac accatgcctt ccatttttat
     7381 tacttcaacc acatcacctt tttttacagg ttcatctgag atagcagtcc acagttctcc
     7441 atgaacttca accgttccct tcccagtaaa atctattgtg gcctcacctt tttcgccaat
     7501 cataccttca ataccagtaa cagccttttg tgtatgtact ttaattactg cacgaaccac
     7561 aacaaagata aaaagcaaaa gaacaataag aacagtaata attgaagtta acggaatttg
     7621 acctccaggc aaaggagaat caaacaggat cattgaacca ataataaagg agattatccc
     7681 acccaacgtt agtaacccgt aacttgtaaa ttttaattcc agtacaaaca gtattatagc
     7741 taaaataata aacgtaagtc cagcaaaatt tataggcaat actttaacag ccagtaaaaa
     7801 taaaaataat gcaatagcac caattacacc gggaactatc atgcctggat tctttaattc
     7861 aaaaccaata ccaacaacgg caattaaaaa caaaaagaat accatctgcg gatcagcaaa
     7921 ataatttaaa atcttttcct tccagtccat tgtatactga tgttgctgaa tgttttttgt
     7981 ttttaagaca acgtatatat cttttgtttt tatcttttta ccatcaattt tttctaagag
     8041 atcgttgata tcattagcaa caacatctat cacacctaat cggagtgctt cattattggt
     8101 gcttgaaatg gcttcacgta ctgccttttc cacccatgtt atatttcgtt ttcgtaattc
     8161 tgctaaactt ttagcataag caacagtatc atttaaaacc ttcttttcca taatcccatt
     8221 ctgtttctgg tccggactga aatttaaaaa tgggcttacc ggatgcatgg cacctatttc
     8281 agtacctggt gacattgctg cgatatgagc tgctatcata ataaatccac cggcggatgc
     8341 agcctgggcg cctttaggat aggtatatac aataacagga atttcagaat ccagtatagc
     8401 tttaataatg tcacgcattg aattaagcag tccaccaggc gtatccatga tcatcagaac
     8461 aaattgcata ttgtcctgat tggctttttt aattgaattg actatgtaat ctgcaatgac
     8521 aggatttact gaaccgttaa gttcaatcag ggcatattta tcggcaccaa atactgttga
     8581 agaaaaagca ataatgagca acattgatgt taataaacgg actttcatag aattccacca
     8641 gaaattgaat ttcatacaga acattttttt aatcgtgtta cctgcaactt tcttttatac
     8701 ctcaattata tacaatatat gttatttcaa acattagtca agatattaaa tataagaatt
     8761 ttaaaaatga ttgatatcat gcctaaattt tcatataaaa gcttttcttt ttatatgtaa
     8821 acataaaaag agttgcgtta tcaagccgat agttactata taataccatg aatatattta
     8881 cagagagaat atcatgagaa aaatatgttt tattgttatc atcatgctca catcactatc
     8941 tatcattcct ggacagcaat tggaaaagaa aaatatggat gttgtgggcg atagctctca
     9001 gaataaatca attgaagaca atacacaatc tttaactgca gaatcaccaa ctacacaatc
     9061 agccaaagat gaaaaaaaag atatcaacaa tacatcacag gaaaaaaaga aaataactga
     9121 agaaaagcaa aaggacattt cacaggataa aaaaattgaa gctgaaaaga gaaaaacaga
     9181 atggattaaa aaaaccattc aatatgggat tcaggatgac aggatagatg caataaaatc
     9241 aacattaacc ataaaggatc cttcacaaaa aaatgacatt attaaacttt tagatgaact
     9301 tatgcctcag gaaacaaatc ctgaagttaa acgcaccgct attacagttg ttagcgagct
     9361 taaaagcagt tcatcatcgt catggattat tgcttcttta aacgacaact cagaggaagt
     9421 aaaaattgct gctgtgtatg caattgatac attaaaagct acagaagcca tacctaaatt
     9481 agaaggtatt ctcaaggaac aaaaatttac agaaaattct aattttaccg aagcccttat
     9541 acggacatta ggtaaattaa aagatacaca gttaaaagat tttgctattg aacgcattaa
     9601 agatacaaaa actacagaca atttaagaca attatttata ttatttctgg gtgatctgga
     9661 agatatcagt tctaaagatt ttttacttgg aattgtcaaa gacaaagatg aagatacaac
     9721 aatacgttca tatgcagctg tagcactggc aaaaattggt gcaaaggata cagcaggcga
     9781 tcttcttgaa ataataaaag agatagatag ttatcccgtt aacaaaagaa aagattatta
     9841 ctctttttca atgtactgta ctgctgcact tgtaaaaatg ggtgatagta atgctattcc
     9901 acgtttgatg gaggcattac gcagtaataa tcccgttgta cgcttgcgag cagttgagtt
     9961 gatgaaagat ttaaaagaca aaagaactat tgacattttg aaatataaaa tacagtatga
    10021 tccaagtcca aaagtccaga aagcagctaa agaagcatta aaagaaatgg gtgaaaatgt
    10081 tgatgaatca aaataataca ataaaaaata tcattatgca atctttaacc ctatctatcg
    10141 caataatgat atgcctgtta ttaataatac tattttcaaa tgttattatt ttcccaatag
    10201 tactattatc cgaaagctat cccaaaatat tcaccaggat tgttgagatt gtcatctatc
    10261 ttggcattat tactacgatt tgctattttt taatttacag aatgatacat ttgcgtaaag
    10321 caggtatacc tgtaacattg agcatcagaa atgctgttat atcaccttta cgtttcttta
    10381 taattctttt gatgatattg ttttttatag ctgtagtgat tacatcgatt tatattattc
    10441 ttaaaacaaa ctatatgata ctttataaat taattcacca gtgatattcc aatggcttta
    10501 gttagcgatt ttgataaata tttcctgcat ctacccaaaa tatcccaacc agatgatttt
    10561 gaaaaattct ggcaaagtgc aattaatgaa ttaaaaaaaa cacctattga tgtccaatat
    10621 gtgcataaca aaagaaaatc cactggcaaa ttctctgcct atgatatgac atatagtgga
    10681 tatggcaaaa tccaactttt tgccacatta tatattcctg ataaaataac aagaccacat
    10741 gttgtcatat tatttcatga ttatgatgac cttgaaagtt atgctcagtt ccccattgat
    10801 ttaccttttg catactgttt tgttgaatta cgaggtcata gaaacatcat ccattatgat
    10861 caggaacaga acaaatatcc gggatttatg actgaaggca tacttgaaaa ggatgaatat
    10921 tatgttaaag ggctttactt agatggcctt cgaactattg atgcattacg tttacataat
    10981 gatcttgatt gcagtaaaat agcaattatt ggtaagggat tgggatcagc tgtagcttta
    11041 ttcacaacat caaactgtaa caggattgtg gcgctggttc ttgatacgcc atcattttgt
    11101 tatcttgata aagcccagaa tatagcaaaa agtgtgatag caaatgaaat aaattctttt
    11161 attgatgaac atcgaagtaa aaaatctaca ataaagaaaa accttaatta ttttgacgct
    11221 ttgcaattta tcaatgctgt aacatgtcca gtacttacaa tagttggctt taaagattct
    11281 ctgtcacctg cagaatgtgt ttttgcactc tttaatcatc tgcaatgcga taaaactatt
    11341 gaagtatatc ctgaggaagg tcacggtgca ggtggtattg agcagcaaaa acgaatgata
    11401 gaatggatta aaaatattat ggtgtaaaaa tttttaagcc tggttttaaa ccagggtacc
    11461 tatactattt attatttttt aaagccaacc attttaagta tattcattat ttcttcaata
    11521 cggaatataa aagcaaagaa aaagtacaat acaccatata acggaagcag tgtcactgca
    11581 agtattaaag gatgataatg aaggttgatc ttaataaaga atgctattgt agctgcaatg
    11641 agggcacttg tccataaata aaataaatat tttttttcca aaccaacagt accaatcttt
    11701 cttgatatac ttacctgtaa catcttatat tcaacccatc ctgacaaacc tgcagtaatc
    11761 gttaaaccaa cagttccata gaccatatca atgtggaaaa gtagaggtcc atactgtgaa
    11821 gcatagtaac ctgtgacact tgtcagtaat acccgtatta ttgcaaactt caatggtgta
    11881 cgggtgtcat tcatggcata atatgctgaa gaatataaac gactctgtgt tattgctaaa
    11941 agcccaattg ttgaacctgc cagcaccatc cacacaaaga atgtatcgtg tgtggtaaat
    12001 tttccagtct ggtagatagc tcctaccaaa atatcaccta aggcaaaaaa tgcaactgtt
    12061 gacggaacta tataaaatgc aatgcgctta attgaattgt taattctatt cctcagcttt
    12121 gatgcaatct cttcttgtgg accaataact ttagacatgg atggcaattc agcagcggat
    12181 actgacatgc caaatagact tattggcaac atgtataatg tttgtgcata ggataatgct
    12241 gcgacagctc cggttggcaa caggctggca agtaaggaat caacgtatgc actaacctgt
    12301 actacaccac ggctgaaaaa aaccggtata aaatttttaa ttacagttcg tacttctctt
    12361 gattgagtat caaaacgtaa tgtaacattt tttactattg ttaacacaaa aggcaattga
    12421 attaatacct gcatcaaact gccaacaact gatccggctg cggtataagt ggctaagtca
    12481 ttttgcgtgg aactatcgcc aaaaaataat aatgttatta tgattataag attccaaaat
    12541 accggtgcac tatacgataa aaagaacttg ccatgactgt ttaaaatgcc taaacaccag
    12601 gctgaaaata caagtattcc tgcgccagga aaaaatatac gaaccagtaa aatggtaagc
    12661 tctcgctttt caccctgaaa acctggtgca ataatatcga taaggagtgg tgttatcagg
    12721 attccaacaa acacaagtga tgaaattaca agtgacaata ttccagctat gctacgggca
    12781 agttttgtgg cctgatcaaa ctttttttct gcaagtaatt tagcatacac cggtataaat
    12841 gaggctgaaa ggacgccttc accaaaaaga ttttgcaaaa aatttggaat tctaaatgct
    12901 gccctaaacg catcagcagc atcagaatta ccaaagtaat gggcaaacac gcgatctcgt
    12961 ataagaccgg ctattctact taagaatata ccggtggcaa ccattactgt gttgaataaa
    13021 cttttgtctt ttgattctgt tttcatatta aaaatgacat atacgctctg ctattgttgg
    13081 catagaaata tataaattct ttaaatacta ttttagtata aagctaatgc attatctatc
    13141 cctgtctttt acggcagggg catataagca tttcttactt tataaagcta aggaaaaaat
    13201 attgattttt aaaacaacaa ttcacctgtc aaaatttgtc aatttgtaaa aataaaaaaa
    13261 taaattattt cttgtagtta ttatacacat agcagataat ttgatgttag catgttttaa
    13321 ggaggcaatt attatgaaga aaattataat atacactatt attatggtaa ctatcgcttc
    13381 catgtcagtt gcgggtgact ggccaatcta taagggtaat ttatttttta cgggaaataa
    13441 cgatgaaatt attgtttcaa ataaccagtt aaaatggcta ttcttagcca aagatagagt
    13501 atttaatcct gttgtaagtg atggaagaat ttattttgta gataaaaatg catttcttta
    13561 ttgtatcgat gaagattcag gaaatattat ctggcttaac gatgttcgat ccatatcttc
    13621 acagtttaaa gcatattcaa aatcagcagg gaaaattaaa tatccacttg tatatggtaa
    13681 tacagttatt gtcagcgatc ccattgctat ttatgctttt gataaacgaa ccggcaatgt
    13741 tatatgggca cgtactggat tacgtgttga tgaaacaaaa cctcaaggat taaccggaat
    13801 tacaacacaa atttctatag atggcatata tgctgatccc attattgatc aggatactat
    13861 ctattatggg acacggaaaa tatttgcatc acgagacatt aaaaacggcc atataatatg
    13921 ggaaaacaag gatattgcta cctatagtgg gtttcctgtt tactatgatc agtatatcat
    13981 aacgcaatca atgaattatg aaacaaaaac atttaccgtt ttttgcttag aacaaagaac
    14041 cggtaaagta gtatggcaaa gaaattttaa tcaaccagtt acgatattgc cacctgtagt
    14101 aaataagcat gtatatgtac caataaataa tgctgtatat tgtttagata taaaaaatgg
    14161 tgaaacagta tggcagaagt catataatgg caccatcacc tcgccattaa gttttacaaa
    14221 caggtctata ctgttttcgc tggacaatgc atcaattgtt gggataaatc ctgaaaacgg
    14281 cagcacaatt gcaaatatat ttgttggaca acaatcttct ccttactttg tcacaatccg
    14341 tgatgtaata tacattgtgt ataatcaaca taatgataaa aatcaaccag tagcaaaagt
    14401 taaagcaatg tatattaatt caggtaatat atggtgggaa ttcacagctc ccttccctgg
    14461 aggagtatcg cagccagtag catctgatgg aattttattt ttacctgctg gaaattatct
    14521 ttatgcaatc ggtgcagggt atatgggtag ccaaagttat gaaagttcaa aaccagaaga
    14581 aaccaaaaag ggtgaaccac aggaaccatc aaaattacct gatcagaaac tatctgaaaa
    14641 agaattggcc gataaaacaa taacaccaga atctaagcct gcaccacaac ctgcaaaaca
    14701 agaaatgcca acacgaaagt tgcccgttga gattacagat aataataaag caccactgaa
    14761 atcacgaata accattcaat acaaagatga aaatggagaa tacaaccaat caactgctgt
    14821 taacggtaaa ggtgatataa caattcctga caaagataac gttgaagtga tttttgaagc
    14881 agatggtcat gttccaaaaa aggtaattgt caataaaagc gatagtcaat tgaagatagt
    14941 ccttgatgaa atacaacctg gcaaagctgt agtggtggat aatattcact ttgattttga
    15001 taaatggtac atcagaaaag actctattaa tatactggat aaactggtag acatgatgaa
    15061 agctaatcca tacttaaaag ttgaggtatg gggacatact gactcaattg gcgatgatac
    15121 atataatcaa aaattatctg aaaaacgtgc tgatgctgta tgtgattata ttattaagca
    15181 tgggataagc cctgaaagaa tcagtgcaaa aggttttggg gaaacaaagc caatagcatc
    15241 aaacgataca aaagaaggaa gacaaaagaa caggagaact gaattctact ttaaatcctg
    15301 agagggataa agattttcta taatcagtag cggcgatatt agcccattaa attcattggc
    15361 ttttactgta aaaagtatat caacaggaat tttttgtgtt aagatatcgt tatatttatc
    15421 tgcagaattc catgccatag cttctaattt tatagaacca ttaataataa gcttacaatg
    15481 tttgccattt ataataaaat attggctgat ggtgcagttt tttgataaaa aaataaaagg
    15541 cttatgacct attccgtaag gttcaaataa ttttagttct tcaagtaatt caaaagtaat
    15601 gtcttctact aataattcat aatcatattc tgggatatta ttctctgtag cttcataaca
    15661 caatgtatga tctaattctt ttaacagtat gggaatattc tcttttttta ttgaaaaacc
    15721 aaaggcatgg ttatgccctc ctaatttttc aaaaagatgt tcatattttg atacacttgt
    15781 aaaaaaatca aaattatttc ttgaccgccc tgatccttta attatttctg catcaggttt
    15841 atctgaaatt acaagcacgg gttgtaacaa ctgatcagca agtctatttg caataatccc
    15901 cgttaaaccg tcaggtatct catcagaaca tattaccctt aatgcatgat tttcattaga
    15961 atcatcatta gaaaattttg aaactaacgt ttgaatgata gttttacgct ccttgttgag
    16021 ttgaataatc tcctgtaaaa gttctttgac ctttgatggt tttttctcta aaagaaaatc
    16081 ggcagccagc tttgttttgc caaatctgcc aggtgtattg agaagtggtg atatatccca
    16141 gccaatcgta tcggaattta tagtttttgc aatatattct cttattacct ttaatccaca
    16201 atgattggtt ttatcaaagt atgatatccc attactcaca ataattctgt tttcatctat
    16261 cagaggcatc atatcggcaa tagtaccaag tgccaccagt ggcagcatat cctggataaa
    16321 ttgtgatatt ttttttgatt gctgatattg tatcttgaaa agaagcattg tggcataatc
    16381 aacagggctt gggaataaat ccttaatatc accttttatt atttgatgaa ttggcgatag
    16441 ctcatctaca aagtctctga tgtcataatg catcccatgt tgtaataatg ctgcatctgg
    16501 tacatcgtaa ttaaaaagaa ttgcaccagt atattgtttt gagaggtgtt taatttcctg
    16561 tatgctgcta gctgaaccat gagctatcgc cctgcccctg ttaataactt tatataaaat
    16621 agaatcatgt gataatgcaa taactataat gtatttatca taatactttg tgtagcttaa
    16681 caataccgcc agacacaatt tataggtaac accaacacct gccagttcct tgaaaggata
    16741 ttgcgaagtc attaattttg ggttaattat tgccgcatct ggaagagttt cagatggttc
    16801 atgatgatcg catacaatga catccatacc caaacttctg gcatacgcaa tttcattaac
    16861 atcacgagtg ccactatcaa gggtaataat acaatcgatg ttattttttt taaactcttc
    16921 aataatagat attgttagcc catataattc atcgccaaca ggatagcggt aaaagtactt
    16981 tacattcaaa agatcaagaa ggtgagttaa taatgttaat gaagtgagac catcaatatc
    17041 tgaatcggca aaaatgccta tcttcttttt attacttaca gcatttttaa taatccttac
    17101 tgcttcatcc atctcattca taagaaatgg actgtagcac aatgtaaggt caggattgag
    17161 gaatgtgtag gcactataag ggctgtctat accccttaat aatagtaaat ctgcaacacg
    17221 atgtgaaatg ttaaaatgag ataataataa atcttttaca taggatgatg cactggtaca
    17281 cacaaatgcc acctttatcg tattttatct ttttttttca gcagccttct tggctaaaaa
    17341 gtcagctttt tcattaaaat tacgcataac atgctcaatt ttaatgttag gtatctgttt
    17401 ttttaattct aatactcttt cataaagctt tttaatatta ggattactta cctgatattc
    17461 tcctgacagc tggcgcacta ctagttctga atcacttctt atttttaatg ttctggtttt
    17521 aaagtacagt gccagttgaa ttgcccgtaa taatgctgta tattctgcct gattatttgt
    17581 ctttttacca atataagctg aaactttgcc tacaagcctg tcatcgccat catatataac
    17641 aatgccaata cctgcaggac ctggatttcc ttttgaagca ccatcaatat acattgtaag
    17701 aacggattct gtaggtattg ttgataaaac ttcaagccct tcaaatatat tgtctgatac
    17761 ttcagcttga tttttatttt ctgtaatact gcttttcttt tttactatta ttttactttt
    17821 tgcacgttgt ttatcatatt caaggataat tttacgtgct tcttcaatta ctgattgtat
    17881 ctcacttaca tcaacatctg ataattcagc aatcttttcc agactcttgc cttcggcaag
    17941 tagctgtaaa actctatcta tgctcaataa cattactttc tcctcagcct gggtagttta
    18001 acctacaact acaaaagatt tttttcacat gttatattaa tttttaaaat acaaaaaacg
    18061 gccacaatta gtacatgtaa ctgcctttga tttagcaact tcatctttga catacacagg
    18121 tacctggaaa tggcatccca tacaaatccc gttttcaagt ggaactatac cattgccttt
    18181 tgtttcaata acttttgcaa atctggccct taaatcaggt ggcaactctg tcatttgttt
    18241 attaaattgc gaggcattat cctctgcttc tttttgcagt aattcaagtt tattctgtaa
    18301 ttcttcaacg tcgttttcaa tctggtttaa tcgttttggc atttcctgct gtaacacact
    18361 cagttcgttt tctattgcat caacatcttc catatactgc agcatactat tttcaatttc
    18421 gtctttcttt tgattaagag tttccagttc tgtaatcatt gaatcaaatt cacgttgtga
    18481 ttttaaagaa ttcttcctgc tgtcaaggaa ttgaatacgt gcttccacat catgaagttc
    18541 aagttctttt tgtttaatgt tagtttttaa ctgattaacc cttgatcgta aatctgccat
    18601 tttggcttcc atagctttca tttcatttcg ccagaaatca atggcatgac gtgatcgggt
    18661 aatttcattt tttgctgcca gtaccttatc ccatatagat tgtaattcaa gcattgtata
    18721 tatttcaggg ctcatttcac ataccacact tcattggatt atgttcttta tttgatatca
    18781 tcaaaggtat atcgttacag tgtatttctt ttgtcaagta gttgtaaaca gtttggtata
    18841 aaaaatcaat gattggtttc tcggatacat aatgacctat atcaatgact gcaacaccat
    18901 tattattggc aaatacagca tcatgatatc caacatcacc agtaataata caatctactg
    18961 aaccatgtga aataacagca ttaatttttc gtgaacctgc accctgcaaa cacgccactt
    19021 ttttaatggg tttaccagaa tctccatagt atatcattga agtgctctgt aatagttgtg
    19081 atattttttt caatatatcg gataatgtta tatcatattc atagtcacct gtagcaccat
    19141 atcctatttc actaaaacgt ttatcaggga acaataatgc aatattctta agacccagtg
    19201 attttgcaag taaatcccag caaaccgcat ctgcattggt atgagcagcg tatacactta
    19261 tttcatgttg aatacatgct attaccatct ggcctgtact attaccatcc aatatatttt
    19321 ttattggctt aaaaaatatt ggatgatgcg ttatgataca attacaatta ttttcatgtg
    19381 cttcattaat aacaacagga gatacatcca tagcaacaag aatgcctttg ataggtttat
    19441 tatcaaatga aatttgcctt cctgaattgt cgtattcttc ttgatattcc agtggaaata
    19501 ctttttcaag ataatcaaaa agccctttta tactatgcat gtgtatgttc cgtcttgtta
    19561 atttttaata acgtttcagc tatttcagtt ggagtgttgc caataagata aagcatattg
    19621 tcctgcttta tttcaacaca tacaatcctg ttgttttcag aaatattggt tttctgcagg
    19681 ttgtcaatat ttaatgaagt gtacaccgga ataccaagtg cctgagcatt aattttatac
    19741 tcaatttcat tttttatatt tatagctgcg cgatagtgca tcggagtaat tttctgcata
    19801 aataatacca tcggatgatc cacaccaaat gcaggatcct taaccctgaa aattgaatca
    19861 ttataaataa taattccacc aggaaacata gctatatcac tggtatcagt aacatacggc
    19921 agagtatatg ctatgtttga agccccactt aaaaacattc tgccacattt agcagcatga
    19981 agtaatgaaa aagctttttg aatttcctct actacatgta tcttttcttc attatttact
    20041 atcgccacaa gatgattggt tggaccatga cctttgccca atggcagaga aaatgtgatg
    20101 gcctttgtga taaattcttt tgctttcgtt attgcttcta tggtattgta cccctttgca
    20161 acataggttg caattgctga tgaaaatgtg catccagtgc cgtgggtatt ttttgtctca
    20221 atacgcttat ttttcaaaac tgtgaaatct ttaccatcat aaataacatc gcttggatcg
    20281 ctttccagat gcccaccttt taccactaca taatgtgcac caaatgaata aatttttttt
    20341 gcagcatctt tcatatcatt aatattttta attgtcatac cagtaagcac ttcagcttca
    20401 tgaatattag gtgttacaac atgagcaaca ggaattaaat gttgaattac tgattgaatg
    20461 gcatcatctg ataataattt tgatcctcct tttgcaacca tgactggatc aacaacacag
    20521 ttattaattg aatattgttt aatttttgct gcaacaagct taacaatatc aggatttgcc
    20581 agcattccgg ttttagctga atgtatggga atatcagatg caactgcatc aaactgtttt
    20641 tcaatcatgt ccagtggtac aggcatatag tcaataaccc ccagtgtgtt ctgggcagtt
    20701 attgcggtaa tcacagtcat accaaaacaa cccagtgcag ttattgtttt taaatcagcc
    20761 tgaataccag caccaccacc ggaatctgat ccagctatag tgagtacatt gctaataaaa
    20821 gatgaggttt tcatttttat atccagttct tatatatcaa taaagggggt ttacatttat
    20881 cacaatataa ctattttttt tcaagaatta tttgttgtaa aaaattactt gtcatttctt
    20941 ctatctttat caaaatcaaa cattattttt gtatcaaaaa tacctttata taaaggtcgc
    21001 tccatgaaca ataccccatt ttcatgtaaa acaagcgata gatggactgt tattgcacag
    21061 gacacaaaat actggcgtgt tggcatatat caccctgaat tttctagcgc tgatcaaatt
    21121 actcaattag aaaaacacag ctgtcctgaa ttatttatat gtgccaatgc accctgtgga
    21181 atattattga tggttgacgg aaaagaaatt cagtatataa tgaaccccaa cgatgccata
    21241 atggtaactg aataccataa cggttttagg gttgatcctg aaggttactt tatagtagtg
    21301 gaacaaacag actttactac ggaatttata gaaagaaaaa caacatggta ataaatgcac
    21361 atacatctgt atattgtatt ctgggtaatc ctgtacggca ttcactttca cctgcaatgc
    21421 acaacgctgc atttacacat actggaataa atgcagtata tgttgcgttt gaacctgctt
    21481 caattaaaca ggcttttgat gcaataaaag cgctgggaat aaaaggtgca agtattacca
    21541 ttcctttcaa ggtagacgtt ttaccgctct gtgatagcgt tgatacttta gcatcacgta
    21601 ttggctcggt taataccctt gtcaacgctg atggaatgat acaaggatat aataccgatg
    21661 gctatggatt actcaggtca cttgagactc attctataga ttatcacaac aaaaaggttt
    21721 taatcattgg caatggtggt tctgcaagag ctatcgccta tacccttgcc cgatataacg
    21781 cacatataac tatatgtggc cgaaatagta acaatataac gcaacttgca aatgacttac
    21841 ggaaatactt taccaatatt gaaacaatac tgattgataa gcttacacct gacataaccg
    21901 cacataataa cattattata aataccacac ctgttggcat gaatcctgac atatccagat
    21961 caccaattga tatatcattg ctgcatcctc ataacgtagt ggtagatatt atttattcgc
    22021 ctttgcgtac tcagcttgtt cagaatgcac aggaaaaagg atgtactgta ataactggcg
    22081 actatatgtt attattccag gcatgcatgc aatttgaact ttggacaggc aaaactgcac
    22141 ccattgacat tatgcatcaa gcactaaaac aggcattgca ataactatgc tgccacacta
    22201 tttacaatca cttgccaata atgaatctca tgcaagtaat tttactaatt attctcctga
    22261 tacaattgtt gctgtattga atctgttggg caatcctcag cacaagctac catgcataca
    22321 catagctggg accaatggga aaggttcaac tgcacatatg attgctgcca tacttcaagc
    22381 tgcaggttac actgttgggt tgtacacatc acctcatctt atacgatata atgaacgtat
    22441 atcaataaat ggcaaagaaa taacagatga agaaatatct gaatatacag aaatgctgga
    22501 aacactatgt aacacacata gttatactcc aacgtttttt gatgcattta ctatgattgc
    22561 ttttcttcat ttctataatt cagtggatat tgctatcatt gaaacggggc ttggtggtag
    22621 acttgattca acaaacattg ttactccact tatcagtgtt ataactccta tttcatacga
    22681 ccatacagca atactgggat ctaccttaca tgaaattgca tatcaaaaag caggaataat
    22741 aaaaaaacat attcctgtta taagtgccac acaaaaacct gaagcaaaag aagttttaca
    22801 aaaagaagca tcgatgaagc aatcaccaat attttttgtt ggagatcata tacgctttac
    22861 tatcgtttca caaaaccctc ttgtctttaa tgttatggtt aatcattctg cttttgacca
    22921 aaataaaatt ttaaaaaata ttcactgttc tttaatggga gattttcagg cagcaaatgc
    22981 atcggttgct atcgccacag cattaatgtt aagaaacaat aattgggaga tagtacctga
    23041 acatatatac catgctatgt caaatctcta tattcctgga aggtgtgaag tcctgagtaa
    23101 aaaacctctt atgatatttg attgtgcaca taatcctgaa gcattagaac atactctaag
    23161 caatatatgc aaacaatatg cagattatga aaaaatattc tgcacaagtt tcatgaagga
    23221 taaagatgtt gcgaaaatgc tatctataat acagcgcttt acttcaagac cgatctttta
    23281 ttttcagata cctgatagtc gctgctatat ccctgatcaa gaaataaaag aaacattttc
    23341 aatgaatgtg tttactgaga caaaatcact tgctaaactt ttaaaaccac atatcgactc
    23401 taatagcatg gtaattttta caggaacatt caggatctat ccttatagca gaactatctg
    23461 ttcactattg cagtaatgtt tgaatactaa agatccctat atgcaacata taatggcaat
    23521 tttaagtttt taattaaaag tataattgca atgattaccg aaaattttta aaaatatctt
    23581 cttgatttta gttttattta taaatttatg gtagaataca tataataatt catggtatga
    23641 taactaaata agcaaagtaa aatattccca agaaaatgta tgatttaaga tatacaagaa
    23701 ataaatacta tttcacaagg agtttaaaaa atggatctat cagaactatt acctaaagaa
    23761 catgtaataa ctgttaaaag caatgaaaag caattggtta tcaaagagtt gattcagaag
    23821 ttacaggatt taggaaaatt ggataatgct gatagatact acacacaggt aatgcaccgt
    23881 gaaacactgg aaaatacagg tgttggtaat gggtttgcca tcccccatgt acgaactgat
    23941 tcagtcaaaa aactactaac catttttgga atatgcaatg aaccagtaga atatgaatcc
    24001 tttgacaatc agccagttaa atatgtcatg ctcagcattt tcccaacttc tttaagcacc
    24061 aaataccttt accttgttgg gatgatggca agaatatttg ccaacacaga aaaaagagaa
    24121 aaaattgatg ctgctcgaac tccatcaaaa atttatccaa tactcactaa agaagccaaa
    24181 ttatattttg actcaataac agaaataaat aaggaagaaa atcatattga aagtttgtct
    24241 ggtgttccat catctgatct tgatctttta atcagactag atcaattata tcgcttgctg
    24301 gatagtggtg ataaatcaga aactcttaca aaaaaaatca atgaattgcg gcgatttatt
    24361 gataaccgct cactagcata ttatgagaga atgcggcaaa aatgccagaa tccttttgca
    24421 atacttgaaa aaaatacctg tggtggatgc catatggtaa tcccgccagt agaaatggca
    24481 aaaattaaag ggaagaaatc cctagctgta tgcacgtatt gtggaagatt tttaattata
    24541 gtatgattcc ctataataaa tatctgaatc tatagcaaaa ccactctttt aggtattgta
    24601 taaaaggacg tcgtttataa ttttccaggt gtatttcttc actatttctt atatctttta
    24661 aaaattgttt tctcatcttt tcaccaaaag actggtcaag gataatagca ttgacctcta
    24721 aatttgctgt aaagcttctt ctatctaaat ttgatgaacc aactgttgcc catatatcat
    24781 ctataacggc tgtttttgca tgcaaaactg catgcttata ttcatatatt ctgacattat
    24841 tcttcaaaaa ccttttatat aaatatctgc tagcatgctg tacaaatggt atgtcgctta
    24901 ttgctggcaa tagtattcgt acatcaacac ctttttttgc tgcatgtatt aaagatttta
    24961 caatccgcat gtcgggaata aaatatgcgt tggtaatata aatatagttg cgtgcatttg
    25021 caatagctgc taaatatgtc aggtaaattg gtttaattct tctgcgtgaa tggctgctta
    25081 atgccattac gattgtatca ccaacaggtt ttataaccgg aaaatacttt gaaatatcaa
    25141 ccagggcacc tccatgcctg tgccagtttt caagaaaaaa gtattcaatg tcctttacaa
    25201 ctggtccttc tatttttatg tgtgtatcac gccaattctt ccccctgtat tttttgccag
    25261 catattcatt gccgatattc gtgccaccaa taaaagctat tttgctatca acaaccatca
    25321 attttctatg atcacgtaca taaagattaa aatattttcg ccatggtaca ataggatggt
    25381 attctattaa ttctacaccc atttttttca tatattcaaa aagtaaagct gaagtattaa
    25441 tgcagcctat agcatcataa ataacattaa cctcaatatc tttctttgcc ttagcagcta
    25501 attttttggc taatttccat ccaatgtcat cactgttaaa tatatatgtt tcaaggttaa
    25561 tactatgacg tgcactatct acagttttaa acaactcatc aaaaaacaga tcaccatcag
    25621 ttataagagt aactttatta ccaccagaaa atgctgaaat gccaaatttt tttattgttc
    25681 gcaggaattg tggcagaaaa aacctgtttt ttatcttttt aattatataa tcataactta
    25741 ttcttgtatt aaagcctttg tgataggatt ttgatttaat gactaaattt tttctaacag
    25801 atagcattat agaatattta aatatatttt agttatatat ttacagaaag gaatagaatt
    25861 aattcagtac tttatgaatg atgtacaact tcaggttctg taattttgcc ggtattgcca
    25921 tttttctcgg gtttaaaata aattgtatca tattttattc ccgcatacac acggtgacaa
    25981 tctttaaatt ttccatttaa tatttcaaca gcaagcctgt cttctatttc gttttgtaca
    26041 gttcttctga gataccgtgc tccatacttt tcattaaatc cacgtttaac caggtattgc
    26101 ttgacttttg gagaaacaat gagttcaata ccgtgctcct caagatgttc atttacttca
    26161 tttaacatca aatcaagtat ttgtttaata tggctttcat ttaatttatg gaaatgtact
    26221 actgcatcaa tacgatttaa aaattcaggg ctaaataacc ttttaacttc ttcatctaca
    26281 ttctgaaatt gtgcagtttg aggatcaatt tcattaaagc ccattttacc gatcttttga
    26341 aattccctgt tgccaacatt tgaagtcatg ataattactg tatcaataaa gcttgttgtt
    26401 gaaccaaagt tgtcggttaa ttcaccttct tcaagtacct gcagtaatat attgtaaaca
    26461 tctgggtgtg ctttttcaat ctcatcaaaa agaatcaccg aatatggcct tcgttttact
    26521 ttttcggtta attgcccacc ttcctcatag cccacatatc caggaggtgc acctattagc
    26581 ctggaaacgg catgtttctc cataaactca gacatatcaa accgtattag cgcatcttca
    26641 tcatcaaaca gaaattctgc taaagccttt gcaagttcag tttttccaac acctgtcggg
    26701 cccaaaaaga tgaatgaacc aataggcctt ctggcacttc tgagtcctgt tctagatctt
    26761 cttatggctc tgcttataac acttattgca tcatcctgtc ctattatgcg tttatgcaat
    26821 tcctgttcca tgcgtaatag tttctgagat tcagattctt caagtttttc aactgggatt
    26881 ttggtccatt gtgcaacaac agttgctatc tgtttttcat caactataac tgcatagtca
    26941 ttgatgcgct gttgccaatc acttattttt tgtgataaca gtgctttctt ttcaatgatt
    27001 gtatctcgaa tagatgctgc ctgttcatat tcctgtgcct tcacaagctc atttttacgg
    27061 ttattcagat cttcaatttc acgttcaagc atttcaatat ctacggggcg ttcacaattt
    27121 tcaagccttg ctttggatcc tgcctcatca ataacatcta ttgccttatc aggtaaatat
    27181 ctatcatgga tatatctgtc agcgtataaa acggcctttt taattgcctc atctgtatat
    27241 cttactttgt gatgcatttc atagcgttca cgtaacccaa acagtatttc aatagcctca
    27301 tcaacagagg gctcatcaac cataactttc tggaagcgac gttccaatgc agcatctttt
    27361 tctatgtaca tacggtattc atttaaggtt gtggcaccta tgcactgcaa ttcacccctc
    27421 gctaatgctg gtttcaatat atttgcagca tctatagcac cttcagccgc ccctgcacct
    27481 atgatggtat gtaattcatc aataaacaaa ataacattat ctgatgcttt gatttctttc
    27541 atgatgcgtt tcagacgttc ttcaaactca cctctgtatt tggtcccagc aaccacagaa
    27601 gccagatcaa gcgccagaac ccttttatta aagagtggtt caggaacatc atggtgtaca
    27661 atacgctgtg caagcccttc aactattgca gtttttccaa cacctgcttc accaatcaat
    27721 acagggttat tttttgtttt acgggataga atccttataa ctctttcaat ttcggaattt
    27781 cttcctatga cagggtccaa cttatcttcg gctgccaaac gggtaagttc aatggcaaat
    27841 tcatctaagg ttggtgtttt ggttttatca ttggtttttt gctgagaatt accttcagga
    27901 gtaacgccca gaatcttttg tacttcgccc tttatgacat tatagtcaat accagcccgt
    27961 ataagggtgt caatccctga acatgtccca tcacgaaaaa ttgataataa taaatgttcg
    28021 gtgcccacgt aattattttt taatttacgc gcttcatctt ttgcaatttc aataattcgt
    28081 gtaaaacgtg cactgagtgg tagattgcct aaaataatag tggatccgga tctgcgtata
    28141 gcctgctcta tatttcttct tagtatctca aagttaaccc ccatattttt caatatgcgg
    28201 gctgcaaccg aatcatcgtc ttttaagagg gcaagcatta tatgctctgg ccctaatgca
    28261 tctgaattta atcgtcgtcc ctctgcctgt gctaaaactt ctaatacctt acgtgacctc
    28321 ttggtataat caaacatgtt tttttactcc ttatcttcga atttttttcg aagatatgct
    28381 gctctaaagg catccccttc ttcactgctg gcaaattgcc tgccagctat tttctgaaga
    28441 tgagatacct gtatatgtat cattaaatca ttaattacag aaagttcaat attttttatt
    28501 atcgacaaaa caacccctaa tcttattagt gataagtgct ctatggcttc tgcgtaactc
    28561 attgatcgtg aaaacgttac aataccatat gaacgccata ccatatcttc cagtacatct
    28621 ttatgatgct gataatattc gtctcgtgct tcattttcaa gatcaataat aaggctgata
    28681 actttgtcaa tttcctctat tatatctatc tcagattttc ccaaagtagc tttattactg
    28741 ataatataca tgctaccaac ggtttttgtt gcatcatgga caattccttt caattgtgcc
    28801 tggtattctc tgaccaattt tatcacttct cccatgtttt ttgctgcagt aagcattggt
    28861 aaatgcatca ttgctgaaac acgtaggcca gtaccaacat tggaaggaca ggtggttaaa
    28921 aagccaaaat catcagtaaa agcataggaa atatatttat ttaattcatc atctatctca
    28981 tcagctaatt taaatgccga tagcatctgc aaacctggtt tgattacctg aatcctgata
    29041 tgatcctctt catttataag gatagaatat tgatgaccgg ggtcaaaaac aacactacta
    29101 ttgttactca attccatttc atgagttatt atatcccgct cacgtaaaaa acgcctatca
    29161 tctgaagtgc aatcttttaa gcttacaaac tgagcatgtt caaaatattt tgatgttact
    29221 actgcctggt gaacaattga ttccagcgtt gatatatcag cttcatccat cttgtttcca
    29281 aagggtaatg acggtaaatt tcttgcaagc ctgactcgag ttgtcatgac aacatcagcc
    29341 gaaggcccca cctgggacca gaatggtgat tgtgaaagca gctgttcaaa cattaaagcc
    29401 tcttttaacc ttttagtctt tttatttcat ctctcagttt agctgcttct tcataatttt
    29461 cattctgaac tgctatttcc agcatctttt caagatcgtg caacgacact gtggcaacat
    29521 tctttgacct taccgtatca atcattcctt ttgattttct gtgttcaatg tatttaagcg
    29581 gcattttgcc aatatggtat gttgacccat gatatccagc taaaatggaa ctcagtgatt
    29641 ctttgaagaa gctataacaa tcggcacacc ctactttacc agtacgcttg taatcaatca
    29701 aagtccagcc acaggtggcg caggtattgc tgtcaaccat gtctgtcatt tcttttgcat
    29761 ccaaaaacga catcatatca gataccgtta acgaaaaacc cccaccatga gaacttaaac
    29821 ccatatcgcg tgcacaattg tcacacaggt gtacttcagt cttctcattt ctgatgatct
    29881 cagtcaaatg aacagttgct tcacgttgtt tacatcgttc acacaacata aatcctcttt
    29941 atggttacta tcgcacttta attatactat caaattatat aatactataa aatatgtgta
    30001 tatgcaaacc atttttaaat cattttagta aaaatttatg attttgctta aataacaaca
    30061 atatattaat ttttattaaa aattatcaaa atctattgac attatacaaa tatattatat
    30121 gtagtttgca atcggggcaa cccgatttat ttttttgtag gatgattgta tgaaagaatc
    30181 caaaaaatta cagaatttaa tccaactgga caaacaatat ctttttcaga attacggtac
    30241 acgccttccg gtttgtttta cacatggaaa gggttgcatt ctatttgatc aggatggcaa
    30301 acgatatata gatttttttg ctggtattgc agtcaattca ttaggataca accatcctgt
    30361 actgaataaa gctttacata gccaggttaa taaaattctc cattcttcaa actggtattt
    30421 caataaagaa cagattgaag cagccatgtt actatcaaaa acagcttttc ctggcaaaac
    30481 actttttacc aacagcggta ctgaagccag tgaagctgca atcaaattag cacgaaaatt
    30541 tggtttatca caatcacata aaaaatatca tattatatcg tttaccaatt catttcacgg
    30601 cagaacattt ggcgccatgt cggcaacagc acaggaaaag attcattcag gttttggacc
    30661 gatagttcca ggatttatct atgtgccatt taatgatacc gctgcattta aaaaaactgt
    30721 aaaaaaatac aaagggaaaa tatgtgccgt aataactgaa cttatacagg gcgaaggtgg
    30781 tatcaaaatt gccgacagtt catttataaa agagatagat actgttacaa aagaaaacaa
    30841 tatactcttt ataatagatg aggttcaaac cggtattgcc aggaccggta agatgtttgc
    30901 atttcagcat tacgatataa ctccagatgt cataacaatg gcaaaaggtc tgggtggtgg
    30961 tgtccctatt ggtgcaattc acacaaaaga ttttcttgtg gattattttc ctaaaggtac
    31021 acacggcacg acatttggtg gtaatcattt agctacggct gcagcaaagg cagtacttga
    31081 gacattaagc aaagaaactg cacttaataa tatacgcaaa acaggcgaat taattattaa
    31141 ggaactatcg ctcatgaaat caaaatatcc taaactgata gttgatgtga gaggcattgg
    31201 gttacatatt ggtgtagaat tgtcaattcc aggtcagcct ttagtgcttg aagctttaca
    31261 gaaagggctg gtaataaact gcaccgctga aaaagtaata cgaatcatgc caccgttaac
    31321 aatttcaaca aagcttgtta aagaagggct tttggttctt gatagtattt ttgcaggagc
    31381 aacagtatga taaagatacc cagggtaaaa tcaaaagatt tattatcagt aactgacttt
    31441 tcaaaagaag aaattatttc aatatttgaa ctgtcaaaaa agcttaaaga tgaactgaaa
    31501 cagggaaaaa tcaattcata ccttaaaggt aaaaccctgg gaatgatatt tgaaaaaaca
    31561 tccacccgta cacgagtatc atttgaagtg gccatgtttc aattaggtgg gtatgctctt
    31621 tttttaagca gagatgatat acaattaggc cgcggggaaa ccgttgcgga tactgcaaaa
    31681 gttttatctc gatatttaga tggtatcatg atacgatgtt atgcacatga aactgctata
    31741 gagttagctc aaaattccac aattcctgta atcaacggcc ttactgacat gtttcatcca
    31801 tgccaggcat tagctgactt ttttactata tatgagtcga atagaaattt taaaaatgtc
    31861 aagttagctt ttgttggtga tggcaacaat gttgcacaat cattaatgct aactgccgca
    31921 atgctgggtg ttgattttgc attggcctgc cccaaaggct attatccaaa aaaagaggta
    31981 gtggaacagg cattttctat tgcttcaaaa tcaggaagtg ttataacact taccagtgat
    32041 atagcattgg ctgttgaaaa tgccacacat ctttacactg atgtatgggt cagcatgggt
    32101 caggaaaaag aagcttcacg aagaaaaaag gattttgcta aatataaaat aacaaaaaaa
    32161 atactggata aatgtgcacc tgattgcaaa gtgatgcact gcctgccagc tcacaggggt
    32221 gaggaaataa ccgatgatgt tattgattca ccacaatcaa ttgtgctgga tcaggctgaa
    32281 aacagaatgc atgtacagaa agctattctc tgcacattga tgaaataatc attaacgtgt
    32341 ttaattatta aaaacaacaa ctctattgcg gccctggctt tttgcttcat aaagcgcttt
    32401 gtcagcatca cgtataatat cttcaattgt ttcagaattt tctcttattt ttgaaacacc
    32461 taaacttaat gaaatcttga attgattgtt ttttttatcg tggaatgtaa gatcattgat
    32521 acatgatctg atgcgttcag ctacccttgt cgcttgaatg atactggttt caggaaggca
    32581 tataataaat tcttccccac cataccgtgc aaatatatct aaagaacgta atgatgttga
    32641 aatgctttgt gcaacggttt ttaacacctc atctcccgtt gcatggccgt atgtat
//
LOCUS       DAORYL010000028        31427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1203_length_31427_cov_12.108632, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000028 DAORYL010000000
VERSION     DAORYL010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 31427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31427
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1203_length_31427_cov_12.108632"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="PLV81_06985"
     CDS             <1..996
                     /locus_tag="PLV81_06985"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HOM09881.1"
                     /translation="RMEIIEINSYTEPEKLNIGLQFLLPKQIKENGLSESNIKYSEDM
                     MLYTIRHYTREAGVRSLERVIAKVCRKVAREVVKHGSSHVREITVDILHEYLGPIKFK
                     YGEKEQKSEIGLANGLAWTEVGGDVLQVEASLIKGKGQLILTGQLGEVMQESAHAAFT
                     YVRANAQYFNIPEDVLRKHDIHLHVPEGAIPKDGPSAGITMGVVIASLLSQIPVRCDV
                     AMTGEITLRGKVLPIGGLKEKVLAAHRAQITHIIIPKDNEKDLEKIPEYVRKQLIFHP
                     VTSMEEVLFLSLEEPEKFFKDRQKTEKLNEQWKDTVRESIISDVSNELLTKHNYT"
     gene            complement(1066..1818)
                     /locus_tag="PLV81_06990"
     CDS             complement(1066..1818)
                     /locus_tag="PLV81_06990"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOM09882.1"
                     /translation="MKLYHDLAEYYFTIEKCHRNINDDIHLIKELAANIPNPVLLDLG
                     CGTGEHLNELYKCGFTCTGLDNSKNMLTHAKLRFPNAANFILSDITNFDFFENFDIVI
                     SLFGSFDYMIHDEDVDRVLWNTWRALKNNGFGLFEIWNAIPVREIKEKPLTTVSNVTY
                     KNLTIKRERGFTLIPDNNKTIAQVDYRYTISDGNSTRHVKDKHIMRAFFKEEIEEFLH
                     NNGFKIVTIYANSKKEPFIKTSNKLLIHFKKN"
     gene            complement(1829..3574)
                     /gene="ptsP"
                     /locus_tag="PLV81_06995"
     CDS             complement(1829..3574)
                     /gene="ptsP"
                     /locus_tag="PLV81_06995"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOM09883.1"
                     /translation="MSKSIEGVIASPGIVIGKAYIYRGDNIIIPKYTITDDQVEKEIQ
                     RYDESLAKTKEEIKAIQSQIATSLSNDMADIFKSHLMVLEDPYLDQKVRETISKEKRN
                     AEWVLNDITLQLIQSLKSIEDEYLRERIIDISDINKRLINNLQKTHDESLADLKEEVI
                     VVATDLTPSDTAMMNKKYVLGFITDRGGRTSHTAIMARALEIPAIVGTQVGTSLIKHG
                     DTIILDAIHGKIIVNPSKDEIGEYEKYREDLKQLESELAAIIELPSHTLDNETIYIYG
                     NIEIPDEMNIIRDHGAQGIGLFRSEFLFLDKSLPDEEKQFEEYKKVVEFFNPHPVTIR
                     TLDIGGDKIYAFTKQYKERNPFLGCRAIRFSLENEFLFRPQLRAILRASAFGNVKLMF
                     PMISSVEELLKAKSILYEEMENLQKLGIPFNQNLPVGIMIEVPSAAINVDIFCKYCDF
                     FSVGTNDLVQYTLAVDRISEKIAYLYNPLNLAVLRLLKLIADVANQNNISLSICGEMA
                     GEPKYTMLLLGLGYRQLSMSSAFMYQIKRIIRSVTVEECKDMVNNLMQLDNTYDIENA
                     MMEVLNKKFPNMFFK"
     gene            3760..4488
                     /locus_tag="PLV81_07000"
     CDS             3760..4488
                     /locus_tag="PLV81_07000"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOM09884.1"
                     /translation="MNWQITTVIFILIAVAIALVSLFNATFNDVKGESLQQLRELMVK
                     KQIEARGIRDPRVLQAMLQVERHKFVPELSIPQAYEDHPLPIGYGQTISQPYIVALMT
                     ELCELDGSEKVLEIGTGSGYQAAILSLLAKEVFTIEIVEPLGKQAALRLKSLGYKNVT
                     VKIGDGYKGWPEHAPFDVIMLTAAPPTVPDALLSQLKEDGGILVAPVGDYHQELVKIV
                     RNGNNYSQKVITYVRFVPMVPAHE"
     gene            complement(4493..5539)
                     /gene="argC"
                     /locus_tag="PLV81_07005"
     CDS             complement(4493..5539)
                     /gene="argC"
                     /locus_tag="PLV81_07005"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOM09885.1"
                     /translation="MKKALIVGATGFGGLGLIEILLRHKGFQIAQLVARKDVGKKISE
                     VFPHLEGFCDMPVQSPEEINYDDIDIAFFSTPDKAGMQMIHTFYKKNIPVIDFSGDFR
                     FATVDDYTIYAKNKGMNTEHASPDVLAHTVYGLPEKYAEKIKKAKIVGNPGCFAIAMI
                     LGLLPAVETGIIDSETIICDGKTGVSGAGKNPGEANFYPQRYEDVNTYREGHHQHIVE
                     VENIVNAHSKKKHKILFIPQIVPLNRGILVTIYADVQSTITSDELFEMYKSYYTNKPF
                     IHITHRSCHTTDVRGTNRCQIRPSIDSRTGKLFITSVIDNLIKGQSGNAVQNANLIMG
                     FDETEGLMVPAFYP"
     gene            6131..7144
                     /locus_tag="PLV81_07010"
     CDS             6131..7144
                     /locus_tag="PLV81_07010"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOM09886.1"
                     /translation="MKKYNVVILGATGAVGIEFRKILLQRNFPINNIRFLGKTTVGQT
                     IEFGDRKVTVEPVTDDAFKGFDIGLFSAGASISREVARKAAESGCIVIDNSSAWRMEK
                     DVPLVVPEVNPNDVDWHNGIIANPNCSTIQMVVALKPIHDVAKIKRIVVNTYQAVSGT
                     GLKAIEELQIQVKDIMNGKPISQRKVYPHQIAFNVLPHIDVFLENGYTKEEMKMVNET
                     KKIMGDSSIQVTATTVRVPVFYGHSESVNIETEKKITPQEVRELLRSAKGVTVVDGPS
                     KNEYPLAIHAAGKDDVFVGRIREDESIPNGINMWIVSDNIRKGAALNAIQIAELLIEK
                     KMV"
     gene            complement(7185..8426)
                     /locus_tag="PLV81_07015"
     CDS             complement(7185..8426)
                     /locus_tag="PLV81_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOM09887.1"
                     /translation="MKPKRINNKITIDEIIKLSNELNTIQDLDILLEKLLFEARSAVN
                     ADAGSIYIKDKDELVFSHVQNDTLTKRLSPGQKLIYTTFRVKINTSSIAGFVASTGNL
                     LNIPDVYNIPPDVTYKFNATYDVISHYKTTSMLTVPLQTVNGAIIGVLQLINAKDDKG
                     NVIPFTKDDEPIVMLFANNASLVLQRARMTRAILLRMISMAELRDPKETGAHVNRVAT
                     YAIELYEQWAKRHHINEKEIETNRDILRMAAMLHDVGKVAISDLILKKPARFTEEEYQ
                     IMKSHTYLGARLFKDTQSQFDEIASIVALTHHENWDGTGYPGYIDLDTGKPLEKDSKG
                     NPLARKGEEIPIYGRIVALADVYDALSSKRVYKDAWNENDVLNEIKLASGKKFDPELI
                     DIFFSCYDFLKSIRLRYPDSE"
     gene            8698..10428
                     /locus_tag="PLV81_07020"
     CDS             8698..10428
                     /locus_tag="PLV81_07020"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOM09888.1"
                     /translation="MKLTVFYFDPNVSSMFVEKTDETGNKFYTLSSPYNFVYRDEIVA
                     RVIEVDDPADIVYHLDSGYKYYKSEKFKPFKAGDGIIFDSISGCYRASDYGFVVYDQR
                     TSRIQLKLPLQISKDKLKASYIIFPTKFLKLPVYKDIEQQLLANKIVGIIDKNEIESQ
                     MNEIDPHNPKVHRILVARGREPVNGYDDYYIPLVQIEKKAGKLLDDGRIDFKEVGAII
                     EIKKGQEVLQKIPGIKPEDGFDIYGEKVEATIVQKDGYYPGSNIVPSESNPLIFVSQI
                     DGCLDIEGKTLSVLPVAIIKGDVNYESGNIDFDGSVQIMGSVLPGFTVNATGDIYVDK
                     NVDDARLTANGDIVVKLGIGGKGEAKIIAGGYVKAKFVLNSSIEALGKIEIEDSIINS
                     TVFSNDAVIVTGRHGKIIGGETTARHEIIVNVAGSQLQNITKLTAGKSLIVERELAEL
                     RKQMEKYKTNVEEILKKIKNSFGEGVFENPKEFLNILPPVKKKNCLLLLKELSDNNKQ
                     LKMLLDQRDEIEKKYKLEKEPSISITDIAYPGVIITIKRSKLQIDKAMQNTKFYEDNE
                     TKRVVFTSAV"
     gene            10590..10751
                     /gene="tatA"
                     /locus_tag="PLV81_07025"
     CDS             10590..10751
                     /gene="tatA"
                     /locus_tag="PLV81_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009852022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOM09889.1"
                     /translation="MPGLPELLIIFFIVLIIFGAGKIPKIAKDIGSGIKEFKKAMNSD
                     DDVKDEKKS"
     assembly_gap    10761..10860
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10894..11940
                     /gene="rlmN"
                     /locus_tag="PLV81_07030"
     CDS             10894..11940
                     /gene="rlmN"
                     /locus_tag="PLV81_07030"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOM09890.1"
                     /translation="MKEFIKNYSLKEVESLFEAIGEKSYRAKQLFNWLYERNVDNFFE
                     MTNFSKDLRYHLDEQYEISPLKLVDRKKSVVDGTEKFLFKTFDGHFIESVFIRNDGTD
                     EGRLTICVSSQAGCPMGCAFCETAKIGFKRNLQTAEILDQINQVRRLTGLKNNNVVFM
                     GMGEPFLNYDNVLKAADIMNYTFGFHISVRKITISTCGMYEGIERFIDEKRPYNLAIS
                     LNDTDMVKRAKNMPVEKKYPMEKIIRLLENKFPASRNRVTIEYIMRKDNISEEDAQRL
                     KTMFRYNRIKLNLIPLNKGKHNLEIPTQEEINKFIKRLEIMNIPISIRKSAGSDIDGA
                     CGQLSGKHYSSNSN"
     gene            11961..14216
                     /locus_tag="PLV81_07035"
     CDS             11961..14216
                     /locus_tag="PLV81_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOM09891.1"
                     /translation="MDVNLFDWMKKHKFFTFVGIPVIVLTVILLYTSIVYIEWIHDKQ
                     KTLDKLFKYKQLIDRTEELKEGYSYTYSEIDVSAKVVDIPTRIYDRNNEIIGEFFEQK
                     REIVPYSYFPKWLIKAVIASEDRDFYVHNGVSLKGILRAFAVNLIHLRVVQGGSTITQ
                     QLAKVLFTDMERNIKRKIYEIFCALEIEKHYDKQDILSMYLNYIYFGNGAYGVEAAAK
                     MFFGKSIIDCNETECAMIVATISNPLIYSPLNNLDVSLKKTRRILKSMVDAGFLKQQM
                     AEIQYKNFLKQWDVQFSKDNTAESSLIGSFIFSLYRINRAPFFNEYIRRILVNKFSEE
                     VVKKGGLSVYTTLDATKQDIALAALKKGVQLQREYHENKAKYIKNPQRALDEMEKAKN
                     IEGAFVSINPFTGEIIAYAGGYSFSYQNQMDHVQQIRRQPGSSFKPVVYVAAIQDKVI
                     TPSTVMDDIPMTFKGGYSPKNYDGIYSGKIIIREALKKSINVVAVQVLEKEGYSRIMS
                     IIQKALNLSSSELDSRFYKTLSLALGTYELSPLENGVLHSTIVNGGDFIIPYGIRYVK
                     DYNGNIVWNNEEEVLLQIQEKRKEYGKIIDPVACAIVVSMLKSVFEEGGTASWVFKNK
                     SVPVDVAGKTGTSSDYVDAWFIGYTPDSVSAVWIGNKTGAISLGKGRSGGVVAAPVWA
                     DYVFAIYKDSEYKSFIIPEQGITKQTICLDTGLVPRKEGLCPRVAINELFYAGTEPGE
                     YCTMHILPESH"
     gene            14225..15091
                     /locus_tag="PLV81_07040"
     CDS             14225..15091
                     /locus_tag="PLV81_07040"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOM09892.1"
                     /translation="MKEDKLFIIFSLVVLSCFLLSFQLPVTNGTIHSTFGEPRPDHFH
                     NGVDISSIDKKVYPVKEGNLVFAWDKALFPLEQYPGVGNYCILEHNKKEKSLYVHLEN
                     NIHLQPRYALTDSLAFMGNTGRSYGTHLHFAILNHFIVSVNPFTVMPSIDDKKPPVIE
                     GVYIKVGDSYTRLREGGNIRLTQHHPLAIQCFDAIQKNERCGVFSLKVILNNVTVTDV
                     KFNAIKLDNKNKLTIKGLPFENLYDSKGNYLVTNVSYNPGINNLTVIAEDFAGNQTSQ
                     SISFNVVLDFKK"
     gene            15131..15535
                     /locus_tag="PLV81_07045"
     CDS             15131..15535
                     /locus_tag="PLV81_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOM09893.1"
                     /translation="MILYYRRKKNIKQVDMAKALHVSPSYLCKIERGVQEPTEEFKKQ
                     CAKYLNVSIARLFPESIPDKKEIINEFVQSKNKLWSIRISKGIKQYELAKMLRCSPSY
                     LSKIEKGLQQPTDEFKKVCARVLKVKEIEIFP"
     assembly_gap    15859..15958
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15959..16378
                     /locus_tag="PLV81_07050"
     CDS             <15959..16378
                     /locus_tag="PLV81_07050"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HOM09894.1"
                     /translation="YPASKSLSELLEEFLTNKIQIAVVVDEYGGTAGIVTLKRIIAEL
                     FGRGYTLWEDIPKAEIKKEKDVVIVPGDMQIDEFNALFNVLIDSSESETIGGYIIERL
                     GYLPKRRASIMVDDYILTVRYVTKNRIQSIEVKKEEL"
     gene            16375..17646
                     /locus_tag="PLV81_07055"
     CDS             16375..17646
                     /locus_tag="PLV81_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOM09895.1"
                     /translation="MMEIYIGSQILIIIFTFLSAFFSATETAIVSSNIINVQALVNKG
                     ITRARSIMQIIENPDSALVAVLIGNNISNIGATAFITFFASKALLIHDSRLFIVTIAQ
                     TIFFLIFCELFPKIVSRSKPEKFLLHTFWLFQVISLMFRPLVTIILAFSTVIKNAIHE
                     DDSRPLPISSRDEIDTLFKAVEKEGIIESQHYIYFNEILSFRDVRANEVMTPLIDIIS
                     VEKKQSIKHCIDLIEKTRFSRIPVFEERVDNIIGYIYYQDILKNKSVKNIDQIIRKPY
                     FVPSSKKIYELIDEMEEQEISMVFVVNEYGAVEGLLTDEDIAEEIVGEIQTRDHPQDD
                     SIKEIAPWKFSVAGDLDIEYFQRRFGIKIEKRDFETLAGFLLYVLGHIPEVGEKIHFQ
                     HITFQIEEATDRSIERVIVSIPRRKASIGQA"
     gene            complement(17630..17974)
                     /locus_tag="PLV81_07060"
     CDS             complement(17630..17974)
                     /locus_tag="PLV81_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOM09896.1"
                     /translation="MGCLFCNIITGKTPSTKVFENDKVYAFKDINPQAPFHVLVVHKD
                     HIKNIDELNEKNSYIMSDIFLAINEIAKQEKLDKGYRVIINNGTAAGQVIWHLHVHIL
                     AGKDDLGPMLVQ"
     gene            18109..19239
                     /locus_tag="PLV81_07065"
     CDS             18109..19239
                     /locus_tag="PLV81_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09897.1"
                     /translation="MRTIKDIHYLVYPEEEKSNTIVDRFLSSAIDNRIAYHNEEELNS
                     IINELQINTIDSKDVIILKPFLGRFYQVCPGSPYMICCRYRVINTCFNCLYNCSYCYM
                     HVYLNNYGIVQFTNTERLFAELHDFIKNCLQSMVYRIGTGEFTDSLMFDEITGIGSEL
                     IQFFSKYNNIFFELKTKSANIDHLLDIPSKGNAVIAWSLNTPHNIAYYEHDTARLDER
                     IHAAVKAIDAGYYVAFHFDPIILYHGWENDYYNVIKLLQEKIDPEKVVWISMGGFRYH
                     LHFREILRDKFPEEMMTVYEMFPGIDGKYRYFKPLRIQIYNFMYQQLHEIFKKAYIYL
                     CMEADYMWQEITGKGFHSSEELEQDISNHLSYHFLQKNTIFY"
     gene            19317..20441
                     /gene="prfB"
                     /locus_tag="PLV81_07070"
     CDS             19317..20441
                     /gene="prfB"
                     /locus_tag="PLV81_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_144428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOM09898.1"
                     /translation="MLEYTDVKDLMRELTQIKASIEELYTALHIDDKKKQLQLLVEET
                     YKENFWQDQARSTLVQQQITQLKKNIEPWDILRKDIEDSIMLLEMAIDENDTDVIGDI
                     ASRVEQFTKRFEQLETLELLSDPDDDKNAIMTIHPGAGGTESQDWASMLMRMYTRWAE
                     DNGFDVEILDYMPDIEAGIKEVVMLIKGEYAYGLLKGERGIHRLVRISPFDSNQRRHT
                     SFASVDVIPEAPEDIDIEINESDLRIDTYRASGAGGQHVNRTDSAVRITHIPTGIVVQ
                     CQNERSQHKNKAYAMKILKSRLYELRKRELEKEKLEKFGEKKDIAWGNQIRSYVFHPY
                     TMVKDHRTGEETGSVDHVMDGDINRFIYAYLKSLKSIKIK"
     gene            20603..21799
                     /locus_tag="PLV81_07075"
     CDS             20603..21799
                     /locus_tag="PLV81_07075"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOM09899.1"
                     /translation="MIHTEKYTIFFLIILLLFIFTPQYTYAAGSGGTSGADFLEIGVG
                     SRPLGMGEAFTAEVGDVNAIYYNPAGLGTLKFPVMSLMHQELIVDSRFENVSLAFPVW
                     NGFFGFSTSVFWVPPFDKIDINGNKIGTVNFYNVSGIVAYGYSLGFMEVGGSIKYIYQ
                     QIDTLQLQSFAFDAGILKRITMYSPFDAPIRNLSLGLSIQNIGTNAKDDSLPRLIRAG
                     LSYKMSHFFGFNVDLIENAITSSDLYDFTSGFNESFRINAGVELNYLDILFFRGGYRF
                     NDAGKYTFGLGFNYAIKNIAFVIDASYQDAGVFGPIYSFTITFKLIPKVITIEDKLKA
                     EVHYQRGMKYFITDDIDGAIEEFKKARDYNPYHKNVDKKIQDLEELKELKRQNELLEK
                     EQQKIR"
     gene            21762..22487
                     /gene="rph"
                     /locus_tag="PLV81_07080"
     CDS             21762..22487
                     /gene="rph"
                     /locus_tag="PLV81_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013387557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOM09900.1"
                     /translation="MSYLRKNNRKYDEIRKVTVIPQFCPAALGSVLYTCGNTQVICAV
                     SQSPQVPQHAMDKGIGWLTAEYTLLPYATNPRTERRNLRPDGRSVEIQRLIGRSLRTV
                     LHLEAMQGVSLVVDCDVLQADGGTRTAAISGAYIALQLAVNSLLKQNIIAENPVKAQV
                     AAISIGYIGKELLLDLDYEEDSQCDVDMNLVMDSNNNIIEIQGTGEKRSYSIQELESM
                     LQLAQDGIQKIFKIQNDMLKLSL"
     gene            22550..23281
                     /locus_tag="PLV81_07085"
     CDS             22550..23281
                     /locus_tag="PLV81_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09901.1"
                     /translation="MRKIIITILFITFTHHVYAQYYIRNTTVIDTPTAYTIARGTYQL
                     SFLGYDNGGVELKAFIGLHNNFFLGASLDIQNAIGKDDPDPNVPGVVGKIKFTDGWEM
                     FPISFAIGYDSFYIGEEGKTYNSKNELNQMIYGPYFVITKPIYLFNDEQHIHFGMRIP
                     TQPEYVPEDTSYFLALDIPLGEIFIFKAEMERVYYNFDRADEWLYNVGMRYSYMQRLG
                     IEFDVLFQKDENPNRIIRIEYINEF"
     gene            23304..23804
                     /locus_tag="PLV81_07090"
     CDS             23304..23804
                     /locus_tag="PLV81_07090"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOM09902.1"
                     /translation="MKIFKKNSIFVLILLNAMYFVIVQKYYYNGKLFVYAQNKKNCVI
                     YIINTINRPINCNVEIADTGEEHAKGLMGRSELCDNCGMLFVFDDEDYRTFWMKNTGI
                     PLSIAFIDKNGIINDIQDMKPYQTFPTYSSKYPAKFALEVNQGWFKKNFITTGSKVIS
                     NGCISK"
     gene            23785..24927
                     /gene="hflX"
                     /locus_tag="PLV81_07095"
     CDS             23785..24927
                     /gene="hflX"
                     /locus_tag="PLV81_07095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOM09903.1"
                     /translation="MDASVNRIPISENEKTILIGIHLPNDNEEEVFHSLDELRQLVIT
                     AGAVVVSKLVVKRISLDPAYIIGSGKLEEIKKIVLEQSIKLVVFDLNIIRPVQIRNLE
                     NFLGCRVIGRTEVILDIFARRAKTVESKIQVELAQLNYILPRLKGLGGVLSRLGGGIG
                     TRGPGEKMLETDRRHIVKRIQTLKKKLSHISMHRNVQRQERLQNEIIGAVVGYTNAGK
                     STLVNMLAKDDLFVEDRLFATLDAYTRIVYLDECHKVLLTDTVGFINNLPSHLVESFK
                     STLEEIKYSDFIIHVVGLDIDIEKAIKVVNHELQLLDCSNKPVILYFNKLDIAKEETI
                     QYVHLRFPDAILGSSLTGQGIQLLKERIIEITETLDKHKQNISVYS"
     gene            25180..26319
                     /locus_tag="PLV81_07100"
     CDS             25180..26319
                     /locus_tag="PLV81_07100"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOM09904.1"
                     /translation="MYDFKRYSFPEERKKNSFIYGFFSLLFILVIVIFTLIADKPQNN
                     NKKIASSNLSSVENKLIVDGIKMEDDMPPVETEGEVIFDDNFDDDIDLSNKIVVDSSK
                     SKKKEKSYNIEELKKRDKRWHHTKYQIQKNDNIWKIAQKFDTNFKLIILANNINNPDI
                     LKPGRNIIVPNRKGVFHKVKKGQSLIAIARYYNVPLHAIIAHNSLEDPNCIIQEQTIF
                     IPDAKIDLRDKKDKRNIAKKEPVSVINRIKSQISLMWPVSGKITSGFGTRRNPLGKGR
                     QFHCGLDISCNVGTPVKAALDGTVIYAGWKDGYGNVVVLKHDKGYISVYGHNSKNLVK
                     EGDSVQKGQVIAKSGMTGSVTGAHVHFEFRKYITPLNPLRYLKEK"
     gene            26385..27395
                     /locus_tag="PLV81_07105"
     CDS             26385..27395
                     /locus_tag="PLV81_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM09905.1"
                     /translation="MSTIFATGQMKYDNPGQIIPVFYTQNDYVAWKAIVKGRIISIGE
                     REDTAKQELTRTVQPKTKVTVRLYNTDGIHKGTTLYVINTNNLIVSRFTVETIFRSQS
                     FGDMLVGYGYFRLSTIGDRVIQRFEGAYPEFAKIHKARGDYYKNTGEFGNAIKEYNQA
                     IILDASYPEAHIELGKIYFNDNVLQFAFNEFEQAYKNKDRIVDKQDIYELLYYMAKTR
                     FIEAYETNIPASLKEKYIKDGIKYAKEALIIMPDSIEVLYMLGIMYYKQTDPDDVEAK
                     KIFEKVIELDPTHTGAYIALSELYMKHKNNNKANYYSKKALEIDPANERARFIYRMTK
                     EK"
     gene            27524..28309
                     /locus_tag="PLV81_07110"
     CDS             27524..28309
                     /locus_tag="PLV81_07110"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOM09906.1"
                     /translation="MVKFLQNEIVRSLLPLIIINALALLSFIVFLFIYPKRPKDPEIV
                     ARMHETFMGLIFREFWYWVNQPFINFFIYFKIKPNTITAISLILAFVSAYFYYIGNFG
                     LAGWILIVSATLDIIDGRVARTTNTVTKSGAYWDSCVDRYSEGVVFLGIALYYQNNFI
                     ALLATIVALIGSELVSYTKARGEAIGITTKRGVMQRAERLTILCVISVLHPFFQVLFK
                     NSSVNPEIVMIGAMILMAVTTNLTAATRMRIIFREIKKTESNV"
     gene            28302..29573
                     /gene="mtaB"
                     /locus_tag="PLV81_07115"
     CDS             28302..29573
                     /gene="mtaB"
                     /locus_tag="PLV81_07115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOM09907.1"
                     /translation="MYEKTFSIKTLGCKCNQYESAQIASQFLSSGFKAVPFGSPADIV
                     IINTCTVTDKSNKKCRNYIRQGSKFSKTGKVIVTGCMVETHKDELDAMNEVQASFTNM
                     QKDKLVILSGAIGTDNENTSISTIANAYALPFMRTRGYIKIQDGCDGGCSYCIVPKVR
                     GNPTSRPVDDIVKHAQFLIERNCPEIVLTGITIGKYQYNDDTLASLIKKIITLEGNFR
                     VRVTSVEPTHVSEELIDILKHDKVCKHIHLPLQSGSDPVLMHMNRPYSVDFYTKLIDR
                     VRTSMPEIAIGTDIIVGYPTESDEDFEATVTLLKQISFAYVHQFSYSPREGTLSSNYR
                     PLPYDIIHARAQRLRDIARECSYQYRLQFLNTIRPAVIEKDGDGITALTDNYLKVALE
                     NNHYSRSKLGSIVPVMITSVSEEKIEGSIEL"
     gene            complement(29596..30540)
                     /locus_tag="PLV81_07120"
     CDS             complement(29596..30540)
                     /locus_tag="PLV81_07120"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HOM09908.1"
                     /translation="MQKTNSHFTSITSHSLIAAIGNTPLIELSKLSPKGSRILAKFEG
                     ANPGGSIKDRTAWYMIKKAIESGTLTPNKTILEPTSGNTGIGLAMVGSALGYKVVLCM
                     PKCVSVERQQTLIAFGAQLVLTEGCMGTDGAIIQAHKMYEENPDVYFMPNQFSNPANV
                     LAHYETTGAEILEQTGGQISAFVAGLGTTGTIMGVAKRLKEYNPKIKIIGVEPVPGHS
                     IQGLKNMQEAIVPDIYNPGMLDDIIIIDDETAFEHTRKLALQEGLFCGMSSGAAVAGS
                     IQVANDLKQGTVVTILPDRGDRYLSTSLFQSICAKCPP"
     gene            complement(30684..30755)
                     /locus_tag="PLV81_07125"
     tRNA            complement(30684..30755)
                     /locus_tag="PLV81_07125"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30721..30723),aa:Gly,seq:ccc)
BASE COUNT        10641 a         4713 c         5864 g        10009 t
ORIGIN      
        1 agaatggaaa taattgaaat taatagttac accgaaccgg aaaaattaaa tattggattg
       61 cagttcttac tacccaagca aattaaagaa aatggactga gtgagtcaaa tatcaaatat
      121 agcgaagata tgatgctgta taccatacgt cattatacac gtgaagctgg tgtaagaagt
      181 ttagaacgag tgatagcaaa agtttgccgt aaagttgcac gtgaagttgt aaaacatggc
      241 tcttcgcatg ttcgtgaaat aactgttgat atcctgcacg agtatttagg acctatcaag
      301 tttaaatatg gtgaaaaaga acaaaaaagt gagataggcc ttgccaatgg acttgcctgg
      361 actgaagtag gaggagatgt gttacaggtt gaagcttctt tgataaaagg gaaaggtcaa
      421 cttatactga caggccagtt gggtgaggtg atgcaagaat ctgctcatgc agcttttacc
      481 tatgtacgtg ctaatgctca gtattttaat atacccgaag atgtattgcg taagcatgat
      541 atacacctgc atgttcctga aggtgcaatc cctaaagatg gaccttctgc gggcattact
      601 atgggtgtgg ttattgcttc attattaagc cagatcccgg ttcgatgtga tgttgccatg
      661 acaggtgaaa taaccttacg tggcaaagta ctcccaatag gtggattaaa agaaaaggta
      721 ctggctgcac atcgtgcaca aatcactcat attatcatac ctaaggataa tgaaaaagat
      781 ctggaaaaga tacctgaata tgtacgaaaa cagctaatat tccatcctgt tacaagcatg
      841 gaagaagtac tgttcttatc attggaagaa cctgaaaaat tttttaaaga caggcaaaag
      901 accgagaaac ttaatgagca gtggaaagat acagtacggg aatcaataat ttcagacgta
      961 agcaatgaat tattgaccaa acacaattat acatagatta tgtaataaat attttgtaca
     1021 aagtaattat tatccatggc tatcaaacgg tcatggatat tttttttaat tctttttaaa
     1081 atgaattaaa agtttgttgg atgtttttat aaatggttct tttttggaat tggcatagat
     1141 agtaacaatt ttaaaaccat tattatgtaa aaattcttct atctcttctt taaaaaaagc
     1201 acgcataata tgtttatctt ttacatgtcg ggttgaattg ccatctgaaa tggtatatct
     1261 atagtctacc tgggcaattg ttttattatt atcaggtatg agagtaaagc cacgttcccg
     1321 tttaatcgtc aggtttttat atgtaacgtt tgatactgtt gttaatggtt tttccttaat
     1381 ttccctgaca ggtattgcat tccatatttc aaaaagtcca aaaccattat ttttcaaagc
     1441 tctccatgta ttccataaca ctctgtcaac gtcctcatca tgtatcatat aatcaaatga
     1501 accaaataaa gaaatgacta tatcaaaatt ttcaaaaaag tcaaagtttg tgatatctga
     1561 aagaataaag ttagcagcat tgggaaatcg cagtttagca tgtgttagca tatttttact
     1621 gttatctaaa ccggtacatg taaacccaca tttatataat tcattgaggt gttcgcctgt
     1681 tccacaacct aaatcaagta agacaggatt tggtatgttt gcagcaagtt ctttaataag
     1741 atgtatatcg tcattaatat tcctgtgaca cttctctatt gtgaaataat attctgctaa
     1801 gtcgtgatat aatttcatgg tgagtgaatt atttgaaaaa catattggga aattttttgt
     1861 ttaatacttc catcattgca ttttctatgt catacgtatt atccagttgc atcaaattgt
     1921 taaccatgtc tttacactcc tcaacggtaa cagaacgtat gatacgttta atctggtaca
     1981 tgaatgcaga actcatagat aattgtcggt atcctaaacc aagtaaaagc atggtatatt
     2041 ttggttcacc tgccatctca ccgcatattg aaagagagat attattctga tttgcaacat
     2101 cagcaattaa ttttaaaagc cttagtacag ccagatttag cggattataa agatatgcta
     2161 ttttttcact gatgcggtca acagctaatg tgtattgaac caggtcattg gttccaacag
     2221 aaaagaaatc acaatattta cagaatatat caacatttat tgcggctgaa ggtacctcaa
     2281 tcattatgcc aacaggtaaa ttttgattaa aaggtatccc caatttctgt aaattttcca
     2341 tttcttcata taaaattgat tttgctttga gaagctcttc aacagaagaa atcatgggaa
     2401 acatcaattt aacattacca aacgctgaag cacgcagtat tgccctgagt tgtggcctga
     2461 ataaaaattc attttcaagg ctaaatcgta tggctctaca tcctaagaag gggtttcgct
     2521 ctttgtattg cttggtaaaa gcataaattt tatcgccacc tatatcaagc gtacgaatag
     2581 taacagggtg aggattaaag aactcaacca cctttttata ttcttcaaac tgtttttctt
     2641 catcaggtaa agatttatcc agaaacaaaa attctgatct aaaaagccca ataccctgtg
     2701 caccgtgatc ccgtataata ttcatttcat cggggatttc tatattgcca taaatatata
     2761 tagtttcatt atcaagagta tgggaaggaa gttcaattat agcggctaat tcactttcaa
     2821 gctgttttag atcttcgcgg tatttttcat attcaccaat ttcatctttg gatgggttaa
     2881 caattatttt cccgtgtata gcatccagta tgatagtatc accgtgcttt ataagtgaag
     2941 tgccaacctg ggtaccaact attgcgggga tttccagtgc acgtgccata attgctgtat
     3001 gggaagttct gccaccacga tcggtaatga agcccaaaac atatttttta ttcatcatag
     3061 ccgtatcaga tggtgtcagg tcagttgcta ctacaataac ctcttctttt aaatcggcca
     3121 gtgattcatc gtgtgttttt tgtaaattat taataagccg cttgttaata tccgagatat
     3181 cgataattcg ttcacgcaaa tattcatctt caattgattt taagctctga atcagttgta
     3241 atgtgatatc attcaaaacc cattctgcat tacgtttttc cttgctgatg gtttcacgaa
     3301 ctttttgatc cagatatgga tcttcaagaa ccatcaaatg gcttttgaat atatcggcca
     3361 tatcattgga taaactggtt gctatctgcg attgtatagc tttgatttct tctttggttt
     3421 ttgcaaggga ttcatcatac ctttgtattt ctttttcaac ctggtcatct gttatggtgt
     3481 atttgggaat aatgatattg tcacctcgat aaatataggc tttccctatg actatacctg
     3541 gtgaagcaat aactccttct attgatttgg acataacaat gtacctgttg ccagtaacta
     3601 tcgcttacta tagtagtaaa tatgataaaa tgtaaatcaa aatatttaat tcaagtttga
     3661 aaaggaaatc aataattatt ataaaaaact tgtcaagata aataaaaaaa tttatattta
     3721 ttatcaaaag cattagcaca aaacagtata ttttaaaaaa tgaattggca aataacgaca
     3781 gttattttta tactgattgc agtagcgata gctcttgtat cattattcaa tgccaccttc
     3841 aatgatgtaa agggggaatc attgcagcag cttcgtgaat taatggtaaa aaaacagata
     3901 gaagcccgtg gaatcagaga ccctcgtgta ttacaggcaa tgctgcaggt agaacgacac
     3961 aaatttgtgc cagaactcag tatacctcag gcgtatgaag atcatccgtt gccaattggt
     4021 tatggtcaga ccatatctca gccatatatt gttgcactga tgactgaatt atgtgaactt
     4081 gatggcagtg agaaagtact tgagattggc acaggatcag ggtatcaggc ggcaatttta
     4141 tcgttgcttg caaaggaagt ctttactatt gaaatagttg aacctcttgg taaacaggct
     4201 gcgttgcgat tgaaatcctt aggctataaa aacgttaccg ttaaaattgg tgatggctat
     4261 aagggttggc ctgaacatgc tccgtttgat gtcatcatgc tcaccgctgc ccctcctaca
     4321 gtaccagatg cattgctgag tcagcttaaa gaagatggtg gcattctggt tgcaccggtt
     4381 ggtgattatc atcaagaact ggtcaaaatt gtccgtaacg gaaacaatta ttcacagaaa
     4441 gtaataacgt acgtacggtt tgtaccaatg gttcccgctc atgaataatt atttatggat
     4501 aaaaagcagg gaccataagg ccttcggttt catcaaatcc catgattaaa ttagcatttt
     4561 gaacagcatt gccagattga ccttttataa ggttatcaat aactgaggtt ataaaaagct
     4621 tgcctgtcct tgagtctatg gaagggcgta tctggcatcg attagttcca cgaacatcag
     4681 tggtgtggca ggaacggtgt gtgatatgga taaacggctt attggtgtag tagcttttgt
     4741 acatttcaaa tagctcatca ctggttatgg ttgattgtac atcagcatat attgtaacaa
     4801 gaatgcctcg gtttagaggg acgatttgcg gaataaataa aattttgtgt ttctttttac
     4861 tatgggcatt gactatattt tcaacttcca cgatatgctg atgatgccct tcacgatagg
     4921 tattgacatc ttcataccgc tgaggataaa aattagcttc acctggattt ttgcctgcac
     4981 cggatacacc tgttttgcca tcacagatga ttgtttcaga atcaatgatg cctgtctcaa
     5041 ctgcaggtaa taatcccaaa atcatagcta tggcaaaaca gcctgggttg cccacaattt
     5101 ttgctttttt aattttttct gcatattttt cgggcaatcc atacacagta tgtgcaagta
     5161 cgtcagggga agcgtgttcg gtattcatgc ctttattttt ggcatatata gtgtagtcat
     5221 caacagtggc aaacctgaaa tcaccgctga agtcaataac cgggatgttt tttttataaa
     5281 atgtatgaat catctgcatg cctgctttat caggagttga gaaaaatgca atatctatat
     5341 cgtcgtaatt gatttcttcg ggtgattgta ctggcatatc gcaaaaacct tcaagatgcg
     5401 ggaacacttc tgatattttt ttgccaacat ccttgcgggc aactaattgt gcaatctgaa
     5461 aacctttatg acgcaaaaga atttcaatca accctaagcc gccaaatccg gtagcaccaa
     5521 caattaatgc ttttttcata aatgttcctc cagggataga attttatcca aagtaagtaa
     5581 ttgctgtcca atcattgcta aaagcgcttt tttagcagtc aatagaaaat tatcacatct
     5641 ataatatcac tttatgatgg gcatagcaac aacgcaattc cttccatacc aggtcattgc
     5701 gagtgcaacg aagcaatccc agatttatca aagagattgc cacgtccctt cgagactcgc
     5761 aatgacgtga tcctatcgtt attgcgagtc ttgattgtat tgggatgacg aagcaatctt
     5821 gtttttaaga tttagattgc ttcaccactc cgggatttgc aatgacaaaa tcttgatgta
     5881 ttgtttattt aacctgaaat ttagttaaaa gtgactatcg cacaaaatga taaacattat
     5941 taatacgtta tgagtaaagc gaaaatattt gtcaattgcg aaatacaatt atggtaatga
     6001 ttttgaaata attaatgtga aaaataattt tttatgacta attgataaaa taaaaaagtt
     6061 attattgaca aaaaaaagtg ctatatatga caaatacaca ataagtaaaa atgcaggggg
     6121 atcattcatt atgaaaaaat acaatgtggt aattttaggt gcaacaggtg cagttggcat
     6181 tgaattcaga aaaattttat tgcaacgcaa ttttcctatt aacaatattc gcttcctggg
     6241 aaaaactact gttggacaaa caatagagtt tggtgatcga aaggttactg ttgaaccggt
     6301 aacagatgat gcttttaaag gattcgatat tgggcttttt tctgcaggtg cttccataag
     6361 tagagaagta gctcgaaaag ctgctgaatc cggctgtatt gttattgaca atagttcagc
     6421 ctggcgaatg gaaaaggatg tccctctggt ggttccggaa gttaacccaa atgatgttga
     6481 ctggcacaat ggcattatcg ctaatcccaa ttgttctaca attcagatgg tagttgctct
     6541 gaagcctata catgatgttg caaagataaa acgaatagtt gtaaatacct atcaggctgt
     6601 ttccggaact ggccttaaag caattgagga acttcagatt caggtaaaag atattatgaa
     6661 tggaaaaccc atatcacagc gaaaagtata tccacatcag atagcattta atgtgttacc
     6721 acacattgat gtattccttg aaaatggata cacaaaagaa gaaatgaaga tggttaatga
     6781 aactaaaaag attatgggcg atagctcaat acaggtaact gcaactacag taagagtccc
     6841 agtattttat ggccactcag aatcagtaaa tattgaaacc gagaagaaga taacaccgca
     6901 ggaagtacgt gaactgctac gttctgcaaa aggtgttaca gtggttgatg gtccctcaaa
     6961 gaatgaatat cctctggcaa ttcatgctgc cggtaaggat gatgtctttg tgggaagaat
     7021 acgggaagat gaatcaatac ccaatggcat aaacatgtgg atagtgtctg acaatatacg
     7081 aaaaggtgct gcgcttaatg ctattcagat tgcagaactt ttgattgaga aaaaaatggt
     7141 ttaaaaataa aggggcatgt aaaaagagca taccccttta tatattattc actgtcaggg
     7201 tatcgtaacc tgatcgattt aagaaagtca tagcaagaaa agaaaatatc tataagttca
     7261 ggatcaaatt tcttaccact ggctaattta atttcattga gaacatcatt ttcattccat
     7321 gcatctttat atactctttt agatgataat gcatcataca catcagcaag agcaacaata
     7381 cgaccataaa tagggatttc ttccccctta cgcgctaatg ggtttccctt gctgtctttt
     7441 tctaaaggtt tgccagtatc taaatcaata taaccgggat aaccggttcc atcccagttt
     7501 tcatgatgtg ttaatgctac aatacttgct atctcgtcaa attgtgattg tgtatcctta
     7561 aacaggcgtg cacctaaata tgtatggctt ttcataatct ggtactcttc ttcggtaaag
     7621 cgtgctggct tcttcaaaat caaatccgag atggcaactt ttcccacatc atggagcatg
     7681 gcagccattc gtaaaatgtc cctgttggtt tctatctctt tttcgttaat atgatggcgt
     7741 tttgcccact gttcatacag ttcaattgca tacgtagcaa ctctgttaac atgggcacca
     7801 gtttcttttg ggtctcgcaa ttcggccatg cttatcatcc ttagtaatat tgcgcgcgtc
     7861 atccgtgcac gctgtaaaac aagacttgca ttatttgcaa acagcattac tataggttcg
     7921 tcgtcttttg taaaaggtat tacattacct ttatcgtctt ttgcgttaat cagctgtaac
     7981 acaccaataa ttgccccatt aactgtttgt agtggaacag taagcatgga ggttgttttg
     8041 taatgtgaaa taacatcata tgtggcatta aatttatagg ttacatctgg gggtatatta
     8101 tacacatccg gtatatttaa taaatttccg gttgatgcaa caaaacccgc aattgaactg
     8161 gtgtttattt tgacgcggaa tgttgtataa atcagtttct gtccaggtga tagtcgttta
     8221 gtcagtgtat cgttctggac atggctgaaa acaagttcat ctttatcttt gatatatata
     8281 gaaccagcat ctgcattaac tgccgaacgt gcctcaaaaa gcaacttttc aaggagtatg
     8341 tctaaatcct gtatggtatt gagctcattt gaaagcttta ttatttcatc tatagtaatt
     8401 ttgttattta ttctttttgg tttcatgtat catgtaccct agaaatataa attattaaaa
     8461 tatacaataa ttacaataat ttgtccataa taatatgtga aagataataa tcaaatgata
     8521 cataatttca atgaattaaa atatttgtcc tttatattat tttaaatctg attattcaaa
     8581 attttagttg tatttaatta tctaatgatg tatactattt ttgtaaccga tagttactat
     8641 ataagggggc agaatgctga ttaaaagtat aatacaatca taccctggtg aagttccatg
     8701 aaattaactg ttttttattt tgatccaaat gtttcatcaa tgtttgttga aaaaacagat
     8761 gaaactggaa ataaatttta tacactttcc tcgccttata attttgtata tagagatgaa
     8821 atagttgcac gtgttattga agttgatgat cctgctgata tagtgtatca tttggattca
     8881 ggatataaat attataaatc tgaaaaattt aaaccattta aagccggtga tggtataatt
     8941 tttgattcaa taagcgggtg ctatcgtgca agtgattatg gatttgttgt ttatgatcag
     9001 agaacttccc ggatacagtt aaaacttcct ttgcagattt caaaagataa gttaaaagct
     9061 tcatatatta ttttcccaac aaaattttta aaattaccag tatataaaga tattgagcag
     9121 cagcttttag caaataaaat agtaggcatt attgataaaa atgagatcga atcccagatg
     9181 aatgaaattg atccacataa tccaaaagta catagaattt tagttgcacg tggcagagaa
     9241 cccgttaatg gatatgatga ttattatatt cctttagtac aaattgaaaa gaaagcaggc
     9301 aaacttttgg atgatggtag aatcgatttt aaagaagttg gtgcaatcat tgaaataaaa
     9361 aaaggacagg aagttttaca aaaaattcca ggtataaaac ctgaggatgg atttgatatt
     9421 tatggagaaa aagttgaagc tactattgtc cagaaagatg gatattatcc aggctccaat
     9481 attgtccctt ctgaatcaaa tccattaata tttgtttctc aaatagatgg ttgccttgat
     9541 attgaaggca aaacactgtc ggtattgcca gttgcaatca ttaaaggtga tgtaaattat
     9601 gagtcaggta atattgattt tgatgggtcg gttcagataa tgggttctgt gttaccgggt
     9661 tttactgtaa atgcaaccgg tgatatttat gtggataaga atgttgatga tgccaggcta
     9721 acagcaaatg gtgacatagt ggtaaaattg ggaattggtg gaaagggtga agcaaaaatt
     9781 attgctggcg ggtatgtaaa agcaaaattt gttctaaatt catctatcga agcattagga
     9841 aaaatagaaa ttgaagattc tattatcaac agtacagtat tttcaaatga tgcagtgatt
     9901 gttacgggaa gacatggcaa gattatcgga ggtgagacaa ctgcccgtca tgaaattata
     9961 gttaatgttg caggttcaca attgcaaaat attacaaaac tgactgcagg gaaaagtctt
    10021 attgtagagc gggaattggc tgaattgcgc aaacagatgg aaaaatacaa aaccaatgta
    10081 gaagaaattc ttaaaaaaat taaaaatagt tttggtgagg gagtgtttga aaatccaaag
    10141 gaattcctga atattttgcc accggtaaaa aagaaaaatt gtttgctact attaaaagaa
    10201 ttatcagata acaacaagca gcttaaaatg ttactggacc aaagggatga aattgaaaaa
    10261 aaatataaat tagaaaaaga accatccata agtataaccg atattgcata ccctggtgta
    10321 attattacta ttaagcgtag taaattacag atagataagg caatgcaaaa tacaaaattt
    10381 tatgaagata acgaaacaaa aagagttgtt tttacctcag cagtataagt ttgcaataat
    10441 taaaaataaa attagatttt tctgtataag atacatatat tataataaat attacaatat
    10501 ttgacactaa tatttaaaat atttgacaaa taattgtagt atactatctg tgagcaaatc
    10561 atattattat ggaggctttt attatgagta tgccgggact tccagaatta cttataatat
    10621 tctttatagt tttaatcatt tttggtgctg gaaaaatacc aaaaatcgca aaggatattg
    10681 gatcaggaat aaaagaattc aaaaaagcta tgaattctga tgatgatgtc aaggatgaaa
    10741 agaaatctta agaaattaaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 atataaagct aaacattcta tttagtttca tgtatgaaag aatttattaa aaattattcc
    10921 ctgaaagaag ttgaatcatt atttgaagct attggcgaaa aaagctacag agcaaaacag
    10981 ctgtttaact ggctttatga gcgcaatgtg gataattttt ttgaaatgac aaacttttcc
    11041 aaggatctgc gctaccatct ggatgaacag tatgaaatat caccgctaaa actggttgat
    11101 cgtaaaaaat cagtggttga tggtacagaa aaatttttat ttaaaacctt tgatggtcat
    11161 tttattgaat ctgtttttat tcgtaatgat ggcactgatg aaggacgatt gaccatctgc
    11221 gtttcaagcc aggcaggttg cccaatgggg tgcgcttttt gtgaaacagc taaaattggc
    11281 tttaagcgta atttacaaac tgcagaaatt ctggaccaga taaatcaggt aaggcgttta
    11341 acaggattaa aaaataataa tgttgtcttt atgggaatgg gagagccatt ccttaattat
    11401 gataatgtat taaaagctgc tgatattatg aattatactt ttggcttcca tatatcggta
    11461 agaaaaatta caatatcgac atgtggtatg tatgaaggta ttgaacgatt tatagatgaa
    11521 aaacgcccat ataatttagc aatatcatta aatgataccg atatggtaaa acgtgcaaag
    11581 aatatgccgg ttgaaaaaaa atatcctatg gaaaaaatca tacggttact ggaaaataaa
    11641 tttcctgcat cgcgaaatag agtaacaata gaatatatca tgcggaaaga taatatttct
    11701 gaagaagatg cacagcgttt aaaaaccatg ttcagatata accgtattaa acttaatctt
    11761 ataccgttaa acaagggcaa gcataactta gaaattccca cccaggaaga aataaataaa
    11821 tttataaaaa gacttgaaat tatgaatata cccatttcta taaggaaaag tgccggaagt
    11881 gatatagatg gtgcttgtgg ccagctttca gggaagcatt attcaagcaa tagtaactaa
    11941 aaaatcacaa ggcttatggt atggatgtta atctatttga ttggatgaaa aaacataaat
    12001 tttttacatt tgttgggatt cctgttattg ttcttactgt gattctgttg tatacatcaa
    12061 ttgtatatat cgaatggata catgacaaac aaaagaccct tgataagctt ttcaaatata
    12121 aacaactgat tgatcgcaca gaggaattga aggagggtta ttcatatacg tatagtgaga
    12181 tagatgtttc tgcaaaagtt gtagatattc ctaccagaat ttatgatcgc aataacgaaa
    12241 taataggcga attttttgaa caaaaaaggg agatagttcc ctattcatac tttccaaaat
    12301 ggcttatcaa agcagttatt gccagtgaag acagagattt ttatgttcat aatggtgtta
    12361 gtttaaaagg cattttacgg gcatttgctg tcaatctgat tcatctgaga gttgtacagg
    12421 ggggcagcac tatcacgcaa caactggcaa aagtgctgtt tactgatatg gagcgtaata
    12481 taaagcgcaa aatatatgaa atattttgtg cactggaaat agaaaaacat tatgacaagc
    12541 aggacatcct atcaatgtac cttaattata tatattttgg taatggtgcg tatggtgttg
    12601 aagctgctgc aaaaatgttt tttggtaagt caatcataga ttgtaacgaa actgaatgtg
    12661 caatgatcgt tgctactatc tcaaatccac ttatttattc accacttaat aaccttgatg
    12721 tttcactaaa gaaaacaaga aggattttga aatcgatggt tgatgcaggc tttttaaaac
    12781 aacagatggc cgaaattcag tataaaaatt ttttaaaaca atgggatgtt caattttcaa
    12841 aggataatac tgcggaatca tcgttaatag gaagcttcat atttagctta tatcgtatca
    12901 atagggcgcc atttttcaat gaatatatac gaagaatact ggttaataaa ttttctgaag
    12961 aggttgttaa aaagggtggt ttaagtgtat atactaccct ggatgctaca aaacaggata
    13021 ttgcattggc tgcgttaaaa aaaggcgtac aattacaaag agagtatcac gaaaataaag
    13081 caaaatatat aaaaaatcca cagcgtgcac ttgatgaaat ggaaaaagct aaaaatattg
    13141 aaggagcctt tgtttcaatt aatccattta ccggagaaat tatagcctat gctggtggct
    13201 atagcttttc ttatcaaaac cagatggacc atgttcagca aatacgaaga caacccggtt
    13261 catcgttcaa gccagtagtg tacgttgcgg ctattcagga taaggttata acgccttcaa
    13321 cagttatgga tgatatcccc atgacattta agggtggata ttcccctaaa aattatgatg
    13381 gaatatattc aggaaagata atcatacgag aagcattgaa gaaatctatc aatgttgttg
    13441 cagttcaggt ccttgaaaaa gagggatatt cccgtataat gtcaatcata caaaaagccc
    13501 ttaatttgtc cagcagtgaa ctggattctc gtttttataa aacattgtca ttggcgctgg
    13561 gtacatatga actatcgccc cttgaaaatg gtgtattgca ttcaacaatt gttaatggcg
    13621 gcgattttat tattccctat ggtatacggt atgttaaaga ttataatggg aatatagtct
    13681 ggaataatga agaagaggtt ttattgcaga tacaggaaaa gcgcaaagaa tatggtaaga
    13741 taattgaccc tgttgcatgt gcaattgtag tttcaatgct taaaagtgta tttgaagaag
    13801 gcggaactgc ttcatgggta tttaaaaata aatcggttcc agttgatgtt gcaggcaaaa
    13861 caggtaccag tagcgattac gttgatgcat ggtttattgg gtatactcct gattctgttt
    13921 ccgcagtgtg gattggcaat aaaacgggag caatctctct ggggaaggga agaagtggtg
    13981 gtgttgtagc agcacctgtt tgggctgatt atgtatttgc tatatacaaa gattcagaat
    14041 ataaatcatt tattattcca gaacagggaa ttacaaaaca aactatctgc ctggatacag
    14101 gtttggtacc gcgaaaagag gggttatgtc cacgtgttgc aattaacgaa ttattttatg
    14161 ctggaactga accaggtgag tattgtacca tgcatatatt accggaatct cattgaggat
    14221 atatatgaaa gaagataaat tatttataat tttttcctta gttgtgttat catgttttct
    14281 attatcattc cagttgcctg ttacaaatgg aactatacat tcaacatttg gggagccacg
    14341 tcctgatcat ttccataatg gagtggatat atcttcaatt gacaaaaagg tatatcccgt
    14401 aaaggaagga aatttagttt ttgcctggga caaagcatta ttccctctgg aacaatatcc
    14461 cggagttggc aactattgca tactggaaca taataaaaaa gagaagtcat tgtatgttca
    14521 tttagaaaat aatatacatt tgcaaccacg ttatgcgctt actgacagct tagcatttat
    14581 gggaaatact ggaagatcgt atggaacaca tcttcatttt gctattttga atcattttat
    14641 tgtatcagta aatcctttta cagtgatgcc atcaattgat gataaaaagc caccagttat
    14701 agaaggggta tatattaaag tgggcgatag ttacacaaga ctacgtgaag gcggtaatat
    14761 aaggttaaca caacatcatc ctcttgctat tcagtgtttt gatgccattc agaaaaatga
    14821 gcgttgcggt gttttttccc tgaaagtgat tttaaataat gttactgtta cagatgtcaa
    14881 atttaatgca ataaaacttg ataataaaaa taagttgaca attaaagggt taccatttga
    14941 aaatttatat gatagcaaag ggaattattt agtaacaaat gtttcgtata acccgggaat
    15001 taataatctg acagttattg cagaagactt cgcgggcaat caaacctctc aatcaattag
    15061 ctttaatgtg gttcttgact ttaaaaagta aaagcagttt ttcccaagga ggaataattt
    15121 tgtggctaat atgattcttt actatagaag aaagaaaaat attaaacagg ttgatatggc
    15181 aaaagcatta cacgtatcac catcatattt atgtaaaatt gaacgtggtg ttcaggaacc
    15241 tactgaagaa tttaagaaac agtgtgcaaa atatctgaat gtcagtatag ccaggttatt
    15301 tcccgaaagt attcctgata aaaaagaaat tataaatgaa tttgtgcaat ctaaaaataa
    15361 actttggagc attcgtatat ctaaagggat taagcaatat gaattggcaa aaatgttgcg
    15421 ctgttcccct tcatatcttt ctaaaataga aaaaggtttg caacaaccta ctgatgaatt
    15481 taaaaaagta tgtgccaggg tattaaaagt taaagagata gagatcttcc cttaagattt
    15541 tgatcgatga atataatgga ttacgttcat ttacagatcg caatcatatt tgtttgctta
    15601 tttttatcag catttttctc gggttcagag actgtattat tctcttttaa aaaaagtgaa
    15661 ttgataaagt tttctttttc tgaaaattta ctagaaaaca aaatagcaaa atttttatct
    15721 catcctcaaa atattttgat aacattatta acgggcaact tatttgtaaa tattttattg
    15781 tcatctcttt caacatcact gttattatcg caatggccac agtatgggca tattatagca
    15841 atagcaatag ttactcctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta
    15961 tccagcatca aaatcattaa gcgaattact tgaagaattt ttaacaaata aaatccagat
    16021 agcagttgtg gtagatgaat atggtggaac agcaggtatt gttacattaa aaagaataat
    16081 tgcagaatta tttggcagag gttatacatt gtgggaagac attcccaagg ctgaaataaa
    16141 aaaagaaaag gatgtggtaa tagttccagg agatatgcag attgatgaat ttaatgcttt
    16201 gtttaatgtt ttaatagatt catcagaaag tgagacaatt gggggctata tcattgaaag
    16261 gcttggctat ttgccaaaac gaagagcttc aatcatggtt gatgattata ttttaacggt
    16321 acggtatgtt acaaaaaaca gaatccaatc aattgaagta aaaaaagaag agttatgatg
    16381 gaaatatata ttggatcaca aattcttatc ataattttta catttctttc tgcattcttt
    16441 tctgcaactg aaacagctat agtatcaagt aatattataa atgttcaggc acttgtcaat
    16501 aaaggaataa caagagccag gagtattatg caaataattg aaaatcccga cagtgcactg
    16561 gtagcagttc ttatagggaa caatatatct aatattggtg caactgcgtt tataacattt
    16621 tttgcaagca aagcactttt aatacatgat tccaggcttt tcatagtaac tattgcccag
    16681 actatattct ttttgatttt ttgtgagctt ttcccaaaaa ttgtttcacg ttcaaagcca
    16741 gaaaaatttt tactgcatac gttctggcta tttcaagtta tttcattaat gttcaggcca
    16801 ctggttacca taatactggc tttttccact gttataaaga acgcaattca tgaagatgat
    16861 tccaggccgt tacctataag ttcccgtgac gagattgata cattgtttaa agccgtagaa
    16921 aaagaaggta ttattgaatc gcaacactac atctatttca atgagatact ttctttcagg
    16981 gatgtgagag caaatgaggt tatgactcca cttatagata ttatctcagt tgaaaaaaaa
    17041 cagtctataa aacattgcat agatttaatt gaaaaaacca ggttttcaag aatacctgtt
    17101 tttgaggaaa gggttgataa tatcattgga tatatatatt atcaggatat attaaaaaac
    17161 aaatccgtga aaaatattga tcaaataata cggaaaccgt attttgttcc ttcttcaaaa
    17221 aaaatatatg agcttataga tgaaatggaa gaacaagaaa tatcaatggt ttttgttgtt
    17281 aacgagtacg gtgctgtaga agggttgtta actgatgaag atattgccga agaaattgta
    17341 ggtgagatac aaacaaggga tcatccacag gatgattcaa ttaaagagat agcaccatgg
    17401 aaattcagtg ttgccgggga cctggatatt gaatatttcc agcgacgttt tggaataaaa
    17461 attgaaaagc gagattttga aacattagca ggttttctat tatatgtact ggggcatatt
    17521 cctgaggttg gtgagaaaat acatttccag cacataacat tccagattga agaagcaacc
    17581 gataggtcta tagaaagagt tattgtatca atcccaaggc gaaaagcttc tattggacaa
    17641 gcataggacc aagatcgtct ttacctgcca gtatatgcac atgcaaatgc catataacct
    17701 gtcctgcagc ggtgccgtta ttaatgatga cacggtagcc tttgtccaat ttttcctgct
    17761 ttgctatttc atttattgcc aggaaaatat cactcataat ataactattt ttttcattaa
    17821 gctcatcaat attttttatg tgatccttat gaaccactaa cacgtggaat ggtgcctgtg
    17881 gatttatatc cttaaatgca tatactttat cattttcaaa aactttggtt gatggagttt
    17941 tccctgttat gatgttacaa aataagcatc ccatagatat actcctatct ataaaaattt
    18001 gttgtagcct gtaaaacaat aactaaaatg agtattataa aatttgcaat aaaaaaagtg
    18061 ctttaccgta aaaaattgta ctgcttatat cagggttaat cagatactat gcgaactata
    18121 aaggatatac actatctggt ctatccagag gaagagaaaa gcaacacaat tgttgatagg
    18181 tttttatcaa gcgcaataga taacaggata gcctatcata atgaagaaga attaaattcc
    18241 ataataaatg aattgcagat taatacaata gattcaaaag acgtaataat tttaaaacct
    18301 tttcttggaa gattttatca ggtttgccct ggttcaccat atatgatttg ttgcagatac
    18361 agggttatca atacctgttt taattgtctg tataattgct catattgtta catgcatgta
    18421 tatttgaaca attatggtat tgtgcaattt accaatactg aaaggctttt tgccgaatta
    18481 catgatttta ttaaaaattg tttgcaatca atggtctata gaatcggaac aggtgaattt
    18541 accgattcac ttatgtttga tgaaattaca ggcattggtt ctgaactcat acagtttttt
    18601 tcaaagtaca acaatatatt ttttgaatta aaaaccaagt cagccaatat tgatcatttg
    18661 cttgatatac cgtctaaagg taatgctgtc attgcatgga gtttaaacac cccgcataat
    18721 attgcatatt atgaacatga tactgccaga ttagacgaaa gaatacatgc tgcagtaaaa
    18781 gcaattgatg caggttatta tgtggccttt cactttgatc ctattatact gtatcacggc
    18841 tgggaaaatg attattataa tgttataaaa ttattgcagg aaaaaataga tcctgaaaaa
    18901 gttgtatgga tatctatggg cggattcagg tatcatttgc atttcagaga gatattaaga
    18961 gataaatttc ctgaagagat gatgactgtg tatgaaatgt ttcccgggat tgatgggaaa
    19021 taccgatatt ttaaaccatt aagaatacaa atttataatt ttatgtatca acaattacat
    19081 gaaatattca aaaaggcata tatttatctt tgcatggaag ctgattatat gtggcaagag
    19141 ataacaggta agggttttca ttcatcagaa gaacttgaac aggatatatc taatcatttg
    19201 agctatcact ttctacaaaa aaataccatt ttctattgac catgcgctat aatacatatt
    19261 tattgtaatt aataattatt ataattaaaa acaaattcat attagcaagg agtaaaatgt
    19321 tggaatatac ggacgtaaaa gatttgatgc gagagttgac gcagattaaa gccagcattg
    19381 aagagctgta tactgcgctt catatagatg ataaaaagaa gcaattacaa ttattagttg
    19441 aagaaacgta taaagaaaat ttctggcagg atcaagcacg aagtactttg gttcagcaac
    19501 agataaccca gcttaaaaag aatattgaac cctgggatat tctaagaaaa gatatagaag
    19561 attccataat gcttttggaa atggcaattg atgaaaatga tactgatgtt ataggtgata
    19621 ttgcatcgcg ggttgagcaa tttacaaaac gctttgaaca attagaaaca ctggaattac
    19681 tatcagatcc tgatgatgac aaaaatgcca ttatgaccat tcatcctggt gctggtggca
    19741 ctgagtcaca ggattgggca agcatgctca tgcgcatgta tacgcgttgg gcagaagata
    19801 acggttttga tgttgaaatt ctggattata tgcctgatat tgaagcgggt atcaaagaag
    19861 ttgtcatgtt aataaagggt gaatatgcct atggattgtt aaagggtgag cgaggaattc
    19921 acaggctggt tcgaatatct ccatttgaca gcaaccagag gaggcatact tcttttgcat
    19981 cagtagatgt tatccctgaa gcacctgagg atattgatat tgagataaat gagtcagatt
    20041 tgcgaattga tacctaccgc gcatcaggtg ctggtgggca acacgttaac aggactgatt
    20101 cagcagtgcg aattactcat atcccaaccg ggattgtagt ccaatgccag aatgagcgtt
    20161 cacaacataa aaataaagcc tatgcaatga aaattctaaa atcacggtta tatgagctta
    20221 gaaaacggga actggaaaaa gaaaagttgg aaaaatttgg cgaaaaaaaa gatattgcat
    20281 ggggcaatca aattcgttct tatgtatttc atccatatac catggttaag gatcaccgta
    20341 caggggagga aacaggctca gtagatcatg taatggatgg agatataaat agattcattt
    20401 atgcttattt aaaaagcctt aaaagtataa aaattaaata gcttatttcc atttttttat
    20461 tgcataaatc aaaatcaggt tatgtagtat ttatatattg tacgaactta taattggtac
    20521 atggtcatac tctttattat taatattaaa tgctatcagt ttgttcagtg gcattgtatg
    20581 ttgtttgtga tattgtgatt atatgataca tactgaaaaa tatactattt ttttcttaat
    20641 aatactgtta ttatttattt ttactcccca atatacatat gcagcaggtt caggaggaac
    20701 ttctggtgcc gattttttgg aaatcggtgt tggcagcaga ccgcttggta tgggtgaagc
    20761 atttactgct gaagttggtg atgtaaatgc tatttattac aatccagctg ggttgggtac
    20821 tctgaagttc ccggttatga gtttaatgca tcaagaatta atagtagatt caagatttga
    20881 aaatgtttca ttagcatttc cagtatggaa tggttttttt ggtttttcaa cttcagtttt
    20941 ctgggttccc ccttttgata aaattgatat taacgggaat aagattggaa cagttaattt
    21001 ttacaatgtt agtggcatcg tggcgtatgg ttattcctta ggatttatgg aagtaggggg
    21061 atcaataaaa tatatctatc aacaaattga tacattgcaa ttgcaatcgt ttgcatttga
    21121 tgcaggaatt ttgaaacgta taacaatgta ttctcctttt gatgctccaa taagaaattt
    21181 atctcttgga ttatcaatac aaaatattgg aacaaatgct aaagatgata gtctgccacg
    21241 attaatccgt gcaggattat catataaaat gtcacatttt tttggtttta atgttgattt
    21301 gatagaaaat gctattacat catctgatct gtatgacttt acttcaggat ttaatgaaag
    21361 ctttagaatc aatgcaggtg ttgaattaaa ttatcttgat attttatttt ttagaggtgg
    21421 atatagattc aatgatgcag gtaaatatac atttggcctg ggttttaact atgcaattaa
    21481 gaacattgca tttgttatag atgcttcata tcaggatgca ggtgtatttg gccctattta
    21541 ttcttttacc attacattta aacttatacc aaaggtcatt actatagaag acaaattaaa
    21601 agctgaagtt cattatcaac gaggtatgaa atattttatt acagatgata tagatggtgc
    21661 tatcgaagaa tttaaaaaag caagggatta taatccatat cataaaaatg tagataaaaa
    21721 aatacaggat ttagaagaac ttaaagagtt aaaaaggcaa aatgagttac ttgagaaaga
    21781 acaacagaaa atacgatgaa atacgtaaag taactgtaat accacaattt tgtcctgctg
    21841 cattgggttc agtattatac acatgtggta atacacaggt aatatgtgct gtttcacagt
    21901 caccgcaggt accacaacat gccatggata aaggtattgg ctggctaaca gctgaatata
    21961 cgttattacc gtatgctaca aatccaagga ctgagagaag aaaccttcgc ccagatggga
    22021 ggtcagttga gatccagcga ttaattggac gaagtttgcg tacggtttta catttagaag
    22081 ctatgcaagg tgtttcactg gttgtcgact gtgacgtact tcaggcagat ggagggacaa
    22141 gaactgctgc aatatctggt gcttatattg ctttacagtt agctgtcaat tcattactaa
    22201 aacaaaacat tattgctgaa aatccagtca aagcccaggt cgcagcaata tcaataggct
    22261 atattggaaa agagttgtta ctagatctgg attatgaaga agattctcag tgtgatgtag
    22321 atatgaattt ggttatggac agcaacaata atataattga gattcagggt actggcgaaa
    22381 agcgttcata ttcaatacaa gaacttgaat caatgttgca attagcacaa gatgggatac
    22441 aaaagatatt caagatacaa aatgatatgc ttaaattgtc attataaaag ccgaatataa
    22501 atataatact atatcttatg tatcaattca tgaaatggtt gggataacta tgcgaaaaat
    22561 aataattaca atacttttta tcacttttac acatcatgtt tatgctcagt attatataag
    22621 aaatacaaca gtgattgaca caccaactgc atatacaatt gcaagaggta cttaccagtt
    22681 atcatttctt gggtatgata atggcggggt tgagcttaaa gcatttattg ggcttcataa
    22741 taattttttt ttgggagctt cgctggatat tcagaatgca attggtaaag atgatcctga
    22801 tcccaatgta cctggtgtgg tagggaaaat taaatttact gatggatggg aaatgttccc
    22861 aatttcattt gccattggct atgattcctt ttatattggg gaggaaggca agacatataa
    22921 tagtaagaat gaactgaatc aaatgatata cgggccgtat tttgtaatta caaaacccat
    22981 atatttattc aatgatgaac agcatataca ttttggtatg cgtataccaa ctcagcctga
    23041 atatgtccct gaagatacat cttatttttt agctctggat atcccacttg gagaaatatt
    23101 tatatttaaa gctgagatgg aacgtgtata ttataacttt gatagagctg atgagtggtt
    23161 gtacaacgtt ggtatgagat attcgtacat gcaacgattg ggaatagaat ttgatgtatt
    23221 attccagaaa gatgaaaatc caaatcggat tatacgtatt gaatatataa atgaatttta
    23281 aagttataaa taaaaagtaa atagtgaaaa tatttaaaaa aaacagtata tttgtattga
    23341 tactgcttaa cgcaatgtat tttgttattg tgcagaaata ttattataat ggaaagcttt
    23401 ttgtttatgc acaaaataaa aaaaattgtg ttatatacat tattaataca ataaatagac
    23461 ccataaattg taatgttgag atagctgata cgggtgaaga gcatgctaaa ggactcatgg
    23521 ggagatcaga attatgtgat aattgcggga tgctatttgt ttttgatgat gaggattaca
    23581 gaacattctg gatgaaaaat accggcatcc ctttgagtat tgcattcata gataaaaatg
    23641 gaataataaa tgatattcag gatatgaaac catatcaaac tttcccaaca tactcatcaa
    23701 aataccctgc taaatttgct ctggaagtaa atcaaggctg gtttaaaaaa aattttatta
    23761 caaccgggtc aaaagttata tcaaatggat gcatcagtaa atagaatccc catttctgaa
    23821 aatgaaaaga ctattcttat aggaatacat ttaccaaacg ataatgaaga ggaagtgttt
    23881 cattccttag atgaattaag acaactggta ataacagcgg gtgctgttgt tgtttctaag
    23941 ctggtagtta aacggatttc actagatcca gcatatatta ttggtagcgg taaacttgaa
    24001 gagataaaga aaatagttct ggaacaatca atcaaactgg tggtttttga tttaaatatt
    24061 atccgccctg ttcagatacg aaaccttgaa aattttcttg gttgccgtgt tataggtaga
    24121 actgaggtta tactggacat ctttgcacgt agagctaaaa ctgtcgaatc aaagattcaa
    24181 gttgaacttg cacaacttaa ctatatttta ccaagactta agggtcttgg aggtgtttta
    24241 tccaggttgg gaggtggaat tgggacaagg ggtcctgggg aaaaaatgct ggaaactgat
    24301 aggcgacata ttgttaaaag aatacaaacc cttaaaaaga aactatcaca catatccatg
    24361 cacagaaatg tgcagaggca ggagcgtctg caaaatgaaa taattggtgc agtagttgga
    24421 tataccaatg cagggaaatc aactcttgta aatatgctcg caaaggatga cttatttgtt
    24481 gaggataggt tgtttgctac tcttgatgca tatacccgaa ttgtctatct ggatgaatgc
    24541 cacaaagtct tgttaactga tactgttggt ttcatcaata atctcccttc acatcttgtt
    24601 gaatcattta agtctacact tgaagagata aaatattcag attttattat acatgttgta
    24661 ggcctggata tagatatcga aaaagctata aaggttgtga atcacgaatt acaattattg
    24721 gattgcagta ataaaccagt tatactctat ttcaataaac ttgatattgc aaaagaggaa
    24781 acaatccagt atgtgcattt gcggtttcca gatgctatac ttggttcaag tttaactggc
    24841 cagggcattc aattattgaa ggaaagaata atagaaataa ctgaaacatt agataaacat
    24901 aagcaaaaca tttcagtgta ttcataaaaa atcaatcctg gtaaaaatac gattttattt
    24961 atatcattca aaacgttatc cagtatctat tcctatattc atatcccaaa aggaacctga
    25021 aataaaccaa ttacaggatt tggaagggct ttagatgtga tttttaatta gttatattat
    25081 acaattatta tctaattgat taattctata gctggcgtat gtcgattaat ataatacatt
    25141 gctactggaa gtttaatatt ttttagggtt gatgttacaa tgtatgattt taaacgatat
    25201 tccttcccgg aagaaaggaa aaagaattct tttatttatg gttttttttc actgttattt
    25261 attcttgtga tagtaatatt taccctgatt gctgataaac cacagaataa taataaaaaa
    25321 attgcttcat caaatctgtc atcagttgaa aacaagctta ttgttgatgg cattaagatg
    25381 gaagatgata tgcctccagt tgaaactgaa ggtgaggtta tatttgatga taattttgat
    25441 gatgatatag acctatcaaa taagattgtg gtagattcct caaaaagtaa aaaaaaagaa
    25501 aaaagttata atattgaaga attgaaaaaa agagataagc gctggcatca tacaaaatat
    25561 caaatccaaa aaaatgataa tatatggaag atagctcaaa aatttgatac aaattttaag
    25621 ttaataatat tggccaataa tataaataat ccagatattc ttaaacccgg gaggaatatt
    25681 atagtcccta acagaaaagg ggtttttcat aaagtaaaaa aaggacagtc gctaattgct
    25741 atagctcgtt actacaatgt ccctttacat gccattattg cacataattc gttagaagat
    25801 cccaattgta taattcagga acaaactatt tttattcctg atgcaaaaat tgatttacgt
    25861 gataaaaaag ataagcgcaa tatagcaaaa aaagaacctg taagcgttat aaatcggata
    25921 aaatcacaga tatcgttaat gtggccggta agtggcaaaa ttacatccgg ttttgggacc
    25981 agaagaaatc cattgggcaa aggcagacaa tttcactgtg ggctggatat cagttgtaat
    26041 gttggtacac cggttaaagc agctttggat ggtaccgtaa tttatgcagg ctggaaagat
    26101 ggttatggaa atgtagttgt gttaaaacat gataagggat atatttctgt atatgggcat
    26161 aatagtaaga atttagttaa ggaaggtgat agcgttcaaa agggacaggt tattgcaaaa
    26221 agtggcatga cgggttctgt aaccggagca catgtacatt ttgaattcag aaaatatata
    26281 acgccgctta atcctttacg atatttaaag gaaaaataat gggattcatt aataatcata
    26341 ttctccacat ttttaatcgt tcaatttgta tcgtattttt attagtgagt actatatttg
    26401 caaccggcca gatgaaatat gataatcctg gccagattat tcctgttttt tatacacaaa
    26461 atgattatgt tgcatggaaa gcgatagtta aggggagaat tatcagcatt ggggaaaggg
    26521 aagatacagc aaaacaggaa ttaacccgca cagtccagcc taaaacaaaa gtaactgtca
    26581 ggctttataa tactgacggt atccataaag gaacaacgtt atatgtgata aatactaata
    26641 atttaattgt ttcccgtttt acggttgaaa ctattttccg ttcacaatca tttggcgata
    26701 tgctggttgg gtatggatat ttcagattat caacaatagg tgatagggtt attcaacgat
    26761 ttgaaggtgc atatcctgaa tttgcaaaga ttcataaagc acggggtgat tattataaaa
    26821 acaccggtga atttggcaat gccataaaag aatataatca ggcaattatt cttgatgcca
    26881 gctatccgga agctcatatt gaactgggta aaatatattt taatgacaat gttttacaat
    26941 ttgcttttaa tgaatttgaa caggcataca aaaataaaga caggatagtt gataagcaag
    27001 atatatatga gttattatat tacatggcaa aaacacgatt tatagaagca tatgaaacaa
    27061 atatacctgc ttcactaaaa gaaaaatata ttaaagacgg cattaaatat gcaaaagaag
    27121 cattaatcat tatgcctgat tctattgaag ttttgtatat gcttggaatc atgtattata
    27181 aacaaactga tccagatgat gttgaagcta agaagatatt tgaaaaagtt attgagttag
    27241 atccaacaca tactggtgct tatatagcgt tgtctgaatt atacatgaaa cataaaaaca
    27301 ataacaaggc aaattattat tctaagaaag ctttagaaat agacccggca aatgaacgtg
    27361 cgcgatttat atatagaatg acaaaagaaa agtaattaaa tatattcagt atgtgatttt
    27421 ttagaaaacc agattaaata ctgcaaatat acttgactat tatcaatact gctgcataag
    27481 ctggttaccc cacattatta tttgctttta tagggtagta tgtatggtta aatttttgca
    27541 aaatgaaatt gtgcgttcac tattgccatt gattatcatt aatgcactgg ctttattaag
    27601 ttttattgtt tttttattta tatatcctaa aaggcctaag gatcctgaaa tagtagcaag
    27661 aatgcatgag acgtttatgg gccttatttt cagggaattc tggtattggg ttaatcagcc
    27721 atttattaat ttctttattt attttaaaat aaaacccaat actattactg caatttccct
    27781 tatactggct tttgtcagtg cttattttta ttatattggg aattttggtc tggcagggtg
    27841 gatactcata gtaagtgcaa cactggatat aatagatggc cgtgttgcac gaacaactaa
    27901 tacagtaaca aaatcaggtg catactggga ttcatgtgtt gatagataca gtgaaggtgt
    27961 tgtgttttta ggcatagcgt tgtattatca gaataatttc attgcattac ttgccacaat
    28021 agtagcttta ataggatcag aactggtaag ttatacaaag gcacgaggag aagctatagg
    28081 tatcactaca aaacggggag taatgcagag agctgagcga ttaacaatat tgtgtgtcat
    28141 ttcagtttta cacccattct tccaggtatt attcaaaaat tcatcggtca atcctgaaat
    28201 cgttatgatt ggtgctatga ttcttatggc agttactaca aatcttactg cagcaaccag
    28261 aatgcgaatt attttcaggg agataaaaaa aaccgagagc aatgtatgaa aaaacgtttt
    28321 caataaagac gttagggtgt aaatgtaatc agtatgaatc tgctcagata gcatcacagt
    28381 ttttatcttc aggttttaaa gcagtcccat ttggctctcc tgcagatata gtaatcatta
    28441 acacctgcac agtgacagat aaaagcaata aaaaatgccg taattatatt cgccagggtt
    28501 caaaattttc aaaaactggc aaggtcattg ttacgggttg catggttgaa acacataaag
    28561 atgaacttga tgccatgaat gaagtacagg catcttttac caacatgcaa aaagataaac
    28621 tggttatttt atcaggggca attggtacag ataatgaaaa cacttccata agtactattg
    28681 caaatgcgta tgcattgcca tttatgcgaa cgcgtggcta tatcaaaatt caggatggat
    28741 gtgatggggg atgctcatat tgcattgtcc caaaagtgcg tggtaatcca acatcaaggc
    28801 cggtggatga tattgtaaaa catgcacaat ttttaattga acgtaattgt cctgaaattg
    28861 tattaactgg tataactatt ggtaaatatc agtataacga tgatacatta gcatcgctta
    28921 taaagaaaat aattactctg gagggtaatt tcagagtacg agtaacgtcg gttgaaccta
    28981 cacacgttag tgaagaactg atagatattt taaagcatga taaagtttgc aaacacattc
    29041 acttaccgtt gcaatcaggg tctgatcctg ttcttatgca catgaatcgg ccatattcag
    29101 tagatttcta tacaaagctc attgatagag taaggacatc tatgccagag attgctattg
    29161 gtactgacat tatcgttggc tatccaacag aatccgatga agattttgaa gcaacggtaa
    29221 cattgcttaa gcaaatatct tttgcctatg ttcatcagtt cagctattca cccagggaag
    29281 ggacactttc cagtaactat cgcccattgc catatgatat tatccatgca agagcacaac
    29341 gtttgcgcga tattgcacgg gaatgttcat accagtatcg tttacagttt ttaaatacaa
    29401 ttcgtccagc agtcatagaa aaagatgggg atggtataac ggcacttacc gataactatc
    29461 taaaagttgc acttgaaaat aatcattata gtagaagcaa gttgggttcg atagtacctg
    29521 tcatgataac atcggtatct gaagaaaaaa ttgaaggttc gatagaactt tgaactcaga
    29581 atgttgcatc aaatgttacg gtgggcattt ggcgcaaatt gactggaata gcgatgttga
    29641 aaggtatcgg tcacctctgt cgggtaggat agtaaccacg gtaccctgtt tcagatcgtt
    29701 tgcaacctgt atgcttcctg caacggcagc accgcttgac ataccacaga aaaggccttc
    29761 ctgtaatgct aatttgcgtg tatgttcaaa tgcggtttca tcgtcaatga taatgatgtc
    29821 atcaagcatg ccgggattat atatgtcagg aactatcgcc tcctgcatat tctttaaacc
    29881 ctgaattgaa tgacctggga caggttcaac gccaattatc ttgatttttg gattatattc
    29941 ttttaatctt ttggctacac ccataatggt tcctgtggtt cccagtcctg ctacaaaagc
    30001 gcttatctgc ccgccagttt gctccagaat ttctgcgccg gttgtttcat agtgcgccag
    30061 tacatttgcc ggattggaaa actgattggg cataaaataa acatctggat tttcttcata
    30121 cattttatgt gcctggatga ttgcgccatc agtacccatg caaccttcag tcaacactaa
    30181 ctgtgctcca aacgcaataa gcgtctgctg gcgttcaacg ctgacacatt ttggcatgca
    30241 aagaacaact ttgtatccta atgcagaacc aaccattgca agaccaatac cagtattccc
    30301 acttgttggt tcaagtatag ttttgttggg tgttaatgtt cctgattcaa ttgctttttt
    30361 aatcatgtac caggcggtac ggtcttttat ggaaccgcca ggattggcgc cttcaaattt
    30421 tgctagtatt ctgcttcctt tgggtgaaag ttttgataac tcaataagag gagtattccc
    30481 tatagcagca ataagggagt gggaagtaat actggtaaaa tgggaattag tcttttgcat
    30541 ataaataact gtgaataaat ctaatggcac acaatactaa atgaataaat atgtcaattg
    30601 ttattatatc gccataaaaa ggaaatacat aattttttat tatgggcgat agtaactgtg
    30661 gaagggtttt aattaaagga atgagcgggt gatgggactc gaacccacgg cgttcagctt
    30721 gggaagctga cattctacca ctgaattaca cccgcacttt ttcctaaagt agttctaata
    30781 taagtttttg caagaatttt ttaagagtat atatggattt ttcaaatttt ttaaatttac
    30841 aataaatcta aattatggaa gtttataatg aaataaattt attttttcat gcaacaaatg
    30901 tctggttatt tctaatgctt ttgtcagcag gagtatataa ttcatttctg ataaaacatt
    30961 tttagtaatt attgctgatt gtggtaataa ccaactattt ttttccatac tgattagaca
    31021 caggataaaa aattataatc ctgattaata ataaattagt atttactata caaatctatg
    31081 gatatttata tataaaatga tatattaaaa ttgtaaatta tatactggga tttttaatat
    31141 attgtattct atgatggcat attatgaata tatattgtta aaataatagc aataataata
    31201 taagattttt ataataatag cacttgttgg taaaaatgcg ttaaatctaa atttttttat
    31261 tgaaattaaa gttccatatt gaaggattgt actgattact atttatcatt atattttttg
    31321 cgtaacaatt attaaattta tactaatcat aaattaatta ttaaatatca aaaagaggta
    31381 atatggcaag cttagatgcg gttgttaaac aggttgtttc aaagtac
//
LOCUS       DAORYL010000029        31176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1220_length_31176_cov_12.961280, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000029 DAORYL010000000
VERSION     DAORYL010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 31176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31176
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1220_length_31176_cov_12.961280"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PLV81_07130"
     CDS             complement(<1..689)
                     /locus_tag="PLV81_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOM09909.1"
                     /translation="MDYKYYLIEKKAPIAWVYLNRPEKKNAMNPPAWWEAPSVFKELD
                     ADDEIRVIILAGKGPAFTAGIDLIEMAGEIKELADPAQKGGIKRSLLQKIYKMQDTMT
                     CIEKCRKPVIAAVHGYCVGAGLDMISACDIRLATADAQFSLREAAVGFVADVGVLQRL
                     PHIVGQGITRELAYTAKYITAQRAKEILLVNEVYPDQKALYEGAEKMAMEIAENSPLA
                     VQASKDVLNFG"
     gene            1066..1884
                     /locus_tag="PLV81_07135"
     CDS             1066..1884
                     /locus_tag="PLV81_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09910.1"
                     /translation="MFLSIFLLPFSSQAQVNSGDFGLILNGGVGTGSSMYGVILNTTN
                     DSSSTLGVGPGSSFNAGFMANFNIIAIHSQLYYVTLKDLEWEQEVSGVDRKFKTEGSG
                     HFWTWDGTIGIKALTEEGDMGYTHVYVGFRIWKVLREEDKKTVDGVSNPLYPPVKQEL
                     TGNGFIVGIRDFSTLPLGSISLALQTGLWLYKAPLSTFKQDGTEIDTTADQNIGFGFE
                     IGGGLAFEDIGLSTIVGLRMDVQATEIKLPLNIDAVAGSGYAQFFFAVSKEFGF"
     gene            complement(1947..2543)
                     /locus_tag="PLV81_07140"
     CDS             complement(1947..2543)
                     /locus_tag="PLV81_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09911.1"
                     /translation="MFKKLALCILVAAVACVTTGLQAQEMTDDVAFLRVGYQWNTLKP
                     DVANAEDIKLNGVAIEGEYNLNLGGMLLGFSLEYAYLSYKDDKAYDYGFLSPMVTLKF
                     LAPGGFYIGPGVSARYLMDSYLPKGQDEPDSEVDLWVNGVAGFMTPIAEGIYLDLQAR
                     FGWNLTQNQFEDIFTKIDKNYDMAFYVGVGTRTRSTGI"
     gene            complement(2685..2849)
                     /locus_tag="PLV81_07145"
     CDS             complement(2685..2849)
                     /locus_tag="PLV81_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOM09912.1"
                     /translation="MQELIKEGCFLKRHGANHDIYVNPSNGKKAPVPRHAEIKDSLCK
                     LIRKQLGIDK"
     gene            complement(2837..3067)
                     /locus_tag="PLV81_07150"
     CDS             complement(2837..3067)
                     /locus_tag="PLV81_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017839951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOM09913.1"
                     /translation="MTKTFTLEYWIDEGWYVGRLLEVPGVFSQGETLEELIVNIREAY
                     KLMVEEVEPVYIETKRKEIEVEVSKEKNLFRS"
     gene            complement(3168..3458)
                     /locus_tag="PLV81_07155"
     CDS             complement(3168..3458)
                     /locus_tag="PLV81_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09914.1"
                     /translation="MRISDGLSIITLPQTTAKPARTVMVWPVYDDSRITPVKGVARRL
                     ENNIQYIKPTPDEHARLLSQVQENPEPLYSNTARIQKSMSAVIPGTFFDAIA"
     gene            complement(3523..3761)
                     /locus_tag="PLV81_07160"
                     /pseudo
     CDS             complement(3523..3761)
                     /locus_tag="PLV81_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004494163.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
     gene            complement(3791..5575)
                     /locus_tag="PLV81_07165"
     CDS             complement(3791..5575)
                     /locus_tag="PLV81_07165"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HOM09915.1"
                     /translation="MKSLALSVCLLVTSLSYAWQPLLYQATNGKLYYTGKPYHQGLPD
                     TIHIKDIQVIKNTFYISTREHGLFKRILGDSSWQHITPRDAHIRTTNGLLPTYRSISA
                     FCVDGSDPSILYVATKYSLYCSEDGGTSWQSVALTGLPANACITALYAKGSTLIVGTS
                     WNGVYRYTGTRFINISNGLPRKHYSDSIVFIEEVYRVSPEFCITTYTNEVYSYNGKEW
                     KKRLSGHTGTFWGGSINGKVVVVDNDTVVTLTGQKEIHQELSPIQAHCVLFLEGTQYV
                     FVKAIEEQKDTVRGLYAGVPSSWKVVKELVAVAKKAGYNALVFDIKDDYGNVYVSEPT
                     AESIGALRKGLPPEEVCTYVHNHTMKAIARMVVFKDERLFKAFDTKYAIKDSATKAAW
                     RGNPKEYWVDPYAEFVHSYNIAIAKEAQRAGFDEIQFDYIRFPTDGPVHRCVFTYKPP
                     NGIYKYEAIAQFLRSARQALHVPISVDVYGFTVWYEFGQWLGQDIEAMSSIVHVLCPM
                     VYPSHYGRKFFEGNIDPGDYYAIVIESNRRALMKSHAVVRPYLQAFNMLSPGWGYTYI
                     QRQIEACTDSGVSGYIWWNAGRDYGVIK"
     gene            complement(5556..7238)
                     /locus_tag="PLV81_07170"
     CDS             complement(5556..7238)
                     /locus_tag="PLV81_07170"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOM09916.1"
                     /translation="MTKPLIVQSDNTLLLEVDNPLYEEVRDAILPFAELEKSPEHIHT
                     YRITPLSLWNAASAGLTAKEIVERIERYSRYTVPAIVITTIKEQMDRYGLLTLIKRGE
                     NLILQSDDVHIIREIATHKQIHKFIEEQLSEYEILVKPNNRGHIKQALIKIGFPVEDK
                     AGYVDGDPCPFELRSVTASGIQFTIRDYQKSAIDSFYRSGSDEGGSGVVVLPCGAGKT
                     IVGIGIMHLYQTKTLICVTNTVALRQWIDELLDKTTLTKDMIAEYSGDVKDIKPVTIA
                     TYNILTYRRKKEDEFKHFGLFTQNNWGLIIYDEVHLLPAPVFRMTAEIQSKRRLGLTA
                     TLVREDGLETDVFSLIGPKKFDMPWKLLEECNWIAKAICTEVRIELPESLRYQYTVAG
                     DRQKFRIASENEKKLHYVEQLLKHHPNDRVLIIGQYIDQLQKIQERFSFPLITGSTPV
                     KEREELYKQFKQGAITRLIVSKVANFSIDLPDANVAIQVSGTFGSRQEEAQRLGRILR
                     PKPGDNTAHFYSLITAHTVEENFAHNRQLFLTEQGYTYIIMNESMFNEKFST"
     gene            complement(7242..7673)
                     /locus_tag="PLV81_07175"
     CDS             complement(7242..7673)
                     /locus_tag="PLV81_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOM09917.1"
                     /translation="MIKVILSQNFNASAAEVFAALDHINKLARLKTDLRSYRIARDEE
                     GLQLIDVDVRFIVKQFPLRLKYTAVPHKYCELKMLKGALKQYEYRYTVMGDNVSATVT
                     VECSIRLPFRYFLVTPILRAILKRRIKKELLLLEKILHTTL"
     gene            complement(7946..9328)
                     /locus_tag="PLV81_07180"
     CDS             complement(7946..9328)
                     /locus_tag="PLV81_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOM09918.1"
                     /translation="MEYNVETLPLWKKIIYALGQLGWSLASFSVGNLLIYFYLPPESA
                     DKAIFPAYIYQGYVIGVLTIIGLIYAFGRLWDAITDPIIATMSDRSRSPFGRRRKFLA
                     IGAFPFAILGMLAFMPPVDTLSTWNAVWLFVVVTLFYWFMTMYVTPFFAWLSELGHNP
                     NERLFLSTLISITWAIGFAIGSQAVALQSLFEKSYGYSSTKAFQVVMIIFSGVSLVLM
                     YLPVIFIDERRYCEPNVSNEGTFEAVRSAFKNKNFLFFTISDLAYWVALTFITTGLVY
                     YVTVLLELDKAVYSQYMIILFALSFIFYVPVNLIAQKIGKKKLLVFAFVLYSSVYVVA
                     SMLGWFPIDKNVQGLIIIVMTSLPLAIFGILPNAIIADIAEADGIQTGNFKAGIFFGA
                     RTFMSKMGQMIAALIFPSLILLGKTPQNDIGVRLTCVVAFFFCIAGLILFLKYDEKKV
                     LEILATKEKV"
     gene            complement(9719..11836)
                     /locus_tag="PLV81_07185"
     CDS             complement(9719..11836)
                     /locus_tag="PLV81_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOM09919.1"
                     /translation="MKTFFYPESMVVVGVSLTKINLGKIILLNNIQQGYTGKLFGVST
                     QEGRIEGVPIYKDILDLPEVPDVAIIITPAKTVPEQLEKCGKKGIQHVVIESGGFSEY
                     SQSEQSLEQQILAIANKYGIKIIGPNCIGTINFEKKMFMPFAFFRKDLVAGEVSMISQ
                     SGGVGNTYLHALPENHIFINKFVSIGNKLMLDEVDFLDYFVNHDDNTRIIICYLEGFN
                     RGREFFDIARASEKPIIVHKSNRSPLSAKIAQSHTTALSASDAVVDAAFAQAGVIRAE
                     DEEEVILAVKAMQLPPMKGRNVAVLSRSGGHAVISADACAKYNFNLITFPESYINTIR
                     NLYKTRVISHQNPLDLGEIFDYTIFGKIVEETIKLDEVDGILFNHLYQSEYEAGMSRT
                     FLDTVERLVREYNKPVMVAMISDAKEMLDINMNHPMPIFNTPYNAAHALAISADYYQK
                     KAMRSNRGIIDTTMMNSVKTQIPYFDTIGKENREPLLHESIQFCQRVGVPIVNGILVQ
                     SAEKIEKTEFQFPVAAKIVSPDAIHKTEVEGVKLNINTKEELKDVFDNMRKSLYDYNP
                     NAVFSGVYVHTMAPGGTEFFVGAKKDPVFGPIVLAGLGGIYIELFKDVSIRLAPVTKA
                     EALEMIRELKAARLFEGFRGKEPLDANAFSDVIVTLSHAIASIEEISDIECNPVMVYS
                     HGCMAVDARIFLQTQKVASPTSW"
     gene            complement(12087..13289)
                     /locus_tag="PLV81_07190"
     CDS             complement(12087..13289)
                     /locus_tag="PLV81_07190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOM09920.1"
                     /translation="MNKSIDDYRGIIDDSIISMLYSKANKLSKYHIVNINSTYQGGGV
                     AEILNSLVLLMNDAGVFTGWRILHGTPDFFAITKKFHNALQGDSIHFTKMKCQLYEET
                     NYNFAKFTHINHDAVIVHDPQPLPIIRYYRKRQPWIWRLHIDITNQNIKLWEYLKTFI
                     LHYDMMVISHEKYKKADIPIPQHVIHPSIDPLTPKNMQLPKAVVQKYISKFNIPIDKP
                     FIVQISRFDKWKDPEGVIKVFEKVKQEVDCRLILCGSMASDDPEGMVIYNKIFNKIKN
                     SKDIILYTQENNVLVNILQTQAAVVLQKSLREGFALTVSEALWKGTPVVASDVGGIPL
                     QVIDGKTGYLVDPYDIDGCAKRVVELLKNEEMRIEMGKNGKEHVRKNFLITRELMDHL
                     DMLITVLS"
     gene            complement(13282..15501)
                     /locus_tag="PLV81_07195"
     CDS             complement(13282..15501)
                     /locus_tag="PLV81_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HOM09921.1"
                     /translation="MRLVIVSNRLPVTISESGGTITFKESVGGLATGIKSYVDSLQKA
                     MSEKVEYIWIGWPGPMDNLEHLVKTSEITKTLVDSYNCYPVYIEEELIDKFYYGFCNK
                     TIWPLFHYFAEYTIYNDDYWDAYKYTNNMFYKAVMSILRPDDIVWVHDYQLLLLPGML
                     RKSINNAIGFFLHIPFPDYEIFRLLPKKWAQEILMGILGADCVGFHTYSYTQYFLRCA
                     LYILGYSNDMNKIIMEDHIVKVDAFPMGIDFHKFNNAIKLPEVRKARESYMKKLPDVK
                     IILSVDRLDYTKGILNRLYAFEKFLQRYTEWHEKVVLLLLVVPSRTEVEHYNEIKRQI
                     DRMVGKINGEYGTVKWTPVIYLYRSEPFYRLVALYTISDIALITPLRDGMNLIAKEYI
                     ASRFDKTGVLILSEMAGAASELGETITVNPNDINDIVCAIEEALIMTEDEQIKRNVIM
                     QKRLERYNVVKWATNFIKEMLLVKAEQEKFNSLLLNSEKEKEIVDAFKNAKKKVLFLD
                     YDGTLVPFEKQPELAKPDEDILNQLKLLSNMNNTDVVIISGRDKDTLEKWFGTLNVIL
                     IAEHGIWIKENGLDWKLNITPADDWKPTINQILEIYVDRLPQSFIEEKEYSLVWHYRK
                     SDPEMSSLLAKELTDYLISFAANMNLQIMQGNKVIEIKNSGMDKGTAALSIISKNDYD
                     FILAIGDDWTDEYLFKALTEEAYTIRVGIIQSYARFNIRDYQSVRKFLSKLKEQNYE"
     gene            complement(15561..16619)
                     /locus_tag="PLV81_07200"
     CDS             complement(15561..16619)
                     /locus_tag="PLV81_07200"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOM09922.1"
                     /translation="MIESVLAKTFFGNSVRDYCISVLIFIFALCCIFIFKRIIIRKLK
                     SIVQRTNTVFDDIIIRAFENSFLPVTFIISLYVSILQLSIEPVVRKAINICFLVFFTI
                     SGVSLVVKGVGIFISVIIQRQVDTTKQRALRALNPLLKVIIWIFASYFLLENLGIKLT
                     PLIAGLGIGGLAIGLAAQNVLGDLFAYFVMLFDRPFEIDDFIIVDTDLMGTVENIGIK
                     SSKIRSLGGEQIIISNSNLINARVRNYKRMHRRRVVFRIGVTYETKIEQLKMIPGLIK
                     EIVSNIDNATFDRAHFFSYGDFSLQYEIVYYVESNDYNLYMDIQQEINFKIKEAFEEH
                     EIEFAYPTSMVYIAKDIN"
     gene            16961..17542
                     /locus_tag="PLV81_07205"
     CDS             16961..17542
                     /locus_tag="PLV81_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOM09923.1"
                     /translation="MRKKENTEKIFSAALKVFSKYGYKKTTVEDIAKELNMTKGNLYL
                     YVKDKKDLYHKVVEFALLRWQNYVRQAIHNESDPRNKFLTMCHKAVEYLSIDAEFRNI
                     LVHDPEIFPMFTNKDPYERINNNSVTMIKNILKEGIGKGLFRPVDISAMSKAIFSIYK
                     MFVIRMYIRSEEKDVRSMFEQTLELITQGLFKQ"
     gene            17579..17980
                     /locus_tag="PLV81_07210"
     CDS             17579..17980
                     /locus_tag="PLV81_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOM09924.1"
                     /translation="MPQELMYLTKEWRDEAERRLKNELTPEKMNFITSSMSNIYLNCP
                     DGKNKYLFFKFIDGKLDTLALGEGEPPDAEFRISGDYQVFAMISRAELGSQKALMTGK
                     LKLKGNMVKALKLAAIADRLNKVLSQIPAQY"
     gene            18019..19245
                     /locus_tag="PLV81_07215"
     CDS             18019..19245
                     /locus_tag="PLV81_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOM09925.1"
                     /translation="MTSRLENKGPYYIDFPGRIKAIQQEMAKEHIDVYLGSRLRTLSW
                     TLDAFCPWRSFVVIPNEGMPAAFTFVIDAARVADESWLDEDHVFGFVPMGGQDQIELI
                     AEYIKDLLKNKKGRIGVENGMSNYLPEGNLTHYEYERFKDALPDCEFVDAYTIIDKLS
                     MIKDEGTINRFREASRIVDVGHKAVYEAISNGGWKGMTETEIAGLAAYAMRKEGSEWE
                     WSYTGGNEIASGYRTGLAGGACTPATRKKLQAGEPLMVDIHAMFMLGLGDHSHNYLIE
                     PVTDRQRWHAQNFLDIVNLTLKTYRAGITPSLLAEQMMAFAEERGFADYMVPGFEHGI
                     GMMGDEWRIGLNDGPFPFWTNPDHVYQENELIICAMQYACPQDNIGFRYENPIVIHKD
                     GCEPLSKYPLTIDEIQ"
     gene            19249..19665
                     /locus_tag="PLV81_07220"
     CDS             19249..19665
                     /locus_tag="PLV81_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOM09926.1"
                     /translation="MAVITGYISIATQGQGDIIDITLDAQKIVTRNKIQDGLLCLFVP
                     GSTAAITTIEFEPGLQKDINLFLEKIIPRNAHYHHHDTWHDDNGSSHLRASMIGPSIT
                     VPIVDGTLTLGTWQQIVVIECDTRKRNRKIVAQIVY"
     gene            19818..20534
                     /gene="prxU"
                     /locus_tag="PLV81_07225"
     CDS             19818..20534
                     /gene="prxU"
                     /locus_tag="PLV81_07225"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_083777221.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:20064..20066,aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HOM09927.1"
                     /translation="MPEEFKPGCTRPTTSPPPQDIILQEQQNSSKKEEHTMIQVGQKA
                     PEFSLPAYFKGKFVEVKLSEYLGKWVVLCFYPGDFTFVUATEVSAVAEKYPEFQKLGV
                     EVLSASVDSVFVHKMWNDNELSKMVKGGIPFPMLSDAGGKVGTMYGVYNSNAGVENRG
                     RFLIDPDGVIVGYEVLTPPVGRNVLETIRQIQAFQLVRESKGTQATPSGWKPGKDTLK
                     PGPDLVGKVWQVWTVDKAFD"
     gene            20630..21571
                     /locus_tag="PLV81_07230"
     CDS             20630..21571
                     /locus_tag="PLV81_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOM09928.1"
                     /translation="MSYTAYCFHPHYLHHKTGDSHPESPKRLEILDKYIRTNKNYSSL
                     LYVQPRKADPAVLTYAHDADYVTMIASAKPNGITCIDGYDTVICDETYDVSLWAIGGI
                     LEVCDYILAGEATNGFCAIRPPGHHAEIAQAMGFCIFNNVAIVARYLQYTYNIRKIAI
                     IDWDAHHGNGTQHIFETDPSVFYISTHQFPFYPGTGSVDEIGIGPGYGYTINIPMNPG
                     SNDKTYKSAFTEIIIPALDHFKPEFILVSAGFDAHKSDPLSSLNLSSGAYYMFTRMLM
                     DIATRYSNNRIVVVLEGGYEIQSMIESVDYVLKALME"
     gene            complement(21572..22648)
                     /locus_tag="PLV81_07235"
     CDS             complement(21572..22648)
                     /locus_tag="PLV81_07235"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOM09929.1"
                     /translation="MKKSAIFIVVLFYVYELSCTTPEERIPKHYIATSSDYSMLFIGD
                     MMFEWAVKDTMQRYGTEYPLKRIKPFFQSFDFVMANLESPLTRSSQSLVYQKYIFKAP
                     PSIAFILAEAGITAVTLANNHMLDYGVTGLLDTLTSLSHAKVLYAGAGNNEITASLPV
                     MRQLGTTRIVVLCYTQIASKEMIAKNNPGINFFELKKAQEDIKKYRFCDCVIINIHWG
                     NEYFYYPSSKQIDIAHALIDAGADAVIGHHPHVYQGIEIYKNKPIVYSLGNFLFGSVH
                     EGINDNIACALYMSGNGKIIKMHIYAIKGIYNDGSINIQPEVLEGTIAAKVFDHVLEI
                     SKPLGIIFTSRTTKHHNYAEFIFR"
     gene            complement(22652..23332)
                     /locus_tag="PLV81_07240"
     CDS             complement(22652..23332)
                     /locus_tag="PLV81_07240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOM09930.1"
                     /translation="MSDILVAIIVRNNEELLRRTLDSICNCPYAHVMPLIIDDGSFDQ
                     SPSIVSDVDVHFVMHEEPLGYGGALMSAFAYAKDYSLSSLFMLPLEAFADWHTVSGYL
                     SFFQDADIITGNRFTGQTFNEKKQYNDIVQYFNQHAGLNIVDPFSPLKGISMNHADLF
                     EITEFSDAALVQILIQAAHFKLRIKEFECMYPEKNLLSHLDDIENLPGLKDFITGELH
                     LYPYTKGH"
     gene            complement(23325..24128)
                     /gene="larE"
                     /locus_tag="PLV81_07245"
     CDS             complement(23325..24128)
                     /gene="larE"
                     /locus_tag="PLV81_07245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOM09931.1"
                     /translation="MTNQKIDILQKKLQALKPYAIAYSGGNDSTFLAAISKHFSMDFC
                     LIHCISEFTIPGDTQRGRTFAREHDIPYQEITLAVLGTVEIVTNNKDRCYYCKKAVFK
                     KIQEYAYRSGFQNVVDAGNVSDTSDYRPGRKALIELGIHSPLLEAGFTKEDIIQGLQH
                     LKIPYSKASNSCLATRIPYGTGITQDILDAIQRAEEFIISLGFDGVRVRYHFPVARIE
                     VMRQDMPRIVDPEIRNKIVAFCKKLGFLYVTIDLEGFTSGNLNRMLNHE"
     gene            complement(24200..25750)
                     /locus_tag="PLV81_07250"
     CDS             complement(24200..25750)
                     /locus_tag="PLV81_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09932.1"
                     /translation="MRNTVILYLLIYISILLQLEAIALQSISVEYRQDALDYGVKKEH
                     YLRTSATIATDTTEVNAAVVQSHDATLATWSFATQGDYAAIAGGYYFIHNATGLLLGK
                     TSVYNPDPYSIHVQKNDSFVSLCKSGNPQYAMYGVVSSLFDARGASTIKLDAGVSYKE
                     CYISSGDAQNGVYPYSVQGLLSHCKREGQYTEPVQVVTSFVHATLSPVEHVTLQGCYY
                     STHVYYNNAHMLFNANTQDNYAMESFGGYSLFAHYNDSIVSLFSEYAISHVLIKNEGK
                     SGNRYSSAYYCGVIVKEKNYKISSIVQKSDENFFAPFGNTFGGNSPRDMYYYSVSVKP
                     VKKFTCSWIYMDQNNLLPSPYYTEYPHKRINNIRMVYINKYFSVKSDYRYAQFYKDGE
                     ERASRYQQGVVWGITKNLSIHLKYGLYQGDTVAWYTASGIGIKVGNLQNDLGSVYAHT
                     DGEKMYVAMLPLPQTNIISETIAASSFFVVARLRFSNTYCKLSARCVMQLHPQKQTAG
                     EVGAAAWF"
     gene            complement(25735..26973)
                     /locus_tag="PLV81_07255"
     CDS             complement(25735..26973)
                     /locus_tag="PLV81_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09933.1"
                     /translation="MASLLVSLLLMLQSFETAQPLALNVWAIAPAYQANPSPLISGVT
                     YANPFGYPDLYRTTTFIHTAMHGYSVGLQWQYFGIPEYREDTLNVGACWAIAQWISVG
                     VEFHDYILSIHTETFSHTQSLYDYGMYCTIKPFERVSLFVMQNNIKNFHDDGYIPSET
                     ILSARAEFFKGCTVEYEFHYGDYISQIVKINGYITRYCAVDIGYSRELNLSSAGITIL
                     WGSFLIRYEMNHHSFLGNTHTFGLVFSQVGFSYISADKPVKKEKPKLDIQSCTAEELI
                     ELNVVSEVLCQRIIKYRKMFGNVSVTSLYQLGLTTQQIRDLQQYTYNYYEDETNGIQN
                     HEKQSSIKQNNYIPHEEKNRRIKILFQSMVAAEIPAYIAISLAEEYQKSGEKGVLHSP
                     LFKNLTPSQQKSVKTTCGIQ"
     gene            complement(26973..27941)
                     /locus_tag="PLV81_07260"
     CDS             complement(26973..27941)
                     /locus_tag="PLV81_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09934.1"
                     /translation="MRWILLVYLLTPSLVWGQLLINEIACNVPGSDWVELFLSSAQPL
                     SMDISSLYVTMYYGTNEPLSQNPVTIYSYDRAETPWDDRFVVVHLTDATTPDECDATG
                     DINGNGVLDVYCNNYVNSLWNTDCVVAIDTDDDPSNGGIIDFVAYSNRDGTLNQSIVQ
                     YVGAAIQHGQWLGTASEEYMVDIGKNGLQEYQSIIRKAASDSNSSDDFVITPYLTPGH
                     PNILPVNHSNDTFIKINKKVVLTKRAIEQCKIFILQPCMLRVRLFSSQGYKMYESELL
                     SALPGFYTIDWVRQIKKMRCGLYLGTVEAQDSKNRQSLKFYCIVVE"
     gene            28115..28747
                     /locus_tag="PLV81_07265"
     CDS             28115..28747
                     /locus_tag="PLV81_07265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOM09935.1"
                     /translation="MVKLDNPYAKQRPSWKVFVPMLLVYPILKWVIHYSYFSFLYQYS
                     IALLILIIIAQYLDERLFTKDRIFSFDFPFERKIFYLFVMIIVLIPIRNIAVQFTPTP
                     QLLNFYQGKIKTLESVLIMVAIIAPCEELFFRGFAQYNLSFFFGKYGGVVIAALLYSI
                     TGIAAHSLYLAVLFFIIGIVIGLIYSRVQSLVQATLLHIITIIFMYLFPF"
     gene            complement(28744..28890)
                     /locus_tag="PLV81_07270"
     CDS             complement(28744..28890)
                     /locus_tag="PLV81_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09936.1"
                     /translation="MYSVFVFIMVIASLLVLFILPRIESKPDKCDISNNWQSVWEERL
                     KIDN"
     gene            complement(29016..29768)
                     /locus_tag="PLV81_07275"
     CDS             complement(29016..29768)
                     /locus_tag="PLV81_07275"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOM09937.1"
                     /translation="MRLIENLEAKLDDLTHKYGLVALKGGTEVEDMTYDELLFLKQIA
                     INLPVIVKIGGPEARNDIRYCKSIAIDGILAPMIESEYALENFITAVLDIYKEATLPY
                     LAINVETITAYHNLSAIYTNPYFSYINQITVGRSDLSQSMKKRVDDDDVLYATANIVK
                     AAKLSGKITSVGGQVNPSNAEVVQKVIQPDRINTRHLVFDCKKADNISKSIQLGLEFE
                     LDLYRAFSNVEPSKSHIYQKRISVTSDRLAVV"
     gene            30137..30340
                     /locus_tag="PLV81_07280"
     CDS             30137..30340
                     /locus_tag="PLV81_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOM09938.1"
                     /translation="MAKGTVKWFNESKGYGFLSQDDGKDVFVHHSGIAGDGFKTLFEG
                     DRVEFSIEQSEKGPRAVSVRTIR"
     gene            complement(30405..30824)
                     /locus_tag="PLV81_07285"
     CDS             complement(30405..30824)
                     /locus_tag="PLV81_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HOM09939.1"
                     /translation="MQIVYTLLDGFFLVFHSAFTIFNMFGWISVKIRKVHFFTMVATG
                     FSWFFLGIWYGWGYCFCTDWHWQIREMLGKPVPFNSYIQFLIYEITGYIPDANITDFF
                     VVTVYFMSLLISIVLNIRDYHNAKKYTIHDEIKKPDC"
     gene            30987..>31176
                     /locus_tag="PLV81_07290"
     CDS             30987..>31176
                     /locus_tag="PLV81_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOM09940.1"
                     /translation="MLLSGTIVIDITQFISGSRCTQILADMGAEVIKVEHPKGDTLRL
                     IFQLMAGAERCYSVFNRNK"
BASE COUNT         9792 a         6082 c         5258 g        10044 t
ORIGIN      
        1 attccaaagt tcaatacatc cttggaagcc tgaacagcta atggtgaatt ttcggctatt
       61 tccattgcca tcttttcagc gccttcatat aaagctttct gatcaggata cacttcatta
      121 acaagcagta tctctttagc acgctgggca gtgatgtatt ttgcagtata ggccagttca
      181 cgggtaatac cctgccccac aatatgcggc aggcgctgca atacaccaac gtccgcaaca
      241 aatccaacgg cagcctcacg caggctgaac tgggcatcag cagttgccag gcgaatatca
      301 catgcagaaa tcatatccaa cccggcacca acgcaatagc catgaacggc agctataaca
      361 ggtttacgac atttttcaat gcatgtcatg gtatcctgca ttttatagat cttctgtaat
      421 aagctccgtt tgatgccgcc cttttgtgca ggatcggcaa gttctttaat ttcgccagcc
      481 atttcaatta aatcaatacc agcggtaaat gcaggaccct taccagcaag tataattacc
      541 ctgatttcat catcagcgtc caattcttta aacactgaag gagcctccca ccatgcaggt
      601 ggattcatag catttttctt ttcaggtctg tttaaataca cccaggcaat tggcgccttt
      661 ttttcaatta aataatattt ataatccata ccctctacct cttttaagat atttaagtac
      721 acagtattgt atactcaatt ttttatcaat caaaaattac agtgttatgt tttatatgtg
      781 cgatagttac tgttaaaaaa ataattatgg tttataataa ttttttattt cacatttttt
      841 acaaagtatt gtatatatat aatatattct gattgtttat gtgttctcag catgattaat
      901 tttttaccaa ccatcacgca gggaacatgt tagtacgtaa aggacaattt ttatagtttt
      961 aaaaaaacat gtttcacttt ttgggtcatc attgatgacc aataagaata ttttttccca
     1021 gggaggttat atgttaaaaa aactgttcta gcattttgta tttcagtgtt tctcagcatt
     1081 tttctattac cgttttcatc acaggctcag gttaacagtg gtgattttgg attaatccta
     1141 aatggtggcg ttggtacagg ttcttcaatg tacggcgtta tactcaatac cacaaacgat
     1201 tcaagttcaa cattaggggt aggtcctggc agcagtttta atgctggttt tatggcaaat
     1261 tttaacatca ttgcaatcca ttcacaattg tactatgtaa cactaaaaga tttagaatgg
     1321 gaacaggaag tttcaggagt agatcgtaaa ttcaagactg aaggttctgg ccatttctgg
     1381 acatgggatg gcaccatagg aattaaagca ttaaccgaag aaggcgatat gggatataca
     1441 cacgtttatg tggggttccg tatatggaaa gtactccggg aagaagataa gaaaactgta
     1501 gatggtgtat ccaatccttt atatccaccg gtcaagcagg aattaactgg taatggattt
     1561 atagttggta tccgtgattt ttcgacatta ccgttaggat ctatctcact tgcattgcaa
     1621 acaggtttat ggctttacaa agccccttta agtaccttca agcaagatgg tactgaaata
     1681 gacacaacag ccgatcagaa tattggcttt ggatttgaaa ttggcggtgg gctcgcattt
     1741 gaggatattg ggctttcgac catagttggg ttacgtatgg atgtacaggc cactgagatt
     1801 aaacttccat taaatataga tgcagtagca ggttcaggat atgcacaatt cttctttgct
     1861 gttagcaagg aatttggatt ctaataaata aaaaaacaca ggcagggatt aacctgcctg
     1921 tgtttataaa caatatggta cttacattat attccagttg agcgggtgcg ggtaccaaca
     1981 ccaacataga aagccatatc atagtttttg tcaattttcg tgaaaatatc ttcaaactgg
     2041 ttttgcgtca gattccagcc aaagcgtgcc tgaaggtcta aataaatacc ttctgcaatg
     2101 ggtgtcataa agcctgctac accatttacc cacaggtcaa cttcactatc aggttcatct
     2161 tgaccctttg gtaaatagga atccatcaaa tacctggctg aaacacccgg gccaatataa
     2221 aaaccaccag gagctaagaa cttgagtgtt accatggggc ttaaaaagcc ataatcgtat
     2281 gctttatcat ctttatatga caaatatgca tattctaaag aaaatccaag aagcatacca
     2341 cctaaattaa ggttatattc accttctatg gcaacaccat taagtttaat atcctctgca
     2401 ttagctacat ctggcttcaa tgtattccac tgatacccta cacgcaaaaa cgctacatca
     2461 tcagtcatct cctgtgcctg tagccctgtt gtcacacatg caacagcagc aacaagaata
     2521 cacaacgcta acttcttaaa catcgtaaaa cctccttact tttgattttt tattatgttg
     2581 cttttttgat acacaaacta caaatgcact ttattgttta tatatatact ggcaacaaaa
     2641 attgcaagct ttatttttta tccaaagtta ttgaaaaaaa tttatcattt gtctattcct
     2701 aattgctttc gtataagttt acatagacta tcttttattt ctgcatgacg tggtactggg
     2761 gcttttttcc cattagaagg attaacatat atatcatgat tggctccatg tctttttaaa
     2821 aaacaacctt cctttattaa ctcctgaata aattcttttc ttttgaaact tcaacctcaa
     2881 tttccttcct cttggtttca atatatactg gttcaacttc ctccaccatt agtttatatg
     2941 cttcacgtat attgactatt aattcttcaa gtgtttcccc ctgactaaat actcccggta
     3001 cctcaagcaa tcttccaaca taccatcctt catctatcca gtactcaagt gtgaatgttt
     3061 tggtcatata ctttttatcc tcgtaatata ataagtgttt aatactgatg gattcagaaa
     3121 taaagataaa attaatttca ctaaatgtaa agcaaaaata acgatattta cgcaattgcg
     3181 tcaaaaaagg tgccggggat gactgctgac atgcttttct ggattcttgc tgtgttgctg
     3241 tacagtggtt cggggttttc ctgtacctgc gatagcaacc gtgcatgctc atcaggagtg
     3301 ggctttatgt attgaatgtt attctctaac ctgcgtgcaa cgccttttac cggtgtgatg
     3361 cgtgaatcat catataccgg ccacaccatc accgtgcgag caggttttgc tgtagtttgg
     3421 ggcagtgtaa taattgataa accatcagag attctcatgt agccattatc ggcataaatt
     3481 atgaaaatat tgagttaata acacggggcg ccatgtaatt atttatgaac aacataccca
     3541 atttcggtac ctgccttccg ttatctcttt taatcctttc cgcaaatcct ttaccagatg
     3601 cacatcacca tagtatttct tccatttcct gatcagatac tgttatttca tttgtgatat
     3661 atgataatgt tgcatattca ctaaaatctg acagagacct tcgttcacca tctgctactt
     3721 ttttttattt catatacagc accatcaact cgtagaggaa tagtctttgg cctactttcc
     3781 tcgtattact ctattttata acaccataat ctcttcctgc attccaccat atatagccac
     3841 ttacacctga atctgtgcag gcctctatct gcctctgtat gtacgtatag ccccatcctg
     3901 gtgaaagcat attgaacgcc tgcaggtacg gccgcaccac agcatgtgat ttcattaaag
     3961 ccctgcgatt gctttcgata actatcgcat aataatcccc aggatctatg tttccttcaa
     4021 aaaatttcct gccgtaatgc gaaggatata ccatggggca caatacatgc actattgatg
     4081 acatagcttc aatatcctgt cccaaccact gaccaaattc ataccatacg gtaaagccat
     4141 acacatccac acttattggc acatgaagcg cctgtctggc agatcgtaaa aattgcgcta
     4201 tagcctcata cttataaatc ccattggggg gtttatacgt aaatacacag cgatgcactg
     4261 gcccatcggt agggaagcga atataatcaa attgaatctc atcaaagcct gccctttgtg
     4321 cttctttagc aattgcaata ttgtagctgt gcacaaactc tgcatacggg tcaacccagt
     4381 attcttttgg attgccgcgc catgctgctt ttgtagcact atccttaatt gcatattttg
     4441 tatcaaaggc tttaaacagg cgctcatctt taaataccac catgcgggca attgctttca
     4501 tggtatggtt gtgtacatac gtgcacacct cttctggtgg caggccctta cgcaatgctc
     4561 caatagattc cgcagttggt tcactcacat acacattgcc ataatcatct ttaatatcaa
     4621 ataccagagc attgtaacca gccttctttg caacagcaac aagctcttta actactttcc
     4681 atgatgaagg cactcctgca tacagtccgc gtacagtgtc tttttgttct tcaattgcct
     4741 ttacaaatac atactgtgtt ccttccaaaa acaatacaca gtgagcctgt atgggcgata
     4801 gttcctggtg aatttccttt tggccggtaa gtgtaaccac agtgtcgtta tcaaccacaa
     4861 ccactttgcc attaatactt ccaccccaga atgtacctgt atgtccgctt aatcgttttt
     4921 tccactcttt tccgttatac gaatacactt cgtttgtata cgttgtgatg caaaactccg
     4981 gtgaaactcg gtacacttct tcaataaaca caatgctgtc agaatagtgt tttcgtggaa
     5041 ggccattgct aatattgata aaccttgtgc ctgtgtatcg gtacacacca ttccatgatg
     5101 tccccacaat gagagtgcta cccttagcat agagtgccgt tatgcatgca ttggctggaa
     5161 ggccagtaag ggcaacactc tgccagcttg taccaccatc ctctgagcaa tacagtgaat
     5221 acttggttgc aacgtaaaga atactggggt cagagccatc aacacagaac gctgatatac
     5281 tgcggtaggt gggaagcaac ccgtttgttg tccgtatgtg agcatcacgg ggggtgatat
     5341 gttgccagga actatcgccc aggatacgtt taaataagcc gtgctcacgt gtggaaatat
     5401 agaatgtatt ttttataacc tgaatgtctt ttatatgaat tgtatcaggt agcccctgat
     5461 gatatggctt ccccgtataa tataatttcc cgtttgtggc ctgatacaga agcggctgcc
     5521 atgcatagga aagtgatgta actaataaac aaacactaag tgctaaactt ttcattgaac
     5581 atgctttcat tcatgattat ataggtatag ccctgctccg ttaagaaaag ctggcggtta
     5641 tgcgcaaaat tttcctcaac tgtgtgcgcc gtaatgagcg aataaaaatg cgctgtgtta
     5701 tcccccggct ttggacgcag gatgcgcccc aaccgctgtg cttcctcctg ccgtgaacca
     5761 aacgtacccg atacctgaat ggcaacattg gcatcaggca gatcaataga aaaatttgca
     5821 acttttgaaa caataagccg tgttatagca ccttgtttaa attgtttata cagctcctca
     5881 cgttctttta cgggagtgct tccggttata agtggaaatg aaaaacgctc ctgaatcttt
     5941 tgcagctggt caatatattg cccaataata agaacgcggt cattgggatg atgttttaac
     6001 aattgttcca catagtgaag ctttttttca ttttctgatg caatcctgaa cttttgcctg
     6061 tcaccagcaa ccgtatactg atagcgcagg ctttcgggta attcaatgcg cacctcggtg
     6121 catattgcct ttgcaatcca gttacattcc tctaacaact tccagggcat atcaaatttt
     6181 tttggcccaa taaggctaaa cacatctgtt tcaagtccat cctctcgcac cagtgtggca
     6241 gtaagtccca aacggcgctt tgattgtatc tcagcggtca tacggaatac cggcgccggc
     6301 aaaaggtgca cttcatcata aatgattaag ccccagttat tctgagtaaa cagaccaaaa
     6361 tgtttgaatt catcttcttt tttgcgtcta taggtcaaaa tattataggt ggcgatagtt
     6421 actggtttaa tgtctttaac gtcaccagaa tactcggcaa tcatatcttt agttaatgta
     6481 gttttatcca gtagctcatc aatccactgg cgcagagcaa cagtgttggt tacacatatc
     6541 aatgtttttg tttgatacag gtgcataatg ccaataccaa cgatggtctt ccctgcacca
     6601 cagggcaaca ccaccacacc gcttccaccc tcatcacttc ctgagcgata gaaactatca
     6661 atagcactct tctgataatc gcgaattgta aactggatgc cgcttgccgt aacacttcgc
     6721 aactcaaacg ggcagggatc accatcaacg tatcctgctt tatcctcaac gggaaatcca
     6781 atttttatca gcgcttgttt tatatgaccg cgattgttag gctttacgag tatctcatat
     6841 tcagaaagtt gctcttcaat aaatttatga atctgtttat gggtagctat ctcccgtata
     6901 atatgcacat catcagattg caggataagg ttttcaccac gctttatcag ggtaagcaaa
     6961 ccatagcggt ccatctgctc ttttatggtg gtgatgacta ttgcagggac agtatagcgc
     7021 gagtatcgtt caatacgctc cacaatttct tttgcagtaa gacctgctga ggctgcattc
     7081 cacaatgata acggcgttat gcggtaggtg tggatatgct cagggctttt ttcaagctca
     7141 gcaaatggaa gtatagcatc ccgcacctct tcatacaggg gattatctac ctcaagaagt
     7201 aaagtattgt cgctctggac gatcagtggt ttggtcatgt gttatagtgt agtgtgtaat
     7261 attttttcaa gtaataataa ttcttttttg attctgcgct ttaatatagc tctaagaatc
     7321 ggtgtaacaa gaaaatatcg gaacggcaac ctgattgagc actcaactgt aaccgttgcg
     7381 gacacattat cacccataac ggtatatctg tattcgtact gcttcaaagc tcctttgagc
     7441 attttgagct cacagtattt atggggaact gcagtatatt tgagccttag tggaaattgt
     7501 ttaacaataa aacgtacatc aacgtcaata agctgtaatc cttcctcatc acgagctatg
     7561 cgatagctcc gtaaatcagt cttcaaccgt gccagcttat taatatgatc cagtgctgca
     7621 aaaacttcgg cagcagaagc gttaaagttt tgtgacagaa tgacttttat catacttttg
     7681 ctatttccct caaatgcaaa atgtattgtt gatggtatag gaatatttgt caaatattta
     7741 ataatcttat ttagagggag agagatagag atagaaggtg gttgtatgcc tgatgttatt
     7801 ttaatcacag tgaaaaaatc ctataagcac acctataaaa ttgatgttca ttggaacaat
     7861 ttttggtgaa ggtaataaac aaggggatac ggcgttgatg tttgaatatc cttatcccct
     7921 tatgaaaaat aacattataa aaacatcaca ctttttcttt tgttgccagt atttccagaa
     7981 ccttcttttc atcatatttc aagaaaagaa ttaatccagc aatacagaag aaaaatgcca
     8041 caacacacgt taatcgtaca cctatatcat tctgtggtgt tttgcccagt aatatgagtg
     8101 acgggaatat aagtgcagca atcatctgcc ccattttgga catgaaggta cgtgctccaa
     8161 agaaaattcc agctttaaaa ttacccgtct gtattccatc agcttcagca atatcagcaa
     8221 tgatggcatt gggtaaaatt ccaaaaattg ctaatggtaa agacgtcata acaataatga
     8281 ttaagccctg tacattttta tcgattggga accatcccaa catagatgcc acaacgtaca
     8341 ccgatgaata cagtacaaat gcaaatacca gtagcttttt cttcccaatc ttctgtgcta
     8401 tcaggtttac cgggacatag aatataaaac ttaatgcaaa taaaataatc atatactgag
     8461 aatatacagc tttatccagc tcaagtaata ccgttacata gtacacaaga ccagttgtaa
     8521 tgaatgtaag tgcaacccag taagccaaat ctgagatagt aaaaaataag aaatttttat
     8581 ttttaaaggc tgaacgtact gcctcaaagg taccctcatt ggaaacattg ggctcacagt
     8641 atcgccgttc atcaatgaaa ataaccggta aatacataag taccaatgat acgccactga
     8701 aaattatcat tacaacctga aatgcttttg tggaactgta accatagctt ttttcaaaca
     8761 atgactgtaa tgcaactgcc tgtgagccaa ttgcaaatcc tatagcccag gtaatagaaa
     8821 tcaatgtact caaaaataac cgttcattgg gattgtgacc caattcagaa agccaggcaa
     8881 aaaaaggtgt tacatacata gtcataaacc agtaaaacag tgttacaacc acaaaaagcc
     8941 atacagcatt ccatgttgaa agagtatcca caggtggcat aaacgctaac attcccagaa
     9001 ttgcaaaggg aaatgcgcca atggctaaaa attttctcct tctcccaaac ggtgaacgtg
     9061 acctatcgga cattgttgct ataatcggat cagttatagc atcccatagc cttccaaaag
     9121 catagataag cccaataatt gttaacactc caatcacata gccttgatag atatatgctg
     9181 ggaatatggc tttatcagct gactcaggtg gtagataaaa atatattaat aaattaccaa
     9241 cactgaatga agctaatgac caccccaact gccctaaagc atagatgatc tttttccaca
     9301 acggtaaggt ttcaacgttg tattccatac tgttttctcc ttgcaggtaa tatcatcctt
     9361 ttattcattc ataaggaaga aagataatat atcgataacg atattattaa accttaaatt
     9421 aaaatcaatc cttttttatt gaatacatat aattatttta tggcattttg taaatattgt
     9481 acagaataaa attttcaaat gtcttgggga tttactgtca tattaatatc cagaatcaag
     9541 cctttataac cgaaagccgg gattcagaat aactttttaa aaaaatattt tattagttta
     9601 tggtataacc atcactgcat gcgtacccac cttttgaatg aaattgttgc aatataaaaa
     9661 aacccccacc ctttatattt ttaaggcggg tttattctat ttttcagtta aaattatttt
     9721 accatgacgt tggtgatgca actttttgcg tttgcaaaaa gattcgcgca tcaacagcca
     9781 tgcacccatg cgaatacacc ataactggat tgcattcaat atctgaaatt tcctcaattg
     9841 aagctatagc atgacttaat gtaacaatga catcactgaa tgcattggcg tcaagtggct
     9901 ccttacctct gaacccttca aataaccgtg ctgcttttaa ctcccgtatc atttccaatg
     9961 cttctgcctt tgtaacagga gcaagtcgta tagatacatc cttgaatagc tcaatataaa
    10021 tacctcccaa tccagcaaga actattggtc caaaaaccgg atccttttta gctccaacaa
    10081 aaaattctgt tccacctggt gccatggtgt gcacatacac gccagaaaac acagcattgg
    10141 gattataatc atataaagac tttctcatgt tatcaaaaac atctttgagc tcttctttag
    10201 tattaatgtt aagtttaacg ccttcaactt cggttttatg tattgcatcc ggcgatacta
    10261 tcttggcagc aactggaaat tgaaattctg ttttttcaat tttttctgct gattgaacca
    10321 atattccatt aactatcggg acccctaccc gctgacaaaa ttgaattgat tcatgtaaaa
    10381 gaggttctct gttttccttt cctattgtat caaaatatgg gatctgagtt ttaaccgaat
    10441 tcatcatggt agtatcaata atacccctgt tactgcgcat tgccttcttt tgatagtaat
    10501 cagcagaaat ggcaagtgcg tgtgcagcat tgtacggtgt attaaaaatt ggcattgggt
    10561 ggttcatatt aatatcaagc atttctttag catcagaaat cattgccacc atgaccggtt
    10621 tgttatattc acgcaccaga cgttccactg tatcaaggaa tgtgcgtgac atgccagctt
    10681 catattcaga ttggtacaga tggttaaata agataccatc aacctcatct aatttgattg
    10741 tctcttcaac aatcttacca aatattgtgt aatcaaatat ctcacctaaa tccagtgggt
    10801 tttgatgtga aatgacccgt gttttatata ggttacggat agtattaata tagctttcag
    10861 gaaacgtaat aagattgaag ttatacttgg cacaagcatc agcagaaata acagcatgac
    10921 cgcctgaacg tgacaagacc gcaacattcc ttcctttcat tggtggaagt tgcattgctt
    10981 tcacagccag aataacttct tcttcatctt cagcacgaat aacgcctgcc tgcgcaaatg
    11041 cagcatcaac cacagcatcg ctagctgaca gtgctgttgt atgtgattgg gcaatcttag
    11101 cacttagtgg agacctgtta gatttgtgta caataattgg tttttctgat gcacgggcta
    11161 tatcaaaaaa ttccctccct ctattaaatc cttccagata gcatataata attcttgtgt
    11221 tatcatcatg attgacaaag taatctaaga aatctacctc atcaagcatt agcttattgc
    11281 caatacttac aaatttattg ataaatatat gattttcagg tagtgcatgc agataggtat
    11341 tgccaacacc accactctgc gaaatcattg aaacttcgcc tgcaactaaa tccttacgga
    11401 aaaatgcaaa cggcatgaac atcttttttt caaagttgat agtacctata caattaggac
    11461 caataatttt tattccgtat ttgttcgcta tcgcaagaat ctgttgctcc aatgattgct
    11521 ctgattgtga atattcactg aagccacccg attctatgac cacatgctgt attccctttt
    11581 taccacactt ttctagctgt tcaggaacag tttttgcagg tgtaatgatg atggcaacat
    11641 ctggcacttc aggcaaatcc aggatatctt tatatattgg aacaccttca atccggcctt
    11701 cctgtgtact tacaccaaat agcttccctg tatacccctg ctggatattg ttaagaagta
    11761 taatcttccc taaattaatc tttgtcagag aaactccaac aactaccata ctttctggat
    11821 aaaaaaatgt tttcatgatc ccctcatatc tttataatac aataatacat atataacaat
    11881 atgttataga catattttta tagtcaagat ttttaataat aattatttta tttggtaaat
    11941 aaagcaggtt gatatgtttt ttcaccacac cttaaaacac accataaaca actcccatta
    12001 tgggaataca aataaatgct cattgcttta tttataatcc agaaacagca ggatggattg
    12061 taattattga gcagtatgta cagactttat gataacactg ttatcaacat atcaagatga
    12121 tccattaatt ccctggtaat aaggaaattt tttcttacat gttctttacc attctttccc
    12181 atttcaatcc tcatctcctc attctttaaa agttctacca ctcgttttgc acaaccatct
    12241 atatcatacg gatccaccaa ataacctgtt ttcccatcta ttacctgtaa tgggataccc
    12301 ccaacatccg acgctaccac aggtgtgcct ttccataaag cttcagaaac tgtaagtgca
    12361 aagccctctc gtaaagattt ttgtaatact actgcagctt gagtctgcaa aatattaacc
    12421 agtacattgt tttcttgagt atacaggata atatcttttg aattcttgat tttattaaaa
    12481 attttattat aaataaccat cccttcaggg tcatcacttg ccattgagcc gcaaagaata
    12541 agtctgcaat ccacttcctg ctttaccttt tcaaaaactt ttataacacc ctcaggatcc
    12601 ttccacttat caaatctaga aatttgaaca ataaaaggct tatctattgg aatattaaat
    12661 tttgaaatat atttttgcac tactgccttt ggtaattgca tattctttgg cgttaatgga
    12721 tctattgatg gatgaatgac gtgttgcgga atagggatat cggccttttt atatttttca
    12781 tgagaaatga ccatcatatc ataatgtaaa ataaatgttt tgagatattc ccatagtttt
    12841 atattttgat ttgttatatc tatatgcaat ctccagatcc atggttggcg ttttctgtaa
    12901 tatcgtatta ttggcaatgg ttgtggatca tgcacaatta cagcatcatg attgatatgg
    12961 gtaaattttg caaaattata atttgtttcc tcatataact gacatttcat ttttgtaaaa
    13021 tggattgaat caccctgtaa tgcattatgg aatttttttg ttattgcaaa aaaatcggga
    13081 gtgccatgca atatacgcca ccctgtaaat acaccggcat cattcattaa tagcaccagg
    13141 ctattcaata tctcagcaac tccaccacct tgatatgttg aattaatatt tacaatatga
    13201 tatttggata atttattagc tttgctatat aacatactta taattgaatc atcaattatg
    13261 cctctataat catcaatact tttattcata attttgctcc ttaagttttg ataaaaattt
    13321 tcttacggat tggtaatccc ttatattgaa acgcgcatag gattgaataa ttccaacacg
    13381 tatggtatat gcttcttctg tcaatgcctt gaatagatat tcgtcggtcc aatcatcgcc
    13441 aatagccaga atgaaatcgt aatcattctt tgaaattata cttaacgccg ccgttccttt
    13501 atccattcct gaattcttta tttcaataac tttattaccc tgcataatct gtaggttcat
    13561 gtttgctgca aaacttataa ggtaatcggt taattctttt gctaataaag aactcatttc
    13621 agggtcagat ttgcgataat gccataccaa tgaatactct ttttcctcaa taaatgattg
    13681 aggtaagcgg tccacatata tttcaagaat ttgatttata gttggtttcc aatcatcagc
    13741 aggagtaata ttaagcttcc aatccaaacc attctctttt atccatatac catgttctgc
    13801 aattaaaata acatttaatg ttccaaacca tttttctaat gtatctttat ctcttccgct
    13861 gataatgaca acatctgtat tattcatgtt ggacagcaat ttcagttgat ttaatatatc
    13921 ctcatctggc tttgccaatt caggttgttt ttcaaaaggt accagtgtcc catcgtaatc
    13981 taaaaataac actttctttt tagcattctt gaaagcatcc actatctctt tttccttttc
    14041 agaattgagt aacaatgaat taaatttttc ctgctcagct ttcactaata acatttcttt
    14101 tataaagttt gttgcccatt tgacaacatt atatctttcc aggcgttttt gcataatgac
    14161 atttctttta atctgctcat cttctgtcat aattaatgct tcttcaatag cacatactat
    14221 atcattaata tcattaggat ttacggtaat agtttcaccc agctcacttg cagcccctgc
    14281 catttcactc aaaataagca cacctgtttt atcaaagcgg gaagctatat attcttttgc
    14341 aataagattc atgccatccc tcagtggtgt aataagcgct atgtcactta tagtgtataa
    14401 tgccaccagg cgatagaaag gttctgatct gtaaagatat attactggtg tccatttgac
    14461 agttccatat tcaccattta ttttacccac catgcggtct atctgtcttt taatttcatt
    14521 ataatgttct acttcagttc ttgatgggac aacaagtagt aacaatacca ctttttcgtg
    14581 ccactcagta tatctttgta aaaacttttc aaacgcatat aagcgattta atatgccctt
    14641 tgtataatcc agacggtcta ccgatagtat aattttaacg tcaggaagtt ttttcatata
    14701 tgattctcgt gccttcctta cttcgggtag tttaattgca ttgttaaatt tatgaaaatc
    14761 gatacccatt gggaatgcat ccacttttac aatatggtct tccattatta ttttattcat
    14821 gtcattgctg tatccaagta tatataatgc acatcgtaaa aaatattgtg tgtagctata
    14881 cgtatgaaat cccacgcagt cagcgcctaa aatacccata agtatttcct gagcccattt
    14941 ttttggtaat aatctaaata tttcataatc agggaaaggg atatgaagga aaaatccaat
    15001 agcattattt atagattttc ttagcatgcc tggtaataaa agcaattgat aatcatgaac
    15061 ccagactata tcatctggtc tcagtatact cataactgcc ttataaaaca tattattggt
    15121 atatttatac gcatcccaat aatcatcatt atatatagta tattcagcaa aataatgaaa
    15181 taatggccat attgttttat tgcaaaatcc atagtaaaac ttatcaataa gttcttcttc
    15241 aatatatacc gggtagcaat tataagaatc aaccagagtc tttgttattt cgcttgtctt
    15301 tactaaatgt tcaagattat ccattggacc cggccagcca atccatatat attcaacttt
    15361 ttctgacatt gctttttgca gagaatcaac atatgatttt attcctgttg ccagtccacc
    15421 aacactttcc ttaaaagtga tggttccacc actttctgat attgtaactg gcaatctatt
    15481 tgaaacaata acaagtctca ttatttgctc ccattaaaaa tatttctact gatatccata
    15541 ttctatgata tactatatta ctaatttata tctttcgcaa tatataccat actggttgga
    15601 tatgcaaatt cgatttcatg ttcctcaaat gcttctttta ttttaaaatt aatctcctgc
    15661 tgaatatcca tatataaatt gtaatcatta gattctacat aatatactat ttcatactgt
    15721 aagctgaaat ctccataaga aaagaaatga gctcgatcaa atgttgcatt atctatattg
    15781 cttacaattt cctttattag ccctggtatc atctttaatt gttcaatttt ggtttcatat
    15841 gttacaccaa tcctaaacac tacccttctt ctatgcatcc ttttataatt acgaacccgt
    15901 gcattaatga ggtttgaatt tgatataatt atctgctcgc cacctaaact ccttatttta
    15961 gaagatttga taccaatatt ttcaactgtc cccattaaat ccgtatcaac aataataaaa
    16021 tcatcaatct caaatggccg atcaaaaagc ataacaaagt aagcaaacaa atcacctaac
    16081 acattttgtg cggccaaccc aattgccagt ccaccaatac ccaaccctgc aattaagggg
    16141 gttaatttaa ttcctaaatt ttccagtaaa aaataagaag caaaaatcca tataatgacc
    16201 tttaataaag gattcaatgc ccgcaaagct ctctgtttag tagtatcaac ctggcgttgt
    16261 ataataacag aaataaaaat gccaacaccc ttcacaacaa gtgatacacc agatatagta
    16321 aagaatacca aaaaacatat atttatagcc tttcttacaa cgggttcaat cgacagttgt
    16381 aaaatgctta catataaaga aatgataaag gttaccggaa ggaaactatt ttcaaatgca
    16441 cgtataatga tatcatcaaa taccgtgttt gttctttgta ctattgattt cagctttctt
    16501 ataataattc ttttaaagat aaatatacaa cagagtgcaa aaataaaaat taatacactg
    16561 atacagtaat ctcttactga attcccaaaa aatgtttttg ctaatactga ttcaatcatc
    16621 tttaatcttt cccatcctta tataacatct tttcatataa acatatactc atcataacca
    16681 aaaatcatac ttcgatatcc tctggccata aaacgatatc aatataataa tttttatgcg
    16741 gttcattgca ttaaatagca taataatcat tatttacata taatgtattt tattcattta
    16801 ttataactat ataatattat atatatgtgc aatattgttc agtcaaatac ttttttaaaa
    16861 aaattatatt tttatcaaat atacagtatt ttagttgact tattttgcta ttcattatta
    16921 atgaacatat aaacaatata ttcatttatt tattattgct atgagaaaga aagaaaacac
    16981 tgaaaagata ttttctgcag cactgaaggt tttttctaaa tatggttata aaaaaaccac
    17041 cgttgaggat atagctaaag aactcaatat gaccaaaggc aatctgtatc tgtatgttaa
    17101 agataaaaaa gacctgtacc ataaggttgt tgagtttgca cttttgcgat ggcaaaatta
    17161 cgttcgccag gcaatacaca atgagtcaga tccccgcaac aagtttctta ccatgtgcca
    17221 caaggcagtt gagtatttat ccatagatgc tgaatttcgc aatatcctgg tacacgaccc
    17281 tgagatattc cccatgttta ctaataaaga cccctatgaa cgtattaata ataattcagt
    17341 taccatgata aaaaatatct taaaagaagg aattgggaaa ggcctcttta ggcctgtgga
    17401 tatatcagca atgtcaaagg caattttttc tatttataag atgtttgtta tacgcatgta
    17461 catacggagt gaagaaaaag atgtgcgttc aatgtttgag caaacactgg aacttattac
    17521 acagggtttg tttaaacaat aacttaatat aataattttt atttttagga ggtaacttat
    17581 gccccaggaa ttaatgtatt taacaaaaga atggcgagat gaagccgaac gacgactgaa
    17641 aaatgaacta acacctgaaa agatgaattt tatcacatca tccatgtcca atatttattt
    17701 aaattgcccg gatggtaaga acaaatatct ttttttcaaa tttattgatg gaaagcttga
    17761 tacactggct cttggtgagg gtgaaccacc tgatgctgag ttccgtatat ccggtgatta
    17821 ccaggttttt gccatgattt ccagagctga gttaggttca caaaaagccc tcatgacagg
    17881 caaattaaaa ttaaaaggga atatggtaaa agcattaaaa ttagcagcca tagcagacag
    17941 gttaaataaa gtattatcgc aaatacctgc acaatattaa tgtaccatcc taaattttaa
    18001 cattatgagg tatatactat gacatcacga ttagaaaata aagggccata ttatattgac
    18061 tttcctggca gaattaaggc tatccagcag gaaatggcaa aggaacacat tgatgtttat
    18121 ttaggttcac ggttacgaac attatcctgg actttagatg cgttttgccc atggaggagt
    18181 tttgttgtta ttcctaacga agggatgcca gctgcgttta cctttgttat tgatgccgca
    18241 cgtgttgctg atgaatcatg gcttgatgaa gaccatgtct ttggatttgt accaatgggt
    18301 ggacaggatc agatcgaact tattgctgag tatatcaaag acctgttaaa aaataagaag
    18361 ggaagaatcg gtgtagaaaa cgggatgtcc aattatctgc cagaaggcaa cctaacacac
    18421 tatgaatatg aacggtttaa agatgcactg cctgattgtg aatttgtaga tgcatacact
    18481 ataattgaca aactgtccat gataaaagat gaggggacca ttaacagatt ccgcgaagca
    18541 tcccgtattg ttgatgtggg tcataaagcc gtatatgaag ccataagcaa tggcggctgg
    18601 aaaggtatga ccgaaactga gattgctgga cttgcagcgt atgccatgcg caaagaaggt
    18661 agtgaatggg aatggagtta taccggtgga aatgaaatag catctggata cagaacagga
    18721 cttgctggtg gagcctgcac acctgcaacc cgcaaaaaat tgcaagcggg cgaacccctg
    18781 atggttgaca ttcatgcaat gttcatgtta ggtctggggg atcattcaca taactatctg
    18841 atagaacctg taactgaccg gcaacgctgg catgcacaaa attttcttga tattgtaaac
    18901 ttgacactta aaacatatcg tgctggaata acaccttcat tgttagccga gcagatgatg
    18961 gcatttgctg aagagcgtgg ctttgctgat tacatggtgc caggttttga acatgggata
    19021 gggatgatgg gtgatgaatg gcgtattgga ttaaatgatg gacctttccc attctggacc
    19081 aatcctgacc atgtatatca ggaaaatgaa ttaattatct gtgcaatgca gtatgcatgc
    19141 ccacaagata acattggatt caggtatgaa aatcctattg tcattcataa agatgggtgt
    19201 gaaccgttat caaaataccc actcactatt gacgaaattc aatagcctat ggcagttatt
    19261 accggttaca tatccattgc aacacagggc cagggtgata ttattgatat caccctggat
    19321 gcccagaaga tagttactag gaataaaatt caggatgggt tgctgtgcct ttttgtgcct
    19381 ggatcaacgg cagctatcac cacaattgag tttgagccgg gactacagaa agatataaat
    19441 ctatttttag aaaaaataat cccacgtaat gcacattatc atcatcatga cacctggcac
    19501 gatgataatg gttcttcaca cctgagagct tctatgattg ggccatctat tactgtacct
    19561 atagttgatg gaacactaac gttaggaacc tggcagcaga tagttgtcat agaatgcgat
    19621 acccgcaaga gaaatagaaa aatagtagca caaatagtgt attaacaaaa atgtcaacat
    19681 ttttgaaata ttgagtaact tacaaaaaga caatttttta taagtttctt catattcatt
    19741 tgacataatt attttttatc attataattt ttacaaatat atattaattt caatcatcaa
    19801 tacagaagga ggtccgtatg ccagaagagt ttaaacctgg atgtacacgt cccaccactt
    19861 cgccaccacc acaggacatc attttgcaag aacaacaaaa ttcatcaaaa aaggaggaac
    19921 acacaatgat tcaagttggg caaaaagcac cggaattttc tctgccagca tattttaaag
    19981 ggaaatttgt tgaagtaaaa ttatctgaat atctgggtaa atgggtagta ctctgttttt
    20041 acccgggaga tttcacattt gtctgagcta cagaagtttc tgcggtcgca gaaaaatatc
    20101 cagaatttca aaaattgggg gttgaagttt tatcagcaag cgttgatagc gtttttgtac
    20161 acaaaatgtg gaacgataat gaattgtcaa aaatggtaaa aggtggaata cctttcccta
    20221 tgctttctga tgcaggcggt aaagttggga caatgtatgg agtttacaac agcaatgctg
    20281 gcgttgaaaa ccgtgggcga tttttaatag atccggacgg agtcatagtt ggttatgaag
    20341 tgctgacacc tcctgttggt cgtaatgtcc ttgaaacaat acgtcaaata caggctttcc
    20401 agttagtaag agaaagtaaa ggtacccagg caaccccatc aggctggaaa ccaggcaagg
    20461 ataccttgaa gccagggcct gatctggttg gcaaagtctg gcaggtgtgg actgtcgata
    20521 aggcatttga ctaaaatagc gaaaaaatac ttgatttttt taaatattat attacaatat
    20581 tatatagtag tttgtttgtg ttaattaaac tatcaaacat ttgataatta tgagctatac
    20641 agcatattgc tttcacccgc attacctgca ccataaaacg ggagattcac atcctgaatc
    20701 tcccaaacgt ttagagatac ttgataaata tatccgtacc aataaaaatt attcctcatt
    20761 gctttacgtt cagccacgta aagctgatcc cgcagtttta acatatgccc atgatgccga
    20821 ttacgttaca atgatcgcca gtgcaaagcc taatggaata acctgtattg atggctatga
    20881 cactgtcata tgcgatgaaa cgtatgatgt atcgttatgg gcaattggag ggatacttga
    20941 ggtatgtgat tatatccttg ctggtgaagc caccaatgga ttctgtgcta taagaccccc
    21001 tggccatcat gcagagatag ctcaagcaat gggcttctgt atttttaata atgttgcaat
    21061 cgttgcacgc tatctccaat atacctataa tatacgaaaa atagccataa tagattggga
    21121 tgcccatcat ggcaatggca cgcagcatat ttttgaaacc gacccttctg ttttttatat
    21181 cagcacacat cagttcccct tttatcccgg tacgggcagt gttgatgaga taggcattgg
    21241 ccctggatac ggatatacta taaacatacc catgaatccc ggtagcaatg ataaaacata
    21301 caaaagtgct ttcactgaaa ttataattcc tgctttagac cactttaaac ctgaatttat
    21361 tcttgtatca gcaggttttg atgcccataa aagtgacccg ctttcatcat taaatctttc
    21421 aagcggggca tattacatgt ttactcgcat gcttatggat attgcaacac ggtattccaa
    21481 taacaggatt gtggtggtgc ttgaaggtgg atacgaaata caatcgatga tagaaagtgt
    21541 tgattatgtt cttaaagcat taatggagta attacctgaa aataaattca gcataattat
    21601 gatgttttgt agttctgctg gtaaaaataa ttcccagtgg ttttgaaatt tctaaaacat
    21661 gatcaaagac ttttgcagct atagtgccct ctaacacttc aggttgtata ttaatactac
    21721 catcattata tatccctttg attgcataaa tatgcatctt gataattttc ccatttcctg
    21781 acatatacag tgcacaggct atattatcat taattccttc atgaacagag ccaaaaagaa
    21841 aatttcccaa tgaataaaca attggtttat ttttatagat ttcaattccc tgatacacat
    21901 gaggatgatg tccaataaca gcatctgcac cagcatcaat gagtgcatga gctatatcaa
    21961 tctgttttga tgaaggataa taaaaatatt cattacccca atggatattt attatgacac
    22021 aatcacagaa acggtatttc ttaatatcct cctgcgcttt ctttaattca aaaaaattaa
    22081 ttccgggatt attttttgca atcatttctt ttgaggcgat ctgcgtatag cataacacaa
    22141 caatgcgtgt tgtccccaac tgacgcataa ctggcagcga tgctgttatt tcattgttcc
    22201 ctgctccagc atatagcact tttgcatggg atagagaagt aagtgtatcc agtaatcctg
    22261 taactccata atcaagcata tgattgtttg ccagggtaac tgctgtaata ccagcttcag
    22321 ccagaataaa tgcaattgaa ggtggtgctt taaaaatata tttttgatat actaaactct
    22381 ggctactccg tgtcaatggt gattctaaat ttgccataac aaaatcaaat gattggaaaa
    22441 agggtttaat tctttttaat gggtattctg taccatagcg ctgcattgtg tccttcactg
    22501 cccattcaaa catcatatcg ccaataaaca gcatgctata gtcagaagaa gttgctatat
    22561 agtgcttggg gattcgttcc tcaggtgttg tacatgatag ttcataaaca taaaataata
    22621 ctactatgaa aatagctgac tttttcacgt tttaatgccc ttttgtatat gggtaaaggt
    22681 gaagctcgcc tgttataaaa tctttaaggc cgggtagatt ttcaatatca tccagatgag
    22741 atagtaaatt tttctcagga tacatacatt caaattcttt tatccttaac ttgaaatgag
    22801 cagcctgtat cagtatttgc acaagtgcag catcagaaaa ttctgttatt tcaaataaat
    22861 cggcatgatt catggaaatg ccttttagcg gagaaaaagg gtctacgatg ttcaagcctg
    22921 cgtgctgatt gaaatactgt acaatatcgt tatattgctt tttttcatta aaagtttgtc
    22981 ctgtgaatct attcccggtg atgatatctg catcctggaa aaaagacagg taccccgaaa
    23041 ctgtatgcca atcagcaaaa gcttccaggg gcagcataaa caacgaactc aacgaataat
    23101 cctttgcgta tgcaaacgca ctcataagtg ccccaccata ccccagaggc tcttcatgca
    23161 taacaaaatg aacatccaca tcagaaacga ttgagggcga ctggtcaaaa gagccatcat
    23221 caataataag tggcatgaca tgggcatagg ggcaattgca gatagagtct aatgtacgac
    23281 gtaataattc ctcattattg cgaacaataa ttgctacaag aatatcactc atgatttaac
    23341 atcctgttta aattacctga tgtgaatcct tctaaatcaa tagtcacata taaaaatccc
    23401 aatttcttac aaaaagcaac gattttattt cgtatctcag ggtcaacgat tcttggcata
    23461 tcctgtctca ttacttcgat gcgtgccaca ggaaaatggt accgtacacg tacaccatca
    23521 aagcctaacg aaataataaa ttcctcagcc ctctgtatag cgtcaagtat atcctgagta
    23581 atgcctgtac cataaggaat gcgtgtggca aggcatgaat tggatgcctt tgaatatggg
    23641 atttttaaat gttgcaaacc ctgaataata tcttctttgg taaaacctgc ttcaagaagc
    23701 ggggaatgaa tacctaactc aataagtgcc tttcttcctg gcctgtaatc agaagtatcc
    23761 gacacattgc cagcatcaac cacattctga aaaccacttc ggtatgcata ttcctgtatt
    23821 tttttaaaca ctgctttttt gcaataataa caacggtctt tattattggt aactatctcc
    23881 acagttccca acactgcaag agtaatctcc tgatagggga tatcgtgttc tcttgcaaag
    23941 gtacgcccac gctgtgtatc gcctggtata gtaaattcgc ttatacagtg aataaggcaa
    24001 aaatccattg aaaaatgttt ggagatagct gctaaaaaag ttgaatcgtt gccaccagaa
    24061 taggctattg cataaggctt gagagcctgc aacttttttt gcaatatgtc tattttttga
    24121 tttgtcatgg gattacaata ccataatgtt actgaactat gtataacgat tgtagcacgg
    24181 tatcaaaaaa actaaaattt taaaaccatg ctgccgcacc aacttcccct gcagtttgtt
    24241 tttggggatg tagctgcata acacatcttg ctgacagctt acaatacgta ttactgaaac
    24301 gcaagcgtgc aacaacaaaa aatgatgatg cggcgatagt ttctgaaata atatttgttt
    24361 gtggaagcgg aagcatagct acatacattt tttcgccatc agtatgagca taaactgacc
    24421 ccaagtcatt ttgcagattg ccaactttta ttccaatacc tgatgcagta taccatgcga
    24481 ccgtatcccc ctgatataac ccatatttta gatggattga taagtttttt gttattcccc
    24541 atacaacccc ctgctggtaa cgcgatgccc tttcttcccc atctttataa aactgtgcat
    24601 agcgataatc cgatttaaca gaaaaatatt tgtttatata taccatacgt atattattaa
    24661 ttcgtttatg tggatattcg gtataatacg gagagggcag aaggttgttc tggtccatat
    24721 atatccatga acatgtaaat ttttttactg gtttaacact gaccgaataa taatacatat
    24781 ctcgtggtga atttccccca aatgtattgc caaaaggggc aaaaaagttt tcatccgact
    24841 tttgtacaat actgctgatt ttataatttt tttctttaac tattactcca caataatatg
    24901 ctgaactata tctgtttcct gatttacctt cattttttat caaaacatgt gaaatagcat
    24961 attccgaaaa aagtgataca atactgtcat tatagtgtgc aaacagactg tacccaccaa
    25021 atgattccat tgcatagtta tcctgtgtat ttgcattgaa taacatgtga gcattattat
    25081 aatatacatg tgttgaataa taacaaccct gtaacgttac atgctcaaca gggcttagcg
    25141 ttgcatgcac aaaagatgta acaacctgta caggttcagt atactgtccc tcccttttac
    25201 agtgtgatag taagccctgg acgctatagg ggtatacccc attttgtgca tcaccagaag
    25261 atatataaca ttctttatag gaaactcctg catcaagttt aatggtagat gcacctcttg
    25321 cgtcaaataa tgatgaaacc acaccataca tagcgtactg tggattgcca cttttacata
    25381 atgatacaaa tgaatcattt ttttgaacat gaatagaata gggatcaggg ttataaacag
    25441 aagtttttcc caataacaaa cctgtggcat tgtgaataaa ataatagcca cctgcaattg
    25501 cagcatagtc accctgagtt gcaaatgacc atgtagcaag cgttgcatca tgtgattgta
    25561 caactgctgc atttacttct gtagtatctg ttgctattgt tgcgctggtg cggaggtaat
    25621 gttctttttt aacgccataa tcaagagcat cctgcctgta ttcaactgat atactctgta
    25681 aagctattgc ttcaagctgc aatagtatag aaatatatat aagcaaatat agtattactg
    25741 tattccgcat gttgttttaa cactcttttg ctgagaagga gttaaatttt taaacaaagg
    25801 tgaatgcaat acgccttttt ctccagattt ctgatactct tcagctaacg atatggcgat
    25861 ataggcggga atttcagcag caaccatgga ttgaaaaagt atttttattc gtctattttt
    25921 ctcttcgtgt ggaatgtaat tattttgttt tattgaagat tgcttttcat ggttctgtat
    25981 tccgttagtt tcatcttcat agtaattgta tgtatattgc tgcaaatccc gaatctgctg
    26041 tgttgttaaa cccaactgat acaatgacgt aacactaaca ttgccaaaca tttttctgta
    26101 cttgataata cgctgacaca atacctccga tactacatta agctctatta attcttcggc
    26161 agtacatgat tgtatgtcta atttgggctt ttcttttttt acaggcttat ctgccgatat
    26221 atatgaaaag cctacttgtg aaaataccaa tccaaaagta tgggtattac ccaaaaagga
    26281 gtgatggttc atttcatacc gaattaaaaa agaaccccac agtatagtaa tccctgcaga
    26341 tgataggttt aattccctgc tatagccaat atcaactgca cagtaccgtg taatatagcc
    26401 attaatttta acgatctgtg agatataatc accataatga aattcatact caactgtaca
    26461 tcccttaaaa aattctgcac gtgctgatag tattgtttca gaagggatat atccatcatc
    26521 gtgaaaattt tttatattgt tctgcattac aaaaaggctt acccgttcaa aaggtttgat
    26581 ggtgcaatac atgccataat catataagct ttgtgtatgg gaaaacgttt ccgtatgaat
    26641 tgaaagaatg taatcatgga attccacgcc aacgcttatc cattgagcta tcgcccaaca
    26701 agcaccaaca ttaagcgtat cttcacgata ttcaggtatt ccaaagtact gccattgtaa
    26761 tcccactgaa taaccatgca tagcagtatg gataaatgta gttgttctgt ataagtccgg
    26821 atatccaaaa gggtttgcat atgttactcc agaaattaat ggtgaagggt ttgcctgata
    26881 cgctggtgct atcgcccata cattaagtgc cagtggttga gccgtttcaa acgactgtag
    26941 catcaacagt agtgaaacta gtagtgaagc catcactcaa ccacaatgca gtaaaatttt
    27001 aggctttgac ggtttttact atcctgtgct tcaacagttc ctaaatacaa gccgcaacgc
    27061 atttttttta tctgtcgtac ccaatcgata gtataaaacc ctggtaatgc tgaaagaagc
    27121 tcgctttcat acattttgta cccctgtgag gaaaatagcc gtacccgtag catgcatggc
    27181 tgaagtataa aaattttaca ttgttcaata gctcttttag taaggacaac ttttttgtta
    27241 attttgataa atgtatcatt agaatggtta acagggagta tattgggatg tccgggtgtc
    27301 agatatggtg ttataacaaa atcatctgaa gaattagaat ccgatgctgc ttttcgaata
    27361 atactttgat attcctgcag gccatttttt cctatatcaa ccatgtattc ttcagatgct
    27421 gttcccaacc attgcccatg ttgtattgct gcaccaacat actgtacaat agattgattg
    27481 agtgtcccat cccggtttga atatgcaaca aaatcgatga taccaccatt tgaggggtca
    27541 tcatccgtat caatagcaac aacacagtcg gtattccaca ggctgttaac ataattattg
    27601 cagtatacat caagtacacc attgccattt atatccccgg ttgcatcaca ttcatcggga
    27661 gttgtagcat ctgtcaggtg taccacaaca aaacgatcat cccatggtgt ttcagctcta
    27721 tcataactat agatggtaac tggattttgt gaaagcggtt catttgtacc ataatacatg
    27781 gtcacataca gtgaagaaat atccatagat aaaggttgtg ctgaagaaag aaaaagctca
    27841 acccaatcac ttcctggcac attacatgca atttcattaa ttaataattg cccccatact
    27901 aaagatgggg ttaaaagata tactaacagt atccagcgca ttgttggctc ctcatgttgc
    27961 tctttttgta taaatacgct ggaatgagaa aaaattaaaa gtttataaga aaatttttat
    28021 atttttttaa aaaatcttat gaaataatga aaaaaaatat aatacattga attaattgct
    28081 tgaccaatca tattttacag caaatatatt tgccatggtt aagcttgata acccatatgc
    28141 aaagcaaaga ccatcatgga aagtatttgt acccatgcta ttggtatatc caatattaaa
    28201 atgggttatc cattattcct atttttcttt tctctatcag tatagcattg cccttcttat
    28261 tttaatcatc atagcccagt atttagatga aagattattc actaaagatc gtattttttc
    28321 ttttgacttc ccttttgaaa gaaaaatatt ttatctattt gtcatgatta ttgtccttat
    28381 ccccattcgc aatatagctg tgcaatttac tccaacccct caactattga atttttatca
    28441 gggtaaaatt aaaacattag aatcagtgct gataatggta gcaattatag ccccctgtga
    28501 ggagcttttt ttcagaggtt ttgcccagta taacctttct tttttctttg gaaagtatgg
    28561 aggagtagtt attgccgccc tgttatacag tataacaggc atagcagctc attcattgta
    28621 tttagcagtg ttatttttta ttattgggat agtaataggt cttatctata gcagggtgca
    28681 atcactggtt caggctacat tgttacatat aataacaatt atttttatgt acctattccc
    28741 cttttaatta tcaatcttta aacgctcttc ccagactgac tgccagttat tgctgatatc
    28801 acatttatct ggtttacttt caattcttgg caaaataaac aatactaaaa gtgaagcaat
    28861 taccattata aaaacaaata ctgaatacat aaaaaacctc ataaaaccat tagcaattaa
    28921 agtaatatta attgaggaag gatatcgtaa gaaagagtgt ttttagtcaa gtttttttaa
    28981 aaatattttt ttaacctaat aatttttatg taatatcaca caacagccag tctgtcagat
    29041 gtaacagaaa tccttttctg gtatatatga ctcttagagg gttcaacatt agagaaagct
    29101 ctatataaat ccagttcaaa ttcaagcccg agttgtatgg attttgagat attatcagct
    29161 tttttgcaat caaaaacaag gtgacgagta tttattctat ctggttgaat aaccttttga
    29221 accacttcag cattggaagg atttacctga ccccctaccg atgttatttt accagataat
    29281 ttagcagctt taacaatatt ggcagtggca tataatacat catcatcatc tacacgcttt
    29341 ttcattgact gcgacaggtc acttcgtcca acagtaattt gattaatata tgaaaaatat
    29401 ggatttgtat atatagctga aaggttatgg tacgcagtaa ttgtttcaac attaattgcc
    29461 agatagggca gtgttgcctc tttatatata tctaaaacgg ctgtaataaa attttctaat
    29521 gcatattctg actcaatcat aggtgccaga ataccatcaa tagctatcga tttacaatat
    29581 ctgatgtcat ttcttgcttc aggtccacca atttttacaa ttacaggaag attaatagct
    29641 atctgtttta aaaagagcag ttcatcatat gtcatatctt caacttcagt gccacctttg
    29701 agtgcaacca atccatattt atgcgtgaga tcgtctaatt ttgcttctaa gttttcaatc
    29761 agtctcatag caattgtccc tttctatttt taatttactt tatactccta tacgattaat
    29821 cggcatttcc agaaaaatga taaaataaat taaggccgcc atcttacctt aggcggttgg
    29881 aaggtacact ttgtcatata ttattcacta aattatattc agtaataaca ttgaaaaaaa
    29941 cgtaattttt taagaaaaat ttattttatt tgacaattat tacgctatac ttactatgac
    30001 tagatagttg gtatcctctt aattacatcg tttttccttc cttatctatt ggcaagttaa
    30061 aacagtacat gtgggatgtt caacggtaac aggtataatc tcctgttata acaaattatt
    30121 tatggagcgt catacaatgg caaaaggtac agtaaaatgg ttcaatgaaa gcaaaggtta
    30181 tggctttcta tcacaggatg atgggaaaga tgtgtttgtt catcattcag ggattgctgg
    30241 cgacgggttt aaaactcttt tcgaaggtga tcgtgtagaa ttttctattg aacagagtga
    30301 aaaaggaccc cgggctgttt cagtaagaac cattcgataa atacaacatt tgtaagttaa
    30361 aagcttgtta tatactaaca cgttgtattt ggcaaaaaac ccgattaaca atcgggtttt
    30421 tttatttcat cgtgaatggt atatttttta gcattatgat aatcccgtat atttaatact
    30481 atagaaatca ataaactcat aaaataaact gttacaacaa aaaaatctgt aatattagca
    30541 tctggtatgt atcctgttat ttcataaatc aaaaactgaa tataagaatt aaaaggaact
    30601 ggtttcccta acatttctct tatctgccag tgccagtctg tacaaaagca atatccccac
    30661 ccataccata tacccaaaaa aaaccatgaa aatccagttg ccaccatagt gaaaaaatga
    30721 acttttctga tttttacaga aatccagcca aacatattaa atatcgtaaa cgcagaatga
    30781 aataccagga aaaatccatc taacaatgta tacacaatct gcataatgaa gtatctcact
    30841 tattagcata atcaaaatat aactaccgtg ttcaaatgta acaattaatt ctcgcatacc
    30901 attttcattt atatatttca agtataaagt tcttgatatt catcagcttt catgtattca
    30961 atacatagtg tcataaggag tattgtatgc ttttatcagg cactatcgtg attgatatca
    31021 cacaatttat ttcaggttcc cgatgtacgc agatacttgc tgatatgggt gctgaagtaa
    31081 tcaaagtcga acatccgaaa ggcgacactc tccgccttat ttttcaattg atggctggtg
    31141 cagaacgctg ttactcagtg ttcaaccgaa ataaat
//
LOCUS       DAORYL010000030        30729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1254_length_30729_cov_14.225957, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000030 DAORYL010000000
VERSION     DAORYL010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 30729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30729
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1254_length_30729_cov_14.225957"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /gene="asd"
                     /locus_tag="PLV81_07295"
     CDS             complement(<1..839)
                     /gene="asd"
                     /locus_tag="PLV81_07295"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HOM09941.1"
                     /translation="MNISVKLIVFKILPTRILSRLFGYCTRIPLPAFIMKKIISWYSK
                     KFNVALDEAFIPPGGFKNFDEFFTRNLKRGMRSVQPHPLALVSPVDAMVSACGNINAG
                     QIIQAKGIKYDVASLLPGQFQSQFISGSFITLYLSPGDYHRIHSPVSGTIVGYQYDPG
                     KLYTVQDWLVKMLPGLFTVNERITSYIQSAFGLVAVCKVGAMNVGRISLSYAPVFTNH
                     CFAKPYFYRFDETSRPKVSKGDELGIFHLGSTVIVVTQKPVSFVPNIEGTKVQMGQVL
                     GYF"
     gene            1099..2181
                     /locus_tag="PLV81_07300"
     CDS             1099..2181
                     /locus_tag="PLV81_07300"
                     /inference="COORDINATES: protein motif:HMM:NF017770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HOM09942.1"
                     /translation="MESIEQTNTDSKIISFHFTGKASEYFKIWIVNGLLTVLTLGIYS
                     AWAKVRTNRYMYGNIYLDNEPFAYTAMPVNILKGRLLALAFFVVYSLSGQYSQTLYMI
                     LTLSLLPIIPWVIVNSIRFRGKYTLYRNIPFIFKGDYLDAFKYFILIYIWVPLTLGII
                     IPYIYYKQKAYIINNLYFGILPVKYNGKASTFYIAFIASAIITLLSAGVIVGIAMALG
                     SSGFFELLKKNIEHTASINVPMIATVSGIYVLLIISFFIGSAFYKIFILNNVISSIYC
                     GQSQLSCSMDYIKYISIFLTNALAIIFSLGLAIPWARIRYNKYFYSSISISLKDNLDE
                     ITSIDEKDIKSFAEELGGFLDLDIGF"
     gene            2159..>3162
                     /locus_tag="PLV81_07305"
     CDS             2159..>3162
                     /locus_tag="PLV81_07305"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOM09943.1"
                     /translation="MTLTSGSNITGTLYNKGISTPHTATLYYFDNGDIIIETESTTKK
                     LHISALSISSRVANTQRQIFMPDGDIFTTHDNNAVDTMIAKISPEKKDTMQSLLYYLE
                     SHVAAILSLIAVSIIAVFIFIKFIFPTIVTYIAFSIPASTLHLISEKTIEYLDNQFFT
                     QTQLSQTRIHTINAQFKEFIASIPEKNKYTFTLYFRHSNFIGPNAFALPDGTFIITDD
                     FVKLIQHKHELFTILAHETAHVVYRHGVRNILLNSGLLVIAIAIFGDVVSTTTIGLPL
                     LLIHNGYSREFERQADAFAIDYCKKNHISGKHFVNLYKRLQQKAPTNIPSLLSTHPSD
                     "
     assembly_gap    3163..3262
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3614..3847
                     /gene="yidD"
                     /locus_tag="PLV81_07310"
     CDS             3614..3847
                     /gene="yidD"
                     /locus_tag="PLV81_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOM09944.1"
                     /translation="MYNAAQAIGYFFIRMFQVVISPQDGPNCRFTPTCSEYGRQAVMR
                     YGLLLGGFLAGDRLIRCNPYTIPQHDPVPQHLR"
     gene            3853..4665
                     /locus_tag="PLV81_07315"
     CDS             3853..4665
                     /locus_tag="PLV81_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09945.1"
                     /translation="MIIVPLIITLLFNDTGSHQNFIAALYSQQEYYQCIAELRRYSLA
                     TGADMDYSIAFCYYKGKRFSEALTVLKSKPDKDSKHYLLLANIYTALQQYTDALQTLY
                     MCDSNRDNQMQLLLYKNILTVHVAHYDWDNALAFYNSHSDTLKDMRDIFSLLNKAKQE
                     AKNPYMAAVLSAMVPGSGHVYAHHYTDGIISFVTVAALAGATYLSYAKGNGGLMYTFG
                     TLTTIGYAGNVYSAYTSTLYSNETMNSNYRTHIQNQFLQYSLEIFLPEWMKQ"
     gene            4662..5507
                     /locus_tag="PLV81_07320"
     CDS             4662..5507
                     /locus_tag="PLV81_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09946.1"
                     /translation="MKKIIVFMIYSIVLMRPLFANPLIDLSKTFLKNKEYYHAITECM
                     RYQYCYPNGEHYPESMLIMAHAYFWGGNYYRATDIDYDCYQKFSNTQWGQQALYNLAT
                     MRLLEGSAFFAYRNFQEYFSMYPDGSLKEQATLNFIYTEVMLHYYSDAQKDLQQYIAT
                     GNNEELRNEAITFAKTLNDYQKKPKKLVWLSVAGSLIFPGFGHVYTGNYATGILSFAS
                     TALCAALAYRGLKHDNTFQLVFFGLAGFTFYQYSLVSAVVNVYEYNSDKQLKEAIKLS
                     VMKRF"
     gene            5811..6410
                     /locus_tag="PLV81_07325"
     CDS             5811..6410
                     /locus_tag="PLV81_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09947.1"
                     /translation="MEQEKKIKDIETLLKERRPLEDIAQDILDGAFGELDMERKDSLD
                     RFLDFVYSKVQRGNPFIIHLAYPTKRMIDSELEAKVKELINEHLYPDIILPLLKFFTR
                     NVHNSDTNLYIAYLIENENIIKAIYETYLLFKKDIFETDKDKRTQNVRRMQQFLARID
                     TTSASPLDAAARLKFILEFFALKKNVTHIYTLDNVKLSH"
     gene            complement(6407..6871)
                     /locus_tag="PLV81_07330"
     CDS             complement(6407..6871)
                     /locus_tag="PLV81_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09948.1"
                     /translation="MKKKIYQDEVLYTIAWSPVYKFDKYSITKIIPELAGIVAIMEKL
                     NEETYHYILFYSCWRDGMRLGLKNLLDPIFTRHPDLAKAMEGRELWYKYTAVDSNPFD
                     LQDILWWLIQNYEPELNDIANYEDSKRYKNIYVREMELGKETVVERLPKFGF"
     gene            complement(6874..7446)
                     /locus_tag="PLV81_07335"
     CDS             complement(6874..7446)
                     /locus_tag="PLV81_07335"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOM09949.1"
                     /translation="MRIFYSYNKVLFPHCGITLPVKTKWLANFKPGDTVIVYPVKRLK
                     DYVHLHNVGVLAEVLKVELSNKQYLLSFKGLNRVRIDKRHGLWSCSYTMLDEKVHVNA
                     DITEKLRKKAQELIFLINVDESDKLIHLLNFISDGVQLSDFIANYFILDYNERVALLN
                     TTVVTSRINTILQLLTMLIDNIQHKRRSEG"
     gene            7626..7940
                     /locus_tag="PLV81_07340"
     CDS             7626..7940
                     /locus_tag="PLV81_07340"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOM09950.1"
                     /translation="MAEKRKYNRVSKKCKSVVESMEGVTFSTAHDISEGGMFISTPEP
                     VHPGSTVDIEMYISPEETITLKGTVRWVRKHEEGDKKAGMGIQFIPTTQEIEKLKKAM
                     LK"
     gene            complement(7932..10670)
                     /locus_tag="PLV81_07345"
     CDS             complement(7932..10670)
                     /locus_tag="PLV81_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOM09951.1"
                     /translation="MQQVHLPLDEEYSSVQDTASSDAVILDYIAQHSFVPTKRFLLKT
                     ESFVLSSAYVNNKMLSLSNSRTRILPHQIESTFTVVNSLHKRFLIADEVGLGKTIEAG
                     LIIKELSYRYNYDRVCIVCPASLLYQWQHEMKSKFNEDFTIVDKKIYTRYKGNPWVLN
                     KIICSIDFIKNRSFIETLSKVQWDTVVIDEAHRLRRDAATQTKSYQVAEILAKNSKAL
                     LLLSATPFRGKLEELYYLLTLLDKNLLGPVHSFQATYCVPDADLAPLKEKIAQVVIRR
                     TKREVGGFTKRLAKTIRFELYPEERMLYDATTQYVADQFNKAMQTENRAIGFVMTVFQ
                     KLLDSSSYALLCALKKRHEYLTGILVQKKASAYDDSVLDEDAIEDVDTVVQVESVELE
                     EELTTLAKLIAMAQDVTRNKKAEKLVQLVKKLKAQNHKKILIFTQFRTTQDYLAQVLK
                     SYKVVLFHGSLSREEKEQAIECFKSESEILICTEAGGEGRNLQFCNVLINYDLPWSPL
                     RVEQRIGRIHRFGQPQNVIIYNFATKNTVAERVLEVLNHKLKLFEESLGTPDVILGDI
                     ADERFFNSIFMELIAGLKSKKRVTKDIDTHIQKAKSSYSKLQELTVAKKIDLNFDEYY
                     KITMQERAFSNKRIEQFINELKEVDASISKCISGPNPKNKLYFVRYNPVTHTPVNRYG
                     TFTSEVALENESLEFLAFGHPVIDFCIEQCQEHAFGGLAGIKRIDCPYDCSGLLFHYL
                     VTLKAKEEVQRIIPVFVSKDILNQSIIDDIEQETLRQYGCAVSVADDVAREYSSNIYH
                     YYELSLKRLEKKVGAIVTEIKDNMNLHIDPYMERVCQHYQQEKEYLHKRLEWQECQQK
                     WYGKDMQSAITRTKNMIQKIDEEHHNRLKQYSGFTDVTWDASLVNAGILVCTPGSF"
     gene            complement(10827..13817)
                     /locus_tag="PLV81_07350"
     CDS             complement(10827..13817)
                     /locus_tag="PLV81_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09952.1"
                     /translation="MAVTEEIQNLTPEEEAEITRIVDSFDAELASIAQMRGRVSVIQA
                     DSSAADFTITPGQREEEGEGLTTKEVYEESEEIVDITDMIEEVPEEMPPSVEEALPEF
                     EEHVSEEALPQEPEKAFEETLPEELPEEIPSFEEKELPPKPPKKEVSPLDELEELTKF
                     EPETVEAHEIRTDEFVTEEPRIPRDVSLDEDIHLSEIGTEEVSEISEIKAEELPEADL
                     TTLGLDEEIPEPSAFEEVSKEETPRARFETPSFDELESFEEPVRKAEKVESVVEEFKP
                     EFEGLEEEEPQPISMEESVEISDAELRRLKKAMLLLHPVLRRKIKEIILRDELSPEDT
                     RKLIDLILSGKPEDNIQRFIEKKLNIKLTLEEEIPHRRVVTARPEYTREGMQRQQRLL
                     RQTKIIAAAVLIGFALTVTVYQFIYKPFMAKRLINKGVALILEPGDPATKKVKDYKKA
                     EEYFEYVDQHYKKDYLYGYNAYARAYFKKREFDYAYSKLKRAFEINSRDIDTLNNTGY
                     FFAKIPQNYYSRIQNDALRTFAPKGTYEMSQVDMAIMMYNRVLAIDPKNITAMFGVGN
                     AYLYQGQYLKSRQYFENILKVDPNSWVGYAGLLNLFIERDNLPEVLSIHTDLRARDIL
                     TDIPSPLLAKLAHYYLGKNRSDTFNVRVDLGIQSPRLKDEEDNLVPACRGVLDALKEK
                     DPDYPPLYVASAKLAAAQKNLKLVEMLLKEAEQKAEEKGERYAAALQLLGEYNYTIKQ
                     PVEAYKYLNAAITAYQYPPEFTYEDFYYESESIGKAYAYLGHIFYYFFDKVKFRFGDE
                     LDEALLDEDADKMANLDIARQKYELAIQENFSSPEVRYNLGRIYYVNQNYQQALEQWL
                     NLYEDIVTNPQVMFAIGNAFYHMNNLEASKGEYLKMIAVFERKAESIRLPRPERKEHR
                     IIFQSLASAYNNLGAIYQIQGSETKSSIAYWKAIDYASRLGMENEFARVNLARGFKPR
                     TTPIMPILDENIPYSIDIFRADMR"
     gene            14022..14753
                     /locus_tag="PLV81_07355"
     CDS             14022..14753
                     /locus_tag="PLV81_07355"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HOM09953.1"
                     /translation="MPKPLQWIMLIISLCYFSSSDYSPAKSVPYYSLYTLSKAMPPSP
                     VTVSHSKPGMPQILRGTLITYKNRSAHRVYIAGDFSYWKKQPMERSNNGVWFYIIPFN
                     DDDRVVRYKFLVDDVWINDPLNHDIADDNAGSYVSIVRQDKLSEGTQVSYKILDESIR
                     LVEFRLYAPKASIVALAGDFNNWNPEVDLLQKDKNGIWRLQKRLPKGAFRYNFVVDGI
                     WKLDLYNPHTASNAAGMLCSVIQFQ"
     gene            complement(14795..14965)
                     /locus_tag="PLV81_07360"
     CDS             complement(14795..14965)
                     /locus_tag="PLV81_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09954.1"
                     /translation="MRTNAPRSIVWWISVVIGVLGIIGKFIALPVLTAYSWWLVAIGF
                     VILALATVIKGL"
     gene            complement(15264..15905)
                     /locus_tag="PLV81_07365"
     CDS             complement(15264..15905)
                     /locus_tag="PLV81_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007044468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOM09955.1"
                     /translation="MHIVNKIYQQLLSHHGPQHWWPGETQFEVTIGAILAQNVSWTNA
                     AKAIENLKKNDLLQPQKLFKKKPEAIAPLIKSSGYYNQKAAAIATFLRWFKQYDFSFE
                     ILQKLPTETLREELLALLRIGPETADSILLYALERKIFVVDSYTKRIFTRLGILTGNE
                     DYTAIQKMFHQNFEGAVHDYNEYHALIVKHGKDICTKKPHCDMCCIDNICKTV"
     gene            complement(15909..16415)
                     /locus_tag="PLV81_07370"
     CDS             complement(15909..16415)
                     /locus_tag="PLV81_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOM09956.1"
                     /translation="MKPYSIDKLGEDILEILSNDAKATPEDIAKQLNVSVDKVQKYIK
                     KLEKDRIILRYKTHINWQRIKQHEVRALIEVKVTPQRGVGFDSVAEIIYRFPEVTSVY
                     LLSGGYDLLVLVEGPSLREVASFVAEKLATIDHVQSTVTHFMLKKYKEDGDILVDQPE
                     GKRLPVSF"
     gene            16605..17384
                     /locus_tag="PLV81_07375"
     CDS             16605..17384
                     /locus_tag="PLV81_07375"
                     /inference="COORDINATES: protein motif:HMM:NF016873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HOM09957.1"
                     /translation="MKKPYLFIIPHGGYKVPDELAELCMLSQLELFFEADTGATECFK
                     LKELGTVISTPVHRLFIDVDRDPYMLPPKSNNGVIKTSTSQKKQIFPAGIFPDEIAIA
                     GMIKRYWFPFHSRIEQALGTSRFIVVCHTVAPVAPPVAADAGSPRPIITLEHITSNGS
                     DITTCDETLTVKLLGILQKAFAGEKYTVTTPFCIGRPAKRYIMYKYAKHLLPIIRISL
                     SKALFVNEKYFDPQTLTIDPERIQGIQQRLYECFEAFIRFM"
     gene            17672..17875
                     /locus_tag="PLV81_07380"
     CDS             17672..17875
                     /locus_tag="PLV81_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09958.1"
                     /translation="MNAVKSIYKNAPSSIPVPKEFQKKNIEVIFLPLDEEKKSIDVSK
                     FFGAIPDFPERAPQGELQTRENF"
     gene            17875..18273
                     /locus_tag="PLV81_07385"
     CDS             17875..18273
                     /locus_tag="PLV81_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006669410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOM09959.1"
                     /translation="MNYLLDTNVCIQFITGRSIALKEKIEAADISSLFICSIVRGELE
                     YGARKSNNPEKTLTILKEFLACFPEISFDSAAAECYGIIRAELEKQGTPIGPYDMQIA
                     AIALAHNFTLVTHNIKEFQRIKNLRIEDWE"
     gene            complement(18314..19231)
                     /gene="trxB"
                     /locus_tag="PLV81_07390"
     CDS             complement(18314..19231)
                     /gene="trxB"
                     /locus_tag="PLV81_07390"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOM09960.1"
                     /translation="MKELIIIGAGPAGMAASIYAMRAGLDTLVLEKISPGGQVMLTYE
                     IENYPGFVEPVEGWQLASNMEQQARRLGVTIESKDVEQIRKVQDVFEIKTTDGSVLTA
                     RSVIAATGASLKRLGVPGESEFTGRGVSYCATCDAAFFKDMVTAVVGGGNTALEEALY
                     LTRFASKVYLIHRRDEFRGQKILQDRVRSNPKIELVLDTVVTQVIGDEKVKAISLLNK
                     KTNKTSELTVNGLFIFIGFSPNTAYLPKEVLNEAAQVKVDMHMRTPIGGLFAAGDLRE
                     GSYRQVLMAAADGATASISAYEYITNKEW"
     gene            19610..20821
                     /gene="icd"
                     /locus_tag="PLV81_07395"
     CDS             19610..20821
                     /gene="icd"
                     /locus_tag="PLV81_07395"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HOM09961.1"
                     /translation="MEEIIKYENGNIEVPDNPVVLFIEGDGTGPDIWRATKIVLDAAV
                     KKAYGGKRTIQWQEILAGEKAFNMTGSWLPEETLEKIKKYKVAIKGPLTTPVGGGIRS
                     INVTLRQVLKLYACVRPVRHLAGVPSPLKNPEKVDMVIFRENMEDVYAGIEWKQGTPE
                     AKKIIDFLNNEMGQSLSYDTGIGVKPISIENTKNIVRAAIRYCIDNNRPSVTIMHKGN
                     IMKFTEGAFRDWAYQTAKEEFARYIITEDELWSTYNGKVPAGKIVIKDRIADALFQMV
                     LLRPDEFDVIVTPNLNGDYISDALAAQVGGLGMAPGANIGDNAAVFEATHGTAPKYAN
                     QDKVNPGSLILSGEMMLRFMGWKEAADLIIKGLEKTIQQKRVTYDLERQMEGATLLKC
                     SEFGQAVADNM"
     gene            complement(20823..21026)
                     /locus_tag="PLV81_07400"
     CDS             complement(20823..21026)
                     /locus_tag="PLV81_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09962.1"
                     /translation="METVSGVLCEVCHAKEAVVRCDGCGKALCKECRVFDIWCYGCGH
                     GNTKAFCKKCNDDPAINIWKTPV"
     gene            complement(21055..22626)
                     /locus_tag="PLV81_07405"
     CDS             complement(21055..22626)
                     /locus_tag="PLV81_07405"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOM09963.1"
                     /translation="MAKNSELILSIDAGTQSIRAIVFNLQGNVVDIVKTPIEPYFSNY
                     PGWAEQDPEYYWKTLCTTTKKLFAKGKVKKDAIRGVTLTTQRDTVINVDKNGKPLRPA
                     IVWLDQRRADKSIVKLPWYIKAGLRLVNMLDAVEYFITEAEANWIRQYQPEIWEKTYK
                     FLLLSGFFTYRLTGDFVDSTGCQVAFIPFDYKTQDWAPASDLKWKLFPMSRDTLVRLV
                     KPTEVLGYITKKASNETGIPQGLPLIAGAADKACEVLGSGCLTPNIACLSYGTTATVE
                     TTNTQYVEIIPFFPPYPSAVPGSYNTEVMVYRGFWMLSWFVKEFGAREELQAKKMGIK
                     PEALFDRLVKEVPPGSMGLTLQPYWSPGVKIPGPEAKGAIIGFGDVHTRVHIYRAILE
                     GLAYALREGAERTQKRNKVPIEKLRVSGGGAQSDVAMQLTADIFNLPAERPHTFETSA
                     LGAAIDAAVGLKMYPDFKTAVSAMTRVGKVFYPNPKDVAIYDKLYKKVYKRLYNRLQP
                     LYQDIRDITGYPEKR"
     gene            complement(22767..23213)
                     /locus_tag="PLV81_07410"
     CDS             complement(22767..23213)
                     /locus_tag="PLV81_07410"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOM09964.1"
                     /translation="MVETTQGLDDIFTLIESKLKGFIVNYNAHNYRECVTALFHVAEG
                     LVRYVLATRGYFPISHEGIQVLLAQHFIKPGSVKKNVYNYLTNLYMRRKDADYKGFVS
                     FDSDDIKIYWQWVLEIVDEFQSFLDEIHKQKIDELVTEGKTLVLKK"
     gene            complement(23189..23518)
                     /locus_tag="PLV81_07415"
     CDS             complement(23189..23518)
                     /locus_tag="PLV81_07415"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOM09965.1"
                     /translation="MSKLTPYEEKLINDFVEYITSVPYVNSIVVYGSRSKGVSNEYSD
                     VDIAVVIDNLHNSKKVEKLIEQWNIDNSPEILVHFLVIDTNNLHSSAIGKEIEKGDTV
                     WLRQRKG"
     gene            23650..24393
                     /locus_tag="PLV81_07420"
     CDS             23650..24393
                     /locus_tag="PLV81_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOM09966.1"
                     /translation="MFTLIETKPVADDHYLLTLHCDAKESIPGQFINIKVNTSTDPLI
                     RRPFSIFDHDQTTIHIVIRVVGKGTERIVALHPGPVDCIGPFGKGFSVVTGKKALLVG
                     GGVGNAPLYYLAKMLRRVNTQVTYIYGSRSASHIFLLDRYQELSHTCIVTTDDGSLGI
                     KGNPIDVAKDIVAKHKFDIVYTCGPEIMMKRLSRELSHIPIEASLERYFGCGVGLCSG
                     CAVATINGYRRACIDGPVFDAHTVLWDTP"
     gene            complement(24395..24958)
                     /locus_tag="PLV81_07425"
     CDS             complement(24395..24958)
                     /locus_tag="PLV81_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOM09967.1"
                     /translation="MDITKFYITPDNTALFIIDVQDSLFNSMEYEMRKKVAKNVGILI
                     ELAKQYSLPIILTEQYRKGLGPTIKELMDRLEGVVAFDKLAFDCMKDNTIREQVTRIK
                     RTNIILCGIETHICVLTTALSLLKENYNVIIASDAVCSRRTHEWEMAIHALRQAGAIV
                     YPTETISFMILEKAGTERFKQLSPLFK"
     gene            complement(24964..>25377)
                     /locus_tag="PLV81_07430"
     CDS             complement(24964..>25377)
                     /locus_tag="PLV81_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09968.1"
                     /translation="TPQLVTGIWMGYDGKGLSLGPGQSGGAVVAPVWGQYMSCALQKE
                     PVLDFGVYGNITTVKVCAESGLLPSYSCKKVIDELFIPGTEPQEECTKCATGSPLPTT
                     KIPHINVSEKQKEQILKKMKKKDDTILDSIDNMIR"
     assembly_gap    25378..25477
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25478..27120)
                     /locus_tag="PLV81_07435"
     CDS             complement(<25478..27120)
                     /locus_tag="PLV81_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOM09969.1"
                     /translation="MRRYQVNRKFSKKSYINAGDFFVRIINFFEDILYALELRFGNVR
                     PIIIGIIAGAVIAFTVILVIDFYKVRALAHYTPYQTTKIYDKNNVLVAEFFREKREVV
                     PLKRIPPYVVQAFIAMEDNEFYEHFGINPKGIVRAFFVNLVSGRVKQGGSTITQQLAK
                     ILLTSGERSLYRKVKEAFISLMIELFYTKDEIMEMYLNQIFLGHGTYGVESAAKFYFG
                     KHVWDLNLAECSLLATLPSAPNVLSPLKNPRLSMERHRVALAKMVDMGFITVPQAEKA
                     YLDFWPDYLAYINDLPPTMTTWSSRVNKAPWFTEHIRRILVAKFGEKAVYEDGLTVYT
                     TLDVDKQQCAQRLLKDAVEKQTVISRSLAFDNDEYVSNRFADEVNLLNMLFGLPAIKS
                     SGSAEKRKFNRYMKDGLLWELQALNLIAGLDTVEDALQQYFEGSWDQKEFMNVEGCLI
                     SINPTNGYIEAMVGGSDFTSTNQLNRVMQSKRQAGSAIKPLLYTAAIDSKKFTAATTI
                     MDSPILYLDLEGGDWIPENYEGEYYGLVTLRRALALSINV"
     gene            27320..28153
                     /gene="dapF"
                     /locus_tag="PLV81_07440"
     CDS             27320..28153
                     /gene="dapF"
                     /locus_tag="PLV81_07440"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOM09970.1"
                     /translation="MPFTKLHGIGNDYIYINAIKYPVKNPRKLAIAMSDRHFGVGSDG
                     LILILPSQKADFRMRMFNADGSEAEMCGNGIRAFAKYVYDHKLTKKTHISVETLAGIK
                     YLQCTVKRGKVHTVTVDMGEPILLRDKIPMIGNPGMVIEEEIHVDGVKFPITAVSMGN
                     PHAVIYVEDVKNFPVEKYGPMIENHELFPNRTNVEFVEIVNEKEVIQRTWERGSGETL
                     ACGTGASAVTVAGVLTKKTSRKLNVHLSGGDLTIEWNEKDNHVYLTGPAEEVFEGVWP
                     E"
     gene            complement(28157..28804)
                     /locus_tag="PLV81_07445"
     CDS             complement(28157..28804)
                     /locus_tag="PLV81_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09971.1"
                     /translation="MNHKRSFDKLKTAYKKYKGKTWQLYNTLIEQCRHYFTAFIELVV
                     NPPADTKKRYRMVLLAVSILCIIDYAAISVLTDKNIFDIYPSLPALDTRETIDIFVAD
                     LDAKSILKETRQVLLPQNNESKVRRLVKLVAEGSEFENTRAIAPIDIVVRHVWFYDTT
                     CIIDCELGTIEGQVPYIPDSFKAFNKALEQTISRNIPGITRVVLLEHGIYGKNLW"
     assembly_gap    29025..29124
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<29125..29936)
                     /locus_tag="PLV81_07450"
     CDS             complement(<29125..29936)
                     /locus_tag="PLV81_07450"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOM09972.1"
                     /translation="MLKSRDIVKRITRCVILLLNIAFIPVYSDIYSQDSIALPVKIVS
                     GNAYISEYDIRTLLPVQSSFDILLQRGKIMRGPRFAIYTVGMSVALSGDMLLRSEYPV
                     TRMQGEVLIPLDIALPMIENLLEGYAVAIQGNSIVIKKEAVVPQEKQKEKKPLPHVSK
                     DAIGFIVVDAGHGGKDPGAIGKSGIKEKLLTLQIAREVALELQKKLPGIDVVMTRTTD
                     KFLELGERTEIANRLLKKGVNGIFVSIHCNAAVVSKISGFETYYLSQNPSNE"
     gene            complement(29954..30544)
                     /locus_tag="PLV81_07455"
     CDS             complement(29954..30544)
                     /locus_tag="PLV81_07455"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOM09973.1"
                     /translation="MRIKDLLEGRVATWSNLISVVRVLLAPAIAVALYYERMTGDIWY
                     CKVAVALYLVVAVSDFIDGMVARLLHQVSRLGQFLDPVADKCVILIVAVTLYYFRQFP
                     LWMIVLILIRDITHIIAGFYLFYAHDIQVRPSIAGKCMVVIMGLCGLIFIVNPLHTFA
                     GYTLQQISLLLVVLFMVVSSISNWRIYSKFYFTGKR"
BASE COUNT         9067 a         6449 c         5525 g         9388 t
ORIGIN      
        1 taaaaatacc ccaatacctg tcccatctgc actttggtgc cctctatatt gggaacaaag
       61 ctaacaggtt tttgcgtaac tacaatgaca gtggaaccta agtggaaaat accaagctca
      121 tcaccctttg atacttttgg tcgactggtt tcatcaaacc tgtaaaaata tggctttgca
      181 aagcagtggt tagtaaacac cggtgcatag cttaatgaaa tcctgcccac attcatggct
      241 ccaaccttgc atacagccac caacccaaag gctgattgta tatatgacgt aatgcgttca
      301 tttacggtga aaagcccggg caacatttta accagccagt cctgtaccgt atacagtttt
      361 ccgggatcat actggtaacc aacaattgta ccacttacag gtgaatgtat gcggtgatag
      421 tcaccgggag aaagatataa tgtgataaac gatccactga taaactggct ctggaactgc
      481 cctggcaaca gtgatgccac atcatactta atgccttttg cctgaattat ttgcccggca
      541 ttaatgttac cgcatgcact taccatagca tcaacgggcg atactaatgc aagcggatgt
      601 ggctgcacag agcgcatgcc ccgtttcagg ttacgggtga agaattcatc aaagttttta
      661 aaaccaccag gagggataaa agcctcatcc agcgcaacat taaacttttt actgtaccag
      721 cttatgatct ttttcattat gaaagcaggc aagggtatac gagtacaata tccaaacaac
      781 cgcgacaaaa ttcgcgtggg aagaatttta aacacaatga gtttaactga tatattcaaa
      841 tgagcctcct gattgcagta tgtaaaatat ttctattcat catcgttagt acaagccaaa
      901 ccatccttca acgaagagtg taaatataaa gcatactact tttaaactat gtaagtattt
      961 gagaagtatc aaatagtata ttgtcaatca gttttcaggt gtttatttac tattctacca
     1021 ttatttatgt cttgacttgt ttgaatttat atagtactat actgcgcatt gaattttata
     1081 ttttaacagg ggacttttgt ggaatcaatt gaacaaacaa acactgacag taaaatcata
     1141 tcattccatt ttacaggtaa agcatccgag tactttaaga tatggattgt taatgggttg
     1201 ctaacagtat taactctggg catttattct gcatgggcaa aagttcgtac aaacaggtat
     1261 atgtatggca atatatacct ggataatgaa ccttttgcat atactgctat gcctgttaat
     1321 attttgaaag gaagattgtt agctttagcg ttttttgttg tgtattcatt atccgggcag
     1381 tattcgcaaa cattgtatat gatcctcaca ctatcattat tgccaattat accatgggta
     1441 attgtaaaca gtatacgatt tagaggtaaa tatacccttt accgaaatat cccttttata
     1501 tttaaaggtg attatctgga tgcatttaaa tattttattt tgatatatat ctgggttcca
     1561 ttaacactgg ggatcattat tccatatatt tattacaaac aaaaagcata tattatcaac
     1621 aatctgtact ttggcatcct cccggtaaaa tataacggta aagcttcaac attttacatt
     1681 gcatttattg cttctgcaat tataaccctt ttatcagcag gtgtgatagt ggggattgca
     1741 atggcattgg gttcatcagg attttttgaa ttgctgaaaa aaaatataga acatactgca
     1801 tcaattaatg tacctatgat tgcaacagta tctggcatct acgtgttact gataatttca
     1861 ttctttattg gctctgcctt ttataaaatc tttattttaa ataatgttat atccagtatt
     1921 tactgcggtc agtcacaatt atcctgctca atggattata taaaatatat atccatattc
     1981 ctgaccaatg ctcttgccat tatattttct ttaggattgg caatcccatg ggcacgtatt
     2041 cggtataaca aatattttta ttcttccatt tctatttccc tcaaagacaa tcttgatgaa
     2101 attaccagca tagatgaaaa agatattaag tcatttgctg aagaattagg aggtttcctt
     2161 gaccttgaca tcgggttcta acattacggg tactttgtat aataaaggca tcagcacccc
     2221 tcacacagct actttgtatt attttgataa tggtgacatc atcattgaaa cagaatccac
     2281 cacaaaaaaa ctacatattt ctgctctcag catatcatcc cgtgtagcta atactcagcg
     2341 gcagatcttt atgcctgatg gtgatatatt cacaacacat gacaataatg ctgttgatac
     2401 aatgattgca aaaatttctc ctgagaaaaa agataccatg caatccctcc tctattacct
     2461 tgaatcacat gttgcagcaa tattgtctct tattgcagtt tcaattatag cagtttttat
     2521 ttttataaag tttattttcc caacaattgt aacatatata gctttttcta ttcctgcaag
     2581 cactcttcac cttatttctg aaaaaaccat tgagtatctt gacaaccagt tttttacaca
     2641 aacgcagctt tcacaaacac gtatacacac tataaatgca cagtttaaag aatttattgc
     2701 ctccatacct gaaaaaaaca aatatacctt taccttatat tttagacata gcaattttat
     2761 tggccccaat gcttttgccc ttcctgatgg tacttttatc attaccgatg attttgtgaa
     2821 attgatccag cacaaacatg agcttttcac aatacttgct catgaaacgg ctcatgtggt
     2881 ttaccgccac ggcgtacgta atatactgct taattctgga ttactggtta ttgccattgc
     2941 aatttttggt gatgtggtat ccaccactac gataggtctt ccacttttgc ttattcataa
     3001 tggctattca cgtgaatttg aacggcaggc agacgctttt gcaattgact attgcaaaaa
     3061 aaaccacata agcggaaaac attttgttaa cctgtacaaa cgactgcaac aaaaagcacc
     3121 aacaaatata ccatcattgc tttcaaccca cccatcagac gannnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnttgtcatt tcgacgaacg cagtgaggag aaatcttatt
     3301 ttcacattgt catttcgatg aacgcagtga ggagaaatct tatctaatta ctttgtcatg
     3361 ctgagtccca attgtattgg gatgaagaat ctcaaagcta taaacgtccc ggcaaagtcg
     3421 tgattcctat gaatgacggg gtaaattagg agcgataatg aaaatgaaaa aaaccagtat
     3481 accatgtcac ctgatactat ttttcgtggt tatgctgtgt gtaattacaa cccctttgtt
     3541 tgctgacttt cccccatggg acgccgatgt ttccgtagca aacgaaaccc gcgtgcagag
     3601 gcgcacaccc actgtgtaca atgctgccca ggcaattgga tattttttca tccgcatgtt
     3661 tcaggttgtt atatcgccac aggatggtcc taactgtcgc ttcacgccaa catgcagcga
     3721 atacggcagg caggcagtga tgcgctatgg tttactttta ggcggttttc ttgccggtga
     3781 caggctcata cggtgcaacc catacactat tcctcagcat gatccagtac cacagcattt
     3841 acggtaacca ccatgatcat tgttcctctt atcataacac tgttatttaa tgataccggt
     3901 agccaccaaa atttcatagc tgccctttac agccagcaag agtactatca gtgtattgca
     3961 gaacttcgtc gctacagttt agcaacaggc gctgatatgg attacagcat tgcgttctgt
     4021 tactataagg gcaaacggtt ttctgaagcg cttactgtgt tgaagagcaa acctgataag
     4081 gattcaaaac attatcttct actggcaaac atctataccg cactccaaca gtatactgac
     4141 gctttgcaaa cattgtatat gtgcgatagc aaccgggata accagatgca attactcctg
     4201 tataaaaata tacttactgt gcatgttgct cactacgatt gggataacgc tctggcgttt
     4261 tataattcac acagtgatac actaaaagat atgcgcgata tcttttcttt gctcaataaa
     4321 gccaaacagg aagcaaagaa cccgtatatg gccgcagtat tatcggcaat ggtgcctggt
     4381 tcaggtcacg tgtacgcaca tcattataca gatggcatta taagtttcgt aacagttgcc
     4441 gctcttgcag gtgcaacata tctttcatat gcaaagggta atggggggct tatgtatacc
     4501 tttggtacgt taactaccat tggctatgca ggcaatgtat attcagcata taccagtacc
     4561 ctttatagca atgagacaat gaacagtaac tatcgcacac atatacaaaa tcaattttta
     4621 cagtacagtc ttgaaatatt tttacctgaa tggatgaagc agtgaaaaag attatcgtat
     4681 tcatgatata tagtattgtg ttgatgaggc cactttttgc caacccactg attgatttat
     4741 ctaaaacttt tttaaagaac aaagaatatt accatgctat caccgaatgt atgcgctatc
     4801 agtactgcta tcccaatgga gagcactatc ctgaaagcat gctcatcatg gctcatgcat
     4861 atttttgggg aggcaactat taccgtgcca ctgatattga ctacgattgc tatcaaaaat
     4921 tttccaacac acagtggggg cagcaggcac tgtacaattt agccaccatg cgccttctgg
     4981 aaggatcagc gttttttgca taccgcaatt tccaggaata tttttccatg taccctgatg
     5041 gcagtttaaa agagcaggca acactgaact ttatttatac tgaagttatg ctgcactact
     5101 acagtgatgc tcaaaaggat ttgcagcagt acattgcaac aggtaacaat gaagaactgc
     5161 gcaatgaagc tataaccttt gcaaaaacgt taaatgatta ccaaaagaaa ccaaaaaagt
     5221 tagtgtggct ttcagtagca ggttctctta tttttccagg ttttgggcat gtatatactg
     5281 gtaactacgc tacagggata ctgtcatttg catcaactgc tctctgtgct gcccttgcat
     5341 accgcgggct caaacatgat aatacttttc agcttgtatt ttttggactt gcaggtttta
     5401 ctttttacca gtattctctg gtaagtgctg ttgtcaacgt gtatgaatat aacagtgaca
     5461 aacaacttaa agaagctatt aaattatcag taatgaagcg gttttagaaa tattaagctg
     5521 catgcatcac tgcggtggtc actgaatacc ccatacatca tttataaaat attttacttg
     5581 ctaaaattat tttatgaaat tacattgtaa ttacaataaa taatcaaggg caaacactat
     5641 gagaactaaa ataataaaaa ttggcaactc aaaaggtatt cgtataccca acactattct
     5701 taaagtactg ggcaacactt caaccgtgga tatgctgttt gatgaaaaag aaaaataata
     5761 tctgttttgc atgagatgct tatagaataa aatattgacc atcaagatcc atggaacagg
     5821 aaaagaaaat aaaggatatt gaaacgcttt taaaagagcg gcgccccctt gaagacattg
     5881 cacaggatat tctggatgga gcgtttggcg agcttgatat ggagcgcaag gactcccttg
     5941 accgcttttt agattttgtg tacagcaagg tgcagcgagg aaatccattt attattcacc
     6001 tggcatatcc tacaaaacgc atgatagatt cagaacttga agcaaaagtt aaagagctta
     6061 ttaacgaaca cctttaccct gatattattt tacccttgct taaatttttt acccgaaatg
     6121 tgcacaactc agataccaac ctgtatattg cctatctaat agaaaacgaa aatataatta
     6181 aagctatata cgaaacatac cttttattca aaaaagacat ctttgaaact gacaaagata
     6241 agcgcactca gaatgtacga aggatgcagc agtttttagc acgtattgat accacttctg
     6301 cctcgccact tgatgctgct gcacggttaa aatttatttt agaatttttt gccctgaaaa
     6361 agaatgtaac ccacatttac acactggata atgttaagct ttcacattaa aatccaaatt
     6421 ttggcagccg ttcaaccact gtttctttgc ccaattccat ttcgcgaaca tatatatttt
     6481 tatagcgttt ggagtcttcg taattggcaa tatcattgag ttcgggttca taattttgta
     6541 taagccacca cagtatatcc tgtaaatcaa aaggattgct gtccactgct gtgtatttgt
     6601 accacaattc tctgccttcc attgcttttg ccaggtcagg atggcgcgta aaaattgggt
     6661 ccagcagatt tttaagccct aaccgcatac catcacgcca gcaactgtaa aataatatat
     6721 agtgatacgt ttcttcattc agtttttcca ttattgcaac aatacctgca agctcaggga
     6781 taatcttggt tatggaatat ttatcaaatt tatacaccgg cgaccacgca atggtgtata
     6841 acacttcatc ctggtatatt ttctttttca ttattatccc tcacttcttc ttttatgctg
     6901 tatattgtca ataagcatgg taagcaactg taatattgta tttatccgtg atgtaacaac
     6961 tgtagtattg agcaatgcca cgcgttcatt ataatccaga ataaaataat tggcaataaa
     7021 atctgaaagc tgcaccccat ccgagatgaa atttaaaagg tgtataagct tatccgactc
     7081 atcaacattt attaaaaaaa ttaactcctg ggcttttttg cgcagctttt cagttatgtc
     7141 cgcatttaca tgcacttttt catctaacat ggtataactg catgaccaga gcccatgccg
     7201 tttatcaatc cgtacacggt taagcccctt aaaagacagt aaatattgtt tattcgataa
     7261 ctcaactttc aaaacctctg caagcactcc cacattgtgt aaatgtacat aatcttttaa
     7321 ccgttttacc ggatacacta tcactgtatc gccaggcttg aaattggcaa gccatttagt
     7381 tttaaccggc agtgtaatgc cacaatgagg aaacaacact ttgttgtagg aataaaaaat
     7441 gcgcaaggta accacccaat tgtacttcat aaaattacgt atacagtaaa accactgtca
     7501 acatttgcaa tcattttatt tagtgtagat atataaataa cgctatcaaa aaaatttatt
     7561 gcaataatat tacacatact atacaataaa gccattcaac acaacaccac acaagcgagg
     7621 tatctatggc cgaaaagcga aaatataacc gtgtgtcaaa aaaatgcaaa tcagtggttg
     7681 aaagcatgga aggcgtaacc ttcagcaccg cccacgatat cagcgaaggc ggcatgttta
     7741 tatcaacacc cgagccggtt catcctggta gcactgttga tattgaaatg tacatttcac
     7801 ctgaagaaac cattacacta aaggggacag tacgctgggt acgcaaacat gaagagggtg
     7861 ataaaaaagc tggcatggga attcaattta tacctaccac tcaggaaatt gaaaagctca
     7921 aaaaagccat gttaaaatga acccggtgtg cacaccagta ttcctgcatt caccagactg
     7981 gcatcccacg ttacatcagt aaatccagaa tactgcttca ggcggttgtg gtgttcttcg
     8041 tcaatttttt gaatcatatt ttttgttctg gtaatggcgc tttgcatgtc tttgccgtac
     8101 cacttctgct gacattcctg ccactccagg cgtttatgca gatactcttt ttcctgctgg
     8161 taatgctggc aaaccctttc catatacggg tcaatatgca gattcatgtt gtcttttatt
     8221 tcggtaacta tcgcccccac ttttttttca agtcttttaa gcgatagctc atagtaatga
     8281 tatatgttgc tggaatactc ccttgctaca tcatccgcaa cgctcaccgc gcacccatac
     8341 tgacgcaagg tctcctgctc aatatcatca atgatagatt ggttgagaat atcctttgac
     8401 acaaacaccg gtatgatgcg ctgcacctct tcctttgcct tcagggttac aaggtaatgg
     8461 aataaaagac cactacaatc atagggacaa tcaatccgct tgatgccagc aagtccgcca
     8521 aatgcatgct cttgacattg ctctatacag aagtcaataa ccggatgtcc aaaagccaga
     8581 aattccagag actcattttc cagcgccacc tcactggtaa atgttccata tcggttcaca
     8641 ggcgtatgcg ttacggggtt atagcgcaca aagtataatt tatttttggg atttggtcct
     8701 gaaatacatt tgcttatgga agcatcaact tcttttaatt cattgataaa ctgctcaatg
     8761 cgtttgtttg aaaacgcacg ttcctgcatg gtaattttat aatactcatc aaaattaaga
     8821 tcaatttttt ttgcaacggt cagttcctgc aatttactgt agcttgattt agctttttgt
     8881 atgtgggtat caatatcttt cgttactcgc tttttgcttt tcagccctgc aatcaattcc
     8941 ataaagatgc tgttaaaaaa tcgctcatcg gcaatatcgc caagaatgac atctggtgtg
     9001 cccagcgatt cctcaaaaag tttaagctta tgattcagca cttcaagtac gcgttcggca
     9061 acagtgtttt tggttgcaaa gttatagatg atgacattct gcggctgccc aaagcggtga
     9121 attctaccaa tgcgctgttc cactctaagt ggcgaccacg gcagatcata gtttatcagc
     9181 acattgcaaa actgcagatt gcgcccctca ccaccggctt ccgtacatat taatatttca
     9241 gattcacttt taaaacactc tattgcctgc tccttttctt ccctgcttaa tgatccatga
     9301 aacaggacaa ccttatagct ttttaaaacc tgtgccaggt aatcctgtgt ggtcctgaat
     9361 tgtgtaaaaa ttaaaatttt tttatggttc tgtgctttca atttttttac aagctgcaca
     9421 agcttttcgg cttttttgtt tcgtgttaca tcctgtgcca tagcaataag ctttgcaagc
     9481 gttgtcagtt cctcttcaag ctcaacactt tcaacctgaa ctactgtatc aacatcttct
     9541 attgcatcct catctaacac gctgtcatca tatgcactgg ctttcttctg caccagaatg
     9601 ccggttaaat actcatgacg ttttttcaat gcacacagca gcgcatacga tgatgagtcc
     9661 agcaatttct gaaacaccgt cataacaaat ccaatggcac gattttccgt ctgcattgct
     9721 ttattaaact ggtctgctac atactgtgtg gttgcatcat acaacatccg ctcctcagga
     9781 tacaattcaa agcgtattgt ttttgccaga cgttttgtaa agcctcccac ctcacgcttt
     9841 gtacggcgta taaccacctg cgcaattttt tctttaagtg gtgcaagatc agcatctggc
     9901 acacagtagg ttgcctgaaa gctgtgtact ggccccaaca gatttttatc aagaagtgtt
     9961 agcagataat acagttcctc aagctttccc cgaaacggcg ttgccgatag caaaagcagc
    10021 gctttgctgt tttttgccag tatctctgcc acctgatatg attttgtctg agtggccgca
    10081 tcacggcgca ggcgatgtgc ctcatcaata acaaccgtat cccactgaac tttcgataac
    10141 gtttcaataa agctgcggtt tttaataaaa tcaatggagc agattatttt atttaaaacc
    10201 catggattgc ctttataccg cgtatatatt tttttatcaa caatggtaaa gtcttcatta
    10261 aacttgcttt tcatctcgtg ctgccactga tacagcaaac ttgcagggca cacaatacaa
    10321 accctgtcat aattatacct gtatgatagt tctttaataa taagccctgc ctcaatagtc
    10381 ttgccaagcc caacctcatc agcaattaaa aatcgcttgt gcagtgaatt gaccacagtg
    10441 aaggtgcttt caatctggtg gggaagtatc cgtgtacgtg aattagatag cgatagcatc
    10501 ttgttattga catacgcact tgaaagcaca aagctttcgg ttttaagcaa aaaacgctta
    10561 gtgggcacaa acgaatgttg cgctatataa tccagaatta cagcatcaga ggatgcagta
    10621 tcctgtacac tgctatattc ttcatctaag ggtagatgaa cttgttgcat acagattcaa
    10681 ttccttgtat atgtgatgta ttctttttat ggtataaagg atagtccaat gtgacataat
    10741 atacaaatag ctcgctatta caagcatttt tttaatatgt atcgtattac ccattcaatc
    10801 gtatggggaa aattttatgt tttatattac cgcatgtcag ctctgaaaat atcaatactg
    10861 taggggatat tttcatctaa gattggcata attggtgttg tacggggttt aaatccacgt
    10921 gccaggttaa cacgggcaaa ttcattttcc atgcccaggc gtgaagcata atcaattgct
    10981 ttccagtatg caatactgct ttttgtctca ctaccctgaa tctggtatat ggcaccaaga
    11041 ttattatatg ctgatgcaag tgactggaat atgatacggt gctctttgcg ctccggccgt
    11101 ggcaatcgta ttgactccgc cttgcgttca aatacggcaa tcattttcag gtattcccct
    11161 ttgcttgcct ctaaattatt catatgatag aacgcgttgc ctatcgcaaa cataacctga
    11221 ggattagtaa ctatatcctc atacagatta agccactgtt ccagcgcctg ctgatagttc
    11281 tggttaacat aatatattct gccaaggtta taccgaacct caggtgagga aaaattttcc
    11341 tgaatagcca gctcgtactt ctgccgcgca atatctaagt ttgccatttt atcagcatct
    11401 tcatcaagaa gcgcttcatc aagctcatcg ccaaacctaa attttacctt atcaaagaaa
    11461 taataaaaga tgtgacccag atatgcatac gctttgccta ttgactcaga ctcatagtaa
    11521 aaatcctcat aggtaaactc aggcgggtat tgataagcag ttattgctgc attgaggtat
    11581 ttataggctt caaccggctg tttaattgtg tagttgtatt cacccagaag ctgcaatgct
    11641 gctgcatacc gttccccttt ttcctcagct ttttgctcag cttcttttaa aagcatttcc
    11701 acaagcttca gatttttttg tgctgcagcc agcttggcgc ttgcaacata cagcggtgga
    11761 taatcagggt ctttttcttt caatgcatca agcacaccac ggcatgctgg caccaggtta
    11821 tcctcttcgt ccttcaggcg tggcgactgt atacctaaat caacacgtac attaaacgta
    11881 tccgaacggt ttttacccag atagtaatgg gccaattttg ccagcagtgg cgaagggata
    11941 tctgttaaaa tatcacgcgc acgcaggtca gtatggatgg atagcacttc gggcaaatta
    12001 tcacgctcaa taaaaagatt aagaagacca gcatagccaa cccatgaatt ggggtctact
    12061 ttcagaatgt tttcaaaata ctgccgtgat tttaaatact gcccctgata cagatatgca
    12121 ttgcctacgc caaacattgc ggtaatattt tttgggtcaa ttgcaagcac cctgttgtac
    12181 atcattattg ccatatcaac ctgtgacatc tcataggtgc ctttgggtgc aaatgtcctt
    12241 agtgcatcgt tttgtatgcg actataatag ttttgcggta tttttgcaaa gaaatagccc
    12301 gtgttattca gtgtgtcaat atcacgactg tttatttcaa atgctcgttt caacttgctg
    12361 tacgcatagt caaactctcg ttttttaaag tatgcgcgtg catatgcatt gtatccatat
    12421 agataatctt ttttataatg ctggtcaacg tattcaaaat attcttctgc ctttttataa
    12481 tcctttactt ttttggttgc agggtcaccc ggctccagaa taagtgccac acctttatta
    12541 ataagccttt ttgccatgaa aggcttataa ataaattgat agacagttac tgttaatgca
    12601 aatccaatga gtaccgctgc tgcaataatc tttgtttgcc gcagcaatcg ctgctgtcgc
    12661 tgcatccctt cacgggtata ttcaggcctg gcagtgacaa cccttcgatg tggaatttct
    12721 tcttcaaggg taagtttaat attgagtttc ttttcaataa acctttgtat attgtcttca
    12781 ggtttgccag acagtatcag gtcaataagc tttcgtgtat cttcaggaga taactcatcc
    12841 cgtaaaatta tctcttttat ctttctgcgg agaacgggat gaagcagcag cattgctttt
    12901 tttagccgcc gcaactcagc atcagaaatt tcaacgcttt cttccattga gattggctgt
    12961 ggctcttcct cttccaggcc ttcaaattca ggcttgaatt cttcaactac gctctcaacc
    13021 ttttcagctt ttcttaccgg ttcttcaaag ctttcaagtt catcaaatga aggagtttca
    13081 aaacgagccc gtggagtttc ttcttttgaa acttcctcaa atgctgatgg ttcagggatt
    13141 tcctcatcaa gtcctaatgt tgtaagatca gcttcaggaa gttcttcagc tttaatttca
    13201 gaaatttcag atacttcttc agtgccaatc tcagaaagat gtatatcctc atctaaactg
    13261 acatcacggg ggatacgtgg ttcctcagtt acaaattcat cggtacggat ttcatgggct
    13321 tcaacagttt cgggttcaaa ttttgtaagc tcttccagtt catccagtgg gcttacttct
    13381 ttctttggtg gctttggtgg cagttccttt tcttcaaacg aggggatttc ttctggcaac
    13441 tcttcaggca acgtttcttc aaaagctttt tcaggttcct gtggcaatgc ctcttctgaa
    13501 acgtgctctt caaattcagg aagcgcttct tcaactgaag gtggcatttc ctcaggcact
    13561 tcttctatca tgtcggttat atctactatc tcttcagact cttcatatac ttcttttgta
    13621 gttaagcctt ctccttcctc ttcacgttgc ccgggagtaa tggtaaaatc agcagctgat
    13681 gaatcagcct gtatcacgct taccctgcca cgcatctgag caatggatgc aagttcagca
    13741 tcaaatgaat cgactattcg cgtgatttca gcttcttctt ccggagttaa attttgtatt
    13801 tcttcggtta ccgccataat gggctctcgt tatctttgat tatcctgaat aatgtgacat
    13861 aaacatacta atcatattct gtttaaaaaa tcaaacatat tttgccaatt atatattaat
    13921 atgcattttg aataaaatca tataaatatt ttctctatac ctatcgacat attaatatgt
    13981 aatttataag cgtaaaattt agccacagga gcccatagta catgccaaaa ccgctgcaat
    14041 ggattatgct catcatttca ctgtgttatt tttccagtag tgattattct ccagcaaaaa
    14101 gcgttcctta ttattcactg tacacccttt caaaggctat gccaccttct ccagtaactg
    14161 tctcccatag taaaccggga atgccacaga tactacgggg gactttgatt acctataaaa
    14221 accgcagtgc tcaccgggta tatattgccg gtgatttttc atactggaaa aagcagccta
    14281 tggagcgaag taataatggc gtctggtttt acatcattcc ctttaatgat gatgatcgtg
    14341 tagtgcggta caaatttctg gttgatgatg tgtggattaa cgatccttta aatcatgata
    14401 ttgctgatga caatgcaggt tcatatgttt ccattgtgcg ccaggataaa ctgtctgaag
    14461 gcacacaggt aagttacaaa atacttgatg aaagtatacg gctggtggaa ttcaggctgt
    14521 atgcaccaaa agcatctatt gtggcacttg ccggcgattt caacaactgg aacccggaag
    14581 ttgatctgct gcaaaaagac aaaaatggaa tatggcgtct gcaaaagcga ctaccaaaag
    14641 gagcattccg ctacaatttt gttgtggatg gtatctggaa gctggattta tataatcctc
    14701 atactgcatc caatgcagca ggaatgctct gttcagttat acagtttcaa tgaatacaaa
    14761 tatggttttt attctggcga tagttactga aaaactataa tcctttaatg acagtagcaa
    14821 gtgctaagat aacaaagcct attgccacaa gccaccatga atatgcagtc aatacaggca
    14881 gagcaataaa cttgccgatg atgcctaata ctccaataac aactgaaatc caccatacaa
    14941 tggatcttgg cgcatttgtt ctcataataa acctcatata aaaattgatt tagtatgtat
    15001 tggttatata ttaattattc ttaattttgc caatgtcaag taattacttg tgtttttttg
    15061 taattcatca tgtacagatt cattaaagat tccatatgtt aactgaggat tgacaccaag
    15121 aagcgttata ccattataca cacacattca cataacgatt atgaaagtat gacataattc
    15181 aaagtgagat aacaaaaagc tcactttttt gtagcaacaa taatgtaaat ttttgcaata
    15241 taatcaattc atcagattta ttgttatacc gttttgcata tattatcaat gcagcacata
    15301 tcacagtgtg gttttttggt acatatatct tttccatgct tcacgatgag cgcatgatac
    15361 tcattataat catgcacggc gccttcaaag ttttgatgaa acattttctg tatagcagta
    15421 taatcctcat tgccggttaa aattcccaat cgcgtgaaga tgcgttttgt ataggaatct
    15481 accacaaata ttttccgctc cagtgcgtat aacagtattg aatcggcagt ttcaggtcct
    15541 atgcggagca gcgcaagtaa ttcttcacgc aacgtttcgg tgggtagctt ctgcaaaatc
    15601 tcaaaactga agtcatactg tttaaaccag cgtaaaaaag tagcaatggc tgctgctttc
    15661 tgattgtaat agcctgaaga ttttattaat ggagctatcg cctccggctt ttttttgaaa
    15721 agcttttggg gttgcaggag atcattcttt tttagatttt caattgcttt tgccgcattg
    15781 gtccatgaaa cattttgcgc cagtatagca cctatagtta cttcaaattg agtttccccc
    15841 ggccaccagt gttgcggacc atggtgtgat agtaactgtt gataaatttt gtttacaatg
    15901 tgcatgcatt aaaaacttac tggcaggcgt ttgccctcag gctgatcaac aaggatgtcg
    15961 ccatcttcct tatacttttt cagcatgaaa tgggtaaccg ttgattgaac atgatctatt
    16021 gttgcaagct tttcagcaac aaatgatgca acctcacgca gagatggacc ttctaccagt
    16081 accagtaaat catagccgcc agacagaaga tacacagaag taacctcagg gaaacggtag
    16141 ataatttcag caacagagtc aaaaccaacg ccgcgttgtg gtgtcacctt tacttctatg
    16201 agtgcacgca cttcatgctg ttttatacgc tgccagttaa tatgtgtttt atatcgtaaa
    16261 atgatgcggt ctttttccag tttctttata tacttttgta ctttgtcaac gcttacatta
    16321 agttgttttg ctatatcttc aggtgttgcc tttgcatcat tggataaaat ttccagaatg
    16381 tcttcaccta atttatcaat tgagtatggt ttcattgtat attcctccaa aataaaattc
    16441 ttctcaacta aactacattg tatattttta gtcaatataa tttacttgca taatttgtta
    16501 tgtgtataag tacaatacat caatttgtta ttgtgacaga tactatttcg ttcgtattaa
    16561 tagctaataa atagctaata ccatattttt atagttacat caacatgaaa aaaccatatc
    16621 tttttatcat accccatggt ggctacaaag tacctgatga actggctgag ttatgtatgc
    16681 taagccagct agagttattt tttgaagccg atacaggtgc caccgaatgc tttaaactga
    16741 aagaattagg taccgtgata agtactcctg tgcaccggct gtttattgat gttgaccggg
    16801 atccttatat gcttccacca aagtcaaaca atggtgttat taaaacatcc acctcacaaa
    16861 aaaagcaaat ttttccagcc gggatattcc ctgacgaaat tgccattgct ggcatgatta
    16921 agcgctactg gtttcctttc cacagcagaa ttgaacaggc actggggaca agccgtttta
    16981 ttgtggtttg ccacacagtt gcacctgttg caccacctgt tgctgctgat gctggaagcc
    17041 cgcgccccat catcacgctg gaacatatca ccagtaatgg gagtgatatt acaacctgtg
    17101 atgaaacatt gacggtaaag ctactgggga ttttacagaa ggcttttgcc ggtgaaaaat
    17161 atactgtcac cacaccgttt tgtattggca ggcctgcaaa gcgctacatt atgtacaaat
    17221 acgcaaaaca tttattgcca atcattcgga tttcactgtc aaaagcattg tttgtgaacg
    17281 aaaaatattt tgacccacaa accctcacca ttgaccctga gcgtattcag ggcatacaac
    17341 aaagactata tgagtgtttt gaagcgttta tacgttttat gtaagaataa ttttggaaaa
    17401 ggaattctta atacagtacc cataatattc aaaatttgcc actcgcggta gaaaacattt
    17461 aaataaaata aaaatttttt tcgtttgcag tggataaaat ttttgcttga aataataaac
    17521 atttttattt tatatttggg acagatagta tatatttatt atattatata tatcaatatt
    17581 cttggtattt atcctttaac ttttttcacg taaacaatgc ttgattaatg taattattct
    17641 aaaaactaat aatttcaaga ggaaaaccaa catgaatgca gtaaaatcaa tatataaaaa
    17701 tgccccaagc agtatccccg tacccaagga attccagaag aaaaacattg aggtaatttt
    17761 tttacctctt gatgaagaaa aaaaatccat tgatgtaagc aaattttttg gtgctatccc
    17821 cgattttcct gaacgcgcac cacagggcga actacaaaca cgagaaaact tctaatgaat
    17881 tatcttctag acactaacgt ctgtatacaa tttataacag gaagaagtat tgcactcaaa
    17941 gaaaaaatag aggctgctga tatatcttct ttatttatat gttctattgt gagaggtgaa
    18001 cttgaatacg gtgcaagaaa aagcaataat ccagaaaaaa ctttaacaat cttaaaagaa
    18061 ttccttgcat gttttcctga aattagcttc gattcagcag cagctgaatg ctatggcatt
    18121 atacgagctg aattagagaa gcagggaacc cctattggtc cctatgatat gcaaattgct
    18181 gctatagccc ttgcacataa ctttacactt gtaacgcaca atatcaaaga attccaacgc
    18241 atcaaaaact tacgtattga agattgggaa tagataagca ttaacaagcc tacaaagaac
    18301 ctgtacaact tcatcaccac tctttgttcg ttatatactc ataagcactg attgatgctg
    18361 tggcgccatc ggctgctgcc atcagcacct gccggtatga gccttcacgc aggtcacctg
    18421 ctgcaaaaag cccacctatt ggtgtacgca tgtgcatgtc aaccttcacc tgagcagctt
    18481 catttaaaac ctctttgggc agatatgcag tgttggggct aaaaccaata aatataaaaa
    18541 gcccattaac agtaagctct gatgttttgt ttgtcttttt attcaaaagg ctaattgcct
    18601 ttactttttc atcgccaata acctgtgtca ccacagtatc taaaaccagt tcaattttgg
    18661 gatttgagcg caccctatct tgtaaaatct tctgtcctct gaattcatca cggcgatgaa
    18721 taaggtacac ctttgatgca aagcgtgtta aatacagtgc ctcttccagt gcggtattac
    18781 cacctccaac aaccgcagtt accatatcct taaaaaaagc tgcatcgcag gtagcgcagt
    18841 atgaaacacc tctgccggta aattcacttt cgcccggaac acccaggcgt ttgagagatg
    18901 caccggttgc tgcaatcact gaacgcgctg taagaacgct accatctgtt gtttttattt
    18961 caaatacatc ctgtacttta cgtatctgtt ccacatcttt gctttctatg gttaccccta
    19021 acctgcgtgc ctgctgttcc atgttgcttg ccagttgcca gccttcaaca ggttcaacaa
    19081 agcctggata attttcaatt tcatacgtca gcataacctg tccgccaggg gatatttttt
    19141 ccagtaccag cgtatcaagc cctgcacgca tcgcatagat tgatgctgcc attcctgcag
    19201 gtccggcacc aataatgata agttctttca tgctgtaagt cctcccataa aaatagaatt
    19261 tcccgacgta agtcaagttc attccgggct tgacttataa tcctcgtaac gcttacaatc
    19321 tactgtaata gtctgtgatt ataattaaac cactgtattt taggtttttc taaaatacta
    19381 tcgtacacat caaacctttt ttcatttttt tcttttgaca ttttgtgcct ttacaaaaac
    19441 taatgtcaaa tactttatac tgtgctatat tgttagaaat cgttttacca caaaagatac
    19501 acatcattga tatcgtttta aaagttagca cattatcagg gactatcgcc tcataatact
    19561 atatgggcga tagttaccga tactaaatcc aatacgaaag agtgaggcta tggaagaaat
    19621 aatcaaatat gaaaatggca acatcgaagt acctgacaac cctgtagtac ttttcataga
    19681 aggcgatggc accggtcccg atatctggcg agcaacaaag attgtgctgg atgcagcagt
    19741 gaagaaagcc tatggtggca agcgcaccat acaatggcag gaaattcttg ctggcgaaaa
    19801 ggcattcaac atgactggaa gctggcttcc cgaagaaaca ctggaaaaaa taaaaaaata
    19861 taaagttgca atcaaaggtc ctcttaccac accagtaggt ggtggcatac gaagcattaa
    19921 tgttacattg cgccaggtac ttaaacttta tgcatgcgtt cggcccgtac gccatttagc
    19981 tggtgtgcct agcccgctga aaaatcctga aaaagttgat atggtgatat tcagagaaaa
    20041 catggaagat gtgtatgcgg gcattgaatg gaagcaggga actccagagg caaaaaagat
    20101 tattgatttt ttaaacaacg aaatggggca gtcattaagc tatgatacag gcattggtgt
    20161 aaaacccata agcatagaaa atacaaaaaa catagtgcgt gcagccatcc gctattgcat
    20221 tgataacaat cgtccaagtg tgacaatcat gcataaaggc aacattatga aatttaccga
    20281 aggtgcattc cgcgactggg cataccagac agcaaaagaa gaatttgcac gttacattat
    20341 taccgaagat gaactctggt ctacctataa cggaaaggta ccagcaggga agattgttat
    20401 aaaagaccgc attgctgatg cgctgtttca gatggttttg cttcgtcccg atgagtttga
    20461 tgtcattgtt actcctaact tgaatggcga ctatatttca gatgcactgg cagcacaggt
    20521 tggtggcctt ggtatggcac caggtgcaaa cattggcgat aatgcagcag tatttgaagc
    20581 tactcatggc actgcaccta aatatgcaaa tcaggacaag gttaatcccg gttctcttat
    20641 tctttcaggg gagatgatgc tgcgtttcat gggatggaag gaagctgctg atttaatcat
    20701 aaaaggctta gaaaaaacca tacaacagaa gcgcgtaacc tacgacctgg aacgacagat
    20761 ggaaggtgca actcttctta aatgttcaga atttggccag gcagttgccg ataacatgta
    20821 aattacacag gtgttttcca gatatttata gctggatcat cgttacactt tttacagaag
    20881 gctttggtat tgccatgccc acatccgtag caccagatat caaatacgcg gcattctttg
    20941 cataacgctt tgccacaccc atcacagcgg acaaccgctt cttttgcatg gcaaacctca
    21001 catagtacac ccgatacagt ttccatatac ttcccagcgc atagtatagt gatgctatcg
    21061 tttttctgga tagcctgtta tatcacgtat atcctgatac aaaggttgca acctgttgta
    21121 caatctttta taaactttct tatatagctt atcatagata gcaacatctt tgggattggg
    21181 gtagaacacc ttaccaaccc gtgtcatggc actaacagca gttttaaaat caggatacat
    21241 cttcaaaccc actgcagcat caattgcagc accaagtgct gatgtttcaa acgtatgtgg
    21301 cctctctgct ggaaggttaa aaatatcagc tgtcagctgc atggctacat cgctctgggc
    21361 acctccacct gaaacgcgca atttttcaat aggcaccttg ttgcgctttt gtgtgcgttc
    21421 tgcaccttca cgcagtgcat acgctaatcc ctcaagtatt gcacggtaaa tgtgcacacg
    21481 agtgtggaca tcaccaaatc caatgatagc accttttgcc tcaggaccag gaatcttaac
    21541 accgggtgac cagtatggct gcaatgtcag ccccattgaa ccaggtggta cttcctttac
    21601 cagcctgtca aacagtgctt caggttttat gcccatcttt ttagcctgaa gttcttcacg
    21661 tgcaccaaac tcctttacaa accagctcag catccagaag cctctgtaca ccattacctc
    21721 tgtattatag gatcctggta ctgcactggg atatggagga aagaacggaa tgatttcaac
    21781 atattgagta ttggttgttt ctaccgttgc agtggtgcca tagctcaaac aggctatgtt
    21841 tggtgttaaa cagcccgaac ctaacacctc acatgcttta tcagctgcac cggcaataag
    21901 aggcaaaccc tgtggtattc ccgtttcatt agaagctttt ttggttatat agcctaacac
    21961 ttcagtaggt ttcaccagcc gcaccagagt atccctgctc atgggaaaaa gcttccactt
    22021 taaatcagag gcaggtgccc aatcctgtgt tttataatcg aagggaataa atgcaacctg
    22081 gcagcccgtt gaatcaacaa aatcaccggt taatctgtat gtaaaaaatc ctgataacaa
    22141 taaaaactta tatgtctttt cccatatttc tggctggtac tgacgtatcc agtttgcttc
    22201 agcctccgtg ataaaatact ctaccgcatc aagcatattt acaagtctta atcccgcttt
    22261 tatataccat ggcaatttaa caatcgactt atcagcacgg cgctgatcaa gccacacaat
    22321 agcaggcctc agtggcttgc cgtttttatc cacattaatc accgtatcac gttgtgtggt
    22381 caacgtaacg ccccggatag cgtccttttt taccttccct tttgcaaaaa gtttttttgt
    22441 agtggtgcac aacgttttcc agtaatattc agggtcctgt tcagcccagc caggataatt
    22501 actgaaatac ggttctattg gagtttttac tatatcaaca acatttccct gtaaattaaa
    22561 gactattgct cttatggact gtgttccggc atcaattgat aaaataagtt ctgaattttt
    22621 tgccatagtt ttcacctgtt gtacaaataa ttattttgtg cattcctatc gcttttaaag
    22681 gatgtacaca ttattattca taatgtcgtt gtattacgta tagtgatatt ttatcaacat
    22741 aaaattataa gaatttgttc taaaaatcac tttttaagta ccaacgtttt cccctctgta
    22801 acaagttcat ctattttttg tttatggatc tcatcaagaa aactttgaaa ttcatcaact
    22861 atctcaagaa cccactgcca gtatatcttt atatcatcac tatcaaaaga aacaaaacct
    22921 ttgtaatcag catcctttct tcgcatgtaa aggttagtca ggtaattata tacattcttt
    22981 ttaaccgaac ctggtttaat aaaatgctgc gcaagtaaca cctgtattcc ttcatgggaa
    23041 attgggaaat aaccccgtgt tgcaagcaca tacctcacaa ggccttcagc tacgtgaaat
    23101 aatgctgtaa cacattcacg gtaattgtga gcattgtaat ttacaatgaa acctttcagt
    23161 ttactttcta taagagtaaa aatgtcatct aacccttgcg ttgtctcaac catacagtgt
    23221 cacctttttc aatttctttg ccaattgctg atgaatgcaa attgttagta tcaataacaa
    23281 gaaaatggac cagtatttcc ggtgaattat caatgttcca ttgctcaatt aatttttcca
    23341 cttttttgct attgtgtaaa ttatctatca caacagcaat atccacatca ctgtattcat
    23401 tacttacacc cttactacgg gaaccataca ctacaatact attgacatac ggaacactgg
    23461 taatatattc tacaaagtca ttaatcaatt tttcttcata tggcgtaagt ttactcattg
    23521 caacaatcat tcctgtttac ctgataaaac aagtatcacc ctgtctatgc aaagtcaaga
    23581 taataaaaat taatattgcc agatttgccc atgctatagt gtactgtatg taaatacatg
    23641 aggtgcagta tgtttacgct tattgagaca aaaccggtag cagacgatca ttatctgctg
    23701 acgttacatt gtgacgctaa agaaagtatc ccgggacaat ttattaacat aaaagtaaat
    23761 acatctactg atccgctcat acgccggcca ttttccattt ttgaccatga ccaaactacc
    23821 atacacattg tcatccgtgt ggttggcaaa ggcacagaaa ggatagttgc cctgcatcct
    23881 ggccctgtag attgtattgg accatttggc aaagggtttt cagtagttac tggcaaaaag
    23941 gctttactgg ttggtggtgg tgtgggaaat gcaccactgt attatttagc caaaatgcta
    24001 cgtagagtaa atactcaggt tacctatatc tatggttcgc gttcagcatc tcacattttt
    24061 ttgctagata ggtatcagga actatcgcac acatgtattg taacaacgga tgatggaagc
    24121 ttgggtatta agggcaatcc cattgatgtg gcaaaagaca ttgtggcaaa acacaagttt
    24181 gatattgtgt acacctgcgg ccctgaaatt atgatgaaac gcctttcccg ggaactatcg
    24241 cacataccca ttgaagcttc acttgaacga tactttggct gcggcgttgg cctttgctca
    24301 ggatgcgctg tggcaaccat caatggctac agacgcgcct gcattgatgg ccctgtgttt
    24361 gatgcccaca cagttctgtg ggatacccca taagctactt gaacaggggc gatagttgtt
    24421 tgaaacgttc tgtccctgcc ttttccagaa tcataaatga tattgtttcc gtgggataga
    24481 ctattgcacc tgcctgtctg agtgcatgta ttgccatttc ccattcgtga gtgcgtcgcg
    24541 aacacaccgc atctgatgca atgatgacat tataattttc tttaagcagt gacagagccg
    24601 tggtgagtac acatatatgt gtttcaatgc cgcacaaaat gatattggtg cgttttatcc
    24661 tggtaacctg ctctcgaatg gtattatctt tcatgcaatc aaaagccagc ttatcaaatg
    24721 caaccactcc ctccaggcga tccatcaact ccttgattgt gggtcccagt cccttgcggt
    24781 attgttcggt aaggattatg ggaaggctat actgcttggc cagctcaatg agaatgccta
    24841 cattctttgc cacttttttt cgcatctcat attccattga gttaaacaaa ctatcctgca
    24901 catcaataat aaacagtgca gtattgtcag gtgtaatata aaattttgtg atatccatag
    24961 taatcaccgt atcatattat ctatagaatc aaggatagta tcatcctttt tcttcatctt
    25021 ctttaatatc tgctctttct gtttttcgga tacattgata tgaggtattt ttgttgtggg
    25081 caatggagaa ccagttgcac atttagtaca ttcttcctgt ggctcagtcc ccgggatgaa
    25141 aagctcatca ataacctttt tgcaagaata tgatggcaaa agccctgatt ctgcacacac
    25201 tttcaccgtg gtgatattgc catatacccc aaagtccagt accggctcct tctgtaatgc
    25261 acagctcata tactgtcccc acactggtgc taccacagca ccacccgatt gtcccggacc
    25321 aagcgacaat cctttgccat catatcccat ccatataccg gtaacaagct gcggtgtnnn
    25381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaca ttaatagaca gcgccagcgc
    25501 acggcgtaat gtcaccaggc cgtaatattc gccttcatag ttttccggta tccagtcgcc
    25561 accctccagg tctaaataca gaatgggtga atccataatg gttgtggcag cggtgaattt
    25621 ctttgaatca attgccgcag tatataaaag cggctttatg gccgagcctg cctgccgttt
    25681 tgattgcatg acgcggttaa gctgattggt gctggtaaaa tcgcttccac caaccattgc
    25741 ctcaatgtag ccattggttg ggtttattga aatgaggcaa ccttccacat tcataaattc
    25801 tttctggtcc cagcttccct caaaatactg ctgcagtgca tcctcaaccg tgtcaagacc
    25861 tgcaatgagg tttaatgcct gcaattccca caataagcca tccttcatat accggttaaa
    25921 cttccgtttt tcagctgacc cactgctttt tattgctggc aaaccaaata gcatattcaa
    25981 taaatttact tcatcagcaa agcggttaga tacatactca tcattatcaa atgccagact
    26041 gcgtgaaatt accgtctgct tttcaacggc atctttcaaa agcctctgtg cacactgctg
    26101 tttatcaaca tccagtgtgg tgtacacggt aagaccatct tcatacactg ctttttcgcc
    26161 aaactttgca accagtatgc ggcgtatgtg ctcggtaaac catggtgctt tgttcacacg
    26221 cgatgaccat gttgtcatgg ttggtggcaa atcattaatg tatgctaaat aatccggcca
    26281 gaaatcaaga tatgcttttt cagcctgtgg tactgtgata aagcccatat caaccatctt
    26341 ggcaagtgca accctgtggc gttccataga aagacgaggg ttttttaggg gcgatagtac
    26401 attgggagca gatggcagtg tggcaagaag gctacattca gccagattaa ggtcccacac
    26461 atgcttacca aaataaaatt ttgctgctga ttcaacgcca tacgttccat gccccaaaaa
    26521 aatctggttt aagtacatct ccataatttc atctttggta taaaacaact caatcatgag
    26581 tgaaataaac gcttccttta ccttacggta tagtgaacgt tcaccagaag taagcaaaat
    26641 ctttgcaagc tgctgtgtaa tggtgctgcc accctgttta acgcgccctg aaacaaggtt
    26701 aacaaaaaaa gcccttacaa tgcctttggg gttaatgcca aaatgttcat aaaattcatt
    26761 gtcctccata gcaataaacg cctgcacaac ataaggagga attcttttaa ggggaacaac
    26821 ctcacgcttt tccctgaaaa attcagcaac cagtacatta tttttatcat aaatctttgt
    26881 agtctgatac ggtgtgtaat gggcaagcgc ccgtacttta taaaaatcta taaccagaat
    26941 aacggtaaat gctatgacag cacctgctat aatgccaatg atgataggcc gtacattgcc
    27001 aaaacgcaat tccagcgcat acaggatatc ctcaaaaaaa ttaatgatcc gcacaaaaaa
    27061 atcacctgca ttaatgtacg attttttgct aaattttcta tttacctgat atcgtcgcat
    27121 gatttacaca caatactgcc tgatggtcat ggtattaaca aagttaaacc ttacaattca
    27181 agtcaacaca ttattgcaac aattttttta ttttcaggtt actatcgccc accataaaca
    27241 ttttcttgac acaaaacagc atttcgtata catgtttaag gaaacaaatt aaaaacaaaa
    27301 agtaaaagag gcacctatga taccatttac aaagcttcat ggcattggta atgattatat
    27361 ttacataaac gcaataaaat acccggtaaa aaatccccgg aaactagcca ttgcaatgag
    27421 tgatcgccat tttggcgttg gttccgacgg gctgatcttg atattgccat cacaaaaggc
    27481 tgatttcaga atgcgcatgt ttaatgctga cggtagcgaa gcagagatgt gtggcaacgg
    27541 aatccgtgcg tttgccaagt atgtgtatga ccacaagtta actaagaaaa cacatatttc
    27601 ggtcgaaaca ttagctggta ttaaatattt gcaatgcacc gtaaaaaggg gcaaagtgca
    27661 tacagtaacc gtggatatgg gggaaccaat tttacttcgt gataaaatcc ccatgatagg
    27721 caaccctggc atggttattg aggaagaaat acacgttgat ggtgttaaat ttccaataac
    27781 tgctgtttca atgggcaacc cccatgccgt tatttacgtt gaagatgtca aaaattttcc
    27841 cgtagaaaaa tatggaccaa tgatagagaa ccatgaactg ttccctaacc gtaccaacgt
    27901 tgaatttgta gagatagtta atgaaaaaga agtaatacag cgtacatggg aacgcggtag
    27961 tggtgaaaca ttagcctgtg gaaccggagc atcagcagta actgtggcag gtgtacttac
    28021 caaaaaaaca tctcgtaaac ttaacgttca tctttccggt ggtgatttga caatagagtg
    28081 gaatgaaaag gataaccacg tgtatttaac aggcccggca gaagaagtgt ttgaaggcgt
    28141 gtggcctgaa taatatctac cacagattct tgccatatat accatgttcc agaagaacaa
    28201 ctctggtaat gcccggtata ttacgactta ttgtttgttc cagtgcctta ttaaatgctt
    28261 tgaatgaatc cggaatatag ggcacctgac cttctattgt acccagctca cagtctatga
    28321 tacaggtagt gtcataaaac catacatggc gcacaacaat atcaataggc gctatggcac
    28381 gggtattttc aaactctgat ccttcggcta caagcttaac aagccgtctt accttgcttt
    28441 cattattctg tggcagtaat acttgccgtg tttctttcag aatagatttt gcatctaaat
    28501 cggcaacaaa gatgtctatt gtttcacggg tatccagtgc aggcaacgat ggataaatat
    28561 caaatatatt tttgtcagta agaactgata tggcagcata atcaataata catagtatgc
    28621 tgactgctaa cagtaccatg cggtagcgct ttttggtatc tgctggcgga ttaactacaa
    28681 gttcaataaa agcagtaaag tagtgcctgc attgttctat taatgtatta tacaattgcc
    28741 aggtttttcc tttatacttt ttatatgcgg ttttcagttt gtcaaaagaa cgtttatggt
    28801 tcatacagta tcattccttc acagtgtgat atgggaaatt tattttctac aataccatta
    28861 cagtaatcac tatgcactgg taactatcgc acacattaat tattcaatac tgttttattg
    28921 tatgtattaa taaaacgcat aatgccttca gttataccac gtgcaagctt tttttgataa
    28981 tttttactgg tcagaaaacc tttttctttt gtattggtaa tatannnnnn nnnnnnnnnn
    29041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29101 nnnnnnnnnn nnnnnnnnnn nnnntcttca tttgaaggat tctgcgaaag ataataggtt
    29161 tcaaaacctg aaattttact cacaacagct gcattgcaat gtatgcttac aaatattcca
    29221 ttgacgcctt ttttaagcaa cctgtttgct atttcagtac gctcacccag ttctaaaaat
    29281 ttgtcggtgg ttcttgtcat taccacatca atacctggca attttttctg tagctctaat
    29341 gcaacctctc gcgcaatctg taatgttaaa agcttttctt taatgccgct tttaccaatt
    29401 gctccagggt cttttccacc atgtcctgca tccaccacaa taaatccaat agcatctttt
    29461 gatacgtgtg gtaatggctt tttttctttt tgtttttcct gtggtaccac tgcttccttt
    29521 ttgataacga tactgttgcc ctgtatggca acagcatacc cttccagcag gttttctatc
    29581 attggcagag caatatctaa aggaatcaaa acttctccct gcatgcgtgt aacaggatac
    29641 tcacttctaa gcagcatgtc acctgatagc gcaaccgaca tgccaaccgt atagattgca
    29701 aatcgtggac cacgcattat cttcccgcgc tgtagcagta tgtcaaatga cgattgtacc
    29761 ggcagcagtg ttcgaatatc atattcagat atataagcat tacctgaaac aatctttaca
    29821 ggtagcgcga tactatcctg cgaatatatg tcagaataaa caggtataaa ggctatattc
    29881 aaaagaagaa ttacacagcg ggtaatgcgc ttaacaatgt cacgggattt cagcattgat
    29941 ttgttgtaag gtactatcgc ttgcctgtaa agtaaaattt ggaatagata cgccagttac
    30001 ttattgatga aaccaccatg aatagtacaa ctaacagtaa tgatatttgc tgtaatgtgt
    30061 agccagcaaa cgtatgcaaa ggattaacaa tgaaaattaa accacaaagt cccatgataa
    30121 caaccataca tttccctgca atacttggcc gcacctgaat atcatgtgca taaaaaagat
    30181 aaaagcctgc aatgatatgg gtaatatcac ggatgaggat gaggacaatc atccacaatg
    30241 ggaattgcct gaaataataa agggtaacag ccacaatgag tatgacacat ttatctgcta
    30301 cagggtctaa aaactgtcct aaccgcgata cctgatgaag caatcgtgct accataccat
    30361 caataaaatc tgaaactgca acaacaagat acagcgccac tgcaacctta cagtaccaga
    30421 tatcccctgt catgcgttca tagtacaacg caacagcaat ggcaggcgcc aaaagtaccc
    30481 gtactactga tatcagattg gaccacgttg caacacgccc ttcaagcaaa tccttaattc
    30541 tcattaccgt taaccactca ttgcactgta ttccctcata taatcaagcc agaaccgtat
    30601 agcgtcaagc taaattcttt aaaaagcttt ctcattaaaa gtttattgac taaatacagt
    30661 gttttcttca tatatcatta catccaaatt atttttttgg gcgatagtta ctgtaacata
    30721 gttttaacc
//
LOCUS       DAORYL010000031        29992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1310_length_29992_cov_14.654241, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000031 DAORYL010000000
VERSION     DAORYL010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 29992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29992
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1310_length_29992_cov_14.654241"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..715
                     /locus_tag="PLV81_07460"
     CDS             149..715
                     /locus_tag="PLV81_07460"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOM09974.1"
                     /translation="MIESEVLVLNAGFVPIRITTVKEAICLLLSDKAVPVVEEDTFIR
                     SPSIAIKVPSVIAILNYHHLPRRKVVFSKLNVIYRDDMQCQYCGKRFQMKHLTVDHVI
                     PRSRWEVVTGKSLKEGFSSWHNLVCACKWCNNQKGNKLMHEIGWSLLREPFEPEYIPY
                     IIVTAEKAKKKGWLPFCGFNVKIVEMIP"
     gene            complement(712..2637)
                     /locus_tag="PLV81_07465"
     CDS             complement(712..2637)
                     /locus_tag="PLV81_07465"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007955153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOM09975.1"
                     /translation="MGYSVCYKILQAHLKEGKLEPGTEIGIVIDQTLTQDATGTMAYL
                     QFEAMGIDRVKTEISVSYVDHNTLQMGFENADDHKYLQTMNAQFGIYYSKAGNGICHQ
                     VHLERFAKPGKTLLGSDSHTPTAGGMGMVAIGAGGLDVAVAMGGGLFYLTCPKVINIY
                     LTGKLRPWVSAKDIILTVLQKLTTKGNVGYVIEYTGPGIATLSVPERATITNMGAELG
                     VTTSLFPSDKVTREFLVAQGRENDFVELLPDKDAQYDKVIEIDLSTIEPMIACPHSPD
                     NVVSVESIKGMKVDQVAIGSCTNSSYKDLVTVAKILKGKKIHPKVSCILAPGSKQVLH
                     MLAKGGFLADIIESGVRVMESACGFCIGNGGAPISGGVSLRTNNRNFEGRSGTKDAKV
                     YLVSPETAALSAIEGTLVNPLDMPAQSYPHIELPKTFIIDDSMIIPPFQSDEPIFRGP
                     NIGQPPKTQPLPQTIQAVVGIKVGDKITTDHIMPAGQRLKYRSNIPEYAKYVFEMVDS
                     SFSKRALDNKEKGLATVIVGGVSYGQGSSREHAAICPAYLGVKIVLAKSFERIHTANL
                     INFGILPLIFNSESDYDSIQVGDTVVLNDCIKQVESGNTITLLINNKPVECTLLATER
                     QRKILIAGGLLNYTTQH"
     gene            complement(2656..3537)
                     /locus_tag="PLV81_07470"
     CDS             complement(2656..3537)
                     /locus_tag="PLV81_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09976.1"
                     /translation="MDITIITDSGPHIGQGHWQRMLLFAWYCSHNGIPVTLVHKHDIA
                     APDGVSVRLSDSIPQNTCCILRDMRDSSKEEIEYLRAIAPVIVIDDCGEGRQYSTAAI
                     DLLPNIYGNTANTFESTVPLFLYGYNFYQSLSNAPGTIAKDIDCFIYASTFDAALLCE
                     ALGTHYSFVCTDSRTYTTNIALSSSMHYSNLIAQSKVVISHFGISIYEAMLCGCHCII
                     LNPTAYHDKLSHTLQNTNVLVHHQLYNHASVPQIMEIFKNFVEQAHTHVDTAHCIRTI
                     QTNLKNFYDALRLIITS"
     gene            complement(3539..4282)
                     /locus_tag="PLV81_07475"
     CDS             complement(3539..4282)
                     /locus_tag="PLV81_07475"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HOM09977.1"
                     /translation="MKNTYTITAIIQARMQSTRLPGKSVLPLAGKPLLYHVIERAKAI
                     QQVTQVVVAIPSDDDNNPLADIAELCNVNVFRGSLHNVLERYFFAYKQYGGNYIVRIT
                     ADNPFTDSHFASVAIEEELRTNADLCAVKNLPLGVGVEVIASTALDKAYMHSTKPYHH
                     EHVTPYIKENPDIFKIHYFDVDFANPFPSLRLTVDTQEDYRLASAIYDALYKGSVFTL
                     SDVIAFLEKNPHLVTINASIQQKPMTSHE"
     gene            complement(4299..5798)
                     /locus_tag="PLV81_07480"
     CDS             complement(4299..5798)
                     /locus_tag="PLV81_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spiro-SPASM protein"
                     /protein_id="HOM09978.1"
                     /translation="MNTQALIFVDSSINNDNPLTFNGQFIPDSIAQKIQSLPDIESIH
                     YCIPESYTGKLKQAASVFIYSNTDTPEMWQQIFTQTGALHIIKIPADAAFFQSSILSE
                     MLDTHIKYLSEFTFSYNLPSGYACEIISKSVLENPLANPTVQNSLCDIVKNNLNHFDV
                     ELYYKEPDLRWHRVNFRAANIRDRLVMQNIIAINNQTPAYELLQDIIDNHPEVLFTTP
                     SYVEIELCGHCNLDCIFCYRNILSPQHGTMELSTLQTLLEKMRIFNTPYAVCFGGSGE
                     PLQHPQFYKALELCNNEDLIHTIVIETNGIHADAGYASALASFTGSKVTTIVNINGHN
                     PETYKKLHNADYFNTVHNNILKLKEFLETNSKLNNNLYIQILKINETESFLDSYYDFW
                     EQHTIPIILQKQNTYIGRIPDKRYSDLTPLQRKPCWHLRQDLYILSDGTIAFCKQDID
                     GAAKRGNIITDDLEAMWKQQREHFVNDYKQNRAKKPNCSVCDEWYTYNL"
     gene            complement(5807..6748)
                     /locus_tag="PLV81_07485"
     CDS             complement(5807..6748)
                     /locus_tag="PLV81_07485"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOM09979.1"
                     /translation="MKKKLCIIIVTIVTAVPVFAWETFDRVIAVVNDTAIIQSELMRR
                     YQIVTKKQTPKKPEELNSILDNLIERALIEQEAREQAIIISDEKVMTHIHNIMKSQKI
                     PSLDVFKKMVEEKEGISFEEYKEEIRQSLLTEMLVSLAIGVSPPSRDEAYQWYKENKN
                     KLGNEVHIKQIVIRPKNSSFAEEKRANELINQIRDRILKGESFEKLASQYSEDPSAKN
                     GGDIGWIALAEMDPYMANTVFRMKKPGEISTVIKTQTGYTIVKLIDSRPTSFETVQDR
                     ILNMLYQRNMGEQFKKWIVTRRAQSDIKIYLENYRQS"
     gene            complement(6753..7547)
                     /gene="murI"
                     /locus_tag="PLV81_07490"
     CDS             complement(6753..7547)
                     /gene="murI"
                     /locus_tag="PLV81_07490"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOM09980.1"
                     /translation="MDSRPIGIFDSGIGGLTVCKAINKLLPGENLIYFGDTARFPYGT
                     RSAETLIRYARQITSYLLSRNVKMIVIACNTSSAAALPTLQQENTIPIIGVINAGARA
                     ACKRVHNNVIGVIGTRATVSSKSYEKAIHAINPAITVIQQHATMLVSLIEEGWIEDDV
                     TRLTIQRYLQHMIDQGVKTLILGCTHFPILKNTIHEVYPQLDLVDTGEEIAREVNDIL
                     TKKNLANNSSSGTIELYASDITDTMQNLKKLFFNGHDVPIEKLVIG"
     gene            complement(7573..9159)
                     /gene="cimA"
                     /locus_tag="PLV81_07495"
     CDS             complement(7573..9159)
                     /gene="cimA"
                     /locus_tag="PLV81_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOM09981.1"
                     /translation="MDTDKKHIILYDTTLRDGAQTFGISFSLQDKIRIALELDKLKID
                     YIEGGWPGSNPKDNIFFSEIRKANLKHSKIAAFGSTKRPKMKLTEDSLLQDLINSSAP
                     TLTIVAKSWDFHVTDALNISLEENLALVYDTIAYLKDKGFEVFLDAEHFYDGYKANPE
                     YAMKVLESAVKAGVDMLVLCDTNGGTLTHEISSITGTVCSTYKTPVGVHHHNDAGVAV
                     ANGIAAVLSGAISVQGTINGYGERCGNCDLTALIPNLVLKLGCSCNAAESLYHLTETS
                     RYISEIANLAHNERAPYVGDNAFAHKGGIHVSALKRNTSTYEHINPELVGNRRKVLIS
                     ELAGKSNIQVKAKELGISINNIELPEKILRRIKDLEDQGFQFEAAEGSFELLIRRSNG
                     EYAPFFTLKGFRVITEKDESGRVTCEATIKVEVDGQIEHTAANGNGPVQALDNALRKA
                     LENFYPEIRELQLSDYKVRVLNEKDGTGSSVRVLIDQKRHTEHWGTVGVSTNIIEASW
                     QALVDGIEYMLLKTRKNTSH"
     gene            9473..10606
                     /locus_tag="PLV81_07500"
     CDS             9473..10606
                     /locus_tag="PLV81_07500"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM09982.1"
                     /translation="MKQIRTYTYTQKQKKWITLLYIILSLLIITLPLVVYIFIYHVSR
                     QLISMPLYIFLFFLFIATLSIGIYTTIRFLISATREIVTSKNELALKDVIIQHSDEII
                     ILLDMFGRILSINPALTSILKFNKEDVIQHPFRVILFEKSFEDSIRLRDLLLSRFKDV
                     FKGYEAEIVLPCKVQHHSEIKSIAFKLIPVFQDNNLQFILAIGRVIQSDFLTSHYLKT
                     EYAEFIIDNNIRMVNLLSYRLTRNLEPYLEKREIIRIQLGLQEAIINAIEHGNLEIDF
                     ETKSRLKKMEGNYWDHVLDICNKDHLEKRKIKVQYHLFIDCVKYIITDEGKGFDWKQY
                     VRAAPDGNLIHTYHGVGLTMLQDIFEVSFNEKGNEITLVKRFS"
     gene            10795..11217
                     /locus_tag="PLV81_07505"
     CDS             10795..11217
                     /locus_tag="PLV81_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOM09983.1"
                     /translation="MAEYRNPVPTVDIIILLEDENGNIKNDEIILIKRKNPPYGWALP
                     GGFVDYGETLEQAAVREAKEETSLDVTLLKQFHTYSDPSRDPRQHTITTVYIAKAKGT
                     PHAQDDAKEIGIFNVNDLPQPIAFDHASIIKDFKNNVY"
     gene            11231..>12748
                     /locus_tag="PLV81_07510"
     CDS             11231..>12748
                     /locus_tag="PLV81_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOM09984.1"
                     /translation="MDVSYNLLNLTGKAIDILAKFSKADIRVHDVGRIPKAPVVFVVN
                     HFTRMETFFLPNVIHKITGKYIYSLAHHSFFVGGFGTFMEKVGAVSTKDPLRDVKFIG
                     ALLRGDMDCIIFPEGQMVKDKKLVERGKYMVYNSGIRRPPHTGAAFIALRAQFYREEL
                     KSCKERNACDELNNLMAHFGISEQELSHITNQETYIVPVNITYFPIRARANILNKIAN
                     KLVKALPERIEEELEVEGTMLVEGVDIDINFGNPIAISPFLKSYLAKQKIKSKKLYLD
                     PQELQSDVLLRKIAIDIMYRYMRDIYAMTTLNHDHIFSYFLTKTKNRIKETDFKTKAY
                     CAIESIKKVNLATCHTSLQLKQGYLLTDDPHGRYNDFIDAAKSEGLISIKDGFIYKNK
                     EKFSRLYEFHTIRKDNIVEVLKNEIEPLEQVIKKLDRVFWTPMFCIRRKLVKSFYKRD
                     REIFEKDYKEFFKQGETKPKHIGMPLFKKRWFAHTGIVLVHGYMAAPEEMRQLAEF"
     assembly_gap    12749..12848
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12849..13272
                     /locus_tag="PLV81_07515"
     CDS             <12849..13272
                     /locus_tag="PLV81_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOM09985.1"
                     /translation="ISAPYRLQNIAAGLAPVVDTWNKFLSFLKIKKIGRMDFIPNHPD
                     NPDINYLRNPVSGIHQMEVLMNLVEKRLADISIPVLIMQGAGDKTVDPKGAFEMFMKI
                     ASEKKEFTMVHSKSHGITRGEEGKVVARRVAAFIKDYA"
     gene            complement(13294..14562)
                     /locus_tag="PLV81_07520"
     CDS             complement(13294..14562)
                     /locus_tag="PLV81_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09986.1"
                     /translation="MKKLLILLGILSFSVHVSAQIHGTAMGGISTVTSEGAIDTTRNP
                     ALLGTISAATTSFYIMGNTYYNEDANPEFHTPVMDISSIEQSNDYYYAFSLFAGYARP
                     SGRGILGYSLSSKENFYLRKKDTQKIYGSIPNPTPPPTSLSFEQTETTTTSEINPTLS
                     IAYGWKLSDNNYAGIQLTTTPFLSNKKTDNNNSLGTSYSYTNQEYGVIIQPSFGLLLS
                     GNDSQVGLRFIPSTIKCIKKKAEADFSTTDLSYSAKWDFQQSEGPQIVAGGYAKILPQ
                     TGIALEFGLLLPSSYTNTDINVTDNPLPAINHSSVTIHNDTIINAKAGIQYSFTETFE
                     CMAGAAFFHFINTAGSSKSYGKGKFNLVLITFGLNYSLSSTVILSTLLLINNSSFESY
                     YHMEDTISLDAKTKTNLWNITVGAGLSYRL"
     gene            complement(14612..17011)
                     /gene="lon"
                     /locus_tag="PLV81_07525"
     CDS             complement(14612..17011)
                     /gene="lon"
                     /locus_tag="PLV81_07525"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOM09987.1"
                     /translation="MNFEEYFDNDTIQIENTILASDLSKIDTNLPSTIHIIPVRYRPI
                     FPGIITPLIIAKPRFMRSIDDALNTSRAIGLAVLKDDDVEDVEIDDIEMFGTAAKIIK
                     KINLPDESINVLINSICRFRITKIIDDKKSIIAKVEYIYDKANPKNLEIKALTRAILS
                     QLKILSESNPLFTEEMKLTMLNVDEPGRIADFVTSILNLEKQEYQNILETINVKKRLE
                     KVLVLLQKELEVVSVQRKIQNQINEKIYKQQRDYFLREQLKIIKQELGLDDDRSNEIQ
                     QMKSKIETLNLKGEPKEKIQDELNRLYYIESNTPEYIIARTYLDTVLSLPWNTYTEDC
                     LDITRAEKILKKSHYGLDDVKERILEFLSVRMLKPDSGGSILCLVGPPGVGKTSLGQA
                     IADSLKRTFFRVSLGGIRDEAEIKGHRRTYIGAMPGKIIQGLKICKSKNPVFMLDEID
                     KLYQSYQGDPAASLLEVLDYEQNHSFRDHYIDMPFDLSKVLFITTANTTDTIPDVLLD
                     RMEVIRIPGYIAEEKYKIAKHFLLPKQLKKHGLPKNSIELDRNAFFYIINGWAREAGV
                     RNLDRQLERLCRKKAKSIVKNDDSFPHLLDEESIRSLLGPEYYTGDTIPNKLKPGIAI
                     GLAWTSTGGSVLLVEALSTPAQGSSTLTLTGNIGDVMKESATIAYNYIKNYFSDDTQV
                     TDFFTKNTIHIHVPAGAIPKDGPSAGITMASALYSLVKHIPVTKRLAMTGELTLSGRV
                     LPVGGIKEKLIAAKREQCNTVIIPLENKKEMGDIPSHITRGLSLYFVAHVNEVFDIVF
                     K"
     gene            complement(17050..17691)
                     /locus_tag="PLV81_07530"
     CDS             complement(17050..17691)
                     /locus_tag="PLV81_07530"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOM09988.1"
                     /translation="MLDNLIQQLPVLLDSKSNPLLYLLLCISAILENLIPPVPGDVVT
                     AFGAFMVGTGKLHFFWVYLSTTLGSVIGFTLLFLVGKFFGKELLIKKNFTFFKKEAIL
                     KAEQWVQMYGIWLVLANRFLPGIRSVISLTAGFSNLSTYKVLLFSFISSSIWNLILIY
                     LGYSLGNNWLTVQGKVSHLLSQYSKVIVLLLLCLVIIYFFIKLKKYVAKKQLF"
     gene            18194..18721
                     /locus_tag="PLV81_07535"
     CDS             18194..18721
                     /locus_tag="PLV81_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09989.1"
                     /translation="MRKYILFIVVITMIACSTATVKEKEDPFAQDNKKFLEEKSGTEL
                     FRVLITPDNYQVVQLNYQGRITRNNDESGDKYILEEVAQYNQIDEAREGIVTVWLFPD
                     SGAIMKIRPTQLTFLVELDNLIVQDLQRWTFSFPEKTIEPTKFDVKYRIVLRKKLSDE
                     EIMQQIREKIKDKTQ"
     gene            complement(18728..21148)
                     /locus_tag="PLV81_07540"
     CDS             complement(18728..21148)
                     /locus_tag="PLV81_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09990.1"
                     /translation="MIWNIVTLVSCLFMILFFRRLDRSNLRITKVKRYTSKVLDDFKK
                     LAQEEERKFNDSTIELDIMLKKAQALSNHLNGSIAEIEEKLKGLDIEKTNLKKVEEDL
                     KVISNAARDVNQQIEYIAASRASFGDIAKKINYLTDNLSKLEKETSVVLQTFNDKVRE
                     RSRELSEEIAEQINKLRDSINIKEERILMASQEKVDLLTRNFSESLSRMEQNITNTGD
                     AILENIRLKIDTVTKSVDSIEQRVESAERRVFTDLNQKIATLAKSIEDFEQDLDQIRI
                     TSTSSMRKELAEVSDQILELKSSITEVENSIFADLKEKANDVKSSIASSMKEFKDMRE
                     TLLEQVNGDIEKVYDKLRLIEQNIDESKAQLIASFDEEVNKIRTAFDTLNLHAISKKD
                     EIVKAARKEAEDIKTRIEEFGEKFIEMEHRLVDKSEEQLKTLVTEYQAIELRFQNLTE
                     RANQIERQVHQSIESKIAEARNEFDTMSQHIASMKEELLSLEENHKIFTRTDDMLKTV
                     QGALTELNTILAQSREESKKLDEFLEKAESIKDIRRTVERELKVFESRKEKLATVESE
                     IKALMELADMISLKADSLQDGMLKIDQFNARIDALAKVYNDLENRIHELQEYETTITK
                     NLESVNKADLIMKSIETRIKAFQSTVEKSEKKIERLTGYLHSIEENTLILKTREQEIR
                     EVKEHFAELESLTAHIEERIKQVNAMFQKLEDMRDEISQTDSRLQLMFTETDKRMKQF
                     ADFLKAIESNTVIGKEIKGIPNVNINERLIKTVRELSNKGWSSQEIANKLLIDENAVR
                     LIINTSSL"
     gene            21487..21990
                     /locus_tag="PLV81_07545"
     CDS             21487..21990
                     /locus_tag="PLV81_07545"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HOM09991.1"
                     /translation="MIVSTIVVCAVALLAFAYQDLKPGHNLLPELSHTKKVKYKTTWK
                     ITAYCPGRCCNTRFTKTGIDDYTNCTAVGGLTLTGLLDTGVHVVAVDPSVIPLGSIIK
                     YNNTYYVALDTGSAIKGFTLDILMAEHRQAHDFGVQYCDDVEIFIPENPGEIIEAIKV
                     KAGVRKP"
     gene            complement(21977..23668)
                     /locus_tag="PLV81_07550"
     CDS             complement(21977..23668)
                     /locus_tag="PLV81_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOM09992.1"
                     /translation="MDGFSIGITIFGGLGLFLFGMKIMSESLQQAAGDRLKGILWKVS
                     NNRIKGVFTGLFITSIIQSSSATTVMLVSFVSAGLLTLQQSIGIIFGANIGTTVTGWL
                     VALIGFKVKITAVALPCIGIGFFIRFVSNEKVRYYGEVLLGFGVLFFGLDIMSNAVKD
                     LRNSEAILNMMTRFSAVDIPSTLIVVAIGTIVTMIVQSSSATVAMTMTLAVNGLIDFP
                     TACAFILGENIGTTITANIAAIGASTEAKQAARAHFLFNALGVLWIMLIFHKFFVPFV
                     DWIVPGNPYSDILAVRSRVIADHMAAFHTCFNVINTIVFLPFANILAKLSIKLAPKPE
                     DEKDEFHLKYITTALVSTPTININQARLEIKRMCGIVLEMFDMVIDVFHHPKEKLGTQ
                     VEQILKLENLTDHLEKEISNFLVNILQNNISYKQSEEVSSLLHTVNELERMGDNCESL
                     LKLIRRKYESGIEFSEEAVNGIDEIASKVREFLVLLNNNITIRNKDIMTDSKFLEDRI
                     DELRSELRKGHVNRLNEGVCDVNAGLIFIDMLSKFEKIGDHAFNVAESISGVRVF"
     gene            complement(23725..26679)
                     /locus_tag="PLV81_07555"
     CDS             complement(23725..26679)
                     /locus_tag="PLV81_07555"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM09993.1"
                     /translation="MNIKEFNYSSITQRRFVLLSVFFSIATLVVFTLLNILYVSSLTL
                     YSPVWKYTLTFAFSGLLAGIIAGNSILKRLQNHRIIFIIQEFIAVCVAIAPVIAAFVY
                     NDILNAYGTILQHCYWLLYGAIFLLYFSTGIKAPYYLKTTIGDYIDDKNPAILAVCFA
                     LLSFIASLAFGLFIFYNQHYLILYVVLPILSCVFTILLSLPFCPPAIISRGVAEERAI
                     TSRDDILFTYCNVSFISIFIILILLSIVSRYGLYLQIIFYSAIAIAAGFIVGTIASQY
                     IKTHNTVFIYGEMLYPFLFLGFIIIIYSNDIPYYISIMTALPFSIFSGLTFGTSLKQI
                     ILKNNHQSRYTILLFSFFIIPIPIIIAFLWIELTYLWFFILIYLMAIITIIFPGMYLL
                     QNTKRSVPSILYFVVSLLFIPAIIITHIYFRLPFSNTLVNRFCTGYDTVAMANFNASY
                     ITQPATAYIQGIPACVVTDHFARDMKRATVLSGLFAGSGHTIIIDGYHKFFTNNLYHI
                     LPAPVIIDYIPERIVDYQKPTITGVQQYATINSDILRGLSDKKGTIIDIPNIFDHYMY
                     AFHFTDNYIASIKPHVDDYYFTIIDITHIAPEQLHSLGKALQKSFTHSMVAIYGNLMI
                     IGATDKQDGFAIDQAKMQTLENLIQTTEVSNLFINPEHALSYILSTSVQSVLPDDGEN
                     STVMLKFSKPDATIYPNSFIDTVINTNDAVTGLADKTPYWANTLQLRMFYAKRINQLL
                     KQSEYYGLQEQYEREMQALMELRRTGEYTPSLRQYIINVIAYKEKLYENIALMYEKDK
                     QWDKAISLYSALLAVNPNNFTANYRIGIISATIQNMDNAFKYLNNAMRMNPNNPDVLY
                     QLGILLVSQGKYTEALNYLLQAHQMNMATASLFFNIGLCYESLKNIEEAKRYYEKAKL
                     LDPNDPSIQSSLERFSGKTEEPVNPNADTRTNQLDEEKGESFPLPINQSAYDVRLKEN
                     EVKEQDKLAMPQ"
     gene            27010..27330
                     /locus_tag="PLV81_07560"
     CDS             27010..27330
                     /locus_tag="PLV81_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM09994.1"
                     /translation="MHSKNAFEYIPEDNLVVLFVLMVCTAGLYYFWWIARVSRIFYDN
                     PVTNILLTILTAGVWLIYLTIKYMQKSEMLNGRQMPWYMVLFLPIGFLIIQHNINERY
                     FTSQ"
     gene            27348..29057
                     /locus_tag="PLV81_07565"
     CDS             27348..29057
                     /locus_tag="PLV81_07565"
                     /inference="COORDINATES: protein motif:HMM:NF025691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HOM09995.1"
                     /translation="MIVRCSECNAAYQVDDEKVDNKRFAFTCPKCKAHVIIDNRIDIA
                     ADKSIDDMIMAHAVEEKPKKMAQAPEEFDTLLQEEPFEKEYTAEKISTTDEDLLADIE
                     SFDATQDKKDKSKDEFEPIESETIDLSDFSAEELKSATEEITLPEEPIEKDLLLDEEI
                     KADEIFSKEKDVDESITIDLDTLDIPLDESTVIEEAEMPGESSVLEKEELLVEDMGFD
                     DILVADEEIPQKPKAKKAPEKEPIDDITLDLDSLDIQLEEHAEVLPGEQIIEEDEKLT
                     LEDAGITIDELIEEEKKQSLEEEEDLKLSLDEIEPGLTVDDLHKELTESEIETLMSKE
                     IELPEVDLDKFELEEGEIKEFAPEEFEYAEKESYDSIPAGFVNLTIDYALSYSRPGAV
                     ARLLGLYLVTLIPHIIVSSIYSLVSSIVGFFNWCVVLFTGQFIDDFADMQQNTLRYYI
                     AILASAAQITEDRPVYAGRPKVEHSLQLDIVYPSEPSRLLAFLRITGIGILTAALPHL
                     LLLAVLTLGMLILIPVGLISIIATKRWPSLLFDFMYRYLRYYSRVNAYIMGLVDKYPS
                     FIF"
     gene            29115..>29992
                     /locus_tag="PLV81_07570"
     CDS             29115..>29992
                     /locus_tag="PLV81_07570"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOM09996.1"
                     /translation="MDIHSIIHTVFSSGMLANYIHDFEYRAIQEQMATAIARSWYDEE
                     HIVIEAGTGTGKTFGYLLPAALFALLEDVKVAIVTETKALQQQLIVKDLPVVASILKN
                     ITDSSLEYALCLGSANYPCFRRLQHAIDTGSFDKKFAGEIANLQKLLKKNHTLTRFDV
                     QVPDALWNTICRESELCSMNKCRYKSTCSYMEARKQWQKAHILVMNHYLFFSNIAASK
                     SLVPDCKAVVFDEAHSLERIASGQLGFSINSQHLKETLEHMIQRKKGASLVEKWLPHG
                     LQQDAIEVFDNALRQV"
BASE COUNT         8959 a         5393 c         5929 g         9611 t
ORIGIN      
        1 actttttact tcatttttaa tgtgaaaaaa aataatattt tttcactaaa tattgttgac
       61 ataccttgta caacagctat tattatgcaa attttatatg cagggttttt attacctgta
      121 acgcttgtgt gcgtacaggt ggtacaccat gattgaatct gaggtactgg tactaaatgc
      181 agggtttgtt cccatccgta taacaacggt aaaagaggca atatgcctgt tgctttctga
      241 taaggctgtg cccgtcgttg aagaagatac ttttatccgc tccccatcaa tagcaattaa
      301 agtcccttct gttattgcta tccttaacta ccatcatctg ccgcgccgca aagtagtatt
      361 ttcaaaactc aatgttattt atcgggatga tatgcaatgt caatattgtg gcaaacgctt
      421 tcagatgaaa caccttacgg ttgaccatgt tatcccacgt agcaggtggg aagtggtaac
      481 aggaaaatca ttaaaagaag gattttcaag ctggcataat ctagtgtgtg catgcaaatg
      541 gtgcaacaat caaaaaggca acaagcttat gcatgagata ggctggagct tactccgtga
      601 gccatttgaa cccgaatata tcccctatat catcgttact gctgaaaaag caaaaaagaa
      661 ggggtggctc cccttctgtg gattcaatgt caagattgtt gaaatgatcc cttaatgctg
      721 tgtggtataa ttaagcaatc cgccagcaat taatatcttt cgctgtcgtt cagttgctaa
      781 caatgtacat tctacaggtt tattattaat gagtagtgtt atagtatttc cactctcaac
      841 ctgcttgata caatcattca gtacaacagt atcccctacc tgtatgctgt cgtagtcgct
      901 ttcactgtta aatatcagtg gcaaaatgcc aaagttaatc aggttagcgg tatgaatgcg
      961 ctcaaacgat tttgccagta caatctttac tcccagatat gcagggcaaa tagctgcatg
     1021 ttcgcgactt gagccctgtc catagcttac accaccaaca atgactgttg ccagtccctt
     1081 ttctttgtta tccagtgcgc gctttgaaaa tgaactatcg accatttcaa acacatattt
     1141 tgcatactca ggaatattag agcgatattt caagcgctgt cctgcaggca tgatatggtc
     1201 agtggttatc ttgtcgccaa cctttatacc caccacggct tgaatggttt gtggcaatgg
     1261 ttgtgttttt ggaggttggc caatgtttgg acctctgaat ataggttcat ctgactgaaa
     1321 tggtggaatg atcattgaat catctattat aaatgttttg ggcaattcta tgtggggata
     1381 gctttgtgca ggcatatcca gaggatttac aagcgttcct tcaatggccg atagcgcagc
     1441 ggtttcaggg cttactaaat aaacctttgc atctttagtg cctgaacgcc cttcaaagtt
     1501 gcgattgttg gttctcagtg acacgccacc agagatgggt gcgccgccat taccaataca
     1561 aaaaccgcag gcagattcca taacacgcac accactttca atgatgtcag ccaggaaccc
     1621 tccctttgcc agcatgtgca acacctgctt ggagccaggt gctaatatac aactgacttt
     1681 gggatgtatc ttttttcctt taagaatttt tgcaacagtg actaaatctt tgtacgatga
     1741 gttggtgcaa ctgcctatgg caacctgatc caccttcatt cctttaattg attcaacaga
     1801 tacaacatta tcaggtgagt gcgggcatgc aatcattggt tctattgttg acaggtcaat
     1861 ttctataact ttatcgtact gtgcatcttt atcaggaaga agctcaacaa aatcattttc
     1921 tctgccctga gctactaaaa actcacgggt aaccttatca ctggggaaaa gcgatgtggt
     1981 tacgcctaac tcggcaccca tattggtgat ggttgcacgc tcaggcactg aaagcgttgc
     2041 aatgcctggt cctgtgtatt caatgacata accaacattc ccctttgtag taagtttttg
     2101 aagcactgtc aggataatat cctttgcgct tacccatggg cgcagtttgc cggttagata
     2161 aatattaata acctttgggc aggttaaata aaaaagcccc ccgcccatgg caactgcaac
     2221 atctaatccg ccagcaccaa tggctaccat gcccatgcca ccagctgtgg gtgtgtggct
     2281 gtcggaacca agcaatgttt ttcctggctt tgcaaaccgt tctaagtgca cctgatgaca
     2341 tatgccattg cccgcttttg aataatagat tccgaattga gcattcatgg tctgcaggta
     2401 tttgtggtca tcagcatttt caaatcccat ctgcaatgtg ttgtggtcaa cgtacgacac
     2461 ggaaatctca gtttttacac ggtcaattcc cattgcttca aactgcaagt atgccatggt
     2521 gcccgttgcg tcctgagtca atgtctggtc aatgacaatg ccaatttcgg ttcctggctc
     2581 aagttttcct tctttaagat gtgcctgcaa tattttataa caaaccgaat atcccattgg
     2641 tacaacctct tgcgtttatg atgtgataat taatcgtagc gcatcgtaaa agtttttcag
     2701 atttgtctgg attgtgcgta tgcagtgggc agtatcaaca tgcgtgtgtg cctgttcaac
     2761 aaaattttta aatatttcca taatttgtgg aactgaagcg tgattataaa gctggtggtg
     2821 tacaagcacg ttcgtgtttt gtaaagtgtg cgatagttta tcgtgatagg ctgttggatt
     2881 gagaataata caatggcaac cacacaacat tgcttcgtat atagatatac caaagtggga
     2941 tataactact ttactttgtg caatgagatt tgaataatgc atactggatg atagcgctat
     3001 gtttgttgtg tacgtcctac tatcagtgca tacaaagcta tagtgtgtgc ccaatgcttc
     3061 acacaaaagt gctgcatcaa atgtggatgc atatataaaa caatcaatgt cttttgcaat
     3121 agtgcctgga gcgttcgata gtgactggta aaagttatat ccgtatagaa aaagcggcac
     3181 tgtggattca aaggtattgg cagtgttgcc gtatatattt ggcaataggt caatagcagc
     3241 ggttgaatac tgtcggcctt ctccgcagtc atcgatgact ataacaggag ctatcgcccg
     3301 taaatattca atttcttctt ttgatgaatc ccgcatgtcc cgtagtatgc aacaggtgtt
     3361 ttgagggata ctatcgctca accttactga aacgccatca ggggctgcta tgtcgtgttt
     3421 gtgcacaagc gtaacgggta tgccattgtg tgagcagtac catgcaaaga gtagcatgcg
     3481 ctgccagtgt ccctggccta tgtgtggtcc tgaatcggtt atgattgtta tgtccatatt
     3541 attcatgcga tgtcattggt ttctgctgta tggatgcgtt tattgtaacc agatgcggat
     3601 ttttttcaag gaatgcaatg acatcgctca gtgtaaaaac gctaccttta tacagcgcgt
     3661 catagattgc tgatgctaat ctataatcct cttgtgtgtc cactgtcaga cgcagtgatg
     3721 gaaaaggatt ggcaaaatct acatcaaaat aatggatttt gaatatatcg ggattttctt
     3781 ttatatacgg tgttacgtgc tcatgatgat atggctttgt actgtgcata tatgctttat
     3841 caagagcagt ggaagcaatg acttccacac caacaccaag tggcaaattt tttactgcgc
     3901 aaagatctgc gtttgtgcgt agttcttctt caatagcaac cgatgcgaaa tggctgtcgg
     3961 tgaaaggatt gtcggcagtg atgcgcacaa tatagttacc accatactgt ttatacgcaa
     4021 aaaagtagcg ttcaagcaca ttgtgtaaag aaccgcggaa tacgttaaca ttgcaaagtt
     4081 cagcaatatc agctaatggg ttgttgtcat catcactggg gatagcaacc accacctgtg
     4141 tgacctgttg tattgctttt gcgcgctcaa taacatggta tagcaggggt ttgcctgcaa
     4201 gtggtaaaac cgatttacct ggcagccttg ttgattgcat gcgtgcctga ataattgctg
     4261 tgatggtgta agtatttttc attgtgcggt actacatgtt acagattata tgtataccat
     4321 tcatcgcata cagaacagtt aggttttttt gcacggtttt gtttatagtc attaacaaaa
     4381 tgctcacgtt gctgtttcca catagcttcc aggtcatcag ttatgatgtt gccgcgtttt
     4441 gctgctccat ctatatcctg tttgcagaat gcaatcgtgc cgtcgcttaa aatatataaa
     4501 tcctgtcgca ggtgccagca tggttttcgc tgcagtggtg ttaagtctga atagcgttta
     4561 tcaggaatac gtcctatgta tgtattttgc ttttgcagta tgatgggtat ggtgtgctgt
     4621 tcccagaaat cgtagtaact atctaaaaaa ctttcagttt catttatttt cagtatttga
     4681 atataaagat tattgttgag tttgctgttt gtctcaagaa attctttcag tttgagtata
     4741 ttgttatgaa cagtgttaaa ataatcagca ttgtgcagct ttttgtaggt ttcgggatta
     4801 tggccattaa tattgactat ggtggttact ttactgcccg taaaagaagc cagtgctgat
     4861 gcatatccgg catcggcatg tatgccgttt gtttcaataa caatggtatg tatcaaatct
     4921 tcgttgttgc aaagttccag tgccttatag aactggggat gctgtagtgg ctcaccactt
     4981 cctccaaaac atactgcata aggggtgttg aatattcgca tcttttcaag aagtgtttgc
     5041 agtgtggata gttccatagt gccatgctgg ggtgatagta tattccgata gcagaatatg
     5101 caatcaaggt tacagtgccc acacagttca atttctacat aggaaggtgt ggtaaaaaga
     5161 acttctgggt gattgtcaat gatatcctgc aacaattcgt atgcaggtgt ctggttattg
     5221 atggcaataa tattctgcat gacaagcctg tcgcggatgt tagcagccct gaagtttact
     5281 ctatgccagc gcaaatcggg ttctttatag tacagttcca catcaaaatg gttaaggttg
     5341 ttttttacaa tgtcgcacag tgagttttgt accgttgggt ttgcaagcgg attttcaaga
     5401 actgattttg atataatctc acatgcatac cctgaaggca ggttgtaaga aaacgtaaat
     5461 tctgaaagat atttaatgtg agtatcaagc atttcagata gtattgaaga ctgaaagaac
     5521 gctgcatcag caggtatctt tatgatgtgg agtgcacctg tctgtgtaaa tatctgctgc
     5581 cacatttcag gggtgtcagt gtttgaataa ataaacactg aagcagcctg cttgagtttt
     5641 cccgtgtatg actcagggat acagtaatgg atggattcaa tgtcaggcag tgattgtatt
     5701 ttctgtgcaa tactatcagg tataaactgc ccgttgaatg ttaaaggatt gtcattgtta
     5761 attgagctat cgacaaaaat taaggcttgc gtgttcacgg tatttcctag ctctgcctgt
     5821 aattttcaag atatatttta atatcggatt gcgccctgcg ggttacaatc cattttttaa
     5881 actgttcgcc catattgcgc tgatatagca tattcaaaat tctgtcctga acagtctcaa
     5941 atgatgttgg tcgggaatct attagtttta cgattgtata gcctgtttgc gtttttataa
     6001 cagttgatat ttcaccaggt tttttcatcc tgaatacagt gtttgccata tacggatcca
     6061 tctcagctaa ggcaatccag ccaatatcac cgccattctt tgcacttgga tcttcagaat
     6121 attgtgaagc aagtttttca aatgattcgc cttttaatat acggtcgcgt atctgattta
     6181 tcagttcatt ggcacgcttt tcctcggcaa aacttgaatt ttttggccgt atgactatct
     6241 gcttgatatg cacttcattc cccagtttat ttttattttc tttataccac tggtacgctt
     6301 catctcgtga tggcggtgaa actcctattg ccagtgacac cagcatttca gtaagcaggc
     6361 tctggcgaat ttcttcttta tattcctcaa aactgattcc ttctttttcc tcaaccatct
     6421 ttttaaaaac atccagagaa ggaatcttct gcgatttcat aatgttatgt atgtgtgtca
     6481 ttactttttc atcgctaatg attattgcct gctcacgggc ttcctgctca atgagtgctc
     6541 tttcaatcag gttatcaagg atggaattta gctcttcagg ttttttgggt gtttgtttct
     6601 ttgtaactat ctgataacga cgcataagct cgctctggat tattgccgta tcattaacaa
     6661 cggcaattac tctgtcaaaa gtttcccagg caaaaacagg tacggctgta acgatggtta
     6721 caattataat gcatagtttt tttttcattg agctatccta taacgagttt ttcaatagga
     6781 acatcatgcc cattgaagaa taattttttt aaattttgca tggtatcagt aatgtccgat
     6841 gcatagagct ctattgtgcc tgatgatgaa ttatttgcaa gatttttttt agttaaaatg
     6901 tcatttactt ctctggcgat ttcttcccct gtatcaacta aatctagctg gggatacact
     6961 tcatgaatgg tgtttttaag tattgggaaa tgggtacagc caagaatgag ggtttttaca
     7021 ccctggtcaa tcatatgctg cagataccgt tgtatggtta acctggtaac atcatcttca
     7081 atccaaccct cctcaataag ggataccagc atggttgcat gttgctgaat tactgttata
     7141 gcgggattta ttgcatgaat tgccttttca tacgatttgc tgcttacagt tgcacgggtt
     7201 ccaataacac caatcacatt gttgtgcacg cgtttacatg ctgctcgggc acctgcatta
     7261 ataacgccaa taataggaat agtattctcc tgctgcagag tagggagggc tgctgctgat
     7321 gatgtgttgc atgctatcac aatcatttta acattgcgtg atagcaggta tgaagtaatc
     7381 tgtcgtgcat accgaataag ggtttctgca gagcgtgtac cataggggaa acgggctgta
     7441 tcaccaaaat agataagatt ttctccgggc aaaagcttat taatagcttt acatacagta
     7501 agccccccaa taccagagtc aaatatgcca atgggtcgtg aatccataac agttgattat
     7561 gcctgttgta tattaatgtg atgtgttctt tcgagttttc agtaacatat attctatacc
     7621 atcaaccaga gcctgccatg aagcttcaat aatattggta gagactccta cggtgcccca
     7681 gtgttcggta tggcgtttct ggtcaataag gacgcggacc gaagaaccgg tgccatcttt
     7741 ttcattgagt acacgtacct tatagtcaga aagctgtaat tcacggattt ctggataaaa
     7801 gttttccagt gccttgcgca atgcgttatc cagagcctga acagggccgt tcccatttgc
     7861 agcagtatgc tcaatttgac catctacttc tacttttatg gtagcttcac aggttaccct
     7921 gcctgattca tccttttcag taattaccct gaaacccttg agcgtaaaaa atggtgcata
     7981 ttcgccattg cttcgcctga ttaataattc aaacgatcct tctgctgctt caaactgaaa
     8041 tccctggtct tctaagtctt tgatgcgtcg tagtattttt tcgggcaatt caatgttatt
     8101 aatagaaata cctagttctt tagcttttac ctggatgtta cttttacctg ccagctctga
     8161 aataagaacc tttcgcctgt tgcctacaag ttcagggttt atatgttcat aagtggatgt
     8221 attgcgctta agtgctgata catggatacc gcctttatga gcaaatgcat tatcacccac
     8281 gtatggagcg cgttcattat gtgctaaatt tgcaatttca ctgatataac ggctagtttc
     8341 agttaaatgg tacaatgatt cagcagcatt gcaactacaa cctaatttta atacaagatt
     8401 ggggattaag gcagtcaggt cgcagttgcc acagcgttca ccatacccgt tgatggttcc
     8461 ctgtactgaa atggcaccgc ttaacacggc tgctatgcca tttgccacag ccacccctgc
     8521 atcattgtgg tggtgaacgc caacaggagt tttgtatgta gaacatacag ttcctgttat
     8581 agagctgatt tcatgggtca gtgtgccgcc attggtgtcg cacagtacaa gcatatcaac
     8641 gcctgctttt acagcacttt ccagaacttt catggcgtat tcagggttag ctttataacc
     8701 atcataaaag tgttcggcat ccaggaatac ttcaaagcct ttatccttta aatatgcaat
     8761 ggtatcatat acaagagcta aattctcttc aaggcttatg ttaagggcat cagtaacgtg
     8821 aaaatcccag gattttgcaa caatagtcaa tgtaggagcg ctggaattaa taaggtcctg
     8881 taacagagag tcctcagtta atttcatttt tggacgtttt gttgatccaa aagcagcaat
     8941 ttttgaatgt ttcaggttgg ctttacggat ctctgaaaaa aatatattgt cttttggatt
     9001 agatcctggc caaccgcctt caatataatc tattttaagc ttatctaatt ccagtgcaat
     9061 ccgtatttta tcctgaaggg aaaaagatat cccaaatgtt tgtgcaccat ctcgtaatgt
     9121 ggtgtcatag agtataatgt gttttttgtc agtatccata tatagctata aaaaattatg
     9181 atatatagta tgaagataaa tatattacat tttacactat actacaaata ttattaaata
     9241 attttatcat aagtgttata tttttttgca ttttatcatg aagcataggg taaagcattc
     9301 aaataatagt atactattta ttcaagctat tttttttcta ttaaaaacaa tttgcttgta
     9361 ttttataata aatattcact atactgctat tagggttact ataacaggtt ttgttacctt
     9421 ttatgaggat tccaaagtga ttattttacc ataagtattg ctggcgttag atatgaaaca
     9481 gatcagaaca tatacctata ctcagaaaca gaaaaaatgg attacgttat tatatattat
     9541 attatcgctt ttgataatta ctcttccact ggttgtctat atatttattt accatgtcag
     9601 cagacaatta atttcaatgc cattatatat ttttttattt tttctattta ttgccacatt
     9661 aagtataggt atttatacaa ccatacggtt tttaatttct gctaccaggg agatagtaac
     9721 cagcaaaaat gaattagcat taaaagatgt cattatccag cattcagatg agatcataat
     9781 cctgctggat atgtttggta ggatattaag cattaatcct gcattaacgt caattttgaa
     9841 gtttaataaa gaagacgtta ttcagcatcc attcagagtt atattatttg aaaaatcatt
     9901 tgaggactca atacggttac gtgacctgtt gttaagccgg ttcaaagatg tctttaaagg
     9961 atatgaagct gaaatagtct tgccatgtaa ggttcaacac cattctgaaa ttaaatcaat
    10021 agcatttaaa ttaattccgg tatttcagga taacaacttg cagtttattc tggcaattgg
    10081 gagagttata cagtcagatt ttctcaccag tcattatctg aaaactgaat atgcagaatt
    10141 tattatcgat aacaacatac gaatggttaa cctgctatca tatcgactta cacgtaactt
    10201 agagccatat ctggaaaagc gtgaaattat ccgtattcag cttggattgc aggaagccat
    10261 tattaatgca atagagcatg gcaatttaga gatagatttt gaaacaaaaa gcagattgaa
    10321 aaaaatggag ggcaactact gggatcatgt acttgatata tgcaataaag atcatctaga
    10381 aaaaagaaaa attaaagtgc aatatcatct gtttatcgat tgcgtaaaat atatcattac
    10441 tgatgaggga aaaggttttg actggaaaca atatgttaga gctgctcctg atggcaattt
    10501 aatacatact tatcatggtg ttggattgac catgttgcag gatatttttg aagtatcttt
    10561 taatgaaaag ggcaatgaaa taacactggt aaagcgtttt tcataactaa tcttatttga
    10621 agtaattcag gttgaactca cttttttaaa tatgacgata tatttcatta cttcaaggtc
    10681 actgagtgat acgacaagcg gccactgagc tacgtcgaag tgtggccact gagctgagtc
    10741 gaaggattac tctgtatggt tttttagcgc taaatttttt aagaggagat gcccatggct
    10801 gaataccgta atccggtacc aacagtagat ataataatat tactggaaga tgaaaatgga
    10861 aatataaaga atgatgaaat tatcctgata aaaagaaaaa acccaccgta tggctgggca
    10921 ttgccaggtg gctttgttga ttatggtgaa acgttagaac aggcagcagt acgtgaagca
    10981 aaagaagaaa cttcattgga tgtaacatta ttaaaacaat ttcatacata ttcggatcca
    11041 tcacgggatc cgcgacagca tacaatcacg acagtatata ttgcaaaagc aaaaggcaca
    11101 ccacatgcac aggatgatgc taaagaaatt gggatattta atgttaatga tctcccgcaa
    11161 ccaattgcat ttgaccatgc atccataatt aaagatttta aaaataatgt atattgaaaa
    11221 ggtacaagcc atggatgtat catacaatct attaaacctg acaggcaagg caattgatat
    11281 actggcaaag ttcagtaagg ctgatatacg ggtacatgat gttggtagaa ttccaaaagc
    11341 tcctgttgta tttgtggtaa atcatttcac ccgtatggaa acattctttt tacccaatgt
    11401 gattcataaa attacaggga aatatattta ttcacttgct catcatagct tctttgttgg
    11461 tggatttggt acattcatgg aaaaggtggg ggcagtgtca actaaagacc ctctgcggga
    11521 tgtaaagttt attggagcat tgcttcgtgg tgatatggat tgcatcatat tccccgaagg
    11581 tcagatggtt aaggataaaa aactcgtaga gcgtgggaaa tatatggtgt acaattcggg
    11641 gatacgaagg cccccgcata ccggagcagc gtttattgca ttacgtgcac aattttacag
    11701 ggaagagctg aaaagttgca aagagcgcaa tgcctgtgat gagcttaaca acctgatggc
    11761 ccattttgga atatctgaac aggaactatc gcacataaca aaccaggaaa cgtatatagt
    11821 gcctgttaac attacctatt tcccaatcag agctcgtgcc aatatactta ataaaattgc
    11881 gaacaaactg gttaaagctc taccggagcg tattgaagaa gaacttgaag ttgaaggaac
    11941 aatgcttgtt gaaggagtag atattgatat taattttggt aatcccattg caataagccc
    12001 atttttaaaa agctatcttg caaaacaaaa aattaaaagt aagaaattgt acctggatcc
    12061 tcaggagttg caatctgatg tgttgcttag aaaaatagct attgatatca tgtacaggta
    12121 tatgcgtgat atttatgcta tgacaaccct taatcatgat catatatttt catatttttt
    12181 aacaaaaaca aagaacagaa tcaaagaaac tgattttaaa acgaaagcgt attgtgcgat
    12241 agaatctata aaaaaagtga atctggctac atgtcacact tcactacagc tgaagcaagg
    12301 gtatctgtta accgatgacc cacatggcag atacaatgat tttatagatg cagcaaaatc
    12361 tgaaggattg atttcaatca aggatgggtt tatatacaaa aataaggaaa agttttccag
    12421 attgtatgaa tttcacacca tacgaaaaga taacattgtt gaagttttaa aaaacgagat
    12481 tgagccattg gagcaggtaa ttaaaaagct tgatagagtt ttctggaccc caatgttttg
    12541 tattagaaga aagcttgtta aaagttttta taaaagagat cgagagatat ttgaaaaaga
    12601 ctataaggaa ttcttcaaac agggtgaaac taaaccaaag catatcggga tgccattatt
    12661 taaaaaaaga tggtttgctc acaccggcat tgtgcttgtt catggatata tggcagcacc
    12721 tgaagagatg cgacagttag ctgaatttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12841 nnnnnnnnaa tcagtgcacc ttaccgatta cagaatattg ctgcaggact tgcaccagta
    12901 gtggatacct ggaataaatt tctttcattt ttaaaaataa aaaaaattgg cagaatggat
    12961 tttatcccca atcatccgga taatcctgat attaactatc tcaggaatcc tgtttcaggt
    13021 attcatcaga tggaagtatt aatgaacctg gttgaaaagc ggctggctga tatatcaata
    13081 ccggtgttga ttatgcaggg tgcaggggat aaaacggttg atccaaaggg tgcgtttgaa
    13141 atgtttatga agattgcctc ggaaaaaaag gaatttacca tggtgcattc aaaaagccat
    13201 gggattaccc gaggtgaaga aggcaaagtg gtggcacgga gagtggctgc atttattaaa
    13261 gattatgcgt gagcatgaca tacatgcaga aacttacaat ctatatgata gacccgcacc
    13321 aactgttata ttccataaat tagtttttgt ttttgcatcc agggatatgg tatcttccat
    13381 gtggtaatag gactcaaaag aagaattgtt aattaaaagc aatgttgaaa gtataaccgt
    13441 agaggataac gagtaattta atccaaaggt tattaatact aaattgaatt tgccttttcc
    13501 ataacttttg gaacttcctg cggtattgat aaaatggaag aatgcagcac ctgccataca
    13561 ttcaaatgtt tctgtaaatg aatactgtat accagcttta gcattaataa ttgtatcatt
    13621 atggatggtt actgatgaat ggtttattgc tggtagtggg ttatccgtta cgtttatatc
    13681 tgtgttggta taggaagaag ggagcaataa tccaaattca agcgcaatgc cagtttgtgg
    13741 cagtattttt gcataaccac ctgcaactat ctgtggcccc tcagactgct gaaaatccca
    13801 tttggcgcta tatgaaagat cagttgtgct gaagtcagct tctgctttct tttttataca
    13861 ttttatagtt gaaggaataa atcgtaatcc cacctgcgaa tcattacctg atagtaacaa
    13921 tccaaaagaa ggctgaatga taacaccata ttcctggttt gtgtaggagt aactggttcc
    13981 taaagaatta ttattatcag tttttttatt tgaaagaaat ggtgttgttg tcagttgaat
    14041 acctgcatag ttattgtctg aaagtttcca gccataagca atactcaatg tagggtttat
    14101 ttcactggtt gtggttgttt ctgtttgttc aaatgataat gatgtgggag gaggtgtggg
    14161 atttggaatg ctgccatata ttttctgagt atctttcttt cgtaaataaa aattttcctt
    14221 tgaggacaga ctgtagccca gtatacctct acctgatggt ctggcatacc ctgcaaaaag
    14281 tgaaaaggcg taatagtaat catttgactg ttcaattgaa gaaatatcca taacgggtgt
    14341 atgaaattca gggtttgcat cttcgttata atatgtattc cccatgatgt agaatgatgt
    14401 tgttgctgct gatattgttc caagcaaagc agggtttcgt gttgtatcaa tagctccttc
    14461 ggaagtgact gtggatatac cccccattgc cgtaccatga atctgggcac tgacatgtac
    14521 agaaaaactt aatataccta ataatatcaa taatttcttc acagaccgcc ccatcttctt
    14581 gtatattaca caaagtaacg agtaaaataa gttacttaaa tactatatca aacacttcgt
    14641 taacatgggc aacaaaatat agtgacaacc cccgggtaat gtgcgatggt atatcaccca
    14701 tttctttctt attttccaga ggtataatca ctgtattgca ttgttcacgt tttgctgcaa
    14761 taagcttttc tttaattccg cccacaggta ataccctgcc tgaaagcgtc aactcaccgg
    14821 tcatggctaa acgttttgta accggtatat gctttacaag tgaataaagc gctgatgcca
    14881 ttgttattcc agctgaagga ccatcttttg gtatggctcc tgcagggaca tgaatatgga
    14941 tagtattctt ggtgaaaaag tcagttacct gggtgtcatc actaaaataa tttttaatat
    15001 aattataagc gatagttgct gattccttca tcacatcgcc aatattacct gtgagtgtca
    15061 aagtggaaga accctgtgct ggtgtggaaa gtgcctcaac cagaagaact gaaccacctg
    15121 tcgatgtcca ggcaagtcct attgcaatac caggttttag tttattgggt atggtgtccc
    15181 cggtataata ctccggtcca agtaatgagc gtattgattc ttcgtcaaga agatgaggga
    15241 atgagtcatc atttttaaca attgattttg ccttttttct gcagaggcgt tccagctgtc
    15301 tatccagatt tctaacccct gcctcacgtg cccagccatt tataatgtag aagaaagcat
    15361 tcctgtccag ttcaatggaa ttttttggca aaccatgctt tttaagctgt ttgggtaaaa
    15421 gaaaatgttt tgcaattttg tatttttctt cagcaatata gccgggaatg cgtataactt
    15481 ccatgcggtc aagtaaaaca tcgggtatgg tgtcggtagt attggcagtg gtaatgaaaa
    15541 gtacttttga aaggtcaaat ggcatatcaa tgtaatgatc cctgaatgaa tggttttgtt
    15601 cataatcaag gacttccaga agcgaagctg caggatcccc ctgataactc tgatacagtt
    15661 tatcaatttc atcaagcatg aacacaggat ttttagattt gcatatcttc aagccctgga
    15721 ttatttttcc aggcattgcc ccaatatagg tacggcgatg accttttatt tcggcttcgt
    15781 cacgtatgcc acccaacgat accctgaaga atgtacgttt taaggaatca gctatagcct
    15841 gtcccagtga tgttttccca actcccggag ggccaacaag acatagtatt gagcctccgg
    15901 aatcaggttt tagcatcctg accgatagaa actccaatat gcgttctttg acatcatcaa
    15961 gcccgtaatg gcttttcttt aagatttttt ccgcccgtgt aatatcaagg caatcttctg
    16021 tatacgtatt ccatggcagg gatagaacag tatccagata ggtgcgggca atgatatatt
    16081 cgggtgtatt tgattcaata taataaagac ggttaagctc atcctgtatt ttttcttttg
    16141 gctcaccctt gagattcagc gtttcaattt ttgatttcat ctgctgaatt tcgttgctac
    16201 ggtcatcatc aagccctaat tcctgtttga ttatctttag ctgctcacgt aaaaaataat
    16261 cccgttgctg tttgtatatt ttttcgttta tctggttctg tattttacgc tgaacagaaa
    16321 ctacttccag ctccttttgc aaaagtacca gtaccttttc aaggcgcttt tttacattaa
    16381 ttgtctccag aatattctgg tattcctgtt tttctaaatt taatattgat gtaacaaagt
    16441 cagctattcg gccgggctca tcaacattca gcatggtaag cttcatctct tctgtaaata
    16501 gagggttgct ttcagaaaga attttaagct ggctcaaaat agcccgggtt aatgccttga
    16561 tttcaaggtt ttttggattt gctttgtcat agatgtattc aacttttgca atgatacttt
    16621 ttttatcatc gataatttta gtgattctga accgacagat actatttatc aggacattaa
    16681 tgctttcatc aggaagatta atctttttga ttatctttgc tgcggtacca aacatctcta
    16741 tgtcatctat ctccacgtct tcaacatcat catctttcaa tacagctaaa ccaatagccc
    16801 gtgaggtatt tagtgcatca tcaattgaac gcatgaaccg tggttttgca atgataagcg
    16861 gagttatgat acctggaaat atgggcctgt atcgtacagg tatgatgtgt atggtagaag
    16921 gtagattggt atcaatcttt gatagatcgc tggcaagtat tgtattttct atctggatgg
    16981 tgtcattatc aaagtattct tcaaaattca ttaattatct ccacagaacc ataagtaatt
    17041 ttaatactat cagaataatt gttttttggc tacatatttt tttaatttaa tgaaaaagta
    17101 gattatcaca agacataata aaagcagcac tattactttg ctatattgtg ataacaggtg
    17161 gctaacttta ccctgtactg taagccagtt gttccccaat gaatatccta agtatataag
    17221 aataagattc catatagatg aactgataaa tgaaaacaac agaactttat atgtggataa
    17281 atttgaaaag cctgcagtca aagatataac actgcgaata ccgggtaaaa accggttggc
    17341 taaaaccagc catattccgt acatctgtac ccattgctca gcttttaata tagcttcttt
    17401 tttgaagaaa gtaaagtttt ttttgatgag tagctctttg ccaaaaaact taccaaccag
    17461 aaaaagaagt gtaaatccta taaccgaacc aagcgttgtt gacaggtaaa cccagaaaaa
    17521 atgtaattta cctgttccaa ccatgaaagc accaaacgca gtaacaacat caccgggtac
    17581 tggcggaatc aggttttcca gtatagcaga aatgcacaga agtagataca gtaacggatt
    17641 gcttttacta tctaataaca ctggcagctg ttgtataagg ttatctaaca taatgataca
    17701 ttctttggta aatttcagga attatagatg tacttactgt aaaaaataat ttacctgtta
    17761 ttattgaaac ttttatattg atactaagtg cattgattat ggtatattat gtaacattgt
    17821 gactgattgt tgcaaataat ataaacaggt attgaaaaaa attgatttac ttgttataat
    17881 gttattgttc aagtggatag aaaatctttc ataaagacga cactataatg ttctcattat
    17941 tttgtcaagt atgcttatac agtatatatt tttacattga agtaggttaa aaacaataat
    18001 aggtggttgg tgagctggtc atatttatta tgtgcaaatc caattgtatt aaattattga
    18061 caatgttata ttgatatacg attttaatat attgttctga gttgaaagca aagaatcaat
    18121 agttacaaac aacgatattc ttcatgtacg tagctttaca ctgaattctg ttttaaagga
    18181 gatgttgatg tttatgagga aatatatact atttattgtg gtaataacca tgatagcatg
    18241 cagtacagca actgttaaag aaaaggaaga tccatttgca caggataata aaaagtttct
    18301 tgaagaaaaa agtggtacgg agcttttcag ggtattaata actcccgata actatcaggt
    18361 agttcagttg aattatcagg gtagaatcac acgcaataat gatgaaagtg gcgataagta
    18421 catacttgaa gaagtggcac agtataatca aattgatgaa gcacgcgagg gtatagtgac
    18481 agtttggtta ttccctgatt caggtgcaat aatgaagata cgaccaactc aacttacatt
    18541 tctggttgaa ctggataacc ttattgttca ggatctgcag cgatggactt tttcatttcc
    18601 cgagaaaaca attgaaccaa caaaatttga tgtaaaatac aggatagtgc tcagaaaaaa
    18661 actttctgat gaagaaataa tgcagcaaat tagggaaaaa ataaaagata aaacccaata
    18721 aaaaacctta cagagaagaa gtatttatta tcagccgcac tgcattctca tcaattaaaa
    18781 gcttgttggc tatttcctga gaactccatc ctttattgga gagctctcga acggttttta
    18841 tcagcctctc attgatattg acgtttggga tgcctttaat ctctttgcca ataactgtgt
    18901 tactttctat agctttaaga aaatcagcaa actgtttcat gcgcttatcg gtttcagtaa
    18961 acattagttg taggcgacta tcggtctggc tgatttcatc acgcatatct tctaatttct
    19021 ggaacatggc atttacctgt ttgatgcgct cttctatgtg tgcagtgagg ctttcaagtt
    19081 cggcaaaatg ctccttaact tctctaattt cctgctcacg tgttttaaga ataagtgtat
    19141 tttcttcaat actatgcagg tatcctgtga gacgctcaat cttcttttct gatttttcaa
    19201 ctgttgactg aaatgctttt attcttgttt caattgattt cataataagg tcagctttat
    19261 tgaccgattc taaatttttg gttatggtag tttcatattc ctgtaattcg tgaattctgt
    19321 tttccagatc attatacact tttgcaagag catcaatacg ggcattgaac tggtcaattt
    19381 ttaacatacc gtcctgtaat gaatctgcct ttaatgaaat catatcagcc agctccatga
    19441 gagctttaat ttcagattca actgtagcaa gtttttcttt tcgtgattca aataccttca
    19501 gctcacgctc aacagtcctg cgaatatcct tgattgattc agctttttcc agaaattcat
    19561 ctaatttttt tgattcttcc cgtgactgag caagtatggt gttgagctcg gtcaaggcgc
    19621 cctgaactgt tttgagcata tcatcggtgc gcgtaaaaat tttgtggttt tcttccagac
    19681 ttaaaagctc ttctttcatt gatgcaatat gctggctcat ggtatcaaat tcatttctgg
    19741 cttcagctat ctttgactca attgattgat gtacctgccg ttctatctgg tttgcccgtt
    19801 cggttaagtt ctggaaacgc aattctatgg cctgatattc ggtaactaac gttttaagct
    19861 gttcttctga tttatcaacc agcctgtgtt ccatctcaat aaatttttcg ccaaattcct
    19921 caatacgggt ttttatatct tcagcttctt tacgtgcagc tttcacgatt tcatcttttt
    19981 tagagattgc atgaaggttt aatgtatcaa atgcggttcg gattttgttg acttcttcgt
    20041 caaatgaggc aatgagttga gcttttgatt catctatatt ttgttcaatt agtcgaagtt
    20101 tatcatatac tttttcaata tcaccgttga cctgttcaag taaggtttca cgcatatcct
    20161 taaattcctt catggaactg gcaatgctac tttttacgtc attagccttt tccttgaggt
    20221 cagcaaaaat actgttttca acttcagtaa tggaagattt aagttccaaa atctgatcgc
    20281 tgacttcagc aagctctttt cgcattgacg atgttgaggt tatcctgatc tgatcaaggt
    20341 cctgttcaaa gtcttcaatg gatttagcca gtgttgcaat tttctggttt aagtcagtaa
    20401 aaacacggcg ttcagctgat tcaacacgct gttcaattga gtcaaccgat tttgttaccg
    20461 tatctatctt taagcgtata ttttccagga ttgcatcgcc ggtattggtt atgttttgtt
    20521 ccatgcgcga tagcgactcc gaaaaattgc gggtaagaag gtcaaccttt tcctgtgaag
    20581 ccataagtat ccgttcttct tttatattaa tagaatcacg gagtttattg atctgttcag
    20641 caatttcttc agatagttcc cgtgatcgtt ctcggacttt gtcattgaag gtttgcaata
    20701 ccaccgatgt ttctttttct aatttggaaa ggttgtcggt aagatagtta atctttttag
    20761 ctatatcgcc aaaacttgcc cgggatgcgg caatatattc tatctgctga ttgacatcac
    20821 gtgctgcatt tgagatgact ttcagatcct cttctacttt tttaaggttt gttttttcaa
    20881 tatcaaggcc tttaagcttt tcttcaattt ccgcaattga accattgaga tgattgctga
    20941 gtgcctgagc tttttttagc atgatatcca gttcaatagt agaatcattg aacttacgtt
    21001 cttcttcctg ggctaacttt ttaaagtcat ccagcacttt tgatgtatat cgtttaactt
    21061 tggttattcg taagttggag cggtcaagac gccggaagaa gagtatcata aataaacagg
    21121 ataccagcgt cacaatattc catatcattg tttaatactc cttttatatg aaaattaact
    21181 gcaaatacac tacaattatg aaaaactata ttcccggtac aaagatttac ttttagcccc
    21241 tgcacggtat actatagaga acaaaaaatc acacttttta cattaaaatt atacataaaa
    21301 caattaatta aatttgtcaa cataatttta tgtcacatta aaaaatgatt gacttcactt
    21361 aaaaaaatta gaatttggcc aaaacattca attataaacc cttcactgtc tctggtggtg
    21421 aaggttttaa tacttttatt tgattttgaa aaacttacat agattggtca tacgatgatg
    21481 agaagagtga ttgtaagtac aatagtagta tgtgctgtag cattgcttgc ttttgcatat
    21541 caggatttaa agcctgggca taatctttta ccggagctat cgcacacaaa aaaagtgaag
    21601 tataaaacta catggaaaat aactgcttac tgtccgggtc gatgctgtaa tacacgattt
    21661 acaaaaaccg ggattgacga ctataccaac tgtactgctg tgggtgggtt gacattaaca
    21721 ggtcttctgg atacaggagt tcatgttgta gcagttgacc cgtctgttat tcctctgggt
    21781 tcaattataa agtataataa tacatattat gttgctcttg atacgggcag tgccataaaa
    21841 ggttttacgc ttgatatatt gatggccgaa cacaggcaag ctcatgattt tggtgttcaa
    21901 tactgtgatg atgtggagat ttttatccct gaaaatcccg gagagattat tgaagcaatt
    21961 aaggttaaag ctggggtcag aaaaccctga ccccggagat gctttcagcc acattgaatg
    22021 cgtggtcccc gattttttca aatttagata gcatatctat aaaaataagt ccagcgttta
    22081 catcgcacac tccttcattt aaacggttta cgtgaccttt gcgcaattca ctgcgaagct
    22141 cgtcaatcct gtcttcaagg aattttgaat ctgtcataat atctttattg cggatggtga
    22201 tgttgttgtt cagtaaaaca agaaactcgc gaacttttga ggcaatctca tcaatgccat
    22261 tgactgcttc ttctgagaat tcaattcccg actcatattt tctgcggata agtttcagca
    22321 atgattcaca gttatcgccc attcgctcca attcgttgac agtatgcagt aacgatgaga
    22381 cttcttcact ctgcttatat gatatattgt tctgtaagat gtttaccaga aaattggata
    22441 tttctttttc aagatggtca gtaagatttt caagtttcaa tatttgctca acctgtgtac
    22501 ccaatttttc ctttggatga tggaaaacat ctatgaccat gtcaaacatc tcaaggacaa
    22561 ttccgcacat acgtttaatt tcaagacgtg cctggtttat attaattgta ggtgtggaaa
    22621 ctaaggcagt ggtgatatat ttcaaatgaa attcatcctt ttcatcttct ggttttggtg
    22681 ctaacttaat ggaaagtttg gctaaaatat tggcaaaagg caggaataca atggtattga
    22741 taacattaaa acaggtatga aatgctgcca tgtgatctgc tattactctg gaacgtaccg
    22801 ctaatatatc agaataaggg ttgccaggta ctatccagtc aacaaagggt acaaagaatt
    22861 tatgaaaaat aagcataatc cacagtaccc ctaatgcgtt aaataaaaag tgtgctcgtg
    22921 cagcctgctt tgcttcggtt gatgcaccta tagcggcaat gttggcagtg attgttgttc
    22981 caatattttc acctaatata aatgcgcagg cagtgggaaa atcaatgagg ccattaactg
    23041 ccagtgtcat ggtcatggca accgtggcac tggatgattg tactatcatg gttactatag
    23101 taccgatagc gaccacaatt aatgtagaag gtatatcaac tgcactgaag cgcgtcatca
    23161 tattcagtat tgcttcggaa ttgcgcaggt ctttaacggc attactcata atatcaagac
    23221 caaagaaaag cacaccaaaa ccaagtaata cttcaccgta gtaacgtact ttttcattgg
    23281 atacaaaccg gataaaaaag ccaatgccaa tgcagggtag ggcaacagcg gtaattttta
    23341 ctttaaatcc aatcaatgca acaagccagc cggtgacagt ggttcctatg ttggcaccaa
    23401 atatgatgcc aatagactgt tgcagtgtta aaaggccagc actaacaaag cttaccagca
    23461 ttactgtggt ggcgcttgat gactgaataa tagatgtgat aaataaacca gtgaagactc
    23521 ctttaattct gttattgctg actttccata atataccttt caacctatca cctgctgcct
    23581 gttgcagtga ttcgctcatg attttcattc caaataaaaa caatcccaat ccaccaaaga
    23641 ttgtgatacc tattgaaaat ccatccatag tgtataacct cgagttaata atttatattc
    23701 atatacaaat agtaaaacat tattctattg tggcatagca agcttatctt gttcttttac
    23761 ttcattttct ttaagccgca catcatacgc tgattgatta atgggtaatg gaaaactttc
    23821 gcctttttcc tcgtcaagct gattggttcg ggtatcagcg tttgggttta caggctcttc
    23881 tgttttacct gaaaaacgct ctaaacttga ttgtatggac gggtcattgg ggtcaagcag
    23941 ttttgccttt tcatagtagc gttttgcttc ttcaatgttt ttcagtgatt cataacacag
    24001 gccaatattg aagaataatg atgctgttgc catattcatc tggtgggcct gcagcaaata
    24061 attcagcgcc tcagtatatt tgccctgtga taccagcagt attccaagct gatacagaac
    24121 atcaggatta ttagggttca ttcgcattgc attattaagg tatttaaatg cattgtccat
    24181 attctgaatg gttgcgctta taatgccgat gcgatagttt gcagtaaaat tattgggatt
    24241 tacagcaagc aatgcggaat ataatgaaat agctttatcc cattgcttgt ccttttcgta
    24301 catcagtgca atattttcat agagtttttc cttgtatgca attacattaa taatgtattg
    24361 ccgtagtgaa ggtgtatatt cacctgtccg ccgcaattcc atgagtgcct gcatctcacg
    24421 ttcatattgt tcctgtaatc cataatattc agactgcttt aaaagctgat taattcgctt
    24481 tgcatagaac atacgaagtt gcagtgtatt agcccagtat ggagttttat cggcaagccc
    24541 tgtaacggca tcattggtat taataacagt atcaataaag ctgtttgggt atattgtggc
    24601 atctggttta ctaaacttta gcattacagt actgttttcc ccatcgtcag gtaataccga
    24661 ttgtacagat gtactaagta tataggataa tgcatgctca ggattgatga aaaggtttga
    24721 aacttctgtt gtctgaatga ggttttccaa tgtttgcatt ttggcctgat caatagcaaa
    24781 tccgtcctgt ttatctgttg cgccaataat cataagattt ccgtatatgg caaccattga
    24841 atgtgtaaaa gatttttgca aggctttgcc aagactatgt aattgctctg gtgctatatg
    24901 ggttatatca ataatagtaa agtagtaatc atcaacatgt ggtttaatgc tggcaatgta
    24961 attatcagta aagtgaaagg catacatgta atggtcaaaa atgttaggga tatcaataat
    25021 ggtccctttt ttgtctgata atccacggag gatatcgctg ttgatggtgg catactgctg
    25081 gacaccagtt attgttggtt tctggtaatc aacaatacgc tcagggatgt aatcaataat
    25141 aacaggggca ggtaatatat ggtacaggtt gttggtaaag aatttatgat agccatcaat
    25201 aatgatggtg tgtccggaac ctgcaaaaag gcctgatagc accgttgccc gtttcatatc
    25261 gcgtgcaaag tgatcggtaa ccacacatgc aggtatcccc tgtatatatg ctgtagcagg
    25321 ttgtgtgata tatgatgcat taaaatttgc cattgctaca gtgtcatatc cggtgcagaa
    25381 tctattgacc agtgtatttg aaaatggcag cctgaaataa atatgggtaa taataattgc
    25441 agggataaat aacaaactta caacaaaata gagtattgat ggtacagaac gttttgtatt
    25501 ttgtaaaaga tacatgccgg gaaagataat cgtgataatt gccataaggt aaatgagtat
    25561 aaaaaaccac aggtacgtta attctatcca taaaaatgca attattatgg gaatgggtat
    25621 aataaagaaa gaaaaaagta gtatagtata tcgtgactga tgattgtttt tcaggataat
    25681 ctgtttaagg gaagtaccaa atgttagtcc tgaaaaaata ctgaaaggca gagcggtcat
    25741 aatactgata tagtaaggaa tatcattgct gtaaattata ataataaaac ccaggaaaag
    25801 gaatggatag agcatctcac cataaataaa aactgtgttg tgtgttttta tatactgtga
    25861 ggcgatagtt cctacgataa agcctgccgc aatggcaatt gctgaataaa aaataatttg
    25921 caggtatagt ccataccggg aaacgatact cagcagtata aggataataa atatgcttat
    25981 gaatgagaca ttgcagtatg tgaacagtat atcgtcccgt gacgtaattg cacgttcttc
    26041 tgcaacacca cgtgaaatga ttgctggcgg gcaaaagggg agggaaagga gtatagtgaa
    26101 cacacaggat agtatgggta atacaacata aagaataagg tagtgttgat tataaaatat
    26161 gaaaagccca aacgctaagg atgcaataaa cgatagcaga gcaaaacata ctgctaaaat
    26221 agcaggattt ttatcatcga tataatcgcc aatggtagtc tttaagtaat atggggcttt
    26281 tatacctgta gaaaaatata gtaaaaatat cgccccatat agcagccagt aacaatgttg
    26341 aaggatagtt ccatatgcat tgaggatatc attgtacaca aaggcagcta tgacaggagc
    26401 tatcgccaca catactgcaa taaattcctg tataataaaa ataatcctgt ggttttgtaa
    26461 tcgttttaaa atactgttgc cagcaattat accagccagt aaccctgaaa atgcaaacgt
    26521 taaagtgtat ttccagacag gggaatacag tgtcagtgag cttacgtaca ggatattaag
    26581 tagggtaaat actaccaggg tggcaattga gaaaaatacc gaaagtaaca caaatcgccg
    26641 ctgggttata ctggaatagt tgaattcttt tatgttcatt tttttgaata cgttctcctg
    26701 catactactg aatttattgt taaccccttt aaggttatgg tatctcaatt cttgattgat
    26761 actataatga cttgatgact aaaaccgcag tctttttagc aaattactga tacaattggc
    26821 actgctgaat gcatttttat gacgtagtat acgctatcga taaaaatgac aagaattttt
    26881 ctttagtaaa taattattaa gattttttca tgattttacc gtaaattata tatagaagct
    26941 tttggacttt ttttgttgca gtattttatt aattttgata tatatgcaat agaatttagt
    27001 ggagcgtata tgcactcaaa aaatgcgttt gaatatatac ctgaagacaa tcttgttgta
    27061 ttatttgtat taatggtatg tacagcaggg ttatattatt tctggtggat tgcccgtgta
    27121 agccgcattt tctatgacaa tcccgttacc aacatactgc ttaccattct cacagctggc
    27181 gtgtggctca tttatctaac tattaaatat atgcagaaat ctgaaatgct caatggcagg
    27241 cagatgccct ggtatatggt attattttta cccataggct ttttgattat tcagcacaat
    27301 attaatgagc ggtatttcac cagtcaataa ctcatcagga gccattcatg attgtacgat
    27361 gcagcgaatg taatgcagcc tatcaggttg atgatgaaaa ggtagataac aagcgttttg
    27421 catttacctg ccccaaatgc aaggctcatg ttattattga taatcgtata gatatagcag
    27481 cggataaaag tattgatgat atgattatgg ctcacgcagt agaagaaaag ccaaaaaaga
    27541 tggcacaggc tccagaagag tttgatactc tgctgcagga agaaccattt gaaaaagaat
    27601 ataccgcaga aaaaatttca acaactgatg aagatttact ggcagatatt gaatcatttg
    27661 atgcaacgca agataaaaaa gacaaaagta aggatgaatt tgagcccatt gaaagcgaaa
    27721 caatagattt gtctgatttt tctgctgaag aattgaaaag tgctactgaa gaaataacct
    27781 tacctgaaga acccatagaa aaagatttgt tgcttgatga agaaattaag gctgatgaga
    27841 ttttttcaaa ggaaaaggat gttgatgaaa gtatcactat agatttagat acccttgata
    27901 tacctcttga tgaatcaaca gtaattgaag aagctgaaat gcctggtgag tcgtcagttc
    27961 ttgaaaaaga agaactactg gtggaagata tggggtttga cgatatttta gtggcagatg
    28021 aagaaatccc tcaaaaacca aaagcaaaaa aagcgcctga aaaagaacca atagatgaca
    28081 tcacgctgga tctggattca cttgatatac aactggaaga gcatgcagag gtgttgccag
    28141 gtgaacagat aattgaagaa gacgaaaagc taacacttga agatgcagga ataactattg
    28201 atgaacttat agaagaagaa aagaaacaat cacttgaaga ggaagaagac ttgaagcttt
    28261 ctttagatga aattgagccc ggtcttacgg ttgatgacct gcataaggaa cttacagaat
    28321 cagaaataga aaccttaatg tcaaaagaaa ttgaattacc cgaagtggat ctggataaat
    28381 ttgaactgga agaaggtgag attaaagaat ttgcacctga agagtttgaa tatgctgaaa
    28441 aggaaagtta tgattcaata cctgcaggtt ttgtcaattt aaccattgat tatgcattgt
    28501 catattcacg gcctggtgca gttgcacggc ttttagggtt atatttagtc acgttgatcc
    28561 cgcatattat tgtcagttcc atctatagtc ttgtatcatc tatagttgga ttttttaact
    28621 ggtgtgtggt gttatttacc ggccagttca ttgatgattt tgcagacatg cagcagaaca
    28681 cattacggta ttatatcgca attctggcat ctgcagctca gataactgaa gacaggcctg
    28741 tttatgcagg cagaccaaaa gttgagcaca gcctgcagct tgatatagta tatccttccg
    28801 agccatcacg cctgctagct tttttgagga ttacgggtat tggaatactc acagcagcac
    28861 ttcctcatct gctgttgctt gctgtgctga ctctgggaat gcttattctg attcctgttg
    28921 gccttatcag tatcattgca acaaagcgct ggccatccct gttgtttgat ttcatgtacc
    28981 ggtatctgcg ctactattcc cgggttaatg catatattat gggactggtt gacaaatacc
    29041 cttcatttat tttttgatag acatcccgca tagcattcat tacagtgccg ctataaaaat
    29101 atatatagca ctctatggat attcattcta tcatacatac tgtgttttcc agcggcatgc
    29161 tggcaaacta tatccatgat tttgagtatc gtgccattca ggaacagatg gcaactgcta
    29221 ttgcccgctc atggtatgat gaggaacaca ttgtcattga agcggggact ggtacaggca
    29281 aaacgtttgg gtatttactt cctgctgccc tgtttgcgct cttggaagat gttaaagtgg
    29341 cgatagttac tgaaacaaag gctctgcagc agcaattgat tgtaaaagat ttgcctgtgg
    29401 ttgcttctat actaaaaaac ataaccgata gttccctgga atatgccctg tgcctgggaa
    29461 gtgccaatta cccctgcttc cgcaggttgc aacatgcaat tgatactgga agttttgata
    29521 aaaaatttgc tggtgaaata gcaaatctgc aaaagctttt aaagaaaaat cacactctta
    29581 cacgctttga cgttcaggta cctgatgctc tgtggaatac aatctgccgt gaatcggaat
    29641 tatgctcaat gaataaatgc aggtataaaa gcacctgctc atatatggaa gcgcgaaaac
    29701 agtggcagaa ggcacatatt cttgtcatga atcattatct ctttttttca aatattgctg
    29761 cgtcaaagtc gctggtgccg gattgtaagg cagtggtatt tgacgaagca cattctctgg
    29821 aaaggattgc gtcaggtcag ttaggctttt ctataaatag ccagcatctt aaagaaacac
    29881 tggaacacat gatacaaaga aaaaaaggcg catcactggt agagaagtgg ctaccccacg
    29941 ggctacaaca ggatgccatt gaagtatttg acaatgcatt gcgacaggtt ca
//
LOCUS       DAORYL010000032        29791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1325_length_29791_cov_11.952314, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000032 DAORYL010000000
VERSION     DAORYL010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 29791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29791
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1325_length_29791_cov_11.952314"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PLV81_07575"
     CDS             complement(<1..364)
                     /locus_tag="PLV81_07575"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOM09997.1"
                     /translation="MKKLLEYLSPIEKVLLSFVLLILVGACILCLPVCTTGGISFVDA
                     LFTSTSAVCVTGLIVLDTGKDFTFVGQLVILLLIQLGGLGIMTFSVALLSLMGGSVSI
                     KWRFTLQNMYSDVSKLPIR"
     gene            complement(409..481)
                     /locus_tag="PLV81_07580"
     tRNA            complement(409..481)
                     /locus_tag="PLV81_07580"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(446..448),aa:Val,seq:tac)
     gene            complement(486..755)
                     /locus_tag="PLV81_07585"
     CDS             complement(486..755)
                     /locus_tag="PLV81_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOM09998.1"
                     /translation="MTKHEIITRLHLKYGISRDVVQQIVDGFVDEIVVSVQKGEKVTI
                     KGFGVFSRVKQAERKVYSPIAQREVTIPSRYVIDFKSSKLTDIEH"
     gene            complement(777..1040)
                     /gene="rpsT"
                     /locus_tag="PLV81_07590"
     CDS             complement(777..1040)
                     /gene="rpsT"
                     /locus_tag="PLV81_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOM09999.1"
                     /translation="MANIKSAEKRNRQSEKRRQRNSQVKSSIRTAEKKVRKQLESQSK
                     ENVPALFKEFVKKIDTAARKGIIHWKTAARKKSRLAKKVNAAS"
     gene            complement(1195..1722)
                     /locus_tag="PLV81_07595"
     CDS             complement(1195..1722)
                     /locus_tag="PLV81_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10000.1"
                     /translation="MKRILSLFTVLSLVIVIAQVWAYVAMDIQKWEIDRKDVVASYIH
                     TSPYKTYTLSQLKGYSNKLLNYILAIDENYKDKVIILRVTGDITRDFMLINNKLYVIT
                     DYMQKSSEDYISGAIAQLEKKYGVPQKQSEGTTTTYTFTRDETKVIAIGENKGNNYSL
                     TIYYYYKNLFRSLMQ"
     gene            complement(2237..3151)
                     /locus_tag="PLV81_07600"
     CDS             complement(2237..3151)
                     /locus_tag="PLV81_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10001.1"
                     /translation="MQQLQLAKLLNTHDHEKTLKEIKTMYTKYYSGFETIESCFFLIR
                     DLFEGKFPGYQKCNTQYHDFQHTVDATVAAVRILDGYNSKRKVMPQNIAYSLMLASLL
                     HDVGYIQEDWDTEGTGAKYTAIHVQRSVDFVKINHKKLGIDDEYIPLMALCIQSTGLS
                     AKFEEMEFISREHKIAGAMMGTADLLGQMSDREYLEKLLFLYYEFKEAGIPGYTTEFD
                     IIRKTIDFYVMTVKRMKSTLLNVQHYCLYHFDNRYGIHSNLYMVAINRNIRYIKKIIA
                     DESTNFRHKLKRGDQYQLHMLSQKTLHH"
     gene            complement(3245..3967)
                     /locus_tag="PLV81_07605"
     CDS             complement(3245..3967)
                     /locus_tag="PLV81_07605"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HOM10002.1"
                     /translation="MIRLDKDPQHIFRIYGLCGLQAGIIAKNANSVNYSDGGIEVRFK
                     EKMLIYKATGILPEHIVMLDQVHGKEIIEILRPPLKNEPFVAQADGLITTVHGVCLVI
                     RTADCVPVILYDDQNGVVSALHSGWRGCAHNITAEGIKRLLHKGAKPSSMYVLVLPSI
                     SPQAYEIGQDVASQFDGYVLQKEGKLYLNMWQHIKDTALHNDIPEQNIFISNMCTLQH
                     NTEFFSYRKGDNGRNLNFVYLA"
     gene            complement(3981..4763)
                     /locus_tag="PLV81_07610"
     CDS             complement(3981..4763)
                     /locus_tag="PLV81_07610"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HOM10003.1"
                     /translation="MIDYHVHTSLCDHATGTPQEYIEKAIEAELFEIGFSDHAPLPLH
                     LRQNITMEPEETEAYVAMLQELTDKYPVAVKIGFEVDYPLHPTFDTRYLNDRRLDYII
                     GSCHFIGDWPFDHPAFVDEYTKRDINQVYRDYYHIVYDMVATGMFDIIGHFDLVKKFG
                     YRATADLNTEIEPILQLAAQQSTAIEINTSGWRKPVKEAYPSFEIIQKMFQYNVPITL
                     GSDAHAPEEVAYNFYEALSLIKQAGYRKIVGYRKRKQYTITF"
     gene            complement(4769..5422)
                     /locus_tag="PLV81_07615"
     CDS             complement(4769..5422)
                     /locus_tag="PLV81_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10004.1"
                     /translation="MFDIFESHEYRPPVFENMQMEISDIDNDISSDIDNLLANRFDSL
                     YMKDVYKKQHVAVEIIYPRIQYNHHKVLLKSPDYFRFLLSLYPFVSDLENVAKIVIRP
                     RHVVLGDVELMGLYLHRKQILVIYLHQPHFYSLKTARFARFAEFTPLAHLLQSKVISA
                     QNSENDPYLIPPLWYMLSLIEASHSDKVDKFLLRCDTTSKLQAALNEISFFYGRHGY"
     gene            complement(5431..6417)
                     /locus_tag="PLV81_07620"
     CDS             complement(5431..6417)
                     /locus_tag="PLV81_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10005.1"
                     /translation="MGIQDIIIFTKDEEELVETLKHAIKDKYTSEYTLIEKHKEALRS
                     LATSISLYPSLLDSQRLSNQKRTMESLLDTLCSRSIPDMILHIPTKAILGRAFTIAKI
                     NFFIMLWYIVRDRDEYASFLDTLLSCIASNVFMLTAEEVYTSIIEDDTLALNIRHNAA
                     YLLARTWEHRLDYGVAEFAPILLNLWKARERLIPNFGTMMGFSELCMLSEHTSPLWLD
                     FLQRDTITEDEVYAVEEFIFDLSFEEIVFVRDYLEKHNKITVSRDELPSILQKTHIPE
                     YQGQDPRELYRSFRDRKINSRFRARSGLQGPKKTLEEYLMCFLLSSRSMVEY"
     gene            complement(6700..8070)
                     /locus_tag="PLV81_07625"
     CDS             complement(6700..8070)
                     /locus_tag="PLV81_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOM10006.1"
                     /translation="MANREHWGSKMGFVLAAAGSAIGLGNVWKFPYVTGSNGGAAFVF
                     IYLISVVILGLPIMIAELVIGRHTEKDPVGAFKQIAGGTTWEFVGYLGVLSGFFILSF
                     YSVVGGWTIGYIVKGITGSIAGLHDTAQASEIFKQYVSNPYLSVLYHVLFMLSCMYIV
                     IKGIKNGIEKWSKILMPLLFVILIALIVRGITMEGSLEGIIFFLNPDFSKITLNSILS
                     ALGQSFFSLSLGMGAMITYGSYLSREDKILHSALWIVFLDTLIAILAGLAIFPSVFAM
                     SLSPQEGPGLIFHVIPAVFARMPLGQFFEVLFFVLLFIAALTSGISLLEVVVAYITDE
                     KGWSRRKAVLVMGTIIIILGIPSALSFGVMGNMTILGNTFFDFMDKLTSNYMLPIGGF
                     FIAICLGWKYGLENTIHELDPDTRFISLKELWAFMIKFVSPFILFVVFFMLIKDDIVA
                     LLQKVF"
     gene            8346..9167
                     /gene="ccsA"
                     /locus_tag="PLV81_07630"
     CDS             8346..9167
                     /gene="ccsA"
                     /locus_tag="PLV81_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOM10007.1"
                     /translation="MILIYIITLLFIVASAYSILSIHKDMPRYVTATKLLMSIILIVL
                     LVIGYCMPVYIDSVYKIHIFTFFYLIMLFLTLLTLYMAISQWNNDYRSLSAILIPLTT
                     IISIISLFFINSPRHMTIGPDNSIMVIHIVLALLGEVLFFVAFASSVLYIIVSWQLQK
                     KSSMKFINRFPSLTVLSKLTSFSLSRSLFFFTAGIVIGIIMLFQFYGNISSGSPKEIL
                     MYVAWGIIFILWLISRSHITSNYYLSIMTIVSFLLLMILFIASNVVITSGFHSFR"
     gene            9174..10460
                     /gene="hemA"
                     /locus_tag="PLV81_07635"
     CDS             9174..10460
                     /gene="hemA"
                     /locus_tag="PLV81_07635"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HOM10008.1"
                     /translation="MKEHIPQIGMVGMSHKTAPVETRECFAFDTQVLESFVNNAKKHS
                     IQEMVYIATCNRMELYFVSNQIEEATQEVIHLLEQFAGLTREHFEPVLYKKYHKDAVR
                     HLLCVASSLDSMVIGENEIVGQVKEWYRWATQYETTGTILNKLFHQAFRTAKRVRTET
                     EIARNPLSIAFIAVELAKKIFEDLSKQSVLLIGAGEMAELILKYLTKFKIGSVTLANR
                     SLHNAQRIASEINQDATIITLAEIMESIPNVDIIISSTGAHHYMLTKDMIQEAMKKRT
                     QPVFLIDIAVPRNIDPEAGNIHDVFLYNIDDLKSIADENLKSRLNETKLAYEMIDADT
                     EEFLHWYESLQIVPLIAKIKESFETIRNNELKKYRRRKLKHLNNEDFAIIEELTHQIM
                     TKTLHIPITYLKELGANANSKEAKEKAKLLQEIFKL"
     gene            10457..11512
                     /gene="ahbD"
                     /locus_tag="PLV81_07640"
     CDS             10457..11512
                     /gene="ahbD"
                     /locus_tag="PLV81_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019241290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme b synthase"
                     /protein_id="HOM10009.1"
                     /translation="MNGPRLIFWELTKRCNLRCVHCRAESYDREYINELSTQQIHNVI
                     DTIASNYRPIIVLTGGEPLYRKDLFDIAAYAHEKGLPIALATNGTLIDEQIAQQIKAS
                     GFQRVSISIDGPDAHTHDSFRGIPGSFDKALHGALLLYKAGVPFQFNTTITKRNVNSI
                     EAILKLAQEKKAAALHVFMLVPVGCGVEIADTDMLSKEQYEEVLRWFYYKTKEAGIEF
                     KATCAPHYYRIIRQEAKKEGRKLTFETDGLAAVTRGCLAGSGVCFISHRGDVQPCGYL
                     PLVAGNVLQTPFNQIWESSELFANLRNLSKLKGKCGLCEYKAFCAGCRARAYYATGDY
                     MDEEPYCIFEPKRMTHA"
     gene            11505..12476
                     /locus_tag="PLV81_07645"
     CDS             11505..12476
                     /locus_tag="PLV81_07645"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10010.1"
                     /translation="MLDTKQLLKTVQIQVPLVTRPFSAIADNLHSTEDEVIEALASLK
                     QKGILRSIAGIFNAKKLGYQSNLVAFVVPHNNIEEAVRVINPLPGVSHNYLRDGDYNI
                     WFTLALPQEENFSDYVEELAKKAGAMRYNIFKATTMVKLSARFDPDDVISDPQFTVKN
                     PDESITIDNTVKQAIRVLQQDLPLVAEAFGAIVTEKKIPIDVDKVLATGEKLLAAGVM
                     RSYRAVVRHHAVGFIANAMTVWKADNNKLSDLLREFSIEPSISHLYVREAFPNPWEYP
                     VFAMVHAKSQEELSAIIQKLHSIAKVDYRIYRSLQEFKKERVKYFEH"
     gene            12563..13831
                     /locus_tag="PLV81_07650"
     CDS             12563..13831
                     /locus_tag="PLV81_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOM10011.1"
                     /translation="MIDIGKLYCSGSSTGDGLRYGTEATVDAHGNLPHKRVELASQRR
                     PIVVWNVTRTCNLNCVHCYTDSHNIKYDELTTDQGLKLIDDLAAFSIPSLLFSGGEPL
                     MRKDIFTLIERASNRNIRPVLSTNGTLIDRDKAHAIKDAGIVYVGISLDGMEDVNDRF
                     RGMDGAFKKAMKGFEHCIAVGQRVGLRLTLTRRNFEDLHRIFDFIEKEGINRACFYHL
                     VYSGRGGNLFADDLTYLQQRQALDIIIERTDDFFRRGLDINILTVDNHVDGVYLYRKL
                     LQKDEQKANQVKDLLTWNGGGAYSTGVGIANIDFVGNVHPDQFWQDYTFGNVLERNFA
                     DIWMDETDPLMKGLKHKAEYIKGRCRVCQYKAMCNGSMRVRAYRVFGDPWAPDPQCYL
                     TDEEIGLTDKTIAELKSNGEYFEMPAELKK"
     gene            13840..14475
                     /locus_tag="PLV81_07655"
     CDS             13840..14475
                     /locus_tag="PLV81_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HOM10012.1"
                     /translation="MLYPIMYKIKGKRVVVIGGGQVAFRKVKDLANCGATVVVIAPDI
                     HPELEEYAAANPAITIIKREYQAGDCAGAALVFSATNNPRVNRTVYEEALALNIPINA
                     VDDPDNSSFYVPSFFTRGPLLVAVSTGGASPAMAAKVRRLIEQSIPQDIEITLDTLNE
                     ARTILKEHYHHLTSHQRSELLKAIVTDERLLEQAVEAFKNNMLQEFLKSLL"
     gene            14569..14895
                     /locus_tag="PLV81_07660"
     CDS             14569..14895
                     /locus_tag="PLV81_07660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOM10013.1"
                     /translation="MNIKHEIKDRVLIISIEGELISPQALDTDKHIIALIEKEKKERV
                     IIDLTKVDFIDSTGLGILVTIRLYCKKNKILFATVGVNEKIKRTFTVTEMTSFLNIHD
                     TIDKLL"
     gene            complement(14914..15906)
                     /locus_tag="PLV81_07665"
     CDS             complement(14914..15906)
                     /locus_tag="PLV81_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOM10014.1"
                     /translation="MIAEYIAGVDIGGTKIIATIADATGIKARVYQHTQLQGDILTIP
                     KQVYYLIQKTAEYAGIHYEEVKAVGISTCSPFEKKGNYKEIVAPNLCGGLTGNDRLPN
                     SWHSIPLEEFFNGLYSHVVVENDCVAAVVAEKLFGAGKLYDNLVYVTWSTGIGAGAYV
                     DGKLIRGKNGNAPHFGHIYMLEDGPQCGCGNYGDLEAIASGTAIARDYGVHSAKEVFD
                     NYRRGDTKAIHIVHRAARNFARGLASLNALFDTEYIVLGGSVMNDSDILLPLMREEFY
                     RSFPVLSKSVIIEKSVLFEYIADISALSLVMPSSWIESWRTYMPWKNAPELIKL"
     gene            complement(15919..16302)
                     /gene="queF"
                     /locus_tag="PLV81_07670"
     CDS             complement(15919..16302)
                     /gene="queF"
                     /locus_tag="PLV81_07670"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HOM10015.1"
                     /translation="MTDYSKAKDALIEKVEGYVFDTIPARGHCVVVQWEYPEFQSLCP
                     VSGRHDQGTLLLVYEPKHLLLESKSVRDYLSAWRNKSIWQEYVTDVIAQDLYNAIKPK
                     WLSVEIQWAPRGGIFATTTSVKGKK"
     gene            16569..17474
                     /locus_tag="PLV81_07675"
     CDS             16569..17474
                     /locus_tag="PLV81_07675"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HOM10016.1"
                     /translation="MKSNNLLKPLTLIFTMILLFPIVLYGQEVPENTALQNESNWPTI
                     PTLAMFVAVPTITILYGMETWDWGEQSDWRWAHEGFFGQNTDSGGADKIGHGWAHYLA
                     FRIFHNYYDWSENGRNTKWIYSIATATFLGVGIEVGDAFTGKYGFSYEDVVADMLGIG
                     LGILLEYSPTLDSLIGFSWQYWPTESYFDRWGKNPLHMSSDYSGAKYVFNFRLAGLQN
                     LGIKIPNFMRYIQLDIGYFTRNYVRWDETTADPYRSMYYGISLNFMEVVKDFFENPDS
                     KASRILQQPFKYYHIPAGYFVEDKI"
     gene            complement(17632..18120)
                     /locus_tag="PLV81_07680"
     CDS             complement(17632..18120)
                     /locus_tag="PLV81_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10017.1"
                     /translation="MHFETTTCISLQHIAILEEFAHKLGIPLRTIIVYMIMYAAKKEK
                     KQYRAFKRIAYRKRNADNPWKRVHLVLYQSEYEFFLDVKKLWKMSLAHVIEFCVDNVL
                     VEFFEYFKVRLAKMNSDNYPENLPSYYENRSYTFDFYCENGIHCLKFYWGPPPEALYK
                     AI"
     gene            18389..20251
                     /locus_tag="PLV81_07685"
     CDS             18389..20251
                     /locus_tag="PLV81_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOM10018.1"
                     /translation="MQEITGTSNKVIEVDLTTQSHREIPISEKDLELYLGGKGLGLKL
                     FYDRVPAGIDPLSEENEFIVMTGVYLGTGAPCAGRFAAVTKSPLTGLMGTGSCGGPFG
                     MALKTSGWDGLIIKGKAKSPVYLVISSKGVEFKDAKKFWGKDTQTVQKALEKEGSGAL
                     VIGQAGENKVLYANICSGHRFLGRCGMGAVLGSKNVKAIVAKGNEFKIVPKNPRKFDK
                     ARKKTLKFINRNPITSVSYRQFGTNANVNLNNAGGLLPVRNFTGGSHGEAHKISGEEM
                     AERFDTKFSTCKPCAILCGHKGNINGEIRQVPEYETIGTFGSNLEIFDPVAVSEFNEL
                     CTKYGLDTISTAVTIAWAMEATEKGLIKTDLKFGSPDNVAKTITDIAYRRGFGNDLAN
                     GTRKLSQKYGGADFAIHVKGLEMAAYDPRGAWGQALSYAVANRGACHLSTASFAVEVY
                     LNMHDPFSLKGKGSMIAFLEDVTCAINSMHVCQFTAFAVELEPFVVKYTPTFLLNPMV
                     TYLNPIATKFMDVSLWPEMWSGITGKRMGMFKWIKAGRRIHVLERYMNTREGISRKDD
                     TLPQRFLTEGRHCDPQQRTVPLEELVDSYYKARGFDENGIPKDRTLKRLKIEKK"
     gene            20264..21406
                     /locus_tag="PLV81_07690"
     CDS             20264..21406
                     /locus_tag="PLV81_07690"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOM10019.1"
                     /translation="MIPYNFTFLSHPKICSGLKALEHIPFELESLNARKPLVITSARW
                     AKKGLAKTLTKALYDSNITIGALFTNAPNYPSVTIINELSKLYRDRGCDSIIALGSGG
                     IAHIAKAVNMVVSLKTDILQCTDLTISSPLKPLICVPTADCEGGEMAAVAYYERFEKQ
                     SDFLYPDVIVIDSRITRHATSQEIANCALTSLAHAIEACGIVAPNPINDAYAHTALSF
                     IYQYFPKTIKKPCYKDGSVAIVNAAACADTAFSNAPQGIVHTLAQAIALDTNLQPGIV
                     MGILLRYSLAAKLHAKGGIRPELLLALAGIDTYAKTPEKDRQKAAYDYLTKCIDATCN
                     YVPSTLKGLHIPHYKLKEYARLAAAKNKQHKENEYMALLEKSFEGK"
     gene            21429..21971
                     /locus_tag="PLV81_07695"
     CDS             21429..21971
                     /locus_tag="PLV81_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10020.1"
                     /translation="MNALQYPEIKPKKKFIKRIYIAVMLWVVGRAIQAAAKVDREVKE
                     EFAKLKDNFLLKMWIWPNGPAMYVGKTKDGKVKYLGWKDKGYKPTLTMTIKSVEAAML
                     LFTFQEGTAISTARNRLVVDGDLPDACALVRILNIVEVYLLPKIIAKLAVKRYPKWSQ
                     MNPLRKHFGRILIYVRAFTF"
     gene            21994..23244
                     /locus_tag="PLV81_07700"
     CDS             21994..23244
                     /locus_tag="PLV81_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOM10021.1"
                     /translation="MQLPAYYEFCNSVKTIVGHKALEQIPQSLKAINAQRPMIITDKG
                     VEQAGLIKIVQAAMQKSKLKAAALFDSVPADSDVKVVNEVAKIYRAKKCDSIIAVGGG
                     SVLDTAKGANILVSENAADLMQFVGYGNVKRKLRPFVAIPTTAGTGSEVTSVAVIANH
                     EKNIKMAFGSLFLLPDIAIIDSRMTKTLPPTITAFTGMDALTHAVEAYFCLAKNPLSD
                     TTALAAIQLIAQNLVNVVKKPSDLDGRLALALASNLAGMAFSNSMVGLVHNIGHAIGG
                     VCGVPHGVCMNILLPYGLEYNMHKRSQYIGELLYPLAGEEVYNKTPKKERPLKTVEYI
                     RKLIQDLHDATDGKHPRFLKEVYDRTGKQMIPKEKLPDIARTAMSDPAQFYNPEDLDY
                     NDYIMVLEAAWEGIPLDRKKIKKG"
     gene            23307..25148
                     /locus_tag="PLV81_07705"
     CDS             23307..25148
                     /locus_tag="PLV81_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOM10022.1"
                     /translation="MKVELHSYNSKIKNFTLQFILRTEGISYFIIVPILLLFLSINIT
                     FTYNQLIFFIICIAFAFPISFITTQINNIIVTKPVQKYFNAVVKNEAIPEESYELAFK
                     RFLSLPYLHSIGAFFRWVFGLSMFTVPITLSQQFSATQVFISWMSILICAPSGTVLYF
                     LLTEIFIQEIYNTGIFPKIPENFTFKNTMGITKKLMISITIIVLTPFFIMIAFIATIL
                     DQGAIHLSSSWWKLFIFGSIGLLFSLFLAKLLAKTLIMKTNIIKEFLFAVGKGQLAAY
                     TKKIAVSDELAEINIAVYNMKENLRRMVELIRSSSLELKATSNNMKVSSTKFSEASRD
                     LTAIIEETSSAYEEMSSSFEMIVNTIKLQLEQANTVNTEITVINSSGANLTHQVNELT
                     GTFNEAINGVEKGKETIHRSVSAITDISQYLETVESTISTINEVADQINLLALNAAIE
                     AARAGEHGRGFAVVADEVNKLADQTASLVKSIQSTIEQYSSKIKNEIKFINETAALFE
                     SVRDKMLATQNVLNITHQFTKELTTQNESIMGKIEKLVQLSNEIYNTSNEQQITIDEL
                     TKAINSINEVATHTAEESFTIQSLSEKLEDNARRLLENIEYFKISYE"
     gene            25340..25984
                     /locus_tag="PLV81_07710"
     CDS             25340..25984
                     /locus_tag="PLV81_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOM10023.1"
                     /translation="MNTKSNIIEAINVRYSNLAHSSCCLSCGGAINHAYPQPGEVCVD
                     LGSGRGTDVLRMADAVGSNGYVYGIDISTGMIAKSRELSDKFGYTNVAFIQSDLETLP
                     LPENSCDVIISNCTINHVNNKQKVFNEIYRILKPGGRCIISDIYSIGQVPLEYKNNPD
                     AVAECWAGSIPKDEYLAIIAQAGFPEIVILEESEPYPKGAIYVASFTYKIVKPQ"
     gene            26168..27511
                     /locus_tag="PLV81_07715"
     CDS             26168..27511
                     /locus_tag="PLV81_07715"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOM10024.1"
                     /translation="MKLSQYTIIRKLHDSSNYFIMNPLSMQADIISQQEMEAIVNGTY
                     DTSVLKQKGYMLDEQKEKMLYRKAYLAFLDTHDSSEVQIFFVPWYACNFACSYCYQSG
                     YDNKGTVTPEIIDAFFSYITTHITKPKYITLFGGEPLLDSPHARKIISYFIEKANQHS
                     LHLAIVTNGYTLSSYIDILKKATIREIQVTLDGTEDVHNARRFTKTKTPTFDAIVTGI
                     DTALKHNIPINLRVVVDAQNINDLPNLAQFAIDKGWTNNPLFKTQIGRNYELHYCQAD
                     NAKLYDRASLYEKIYELIIQHPHILQFHKPAFSISKFLFEQGAMPNPLFDACPGCKTE
                     WAFDYTGKIYPCTAMVGKKGEEVGTYYPTVSLNTTVIEEWEKRDVTSIPQCQQCSLSL
                     LCGGGCAAVAKNKHGNILQSDCRPVDTLLSMGLSLYFEKEIGNYHAKSCNNQKSM"
     gene            27560..28852
                     /locus_tag="PLV81_07720"
     CDS             27560..28852
                     /locus_tag="PLV81_07720"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOM10025.1"
                     /translation="MSEKIKYIDASQFEKEVLRGGNVIVDFYSTECPPCEALAAKFEP
                     LSEIFGDDITFIKIFRQENRALAESLGVTSSPTVLFYQNDREIGTRLSGSIKRSELLD
                     TIKLLLPQERYSQLLSAIQPVTTYTDILILGAGPAGLTAGIYTAQAKLNTIIVDTQLP
                     GGQVQTTHMVSNYPGFAKPVEGFMLMHYMSEQAKEAGCQFRVAVDVTDINLRKKYIIV
                     DGIETIHAKKIIVASGCSYRELNVPGEYEYKGKGISYCATCDAKYYQDKHVVVIGGGN
                     SAIEESLFIAKFARHITIVHQFDRLQANREAQEKAFASEKISFMFSHEPRAFIKNDDG
                     SMTVEVENLKTGNRSFIACDGVFIFAGMKPNMVNEDLQNNNGYIITDEFMHTSLPDVF
                     AAGDIRYKPYRQITTAVSDGTIAAITAVRELEEATVAV"
     gene            complement(28856..29242)
                     /locus_tag="PLV81_07725"
     CDS             complement(28856..29242)
                     /locus_tag="PLV81_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10026.1"
                     /translation="MKHIHKYLPGIVNTSCIVLFFFTIAVLTQCSSYITKEEKLKLKN
                     YEGEYILLQDAVRNDIKIIKGERVRVKITATDDYIKVYAMPANVDIVKGEWLLILYLF
                     PDDFEKEKFDVKVFEDRLYQVAKPVK"
BASE COUNT         9471 a         5815 c         5611 g         8894 t
ORIGIN      
        1 tgcgtatggg gagttttgac acatcactgt acatattctg caacgtgaag cgccatttaa
       61 tagaaacact tccgcccatg agcgaaagca gtgcaactga aaatgtcata attccaagac
      121 caccaagctg gataagcaaa agaataacca gctgcccaac aaaggtaaaa tcctttcctg
      181 tatcaaggac aatcagcccc gtaacacaca ctgccgaagt ggatgtaaat aatgcatcaa
      241 caaatgaaat gccgccagta gtgcatacag gaaggcacag aatacatgca ccaacaagaa
      301 ttaatagaac aaagctaaga agaacttttt caattggcga aagatattcc agtagctttt
      361 tcatagactt ataaaataag ggtatacaag agtataccct tttacctttg ggcgctagag
      421 gatttgaacc tctgaccctc tgcttgtaag gcagatgctc tcccagctga gctaagcgcc
      481 ctttgctaat gctcaatatc ggtgagcttg cttgatttaa aatctataac atagcgtgaa
      541 ggtatggtca cttcccgctg ggctattggc gaatacactt tacgttctgc ctgtttaacc
      601 cttgaaaaaa caccaaaacc ttttatggtt accttctccc ccttttgaac cgataccaca
      661 atttcatcca caaagccatc aacaatctgt tgcaccacat cacgggagat accatatttt
      721 agatgcaacc gggtaattat ttcatgtttg gtcaaaaaat acccctgtct gcacaattat
      781 gatgcagcat taaccttctt tgccaaacgg gactttttac gtgcagctgt cttccagtgt
      841 ataattcctt ttcgtgctgc agtatcaatc ttcttgacaa actccttaaa cagtgcaggt
      901 acattttctt tggactggct ttctaactgc ttacgcactt ttttttcagc agttctgata
      961 gatgatttta cctgcgaatt tcgctgcctt cgtttttcgc tctgtctgtt ccttttttca
     1021 gctgatttaa tattagccaa tgttacaccc tcgtcactct ataatgattg ttataatttt
     1081 cttattgaat ttaatgaatt ggatataaac aaattctatc acaatgtgtc aagaatttaa
     1141 taaaattttt cgtaaaaaaa gaacacaaaa tacactctgc caaacttgct gattttattg
     1201 catcaggctg cgaaataaat ttttataata ataataaatt gtcaaagaat aattattacc
     1261 tttattttcg ccaatggcta ttacttttgt ttcatctcgg gtaaaggtat aggttgtagt
     1321 tgtaccctca ctctgtttct gtggcacacc atattttttt tcaagctggg cgatagctcc
     1381 tgaaatataa tcctctgagg atttttgcat ataatcggta ataacatata atttattgtt
     1441 gataagcata aaatctctgg tgatgtcacc tgtgaccctg agaatgatga ccttgtcttt
     1501 ataattttca tcaatggcaa ggatgtaatt tagcaattta ttggaataac cctttaactg
     1561 gctgagtgtg tacgttttat agggcgatgt atggatataa gatgcaacta catccttcct
     1621 gtctatctcc catttctgta tatccatggc aacatatgcc cacacctgag ctatgacaat
     1681 aacaaggctt aataccgtga ataatgatag tattcgtttc atatatagtt gtgctccagt
     1741 tacttaagac tacataatga tctacccata cctgggtttt agtacgtttt tgcaagctcc
     1801 tataaaaaca attccacgat tgcaagtaac aaatgcacgt tttgtgacat ctggtaaatc
     1861 agttaaagta ttgcctgata aactattatt gtcaaacatt tttctgcaat agtaatgcgt
     1921 gagggatacg aagggtgccg tacggcagcc acgggcgtga cgggtgattg taggagcaca
     1981 tcgtgatgta cccaaaagca tcacggtgaa gcggagtatg aagataacca tcatggtgta
     2041 tatgtgatac cccgtcacgc ccgtggccga aggcgaaagc ctgagcccgc agtagcccga
     2101 ccccgccgtg ttgattggtg tgtaatatct gaatattgta ggggcgcatt tctatgcgcc
     2161 cctttgttaa aaaagtgtca attggagtag tacggcgggg gcacgcccaa aataatacaa
     2221 aatagacgca attcttttaa tgatgtaatg tcttttgtga tagcatatgt agctggtact
     2281 ggtcgccacg ttttaattta tggcgaaagt tggttgattc atcggcaatt atttttttta
     2341 tatacctgat attgcggttt attgccacca tatagagatt gctgtgaatg ccgtaacggt
     2401 tatcaaaatg atacaggcag tagtgctgga cattgagcag agtacttttc atgcgtttga
     2461 cagtcatgac ataaaaatca atggtttttc ggattatatc aaactcagtt gtatatcctg
     2521 gaataccagc ttctttgaac tcatagtata aaaataatag tttctcaaga tattctctgt
     2581 cggacatctg ccccagtaga tcagctgttc ccatcatggc tcctgctatt ttatgctctc
     2641 gcgaaataaa ttccatttcc tcaaactttg ccgaaagacc tgttgactgt atgcataatg
     2701 ccataagggg aatgtattca tcatctatgc ctaatttttt atgatttatt tttacaaaat
     2761 ctacacttcg ttgaacatgt attgctgtat attttgcccc tgtgccttcg gtatcccagt
     2821 cctcctgaat atagccaaca tcatgtaaca gtgatgctaa cattaatgaa taggctatat
     2881 tttgcggcat gacttttcgt ttactattat agccatcaag tattcgtact gcagcaactg
     2941 ttgcatcaac ggtgtgttga aaatcgtgat actgtgtatt acacttttga tatccaggaa
     3001 acttgccttc aaataagtcc cttattaaga aaaaacatga ttcaattgtt tcaaaaccgg
     3061 aataatattt tgtatacatt gttttaattt ctttaagagt tttctcatga tcatgcgtat
     3121 tgagcagttt tgcaagttga agttgctgca tactttcctc cagagtatga atatctatag
     3181 tatcatttaa gttagaaaaa atgcaaaaat ttttctatgt atgtatttaa aaaaatttat
     3241 atactcaggc taaatagaca aaatttagat ttctgccatt atcccctttt cggtacgaaa
     3301 agaattcagt gttgtgctgt aatgtgcaca tatttgaaat aaatatattt tgttctggaa
     3361 tatcattgtg cagtgctgta tccttaatat gttgccacat atttaaatat agctttcctt
     3421 ctttttgtag tacatatccg tcaaattgag atgcaacatc ctgaccaatt tcgtatgcct
     3481 gtggactaat tgaaggtaaa accagaacat acatcgatga aggctttgcg cctttatgga
     3541 gtaaccgttt aataccttct gcggttatat tgtgcgcaca acctcgccat cccgagtgca
     3601 gtgctgatac aacgccattc tggtcatcgt acaggataac tggcacacaa tcggctgtcc
     3661 gtatgacaag gcatacacca tgaacagttg taataagccc atcagcctgt gctacaaaag
     3721 gttcattttt aagaggtggc cttagtattt ctataatttc ttttccatga acctgatcaa
     3781 gcatcacgat atgttctggt aatattcctg ttgctttgta tataagcatc ttttctttaa
     3841 atcgtacttc tatcccacca tcagaataat tcactgaatt ggcatttttt gcaataatgc
     3901 cagcctgcaa tccacataaa ccatagattc tgaaaatgtg ttgagggtct ttatccagac
     3961 gtatcatata tgattcttta ttaaaatgtg attgtatatt gttttctttt tctgtaacca
     4021 actatttttc tgtaccccgc ctgttttata aggctcaatg cttcataaaa attatatgcc
     4081 acttcttccg gagcatgggc atctgaaccc agtgtaattg gaacattata ctgaaacatt
     4141 ttttgtatga tttcaaatga tgggtatgcc tctttgacag gctttcgcca tcctgacgta
     4201 tttatctcaa ttgcagtaga ttgctgtgca gctaattgta atattggttc aatttctgtg
     4261 tttaaatcag cagttgccct gtaaccaaat tttttaacca ggtcaaaatg cccaataata
     4321 tcaaacatac ctgttgccac catatcatac actatatggt aataatcacg atatacctga
     4381 ttgatatccc gtttagtata ttcatcaaca aatgccggat ggtcaaatgg ccaatcgcca
     4441 ataaaatggc atgaaccaat tatataatct aaacgcctgt catttaaata tcgagtatca
     4501 aacgttgggt gcagcggata atccacctca aaacctatct ttacagccac tggatattta
     4561 tcagttaatt cctgcaacat ggcaacatat gcttcagttt cctctggctc catagtaatg
     4621 ttttgcctta agtgcaatgg caaaggtgca tggtcagaaa aacctatctc aaataattct
     4681 gcttctattg ctttttcaat atattcctgc ggagttcctg ttgcatggtc gcacagggag
     4741 gtatgtacat ggtaatctat catgtaaatt aatatccatg acgtccataa aagaaagata
     4801 tctcattcag tgctgcctgg agtttggaag tagtatcaca ccgcaataaa aacttgtcaa
     4861 ctttgtcgga atgagatgct tcaataagtg aaagcatgta ccacagtgga gggataagat
     4921 atggatcgtt ttcagaattt tgtgcactga taacttttga ttgcaacaaa tgtgccagcg
     4981 gggtaaactc agcaaaccgg gcaaagcgtg ctgtttttaa tgagtaaaaa tgtggctggt
     5041 gcagatagat aacaagaatt tgttttctgt gcagatacaa tcccattagc tctacatcac
     5101 ccagaacaac atgacgtgga cggattacta ttttggcaac attttcaaga tcactgacaa
     5161 acggatacag tgatagtaag aacctgaagt aatcaggtga ttttaataat actttatggt
     5221 gattgtattg gatgcgggga tagataattt caacagccac atgttgtttt ttatacacat
     5281 ctttcatata caatgaatca aacctgtttg ccagcagatt atcaatatca gaggatatat
     5341 cgttatcaat atcgctgatc tccatctgca tattttcaaa tactggcggc ctgtattcat
     5401 gtgattcaaa aatatcaaac atggcatctc ctaatattca accatactgc gagaagataa
     5461 taaaaaacac atcagatatt cttcaagcgt ttttttgggg ccctgcagcc ccgaacgagc
     5521 cctgaagcgc gagttaatct ttcggtccct gaatgatcgg tataattctc gcgggtcctg
     5581 accctgatat tcagggatat gagttttttg caaaattgac ggcaactcat ccctgcttac
     5641 cgtgatttta ttatgctttt ctaaataatc ccttacaaaa actatctctt caaaacttaa
     5701 atcaaatata aactcctcaa ccgcatatac ctcatcttcg gtaattgtgt cgcgctgtaa
     5761 aaaatcaagc cataatggcg atgtatgttc cgatagcatg cataactcag aaaaacccat
     5821 catggtgcca aagttaggga ttaggcgttc gcgggctttc cacaaattaa gcagtattgg
     5881 ggcaaactca gcaactccat aatcaaggcg gtgttcccag gttcttgcca gcaaatatgc
     5941 agcattatga cgtatattca atgcaagcgt gtcatcttct ataattgatg tatatacctc
     6001 ttcggcagtg agcataaata catttgatgc aatacatgag agcaatgtat caagaaatga
     6061 tgcatattca tctctatccc ttacaatata ccacaacatt atgaaaaaat taatctttgc
     6121 tatggtaaat gcccgtccta aaattgcctt tgtagggata tgcaaaatca tatcaggaat
     6181 tgacctggaa cataaggtat ccagcaaact ctccattgta cgcttttgat tactcaacct
     6241 ctgtgaatct aatagcgagg ggtataacga aattgatgtt gctaaactcc gtaatgcttc
     6301 cttatgtttt tctatcaaag tatattctga cgtatactta tctttaatag catgttttaa
     6361 tgtttcaaca agctcttctt catctttagt aaatatgata atatcctgta tacccattga
     6421 atcaaccctc acctataaat aaatccagtt tgagaatttt gtcaatatta aattcgtata
     6481 acaaattaat aaaaaaagac gatcccttac ggaaccgtca atatcattag cggattatgt
     6541 tggaaggtaa ctactcaatg agtagcttca ccattaattg taataaaaac ccctataata
     6601 gtcaaataaa atttttcttt ttattgataa atatttcatt gcatatcatg ccgttttcag
     6661 taacgggtat atgccatata cattatgttt acatgaaaac taaaatacct tttgtaacaa
     6721 tgcaacaata tcatccttta tgagcatgaa aaatactaca aataatataa aaggcgacac
     6781 aaacttgatc atgaatgccc acaactcttt caatgatata aaccgtgtat ccgggtcaag
     6841 ctcatgaatg gtattttcaa ggccatactt ccaccccaaa cagattgcaa taaagaaacc
     6901 acctattggc aacatgtaat ttgaggtaag cttatccatg aaatcaaaaa atgtatttcc
     6961 caatattgtc atattcccca ttaccccaaa tgaaagtgct gaaggtattc ccagaatgat
     7021 aataatggtt cccattacaa gtaccgcctt tcttctactc cacccctttt catcggttat
     7081 gtatgccaca accacttcca gcagcgaaat acccgatgtc agtgctgcta taaacaacaa
     7141 cacaaaaaac aaaacttcaa aaaactgccc caacggcata cgtgcaaaaa ccgctggtat
     7201 gacatggaaa atgagccccg ggccttcctg gggcgatagc gacatcgcaa atactgatgg
     7261 gaaaatagca aggccagcta aaattgcaat gagcgtatcc agaaagacta tccacagtgc
     7321 tgaatgcagg attttatctt cccgtgagag atagctccca taggttatca ttgcccccat
     7381 accaaggctt aagctaaaga atgattgccc caaagctgaa agaattgaat ttaacgttat
     7441 ttttgaaaaa tcaggattta aaaagaaaat tataccttca agacttcctt ccattgttat
     7501 gccacgcaca atcagcgcta tcaaaataac aaacaaaagt ggcatcaata ttttactcca
     7561 tttttcaatg ccatttttta tcccttttat cactatgtac atacatgaca gcataaagag
     7621 aacatggtat aacactgata gatagggatt ggaaacatac tgtttaaata tttcagaagc
     7681 ctgagccgta tcgtgtaaac ctgctattga accggtaata cctttaacaa tataaccgat
     7741 agtccaccca cctaccacac tataaaagga aaggatgaaa aaacctgata gcacgcctaa
     7801 atagccaaca aattcccagg tagtgccacc agctatctgt ttaaatgcac ccacagggtc
     7861 tttttcagta tggcggccaa taaccagttc tgctatcata atgggcagcc ccagtataac
     7921 tactgaaata agatatataa atacaaatgc tgcaccaccg ttagagccgg ttacatacgg
     7981 aaatttccac acattaccca gccctattgc cgaacctgct gcagccagca caaaacccat
     8041 ttttgatccc caatgttcac ggttagccat tctcattcac ctctttaaag taaatagaat
     8101 ttttgcaaag cttacgtttt tcctgaaaaa attgcgggta cacacgttac aaaatagttg
     8161 aatatacaag aattattgca atattttttt tattaatcaa atttttgcca tgaactatcg
     8221 cttatattat tttaaactac gttgatttac aagaatcaac caatagtact tgacacagtg
     8281 tcaaatgaac aataattatg aattgtatta aaatagatat atattaataa acaatccgag
     8341 gatttatgat tttaatatat ataataacat tactgtttat tgtggcatcg gcatacagca
     8401 tcctttccat acataaggac atgccacgct atgtgacagc cactaaatta ctaatgagca
     8461 ttattttgat tgtgcttctg gtaattgggt attgtatgcc tgtgtatatt gatagtgtat
     8521 ataaaataca tatttttact ttcttttacc ttataatgct ttttttaaca ttgcttactc
     8581 tttatatggc aatatcacaa tggaacaatg actacaggtc gttgagtgca atacttattc
     8641 cactcacgac cattataagc attatttcat tgtttttcat aaactccccg cgacatatga
     8701 ccattggtcc tgataacagt atcatggtca tccatattgt actggcactg ctgggtgagg
     8761 tactgttctt tgtagctttt gcttcttcgg tgctctatat tattgtttca tggcaactgc
     8821 agaaaaaatc atccatgaaa tttattaacc gtttcccaag cctaaccgta ttgagtaaac
     8881 tcaccagctt ctcactgtca cgctcacttt tcttctttac tgctggtatc gtcattggca
     8941 tcatcatgtt atttcagttt tatgggaata tatcatctgg aagtcctaaa gaaattttaa
     9001 tgtatgtggc atggggtatt atttttattt tatggcttat aagcaggtca catataacca
     9061 gtaactacta tctcagtatt atgactatag tatctttttt attgcttatg attctgttta
     9121 tagccagcaa tgttgtgatt acatcaggat tccacagttt caggtaatta ataatgaaag
     9181 aacatatacc acagattggc atggttggga tgagtcataa aacagctccc gttgaaacac
     9241 gtgagtgctt tgcctttgat acgcaggttc tggaatcatt tgtaaataat gcaaaaaagc
     9301 attccataca ggaaatggtg tatattgcaa cctgcaacag gatggaactg tactttgtgt
     9361 ctaatcaaat agaagaagca acgcaagagg taattcattt gctggaacaa tttgcggggt
     9421 taacacgtga gcattttgaa cctgtgttat acaaaaaata ccataaagat gcagtgcgcc
     9481 atcttttgtg cgttgcgtca tcacttgatt caatggtgat tggtgaaaat gaaatagttg
     9541 gtcaggttaa agaatggtac cgctgggcta cacagtatga aactacaggc actatcttaa
     9601 ataagctgtt tcaccaggct ttcagaacgg caaaacgtgt acgcaccgaa actgagattg
     9661 cccgcaatcc tttatccata gcatttattg cggttgaact tgcaaagaag atatttgaag
     9721 acctttcaaa acaatctgtc ctcttaattg gcgctggcga gatggccgaa cttattttaa
     9781 aatatttaac taaatttaag attggctcgg taacgttagc taaccgttca ttacacaacg
     9841 cacagcgaat tgccagtgaa atcaatcagg atgcaactat catcacactg gcagaaatta
     9901 tggaatctat acctaatgtt gatataataa tatcctcaac aggggcgcac cattatatgc
     9961 tgacaaagga tatgatacag gaagcaatga aaaagcgtac gcaaccggta tttttaattg
    10021 atattgctgt tccccgcaat attgaccctg aagcaggcaa catccatgat gtgtttttat
    10081 acaacattga tgatttaaag tcaatagctg acgaaaattt aaaaagcaga cttaacgaaa
    10141 caaagttagc atatgaaatg atagatgctg acactgaaga attccttcac tggtatgaaa
    10201 gcctgcagat agtgccattg attgctaaaa ttaaagaatc ctttgaaacc atccgcaata
    10261 acgaactaaa aaaataccgc cgccgaaaac tcaaacattt aaacaatgaa gattttgcta
    10321 tcattgaaga gcttacccat cagatcatga ctaaaacgct tcatataccc attacgtatc
    10381 taaaagagct tggcgcaaac gctaactcaa aagaagcaaa ggaaaaagct aaattacttc
    10441 aggagatatt caagctatga atggccctcg cctcatcttc tgggaactta caaaacgctg
    10501 caatctacgc tgtgtgcact gcagagctga aagctacgac agggaatata tcaatgagct
    10561 ttcaacacag cagatccata acgtcattga tactattgcc agtaactatc gccctattat
    10621 tgtattaacg ggcggcgaac cactgtatcg taaagattta tttgatattg ccgcgtatgc
    10681 gcatgagaaa ggtttgccaa tagcacttgc aaccaatggc acactgatag atgaacagat
    10741 tgcacagcaa ataaaggcaa gcggatttca acgggtaagt atttccattg acgggcctga
    10801 cgcacacaca cacgattcat tcagaggaat tcctggttca tttgataaag ccttacatgg
    10861 tgctttactg ctctataaag ctggtgtgcc gtttcagttc aacactacaa ttacgaagcg
    10921 aaacgtaaac agtattgaag ccattttaaa actggcacaa gaaaaaaaag ctgctgcact
    10981 gcatgtgttt atgctggtgc ctgttggctg cggcgttgag atagcggata ctgatatgct
    11041 ttcaaaagaa cagtatgaag aagtgctccg ctggttttac tacaaaacta aagaagctgg
    11101 cattgaattc aaggcaacgt gtgcacccca ctactatcgc atcatacggc aggaagcaaa
    11161 aaaggaagga cgtaaactca cctttgaaac cgacggactt gccgcagtga cgcgcgggtg
    11221 tctggctgga agtggtgtgt gttttatttc gcacaggggc gatgtacagc catgtgggta
    11281 tctgccactt gttgcaggca atgtactgca aacaccgttt aatcaaatat gggaatcatc
    11341 agagctgttt gccaatctgc gcaatttgtc aaaacttaaa ggcaaatgcg gcttatgcga
    11401 atataaagcc ttttgtgcgg gctgccgcgc acgggcatat tatgcaactg gtgattacat
    11461 ggatgaagaa ccgtactgca tatttgagcc aaagaggatg acccatgctt gacacaaaac
    11521 aactgcttaa aacagttcag atacaggtac cgttagttac aaggccattt agcgccattg
    11581 ctgataatct tcattcaact gaagatgaag ttattgaagc gcttgccagt ttaaaacaaa
    11641 agggcatact gagaagtatt gctggaatat tcaatgcaaa gaaacttggc tatcagtcaa
    11701 acctggttgc ttttgttgtg ccacacaaca atattgaaga agcagtacgt gttattaacc
    11761 ctttaccagg tgtaagccac aattatcttc gtgatggtga ttacaatata tggttcacat
    11821 tggcgctgcc acaggaagaa aatttttctg attatgttga agagcttgca aagaaagcgg
    11881 gtgccatgcg ttataatatt ttcaaagcaa caaccatggt gaaactttct gcccgctttg
    11941 accccgatga tgtgataagt gatccacaat ttacggtaaa aaatcctgat gaaagcatta
    12001 ctattgataa cacggtaaaa caggctatca gagtattgca acaggatctt cccttagttg
    12061 cggaagcatt tggggcgata gttaccgaaa agaaaatccc cattgacgta gataaggttc
    12121 ttgcaacagg tgaaaaactg ctggctgcag gtgtgatgcg ttcataccgt gccgttgtca
    12181 ggcatcatgc agttgggttt attgcaaacg ccatgacggt atggaaagca gataataaca
    12241 agctttcaga tttgttgcgt gagttttcca tagagccttc catttcacat ctgtatgtgc
    12301 gtgaagcgtt tcccaatcca tgggaatatc ctgtttttgc catggttcat gcaaaaagtc
    12361 aggaagaatt gtctgctatc atacagaagc ttcattcaat agcaaaggtt gattacagga
    12421 tctatcgctc attacaggaa tttaagaagg aacgtgttaa gtattttgaa cattgataga
    12481 gtttctatcc ctcctttcct taccagggaa aggtgcccgg agtgaggttc acacattctt
    12541 tttctttaag gagaattcaa ctatgattga tatagggaaa ctgtactgca gtggttcatc
    12601 aacaggtgat gggttgcgct atggcaccga ggctaccgtg gatgcacatg gcaatctgcc
    12661 ccataaacgg gtggagcttg caagccagcg tcgccctatt gttgtgtgga atgtgacacg
    12721 cacctgcaac ttaaactgtg ttcactgcta caccgattcg cacaacataa aatacgatga
    12781 gctaaccact gatcagggac tgaagctcat tgatgattta gctgcattta gcattccttc
    12841 actgcttttt tctggtggtg agccattaat gcgtaaagat atatttacac ttattgaacg
    12901 tgcatcaaac aggaatatca ggccagtgct ttcaacaaat gggacattga ttgatagaga
    12961 taaagcccat gccatcaaag atgcaggcat tgtgtatgtt ggcataagcc tggatggcat
    13021 ggaagatgtc aatgacagat tccgcggcat ggatggtgca ttcaaaaaag ctatgaaggg
    13081 ctttgagcac tgtattgccg ttggccagcg tgtgggacta cggcttacgc tcacccgacg
    13141 taattttgag gatcttcacc gcatatttga ctttattgaa aaagaaggaa ttaaccgtgc
    13201 atgcttttac catttagtgt attctggtcg tggtggtaat ctttttgccg atgatcttac
    13261 atatctacag cagcgtcagg cacttgacat catcattgaa cgcactgatg atttcttcag
    13321 gcgcggactt gacatcaata ttcttaccgt ggataatcat gttgatggag tgtacctgta
    13381 tcgcaagttg ctacagaaag atgaacaaaa agccaaccag gtaaaggatc tattaacctg
    13441 gaatggcggt ggtgcctatt ccacgggagt tggcattgca aatattgatt ttgttggcaa
    13501 tgtccatcct gaccagttct ggcaggatta tacctttggc aatgtgcttg agcgcaactt
    13561 tgccgatatc tggatggatg aaacagaccc tcttatgaaa ggattgaagc ataaagctga
    13621 gtatataaaa ggacgctgca gggtgtgcca gtacaaagcc atgtgtaacg gctcaatgcg
    13681 tgtgcgagca taccgtgtat ttggtgaccc atgggctccc gatcctcagt gctacctaac
    13741 cgacgaagag attggattaa ctgataaaac aattgcagag ttaaaatcca atggagaata
    13801 ttttgaaatg ccagctgaat tgaaaaagta ggatgcacta tgctttatcc aataatgtat
    13861 aaaataaagg ggaagcgcgt tgtggtcatt ggtggcggcc aggttgcatt tcgtaaagtc
    13921 aaagatctgg caaactgcgg tgctacggtt gtggtgatag ctcctgacat tcatcctgag
    13981 ctggaggaat atgctgcagc caatccagcc attaccatca ttaaacgcga ataccaggca
    14041 ggcgattgtg ctggtgcagc gcttgttttt tcagcaacaa ataatcccag ggtaaatcgc
    14101 acagtctatg aagaagcatt ggcgttaaat attcccataa atgccgtgga cgatcccgat
    14161 aattcatcgt tttatgtgcc atcgtttttt acacgaggcc cccttcttgt tgctgtttcc
    14221 acaggtggcg cttcacctgc tatggcagca aaagtgcgaa ggctcattga gcaaagcatt
    14281 ccacaggata tcgagataac acttgataca ttgaatgaag cacgcaccat attaaaagaa
    14341 cactaccacc atcttacatc ccaccaacga agtgaacttt taaaagcgat agttaccgat
    14401 gaaaggcttc ttgaacaggc tgtcgaagct tttaaaaata atatgctgca agagtttttg
    14461 aaatcactgc tataataaat aatgcttgat tttctttaaa agttttttca tattcatctt
    14521 tatggaaggg tttacaatag aaattgtgtg ccggaggtta ttatcaggat gaatatcaaa
    14581 cacgaaataa aagacagagt actgattata tccattgaag gagaattaat atcccctcag
    14641 gctttggata ctgacaaaca tatcatagct ctaattgaaa aagaaaaaaa agagcgcgtt
    14701 attattgatc tcacaaaagt tgattttatt gattcaacag gattgggaat actggttaca
    14761 atacggttat attgcaaaaa gaacaagatt ctgtttgcaa cggttggagt taatgaaaag
    14821 atcaaacgaa ccttcaccgt taccgaaatg acatcatttt taaatattca cgatacaata
    14881 gataaacttc tttgattgtc attaactgta ttgttatagt ttgatcagct caggcgcatt
    14941 cttccacggc atatatgttc tccacgattc aatccatgaa gaaggcataa caagcgacag
    15001 tgcagatata tcagcaatat attcaaacaa tacacttttt tctataataa cgctttttga
    15061 taacacaggg aatgatctat agaactcttc ccgcataagt ggcagcagaa tatcgctgtc
    15121 attcatgacg ctaccaccta atacaatgta ttccgtatca aataatgcat tgagtgaagc
    15181 cagtccccgc gcaaaatttc gtgcagcccg gtgtactatg tgtatagcct ttgtatcgcc
    15241 tctacgatag ttgtcaaaga cttccttggc agagtggact ccataatcac gggctattgc
    15301 tgtgccagat gctatcgcct ctaaatcccc atagtttcca caaccacatt gcggaccatc
    15361 ctcaagcatg tatatatgac caaagtgcgg agcattaccg tttttaccac gaatgagctt
    15421 gccgtcaaca tatgcaccag cacctatgcc tgtactccag gtaacataca caagattatc
    15481 atataatttg cccgcaccaa aaagtttttc cgcaacaact gctgccacac agtcattttc
    15541 tacaacaaca tgcgaatata acccattgaa aaattcttca agtggtatag agtgccatga
    15601 attgggtaaa cgatcattgc cggttaatcc accgcaaaga tttggagcaa ctatctcttt
    15661 atagtttcct tttttttcaa aagggctgca ggtgcttata ccaactgctt ttacttcttc
    15721 atagtgtatg ccagcatact ctgctgtttt ttgaatcaaa taataaacct gtttgggaat
    15781 tgtcaggata tccccctgca attgtgtatg ctggtaaacc cgtgctttta tacctgtggc
    15841 atcagcaata gttgcaataa tttttgtccc accaatatcg acacctgcta tatattcagc
    15901 tatcatagtg acctttattt actttttgcc ttttaccgat gtagttgtag caaatattcc
    15961 gccacgtggt gcccactgga tctccactga aagccatttt ggcttgattg cattgtacag
    16021 gtcctgtgca atgacgtcag taacgtattc ctgccatatt gatttatttc gccatgcact
    16081 caaataatca cggactgatt tgctttctaa aagtaaatgt tttggctcat acacaagtag
    16141 taaggtaccc tgatcgtgcc tgccagacac cgggcacaaa ctctgaaatt caggatattc
    16201 ccactgaact accacacaat ggccgcgtgc aggtattgtg tcaaagacat acccttcaac
    16261 tttttcaatt agtgcatctt tagcttttga ataatctgtc atggtcattc ctttcgatga
    16321 aagtctactt atatgtaata tagctataag taaaaattac aacaaaaatt catcgtttat
    16381 gcactcatta ttactgtact gttattttgt ttatacattt atttttttat atttatttta
    16441 ttaaatttca ttgacatatc tgattttaaa cttttctgtg ctttttaata ttctgcttta
    16501 tgtaattgtt tttgaatacc catagcacct gcagaatgta aagatcaatg tatgttgagg
    16561 gcattattat gaaaagcaac aacttgttaa agccactcac cctaattttt acaatgatac
    16621 tactattccc cattgttctt tatgggcagg aagtacctga aaatactgca cttcagaatg
    16681 aaagcaactg gccaacaatt ccaacactgg caatgtttgt ggcagtacct acaattacaa
    16741 tattatatgg gatggaaaca tgggactggg gtgaacaaag tgattggcga tgggctcatg
    16801 aaggtttttt tggacaaaat actgattccg gtggtgctga caaaattggt cacggctggg
    16861 ctcattattt agcgttcaga attttccaca attactatga ctggtcagaa aatggcagaa
    16921 acactaaatg gatttattcc attgcaaccg ctacttttct tggagttggt attgaagtgg
    16981 gcgatgcatt tacaggcaag tatggttttt cctatgaaga tgttgtggcg gatatgcttg
    17041 gaataggatt gggtattctt ctggaatatt ccccaacact ggactcactc attggtttta
    17101 gctggcaata ctggcccact gaaagttatt ttgaccgctg gggtaaaaat ccactgcata
    17161 tgagcagtga ttacagtgga gcaaaatatg tttttaattt caggttagca ggtctgcaaa
    17221 acttagggat aaaaatacca aacttcatgc gttatataca actggatatt ggctatttta
    17281 cacgtaacta cgttcgatgg gatgaaacca ctgcagatcc ttatcgtagt atgtactacg
    17341 gtatttctct gaatttcatg gaagtagtaa aagatttctt tgaaaatcct gattctaaag
    17401 caagccgcat cttacaacaa ccgtttaaat attaccacat acctgcagga tattttgttg
    17461 aagacaagat atagttaaat tataacacat taaaagataa catataaaac cgctttatgc
    17521 cagacagttt accataaagc ggttttttcg tcttcaatat ggctaataat catttgcata
    17581 taatgcaatg atatagaaaa aaatttccgc ctgattttat gaattttgtg cttatatagc
    17641 tttatacaag gcttctggcg gtggccccca gtagaatttt aggcaatgga taccattttc
    17701 acagtaaaaa tcaaaggtgt atgacctgtt ttcgtagtaa cttggcaaat tctcaggata
    17761 gttatcggaa ttcatctttg ccaatcgtac cttgaaatat tcaaaaaatt caaccagcac
    17821 attatccaca caaaactcaa taacatgcgc taacgacatt ttccacagct tcttcacatc
    17881 caggaaaaac tcatactcag attggtacaa caccagatgt acccgtttcc agggattatc
    17941 agcatttcgt ttgcggtagg ctatgcgttt aaatgctctg tattgctttt tttctttctt
    18001 agcagcatac ataatcatat aaacaataat agtacgaaga ggaatgccta atttatgtgc
    18061 aaattcttca aggattgcaa tatgctgaag agaaatacag gtagtcgttt caaaatgcat
    18121 aatttcctcc caaagatttt atgtgttttg gaattatttt aatggtttgc ccatttaaaa
    18181 taataattta ttttttttgt caatcataag ttttaattta taacattgta cttatttttt
    18241 atattctttg caatatcgtg attgacatat aattttacag tattctatta ttactcatat
    18301 acaattttat tgtaataata caattaatcg tttttgtttt aacgccaatc agtacatcaa
    18361 aaaaattaac gttttaagga gatactatat gcaggaaatc actggaacaa gtaacaaagt
    18421 cattgaagtt gatttaacca cacaatccca ccgtgagatt cctatctctg aaaaagacct
    18481 ggaactctat ttaggtggta agggattggg cttaaaactt ttctatgaca gagtgccggc
    18541 tgggattgat cctttatcag aagaaaatga atttattgtt atgaccggtg tgtatttagg
    18601 taccggagca ccctgtgcag gacgttttgc agcagttacc aaatcaccat tgacagggct
    18661 tatgggaacc ggctcctgtg gtgggccatt tggtatggca ctcaaaactt ccggttggga
    18721 tgggctcatt ataaagggca aagccaaatc accggtgtat ttagttatca gttcaaaggg
    18781 agttgaattc aaggacgcta agaaattctg gggcaaagac acgcagactg ttcaaaaagc
    18841 ccttgaaaaa gaaggcagtg gtgcactggt tattggacag gcaggtgaaa ataaggtact
    18901 gtatgccaat atctgttccg gtcacagatt tttaggacgc tgtggtatgg gtgcagtact
    18961 gggttcaaaa aatgttaaag cgatagttgc taagggaaat gaattcaaga ttgtacccaa
    19021 aaatcccaga aagtttgata aagctcgtaa aaaaaccctc aaatttatca atcgcaatcc
    19081 cattacatcc gtgtcatacc gacagtttgg taccaatgca aatgttaacc tgaataatgc
    19141 aggagggtta ttgccggttc gcaatttcac cggtggatca cacggcgaag cacacaaaat
    19201 ttccggtgaa gaaatggctg aacgctttga taccaaattt tcaacgtgca agccctgtgc
    19261 tattttgtgt ggtcacaaag gcaacataaa tggtgaaata cgtcaagttc ctgaatatga
    19321 aactattggg acgtttggtt ccaatttaga aatctttgat ccagtcgctg tcagcgaatt
    19381 taacgaattg tgtacaaaat atggccttga tacaatatca actgctgtta ctatagcatg
    19441 ggcaatggaa gctaccgaaa agggactaat taaaacagac ctcaaatttg gatcacctga
    19501 taatgtagca aaaaccatta ctgatattgc atatagaaga ggatttggta atgatttagc
    19561 caatggtacc cggaaactat cgcaaaaata tggtggtgct gattttgcaa tacatgttaa
    19621 aggccttgaa atggcagcct acgatccccg tggtgcgtgg gggcaggcat taagctatgc
    19681 tgttgctaac cgtggtgcgt gccatctttc tactgcaagc tttgctgttg aagtatatct
    19741 gaatatgcat gatcctttct ctcttaaagg taaaggttcc atgattgcat ttttggaaga
    19801 tgttacctgc gcaattaatt ccatgcatgt atgccagttt actgcctttg cagttgaact
    19861 ggaacccttt gtggtaaaat acactccaac gtttctcctt aacccaatgg taacctatct
    19921 taacccaatt gccacaaaat tcatggatgt aagcctgtgg ccagaaatgt ggtccgggat
    19981 taccggcaaa cgaatgggta tgttcaagtg gattaaagca ggaagaagga tacatgttct
    20041 tgagcgatac atgaataccc gcgaaggcat ttcacggaaa gatgatactc ttccacaacg
    20101 attcttaacc gaaggcaggc actgtgatcc acagcagcgt acagtaccac tggaagaact
    20161 ggtagatagt tactataaag cacgtggctt tgatgagaat ggcattccta aagacagaac
    20221 attaaaacga ttaaaaattg aaaaaaaata atgaggtgac accatgattc catacaattt
    20281 tacattttta agtcatccaa aaatctgttc agggcttaaa gcgctggaac atatcccatt
    20341 tgaattagaa tcactgaatg cccgcaagcc ccttgtaata acatcagccc gatgggcaaa
    20401 aaaaggttta gctaagacat taacaaaagc tttatacgat agtaacataa caataggtgc
    20461 tctttttacc aatgcaccta actatcccag tgtaactata atcaatgagt tatcgaaact
    20521 gtacagagat cggggttgtg attcaatcat agctcttggg tcaggtggaa tagcacatat
    20581 tgcaaaagca gtcaatatgg ttgtttccct taaaactgat attttgcaat gtactgacct
    20641 tactatttca tcaccactga agccattgat ctgcgtaccc accgcagatt gtgaaggtgg
    20701 cgaaatggct gctgttgcat attatgagcg ttttgaaaaa caatcggact ttctctatcc
    20761 cgatgttatt gtgattgact cgcgcatcac acgccatgca acttcacaag aaatagcaaa
    20821 ctgtgcactc acatcactgg cacatgcaat tgaggcgtgt ggcattgttg cacccaatcc
    20881 tatcaatgat gcatatgcac atacagcact ttcatttatt tatcaatact ttccaaaaac
    20941 aattaaaaag ccctgttaca aagatggcag tgttgccata gttaatgcgg cagcctgtgc
    21001 tgacactgca ttttcaaatg caccacaggg aatagttcat acattagcgc aggcgatagc
    21061 tcttgatacc aatcttcagc ctggcattgt gatgggaatt ctgctcagat acagtttagc
    21121 tgccaaactg catgctaaag gtggtatacg ccctgaacta ttgttagctc ttgcaggcat
    21181 tgatacatat gcaaagactc ccgaaaagga ccgccagaaa gccgcctatg actatctcac
    21241 aaagtgtatt gatgcaacat gtaactatgt tccgtcaaca ttgaagggat tacatattcc
    21301 ccattacaaa ctgaaagaat atgccagatt agctgctgca aagaacaaac agcacaagga
    21361 aaatgaatat atggcactgt tagaaaaatc ctttgaaggc aaataaattt ttaaaaagga
    21421 gttacattat gaacgcatta caatatcctg aaattaagcc aaagaagaaa ttcattaaac
    21481 gaatatatat tgctgttatg ctgtgggtgg ttggccgagc cattcaggct gcagcaaaag
    21541 ttgacaggga agtaaaagaa gaatttgcaa aactaaaaga taatttttta ttgaagatgt
    21601 ggatatggcc aaatggacct gcaatgtatg ttggcaaaac taaagatggc aaggtaaaat
    21661 acctgggctg gaaagataaa ggctacaaac ccacattaac catgaccata aaaagtgttg
    21721 aggctgcaat gcttcttttt acattccagg aaggaactgc tatctcaact gctcgcaaca
    21781 ggctggttgt agatggggat ttgcctgatg cctgtgcact ggtgaggatt ctgaatatag
    21841 tagaagttta tttactgcca aaaattattg caaagcttgc agttaaacga taccccaaat
    21901 ggtcacagat gaacccctta agaaaacatt ttggcagaat acttatctat gttcgtgcat
    21961 ttacattcta aataaatgaa acgaggtgca atcatgcaat taccagcata ttatgaattt
    22021 tgtaacagtg ttaaaacaat agttggtcac aaggccctgg agcaaatacc ccagagcttg
    22081 aaagcaatca atgcacaaag accaatgatt attactgata aaggtgttga gcaggccgga
    22141 ctcataaaaa ttgtccaggc agcaatgcaa aaatcaaagc taaaagccgc tgcactcttt
    22201 gacagtgttc cggcagactc agatgtaaaa gtagtcaatg aagtggcaaa aatttatagg
    22261 gcaaaaaaat gtgattcaat tattgccgta ggtggtggct ctgttctgga tactgctaaa
    22321 ggggcaaata ttcttgtatc tgaaaatgca gctgatttaa tgcaatttgt aggttatggc
    22381 aatgttaaac gcaaactccg cccatttgta gcaatcccaa ctacagcagg tacaggttcc
    22441 gaagtgactt cagttgctgt cattgccaac catgaaaaga acatcaaaat ggcttttgga
    22501 tctctttttc ttctgcctga catcgcaatt attgattccc gcatgaccaa aacgttgcca
    22561 ccaaccataa cagctttcac tggaatggat gcccttaccc atgcagtgga agcatacttc
    22621 tgcctggcaa aaaatccatt aagcgacaca acagcgcttg cagcaataca gcttattgca
    22681 cagaatttag tcaatgtggt taaaaaacct tctgatcttg atggcaggtt agcactggca
    22741 ctggcttcaa acttagctgg catggcattt tccaactcaa tggttggcct tgtccacaat
    22801 attgggcatg ctattggtgg cgtgtgtggt gtaccgcatg gtgtatgcat gaatatactc
    22861 ctgccgtatg gattggagta caacatgcac aaacgttcac agtacattgg cgagttactc
    22921 tatccacttg ctggtgagga agtatacaac aaaacaccaa agaaagaacg accccttaaa
    22981 actgtagaat atatacgaaa acttattcag gatctgcacg atgccactga tggcaaacac
    23041 ccacgctttc tgaaagaggt atatgacaga actggcaagc agatgatacc caaagaaaaa
    23101 ttacctgata ttgccagaac agcaatgagc gaccccgcac aattttataa tcccgaagac
    23161 ttggattata atgattacat catggtttta gaagcagcat gggaaggaat accgcttgac
    23221 aggaaaaaaa taaaaaaagg ataatgtaaa aaacagtgcc gtgaattatt catggcactg
    23281 ttataagtac tttatgagga ttatgtatga aggttgaact ccattcatat aattcaaaaa
    23341 ttaaaaattt tacattgcaa tttatcctcc gcactgaagg aataagctac tttattatag
    23401 ttcccatact attacttttt ttatcaataa atattacttt tacatacaat caacttatat
    23461 tctttattat atgtattgct tttgcatttc caatttcatt cattactact caaataaaca
    23521 acattattgt gacaaagcca gtacagaagt attttaatgc agtagtaaaa aatgaagcca
    23581 taccagaaga atcatatgaa ttggcattta aaagatttct atcactaccc tatttgcaca
    23641 gcattggcgc ttttttcaga tgggtttttg gattatcgat gtttaccgtt cccataacac
    23701 tgtcacaaca attctcagcc acacaggtct ttataagctg gatgagtata ttaatatgtg
    23761 ctccgtcagg aactgtattg tatttcctcc ttactgaaat atttatccag gaaatttata
    23821 atacgggtat atttccaaaa atcccggaaa attttacatt caaaaacaca atgggtatta
    23881 caaaaaaact aatgatatca attaccatta ttgtccttac tccattcttt ataatgatag
    23941 catttatagc taccatactg gatcaaggag ctatacatct ttcttcttca tggtggaaac
    24001 ttttcatctt tggaagcata ggattactat tttctttatt tcttgcaaaa ttgttagcaa
    24061 aaacacttat tatgaagacc aacattatta aagaatttct gtttgctgtt gggaaagggc
    24121 agttagctgc atatacaaaa aagatagccg tttctgatga actggcggaa attaatatag
    24181 ccgtttataa tatgaaagaa aatcttagac gaatggttga gcttattcgc tcaagttctt
    24241 tagaacttaa agcaacaagc aataatatga aagtttcttc aacaaagttt tcagaagcat
    24301 cgcgcgacct cactgctatt attgaagaaa caagctcggc atatgaagag atgtcatcat
    24361 catttgaaat gatagtaaat actattaaat tacaacttga acaggcaaat acggtaaata
    24421 ccgaaataac cgttattaat tcttctgggg caaacttaac acatcaggta aatgaattaa
    24481 cgggaacttt taatgaagcc atcaatggag tggaaaaagg taaggaaaca atacacaggt
    24541 cagtaagcgc tataactgac atttctcaat acctggaaac agttgaatca acaatatcaa
    24601 caattaatga agtggcagat cagataaacc ttctggcact caacgcagct attgaagccg
    24661 cacgtgctgg ggaacatgga agggggtttg ctgttgttgc agatgaagtg aataaattgg
    24721 ctgatcagac agcatcttta gtcaagagta ttcaatcaac catagaacag tactccagta
    24781 aaattaaaaa tgaaataaaa tttataaatg aaacagctgc attatttgaa tcagttcgtg
    24841 ataaaatgct tgcaacgcaa aatgtattga atataaccca tcaattcaca aaagaactaa
    24901 ccacacaaaa tgaaagcatt atggggaaaa ttgaaaagtt agtacagctt tccaatgaga
    24961 tatacaatac ttccaatgag cagcaaataa caattgatga actgaccaaa gcaattaatt
    25021 ctattaatga agtagccacc catactgcag aagaatcttt tactatacaa tccttatcag
    25081 aaaaacttga agataatgcc aggcgactac tggaaaatat agaatatttt aaaatttcct
    25141 acgaataatt aattatttgc agacagtaca tagcctttta aggtgggact atttactact
    25201 ttacatttta ttcaaaaatc atctttgagc aatatatact aaaaaatttc caaaatccag
    25261 caattttcat tgacgatttg acattaattt agtattttat ataatactta atattataaa
    25321 aataacaaat actataacta tgaacacaaa atcaaacatt attgaagcta ttaatgtacg
    25381 gtatagcaat ttagcccatt ccagctgttg cctttcttgt ggtggagcta ttaatcatgc
    25441 ttatcctcaa ccaggtgaag tatgtgttga tttaggcagt ggacgtggca ccgatgtttt
    25501 gcgtatggct gatgcagtgg gtagcaatgg ttatgtttac ggaattgata tatccactgg
    25561 catgatagct aaatccaggg aactatcgga caaatttgga tataccaatg ttgcatttat
    25621 acaatcagac cttgaaacac tcccacttcc tgaaaacagc tgtgatgtta tcatttctaa
    25681 ctgcaccata aaccatgtaa acaacaaaca aaaggtattc aatgaaatat accgcatctt
    25741 aaaaccaggt ggaagatgca ttataagtga tatttattct attgggcagg tacctttaga
    25801 atataaaaat aatcctgatg cagttgctga gtgctgggca ggttcaattc ctaaagacga
    25861 gtacttagca attattgcac aggcgggatt ccctgagatt gtcatactgg aagaaagtga
    25921 accctatccc aaaggggcaa tttatgtagc aagttttacc tataaaatag taaaaccaca
    25981 ataaatggta tcacataatt aaagaggtgt taaatgaaat cacttgttgc accaaaagac
    26041 aacagtacca acgttgcaca gtgctgcgca aaaatttcta ctattgttgc aggctgccac
    26101 gactaaatca aacaccacct gtacgtttaa ccgtgcaggt ggcaaaaact tttgcaggtt
    26161 atatccaatg aaattatctc agtacactat tattcgtaaa ttacatgatt ccagcaatta
    26221 cttcatcatg aatcccctga gcatgcaggc tgacatcatt tcacagcaag agatggaagc
    26281 aattgttaac ggcacctatg acacaagcgt attgaaacag aaggggtata tgcttgatga
    26341 acagaaagag aagatgttat accgcaaagc gtatttagct tttcttgata cgcacgatag
    26401 ctctgaggtg caaatatttt ttgttccatg gtatgcttgc aattttgcct gctcatactg
    26461 ttatcaatca gggtatgaca acaaaggtac agtaacaccc gaaatcattg atgcattttt
    26521 ttcatatata accacacata tcacaaaacc aaagtacatt accctctttg gcggcgagcc
    26581 actgcttgat tcacctcatg cacggaaaat aatttcctat tttattgaaa aagcaaatca
    26641 gcattcatta catttggcga tagttaccaa cggatacacg ctgagttcat atattgacat
    26701 tttaaaaaaa gctactatcc gtgaaatcca ggtaacactt gatggtacag aagatgttca
    26761 caatgcacga agatttacaa aaaccaaaac accaacattt gatgcgatag ttacgggtat
    26821 agatactgca ctgaaacata acatacccat aaacttacgt gttgtcgttg atgcccaaaa
    26881 tattaatgac cttccaaact tagcacagtt tgccattgat aaaggatgga ccaacaatcc
    26941 attgtttaaa actcagatag gacgtaacta tgaacttcac tattgccagg ctgataatgc
    27001 aaaactttat gacagagcat cattgtatga aaaaatatat gaactcatta tacagcatcc
    27061 acacattctg caattccata agcctgcatt ctcaataagc aaatttttat ttgaacaggg
    27121 tgctatgcca aatcctttat ttgatgcatg ccctggatgc aagactgagt gggcatttga
    27181 ttatacaggg aaaatatatc catgcactgc aatggtaggc aaaaaaggcg aagaagttgg
    27241 tacatattac cccacagtgt cattgaacac tactgtcatt gaagaatggg aaaaacgtga
    27301 tgttacatcc ataccacaat gtcaacaatg ttctttgagt ttactgtgtg gcggtggttg
    27361 tgcagccgtt gcaaaaaata agcacggcaa tatactgcaa tctgattgca ggccagttga
    27421 tacattgctt tcaatgggtt tatcattata ttttgaaaaa gaaataggga attaccatgc
    27481 aaaatcatgt aataaccaaa aaagtatgta accagactgc cgaaagcggc aaggtatatc
    27541 aatactgaag gagtacatca tgagtgaaaa aatcaagtat attgatgcat cacaatttga
    27601 aaaggaagtt ctgcgtggtg gcaatgttat cgttgatttc tattctactg aatgtccacc
    27661 ctgtgaagca ttggctgcaa aatttgaacc tttgagtgaa atctttggtg atgacataac
    27721 atttataaag atattcaggc aggaaaatcg cgctttagct gaatcattag gtgtaacatc
    27781 atcacccact gtgttgtttt atcaaaatga cagggagata ggcactcgct tatcaggcag
    27841 cataaaacgc tctgagcttc ttgatactat taaactttta ttaccacagg agcgttattc
    27901 acagttacta tcggccatac aacctgtaac aacatacact gatattctta tcttaggtgc
    27961 aggccccgct ggattaactg ctggcattta tactgcacag gcaaaactta acacaattat
    28021 tgttgatact cagcttcctg gtggtcaggt ccagacaaca catatggttt caaactaccc
    28081 cggttttgca aaaccagttg aaggatttat gcttatgcac tatatgagtg aacaggcaaa
    28141 agaagcaggt tgccagttcc gtgttgctgt tgatgttaca gacataaatt taagaaagaa
    28201 atatattatc gtagatggta tagagactat tcatgctaaa aagattattg tagcttcagg
    28261 atgttcatac agagaactca atgtcccggg cgaatatgaa tataaaggga aaggcatatc
    28321 atactgtgca acctgtgatg ctaaatatta ccaggacaag catgtggtag tgataggtgg
    28381 tggtaattcc gcaattgagg aatcactgtt cattgctaaa tttgcacgtc atataactat
    28441 cgttcatcag tttgacaggc tgcaggcaaa cagggaagca caggaaaaag catttgccag
    28501 cgaaaagatt tctttcatgt tttctcatga accacgtgca ttcattaaaa atgatgatgg
    28561 cagcatgaca gtggaggttg aaaaccttaa aactggaaat agatcattca ttgcatgtga
    28621 tggtgtattc atatttgcag gcatgaagcc aaatatggtt aatgaagatt tgcaaaataa
    28681 caatggatat attattactg atgaattcat gcacacctca ttgcctgacg tgtttgcagc
    28741 aggtgatata cggtataaac catacaggca aataacaaca gcagtatctg atggtactat
    28801 tgctgcaatt actgctgtca gggaactgga agaagcaact gttgcggtat aatgattatt
    28861 tgacaggctt tgccacctgg taaagcctgt cctcaaaaac ttttacatca aatttttctt
    28921 tttcaaaatc atccggaaac aaatagagaa ttaaaagcca ttcaccttta actatatcaa
    28981 cattagcagg catggcatat acttttatat aatcatctgt tgcggtaatc ttaactctta
    29041 ctcgttcacc tttaatgatt ttaatatcat tgcgcaccgc atcctgtaat agaatatatt
    29101 caccttcata attttttaat ttcagttttt cttcttttgt tatgtaactg gaacattgcg
    29161 taagtactgc aatagtaaaa aagaataaaa caatacagct tgtgttaaca ataccaggta
    29221 agtatttatg tatgtgtttc attacgtgct ccttaacaga tagaatttac tataaaagtc
    29281 ataacaaata aacctttgac acaagttaaa atgaccactc tacgatcatt actgcaacta
    29341 acaacgaagg gctttgctcg tttctcagtt aaagggatct ttcgcattca gctcagaata
    29401 ctgttacaca ttcatttcat tctgagaccc aatgaaattg ggacgaagaa tcttgtcgtt
    29461 ttacacacag ccattactgc ataactgaaa ctgattccct tcgataaaca gttgctgagc
    29521 ctgccggagc atccaggaca agcgctttgc tcgcaatagc atttaactac cgttattgca
    29581 agttacaacg aagtgagggc ctggcaatct cattttgtca tccagaggag tcccccctta
    29641 taggggacga cgaaggatct ctcattttaa ttgagaatct tcctccgatt atgtcgttgc
    29701 cagaatgaca tcccatacat cgtcattgga agtcccggcg aggtcgtctc gatacgattg
    29761 cttgcgcaat cactcgacga ccacgtctcg a
//
LOCUS       DAORYL010000033        29516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1345_length_29516_cov_13.025220, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000033 DAORYL010000000
VERSION     DAORYL010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 29516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29516
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1345_length_29516_cov_13.025220"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..1911
                     /locus_tag="PLV81_07730"
     CDS             202..1911
                     /locus_tag="PLV81_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOM10027.1"
                     /translation="MKDPKKDQLCLEKYKELLPNLADYVEYYAKKKPKALAIIEHNTG
                     EKVTWKQFNTSVSAFAAKLLSIGLKKGDIVATSLPLLKEHIYLMYACYRIGVIIAPLD
                     LRLKTKEIQYCLDRMKPKAYFFLGKTPVADFRPMIAEVMKTSPYVQYWVQFQKEPEFI
                     MEGAIGVADFVKDIKKVFIKSLITGSVKRARKKVQKRDACLIIFTTGSTGSPKPALLC
                     HEGILVQNIGLAVGFELNENDRFLVNLPPSHVGCTTEQLATTIYGGGTSVILHIFDPK
                     LSLEAIQAHKVTCLGQIPALFNMEWLLPNYKDYDLSSLRFAIYGGQAVSREFLVKMKS
                     MAPRIGTGLGLTETSGFCTYTDVDADVDEIVQGIGYDMPLCPISIREPMNADGSAGRE
                     KSKGEIGEICFSGPQIFLGYLNDPDNTRKTISKEGICYTGDLGYYDEKGLHFAGRSKL
                     VIKPKGYQVYPDDVEHHISEKLKGKVNMVAVVGAEHNVYSEAIIAFVEPLQGVEITPE
                     DVMKACDDISAYSRPSHVEIVKPGQFPLNRVAKTDYVLLKEEAKKIVERLRSEGKWDK
                     AKQ"
     gene            2066..>2295
                     /locus_tag="PLV81_07735"
     CDS             2066..>2295
                     /locus_tag="PLV81_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10028.1"
                     /translation="MERADIWYCTDNDKGKEIFNALKILGLNITLIENRNFLSCNIVP
                     SRINIFIIDLINSDVSEILAMIRKDERIHKFL"
     assembly_gap    2296..2395
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2396..3099
                     /locus_tag="PLV81_07740"
     CDS             <2396..3099
                     /locus_tag="PLV81_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10029.1"
                     /translation="SEILAMIRKDERIHKFLKYLVLYKKQIRKASHLVSELMHLEILS
                     RPLQKREFSLLIEKSIIVERYKEMLSLFSHEAGERMETYANLLDINRKESFFDEKEKN
                     IFEKIIHFEKHIIDEQKKLNDAITNFAKIRQQELFDLKKRIDAEEMLAELRRKELLDA
                     QSIIEAQNAVIEYSSKELKDATEILHATEAVQELGRLEAMQLHEQLKKEKQKNEELLK
                     KVESLEKQIQELQSR"
     gene            3103..3660
                     /locus_tag="PLV81_07745"
     CDS             3103..3660
                     /locus_tag="PLV81_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10030.1"
                     /translation="MVYRLIGIVIILIISSSNFQSPVYAWFNRGLDDTLVEMHTMFNQ
                     RELVQLYDGYVHGDIKNDIMKLIDIMKMEKTDENKIALRLNYKNVEQYRKATSRDIII
                     RFFDLMKNPRYNQPSNIKNSAYLRIALSLSLLFAFLDNGMELVDRKIGFKCAILTYKG
                     NNFTMKIYFYYQNGKWFLTDGRQCQ"
     gene            3714..3980
                     /locus_tag="PLV81_07750"
     CDS             3714..3980
                     /locus_tag="PLV81_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10031.1"
                     /translation="MEWQKILADAVQDGKIKELHLKKIPVLKTCDNWREVEPIGWIDH
                     PMKLTHYKGALVKLRGNIYFVTEKTINALSEYINWKITQRIDVI"
     gene            complement(3981..5792)
                     /locus_tag="PLV81_07755"
     CDS             complement(3981..5792)
                     /locus_tag="PLV81_07755"
                     /inference="COORDINATES: protein motif:HMM:NF025011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HOM10032.1"
                     /translation="MRTLLQHIDTLEKKERYTLARMLGFQDPKKTTTKDIASLMSSHV
                     GLELIFSQCNPDELSILKLLSKNDVHFRDIEHNLHIPIYQIEKSAQLLASKSIVYILK
                     NRQLLSNKLDKICLYNELRQTLNPCDTHTLYEYCNEIIKHITSNKSHIPNKPHLSENA
                     SSFLKLCIQRGCVVSVNYAISTLGIIKANSVLNELTINKCIRIFHSISTHFSGIIMVH
                     PDYIQIKNESKNIHIHNHFMALIAMLKAYDCISSKGLFLTQEGNLRKSDVDNITKSLH
                     SVSIQTPGKPLTTTQMTQLTLYLLSITNSLSVTTTNVNTTLKHLHKMLLHPHQLTQMI
                     IHAAGKENNPLFPNPFPVSNDFIKNVLRTIKKYTNKTPDTYVLIYFLESLPEIFSDLL
                     HQIDDYYEIVRLNVLTAINFLCIMGVIEIINNTIQLSDIGKIITQQDKKSTQISVRAI
                     YINPDFSLVIPYHEIPSDISYILLAFTEITSTDVTIHAKISKIAILNAIKRGMNPETF
                     ISVLKEYAKNQLPQNIRFQLNEWIERTPSVIIKRGVILNSDKKEFIDELSHSLPKGAI
                     VERISDNHILINESAIDTVIKFAQKKDVVMTIQLVKE"
     gene            6015..7085
                     /gene="lepB"
                     /locus_tag="PLV81_07760"
     CDS             6015..7085
                     /gene="lepB"
                     /locus_tag="PLV81_07760"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOM10033.1"
                     /translation="MLQFLALNKQNTDTIQIQVLSNVQITKLMLKRIGYLILLIYSTI
                     FLAVAIPYSGTYAIGGFLCSVFMLRILNDFIKWRKFENATLTASPSGVSIQTKNENYH
                     FNAKDITYLEVNFFSNLVIRERYRSLGFPLMLVSNDDREKLIHYFYDMSPKRTVMFKK
                     IWEMFDAILVAFILAMHIRQFIIQAYYIPTGSMEDTLLVGDHLLAEKITYGPTIPQMI
                     GMKNPIHLSFLSIRPVQRGDIIIFRPPNEEEKDFIKRCIAVEGDEVHIDDGAVWVNGV
                     KLDEPYVKGVTSYRGFSEKRIEGVVPKGMIVAMGDNRENSYDSRGFGYVPLDRIKGKA
                     FILYWNTDNIKNLDFSRLGLIK"
     gene            7100..8923
                     /locus_tag="PLV81_07765"
     CDS             7100..8923
                     /locus_tag="PLV81_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOM10034.1"
                     /translation="MENVSHKRRIIITLIFISCIAVLLILKITQLHFSSRVIVNNKHD
                     IIQRGDIIDANGYPLAVSIYKYSIFADPRKVIDPDAVSLKLSSILAIPESEIKDLLTR
                     DKHFVWIKRKVDDNVINKVKELSLPGIYVKKEYARAYPEGTLASQVIGFVNIDNIGLE
                     GIEYKYNDVLLYADSSGEKEQKGLTVQLTLDRAVQYIAQKAINTYAEKVKPKKAVAVV
                     YEIKTGKIIALAVHPQFHPEYFYNYDKSVLTCFSVVDAFEPGSTMKIFAAITLLEHLP
                     DALKRTYICKGSVDVYDATIKCTKEHGKVSLNDIITYSCNAGIIQAMQPVKKEYLYQT
                     LTRFGFGEKTGIQLPGESQGILRNTKQWSGLSKYSMAIGQEISVTSVQLVAAFGAIAN
                     NGIYMYPQIVNQIKNHYGETVTKFFPKTKGKIVNETIVKTILSMMHNVVIEGTGKKAF
                     VAGYGTGGKTGTAQKSLPQGGYIPDAYVVSFVGIAPVDNPDICILVMFDEPAYGGSGG
                     ELAAPVFSDIAKRVLPLRGFGSVTVNAMKAKPLQYKTFKEDVLPDFIGQADTTALRKL
                     INLQHEYPIQYIMKGKGKVVAQDPKPGVPLKKVTTITLVLE"
     gene            8926..10779
                     /locus_tag="PLV81_07770"
     CDS             8926..10779
                     /locus_tag="PLV81_07770"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOM10035.1"
                     /translation="MNNIFQKNIMAIAGRNESLANIIRNTDYAPYIEVLQTKSGDAIP
                     VVKKESGHIAVHSKFDPVKEAQRLIDEYDCSQYDCVIVSGFGFGYHVEWLLQKVKKDA
                     TVLILEQFPWMIAAAIAHRDLQVILNDKRVILLVNPDEETIAIHMKGRSSYKTLFITH
                     RGSFQINPERYTNLQRIAKSYISTKEVNIATLAKFEKLWTSNIARNIREIISAPPAAA
                     FFDRFTNIPAIIVNAGPTLTQSIPYIQKHYNNTVIVAVDTSLKVLEYYGIEPHFCITV
                     DPQLINARYFEGVHTKRTVLIADPMVHPSTFLFYSGRKAITGVAFEMMKWIEQTIGSY
                     GNLRYGGSVSTNAYDFAKRLGAHPVIMVGQDLAFTQGLAHVKGAYLDDHIFFRNYRTN
                     TVEMFNRFQLTALPKIFVKGIRSRQVHTNQKMMIFISWFSRLNDPSLINATYDGAYIE
                     NVVHKTFEEITFQNSGLNIWNAIDEIYTHSVPDESAIALRSENLINQLSEMLQQTGTM
                     DAMLQQAIQFSEELIQNIKKRKTQSIASLVKKLDDIDKKLTELSVTKNLVGLTIQHII
                     HTVTEGYEIDESVTSEQEKVALKSLYLYKGMLEGLQYSTRVINNMIVLLQQ"
     gene            complement(10781..11536)
                     /locus_tag="PLV81_07775"
     CDS             complement(10781..11536)
                     /locus_tag="PLV81_07775"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOM10036.1"
                     /translation="MYPILFQYKFITIGGYGVMLAIGIYMAYILFERELILRNINPDL
                     AYKILLVAIPAGIIGSKVFHILDHMDEFLSDPAGTVFSGAGLSIYGGFLFALLFSYFL
                     IKKSNEPVLKILDITAPCIAVGYGFGRIGCHVAGDGCYGIETSSIFGVAYPNGIVPIS
                     YAVYPTPLFESFFSFIVAILLLQLRKKEFPQGTLFFIYLILNGLPRFFVEFIRRNPVV
                     FAGLTQAQIIALGLIMAGIAGLVIVHQKKLRNA"
     gene            complement(11751..13310)
                     /locus_tag="PLV81_07780"
     CDS             complement(11751..13310)
                     /locus_tag="PLV81_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-CoA synthetase"
                     /protein_id="HOM10037.1"
                     /translation="MANMYCRDTIADMVRRAALRFGEKTAIAFRDTSLTFRQLEEDCC
                     RFGHLMQQYGIEKGDRVAIIAFNSHYYPISFVGLSKIGAIQVPINYMLNGDEMAYIIN
                     HSKSKMVIVEDNLYNVIAPAKDKCTTVENWLYIPLEGTPTPQGFDNLIEMLQGMPTYD
                     IATTVYPEDIAQIPYTSGTESKPKGAMLSHRALLSQYFSCIIEGEYQPHDISLHALPL
                     FHCAQLHCFLMPFLYIGATNIILHKADPVSMMQLIEKYSITHMFSPPTVWIGIINHPE
                     FKKYNLTSLKKAAYGASIMPVEVLKRLTEVFPGLRLWNYYGQTEMGPVATILKPEDQL
                     KKPGSAGKPVLNVETALMDDDGNFVPVGEVGEIVHRSGHVMTGYLDDPEKTAEAFAYD
                     WFHSGDLGRFDEDGYLYVVDRKKDMIKTGGENVASREVEEVLYQHPAIAEVAVFGLPD
                     PKWIELVCAAVVLKKDMQATEEEIIAFAKQRLAGFKTPKKIFIMNALPKNPSGKILKR
                     ELRQTLAVETK"
     gene            13681..17202
                     /gene="nifJ"
                     /locus_tag="PLV81_07785"
     CDS             13681..17202
                     /gene="nifJ"
                     /locus_tag="PLV81_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOM10038.1"
                     /translation="MAKKIMKSMDGNEAAAYVSYAFTEVAAIYPITPSSPMAEHVDEW
                     AAHGKKNIFGQVVRVVEMQSEAGAAAAVHGSLQAGSLTTTYTASQGLLLMIPNMYKIS
                     AELLPGVFHVSARSISAQALSIFGDHQDINACRQTGFAMLASGGVQEVMDLAAVAHLS
                     AIKSRIPFLHFFDGFRTSHEIQKIEVLNYDDLAKLIDYAKLEEFRNNALNPEHPVTRG
                     TAQNPDIYFQAREVSNIFYQAVPDIVEEYMQEITKLTGREYHPFTYYGAPDAEYIIVA
                     MGSVTETIEETIDYLLKQGEKVGLVSCHLYRPFSPKYFFKVLPQTVKRIAVLDRTKEP
                     GSIGEPLNLDVRAAFYGTANPPVIVGGRYGLSSKDTTPAQILAVYNNLKQSEPKNGFT
                     IGIVDDVTFTSLPLPEDTSVVPEGTFEGKFFGVGSDGTVGANKNSIKIIGDNTDLYAQ
                     AYFAYDSKKSGGVTVSHLRFGKKPIRSTYLVNNPDFVACHVPAYLDKYDILKGLKKGG
                     TFLLNSLWDEEETKKRLPDHIKRYLAENNIKFYIINATEISERIGLPGRTNTIMQAAF
                     FKVSNVIPYEIAEKEMKGAIEKTYGRKGADVVSKNMAAVDEGAKNVKEVKVPEEWKNI
                     ELPGKPKFEELPEFIREIVEPMNRLEGDSLPVSAFLPRPDGTFPPGTTQYEKRGIAVN
                     VPEWQPDNCIQCNQCAYVCPHAVIRPFLLNEDEMKKAPADMVTLDAKGKGLEGLKFRI
                     QISPLDCTGCGNCAEECPAKEKALIMKPLETQLNQQKYWDFIVKNVTYKDNLMNKYTV
                     KGSQFCQPLFEFSGACAGCGETPYIKLITQLFGDRMLVANATGCSSIYGGSAPATPYC
                     INAQGRGPAWASSLFEDASEFGFGIHLGVAQQRNKLAQLLDQLAKTTSNSQLKDAALE
                     WLAGKDNAEKSREATKKLLPLLKGDSSKEAAEVLKLAQYLEKKSIWVFGGDGFAYDIG
                     FGGLDHVIASGEDVNILVLDTEVYSNTGGQSSKATPIAAQAKFAAAGKRVRKKDLGLM
                     AITYGYVYVAQVAMGASQMQYMRALTEAESYPGPSLIIAYSPCINHGLREGMGKAQEE
                     EKRAVECGYWSLWRYNPLLEKEGKNPFILDSKEPQWDKFKDFLMGEVRYSALKKEFPE
                     VAEQLFAAALDNAKWRYNNYKKLAAQS"
     gene            complement(17274..17438)
                     /locus_tag="PLV81_07790"
     CDS             complement(17274..17438)
                     /locus_tag="PLV81_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10039.1"
                     /translation="MINKINDVSTNTQGVLQKTKEMGKNTVEEARNRLSEVPKTLEEE
                     NKGKVINTQA"
     gene            complement(17465..18256)
                     /locus_tag="PLV81_07795"
     CDS             complement(17465..18256)
                     /locus_tag="PLV81_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HOM10040.1"
                     /translation="MIENLVHIILTNAAAVLAFFSIAWIISLVTRDASIADVFWGLGF
                     ILVSVLTFILYRGYDTRAIIIMTLVIVWGLRLSIYIFYRKRGKGEDPRYTAMRNKNPK
                     SFWIISLFKVFWLQGLLLIIISLSAQIGIAGQQPAKLLVTDFIGIVIWLTGFIFETVA
                     DIQLYVFIHNPANAGKVMNKGLWYYSRHPNYFGESLMWWGIFVIALPVPYGIATIISP
                     LLITYMLLKVSGVTLLESSLKEKRPEYVDYIKTTSAFIPLPKKKL"
     gene            complement(18318..20783)
                     /locus_tag="PLV81_07800"
     CDS             complement(18318..20783)
                     /locus_tag="PLV81_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOM10041.1"
                     /translation="MFPKPSDKIAIYIENQEISYNELIQNILSFASHLKIKKGDRVLI
                     FAENSPEWIYAFYSIWANKGIAVPCDYNLKENELAYILNNCTPKAVYCSANTLPTVKK
                     AIKLIKFKPSIYILEQCKVKPDVSLQLPEINYNDTAIIMYTSGTTGKPKGVMLTYDNL
                     LCSIQAIADLKMLTEHDRMIALLPFYHIFPLQGCVTAPLYLNASIVMVKELVLDKILA
                     ALQKYKVTMFLGIPRLYELLHKNIMAAVKKNPVAYALFLIAKLVNNLTFSKILFKKVH
                     NAFGGHIHAFLTGGAKLDPVVANDLKTLGFNLVEGYGLTETAPLVAFNPFSKVKLGSV
                     GLPVKGTEVKIVDGEILVKGRNVMKGYYKNKKATDEVITDGWFHTKDTGYFDKDGYLY
                     VTGRKDDMIVLPSGKNINPEEIEKHILRISPLIKEIGVIEDQGNLTAVIHPDYSVITK
                     ENIVNILETIKRKVINRYNASVASYKHITKITISKDELPKTRLGKLQRYIVKQLVQNE
                     KKSKTHEEIKEPKTIEYKSISRFLAIHTRKKVLPHHNLLVDLGLDSLGILSFQTYIEE
                     TFGIKISNADILNNPTVEELSTFIAHNKSKFEEHAQNWLQIIIKNTSYTFKHGFSLIL
                     SLKLLIKPLAKLYFQLKLNGKDNIPESPVIFAPNHQSFLDSLLLIASLPNKTLMHSYF
                     LSRELGFIKVSRGDSLAKLMNTIIVNIDTNLKDSIQKMASILQNKRNIIIFPEGTRTI
                     DGQPGTFKKTFAILSKELNCPVVPIAIRGAYQSFKRGKLLPKPTPITIDILPPVYPDK
                     LTVDNIVAKTQAAIAQTLKLENN"
     gene            21405..22763
                     /locus_tag="PLV81_07805"
     CDS             21405..22763
                     /locus_tag="PLV81_07805"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOM10042.1"
                     /translation="MVDVQSIVLRRKPSYPLSMWCEDYNSKKVTAEEAAKIVKSGDKV
                     FLGGGTGIPASFATALGKRAGTVKDVTIYQGYAMGLYDYMKPENRESFHIETMFVGPM
                     ERVCMEWKMAHYKPMHLSDIPQAGLDARCNRVAFTATPPNGDGFMNLSCFGAFLPRKE
                     VILNAETVIAEINSNTPWLNSVDFLVHVSEVDYIIEQDTPLFELPEIPITDVEKKMAE
                     IIADLIPDGATIQLGFGGLANAIGNLLYNKKDLGMHGEVVTPSVMRLVEAGVINGVKK
                     TFYPGKVIAAFAVGTREFYNYLDNNEKFLFCEVGWVNTPEIIALNDNLVSINNALMID
                     LTGQVAAESIGTKQYSGTGGQVNFIHGAKKSKGGKSIIALNSTYKDKEGKLHSNIVPY
                     LPLGTVVTTSRNDVEYVVTEYGVANLRMRSVYDRVLALINIAHPDFREELRYQAEKIG
                     WI"
     gene            complement(22812..23195)
                     /locus_tag="PLV81_07810"
     CDS             complement(22812..23195)
                     /locus_tag="PLV81_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOM10043.1"
                     /translation="MKKIISTENAPSAIGPYSQAVMEGGFLFISGQLGINPSTGKLEE
                     GIEAQTKRAMENIAAILSSAGITFDNVVKTTILLADINDFPRVNEVYESFFSNDNYPA
                     RATYAVAALPKGAFIEIEATAYVGS"
     gene            23409..24521
                     /locus_tag="PLV81_07815"
     CDS             23409..24521
                     /locus_tag="PLV81_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10044.1"
                     /translation="MEYTIVQYNKHRQLIIKEYSRKEFIAVCSDLLYRPKKIFVGLGD
                     FLEIGDYNQLLDAIFYDEESSIIENFLDEVNRKLSDKGQIPFSFLKLAVYYPDYLLKD
                     VPTQCVFTVGAKTRLFPGACHFMNFMKHYDPSILTAIPYELAIEIIRRVGLDDSHLVA
                     TEYKKIIGQKNIELYAGDIEQFISGERKIIEIEKYMAQTNTKEEDVLYVGRGESSVIT
                     FSKINSIAFNPSDAIIPLSHITVYGSSLESLLVLFNDSKEINKFLLSDIGEHILPSLI
                     VLSDVKEKPKEIIELEKKHRRLQANIIGQRIEHGTESYTTLQRDIELSLSAYPFNFQA
                     IKDMVRSRLEKFKINPKGFIDEICAIAQKRYQSWGM"
     gene            24577..25071
                     /locus_tag="PLV81_07820"
     CDS             24577..25071
                     /locus_tag="PLV81_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10045.1"
                     /translation="MAMYKNICVVMLMVVVLLFYWDNVFAQSVGYNKIFLGSAYNRVK
                     DILQHHDYNFKEMKCGVIRVILYDDSQSQGMLIFDYFDRLYTIIVTIPVTDAQFDQLQ
                     QHLQQKYGEFTVNDTVVCCTLGIYRIALHHNKTSQTATVEYGHTIPLFYPKKPVEENP
                     FSQF"
     gene            25092..26315
                     /locus_tag="PLV81_07825"
     CDS             25092..26315
                     /locus_tag="PLV81_07825"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOM10046.1"
                     /translation="MGNYRIYGFHLLFFLYLTQGIPFGFQVTALPVLFRQSGVSLTMI
                     GFSSLLALPWMLKIVWAPLVDRFWNATFGRRKSWLMPLQMVMFLSAYSATHIDIASNP
                     VLFTATIFVMNFCASIQDIAVDGLAIDILKDTDLGYGNAIQVVGYKIGMLISGGFLVW
                     LSSYLGWKNVFYVICGIIVIPVILLIPYKENIISQTKVNYITINDIYDHIKKAVKQHG
                     MIIVFLLIATYKTGETFIDGMFKPFLVDQGFTASQIGLWQGMYGMVASIAGSMIGGML
                     ASRYGIFNGLWFALLVRLIPLCGEWYCTLNVPSRELIIMVTIAEHFFGGLLTTAMFAF
                     MMYTVDKIIGATHYTILATIEVLGKSPGMWFSGLLADKFGYSYLFASGIILSIVPIVF
                     VSMVKNRLLYDEHTG"
     gene            26487..28460
                     /locus_tag="PLV81_07830"
     CDS             26487..28460
                     /locus_tag="PLV81_07830"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOM10047.1"
                     /translation="MYKKIICGIIIFCTIPSLYISCNQKRTPYKPKAIEGVLDLRSWD
                     ESSNQVISLDGDWEFYWNQLIEPSQFTKDLQPFTYIEVPKAWNKQGNFRYSFPAFGYA
                     TYRLHIYHDPKYVGQIKTLIMPYVHTAYTLWVNGKIISQNGKVGTSRSSMIPFQLPVI
                     TQFVINSTVTEVVLHISNFQQRTGGILRSIKYGNYNIINKQYELDLFITLFVTAALFI
                     ILLYHLGLFLVNKGYKPNLYFAFFCAIIGFRLLLTDEKLFVKIVPSLPWDMYLRMEYS
                     TLLFAVISFAHFIDGLYPRMFNTIILRAIDIYFCLFFIFVLVVPISYVSYALIFIQIG
                     LIVVGVYLLFIMIRATSTETTNSFIYISGNIILFFTLINDILYTQQLINSIYLFEIGL
                     LVFVFTQSLAALEESIKIQNRLIAINSELEIARRIQKTILPEAVPSTHSIHVEACYIP
                     MDEIGGDFYHFYELDEERLGIIIADVTGHGIPASLIASTVNIAFSLQRNHADNPATLL
                     ANMNTILYGKTGGQPITALYAYIDTSQKVVKFSRAGHPLPLIWRSSSNTITEIEIPGK
                     ILGVFKTIKNDITTYPIYSNDRIILYTDGITEIRNNRNEIFGIERFIACIEKNHHKST
                     RDLIDSIVDAVIQWAGSKNKITDDITLVIVEIK"
     gene            28685..28924
                     /locus_tag="PLV81_07835"
     CDS             28685..28924
                     /locus_tag="PLV81_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10048.1"
                     /translation="MSNHKHSTKQQYWEEYLYHYEASGMSKAAYCRENNLVYHQFVWW
                     HKRLCSQLKEGAEPFVALQHKLVIPTHRESGIVIE"
     gene            complement(29053..29505)
                     /locus_tag="PLV81_07840"
     CDS             complement(29053..29505)
                     /locus_tag="PLV81_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10049.1"
                     /translation="MNIETTTCISLHHLNLLSQYASSHKLSLHTFIINFINFVMSYKQ
                     IPVKACRRLQYRTRYHSWKRVHLYLLEHEYEFVMDVRKVCKMSLAKVIAYCVDNYLFD
                     FLAALDSDDNTDNYRCSGYTFSFYLEDGIQCCQFYWGPPPQLLKQATQ"
BASE COUNT         9653 a         4657 c         5694 g         9412 t
ORIGIN      
        1 tatacataat tttatatata atttattgct tgcaattaat ttgtatatat tatcatattg
       61 taatataaga tagcacaatt aaattttagg atatcataac attgtggagg aggattgtaa
      121 ccattatctg ggatagcata agccactgat tcagatgttg caatgacatt taagattaat
      181 tttataaagg gggaggttta tatgaaagat cctaaaaaag atcagttgtg tttggagaaa
      241 tataaagaat tgcttcctaa tttagctgat tatgtagagt actatgctaa aaagaagccc
      301 aaagcgctgg ctatcattga gcataatacg ggtgaaaagg ttacgtggaa gcaattcaat
      361 acctcggtat ccgcatttgc tgcaaaactt ctttcaattg ggttgaaaaa gggtgatata
      421 gtggcaacaa gtctgccact gttaaaagaa catatttacc tgatgtatgc gtgttacagg
      481 attggtgtaa taattgctcc acttgattta cgcctaaaaa ccaaagagat acaatactgt
      541 ttggacagga tgaaacccaa agcatatttc tttttaggaa aaactccagt ggcagatttc
      601 aggcctatga tagctgaagt aatgaaaaca tcaccgtatg tacagtactg ggttcagttc
      661 caaaaagagc ctgaatttat aatggaaggt gctatcggtg ttgctgattt tgtaaaggat
      721 atcaaaaaag tttttattaa aagtttaatt acaggaagtg taaaaagggc tcgaaagaag
      781 gttcaaaaaa gagatgcatg tttgataatt tttacaacgg gatcaacagg atctccaaaa
      841 cccgcattgc tgtgccatga aggcatactg gtgcagaata ttggtctggc agttggattt
      901 gagttaaatg aaaatgacag atttctggta aatctaccgc cttctcatgt tggctgtact
      961 accgagcagc tggcaacaac tatctatggt ggaggaacat ccgttatcct tcatattttt
     1021 gatcctaagc tgagtcttga agcaattcaa gctcataaag taacatgtct tgggcagatc
     1081 cctgcattat ttaacatgga atggttactg ccaaattata aggattacga tttatcaagt
     1141 ttacgttttg ctatttatgg tggccaggca gtgtccagag aattcttagt aaagatgaaa
     1201 tctatggcac ccaggattgg gacagggttg ggcttaaccg aaacgtcagg cttctgtacg
     1261 tataccgatg ttgatgcaga tgttgatgag attgttcagg gtattggtta cgacatgccg
     1321 ctgtgcccaa tcagcatacg tgagcccatg aatgctgatg gttctgctgg cagggaaaaa
     1381 tcaaaagggg aaattggtga aatatgcttc agcgggccac agatattttt aggttatctc
     1441 aatgatcctg ataacaccag aaagaccatt tcaaaagaag ggatatgcta tacaggggat
     1501 ttaggatact acgatgaaaa aggattgcat tttgctggca gatcaaaatt ggttataaaa
     1561 ccaaaagggt atcaggtata tcctgatgat gtagaacatc acataagtga aaaactaaaa
     1621 ggtaaggtca atatggttgc tgtggtaggt gctgagcata atgtatattc tgaggcaatc
     1681 attgcatttg ttgaaccatt gcagggcgta gagattactc ctgaagatgt tatgaaagcc
     1741 tgtgatgata tatcagctta ttccaggcct tctcatgttg agattgttaa gccagggcag
     1801 tttcctctca acagggttgc aaagactgac tatgtattac ttaaagaaga agcaaagaag
     1861 atagtagaaa ggttacgcag tgaaggaaaa tgggataagg ctaaacagta attaaagata
     1921 gagtaattac agttttataa aggcttactt taacggtagg cctttatttt ttttgattta
     1981 ataagaaaaa cggttgcaaa aaaattttgt ttgtatacta taaaaacaat atttaaacaa
     2041 attattttca tcaggatttc tcattatgga acgtgctgat atatggtatt gtacagataa
     2101 tgataaaggg aaagaaatct ttaatgcatt aaagatatta gggctaaata ttacactaat
     2161 tgaaaataga aactttttat catgcaatat tgtcccttcg cggataaata tattcattat
     2221 tgatcttatc aatagtgatg tttcggaaat tttggctatg atccgaaagg atgaacggat
     2281 tcataaattc ttgaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntttcg
     2401 gaaattttgg ctatgatccg aaaggatgaa cggattcata aattcttgaa atatcttgtg
     2461 ttatataaaa aacagattcg caaagcttca catttagtgt cagaattgat gcatctggaa
     2521 attttatccc gtccattaca aaaacgtgaa ttttctttat taattgaaaa atcgataatt
     2581 gttgaacggt ataaagaaat gttgagcctt ttttcgcatg aagcaggaga aaggatggaa
     2641 acatatgcca atttacttga tatcaacagg aaggagtcat tctttgatga aaaagaaaaa
     2701 aatatttttg aaaaaattat acattttgaa aaacatatta ttgatgagca aaaaaaactt
     2761 aatgatgcaa taacaaattt tgcaaaaatt cggcagcagg aattgtttga tttaaaaaaa
     2821 agaatagatg ctgaagaaat gcttgctgaa ttgcgaagga aggaattatt ggatgctcag
     2881 agtataatag aggcacagaa tgctgtaatt gaatattcaa gcaaagagct taaagatgca
     2941 actgaaatac tccatgctac tgaagcagtc caggaattag gaaggctgga agccatgcaa
     3001 ctgcatgagc agttaaaaaa agaaaaacaa aaaaatgaag agttgttaaa aaaagttgaa
     3061 tcactggaaa aacaaataca agagttacaa tcacggtaaa ttatggttta tcgtttaatt
     3121 ggtattgtaa taattttaat tatatcatca agcaattttc aatcaccagt ttatgcatgg
     3181 tttaacagag gtcttgatga tacgttagtg gaaatgcata caatgtttaa tcaaagggaa
     3241 ttagtgcaac tctatgatgg ttatgtacat ggtgatatta aaaatgatat aatgaaactc
     3301 attgatataa tgaaaatgga aaaaactgat gagaataaaa ttgcattgag gctgaattat
     3361 aaaaacgttg aacaatatcg caaagcaaca tccagggata tcattatacg attttttgat
     3421 ttaatgaaga atccgcgtta taaccagccg tcaaatatta aaaatagcgc ttatcttaga
     3481 attgcacttt cattatcgct tttatttgca tttttggata atgggatgga actggttgat
     3541 aggaaaattg gttttaaatg cgctatattg acctataagg gaaataattt taccatgaaa
     3601 atttattttt attaccaaaa tggcaaatgg ttcttgacag atggcaggca atgccagtaa
     3661 ggattgtaaa tattataaat agaattaaaa tacaatttat taaatagatt gttatggaat
     3721 ggcagaaaat attagctgat gcagtacagg atggaaaaat taaggaattg cacttaaaaa
     3781 aaattcctgt attgaaaaca tgtgataact ggcgagaggt ggagccaata ggttggattg
     3841 accatcccat gaaactcact cattataaag gggctctggt taaactgagg ggaaatatat
     3901 attttgtgac tgaaaaaact attaatgcat tgagtgaata tatcaactgg aaaattactc
     3961 agcgcattga tgtgatttag ttattcttta acaagctgaa ttgtcataac cacatctttc
     4021 ttttgtgcaa actttatgac ggtgtctatt gctgattcgt tgattagtat gtgattatcg
     4081 cttatcctct caactatcgc tcctttaggg aggctgtgcg atagctcatc aataaattcc
     4141 tttttatctg aattgagaat tactcctcgt ttaataatca ctgatggtgt acgttctatc
     4201 cactcattaa gctgaaatcg tatattttgt ggaagttgat tttttgcata ttctttcaat
     4261 actgaaataa aagtttcagg gttcatgcct ctcttgattg cattgagtat agctattttg
     4321 gaaatttttg catgaattgt gacatcggtt gatgtgattt cagtaaatgc cagaagaatg
     4381 tatgaaatat cagaaggtat ttcatggtac ggtataacaa gagaaaagtc cgggttaata
     4441 tatatggctc tgactgatat ctgggtggat tttttatcct gttgagtaat aattttgcca
     4501 atatcgctta actgaatagt attattgatg atttctatga cacccataat acagaggaaa
     4561 ttaatagcag taagtacatt aagtcggaca atttcataat aatcatcaat ttggtgcaac
     4621 aagtcagaga atatttccgg aagtgattct aaaaaatata taagtacata ggtatctggg
     4681 gttttgttag tatacttctt aatagtgcgt aaaacatttt ttatgaaatc attgctcaca
     4741 gggaagggat taggaaataa aggattattt tctttacctg ctgcatgtat aatcatctgt
     4801 gttaattgat gaggatgtaa taacatttta tgtaagtgtt tcagcgtagt gtttacattg
     4861 gttgttgtga cagataatga gtttgtgata gacaataagt ataatgtcaa ttgtgtcatt
     4921 tgagtcgtag ttaatggctt gcctggtgtt tggatagata cgctatgcaa tgattttgta
     4981 atgttatcaa catctgattt gcgtaaattt ccctcctgag ttaaaaacag tccctttgaa
     5041 gatatgcagt cataagcctt taacatagca ataagtgcca tgaaatgatt gtgaatatgt
     5101 atatttttac tctcattttt gatttgtata taatcggggt gaaccataat aatgcctgaa
     5161 aaatgtgtag atatactatg aaaaattctg atgcacttat taatagttaa ttcattcaat
     5221 acactattgg cttttataat accaagagtg gatattgcat agttcactga aacaacgcac
     5281 cctcgctgta tacaaagttt taaaaaactt gatgcatttt cacttagatg gggcttgttg
     5341 ggtatatgtg atttatttga tgtgatgtgt ttgattattt cgttacagta ttcatataat
     5401 gtatgggtat cacaaggatt cagtgtttgc cgcagttcat tgtataaaca tattttatct
     5461 aatttgttag atagtaactg tcgatttttc aggatgtaga caatggattt actggccaat
     5521 aactgagcac ttttttcaat ctgatatatg ggaatatgca ggttgtgctc aatatccctg
     5581 aaatgtacat cattttttga caaaagcttg agtattgaaa gttcatcagg attacattgt
     5641 gaaaaaatca gttcaaggcc aacatgactt gacatgagtg aagctatatc tttagttgtt
     5701 gtttttttag gatcctggaa gccaagcatt ctggcaagag tgtagcgctc ttttttttct
     5761 aatgtatcga tatgttgtaa taatgttctc attatttaaa aaatagtaga cacattaatc
     5821 gtgataatat acatgatatc ttcaataatt attccttgca ataattttat gatagatgaa
     5881 atgtgcatca ctgcttcaag tggtctggat gtattgtatt ccataattta ttcataaata
     5941 tttcaattaa aatgtaactt ttataaaaga attgcatata caacatatat ccttataaaa
     6001 gccgaggtac atagatgtta caattcttag ctcttaacaa acaaaatact gatacaattc
     6061 aaatacaggt tctaagcaac gttcagatta ccaagctcat gttgaagcga ataggatatc
     6121 tgatactttt aatttattca actatttttt tggcggtagc aataccgtat agtggcacat
     6181 acgctatagg tgggttttta tgtagcgtgt ttatgctccg aatacttaat gattttatta
     6241 aatggcgaaa atttgagaac gctacattaa ccgcatctcc atcgggagta agcattcaaa
     6301 ccaaaaatga gaactatcac tttaacgcaa aagatattac atacctggaa gtcaattttt
     6361 ttagcaacct ggtaatacgt gaacgttacc ggagtttagg attcccactc atgctggtaa
     6421 gcaatgacga cagggaaaaa cttatacatt acttttatga tatgtcgcct aaacgcactg
     6481 taatgtttaa aaaaatatgg gaaatgtttg atgccatcct tgttgcattt atccttgcca
     6541 tgcatatacg acagtttatt attcaggcat attatatccc aaccggttcc atggaagata
     6601 ccttacttgt tggggatcac cttttagcag aaaagataac gtatggacca actatccctc
     6661 agatgattgg aatgaaaaac ccaatacacc tttcttttct gagtatacgg cctgttcagc
     6721 gtggtgatat cattatattc cgtcctccta atgaagaaga aaaagatttt ataaagcggt
     6781 gtatagcggt agagggtgat gaagtgcata ttgatgacgg tgccgtatgg gttaatggtg
     6841 taaaattaga tgagccttat gtcaaaggtg tcacgtcata ccggggattc agcgaaaagc
     6901 gtattgaagg agttgttcct aaagggatga tagtagcaat gggcgataac agagaaaatt
     6961 catatgacag ccgtggtttt gggtatgtac cattagacag gataaaaggt aaagccttta
     7021 ttctatactg gaatacggat aacattaaaa atttagattt ttcaaggctt ggtttaatca
     7081 aataacattc aaggctgata tggagaatgt atcccataaa cgaagaataa ttattacatt
     7141 aatctttatc agttgtatag ccgtattgtt aatactaaaa attacacaac tgcatttcag
     7201 ttccagagta attgttaata ataaacatga tataatacag cgtggggata tcattgatgc
     7261 aaacggatat ccccttgctg tttcaatata taaatattct atatttgctg atccccgtaa
     7321 agttatcgat cctgatgctg tatcattgaa actttcatct atccttgcca ttcctgagtc
     7381 cgaaataaaa gatctattaa cccgtgacaa acattttgtg tggattaaac gaaaggtaga
     7441 tgataacgtg attaacaaag taaaagaatt atctttgccg ggtatttatg ttaaaaaaga
     7501 atatgcacgt gcatatcctg aaggcacatt agcatcgcag gttattggtt ttgtcaatat
     7561 tgacaacata gggcttgaag gtattgaata taaatataat gatgttttat tgtatgccga
     7621 tagttctgga gaaaaagaac aaaagggtct gacagtacag ctgacactgg acagagcagt
     7681 acagtatatt gctcagaaag caattaatac ctatgcagag aaggtaaaac ccaaaaaagc
     7741 cgttgctgtg gtttacgaaa ttaagacagg aaagatcata gcgcttgcag tacatccaca
     7801 gtttcatcct gaatattttt ataattatga taaatctgtg ttaacatgct tttctgttgt
     7861 tgatgcgttt gaacctggat caacaatgaa gatatttgct gctataacct tactggaaca
     7921 tctaccggat gcgctgaaaa gaacgtatat ctgcaaaggc agtgttgatg tatatgatgc
     7981 aactatcaag tgcacaaagg aacatggcaa agtttctctg aatgatatca ttacgtattc
     8041 atgtaatgca ggaatcattc aggcaatgca accggtaaaa aaagaatatt tatatcaaac
     8101 tcttacccgc tttggatttg gtgaaaaaac aggtatacag cttccaggtg aatcacaggg
     8161 tattcttcgt aatacaaagc aatggtctgg tctttcaaag tattcaatgg caatagggca
     8221 ggaaatatca gtgacctctg tgcaacttgt tgctgctttc ggagctatcg ctaacaatgg
     8281 catatatatg taccctcaga ttgtaaatca gatcaaaaac cattatggtg aaacagtaac
     8341 taaatttttc ccaaagacaa agggaaaaat agtgaatgaa acgatagtaa aaaccattct
     8401 ttctatgatg cataatgttg ttattgaggg aacaggtaaa aaagcatttg ttgcaggcta
     8461 tggcacaggg gggaaaaccg ggacagctca gaaatcattg ccacagggtg gttatattcc
     8521 tgatgcgtat gttgtatcat ttgtagggat agctccggtt gataatcctg atatttgtat
     8581 tcttgttatg tttgatgagc ctgcgtatgg cggttcaggt ggtgagctgg cagcacctgt
     8641 tttcagcgat attgcaaaac gggtattgcc actcagaggg tttggaagtg taactgtcaa
     8701 tgctatgaaa gctaaacctt tgcaatataa aacttttaag gaggatgttt tgccagactt
     8761 tattggtcaa gctgatacaa cagcattgcg aaagctcata aatttacagc acgaatatcc
     8821 gatacagtat ataatgaaag ggaagggcaa agttgttgct caagatccaa aaccgggtgt
     8881 acccttaaaa aaagttacta ctattacgct ggttctggag taagtgtgaa caatattttt
     8941 caaaaaaata ttatggcgat agccgggcgc aatgagtctt tagctaacat aattagaaat
     9001 acggattatg ctccatatat tgaagtactg cagacaaaaa gtggtgatgc cattcctgtt
     9061 gtaaagaaag aatcaggaca tattgcagtc cacagtaaat ttgatcctgt aaaagaagca
     9121 caacgcctga ttgatgaata tgactgttca cagtatgatt gtgtcattgt atcggggttt
     9181 ggatttggat atcatgttga gtggttgtta caaaaagtaa aaaaagatgc aacagtatta
     9241 attctggaac aatttccatg gatgattgca gcagctatcg cccacaggga tttacaggtc
     9301 atattgaatg ataagcgagt tatattgctg gtaaaccctg atgaagaaac gatagccatt
     9361 cacatgaagg gcaggtcatc atataaaaca ctgtttatca cgcatcgcgg ttcattccag
     9421 attaaccctg aacgctacac aaatcttcaa agaattgcaa aatcatatat ttccactaaa
     9481 gaagtcaata ttgccacact ggccaaattt gaaaaattat ggacttcaaa tattgcgcgc
     9541 aatatcaggg aaattatcag tgctccacct gccgcggcat tttttgatag gtttaccaat
     9601 attcctgcca taattgttaa tgcagggcca acattaactc aaagcatacc atatattcaa
     9661 aaacattaca ataatactgt tatagtggca gtggatacgt cccttaaagt tctggaatat
     9721 tatggcattg aaccgcattt ttgtattaca gtagatccac aattaataaa cgcacggtat
     9781 tttgaaggtg tacatactaa aagaacagta ttaatagctg atcccatggt acatccttcc
     9841 acatttttgt tttatagtgg caggaaggca atcaccggcg tagcatttga gatgatgaaa
     9901 tggatagaac aaacgatcgg cagctatgga aatcttcgtt atggcggttc agtatctacc
     9961 aatgcatatg attttgcaaa gcggttgggt gcccaccctg ttatcatggt tggccaggat
    10021 ttagctttta ctcaagggct ggcacatgtt aagggcgctt atttagatga ccatattttt
    10081 ttcaggaact atcgcaccaa tactgttgag atgtttaaca ggtttcagct tactgctctg
    10141 ccaaagattt ttgttaaagg tataagaagc aggcaggttc atactaatca aaagatgatg
    10201 atctttattt catggtttag cagattaaat gacccttcat tgattaatgc gacctatgat
    10261 ggtgcatata ttgaaaatgt tgttcataaa acttttgaag aaattacatt tcaaaattct
    10321 ggattaaata tctggaatgc aattgatgaa atatataccc attctgtgcc tgatgaaagt
    10381 gcaatagcgt tgcgttcaga aaacttgata aatcaattaa gcgaaatgct ccagcaaaca
    10441 ggcacaatgg atgccatgct gcaacaggca atacaatttt cagaagaact tatccaaaac
    10501 ataaaaaaga gaaaaactca atctatagct tcgctggtaa agaaactgga tgatattgat
    10561 aaaaagctta ctgaactcag tgttacaaag aatttggtag ggttaaccat acagcacatt
    10621 attcatacag ttactgaagg ctatgaaatt gatgaatctg ttaccagtga acaggaaaaa
    10681 gtagctttaa aatccctgta cttatataag gggatgcttg aagggctaca gtacagcacc
    10741 cgtgtaataa acaatatgat agtattatta caacaatagt ttatgcattc cgtaactttt
    10801 tttgatgaac aatcacaagc ccggcaatgc ctgccataat caaccctaat gcaattattt
    10861 gtgcttgtgt taagccggca aaaacaaccg ggtttctgcg aatgaattcc acaaaaaacc
    10921 gtggcaatcc gttcaaaatc agatatatga aaaaaagtgt tccctgtgga aattcttttt
    10981 tcctgagttg taataaaagt attgccacaa taaatgagaa aaatgattca aaaagaggtg
    11041 tggggtacac cgcgtaggat atcgggacaa tcccattggg ataagcaaca ccaaaaatgc
    11101 ttgatgtttc aataccataa catccgtcac cagcaacgtg gcatcctatt cttccaaaac
    11161 catacccaac tgctatacag ggtgctgtaa tatcaagaat ttttaaaaca ggctcattac
    11221 ttttcttaat gagaaaatag ctgaataaca gtgcaaagag aaatccacca tatatagaaa
    11281 ggccagcacc tgaaaatacc gtacctgcag gatctgataa aaattcatcc atatgatcca
    11341 gtatgtgaaa aacttttgaa ccaataatac cagctggaat tgctaccagt aatatcttgt
    11401 atgctaaatc cgggttgata ttgcgtaaga taagttcccg ttcaaacaaa atgtatgcca
    11461 tataaattcc tatagcaagc attacaccgt atccaccaat ggtaataaac ttatactgga
    11521 atagtattgg atacatggtg aagttcctcc ttttcattta attaatgcta ttttgacttt
    11581 atttaaagct ttctaaaaaa gatattatga tagtacatct attgaattat tttcagaact
    11641 gttgagtgta ctaaagcttt aatacaaaaa gtcatattgc ttacaacaaa aaaaattgca
    11701 acagaattat atcttctgtt gcaatttttt gtgctactac tgggtattgt ttactttgtt
    11761 tcaactgcta atgtctgacg taattcccgt tttaatattt tgcctgatgg atttttgggc
    11821 aatgcattca tgataaagat tttttttggc gttttgaacc ctgctaaacg ctgtttggca
    11881 aatgcaatga tctcttcctc tgttgcctgc atatctttct tcaataccac tgctgcgcat
    11941 acaagttcaa tccatttggg atcgggcagt ccaaatacag ctacttcagc aatggcagga
    12001 tgctggtata atacttcttc aacttcacgt gatgcaacat tttcgccgcc tgtttttatc
    12061 atatcttttt tacggtcaac aacatacaaa tatccgtctt catcaaatcg tcctaaatca
    12121 ccactatgaa accagtcata tgcaaatgct tcggcagttt tttcaggatc atccagatac
    12181 cctgtcataa catgaccaga tctgtgaact atctcaccta cttcaccaac tggtacaaag
    12241 ttgccatcat catccatcaa cgctgtttca acattgagta ctggtttgcc tgctgaaccg
    12301 ggctttttaa gctgatcctc tggttttagt attgttgcaa ctggccccat ttcggtttgc
    12361 ccatagtagt tccacaggcg caaacccggg aatacttcag ttaagcgttt taaaacctca
    12421 accggcataa tactggcgcc ataggcagct tttttaagtg aagtaagatt atattttttg
    12481 aattcaggat gattgataat tccgatccat actgtaggtg gcgaaaacat gtgtgtaatg
    12541 gaatactttt caatgagttg catcatggaa acagggtctg ctttatgtaa aatgatattt
    12601 gttgctccta tatacagaaa tggcatcagg aaacaatgaa gctgtgcaca gtgaaaaagt
    12661 ggcagtgcat gcaggcttat atcatgtggt tgatattccc cttcaataat acagctgaaa
    12721 tactgtgaca gtaaggctct gtgcgatagc attgcgcctt ttggttttga ttcagtgcca
    12781 ctggtataag gaatctgtgc aatgtcttct ggatataccg ttgtagcaat atcatatgtt
    12841 ggcatgccct gcaacatttc aataaggtta tcaaaaccct gtggtgttgg agtaccttca
    12901 agaggaatgt acaaccagtt ttcaactgta gtgcatttat cttttgcagg tgctataaca
    12961 ttatataaat tatcttcaac gattaccatc ttggatttag agtgatttat gatatatgcc
    13021 atctcatcgc cattgagcat gtagttaatg ggtacctgaa ttgccccaat ctttgaaaga
    13081 ccaacaaagc tgattggata ataatgtgaa ttaaacgcta tgatggcaac acgatcacct
    13141 ttttctatgc catactgttg catcagatga ccaaaacgac aacaatcttc ttctaattgt
    13201 ctaaaagtta aactggtgtc tctgaatgca attgctgttt tttcgccaaa acgcaaggct
    13261 gcgcgtcgca ccatgtcagc tattgtatct ctgcaataca tgttagccat aagaatcctc
    13321 catttatatt atgataagat atatgacaaa tatgaaatat accttctgtc gtgcaagcat
    13381 aaaatgttaa taaaatttaa aatagccaga gtatatatgt aatgaactat cgctcataat
    13441 gaatactttg aaaatatgca tataaatgct atgttaattg taatgatgta attttattaa
    13501 atatttagaa ttttattaat tgattttttt attgacttac agtacactaa agaaggaagg
    13561 ttatttacac gccgtgatct ttcattaatc aaaaattgtt attaagtaat taatctcagt
    13621 tttattactg agaagggatt tattatattt taaaaccaaa acagaaagga gaaagctttc
    13681 atggcaaaaa agattatgaa atcaatggat ggtaacgaag ctgcagcata tgtatcgtat
    13741 gcctttactg aagtagctgc catctatcca attacaccat cttcaccaat ggcagaacat
    13801 gttgatgaat gggctgctca tggtaagaaa aatatttttg gacaggttgt tcgtgttgta
    13861 gagatgcaat cagaagctgg ggctgctgct gcagtgcatg gatcactaca ggcaggttcg
    13921 cttaccacta cgtatacagc atcgcaggga ttgctgttaa tgattcccaa tatgtataaa
    13981 atatctgctg aattactacc gggtgtattt catgttagtg cacgttctat ttctgctcag
    14041 gccttatcaa tatttggtga tcatcaggat ataaatgcgt gccgtcaaac tggttttgcg
    14101 atgcttgcaa gcggtggtgt gcaggaagtt atggatttag ctgctgttgc tcatctttcg
    14161 gcaattaaat cacgtatacc atttttgcat ttctttgatg gtttcagaac ctctcatgaa
    14221 attcagaaaa ttgaagtatt aaactatgat gatttagcaa agctcattga ttatgccaag
    14281 ttggaagaat tcagaaacaa tgcactaaat cctgaacatc ccgttacacg tggtacagca
    14341 caaaacccgg atatttattt ccaggccagg gaagtcagca atatctttta tcaggcagta
    14401 cctgatattg ttgaagaata tatgcaggaa ataacaaaac ttactggcag ggaataccat
    14461 cccttcacct attatggtgc acctgatgct gaatacatta tcgttgctat gggctcagtc
    14521 acagaaacaa tagaagagac aatagattat ttactaaaac aaggtgaaaa agttggactt
    14581 gtttcatgcc atctgtacag gccattctca ccaaagtatt tcttcaaagt actaccacaa
    14641 acagtaaagc gtattgcagt gcttgataga accaaagaac ccggatccat tggtgaacca
    14701 ttaaatttag atgttcgtgc agcattttat ggaacagcca atccacctgt tatagttggt
    14761 ggacgatatg gattaagctc caaagatact acacctgcac agattcttgc agtgtataat
    14821 aatctcaaac agagtgaacc aaagaatggt tttactattg gcattgtgga tgatgtaacc
    14881 tttacatcat tgccactgcc tgaagatacc agtgtggttc cggaaggtac atttgagggt
    14941 aagttttttg gtgttggatc tgatggtact gttggtgcaa acaagaactc aatcaaaatt
    15001 attggtgaca acactgattt atatgcacag gcatactttg catacgattc aaagaaatca
    15061 ggcggcgtga cggtttccca ccttagattt ggcaaaaaac ctatacgatc aacatactta
    15121 gttaacaatc ctgattttgt agcctgccat gttcctgcat atctggataa atatgatatt
    15181 ttaaaaggtt tgaaaaaagg tggaacattt ttactcaatt cgttgtggga tgaggaagaa
    15241 accaaaaaga ggctcccgga tcatattaaa cgatatttag ccgaaaacaa catcaaattt
    15301 tatatcatta acgcaacgga aatatctgaa cgcattggcc ttccaggaag gaccaatacc
    15361 atcatgcagg cggccttttt caaggttagt aatgtcattc cctatgaaat agctgaaaaa
    15421 gaaatgaagg gtgccattga aaaaacttat ggcagaaaag gtgctgatgt tgtaagcaaa
    15481 aatatggcag cagttgatga aggtgcaaag aatgtaaaag aagtgaaagt tcctgaagag
    15541 tggaaaaata ttgaacttcc tggtaagcca aaatttgaag aattgcctga gtttattcgt
    15601 gagattgttg agccaatgaa caggttagag ggcgatagtt taccagtaag tgcctttttg
    15661 ccaagacctg atggtacatt ccctccaggt accacacagt atgaaaagcg aggtattgcc
    15721 gtcaatgttc ccgaatggca gcctgataat tgtattcagt gtaatcagtg cgcatatgtg
    15781 tgtccacatg cagtgatacg cccattcctg ctgaatgaag atgaaatgaa aaaagctcct
    15841 gccgatatgg taacgcttga tgcaaaaggc aaaggacttg aaggtcttaa attcagaatt
    15901 caaataagtc ctttagattg tacgggttgt ggaaactgtg ctgaagagtg tcctgcaaaa
    15961 gaaaaagcat taattatgaa accacttgaa acccagttga atcagcagaa atactgggac
    16021 tttatagtaa aaaatgttac ctataaagat aatttgatga ataaatacac tgtaaagggg
    16081 agccaattct gccagccatt gtttgagttc tctggcgcat gtgcaggttg tggtgaaact
    16141 ccttacatca agcttatcac acagcttttt ggtgacagga tgcttgtcgc taacgcaaca
    16201 ggctgttcat caatttatgg tggttcagct cctgctacac cgtattgtat caatgcacag
    16261 ggacgtggac ctgcatgggc aagttcactt tttgaagatg ccagcgaatt tggtttcgga
    16321 atacatttag gtgttgctca acagcgcaat aaacttgcac agcttttaga tcagctggca
    16381 aaaacaacaa gtaattccca gcttaaagat gcggcgcttg agtggcttgc aggtaaagac
    16441 aacgctgaaa aatcacgtga agcaactaag aaattattac cgcttttaaa gggcgatagc
    16501 tctaaagaag cagctgaagt gctgaaattg gcacagtact tagaaaagaa atcaatctgg
    16561 gtgtttggtg gtgatgggtt tgcatatgac attgggtttg gtggtctgga tcacgttatt
    16621 gcttcaggtg aagatgtaaa tatactggtg ttagacactg aagtatattc caataccggt
    16681 ggtcaatcat caaaagcaac accaatagca gcacaggcta aatttgctgc agctggcaaa
    16741 cgggtacgta aaaaagacct ggggctcatg gcaataacgt atggatatgt gtatgtagct
    16801 caggttgcca tgggtgcaag ccagatgcag tatatgcgcg cattaaccga agctgaaagc
    16861 taccctggtc catcacttat tattgcatat tcgccatgta ttaatcatgg attgcgcgaa
    16921 ggtatgggaa aagctcagga agaggaaaag agggcagttg aatgtggata ctggtcatta
    16981 tggcggtaca atccattact tgaaaaagaa gggaaaaatc catttatcct tgattccaaa
    17041 gaaccgcagt gggataaatt taaagatttc ctcatgggtg aggtacgcta ttcagcatta
    17101 aagaaggaat tccctgaagt tgcagaacaa ttatttgcag cagcgctgga taatgctaag
    17161 tggcggtata acaattacaa aaagctggca gcacagtcat aacattaact aaagataggg
    17221 gttgctgaaa cagcagcccc tttttaatat tttaatattg actacccctt cccttatgcc
    17281 tgagtattga tgaccttgcc tttattttct tcctcaagag tttttggcac ttcactcaat
    17341 ctgttgcggg cttcttcaac agtgtttttt cccatttctt tagttttttg taatacacct
    17401 tgagtatttg tggatacatc attaatttta tttatcataa accctccctt gttatatgta
    17461 cgtattataa tttcttcttt ggcaatggta taaatgcact tgtcgtttta atataatcaa
    17521 catattcagg gcgtttttct ttcagggaac tttcaagtaa cgtaacacct gaaactttca
    17581 aaagcatata ggttattaaa agaggtgata tgattgttgc aatcccataa ggtacaggaa
    17641 gagcaattac aaaaattccc caccacataa gtgattcgcc aaaataattt ggatggcgtg
    17701 aataatacca taagccctta ttcataactt tccctgcatt tgcagggtta tgaatgaata
    17761 cataaagctg aatgtcagca acggtttcaa atataaagcc agtgagccat attacaatac
    17821 caataaaatc agttactaac aatttggccg gttgttgtcc agctatgcca atttgagctg
    17881 atagtgaaat aataatgaga agcagaccct gtagccagaa caccttaaaa aggcttataa
    17941 tccagaagct tttggggttt ttattccgca ttgcggtata tcgtgggtct tcgcccttac
    18001 cacgttttcg gtaaaaaatg tatatagata gccgcaaccc ccatactata accagagtca
    18061 taataattat tgccctggta tcataacccc tgtatagtat gaatgtaagc actgatacca
    18121 gtataaagcc caatccccag aacacatcag caatgcttgc atctcttgtc acaaggctta
    18181 tgatccaggc aatggaaaaa aatgcaagga ctgctgcagc attagttagt attatatgca
    18241 ctaagttttc aatcataggt actatcgcct aagaatagga tacataaagt atatagtatg
    18301 agattatcaa agtcaaatta attattttca agttttagtg tttgtgcgat agcagcctgt
    18361 gtttttgcca caatattgtc aacagtgagt ttatcaggat atactggtgg cagtatatca
    18421 atagtaattg gtgtgggttt gggtagtaac ttaccacgtt tgaatgattg gtatgcaccc
    18481 cttatagcta taggtacaac agggcaattg agttctttac tcaggatggc aaatgttttt
    18541 ttaaaggtgc caggctgccc gtcaatagtg cgagtgcctt cagggaatat aattatgttg
    18601 cgtttattct ggaggatgga tgccattttc tgaatggaat ctttaaggtt tgtgtcaata
    18661 tttacaatta tggtattcat taattttgcc agagaatccc cgcgggatac tttgataaaa
    18721 cctaattcgc gggataaaaa gtagctgtgc attaatgttt tatttgggag agatgcaata
    18781 agaagtagtg aatccagaaa actctgatga ttgggagcaa agattacagg gctttcaggt
    18841 atattatctt ttccgtttag ttttaattga aaatataatt tagccagtgg ttttattaaa
    18901 agttttaaag acagtattaa ggaaaatcca tgcttaaagg tatatgaggt gtttttgata
    18961 attatttgta gccagttttg tgcatgttct tcaaattttg atttgttatg agcaataaaa
    19021 gtagacaatt cttcaacggt tggattgttg agaatatcgg cgtttgatat tttgatacca
    19081 aatgtttctt caatatatgt ctgaaaggaa agaattccta aggaatcaag gccaaggtca
    19141 actaaaagat tatgatgagg caatactttt ttgcgggtat gaattgccaa aaatctgcta
    19201 atggatttat attcaattgt tttaggttct ttaatttctt catgtgtctt tgattttttt
    19261 tcattctgta ccagttgttt tactatatat cgctgtagtt ttcctaatcg ggtttttggc
    19321 agttcatctt tggagatagt tatttttgta atgtgtttat aacttgcaac acttgcatta
    19381 tagcgattga tgactttacg ttttatggtt tcaaggatat ttacaatatt ttcttttgta
    19441 atcacgctgt aatcaggatg aataactgcc gtcagatttc cctggtcttc aataacccct
    19501 atttctttta ttaaagggga tatgcgtaaa atgtgttttt caatttcttc aggattaata
    19561 tttttgccac tgggcaatac aatcatatca tcttttctgc ctgttacata aaggtaacca
    19621 tctttatcaa aataaccagt atctttggta tgaaaccatc catcggtaat gacttcgtcg
    19681 gttgccttct tgtttttgta atagcctttc atgacatttc tgcctttaac cagaatttca
    19741 ccatcaacaa tttttacttc agtacctttt acaggtaaac caacagaacc caacttgact
    19801 tttgaaaaag ggttaaaggc aacaagtgga gctgtttcag ttaatccata tccttcaaca
    19861 agattaaatc ccagggtttt taagtcattg gcaactacag ggtctaattt agccccaccg
    19921 gtaagaaatg catgaatatg cccgccaaat gcattgtgga cttttttaaa aagtattttt
    19981 gaaaaggtta aattattaac aagttttgca attaaaaata atgcatacgc aacaggattc
    20041 ttttttacag cagccataat atttttatgt aacagttcat acaatctcgg tattcctaaa
    20101 aacatagtga ctttatactt ttgtaatgcc gccagtattt tatctagcac caattctttt
    20161 accataacta tagaagcatt gagatacagt ggtgcagtaa cacaaccctg aagaggaaat
    20221 atatgataaa aaggcaacaa tgcaatcata cggtcatgtt cggttaacat ctttaaatca
    20281 gcaattgcct gtatactgca taacaggttg tcataagtaa gcataacacc tttgggtttt
    20341 cctgttgttc ctgatgtata cataatgatt gcagtatcat tataattgat ttcaggaagt
    20401 tgcaatgata catctggttt gactttacat tgttctaata tatatatgct aggtttaaat
    20461 tttataagct ttatggcttt ttttacagta ggaagtgtat tcgcagaaca atataccgct
    20521 ttaggcgtac agttattgag tatataggca agctcatttt ctttaaggtt gtaatcacag
    20581 gggactgcta ttcctttgtt agcccatata ctgtaaaatg cataaatcca ttcaggactg
    20641 ttttcagcaa atatgaggac tctatcgcct tttttaattt ttaaatgtga agcaaaagat
    20701 aaaatgttct ggatcaattc attatatgaa atttcttgat tttcaatata tatagctatt
    20761 ttatcagaag gtttagggaa catcggttat tctccttcaa tgatgctgat acaaaaatag
    20821 aattaacata tacattcaac gatgtcatta tggatataat gtaataataa tttatttgga
    20881 tttgtacaaa attattggta ataattccta aaatgctttt aatgcaacgt aatgaaatat
    20941 aaagcgcttt accttttgct aaagtaatta atgaagattt tttcctgaaa aaagtttaaa
    21001 gaataaaaat ttaaaaatat aacaataata aatagaaatc ctgctactct tagtaagatg
    21061 atttataatt catgattaat aaaataattt aattataatt agtaatcaat tattatatta
    21121 attacaaatt tataatcagt tgtcaagtaa aaataaatgt ttataaaagt agataccagg
    21181 atagaatttt tgatttttct aaattaggca catataattt tgaaatgcaa taaccatgta
    21241 tttaaagtaa aaaataaata taggaaaata tataaaaagc ttgctatata tattataatt
    21301 ttaatataat aatatattat taataattat aaaatattat tttattgaaa aaatatccaa
    21361 tgtttacttt ttggctatca atatttttca ggaggtaatt gtatatggtt gatgtgcaat
    21421 ctatagtgtt acgtcgtaaa ccttcatatc cattatcaat gtggtgtgaa gattataata
    21481 gtaaaaaagt tacagctgaa gaagcagcaa aaattgttaa atccggtgat aaagtatttt
    21541 taggtggtgg aactggcatt cctgcgtcat ttgctactgc tttaggtaaa agggcaggta
    21601 cagttaaaga tgtgactatt tatcagggtt atgcaatggg tctgtatgat tatatgaaac
    21661 cagaaaacag agagagcttt catattgaga ctatgtttgt tggacctatg gaacgtgtgt
    21721 gcatggaatg gaaaatggca cactataaac ccatgcattt gagtgatatt ccacaggctg
    21781 gtcttgatgc acgttgcaat agagtagctt tcactgccac tccaccaaat ggagatggtt
    21841 ttatgaattt atcttgcttt ggggcttttt tacccaggaa ggaagtaata ttaaacgcag
    21901 aaacagttat tgcagaaatt aacagcaata ctccatggct taacagtgtg gatttccttg
    21961 tacatgtttc agaagtggat tatattattg aacaggatac accattattt gaattacctg
    22021 aaattcctat aacagatgtt gaaaaaaaga tggccgagat tattgccgat ttaatacctg
    22081 atggtgcaac aattcaattg ggttttggag gtttagcaaa tgccattggc aatttgttgt
    22141 acaacaagaa agatctggga atgcatggtg aggttgttac cccatcagta atgcgtttag
    22201 ttgaagcagg tgtcattaat ggagtaaaga aaacctttta tccgggaaaa gttatagccg
    22261 catttgctgt tggtaccagg gagttttata actatctgga caataatgaa aaatttcttt
    22321 tctgtgaagt aggatgggtt aatacgcctg aaataattgc attgaatgat aatttagtat
    22381 cgatcaataa tgcactcatg atagatttaa ctggtcaggt tgctgccgaa tcaattggta
    22441 caaagcaata tagtggaact ggtgggcagg taaattttat ccatggtgct aaaaaatcaa
    22501 aaggtggaaa aagtatcata gctctcaatt ccacatataa agataaagaa gggaaattac
    22561 atagtaatat tgttccttat ttgccattgg gcacagttgt tacaacctca cgtaatgatg
    22621 ttgagtatgt tgtgactgaa tatggtgttg caaatttaag gatgcgaagt gtgtatgaca
    22681 gggttttagc actgataaat attgcacatc ctgatttcag ggaagaatta cgatatcagg
    22741 cagaaaaaat tggttggata taatcagaag aataatagag aaaatacagc aagcttgtgt
    22801 gaatctaaaa cttatgaacc cacatacgct gttgcttcaa tttctataaa agcaccttta
    22861 ggtagagctg caacagcgta tgtggctctt gccgggtaat tatcattaga gaaaaacgat
    22921 tcatatactt cattaacacg agggaagtca ttaatatctg caagaagaat tgttgtttta
    22981 acaacattgt caaaagttat accagcgctt gaaagaattg cagcaatgtt ttccattgct
    23041 ctttttgttt gtgcttcgat accttcttca agtttaccag ttgaaggatt tatgcccaat
    23101 tgaccagaaa taaaaagaaa tcctccttcc attacagctt gagaatatgg accaatagct
    23161 gatggtgcat tttctgtaga aataattttt ttcatagggt attccttaaa tcttatttca
    23221 attcaatata atatgattgg tatatacttt taaaaaaaaa tataaatgtc aatttaaatt
    23281 caattctata ttttcattga catattgtta tgaaaaagtt tattcaaaaa agctttttaa
    23341 tttatgcttg ctcattttta tctaaggcac taatatgata gctcaaacag attttataag
    23401 agatagttat ggaatataca atagtccaat ataacaaaca tagacagcta attattaagg
    23461 aatatagccg taaagagttt attgctgtgt gttcagattt attataccgc ccaaaaaaga
    23521 tatttgttgg gttgggggat ttccttgaaa ttggtgatta taaccagttg ctggatgcta
    23581 ttttttatga tgaggaaagc tcaattattg aaaattttct tgatgaggtt aaccgtaaac
    23641 tttcagacaa aggtcaaatc ccattttcat ttttaaaatt agcagtatac taccctgatt
    23701 atcttttaaa agatgtgccc actcagtgtg tttttactgt gggtgcaaaa acccgtcttt
    23761 ttccaggtgc ctgccatttt atgaatttca tgaaacacta tgatccgtca atactgacag
    23821 caattcccta cgaactggct attgaaatta tcaggagagt aggtcttgat gatagccatc
    23881 tggtagccac agagtataaa aaaataattg gccaaaaaaa tattgagttg tatgcaggtg
    23941 atattgaaca atttatttca ggtgaaagaa aaattattga aattgaaaaa tatatggcac
    24001 aaacaaatac taaagaagaa gatgtgcttt atgttggaag aggtgaatca agtgtcatca
    24061 cattttccaa aattaattct attgcattta atccctcgga tgccataatt ccactatcac
    24121 atatcaccgt gtatggttca tccttagaat cattgcttgt attatttaat gattcaaaag
    24181 aaataaataa atttttgctg tctgatatcg gtgaacatat actgccatca cttattgtat
    24241 tatctgatgt aaaagaaaaa ccaaaagaaa ttattgagtt agaaaaaaaa catagacgat
    24301 tacaggcaaa tattattggg caacgaattg aacatggtac tgaaagttac acgacattgc
    24361 agcgtgatat tgagctatca ctgagtgcat atccttttaa tttccaggca ataaaggata
    24421 tggtgcgatc acggttggaa aaatttaaaa ttaatcctaa aggttttata gatgaaatat
    24481 gtgcgatagc tcaaaaaaga tatcaaagct ggggaatgta gaaaacgtaa cagtgtgtgc
    24541 ttgtgccgta caagctttat tggggaggga agttaaatgg caatgtataa aaatatatgt
    24601 gttgttatgt taatggtagt ggtattattg ttttattggg acaatgtttt tgctcaatct
    24661 gttggatata ataaaatatt tttaggatca gcatataaca gggtaaaaga tattttgcag
    24721 catcacgatt ataatttcaa agaaatgaaa tgtggtgtaa tcagggtaat actgtatgat
    24781 gattcacaat ctcaggggat gctaattttt gattattttg acaggttgta cactataatt
    24841 gttaccatac ctgttaccga tgcacaattt gatcaattgc aacagcattt acagcaaaaa
    24901 tatggagagt ttaccgtgaa tgatactgta gtatgctgta cccttggaat atatcgcata
    24961 gctctacatc acaataaaac ctcgcaaact gctactgttg aatatggcca taccataccg
    25021 ctattctatc ctaagaaacc agtagaagaa aatccttttt cacaatttta atttgtgttc
    25081 aggcaatgac cgtgggtaac tatcgcatat atggatttca tcttcttttc tttttgtatt
    25141 taacgcaggg tattcctttt ggttttcagg ttaccgcttt accggtcctg ttcaggcaat
    25201 ccggagtatc actgactatg attggattca gcagcctgct ggctttaccg tggatgctga
    25261 aaatagtatg ggcgcctctt gtagatagat tctggaatgc cacgtttgga agaaggaaat
    25321 catggcttat gccactgcaa atggtgatgt ttttgtcagc ctatagtgct actcatattg
    25381 acatagcttc aaatccagtg ttatttactg ctacaatatt tgttatgaac ttttgcgctt
    25441 caatacagga tattgctgtt gatggattgg caatagatat tttaaaggat actgatttgg
    25501 gatatggcaa tgccattcag gttgttggct ataaaattgg gatgcttata agtggtggtt
    25561 ttttagtttg gttgagttca taccttggtt ggaaaaatgt attttatgtc atatgtggga
    25621 ttattgtaat tcctgtaata ctgcttattc cttataaaga aaatattata tcgcaaacca
    25681 aagtcaacta cattacaatc aatgatattt atgatcatat taagaaagct gtaaaacaac
    25741 atggtatgat tattgtattc cttttgatag caacgtataa aacaggggaa acgttcattg
    25801 atggaatgtt taagccattt cttgttgacc aggggtttac tgcttcgcaa ataggcttgt
    25861 ggcagggaat gtatggcatg gtagcttcaa ttgctggatc aatgattggt ggtatgcttg
    25921 catcacgata tggaattttt aatggattat ggtttgcact gctggtacgt ttaataccgt
    25981 tatgtggtga atggtattgt acattgaatg taccgtcacg tgaacttatt ataatggtaa
    26041 ctatcgctga acattttttt ggtggtttgc taacaactgc aatgtttgca tttatgatgt
    26101 atactgttga taagattatt ggggctacac attatactat acttgcaact attgaagtac
    26161 ttggtaagtc acctgggatg tggttttccg ggctattggc tgataaattt ggttattcct
    26221 atctgtttgc gtcaggaata attctttcaa tagtaccaat agtatttgtt tcaatggtta
    26281 aaaatagatt attatacgat gaacacactg gttagtgtta atttgagaaa acactatcat
    26341 tgtattaatt aacaatcttc atactgtgtc tatgggacgg tatacatgta taatttttga
    26401 aaatgttctt tactttttta atttatatga aaatcttagt gaatattaat taatataact
    26461 attgaaaatt tgggttatta tatccaatgt ataaaaaaat tatttgtggt attattatat
    26521 tttgtactat accatcatta tatatcagct gtaatcaaaa aagaacccct tataaaccca
    26581 aggcaataga gggcgtttta gacctgcgga gctgggatga atcatcaaac caggttatat
    26641 cattagatgg ggattgggaa ttctactgga atcaacttat tgaaccttct cagtttacaa
    26701 aagatttaca accatttacc tatattgaag ttcctaaagc atggaataaa cagggaaatt
    26761 tcaggtattc atttccagct tttggatatg ctacctatag actgcatatt tatcatgatc
    26821 caaagtatgt agggcaaatt aaaacattaa ttatgccata tgtacatacg gcgtatactc
    26881 tatgggtaaa tgggaagatc attagccaga atggaaaagt tgggacttcc aggtcttcaa
    26941 tgataccttt tcagttgccg gttataacac aatttgtcat taattccaca gttactgaag
    27001 tcgttttgca tatatctaat ttccagcaac gtactggggg aattttacga agtatcaaat
    27061 atggaaatta taatataatc aataaacaat atgaattaga ccttttcatc acactgtttg
    27121 tcacagcagc cctttttatt attcttttat atcatcttgg tctattttta gtaaataaag
    27181 ggtacaaacc aaacctttat tttgcttttt tctgtgcaat tattggattc aggctattac
    27241 tgactgatga aaagcttttt gtaaaaatag tcccatcttt accatgggat atgtatttac
    27301 gaatggaata ctcaacttta ttgtttgcag taataagctt tgctcacttt attgatggcc
    27361 tatacccaag aatgtttaat accataattt taagggctat tgatatatat ttctgtttat
    27421 tttttatttt tgtattggtt gtacccatat catacgtttc gtatgctctt atttttatcc
    27481 agattggttt aatagtagtt ggtgtatatc ttttatttat aatgatacgg gcaacatcaa
    27541 ctgaaacaac aaactctttt atttatatat ccggtaacat catattattt tttacactta
    27601 tcaatgatat attatatacc caacagctga ttaatagtat ctatctgttt gagattggtt
    27661 tattagtctt tgtatttact caatcattgg ctgcgttgga agaatcaata aagattcaaa
    27721 atagattaat tgcgatcaac agtgagcttg aaattgcacg gcgcatacaa aaaactattt
    27781 taccagaagc agtgccatcc acacattcta ttcatgttga ggcatgttat atacccatgg
    27841 atgaaatagg tggcgatttt taccattttt atgaattaga tgaagaaagg ttggggatta
    27901 ttattgctga tgttaccggg catggtattc cggcatcttt gattgcatca acagttaata
    27961 ttgcattttc attacaaagg aatcatgccg ataatcctgc tacacttctg gctaatatga
    28021 ataccatatt atatggtaaa acaggtgggc aacccattac cgcactatat gcatatattg
    28081 atacatcaca aaaagtagta aaattctcac gggcaggaca tccacttcca ttaatctgga
    28141 ggagcagtag taatacgata actgagattg aaattcctgg aaaaatactg ggtgttttta
    28201 aaactataaa gaatgacatt accacatacc caatatatag caatgacaga ataatactgt
    28261 atactgatgg aattacagaa atccgtaata atagaaatga aatatttggc attgagcgtt
    28321 ttatagcatg tattgaaaaa aatcatcata aaagtacaag agatcttatt gatagcattg
    28381 ttgatgcagt tatacaatgg gcgggaagca agaataaaat aactgatgat attactttag
    28441 tcattgtaga aattaaataa agtcacttaa agctatctaa tggtaattgc atctatttcg
    28501 atgggagcac cagcaagcag atcaaagact caagatgcag gtatgcgcag gggaatttga
    28561 aggaaaaata atttttatat atttcattca ttgtatccgt caaattaaca ccaacttcac
    28621 aaaagaataa agatatgcat acactccgta tgtaaagcat acaatgagca tacggaagta
    28681 aacaatgagt aaccacaaac atagtacaaa gcaacagtac tgggaagagt atctgtatca
    28741 ctatgaagca agtggcatgt caaaggcagc gtattgcagg gaaaataatt tggtgtatca
    28801 tcaatttgtg tggtggcaca aacggttgtg ctctcaatta aaagaaggtg cagagccatt
    28861 tgtagcattg cagcacaagc ttgtaattcc cactcatcgt gagtcaggta tagtcataga
    28921 atgacggatg ctgcaagtaa aactaccagt ggaaagcagc agaaaaccgt gaagacagta
    28981 cttaaagcta cgtatccaat aggggatacg gttttcttct ttaggtgggg taataacagt
    29041 agtggtactg cgttattggg ttgcctgttt tagtaattga ggcggtggtc cccagtagaa
    29101 ttggcaacac tggatgccgt cttcaagata aaaggagaat gtatagccac tacaacgata
    29161 gttatcggta ttatcatcac tatccaatgc agcaaggaaa tcaaaaagat agttatcaac
    29221 acaataggca ataaccttag ctaaagacat cttacacacc ttccgcacat ccatcacaaa
    29281 ttcatactca tgctcaagca aatacagatg cacccgcttc cagctgtgat agcgagtacg
    29341 gtattgaagt cgtctacagg ctttaacggg aatctgttta tatgacatca caaagttaat
    29401 gaaattaatg atgaacgtat ggagcgatag tttatgggaa gaagcatact gtgataaaag
    29461 attaaggtgg tgaagagaaa tgcaggtagt cgtttctatg ttcatgaaaa cctcca
//
LOCUS       DAORYL010000034        29094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1382_length_29094_cov_13.129274, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000034 DAORYL010000000
VERSION     DAORYL010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 29094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29094
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1382_length_29094_cov_13.129274"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1533)
                     /locus_tag="PLV81_07845"
     CDS             complement(<1..1533)
                     /locus_tag="PLV81_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10050.1"
                     /translation="MDYIKLYRRFYLDMLVHGNALSGLIGAPLTVLFFIWKSEISFSN
                     LHIVIVPGFIVFIVVTLMTYYWYQFLFAPFRWFFTAKAKGASFSEKHALILKERLSSV
                     VVMFSLGLIAQWVIGLAIFSITSALFYGLSFDQLLYLWFGGILSIAISIIFGSYFIPK
                     TINRLTQEGLFDDVRDVISGKPVTAFGSLIPIITGSASIVNVLLVIMLVTFGIVLSHS
                     YMQKMFANNIDTIAKQTGHKLDSFFEYTKNDCSLIASFPDRNTINRIVTLRKIYTDCA
                     ILDNGNVILSGKGRYDGVFEKALGSLKTFEGSTSFITKPVFLGKEKRAYFATGDIING
                     KIYAALIPVDSFGKFILENAVIGKTGYIFITYNDGAGLVHPHRDMANDSLLRYDWGKK
                     LLENQNVFISYSSNNEDSFAYCTRSKLGFLLIASIPKAEMNEYSKLLALLSLGIGLFW
                     CAIATIILFRNIKRHLEPLKVAREVLSGVAQGNLTQSLAIIKGDEVGILSAAVNRLIS
                     ELR"
     gene            complement(1702..2511)
                     /locus_tag="PLV81_07850"
     CDS             complement(1702..2511)
                     /locus_tag="PLV81_07850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOM10051.1"
                     /translation="MSTKIAIITGAAGGIGRAITAEFIKKNITCVLVDINKKALADLQ
                     KQYGDKVYGLYCDITEIKSVKSTVNKAVKKYGAIDILVNNAGIIEPNLFENASYKEIM
                     RQIQVNLMGPINCTYEVLPYLKKSKSPSIVTIASLAGIVPETYSSLYTATKFALRGLF
                     LTLHIELKKSNIKVATVFPDSVNTPMLQYEATHGGSPLTFLSKPQKPEDVAKAVVRAI
                     ESKKPEIVVPSSQGFITRFIMVFPRLVVMVWPLLEKMGTKNKAIIQKTIKS"
     gene            complement(2573..3784)
                     /locus_tag="PLV81_07855"
     CDS             complement(2573..3784)
                     /locus_tag="PLV81_07855"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOM10052.1"
                     /translation="MKRKLWISISFVVTLLVFVYAQNPSRLKESGFNSAFTIPLETPY
                     EQYVELMTHVIANARVETNNKDAIVAMNSPFIFKPQVKPVNGIYPKGILLIHGLSDSP
                     YFLRAVGKYFTEKGFLVYGLLLPGHGTVPGDLLKVKNDEWIKATHYGMLQLKKNAKDV
                     YMGGFSTGALLSVHYVLSNNNHDVKGLFLFAPAFAIYSKLAWVAPIVGTFKDWESVED
                     DTDYTKYQSFTFNGVTQTYRLIKKVDELFDEGKRIQVPVFVAQSIDDRTVSTERTLFV
                     MKNYVTGNKMFVLYTTQPEKDYDGEDNFVITVNSYIPQEKILNIPHICITIPPDDPHY
                     GKNGDYRNCLYYKPNSPERQQCLSDPEIWKGENTEENIEKYKVITRLTYNPHFNSMCK
                     QMGEFLSVIEK"
     gene            complement(4050..4976)
                     /locus_tag="PLV81_07860"
     CDS             complement(4050..4976)
                     /locus_tag="PLV81_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10053.1"
                     /translation="MLLTRYSGKIIFSLLVFLLLSSPTLFAQTQIDLNPSAKTIDGIT
                     NIAASIVADSFVPMTHYNEDNYMVSIVPAYFTVQKVYDDPEINGNDVKGYGAGTGFGY
                     ATSDRMLIYGIAAYMHFDGRLSSAFLKGLDANIYQMKVDYSIISLYSGIGYDVVESSE
                     FSLPLFAGIFIQRYNGTGSLPEILNTKLSVDSSATLFGVSFGIAPSFKYNDLFKITPY
                     YLVSYSLNKPDATAKITTTVPFPITANYDVPANNIKASMVGMALTWLSSKSISLSLSL
                     GGWLRNETSWYNKTFLNGLQMKSAVLVVSFSN"
     gene            5536..7074
                     /locus_tag="PLV81_07865"
     CDS             5536..7074
                     /locus_tag="PLV81_07865"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOM10054.1"
                     /translation="MSILQKFLRIYDNKDYLTRQQAEALFILYFIVALGLAAMGVSMF
                     IIYKSYHPTHVGVLLCEIVLSISFFLNYKGKNIAAGYLMLFPLNIVLWYIIWIITGVD
                     DTITVTDSIFYVFPLIATATLINNRTSIIVFTIFNVLSHGAYTVYFHKTGVLNAKQFL
                     DLLQDGIIAISVFGGICVSFITMKMKSHALILNSAKEAQDNKSKIEEMLQQTYSVSKD
                     LAVSTSHLVGMTDNFSQATQSQASTLEEITSSVEEVTASGEGVLAMVQHQSGMGDYVK
                     NNMDSLYQIVTEVGEKIKEAIEIRNVLNDLVGQSRNEIQITLNDVHNALSQFHDMKNT
                     VEIIEDISDKINLLSLNAAIEAARAGEFGRGFAVVAEEIGKLADNTQANLKIINEMFN
                     KSNIGITQASKRLDEFIHLLNEMIGNIEKLSGSIDEVVKLARDDLNLNRQTRNSLEEL
                     LQEAGNILRATNEQKQALDEIAKSINVFNETIQDIASSAQNLSGFSVDLASMVQKLKS
                     MSGI"
     gene            7353..8636
                     /locus_tag="PLV81_07870"
     CDS             7353..8636
                     /locus_tag="PLV81_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HOM10055.1"
                     /translation="MQQQEYDAIIVGTGPGGATVARELSKKKKKVLMLERGSDPIIKG
                     SYVQAASMFMPGKGTLFTYGGLAMVRGITMGGSSIFYYATAFPPPVAMLKKYGIDITK
                     EIEECKKELPVAPLKDELIGPMAKRIMQSARELGYPWDKLPKFVFQDKCRPDCDKCNL
                     GCPYGAKFNARMWAEEAVTNGATLLVNAKVQRVLFDGKRAVGVEYTQGFKKHKVYGND
                     IIIAAGGIGSPVILRNSGIKGAGFDYFFDPLICVMGTVRDVKGGREFPMATGHQFVED
                     GYVMTDMTIPQLLYQAFTAEVLRFDRLFSHSHTLQIMIKEKDSLGGYLTDKEGVRKRL
                     VFDDKKKLMHGYERAKNILKNAGAKHIYKSWYVAAHPGGTVKIGHLLDKNLQTEKEHL
                     YVCDCSVIPEAWGLPPALTLYSLGKRLAKHLAKSK"
     gene            8675..9283
                     /locus_tag="PLV81_07875"
     CDS             8675..9283
                     /locus_tag="PLV81_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017744010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOM10056.1"
                     /translation="MPKVVNHDAYRDELLSGCFSVFARYGYDRVTMRKIAQVLGVSTG
                     TLYHYFPNKQAIIKALFDWVMRTNVGDALNRVRDNDNMDERIEITRQFWKEYGEYYQK
                     VMLLAFDLWRNSSVDENEHIFFEFAHTYKEAMVKLFDISYEFSEKLFLIFLGSVFHSI
                     LTPHHYSYANTVDKIVEIIKAEKGLDKQTGGKNKRTNIKLVR"
     gene            9327..10496
                     /locus_tag="PLV81_07880"
     CDS             9327..10496
                     /locus_tag="PLV81_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10057.1"
                     /translation="MKKNLYLSSVWMKLVATREGKNFLHQSIKEFFTQWANDSVLKKV
                     TAMVVLFCIKPFVRATPTDKTIQEILDDETWWQELSERLLHTIAEHDMAMAKHYADAV
                     STHAPDVTRKVAGDIWMYPAKVLTLFACIPSVANCTIRCANEALQPMNNQAPDLLADV
                     VNSLIKEIDSESLGCMANQLCELIRKFDAGDELIKESASTPFEQSVKTIANTVLEKLD
                     EKTKQQFFYSLLSLKNKITGGLLESLKDHPDILSMLINGIILSKLHNIQTARTVLSNY
                     RIQENMTDFPVEEIAHFCNDLLRLVVSVHATNKPLFDSLINRFAHALDTSAFQDFITQ
                     AGGDCFTALKPVLGYVFPFVLTCWTELIQEEDETMQQARKAFARALLKDVEADNV"
     gene            10489..11646
                     /locus_tag="PLV81_07885"
     CDS             10489..11646
                     /locus_tag="PLV81_07885"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOM10058.1"
                     /translation="MFEEFYTVAQTINNEYIVKAKEQGVQCIGYTCSYFPEEILHSLG
                     IIPYRVKGLNVSSLSVADAAFGPFICSHPKCLLQHFADGDYSFLDGIVVTPGCDSMRR
                     IDECIRKTAVNLNLPVVPPFFFHYAVPHKITEYSVKWLVDELQRFIDSVTEHFSLSFS
                     YEKLKESIAFYNRMRKRLEELNALRMNYPARIAGADAVAVYVAGLSMPRDRFYSMVES
                     LLSQYTGTNLEGRRRLMLIGSANDDIDFIKAIEGETAVVVADTLCYGPRLDVDYVDES
                     DEPLHALALRYLNHSHCPRMYGGYKQRLAKVVEAIERAQVEGVVLQNIRFCDLHGAEN
                     GLLERDLEKMGIPCLRLEREYGPMADYERMRMRIDAFIERLEVRRLVCDRP"
     gene            11631..13034
                     /locus_tag="PLV81_07890"
     CDS             11631..13034
                     /locus_tag="PLV81_07890"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOM10059.1"
                     /translation="MRQALKQYNFDWMLWRTYEAGSRLLAGTKQEYDIALRYIPYFRD
                     PVKEVFKAKAGQLFLQMTATYLDNIVHAHQRGKKTCMTTFCFSPAIVYALDMVPVTLE
                     LLTVMMTFLYKRGTGEFLDYCNEAGFTETSCSSQRGSMGALLADIGTPIDMVVTDSPG
                     VCDTNANAFSFFAAYKDLPFFQLNMPPWLVEERVTEYHVQDYKALVAFLEEQSGKKLD
                     IDRLRQILTELQKQDELINELEQFLCYRPLPLPVTTCLFIYAGRFMFAGMPIYTRLLE
                     LLVNEGRENVRLHQQGIYTSHEKSRGFFCYIDHYTHSLRLWQMLQELNIGYSGNILSH
                     FWADSNPPVIQNNWEEAAYSIKTGTLEDMLTSIAQINSRMPMIKSIRGPYDSPYMWLQ
                     DTLALASMYRADFIVYNGTPGCRNTWGMVKLLAQDTEKAGFPTHIMYADAFDDRVQSW
                     DATRDRFEEFLRVRRLI"
     gene            13031..13816
                     /locus_tag="PLV81_07895"
     CDS             13031..13816
                     /locus_tag="PLV81_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOM10060.1"
                     /translation="MIVAGCDIGSLTTKVVLFDGKKILASSIIKSKPKPEDGAYEALQ
                     QACAIATVNQDTITYCVGTGYGRKRVPFANDTISEISCHTKGAFYLYPNVRTIIDIGG
                     QDCKVISLNTQGEVDRFVTNDKCAAGTGRFLEVMAKVLKVELQELSNMAKKGNEAITL
                     ASACTVWAQADVIKYLNEGISKENIALGVCKSMAKRVGVLVNQASIKKDVCITGGVAK
                     NTAVVKELENLLGVTIIKITNVDPQLTGALGAALFAYQKAGGK"
     gene            13816..14604
                     /locus_tag="PLV81_07900"
     CDS             13816..14604
                     /locus_tag="PLV81_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOM10061.1"
                     /translation="MIVAGCDIGSTTAKAVILDDSTIIATILLPVEGSPKDAALLLLS
                     SVQEKTGIQPSQIEAWCGTGYGQKNIPYASTVESEIVCHAKGVQFIDPDVRTIIDIGG
                     QDAKVCRIDAYGNVQRYVYNDKCASGTGRFLEITADVLEVPITELATLDAKGVKEVTL
                     SNQCVVFAETEVISLLNSGEDMGNIVKGLHRALASRVASLALSIGVEEKIAFTGGVAH
                     NAGMVGALERVLKTKLVVHEYCQMMGALGAALIAQERIKNRVAS"
     gene            14591..15262
                     /locus_tag="PLV81_07905"
     CDS             14591..15262
                     /locus_tag="PLV81_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10062.1"
                     /translation="MLHPDIKNVLEYVQNHKKGTIYSVKGKEPWPEVLHAAMIFKDDI
                     GCELGGPFGSLALHCMYDEVVDGRVIVIGSDMLQKQSVWGRLTLIDAAVDDEESYYSF
                     LQQVTLIPVRHSMEGVMIRIQPSEYKEWIRIHKDYIKTHTVIQLFSRLYTWYKQLPWV
                     RAVEFVAITSCSDDIALLLPLIEKHKSIIKAFEKRLSEDIKNCNACDNNAVCSTINSL
                     FEKGP"
     gene            15265..16008
                     /locus_tag="PLV81_07910"
     CDS             15265..16008
                     /locus_tag="PLV81_07910"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOM10063.1"
                     /translation="MDEAKTKVIAFCGKGGAGKTSLCALTIKYLAHSKKRVLAIDADP
                     AGGLSWALGLKPHKTLDDLRLSLHEAVGTKAVQSTDELYAMANYELLASLVEHAGFDF
                     LSVGRPEAEGCFCSINAFLRHCIGKLTEHYEYVCIDGEAGLEQVNRRVLETVTHLIIV
                     SDTSKRSIEIARNLFELARQRTKTMHCGIIINKMGDSSCQIDQLPVWGMLPVSRAIAV
                     YDSEGRSLLELSDNEEYYTMFKAILKKYM"
     gene            16341..19010
                     /locus_tag="PLV81_07915"
     CDS             16341..19010
                     /locus_tag="PLV81_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOM10064.1"
                     /translation="MNTHHFENSFDILDYFNDAVFIITYDGSIVYANKTAYDRLGYSK
                     EELLQKNIRDIDSPTYARLIPERIEQFKQRGSLVFESEQVTKDGSIIPVEVSIHSILY
                     NSTPCIISVVRDITSRKQAEKELRESEEKWRAITENLTDIVWIVNLQFETIYINKAVE
                     KLFGFTVDEYLQRKVQEKYPPEVLQDIYNKLIEEFENEKKPGIDKNRTRIVEIQEYKK
                     DGTLADLSVHVKFLRDEAGNPNAIIGISRDITEQKKRLKEIQRYKLIFEQSINEIYIF
                     DAKTLMFTYVNQASINNLGYSLEELQRMTPVDIKPEFTLQQFNELLSPLREGTEKSIV
                     FETVHKRKDGSTYAVEVHLQLVKIDGEEHFAAIILDISERKRIEKSLKESEEKFRILA
                     ESTPMTIMMYQDNKWIYANPAAEALLEYSEQELCSMNFWDVVHPDDKAIVIERGSKRQ
                     KGEDATVGYEFRVISKSGMIKWVLLYGATVMYKGKPAGLISVLDITERKKIEEEKRKL
                     QEQLLQSQKMESIGTLAGGVAHEFNNMLNIISGHAELALLNIPQENDARKDIEEIKKA
                     AQRSAEITKKLLTFARKQEEKSSIVNVNSAIDSMIGMLKRLIGENIQLVWQPANRELS
                     VQIDEVSIDQILVNLSVNARDAIDKQGTITISTHSVSIKDEVALQHNVLPGEYALIQV
                     TDDGIGIDEETQKLIFDPFFTTKEVGKGTGLGLSTVYGIVKNNGGFIEVQSEKEKGTT
                     FKIYLPVFIERRKFAQYHDNAVKDLFHGTETILIVEDEVSILKMIKKMLEILGYTVLE
                     AGDPFEGLEIVKNYIGTIHLLITDVVMPKMNGVDLAKLVSAIYPQIKILYTSGYSAEV
                     LLKHNIKASDINYLSKPVSFASLASTVRTILDN"
     gene            complement(19149..20087)
                     /locus_tag="PLV81_07920"
     CDS             complement(19149..20087)
                     /locus_tag="PLV81_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HOM10065.1"
                     /translation="MQFPKPVICVSACLNGQNIRYNGQTINDDFVRLLLKYCKALDVC
                     PEVSIGLGVPRDHILVYYANNQFRLFQPATHKDVTGLMQQFATSFLSTLQGVDGFILK
                     AKSPSCGVSGTKIYKDTMGSIFHSKGKGLFATEVINKFGFLPIEDESRLRDEDIRENF
                     LIRIFTLARFREMLSNNVSIQKLIQFHQENKYIMMTYYQRKLSQLGRIVAGYKKGAFG
                     KTLNDYKTVFYTAFTRKPSQKQHVNTILHIFGYFSDKLNSNEKKHFLHLLDQYREHKI
                     SRIVIIELLRNWAHRFNDTYLLSQQYLDPYPEELKT"
     gene            20213..21751
                     /locus_tag="PLV81_07925"
     CDS             20213..21751
                     /locus_tag="PLV81_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOM10066.1"
                     /translation="MKVKSIADLYALYNPLIRRRAVLLYIMNIIILCLVLPVPFLVLI
                     IRGDFLRPFIIGIAPIIGSLISIITLRKGIYHFSANATSLATSIAILLGSYLQYQGTP
                     GIGYSSMIYIMPAGIVFASLFCSRLWTTGIATLFFISNIYFYYLNTLQGIIDKKILFT
                     GLIDSSLSIVFCFALSFLIVKIMKDSMNEIQIESDKNKEQYAQLKELIIAVQQVAANL
                     KDASAKMRDMTGRLSEQAQSQAASVEEVTSAIEEVNASMDLVNQHVKDQTVNLELLDS
                     MTSDFTELFGQLRQTIDTVARKIYTTADQSRNNEKTLEELNITMQEVGVKSQQMNSVA
                     DVIDTIAEQISLLALNASIEAARAGEAGRGFAVVADEISKLSDQTGESLKEIYNLTKE
                     TETQIQKGIKIVQETVQAMSAVIHNVHEISNDIQNISKNMAMQIENNKTISDKTKETH
                     QHADEIHYSASEQMTALGEVAKSIAMINDYTQDLSNAVVNLLEIAKIVDNEALQLKER
                     TQIL"
     gene            complement(21765..23780)
                     /locus_tag="PLV81_07930"
     CDS             complement(21765..23780)
                     /locus_tag="PLV81_07930"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOM10067.1"
                     /translation="MTKNNTKIELLAPAGSPEALVSALDSGADAVYLGLKDFNARKRA
                     KNFTHDEFKYAVNYVHHHNKKIYLTLNTLLYDHELEQVAEILNLAMEYAVDAIIVQDI
                     GLIQLIRTYYPSLTIHASTQTFCHNSMHARFLKNLGVERIILPRELSLHEIKAIQQKV
                     PCEYEVFIHGAMCFSFSGCCLFSSHLFSQSGNRGRCLQPCRYPFIIDGTTRYPFSMKD
                     LALGPSLADYINAGITKFKIEGRLKSTWYIKEVVSYYRKLIDSLMEGKPFKKEAPGLR
                     STTKGYMFDSSYDALVDATTLGVAGTYIGTVTQVKNKSCIISTRHPLQKGLRLRIVDN
                     SGKKVYEGTLLDYTYDKNKHSLEWYISIKSKQPLSVYIIGESKSDNTLKKLKAIPYKP
                     YSVQIHITIDSEFNISACIDTFTVQYTYTIPIQKAITQELSPNTIQTIFSQTAHFPFI
                     ATVTVTINDTIFIPLSQLKQLRRKFYEELYNVYIKHHESINQQRKSDIIAHIAIIKKN
                     NTAIQSSNTAYTWQHFEKLQTTDANNTFIELPVFVPEDELDDTIKKIENYMQSGFKHF
                     IIPTYGWLEFFKGNNVVLCAGDFCYCFNSFTYEFYKNNSVSCFTISGDMDALDITTKY
                     YNGYIQFKKPRIYLVTRLRIPDAVYTFKHKQFCPQHYANYDVLIDCE"
     gene            complement(23904..24401)
                     /locus_tag="PLV81_07935"
     CDS             complement(23904..24401)
                     /locus_tag="PLV81_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10068.1"
                     /translation="MYFETTTCISLQHIAILEEYAQKLGISLRTFIVYMIMYAAKKEK
                     RKYKAFKRIAYRKRNTDNPWKRVHLVLYQSEYEFFLDVKKLWKMSLAHIIEFCADNVL
                     VEFFEYFKVRLAKINSDNYPENLPSYYENRSYTFDFYRENGIHCLKFYWGPPPELFNS
                     LSAIN"
     gene            24717..25304
                     /locus_tag="PLV81_07940"
     CDS             24717..25304
                     /locus_tag="PLV81_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOM10069.1"
                     /translation="MKNAQSFRELKESEKENRKKIIIDAAERVFATKPFNQVTMRDIA
                     KEAGITAGAIYRYFPDQQSLFVEAFLRGAEKIINDLEQVISRSKNPLKDTIATFLKFL
                     MKHDQYFRMMTHFMLDAHLNQDLVEKLNEAERRLLDQFTKLFEQRSHARLLAHSLFAA
                     MNGVVITFHNYPGRSKKDIHQHMNRLGDMLYQLFS"
     gene            complement(25293..26894)
                     /locus_tag="PLV81_07945"
     CDS             complement(25293..26894)
                     /locus_tag="PLV81_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOM10070.1"
                     /translation="MALNTSYNFDEYLEWRKNVDYYADDPFLQKVTYYFAKDDFEKVD
                     QAARKLSKLASYRWRDIADTIAQPHNRIFMQHYDGHNHRIDRIVRPKELEEMEKEVFG
                     QGLFSAKTLPWERLVKLILIYENGEAGIACPLVCTEGLVELLEKYHDHPKLEKILQHC
                     KEGIDGEFAIGAQYLSEIQGGSDVAANDLEAVDNGDHWLLYGKKFFCSATHADYVVIT
                     ARPKGSEHVGLFVMPSWSDKTNEKRNGYTIDRLKWKLGTAELPTAEITFNGAIAYKAG
                     PIERGIANVVGIVLTHSRLTVGLSAAAAMIRASREATQYAQFRNAFGFPINQFPLLKR
                     QLDEINHYAQRTLAGAFKIYEMFQSLPGGLKGGLVTDEDVEIQRKRFRVRILIMLQKI
                     VASWDATDILRKAISVFGGHGVMEDFSSLPRLFRDMMINELWEGPRNVLLTQIYRDIQ
                     RASSWYPVHDFIHDLIYNANSKSINEIAASFAECLQSNFIYPGQENDHLCIMWEHACH
                     NLLHEFQNCALHEVEDAQMAISLRK"
     assembly_gap    27234..27333
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            27542..28378
                     /locus_tag="PLV81_07950"
     CDS             27542..28378
                     /locus_tag="PLV81_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10071.1"
                     /translation="MNENELNKIYEIILQRLHQPFIVDSSVVHFAESTLGIDPQELAE
                     HISDENGIIDLIIIPDNHFRKNIEPFIPTYGISSEDINTIICNIEKAVHNIYINIEGK
                     HINCSNPSYYRAFIHKLYLHKENIAILPQKELFNNYIAARIAIRLFAKNHDLYMLQKI
                     ADALVAESEESLVYDSIKLYTSIVDNETDVYTALSIHKQRLQKQLWDMYEFKRLMETY
                     SMEFLMSLRKTFPVVDPIKAQYQIKLIDAMSTALFGKPAKSFAPEIEIDNDNYKDLLN
                     TI"
     gene            complement(28381..28911)
                     /locus_tag="PLV81_07955"
     CDS             complement(28381..28911)
                     /locus_tag="PLV81_07955"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOM10072.1"
                     /translation="MKYYEAIEKFKTYNIVLLCGLYGSGKTEFANMYFKNSGLYRISR
                     LELRKLMYEMTHFGEPWAAEKFTEEDDVLAKHVERKITEHFIQNKRNVLIINTFMTKK
                     SRQRFINIAKENKKTIGAIFLDTPLEKCIEQSKRLSLTIPEYVIRQLYNKKELPTKLE
                     GFNDVLIINDFTLNQQ"
BASE COUNT         9353 a         4991 c         5688 g         8962 t
ORIGIN      
        1 gcgcaattca ctaataagcc tatttactgc tgcagataat atgcccacct catcaccttt
       61 aataatagcc agactttgtg ttaaattgcc ctgggcaacc ccacttaaca cctcacgtgc
      121 tactttcaat ggttctaaat ggcgtttaat atttctaaac agtatgatag ttgctatagc
      181 acaccagaat aacccaatac ccagcgacag tagcgctaaa agttttgaat attcattcat
      241 ctcagctttg ggaatgcttg caatcagtaa aaagcccaat ttgcttctgg tgcagtacgc
      301 aaatgaatcc tcgttattag aactatatga aataaataca ttctgattct ctaaaagttt
      361 tttgccccaa tcataccgca aaagagaatc atttgccata tcacgatgtg gatgcaccag
      421 acccgcacca tcattatagg taataaaaat atagcctgtt ttacctataa ccgcattttc
      481 cagaatgaat tttccaaatg aatcaaccgg gatgagagct gcatatattt tgccatttat
      541 gatatcaccc gttgcaaaat atgctctctt ttcttttccc agaaaaacag gttttgtaat
      601 aaaacttgtt gaaccctcaa aggttttcaa tgaacccaga gctttttcaa aaaccccgtc
      661 atatctgcct tttcctgaaa ggataacatt gccgttatcc agtattgcac aatctgtata
      721 tatttttcta agcgtaacaa tgcggtttat ggtatttcga tcaggaaatg aagcaattaa
      781 gctacaatca ttttttgtat attcaaaaaa actatctaac ttgtgacctg tttgctttgc
      841 aatagtatca atattgtttg caaacatctt ctgcatataa ctatgtgaca gcacaatgcc
      901 aaatgtaaca agcataatga caagcagaac attaacaata cttgccgaac cagtgatgat
      961 aggaataagc gaaccaaacg ctgttactgg ttttccgctg ataacatccc tgacgtcatc
     1021 aaatagcccc tcctgtgtta gcctgttaat agtttttggt ataaaatatg aaccaaatat
     1081 gattgatata gcaatagata aaattccacc aaaccacagg tataaaagct gatcaaagct
     1141 aagcccgtag aataaagcgc tggtaatact gaatatagca aggccaataa cccactgcgc
     1201 aataaggcca agcgaaaaca tgaccaccac actgcttaac cgctctttca atataagtgc
     1261 atgtttttcg gaaaaagatg ctccttttgc ttttgctgta aaaaaccagc gaaatggtgc
     1321 aaacaaaaat tgataccagt aatacgtcat aagtgttacc actataaaca caataaatcc
     1381 gggaacaata acaatatgta gattagaaaa gcttatttct gatttccaga taaaaaacaa
     1441 aaccgttagc ggtgcaccaa ttaaacctga aagagcattg ccatgaacaa gcatatcaag
     1501 ataaaaacgc cgatacagtt taatatagtc cataattgcc tcgcattttt ttgaaattac
     1561 tatataattt ttaaaaggta tcgagacacc tgcacgttat gagagagcag aaaaaaagta
     1621 aggaagctgt aaccagttta gcacttgaat gataaaattt taaaaaatta agatgagtgg
     1681 acgttttttt atttctgccg attacgattt tattgttttt tgaatgattg ctttattttt
     1741 agttcccatt ttttcaagca gtggccacac cattactacc agccgcggga acaccattat
     1801 aaaacgtgtt ataaatccct gcgatgacgg caccacaatt tcaggttttt ttgactctat
     1861 tgctctgacc acagcctttg ccacatcttc tggtttttgc ggcttgctta aaaacgtaag
     1921 cggcgaacca ccatgtgttg cttcatactg caacatgggc gtattcactg aatcaggaaa
     1981 taccgttgca accttaatat tgctcttttt taattctata tgcaatgtca aaaataaacc
     2041 acgcaatgca aactttgttg ctgtatatag tgaactgtag gtttcgggca ctattccagc
     2101 aagtgaggcg atagttacta ttgatgggct tttgcttttc ttcaaatatg gcaatacctc
     2161 atacgtacaa ttgattggcc ccattaaatt aacctgaatc tgacgcatta tttctttata
     2221 ggaagcattt tcaaaaaggt ttggttcaat tattcctgca ttattgacaa gtatatcgat
     2281 agctccatac ttttttactg ctttattaac cgttgacttt actgatttaa tttcagttat
     2341 atcacagtac aggccataaa ctttatcacc atactgtttt tgcaaatcag ccagcgcttt
     2401 tttattaata tctacaagca cgcacgttat attcttcttt ataaattcag cagttatagc
     2461 cctacctata ccgcctgccg caccagtgat aattgcaatt tttgtactca tagtattgta
     2521 cctctatttt aatatatgta gcgatgcaac gtattgcacc acaatatgtg tgttattttt
     2581 caattactga taaaaattca cccatttgtt tgcacatact attaaaatgc ggattatacg
     2641 tcaaccgtgt aataacttta tacttttcaa tattttcttc ggtattttcg cctttccata
     2701 tttcaggatc tgataaacac tgctgtcgtt caggactatt tggtttgtaa tataaacaat
     2761 tacggtaatc gccatttttg ccgtagtgcg gatcatcggg tggtatagtg atacatatat
     2821 gcggtatatt taaaattttc tcttgaggaa tataactgtt tacagtgata acaaaattat
     2881 cttctccatc ataatctttt tcaggctgtg tggtatatag aacaaacatc ttattacccg
     2941 ttacataatt tttcataaca aagagcgttc gttcagtact tactgtacga tcatctatgc
     3001 tttgagcaac aaacacagga acctgaattc ttttgccttc atcaaacaat tcatcaactt
     3061 tctttataag cctgtatgtc tgggttacac cgttaaatgt aaatgattga tacttggtat
     3121 aatcggtatc atcttctacg ctttcccaat ccttaaatgt accaacaata ggagctaccc
     3181 atgctaattt agaataaatg gcaaaagctg gtgcaaacaa aaagagccct tttacatcat
     3241 gattgttgtt agatagcaca taatgaacag acaacaatgc gccagttgag aatccaccca
     3301 tatacacatc ttttgcattc tttttaagct gtaacatgcc atagtgggta gcttttatcc
     3361 attcatcatt ttttactttt aataggtcgc ctggtacggt tccatgtcct ggtaaaagta
     3421 agccataaac aaggaaccct ttttcagtaa aatattttcc cacagcacgt aaaaaatacg
     3481 gtgaatccga caggccatgg ataagcaaaa tcccttttgg ataaatgcca ttaacaggtt
     3541 ttacctgtgg tttaaagata aatgggctat tcatagcaac aatagcatct ttattatttg
     3601 tttcaacacg agcatttgca ataacatgtg tcatcaattc aacatactgt tcatacggtg
     3661 tttccagtgg aatagtaaaa gcactgttaa aacctgattc ttttaaccgt gaaggattct
     3721 gtgcatagac aaaaactaat agagtaacta caaatgaaat acttatccat aactttcgtt
     3781 tcatcatttc cccctaatcg tagaatttca caaatgttat tccgaatctg attcggaatc
     3841 tattgctgta ttaatattct gaaccttttc ctgaaataaa attgggataa ggttcagaat
     3901 gactttgctc ataatatttt catagtatgg tttcttagcc cggtgaagta tgattgttta
     3961 ccgcagtcat aaatgccctt atattgtata atgatttata tccaatattg cttttgcaac
     4021 tactttttat attgattttg ttagaggttt taattactaa aactcactac caaaactgct
     4081 gatttcatct gtaaaccgtt taaaaatgtt ttgttgtacc atgatgtttc attgcgtaac
     4141 cagccaccaa gactaagcga tagtgatatc gatttactgc tgagccacgt aagtgccata
     4201 ccaaccatgg aagcttttat attgtttgct ggtacatcat agtttgcagt tattgggaaa
     4261 ggtacagtag tagtgatttt tgctgtagca tcaggtttat tcaggctgta gcttacaaga
     4321 taatatggtg ttattttaaa caagtcatta tatttgaagg aaggagctat accaaacgaa
     4381 acgccaaata aggtcgcaga gctgtcaact gatagtttag tgtttaaaat ttcaggcaat
     4441 gaccccgtac cgttatatct ctgtataaat atacccgcaa acagggggag tgaaaattca
     4501 ctggattcaa ccacatcata ccctatgcct gaatataacg agattataga ataatcaact
     4561 ttcatctgat aaatattagc atccaatccc ttcaagaaag cagatgataa tcttccatca
     4621 aaatgcatat atgcagcaat gccatatata agcattctat cgctggtagc atacccaaac
     4681 cctgttccag cgccataccc ttttacgtca tttccgttga tttcagggtc atcatatact
     4741 ttttgaacag tgaagtatgc aggaactatc gaaaccatat agttatcttc gttgtaatgc
     4801 gtcatgggca caaaactatc agcaactatg gatgcagcaa tattggttat tccatcgata
     4861 gtttttgcgg atgggttaag atcaatttgt gtttgtgcaa acaaagtggg tgagctcagg
     4921 agaagaaata caagtagcga aaagataatt ttaccagaat atcgagttag tagcatctag
     4981 actccccctg attaagtttt ttatttctat aaatagatat tagatataac gctatcaatg
     5041 atgtttgctt atgatgacga atggaacata ttcatgatat ctatactgta aagctgaagt
     5101 ataagatgtt gcagatcaaa tagagtatca atgaagtttc gtaaccttaa tagcgttaag
     5161 tatgaaattt actaaaagga tctgtcaatg aaaattataa aaaaatatca ttctttatta
     5221 ctgaatgtga aaatatgaat aaaatataca tacagtatac tccagatgcc ttcggtggcg
     5281 gaagtacttg ctttatgtaa tataaaattt ttttaaggat tattacctaa acaaatacac
     5341 tataactcca taatggaacc gccatagttc aatagaatgt tgtctgatat gaagcattgt
     5401 tcattggttt taaaatatca tgtaaccatg aatattggtt gactattata ttattttact
     5461 atctgtttgg ttattaaaat actgttattt tcttaatcat ccatgttgta tattattttt
     5521 taaggatgcc ttgatatgtc tatactccaa aaatttttac gaatctatga taacaaagat
     5581 tatttaaccc gacaacaggc cgaggcatta tttattttat attttattgt tgctcttggg
     5641 cttgctgcaa tgggagtatc tatgtttatt atctataaat cttaccatcc tacacatgta
     5701 ggcgtgctgt tgtgcgaaat agtcttatcg atttcatttt ttcttaatta caaaggaaag
     5761 aatattgcag cgggatattt gatgcttttt ccgcttaata ttgtgttatg gtatataata
     5821 tggataataa caggagttga tgatacaatc acagtaactg attcaatttt ttatgtattt
     5881 cccctgatag ctacagctac gctgataaac aatcgcactt caattatagt atttacaatt
     5941 ttcaatgttc tatcgcatgg ggcatataca gtctatttcc ataaaacagg tgtcttgaat
     6001 gcaaaacaat ttctggattt attacaggat gggattatag ccattagtgt gtttggaggc
     6061 atatgtgttt cttttataac aatgaaaatg aaatcacacg ctctaatact aaattctgca
     6121 aaggaagcac aggataataa atcaaaaatt gaggaaatgt tgcaacaaac atattcagtt
     6181 tcaaaagacc tggcagtttc aacctctcat ctggttggca tgactgacaa cttttcacag
     6241 gcaactcaat cgcaagcttc aacactggaa gaaattacct cttcggttga ggaagttact
     6301 gccagcggtg aaggggtgct ggcaatggtg caacatcagt caggtatggg ggattatgtt
     6361 aaaaacaaca tggattcttt atatcagata gtaaccgaag taggagaaaa aattaaggaa
     6421 gcaatagaaa taagaaatgt cctgaatgat ttggttggcc agtccagaaa tgaaatccag
     6481 atcacgctga atgatgtgca taatgcgctt tcacagttcc atgatatgaa gaatacagtt
     6541 gagataattg aagatatctc agataagatc aatttattat cattgaatgc agctattgaa
     6601 gctgcccgtg caggtgaatt tggcaggggc tttgctgttg ttgcagaaga aattggcaag
     6661 ttggctgata acactcaggc taatttgaaa ataataaacg aaatgtttaa caaaagtaac
     6721 atcggtatta cacaggcatc aaaaaggctt gatgaattca tccatctact caatgaaatg
     6781 ataggaaata ttgaaaaact atcgggcagt attgatgaag ttgtaaaatt agcccgggat
     6841 gaccttaatc tcaacaggca aacacgcaat tcactggagg aactattgca ggaggcgggt
     6901 aatattttaa gagccacaaa cgaacaaaaa caagctttag atgaaatagc aaaaagcatt
     6961 aatgtattta atgaaaccat tcaggatatt gcatcaagtg cacaaaatct ctctggattt
     7021 tctgttgatc tggctagtat ggttcaaaaa ttaaaaagta tgtcaggtat ataaacaatc
     7081 atttttccgg ttaggtctgt ttgaaactat tgttgattgg atcatatata ttttttaaca
     7141 acttcaccca tattaatgca atgagccgtc ttttgtaggc aaataacttt gggcaggcgc
     7201 ggctaactta tataaaaaca gcattgtaca ctatctcata tttaaaataa tgacccggta
     7261 ctataattgg cagataaaaa aatacttgac aaaatttatt ttataacgga tgttcgatat
     7321 aaataaatta aattttggag gttcgtaagt ttatgcaaca gcaagaatat gatgcaatta
     7381 ttgtaggtac tggtccaggt ggtgcaaccg ttgcccgtga gctatctaaa aaaaagaaaa
     7441 aggtgcttat gcttgagcga ggctctgacc ccataataaa gggcagttac gtgcaggcag
     7501 cttcaatgtt tatgcccggg aagggcactc tttttacgta cggtggtctt gccatggtgc
     7561 gtggcataac aatgggtggt agctcaatat tttactacgc cacggcgttt ccaccccctg
     7621 ttgccatgct taaaaaatat ggtattgata ttacaaaaga aattgaggag tgtaaaaaag
     7681 aactacccgt tgccccactt aaagatgaac ttattggccc catggcaaag cgcattatgc
     7741 aaagtgcccg tgagttaggc tatccgtggg ataagttgcc caagtttgtg tttcaggata
     7801 agtgcaggcc tgattgtgat aagtgtaatt tgggctgccc atatggtgca aaatttaatg
     7861 cacggatgtg ggcagaagaa gctgtcacaa atggtgcaac actgcttgta aatgcaaaag
     7921 tacaaagagt gctgtttgat ggcaagcgtg cagttggtgt tgagtataca cagggcttta
     7981 aaaaacacaa agtttatggc aatgatatca ttattgcagc aggaggtata ggttcaccag
     8041 ttatattgcg caatagcggt attaaaggag cagggtttga ttatttcttt gatccgctta
     8101 tctgtgtcat gggtactgta agggatgtta aaggtggcag ggaatttccc atggcaacag
     8161 ggcatcagtt tgtggaagat ggttatgtca tgaccgatat gactataccg cagttactgt
     8221 atcaggcatt tacggctgaa gttttgcgct ttgaccgatt gtttagccac agccacacat
     8281 tacaaattat gattaaggag aaggattcac tgggtggcta tcttactgat aaagaaggag
     8341 tgcgaaaaag actggtattt gatgacaaaa agaaactcat gcatggctat gagcgagcaa
     8401 aaaatatttt aaaaaatgca ggggcaaagc atatatataa atcatggtat gtggcagcac
     8461 atccaggagg tacggtaaag ataggtcacc ttcttgataa aaatttgcaa actgaaaagg
     8521 aacacctgta tgtatgtgac tgttcggtaa ttcctgaggc ctggggttta ccgccagcac
     8581 tgactttata cagtttgggc aaacggcttg caaagcattt agcaaaatca aagtagaaag
     8641 tttataaatt tttaccatag atatggggtt ggaaatgcca aaggtagtta atcacgatgc
     8701 ataccgtgat gagcttttgt caggatgttt ttccgtgttt gcacggtatg gttatgacag
     8761 ggttactatg cgcaagatag cgcaggtgct tggagtgtca acggggacac tgtatcatta
     8821 tttccccaac aagcaggcaa tcattaaagc gctttttgat tgggtcatgc gcaccaatgt
     8881 tggtgatgcg ttaaacagag tccgcgataa cgataacatg gatgagcgta ttgaaattac
     8941 acggcagttc tggaaagagt atggagaata ttatcaaaaa gttatgttgc ttgcatttga
     9001 cctgtggcgc aatagcagcg ttgatgaaaa tgagcacata ttttttgaat ttgcacatac
     9061 gtataaggaa gctatggtta aactatttga tatatcctat gaattttcag aaaagctgtt
     9121 tttaattttt ttaggttcag tgtttcattc aatcttaaca ccacatcatt attcatatgc
     9181 aaacacagtt gataagatag ttgaaatcat taaagctgaa aaaggtctgg acaaacaaac
     9241 aggtggtaaa aacaaaagaa caaacattaa gcttgtgcga tagttactgt gtaaaagctg
     9301 aatgattaca acgggaggtt ttgccaatga aaaaaaatct ttacctttct tccgtatgga
     9361 tgaagctggt tgccacacga gaaggaaaaa atttcctgca tcaatccatt aaagaatttt
     9421 ttacccagtg ggcaaacgat agcgtattaa aaaaagtcac tgcaatggta gtattgttct
     9481 gtatcaagcc ctttgttaga gcaacaccaa ccgataaaac catacaggaa atccttgatg
     9541 atgaaacctg gtggcaggaa ctaagtgaac gtttgctaca cacaattgct gagcatgata
     9601 tggctatggc aaagcactat gctgatgctg taagtaccca tgcacctgat gttacacgaa
     9661 aggtagctgg tgatatatgg atgtaccctg ccaaagtttt aacgcttttt gcctgtattc
     9721 cttctgttgc aaattgcaca atccgttgcg ccaacgaagc attacagcca atgaacaatc
     9781 aggcacccga tctattagct gatgttgtga attcacttat aaaagaaatt gattctgaat
     9841 cattaggttg tatggccaat caactgtgtg agcttatccg caagtttgat gcgggcgatg
     9901 agcttataaa agaatctgct tctacaccat ttgagcaatc tgtcaaaacc attgctaata
     9961 cggtgttgga aaaactggat gagaaaacaa agcaacagtt tttttatagt ttactatcgc
    10021 ttaaaaataa aattactggt ggactattag aaagccttaa agatcatcct gatatattga
    10081 gcatgctgat taatggtatt attctgagta aactgcataa tattcagact gcacgcacag
    10141 tattaagcaa ttaccgaata caggaaaaca tgactgattt ccccgttgaa gagattgcgc
    10201 atttttgcaa tgatctgctg cgtttggttg tttcagttca tgctaccaat aaaccgttgt
    10261 ttgattcact gataaaccgc tttgctcatg cgctggatac cagtgccttc caggatttta
    10321 ttactcaggc aggtggtgat tgttttactg cgctaaaacc tgtgcttggt tatgttttcc
    10381 cctttgtact tacctgctgg acagagctta tacaggaaga agatgaaacc atgcagcagg
    10441 cacgcaaagc atttgcacgt gcattattga aagatgtgga ggcagacaat gtttgaagaa
    10501 ttttatacag tagcccaaac aatcaataat gaatatattg ttaaggcaaa agagcagggg
    10561 gtacaatgca ttggctatac ctgttcatat tttcctgagg aaattttaca ttcactgggc
    10621 ataatcccat atcgcgtgaa gggacttaat gtatcgtcgc tttcggttgc tgatgctgcg
    10681 tttggtccat ttatatgcag ccacccaaag tgtttgttgc agcactttgc tgatggagat
    10741 tattcatttc ttgatggcat cgttgttaca ccaggctgtg attccatgcg aaggattgat
    10801 gagtgtattc gtaaaactgc tgtcaatctg aatttgccgg tggtcccacc atttttcttc
    10861 cactatgctg ttccacacaa aattactgaa tacagtgtaa agtggcttgt tgatgaatta
    10921 cagcggttta ttgacagtgt tacagaacat ttttcattgt cgttctccta tgaaaaattg
    10981 aaggagtcaa ttgcttttta taaccgtatg cgaaagcgtc tggaagaact taacgccttg
    11041 cgcatgaact atcctgcacg cattgcaggt gctgatgctg ttgcagtgta tgttgctggg
    11101 ttgtctatgc cccgtgacag gttttatagt atggtagagt cgttgttatc acaatataca
    11161 ggcaccaacc ttgaaggccg caggcggctt atgcttattg gaagtgccaa tgatgatata
    11221 gactttatca aagccattga aggcgaaact gcagtggtgg tggctgacac attatgttat
    11281 gggccccggc ttgatgttga ttatgtggat gagagtgatg agccattgca tgcactggca
    11341 ctgcggtacc ttaaccacag ccattgcccg cgcatgtatg gtggatataa acagcgcctt
    11401 gcaaaagtag tggaagccat agaaagagca caggtggaag gtgtagtgtt gcagaatatc
    11461 aggttttgcg atctgcacgg cgctgaaaat gggttgctag agcgtgacct ggagaaaatg
    11521 ggaataccct gtttgcgcct tgagcgcgaa tatggcccaa tggctgatta tgagcgcatg
    11581 cgaatgcgca tagatgcttt tatagaacgc ttagaagtaa ggagattggt atgcgacagg
    11641 ccctaaaaca atataacttt gactggatgt tatggcgtac gtatgaagct ggttctcgtc
    11701 tactggcagg aaccaagcag gaatatgata ttgcattacg ctatattcca tatttcaggg
    11761 accctgttaa agaagttttc aaagcaaagg cggggcaatt atttttacag atgactgcaa
    11821 catacctgga taatattgtt catgcgcatc agcgagggaa aaagacgtgc atgaccacat
    11881 tctgcttttc accggcaata gtgtatgcac tggatatggt tcctgtaacg ctggaattgc
    11941 tgacagtaat gatgacattt ttatataaac ggggtaccgg tgagttttta gattactgca
    12001 atgaagctgg ctttacggaa acatcgtgtt cttcgcagcg tggatcaatg ggtgcactgt
    12061 tagctgacat aggtacgcca atagacatgg ttgtcactga ttcaccgggt gtgtgcgata
    12121 ccaacgccaa tgcattttct ttttttgcag catataaaga tttaccattc tttcaactca
    12181 acatgccacc atggttggtg gaagagcgtg taaccgagta tcacgtgcag gattataagg
    12241 cactggttgc gtttcttgaa gagcagagtg gcaaaaaact tgatattgat agattgcgcc
    12301 agatacttac cgaactgcaa aagcaggatg aactcataaa cgagcttgag caattcctgt
    12361 gttaccggcc tttaccattg cctgttacta cctgcctttt tatctatgca ggtcgcttta
    12421 tgtttgctgg tatgcccatc tacacacgct tgcttgaatt gcttgtcaat gaaggaaggg
    12481 aaaacgttag gctgcatcaa cagggaattt acacttcaca tgaaaaatca cggggatttt
    12541 tttgctacat agaccattat acacatagct tgcggttgtg gcaaatgcta caggaactca
    12601 atataggcta ttcaggaaat attttgagcc atttctgggc tgatagcaat ccgccggtaa
    12661 tccagaacaa ctgggaagag gctgcgtatt ccattaaaac cggaacactt gaagatatgc
    12721 tgacaagtat tgcacagata aattcacgta tgccaatgat aaaatccatt cgtggaccat
    12781 atgattcgcc gtatatgtgg ttgcaggata cactggcgct ggcaagcatg tatagggctg
    12841 actttattgt ctataatgga acaccgggat gccgaaacac ctggggtatg gtaaagttac
    12901 tggcacagga taccgaaaag gcggggttcc ccacgcacat catgtatgca gatgcatttg
    12961 atgaccgcgt gcaatcgtgg gatgcaactc gtgaccgctt tgaggagttt ttgcgtgtac
    13021 ggaggctcat atgattgttg ctggctgtga cattggttcg cttacaacaa aggtggtttt
    13081 gtttgatggc aaaaagatac tggcttcatc aatcattaaa tcaaaaccaa aaccagaaga
    13141 cggcgcttat gaagcgctac agcaagcatg tgcgatagca actgttaatc aggatactat
    13201 tacatattgt gttggcactg gttatgggcg caaacgtgtt ccctttgcaa acgatactat
    13261 atcggagata tcgtgtcata caaaaggtgc attctatctg tatcccaatg tgcgcaccat
    13321 tattgatatt ggtgggcagg attgcaaagt tatttcactg aatacacagg gcgaggttga
    13381 tcgctttgtt accaatgaca aatgtgctgc aggaacaggc cgctttttag aagtgatggc
    13441 taaagtactg aaggttgaat tacaggaact atcgaacatg gctaaaaaag gaaatgaggc
    13501 tattacgttg gcatcagcat gtactgtgtg ggcacaggcc gatgttataa aatatcttaa
    13561 cgaagggata agtaaggaaa acattgcctt aggtgtatgc aagtccatgg caaaacgagt
    13621 gggagtactg gtaaaccagg caagcataaa gaaagatgtg tgcattaccg gtggtgttgc
    13681 aaaaaataca gcagtggtta aggaacttga aaatcttttg ggtgttacca ttataaaaat
    13741 aacaaatgtt gatccgcagc ttaccggagc attaggtgct gcgctatttg catatcaaaa
    13801 agccggaggt aaatgatgat agtagccgga tgtgatattg gctctaccac agcaaaggct
    13861 gttatacttg atgatagcac tataattgca acgatactgc ttccagtgga aggaagcccc
    13921 aaagatgcag cccttctgtt actttcttca gtacaggaaa agacaggcat tcagccttca
    13981 caaattgaag cctggtgcgg taccggctac gggcaaaaaa atattcctta tgccagcacc
    14041 gttgaatctg agattgtgtg ccatgcaaag ggcgttcaat ttattgatcc agatgtgcgt
    14101 accataattg atattggtgg gcaggatgct aaagtttgcc gcattgatgc atatgggaat
    14161 gtgcagcggt atgtatataa tgataaatgt gcttcgggta ctggaaggtt tttagaaatc
    14221 actgctgacg ttctggaagt acccattact gaacttgcca ctcttgatgc aaaaggagta
    14281 aaagaggtta cattgtccaa tcaatgtgtg gtgtttgctg aaacagaagt tatttccctg
    14341 ttaaacagtg gtgaagatat gggtaatatt gttaaaggat tgcacagggc actggcatcg
    14401 cgagtggcat cgcttgcatt aagtattgga gttgaggaaa aaatagcttt tactggtggt
    14461 gttgcgcaca atgctggtat ggttggtgca cttgaacgag tccttaaaac aaaattggta
    14521 gtacatgaat attgccagat gatgggagcg ttgggtgctg cattaattgc gcaggaaagg
    14581 attaaaaacc gtgttgcatc ctgatataaa aaatgtcctg gagtatgtgc aaaaccataa
    14641 aaaaggaaca atatattctg taaaaggcaa agaaccgtgg cccgaggttt tacatgctgc
    14701 aatgatattt aaagacgaca ttggatgcga gttgggtggt ccatttggtt cattagccct
    14761 gcattgtatg tatgatgaag tagttgatgg cagggtaata gtaattggca gtgatatgct
    14821 tcaaaaacaa tcagtgtggg gcaggttaac actgatagat gcagcagtag atgatgagga
    14881 aagttattat agctttttac agcaggtaac actgatacca gtacggcact caatggaagg
    14941 cgttatgata cgaatacagc cttctgagta taaagaatgg ataaggattc acaaagatta
    15001 tataaaaacg cataccgtaa ttcaattatt ttccaggtta tatacatggt ataaacagtt
    15061 gccgtgggta agggccgttg aatttgttgc aataacatca tgcagtgatg acattgcctt
    15121 gctgcttccg ctgattgaaa aacacaaaag tattataaag gcatttgaaa agcgcctgag
    15181 tgaagatata aaaaactgca atgcctgtga taacaacgcg gtgtgttcta caattaactc
    15241 gttatttgaa aaagggccat aaccatggat gaagcaaaaa caaaagtcat tgccttttgt
    15301 ggaaagggtg gagcaggcaa aacatcgctg tgtgcactta caatcaaata ccttgcccat
    15361 agcaaaaaac gagtgcttgc cattgatgct gatccagcgg gaggcctttc gtgggcgctt
    15421 ggattaaaac ctcacaaaac tcttgatgat cttaggttgt cgttacatga ggcagttggc
    15481 actaaggcag tgcagagtac tgatgaactg tatgcaatgg caaactatga actacttgca
    15541 agccttgtgg agcatgcggg ttttgatttt ttgagcgttg ggcggcctga ggctgaagga
    15601 tgtttttgca gtatcaatgc tttccttcgt cactgcattg gcaaacttac agagcattat
    15661 gagtatgtgt gtattgatgg tgaggcaggg cttgagcagg ttaaccgccg cgtacttgaa
    15721 acagtgacac atttaatcat tgttagcgat acatcaaaac gaagcataga aattgcacgc
    15781 aatctttttg aactggccag gcagcgtaca aaaactatgc actgcggtat tattataaat
    15841 aaaatgggcg atagttcctg ccagatagat cagcttccgg tatggggcat gttgcctgta
    15901 tctcgtgcca ttgcagtata tgacagtgaa ggaagaagtc ttttggagtt aagtgacaat
    15961 gaagagtatt acaccatgtt taaagcgata ctaaaaaaat atatgtgaag ccagctaatc
    16021 cttatttttc cttacacaaa cttcgcaccc ttaattgaaa gtagatacta tggccattaa
    16081 atcagggaaa aaatttttga tcatcagtgt gtatatttga tgaaaggttg tgttccaaat
    16141 agggaacgca gaatccataa caaaatgaaa aaaaagatat cattacaata tcataatttt
    16201 tacaaatatt aaaaactcat ggcaagataa aataatgatg catgatgcag agaacatgga
    16261 atacaagatt tttgtatttt tcttgaaata agaaatatca cgaagtatac atataaagaa
    16321 gtgcattata aggtattacc atgaacacac accattttga aaattctttt gatatccttg
    16381 attattttaa tgatgctgtt ttcatcatta cgtatgatgg gagcattgta tatgccaata
    16441 aaactgcgta tgaccgatta ggatattcaa aagaggaatt gttacaaaaa aacatcaggg
    16501 atattgattc gcctacctat gccaggttaa tccctgaacg tattgaacag tttaaacaac
    16561 gcggttcact ggtgtttgaa tctgagcaag ttactaaaga tggcagtatt atacctgttg
    16621 aagtaagtat tcattcaata ctgtataaca gtactccctg tataatcagt gtggtgcgag
    16681 atattacctc gcgaaaacag gctgaaaaag aattacgtga aagtgaagaa aaatggcgag
    16741 ctatcactga aaaccttaca gatattgtgt ggatagttaa tcttcaattt gaaacgatat
    16801 atataaataa agcggtggaa aaattatttg gatttacagt agatgagtac ctgcagcgta
    16861 aggttcaaga aaaatatcca cctgaagtat tgcaggatat atataacaaa cttatcgaag
    16921 aatttgaaaa tgaaaaaaaa ccggggattg ataaaaacag aaccaggatt gttgaaatac
    16981 aggaatataa aaaagatgga acacttgccg atttaagcgt tcatgttaaa tttttacgag
    17041 atgaagcggg caatccaaat gccataatag gtatatcacg ggatataaca gagcagaaaa
    17101 agaggcttaa ggaaattcaa cgctataaac tcatttttga acaatccatt aatgagatat
    17161 atatttttga tgctaaaacg cttatgttta cctatgtcaa tcaggcgtca attaacaatt
    17221 taggctatag ccttgaagag ttacaacgta tgacgcctgt tgatataaaa cctgaattta
    17281 ctctacagca atttaatgaa ttattgtcac cactcagaga aggtactgaa aaaagtatag
    17341 tttttgaaac cgtacataaa cgaaaagatg gcagtacgta tgctgtggaa gtacatttgc
    17401 aattagtgaa gatagatggc gaagaacatt ttgcagcaat aatccttgat atatccgaac
    17461 gaaaacgtat tgaaaaaagc cttaaagaaa gtgaagaaaa attccgtatc ctggcagaat
    17521 caacacccat gactataatg atgtatcagg ataacaagtg gatttatgca aatcctgcag
    17581 cagaagcact tttggaatat tcagaacagg agttgtgttc tatgaacttc tgggatgttg
    17641 tccatcctga tgataaagct attgtcattg agcgaggcag caaaaggcaa aaaggtgagg
    17701 atgcaacagt aggatatgaa ttcagagtaa ttagtaaaag tggcatgata aaatgggttt
    17761 tattgtatgg tgccacagta atgtataaag gtaagccagc aggattgata tcggtattgg
    17821 atataacgga aagaaaaaaa attgaagagg aaaaaaggaa attgcaggaa cagctcttgc
    17881 aatcacagaa gatggagtca attggtacgc tcgctggtgg tgtagcgcat gagtttaaca
    17941 atatgcttaa tatcatttcg gggcatgcag agctggcatt attaaatata cctcaagaaa
    18001 acgatgcacg taaagatatt gaagaaataa aaaaagcagc gcagaggtct gcagagataa
    18061 caaaaaaatt gctgacattt gcacgaaagc aagaagaaaa atcctctata gtaaatgtca
    18121 attcagctat tgattcaatg ataggaatgc ttaagaggct tattggtgaa aacatccagc
    18181 ttgtatggca gcctgcgaac agggagctat cggttcaaat tgatgaagtg agcattgatc
    18241 agatactggt taatctgagt gttaatgcac gtgatgctat tgacaaacag ggaaccatta
    18301 caataagcac acatagtgta tcaattaaag atgaagttgc attacagcac aatgttttac
    18361 caggagagta tgcattaata caggtgactg atgatggtat tggcatagat gaagagacac
    18421 agaaactcat ctttgaccct ttttttacca caaaagaagt tggcaagggg accgggctgg
    18481 gattatcaac ggtgtatggt atagtaaaaa acaatggagg atttattgaa gtgcaaagcg
    18541 aaaaagagaa aggtacaacg tttaaaatct atttacctgt attcatagaa agaagaaaat
    18601 ttgcacaata ccatgacaat gcagtaaagg acctatttca tggaactgaa acaattctta
    18661 ttgttgagga tgaagtttca attcttaaaa tgattaaaaa gatgttagag attttaggat
    18721 acaccgtatt ggaagcaggt gacccgtttg aaggattgga aattgtgaaa aattatattg
    18781 ggacaataca tcttcttatt actgatgttg ttatgcccaa aatgaatggt gttgacctgg
    18841 caaaacttgt ttcagcaata tatcctcaga ttaaaatatt atatacctca ggatattctg
    18901 ctgaagtgct gctgaaacat aatatcaagg ccagtgatat taattattta agtaagcctg
    18961 tttcatttgc cagtttggcg tctactgtga gaacgattct tgataattga aattatattt
    19021 cactaagcat tttcttgatt tcccgcttca atattttacc aacaccactg gtgggtaact
    19081 gatccataaa tacaataagc tttggcactt tgtatggggc aacatgttct tttaagtatt
    19141 gaagaatttt atgtttttaa ttcttcaggg tatggatcaa gatattgttg agatagtaag
    19201 tatgtatcat tgaatctatg tgcccaattc cttaatagtt ctataataac tatacggctt
    19261 attttatgtt cgcggtattg atcaagtaaa tgcagaaaat gttttttttc atttgaattg
    19321 agtttatcag agaaatatcc aaatatgtgt aatatagtat taacatgctg tttttgtgag
    19381 ggctttctgg tgaaagcagt ataaaatact gttttataat cattcaacgt ttttccaaat
    19441 gcaccttttt tatatccagc aactattctt cctaactggc taagttttcg ttgataatat
    19501 gtcatcataa tatatttatt ctcctggtga aattgtataa gtttttgaat agacacatta
    19561 tttgataaca tttctctgaa acgtgctaag gtgaagatcc tgattaagaa attttctctt
    19621 atatcttcat cacgcagcct gctttcatct tcaataggta aaaatccaaa tttattgatt
    19681 acttctgtag caaaaagccc cttgcccttg ctgtgaaaaa tgctacccat ggtgtcttta
    19741 taaatttttg ttcctgatac cccacacgat ggcgattttg ccttcaatat aaaaccatca
    19801 acaccctgaa gagtggaaag aaatgatgtt gcaaattgct gcatgagacc tgttacatcc
    19861 ttgtgtgtag ctggttgaaa aagcctgaat tgattattag cataatagac caggatatgg
    19921 tctcttggta cacccaaacc aattgaaact tccggacata catctaacgc tttgcagtat
    19981 ttcagaagca gcctgacaaa atcatcatta attgtttgtc cattatatct gatattttgg
    20041 ccattaaggc atgcactgac gcatataaca ggttttggaa attgcacaag attcctccat
    20101 tgaaaagtga ttttagttta catgatgtaa tatgtttgtc aataaaaaaa ttggttgacc
    20161 gtccatccag tttatgatat tttgtcacaa acactgttgg tgagaggata gtatgaaagt
    20221 taaaagtatt gccgatttat atgctttata taatccatta attcggcgaa gagctgtgct
    20281 gttatatata atgaacataa ttatactttg tttggttctg ccagttccat ttctggttct
    20341 tattattcgt ggtgattttt taaggccatt cattataggc attgctccaa ttataggatc
    20401 attaataagc attataacct tgcgtaaagg tatataccat ttctcagcaa atgctacatc
    20461 gcttgctact tcaattgcca ttttgttggg atcatatctg caatatcaag gtacacctgg
    20521 tataggttac tcttctatga tatatataat gccagcgggt attgtatttg catcactatt
    20581 ctgcagcagg ctgtggacta ccggtattgc aacattattt tttataagta atatatattt
    20641 ttattatctt aatacactgc agggaataat tgataaaaag attctattta caggtttaat
    20701 agatagtagc ctttcaatag ttttttgttt tgcattgtca tttctcattg taaaaataat
    20761 gaaagattca atgaatgaaa ttcaaattga atctgataaa aataaagaac agtatgcaca
    20821 gttaaaagaa cttataatag ctgttcaaca ggtagctgca aatttaaaag atgcttctgc
    20881 taaaatgcgt gatatgacag gcaggctatc cgaacaggct caaagccagg cagcatcggt
    20941 agaagaggtt acatcagcta ttgaagaagt caatgccagt atggatttgg taaatcagca
    21001 tgtgaaagat caaacagtaa accttgaact tttagattcc atgacaagtg attttactga
    21061 actgtttggc cagctacggc aaactatcga tacagtagcc cgtaaaatat atactacagc
    21121 agatcaatcg cgcaataatg aaaagaccct ggaagagctc aatataacaa tgcaggaagt
    21181 tggcgtaaaa tctcagcaga tgaattcagt agcagatgtt attgatacca tagcagaaca
    21241 gattagcctg ctggcactta atgcttcaat tgaggctgcg cgtgcgggtg aagcaggaag
    21301 aggatttgct gtggttgcgg atgagatatc aaaattatca gatcaaacag gagaaagctt
    21361 aaaggaaatt tacaatttga ctaaagaaac ggaaacacag atccaaaagg gaataaaaat
    21421 tgtccaggaa acagtgcagg caatgagtgc tgtaatccat aatgtacatg aaatatcaaa
    21481 cgatattcaa aacataagta aaaatatggc aatgcaaatt gaaaataata aaactataag
    21541 tgacaaaaca aaagaaacac atcagcatgc agatgaaata cattattctg cttccgaaca
    21601 gatgacagca cttggtgaag ttgcaaaatc tattgcaatg attaatgatt atactcagga
    21661 tctgtcaaac gctgtagtta atcttcttga aattgcaaaa attgttgata acgaggcact
    21721 tcagttgaaa gaaaggacac agatactata gcatgtgttt tgtgctattc gcaatcaata
    21781 agtacatcat agttagcata gtgttgcggg caaaattgtt tatgtttgaa tgtatatacc
    21841 gcatcaggta tacgtagtcg tgtaaccaga tatatccgtg gttttttaaa ttgaatatat
    21901 ccattataat atttagttgt aatatccagc gcatccatat cgcctgaaat agtaaaacaa
    21961 gatactgaat tatttttgta aaattcatac gtaaaagaat taaaacagta gcaaaaatcg
    22021 ccagcacaca gaacaacatt gttcccttta aaaaattcaa gccagccata cgtaggaata
    22081 ataaaatgtt taaaaccact ctgcatgtaa ttttcaattt ttttgatagt atcatccagt
    22141 tcatcttcag ggacaaatac aggcaattca ataaaggtat tgtttgcatc tgtagtttgc
    22201 aatttttcaa aatgttgcca ggtgtacgca gtgttacttg attgtatggc agtattattt
    22261 tttttaatta ttgcaatgtg tgcaataata tcgcttttcc tttgttggtt tatagattca
    22321 tgatgcttta tatacacatt atagagttct tcataaaatt tacgccgcag ctgttttaat
    22381 tgtgaaagcg gaataaaaat agtatcgttt atagttactg taacagttgc tataaaagga
    22441 aaatgtgctg tctgggaaaa aatagtttga atagtattgg gcgatagttc ctgtgtaatg
    22501 gctttctgga ttgggatagt atatgtatac tgtaccgtaa atgtatcaat gcaagcagaa
    22561 atgttaaact cagaatcaat tgtaatatga atctgcactg agtatggttt atagggaata
    22621 gcttttaatt tttttaaagt gttatctgat ttgctttctc ctattatata tacagaaagt
    22681 ggctgcttac ttttgattga aatataccac tccagagaat gtttgttttt gtcataggta
    22741 tagtctaaca gagtgccttc ataaactttt ttcccactgt tatccacaat tcgcaaacga
    22801 agcccttttt gcaatgggtg tctggtggaa attatacatg atttattttt tacctgtgtt
    22861 acagtaccaa tatatgttcc tgctacacct aatgttgtag cgtcaaccag tgcatcatat
    22921 gaactatcga acatatatcc tttggttgta ctacgcaaac ctggtgcttc ttttttaaat
    22981 ggcttgcctt ccatcaatga gtcaattaat ttcctgtaat atgaaaccac ctctttaatg
    23041 taccaggtac tttttaaacg tccttcaatt ttaaactttg taatgcctgc gttgatataa
    23101 tcagcaaggg atggaccaag tgcaagatct ttcattgaaa aaggatagcg tgttgtgcca
    23161 tctattatga acggatatcg gcacggttgc agacaacgac ctctgtttcc cgactggcta
    23221 aacaaatgcg aagaaaaaag gcagcatccc gaaaatgaaa aacacattgc tccgtgaatg
    23281 aacacttcat attcacaggg aactttttgt tgaatggctt taatctcatg tagcgatagt
    23341 tcccggggca gtataatccg ttccacaccc aaatttttta gaaaccgtgc atgcatgctg
    23401 ttgtggcaaa atgtttgtgt tgaagcatga atggtgaggg aaggataata tgtgcgtata
    23461 agctgtatta aacctatatc ctgaacaata atagcatcaa cggcatattc cattgcaaga
    23521 ttaagaattt cagccacctg ttccagttca tgatcataaa gcagcgtgtt aagcgttaga
    23581 tatatctttt tattatggtg gtgaacgtag tttaccgcat atttgaattc atcatgcgta
    23641 aaattttttg cgcgcttacg agcattgaaa tcctttaggc ctaaatacac cgcatcggct
    23701 cccgaatcaa gcgctgatac taatgcttca ggggaacccg caggtgcgag tagttcaatt
    23761 ttagtattgt tttttgtcat gtaaaaaata ttaatctagt atggaatagt taaaaatcaa
    23821 atagtttaaa accatataat ataataaata tatccaggta actgagatat atcaagaaac
    23881 tgctatatgt ataaaaataa gcgttaattt attgcagaca gtgaattgaa tagttcagga
    23941 ggaggtcccc agtagaattt taagcaatga atgccatttt cacggtaaaa atcaaaggtg
    24001 tatgacctgt tttcatagta acttggcaaa ttctcaggat agttatcgga atttatcttt
    24061 gccaatcgta ccttgaaata ttcaaaaaat tcaaccagca cattatctgc acaaaattcg
    24121 ataatatgtg caagcgacat cttccacagc ttcttcacat ccaggaaaaa ctcatactca
    24181 gactggtaca atactaaatg tacccgtttc caaggattat cagtattacg tttgcggtaa
    24241 gctatgcgtt taaatgcttt gtatttcctt ttttcttttt tagcagcata catgatcata
    24301 taaacaataa aagtacgaag agaaatgcct aatttttgcg catattcttc aaggattgca
    24361 atatgctgaa gagaaataca ggtagtagtt tcaaaataca tcgtttcctc ccaaagaata
    24421 aagatagttt atttaaaaaa atatataaaa tatacgttgt caaatcatat ttttcttaac
    24481 catatttaaa caaatgtaag gctaatgttg ttgcaatatt tttcacaata cagagtgata
    24541 ttaggatagg aacttattaa ctagtagact aaatgctaag tagcaaaaag aattaacatt
    24601 agtaaatgtc tataatatgt gaataaaaag aagaaagtta tattttaaaa cgtttatgtt
    24661 accaccaata gattattaac ttgacatgtt taatgaaaat ataacatatt tgctttatga
    24721 aaaatgcaca atcatttcgt gaattaaaag aatctgaaaa agaaaatagg aaaaaaataa
    24781 ttattgatgc tgcagaacgc gtatttgcaa caaagccttt taatcaggta acaatgcgtg
    24841 atatagctaa agaggcaggt ataactgcag gtgccattta tcgctatttc ccggaccagc
    24901 aatcattatt tgtggaagct ttcctgcggg gtgcagaaaa aataattaat gatcttgaac
    24961 aggttatttc ccgcagcaaa aatccattaa aagatactat cgccacattt ttaaaatttt
    25021 tgatgaagca tgatcagtat ttcaggatga tgacccattt tatgctggat gcacatttaa
    25081 accaggatct tgttgaaaag ctcaatgaag cagaacgaag gcttttagat caatttacaa
    25141 agttgtttga acaaaggtca catgccaggt tacttgctca cagtttattt gctgcaatga
    25201 atggcgttgt gattacgttt cataattatc ctggcaggag caaaaaagat atacaccagc
    25261 atatgaacag gttgggcgat atgctatacc agttattttc gtaatgatat agccatctgt
    25321 gcatcttcaa cttcgtgaag agcacagttt tgaaattcat gcaaaagatt atgacatgca
    25381 tgttcccaca taatacaaag atgatcgttt tcctgcccag gataaataaa attactctgc
    25441 aaacattccg caaatgatgc agcaatctca ttaattgatt tactgtttgc attgtaaata
    25501 aggtcgtgaa tgaaatcatg caccgggtac catgacgaag cacgctgaat gtcgcgatat
    25561 atttgtgtta aaagcacatt acgcggacct tcccataatt catttatcat catgtcacgg
    25621 aaaagccgtg gcaaacttga aaaatcttcc ataacgccat gcccgccaaa tactgagatt
    25681 gcttttcgta aaatatcagt tgcatcccat gaagcaacta tcttctgcag catgataagg
    25741 atgcgcaccc ggaaacgctt tctttgaatt tctacatcct catcggttac aaggccgcct
    25801 tttaatccac cgggcagtga ctgaaacatt tcatagattt taaatgcacc agcaagtgtt
    25861 cgttgtgcat agtgattaat ttcatcaagc tgtcgtttaa gtagtggaaa ttggttgatg
    25921 gggaatccaa atgcatttcg gaactgtgca tactgtgttg cttcgcgtga agcacgaatc
    25981 attgctgctg ctgccgataa ccctacggtc agccgcgagt gtgttaaaac tatgcctacg
    26041 acattggcaa tgcctcgttc tattggtccg gctttatagg cgatagctcc attgaatgta
    26101 atctctgctg tgggaagttc tgctgttcct aacttccatt taagcctgtc aatagtataa
    26161 ccattacgtt tttcgtttgt tttatcactc catgatggca tgacaaaaag gccaacatgc
    26221 tctgaccctt taggacgggc agtaataaca acataatctg catgtgttgc agaacagaag
    26281 aattttttgc cgtataaaag ccaatgatca ccattatcta ctgcttcaag atcattggct
    26341 gcaacatctg atcctccctg aatttctgag aggtactgcg caccaattgc aaactcaccg
    26401 tcaatgcctt ctttgcaatg ctgtaagatt ttttccagtt tgggatgatc atggtatttt
    26461 tccagaagtt caaccagtcc ttcggtacat actaaaggac acgcgatacc tgcttcgcca
    26521 ttttcataga taaggattaa ttttaccagg cgttcccacg gcaacgtttt tgctgaaaat
    26581 aatccctggc caaatacttc cttttccatt tcttcaagtt cttttgggcg tacaatgcga
    26641 tctatcctgt gattatgccc atcgtaatgt tgcatgaaaa ttctgttgtg tggttgtgct
    26701 attgtatcag ctatatcccg ccatctataa gaagccaatt ttgaaagctt acgggcagcc
    26761 tggtcaactt tttcaaaatc atcttttgca aaatagtaag ttaccttttg taaaaagggg
    26821 tcatctgcat aataatccac gtttttacgc cattctagat attcatcaaa attgtaacta
    26881 gtattcagtg ccataatgcc tcctcttaga aaatagtaga taatcttact gaaatttctt
    26941 gtgtcgattt gttgggatga gaaatatttt catttccata tcattctgat gaaaccagtt
    27001 acctgagaat gttgtggttt gaaacattag attaccatcc ttatacctaa gaatgacggt
    27061 taatgcttta ttctcatctc taaaagttac tacattagta attaagtaat taataaaaac
    27121 taaagtaaac aatgtttaca aaagtaaata taatttacta taatatattt tgtcaataat
    27181 ttcaatacgt attaataaaa attcttattt tagttataaa cagtatagct atannnnnnn
    27241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntggttat tgtgttacat tatcatgtgt
    27361 tcatgttata ttttttatgg catgttctct aatacagtta agtgattatt taaaataaaa
    27421 acgattgata atttaatact gtagtaccaa attgatattt cataatgcat gttgaaatta
    27481 ttgagttcac tttaatttgg catgtatctt taaagatata cttttagcag atgtgtaata
    27541 tatgaatgaa aatgaattaa acaaaatata tgaaataata ctgcagcggt tacaccagcc
    27601 ttttattgtg gacagttcag tagtgcattt tgccgaatca acattgggga ttgatccaca
    27661 ggaacttgct gaacatatat cagatgaaaa tgggattatt gatcttatta taatacctga
    27721 taatcatttc agaaaaaata ttgagccatt tattccaacg tatggtataa gcagtgaaga
    27781 tataaataca ataatttgca acattgaaaa agctgttcat aacatttata ttaatataga
    27841 ggggaaacat atcaattgta gtaatccatc atattaccga gcatttatcc ataagctcta
    27901 tttacataag gaaaatattg ctattttgcc ccaaaaagaa ttatttaata actatattgc
    27961 agcacgtata gctatacgtt tatttgcaaa aaatcatgat ttatacatgc tacaaaaaat
    28021 agcagatgcc ctggttgcag aaagtgaaga atcattggta tatgattcaa taaagctata
    28081 tacaagcatt gtggataatg aaacagatgt atatacagca ttgtcaatac ataagcagcg
    28141 tttacagaag cagttgtggg atatgtatga atttaaaaga ctaatggaaa cctatagcat
    28201 ggaattcttg atgtcattgc ggaaaacatt tcctgtagtt gatcctatca aagcacagta
    28261 tcaaataaag cttattgatg ccatgagtac tgccctgttt ggcaagccag caaaaagttt
    28321 tgctccggaa attgaaattg ataatgataa ttacaaagat ttgctgaata ccatctagta
    28381 ttattgctgg ttcaaagtga aatcattaat aatgagtacg tcattaaaac cttcaagctt
    28441 agttggtaat tcttttttat tatacaattg tctgatgaca tattcaggta tggtaagact
    28501 taaccgtttg ctctgctcaa tacatttttc aagtggtgta tccagaaaga ttgcacctat
    28561 tgttttttta ttttcctttg caatattgat aaaccgttgc cgggattttt ttgtcataaa
    28621 tgtattaatt atcaatacat tacgcttgtt ctgaataaag tgttcggtta tctttctttc
    28681 aacatgtttg gccagcacat catcttcttc agtaaatttt tctgctgccc atggctcacc
    28741 aaaatgagtc atttcataca taagttttct aagctccagt cgtgatatac gatacaaccc
    28801 tgaattttta aaatacatat tagcaaattc tgttttccct gaaccataaa ggccacacag
    28861 aagaacgata ttgtatgttt taaatttttc tatggcttca tagtatttca tggagtttcc
    28921 ctccaactgt aaaatttatt cctgatatca tttgtaatct tccagcctgt cagggcaaaa
    28981 aacttacaat gtcatttaga attcccaatt tcatcaggat aagaaatctt attagttcta
    29041 gaagtcattc tgagtgaacg gaatctatta aaagatgtct ttgtttcatt atag
//
LOCUS       DAORYL010000035        28126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1449_length_28126_cov_12.801290, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000035 DAORYL010000000
VERSION     DAORYL010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 28126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28126
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1449_length_28126_cov_12.801290"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1916)
                     /locus_tag="PLV81_07960"
     CDS             complement(<1..1916)
                     /locus_tag="PLV81_07960"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOM10073.1"
                     /translation="MYLDSLEIFGFKSFADKTKINFEKGITAIVGPNGCGKSNIVDAI
                     KWVLGEKQAKNIRGDKMEDIIFAGTDQRKPLSLAEVSLVINNTNRILNIDSDTVTVTR
                     RVFRDGESEYMINKSPVRLKDIEELFLDTGLGKASYSVMEQGKIDLILSTKAEDRRYI
                     FEEAAGISRFKVQKKESLKKLEDTTVNLERLNDIIKEIEREKDFKSKQAEKTKIYFSL
                     REKYKKNDIALQILRYRDFQKKLQKVSEDRQRIFDEREELSKKVALISSENENDEKRK
                     NDIQLQLFEWDKKLHTYTVKVEDIDGKTTRNKQLIQQQQQRIATTQLRIQEIEEAIKK
                     LTEEKEKNIEQSHTIIKNIASDKEQLENLYAHRKKKIQNIHNYRDTIEQKKHAIKDLE
                     TEVKKLRESLEIVIRQLVDAIEKRKHELESSEAERELVRERIHAALTGAEENLQKALH
                     NLSLNLVHEAIEVLQKIDFIALKHDIQLFENYEDGFRSILFDKTGIHAQKEQLDSSIH
                     KASADIEQLRNDISQLEISIRNEQEELEEVNNMITRVEKDLSKNENEKAWIDKHIQTI
                     ERQITEDTKQKTHLENEIQKIQQQIDDLTKEIEQWQNSLVEFNEKSNELKQQIHELVA
                     KRDEIEKRIIGRKQS"
     gene            2398..2607
                     /locus_tag="PLV81_07965"
     CDS             2398..2607
                     /locus_tag="PLV81_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10074.1"
                     /translation="MKVCYPIILLISIIFIAGSTYAKEVHYTLDDRDRIIRMEVKINE
                     MDKRFEQYSLYPCVAFSLIALFIVA"
     gene            complement(3085..3315)
                     /locus_tag="PLV81_07970"
     CDS             complement(3085..3315)
                     /locus_tag="PLV81_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10075.1"
                     /translation="MKTKYLIATMIAVLIFSVAIVISAEQKKKPTSAREICHRKCQEE
                     MDKCVEEKGINRIKCAKQYDECIDSCNALLNE"
     gene            3661..>3954
                     /locus_tag="PLV81_07975"
     CDS             3661..>3954
                     /locus_tag="PLV81_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10076.1"
                     /translation="MIQKLFIALISIIVVSSITACDRLNMYDIASRKSRTAYALIFNG
                     TTYRLAIAHPEYDKIYTSNFVFSAGSEGVSANNLTIIVYDSTNTYISNNDLTSW"
     assembly_gap    3955..4054
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4055..4511
                     /locus_tag="PLV81_07980"
     CDS             <4055..4511
                     /locus_tag="PLV81_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10077.1"
                     /translation="ELMQSFQSTPPTSGTYLGGFRTKNYFYVWYNGGNDSIFRTNGSV
                     DINYNDTTAWSNVVDVAITDDDKMYAIIFVSGTYNLLHIKGPMDFTTVLFLGTTGTFK
                     IDCLDSKHIIVAAYGNTDGYNGLFIYNVESKKIEKFITAEDAIALYVHR"
     gene            4755..5270
                     /locus_tag="PLV81_07985"
     CDS             4755..5270
                     /locus_tag="PLV81_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10078.1"
                     /translation="MIQLRSFVILSGLLISLPFISCSTIQLAQQKTQLYSTTSDTLTL
                     PKSKERIDKTPTYELFFELLDNTRCLDISVIAQDRNGKRFRCAFILEALVDTSLYPVK
                     PLKPIYTEFARNFDINWRFQSNIFICSDENDPLKRLTKGNYRLKISAFSNNYYTFTID
                     IKSSVPVEFRK"
     gene            5302..5703
                     /locus_tag="PLV81_07990"
     CDS             5302..5703
                     /locus_tag="PLV81_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10079.1"
                     /translation="MPKHYDDIESEDLYEMALQWASQGFYDKAIELVKQSIELNPNFI
                     GAYIDLAHYYAKKHRYNDAFHVLKKASKIDMCFHRLNYLMAKYAYKNGDYIAASKYID
                     RAIELSAEKLYIKAKAVIGKKSVKKLTKKKQ"
     gene            5895..6416
                     /locus_tag="PLV81_07995"
     CDS             5895..6416
                     /locus_tag="PLV81_07995"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOM10080.1"
                     /translation="MKKILLVLLVFFTASSVFAAGIGIKGGYSKMYDDYADAFDDTWN
                     IGIVFDLGTFLFNNLQFRPGIDYLSLENEDANFEYADVYGIHLDWYWFFMGKKQFSPF
                     LGFGPALNYYSFDDDRTNNDDSDAGIEGFAGVDFDLSGPWKLMLELRYLWHDIADRDT
                     TIVKLNVGLLYYF"
     gene            complement(6459..7019)
                     /gene="pyrE"
                     /locus_tag="PLV81_08000"
     CDS             complement(6459..7019)
                     /gene="pyrE"
                     /locus_tag="PLV81_08000"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOM10081.1"
                     /translation="MNDNQRLFEILYQKSFMYRDNPPFTLVSGKQSPYYFDCKATTLD
                     PEGCILVGRCMFELIKDLPADAIGGLTLGADPISLSTAMHAFAQGKVLYPLIVRKEPK
                     KHGTQKWIEGNTANVKNVIAIDDVITTGGSTITAIERLTESGLSVIAAAVIIDREEGG
                     REAIEEKGITVLSLFKRSDFDKKRLE"
     gene            complement(7016..8317)
                     /locus_tag="PLV81_08005"
     CDS             complement(7016..8317)
                     /locus_tag="PLV81_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOM10082.1"
                     /translation="MNLFDKNTYIPLAERMRPKTIEEFVGQKHLLSKGKLLYSIFEQK
                     KALSLILWGDPGTGKTTLAHLIASACNMDSYFISAISAGVADVRKVIDKGKANRTAGV
                     QTLLFIDEIHRFNKAQQDAVLGAVENGDIVLIGATTENPSFSIIAPLLSRTRVLRLYP
                     LQEDDLRAIVTAAIANDQELSHKNITLDEAALDALVQLAMGDARRALNILEAAAFLKG
                     QGTIDKDTVFEAYKEQSIAYDRAGDKHYDTISAFIKSLRGSDPNAAVYYLARMLVAGE
                     DPEFIARRMVIFASEDVGNAYPLALTVATSGMLAVKNVGLPECEIILAHMATFLASCP
                     KSNASYMALVNAKEIASSDVHEIPLYLRNAPTQLMKQLGYAKGYKYPHDYPGHFVDEE
                     YLPKEIKDLIFYAPSEEGNEKNIKERLTRLWPKKYKKEKGE"
     gene            complement(8328..11711)
                     /locus_tag="PLV81_08010"
     CDS             complement(8328..11711)
                     /locus_tag="PLV81_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOM10083.1"
                     /translation="MTHPEFMQKYKLKNHVRIVTAASLFDGHDASINIFRRIMIGTGA
                     EVIHLGHNRSVWEIVKSALEEDVQAIAVTSYQGGHVEFLQYMYDLLKEHNAASIKIFA
                     GGGGTILPHEIKELEAYGICKIYSPDDGRAMGLQGMINDLLSKSDYPVGNINNFDIEG
                     IKKKNIRAIATAISACENFKELVKDDIATVQQLTADDSTLVVGITGTGGSGKSSLIDE
                     LVLRFQQSNPDKTIAILSVDPTRQKTGGALLGDRIRMNAIYHPNVYMRSLATRGSKTS
                     VSNTISDALHIMKLAGYDLILLETSGIGQAQTDVLEYSDISMYVMTPEYGAATQLEKI
                     DMLDYADIVVINKFDKLGALDALRDVKKQVQRNKEAFDISPDEMPVFGTIASQFNDDG
                     VNVLFNHLIHTINAKSKTQFAVYTADKQKKDILYIVPPERSRYLSEIAETIRKYNNKV
                     QEESLKAQYCFSLKNSIEYYEQHGGDAKILKDLHQKYNEAYSSLDEESKKILQQWEDK
                     KKNYQGEYYTYKVRGREIKVKAFTQSLSHTKIPKVCMPKFKNWGDIIEWSMQENVPGE
                     FPYTAGVYPFKREDENPTRMFAGEGIAERTNKRFHYLSYTMPAVRLSTAFDSVTLYGC
                     NPDKRPDIYGKIGNSGVSISTVDDVKRLYSGFKLTDPNTSVSMTINGPAPIMAAYFFN
                     AAIDQECELYIQQNGLTDEVKRKIQELYQKRNATPPVYRAAQLPDGHNGLGLLLLGIP
                     GDMVVPAEVYHNIKNHTLRTVRGTVQADILKEEQAQNTCIFSVDFALKLMGDMQEYFV
                     KHKINNFYSVSISGYHIAEAGANPITQLAFTLANGFTYVEYYLARGLHIDDFAPNLSF
                     FFSNGLDPEYSVIGRVARRIWAKAMKYKYGANERSQLLKYHIQTSGRSLHSQEIEFND
                     IRTTLQALYAIYDNCNSLHTNSYDEAITTPTEESVRRALAIQMIINHEYGLAKNQNPL
                     QGSFIIEELTNLVEEAVIAEFDNISARGGVLGAMETMYQRNKIQEESLYYENKKMSGE
                     LPIIGVNTFLSKNGSPIQIPDEVVRATPEEKDTQLASLEAFHAVWKQESTVALEKLKT
                     AVKQHENVFETLMDVAKYCSLGQLTGALYSVGGMYRRSM"
     gene            complement(11991..12323)
                     /locus_tag="PLV81_08015"
     CDS             complement(11991..12323)
                     /locus_tag="PLV81_08015"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOM10084.1"
                     /translation="MMTKEEILKAAKSIIVEELQKFGYTVLEVYLFGSRAKDREKPDS
                     DWDFLIIVDDEIDFPRKKEITMLIRRRLVKLDISSDLIIQSYNIKKTRENNIAYLTYY
                     AIREGIPL"
     gene            complement(12495..12761)
                     /gene="rpsU"
                     /locus_tag="PLV81_08020"
     CDS             complement(12495..12761)
                     /gene="rpsU"
                     /locus_tag="PLV81_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOM10085.1"
                     /translation="MPVPLEVEVTDGDLERAFKNLKKRMAFEGIFKELKRRRYYEKPS
                     EEKKRKKEEAERRRLKKIRRFETQTKQRRFVAKPSGRGGSSQDE"
     gene            13121..13603
                     /locus_tag="PLV81_08025"
     CDS             13121..13603
                     /locus_tag="PLV81_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10086.1"
                     /translation="MSKKIIYTILSLLITICLHCSKQVLQSPPPEQSQIPDIQSTNII
                     LECLQKSNNGYPFVELSLIIENTGRYFVATVYGQIYQIYSKPPVFAPPYATCGVSIKN
                     NNTIHYFFVYANATTDIIAVQSLVEKVSDNYINTMDYQTIIKIPVQLHNRIQSTVTVK
                     "
     gene            13700..14647
                     /locus_tag="PLV81_08030"
     CDS             13700..14647
                     /locus_tag="PLV81_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOM10087.1"
                     /translation="MVIGLDIGGTNIKGVIVDSTGVIRAQTSVPTGKAILETEQYIIQ
                     CITILQELSHTNNNEIEAIGIGSAGAIDKHKGIVITSPNIPWWKNYPVVQQIQKLSGC
                     KTFLENDANCAVFGELWLGNGKHFKNWIMLTLGTGIGGGAVIDGVPYTGRAGSAMEFG
                     HTTIDYNGLLCKCGRKGCLECYGSATAVVNFTKSHISMYPDSTITKRMTQESLTAQLV
                     FEEAQKGDALALYIFNLIGQYLGIGIANLVNIFNPEAVILGGGLSGAHEFFMPHIIQG
                     VQTLALPGMKEDIHYCVTSYGNYTAAIGAAKYALDNLLE"
     gene            14671..15570
                     /locus_tag="PLV81_08035"
     CDS             14671..15570
                     /locus_tag="PLV81_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOM10088.1"
                     /translation="MFFTNQLLFSEQTLNQIHLYAGKWLDFIMVAFTQLGNEMFYILV
                     IPFLFWCVNKRIATIVGIAFLLSAAINDIAKFLFINPRPDAANLAPQIAELNRMYCPV
                     NSPGFPSGHAQSAVVFWGTLAYVVKHRLFTIFSLFLIIAISYSRLYLGVHFLGDVAGG
                     IILGTVIFLCVIALIPHYDRLWNTGIIPAVVFFVILPFVIAIVLTGNEIAKSLGVLSG
                     FAIGFILNNDKSEEITFSIVPALVKFVIGITIILGIKEGLKIVFPSSNVFDFIRYWFM
                     GLWVSYGAPALFMKIPYLSKKSE"
     gene            15778..16815
                     /gene="pheS"
                     /locus_tag="PLV81_08040"
     CDS             15778..16815
                     /gene="pheS"
                     /locus_tag="PLV81_08040"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOM10089.1"
                     /translation="MYMKKQLQDLYKQTISAIDKATTIDELEQLRVKVLGRKGDLTQI
                     LRSLGTLPQEERVEIGKLSNELKEKIESALENRKTVLTEQQINKIIMEDWLDVTLTMP
                     GTLKHYDRGHLHPISQIQYEIEDIFTSMGFEILDGPEVETDYYNFEALNIPPYHPARD
                     MQDTFWTEDGNLLRTHTSAIQIRGMERLKPPFRVIGPGRVFRYESIDASHENTFYQVE
                     GMMVDKDISVAHLIYFMKTLLKEIFQKETTIRLRPGYFPFVEPGFELDIHCLICGGKG
                     CSVCKYSGWVELLPCGLVHPNVLRYGNIDPDKWSGFAFGLGLNRLVMMRYGINDIRHF
                     LSGDIRFLYQF"
     gene            16837..19233
                     /gene="pheT"
                     /locus_tag="PLV81_08045"
     CDS             16837..19233
                     /gene="pheT"
                     /locus_tag="PLV81_08045"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOM10090.1"
                     /translation="MWISLNIIQDMVDIKDIPVADIANRLTMSTAEIDSIEYSHELLK
                     NIYTAKILEVAKHPNADKLTVCKVDTGKGVLQVVCGAPNHKAGDIVALAPVGTKFTET
                     FSVTKAKLRGVESEGMLCSERELGISDDHSGIMVFPQNTPIGVTLAQLYPNLIDVRLD
                     IDNKSITHRPDLWSHTGFAREIAALFNKPFKYPVNYDLAKDFKSKETLKVTIKNPEGA
                     PRYCGLMVNNITIKESPDWLKARVEAIGMRPINNIVDITNYVMAELGEPMHAFDRSKL
                     RGNEIIVRFAENGEHLTTLDGQEHVLTSEDVVIADSGGAIALAGVMGGGNSEIEDNTT
                     SIVLEAANFNPVYIRRTANRYNLRTEAAIRFEKSLDPELCPAAIIRCYELIKMLCPEA
                     EAVTPIIDAYPVPAKPVSISTTTDFIRRKLGHSIDDNTIVSILTSLDFKVTKNNNNLI
                     IDVPSYRATKDISIPDDIVEEIGRIYGYDNIPEQPPFVPCAPPSRNIKRTFEREIKSI
                     LVNDFHMTEVSNYSFVGEALLNKLQLNENKELQLKNPLSKEQDRLRRSLIPNVIVNIT
                     TNQRFFDDFRIFELGRIYLKEDRLSKDLAKENTRVTGVIYSKKTAYPVFYDAKACCIG
                     VFDKTFRNNYILKPKSDNLPPYAHPVRSLELLIHNKPVALVCELHPKIANEFEIKGQV
                     ALFDIDVDLLLSLPQQDIVFNELPKFPDVPFDISVLTDEKEYAATLVDMLYKADKEHI
                     ADIEVISVYNQPPIPQGKKSVSLRITFRAKDHTLSPEEIDNLQKKVIAFVEKHGYKLR
                     "
     gene            complement(19488..19901)
                     /locus_tag="PLV81_08050"
     CDS             complement(19488..19901)
                     /locus_tag="PLV81_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10091.1"
                     /translation="MAKSKHLYRWIKAAKIMFGKHDIEVIGLIIDKKNKIMKVIYESP
                     ENYNPNNFSVKHKTNEITQNIILPLRFKVVSWNHLFQHVDSNYELQKGEVELFIKPKY
                     FTQDLWDEFTMFKKEETALKQNRAHYPWEREFINE"
     gene            complement(19823..20194)
                     /locus_tag="PLV81_08055"
     CDS             complement(19823..20194)
                     /locus_tag="PLV81_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10092.1"
                     /translation="MKKIIISVIVILLVILIGFVTYVANKTVRVNETDIPGFTPIKND
                     ILADKYCPYIISNSEYEFPYAVYYRASVDDKGNTYIAYHYFWEREVNNTKGFVPWLSR
                     NIYTGGLKLQKLCLVNMILKL"
     gene            complement(20224..22929)
                     /locus_tag="PLV81_08060"
     CDS             complement(20224..22929)
                     /locus_tag="PLV81_08060"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOM10093.1"
                     /translation="MNKKFLIALIVFMVIYILVYSVGNFYIDYRWFSIYNHLDIFWIL
                     FFSKFNVQTLFWGLFVGLFILNFVLIRILGGKGRIFTKNILDRIRIPGFGTTRNLLFV
                     LLAAGVIVLGFFMGLWASAYWKEYLMYKNAVSFSGFPNDPLFNKDIGFYIFTLPFYKF
                     MFRWLLVSLSIITIFSFFFHILNNGIFSQDGKLEFSRFARAHLSTLMGIIVLLIAAGY
                     RLLSYDLLFNNRAKFFGAGYTDVHAKLLAYDICIVISVIAAILLFANIITRSFKLPVI
                     TLITLLPVYFIFGTIFPALQQRFIVDPNELEKESPYIKYNIEFTRLAFGLNAIKEMPF
                     ENAPTLTLRDLEKNKDVVNNIRLWDWRPLKQTYKQLQGLKPYYDFIDVDIVRYTVNNQ
                     KVALNISARELDNSKLTVSKNSWLNNHLIFTHGYGIVASRVDRITPEGLPELVLYDIP
                     PKYKIQIPVELPQIYYGEHNNSYIITNTRVTPGEFDYPYGDENKYTIYNGTGGDMLDS
                     VIKRAMYAIAFGDFNILISNVITNTSRIHFRRNIKEMINAITPFLYYDSDPYVVVAGG
                     KIYWIIDGYTSSNAFPYSTPVQLEYGESINYIRNSVKIVIDAYNGSITYYISDPDDPV
                     INAYSKIFKNLFLPIDKMPSELQVHLRYPEGLFNLQAQQLLRYHMTNINVFYNNEDLW
                     EIPLQIYENEKEYMHSYYLVTALPGEKATEFLLILPFTPAKKDNMIAFMVARCDMPYY
                     GQLLLYTLPKDKLNFGPMQIEARINQDAEISKQLTLWSQRGSRVIRGNMLVLPVKDSI
                     LFIEPLYLKAETSEMPELKRVIVSYRDKIVMEENLSMALSRLFTEGGGISFDYSSEYG
                     LKDLINKAYMHFIQAEEYQRQGNWAKYGEELKQLKDTLTALRNAR"
     gene            complement(22955..24289)
                     /locus_tag="PLV81_08065"
     CDS             complement(22955..24289)
                     /locus_tag="PLV81_08065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOM10094.1"
                     /translation="MAEQKFAKHEIFAILLIALMNLFLFADQNLMAPNLTQIAHDFGF
                     NDVQRDVMLGGRISFVFWVLGGLVTLAIGYLTDKISRRNLFLFVILVGEIPCLLTGFA
                     QNYNQLFWLRALTGIGIGGALPLTFSLIGDYFSHKNRAAAAAWIGLAQGLGIAVGQLM
                     AGFIGPVYGWRLPFILVAIPNFLLAIIFLLFVKEPQRGKTEESLKELIEEGIAYTGRI
                     NWKEYKNLFKIKTNILVFLQGIPGTVPWGVFFIFLNDFYSQDKGYSVQMATLIVMTVG
                     AAAIIGAFVGGLIGNKLYNIKPKYLPMLCGTSTLIGIIPMALLLNYNPQIIVPEPDPT
                     WPLIFGFVTGFTVTITGPNVRAILLNVNTPETRGSIFSLYNLTDDLGKGFGPVIISAL
                     IVWFGRIMAFNVANLFWLVCGLLLLVMIWTFPEDEAKLNAILKERAAQMKRN"
     gene            complement(24382..25062)
                     /locus_tag="PLV81_08070"
     CDS             complement(24382..25062)
                     /locus_tag="PLV81_08070"
                     /inference="COORDINATES: protein motif:HMM:NF016163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HOM10095.1"
                     /translation="MYYIGIDPSWSGKNYTAVVMCDDALQVIDTCYTNDIKKIISAIV
                     PFNDAIIGIDAPLIIKNATGHRRHEIEFLRVFSRFGLGLHAVNKKRYPYFFPEVLYKE
                     LHALGFSFNNNNIFEVYPHATIMTCFNNMKLLQYKSKYGVTMRRENIEKLYNYLAAVI
                     KCSDLFTGTIAQAKGKQLKKYEDYLDALVCAYTVYYCSRKNCYSFGDAENGILLVPSP
                     ECRKVVDT"
     gene            complement(25137..26816)
                     /locus_tag="PLV81_08075"
     CDS             complement(25137..26816)
                     /locus_tag="PLV81_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOM10096.1"
                     /translation="MEKVWLKSYAPGVPHSIKYDEITVVHGFERSAKEYPNKDALIFL
                     DAKISFKKMQSMVNKIANALLDMGVKPGDKVAMLMPNMPQIVIGSYAAWKIGAVVVMN
                     NPLYTDHELEYKLNNSESTVLITLDLLGPRMIALRPKTPVKKIIIAHIRDHLGFPKKQ
                     LLPIVAKDKHKDIPPTQDVHEWTDLLKKYPDKDPGTMAKFNDLANLQYTGGTTGVSKG
                     VMLTHKNLSCNLQQISAWFPGFKRGELTSLGILPYFHSFGLTTVMNLCLWQGWTQVLI
                     PRPEPGAILEAIVKHKVNFFPAVPTMYVGLLNHPKIKDTDISSIKGCFSGAAPLPVDV
                     IKEFEAKTGAQICEGYGLSETSPVATINPYGGKTIQGSIGLPVPDTELKIVDLEEGTK
                     EMPLGQEGEVCISGPQVMSGYYKMPDETAKTLRDGWLYTGDIGKMDENGYFYIVDRKK
                     DMIIAGGYNIYPRDIDEVLFEHPKIMEACAIGVPDKYRGETVKAFVVLKPGETMTEQE
                     VIDYCKTKLAAYKVPKMVEFIDSLPKSNVGKILRKELRKMELEKMQKEQAG"
     gene            27339..27884
                     /locus_tag="PLV81_08080"
     CDS             27339..27884
                     /locus_tag="PLV81_08080"
                     /inference="COORDINATES: protein motif:HMM:NF022586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HOM10097.1"
                     /translation="MKNKKVFSRTIDITCYEDEQDSVIIEGEIKDTRLCHYYLVTGER
                     KEPGILHNMKITMKVSGNSLTISDIHVEMIHYPREECIEANKNIQKLNGLSITKGFTK
                     AVKDIIGGTRGCTHVANCILAMAPAAVQGFWAHKAQKPVTKEFANQGSMSHYLVDTCY
                     VWRKDGPMIKKLSESINKIQS"
     gene            27927..>28126
                     /locus_tag="PLV81_08085"
     CDS             27927..>28126
                     /locus_tag="PLV81_08085"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOM10098.1"
                     /translation="MFGYHGKIVEVNLTTTSINVKNFDGDFARLYLGGNGFAAKILYD
                     NVKPDTDPLSEDNVVVFATGPFT"
BASE COUNT         8746 a         5218 c         4675 g         9387 t
ORIGIN      
        1 gattgtttac ggccaatgat acgtttttct atttcatcac gctttgcaac aagctcatgg
       61 atctgttgct tcaactcatt gcttttttca ttaaattcca caaggctatt ctgccactgt
      121 tcaatttctt ttgtgagatc atcaatctgc tgctgtattt tttgaatttc attttccagg
      181 tgagtctttt gcttggtatc ttcggtaatc tgccgttcta tagtttgtat atgcttatca
      241 atccatgctt tttcattttc gttctttgat aaatcttttt caacgcgagt tatcatgtta
      301 ttaacttctt caagctcttc ctgttcatta cggatactta tttctaactg gcttatatca
      361 tttcgtaact gttcaatatc agcacttgcc ttatgaatac ttgaatcaag ttgttctttt
      421 tgtgcatgga tacctgtttt atcaaacagt attgacctga aaccatcctc ataattttca
      481 aaaagctgta tatcatgttt taatgcaata aaatcaattt tttgcaacac ttcaattgct
      541 tcatgtacca gattgagcga aaggttgtgc aaagctttct gtaagttttc ctcggcacct
      601 gttaatgcag cgtggatgcg ttcacgcacc agctcacgct ctgcttcaga agactccaat
      661 tcatgtttac gtttttcaat ggcatcaacc agttgcctga taactatctc caacgattca
      721 cgtaatttct ttacttcagt ttcaaggtct ttaatggcat gttttttttg ttcaatggta
      781 tcgcgatagt tatggatatt ctgaattttc tttttacggt gtgcataaag gttttcaagc
      841 tgctctttat cactggcaat atttttaatg atagtgtggc tttgttcaat gtttttttct
      901 ttttcctcag taagtttttt aattgcctct tcaatttcct gaatgcgcag ctgagtggtt
      961 gcaattcgct gctgctgttg ctgtatcaat tgtttattac gagtagtttt accatcaatg
     1021 tcttctacct ttacagtata tgtatgcagt tttttatccc attcaaaaag ctgcagttga
     1081 atgtcatttt ttcttttttc atcgttctca ttttcagagg aaattaaagc aacctttttt
     1141 gataactcct cgcgttcatc aaaaattcgc tggcggtctt cagaaacctt ttgcagtttc
     1201 ttttgaaagt ccctgtaccg taaaatctgt aaagctatat cgttcttttt atatttttca
     1261 cgtaagctaa aatatatttt ggttttttct gcctgttttg acttaaaatc cttttcacgt
     1321 tcaatctctt ttataatatc attaagtcgt tcaaggttaa ctgtggtatc ttccagcttt
     1381 tttaatgatt cttttttctg aactttgaat cgtgaaatac cggcagcttc ttcaaaaata
     1441 tagcggcgat cctcagcctt agttgaaagt ataaggtcaa tcttgccctg ctccatgacc
     1501 gaataggatg ccttcccaag gccagtatca agaaacagtt cttcaatatc tttaagtcga
     1561 accggtgatt tatttatcat gtattcagat tcaccgtcac gaaaaaccct tcgtgtaact
     1621 gtcaccgtat cagaatcaat atttaaaatg cggttggtgt tattaattac cagcgatact
     1681 tcagctaatg acagaggttt tcgctgatcg gtgccagcaa atatgatatc ttccatttta
     1741 tcgccgcgga tattctttgc ctgcttttca cccaataccc actttatggc atcaactata
     1801 ttagatttgc cgcaaccgtt gggtccaact attgcggtaa tgcccttttc aaaattaatt
     1861 ttggttttgt cggcaaagga tttaaagcca aatatttcca atgaatctaa atacatcaag
     1921 aacacctttt aatattttgg attataaaaa aattgttgat tgtgcacgtt tagaatgcaa
     1981 ttagtttcat catgtcatta attgtgcaac tataattttt aaaaattata gggatatttt
     2041 cctgacatca tgtattattc ttaaaaagca gcttattagt tcagatagta tcggcagtac
     2101 gtattccatt atctatgatg cggagtttac gcatcacagg actacaaaac gtggtgataa
     2161 ctattacaca atggttgacc tcaacttctg gtatgataat gagtttgggt atgtgtgttc
     2221 attgtagcgc ctctattatc ttataacatg caggcgatag taactgtaca ttaaccttat
     2281 tctgttaaaa agggcggctt acatcagtca gccctttttt atgattgctg caattaaaaa
     2341 aatataattg aaagcataat ataatataag tatatttata aaatgaggaa tagatttgtg
     2401 aaagtatgtt atccaataat attgttaata tcaattattt ttattgcggg ttctacctat
     2461 gcaaaggagg tccattatac attagatgat agagatcgta taatacgaat ggaagtaaaa
     2521 ataaatgaaa tggacaaacg ctttgaacaa tactcattat atccatgtgt tgcattcagc
     2581 cttattgctc tgtttattgt tgcgtgatgt ctccctcatc tacacgatat ttcccgtaaa
     2641 gatactcctt tggtataaag cattgttttg atgacaattc tttatcatca ttgtatatga
     2701 gtataatgca tcatagctcc tttttagttt ttgttgttgg gacgcccaat ttaaagtgtg
     2761 tgctgtgtat gaggcaacat gttttctatt ttgtgttgca ctttattctt gaatatgcat
     2821 ttatatacct tttgtatttt taaagaactc ggattgtaag aattcaattt ctttacttaa
     2881 aaacgcaaca atacgtcact tcgacaggct aagtgtcatt acttatattg cttcgggtgg
     2941 ctatgataat gcacgccgct ttagtataac gcggcgtcat acgcggcgca cattggttgc
     3001 ttgcgcttcg gcacattttc acctccgctg cacttcggtt aaaacgtcac atacaccggg
     3061 gatgttatgc atactacttt tgcactattc atttaataaa gcattacagc tatctataca
     3121 ctcatcatat tgttttgcac atttaatgcg attaattcct ttttcttcaa cacatttatc
     3181 catctcctcc tgacattttc tatggcatat ttcacgtgca gaggttggtt tctttttctg
     3241 ttcagcagat attactattg ctacagaaaa aattaataca gctatcatgg ttgcgattaa
     3301 atattttgtt ttcattatgt aaatcatcct tatattttgt aatttctttc aaagataata
     3361 tttagtataa taattgcaat aaaaaaatta tttttataaa atactttaat tttgccaata
     3421 ctgatatatt tcagttacaa taatatatac tatacaccat taaattataa caatattggt
     3481 acacagatat tctcatttcc cgcttttcac tgctgtagta cacaaatggt aacctggatt
     3541 ttgccttata atgtttaaga ataaatagtg tttaaaaaat atttttatat tgaattattg
     3601 ttgaattatc tatattatta ctatataata tttttttaac taaacaatgg tataaataat
     3661 atgatacaaa aattattcat agcactgata tcaattatag tagtatcaag tataactgcc
     3721 tgtgatcgtc tgaacatgta tgacattgca agtagaaaat caagaacagc ttatgctctc
     3781 atttttaatg gcacaacata tagattggca atagcccatc ctgaatatga taaaatttat
     3841 accagtaatt ttgtattttc agcaggctca gagggagtgt cagccaataa tcttacaatt
     3901 atagtatatg acagcaccaa tacctacatt tctaataacg atttaacatc gtggnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgaact gatgcaatct tttcaaagta
     4081 ctcctccaac gtctggaaca tatctgggtg gttttagaac taaaaattat ttttatgtat
     4141 ggtataacgg tggaaatgat tctatcttta gaactaatgg tagtgtagat ataaattata
     4201 atgacactac tgcatggagc aatgtcgttg atgttgcaat tactgatgat gataaaatgt
     4261 atgctataat attcgttagc ggaacatata atctgttaca tataaaaggg ccaatggact
     4321 ttaccacggt attgttcctg ggaactacag gaacatttaa gatagattgt cttgacagca
     4381 aacacattat agttgctgct tatggaaata ctgatggtta caatggactt tttatctata
     4441 atgttgaaag caaaaagatt gaaaaattca taactgctga ggatgctatt gcactttatg
     4501 ttcacagatg aaataaacaa tagttataat tattttttta catgaaacgt tttgatatta
     4561 tactactcaa ttttttaatt taaaaaaaat ggacataata taccatggtg tatagtagta
     4621 ttatctaacg ataaactttc aatgaaaggt aaaatggttt aaaacataat gcatgtattt
     4681 ttaatacagt acaggtattc tgtggtacaa ccaagagtgt atgataattt caaagagatt
     4741 attactggtt atttatgata caattgcgta gttttgttat attatctggc ttgcttatat
     4801 ctctaccatt tatttcatgc tcaaccatac agttagctca acaaaaaaca cagctatatt
     4861 ccactacgtc agatacactc acattgccca aaagcaaaga acgcattgat aaaactccaa
     4921 cgtacgaact tttttttgag cttcttgata acacccgttg tcttgatatt tctgttattg
     4981 cacaggacag aaacggcaaa cgatttcgct gtgcatttat actggaagca ttagttgata
     5041 catcgttata tcctgtaaaa ccacttaagc caatatatac tgaatttgcc agaaattttg
     5101 atataaactg gcgcttccaa agcaatattt tcatatgcag tgatgaaaat gatccattga
     5161 aaagacttac taaagggaac tatcgcctga aaatatcagc attctcaaat aattattata
     5221 cattcactat cgatataaaa tcatcggtgc ctgtagaatt cagaaaataa aatttgttat
     5281 gttaactcag ataggtacta tatgccaaag cattatgatg acattgaatc tgaagacctg
     5341 tatgaaatgg cattacaatg ggcttcacag ggattttacg ataaagctat tgagcttgta
     5401 aagcaatcaa tagaactcaa tccaaatttt attggtgcat atattgattt agcgcattat
     5461 tacgcaaaaa aacacaggta taacgatgca ttccatgtat taaaaaaagc ctcaaagatt
     5521 gatatgtgct ttcaccgttt aaattacctt atggcaaaat atgcatacaa aaatggcgat
     5581 tatattgcag catcaaaata tattgatcgg gcaatagaac tttcggctga aaaattgtat
     5641 attaaagcaa aagccgttat tggtaaaaaa agtgttaaaa aacttacaaa aaaaaaacag
     5701 taataattca taaaattatt tcttattgta aaataatgaa ttatatattc tatgtattca
     5761 gcaatatata tattttcttg tagaactaaa ttcatgtttt aaaatgttta ttttataaca
     5821 caaattttac ttgcactttt aactatataa ccttttacta tatatattgt aaaataacta
     5881 tttcaggagg ttatatgaaa aagatattac ttgtgttact ggtatttttt acagcttcca
     5941 gtgtttttgc tgctggcata ggcatcaaag gtggatactc aaagatgtat gatgactatg
     6001 cagatgcatt tgatgacaca tggaatattg gtattgtttt tgatcttggt acatttttat
     6061 ttaataatct gcagttcagg ccgggtattg attacctgtc actggaaaat gaagatgcca
     6121 attttgaata tgctgatgtg tatggcattc atctagactg gtactggttc tttatgggca
     6181 aaaaacaatt ttcaccattc ttagggtttg gtccagcgtt gaactactac agttttgatg
     6241 atgacagaac caataatgat gattcagacg caggtattga aggatttgca ggtgttgatt
     6301 ttgatttaag tggtccatgg aaacttatgc ttgaattgcg gtatttatgg catgacattg
     6361 ctgacagaga cacaactatt gtcaaactaa atgttggttt gttatactat ttttaattat
     6421 taaaaaaaag aggcggttat ctaccgcctc ttttaatttt actccaaccg ttttttatca
     6481 aaatcagaac gtttaaataa cgatagtact gtaatccctt tttcttctat tgcttcacgt
     6541 ccaccttctt cgcggtcaat aataactgct gcggcaatga cgctgagccc tgattcagtc
     6601 aaccgttcaa ttgcagtgat ggtggaacca ccggtggtga ttacatcatc aatggcaatg
     6661 acgtttttta cattagccgt attgccttct atccactttt gagtgccatg tttctttggt
     6721 tctttcctga caattagtgg atacaaaacc ttgccctgag caaacgcatg catagccgtt
     6781 gacaatgaaa tgggatcggc acctaatgtt aacccaccaa ttgcatcagc tggtaaatcc
     6841 tttattaatt caaacatgca cctgcctact aaaatacaac cttcaggatc aagtgttgtt
     6901 gccttgcagt caaagtaata aggactttgt ttgccactaa ccagtgtaaa tggcggattg
     6961 tccctgtaca taaatgactt ctggtataaa atttcaaata atctttggtt atcgttcatt
     7021 cacctttctc cttcttgtac ttttttggcc acagtctggt aagccgttct tttatattct
     7081 tttcattccc ttcttcagaa ggtgcgtaaa aaatcaaatc ttttatttct ttgggaagat
     7141 attcttcatc aacaaaatgt cctggataat cgtgaggata tttatagcct tttgcatatc
     7201 ccagctgctt catgagttgt gtgggagcat tacgcaggta gagcggaatt tcatgtacat
     7261 cacttgacgc aatttctttg gcattaacca gtgccatgta acttgcatta cttttaggac
     7321 acgatgctaa aaatgttgcc atgtgggcaa gtataatttc acactcaggc aagccaacat
     7381 ttttcacagc cagcatccca cttgttgcaa cagtaagcgc cagtggatac gcattcccta
     7441 catcctcaga agcaaatatt accatacgcc gtgcaataaa ctcagggtct tcccctgcaa
     7501 caagcatgcg tgccaggtaa tacaccgcag cgttggggtc agagccacgt aatgatttta
     7561 taaacgccga aatagtatcg taatgcttat cgccagccct gtcatatgca atgctctgtt
     7621 ccttgtaggc ttcaaaaact gtatccttat caattgtgcc ctgccctttc agaaatgccg
     7681 cagcctcaag tatattcagt gcacgtcggg catcacccat tgcaagctgt accagtgcat
     7741 ccagtgcagc ttcatcaagc gttatatttt tgtgcgatag ttcctggtca ttggctattg
     7801 ctgcagtaac tatcgcccgc aaatcatcct cctgcaatgg atacagccgc aatacgcggg
     7861 ttcgtgacaa taatggcgct atgatgctga aagacggatt ttccgttgtt gccccaatca
     7921 gaacaatgtc accattttct actgcaccaa gaacggcatc ctgttgagcc ttgttaaagc
     7981 gatgaatttc atcaataaaa aggagtgttt gaacacctgc agttctattg gccttcccct
     8041 tatcaataac cttacgtacg tcagctaccc cggccgagat agcgcttata aaatatgaat
     8101 ccatattaca ggcactggca atgagatgag ccagagtggt tttgccggtt ccaggatcgc
     8161 cccacaggat aagcgacaga gctttctttt gttcaaagat agaatacagt aacttccctt
     8221 ttgaaagcaa atgcttttgc cccacaaact cttcaatagt tttggggcgc atccgctctg
     8281 ctaagggaat gtatgtgttt ttatcaaata agttcattat ccataactta catactgcgc
     8341 ctgtacatac caccaacgct atacagggca ccggttaact ggcctaatga acaatatttt
     8401 gcaacatcca ttaatgtttc aaacacattt tcatgttgtt tcacagcagt ttttaatttt
     8461 tctaaagcta ctgttgattc ctgtttccat acagcatgaa atgcttccag tgaagcaagc
     8521 tgagtatctt tttcttcagg agttgcccgt accacttcat caggaatttg aataggactt
     8581 ccgtttttag aaaggaatgt attaacgcca atgataggca actctcctga cattttttta
     8641 ttttcatagt acagggattc ttcctgtatt ttgttacgct gatacattgt ttccatagca
     8701 cccagtacgc caccacgagc acttatattg tcaaattcag caataacagc ttcttcaact
     8761 aaattggtca attcttcaat gataaaagaa ccctgcagtg gattttgatt ttttgcaagt
     8821 ccatattcat gattaataat catctgtatt gctagtgctc ttcgtactga ttcttcagtg
     8881 ggtgtggtaa tggcttcatc atacgaatta gtatgcagtg agttgcagtt atcgtatatt
     8941 gcatacaggg cctgcaatgt ggtgcggata tcgttaaatt caatctcctg tgaatgcagg
     9001 gatcggccag aagtctgtat atggtatttc agcaattgcg agcgctcatt agcaccgtat
     9061 ttatatttca tggcttttgc ccatatcctg cgagcaactc ttccaataac ggaatattca
     9121 ggatcaagcc catttgaaaa gaaaaacgat aaattgggag caaaatcatc aatgtgtaaa
     9181 ccacgtgcta aatagtactc cacatacgta aaaccatttg ccagtgtaaa tgctaattgc
     9241 gttattggat ttgcacctgc ttcggcaata tgatatccag aaatagaaac tgaataaaaa
     9301 ttattgatct tatgtttaac aaaatattcc tgcatatcac ccataagttt taaagcaaag
     9361 tcaactgaaa atatacaggt attctgtgcc tgttcctctt tcagtatgtc agcctgtaca
     9421 gtgccacgca cggtcctgag tgtatgattc ttaatgttgt ggtatacttc agctggtaca
     9481 accatatcac cgggaatacc cagaagcagt agccccaatc cattgtgtcc atcaggcaat
     9541 tgtgctgctc tatatactgg tggtgtagca ttacgctttt gatataattc ctgtattttt
     9601 ctttttactt catctgttaa gccattttgc tgaatatata attcacattc ctgatctatt
     9661 gcggcattaa aaaaatatgc agccataatt ggtgcaggcc cattaatggt catacttacc
     9721 gatgtattgg gatcagtcag cttaaatcct gaatataaac gttttacatc atcgaccgtt
     9781 gaaatcgata cacctgaatt gccaattttg ccatagatat caggacgctt atcaggatta
     9841 caaccatata atgtaactga atcaaatgca gttgaaagcc gcaccgccgg catagtatac
     9901 gaaagataat ggaatcgctt attggtacgt tcggcaatac cttcacctgc aaacattcgg
     9961 gttgggtttt catcttcacg cttgaaagga tacacacctg cagtgtacgg aaattcaccc
    10021 ggcacatttt cctgcatact ccattcaata atatcgcccc agtttttaaa ttttggcata
    10081 cacacttttg gaatttttgt gtgcgatagt gactgtgtaa aagcctttac cttaatttct
    10141 ctaccgcgaa ctttgtatgt ataatattca ccctgataat ttttcttttt atcttcccat
    10201 tgctgcaaaa tctttttact ttcttcatct aatgacgaat atgcttcgtt atacttttga
    10261 tgtaaatctt tcaaaatttt tgcatcacca ccatgctgtt cataatattc aatactgttt
    10321 tttaaactga aacaatattg tgcttttagt gattcttcct gcactttatt attgtatttt
    10381 cgtattgttt cagcaatttc agaaagatac cggctacgtt cgggtggaac tatatacaga
    10441 atgtcttttt tttgtttatc tgcagtatat actgcaaatt gtgttttact ttttgcgttt
    10501 attgtatgta ttaaatgatt gaacaataca ttgactccat catcattaaa ttgacttgcg
    10561 atagttccaa acaccggcat ttcatcaggt gaaatatcaa aggcttcttt gttacgctgt
    10621 acctgttttt ttacatcacg caatgcatcc agcgctccca gtttatcaaa tttattaatg
    10681 accacaatat cggcataatc aagcatatca attttttcca gttgtgttgc tgcaccatac
    10741 tcgggtgtca taacatacat ggatatatcg ctgtattcca gcacatcggt ctgtgcctgt
    10801 ccaatacctg atgtttcaag tagaatcaaa tcataccctg ccagtttcat aatgtgtagt
    10861 gcatcgctaa tagtgtttga aaccgaagtc tttgagccac gtgttgcaag tgagcgcata
    10921 tatacattgg ggtgatagat agcattcatt cggattctat cgcctaaaag tgcaccacct
    10981 gttttttgcc gggttggatc aaccgaaagt atggctattg ttttatcagg attggactgt
    11041 tgaaatcgta ggaccagttc atcaataagt gatgatttcc cgctgccacc agttccggta
    11101 atccccacaa caagcgtgct atcatcagca gttaattgtt gcacggtagc aatatcatct
    11161 tttacaagct ccttaaaatt ttcacaggca gagattgcgg ttgctatcgc ccgtatattc
    11221 ttttttttaa ttccttctat atcaaaatta ttaatattac caacaggata atcacttttg
    11281 gaaagcaggt cattaatcat tccctgtagc cccattgccc tgccatcatc aggggaatag
    11341 attttgcaaa taccgtatgc ttcaagctct ttaatttcat gaggcaaaat tgtgccccca
    11401 ccacctgcaa agattttaat tgaggctgca ttgtgttctt tgagtaaatc atacatatac
    11461 tgtaaaaact caacgtgtcc accctggtat gaggtaaccg caattgcctg aacatcctct
    11521 tccagtgcgc tttttacaat ttcccacact gatcggttat gaccaaggtg aatgacttcg
    11581 gcacctgttc caatcatgat gcgccggaaa atattaatgg aagcatcatg cccatcaaat
    11641 agcgatgcag cggttacaat ccgcacatga tttttgagct tatatttttg cataaattca
    11701 ggatgtgtca tacaacggca acccccatat cactttttgt gcattattat aatatatatt
    11761 acaatattac aataataatt tctataggga aaggattttg tgaaaatatt taaaattaaa
    11821 gccagtgaag gttctatttc agagtatgat tatgaaaaca ggatactgga aacactcaga
    11881 caattgggga ttaaagctga agaatgacat tatgtatgaa ctttaaattt attgctgatc
    11941 atgtaatata aaaccttcat ttaacataca atttcgaata tcagcatttt tcataaggga
    12001 ataccttccc ttatagcata ataggttaag tatgctatat tattttctct ggtttttttt
    12061 atattatatg attgtataat caaatctgaa gaaatatcta attttactaa tcgccttcgt
    12121 attaacattg tgatttcttt tttacgtgga aagtcaattt catcatcaac tattataaga
    12181 aaatcccagt cactatcagg tttttctcta tctttagcac ggcttccaaa taaataaact
    12241 tcaagtacgg tataaccaaa tttttgcaat tcttcaacta ttatagattt agctgctttt
    12301 aaaatttctt ctttcgtcat cattattacc atgttacaaa tttttgtatt acactacata
    12361 gctcttatca atttaatata taataactta acacttgtca accaatttta tttagagccc
    12421 tttgagtatc aattataaac aaaaaatccg gcaattgccg gattttttac tggcttcacg
    12481 tatgtagttt agcattactc gtcctgtgaa gatccacctc tgccagatgg ttttgcaaca
    12541 aaacggcgtt gttttgtctg cgtttcaaat cttctgatct ttttcaaacg gcgtctttcg
    12601 gcttcttcct ttttgcgctt cttttcttcg cttggctttt catagtatcg gcgccttttt
    12661 agctccttaa agataccttc aaaagccatg cgttttttca gatttttaaa cgcacgctcc
    12721 aaatcaccgt ctgttacctc aacttctaaa ggtactggca ttttgtaatc acatcccttc
    12781 taattattat taatgatttt tttaagactt taacaatatt aatatagcaa tataattgtc
    12841 aagataaaaa atatatttca ttatatgctg gcgtaaacaa tgagattgtt gacgcttgcg
    12901 cttgcaatga cgtgccctca ttgtcattgc gaggcacgca gtgccgaagc aatcttttct
    12961 gtaccatatt ccgggattgc ttcgcttgcg ctcgcaatga cttaataaat acatccatat
    13021 atacaggagc tttataaact ctaaataatc taaataatac tttacaacgt tactatacta
    13081 tcaatcatat taattaaaat tttattataa acattgctat atgtcaaaaa aaatcattta
    13141 cacaatactt tccttgctca taaccatttg cttacattgt agtaagcagg tgttacaatc
    13201 accacctcca gaacagtcac aaatccctga tatacagtcc acaaatatta tactggaatg
    13261 cctgcagaaa tcaaataatg gctatccctt tgttgaacta tcgctgatta tagaaaatac
    13321 tggccgttat tttgtagcaa cagtatatgg tcagatttat cagatataca gtaagccacc
    13381 tgtatttgcc ccaccttatg caacatgtgg tgtttctata aaaaataata atacaattca
    13441 ttattttttt gtatatgcta atgcaacaac agatatcatt gcagtacaat cactggttga
    13501 aaaagtatca gataattata ttaacactat ggattatcaa acaattataa agattccagt
    13561 acaattacat aatcgtattc aatcaactgt tactgtaaaa taatattcca tgtgctcgta
    13621 tgtcaccaac acaaaaaatg attgccctta caatgttttg tggtactatg cagtttaatt
    13681 tcatgatgga tgtcattcta tggtcatagg tcttgatata ggcggcacaa atattaaagg
    13741 cgttattgtc gatagcaccg gcgttatacg tgcacaaacg tcggttccca caggaaaagc
    13801 tatattggaa actgaacaat atattataca gtgcattacc attttacagg aactatcgca
    13861 cacaaataac aatgaaattg aggcgatagg cattggctca gcaggcgcta ttgataaaca
    13921 caaaggtatt gtaatcacaa gtcccaatat tccatggtgg aaaaattatc ctgtggtgca
    13981 acaaatacag aaattatcag gatgtaaaac ttttcttgag aatgatgcaa actgcgctgt
    14041 gtttggcgaa ctgtggcttg gcaatggcaa acattttaaa aactggataa tgcttacgct
    14101 gggtacgggc attggaggtg gtgcggttat tgacggcgtg ccctacactg gccgggctgg
    14161 cagtgcaatg gaatttggcc atacaactat tgattataat gggctgctgt gtaaatgcgg
    14221 aagaaaaggc tgccttgaat gctatggttc agcaactgcg gttgtcaatt ttacaaaaag
    14281 tcatataagc atgtatcctg attcaacaat tacaaaacgc atgacacagg agtcattaac
    14341 agcacaattg gtgtttgaag aagcacaaaa aggggatgct ctggcattgt atatttttaa
    14401 tttaattgga caataccttg gaattggtat tgcaaattta gtgaatatat tcaatcctga
    14461 ggcagtgata cttggtggag gattatcagg cgcacatgaa ttttttatgc cacacattat
    14521 tcaaggtgta caaacgctgg ccttaccagg catgaaggaa gatattcact attgtgtaac
    14581 gtcatacggt aattacacag cagcaatagg cgcagcaaaa tatgcgctgg ataatctttt
    14641 agaataatac atattttaga ggtgaacatt atgtttttta ccaaccagtt acttttcagt
    14701 gaacaaacgt taaaccagat acatctatat gctgggaaat ggcttgattt tattatggtg
    14761 gcttttactc agttaggcaa tgagatgttt tatattcttg tgataccatt tttattctgg
    14821 tgtgtcaata aacgcattgc aaccattgtt ggtattgcat ttttactatc tgccgcaatt
    14881 aacgatattg caaaattttt atttatcaat ccaaggccgg atgctgcaaa ccttgctcca
    14941 caaatagcag aattaaaccg tatgtattgt cccgttaata gccctggatt ccccagtggt
    15001 catgctcaaa gcgcagtagt gttctgggga acattggcct atgttgttaa gcatcgatta
    15061 tttaccatat tttcattatt tttgattatt gcgatttcat attcacgact gtatttaggt
    15121 gtacattttt taggcgatgt tgctggtggt ataatcttag gcactgttat ttttctatgt
    15181 gttattgccc tgataccaca ctatgacagg ttatggaata cagggataat tcctgcagtt
    15241 gtcttttttg tcatactacc ttttgttatc gcaattgttt taacaggaaa tgaaattgcc
    15301 aaatcattgg gtgtactatc aggttttgca attggcttta tactcaacaa tgataaatcc
    15361 gaagagatta ctttttcaat tgtacctgca cttgttaaat ttgtcattgg gattaccatt
    15421 attcttggta tcaaagaagg tcttaaaata gtgtttccat catctaatgt atttgacttt
    15481 atacggtact ggtttatggg gttatgggta tcgtatggcg cacctgcact atttatgaaa
    15541 attccgtacc tttctaaaaa atcggaataa tgcgctgatt aattatatct cacagcatca
    15601 acagtaaagt taacatatag ttaatttaca taaaaattca tagtaatatt gttgactttt
    15661 tttaagcggt tcttttgtaa tacaaatcta aaaattattt accggtaact atcgccaaaa
    15721 taataatttt tttgggcgat agttcatagt aaataaagat ttgtaattag tcaggtgatg
    15781 tatatgaaaa agcagcttca ggacctctat aaacaaacaa tatccgcaat tgacaaagcc
    15841 acaacgatag atgaactgga acagttgcga gtcaaagtcc tgggacgcaa aggcgatttg
    15901 actcagattt tgcgctccct tggcacattg ccacaggaag aacgtgttga gattggcaaa
    15961 ctatccaacg aactgaaaga aaaaattgaa tctgctctgg aaaatcgcaa aaccgtttta
    16021 accgaacaac aaataaacaa gattatcatg gaagactggc tggatgttac gcttaccatg
    16081 ccaggcacat taaaacatta tgacagaggt catctgcatc ctatttcgca aatacagtat
    16141 gaaattgagg acatatttac ctctatgggt tttgaaattc ttgatggtcc tgaagtagaa
    16201 actgattact ataactttga agcactcaac attccgccgt atcaccctgc ccgcgatatg
    16261 caggatacat tctggactga agatggcaat ctgctcagaa cacatacatc agcaattcag
    16321 atacgaggta tggaacggct taaaccgcca tttagagtca ttggtcctgg cagggttttc
    16381 cgctatgaaa gtattgacgc ctcacatgaa aatacatttt atcaggttga aggaatgatg
    16441 gtggataagg atatctctgt agcgcacctt atctatttta tgaaaacatt acttaaagaa
    16501 atcttccaga aagaaaccac catacgcctg cgcccaggat atttcccctt tgttgaacct
    16561 ggatttgagc ttgatattca ctgccttata tgtggtggca aaggttgcag tgtgtgtaaa
    16621 tattccggat gggttgaact gttgccatgt gggttggttc atccaaatgt actcaggtat
    16681 ggcaatattg accctgataa gtggtcagga tttgcatttg ggctgggttt aaacaggctt
    16741 gtcatgatgc gctatggcat taatgatatt cgccatttct taagcggcga tattcgtttc
    16801 ctctatcaat tttaagtctt gtgtggagtt agcattatgt ggatttcatt aaatattatt
    16861 caggatatgg ttgatattaa agacattcca gtggctgata tagccaaccg gcttaccatg
    16921 tcaaccgctg agattgattc tattgaatat tcccatgaac ttcttaaaaa tatttatact
    16981 gcaaagattc ttgaagttgc caaacatccc aatgctgata aacttactgt atgtaaagtg
    17041 gataccggca agggtgtgtt acaggtagta tgtggtgcac caaatcacaa agctggtgat
    17101 attgtagcac ttgcaccggt gggcaccaaa tttactgaaa cattttccgt tacaaaagca
    17161 aaactcagag gtgttgaatc cgaaggaatg ctgtgttcgg aacgtgaact gggaatatcc
    17221 gatgatcact cgggtattat ggtatttcca caaaatactc ccattggcgt taccttagca
    17281 caactgtacc ctaaccttat agatgttcgt ttagatatag acaataaatc aattacccac
    17341 aggccagatt tatggagcca tacaggtttt gcccgtgaga ttgctgcgtt atttaataag
    17401 ccatttaaat atccggtaaa ctatgatctg gcaaaggatt ttaaatctaa agaaacacta
    17461 aaagttacta ttaaaaatcc tgaaggtgca ccccgttatt gcggattgat ggtaaataac
    17521 ataaccataa aggaatcacc tgactggctt aaagcacggg ttgaagcaat tggtatgcgc
    17581 cccattaaca acattgtgga tatcactaat tatgttatgg ctgaattggg cgaaccaatg
    17641 cacgcctttg accgttcaaa gctgcgtggc aatgaaatca tcgtgcgatt tgctgaaaat
    17701 ggtgaacatt taactacact ggatggacag gagcatgtat taacttctga ggatgttgta
    17761 atagccgata gtggcggtgc tatcgcccta gctggtgtta tgggtggtgg caacagtgaa
    17821 atagaagaca acacaacttc aattgtactt gaagctgcaa attttaatcc tgtttatata
    17881 cgccgtactg caaacagata caatcttcgt actgaagcag ctatacgttt tgaaaaatca
    17941 ctggatccag aactatgccc tgctgcaatt atacgatgct atgaactgat aaagatgctt
    18001 tgtcctgaag ctgaagcagt aacaccaatt attgatgcct atcctgtacc tgcaaaaccg
    18061 gtcagtattt caaccacaac cgattttatc cgaagaaaat taggccattc aattgatgac
    18121 aacactatag tatcaattct tacatcatta gattttaagg taactaaaaa taataataat
    18181 ttaattatcg atgtcccaag ttatcgtgca acaaaagaca tttcaattcc tgatgatata
    18241 gttgaagaaa ttggcaggat atacggatat gataatattc ctgaacaacc accgtttgtg
    18301 ccctgtgcgc caccctcacg caacattaaa cgtacgtttg aaagagaaat taagtcaatt
    18361 ctggttaatg atttccatat gactgaagtt agcaattatt cattcgttgg cgaagcatta
    18421 ctcaataaac tacaattaaa cgaaaacaaa gaattgcaat tgaaaaatcc gttatcaaaa
    18481 gaacaggacc gtttgcgaag aagccttatt cctaatgtaa ttgtaaatat aacaacaaac
    18541 cagcgcttct ttgatgattt cagaatattt gaattgggca ggatttacct taaagaagat
    18601 agactatcaa aagatttagc taaggaaaat accagggtta ctggtgttat atattcaaaa
    18661 aaaactgcat atcctgtttt ttatgatgcc aaagcctgtt gcataggggt ttttgataaa
    18721 acatttcgga acaactatat tttgaagcca aaaagtgata atctgccacc atatgcacat
    18781 ccagtacgtt cactggaact tttaatacat aacaaacctg ttgcactggt atgtgaactt
    18841 catcctaaaa tagctaatga atttgaaatt aaaggacagg ttgcactttt tgacattgat
    18901 gttgatctgc tgttatcttt gccacagcag gatatagtgt ttaacgaact accaaaattt
    18961 cctgatgttc cttttgatat ttctgtactg actgatgaaa aagaatatgc cgctactctg
    19021 gtggatatgc tatataaagc tgacaaagaa catattgctg atattgaagt tatctccgta
    19081 tataatcaac caccaatacc acagggcaaa aaatcagttt cgttaagaat aacattcagg
    19141 gccaaagacc atacgttatc acctgaagaa attgacaatc ttcagaagaa ggtgattgca
    19201 tttgtagaaa aacatggata taagttgaga taatgtatta aaatacacac tgccccacta
    19261 cggatgggtc agtcataata ctatagattt tcataattta ggcttgtatg gatgtggtat
    19321 aaaaatctta tacacatacg ataagccatt ctgcaccaca tcttctattt cacattttaa
    19381 aactatatca ttcccattat tatcgtaaac aatcacgtca atataatcat gaatgtttaa
    19441 tgttttgctt gattttacag ggtaaaattt ccctattcgc ataatcatta ttcatttata
    19501 aattctcttt cccatgggta atgggctctg ttctgtttca atgctgtttc ttctttttta
    19561 aacattgtaa attcatccca taaatcctga gtaaaatatt ttggcttaat aaataactca
    19621 acctctcctt tttgcaattc atagttgcta tcaacatgct gaaataaatg gttccaggat
    19681 acaactttaa accgtaatgg caaaattata ttttgagtta tttcattggt tttatgctta
    19741 acggaaaagt tattgggatt ataattttca gggctttcat agataacttt cataatctta
    19801 tttttcttat caataataag acctataact tcaatatcat gtttaccaaa cataattttt
    19861 gcagctttaa tccaccggta taaatgtttc gacttagcca tggcacaaaa cctttagtat
    19921 tattgacttc acgttcccag aaataatgat acgcaatata ggtatttcct ttatcatcca
    19981 cactggcccg gtaatataca gcgtatggaa actcatattc agaatttgaa ataatatatg
    20041 gacaatattt atctgctaat atatcatttt taattggtgt aaaaccaggt atatcagttt
    20101 catttacacg tacggtttta tttgcaacat acgttacaaa tccaataagt attacaagca
    20161 aaataacaat aactgatatt ataatttttt tcatgatata ctccctctat cttataataa
    20221 atattacctg gcattacgta aagctgtcag cgtgtctttc aattgtttta actcttcacc
    20281 atattttgcc cagttaccct gtcgctgata ttcttcagcc tggataaaat gcatatatgc
    20341 cttattgatg agatctttca acccatattc tgatgaataa tcaaaactta ttccaccacc
    20401 ttcggtaaat agtcgtgaaa gtgccattga aagattttct tccatgacaa ttttatctcg
    20461 atatgaaaca ataactcttt ttaactccgg catttcgctt gtttcagctt taagatataa
    20521 tggttcaata aaaaggattg aatccttaac aggcagtacc agcatattgc cacgaattac
    20581 ccgtgaacca cgttgactcc acaatgttag ctgtttagaa atttctgcat cctgatttat
    20641 tcgtgcttca atctgcatag gcccaaagtt aagtttgtcc tttgggagtg tgtacagtag
    20701 tagttggcca taataaggca tatcacaacg ggcaaccata aatgcaatca tattgtcttt
    20761 cttggcaggt gtaaacggca atattaaaag aaattctgtt gctttttctc ctggtaaagc
    20821 agttaccaga tagtaactat gcatatattc tttttcattt tcatatattt gcaatggaat
    20881 ttcccacagg tcctcattat tataaaaaac atttatattt gtcatgtgat atcgcaacag
    20941 ttgctgtgct tgaagattga ataaaccttc aggataacgc aggtgtacct gcaattctga
    21001 aggcatttta tcaatgggaa gaaataaatt tttaaaaatt ttggaatatg cattaatcac
    21061 aggatcatct ggatcagaga tataatatgt aatgctaccg ttatatgcat caatgacaat
    21121 ttttactgaa ttacgtatgt aatttattga ttcaccatat tccagttgta ctggtgttga
    21181 atacggaaat gcatttgaag atgtgtatcc atcaataatc cagtatattt tacctcctgc
    21241 aactacaacg tatgggtcag aatcataata taagaatgga gtgatggcat ttatcatctc
    21301 tttaatattt ctacgaaaat gtattctgct ggtattggtt attacattag aaattaagat
    21361 attaaagtca ccaaaagcta ttgcatacat agcccgttta ataacagaat caagcatatc
    21421 accgcctgtt ccattatata ttgtatattt attttcatca ccatacggat agtcaaattc
    21481 accaggagtt acccttgtat tagtaataat atacgaatta ttatgttcac cataatatat
    21541 ttgtggcaat tcgacgggta tctggatctt atattttggt gggatatcat acagtacgag
    21601 ttcaggaagg ccttcaggtg taattctgtc tactctgctt gcaacaatac cataaccatg
    21661 agtaaaaatc aaatgattgt ttaaccatga attttttgaa acagttaatt tactgttatc
    21721 taattccctt gcggaaatat taagtgcaac tttctgattg tttacagtat accgtactat
    21781 atcaacatca ataaaatcat aataaggttt taacccctgt aattgcttat atgtttgttt
    21841 taatgggcgc cagtcccaga gacgaatgtt attgaccacg tccttgtttt tttctaaatc
    21901 ccgtagtgtc aacgttggcg cattttcaaa tggcatctct ttgattgcat tgagcccaaa
    21961 agctaaccgt gtgaattcaa tattatattt aatatatggt gattcttttt ctaattcatt
    22021 tggatcaaca ataaaacgtt gctgcaacgc aggaaagatg gttccaaata taaaataaac
    22081 tggcagtaag gttattaaag taataactgg caatttaaag ctgcgggtaa ttatattggc
    22141 aaaaagcaaa attgcagcaa ttactgatat aacaatgcaa atgtcatagg caagcaactt
    22201 tgcatgaaca tcggtatacc ctgcaccaaa aaactttgcc ctgttattaa acaataaatc
    22261 atatgatagc aatcggtagc cggctgctat cagtaatact attatcccca taagcgtaga
    22321 aagatgtgcc cgtgcaaaac gggaaaactc cagtttccca tcctgtgaaa aaatgccatt
    22381 atttaatata tgaaagaaaa acgagaatat agttataatt gatagtgaaa caagtaacca
    22441 ccgaaacatg aatttataga acggaagagt aaatatataa aatccaatgt ctttattaaa
    22501 tagcggatcg tttgggaagc cactaaaaga aactgcattt ttatacatta aatattcttt
    22561 ccagtaagcc gaagcccata aacccatgaa aaaacctaaa actattacac ccgcagctaa
    22621 caacacaaac aacaggttgc gggtggtacc aaatcctgga atacgtattc tatccagtat
    22681 gttttttgta aatattcttc ctttgccacc cagaatcctt attagaacaa aattaagtat
    22741 gaataatcct acgaataaac cccagaataa tgtctgcaca ttaaactttg aaaagaagag
    22801 aatccagaat atatccagat ggttatatat tgaaaaccag cgataatcaa tataaaagtt
    22861 acccactgaa tataccagta tatagataac cataaaaaca atcagtgcta ttaagaactt
    22921 tttattcatg gattacccca ttattgtttt atcattaatt ccttttcatc tgagcagcgc
    22981 gttcttttaa tattgcattg agtttagctt catcttccgg gaatgtccat atcattacca
    23041 gtaataataa tccacacaca agccagaaaa gatttgcaac attaaatgcc ataatccttc
    23101 caaaccaaac aatcagtgca ctgatgataa ccggtccaaa tcctttacca agatcatcag
    23161 tgagattgta aagtgagaat attgaaccac gcgtttcagg agtattaaca ttgagtaata
    23221 ttgctcttac attaggcccg gtaatagtta ctgtgaatcc agttacaaat ccaaaaataa
    23281 gtggccaggt tggatcaggt tcgggaacta ttatttgtgg attataatta agcaacaatg
    23341 ccattggtat tattcctata agcgttgatg tgccacaaag cattggcaaa tattttggtt
    23401 taatattata cagtttgttc ccaataagtc caccaacaaa tgcaccaatt attgcagcag
    23461 caccaaccgt cataacaatg agtgtagcca tctgaactga atatccttta tcctgtgaat
    23521 aaaaatcatt taaaaatata aagaatacac cccatggaac agtgcccggt atcccctgta
    23581 aaaagacaag gatattggtt ttaatcttaa ataaattttt atattctttc cagttgatac
    23641 ggccagtata tgcaatacct tcttcaatca attccttaag actttcttct gtttttccac
    23701 gttgtggttc ttttacaaac agcaagaaaa taattgccag taaaaaattg ggtattgcaa
    23761 ccagtataaa tggtaaccgc catccataga cagggccaat gaaccctgcc atgagctgcc
    23821 ccactgcaat tcctaaaccc tgtgcaagcc caatccatgc tgcagcagcc gccctgtttt
    23881 tatgtgaaaa ataatcccca atcagtgaaa atgttaatgg aagtgctccg ccaattccaa
    23941 ttcccgttaa tgctctgagc cagaataact gattataatt ctgtgcaaaa ccagtaagca
    24001 gacacggaat ttcaccaaca agaataacaa ataaaaatag attccgacgt gatattttat
    24061 cagtcagata accaatggca agagtcacaa gcccgcccag cacccagaat acaaacgata
    24121 ttcgtccgcc taacataaca tctcgctgaa catcattaaa accaaaatca tgggcaatct
    24181 gtgtcagatt gggtgccatc agattctgat cagcaaataa aaataaattc atcaatgcaa
    24241 tcaggagtat tgcaaatatt tcatgtttag caaatttttg ttcagccata cttattcccc
    24301 cacgatgtat atacttttga aatacgaaag tgcattgtaa ataccattaa tccataataa
    24361 attcaaggat tttttgttaa attatgtatc aacaaccttt ctgcattcag gagaaggcac
    24421 cagtaaaata ccgttttcag catcgccaaa tgaataacaa tttttacgcg aacaataata
    24481 taccgtatat gcgcacacta atgcatcaag ataatcttca tactttttta attgtttccc
    24541 tttagcctgt gcgatagttc ccgtaaataa atcactacac ttaattacag ctgctaaata
    24601 attatataat ttttcaatat tctctctgcg catagtaaca ccgtatttag atttatactg
    24661 taaaagtttc atgttgttaa aacatgtcat aattgttgca tgaggatata cttcaaaaat
    24721 attgttgtta ttaaaactga aaccaagagc atgcaattct ttgtataaca cttcaggaaa
    24781 aaagtatgga taccgttttt tattcactgc atgcagtcca agcccaaatc gcgaaaatac
    24841 tcttaaaaat tcaatttcat gcctgcgatg tcctgttgcg ttttttataa tcaaaggtgc
    24901 atcaatacct ataatagcgt cattaaaagg aactatcgcc gaaattattt ttttaatatc
    24961 atttgtataa caagtatcta taacctgcaa tgcatcatca cacattacta ctgctgtata
    25021 atttttacca ctccatgagg ggtctatgcc aatataatac atactataat cctgataaaa
    25081 agataagacg cactaggtgt gcgccttatc aaacgttttt ataaatgctt tatattttat
    25141 ccagcctgct ctttctgcat cttttcaagt tccatcttcc tgagttcttt acgcaatatc
    25201 ttcccaacat tactctttgg caaactgtcg ataaattcta ccatctttgg aactttgtat
    25261 gcagctaatt ttgttttgca atagtctata acttcctgct cggtcatggt ttcaccaggc
    25321 ttcaatacta caaacgcttt aacggtttca ccacgatatt tatcaggcac tcctattgca
    25381 catgcttcca taatttttgg atgttcaaag agcacttcat ctatatcacg tggatagatg
    25441 ttatacccac ctgcaataat catatccttc ttccgatcaa caatatagaa atagccattt
    25501 tcatccattt taccaatatc acctgtatat aaccatccat cgcgaagtgt ctttgctgtt
    25561 tcatcaggca tcttgtaata accactcata acctgaggtc cactgataca tacttcacct
    25621 tcttgcccca atggcatttc ttttgtacct tcttccagat caacaatctt tagctctgta
    25681 tcaggaactg gcaatccaat tgaaccctgt attgtttttc caccatacgg atttattgtt
    25741 gcaacaggcg aagtttcaga caatccataa ccctcacaaa tttgagcacc tgtttttgct
    25801 tcaaactctt taataacatc aacaggcaat ggcgctgcac ctgaaaaaca tcccttaata
    25861 cttgatatat ctgtgtcttt gatttttgga tgattcaaca atcctacata catagtggga
    25921 acagccggga agaaattcac tttatgtttt actatagctt caaggatggc accaggttct
    25981 ggtcgtggta tcaacacctg tgtccaaccc tgccagaggc aaaggttcat taccgtggtt
    26041 aatccaaatg aatggaaata cggtaaaatt cccaggcttg ttaactcacc gcgcttaaat
    26101 cccggaaacc atgcacttat ttgttgtaaa ttgcatgata aatttttatg cgtaagcatt
    26161 acacctttac ttacaccagt tgttccaccg gtatactgta aattggccag atcattgaat
    26221 tttgccattg tacccggatc tttatcaggg tatttcttta ataaatcggt ccattcatgt
    26281 acatcctgag ttggtgggat atctttatgt ttatcttttg ccacgattgg caaaagttgt
    26341 tttttgggaa atcctaaatg gtcacggata tgagcaatga taattttttt aaccggtgtt
    26401 tttgggcgta atgcaatcat gcgtggacct aacaaatcca gtgttattaa aacagttgat
    26461 tctgaattat ttagcttata ttccagttca tgatcagtat ataaaggatt attcattacc
    26521 acaacagcac caattttcca tgctgcatac gatccaatga ctatctgtgg catatttggc
    26581 attaacattg caaccttatc ccccggcttt acccccatat caagtaatgc atttgcaatt
    26641 ttgttaacca ttgactgcat tttcttaaaa ctgatcttag catcaagaaa gatcagcgca
    26701 tccttattgg gatattcttt tgctgacctt tcaaatccgt gcacaacggt tatctcatca
    26761 tatttgattg aatgaggtac acctggtgca taggatttta accagacttt ttccattgca
    26821 aacctcctgc gattttttta attttaaaaa aacagaaacg atagatcatt gaagtgaatt
    26881 tgtattttat gactttattc aaacaatata tgataacaat gtcaactatt tatttatata
    26941 taacatattt ataattttat tgaataatga taacatgata tacttttata tttataatta
    27001 tttttatatt cattatattt tttaatatag tatatatata aatacatatt tcaataacga
    27061 atatatttct actcaaaaga ataaggtttt attatttaca gtttagttaa tatttgtatt
    27121 ttttactatg cttaaaatac tatctattta aaaagaattt ataattagaa catataatta
    27181 tatacttttc gccatgttca ttgatatgtc cttcaaaagc aaaatataat tttaaagaat
    27241 attttttgtt ttaaaaatat ttctttaatt taaaaaatat tcattgcaaa ttaaattatg
    27301 tatacatcat tatcaatatt tgtattcaga ggtttattat gaaaaacaaa aaagtttttt
    27361 cacgaaccat tgatattacc tgttatgaag atgaacagga ttctgttatc atagaagggg
    27421 agatcaagga tacccgttta tgccattatt atctcgttac tggtgaaaga aaagaaccag
    27481 gcattctcca taatatgaag attactatga aggttagtgg aaattcattg actataagcg
    27541 atatccatgt tgaaatgata cattacccac gggaagaatg cattgaagca aataaaaata
    27601 tacagaaact gaatggtcta tccattacca agggatttac aaaagctgtg aaggatatta
    27661 ttggtggaac cagggggtgc acacacgttg caaattgtat cttagctatg gcaccagctg
    27721 ctgttcaggg tttctgggct cataaagcac aaaaacctgt tacgaaagaa tttgccaatc
    27781 agggatcaat gagccattat ttagttgata cctgttatgt atggcgcaaa gatggtccaa
    27841 tgataaaaaa attgtcagaa agtatcaata aaatacagag ctaacaaatg tattaagtat
    27901 aaattatatt ttcgaaggaa catgtaatgt ttggatatca tggcaaaata gttgaagtta
    27961 acttaactac taccagcata aatgttaaaa attttgacgg agatttcgca aggctttatt
    28021 taggtggcaa cggttttgct gcaaaaatac tttatgacaa cgtaaaacca gacacagacc
    28081 ctctatctga agataatgtt gtagtctttg caaccggacc ctttac
//
LOCUS       DAORYL010000036        27997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1459_length_27997_cov_13.297795, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000036 DAORYL010000000
VERSION     DAORYL010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 27997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27997
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1459_length_27997_cov_13.297795"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1822)
                     /locus_tag="PLV81_08090"
     CDS             complement(2..1822)
                     /locus_tag="PLV81_08090"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOM10099.1"
                     /translation="MKLTDLKHKALKKWVEDAIALMQPEDVYVCDGSKAEYDRLAEQM
                     VAEGLAIRLNQKKRPGCLLFRSDPSDVARVENRTYISSKTKEAAGPTNNWIDPVELKQ
                     TMTDLYRGCMRGRTMFVIPFSMGPVGSPIAKIGVEITDSPYVVINMHIMTRVGAKVLD
                     VLGTDGEFVPCFHSVGKPLAKGESDHGKWPCAPLEKKYISHFPETREIWSYGSGYGGN
                     ALLGKKCLALRIASVMARDEGWLAEHMLILKITNPEGKTKYIAGAFPSACGKTNLAML
                     VPTIPGWKVETVGDDIAWLKFGSDGRLYAINPEAGFFGVAPGTSEQSNYNAMKTIEKN
                     TIFTNVALTKDLDVWWEGIGYEPDGKLIDWTGKEWIYDKNNKDQAPAAHPNSRFTTPA
                     SQCPSIAKEWEDPDGVPISAFLFGGRRAHTIPLVLESKNWLHGVFLGSIVGSEVTAAA
                     LDLKVGQVRRDPFAMLPFCGYHMGDYFKHWIQIAQKTTPDKLPKIYFVNWFRKTDDGK
                     WLWPGYGENSRVLKWIFERCDGSGKAIETPIGNMPTIDAIDRPSSVPVQDMEVLLEVD
                     TEGWLQEVDDIRTNHYPKFGNHLPKELYALLDEMEKGLRK"
     gene            complement(2376..3293)
                     /locus_tag="PLV81_08095"
     CDS             complement(2376..3293)
                     /locus_tag="PLV81_08095"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOM10100.1"
                     /translation="MIHINNTSTYHKEQITTLGWELTVCNMLYPENSPCREVIKKNAP
                     YGTLLCEFLSSFIDMKQVKTILEAGGGYGFLMKDFLACCPHVQAIMVDISPAMLAEQK
                     KNIRGNVSFIHSDFLELDNALLSDIDLIICNEIIGDFITVCDITHDILESFTLDEIKE
                     CKRLINEYNLEIEANPFNFNLGALLAIEKICKAGTKYIYISEHSCESESQGEFRECVP
                     VRPTFNPYEIRLFGHSEYTIRFSHLEAVAKKWGYTIHRGVYNDIFGCRMTPKLQFILN
                     STASAKDEYEILRQFIGDMFTYEYIVGCK"
     gene            complement(3290..3670)
                     /locus_tag="PLV81_08100"
     CDS             complement(3290..3670)
                     /locus_tag="PLV81_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10101.1"
                     /translation="MHIIKPCPACGIKLRFPIDSGKIKVQCRCGYSFVADPDDPVLYN
                     DATFDLALKKKPKTRLSYTSIIKTIIEKLYSYWYTLGNFRLLPTKDKIKVIAIALVII
                     LLLLLFVYYIFFWHSQPPENGIVI"
     gene            complement(3680..4540)
                     /locus_tag="PLV81_08105"
     CDS             complement(3680..4540)
                     /locus_tag="PLV81_08105"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOM10102.1"
                     /translation="MHEHIQQRIQSIIQNVDQLPSLPDVVSRIINMVNDPNVSFKQVA
                     EEIAKDQAITANILKLCNSAYFSKGKEISSIDRAIVILGLKEVKDIVVLATTKSVLNR
                     VILGYDLARGELWKHGVAVAMLAKKIANDCNQKTVADIAFTGGIIHDVGKTVLALFVQ
                     STFKDILSTVTEKNITFQEAEKLIMGFDHQQIGQQVTIKWKFPKVLQSIVRYHHEPTN
                     APDEHKKIVSIVHIANTLCLMAGIGIGSDGLYHELNQDAVTMLGLQDAQLEKFYSDIP
                     ELMNKARDIL"
     gene            complement(4564..5043)
                     /locus_tag="PLV81_08110"
     CDS             complement(4564..5043)
                     /locus_tag="PLV81_08110"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOM10103.1"
                     /translation="MDNLINIGVAQLAVAENPAVLRTILGSCVGICIFERNKKIGGLA
                     HILLPKNPGDSNPEKYADTAIPILVQQLLKKGCKKEFMSAKIAGGASMFKFKSTTTLA
                     NIGENNIEATRQALAKFGIPIVAEDIGGSAGRVIDFFLDDGRLKVKASGQEKMYYKV"
     gene            5173..6090
                     /locus_tag="PLV81_08115"
     CDS             5173..6090
                     /locus_tag="PLV81_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10104.1"
                     /translation="MALNQINNYIRLIDAQNVNKTGNIYINILKNEFIMLNEEISIPK
                     IPVSKLSYIEALPIAQTIIPLIPLFLFGHTLLEERQPAHELHSLHFIRLLEGRCINFY
                     HVLRVDFKFGGDSSAILEPGNNDYYPSYRTNRLYYKSRLVPTFKDPSPPITPIKLIQS
                     ITTESDQYFHTYAMFDDIDTSNITNEFIKTLPDIFSIPSNLYSFIVMDYYTACMNIPN
                     PIPLELDRAVIIFEPLFFIIASHFIPIDSIISLHELEAHFPELIAIKDQKLVPTPNLI
                     QMAKEYFNRYSLTRDEQCMLKGWWQLVIA"
     gene            6083..6592
                     /locus_tag="PLV81_08120"
     CDS             6083..6592
                     /locus_tag="PLV81_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10105.1"
                     /translation="MRNIIYLLPGIFLFITCANKPLILIRAECGSSVSMNGTIANDSL
                     TYTFKPCTHCRSLKDVSNYIYFRGDTLCFSIQCNQNIESATVTAYFENPTTHTTYKAE
                     RLEKVTTAVYGFSLIGSLLETIYWDDIRNTHSIKTLKLPFVIHVTIQKQNQKVHHTFS
                     DTLYVQFVD"
     gene            6664..7518
                     /locus_tag="PLV81_08125"
     CDS             6664..7518
                     /locus_tag="PLV81_08125"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOM10106.1"
                     /translation="MKKNKSNMYVRFWGVRGSIPTPGKDFVTYGGNTSCVELRCGQTI
                     LIFDAGTGIRMLGKALSKEFSGKPVTVHLFISHTHWDHIQGFPFFDLAYNKHASIYFY
                     GGHSVSTLEKLILGQMDREYFPVTLFELSSNIHFVELSHNPFTINDITIWYTHLFHPG
                     LSLGFRVEYNNHVFVYASDNEVLNDPELGDYNLKNIGNLIQDADILVADCQYTPQEYA
                     TRIGWGHSSIESVAGVASRFNVKHLITFHHDPNHTDRDITRAVKKARSLSLPIRITAA
                     KEGDILYI"
     gene            7562..8176
                     /locus_tag="PLV81_08130"
     CDS             7562..8176
                     /locus_tag="PLV81_08130"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOM10107.1"
                     /translation="MKSIVLHNTYLCMRHAQSTANEKHIIISDPTTGISKYGLTKEGE
                     RQVLEAIVTNKEMRNISYIYTSDFLRAYQTATIVSRLCNNVPIITDARLRERYFGLFE
                     GTNSENYHKVWDMDCTSEFDVACYNVEPIHSVARRMLSFLLDSEIHPTNQKILVVSHG
                     DPLQILYAVTRGLAAHQFKSLPHFANAEIRILPSHFTIPEEYLS"
     gene            8173..8373
                     /locus_tag="PLV81_08135"
     CDS             8173..8373
                     /locus_tag="PLV81_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10108.1"
                     /translation="MNSPWLPFIYQYSIFTIFFIFTTMIAIKKHALSLSHPTERRILF
                     EIIAGYLFFIVFHASMIIVAGV"
     gene            8377..10191
                     /locus_tag="PLV81_08140"
     CDS             8377..10191
                     /locus_tag="PLV81_08140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOM10109.1"
                     /translation="MIKGTVLDFAIIVCYFLFILGFGTFFGKYVKSTKDFFFGGQRFS
                     WWLIGFSCVATTVGSYSFIKYSAVAYQYGFSSTMTYLNDWFILPLFVFIWLPIIYYSR
                     ITSIPEYFEKRFNSTTRTISVFILLVYLLGYIGINLYTIGVAFNAMFGWDVMITAILI
                     SFVCAVYMHAGGQTSVIMTDLAQALMLLSAGFLVFFLGIEKIGSVSAFWKSLPYEFKL
                     PFSPFNKPPDFNFVGIFWQDAFGSSMAFYFMNQGILMRFLSVRSPRDGRKAMFLVCLV
                     LMPLAAFAVANAGWIGRSMQNLGLLSNSTDPNHIFVDVAFGITKPGIFGFIMAALIAA
                     LMSTIDTLINGVAAIGVNDIVKKFVPDREDTFYLKWARTISLAAAVIGVALVPLFMQF
                     KSIYLAHGTFIAAVTPPMIVTILFGILWKRYTTKAAIYTLVIGSIAMLISIQWPSVLT
                     LLSHGVAPEGLQYLRALYGLIVCTAVSIVITVLSKEKELSNIPGLVVDTIHEGKVLFK
                     GGEPDDFDEGKIIHCYIAETSEGVSLSTKMMEYLQAKPGDILYIADERWWLGGLRSIH
                     AKAGTPHQGVDNVLFLGSDLITQGNLDINRKVTVIKII"
     gene            complement(10199..10777)
                     /locus_tag="PLV81_08145"
     CDS             complement(10199..10777)
                     /locus_tag="PLV81_08145"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HOM10110.1"
                     /translation="MTDIVNTFISNYGTLFLIFVARIFDVSIGTMRIILMARGYRSIA
                     PVLGFFEVLIWLVAINSALKHFDGAISYIVYAAGFATGNYVGMLLEEKIAIGYQSVRI
                     ITSKVVSALPLVLRQEGFGVTQIDGIGMKGPITLLFAVVPKKDVKRVVEIVHLLEPNA
                     FITIEDVKQHLSGFSVHNGFAQKFAAFITKKK"
     gene            11018..12439
                     /locus_tag="PLV81_08150"
     CDS             11018..12439
                     /locus_tag="PLV81_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOM10111.1"
                     /translation="MSGYLIGILAYIIFQLILGIIVSRKIHSDDDFILAGRTLGYLLV
                     TFSVFATWFGAESCIGTSGAAYQDGLVGVTADPFGYAIVLFVLGLFFASRLWNMKLTT
                     ISDFFRNTYDSTVEKLTAIILIPTSLLWAAAQIRAFGQVLSASSELELTISITISAIV
                     VIVYTGLGGLLADAITDIVQGAILIIGLFVIFFSVLGDVGGLSQAIGMIDTTRLTFVP
                     HSITNPWQRLLTMAESWAVPICGSLVAQEVISRMLAARSATVARRSSLLASLMYISIG
                     MIPLFIGLVGYHVMPGLNDPEQILPKMAQFHFHKYLYIIFAGALVSAILSTVDSALLA
                     SSALLNHNLIFNFYPNLSDRAKLIADRTGVLLLGITSYILALHAEGIYALVKEASAFG
                     SAGVFVIYIIGMYSQKASVPAAIVTLITGATTYFIGKFFIHFEYSFLLSVLIAVLSYY
                     AVMLVTRVISSAELPVAVKDTDS"
     gene            12531..12818
                     /locus_tag="PLV81_08155"
     CDS             12531..12818
                     /locus_tag="PLV81_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10112.1"
                     /translation="MKRKYGIIVLILFVIIRPLHATVFSHGAYSSHTSFHLVTDDSTT
                     NKEQPPSKNNQDEHPQDDSSNDDSMDHNDSESDEDLEDIPDDFYDYQEENF"
     gene            complement(12815..13261)
                     /locus_tag="PLV81_08160"
     CDS             complement(12815..13261)
                     /locus_tag="PLV81_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOM10113.1"
                     /translation="MFKKILYPTDFSECAHASIPYIKLMKKAGTKELIILHVIDIRQS
                     TIIDTTAFGETFVYPYDISDILKKEAETQMNTLIKKFSRLFKVTPLIVEGIPFKEIIH
                     AAQQYNVSCIVMGSHGKSNIEEMLLGSVTEKVVRKSPIPCLVIKRA"
     gene            13314..14060
                     /locus_tag="PLV81_08165"
     CDS             13314..14060
                     /locus_tag="PLV81_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10114.1"
                     /translation="MSDSNQEKKSPFDDYNFAVMYEKRNAQFGYPGKLLDHIMKYLKQ
                     YNVTSVADVGAGTGAFSIPLAIAGFTVYAIEPAAGMRSILDDKIKNLSVNVTIYPHTL
                     ETIKELKADSCIAMHSLYGIKPLEKALVAMMHIAPLVLIAVRTEKTYTLSDVIRSYFK
                     KERSKHHLQHIIDYLEKHSISHEIHSIHQAHKVLIHDIYQEALFHCYAMGFDESYVST
                     IMTILEKHLHNNGSYWFENVHDDAIVIIHA"
     gene            complement(14062..15033)
                     /locus_tag="PLV81_08170"
     CDS             complement(14062..15033)
                     /locus_tag="PLV81_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10115.1"
                     /translation="MLLITVSCSREKEINEGIRLYNEGLTQSAFVKFREGLLHYSTIK
                     TINAQNLYFSPCAIFSSENILSMLYPEQYSYNFPREVVSVWYDREEDTCVVTDGIVVY
                     LSIQDKESIINNPSQEPIKAVAHATHTYFLSGKELFEYNPKIAQIKKLSSVEFSPPEA
                     FKYYNAYIDIVDDKLIVIAGIAGAYNLWVITKNGEVLISSLKVASWKHAVYDGTLFII
                     KGSAGNWDVKSINIKTGQEKIIKNFSSLTDIHMTDHGIVINTGEKMYIYLFNDTMYVL
                     PFVYSIKGETRDGMVVDNGSLLIISSEKFFSKIQRIAKDTGCCTVKN"
     gene            complement(15066..16421)
                     /locus_tag="PLV81_08175"
     CDS             complement(15066..16421)
                     /locus_tag="PLV81_08175"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM10116.1"
                     /translation="MSLLSITFFIVGASIVGALLLVIRAHQYPAKVRKAQEYLDSGDI
                     AKASEIVKLVLERKKDYVPARYLRAQILVKQKQYLLAISELNGILALPEFNKHVNELD
                     IHYMLADLYHETQQWQKEVEEFRIILQFNPDDINANHRLGIVLYRQHDYIGAKDYLLK
                     AVTMDPALTDIYAPLGICLFNLADYELAQNFLIKAIESNPSNYEPYYYLGMIYKMKKD
                     YVAALDMLSRARNDRRYFVKSQYAIGQIYFDNDEYSKAIEELEKGLSYVKDSTDEGLA
                     YRYLLAECYEMENKIQEAVHHWEKIASINPNYRSTQLKLDDYKAILENKNLKFLFTSS
                     MSELQPLITEIIAQLNYSIVYKKEVAPNEYMYKCFSVKRINEPPALINFIRTTRDITE
                     GQINAFYQRMLEEKCRSGIFITTSKFSINAKSQAANKMIEIMDGRFLQKTMEKIKGKK
                     V"
     gene            16534..17064
                     /gene="purE"
                     /locus_tag="PLV81_08180"
     CDS             16534..17064
                     /gene="purE"
                     /locus_tag="PLV81_08180"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOM10117.1"
                     /translation="MAEVGIIVGSDSDLPKLKGCFQTLDDFGISYDVKAYSAHRTPHL
                     VAEWIKLMESAGAKVFIAAAGGAAHLPGVVASHTVLPVIGIPIETQLSGGLDSLLSIV
                     QMPSGIPVATVATGKAGATNAALLAVSILATANAAYQEKLRNYRNTMQKTIIEKNETL
                     SKQGLYEYIKSLEAKK"
     gene            17061..18629
                     /gene="guaA"
                     /locus_tag="PLV81_08185"
     CDS             17061..18629
                     /gene="guaA"
                     /locus_tag="PLV81_08185"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOM10118.1"
                     /translation="MIEHGIGVASQNKVLILDFGSQYTQLIARKIRELHVYAEIVPYT
                     IPIDEIKKENPKALVLSGGPSSLYEEDSPKIRKELFSLGIPVLGICYGLYVVVDAFGG
                     VISPSPFKEYGRADITIVKDSPLFKGLPDTQTVWMSHGDKVESVPQDFTIIAGSRNAK
                     IAAIEYRQKKIYGVQFHPEVYHTQYGKEILHNFLFEICDFKPTWTMQSFIDSAINDIR
                     TVVGNGTVLLGLSGGVDSSVTAVLLQKAIGNRLYCVFVNNGLLRKDEHIKVIERFKKH
                     LNLNLIYADAEELFLQKLKGVTDPEQKRKIIGKEFINVFKAEAAKIGTFDFLAQGTLY
                     PDVIESVSTKGPSDTIKSHHNRVPEVLELIKEGKVIEPLKELFKDEVRELGRQLGMPE
                     ELINRHPFPGPGLAIRIIGEVTKERLFILREADDILIEEIKKADYYTKLWQAFAVFLP
                     IKSVGVMGDKRTYENVIAIRAVTSLDAMTADWAYLPEDLMRRISTRIINEVKGVNRVV
                     YDISSKPPSTIEWE"
     gene            18793..19083
                     /locus_tag="PLV81_08190"
     CDS             18793..19083
                     /locus_tag="PLV81_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10119.1"
                     /translation="MLLRDYDAEQIKDEITQCVATNVFDSEPESYTKAKELLQELFIV
                     SGGNFNRPDYESIMKVLKLIAARQLKKGKDTKAIAERFKEIAKKIESVAVYT"
     gene            complement(19084..19536)
                     /gene="smpB"
                     /locus_tag="PLV81_08195"
     CDS             complement(19084..19536)
                     /gene="smpB"
                     /locus_tag="PLV81_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOM10120.1"
                     /translation="MKEEKEIIQNKKARFEYEILDTIEAGIVLKGTEVKSIRQKKVSI
                     QDSYARIKDGEAFLYGMNISPYEAGNIFNHDPLRVRKLLLHKQEIKRLTGKQQEKGLT
                     LVPLKLYWKNGKVKVLLGLAKGKTLYDKRHDIKKRDADRELQRYKRMR"
     gene            complement(19575..21734)
                     /gene="ccsA"
                     /locus_tag="PLV81_08200"
     CDS             complement(19575..21734)
                     /gene="ccsA"
                     /locus_tag="PLV81_08200"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOM10121.1"
                     /translation="MNLLYYLPWLSFVLVVASVILAVKSFKKENLLKAAHYSYYGATV
                     VIGFMLVALTIAFVLNTFQFLYVYQHSSVDLPLLYRIAAVWAGQEGSFLLWTFFVMVT
                     VVLALRYEKHHVQSFLTLAGLVTLVFIIHCLHNNPFMYVWDASKNVAPGVIPHDGMGL
                     NPLLQDFWMVIHPPVLFAGYALATVPFVYAVNALLYNDYSVMTTGYRWVVATVLTLGA
                     GIFLGGYWAYKVLGWGGYWGWDPVENASLIPWLTGLALMHGVMVQKRKKALIRFNIIM
                     AIVTFVLVIFGTFLTRSGVLSDFSVHSFSGAGNFTDLLLFIVTIGIAATGLLWYRRND
                     AIGKTLDNTLFAKDNIMVYGIAALLFFALFVLIGTCMPIITGLLGQASTVQESYYVSI
                     ARPIGFLIVVLLLLTPFARKPTLKTVLIAGTIALTVSLLLFVGKPFVPVAFILMVLSF
                     GILLTNVKGIVPHIPLSLAARIAHSGVALLIVGIITSQTQSVEYHAQIAQNEKKTVYP
                     VTLELVGMVEGIKSAIVFKYDYYNTSKTIQTPYFIDTRTNQLFREPYIDYNIGYDVYI
                     SPVEYRLGKDTLTQIMLKKGEVRSFNDVTVRFDGFDIDRMAMMQGNPKIYALCTIKYQ
                     GKEYTVRPGVLFDGDRRTNVNSMLPGTTRTISLMDFDIHEKLIVLYMDAPANTTVPPD
                     FVIVDISFKRLIWLVWLGTVVIALGTALPLVKRPWNT"
     gene            complement(21734..22105)
                     /locus_tag="PLV81_08205"
     CDS             complement(21734..22105)
                     /locus_tag="PLV81_08205"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HOM10122.1"
                     /translation="MKKSNIIIVSIIVVCGAVALFSFKGILTPYVSFDEAAKGEAYVQ
                     VLGKLVKSVPVQNSADGFIFTIEDDNAKRLTVIHKGSKPLNFEHATSVVAIGTYNKEK
                     QIFEADKILVKCPSKYTKENK"
     gene            complement(22092..22265)
                     /locus_tag="PLV81_08210"
     CDS             complement(22092..22265)
                     /locus_tag="PLV81_08210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HOM10123.1"
                     /translation="MKLLFMVTIAQNITQIEKSPTTVLFWVSAVVWIGIVCYLLLLHN
                     KVKKLEKRLNEKK"
     gene            complement(22262..22915)
                     /gene="ccsA"
                     /locus_tag="PLV81_08215"
     CDS             complement(22262..22915)
                     /gene="ccsA"
                     /locus_tag="PLV81_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOM10124.1"
                     /translation="MVLIIIDALLMLATLYLIYIWVPPAAILGETSRIVYLHVPLAWV
                     STLAFIVAGLASIQYIRKNAIEYIHCARSSVHIGFFFSILTVITGSLWAKVMWGSYWN
                     WDPRETSITLLLLVYIAYFSLHQIVHTNPKGAMLESVYLVIAFVASPFLIFVVPRLYP
                     SLHPEPVINTQGAILLDTEMRLVLIISVVTFTLFYAIVLKLLLKVYKLEYVCTEDVS"
     gene            complement(22945..23604)
                     /locus_tag="PLV81_08220"
     CDS             complement(22945..23604)
                     /locus_tag="PLV81_08220"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HOM10125.1"
                     /translation="MIKGLVRNIVKDLRIEFRSKVVISLILSFSVIVTISIGFASGGI
                     IGSPMVHAILLWVIIFFSGMNTSHLFYREIEEHTALFNLMYYSPEGIFVSKLIVNCII
                     MVAVAAIAGLLYLFVMDVSVYNVSGFAIMVLAGSSALAVCTTIIAAITALVQARGGLF
                     TILSFPITLPIITVVIKSTTQCFSKEIYSSWSAILFLLAFSTLLGVVSYILFPYIWRE
                     F"
     gene            complement(23582..24193)
                     /locus_tag="PLV81_08225"
     CDS             complement(23582..24193)
                     /locus_tag="PLV81_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOM10126.1"
                     /translation="MFTIKAHNISKRYSYRLFSNISCSVSTGESVAIVGPNGSGKSTL
                     LKILGNIVSPDSGRVEYFDKMESIPAHTVIHYAGIVAPYIQCYNHLTLTENIIFAFGS
                     VTDDAIELLHRLDMYQHKDKVLHTYSTGMQQRCKIAMALLKKPKVLLLDEPGSNLDDS
                     GRKILFELIKEIQPFTAIIMATNDSREAKLCHRSIVLDKGIGA"
     gene            complement(24200..25558)
                     /locus_tag="PLV81_08230"
     CDS             complement(24200..25558)
                     /locus_tag="PLV81_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HOM10127.1"
                     /translation="MKSKIVIIISMVALTVYAFFTGCKSTPHYTLAQFISPATCGGCH
                     DEIYEQWKNSMHNLAQKDIIYKEYAAFYLDDIKKQNPVNKDELEEAESCVKCHIPVGF
                     ITGKPVTVTEELAMENELPEITKEGIQCDYCHVIIDAKKNYNAHFTYKPGNGEADPGV
                     KYGPFKDATPDWHQAAYSKFHTQAAFCGTCHDVRHVVYNTKLETTFEEWSKSPYNTGD
                     EKTTVPCQGCHMYHRPGVPATGSTPRPNNPGFAAFGGPQRDHIFTHYFVGGNSIIPGL
                     YNDKVKGKMAEERLKNAATILIDTRDISTGAFAVTITNTGAGHKLPTGLTDVRQMWLE
                     IVILNQYGKEVFSIGKADKRGYLPDNTIIFKTIFGDGKGKPVANIAKAKEILFDNRIS
                     PKESATNTIILPKGLKGQYSIQVRLLYRLADQKTVDEVMKDKAVKLPVIEMATAKKEI
                     VL"
     gene            complement(25734..26765)
                     /locus_tag="PLV81_08235"
     CDS             complement(25734..26765)
                     /locus_tag="PLV81_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOM10128.1"
                     /translation="MDLLQEYFFIFIPVVYFGVAFTVLLLFKKIVFSLLTKWAAATSW
                     DIDDIIIDGLKKPAFFVVLALAILIASQYTILSEKWQMLISKSVHVIIIFALTLGIAN
                     IVGSLLQKYVKIANIPLAPTGLTYIIIKGLFVLIGILIILNYLGISIAPILTTLGVGG
                     LAVALALQDTLSNLFAGMQILIERSVRVGDFVKIDEGIEGYVEDITWRTTRIRMLPNN
                     IVIVPNSKMAQSMITNFYLPVPHLALRIPVSVSYNSDPQHVEHVILDEVKKSASTIQQ
                     LVMDPEPVVRFIPGFGDSSLDFTLIVYVKEYADQFPVQSELRKRIFNRLKEEGIEIPF
                     PQRVVHIVK"
     gene            26958..>27997
                     /locus_tag="PLV81_08240"
     CDS             26958..>27997
                     /locus_tag="PLV81_08240"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HOM10129.1"
                     /translation="MKKILKWVAIVIGSVFALIIIASIVLMLVVTKDMIAQQMEKALN
                     RHVTIEKIDVSIFSVVSGIEVKGVAISNFKTPKQLEALKDKPVDKGDLFVGLDSFTFK
                     LKFLPLLQGKFELRELLLTGPKINITRYQSGAFNFSDLMKPSKEEKKVEEVKKEEPSK
                     PLTADTLPISITIGKVGIERGTITFVDQSLQQTFQLYNLNALVHDIEIDPKDLAKKNQ
                     VKLKLDIGIKTIGKTSGGSVESFDIGLASRGTVKPFDLKTRILNPEIALKAGSPYGTM
                     TGLQIFDAIKSNETLANYAGKFDFLSKDVKWKNGYVDIWYKGGLLKITNGKISTDDYA
                     GNFKADVNFNTKA"
BASE COUNT         8635 a         5596 c         4946 g         8820 t
ORIGIN      
        1 gttatttacg taaacctttt tccatttcat caagcaacgc atacaattct tttggaagat
       61 gattaccaaa ttttggataa tggttggtac ggatatcatc aacttcctgt agccagcctt
      121 cggtgtcaac ttccagcaat acttccatat cctgaacagg tacgcttgat ggcctgtcaa
      181 ttgcatctat tgtgggcata ttacctattg gtgtttcaat tgcttttcca cttccatcac
      241 atcgttcaaa tatccatttt aatactctgc tgttttcacc atagccgggc catagccatt
      301 taccatcatc tgtttttctg aaccagttga caaaatatat ttttggcaac ttatcgggcg
      361 ttgttttttg tgcaatctgt atccagtgtt taaaataatc acccatgtga taaccacaga
      421 acggaagcat agcaaaagga tctcttcgga cctgccccac cttaaggtcc agtgcagcag
      481 cagttacctc tgaaccaact atcgaaccca aaaatacacc atgcagccag tttttggatt
      541 caagtaccaa aggtattgta tgagcacgtc tgccaccaaa taaaaatgca ctgataggaa
      601 ctccatcagg gtcttcccat tcttttgcaa tagatgggca ctggctggcg ggtgtagtaa
      661 agcgtgaatt gggatgtgcg gcaggtgcct ggtccttatt atttttatca taaatccatt
      721 ctttacctgt ccagtctata agcttcccat caggttcata accaatacct tcccaccata
      781 catccaagtc ttttgtaagt gcaacattgg taaatatggt attcttttct atagttttca
      841 tagcattgta atttgattgc tcagatgttc caggggcaac gccaaaaaaa cctgcttcgg
      901 ggttaattgc gtataagcgc ccatcacttc caaattttaa ccaggcgata tcatcaccaa
      961 ctgtttcaac cttccatccc gggattgtag ggacaagcat ggctaaattt gttttcccac
     1021 aggctgatgg gaatgcacct gctatatatt ttgttttacc ttccgggttg gttatcttca
     1081 gtatcaacat atgttcggca agccatcctt catcgcgcgc cataactgat gcaattctga
     1141 gtgctaaaca ctttttccct aaaagggcat taccgccata gcctgaacca tacgaccata
     1201 tttcacgagt ttcaggaaaa tgagaaatat attttttttc aagtggggca catggccatt
     1261 tgccatggtc actttcacct tttgctaaag gtttacccac tgagtgaaag cagggaacaa
     1321 attcaccatc agttcccaat acatcaagca cctttgcccc cacgcgggtc ataatatgca
     1381 tattgataac aacatacggt gaatcagtaa tttccactcc aatttttgca ataggtgaac
     1441 caacggggcc cattgagaaa ggaataacaa acatggtacg tccacgcata caaccacggt
     1501 aaagatcggt catggtttgt tttaactcca caggatcgat ccagttgtta gtcggaccag
     1561 ctgcttcctt cgtttttgaa gaaatatacg tcctgttttc aacacgtgca acatcacttg
     1621 ggtctgagcg gaacaaaagg caaccaggtc gtttcttctg gtttaaacga atagccagcc
     1681 cttcagcaac catttgctct gccagccggt catattcagc tttcgaacca tcgcatacat
     1741 agacatcctc cggctgcatt aatgcaattg catcctcaac ccatttttta agtgctttgt
     1801 gcttcagatc tgttaatttc atagcttatc ctttctattt cagtatacat tggggattat
     1861 aaataaactg acgttactgt ggtatggtat gacaattttg tcaacaattc tgttaagata
     1921 catatatagc aaaaaatatt ttatttttca aatattaact gatgcaggat atgattgaca
     1981 agaaaggttt tgtgtatcac tgtatttgta aaatagtatc aacccgtaga gaacgaaaca
     2041 tcctcagaat atccccaggg atattctgta tttcacattt tttaccattc tgcatactag
     2101 ttttttttat atcaattaaa aaggcaatac cagccgagtc aatatagttc aatcctgaaa
     2161 gatcaattgt taaatcatca ggagcggata agtatgcctt ttttacatct tcctttaaat
     2221 tataggaaga atataaatca agatcccctt caattttcag gatataaccc tgttctgttt
     2281 tattaaatga atataccatg cttgttaaaa gtaattaccg tactgttctg aatttcggac
     2341 atatgtggca gcttattgag taaatttttc accatttatt tacagcctac aatgtattca
     2401 taggtaaaca tatcaccaat aaactgccgt aatatttcat actcatcctt tgcactggca
     2461 gtgctgttta aaataaactg taattttggt gtcatacggc aaccaaatat atcattatat
     2521 acacctctgt gaatggtgta cccccatttt tttgcaacag cctcaagatg cgaaaagcgt
     2581 atggtatatt cgctatgtcc aaacagccgt atttcatatg gattaaaggt tggccttaca
     2641 ggcacacatt ccctgaattc gccctgtgat tctgattcac agctatgctc acttatatat
     2701 atgtattttg ttcctgcttt gcatattttt tctatcgcaa gaagtgcccc taaattgaaa
     2761 ttaaatggat tggcttcaat ttcaaggtta tactcattaa taaggcgttt gcattctttt
     2821 atctcatcta atgtaaaaga ttccagtatg tcatgcgtta tatcacacac tgtaataaaa
     2881 tcccctatta tctcattaca tataataaga tcaatatcag atagtaaggc attatccagt
     2941 tccagaaaat ctgaatgaat aaatgaaaca ttaccccgta tgtttttctt ttgttctgca
     3001 agcattgcag gagatatatc aaccatgata gcttgtacat ggggacagca agctaaaaaa
     3061 tccttcatta aaaagccata tcccccacca gcttccagta ttgttttaac ctgtttcata
     3121 tcaataaaac tagaaagaaa ttcacacaac agcgttccat agggagcatt tttcttaatt
     3181 acttccctgc aggggctgtt ttctggatag agcatattac aaaccgtaag ctcccacccc
     3241 agtgtggtta tttgctcttt atgataggtg ctcgtgttat ttatgtgaat catatcacaa
     3301 taccattttc tggaggttgt gaatgccaga agaatatata ataaacaaaa agcagtaaaa
     3361 gcagtataat aactaaagca atagcaataa cctttatttt atcttttgtt ggcaataatc
     3421 taaaattacc aagtgtatac cagtatgaat ataatttttc aattattgtt ttgataatac
     3481 ttgtataaga caatctggtt ttgggctttt ttttcaaagc taaatcaaac gttgcatcgt
     3541 tatacagtac cggatcatca ggatctgcta caaaggaata tccacaacga cattgtactt
     3601 ttattttacc agaatcaata ggaaaacgca gcttaatgcc gcaggcagga caaggtttta
     3661 taatatgcat acgtaaatcc taaagaatat cccttgcttt gttcatcaat tctgggatat
     3721 cactgtagaa tttttccagt tgtgcgtcct gcagtccaag cattgtaact gcatcctgat
     3781 tcaattcatg atataaccca tcgctgccaa taccaatgcc cgccatgaga cacagagtat
     3841 ttgctatatg gactattgat actatttttt tgtgttcatc aggagcattt gtgggctcgt
     3901 gatgatagcg aacaattgat tgtagcactt tggggaattt ccactttatg gtaacctgct
     3961 ggccaatctg ctgatggtca aatcccataa tcaatttttc tgcttcctgg aatgtgatat
     4021 ttttttcggt tacagtggaa agaatatcct taaatgtact ctggacaaat aatgccagaa
     4081 ccgttttacc tacatcatgt attatcccac ccgtaaaagc aatatcagcc actgttttct
     4141 ggttacaatc gtttgcaatc ttttttgcaa gcattgcaac tgcaacacca tgtttccaca
     4201 actcaccccg tgccaggtca taacctaaaa taaccctgtt taataccgat tttgttgtgg
     4261 caagaacaac aatatctttt acttctttaa gccccaggat gacaatagca cggtcaatag
     4321 aagatatttc cttacctttg ctgaaataag ccgaattgca caattttaaa atattggcgg
     4381 tgattgcctg atcttttgct atttcttcgg caacctgctt aaatgagaca ttagggtcat
     4441 tgaccatatt gattatcctg cttaccacat ctggcaaaga aggtaactgg tcaacattct
     4501 gaattatgga ctgaatcctc tgttgtatat gttcatgcat aattattatc tccaatccat
     4561 ctatcaaacc ttataataca ttttttcctg gccactggct tttactttta accttccatc
     4621 atccaggaaa aagtcaataa ccctgccagc actgccacca atatcttcag ctacaatagg
     4681 aataccaaat tttgccagtg cctgccgtgt tgcctcaata ttgttttcac caatatttgc
     4741 cagcgtcgtt gttgatttaa atttaaacat tgaagcacca cctgcaattt ttgcagacat
     4801 aaattccttt ttgcaccctt tttttaataa ctgctgtacc agaataggga ttgctgtatc
     4861 tgcatatttt tcaggattgc tatcacccgg atttttgggt aataatatat gagcaagccc
     4921 ccctatcttt ttattccgct caaaaataca tataccaaca cagctaccca gaatggtacg
     4981 caacactgca gggttttcag caactgcaag ctgtgctaca ccaatgttga taagattatc
     5041 cattctgtct accaattata aaaaattgcc atttccatta aatattatct tgataaaaaa
     5101 ttacttcatt atgtatattt gtcaaggcaa aaggcaatat ttattacaat aaatttacca
     5161 ggagaagtgg gaatggcatt aaaccagatt aacaattata tccgccttat agatgctcaa
     5221 aatgttaata agaccggaaa catatatata aatattttaa aaaacgaatt tatcatgctc
     5281 aatgaagaaa taagcatccc taaaatcccg gtatcaaaat tatcatatat cgaagcgtta
     5341 cctatagcac aaactatcat ccctcttatt ccgctttttt tatttggtca taccttactc
     5401 gaagagcgcc agccagcaca tgaactgcat agtcttcatt ttattagact gcttgaaggc
     5461 aggtgtatta atttttatca tgtattgcgg gtggatttta aatttggtgg cgatagctct
     5521 gcaatacttg aaccaggcaa taatgactac tatccatcat acagaaccaa caggctttat
     5581 tataaatcac gacttgtgcc tacatttaaa gatccttcac cgccaataac accaataaag
     5641 cttattcaat ctatcactac cgaatcagac cagtattttc acacgtatgc aatgtttgat
     5701 gatatcgaca catcaaatat aacgaacgaa tttataaaaa cattacccga tattttttca
     5761 ataccatcaa atctttactc ttttattgtc atggattact atactgcatg catgaatatt
     5821 cccaacccca tccctcttga acttgatcgt gcagttataa tttttgaacc actttttttt
     5881 atcatagctt cacactttat acctattgat tcaattattt ctttacatga attagaagct
     5941 catttccctg aactcattgc aataaaagat caaaagcttg tacccacacc caatctgatt
     6001 caaatggcaa aggaatactt caaccgctat tcactcaccc gtgacgaaca atgtatgctg
     6061 aaaggatggt ggcagcttgt tattgcgtaa cattatatat cttttgccag gtatctttct
     6121 tttcattacc tgcgccaaca aaccgctgat acttattaga gctgagtgtg gcagcagtgt
     6181 atcaatgaat ggaactatcg ccaatgattc ccttacctac accttcaagc catgtactca
     6241 ttgcaggtca ttgaaagatg taagcaatta catctatttt cggggtgaca cactatgctt
     6301 tagcatacaa tgcaatcaga atattgaatc ggcaaccgta acagcctatt ttgaaaaccc
     6361 aaccacacat acaacataca aagctgaacg actggaaaaa gtaacaactg ctgtatatgg
     6421 gttttcactt ataggcagcc ttcttgagac aatatactgg gatgatattc gcaacacaca
     6481 tagtattaaa actttaaaac taccctttgt tatccatgtt actattcaaa aacaaaatca
     6541 gaaggtacac catacctttt ctgatacatt atatgtgcaa tttgttgact gattattttt
     6601 gaagaatgcc acaggaaatg tcgaagaata aaatatcaat aagagatgta acacaaggta
     6661 tcaatgaaaa aaaataaaag caacatgtat gtccgcttct ggggtgttag aggttcaatt
     6721 ccaactccgg gtaaggattt tgttacatat ggtgggaata cttcatgtgt tgaactcaga
     6781 tgtggtcaaa caatccttat ctttgatgct ggtaccggta tcagaatgct tggaaaagca
     6841 ctatcaaaag aattttctgg taagcctgtt acggtacacc ttttcataag ccatactcac
     6901 tgggaccata tacagggatt tccgtttttt gacctggcat ataataaaca tgcttctatt
     6961 tacttttatg gagggcacag cgtctcaaca ctggaaaagc ttattttagg gcaaatggat
     7021 agagagtatt tccccgtaac tttatttgag ctgtcatcta acatccattt cgttgagcta
     7081 tcgcacaacc cattcacaat aaacgatatt acaatatggt acacccatct tttccatccg
     7141 ggattatcat tgggtttccg tgttgaatac aataaccatg tctttgtgta tgccagtgat
     7201 aatgaagtat taaatgatcc ggagttaggg gattataacc ttaaaaatat aggtaattta
     7261 atacaggatg ccgatatact tgttgctgac tgccagtata caccccagga gtatgcaaca
     7321 cgcataggct gggggcattc ttctattgaa tccgttgccg gcgttgcttc acgttttaat
     7381 gttaagcatt taatcacatt ccaccatgat cccaatcata ctgatcgtga tattacccga
     7441 gcagtaaaga aagcacgttc attgtcttta cccatcagaa taactgctgc aaaagaaggg
     7501 gatattcttt atatttaaaa aatattgatt ttatattttc tttatctata gtggcacatg
     7561 tatgaaatca attgtgttac ataatacata cctttgcatg cgccatgccc agagcactgc
     7621 caacgaaaag catattatca tcagtgaccc tacaactggt ataagcaagt atgggctgac
     7681 aaaggaaggg gaacgtcagg tgttagaggc aatagttact aataaagaaa tgcgcaatat
     7741 atcatacatc tatacatcgg attttttgcg cgcatatcaa actgctacaa ttgtttcacg
     7801 gctttgtaac aatgttccta tcataactga tgcacgactg cgtgagcgtt attttggcct
     7861 gtttgaggga accaacagcg aaaattacca caaagtatgg gatatggatt gcacatcaga
     7921 atttgatgtt gcctgctata atgttgaacc tattcattcc gttgcacgac gtatgctatc
     7981 atttttactt gattccgaaa tacaccctac caatcaaaag attcttgtgg tatcacatgg
     8041 tgaccccttg caaattctat atgctgttac ccgtggtctt gctgcacatc agtttaaatc
     8101 tttaccacat tttgcaaatg ctgaaatacg catattgcca tcacacttta caattccgga
     8161 ggaatatcta tcatgaattc accctggctg cctttcatat atcagtacag tattttcacc
     8221 atattcttca ttttcaccac aatgatagct ataaagaagc atgctctatc actttctcat
     8281 cctaccgagc gtcgtatact ctttgaaatt attgcaggct atcttttttt tatcgtattc
     8341 catgcatcaa tgattatcgt tgccggagtg taagacatga ttaagggtac agtacttgat
     8401 tttgccatta ttgtatgtta ttttttgttt atactgggat ttggaacatt ctttggcaaa
     8461 tatgtaaaaa gtacaaaaga tttctttttt ggcggtcaac gttttagctg gtggcttatt
     8521 ggatttagct gcgtggcaac aacggtaggg tcatacagtt ttattaaata ctcagctgta
     8581 gcatatcagt acggtttttc ttctacaatg acatacctca atgattggtt catcctgcca
     8641 ctatttgtat tcatatggct tcctataata tattattcac ggataacatc aatacctgaa
     8701 tactttgaaa agcggtttaa cagtaccaca cggacaatca gtgtatttat acttttagtg
     8761 tatttattag gctacatagg tattaatctt tatacaattg gcgttgcatt taatgccatg
     8821 tttggctggg atgtcatgat tactgctata ctgatatcct ttgtgtgtgc agtatacatg
     8881 catgcaggtg gtcaaacatc agttatcatg accgaccttg cacaggcatt gatgctgctt
     8941 tcagccggat tcctggtatt ttttctggga attgaaaaaa ttggcagtgt atctgccttc
     9001 tggaagtctt taccctatga atttaaattg cccttttcac cgttcaacaa accacccgat
     9061 ttcaattttg tgggcatatt ctggcaggat gcgtttggca gttccatggc attctatttt
     9121 atgaatcagg gcatactcat gcgtttctta agtgtccgtt ccccccgcga tggcaggaaa
     9181 gcaatgtttc tggtttgtct tgtactcatg ccactggctg cgtttgccgt ggcaaatgct
     9241 gggtggatag ggcggtcaat gcaaaattta ggactactat caaacagtac tgaccccaat
     9301 catatatttg ttgatgtagc ttttggaatc acaaaaccag gaatctttgg atttatcatg
     9361 gcagctttaa ttgcagcact aatgtcaaca attgacactc tgattaacgg agtggctgca
     9421 attggtgtta atgatattgt taaaaaattt gtccctgata gggaagatac gttttaccta
     9481 aaatgggcac gtaccatttc tttagctgct gcagtaattg gtgttgcact ggtaccctta
     9541 ttcatgcagt tcaaatccat ttatctggcc catgggacgt ttattgctgc agtaactcct
     9601 ccaatgatag taactatctt atttggcatt ttatggaaac gatacactac aaaagctgct
     9661 atctatactc tggttattgg cagtatagca atgcttatct ccatacaatg gccatcagta
     9721 ttaacattgt tatcccacgg tgttgcaccc gaagggctac agtatttacg agcgctatat
     9781 ggattaatag tttgtaccgc tgtcagtatt gttattacag tactgtcaaa agaaaaagaa
     9841 ttatctaaca ttcccggtct tgtcgttgat accattcatg aaggaaaggt tttatttaaa
     9901 ggcggcgaac ctgatgattt tgatgaaggt aaaattatcc attgctacat agccgagacc
     9961 agtgaaggtg tttctttaag tacaaagatg atggaatatc tgcaggctaa acctggtgat
    10021 attctttata tagccgatga acgctggtgg ttaggaggat tacgatccat ccatgccaaa
    10081 gccgggacgc ctcatcaggg agttgataac gtactatttt tagggtcgga tcttattaca
    10141 cagggaaatt tagatataaa caggaaagta actgtcataa aaattatcta attatatatt
    10201 attttttctt tgtaataaaa gctgcaaatt tttgagcaaa tccattatga acagaaaaac
    10261 cgctaagatg ctgtttaaca tcttcaatgg taataaatgc atttggttcc agcaggtgta
    10321 caatttcaac aactcttttc acatctttct ttggcacaac tgcaaacaat aaggtgatag
    10381 gacctttcat gccaattcca tctatctgag taacaccaaa tccttcctgc cgtagcacca
    10441 gcggcaatgc agaaacaact ttactggtaa ttattcgtac tgattggtaa ccaatggcaa
    10501 ttttttcttc aaggagcatc ccaacataat taccggtagc aaatcctgca gcatacacaa
    10561 tataactgat tgcaccatca aaatgcttca aagccgaatt aatagccaca agccatatta
    10621 aaacttcaaa aaatcccaga accggagcaa ttgaccggta tccacgagcc attaaaataa
    10681 tgcgcatggt acctatggat acgtcaaaaa tacgggcaac aaatattaaa aacaatgtcc
    10741 cgtaatttga aataaatgta ttaacaatat ctgtcataaa ttccatggta aaaaaatatt
    10801 gtttgatagt tttttttcaa taactattct ttaaaatcag tgtcttatga atatacgcat
    10861 tcaataatag acaatacatg tgtatcacgt aacttttttt gtaaaaaatg tttatttaag
    10921 attatcttta gttaattttt ttagtatatt tcttgaaaaa atatatatac tattctatgt
    10981 caattgtatt tttcaagtac ctacggagtg aaggaaggtg tcaggatatc tcattggaat
    11041 tttagcatat attatattcc agcttatcct agggataata gtatcccgta agatccattc
    11101 cgatgatgat tttatccttg caggcagaac attaggctac cttctggtaa cattttcagt
    11161 ctttgcaaca tggtttggtg ccgaatcctg cattggcacc tcgggtgcgg catatcagga
    11221 cggccttgtt ggggttactg ctgatccttt tggatatgcg atagttctct ttgtacttgg
    11281 cctatttttt gcatcccgcc tgtggaatat gaaactaaca actatctccg attttttccg
    11341 gaatacatat gacagtactg tagaaaaact caccgcaatt attcttattc ctacatctct
    11401 cctctgggct gcagctcaga tacgggcatt tgggcaggtg ttatctgcat catctgaact
    11461 ggaattaacc atttccatta ccatatctgc gatagttgtt attgtatata ccggattagg
    11521 tggattgctt gccgatgcaa taacagatat tgttcagggt gccattttaa ttattgggct
    11581 ttttgtcatc tttttttccg tacttggtga tgttggtgga ttgtctcaag caataggaat
    11641 gatcgataca acccgattaa cctttgtacc tcattcaata actaatccct ggcagcgttt
    11701 attgacaatg gcagaatcat gggctgtacc aatctgcggc agccttgttg ctcaggaggt
    11761 aatctcccgc atgttagctg cacggtcagc taccgtggca cgcaggagct cattattagc
    11821 ttctctcatg tatatctcta ttgggatgat acccctgttt atagggcttg ttggctatca
    11881 tgtcatgcca ggcttgaatg atcccgagca gatattacct aagatggcac aatttcattt
    11941 tcataaatac ctttatatca tttttgcggg ggcactggtg tcagctattc tttccactgt
    12001 tgacagtgct ctgttagcat cttcagcact tctcaatcat aatcttattt tcaatttcta
    12061 tcccaattta agtgacagag caaaactcat agccgacaga actggagttc ttctgctggg
    12121 gatcacatcg tatattttag cacttcatgc agaaggcatt tatgcacttg taaaagaagc
    12181 atctgcgttt ggcagtgccg gagtttttgt tatttacatc attggcatgt attcacaaaa
    12241 ggccagtgta cccgctgcga tagtaaccct gataactgga gctacaacat actttattgg
    12301 gaaattcttt attcactttg aatatagctt tttgttgtct gttttgattg ctgttttgag
    12361 ttattacgct gttatgcttg taaccagggt catatcatct gctgaacttc ctgttgcagt
    12421 aaaagacacc gatagttaaa aatttcttgc atttgcatgg ccaatatgct ttttataaac
    12481 caattgattg aatatattaa gtacatttaa ttaatgttaa aaggtacacc atgaaacgca
    12541 aatatggtat tattgtatta atcctttttg tgattataag gcctctgcat gcaacagtat
    12601 tttctcatgg agcatacagt tcccatacat catttcatct tgttacggat gacagtacaa
    12661 caaataaaga acagccccca tccaaaaata accaggacga acatccacag gatgatagtt
    12721 caaatgatga tagcatggat cataatgaca gtgaatctga tgaggattta gaagatattc
    12781 ctgatgattt ttatgattat caggaagaaa atttttaagc ccttttaatg accaggcatg
    12841 gtattggtga tttccgtact actttttcag tgactgaacc caataacatt tcttcaatat
    12901 tgcttttgcc atgtgaaccc attacaatgc atgaaacatt atactgctgt gcagcatgta
    12961 taatttcctt aaaagggata ccttctacaa ttaaaggagt aactttaaat agtcgtgaaa
    13021 atttcttgat taatgtattc atctgggttt cagcttcttt tttcaggata tccgatatat
    13081 catacgggta gacaaatgtt tcaccaaatg ctgttgtgtc tattattgta ctctggcgta
    13141 tgtcgataac atggaggatg attaattctt ttgttccagc tttcttcatt aattttatat
    13201 atggtatgga tgcatgagca cattcagaaa aatctgttgg ataaagaatt tttttaaaca
    13261 tactggtttc cttttaatga aacatacttc tagagtagtt atagaataat actatgagcg
    13321 atagtaatca agaaaaaaaa tccccttttg atgattataa ctttgctgta atgtatgaaa
    13381 agcgaaatgc ccaatttggc tacccaggca agcttttaga tcatattatg aaatatctca
    13441 aacaatacaa tgtgacatca gttgctgatg ttggagcagg aacgggtgcc ttcagcattc
    13501 cgcttgctat tgctggattt acggtgtatg ccattgaacc cgctgctggc atgcgaagta
    13561 ttttagatga caaaataaaa aatctttctg ttaatgtaac tatctatcct catactcttg
    13621 aaacaattaa agaactgaaa gctgattcct gtatagctat gcattcttta tatggtatca
    13681 aaccgttaga aaaggctttg gtagcaatga tgcacatcgc ccctttagtg ctgatagcag
    13741 tacgtacaga aaaaacatat acgttaagtg atgttatcag aagctatttt aaaaaggaac
    13801 ggtcaaaaca ccatttacaa cacattattg attatctgga aaagcattca atatcccatg
    13861 aaatacattc aatccaccag gcgcacaaag tccttattca tgatatatat caggaagcgt
    13921 tatttcattg ttatgcaatg ggttttgatg aaagctatgt atctacaatc atgacaatct
    13981 tagaaaagca tcttcataac aacggctcat actggtttga aaatgttcat gacgatgcaa
    14041 ttgttattat ccacgcctga tttaattttt tacagtacaa cagcctgtat ctttagcaat
    14101 cctctgtatt tttgaaaaaa atttttcact gctgataatg agtaatgaac cattatcaac
    14161 taccatacca tcacgcgttt caccttttat ggaatataca aatggtaata catacatggt
    14221 atcattaaac agatatatat acatcttttc gccggtatta ataacaatac catgatccgt
    14281 catatgaata tctgtaagac tggaaaagtt tttgattatt ttttcctgcc cggtttttat
    14341 attaatactt ttaacatccc agtttcctgc actccctttg ataataaaaa gtgtcccatc
    14401 atatactgca tgtttccatg aagctacctt taacgatgag ataagtacct ccccgttttt
    14461 agttattacc cacagattat acgccccagc aatacctgca atgacaatca gcttatcatc
    14521 tacaatatct atatatgcat tataatattt aaatgcttca ggtggagaaa actcaactga
    14581 agataatttt tttatttgcg caatttttgg attgtattca aacaattctt taccactcag
    14641 gaagtaggta tgtgttgcat gggcaactgc ttttatgggt tcttgcgagg ggttgtttat
    14701 aattgattct ttatcctgga ttgaaagata cactacaata ccatccgtaa caacacatgt
    14761 atcttcttcc cgatcatacc atacactcac aacttcacgg gggaaattgt aggaatattg
    14821 ttctggataa agcatagata atatattttc tgaagaaaaa atagcacaag gtgaaaaata
    14881 aagattctga gcattgatag tcttaatagt ggaatagtgt agcaatcctt ccctgaattt
    14941 tacaaatgca ctttgcgtaa gaccttcatt gtaaagccgt atcccttcat tgatttcctt
    15001 ttcgcgagaa caggaaactg ttatcaacag catacacatc agcaaataca aaaacctgtt
    15061 cattattaaa cctttttccc cttaatcttt tccatggttt tctgtaaaaa tcttccatcc
    15121 ataatttcaa tcattttatt tgctgcctgt gatttagcat ttatgctgaa ttttgaagtc
    15181 gtaataaata tgccgcttct gcatttctct tccagcattc tttgataaaa ggcattaatc
    15241 tggccttcag ttatatcacg ggttgttcgt atgaagttga ttaatgctgg aggttcattt
    15301 attcttttta cactgaagca tttatacata tactcgtttg gtgcaacctc ttttttgtat
    15361 acaatgctgt aattaagctg ggcaattatt tctgttatta ggggctgcaa ctcagacatt
    15421 gatgaagtaa acaaaaattt caaattttta ttttccagta ttgctttgta gtcatccagc
    15481 ttaagctgag tactgcggta atttggattt atgcttgcaa ttttttccca gtgatgtaca
    15541 gcctcctgaa tcttattttc catttcataa cactcagcca gcaaataccg gtaagccagt
    15601 ccttcatcag tactatcctt tacataggat aatcccttct ccaattcctc aatagcctta
    15661 ctgtattcat cattgtcaaa ataaatctgg ccaatagcat actggctttt cacaaaatat
    15721 cgcctgtcat tgcgcgccct tgatagcatg tccagcgctg ccacatagtc ctttttcatt
    15781 ttataaatca tacccagata ataatatggt tcataatttg aaggattgct ttcaattgcc
    15841 tttatgagaa agttttgcgc cagttcataa tctgccaggt taaaaaggca tattcccagc
    15901 ggtgcataaa tatctgtcag agcaggatcc atggtaactg ctttaagcaa ataatctttt
    15961 gcaccaatat aatcatgttg tcggtataac actataccaa gtcggtggtt tgcattaata
    16021 tcatcaggat taaactgtaa aattatcctg aactcttcaa cctccttttg ccattgctgt
    16081 gtttcatggt acaggtcggc aagcatataa tggatatcaa gttcattaac atgcttattg
    16141 aattcaggaa gggcaagaat accattgagt tcggaaattg ctaaaagata ttgtttttgt
    16201 tttactaaaa tctgtgccct taagtaccgt gcgggaacat aatctttttt tcgttcaaga
    16261 acaagcttga caatttcact tgcctttgcg atgtctccac tatcaaggta ttcctgagct
    16321 ttacgcacct ttgcaggata ctggtgagca cgtataacta aaagcaatgc accaacaata
    16381 gatgcaccca caataaaaaa tgttatactt agcagtgaca tacaattttc acatttaaaa
    16441 tttgatttta ttactgcacg cattatattt taagtcaagc attatagtgt gccttagttt
    16501 ataataaata attaaaaaga aaggaacata acaatggcag aagtaggaat tatcgtgggt
    16561 agtgattctg atttaccaaa attaaaggga tgtttccaga cgctggatga ttttggtatt
    16621 tcatacgatg tgaaagcata ctcagctcac agaacccctc atctggtggc tgagtggatt
    16681 aaactgatgg aaagcgctgg agctaaggtt tttattgcag ctgctggtgg agcagctcat
    16741 cttccaggcg ttgttgcttc acacactgtt ttgcccgtca tcggtatccc tattgaaacg
    16801 cagctgtccg gcggccttga ttcgctttta tccattgtgc agatgccatc aggaattcct
    16861 gtggcaacag ttgctaccgg taaagctggt gctaccaatg ctgcattgct ggctgtatct
    16921 atcttagcaa ctgccaatgc tgcttatcag gaaaagctgc gtaactatcg caacactatg
    16981 caaaaaacca tcattgaaaa aaatgaaaca ctttccaaac aaggtttata cgaatacata
    17041 aagtcattag aggcaaagaa atgattgagc acggtattgg ggtagcttcc cagaataaag
    17101 tactcatttt agattttggt tcacagtata cacaactcat tgcccgtaaa atcagggagc
    17161 tacatgtgta tgcagagata gttccctata ctatacctat agatgaaata aaaaaagaaa
    17221 atcccaaagc tttagtactt tccggtgggc catcatctct ctatgaagaa gactccccaa
    17281 aaattcgcaa agagttattt tctttaggaa taccagtatt gggaatatgt tatggattgt
    17341 acgtggtcgt tgatgctttt ggtggagtta tatccccatc tccttttaaa gagtacggca
    17401 gggcagatat tacaattgtc aaagattcac cattgtttaa aggattgcct gatacacaaa
    17461 ctgtgtggat gagccatggt gataaagttg aaagcgttcc acaggatttt actattattg
    17521 caggttcacg gaatgcgaaa attgcagcta tagaatacag gcaaaaaaag atctatggcg
    17581 tgcaattcca tccggaagta taccatacac aatatggcaa ggagattttg cataactttc
    17641 tttttgaaat ctgcgatttt aaaccaacat ggacgatgca atcgtttatt gattcagcta
    17701 tcaacgacat tcgaactgtt gtgggcaatg gtacggttct cttagggctt tccggtggtg
    17761 ttgattcatc agtcactgcc gtgctgttac aaaaagctat cggcaaccgc ttatattgtg
    17821 tatttgtcaa taacggcctg ttacgcaagg acgaacatat aaaagttata gagcgattta
    17881 aaaaacacct taatctaaac cttatctatg cagatgccga ggaattattt ttacaaaaac
    17941 tcaaaggtgt taccgatcct gaacaaaagc gaaaaattat tggcaaagaa tttattaatg
    18001 tattcaaagc tgaagctgca aaaattggaa catttgattt tttagcacag ggaacgttgt
    18061 atcctgatgt aatagaatca gtttctacca aaggaccttc tgatactatt aaaagccatc
    18121 ataatagggt ccctgaagtg cttgaattga taaaagaagg caaagtaata gagccactta
    18181 aagagttatt caaggatgag gtgagggaat taggcaggca attaggtatg cccgaagaac
    18241 tgataaacag gcatccattc cccggacctg gccttgccat ccgcattatt ggcgaagtaa
    18301 caaaagagcg tcttttcatt ttacgggaag ctgatgacat cctcattgaa gagataaaaa
    18361 aagctgatta ttacacaaag ttgtggcagg catttgccgt atttttaccc ataaaatccg
    18421 taggtgtcat gggcgacaaa cgtacctatg aaaatgtcat tgccataagg gctgttacgt
    18481 cacttgatgc tatgactgcc gactgggcat atttgcccga agacttaatg cgaagaatct
    18541 ccacacgcat aatcaatgaa gtaaagggcg tgaacagagt tgtatatgac atttcatcaa
    18601 aaccaccatc aactattgaa tgggaataaa attttttgat agaaaccatt gacgaaaaat
    18661 aaaataatta ttatatttta aaattaaata atattcttaa acatatgtaa ttctttgatt
    18721 gcagagtttt tgttgtttta gattacgacg ttaaaacggt tacattattt tttacggagg
    18781 accaaacgat acatgttgtt acgcgattat gatgcagaac aaataaaaga tgaaataaca
    18841 cagtgtgtgg caacaaatgt ttttgattca gaaccagaaa gctataccaa agcaaaagaa
    18901 ttgttgcagg agcttttcat agtttcaggt ggaaatttta acaggcctga ttacgaatca
    18961 ataatgaaag tattaaagct tattgctgca cgccagctta aaaaaggaaa agatacaaaa
    19021 gcgatagcag agcgttttaa agagatagct aaaaagatag aatcggtagc agtatatacg
    19081 taattaacgc atgcgcttgt aacgctgcag ttctctgtca gcatcacgct ttttaatatc
    19141 atggcgttta tcatacaggg ttttaccttt tgcaagtccc agaaggactt taaccttccc
    19201 atttttccag tatagcttta gtggcaccag cgtcagtcct ttttcctgct gcttaccggt
    19261 aaggcgcttt atttcctgtt tgtgcagcaa aagtttgcgc acacgtaatg ggtcgtggtt
    19321 aaaaatatta cctgcttcat acggcgatat attcatccca tataaaaatg cctcaccatc
    19381 cttgatgcgg gcataggaat cctgtatact gacttttttt tgacgtattg attttacctc
    19441 agtacctttc aatacaattc ccgcctcaat agtatcaagt atctcatatt caaagcgggc
    19501 ttttttattt tgaattatct ctttttcctc tttcatatgt tcaatccatc acagcgaaac
    19561 tatgtcaata aatgctatgt attccatggt cgtttaacca gtggtaatgc ggtgcctaag
    19621 gctattacca ccgtacccaa ccatactaac catataagcc gtttaaatga tatatcaact
    19681 attacaaaat caggtgggac tgtagtgttt gcaggtgcat ccatatataa cacaatgagc
    19741 ttttcatgaa tatcaaaatc catcaatgaa atcgtacgcg ttgtacccgg tagcattgaa
    19801 tttacgtttg tgcgtctgtc gccatcaaat agtactcctg ggcgtaccgt atactcctta
    19861 ccctgatatt ttatggtgca caatgcatat atttttggat taccctgcat cattgccatg
    19921 cggtcaatat caaacccatc aaagcgcacc gtgacatcat taaagctacg cacctcacct
    19981 tttttcagca tgatctgtgt taacgtatcc tttcccaatc tgtattcaac aggtgaaata
    20041 tatacatcat acccaatatt gtaatcaata tatggctctc tgaatagctg attggtgcga
    20101 gtatcaataa aatacggtgt ttgaatagtc tttgacgtgt tgtaataatc atacttgaat
    20161 acaattgcac ttttaatgcc ctcaaccatg cccacaagtt ccagagttac tggatatact
    20221 gtcttttttt cattctgcgc aatctgtgca tgatattcaa ccgactgagt ttgtgacgta
    20281 atgattccca caatgagtaa tgctacacca ctgtgcgcta tacgtgcagc taagctcaac
    20341 ggtatgtgtg gcactatacc tttaacattg gtaagcagta taccaaaaga aagtaccatg
    20401 agaataaaag caacaggtac aaagggcttt ccaacaaata aaagcaatga tactgtaagg
    20461 gcgatagttc ctgctataag tactgttttt aaggttggtt ttctggcaaa aggtgttaaa
    20521 agcaacaaca ctacaataag aaaacctatt ggccttgcaa tacttacata atatgattcc
    20581 tgtactgttg atgcctgccc cagtaacccg gtgatgatgg gcatacaggt accaataagc
    20641 acaaacagcg caaaaaataa taatgcggca atgccatata ccatgatatt gtcttttgca
    20701 aacaatgtat tatctaatgt ttttccgatt gcatcatttc tgcgatacca caacaatcct
    20761 gttgctgcaa taccaattgt tacaataaaa agcagaagat cagtaaaatt acccgcacca
    20821 ctgaatgaat gcaccgaaaa atcagaaagg acaccactgc gggttaaaaa tgtaccaaag
    20881 ataaccagta caaaagtaac tatcgccatt ataatattaa acctgataag tgccttttta
    20941 cgtttttgca ccattacacc atgcatgagt gcaaggcctg taagccatgg aataagtgaa
    21001 gcattttcaa ccggatccca tccccagtag ccaccccagc ccaatacctt gtatgcccag
    21061 tatccaccca aaaatatacc ggcaccaagc gttaatacag tagcaacaac ccagcggtaa
    21121 cctgttgtca taacagaata atcgttatac agcagtgcat ttactgcata cacaaatggt
    21181 actgttgcca gtgcataccc ggcaaaaagc accggtggat gtattaccat ccagaaatcc
    21241 tgtagcaagg gatttaaccc catgccatca tgtggtataa cccctggtgc gacatttttt
    21301 gaagcatccc atacatacat aaaggggttg ttatgcaaac aatgtatgat aaataccagc
    21361 gttaccagcc ctgccagtgt caaaaagctc tgtacatggt gtttttcata tcgcaatgca
    21421 agtactactg ttaccataac aaaaaacgtc catagcaaaa atgagccttc ctgcccagcc
    21481 cacactgctg caatacgata caaaagcggc aaatccacag atgagtgttg atacacatat
    21541 aaaaattgaa atgtgttgag cacaaacgca atggttaacg caacaagcat aaaccctata
    21601 acaactgttg caccatagta actgtaatgt gctgctttta acaggttctc ttttttaaac
    21661 gatttaacag ctaaaataac cgaggccact accagcacaa aagaaagcca tgggaggtaa
    21721 tacagcagat tcattattta ttctccttgg tgtatttaga agggcatttt accagaattt
    21781 tatctgcttc aaatatctgt ttttctttat tataggttcc aattgcaaca acgcttgtgg
    21841 cgtgttcaaa gttaagcggc ttggatcctt tatgaatcac agtcaatcgt ttggcattat
    21901 catcctcaat ggtaaatata aagccgtcag cagaattttg caccggaact gacttcacca
    21961 gttttcccag tacctgcacg tatgcttcac cttttgccgc ctcatcaaat gatacatacg
    22021 gcgttaatat ccccttaaaa gaaaaaagcg caacagcacc gcatactaca atgatactta
    22081 ctataataat attacttttt ttcattgagc ctcttttcca attttttaac cttattatgc
    22141 aaaagcagca agtaacacac aatgccaatc cacacaacgg cggataccca gaatagcact
    22201 gtagtagggc ttttctctat ttgtgttatg ttttgtgcga tagttaccat aaaaagcagt
    22261 ttcatgatac atcctcagta catacatatt ctagcttata tacttttagc aaaagtttta
    22321 gcacaattgc ataaaaaagt gtaaacgtca caaccgagat aataagaaca agccgcattt
    22381 cagtatccag aaggattgct ccttgagtat taatcacagg ttcaggatga agcgatggat
    22441 atagccgcgg aacaacaaat attaaaaatg gtgaagcgac aaacgctata acaagataca
    22501 cactttcaag catagcacct tttgggttag tatgcacaat ttgatgcagg ctaaaatacg
    22561 ctatatatac caatagcagg agcgtgattg acgtttcacg tggatcccag ttccagtaac
    22621 ttccccacat aacctttgcc cacagtgaac ctgttatcac ggtcaatatg ctgaaaaaaa
    22681 atccaatgtg cactgaactt cgtgcacaat gaatatactc tattgcattc tttcgtatgt
    22741 actgtattga agcaagacct gcaacaataa atgccagtgt tgacacccag gcaagtggta
    22801 cgtgcaaata cactatcctg cttgtttccc caaggattgc tgcaggcggc acccatatat
    22861 atatgagata cagcgttgcc agcattaaca gtgcatcaat tatgattaaa accatgaaaa
    22921 actccatgag aaattaagat tcacctaaaa ttctctccat atatagggga acagtatata
    22981 tgataccacg ccaagcaatg ttgaaaatgc aagtagaaaa agtatagcac tccatgaaga
    23041 ataaatctct ttagaaaaac actgtgttgt agattttatt acgactgtga taatgggtag
    23101 cgttatgggg aacgataaaa tagtaaaaag gccgcctcgc gcctgtacca gtgcggttat
    23161 agccgcgatt atggttgtgc acactgccag agcactactg cctgccagca ccatgattgc
    23221 aaaacctgat acattataca ctgaaacatc cataacaaac agatacagca atccagctat
    23281 tgctgcaaca gccaccataa taatacaatt tacaataagc tttgaaacaa atatgccttc
    23341 agggctataa tacatcaggt taaagagtgc agtatgttct tctatctctc tgtaaaagag
    23401 gtgagaggta ttcatccctg aaaaaaatat aatgacccac aaaagaatag catgcaccat
    23461 tgggctccca attatgccac ctgaagcaaa accaatagaa attgtcacaa tgacactgaa
    23521 cgatagtatc agcgaaatga ctaccttgct tctgaactct atacgaagat cttttacaat
    23581 attacgcacc aatcccttta tcaagcacaa tactcctgtg acaaagtttt gcttccctgc
    23641 tgtcattagt tgccatgata atagcagtaa atggttgtat ttcttttatt agttcaaaaa
    23701 gaatttttct tccactatca tctaaatttg aacctggttc atccagcaat aaaacttttg
    23761 gttttttcaa aagtgccatt gcaatcttgc aacgttgctg catgcctgta gaataggtat
    23821 ggagaacttt atctttatgc tgatacatat ctaaacgatg tagaagttct atagcatcat
    23881 cagttaccga gccaaaggca aagataatat tttctgttaa tgtaagatga ttgtaacact
    23941 gtatgtaggg agcaacaatg ccagcatagt ggataaccgt atgggcaggg atggattcca
    24001 tcttatcaaa atactctacc ctgccactat caggtgacac aatatttccc agtattttca
    24061 gcaaggttga tttgccactg ccattgggac caacaattgc aacagattca cctgttgata
    24121 ctgaacatga aatattgctg aacaacctgt agctatagcg ttttgatata ttgtgagctt
    24181 ttattgtaaa cacaaaacct tacagaacta tttctttttt agctgttgcc atttcaataa
    24241 caggtaattt tactgcctta tctttcataa cctcatccac tgtcttctga tcagctaatc
    24301 ggtacagtaa tcttacctgg atgctatatt gcccttttaa tcctttgggc agtatgatag
    24361 tattggtagc cgattctttt ggtgaaatac gattatcaaa cagaatttct ttagcttttg
    24421 caatgtttgc aacaggtttc cctttcccat caccaaaaat agtcttaaag attatagtat
    24481 tatcagggag atagcccctt ttatcagcct tcccaattga aaaaacctct ttgccatatt
    24541 gattcaggat aactatctcc aaccacattt gccgaacatc agttagcccg gttggaagtt
    24601 tatgccctgc gccggtattt gttatagtaa ctgcaaaagc gccggttgaa atatcgcgtg
    24661 tatcaattaa aatagttgcg gcatttttca gccgttcttc agccattttg cctttgactt
    24721 tatcattata caatcctggt atgatactat tgccaccaac aaaataatga gtaaatatgt
    24781 gatctcgctg tggcccacca aaagcagcaa atccgggatt gttggggcgt ggagtagatc
    24841 ctgttgccgg aacgcctggc ctgtgataca tatgacatcc ctgacagggg acagtggttt
    24901 tttcatcgcc agtattgtat gggcttttgc tccactcttc aaaggttgtt tccagttttg
    24961 tattatagac tacatgccta acatcatggc atgtaccaca gaatgcagcc tgcgtgtgaa
    25021 atttggaata tgctgcctga tgccagtcag gtgttgcatc cttaaaaggg ccatacttta
    25081 caccgggatc tgcttcacca ttgcctggct tataggtaaa atgtgcatta tagtttttct
    25141 ttgcatcaat gattacatgg cagtaatcac actgaattcc ttcttttgtt atttcaggca
    25201 attcgttttc cattgccagt tcttcagtca ctgttactgg tttgccggta ataaagccta
    25261 ccggtatatg gcacttaaca cagctttctg cctcttcaag ttcatcctta tttacaggat
    25321 tttgtttttt tatatcatct aaataaaatg cagcatactc tttgtagatt atatcctttt
    25381 gtgcaaggtt atgcattgag tttttccact gttcatatat ctcatcatgg caaccaccac
    25441 aggttgcagg gcttataaat tgtgctaatg tgtagtgggg tgttgattta catccggtaa
    25501 aaaaagcata tacggttaat gcaaccatgc tgattataat gactattttt gatttcatgg
    25561 aactgtctcc tcaaaactat gatttacgga tactatgcca tcctgaggaa tcccgacctg
    25621 tcggggtgac gaagaatctt gccgtttaaa aatccagatt cttctcctga tttatcggga
    25681 tacaaaatgt aattaatact tctgtagtaa atcaaaattg tcaacaatgt ttattatttt
    25741 acaatgtgaa caactcgctg ggggaatggt atctcaatac cttcctcctt taagcggtta
    25801 aatattcgct tacgcaattc agactgaacg ggaaactggt cagcgtattc ttttacatac
    25861 actataaggg taaaatccag ggaactatcg ccaaaacctg gaataaaccg tactacaggc
    25921 tctggatcca taacaagctg ttgtatggtg ctagctgatt ttttcacctc atcaagtata
    25981 acatgttcaa catgctgagg gtcggagtta tagctcacac ttactggaat cctcagcgcc
    26041 agatgtggca ccggaagata aaaattggta atcatgctct gagccatctt actgttggga
    26101 acaataacaa tgttgttagg cagcattctg attcgtgtag ttcgccaggt gatatcctca
    26161 acatatcctt caatgccttc atcaattttt acaaagtctc caacccttac cgacctttca
    26221 atcaaaatct gcatccctgc aaaaagattt gacagtgtat cctgcagtgc aagagcaact
    26281 gccagaccac ctactccaag agttgtcagt ataggagcta tcgatatacc taaatagttg
    26341 aggatgatta aaatccctat aagtacaaat aaacccttga tgataatata ggttaagcca
    26401 gtaggtgcca gcggtatatt tgcaatcttt acgtatttct gtaataatga tccaactata
    26461 ttagcaatgc caagagtaag agcaaaaata ataataacat gtacactttt acttatcagc
    26521 atttgccatt tttctgaaag tattgtgtac tgtgaagcaa tgagaatggc aagagcaaga
    26581 acgacaaaga atgcaggttt tttcaatcca tcaataatta tatcatcaat atcccagctt
    26641 gtagcggcag cccattttgt taaaagcgaa aacactattt ttttgaacaa taacaaaaca
    26701 gtaaaagcaa caccaaaata cactaccggg ataaaaataa aaaaatattc ttgtagtaaa
    26761 tccatggttt actcctctga ctattgaatt accaaaaata taacattacc aatctttata
    26821 aaataaatca taaaaattgt attgattttt ctattagtat tgcaataata attttttatt
    26881 aaatatcctg taacaaatca tttcccaata caacctcttt tcctattgag gtatatacta
    26941 ttttcaggag gatatctatg aaaaaaatac ttaaatgggt ggcgatagtt atcgggagcg
    27001 tatttgcact gataattatt gcaagcattg tacttatgct ggttgtgact aaagatatga
    27061 ttgcccagca gatggagaag gcactgaacc gtcatgtgac cattgaaaag attgacgtca
    27121 gtatattcag tgttgtttca gggattgaag taaagggtgt tgccatatca aacttcaaaa
    27181 cacccaaaca gttagaagcc ctcaaagaca agcctgtaga taaaggcgac ctttttgttg
    27241 ggcttgactc tttcacattt aaattaaagt ttttgccact tcttcagggt aaatttgaac
    27301 tccgtgaact tttgctaaca ggcccaaaaa taaatatcac ccgctatcag agtggagcat
    27361 tcaatttttc tgatttaatg aagccatcta aagaggaaaa gaaggttgaa gaagttaaga
    27421 aagaagagcc ttcaaaacct ctaaccgctg atacgttacc aatcagtata acgataggga
    27481 aagttggtat agaaagaggc acaatcactt ttgttgacca gtcactgcag caaacattcc
    27541 agctatacaa tctcaacgcg cttgtacatg atattgaaat agaccctaaa gaccttgcaa
    27601 agaaaaatca ggtaaagtta aaactggata ttggcatcaa aactataggc aaaacaagtg
    27661 gtggcagtgt agaatccttt gatattgggt tggcctcacg tggaacagta aaaccttttg
    27721 acctgaagac acgtatatta aaccctgaaa tagcattaaa agcaggcagt ccttacggaa
    27781 caatgactgg tttacagata tttgatgcta tcaagagtaa tgaaacactg gctaattatg
    27841 caggcaagtt tgacttttta tcaaaagatg taaaatggaa aaatggatac gttgatatct
    27901 ggtataaagg cggattatta aaaatcacca atggaaaaat cagcaccgat gactatgcag
    27961 gcaatttcaa agctgatgta aatttcaaca caaaagc
//
LOCUS       DAORYL010000037        26225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1580_length_26225_cov_14.541995, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000037 DAORYL010000000
VERSION     DAORYL010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 26225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26225
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1580_length_26225_cov_14.541995"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            413..1264
                     /locus_tag="PLV81_08245"
     CDS             413..1264
                     /locus_tag="PLV81_08245"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOM10130.1"
                     /translation="MPLNEKVIGKKYKSDPVKISQHESIYYALAYNEDNDAYFDNRRP
                     GGIIAPPMYAVFYGAGPTAQIMFDAEIGINMMFVVHYSQYFEWIKPVKPDDEVTSEGT
                     ITHITVKEKGSILGWETVTKNQHGEVVLKAKWEFFDRSAGSGKPDEKPETETNPSQFV
                     FEDTMKVKNGQTYIYAEPSGDHNPIHVDDEFAKKVGLPGIILQGLCTMAFSHKMAVDH
                     LTPDRDPLKVRELYVRFARPVLPGQTLTFKGYTIEKLADRTRYGIVALNNDGKDVLRN
                     AWCSVAK"
     gene            1299..2045
                     /locus_tag="PLV81_08250"
                     /pseudo
     CDS             1299..2045
                     /locus_tag="PLV81_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174969.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            2299..3741
                     /locus_tag="PLV81_08255"
     CDS             2299..3741
                     /locus_tag="PLV81_08255"
                     /inference="COORDINATES: protein motif:HMM:NF020134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HOM10131.1"
                     /translation="MIGIVSYGGYIPLRRLSRMQVVQQMAWYNPLLYMLMAGERSVAN
                     WDEDSVTMAVAASFDALHLVDTKSIDAVYAASTTFPFDDRQNAAIIAEALSLQDGVRT
                     VDMSGSLKAGVTAIVAGLDAIESDSARKVLVASGERRYTRAASTLELLYGDGAAALVL
                     GREHVIAEFLGSCAYHEDFIDHYRGAEKRFDYPWEERFIRDEGFGRIIPRAIKAYCEK
                     YHTDISEFATVIYPCPFAREHATIAKKIKSSSVHTNLHEGVGDTGTAHPLIMLAHALD
                     SAKPGDKIMVVGFGQGCEVVAFQVTDAIENIKRPKGITVSMARKKDIPYAKYLKFRDL
                     IDADISIRAEVMPNTSLSALWRHRKMIHGFTGYLCKACGTPQFPPYPVCVNPACNRSG
                     QMENYSFADKPGTVLMYTGDMLAASVDPPAIYGLVEFDGGGRTLLDFTDCELDNLKVG
                     SRVAMSYRIRREDTTRGFTGYFWKAVPLME"
     gene            3752..4960
                     /locus_tag="PLV81_08260"
     CDS             3752..4960
                     /locus_tag="PLV81_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HOM10132.1"
                     /translation="MATGIKDKVAIIGMGCTKFGERWDADANALMVEAFNEAIADAGI
                     DPKEIQAAWLATCFDEVNVGKSALSAAIALRLPNIAVTRVENFCASGTEAFRAAVYAV
                     ASGAYDIVLALGCEKLKDIGYGGLPEFSQAIGILNSLWFPNITAPGCFAMLAGGYCAK
                     YGLKMEDVKRAMAHISVKSHANGALNPKAHLQRPVTEEQVLQSPMIAYPLGLFDCCGV
                     SDGAACAIVCSVDVAKALKKKDFVTVKALQISVSNGEEMAFSNWDGAHVVNTEKAAMR
                     AYQEAGIKNPREELSLLEVHDCFSITELVTMEDLHVSERGKAVNDVMDGFFDSNGKIP
                     CQIDGGLKCFGHPIGASGLRMVYEIYLQLHGRADKRQLKDPRLGLTHNLGGFPMMNVV
                     SVAIVGRYKQ"
     gene            5080..5958
                     /gene="dapA"
                     /locus_tag="PLV81_08265"
     CDS             5080..5958
                     /gene="dapA"
                     /locus_tag="PLV81_08265"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOM10133.1"
                     /translation="MFEGVFTALVTPFKNGKIDYDSLEKIIEHQIAGGVDGVVPMGTT
                     GESPTVSYEEHEEFIICVVKMVNKKIKVIAGTGSNSTDEAIYLSKAAQDAGVDGVLLV
                     NPYYNKPPQRGLIAHFEQVAKSISIPVVLYNIPGRTGINFLPESVTELVKRVPNIVAM
                     KEASGDINQMMHLIELCGDRITLLSGDDNLLLPVLGIGGKGIISVLSNLLPADVKAVV
                     TLFKAGKFEEARKKFYALLPLCRAMFYETNPIPIKAAMEMAGFCSSEVRLPLVPLSDD
                     NRKKLKDALLAYGVKL"
     gene            5955..6743
                     /gene="dapB"
                     /locus_tag="PLV81_08270"
     CDS             5955..6743
                     /gene="dapB"
                     /locus_tag="PLV81_08270"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOM10134.1"
                     /translation="MRIAICGIAGRMGKAILTVVLEKGHTLSQALDAEQCPLLGQDAG
                     SLISKKLDVSIESLRSAKLTADAVIDFSAPAATEVLLQKAVEYKIPLVIGTTGFSDAQ
                     KQKIEQAAQTIPIVFSPNMSVGVNLLFKLTQMAALVLQDGYDVEIFEAHHRLKKDSPS
                     GTAKKLIDVVMETRKDLHNAKLMHGREGIIGQRTNNEIGVMVMRGGDIVGEHTVYFIA
                     NGERIELTHRATSRENFARGAVRAAEFLADKKNGLYTMFDVLGL"
     gene            6749..7708
                     /locus_tag="PLV81_08275"
     CDS             6749..7708
                     /locus_tag="PLV81_08275"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOM10135.1"
                     /translation="MSQNDVKNDTVSFEENPYSPEIKKPEDIVRLFYIHNVPILPVIS
                     KRGILFGILRKDDVIAELSDIARSTVKIDEFVQKLAKKMTMDELLPLVVNVKDLIAID
                     LFGEVQGRMTRLDLFMAAEGHYEKKEKEKGKEEKETQPKDTVIEWLIYMVLEHIPRAL
                     YAVNSNGKTIFFNGHFEEMYINTFPESSDVDIAFTEKILMDSAINDIYTSYVYKRQFF
                     YNKELKAYYERVPLKSENKTAGYLFMFERDITKQDLLVPDIISDTDGIDELLSRAERS
                     ILVQALTQSGFDVAQASKMLHITVADLKKRIKKYDITVIKKEG"
     gene            7717..>9569
                     /locus_tag="PLV81_08280"
     CDS             7717..>9569
                     /locus_tag="PLV81_08280"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOM10136.1"
                     /translation="MAHTSLNNTINVLLIEDLNYDAQLEQTILEKSNSKYSIQWVSNK
                     EEFYKACEQCIPDVVVSDYHLPDITAEEIIEYINANFKYVPVIIISGAIGEETTVEII
                     KNGAVDVLLKQHLFKLPQAIERAIVETSKRKELQEALEKLKKTKELQELILQNVPVGI
                     IVATKDGTVIEINRHALGMLGYDTGNTPQHFSAQGFFVDSSQRNHFFKVLNAKKIIQN
                     YEARVYRKDGQIRICSFYAVMHKIDGQQYIFTAIIDVTEQKALEDRVLFLSFFDPLTQ
                     LPNRALLKDRIDLALMRARHNKRYVAVITLDVDRFKYINDSLGHKVGDDILLEISQKL
                     SKSLRDGDVVARIGNDEFGIMLIDVARNEDIISVINKIRNIFTLPFHVDTNELLITAS
                     MGVSIFPTDGNTAEELLQNADIALAKAREQGRFTCEFYTKDLNKKVSDYISLEAKLKR
                     AIAQNEFELYYQPYFNIKTRKVAGMEALLRWNSEGNVISPATFIPVLEDSGLIIDAGK
                     WIIEEVCRQLLAWKQAGLPVKPVSLNVSPIQFQQTDFKDMVMDIISSYAMDTGLIVLE
                     ITESTYMRNIDYTKKVMQVLKDAGLHFAIDDFGTGYSSLSSLLALSFDYLKV"
     assembly_gap    9570..9669
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9670..10062
                     /locus_tag="PLV81_08285"
     CDS             <9670..10062
                     /locus_tag="PLV81_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10137.1"
                     /translation="MNKNYSVKAFNASDLYTIEDVYDVKDFKKVAYMQPLKNDNMVSF
                     EKAVENIYKLHIYNYELSKAEALTDMRTKYGVQYLILMELKDWQKVSWARAIDLTNME
                     VVWVENYPAQYRDTLETVVDHFIASMSK"
     gene            10076..11137
                     /locus_tag="PLV81_08290"
     CDS             10076..11137
                     /locus_tag="PLV81_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10138.1"
                     /translation="MKKCFIIVLMYVLMLSGTVYARTTVAFGYLTNRSGNPNFNYMEV
                     IFPNSFASSIEAVYDCTVIKPLKLNELLEKKQLTLQRHYENHELPQITKAIGCDLFIF
                     GYFIPLPDNTIKIVLTLYESGSNQIFTFTNIGKMETEIFKIIDRITLVVFDFFGKNGF
                     FMSKPIMRGSSVGLITNLNPEELNSMYAEFFNAGYSVSAVQGNELDSYYAHESEIMNY
                     FQYISTDANSYDIITDWSKFTMTMGTWEGRKKKEWQEDLRKIYEIYDLHYKVFKNTVL
                     ERYKNIYPGCDYLLFVVYNPSRTSCWARAIDIKTKELIFMHSNIEASEMNANLQKTTR
                     QLITIIQTPTALPQKSKKK"
     assembly_gap    11144..11243
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11244..11548)
                     /locus_tag="PLV81_08295"
     CDS             complement(<11244..11548)
                     /locus_tag="PLV81_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10139.1"
                     /translation="MKKIIVMIITVLSSTTIAMANVYVDGLLMINNTGDAKSQSGFGL
                     KIATSIRDDINIFFRQTYSSTTEDPNTINETEYMQIQGFLGGEYVFFIQQFPVAITL"
     gene            11838..13505
                     /locus_tag="PLV81_08300"
     CDS             11838..13505
                     /locus_tag="PLV81_08300"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOM10140.1"
                     /translation="MKNRILGILFCFICIHAVSVAFAEQPDSITAIINEEQNKEHSDA
                     LLQMLQFIDSLYDNLQSRVKNGEKIVIFFDPAHGIDDDGKWEGEKTGRLSCTGLPEEY
                     YSLALSRQLYSLLKKNPYIQVVSTQDYIDALEGKTDTYNNITFRETVELAKNARAVLV
                     ISEHLNNTASIFKAYGLANVKGIHITYSNGKAYLTHIREEHKGFLTLYNKFDASGFSY
                     RVASNVKEKLLAYNLRVNSWNYGTVADDRFTYFVNYPISVIFESGFISNPDDEKILSN
                     PEYQKLIAQAHYDAILESIANVFGIDISGFWGPRIVDTADPSRNLLLLKLSRIACYYI
                     SHNDTAKGVHVLSLLLKECDPAKDAPIIENIQDMKYRIERSNELYTKALALKRKGKMK
                     RARYTMRKAISLISRSPVFYELRKAYRAEYKGLKEVIEQKSTAPRFKAHDTMVNPLAS
                     SITKPVLVVIEKNMTPQEAIFNALQCGEVLLKKISSQFVQTKIKVGKKRFEKPTQEGI
                     YIVQLTAKGNVKSVKRVAIAKLDPYKYQNQMYLKNSYCVPKERNKYL"
     gene            13474..14436
                     /locus_tag="PLV81_08305"
     CDS             13474..14436
                     /locus_tag="PLV81_08305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOM10141.1"
                     /translation="MCQKKEINICKHGNWYGKPYHFFGDYLFHTFNAKILKLSINAHL
                     GCPNRDGTVGSGGCIFCSEGSASPTATGSTSIHQQMQAAVDGFNRGMYIPRYIAYFQA
                     FTNTYATVDVLKKLYDTALAFPNVVGLMIGTRPDCLPNDVLDLIASYKKENFELWLEI
                     GMQSMHDKSLQFLQRGHTHAQTVDAITRASQRGIPVCVHVILGIPGESWDDMMATATE
                     ISRLKVQGVKIHHLHVIKNTPLEVLYMSGNVPLLSFKQYVSYVCDFIERLPGDIIIHR
                     LLGDQPKDMLIAPAWGLHKGTVLKAIEDELLRRGTYQGFLCDSN"
     gene            14513..15301
                     /locus_tag="PLV81_08310"
     CDS             14513..15301
                     /locus_tag="PLV81_08310"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005500.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOM10142.1"
                     /translation="MNLRPDTYARSTLERYTGSSIDDFGEYIILCNFQRYVDDFASMT
                     GAKVSAGYWSCAHSRERNISIINFGVGSPSAGIVVHCLSYLDKIQTVLMLGMCGGIDD
                     NLQVGDFLIPTASVRDEGTSIHYIPKDVPAQPSFWINRICEKVITKETGVAPKTGIML
                     TTDYRMWEFDNEFIDYILKHRILAIDMEIATLFAVAYAMNVPIGAIMLISDLPLKKGG
                     IKSKESASLIFNAFTQKHLQLGIKVIENIQEKSGEGITRLRSEW"
     gene            15347..15841
                     /locus_tag="PLV81_08315"
     CDS             15347..15841
                     /locus_tag="PLV81_08315"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOM10143.1"
                     /translation="MIKRNIFFITFFLFISGCASTSIPVTKTYEDAANVLANQLKPEL
                     YNLSEHKNIKIAILPFGGPKGKPTPFGKKMATLLQSKLFSQNWNLIERDQIDKLINEK
                     NLSATGIVEDKDYMSTGSIAGADFIIVGTTYYGKKALITVKIINVHTGSIEGIAQVYL
                     TFSK"
     gene            15857..16363
                     /gene="tpx"
                     /locus_tag="PLV81_08320"
     CDS             15857..16363
                     /gene="tpx"
                     /locus_tag="PLV81_08320"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOM10144.1"
                     /translation="MPALKITANGNPVTLEGQMPSVGDKALDFTVLDINFAPVSLLNF
                     KGNIIIISAVPSLDTSVCQLQTTRFNQEAASLDAVVLTISMDLPFAQKRFCESFNIHN
                     IKTLSDFKDKSFSLSYGIYIRELGLIARSVWIIDKAGTIAYRQIVPDITQEPDYDDVL
                     KAARALGA"
     gene            complement(16416..18812)
                     /locus_tag="PLV81_08325"
     CDS             complement(16416..18812)
                     /locus_tag="PLV81_08325"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOM10145.1"
                     /translation="MNRGKKVIIGLIILILVLAVAVFLYVNNLAKSGLPQYSGKLQLK
                     GLSQAVNVYRDEYGIPHIFAKNEKDLYLATGFVMAQDRMWQMDLLRRVTTGRLSEIFG
                     KDLVDTDVLMRALQITKKSNYVIAHTDPTVISHLQAFADGVNQYLELHAENLPLEFKI
                     LGYKPENWKVEDSVNLIGYMAWDLTMPWTTETVLYKIWKKVDQVHAKALTPDVKYITD
                     VIYPSFAKARHLPELSNTILNASQKLIEIGAGIFSASNNWAVAGKKSITGKPILANDM
                     HLGLFIPGIWYQMHQVVEGKLNVTGVAIPGEPSIVCGHNEHIAWGMTNVMVDDMDFYL
                     EKVNPNNKMQYQFNGQWKNFTVVKEDIKIKGGETVSKEILFTHRGPVVTEFKKTNDVI
                     SMRWMGNEPSNELLTVYMLNRAKNWNDFTSAIRHFRSVSQNIVYADTSGNIGLYCAAG
                     IPIRKGNPMMVNPGWTDAYDWKGIVPFEQQPHVYNPKEGFVASANNKTVMEQYPYYIS
                     YWFEMPWRYNRITKLLQQKVKLSVNDFKAIQTDQVSELAIIWKPLLLQIIEGDSSLKE
                     SLKDIYTILKDWDGSYTVDSSAATLFDTFYIKLSYNIFHDELGDELYEELAKERVLVN
                     LFIDTIRVHKGSIWCDDISTPAKESFNDIIARSLKDALDLLDDDFGSNPEKWKWGKAH
                     KLVLKHPLGEVKVLDWLFGYNRGPYPIGGSFHTVCPYSYKLNNPFVITDGASQRHIFP
                     MHNLNESLTVIPTGTSGHTASPYYCDQTLLYVGMKYHNDYIDSEIIQRNAKFIMTLVP
                     "
     gene            19132..19581
                     /locus_tag="PLV81_08330"
     CDS             19132..19581
                     /locus_tag="PLV81_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10146.1"
                     /translation="MQFETTTCITKVHRELLTYYAEIFNISVTDLITSLIHYAALCNK
                     RNPKTFKGIQYRKRYKDEWERIHLYVRYQEYEFLLDMKKMWKMSVALLIEYCIENVLE
                     EFVKRLLQEDDTYSYRFTNYTFKFEKIYGFQSYQIIWGLPPEIMEKT"
     gene            19710..>20080
                     /locus_tag="PLV81_08335"
     CDS             19710..>20080
                     /locus_tag="PLV81_08335"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOM10147.1"
                     /translation="MNNPELILKHKNIKLPAINQLGILVPDIHEAVTNYIRILGIGPW
                     FRSKTVKHEVLYCGKPITIDVDIVLAFHKGIEVELIALKGGEECIYTNLITTSGGGLH
                     HVGSLVLSYDQGMKRVHEAGI"
     assembly_gap    20081..20180
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20181..20947)
                     /locus_tag="PLV81_08340"
     CDS             complement(<20181..20947)
                     /locus_tag="PLV81_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOM10148.1"
                     /translation="MFFLKKLITSAMLPPGIFVIVFIICGVYAKRKSITILSLLCALL
                     LYALSIEPVKDVLFYHLEKQAHAIAPGIHVHKIKITNGTQNGLNPINTIDAIAILGGG
                     VYENGNFKEDSVNRLLAGYFLYTTTGKPIILSGGSAGGNIPESYTMYSVLQRLNVKKQ
                     DIIIESQSRDTDDNALNVIKLCERKNYRSIALVTSAYHMPRAYKLFGKSSVSVTPVLC
                     DSKLDMRYTMYSFLPQFSSFANSTKAVREYIALAVLC"
     gene            21347..22402
                     /locus_tag="PLV81_08345"
     CDS             21347..22402
                     /locus_tag="PLV81_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase family protein"
                     /protein_id="HOM10149.1"
                     /translation="MCGIVGIINTDRTKIDGSYIREAIRIQNERGNGLGGGFAAYGIY
                     PEHKDNYAVHIMYQGGRHSDIIKKIENFLEQKTKVHHSEEIPVYPTSIIPSGPYFKRY
                     FVNPLKEQIPVGKNEDDAMVDIVMSINRMGDAYVISSGKDMGVFKGVGFPEDIADFFR
                     IQDYKGYMWIAHNRFPTNTPGWWGGAHPFTILDKAVVHNGEITSYGTNKRWLEMFGYF
                     CMLQTDTEVISYIFDKLTRKDGLSVDDACFVMAPSLWEDIEHENAHKKMLLRQLYGQA
                     IVNGPFGIVLTHSGGAIVLNDRNKLRPVVCARVGSTYYASSEECSIRLLSPKVDELWS
                     PKGGEAVIVNLAKEEAC"
     gene            22411..23928
                     /locus_tag="PLV81_08350"
     CDS             22411..23928
                     /locus_tag="PLV81_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOM10150.1"
                     /translation="MKNNWKTIPPFHPEIDYSRCDYKNGCNICAHECSWGEIALKPEK
                     LDNGTVVYKPVANLTSCGSCQRCVKMCPYNAIHIVNSPMYTSHGYWTPDYVGNIWKQA
                     SSEGGVLLVGNGATGPQRRYFDHLMFDACQVTNPSIDPLREPMEIRTYLGRRPEKWGQ
                     DTYPLLKLEVPIMFGAMSFGALNLRVHEAEARATKEIGTYWNTGEGGFHKSLREKYGS
                     NTIVQVASGRFGVSEDYLKSAAAVEIKIGQGAKPGIGGHLPGEKVTKPISQTRMIPEG
                     SDALSPAPHHDIYSIEDLRQLIYAIKEATDYKVPVSVKIAAVHNVAAIVSGIARAGAD
                     IIAIDGFRGGTGATPLQIRQNTGIPIELAIAAADERLRQEGIRNQVSLVASGGIQCSS
                     DVLKAICLGADAVYVATPVLIALGCGMCQRCFTGKCAYGITTNKAGLAERIDVDEATW
                     ALKNLLHAWAEEIEELMGSMGINAIEAMRGNRDRLRGVGLNEEELRILGVKHAGA"
     gene            23943..26117
                     /locus_tag="PLV81_08355"
     CDS             23943..26117
                     /locus_tag="PLV81_08355"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOM10151.1"
                     /translation="MVLTATLNAKGVYYKMLNRQIRELVAKGETTVILENVLGQRYIG
                     GGLNADVTITINGTPGQDLGAFMNGPVIIVHGNAQDGIGNTMNAGTIVVHGKAGEIPG
                     HSMRGGQILIRDDVEYRAGIHMKEYETQIPLLVIGGTAKDYCGEYMAGGRVVVLNRFD
                     EKDPVGNDIGTGIHGGAIYIRGNVDKRRLGVGAILADMDNEDIAFLQNVLQTFKATFP
                     YVELSHITLNDFKKITKKGHRPFANLYAPAMNIKTKRPIHLNLTPPCTYACPSGIPTP
                     VFINLIKDGKIKEAQLLMDEYTPFRMSVCGTVCPAPCMQACSRNMIDGPMNIPYLARE
                     YYPDFNPTVYGKPKDKRIAIVGAGPAGLSAAWQLARRGYHITVFEKTADIGGKVRMAI
                     PKDRLPDEVLSKDLNRIKSLPIEFKVNTPVDKNLFAKIYKEFEAVLVATGAYVSRKLQ
                     VKGGERIISGLEFLIGINEGNPMDLTGKDVVIIGAGNVGMDIACESWRHGAKSVTAVD
                     IQKPLAFGKEKEMAERLGTKILWPRNIDYLDGSHVYFTNGEALPADVVFISIGEKPDL
                     SFLGDTVMVNERGAIVTAEKSFKSSDPKIFATGDIIKQGLITDAIGMGRLAAMEIHAT
                     LSDEDFIYPEKTLVPKRRINIAYFGGETREVDRCISCGTCIYCDKCVEACPQGAITRN
                     GEIFTIDAELCTACYTCVNVCPRGALQPGDFDAVVQDVLFKK"
BASE COUNT         8080 a         4412 c         5674 g         7759 t
ORIGIN      
        1 attatgggaa gtaacctgta ccacagtgtg aaatcaaaat gtaaaatata tatatcctgg
       61 tacaaaaata acgcaatgac aatagcaacg gctggagatg taattttgct tattgttaat
      121 gttttgtaac tttgattaat gtaatattgt tgttttaaat cttcagtttt catataccat
      181 tacatataat tccttgcata attatacaat atagtaatta catgatagtg tcaatacaaa
      241 catttcatgg ttaagtaaca taaagtttca cacgaaaaag agagatttta tatgcaagca
      301 atatcccgaa cctttcattt caattaatct cttgacaaaa atatgtatga ttgtattaca
      361 taatataaga ctgctatcat gaactatcgc caccattaaa caggaggtaa gtatgccgct
      421 taacgaaaaa gtcattggta aaaaatacaa atctgatcct gtaaaaatat cgcagcatga
      481 aagtatctat tatgcccttg cctataatga agacaatgat gcctattttg ataatcgaag
      541 acctggcggg attattgcac cgcccatgta tgcggttttc tacggggctg gtcccactgc
      601 tcagataatg tttgatgcag aaattggtat caacatgatg tttgtggtgc actatagcca
      661 gtactttgag tggataaaac ctgtgaagcc tgatgatgag gtcacttccg aaggaactat
      721 cacccatatt acagttaagg agaagggaag tattttgggc tgggagacag ttactaaaaa
      781 tcagcatggc gaagtagtgc tcaaggccaa atgggaattc tttgatagaa gtgctggttc
      841 cggaaaaccc gatgaaaagc ccgaaaccga aactaatcca tcgcagtttg tgtttgaaga
      901 cactatgaag gtaaaaaacg ggcagacgta tatctatgct gaaccatctg gtgatcacaa
      961 ccccatccat gttgatgatg agtttgcaaa aaaggttggt ttgcccggta tcattttaca
     1021 ggggctttgc accatggctt tttcacataa gatggcagtt gatcatctga cacctgatcg
     1081 cgatccgtta aaggtgcgag aattatatgt gcgatttgca cggccggtat tgccgggaca
     1141 aacgctaaca tttaaaggat acaccataga aaagcttgca gatagaacaa gatatggaat
     1201 agttgcgtta aataatgatg gcaaggatgt actgcgcaat gcgtggtgtt ccgttgccaa
     1261 ataaaaaata atgtttaaag caggagggta ttatggctat acgatttgat ggtaaagtag
     1321 ctgttattac tggagcaggt tctggtttgg gacgcaccta tgcgttagag tttgcaaaac
     1381 gtggtgccat ggtggttgtc aatgaccttg gcagttcacg agatggttca ggtgcaaaca
     1441 gcagtgctgc tgatgctgtt gtggaggaaa tcaaaaaagg tggtggcaag gctgtggcaa
     1501 attatgattc ggttgctaca gtgcagggtg gcgagaatat catcaaaact gctatggatg
     1561 catttggcag ggtggacatt gtggttaaca atgccgggat actgcgtgac aagacatttg
     1621 ttaaaatgac cgaggacgaa tgggatatag tgctggcagt gcatttgcgt ggtgctttct
     1681 gtgtgactgc tcccgcattt aaaatcatgc gcgagcaggg ctggggcagg attatcttca
     1741 ctgcatcagg ggcaggtctg tatggtaact ttggacagag caactatgct tcagcaaaga
     1801 tggcgcttgt tggtcttatg aatgcgctca agctggaagg agcaaaatat aatattttag
     1861 ttaacacgat agctccggta gcagcctcac gtatgaccga ggatgtactg ccaaaagaaa
     1921 tttttgaaaa gttgaaaccc gaatatgtta caccactggt aatgtactta tgttcagatg
     1981 aatgcaaaga tacaggaatg atattcaact gtggtggagg atggtatagc cgaactgcaa
     2041 tcatgtgtgc aagcggtact ataattcagg gaaagggtga gataactgct gaagatatca
     2101 tggcacagtg gagtaagatt accgatttga gtgatgccaa accattaaat aacattggcg
     2161 atacatttgc atacatggca ccattgttga aataaataac agcatggtag agatgccgcc
     2221 atgcaccggg aagatgccat gcgtggtatc ttcccggtaa aataatggtg ttgaaaaata
     2281 ttacgaggag gttcgtcaat gattggaata gtttcctatg gtgggtatat tcccttgcgg
     2341 cgattatccc gcatgcaggt tgtacagcag atggcatggt acaatccact gctctatatg
     2401 ctcatggcgg gagaacgttc cgtagccaac tgggatgagg attcagtaac aatggctgta
     2461 gcagcatcgt ttgatgcact gcatctggtt gatacaaaga gtatagatgc tgtgtatgct
     2521 gcttcaacaa catttccctt tgatgacagg cagaatgcag caataattgc cgaggcgttg
     2581 agtttgcagg atggtgtacg tactgttgat atgtcaggtt cactgaaggc aggagtaaca
     2641 gcaattgttg cagggcttga tgcaattgaa tctgattctg cacgcaaggt tttagtagct
     2701 tctggtgaaa ggcgttatac acgggctgct tctacgcttg aactattgta tggagatgga
     2761 gcagctgccc tggtgctggg gagagaacat gttattgctg aatttttagg gtcatgcgca
     2821 tatcatgaag attttattga ccactaccgt ggtgctgaaa aacggtttga ttatccatgg
     2881 gaggagcggt tcatacgtga tgaagggttt ggcagaatta tccccagagc aattaaagcc
     2941 tactgtgaaa aataccatac tgatatatca gaatttgcta ctgtaattta tccatgccca
     3001 tttgcgcggg aacatgctac aattgcaaaa aaaataaaat ctagtagtgt acacactaac
     3061 ctgcatgaag gtgtaggtga taccggtaca gcgcatcctc tgataatgct tgctcatgca
     3121 cttgatagcg caaaacccgg tgataagata atggtggttg gatttggcca gggttgtgaa
     3181 gtagttgcat ttcaggtaac cgatgcaatt gaaaacatta agcgtcctaa aggcattacc
     3241 gtaagcatgg cacggaaaaa agatataccg tacgcaaagt acttaaaatt cagggatttg
     3301 atagatgccg atataagtat acgtgctgaa gtaatgccaa atacatcgct atctgccctg
     3361 tggcgacaca ggaaaatgat tcatggtttt accggatatc tgtgtaaagc gtgtggtaca
     3421 ccacaattcc ccccatatcc ggtatgcgtt aatcctgcat gtaacaggtc cggacaaatg
     3481 gagaattaca gttttgctga caaaccgggg acagtgctca tgtatactgg tgatatgctg
     3541 gcagcttctg ttgacccacc tgctatctat ggcctggttg aatttgatgg tgggggcagg
     3601 acattgttgg atttcactga ttgtgagctt gataatctaa aagtaggttc acgggttgcc
     3661 atgtcatata gaatcaggcg agaggatact acacgaggct ttacaggata tttctggaaa
     3721 gctgtaccac taatggagta aggaggaatg tatggctacg ggaataaaag ataaagttgc
     3781 aattatagga atgggatgta ccaaatttgg tgaacgctgg gatgcagatg caaatgcact
     3841 tatggtggag gcatttaatg aagcgatagc tgatgcaggc attgacccca aggaaataca
     3901 ggcagcctgg cttgccacct gttttgatga agtcaatgtt ggtaaaagtg cattgtcagc
     3961 agcaattgca ttgcggcttc ctaacatcgc tgttacccgt gtggaaaatt tttgtgcaag
     4021 cggcaccgaa gctttcagag ctgcagtgta tgctgttgca tctggtgcct atgatatagt
     4081 actggcgttg ggatgtgaaa aacttaaaga tattggttat ggcggattgc cggaattcag
     4141 ccaggcaatt ggtattttaa atagcctgtg gtttcctaat ataactgcac cgggatgttt
     4201 tgccatgctt gcaggaggat actgtgcaaa gtatggatta aagatggaag acgtaaaaag
     4261 ggcaatggca catatatcgg ttaaaagcca tgcaaatggt gcacttaatc caaaagcaca
     4321 tctgcagcgc ccggtaactg aagagcaggt tttacaatca cccatgattg catatccttt
     4381 aggtcttttt gattgttgtg gtgtgagcga tggagcagca tgtgccatag tatgcagcgt
     4441 tgatgtggca aaggctttaa agaaaaagga ttttgttaca gtcaaggcct tgcagatttc
     4501 agtttcaaac ggtgaggaaa tggcattttc taactgggat ggtgcacatg tggtgaatac
     4561 cgaaaaagca gccatgagag catatcagga agctggtatt aaaaatccgc gtgaagagtt
     4621 gagtctgttg gaagtacacg attgcttctc aataaccgaa cttgtaacta tggaggatct
     4681 tcatgtatct gagcggggga aagccgtgaa cgatgttatg gatggttttt ttgacagcaa
     4741 cggcaaaata ccatgccaga ttgatggagg tctgaaatgt tttggtcatc ccattggtgc
     4801 gtcgggactc aggatggtat atgaaatcta cctgcaatta catgggaggg ctgacaaacg
     4861 ccagttgaaa gacccacggt taggcttaac ccataattta gggggcttcc ccatgatgaa
     4921 tgtggttagt gtggcgatag tgggccgcta caaacaataa tgttgttatg taaaaatatt
     4981 caggcttgac aaaatattct tgcttttact atatatgtat agtataggcg atagttacta
     5041 tcgcctaaat tttttataaa aactacaagt gaggatttca tgtttgaagg tgtttttaca
     5101 gcactggtaa cgccttttaa gaatggcaaa attgattacg attctctgga aaaaattatt
     5161 gagcatcaaa ttgcaggggg agttgatggc gttgttccta tgggaacaac cggcgaatct
     5221 ccaacagtca gttatgagga gcatgaagag tttatcattt gtgttgtgaa gatggtaaat
     5281 aaaaaaataa aagtaattgc tggcacaggt tccaactcaa ccgatgaagc aatctatctt
     5341 tcaaaggctg ctcaggatgc aggggttgat ggggtgttac ttgtcaatcc atactataat
     5401 aaaccaccac agcgcggtct tattgcgcat tttgaacagg tagcaaaatc catttcaata
     5461 ccggtcgtgt tatacaatat acccggaaga accggaataa actttttacc cgaaagtgtt
     5521 acagagcttg tgaagcgtgt acctaacatt gttgccatga aggaagcatc gggtgatatt
     5581 aatcaaatga tgcatctcat tgaattatgt ggtgatagga ttacacttct ttctggtgat
     5641 gataatctgc ttttgcctgt attaggtatt ggcgggaaag gtatcatttc tgtactttct
     5701 aatctgttgc ctgcagatgt aaaggctgtg gtaacgctat ttaaggctgg caaatttgaa
     5761 gaagcaagga agaagtttta tgcattattg ccactgtgtc gagcaatgtt ttatgaaaca
     5821 aatccaattc ctataaaagc tgccatggaa atggctgggt tctgttcttc cgaagtgcgg
     5881 ttgccactgg ttccactttc tgacgataat cgaaaaaaat taaaagatgc tttgcttgcc
     5941 tatggagtga aactatgaga attgcaatat gtggaattgc cggcaggatg ggcaaagcta
     6001 ttctaactgt tgtccttgaa aaaggtcata cgctttcaca ggcacttgat gcagaacaat
     6061 gtccgctttt aggccaggat gcaggtagtt tgatttcaaa gaaacttgat gttagcattg
     6121 agtcacttcg tagtgcaaag cttactgctg atgcagtgat agatttttct gcacctgctg
     6181 ctactgaggt gttgttacag aaagcagtgg aatataaaat tcccttagtg atagggacca
     6241 ccggattttc tgatgcacaa aaacaaaaga tagaacaagc ggcacaaaca attcccattg
     6301 ttttttcccc caatatgtcg gttggggtta atctgttatt taagctaaca cagatggctg
     6361 ctttggtgtt acaggatggc tatgatgttg aaatatttga ggcacatcac cgtttaaaaa
     6421 aagattcacc ttcaggcact gcaaaaaagc tcattgatgt tgtcatggaa acacggaaag
     6481 atttgcacaa tgcaaagctt atgcatggaa gggaaggcat cattggccag cgtactaata
     6541 atgagattgg tgtcatggta atgcgtggtg gtgatattgt tggtgaacat actgtgtact
     6601 ttattgctaa tggagagcgg atagagctaa ctcaccgtgc tacaagccgt gaaaattttg
     6661 cacgtggtgc tgtaagggca gcagagtttt tggcagataa aaagaacggg ctttatacca
     6721 tgtttgatgt attgggatta taatagcaat gagtcagaat gatgtgaaaa acgatactgt
     6781 atcatttgaa gaaaatcctt actcgcctga gataaagaaa cccgaagata ttgtcaggct
     6841 attctatatc cataatgtcc caattttgcc ggttatatca aaacgtggaa tattatttgg
     6901 aatattacga aaagatgatg ttattgctga actcagtgat attgctcgca gcacagttaa
     6961 aattgatgag tttgttcaga agctggcaaa aaagatgacc atggatgaac tcttgccgct
     7021 ggtagtaaat gttaaggatc ttattgccat agacctgttt ggtgaggttc agggtcgcat
     7081 gacacgactg gatttattta tggcagctga gggacactat gaaaaaaagg aaaaagaaaa
     7141 agggaaggaa gaaaaagaaa cacaacccaa agatacagtt attgaatggc tcatctatat
     7201 ggttctggaa catataccca gagcacttta tgcggtaaac agtaatggaa aaacaatatt
     7261 ctttaacggc cattttgaag agatgtatat caatactttc cccgaatcaa gcgatgttga
     7321 cattgcattt actgaaaaaa tactgatgga ttcagctatc aatgatatat acacatcgta
     7381 tgtatataag cggcagtttt tttataataa agaattgaaa gcatattatg aacgggtacc
     7441 gttaaaaagt gaaaataaaa cagcaggcta ccttttcatg tttgaaaggg atatcacaaa
     7501 gcaggatttg cttgtgcctg atatcatttc tgacactgat ggtattgatg agcttctttc
     7561 gcgtgctgag cgttctattc tggtacaggc gctaacacaa tcaggttttg atgtagcaca
     7621 ggcttcaaag atgcttcata ttactgttgc tgatttaaaa aaaagaataa aaaaatatga
     7681 catcacagtt ataaaaaaag aaggttaatc tattacatgg cgcatacttc attaaataac
     7741 acaattaatg tattacttat tgaagacctc aactatgacg cacaattgga acaaactatt
     7801 ttagaaaaat caaattctaa gtattccatt caatgggtgt caaataaaga agaattttat
     7861 aaagcctgtg aacaatgtat acctgatgtt gttgtaagtg attatcatct cccagatata
     7921 acagctgaag aaataataga atatataaat gccaatttca aatacgtacc agttatcatt
     7981 atcagtggtg ctataggtga agagaccacc gtagaaatta taaaaaatgg tgctgttgat
     8041 gttctactaa aacagcattt attcaagctt cctcaggcta tagaacgagc aatagttgaa
     8101 acgtcaaagc gtaaggaatt acaagaagct ctggaaaaat taaaaaagac aaaggaactg
     8161 caagaattaa ttttgcagaa tgttccagtt ggcataattg ttgcaacaaa agatggtaca
     8221 gttattgaaa ttaacaggca tgctcttggt atgcttggat atgataccgg taacacgcct
     8281 cagcattttt cagctcaagg tttttttgtt gatagttccc agcgaaatca tttttttaag
     8341 gttcttaatg caaaaaagat aatccaaaac tatgaagcaa gggtgtatcg taaggatggc
     8401 cagataagga tttgttcttt ttatgctgtg atgcataaga ttgacggcca gcaatacata
     8461 ttcacagcaa ttattgatgt gaccgagcag aaagcactgg aagacagagt attattttta
     8521 tctttttttg atccacttac acaattaccc aacagagcac ttttaaaaga tcgcatagat
     8581 ttagcgttaa tgcgtgcacg gcataataaa cggtatgttg ccgttataac acttgatgtt
     8641 gaccgcttta agtatataaa tgattcgttg gggcataaag ttggtgatga tattttgctt
     8701 gaaatatcgc aaaaattatc aaaatcacta cgtgacggtg atgtggttgc ccgcataggg
     8761 aatgatgaat ttggtatcat gcttatagat gttgctcgta atgaagatat tatttctgta
     8821 attaataaaa tacggaatat atttacactg ccatttcatg ttgatactaa tgagttatta
     8881 atcactgcaa gtatgggagt atcgatattt cctactgatg gtaatactgc tgaagaatta
     8941 ttgcaaaatg cagatatagc acttgctaaa gcccgagagc agggtcgttt tacctgtgaa
     9001 ttttatacaa aggatttaaa taaaaaggta tctgattata tcagcttgga ggccaaactt
     9061 aaaagagcta tcgctcaaaa tgaatttgaa ctgtattatc aaccatactt caatattaaa
     9121 accagaaaag tggcaggtat ggaagcgctg ctcaggtgga atagtgaagg caacgttata
     9181 tcccctgcaa cgtttattcc agtacttgag gatagtggtc ttatcattga tgccgggaag
     9241 tggattattg aagaggtttg caggcaactt ttagcgtgga agcaagcagg gttaccggtt
     9301 aagccagtat cactgaatgt atcgccaatc cagttccagc agacagactt taaagatatg
     9361 gtaatggata ttatttcgtc ttatgcaatg gatacgggat taatcgtgct ggaaattact
     9421 gaaagcacct atatgcgcaa tattgattat acaaaaaaag tcatgcaggt attgaaggat
     9481 gcaggattgc attttgctat tgatgatttt ggaacaggtt attcgtccct gagttctctt
     9541 ttagctctat catttgatta tttgaaagtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnna tgaataagaa ttattctgtt aaagcattca atgcttcaga tttatataca
     9721 attgaagatg tctatgatgt aaaagatttt aaaaaagttg catatatgca gcctttgaaa
     9781 aatgacaaca tggtgtcatt tgaaaaggca gttgaaaata tctataaact gcatatatac
     9841 aactacgaat tgagcaaggc cgaagccctc actgacatgc gcacaaaata tggcgttcag
     9901 tatctcatat taatggagct taaagattgg cagaaagtat cctgggcacg tgctattgat
     9961 ttaactaaca tggaagttgt gtgggttgaa aattaccctg cccagtatag agatactctt
    10021 gaaacagttg ttgatcattt tattgccagc atgagtaagt aaaaatgtta taatcatgaa
    10081 aaaatgcttt atcatagtat tgatgtatgt gctgatgctt tctggcacag tatatgccag
    10141 gacaacagtt gcatttggat atttaacaaa cagaagcggc aatccaaatt tcaattatat
    10201 ggaggtcata ttccccaatt cttttgcatc ctctattgaa gcggtgtatg attgtactgt
    10261 cataaagccc cttaaattaa atgaacttct tgaaaaaaag caattaacat tacaaagaca
    10321 ttatgaaaat catgaattac cgcaaataac aaaagctata ggctgcgacc tttttatctt
    10381 tggttatttt ataccattac ctgataatac catcaaaatt gttttaacat tgtacgagag
    10441 tggttccaac cagatattta cgtttaccaa cataggcaaa atggaaacag aaattttcaa
    10501 aatcattgat agaattaccc tggtggtgtt tgatttcttt ggaaagaatg gtttttttat
    10561 gtcaaagcct attatgcgcg gcagttcggt tgggctgatt acaaacctga atcctgaaga
    10621 gctcaatagt atgtatgctg aattttttaa tgcagggtat tctgttagtg cagtgcaggg
    10681 gaatgagctg gatagctact atgctcatga aagtgaaatt atgaattatt ttcagtatat
    10741 aagcactgat gccaactcat atgatatcat tactgattgg agtaaattta ccatgactat
    10801 ggggacatgg gagggcagga aaaagaaaga atggcaggaa gatctaagga aaatatatga
    10861 aatatatgac ttacattata aagtgtttaa aaatacagta cttgaaagat acaaaaatat
    10921 atatccagga tgtgattatt tactgtttgt tgtgtataat ccttcgcgta caagctgctg
    10981 ggcgcgggct attgacataa aaacaaaaga attgatattt atgcactcaa atattgaagc
    11041 atctgaaatg aatgccaatc tccagaaaac cacacgtcag cttatcacga ttattcagac
    11101 accaacagca ctgcctcaaa aatcaaagaa gaaataagac acannnnnnn nnnnnnnnnn
    11161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11221 nnnnnnnnnn nnnnnnnnnn nnnctgagtg tgattgctac aggaaactgc tgaataaaaa
    11281 atacatattc gccacccagg aaaccctgaa tttgcatata ttcagtttca ttaatggtgt
    11341 ttgggtcttc cgtagtagat gaatatgttt gtctgaagaa tatatttatg tcatctctaa
    11401 tacttgttgc gattttcagc ccaaagccgc tctgcgactt tgcgtcacct gtattattga
    11461 tcatgagcaa tccatcaaca tatacattag ccattgctat tgttgtactg gaaagcacgg
    11521 taattatcat aacaattatt tttttcacag taacctccat tgaatcatgg ctcctattac
    11581 atcaaaagtg cttacatagt aactatcgcc caattttaat tgtttctaag tatatactta
    11641 tgaatatgtt tatgtcaata taaaaaaatt gaaattctgc aattctttta atgtatggtg
    11701 atttcattgg taaaatataa tttgtttata ctataaaaaa gacttgcagt agtacaatgt
    11761 ttgagaataa tttaataaaa cactctatat gttcgaaatt ataaatatag cttacagagg
    11821 acaaggtttg ggttttaatg aaaaacagaa tactgggcat attgttttgt tttatttgca
    11881 ttcacgctgt ttcggttgca tttgctgagc agcctgatag tatcactgca attataaatg
    11941 aagagcaaaa taaagaacat tcagatgcat tgctgcagat gctgcagttt atagatagtt
    12001 tatatgataa tctgcaaagc cgggtgaaga atggtgaaaa gatagtaata ttttttgacc
    12061 ccgcacatgg tatagatgat gatggcaaat gggaaggcga aaaaacagga aggctaagct
    12121 gtacagggtt acctgaggaa tattattctc ttgcactttc acgccagcta tacagtcttt
    12181 taaagaaaaa tccatatatt caggtggtaa gcacacagga ttatattgat gcccttgaag
    12241 gaaagactga tacgtataac aacatcacct tcagggaaac agtggaactg gcaaaaaatg
    12301 ccagggctgt acttgttatt tctgaacatc ttaataatac agcatcaata ttcaaagcgt
    12361 atgggcttgc aaatgtaaaa ggcatacata ttacgtacag taacgggaaa gcctacctta
    12421 ctcatatacg ggaagagcat aaagggtttt taacgctgta taataagttt gatgccagtg
    12481 gtttttcgta cagggttgca agcaacgtta aggaaaaact tttggcttat aatttgcgtg
    12541 ttaacagctg gaattatggc accgtagcgg atgaccggtt tacgtatttt gtcaattacc
    12601 ccatatctgt tatctttgaa tccggtttta tttcaaaccc ggatgatgaa aaaattttaa
    12661 gtaaccctga gtatcaaaaa ctcattgcac aagcacatta tgatgcaatt cttgaaagca
    12721 ttgctaatgt ttttggcatt gatatttctg gtttctgggg accacggatt gttgacactg
    12781 ctgatccatc acggaatttg ctgttactta aattatcccg aattgcctgt tattacattt
    12841 cccataatga tactgccaaa ggtgtgcatg tattgtcgtt gttactgaaa gagtgtgatc
    12901 cagcaaagga tgctccaatt atagaaaata tacaggacat gaaataccgt attgaacgtt
    12961 caaatgagtt gtatactaaa gctttagctc tgaaaagaaa gggtaaaatg aaacgtgcac
    13021 ggtatactat gcgaaaagcg ataagcctga taagcagatc acctgttttt tatgaattgc
    13081 gcaaagcata tcgtgctgaa tataaagggt taaaagaagt gattgaacaa aaatcaactg
    13141 cgcccaggtt taaagctcat gatacaatgg ttaatccttt agcttctagc attaccaaac
    13201 cagtgctggt ggtgattgaa aaaaatatga ctccccagga agctatattc aatgctttgc
    13261 agtgtggtga agtactgttg aaaaaaattt caagtcagtt tgtacaaaca aagattaagg
    13321 ttgggaaaaa aagatttgaa aaacccacac aagaaggtat atacatagta cagcttacag
    13381 caaaaggcaa tgttaaaagt gttaagcgtg ttgcaattgc aaagcttgac ccatataagt
    13441 atcaaaatca aatgtattta aaaaactcgt attgtgtgcc aaaagaaaga aataaatatt
    13501 tgtaagcatg gcaactggta tggtaagcct taccacttct ttggtgatta tctatttcat
    13561 acatttaatg caaaaatcct gaaactttct attaatgcac atttgggctg tccaaatcgt
    13621 gatggtaccg ttggcagtgg aggatgcatt ttttgctctg aaggatcggc atcaccaact
    13681 gcaacagggt ccacatcaat tcatcagcag atgcaggctg ctgttgatgg atttaatcgt
    13741 ggtatgtata tcccgcgata tattgcatat tttcaagctt ttaccaatac gtatgctaca
    13801 gttgatgttt taaaaaagct ctatgatact gctttagcat ttcccaatgt tgtggggctt
    13861 atgattggca ctcgccctga ttgtctgccc aatgatgtgc tggatttgat agccagctat
    13921 aaaaaagaaa attttgagtt atggcttgag attggcatgc agagcatgca tgataaaagt
    13981 ttacaatttt tacagcgtgg tcacactcat gctcaaacag tggatgcaat cacaagagca
    14041 tcacagcgtg gcatcccggt gtgtgttcat gttatactgg gcatacctgg tgagagctgg
    14101 gatgatatga tggcaacagc aactgaaatt agcaggctaa aggtacaggg agttaagata
    14161 catcatcttc atgttataaa aaatactcca cttgaagtat tgtatatgag cggcaatgtc
    14221 ccattattat catttaaaca gtatgtatca tatgtgtgtg attttattga acgtctgcca
    14281 ggtgatatca tcattcaccg cctgcttggt gatcagccca aagatatgtt aattgccccg
    14341 gcatggggat tgcacaaggg gacggtgctg aaagccattg aagatgaatt gctgcggcgt
    14401 ggtacctatc aggggttttt gtgcgatagt aactgataat tttatggttg ctttttaact
    14461 gaagcttgtt gtattcaata tttaaatttt attcactatg ggagtaaaca ctatgaatct
    14521 gcgtcctgac acctatgctc gttcaacgct ggaacggtat actggttctt caattgatga
    14581 ttttggtgaa tatatcattt tatgcaattt tcagcggtat gttgatgatt ttgcatccat
    14641 gacaggtgca aaagtcagtg ctggctactg gagctgtgca catagtcgtg aacgcaacat
    14701 atccattata aattttggcg ttggttcacc atcggcaggc atcgttgttc attgtctgtc
    14761 atatctggat aaaatacaga cagtgctcat gctgggaatg tgtggtggta ttgatgataa
    14821 cctgcaggtt ggcgattttt taatccccac agctagtgta cgggatgaag gcacaagtat
    14881 tcattatata ccaaaagatg ttcctgcaca gccaagtttc tggattaaca gaatctgtga
    14941 aaaggttata acaaaagaaa caggtgtggc acccaaaact ggcataatgc ttaccaccga
    15001 ttaccgcatg tgggaatttg ataatgaatt tattgattat atcttaaaac acaggatttt
    15061 agcaatagat atggagatag ctaccttatt tgcagtagca tatgcaatga atgttcctat
    15121 tggagctatc atgctcattt ctgatctgcc attaaaaaaa ggtggcatca aaagcaaaga
    15181 gagcgcaagt ttaatattca atgcttttac ccagaaacat ttacaattgg gtataaaagt
    15241 aattgagaat attcaggaaa aaagtggtga aggtattacc agattacgaa gtgagtggta
    15301 attaatttct tgactgatgt aaaattattg tattatattt tttaccatga ttaaaagaaa
    15361 tatttttttc attacttttt tcttatttat ctctggatgt gctagtacat ctatacctgt
    15421 tacaaaaaca tatgaagatg ctgcaaatgt tttagccaat caattaaagc cggagctgta
    15481 taatttatct gaacacaaaa atataaagat cgccatattg ccatttggtg gtcccaaagg
    15541 caaacctaca ccttttggta aaaaaatggc aacattattg caatcaaagt tgttttcgca
    15601 aaactggaat ttaattgaac gtgatcaaat agataaacta attaacgaaa aaaatctatc
    15661 tgcaactggt attgtcgaag ataaagatta tatgagtaca ggatcaatag caggagctga
    15721 tttcattatt gtgggtacta catattatgg gaaaaaggca ttaattacag tcaagattat
    15781 aaatgtacat actggttcaa ttgaaggtat tgcacaggta tatcttacgt tttcaaaatg
    15841 aaaataagga ggttttatgc cagcattgaa aattacagcc aatggcaatc ccgtgactct
    15901 tgaaggccag atgccatcag ttggtgataa agctctggat tttacagtac ttgatattaa
    15961 ttttgcacct gtttctctgc taaactttaa aggtaacatt attataatca gcgctgtgcc
    16021 ttcacttgat acctcagtgt gtcagttgca gacaacaaga ttcaatcagg aagcagcttc
    16081 attggatgct gtggttctga ccataagcat ggatttgcca tttgcccaga aacgcttttg
    16141 tgaatctttc aatatacata atattaaaac attatccgac tttaaagata aaagtttcag
    16201 tttaagttat ggcatttata tacgagaatt gggattaatt gcccgttcag tgtggataat
    16261 tgacaaagca ggtaccattg catatcgtca gattgttcct gatatcactc aggaaccaga
    16321 ttatgatgat gtattgaaag ctgccagagc gttgggtgca taaattaatt tggaaataaa
    16381 aggggctata tgaagagccc ctttttattg tttgtttatg gcaccaacgt cattatgaat
    16441 tttgcattgc gctgtattat ttcactgtca atatagtcgt tgtggtactt catgccaaca
    16501 tacaacagtg tttgatcgca atagtatggg ctggcagtgt ggccactggt accagtggga
    16561 ataaccgtta atgattcatt aagattgtgc atggggaata tgtgtcgctg tgatgcacca
    16621 tcagttatta caaatggatt gttaagttta taggaatatg ggcacacggt atggaaactt
    16681 ccaccaatgg ggtatgggcc gcgattatat ccaaacaacc agtctaaaac ctttacttcg
    16741 cccagcgggt gttttaatac cagtttgtga gcctttcccc atttccattt ttcaggatta
    16801 ctgccaaaat catcatcaag caaatctagc gcatctttaa gtgacctggc aatgatatca
    16861 ttgaaagatt cttttgctgg tgttgatata tcgtcacacc atatagaacc cttatgtacg
    16921 cgtatggtat caataaaaag atttaccaga accctttctt ttgcaagttc ttcgtaaagc
    16981 tcatcgccta actcatcatg gaagatatta tatgaaagct taatgtaaaa tgtatcaaat
    17041 agtgtggcag cactactgtc aacagtataa ctaccatccc aatctttcag tattgtgtaa
    17101 atatctttca acgattcttt cagtgaacta tcgccctcta ttatttgtaa cagtaatggt
    17161 ttccatatga tagccagttc tgatacctga tcggtttgaa ttgctttgaa atcattaact
    17221 gataatttga ctttttgctg taacagtttg gtgatgcgat tatagcgcca tggcatctca
    17281 aaccagtagc tgatataata aggatactgt tccatcactg ttttattgtt tgctgatgca
    17341 acaaatcctt ctttggggtt gtacacatgt ggctgctgct caaacggaac aatgcctttc
    17401 cagtcatacg cgtctgtcca tccaggattg accatcatgg gatttccttt tcgtatggga
    17461 atgccagcag cacagtacaa acctatgttg cctgaagtat cagcatacac aatattctgg
    17521 cttaccgagc ggaagtgacg aatggcactg gtaaaatcgt tccagttttt tgcgcggttg
    17581 agcatataca ctgtcagtaa ttcattgctt ggctcattgc ccatccagcg catggatatc
    17641 acatcattcg ttttcttgaa ttccgtaacc acagggccac ggtgtgtgaa aagaatttct
    17701 tttgaaacag tttcaccacc tttgattttt atgtcttctt ttactacagt gaagtttttc
    17761 cattgcccgt taaactggta ttgcattttg ttattggggt ttaccttctc taagtaaaaa
    17821 tccatatcat caaccataac gttggtcatc ccccatgcta tatgttcatt atgcccacat
    17881 acaatgcttg gttccccagg tattgcaacg ccggtaacat tgagtttacc ttcaaccacc
    17941 tgatgcatct ggtaccagat accaggtatg aacaaaccta agtgcatgtc gttagctaat
    18001 attggcttgc cggtaatact ttttttgcct gctaccgccc agttattgct tgcggaaaaa
    18061 atgccggcac ctatctcaat gagcttttgt gatgcgttaa ggattgtgtt cgatagctcc
    18121 ggtaagtgcc ttgccttagc aaatgatggg tagatgacat cagtgatata cttaacatcc
    18181 ggtgttaaag cttttgcatg aacctggtca acttttttcc atattttata aagtactgtt
    18241 tcagtagtcc atggcatggt aagatcccaa gccatatacc caattaaatt gaccgaatct
    18301 tcaaccttcc agttttcagg cttatatcct aaaatcttga attcaagtgg aaggttctct
    18361 gcatgaagtt ctaagtactg attcacgcca tcagcaaatg cttgcagatg agaaatgacc
    18421 gttggatctg tatgggcaat tacatagttt gatttttttg taatctgcaa agcgcgcatt
    18481 aaaacatcag tatccaccag atctttgcca aaaatctcgg ataatctacc tgtggtgaca
    18541 cggcggagta aatccatctg ccacatcctg tcctgtgcca tgacaaagcc agttgccagg
    18601 tacaggtctt tttcattttt tgcaaagatg tgtggtatgc catattcatc acggtacacg
    18661 tttacagctt gagacaaacc tttaagctgt agtttacctg aatactgggg cagaccagat
    18721 tttgccagat tattaacata taaaaataca gcaactgcca gaaccagtat caggataatt
    18781 aaaccaatga ttactttttt ccccctgttc attgttaaac ctccataacg tatttatgtt
    18841 attatataac aatgcatcat aaaaagtaaa gaaattattc caaaagattg taatgctaaa
    18901 tgttaaatat acaatataaa attattttat ttgacataat atttggatat gatatagatt
    18961 cagtaattgt ttcaaaatgc atattcatct agtttttata aaaaatgaaa tgcatttccc
    19021 agctaagata ataagcgata aaccttgttt tagaaaaaat atttttaaaa ggtatttact
    19081 aaactataag aagtaatact aaataaataa aatactttgg gaggataata tatgcagttt
    19141 gaaacaacaa catgcatcac aaaagtacat cgtgaactgt taacgtacta tgctgagata
    19201 ttcaacattt cagtaactga tttgattacg tcattaatac attatgctgc attatgtaac
    19261 aagcgaaatc ctaaaacttt taaaggcata caatatagaa aacgatacaa ggatgaatgg
    19321 gaaagaattc acctatatgt acgataccag gaatatgaat ttttgctgga tatgaagaag
    19381 atgtggaaga tgagcgtggc gcttttaatt gagtattgta tagaaaacgt attggaagaa
    19441 tttgtaaaaa ggctattaca ggaagatgat acatatagtt atcggtttac aaattataca
    19501 tttaagtttg aaaagatata tggattccag agttatcaaa taatatgggg attgccacca
    19561 gagataatgg aaaaaacata agctcaatcc catttatttg agataattat agtagcataa
    19621 tacaaccgat aaaattctgc attgtagcag agtaagacag tgcatggtaa tatacgttga
    19681 tgcatgacat ttttttattg gaggcccaca tgaataatcc tgaattaatc ctcaaacaca
    19741 aaaatattaa acttccagct atcaatcaat taggaattct ggttccagat atacatgaag
    19801 ctgtcacaaa ctatatccgt atattaggaa ttggcccctg gttcaggtca aagaccgtaa
    19861 aacatgaagt tttatactgt ggtaaaccga taactattga tgttgatatc gttcttgcat
    19921 tccataaagg gattgaggtt gagcttatag cccttaaagg cggcgaagag tgcatataca
    19981 caaatctcat taccacatca ggtggaggtt tgcatcacgt tggatcgctg gtgctgtcgt
    20041 atgatcaggg gatgaaacgg gtccatgagg caggtattga nnnnnnnnnn nnnnnnnnnn
    20101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20161 nnnnnnnnnn nnnnnnnnnn aaacataaca ctgcaagtgc aatatattcc cgcaccgctt
    20221 tcgttgaatt ggcaaaactt gaaaattgcg gcaaaaagct gtacatggta taccgcatat
    20281 caagtttgga atcacacaat accggtgtaa ctgatactga tgatttgcca aaaagtttat
    20341 aggcacgtgg catgtgatac gctgatgtta ccagtgcaat tgatctataa ttttttcttt
    20401 cacataattt aataacattt aaagcattat catcagtatc tctgctctgg ctttcaatga
    20461 taatatcctg cttttttaca ttcaatcgct gtagtacaga gtacatagta tagctttccg
    20521 gtatgttacc acctgctgaa cctcccgata gtatgattgg ttttcctgta gtggtataca
    20581 gaaaataacc agccagtagc ctgtttactg aatcttcttt gaaatttcca ttttcataca
    20641 ctccaccacc caggatagca atagcatcta tagtgtttat aggatttaac ccattttggg
    20701 tgccgttggt gatcttaatc ttgtggacat ggataccggg ggctatcgca tgagcctgtt
    20761 tttcaagatg gtagaataga acatctttga caggttcaat tgatagtgca taaagtagca
    20821 gcgcacatag cagcgacagt atggtaatgc tttttcgttt tgcatacaca ccgcagataa
    20881 taaacacaat aacaaaaata cctggtggca gcattgcact ggtgataagc ttctttaaaa
    20941 aaaacatggt cactctcccg gttactatcg cccaaaatta ttacacattc ttttgatgta
    21001 aagcactata ctgcaaaata ttttgtgatt ttataaaaat tattatggtt atacctatgt
    21061 ataatcttga ttagtttgct ttttattatt attttgtgat aacatcccat ggtatttttt
    21121 aaagacaaaa tagctctgta gattaagtag tatgtttttt aatggggatt ttaaaaaggg
    21181 taatacttta tgttaataaa cataactata aaatgaataa aaaaattcat ttgaatatgc
    21241 tcctaaaatt taacatggct gtaacataac tgcagccttt tgtattgaat tacgggatgc
    21301 gtgcgctaca tatggttctt accactaaac aattaaagag ggtgttatgt gtggaatagt
    21361 aggaataata aacactgaca gaacaaagat tgatggcagt tacatacgtg aagcaatccg
    21421 tatacaaaat gaacgaggaa acggccttgg tggaggtttt gccgcctatg gcatttatcc
    21481 agaacataaa gataattatg ctgttcatat catgtatcag ggaggaaggc acagcgacat
    21541 tataaaaaaa attgaaaact ttttggaaca aaaaaccaag gttcatcact ctgaagaaat
    21601 tccagtatac ccaacaagca taattccttc agggccatac tttaaacgct actttgttaa
    21661 tccgttgaag gaacagatcc ctgtgggtaa gaatgaagat gatgcaatgg ttgatattgt
    21721 aatgtccatt aacaggatgg gtgatgccta tgttatatcc agtggaaagg atatgggtgt
    21781 gtttaaaggt gtgggcttcc ctgaagatat tgctgatttt tttcgtattc aggattacaa
    21841 ggggtatatg tggattgccc ataaccggtt tcctaccaat actcccggat ggtggggtgg
    21901 tgctcacccg tttaccattc ttgacaaagc tgttgttcac aatggcgaaa taaccagtta
    21961 tggtaccaat aaacgctggt tagaaatgtt tggttatttc tgcatgctcc agacagatac
    22021 cgaagtaata agctacatct ttgacaagtt aacacgcaag gatggcctta gtgttgatga
    22081 tgcctgtttt gtgatggctc cttcattgtg ggaagacatt gaacatgaaa atgcacataa
    22141 aaaaatgctt cttaggcaat tatacggtca ggccattgtt aatgggcctt ttggaattgt
    22201 attaacacat tctggtgggg cgatagttct taatgatcga aacaaactgc gaccagtggt
    22261 atgtgcgcgt gttggttcaa cctattatgc gtcatcagag gaatgcagta tacggttact
    22321 atctcccaaa gttgatgaac tgtggtcgcc caaaggtggt gaagctgtta ttgttaattt
    22381 agcaaaagag gaggcttgtt aatggcatcg atgaaaaata actggaaaac catacctcct
    22441 ttccaccctg agattgatta ttcacggtgt gactataaaa atggatgtaa tatatgtgcc
    22501 catgaatgct catggggcga gattgcatta aaaccggaaa agttagataa tggtacggtg
    22561 gtgtacaagc ctgtagcaaa ccttacatcg tgtggttctt gccagagatg tgttaagatg
    22621 tgtccgtata atgcaattca tattgtcaac agtcccatgt acaccagcca tggttactgg
    22681 actcccgatt atgtgggcaa tatttggaaa caggcttcat ccgaaggtgg tgtgctgtta
    22741 gtgggcaatg gtgcaacagg tccccagcgg cgctattttg accacctgat gtttgatgcc
    22801 tgccaggtta ccaatccttc cattgaccca ttgcgtgaac caatggaaat ccgcacgtat
    22861 ttagggagac gccctgaaaa gtgggggcag gatacatacc cgcttcttaa attagaagtt
    22921 cccataatgt ttggtgcaat gagttttggt gcattgaatc tgcgggtaca cgaggctgaa
    22981 gcaagagcaa caaaagagat tggtacatac tggaataccg gtgagggtgg atttcataag
    23041 tcgttacggg aaaagtatgg ttccaataca atagtacagg ttgcatctgg ccgttttggt
    23101 gtaagtgaag attatttgaa gtcagcagct gcggttgaaa ttaaaatagg gcagggtgca
    23161 aaacctggaa ttggtggtca tctgcctggt gaaaaggtta caaagccaat ttcccaaaca
    23221 cgcatgattc ctgagggaag cgatgcgcta tcgccagcac cccatcatga tatttattca
    23281 atagaagact tgcggcagtt aatttatgca ataaaggaag caacggatta taaagtaccg
    23341 gtaagcgtca agattgctgc agtacacaat gttgccgcta ttgtaagtgg tattgcacgc
    23401 gcaggtgcag atattattgc cattgatgga ttcagaggag gtaccggtgc tacaccccta
    23461 caaattcgtc aaaataccgg cattcctatt gaactggcaa tagctgcagc agatgaacga
    23521 ctgcgccagg aagggatacg caatcaggtg agcctggttg caagtggtgg cattcaatgt
    23581 tcttccgatg tgttaaaggc aatatgtctg ggtgctgatg cggtatatgt tgcaacgcca
    23641 gtgcttattg cattaggatg tggtatgtgc cagaggtgtt ttaccggcaa gtgtgcctat
    23701 ggcataacaa caaacaaagc agggcttgca gaacgtattg atgttgatga ggcaacatgg
    23761 gcactgaaga atttgcttca tgcatgggcc gaggaaatag aggaactcat gggttcaatg
    23821 ggtatcaatg ctattgaggc aatgcgtggt aacagggaca ggctgcgtgg tgttggcctg
    23881 aatgaagaag aattaagaat tttaggtgtt aagcatgcag gagcgtaaag gaggatgtaa
    23941 caatggtgtt gactgcaacc ttgaatgcta aaggcgttta ttataaaatg ttaaaccgcc
    24001 agatacggga gcttgttgca aaaggggaaa ctactgtaat actggaaaat gttttgggtc
    24061 agcgttatat tggcggaggt cttaacgctg acgtaaccat aaccattaat ggtacacccg
    24121 ggcaggatct gggtgcgttt atgaatggtc ctgttatcat tgttcatggc aatgcacagg
    24181 atgggatagg aaatacaatg aatgctggta caattgtagt acatggaaaa gcaggtgaaa
    24241 tcccgggaca ttcaatgcgt ggtggtcaaa ttttaattcg cgatgatgtt gaatatcgcg
    24301 caggcattca catgaaggaa tatgaaacac agatacccct tcttgttata ggtggcactg
    24361 ccaaggatta ctgtggtgaa tatatggcag gaggccgtgt tgttgtactg aatcgttttg
    24421 atgaaaagga tccggttggc aatgacattg gtactggcat acatggaggt gcaatataca
    24481 ttcgtgggaa tgtagataaa cgtcgattag gagttggtgc tattctggca gacatggata
    24541 atgaagatat tgcttttttg cagaatgtgt tacaaacttt taaagcaacg ttcccgtatg
    24601 ttgaactatc gcacataacg ttaaatgatt ttaaaaaaat taccaaaaaa gggcatagac
    24661 cttttgctaa cctgtatgcc cctgcaatga atattaaaac caaacgccct atccacctga
    24721 acctgacacc accgtgtacg tatgcttgtc ccagtggtat tccaacaccg gtatttatta
    24781 acttaatcaa agatggaaaa attaaagaag cacaattgct tatggatgag tacactccgt
    24841 ttagaatgtc agtgtgtggg acggtgtgcc ctgcaccctg tatgcaggcc tgtagtcgca
    24901 acatgattga tgggcccatg aatattccgt atttagcgcg tgagtactat cctgatttta
    24961 atccaacggt atatggcaaa ccaaaggata aacgcatagc tatcgttggt gcaggacctg
    25021 ctggtttgtc tgctgcatgg cagcttgcgc gcagagggta tcacattaca gtgtttgaaa
    25081 agacggctga tattggcgga aaggttcgca tggctattcc gaaagacaga ttgcccgatg
    25141 aagttttgag caaggatttg aatagaatta aaagtttgcc cattgagttt aaggtaaata
    25201 caccagttga caaaaatctt tttgcaaaga tttacaaaga atttgaagct gtgctggtag
    25261 caactggtgc gtatgtttcg cgtaagcttc aggtcaaagg cggtgaacga attatttcgg
    25321 gtttagaatt tttgattggc atcaatgaag gcaatccaat ggacctgacg ggtaaagatg
    25381 ttgtcatcat tggtgcaggt aatgttggaa tggatatagc atgtgagtca tggcgccatg
    25441 gcgcaaaaag cgtgacggca gttgatattc aaaaaccact tgcctttggc aaagaaaaag
    25501 aaatggctga acgtttggga acaaaaattt tgtggccacg caatattgat taccttgatg
    25561 gttcccatgt gtactttaca aatggtgaag cactcccggc agatgttgtg tttattagta
    25621 ttggggaaaa gcctgatctg tcgtttttag gtgataccgt catggtaaat gagcgggggg
    25681 cgatagttac tgctgaaaaa tcatttaaaa gttcagaccc caaaatattt gccactggtg
    25741 atatcataaa acaagggctt attaccgatg caattgggat gggaagactt gctgccatgg
    25801 agatacacgc aacgctttca gatgaggatt ttatctatcc tgagaaaacc ctggtgccaa
    25861 aacggcgtat taatatagca tattttggtg gagagacacg tgaagttgac agatgtataa
    25921 gctgtggaac ctgtatttac tgcgataagt gtgtggaggc ctgtccacag ggagctatta
    25981 caagaaatgg tgaaatcttt acaatagatg cagagctgtg tacagcctgc tatacctgtg
    26041 tcaatgtttg tccccgtggt gcactgcagc caggtgattt tgatgctgtt gttcaggatg
    26101 tacttttcaa gaagtaatat ttttttaaat atatttgttt tatgattgat tgtttcatag
    26161 atgttaaata ttgaatgatt tattactatt tctaactaca ctattgactt tttatgagca
    26221 attga
//
LOCUS       DAORYL010000038        25812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1604_length_25812_cov_14.276507, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000038 DAORYL010000000
VERSION     DAORYL010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 25812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25812
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1604_length_25812_cov_14.276507"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..1568
                     /gene="lysS"
                     /locus_tag="PLV81_08360"
     CDS             84..1568
                     /gene="lysS"
                     /locus_tag="PLV81_08360"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOM10152.1"
                     /translation="MSEDINDLIVQRIEKVKALRAKGINPYPIRFKRTHTAVQIKELF
                     TEGQELQVKAAGRIKSKRVMGKASFAHIEDLSGLIQVYARLDRMGDEQYELFKTLDLG
                     DIIGLSGHVFKTKQGEITIEISDVVLLAKCIRPLPVVKEKDGRVFDEFADKEQRYRQR
                     YVDLIVNTQVRQQFILRSRLITGIRTFLNQKDFIEVETPMMQAIPGGAAARPFITHHN
                     ALDIDLYLRIAPELYLKRLLVGGFEKVYELNRNFRNEGISTRHNPEFTMLELYQAYAD
                     YNDMMDITQEMISTLAQQLLGSMVIQYQGNTIDLTPPWQKISYVDALKKYTGINFQEL
                     SHSEALRKAKEMGLDVDESMSKWKIAEEMFEEKVEANIIQPTFIIDYPKELSPLAKSR
                     DDNPEFVERFEVYIAGREMANAFSELNDPFDQRERFEQQVKMREAGDDEAQMMDIDYV
                     TALEYGMPPAGGLGIGIDRLAMLFIDTASIKDTILFPLLKPENR"
     gene            1574..2218
                     /locus_tag="PLV81_08365"
     CDS             1574..2218
                     /locus_tag="PLV81_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HOM10153.1"
                     /translation="MVAHYSYKQYGLHIHRFLYDWVLSWAHTPFGERALFVLALAESS
                     FFPVPPDVLLIALVLGKREAFVRLATLCSIASVIGGLVGYGIGYFLWYDGDSYSKIAL
                     FFFNNIPGFTVETFTAARQLYDAYSFWIVFTAGFTPIPYKVITITAGVANINIFIFVI
                     ASMISRTLRFFLVAGLIWKFGEPINAFINKWFNVLSIVFVILLFAGFYVLKYMV"
     gene            2246..3067
                     /locus_tag="PLV81_08370"
     CDS             2246..3067
                     /locus_tag="PLV81_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HOM10154.1"
                     /translation="MKIFIDSADIDEIAKAYEWGIVDGVTTNPSLLKTAVDRRKAKGE
                     KLDLEAYIVKLLEISKPNPVSLEVTKRTAQEMIEQGRNLYKRFNSIANNVNIKIPVNP
                     AMKSDEQCHFDGINAVKVLQSEGIPVNCTLVFTPEQALLAAKAGAAYISPFAGRIDDL
                     IRSRANIKFGKSDYFPEEGMEQNGVLLNDNGIMSGIDLVSQCVEILGYYDYQSQIIAA
                     SLRNPRQVREAALAGAHIATVPFGVIADMLKHEKTFEGMQKFTEDIVPEYEKLIG"
     gene            3139..3450
                     /gene="clpS"
                     /locus_tag="PLV81_08375"
     CDS             3139..3450
                     /gene="clpS"
                     /locus_tag="PLV81_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HOM10155.1"
                     /translation="MAHIFDNEGDLQVKKDIQVKKPRMYKVILHNDHYTTMDFVVEVL
                     VKIFHKNIMEATELMLQVHRNGFGICGVYTYDIAQTKANQVHSLARQREFPLKCTVEE
                     A"
     gene            3475..5703
                     /gene="clpA"
                     /locus_tag="PLV81_08380"
     CDS             3475..5703
                     /gene="clpA"
                     /locus_tag="PLV81_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HOM10156.1"
                     /translation="MEISTVLNQILMAAYNEAKNRKHEYLTPEHVLFASLYFPEGREV
                     IEGCGGNTEQLKRNLDKHLSTRIPVVEEGEPIQSVGLQHVIERAIMHINSAQKEILDF
                     GDIIIAIYEEEESFASYFLAREGITRYDLLNYISHGAQFDEDELEDFGDEIEEDTGKK
                     SKILEQFTVELTGKARRGELDPLIGREDILERTMQVLSRRLKNNPVHVGEPGVGKTAI
                     TEGLAQRIVEGTVPPVLKDAKIYRLDMGALIAGTRFRGDFEERLKRVIAELQKKEKVI
                     LFIDEIHTIVGAGAVSGGSMDASNILKPVLADGKIRCIGTTTYDEYRKYFEKDGALSR
                     RFQKIDIHEPSHEDTIKILQGIKSRYEEHHRVTYTDEALQQAVELSARYITERKLPDK
                     AIDVIDEAGAFARMYAHDDEEPIITAHDIERVIAKIARIPERTVSSSEIQKLQYLEDE
                     LKSQIFGQDGAVQKVVEAIKRSRAGFREAEKPIASFLFVGPTGVGKTELARQLSLIMG
                     IELIRFDMSEYQEKHTVARLIGAPPGYVGYEEGGQLTEAIRKHPYCVLLLDEIEKAHP
                     DVFNALLQIMDYATLTDNTGRKTDFRNTIIIMTSNAGVREMNKRVLGFEMATYNMDAV
                     NKELERLFAPEFRNRLDAIVYFNGINKEMMRKIVIKKVNEFAKQLEEKNVQLVCTDAC
                     IDWISEKAYSPVFGAREVARIIQEHIKSYFVDAVLFGSLKNGGRAIADVVNDLVSITI
                     EQ"
     gene            5705..6436
                     /gene="aat"
                     /locus_tag="PLV81_08385"
     CDS             5705..6436
                     /gene="aat"
                     /locus_tag="PLV81_08385"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOM10157.1"
                     /translation="MLYYLDKDVIWFPPVEEAIEDGLLAFGGDLTPQWLLEAYSRGIF
                     PWFNSDNSEILWWSPDPRFILFPQELRLHRSLKQVMKKKIFTVSMDTAFQKVIAACAM
                     PRKKESGTWITARMQKAYIKLHAMGFAHSVEVWHDGMLAGGLYGVSLGNAFFGESMFT
                     SVDNASKVALVTLNAVARRLGFAFIDCQVYTDNLKRFGARFIERQEFMTLLNASLKHS
                     THQGSWSSLIHDGYDYLECLTGAGA"
     gene            complement(6417..7319)
                     /locus_tag="PLV81_08390"
     CDS             complement(6417..7319)
                     /locus_tag="PLV81_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOM10158.1"
                     /translation="MDIAFKDYYKILDIPPNSDINTIKSAFRKKAKETHPDSTKDNNY
                     AQFVLIREAYDILTNTSQRASYDSAWKKYHAQTPKVETFFEDFGPPDDRYAQEWEYFV
                     LHPDDYLSRFESTIALIKASLKSIVISICIPLLVMAGILITVTIALLVLFVFAIAIAS
                     YSSIIAGIIITALMIKRLAEIIAELFTQYVKKGAAWMNEQLKGIPMKVGKMVFYGTFT
                     SAFLLLLGYAGAVIASAYRIVAAPYARIFILIIGVIAVAVVSLSLYFMFSVFVTALME
                     YPKIRYTKIKAKKENLLESKLLLQ"
     gene            complement(7335..7721)
                     /locus_tag="PLV81_08395"
     CDS             complement(7335..7721)
                     /locus_tag="PLV81_08395"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOM10159.1"
                     /translation="MSFEVEILNLSFINKDKIDYETIVFYVTYPEKIDIETSRQLWVF
                     LNTLINGGAKKIIIDLKKVSQIDSGGISTLISATKQIRKNNGDLVLARVNESLLNILD
                     VVRVGSFIKIFNTDVDAVQYFRYITI"
     gene            complement(7747..8124)
                     /locus_tag="PLV81_08400"
     CDS             complement(7747..8124)
                     /locus_tag="PLV81_08400"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOM10160.1"
                     /translation="MKCTICELPGIKSEDINFNRIVLIINLADVADMDKNDDLFMLLS
                     CLINGGLKKVVFDMTAVEFIDSYGMGTLIEITKLLRKQKDGDAVLINVAERIHLVFKP
                     IQLQKFMKIFNTIDEALHYFRYI"
     gene            complement(8188..10419)
                     /locus_tag="PLV81_08405"
     CDS             complement(8188..10419)
                     /locus_tag="PLV81_08405"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOM10161.1"
                     /translation="MKYNESVMLKPVYHNYTVFIISIASIIIIAYITALGFSYKFSRP
                     VEAIINALKKFDEGTTSIQIPVINQKDEIGKLAITTQHLLDMVNLEIKALTSTNMELL
                     RLHKLKDDFLANISHEIKIPLESITHLTNSIIHSYSTDETAAVTSSLEMISNSALRLS
                     YMIDDILDFTRLKNNDIILNKKVVDIAGVVNFVLSILQPVISSKSLSITIDINKKATH
                     VIADEQRLQQILLNIIGNAVKYSQKGTISIVTEKHNDTTLSIVVQDSAGGFPEQLIHH
                     IDDFGYDSKLQETPYGGLGLGLVITKKLMELHNGTIAIESIPDKGSTVTLYFPYDEAS
                     IDKELAATKSYIEYTIHPSGKDEHYLQSQFEKSQGFIYIVDDDPVNVKILYDMLTDAG
                     YYVEFSHEDSGLFSMLQQEKLPDIVLLDTVLPGTSAFQVCEKIRKRYSLYELPIIIIT
                     SKHRTQEILTAFRIGANDYLSKPFNKDELIARISNLIALKKSVEEHNEFVMIKHEIRL
                     AHEIHSSVVVQDIPRIKNMDIAYAYIPTREMGGDFYDVIKIDEEKTGIMIADVTGHGI
                     PAALVCAMLKMAFANNKEYAQHPAVLLTKLNKDLSNNIKENYITAAYALIDTKNKTVT
                     VSSAGHWSPLLIKINGSIFNNWPKGFPIGWVEDGTYEETTTTYSTGDKFLLYTDGIIE
                     ARNPDNKIFGFERLTDYINTNYLTNPQAFINGLINTLYTWGEMNQSDSFNDDVTIILA
                     ELS"
     gene            complement(10476..10895)
                     /locus_tag="PLV81_08410"
     CDS             complement(10476..10895)
                     /locus_tag="PLV81_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10162.1"
                     /translation="MKIRYKFFIAFIIIIAITTPVPIYILNRQIEQQHASIITEGTIR
                     AKQISHAILNMLLLNSGNIPQTSIDANEYISSFNPMFASGLICLQVILITPNPAYNGV
                     LLVDIPQGKTLSHIPPEHLQAIQKQPAVFFQLFREQN"
     gene            complement(10897..11973)
                     /locus_tag="PLV81_08415"
     CDS             complement(10897..11973)
                     /locus_tag="PLV81_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10163.1"
                     /translation="MKLKLINFLYVFCVITVVLLNCNIFAQTYFKDNVTVGTQAGYAV
                     ITGYYSDSLNNGLGAGFFSFVPLTDLLMLNGVLVYNRFSLANSSNSNMQSFGITITPS
                     LYYALPFNITMYAGAGLSLQYYTLSAIKTDAYDSTTKTGFLMNAGIAKTLFERTLLIA
                     ECMYHITELSHKYFQTTVFSIKAGYQFALHKPEYYISQEAEKNAVREQTVKKLYTAAM
                     DYVQKKDHVHALETFKQVLSLKPDHKESKQYVAAISQAKEQYDTAGILIKQDKYFDAL
                     PLLAASSQYIAEASIDYKNVQDKLKPQIPELQQKAVEAFNNKDYDACITIMNSVLLID
                     PDNTVAKGYIVRSQRIKETIQKLQ"
     gene            complement(12017..13027)
                     /locus_tag="PLV81_08420"
     CDS             complement(12017..13027)
                     /locus_tag="PLV81_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10164.1"
                     /translation="MKYTFPHFTRSITILIFMVIAVIYCISCTDDNFNTIIDQENAYT
                     NTFLDSTAPIPGNNGLLIVTNISENSAKVQWTLATDNSPPESLQYQVIVSSTLTLFSL
                     EEILQSGLLDEDKWSSEPVTEQIITNLLFGKQYFVNVCVCDPDNNISIYYPATFTTIG
                     RIYLFSAGTYTGAMASSSSTARQEINERIHIALTNTYSTLPQDNYVAFISIDSVDAII
                     NFPEHYGVPANWPIYSATGLLIAYNWNDLLDGTIIGELQNLGVCDSFWWSGSLQDGSC
                     DTDYTCNTWTSPTTLSSSSTLAVIPVLTGRSGAHNRTNYEWMYSNDRNCNDKLHLLGL
                     CW"
     gene            13640..13712
                     /locus_tag="PLV81_08425"
     tRNA            13640..13712
                     /locus_tag="PLV81_08425"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13673..13675,aa:Lys,seq:ttt)
     gene            13769..13841
                     /locus_tag="PLV81_08430"
     tRNA            13769..13841
                     /locus_tag="PLV81_08430"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13803..13805,aa:Glu,seq:ttc)
     gene            complement(13905..14369)
                     /locus_tag="PLV81_08435"
     CDS             complement(13905..14369)
                     /locus_tag="PLV81_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOM10165.1"
                     /translation="MPRIQLNALQAYNFSYYYKINVNDINLWGHVGIPEIIAIIHIVR
                     RDVLRSLGFDEHDLGDNSALIIADVAINLIKELHLDDEIIIETAIGEIGNKSFRFFHK
                     VCHDNSIAAIIEMGAVVIDRVNRKSTFISEEFLNKIVSFSDTILQEPPHCKK"
     gene            complement(14509..15384)
                     /locus_tag="PLV81_08440"
     CDS             complement(14509..15384)
                     /locus_tag="PLV81_08440"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOM10166.1"
                     /translation="MNKEYLLNHLESMWNRGLIECHATGNTRTYNAVPWIPGMYEVQL
                     KFIDKEFAHLHTKYIKTVGHYFLTPKPQIMQVIPIEQHVQSHSSPMPYEQLSAIINNS
                     RSFAVNECICKKQTALLNRGCNKPREVCLSISESPNYFDNHPHAGRIITKDEALSILK
                     MAEDAALVHMTANIQEGHYFICNCCGCCCVQLIAARFGMPETVNAHFYALINPELCTQ
                     CGTCETRCQIQAIQNTDGLRSIIQQKCIGCGLCVSTCPNNAITLIHKDKSQDTVPPKD
                     QDDWYREKSKSRYAI"
     gene            15897..16484
                     /locus_tag="PLV81_08445"
     CDS             15897..16484
                     /locus_tag="PLV81_08445"
                     /inference="COORDINATES: protein motif:HMM:NF028235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HOM10167.1"
                     /translation="MLPKHNAKHDIVAISASLMHLKGFNNTGIQEILQRANIPKGSFY
                     HYFKNKEDLGLQVIDYYTEMITSLFKQYLQDTTVLPLERLDNLMTFYAQHFKKMKYKL
                     GCPLGNFALEMGDLSEEFREKLYGSIDTLISIIESCLQEAKTEGQIADTIDTRKAAEF
                     IFQSFEGALLYMKVSKSDRPLLVCKDAVKRYVQGK"
     gene            16563..18803
                     /locus_tag="PLV81_08450"
     CDS             16563..18803
                     /locus_tag="PLV81_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOM10168.1"
                     /translation="MGQWKKTGCVLCAQNCGLEVEIENNTIVKVRGDKDNPRSKGYVC
                     RKGTNIMHHQHHDERLQYPLKRTDKGFVRISWDQAIEEIAAKLKEIIATYGPTSYAYV
                     GGGGQGCHFEAAFGVTLMRAIGSQYYYNALGQELTGLFWAYGRVAGKQYKFMIPDEHN
                     TDVLVAWGWNGMVSHQMPRAPLFLREFAKNPEKLLVVIDPRKSETAEIADIHLPIRPG
                     TDALLTKAMIGLILQEDWIDHAYIDKHVNGFEQITNWFADFDVKRAIEACGLTYEDVK
                     EVCRHIRFKKACVHSDLGVYMNRHSTLTTYLQIVLFAITGNLCVPGGNVIPGTLMPIV
                     SHSDERDEKTWRTVATGYPAIAGVFPPNVLPEEILNDNPERIRAVLCTQCNPLRSFAD
                     TAMYEKAFKTLDLLVVAEIAMTETAKLAHYVLPARTGYESWDGTFFTWTWPEIYFQMR
                     RPVVEPGGEQIELGEIHLRIADKMGLIPEIPQSLQEAADKDRLTFGMALMEYINTNPQ
                     ALKVIPFIIGKTLGRAMGSVHLASLWALLMNAPKSFKQCAARAGFSKGPLMGEDIFKA
                     IIENPQGLWIGKSDPLDNFSDLSTDDKKLHIHIPEMDDWCNEVTVENEIREIESRKEY
                     PFILSAGRHYPYNANTLMRNPAWNKGKRVCTCIMHPDDANELNMKDGDMVRVITEAGF
                     VELELEVTDQTARKYLMIPHGFGLEYGGKITGVNVNSLARNTHRDRIAGTPFHRYIPC
                     RVEKVS"
     gene            18847..19416
                     /locus_tag="PLV81_08455"
     CDS             18847..19416
                     /locus_tag="PLV81_08455"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit E
                     family protein"
                     /protein_id="HOM10169.1"
                     /translation="MKTICGYSIETYMSMVEKFHGSMAPGIIIGGFMVHTLWQHRNPE
                     KLYDFLCETSVCLPDAIQLLTPCTYGNGWLKVVHTGRFAMAMFDKLTGAGRRACLDAQ
                     KLEPYQEIKNWFFKLKPKKMQDKDLLINQIIAAGETILSVQDVQIGHTYLGKERLGPV
                     GICPDCGEAYPQKDGKRCIVCQGLTLYEK"
     gene            19406..20602
                     /locus_tag="PLV81_08460"
     CDS             19406..20602
                     /locus_tag="PLV81_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HOM10170.1"
                     /translation="MKSNRIQWLATKLQSIFKDITFDRNELSGAFGDIGTVIPLVVGV
                     LLATGMDAGKVFMAYGLMQIITGLVYKIPMPVQPLKVVALIVITQKLSADILLCGGLA
                     VGILMLVFTVSGLIDSFTKIIPKAVIRGIQFGLGLQLVMLSLKEYIPSERFSDYILVV
                     VAFLIAVAFMGNRRIPPALVLIPAGIFYGMIFNTGVHYQKLSHTITVVDALTFENIVN
                     GIVILALPQIPLSLGNSIFATSQLSHDLFPEKSVQPRKIAFTYSLMNIINPFIGGIAV
                     CHGSGGMAGHYAFGGRTGGSVIIFGALMITTGFIFGSDGIYIARLFPLPLLGVILFFE
                     ALTLLVLIGDVMHNSFDLKIALAVAALIVAIPYGYVVGMGIGICAYYLAKWIKAEKLA
                     SVNGKA"
     gene            20756..21937
                     /locus_tag="PLV81_08465"
     CDS             20756..21937
                     /locus_tag="PLV81_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOM10171.1"
                     /translation="MRLHYIIVVVAFILYKLSVVYAVTLSWDIPQNEKLEFVYDANIE
                     YYENGILRKIYGERNIIDLLCYEKDTKGQAVRGSFSVYRKNINDYVYRLEEQYFSDFY
                     INSNGIYVVPEGIFMPNLRHLPTFPETDVHVGEVWKAPVNIVFNNFSVPLILLLEAHY
                     TLLFINDQTKEAFIQYSILIDKTLKDKPYPEDLPIKIYGQYAGQVTWDLQKNQPKKSI
                     NAYNLLLIFGRTGELSTIEFKMSIDQTNKAYLHVPEEEKKKQQKEIEKALKQDGVSVD
                     SDARGMIIRFSDILFDFDKYNLNADARKVLDKLVEIIKAKYPNNEIIVEGHTDNIGTD
                     EYNQNLSEKRAQTVATILKNKLGHDKVSYRGMGKSRPIDDNSTAAGRQRNRRVEIIIK
                     M"
     gene            complement(21939..22682)
                     /locus_tag="PLV81_08470"
     CDS             complement(21939..22682)
                     /locus_tag="PLV81_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10172.1"
                     /translation="METHNTYHNLIDAIIDIEEDPDSREPAKGFPRLLCEYFFAEETS
                     ENKAIAGYIYQLPIPDVIKQKGLLKLTPEDIAQMVEGENLNDTLCARIMLQPAYLKYA
                     FPHHSPSFSKMPPDIKGEIIRLIKERNQMIVKAFEKMQQDIQATKERTMKTLIALILK
                     NVHLKTGMPFAKISEPVGQLIEKNFNFCNETFIASNKQIHEINDDAKIKNLLKSLFVV
                     KRFDELAELAQAFKTEAKRFTRRTQRILQ"
     gene            complement(22830..23825)
                     /gene="tsaD"
                     /locus_tag="PLV81_08475"
     CDS             complement(22830..23825)
                     /gene="tsaD"
                     /locus_tag="PLV81_08475"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOM10173.1"
                     /translation="MSNKILGIGLESSCDETAVAIVADGKTILSHAIFSQIHLHKEYY
                     GVVPEIASRAHLEVINDLIDHCLKEAKVTFKELSYVAATYRPGLVGSLLVALQSAKAI
                     SYTLSIPLIAVHHLEAHLYAPFLEGHGIEFPFIGLLVSGGNTALYCVHDYGNFELLGN
                     TTDDAVGEAFDKVAKFLSLGYPGGPIIEKLASQATLKKTIFPKILADSNDTYSFSYSG
                     LKTAVINYIHDNPDADISEIAYSFQERALEILVRRLFDASKRLKIHTLVIAGGVASNK
                     RLRELIFEKKKEYHTIFFPSPILCTDNGAMVAGIGYQYYKKGIYSPLSTDVKARP"
     gene            complement(23815..25182)
                     /locus_tag="PLV81_08480"
     CDS             complement(23815..25182)
                     /locus_tag="PLV81_08480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOM10174.1"
                     /translation="MIQWLRKRSFHIAFIAFLAGIFIGVNIAFTVSAQEPSFKYLDFF
                     HQVYQLIRNEYVEQPDSKNLFYGAIRGMIASLNDPFSRFLDEQEYSQLKEETSGEFIG
                     VGIEISAKNGDIIVVSPIEGTPAMKAGIQPGDIIYKIDSKPIKNKNINDIVSMIRGKP
                     HTKVILSIIREGIDEPIDVELERLPIKLQTVNYAVIDNTGVGYLRVKVFSEDTSTEVK
                     KALQYFQNNNIKKCIIDLRWNPGGLLDQAITISDMFLPKGTMIVSTKGRGGKETLQEY
                     RASSNPLFTGKCIILVNKGSASASEIVSGALKDNNRAKLLGEKTFGKGSVQKFFNLDE
                     NTGVTLTIAKYYTPSGISIHGIGIQPDYVVPMATISKDEQKQINMLIKEKILEKFIST
                     HTKYDEPTKQEFKKLLEKNSIKLSDSTAYFILKSEIYRYTKRPLYDLEFDNQLTEAIK
                     IINEQ"
     gene            complement(25199..25474)
                     /locus_tag="PLV81_08485"
     CDS             complement(25199..25474)
                     /locus_tag="PLV81_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10175.1"
                     /translation="MKKIFALILCIMLGYFTACIGEKLSEEEFSILWQEYLSREFIES
                     FDEQQSSKQRREIMDTVLHDFKVSQQAFYSYCKTKHPDKYKLFDVNP"
BASE COUNT         7945 a         4533 c         5275 g         8059 t
ORIGIN      
        1 cagtaactat cgcttgacat aaaaagatga aaatatatgg ttttatagat ataatactat
       61 ttatttccag gggataacag gtaatgagtg aagacattaa tgatttgatt gtacagcgca
      121 ttgaaaaggt aaaagctctt cgtgcaaaag gtataaatcc gtatccaata cgatttaaac
      181 gaactcatac agcagtgcag attaaagagt tatttactga agggcaggaa ttgcaggtaa
      241 aggctgctgg caggataaag tccaaacgcg ttatgggcaa ggcaagcttt gcgcatattg
      301 aagatttaag cgggcttatc caggtatatg cgcgccttga caggatgggt gatgaacaat
      361 atgaattgtt taagacgttg gaccttggcg atattattgg cctatcgggt catgtgttta
      421 aaaccaaaca gggtgaaata accattgaaa ttagcgatgt agtgttgctt gctaaatgta
      481 tacggccgct tcctgttgtt aaggaaaagg atggcagggt ttttgacgaa tttgcggata
      541 aagagcagcg atacaggcag cgctacgtgg atttgattgt caatacacag gtgcgccagc
      601 agtttattct cagaagcagg ctcataactg gtatacggac gtttttaaat caaaaagatt
      661 ttattgaagt ggaaacacca atgatgcagg ccatacccgg tggtgcggcg gctcgtccgt
      721 ttattactca tcacaatgca ctggatattg acctgtactt acgcattgcg ccggagttat
      781 atttaaaaag gcttctggtt ggtggatttg agaaggtgta tgagttaaat cgcaatttcc
      841 gcaatgaagg aatatcaacc cgccataatc cagagttcac catgcttgaa ttgtaccagg
      901 cgtatgcgga ttacaacgat atgatggaca taacccagga gatgataagt acactggctc
      961 agcagttact gggtagtatg gtcatccagt atcagggtaa caccattgac ctcacgccgc
     1021 cgtggcaaaa gatttcatat gttgatgcgc tgaaaaaata cactggcatt aatttccagg
     1081 aactatcgca cagtgaagcg ttaagaaaag caaaggaaat gggcctggat gttgatgaaa
     1141 gcatgtctaa gtggaaaata gcagaagaga tgtttgaaga aaaggttgag gctaatatca
     1201 tacaacctac ttttatcata gactatccca aggaactatc gccccttgca aaaagcaggg
     1261 atgataatcc tgagtttgtt gagcgctttg aggtatatat tgcaggaagg gaaatggcta
     1321 atgcattcag tgaattaaat gacccatttg accagcgtga gcgctttgaa cagcaggtaa
     1381 agatgcgcga ggctggcgat gatgaggcac agatgatgga cattgattac gttacggcgc
     1441 ttgaatatgg tatgccaccg gcaggtggcc ttggcattgg catagacagg ctggccatgc
     1501 ttttcattga tactgcgtca attaaggata ccattttgtt cccgctgtta aaaccagaaa
     1561 accggtagtg tatatggttg cccattattc ttataaacag tatggattgc atattcacag
     1621 gtttttatat gactgggtcc tttcgtgggc gcatacacca tttggtgagc gtgcattatt
     1681 tgtacttgca ctggccgagt cgtctttttt cccggttccg cctgatgtgc tgctcattgc
     1741 gctggtactt ggcaaacgag aggcatttgt gcggcttgca acactgtgct cgattgcttc
     1801 agtaattggg gggcttgtcg ggtatggtat tggatatttt ttatggtatg atggcgatag
     1861 ttattcaaaa atagccctat tcttttttaa taatatcccg gggtttacgg ttgaaacatt
     1921 tactgctgcc cgtcagctat atgatgccta ttcattctgg atagtgttta ctgccgggtt
     1981 tacgcctatt ccgtataaag tcataaccat caccgcaggt gttgccaata tcaatatatt
     2041 tatatttgta atagcttcga tgatcagccg tacactgagg tttttccttg ttgccgggct
     2101 tatatggaaa tttggcgaac caattaatgc gtttattaat aaatggttta atgtattatc
     2161 tatagtgttt gttatcctgc tatttgcagg tttttatgtg ttaaagtata tggtttagac
     2221 attttagatt taatggagga ttggtatgaa gatttttatt gatagtgcgg atattgatga
     2281 gatagcaaag gcgtatgaat ggggtattgt tgatggcgta acaactaacc cttcgttgct
     2341 taagactgct gttgatagaa gaaaagctaa aggcgaaaag cttgacctgg aagcatatat
     2401 tgtaaagctt ttagaaatat caaaacccaa cccggtgagt ttagaagtaa caaagcgcac
     2461 tgcccaggaa atgatagagc agggacgcaa cctgtataag cgcttcaaca gtattgcaaa
     2521 caatgttaat ataaaaattc cggttaatcc tgccatgaaa tctgatgagc agtgtcactt
     2581 tgatggaatt aatgcagtaa aagtgttaca gtcggagggc attccggtta actgtactct
     2641 tgtctttact cctgaacagg cattactggc agcaaaggct ggggcggcat atatcagtcc
     2701 atttgctggc cgtattgatg accttatccg cagcagggca aatatcaaat ttggtaaaag
     2761 tgattatttc cctgaggaag gcatggaaca gaatggtgta ttgctcaatg ataatggtat
     2821 tatgtcagga attgacctgg tttcacagtg tgtggaaata ctgggatact atgactatca
     2881 atcacagatc attgctgcat cattgcgcaa tccacggcag gtaagagaag ctgcacttgc
     2941 aggtgcacat atagctacag taccatttgg ggtcatagct gacatgctga aacatgaaaa
     3001 aacatttgaa ggtatgcaga aatttactga agatattgtt ccagaatatg aaaaactgat
     3061 tggataagat acttgccaaa ttctaaatat ttttttatat tttgtatgtt agctctatca
     3121 taaaccacgg ggataaccat ggcacatata tttgataatg aaggtgatct ccaggtaaag
     3181 aaagacatac aggttaaaaa gcccaggatg tataaggtca tacttcacaa tgaccattat
     3241 acaaccatgg attttgttgt tgaggttctg gttaagatat tccataaaaa cattatggaa
     3301 gctacagaac tgatgttaca ggttcacagg aatggttttg gtatatgcgg agtatataca
     3361 tatgacattg cgcagaccaa agcaaatcag gtgcattcat tagcacggca gcgggagttt
     3421 ccgctcaagt gcactgttga agaagcataa ttaaataatt cagggcattt tccaatggaa
     3481 ataagtacag tacttaatca gatattaatg gctgcctaca atgaggcaaa aaaccgtaaa
     3541 catgaatatc ttactcctga gcatgtatta tttgcatctc tgtactttcc tgaaggcagg
     3601 gaagtaatag aaggatgtgg cggtaatact gagcaactca agcgaaattt agataaacat
     3661 ctttcaaccc gcataccggt ggttgaagaa ggtgagccaa ttcaatctgt tgggctgcag
     3721 catgtcattg aacgtgcaat tatgcatatt aattctgctc aaaaagagat acttgatttt
     3781 ggagatatca tcattgccat ttacgaagaa gaagagtcat ttgcatcata ttttctggca
     3841 cgagagggaa taacgcggta tgacctttta aattatattt ctcacggagc acagtttgat
     3901 gaagatgaac tggaagactt tggcgatgaa attgaagagg atacaggaaa gaaaagcaaa
     3961 atacttgagc agtttactgt tgagttgact ggaaaagctc gcaggggtga gcttgatccg
     4021 ctgattggta gagaggatat cctggagcgg acaatgcagg tgctatcgcg caggcttaaa
     4081 aacaaccctg tccatgttgg agagcctggt gtgggcaaaa ccgcaattac cgaagggctt
     4141 gcacagagga tagtggaagg caccgtacca cctgttctta aagatgcaaa aatttatcgg
     4201 cttgatatgg gtgctcttat agccggcaca cgattccggg gcgattttga agaacgtctt
     4261 aaacgggtta ttgcagaatt acaaaaaaaa gaaaaggtta tcctttttat tgatgagata
     4321 cataccatag tgggtgctgg agctgtcagt ggtggatcaa tggatgcttc caatatatta
     4381 aagccggtgc ttgctgatgg caaaatacga tgtattggca caaccaccta tgatgaatac
     4441 agaaagtact ttgaaaaaga cggtgcatta tcgcgacgtt tccagaagat tgatattcat
     4501 gagccatcgc atgaagatac aataaaaata ttgcagggga taaaatcgcg gtacgaagag
     4561 catcacaggg ttacctatac cgatgaagca ttacagcagg cggtggaact ttcagcacgc
     4621 tacatcactg aacgaaagct tcctgataaa gccatagatg ttatagacga ggcgggtgca
     4681 tttgcccgca tgtatgcaca tgatgatgaa gagccaatta tcactgccca tgatattgaa
     4741 cgggtaattg caaagatagc acgcatacct gaaagaacag tttcatccag tgaaattcaa
     4801 aaattgcagt atttagaaga cgagttgaaa tcacagattt ttgggcagga tggtgcagtg
     4861 cagaaggttg tagaggccat aaagcgctca cgcgctggat tcagggaagc tgaaaaaccc
     4921 attgcttcat ttttatttgt tggaccaaca ggagttggca aaactgagct tgcacggcag
     4981 ctatcgttga ttatgggtat tgaacttata cgttttgata tgagcgagta tcaggaaaaa
     5041 catactgtgg cacggcttat aggagcacca ccggggtatg ttggatatga agaaggcggt
     5101 cagctgactg aagccattcg taagcaccct tactgtgtat tattacttga tgagattgaa
     5161 aaagcacacc ctgatgtatt caatgcactg ttacagataa tggattatgc aaccttgact
     5221 gataacacag ggcgaaagac tgatttccgc aataccatta ttatcatgac atctaatgca
     5281 ggcgtgcgcg aaatgaacaa gagggtcctt ggctttgaaa tggctaccta taatatggat
     5341 gcggtcaata aagaattaga gcggctgttt gcgcctgaat tcagaaaccg ccttgacgcc
     5401 atagtgtatt tcaatggcat caataaagaa atgatgcgca agatagttat aaagaaggta
     5461 aacgaatttg caaaacagct tgaagaaaaa aatgtgcagc ttgtctgtac ggatgcatgt
     5521 atcgactgga tttcagaaaa agcatattct cccgtatttg gcgcacgtga ggttgcccgc
     5581 atcatacagg aacatatcaa gtcatacttt gtggatgctg tgttatttgg gtcacttaaa
     5641 aatggtggca gggcaatagc tgatgtagta aatgatctgg tatccataac tatcgaacaa
     5701 taatatgttg tattacctgg ataaggatgt catctggttc ccgccggtgg aggaagccat
     5761 agaagatggg ttacttgcat ttggtggaga tttaacacca cagtggttgc ttgaggccta
     5821 cagcagaggc atttttccat ggtttaatag tgataactct gagatattat ggtggagccc
     5881 cgatccgcgt tttattttgt tcccgcaaga attaaggcta catcgttcat taaagcaggt
     5941 aatgaaaaaa aagattttta ccgtaagtat ggacacagca tttcaaaagg ttatagctgc
     6001 atgtgccatg ccacggaaaa aagaatctgg tacctggata actgcacgaa tgcaaaaagc
     6061 atatataaag cttcatgcta tgggttttgc acattctgtt gaggtgtggc atgacggcat
     6121 gcttgcaggg ggcctgtatg gggtgtcttt agggaatgca ttctttggtg aatccatgtt
     6181 taccagtgtt gataatgctt caaaggttgc tctggttact ctgaatgcag ttgcacgaag
     6241 attgggtttt gcatttattg actgccaggt atatacagat aatcttaaac gctttggtgc
     6301 gcggtttatt gagcgacaag aatttatgac cctgctcaat gcttccctga aacattcaac
     6361 acatcagggc agctggagtt cactgataca cgatggctat gattatcttg aatgccttac
     6421 tggagcagga gcttagattc aagtaaattt tctttttttg cttttatctt tgtgtaacgg
     6481 atttttggat actccataag tgcggttaca aaaacagaaa acataaaata cagtgacagt
     6541 gaaacaacag caactgcaat caccccaatg ataagaataa atatcctggc atatggtgct
     6601 gctacaatac ggtatgctga agcaataacc gcacctgcat atcccagcaa aagcaggaat
     6661 gctgatgtaa atgtgccata gaataccatt ttgcccacct tcattggtat gcctttaagc
     6721 tgttcattca tccatgcagc accttttttt acatactgtg taaaaagctc agcaataatt
     6781 tcagccagcc gttttatcat caatgcagtt atgattattc cagcaatgat tgatgagtat
     6841 gatgctattg ctatggcaaa aacaaaaaga acaagcaggg cgatagttac tgtaataaga
     6901 atacctgcca tgacaagaag aggaatgcat atactgataa caattgactt cagcgaagct
     6961 ttaatcagtg cgatggtgct ttcaaaacga cttaagtaat cgtcagggtg aagtacaaag
     7021 tattcccatt cctgtgcgta ccgatcgtca ggtggaccaa aatcttcaaa aaatgtctcc
     7081 acttttggag tttgcgcatg atatttcttc catgctgagt cataacttgc acgctgagat
     7141 gtgtttgtta aaatgtcata tgcttcacgg ataaggacaa attgtgcata attattgtct
     7201 ttggtgctgt caggatgggt ttcttttgct tttttcctga acgccgattt gatggtatta
     7261 atatctgaat tggggggaat atcaagaatc ttatagtaat ctttaaatgc tatgtccata
     7321 agcatcagga gatattatat agttatatac ctgaaatact ggacagcgtc cacatcagta
     7381 ttgaatattt ttatgaaact tccaacccgt accacatcta aaatattcag taaagattca
     7441 ttaacacgag ctagcacaag atctccatta ttttttctga tttgttttgt agctgaaatc
     7501 agggtactta tgccaccgct atcaatctgg gatacctttt ttaaatcaat aattattttt
     7561 tttgccccac cattgatcag ggtgtttaag aatacccaca attgtcttga cgtttcaata
     7621 tcaatttttt cagggtacgt aacataaaag acaattgttt cataatcaat tttatctttg
     7681 ttgatgaacg atagatttaa tatttcaact tcaaaactca tactgtacca ccattaaaat
     7741 aatatattag atataacgga aataatgcaa tgcttcatct atagtgttaa atatcttcat
     7801 aaatttttga agctgtattg gtttaaatac aagatgaatt ctttcggcca catttatcag
     7861 tactgcatca ccatcttttt gtttacgtaa taatttggtt atttcaatta atgttcccat
     7921 accataacta tcaataaatt caactgctgt catatcaaat acaacttttt ttaagccacc
     7981 attaatcagg caggacaaaa gcataaacag gtcgtcattt ttgtccatat cagcgacgtc
     8041 agctaaattt ataatcagga caatccgatt aaagttgata tcctcgcttt ttatacctgg
     8101 caattcacat attgtgcatt tcatacatat agtatgttac attattatca taaaaaatca
     8161 acaataaaac aaaatcatct atcataatca ggataattct gctaaaatta tagtaacgtc
     8221 atcattaaat gagtctgatt gattcatttc cccccatgtg tataatgtat taatcaaacc
     8281 attaataaaa gcttgaggat tggtgagata gttagtgtta atataatctg taagcctctc
     8341 gaagccaaat attttgttat caggatttct ggcctctatt atcccatcag tgtaaagtaa
     8401 gaatttatct ccggttgaat aggtagttgt tgtttcttca tatgtaccat cttctaccca
     8461 tcctatgggg aaaccttttg gccagttgtt gaatattgag ccatttattt taataagcaa
     8521 tggggaccag tggcctgctg aagatactgt tacagttttg tttttggtat caattaaggc
     8581 atatgcagca gtgatgtaat tttcttttat gttattactc aaatctttat taagtttagt
     8641 aagcagaact gccggatgct gtgcatattc cttattgtta gcaaaagcca tttttaacat
     8701 ggcacaaaca agagcagcag gaatgccatg acctgttaca tcggctatca taataccggt
     8761 tttttcttca tcaattttaa taacatcata aaaatcaccg cccatttctc tggtagggat
     8821 gtaagcgtag gcaatatcca tgttttttat cctgggtata tcctgtacta ccactgaact
     8881 gtgtatctcg tgtgcaaggc gtatttcatg ctttatcatg acaaattcat tatgctcttc
     8941 tactgacttt ttcagggcaa taagatttga aatgcgtgca attaattcat ctttgttgaa
     9001 gggtttgctg agataatcat ttgcaccaat cctgaaagct gtcagtattt cctgagtgcg
     9061 gtgctttgat gtaattataa ttattggtag ctcatataac gaataacgtt tacgaatttt
     9121 ttcacatacc tggaatgcag aagttcctgg caaaactgta tccagcaata cgatatcagg
     9181 caacttttcc tgctgtaaca tagaaaatag gccagaatct tcatgtgaaa attctacata
     9241 atagcctgca tctgtcagca tatcatacag tattttgaca ttaaccgggt catcatcaac
     9301 gatataaata aatccctgtg atttttcaaa ttggctttgc agataatgct catctttacc
     9361 tgaaggatgt atggtatatt caatataaga tttagttgct gctaattctt tatcaatact
     9421 tgcttcatca taaggaaaat acaaagttac tgttgaacct ttatcaggta ttgattctat
     9481 tgcaattgtg ccattgtgca attccatcag tttttttgta ataacaaggc caagccccaa
     9541 tcctccatat ggggtttctt gaagtttact atcgtatcca aaatcatcaa tatgatgaat
     9601 taattgctcc ggaaatcctc ctgcactatc ctgtactaca atagaaaggg tagtatcatt
     9661 gtgtttttct gtaacaatgg atatggtacc tttttgtgaa tatttaactg cattgcctat
     9721 aatattcaac agtatctgtt gcaatctttg ttcatcagca atgacgtggg ttgctttttt
     9781 attaatgtct attgtaattg acagggattt gctgctaata accggctgta atattgataa
     9841 tacaaaatta actacacctg ctatatcaac aactttctta ttcagaataa tatcattatt
     9901 tttcaaccgg gtaaaatcca gtatatcgtc aatcatatac gataatctta aggcactatt
     9961 agaaatcatt tccagagaac ttgtaacagc agcggtttca tcggtagagt agctatggat
    10021 aatagaattt gttaaatgtg ttatgctttc aagaggtatt ttaatctcat gcgatatatt
    10081 ggccaggaaa tcatctttga gtttatgaag ccgtaacaat tccatattgg ttgaagttaa
    10141 tgcctttatt tctaaattaa ccatatctaa caggtgctga gttgtaattg ccagttttcc
    10201 aatttcgtct ttctggttta ttacaggaat ctgaatggat gttgtacctt catcaaattt
    10261 ctttaaagca ttaataatag cttccacagg tctggaaaat ttatagctaa atccaagtgc
    10321 agtaatatat gcaataatga taatggaagc aatacttatg atgaatacgg tataattatg
    10381 gtatacgggt tttagcatca ctgattcatt gtatttcata tgcgttaaac atgctattgc
    10441 actgtttgcc agggatgttt gttcagtaaa ttcaatcagt tttgttctct gaatagttga
    10501 aaaaacacag ccggttgttt ttggatagct tgtaaatgtt ctggtggtat atgtgacagt
    10561 gttttacctt gaggaatatc aacaagcaat acaccattat atgccggatt tggtgtaatc
    10621 agaataacct gaaggcaaat cagccctgag gcaaacatgg ggttaaaact gcttatgtat
    10681 tcattggcat ctatactggt ctgaggaata ttgccactgt ttaataacag catgttaagt
    10741 attgcatgtg atatctgttt tgctcgtata gtcccttcag taataatgct ggcatgctgt
    10801 tgttctatct gtctgttgag tatatatata ggtacaggag tggtaatggc aataatgata
    10861 ataaatgcaa taaagaattt ataccgtatt ttcatgttat tgcagcttct gtatggtttc
    10921 ttttattcgt tgtgaccgta caatataacc tttagcaacc gtattatcag gatctatcaa
    10981 aagtaccgaa ttcattattg ttatacatgc gtcatagtct ttgttattga atgcttcaac
    11041 agctttttgc tgaagttcgg gaatctgagg ttttagttta tcctgtacat ttttataatc
    11101 aatggaagcc tctgcaatgt attgagatga agcagctaaa agcggaagtg catcaaaata
    11161 tttatcctgc tttatgagta tccctgcagt atcatattgt tctttggcct gagatattgc
    11221 agcaacatac tgcttggatt ctttgtggtc aggtttaagc gatagtacct gcttaaatgt
    11281 ttccagcgca tgtacatggt cttttttttg tacatagtcc attgcagcag tataaagctt
    11341 tttaacggtt tgttcccgta ctgcattctt ttcagcttct tgagaaatat aatactctgg
    11401 cttatgtagt gcaaactgat acccggcttt aattgaaaaa acggtagttt gaaaatattt
    11461 gtgcgatagc tccgttatat gatacataca ttcagcaatg agtagcgtcc gttcaaatag
    11521 agtcttggct attcctgcat tcatcaaaaa accagttttt gtggtggaat catatgcatc
    11581 agtttttata gcagaaagag tataatactg taaagacaac ccggcaccag catacatcgt
    11641 aatattaaag ggaagcgcat aatacagtga tggagtaata gttatgccaa acgactgcat
    11701 gttagaattg ctgctgttag caagtgaaaa tcgattgtac acaagcactc cgttaagcat
    11761 taataaatca gttaaaggta caaacgaaaa aaatccagca cccaaaccat tattgagact
    11821 atcactgtaa tagcctgtta ttacagcata ccctgcctgt gtgccaactg ttacattatc
    11881 tttaaaatat gtttgcgcaa atatattgca attaagtaag acgacagtaa tgacacaaaa
    11941 tacatacaaa aaatttataa gctttaattt cacctgtatt taccagcatg attgtaatat
    12001 atattgaata ctactattac cagcacaaac ctaaaagatg taatttgtca ttgcaatttc
    12061 ggtcattact atacatccat tcataattag ttctgttatg tgccccactt cgtcctgtga
    12121 gtacgggtat aacagctagt gttgaacttg acgataaagt tgttggagat gtccatgtat
    12181 tgcaggtata gtcagtatcg caggaaccat cctgcagact gcccgaccac caaaaactat
    12241 cgcatacgcc taaattttgt agttcaccaa taattgtacc atccagtaga tcattccagt
    12301 tatatgctat taataaaccg gtagctgaat aaataggcca gtttgcagga acgccataat
    12361 gttctggaaa attaattatt gcatcaacac tgtcaatact gatgaaagca acataattat
    12421 cctgaggcag tgtagaataa gtatttgtga gtgcaatatg aatacgttca ttaatttcct
    12481 ggcgtgctgt actgctggat gaagccatgg caccggtgta tgtgccagca ctgaaaagat
    12541 atattctgcc aatagtggta aatgttgcag gataatagat agatatattg ttatcaggat
    12601 cgcatacaca gacatttaca aaatattgct tcccaaataa taagtttgtg attatttgtt
    12661 cagttacagg ctcactggac catttatctt catcaagaag tccggactga agaatttctt
    12721 ccaatgaaaa aagagtcaat gtgctactaa caataacctg gtattgtaat gattcagggg
    12781 gagagttgtc agttgccaac gtccactgta cttttgctga attttcagaa atattagtta
    12841 ctattaagag cccattattg ccaggtatag gtgcagtact atccaaaaat gtattagtat
    12901 atgcattttc ctggtctatg atagtattaa agttgtcatc tgtacatgat atacaataaa
    12961 taacagcgat aaccataaat attaaaatgg ttatgcttct ggtaaaatgt ggaaatgtat
    13021 atttcataac tgtaacatgg taaaaatggc tataaaaaga aaattacccc aaaccatatt
    13081 aagttaataa aattgccaat attttacaac ttttttagca attattttta aaaatttttt
    13141 aaaatcgtta gttatacata tcgtaaatat ataaacaata tttatacata tgtacaataa
    13201 tacggattga gtcaaactgt tcttacatgt atgtattaaa aatatttata tagaaataaa
    13261 cccttcgtcc aacctttacg gggttgaata gtgatgtaaa agatgtgcta ttttatataa
    13321 aatttcggca gtaatttcat tcaattgtaa tttttatgaa tactcttcag agtatagcaa
    13381 tgatttatat cactttacct ttcacggcag agttaataga gcttttctat cctgggaaac
    13441 gtaataaagc aatataatga attataattg ctattattaa acttttttat gaaaaatttt
    13501 caaaatggtg taattttttg aaaagcaaga ataattgaag tattttttat ttgacactaa
    13561 aatactatta tttatataag gttcattcat ttttttattt gcaatgtaat ataaaatacg
    13621 caaaagacgt tttttgcagg agtcgctagc tcagtcggta gagcaactgc cttttaagca
    13681 gtgggtccgg agttcgattc tccggcgact caataaaata atattgacag tttcaatatt
    13741 attttttatc tggtcaacat atttaattgt ccccgtcgtc taggggtcta ggacaccggg
    13801 ttttcatccc ggcaacaggg gttcgaatcc cctcggggat gcatttacaa agggagtgtc
    13861 tcaaaagacg ctcccttttt taataattat tttacaaatg gttattattt cttacaatgt
    13921 ggaggttcct gcagtattgt atcagaaaaa ctaactattt tatttaaaaa ttcttctgaa
    13981 ataaatgtgg attttcgatt gactctgtct atgaccactg cacccatttc aataatagct
    14041 gcaatggaat tatcatgaca aactttatga aaaaagcgaa agcttttatt tccaatttcc
    14101 cctattgcag tttcaattat tatttcatca tcaaggtgta attctttaat cagatttata
    14161 gcaacatcag ctataataag agcagaattg tcccctaagt catgttcatc aaaacccagt
    14221 gaacgaagta catctctccg tactatatga atgattgcaa taatttctgg tatacctaca
    14281 tgtccccaca ggttaatgtc gttaacgtta atcttatagt aatatgaaaa attataagcc
    14341 tggagtgcat tgagctgtat tcgtggcata taacaacaac cttacgttag ttttttgaat
    14401 tattttgtaa taaacaaatt cttatatctc attgtcaata gtaatttatt cataatagcg
    14461 aaacattgtt acgttgtatg aataaatagt attgcttgat gaagtttatt atatagcgta
    14521 gcgtgattta cttttttccc tgtaccagtc atcctgatcc tttggtggca ctgtatcctg
    14581 agatttgtct ttatgaataa gggtaattgc attatttgga caagtagaaa cacaaagccc
    14641 gcatccaatg catttttgct gtataataga acgcagccca tcggtattct gtatagcctg
    14701 aatctggcat cgtgtttcac aggtgccgca ttgtgtacac aattcaggat taatgagtgc
    14761 ataaaagtga gcatttactg tttcaggcat cccaaatcga gctgcaatga gctgaacgca
    14821 gcaacagcca caacaattgc atataaaata atgcccttcc tgtatattgg ctgtcatatg
    14881 gacaagtgca gcatcttctg ccattttgag tattgatagc gcctcatctt tggtgataat
    14941 tcttcctgca tgaggatgat tatcaaaata attgggtgat tctgagatag ataagcatac
    15001 ttcgcgtggt ttattgcatc ctctattaag aagagccgtc tgctttttgc atatacattc
    15061 attaactgca aatgatctgg agttattaat tatggcagat aactgttcat acggcatggg
    15121 tgatgagtga gattgaacat gctgttcaat ggggattacc tgcataatct gtggtttggg
    15181 tgttaaaaag taatggccaa cagtttttat gtattttgta tgaagatgtg caaattcttt
    15241 gtctataaac tttagctgca cctcatacat gccaggtatc catggtactg cattataggt
    15301 tctggtgttg ccagtggcgt gacattcaat aagtcctcga ttccacatac tttctaaatg
    15361 gttcaataga tattctttat tcattccagt acgagcagct atctgatcta tcgtttcctt
    15421 ttccagagta agtttgcaaa aaagcttgcc atctacgggt gtagaaaatt ttttgcaagc
    15481 tcaagttcaa tgccggtatc agtacaaaga aaaccggtag acattgaatt gcaataaaga
    15541 gctaattaat ggtaactatc gcttataaaa acatctgaag cctggtattg aatttatacg
    15601 gtatatgcat gtaagtgttg agttatttat ataaataatt accgggataa ataaagaaca
    15661 gaaagaataa tttcaaaaat gtatgaaaca aaaaaataca gcaaacacca tgaccatgct
    15721 aaggtcatgc ataaattgta gatagtgttg ctttgggaat acattggtaa aaagatggga
    15781 aatgccataa cgtgaaaatt tttgtaaaaa ttttacaata ggggtggaaa aattcttgac
    15841 aaagagacga ccggtctact atatgaagaa ttggaaacca ttgaagggtt agggctatgc
    15901 ttccaaaaca caatgcaaaa catgatattg ttgccataag cgcaagtctt atgcatttga
    15961 agggttttaa taataccgga atacaggaaa tcctgcaaag ggctaatata cctaaaggct
    16021 cattttatca ttatttcaaa aataaagagg atttaggact tcaggttatt gattattata
    16081 ctgaaatgat tacttcacta tttaaacagt atttgcagga taccaccgtt ctgccgcttg
    16141 aaaggctgga taatcttatg acattttacg cgcagcattt taaaaagatg aaatataagt
    16201 tgggctgtcc cctgggtaat tttgcattgg agatgggcga tctgagcgaa gaattcagag
    16261 aaaaactgta tggctcaata gatacgctta tttcgatcat tgaatcatgt ctacaggagg
    16321 ctaaaacaga gggtcagatt gctgacacta ttgatacgcg taaggctgct gaatttattt
    16381 ttcagagttt tgaaggggcg ttgctttaca tgaaagtttc taagagtgac aggcccttac
    16441 tggtatgtaa agatgccgtt aagcgctatg tacagggaaa atgagcaatc gtcatccaga
    16501 gcggtaccag gtcattctga aggaagcgaa ggatctctaa cttttatagt atggagggta
    16561 gtatgggtca gtggaagaaa acaggttgtg tgttgtgcgc acaaaactgc gggcttgagg
    16621 ttgaaataga aaataacacc attgtgaaag tgcgcggcga taaagataat ccccgcagca
    16681 aaggatatgt gtgcaggaaa ggtaccaata taatgcatca tcaacaccat gatgaacggt
    16741 tacagtatcc attaaaacga actgataaag gttttgtgcg tatttcatgg gatcaggcaa
    16801 ttgaagaaat agctgcaaaa ctaaaagaaa ttatcgccac ctatggacct acgtcatacg
    16861 catacgttgg tggcggtggg cagggctgtc attttgaggc agcctttggt gttactttaa
    16921 tgagagctat cggctcccag tattactaca atgcgctggg tcaggaactc accggcttat
    16981 tctgggcata cggcagggtt gcagggaagc aatacaaatt catgattccc gacgaacata
    17041 atactgatgt gctggtagca tggggatgga atgggatggt gagccatcag atgccaaggg
    17101 cgccgctttt tttgcgtgaa tttgcaaaaa atcctgaaaa gcttcttgtg gtgattgatc
    17161 cacgaaaatc agaaaccgca gaaattgccg atattcacct gccaatacgg cccggtaccg
    17221 atgccctgct taccaaagcc atgattgggc tcatactgca ggaagactgg attgaccatg
    17281 cgtacattga caaacacgta aatggatttg aacagattac aaactggttt gcagattttg
    17341 atgtcaaaag agccattgaa gcgtgtggcc tcacgtacga agatgtaaag gaggtatgca
    17401 ggcacatacg gtttaaaaag gcatgcgttc actccgactt aggtgtgtat atgaatagac
    17461 atagcacgct gactacatat ttacagatag tgctatttgc tatcactgga aacctgtgcg
    17521 tccctggcgg taatgtaata ccgggaacgc tgatgccaat tgtatcgcac agtgatgagc
    17581 gcgatgaaaa aacatggcgc accgtggcaa ccggatatcc ggctattgca ggggtgttcc
    17641 cacccaatgt gctaccagaa gaaatattaa acgataatcc cgaacgaatc agagcggtac
    17701 tatgcaccca gtgcaatccg ctgcgctcgt ttgccgacac ggccatgtat gaaaaggctt
    17761 ttaaaaccct tgaccttctg gttgttgcag aaattgcgat gactgaaacg gcaaagctgg
    17821 ctcattatgt gctccctgcc cggacgggat atgaatcgtg ggatgggaca tttttcacat
    17881 ggacatggcc ggaaatttat ttccagatgc ggcgtccggt tgttgagccc ggcggtgagc
    17941 agattgaact gggcgagatt catttacgca ttgccgacaa aatgggcctt ataccggaaa
    18001 tacctcaatc gctccaggaa gcagcggata aagacagact aacatttggc atggcgctta
    18061 tggagtatat caatacaaat ccacaggcat tgaaggttat accctttata attgggaaaa
    18121 cgctgggcag ggcaatgggt tcggttcatc ttgccagttt gtgggcgctt ttgatgaacg
    18181 cgccaaaaag ctttaaacaa tgtgccgcac gcgcggggtt ttcaaagggt cctcttatgg
    18241 gtgaggatat atttaaagca atcatcgaaa atccacaggg cttgtggatt ggcaagtctg
    18301 atcctttaga caatttttcc gatttaagta ctgatgataa aaaattgcat atccatatac
    18361 ccgagatgga tgattggtgc aatgaggtca cggttgaaaa tgaaatcagg gaaattgaat
    18421 cccgaaaaga atatccattt attctatcag cgggaagaca ttatccgtat aatgcaaata
    18481 cattaatgcg aaatcccgcg tggaataagg ggaagagagt atgtacctgt attatgcacc
    18541 ctgatgatgc aaatgaactc aatatgaaag acggcgatat ggtcagggta ataactgaag
    18601 cgggttttgt tgaactggaa cttgaagtga ccgatcagac tgcgcgaaaa taccttatga
    18661 ttcctcatgg gtttgggctt gagtatggag gcaaaataac aggagttaat gtcaattctt
    18721 tggccagaaa tactcaccgc gacaggattg ccggcacccc gtttcaccgg tatatcccgt
    18781 gcagggtgga aaaagtttca taatcatttg ggcgatagtt atgtggatta cataaaagga
    18841 tacctcatga aaacaatctg cggatactca attgaaacat acatgtccat ggtagagaaa
    18901 tttcatggca gcatggcgcc aggaattatt atcggcggtt ttatggtgca tacactatgg
    18961 caacaccgca atcctgaaaa actgtacgat tttctatgtg aaacatctgt gtgcctccct
    19021 gatgcgatac aactgcttac tccctgcacg tacggcaacg gatggctcaa ggtggttcac
    19081 acaggccgct ttgccatggc tatgtttgat aagcttaccg gcgccggtag acgggcatgt
    19141 cttgacgcac agaaattgga accgtatcag gagattaaaa actggttttt caaattaaag
    19201 ccaaaaaaaa tgcaggataa agaccttctc attaatcaaa taattgcagc aggggaaaca
    19261 atactatcgg ttcaggatgt acagattgga catacctacc ttggaaaaga aaggttgggt
    19321 ccggtgggga tatgtccgga ttgcggcgaa gcatatccgc aaaaggacgg caaaagatgc
    19381 attgtttgtc aggggctgac cttatatgaa aagtaataga atacaatggc ttgccacaaa
    19441 actccaatct attttcaaag atattacatt tgatagaaat gagctctccg gagcatttgg
    19501 cgatattggt acagttattc cacttgtggt gggtgtttta ctggcaacag gaatggatgc
    19561 cggtaaggtg tttatggcct acggcctgat gcaaatcata actggtttgg tgtacaaaat
    19621 ccccatgccg gtacagccgc ttaaggtagt ggcgctgatt gtaattacac aaaaattaag
    19681 tgctgatata ctactctgtg gcgggcttgc cgttggcatc ttgatgcttg tattcactgt
    19741 aagcggattg attgatagtt ttacaaaaat aataccaaag gcagttatac ggggaattca
    19801 atttgggctg ggcttgcagc ttgtcatgct gtcattaaaa gagtatatcc cctcagaaag
    19861 attttcggat tatatccttg tggttgttgc atttttaatt gctgtcgctt tcatgggcaa
    19921 tagacgtata ccaccggcac tggttcttat acctgcgggg attttttacg gcatgatatt
    19981 caatacgggg gttcattatc agaaactatc gcacactatt actgttgttg atgccctgac
    20041 gtttgagaat atagtaaacg gcattgtgat acttgcatta ccgcaaattc cattatcact
    20101 ggggaattcg atttttgcaa ctagccagct cagccacgat ctattcccag aaaagagcgt
    20161 acaaccgcgt aaaattgctt tcacctactc attaatgaat attattaatc cttttattgg
    20221 tggcatagct gtatgtcatg ggagcggagg catggcgggc cattatgcat ttggcgggcg
    20281 aacaggtggg tcggtaatta tatttggtgc attgatgatt accaccggtt ttatttttgg
    20341 atcagatggt atttatattg cacgcttgtt cccgttaccc ctgctggggg ttattttatt
    20401 ttttgaagca cttacacttc tggtactcat tggtgatgtt atgcataaca gctttgatct
    20461 gaaaatagca ttggcagtag cagcattaat tgtggctatt ccctatggat atgtggttgg
    20521 gatgggcatt ggtatatgcg cctattacct tgctaaatgg ataaaagctg aaaagcttgc
    20581 atcagttaat ggtaaggcat aaaaaagaaa aggcataccg gtaaacaatg caactaccat
    20641 acgtaacacc tttatgagca catagccttg tgcatttgta atcataaaaa atgtattgca
    20701 tcaattattg gtatacagta ttattttaga tatacatatt acaaaagagg ctactatgcg
    20761 attacactat attatagtgg ttgtggcgtt tatattgtat aagctgtccg tagtttatgc
    20821 agttactctt tcctgggata ttccacaaaa tgaaaagctg gaatttgtgt atgatgccaa
    20881 tattgagtat tatgaaaatg gtatacttcg caaaatatat ggtgaacgta acatcattga
    20941 tttattgtgt tatgaaaagg acacaaaagg ccaggcagtc aggggctctt tttcagtata
    21001 cagaaaaaat attaatgatt atgtttaccg attggaagaa caatattttt ctgactttta
    21061 tattaacagc aatggtatat atgttgtgcc tgaaggcatt tttatgccta acttacggca
    21121 tctgccaaca ttcccggaga cggatgtgca tgttggagaa gtctggaaag ctcctgttaa
    21181 tatcgtattt aataattttt cagtccctct catattgctg cttgaagcac actatactct
    21241 gctatttata aatgaccaaa ctaaagaagc ctttatacaa tattccatac tcatagataa
    21301 aacattaaaa gacaaaccct atcctgagga tttgccaatt aaaatatatg ggcaatatgc
    21361 aggtcaggta acatgggact tacagaaaaa tcagccaaaa aaatcgatta atgcatataa
    21421 cctgctattg atttttggca gaaccgggga gttaagcact attgaattca agatgtctat
    21481 tgatcaaacc aataaggcat atctccatgt accagaagag gaaaagaaga agcagcaaaa
    21541 ggaaatagag aaagcattaa aacaggatgg tgtttctgtt gattctgatg ctcgtggtat
    21601 gattatacga ttttcggata ttctttttga ttttgacaaa tataacctga atgctgatgc
    21661 acgcaaagta ctggacaaac tggttgaaat aataaaagca aaatacccca acaatgagat
    21721 catcgtggaa gggcacactg acaatattgg tactgatgag tataaccaga atctttctga
    21781 aaagcgagcg cagactgtgg ctaccattct taaaaataaa ttagggcatg ataaagtatc
    21841 ctatcgtggc atggggaaga gcagacctat tgatgacaat tcaacggctg ccggaaggca
    21901 acgtaaccga agagtagaga tcattataaa gatgtaaatt attgcaaaat tctctgtgtt
    21961 ctacgtgtga atcgttttgc ttctgtctta aatgcctgag caagttctgc cagttcatca
    22021 aaccttttga caacaaataa tgattttaac aagtttttaa tctttgcatc atcatttatt
    22081 tcatgaatct gtttattgga tgcaatgaac gtttcgttgc agaagttaaa atttttttca
    22141 ataagctgac caaccggttc agatatcttt gcaaaaggca ttcctgtctt gagatgaaca
    22201 ttttttaata taagagcaat aagcgttttc atagtgcgct cttttgtggc ctgtatatcc
    22261 tgttgcattt tttcaaatgc tttcactatc atctggttcc tttctttaat taatctgatg
    22321 atttctcctt taatatcagg tggcattttg gaaaatgatg ggctgtgatg tggaaatgca
    22381 tatttcaggt atgctggttg aagcataata cgtgcacaca atgtgtcatt aaggttttct
    22441 ccctcaacca tttgtgcaat atcctcaggg gtaagtttta acagcccttt ttgttttatc
    22501 acatcaggta tgggcaattg gtagatgtat cctgcaatag ctttgttttc ggatgtttcc
    22561 tcagcaaaaa aatattcgca gagaagtctg gggaatccct tagcaggctc acgcgaatcg
    22621 ggatcttctt caatatcaat aattgcatca atcaggttat ggtacgtatt atgtgtttcc
    22681 atagtatata attgattagt atgtattcct taaaatatat ttgttactta aaaagggtaa
    22741 atactacagc gtaaagcaga gcatacagaa tttgatagtt taactaaaat gaataataca
    22801 atgatgtaat gaaaaaagca ataaaatttt taaggcctgg cttttacatc agttgataga
    22861 gggctgtata tacccttttt atagtactga tatcctatcc ctgcaaccat agcaccatta
    22921 tcagtgcata atatgggtga tgggaaaaat attgtatggt attccttttt tttctcaaaa
    22981 attagttcac gtaagcgttt attgctagca acaccacctg ctattaccag tgtgtgtatc
    23041 tttaaccgct tggaagcatc aaaaagcctg cgcactaata tttccagggc acgttcctga
    23101 aagctgtaag ctatttcgct tatgtcagca tcaggattat catggatata attaattact
    23161 gcggtcttaa ggccactgta tgaaaaagaa taggtgtcat tgctatcagc aagaattttt
    23221 gggaatatgg ttttttttaa cgttgcctgg cttgcaagct tttctataat tggcccacca
    23281 ggatatccta atgacaaaaa ttttgcaact ttatcaaatg cttctccaac cgcgtcatca
    23341 gttgtattgc ccaaaagctc aaaattcccg taatcatgca cacagtacag ggcagtatta
    23401 ccaccggaaa ccagtaaacc aataaaaggg aattcaatgc catgtccttc cagaaacgga
    23461 gcgtataaat gcgcttctaa atggtgtaca gctataaggg gtattgacag ggtatagcta
    23521 atagcctttg ctgattgcaa tgcaacaagc agtgaaccaa caagacctgg cctgtatgta
    23581 gcagcaacat atgaaagttc tttgaaagtg acctttgctt ctttaaggca atggtcaatt
    23641 aaatcattaa tgacctctaa atgggcacgt gatgcaatct caggtactac tccatagtat
    23701 tctttatgaa gatggatctg gctgaaaatt gcatgagata gtattgtttt tccatcagca
    23761 actatcgcta ctgctgtctc atcacatgaa ctttctagtc ctattcctaa aattttattg
    23821 ctcattaatt atttttattg cctcggttaa ctgattatca aattcaaggt catacaatgg
    23881 ccgttttgta tagcggtata tttctgattt taaaataaaa tatgccgttg aatcagaaag
    23941 cttaatggaa ttcttttcta gaagcttttt aaactcctgt tttgttggct catcatattt
    24001 agtgtgtgta cttataaatt tttccagtat cttttctttt atcagcatat ttatctgctt
    24061 ttgttcatct ttggagatag ttgccatagg aacaacgtaa tctggctgga ttcctatacc
    24121 gtgaattgaa ataccagaag gtgtgtaata tttagcgata gtcagcgtga caccagtatt
    24181 ttcatctaag ttaaagaatt tttgcactga acctttgcca aacgtttttt cccctaataa
    24241 tttggctcgg ttgttatcct ttagtgcacc ggatacaatc tctgaagccg aagcacttcc
    24301 tttattgaca agaataatgc atttaccggt gaataacgga tttgaagatg cacgatattc
    24361 ttgcaatgtt tccttcccgc ctctaccttt agttgataca atcatggtcc cttttggtaa
    24421 aaacatatcg cttatggtaa tagcctgatc caataaccca ccagggttcc atctgagatc
    24481 gatgatgcat tttttaatat tgttgttttg aaaatactgt aatgcctttt ttacctcggt
    24541 tgaggtatct tcactgaaga cttttactct gaggtatcca acaccagtgt tatcgattac
    24601 agcataatta accgtttgta actttattgg caatcgttca agttcaacat caatgggttc
    24661 atcaatacct tcacgaataa tagaaaggat gacttttgta tggggtttac cccgtatcat
    24721 ggaaacgata tcattaatgt ttttattctt tattggcttg ctatcaattt tataaataat
    24781 atcaccaggc tgaattcctg ctttcattgc aggagtacct tcgataggag atacaacaat
    24841 aatatcacca ttttttgcag aaatttcaat tcccacacca ataaactcac cactggtttc
    24901 ttcttttagc tggctgtatt cctgttcatc taaaaaacgt gaaaaagggt catttagtga
    24961 tgcaatcatg ccgcgaatag cgccataaaa aaggttcttg gagtcaggct gttctacata
    25021 ttcattacgg ataagctggt atacttgatg aaagaagtct aaatatttaa atgaaggttc
    25081 ttgcgcagaa acagtaaatg caatattaac gccaataaaa attcctgcaa gaaaggcaat
    25141 aaaagcaata tgaaatgaac gtttgcgtaa ccactgtatc atagtttcct cacttggttt
    25201 aagggtttac atcaaaaagt ttatatttat caggatgttt tgttttacag taactgtaaa
    25261 atgcctgctg gcttacttta aaatcatgta acactgtatc catgatttct cgtcgctgtt
    25321 ttgatgattg ctgttcatca aaactttcaa taaattcacg ggaaaggtat tcctgccata
    25381 aaattgaaaa ttcttcttcg gataactttt caccaataca ggctgtaaag tatcccagca
    25441 taatacacaa tataagggca aaaattttct tcattgttac aatcctggta tgtaaaatta
    25501 tacattaaat tttttaatca tttcacaaat ttactttgta ttgaaaatga aattcacttt
    25561 cctgtcgctt atgacaatgg aatggtacat attatcatat tgtgttcata ctattatttt
    25621 caagaattaa taatgcaaat tagttattat tataactata taaaattgta aagattttta
    25681 ttgtgtggtt taaataaaat cttaaatttt atagctctga ccccaatgca cttgtatact
    25741 ctacgttttg tactctacgt gatacgccta ataataatgc tctgacccca ttttgtaaaa
    25801 tttttattaa aa
//
LOCUS       DAORYL010000039        25795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1606_length_25795_cov_12.801709, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000039 DAORYL010000000
VERSION     DAORYL010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 25795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25795
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1606_length_25795_cov_12.801709"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLV81_08490"
     CDS             complement(<1..410)
                     /locus_tag="PLV81_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOM10176.1"
                     /translation="MKKDIDYFKRYPLLPLRDMVIFPHMVIPLFVGRDISIKALDSAM
                     KGDRLLVLAAQVDSQIANPKKEDIHDVGTLSEILQLLKLPDGTVKVLVEGISRVYISQ
                     FESTDDGITVGIKDIERPYETDSKVEALKRNLIDT"
     gene            complement(483..1751)
                     /gene="clpX"
                     /locus_tag="PLV81_08495"
     CDS             complement(483..1751)
                     /gene="clpX"
                     /locus_tag="PLV81_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOM10177.1"
                     /translation="MSNKKKDNGKPDITHYCSFCGKSQDLVRKLVAGPNVYICDECIE
                     LCNEILEEDWALEGGDLLTSNLPKPHEIKAILDEYVIGQEYAKKVLSVAVYNHYKRIT
                     SNFNLRKGDVELDKSNILLIGPTGSGKTLLAQTLAKILKVPFAIADATSLTEAGYVGE
                     DVENILLRLIQNADGDVKRAEIGIIYIDEIDKISRKSENPSITRDVSGEGVQQALLKI
                     VEGTIANVPPQGGRKHPHQEFISIDTKNILFICGGAFVGLDKIVHARIGNKTMGFGAE
                     VKSVKDIGKINILEKMIPEDLIRYGLIPEFVGRLHVHAVLHELDKQALREILTKPRNA
                     LTKQYQKIFAFENVELEFTDSAIDAIAQIAIERETGARGLRSVLEEIMLDLMYEIPSK
                     KTIAKVIINEDVVLYRKSPEIIQKSAEKSA"
     gene            complement(1788..2378)
                     /gene="clpP"
                     /locus_tag="PLV81_08500"
     CDS             complement(1788..2378)
                     /gene="clpP"
                     /locus_tag="PLV81_08500"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOM10178.1"
                     /translation="MSYLVPIVVEQTARGERAYDIYSRLLKDRIIFIGDAIDDHVASL
                     VIAQLLFLEAEDPDKDIYVYINSPGGIVTSGLAIYDTMQYVKPDVATICLGQAASMAS
                     LLLAAGAPGKRSALPNSRIMIHQPMGGFQGQASDIAIHAKEILKIKSKLNEIYAIHTK
                     QPIEKIEKDVDRDYFMDAQEAVEYGLIDKVIQKDSR"
     gene            complement(2474..3817)
                     /gene="tig"
                     /locus_tag="PLV81_08505"
     CDS             complement(2474..3817)
                     /gene="tig"
                     /locus_tag="PLV81_08505"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOM10179.1"
                     /translation="MEIKESKLENARIRLDIEIPNDRVDAEYEKVLKNVQKKAEIKGF
                     RVGKAPLDLVEKKYAEFVTGEAAENIVRSAYIEAIEQKGYTPVSQPVFDFKDLEKGKP
                     FSFIVEFDIPPTIELGNYKGIHVEQKTVKVTDEDVDAEIESMRERMATVTKKEGEDAR
                     VAKGDQVTIKMKRIDDKSSEEIDATAFTSHSLVVGKSKESWAIDDDIIGMKKGEEKEI
                     KVKYPKDYEVEDLKGQKVTYLVKVEEIFERQLPELNDEFAKSVGEYNSLEELKTRIRE
                     NLETYAKEQTLNQAKNDILTKIVENSKFDIPQSLIQKEMYAIFRRLQSRFGNVGKDIN
                     EFASIFGINPEELSKSIMDEAQRNIKNALVLTKIAELEQLKVSEEQFQKFIKSIAEQN
                     GVTEEEVVKVIEEKGNRDEIEGDLILDTAYDFIYQNADIKMLKPVTFQEYITQKK"
     gene            4107..5450
                     /locus_tag="PLV81_08510"
     CDS             4107..5450
                     /locus_tag="PLV81_08510"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HOM10180.1"
                     /translation="MDKTYYAKKYLIPINDSDIDWDNEFNQDEKNILRGINQRVVKGM
                     HLDEILDFVFDEASKILPLDRLDVAFLDDDNTRLVLYYTKTSYNDIHLLRGYSSDIQG
                     GSIQVVLNTGYPSIINDLASHLSSYPKSESAQLLLKEGIHSSIASPLKVDNRDVGIIM
                     FRSKQINAYTMHHIKLLLAFRDRLAQAVEKTYHIEKLTATINAYMEMLGFITHELKNP
                     LASIITLGRTIASGYFGEISDKNKEMVNRIVKKTEYLHNIALEYLNLARFESGVFNTK
                     FSEVNFFEEIVTSSIDFVETNLNEKKMKIILHNNAGNIKVLCDPELMKIVMNNLLSNA
                     IKYGYHNGQIILLVTADENIIHVSVKNDGPGFPESEKVKLFKKFSRLNTPELMDQSGH
                     GVGLYVTWKIIQMHSGHIWAHSEQGKWAEFTFEIPLRMDQCLIQPSRSFQDDVMR"
     gene            complement(5762..5833)
                     /locus_tag="PLV81_08515"
     tRNA            complement(5762..5833)
                     /locus_tag="PLV81_08515"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5799..5801),aa:Gly,seq:gcc)
     gene            complement(5838..5919)
                     /locus_tag="PLV81_08520"
     tRNA            complement(5838..5919)
                     /locus_tag="PLV81_08520"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5883..5885),aa:Leu,seq:tag)
     gene            complement(5988..6060)
                     /locus_tag="PLV81_08525"
     tRNA            complement(5988..6060)
                     /locus_tag="PLV81_08525"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6025..6027),aa:Lys,seq:ctt)
     gene            complement(6246..9752)
                     /locus_tag="PLV81_08530"
     CDS             complement(6246..9752)
                     /locus_tag="PLV81_08530"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOM10181.1"
                     /translation="MDTLFVLDILQFANCDFVIVDSSLHIIHSNNSSYINKNIKEVFP
                     YSIIDDVHNYFEKLTIDKPTIIIDVPDKHIPYSFSLHKIKFNKRDVLLIVPKFNQKIP
                     YIMKKIYEDLYQLQIFDEDADHFADVFNYILDTTLRVTGMDCGGIYIIDPLTQELKLT
                     FAKGFNATFLEKASRYTPDSWNYQLIMKGQPQYIDYTTMNLSDKDFRKVEGIKAVATI
                     PVLLHDRVIGAINIGSHSTNKIPETINPLLEAIGQQVGVIIDRFLIKSLMAHSQFDDV
                     KISPFHDIVYQVDKDGIIRYISSNIKNFIGFYPYELIGKSFTEFIFPEDLPMVLESYE
                     RVKKRIIEPLEYRLITKDGLYRYVLTISQSHYDEQGNFLGLSGVGTDIHEKKLSEIKL
                     IEREEKFKTLANSVLTAIFIIQDDILQWINPETAHILEYSEEEIIGKPFYYFVHPDDV
                     ESARLRADARQRGEDIINRYEARIITKNNIVKWIDFNVATIMYNGRIAILGSASDISD
                     RKKIELELKTSEEKFYKAFYSSPIPMTINTFRDGVYREVNEAAYQTFGYVPEESIGRS
                     IFELNIFADKEELQNYIHTLRTNEKVKDFHVTFKTKNGLLKDCIINSDVFVSSGEKLV
                     ISSIIDITQLKRSAQIIYEQYKELQKINNDLIKTKEELTLRNIQLFEEKERFNRVLES
                     INDAVIVTDRAAVILLVNNATCNLLNKEYDELIGKDIISILDLDCKQPVNFTFNTIIE
                     ILQQKSISNECILHVGNVSYNIELYILPLKDQMNSITGTIVVLRDITQRKIIEEHIAR
                     TSKLESVGLLAAGIAHDFNNILTSIVGNLSLIKTRMNASSELYQHINDAEKASFKAKE
                     LTQQLLTFAKGGAPVKKVTSLHTMLKDIVTFVLRGSGIESKFIIDDNLYNVEVDEGQI
                     SQVIQNLTINAREAMNDHGLLKVYALNVNEDDPIKAIIGNDKYIRISIEDNGPGIQNE
                     NIEKIFDPYYTTKPHGTGLGLTVVYSIIKQHGGYIFVDSQPGKGTCFDIYLKATEKKV
                     ETIDENGNEISFIGKGRVLVLEDDENIQFILRTMLEELGYEVDIACDGDEAYKKYLVA
                     KESDQHYSFAIMDLTIPGKKGGKDTITQIRKFDNEFKVIVSSGYSNDPVMSNYKDYGF
                     NGILPKPYRFEELKQIIAAISN"
     gene            complement(9756..10448)
                     /locus_tag="PLV81_08535"
     CDS             complement(9756..10448)
                     /locus_tag="PLV81_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOM10182.1"
                     /translation="MTEKQLEKYLTPTQCIDSDSPDVLDYARTIIGSETDAVKKAVKL
                     FYAVRDDIKYDPYSPFYKPEHYRGSFVLNRKRGYCVSKASLLCALGRACNIPSRVGFA
                     TVKNHIATKQLLETLGSNIFVYHGFTEFYLNGKWVKATPAFNKELCLLHKISPIDFNG
                     VDDAIFPEYNTEKKQYMEYIEYHGSFADIPVETILEAWKECYGKERVELWITLFEQDF
                     ASRKFETEEIIK"
     gene            complement(10576..11214)
                     /locus_tag="PLV81_08540"
     CDS             complement(10576..11214)
                     /locus_tag="PLV81_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10183.1"
                     /translation="MQNTFSEYDIFRYIPDELDFTIHNPYLQEILQYKDKHLDEYQYW
                     NLRYELIKKYAFAVPTFQLLSIIQELSPIIEIGAGNGYWAYMLKQVGVDVVAFDKYPP
                     DDAQYPFVNHSDNVWFDEQWYDVQKGDALEAGQYPERTLFLCWPQPQSTMALEALQAY
                     KYAGGHTVVFIGDMIACAENRFYDMLAECTLKLKKQIWGYKYFNEIIAVYYL"
     gene            complement(11235..11308)
                     /locus_tag="PLV81_08545"
     tRNA            complement(11235..11308)
                     /locus_tag="PLV81_08545"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11272..11274),aa:Arg,seq:tcg)
     assembly_gap    11312..11411
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11423..11494)
                     /locus_tag="PLV81_08550"
     tRNA            complement(11423..11494)
                     /locus_tag="PLV81_08550"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11460..11462),aa:His,seq:gtg)
     gene            complement(11607..12641)
                     /locus_tag="PLV81_08555"
     CDS             complement(11607..12641)
                     /locus_tag="PLV81_08555"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOM10184.1"
                     /translation="MLYRIWTIFIARTKEFYRDRSALGWNIIFPLLIIIGFSVVFRSE
                     SSPVLKVGVVKPLNNEITIISNKQFNEFLKLKYIDFIEFSSKEKAVDSLQKFKIDMLV
                     DPEHNTYFINDSSPNGYIAEQLLKGAGTTTTFFSKHSFKGRGIRYVEWLFPGILGMNA
                     MFNSLYGVGYVLVMYRKNGILKRFSVAPVRPFEFLTAQILSRMFVMLATTIVVYIGTV
                     LLYGFQCKGSLFTLFVVFALGGFCMISLGLLIACRSDSQEFADGLINIITWPMMFLSE
                     VWFSLEGARPWVQKVSQLLPLTPIVESARMVMNEGATLFDIRYQLLYLIVVSAIFLLS
                     GSLLFRWQRK"
     gene            complement(12644..13528)
                     /locus_tag="PLV81_08560"
     CDS             complement(12644..13528)
                     /locus_tag="PLV81_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOM10185.1"
                     /translation="MDYILEVKNLYKYYPGVKAVDDVSFAIPRGICFGLLGPNGAGKT
                     TTIEVIEGIQKPDNGTILYKGKPRDQHFKQEVGIQLQHTELQQYLTVKEILLTFRNLY
                     ARRASLDELVSLCYLEEILDRDNRKISGGQKQRLLLAIALANDPELLFLDEPTTGLDP
                     QARRHVWDIVQSIKKKGKTIILTTHYMEEAQTLCDIVAIMDRGKIIAMDSPINLLNEH
                     CRGVTITLPSTVDENILRQMNCEWYTVNHTIEIHTEQPHDCLRLLIDNNVDITGMVVR
                     SQNLEDLFLKLTGTQLRA"
     gene            complement(13601..14956)
                     /locus_tag="PLV81_08565"
     CDS             complement(13601..14956)
                     /locus_tag="PLV81_08565"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOM10186.1"
                     /translation="MSINSAAFSNGETCFYHSDKIIITMSIKANKNQIKAINAENGAH
                     LVIASAGSGKTYTIIQRSIALINKGLCKPGELLLLTFSRKAADELKKRIIAGLGNCGN
                     DIVASTFHAFCLQYIIKQNMKQVTVLDEESSEKIIKQIVEKHVETFNGIPSSVMYKLA
                     LKNIQSINLPHDIYAAIKTIKDEYEQYKQVHNVIDFEDMINKSIDCLSKNISLRNTIH
                     KTFKYVMVDEFQDTSENNFKLLKLLLPEENPNIFMVGDDWQSIYKFRDAKIEYIVNAR
                     KYFDNLTVHSLNSNYRSKKEIVKLSNRLIAKNRFRSRRWVKSVRGGGGKIYFVKVDSF
                     EIEALIADTIAKQYDKKISIGVLYRNNWQGTFLQSRINTDDNVKFMTIHGAKGLEFDV
                     VILCGVKDRLLPDPYTDIEEERRLMYVALTRAKNCLHILYHPAYSNPKPQFIEECESY
                     V"
     gene            complement(15117..16742)
                     /locus_tag="PLV81_08570"
     CDS             complement(15117..16742)
                     /locus_tag="PLV81_08570"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOM10187.1"
                     /translation="MKIVLGYCVEKEHSHDYYITLLPVGLVSIAAYLSQKGYDVTLAN
                     FSKKSPEQIVKEIKTIKPHIIGFSLYTHNRVTTFSVIQKIKKVFPHSVVVLGGPHATF
                     LSQEIIKRYPYIDYVVEGEGEGAMLSIADAIKKGKPIKEKILKQNNIHIDTLPYPAKF
                     AGNLSGVNINEQFKYIITSRGCSFNCTFCDSPSFWGRKVRFRNIDDVIDELNLLYKKY
                     GIIYFSIRDDNFTIKKDRVQELSDKIIKKKLYMMWNCQGRVDSVDESMLIAMKKAGLE
                     HIQYGVESGSEKILSLYNKKITVEKILNAAAITRRVGIYLSVYLMVGMKGETYSDIKK
                     TKKLIATMLPGDCVVSPVALYPGTKLYEEEKQRGVLDDSIWFTKKDAGIYLRNEPMIQ
                     EWMNDLLLQTYNLRNKAWYMARDFALHRNVAGDCWVTDILEGDYYLDNEMYPEASHVY
                     AKVMNAMPDNPWGYMRYGKLHFAMGDFETAMESFFEVTKIVPNYYGGWLKYAQASLAL
                     GIKDSARAAISKAMELNKYDERIRSLYTLLHKK"
     gene            17031..17231
                     /locus_tag="PLV81_08575"
     CDS             17031..17231
                     /locus_tag="PLV81_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10188.1"
                     /translation="MQITISKNELYTMIKKAVKEVIYEERLDILLKSIPAISDEEMTE
                     IKNLYGHKPPKRKAAKRITIDI"
     gene            complement(17515..18297)
                     /locus_tag="PLV81_08580"
     CDS             complement(17515..18297)
                     /locus_tag="PLV81_08580"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HOM10189.1"
                     /translation="MKEYTVTIRFYEELNDFLPKDKKKVAFTVSWEGKRSIKDLIESL
                     GVPHVEVDLIQVNGISVGFDYIVQNDDYISVYPVFERFEISNITRLRPQSLRAPAFVC
                     DVHLRKLVKYLRLFGFDVLYNETWDDKDLAHIAAEQKRILLTRDRQLLMRKIVDRGLI
                     VRNTSPQKQIVEILDRLDLWDKINPFSRCIMCNGNVTELSNNTEEFEKEKNRIPPGVL
                     SWCNTYYKCTQCGRIYWEGSHYEKLTKKIETIINQKSVDKDS"
     gene            complement(18294..19202)
                     /locus_tag="PLV81_08585"
     CDS             complement(18294..19202)
                     /locus_tag="PLV81_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOM10190.1"
                     /translation="MDNGKHYEAIVAKKVTWTGMIVNIILSGLKFVAGILGHSQAMVA
                     DAIHSISDLTTDIAVLVGIKYWASPADDDHPYGHERIESIVTAFIALALFAVGIGIAY
                     NGIITAFEPDSKGPEAIALIAAFISILSKEWLYRWTVKKGKEIDSSSVIANAWHHRSD
                     ALSSIPALVAVALAIAHPSLVFFDHIGAVVVAMFIVKVAFDILKPVVMELSDRGASKS
                     QVDAIDLIAMKVDGVKEVHSIRSRRVGSGYFVDLHVLVDGNMSVQDGHDIARSVKHEL
                     LDKGPKVLDVIVHLEPYQTSSKKNNS"
     gene            complement(19195..20688)
                     /locus_tag="PLV81_08590"
     CDS             complement(19195..20688)
                     /locus_tag="PLV81_08590"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOM10191.1"
                     /translation="MKSKHWPIYRAIGQLLLWFVFIASIIYLHDPVSDAGIGFYPSLS
                     IHSITIGYITTHTVKYALLGLVLFILTFLLGRFFCGWMCPLGATIDASDTVIQPKGKT
                     KVQTNLSRIKFLVLLSSMLIAFFGFTVSGYIDPITIAYRAYELFLYPFVSFIGSKPLS
                     WILPGYQARDYPFYYYNWAWVAVVFFVVLGLSAFFRRFWCRVLCPLGAMYAIASHVSL
                     FRRVVDTDKCIHCGACVKGCRMGAIATDGVGTIEHECIKCFDCLKNCKYDAISFTFTR
                     PSKNKSIEYPGKGITRRDLLIATGSSVIVAASKVSLFAASHDSFIIRPPGALRESQFL
                     DACIRCGQCMNVCPTNALHPCITEAGFYGMFTPRLISRIGYCDFTCTRCGQVCPTGAI
                     QKLSLNSKQKFVIGTAVILHDICLPWSEQTNCIVCEEVCPVANKAITLEKKIVKNKKG
                     LQIEVLLPVVHEDRCIGCGICENRCPVSGQAAIVVRKPKLSIGERYG"
     gene            complement(20685..21608)
                     /locus_tag="PLV81_08595"
     CDS             complement(20685..21608)
                     /locus_tag="PLV81_08595"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOM10192.1"
                     /translation="MKITRKNLFSSIISATGFLAGNYLTGLKKAFGLEKKTDLVVIKT
                     NALTDKSIYGMVVKGFEEMGGIQKFVKKGSKVVIKPNMGFNSTPDRAHTTNPILVEAV
                     AAMCKRAGANVAIMDRPVHNARLCYRSSGIEEVAKKVGVDYVYMDRAKFKTVKVPGGL
                     NLDTLDVYEDILEADCIINMPIAKHHSAAQLTLAMKNLMGVIGGNRGYYHINLHRNIV
                     DFNKAVKVHLIILDGLRILTKHGPSSGSPADIRETKTVIFGTNPVMVDAYAAKQLFGI
                     EPSSIGYLNLAAQSGMGSINITAYSTITRTV"
     gene            complement(21611..22060)
                     /locus_tag="PLV81_08600"
     CDS             complement(21611..22060)
                     /locus_tag="PLV81_08600"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HOM10193.1"
                     /translation="MSEKKKIVIFLFVFGIIITLAGIKLIYNAQESLHWPKTEGVITQ
                     SFMDWDRERQHHANIKYEFIVNGEKITGFQISAKNVNKSSEELLREYPKGKKVTVYYD
                     PDDPVNSLLEPGYSWQSYQAFVLGILIILAGIGVLLFYKEKPQSAKG"
     gene            complement(22286..24685)
                     /locus_tag="PLV81_08605"
     CDS             complement(22286..24685)
                     /locus_tag="PLV81_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HOM10194.1"
                     /translation="MIIQKLTLKNFRQYKDATIEFKEGLIGIIGKNGSGKSTIFNAIV
                     CALYGVLPTAKEYMVSVFAEEKASVSIELVFQIDNTQYRIVREFRGKNLNQYASLYKN
                     DVAIATGATDVNRTIEDILGMPQDAFTHSVFAAQGELRQISETGGSDRRELIRKIMGF
                     SLLDEIVQMIRNDRNDKKRFIEGQQAMLLSDDDIKAKESALEKQYQELSHKEKEVQTL
                     QKNHELISEKFINVQKNFKEQQELYIKHNDLLKKQTQLQAQFDNCMQNIAEAKERLKK
                     LEHAKAEYEKLYGHEKKYLECKKKKEELEQARAMNMKKQALQKQYEELMHELHEKEKS
                     IKAEQSELQKLDDLETTIKQNDSRKKHIEQTVKSLQEELYTIKQHIGGIEKAVKDRKK
                     HIEQIEKLGREAECPVCLRPLHEAYDATIAKLLSEIEEYEKKELALLKSAAEKKDSML
                     KNADHEVKELDIRLNDLQQQKAQLLEKKKNVEKELSELKKKRDNLDVIGLQLQELSTV
                     MFDEQEYANIVEEYSKLEAFHTMFIGLEAQIREIPVLQQRIEQLEKQKNDRAVEIKQI
                     TEDIATLGFSEKLYHEVKNDYDSVLQEKEKIIKQLNEEKLLYNDIKNAIGKIDDELKK
                     DKQNRKKIDVAKQEFELLQRLDKIMDGFRESVLQMAMPVIANYASNLFNQLTQGRYQA
                     IEMDDTFNFQIMDDGRWYPLSRFSGGEIDCANLCLRIAISNAIRDFSGSGAVGFMAFD
                     EIFGSQDNERRNAIINALYTLQEQYRQIFIISHIDDVKELFPSILYVQTTANGSLAKW
                     L"
     gene            complement(24689..>25795)
                     /locus_tag="PLV81_08610"
     CDS             complement(24689..>25795)
                     /locus_tag="PLV81_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HOM10195.1"
                     /translation="THLGFNDFDKVNDDGINQREADVYNAFANAVDSIIQYKPDFVIH
                     TGDLFHRSSPSNRALIQGLQHIKRITQAGIPFIVIAGNHCTPKTIYTSPILQALQTID
                     GVYAVYKGEYECFSLDGIHLHCLPHIHEDAEFITQIKQIEPRQGHNILLMHTSIGKDY
                     LLDEYGERIFPEEYFSLLKEFDYVALGHWHNFNILPRWDNVCYSGSTERFSDREAGKQ
                     KGFVTVTIGTKTNIEFNPITIRDWVRFDIKTCSEYSVEDIKKNIQELSSQVAQGAIVS
                     VYLHDIDAHQSVAVSNMWIAQQFPQALVVLPKRIFKQQRSDSSSYKEHISIEEVFAAY
                     CKQSISDEKEREAVEALGKHYFIKYREQFSDIKE"
BASE COUNT         7935 a         5141 c         3912 g         8707 t
ORIGIN      
        1 gtatcaatta aattgcgctt taaggcttct acctttgaat cagtttcata tggacgctca
       61 atatccttaa tccctactgt aataccatca tcagttgatt caaattgtga aatatacaca
      121 cgtgaaatac cctcaaccag cactttaaca gtaccgtcag gtaattttaa aagctgaaga
      181 atctcggaaa gggttccaac atcatgaata tcttctttct ttgggtttgc tatctgtgaa
      241 tcaacctgag cagcaaggac caatagacgg tcacccttca ttgccgaatc cagcgcttta
      301 attgagatat cccggccaac aaatagtggt attaccatat gtgggaatat taccatatcc
      361 cgtaatggca ataaggggta tcgtttaaag taatctatat ctttcttcat agtttctcct
      421 gaattgtgtg agtagtgttc caatacataa aatactgaaa atagtgcaat ccggacacat
      481 acttacgcac ttttttctgc cgatttctga ataatttcag gggattttct gtacagtaca
      541 acatcctcgt taatgataac cttagctatg gttttcttgg aaggaatctc atacatcaga
      601 tccagcatta tctcttcaag aactgaccgc aatccacgtg cgccggtttc gcgttctatt
      661 gcaatctgag ctattgcatc aatggctgaa tcagtaaatt caagttcaac attttcaaag
      721 gcaaatattt tctggtattg cttagttaaa gcattgcgcg gttttgttaa aatttccctt
      781 aaagcctgtt tgtcaagctc atgcaataca gcatggacat gcaatctgcc tacaaactct
      841 ggaattaaac cataccgtat aagatcttca gggatcattt tctccagtat attgattttg
      901 ccaatatctt tcactgactt aacttccgca ccaaatccca tggttttatt accaatgcga
      961 gcatgaacaa ttttatccag cccaacaaat gcgccaccac aaataaacaa aatgttcttg
     1021 gtatcaatgg atatgaactc ctgatgagga tgttttctgc caccctgtgg tggaacattg
     1081 gcaatagttc cttcaacaat ctttaataag gcctgctgta caccttcacc agatacatca
     1141 cgtgtaatag atgggttttc agatttacgt gaaatcttat cgatttcatc gatataaatt
     1201 attcctatct cagccctttt aacatcgcca tctgcatttt gaatcagtct cagcaatata
     1261 ttttctacat cctcacccac atagcctgct tcggtcagtg atgttgcatc tgctattgca
     1321 aacggtactt ttaaaatttt tgccagtgtt tgtgcaagca atgttttacc gcttcccgtt
     1381 gggccaatta ataaaatatt gcttttatct aattcaacgt cgcctttgcg taaattaaag
     1441 ttactggtga ttcttttata gtggttatag actgcaaccg aaagtacttt ctttgcatat
     1501 tcctgcccaa taacatattc atcaagaata gctttaatct catgaggttt tggtaaattg
     1561 ctggttaaaa ggtcgccgcc ttctaatgcc cagtcttctt ccagtatctc attacataat
     1621 tctatacatt catcacagat atatacatta ggaccagcaa ctaattttcg taccaggtcc
     1681 tggcttttcc cacaaaatga acaataatgg gttatgtcag gcttgccatt atcttttttc
     1741 ttatttgaca tatatcctct taataataat tatctcttat acattactta tctactatct
     1801 ttttgaataa ctttatcaat taatccatac tctacggctt cctgggcatc cataaaataa
     1861 tccctgtcca catctttttc aattttttct ataggctgtt tggtatggat ggcatatatt
     1921 tcattcaact ttgacttaat tttcaggatt tccttggcat gaatagcaat gtcagaggcc
     1981 tgcccctgaa agccacccat tggctgatga atcattatac gcgaatttgg cagtgcagaa
     2041 cgttttcctg gtgcacccgc tgccagtagc agtgaagcca ttgaagctgc ctgccctaag
     2101 catatagttg caacgtcagg cttaacgtac tgcatggtat cataaatagc cagaccagat
     2161 gttacaatgc ctcctggtga gttaatatat acataaatat ccttgtcagg gtcttctgct
     2221 tccagaaata acaactgagc tatgactaaa cttgctacat ggtcatcaat tgcatctcca
     2281 atgaaaatga tacgatcctt taataaacgg gaatatatat catatgcccg ctcaccacgt
     2341 gctgtttgtt ctacaacaat tggtacaaga tagctcatga aattacctca acaaatttat
     2401 aaatttctaa ataaccatat atatagtgta gcatctgttc ccgtaacttc tataatttgt
     2461 tatgaatgtc tattcacttc ttctgagtaa tatattcctg aaaagttaca ggcttaagca
     2521 tcttaatatc tgcattctga taaataaaat catatgctgt atccagtatt aaatcacctt
     2581 caatttcatc cctgttccct ttttcctcaa tcacttttac gacttcttct tctgtaacgc
     2641 cattttgttc agctattgat ttgatgaatt tctggaactg ttcttcagaa actttaagct
     2701 gctcaagttc ggcaatcttt gttaacacca gtgcattttt aatattgcgc tgagcttcat
     2761 ccataataga tttcgatagc tcttcaggat taatcccaaa aattgaagca aactcattga
     2821 tatctttgcc aacattgcca aatcgtgact gcaatctcct gaaaatagca tacatctctt
     2881 tttgtatcaa ggattggggt atgtcaaatt tactgttttc aacaatcttt gtcagtatat
     2941 cattctttgc ctgattcagt gtctgctctt ttgcataggt ttccagattt tccctgattc
     3001 gggtcttcaa ctcctcaaga gaattgtatt ctcccacact tttagcaaat tcatcattga
     3061 gttcaggtaa ctgtcgctca aaaatctctt caaccttcac cagatacgtt accttttgcc
     3121 ccttgagatc ttccacttca taatctttgg ggtatttaac tttaatttct ttctcttcac
     3181 cttttttcat cccaataata tcatcatcaa tagcccatga ttctttgctc ttgccaacaa
     3241 caaggctgtg cgaggtaaag gcagttgcat ctatctcttc ggatgatttg tcatcaatgc
     3301 gcttcatttt aattgttacc tgatcacctt tagctaccct ggcatcttca ccttcttttt
     3361 tggttaccgt tgccatccgt tcacgcattg attctatttc agcatcaaca tcttcatcgg
     3421 ttacttttac tgttttttgc tcaacatgaa tgcctttata attgccaagc tcaatagttg
     3481 gcggtatatc aaattctact ataaaagaaa aaggctttcc tttttcaagg tctttaaaat
     3541 caaatacagg ctggcttact ggcgtatagc ccttctgttc aatggcttca atatacgcag
     3601 accgcacaat attttcggca gcttcacctg taacaaattc agcatatttt ttttcaacta
     3661 aatcaagcgg cgctttccca accctgaagc ctttaatttc agccttcttc tgaacatttt
     3721 ttaaaacctt ttcatattca gcatcaacac ggtcattagg tatttcaata tcaagacgta
     3781 tccgtgcatt ttcaagtttt gactctttaa tttccaaggt tgactctcct cattatatat
     3841 taaaatatta tatagtaaat tcattaccag ataaaatgaa gatgggatac ttttatgtta
     3901 atcatacagg gctgatacat caatttttca tggaaagacc atgaaatata tgaataatgt
     3961 actattatag cacaattttg agttaactat agaataaacg attgattttt tgtatattta
     4021 attttaccat acataccgtc aaatactttt ttaagcgata gttattaata tattagcctt
     4081 ttaatagatt aaggaatgaa acatttatgg ataaaaccta ttatgcaaaa aaatacctga
     4141 ttccaataaa cgacagcgac atagattggg ataatgaatt taaccaggac gagaagaata
     4201 ttttgcgtgg tattaatcaa agagttgtaa aaggcatgca tttagatgaa attctcgatt
     4261 ttgtttttga tgaagcatcg aaaatattac cactggacag actggatgtt gcttttcttg
     4321 atgatgataa cacgcgactt gtattgtact atacaaaaac ctcttacaat gacatacatc
     4381 ttctcagggg atattcttct gatattcagg gaggatctat ccaggtagta ttaaatacag
     4441 gttatccaag tattattaat gaccttgcat cacatttatc aagctatcca aaaagtgaat
     4501 cagctcaact tctattaaag gaagggattc attcatcaat tgcttcacca cttaaagtag
     4561 acaaccggga tgtaggtatt atcatgttca ggagtaagca aattaatgcc tataccatgc
     4621 atcacatcaa attgctatta gccttcaggg atcgcttagc tcaggcagta gaaaagacgt
     4681 accatataga aaaattaact gcaacgatca acgcatacat ggaaatgctg ggttttatta
     4741 ctcacgagtt aaaaaatcca ttagcttcaa ttataactct gggaagaaca atagcatcgg
     4801 ggtactttgg tgaaataagt gataaaaata aagaaatggt aaacaggatt gtaaagaaaa
     4861 ctgaatattt acacaatatt gcacttgaat accttaattt agcgcgtttt gaatctggtg
     4921 tttttaatac aaaattttcc gaagtcaatt tttttgaaga gatagttaca tcatcaatag
     4981 attttgtaga aaccaacctg aatgaaaaaa agatgaaaat tattttacac aataatgctg
     5041 ggaatataaa agtattatgt gacccagaac tcatgaaaat tgtcatgaat aatttattaa
     5101 gtaatgcaat aaagtatgga taccataatg gacagattat attgcttgta actgctgatg
     5161 aaaatattat tcatgtaagt gtaaagaatg acggacctgg attcccagaa agtgagaaag
     5221 taaaactttt taaaaaattt tcaagactaa atacacctga attaatggat cagagtggcc
     5281 atggcgttgg cctttatgta acatggaaaa tcattcaaat gcattcgggc catatctggg
     5341 cacattcaga acaaggcaaa tgggctgaat ttacttttga aataccattg cgtatggatc
     5401 aatgcctcat acaaccttca cgctcatttc aggatgatgt aatgagatag tatgatgtta
     5461 ataaattagt aatatactga attctattga gaattccata attcagattt ctcctcactt
     5521 tcacaacctc aataacctca gataaataat cccattgatc tcgcttcgat aaattcaaca
     5581 acctcgatgt agagaagaat cctgttaatt aaaaatttag attctttcct atgctcagta
     5641 tatcaattat tgcctcattt ttatcaattt gggtaagtgc aatcgaagtc taggattgat
     5701 ctatataatt atgatagaat aaaaaaaagc tgcttttaat taagcagcct tttaaaaaat
     5761 gagcgggaaa cgggatttga acccgcgacc tcaaccttgg caaggttgca ctctaccgct
     5821 gagttattcc cgcctattgc gagcgaaggg acttgaaccc ccacacccta aggtactaga
     5881 tcctaagtct agcgcgtcta ccagttccgc cacgctcgca tactaaaata caatatcaaa
     5941 aaatcaagta attttttcaa gctaaattta tattgcgcag cggaatttgg gttgcccgag
     6001 actcgaactc ggaaccatct gattaagagt caggtgctct accagttgag ctagcaaccc
     6061 ctctttcaaa tacttaaacc tgactttagt taatcaaaaa ataatttaaa ataaagattt
     6121 tttcaatatt aaattatgta attatgtgtc aagttttttt taaagcaatt ataaaaataa
     6181 taggttaaaa tagtaattat tataaaaaat agctctttag ctaaaatttc taaaaaaatt
     6241 tcatgttaat tacttattgc agcaataatt tgttttaatt cttcaaaacg atatggtttg
     6301 ggtaatatcc cattaaagcc ataatcttta taattagaca taacaggatc atttgaatac
     6361 ccactggaaa ctattacttt aaattcgttg tcaaatttgc gaatctgtgt tatagtatct
     6421 ttaccacctt ttttacctgg tatagttaaa tccataatgg caaatgaata atgctggtct
     6481 gattcttttg ccactaaata ttttttatag gcttcatcgc catcacaggc tatatccact
     6541 tcataaccca attcctctaa catcgtgcgt aaaataaact gaatattttc atcatcctcc
     6601 agaaccagta ctctaccttt tccaataaat gagatttcat taccattttc atctatcgtt
     6661 tcaacttttt tttcagttgc tttaagatat atatcaaaac atgtgccttt acctggttgt
     6721 gaatcaacaa atatatagcc accatgttgt ttaatgattg aataaactac cgtcaatccc
     6781 aggccagtgc catgtggttt agtagtgtaa tatgggtcaa aaatcttttc aatattttca
     6841 ttttgtattc ccgggccgtt atcctcaatt gaaattctta tatatttatc attaccaata
     6901 atagctttaa tgggatcatc ttcattaaca ttcaatgcat aaactttcaa cagaccatga
     6961 tcattcatag cttcccgagc attaattgtt agattttgaa taacctggct gatttgccct
     7021 tcatccacct ctacattata gagattatca tctatgataa acttagattc tataccagaa
     7081 ccacgcaata caaaagtaac tatgtctttt aacatagtat gtaaagaagt aaccttcttt
     7141 accggagcac ctccttttgc aaacgtcagt aattgctgcg tgagctcttt tgctttaaat
     7201 gacgcttttt cagcatcatt aatatgctga tacaattctg atgacgcatt cattctagtt
     7261 ttaattaaag aaagattacc tacaattgag gtaagtatat tattaaaatc atgagcaatg
     7321 cctgctgcca gaagcccaac tgattctaat tttgaagtcc gtgcaatatg ctcctctatt
     7381 attttccttt gtgttatatc gcgtaaaaca acgattgttc ctgtaatact attcatctgg
     7441 tcttttaatg gcaggatata taattcaatg ttataggaaa cattaccaac atgcagaata
     7501 cattcatttg agattgattt ttgctgtaat atttcaatta tagtattaaa agtaaaatta
     7561 actggttgct tacaatccaa atcaaggata gatataatat cttttcctat gagttcatcg
     7621 tattctttat tcaggagatt acaggttgca ttatttacca gtagtattac cgctgctcta
     7681 tcagttacta taacagcatc attaatggac tcaagtaccc ggttaaaacg ttctttttct
     7741 tcaaataact ggatattgcg aagtgttagt tcttcttttg tttttattaa atcattatta
     7801 attttttgta attctttata ctgttcatat ataatctgcg cacttcgttt caactgagta
     7861 atatcaataa ttgagctaat gactaacttt tcccctgaag aaacaaaaac atcagagtta
     7921 ataatacaat cttttaataa tccattcttt gttttaaacg ttacatggaa atctttaact
     7981 ttttcatttg tccttagagt atgaatataa ttctgcaact cttctttatc agcaaatata
     8041 tttaattcaa atattgacct gccaatggat tcctcaggca catagccaaa tgtttggtat
     8101 gctgcttcgt taacttctcg gtaaactcca tctctgaatg tatttatagt cattggaatg
     8161 gggcttgaat aaaatgcttt ataaaacttt tcttcagaag tttttaattc caattcaatt
     8221 tttttgcggt cgcttatatc tgaagctgag cctaaaatgg caatacggcc attatacatt
     8281 attgtagcta cattaaaatc aatccacttt actatattat tttttgttat tattcttgcc
     8341 tcgtacctgt ttattatatc ttcaccgcgc tggcgagcat ctgcacgtaa tctggcagac
     8401 tcaacatcat cgggatgaac aaaataataa aaaggcttgc caataatttc ttcttcagag
     8461 tattcaagga tatgtgcagt ttctggatta atccactgca aaatgtcatc ctgaataata
     8521 aatattgcag ttaatacaga atttgccaat gttttaaatt tttcttctct ttctattagt
     8581 tttatttcag ataatttttt ttcatgaata tcagtaccaa cacctgaaag gcctaaaaaa
     8641 ttaccctgct catcataatg tgattgtgat attgtaagaa catacctgta taaaccatct
     8701 tttgtaatta agcggtattc caatggttca ataattctct tttttaccct ttcataactt
     8761 tccaatacca ttggtaaatc ttcaggaaat ataaattcag tgaaagattt accaatcaat
     8821 tcataagggt aaaacccaat aaaatttttt atgtttgaac ttatataccg aataatgcca
     8881 tctttatcaa cctgatatac tatatcatga aacgggctta ttttgacatc atcaaattgt
     8941 gaatgtgcca ttaaactttt aattaaaaat ctatctataa taacaccaac ctgttgccca
     9001 atagcttcaa gtaaagggtt aatggtttca ggaattttat tagtagaatg tgaaccaatg
     9061 ttaatagcac ctataacacg atcatgtaat agtaccggaa ttgttgctac agcctttatt
     9121 ccttcaacct ttctgaagtc tttatcagat aaattcattg tagtgtaatc aatatactgt
     9181 ggttgcccct tcattataag ctgatagttc cagctatcgg gtgtatatcg ggaagctttc
     9241 tctaaaaaag ttgcattaaa tccttttgca aaggtcaact taagttcctg tgttagcgga
     9301 tcaatgatgt atataccacc acaatccatt cccgtaactc ttagtgttgt atctaaaata
     9361 tagttaaata catcagcaaa atggtcggca tcttcatcaa agatttgtaa ctgatacaaa
     9421 tcttcatata tttttttcat tatataagga atcttctggt taaatttagg tacaattaac
     9481 agtacatcac gtttattaaa ctttatctta tgtagtgaga aactataagg aatgtgttta
     9541 tcgggaacat cgattattat tgttggttta tcgatcgtca acttctcaaa gtaattgtgt
     9601 acatcatcaa ttatcgaata tggaaatact tcttttatat ttttattaat atatgatgaa
     9661 ttattagaat ggattatgtg taatgaacta tcgacaataa caaaatcaca attggcaaat
     9721 tgcaaaatat ctaaaacaaa caatgtgtcc ataggctatt ttataatttc ttccgtttca
     9781 aatttcctgc tggcaaaatc ctgttcaaaa agcgtaatcc acaactcaac tcgttcttta
     9841 ccgtaacact ccttccatgc ttcaagtatc gtttcaacag gtatatccgc aaaactgccg
     9901 tgatactcta tatattccat atattgcttc ttttcagtgt tatactcagg gaaaattgca
     9961 tcatccacac cattaaaatc aataggggat attttatgga gcaggcataa ttctttattg
    10021 aaggctggtg ttgcttttac ccattttccg tttaaataaa actcagtaaa accatgatac
    10081 acaaatatat tacttcccaa tgtttccaga agttgctttg ttgctatatg atttttaaca
    10141 gttgcaaagc ctactctgct agggatattg caggcacgtc ctaaagcaca taataaagag
    10201 gcttttgata cacagtatcc acgcttgcgg ttcaatacaa aactgcctct ataatgttca
    10261 ggcttataaa acggagaata cggatcatat ttgatatcat cacggacagc ataaaaaagc
    10321 tttactgcct ttttaacagc atcggtttca ctgccaataa tagtgcgagc gtagtcaaga
    10381 acatcagggc tatcgctatc aatgcattgt gttggcgtaa gatatttttc caattgcttt
    10441 tcagtcatat aagtagtatg tggttataaa tttaggtaat agtataaata agtatagtcc
    10501 aaatcatgtt tccttcaata attattgtca atcctaaata ctataaatgt aatgtcaaag
    10561 aaatagtatc ttggattata gataatacac tgcaataatt tcattaaaat acttatatcc
    10621 ccaaatctgt tttttcaact ttaacgtaca ctctgcaagc atatcataaa acctgttttc
    10681 agcacaggca atcatatccc caataaaaac tacagtatgc cccccagcgt atttataagc
    10741 ctgtaatgcc tctaaagcca tggtactttg tggctgaggc cagcataaaa agagtgtccg
    10801 ttctggatac tgtcctgctt ccagtgcatc acctttttga acatcgtacc attgttcatc
    10861 aaaccacaca ttatctgagt gattaacaaa cggatattga gcatcatctg ggggatattt
    10921 atcaaatgca accacatcaa cacccacctg cttcagcata taagcccagt atccattgcc
    10981 tgcacctatt tcaattatgg gcgatagttc ctgtataata gacagtaact gaaaggttgg
    11041 tactgcaaat gcatactttt ttatcagttc atagcgcaag ttccagtact ggtattcatc
    11101 caggtgctta tctttatact gtagtatttc ctgtaaataa gggttatgga tagtgaagtc
    11161 taattcatcg ggaatatagc ggaatatatc atattcactg aaagtgtttt gcatggtgaa
    11221 tatactgtat ggtgcgcgcc cggcaggagt cgaacctgcg gcctgcggat tcgaagtccg
    11281 acgctctatc cagctgagct acgggcgcga gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn naaataaaaa tgggtggatg acggggctcg aacccgcaac aactggatcc
    11461 acagtccagg gctctaccat tgagctacat ccaccgtaac tatctgacaa ataaactata
    11521 attaggaatg acaatactta aaatgtaatt atagtgtcaa ttatatttca actcaaaatt
    11581 ttttcaattt tacgtaataa atactactac tttcgctgcc atctgaaaag aagcgaacct
    11641 gataataaaa atatagcaga taccacaatc aaatacaata actgatagcg tatatcaaat
    11701 agtgtggcac cttcattcat caccatacgt gcactttcca ctataggtgt aagcggcaaa
    11761 agctgggata ccttctgtac ccagggacgt gccccctcca gtgaaaacca cacctctgat
    11821 agaaacatca tgggccaggt aataatatta attaacccat cagcaaactc ctggctgtca
    11881 cttcgacaag caatcagcaa acccaacgaa atcatacaga acccacccag tgcaaaaact
    11941 acaaacagcg taaataacga acctttgcac tgaaatccat acaataaaac agttccaata
    12001 tacacaacaa ttgttgttgc aagcattaca aacatacgcg acaatatctg agctgttaaa
    12061 aattcaaatg gtcttacagg tgcaacactg aatcgtttta atatgccgtt ttttctatac
    12121 attaccagga cataacctac accatataat gagttaaaca ttgcattcat tcctaaaatg
    12181 ccaggaaaaa gccattcaac atagcggatt cccctaccct taaacgaatg tttagaaaag
    12241 aatgtagtag ttgtacctgc gcctttcaac agttgttctg ctatataccc attgggtgat
    12301 gaatcattga taaaatacgt attatgctca ggatccacca acatgtctat tttaaatttt
    12361 tgtaaactat caacagcttt ttccttagat gaaaactcaa taaaatctat atactttaat
    12421 tttaaaaact cattgaattg tttattactt ataatcgtaa tttcattatt caatggcttc
    12481 accacaccaa ctttgagtac tggcgatgat tcgcttctga atacaacact gaaaccaata
    12541 ataatcaaaa gcgggaaaat aatattccac ccaagagctg acctgtcgcg ataaaattct
    12601 tttgtacgtg caataaatat tgtccatatc ctgtatagca ttgttaagcc cttaattgtg
    12661 ttcctgttaa ttttaaaaac aagtcctcta aattttgtga acgtactacc atgccagtaa
    12721 tatcaacatt gttatcaata agcagcctca ggcaatcatg cggttgttcg gtgtgtattt
    12781 caatggtatg atttaccgta taccactcac aattcatctg gcgtaaaata ttctcatcaa
    12841 ccgttgaagg caatgtaatg gtaactccac ggcaatgttc attaagtaaa ttaatcggcg
    12901 aatccatggc aataatttta ccacgatcca taattgccac aatatcacat agcgtttggg
    12961 cttcctccat atagtgtgtt gtgagtatta tggttttgcc tttctttttt attgattgga
    13021 caatatccca cacatgacgt cgcgcctgag gatcaaggcc tgtggttggt tcatcaagaa
    13081 acaatagttc aggatcattt gctaaagcta tcgccagaag taaccgctgt ttttgaccgc
    13141 ctgatatttt cctgttatcc ctgtccagta tttcttctaa ataacacaga gaaacaagct
    13201 catccagcga agccctgcgt gcatacaaat ttctgaaagt cagtagtatt tctttgacgg
    13261 taagatactg ttgcaattca gtatgctgaa gctgaatgcc aacctcctgt ttaaaatgct
    13321 gatcacgagg cttcccctta tacagtattg ttccgttgtc aggtttttga attccctcaa
    13381 taacttcaat ggtagttgtc ttgccagcac catttggtcc aagcaatcca aaacatatac
    13441 cacggggaat agcaaaactg acatcgtcaa cagccttaac accagggtaa tatttatata
    13501 gatttttaac ttctaatata tagtccattg cttatccata ggtatattgc atttgtatca
    13561 ctaagcagtt attttacttt gtacgcttta catgaataca tcatacatat gattcacatt
    13621 cttcaatgaa ttgaggtttg gggttactat aggcaggatg gtacagtata tgcaagcagt
    13681 tttttgcacg ggtaagagca acatacatca accgccgttc ttcttctata tccgtatatg
    13741 gatcgggcaa caatctatct tttacaccac acagtattac tacatcaaac tccaggccct
    13801 ttgccccatg tatggtcata aacttcacat tatcatctgt atttatcctt gactgcaaaa
    13861 atgtgccctg ccagttattt ctatacagca caccaataga aattttttta tcatactgct
    13921 tagcgatagt atctgcaatc agtgcctcta tttcaaatga gtcaaccttg acaaaataaa
    13981 ttttgccacc gccaccacgc actgacttaa cccagcgccg tgacctgaat ctgtttttag
    14041 caataagtct gtttgaaagc ttcactatct ccttttttga ccgatagtta ctgttaagtg
    14101 aatgtactgt taaattatca aaatattttc gtgcattgac aatatattct atctttgcat
    14161 ctctgaattt ataaattgat tgccagtcat cgccaaccat aaaaatattg gggttttctt
    14221 caggtaaaag caattttaaa agtttaaaat tattttctga tgtgtcctga aattcatcaa
    14281 ccatcacata tttaaaagtt ttatgtattg tattccgtaa cgatatattt ttagacaaac
    14341 aatctataga tttatttatc atatcctcaa aatcaatgac gttatgtacc tgcttatact
    14401 gttcatattc atctttaata gttttgattg cagcataaat atcatgaggc aaatttatac
    14461 tttgaatatt ttttaacgct aatttataca ttactgatga aggaatacca ttaaatgttt
    14521 caacatgctt ttcaactatt tgttttataa ttttctctga cgattcttca tcaagaacag
    14581 ttacctgttt catattttgt ttaataatat actgaaggca aaaagcatgg aatgtggaag
    14641 caacaatatc atttccgcaa ttgcctaacc ctgcaattat ccttttttta agttcatctg
    14701 ctgcttttct gctgaacgta agcaagagca gttcaccagg tttacataat cctttattga
    14761 ttaatgcaat actgcgctgt ataatagtat atgtcttccc acttccagca gaagcaatga
    14821 caagatgtgc accgttttcg gcatttattg cctttatttg atttttgtta gcttttatgg
    14881 acattgtaat aattatttta tcactatgat aaaaacatgt ttctccgttg ctaaaagcgg
    14941 cggaattaat agacattttt tgcctgaaaa aaataagaaa gaaatactga ctatgtatct
    15001 aattaatacg cttgtataaa aaaatcctta aaaaaatatt tctatatttt taaagttata
    15061 acattctttt cgttaatcta atacatcact gccataggtt gaggaagtat aagtattcat
    15121 tttttatgta acaaagtata taatgaacga attcgttcat catatttatt taattccatt
    15181 gccttactaa ttgctgcacg tgcactatct tttattccta aagccaaact tgcctgtgca
    15241 tatttcagcc agccaccata gtaattaggt acaatttttg ttacttcaaa aaagctttcc
    15301 attgctgttt caaagtcgcc catggcaaaa tgtagtttgc catagcgcat gtacccccat
    15361 ggattatcag gcattgcatt cattactttt gcatagacat gcgatgcttc aggatacatc
    15421 tcattatcca gataataatc accttcaaga atatcggtca cccagcaatc accagcaacg
    15481 tttctatgca aggcaaaatc acgcgccata taccacgcct tattacgtaa attgtatgtt
    15541 tgcagcaaca aatcattcat ccactcctgt atcattggtt cattgcgaag gtaaattcct
    15601 gcatcctttt ttgtaaacca gatactatcg tccagaacac cacgttgttt ttcttcttca
    15661 tatagttttg taccaggata caatgcaaca ggcgacacca cacaatcacc aggcaacatg
    15721 gtagcaatca gcttttttgt tttttttata tcactgtatg tttccccttt catcccaacc
    15781 attaaataca ctgaaagata tattccaacc cttcgtgtaa ttgcagcagc atttagaatt
    15841 ttttctacag ttattttttt attatacaaa gaaagtatct tttcagaacc tgattcaaca
    15901 ccatactgta tatgttcaag cccagctttt ttcatggcaa taagcattga ttcatcaacc
    15961 gaatcaacgc gcccctgaca gttccacatc atatacagct ttttttttat tattttgtcc
    16021 gatagttcct gtacacggtc ttttttgatt gtaaaattgt catcccgtat tgaaaaataa
    16081 attattccat atttcttata caatagatta agttcatcaa taacatcatc aatatttctg
    16141 aatcgtacct ttcttcccca gaaagacgga gaatcacaga acgtacaatt gaagctgcaa
    16201 ccacgtgaag ttattatata cttaaattgt tcattgatat ttacacctga aaggttacct
    16261 gcaaactttg caggatatgg caatgtatca atatgtatat tattttgctt tagtatcttt
    16321 tcttttatgg gtttgccttt tttaattgca tctgcaattg aaagcattgc tccttcgcct
    16381 tcaccttcca caacataatc aatatatgga taacgtttaa taatctcctg tgaaaggaac
    16441 gtggcatgcg gcccgccaag tacaacaaca ctatgcggga acaccttttt gattttttgt
    16501 attacggaaa atgtcgtaac gcgattatgt gtatataaag aaaagccaat aatgtgtggt
    16561 tttatagttt ttatctcttt tactatttgt tcagggcttt ttttagaaaa attggctaat
    16621 gttacatcat accctttctg tgacagatat gcagcaatag aaacaagacc aacaggtagc
    16681 aatgtaatat aataatcatg actgtgttct ttttccacac aatagccaag tacaatcttc
    16741 atatagcccc tttattattt tatcgcaggg aatacaatca ttcttgaata ttaacatagc
    16801 ttcttaatta tattttacta ttatgactgt tatgaataat ttgaccaaaa atttactaaa
    16861 ctctgtgtaa tttctatgta aaccccttat ttggtggtat ttttataata aatatgagat
    16921 aaaaatattt tacattaatt tattttacct gcattattga tatcttaatt taatacttag
    16981 ccacatatac cagtaaaaat tataattttt ataattttat ataggggaat atgcaaatta
    17041 ctatctcaaa aaacgaactc tatacaatga ttaaaaaggc ggtgaaagaa gttatttatg
    17101 aagaacgact tgatatatta ttaaaaagta tacctgccat ttcagatgaa gaaatgactg
    17161 aaataaaaaa cctttatggg cataagccac caaaacgtaa agctgctaaa cgaataacaa
    17221 ttgatatatg aaatggacca tagaatattc caaaaatact gttagttttc ttaaaaataa
    17281 tcaatctatt gaagataata ttataaacga aataaaaagg taatccaaaa attacagggt
    17341 caaactgtta atatcaattt aaaaaagctt tctggtgaat gggaaggata ctatcgtatt
    17401 cgcaaaggta aaatacgtat tattatttat cttgattttt taaaaagaac tgtttatgta
    17461 gaaaatgttg attttcgagg aaacatatat aaataatcat gtttttaaaa ctatttaaga
    17521 atctttatct actgatttct ggtttattat cgtttcaatt ttttttgtta atttttcgta
    17581 gtggcttcct tcccagtata ttctgccaca ttgtgtgcat ttataatatg tattacacca
    17641 gctcagtaca cctggcggaa ttcgattttt ttccttttca aattcttcag tattgttaga
    17701 caactcggta acattcccat tacacattat gcatcggctg aatggattta tcttatccca
    17761 taaatccagc ctgtctaata tttctactat ttgcttctgt ggactggtat tgcgaacaat
    17821 taatccccta tcaacaatct ttctcatcag taactgtcgg tcacgagtaa gcaaaatacg
    17881 cttttgctct gctgctatgt gggctaaatc tttatcatcc caggtttcat tatataagac
    17941 atcaaaacca aataaacgaa gatatttaac aagtttacgt aaatggacat cgcagacaaa
    18001 tgcaggtgca cgcagacttt gcggccttaa acgggtaata ttactgattt caaatcgttc
    18061 aaaaacaggg tatacgctga tataatcatc attctgaaca atataatcaa atccaaccga
    18121 aatcccattt acctgtataa gatcaacttc aacatgggga acacccaggg attctattaa
    18181 atcttttata ctgcgtttgc cctcccagct tacagtgaat gcaaccttct ttttatcttt
    18241 tggcaggaag tcattgagtt cttcgtaaaa acgaattgtt acagtatatt ctttcaactg
    18301 ttattctttt ttgaagaagt ttgatatggc tccaggtgta caataacatc cagaactttt
    18361 gggcctttat ctaaaagctc atgctttacg ctgcgggcaa tatcatgtcc atcctgtacc
    18421 gacatattgc catcaaccag tacatgcaga tcaacaaaat atccactacc aacacggcgt
    18481 gaacgaatgc tgtgcacctc tttgacccca tcaaccttca ttgcaattaa atctattgca
    18541 tccacctgcg atttagaagc tcctctgtca gatagttcca tcactacagg ttttaaaata
    18601 tcaaatgcaa ccttaacaat aaacatagca acaacaacag caccaatatg gtcaaagaaa
    18661 actaaagaag gatgtgcaat ggcaagtgct accgcaacta gcgctggtat ggaactcaat
    18721 gcatctgagc ggtgatgcca tgcattggca ataaccgaag aagaatcaat ttcttttcct
    18781 ttttttactg tccatcgata cagccattct ttagatagaa tagaaataaa ggcagctata
    18841 agtgcaattg cttcaggacc tttactgtca ggctcaaatg ccgttattat accattgtac
    18901 gcaatgccaa tgccaacagc aaaaagggct aatgcaataa atgctgtaac aatactttct
    18961 atgcgttcat gaccataagg gtgatcgtca tcagcagggc ttgcccagta tttgatgcct
    19021 acaagtactg caatatctgt cgttaaatca gaaatactat gtatagcatc ggcaaccatt
    19081 gcctgactgt gtcccagtat ccctgcaaca aatttcaatc ctgacagaat gatattgact
    19141 atcattccag tccaggtaac ttttttagct acaatggctt catagtgttt tccattatcc
    19201 ataacgctcc ccaatactta gctttggttt acgaaccaca atagctgcct gcccacttac
    19261 cgggcacctg ttttcacaga taccacatcc aatacatctg tcttcatgaa caactggcaa
    19321 taaaacttca atctgtagtc ctttcttatt tttgacaatc tttttttcta atgttatagc
    19381 cttgttagca accgggcata cttcttcaca tacaatgcaa tttgtttgtt ctgaccatgg
    19441 taagcatata tcatgcagga taacagcagt gccaataaca aacttttgtt tactgttaag
    19501 cgatagcttc tgaattgctc ctgtggggca aacctgtcca cagcgtgtac aggtaaaatc
    19561 acagtaccca atgcgcgata ttaagcgtgg cgtgaacatt ccataaaatc cagcttcagt
    19621 aatgcatgga tgaagcgcat tggtgggaca cacattcata cattgcccac accgaataca
    19681 tgcatctaaa aattgtgatt cgcgcaatgc acctggtgga cgaatgatga agctatcgtg
    19741 tgatgctgca aaaagcgata ctttactggc agcaactatc accgatgacc ccgttgcaat
    19801 gagcaagtct cttcgagtta ttcctttgcc aggatactca atacttttgt ttttcgatgg
    19861 tctggtaaat gtaaaggaaa tagcatcata cttacagttt ttcaggcaat caaaacattt
    19921 aatacactca tgctctattg tgccaacccc gtcggtagct atcgccccca tacgacagcc
    19981 tttaacacat gccccacaat gaatacattt gtctgtatca acaacccgtc ggaataatga
    20041 aacatgtgaa gctatagcat acattgcacc caatgggcat aacaccctgc accagaaccg
    20101 cctgaaaaat gcagataacc ctaacacaac aaagaaaact acagcaaccc atgcccagtt
    20161 gtagtaataa aacggataat cccgtgcctg atatccaggc aatatccatg acagaggctt
    20221 tgaacctatg aatgatacaa acggatataa gaaaagttca taagcacgat aggcaatggt
    20281 aattggatca atatatcctg acactgtaaa accaaagaat gctataagca ttgaacttaa
    20341 caatactaaa aatttgattc tgcttaagtt tgtttgtact tttgtttttc cttttggttg
    20401 tataacagta tcagaagcat caatggttgc acctaacgga cacatccatc cgcaaaaaaa
    20461 ccttccaagc aaaaaagtca aaataaataa cactaaccct aacaatgcat acttgactgt
    20521 atgagttgta atgtatccaa ttgttatact gtgtattgaa agtgaaggat aaaatcctat
    20581 tcctgcatca gaaactggat catgtagata aatgatgctg gcaataaaaa caaaccataa
    20641 taaaagctga ccaattgccc tgtatatggg ccagtgtttt gatttcatac tgttcttgtt
    20701 attgttgaat atgcagtaat attaatactg cccatacctg attgtgccgc caggttaaga
    20761 tagccaatag aagatggttc aataccaaac aattgctttg ctgcatacgc atcaaccata
    20821 acgggatttg taccaaaaat tactgtttta gtttctctta tatccgcagg gctacctgat
    20881 gatgggccat gttttgttaa gatgcgcaat ccatccagta ttattaaatg tacttttact
    20941 gctttgttaa aatcaacaat attcctgtgc aggtttatgt gataatagcc tcggttgccg
    21001 ccaataacac ccattaagtt tttcatggca agggtaagct gtgcagcact atgatgcttt
    21061 gcaatgggca tatttataat acaatcagct tccagtatat cttcatacac atcaagtgta
    21121 tctaaattaa gcccaccagg gactttgact gttttaaatt ttgctctatc catatacaca
    21181 taatctacac caactttttt tgctacttct tcaataccac ttgacctgta gcaaagccgc
    21241 gcattatgaa caggtctatc catgatcgca acgtttgccc ctgctctttt acacattgct
    21301 gcaactgctt caactaagat tggatttgtt gtatgtgcgc gatcaggagt tgaattaaaa
    21361 cccatgttag gttttatgac tactttgctt ccctttttga caaatttttg aattcctccc
    21421 atttcctcaa agccttttac caccatacca tatatagatt tgtctgttaa tgcatttgtt
    21481 tttatcacaa ccagatcggt tttcttttcc agaccaaatg ctttttttaa acctgtgaga
    21541 tagttaccag caagaaatcc tgttgcactg ataattgatg aaaataaatt tttgcgtgtt
    21601 attttcatac tcaccccttt gccgattgtg gcttttcttt ataaaacaac aaaactccga
    21661 tacctgccag gataataagt attcctaaaa caaatgcctg ataactttgc catgaatatc
    21721 caggttcaag taatgaattc acaggatcat cgggatcata atatactgtt acttttttgc
    21781 ctttaggata ttcccgtaat aattcctcac ttgatttatt gacattcttt gctgaaattt
    21841 gaaaaccagt aattttttca ccgtttacta taaattcata cttgatattg gcatggtgtt
    21901 gcctttcacg atcccagtcc ataaatgatt gcgttataac accttcagtc ttaggccagt
    21961 gtaaactttc ctgtgcatta taaatgagtt ttattccagc aagagtgata attataccaa
    22021 agacaaaaag aaaaattaca atttttttct tttctgacat agactgcttt cctcccgtat
    22081 aatagtaata attgttaaat accatgcatg tataactatt taccgttgat ggatacaaag
    22141 cacaaccttg cgcttttatt tttgaataat tacttatttt ataaccgcta aaacgtttta
    22201 acagtaaact tgcatcttcc catactatga tgtaatataa atttttatta aagtatagta
    22261 aaatcataga taaaagtcaa tcatgttata gccattttgc taatgaaccg ttagcagtgg
    22321 tctgtacata gagtattgaa gggaataatt ctttcacatc atcaatgtgg gaaataataa
    22381 aaatctgtcg gtattgctcc tgaagagtgt ataatgcatt aattattgca ttcctgcgtt
    22441 cattatcctg gctgccaaaa atttcatcaa acgccataaa tccaaccgca ccgctgccac
    22501 tgaaatccct gatagcattg ctgatagcta tccgtaaaca aagattggca caatcaattt
    22561 cacctcctga aaatcggctt aacgggtacc acctgccatc atccattatc tggaagttaa
    22621 aagtatcatc catttcaatt gcctggtatc gcccctgagt taattgattg aaaaggttgc
    22681 tggcataatt ggcaataaca ggcattgcca tctgcagcac tgactctctg aatccatcca
    22741 tgattttatc taaccgttgc agcaattcaa attcctgttt agcaacatca atctttttac
    22801 gattttgttt atctttttta agctcatcat caatcttgcc gattgcattc ttgatatcat
    22861 tatacaataa tttttcttca tttaattgct ttattatttt ttctttttcc tgcaatactg
    22921 aatcatagtc attttttact tcatgataca atttttctga aaagcccagg gtagcaatat
    22981 cctcagtaat ttgtttaatt tcaaccgctc tatcattttt ctgtttttca agctgttcaa
    23041 ttctctgctg taaaacagga atctcacgta tctgtgcttc taatccaatg aacatagtat
    23101 gaaacgcctc aagtttggaa tattcttcta ctatatttgc atactcctgt tcatcaaaca
    23161 ttaccgtact caattcctgt aactgcaagc caatgacatc aagattatct cttttctttt
    23221 ttagttcact cagttctttt tcaacatttt ttttcttttc taacaattgc gctttctgtt
    23281 gttgtaaatc atttaaccgt atatctaatt cttttacttc atggtcagcg tttttcaaca
    23341 tgctgtcttt cttttcagca gcactcttca gtagggccaa ttcttttttt tcatattctt
    23401 caatttcact caatagtttt gcaattgttg catcataggc ttcatgcaaa gggcgcaaac
    23461 atacaggaca ttcagcttcc ctgccaagct tttcaatctg ctcaatatgc ttttttcggt
    23521 ctttaacagc cttttcaata ccgcctatat gttgttttat tgtatacagt tcttcctgta
    23581 aagattttac tgtttgttct atgtgttttt ttctgctatc attttgttta attgttgttt
    23641 caaggtcatc aagtttttgc agttcactct gttctgcttt tatacttttt tctttttcat
    23701 gcaactcatg cataagttct tcatattgtt tctgcaacgc ctgctttttc atattcattg
    23761 cacgcgcctg ttcaagttcc tcttttttct ttttacattc aaggtacttt ttctcatgac
    23821 catacagctt ttcatattca gcttttgcat gttcaagctt cttaagtctt tctttggctt
    23881 cagcaatatt ttgcatgcag ttatcaaact gtgcctgtag ttgtgtttgt ttttttagta
    23941 aatcattgtg ttttatgtaa agctcttgct gttctttaaa gtttttctgc acattgataa
    24001 acttttctga aatcaattca tggtttttct gtaatgtttg tacttccttt tctttgtgcg
    24061 atagttcctg atactgtttc tccagtgccg attcttttgc cttaatatca tcatcgctta
    24121 aaagcatcgc ctgttgccct tcaataaatc gctttttatc atttctgtca ttgcgtatca
    24181 tctgtacaat ctcatccagc aagctgaaac ccattatttt tctgatgagt tctctacgat
    24241 cagaacctcc tgtttctgat atctgtctga gttcaccctg tgctgcaaac actgagtgcg
    24301 tgaatgcatc ctgcggcatc cccagtatat cttctattgt cctgttcaca tcagtagcac
    24361 cagttgctat cgccacatca tttttatata atgaagcata ctgattcagg tttttccctc
    24421 taaattcacg tacaatcctg tactgagtat tatcaatttg aaaaactaac tcaattgaaa
    24481 cagaagcttt ttcttccgca aacactgaga ccatatattc ctttgcagtt ggcaaaacac
    24541 cgtaaagagc acacacaata gcattaaaga tggtactctt tccactgcca ttttttccaa
    24601 taatcccaat gagtccttcc ttaaactcaa tggtagcatc tttatattgt ctgaaatttt
    24661 tcaaggtaag cttttgtata atcattagct attcctttat gtcagaaaat tgctcacggt
    24721 attttataaa atagtgttta cccagcgctt caactgcttc tctttctttt tcatcgctga
    24781 ttgattgttt acaatatgct gcaaagactt cttcaattga aatatgttct ttatatgatg
    24841 aactatcgct acgctgctgt ttaaaaattc ttttaggcaa caccacaagc gcctgtggaa
    24901 attgctgtgc aatccacata ttgctcacag caacactttg atgcgcatca atgtcatgca
    24961 gatacacact tacaatagca ccctgcgcaa cctgtgaaga aagttcctga atatttttct
    25021 taatatcttc tacagaatac tcgctgcacg tttttatatc aaatctcacc caatcacgga
    25081 ttgtaatagg attaaattct atgtttgttt ttgtgccgat agtaactgtt acaaagcctt
    25141 tctgcttgcc agcttcccta tcgctgaagc gttccgttga tccactgtag cagacattgt
    25201 cccaccgcgg taaaatgtta aaattatgcc agtgccccaa cgctacgtaa tcaaattctt
    25261 ttaaaagtga aaagtattct tcaggaaata tccgctcacc atattcatca agcagatagt
    25321 ctttcccaat cgacgtatgc attaaaagta tgttatgccc ctgccttggc tcaatctgct
    25381 ttatttgcgt tataaattct gcatcttcat ggatatgcgg caaacagtgc agatgaatac
    25441 catcaagcga aaagcactca tactcaccct tatacacagc atatacacca tctatcgtct
    25501 gcaatgcctg caaaatggga ctggtgtata tggtctttgg tgtacagtga tttccagcaa
    25561 taacaataaa tggaatacct gcctgcgtta ttctttttat gtgctgtaac ccctgtatga
    25621 gcgcacggtt ggaaggcgaa gaacggtgga ataaatcccc agtatggata acaaaatcag
    25681 gtttatactg aataatactg tcaacagcat ttgcaaatgc gttatataca tcagcttcac
    25741 gctgattgat gccatcatca ttaactttat caaaatcatt aaaccctaaa tgggt
//
LOCUS       DAORYL010000040        25538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1630_length_25538_cov_13.191304, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000040 DAORYL010000000
VERSION     DAORYL010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 25538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25538
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1630_length_25538_cov_13.191304"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1771)
                     /gene="flhA"
                     /locus_tag="PLV81_08615"
     CDS             complement(<1..1771)
                     /gene="flhA"
                     /locus_tag="PLV81_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOM10196.1"
                     /translation="MAEQTYDFSWMQKSDILLGIGVIAVVTMLVIPLPTFLLDFLMAI
                     SIMLGILILLVVMYVPRSFDFSVFPALLLITTVYRLALNVSSTRLILLQGAAFDGKII
                     RTFGEFVVGGNYVIGFIVFVILVAVQFVVITKGATRTAEVAARFTLDAMPAKLMSIDS
                     DLSNGIINEEEALRRRREVRKEADFYGAMDGASKFVQGDVKVGIIITIVNIIGGFIIG
                     MVMRGESFDVAVHTYTLLSIGDGLVAQIPSLLITTATGIIVTRAVSEDSLGKDLSAQL
                     GSQPRALMLTAAALGLSAIIPGFPKITLILLALGIGALGYLLKQTEEEQIEKTKIEQK
                     EAALKTHKPESVIPLIQVDPLEVEIGYSLIPLADPDQGGTLLDRITNIRRRSALEMGL
                     IVPPIRIRDNMELAPQDYSILIKGVEVGHGSLQVGKLMAMDPGTVEEKIEGVEFTEPV
                     FNLKAIWIDPDNRDIAERRGYTVVDCPTIIATHLTEIIRRHADEILGRQEVQQLIDTI
                     KNDYPAVVNELVGDKKLSIGEIQKVLQNLLRERVSIRNMVTILETLASYSDYTKDIGL
                     LTEYVRVALARQICREYADKNNTL"
     gene            complement(1787..2944)
                     /gene="flhB"
                     /locus_tag="PLV81_08620"
     CDS             complement(1787..2944)
                     /gene="flhB"
                     /locus_tag="PLV81_08620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HOM10197.1"
                     /translation="MKWYEDINLQILSLHTSSAIVFDLQLFAAEDEGRTEEPTEKKLR
                     EAREKGQVAKTEELPQALVVIFAMITIVVFCGFIFETIIRMMHYYLTSFSHFQLTERS
                     LFHEAIATGIESFKILLPIFVATVIAALLGNIAQVGFQISTHPLKFDLSKIKFDPATM
                     ARKIFFSRQVAMNLFKSIFKVVVIGFVCYLVISNDFDELIKTPDISVGLAFQNISIIA
                     LKIIIWSTVCLLVLSIPDYIFQKREYIESLKMTKQELKEEWKETVGDPHVRARLQEMQ
                     RELIMRNMIREVPKADVVVTNPTHFAIAMRYEPEAMYAPMVIAKGVDAMALRIRQIAL
                     DNNILVIENRPLAQELYQRVEVGDIIPEDLFYAVSLIYAEVYKKRNYKAAI"
     gene            complement(2941..3720)
                     /gene="fliR"
                     /locus_tag="PLV81_08625"
     CDS             complement(2941..3720)
                     /gene="fliR"
                     /locus_tag="PLV81_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOM10198.1"
                     /translation="MEYFVYQFQAFFLVLVRMTAMMVIAPFFSSGAIPLLMRGALSFF
                     VSLVIFPVLSISNAALTGNMGVYYLMVIQEIIIGIYIGFLVSVVFSAFQLSGQYFAVQ
                     IGFGINEVIDPLAQISIPLVGQLKNIIGLLVFLAINGHHFMIKAIFHSYELAPAFALK
                     GESIQAFLKFLAHSFSGMFVIALKISLPVIAAVFLVSVSLGILAKAAPQMNIMMLGFP
                     FKIAVAFGMMILISPLIVRVMNVSLERTFQFIVKVIRYWPV"
     gene            complement(3720..3992)
                     /gene="fliQ"
                     /locus_tag="PLV81_08630"
     CDS             complement(3720..3992)
                     /gene="fliQ"
                     /locus_tag="PLV81_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HOM10199.1"
                     /translation="MTDVEVIKILREALMVTMVVSAPILGIGMFVGLIISIFQTTTSI
                     QEQTLTFVPKIIAIFAALILFAAWILHTLVAYTKGLFMMISKLGAG"
     gene            complement(4002..4724)
                     /gene="fliP"
                     /locus_tag="PLV81_08635"
     CDS             complement(4002..4724)
                     /gene="fliP"
                     /locus_tag="PLV81_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776506.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HOM10200.1"
                     /translation="MCMDVAFAQKAGNVRMPIPKIGFDIEEAKTPKDVSLSLQILFLL
                     TILTLAPSIIMMMTSFTRVVIVLDFVRRALSLQQMPPTQVIVGLAIFLTIFIMSPTLT
                     EINDKALQPYLKGQISNEEFYNRGMEPLRKFMLKQTRKKDIALFIELAKLERPKTEDD
                     VPTYCLIPAFMISELKRAFEMGVYIFIPFIVIDMIVASALMAMGMIMLPPIMISLPFK
                     IVLFVLVDGWNLLTYELVKSFM"
     gene            complement(4803..5564)
                     /locus_tag="PLV81_08640"
     CDS             complement(4803..5564)
                     /locus_tag="PLV81_08640"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOM10201.1"
                     /translation="MKKFFYHSFLLCLIFILCPITMNYRPNDTMLYAKETRTVKAVKN
                     DKEKTRAEKSNEVVDVKENATTIAETADTEKKDNDAQKGDYLYNYEQPQVEEESYVWL
                     IFKTIFVMGVLIAGFYYFFKFITQKVGITAIGQEVITILSVVPLGQNKFLQVVDIAGR
                     VYVLGVSDNNINLITEIKDKDDIDRIRLLSSKSTPLKQGGFQEYLSTQLGKVLDKMKN
                     PGRPSQRFASEEKTIHDDVLISYRQRLKKLNGDDA"
     gene            complement(5639..6694)
                     /gene="fliN"
                     /locus_tag="PLV81_08645"
     CDS             complement(5639..6694)
                     /gene="fliN"
                     /locus_tag="PLV81_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HOM10202.1"
                     /translation="MGDGSLSQDEIDALLQGADDMLSGTARVPAGEFAPAQAALSPVE
                     RESVADFLNSSMGSVMPALSGYLGKNIIIGNALVEVKSADQVRAEFPKQYVQIVVNFS
                     GGLNGKNVILLSMNDAGVISSLMMGDDSGTPPATLTEAHQSTLQEFVSQMMSTIATQL
                     SNKIGKGVNTTAPDLKVVQGNIEVPGTPDVVKVTYNLTIQNLINSKMYHLMNIELAGE
                     IARGAYVPQMQQPEYRPEPAQPRTTSQVGISPVRFPPLGEGMQSIPTGNINLLLDVPM
                     TLTVELGRTRQLVKDILGLGEGSIIELDKLAGEPVDLLVNGKLIARGEVVVIDENFGV
                     RVTDIVSPAERIAKMQS"
     gene            complement(6776..7312)
                     /locus_tag="PLV81_08650"
     CDS             complement(6776..7312)
                     /locus_tag="PLV81_08650"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HOM10203.1"
                     /translation="MGDEERVNVEEIEEQEKPEEARATGAFETSRIIKILLYIAGAIL
                     AIFIIIGISYLVAKYVQEQRYQREQDIVLAPPPPPLAHFDMPSFSITTNDPEPHFAKI
                     TISLGYEENVELNAELVQRTPQMQHVVNILLRDKSYEDLNSVEDTVNLAEEIKAHINV
                     LLLKGKVKEVYFREFVVN"
     gene            complement(7512..8291)
                     /locus_tag="PLV81_08655"
     CDS             complement(7512..8291)
                     /locus_tag="PLV81_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOM10204.1"
                     /translation="MPKRKKMELPPPPPWLTSWGDLNTLLLTFFVMMFDISPIIGQDF
                     NLVLSSFKGSLGMMQGGYSLARGRMEEIGLNMLNLPSSEQGRALAKMLKRAVEAFKPE
                     IQAKKVRVREDERGLVITLSSDAYFEPGSAKLKDDIIPILKKVALIIKSMPNYVRIEG
                     HTDNRPVPPRGLEEGYATNWELSSARAVNVLRYLIEETGVNPKQMSAVAFGEYRPIDD
                     NNTPEGRAYNRRVEIVILRDKGVIEPTTPGIVRPLPDEEWR"
     gene            complement(8291..9070)
                     /locus_tag="PLV81_08660"
     CDS             complement(8291..9070)
                     /locus_tag="PLV81_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HOM10205.1"
                     /translation="MDLATVIGLIVGFTNIIMGIIFAKGNPLLFFDIPSVFITIGGSF
                     CALLISNPLSKVLGIWKVAKHAFFPPKYNPNELIITLVSFSEKARREGLLALEDDLDE
                     LDDQFLRKGIQLVVDGTDPELVRRIMETELENIMARHDEGKKLFDDWATYAPAFGMIG
                     TLLGLVLMLVNIEDKSAIGPGMSAALITTFYGAIMAYLVLYPISSKLGFLNNQEVLMK
                     LIMIEGTLSIQSGDNPRIVKDKLVSFLDPRLREAITSEVGD"
     gene            complement(9226..9417)
                     /locus_tag="PLV81_08665"
     CDS             complement(9226..9417)
                     /locus_tag="PLV81_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004447972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HOM10206.1"
                     /translation="MITLHKMNGEEFILNANHIETIESKPDTTITLTNERKYLVKESK
                     DEVLSRIYEYYHKVLTGFK"
     gene            complement(9414..10325)
                     /locus_tag="PLV81_08670"
     CDS             complement(9414..10325)
                     /locus_tag="PLV81_08670"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOM10207.1"
                     /translation="MNTESLYQALNQGLLQERYALIKRNITKNKVLIEKWGGLQKVVA
                     LLSGKHVVVCGAGRSLNDALKVLRKYQYRKDLAIIATDMAYRPLVLAGIKPHFSISCE
                     AMPVSYYAFLPTSSCHLLAFSGVSSHTLATWKGKISFYNWMIYESPFDALWKLAGQHL
                     GYVATASIVTTQAISLALGCSIASLTMVGNDLAFKDNYYAQHTVVCQRFIMHYNRFST
                     ANSNDYAMIHRKKMYMVKRRESIYYTDHQFLAARQWLENLFTNVTIPVFDCSIPGCSE
                     NKVTKVTISKVMNTLYPDKPIKKRGRT"
     gene            complement(10359..13235)
                     /locus_tag="PLV81_08675"
     CDS             complement(10359..13235)
                     /locus_tag="PLV81_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10208.1"
                     /translation="MSKRLLWIYSILLTFFFAYTVHAASMFHPRYTWYSIKTSHFWIH
                     YHDGLEHEALRLATIAEEVHEKLKENINWQPFFRTDVVLCDTTDMANGFSMPFPYNRV
                     QLFVVMPHVEDAGLSNTDDWLRMVFTHEYTHTLTMDMIGGIPEATRYTLGRVCFPNIL
                     MPVWAVEGYPVLQESQRKPWGRLNANYTLMVLRQEVYSKNLKDISLASNFPREWPAGM
                     VPYLYGGLFVEFCTRNYPEKDFGEIFKENADNILPFLMEKNFNDVYGARAIALWQIWQ
                     KELINDLNDQIAEIKKRGISDYTVLTSTGFYTSFPRFYNNDIVFIAMTNKEKPSLVKY
                     NTHNKTFSRLAYVNMPNSLAVSNKTIIVSDVEYYQSFDLFRDVFIYDTKYSQKTKGLR
                     VQYIEPFKSGYVAILYDKGKYSLVQLDATFTIQKYFIHNTPFQISYLRVLGDSSKAVF
                     SIVHNGFSDIALYDFTTEKMLLLTNDSFVDIHPTFHPDGQKVIFSSDRDGVFNLYEIN
                     LISKKISRISNVIGGAFFPDISNNGTTIVFSSYEANGYNVALMPYATGQSQELSPIPF
                     TIQQYEEHKELLQQPYNPIYSVIGPWWAPYVYSEELYPDKYDNHIGFNTSGNDTLYRH
                     SYSLGLDYALLQKRMTLALDYIYSGLYPDLFISYKDEGIIFDDTFPWEPPDTVAYMRR
                     TLKRKVSSGISIPFQKFYNYNQLLLYYTYEKTMVSEYYSNTVFTHDIVLAGSHVVFFH
                     DGTYTGSFSVSPEDGMVFESYADIYDSSLSSDISYSKVRGGLYYFFRGFDNDVLAFML
                     KGGYAHNAPYYLYPYNIGRYAKGKSQSIPSDEDAYSMRGFAKDMFYGNRLAVGSIEYR
                     IPLFRKDVGYRMLPLMFRDMWLTPFVEYGNIWIDSTSLNDFKYSIGSELHIRITAGYW
                     ADIEGFAGIVKGYGDYGELQVYFGIATGIEGALKQHTIIRQVIQ"
     gene            13687..14256
                     /locus_tag="PLV81_08680"
     CDS             13687..14256
                     /locus_tag="PLV81_08680"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOM10209.1"
                     /translation="MHKWHIEKRDTVNKQRIFSIEKLLCYHPAKKVNHDFYILNNPDW
                     VNIVTVDTDGNIVLVKQHRLGTDEYTIETVAGLIDPGEDPYQSAHRELLEETGYRAQE
                     IVLLKKLAANPAIMNNYIYFYCALNAQKVQEQSLDLAEDIEIVTLSPQQVLEFIQKGT
                     INHSIIITALLLYFLSPYSTIPKDKILLY"
     gene            14266..14682
                     /locus_tag="PLV81_08685"
     CDS             14266..14682
                     /locus_tag="PLV81_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10210.1"
                     /translation="MITGKLIIVSLFLLVQSTAPDFSITLSYHPAPFYYEEWHITIAR
                     IGQHAYIETTSPQYARKKRSIPIDEYSSLVEKIVSSGIWNCKDCYSYGNYYMLTITKG
                     NYRHKCNIEYAPQKNSCNNATQIIRFIINTANNYLQ"
     gene            14817..15854
                     /locus_tag="PLV81_08690"
     CDS             14817..15854
                     /locus_tag="PLV81_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10211.1"
                     /translation="MKIKYVIWLLPIVLVFSSLTFSQQTTQENVVQPDVQSPASPEVK
                     KEEIATAALKAVSFNNGVNTFVNSKVKFELFSKDNVATDKILYKLDAGNELEYKEPFA
                     IYEEGKHVISYYAIDKVGNKEDPKAFKVVVDNTPPVVNVSVSAPVIKSGDKLYASKNF
                     TFVVEAKDALSGVQSLEYSIDKTNYQPYLTPFTIPVEGNISLSIKAMDNVTNYTEKYT
                     MVAKDENGNPINLTDGNVSIFVDNKAPVVEIKPDKELLDKNGRKVATADYSYTISASD
                     AESGLSSILFRLDGKGEFKAYTGAIKFDTNGEHMIEAKAVDKVGNVSPVAVLSVFVDI
                     IAPDTTIKAIE"
     gene            15955..16287
                     /locus_tag="PLV81_08695"
     CDS             15955..16287
                     /locus_tag="PLV81_08695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOM10212.1"
                     /translation="MFIETTKLDSILVITPHEDIIRENSEDLERAIFTNINKSTTDVI
                     LDCKHVQYINSFSLGTLVKIHQKLEENSRKLHFLNVAPSIKTLFKITRVLDYFSLIDS
                     MDNVKNKK"
     gene            complement(16330..16818)
                     /locus_tag="PLV81_08700"
     CDS             complement(16330..16818)
                     /locus_tag="PLV81_08700"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM10213.1"
                     /translation="MLSKAILIALLCFVPLQSQFASSVCTEATALAKAKEYSKAKELF
                     IKAIGLNPYYYVAHYGYGKACLYSGDINNAIKHLDIAVTLDATSARGWFYLGFAYFFA
                     KNYVKANFCFKEAYRLDTGFVESLYNIAVIYEITGDTYKSKVYYDKYFFEKNKKDTGL
                     LF"
     gene            complement(16808..17251)
                     /locus_tag="PLV81_08705"
     CDS             complement(16808..17251)
                     /locus_tag="PLV81_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10214.1"
                     /translation="MNTTMLSTSLMIFTTIWGLCVLLLWMRPRIELIWKIAASGIYGF
                     YVWFFYKDLVKGYKQFEKDWYLVTIAFIKEFSALIFANMFFLWPLALIIIFYKADDIG
                     AERLLKFMCIFTLVVWLVFTLYTFYSKQIDTFLFHTLREMIPNAQ"
     gene            complement(17258..18247)
                     /gene="pdxA"
                     /locus_tag="PLV81_08710"
     CDS             complement(17258..18247)
                     /gene="pdxA"
                     /locus_tag="PLV81_08710"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOM10215.1"
                     /translation="MQIRKPRVAITIGDPAGIGPEVTLKALCDFETLPCLPLVICRVG
                     VLQQYYHHLMPSWDILNFNEIQRDTVIQKPYFINIHSDVPIPQPSCGTELTGKESLAY
                     IDAAITLWKLKMIDAIVTGPVNKSLIEKWVPFMGHTEYIAEQIHEDNPRMLMYSGKYN
                     VMLVTTHVPVFKLKDVIDYAVLMETIQICESSLTRIMKKKPKIAICGLDPHCGDNGAI
                     GNFDKDVTAKVVKDAQAQGIIIEGPFSADTIFIPQRWEQYDCIIAHYHDQGLIPFKML
                     AFDEGVNVTVGLSIIRTSVDHGTAFDIAGKDCADYSSMKKAIELASFLALKEE"
     gene            complement(18237..18659)
                     /locus_tag="PLV81_08715"
     CDS             complement(18237..18659)
                     /locus_tag="PLV81_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOM10216.1"
                     /translation="MALAKSGGDVANNVIGENSYFSGKFLINGSLRIDGRFEGKYLQA
                     DQLYIGPNGKVKTNINAVSVIIEGLVIGNINATNRVLLMPTAKILGDIKTPELIIQNG
                     VILEGRCTIANDLKISAKDLIENEYEKNKLIPEEFLAD"
     gene            complement(18800..19573)
                     /locus_tag="PLV81_08720"
     CDS             complement(18800..19573)
                     /locus_tag="PLV81_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10217.1"
                     /translation="MDEREEKAKQIVRSIRALEGVLKTSPDAKQRERVKKDLKSLRDK
                     LQELYPDVELDELIEAILTDDLIVTPTGKSELETLEYLKNVEIEKISNFKDDNEINIA
                     ASILKHFSEKIWGVIADQYTKLDYSNSLERDSLYRKLDECQRALKIFQQTVDDIEKAN
                     TSAHISQLNLMRMRQGRLFLMDLFSFFKDVNDFVTKILADTEFGGTMVLNADDKITYA
                     RYDMYKTFEGLTVKEALRYLKGFVQEVLQIIKVPDKTKF"
     gene            19755..20678
                     /locus_tag="PLV81_08725"
     CDS             19755..20678
                     /locus_tag="PLV81_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HOM10218.1"
                     /translation="MEFVKKYYLLHLFILLCLIVAPLESAPAAIIPVINDLSRSNDTI
                     TLLRKDIRKTLYTIKSNRSVEELPELVMYKYIVKKNETFWDIVSITNLDLDTIVSVNS
                     LSAPSDIKKGLILYIPNMRGIIHEKSQNESYDTIAMSYRIPVQYILKVNRIVTNEHKQ
                     YIFIPCGTLSDLDRSLFLGTAFVSPLTVGKLSSYFGVRKDPIDHAHKFHAGVDIACPI
                     NSPVYASRDGVVVFSGLLGGYGNLVIIKHSRDYFTLYGHLNKILVQKGAIVTAGQLIA
                     KSGNTGRTTGPHLHFEIRKNSKPINPLLLLR"
     gene            complement(20680..22674)
                     /locus_tag="PLV81_08730"
     CDS             complement(20680..22674)
                     /locus_tag="PLV81_08730"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOM10219.1"
                     /translation="MGLLEKALQYKQEINKKGKETLIDTIIGPAETEFINDRKDDAST
                     ALQDDGNTIELSNGDLKSIEPIEDELFILPDDNTPAKDKVETDTTNYDTIIMQDQELI
                     LNSEPDYLGPEDEPDLKLLQNNSIQETNEFKDKDLDNRSDGNPKFRIIESMEDNKDSM
                     RVMQEGDTTKQTISTEYSIKPDKKFQDFVVLYEIGKEIIKAHSLNELYDAILFSVMGQ
                     IGSASATLLVVSADDENSLIVADSRGITIKNKNIRFDITKGLFNILMQSKGVVDIEQF
                     KQETQYRDEYYQLIAIDARLFIPLRYDSKVFGALVLGEKITIGDYSEEEIDFIERINE
                     FAAITYEKVHKMEQLENDIRKSVDDRKFWNELDEYKQSLRKYPQKDHFKVSAHSIMKA
                     NGFLSYAVYIKNNKDGRYSLLLTDEEDMLLLKDSANSINSDSAFIDYILSLHSAADLE
                     DFDRNRAIREVFSESSIRKMTLFWINPFKVANEMLGFFCVFRLRDYQRREIQFERFKV
                     MTDVLLSHYLTLQLIESQYVNYSDVILPVFNRIDKELANVTRLTIPLTIILFTIKNLK
                     RYYTIQGLEKTQELMNKIEAVIISRLSDGDFTARIDRNKFLIVLPGKNKKFAVPFANA
                     IKNEMVQQFSDKELQLLVTFLMAEYPEDGTDVYSLLDAID"
     gene            complement(22680..24983)
                     /locus_tag="PLV81_08735"
     CDS             complement(22680..24983)
                     /locus_tag="PLV81_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10220.1"
                     /translation="MEHKVRELEEITDIPEPVIDLTEEDVIIDMPAEEKVSGELEEIT
                     GLQDITTSTLIIEDENPPLQIQKRGDDDIISITATVDAINTIKVVKHTAVHGKLQKED
                     RLFEDIPEPLHSSKSPKEESHIKDSYIDTTIDEENRQIKGKIYYDKNIDALVIEDFSE
                     DEELAQKEQEELKEATSGYEFEEENAHDKPLRPDEDNVIGDESKINKPEADVITIEIP
                     PEVRNKIEEKDIEDFQFINLDEAEQIASEDILILSEDDLIEELDEYDLIPIETNGEEK
                     KSKEKKKEKVTSDVKVSPKKHEPEKKEVVDVKPQYVELSQEAIIKPDKEKKKPEPVKP
                     IQEEIIQKEESIDLKQEEIPVTEVIITEAPQIDTEVKEKPQDKPEPAIHEKNIDIITL
                     EEEKEFVPASVTTKTTDDTFDTKNLIRDYAQFNIETIPDDLAGAANQKIMIIDDAGVE
                     VKAHLSMEENPIDDIDKLVSGMIEITEGEAKILKEASSKEEDEYIAPILTGTLPTFQD
                     RMIEFEEEYKFKDDDIVFIDNAVLVDEYDKYLKVIDDYYETKTVKKSSSTVELFGLTK
                     DEFEFFYDILFEKEYETIKASKAFKDVTVGRDHPEKDATLVKQFKYIQAKPQSLSDKE
                     KKSIEENIESPVAVVIEENIDDIVERLKKIKPQVDIKKLLAQMPHQPAKLYDVDESKS
                     DTVVEEKPAKQEEITNITDQVIILDSKEDVNRFVDTLPVEKQENIRRLLKYLDSLFEK
                     LPEDVIKNFANSEYFDLYVKVLNELGV"
     gene            25300..>25538
                     /locus_tag="PLV81_08740"
     CDS             25300..>25538
                     /locus_tag="PLV81_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10221.1"
                     /translation="MKLTHKLLCVAIAIVLLPTTSFAIVDLGMYGGYTFAGKFDAPTI
                     SNADTNGWQYGFIGHLNGSVIPMILSMGIGGFYQKS"
BASE COUNT         8008 a         5207 c         4039 g         8284 t
ORIGIN      
        1 aaagcgtatt gtttttatca gcatattccc tgcatatctg acgtgccagt gcaacacgta
       61 catactcagt cagtaaacct atatctttag tataatctga atagcttgcc agtgtttcca
      121 gaatggtaac catatttcta attgaaacac gttctcgcag gaggttttgc aatactttct
      181 gtatttcacc tatgcttagc tttttatccc caacaagttc attaaccaca gcaggatagt
      241 cattcttaat tgtatcaata agctgctgca cttcctgcct gcccagtatt tcatccgcat
      301 gcctgcgtat aatctcagtg agatgtgttg caataatagt gggacaatcc acaacagtat
      361 atcctctgcg ctcagcaata tctctgttat caggatctat ccatattgct ttgaggttga
      421 ataccggttc ggtgaattca acaccttcaa ttttttcctc aacagtaccg ggatccattg
      481 ccatgagttt tcctacctgg aggcttccat gcccaacttc aacacctttt attaaaatgg
      541 agtaatcctg aggtgcaagt tccatattat cccggatacg gattggtggc actataagtc
      601 ccatttcaag ggcgctacgt ctgcgtatat tggtaatccg atcaagtaat gtgccaccct
      661 ggtcaggatc agccaggggt ataaggctgt aaccaatttc tacttcaaga ggatcaacct
      721 gaatgagtgg tattaccgat tcaggtttat gagttttcaa cgcagcttct ttttgctcta
      781 tcttggtttt ttcaatttgt tcctcttcag tttgttttaa cagataccct aatgccccaa
      841 tacccaaagc tagtagtata agagttattt tggggaaacc cggaatgatt gcagataatc
      901 ccaatgctgc agcagtaagc atcaatgcac gtggttgaga acccaattgc gccgaaaggt
      961 ctttccccag gctatcttct gatacagcac gggtaacaat gataccagtt gctgttgtaa
     1021 taagcaatga tggaatttga gcaaccaggc catcgcctat cgacaataat gtgtatgtat
     1081 gaactgctac atcaaaactt tcaccccgca ttaccatccc tatgataaaa ccgccaataa
     1141 tattgactat tgtaataata atgccaacct taacatcgcc ctgtacaaat tttgaagcac
     1201 catccatggc tccgtaaaaa tcagcttctt tacgaacttc acgcctgcgc cgtaatgctt
     1261 cctcttcgtt gatgatacca ttggaaaggt ctgaatctat actcatgagt ttagccggca
     1321 tggcatccaa tgtaaaacgg gctgctactt cggctgttcg tgttgcacct ttggtgatga
     1381 caacaaactg cactgcaaca aggattacaa atacaatgaa tccaataaca taattgcccc
     1441 caacaacaaa ctcgccaaat gtgcgtatta ttttaccatc aaatgcggca ccctgcaaaa
     1501 ggattaagcg ggttgaagaa acattaagcg ccagacgata aacagtggtt ataagcagca
     1561 aggcagggaa tacagaaaaa tcaaaagagc gtggtacata catgactacc agcaatatca
     1621 ggattcccag cataatgctg attgccataa gaaaatccag aaggaacgtt ggcaacggta
     1681 taaccagcat ggttacaaca gcaataacac caatacccag tagaatatcg ctcttttgca
     1741 tccatgaaaa atcgtatgtt tgttctgcca tacgctacct caacgattat attgctgctt
     1801 tatagtttct tttcttatac acttcagcat aaattaacga aacagcataa aataaatcct
     1861 caggaataat atccccaact tccacacgtt gatataattc ctgtgccagt ggcctgtttt
     1921 caataaccag tatgttgtta tcaagagcta tctgccgtat ccttaaagcc attgcatcaa
     1981 cacccttagc aatgaccatt ggcgcataca ttgcttctgg ttcatatcgc atagctatgg
     2041 caaaatgtgt tgggtttgtt actaccacat ctgctttggg tacttcacgt atcatgttgc
     2101 gcataataag ctctcgctgc atttcctgta accgtgcccg tacatgcggg tcaccaacag
     2161 tttccttcca ttcttctttc aattcctgct tggtcatttt taatgattca atatattcac
     2221 gtttctggaa aatataatcc ggtatggaaa ggacaagcaa acatactgtt gaccatataa
     2281 ttatcttcag ggcaataata gagatatttt gaaatgctaa accaacactt atgtcaggcg
     2341 ttttaatcaa ttcatcaaaa tcgttgctga taactaaata acatacaaag ccaataacaa
     2401 ccactttaaa aatagattta aatagattca tggcaacctg gcgggaaaag aatatcttgc
     2461 gcgccattgt tgctgggtca aattttattt ttgaaaggtc aaacttcagg ggatgtgttg
     2521 atatctgaaa tcccacctgc gctatattac ccagcagtgc tgcaatcaca gttgctacaa
     2581 atattggcaa tagtatcttg aaggattcaa tgccggttgc tatcgcctca tgaaataaac
     2641 ttcgttcagt tagctgaaaa tgtgaaaagc tggtaagata ataatgcatc attctgataa
     2701 ttgtttcaaa gataaaacca caaaacacaa caatggtaat catggcaaaa ataaccacca
     2761 gagcctgagg caattcttcg gttttggcta cctgcccctt ttcgcgtgct tcacgaagct
     2821 tcttctcggt aggctcttca gtcctgcctt catcctcggc tgcaaaaagc tgaaggtcaa
     2881 aaactatcgc actactcgta tgtagcgata gtatctgcag atttatgtct tcataccatt
     2941 tcatactggc cagtacctga tcaccttaac tatgaactgg aatgtccttt ctaacgacac
     3001 attcataact ctgactatca gcggcgaaat aagaatcatc ataccaaatg ctacagcaat
     3061 cttgaacggg aagcccagca tcataatatt catctgtggt gctgcctttg ctaaaatccc
     3121 cagactaact gaaacaagaa ataccgctgc tatgacaggc agcgaaattt taagagctat
     3181 cacaaacatg ccgctgaatg aatgagctaa aaattttaaa aaagcctgga tgctttcacc
     3241 tttaagcgca aatgccggtg ctaattcata cgagtggaaa attgccttaa tcatgaaatg
     3301 gtgtccgtta attgctaaga atactagcaa accaataata ttttttagct gccccacaag
     3361 cggaattgaa atctgagcaa gggggtctat gacttcattt ataccaaagc ctatctgcac
     3421 ggcaaagtat tgcccggaaa gctgaaatgc tgaaaataca acactaacaa gaaacccaat
     3481 atatattcct attattatct cctgtataac cataaggtag tacacaccca tattgcccgt
     3541 caacgcagca ttgctgattg aaagtacagg gaagataacc agtgacacaa aaaacgaaag
     3601 cgcccccctc ataagtaaag gaatagcccc acttgaaaaa aatggagcaa tcaccatcat
     3661 tgctgtcata cgcaccagca ccagaaagaa tgcctgaaac tgatatacaa aatattccat
     3721 tatccggcac ccaatttaga aatcatcatg aataatcctt ttgtatacgc aaccagtgta
     3781 tgaagaatcc acgctgcaaa cagtatcaaa gcagcaaaga tagcaataat ctttggcaca
     3841 aatgtcagtg tctgttcttg aattgacgtt gtggtctgga atatagagat gataagccct
     3901 acaaacatgc caattcccag tatgggggca gaaaccacca tggttaccat taatgcttct
     3961 cgcaaaattt ttattacttc aacatctgtc atagttttct cctacataaa tgacttcacc
     4021 agttcatagg taagcagatt ccatccatcc accagtacaa aaagcacaat cttaaacggt
     4081 aatgaaatca tgattggcgg aagcataatc attcccattg ccataagggc acttgccaca
     4141 atcatatcga taacaataaa cggtatgaag atatatacac ccatttcaaa ggcacgtttt
     4201 aattcgctaa tcataaacgc aggtataagg caatacgttg ggacatcgtc ttccgttttt
     4261 ggccgttcta attttgcaag ctcaataaac agtgcaatgt cttttttacg ggtttgtttc
     4321 agcataaatt tgcgcaatgg ctccataccc ctattataaa actcttcgtt gctgatctgc
     4381 cctttcagat acggctgtaa cgctttatca ttgatctcag taagggtagg cgacataata
     4441 aatattgtca gaaaaattgc aagtcccaca ataacctgtg taggaggcat ctgctgcaat
     4501 gacagggcac gccgaacaaa atccagtaca atgaccacgc gagtaaatga ggtcatcatc
     4561 ataattattg atggtgccag tgtcaaaatg gtcagtaaaa ataaaatttg aagacttaaa
     4621 gacacatcct tgggtgtttt tgcctcttca atatcaaacc ctatctttgg tattggcatt
     4681 ctgacattgc cagctttctg ggcaaaggca acatccatac acatagcacc aatgccaaac
     4741 aacattacca cggcaataaa tataatgatt atatgtttat atttaatcct tttcataacg
     4801 ccttacgcat catcaccatt taatttcttt aaccgttgtc tgtatgaaat cagcacatca
     4861 tcatgaattg ttttttcttc actggcaaac cgctgtgacg gccttccagg atttttcatt
     4921 ttatcaagca ccttacccag ctgtgtggac agatattcct ggaaacctcc ctgcttaagt
     4981 ggtgtcgatt tagaactcaa taaccgtatt ctatcgatat catccttatc ttttatctca
     5041 gtaatgagat taatattgtt atccgataca cccagcacat atacccttcc tgcaatatcc
     5101 actacctgta aaaatttatt ctgacccagt ggcaccaccg acaatatcgt gattacctcc
     5161 tggccaatag ctgttattcc cactttttgc gtaatgaact tgaaaaaata ataaaaccct
     5221 gcaataagca ctcccatcac aaagatagtt ttaaaaataa gccatacata tgattcctct
     5281 tcaacctgag gctgctcata gttatacagg taatcgcctt tttgtgcatc attgtctttt
     5341 ttctcagtat ctgcagtttc agctatagtg gtagcatttt cctttacatc gacaacctca
     5401 ttgctttttt ctgccctggt cttttcttta tcattcttaa ctgctttaac agttctggtt
     5461 tcttttgcat acagcatagt atcattaggg cgatagttca tagtaatggg acataatata
     5521 aaaatgagac ataataaaaa actatgataa aaaaattttt tcatataact cctataatca
     5581 ccaatcattg ttctattacg ttccattgag cacccttaca ctataccaac attactgttt
     5641 acgactgcat cttggcaata cgttctgccg ggctcacaat atcggttaca cgaactccaa
     5701 aattttcatc aataacaaca acctcaccac gggcaatcaa tttaccattg acaagcaggt
     5761 caacaggttc acccgccagt ttatcaagct caatgatgga accttcgccc aaccccagta
     5821 tatcttttac aagctgccgt gttctaccca gctccaccgt aagcgtcatg ggtacatcaa
     5881 gtagcagatt aatattccct gttggaattg actgcatgcc ttcacccagt ggcgggaacc
     5941 gtactgggct tatacccacc tgtgaagttg ttctgggctg tgccggttca ggcctgtatt
     6001 ccggttgctg catttgcggt acataagcac cacgggcaat ctcacctgca agttcgatat
     6061 tcatcagatg atacattttg gagttgatta aattctggat agtaaggtta taggttacct
     6121 tcactacatc cggagttcct ggaacttcga tattaccctg aactactttt aaatcaggag
     6181 ctgtggtatt tacaccttta ccaattttat tgctcaattg tgttgctata gtggacatca
     6241 tctggctaac aaattcctgc aatgtactct gatgagcctc agttagagtt gcaggtggtg
     6301 tacctgaatc atctcccatc attaatgatg aaatgacacc ggcatcattc attgacagta
     6361 aaattacatt cttgccattt aacccaccag aaaaatttac tacaatctgt acatattgtt
     6421 tgggaaattc ggcacgcacc tggtcggcac tttttacttc caccagggca ttgccaataa
     6481 taatattctt acctaaatag cccgataatg ctggcattac actgcccatg ctgctattca
     6541 aaaaatcagc tactgattct ctttctactg gcgataaagc agcctgagca ggagcaaatt
     6601 cccctgctgg tacccttgca gtacctgaga gcatatcatc agcaccctgc aacagggcat
     6661 ctatttcatc ctgggacaat gaaccatcac ccattgtttc ctccagtgtg tttaattctt
     6721 acatatacaa ttctttgaaa aatacttacc ataattaata atacaaaata ttacactaat
     6781 ttacaacaaa ttctctgaaa tagacttctt tgaccttgcc ttttaaaagc aatacattaa
     6841 tgtgtgcttt tatctcttca gctaaattaa ccgtatcctc aacagaattc agatcttcat
     6901 aggatttatc acgtaaaagg atgttaacca catgctgcat ctggggagta cgctgaacca
     6961 gttcagcatt taattcaaca ttttcctcat atcctaatga aattgtaatc tttgcaaaat
     7021 gcggttctgg atcattggtt gttattgaaa atgatggcat atcaaaatga gccagcggtg
     7081 gtggaggtgg agcaagaact atatcctgtt ctcgctgata acgctgctcc tgaacatatt
     7141 tagcaacaag atacgaaatt ccaataatga taaatattgc cagtatagcg ccagctatat
     7201 agagcaggat ctttataata cgcgaagtct caaaagcacc tgtagccctg gcctcttccg
     7261 gcttttcctg ctcctctatt tcttcaacat tgacccgttc ttcatcaccc atgcgttata
     7321 cctcaccaac aatttacatt ttcatttagt attatatcaa taaaatctaa acaaacattg
     7381 ttggttaatt gttatactaa atttatcgac ataatactat attaattaaa aaagtcaatt
     7441 aatttatgat tatgtcacta aaatgtatca atttttttca atattttgta cttctccctt
     7501 tacatttctt attaacgcca ttcttcatca ggcaaaggcc ttacaatccc gggtgtagtt
     7561 ggttcaatta ctcctttatc cctgagtata actatctcaa ctctccggtt ataggctctg
     7621 ccttcaggtg tattattatc atctatgggc ctgtattcac caaaggcaac tgctgacatc
     7681 tgctttggat taacacctgt ttcctctatg agatatctca acacattaac cgctcgtgct
     7741 gaggaaagct cccaattagt agcataccct tcttcaagac ctcgtggagg aacaggtctg
     7801 ttatcagtat gaccttctat cctcacatag ttgggcattg actttataat cagggcaact
     7861 ttctttaaaa ttggtataat atcatcctta agttttgcac tccctggttc aaaataggca
     7921 tcacttgata atgtaataac caggccccgt tcgtcttcgc gcactcttac ctttttagcc
     7981 tgaatttctg gtttaaaagc ctctactgcc cttttcagca tctttgcaag agctcttccc
     8041 tgctcactgg aaggtaagtt taacatatta agacctatct cttccatcct gcctcgtgcc
     8101 agggaatatc cgccctgcat catccccaat gaacctttaa aagaagacag gacaagatta
     8161 aaatcctgtc caataatagg ggatatatca aacatcatga caaaaaacgt taaaagcagc
     8221 gtatttaaat caccccagct ggtaagccat ggtggtggtg gtggtagttc cattttcttt
     8281 ctttttggca tcagtcacct acctcactgg taatggcttc acgcaatctt ggatcaagga
     8341 atgataccaa tttatcttta acaattcgcg ggttatcacc tgactgtatt gacaatgtcc
     8401 cttctatcat aataagtttc atcaatactt cctggttatt aagaaaaccc aacttagatg
     8461 aaattggata gagtaccaga tacgccatga tagcaccata gaatgtggtg ataagagctg
     8521 cagacatacc aggcccaata gctgatttat cttctatatt aaccagcata agaacaagtc
     8581 ctaacagtgt accaatcata ccaaaggctg gagcatatgt tgcccagtca tcaaaaagct
     8641 ttttaccttc atcatggcgt gccataatgt tttcaagctc tgtttccata atgcggcgta
     8701 ccagttcagg gtcagtacca tcaacaacaa gctgtatgcc cttgcgtaaa aactggtcat
     8761 ccagttcatc cagatcatct tccagtgcaa gcaatccctc acggcgagct ttttcagaaa
     8821 aggaaacaag ggtaatgata agttcgtttg gattatactt tggtgggaaa aatgcatgtt
     8881 tggcaacttt ccatattccc agtaccttgc ttaatggatt ggatattaac aaagcacaaa
     8941 aggaaccgcc tatagtaata aatactgaag gtatatcaaa aaagagcaaa ggattacctt
     9001 tggcaaaaat tatccccata attatgttgg taaaaccaac aattaaacct ataacggtag
     9061 caagatccat atagcgttac tcctcctaaa gtagccattc aaatcgtaca ggtattctta
     9121 ttaacaattt tcggttaaat ttttaaaatt attcattaat tattatattt attattacta
     9181 ccagattttt tcccaggtac tatcgcccaa attatcattt tgtaattatt taaacccagt
     9241 gagcacctta tgataatatt cataaatacg ggataatacc tcatcttttg actctttaac
     9301 cagatatttt ctttcattgg tcagtgttat ggttgtatca ggcttcgact ctatagtttc
     9361 aatgtgattt gcattcaaaa tgaattcctc gccattcatt ttatgtaatg ttatcatgtt
     9421 ctgcctcttt tctttattgg tttatcagga tataatgtgt tcataacttt actaattgtt
     9481 actttagtga ctttattctc actgcaacca ggaatactgc aatcaaatac tggtatcgtt
     9541 acgtttgtaa aaaggttttc cagccattgc ctggcagcca gaaactgatg atctgtataa
     9601 tatatcgact ctcttcgttt gaccatgtac attttctttc tatgaatcat tgcataatca
     9661 tttgaattgg ctgttgaaaa tctattgtaa tgcatgataa agcgctggca tacaaccgta
     9721 tgctgtgcat agtaattatc cttaaatgca aggtcattgc ctaccattgt taacgaagca
     9781 atagaacacc ctaacgccag tgatattgcc tgtgttgtta caatacttgc tgttgctaca
     9841 tatcctaaat gttggcctgc aagcttccac aatgcatcaa aagggctttc atatatcatc
     9901 cagttataaa agctgatttt acccttccag gtagccaatg tatgtgagct tactccactg
     9961 aacgctaaca gatggcagga tgacgtgggt aaaaatgcat aatacgaaac aggcatggcc
    10021 tcgcatgata tagaaaaatg tggcttaatc ccagccagta ctaacggcct gtacgccata
    10081 tctgttgcaa taatagcaag gtctttcctg tattgatatt ttcttaacac tttaagtgca
    10141 tcgtttaaac tcctgcctgc accacacaca acaacatgct tgccacttaa aagcgctaca
    10201 accttctgca acccgcccca tttttctatc aatactttat ttttggttat attacgcttt
    10261 attaaggcat agcgctcctg taataacccc tgatttaatg cctgataaag gctttcagta
    10321 ttcataataa tggaaacctg atcagttaaa attgcatgct actgtattac ctggcgtatg
    10381 atagtatgct gcttcagtgc cccttcaatg ccagttgcta tgccaaaata cacctgtagc
    10441 tctccataat cgccatatcc tttaacaatg ccagcaaacc cttcaatatc agcccagtat
    10501 ccagcagtga tacgaatatg aagttcagaa ccaatactat atttaaaatc gttaagtgat
    10561 gtagaatcga tccatatatt gccatactca acaaacggtg tcaaccacat atccctgaac
    10621 ataagcggca gcatcctgta cccaacatct tttcgaaata aaggaatgcg atactcaata
    10681 ctgcctacag ccaacctgtt accataaaac atgtctttgg caaaacctcg catgctgtaa
    10741 gcatcctcat ctgatggtat gctctgactc tttccttttg cgtaacgccc tatattatag
    10801 ggatacagat agtacggtgc attgtgtgca taaccaccct tcagcataaa ggctaacaca
    10861 tcgttgtcaa agccacggaa aaaatagtac aaaccaccac gcaccttgga atatgaaata
    10921 tctgaagaaa gtgaagaatc gtaaatatcc gcatagcttt caaaaaccat gccgtcctca
    10981 ggacttaccg aaaagcttcc cgtatatgtt ccatcatgaa aaaacaccac atgtgagccc
    11041 gcaaggacaa tatcatgtgt aaaaacagta ttactataat attcactaac cattgttttt
    11101 tcataggtat aatacaaaag caactgatta taattataaa atttctgaaa aggaattgaa
    11161 atgcctgacg atactttacg tttcagggtc cttcgcatat aggcaacagt atcaggcggc
    11221 tcccatggga acgtatcatc aaaaataatc ccttcatctt tatatgaaat aaacaaatca
    11281 ggatacaaac ctgagtaaat ataatcaagg gcaagtgtca tcctcttctg taacaatgca
    11341 taatccagac ctaacgaata actgtgcctg tacagagtat cattacccga cgtattaaaa
    11401 ccaatatgat tgtcatattt gtccgggtat aactcttcac tatatacata tggtgcccac
    11461 catggtccaa tcacggaata gattggatta tatggttgtt gtaaaagttc tttatgttct
    11521 tcatactgct gtatggtaaa aggtatgggc gatagttcct gtgattggcc tgttgcatag
    11581 ggcatcagtg caacgttata accattggct tcgtatgaag aaaaaactat agttgtgcca
    11641 ttattgctta tatcagggaa aaaagcgcca ccaatgacat tgctgatacg agaaattttt
    11701 ttacttataa gatttatttc atacagatta aacacaccat ccctgtcaga agaaaaaata
    11761 actttctgcc catcaggatg aaatgtagga tgaatatcaa caaaggaatc attggttaaa
    11821 aggagcatct tttctgttgt aaaatcatac agtgctatat cactgaagcc attatgtaca
    11881 atactgaaga ctgctttgga gctatcgccc aacacccgca aatacgaaat ttgaaaaggc
    11941 gtattgtgta taaaatactt ctgtatggta aaagtagcat caagctgtac cagtgaatat
    12001 ttccctttat catagagtat tgccacataa ccagatttga atggttctat atattgaacg
    12061 cgcaaccctt ttgttttttg tgaatacttt gtatcgtaaa taaaaacatc cctgaacaaa
    12121 tcaaatgact ggtaatactc tacatcactc actatgatag ttttgttgct cacagcaaga
    12181 ctattgggca tgttaacgta tgccagacgt gaaaatgttt tattgtgagt attatatttt
    12241 acaagcgatg gtttttcttt atttgtcatg gcaataaaaa caatatcatt attataaaac
    12301 cgtggaaaac tggtataaaa gcctgtactg gttaacacag tataatcaga tatgccacgt
    12361 tttttgattt ctgcaatctg atcattcagg tcatttatta attctttctg ccatatttgc
    12421 cacaaagcta tcgctcgtgc tccatacaca tcattaaaat tcttttccat cagaaatggt
    12481 agaatattat ccgcattttc tttaaatatt tcaccaaaat ctttttcagg ataatttcgt
    12541 gtacagaact ctacaaaaag accaccatat aagtagggca ccatgcctgc aggccattcc
    12601 cgtgggaaat tacttgccag ggatatatcc ttgaggtttt ttgaatatac ttcctgccgt
    12661 aaaaccataa gggtgtaatt agcgttgagt ctaccccagg gttttcgttg tgattcctgt
    12721 aatacagggt atccctcaac agcccatact ggcatgagta tatttggaaa acatactctt
    12781 cccagggtat agcgtgtagc ttctggaatg ccaccaatca tatccatggt aagagtatgt
    12841 gtgtattcat gagtaaaaac catacgcagc cagtcgtcag tattggataa gcctgcatcc
    12901 tcaacatgtg gcattaccac aaaaagctgc accctgttat acggaaacgg cattgaaaat
    12961 ccatttgcca tatccgtagt atcacacaat acaacatctg ttcggaaaaa aggctgccag
    13021 tttatatttt ctttaagttt ttcatgaact tcttcagcaa tagtggccag tcgcaatgcc
    13081 tcatgctcta aaccatcatg ataatgaatc cagaaatggc tggttttaat ggaataccag
    13141 gtatacctcg gatgaaacat acttgcagca tgtaccgtat atgcaaagaa aaaagtaagc
    13201 aggatactat aaatccataa taatcgtttt gacatatagt gacaccttga atttactatt
    13261 ctatacgatt tatcaaaatt tataatggct tgatacaatt ctattgtatg aatgtacacc
    13321 cactgtaagt agaggagtgt atgatttatt attcataaaa tagttataca ttactgcaac
    13381 aacaaaatca taatggttta atcatggtag ttcctgattt cgtttgaaaa tttgtctacc
    13441 atttcaacca gtgattttga tgcatgggcg actctccaaa ttcatttttt atgattgtat
    13501 tataatctct ggacaaatca agattttttt acattaatta ccataatacg ttaacagttc
    13561 tttgtgttca tattgtacat ttattataat catgttgtta agttatatcc ggaatgaatt
    13621 taatactcat aaatatattg acattttgat tattattaca atatagtata gacaggacaa
    13681 tagcatatgc ataagtggca catagaaaaa cgcgacactg ttaataaaca aaggattttt
    13741 tcaattgaaa aacttttgtg ctatcatcct gcaaaaaaag ttaatcatga cttttacatt
    13801 ctcaataacc ctgactgggt aaatatcgtt actgttgaca cagacggcaa tattgtactt
    13861 gtgaagcagc accgattggg caccgatgaa tataccatag aaacagtagc aggccttatt
    13921 gaccctggcg aagacccata ccaatcagca catcgcgaat tattggaaga aactggctac
    13981 cgagcacagg aaatagtact actcaaaaag ctggcagcta atcctgccat tatgaataat
    14041 tatatctatt tttactgtgc attgaatgca cagaaggttc aggaacaatc gttagatctg
    14101 gcagaggata ttgaaatagt gacattatca ccacagcaag tattagagtt tatccagaaa
    14161 ggaaccataa atcattctat tattatcact gctcttttgc tttacttttt gtcaccctac
    14221 tcaacaattc caaaagacaa aatattactc tattgataca ctataatgat aaccggtaaa
    14281 ttgattatag tatcactatt tttattagtg caaagtactg caccggattt ttctattact
    14341 ctttcctatc accctgctcc attttactat gaggagtggc atattaccat agccaggatt
    14401 ggacaacacg catacattga gaccacaagc cctcagtatg caaggaaaaa acgttcaatc
    14461 ccgattgatg aatattcatc gcttgtggaa aaaattgttt catcaggaat atggaattgc
    14521 aaagactgct actcatatgg aaattattat atgctgacga ttactaaagg taactatcgc
    14581 cacaaatgca acattgagta tgcaccacaa aaaaattcct gcaacaatgc cactcagatt
    14641 atccgattta tcattaatac agccaataat tatttacagt aaaagacttc attaaatagt
    14701 gaaaaaaata aaatttcatt tgacaaagaa aagcatacaa taataaaatt tgtaatatat
    14761 tattcaatag catatactca caaaaaatac gaatcattaa tcttaaagga ggttatatga
    14821 aaataaagta tgtaatatgg ttattaccca tagtattagt tttttcttct ttaacatttt
    14881 cacagcaaac cacacaggaa aatgtggtcc agcctgatgt acagagtcct gcatcacctg
    14941 aagttaagaa agaagagata gcaacagctg cactcaaggc tgtatcattc aacaatggcg
    15001 tgaacacttt tgtaaattca aaggttaaat ttgaactgtt ttcaaaagac aatgtagcaa
    15061 cggataaaat attgtataaa cttgatgcag gtaatgaact ggaatacaaa gagccttttg
    15121 ctatctatga agaaggcaag catgttatca gctattatgc tattgataaa gttgggaaca
    15181 aagaagaccc aaaagctttc aaggtagttg ttgataacac tccgcctgta gttaatgtct
    15241 ctgtatctgc acctgttatt aaatccgggg acaagttata tgcttcaaaa aattttactt
    15301 ttgttgttga agcaaaagat gcattatcag gggttcaatc attagaatac agtatagaca
    15361 aaaccaatta tcagccctat ctgacacctt ttaccatacc agttgaaggg aatataagct
    15421 taagcattaa agcaatggat aatgtaacca attatactga aaaatataca atggttgcaa
    15481 aagatgaaaa tggcaatccc ataaacttaa ctgatggtaa cgtatcgata tttgttgaca
    15541 ataaagcacc tgttgtagaa attaagcccg ataaagaact gcttgataaa aatggcagaa
    15601 aagttgctac agcggattac agctacacaa tttcagcaag cgatgctgaa tcaggattat
    15661 cctctattct tttccgattg gatggcaaag gcgaattcaa agcatatact ggagctataa
    15721 agtttgatac caacggtgag cacatgatag aggcaaaagc agtggataaa gttggcaatg
    15781 tatcacctgt tgccgtatta tcagtatttg ttgacatcat tgcacctgat acgacaataa
    15841 aagctattga ataaattttt taactttacc agcactgtaa taaataatta cagtgctggt
    15901 aatattataa ttataatact atttatttta ttataatttg aaagaatagt aaatatgttt
    15961 attgaaacaa caaaattaga ttcaattctt gtgataaccc cacacgaaga tattatcaga
    16021 gaaaactccg aagatttaga aagggctatt tttaccaaca taaacaaatc aactactgat
    16081 gttatactgg actgtaaaca tgtgcagtat attaacagtt tttcattggg cacactggtt
    16141 aaaattcacc agaagcttga agaaaattcc cgcaaattac atttcctcaa tgttgcacca
    16201 tcaataaaaa cattgtttaa aattacccgt gtactggatt acttttcact tattgactca
    16261 atggataatg ttaaaaataa aaagtagctt tttataacaa aacagattct tcaccctgca
    16321 ttacaagttt cagaataaaa gacctgtatc ttttttattt ttttcaaaaa aatatttatc
    16381 ataataaact tttgatttat aggtatcccc agtaatttca taaataacag caatattata
    16441 aagcgattca acaaatcctg tatctaatct ataagcttcc ttaaagcaaa aatttgcctt
    16501 aacatagttt ttagcaaaaa aataggcaaa acctaaataa aaccatccac gtgcagatgt
    16561 tgcatccaga gttacagcaa tatccagatg tttaatagcg ttattaatat ctcctgaata
    16621 caggcacgct ttcccatatc cataatgggc aacatagtaa taaggattta aaccaatagc
    16681 tttgataaac aattcttttg cttttgaata ttctttagct tttgcaagtg cagttgcttc
    16741 agtgcatact gaggaagcaa actggctttg aagaggaaca aaacataata acgcaatgag
    16801 tattgcttta ctgagcattg gggatcattt cgcgaagtgt atgaaataaa aatgtgtcaa
    16861 tttgtttact gtaaaatgta tacaatgtga aaacaagcca tacaacaagg gtaaatatac
    16921 acataaattt cagtaaccgc tctgcaccaa tgtcatctgc cttgtaaaag ataattatta
    16981 acgctaatgg ccataagaaa aacatatttg caaaaatcag ggcactgaat tcttttataa
    17041 aagcgatagt taccagatac cagtcttttt caaattgttt ataacctttt acaagatctt
    17101 tataaaaaaa ccacacataa aacccatata taccagaagc agcaattttc catattagtt
    17161 caattctggg acgcatccat aatagcagca cacacagccc ccagattgtt gtaaatatca
    17221 ttaacgaagt ggaaagcatt gttgtattca ttgggtatta ttcctctttt aacgctaaga
    17281 aactagccaa ttctatggct ttcttcatgc tgctatagtc agcacaatct ttgccagcaa
    17341 tatcaaatgc agtgccatga tcaactgaag tccgtataat ggataatccc acagtcacat
    17401 ttacaccttc atcaaacgcc agcatcttga aaggtattaa gccctggtca tggtaatgag
    17461 ctataataca atcatattgt tcccagcgct gtggtataaa tatagtatcg gcagaaaatg
    17521 gcccttcaat tatgattcct tgtgcttgtg catcctttac aacttttgca gtgacatcct
    17581 tatcaaaatt gcctattgca ccattatccc cacaatgtgg atcaagccca catatagcaa
    17641 tctttggctt ttttttcatt attctggtta agctgctttc acaaatctga atagtttcca
    17701 tcaatacagc ataatcaata acatctttta gtttgaaaac aggcacatgt gttgtaacaa
    17761 gcataacatt atattttcct gaatacatga gcattcgtgg gttatcttca tgtatctgtt
    17821 ctgcaatata ttcagtatgt cccataaatg gtacccactt ctctataagg gatttattga
    17881 caggaccagt aacaattgca tcaatcatct ttagcttcca taacgtaatg gctgcatcaa
    17941 tatatgctaa gctttccttg cctgttagtt cagtaccaca ggaaggttgt gggattggta
    18001 catcactatg gatatttata aaataaggtt tctgtataac agtatcacgc tgtatttcat
    18061 taaaattcag tatatcccac gagggcatga gatggtgata gtattgttgc aatacgccaa
    18121 ccctgcatat caccagagga aggcacggca gtgtttcaaa atcgcataat gcttttaatg
    18181 ttacttccgg tccaatgcca gctggatcgc caatagtaat agctactctg ggcttcctaa
    18241 tctgcaagaa actcctcggg tattaattta tttttttcat attcattttc aatcaagtct
    18301 ttggcggaaa tcttaagatc atttgctata gtgcagcgcc cttctaatat tacaccattc
    18361 tggattatca gttcaggtgt ttttatatcc cctaatattt ttgctgtggg cattaacaat
    18421 accctgttgg tagcattaat attacctata accagacctt caataataac tgatacagca
    18481 ttaatattgg ttttaacttt tccgtttggg ccaatatata gctggtcagc ctgcaaatat
    18541 ttcccttcaa aacgaccatc tattcgcaat gaaccgttaa taagaaattt gccactgaaa
    18601 taagaatttt caccaataac attatttgca acatcaccac cagattttgc taaagccata
    18661 aaaacacctg cccttcattt tttttcatag ctacataatg aatatattta tgtcaacatg
    18721 aaaattgtat ttacattcat gatttgtata caccgccgcc tgtgcggtat gcatatatac
    18781 agttatgaaa ttttcaatac taaaactttg ttttatcagg tactttaatt atctgtaata
    18841 cttcctgaac aaaccctttg agatatcgca atgcttcttt tactgtcaaa ccttcaaatg
    18901 ttttatacat atcatacctg gcgtatgtta ttttgtcatc tgcatttaaa accattgtgc
    18961 caccaaattc cgtatctgca agtattttgg ttacaaaatc attaacatct ttaaaaaaag
    19021 aaaataaatc cattaaaaat aacctgccct gccgcatgcg cataagattc aattgtgaaa
    19081 tatgagcgct tgtatttgcc ttttcaatat catccactgt ttgctgaaaa atctttaatg
    19141 ccctctggca ctcatctaat tttctgtaca gagaatctcg ttccagtgaa ttggaataat
    19201 caagcttggt atattgatca gctatcacac cccatatttt ttctgaaaaa tgctttaaaa
    19261 ttgaagcagc aatattaatt tcattatcat ccttgaaatt tgatattttt tcaatttcaa
    19321 cattttttaa atactccagg gtttccagtt ccgatttccc ggttggggtt actattaaat
    19381 catcagttaa tattgcttca ataagttcat caagttccac atcaggatat aactcttgaa
    19441 gtttatccct cagcgatttc agatcttttt ttacccgctc acgctgtttt gcatcaggtg
    19501 aagtttttaa tacaccttct aacgcccgta tagatcgtac tatctgttta gctttttctt
    19561 ctctctcgtc cataatcatt cattacatta ttttctactg cattttaagc tatagtatca
    19621 aacttgatat ttttttgcaa cataaattcc tgctacgtga taagctgaac agtaataatg
    19681 aaaatttaat cgtcaagaat accctgttac taccgatagt ataagtaaaa taaaacatta
    19741 tgataacatt atatatggaa tttgtaaaaa aatattattt attacatctt tttatattat
    19801 tatgtcttat tgttgcacca ttagaaagtg ctccagctgc tataatacct gttatcaatg
    19861 atctttcccg aagcaatgat actattacgc tgttacgtaa ggatatacgc aaaacactgt
    19921 acactataaa aagcaataga tcggttgaag aattacccga gcttgttatg tataaatata
    19981 tagttaaaaa aaatgaaaca ttctgggata tagtatctat tacaaatctt gatcttgata
    20041 cgatagtttc tgttaattca ctttcagctc catcagatat aaagaaaggg cttattcttt
    20101 atataccgaa tatgcgtggc ataatacatg aaaaatctca aaatgaaagt tatgatacca
    20161 ttgctatgag ctatcgaata ccggtacaat atattctaaa agttaacaga atagttacta
    20221 acgaacataa acaatatata tttattccgt gtgggaccct atctgattta gaccgctccc
    20281 ttttccttgg aacagctttt gtttcaccac taacggtagg caagctcagt tcttattttg
    20341 gcgtgcgtaa agatccaata gaccatgcac ataaatttca tgcaggtgta gatattgcat
    20401 gccccataaa ttcacctgtc tatgcatctc gagatggtgt ggttgtattt tccgggctcc
    20461 tgggaggcta tggtaatctg gtaattataa aacatagcag agactatttt acattatatg
    20521 gtcatttaaa taaaatactt gttcaaaagg gggcgatagt tactgcagga cagcttattg
    20581 caaaatcagg aaacactgga aggactacag gtcctcacct acactttgaa ataaggaaaa
    20641 acagtaaacc aataaatcca ttattattgc tacgataaac taatcaattg catcaagcag
    20701 cgaatataca tctgtaccgt cttcaggata ttcagccatc agaaaagtaa ccagcaattg
    20761 taattcttta tcactgaatt gctgtaccat ctcattttta attgcatttg caaacggtac
    20821 tgcaaatttc ttattcttac cgggcaacac aattaaaaat ttatttctat caatccttgc
    20881 agtaaaatca ccatcagaga gtcggctgat gataactgct tcaattttat tcataagttc
    20941 ctgggttttt tctaacccct gaattgtata gtaccgcttt aaatttttaa ttgtaaagag
    21001 tattatggta agaggaattg ttaatctggt aacattagcc aattctttat caattctgtt
    21061 aaaaacaggt aatatgacat cactataatt aacatattga gattcaatta attgcaatgt
    21121 cagataatga ctcagtaaaa catctgtcat tactttgaac cgttcaaact gaatttccct
    21181 gcgctggtag tcacgcagcc tgaagacaca gaaaaaaccc agcatctcat tagcaacttt
    21241 aaatggatta atccagaata atgtcatctt gcgaatactg ctttctgaaa aaacctccct
    21301 tattgccctg tttctgtcaa aatcttctaa gtctgcagct gaatgtaagg ataatatata
    21361 gtcaataaac gctgaatcac tattaataga atttgcagaa tctttaagaa gcagcatatc
    21421 ttcctcatct gtcaacaata aggaatacct tccatcctta ttatttttaa tatacactgc
    21481 ataacttaag aaaccatttg ccttcataat actgtgggca cttaccttga aatgatcttt
    21541 ttgtgggtac tttcgtaaac tctgtttata ttcatccagt tcgttccaga attttctgtc
    21601 gtctactgat ttgcgtatat cattttccaa ctgttccatc ttatgcactt tctcataggt
    21661 tatggcagca aattcattta tccgttcaat aaaatcaatt tcctcttcac tataatcccc
    21721 aattgttatc ttttcaccaa gaaccagagc accaaagacc tttgaatcat accgtaaggg
    21781 aataaacaaa cgagcatcaa tagcaattaa ctgataatat tcatccctat attgggtttc
    21841 ctgtttaaac tgttctatat ccacaactcc tttagattgc ataagtatat taaaaaggcc
    21901 ttttgtaata tcaaatcgta tatttttatt ttttattgtt attcccctgg aatcagccac
    21961 aattaaagaa ttctcatcat cagcgcttac taccagtaat gtagcagatg cagatccaat
    22021 ctgacccata acagagaaaa ggatggcatc atacaattca ttgagcgaat gagctttaat
    22081 gatttcttta ccaatttcat ataaaacaac aaagtcttga aacttcttat caggctttat
    22141 ggaatactca gtagaaatag tttgttttgt ggtatcacct tcctgcatca cacgcatact
    22201 atccttattg tcttccatgg attcaataat tctgaatttt gggttaccat ctgatctatt
    22261 atcaagatct ttatctttaa attcgtttgt ttcctgaatg ctgttattct gtaataattt
    22321 taaatccggt tcatcttcag gacctaaata atcgggctca ctatttaaaa taagttcctg
    22381 gtcttgcatt attatggtat cataattagt tgtatctgtc tcaactttat cttttgctgg
    22441 agtattatca tcaggtaaaa taaataactc atcttcgatg ggctcaatac ttttcagatc
    22501 cccattactc aattcaatgg tatttccatc atcctgtaaa gctgtcgatg catcatcttt
    22561 tctgtcatta ataaattcag tctctgcagg tccaataatt gtatctatca gtgtttcttt
    22621 gcccttttta tttatttcct gtttatactg taatgctttc tccagtaacc ccatacaggt
    22681 taaactccta attcatttaa taccttaacg tacaggtcaa aatattcaga attggcaaaa
    22741 ttctttataa catcttctgg taatttctca aacaaggaat ccagatattt aagtaacctt
    22801 cgaatatttt cctgcttctc aactggtaat gtatcaacaa acctgtttac atcctctttg
    22861 ctatcaagaa taatgacctg atcggtaata ttagtaattt cttcctgttt tgcaggtttt
    22921 tcttcaacaa cggtatctga tttactttca tctacatcat atagctttgc tggttggtgt
    22981 ggcatttgag ccaataattt cttaatatca acctgaggtt taatcttttt taaccgctca
    23041 actatatcat caatattttc ttctatgaca acagctaccg gtgattcaat attctcttca
    23101 atacttttct tttctttatc agacaggctt tgcggttttg cctgtatata tttaaattgc
    23161 tttactaatg tagcatcctt ttcagggtgg tctctcccaa ctgtaacatc tttaaatgct
    23221 tttgaagctt taattgtttc atactctttt tcaaaaagaa tgtcataaaa aaactcaaat
    23281 tcatcttttg ttaatccaaa aagttcaact gtagatgaac tctttttaac tgtttttgtt
    23341 tcataataat catcaataac ctttaaatac ttatcatatt catctacaag aacagcattg
    23401 tctataaaaa ctatatcatc atctttaaac ttatactctt cttcaaactc tatcattcgg
    23461 tcctggaatg ttggcaatgt tcctgtcagg ataggagcta tatattcatc ctcttctttt
    23521 gaacttgctt cctttaatat tttagcctct ccttcagtaa tttctatcat gccactgacc
    23581 aacttatcaa tatcatctat agggttttct tccattgata agtgtgcttt aacctccaca
    23641 ccagcatcat ctatgatcat aattttctga ttagctgccc ctgccaggtc atcaggaata
    23701 gtctcaatgt taaattgtgc ataatctctt attaaatttt ttgtatcaaa tgtatcatca
    23761 gttgtttttg tagttactga tgcaggcaca aattcttttt cttcttctaa tgttattatg
    23821 tctatattct tttcatggat tgctggttct ggtttatctt gaggcttctc ttttacttct
    23881 gtatctattt ggggagcttc tgtgattatc acttcagtca caggaatttc ttcctgcttt
    23941 agatctatac tctcttcttt ttgtataatt tcctcttgta tgggttttac aggctcaggc
    24001 tttttctttt ctttatcagg tttaatgata gcctcttgtg aaagctctac atattgtggc
    24061 ttaacatcaa ctacttcttt tttttctggt tcatgtttct ttggggaaac tttcacatct
    24121 gaagttactt tttctttctt cttttctttt gatttttttt cttctccatt agtttcaata
    24181 ggaataagat catattcatc aagttcttca attaaatcgt cctctgaaag aatcaaaata
    24241 tcttcactag ctatctgttc agcttcatca agatttataa attggaaatc ttcaatatcc
    24301 ttttcctcta ttttatttct aacttcagga ggtatctcaa tagttataac atctgcttct
    24361 ggtttattaa ttttactttc atcaccaatc acattatctt catctggacg taaaggttta
    24421 tcatgcgcat tttcttcttc aaattcatat ccagaagttg cttcttttaa ttcttcctgt
    24481 tctttttgtg ctaattcctc atcttcagaa aaatcttcta ttaccagtgc atctatattt
    24541 ttatcataat atatcttacc ttttatttgc ctgttttctt catcaatagt agtatcaata
    24601 taactatctt taatatgtga ttcttctttt ggggatttag aggaatgtaa aggttcaggg
    24661 atatcctcaa acaacctatc ttctttctga agcttcccat gaactgcagt gtgtttcact
    24721 actttaattg tattaatagc atcaacagtt gcggttatac tgataatatc atcatcacca
    24781 cgtttctgta tttgcaaagg tggattttca tcttctataa tgagcgtcga agtagtaata
    24841 tcctgtaagc ctgtaatctc ctccagctca cctgaaacct tctcctctgc aggcatatca
    24901 ataataacat cttcctctgt taaatcaata acaggttcag gtatgtctgt aatttcttcc
    24961 agctcacgaa ctttatgttc caattcctga ctcatatcca ttatggtttt ttacattaat
    25021 tttatgaact atctcatagt attaatatat agtaatattc acaaaaaatc taattgtgcc
    25081 agtaaaaaca attatcatat taatttatct gtaataatta atatgatttt gtcaatagtt
    25141 atataaaacc cttactgtac aatatcggtt tattttgtat tattattgag tagcaatgca
    25201 attactatat tttttcaaaa aatcgttgca ctttaatgtt attaactggt actatctcac
    25261 aatacactat gtaaaaataa ttaaggagga aatactttta tgaagttaac acacaagtta
    25321 ctttgtgtgg cgatagctat agtgctgttg ccaacaacat cgtttgccat agttgatctt
    25381 ggtatgtatg gtggatatac atttgccggg aagtttgatg ctccaactat cagcaatgcc
    25441 gacacaaatg gctggcaata tggttttatt ggccacctca atggttcggt aatcccgatg
    25501 atattaagca tgggaattgg tggattttat caaaaatc
//
LOCUS       DAORYL010000041        25399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1639_length_25399_cov_12.784643, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000041 DAORYL010000000
VERSION     DAORYL010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 25399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25399
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1639_length_25399_cov_12.784643"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PLV81_08745"
     CDS             <1..690
                     /locus_tag="PLV81_08745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOM10222.1"
                     /translation="ADVIVAQSSNTRNNAIEYYAPKKEIQIIPLAFHPPIIPQTSRKI
                     LGLHEKDFICITIGRLIKRKSIDVLIRAIALVNKPAVKLLIMGDGPEREYLEELVNKL
                     SLQDRVKLLGFVGDEEKFAYLSQSDLFVLTSMHEGFGIVFMEAMYCGLPIVATNHGGQ
                     IDFLSHEQNALLINVGDTKGCAESIMRFYNDKKLYTRCSVNNKKKISTFYAENVAGRY
                     MNIFLSLIDRK"
     gene            687..1334
                     /locus_tag="PLV81_08750"
     CDS             687..1334
                     /locus_tag="PLV81_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOM10223.1"
                     /translation="MKIKTALITQNKELVQEVRMLQILDVSVHGTINTLKDEGFDIAL
                     FDTEQIDISDAQFYLSKLRKRFQQLPVILIVRAGYLESINRDWFFDDFIVFPFRKGEL
                     KTRIDRLIGSEIELKESVIKVGNITIEPDKYSVTVNNEKVVLTYKEFELLKLLMENKG
                     VVFTRQELLSQIWGVEYIGGSRTVDVHIRRLRIKLGDEFNNIIETIRNVGYKCKE"
     gene            1427..1921
                     /locus_tag="PLV81_08755"
     CDS             1427..1921
                     /locus_tag="PLV81_08755"
                     /inference="COORDINATES: protein motif:HMM:NF015817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HOM10224.1"
                     /translation="MNQLKKIEKVLESALHEDLTPYFALVKAMRKHLPLFGSTKRITA
                     ALLKECIGDISKLDIAEVASLNFTKTSDGTIIFEDIKDGKARIFINIGKNHKITPGDL
                     IREISKRSGIDGKLVGKIDIHSTYSFIEIPEQYAELVLLSLDKARIKGVPIVVEPAKK
                     KKKQ"
     gene            2010..2735
                     /locus_tag="PLV81_08760"
     CDS             2010..2735
                     /locus_tag="PLV81_08760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOM10225.1"
                     /translation="MYNASHSPAVYSTDYFGQEYKAQYGKTYEDDFESIYAASLFRLS
                     IIKKYLGNNVSAKSLLDIGCALGFFCKAARDVGFGEVEGLEISEYAVSYCKLKFNIPV
                     HMTGFEDFIPVKKYDVITAWFVLEHFTDPYAMFYKIYGMLEDNGIFACTLPSYFGPLF
                     YCHRSEWIRSHPQDHRIDVAPWSVKKLFQQIGFFSVRCFPSGYHPERLGTIAQKYKKL
                     YNTIANLLCFSDTILVIASKKPQ"
     gene            2911..3069
                     /gene="rpmH"
                     /locus_tag="PLV81_08765"
     CDS             2911..3069
                     /gene="rpmH"
                     /locus_tag="PLV81_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOM10226.1"
                     /translation="MKRTFQPSVIKRLRTHGFRARMSTRAGQEVLRRRRQKGRYKLTV
                     SDEKRNSK"
     gene            3092..3457
                     /gene="rnpA"
                     /locus_tag="PLV81_08770"
     CDS             3092..3457
                     /gene="rnpA"
                     /locus_tag="PLV81_08770"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOM10227.1"
                     /translation="MKRRYSLKGLKRFKEVIANGKRVYGKGMRMIVYMKPLQAEGNMN
                     NILQSRFAIVVTKTYGNAVERNRIKRQIRSILVTLMPQMRQGFAVIIFPDTTCKAMQY
                     NHLVVSVKQVLLKSGVLKQ"
     gene            3466..3675
                     /gene="yidD"
                     /locus_tag="PLV81_08775"
     CDS             3466..3675
                     /gene="yidD"
                     /locus_tag="PLV81_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006625455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOM10228.1"
                     /translation="MVTIPVVLIKLYKLVISPVLPNACRFYPTCSQYAIEALTRHGLF
                     KGGFLSIKRILRCHPFCDGGYDPVP"
     gene            3791..5413
                     /gene="yidC"
                     /locus_tag="PLV81_08780"
     CDS             3791..5413
                     /gene="yidC"
                     /locus_tag="PLV81_08780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOM10229.1"
                     /translation="MEKRALIAVLLSLGIWILWFYLFPPQEKTPPVPEKSAETATVQT
                     EEIQKPEISVVSKFQQTFTKKEEVTIDAKYYTAVLSNEDGQCTSFIDKVRNIELIVTK
                     SKFDAKGFFDFPVHFSTGEFLHAKDIPPALWKMQKDASAVRFSTVAIVQKNPLEISKK
                     FTYNETGQYFVVSYAITNKGQGEFTFPEGGVIFSPSDFLGPSMDFDNSYNELHGIYYI
                     NDSFEKARKGGGFFSAPEDLKRENGVVQWAGIMSRYYLLIMIPQGFAGSGVVYDNRKH
                     TGFRTGIVVPVKPLKPGETITHEFKVYVGPKNKDKLLAVDKHIGDAADVSKWIEPIRD
                     FMMWCLLEINRLIGNLGWSLVIFSVITKIMFLPLTQRSTESMKKMQELNPVIQELREK
                     YKDKPDVLNKKIMEVYKKNKVNPMGGCLPLLLQMPFFFALYSALINSVDLWQAPFILW
                     IKDLSLPDTIYRIAGFNINILPLVMTGTTYLQQKMSSSEVTQQQKMLMLMPLIFIVIF
                     WNMPSGLVLYWIMQNVLQIGHQMLINKIGKKK"
     gene            5683..6321
                     /gene="jag"
                     /locus_tag="PLV81_08785"
     CDS             5683..6321
                     /gene="jag"
                     /locus_tag="PLV81_08785"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOM10230.1"
                     /translation="MKVLDIEGKTVEEATKKALSLLKINDMNKINIEVLDEGKSGIFG
                     FGISRPAKIRVYYQQEDDIGQQAKEIIEKILMLMEVDAKVKDYKESENKVYVELESSS
                     SGLIIGKKGKTLEALQFMVNLLVNKMTNSEKKIILDIESYRAKREKALRKLSKEIALK
                     VARTGKPWTLEPMNPFERRLIHLTLQNDSKVTTKSEGQGIYRKVKIMPVEKR"
     gene            6327..7688
                     /gene="mnmE"
                     /locus_tag="PLV81_08790"
     CDS             6327..7688
                     /gene="mnmE"
                     /locus_tag="PLV81_08790"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOM10231.1"
                     /translation="MDDTICAPATPPVHSPIAIIRISGPDSLRVASSIFRAQQHTQEF
                     IPRYAYYGIIHDNNEEIDDCIVIYYKHPQSYTGEDMVEIFCHGNPIIVNKIINCIVAL
                     NVRIAEPGEFTKRAFLHGKMDLTEAEAINHIIAARSEWEITTAIQQKHGSLKNAINDI
                     KQHIIELKADIECAIDFVDEDIEIISFENAQQRMHTIINKIQDILNRCRIGQHLSHGI
                     DIAIVGKPNAGKSSFLNCILNHERAIVSDTPGTTRDVIKESVSIKGIQCNLLDTAGIH
                     KKADGIELLGVQRSYRLIDEVSLIIFIIDAVTGMTSDDEHILELIKEKPFIPVINKTD
                     IAPKESIAIIQDAIGQKGIVFSAKTGYGIPALEDAVYQCIRSQYVEYKNSFIADIRMI
                     QLLEQSLVYAQEALHQMAISSPHEIVASALQNVIDTIGAVTGEISVDDVLNSIFSRFC
                     IGK"
     gene            7701..10379
                     /locus_tag="PLV81_08795"
     CDS             7701..10379
                     /locus_tag="PLV81_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10232.1"
                     /translation="MNKLLIITTIILVAIVPVKALPDVSEDSKRIYCNGNSLSIIEQY
                     EKEIVSGKAYPALILADIHHILQVAGINKTLMLLKELSRSADTDAERLYVQDIVNVIE
                     ELNQKDITKDINRWDIYDNIKERMPRSITSLQCTNNACFLSGKNKIRVSQYHSGFIPV
                     KQFTQDYNTIGLCSGSFKATVPVTLRIYSNMDYVCYINGKKICVNALTDKKKLLRMFH
                     IESEEGFTVALYYKPVENMFLKIQFYDDTNQIIHLPDTENTFYHDVQWYESYYEYENQ
                     LLTQYNNQRSGEAAFQLALFYESLQSIEALKYYKEALQSKNEFIACRFLGFLLEHENK
                     VPGSDVVVQTVLKKNNEKYTSVLWQNYYDYIYQRKSLDPFQVNYLPLLTHLLHREKDV
                     LIKKGVNLEPLLEQYPFSNDLSYIVAKITAYHDITKAISVLESIQEPDDKEIDLLIQW
                     YEQNEQHESIVSIVENNNIDRYFDNYIHALIALKRYNDAKSALFKRIAQGFDSHAYAL
                     LATIADLEDSDGSMYRQKHEVIVSGIPWHSDYMKSVFDDYVNKTVFSRLAFETIHRKT
                     FGLLYSCYALRIIDNTAYCSLYDLYYANSLSQINDYTFSKDVTITGCTIYLVSENGKI
                     EKKEIKNIDNSFQKGIKACFDENQNTYVLVELSFMIHKKLPVIHIQQNYNLSQFDMDI
                     IVIDPQGNPTITSVMKGNITKGENGIQHIRFYADELSTHHNRNFMVIALTPENLIEWI
                     NEQSVRFKIADYFVTDTELNGKAVEDKVAELMGKLKQYTIHPNPYTSTSFLQFVGLGK
                     GTQLDAILYSRYMLAKNGIMSYIALGCSSSNKFTEKLNKDNGLFFDTVLLYIPLSTEK
                     GIWFTCDGAYPSSDKININNILLVVGDEIILKSKNN"
     gene            complement(10549..11244)
                     /locus_tag="PLV81_08800"
     CDS             complement(10549..11244)
                     /locus_tag="PLV81_08800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOM10233.1"
                     /translation="MNIRGIQKTSLIDFPQVISTVLFTGGCNLRCLYCHNASLACNDC
                     NLQQYSNHEVLEFLQKRKHLIGGVVITGGEPTLAKGIVTFLEKVKETGLKVKLDTNGF
                     QPKVLKKLLSQQLIDYVAIDIKTSPEKYPVLTNTKLPFDLIMESIALLKEHHITFELR
                     TTCVPGFVTIEDLQSIKERVGTVKYYYLQQFHNQHTLDESLHTVKPYPKDILYSFQQF
                     VKTFATFCEIRGI"
     gene            complement(11207..13516)
                     /locus_tag="PLV81_08805"
                     /pseudo
     CDS             complement(11207..13516)
                     /locus_tag="PLV81_08805"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
     gene            13793..14314
                     /locus_tag="PLV81_08810"
     CDS             13793..14314
                     /locus_tag="PLV81_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10234.1"
                     /translation="MRINNVAIVIYLSVIMALGMAMGMVYSDDNSLFSTLAITPEDVP
                     QGFIFGKIPNFAKKVFTGNPCIVNSDGIRFLASKLYPEGNAANIKGMYVAIMASESRP
                     YGDDLVYYLLVFKDSKAASVEIKKLQDYYQYNKDRIVLITKDNIALLLFADDVDNYKY
                     IAELGQKLQERLN"
     gene            complement(14311..14715)
                     /locus_tag="PLV81_08815"
     CDS             complement(14311..14715)
                     /locus_tag="PLV81_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10235.1"
                     /translation="MELFDGFIAAIASEFQLKFHKTKEGAYTTTIEFENDRSQEILIT
                     LNKDESGDRIVNYYSVIGTIKNESFELYKFALKLNSTLDYGALALLNDTLILRNSLLL
                     KDCDPQRFMKSLFYIAAKADELEEMLITKNIY"
     gene            complement(14742..15335)
                     /locus_tag="PLV81_08820"
     CDS             complement(14742..15335)
                     /locus_tag="PLV81_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10236.1"
                     /translation="MRITNTIPVVPQASLATHRKASIQIQQPVESVRVYPSLKKAISA
                     YTIISEAHQLVTMAINISYELIQKAFTPQDHNEILAKIATINNYFTRLSPGSASVPNI
                     PQSDTLQETTKLIEQLGNAAKNNDTASMKAIHETLTSHAQILDNTQKQMYMALSHPAG
                     TDTPDINKIKSAIEQNQNMVFYAHKPLQYEHIQKLLA"
     gene            15787..15984
                     /locus_tag="PLV81_08825"
     CDS             15787..15984
                     /locus_tag="PLV81_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10237.1"
                     /translation="MKKLVIILCCICMISSISCKKKEENRIPREQIQIDGAHDYSLIF
                     KDIIDKEKKEQQRYKKGPFDE"
     gene            15977..16639
                     /locus_tag="PLV81_08830"
     CDS             15977..16639
                     /locus_tag="PLV81_08830"
                     /inference="COORDINATES: protein motif:HMM:NF023757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-J related domain-containing protein"
                     /protein_id="HOM10238.1"
                     /translation="MNKLIEIPKESLIAYCLNIPRPVMESVIIREIVNPLCNGHSTLY
                     QKHFSLFHALYNIKNNPDYFNYMVHIHPMQIVIRNYPVNNKCIDYNPLTDSFCGNQTV
                     DGTYCPFHYTFHAPFRGDMIYDPLNEFYSDPYNINYEFSDELKKVYSGFTKYIINRRK
                     VEQALSFFSFSAMEHCNKALLQKRYRELVKKYHPDHYQGDTTMIKEINAHYTLLKEIF
                     VV"
     gene            16802..17947
                     /locus_tag="PLV81_08835"
     CDS             16802..17947
                     /locus_tag="PLV81_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOM10239.1"
                     /translation="MKRRAFQIIPVIVLISVVLFVFNNFSCSNSGPSAFGNSSSSPLK
                     GDQETARAIEIQDTFRKIYELYKDRVVFITTEQVVELPPNPFFDDPFFRQFFGVPEQK
                     SRKQKRSGLGSGFVLSSDGYICTNYHVIANVDSVKVKVKNKDYKATVVGYDQRTDLAL
                     LKINPDEKLEPVFLGDSDQVKVGDWAIAIGNPFGLDRTFTVGVISATSRKDVDMMGGS
                     QSHIQTDAAINPGNSGGPLVNINGEVIGINRMIYSQSGGYMGIGFAIPINTAKAVLEQ
                     LKKYKKVKTGYIGVQIVPLTEEYANELGLKNTEGALVGGIMEDSPAEKAGIQPGDVIL
                     SVDEKPIKTYEDLVEIVSKTPIGKTLKIGVWRNKSKIYLFVTVKERP"
     gene            18000..18209
                     /locus_tag="PLV81_08840"
     CDS             18000..18209
                     /locus_tag="PLV81_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10240.1"
                     /translation="MDNKYRYIVIIAGIVAIVYIVFRYIIPLLFKLLGIIVSFVAYSI
                     AIVIAIAIVILIIGYVARIYKTHTM"
     gene            18551..20953
                     /locus_tag="PLV81_08845"
     CDS             18551..20953
                     /locus_tag="PLV81_08845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOM10241.1"
                     /translation="MKEQIKIWLDKTKNIINVYINDVRTWFYGIIAVSIIGGLLFGYI
                     VAQVQNCLGIDNLKRFQPSVPTKLYDVNGELIAELFQEKRDLVSFDELPKTLIKAFVA
                     TEDQDFYAHIGISPMAMARAMVKNIISFKIVQGGSTITQQLAKRLFTSGERTFTRKAL
                     ELVLTLQIEKRFTKDEILEMYFNQIYLGHGCYGIASAAELFFNKEVRFLSLAEGSVLA
                     ALPSAPGRYSPFMNTRNAYYKNWDILNRMVDCGFLTKQKAETVYTSFWPQYVDAIKTE
                     FPTKTAYSRIIDNAPFFTDYVRQILISRYGKDVVYNEGLQVYTTLNLKRQRAGEKYLT
                     EGLIKQNEVSARANQYMSGAVDRGLFSVYQQLGMLFNLPRVIVKSDTETIFKKFMVDN
                     LLDEMELVSLLCNADAMHNDIEKFHVVTSGISTSLKVEGALIAVEPKTGYITTMIGGS
                     EFAVSNQYNRAIQARRQPGSAFKPFVYGAGIESKLINAAMALPDAPIVDIDAQGSTWS
                     PDNYEGEFSGMVHLRRALAASINVISVRIYDIIGADTIIDFASRVLKVSPTRFHPGPS
                     LALGSSELTPFEMVTGYAIYANRGRDVIPFAIRYITDRDGNEIENIEEDIGNIIAAKE
                     ADGSIQIVPENVNNVMTSLLQSVAESGTPTEALRVVAKFDKKCAGKTGTTQNWTDAWF
                     CGFTPDIAAVVWVGYDKPFMSLGRHQAGAAVAAPIWGNYMREVYNGMPDPQWPAAPKD
                     VFYGATCKYSGCAPGPNCNELISDLMIPGGTPKCVCNGVHYKMKSVFERYMEKEGLKY
                     EE"
     gene            21095..21574
                     /locus_tag="PLV81_08850"
     CDS             21095..21574
                     /locus_tag="PLV81_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOM10242.1"
                     /translation="MFKVGDKIVYPLHGVGIIEAIEKKVVLSKRNEFYIITIINSGMK
                     VMIPTEKAESIGLRKVIAKKDVTKVLNLLKKSDVETEDDWKIRYQNNVDKIKSGSIFA
                     VAEVARDLYKRGKDKELSIMERKLYENAYQLMIHEIALAKDIDVDEAGNLVSEALSS"
     gene            21718..22719
                     /locus_tag="PLV81_08855"
     CDS             21718..22719
                     /locus_tag="PLV81_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HOM10243.1"
                     /translation="MIAIIRMLFIVTSALFSGLFFFQYSLQAAVIAAVIASVGALIFI
                     IAVEYISHTISTRMVIAALVGVFIGLIFAHLLVIAFVSIPLTIQPSTVKLISAIIYHV
                     VGYATVLFFVINCDDIAIFDTIVPVKREEGRDGASYKILDTSVIIDGRISDICDTGFI
                     EGILVIPNFVLNELQMIADSADSIKRNRGRRGLDILNKMQKDQRILVKISDVDFPEVN
                     EVDAKLVKLAKLMNAKIITNDFNLNKVAEFHGVKVLNINQLSNALKPIVLPGEEMAVS
                     LIKEGKDPNQAIGYLDDGTMVVVENGRSRLNSNVDVIVTSVLQTTAGRMIFARIKED"
     gene            22697..23281
                     /locus_tag="PLV81_08860"
     CDS             22697..23281
                     /locus_tag="PLV81_08860"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOM10244.1"
                     /translation="MPGSKKTDATIVALCGSPHIQGNTATALRLLFNELSGISKRIYF
                     AYKLAVQPCTGCGYCSRKFGCIFDDAMNELYDTLQRAHVIIIASPLYFSHLPSPLKSI
                     IDRCQVLWEYYNRGNKPLHRQHGFALLLGAGNYSNMFVPSVITLRHYFNSLNYLFDEK
                     KYLLLSDINNKKDIRNDDITKKITAAAHYIQKLL"
     gene            23294..24538
                     /locus_tag="PLV81_08865"
     CDS             23294..24538
                     /locus_tag="PLV81_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10245.1"
                     /translation="MKLYVTLTFSCTSEDLALFVKKVVESGCSIRSINYISSEGNYAT
                     YKADLMYTDRKIFKEKILSWVSKETTLIDCSDIMSKGIEGGLLKVSSKIPFDSAGEFE
                     GKILGYIEILKDNLITEESRKFTGMSSNCGMIILTEDSQQIQPREIYHYILIERDSLV
                     LSHCSTLNPQPLLMRYRTKEDILKIVDSVSTNYALMRIAMPDTFQEVSLFHQMDEIQS
                     LVLSREYDEIPLYCLIKIMNILKRHHVSLADSVIGIAGITTSSLRLTRLLYGIGCSKV
                     LGFDTSEVNLYHFEKEKGIATNIDNVFENADVVLFMSDCCTSEDFYKLGKGQIVLINP
                     AIHFDTLVFQERGLKDYELLITENSYLLLPGLAEKMIQGQIHSLNDEQLIAIGKSLYG
                     ANLDIDSAENMYSITETIRKVS"
     gene            complement(24544..25146)
                     /locus_tag="PLV81_08870"
     CDS             complement(24544..25146)
                     /locus_tag="PLV81_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10246.1"
                     /translation="MSNAITIIQKHELIANLTHLLNMDNGGRKYYLLEYEHISEMISN
                     VTEQFYDDLIIINSSYTILYSMKNIDLYGRRLSQLSPVLYYFVSNLPYDDIAIFKPFE
                     YPPMSGNYSFCVCYPIQLDDRNKGFCIGMINQNKFYDILPADSIIAETNGEVIFNIIQ
                     RGTLKLDENTLKKSSINVSGKKYNISLLKYKNITWYILYP"
BASE COUNT         8382 a         4021 c         5055 g         7941 t
ORIGIN      
        1 gctgatgtaa ttgtggcaca atcatcaaat acccgtaata atgcaataga atactatgca
       61 ccaaaaaaag aaattcagat tataccgttg gcattccatc ccccaataat accacaaaca
      121 tcacgaaaaa tattgggttt gcatgaaaag gattttatat gtataactat tggaagattg
      181 ataaagcgta aatcaattga tgtccttatc agggcgatag ctcttgttaa taagcctgcc
      241 gttaaacttc tcattatggg agatggacct gagagggaat acttagagga gctggttaat
      301 aaattgtcgt tacaggacag ggtaaaatta ttaggttttg ttggcgatga ggaaaaattt
      361 gcgtatctat cacaatctga tttgtttgta cttacatcaa tgcatgaagg atttggtatt
      421 gtgtttatgg aagccatgta ttgtggattg cccattgttg ccaccaatca tggtgggcag
      481 atagatttcc tttcccatga acaaaatgca ttgctcatta atgttggcga tacaaaagga
      541 tgtgctgaaa gtattatgag attttataac gataaaaagc tctatacacg ctgttctgtg
      601 aataataaaa aaaagatttc aaccttttat gccgaaaatg tagcaggtcg gtatatgaat
      661 atttttttaa gtctaataga tagaaaatga aaattaaaac agcactcatt acacaaaata
      721 aggaattggt tcaggaagta agaatgcttc agattttaga tgtaagtgtt catggtacta
      781 ttaatactct taaagatgag ggttttgata ttgcattatt tgatactgaa cagattgaca
      841 taagcgatgc acaattttat ctttcaaagt tacgtaagcg ttttcaacaa ttaccggtta
      901 ttcttattgt acgggcagga tatttagaat caataaacag ggactggttt tttgatgatt
      961 ttattgtgtt ccctttcaga aaaggtgaat taaaaacacg tattgaccgt ctaattggtt
     1021 ctgaaattga attaaaggaa tcggttatca aagttggcaa tattaccatt gagcccgaca
     1081 agtattcggt aacagtaaat aatgagaagg tagtgctgac atataaggaa tttgaattac
     1141 taaaattatt gatggaaaat aaaggtgtcg tttttacacg tcaggagtta ctttcccaga
     1201 tatggggtgt tgagtatata gggggatctc ggactgttga cgttcatatc aggagactta
     1261 gaattaaatt gggtgatgaa ttcaataata taatagaaac catacgaaat gtaggatata
     1321 agtgcaaaga ataattgtag aaatatgctt gccaatatat aaattactta attaatgtta
     1381 aaaaaatcta ttatttacga cgtagagagg attgagtgga actgttatga atcagttaaa
     1441 aaagattgaa aaggtgctgg aatcagcctt acatgaggat cttacaccat actttgctct
     1501 ggtaaaagca atgaggaaac atttaccgtt gtttggttca acaaaacgta taactgctgc
     1561 attactgaaa gaatgcattg gagatatctc aaagcttgat atagcggaag ttgcatcatt
     1621 aaactttaca aaaacttccg atggtacaat tatctttgaa gatattaagg atggtaaagc
     1681 ccgaatattt attaatattg gcaaaaacca caaaattacg ccaggagacc tcataaggga
     1741 aatatcaaag cgttcaggga ttgatgggaa gctggttggt aagattgata tacattctac
     1801 gtattcattt attgaaatac ctgaacaata tgcagaactg gtgttgttgt cacttgacaa
     1861 agcgcgaatc aaaggtgtac caattgtggt ggaaccggct aaaaagaaaa agaaacaatg
     1921 aaaagatgac accccactgt cctatctgta aatcatcaaa gtttaaaaga atttcagagg
     1981 atctgctact gtgtaagacg tgcagtatta tgtataatgc cagtcattca ccagcagtat
     2041 attctactga ttactttggc caggaatata aagcacagta tggcaaaacg tatgaagatg
     2101 attttgagtc aatatatgct gcatcacttt tcaggctttc aataataaaa aaatatttgg
     2161 ggaataacgt atctgcaaaa agccttcttg atattgggtg tgctttaggg tttttttgca
     2221 aagctgctag agatgtgggg tttggtgagg ttgaaggtct tgaaatatca gaatatgccg
     2281 taagttattg taaactcaaa tttaacatcc cggttcatat gacgggattt gaagatttta
     2341 ttcctgtaaa gaaatatgat gtgataaccg catggtttgt gctggaacat tttactgacc
     2401 cgtatgctat gttttataaa atatatggta tgcttgaaga taatggcatt tttgcctgta
     2461 cgttgccatc gtattttggg ccactatttt attgccacag aagtgagtgg atcagatcac
     2521 atccacagga tcaccgtata gatgtggcac catggagtgt taaaaaactt tttcagcaaa
     2581 ttggattttt tagcgtcaga tgttttccat cgggctacca tcctgaacgt ttaggaacta
     2641 tcgctcaaaa atataaaaaa ttgtataata ccattgcaaa cctgctttgc ttttctgata
     2701 ctatactggt gatagcatca aaaaagcccc aataaattct tgacacaaaa cacaatacgt
     2761 ttagaatagc atttttgttg tgtattacat aaaagttgtg ctattgtaaa aaatacatgt
     2821 aacgtagctg aaaattctat atatatttta ctgtaatcat atacgatttt tactactttg
     2881 aactaatatt cttaacaatg aggtgggatg atgaaaagga catttcaacc aagtgtaatc
     2941 aaacgattac ggacacatgg attcagggca agaatgagta ccagagcagg tcaggaagta
     3001 ttgcgcagga gaagacaaaa gggtagatac aaattaactg tttctgacga aaagcgaaat
     3061 tcaaagtaat tcttggtaat atcattattc cgtgaaaagg agatattcat taaaggggct
     3121 gaagagattt aaagaagtca ttgctaacgg aaaaagagta tatggcaaag ggatgagaat
     3181 gatagtgtat atgaaaccat tacaggcaga agggaacatg aacaatatat tacaatcccg
     3241 ttttgccata gtggttacta aaacctatgg aaatgcagtt gaacggaata gaataaaaag
     3301 gcaaattcgt tccattttgg ttactctgat gccccagatg cgacagggtt ttgctgttat
     3361 tatatttccg gacaccacct gtaaggctat gcaatataat catcttgttg tatcagttaa
     3421 acaagtattg cttaaatcag gagtactaaa acagtgaaga taaatatggt taccataccg
     3481 gtagtgttaa taaaattata taaacttgtg atttcaccgg tattacccaa tgcttgtaga
     3541 ttttatccaa cgtgttctca atatgcaatt gaggcattga ccaggcatgg acttttcaag
     3601 ggtggttttt tatcaattaa aaggatactt cgctgtcatc cattttgtga tggtgggtat
     3661 gaccctgtcc cctgattatc atttttaagt acggaaaaat attcccttcc tgttatctcc
     3721 tttccacggt atgatagtat acatatgtgg tattgttgta cctgagtaaa aaatttatca
     3781 agaggttgtc atggaaaaaa gggctttaat tgctgtgtta ttatcactgg ggatatggat
     3841 actgtggttt tatttatttc cgccacagga aaaaacacct ccggtcccgg aaaaaagtgc
     3901 tgaaactgca acagtgcaaa ctgaagaaat acaaaaacct gaaatttctg tggtttcaaa
     3961 atttcagcaa acttttacaa aaaaggaaga agttaccatt gatgcaaaat actatacagc
     4021 agtactttcc aatgaggatg gacaatgtac atcatttatt gataaagtaa gaaatataga
     4081 attaattgta actaaatcaa aatttgatgc aaaaggtttt tttgatttcc ctgttcattt
     4141 ttcaacaggt gagtttttgc atgcaaagga tatacctcct gcattatgga aaatgcaaaa
     4201 agatgcatca gcagtgcgct tttccacagt tgctatcgta caaaaaaatc cactggaaat
     4261 ttcaaagaaa tttacgtata acgaaacagg acaatatttt gttgtatcgt atgctatcac
     4321 taataaagga cagggtgaat ttacatttcc tgaagggggt gtaatatttt ccccatctga
     4381 ttttttaggg ccatcaatgg attttgataa ttcatataat gagttacatg gtatctatta
     4441 tattaatgat agttttgaaa aagcccgtaa gggtggcgga tttttttcag caccagaaga
     4501 cctcaagagg gaaaacggtg tggtacaatg ggctggcata atgagcaggt attatctgtt
     4561 aattatgata ccacagggtt ttgctggctc aggtgtagta tacgataaca ggaagcatac
     4621 gggtttcagg acgggtattg tggtaccggt caaaccactg aaacctggtg aaacaataac
     4681 acatgaattt aaagtatatg ttggtccaaa aaataaagat aagcttcttg ctgttgacaa
     4741 gcacattggt gatgctgcgg atgtcagcaa gtggattgaa cctatcaggg actttatgat
     4801 gtggtgcctg cttgaaataa acaggctgat aggaaattta gggtggtcac tggtaatatt
     4861 ttctgttatt acaaaaataa tgtttttacc attgactcaa cgctccacgg aatccatgaa
     4921 gaagatgcag gaattgaatc ctgtgattca ggaattgcgt gaaaaatata aggataagcc
     4981 tgatgttttg aataaaaaga taatggaagt ttacaaaaag aataaagtga atccaatggg
     5041 cggttgtttg ccgctgcttt tgcagatgcc tttctttttt gcactgtaca gcgctctgat
     5101 aaattcagta gatttatggc aggcgccctt tattttgtgg ataaaggatt tgtcattgcc
     5161 tgatacaatt tacaggatag ctggatttaa tattaatatt ttgcccctgg ttatgacagg
     5221 aacaacatat ctgcagcaaa agatgtcttc atccgaagtc actcagcaac agaagatgtt
     5281 aatgctcatg ccgttaatat ttattgttat cttctggaat atgccctcgg ggctggtatt
     5341 gtactggatt atgcaaaatg tattgcagat aggccatcag atgcttatta ataaaattgg
     5401 caaaaaaaag taatacaaat ttttgtgtga aatgtgagga ttttataagt acattatctt
     5461 caggaaagtt acccatagtc gttacaacca ccatgcgaag tggttaatat attaatgagg
     5521 ttatgcagat aagcgatttt gttagatcat tttgatcaac atgttataca gtatttgaga
     5581 ttaaagtact aaggtgaaat atttataatt aaaaggaggg aagatacatc accatacaaa
     5641 caactgaact attattatca aagtttcgta ggaggcgtga acatgaaggt gttagatatt
     5701 gaagggaaaa ctgttgaaga agcaacgaag aaagccttat cattattaaa aattaatgat
     5761 atgaacaaaa tcaatattga agtgctggat gaagggaaaa gcgggatctt tggttttggc
     5821 atatcacgcc ctgctaaaat acgagtatat taccaacagg aagatgatat tggccaacag
     5881 gcaaaggaaa ttattgaaaa gattttgatg ctgatggaag ttgacgcaaa ggttaaagat
     5941 tataaggaaa gcgaaaataa agtatatgtt gaactggaaa gctcaagttc gggattaatt
     6001 attggtaaaa aaggtaaaac cctggaggca ttgcagttta tggttaacct tctggtcaat
     6061 aaaatgacca attcagaaaa aaagatcata cttgatattg aatcatacag ggcaaaaaga
     6121 gaaaaagcgt tgcgtaaatt atccaaagaa attgcattga aagttgcccg aacgggtaag
     6181 ccatggacgt tagaacccat gaatccattt gaacggcggc ttatacattt gacattgcaa
     6241 aatgattcaa aggttaccac caagagtgag ggacagggca tttacagaaa ggtaaaaatt
     6301 atgcctgtgg aaaaaaggta atgcagatgg atgatacgat atgtgctccg gcaactccgc
     6361 cggtacattc accaatagcc attatacgga taagcggccc tgattcatta cgggtcgctt
     6421 cttctatatt cagggcacaa cagcatacac aggaatttat tccgcgatat gcgtattatg
     6481 gtatcattca tgataacaat gaagaaattg atgattgtat tgtaatctat tataaacacc
     6541 cgcaaagcta cacaggtgag gacatggtgg aaatattctg tcatggcaat ccgatcatag
     6601 taaataaaat aattaattgt attgttgcac tcaatgtgcg gatagctgaa ccaggtgagt
     6661 ttaccaaacg tgcattttta catggcaaga tggacctgac cgaagctgag gctataaacc
     6721 atattatcgc tgcacgcagt gaatgggaga taacaactgc aatacagcaa aagcatggat
     6781 cactgaaaaa tgcaatcaat gatattaaac agcacatcat tgaattaaaa gcagatattg
     6841 aatgtgctat tgactttgtg gatgaggata ttgaaattat atcgtttgaa aatgcacagc
     6901 aaaggatgca cacgataatt aataaaatac aggacattct taatagatgc aggattggcc
     6961 agcacctttc tcacggcatt gatattgcaa ttgttgggaa gcccaacgca ggtaaatcca
     7021 gctttttgaa ctgtatactc aatcatgaac gagcaattgt gtcagatacg ccagggacaa
     7081 cccgcgatgt gataaaagaa tcggtttcaa ttaaaggtat tcaatgcaac ttgcttgata
     7141 cagcgggtat acataaaaaa gcagatggta ttgaactttt aggtgtgcag cgcagctaca
     7201 ggctgataga tgaggtgtct cttataattt ttatcattga tgctgtgaca ggcatgactt
     7261 ctgacgatga acacatcctg gaattgatta aggaaaaacc gtttatacca gtcattaata
     7321 aaactgatat tgcaccaaaa gaatcaatag caataattca ggatgccatt ggtcaaaaag
     7381 gaattgtctt ttctgcaaaa acaggctatg gtattcctgc acttgaggat gcagtatatc
     7441 aatgtatcag gtcacagtat gttgaataca aaaattcatt tattgccgac attcgtatga
     7501 ttcagttatt ggaacagtct ctggtatatg cacaggaagc gcttcaccag atggctatca
     7561 gcagcccaca tgaaattgtt gcatcagcgc ttcaaaatgt tatcgataca ataggagcgg
     7621 tcactggcga aatatccgtt gatgatgtcc tgaattcaat tttttcacgc ttttgtattg
     7681 gcaagtgatg ttacagcatt atgaataaat tattaataat aacgaccatt atactggtgg
     7741 cgatagttcc tgtaaaagcc ctgcctgatg tcagtgaaga cagtaaacga atatactgta
     7801 acggcaattc actcagtata atagaacagt atgaaaaaga aatagtatca gggaaagcgt
     7861 atcccgcatt aattctggca gatattcatc atatacttca agttgcaggt atcaacaaaa
     7921 cattaatgtt gctcaaagaa ttaagcagaa gtgctgatac cgatgctgaa agactttatg
     7981 ttcaggatat tgttaatgta attgaagagt taaatcaaaa agacatcaca aaggatatca
     8041 atcgatggga tatatacgat aatatcaaag aaagaatgcc aagaagcata acatcccttc
     8101 aatgcacaaa taacgcttgt tttttatccg gtaaaaataa gatacgagta tctcaatatc
     8161 atagtggatt tatccctgtt aagcaattta cccaggatta taacaccatt ggcctttgca
     8221 gtgggtcttt taaagccact gtacctgtta cactcaggat ttattcaaat atggattatg
     8281 tatgttatat taacggtaaa aaaatatgtg taaatgctct gacagataag aaaaagctgt
     8341 tgcgaatgtt tcatatagaa tcggaagaag gttttactgt tgcactatat tataaaccag
     8401 tagaaaacat gttcttgaaa atacagtttt atgatgatac caaccagatt atacatttac
     8461 ctgatacaga aaacacattt tatcatgatg tccagtggta tgaatcatat tatgaatatg
     8521 aaaatcaatt acttacacaa tataacaatc aacgttctgg cgaagcagct ttccagttag
     8581 cactgtttta tgaatcactg caaagcatag aagcattaaa atattataaa gaagctctcc
     8641 aatcaaaaaa tgagtttata gcctgcagat tcttaggttt tttgctggaa catgaaaaca
     8701 aggttccggg atctgatgta gtagtgcaaa ctgtacttaa aaaaaataat gaaaaatata
     8761 catcagtatt atggcaaaat tactatgatt atatttatca acggaaatct ttagatcctt
     8821 ttcaggtgaa ttatctacct ttactgactc atctgctgca tcgagaaaaa gatgtactga
     8881 ttaaaaaagg tgtcaatctt gaacctttac ttgaacagta tcctttcagt aacgatctta
     8941 gctatatagt tgctaaaatt actgcttacc atgacattac aaaagctatt agtgtattag
     9001 aatcaataca agaaccagat gacaaagaga tagatttatt gatacaatgg tatgaacaga
     9061 atgagcagca cgaatcgata gtatctattg ttgagaacaa taatatagat agatattttg
     9121 ataattatat acatgccctc atagcattaa aaaggtacaa tgatgcaaaa agtgccttgt
     9181 ttaaaagaat agctcagggt tttgattcac atgcatatgc cttactggca acaatagctg
     9241 accttgaaga ttctgatggg agtatgtacc ggcagaagca tgaggtgata gtttccggca
     9301 taccatggca tagtgattat atgaagagtg tatttgatga ttatgtgaat aaaactgttt
     9361 tttcacgatt ggcctttgaa actatccaca gaaaaacgtt tgggctattg tattcctgct
     9421 atgcattaag aattattgat aacactgcat actgttctct ttatgacctg tattatgcga
     9481 atagcctgtc acagataaat gattatactt tttctaaaga tgtaacaata acaggatgta
     9541 caatatatct ggtcagtgaa aacggaaaga ttgaaaaaaa agaaataaaa aatatagaca
     9601 attcatttca aaagggcatt aaggcatgtt ttgacgaaaa ccagaatacc tatgttctgg
     9661 tagaattatc atttatgata cacaagaaat taccggtaat acacatccag cagaattaca
     9721 atctatcaca atttgatatg gacattattg ttattgatcc acagggcaat cctacaatta
     9781 catcagtaat gaagggcaat attacgaaag gcgagaatgg catacagcac atcagatttt
     9841 atgctgatga gctatcgaca catcataaca ggaattttat ggtgatagca cttacacctg
     9901 aaaacctgat tgaatggatt aatgaacaga gtgtacgttt caaaattgca gattattttg
     9961 ttactgatac tgaattaaac ggcaaggcag ttgaagacaa ggtagcagag cttatgggaa
    10021 aattgaaaca gtataccata catcccaacc cttatacaag cacatcgttt ttacagtttg
    10081 tgggtttagg gaaaggcaca caacttgatg caatactgta ttcacgctat atgctggcaa
    10141 aaaatggaat catgtcatat attgcgcttg gttgttcaag ttcaaacaaa tttactgaaa
    10201 agctcaataa ggataatggg ttattctttg atacagtgct tctgtatata ccgctttcca
    10261 ctgaaaaagg gatatggttt acctgtgatg gcgcatatcc ctcttctgat aaaattaata
    10321 ttaataatat tctacttgtt gttggcgatg agattattct aaaatctaaa aataattgat
    10381 actacttagc taactttttt gccagtaatt ttttctttct tttgagtctt cgttttgcgc
    10441 gtttacgctt aatgcgtgga ttatactgct tgctcataaa ttatttatcc tccctgaagt
    10501 tattacataa cacagaaaaa atgatatcag ctaatgtcaa ctgatttttt atattccccg
    10561 gatttcacaa aaggtagcaa atgtttttac aaactgctgg aatgagtata atatatcttt
    10621 ggggtaaggt ttaactgtat gaagtgattc gtcaagtgtg tgctggttat gaaattgctg
    10681 aagataataa tattttacag tacccacacg ctcttttata ctttgaagat cttcaatagt
    10741 aacaaatcct ggtacgcagg ttgtccttag ttcaaaagtt atatggtgtt ctttgagtag
    10801 tgcaatagat tccataatta aatcaaatgg cagttttgtg tttgtcaata ctggatattt
    10861 ttctggtgat gttttaatat ctattgccac gtagtcaata agctgctgcg atagtaattt
    10921 tttcaacact ttaggctgaa atccattagt atccagttta acttttaagc ctgtttcttt
    10981 aaccttttct aaaaaggtaa ctataccctt agcaagtgtt ggttctccac cagtaatgac
    11041 aacaccacca ataaggtgct ttcgtttttg gagaaattcc agcacctcat ggtttgaata
    11101 ctgttgtaaa ttacaatcat tgcacgccag ggatgcatta tggcagtaaa gacagcgtag
    11161 attgcatcca ccggtaaaaa ggacagtgct gataacctgc ggaaagtcaa tgagtgaggt
    11221 tttctgaata ccccgaatat tcatgtgtgg ctaccttaaa taattcatct ttcataaact
    11281 cttttctttc atgaaattct tcctgtttac ctttattcca ctggttaact ggcctgaaat
    11341 acccaacaac ccttgaatac acttcaacag gtatcttttt ttcgtgatgc ttttgcatta
    11401 cataaccccc atttaaaata gtaacaaaaa tatatattcc ttactgttgt atctgtgctt
    11461 cgattgacaa tggcttacta tcttcaattt cgacatctat gccaaacatc tcaagttctt
    11521 cttttgtatg ttcgtatgga cagaatttat gattgcccgg tatataccca tgaacagggc
    11581 aaatgctgaa tgttggtgta atggtaaaat acggtatatg atagttgtaa gcaatagtct
    11641 taaccagaag ttttaccatc tgcggatcat caatcttttc gcctataaag caatgaacta
    11701 ctgtaccccc ggtaaatttt gactgcaact ggtcctgatg atccagtaaa tcaaatatat
    11761 catgagcaaa tcctactgga ggatgaattg agttggtgta atatggttcg tttttgccgg
    11821 atgttatgat gtcaggaaat ctattcttat catggcgggc aaaccggtaa cttactcctt
    11881 cggcaggggt agcttccaga ttatataaat tgcccgtttc atgctgatag gtggtaattt
    11941 tatcccgcat aaagtccagc acctgagcag taaactcttt accttcttca gttgttatat
    12001 cctgacctaa aagattgatg caggcttcgt tcataccaac aaggccaatc gttgagaaat
    12061 gattaaccca gtatttttta aatcgtttat atacatctcg caaatagaac tttgtataag
    12121 gataaagatt attggcagta aagttttcaa gcactttacg tttaatttcc aatgaatttc
    12181 gagccacatc cattaaatgg cccaaactat caaaaaattc atctttggtt ttggcaagat
    12241 atccaagacg cggcatgttg atagttacca caccaatact tcccgttagc gggtttgcac
    12301 caaaaagtcc gccaccacgc ttgcgcaatt ctctgttgtc aagtctaaga cggcagcaca
    12361 tggaacgtgc atcatccgga tccatatccg aatttacaaa gtttgcaaaa tagggtatgc
    12421 catattttgc agtaatttcc catagcaggt ttattgcagg attatcccag tcaaaatctt
    12481 tagttatatt gtatgtgggt atggggaatg taaaaattcg atggcgggca tcgccttcaa
    12541 gcatgcattc agcaaatgcg gtattgaaaa tatccatctc tttctgaaac tgtgcatatg
    12601 tttcgttcat gaattgtcca ccaatgatca catgttcatc agctatagag tggggaaccg
    12661 taagatcaag tgtgacattg gtaaacggtg tctggaatcc tacacgggtg ggtatattca
    12721 tgttaaaaat aaattcctgc aagcactgtt ttacgcctgc ataatccagg ttatcgtagc
    12781 gtatgaatgg agccagataa gtatcaaagt ttgcaaaggc ctgtgcaccg gctgattcac
    12841 cctgcagggt atagaaaaaa ttaactatct gtcccaatgc acttctgaaa tgttttgcag
    12901 gtctgctttc aactttgccg gggacaccac caaaaccggt tagtaaaagg tcacgtaaat
    12961 cccagccaac gcagtatgct gaaagcagcc ctaaatcatg taagtgcaga tcgcagttaa
    13021 catgtgcatc tctgatttcg ggagggtaaa ttcgttcaag ccagtattta gcggttactg
    13081 cagaagcaat gtgattattt aaaccctgca acgaatagct catattgctg ttttcattaa
    13141 cacgccagtc tgacctgtca aggtaatctt caataagctg aataccttca tttaaaaggc
    13201 ttttcccttc acgtattttg cggtgctgct cacggtacag aatataggct tttgcaactt
    13261 ttgcatgacc tttttcaatg agcatcttct caactaaatc ctgaacattt tcaacagtgg
    13321 gaatgtgctc acctttatat aaaagtgaca atatatcaac aaccgattca gcaatttgag
    13381 ctgcagtatt tctatcagtg ccaccaacag cgcgggcagc cttgaatatt gcttctgtaa
    13441 tcttttctat attaaaagga actatcctac catcccgttt tcttataaac tgggggctat
    13501 cttttgaatc tgtcatataa aaactccgta aacggtgaat tagtatgaat ggtgaaaaaa
    13561 actataatgc aatggttaag gattgcttgc tacgggtatt tttaaaaaac agtgacatac
    13621 tgaaacaaaa aagtcaataa ttttatgtca cataataaaa ataatttgct atacatatat
    13681 tagtataaat atgtatgtat attctttagt caagaaaaaa ttaaatggta aaattttatt
    13741 atttttccga tagtatagtg atattaatca gtaaaatata aaaggtaaag ctatgagaat
    13801 aaataatgta gcgatagtta tatatttaag tgttattatg gctttgggca tggctatggg
    13861 gatggtatat tcagatgata attcactttt ctcaactctt gcaataactc ctgaagatgt
    13921 gcctcaaggc tttatatttg ggaaaatacc taattttgca aagaaggtgt tcactggaaa
    13981 tccatgtatt gtgaattcag atggaatacg atttttagct tccaaattat atccggaagg
    14041 taatgctgca aatatcaagg gtatgtatgt tgcaatcatg gcttctgaat caaggccgta
    14101 cggagatgat ctggtgtact atctgcttgt ttttaaagat agtaaagcag cttcagttga
    14161 aataaaaaaa cttcaggatt attatcagta caacaaagac agaatagtcc tcattacaaa
    14221 agacaacatt gcgctattgc tctttgctga tgatgtagat aattataaat atattgcaga
    14281 gttgggacag aaactacagg aaagactcaa ctaataaata ttttttgtga tgagcatctc
    14341 ttctaattca tctgcttttg ctgcaatata aaatagtgat ttcataaatc gctgtggatc
    14401 acaatctttt agcaaaagag aattccttaa aattaatgtg tcattgagca atgccagggc
    14461 gccataatca agtgtactgt tgagcttcag cgcaaactta tacagctcaa atgattcatt
    14521 ttttatagtt cctataactg aatagtaatt tacaatcctg tctcctgact catctttgtt
    14581 cagagtaatt aaaatttcct gagatcggtc attctcaaat tcaatggttg tggtatatgc
    14641 accctctttt gttttatgaa attttaactg aaattcggaa gcaattgctg caataaatcc
    14701 atcaaataat tccatacatc accttttact gtaataattt tttaggccaa tagtttctga
    14761 atatgctcat actgtaatgg tttatgggca taaaaaacca tgttctgatt ttgttctata
    14821 gcacttttta ttttattaat gtctggcgta tctgtacctg caggatggct taaagccatg
    14881 tacatttgct tttgtgtatt gtcaagaatt tgtgcatgtg aggtgagtgt ttcatgtatt
    14941 gctttcattg atgctgtatc attatttttt gctgcgtttc ccaattgctc aataagcttt
    15001 gttgtttcct gcaatgtgtc agattgagga atatttggaa cagaagctga acctggtgaa
    15061 aggcgagtaa aataattatt tatagttgca attttagcaa ggatttcatt gtggtcctga
    15121 ggtgtaaatg ctttctgtat aagttcatag gatatgttta tagccatggt aacaagctga
    15181 tgggcttcgc tgataatggt ataggccgaa atagcttttt tcagtgaagg ataaacccgt
    15241 acagattcca ctggttgctg tatttgaata cttgcttttc tatgagtagc cagtgatgct
    15301 tgtggtacta ctggtatggt atttgttatt ctcatagctg cccccgacca gataaaaata
    15361 tatagtatgt atacaataat gtcaacaagt atttattgtg gatgttttgg acatatagga
    15421 attactactt tattttaggt agccgtatat tctgctgtag atacttcaat ggatgaaaat
    15481 aacacaagaa tggcatggtg acaatagcat gagcgctctg gatggggtat cttagcctgc
    15541 tggagtgtgg ccatctctgt taaggaaaga aagtgcttct ttaatttact ttgggatagt
    15601 aatgacttga tatgctaaat ttaatttatt aataaaatgg ataaaaaaca atattgaaaa
    15661 ttaggtaatg gtattgtact gttgtagtaa ttatgttgta cgtgattatt gattaaaaag
    15721 gagttttcaa tcatactaca tccttgccgt atttggtaaa gggataaata ttatggtaga
    15781 gtttttatga aaaaattagt cattatactc tgttgtatat gtatgataag tagcatttca
    15841 tgcaaaaaga aagaagaaaa cagaataccc cgtgagcaaa ttcagattga tggggcacat
    15901 gattattctt taatatttaa agatatcata gataaagaaa aaaaggaaca acaacggtat
    15961 aaaaaaggtc cctttgatga ataagctcat tgaaatccct aaagagagtt taattgccta
    16021 ttgtctgaat ataccacggc cagtaatgga atcagttatc ataagggaaa tcgtgaatcc
    16081 tttatgcaat ggtcattcta cattgtatca aaagcatttt tcgctgtttc atgcattata
    16141 caatataaaa aataaccctg attactttaa ctatatggta catattcatc caatgcaaat
    16201 agttatcagg aactatcctg taaataataa atgcattgac tataatccct tgacagatag
    16261 tttttgtgga aatcaaaccg tggatggaac atactgtcca tttcactata cttttcatgc
    16321 accctttaga ggggatatga tatatgatcc gctcaatgag ttttacagtg acccgtacaa
    16381 tattaattac gaattttcgg atgaattgaa aaaagtatat agtggcttta caaaatatat
    16441 tattaatcgc aggaaggttg agcaggctct atcatttttt tcatttagcg ctatggagca
    16501 ttgtaataag gcattactac aaaaacgtta tcgtgaactc gtgaagaaat atcatcctga
    16561 tcattaccaa ggtgatacaa caatgattaa ggaaattaat gcacactata cccttttaaa
    16621 ggaaattttt gtagtataat actatatgaa gtggcattca taaatatgaa tttttttgta
    16681 gcatttttac cagttaacat actaatctgg tcatgaaagg tatttactaa aactattgta
    16741 tcaaaataag gtaaataata tattataagc gatagttacc ataaaataaa aggagaatga
    16801 tatgaaaaga agagcattcc agattattcc agtgatagtc ttgataagcg tcgtgctttt
    16861 tgtgtttaat aatttttcct gttccaatag cggcccatct gcgtttggca atagcagcag
    16921 ttcacccctg aaaggggatc aggaaacagc acgggctatt gagattcagg atacattcag
    16981 aaaaatatat gaattataca aggacagagt ggtatttatt actactgaac aggttgttga
    17041 gttgccgcca aatccattct ttgatgatcc ttttttcagg cagttttttg gtgtacccga
    17101 gcagaaatca cgcaagcaaa aacgaagtgg tttgggcagt ggatttgtgc tatccagtga
    17161 tggatatatt tgcacaaatt atcatgtaat agccaatgtt gactcagtta aggttaaagt
    17221 gaaaaataaa gactataaag ctacagtagt tggttatgac cagcgtaccg atttagcatt
    17281 gctgaaaatt aatcctgatg aaaaactgga acctgtattt ttaggagatt ctgatcaggt
    17341 aaaggttggt gattgggcta ttgctatagg gaacccattt ggccttgaca gaacatttac
    17401 ggttggagtc ataagcgcaa caagccgcaa agatgttgat atgatggggg gttcccagtc
    17461 acatattcaa acagatgcag caattaatcc gggaaattcc ggtggaccac tggtaaacat
    17521 taatggtgag gttattggaa ttaacaggat gatctattcg caaagtggtg gttacatggg
    17581 aatcggtttt gctatcccca ttaatacagc aaaagcagtg cttgaacagc ttaaaaaata
    17641 caagaaagta aaaacggggt acataggtgt acagattgta cctttaacag aagagtacgc
    17701 taatgaatta ggacttaaga atacagaagg tgcactggtt ggtggtatta tggaagacag
    17761 ccctgcagaa aaggcaggga tccagccagg tgatgtaata ttatcagttg atgaaaaacc
    17821 cattaaaacc tatgaagatc tggtggaaat cgtaagcaaa acaccaattg gcaaaacctt
    17881 aaaaattggt gtctggcgaa ataaatctaa aatatatctg tttgtaactg ttaaggaaag
    17941 accataaatg ttttatagtg tatgcaatta acaataagtg atagataact gtatgcatca
    18001 tggataataa atatcgttac atagtaataa tagcagggat agtagcgata gtatatatag
    18061 tatttcgcta tattatcccg ctattattta aattattggg tataattgta agttttgttg
    18121 cttacagtat tgctatcgtg atagctatag caattgtaat acttatcatt ggctatgtag
    18181 cacgtatcta taaaactcat actatgtaaa aatagttgac gtatatagaa taataagagg
    18241 gatatcatgc atattcctct ttgctaagta aacaattagt gatgatttca aagcagtgag
    18301 gaaaccttac aaaatactat agtgatagta ggggctgtac caaaagaggt cgttgagtaa
    18361 attttgcaaa cattatattg taatactcgc tcatttcgat acaattttgc cacaaaatct
    18421 ggcaaaatta ctcaatgagc gttacgaaat ttgtatcgaa actacttttg ggacagcccc
    18481 cttgatgtaa caatagatta cattttttga atgatatgca tgaaatcatg ttttagacgg
    18541 agtatatagt gtgaaagaac aaataaaaat ctggctggat aaaactaaga acatcatcaa
    18601 tgtatatatc aacgatgtgc gtacatggtt ttatggtatt attgcggtat caatcattgg
    18661 aggactgcta tttggatata ttgtagctca ggtgcagaat tgtctgggaa tagacaacct
    18721 gaaacggttt cagccaagtg ttcctacaaa actgtatgat gttaatgggg aactcattgc
    18781 cgagcttttc caggaaaaaa gagatctggt gtcttttgat gaacttccaa aaacgcttat
    18841 caaggcattt gtagcaactg aagaccagga cttttatgca cacattggca taagtcctat
    18901 ggctatggct cgggcaatgg tgaaaaatat tatctccttc aagattgttc agggtggttc
    18961 taccattaca cagcagcttg caaaacggct ttttacttct ggagaacgaa cattcacaag
    19021 aaaagcactg gagcttgtat taacactgca aatagaaaaa cgtttcacca aagatgaaat
    19081 tttggagatg tactttaacc agatatattt agggcatggc tgttatggca ttgcatctgc
    19141 agcagaatta ttttttaata aagaagttag gtttttgagc ctggcggaag gtagtgtgtt
    19201 agctgcgttg ccttcagcac ctggaaggta ttcacctttt atgaatacac gcaatgccta
    19261 ttataaaaac tgggatattt taaacaggat ggttgattgt ggctttttaa caaagcagaa
    19321 agcagaaaca gtctatacct ctttctggcc ccaatatgta gatgcaatta aaacagaatt
    19381 tcctacaaaa acagcgtatt ccagaataat tgataatgca ccttttttca ctgattatgt
    19441 ccgacagatt ttgatttccc ggtatggcaa ggatgtggta tataatgaag gacttcaggt
    19501 gtataccaca ctgaatttga agcgtcagcg tgcaggagaa aaatatttaa cagaaggctt
    19561 gataaaacaa aatgaggtat cagcacgggc aaaccagtac atgtcaggag ctgttgacag
    19621 gggtttattt tctgtatacc agcagttggg catgcttttt aaccttccaa gagttatagt
    19681 aaaaagtgat accgaaacta tatttaaaaa attcatggta gataacctcc ttgatgaaat
    19741 ggagcttgta tcgttattat gcaatgccga tgccatgcat aatgatatag aaaaatttca
    19801 tgtagtgacc agtggcattt caacttcttt aaaggttgaa ggtgctttaa ttgcagtgga
    19861 accaaagact ggctatatca ccacaatgat tggtggttct gaatttgcag taagcaatca
    19921 gtataacagg gcaattcaag cacgacgcca gcctggttca gcgttcaagc cttttgtgta
    19981 tggagctggt attgaatcta aactcattaa tgctgccatg gcattgcccg atgcaccaat
    20041 tgtggatatt gatgcacagg gaagcacctg gagccctgat aattatgaag gtgagttttc
    20101 cggtatggtg catttgcgga gagctcttgc tgcatcaatt aatgttatct ctgtgcgtat
    20161 ttatgatatt attggtgctg atactatcat agattttgct tcaagagtgt taaaggtttc
    20221 cccaacacga tttcatccag ggccttcact ggcgttgggt agttcggaac tgactccctt
    20281 tgaaatggtg accggatatg caatatatgc aaaccgggga agggatgtta taccgtttgc
    20341 aatacggtat attactgata gagatggcaa tgagatagaa aatattgaag aggatattgg
    20401 caacatcatt gctgctaaag aagcagatgg atcaatacag atagtgcctg aaaatgttaa
    20461 caatgtcatg acttcactgc tgcaaagcgt tgctgaaagc ggtactccta ctgaagcact
    20521 gcgcgttgtg gcaaaatttg ataaaaaatg tgccgggaaa actggaacaa cacaaaactg
    20581 gacagatgca tggttttgtg gatttacgcc tgatattgcc gcagtagtat gggtggggta
    20641 tgataaacct tttatgtcac tgggaaggca tcaggcaggt gcagctgtag cagcgccaat
    20701 atggggcaat tacatgcgtg aagtatataa tggcatgccc gatccacagt ggcctgctgc
    20761 acctaaggat gttttttatg gggcaacctg taaatattca ggatgtgcac cgggtccaaa
    20821 ttgtaatgag ttaatcagcg atttgatgat accaggtggg actccaaagt gtgtatgcaa
    20881 tggtgttcat tataagatga aatcagtttt tgaacggtat atggaaaaag agggcctgaa
    20941 atacgaagaa tagtatttta aaaaaaggca acaatgctcc tgcctatcgc ataaaaataa
    21001 aatattttat tcttgatttt ataatttttg tgtgctacta ttaatagctt gtaggttata
    21061 ttattgcggg ttaatttata tatgagggta tcctatgttt aaagtgggtg ataagattgt
    21121 ttatcctttg catggggttg gcattataga ggctatcgaa aaaaaagtag tattaagtaa
    21181 acggaatgaa ttttacatta taaccattat taatagtggg atgaaggtaa tgattcccac
    21241 tgagaaagct gaatcaatag ggttacgcaa ggttattgcc aaaaaagatg taactaaagt
    21301 attgaacctg ctgaagaaat cagatgttga aaccgaagat gactggaaga taaggtatca
    21361 gaataatgtg gataagatta aaagtgggtc aatttttgca gtagccgaag ttgcgcggga
    21421 cctgtacaag cgggggaagg ataaggagct ttctataatg gaacgaaaac tgtatgaaaa
    21481 tgcttaccag cttatgatac atgagatagc cctggcaaag gatatcgatg ttgatgaggc
    21541 tggcaatctg gtatccgaag cgttatcctc ataaactacc tgcagtacta taacctgtat
    21601 tctcgcaaat atttacgttt tattgtatcc tgcatcacta cattgttatt atattattat
    21661 taatgtaata ggatgggtat aatattattt ttaaaaaaat aaaggagttc aaaggctatg
    21721 atagctatta tacggatgtt attcattgtc acctcggcac tattttcagg cttgtttttt
    21781 ttccagtatt cactgcaggc agcagttatt gctgcagtta ttgcttcggt gggagcactg
    21841 atatttatta ttgctgttga gtatatttca cacaccatct ccacacgtat ggttattgct
    21901 gcgctggttg gagttttcat tgggcttata tttgcccacc ttctggtaat agcatttgtt
    21961 tcaattccac ttactataca gccgtcaaca gttaagctta tcagtgctat catataccat
    22021 gtagtggggt atgctactgt tttattcttt gttattaatt gtgatgatat tgcaattttt
    22081 gatacgatag ttccggtgaa acgggaagaa ggacgtgatg gggcttcata taaaatactt
    22141 gataccagcg ttattataga tggcaggatt tctgatatat gtgatacggg gtttatagag
    22201 ggtatactgg ttattcctaa ctttgtgtta aatgaattgc agatgatagc agattcagct
    22261 gattctatca agcgtaacag aggaaggaga ggcctggata ttttaaataa gatgcaaaag
    22321 gaccagcgta tccttgtgaa gatatccgat gtggatttcc cggaagtcaa tgaagttgat
    22381 gccaaattgg tgaaattagc caagctaatg aatgcaaaaa tcattaccaa tgattttaat
    22441 cttaataagg ttgctgaatt tcacggagta aaggtactga atataaatca gctttctaat
    22501 gcactcaaac ccatagtatt gcctggtgaa gaaatggcag tatcccttat caaagaaggc
    22561 aaagatccca atcaggcgat tggatactta gatgatggaa caatggttgt cgttgaaaat
    22621 ggaagatcaa ggctcaattc caatgttgat gtcattgtaa catcggtttt acagacaact
    22681 gcagggagaa tgatatttgc caggatcaaa gaagactgat gcaacaatag ttgcgttgtg
    22741 tggaagcccc catatacagg gcaatactgc tactgcactg cggttattgt ttaatgaact
    22801 atcgggtata tcaaaaagaa tatattttgc ctataagctg gctgttcagc cctgcactgg
    22861 gtgtggatac tgtagcagga aatttggatg tatttttgac gatgcaatga atgaactata
    22921 tgataccctg caacgtgctc atgtaatcat cattgcttca ccgttatatt tttcacacct
    22981 tccttcaccg ttgaaatcca ttattgatcg ctgtcaggtt ttgtgggaat attataatcg
    23041 gggtaataaa cctctccacc gacaacatgg ctttgcattg ttgcttggcg cagggaacta
    23101 tagcaatatg ttcgtgccat cggtgattac attacgacat tattttaatt cacttaacta
    23161 tctctttgat gaaaaaaagt atttgctgtt gtctgatata aataacaaaa aagatataag
    23221 aaatgacgat attactaaaa agattactgc tgctgctcac tatattcaaa aattgttata
    23281 aaaagaggga agtatgaaat tatatgttac actgaccttt tcctgcacaa gcgaggatct
    23341 ggcattgttt gttaaaaagg ttgttgaatc aggatgttca atccgcagta ttaattatat
    23401 atcctcagaa ggaaattatg ctacctataa agctgatctt atgtatacag atagaaaaat
    23461 ttttaaggaa aaaattttaa gctgggtatc taaggaaacc acattgattg actgcagtga
    23521 tattatgtca aaaggtattg aaggcggttt gctcaaggta tccagcaaga taccgtttga
    23581 ttcagctggt gaatttgagg gtaaaattct tggttatatt gaaatactta aagataatct
    23641 aattacagaa gaatcacgca aatttacagg gatgtccagc aattgtggta tgattattct
    23701 gacagaagat agtcaacaga tacagccaag ggaaatatat cattatattc tgatagaaag
    23761 ggactcactg gtattgagtc attgcagcac actgaatcct cagccacttt taatgcgata
    23821 caggacaaaa gaggatatac tgaaaattgt agattccgtt tccactaatt atgctttgat
    23881 gcgaattgcc atgcctgata catttcagga agtttcactt tttcaccaga tggatgaaat
    23941 tcagagtctg gttttaagca gggaatatga tgagattccc ctttattgcc tcattaagat
    24001 tatgaatatt ttaaaacgcc atcatgtctc actggctgat tcagtaattg ggattgctgg
    24061 gattacaacc tcatccctgc gtttaactcg actgttatat gggattggat gcagcaaagt
    24121 actggggttt gatacaagtg aagtaaatct ttaccatttt gaaaaagaaa aaggcattgc
    24181 gacaaacatt gataatgttt ttgaaaatgc ggatgttgta ttatttatgt ctgattgttg
    24241 taccagtgaa gatttttata agttggggaa aggccagatt gtgttaatta atccagctat
    24301 acattttgat acgctggtat ttcaggaaag aggtttaaaa gattatgaat tactcattac
    24361 tgaaaattct tatctgctgc tacctggcct ggctgaaaaa atgatacagg gacagataca
    24421 ttccctaaac gatgagcaat tgatagccat aggtaaatca ttatatggag caaatcttga
    24481 tattgacagt gctgaaaata tgtacagtat tacagaaacc atacgaaaag tatcatagca
    24541 catttacgga tacagtatat accaggtaat attcttatat ttcaacaaac tgatattgta
    24601 ttttttccct gatacattga ttgaagattt ttttaatgta ttttcatcaa gcttcaatgt
    24661 ccctcgttga attatgttaa atataacctc accgttagtt tcagcaatta ttgaatcagc
    24721 aggcaatatg tcataaaact tgttctgatt gatcatccca atacagaatc ccttgtttct
    24781 atcatctaat tgtataggat agcatacaca aaaactgtaa ttgcctgaca taggaggata
    24841 ctcaaagggt ttaaaaatag ctatatcatc atacggaaga tttgagacaa agtaatataa
    24901 cacgggagat agttgcgata atcttctgcc ataaagatca atgtttttca tggaataaag
    24961 tattgtataa gagctattga ttataatcag atcatcatag aattgttctg ttacattgct
    25021 tatcatctcg cttatatgct cgtattcaag cagataatac ttacgcccac cattatccat
    25081 gttcagaaga tgtgtgagat tggcaataag ttcatgtttt tgaataatag ttatagcatt
    25141 actcatatca ttaatatagg gggaaaacaa agctgaaata agctgtactt cagattttgt
    25201 tattgttttt gtactacaag atagtaaata taaaatcatt aatacagata cgcagagatt
    25261 tcttatcttc atatactttt gtgaatggac attataattt actttttaaa agctctcatt
    25321 taataatcgg tttatatttg agtaatcaca attataaaat aattttttac tatggatata
    25381 gtagaaattc taataatga
//
LOCUS       DAORYL010000042        24135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1742_length_24135_cov_13.222093, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000042 DAORYL010000000
VERSION     DAORYL010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 24135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24135
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1742_length_24135_cov_13.222093"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            254..658
                     /locus_tag="PLV81_08875"
     CDS             254..658
                     /locus_tag="PLV81_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10247.1"
                     /translation="MTITDIQIQENKKGIDIKNQYHLKYYDKINLGAFYTPTKYVDIV
                     WEMIKPFINNKSIVLDSSCGYGNFFDYKINCPKKANDIDYIATNQTKDNFPEIDVYNK
                     NALLNVNRKMFNISDNNELIIIGNPLIMILLQ"
     gene            691..1437
                     /locus_tag="PLV81_08880"
     CDS             691..1437
                     /locus_tag="PLV81_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10248.1"
                     /translation="MDIDIKTRDYGMSFLLSYAKLDADIICVLHPLSYLIKKSNFNLL
                     KNFTKNYKLVNGVIIDSGTFKETSKSISFPIIIALYKKEKTGMDYNYVQNYKFKTIDN
                     KNFSLNDFDFISNYINKYPLKNIKPNENDILFWTMRDINALKRNRTFIHEFGYNAIIV
                     DKNKLDYYIYVDVFKQFSHLIPYYFGNLDVFINNEMFNKYKDYFIYECIMRNNFLKKY
                     VQYDDNISLEKAKEIIINYFKFLTGEHYVD"
     gene            1540..2847
                     /locus_tag="PLV81_08885"
     CDS             1540..2847
                     /locus_tag="PLV81_08885"
                     /inference="COORDINATES: protein motif:HMM:NF025126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOM10249.1"
                     /translation="MRRRVVDYEEKTGINLFEKVFVHLRDDYIKKFGISQKVKRIDST
                     LIGSNIRRLNRLKLFLEVLNYFLKHSDESILERLSEEIKVYKDITPENYVFSLTNDDA
                     RMKIQEIAQYLYRIKVLFAHDSVSESQAYRILARLVDEQIEITGKGKGVKVKVSNELR
                     SSCIQSPYDPDATYREKGKQASQGYSVSIVETCDPDNKLQVITHVITEKNNVDDSTIL
                     EDNFEAIVGEDTEELIADGAYANKSVQDRLSESKKTLITTAIRGRKPDSNKVRSTDFE
                     IENNRIIRCPYGEIPIHQEYNNGVIIAHYAHESCSSCPLKCMIRKNKRKPHVLVITQE
                     RLQMDRQREKYNDSEYLKKCALRPAIEGTMFQFKLHLRNGKSRYRGKSKVKCSSILRT
                     IAINFKRVYAYRLKEALSYFILRNSMIIKSFLARKNILCCFST"
     gene            2969..4000
                     /locus_tag="PLV81_08890"
     CDS             2969..4000
                     /locus_tag="PLV81_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10250.1"
                     /translation="MEKYTMFSNTDFHFGHTSINEDIAYFYTKMNDAIINLVRSLPVS
                     VQTSAMIFLMHNAGIQPGQNLDFFSNYYSPIWSIIPHIMIRHTCTRIKQEFMYLAATA
                     HAMVMLLHSLDNHINDGQVVPDHLTLLIRSQGWKLFWDTISTFCSLIHTDKEIVNSCL
                     DRYYACITTDKSIDNLEEYLRIFRDQMATALIVPLLTVQFVQDMSLDEDIRALLECFG
                     LAWRIYDDIQDMKDDLNNGRNSAVTLSLPREVRCRWNEFLKIGTIIPEGENISERKND
                     SINILEEMIQTSGVIPILTKRIIKELKAAAKHAEDIGLFGLSDEIVKLRIHFVQGMKC
                     DTGEDNVSD"
     gene            3987..5027
                     /locus_tag="PLV81_08895"
     CDS             3987..5027
                     /locus_tag="PLV81_08895"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOM10251.1"
                     /translation="MSVIDSIPFEILEAVKKENLSIEVTTRCTSNCTYCFVLSDCIEY
                     HDLDYETVFSIIMEGYEIGYRHLHITGGEPLLWQPLFDLLHDAVTMGYESIFINTNGF
                     LLDSTIVQKLARIKGLSLSISLQGFLVVHETFRGNDSFLKATRGIEMALHYEIPVHIF
                     CVIGKSLLQHLPHFVQWVYQTFPGIRGITLIQLIRAPDTVTQLSNELLTPDDFIALVR
                     MISFLNIYGYCISLLENPLATVVAHLLQLPFFSNPPHLIRPGRLTVKADKSITIAHSS
                     RQLLGMYSHGALSHILTSQAYMQAVEEDDEICACCNYGEACRTATMQRPSEPFRYIDD
                     DIPYCKRVLDSI"
     gene            5354..6358
                     /locus_tag="PLV81_08900"
     CDS             5354..6358
                     /locus_tag="PLV81_08900"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOM10252.1"
                     /translation="MKACVTGATGFIGGNLVKALLQKKHSVKALVLPNDPKMPELKKN
                     KVEVVQGDIRDLESVKKVVKGCDVVFNCAAVVTDWAPQKLFEEVTVGGAKNVCEAALW
                     AKVKRVVDISTNDVFGLREDVIMDESFPLEPWGEPYPDYKIKAEEVMWRYHREKKLPV
                     TMVYPCWVYGPGDQTFVLLLADAIIKRELIFWRKDVLVWPTYVENLVDLLLVIAVDKR
                     AIGNGYLVHDGESVTLQKFCEGIANALGVKPIKTHIPYWTAYMAAVVMEAVWKLFTIE
                     KRPLLTTYTVKNLGSRLRFSIDKAKRELGWVPKISFKEGFERTMQWLKTLDLESLKIK
                     "
     gene            6394..7224
                     /locus_tag="PLV81_08905"
     CDS             6394..7224
                     /locus_tag="PLV81_08905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOM10253.1"
                     /translation="MESFAGKTAFITGGSSGIGLSVAKLLSKEGANIAIFARNRVRLE
                     EAMKEIEQAKKRHGQRFAWYQCDVSDNAQVKKAFTQALRQFDTCDILINCAGRAYPKY
                     FEDISYKQFEETMKINLFGIWNTCSFLVPHMKQKGGYIVNTSSLAGLVGVFGYSDYSA
                     SKFAIVGFSEVLRSELKRYNITVSVLCPPDTDTPGFEVENRTKPDETKEISKSARVMS
                     PDEVAQALLKGMKKKKFIILANFDSKLTWIAKRYAPWLVEAVMLSQIKKVQKKKGTSK
                     "
     gene            7239..7643
                     /locus_tag="PLV81_08910"
     CDS             7239..7643
                     /locus_tag="PLV81_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10254.1"
                     /translation="MSLKTTAYLAIVTGIGILLFWIAFFTVGLAPENPPACYFAYEHS
                     FPLPDTVLAIALIIGGILAIKQHDRALYLLLPASGGLIFLGLLDMAFNWQNGMYTITV
                     MDGVLNAFINIWCVVFGLCAILSLQREFVKKS"
     gene            7701..7775
                     /locus_tag="PLV81_08915"
     tRNA            7701..7775
                     /locus_tag="PLV81_08915"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7736..7738,aa:Val,seq:gac)
     gene            7874..9778
                     /gene="thrS"
                     /locus_tag="PLV81_08920"
     CDS             7874..9778
                     /gene="thrS"
                     /locus_tag="PLV81_08920"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOM10255.1"
                     /translation="MATVQLNNGKTLEIQDGKTLLDVIAKLNGKSKPVAARINGALKD
                     LRTAVTGTTQVELISFDSAEGRDVFWHSASHLMAQAVKRLFPEVKVSIGPAIDTGFYY
                     DFDKPGGFTQEDLEKIEEEMRKIVTEDIPIIRKELPRKDAIDLFSTLGEIYKVELLQE
                     LSDDIVSLYEQGDFVDLCRGPHLPSTGYIKAFKLLSIAGAYWRGDEKNKMLQRIYGVA
                     FPDETMLKEHLAFLEEVKKRDHRILGKELDLFSIHEDAGGGLIYWHPKGGRLRAILEQ
                     WWREEHFKNGYEILYTPHIGRATLWETSGHLDFYKENMYSPMDIDGNDYYIKPMNCPF
                     HIKIYQTSLHSYRDLPLRWAELGTVYRYEKSGVLHGLLRVRGFTQDDAHIFCTPEQME
                     DEVREVLRFSLYMWKVLGFSNIKAYLATRPQKSVGEEKRWIDAQESLKKAVQAEKLDI
                     EVDEGGGAFYGPKIDLKVKDALGREWQMTTIQFDFNLPDRFDLYYIGSDGQKHRPYMI
                     HRALLGSIERFIGILTEHYAGRFPVWLSPVQIILVNVGPEQAEYTKDLALKLRLQGIR
                     VKTDLRDETIKYKIRDAIEQRVPYIGVIGGREMQENTIAVRKRGEQKPETMKTDEFVA
                     FIQRQIANKQ"
     gene            9996..10553
                     /gene="infC"
                     /locus_tag="PLV81_08925"
     CDS             9996..10553
                     /gene="infC"
                     /locus_tag="PLV81_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOM10256.1"
                     /translation="MDRVDTKRFRVNEEIRASMVRLVGEDGGPQVIPLQEALQKAQDK
                     GLDLVEISPNQDPPVVKIIDYSKFKFEQIKKAKEAKKKQKVIQVKEIKLRPSIDLHDF
                     QHKVRHAREFIEEGNKVKFTVMFRGREVVHSDLGFKVLEDVKQALEDVALVETNPSKE
                     GRNITMIMAPMSAAQKKRIQEHNNQ"
     gene            10571..10768
                     /gene="rpmI"
                     /locus_tag="PLV81_08930"
     CDS             10571..10768
                     /gene="rpmI"
                     /locus_tag="PLV81_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004696338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOM10257.1"
                     /translation="MPKLKTSSGAKKRFKVTATGKIKRAKGWKSHLLESKSPKRKRAL
                     RKASYVSDSDAKRFARLLPYS"
     gene            10787..11143
                     /gene="rplT"
                     /locus_tag="PLV81_08935"
     CDS             10787..11143
                     /gene="rplT"
                     /locus_tag="PLV81_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOM10258.1"
                     /translation="MPRATTGKVHHKRREKILKDVKGFYGARKNLWRTAKDARRKKLQ
                     NSYKDRRRKKRDFRALWIMRINAAARLYGISYSRLIDGMKKANILIDRKMLAELAVSD
                     TNAFKAIVEKVTNKAA"
     gene            11158..12084
                     /locus_tag="PLV81_08940"
     CDS             11158..12084
                     /locus_tag="PLV81_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10259.1"
                     /translation="MLFKQEIISAIIMALFVFMVTISSSKALGRPLTPSMYTVDEGSI
                     EGYLGIDVIQTKSTFSKENMHVQTGILPGSDIRFSASLLQSNVHELSNNTIGDCAISI
                     WHSFGLLCNIVAGGMLIELGLPTAPDAYYNEKTFPTELGFTTFSLHFVAAYNNHSMTL
                     LFSGCYTFIPEPGESVFEGLSFNVTRSSTWRSLFGLNPLRKDSMLYYRRLKDDIASFS
                     LTAVYEMFPVIPYLSFCLGDRINNNPVYGEHGLPVLYKGYNDTLFLVTALGVRYFFSE
                     KTYIGTGLVYNVLRDYDYSLHYAAGIEASSVW"
     gene            12348..12986
                     /locus_tag="PLV81_08945"
     CDS             12348..12986
                     /locus_tag="PLV81_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10260.1"
                     /translation="MITMQQLEELESRIIKALQLIGDLRAENAKLESDNESLKVQAEE
                     ARLSLEEKEQEIARLKKEIENATNELAQIKQREEVLEKKIIEMLGKLDTLKSGKAPAF
                     EAKPQQQPVQPNPQRPEPKPVEEFLEEKPADEEDIIIIDDTQSSKGDVEVETLPTEED
                     EIVLLDDTDEEIVIDDVDGDLIIIDEETTEKKEAKDKISDDEDFIIIEDDEK"
     gene            12983..13324
                     /locus_tag="PLV81_08950"
     CDS             12983..13324
                     /locus_tag="PLV81_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002632026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOM10261.1"
                     /translation="MISGVHNPNKVKVTILGQVYTIEGDASADYIARVADFVNAKMED
                     VASNITNASPLQIAILAALNIADELFQIKESEYMATDEIREKTNMLISMLDEGLIGDV
                     FSVSGKSRQHL"
     gene            13415..13984
                     /locus_tag="PLV81_08955"
     CDS             13415..13984
                     /locus_tag="PLV81_08955"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOM10262.1"
                     /translation="MSQTFKALTDEELVKCVIDGNDQAFEELYQRYSERIFKLLYSYV
                     YNEEDAIDLMHDVFIRAYRHLHKFDIKRTFSSWIYKIAINCAKNHRYKVRKTDTMIER
                     EEQRLIQAEGVKTPEDYIIDNELTQEFSLAVDTLTDKFREVFLLRFGQQLQYSDIATI
                     LNISERTAKWRMERAIMYITDFLKKRGAI"
     gene            14041..15846
                     /locus_tag="PLV81_08960"
     CDS             14041..15846
                     /locus_tag="PLV81_08960"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOM10263.1"
                     /translation="MNRWADDKELYSEIAEYAGDSADALFSLLHNWQSSLPSLRDTCT
                     YKDIIEQSRKSKSYSKQWIPIVATGIAAVIVIGIILFNRFVEKPSQAPAICKAAIGNV
                     LVQRGHEYINLTNGMEVLQSDTIVCNKGSMAVIEIGSATLRIDENSKVSIDSLQKTQT
                     LSLAASMDKGILYATVQKLQKGDSILVKTKTAVAGIRGTSFLVKSDSKSTRLFVIDGK
                     VLLSPRLQDSKQEDITGVVVDGGKSCVIDTRTLRNIQKAITEKKMPLDEAIQQSIPVS
                     IADEKTMQVLHALNQDVSAQVVINEDKPSSINDLYPITSITPYRNDIVVTTNDSIYYC
                     ENDRIQWMHEYNPVSKPYVWDNFIVFQSKMLQALDTKGNSKWEIEIEGSMIGDNIVPI
                     RDKLVVPTTKGIIYFISKNGTILHKVNCDAPFVTRPVSFGQMVCVVTADGYLYAIDVV
                     LGVSIYRKYIGKVLDNGIFANYPEMYIVTPASIQKIHLLRDEIIWKYDNSGIVAAVEH
                     PQGILFATAHGTLGKIAANGTLEWQINPGKEIRSLQYTPKGIVCIAENVFYQISDAGE
                     VLWSYTLPALNSEILSVTPKTVYIRLGNSFLALRL"
     gene            16084..16581
                     /locus_tag="PLV81_08965"
     CDS             16084..16581
                     /locus_tag="PLV81_08965"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOM10264.1"
                     /translation="MNNELLNIAAKTAESVIDDTDSLDILQLVSFKLDEIEYGIDILS
                     VHEILRIPEITRLPNTPEYIKGVINLRGNVIPVVDIRLRFGMQQVALTELSRIIVVEI
                     GEKLVGLLVDNVHQVIRLSRSRIDEPHDLIEGISTEFISGIGRLQDRLIVILRLDNIL
                     FSDEQ"
     gene            16605..19670
                     /locus_tag="PLV81_08970"
     CDS             16605..19670
                     /locus_tag="PLV81_08970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOM10265.1"
                     /translation="MGFSLGEYQDMFLEEADELLQELNQNLLKLEKNPSDDEIINNIF
                     RAAHSLKSSAAFVGLTDLGDLAHKMENLLQGIRDKVMKVTPAIVDVLFQCFDVINDVI
                     REVSSGNPPSKDFSSLIKKIESVGKQGNIQEEKKEAPAPKRIQLSTNIIKDIRDGINN
                     GLSCCEIAITLDPDAAMKSLKVQLVLTNLEKIADILITIPSIEEVSMPEFDNRVQIIL
                     LTSATVEELKKACEVDQVTRIDLRKIELVKKDNKVALKYHEQETLFEEKPEADTKARD
                     VEEDVIPDEEEKDEIIIRSKDDKKTLQLRTVKVSVEKLDQLLNNVGELVIANSGFYKL
                     YEEMRRLNIEKSIINEFKNRMEQMSRIAKDLQSGIMKTRMVPIGQVFSRFNRLVRDLA
                     KEFDKKVVLEIKGEETELDKKVIDVIGEPLMHLIRNAVDHGVESVEERRQQKKPEIAT
                     ITLNAYQGGNQIFVEVSDDGKGLDLKKIKAKAIEKGLASPEMIANMDKEEIYNFIFTP
                     GFSTSDVVTDISGRGVGMNVVQETVNELNGSVAIESEPGMGTRFILSFPLTLAIIPAI
                     MVRVQRELYAIPLSDVIETIKILPEEITTIEGHEVINLRGEILSLIRLNNFVGIESGL
                     KGNERMPVVVVGYGNRKIGIVVDLLEGKQEIVIKSLEQNYRTVAGLAGASILGDGSIC
                     LILDISSMINKVIMQQDTFSFKEKQRVLGAKQASVEVVKTEVATKEQPVKETVDVQSK
                     KEIGETLLAKEMPIKEDIVFKKEPEEIVEVEEYKEPEKNYELKRGKDTVIEEKVHSVL
                     EEFKTELKEKIKDTLDKKDEIYQKLGISDDDLAKVQVLANVGITDAAESLSKIINRRV
                     DLSIPDVSVLAIESIPQSFGDVDSVYLGVYMPIVGQINGTILFSLREDSGYELVDMLL
                     GLPQGTTDTLTEDGESVLKEVTNIVGSAVVNAFADKIEMAIKPTVPSVVHDYMQSILD
                     SILAFHTAENEYALVMDTEFFHQDDRVIGKLLIMPTTSSLATIVERLRN"
     gene            19676..20722
                     /locus_tag="PLV81_08975"
     CDS             19676..20722
                     /locus_tag="PLV81_08975"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOM10266.1"
                     /translation="MSEKIKVLVVDDSALVRKVISDILESDKEIEVIGTANNGKSAVF
                     KNLTLNPDVITMDIEMPIMNGLEALQEIIKTNPKPVIMMSVLTQYGAEATFKALEYGA
                     VDFIPKPSTVLAMSMDDIAELLITKVKAVAKASLNLHVTKSEHVSETNKVSIIARHED
                     KKKIVGIGTSTGGPSALQRVLQKLPKNFPSPILVVQHMPPGFTKAFAERLDSLCNVSV
                     KEAEDGEKVLNGCVYIAPGDAHIVVEKTGDTRVIRLSQAEKVSGHRPSADVLFHSLAK
                     EDSHNVIGVIMTGMGRDGARGIKEIHDNGGYTIAQDESTSVVYGMNRVAVEMGGVDAV
                     VDVDSIPEEIISHI"
     gene            20942..21301
                     /locus_tag="PLV81_08980"
     CDS             20942..21301
                     /locus_tag="PLV81_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM10267.1"
                     /translation="MAKILIVDDAKFMRTLVKDALVPKGHEIIGEAENGVEAVQLYKK
                     LRPDLVTMDITMREKDGIEAAEEILQFDPNARIIMVTALGQENLLTRAIKIGVKDFVV
                     KPFPPERLQKAAEKALA"
     gene            21457..22206
                     /locus_tag="PLV81_08985"
     CDS             21457..22206
                     /locus_tag="PLV81_08985"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOM10268.1"
                     /translation="MITPDNNDVQCILHIENFDGPLDLLWDLIKKSKIEITQVSIADI
                     TEQYLEYLKLMESINIPVAMEFIVMASELLYYKSKALLPGADIGDEFFVPPLPPELIQ
                     KLLEYKKYQIASKNLLKLHEMQSDVFTRQIPPLSFEEDVYIEVNLFDLLNAFVDVLEA
                     EKETEQQRIVFDEILVSDRIMLLVSMLQDKDKIEFHDMFTQNPGRMEVVVTLLALLEM
                     TRIGAIAVLQHAQFGNIYIFKKDLNKVDTLH"
     gene            22324..22863
                     /gene="scpB"
                     /locus_tag="PLV81_08990"
     CDS             22324..22863
                     /gene="scpB"
                     /locus_tag="PLV81_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOM10269.1"
                     /translation="MGDSDDLFGIVEAILFVSAEPVPLQFFTRQLGIDATQAKIIIDS
                     LIDEYEDRDKGIKIIEISGGYQFVTNPKFASQLRQVMGYKKKETLSKGMLETLAIIAY
                     KQPIMLAEIEELRGVSSRMMVAALMRKNLIKPVGRKDVPGRPLLYGTTDEFLRYFGLN
                     KLSDLPKLSEIKEFNFEEE"
     gene            22865..23911
                     /gene="pheA"
                     /locus_tag="PLV81_08995"
     CDS             22865..23911
                     /gene="pheA"
                     /locus_tag="PLV81_08995"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOM10270.1"
                     /translation="MDTASFEKNIIESLSQLTELYSKELQNIMKEGEQGIFPVKAKII
                     SHLQQYARPPLTSELIEKIATEIFSVALSAARPLKVAYLGPVGTFTHIALLEIFGDVI
                     EAVPHRTISDVFDEVETGRATFGVVPIENSTEGAVTYTMDEFLDTDLKIVSEKSLRIT
                     YSLLSVCDSIEKIKKIYSHPQSLAQCKNWLKANLPNVEINQVNSTAKAAETASWDKYS
                     AAIASNISAKIYNLNIIADGIEDSRQNYTRFLVLGKHDNPPTGNDKTSIVCAVKDQPG
                     ALYKLLKPFSEHGINMTMIESRPDKKKMWAYNFFIDFNGHRTDPVVQKALEAMKQETI
                     FLKVLGSYQAEKHD"
     gene            23942..>24135
                     /locus_tag="PLV81_09000"
     CDS             23942..>24135
                     /locus_tag="PLV81_09000"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037053.2"
                     /note="catalyzes the formation of
                     3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate from
                     phosphoenolpyruvate and D-erythrose 4-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOM10271.1"
                     /translation="MIIVLKPNVTQDEIDHIVGKIKSLNLQPHVSKGQYRTIITVIGD
                     EDIIREQPLEAISGVESVKP"
BASE COUNT         8156 a         3689 c         5168 g         7122 t
ORIGIN      
        1 cgatccgcta acagcggcta tacgccatta aaacggcgca tagccgcgaa ccgttaggcg
       61 acatattttt gaaaaatttg ttaattgaaa tgtttttggt ggactttaag aaataaggaa
      121 tttgattttt ttaattttta aagagggggg tataaggtgt tttctccgct atcgatccaa
      181 aaacgaattt taattggaaa aaagcaaaaa gatttatata cttaaattag ttttaactta
      241 ttaaggagat aatatgacaa taacagatat acagattcaa gaaaataaaa aaggtattga
      301 tattaaaaat caatatcatt taaagtacta tgataaaata aatcttggtg ctttttatac
      361 tccaacgaaa tatgttgata tagtatggga aatgataaaa ccttttataa ataataaaag
      421 tattgtttta gatagctctt gtggatatgg aaattttttt gattataaaa ttaattgtcc
      481 gaaaaaggca aatgatattg attatattgc tactaatcaa acaaaagata atttccctga
      541 aattgatgtt tataataaaa atgctttatt aaatgtgaat agaaaaatgt ttaatatctc
      601 tgataataat gaattaatta ttattggaaa cccccttata atgatactac ttcaataata
      661 agaagaggta taaaaaatga taatttagaa atggatattg atataaaaac aagggattat
      721 ggaatgtctt ttttgttatc atatgcaaaa ttagatgcag atataatttg tgttttacac
      781 cctttgtctt atttgattaa aaaatccaat tttaatttat taaaaaattt tacaaaaaat
      841 tataaactag ttaatggagt tattatagat agtggaacat ttaaagaaac ttcaaaaagt
      901 atttctttcc caataataat agctttatat aaaaaagaaa agactggaat ggattataat
      961 tatgttcaaa attataaatt caaaacaatt gataataaaa acttttcctt gaatgatttt
     1021 gattttattt ccaattatat taataaatat ccattaaaaa acattaaacc aaatgaaaat
     1081 gatatattgt tttggacaat gagagatata aatgctttaa aaagaaatag aacttttatc
     1141 catgaatttg ggtataatgc tattattgta gataaaaata aattagatta ttatatttat
     1201 gtagatgtat ttaaacaatt tagtcattta attccatatt attttgggaa cttagatgta
     1261 tttataaata atgaaatgtt taataaatat aaagattatt tcatttatga atgtataatg
     1321 agaaataatt ttttaaaaaa atatgttcaa tatgatgata atatttcttt agaaaaggca
     1381 aaggaaatta taataaatta ttttaaattt ttaactggag aacactatgt agattaaaaa
     1441 aattaattag gcaggtttca ttttgatcat ttgtttatac tggcgatggg gttaaacagg
     1501 attggtgaga ggacaatatc agaacgagca ttttattata tgcgccgacg tgttgttgat
     1561 tatgaggaaa agacagggat aaatctattt gagaaagtat ttgtacattt gagggatgat
     1621 tatataaaga aatttgggat tagtcagaaa gtcaagagga ttgattcaac gttaattggt
     1681 tcaaatattc gcagactaaa ccggctcaaa ctgtttttag aggtgttgaa ttattttttg
     1741 aagcatagtg atgagagtat tttggagagg ttatcggaag agataaaggt atataaggat
     1801 ataacacctg agaattacgt attttctctg acgaatgacg atgcaaggat gaaaattcag
     1861 gagattgcac agtatcttta ccgtatcaag gtgctatttg cccatgattc tgtgagtgaa
     1921 agccaggcat atcgaatact tgcacggctg gttgatgagc agatagagat aacagggaaa
     1981 gggaaagggg taaaggtaaa agtttcaaac gaactaagga gcagttgcat acaatcaccg
     2041 tatgatcctg atgcgacata tcgggaaaaa gggaagcagg caagtcaggg gtattcggtt
     2101 tcaatagtag agacatgcga tccagacaac aaactccagg tgattacgca tgtgataaca
     2161 gaaaaaaaca atgttgatga cagtacaata cttgaagaca attttgaagc aatagtgggg
     2221 gaggacacgg aagaacttat tgccgatgga gcatatgcaa acaaaagcgt acaggacagg
     2281 ctatcggaat caaagaaaac tcttattaca acagcaatac ggggaagaaa gccagacagc
     2341 aacaaagtac gaagtactga ctttgagatt gaaaacaatc gcataatacg ctgtccctat
     2401 ggggaaattc cgatacatca ggaatataat aatggtgtta tcatagcaca ctatgctcat
     2461 gagagttgtt cctcctgtcc actgaaatgt atgatacgga aaaacaaacg taagccgcat
     2521 gtgcttgtga ttacacaaga gagattacag atggataggc agcgagagaa atacaatgat
     2581 agtgagtatt tgaaaaaatg cgcacttcga ccggcaattg aaggtacaat gtttcagttc
     2641 aagctgcatt tgagaaatgg gaaaagtcgg tatagaggga aaagtaaggt aaaatgcagt
     2701 tcgatactga gaacaattgc aataaatttc aaaagagtgt acgcttacag gcttaaggaa
     2761 gccttatcct attttatttt aagaaatagt atgataataa agtcattttt agcacgaaaa
     2821 aatattcttt gctgtttttc tacttaaaaa cagtgtgttt gtgcagggga atcatatatt
     2881 aataattaac ttgtcaaaaa tatgtatttg gataatacta aataccctta gggttcatgt
     2941 ttctgcaaca aagcagttag taattgtaat ggaaaaatat acaatgtttt caaatactga
     3001 ttttcatttt ggacatacat cgataaatga agatatagca tatttttata caaaaatgaa
     3061 cgatgctata atcaaccttg ttcgttctct tccagtatcg gttcaaacct ctgcgatgat
     3121 atttttaatg cataatgctg gcattcagcc tggacaaaat ctggattttt tcagcaatta
     3181 ctattcgcct atatggtcaa tcattcctca tatcatgata aggcatacat gtacacggat
     3241 taaacaggag tttatgtatc tcgctgcaac agctcatgca atggtcatgc ttcttcattc
     3301 tttagataat catattaacg atggtcaggt tgtaccagat catttgactc tccttattcg
     3361 aagtcaggga tggaagctat tctgggatac catttcaact ttttgttcac ttattcatac
     3421 agacaaggaa atagtgaata gctgtcttga tagatattat gcgtgtataa ccacggataa
     3481 aagcattgat aatctggaag aatatctacg tattttcagg gatcaaatgg ctacagctct
     3541 cattgttccc ctgctgactg tacaatttgt acaggatatg tctttagacg aagatatcag
     3601 agccttactt gaatgttttg gtttagcctg gcggatatat gatgatatcc aggacatgaa
     3661 ggatgatctg aataatggac ggaattctgc agtaactctt agcctgccca gggaagttcg
     3721 ttgtcgctgg aatgaatttt taaaaatagg aaccatcata cctgaagggg aaaatatatc
     3781 agagcgtaaa aatgatagca taaatattct tgaagaaatg atacagactt caggtgtcat
     3841 ccctattctt acaaaaagga ttattaagga attaaaagct gctgcaaaac atgcagaaga
     3901 tattggcctt tttggccttt cagatgaaat agtgaaatta cgcattcatt ttgttcaggg
     3961 gatgaaatgt gatactggag aagacaatgt cagtgattga ttctatacca tttgaaattc
     4021 ttgaagctgt aaagaaggaa aacctttcaa tagaagttac aacacgatgt accagcaact
     4081 gtacctattg ctttgtacta tctgattgta ttgaatatca tgatcttgat tatgaaacag
     4141 tattctcaat aataatggaa ggttatgaaa ttggctatcg acatcttcac attactggtg
     4201 gtgaaccgtt attatggcag cctctttttg atctactgca tgacgcagtt acaatgggat
     4261 atgagtcaat atttataaat acaaatggct ttttgttaga tagtaccata gtacaaaagc
     4321 ttgctagaat aaaaggtctt agcctttcaa tcagcttaca gggttttttg gtggtacatg
     4381 aaacatttcg tggtaatgat tcttttttaa aagctacccg tggaattgaa atggcattac
     4441 actatgaaat tccagttcat attttttgtg ttattgggaa aagtctttta cagcatctgc
     4501 cccattttgt tcaatgggtg tatcaaacat ttcccggtat aaggggaata acattaatac
     4561 agcttatacg tgcccccgat actgttacac aattatccaa tgaactattg acacctgatg
     4621 attttattgc tttagtacgt atgatctcat ttttgaatat atatgggtat tgtatttcac
     4681 tgcttgaaaa cccacttgct accgtagttg ctcacctgct acagttgccc tttttttcca
     4741 atccacctca ccttatacgc ccgggaagac ttaccgtaaa ggctgataaa agtattacca
     4801 ttgcccattc atcgcgtcaa ttattgggta tgtatagcca tggagcactt agccatatcc
     4861 ttacatcaca agcatacatg caggcagttg aagaggatga cgaaatatgt gcatgctgca
     4921 attatggaga agcatgtcgc acagcaacaa tgcagcgtcc atcagagcct tttaggtata
     4981 tagatgatga tatcccctat tgtaaacgtg ttctggatag tatataatga ctatctctgt
     5041 gctatttatt gttatgcaga aaaattgggg tcagagcctc tgtgtttgcc tctcccaaaa
     5101 atagggtcag agcttttgcc aggccaagac gaagccattt cccctttgtg aaaaaaatca
     5161 aattgaattg ttgccacatg gacaaaagaa catgcatgaa gggaatgggg tcagagcata
     5221 tattaattgc ttgcaatttc tgtggaattt gagaaagttt aacaaattat agaggtttat
     5281 ccataaaaat tgcacgtacc aactaccttg atgaaaaaaa gattgtgata atagaattat
     5341 taaggaggtt accatgaaag catgcgttac aggtgcaact ggatttattg gaggaaattt
     5401 agtaaaagca ttgcttcaaa aaaaacattc agtaaaggca ctggtattac ccaatgaccc
     5461 aaaaatgcca gaactaaaaa agaataaagt agaggtggtg cagggcgata tcagggatct
     5521 tgaatctgtt aaaaaagtgg ttaaaggctg cgatgtagta tttaactgtg cagcagtagt
     5581 gactgactgg gcaccgcaaa aactatttga agaggttacc gttggtggag caaaaaatgt
     5641 ttgtgaagct gcgttatggg caaaggtaaa gcgggtagtt gatatttcca ccaatgatgt
     5701 ctttggctta agggaagacg taattatgga tgaatcattt ccgcttgagc cctggggtga
     5761 gccatatccg gattataaaa taaaagctga agaggtaatg tggcgctacc atcgtgaaaa
     5821 gaaattgccc gtaaccatgg tatacccttg ttgggtttat ggcccgggtg accaaacatt
     5881 cgtactgctt ttggctgatg caataataaa acgtgaactc attttctggc gcaaggatgt
     5941 actggtgtgg ccaacctatg ttgaaaatct agtggattta ctgttggtga ttgcagtgga
     6001 taagcgggcc attggtaatg gctatctggt gcatgatggt gaaagtgtta cattgcaaaa
     6061 attttgcgaa ggcatagcca atgcattagg tgtaaaacca ataaaaaccc atatacccta
     6121 ctggacagca tatatggcag cagtggtaat ggaagccgta tggaaattat ttaccataga
     6181 aaagcgcccg cttcttacaa cgtatactgt aaaaaattta gggtcgcgac tacgatttag
     6241 cattgataaa gcaaagcggg aattaggctg ggtaccaaaa atatcgttta aagaaggatt
     6301 tgagcgcaca atgcagtggc ttaaaacact tgatttagaa tcattaaaga taaagtaaaa
     6361 aaaagtaaat acacgtaaag ggagatagta actatggaaa gttttgcagg taaaacagcc
     6421 tttattactg gtggttcaag tggcatagga ttatcggtgg caaaactcct ttcaaaggag
     6481 ggtgctaata ttgctatttt tgcccgcaac agagtccgac tagaagaagc aatgaaagaa
     6541 attgagcagg caaaaaagcg ccatggccag cggtttgcgt ggtatcagtg cgatgtatcg
     6601 gacaatgcac aggttaagaa agcttttact caggccttac gtcagtttga cacatgtgat
     6661 attctcatca attgtgctgg cagggcctat cccaaatatt ttgaagatat aagctataag
     6721 cagtttgaag aaactatgaa aataaatttg tttggtatat ggaatacctg ttctttttta
     6781 gtgccgcata tgaagcaaaa aggcggctac attgtcaata catcatcgct ggcagggctg
     6841 gtaggtgtat tcgggtatag tgattacagc gcatcaaaat ttgccatagt tgggttttcg
     6901 gaagtattac gaagcgagct caaacggtat aatattaccg tatcggtgct ctgtccccct
     6961 gacactgata ctcccggctt tgaagttgaa aacagaacaa agcctgatga aaccaaagag
     7021 atatcaaagt cagccagggt gatgtcacct gatgaagtgg cacaggccct gcttaaagga
     7081 atgaaaaaga agaagtttat aatccttgca aactttgaca gtaaactcac ctggattgcc
     7141 aaacggtatg ctccatggct tgttgaagca gtgatgcttt ctcagataaa aaaggtgcaa
     7201 aagaaaaagg gaacatcaaa ataataacag gaggttttat gtcattaaaa acaacagcat
     7261 atttggcgat agttaccgga ataggaatcc ttttattctg gattgccttt ttcacagtag
     7321 ggcttgcccc ggaaaatccg cctgcatgct attttgccta tgaacattcc ttccccctgc
     7381 ctgatacagt gttagcgata gctcttatta ttggaggaat cctggcaata aaacaacatg
     7441 acagagcttt gtatctgctt ttgcctgcat cgggtggttt aatctttttg gggttacttg
     7501 atatggcgtt taactggcag aatggcatgt atacaatcac cgttatggat ggcgtactca
     7561 atgcgtttat taatatttgg tgtgtggttt ttggattgtg tgctatactg tcactacagc
     7621 gtgaatttgt gaaaaagtct tgacaggcaa taagctcttt cgtatttaca tttcatcctg
     7681 aacgtaatca ctatttttat gggcgattag ctcagcctgg ttagagcgcc tgctcgacac
     7741 gcaggaggtc actggttcaa ctccagtatc gcccaatttt tttattatta aaggatgtaa
     7801 aaaaacaata gtgttgcaag gataagggac gtaacgctat ctcatctatg atttcattat
     7861 aaaggctttt acaatggcaa cggtacaatt gaacaacggg aaaacactgg aaatacagga
     7921 tggcaaaacg ctgcttgacg ttattgcaaa gcttaatgga aaaagtaaac ctgttgcagc
     7981 cagaataaac ggagcactta aggatttgcg tacagcagtt accggcacaa cacaggttga
     8041 actcatatcc tttgacagtg ctgaaggaag ggatgtattc tggcactctg catcgcacct
     8101 tatggcacag gcagtaaaac ggcttttccc tgaagttaaa gtgtctatag gtcctgccat
     8161 tgatacagga ttttactatg atttcgataa acccggtggt tttactcagg aagatctgga
     8221 aaaaatagaa gaggaaatgc gtaagatagt tactgaggat atacccatta tccgcaaaga
     8281 attaccgcgt aaggatgcca tagatttatt ttcaacattg ggtgaaatct ataaagttga
     8341 actgttacag gaactatcgg acgatattgt ttcattatat gaacagggcg attttgttga
     8401 tctatgccgt ggaccccatc tgcccagtac cggctacata aaggcattca agctattaag
     8461 tattgccggt gcatactggc gtggtgatga aaaaaacaag atgctacagc gcatttatgg
     8521 tgttgccttc cctgatgaaa caatgcttaa ggaacacctg gctttcttgg aagaggtaaa
     8581 aaaacgtgac cacaggatac tgggtaaaga gttggatttg ttcagcatac atgaggatgc
     8641 agggggaggc cttatatact ggcatccaaa agggggtagg ctcagagcaa tcttagaaca
     8701 gtggtggcgt gaagaacact ttaaaaatgg ctatgaaatt ctgtatacac cgcatattgg
     8761 tcgtgcaaca ttgtgggaaa cttcagggca tttagacttt tacaaagaaa acatgtattc
     8821 gcccatggat attgatggta atgactatta cataaagccc atgaactgtc ccttccatat
     8881 caaaatttac cagaccagcc tgcactctta cagggatttg ccgctacggt gggcggaact
     8941 tggcacggta tatcggtatg aaaagtcggg cgtgttgcat gggttgttgc gtgttcgtgg
     9001 atttactcag gatgatgcac atatattctg tactcctgaa cagatggaag atgaagtgcg
     9061 tgaggtgttg cgtttttcac tgtatatgtg gaaggtcctt ggattttcca atatcaaggc
     9121 atatctggca acaaggcctc aaaaatcagt aggcgaagaa aaacggtgga ttgatgcaca
     9181 ggaatcatta aaaaaagctg ttcaggctga aaagctggat atagaagttg atgaaggtgg
     9241 cggagcattc tatgggccaa aaattgacct taaagtaaag gatgcgttgg gccgtgaatg
     9301 gcaaatgacc accattcagt ttgatttcaa tcttcctgac cgctttgacc tttactatat
     9361 tggaagcgat ggtcagaagc acaggccata catgatacac agggcgctgt taggatcaat
     9421 tgaacggttt ataggcatat taaccgaaca ttacgcagga agattccctg tatggttgtc
     9481 gccagtacag attatactgg tcaatgtggg tcctgaacag gctgagtata ccaaagattt
     9541 agccctaaaa ttgcgtttac agggaatacg ggtaaaaact gatttacgtg atgaaacaat
     9601 aaaatataag atacgcgatg ctattgaaca acgagtcccc tatattggtg ttattggtgg
     9661 cagggaaatg caggaaaata ctattgccgt ccgtaaacgg ggcgagcaaa agcctgaaac
     9721 aatgaaaaca gatgagtttg ttgcatttat tcagcggcag atagccaata aacaatagat
     9781 gtgtaatatg tgcatttact ctgctttcag taatgcaagg gtgtacaggg tcgtgagtaa
     9841 cattttttat attttaataa gaattattgt tgattaatat ataacagtga taagatgtgg
     9901 aaaatttttt cgttgaaata ttttctataa tacggtatat agtatatcaa ttagtttata
     9961 aattttgtgt atagaaaaag tgtaaggagg gtaacataga ccgggttgat accaagcgtt
    10021 tcagggtaaa tgaggagata cgagcctcta tggttcgttt agttggcgaa gatggtggtc
    10081 cgcaggtaat tccgttacag gaagcattac aaaaagcaca ggataaaggc ttagatctgg
    10141 ttgaaatatc acccaatcag gatccaccag ttgtaaaaat aatagattac agcaaattca
    10201 aatttgagca aatcaaaaaa gccaaagaag ccaaaaagaa acagaaggtc attcaggtaa
    10261 aagagattaa attaagacct tctattgatt tgcatgattt tcaacataag gtgcgccatg
    10321 cacgggaatt cattgaggaa ggcaataaag taaaatttac tgtcatgttc agaggaagag
    10381 aagtggtgca cagcgattta ggattcaagg tactggaaga tgttaaacag gctttagaag
    10441 atgttgcgtt ggtagaaact aatccttcaa aagaaggcag gaatataacc atgataatgg
    10501 ctccaatgtc agctgcacag aagaagcgga ttcaggagca caataatcaa taagtgaaga
    10561 gaggtatacg atgcccaagt taaaaaccag cagtggagca aagaagcgtt ttaaagtgac
    10621 agctactggc aagataaaac gtgcaaaagg atggaaaagc catcttctgg aatcaaaaag
    10681 cccaaagcgc aaacgtgcat tgagaaaggc ttcatatgta tctgattcag atgcaaagcg
    10741 ctttgcacgg ttactgccat attcataata cagtgaggta cacattatgc cacgagcaac
    10801 aacaggtaag gttcatcata aaagaagaga aaagatatta aaagatgtta agggttttta
    10861 cggtgcacgt aaaaacctgt ggcgaacagc aaaagatgcc cgtaggaaaa agttgcagaa
    10921 ttcgtataag gatagaaggc gtaaaaaacg cgatttcagg gcattatgga ttatgcgtat
    10981 taatgcagct gcacggcttt acggtatttc atacagccgt ctcattgacg gtatgaagaa
    11041 agccaatata cttattgatc gtaagatgtt ggctgaactt gcagtaagcg ataccaatgc
    11101 tttcaaggca attgttgaaa aggtaaccaa caaagctgca tagtgatgta gttgattatg
    11161 ctgtttaaac aagaaattat aagcgccatt atcatggcgc tttttgtttt tatggttact
    11221 atctccagca gcaaggcttt aggcaggcca ttaactcctt ccatgtacac tgttgatgaa
    11281 ggttcaattg aagggtattt aggtatagat gtaatccaaa caaaatcaac atttagtaaa
    11341 gaaaacatgc acgtgcaaac aggcatattg ccagggtctg atatcaggtt ttctgcttca
    11401 ctgttacagt ccaatgtaca tgaactatcg aacaatacta taggagattg cgcaattagt
    11461 atttggcaca gttttgggct attatgtaat attgtagctg gtggcatgct cattgaattg
    11521 ggattaccaa ccgctcctga tgcatattat aatgaaaaaa ccttccctac cgagttggga
    11581 tttacaacat tttcattaca ttttgtggct gcatataata accattcaat gacactgctt
    11641 ttttccggct gttatacatt tataccagag ccaggtgaat cggtatttga aggactatcg
    11701 tttaatgtaa cacgttcaag cacctggaga agtctttttg ggttaaaccc attgagaaaa
    11761 gacagtatgc tttattacag gagattaaag gatgacattg cctctttttc tttaactgca
    11821 gtttatgaga tgttccctgt aattccatat ctgtcttttt gtttagggga taggattaat
    11881 aacaaccctg tatatgggga gcatggactg cctgtattgt ataaagggta taatgataca
    11941 ctctttctgg ttactgctct gggtgttcgg tatttcttta gtgaaaaaac gtacattggt
    12001 accggattag tatacaatgt attacgggac tatgactatt cactgcacta tgcagctgga
    12061 atagaagcat catctgtatg gtaatttttt ataataaaaa attaaaaaat ttctcttgat
    12121 tttatactgt gataattata ctttattgtt actggattaa agaataattt ggcgcattga
    12181 ttgccagtac attaaatggc gatcacatct tttgagtgtt ttcatatgat ccggaagcta
    12241 ttttttggtt taagggaata atattttagt ttattgaagt tttttttatg ccgacattta
    12301 atacagtaaa acttttttaa aaatgattaa ttcgggagga ttttctcatg attacaatgc
    12361 agcaattaga ggagcttgaa agcaggatta taaaagcatt acagcttatt ggagatctta
    12421 gagccgaaaa tgccaagtta gaatcagata atgaatcgct aaaagtacag gctgaggaag
    12481 cgagattgag cctggaagaa aaggagcagg aaatagcacg gcttaaaaaa gaaattgaga
    12541 atgccaccaa tgaacttgct cagattaaac agcgcgagga agtactggaa aagaagatta
    12601 ttgaaatgct gggtaaactt gatacattaa aatcgggtaa agctcctgca tttgaagcaa
    12661 aaccacaaca gcagccagtg cagccaaatc cacaacgccc tgaaccaaaa cctgtagaag
    12721 aatttctgga agagaagcca gcagatgaag aagatattat catcattgat gatacgcaaa
    12781 gctcaaaggg cgatgtagaa gttgaaacct taccaacaga agaagatgaa atagtattac
    12841 tggatgatac ggatgaagag atagttattg atgatgttga tggggatttg ataataattg
    12901 atgaagaaac taccgaaaag aaggaagcaa aagataaaat ttctgatgat gaagatttta
    12961 taattattga ggatgatgaa aaatgatatc aggtgtgcat aatcccaata aagttaaagt
    13021 aaccatactg ggtcaggttt atactatcga aggagatgca tcagctgatt atatagcaag
    13081 agttgctgat tttgttaacg caaagatgga agatgttgca agcaatataa cgaatgcaag
    13141 cccactgcag attgcaatcc ttgctgcgct gaatattgcg gatgagctct ttcagattaa
    13201 agaatctgaa tatatggcaa ctgatgaaat ccgcgaaaaa accaatatgc tcatttcaat
    13261 gcttgatgaa ggattgattg gagatgtttt cagtgtctcg gggaaatccc gtcagcatct
    13321 ataaatagat agtatgaatg aaataataaa tatccaaaaa attttttacc tgcattgcac
    13381 ttttaataca ttccagtgat atatactaat aacaatgagt caaactttta aagctctgac
    13441 agatgaagaa ttggttaagt gtgtgattga cggcaatgac caggcatttg aagagcttta
    13501 ccagcgttat tctgaacgta ttttcaagct tctgtatagt tatgtttaca atgaggaaga
    13561 tgcaatagat cttatgcacg atgtatttat cagggcttac aggcatttac ataagtttga
    13621 cataaagcgt acattttctt catggatcta taagattgca attaattgtg ccaagaatca
    13681 caggtataaa gtacgtaaaa ctgataccat gattgaaaga gaagaacagc gtctgataca
    13741 ggcagaaggt gtaaaaacac ctgaagatta tataattgat aatgagctga ctcaggaatt
    13801 ttctctggct gttgatacgt taacggataa atttcgagaa gtattcctgc tacggtttgg
    13861 gcagcaattg cagtacagtg atattgcaac aattttaaat atttcagaac gcactgcaaa
    13921 atggcgtatg gaaagagcca tcatgtatat cactgacttt cttaaaaaaa ggggagccat
    13981 ataaaatttc atgtacggct gggtagtaat agatacatat actgtgaatc taggattgtt
    14041 atgaatagat gggctgatga taaagaactg tattcagaaa tagctgagta tgcaggtgat
    14101 tctgcagatg cgctgttctc attactgcat aactggcaat catcattacc ttctttgcgt
    14161 gacacgtgta cgtataaaga catcattgaa cagtcaagaa aatcaaaatc atattcaaaa
    14221 cagtggattc cgattgttgc cacaggtata gctgctgtga ttgtaattgg tattatactt
    14281 tttaaccgct ttgtagaaaa accttctcaa gcacctgcaa tatgtaaagc tgctattggc
    14341 aatgttctgg tacaaagggg gcatgaatat attaatctta ccaatggtat ggaagtgtta
    14401 caatctgata ctattgtatg taataaaggg tcaatggctg taatagagat tggatctgca
    14461 acattgcgta ttgacgagaa ttctaaagtt tcaattgatt ctttgcaaaa aacgcagacg
    14521 ctatcgcttg ctgcttcaat ggataaaggt atactctatg caacagtgca aaaattgcaa
    14581 aaaggagata gtattttagt aaaaacaaaa actgctgttg caggtattcg tgggacttct
    14641 tttctggtaa aatccgatag caaatcaacc agactgtttg tgattgatgg caaggtgctt
    14701 ctttcaccac gcttacagga tagcaagcag gaagatatta caggtgtagt ggttgatgga
    14761 ggaaaatcat gtgttataga tacgcgcaca ctgaggaaca tacaaaaagc cattacagaa
    14821 aaaaagatgc cactggatga agcaattcag caatcaattc ctgtatctat agcagatgaa
    14881 aaaacgatgc aagtccttca tgcactcaat caggatgtga gtgcacaggt tgtcatcaat
    14941 gaggacaaac cttcaagtat taatgatctt taccccatta cttctattac accatacagg
    15001 aatgatattg ttgtaacaac aaatgacagc atctattatt gtgaaaatga tcgtatacaa
    15061 tggatgcacg aatataatcc tgtatccaaa ccatatgtat gggataattt tattgttttt
    15121 cagagcaaaa tgctgcaggc tcttgataca aaaggcaaca gtaaatggga gatagaaatt
    15181 gaagggagta tgattggtga taatattgtc ccgatacgag ataaactggt agttccaaca
    15241 acaaaaggca taatttattt tattagtaaa aatggcacaa tactgcataa ggttaattgc
    15301 gatgctcctt ttgttacaag gcctgtttca tttggtcaga tggtatgtgt tgttactgct
    15361 gatggatatc tgtatgctat agatgttgtg ttaggtgtca gtatttacag gaaatatata
    15421 ggtaaggtgc tggataatgg tatttttgcc aattaccctg aaatgtatat agtaacacct
    15481 gcttctatcc agaagattca tcttttacgg gatgaaatta tatggaagta tgataacagt
    15541 ggtatagtgg cagcagtaga acatcctcag gggatactat ttgctactgc tcatggtaca
    15601 ttaggtaaaa tagctgctaa tggaacgtta gaatggcaga taaaccccgg caaagaaata
    15661 cgttcattac aatatactcc aaaagggata gtatgtatag cagagaatgt tttttatcaa
    15721 atttcggatg ccggtgaagt attatggtca tatacattac ctgccctaaa ttctgaaata
    15781 ctatcagtaa ctcctaaaac agtttatatt cgcctgggaa acagctttct tgccttgagg
    15841 ctttaaatta caaaaaaatt gtcaaaccgc acatttctgc taacagaaat gataggcatt
    15901 ttagaaaaaa tcgttttttt attgaatatc tttacataat aattcataat aaaaggctca
    15961 aagatcctat aacaatgata ctaaactttt ttaaaagttg aataattttg tgttgaaatt
    16021 atattgaata tatagcatag tatatacaat tgtatataat caggtatagt ataagggtgg
    16081 attatgaata atgaattatt gaacattgct gcaaaaacag cagaatctgt aatagatgac
    16141 actgattccc ttgatatact tcagctggta agctttaagc ttgatgaaat tgaatatgga
    16201 attgatatat tatccgtgca tgaaatactg cgaataccgg aaattacacg tcttcccaat
    16261 acgcctgaat atatcaaagg agttattaat ctgcgtggta atgttatccc tgtggttgat
    16321 atacgcttgc ggtttggcat gcaacaggtt gcattaactg aactaagcag gataattgtt
    16381 gtggagatag gcgagaaatt agttggtctt ttggtagata atgtgcatca ggtgatacga
    16441 ttgtcacgct caagaattga tgagcctcat gatttgattg aaggcatttc aacagaattt
    16501 ataagtggta ttggccgttt gcaggacagg cttattgtaa tccttcgtct tgataatatt
    16561 ctattcagcg atgagcaatg attttttata tttgaggtga taaaatgggc ttttccttag
    16621 gtgaatatca ggatatgttt ctggaagaag ctgatgagct tttgcaggaa ctcaatcaga
    16681 atttactaaa acttgaaaaa aatccttctg atgatgagat tataaataat attttcaggg
    16741 ccgcacattc acttaaaagt tctgcagcct ttgtaggttt aactgatctg ggcgatctgg
    16801 ctcataagat ggaaaatctc ctccagggta ttcgtgacaa ggttatgaaa gtaacacctg
    16861 ctattgtaga tgtgttattt cagtgttttg atgttattaa tgatgtaata cgagaagttt
    16921 catccgggaa tcctccatcg aaagattttt catctttaat aaaaaaaatt gaatcagttg
    16981 ggaagcaagg gaacattcag gaagaaaaga aagaagctcc agcccccaaa aggatacaat
    17041 tatcaactaa tattattaaa gatatacgag acgggattaa caatggtttg tcgtgctgtg
    17101 aaattgccat aacccttgat cccgatgctg caatgaaatc attaaaagta caactggtct
    17161 taaccaatct ggaaaagatt gctgatattc tgattactat accttcaatt gaagaagttt
    17221 ccatgccaga atttgataac agggtgcaga ttattttatt aaccagtgct acagttgagg
    17281 aactaaagaa agcatgtgag gtcgatcagg ttacaagaat agacctcaga aaaattgaac
    17341 tggtaaaaaa agataataag gttgctctta aataccatga gcaggaaaca ctttttgagg
    17401 aaaaacccga ggcagatact aaggcaaggg atgttgagga agatgtaata cctgatgaag
    17461 aagaaaaaga tgaaataata atcaggtcaa aagatgataa gaagactttg caactaagga
    17521 cggtaaaggt ctcagtagaa aaacttgatc agctattaaa caatgttggg gaacttgtta
    17581 tagcaaattc aggcttttat aaattgtatg aagaaatgcg gcgtctgaat attgaaaaga
    17641 gtataataaa tgaatttaaa aaccgcatgg agcaaatgtc acgtattgcc aaggatttac
    17701 agagtggcat aatgaaaacc aggatggtcc ctattggaca ggttttttca aggtttaacc
    17761 gactggttcg ggaccttgca aaagaatttg ataaaaaggt tgttctggaa attaagggtg
    17821 aagaaacaga actggataaa aaggtaattg atgttattgg tgaaccactg atgcatttaa
    17881 tcagaaatgc tgttgatcat ggtgttgaat ccgttgaaga aaggcggcag caaaagaaac
    17941 ctgaaattgc aacaataaca ctgaatgcct atcagggtgg caatcagata tttgttgaag
    18001 taagtgatga tgggaaaggg ttggatctta agaaaattaa ggcaaaagca atagagaaag
    18061 gattagcatc acccgagatg atagctaata tggataaaga agaaatatat aattttatat
    18121 ttactcccgg attttccact tctgatgttg ttactgatat ctccgggaga ggtgttggga
    18181 tgaatgttgt tcaggaaact gttaatgagc tcaatggtag tgttgccatt gaaagtgaac
    18241 ccggtatggg cacacggttt attctgagtt tcccattaac gctggcaatt atacctgcaa
    18301 taatggtcag ggtgcaaaga gaattgtatg ccataccact ttcagatgtt atagaaacaa
    18361 tcaaaatttt gcctgaagag attacaacta tagaaggcca tgaagtaatt aatctgagag
    18421 gtgaaatttt atcacttata cgcttgaaca attttgtagg cattgaaagc ggattgaaag
    18481 gcaatgaacg tatgccagtg gttgttgttg gttatgggaa cagaaaaatt gggatagttg
    18541 tggatctatt ggaagggaag caggaaatag ttataaaatc actggaacag aattatagga
    18601 cagttgcagg cttagctggt gcttcaatat tgggtgatgg aagtatatgc ttgatacttg
    18661 atatttcttc aatgattaat aaagttataa tgcagcagga tactttttca ttcaaagaaa
    18721 aacagcgggt gttgggagca aaacaggcat cagtagaagt tgtaaaaact gaagttgcta
    18781 caaaggaaca gcctgtaaaa gaaactgtcg atgtgcaatc aaaaaaggaa attggagaaa
    18841 cgcttcttgc aaaagaaatg cctatcaaag aagatattgt atttaaaaaa gaacctgaag
    18901 aaatagttga agttgaagaa tataaagaac cagaaaaaaa ctacgaatta aaaagaggga
    18961 aagatactgt cattgaggaa aaagttcata gtgttttgga agaattcaaa acagaattaa
    19021 aagaaaaaat taaggataca ctggataaaa aagatgagat atatcagaag ttaggcatat
    19081 cagatgatga tctggcaaaa gtacaggtcc ttgcaaatgt tggcattaca gatgctgccg
    19141 aatcgctatc aaaaataatt aacagaagag tggatttgtc gatacctgat gtttcggttc
    19201 ttgctattga gtctatacca caatcttttg gtgatgttga cagtgtgtat ttaggtgtat
    19261 atatgcccat agtagggcag atcaatggaa caattttgtt cagtttacga gaagactcgg
    19321 ggtatgaact ggtggatatg cttttgggtt tgcctcaggg aacaacagat accctgaccg
    19381 aagatggtga gtcagtatta aaagaagtaa ccaatattgt agggtcagca gtggtcaatg
    19441 cctttgctga taagattgaa atggctataa aacccaccgt gccttcagtg gtgcacgatt
    19501 atatgcaatc aattctagat tctatactgg cctttcacac ggcagaaaat gaatatgcgc
    19561 ttgtgatgga tactgaattt ttccaccagg acgacagggt aattgggaag cttctgatta
    19621 tgccgacgac gtcttcgtta gctaccatag tggaaaggtt gcgaaattaa attgtatgtc
    19681 agaaaaaata aaggttctgg tagttgatga ttctgctctg gtcagaaaag taatttctga
    19741 tattcttgaa agtgataaag aaatagaagt tattggtact gcaaataatg gcaaatcggc
    19801 tgtatttaaa aatcttactt taaatcctga tgtcataacc atggatattg aaatgcccat
    19861 tatgaatggg cttgaggctt tgcaggaaat tatcaaaaca aatcccaagc ctgttatcat
    19921 gatgagtgta ttgacacaat atggtgctga agcaactttc aaggcattgg aatatggtgc
    19981 ggttgatttt atcccgaaac cttcaactgt tctggctatg agcatggatg atattgcaga
    20041 actgcttatt actaaagtta aagcagtagc aaaggcttcg cttaatttac atgtaacaaa
    20101 gagtgaacat gtttcagaaa ctaataaagt atctattatt gcaaggcatg aagataaaaa
    20161 aaagattgtt ggtattggca cttcaactgg tggtccttca gctttacaga gagtattaca
    20221 aaagttgcct aaaaatttcc cttcaccaat actcgtggta cagcacatgc ctccgggttt
    20281 tacgaaagcg tttgctgaac gattggatag tttatgcaat gtatctgtaa aagaagctga
    20341 ggatggtgaa aaagtattga acggttgtgt gtatatagct cctggcgatg ctcatattgt
    20401 ggttgaaaaa acaggtgata cccgtgttat acgcctgtca caggcagaga aagtttccgg
    20461 tcatagacct tcagcagatg tgctgtttca ttctctggca aaggaagatt cgcataatgt
    20521 tataggtgtc atcatgacag gtatgggacg tgatggtgcc agagggataa aggaaattca
    20581 tgataacgga ggttacacca ttgctcagga tgaatccacg tctgttgttt atgggatgaa
    20641 cagggttgca gttgagatgg ggggagtgga tgctgttgtg gatgtagatt caatacccga
    20701 ggagataatt tctcatattt aatgtataaa aattgaataa tgtataatcc ccacaaataa
    20761 tggtggaatt atacaatgtt caatgtgttt aggaagtgaa tatttatctg ttaaattaat
    20821 ttgcaaaatt ttgtaattaa taaaaatttt ttcttgaatg ataaagggat atcggtatta
    20881 tgtatttaag gttatgtact ggattttgga atagggatat tttatactgc ggggtaattc
    20941 tatggctaaa atactgatag ttgatgatgc taagttcatg cggacccttg tgaaggatgc
    21001 actggtgccc aaaggccatg agataatcgg agaagccgaa aatggagtag aagcagtaca
    21061 gctttataaa aagttgcggc ctgaccttgt gacaatggat attaccatgc gtgagaaaga
    21121 tggaattgaa gcagcagaag aaattttaca gtttgatcct aatgcaagaa taattatggt
    21181 gaccgcatta gggcaggaaa atttgttaac acgggctata aaaataggtg taaaggattt
    21241 tgtcgttaaa ccattccctc cggaaagact tcagaaagct gctgaaaaag cattagcata
    21301 atacaatcat taatgtttta tcgtaatatt ttttactttt ttactatttc aaaaatatta
    21361 attatcgttg cataaatata ccaattatca ttaattgtct gtttttgtac atttatatat
    21421 caaattataa caataagcgg taacactatt tgagttatga taacaccaga caacaatgat
    21481 gttcaatgta ttttacacat tgaaaatttt gacggtcccc ttgatttatt atgggatctt
    21541 ataaaaaagt caaagataga aataacccag gtttctattg ctgatattac ggagcaatat
    21601 ctggagtatt taaagctaat ggaaagcatt aatattcctg ttgccatgga atttattgta
    21661 atggccagtg aattgttata ttataaatca aaagcattgt tgccgggtgc tgatatagga
    21721 gatgaatttt ttgttccacc gcttcccccg gaacttatac aaaagcttct ggaatataaa
    21781 aaataccaga tagcatctaa aaatttattg aaattgcatg aaatgcaatc agatgtattt
    21841 acaaggcaga ttcccccttt atcttttgag gaggatgtat atattgaagt aaatcttttt
    21901 gaccttttga atgcgtttgt tgatgtactg gaagcagaaa aggaaactga gcagcaacga
    21961 atagtttttg atgaaatcct tgtgagtgac cggataatgt tactggttag tatgttgcaa
    22021 gataaggaca aaatagaatt tcacgatatg tttacgcaga atccgggaag aatggaagtg
    22081 gttgttacat tactggcttt actggaaatg acccgtatag gagctatcgc tgtgttgcag
    22141 catgcgcaat ttggcaatat atatatattt aaaaaggatt taaacaaagt ggatacattg
    22201 cattaaaaaa tccacaatca ggtaaatagt atgagtgacg atatcatgaa tgaagatatt
    22261 gaaataaaat ctgatcgcga ggacagcccg gatgatgagg caggcaatga ggaaccattt
    22321 gaaatgggcg atagtgatga tctctttggc atagtagaag ctatactgtt tgtatcagct
    22381 gaacctgtcc cattgcagtt ttttaccagg cagctgggta tagatgctac ccaggcaaag
    22441 attattattg attcgcttat agatgaatat gaagaccgtg acaaaggaat aaagattatt
    22501 gagatatctg gtgggtatca atttgtcacc aatcctaaat ttgcaagcca gttacgccag
    22561 gtaatggggt ataagaaaaa agagacattg tctaaaggga tgctggagac attagcaatc
    22621 attgcttaca aacagccaat catgcttgct gagattgaag aattgcgggg tgtgtcatca
    22681 cgaatgatgg ttgcagcact tatgcgaaaa aatcttataa agcctgttgg cagaaaagat
    22741 gttcccggaa ggccgttatt atatggcaca accgatgagt ttttgcgcta ttttggatta
    22801 aataaactat ctgacctgcc caagctatca gagataaaag aatttaattt tgaagaggaa
    22861 tgatatggat actgccagtt ttgaaaaaaa cataattgaa tcgttgtctc agttaacgga
    22921 actatacagc aaagagttgc agaacatcat gaaagaaggt gagcagggga ttttcccggt
    22981 taaagcaaaa ataatttcgc atttgcagca atatgcccgg ccaccactta cctctgagtt
    23041 aatagaaaaa attgcaacgg aaattttttc tgtggctctt tcagcagctc gccctctgaa
    23101 ggttgcatat ttaggtccag ttggaacatt tactcatatt gctctgctgg aaatttttgg
    23161 tgatgttata gaggctgtac cgcacaggac tatttcggat gtatttgatg aagttgagac
    23221 aggcagggca acatttggtg tagttccaat tgaaaacagt accgaaggtg cggtcaccta
    23281 caccatggat gaatttcttg atactgattt aaaaattgta tccgaaaaat cgctcaggat
    23341 tacttatagt ctgctatcag tatgtgacag tatcgaaaag attaaaaaaa tatattccca
    23401 tccccaatcg cttgctcagt gtaaaaactg gcttaaagca aatttgccca atgtggaaat
    23461 aaatcaggtg aattcaactg caaaagctgc tgaaactgca tcatgggata aatactcagc
    23521 cgctatcgct tcaaatattt cagctaaaat atataatctt aacatcattg ctgatggtat
    23581 tgaagattca cgtcaaaact atacacggtt tcttgtgtta gggaagcatg ataacccgcc
    23641 aacaggtaat gacaaaactt caatagtgtg tgctgtgaaa gatcagcctg gtgcactgta
    23701 taagcttcta aaaccgttca gtgaacatgg tattaatatg accatgatag agtcccgccc
    23761 tgataagaag aagatgtggg catataactt ttttattgat tttaatggac atagaactga
    23821 tcctgttgtc cagaaggcgc tggaggctat gaaacaggaa accatatttt tgaaggttct
    23881 gggatcatat caggctgaaa agcatgatta aagatttaac ataaataagg agcacggcat
    23941 tatgattata gtactaaaac ccaatgttac acaggatgaa attgatcata tagttggtaa
    24001 aatcaagtcg ctaaacttgc agccacatgt ctcaaaggga cagtacagga caattattac
    24061 ggttattggc gatgaggaca tcatccgtga acagccactt gaagcaatat caggtgttga
    24121 atcggtcaag ccaat
//
LOCUS       DAORYL010000043        24023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1747_length_24023_cov_12.655833, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000043 DAORYL010000000
VERSION     DAORYL010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 24023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24023
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1747_length_24023_cov_12.655833"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..2229
                     /locus_tag="PLV81_09005"
     CDS             25..2229
                     /locus_tag="PLV81_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOM10272.1"
                     /translation="MAGKISRREFIKIAGTGVGTLAVGGGLYKLINNVILDKNDNSPV
                     VKTPTYCEMCTYQCAGWVYKKGDVPWKIVGNDLDEHCYGRMCTKGLSGLGAYLDPNRL
                     RTPLIRKKERGKQVFKESSWDEAFTYIADKMEYIKQKYGPESVALFSHGSGGHWFKTL
                     LKAYGTNSFAAPSYANCRGPREEGYMLTYGEPIDTPERTDMKNTRCLVLIGSHIGENT
                     HSQQVIEFSQAVANGASIIVVDPRFSVAASKAKYWLPIKPGTDIALLLAWMHVLIYDE
                     LFDKRYVFNNTVGFEQLKQSVKNTTPEWAYPITSIQPELIRETAREMAKYSPATIVHP
                     GRHNVWYGDDTQRVRAGAILNALLGSWGRKGGFFYPEKVEMPEYPTPPFPHPKKSFRD
                     AVNNKYELANLIVSSGVCDATIHNVMNGNSFYEGWFIYGSNLLKALPQQEKTIKALQD
                     LELVVVVDILPTEITGWADVVLPDTTYLERYDDIRTSQGRTPQIALRMPAFNPKYNSK
                     PAWWIAKNLAEKMGLGAYFPWNTIEEYLDYKLKAVGTSLEEMKQVGVKNFERQTPLYI
                     AGSQLPRLDTPSGKIELYSSTLASYGFDPIPTYTKHEDPPKGYYRLLYGRSPFHSFAR
                     TINNPILSQLQNENTVWVHPTVAQEWGLKNSQYVKLENQDGVVSNKVRVKVTERIRPD
                     CVYMVHGFGSKQKQLKRAFKKGADDQQLMTKVNVDPVMGGQAMHGNFVTFKV"
     gene            2272..2823
                     /locus_tag="PLV81_09010"
     CDS             2272..2823
                     /locus_tag="PLV81_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOM10273.1"
                     /translation="MNSKTKKYAMVIDTRLCVGCGACVAACKMENQVPEGLHRDWIVE
                     QVTGTYPNLSLEIRSERCNHCSNAPCITSCPTGASHRKEGTNIVVVTANKCTGCKACM
                     ASCPYNARFVMPDGYVSKCTFCEHRLDEGLEPACASACPTHAITFGNLHDKMSQVSKL
                     LKVRKYKTIIPEAGTEPNIFYLL"
     gene            2852..3832
                     /gene="nrfD"
                     /locus_tag="PLV81_09015"
     CDS             2852..3832
                     /gene="nrfD"
                     /locus_tag="PLV81_09015"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HOM10274.1"
                     /translation="MNEIVLTTKKAIESGHSLGIWRWEVPIYLFLGGLTAGILIIAAC
                     MILKDKEKEFPFSTNKLILLSPILLSIGMFFLFLDLDHKLYVWRFYTAFRITSVMSWG
                     AWILILVYPLSLVLLLATVKKGYTEYYQKFSTWLKGSQYNKLSPIIEKLINWSEKYKK
                     PVAMATIPVGILLGIYTGILLSAFSARPFWNSELLGFLFLVSGLSTGAALIILLAKEH
                     HERIFITKLDIGFIVIEAIILVLYIFGMLSSSLQHIDAIQLILGGELTPVFWSFVFAI
                     GMVIPLALEILELRGKAIPVHIAASLVLVGGLALRFIFVEAGQISNWLPY"
     gene            3863..4375
                     /locus_tag="PLV81_09020"
     CDS             3863..4375
                     /locus_tag="PLV81_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOM10275.1"
                     /translation="MEKKPYASPYIAGVGLGLVLLAAFFFTGHGLGASGAITRSIVAV
                     EKVISQEHVDNNAYLAEYGGGDTNPLNNWLVFQLLGVIVGGMLSGIIAGRFRKEINHG
                     PRITPKQRWLYAIIGGALFGFGAKFARGCTSGFILTGGAGLALGAWVGMMVMFAVAYI
                     TAYFVRKLWI"
     gene            4387..4992
                     /locus_tag="PLV81_09025"
     CDS             4387..4992
                     /locus_tag="PLV81_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOM10276.1"
                     /translation="MFAPLSVYFNWSNGIDLLVSFIIGIGFGFALEQGGFGNSRKLAM
                     QFYFRDLTVLKVMFSAMVTAMTGIVFFTAFGWLDTNSMFIPQTFIWAQILGCAIMGVG
                     FAIGGYCPGTSFVGLSTLKMDALFFIIGLFIGVFLFGEMFPILEELYHGKYSGNMGRI
                     TLYEFLGVRPGIIAAIILIMAIGAFWGSEKIEEKFGNQIIQ"
     gene            5003..5623
                     /locus_tag="PLV81_09030"
     CDS             5003..5623
                     /locus_tag="PLV81_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOM10277.1"
                     /translation="MKLENKQIAVYTAILLAIIMLFLPGPDPSKVNLDLQLMAQIIGN
                     KEDHVTPGELSQWIIAGNNDYQLIDIRSKDEYDKGHIEGAINIPIENLLHKNVITDEL
                     NHDKKIVIYSNGSSHASQAWVVLKGLGLDCYILEGGYNGWNAAVLNPAVPSATTDDEI
                     LLYAKQKAVAEYFGGSNIQVQKPANNAAPVAPQKPQGVKKKKMQGC"
     gene            5750..5917
                     /locus_tag="PLV81_09035"
     CDS             5750..5917
                     /locus_tag="PLV81_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10278.1"
                     /translation="MEKDKTSGNSEKIMKDNLSRALFALQMYMNRFMWISTKEIVAGT
                     SSWYYNDTKGD"
     gene            complement(6011..6238)
                     /locus_tag="PLV81_09040"
     CDS             complement(6011..6238)
                     /locus_tag="PLV81_09040"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOM10279.1"
                     /translation="MKKNVGPADRYIRTLIGVSILLQILVLKPGAIGTIIFLALGLAV
                     LYSVYSGYCWAYDILKVSTCKESCAAEVDAE"
     gene            complement(6346..7170)
                     /locus_tag="PLV81_09045"
     CDS             complement(6346..7170)
                     /locus_tag="PLV81_09045"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOM10280.1"
                     /translation="MPTTPTYTIWVHSDIQPVYKADRWHYKRAIEDIKNIQPHPVIAI
                     LAGDIIQHKNSDEDFEWFVELRKQANIQYWYEIAGNHEWKNIQGYKKYIGKPLKYTVT
                     FGNIIFICMSNERGRPPTFLSDETFNWWKNIVIKNQDKIIITVTHAPLRNSKLLGAFS
                     KKHNIINSERFEKILKKYHVDLWISGHIHMPVWLHQNSRFAPEFGNTLFINVCGIRKD
                     PTMNVESRIFFFQEGSDKLLIRLRNHESESYKSAYDINVTLRHPFICNGCQPVMSE"
     gene            7357..8628
                     /locus_tag="PLV81_09050"
     CDS             7357..8628
                     /locus_tag="PLV81_09050"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOM10281.1"
                     /translation="MDKDNVTIDTSRFQEISISYLKKLSGDKLLTFDVLDQNGNPVFS
                     AGQALSDEVLKKKSEEGVRFFIPRVTETIGVYDREIIDMKKLHAIADDTATMYTDIRK
                     SGRMSYEQFLQSHNQLAAIIDSLRAEDKTGGVLSLLKDIKDFDYITYTHSVNVGVLAM
                     ILVYKELSTGDKVKLAALAGYLHDIGKLRVEQAIIQKPGLLSYDEFKKVLEHTREGYR
                     ILDSVVDSSGRKVVPNIIKYVALFHHRKLQSPGYPYRDDDKDKTRESQYNEIPDLARI
                     MGICDMYDALTSATPFRMPMTVEKALRYILNLSNYLYSTDDVHRFLKVLGLSLNKGNP
                     FLRIGDFIMLESEKVSLDTRKKIRVYEIARIEEISRINFLNPKVMIFYDVLRNKKLNN
                     IIVDLRYDTSRRIVRVFESTRIREMLLRQLK"
     gene            8637..9005
                     /locus_tag="PLV81_09055"
     CDS             8637..9005
                     /locus_tag="PLV81_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM10282.1"
                     /translation="MNPILIVYENDRFAQLIQFVLTTAGYQIVKSNDPLELFEKVKEL
                     KPPLIVMDVFFKHIDGLYLLERLYGIKEFAPIPVFIVSSRNEPLTLFDALERGAADYL
                     AAPINEDMLIDKVNKLLKGN"
     gene            9278..10294
                     /locus_tag="PLV81_09060"
     CDS             9278..10294
                     /locus_tag="PLV81_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOM10283.1"
                     /translation="MKKCIVVLLVLISGCSSLNGVSHTHETNLMESTQNPYQIANKLE
                     FSEVWAYLMKGEEDKIKGDEPITDLCYFSAKVSGKGQITGEYVPPSIKFSNKIRHHIV
                     IAVLNNFALMHFVLNPDLPVRKKFINDICTVASKFDGVQIDFESVASSDASWFFSFLT
                     ELRSALEKDKMLSIALPPRRTRIADAYDYPSIAAVADRVIIMAYDQHWSGSNPGPIAG
                     LSWCREVAEFAKSFIPQEKLIMGLPLYGRSWQDKNFNKSVHYHNVEEMIKASNIQPQY
                     DPENGAYFEYEEKVKIRVYYTDERAIYDRLKLYSSMSIDSVAFWRIGQGPRGMWQGIS
                     SKSL"
     gene            10310..10867
                     /locus_tag="PLV81_09065"
     CDS             10310..10867
                     /locus_tag="PLV81_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOM10284.1"
                     /translation="MKKLSYEEIKLHRPDTKELFRLQRFPVVTVLENIRSMYNVGSMF
                     RTADAARIAYMYLCGYTPYPPRKEIEKTALGSTESVPWEHSIKPVSVLRMLKQQGFTI
                     AALEHTTKSISYFSYHYTYPLCIVVGNEVEGISSELLSLCDVALEIPMFGIKHSLNVA
                     VAYGIVVFHAVMAYQSEGKISESSD"
     gene            10885..12048
                     /locus_tag="PLV81_09070"
     CDS             10885..12048
                     /locus_tag="PLV81_09070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOM10285.1"
                     /translation="MQKVILKKGKEKSVVHRHPWIFSGAIAHAEVSDGQLAAVCAHDG
                     TVLAWGYYNSRTDIAVRLLSFGDRKPDTDLFVNRICASLTLRKVTGITQCTNAYRLIH
                     SEGDMLPGLIVDWYKGHCVMQSLTLGMDKLKVTIAQIINDVLKPESIYERSDHEGRKK
                     EGLHEQSTQVYGYTPEIIEVNENGMLFLVDVRRGQKTGFFCDQRDNRKKVKEIANGRD
                     VLNLFSYTGGFSVAALLGGAKSAVSVDGSGSALQVAHRNCEMNNVGQAHEIIKADVFD
                     YINNNDITQNLIICDPPALAKNKASVNNAARGYKELNLKIISKCPADSFLLTCSCSRF
                     IDHKLFQQIVFAACNDAGRDACIVGRYSQPADHPVNIYCPETEYLKALLLYIY"
     gene            12214..13053
                     /gene="nifU"
                     /locus_tag="PLV81_09075"
     CDS             12214..13053
                     /gene="nifU"
                     /locus_tag="PLV81_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HOM10286.1"
                     /translation="MWEYTEKVKELYKNPKNVGEIPDADVVVEVGSIVCGDALTLYLK
                     LDGNKIIDAKFKTFGCGSAIASSSALTEMIKGKTIEEAAKITNKDIVEFLGGLPDQKM
                     HCSVMGQEALEKAIKKIKGHAVEDEYEDEGAIVCQCFGISENLIRRVINENNLTTVEE
                     VTNYTKAGGACGSCIPKIEDIIKEELAKGAAQKIASVQKPMSNLKRMQLVEETIQNVI
                     RPVLMRDGGDIELIEIDGKKVYVALRGHCAHCVISDVTMKNLVQEKLREFVESDIEVI
                     EVK"
     gene            13053..14264
                     /gene="nifS"
                     /locus_tag="PLV81_09080"
     CDS             13053..14264
                     /gene="nifS"
                     /locus_tag="PLV81_09080"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOM10287.1"
                     /translation="MQNKIIYLDNNATTMVAPEVVEAMMPFFMERYGNPSSMHDFGGD
                     VAHDVENARHQVALMLGADHDYEIVFTSGATESDNMAILGTLQYYRDKKHIVTTKVEH
                     PAVLNLCRQLEREGYSVTYVPVDREGNLDLDFLFDSVTNDTAIVSVMYANNETGVIFP
                     VDKIGAFCKQRGVPFHVDAVQAIGKIPVNVNAIQCDLLAISGHKFHAPKGIGVLYVRR
                     GTRMRPLLYGGHQEKARRPGTHNVPGIVGIGKASELVTKHLANTEEVNRVKRLRDRLE
                     DGLLTQCSNAHLNGAKNNRVDNTTNIGFEFIEGEAILLYLNEKGIAASSGSACSSGSL
                     EPSHVLRAMGVPFTSAHGSIRFSLSRYTTEDEIEYTLKVMPEVVNRLLEISPYWDAKT
                     KKGKPFTELAR"
     gene            complement(14293..15129)
                     /gene="flgF"
                     /locus_tag="PLV81_09085"
     CDS             complement(14293..15129)
                     /gene="flgF"
                     /locus_tag="PLV81_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HOM10288.1"
                     /translation="MVRGLYTGASGMIAQEARLDAIANNLANVDKTAYKKDLTLFKAF
                     PDMIIRRLNDDGLGITPAGSYDTMPFVGKLGTGVEVNEVYTIYDQGSLMRTENPFDMA
                     LDGKGFFTVMTERGERYTRNGAFTLNQDGILVTHNGLPVLGQNGIIKLQKNNFMINER
                     GEILVNAALSLEPTDLVGMTQNNWEQPVVIDKLKIVDFENIRELKKEGESLYRETEYS
                     GPALPAEEYKVVQGFLEKSNVNAVREMVDMIEVQRSYEANQKAMLTHDQELGRLINEV
                     AR"
     gene            complement(15129..16397)
                     /gene="murA"
                     /locus_tag="PLV81_09090"
     CDS             complement(15129..16397)
                     /gene="murA"
                     /locus_tag="PLV81_09090"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOM10289.1"
                     /translation="MDTYIINGGKKLNGSVTISGAKNAALPIIVASLLAEGKTVLHNV
                     PNLKDIQTILQVIKCLGADYQFDTQTNTLTIHVNSLKNAEVPYDLVKTMRASIYVMGP
                     LLARCGEADVSLPGGCAIGERPVDIHLSGFEALGSTINIEHGYIKARAKKLIGNRFIM
                     RKVSVGATANIMMAAVLADGETILENCAMEPDVVDLGNFLIKMGAKIDGLGTERIVIK
                     GVKKLKPVTYSIIPDRIEAGTFLIAGAITRSNITINKVIPEHFQACIDILSEMGFVIA
                     GGDNSITIEPSKKLQGSMIRTLPYPGFPTDLQAPIMSLMCTIPGISVIIETIFENRYT
                     HVGELRRMGADIEIEGNVAVVKGGNQLQAAPVMMSDLRAGASLVLAALAAKGQTEIRR
                     IYHTDRGYENFEQKLQSLGADIQRVKGGKP"
     gene            16542..17927
                     /locus_tag="PLV81_09095"
     CDS             16542..17927
                     /locus_tag="PLV81_09095"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOM10290.1"
                     /translation="MTKKYELSLHIFRRDLRLDDNTALIRACDLSDYVIPCFILDDRQ
                     LYNNPYRGDNAVQFMIQSLRDLNNELVKKGSRLYLFKGIAEDVVEKLIKEENINAVFF
                     NRDYTPFSTMRDGKIREICKRYNRDCNIYADALMNEPEMVVTQDGRPYTIYSHYLKKA
                     RMFAVSSPLKNKFNNYYTKNIKSEEPHSILDTIIVNENPHLFLKGGRKEALNLLLKIA
                     HLQHYDETRNIPAVQGTSLLSAHIKFGTVSIREVYHTAITHLKSPDSFINELYWRDFF
                     TSIAFHFPHVFKGAFHKQYDALQWANDKEKFELWTNGLTGFPIVDAGMRQLNTTGWMH
                     NRVRMIVASFLVKDLHIDWRWGERYFATKLIDYDPSVNNGNWQWAASTGCDAQPYFRI
                     FNPWLQQKKYDPECEYIKRWIPELQIVSLKDIHNWVYSGSSVKYPKPILDHVIESSYA
                     KGIYKSIFRNE"
     gene            18083..19243
                     /locus_tag="PLV81_09100"
     CDS             18083..19243
                     /locus_tag="PLV81_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOM10291.1"
                     /translation="MVIGILKEIMEGEKRVAATPQTVKKMIADGYKVLVETGAGEGSF
                     YNDDEYKAVGATITDTESIYHESEVILKVKEPLFNNILNIHEVDLLHSGQIIISFLHP
                     ASPDNHEMIKKLTAKGVTGLTLDGVPRISRAQSMDALSSMSSCAGYKGMLLAANAIAK
                     FMPMITSAVGVVKPANVLVVGAGVAGLRGIATAKGLGAVVYAADIRPDANEQAKSLGA
                     KIIDTGVPADIAVSQDGKYANKLPEEWLKVERESLKDIIAQADIVFLTALVFGKKAPI
                     LVDEEMVKLMQPGSCIADISIDQGGNCSITQPGVISQKHGVNIIGYKNIPGMLPASST
                     DMFSNNIYNLLKYLTVDGKIKLDLSDEIVKSILVCHEGVLLHEGTLEAMSQK"
     gene            19272..19580
                     /locus_tag="PLV81_09105"
     CDS             19272..19580
                     /locus_tag="PLV81_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOM10292.1"
                     /translation="MTALQMILLLIFVFTTFIGYRLIKNVPSLLHTPLMSGMNALSGV
                     TILGALIATSLSLANGNAIVSWLMGGIAIILAMINVCGGFAVTDRMLKMFSKKDNRNE
                     "
     gene            19573..21012
                     /locus_tag="PLV81_09110"
     CDS             19573..21012
                     /locus_tag="PLV81_09110"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOM10293.1"
                     /translation="MNDTLLSCLSVTSINNAYYIFAFFITIMVLFGIFLMSKIRYARY
                     GNLLSAIAIALAIIVTLIKNQIITTGSLYIFVVIGAVIGLRVAYKVKMIQMPQLVALL
                     NGSGALASAIVGAYSYFGIGAGLDMFTRVTAIIAFQVGIITFFGSLIAAGKLHQVIPQ
                     APVILPYHTHFSIIIFASLICIVICSVNNIIADSLFLIVSFIFSLIFGVFFTIRIGGA
                     DMPITISLLNSMSGIAGAICGLAIGDFLLVSIGGIVGASGLLLTQIMCWAMNRNLLDI
                     LFGKTTIAAKKRSDNDIIKGDQSKGVYKSQANPLEVIKNAKDVIIVPGYGMAISQAQH
                     LVKQLSDILIANGAKVRFAIHPVAGRMPGHMNVLLAEANVDYDDLYEIDNINPEFKNA
                     DLAIIVGANDVVNPAAREAKDTPIYGMPILNVDECKNIFVFNYDLKPGYAGVDNPLYI
                     RNEGIYFFLGDAAEQLKKFIDDFLLSKVN"
     gene            complement(21032..21601)
                     /locus_tag="PLV81_09115"
     CDS             complement(21032..21601)
                     /locus_tag="PLV81_09115"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOM10294.1"
                     /translation="MIARIDHVSLAVPDFDKAKIFFENLGAVAGVGAKDDSTKFYWQI
                     FSLGDMSRIELITPTGKGSFLDGFIESRGSGVHHITLQTHDIKKAISKLEQMGVPYFG
                     YHEYPGGVWKEIFIHPKHAFGVLIQIAEFNADDWLSPEVKMPDGQYKIEVNEGKILLT
                     LPHPGGGTATIQLTKEQAKHLAQSLLSKI"
     gene            21818..22309
                     /locus_tag="PLV81_09120"
     CDS             21818..22309
                     /locus_tag="PLV81_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10295.1"
                     /translation="MHIETTTCISLTNLSILENYAKQLNVPLRSLIMYLLMYAAKNEK
                     RKAIAFKRLSYRKRNTEDSWRRVHLVLYHSEYEFILDVKKLWKMSLARCIEFCIDNIL
                     FEFINYYMDQLRRKDTDNYLPYYQNRSYTLDFYQEKGIHCITCYWGPPPELLNNTLSL
                     ELA"
     gene            22479..>24023
                     /locus_tag="PLV81_09125"
     CDS             22479..>24023
                     /locus_tag="PLV81_09125"
                     /inference="COORDINATES: protein motif:HMM:NF020022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOM10296.1"
                     /translation="MQQIVSFLSNPKSYNHPVDTVTVVQTHISYVFIVKPYVYKLKKP
                     VDFGFLNFISFEDRKFFTFEEYRLNSRISPEIYLGVVAIVKDGNSFRLVEDAAYNEND
                     VVAYCVKMKYIDDEFSLKNLLKQGISNQLLSLIAQKIYAFHKNADNTEGLQGFGGINE
                     ISKNTLENFDQIEKYIGIAISKNDYTFMRSWTEDFINTHQEFFKDRNIQGFVRDCHGD
                     LHCEHIYYKDDSIIILDCIEFNKRFRYIDTISDIAFLLMDLDVNGNIGDSNRLCAQYI
                     QLSGDYDGVLVLPFYKAYRAMVRAKINSFQSDNEGITQHERDAYIQKASQYFQLAKHY
                     MQQNAKGVVYIVFGNIGSGKSTISMEMASLTGGIVINSDAVRKKLAGLDVFDKANSKA
                     GKGIYTPEMTEKVYATMKQKAMAIALAGMPVILDGTFYSKELRRNFYQFFKEKEIDVQ
                     FVYTQCPEEELIRRIERREKDTSISDADVEIFMQLKDRFEKPEDDVPELYVVNSNTSI
                     EDIRNQL"
BASE COUNT         7627 a         3898 c         4928 g         7570 t
ORIGIN      
        1 ctatcttctg caggagaatg tataatggca ggtaaaatca gtagaagaga atttatcaag
       61 attgctggga caggtgttgg tactctggcg gttggtggag gcctttataa attaataaat
      121 aatgtaattc tggacaaaaa tgacaatagt ccagtagtca aaacacctac atattgtgaa
      181 atgtgcacct atcaatgtgc aggatgggtt tataaaaagg gtgatgtgcc ctggaagatt
      241 gttgggaatg accttgatga acattgttat ggccgaatgt gcacaaaagg gttatccggg
      301 cttggagcat atttagatcc caatagattg agaacaccct taataaggaa aaaggaaaga
      361 ggaaagcagg tatttaaaga atcttcatgg gatgaggcat ttacttatat tgctgacaaa
      421 atggaatata ttaagcaaaa gtatggccct gaaagtgttg ccttattcag tcatggttca
      481 gggggacatt ggtttaagac attactgaaa gcgtatggta ctaatagttt tgcggcacct
      541 tcctatgcta attgccgtgg ccctcgtgaa gaaggttata tgcttaccta tggagaacca
      601 attgatactc cagaacgtac tgatatgaaa aataccagat gtcttgtgct tattggttct
      661 catattggtg aaaatacaca cagtcagcag gttatagaat tttctcaggc agttgccaat
      721 ggagcaagta tcatagtagt agatccacgt ttttcagtgg ctgccagcaa ggccaaatat
      781 tggttgccaa ttaaaccggg tactgatatt gctttgcttt tagcatggat gcatgtattg
      841 atttatgatg aattatttga taaacgatat gtatttaata atactgttgg atttgagcag
      901 ttaaagcaat cagtaaaaaa tacaacacct gaatgggcat accccattac atcaattcag
      961 cccgaactca tacgtgaaac agccagggaa atggcaaagt attctccggc tacgattgtt
     1021 catcctggaa ggcataatgt atggtatggt gatgatactc aaagagtaag agcaggcgca
     1081 attttaaatg cattattagg tagctggggt agaaaaggtg gattcttcta tcctgaaaaa
     1141 gttgaaatgc ctgaatatcc aactccacca ttccctcatc caaaaaaatc atttagagat
     1201 gcagtgaata ataaatatga attagcaaat ttaattgttt catcgggagt ttgcgatgcc
     1261 actattcata atgtcatgaa tggtaattca ttttatgaag ggtggtttat ttatggctca
     1321 aatctattga aagctctccc tcaacaggaa aaaactatca aagccttaca ggatcttgaa
     1381 ctggtggtcg ttgtagatat tttaccaaca gaaataacag ggtgggcaga tgttgttctc
     1441 cctgatacta catatctcga gcggtatgat gatataagaa cttcacaagg gcgaacccct
     1501 caaatagctt tacgaatgcc tgcattcaat cccaaatata attccaaacc tgcctggtgg
     1561 atagccaaaa accttgcaga aaaaatgggt cttggagctt atttcccctg gaatactatt
     1621 gaagaatatt tggattataa attaaaagca gtcggtacat ctcttgaaga gatgaaacag
     1681 gttggggtta agaactttga aagacaaact ccactatata ttgcaggctc tcagttgcca
     1741 cgattggata cccccagtgg aaagattgaa ttgtattcat ctacattagc aagttatggt
     1801 tttgatccta tacctaccta tacaaaacat gaagatcctc caaaaggtta ttatagatta
     1861 ttgtatggca ggtcaccttt ccattcattt gcgcgtacta taaataaccc tattctgtca
     1921 caattacaga atgaaaatac tgtttgggta catccaacag ttgcacagga gtggggtttg
     1981 aaaaacagcc aatatgtaaa actggaaaat caggatggtg tggtgagcaa taaggttaga
     2041 gtcaaggtaa cagaacgaat acggccagat tgtgtgtata tggttcatgg atttggaagt
     2101 aaacaaaagc aattaaagcg tgcatttaag aaaggcgctg atgatcagca acttatgact
     2161 aaagttaatg ttgatccagt tatgggtgga caagctatgc atggtaattt tgttaccttt
     2221 aaagtttagt taattattga accatatttc agataatgaa aagaggtcag catgaatagt
     2281 aagactaaaa agtatgcaat ggtgatagat acccgcttat gtgttggttg tggagcatgc
     2341 gttgcagcat gtaagatgga aaatcaggtt cctgaaggct tgcaccggga ttggattgta
     2401 gagcaggtta cagggactta tcctaatctt tcattggaga tccgttctga gcggtgtaat
     2461 cattgttcca acgccccctg cattacatca tgcccaactg gagcaagcca tcgtaaagag
     2521 ggtacaaata ttgttgttgt tacagccaat aaatgcacgg gttgtaaagc atgtatggct
     2581 tcttgcccat acaatgcccg ttttgtaatg cctgatggtt atgtcagcaa atgtactttc
     2641 tgtgaacaca gattggatga aggtttggaa cctgcctgtg cgtctgcatg ccccacacat
     2701 gcaataacat ttggcaactt acatgacaaa atgagtcagg tttccaaatt attgaaagtg
     2761 agaaaatata aaacaattat cccagaagct gggactgaac caaatatatt ttatttactg
     2821 tgaaattaaa gaaattatat gaggtttgac tatgaatgaa atagtattaa ctacaaaaaa
     2881 agcaattgaa tcaggccatt cattgggaat atggagatgg gaagtgccta tatatctttt
     2941 ccttggtgga cttactgcag gaattcttat tattgcagca tgtatgattt taaaagataa
     3001 agaaaaggaa tttccttttt ccaccaataa attgattcta ctgtcaccaa ttcttttaag
     3061 tattggcatg tttttcctat ttctggatct tgaccataaa ttgtatgtat ggcggtttta
     3121 cacagctttt cgcattacat cagtaatgtc gtggggtgca tggatattaa tcctcgttta
     3181 tcccttaagt ttggtgttac tattagcaac tgttaaaaag ggttatactg aatattatca
     3241 aaagttttct acgtggctga aaggatctca atataataaa ctatcgccca ttattgaaaa
     3301 acttattaat tggtcagaga agtataaaaa gccagtagca atggcaacaa tacctgttgg
     3361 aatattattg gggatatata ccgggatact tttaagtgca ttttcagcac gaccattctg
     3421 gaattccgaa cttcttggat ttttattttt agtttcaggc ttatcaacgg gtgctgcatt
     3481 aattatttta ttagcaaaag agcatcatga gaggatattt ataacaaaac tggatatagg
     3541 ttttattgtt atagaagcta taattctggt tctgtatatt tttggaatgc tttcttcatc
     3601 attgcaacat attgatgcta tacaattaat tttaggcgga gagctgactc cagtattctg
     3661 gtcttttgta tttgctattg ggatggttat ccctctggca cttgagatac ttgaactcag
     3721 gggcaaagca atccctgtgc atatagctgc ttctttggta cttgttgggg gattggcatt
     3781 gcgttttatc tttgttgagg ctggtcagat ttctaactgg cttccgtatt aaaatatcat
     3841 ttattttcag gaggaatatt gtatggaaaa gaaaccttac gcatcaccct acatagctgg
     3901 agtgggttta gggttggtat tattggcagc tttcttcttt acaggccatg ggcttggagc
     3961 ttcgggagca attactcgtt ctattgtagc tgttgaaaaa gtcatttcac aggagcatgt
     4021 tgataataat gcttatcttg ctgaatatgg tgggggtgat acaaacccat tgaataactg
     4081 gcttgttttt caattgttgg gcgttattgt cggtggtatg ttatcaggaa ttattgcagg
     4141 aagatttaga aaggaaatta atcatgggcc tcggattaca ccaaaacaac gatggcttta
     4201 tgcaataatt ggtggtgccc tttttggatt tggagcaaaa tttgcacgcg gttgcaccag
     4261 tggttttata ttaacaggtg gagctggact tgcattgggt gcatgggtgg gaatgatggt
     4321 gatgtttgct gttgcatata tcacagcata ctttgttaga aaactttgga tataaggggt
     4381 gagtatatgt ttgcaccatt atcagtatat tttaattgga gtaatggaat tgatttactg
     4441 gtttcattta taataggtat tggatttgga tttgcactag aacagggtgg atttggaaat
     4501 tcaagaaaat tagctatgca gttttacttc cgtgacctaa cagttttgaa agtaatgttt
     4561 tcagcaatgg ttacagcaat gacagggata gtgttcttta ctgcatttgg atggcttgat
     4621 accaatagta tgtttattcc acagacattc atatgggctc aaatattggg ctgtgctatc
     4681 atgggtgttg gttttgctat tggaggttat tgtccaggca ccagttttgt gggtctatct
     4741 acattaaaaa tggatgctct tttcttcata ataggccttt ttataggggt atttttgttt
     4801 ggtgaaatgt ttccaattct tgaagaactt tatcatggta aatacagcgg taatatggga
     4861 aggataacat tatatgaatt tttgggtgta cggcctggta tcattgcagc tattatttta
     4921 attatggcaa ttggtgcatt ctggggatct gaaaagatag aagaaaaatt tggtaaccaa
     4981 attatacagt aaggagtgta ctatgaaact tgaaaacaaa caaatagctg tatacacggc
     5041 tattctatta gcgataatta tgctgtttct tcccggtcct gatccatcta aggttaattt
     5101 agatttacag ctaatggcac aaattatagg aaataaggaa gatcatgtta cacctggaga
     5161 attgtcgcaa tggataatag cagggaataa tgactatcaa ttgattgata tacgatcaaa
     5221 ggatgaatat gacaaaggac atattgaagg agcaattaac atcccaattg aaaatctttt
     5281 acataagaat gttataactg atgagcttaa tcatgacaaa aaaattgtga tatattcaaa
     5341 tggaagctca catgcatcac aagcatgggt tgtgttgaaa ggattggggc ttgactgtta
     5401 tattcttgaa ggtggatata atggttggaa tgctgctgta ctcaatccag cagtaccatc
     5461 agctactaca gatgatgaaa tattgcttta tgccaaacaa aaagctgttg cagaatattt
     5521 tggtgggagc aatattcagg tacaaaaacc agctaataat gctgcacccg tggcaccgca
     5581 aaaaccacag ggagtaaaga agaaaaagat gcaggggtgt taaataagaa aataatttat
     5641 ttttatataa aaggggctat aataatttgc ccctttttta tttgattttg tattgtaata
     5701 aagttattta tgatgtatag gtaatttaaa aataataaaa gaatgtaata tggaaaaaga
     5761 taaaacatca ggtaacagtg aaaaaataat gaaggacaat ctttcaaggg cactttttgc
     5821 acttcaaatg tacatgaatc gcttcatgtg gatatcaaca aaagagatag ttgctggaac
     5881 aagttcatgg tattataatg acacaaaagg tgattaggta aattcctaag tagtaaaatt
     5941 attgaaattg gaataaaaaa aagcaggatg ttctcctgcc ttttttacga ttatgatacc
     6001 ttaaagggta ttattcagca tcaacctcgg ctgcacatga ttccttgcat gtgcttacct
     6061 ttaaaatatc atatgcccag cagtaaccac tgtatacaga atacaatact gcaagaccca
     6121 atgctaaaaa tattattgtc ccaatagctc ctggttttaa aacaaggatc tgaagtaaaa
     6181 tagaaacccc aattaaagtt ctgatatacc tgtcagctgg tccaacattc tttttcatat
     6241 atcacctctg aatagcaaaa ttaaaatata aagaattatc tcaaagtata tgtttttata
     6301 caataaaaat atggcaagta ttttttgcta tttagtattc aaaatttatt ctgacataac
     6361 aggctgacat ccattacaga taaaaggatg gcggagggta acattaatat cataggcgga
     6421 tttatatgat tcggattcat ggttacgtaa tctgattaaa agtttatcag aaccttcctg
     6481 gaaaaaaaat atccttgatt ccacattcat ggttggatct ttacgtattc cgcatacatt
     6541 aataaaaagg gtatttccaa attctggtgc aaatctactg ttttgatgta accatactgg
     6601 catatgaata tggccgctta tccataggtc aacatggtac tttttcagta tcttttcaaa
     6661 gcgttctgag ttgattatat tatgtttttt agaaaaagct cccagtaatt tactgttgcg
     6721 caatggtgca tgagtaactg tgataataat cttatcctga tttttaatta caatgttttt
     6781 ccaccagtta aatgtttcat ctgaaagaaa tgttggcggt ctgccgcgtt cattggacat
     6841 gcatataaaa attatattcc caaatgtaac cgtgtatttt aaaggtttac cgatatattt
     6901 tttataacct tgtatatttt tccattcatg gttacctgca atttcatacc agtattgaat
     6961 atttgcttgt ttacgtaatt caacaaacca ttcaaaatcc tcatctgaat ttttgtgttg
     7021 tattatatct ccggcaagaa ttgcaatgac aggatggggc tgtatgtttt ttatatcctc
     7081 aatagctctt ttataatgcc atctgtcggc tttatataca ggttgtatgt cggagtgaac
     7141 ccatatagta taggtgggcg tggttggcat gtattgcttg cagcatcctg gcactataag
     7201 caatagtata attactatac tggggtattt tctcatggca ttatttactg taaactatcg
     7261 cacaaatact tttgaattta aaaaaaatat tgcaacaaat acaatattgt atcataatta
     7321 acattaaatc aagttaattt taatatcaga agaattatgg acaaagataa tgtaactatt
     7381 gatacatccc gctttcaaga gatatcaata tcgtatttaa aaaaactcag tggcgataag
     7441 ttgcttacct ttgatgtact ggaccagaat ggcaatcctg ttttctcagc tggccaggca
     7501 cttagtgatg aagttttaaa aaagaaatcc gaagagggtg tgcggttttt catcccccgt
     7561 gttactgaaa caattggtgt ctatgaccgc gagatcattg atatgaagaa gctgcatgcc
     7621 attgctgatg atactgcaac aatgtataca gatatccgta aatcagggcg gatgtcgtat
     7681 gagcagtttt tgcaatcaca taatcaattg gcagctatta ttgatagcct gagggctgag
     7741 gataagacag gaggtgtgtt atcattgctg aaggatatca aggattttga ttatatcacc
     7801 tatacgcatt cggtcaatgt tggtgtactg gcgatgatat tggtatataa ggagctttca
     7861 acaggagaca aagttaaatt agctgctctg gcgggttatt tacatgatat tgggaaattg
     7921 cgtgttgaac aggccatcat tcaaaagccg ggattgcttt catatgatga atttaaaaaa
     7981 gtactggaac atacccgtga agggtatcgc atccttgata gtgtagtgga ttcatcagga
     8041 cggaaagtag tacctaatat tataaaatat gtggcactat tccatcatcg caagcttcaa
     8101 agccccgggt atccttaccg tgatgatgat aaggacaaaa cacgtgaatc gcagtataat
     8161 gagatacccg accttgcacg tattatgggt atttgtgata tgtatgatgc ccttaccagt
     8221 gcaacaccgt tccgtatgcc aatgacagta gaaaaggcat tgcgctatat attaaatctt
     8281 tcaaattacc tctattcaac tgatgatgtt catcgcttcc ttaaggtttt agggctatcg
     8341 ctcaataaag gaaatccgtt tttaagaatt ggcgatttta tcatgcttga atctgaaaag
     8401 gtaagccttg atacccgtaa aaagatacgt gtctatgaaa tagcccgtat tgaagagatt
     8461 tcacgtatta attttttgaa tcccaaagta atgatttttt atgatgtatt acgaaataaa
     8521 aagcttaata atataattgt agatttgcga tatgatacaa gtcgcaggat tgttcgagtt
     8581 tttgaaagta cacgaatccg ggaaatgctg ttacggcagc tcaaataggt gaacgtatga
     8641 atccaatatt gattgtatat gaaaatgaca gatttgctca gcttattcag tttgtactaa
     8701 caactgccgg ctaccagatt gttaagagta atgacccgtt ggaattattt gaaaaagtca
     8761 aagagttaaa acctccactt atcgttatgg atgtgttttt taaacatatt gatggattat
     8821 atttattaga acggttgtac ggaattaagg agtttgcacc tattcctgtc ttcattgttt
     8881 cttcccgtaa tgaaccactc acactttttg atgctcttga gcgtggtgca gctgattatt
     8941 tagctgcacc cataaatgaa gatatgctga ttgataaagt gaataaatta ttgaaaggaa
     9001 actaaaacaa acactgttgt tttgaaataa ttccatccga caaatatagt agcgtctcat
     9061 atcaattaat gaggaaattt catatttgtg agcagtttct gaatattggg acgatgaagt
     9121 aatctaatgt tttaaagttg cgattgcagg tttcgattgc atcagggtca cagtcacgtt
     9181 gccaggaatg actattcatt tgaaaaatct ttgctatacc aaaattgaga ttggcatgac
     9241 tttattgaaa attttattat tttaggaaga atcggcaatg aagaaatgta ttgttgtgct
     9301 tttggtgctt atatcaggat gttcctcatt aaatggtgta tcacatacac atgaaacaaa
     9361 ccttatggaa tctacccaaa acccatacca gatagctaat aaattggagt ttagtgaagt
     9421 gtgggcatac ctcatgaaag gtgaagaaga taaaatcaaa ggtgatgagc caattactga
     9481 cctttgttat ttcagtgcaa aagtaagtgg caaagggcag ataaccggcg aatatgtacc
     9541 accttcaata aagtttagca ataaaatacg gcatcatatc gttatagctg ttcttaataa
     9601 ttttgcactt atgcattttg tacttaatcc tgatttgcct gttcgcaaaa agtttattaa
     9661 tgacatatgc acagtagctt caaaatttga tggtgtgcag atagattttg aatcggttgc
     9721 ttcatcggat gcttcatggt tttttagctt tttaaccgaa ttgcgttctg ctctggaaaa
     9781 agataaaatg ctttcaattg ccctcccacc acgcagaaca agaatagccg atgcttatga
     9841 ttacccttcc attgcagcag tagctgatag agtcatcatt atggcttatg accagcactg
     9901 gtcaggaagt aaccctggtc caatagctgg attatcatgg tgcagggaag tagctgaatt
     9961 tgcaaaaagt tttataccgc aggagaagct aatcatgggg ctcccacttt atggaagatc
    10021 gtggcaggat aaaaatttca acaagtcggt acattatcat aatgttgaag aaatgataaa
    10081 agcttccaat atacaaccac agtatgatcc tgaaaatgga gcttactttg agtatgaaga
    10141 aaaggtaaaa atacgtgtgt attatacaga tgaaagggca atatatgatc gtcttaaact
    10201 gtattcatca atgtcaattg acagtgttgc tttctggcga atagggcagg gcccaagggg
    10261 gatgtggcag ggaatcagtt caaaaagcct gtagtgattc tgttaaaaaa tgaaaaaatt
    10321 atcctacgaa gaaataaaac tacatcgccc tgatacaaag gagttattca ggttacaacg
    10381 tttcccggtt gttacagtac tggaaaatat acgtagcatg tataatgttg gttcaatgtt
    10441 cagaaccgct gatgcagcac gcattgcata tatgtatctg tgcgggtata ctccgtatcc
    10501 gccacgaaaa gaaattgaaa aaacagcctt aggcagtacc gaaagtgtgc cttgggaaca
    10561 ctcaattaag cctgtatctg tgttacggat gttaaaacag caggggttta ccattgctgc
    10621 actggagcat accacaaaaa gcatctctta cttttcatat cattacacct atccgctgtg
    10681 tattgtggtt ggcaatgaag tggaaggtat ctcaagtgaa ctgttgtcgc tctgtgatgt
    10741 ggcactggag atccctatgt ttggtataaa gcattcactt aatgttgcag tggcgtatgg
    10801 tatagtggta tttcatgcag ttatggcgta tcaatcagaa gggaagattt cagaatcatc
    10861 tgattaatgt gtacatacaa cataatgcaa aaggtaatct taaaaaaagg aaaagaaaaa
    10921 tcagtggtgc ataggcaccc gtggattttt tcgggggcga tagctcatgc agaagtgtca
    10981 gatggacagt tagcagcagt gtgtgcacat gatggcaccg tgcttgcgtg gggatactac
    11041 aacagccgca ctgatattgc tgtacggtta ctttcatttg gtgatcgcaa gccggatact
    11101 gacctttttg tcaatcggat atgtgcttcg cttacattac gtaaggtaac gggaattacg
    11161 caatgcacca atgcatatcg cctcatacat tcagaaggcg atatgctgcc gggattaatt
    11221 gttgattggt ataaggggca ttgcgtcatg cagagtttaa cattaggcat ggataaatta
    11281 aaagtaacta tcgctcaaat tattaatgat gtactaaaac cggaaagcat ttatgaacgc
    11341 agtgaccacg aaggaagaaa aaaagaaggg ttacatgaac aaagtacgca ggtgtacggg
    11401 tacacaccgg aaataattga ggtaaatgaa aatggcatgc tatttttggt tgatgtacga
    11461 cggggacaaa aaacaggatt cttttgcgat cagcgtgata acaggaaaaa agttaaggaa
    11521 attgcaaatg ggcgggatgt gctgaatctt ttttcgtata ccggtggatt ttcggtagct
    11581 gctcttttgg gtggtgctaa aagcgcagtt agcgttgatg gttctggctc tgcgttacag
    11641 gttgcacatc gtaactgcga gatgaataac gtggggcagg cacatgaaat tattaaagct
    11701 gacgtatttg attatattaa taacaacgat attacgcaga atctgattat ttgcgatccc
    11761 ccggcattgg ctaaaaataa agcatcggta aataatgccg cgcggggata taaagaatta
    11821 aatttaaaaa ttatttcaaa gtgcccggct gattcgtttt tgcttacctg ttcatgttcg
    11881 cggtttattg accataagct ttttcagcag attgtgtttg ctgcctgcaa tgatgccgga
    11941 agggatgcat gcattgtagg gcgatactca caacccgccg atcacccggt gaatatatat
    12001 tgccccgaaa ctgaatattt aaaggcgctg cttttgtata tttattgaga atttcaggga
    12061 ataaaaaata caaaaaataa ttgacgtaaa tgttaaatac tactataatg gtagtatata
    12121 atatgatatg tattattggt ataaaaatat attgattttt atctggtatg aaggctttat
    12181 tcatcaatag tacatatcat aagggagagt tttatgtggg aatatactga aaaagtaaaa
    12241 gaattatata aaaatccaaa aaatgtgggt gagattcctg acgcagatgt ggtagttgag
    12301 gttggtagta ttgtatgtgg tgatgctctg acactttatc ttaagcttga cggcaataaa
    12361 attattgatg ccaagtttaa aacctttggt tgtggaagtg ctattgcttc gtcatcagct
    12421 ttaacagaaa tgattaaagg taaaacaatt gaagaggctg caaagattac caataaagat
    12481 attgttgagt ttttaggtgg attgcccgat caaaaaatgc actgctcggt aatggggcag
    12541 gaggcgcttg aaaaagctat aaagaaaatc aaagggcatg cagttgaaga tgagtatgag
    12601 gatgaaggtg ctattgtgtg ccagtgtttt gggatttctg aaaatttgat acgacgggtt
    12661 attaatgaaa acaatcttac tactgtggaa gaggttacta actataccaa ggcaggtggt
    12721 gcttgcggat cgtgtattcc aaaaattgag gacattatta aagaggaatt ggcaaaaggt
    12781 gctgcgcaaa agattgcttc agttcaaaaa ccaatgtcca atttaaagag gatgcagctt
    12841 gttgaagaaa ccattcaaaa tgtcattcgc ccggttttaa tgcgtgatgg tggtgatatt
    12901 gaacttattg aaatcgatgg aaagaaagtg tatgtagcgc tccgtggcca ttgtgctcac
    12961 tgcgttattt ctgatgtaac catgaagaat ctggttcagg agaagttacg tgagtttgtt
    13021 gaaagcgata ttgaagtaat tgaggtaaag taatgcaaaa taaaattata tatcttgata
    13081 ataatgcaac aacaatggtt gctcccgaag ttgtagaagc aatgatgcct ttttttatgg
    13141 aacgatacgg caacccttcc agcatgcatg attttggtgg tgacgttgcc catgatgttg
    13201 agaatgcccg ccatcaggtt gcactgatgt tgggtgctga tcatgattat gaaattgtat
    13261 ttaccagtgg agcaaccgaa agcgataaca tggcaatact ggggacattg caatattaca
    13321 gggacaaaaa gcacattgtg acaactaaag tagaacatcc tgcggtactt aatctctgca
    13381 ggcaacttga gcgtgaaggc tattcggtta cctatgttcc cgttgatagg gaaggtaatc
    13441 ttgatttaga ctttcttttt gattcagtga caaacgatac agcaattgtt tcggtaatgt
    13501 atgccaacaa cgaaactggt gtaatttttc cagtcgataa gataggtgca ttttgtaaac
    13561 agcgcggtgt tcctttccat gttgatgcgg tacaggcaat aggaaaaatt ccggtcaatg
    13621 tcaatgctat acagtgtgat ttactagcca tctcaggcca taaatttcat gcacccaaag
    13681 ggattggcgt gttatatgtc cgcagaggaa cccgtatgag gcccttgtta tatggaggac
    13741 atcaggaaaa ggcacgaaga ccgggaaccc acaatgtacc gggtatagta ggcattggta
    13801 aagcttctga attagtgaca aagcatcttg caaacactga ggaagtaaat cgtgtaaaac
    13861 gtcttcgtga caggctggaa gatggtttac ttacacagtg cagcaatgct catcttaatg
    13921 gagcaaaaaa caatagggtg gataatacta caaacatagg atttgaattt attgaaggtg
    13981 aagcaatatt actatatctc aacgaaaaag gcatagcagc ttcatcaggt tctgcgtgtt
    14041 cttcaggctc gctggagcca tcacatgtat tgcgtgcaat gggtgtacct ttcacttcag
    14101 cacatggctc tatacggttt agtttaagcc ggtacacaac tgaagatgaa attgagtata
    14161 ctttaaaagt aatgcctgag gttgtaaaca gattgctaga aatttcacca tattgggatg
    14221 caaaaaccaa aaaaggcaag ccctttactg agcttgcccg ttaattattg ctattttcaa
    14281 gataaaattg atttagcgtg ccacttcgtt gattaaacgt cctaattcct gatcgtgggt
    14341 gagcattgct ttctggttag cttcgtaact acgctgtact tcaatcatat ccaccatttc
    14401 acgtacggca ttgacattgg atttttccag aaaaccctgt actactttat attcttcagc
    14461 aggaagtgcc ggacctgaat attctgtttc tctgtataat gattcgcctt ctttttttaa
    14521 ttcacgaatg ttttcaaaat caactatctt tagcttatca attaccacag gctgttccca
    14581 gttattctgt gtcatgccta caagatcagt gggttcaagc gataatgctg cattaacaag
    14641 tatttcccca cgttcgttta tcatgaaatt gtttttttgc agtttgatta tgccattctg
    14701 tccaagaacc ggaaggccat tatgggttac cagtataccg tcttgattaa gtgtaaaagc
    14761 gccattgcgt gtatagcgct cgccacgttc ggtcatcact gtaaaaaacc ctttgccatc
    14821 aagtgccata tcaaacggat tttcagttct cattaacgaa ccctgatcat aaatggtata
    14881 cacttcgttg acttcaacgc cggtacctaa tttgccaaca aatggcatag tatcgtaact
    14941 ccctgcaggg gtaataccca atccatcatc attcagacgg cgaattatca tatcagggaa
    15001 ggctttaaat aaagtaaggt ctttcttata tgcggttttg tcaacgttag ccagattgtt
    15061 ggcaattgca tccaggcgtg cttcctgagc aatcatgcca cttgcgccgg tatacagtcc
    15121 tcgtaccatt atggttttcc tccttttact ctttgtatat cggcacctaa ggattgtaac
    15181 ttttgctcaa aattttcgta ccctctgtcg gtgtgataaa tacgtcgtat ttcagtttga
    15241 cctttagctg ccagagcagc cagtaccagc gatgctcctg cacgaagatc agacatcata
    15301 accggcgcag cctgtaattg gtttccacct ttaacaacag caacatttcc ttcaatttca
    15361 atatcagcac ccatacggcg cagttcccca acatgagtat accggttttc aaagattgtt
    15421 tcgataataa cgctgatacc gggtatggtg cacatgagtg acattatagg cgcctgaagg
    15481 tcggttggga agccgggata cggcaatgtc ctgatcatgc tgccctgcaa ttttttagat
    15541 ggttctattg taatggaatt atcgccgccc gcaataacaa aacccatttc agacaatata
    15601 tcaatacagg cctggaaatg ctcagggata actttgttta tggtaatgtt actacgtgta
    15661 atagctccag caatcagaaa tgttccagct tcaatgcggt caggaataat tgaataggtt
    15721 accggtttaa gcttttttac gccttttata acgatgcgtt cagtgcccaa tccgtcaatt
    15781 tttgctccca ttttgattaa aaaatttcct aaatcaacaa catccggttc catggcacag
    15841 ttttccaata ttgtttcgcc atcagcaagt acagctgcca tcatgatatt agcagtagcg
    15901 ccaacagaaa cctttcgcat tataaaacgg ttcccaatca gtttctttgc gcgtgctttg
    15961 atatatccat gttcaatatt aatggtgctg cctaaagctt caaaacccga aaggtgaata
    16021 tcaacaggac gctccccaat agcacatcca ccaggcagcg atacatcagc ttctccgcat
    16081 cgagccaaaa gtggtcccat aacataaatg gaggcgcgca tggttttaac aaggtcatag
    16141 ggcacttcgg catttttcag actgttgaca tgaatggtta gcgtattcgt ttgggtatca
    16201 aactggtaat cagctcccag gcatttgata acctgcaaaa ttgtttgaat atcttttaaa
    16261 ttgggaacat tatgtaaaac ggttttgcct tcagcaagaa ggcttgctac aataataggt
    16321 aatgcggcgt ttttagcacc ggagatagtt actgaaccat tgagcttttt gccgccattg
    16381 atgatatacg tatccacagt ttcctcacat tttgtattga ttttatttgt gagcagtagt
    16441 aataatgtag caaattgtca attcaattaa aagattttat cttcaaaatt aatgtacagt
    16501 cgctatcaca aatatagtcg tttgaggaat tttataaccg tatgacaaaa aaatatgaac
    16561 tatcgctcca tatattcaga agggatctga gactagacga taacacagcg ctcatcagag
    16621 catgtgactt gtcagattat gtgattccct gttttatttt agatgataga caactttata
    16681 ataatccata tcggggagat aacgcagttc agtttatgat tcaatccctt agggatttga
    16741 ataatgaact ggtcaagaag ggcagtcgat tatatctatt taaaggtatt gcagaagatg
    16801 ttgttgaaaa gttgataaaa gaagagaata ttaacgctgt attttttaac cgtgattata
    16861 caccattcag cacaatgcgt gatggaaaga taagagaaat atgtaaaagg tataacagag
    16921 attgcaatat ctacgctgat gcgcttatga atgaaccgga aatggtggtt acacaggatg
    16981 gcaggccata tactatttac tcacactacc tgaaaaaagc caggatgttt gcagtcagtt
    17041 cccccctgaa aaataaattt aacaattatt ataccaaaaa cataaaatca gaagagccac
    17101 acagtattct tgataccatt atcgtgaatg agaatccaca tctcttttta aaaggtggca
    17161 ggaaagaagc actcaattta ttattaaaaa tagcacattt gcaacattac gatgaaacac
    17221 ggaatattcc cgcagtgcag gggacaagcc tgttatcagc acacattaag tttggtacag
    17281 tatccatacg cgaagtctat cacactgcaa tcacgcattt aaaaagcccg gattcattca
    17341 ttaatgaatt gtactggcgc gattttttta catctattgc atttcatttt ccacatgtct
    17401 ttaagggagc ttttcataaa caatatgatg cgctgcagtg ggcaaatgat aaggaaaaat
    17461 ttgaactgtg gactaatggc ctcacaggat ttccaattgt tgatgcgggt atgcgacagc
    17521 tcaacacaac aggatggatg cataacaggg tccggatgat tgtggcaagt tttctggtca
    17581 aggacctgca tattgactgg cggtggggtg aacgttattt tgccaccaag cttattgatt
    17641 atgatccgtc ggttaataat ggcaactggc aatgggctgc atctacaggg tgtgatgctc
    17701 agccatactt tagaattttt aatccctggc ttcagcaaaa aaaatatgat ccggaatgtg
    17761 aatatataaa acgctggata ccagaattac aaatcgtctc tttaaaagat attcataatt
    17821 gggtatattc tggtagtagt gtgaaatatc caaaacccat attagatcat gttatcgagt
    17881 catcttacgc aaagggaatc tataagtcca tattcagaaa tgaataaaaa aataaaaaaa
    17941 ttcttgcaat ttgctattgt atttctttaa atgtcaataa tgagtgattg ctcactcatt
    18001 attgagaact gttacttgaa ttaaatactg ataacctata tacttaaatt gaaaatatta
    18061 tataatcatt atggaggatt ttatggtaat tggtatacta aaggaaatta tggaaggtga
    18121 aaaacgggtc gcagccactc ctcaaacagt aaaaaaaatg atagctgatg gttataaagt
    18181 actagttgaa acaggagctg gagaaggctc attctataac gatgatgaat ataaagctgt
    18241 aggtgctact ataactgata cagaatcaat ttatcacgaa tctgaagtaa tactaaaagt
    18301 aaaagagcct ttatttaata atatattaaa tattcatgaa gttgatttgt tacactccgg
    18361 tcaaataatt atttcatttc ttcacccggc ctcacctgat aatcatgaaa tgatcaagaa
    18421 attaactgcc aaaggagtaa ctggtcttac tttggatgga gttcccagaa ttagtagggc
    18481 acaatccatg gatgctttgt catcaatgag ttcttgcgcg ggttataaag gcatgctttt
    18541 agcagctaat gcaattgcta aattcatgcc tatgattact tcagctgtag gtgttgtaaa
    18601 accagcaaat gttttggtgg ttggtgcagg tgttgcaggt ttacgaggta tagctacagc
    18661 aaaagggctt ggtgctgtag tgtatgctgc agatattaga cccgatgcaa atgaacaggc
    18721 aaagtccctt ggagctaaaa ttattgatac tggagtacct gctgatatag ctgtcagtca
    18781 agatggtaaa tatgcaaata aactacctga ggaatggtta aaagtagaaa gagagtcatt
    18841 gaaagatata attgctcaag ctgatatagt atttcttaca gcattagtat ttggtaaaaa
    18901 agcacctatc ttagttgacg aagaaatggt taaattaatg caaccaggtt catgtatagc
    18961 tgatatttct atcgatcagg ggggtaattg ttcaataacg caaccaggtg tgatatcaca
    19021 aaaacatgga gttaatataa ttggttacaa gaatattcct ggcatgctgc cagctagttc
    19081 gactgatatg ttttctaata atatatataa tttattaaaa tatctaacag tagatggcaa
    19141 aataaaactg gatttatcag atgaaattgt caaatctatt ttagtatgcc atgagggagt
    19201 attgcttcat gaaggaacat tagaggcaat gagtcaaaaa tagagtttat tgttattaag
    19261 gtgaaatgta tatgacagca ttacaaatga tactattatt aatttttgtt tttacaacgt
    19321 ttataggata tcgtcttatc aaaaacgttc cttcgttatt gcatacccct ttgatgtcag
    19381 gaatgaatgc tttatcgggt gttaccattt taggtgcttt aatcgctaca tcactatctc
    19441 ttgctaatgg aaatgcaatc gtaagctggc ttatgggtgg tattgctatc attttagcaa
    19501 tgataaatgt atgtggtgga tttgcagtta ctgataggat gctgaaaatg ttttcaaaaa
    19561 aggacaatag aaatgaatga tacactacta tcttgtcttt cagttacatc aataaacaat
    19621 gcctattata tttttgcgtt ttttataact attatggtgc tttttggaat ttttctgatg
    19681 agcaaaatta gatatgcgcg ttatggcaat ctactaagtg ctattgctat cgcattggca
    19741 attattgtta cattaatcaa aaaccaaatt ataactacag gaagtctata tatctttgtt
    19801 gtaattggtg cagtaattgg cttgcgcgta gcatataaag ttaaaatgat acaaatgcca
    19861 cagctagttg cattattaaa tggttcggga gctttagcgt ctgcaattgt tggtgcttat
    19921 tcatattttg gtattggtgc aggattggat atgtttacaa gggtaactgc aataattgca
    19981 tttcaggttg gcattattac attttttggt agccttattg ctgcaggtaa attacaccag
    20041 gtaattcctc aggctccagt aatattacct tatcatactc atttttcaat aataattttt
    20101 gcgagtttaa tatgtatcgt aatttgttca gtcaataata ttatagctga ttctttattt
    20161 ctgattgttt catttatttt ttctttgatt tttggtgttt ttttcacaat acgaataggt
    20221 ggagcggaca tgcctattac aatatcatta cttaatagta tgtcaggtat tgcaggtgcg
    20281 atatgtggat tagccatagg agatttttta ttagtttcaa ttggtggcat agttggtgct
    20341 tctggcttat tgctgacaca gattatgtgt tgggctatga ataggaatct tctagatata
    20401 ttatttggga aaactactat tgcagcaaag aaaagaagtg ataacgatat aataaaaggc
    20461 gatcaatcta aaggtgtata taagtcgcag gcaaatccat tagaagttat aaaaaatgct
    20521 aaagatgtca tcatcgttcc tggttatgga atggctatat cacaggctca acatctggtc
    20581 aagcaattat cagatatatt gattgctaat ggagctaagg tgcgatttgc aattcatcca
    20641 gttgcaggga gaatgccagg gcacatgaat gttttattag ccgaagccaa tgttgattat
    20701 gatgatttat atgaaataga taacataaat ccagaattta aaaatgcaga tttggcaata
    20761 attgtgggag cgaatgatgt cgttaatcca gcagccaggg aagcaaaaga tactccaata
    20821 tatggcatgc ctattttaaa tgttgatgaa tgtaaaaata tttttgtctt taactacgac
    20881 cttaagccag gatatgctgg agtagataat ccactatata ttagaaatga gggtatttat
    20941 ttctttctgg gtgatgctgc agaacaatta aagaagttta ttgatgattt tctactttca
    21001 aaagttaatt aaataggaaa gtaaattatt attaaatttt tgataataaa ctttgagcta
    21061 aatgttttgc ctgctctttt gtaagttgaa ttgttgctgt acctccaccc ggatgaggca
    21121 gtgttagtaa aatcttacct tcgtttacct cgattttgta ctgcccatcc ggcattttta
    21181 cttcaggcga caaccagtca tcggcattga attctgcaat ctgtatcaac acaccaaatg
    21241 catgttttgg atggataaag atttctttcc atacaccgcc agggtattca tgataaccaa
    21301 agtaaggaac tcccatttgc tctaattttg aaatggcttt tttaatatca tgagtttgta
    21361 gtgttatgtg atgaacgccg cttcctcgtg attctatgaa accatctaaa aagctccctt
    21421 tgccggttgg tgttattaat tcaattcggc tcatatcacc aagagaaaat atctgccagt
    21481 aaaattttgt actatcatct tttgctccaa ctcccgcaac agcacctaag ttttcaaaaa
    21541 atatttttgc tttatcaaaa tcggggacag caagtgaaac atggtcaata cgtgctatca
    21601 tacattacct cattgttttg tttattgttt tattaaacaa atgttagttt caatatgtaa
    21661 acattgcaac taacaatcag gaaacaggaa ttgaaaaaaa tagtaaatga aaattttcaa
    21721 tgtattgaaa aatttattgc aaattttgca ttattagaca ttgtttagac aataacataa
    21781 atcaaataaa atacaaaatc aatactttgg agggataatg cacatagaaa ctaccacctg
    21841 catttcattg acaaatcttt ccatcttaga gaattatgca aaacagctga atgttccgtt
    21901 acgctcatta atcatgtatc tattgatgta tgctgctaaa aatgaaaaaa gaaaagctat
    21961 agcattcaaa cgcctatcct acaggaaacg taataccgaa gattcctgga ggagagtgca
    22021 tctggtgttg tatcattctg aatatgagtt tattctggat gtaaagaagt tgtggaagat
    22081 gtcactggct cgttgtattg aattttgtat cgataatatt ttgtttgagt ttataaatta
    22141 ttatatggat caattaagaa gaaaagatac cgataactat ctgccttatt atcaaaatag
    22201 atcatacaca ttggactttt atcaggaaaa agggatacat tgcatcacgt gttactgggg
    22261 tccaccaccg gagttactaa ataatacatt atcacttgaa ttagcatagt ttttgcaatg
    22321 taacaataac aacaaaatcc acctttattt tgatcatgta atatgttatt gacattttct
    22381 aatgacgtat aattaatcat tacctctatt gattatatta aaacaagaaa ttttaactgt
    22441 aacgtgctca ttttactttt tatgattacg aggtgtgtat gcagcaaata gtttcttttc
    22501 tttcaaatcc aaaatcctac aatcatcctg ttgatactgt tactgttgta caaactcata
    22561 tttcgtatgt atttattgta aagccatatg tatataagct taaaaaacct gtggattttg
    22621 gttttttaaa ttttatctcg tttgaagaca gaaaattctt cacgtttgaa gaatatcgat
    22681 taaattcccg tattagtcct gaaatatacc tgggtgttgt tgcaattgta aaagatggca
    22741 attcgttcag gttggtagaa gatgctgcat ataatgaaaa cgatgtcgtt gcttattgtg
    22801 tcaagatgaa atatatagat gatgagtttt cgctcaaaaa tttgctgaag cagggtatta
    22861 gtaatcaatt actatcgctc atagctcaaa aaatttatgc gtttcataaa aatgctgata
    22921 atacagaagg attgcaggga tttggtggta tcaacgagat aagtaaaaat acactggaaa
    22981 attttgatca aattgaaaaa tatattggca ttgctatttc taaaaacgat tatacattta
    23041 tgcggtcatg gactgaggat tttattaata cgcatcaaga gttttttaag gatcgcaata
    23101 tacaggggtt tgtacgtgat tgccatggtg accttcactg tgagcatata tactataaag
    23161 atgattcaat catcattctt gattgtatag aatttaataa acggttcagg tatattgaca
    23221 caatttcaga tattgcattt ttgctcatgg acttagatgt aaatggaaac attggtgatt
    23281 caaatagatt gtgtgcacaa tatattcagc tttcagggga ttatgatggt gtgttggtgt
    23341 tgccttttta caaggcatac cgtgccatgg ttagggcaaa aattaatagc tttcagtcag
    23401 ataatgaagg tataacgcaa catgaacgag atgcgtatat acaaaaggct tcacaatatt
    23461 ttcaactggc aaagcattat atgcagcaaa atgcaaaggg tgttgtttat attgtttttg
    23521 gaaacatagg aagcggtaaa agcactatat ccatggaaat ggcttcgcta acaggtggca
    23581 ttgtcattaa ttcggatgcg gtacgcaaaa agcttgcggg gcttgatgtt tttgataaag
    23641 caaattcaaa agctggtaaa ggcatttata ctcctgagat gactgaaaaa gtgtatgcaa
    23701 caatgaaaca aaaagccatg gcgatagctc ttgcaggtat gcctgttatt cttgatggga
    23761 cattttacag caaggaacta cgaagaaatt tttatcaatt ttttaaagaa aaagaaattg
    23821 atgtgcaatt tgtttataca cagtgtcccg aagaagaact tatcagaaga attgaaagaa
    23881 gggaaaaaga tacatcaatt tcagatgccg atgttgagat tttcatgcag ttgaaagatc
    23941 gttttgagaa acccgaagat gatgtgcctg aactatacgt tgtaaattca aatacaagta
    24001 tagaagatat ccggaatcaa ttg
//
LOCUS       DAORYL010000044        23507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1825_length_23507_cov_11.997996, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000044 DAORYL010000000
VERSION     DAORYL010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 23507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23507
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1825_length_23507_cov_11.997996"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..1750)
                     /locus_tag="PLV81_09130"
     CDS             complement(149..1750)
                     /locus_tag="PLV81_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOM10297.1"
                     /translation="MSKKVLLIYPEIPLTYWSFKYSLEFIGKKAVFPPLGLLTIAAML
                     PEDWKVDLIDLNISTLDPEILPHYDMVFISAMLVQKKSFDMITSLCKDYNVPVVAGGP
                     YPTSLWHSIPNVNHFVLNEAEVTLPKFLHDLHYGIPHHCYTSNEKPSLDKTPVPRFDI
                     IDLTLYSSMPLQFSRGCPFNCEFCDIISLFGRVPRTKSPQQFLAELDAVYATGFRESI
                     FIVDDNFIGNKTRAKELLKAIIEWQIDHSYPFSFQTEASINLAHDEELMQLMYKAGFD
                     MVFLGIETPVIASLESAGKTQNTKEDLLENVKIIQQHGMEVSGGFIVGFDDDPENVFD
                     LQVEFIQSSGIPIAMVGILDALPQTRLYDRLLSEGRLLYESNGNNTHKLAPNFIPKMP
                     VQKLIDGYIQLLKTIYSPRNYFERCLTFISRIPQERMVYRQFTFKEFKALVLSLIKQG
                     FSYYFMYYFKFLLNTLLLRPKLFSEAIALAIKGYHLFKITEILLDEYNKSLKEEEHTD
                     TFAPSFASTFTTSTDTAGMVHTDNP"
     gene            complement(1881..2954)
                     /gene="ilvC"
                     /locus_tag="PLV81_09135"
     CDS             complement(1881..2954)
                     /gene="ilvC"
                     /locus_tag="PLV81_09135"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOM10298.1"
                     /translation="MQYNLLHTRVLHLLYKHITIRGNVMAKMYYDNDADLSVISGKTI
                     AVIGYGSQGHAQAQNLRDSGCKVIVAELPGTPNYDLAVSHNFKPVSAKEAAEKGDIIQ
                     ILAQDHVQAKLYETEVKPHLKKGKTLVFSHGFNIHFGQIVPPADVDVIMVAPKGPGHL
                     VRSEYEKGGGVPCLIAIHNDASGKAKETALAYAKGIGATRAGVIETTFKEETETDLFG
                     EQCVLCGGVSELVKAGFDTLVEAGYQPEIAYFECLHELKLIVDLFYQGGINYMRYSVS
                     DTAEYGDYMVGKRIVTPETRKEMKKVLEEIQSGQFARQWILENLAGRPQFNAIKKRER
                     EHLIERVGRELRKMMKWIDSKEA"
     gene            3081..4133
                     /locus_tag="PLV81_09140"
     CDS             3081..4133
                     /locus_tag="PLV81_09140"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HOM10299.1"
                     /translation="MSKKTIHDKAYKYLFSNPLIVKELLESFVAMDWVHYIDFNSAQP
                     IDKSYVNDDYREYEADIIYKLQCKDSEIYLYLLIEFQSTVDRFMAFRMLNYVMELYRE
                     LYYKQGQKRLPIVFPLLIYNGDKKWNAATELQQLIDIPEEFKQIKKYLPQFKYYPIIE
                     NELSRNELENMMNIVATIFLFEGSDKEKFLKTIDRIEKLMQIYSKDSTVIRTLLVWLL
                     HYLKTHSIIGDIEPFIENKLHPEETYTMLSKTLETLKEDLRKEGYQEGLKLGIQEGLQ
                     KGLQQGLQKGLQKGLQKGLMQGIVKGKKEGLMLGITKERLKIANKMLRNGIDDNTILK
                     ITKIDKKELEQLKKKI"
     gene            complement(4155..4631)
                     /gene="ilvN"
                     /locus_tag="PLV81_09145"
     CDS             complement(4155..4631)
                     /gene="ilvN"
                     /locus_tag="PLV81_09145"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOM10300.1"
                     /translation="MSQHVISVKVENKFGVLARVAGLFSARGYNIDSLAVSETESPDV
                     SVMTIVVRGDDRIIEQVKKQLNKLIDVIKVTDHNPGNAVQREIVLAKLNVTPSKRNEV
                     YQLAQIFGARIAGVSNKTIILEMVNDPETIDNFIELLRPYSIKELIRTGRVSILKE"
     gene            complement(4628..6310)
                     /gene="ilvB"
                     /locus_tag="PLV81_09150"
     CDS             complement(4628..6310)
                     /gene="ilvB"
                     /locus_tag="PLV81_09150"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOM10301.1"
                     /translation="MVKLTGAQIIVESLVKEKVEIMFGYPGGVVIPIFHHLFNAPIKF
                     ILTRHEQGAIHAADGYARATGKPGVVIATSGPGATNLVTGIATANMDSVPLVIFTGQV
                     STAVIGNDAFQEADITGITRPITKHNYLVNDVKDLARIIKEAFYIATTGRPGPVLIDV
                     PVNISNSETEYHYPETVHLRSYKPVIHGHAGQIQKACKLIEQSKRPVVYAGGGVISSN
                     ASEELRAFVKKTGIPITTTLLGQGAYPETDRLSLKMLGMHGTYYANHAMHQSDLVIAI
                     GARFDDRVTGKIDEFIPHAKVIHIDIDPSSVSKSVAVDIPIVGDAKHVLAEMNKIVKA
                     PKIDEWVAYVMKMKDENPLTYKDSDVKIKPQYVVEKIYELTKGDAIICTEVGQNQMWA
                     SQFYNFSKPRTFISSGGLGTMGFGFPAAIGAQLGQPDKRVIDIAGDGSIQMNIQELIV
                     AVQHRLPIIIAILNNGFLGMVRQWQQLFYNKRYSYTCINFQPDFVKLAEAYGAIGIRI
                     EKKADVVPAIEKALSITDKPIILDFHVDREENVYPMIPAGKSVKDMMMRELA"
     gene            6753..7709
                     /locus_tag="PLV81_09155"
     CDS             6753..7709
                     /locus_tag="PLV81_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOM10302.1"
                     /translation="MSQNERLQYIDTAIREKGVVTVQEVVSKFEVTPRTVKRDIEYLR
                     ERVNAPIQYISHKKGYCYSLPFDYFTYRNERMLLFYIFLTRLIDSPTHFPFIDRTVIS
                     NIKKSIKATYLPLITRITYEIEEWEELDIELFSKLFAAMRDGKVVTIQYADVKGKPST
                     RAIAPEHFIHYEGRWYCIAYDEHKKGIRTFLLSRIRKAQVDVKRRSKKLSNEEIQKYI
                     QSTYGIYKGKTGQVVCIRFYEPVSNIVKKQVWHREQKMKEGSHPQFGAYVEMLLPVHH
                     PQELLGKILRYGKYAEVISPEDIRQQWVNAIAETYKRYCEKK"
     gene            7792..8007
                     /locus_tag="PLV81_09160"
     CDS             7792..8007
                     /locus_tag="PLV81_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10303.1"
                     /translation="MKDYLIELISSMFWIVWFVWMSDTEIILNVYVWLGIILFVGWYF
                     STDKKIPVTVQIAVWLCFFILDILFRW"
     gene            complement(8052..9077)
                     /locus_tag="PLV81_09165"
     CDS             complement(8052..9077)
                     /locus_tag="PLV81_09165"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOM10304.1"
                     /translation="MEPAKRRRDIPVLLLHDIDPQWELHEQEAAHQSAQILINELQNE
                     GHSVVDVPVTHHSLDKVLKSYNPNDYILFNWCEGLPGVDHSEHTVAQILEEKQFVYTG
                     SPSDILFLCWNKAAIKKLLQIKNIPTPDGIVTDSTSVIDWYKFPAIVKPAFEHCSIGI
                     TEDSVVTNRKELLDRIDYILSNFKQPAIVEDFIDGREFHVTLWGNGVIHTLPPAEMDF
                     SAFDNIKDRVCTYNSKFKPGTSHYENITLKIPAQLDNNQLAILNETALQAYRALGCRD
                     YARIDIRLKDGIFYILDINPNPDFSPDTSTVYTAEHAGLSYGAFASCLINLAALRHHV
                     YGEKITI"
     gene            9283..9585
                     /locus_tag="PLV81_09170"
     CDS             9283..9585
                     /locus_tag="PLV81_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10305.1"
                     /translation="MKRKYFLFNKNDILMVIVIVTNVLSLCYIVQNFLVMIFGVFITG
                     ILFLGWVAYNRLTRYHKFIGYTRKLAEKTLWTTTVTINLNDIPKCPSTSMEIHPND"
     gene            9622..10404
                     /locus_tag="PLV81_09175"
     CDS             9622..10404
                     /locus_tag="PLV81_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10306.1"
                     /translation="MLHTYYDNPDGEVFIYNNNAYFFKYPFIENEPELIEENVVYYTE
                     YKIPDSRSYRSKQYDDILVVYKDGSINGQITGLNFYLKDAFKLSVYDSSILCIAPHTI
                     LYVDTERGIQFERNYKGFITDGWLEYCDYNDYIQYLLIVNGKIVYGNNTMTMTMNRKI
                     VTYDQLVSTFEDGFVYNRTFYDIGATPKKVFCFGSYCLAFIHDGLYFFEFVNNRAKTA
                     FLIENIEDIFYSYKEKFFYYYDTHGEINLWNPGDLKELGFLS"
     gene            10615..11253
                     /locus_tag="PLV81_09180"
     CDS             10615..11253
                     /locus_tag="PLV81_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOM10307.1"
                     /translation="MPRTARNVEEVDIFKQQILDAAVKIVIREGFARLSMRKIAAALG
                     VTATTLYNYYTNKDELYFYIRIRGFELLYGLFEKAYTSASDSRDKIKSLIASYIEFGI
                     QYPDYYEVMFLNRNVPKFLDCIGTPLEVVGAKEKEIALKPFAFVIEKLQQHCELDPDS
                     AYVTALQLWIECNGVVSLYNSRLIREVHDNPQRFVDSYVDVIAQRYIPLDIK"
     gene            11266..14253
                     /locus_tag="PLV81_09185"
     CDS             11266..14253
                     /locus_tag="PLV81_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOM10308.1"
                     /translation="MLLFTSQSKFNTIGTGIFIKSFLKFIAFNFNNRKSLNRYLKTKD
                     GYLNFTIGLRTEDNSITCSIAFMDGRVTVSGTIAPDANAVLIFKNEDAARKLLLGTPT
                     DIIYMLLKSDVRIDGSITYSNIFSFFISLLFNNVQKRAMKKERQADEKKVKKEHPKPH
                     KELSDEIARRRKERLKGKKEDPDVLYLEDPYLSHYCLDDFPRIKKFLDDHLTKKPAIC
                     AERAILLTQWYREHGFDTDNNGTPWDPVLRNAYAYKYLMEHKKPVIQKDSLLPGTTTS
                     KPIGSPIFPDSTGIMIWNELLTIPYRTYNPFDIDDQTREILHFEILPFWLHRNFKEWV
                     REKYNNPLCEQLDERFALYFDWKQATISHTIPNFPKVMAMGTSGMIDEIKAELANDCS
                     QEKRNLLTAMIACLEGINAYAKNLSQQALSDSNQETDPIRKQELLKIADICSRIPVNP
                     CITLHEALTAMWITWIALHNESLNAGLSLGRLDQWLQPYFINDMKNTPPEKREEYIKQ
                     AIELVADFYMRCTDHFPLTPDLANFYFGGSSSDQAITLGGVTPDGRDAVNDMTYIFLK
                     VTEMLSIRDPNVNARYMPGVNSDTYLKRLCEVNLITAATPSMHNDAAIIKALTPLGYD
                     IQDIRDWSATGCVEPTLSHKHFGHTNMQMMNMVAALEMALNNGYHPLTRLDIGPKTGR
                     PEDGVFKTFDDFFEAFTRQFAFIIDQSIDYNNTLAEAHQYLRPTPLLSAFIEGCLEKG
                     RDVTKGGAKYNSSGTAIIGLADVTDSMMVVKKLVYDEKKYTFADIKKAIDTNFQNDPV
                     LLATVHKKVPLFGSGNEEAVAMANRIAKWAHDYFASIPHYRGGTYTTGFWSMSNHVAF
                     GTLTGALPSGRLAGKPFTPGLTPQPFASKNLLDNIRDVAKLDPVNLTNNIAFNVKVVP
                     SAIESREKTVHDMFSYVKTYFTLGGMQMQMNVVTSAVLRDAMAHPENYRNLIVRISGY
                     NAYFVTLNRHMQLELIERAEYGI"
     gene            14273..15178
                     /locus_tag="PLV81_09190"
     CDS             14273..15178
                     /locus_tag="PLV81_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOM10309.1"
                     /translation="MKTLILEIKGNSLDDGPGIRTVVFFKGCPLNCVWCHNPESKKAV
                     IELSWDKKECIACLSCAKVCPEHAIDLKYQNFVDRKKCTLCFKCVDVCPSGAMSTVGK
                     PMTVDDIMQHVLKDKPFFDTSGGGVTLSGGEPLLNIPFVSALLKQCRNNAIHTLIETS
                     GYFNWNEFESCIAPYCNTVYYDIKLIDEEKHKMYCGVSNTKILSNFKKLIQLSQSMGI
                     QIVPRIPLVPGITATEQNLTGIAKFLHDCGVDNVELLRYNPLWPEKCMKIGNNAKVDE
                     ALTRWMPHEEYERCKKLVSGVTITL"
     gene            complement(15196..16185)
                     /locus_tag="PLV81_09195"
     CDS             complement(15196..16185)
                     /locus_tag="PLV81_09195"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOM10310.1"
                     /translation="MKQDNLPFICFFGCGYIAKRHAKIIQKLYPGIRLGFSSLYEKDA
                     KEYAAQFNGIAFKNYEEAAQSDCDIAFITTPHAYHKDLAILAANYKKHVIVEKPATRN
                     LKEFDAIVKAVTKNNVRFTVAENYYFKPFLKIIHKHIANGSIGKPLYIRLTKHNHDTI
                     TGWRTDAALMGGGALLEGGCHWVNELVSLAGATPVAVQAIKADVAYQTNIPFEDTIHI
                     TVKFDNGTFGTLFHSWFVSNTFKGMALSKIYGTEGNITFESNGLFTFVNGNRFALRFS
                     FSDFLGFGAMHRSFIDNYRTQQPWEPDYLRIRKELALIEAAYRSLKTHKFEEI"
     gene            complement(16190..17752)
                     /locus_tag="PLV81_09200"
     CDS             complement(16190..17752)
                     /locus_tag="PLV81_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HOM10311.1"
                     /translation="MKTIKSSYEYVIIGSGFGGSFPAYELAKAGKEVCIVERGVWVKR
                     DDTCWDEYALHLQKKPLYQGYTPYLVDQQGKIEADWPYDTVGGMSTFYGAAAFRLRVQ
                     DFEGPPQPDTDKRKSELAWPFGYSTLSPFYDKAEELQCVAGIRGQDITEPPRKKDYPQ
                     KPPLQLSRPSQKIWDAATQLGLHPFYIPMAINFTGKCNNTPCILCNTCDHYLCKIQAK
                     MDLSVSVLPKAIKHGAIVLDNHRAVKINFIKNKAQSVDIINQATGQRHTIKCKYLIIA
                     AGPIATPHLLLASGVESVSSATQHIGHNLMRHINGVVSGVVPYIANPDNTLAKRVAIA
                     DYYFGDSSGKSSPKGNWGIIQDIATPGKGVIKANAPFGLKNIAATFSQFLINQLVIAE
                     DIPQYDNRVYCADTRDSFGMPNVAIYHRYHARDIAVRDALYNVAKRILKQAGAKIFYY
                     MPIVTFSHALGTCRMGNNRNLSAVNPECRLWDITNCYITDASVLPTGGSVNPSLTIAA
                     NALRVANIMLKR"
     gene            complement(17781..18281)
                     /locus_tag="PLV81_09205"
     CDS             complement(17781..18281)
                     /locus_tag="PLV81_09205"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOM10312.1"
                     /translation="MQLGHVAVSFIISSYAPQITGGAMQAFSLESILVAFGAHWLPNL
                     DVIPIKLGWAKDSFHCTWSHSLIFAFAVALILLPFNSAWAALAFVSLIIHYLADMPSS
                     VGLPLLMPFTNKRFTINLWADTGHSGWDALIGTYVQAWTWILEGGAFVFLFVRMYQLG
                     VWPFIS"
     gene            complement(18330..19022)
                     /locus_tag="PLV81_09210"
     CDS             complement(18330..19022)
                     /locus_tag="PLV81_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOM10313.1"
                     /translation="MDGTLLRTHGAGKQAMVQAAIEVYGTAGDMELVNFQGRTDPLIL
                     MQSLIPHGITEETIFAYIEDFKQRYFTYLKNIMPHSEAILLPGIHELLKALTEFGNVI
                     VGLLTGNFYQGALIKLQHFDIFKYFSMGVFADDTHIRNEMPFIALQRLYSLTGRTFKP
                     EDLIIIGDTIYDIECGKTSGAITVAVATGWTDAQTLLQHKPDYFFQDLSDTRAFLYTI
                     KELHDCIELKVQ"
     gene            complement(19040..19270)
                     /locus_tag="PLV81_09215"
     CDS             complement(19040..19270)
                     /locus_tag="PLV81_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10314.1"
                     /translation="MLPRTPVLAMFFAMIIAILIVLIFEIPGRPFTIRLAIWLYTLSL
                     LPGLGIAMLFDPAPRAAKERFLKINFYEEKKS"
     gene            19611..19979
                     /locus_tag="PLV81_09220"
     CDS             19611..19979
                     /locus_tag="PLV81_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10315.1"
                     /translation="MPAHTIKSKTLLTVFKGIIAQDDVNENDVYQQIQSYLTLTPFYV
                     KLFMFFSLVYCNLLSVVTNQKMIYSLPGNKVAVLLNNCEKHFIGRSIIMLLKLICTLV
                     YFDDDKHAERIGYRHLVHCK"
     gene            19989..21494
                     /locus_tag="PLV81_09225"
     CDS             19989..21494
                     /locus_tag="PLV81_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HOM10316.1"
                     /translation="MIYTLKDYKKNYKNIFDADVCIIGSGAGGAVAAYELAAIGYSVV
                     VVEEGGYFTTKDYDGKPLNNMKTMWRQAGSTVSFGIPPISIPTGKCVGGTTAINSATC
                     FRTPEHIIKQWKENFKVDIDYKSLEPYFERVENIINVTELPWDVLGNCAKIVKRGADA
                     LGLHCKPLKHNVKNCKGCGTCQFGCAEGAKQSAEISYIPLALQYNTSLLTHCRAEKIH
                     FKGMKADSVECRVYNEKGESVASCTIKAKIIVCACGTMITPLLLKKSGIKNKHLAKHL
                     QIHPCSRVVALMEEEVNGWVGVSQGAYIDDYADEGIMLEGIFVHPSILAASLPGVGKE
                     FFELTKQFSHIAAFGVMVHDTSEGAVFGNMHTSFSWYMMNTKDIQTLKNGIAYLAQIF
                     MAAGAKKIFTPISTFPVISCQDDIEKLKQTYLMRSQIPEVFAFHPLGTCRMGGDSDCG
                     VVNSYGKCFNTDNLYVADGSIVPTSLGVNPQITIMALSTRIAGHIAEQLNN"
     gene            21507..22181
                     /locus_tag="PLV81_09230"
     CDS             21507..22181
                     /locus_tag="PLV81_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10317.1"
                     /translation="MNRIKIICVLVILVFGLLVLYHNAYADVDNSLLKQADIQSLREG
                     HIVIKKQKSKTQEGEIARVAGAILIQKPIQEVWKCILDWERMPQYVDTLDYYTVLAKI
                     NHKTSIIGGQIHVAFLKFNYTLLVSNDERNYYQKWQLISENDIVQYNIQKNIKPASTG
                     IKNIEGYQYCIPLDDNNTIVYYAPVVEVSVPVPGFVENNLTQKSIKDYLYGIKRYLEG
                     GHNEKK"
     gene            22168..22614
                     /locus_tag="PLV81_09235"
     CDS             22168..22614
                     /locus_tag="PLV81_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10318.1"
                     /translation="MKKNRIIQIITITGLLYAIAFIITTIMFIFFNSTLINTINVLSQ
                     KLIPALPLAQEHSQFFLILSVSMMSGVTVCSLLLYKNAELYIEMAIPLITMKFTSSVF
                     GLLFFVYGCIYHNGWNTMANLIIFTTDFPLGLWVLYVYRLFKQQKL"
     gene            22624..>23507
                     /locus_tag="PLV81_09240"
     CDS             22624..>23507
                     /locus_tag="PLV81_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10319.1"
                     /translation="MNKEELIASANYTRYIPGKSGHYESFFVRANHPHKPLAFWIRYT
                     IFQPKGKPENAIGELWAIIFNEENDKHIALKAEYPISHCSFSKDDLNIVIGDAVLTPG
                     ALQGTITYNNSQKIQWDLTFTGNEESVFLFPVKLYTTKLPKAKSLVGLPNALFNGMIN
                     VNGAIITVSNWVGSQNHNWGLKHTDHYAWGQVCGFDNEPDAFLEIATARIKLGPLWTP
                     FMTPIVLRYKGKEYTLNSLGETFCRAHFGYFYWDFNARSKDISLTGSIFALNKDFVCL
                     PYYNPPGGIKYCLNTKIAS"
BASE COUNT         7552 a         4100 c         4591 g         7264 t
ORIGIN      
        1 cctttttcaa tgagaagcat cttttgttta tgattgaaat acaggaaaaa gaagataaca
       61 acacttcctg ctacaatacc aactatggga atgacagtca ctaatatctg aacagcttct
      121 gaaggtgttg acatgatgca aaggcctcct atggattatc agtatgtacc atcccagcag
      181 tgtcagtact ggttgtgaat gtgctggcaa atgatggtgc aaatgtatca gtatgttctt
      241 cttctttcaa ggatttatta tattcatcca gcaatatttc tgtaatttta aaaagatgat
      301 atcctttaat ggccagtgct atagcttctg aaaatagctt tgggcgcagg agtagtgtat
      361 taagcaaaaa tttaaaataa tacataaaat aatatgaaaa accctgtttt atgagcgata
      421 gtaccagggc tttaaattct ttaaatgtaa attgcctgta aaccatacgt tcctggggaa
      481 ttctgctaat gaaagtaagg cagcgttcaa aatagtttcg tggcgaatat attgttttta
      541 aaagttgtat gtagccatca ataagcttct gtaccggcat tttagggata aaattgggtg
      601 ccagtttgtg tgtattgtta ccattagact cgtatagcag tctgccctca ctgagcaacc
      661 ggtcgtaaag gcgagtttgt ggcaacgcat ctaatattcc taccattgca attggaatgc
      721 ctgaagattg aataaattca acctgtaaat caaagacatt ctcagggtca tcatcaaatc
      781 caacaataaa tccaccgctc acttccatgc catgctgctg tataattttg acattttcca
      841 aaagatcttc tttagtattc tgtgttttac ctgcactttc tagcgaagca attaccggcg
      901 tttcaattcc taagaatacc atatcaaaac ctgctttata catcaattgc ataagctctt
      961 catcgtgcgc taagttaata cttgcttctg tttggaatga aaatggataa gaatggtcaa
     1021 tctgccattc aataattgcc tttaagagtt cttttgctct tgttttattt ccaataaaat
     1081 tatcatccac aatgaagatt gattccctga atccagttgc atacacggca tccagttctg
     1141 ctaaaaattg ctgaggagat tttgttcggg gaactctacc aaacaatgat atgatatcgc
     1201 aaaattcaca gttaaaaggg cagccacggg aaaattgcaa tggcatgctg gaatacagtg
     1261 ttaaatcaat aatatcaaat cgaggtactg gagttttatc caatgatggt ttttcgttag
     1321 aagtataaca atgatgagga ataccataat gcaaatcatg taaaaatttt ggcaatgtta
     1381 cttcagcttc attgagcaca aagtggttta cgttgggtat ggaatgccac aatgaggttg
     1441 gatagggacc tcctgcaacc acagggacat tgtagtcttt gcaaagtgat gttatcatat
     1501 caaacgattt tttctgaaca agcattgctg aaatgaaaac catatcatag tgtggcagga
     1561 tctccggatc aagtgttgaa atattcaaat caattaaatc aactttccag tcttctggaa
     1621 gcattgcagc gattgtcaat aaccccagtg ggggaaaaac agcttttttg cctataaact
     1681 ctaatgagta tttaaaactc cagtaagtaa gcgggatctc aggatatatt aataatactt
     1741 ttttactcat aacaacctct ctatttatat ccggtagtat ttttacggtg gtttttatat
     1801 taagacagga gaggtagggg gagggttgca aaaaaatata ttacagggtg caattacaaa
     1861 gaattgcacc ctgaaaaaaa ttatgcttct ttactatcga tccatttcat catttttctt
     1921 agttcacggc caacacgttc aatcaggtgt tcacgttcac gcttctttat agcgttaaat
     1981 tgcggacgtc ctgcaagatt ttccagtatc cactggcggg caaactgacc tgattgaatc
     2041 tcttccagaa cctttttcat ttctttacgg gtttcaggtg ttactatacg tttaccaacc
     2101 atataatcac catattcagc agtatcggat actgaataac gcatatagtt gataccaccc
     2161 tgataaaaca ggtccacaat cagtttaagt tcatgtaaac actcaaaata tgcaatctca
     2221 ggctgatatc cagcttccac cagtgtgtca aatccagctt taaccagctc tgaaacaccg
     2281 ccacacaata cgcattgctc accaaagagg tcagtttcag tttcttcttt aaatgttgtt
     2341 tcaataactc ctgctcgtgt tgcaccaata ccctttgcat atgcaagggc tgtttccttt
     2401 gctttaccac tggcatcatt gtgaatagca ataaggcatg gaactccgcc acctttctca
     2461 tattcgctgc gtactaagtg tcctgggcct ttgggtgcaa ccatgattac atctacatct
     2521 gcaggaggga caatctgtcc aaaatggatg ttgaaaccat gggaaaatac cagtgttttg
     2581 ccttttttaa gatggggctt aacttcggtt tcatataatt ttgcctgtac atgatcctgg
     2641 gccagtatct gaatgatatc acctttttct gcagcttctt ttgcactcac gggtttaaaa
     2701 ttgtgtgaaa cagctaaatc ataattgggt gtgcctggaa gttcagcaac aattacttta
     2761 cagccactat cgcgtaaatt ttgcgcctgt gcgtgaccct gactgccata tcctatgacg
     2821 gcaatagttt ttcctgaaat taccgataaa tcggcatcat tgtcataata catctttgcc
     2881 ataacattac ctcgtattgt aatatgttta tagagtaaat gaagtactcg tgtatgcagc
     2941 agattatact gcatagtgta tttgcgtatg tgtattatgc aataaaaatt taaaaatttt
     3001 ttaaaaacat aaatcttttt tttaaattat tttagtctca agcgtattta tatataaaag
     3061 tcaaaatgaa gggaacccat atgtccaaaa aaacaattca tgataaagcc tataaatatt
     3121 tattttctaa tcctttaata gttaaggaac ttttggaatc atttgtagct atggattggg
     3181 tacactatat tgattttaac agtgcacaac ctatagacaa aagttatgtt aatgatgatt
     3241 atagagaata tgaagctgat attatataca agctacaatg taaagatagt gaaatatatc
     3301 tatatctgct aattgagttt caatcaacag tggataggtt tatggcattt agaatgctaa
     3361 actatgttat ggaattgtat cgtgaacttt attataaaca aggtcagaaa cggttaccga
     3421 ttgtttttcc acttttaata tataatgggg ataaaaaatg gaatgcagct acagagttac
     3481 agcaattaat tgatattcct gaggaattta agcagataaa aaaatattta ccacaattta
     3541 aatattatcc cattattgaa aatgaacttt cgcggaatga attggaaaat atgatgaata
     3601 tagtagcaac aatatttctt tttgaaggca gtgacaaaga aaaattttta aaaactattg
     3661 acagaataga aaaattaatg cagatttatt ctaaagatag tacggtcata cgaaccttat
     3721 tagtatggct attgcattat ttaaaaacac atagtataat tggagatatt gaacctttta
     3781 ttgaaaacaa actgcatcca gaggagacat ataccatgct atcaaaaaca ttagaaacat
     3841 taaaagaaga tttgagaaaa gagggttatc aagaagggtt gaagctgggt attcaggaag
     3901 gcttacagaa gggtttgcag cagggattac agaaaggatt acagaaagga ttacagaaag
     3961 gacttatgca aggtattgtg aaggggaaaa aagaagggtt aatgcttggt atcacaaaag
     4021 aacgcttaaa aattgcaaat aaaatgctca gaaatgggat tgatgacaat actatactca
     4081 aaataacaaa aattgataaa aaagagttgg agcaactcaa gaaaaaaata taaacactgt
     4141 aaactcagct atcgctactc ttttaatatt gaaacccttc ctgtgcgaat cagttccttg
     4201 atggagtatg gccgcaataa ttcaataaag ttgtcaatag tttctggatc attaaccatt
     4261 tccagtataa tagttttatt tgatacaccg gctattcgtg caccaaatat ttgagctaac
     4321 tgataaactt cattgcgttt ggatggagta acattaagct tggccaggac tatttcgcgc
     4381 tgcactgcat tgccaggatt gtggtcagtg acttttatta catcaatgag cttgttgagt
     4441 tgttttttta cctgttcaat gatcctgtca tcaccgcgta caactattgt cataaccgat
     4501 acatctggcg attcggtttc acttacagca agcgagtcaa tattgtaacc acgagcagag
     4561 aaaagacccg caacgcgtgc aagaacacca aatttgtttt ctactttaac tgatattaca
     4621 tgttgactca tgccaattcc ctcatcatca tatcttttac cgattttccg gcaggtatca
     4681 ttgggtatac attctcttcc ctgtctacat ggaagtccag gataatgggc ttgtccgtga
     4741 tagataaagc tttttctatt gccggtacaa catctgcttt cttttcaata cgaatgccaa
     4801 tggcaccata tgcttcggcc agcttaacaa aatcaggctg aaagttaata caggtatatg
     4861 aatatcgttt attgtaaaag agttgttgcc actgtcgtac catgcctaag aaaccattat
     4921 tgagtatagc gataattatg ggcaaacggt gctgtacagc gacaataagt tcctgtatat
     4981 tcatctggat tgagccgtcc ccagcaatat ctataacacg tttatcaggt tggcctaatt
     5041 gagcaccaat tgctgctgga aacccaaatc ccatggtgcc cagcccacca gatgaaataa
     5101 atgtccgtgg ttttgaaaaa ttataaaact gtgatgccca catctggttt tgtccaacct
     5161 cagtacagat gatggcatcg cctttggtga gttcataaat tttttcaaca acatactgag
     5221 gttttatctt tacatcacta tctttatagg ttaatgggtt ttcatctttc atcttcataa
     5281 cataggcaac ccattcatct attttaggag ctttaactat tttattcatt tcagccaata
     5341 catgttttgc atcgccaact atcggtatat caactgctac acttttgctg actgatgatg
     5401 gatcaatatc aatatgtata accttggcat gaggtataaa ctcatcaatc ttcccggtaa
     5461 ccctatcgtc aaatcgagca ccaatagcta taaccaggtc tgattggtgc attgcatgat
     5521 tggcgtagta ggtgccatgc attcccaaca tcttgagtga aagcctgtcg gtttcagggt
     5581 atgcgccctg tcccagtaaa gttgtggtaa taggtatacc agttttttta acaaatgctc
     5641 gcaattcttc ggatgcgttg gaagaaataa caccgccgcc agcataaacc acgggtcgtt
     5701 ttgattgctc aatgagcttg catgcttttt gaatttggcc agcatgccca tggataactg
     5761 gtttatagct tctaagatgc acggtttcag ggtaatggta ttcggtttca gaatttgaaa
     5821 tatttactgg tacatcaatt aatacaggac ctggcctgcc ggttgttgca atatagaatg
     5881 cttctttaat aatgcgtgca aggtctttga catcattgac aagataattg tgcttggtaa
     5941 ttggtcgggt aataccggta atatcggctt cctggaatgc atcgttacct attactgcag
     6001 tagaaacctg cccggtgaaa atgacaagcg gaacagaatc catattggct gtagcaatac
     6061 ctgtaacaag gttggtggca ccaggtcccg atgtggcaat aacaacgccg ggcttgccag
     6121 ttgcacgtgc gtatccgtca gctgcatgta ttgcaccctg ctcatgcctg gttaaaataa
     6181 atttaatagg tgcattgaaa agatgatgga aaataggaat gaccacccca ccgggatagc
     6241 caaacatgat ttcaactttt tctttaacaa gcgattcaac aattatctgt gcacctgtaa
     6301 gtttcaccaa taacacctcg cacaaaaaag tatttagatg tatatctaaa tataccagct
     6361 atttttattg caacataatt ttttgttaaa catcaaaaaa tataaatgaa tatataaaat
     6421 ataatataaa catatgctaa tttataagca ctattcatat aaatactttg ggcgatagtt
     6481 actgctaatt gatacactgg tatgtagtgt gatatttctt attatacagc ctattgccgt
     6541 gttcatagca gaataaagat gtatatacga tttataaagt tattgtaagc ctttacatat
     6601 tcgtgacatt gcaaattttc tatataaact aataagaaat tgatagtatg agttatacat
     6661 atatatagat aaattataat tgacgttaca actaaatttg ttataataat cttcattcaa
     6721 attttcaaac aacattgaaa cagaggtttg ccttgagcca gaatgaacgg ttgcagtata
     6781 tagataccgc cattcgagaa aaaggagtag ttacggtaca ggaggtggtc agtaaatttg
     6841 aagtcacacc ccgtacggtg aaacgtgata ttgaatatct cagagaaaga gttaatgccc
     6901 ccatacagta tatatcccat aaaaaagggt attgctattc attgccgttt gattatttta
     6961 cctaccgaaa tgaacgcatg ctgttgtttt atatatttct aactcgtctt attgatagcc
     7021 caacacattt cccgtttata gatagaactg taataagcaa tataaaaaag tctatcaaag
     7081 ctacttacct accgctcatt acacgaataa catatgagat agaagaatgg gaggagctgg
     7141 acatagaact gttcagtaaa ttgtttgcgg caatgcgtga cggtaaggtt gtaaccatac
     7201 agtatgctga tgttaagggt aagccaagta caagagctat cgcccctgaa cattttattc
     7261 attatgaagg caggtggtat tgtatagctt atgatgaaca taaaaaaggg atacggacat
     7321 ttttgctttc gcgcatcagg aaagcacagg tagatgttaa aaggcgcagt aaaaaacttt
     7381 caaacgagga aattcaaaaa tacatacaat ctacctatgg aatttataaa ggtaaaacag
     7441 gccaggtagt gtgcatacgg ttctacgagc ctgttagtaa tattgttaaa aaacaggtct
     7501 ggcacaggga acaaaagatg aaggagggca gtcatcctca atttggcgcc tatgtggaaa
     7561 tgctattgcc cgttcatcac ccacaggagc ttttagggaa aattttgcgc tatggcaaat
     7621 atgctgaggt cataagccct gaagacatac gccagcagtg ggttaatgcc atagcagaga
     7681 cttataaacg gtattgtgaa aaaaaataat atcagggaca ttatatgtcc ctgcttgcct
     7741 tataagtttt ttcataattt taaataaata tattatcgtg gaagggtgta tatgaaagac
     7801 tatcttatag aattgatatc gtcaatgttc tggatagtat ggtttgtttg gatgagcgat
     7861 actgaaatta tccttaatgt atatgtctgg ttaggaataa ttttgtttgt ggggtggtat
     7921 ttttccactg ataagaaaat acctgtaact gtgcaaatag ccgtttggct gtgcttcttt
     7981 atattagata ttcttttcag gtggtaatta aagggattta tgaacttata atgactgtta
     8041 atggtaatgg attatattgt tattttttcc ccgtaaacgt gatggcgcaa tgctgctaaa
     8101 tttatcaggc agctagcaaa tgcaccatac gaaagcccag catgctcagc tgtgtatact
     8161 gttgatgtat cagggctgaa gtcagggttg ggattaatgt caagaatata gaaaataccg
     8221 tcttttaagc gaatatcgat gcgtgcgtaa tcccgacagc caagagctct gtatgcttgt
     8281 aacgctgttt cattcaatat tgcaagctgg ttattgtcca gctgtgcagg gatttttaac
     8341 gtgatatttt cataatgtga tgtaccaggt ttgaattttg aattataggt acataccctg
     8401 tctttgatat tatcaaacgc gctgaaatcc atctctgcag gagggagggt atgaataacg
     8461 ccattgcccc ataatgtcac atgaaattcc ctgccatcaa tgaaatcttc aacaatagca
     8521 ggttgcttga aattggaaag aatatagtca attctatcca gtaattcctt tctgttggtt
     8581 acaactgaat cttcggtgat gcctatgctg caatgttcaa atgcaggttt tacaattgca
     8641 gggaatttgt accaatcaat gacactggtg ctatcggtta cgatgccgtc tggtgttgga
     8701 atgtttttga tttgtaataa tttttttata gcagctttat tccagcaaag gaagaggata
     8761 tctgaaggtg aaccagtata tacaaattgt ttttcttcaa gaatttgcgc tacggtgtgt
     8821 tcgctatgat caacgccggg caacccttcg caccagttga agaggatata gtcattgggg
     8881 ttgtaagatt ttagtacttt gtctaaggaa tgatgagtaa ctggtacatc aacaacactg
     8941 tggccttcat tctgtaattc attaataagt atttgtgctg attgatgtgc tgcttcctgt
     9001 tcatgcaatt cccattgggg atcaatgtca tgaagcaaaa gaacaggaat atctcttcgt
     9061 cgttttgctg gttccatttc ctctcgtgtc tataaaaatt ttgaaaaaag gactatataa
     9121 attatatcga ttaaaattaa caaaataaaa atcattttaa tgttgtaaag caaaaaaata
     9181 tactatgaaa tgaattttaa aaaaattttt tattcagtga caaaatatgt ccctataaaa
     9241 tgagtatatt acaatcaaac agtattgaaa gggggatatt caatgaagcg caaatatttt
     9301 ctgtttaata aaaatgatat tttgatggtt atagttatag taacaaacgt tttatcattg
     9361 tgttatattg ttcaaaactt tttggtgatg atttttggtg tatttattac tggaatactc
     9421 tttttagggt gggtagctta taacaggctt acgcgatatc ataaatttat tggatataca
     9481 cgtaagctgg cagaaaaaac cttatggacc acaactgtta caataaattt aaatgatata
     9541 ccaaaatgcc catccaccag tatggagatt catcccaacg attaacatat aagcataatt
     9601 ttcttctgga ggtatttgta aatgctacat acctattatg acaatcccga tggtgaagta
     9661 tttatttata acaataatgc atattttttt aaatatcctt tcattgaaaa tgaaccagaa
     9721 cttatagaag aaaatgttgt gtactatact gagtataaaa tacctgatag tagatcatat
     9781 agaagtaaac aatatgatga tatacttgtt gtctataagg atggttcaat caatgggcaa
     9841 ataactggtt tgaactttta cttgaaagat gccttcaaac tatcagtata tgatagtagt
     9901 atactgtgta tagcaccaca taccatattg tatgttgata cagaacgtgg catacaattt
     9961 gaacgcaact ataaaggttt tattaccgat ggttggttag agtattgtga ctacaatgat
    10021 tatatacagt atctgctcat tgttaatggg aaaatagttt atggtaacaa cactatgaca
    10081 atgactatga accgtaagat agttacctat gaccaactgg taagcacctt tgaagatgga
    10141 tttgtgtata acaggacttt ttatgatatt ggtgctaccc caaaaaaagt attttgtttt
    10201 ggttcatatt gcttagcatt tattcatgat ggattatatt tttttgagtt tgttaacaac
    10261 agggcaaaga ctgcattttt aattgaaaac attgaggata tattttattc ctataaagaa
    10321 aaatttttct attactatga tacacatggt gaaatcaatc tatggaatcc aggtgattta
    10381 aaagaattag gttttttatc gtaatatcta aattattttt attgacaaat aaagttaaca
    10441 ctgttaaatt aacattgtta ttataataaa tggaaaagta caacatttag aatatccaaa
    10501 atgtcatcat aaactcagat tgaattggga tgcaggaact gtacgcagca gtaaattagg
    10561 ggggcagact cagaatgatg cttaattcat tacagattag gggaaacata atacatgcca
    10621 cgcacagcac gtaatgtaga agaggtagat attttcaaac agcagatact tgatgcagcg
    10681 gtaaaaatag ttatcaggga aggtttcgca aggcttagca tgagaaagat tgctgctgca
    10741 ttaggtgtta ctgccactac attgtataac tattacacaa acaaggacga attgtatttt
    10801 tatattcgca tacggggatt tgaattgctg tatggtttat ttgaaaaagc atatacctct
    10861 gcttccgata gcagggataa aataaaatct ctgattgctt catacattga atttggcata
    10921 cagtatcctg attattatga agttatgttt ttaaacagaa atgtgccaaa gtttcttgat
    10981 tgcattggga caccgttaga agtagttggc gcaaaagaaa aagaaatagc cttaaaaccg
    11041 tttgcgtttg ttatagagaa actacagcaa cattgtgagc ttgatccaga tagtgcatat
    11101 gttaccgcac ttcaattatg gatagaatgt aatggcgttg tgagtttata taacagcagg
    11161 cttatacgtg aggttcatga taacccgcaa cgttttgtcg atagttatgt tgatgtaatt
    11221 gcacagaggt atattcctct ggacatcaaa taatggaggt tcattatgtt acttttcaca
    11281 tcacagagca aattcaacac aattggtact ggtattttta taaaatcatt tctaaaattt
    11341 attgcattta attttaataa tcgcaaatca cttaatcggt accttaaaac aaaagatggc
    11401 tatctcaatt ttacaattgg gttgcgcacg gaagacaata gtattacctg cagcattgca
    11461 tttatggatg gcagggttac cgtttcagga actatcgccc cagatgctaa tgctgttttg
    11521 atttttaaaa atgaagatgc tgcacgcaag ctattacttg gtaccccaac tgatatcatc
    11581 tacatgctgt taaagagcga tgtccgtatt gatggcagta ttacctattc caatatattc
    11641 tcatttttta tttcacttct ttttaataat gttcagaaac gtgcaatgaa aaaagagcga
    11701 caggccgatg aaaaaaaagt taaaaaggaa catcctaaac cacacaaaga gctaagcgat
    11761 gaaattgcaa gaagaagaaa agagcgcctc aaagggaaaa aagaagatcc agatgtatta
    11821 tatctggaag atccttatct ttcacactat tgtcttgatg attttccgcg aatcaaaaaa
    11881 tttcttgatg accatttaac caaaaaacca gctatctgtg cggaaagagc aattcttttg
    11941 actcagtggt acagagaaca tggctttgat acggacaaca atggaacacc gtgggatcca
    12001 gtgttacgca atgcgtatgc atataaatat ttgatggaac ataaaaagcc tgtgatacaa
    12061 aaggattcac tgttgccagg aacaaccacc agcaaaccca ttggaagtcc cattttccct
    12121 gatagtacag gcatcatgat ctggaatgaa ttgctcacca ttccatacag aacatacaat
    12181 ccttttgata ttgatgacca gactcgtgaa atcttacatt ttgaaatact gccattctgg
    12241 ctgcaccgca attttaagga atgggtcagg gaaaaatata ataatccttt gtgtgagcag
    12301 cttgatgaac gctttgctct gtactttgac tggaagcagg ctactatctc acatactatt
    12361 ccaaattttc ccaaagttat ggctatgggg acatcaggaa tgatagatga aataaaagcc
    12421 gagcttgcta acgattgctc acaggaaaag cgcaatcttc ttacagctat gattgcctgc
    12481 cttgaaggta ttaatgcgta tgcaaaaaat ctttcacagc aggctctgag cgatagtaac
    12541 caggaaacag atccaatacg caaacaggaa ttgctgaaaa tagctgatat atgcagccgc
    12601 ataccagtca atccctgcat aacgctgcat gaagcgctca ctgctatgtg gattacgtgg
    12661 attgcgctgc acaatgaaag cttgaatgca ggcctttcat taggaagact tgatcagtgg
    12721 ctacagccat attttattaa tgatatgaaa aacactccgc cagagaagcg tgaagaatat
    12781 ataaaacagg caattgaact tgttgctgat ttttacatgc gctgcaccga tcatttccca
    12841 ttaacacctg atttagccaa cttctatttt ggtggtagct catccgatca ggctataaca
    12901 ttaggtggtg ttacaccaga tggcagggac gcagttaacg atatgacgta tattttctta
    12961 aaagttactg aaatgctttc tattcgtgat cccaacgtca atgcccgcta tatgccaggt
    13021 gtaaacagtg atacctattt aaagcgcctt tgcgaagtaa accttattac tgcagcaact
    13081 ccatccatgc acaatgatgc tgcaatcatc aaggctctga caccactggg atacgatata
    13141 caggatatac gtgattggtc tgcaacaggc tgtgttgagc ccacactgtc gcataagcac
    13201 tttgggcaca ccaatatgca gatgatgaat atggtagcag ctcttgaaat ggcacttaat
    13261 aatggctatc atccgttaac gcggctggat attggaccaa agacaggaag gcctgaagac
    13321 ggcgttttta aaacttttga tgatttcttt gaagccttta ccagacagtt tgcctttatt
    13381 attgatcagt ccattgatta taataatacg ttagccgaag ctcatcagta tttgcggcct
    13441 acaccgctgc tgtcagcatt tattgaaggc tgtttagaaa aaggcaggga tgtaacaaag
    13501 ggtggggcaa agtataatag ctcaggtact gcaattattg gccttgctga tgtaacagat
    13561 tctatgatgg ttgtaaaaaa gcttgtttat gatgaaaaga agtacacctt tgctgatata
    13621 aagaaagcaa ttgataccaa ttttcagaat gatccagtat tgcttgcaac agttcataaa
    13681 aaagttccat tgtttggttc aggtaatgaa gaagctgtag caatggcaaa caggatagct
    13741 aaatgggcac atgattactt tgcaagtatt ccacattacc gcggtggtac atatactact
    13801 ggattctggt caatgtcaaa tcatgtcgca tttggcacac ttaccggcgc actgccttcg
    13861 ggaaggttag cgggaaaacc gtttacacca ggcttaacgc ctcagccatt tgcttcaaaa
    13921 aatctattgg ataatatccg ggatgttgca aaactggatc cggttaatct tactaataat
    13981 attgcattta atgtcaaggt ggtgccttct gcaatagaat cgcgggaaaa gactgttcat
    14041 gatatgttct cttatgtgaa gacctatttc acgttgggtg ggatgcagat gcagatgaat
    14101 gtggttacat cagcagtatt gcgcgatgca atggctcatc ccgaaaacta tcgcaatctt
    14161 attgttcgta tttcaggata caatgcgtat tttgttacat taaaccgtca catgcaactg
    14221 gaactgattg aacgcgctga atatggaata taaactttag gatataaagc aaatgaaaac
    14281 attaatactg gaaattaaag gtaactcact tgatgatggt ccgggcatac gaacggttgt
    14341 gttttttaaa ggctgtccgc taaactgtgt atggtgtcat aatccggaaa gtaaaaaagc
    14401 tgtcattgaa ttatcctggg acaaaaaaga atgtattgct tgcctgagct gtgcaaaggt
    14461 atgtcctgaa catgcaatcg atttgaaata tcaaaacttt gtagatagaa aaaaatgcac
    14521 gctgtgtttc aaatgtgtcg atgtatgtcc atcaggtgct atgagtaccg tgggaaagcc
    14581 aatgactgtt gatgatatca tgcagcatgt gttaaaggat aaaccttttt ttgatacatc
    14641 gggtggcggt gtgacgcttt caggtggtga gccgctttta aacattccat ttgtatcagc
    14701 attattgaaa caatgcagga ataatgcaat tcataccctt atagaaacaa gcggatattt
    14761 caactggaat gaatttgaaa gctgtattgc accatattgt aacacagttt actatgatat
    14821 taaattaatt gatgaagaaa aacataaaat gtactgtggt gtttcaaata caaagatatt
    14881 gtcaaatttc aagaagctta tacagctatc gcaaagtatg ggaatacaga ttgtaccacg
    14941 tatacccctt gtgccaggaa ttacagcaac ggaacaaaat cttactggca ttgcaaaatt
    15001 tttgcatgac tgtggtgttg ataatgttga gctcttacgg tataatccat tatggcctga
    15061 gaaatgcatg aagattggaa ataacgcaaa agtagatgaa gcattaacca gatggatgcc
    15121 tcatgaagaa tacgaacgct gtaaaaagtt ggtaagtgga gtaaccatta cattgtaaaa
    15181 gttcgcatat tataattata tctcttcaaa tttatgtgtc ttgagcgaac gataagcagc
    15241 ctcaatcaat gctaactctt tacgtatacg taaataatca ggttcccagg gttgttgtgt
    15301 gcgatagtta tcaataaaag atctgtgcat agcaccaaaa cccaaaaaat cactgaaaga
    15361 aaaacgtaaa gcaaatcgat tgccattcac aaaggtaaag agcccattgc tttcaaaggt
    15421 gatattccct tcggtgccat agattttaga taaggccatt cctttaaatg tattggaaac
    15481 aaaccatgaa tgaaacaatg tgccaaaggt gccattgtca aatttgacag tgatatggat
    15541 agtatcttca aaaggtatgt ttgtttggta ggccacgtca gctttgatag cctgaacggc
    15601 aacaggtgtt gcacctgcaa gtgatacaag ctcattgacc cagtggcaac caccttcaag
    15661 caatgcacca ccacccatta gtgctgcatc agtgcgccat cctgtaatag tatcgtgatt
    15721 gtgtttcgtt agccgtatat acagaggttt gccaattgag ccattagcta tatgcttatg
    15781 tataattttt agaaaaggct tgaagtaata attttcagct actgtaaaac gaacattgtt
    15841 tttagtaaca gcttttacaa tagcatcaaa ttctttgaga ttgcgtgttg ctggcttttc
    15901 aacaatgaca tgctttttat aattagctgc taaaattgct aagtctttat gatatgcgtg
    15961 gggagtggtg atgaatgcaa tgtcacaatc ggattgtgct gcttcttcat agtttttaaa
    16021 tgctatgcca ttaaattgtg ctgcatattc ctttgcatct ttttcataca ggcttgaaaa
    16081 acccagacga ataccaggat acaacttttg tataattttt gcatgacgct ttgctatata
    16141 tccgcatcca aaaaaacata taaatggcag attatcctgt ttcatagcat caccttttta
    16201 gcatgatgtt tgctacgcgc aacgcatttg ctgcaatggt taaactggga ttaaccgagc
    16261 caccagtggg tagcacactt gcatcagtaa tatagcagtt tgtaatatcc cataaacggc
    16321 actcagggtt aactgctgaa aggttgcggt tgttgcccat tctgcaggtg cccagtgcat
    16381 gggaaaatgt tactattggc atatagtaaa aaatttttgc gcctgcctgc ttcagtatgc
    16441 gttttgctac gttgtacagt gcatcgcgaa cggcaatatc gcgtgcatga tacctgtgat
    16501 aaattgcaac attgggcatg ccaaatgaat ctctcgtatc cgcacaatac acgcgattgt
    16561 catattgtgg aatatcttcg gcaatgacta attggttaat aagaaattgt gaaaacgttg
    16621 cagcaatgtt ttttaatcca aacggtgcat ttgcttttat aacgccttta cccggcgtgg
    16681 ctatgtcctg aataatgccc cagttgcctt ttggggagct tttgccggaa ctatcgccaa
    16741 aataatagtc ggcaatagca acccgcttgg ccagtgtgtt atcagggttt gctatatagg
    16801 ggacaacacc gcttaccact ccgttaatat gacgcatcag gttatgacca atatgctggg
    16861 ttgcagaact aacgctttca acccctgaag caagtaacag gtgaggtgta gcgataggcc
    16921 ctgcagctat tatgagatat ttacatttta tggtatggcg ctgccctgtt gcctgattaa
    16981 taatgtcaac cgactgtgct ttgtttttga taaaattaat ttttactgca cgatgattat
    17041 caagtacaat agcaccatgc ttgattgctt ttggcaaaac cgaaacggat aagtccatct
    17101 tggcctgaat tttgcacaga taatggtcgc aggtgttaca cagaatgcat ggtgtgttat
    17161 tacatttgcc agtgaaatta atagccattg gtatgtaaaa tggatgcagt cccagctgtg
    17221 ttgccgcatc ccatatcttt tgcgaaggac gtgataattg cagaggtggt ttttgtggat
    17281 aatctttttt tcgtggtggt tcggtaatgt cctgcccacg tatcccagca acacactgaa
    17341 gttcttcggc tttgtcataa aaaggtgaaa gtgtggaata gccaaatggc catgccagtt
    17401 cacttttgcg tttatcggta tcgggttgtg gaggcccttc aaaatcctga actctgagcc
    17461 tgaacgctgc agcaccgtaa aacgttgaca tgcctccaac cgtatcgtac ggccagtcag
    17521 cttcaatttt tccctgctgg tcaactaaat atggcgtata tccctgatac agtggttttt
    17581 tctgaagatg caatgcatat tcgtcccagc aggtatcatc acgcttcacc cacacccctc
    17641 gttctacaat gcatacttct tttcctgctt ttgctaactc gtatgcagga aatgagccgc
    17701 caaagccgct gccaattatt acatattcgt acgaagattt aattgttttc atgatggatt
    17761 attgtgcagt aactatcgct ctaagaaata aaaggccata cgcccaactg gtacatgcgt
    17821 acaaaaagga aaacaaatgc accgccttca agtatccatg tccatgcctg tacataggtg
    17881 ccaataaggg cgtcccagcc tgaatgaccg gtatcagccc acaggttaat ggtaaagcgt
    17941 ttattggtaa atggcataag cagtggtaac ccaacactgc ttggcatgtc agctaaatag
    18001 tgtataatca ggctaacgaa agcaagtgct gcccatgcac tgttaaatgg taacaatatc
    18061 agagcaactg caaatgcaaa aattagtgaa tgtgaccatg tacagtgaaa agaatctttt
    18121 gcccatccta atttaattgg gatgacatcc agattgggaa gccagtgagc accaaacgca
    18181 accagaatgc tttcaagtga aaatgcctgc atggcaccac cggttatctg tggtgcgtat
    18241 gatgatatga taaatgatac agcaacatga cctaattgca ttgttacctc cgaaagataa
    18301 aaaaatggta tggtttaatt atacatacat tattgtactt ttaattcgat gcaatcatga
    18361 agttctttta ttgtatataa aaatgctctg gtatctgata gatcctgaaa aaagtaatca
    18421 ggtttgtgct gtagcagtgt ttgtgcatct gtccaacccg ttgcaacagc aaccgttatt
    18481 gcaccagatg ttttgccgca ttcaatgtcg tagatggtat caccaatgat aatcagatcc
    18541 tctggcttaa atgttcttcc cgtaagcgag tacaaccgtt gtagcgcaat aaaaggcatt
    18601 tcattgcgaa tatgggtgtc atcggcaaat acacccatgc tgaaatactt aaagatatca
    18661 aagtgctgta acttaatcaa agcaccctga tagaagttac cggttaaaag acctacaatt
    18721 acattaccaa attcagtaag tgctttcaat aactcatgaa ttcccggcaa aaggattgct
    18781 tctgaatgtg gcattatatt ttttaagtat gtaaaatacc gttgcttgaa atcttctata
    18841 taggcaaaga tagtttcttc cgtaatgcca tggggtatca gcgattgcat gagtatgaga
    18901 ggatcagtgc gcccctggaa attaactaat tccatatcac cggctgtacc atatacttca
    18961 atggcagcct gaaccattgc ctgcttaccg gcaccatgtg ttctaagcag cgttccgtct
    19021 atgtcaaaaa gtatagcttt tatgactttt tctcctcgta aaagttaatt tttaaaaagc
    19081 gttcttttgc agcacggggt gcagggtcaa aaagcatagc aatacccagg ccaggaagga
    19141 gcgaaagagt atagagccat attgccagac gtatggtgaa aggtcgtccg ggtatttcaa
    19201 aaatcagaac aattaatatt gctataatca ttgcaaaaaa catagcaagt acaggtgttc
    19261 gtggtagcat ggttaatccc tttttaataa gatagtattt taagaatatc aataaacaag
    19321 taatagcaca taaaatcaat aaaaaaagtt atttggcata actatcatga catttcctta
    19381 tgattgtgaa agtgtatgat caagctgatt tgaaagatta actaaatata actaatcctc
    19441 aaattttttt gccagtgaca tttagattcc ccccttatct taaataatca atatagaata
    19501 gattaatcgt atagcaaatt ttacaaaatg taaataaaaa aagtttgact tttttacatc
    19561 atatcataat atgtacttaa agtctatatt aatctggaat atgtaaaatt atgcctgcac
    19621 atacaatcaa atcaaaaacc cttttaactg tattcaaagg gattattgca caggatgatg
    19681 taaatgaaaa tgatgtttat cagcaaattc aaagctattt aactctgacg cctttctatg
    19741 ttaaattgtt tatgtttttc agtttagtat attgtaatct gctctctgtt gtaactaatc
    19801 aaaaaatgat atattcactt cccgggaata aagttgctgt tcttttgaat aattgtgaaa
    19861 agcattttat aggtagaagt attatcatgt tgctaaagct tatctgtacg ctggtatact
    19921 ttgacgatga caaacatgca gagcgtattg ggtacaggca tttagttcat tgtaaatagg
    19981 taaaagatat gatatataca ttaaaagatt acaaaaagaa ttataaaaat atttttgatg
    20041 ctgatgtatg tattattggc agtggagctg gtggtgcagt tgcagcctat gaacttgctg
    20101 caataggata tagtgtcgta gtagttgaag agggtggata tttcacaaca aaagattatg
    20161 acggaaaacc tctaaacaat atgaaaacca tgtggcggca agctggcagt actgtatcat
    20221 ttgggatccc accaatttca atccctacag ggaaatgtgt tggtggtact acagcaatta
    20281 actctgcaac atgttttcgt acaccagaac atattatcaa gcagtggaaa gaaaatttca
    20341 aggttgatat agattacaaa agtttagaac catactttga aagggttgaa aatattatta
    20401 atgtaacaga attaccctgg gatgtcctgg ggaactgtgc aaagattgta aaacgcggtg
    20461 ctgatgcatt gggattgcat tgtaaaccgt taaaacataa tgttaaaaat tgcaaaggat
    20521 gtggtacatg ccagtttgga tgtgctgaag gagcaaaaca gagtgcagag ataagttata
    20581 ttcctttagc attacaatac aatacgtcct tacttacgca ttgcagggct gaaaagatac
    20641 atttcaaggg aatgaaagca gattctgttg aatgcagggt ttataatgaa aaaggagaaa
    20701 gtgtggccag ttgcacaatt aaagcaaaga ttattgtctg tgcatgtggg acaatgatta
    20761 cacccttatt attaaaaaaa agtggcatta aaaacaaaca tcttgcaaaa caccttcaga
    20821 tacatccatg ttctcgtgtt gttgctctca tggaagaaga agttaacggc tgggttggtg
    20881 tttcacaggg tgcatatatt gatgattatg cagatgaagg gataatgctg gaaggcattt
    20941 ttgttcatcc atcaatattg gcagcatcac tgccaggagt tggtaaggaa ttttttgagc
    21001 tgacaaagca attttcgcat attgctgcat ttggtgtcat ggtacatgac acatctgaag
    21061 gggctgtctt tgggaatatg catacgtcgt tttcctggta tatgatgaat acaaaagata
    21121 tacaaacatt gaaaaatgga atagcttatt tagcacaaat ttttatggct gctggagcaa
    21181 agaaaatttt tacacctatt tcaacatttc ctgttatttc ttgccaggat gacatagaaa
    21241 agcttaaaca aacatattta atgcgaagcc agatccctga agtttttgcg tttcatccat
    21301 tgggtacatg tcgtatgggt ggcgatagtg actgtggagt agttaatagc tacggcaaat
    21361 gtttcaacac tgacaatctg tatgttgccg atggcagcat tgtaccaaca tcacttggag
    21421 taaatccaca gataacaata atggcattat caaccagaat tgccgggcat attgcagaac
    21481 aattaaataa ctgaaggaat tgcagtatga ataggattaa aattatatgt gtacttgtga
    21541 tactggtttt tggtctattg gttttgtatc acaatgctta cgctgatgtt gataattctc
    21601 tattaaaaca ggcagatata caatcactac gtgaaggtca tattgttata aaaaagcaaa
    21661 aatccaaaac acaggaaggt gaaattgctc gtgtagctgg tgcaatactt atacaaaaac
    21721 caattcagga agtatggaag tgcattcttg attgggaacg tatgcctcag tatgttgata
    21781 cgctggatta ttatacagta ctggcaaaga taaatcacaa aacctcaatc attggaggac
    21841 agatacatgt tgcttttttg aagtttaact atacattgtt agtaagtaat gatgaacgta
    21901 attattacca aaaatggcaa ctgatatctg aaaacgatat tgttcaatat aacatacaaa
    21961 aaaatatcaa acctgcgagc accggtataa aaaatataga aggatatcag tattgcattc
    22021 cacttgatga taacaatact atagtatatt atgcaccagt tgttgaagta tcagtaccgg
    22081 taccaggatt tgttgaaaac aaccttacac aaaagagtat taaagattat ctatatggta
    22141 ttaaaaggta tttagaaggg gggcacaatg aaaaaaaata gaatcattca aataattact
    22201 ataacaggat tattatatgc aattgcattt attattacaa caattatgtt tatattcttt
    22261 aattctacac tgataaatac tattaatgtt ttatcacaaa aactgattcc agcacttccg
    22321 ttagcgcagg aacacagcca attttttttg attttaagtg tatccatgat gtcaggagta
    22381 acagtatgtt cgcttttatt gtataaaaat gctgagcttt atattgaaat ggctatccca
    22441 ctcataacca tgaaatttac ttcgtcagtt tttggtttac ttttctttgt gtatgggtgt
    22501 atatatcata atggctggaa caccatggct aatctaatta tatttacaac ggattttccg
    22561 ttaggattgt gggttctgta tgtatatagg ttattcaaac aacaaaaatt ataagaggga
    22621 tatatgaata aagaagaact tattgcttca gccaattata cacgctatat cccaggaaag
    22681 agtgggcatt atgaaagttt ttttgtcaga gcaaatcatc cacataagcc tctggccttc
    22741 tggattcgtt atactatatt ccagccaaaa ggaaaacctg aaaatgccat tggagagtta
    22801 tgggcaatca tttttaatga ggaaaatgat aaacatatag cgttaaaagc ggaatatccc
    22861 atttctcatt gtagtttttc aaaagatgat ttaaatattg ttattggtga tgctgtgttg
    22921 acacctggag ctttgcaggg aactatcacc tataataata gtcaaaaaat acaatgggac
    22981 cttacattta caggcaatga agaatcagtg tttttgttcc cagtgaagct gtatacaaca
    23041 aaattaccaa aggccaaatc attagttgga ttgccaaacg ctttattcaa tggcatgatt
    23101 aatgtaaatg gggcgataat tacagttagt aactgggttg gcagccagaa ccacaactgg
    23161 ggattaaagc atactgacca ttatgcatgg ggacaggttt gtggatttga caatgagcca
    23221 gatgcatttc ttgaaatagc aactgcacga ataaaattgg gaccactatg gacaccgttt
    23281 atgactccaa ttgttttacg atataaaggc aaagagtata ctttgaattc actgggtgaa
    23341 acattctgca gagcccattt tggctatttc tactgggatt tcaatgccag atcaaaggac
    23401 attagcctca caggttcaat atttgcactt aacaaggatt ttgtatgttt gccatattat
    23461 aatccacctg gtggaataaa atattgcctt aatacaaaga ttgcttc
//
LOCUS       DAORYL010000045        23470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1827_length_23470_cov_11.784753, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000045 DAORYL010000000
VERSION     DAORYL010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 23470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23470
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1827_length_23470_cov_11.784753"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..1293
                     /locus_tag="PLV81_09245"
     CDS             88..1293
                     /locus_tag="PLV81_09245"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOM10320.1"
                     /translation="MSVKKVVLAYSGGLDTSVIVKWLLETYKCEVICYAADVGQEEEL
                     DGLEQKAINTGASKCYIEDLKEEFVRDFIFPCIKANAIYENRYLLGTSIARPVIAKRQ
                     VEIALKEGADAVCHGATGKGNDQVRFELTFKALAPQLKIIAPWREWNLTSRSSLFDYA
                     EKHNIPVPVTKDKPYSMDRNILHISYEGGILEDPWREPDESMFILTKSPEKAPDIPAY
                     IEIDFEQGVPVAIDGKRMDPVSLMKTLNKIAGENGVGRIDIVENRLVGIKSRGVYETP
                     AGTVLHVAHRDLEAITLDRDTQHLKDRLAIEYAELVYYGQWFTKRREALDAFINETQK
                     NVTGTVRVKLFKGTCMAVGRKSPVTLYESDIATFDESALYDHRDATGFINLFGLQTKI
                     EALLKQKRK"
     gene            1295..2227
                     /locus_tag="PLV81_09250"
     CDS             1295..2227
                     /locus_tag="PLV81_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10321.1"
                     /translation="MSDSTFQKLEQSYPNRIQNHALFNDAWYLFVNDYPPTHCLLDFK
                     ELPSTRVALHAGDLGFALLWIVQSVLIRCIIYNINQLQPWDIQLHTTTIGAMAHTEPD
                     TNRMIAQYSDSNTLVISGKKNYISGGSDADILLLTARRPGEDKFTKMMYIPVKSLPEN
                     ALQDINLSILPTIRHSSLTLTDFACNASQCCPVKDKELRKILKKWSLIERSLIGEAYI
                     AFLCYLAKNIPLPDQDQTLNEAEELLSLQKNFSREQLENAFSDKYVDTLGDVARVLNI
                     TQKIANYCMENTNSLTQPIQLRINDLNLFNKIRL"
     gene            2379..3287
                     /locus_tag="PLV81_09255"
     CDS             2379..3287
                     /locus_tag="PLV81_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOM10322.1"
                     /translation="MFSSIEETIQQLEKQKYICSKEIATTVYLALKMQKPVLVEGPAG
                     VGKTDLGKCIAQALGYELIRLQCYEGLDEAKALYEWEYAKQLLYTQILKDKLSKILED
                     QATLKDAVNKLSTEEDVFFSEKFLVARPILKAITNETPSVLLIDEIDKSDSEFEAFLL
                     EVLSDFQVTIPEIGTITANNKPFVLLTSNNAREMSDALKRRCLHLYIDYPTKELEEQI
                     VKLKVPECGNYLIKEIINTIHEIRKLPLKKDPSISETLDWAKALALLGCEVLNRDIAI
                     DTLNFILKYEKDIELVKKNINTIIVH"
     gene            3291..4664
                     /locus_tag="PLV81_09260"
     CDS             3291..4664
                     /locus_tag="PLV81_09260"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOM10323.1"
                     /translation="MIYDKAFINEEDKAKGVFFEFAEVLKKAGVNLSIVEVMDMMHAL
                     PLISIAEKEIFKQTLATTLIKDYTDIPIFNQCFEEFFQRKKGNDSLLEVYASHTNTDD
                     FIFSQEEKSILESLIDDFLDSHPQSFFKKSNEYLTKIFINEIQSSEAGGAIGLSLFNR
                     RSRFASGGAEDSIQQELSPQVFEAIMGMLRRRMFERNMGSAIKQREDYLLNKYIYQLK
                     PDEIKEMRELIKRFGQKMKNRISLRKKKVKRGTIDIKRTLRRNLQYGGVPFKIFHRDR
                     KVDRPQLVVMCDISSSVNQYSRFMLLLTYTLQSLFAKVRTFAFISNLVEITDLFREMD
                     PERALNSIFNDTNFTYGWGSNYGNSFNQFIQNYSDALTSKTTVLILGDARNNNQDPGL
                     DSFIKIKERSRNIYWLNPDRKHLWDWSDSIASLYIPHCTEMREVKNFLDLSHFIDTLF
                     IQKRNYK"
     gene            4830..5900
                     /locus_tag="PLV81_09265"
     CDS             4830..5900
                     /locus_tag="PLV81_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM10324.1"
                     /translation="MANTIKILIIDDEDIILKSCEKILKRREDLEIDTAQNGFEGLEK
                     ARSKKYDIVITDLKMPQLGGMEVLKTLRKEQPDVTVIIFTGFATVDTAREALKLGAFD
                     YIPKPFTPDELRNVVDNAINARQNKAEAAMLDLMAIVSHELKSPVSVVHTTAETLYKG
                     YFGNLSPEQQKIIETILRNCQYLEDIIRNYLDLSKMELDSLESFQQEIDLYEDVIKPI
                     VEMPEHANNIKNMKLNLTIEVKPKIKADANLLKIVVTNFVNNAIKYGKADTTINIELK
                     QIHNEYVFSVFNEGVGIPPEGMDKLFKKFSRLKQKGTEGIKGSGLGLYICKSIIEKHK
                     GRIWAESEYGSWVKFSFSLPVS"
     gene            5983..6306
                     /locus_tag="PLV81_09270"
     CDS             5983..6306
                     /locus_tag="PLV81_09270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOM10325.1"
                     /translation="MEIEFKDIGEHKVISISGEVDLYNVSELKKALFSVTDGSYSSVV
                     VDLKDVNYMDSSGIGALVAGQKKMRAHNGKFALMNIHDDVLNILKLATLDKFFTIYES
                     EEDLL"
     gene            complement(6316..7908)
                     /locus_tag="PLV81_09275"
     CDS             complement(6316..7908)
                     /locus_tag="PLV81_09275"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOM10326.1"
                     /translation="MAEEKKIKKEKKESKENKGKNNKDEQKVTSSKEVRFSLRLKFSL
                     AIIVLASSIIAVMTYYFIQQESELLRKQILQFANRETERLAVIARESISQKDELPLVG
                     AVQDLKNLEFVNYIYVLDNNKRIIQNFDPEKNGTIIDDDLTKRAIDNTNTEKPMLVIY
                     KDAKTGINFYDLSLPLFHTVQKVRIGTVRLGFSEAIILSEIRKMTITILLIGALFIGL
                     SVIGSIVLSSIIIKPIKKLTEGAVIIGNGNLDYKIELDSTDEIGKLASEFNQMTERLK
                     VAQKMEIESRIMQEQIDLAREIQEGLNPMGFYNKKGIEIKGFTRAAKGVGGDYFDYID
                     IDQYRVGALISDVSGKGVPASLVMVMIRTVFVSYIRKGDVQCADVVKAINDSLSADFA
                     VDKFATLFFMIYDRSTHELSFSNAGHGPLFCYRSALDACTLAKLDGVPIGIMEDVDYK
                     QAKVPLNVGDIVVLYTDGVTEMRNANKEEYGQKRLKDLIVSNKHLNAKELADLIVQDV
                     DNFRGEVPPHDDMTLLVLKRTA"
     gene            complement(7912..9879)
                     /locus_tag="PLV81_09280"
     CDS             complement(7912..9879)
                     /locus_tag="PLV81_09280"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM10327.1"
                     /translation="MKIRKNITYIVLQYLCTSCQRIGVILFIILFSQLMTTVAISQNP
                     NTIAEINKRAQEYYDKKELSKAIIEWLKILEIDPNNEEVQRKIELVYEEKHKKDISLQ
                     RAKLYYRLSRKVLPDNVENAKNNSKIAIENFIIAYRMDPNDPELQVLREDLKLLDNEI
                     KLELAKKRMAEEIKKKYYVLLEDANKLMLEKQYEDAIKIWDEILRLVPLDTVALEGKR
                     QAELAISNRLKYEKIMMLIASGEELFKQEKYIDSRNEFLQVLNLDAKNRVAKDYISKI
                     DDILEEKRTYEQRRIQAEQFYVAGINNLKTYEFDQAKDNFENALALIDNYKDAKAQLE
                     AIPRLKKEYEEQQRLLKLQKIDKTFQEGLLALTEGRYRDAIAAFQATLTLDPKNELAK
                     RYLSTALDALRVQEEEVVDENNPYYSIVQPLIESGKRLYNQGNYDESRKQWQKILNLF
                     PQNRIATEYLLRCDLAQNPESFKKFADNIVNEGKKYLADRKFNEAYRKFELIASIDPQ
                     YPEIQNLMALSRVEKKKAGFEGTDEDFRRRYNVAMQLYQQGGKNNLQKALAEFRVINN
                     KYPEYIQVAIIINKIESQLRFEVTEEREIKKLTPRQQELVREYYFKGINYYSNNQFEL
                     AITEWRKVLAIDPTHEKAKNNIKKCLILLGR"
     gene            complement(9886..10332)
                     /locus_tag="PLV81_09285"
     CDS             complement(9886..10332)
                     /locus_tag="PLV81_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10328.1"
                     /translation="MNKILKQAFIVLLIQSILFISCSKKKDYSDIDNLLTQWAYAIRN
                     LNYSEYSRLEAYPKDINTFNAMYQQYYIANIKIVKVDEKLHHGTDNDGKPYEFKNVQF
                     IASLYNRQTGREQQIVNGDIKLIRYISGSKKNRGWLLSNRTFIYQE"
     gene            complement(10398..10973)
                     /gene="amrA"
                     /locus_tag="PLV81_09290"
     CDS             complement(10398..10973)
                     /gene="amrA"
                     /locus_tag="PLV81_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOM10329.1"
                     /translation="MVQLNDEQKKKLLALARRTIEEYLKKRTLPKVDYNHEFADDVFK
                     QKCGAFVTLHKYGALRGCIGYVKGIKTIPEAVVDMAKASAFEDPRFEPLRKDELDDID
                     IEISVLTPVEPVNDIGEIVIGRDGLIISNGFRAGLLLPQVATEYGWDVQTFLEHTCYK
                     AGLPADAWKWENTKIEKFSAIVFGEKELNLR"
     gene            complement(10983..11801)
                     /gene="amrB"
                     /locus_tag="PLV81_09295"
     CDS             complement(10983..11801)
                     /gene="amrB"
                     /locus_tag="PLV81_09295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOM10330.1"
                     /translation="MWQRKPGFAGSFYPASPEKLKNEIITYLSKATVKKLEHPVAFIV
                     PHAGYIYSGPVAAYSYAQLKENPADVVIVLAPSHRARFNGASIITNGIFSTPLGDITV
                     DSTIAERLLDEVVFFEIKEAHQYEHSLEVQLPFLQVVLKDFVLVPIVIGTIDLELCRQ
                     IGKTLAHVLQDEKRNYSIIISTDLSHYYHYDIARKIDTTFIESLKKFDENYLYEMLQS
                     DKAQACGEGPVLAGMVASKQLGAQRIEILQYANSGDTAGPKNEVVGYLAAAFVK"
     gene            complement(11963..13873)
                     /locus_tag="PLV81_09300"
     CDS             complement(11963..13873)
                     /locus_tag="PLV81_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOM10331.1"
                     /translation="MKRLVLCFVAILLLYIPVFAQEGENVIQLDDVIVSDTVLYPGAY
                     YSISGSTVKDSVNTDLISVIKSQVPSLYTGSNRVMGYGVARSGAATISIRGIGRSGWG
                     PTTGIPILYNGMDTTMGIMGHPVADIMTMKNVERIEVLIGPQPVLFGSSAMGGVINVI
                     TKKQKEDGFTTELQGSYGTWNTTEDYLYHMGKFGNIDYSVGYQFQKTDGDWKQTINGK
                     TYTSEFMEHNGTVHVGYTLSKNWYASVDSYGMKQDINDPGPEGMPAGQIGLLEEFDVT
                     RGGVVAKLTHDYGTYSGSLQAEGNFGRHESTQESTGDDKFYSSDSSYAVRLKEVAKLW
                     QGNTLTAGAEWRRYGGTAKDRSSGTYYLDDKFMYDTSVYALMDQNLFKDVLSINGGAR
                     YSYNSEYGDYTAWQAGAAVRPLEKTKMYTNVAKGFKFPDIRQVYLQGMFPAINPNPDL
                     KPETYTSAEIGLEQQIIEKINIHVAGYRIWSKDKFVYTTQWINAPDFKYNGAEASVTV
                     QALTWLAFNAGYSYIDNEQDDTYLPYVPKHKANAGLTVSYKDFKAGIDGEYVKGLYAD
                     TAGVKEFDSYLVANASLSYTILEKYTAFVNFYNITDKDYATFAVSNPTQTLFWEYPMP
                     GFHFLAGLKATF"
     gene            complement(13981..14367)
                     /locus_tag="PLV81_09305"
     CDS             complement(13981..14367)
                     /locus_tag="PLV81_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10332.1"
                     /translation="MNKNIIYIVLLICAIVHSIGCSAKKDTQQTIKLVVVSAYNCDSC
                     SAIDDTIEYLQRIDRALEIKKVDIHNPEGAMYVSQYHLWRIPVYLFLDGNGMELYRLE
                     GEQSRSEIEKGLSIAKQRITNRNKPE"
     gene            complement(14377..15477)
                     /locus_tag="PLV81_09310"
     CDS             complement(14377..15477)
                     /locus_tag="PLV81_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10333.1"
                     /translation="MKIKGSHIAILLVIIIIVIIMLISKSENNNQPSTLSAGDSLKGY
                     GTVLEQMGLISPQQALQDYKQLAIYPPNSRPLSKENIDLLMPNRRYEQPLPIEKDSDI
                     YYLFTADKYRVIGDEPITFVLMVLQGTKPDSDRVPVTITKSIIKKGIDIKTAKYVSEL
                     SLIKNNVGDYTADVNCAQVFTSDKKSGTYWAQVEFKAADEVIDAAIPFEYFPENTIPA
                     KFTGNFRDYIKNGSFYIDVEIDVLRKGYYIIDANLFDNELEPVAYTIEKRELQAGRQW
                     LPLLFFGKVLLDKKAKPPFVLRDLRGYRFIEATTPDPEFMDREMMKPYDGEYKTKVSS
                     LQGLSDKEWDDEQKRKRIEFLEKESMGDIGVQ"
     gene            complement(15674..16396)
                     /locus_tag="PLV81_09315"
     CDS             complement(15674..16396)
                     /locus_tag="PLV81_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10334.1"
                     /translation="MKRFAVVLLFVAGFVFIASAGFSTTYILHLNGMCSTNWLDGKGT
                     SRLASKSGYTSINCYVDNRNTIAYSASQFKTNYLDVYCKGSNWCYIVNYSAGDVIMGY
                     INANYTPQWNIAYVYTTAGAGGGSEIAIKGISDLFTCNLAGQLGVSTVRNMYNHNDTN
                     GITIYHIGGYKGIFGASVLLPGEDDGAVAYHSSGACVNSGSYNSLCTCTHWTNHVIAY
                     TCTGYYLDHYGMKMKFIYQLGW"
     gene            16598..18106
                     /gene="amrB"
                     /locus_tag="PLV81_09320"
     CDS             16598..18106
                     /gene="amrB"
                     /locus_tag="PLV81_09320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOM10335.1"
                     /translation="MNGIPVIAVLILLFSLQCTESYSITKQSKLAGSWYPSDKNELLK
                     YVSSSEHPSQQISHIPLIIVPHAGYIYSAHVAAKAYSAIASYKPDIIVIIGPSHYEYF
                     TGFALPVYDAIKTPLGVVTVEQKVIHTLSQNNTFTFNNNAFDPEHSIEIQLPFIQHYF
                     SGIPILPVLAGNVTIQQARQAAMQLLPAIAHYKKPLFVISSDFTHHGQRFSYAPYYKL
                     QPDKRMEDIKRDDMKAIDYILSKNTEKFYNYCQSTGITICGHNAIVLGLLLLSKVYTK
                     ELVAYSTSASITNDYDNTVSYAAVVITGEINTISTSTLGDSSCNISSEDKKFLLSLAR
                     QSIETMLKNKTLVEYKGTIPEHCKQNAGAFVTLTINGNLRGCIGYIDPVKPLYQTVIE
                     CAAHAAFEDPRFKPLTKDEYKKIHIEISVLSPYKPIASLDELRLGTDGLIVVKGNRRG
                     VLLPQVATEYCLTKQQFFQHTCEKAGIYPFEYDSVTMYTFTAAIFSENEIMK"
     gene            18167..19288
                     /gene="amrS"
                     /locus_tag="PLV81_09325"
     CDS             18167..19288
                     /gene="amrS"
                     /locus_tag="PLV81_09325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOM10336.1"
                     /translation="MRSVTRKQFLHSCLYSMQALVVGSVIPFLPEDVIASNYRQALHW
                     KKIDAQTVQCNLCPNQCMLTNGTRGLCKARQCMDGKLYSLVYGRIAAYHIDPIEKKPL
                     YHFLPGNDALSVATAGCNFSCKFCQNWQLSQSQPEKLDSQYITPVQLVKQAKEHKTPV
                     IAFTYNEPSIQYEYIYDTVQIAKKDNISCVIISNGYLNKHASEMLYPLLNAIKIDFKA
                     YSQKFYSEICGGNLSPVLSNIELAKKKNVWLELVNLVIPTLNDNPDDIGAMVRFIKTH
                     CGRTTPLHFTRFYPIYKLKNIPPTPVSTLERCREIALAEGLKYVYIGNVPGHSANNTY
                     CHNCGKPVIERSGFYTTKNKLKSGKCPYCNTAIPGIWTL"
     gene            19285..21465
                     /locus_tag="PLV81_09330"
     CDS             19285..21465
                     /locus_tag="PLV81_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10337.1"
                     /translation="MKYNRVFLYISTFFIGISGSIAQAIMIRELLMFFRGNELSIGII
                     MVLWFAGITAGAAISKKIKAHQFSSIAHNILLHPSSVCISLILLYCTPIFYHTYDSFY
                     PLEVELIIAVVCLLPQCFIIGYIFPVLVNACSRLTINNPGTVVFLLESLGAFTGGIAF
                     TFLIIQKINPLGTTLILFIIACIISFTVVQQRIKRLAIVIAMVIVAFVIAHSSYVEKK
                     LLSLSFAANHPGTLLEYRRTPYQMMFVAGVDDTIALYGNNNLYAQLPDDYQVRPIIHA
                     LLSTGSVLKDTDILISGDTAFLHYVFAYAGASIDHVTVDPVMWNIVQQKINSIYPDFP
                     TKLINVYYDDTRHYLKSTGKKYNAVIILPPPPDSIMHNRLYTVEFFKDCKLHLTHNGV
                     CIIQIEGFANIMNPFLRRYVASVINSFRQVFPSSLISAGDTIYCIGFNYNRDTDTSNY
                     LSQYLKNFSASPISKTIRILVPGFSPQEFKMYFQESQQEYLAKQLQSEHIINSDLLPK
                     GYFEYLANSLKQSSSEAERIITNPLLMILLLIIIVILSLWYFNSRGTQHIIFGSIIFV
                     SGFISMAAMIIALIAYQNVFGVLYYKIALCNALFMLGLAIGSASTFTQKDFFITRIMV
                     LITGLVMLFCFTIYNNAILLYVSIILIAATTGSFIPLLLNRKTNDIQLLASTLDAADH
                     SGALCGALITPFVLLPFFGIYSLLILIVMGFILIVFRLQNYSHI"
     gene            complement(21437..22210)
                     /locus_tag="PLV81_09335"
     CDS             complement(21437..22210)
                     /locus_tag="PLV81_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10338.1"
                     /translation="MQTKQYTLTPAAGKRLIAKAIAVMPQVQEALHNKTIVIVSGTTN
                     GYVAEELLQSIGQSEGFSKQRFFRGITLPPRYKVSQSGRLEDGRTFAADVVIEKGKWI
                     KDKSLFEVVNDLQEGDIIFKGANAVNIDTKQAAVLIGHPQAGTIGVIMQAVAGRRVKL
                     FIPVGLEKRISGNINELAQLINSPQTKGIRYFPVTGTIITELEAIHILTGCHAVLFAA
                     GGVSGAEGCVWLAVTGTEEQIQKADEVIETVRYENNFVI"
     gene            complement(22322..>23470)
                     /locus_tag="PLV81_09340"
     CDS             complement(22322..>23470)
                     /locus_tag="PLV81_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOM10339.1"
                     /translation="WPDAGGEYVYLKKIFGNLPAFLTGWVSLLVGFTAPVATSSMLLV
                     QYLNTFFVNINATSVTAYTGNVFWQKTIACGIIVLFAFTHIIGVRKGSNIQNILTVIK
                     IGIVLAFIGASFYVLDSSGISRLYAHYGPLEIKTIPFVGLSLLMVMFAYTGWNGATYI
                     AGEITNPEKNLPKIMFWGVTITTILYILLNVVFLMSTDASSIMGKDEIGAIAAKALFG
                     PEASNFFSITIALILLSSVSAQMMLGPRVYFAMAKNKMIFHSLAKVNERFGTPVHAIL
                     LQMVLAIIYVYSGSAMTLVIYMGFALNVFPVLAVIGLIVARKKFPDLNRPYTTPLYPL
                     VPLTYIILSIIMMIAALCCWTITSAFAIGVLLLGIPVYYVWQRKVVNQ"
BASE COUNT         7410 a         4470 c         3829 g         7761 t
ORIGIN      
        1 aaccctaaac tcaattgaca agtcattcag tatcaatatt cttgacagca aaagtactga
       61 aatttggtgt aatggagaaa aacaactatg tcggttaaaa aagttgtatt ggcctattca
      121 ggtggcctcg atacttccgt tattgtgaaa tggttacttg aaacctataa atgcgaagta
      181 atttgttatg ctgctgatgt tggtcaggag gaagaacttg atggtcttga acaaaaggct
      241 atcaataccg gtgcttcaaa atgttatatt gaagatctta aagaagaatt tgtacgtgat
      301 tttatcttcc catgcatcaa ggcaaatgca atttatgaaa atagatactt acttggaact
      361 tccatagctc gccctgttat tgcaaaacgg caagttgaaa tagctctgaa agaaggtgct
      421 gatgctgtgt gccacggtgc aacaggcaaa ggtaatgatc aggttcgctt tgaattaacg
      481 tttaaggcac ttgcaccaca attgaagatt attgcaccat ggcgcgaatg gaatttaact
      541 tcacgatcat ctctttttga ctatgctgaa aaacacaata tacccgttcc tgtaacaaaa
      601 gataagccat acagtatgga ccgcaatatt cttcacattt catatgaggg tggaatactg
      661 gaagatccct ggcgtgaacc tgatgaaagc atgtttatat taacaaaatc accagaaaag
      721 gcacctgata taccagcata tattgaaatt gattttgaac agggtgtgcc ggttgcaata
      781 gatggcaaac gcatggatcc tgtatcatta atgaaaaccc tcaacaaaat tgctggcgaa
      841 aatggtgttg gcagaattga tatagtagaa aacaggcttg ttggcattaa atcccggggt
      901 gtatatgaaa cccccgcagg tacagtgtta catgtggcac accgcgatct tgaagctatt
      961 acgttggaca gggataccca gcatttaaaa gacaggcttg caattgaata tgcagaactt
     1021 gtatattatg gccagtggtt tacaaaacgt cgtgaagcgt tagatgcatt tattaatgaa
     1081 acccaaaaga atgtaaccgg tactgtccgc gtaaagttgt ttaaaggtac atgcatggcc
     1141 gtaggacgaa aatcaccggt taccttatat gagtcagata ttgccacatt tgatgaaagt
     1201 gcattgtatg atcacaggga tgcaactgga tttattaacc tgtttggctt gcaaacaaaa
     1261 attgaagcat tattaaagca aaaaagaaaa taatatgagc gatagtacct ttcaaaaact
     1321 ggaacagagc tatccaaacc gtatacagaa ccatgcattg tttaacgatg catggtattt
     1381 atttgtcaat gactatccac caacacactg tctacttgat tttaaagaat tgccttcaac
     1441 acgcgtagca ctgcacgcag gtgatttggg atttgcatta ctctggattg tgcaatctgt
     1501 tcttatacga tgtatcatct acaatataaa ccagctacag ccatgggaca ttcaattaca
     1561 tactactaca ataggcgcaa tggcacatac cgaaccggat actaaccgta tgattgccca
     1621 atattcagat tcaaatacac ttgtgatttc aggtaaaaag aactatattt ctggtggttc
     1681 cgatgcagat atactattgc tcactgccag acgtcctggt gaggataaat ttactaagat
     1741 gatgtatata ccggttaaaa gccttcctga aaatgcttta caggatatca atctttcaat
     1801 attgccaact atcaggcatt cttcgttaac tcttactgat tttgcctgca atgcttcgca
     1861 atgctgtcca gtaaaagaca aagaactgcg taaaatatta aagaagtgga gccttattga
     1921 acgttcactt attggtgaag catatattgc atttttatgc tatcttgcta agaatatccc
     1981 tttacctgat caggatcaaa cgcttaacga ggcagaagaa ctgttgtcgt tacaaaagaa
     2041 tttttcccgt gaacaactgg aaaatgcgtt ctcggataag tatgttgaca cattaggaga
     2101 tgtggctcgt gttctcaata ttacacagaa gattgcaaat tattgtatgg aaaatacaaa
     2161 ttctttaacc caacccatcc aattacgtat aaacgatctt aatctcttta acaaaatacg
     2221 attgtaaata cacaatttta aatcactata atattgtgtg tataactatg ccttttgtgg
     2281 agtggtgtat actcatattt ccatttttta tataattttc ttgataatta cgctatactc
     2341 atattaatgg cataaaaaag tataaagaag gcattactat gttcagctca attgaagaaa
     2401 cgatccagca attagaaaaa caaaaatata tctgttcaaa agaaattgct acaactgtat
     2461 acctggcatt gaaaatgcag aaaccagttc tggtagaagg gcctgctggt gttggcaaaa
     2521 ctgacctggg aaaatgtatt gctcaggctt taggatatga acttatacgc cttcaatgct
     2581 atgaaggcct tgatgaagca aaagcgctgt atgagtggga atatgcaaaa cagcttctct
     2641 atacccagat acttaaggat aaattatcaa aaatcttaga agatcaggct acattaaaag
     2701 atgctgtaaa caaattatca accgaagaag acgtattctt ttcagaaaaa tttcttgtag
     2761 cacgacccat cttaaaagct atcaccaatg aaacaccttc ggtattgctt attgatgaaa
     2821 ttgataaatc tgactctgaa tttgaagctt ttttgcttga ggtgttatcc gattttcagg
     2881 ttaccatccc tgaaattggt actattactg caaataacaa accttttgta ctgcttactt
     2941 caaataacgc acgtgaaatg tctgatgcac ttaaacgacg gtgtcttcat ctttacattg
     3001 actatccaac aaaggaactt gaagagcaga tagtaaaatt aaaagttcct gaatgtggta
     3061 actatctcat taaagaaata attaatacta ttcatgaaat acgaaaatta cctttgaaaa
     3121 aagatccaag tataagcgaa acattagact gggcaaaagc attagctctt cttggctgtg
     3181 aagtattaaa ccgggatatt gcaattgata cgttaaattt tattttaaaa tatgaaaagg
     3241 atattgaact ggttaagaaa aatattaata ccataatagt tcattaaacc atgatatatg
     3301 ataaagcatt cattaatgaa gaagacaaag ctaaaggtgt tttctttgaa tttgctgaag
     3361 tattaaaaaa agctggcgtt aacctgtcca ttgttgaagt aatggatatg atgcatgccc
     3421 ttcctttgat atcaattgca gaaaaagaaa ttttcaaaca aacccttgca accacattaa
     3481 ttaaggatta tactgatata cccatattca atcaatgctt tgaagagttt ttccagagaa
     3541 aaaaggggaa tgattcatta ctggaagtgt atgcttcaca tactaatact gatgatttca
     3601 ttttttccca ggaagaaaaa tccatattag aatctctcat agatgatttt ctagattcac
     3661 atccacaatc gtttttcaaa aaatctaatg aatatttaac taaaattttt attaatgaaa
     3721 tacaatcgtc agaagctggt ggtgcaatag gcttgtcgct tttcaacaga agatcgcgtt
     3781 ttgcttctgg tggcgccgaa gactcaattc aacaggaact atcgccccag gtatttgaag
     3841 ctattatggg tatgctgcga cgaagaatgt ttgaacgcaa tatgggcagt gccataaaac
     3901 aacgtgaaga ttatcttctg aacaaatata tttatcagct gaaacctgat gaaatcaaag
     3961 aaatgcgtga gcttatcaag cgctttggtc agaagatgaa aaacagaatt tcattgcgta
     4021 aaaaaaaggt aaaacggggc acgattgata ttaaaagaac attacgccgt aatcttcaat
     4081 atggaggtgt accttttaaa atttttcaca gagatcgcaa ggttgatagg ccccagcttg
     4141 ttgtcatgtg tgatatttcc agttcggtta accagtattc ccggtttatg ttgctgctca
     4201 catatacatt acagtcttta tttgcaaagg tacgcacttt tgcatttatt agcaacctgg
     4261 tggaaataac tgatttattc agggaaatgg atcccgaaag ggctttgaat tcaattttta
     4321 acgacaccaa ttttacgtat ggctggggaa gcaattatgg gaatagcttc aaccagttca
     4381 ttcagaatta cagtgatgcc ttaacaagca agacaacagt attgattctt ggagatgcac
     4441 gaaataataa tcaggatcct ggactggatt cttttatcaa aatcaaagaa cgatcacgca
     4501 atatttactg gcttaaccct gacagaaaac atttatggga ctggagtgat agtatagcca
     4561 gtttgtatat cccacattgc actgagatgc gtgaagttaa aaactttctg gacctatccc
     4621 acttcataga taccctgttt atacaaaaaa gaaattataa ataaatatgc gtatattcac
     4681 acctttacgg acactattaa tatattgttg atattaaggg tttgtataat aaatagtaca
     4741 taaacttcta tttacttgac acattaagtg ctttcaatgt aaatagatta aattaccaaa
     4801 aaatcttgat taatggtgtt gtaattatta tggcaaatac aattaaaatc ctgattattg
     4861 atgatgaaga tattatttta aaaagttgtg aaaagatttt aaaaagaaga gaagatttag
     4921 agatagatac tgcccaaaat ggctttgaag gacttgaaaa agccagatct aaaaaatatg
     4981 atatcgttat tacggactta aagatgccac agttaggtgg tatggaagta ttaaaaacat
     5041 tacgaaaaga acagccagat gttactgtca taatctttac aggatttgca acagttgata
     5101 ccgcccgaga agcactgaaa ttaggtgcct ttgattatat tcccaaacct tttacaccag
     5161 atgagttgcg gaatgttgtg gataatgcaa tcaatgcccg ccagaataag gccgaggctg
     5221 caatgcttga tttaatggca attgtttcac atgaattaaa gagcccagtt tcggtagttc
     5281 acacaacagc cgaaacactg tataaaggct attttggcaa cctatcgcct gagcagcaga
     5341 aaattattga aacaattttg cgtaactgcc agtatttaga agatattatt cgtaattatc
     5401 ttgatttgtc aaaaatggaa cttgattctt tagaatcatt tcagcaggaa attgatctgt
     5461 atgaggatgt tattaaacca attgtagaaa tgccggaaca tgcaaacaac attaaaaaca
     5521 tgaaacttaa tcttactata gaagtaaaac ctaaaataaa agctgatgca aatttgctta
     5581 aaattgttgt cacaaatttt gttaataatg caatcaaata tggcaaagca gataccacta
     5641 ttaatataga attaaaacag atacataacg aatatgtgtt ttcagttttt aatgaaggtg
     5701 ttggcattcc tcctgaaggt atggacaagc tgtttaaaaa gttttcccgt ttaaaacaaa
     5761 aaggaactga gggaataaaa ggttctggat tagggctgta tatttgtaag tcaataatcg
     5821 aaaaacataa gggaagaatc tgggctgagt cagaatacgg tagttgggta aaattttctt
     5881 tcagtttgcc tgttagctaa aaaatatgct tgaaattaaa attacaattt aatataatat
     5941 acacaaatta catacatgta aaatagtttg ggagatagct caatggaaat agaattcaag
     6001 gatattggtg aacacaaagt aatttctatc agcggtgaag tggacctgta taacgttagt
     6061 gaattgaaaa aagcattatt cagcgtaact gacggttcgt attccagtgt tgtggtagat
     6121 ttgaaagatg ttaattatat ggattcttca ggtattggtg cacttgttgc tggccagaaa
     6181 aagatgagag cacacaatgg taagtttgca ttaatgaata ttcatgatga tgtattaaat
     6241 atattaaaat tagcaactct tgataaattt tttaccatat atgaaagtga ggaggattta
     6301 ttataaattc atacgttaag ctgtacgttt taataccagt agcgtcatat catcatgcgg
     6361 aggtacttcc cctctgaaat tatccacatc ctgtacaatt aaatcagcaa gttcctttgc
     6421 attgagatgt ttatttgaaa caattaaatc ttttaatcgc ttctgcccat attcttcttt
     6481 attggcattt cgcatttctg tcactccatc agtataaagc acaacaatat ctccaacatt
     6541 taaaggtact tttgcctgtt tataatcaac atcctccatt attccaatag gcactccatc
     6601 caattttgcc agagtacagg catctaaagc agatcgatag cagaataatg gtccatgacc
     6661 tgcatttgaa aatgatagtt cgtgggtgga gcggtcataa atcatgaaaa ataatgttgc
     6721 aaatttatct acagcaaaat cagcacttaa ggaatcatta atagctttga caacatcagc
     6781 acactgaaca tcaccttttc ggatgtatga tacgaatact gtccttatca taaccataac
     6841 aagtgatgct ggaacccctt tgcctgatac gtcgcttatt aacgctccta ccctgtattg
     6901 atcaatatct atataatcaa aataatcgcc accaacacct tttgctgctc gtgtaaatcc
     6961 tttaatctct atcccctttt tattgtaaaa ccccattggg ttcagacctt cctgaatttc
     7021 tcgagctaaa tcaatctgct cctgcataat acggctttct atttccatct tctgggcaac
     7081 cttcaatcgc tcagtcattt gattaaattc tgacgccaac ttgccaattt catcagtact
     7141 atctaactct attttataat caagattacc attaccaata attactgcac cttcagtaag
     7201 ttttttaatt ggtttaataa taatacttga taacacaatt gaaccaatta cagaaagtcc
     7261 aataaacaat gcacctatta aaagaattgt aattgtcatt ttacggattt ctgataaaat
     7321 aattgcttca ctaaaaccca atcgtactgt tcctattctt actttttgta ctgtatggaa
     7381 tagaggaagg gacaaatcat aaaaattaat tcctgttttt gcatccttat atatcacgag
     7441 cattggtttt tctgtattgg tattatcaat tgcccttttg gttaaatcat catcaataat
     7501 agttccattt ttttcaggat caaaattctg aattattcgt ttattgttat ctaatacata
     7561 aatataatta acaaattcta gatttttaag atcctggaca gccccaacca gaggcaactc
     7621 atctttttga cttattgact ctctggcaat aaccgccagg cgttcagttt cacgattggc
     7681 aaactgtaat atttgttttc gaagtaattc agattcctgt tgtataaaat aatatgtcat
     7741 aacagcaata atactgcttg ccaggacaat aattgctaat gaaaacttta aacggaggct
     7801 aaaacgaact tctttagaag atgttacttt ttgttcatct ttgttgtttt tgcctttatt
     7861 ttctttactt tctttttttt cttttttaat ctttttttct tcagccatgt tttacctgcc
     7921 caataatatc aaacactttt taatgttatt ctttgctttt tcatgcgtag ggtcaattgc
     7981 taaaaccttt ctccattcag taatagccag ttcaaattga ttattgctat agtaattgat
     8041 acccttaaaa tagtattcac gaacaagttc ctgctgacgg ggtgtcaatt ttttaatttc
     8101 acgttcttca gttacttcaa agcgcaactg cgattctatt ttattaataa ttattgctac
     8161 ctgaatgtat tcaggatatt tattatttat taccctgaat tctgccagtg ctttttgcag
     8221 gttatttttt ccaccttgct ggtacaactg catagcaaca ttatatcgtc ttctaaaatc
     8281 ttcatccgtt ccttcaaaac ctgctttctt tttttcaact cgtgataatg ccattaaatt
     8341 ttgaatttca ggatactgcg ggtcaattga cgcaatcaat tcaaattttc tatatgcttc
     8401 attaaatttt ctatcagcta aatacttttt tccttcatta actatattat ctgcaaattt
     8461 cttaaatgat tcgggattct gcgctaaatc acaccgtagc aaatattcag tagcaattct
     8521 attctgtggg aatagattta atattttctg ccattgtttt cttgattcat cataattccc
     8581 ctgattatac aggcgttttc ctgattcaat caatggctga acaatggagt aatatgggtt
     8641 gttttcatct acaacttctt cttcctgcac cctcaaggcg tcaagtgcag ttgaaagata
     8701 gcgtttagcc aattcattct tagggtctaa tgttaacgta gcttgaaatg ctgctattgc
     8761 atctctatat ctaccttcag ttaatgccag taaaccttcc tggaacgttt tgtcaatttt
     8821 ttgaagtttt agtaatcgct gttgttcttc atattctttt tttaatctgg gaatcgcttc
     8881 cagctgtgct tttgcatcct tatagttgtc aattaatgcc agtgcatttt caaagttgtc
     8941 ttttgcctgg tcaaactcat atgtttttaa attatttatg ccagcaacat aaaactgttc
     9001 tgcctgtata cgtcgttgct cataggtcct tttttcttca agaatatcat ctattttgga
     9061 tatataatct ttagccactc tgtttttagc atctaaattc aatacctgca aaaattcatt
     9121 tcgtgaatcg atatattttt cctgtttaaa caattcttcc cctgatgcaa tcaacatcat
     9181 tatcttttca tattttaatc tgttgcttat ggccaattca gcctgtcgtt taccttcaag
     9241 agcaactgta tcaagcggga ctaatcgcaa tatttcatcc caaattttga tggcatcttc
     9301 gtactgcttt tccagcatta atttattagc atcttccaac agtacataat attttttctt
     9361 tatctcttcg gccattcgct ttttggccag ttccagcttt atctcattat ctaaaagttt
     9421 taaatcttca cgtaatacct gtaattcagg gtcattggga tccattctat aggcaataat
     9481 aaaattttct attgcaatct ttgaattatt tttagcattt tctacattat caggtaacac
     9541 tttccttgac aaccgataat ataactttgc tctttgcaat gatatatcct ttttatgttt
     9601 ttcctcatat acaagttcta tttttcgctg tacttcttca ttatttggat ctatttccag
     9661 tatttttaac cattcaatga tggcttttga aagttctttt ttatcataat attcctgggc
     9721 acgtttatta atctctgcga tagtgttggg attttgtgag atagcaaccg tagtcattag
     9781 ttgtgaaaaa agaataataa ataaaattac accaatcctt tgacaactgg tacacaaata
     9841 ctgcaaaact atatacgtta tgtttttacg tattttcatt atccattatt cttgatagat
     9901 aaatgtcctg tttgacaata accatcctct gttttttttg gatccggaaa tataacgaat
     9961 taattttata tcgccattaa caatttgctg ttcacggcca gtttgcctgt tatataatga
    10021 agctataaat tgtacgtttt taaattcata aggcttccca tcgttgtccg taccgtgatg
    10081 aagtttttca tccactttaa caatttttat attagcaata taatattgct gatacattgc
    10141 attgaatgta tttatatctt ttggataagc ttctaatcta ctatattcac tatagttcag
    10201 gtttctaatt gcatatgccc actgtgttaa taaattatct atatcagaat aatccttttt
    10261 cttagagcat gagataaaaa gtattgattg aataagtaac acaataaatg cttgttttaa
    10321 aattttattc attaaacgta tgactccttt gtaaataaaa tgtagttaaa aaagccatta
    10381 ttaatccact atactaatta tcgtaagttt agctcctttt cgccaaaaac tattgctgaa
    10441 aatttttcta tttttgtatt ttcccatttc catgcatctg caggcaatcc agctttgtaa
    10501 catgtgtgct ccaaaaacgt ttgtacatcc catccgtact ctgttgcaac ctgtggtaaa
    10561 agcaatcctg ccctgaatcc attacttatt ataaggccat ctctaccgat aactatctcc
    10621 cctatatcat taacaggttc aactggtgtt aataccgaaa tctctatatc tatatcgtca
    10681 agctcatctt ttcgtaaggg ctcaaaacgc ggatcctcaa atgcagatgc ttttgccata
    10741 tcaacaacag cttcaggtat ggttttaatt cccttcacat aaccaataca gccacgtaac
    10801 gcgccatatt tatgcagtgt tacaaatgca ccgcattttt gtttgaatac atcatctgca
    10861 aattcatggt tataatctac ttttggcaat gttctttttt ttaaatattc ttcaatagtc
    10921 cttcgtgcta gcgcaagtaa tttcttcttt tgttcatcat tgagctgtac catatcacct
    10981 ccttacttaa caaacgcagc agctaaatat cccacaacct catttttagg gcctgctgtg
    11041 tcgcctgaat ttgcatactg taaaatttca atcctttggg ctcccaattg tttagaagcc
    11101 accatcccag ccaacacagg gccttcaccg catgcctgtg ctttgtcgct ttgcagcatt
    11161 tcatagagat agttttcgtc aaattttttt aatgattcga taaacgtagt atcaattttt
    11221 ctggcaatat catagtgata ataatgtgaa aggtcggttg aaattataat actgtaattt
    11281 cttttctcat cctgtagaac gtgagctaac gttttaccta tctgtctgca taattctaaa
    11341 tcaatggtac cgataactat cggtactaaa acaaaatctt tcaaaactac ctgcaaaaaa
    11401 ggaagttgta cctcgagcga atgttcatat tgatgtgctt ctttaatctc aaaaaaaact
    11461 acctcatcaa gaagcctttc agcaatagta ctatccacgg taatatcacc aagaggggta
    11521 ctgaaaatac cattggtaat aattgaagca ccgttaaatc gtgcacggtg agaaggtgcc
    11581 agtacaatta caacatcagc aggattttct tttagctgtg catatgaata tgcagctacc
    11641 gggccagaat agatataccc tgcatgaggt actataaatg ctacaggatg ttccagcttt
    11701 tttacagttg cttttgataa gtaggtaatg atttcatttt ttaatttttc aggacttgcc
    11761 ggataaaaag aacctgcaaa tccgggtttg cgttgccaca tactaaaacc tccacgaatg
    11821 attatattaa atataataaa acatacccct ctttgcaagt gattttattc tggcaaatta
    11881 taaaaaaagg tgtgcataat gcacaccttt aatgtgttac tactattaat ctgatacaat
    11941 aggtaatact gcagtattga gcttagaacg ttgccttcaa ccctgctaag aaatgaaaac
    12001 ctggcattgg atattcccaa aatagtgttt gtgttggatt tgatacagca aatgtagcat
    12061 agtctttatc agtaatatta tagaaattaa caaatgctgt gtatttttcc aggattgtat
    12121 atgacagcga tgcatttgca accaggtaac tgtcaaattc ttttactccg gctgtatcag
    12181 catataatcc ttttacatat tcgccatcta ttccagcttt aaaatccttg tagcttactg
    12241 tcaggcctgc atttgcttta tgttttggta catagggtaa ataggtgtca tcctgttcat
    12301 tatcaatata cgaatatcct gcattgaacg ctaaccacgt taatgcttgc acggtaaccg
    12361 acgcttcagc tccattgtat ttaaaatctg gagcatttat ccattgagtt gtataaacaa
    12421 atttatcctt agaccatatt ctatatcctg caacatgtat attaattttc tcaataatct
    12481 gttgttccag tcctatttct gctgacgtat atgtttcagg ttttaaatct ggattggggt
    12541 ttatagcagg gaacattcct tgtaaataca cctgtcgtat atcggggaat ttaaatcctt
    12601 ttgccacatt ggtatacatt ttagtctttt ccagtggacg aactgctgca cctgcctgcc
    12661 aggctgtata atcgccatac tctgaattat aggaatatcg tgcaccaccg ttaatgctca
    12721 gaacatcctt aaaaagattt tgatccatca gggcatacac actggtatca tacatgaatt
    12781 tatcatctaa ataataagtg cctgatgacc tgtcctttgc tgtaccacca taccgccgcc
    12841 attcagcacc tgctgttaaa gtattcccct gccatagttt agcaacttcc tttaatctga
    12901 ctgcatatga agagtcactt gagtaaaatt tatcatctcc cgttgactcc tgcgttgatt
    12961 catgacgacc aaagttgcct tctgcctgta gagaaccact gtaagtacca taatcatggg
    13021 ttaatttagc cacaacgcca ccacgcgtaa catcaaactc ttctaaaagt ccaatctgtc
    13081 cagctggcat cccttctggt cccggatcat tgatatcttg cttcatgcca tagctatcaa
    13141 ctgatgcata ccagtttttg gatagagtat agccaacatg aactgttcca ttatgctcca
    13201 taaattctga tgtgtatgtt ttgccattta ttgtctgctt ccagtcgcca tcagtttttt
    13261 gaaattgata cccaacactg taatctatat taccaaactt tcccatgtgg tacaggtaat
    13321 cctcagtagt attccatgta ccataggaac cctgcaattc agttgtaaat ccatcctctt
    13381 tttgtttttt tgtgataaca ttgataacac cacccattgc acttgaacca aataataccg
    13441 gttgaggtcc aattaacact tcaattcgct caacattttt catggtcatg atatctgcaa
    13501 ccggatgtcc cataataccc atcgttgtat ccataccatt gtaaagaata ggaattccag
    13561 tagttggccc ccatcctgat ctgcctatac cacgaattga tattgtagcc gcaccacttc
    13621 gtgcaacgcc ataccccatt accctgttag acccagtata taatgatggt acctggcttt
    13681 taatgacgct tatcaggtca gtatttacac tatcttttac tgtactacct gaaattgaat
    13741 aatacgcacc tggatacaat acagtatcag acacaattac atcatctaac tgaataacat
    13801 tttccccttc ctgagcaaat acagggatat acaatagtaa aattgctaca aaacataaga
    13861 ctaaacgttt cataagcacc atccttaaaa attatttaat ttatagtgct acatttatta
    13921 taatagtaat atttgtcaat acaatatttt aattttttat ttgtatagtt tctttatttt
    13981 ttattcaggt ttatttcgat ttgttatccg ctgtttagct attgataatc ctttttctat
    14041 ctcactccga ctttgctcac cttcaagtct gtacaattcc atgccattgc catctaaaaa
    14101 aagatatact ggtatcctcc ataaatgata ctgtgataca tacattgcac cttcgggatt
    14161 gtgtatatcc acttttttta tttcaagtgc tctgtctata cgctgaagat attcaatagt
    14221 atcatcaatt gcagagcatg aatcacaatt ataggcactc acaactacaa gcttaattgt
    14281 ttgttgagta tcttttttag cagaacatcc aatactatga actatcgcac atataagtaa
    14341 aactatataa ataatatttt tattcatagt tatcagttac tgaacaccaa tgtcgcccat
    14401 gctttctttt tcaagaaact caatgcgctt tcgtttttgc tcatcatccc attctttatc
    14461 agataaacct tgtaaactgc ttaccttagt tttatattca ccatcatacg gtttcatcat
    14521 ttccctgtcc ataaattccg gatctggcgt cgttgcttca atgaatctgt agccacgtaa
    14581 atcccgcaac acaaaaggtg gttttgcttt tttatctaaa agtactttcc caaaaaataa
    14641 aagaggaagc cactgcctgc cagcttgtaa ttcccgtttt tctattgtat aagctacagg
    14701 ttcaagttca ttatcaaaaa gatttgcatc aataatataa tagccttttc ttaatacatc
    14761 aatttcaaca tcaatataga aagaaccatt ttttatgtag tccctgaaat ttccggtaaa
    14821 ctttgccggt atggtatttt ccgggaaata ttcaaatggt atagcggcat caattacttc
    14881 atctgcagcc ttaaattcaa cctgtgccca gtatgtacct gatttcttat ctgatgtaaa
    14941 tacctgtgca cagttaacat cagcagtata gtcaccaaca ttatttttta tgagcgatag
    15001 ctccgacaca tattttgcag tttttatatc aatacctttt ttgataattg atttagtaat
    15061 tgttactggc actctatccg agtcgggctt tgtaccctga agtaccataa gcacaaatgt
    15121 gattggttca tcaccaatga ctcgatactt gtccgcagta aaaagataat agatgtcact
    15181 gtctttttct attggtaatg gttgctcata acgacgattt ggcataagta aatcgatgtt
    15241 ttctttactc agaggcctgc tgtttggggg ataaattgct aattgcttat aatcctgaag
    15301 tgcctgttgt ggtgaaatga gtcccatctg ttccagcact gttccatagc cttttaaact
    15361 atcgcctgcc gaaagtgttg aaggttggtt attattttct gattttgaaa tcagcataat
    15421 aatcactata attataataa ccaacagtat tgctatatga ctccctttta ttttcatagt
    15481 atcctcttac cattactaca tagttctaaa tatcatactt tccattaaaa accattaaaa
    15541 accattttat tctaaagtat tctttagtat cctttaattt ctttaattcg gtataaagaa
    15601 cattgctatt tgaaattatg aaaaaaagcg cggctctttc aaaccgcgcc cgtggcagga
    15661 aaaataatgt caactaccat cctaactgat atataaattt cattttcatt ccatagtgat
    15721 ccagataata accagtacat gtataggcaa taacatgatt tgtccagtga gtacatgtac
    15781 ataaactatt atagcttcct gaatttacgc atgctcctga tgaatgataa gctactgctc
    15841 catcatcctc cccaggtaac aatacagatg caccaaaaat tcccttatat ccacctatgt
    15901 gataaattgt tataccattg gtatcattat ggttatacat attacgaact gtactaacac
    15961 ccaactgccc tgctaagtta caggtgaaaa gatcggatat gccttttatt gctatttcac
    16021 ttccgccacc agcaccagcg gttgtatata catacgcaat attccactgt ggagtataat
    16081 tggcattgat ataccccatg ataacatcac ccgcactgta gttaacgata taacaccagt
    16141 tgcttccctt gcaatataca tccagatagt tggttttaaa ctgactggct gaatatgcta
    16201 tggtattgcg attgtccaca taacagttga tagaagtata tcctgattta ctagcaagac
    16261 gcgaagtacc tttgccatca agccagttag ttgagcacat gccatttaaa tgcaaaatat
    16321 aggttgtgct gaaaccggct gaagcaataa acacaaaacc tgccacgaaa agcaatacta
    16381 cagcaaacct cttcataaac cccccatatc tataatataa ttataccata tagacggtat
    16441 gtataaatca tcgaaatatt atgtcaattt tgtcaagatt tttttttaaa tatttttcga
    16501 taacgattta tatattttat gaatttatac ttgattctta tactattatt tcatatttca
    16561 atttgacaat taaaaaataa tattttattt tatcaacatg aatggtatac cagtaatagc
    16621 agtacttata ttactttttt ctttacaatg cactgaaagc tatagcatta ccaaacaatc
    16681 taaattagca ggatcatggt atccttccga taaaaatgaa cttttgaaat acgtttcatc
    16741 atcagaacat ccatcacagc aaatatcaca cattccactc ataatagttc ctcatgcagg
    16801 ctatatctat agtgctcatg ttgctgcaaa agcttacagt gctatagcaa gctataaacc
    16861 tgatattatt gtcataatag gaccatctca ctacgaatat tttactggat ttgcccttcc
    16921 cgtttatgat gctatcaaaa ctcctttagg tgttgttact gttgaacaaa aagtaataca
    16981 cacattgtca caaaacaata cttttacgtt taacaacaat gcctttgatc ccgaacattc
    17041 tatcgaaatt caattgccgt ttattcagca ttatttctct gggatcccca tccttcctgt
    17101 gcttgcaggc aatgtaacaa tacagcaagc cagacaggca gctatgcaat tattaccggc
    17161 aatagcgcac tataaaaagc cactatttgt cataagcagc gattttaccc accatggcca
    17221 acgatttagc tatgctccct attacaagct gcaaccagat aagcgtatgg aagatatcaa
    17281 acgtgatgat atgaaagcaa ttgattatat actttcaaaa aacactgaaa aattttataa
    17341 ttactgccag tcaacaggca ttacaatatg tggtcataat gcaatagtgt tagggctgtt
    17401 attattgtca aaggtatata caaaagaatt agtagcgtat tcaacgtcag ccagcattac
    17461 taacgactac gataacacgg taagctatgc agctgtagta ataacaggtg aaattaatac
    17521 cataagcaca agcacattgg gcgatagttc ctgtaatata tccagtgaag ataaaaaatt
    17581 tttgctttca ttagctcgtc aatccattga aaccatgctg aagaataaaa cattagtaga
    17641 atataaagga acaatccctg aacactgtaa acaaaacgca ggtgcctttg ttacattaac
    17701 tattaatggt aatcttaggg gttgtattgg ttatattgat ccagtaaaac ctctttatca
    17761 aaccgttatt gagtgtgctg cacatgctgc ttttgaagat ccacgcttta aaccacttac
    17821 aaaagatgaa tataaaaaaa tacatattga aatatctgtg ttatcgccat ataaaccaat
    17881 tgcatcactg gatgaattgc gtctgggaac tgatggcctt attgttgtaa aaggcaacag
    17941 gcggggtgtt ttacttccac aggtagcaac agaatattgt ttaacaaaac agcaattttt
    18001 tcagcacacg tgtgaaaagg caggaattta tccatttgaa tatgactctg taacaatgta
    18061 cacgtttact gctgcaattt tcagtgaaaa tgaaatcatg aaataacatt ttatgatgtt
    18121 tttaatgtaa cattggttct atccaataag aatctggatt gtaaatatga gatctgttac
    18181 tcgtaaacaa tttttacata gctgtttata ttcaatgcaa gcgcttgtgg ttggctcagt
    18241 tattccattc ctgccagaag atgttattgc cagtaactat cgccaggctt tacactggaa
    18301 aaaaattgat gcacaaaccg tgcagtgtaa cctgtgtccc aatcaatgca tgctaacaaa
    18361 cggcacaagg ggtttatgca aagcccgaca gtgcatggat ggcaaactat acagcctggt
    18421 atacggacgt attgctgcat atcatataga cccaattgaa aaaaagccat tgtatcattt
    18481 tttgccaggc aatgatgcat tgtcagttgc aaccgctggc tgtaatttta gctgcaaatt
    18541 ttgccagaac tggcagcttt cacagtcaca gcctgaaaaa cttgacagcc agtatataac
    18601 ccctgttcag cttgtaaagc aggctaaaga acacaaaacg ccagtcattg cctttacata
    18661 caatgaacca tcaattcagt atgagtacat ctacgacact gtacagatag caaaaaaaga
    18721 caacatatcc tgtgtcatca tttctaatgg ttatctcaat aaacatgcca gcgagatgtt
    18781 atatccgttg ctcaatgcca tcaagattga tttcaaagca tattcacaga aattttattc
    18841 tgaaatatgt ggtgggaatc tatcgccagt actttcaaat attgaactgg ctaagaaaaa
    18901 aaatgtatgg cttgaactgg taaatctagt tattcccaca ttaaatgaca atcctgatga
    18961 tatcggggca atggttcgtt ttattaaaac tcattgtggc agaacaacgc ccctgcactt
    19021 cacacggttt tatccaatat acaaattaaa aaatattccg cctaccccag tatcaactct
    19081 tgaacgctgc agggaaattg ctcttgctga aggacttaaa tatgtgtata taggcaacgt
    19141 cccagggcat agcgccaaca atacctattg ccataactgc ggcaaacctg ttatagaacg
    19201 aagcggtttt tatacaacaa aaaacaaact taaatcaggg aaatgcccat actgcaatac
    19261 tgcaatccct ggtatatgga cattatgaaa tacaaccgtg tatttttata tatatctacg
    19321 ttcttcattg gcatttcagg tagcattgcc caggcaatta tgattcgtga attactcatg
    19381 tttttcaggg gcaatgaact atctattggt attattatgg tattgtggtt tgctggtatc
    19441 accgctggtg cagccataag caaaaaaata aaagcgcatc aattcagcag tattgcccat
    19501 aacattttac ttcatcccag ctcagtatgt atttcgctta tactgctata ctgtacacct
    19561 atattttatc acacatatga ttcattttat ccacttgaag tggaacttat tattgcagtg
    19621 gtgtgtcttt taccgcaatg ctttattatt ggatatatct ttccagtttt agttaatgca
    19681 tgctcacgtt taacaattaa caatcccgga actgtagtat ttttacttga atcacttggc
    19741 gcatttactg gtggaatagc atttacattt ttaatcatac aaaaaattaa ccctttggga
    19801 accacactca ttcttttcat cattgcctgc attatttcat tcactgttgt acaacaaaga
    19861 atcaagcgtt tggcgatagt tattgctatg gttattgttg cctttgtaat agctcactca
    19921 tcatacgttg agaagaaact gctctcactg tcatttgctg caaatcatcc tggtacacta
    19981 cttgaatata gacgtacccc ctatcagatg atgtttgttg caggtgttga tgacaccatt
    20041 gcactgtatg gaaacaataa cctgtatgca caattaccag acgactacca ggtgcgaccg
    20101 ataattcatg cattactttc cacgggctct gtgttaaaag atacagatat cctcatttca
    20161 ggagatactg ccttcctgca ttacgttttt gcatatgcag gtgcttctat tgaccatgtt
    20221 actgttgacc cggtgatgtg gaatattgta cagcaaaaaa taaattcaat atatccagat
    20281 ttccccacaa aattaataaa tgtttactat gacgatacac gccattactt aaaatcaaca
    20341 ggcaaaaaat ataatgctgt catcattttg ccaccacccc ctgacagcat tatgcataac
    20401 cggttatata cagtagaatt ttttaaagac tgtaaactac atttaacaca taatggtgtg
    20461 tgtattatcc agattgaagg ctttgccaat attatgaatc cttttcttag gagatatgtt
    20521 gcatcagtta taaatagctt caggcaagta ttcccttcat cacttatttc agcaggggat
    20581 actatttact gtattggatt taattacaac cgtgatactg ataccagtaa ctatctgtca
    20641 caatatttaa agaatttttc agcatctcct atttcaaaaa caatacgcat tcttgttccg
    20701 ggctttagtc cccaggaatt taaaatgtat tttcaggaat cgcaacagga atatcttgca
    20761 aaacaattac aatctgaaca tatcataaac agtgaccttt taccaaaagg atactttgaa
    20821 tatttagcta attcattaaa acaatccagt tcagaagcag aacgtattat taccaatcct
    20881 ttgttgatga tacttttgtt aataataata gtaattttaa gtttatggta tttcaattca
    20941 cggggaactc aacacataat ttttggcagc ataatatttg taagcggatt tatcagcatg
    21001 gcagcaatga tcattgcact tattgcgtat caaaatgtgt ttggtgtttt gtattataaa
    21061 attgctcttt gtaatgcatt gtttatgtta ggattagcaa taggcagtgc atccacattt
    21121 acacaaaaag atttttttat tacacggatt atggtattaa ttactggctt agtgatgtta
    21181 ttttgtttta ctatctacaa taacgcaata ctattgtatg tttcaattat actcattgca
    21241 gcaaccaccg gttcttttat accactatta cttaacagga aaaccaatga tatacagctt
    21301 ttagcttcaa ctcttgatgc tgccgatcac agtggtgcat tatgtggtgc attgataaca
    21361 ccatttgtat tgcttccgtt ttttgggatt tattcgctat tgatattgat tgtaatgggg
    21421 tttattctga ttgtttttag attacaaaat tattctcata tctgactgtc tctataactt
    21481 catcagcttt ttgtatttgc tcttctgtac ctgtaacagc taaccataca caaccttcag
    21541 caccacttac tccccctgct gcaaaaagca cagcatgaca tcccgtaagt atatgaatag
    21601 cctcaagttc tgttatgata gtgcctgtta ccgggaaata gcgtattccc ttggtttgcg
    21661 gtgaattgat aagctgtgca agctcattta tgtttcctga tattcttttt tccaatccaa
    21721 cagggataaa taatttgact ctgcgaccag caactgcctg catgataaca ccaatagtgc
    21781 ctgcttgggg atgacctata agcactgcag cctgtttggt atcaatgttt actgcatttg
    21841 ctcctttgaa aataatatca ccttcctgca aatcatttac tacttcaaat aatgatttat
    21901 cttttatcca ttttcccttt tctattacaa catcagcggc aaatgttcta ccatcttcca
    21961 gtctcccaga ttgacttacc ttatatcgtg gcggaagtgt tatacctcta aaaaaacgct
    22021 gcttagaaaa accttcactc tggcctatgc tttgtaaaag ctcttcagca acatagccgt
    22081 ttgtagtacc tgaaacgata acgatggttt tgttgtgcaa tgcttcctgc acctgtggca
    22141 taacagcaat agctttagca ataaggcgct tgcctgctgc aggggtaaga gtgtattgtt
    22201 tggtttgcat agtcgtaatc tatatattat ttacaattat ttacaatagt ttacaataag
    22261 tttattatta ctaaattaaa acagatgtgt tacattttca aagtaaaaaa atatataagc
    22321 tctattgatt gacaactttt ctttgccata cataataaac aggtattcct aaaagcaaca
    22381 ctccaattgc aaatgcagag gtaattgtcc agcagcacaa tgcagcaatc atcataatga
    22441 ttgaaagaat aatgtacgtt aaaggtacta acggatacag tggtgtggta tatggtctgt
    22501 tcaaatcagg aaattttttg cgtgctacaa taagtcctat aactgccagt acggggaata
    22561 cattaagcgc aaatcccata tagattacca gtgtcattgc actaccggaa tatacataga
    22621 tgattgccag caccatttgt aatagtattg catgtaccgg cgttccaaaa cgttcattaa
    22681 cttttgccag tgaatgaaat atcattttat tttttgccat tgcaaagtaa acacgcggtc
    22741 ccaacatcat ctgtgctgaa actgatgata gtaatatcag agcgatagtt atgctgaaaa
    22801 agttacttgc ttcaggacca aaaagggctt ttgcagctat tgcacctatt tcatctttgc
    22861 ccataataga tgatgcatct gtggacatca aaaagactac attcagcagg atgtataata
    22921 ttgtagtaat agttactccc cagaacatta tctttggaag atttttttct ggattggtga
    22981 tttctcctgc aatataggtt gcaccattcc atcctgtgta tgcaaacatc accataagca
    23041 gcgataatcc aacaaatggt atggttttta tttccagtgg tccatagtgt gcatataggc
    23101 gactgatgcc ggaactatcg agcacataaa aacttgcacc aatgaatgca aggacaatgc
    23161 caatttttat cactgtaagt atgttctgga tattgctacc ctttctgact ccaatgatat
    23221 gggtaaacgc aaacagcaca ataattccac aggcgatagt tttttgccag aaaacattgc
    23281 cagtgtatgc agtgacggaa gttgcattga tattgacaaa aaatgtattg agatactgga
    23341 ccagtagcat ggaacttgta gcaacgggtg cagtaaatcc aactaacagt gaaacccacc
    23401 ctgttaaaaa tgctggaagg ttaccaaata tttttttcag gtacacatac tcaccaccag
    23461 catcaggcca
//
LOCUS       DAORYL010000046        23392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1835_length_23392_cov_12.945837, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000046 DAORYL010000000
VERSION     DAORYL010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 23392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23392
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1835_length_23392_cov_12.945837"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PLV81_09345"
     CDS             complement(<1..533)
                     /locus_tag="PLV81_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10340.1"
                     /translation="MKVKLKILKSDIERQLKEKYYLKKKQWSKKWDKFLEHGHEKMTI
                     MFIPHNEQKIFNFQISRFTILFFSSLMMIVIVTSAYAIIKNAAVKREEERLLSNYKDI
                     RSQLIRFEQLTDELDKELDDIKPDIEDLYELATNNNNTRSIWESSMISVEDPSMRQYS
                     AILPSEVKTLRVLQKDL"
     gene            705..1073
                     /locus_tag="PLV81_09350"
     CDS             705..1073
                     /locus_tag="PLV81_09350"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOM10341.1"
                     /translation="MEFGKHMQCKRCGTCCNDVRLAESPEMLKKAYEYWLRMPSVDPK
                     FSEIYLIYPMLTFIYENQSEDLPYHYSCKHFTRDSNGLGVCSIYEIRPRMCRDFPYYE
                     GVELAPEDNVSPYQGCGYNE"
     gene            1156..1701
                     /locus_tag="PLV81_09355"
     CDS             1156..1701
                     /locus_tag="PLV81_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HOM10342.1"
                     /translation="MIAEDKKNKLLAIVFYSMIAAYSYLVLTIGSGNDILFWSYEEFI
                     FGTVISIILASVTYWLIPIKITGRLLNPFFYLGLIVYFAGPFFIALLIANVEVMYRII
                     SGKIKPAIVKVDPEIQDEMGVYLLVNSITLSPGTLTIGTDPEKRKLFIHCLYWNKSKD
                     YAAVPKDVAGFLHYFLKKLFR"
     gene            1715..1984
                     /locus_tag="PLV81_09360"
     CDS             1715..1984
                     /locus_tag="PLV81_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013467770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HOM10343.1"
                     /translation="MYDFNSIFLLSALLCMVYVCIIVIRIAFSHNVPERIVALDTINT
                     LIIVIMIVLGAAQKKALYIDIGIVYGIISFIGTLYIARYLIDERK"
     gene            1985..2392
                     /gene="mnhG"
                     /locus_tag="PLV81_09365"
     CDS             1985..2392
                     /gene="mnhG"
                     /locus_tag="PLV81_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012572546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HOM10344.1"
                     /translation="MDTVIISCLVIGLVVNGMSIIGLMRFPDIYTRLHAATKTTTFGS
                     IFVVLAIVIKNIIYYDQASLTIAIHSIVALLVIIFTNPISAHAIARASLLSGIKPFGV
                     VINEFDYKSQIKTADELELEKVSEGIPDEDGVL"
     gene            2389..2631
                     /locus_tag="PLV81_09370"
     CDS             2389..2631
                     /locus_tag="PLV81_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HOM10345.1"
                     /translation="MMYYFFLVISIALVITALLSIYFRDLLAAIVSYSVFSLVLTVLY
                     FLLDAPDVAIAEAGIGAALTTCIFVIAIRMTRRREE"
     gene            2628..3461
                     /locus_tag="PLV81_09375"
     CDS             2628..3461
                     /locus_tag="PLV81_09375"
                     /inference="COORDINATES: protein motif:HMM:NF006248.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HOM10346.1"
                     /translation="MRLLSVVAIVILFGFLFVGILHLHPVGEALGNSEVLQGRPELGI
                     DINGVSTMDDYFLRNGQIETKSNNIVASVVFDYRGFDTLGEATVLFIVVSSISMLFFT
                     FLKSKSIVPAGVPPAGQFPKIESRVITFGSFIMYIMILSFGVYLVVHGHISPGGGFQG
                     GSVMASATALLLISFLITRHMQRTRKIFSFFESLGLTIFIGLGFAGITLSFLYNFLAH
                     TSTGFGRIIAFGPNQGYLISAGILPLLSLAVGIEVFFGLSLIVVTLFHVSGVKDTNSL
                     R"
     gene            3467..3826
                     /locus_tag="PLV81_09380"
     CDS             3467..3826
                     /locus_tag="PLV81_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HOM10347.1"
                     /translation="MSAIVLYVTFILLLTIGIIALLFKRNLIKIVLAFNILDSGINLF
                     FIALGYRENAMAPIFTLAPLESVITMVLPTPQALILTNIVIGFATTALMLGVSVLTYR
                     NNNTLDTRKLRGVEEYD"
     gene            3819..5345
                     /locus_tag="PLV81_09385"
     CDS             3819..5345
                     /locus_tag="PLV81_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOM10348.1"
                     /translation="MIKHVPVLLVAIPLLAAFMIPMIGMVNRTARTVVGAIALVMILA
                     ASYYIAGDILVHGRILYYMVGSSNPEMLTEHGLTFPIRIGLKIDPFSLFFIIMTTTLV
                     LIFYVFSAQYIEENERKNYFTVLFITMVAGMMGLLVANDLFTFFVFLEILSISSAALV
                     AFRTERKHPPYAGYKYMFVSAVATSFFLLGVAFLYAQYGSFNIDYLKEAIRGTLLDKI
                     AIVLLIIPLAMKSGVVPMHMWIPDTYSEAPAPISAMVKLGSLICLYGLFRISFSFGMS
                     HAHVSYPLGVLFIVFGILSMFVGVTQALVQKDVKRLMAFHAVSQVGYMLLGMGVGLTV
                     VKSGDASFALFGKVAMIGGLLHVVNNALYKGLLFLTSGIMVRHFKTRNLDAMHGLAHY
                     DVFTTVVFMIAALAISGIPPFNGFVSKIIIYESVFQYNPVLTLIAIFVSILTLASFMK
                     VFYSAFLGMPSSRRPMEQPVLIKCSMFVLAVFIVVIGIYPQGILKAIIEPAVNALINF
                     "
     gene            5356..5694
                     /locus_tag="PLV81_09390"
     CDS             5356..5694
                     /locus_tag="PLV81_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10349.1"
                     /translation="MNILQVEYLHWAPLFWLGIFIGLAVVVVIIGKLFFRNDYNTVDQ
                     QSLPFYSGTSNPLLQLITASSLYWGFKHMLDAYFLIIKRLFISDVEDFIQWFIIIVAG
                     MLLILTGVLV"
     gene            5742..6122
                     /locus_tag="PLV81_09395"
     CDS             5742..6122
                     /locus_tag="PLV81_09395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOM10350.1"
                     /translation="MNTGSCNACDIEILATLMPRYDAERFGIKLVGSPRHADVLLVTG
                     TVTEKIKDNILQVYEQVPEPKLVIVIGGCGSTKGVFQESYAMAGPVDAILPVDVYIPG
                     CPPRPEAIIYGIAKAWEKLEKLQK"
     gene            6141..6635
                     /locus_tag="PLV81_09400"
     CDS             6141..6635
                     /locus_tag="PLV81_09400"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOM10351.1"
                     /translation="MKTESIVTVLQNKLGSHIQASREKITNYGNGINRVTLWISIDRE
                     SFHSSIAILKTMGRLHISTPMPYKEYEDRIELIYPFALKEKDTKFSEIMVIISVDIPK
                     DDLKIRTITDIVPGALFMERETMEMIGVEIEDIPDPRRLFTGNYLPDGVYPLRTPKNK
                     ESMQ"
     gene            6632..7870
                     /locus_tag="PLV81_09405"
     CDS             6632..7870
                     /locus_tag="PLV81_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOM10352.1"
                     /translation="MKSDITYPLNIGPVHPAFKEPIKFTFRIEGEKVVGADIDFGYTH
                     RAIERIAQERNYIQIIYLLERVCGICSFSHPMAYCLAIEDVAGIEVPPRARYIRTIVG
                     ELERLHSHLLWTGVAAHEIGFDTLFMYTWNIREKVLDCLEELTGNRINYAMLTIGGVR
                     RDITADNAQTIHQVLEYYEELYNRTSEILLDDLTLRARIEGVGILTAEQAERLCAVGP
                     TARGSGLMKDVRVDYPVNAYYEIPWLKPVSPRDIGKEPIGDVYDRMAVRVLEIQQAIK
                     IIQFCMENLPEGDINTGSGAVKMINALKKLEGEGVGRYEAPRGEVCHYVILDNQEHPV
                     QIKVKAPTYSNGFTWAPMLTNIEIADIPIVVASIDPCVACADRMTYVQADGSHTTISW
                     EELRAKSIQKYKGVRRQWMK"
     gene            7858..8871
                     /locus_tag="PLV81_09410"
     CDS             7858..8871
                     /locus_tag="PLV81_09410"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOM10353.1"
                     /translation="MDEVIGVAIAIVVAGVFAGLFLKGIDRKLAAFMQSRIGPPIAQP
                     IYDILKLMQKQTIIPSTSVRWLFTGAPLVALTSGIVLLLYISLSYVCYIIGLQSPVQG
                     DLIVIIYLILIPSIAIISGAFASGSPYASIGAQREMVILMSTELPIAVVVLSIAWHVA
                     DSMYNPFALQSLFEYTLWHHAGAFGILGGILLFLAMILVLPAEMSKVPFDQAEAETEI
                     AEGVMVEYSGKLLAFLLLTDAFKALSLSALIVILFFPYTFTGLFGLSFSIGSYSITPL
                     IEIIFFIGKVVIVYSIGVTVIRVIMARLKISQVAKVFLFAVSAISIIGMVLIMLDPKM
                     KMI"
     gene            8882..9349
                     /locus_tag="PLV81_09415"
     CDS             8882..9349
                     /locus_tag="PLV81_09415"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOM10354.1"
                     /translation="MLGMVYKVVEQLFAKKSTNYFPSKYILEDTADTLKKGTIHPPVA
                     VKEGFRGRLEYNVESCTGCGLCSKVCPANAIELYPAVINDKKTKRIVIYLSRCTYCQE
                     CVNICPKNSIQITTHFFMADYNKYDASLVVGLEHRKKYEVKEEGSPDVQKEGN"
     gene            9327..9590
                     /locus_tag="PLV81_09420"
     CDS             9327..9590
                     /locus_tag="PLV81_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10355.1"
                     /translation="MFKKKEIEIKVKLYSGLHKDASIDRYNPDEGYILKIPKGSRVRY
                     VVKQLGLPDIYSLAYFCNGQRIGIFTKLREGDELSCFRPSGGG"
     gene            complement(9592..10317)
                     /locus_tag="PLV81_09425"
     CDS             complement(9592..10317)
                     /locus_tag="PLV81_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HOM10356.1"
                     /translation="MSTLTNKHITKLLKVVGKAINTYSMISSNDNVLIALSGGKDSLV
                     LMDALHRRLQHIPITYNLYCCHVSVTNFHNKETIETLRTYCQHYSIPLYVKEITLDLS
                     DTNSSPCFVCSWFRRKALFETAAQLQCKKIAFGHNQDDIIVTFFMNMLYHGKLSTMPP
                     KLKMFNGALEIIRPLAFLTEAEVKGYCNALPLVPLENDCPYKDTTRRKQTKELLKIIN
                     KSVKGAQSSIFRSLRHRDDEYLL"
     gene            10426..10782
                     /locus_tag="PLV81_09430"
     CDS             10426..10782
                     /locus_tag="PLV81_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM10357.1"
                     /translation="MARCMIVDDSSFMRRIIRQTLQEGGHEVVAEVDNGLEALEKYRE
                     IQPDVVTMDITMWGKDGLEAISEITDSYPDAKIIVISALSERTLKINEKDIKARVFLT
                     KPFEKKELLEAINKVL"
     gene            10784..11830
                     /locus_tag="PLV81_09435"
     CDS             10784..11830
                     /locus_tag="PLV81_09435"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOM10358.1"
                     /translation="MEEQLLQLYKKSTVADSAYLIADGKAYLFVTEADKYLLQGKNLI
                     IGATELVLGAKTNENILRLETALALKGTTIKKIPAATLIQGLDNSAFAMNLAIVEAKM
                     LVLTNEILKKDLQLDSQLVKEKNICIEYFTIIQSLQKEYDKRRLPWIKELIEKYKTSL
                     IYKKGEAFTRATESLKVETPRELSSNTIEFPPGSVLCQEGSIGDFMYILESGQLEVYI
                     QNAKVATIAEKGTVIGEIAMLLSMPRTATLKAQNTVLVTKVTRDDLKQSSSPQLIQML
                     LVSLAKKHQANVMHIEDINEKKALAYAEKKEQEPHKTDIHRYINELSQLKSGLEKLIN
                     TKQADYLEYILKIK"
     gene            complement(11834..12367)
                     /locus_tag="PLV81_09440"
     CDS             complement(11834..12367)
                     /locus_tag="PLV81_09440"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOM10359.1"
                     /translation="MKIEITILGLLMEENLYGYEIKKRIVERLEDYVDIKFGSIYYAI
                     KKALDNGWVKKIGTEKEGGNPERYIYQILPDGRKYYKKMLKQYFDHNFIHFDIDIVLM
                     FLNSLSPEQREQFIEDRLEHIKEKMNALREKIEAESKNTSPASQLHVYTYLENHLKAE
                     LNWLRSLKKGEVLTEVE"
     gene            complement(12563..13150)
                     /gene="ruvA"
                     /locus_tag="PLV81_09445"
     CDS             complement(12563..13150)
                     /gene="ruvA"
                     /locus_tag="PLV81_09445"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOM10360.1"
                     /translation="MISFLKGNILSCKPTRVVLDVQGVGYEIHIPLSTYEKISTARST
                     ELFIHMVVRENAILLYGFNSEEEQQIFSLLLTISGIGPSIAISILSSMTPQQILSAVK
                     NDQITAFTSIPGIGQSKSEKIIFELKRKLKKLDTILSRESTTTPYMKDAIEALVSLGF
                     DEKQASSTVSSVIAQHSDINNIETIIRLSLKQLSK"
     gene            complement(13178..13663)
                     /gene="ruvC"
                     /locus_tag="PLV81_09450"
     CDS             complement(13178..13663)
                     /gene="ruvC"
                     /locus_tag="PLV81_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008537374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOM10361.1"
                     /translation="MAYLLGIDPGYGRCGWAIISDSFSYIDCGVIETKPTDENRLLDV
                     YTSLSDIIQKYSPVSCGVEKLFFNKNVKTAIPVAQVIGVIKLLLAQHNIPYAEYTPVQ
                     IKQSITGYGRSSKDQLAFMVKKILKTPEIIQYDDAFDALAIALCHCLALPGYSIIKKN
                     I"
     gene            complement(13670..14416)
                     /locus_tag="PLV81_09455"
     CDS             complement(13670..14416)
                     /locus_tag="PLV81_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOM10362.1"
                     /translation="MSGHSKWANIKHRKAAQDAKKGKVFTKLIREITVAARVGGDDLN
                     SNPRLRTAVLKARENNMPMDNIERAIKKGTGALEGSTYEEVRYEGYGPGGVAIMVECM
                     TDNKNRTTPEIRTIFSKNGGNLGETGCVAYLFDKKGVINIDANATTEDNVMEILLDYD
                     IEDIKTEDNTIVVYTAPEKFNDILEVIRSNNLPTLNSEITYVPQTTVQLEGQKASQCL
                     RLIEALEDHDDVQSVHANFDIPDDIINNMS"
     gene            complement(14538..15818)
                     /locus_tag="PLV81_09460"
     CDS             complement(14538..15818)
                     /locus_tag="PLV81_09460"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10363.1"
                     /translation="MKFKSLILYLSIFSILLLIDNGSGQDYVALIQQNKFTDALQIIQ
                     LQLDEMYSSRSIDKKVPDSYVAIGKIEEGIDLKKLFTDRKLQPYFIENNDTLYTLHIN
                     AALCYLNMFKYKEAVQHYFQALRFTTISEKDHTIFYSLALLFKRLNKYDAYLTYLEEA
                     YEIKPDNYDYSLELALALASGKNKKKALFHLNRYIQAKGNDTPPELYLTAANCYESIG
                     DFINAGRYYQLYLTIHPDDAAIQFALGYLAYTKISDMKLAYTSLSKGLSLYGDSDLIR
                     KGISHAIIGDITSMDLNYTESLNHYLQAIQISERIHQSIEDKKNSIEGIKIKINTIKS
                     TLLDKKDISLYPEYQSLMDELGNNELELRRLEHEYSKLTVGELYFKIASTYENTMQYQ
                     QAIDWYTRAIASGTKIRESSKKIEKIRLKISRGY"
     gene            complement(15884..16498)
                     /gene="pth"
                     /locus_tag="PLV81_09465"
     CDS             complement(15884..16498)
                     /gene="pth"
                     /locus_tag="PLV81_09465"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOM10364.1"
                     /translation="MKLIVGFGNPGEEYFNNRQNIGFKVVDILGNNENIDIRIKKKKS
                     IIGRGKISGEDVVLLKPQTFVTLLGESVLYIASFLRINVRDIICVLEDPSLPLGEIRI
                     DSIMSKLKHPGIESITRALKSDRFAKVRIGIGYPKKGVTMEEHLLNDFSEDENFILID
                     VLNKAEEVVRMLITHSIEDVQNKYNPEGAPKIKKRQLPKIRIRR"
     gene            complement(16523..17146)
                     /locus_tag="PLV81_09470"
     CDS             complement(16523..17146)
                     /locus_tag="PLV81_09470"
                     /inference="COORDINATES: protein motif:HMM:NF004139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOM10365.1"
                     /translation="MEAHVLPVETRTQIGKNANHKLRAQGYIPAVLYSHGESQTIMVQ
                     KKNFFKIFKGHISENVLIDLDIKDSKGSPVKAFVKDYQRHPITDEILHVDFFRVTMTE
                     AISTKVPVEISGTSIGVKQGGILEIIEREIEVECLPADLPEKITIDVTNLSIGQSIHV
                     KDIAAPKGVTILSTPETVIVAVLAPHKAAEEVAQPVAEEVQPAEEQQ"
     gene            complement(17220..18167)
                     /locus_tag="PLV81_09475"
     CDS             complement(17220..18167)
                     /locus_tag="PLV81_09475"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOM10366.1"
                     /translation="MIYPIKLFSGTSNFPLAQEIAQYLGIELSEIEIKKFKDGEISVK
                     ILENVRSVDVFLIQSTSNPANDNIMELLLMVDAAMRASAQRITAVIPYYGYARQDRKV
                     EPRVPISAKLVANMLQAAGVDRILTMELHSDQIQGFFDIPVDNLFASPLVIEYVRELN
                     ILNPTVVSPDAGGTERARFLARNIGAELAIVDKRRPEPNKSEVMNIIGEESVKNHNCI
                     LIDDMIDTAGSITQAAHALRENGAKDIYCIATHAVLSENATEKLKKADFKEIILTNTI
                     DISEKKLLPNMKILSMAPLFGEAIRRIHNGESVSSLFVR"
     gene            complement(18183..18932)
                     /locus_tag="PLV81_09480"
     CDS             complement(18183..18932)
                     /locus_tag="PLV81_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOM10367.1"
                     /translation="MDSNCKAIVLAAGKGVRMQSEMPKVLHHLNGKPLVLHVIDNLIH
                     AGLKEEDIIVVVGYRGEDVIQAIGNRARFVWQKEQLGTGHAVMQAEPVLQAYNGIVIV
                     ACGDVPLIKPETFSMLFSTANDPKTGAVVLTMELENPFGYGRIIRDDNGNLQRIVEEK
                     DAYPVQKAIHEVNTGTYAFKSQLLFKGLKTVTTENAQREYYLPDALTYIRSTGNTVKI
                     IKLHDPIEGSGINSKEELEKLEDIISKKSNN"
     gene            complement(18958..19029)
                     /locus_tag="PLV81_09485"
     tRNA            complement(18958..19029)
                     /locus_tag="PLV81_09485"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18995..18997),aa:Gln,seq:ttg)
     gene            complement(19060..19950)
                     /gene="ispE"
                     /locus_tag="PLV81_09490"
     CDS             complement(19060..19950)
                     /gene="ispE"
                     /locus_tag="PLV81_09490"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOM10368.1"
                     /translation="MIDSIKAYAKINLHLEVLNKRPDGYHNICSVMAKVDIFDLLQLE
                     QYEVCKPGVVTTSLQCTGGRNASVMDNIPARENLIAKAVEMYCYHAGIGLQVAIAIQK
                     NIPSQAGLAGGSTDAAATLLLLNNKLKHFNEQELMAIAHRIGADVPFCLNGNVALCEG
                     IGEIVTPLPPIALPASIIIVYDEIHCNTKQAYADLKRGTHPVASCKDLILSLWTKGDS
                     QTLLKIFKNDFQNIIFNNNPGLVTVYNTLKRFQPDFIQMTGSGSAIFALFFDAEHAEN
                     VCNQIQTQFSQVFFTKLLMI"
     gene            20135..21529
                     /locus_tag="PLV81_09495"
     CDS             20135..21529
                     /locus_tag="PLV81_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOM10369.1"
                     /translation="MQILMKILMAMMYLLPKKVRDNFPAIMMQKGRVAAGKNHPTIAM
                     NITNMLICTKNCGTCPSYPGIRGEALFCASGPSKGEVIENGCNCITCPLFDRCSPYSI
                     GYFCIHGQCSPKDTRSAIAKFTDMAKAYLQRFTFQNDNEEEFILEDAVKAEIIDEPDA
                     HDVTLKFIGEKEVNSKSNIPILYASLNAGIPHTHVCGGRARCSTCRVIIEEGLEYCRP
                     RNERETRLAKIKGFSPEVRLACQTTVEGNISLRRLVLDEHDISDAIRQGRGDINDVGR
                     EADVTILFSDIRSFTAFSEKALPYDVIHILNRYFETIGGIIDVNGGYIDKYMGDGIMA
                     IFGLDKEMKDDHAYLAVKSSLEIQHAIDGFNDYLSQHFNHKFKAGVGIHSGTVIIGNL
                     GFSKKKEYTALGDTVNTASRIESLNKRTGTAILVSESSYHRCQKNFTWGRKFKTKVKG
                     KEEPIVVYEVIDFM"
     gene            21884..22969
                     /locus_tag="PLV81_09500"
     CDS             21884..22969
                     /locus_tag="PLV81_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10370.1"
                     /translation="MKYIIFFIVILHGLIHVLGFLKSLGYTIPQLPAISKLTGIVWLM
                     ASFAMVATAFLYITDNDMWLLTGTIAILVSQVLIVTSWQEAKYGTLPNIIILIVVVGG
                     CGIWFFNHQVEKEIQVMLAQEAYSEKPAEKIITENMITSYPAPVQRWLRYSGVVGKEM
                     VSTVYITQKGEMRLNPEQKQWFKSYSQQYFTVTIPSFIWKVSMNIYLLPVIGRDMFVD
                     GKGEMKIKLIGLIPIAVAGDNFKIHQSALQRFLGEISWFPQAALKPYIHWEAIDESSA
                     RATITYRGVTGSAVFYFNSKGELIKFVAMRYKDINDDKPTEWVATVKGYGEFHGIKIP
                     TTFDATWMLKDGPFTWFTFTITDVRYQ"
BASE COUNT         7020 a         3973 c         4641 g         7758 t
ORIGIN      
        1 atcaggtcct tttgcaacac acgcaatgtt ttgacttctg agggcagaat tgctgagtac
       61 tgacgcattg aggggtcttc cacagaaatc atggatgatt cccatattga ccgggtatta
      121 ttgttattgg tagccagttc atataagtct tctatatcag gttttatgtc atctaattct
      181 ttatcaagct catctgtcag ctgctcaaac cgtataagct gtgaccgtat atctttataa
      241 tttgacagta ggcgttcctc ttcacgttta acagcagcat tttttattat ggcatacgct
      301 gaagtaacga taactatcat catcaaagaa ctgaaaaaca gaatggtgaa acgagatatc
      361 tggaaattaa atattttctg ttcattatga ggaatgaaca tgatggtcat tttttcatgg
      421 ccatgttcaa ggaatttatc ccacttttta gaccattgtt tctttttcag ataatatttt
      481 tctttgagct gccgctcaat atcgctcttt agtattttta gctttacttt catgattcct
      541 ttagtaaata gcactctttg tatcaggtaa tttagggcaa tggttaaaaa tatacaataa
      601 attgcaataa aaaataaaat atgctattat tgtattatat cgtaaaaaaa gcctgattta
      661 tgcaagtttt ttttatgcac ttcaagcttg aagctgcata gctaatggaa tttggtaaac
      721 atatgcaatg taaacgatgc ggaacatgtt gtaatgatgt aaggcttgcc gagtcgcctg
      781 aaatgctaaa gaaagcatat gaatactggc tgagaatgcc ttctgttgac cctaaatttt
      841 cagagatata tcttatttac cccatgctga cgtttattta tgaaaaccag tctgaggatt
      901 tgccctacca ttattcttgc aaacatttca cgcgcgatag caatggcctg ggtgtatgta
      961 gtatttacga aataaggcca cggatgtgcc gagatttccc atattatgaa ggagttgaac
     1021 ttgctcctga agacaatgta agtccttatc agggttgcgg gtataatgaa taagggtaaa
     1081 ataattccag aaaaatgctt gtattattaa caaatgtaat atacggtact catagttgct
     1141 tggtagagtt tcaccatgat agctgaagat aaaaaaaata agctccttgc aattgtgttt
     1201 tattcaatga ttgcagccta ttcctacctt gtactgacaa taggttcagg gaatgatatc
     1261 cttttctggt catatgaaga gtttattttt ggcactgtca tcagtattat actggcatct
     1321 gtaacgtact ggcttatacc cataaagatt acaggtaggt tgttaaatcc atttttttat
     1381 cttgggttaa tagtatattt cgcagggcct tttttcatag cgctgctcat agctaatgtt
     1441 gaagtgatgt accggattat ttccgggaaa attaaacctg ccattgtaaa agttgaccct
     1501 gaaatacagg atgagatggg ggtatattta ctggtaaatt ctattaccct gtctcctggc
     1561 acgctgacga taggcactga tcctgaaaaa agaaaattat tcatccattg tttgtactgg
     1621 aataagagca aagattatgc ggctgtccca aaggatgttg caggcttttt acactatttt
     1681 ttaaaaaagc ttttcaggta aagcggatac gcacatgtat gattttaatt ctatattcct
     1741 gttgtctgcg ctgttatgca tggtgtatgt atgtatcatt gtaatccgta ttgcattcag
     1801 ccataatgtc cccgaacgaa tagtggccct ggataccatt aatacattaa ttattgttat
     1861 catgattgtg ctgggcgcag ctcagaaaaa agccttatac attgatattg gtattgtata
     1921 tggaattatc tcgtttatcg ggacattata tattgcccgc tatctcatag atgagaggaa
     1981 gtaagtggat actgttatta tatcgtgcct tgttattggt ttggttgtca acggtatgag
     2041 cattattggg ctcatgcggt tccctgatat atatacccgt ttgcatgctg ccacaaaaac
     2101 aaccacgttt ggcagtatct ttgttgtttt ggcgatagtt attaagaata taatctatta
     2161 tgatcaggca agtcttacaa ttgccatcca cagtattgtt gcattgctgg ttattatctt
     2221 taccaatcca atcagtgccc acgcaatagc acgagcatca ttgctatcgg gcattaaacc
     2281 gtttggtgtt gttataaatg agtttgatta taaatcccag attaagactg cagatgagct
     2341 ggaacttgaa aaggtaagcg aaggaattcc tgatgaggat ggtgtactat gatgtattac
     2401 ttctttctgg tgatttcaat tgcactggtg atcacagcat tgctttcaat ctattttaga
     2461 gatcttctgg cagctatagt aagctattcg gttttttcac tggttttgac ggtgctctat
     2521 tttctgttgg atgcacctga tgttgctatt gcagaagctg gtattggagc agcactcaca
     2581 acctgtattt ttgttattgc catcagaatg acacggagaa gggaagaatg aggttattga
     2641 gcgttgtagc tattgttata ctattcggat ttctttttgt tggaatatta catctgcatc
     2701 cagttggaga agctttagga aattcagaag tgttacaggg aaggccagaa ctgggtattg
     2761 atatcaatgg tgttagcact atggatgatt actttttgcg caatggtcag attgaaacaa
     2821 agtcaaacaa tattgttgca agtgtggtat ttgattaccg gggatttgac acattagggg
     2881 aagccacggt gttatttatt gtggtaagca gtatttcaat gctcttcttt acatttttaa
     2941 aaagcaaaag cattgttcca gcaggtgttc ctcctgcagg tcaattccca aaaattgaat
     3001 cgagggttat tacatttgga tcttttatca tgtatatcat gatattaagc tttggggttt
     3061 atctggtggt tcatggtcat atctcaccgg gaggaggttt ccagggagga tcggttatgg
     3121 cttcggcaac agcactttta cttatcagct ttttgattac ccgccatatg caacggactc
     3181 gtaaaatttt ctcgttcttt gaatcattgg gattaaccat ttttattgga ttgggatttg
     3241 caggtatcac actatcgttt ttatataatt ttcttgctca caccagtact ggatttggca
     3301 ggattatagc ttttggacca aatcaaggct atttaatatc ggcgggaata ttaccgttgc
     3361 tatcactggc tgtaggtatt gaggtatttt ttgggttaag ccttatcgtt gtaacattat
     3421 ttcatgtatc aggggtaaaa gatactaatt cattacggta attattatgt cagcaatcgt
     3481 gctttatgta acatttattc tgttattaac cataggtata atagcactgt tatttaaacg
     3541 caatcttatt aaaattgtac tggcatttaa tatcctggat tcaggtataa acctgttttt
     3601 tatcgcttta ggataccgtg aaaatgccat ggccccaata ttcacactgg cgccattaga
     3661 gtctgttata actatggtat tgcccacacc tcaggcgcta atattaacca acatcgttat
     3721 tggatttgct accactgcac tcatgttagg agtttctgtg cttacataca gaaacaacaa
     3781 tactctggat acccggaaat tgcgtggagt ggaagagtat gattaaacat gttcctgtat
     3841 tactggtagc tatcccactt cttgctgctt tcatgatacc aatgattggt atggttaatc
     3901 gtactgcacg tactgttgtt ggggcgatag ctctggtaat gatactggca gcatcatatt
     3961 atattgctgg tgatatcctg gttcacggta gaattttgta ttacatggtt ggatcatcca
     4021 atccagaaat gttaacagaa catggattga cattcccaat acgcatagga ttaaaaatag
     4081 acccattttc actttttttt attataatga caactactct tgtgttaatt ttttatgtat
     4141 tttccgccca atatattgaa gaaaatgaaa gaaaaaatta ttttaccgtg ttattcataa
     4201 ctatggtggc aggcatgatg gggttacttg ttgcaaatga tctgtttacc ttctttgttt
     4261 ttctggaaat cctttcaatt tcatcagctg ctctggtagc attcaggact gaacgaaagc
     4321 atccgcccta tgctgggtat aaatatatgt ttgttagcgc tgtggcaacc agtttctttt
     4381 tattgggagt tgccttcctg tatgcacagt atggttcatt caacattgat tatctcaaag
     4441 aagctatacg gggaacgctg ctggataaaa ttgccattgt cctgctcatt ataccgctgg
     4501 caatgaaatc aggtgttgta cccatgcaca tgtggatacc ggatacctat tcagaagcgc
     4561 ctgcgccaat cagtgcaatg gtaaagttag gaagtttaat ttgtctgtat ggtttattcc
     4621 ggatatcatt ttcgtttggg atgagccatg cccatgtgag ttaccctctg ggtgtattgt
     4681 ttattgtttt tggtatattg tcaatgtttg tcggtgtgac tcaggctctg gtacaaaaag
     4741 atgtaaagcg cttgatggcc ttccatgcag tttctcaggt ggggtatatg cttcttggta
     4801 tgggtgttgg gttgacagta gttaaatcag gtgacgcttc ctttgctctt tttggaaaag
     4861 tggcaatgat tggtgggttg ctccatgttg ttaacaacgc attgtataag ggattgctat
     4921 ttttaacatc gggcattatg gtaaggcact tcaaaacacg taatttagat gcaatgcatg
     4981 ggttggcgca ttatgacgtt tttactacgg ttgtatttat gatagcagcc ctggctatat
     5041 cagggattcc accgttcaat ggttttgtgt caaaaattat tatctatgaa tctgtattcc
     5101 agtataatcc ggtattgacg ctcattgcaa tctttgtttc aattttaaca ctggcttcat
     5161 ttatgaaagt attttacagt gcattcttag gtatgccttc aagccgacga ccaatggaac
     5221 agccggtact gatcaagtgt agtatgtttg ttttggctgt ttttatagta gttataggta
     5281 tatatccgca gggtatactg aaggcaataa ttgaaccagc ggtaaatgca ttaattaatt
     5341 tttagggata cagtgatgaa tatacttcag gttgaatatc ttcattgggc gccattgttc
     5401 tggttaggaa tttttattgg attagctgtt gtagtggtta ttattggcaa actgtttttc
     5461 agaaatgatt ataatacagt tgatcagcaa agccttcctt tctattcagg gacaagcaat
     5521 cccctgctgc aacttattac cgcaagcagt ttatactggg gatttaagca catgctggat
     5581 gcttattttt tgattataaa aagattattc ataagtgatg ttgaagattt tattcaatgg
     5641 tttattatca ttgttgcagg tatgcttctt attctgactg gggtattagt gtgaaaaatg
     5701 taatttaccg gcatttgaag cgatctttgt gggcttacca tatgaataca gggtcatgca
     5761 atgcatgcga tattgaaata ttagctacac tcatgccacg ttatgacgca gagaggtttg
     5821 gtataaagct ggttggcagt ccccggcatg ctgatgtgct gcttgttact gggaccgtaa
     5881 ccgaaaaaat aaaagataat atacttcaag tgtacgagca ggttcccgaa cccaagctgg
     5941 ttattgttat tggaggctgt ggctctacca aaggggtgtt ccaggaatcc tatgcaatgg
     6001 ctggcccggt cgatgcaata ttacctgttg atgtatatat accggggtgt cctccaaggc
     6061 ctgaagctat catatatggc atagcaaaag cgtgggaaaa actggaaaaa ttacaaaaat
     6121 gatgttatgg tatatatgca atgaaaactg aatccatagt aacggtatta cagaataaat
     6181 taggaagcca catacaggca tcgcgtgaaa agattaccaa ttatggtaat gggattaacc
     6241 gagtaacact ctggatcagt attgacaggg agtcttttca ttcctcaata gcaatcctga
     6301 aaactatggg aaggctgcat atatcaaccc caatgcccta taaagagtat gaagaccgca
     6361 ttgagcttat atatccattt gcgttgaaag aaaaagatac aaagttttct gaaatcatgg
     6421 ttattatctc ggtggatatt cccaaagacg atttaaagat aaggacaatt actgatattg
     6481 ttccgggagc cctttttatg gaacgggaaa ccatggaaat gataggtgtg gaaattgaag
     6541 atattcctga cccaagaagg ctttttacgg gcaactatct ccctgatggt gtttatccat
     6601 tgaggacccc aaaaaataag gaatcaatgc aatgaaatca gatataacgt atcccttgaa
     6661 tataggtcct gttcatccag ctttcaaaga gcccattaaa ttcacgttcc ggattgaagg
     6721 agaaaaggtt gttggggctg atatcgattt tggctacacc catagagcaa tagaaagaat
     6781 tgcacaggag agaaactaca tacagataat ctatcttctt gaaagggtct gtggaatatg
     6841 ctcattctcg catcccatgg catattgcct tgccatagaa gatgttgcag gcatagaagt
     6901 gccgccacgt gcgcggtata tacggactat tgttggtgaa ctggaacgcc tgcactcaca
     6961 tttattgtgg acaggtgtag cagctcatga aattgggttt gatacacttt ttatgtacac
     7021 atggaatatc agggagaaag tccttgattg tttagaagag ctaaccggaa accgtattaa
     7081 ttatgccatg cttacaattg gtggtgtacg acgagatata actgcagaca atgcacagac
     7141 tattcatcag gtgctggaat actatgaaga gttgtataat agaacgtcgg aaatattgct
     7201 tgatgattta acgctgagag ccaggatcga aggagtagga attcttactg cagaacaggc
     7261 tgagcgatta tgcgcagtgg gaccaacggc cagaggttca gggttgatga aggatgtgcg
     7321 ggttgattat cctgtgaatg cctactatga aataccgtgg ttgaagccag tatccccacg
     7381 ggatattggg aaagaaccaa ttggtgatgt gtatgacagg atggctgttc gagtgctgga
     7441 aatacagcag gcaattaaaa taatccagtt ctgtatggaa aaccttcctg aaggggacat
     7501 taacactggc agtggtgccg ttaagatgat caatgcactg aagaagttag aaggggaggg
     7561 agtaggacgg tatgaagctc cccgtggcga ggtatgccac tatgttatac tggataatca
     7621 ggaacatccg gttcagataa aagtaaaggc gccaacatat tccaatgggt ttacctgggc
     7681 accaatgctt accaatattg agatagctga tatcccaata gtcgttgctt caattgaccc
     7741 atgtgtggcc tgtgctgata ggatgacata tgtgcaagca gatggaagcc acactaccat
     7801 atcgtgggaa gaattgcgtg caaaaagtat acagaaatat aaaggggtac gcagacaatg
     7861 gatgaagtga taggtgtggc gatagctatc gtagtcgcag gtgtatttgc ggggcttttt
     7921 ctgaaaggga ttgatagaaa acttgcagct tttatgcagt caaggattgg gccacctatc
     7981 gcacagccta tatatgatat tttgaaatta atgcagaagc aaacaattat cccgtcaaca
     8041 tctgttcggt ggttgtttac cggcgcacct cttgttgcac ttacttcagg cattgtgtta
     8101 ttactctata tctccctttc ttatgtgtgt tatataatcg gattacaaag ccctgtacag
     8161 ggtgacctga tagttattat ttatcttatt cttataccat ctattgctat tatcagtgga
     8221 gctttcgctt caggatcacc ctatgcttct ataggtgccc agcgtgaaat ggttatttta
     8281 atgagtaccg aattacctat cgctgtggtt gtgttgagca tagcgtggca tgtagctgat
     8341 agtatgtata atccatttgc cttgcaatct ctgtttgaat atacgttatg gcaccatgct
     8401 ggtgcatttg ggatccttgg tggtattttg ttgtttcttg caatgatact tgtcctacct
     8461 gcagaaatgt ctaaggtgcc atttgaccag gcagaggctg agactgaaat agcggaaggt
     8521 gtgatggtag agtattcggg gaagttatta gcatttttgc tattaactga tgcgtttaaa
     8581 gcattgtcat tatcggcttt gatagttata ttatttttcc cctatacatt tacaggtttg
     8641 tttgggttat ctttttccat tggtagttat agtattactc cactgattga aataatcttt
     8701 tttataggaa aagttgtcat tgtatatagt attggagtaa ctgtaataag ggtaattatg
     8761 gcacggttga agatttctca ggtagccaag gtgtttctat ttgcagtttc tgctataagt
     8821 attattggaa tggtgctcat tatgttagat ccaaagatga aaatgatatg aaaggtgagc
     8881 catgttagga atggtatata aagtggtaga gcagttgttt gcaaaaaaat ctacaaatta
     8941 tttcccttca aaatatatac tggaggatac agcagatact cttaaaaaag gtacaataca
     9001 tccacccgtt gcggtgaaag aaggtttcag aggcagatta gaatataatg ttgaaagttg
     9061 taccggttgc ggattatgca gcaaggtatg ccctgccaat gctattgaat tatatcctgc
     9121 tgtcattaat gataaaaaaa caaagcgaat tgtgatatat ttatcccggt gcacctactg
     9181 tcaggaatgt gttaatatat gtcccaaaaa ttcaatacag attactacac atttttttat
     9241 ggctgattac aataaatatg atgcttcact ggttgttggt ttggaacaca gaaagaaata
     9301 tgaagtaaaa gaagaaggat caccggatgt tcaaaaagaa ggaaattgaa ataaaggtta
     9361 aattgtatag cggattacat aaagatgcca gcattgacag atataacccg gatgaagggt
     9421 atatactgaa aattcctaag ggttcccgcg ttagatatgt ggtaaaacaa ttaggattgc
     9481 cggatatcta ttcgttggca tatttttgta atggacagcg aattgggatt tttacaaagc
     9541 ttcgtgaagg tgatgaactt tcttgcttca ggccttccgg cggaggttga tttacaaaag
     9601 atattcatca tccctgtgac gtaatgatct gaaaatgctt gattgtgcac cttttaccga
     9661 tttattaata atttttaata gttcttttgt ctgctttcgc ctggttgtat ctttataggg
     9721 gcagtcattt tccaaaggta caagcggtaa tgcattgcaa taacctttca cttctgcttc
     9781 agtgagaaat gcaagtggcc tgattatctc taatgcgcca ttgaacattt ttaattttgg
     9841 cggcattgtc gatagtttcc catgatagag catattcata aaaaatgtga cgataatatc
     9901 atcctgattg tgaccaaagg caattttttt gcactgaagc tgggcagcag tttcaaataa
     9961 ggcttttctt ctgaaccatg aacagacaaa acatggtgat gaattggtat cagaaagatc
    10021 taatgttatt tcttttacgt ataaaggtat gctgtaatgc tggcagtatg tcctgagtgt
    10081 ttcaatagtt tctttgttat gaaagtttgt aacagagaca tggcagcaat acaaattata
    10141 tgtaatggga atgtgttgta aacgacgatg taatgcatcc atcaaaacaa gagaatcttt
    10201 cccgcccgaa agagcaatga gtacattatc attagaactg atcatgctgt aggtatttat
    10261 ggctttacca acaactttta aaagctttgt aatgtgtttg tttgtgagag tactcataat
    10321 aaagccttga cgtaaagatt atggtatgat taagtagtgc ttggaaagga gtagttgtca
    10381 atagaatttg tacgtaatta tcatagttaa tgcagaggtt ttgtaatggc tcgttgtatg
    10441 atagttgatg attcaagttt tatgcgccgt attatcagac aaacattgca ggaaggtggt
    10501 catgaggtag tggcagaggt tgataatggc cttgaagccc tggaaaaata tcgagaaata
    10561 cagccggatg tagtcacaat ggatattacc atgtggggta aagatggcct tgaagcaata
    10621 tctgaaataa ccgatagtta cccggatgct aaaattattg tgatatcagc attaagtgaa
    10681 agaacattga aaataaatga gaaggatatt aaagcccgtg tgtttttaac caagccgttt
    10741 gaaaagaaag agctgttaga ggcaatcaat aaagtcctgt aacatggaag agcaattatt
    10801 acaattatat aaaaaaagta ctgtggcaga tagcgcatat ttaattgccg acggcaaggc
    10861 atatttattt gttacagaag ctgataagta tcttttacag ggtaaaaatc ttattattgg
    10921 tgccactgaa ctggtattgg gtgcaaaaac taatgaaaat atattgaggc ttgaaacagc
    10981 attagcgctt aaagggacaa caataaaaaa aattcctgct gcaacactta tacagggatt
    11041 ggacaatagt gcgtttgcta tgaatctggc aattgttgaa gccaaaatgt tggtcctaac
    11101 caatgaaatc ttgaaaaaag atttgcaact ggattcacag cttgtaaaag aaaaaaacat
    11161 atgtattgaa tattttacaa taattcaatc tctccaaaaa gaatatgata aaagaaggct
    11221 tccatggatc aaggaactta ttgaaaaata taaaaccagt ttgatatata aaaaaggcga
    11281 agcatttacg cgggcaacag aatcactaaa agttgaaacc ccccgggagc tttcttcaaa
    11341 cactatagaa tttcctcctg gttctgtttt atgtcaggaa ggcagcattg gtgattttat
    11401 gtatatactg gaatcaggtc agctcgaggt gtatatacag aatgcaaaag ttgccacaat
    11461 agctgaaaaa ggaacagtca ttggggagat agcaatgtta ctatctatgc cgcgaactgc
    11521 cacactaaag gcccagaata cagttctagt tacaaaagta accagggatg atttgaaaca
    11581 atcttcctca cctcagctta tacagatgtt actggtttca cttgccaaga aacatcaggc
    11641 aaatgttatg catattgaag atattaatga gaaaaaagct cttgcctatg cagaaaagaa
    11701 agagcaagag cctcataaaa ctgatattca ccgttatatt aatgaattat cgcagcttaa
    11761 aagtggatta gaaaaactaa tcaacactaa acaggctgat tatttagaat atatactgaa
    11821 aattaaataa aaattattca acctcagtta atacttcacc tttctttaat gatcgcagcc
    11881 agttgagctc agcttttaaa tgattttcca gatatgtgta cacatgtaac tgtgatgcgg
    11941 gtgatgtatt cttagattca gcttcaatct tttctcgcaa tgcattcatt ttttctttga
    12001 tatgttccag acgatcttca atgaactgtt cgcgctgttc tggtgaaaga gaatttaaaa
    12061 acatcaacac tatatcaata tcaaaatgta taaaattgtg gtcaaagtac tgttttaaca
    12121 tcttcttgta atactttctt ccatccggta atatctggta tatataccgc tcaggatttc
    12181 cgccttcttt ttccgttcca atttttttaa cccagccatt atccagggct ttttttattg
    12241 cataataaat agacccaaat ttgatatcaa cataatcttc aaggcgttca actatccttt
    12301 ttttgatttc atagccataa agattttcct ccataaggag ccccaatatg gtaatttcga
    12361 ttttcatatt tacctctcta taaaaattaa attatttctt ttaaaataca atgaagattg
    12421 aaaaaaagca ataataatat tgtatgttta taaaaatatt ttaaaaataa tttaaattag
    12481 attatataaa tatgacttta tatatagtta catttaatat agtttaattt tactattttt
    12541 attatatcac aaatattctg gattatttag atagttgttt taatgacaaa cggataattg
    12601 tttcaatatt gtttatgtca ctatgttgtg caatgaccga tgaaacagta gatgaagcct
    12661 gcttttcatc aaatcctaac gataccaatg cttcaatagc atctttcata tatggtgttg
    12721 tagtagattc ccgggacagg attgtatcta gtttttttaa ttttcttttt aattcaaaaa
    12781 taattttttc acttttgctc tggcctatgc ccggtatgga tgtaaatgct gttatttggt
    12841 cattttttac agccgatagt atctgctgag gagtcatgga tgataaaatt gaaattgcta
    12901 ttgacgggcc tattccagaa atggtcagca gaagtgaaaa tatctgttgc tcttcttcgg
    12961 agttaaatcc atacaacaat atggcatttt cccttacaac catatgaata aaaagctctg
    13021 tacttctagc cgtactaatt ttttcatagg ttgacagggg aatatgaatt tcatagccca
    13081 caccctgaac atcaagaaca accctggtag gtttacaaga aagtatattt ccctttaaaa
    13141 acgatatcat agtatgttat aataaaccct tttactatta aatatttttt ttgattattg
    13201 agtaacctgg taaagctaaa cagtgacata atgcaatggc aagtgcatca aatgcatcat
    13261 catactgtat gatttctgga gtttttaata tttttttaac catgaatgca agctgatcct
    13321 ttgaagaacg cccgtacccc gttattgatt gctttatctg tacaggtgta tattcagcat
    13381 acgggatatt gtgctgagcc agcaacaatt ttatcacacc aataacctgt gcaactggaa
    13441 ttgctgtttt aacattttta ttgaaaaaca atttttcaac tccacaggat accgggctat
    13501 atttttgtat gatatctgaa agtgaagtat atacatctaa caaacgattt tcatcagtgg
    13561 gctttgtttc gataactcca caatcgatat atgaaaaaga atcacttatg atagcccagc
    13621 cgcaacgccc atatcccgga tcaataccaa gtaaataagc cacgtaatat caactcatat
    13681 tgttaataat atcatcaggg atatcaaaat tagcatgaac actctgaaca tcatcatgat
    13741 cttccagtgc ttcaatgagt ctaagacatt gcgaagcttt ttgcccttct aactgtactg
    13801 ttgtctgggg aacataggta atttcgctat tcagagttgg taaattattt gaacgaataa
    13861 cttctaaaat atcattaaac ttttcagggg cagtgtaaac aacaattgta ttgtcttctg
    13921 tcttaatatc ctcaatatca taatcaagta aaatttccat gacgttgtct tcggttgttg
    13981 cgtttgcatc aatgtttata acgccttttt tatcaaaaag atatgcaacg catcctgttt
    14041 cacccagatt tccgccattt ttagaaaaaa tagtgcgtat ttcaggagta gttcggtttt
    14101 tattatcggt catacactca accattatag ccactccacc aggtccatat ccttcatacc
    14161 gaacttcttc atatgttgag ccttcaaggg cgccagtacc ttttttaatt gctctttcaa
    14221 tgttatccat tggcatatta ttttcacgag cttttaatac agcggtacgt aatctaggat
    14281 tactgttcag atcatcgcca ccaacccgtg ctgcaaccgt tatttcgcgt atgagcttgg
    14341 taaatacctt accttttttg gcatcctgtg ccgctttcct gtgttttatg tttgcccatt
    14401 tagaatgtcc tgacatgttt tattctcctc aatgtacaag ttttctagtg ttattacaaa
    14461 gcaagaacga atggtagaat catgactaat tttgtcatta ataaaataat cacaaaattt
    14521 gtgtaaaaat aattttttca ataaccacgt gaaattttca accgtatttt ttcaattttt
    14581 tttgaggatt cacggatttt tgtacctgaa gcgatagctc tggtatacca gtctattgcc
    14641 tgctgatatt gcatggtatt ttcatacgtt gaagcaattt tgaagtacag ttcccctacg
    14701 gtaagtttgc tatattcatg ctcaaggcgg cgtaattcta attcattatt tcctaattca
    14761 tccataagag actggtattc ggggtagagt gatatgtcct ttttatcaag tagtgtagat
    14821 ttaatggtgt taattttgat tttgatccct tcaatactat tttttttatc ttctatggac
    14881 tgatgtattc tttcagaaat ttgtatagcc tggagatagt ggttgagact ttctgtgtaa
    14941 tttaaatcca tactggttat atcaccaatt attgcatgtg atattccttt ccttatcaga
    15001 tcactatcgc catagagtga cagccctttt gatagtgagg tataggccaa tttcatgtct
    15061 gaaattttag tataggctaa atacccaaga gcaaattgta tagctgcatc atcagggtgg
    15121 atagtaagat ataattgata atagcgtcct gcgtttataa agtcacctat agattcatag
    15181 cagtttgcag cagttaaata aagttcagga ggggtatcgt tgccttttgc ctgtatataa
    15241 cggttgagat gaaaaagtgc ttttttcttg tttttaccag aagcaagcgc cagagctaat
    15301 tctaatgagt aatcataatt atcaggttta atttcatatg cttcttccaa ataagtcaga
    15361 tatgcgtcat atttattgag gcgtttaaaa aggagagcaa gggaatagaa gattgtatgg
    15421 tctttttcac ttatggtggt aaatcgtaat gcctggaagt aatgttgtac tgcttctttg
    15481 tatttgaaca tattaagata gcaaagggca gcattgatat gtagtgtata aagtgtatca
    15541 ttattttcta taaaatatgg ctgtaatttt ctatcagtaa ataatttttt caagtctatg
    15601 ccctcttcta tttttccaat tgcaacatat gagtcaggaa cctttttatc aatacttctg
    15661 gaagaataca tttcatctaa ttgcaattgt attatctgca gggcatcggt gaatttattt
    15721 tgctgtatca gagcaacata atcctgccca ctgccattat caatgagcag caatatggaa
    15781 aatatagaaa ggtacaaaat tagcgattta aatttcataa tagttgtttt gactgtttag
    15841 tagtattatc ggatggtttg aaaagcaagt tgaattacag gaattatcgc cgaatacgta
    15901 ttttcggaag ttgacgtttc ttgatttttg gggcgccttc tggattatat ttattctgaa
    15961 catcttcaat gctatgagtt attagcattc tgaccacttc ttcagctttg ttaagtacat
    16021 ctattagtat aaaattttca tcttcagaaa aatcattgag aagatgttct tccatggtca
    16081 cgcctttttt aggatagcca atacctattc taaccttagc aaacctgtca gattttaacg
    16141 cgcgggtaat ggattcaatt cccgggtgtt ttagctttga catgatggag tctattcgta
    16201 tctcgccaag tggcagggat gggtcttcca gcacacatat gatatcacgt acattaattc
    16261 gtagaaaaga agcaatgtaa agaacagact cacctaaaag ggtaacaaag gtttgaggtt
    16321 ttagcaaaac aacatcttcg ccagaaatct tgccacggcc tataattgat tttttctttt
    16381 taatgcggat gtcgatattc tcgttattac caagaatatc gacaacctta aatccaatgt
    16441 tttgtctgtt gttaaaatat tcttcgcctg gatttccaaa tcctacgatt agcttcaatt
    16501 attaactcct tatattaaat tgttattgct gttcttcagc aggctgaact tcttctgcaa
    16561 ctggctgtgc aacttcttca gctgccttat gaggtgcaag aacagctaca attactgtct
    16621 caggggtact aagaattgta acacctttgg gagcagcaat atcctttaca tgtatagatt
    16681 gcccaattga aagatttgtt acatcgatag taattttttc tggtaaatct gcaggaagac
    16741 attcaacctc aatttcacgc tcaataattt caagtatgcc gccctgttta acaccaatag
    16801 aagttccgct aatttccaca ggtacttttg tagaaatagc ttcggtcatg gttactctaa
    16861 aaaaatctac atgcagtatt tcgtctgtta tgggatgtcg ctggtaatcc ttgacaaagg
    16921 ctttaactgg agagccttta gaatctttaa tgtcaaggtc tataaggaca ttttcggata
    16981 tatggccttt aaaaatttta aagaagtttt tcttctgaac cattatggtc tgagattcac
    17041 catgagagta caatactgca ggtatatacc cctgtgctct gagcttatga tttgcatttt
    17101 tccctatctg ggttcttgtt tcaactggta aaacgtgtgc ttccatatca acctctttta
    17161 aattatttaa tatacattat agtgaaaatg ttataaaatt caataataaa ttttttcaac
    17221 tatcggacaa acaatgaact aactgattcg ccgttatgta tgcgacgtat agcttcacca
    17281 aacagtggag ccattgataa tattttcatg tttggcagaa gtttcttttc tgatatatca
    17341 attgtattcg tcaaaatgat ttctttaaaa tctgcttttt taagcttttc agtagcattt
    17401 tcagataata ctgcatgcgt tgcaatacag tatatatcct ttgcgccatt ttcccgtaat
    17461 gcgtgtgctg cctgtgttat ggaacctgct gtatcaatca tatcgtcaat taatatacag
    17521 ttgtggtttt ttacactttc ttcgccaatg atattcatta cctcggattt attgggttca
    17581 ggccttcgct tatctactat cgctaattct gcaccaatat tccttgctaa aaaccgtgca
    17641 cgttcagttc caccagcatc cggtgatact acggtgggat ttagtatatt gagttctcgt
    17701 acatattcaa tcaccagtgg cgatgcaaaa agattatcca ccggaatgtc aaaaaagccc
    17761 tgtatctgat cagagtgtaa ctccatggta agaattcggt ccaccccagc agcctgcaac
    17821 atatttgcaa ctaattttgc tgaaatggga acccggggtt caacctttcg atcctggcga
    17881 gcatagccgt agtaagggat aacagcagta attcgctgtg ctgaagctcg catggcagca
    17941 tccaccataa gcagcaattc catgatgtta tcgtttgcag ggttactggt ggattgtatt
    18001 aaaaaaacat caacgcttcg cacgttttca agaatcttga cagatatttc cccgtcctta
    18061 aattttttaa tttcaatttc tgaaagttca atgcctaaat attgtgctat ttcttgggct
    18121 aacgggaaat ttgaagttcc ggaaaatagt tttattggat atatcatata aaaccctttt
    18181 tgttaattgt tacttttttt agaaataata tcttcaagtt tttctaattc ctcttttgaa
    18241 tttataccac tgccttctat tggatcatgt aatttaataa ttttgacagt attaccggtt
    18301 gaccggatat atgttaatgc atcaggaaga tagtattctc tctgtgcatt ttcagtagta
    18361 acagttttta aacccttgaa aagaagctgt gatttaaatg cataggttcc tgtattaact
    18421 tcatggattg ctttttgcac agggtatgca tccttttctt ccacgattct ttgcaggtta
    18481 ccgttatcat cgcgtattat ccgcccatag ccaaagggat tttctagttc catggtaagc
    18541 acaactgctc cggtttttgg gtcattggct gtagaaaaaa gcattgaaaa cgtttcaggc
    18601 tttattaacg gcacatcgcc acaggcaaca ataacaatac cattatatgc ctgcaataca
    18661 ggttcggcct gcataactgc atgccctgtt cctaattgtt ctttttgcca tacaaatcgt
    18721 gctctgttac ctattgcctg tataacgtct tcacctctgt agccaaccac cacaatgata
    18781 tcttcttctt taagacctgc atgtatcaaa ttatcgataa catgaagaac cagtggtttg
    18841 ccattcaggt ggtgcaatac cttgggcatt tcagattgca ttcgtacgcc tttacccgca
    18901 gcaaggacta tagctttaca attactatcc atacatggta ccaataaaat aaaaaatctg
    18961 aggtgccagg attcgaacct gggaatgccg ggaccaaaac ccggtgccgt accgcttggc
    19021 tacacctcat tattagatat aatatagtaa taatttttat tatatcatta ataatttggt
    19081 aaaaaaaacc tgtgaaaatt gggtttgaat ctggttgcaa acattttcag cgtgttctgc
    19141 atcaaaaaaa agtgcaaaaa ttgcagatcc actccctgtc atctgaataa agtcaggttg
    19201 aaaacgtttc agtgtattat atacagtaac aagcccggga ttgttgttaa atataatatt
    19261 ctgaaaatca tttttaaata ttttcagcaa tgtttgacta tcaccttttg tccataatga
    19321 aagaattaag tctttacagg aagctactgg atgagtgcca cgtttgaggt cagcataagc
    19381 ctgtttggta ttacaatgaa tttcatcata gacaataatt atggatgcag gcagagctat
    19441 tggtggcaat ggtgttacta tttcgcctat accctcacac agtgcaacat tcccgttaag
    19501 gcaaaatggg acatcagccc caattctatg agctatcgcc ataagttcct gttcattaaa
    19561 atgtttcagc ttattattaa gaagcagcaa cgttgcggca gcatcagtac tgcccccggc
    19621 taatcccgcc tgggatggta tatttttctg aatggcgata gcgacctgca atcctatgcc
    19681 agcatggtaa cagtacattt caacagcttt tgcaataaga ttttcccttg cagggatgtt
    19741 gtccataaca gatgcgttgc gcccacccgt acactgcaat gaagtggtga ccacaccagg
    19801 tttacacact tcatattgtt caagttgtaa aagatcaaaa atatcgactt ttgccatgac
    19861 gctacagatg ttgtggtagc cgtcaggcct tttattcaat acttcaagat gaagatttat
    19921 ttttgcgtag gcttttatac tatcaatcat taatagcact tttattacag aatggcaaat
    19981 ctatattttg actgttttcc tgtcaagtaa ttaatagaag tagtaagata tgcaaaaaaa
    20041 ttgttgcatc gttactataa atttgtttaa aattatatat tattactatt ttatctacaa
    20101 acatttttat aatattgagg ttctaaataa tacaatgcaa atcctgatga aaatattgat
    20161 ggctatgatg tatctcctcc ctaaaaaggt gcgcgataac ttccccgcta taatgatgca
    20221 aaaggggagg gttgctgcag gaaaaaatca tccaacaatt gccatgaata ttaccaatat
    20281 gcttatatgt acaaaaaatt gtggtacttg tcccagttac cctggaatac gaggagaagc
    20341 gcttttttgt gcttctggtc caagtaaagg tgaagttatt gaaaacggtt gtaattgtat
    20401 tacatgtcca ctgtttgata gatgcagtcc atatagtata ggatattttt gtatccatgg
    20461 gcagtgtagc cctaaagata caaggtcagc aattgctaaa tttacagata tggctaaggc
    20521 ctatttacag cggtttacat ttcagaatga taatgaagaa gagttcattc ttgaagatgc
    20581 ggtaaaagct gagattattg atgaacccga tgcacatgat gtaacactaa agtttattgg
    20641 tgaaaaagaa gttaattcta aatcaaacat cccaattctg tatgcatcgc ttaatgcagg
    20701 gataccacat acacatgttt gtggtggtag ggcacggtgt tcaacctgta gggtcataat
    20761 agaagagggt ctggaatatt gtcgaccccg aaatgagaga gaaacacggc tggctaaaat
    20821 aaaggggttt tcccctgaag tcaggcttgc atgtcagacc actgtagaag gtaacatctc
    20881 attgcggagg cttgtactgg atgagcatga tatatccgat gcaatacggc agggtagggg
    20941 tgacatcaat gatgtaggaa gagaagcaga tgttacaata ttattttcag atatacggtc
    21001 ttttactgca ttttctgaaa aggctttgcc gtatgatgtc attcatattt taaacaggta
    21061 ttttgaaaca ataggtggga ttattgatgt taatggaggc tacattgata aatatatggg
    21121 tgatggtata atggcaatat ttggtctgga taaggaaatg aaagatgacc atgcctatct
    21181 ggctgtgaaa tcatcactgg aaatacaaca tgcaattgat ggattcaatg attacctatc
    21241 acaacatttt aatcacaagt ttaaagcagg tgttggtatt cactctggaa cggttatcat
    21301 cgggaactta gggttcagca aaaagaaaga atatactgca ttgggtgata cagtcaatac
    21361 agcttcacgt atagaatcat tgaataaaag aactggaact gcaatattgg tttctgaaag
    21421 ttcatatcat cgttgccaga aaaattttac ctggggacga aaatttaaaa caaaggttaa
    21481 aggcaaggaa gagcccatag ttgtttatga ggtaattgat tttatgtagt agaagataaa
    21541 taagctctaa ttgcctgctc tgatgaataa cgtggtttaa atctttttaa gcctttctat
    21601 gacacgcgaa ccaacatacc caaatgctcc ggtgattgcc aatattcgtt tttgtgtttt
    21661 cataatgtat tccttatggt ataaggttct ttcattttaa tgcagcaata taaatagtat
    21721 caataacttt ttttatattt tttatatatt gcaaatttca attaagggag ataatactgt
    21781 tttagaggta ggctcataga taaaaggtga acatatcaat aatctcaccc ttaatagctg
    21841 tatttgaaag ttcttatcaa tatgtatttt taggaggcaa catatgaaat atataatctt
    21901 ttttattgtt atacttcatg gtcttattca tgttcttggg tttttaaaat ctttggggta
    21961 tacaatacct caattgcctg caataagcaa acttaccggt atagtttggc tgatggcaag
    22021 ctttgcgatg gtagctacag cttttttgta tattactgat aatgacatgt ggttattgac
    22081 aggaactatc gccatacttg tgtcacaggt tttaattgtt acaagctggc aggaagcaaa
    22141 atacggaaca ttgcccaata tcatcattct catcgttgtg gttggtggat gtggaatctg
    22201 gttttttaac catcaggttg aaaaagaaat tcaggtaatg ttagcacagg aagcatattc
    22261 tgaaaagcct gcagaaaaaa ttatcactga aaatatgatt actagctacc cggcaccggt
    22321 tcaacgatgg ctcagatact ctggtgttgt tgggaaagaa atggtgagta cagtgtatat
    22381 tacacagaaa ggtgaaatga gattaaatcc cgagcaaaag caatggttta aatcgtattc
    22441 acaacagtac tttacggtta ctatcccttc attcatctgg aaggtaagca tgaatatata
    22501 tctgcttcct gttattggca gggatatgtt tgtagatggc aagggagaaa tgaagataaa
    22561 gttaataggg cttataccaa tagcggtagc tggcgataat ttcaaaattc atcaatcggc
    22621 tttacaacgg tttctaggtg aaatttcgtg gtttcctcag gcagcactca aaccctatat
    22681 acactgggaa gcaattgatg aatcaagtgc cagggcaaca attacataca ggggtgtaac
    22741 aggcagtgct gttttttatt ttaattcaaa aggtgaactg attaaatttg tggcaatgcg
    22801 atataaagac attaatgatg acaaacctac agagtgggtt gcaactgtta aaggctacgg
    22861 tgaatttcat ggtataaaaa tcccaacaac ctttgatgcc acatggatgc taaaagatgg
    22921 tccttttaca tggtttacgt ttacaattac tgatgtaaga tatcaataac aacagtattg
    22981 ctatatatct caaaatattg ttatgatgga agaacgttat tgaattgtga tgcaataaat
    23041 atttattgac aaaatagttt tatatgtata gtatgattga caattatatt tatattagca
    23101 attattcaaa agacattgct gatttagatt gttgataatg ttctatataa ggaaagatat
    23161 tcccctcatt atgcctcata attgcaattg tagagtattt ttatttttaa aaaatccaaa
    23221 tataacacta tgaaagcaaa accaatctcg ccatctacat tacgattgtt tggtgttttt
    23281 ttaatatgtt atgtaagtgt gtgtaatgta tgtggctgtt cgccttcgga agtatttttc
    23341 tcacctcatg ctgcaactgg tggggttatg gattgttcaa cattagattt ta
//
LOCUS       DAORYL010000047        22740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1915_length_22740_cov_12.997047, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000047 DAORYL010000000
VERSION     DAORYL010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 22740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22740
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1915_length_22740_cov_12.997047"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PLV81_09505"
     CDS             complement(<1..689)
                     /locus_tag="PLV81_09505"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOM10371.1"
                     /translation="MELTFNLPGLETIEQRTVPLDSSTLYDVLIVGGGPAALTAAVYC
                     MRKGVKTGLITLDVGGQVAETSTVENYMGYRHIEGISLVQRFKEQVEQFEIGFLQGYK
                     AKKVIDGNIKKVECEDGNIYQAKSIIITTGKRWRRLNVPGESELTGKGVAYCAICDAP
                     LFKDKDVIVVGGGNSAIEAAIDLAKLATSVTVVQLLPALTADKVLIDELSKFTNVTIL
                     YEHEIKAIHGT"
     gene            complement(908..1657)
                     /locus_tag="PLV81_09510"
     CDS             complement(908..1657)
                     /locus_tag="PLV81_09510"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HOM10372.1"
                     /translation="MAARNKGIQLMINKIEKAPDIPSMVVSQIIELISSGVLKPGDRL
                     PSELEMTRRFGISRISLREAMKLLEAKGYIESQGRKGKYVKSVVDDAIKTPIEGLIQI
                     DHKKIWELLEVRRIIDAEAAALAALRANEEQRRKLNSFRTQIELMGVENLIQKREGGK
                     FYAQFYNHLYEATNNTIFAHILNAISTILRGVLPYSREKLAKVKNTSKDIYEHHIKIL
                     DAINSHKPDMAREAVVEHIDYLERTLKKILK"
     gene            2219..2704
                     /locus_tag="PLV81_09515"
                     /pseudo
     CDS             2219..2704
                     /locus_tag="PLV81_09515"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
     gene            2753..3826
                     /gene="aroC"
                     /locus_tag="PLV81_09520"
     CDS             2753..3826
                     /gene="aroC"
                     /locus_tag="PLV81_09520"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOM10373.1"
                     /translation="MHGTNTFGQVLKVTTFGESHGKALGAVVDGLKPGIPLSVEDIQK
                     QLDRRKPGQSSVSTQRKEADSVEIVSGLFEGKTTGTPLCMIVYNKDQDSRAYESIKDI
                     FRPGHADFTFWQKFGIRDYRGGGRSSGRETVGRVAAGAIALKMLKEKGVTIVAYAEEI
                     AGIKGEKVDIDFIEKNPVRSADPDKAQAMEQAIKQAQAEHDSVGGIVACIIKGLPAGL
                     GDPVFCKLDARLAMAVMSIGAVKGIEFGSGFAAARKRGSENNDQMSDGKFLTNNAGGI
                     LGGISTGQDIVMRIAVKPTPSIAKPQKTMDIDGNTVDISIKGRHDPCIVPRIIPVVEA
                     MVALVVYDAWLLQERIGRYDGTV"
     gene            3810..4742
                     /locus_tag="PLV81_09525"
     CDS             3810..4742
                     /locus_tag="PLV81_09525"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOM10374.1"
                     /translation="MTVLYNLSERDEVFKNPVITIGSFDGVHRGHRAIFDRMLELAQS
                     LHGEPVVFTFSEHPRKLLTPHTPPKILTTAAEKIHAIEQVGINNIVMMPFTHEIAALT
                     AEEFLFKIVFYHIGKAHIVVGYDHAFGKGRQGDFEFLKGLSLREGFLVERVEPVCIQS
                     RPVSSSWIRTEIEDGNIALANMLLGRPYTLRGTVVKGQMRGRLLGYPTANITPDDPDK
                     IIPKDGVYAVRVHINGMLYHGMLNIGTNPTFENTERTIEVHIFDFDADIYGQTLEVFF
                     IERIRDEKRFGSVTELKEQLHNDKLASEKILAGN"
     gene            4745..5323
                     /locus_tag="PLV81_09530"
     CDS             4745..5323
                     /locus_tag="PLV81_09530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOM10375.1"
                     /translation="MRSNQEFYTYLQYSLYEAIAFFNFRIYIFGSEKTIKAIMFDSYC
                     EKEVLKSIINSAHKGLTPVIKEAIHFFKQYERKIFIQPPQCDLSLYTSNEQKVLKELL
                     LIQPGNTISYGELAAQCGFNGGARFVGNVMASNIFPVLFPCHRVIRSNGDTGNYSGGI
                     KIKEFLLAWEKENENSSVKQRTIHHAEYTNKK"
     gene            complement(5327..8350)
                     /locus_tag="PLV81_09535"
     CDS             complement(5327..8350)
                     /locus_tag="PLV81_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOM10376.1"
                     /translation="MKLIHHFVTHTITTLMLFCAIMATGFFAFTSLPSSLLPDAGNHD
                     ITIVVRYPGQSPYKIEEIIVNPLEDAISTIGGIEEIVSSSNDTEARIYITFEKDANLQ
                     FKVYEIHEKIEPVRAMFPDDVNEPEIYLQGNDYSPIMVIGLSSTQHSLNKLRDIAEGI
                     YKKNIERIEGVSQIEIGGGNKREVHVTVDNNYCVAHNISLQRITEQIQKNNVITSIGI
                     LHDTMKDYTLHLKSRFFTLDEIKHLPLLSGPGGLLTHLGQIAHVTDYAGIQDSLSRYN
                     TLDQVSLYIYKTSTANPLDVSQHIISILSQCSIPGVTAHITYNSAEEIQRALHNLYMS
                     CIAGIVLTMLVVYIFLKNIQATISVIIAIPFSFMAIAMYLFFSGVTLNVITLSAIAIA
                     TGMVVDNSIIVIEKIFSHPEDKIFSDEHIIASTQQVARALVASSLTTVVIFIPVLLLR
                     TKSTALFIQLAGTVIVAIVASLIVAVIFVPWCLRMTALHPLPSSHISIIQRLNLHIPR
                     ITLVAMFLGTCKKYVSITYVNSTMEYFFNNQKKLYGIILLALISTVIPISNITVEDIS
                     FTDQNKLFARLEMPSGSSLQATSHVALYIEKQLQHITSINNISTRIQQEHADFIIDTD
                     GTIDSEMIKRHLQLPLDTSLIFTSTGGTIRNNIEVIIKGSDVPVIRQLAYRFAEQMQA
                     TKAVEDIIYHFKDERPEVLIQFDRMKCAYSGIPIAYAGDYIRNLFYGPVITKYIDDRE
                     VDVRIKDNQYTVNEINDIVLPMDNKAVPLKNIATVTSSHIVTTVWHHDKMRSETISII
                     PKIPLYKMETILHNTIQQLSLPDGYYIEYDKTFALHKQSIIYMVIYICIAIVSVYMVI
                     ASIFESFMLPWIIIVSIPIAWIWAVWPLYIFNIQCNISTIMGFVVLTGIVVNNSILLI
                     DSYVQTIKSSRKKKLLLIKDYKTINQHRSKPMLITTITTVSGLLPLVISSGGSHLWQG
                     FALTVITGLCGSFACILIITPILFNTYQKNLNTQQ"
     gene            complement(8347..11418)
                     /locus_tag="PLV81_09540"
     CDS             complement(8347..11418)
                     /locus_tag="PLV81_09540"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOM10377.1"
                     /translation="MGTLIVQRPVTIIMAFSALCLFGIISACNLTMDLLPPVQYPEIS
                     IITLYPGASPSEIEQLITKPVEEAVNSVAGVTRIHSESIEGASLVVATLQWGSNVDFA
                     LLKVREKVDLVKGILPQDVYKPIVTRFDPNSLPVMNIAITSQDMDLRQLRFETEKNIV
                     PLFERIDGVGNAKVTGGKVRQIEVLVNRDALQAYNTGIHEIIDSIQSSHYNYPAGTLI
                     TGNKELLIRTAGEFKSIGDIENVPVKKTQDGRLVFLKHVARVIDGFKEITSQSFINGH
                     PCVTLSIIKESGKNTVEVCRKTRNLVDNLQKKYRRRLSFDIISDQSQYIRSAISSVVS
                     SAIAGSFIAFFVLLLFTQRFYSALIITIAIPVSIIIAFFFMNIFSININMMSLGGLTI
                     CVGMLVDCGIVVIESITNEKNTSLPKAIYTIAPSLIASTLTSVVIFLPLLLIKGLGGA
                     LFAHLAITVTIALLASLFVALLLIPALHTIHSQVAHHSKKQQHSVAIVHSNVLFHKVD
                     VLLHTIEHRYLLLLDTLLLQSKYIYSITFGIIVLGICAAIIMGRSLMPDTTQSHCTIR
                     LEAPPGTPLQQTIDMLHYIDSLLERYPDIDKRLITAGYNPEDYTEYFGREKNTNIGQI
                     HVQLHNGADARDCITYLENNVKLPSDICIEYNASNTDFLQVLRAGSGNITINCTGDSL
                     EDISATADAFIRECTGQPWITQCSSEIKQGKPELTIQADTDKLASFGLTLQDLASYIH
                     TAVYGTHSGKYTESDYEVDIVTRFDHRFRDSIDDIGMIPIQAHGGAPCTLLHTIASLS
                     NSQGYSSIIRNNQQRCIPIHIVTNSISQENAIEQLQRLWEKQKKQNVSFVLSQEMIET
                     KESLQSLYTILVLSIVLIYMIIAAQFESLLYPLLIMSSVPVALCGSFIFLFFTGKSIN
                     IMSLMGAVVLIGTIVNNAILLLDTIIHNYRQNGNVFASIAKSCTQRLRPIIMTSLTTI
                     CGLIPLALGLQEGADVQSPLAIAIIGGMIAGTGFIMLCLPHFIRLAIKDRP"
     gene            complement(11427..12971)
                     /locus_tag="PLV81_09545"
     CDS             complement(11427..12971)
                     /locus_tag="PLV81_09545"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOM10378.1"
                     /translation="MKVSLTIIFLSLTIVSCNYFVDIDPPEVISITPANRSVVLEDRP
                     VICVEFSESMDRPKTEEAFSFSGEHQPKGHFRWENNKLFFDLIENCKDATVYTIIIRS
                     SAEDSHGNNLKEDYTSSFSTSIDLIKPEAVSIQPADGARVSDIFSPLIVQFSEAVRLD
                     SVKKGFIITPYVVGDIMLLNDNSTLVFTPYNNYIHGTIYTITLSTDIVDIAGNHLLKE
                     YKSIFICGTDFTLPTLNPESVEPPDYSVGCFAHYNGTEVRLQPFTTTEGIDKNADVLI
                     RFSKAMQRIDTNESISISPPCNTFFSWEDDHSLRIIPSSPLDLMQTYVLTISSQAKDI
                     AGNTLDKVYNFPFKIYSQYSQPIEVIPYGNNSKNIYQISWDSNGNADDSLSRLLENDD
                     IIESAPPYSCYKLINNTDVTVLIFRIQFTNTTNTLSTIASLDLPSAMQAISFSLIASQ
                     SSSTLIPKIWKIEVPTNHPDCVDIYMFNLTPQSYYRLSINHGINGIRDSVGNYMLQPF
                     TMYLTM"
     gene            complement(12999..14423)
                     /locus_tag="PLV81_09550"
     CDS             complement(12999..14423)
                     /locus_tag="PLV81_09550"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOM10379.1"
                     /translation="MKALLHFTCMVSLISCSVDITPPEIVSYSPLHNAIVQSSCPVSV
                     CFSKSMNKDKTQKAFVLLYGEYGDSSIKGNFAWSNNDTEMTFTPELPLSNGYYRLIIE
                     KTACDTHDNTLVTQHISNFTIGLDTIPPEIESLQPDDCSENIALNTSITIAFSEPMDT
                     ASVEKNIRLTPSVDYTSMWDPANTILTLTPYKPLLFNTWYQIDIAKCEDLSHNMMIQP
                     VTCRFRTGSEYIRPVITGLYTTSISQNMAQTENFTIYEGANINDEIIITFNEPIDTSS
                     INRAFIISPQSTWDSYWGNLNQTCIIHFPQPLDVNTRYEIRINTNLKDVAQNTLLNDL
                     CVYIITNGNLSSIPSITKLECLIHEQKALTPLRVTDLGDESNENTTYEFTVHFSAPIL
                     RNSVPDNVTIQCLYGEQPEISGNINQFRWIDDSQTLKLTIAAIEGGNVYKLTFKGGTD
                     GIKSSNGIPMQKDVWYIFYFHPVD"
     gene            complement(14438..15820)
                     /locus_tag="PLV81_09555"
     CDS             complement(14438..15820)
                     /locus_tag="PLV81_09555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOM10380.1"
                     /translation="MDFFTLLRENKRKIYLLVLLSVIISTAYAILSLSLDNDTIIQEM
                     PIQVETFTIKAKAITKKVNSIATVQYKDKAIISSKIFGRVENIFVEQGKRVKKGQLLA
                     KIETYALELQLKEATAELKKAQANLRLAEEKLLQAKRNVEQRLKAIAKSRLELTDKYV
                     TLQNTEDILRKKEKLFKAGGVTETELNTLRTHYHTVKTQFLQAKKDYETLIVGFRDSD
                     IVKAGFTKPQDESKTNELFTLINTAMEQAECDAAKSQVEQAKTQIDIIKTNINEASIK
                     SPIDGIVAGRSIDIGEKVSEDTNLFVIINTSKVYCISQLNEIDLISIQPGQKVEITID
                     ALHKTYSGKVEIISPVIDQTSRTADVKIICSNIDNSLKPGMFARVSINVQTIPDAICI
                     PATSLKESDSSSVFIIKNTMAFEKKVTIKERMDEEVLVEGLQEGDIIANSQIKLLFDK
                     AKVKPILKTN"
     gene            complement(15848..17203)
                     /locus_tag="PLV81_09560"
     CDS             complement(15848..17203)
                     /locus_tag="PLV81_09560"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOM10381.1"
                     /translation="MKKLFCIFAVYIIATCSAWSQETLTLDNACELALKNSVKLERLK
                     AKIQSSRMIVHERWRELLPNVSFQYSKSDTVAIREDDTRFQSLACTVQYDIFSNQQSL
                     IAYSIAQIESMLANEEYTIEKNNIILSTKQAYFALQKKRKSIEIYQLLLKSLQLQKNI
                     ITAQQQLGMATELELIQVDGKIAEAQYNILSAQNQYANSLQDFATTLGINYASIQLPE
                     QEYTELQNITMASKENLISLSMQNRNELKKSYYTLIKTQKEYQLAQYYYLPRIQLFAS
                     YGYSGTEFPMNKKMWNVGLSVTSLLFGNTASLSNSYGKKDNGNTTDIKSSASVAVYDN
                     PSFIRSIIDAEAAYTEAQKYHQQLIQIIRTDVSKAYDTVMESLKKVEIARQTVLLLEK
                     QSSIENEQARLGDITRYDVLKTLVELSQARLRLQETITDVFVSMAALENAVGLPIESI
                     F"
     gene            complement(17200..17664)
                     /locus_tag="PLV81_09565"
     CDS             complement(17200..17664)
                     /locus_tag="PLV81_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10382.1"
                     /translation="MKKLMIMVSALVFFTACKSLDIQTVQHAPFANDSIFIKDVRIHN
                     ELYSTIKDSLVSHIVFHLQKYNLKAASYFNPIYREQDFKYTATVDIFITKTGDIFNQV
                     ENASLFIFINDASMHIASIRIISQSCRLQESFDQALIAKAIAKEIDAMVVKK"
     gene            complement(17667..18251)
                     /locus_tag="PLV81_09570"
     CDS             complement(17667..18251)
                     /locus_tag="PLV81_09570"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM10383.1"
                     /translation="MRTFTSIIVLLFYIACSNTNHAKELYNKALTRYANKDFEGASTL
                     CLESIKKADLPDSYLLLAKIYFFTNNSNFEKTIEKYIRLTDSSQGYILYARWFIRNQK
                     QDQAIRYLEKALLHSPNDPVALYLLGTIQYANKQYDDAIITFHRAFANYFPLMQIHRQ
                     LQEIYNEIHLPERAAKHQAILEAIKQFDDSTNGE"
     gene            complement(18251..19030)
                     /locus_tag="PLV81_09575"
     CDS             complement(18251..19030)
                     /locus_tag="PLV81_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10384.1"
                     /translation="MIHISFKMLIITTFITIIMFCISCTTATIPPKPQANSITEVYAI
                     DNYNKLHTVERMSPPSGFIIPDTITVTYVNNDDNDSSDKKELILWNNEIQKALLQQGI
                     QIINENRTITLFDDTKQQNQHPSLIIDTLRIEKISETLSVSHKHSDITAIYPYHYYFA
                     EINASISVHNVIVWRGSVRLTSFDLFQQKKLLTRPEVYFTLTSNYSYSNKMKQWISTN
                     PTISLSDNFSKYYDSSIDSELHKQQLIALAVTTLLSNIKGE"
     gene            complement(19324..20454)
                     /gene="tgt"
                     /locus_tag="PLV81_09580"
     CDS             complement(19324..20454)
                     /gene="tgt"
                     /locus_tag="PLV81_09580"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOM10385.1"
                     /translation="MHFEIIKKDRDTQARRGILHTTHGIVHTPVFMPVATRGAIRALT
                     IRDIEEIGFEIILSNTYHMYIRPGIELLKDVGGLHNFMNYHKPILTDSGGFQVFSLAD
                     LRKVHDNGVEFKSHIDGSKHFFSPQKVLDIQKVIGSDIMMVLDECVEYPSTHHAAQKA
                     VDRTLHWAATSFEYWKNTFDTDRQALFAIVQGSTFPDLRKHCVEALLEYDFSGYAIGG
                     LSVGEPKSEYAQITALTVPMLPWDKPRYMMGVGSPLEILEAIKHGVDMFDCVMPTRIA
                     RNGTLFTSKGRVNIKSGIYAQDFEPLDEHCQCYVCRTFTKAYLRHIYKMNEIAALIYN
                     TYHNLYFMKQFIEGIQTSIAEDRFLQEYRRWEEVYSNNTLKE"
     gene            complement(20475..21368)
                     /locus_tag="PLV81_09585"
     CDS             complement(20475..21368)
                     /locus_tag="PLV81_09585"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HOM10386.1"
                     /translation="MDTSTIKTVLIIGSGTMGQQIGFLCAMHGYRVHLYDVKEDALKS
                     AQERIVKLAEWFEKHTVKNASGALPLIHYFTDASKAAQDVDFVSESVPEDPALKAKVF
                     SEFNALCPQHTIFTTNTSSLVPSQIAKETGRPDKFLAFHFHDVRTTKIVDVMPHPQTS
                     KEALEVTIAFAKKLGQIPIVLKKENHGYVFNTMLMTLISSALTLAANEVADIYDIDRS
                     FMGVMGTPIGPFGIMDSIGLDTVYKVTKYWADLLNDKQGKKNAAFLQNYIDNGLLGTK
                     TGKGFYTYPHPEFSKPEFLQL"
     gene            complement(21529..22149)
                     /locus_tag="PLV81_09590"
     CDS             complement(21529..22149)
                     /locus_tag="PLV81_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10387.1"
                     /translation="MKNIILIFSFITFCIFLIVGCSSDSRNILGDTIAGKVTDAAIDT
                     VTTKSTEQTSTTKKSKQASADYVPSDADAHFIQPADYFISQNDFPGQGYIWVNLAKVI
                     TPPSAKTKNEGQFMIVESGKELWTKYFWKSRIASKSEIKIGKLIIAFDSNKTDDVYCA
                     PENKEDARTGSWFMAKITDTTDTYKGYVMVSGGYKVSLDNIRIPFK"
     gene            complement(22170..22646)
                     /locus_tag="PLV81_09595"
     CDS             complement(22170..22646)
                     /locus_tag="PLV81_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10388.1"
                     /translation="MYRKFFSIAIVVCFSMPLVLLALDDAHFIQDDDYFVSNQPYKQG
                     WIYVDLAKMIQPAKPETKNEAQFMTVREGKELWTKYFFQSRIAAIGECKVGTIVICFN
                     DNEKDEVYHAPEDKDSARNGSWFMARITDMSDLYKGYVTVSGGYKVNKENIRIILK"
BASE COUNT         6868 a         3889 c         4376 g         7607 t
ORIGIN      
        1 ctggttccgt gtattgcttt tatttcatgt tcatacaata tggtgacatt agtgaatttt
       61 gatagttcat caataagcac tttatcagca gttaatgcag gtaacagttg aaccactgtt
      121 actgatgttg caagttttgc cagatctatt gcagcttcaa tggctgagtt tccaccacca
      181 acaacaataa catctttgtc cttgaagagt ggagcatcgc agatagcaca gtatgcaaca
      241 cctttaccgg taagttcaga ttcacctggg acattgagtc ttcgccatcg cttaccggta
      301 gttataataa ttgattttgc ctgatagata ttcccatctt cacattctac ttttttaata
      361 tttccatcaa taactttttt tgccttgtat ccttgaagga atcctatctc aaattgttcc
      421 acctgttctt tgaaacgctg taccagtgag attccttcaa tgtgcctata gcccatataa
      481 ttttctactg ttgatgtttc ggcaacctgg ccaccaacat ccagtgtaat aagaccagtt
      541 ttaactcctt tgcgcataca atagactgca gcagtcaatg ctgctggtcc accaccaact
      601 atcaacacgt catatagtgt tgaactatcc agcggaacag ttcgctgctc aattgtttca
      661 agtcccggaa ggttaaatgt taattccatg gatactcctt ttaactattc ttatcttgaa
      721 atagtaataa aaaatttatt aatcgtcaag gaataaatta agtattctat tctcttataa
      781 tatgaattgt tcaatggagt ttgaaaatat gatataatta ttgagtgctt caaagtaaaa
      841 gcagcttata taaaaaatga atgaaaatgg gcacattaac gtgtgcccat ttatcgtggt
      901 agatatttta ctttaatatt tttttcaatg tgcgctctaa atagtctatg tgttcaacaa
      961 cagcttcacg tgccatatct ggtttatggg agtttatggc atccagaatc ttgatatggt
     1021 gttcataaat atcttttgat gtgtttttta ctttggccaa cttttcacgg ctataaggaa
     1081 ggacacccct caggatagtt gatattgcat tgagtatgtg cgcaaaaatg gtattgttgg
     1141 ttgcttcgta aaggtgatta taaaattgtg cgtaaaattt accaccttcg cgcttttgaa
     1201 tcaggttttc tactcccatc aattcaatct gagttctgaa tgagttaagt tttcttcttt
     1261 gctcctcatt tgcacgtaag gctgctaatg cagcggcttc agcgtcaata atgcggcgaa
     1321 cttccaaaag ctcccatatt tttttatggt caatctgaat taaaccttcg attggagttt
     1381 ttattgcatc atcaacaact gactttacat atttaccttt tcggccctga gactcaatat
     1441 atcccttagc ttctaaaagc ttcattgctt ctcgcaatga aatacggctg ataccaaaac
     1501 gccgtgtcat ttccagctca gatggaagcc tgtcacctgg ttttagaaca ccggaagaaa
     1561 tgagttctat aatttgtgaa accaccatgc ttgggatatc aggagctttt tcaatcttat
     1621 tgatcattaa ctgtatgcct ttatttcgtg ctgccatgta ttagatctcc tttttttatt
     1681 gatgtgttgt ttttaattat tgagaataac tatttaatct gattgtattt agtaatatcg
     1741 taatacataa tataatatta aattaattat gtcaacaaca ttaatattgc aaattggtat
     1801 tggaatatat acaaaataat ataattaaaa gaactggaaa attattgtat tattatatca
     1861 ttactataca aacaatctaa aaaaaatatg atttgattta ttgtaagtca atgttttgaa
     1921 tttttgtaat attgctataa tacaggtggt attgtaaggg tagataatta tttatatgaa
     1981 ttactgatta aaacaatggt ataattaaaa agtatatatc cctctgttac tttgatagtt
     2041 gtgtatggta atttgcatta cagtaaatgt atatgttgcg tataaacgaa agctacatgc
     2101 tatggtatgt acagtaaact acaatggtat aaaaagtttt aattgacgag tatttttaaa
     2161 taccatgtag tgaaaaaaga tattaatttg accggtatgt ggagggtgct gataaaccat
     2221 gaagataatt attactggcc caaaaggggt gggaaaatca acaattggga agagaatagc
     2281 tgaaatatta catatacctt tctttgaaac tgatgagatg attgaaactc tttttgaaga
     2341 aaaacatggc aataaaaaaa gttgcaggca gattgcaact gatcatggtg aatcattttt
     2401 cagagagctt gaaaaagagg caattaaaaa agctgcgttg tatgactggt gtataatagc
     2461 tactggtggc ggagcaatga tattcccgga aaacagaata cagcttagaa aagaaagtgt
     2521 aatgatttta ttgaaagcta cagttgattt tttatggcaa aggatgcagg ttaccggaat
     2581 cccgccattt ttacagggag acaatgcatt tgataagtat agccaacgtg ttcaaaaaat
     2641 atatgaagcc acggaacata taagtgatat tatttatact gtaacaaaag aaaatgaaga
     2701 tactgctcat gaggatttac tataccagat atcttttgta ttatcacaat gtatgcatgg
     2761 aaccaatacc tttggacagg ttctgaaggt aacaacattt ggtgaaagtc atgggaaagc
     2821 attgggggct gttgtcgacg ggttgaaacc cgggatccca ttatctgttg aagacattca
     2881 gaaacagctt gatcggcgta aaccaggtca aagttctgtt agcacccagc ggaaagaagc
     2941 tgacagtgtg gaaattgttt ctggtttgtt tgaaggtaaa acaacaggga caccgctgtg
     3001 tatgattgtg tataataaag atcaggattc cagggcttat gaatctatta aagatatatt
     3061 caggccaggt catgcagatt ttacattctg gcagaaattc ggtatacgtg attatcgagg
     3121 aggaggaagg tcatccgggc gtgagacagt gggtagagtt gctgcgggag ctatcgccct
     3181 gaaaatgttg aaagaaaaag gtgtaacaat agtggcgtat gctgaggaaa ttgctggcat
     3241 aaaaggagaa aaagtagata ttgattttat agaaaaaaat cctgtgcgct ctgccgatcc
     3301 ggataaagct caggcaatgg aacaggcaat taaacaggct caggctgaac atgattctgt
     3361 tggcggtata gtagcatgta ttattaaagg attgcctgca ggtttgggcg atccggtatt
     3421 ttgtaagctg gatgcgcggc ttgcgatggc cgttatgtca attggtgcag taaagggtat
     3481 tgagtttgga agcgggtttg ccgctgcacg taagagggga agtgaaaata atgaccagat
     3541 gagtgatgga aaatttttga ctaataatgc aggtggtatt ctaggaggta tttcaacagg
     3601 gcaagatatt gtgatgcgta ttgcagttaa gccgaccccc tctatagcaa agccacaaaa
     3661 aactatggat atagacggaa ataccgttga tatttcaatt aaaggaaggc atgatccctg
     3721 tattgtgcca cgaattattc cagttgttga ggcaatggtt gctctggtgg tatatgatgc
     3781 gtggttgctt caggagagga tagggcgtta tgacggtact gtataactta tctgagcgtg
     3841 atgaagtttt taaaaatcct gtaataacca taggaagctt tgatggagtc cataggggac
     3901 acagggcaat atttgatcgt atgctggaat tagcacaatc attacatggg gaaccggtag
     3961 tttttacatt cagtgaacat ccgcgaaaac ttttaacacc acacacacca cctaagatat
     4021 tgacgactgc tgctgaaaaa atccatgcaa ttgaacaggt tggcattaac aatattgtca
     4081 tgatgccctt tacccatgag attgcagccc ttacagcaga ggaattttta tttaagattg
     4141 tattttacca tatagggaaa gcacatattg tggtaggata tgaccatgcg tttgggaaag
     4201 gaaggcaggg agattttgaa tttttaaagg gtttgtcatt acgtgaaggg tttttggttg
     4261 agcgtgttga gcctgtttgt attcagtcgc gtccggtttc ttcatcatgg atacgcactg
     4321 agattgagga tggaaatatc gcactggcaa atatgctttt gggaaggccc tataccttgc
     4381 gtggtactgt ggttaaaggg caaatgcgtg gccggctttt ggggtaccct acagccaaca
     4441 ttacccccga tgaccctgat aagataattc caaaggatgg agtgtatgca gtcagggtac
     4501 acataaatgg tatgctgtat catggtatgc ttaatattgg taccaatcca acatttgaaa
     4561 acactgaacg aacaattgaa gttcatatat ttgattttga tgcagatata tatggccaaa
     4621 ctctcgaagt gttttttatt gaacgcattc gcgacgaaaa gcggttcggt tctgttactg
     4681 aacttaagga gcaactgcat aatgataaac tggccagtga aaaaatatta gctgggaact
     4741 gaccatgcgc agtaatcagg aattctatac atatttacaa tattcactgt atgaagcaat
     4801 tgcatttttt aatttcagga tttatatttt tggtagtgaa aaaacaatta aagcaattat
     4861 gttcgatagt tactgtgaaa aagaggtatt gaaaagcata atcaattcag ctcacaaagg
     4921 tttaactccc gtaatcaaag aagcaataca tttttttaag cagtatgaac gaaagatatt
     4981 tatacaacct ccacaatgcg atttatccct ctatacaagt aatgaacaga aagttttaaa
     5041 ggaattactt ttgatacaac ctggcaatac tatatcgtat ggtgaacttg cagcacaatg
     5101 cggttttaat gggggtgcac ggtttgtggg aaatgtaatg gcaagcaata tatttcctgt
     5161 tttgtttccc tgccatagag taatacgaag taatggtgat acaggcaatt acagtggtgg
     5221 tataaagata aaggagtttt tacttgcgtg ggaaaaagaa aatgagaatt ccagcgttaa
     5281 acaaagaaca atacatcacg cagagtatac gaataaaaaa taaatcttac tgttgagtat
     5341 ttaaattttt ttgataagta ttaaataaaa ttggtgtaat aatcaatata catgcaaatg
     5401 aaccacacag tcccgttatt actgttaaag caaaaccctg ccatagatga cttccaccag
     5461 aagagataac cagcggcaac aaaccagata cagtggttat tgttgttatc agcattggtt
     5521 tgcttcgatg ctgatttatg gttttataat ctttgatcag taataatttc ttttttcttg
     5581 aagatttgat cgtttgaaca taactatcta tcaaaagtat tgaattgtta accacaatac
     5641 cggttaatac tacaaacccc ataatggttg atatattaca ctggatatta aagatatata
     5701 atggccagac tgcccatatc catgctattg gtatggatac gatgattatc catggtagca
     5761 tgaacgattc aaatatagaa gcaattacca tgtacactga gacaatagct atgcatatat
     5821 agatcaccat atagatgatg gattgcttat gcagtgcaaa ggttttgtca tattctatgt
     5881 aatatccatc ggggagggat aattgttgaa tggtgttgtg gagaattgtt tccattttat
     5941 acagaggaat ctttggaata atgctgattg tttcagatcg catcttatca tgatgccata
     6001 cagtggtaac aatatgtgat gaggtaactg ttgcaatatt ctttaatggg acagccttat
     6061 tatccatagg taatactata tcattaattt cattaacagt atactggtta tcttttatgc
     6121 gtacatcaac ttccctgtca tcaatgtatt tggttatcac aggaccatag aaaagatttc
     6181 ttatgtaatc gcctgcatag gcaatgggaa tcccggagta tgcgcatttc attctgtcaa
     6241 attgaatcaa cacttcggga cgctcatctt tgaaatgata gataatatct tcaactgctt
     6301 ttgttgcctg catttgttca gcaaatctat atgctaactg gcgaataact ggcacatcac
     6361 ttccttttat gataacttca atgttattgc gaattgtgcc acctgtgctg gtaaaaatta
     6421 atgaagtatc caatggaagc tgtaaatgcc gttttatcat ttcactatct attgtgccat
     6481 ctgtatcaat aataaaatct gcatgttcct gttgaattcg cgttgagatg ttgttaatgg
     6541 atgtaatatg ctgcaattgc ttttctatat aaagggcaac atgggaagtt gcctgtaatg
     6601 atgaacctga gggcatttcc aatcgtgcaa agagtttatt ttgatcagta aagctaatat
     6661 cttcaacagt aatgtttgag attgggatta cggtgctgat aagtgctagc aggattatgc
     6721 catagagttt cttttgatta ttgaaaaaat attccatagt tgagttaaca taggtgatag
     6781 atacatattt tttgcatgta ccaagaaaca ttgcaactaa tgtaatacgc ggaatatgca
     6841 gatttaatcg ttgtattata gatatatggg atgatggtag tggatgaagt gctgtcatgc
     6901 gtaaacacca gggtacaaat ataacagcca ctatcagtga tgcaactata gcaactatca
     6961 cagtgccagc taactgtatg aataacgcag tactctttgt tcttaatagc agcacaggaa
     7021 taaatatcac tactgtggtt aaactggatg caaccagagc tctggctacc tgttgcgttg
     7081 atgctataat gtgttcatcg gaaaagattt tatcctctgg gtgtgaaaat attttttcaa
     7141 tgacaatgat gctattatca acaaccatac ctgttgcaat ggcaattgca gaaagtgtta
     7201 tgacgttaag tgttactcca ctaaaaaata aatacattgc tatggccata aatgaaaatg
     7261 ggattgcaat aataaccgag atagtagcct gtatattttt taaaaatata taaaccacca
     7321 gcattgtaag tactatacca gctatacagc tcatataaag gttgtgaagt gctctttgta
     7381 tttcttctgc actattataa gttatatgtg cagtaactcc aggtatagag cattgtgata
     7441 gtatgctgat aatgtgctgt gatacatcta agggattagc tgtacttgtt ttatagatat
     7501 ataatgaaac ctgatccaga gtgttgtatc gtgatagtga atcctgtata cccgcatagt
     7561 ctgttacatg cgctatttga cctaaatgcg ttagtaaacc tccaggtcct gataatagtg
     7621 gcaggtgttt aatttcatcc agtgtaaaga atctgctttt aaggtgcaat gtataatctt
     7681 tcattgtatc atgcagaatc ccgatggaag taataacatt gtttttttgg atttgttctg
     7741 ttatgcgttg caatgatatg ttgtgagcaa cacagtaatt attatcaaca gttacatgga
     7801 cctcacgctt attgcctccg cctatctcaa tctgagaaac accctcaatg cgttcaatat
     7861 tttttttata tatgccttct gcaatatcac gcagcttgtt aaggctatgc tgtgtacttg
     7921 ataaccctat gaccattata ggtgaatagt cattcccctg aagatatatc tcaggttcat
     7981 taacatcgtc tggaaacatt gcacgcacag gttctatctt ttcatgtatt tcatacactt
     8041 taaattgtag attggcatcc ttttcaaagg ttatgtatat gcgtgcttca gtatcgttgc
     8101 ttgaagaaac aatctcttct attccgccta tagtgcttat tgcatcttca agtgggttga
     8161 caattatttc ctcaatttta tagggggatt gcccaggata ccgtaccaca atggttatgt
     8221 catgattacc tgcatcgggc aaaagggatg aaggcaatga tgtgaatgca aaaaagcctg
     8281 ttgccatgat tgcacaaaaa agcatcaacg ttgttattgt atgagttaca aaatggtgta
     8341 ttaatttcat ggcctgtcct taattgctaa acgtatgaaa tggggcaggc agagcatgat
     8401 aaagcctgtt cctgcaatca tgccgccaat gatagcaata gcaagtggtg actgaacatc
     8461 agcgccttcc tgtaatccta atgccagtgg tataagccca caaatggtag taagtgatgt
     8521 cattatgatt ggacgcagtc gctgtgtgca ggattttgca attgatgcaa atacattacc
     8581 attttggcga tagttatgaa taatagtatc aagaagtaat atagcattat tcacaatagt
     8641 acctataaga acgactgccc ccatgagaga catgatattt attgattttc ctgtgaaaaa
     8701 aagaaatatg aatgatccac ataaagcaac aggtactgat gacatgatta acagtggata
     8761 gagaagtgat tcaaattgtg cggcgataat catgtatatt aacacaatag aaagtacaag
     8821 tatagtatac agcgattgaa gtgattcctt tgtttctatc atttcctgtg ataaaacaaa
     8881 cgaaacattt tgttttttct gcttttccca cagccgttgt aattgttcta ttgcgttttc
     8941 ctgtgaaatg ctgtttgtta caatatgaat gggaatgcat cgctgctgat tgtttcgtat
     9001 aattgacgaa tatccctgtg aattggaaag tgaagcaatt gtatgaagta atgtacatgg
     9061 agcaccacca tgagcctgaa tgggtatcat tcctatgtca tctatactgt cacggaacct
     9121 gtgatcaaat cgagtaacga tatcaacttc ataatctgat tcagtgtatt ttcccgagtg
     9181 tgttccatac acagcagtgt gtatatatga agcaagatcc tgaagtgtta atccaaaaga
     9241 tgctaacttg tcagtatcag cctgtatggt aagttcaggc ttgccctgtt taatctcact
     9301 ggaacattgt gtgatccatg gttgtccagt acattcacgt ataaatgcat cggcagtggc
     9361 actaatatct tctaaagaat cgcctgtaca attaatcgtt atattaccac tacctgcacg
     9421 taacacctgt aaaaaatcag tatttgatgc attatattca atgcaaatat cagatgggag
     9481 tttaacattg ttctctaaat atgtaatgca atccctggca tctgctccat tgtgcagttg
     9541 tacatgaatc tggccaatat tagtattctt ttccctgcca aaatattcgg tgtaatcttc
     9601 gggattatag cctgctgtta tcaatcgttt gtcaatatca ggatagcgtt ccaacaatga
     9661 atcaatatag tgaagcatat caattgtttg ctgcaatggg gtaccaggtg gtgcttccaa
     9721 tctgatggta caatgactct gtgttgtatc aggcatcagt gacctgccca taataatagc
     9781 tgcacatatt ccaagcacaa taataccgaa agttattgag taaatatatt tactctgtaa
     9841 aagtagtgta tcaagcagca aaaggtatct gtgttcaata gtgtgcagca atacatctac
     9901 tttgtggaaa agtacattac tatgaactat cgccacacta tgctgttgct ttttagaatg
     9961 atgagctact tgtgaatgga tggtgtgcag tgcagggata agaagcaatg caacaaaaag
    10021 agaagctaaa agggcgatag ttactgtaat ggctaaatgc gcaaataggg cgccgcccaa
    10081 tccctttatg agtagtagtg gcagaaaaat gaccacactg gttaatgtag atgcaataag
    10141 tgatggtgcg atggtatata tggctttcgg gagtgatgta tttttttcat tggtaatgga
    10201 ttcaattact actatgccac agtcaacaag catgcccacg catatggtca atccgcctaa
    10261 tgacatcatg ttgatattaa tggaaaatat gttcatgaaa aagaaggcaa tgataattga
    10321 tacaggtatt gctatggtaa taatgagtgc tgaatagaaa cgctgtgtga aaaggagaag
    10381 aacaaaaaat gcaatgaaag aaccagctat agcagatgac acaacactgg atatggcact
    10441 gcgtatgtat tgcgactgat cgctgattat atcaaatgat agtcttctgc ggtatttttt
    10501 ttgcaggttg tcaacaaggt tgcgcgtctt gcggcacacc tcaacagtgt ttttgcctga
    10561 ctccttaata atggataggg taacacaggg atgaccattg ataaatgact ggctggtgat
    10621 ttctttgaag ccatcgataa cgcgggcaac atgttttaaa aagactaagc ggccatcctg
    10681 ggttttctta acaggaacat tttcaatgtc accaatgctt ttaaactcac ctgcagttcg
    10741 tataaggagt tctttatttc ctgtaatgag tgtacctgca ggataattgt agtgagatga
    10801 ctgtatgcta tcgattatct catgtattcc agtattgtat gcctgcagtg catcacggtt
    10861 tacaagtact tcaatctggc gtactttgcc tcctgttact ttagcattgc caactccatc
    10921 aatacgttca aaaagtggta ctatattttt ttctgtttca aagcgcagct gtcgtaaatc
    10981 catatcctgt gatgtaatag ctatgttcat aactggtaaa gaattaggat caaagcgtgt
    11041 aacaattggc ttatagacat cctgagggag gatacctttt acaagatcaa ctttttcgcg
    11101 aacctttaaa agagcaaaat caacattgct tccccattgt aatgtggcaa cgaccaggct
    11161 tgcgccttca atggattcag aatgtatacg cgttacacca gcgacgctgt taactgcttc
    11221 ttcaactggt tttgtaataa gctgttcaat ttccgatggg gaagcaccag gatatagtgt
    11281 aataatggaa atctcaggat attgtacagg aggcagcaga tccatggtga ggttacacgc
    11341 tgatataatg ccaaaaaggc acagtgcgct aaatgccatg ataattgtta cagggcgttg
    11401 tactattaat gttcccatat agtgcattac atagtaagat acatggtaaa cggttgtagc
    11461 atgtaattgc ccacgctatc ccttattcca ttgatgccat gattaatgga taatctgtaa
    11521 tatgattgtg gagtaagatt aaacatatat atatcaacac aatcgggatg attagttggt
    11581 acttctatct tccatatttt tgggataagt gttgaacttg attgtgatgc aatcaggcta
    11641 aatgatatag cttgcattgc tgagggtaaa tccagtgatg caatagtgga cagtgtattt
    11701 gttgtattgg taaattggat cctgaatatc aagactgtaa catcagtatt gtttatgagt
    11761 ttgtaacatg aatatggtgg tgccgattcg atgatatcat cattttctaa tagacgtgac
    11821 aaactatcat cagcattccc attggaatcc caacttatct gatatatatt tttgctatta
    11881 ttaccatatg gaataacttc aattggttgt gaatattgtg aatatatttt aaatgggaaa
    11941 ttgtatacct tatcaagagt attgcctgct atgtctttgg cctgtgaaga aatagtcaga
    12001 acatacgttt gcatcaaatc taatggtgaa gagggtatta tacgaagact gtgatcatct
    12061 tcccatgaaa aaaaggtatt acagggaggg gatatgctga tactttcatt agtatcaata
    12121 cgctgcatag cctttgaaaa tcgtataagt acatctgcat ttttatcgat gccttctgtt
    12181 gttgtgaaag gctgcagtcg aacttctgtg ccgttataat gagcaaaaca gccaacagaa
    12241 taatcaggtg gctccactga ttcaggattt agagttggaa gggtaaagtc agtaccacat
    12301 ataaagatac ttttatattc ttttaataaa tgatttcctg ctatatctac aatatctgtt
    12361 gatagagtaa tggtgtatat tgtgccatga atatagttat tatacggtgt aaatactaga
    12421 gtagaattat cattcagcag cataatatca ccaacaacgt atggggtaat tataaagcct
    12481 ttttttactg aatctaacct gactgcttca gaaaattgta caatcagtgg agaaaatatg
    12541 tcagatactc gtgctccatc cgcaggttgt attgatacag cttcaggttt gattaggtct
    12601 atgcttgttg aaaatgacga tgtgtaatct tctttaaggt tgttgccatg actatcttcc
    12661 gcactggagc gaataataat agtatataca gttgcgtctt tacagttttc aataaggtca
    12721 aaaaaaagtt tattgttttc ccatcggaaa tgcccctttg gctgatgttc tccgctaaaa
    12781 gaaaaagctt cttcggtttt tggtctatcc attgattcag aaaattctac gcaaataaca
    12841 ggtctatctt ctaaaactac ggacctgtta gcaggggtta tgctaatgac ttctgggggg
    12901 tcaatatcca caaaataatt acaggataca attgtaagag aaaggaaaat tatggtaaga
    12961 gatactttca tagtaaactc ctatacatga aacatttctt aatccactgg atgaaaataa
    13021 aatatatacc atacatcttt ctgcataggt atgccattgc ttgactttat gccgtcagtg
    13081 ccacctttaa atgttaattt gtatacattt ccgccttcaa ttgcagcgat agttaatttc
    13141 aatgtctggc tatcatctat ccacctgaat tgattaatat tgccacttat ttcaggttgt
    13201 tcaccataca ggcattggat tgttacatta tctggtacag aattcctgag aataggtgct
    13261 gaaaaatgta ctgtaaactc ataggtggtg ttttcattcg attcatcacc taagtcagta
    13321 acacgtaaag gagttagagc tttttgttca tggatcaagc attcaagttt agtaattgat
    13381 ggtatactgg aaagattacc atttgttata atatatacac atagatcatt gagcaatgtg
    13441 ttttgagcta catctttgag atttgtatta atacgtattt catatctggt gttaacatct
    13501 aatggttgcg ggaaatgaat aatacaggtt tgatttaaat ttccccagta tgaatcccag
    13561 gtggactgtg gtgaaataat aaatgctcta tttatcgatg aagtatctat tggttcatta
    13621 aaagtaataa taatttcatc gtttatattt gctccttcat atattgtaaa gttttcagtt
    13681 tgcgccatat tttgtgaaat tgaagttgtg tagagtcctg taataactgg acgtatatat
    13741 tcactacctg ttctgaacct gcatgttaca ggttgtatca tcatgttatg ggaaagatcc
    13801 tcgcattttg ctatatcaat ttgataccag gtattaaaca gtaacggttt gtaaggagtt
    13861 agagttagta tggtgtttgc aggatcccac atgcttgtat aatcaaccga aggtgttaat
    13921 cgtatatttt tttcaactga agcggtgtcc atgggttcgg aaaaagcaat tgttatggat
    13981 gtattcaatg ctatattttc actacaatca tctggttgca aagattctat ctctggtggg
    14041 attgtatcca gtcctattgt aaaatttgat atatgctggg taaccagagt attgtcatga
    14101 gtatcacatg cggttttttc aataataagg cgatagtaac cattgctaag tggcaattca
    14161 ggtgtaaatg tcatttcagt gtcattgttg ctccaggcaa aatttccttt tatggaacta
    14221 tcgccatatt caccatataa caatacaaat gccttttgtg ttttatcctt attcattgac
    14281 ttagaaaagc aaacgcttac aggacatgat gattgcacaa tagcattatg cagtggtgaa
    14341 tatgaaacaa tttctggtgg tgttatgtcc acgctacatg aaatcaaaga aaccatacat
    14401 gtgaaatgta ataacgcttt catagatacc tttaccatta attggttttt aatattggtt
    14461 ttactttagc tttatcaaat aaaagcttta tctgtgagtt tgctatgatg tcgccttcct
    14521 gtagcccttc aaccaatact tcctcatcca ttctttcttt aatggtgact ttcttttcaa
    14581 aggccatagt attttttata ataaagacac tggaactatc gctttcttta agagatgttg
    14641 cagggatgca aatagcatct ggtattgttt gtacattgat agatacacgg gcaaacatgc
    14701 caggtttcag gctattatcg atatttgaac atataatttt cacatctgct gttcgtgatg
    14761 tctggtcaat tacaggactt atgatttcaa ccttgccaga atatgtctta tgtaatgcat
    14821 cgatggtgat ttcaactttc tggccaggtt gtatagaaat caggtcaatt tcattaagct
    14881 gagagatgca atataccttg gatgtgttaa tgatgacaaa aagattggta tcctcactta
    14941 ccttttcgcc tatatcaatt gaacgtccag caacaatgcc atcaataggt gatttgattg
    15001 aagcttcgtt tatattggtt ttgattatat ctatctgagt ttttgcctgt tcaacctggc
    15061 tttttgctgc atcacattct gcttgttcca ttgcggtatt tattaatgta aaaagctcat
    15121 tcgtttttga ttcatcttgc ggtttggtaa aaccagcctt gactatatca ctatctctaa
    15181 aacctacaat cagtgtttca taatctttct tagcctgtaa aaattgtgtc ttgaccgtat
    15241 gataatgagt ccgcaatgtg ttgagttctg tttcggtaac accacctgct ttaaataatt
    15301 tctctttttt tctgagtata tcttctgtat tttgaagcgt tacatattta tcagtaagtt
    15361 ccagtcgtga ttttgctatt gctttcaggc gttgttcaac atttcttttg gcctgtaata
    15421 atttctcttc agctaagcgt aaatttgcct gagctttttt gagctcggct gtagcttctt
    15481 taagttgcaa ttccaatgca tatgtttcaa tctttgctaa taattgtcct tttttaactc
    15541 gcttgccctg ttctacaaat atattttcta cgcggccaaa tatcttggat gaaataatag
    15601 ctttgtcttt atactgaact gttgcaatgc tgtttacttt ttttgttatt gcttttgctt
    15661 ttattgtgaa ggtttcaacc tgtatgggca tttcctgaat tattgtatcg ttatccaaag
    15721 ataacgaaag tatggcatat gctgttgaga taattacgga cagtaaaact aataaataaa
    15781 tttttctttt attttctctg agtaacgtaa aaaagtccat aatcaattat acatctccct
    15841 catattttta aaatatggat tcaataggca gtccaacggc attttctaat gctgccattg
    15901 atacaaagac atcagttatt gtttcctgca gcctgagacg tgcctgcgaa agttcaacca
    15961 gtgtttttaa cacatcatag cgggtgatat cacctaaacg agcctgctca ttttcaattg
    16021 aagattgttt ttcaagaagc agtacggttt gcctggctat ttcaactttt ttcaaagact
    16081 ccatcacagt atcataagct tttgaaacat cagtacgtat tatctgaatg agttgctgat
    16141 gatacttctg cgcttcagtg tatgctgctt cagcatcaat tatagatcgt ataaacgatg
    16201 gattatcata gacagcaaca gatgcactgg actttatgtc tgtagtatta ccattgtctt
    16261 tttttccata tgaattgctc agggaagctg tgtttccaaa aagtaaactt gttactgaca
    16321 gaccaacatt ccacattttt ttattcatgg gaaattctgt accgctataa ccgtaacttg
    16381 caaaaagttg aattcgtggt agatagtaat actgtgccag ctggtattct ttttgagttt
    16441 ttataagtgt ataataactt ttcttaagtt cattgcgatt ttgcattgat aaggatatca
    16501 ggttttcttt acttgccata gtgatgtttt gtagctcagt atattcctgc tctggcaatt
    16561 gtatagaagc atagttaata cctaaagtgg tagcaaaatc ctgcaatgaa ttggcatatt
    16621 gattttgtgc gctgagaata ttgtactgcg cttcagcaat tttcccatca acctgtatca
    16681 gctcaagctc tgtggccata cctaattgtt gttgtgcagt aatgatgttt ttttgtaact
    16741 ggaggctttt taaaagaagc tgatagattt caattgattt tctctttttt tgtagagcaa
    16801 agtatgcctg tttagttgaa agaatgatat tatttttttc tatggtatat tcttcatttg
    16861 caagcataga ttcaatctgt gctattgaat atgcaattaa cgattgttgg tttgaaaaaa
    16921 tatcatattg tactgtacat gcaagcgatt gaaaacgtgt atcatcttct ctaattgcaa
    16981 cagtatcgct ttttgaatat tgaaaagaaa cattaggaag caactcacgc catcgttcgt
    17041 gtacaatcat acgtgatgat tgaatctttg cttttagcct ttcaagcttt acactgtttt
    17101 tcaatgctaa ctcgcatgca ttgtccaatg tcagtgtttc ctgactccat gcagaacagg
    17161 ttgcaatgat gtaaactgca aaaatacaaa acaacttttt cattttttaa caaccatagc
    17221 gtcaatttct ttggcgatag ctttggctat cagtgcctgg tcaaaacttt cctgtaagcg
    17281 acagctttgt gaaataatcc gtatgctagc aatatgcatt gatgcatcgt tgatgaatat
    17341 aaaaagtgaa gcattttcca cctgattgaa aatatcgcca gttttagtga tgaaaatatc
    17401 aacagtagct gtatatttga aatcctgttc gcgatatatg ggattaaaat atgaagcagc
    17461 tttgagatta tacttttgca gatgaaaaac aatatgagaa actaatgaat cttttatggt
    17521 gctatatagc tcattgtgaa tgcgcacatc tttgataaaa attgaatcat tagcaaatgg
    17581 agcatgctga actgtttgaa tatctaaaga cttgcatgcg gtaaaaaata caagggcaga
    17641 taccataatc ataagctttt tcatagttac tctccatttg ttgaatcatc aaattgcttt
    17701 atggcttcaa ggattgcctg gtgctttgca gcacgttcag gcagatgtat ctcgttatat
    17761 atttcctgta attgtctgtg tatttgcatg agcgggaaat agttggcaaa agctctgtga
    17821 aatgttataa tagcatcatc atactgttta tttgcatact gaatagtgcc cagaagatat
    17881 aatgcaacag gatcattggg agaatgcaga agtgcttttt caagatatct gattgcctga
    17941 tcttgtttct ggtttcttat aaaccatcgt gcatatagaa tatatccctg agaactatcg
    18001 gttaaccgga tatacttttc tattgttttt tcaaaattgc tattattggt aaaaaagtat
    18061 atctttgcta aaagcaaata tgaatcaggt aaatctgctt tttttattga ttccaggcat
    18121 aaagtacttg caccttcaaa atctttgttt gcataacgtg taagtgcttt gttatacaat
    18181 tcttttgcat gattggtatt gctacaagca atgtagaaca aaagtactat tatactagta
    18241 aatgttctca ttattcacct ttgatatttg atagtagtgt tgttacagcc aatgctataa
    18301 gctgttgttt gtgcagttcg ctgtctatac tggaatcata gtatttactg aaattgtcag
    18361 acaacgatat ggtaggattg gtgcttatcc attgtttcat cttatttgaa taagaataat
    18421 tgcttgttag cgtaaagtaa acttcaggtc tggttaacag cttcttttgt tgaaataagt
    18481 caaatgatgt taaacgaaca ctaccacgcc acacaatcac attatgaaca gaaatacttg
    18541 cattaatttc agcaaaatag taatgatagg gatagattgc tgtaatatcc gaatgtttat
    18601 gcgataccga tagagtttca gatattttct caatacggag tgtgtcgatt attaatgatg
    18661 gatgttggtt ctgctgttta gtatcatcaa acagagtaat cgttctattt tcattgatta
    18721 tttgaattcc ctgctgtaat aatgctttct gtatttcatt attccataga atgagttcct
    18781 ttttatcact tgaatcatta tcatcattgt tcacatatgt aacagtgata gtatcgggaa
    18841 taatgaaccc agatggtggg ctcatgcgtt ctacggtgtg aagtttattg taattatcga
    18901 tagcatatac ctctgttata ctgttagcct gtggttttgg tgggatagtt gctgttgtac
    18961 atgaaataca aaacataatt attgttataa aagttgtaat aattaacatt ttaaatgaaa
    19021 tgtgtatcat gatgtaattc ccctgggtaa aagtgttttt cttatctcaa tagctccatt
    19081 gctatggttt tctctgtgtg gatcattacg tttcttcaca tattgagtgc ctggtgtttt
    19141 aatttcagat ttctctgcac cttgttatcc agaatggaat aaaagacaca gcgttttaga
    19201 ctcactatat acaacgcagg aattgaaaaa aattaacggg gaaatataaa aaatttttat
    19261 ttttttaaaa tttttttatg gttaatacgg tttatagtgt tgttaaaggc aaagagttat
    19321 attttattcc ttaagagtat tattgctgta aacctcttcc catcttctat attcttgcag
    19381 aaatctatcc tctgcaatac ttgtctgtat accctcaata aattgcttca taaaatacag
    19441 attatgatag gtattgtaaa tgagggctgc tatctcattc atcttataaa tatgacgcag
    19501 atatgcttta gtaaatgtcc ggcatacata acactggcaa tgttcatcca gaggctcgaa
    19561 atcctgtgca tatatacccg atttgatatt gacgcgccct tttgatgtaa agagtgtccc
    19621 attgcgggca atgcgtgttg gcatcacgca atcaaacatg tccacgccat gttttattgc
    19681 ttcaagtatt tcaagtggac tcccaacacc catcatgtag cggggtttat cccatggcaa
    19741 catgggtact gttaaggctg ttatctgagc atattcactt ttaggttcac caacggaaag
    19801 accgccaatg gcatagcctg aaaaatcata ctccagtaat gcttctacac aatgttttcg
    19861 taaatcaggg aaggtgctgc cctgtactat tgcaaagagt gcctgtctgt ctgtatcaaa
    19921 ggtatttttc cagtactcaa atgatgtggc tgcccagtgc agagtacggt ccactgcctt
    19981 ttgtgcagca tggtgtgttg aagggtactc aacacattca tctaaaacca tcatgatgtc
    20041 agagccaata accttctgta tgtccagtac cttttgcggg ctgaaaaaat gtttggaacc
    20101 atcaatatgt gatttgaact caaccccgtt atcgtgtacc ttgcgtaaat cggccaatga
    20161 aaatacctga aatccgcctg aatcggttaa tataggtttg tgatagttca taaaattatg
    20221 aagaccaccc acatctttga gcagttcaat accaggacga atatacatgt ggtatgtatt
    20281 ggacagaatt atctcaaaac caatttcttc aatatcgcgt attgttagtg cgcggatagc
    20341 acctcgagtt gcaacgggca taaagacagg tgtgtgcaca ataccatggg tagtgtgaag
    20401 aattcccctg cgtgcctgtg tgtcacgatc ttttttaata atctcaaaat gcattagtat
    20461 agcctgtatg attgttataa ctgcaaaaat tctggctttg aaaattctgg atgcggatac
    20521 gtataaaatc ctttgccagt ttttgttccc agtaacccat tgtcaatgta gttctgtaga
    20581 aaagcagcat tctttttacc ctgtttgtca tttaacaaat ctgcccagta ttttgttact
    20641 ttgtatactg tatccagccc aatactgtcc attatgccaa atggaccaat aggtgtgccc
    20701 ataacaccca taaaacttct atctatatca tagatatcgg ctacctcatt ggctgcaagt
    20761 gtcagtgctg aacttattaa cgtcatgagc attgtattga atacataccc atgattttct
    20821 ttttttaaca caattggaat ctgccctaat ttttttgcaa aagcgatagt tacctctaaa
    20881 gcttctttgc tagtttgtgg atgtggcata acatcaacaa tctttgttgt tctgacatca
    20941 tgaaaatgga atgccaggaa tttatcaggc ctgcctgttt cttttgcaat ttgtgatggt
    21001 accagtgacg aagtattggt agtaaatata gtgtgttgtg ggcatagagc attaaactca
    21061 ctgaatactt ttgcctttaa tgctggatct tcaggtactg attcgcttac aaaatcaaca
    21121 tcttgcgccg cttttgacgc atcggtaaaa taatgtatca atggtaatgc accagatgca
    21181 ttttttacag tatgtttttc aaaccattcg gccagtttta caattctttc ctgagcactt
    21241 ttaagagcat cttcctttac atcatacagg tgcactctat aaccatgcat agcgcataaa
    21301 aagccaatct gctgccccat agttccggaa ccaataatta acaccgtttt aatggtactg
    21361 gtatccatac tatcctccat agattattat atagtaacta tcgtatgtga tttatgtgaa
    21421 atactgtgtt aatggcaaat cggggtgcat gttcgtcaag tataattata atcaaaaaaa
    21481 ggagcataaa gccccctttt ttaattaata atgaaagcag atataatatt atttaaatgg
    21541 aatacgaatg ttatcaagtg aaactttgta accacctgaa accattacgt accctttgta
    21601 tgtgtcggta gtatcagtaa ttttagccat gaaccacgat ccggttctgg cgtcttcttt
    21661 gttttcgggg gcacaataca catcatctgt tttattgctg tcaaaagcaa taataagttt
    21721 gccaatttta atttcagatt tggatgcaat tcgtgatttc cagaaatact ttgtccataa
    21781 ttctttgccc gattccacaa tcataaattg accttcattt tttgttttag cactgggagg
    21841 tgttatcact tttgcaagat taacccatat atagccctgt ccggggaaat cgttctgcga
    21901 aataaagtaa tcagcaggct gaataaaatg tgcatcagca tcagatggta cgtaatctgc
    21961 cgaagcctgc tttgattttt ttgttgttga tgtttgctca gtagatttgg ttgttactgt
    22021 atcaattgca gcatcagtaa ctttgcctgc aatcgtatca cccaggatgt ttctactgtc
    22081 actgctgcag ccaactatca aaaatataca aaaagttata aaagagaata ttaaaataat
    22141 atttttcata tgacattcct cctttggaac tattttaaaa ttattcttat gttttctttg
    22201 ttaactttat aaccgcctga aacggttaca tagcctttat acaggtcaga catatcggtg
    22261 atcctggcca tgaaccatga gccgtttcgt gcactatcct tatcttcagg agcatggtac
    22321 acttcatctt tctcattgtc attgaaacaa attacaatag tgccaacctt acactcacct
    22381 atagctgcta tccttgattg aaagaaatat tttgtccaca gctctttgcc ttccctaacc
    22441 gtcataaact gggcttcatt ttttgtttca ggttttgccg gttgaatcat ctttgcaaga
    22501 tccacataga tccacccctg tttgtatggc tgatttgaaa caaaataatc atcatcctgg
    22561 ataaaatgag catcatcaag agcaagtaaa accaacggca tgctgaaaca tacaacgatc
    22621 gcaatactga aaaatttgcg atacatatac aacctccttt gattataatt ttgcacttta
    22681 taacaagttg gcatgtgtta atgtcaaggt gtaataaagt gtttacattc tttatccaat
//
LOCUS       DAORYL010000048        22354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1963_length_22354_cov_12.793264, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000048 DAORYL010000000
VERSION     DAORYL010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 22354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22354
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1963_length_22354_cov_12.793264"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            370..1584
                     /gene="tyrS"
                     /locus_tag="PLV81_09600"
     CDS             370..1584
                     /gene="tyrS"
                     /locus_tag="PLV81_09600"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOM10389.1"
                     /translation="MIKNDIELLKRGVEEIIPLDEFEKKIERSQKENKPLIIKAGFDP
                     TAPDIHLGHTVLLRKMRHFQQMGHTVIFLIGDFTAMIGDPSGKSQTRKRLTREEVLSN
                     AETYKKQVFKILDPEKTIVDFNSRWCMPMSFADVLDLASRYNVARMLERDDFAKRYKD
                     GVPISILEFLYPLIQGYDSVALKADIELGGTDQKFNLLVGRDLQREYGQEPQVIMTLP
                     LLVGLDGVQKMSKSLNNYIGIDEDPQQIFGKVMSISDDLMFLYYELVTDVPKAEIESY
                     KKGIQDGSVHPKDVKVRLAREICAQFYNEEIAQKAKMEFDKIFVQKDLPSEMPEFKVP
                     ESEKKNNTIWIVKLLVLAGMAKTNGEARRLIEGGGVYIDGNKVENPDAEIPCSASFII
                     KVGKRRFARILP"
     gene            1636..2199
                     /locus_tag="PLV81_09605"
     CDS             1636..2199
                     /locus_tag="PLV81_09605"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOM10390.1"
                     /translation="MSEYSDNELIDRVIAGDTDAFADIITRYQNKIFRYVYTRVYDYD
                     EACDMTQDIFLITMESLKTFRKESQFSTWLFSIAVNYCKNHRRKNRHKVYSIHSAQDE
                     TEIQIPDIRQNQEELVITNESLQIMLEEIHKLPDDYRDILVLRDIEGESYSAIAQALH
                     LSLANVKVRIHRGREMLKTRLQQRGLL"
     gene            2196..2843
                     /locus_tag="PLV81_09610"
     CDS             2196..2843
                     /locus_tag="PLV81_09610"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOM10391.1"
                     /translation="MNHIPVHKLSEYIDGILDAQDNDAITSHVSECPECRQNLLQLQK
                     MIALLSSLKTISIPATFATDTLARAKKQYYHKRNYRSLRGSVFLAAMMLIAIITMLPD
                     SIKHDQFKNHITQNTQNNQRLDCIIPTNMDLNTAMMVIQQHNARVLDFSNSYVIVESD
                     VSSFSSIRQIINNYESYRPNPLSNVGITYMQQQEKTYPELTHANKKKYIFRLQLR"
     gene            complement(2855..3382)
                     /locus_tag="PLV81_09615"
     CDS             complement(2855..3382)
                     /locus_tag="PLV81_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10392.1"
                     /translation="MLKQRKHYLIDRSIQVTIATKILVIPIILLTCFSLILLFFVYQN
                     SNYISKISANQENLIEMFMTTPALMDKENQIVVAGEKTFRENLGILQSIKNNNLILLY
                     CAIGITIFQAVLLFVYGVYLTHRIAGPVYVMRQYLKEIHQGKTPQFRQLRKGDLLTDF
                     YDDFVKAIKTLQTKK"
     gene            3645..5789
                     /locus_tag="PLV81_09620"
     CDS             3645..5789
                     /locus_tag="PLV81_09620"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOM10393.1"
                     /translation="MITLSLIGSLLSSFMLLFTIFYGLYKNYHDRILRYYSYFGLCAF
                     GILFTMFLTYAFPNELDLTLVNKITQASTVLTFSALFVLSLIFPKTEKQVPFLVITII
                     LVPAYIIAYIAVFTDMNITAAYFKDGKLVREFRFFYTVYLSVAFGYVLLGVINFIRKY
                     ITTKVELYRKQMRFVFIGTGIAMTFAAVFSIIMPRFFGYVDLYVLGPSLATFIGVSSL
                     FYAVISYQMMDIRTAIHKTTMYTVISTAIFLPMFALVYIYDINILGLKTIPLYIIAFI
                     IVVMFMVLSKYIQPIIDKAFKRKHYELQEYLDTFVKEISTIRDIDELIKKTVSTIHNT
                     FYLKSTYFLLYNNEVRSYQLEYSMGEAPVSPSINRLSPIVRWFNRNQRIVTLDNVYID
                     ETSFIDVRDEFIQYFTTNNIIIMIPIYHRRVLTGILCLGEKDTLAAYKPDEIELLSFL
                     SNETNINLSHAITYQEAKKEQMLQRTITISSELLAKAVPRALPNIAGIKYGAFYIPRQ
                     GGGIDYFDFIRPGLQGIGTIATDIAGIGMQSALYSVIMRSAFHTSLSDAHSSYSVIQK
                     LNRVIHNYSQGKGILITAYYYYIDVRNMRLIYTNAGFPPMELYRVEKSDFSSLDTEGI
                     PLGYDPTFNYGMGRTNILRGDIGILYSKALITSKNSRGEQFTLDHVRNIIKENRMKMP
                     SDIATIIQDEFKAFMGISSPESDIVVIIFKSY"
     gene            complement(5793..6728)
                     /locus_tag="PLV81_09625"
     CDS             complement(5793..6728)
                     /locus_tag="PLV81_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015153278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOM10394.1"
                     /translation="MKICITGGAGFIGSHIAEECVKKGYEVIVFDNFSTGKKENLSFV
                     KNFPGSSLKIVKGDVADYKAVSKAVKGCNAIFHEAAVASVQKSIENPPATFESNAKGT
                     LNVLEASRNNGVTKVIFACSAAIFGDHPEIPKREDSPVLPKSPYGADKYISEVYLRLY
                     NELYNLTTVSLRYFNVFGPRQDPSSPYSGVISIFTTRIAQGKDITVFGDGKQYRDFIF
                     VKDVVQANMLALEKIHSGFHYYNVGYGKATDLNMLISILQKIYQKEITVNYTDARPGD
                     IKESVTDPSKIIKELGFTPGYSVEEGLRILVESLQ"
     gene            7349..8791
                     /gene="purF"
                     /locus_tag="PLV81_09630"
     CDS             7349..8791
                     /gene="purF"
                     /locus_tag="PLV81_09630"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOM10395.1"
                     /translation="MKTEYEYLDGKPREECGIFGIYNHPKAANLTYLGLYNLQHRGQE
                     SSGIASSDGYHIYRYAGMGKVVDVFTEEHINNLKGNIAIGHNRYSTTGSSFLRNAQPL
                     RAHSVLGPIVLAHNGNLVNYAMLRRALEKEGAIFQTSIDSEVIVHLMARSGLTTFLDA
                     LIYALKAIKGAYSLLVMNYNTMYAIRDPYGFRPLVLGKLGDSIVIASETCAFDIIDAE
                     YIREIEPGEMLEISPTGIKSYFPFASVKNSLCVFEYIYFSRPDSIIFGQSVHEMRLRM
                     GAMLAKQAPVDADIVVPIPDSSVCAALGYARASGIPYEMGMIRSHYIGRTFIEPEQRI
                     RDFGAKIKFNIVRSAVEGKRIVVVDDSIMRGTTMRKIIKMFRKGGAKEIHVRISAPPT
                     RFPCFYGIDIPTRSELIASSHTIEEIQKYLRVDSLAYMTVESMLKALDVHHGKFCTAC
                     FDGNYPVEFQEADDYQKCLFDDTVACGEYY"
     gene            8837..8909
                     /locus_tag="PLV81_09635"
     tRNA            8837..8909
                     /locus_tag="PLV81_09635"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8870..8872,aa:Trp,seq:cca)
     gene            8922..9620
                     /locus_tag="PLV81_09640"
     CDS             8922..9620
                     /locus_tag="PLV81_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10396.1"
                     /translation="MNPHITTIHFDATTFSIDDRLVHLLQAKTHYNELSPNKIHDTFL
                     NHTFFHGGDNSDTSQVQQTNLSKGLLHMLSQTRQKAQPEVFYCKVETHHYNPLQTCIV
                     DEYPMYPFMLTLGQQFEHWCHEHALVSEQYYAHLAGMWMLQHCVESLAQILAMDRYCT
                     VYPGSSKDVPLQVNRQLYMVFSKEARSSGITLNEQYMFWPLHTIAGIILPHTGENTCQ
                     SCTLLHCQFRNILT"
     gene            9676..>10796
                     /gene="alr"
                     /locus_tag="PLV81_09645"
     CDS             9676..>10796
                     /gene="alr"
                     /locus_tag="PLV81_09645"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOM10397.1"
                     /translation="MILTHPAYCNRPTRAEISIPNLLNNFSIVRALVAPHVKIMAMVK
                     ANAYGHGIVRVSQELIKAGVDYLGVAYLEEALFLRQHGISTPILVCGAINTEQIPEFI
                     LNDVAITSSSLDKSKAIHNAAKALGKKAKVHLKIDTGMERIGVHWYNAENFINETMSL
                     DGIEVTGIFSHLAKAESDADFTKQQIQRFATIIEYMHKHSICPPYVHLANSAAIINHP
                     ESHFTMVRPGIMLYGYNPNGYNPDITFNGKKLLPVMTLKTKVSYFKVVPAHTGISYNH
                     LYTTSKQTRIVTLPVGYGDGYSRLLSNKGEVIIRGKTYPVVGAVCMDQIMVDIGPDGT
                     AYNGDDVLLFGQMDGYSIPLESLCQKIGTITYEILTNISP"
     assembly_gap    10797..10896
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10897..11690)
                     /locus_tag="PLV81_09650"
     CDS             complement(<10897..11690)
                     /locus_tag="PLV81_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM10398.1"
                     /translation="MKQTILLVDDDVNVLEGYKRQLRDEYEVTTFSSASEALDHIQQG
                     NEFSVIISDYRMPVMDGVVFLEKVKELSPDSVRVMLSGQADLQAVIDVINKGNIFRFL
                     TKPCEPDDLKKAIADCVRQYELITAEKELLGKTLAGSIKVLTDLLALSKPIAFERSRR
                     LKAIVKIITHNLAIQKGWQLDISTMLSQIGCVTIPDDILKKAFRGLQLGDDEIAMYKN
                     HPIIACDMLKKIPRLEEIAQIIKYQDKYYDGSGYPEDSIAGEQIPEG"
     gene            complement(11719..12078)
                     /locus_tag="PLV81_09655"
     CDS             complement(11719..12078)
                     /locus_tag="PLV81_09655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM10399.1"
                     /translation="MKIILFVDDDINLLNSLKRILHKKKNEWDMHFISSATEALAFIQ
                     EHKYDCVVVDYKMPQVNGIELLKAAAQHNPQAKRVLLSGQVDEDVFAKSRSITHAYLS
                     KPCEPEELMKVLEELLQ"
     gene            complement(12103..14091)
                     /locus_tag="PLV81_09660"
     CDS             complement(12103..14091)
                     /locus_tag="PLV81_09660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM10400.1"
                     /translation="MENNNHISYETIQQILNLVNTGLIIIDEDGIVIVTNEYFTHTVC
                     NTHIKSFHNINISSIQEIPQEIKRLILLDNTELLESKKANQYSTTITVQGNRQFSFRI
                     IKTWFEEKNKKYILTLFVDISQEVAIYKELKKSERKYRYLLDYVPLGLLTLDASTLKI
                     IDTNRKALELLRLSEYTHLIGKYLPDVLNVKESNIYELIQSKWESIENYECKLQYGEE
                     ERYLRMNFAAITLDDTNSYLVTLDDVTPLKKAQEELTISHGQVKHLLSAIDSILIGVS
                     RNDIITHWNRTAEKIFGINSSDAVGKNIIGFNIPWEWNKIYMGITASIVEKRPITLPD
                     VRYDSLFGQKRLLGITINPIMDEDGILLGYLIFGRDITDKRISESEMVQAQKLQSIGQ
                     LAAGIAHEINTPAQYVNDNLHFIKDVVLDLEALLNTCDELTSVLFAAKQLPQRLQKLL
                     NEINTIKEKIDYTYCKEELPKAVEQSIEGISKIVSIVKSLKNFAHPDIQKKVIINLNK
                     TLEDVITITRNEWKYISDLTTDFKHIDIMIPCFPNQLNQVFLNMIINARDAIQEAIDK
                     KLIEKGNIEISTDMNTDYAIVAIKDNGIGISEEIKSKIFDPFFTTKEVGKGSGQGLAI
                     AHSIIYDKHKGMIRVESEYGKGTTFYVYLPLRDLSLEL"
     gene            14390..15202
                     /locus_tag="PLV81_09665"
     CDS             14390..15202
                     /locus_tag="PLV81_09665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOM10401.1"
                     /translation="MIKDAMYYDSYKEDRDLILEYNQTSLADVNLVNIIGIGIDNCTR
                     QQAVVKVLRMVEEGGVHHVMALNPYKLHRLTTNNDLNLIASKADLKLAVGAGLPWAAK
                     FLGRPLKERIHFMSFLMDLIRIAEIKELTIFMVGAKPEIIEQAYANIKKSFPKIRIVG
                     RHGGYFNKERAANVIEAMRKSEAQIILIGLGFPKEDKWIYEIKNQFKNTVFIGIGGSI
                     DIISGQKRKAPAYFMEKGLDWFYRIITRPWRYLRLLRLLLFYIQVLWQRLRK"
     gene            complement(15240..15455)
                     /locus_tag="PLV81_09670"
     CDS             complement(15240..15455)
                     /locus_tag="PLV81_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10402.1"
                     /translation="MEYVSVILCRNCGSRYVEVNEWTQDKKAVFHCRTCGKKEIVEWF
                     TLGRCQVTQTELQKARDTKAKPGKYER"
     gene            complement(15470..16717)
                     /locus_tag="PLV81_09675"
     CDS             complement(15470..16717)
                     /locus_tag="PLV81_09675"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOM10403.1"
                     /translation="MNEKKYNLEYLERQLHNLSKLVEINRIINSTLDMAKLFTIIMEI
                     IKEIMETEASTLFLYDEKANELVFKVALGEAGDKLQEKYRVKMGQGIAGWVARERKGV
                     YVNDVYSDDRFDPNFDKQTGFTTRSIICVPLLYKGKLLGVIQAINPINRPAFTDEDMH
                     LFLAFADQAVLAVQNAIFFEHAIEEARMRHELESARSIQHSLHPAISENYGLCKVAAR
                     YLPAREVGGEFYELLYQKNAVNIALCDVHNKGVPGALNASLVNGIVKGLMNVYENAVS
                     PVFINTLRVVKEQNLQNVSLFYGMINLHSRTLTFMNTGEAYPILVRDGIARYMRFSYT
                     RDRKKIVVKLKTNDMFIIVTDGIVNCKNRNARLFGLKQVMDTCCQYDEPSQCIDGLLT
                     KVKEFMQGLAQREDISVIAVKIQ"
     gene            17581..18078
                     /locus_tag="PLV81_09680"
     CDS             17581..18078
                     /locus_tag="PLV81_09680"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOM10404.1"
                     /translation="MTYLIDGYNLIYKFPHLEELMLQDNLTEARKELLDILKTFARLT
                     GKRIRVVFDGQKNVEIPISSEKVANIDVYYSLHYSADFLIKEFIRKDIQPRNTTVVTS
                     DKDIIDFVSRFKTRTMKSEDFADFINQTIEDYTVAQTPEKEENPTLSSEEIEYWEKLF
                     IKRKK"
     gene            complement(18080..18847)
                     /locus_tag="PLV81_09685"
     CDS             complement(18080..18847)
                     /locus_tag="PLV81_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOM10405.1"
                     /translation="MLTLGMDIGSITTKAVVLDNKSIIGSKIIFTGFNAAKAGEKVTQ
                     ELLAELGLSLDKIEKIVATGYGRKSVAFAHNNITEITCHAKGAHFLDNSIRSIIDIGG
                     QDSKVIVIDEQGKVKDFAMNDKCAAGTGRFLEVMARALEVDLDEFGELSLKANNPAKI
                     SSLCTVFAESEVISLIAKGETRDNIIAGIHQSIASRIMAMANRVGITNPVMMTGGVAK
                     NIGVVTAIEKLLDSPVKVSDNAQIIGALGAALLAAEN"
     gene            complement(18917..19429)
                     /locus_tag="PLV81_09690"
     CDS             complement(18917..19429)
                     /locus_tag="PLV81_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10406.1"
                     /translation="MKRFFTIALVVIIFACNGCAKKSIRTGPLICTVHGIHNCYNKIE
                     IAQCIGVSEKSIDDYYIFSESGSGDSSTNESKLKSLVCDKIVDEVMLRIQMIYSSKQF
                     QRWQFTDMSLPVIFRTFVKKENQQEMVVIGFVKKDDLSPKAIIYYLPLEYKMDIIGRE
                     KNNDKLQPEW"
     gene            complement(19506..20369)
                     /locus_tag="PLV81_09695"
     CDS             complement(19506..20369)
                     /locus_tag="PLV81_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOM10407.1"
                     /translation="MKRTFIVQVLLFIILTIHSVYAIELKPGSWVIVNVPALENSTKT
                     VPVKLYIPKEYSKNSRTIIALHDYDGSMNSWQNNTNIAYYANMYNMVIVCPNMPKTVY
                     ESQFFDETTVKWNDLPAALWIGTILIPYLKNTVGLKIDKNSLGVCGFSIGARGALRIG
                     EMYSDTVSAIACLSGYYDMLAHVKNNMFVAVYGKYNDNPQRWAAIDNAIDDAKNLKNV
                     AVFLAHGNKDSRVPMEQSFMLAVKLKQLQKEQKSGYNFTFVEKKYKMHDWNFCQSVLP
                     EMMAFFNENLK"
     gene            complement(20691..21365)
                     /gene="radC"
                     /locus_tag="PLV81_09700"
     CDS             complement(20691..21365)
                     /gene="radC"
                     /locus_tag="PLV81_09700"
                     /inference="COORDINATES: protein motif:HMM:NF000642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOM10408.1"
                     /translation="MQTKCELPVQRCINGESIDDLSDAELLAILLGTGVKGRDVMECA
                     FGLIQHFGGVSGIYHAGIREIAAINGMGIVKAVRVKAAMELGKRLIAPRNYEEAIDSP
                     RIVWECLVGDIACSRQEEFWVMILDSKNRLIKKSRVSIGTISEALVHPREVFRDAIRE
                     AASSIIIAHNHPSGVLKPSVNDIEITKRVAQAGAIVGIQLLDHVIVTDNDYLSLRETD
                     ETLFTQ"
     gene            21590..22150
                     /locus_tag="PLV81_09705"
     CDS             21590..22150
                     /locus_tag="PLV81_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10409.1"
                     /translation="MKVSIDAILQSAHQLKSTINVDEHKSGNKNKVTGDKVNIEHRVM
                     ARLDEIHNQIKDIQTTLSRYQVIDDGIRMILNDKKAGGTNAPNIIDATQYNNRPVLKE
                     FLGDSYDENTLTHKQDQLKNLVADSITGLQRLQVEFENIVASDLARNVQLKDKIDEIN
                     SLIPRNIKNIQHLHHLDAETVKRLIQ"
BASE COUNT         7038 a         4348 c         3710 g         7158 t
ORIGIN      
        1 aggtaaaaca actgaaggga tgggttttac cggaacaggt gaaggcatag ctgcaatggc
       61 tacatgcctt gtggtagtaa agaataatgt ataaaaatta agccctaccc ggttaatgat
      121 ttccaaaaaa tcacaaatac aggttgacag ccaacaacaa aattatattt atagtgcatt
      181 caaaccaaca agtattgaat ataagtttga caagaacatg cacggtcact gagtgtatta
      241 atcaggataa cagtttaccc ttaatatctc ttcttaaatg aggagtagta ctgactctct
      301 cctttcctta tccaggagat ggcttgggga tgtacataaa tcctattttc aagatacagg
      361 agcactgcta tgataaaaaa cgatattgaa ctgctaaaac gaggtgttga agaaattata
      421 cctctggatg aatttgaaaa aaaaattgaa cgatcacaaa aagaaaacaa acctctcatc
      481 ataaaggctg gctttgaccc aactgcacca gatattcact taggacatac agtgttgttg
      541 cgcaaaatgc gccatttcca gcaaatggga catacggtta ttttccttat tggcgatttt
      601 actgccatga ttggtgaccc ttcaggaaaa tcccaaactc gtaaaagact gacccgtgaa
      661 gaagtgttga gcaatgctga aacctacaaa aaacaggttt tcaaaatcct tgaccctgaa
      721 aaaacaatag ttgattttaa ttcacgctgg tgtatgccca tgagctttgc cgatgtttta
      781 gacctggctt cacgctataa tgttgcacga atgttggagc gtgatgattt tgccaaacgc
      841 tataaagatg gtgtacctat ttccatactg gaatttttat atcccttaat tcagggatac
      901 gattccgtgg cactgaaagc tgatattgaa ttaggcggta ccgatcagaa atttaacctg
      961 ctggttggaa gagacctgca gcgtgaatac ggacaggagc cacaggttat catgacgcta
     1021 ccgttactgg taggccttga tggtgtccag aaaatgagta aatcgcttaa taattatatt
     1081 ggaatagatg aagacccaca gcagattttt ggaaaggtta tgtcaatatc tgacgatctc
     1141 atgtttttat attatgaact cgtcactgat gttccaaaag ctgaaattga aagctacaaa
     1201 aaaggcatac aggatggctc agtgcatcca aaagacgtaa aggtaaggct ggccagggaa
     1261 atatgtgcac agttttataa tgaagagata gcccaaaaag caaagatgga atttgacaaa
     1321 atatttgtac agaaagacct gccatcagag atgccagaat ttaaagtacc cgaatctgag
     1381 aaaaagaaca atacaatatg gattgtgaag ttactggttt tggctggcat ggcaaaaaca
     1441 aatggtgaag cacgcaggct tattgaaggt ggtggtgtgt atattgatgg caacaaggta
     1501 gaaaaccctg atgccgagat tccctgcagt gcatctttta ttattaaagt tggaaaacgc
     1561 cgttttgcca gaatacttcc gtaacttttt tcccgtttct acactaattt tttcagtaac
     1621 ttatatctac taactatgtc agaatatagt gataatgaac ttatagatag ggtaattgca
     1681 ggagacactg atgcatttgc tgatatcatt acacgatacc agaataagat tttcaggtat
     1741 gtgtatacac gtgtgtatga ttatgatgag gcatgtgata tgacccagga tatattcctc
     1801 attaccatgg aatcgttaaa aacattccga aaagaatctc aattttctac atggcttttt
     1861 agcatcgctg ttaattactg taaaaatcac agaagaaaga acagacataa agtatattca
     1921 atacactctg cccaggatga aacagaaata cagattcccg atattcgcca gaaccaggaa
     1981 gaacttgtca taaccaatga gtcattacag atcatgcttg aggaaatcca taaattgccg
     2041 gatgattaca gggacattct tgttttacga gatatagaag gtgaatcgta ttcagctata
     2101 gcacaagcgt tacatttaag tttggccaat gttaaagtac gcatacaccg agggcgcgag
     2161 atgttaaaaa ctcgtttgca gcaaaggggg cttctatgaa tcatatacct gttcataagc
     2221 tttctgaata tatcgacggc attcttgatg cccaggataa cgatgctatt acatcacatg
     2281 tatcagaatg tcctgaatgc aggcaaaacc tccttcaact tcagaagatg attgctctac
     2341 tatcctccct gaaaacaata tctatacctg caacatttgc aactgatact ctggcacggg
     2401 ctaaaaaaca gtattatcat aaacggaatt accgttcact gcgtggttca gtttttctag
     2461 ccgctatgat gcttattgct ataataacca tgctgcccga ctcaatcaaa catgatcaat
     2521 ttaaaaatca cattacacag aatactcaaa acaatcaaag gttagattgt atcattccaa
     2581 ccaatatgga cctgaacacc gcaatgatgg ttatacagca gcataatgcc cgtgtacttg
     2641 atttttcaaa ctcgtatgtc attgtggaat ctgatgtgag cagtttttca tctatccggc
     2701 aaatcataaa caattatgag agctatcgcc ccaatccgtt atcaaatgtg ggaattacct
     2761 atatgcaaca acaggaaaaa acttacccgg aattaacaca tgccaacaaa aagaagtata
     2821 tttttcgttt gcagcttcgc taaaaatata ttttctactt tttggtttgt aatgtcttaa
     2881 tagcctttac aaagtcatca taaaaatcag ttaaaagatc gcctttgcgt aattgtctga
     2941 attgtggggt tttgccctga tgtatttctt ttaaatactg gcgcatgaca tacacaggac
     3001 ctgcaattct atgggtaaga tacacaccat aaacaaaaag caacactgcc tgaaatattg
     3061 ttatcccaat cgcacaatat aaaagaataa gattattgtt tttaattgat tgtagtatgc
     3121 ctaaattttc tctgaatgtt ttttcaccag ccactactat ctgattttcc ttatccataa
     3181 gggcgggtgt tgtcataaac atttctatga ggttttcctg attagctgaa atcttgctaa
     3241 tataattgct gttctgatac acaaaaaata atagaatgag cgaaaaacag gtcaacagta
     3301 taattggtat aaccagtatt tttgttgcga tagtaacctg gatggacctg tcgatgaggt
     3361 aatgtttgcg ttgcttcaac ataacttatt cctctacatt aattattttt tatatacccg
     3421 tgtaaaaatc atatgtagca tcttaattat ttttctgtaa ttatatagta taaattatat
     3481 tacgtcaact acgtttttag ttttactaat aaaacttgac aatcaggtaa ataaaatgct
     3541 attattagca acgaaggtac tattttgttc taacaacatc ggtaagagca agtttaaagc
     3601 agtacgtatc ccatgccaaa cttttaattg ggaggaatat ctcaatgatt accttgagtt
     3661 taattggttc attattgtca tcatttatgc tgttatttac tatattttat ggactatata
     3721 aaaactatca tgatagaata ttacggtatt actcatactt tggattgtgt gcatttggca
     3781 tactttttac catgttttta acgtatgcat tccccaatga acttgatcta acattagtaa
     3841 ataaaattac tcaggcatca acggtattaa ctttttcagc tttatttgtt ctatccttaa
     3901 tattcccaaa aaccgaaaaa caggtcccat ttctggttat tactattatt cttgttcctg
     3961 catacatcat tgcctacata gcagttttta ctgatatgaa tattactgca gcatatttta
     4021 aagacggaaa attagttcgt gaattcagat ttttttatac tgtatattta tcagtagcgt
     4081 ttggatatgt tcttttaggt gttattaatt ttatccgtaa atatataacc acaaaagttg
     4141 aactgtatcg caagcagatg cgctttgtgt ttattggtac tggtatagca atgacgtttg
     4201 ctgctgtttt ttccatcatt atgccacgat tctttggtta tgttgatctc tatgttttag
     4261 gaccatcact ggcaacattt attggagtat catcactttt ctatgcagtt atttcatacc
     4321 agatgatgga tatcaggacc gctattcata aaacaaccat gtatacagtc atttccacag
     4381 ccatattttt acccatgttt gcactggttt atatttatga tattaatata ctgggattaa
     4441 aaaccatacc tctctatatt atagcattta tcatagtcgt catgtttatg gttctttcaa
     4501 aatatattca acctataata gataaagctt ttaaacgcaa gcattatgaa ctgcaggaat
     4561 atctggatac atttgtcaaa gaaataagta caatacggga cattgatgaa cttataaaaa
     4621 aaacagtttc tactatacac aatacatttt atctaaaatc aacatatttc cttctgtaca
     4681 ataatgaagt ccggagctat cagcttgaat attcaatggg agaagcacct gtttcacctt
     4741 ctataaaccg cttatcacct atagtacgct ggttcaacag aaaccagagg atagtaactc
     4801 tggataacgt ttacatagat gaaacttcat ttattgatgt acgggatgaa tttattcaat
     4861 attttacaac caataatatt ataatcatga ttcctattta tcatcgtcgt gttttgactg
     4921 gtattctatg cttgggtgaa aaggatacac tggcagcata taaacctgat gagattgaac
     4981 tattatcttt cttatccaat gaaaccaata ttaacctttc tcacgcaata acatatcagg
     5041 aagcaaaaaa agaacagatg ttgcagcgca caattaccat ttcaagcgaa cttttagcaa
     5101 aagctgttcc cagagcattg ccaaatattg caggcataaa atatggtgct ttttatattc
     5161 cacggcaggg tggaggtatt gattactttg atttcattcg cccgggttta cagggaatag
     5221 gtactatcgc caccgacatt gccggcattg gtatgcagag tgcattatat tctgttatca
     5281 tgaggtctgc tttccacacc agcctcagtg atgctcattc atcatatagt gtcatacaaa
     5341 agcttaaccg cgttattcat aactattctc agggtaaagg tatattaatt acagcctatt
     5401 actattatat tgatgttcgc aatatgaggc ttatttacac aaatgctgga tttccaccaa
     5461 tggaattata ccgtgtagaa aaaagcgact tttcatcact ggatactgaa gggatcccac
     5521 tgggatatga tccaaccttc aattatggca tgggccgaac caacatactc aggggagata
     5581 ttggcattct gtattccaaa gcattgatta catctaagaa ttctcgtggt gaacaattta
     5641 cattagatca tgtacgaaat ataataaaag aaaatcgtat gaaaatgcct tccgacatcg
     5701 caaccataat acaggatgag ttcaaggcat ttatggggat aagttctcct gaatcggata
     5761 ttgtggtaat tatttttaag tcatattaaa tgttactgta acgactcaac aagtatccgc
     5821 aacccctctt ctactgaata gccaggtgta aatcccagtt ctttgattat ttttgaggga
     5881 tcagtcactg attctttaat atcccccggt cgcgcatcgg tataattaac cgtaatttct
     5941 ttctgatata ttttttgtag tattgatatc agcatattca aatctgtagc cttgccatat
     6001 ccaacattat aataatgaaa acctgaatgt attttttcca gtgccagcat atttgcctgc
     6061 acaacatcct ttacaaaaat aaaatccctg tattgtttcc catccccaaa tactgtgata
     6121 tctttaccct gggcaattct ggttgtaaaa attgaaataa cgccagaata cggggatgat
     6181 ggatcctgac ggggtccaaa tacattaaaa tagcgcaatg acaccgttgt aaggttatat
     6241 aactcattgt ataagcgaag ataaacctct gaaatatatt tgtcagcacc atagggtgat
     6301 tttggtaaaa ctggtgaatc ttcacgcttt ggtatttcag gatgatcccc aaagatagcc
     6361 gctgaacagg caaatatcac ttttgttact ccattattcc tgcttgcttc aagcacattc
     6421 aatgtccctt ttgcatttga ttcaaatgtt gcggggggat tttcaatgga tttttgtacc
     6481 gaggcaaccg cagcttcatg gaatatcgca ttacaacctt ttacggcttt agaaactgct
     6541 ttataatcag ccacatcacc ttttactatc ttcaagcttg aacctgggaa gttttttaca
     6601 aatgaaaggt tttctttttt accagtcgaa aaattatcaa agactatcac ttcataccct
     6661 tttttaacac attcttctgc aatgtgtgag ccaataaacc cagcaccacc ggtaatacag
     6721 attttcatta taatttcccc ttaaaaatct attttaacta taactctttt tgcactttat
     6781 ttgtccaaac tgcaacaaaa aaagtgctaa aagaaaaatc atattacttt attttcatgg
     6841 gaacatcctt aaaacttgtt tttagaagtt cccttacata gtatagattg tatcgaatct
     6901 tgttggaaaa ctgtaacgta actacactat tcagttatta tcaataccac aataaataac
     6961 taaattacag tatcaaaacc tcaatacaat atcaagcata tttattacat tgctgcgttt
     7021 taaaaagtca tttcgataaa aagttcacaa cgtccatatt agtaattttg acacacaaca
     7081 ttgcagaatt gtatcaatat gattgcattt tatcagcaaa tatgtgcaag atattgccta
     7141 tgaacaattt tggggaaccc cacataaaga gcatttgccc agttactatc gcccaaatag
     7201 caaattttta ttgattttat acaaaatacc attatattgg ttattggaat taactttatt
     7261 attcttaata tctatacaag cttcatttac aaaggatact actggtctaa atcgtaaaaa
     7321 tctaaccagg ctatttttca ggataattat gaaaacagaa tatgagtatt tggatggtaa
     7381 accgcgtgaa gaatgtggta tttttggcat ttataatcac ccaaaagcag caaatcttac
     7441 ctatttaggc ctttacaacc tgcaacatcg agggcaggaa tcaagcggca ttgcttcatc
     7501 cgatggatac catatctatc gttacgcagg aatgggaaaa gttgtcgatg tatttaccga
     7561 agagcatatc aataacctca aaggaaacat agccataggc cacaaccgct attcaactac
     7621 cggctcatca tttttacgca atgcacaacc tttgcgagca cactcagtac tgggccccat
     7681 agtgctggca cataatggca acctggtaaa ctacgcaatg ctaaggcgtg cccttgaaaa
     7741 agaaggagcc atttttcaaa catccattga tagtgaagtt atagtgcatt taatggcgcg
     7801 ctcaggtctt actacatttc ttgatgcact catttatgcg ctcaaagcaa taaaaggcgc
     7861 ctattcactg cttgtcatga attacaacac aatgtatgca atccgtgatc catacgggtt
     7921 tagaccgctg gtgctgggta aattgggtga ttcaattgtc atagcgtctg aaacctgtgc
     7981 ttttgatatc attgatgcag aatatattcg ggagatagaa cccggggaaa tgcttgaaat
     8041 atcacctacc ggcataaagt catacttccc atttgcaagt gttaaaaatt ctctgtgtgt
     8101 gtttgaatat atctattttt cacgacccga tagcatcatc tttggccaat cggtacatga
     8161 gatgcgtctt agaatggggg ctatgcttgc aaagcaagca cctgttgatg ctgatatcgt
     8221 tgttcccatt cctgattcat cggtgtgtgc agcgttaggg tatgctcgtg caagcggtat
     8281 accctatgaa atgggcatga tacgcagcca ttacattggc cgtactttta ttgaacccga
     8341 acagcgcatt cgcgactttg gcgctaaaat aaaatttaac attgtccgtt cagcagttga
     8401 gggaaaacgc atcgttgttg ttgacgattc aattatgcgt ggaaccacca tgcgaaagat
     8461 tatcaagatg ttcagaaaag gcggcgcaaa ggaaatccat gttcgtatct cagcaccacc
     8521 aacacgtttc ccctgttttt acggcataga catacccaca cgttcagaac ttattgcatc
     8581 atcgcatacc attgaggaaa ttcaaaaata cctgcgtgtg gactcacttg cgtacatgac
     8641 ggttgaaagc atgttgaaag ccttagacgt gcatcacgga aaattttgca cagcgtgctt
     8701 tgatggcaat tatcctgttg aatttcaaga agctgatgat taccagaaat gcctttttga
     8761 tgacacggtt gcatgtgggg agtattatta aaaacgcttg acataattgt tacaaatttt
     8821 tctttgttac gtatacaggt gactagctca attggtagag cagcggtctc caaaaccgca
     8881 ggttgggggt tcgagcccct cgtcacctgc atttttattc tatgaaccct catatcacca
     8941 ccatccactt tgatgcaact accttcagta ttgatgacag gcttgtgcac ctattgcagg
     9001 caaaaactca ttacaatgaa ctatctccca ataaaataca tgatactttt ttaaaccaca
     9061 cattttttca tggtggcgat aattccgata ccagtcaagt gcagcaaaca aacctttcaa
     9121 aaggcctgtt gcatatgcta tcacagacca gacagaaagc gcaaccagaa gttttctatt
     9181 gtaaagttga aactcatcac tataatcctt tgcaaacatg cattgttgat gaatatccaa
     9241 tgtatccgtt tatgcttaca ctggggcaac aatttgaaca ctggtgccac gaacatgcac
     9301 tggtgagtga gcagtattac gcacatcttg ctggcatgtg gatgctgcag cactgtgtgg
     9361 agagccttgc acaaattctt gctatggaca ggtattgtac cgtatatcct ggatcatcta
     9421 aagacgttcc gttacaggta aacagacaat tgtatatggt atttagcaaa gaagcccgta
     9481 gcagtggaat cacgcttaat gagcagtata tgttctggcc acttcatacc attgctggta
     9541 tcatactacc acacacgggg gaaaacacct gccagagctg cacgttacta cactgccagt
     9601 tcagaaatat cttgacataa taggtcattt aacaactgat aacaaccatt aaatctatac
     9661 cataaacatt gaaccatgat attaacacat cctgcatact gtaaccgccc cacacgggct
     9721 gaaatttcaa tacctaatct actcaacaat ttttcaattg tgcgggcact ggttgcgcca
     9781 catgtcaaaa ttatggcaat ggtaaaagca aacgcatatg gacatggcat tgtgcgcgtt
     9841 tcacaggaac tgataaaagc aggtgttgat tatttaggtg ttgcctactt agaagaagcg
     9901 ttgtttttac gccagcacgg catatcaaca ccaatactgg tgtgcggtgc cattaacact
     9961 gaacaaatcc ccgaatttat tttaaatgat gttgccatta ctagctcttc actggataaa
    10021 tcaaaggcaa ttcacaatgc agcaaaagca ctgggcaaaa aagcaaaggt gcacctaaag
    10081 atagacacgg gtatggaacg cataggtgta cactggtaca atgcagaaaa ttttattaat
    10141 gaaaccatgt cacttgatgg cattgaagtc actggcattt tttcacatct tgcaaaagct
    10201 gaatctgatg cggattttac aaaacaacag atacaacggt ttgctaccat aattgagtat
    10261 atgcacaaac attctatatg tccaccgtat gtacacctag ctaattcagc agcaatcatt
    10321 aatcatcctg agtcacattt caccatggta cgccctggca ttatgctgta tggctataat
    10381 cccaatggat ataatcccga tattacattt aacggcaaaa agttactgcc ggtaatgaca
    10441 ctgaaaacaa aagtttctta ctttaaagtg gtacctgcac acactggaat tagctacaac
    10501 catttatata ccacgtcaaa acaaacacgt atagtaacgc tgcctgttgg ttacggtgat
    10561 ggctacagtc gtctgctttc caataaaggt gaagttatta ttcgaggaaa aacatatcct
    10621 gtggttggag ctgtatgtat ggaccagatt atggtggata ttggacctga tggtactgcc
    10681 tacaatggtg atgatgtgct actgtttggc cagatggatg gttacagtat tccccttgaa
    10741 tcactatgtc aaaaaattgg caccataacc tatgaaattt taacaaacat tagcccnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncctt caggaatctg ttcaccagct
    10921 attgaatctt caggataacc cgaaccatca tagtatttgt cctgatattt aataatctgt
    10981 gcaatttctt ctaaccgagg aatcttttta agcatatcac atgctataat tggatggttt
    11041 ttatacattg ctatttcatc gtcccctaat tgcaatcccc tgaaagcctt ttttaaaata
    11101 tcatctggta ttgttacaca cccaatctgt gacaacatgg ttgatatatc cagttgccac
    11161 cctttctgaa ttgcaagatt atgtgtaata atttttacaa tagcttttag ccttcgagac
    11221 cgttcaaacg ctattggttt agacaaagct aaaagatcag tcagaacttt aatactgcca
    11281 gctaatgttt tacccagtaa ctccttttca gctgtgatta attcgtattg cctgacacaa
    11341 tcagcaatag ctttttttaa atcatcaggt tcacatggtt ttgttaaaaa tctgaaaatg
    11401 ttccctttat taatgacatc aataactgcc tgcaaatcag cctgccctga aagcatgacc
    11461 ctcacactgt cgggcgatag ttctttaacc ttttccagaa acacaacacc gtccataaca
    11521 ggcatacggt aatcagaaat tattactgaa aattcatttc cttgctgtat atgatccaat
    11581 gcttcggatg ccgaagaaaa agtggttacc tcatattcat ccctcaattg tcgcttgtag
    11641 ccctcaagta catttacatc atcatcaaca agcagtattg tttgcttcat ataattgttc
    11701 cccccaaaaa tatatagatc actgcaacaa ttcctctaat actttcatca attcttctgg
    11761 ttcacagggt tttgacagat atgcgtgtgt gatacttctt gattttgcaa aaacatcttc
    11821 atcaacctgg cctgaaagca aaacacgttt tgcctgggga ttatgctggg ctgctgcttt
    11881 taaaagttca ataccattca cctgaggcat tttataatca accaccacac aatcatattt
    11941 gtgttcttga ataaatgcta aagcctcagt tgctgatgaa ataaaatgca tatcccattc
    12001 attttttttc ttatgtaaaa tcctttttaa tgaattaagt aaatttatat catcatccac
    12061 aaacagtatt attttcataa ttcctccatt taaaagaatc aactacaatt caagagatag
    12121 atcccgtaat ggtaaatata catagaatgt tgtccctttg ccatattctg attctacacg
    12181 tatcatccct ttgtgtttat catagattat tgaatgagct atcgctaaac cctgcccact
    12241 accttttcca acttcttttg ttgtaaaaaa tggatcaaat atttttgatt taatctcttc
    12301 tgatattcca ataccattat cttttatggc aacaattgca taatcagtat tcatatcagt
    12361 tgatatttca atattacctt tttcaattag ctttttatct atagcttcct gaattgcatc
    12421 acgtgcatta ataatcatat ttaaaaatac ctgattaagc tgatttggga agcacggtat
    12481 cattatatca atatgcttga aatcagttgt taaatcagaa atatatttcc attcattacg
    12541 cgttatggtt ataacatctt caagggtctt attaagattt ataataactt ttttttgtat
    12601 gtcaggatga gcaaaatttt ttaaagattt gacaatactc acaattttgc tgattccttc
    12661 aatgctctgt tcaacagcct taggcaactc ttctttgcag tatgtataat caatcttttc
    12721 ttttatagta ttaatttcat tcaataattt ttgcaatcgt tgaggaagtt gttttgctgc
    12781 aaataatact gaagttagtt catcgcatgt gttcaataat gcttcaaggt ctaaaactac
    12841 atctttaata aaatgcaggt tatcattgac atactgtgca ggtgtattga tttcatgtgc
    12901 aatacccgca gctaactggc caattgattg cagtttttgt gcctgcacca tttccgattc
    12961 gctgattctt ttatcagtta tatctctgcc aaagattaaa taccccagca ggataccatc
    13021 ttcatccatt attggattga ttgttatacc cagaaggcgt ttttgaccaa atagcgaatc
    13081 ataccgtaca tcaggaagcg tgattggacg cttttcaact attgacgcag tgatacccat
    13141 atatatctta ttccattccc agggaatgtt aaaaccaata atattttttc caactgcatc
    13201 actactgttt ataccaaata ttttttcagc agtcctgttc cagtgcgtaa taatatcatt
    13261 tcgagaaaca ccaattaata ttgaatcaat tgctgaaagc agatgtttta cctgtccatg
    13321 ggaaatggtt agttcttcct gtgctttttt taatggtgta acatcatcca gagttactaa
    13381 atagctgttt gtatcatcta acgttattgc agcaaaattc atcctgagat atcgctcttc
    13441 ttcaccatac tgtaatttgc attcatagtt ttcaatactc tcccatttag attgtatcag
    13501 ctcataaata ttactttctt taacattcaa tacatcaggc aaatattttc caattaaatg
    13561 tgtatactca cttaagcgta acaactccag cgcttttctg tttgtgtcaa ttatcttaag
    13621 tgtactggca tctaatgtta ataagcccag cggaacataa tcaagcaggt aacggtattt
    13681 gcgttcactc ttttttaatt ctttatatat tgctacctcc tggcttatat caacaaataa
    13741 tgtaagaatg tattttttat ttttttcttc aaaccaggtt ttaataatcc tgaaactaaa
    13801 ttgcctattg ccctgaactg taatggtggt actatattga ttggcttttt tgctctccaa
    13861 taattcagta ttatctaaca agataagtct ttttatttcc tgtgggattt cttgtattga
    13921 ggatatattg atattatgaa agctttttat atgtgtatta catacagtat gtgtaaagta
    13981 ttcattagtt actatgacaa ttccatcttc atcaattatt attaagccag tatttacaag
    14041 gttcagtatt tgttgtattg tttcatatga aatatgattg ttattctcca caatacaaac
    14101 cttattatgg aatgacttct ttattatttg taatactata gcaaaagggt taagtcaata
    14161 ttttttcttt gattgcagga ttgaatttgc taaataattt taaaaacact tccagcaacc
    14221 agggatcata tttactgctt tctttttcca ttatcttaca tgcatcatct ttgctgtaag
    14281 attgtctata tggcctgttg attggtaact atctcattaa tcttaatttt ttattgacat
    14341 aatccttaat ctatacatta tcttcattta ctattatgga gggcttttaa tgattaaaga
    14401 cgcaatgtat tatgattcgt ataaagaaga ccgtgatctg attttagaat ataaccagac
    14461 ttcacttgct gacgttaatt tggtcaacat tattggtatt ggcattgata actgcactcg
    14521 ccagcaggct gtggttaaag tgttacggat ggtagaagag ggtggcgtgc accatgttat
    14581 ggcgcttaac ccttataaac tccatcgtct tacgaccaac aatgacctca acctcattgc
    14641 atctaaagct gatttaaaac tggccgttgg tgcaggcctt ccatgggctg caaaattttt
    14701 gggcagacca ttaaaggaac gaattcattt catgagtttt ctcatggatc taatccgcat
    14761 agccgaaata aaagagctta ccatatttat ggtaggagca aaaccggaga tcattgaaca
    14821 ggcgtatgcc aatataaaaa aatcatttcc caaaatccgc atagtaggcc gacatggagg
    14881 ctactttaat aaagaaagag ctgcaaatgt cattgaagca atgcgcaaat ccgaagcaca
    14941 aataatactt attgggcttg gtttcccaaa agaggataaa tggatttatg aaataaagaa
    15001 ccagttcaaa aatactgtat ttattggcat tggcggtagt atagatataa tctcaggtca
    15061 aaaacgaaaa gcccctgcat actttatgga aaaagggctt gactggtttt atagaattat
    15121 cactcgccca tggcggtatt tgcgtctttt gcgtttgctt cttttttaca tacaggtttt
    15181 gtggcaaaga ttacggaaat aattcatgga ggggcgggat tcaaaaccgc ccatactaat
    15241 tatcgttcat atttgccagg ctttgctttg gtatcacgtg ccttctgtaa ctccgtttgt
    15301 gttacctggc atcttcctaa tgtaaaccac tcaacaattt cctttttgcc acaggtgcgg
    15361 caatggaata cagctttttt atcctgcgtc cattcgttca cttcaacata tcgcgatcca
    15421 caattgcggc ataatattac cgaaacatat tccattaaaa gcctcttttt tattgtattt
    15481 ttacagcaat aactgatata tcttcccgct gagccaggcc ctgcataaat tcttttactt
    15541 ttgttagcaa accatctata cattgagatg gttcatcata ctggcaacat gtgtccatta
    15601 cctgttttaa cccaaaaagg cgtgcattcc ggtttttaca attaacaatt ccatcagtga
    15661 caataataaa catgtcattt gttttaagtt taacaactat tttttttcta tcacgtgtat
    15721 atgaaaaacg catataccgt gcaataccat cacgcactaa gataggatat gcttcaccgg
    15781 tattcatgaa tgtaagtgtc cttgaatgta aattaatcat accataaaac aacgaaacat
    15841 tttgcaaatt ttgttcttta acaacacgca gtgtattgat aaataccggg ctcactgcat
    15901 tttcatatac attcatcaaa ccttttacta ttccattgac cagtgatgca ttgagtgccc
    15961 cgggcacccc cttattgtgg acatcacata acgctatgtt tacagcattt ttttgatata
    16021 acaattcata aaattcacca ccaacttcac gtgcaggaag atatcgtgct gctactttgc
    16081 acaaaccata attctctgaa attgcaggat gtaatgaatg ctggattgaa cgtgcagatt
    16141 ctaattcatg gcgcatgcga gcttcctcaa tagcatgctc aaaaaagatg gcattctgga
    16201 cagcaagtac tgcctgatca gcaaatgcca aaaaaaggtg catatcttca tctgtaaaag
    16261 cagggcgatt aattgggtta attgcctgaa ttactcccag aagcttaccc ttatataaca
    16321 gtgggacaca tataattgag cgtgttgtaa aacctgtttg tttatcaaaa ttgggatcaa
    16381 acctgtcatc tgagtataca tcattgacat atacaccttt tcgctctctg gcaacccatc
    16441 ccgcgatacc ctgccccatt ttaactctgt atttttcctg caacttatcg ccagcctcgc
    16501 ccagtgccac cttaaacaca agttcattgg ctttttcatc atataaaaac aaggtactgg
    16561 cttccgtttc cataatctct tttataatct ccatgattat ggtaaaaagt ttggccatat
    16621 caagtgttga gtttatgatt ctgtttatct caactaactt tgaaaggtta tggagctgtc
    16681 gctcaagata ttccaaattg tatttttttt cattcatgct tttattccct taaaaataat
    16741 tatctattta tttttcggtt ttttatttct taatcatata catacttatg cttttatata
    16801 taaaactgaa caacagtcaa taaaaataat tgtatatagt aaaatattct attagttgtt
    16861 cctgaacccc caggcaaaac attaatacaa aaagatcttc tttacaatgg catttcaaaa
    16921 agtaaaagtt aggatgacca aaatgatgct tgaagtaata ttatataaaa tgatttaaat
    16981 ataatcagta ttgatttttt atgaatcaag taacaatctc acacctaagg ggtttaatac
    17041 tatgtgaaag aacatcatgc tataaaaata gcaccgctaa tggcgttacg gttatagtac
    17101 gtttgatata ttttttaatc aaagcggttg ttttttacaa ttccgtaggg atgtcatttt
    17161 tgtagaaatg tagataacac agcgtcaaac cccagcgggg tgatagtata cgaatataca
    17221 tgtataaaat aaaacagtag ctgaataggt ctaaaaaggg agtattcata caatgatagt
    17281 cagcactgct aacatacctg ttaatataga tagcataatt aaacactata taagaaggta
    17341 caaaaaaaac attattatag gtatgcgtaa tcaatgttat accattgata ctgtgaccaa
    17401 atgtattaaa cctatacatt atgaacatat tgttaaaatt ttaatttcag gaattaaata
    17461 gtgaaaatat ttagtaatca cctataaaaa gtgcttgtat ttacttaata tggtaaccaa
    17521 attaaaagat agcatcatac attacgaatg cgatagtatc tgacaaaaag gtacattgcc
    17581 atgacttacc tcattgatgg ctacaattta atatacaaat ttccccattt agaagaactg
    17641 atgcttcagg ataaccttac tgaagcacgt aaagaacttt tagatatttt aaaaactttt
    17701 gccaggctta ccggaaaacg tatacgtgtt gtttttgatg gtcagaagaa tgtcgaaata
    17761 ccaataagca gtgagaaagt ggccaatatt gacgtttatt actcattaca ctattcagca
    17821 gattttttaa taaaagaatt catacgaaaa gatatccagc cacgtaacac aacagtggtt
    17881 accagcgaca aagatattat tgattttgtt agccgattta aaacacgcac catgaaaagc
    17941 gaagactttg cagattttat aaatcagacc atagaagatt acactgtggc acaaactcca
    18001 gaaaaagaag aaaatcccac attaagcagc gaagaaattg aatactggga aaaacttttt
    18061 ataaaaagaa aaaagtaaat taattttctg ctgctaaaag tgctgccccc agtgccccta
    18121 ttatctgtgc attatctgaa actttaaccg gtgaatctaa aagtttttca atagctgtaa
    18181 caacaccaat attttttgcc acaccacctg tcatcataac gggatttgtt attcctacac
    18241 ggttagccat agccataatt ctcgatgcta ttgattgatg gatgccagca atgatattat
    18301 cacgtgtttc gccttttgct atgagtgata tgacctcgga ttcggcaaat acagtacaca
    18361 ggctgcttat tttagcagga ttattagctt taagcgatag ttccccaaat tcatccagat
    18421 ccacttcaag cgcccgcgcc attacttcaa gaaaacgccc tgtccctgca gcacatttat
    18481 cgttcatagc aaaatctttc accttgccct gctcgtcaat aacgattacc ttgctgtcct
    18541 ggcccccaat atcaatgatt gaacgtatag agttatccag aaagtgagca ccttttgcat
    18601 ggcaggttat ctcggttata ttattatgtg caaatgcaac actttttcgg ccatatccgg
    18661 tagcaacaat cttttctatt ttatccagcg ataagccaag ctcagctaat aactcctgtg
    18721 ttactttttc accagctttt gcagcattaa atccagtaaa tatgatttta ctgccaataa
    18781 tacttttatt atccagaaca accgcttttg tggttatgga accaatatcc attcctaatg
    18841 tcagcatact atatgttttc tcccttaaaa ataaaatata gaacaagcgt tcactcattt
    18901 tttgtcaagc ttatttttac cattcaggct gcagtttatc attatttttt tctctgccaa
    18961 taatatccat tttgtattct aacggcaggt aatatataat agccttaggg gacaggtcat
    19021 cctttttgac aaatccaatg acaaccattt cctgttgatt ttcttttttt acaaatgtcc
    19081 tgaaaataac tggcaatgac atgtcagtaa attgccatcg ctggaactgc ttactggaat
    19141 aaatcatctg tatacggagc ataacttcat caacaatttt atcacacacc agtgatttaa
    19201 gctttgattc atttgtagaa ctatcgccac tacctgattc agagaagata taataatcat
    19261 ctattgattt ttcactaaca ccaatgcact gtgcaatttc aatcttattg taacaattat
    19321 gaataccatg caccgtacaa ataagtgggc ctgtacggat acttttttta gcgcatccgt
    19381 tgcaagcaaa aattatcact actaaagcta ttgtaaaaaa tcgtttcata tacacattat
    19441 ttcaggttca taagtattaa aatgtctttg cccgtatacc agtaactaaa caataaaccc
    19501 gttatctatt tcaggttttc attgaaaaac gccatcatct caggcaatac tgactgacag
    19561 aaattccagt catgcatttt atattttttt tcgacaaagg taaaattgta tccacttttt
    19621 tgttccttct gcaactgttt caattttacc gccagcataa agctctgttc cattggcaca
    19681 cgtgaatcct tattgccatg agctaaaaat actgcaacat tttttaaatt ttttgcatca
    19741 tcaattgcat tgtcaatagc agcccagcgc tgaggattat cattatattt tccatacacc
    19801 gccacaaaca tgttattttt aacatgagcc agcatatcat aatacccaga aagacaggca
    19861 atggcactaa cagtgtcact gtacatttcc cctatgcgaa gtgccccacg tgctccgata
    19921 gaaaatccac atacacccag agagttttta tcaattttta agcctacggt atttttaaga
    19981 taaggaatta atatggttcc aatccataag gctgcgggca gatcattcca ttttactgtt
    20041 gtttcatcaa aaaactggga ttcatacact gttttaggca tgttgggaca tacaataacc
    20101 atattgtaca tattggcata gtaagcaata tttgtattat tttgccagct gttcatagaa
    20161 ccatcataat catgcagagc aataattgta cggctgtttt tggaatattc tttgggaata
    20221 tacagcttaa ctggcacagt ttttgtagaa ttttctaaag caggaacatt aacaataacc
    20281 cagctacctg gtttgagctc tattgcataa acagaatgaa ttgtaagtat tataaaaagc
    20341 agaacctgaa ctataaaagt tcgtttcata acgttatggc cccctatgac aaaatttgtg
    20401 tattttcaat tattttgctc tgacccctta ttgtgaagca accattagat taacacaatg
    20461 aatcataaat agtatacttt ataactttac aacgcatgac tgtgaacata gatattgtat
    20521 gctctgaccc catgttaatt gcaatccaaa cttataatgc agaatgattt ttttcaaggg
    20581 aattttttat cctttcatcg acgttaaaag gctctgaccc caatacataa attttcagat
    20641 taaatcgttg gatagaaatt ttaagaaata cttagcatca aatcatatct ttactgcgta
    20701 aacaacgttt catcagtttc cctcaggctc aaataatcgt tatcggtaac aatgacatga
    20761 tcaagtagct gtatgccaac aatagcacca gcctgcgcca cccgttttgt tatctcaata
    20821 tcattgactg aaggcttgag cacaccagaa ggatgattat gcgcaataat aattgatgat
    20881 gctgcctcac gaattgcatc tctgaacacc tcacggggat ggacaagcgc ctctgaaatt
    20941 gtcccaattg ataccctgga tttttttata agcctgtttt tactatcaag tatcatgacc
    21001 cagaattcct cctgcctgct gcaagcaata tcacccacca aacattccca cacgatacgc
    21061 ggcgagtcaa ttgcttcttc gtaattacgt ggtgctatca aacgtttgcc aagctccata
    21121 gcagctttaa ccctcaccgc ttttacaatg cccattccat taatagcagc tatctcccga
    21181 atacccgcat gataaatacc acttacaccc ccaaaatgct gaataagccc aaaggcacat
    21241 tccataacat cccttccctt tactcctgta ccaagtaaaa tagctaaaag ctctgcatca
    21301 ctcaaatcat caattgattc gccattaata caacgctgaa ctggcaattc acattttgtc
    21361 tgcatagtta ttccttccgt agcaaaattt tgataacggt tactatcgcc ctacttatta
    21421 aaacgcttga gttgtaaaaa ttttaaaaat ttttttaatt tataattttt ttttatgtac
    21481 cgataactat aataggattt ttttatgtca cattatattt tactgttgac aaaattccat
    21541 gatacggaat atattaaatc atagggagtt tgcaatcagg gagggttata tgaaagtcag
    21601 tattgatgct attttacagt ccgcacatca gcttaaaagc actataaatg ttgatgaaca
    21661 caaatcggga aataaaaata aagtaactgg tgataaagtc aacattgaac acagagtaat
    21721 ggcacgcctt gatgaaattc acaatcagat aaaagatatt cagacaacac tttcgcgcta
    21781 ccaggttatt gatgacggta ttcgtatgat attaaatgac aaaaaagcag gtggtaccaa
    21841 tgcgccgaac attatcgatg caacgcaata taataacagg cctgttttaa aagaattttt
    21901 gggcgatagt tacgatgaaa ataccctgac ccataaacaa gatcaattaa agaatcttgt
    21961 tgctgacagt atcacaggtt tgcaacggct ccaagttgaa tttgagaata tcgttgcttc
    22021 tgaccttgca cgcaatgttc aacttaaaga taaaattgat gagattaatt cgctgatacc
    22081 tcgtaacatc aagaatattc agcatctaca tcatcttgac gctgaaaccg tcaagcgtct
    22141 tattcagtga tggcattatc tgtagtgatg ctctgacccc tacttttttt cacacccact
    22201 caagtatctt ggagtacttc tttcgataat actcttcata ctgcattaat ccacgttgtg
    22261 gctctgaccc cttttagtcg taacctgttg gtttgaccct ttttccattt tttacctttt
    22321 cttgtttata ccaatttttt tatacccaat ccac
//
LOCUS       DAORYL010000049        22341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1965_length_22341_cov_13.381719, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000049 DAORYL010000000
VERSION     DAORYL010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 22341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22341
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1965_length_22341_cov_13.381719"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PLV81_09710"
     CDS             <1..578
                     /locus_tag="PLV81_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10410.1"
                     /translation="NKTEQSIKELTEAQKRTEQRVDSLTIKMEELTVALKETQIELRQ
                     LAEDHRTTRRILGNLTHTIGYTLEDTIMKYIPDYAFKEYGVTITTVDRRYITYPDKRY
                     DEINIYSEGTKNGNKVYIIGESKAQLGKKDVESFDKMIKRLQNTLEGEIIGFVVCYSC
                     TPDVEEYVKQEYPYIRIHKSFDFYKYKKSYD"
     gene            complement(632..2311)
                     /locus_tag="PLV81_09715"
     CDS             complement(632..2311)
                     /locus_tag="PLV81_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOM10411.1"
                     /translation="MRIIYCTDVHGAFERVRELLYETIADVYIISGDLIDIPFYNMDS
                     AIRYHELQMYFDNMRKQMGRDDVTVEDFVDELMEMPHITEETAELGAKYQQYTIRARR
                     VMQQKYKVLANIFSFKPQTYIFALPGNYDMDLKYTSLHDRDLHLHWHQVDDQIIAGYG
                     GADVFTAGIPQKYVVPYRAGIGIDDRKNEMYNFFKAVRPTIIVTHQPAHGVLDWLPPV
                     GPTGSPALRTYCDNNPVILCLTGHIHGSWGMKEVEHTLYLNPSPFGDITTVHHDVLEG
                     GFFYQVELDGKTIEHILYRKLYNERVYDLAEYTKKDGEWVETVIDEGRYNAKKKLMNV
                     DMNIQQYSHIPEIELFKEIKNFFRMFQTEESELRVEKMEEIVKRIEPVIEDIAMDVLG
                     SVNVGLSQPSSDIDMVLYLRCGLQCPDNLLSCSCCKDAAEMIRNALQDEYKFEVLDCI
                     DLNEVEKNIKEKNYESETLQRFVAYRSICRPINYRVIAPLEDMLNQDIEFRKEVEGSI
                     RSYFKIFVTTSQHVRSFKKYENRLKTIGIRLPDSIRRKILQYLQSEEDDIL"
     gene            complement(2311..3903)
                     /gene="lnt"
                     /locus_tag="PLV81_09720"
     CDS             complement(2311..3903)
                     /gene="lnt"
                     /locus_tag="PLV81_09720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOM10412.1"
                     /translation="MKIELLRKLSGHYYVLSVPLFYVSFLTPVNLFAPLLSVFAWVCL
                     VPLFVYIRNKPLKDVYITSFITGLFGFGVVYYWMGDFGQALPYGKVVIVALIVPCISV
                     FFATKVLLAEYLSRMFPRLTMILYPSVWIAFDWVQSIGTMAFPWNFLGYTQYPFIHFI
                     QIASITGIFGITFIIVFANISIAELIQAKLCNSNYRPALLACSIVCVVVVVSTGYGFI
                     RLKHHHEYTNSHLRVAMVQSCFSPWENWTRNKYIYLDILKTLTQQAMQHDPDIVVWSE
                     SATLENISYSYFNGNLNHFEEEVLALAAANRVPLLTGEIGIIEDVLKRRYYPQNSMVL
                     INSAGQVVDTYAKIHLVPFGEWFPYGELFPFVNDIIDAFGGSNFIPGDSPRIFTIGSF
                     RCAPLICYENIFHRLCRYYANAGIDFYVNITNLLWTENPVGPMQHFYFSVFRAIENGI
                     WFVSAGNSGFTALIDPYGRITTSVPLMKKTYCVGDMDLSMNTGTVYRLCGDIILYVAW
                     VFILVLCAIVTANKLNAYKRKR"
     gene            complement(3903..5894)
                     /gene="ligA"
                     /locus_tag="PLV81_09725"
     CDS             complement(3903..5894)
                     /gene="ligA"
                     /locus_tag="PLV81_09725"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOM10413.1"
                     /translation="MEKVKKRYDELVALIKKYNYYYYTLDKPLVDDATYDELMKELVR
                     IEEQYPDIVRDDSPTKTVGAVIQTSFNEVRHDPPMLSLNNAMDEADMNDFHERCAKLL
                     GTFDIEYCAELKYDGLAVELVYKNGIYIQGSTRGDGEVGEDVSENIATIKKVPARLQG
                     NVPEYISVRGEVIMRHGEFERLNEQRRQNGEPEFANPRNAAAGSLRQLDPAVTAQREL
                     DIILYGIGKMEPAGKVHTQSQLYELFSQLGLPAPHYYTVGELQDVKAFYTHWMENRYT
                     LDFDIDGVVVKINRFNVRDTMGSTSKAPRWAIAWKFPAREAITVLKSVDYQVGRTGLI
                     TPVANLEPINIGGVIVKRATLHNFQEVKRLDIKIGDVVKVIRAGDVIPKVVEVLRDKR
                     HDVKEIVPPQRCPACNSVLQHEDIYIRCVNPSCPAKEYETLCFFVSKDGMDIEYAGPE
                     LLKRLYEQKKIKTIADIYALTRDDLLQVERMGDKIVDKIIDSINQRREVSLSQFLRSL
                     GIRNVGDHIAKVIARHVRSLDKLQELSPQELMRIPEVGPGVAQSVYDYFHDEESLKII
                     KTMLSNGVVVKDEVVEEASENPFKGKTVVFTGTLKSMSRQEAESLIESLGGKASSSVS
                     KKTDFVVAGTEAGSKLEKAQTLNVRILSEEEFLKMVGRE"
     gene            6146..7135
                     /locus_tag="PLV81_09730"
     CDS             6146..7135
                     /locus_tag="PLV81_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10414.1"
                     /translation="MQLKKEIKTITLKDPFQQFKEAQRVNEIRYCPLTGKSTRVLGLS
                     LKEFATADRSAMIEESQKTCPFCPGLIDALTPKFTPPLQEQYTSGNARCFPNAFPYDE
                     NNAVVTITSQHYVPVNGFTHQMLQEALECALIFLSDIDPERYPYQSINWNYMPVAGSS
                     LVHPHFQIIASSTPSNYYVDILANLEKNPNLFMDYLNTEKSLNERLIEVMGDSTWLIA
                     FAPRGQFDILGILHECTPLHTLTGKLDSVITYIRKTLQFFHHKKIDNFNMAFYSIANN
                     KLFLPHIRLCPRVTFPPYATSEINYLQMLHNDSFTLLKPEIITEEFKAFQFDL"
     gene            7513..8472
                     /locus_tag="PLV81_09735"
     CDS             7513..8472
                     /locus_tag="PLV81_09735"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOM10415.1"
                     /translation="MLQKVLQEYDSKIHLEELLTFGMALFLNILIIVFSHITLAPVIF
                     TINTIIMLLIFATVYAQRYATSKIFTIYRDWYILPLLVAIFMEHNKLVPLINPHDIDT
                     ALIKIDYALFFGNHPTVLLENVLNPFITELLQIVYASFYFLPFTLCLLLYLKERKVEF
                     HIVASTIMLGFYLSYIGYYIFPAIGPRFTLTHLQTKPLEGLWLFNFVHHGLAYLEGVT
                     RDCFPSGHTLVSILTVLLAIKFHKKFSVTATVWALLLIISTVYLRYHYVIDVIAGFFV
                     GLATFRYGPVLARLYIFGSAEISTPVLDYLRNWWDWLSEIRRR"
     gene            complement(8475..9203)
                     /locus_tag="PLV81_09740"
     CDS             complement(8475..9203)
                     /locus_tag="PLV81_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10416.1"
                     /translation="MRRRIKTKPVTIPETKKTYWKRRPSEIRGYQRVFYCNSENGDYI
                     RVDYNFKDKRVRLYLEDSEEGGNPYYAVIDKGRISAERNATSGRPVSLQEKFEKRAEL
                     ISTIPNKDVIKLINKHYGIGESPEEIAARKKKRQEELERTRKRYFKPEEVPSFVKKDT
                     TVVSKQRVRIRDMMDIGIGGATVGGVYFVSLDFIKAGIVAAAFGILLAFVDIFIREKE
                     TLLLKTLLFLVLGIALYIYGYFIL"
     gene            complement(9469..10353)
                     /locus_tag="PLV81_09745"
     CDS             complement(9469..10353)
                     /locus_tag="PLV81_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10417.1"
                     /translation="MGKVQKIIIAAFIIAALVYIFIRSVVFVGDGVLAFVTDTNGQIL
                     GHLHKRVNLIPQGILVTIKANYIPLHGSCNVDIVIPISPLESIKSDHYSIKIPVVINY
                     TLNPEQLTLDLNRLYSDTSYLSQQFEIRLSGSMKDAISRYLYPYYQYNALQVNIHKEL
                     SVAIDHVKESFARDGIIIKEVTPGIIYVPDYTVYTEGKKFLLELLTQEKLRTIQLNAL
                     QQKLKEDELKNAQYLEHLEKISRIIAKNKDILKYIYIDKLADNVRVIVTSDKNIPLDI
                     DSDSESESLKKDFDNFKK"
     gene            complement(10357..10587)
                     /gene="hfq"
                     /locus_tag="PLV81_09750"
     CDS             complement(10357..10587)
                     /gene="hfq"
                     /locus_tag="PLV81_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HOM10418.1"
                     /translation="MGKQTKNLQDTFLNAARKDKVEITIYLMNGVPIKGKVISFDSFT
                     ILVEVDKKQNLIYKHAISTIMPSKPVQYKDEE"
     gene            complement(10712..11623)
                     /gene="miaA"
                     /locus_tag="PLV81_09755"
     CDS             complement(10712..11623)
                     /gene="miaA"
                     /locus_tag="PLV81_09755"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOM10419.1"
                     /translation="MNNKAVVLVGPTASGKTNLSLEIAQDNFEIVSVDSAQVYTYMDI
                     GTGKVSPYHRSLITHYCIDIVNPDYWFTAGDFCREAEHAIQIIQSHGKIPLFVGGTGL
                     YIHAYFYGLAELPKIDSTVKKQLQSELEQKGLKTLYEELLHCDLQWAKRIHPNDTQRI
                     LRGLEVYRATGIPITQFLQSGHKKSNLNILMIALDVDRDVLYKRIENRVDEMIRQGFV
                     EEVKNLRAMGYTQELNSQKAIGYLQLHRVLTNELSLNEAIEQIKQETKHYAKRQVTWF
                     KRYNPVWVAPDNIERIKGIISEWLYKK"
     gene            complement(11714..11914)
                     /locus_tag="PLV81_09760"
     CDS             complement(11714..11914)
                     /locus_tag="PLV81_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10420.1"
                     /translation="MIIPLDKLIAFEDNRYVFSCAAMKVVDKLGNIEGYPESDKNWKV
                     VPNILNLMLNNTIKYEFKEETK"
     gene            complement(11895..12512)
                     /gene="gmk"
                     /locus_tag="PLV81_09765"
     CDS             complement(11895..12512)
                     /gene="gmk"
                     /locus_tag="PLV81_09765"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOM10421.1"
                     /translation="MFSVIISAPSGAGKTTLIEKLLIQNPIFEFAISTTTRVKRAGEA
                     EGKNYYYVSYSEFMQMVDRGDFLEWAKVHDNYYGTTKKEVDRIKANSRIPIFDVDVQG
                     ARSLRNKLQNVISVFIIPPSIKELERRLRHRSTETDHQITVRLNNAIGELREYVNYDY
                     IVVNDDLQKALDELNAILKTIILKREYMDSVVKRLLEDVHDYTIR"
     gene            complement(12515..12766)
                     /locus_tag="PLV81_09770"
     CDS             complement(12515..12766)
                     /locus_tag="PLV81_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HOM10422.1"
                     /translation="MKSTTLINIGFGNAVVAERVIAVITPMSASGKRLREEAKENNML
                     IDATHGRKTRAIIIMDSNHVILSAMQPETIANRLKQSEE"
     gene            complement(12788..13654)
                     /locus_tag="PLV81_09775"
     CDS             complement(12788..13654)
                     /locus_tag="PLV81_09775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOM10423.1"
                     /translation="MESMTGYSFIEKSTEQFTFSIEIKTLNSKYLEVYTNIPKILKSE
                     DDTITNILKKSFERGKIECTIDIYDWVESRPVHIDGKVLLKYYKEVQRSLAKLKIPAS
                     FSVDALLQLDGVVHKERTILSKKSKTDIYNTLQKSIESAIKMRKKEGKSIKKDLELSL
                     RVIENACHDIQKQANTVVEYQFKRLKKSLESLLHSKVDENRIYTEIAILADKQDINEE
                     LSRLNDHIGKFKDIMAQEGQMGRQLDFLAQEMFREINTIGSKSNSTLIAHKVVEVKNH
                     IDKIREQCRNIV"
     gene            complement(13667..14146)
                     /locus_tag="PLV81_09780"
     CDS             complement(13667..14146)
                     /locus_tag="PLV81_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOM10424.1"
                     /translation="MEFKLITVPIADYKISQSPDILRTILGSCVGICLYDPESRIGGL
                     AHIMLPENNDKSTNPKKYADSAIALMIDELRKKGANIEHLVAKIAGGASMFKMPENSF
                     IGSIGINNVQKVREILDNYRITIISQDVFGDYGRTVDFFLETGKLKIKSLGREDKEI"
     gene            complement(14136..14996)
                     /locus_tag="PLV81_09785"
     CDS             complement(14136..14996)
                     /locus_tag="PLV81_09785"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOM10425.1"
                     /translation="MIDFSNIKRLNEDEFKKFAQLIYDESGIFLKESKITLLSNRLRK
                     RLKALNLSEFSDYYNYITSLKDKSKEIEELLDVVSTNETYFFRNERQFDALKDVCIPE
                     IVKKKKDKNLRIWSAGCSTGEEPYTIAICVMEARSILLGWNISIIATDIAPSVLEFAK
                     IGRYTGRRIEKVPPEILKKYFTQMPDNPEVYEVKDELKKLVSFSYLNLFKNPYPENVD
                     IIFCRNVMIYFDRARQKELIAHFYKVLNPWGYLFIGHSETLHALSDDFQYVKIMDSPV
                     YVPKEKVNGI"
     gene            complement(15028..15870)
                     /locus_tag="PLV81_09790"
     CDS             complement(15028..15870)
                     /locus_tag="PLV81_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10426.1"
                     /translation="MQLVILIMVNILIASIFYLIISLKLERSATEFRSKRLRKEMEDL
                     INEFNHTAERNLALLEYKINILRELLKRADALPGLDITINDESQIKPGSKKSRKKINR
                     NEENIFAEHIQDKEDIQTILTDPLFDISKKTGKELHDNAIVYLEREKTQQFSENKSQV
                     PTITQQLSEIARVVLHKFSQYIESRYTKKANINIPVHPEDVSHSVIEEYDTSQAKCDE
                     KLADVVIPADEEELITLYNNADDKYLMIATLYSQGYPLQVLSKISGIPIGEISLILNL
                     SMKK"
     gene            complement(15889..16200)
                     /locus_tag="PLV81_09795"
     CDS             complement(15889..16200)
                     /locus_tag="PLV81_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10427.1"
                     /translation="MAIKPIDLQTNIAQIIEVARGEHARNAALVEQQHILSKESQEKT
                     NIQKEKVEENKKAERTIILNEEKQFKKGKQFKEGEKKEKQEQPEVLEDTKIGRIIDVK
                     K"
     gene            complement(16225..18201)
                     /locus_tag="PLV81_09800"
     CDS             complement(16225..18201)
                     /locus_tag="PLV81_09800"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOM10428.1"
                     /translation="MKNLKDLMKTIQDDESSENEIEVYADSVKQALELAAGELGVDIS
                     ELDYQILEKGTSGFLGIGRQPYRVLVRPIITEKEEEEDFAEIEQKLHSIHEDDLRIKD
                     VPKDVDGSAKIRVTKTGIWLTITPPKGNGRRVDLSDISNKIYALRIQNADMTKVEKEV
                     RKPSGKPVKIGDWIPNPEYDGTMTVEITDDEMKAFVHFVPPRFCGRHMDVDDVIEALK
                     RAGVVTGIKEERIAEYLDRMDYSQPLLAAEGTPPRHGRDAYIDYKVRIDKSGIQLEED
                     EKGRVDFKDLDLIENVVVGQVLAVKVPAEEGVPGRTVTNRIIPARSGKDIQIRYGKGT
                     ILSQDGTELTAEINGQVVYQNGKLSVEPVFIVKGDVSTETGDVVFLGSVIVSGSVQDN
                     FTVKAAGNIEIKGTVQKANLEAEGDIIIRQGIMGREGARIESTGGSIIAKFIQTATVV
                     AEKDVIVAEGIVHSNVDAGNRIICNGRRARIVGGLIRAGEEVNSKYVGSDSFTKTIVR
                     VGMNPKVLQQLTELEENKRVVSEEFDKVRKDVTTLTVQKNNAGGHLPKDREELLGKLI
                     GQKQKLEQRLNEINLSLEELKAYLNMLEQKGKVCVEKTIFPGVEIYIKDKRFNVKDPY
                     NYVKFALEGADIRISEYEPPDLEESQKLMIRRRR"
     gene            complement(18255..19055)
                     /gene="whiG"
                     /locus_tag="PLV81_09805"
     CDS             complement(18255..19055)
                     /gene="whiG"
                     /locus_tag="PLV81_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004421398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HOM10429.1"
                     /translation="MANKKFQEFDAIDEDELWKRFAKTHDQAIRDYFVVKYAPLVKYV
                     AGKIAMGMPSSIEFDDLVSYGVFGLIDAITKFDPERGIKFKTYAMTRIRGAIFDELRS
                     IDWIPRSIRQKAKQIEQAIADLENKLGRTVEDEEIAKELGITVEEFQSLLNKLSGTAM
                     LSLNDIWYVGDENDELSILETLEAPDTLNPDVMIEKEEIRDYIVDAIKKLPDKEKKVI
                     VLYYYEDLTLKEIGEVLEVTESRVSQLHTKAIMRLRGRLGRIKSNIVG"
     gene            complement(19074..19607)
                     /locus_tag="PLV81_09810"
     CDS             complement(19074..19607)
                     /locus_tag="PLV81_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10430.1"
                     /translation="MFRISLEGKVSIIFGILAFFVSLLFGIIAGNKISMLFSRVLFFT
                     VIYAAIGYGSLLIIKRLVPEVYEILNQSFEFSQKNVSQEEYVSVKPDETSEVREESAT
                     RDEEYTAETSVSGTSDSSYNEVFTPLEAKDFTNLSTKDSKPKKMGKHIVLDDKKIQYE
                     PKIMAEAIRTMLKRDKD"
     gene            complement(19737..20618)
                     /locus_tag="PLV81_09815"
     CDS             complement(19737..20618)
                     /locus_tag="PLV81_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HOM10431.1"
                     /translation="MDQAANLRRLVIQNNAARRTKTIAITSGKGGVGKTSVAVSLAIA
                     LAKRNYSVTLLDADLGLANVNVVLGIIPKYNLYHVIKGKKKLEEIIIDVPEGIKIIAG
                     ASGFHQLANLDVKQREYFIKALSELNDDDYMIIDTGAGISQNVLSFLVASDEVIVVTT
                     PEPTAITDAYGTIKAIAANDPDKTVKLLVNRAQTVTEAKKVAQRVINIAGQFLNIKVD
                     NLGFIFDDMYVAKSIRNQKPFIVSYPKSKASTCIEIIADRVANIESDTDKGTGMLSFF
                     RRFFGHYESENEEDSLQ"
     gene            complement(20629..21939)
                     /gene="flhF"
                     /locus_tag="PLV81_09820"
     CDS             complement(20629..21939)
                     /gene="flhF"
                     /locus_tag="PLV81_09820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HOM10432.1"
                     /translation="MRYVKIKARTYNEAMMKLKMEHGDDAIPISHKNVKEGGLFNTGL
                     FARDFVELTAAIPDVKPAKLKPKKNIDLTVGTNDNVNELLKQVIEKPETEPHKKPHTG
                     LEQLLTQSILQSRTQQEEVKPVVKTVEEQKVETTDSSYIKLEKEFNELKALLKNLVET
                     KQERLSSDICLDNGDTYFKPFKEILRKNDMDDEECTMLLDEVKRNVSSEDMKDKYKIE
                     KTLRELIKSRIVVSGPIDRGKRKKVLMFVGPTGVGKTTTMAKLGAILSLRQGLKVSFI
                     TIDNYRIAATEQLKKYAEIMRIPIHVVTDPAEFKEIVENEKADIIMVDTSGRSHRNDL
                     KIAEIKNFADNCTCEIVKILCVSANTKRSDLSEVFKAYDILHIDNVIITKVDETSYIG
                     NVISVADKYNKPISYITNGQEVPNDIAIAEPDMMVNMILESTMR"
     gene            complement(21976..>22341)
                     /locus_tag="PLV81_09825"
     CDS             complement(21976..>22341)
                     /locus_tag="PLV81_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HOM10433.1"
                     /translation="VALARQICREYADKNNTLSVITVDPEIETIIRSSIHEDPVEGRI
                     VSLDPDTHREVLESLLGSYSKARSAGFAPVYLVSPHIRSVLFTLLEREVPDPVVLSYN
                     EIVPEIRVNVIGTALLRAA"
BASE COUNT         6627 a         4719 c         3353 g         7642 t
ORIGIN      
        1 aaaataaaac cgaacagagt atcaaagaat taaccgaagc tcaaaaacgt actgaacaac
       61 gagttgattc tcttaccatc aaaatggaag aattgactgt tgctttaaaa gaaactcaga
      121 ttgagttaag gcaacttgct gaagatcaca gaacaaccag aagaatattg ggcaatctga
      181 cccacactat tggttataca cttgaagata caattatgaa atatataccc gattatgcat
      241 ttaaggaata tggtgttacc atcaccaccg ttgaccgcag atatattaca tatcctgata
      301 aacggtacga tgaaattaat atttattctg aaggcactaa aaatggcaat aaggtctaca
      361 ttattggcga aagcaaagca caattaggca aaaaagatgt agaaagcttt gataaaatga
      421 tcaaacgttt acagaataca ctggaaggcg aaattattgg ttttgtggtc tgttacagct
      481 gtacacctga tgtggaagaa tacgttaaac aggaatatcc atatattcgt attcataaaa
      541 gttttgattt ttacaaatac aaaaaaagtt atgattaata tttattgtat actactgtag
      601 cttagagcag cataagtata cacttcattt tttataaaat atcgtcttct tcactttgca
      661 aatactgcaa aatctttctt ctaattgagt cgggaagcct gataccaatc gtttttaatc
      721 tgttttcata ctttttgaac gaacggacat gctgtgaagt ggtaacaaaa atcttaaaat
      781 atgaacgtat gctcccttca acctccttac gaaattctat gtcctgattt agcatatctt
      841 caagaggtgc aataacacga taatttatgg gacgacagat agagcggtat gctacaaacc
      901 gctgcaaagt ttccgattcg tagtttttct cttttatatt tttttcaact tcgttcaaat
      961 caatacaatc aagcacttca aatttatatt catcctgcaa tgcatttcgt atcatctctg
     1021 ctgcatcttt acagcaacta cacgataaaa gattatcagg gcattgcaat ccacaccgca
     1081 aatacagtac catatcgata tccgaacttg gctgtgataa tccaacattg actgatccaa
     1141 gcacatccat tgcaatgtct tcaatcacag gttcaatacg cttaactatc tcttccattt
     1201 tttctacacg caattctgat tcctcagttt gaaacatcct gaaaaaattt tttatttctt
     1261 taaataactc aatctcaggt atatgtgaat actgctgaat attcatatca acattcatta
     1321 atttcttctt tgcattatat cgtccttcat caataacggt ttctacccac tcaccgtctt
     1381 ttttagtata ctccgctaaa tcatacacac gttcgttata taattttcta tacagtatgt
     1441 gctctatagt ttttccatca agttctacct gataaaaaaa ccctccctca agcacatcat
     1501 ggtgcacggt ggtaatgtcc ccaaatggcg atggatttaa atacaacgta tgttcaactt
     1561 ccttcatacc ccatgatccg tgaatgtggc cagttaaaca taaaataaca ggattattgt
     1621 cgcaataggt tctgagcgca ggtgaccctg ttggaccaac gggtggcagc cagtcaagca
     1681 ctccatgggc aggctgatgt gtcacaatga tagttggtcg tactgctttg aaaaaattgt
     1741 acatctcgtt ttttctatca tcaataccaa tgccagctct atagggcaca acatactttt
     1801 gtggtatacc tgccgtgaat acatcagccc caccatagcc agcaataatc tgatcatcga
     1861 cctgatgcca gtgcagatgc aggtccctgt cgtgaagcga tgtgtatttt aaatccatat
     1921 cgtaattgcc aggcagtgca aaaatatatg tttgcggttt aaagctaaaa atatttgcca
     1981 acaccttata cttttgctgc atcactctgc gtgcacgtat ggtgtactgc tgatattttg
     2041 cacccagttc tgctgtctct tccgtgatat gcggcatttc cataagctca tcaacaaaat
     2101 cttccaccgt gacatcatca cgtcccattt gcttacgcat attgtcaaaa tacatctgca
     2161 actcatggta gcgaattgcg ctgtccatat tgtaaaatgg aatgtctatc aaatcaccag
     2221 agatgatata cacatcagct attgtttcat acaatagttc tcgtacgcgt tcaaacgcgc
     2281 catggacatc ggtacagtag attatacgca tcagcgtttc cttttatatg cattaagctt
     2341 attagcagta actatcgcac acaatacaag aataaaaacc catgcaacat acaggataat
     2401 atcaccacac aggcgataca ctgtgcctgt attcatactc aaatccatat cacccacaca
     2461 gtatgttttt ttcatgagcg gcactgaagt agtaatacgc ccatacggat ctatgagtgc
     2521 cgtaaaacct gaattgcccg cactgacaaa ccatatgcca ttttcaattg ctctgaatac
     2581 tgaaaagtaa aaatgctgca tgggaccaac tggattttcg gtccataaaa gattagtgat
     2641 gtttacataa aagtcaatgc ctgcatttgc ataatacctg cataatctgt gaaaaatatt
     2701 ttcatagcat ataagtgggg cacatctgaa tgaaccaatg gtaaaaatac gcgggctatc
     2761 accaggaata aagtttgaac caccaaatgc atcaatgatg tcattcacaa acgggaacag
     2821 ttccccgtat ggaaaccact caccaaaggg tactaaatgt attttggcgt acgtgtcaac
     2881 aacctgacct gccgagttga taagcaccat gctgttttgc ggataatacc gcctttttag
     2941 cacatcttca ataatgccaa tttcacctgt tagcaggggt acacggttgg ctgcagccag
     3001 cgccagcact tcttcttcaa aatgattcag attgccattg aagtagctat atgaaatatt
     3061 ctctaacgtt gcggattctg accacaccac aatatcagga tcatgctgca tagcctgctg
     3121 tgttaacgtt ttaagaatat ccagataaat atatttattt cttgtccagt tttcccatgg
     3181 cgaaaaacat gactgcacca tggcaacacg caggtgtgaa tttgtatact catgatggtg
     3241 cttgagtctt atgaaaccat aacccgttga taccaccaca acaacacaca caatactgca
     3301 cgcaagtaat gcagggcgat agttactgtt acaaagcttt gcctgtatta attcagcaat
     3361 gctgatattt gcaaacacaa tgataaacgt gataccaaag attccagtaa ttgacgctat
     3421 ctgaatgaag tgaataaacg gatattgcgt ataacctaaa aagttccagg gaaatgccat
     3481 ggtaccaatg gactgtaccc agtcaaatgc aatccacacc gatggataca gtatcatagt
     3541 aagacgtgga aacattcgcg acagatattc agcaagtaaa acctttgtag caaaaaagac
     3601 cgatatgcac ggaacaataa gggctacaat tacaaccttg ccatacggca acgcctgtcc
     3661 aaaatcaccc atccagtagt aaacaacacc aaacccaaaa agtcctgtaa tgaatgatgt
     3721 tatgtaaacg tctttcagcg gcttattacg tatgtacaca aacagaggaa caaggcacac
     3781 ccaggcaaat actgacaaaa gcggcgcaaa taaatttaca ggtgttaaaa acgatacata
     3841 gaacaaaggt acactaagca catagtaatg ccctgaaagc ttacgcagca attcaatctt
     3901 cattattccc ttcccaccat cttcaaaaat tcttcttcgg atagaatgcg cacattgagc
     3961 gtttgtgcct tttcaagttt ggaaccagcc tccgtgcctg caacaacaaa atcagttttt
     4021 ttacttactg agcttgaagc ctttcctccc aatgattcaa tcaggctttc tgcctcctgc
     4081 cttgacatac tcttcagcgt tcctgtaaac acaactgttt ttcctttaaa tggattttca
     4141 gaggcttctt ccacaacttc atcttttaca acaactccat tgctcagcat cgttttaata
     4201 atcttcaacg actcctcatc atggaagtaa tcatacactg actgtgcaac acctggtccc
     4261 acttcgggta tcctcataag ttcctgtggc gatagttcct gcaatttgtc cagtgagcgt
     4321 acatggcggg caataacctt tgctatatgg tcaccaacat tacgaatacc cagtgatcgc
     4381 aaaaattgtg acaggctgac ctctcttcgc tgattgatgg aatctataat tttatccacg
     4441 attttatcgc ccatccgctc aacctgtaaa aggtcatcac gtgtcagtgc atagatatcg
     4501 gcaattgttt ttatcttttt ctgttcatac aaacgtttga gtaattcagg accagcatat
     4561 tcaatgtcca ttccatcctt tgaaacaaaa aaacacagtg tttcatactc tttagcaggg
     4621 cacgatggat tgacacagcg tatatagatg tcttcatgct gaagtactga attgcaggca
     4681 gggcaccgct gtggaggaac tatctcctta acatcatggc gtttgtcccg taacacttca
     4741 actacttttg ggataacatc accagcacgt attactttaa ccacatcgcc aatttttata
     4801 tccagccgct taacttcctg aaagttatga agcgtagcgc gtttgactat cactccacca
     4861 atattaatgg gctctaaatt tgcaacaggc gtaataaggc ctgtacgccc aacctgatag
     4921 tcaactgatt ttagcaccgt aattgcttcc cgtgcaggaa acttccacgc aattgcccat
     4981 cgtggtgctt ttgaagtgct gcccattgta tcacgcacat taaatcggtt tatcttcacc
     5041 acaacaccat caatatcaaa atcaagcgtg tacctgtttt ccatccagtg cgtataaaag
     5101 gctttaacat cctggagctc accaaccgta taataatgcg gtgcaggtaa tcccagctgg
     5161 ctaaataact catacaactg gctctgtgtg tgtacttttc ctgcaggctc cattttccca
     5221 atgccataca gtataatgtc aagctcgcgc tgcgctgtaa ccgccgggtc aagctgtcgt
     5281 agcgaaccag ccgctgcatt acgtggattg gcaaactcag gctcaccgtt ttgcctgcgc
     5341 tgctcattca ggcgctcaaa ctcgccatgg cgcatgatga cttcaccacg aacacttata
     5401 tattcaggaa catttccttg taagcgtgca ggcacttttt taatggtggc aatattttct
     5461 gatacatctt caccaacttc accatcacca cgggttgacc cctgtatgta tattccattt
     5521 ttatacacaa gttcaacagc aagcccatca tattttaatt cagcacagta ctcaatatca
     5581 aaagtaccta acaacttagc acaccgctcg tgaaaatcat tcatatcagc ctcatccatg
     5641 gcattgttta aactcagcat tggcggatca tggcgcactt cgttaaatga tgtctgaata
     5701 acagcaccaa cagtttttgt aggcgaatca tcacgcacaa tatcaggata ctgttcctct
     5761 atacgtacaa gttccttcat taattcatca taggtggcat catcaaccag aggtttatca
     5821 agcgtatagt aatagtagtt atattttttt attagtgcaa caagctcatc atagcgcttt
     5881 tttacttttt ccattgttat caccccttct aaaaacgcaa ttttgaataa agtattatcc
     5941 tgattaacga aattatgaaa gatggcagca ttgccatgtt gttttaatgt aaagttttat
     6001 gtaactccaa tcaacaaaac tcatttgcca tgataaaaaa agtagtttaa caccatgtaa
     6061 gataaaaaaa ttattgtaaa atacattaga cgttttaaaa agcaaaatag tatttagatt
     6121 atgtattaat caatagggga aaagtatgca actaaaaaaa gaaatcaaaa caataacact
     6181 gaaagaccca ttccagcagt ttaaagaagc acagcgagtt aatgaaatac gatattgtcc
     6241 attaacaggc aaaagtactc gtgttttagg tttatcctta aaagaatttg ctactgctga
     6301 ccgctctgca atgattgaag aatcacaaaa aacgtgcccc ttttgccccg ggcttattga
     6361 tgctctgacc ccaaaattta ctccaccact acaggaacaa tacactagtg gcaatgcccg
     6421 atgttttccc aatgcgtttc cttatgatga aaataatgca gtggtgacca taaccagcca
     6481 gcactatgta cccgttaatg gttttaccca tcagatgttg caggaagcct tagaatgcgc
     6541 actgatcttt ttgtccgata ttgaccctga acgatatccc tatcaatcaa taaactggaa
     6601 ttatatgcca gttgcaggca gctcccttgt tcatccgcac ttccagatca tagcatcttc
     6661 cacaccatca aattattatg ttgatatact ggcaaacctg gaaaaaaatc ccaatctttt
     6721 tatggactac cttaacactg aaaaaagcct gaatgaacgg cttatagaag ttatgggcga
     6781 tagtacctgg ttaatagctt ttgctccaag gggtcagttt gacatacttg gcatactcca
     6841 tgaatgtaca ccactacata cattaacagg aaaactagat tcggtaatta catatatacg
     6901 caaaacattg cagtttttcc atcataaaaa aattgataac ttcaatatgg ctttttacag
     6961 tattgcaaac aacaaattat ttttaccgca tatacgacta tgcccacgtg ttacatttcc
     7021 accatatgca acaagtgaga tcaactatct ccagatgttg cataatgatt cattcacatt
     7081 gctcaagcct gaaataatta ccgaagagtt taaggcattt caatttgact tataagtgaa
     7141 cttctaaaaa ctgttttttt aggatgttcc cttatgggca caatagatgg gataaaaccg
     7201 ctttcttttt attgcatccg taatgatgaa agcggttttt tagaggtgcc cataatattt
     7261 cttgacaaat gattaaaatt ctgaccaaaa ttgttcttgt ctggcattta tctatacaac
     7321 aggctttatc agggaaggta atgtgtattc ctgatgattg aagaaagtac aatagtatta
     7381 aatcgtaaat atttagtttt tgtataatat ttcatgcaaa atattgtcat tttataacaa
     7441 attgtatata ttattttata agataataga aaactatacc atacatacat atattgggat
     7501 aagaggccgg atatgctaca gaaggtgtta caggaatacg attcaaagat tcatctggaa
     7561 gagttgctga catttggcat ggcacttttt cttaatatat taattatagt tttttcgcac
     7621 attacgctgg caccggtaat ctttaccatc aataccatca ttatgttact tatttttgca
     7681 acagtatatg ctcagcgtta tgccacttcc aaaatattta ctatttacag agattggtat
     7741 atattgccac ttcttgtagc aatattcatg gagcataata aactggtacc ccttattaac
     7801 cctcatgaca tagatactgc cctgattaaa attgactatg cactattttt tggtaatcat
     7861 cctaccgtat tactcgaaaa tgtcttaaat ccctttataa ctgagctatt acagatagtt
     7921 tatgcaagtt tctatttttt accatttaca ctatgcttat tattatatct taaagaacga
     7981 aaagttgaat ttcacatagt tgcttccaca ataatgctcg gattttactt atcgtatatt
     8041 ggctactata tattcccggc tataggccca aggtttacac tgactcatct tcagacaaaa
     8101 ccacttgaag ggctatggtt attcaacttt gtccatcatg gactggcata tttagaaggt
     8161 gttactcgcg attgtttccc cagtggccac acattggtat caattttgac agtacttctt
     8221 gccataaaat tccataagaa atttagcgtt actgcaacag tatgggcact acttctgatt
     8281 atatcaacgg tgtatctgcg ctatcactat gtcattgatg ttatagcagg attttttgtt
     8341 ggcctggcta catttcgata tggccctgta ctggcacggt tatatatatt tggcagtgca
     8401 gaaatttcta ctccagtact ggattattta cgcaactggt gggattggct ttcagaaata
     8461 agaagacgat aattttacaa aataaaataa ccatatatat ataaagctat tccaagaact
     8521 aaaaataata atgtcttgag taatagtgtt tctttttcac gtataaaaat atctacaaat
     8581 gccagaagta ttccaaaagc tgcagcaaca atacccgctt taataaaatc taaactgaca
     8641 aaatacacac caccaacagt tgcaccacca attccaatat ccatcatatc acgtattcta
     8701 actctttgtt ttgaaacaac tgtggtatct tttttaacaa acgatggtac ttcttctggt
     8761 ttgaaatacc gttttcgggt tctttccagt tcttcctgtc gttttttctt tctagcagct
     8821 atttcctcag ggctttctcc tattccataa tgtttgttta taagttttat gacatcttta
     8881 ttgggaattg tggatataag ttcagcacgc ttttcaaatt tttcctgcaa acttacaggc
     8941 cttcctgatg tagcattcct ctcagcagaa attcgaccct tatctatcac tgcataatag
     9001 gggttcccgc cttcttcact atcttctaaa tagaggcgaa cccttttatc tttaaaatta
     9061 taatcaaccc gaatataatc tccattttcg ctattgcagt aaaaaacgcg ctgatagccc
     9121 cgtatttctg atggccttcg tttccagtag gtcttctttg tttcagggat tgtaaccggt
     9181 tttgttttta ttcttctacg cattatataa cctgattata tagtaaaata gaaaagagat
     9241 tgacctgtcg ttttgccaat ctctcttcca tttacattat cgtaatttct tcttgtgacg
     9301 attaagtctt cttcgttttt ttcgtttgtg attagcaatc tttctgcgtt ttcgcttcct
     9361 accacagggc ataatattta actctcctta tataaaaaat atatcaatat aatactttat
     9421 gaagtatatc caattattaa tatactgcga aattaagtaa ataatccact attttttaaa
     9481 gttatcaaaa tctttcttta aagattctga ttcgctgtca ctatctatat ccaatgggat
     9541 atttttatcc gaagttacta tcaccctcac attatcagct aatttgtcaa tataaatata
     9601 cttcaatata tccttatttt tagctataat ccttgatatt ttttccaggt gttccagata
     9661 ctgtgcattt tttagttcat cttccttaag cttttgttgc aatgcattta attgtatagt
     9721 acgtaatttc tcctgggtaa gcaattcaag caaaaatttc ttaccctccg tataaacagt
     9781 ataatctggc acatagataa tacctggagt aacttcttta ataataatac catctctggc
     9841 aaaggattct ttcacatgat ctatcgccac agatagttct ttatggatat ttacctgcaa
     9901 ggcgttatac tggtaatacg gatatagata tctggaaata gcatctttca tgctccctga
     9961 caaccgtatt tcaaattgct gtgatagata tgacgtgtcc gaatacagcc tgtttaaatc
    10021 cagtgttaat tgttcaggat tgagtgtata attaataaca actggaattt taatagaata
    10081 atggtcactt tttattgatt ccagcgggga gatagggatt acgatatcca cattgcatga
    10141 accatgtaag ggaatatagt ttgccttaat ggtaacaaga atcccctgcg gaatcagatt
    10201 cacccttttg tgtaaatgtc caagtatctg accatttgta tcagtaacaa aagccaatac
    10261 tccatcacca acaaagacaa cagatcgtat aaaaatataa accaaagcag ctataataaa
    10321 tgctgcaata ataatttttt gcaccttacc catgaattat tcttcatctt tatactgaac
    10381 aggctttgac ggcataattg ttgatattgc atgtttataa ataagattct gctttttgtc
    10441 aacctcaact aaaattgtga agctatcaaa gcttatcacc tttcccttga ttggaacacc
    10501 attcataagg tagatagtaa tttccacttt atctttgcgt gctgcattta gaaaagtgtc
    10561 ctgtaaattt tttgtttgtt tgcccattgt gcaccctctt tatgtatttt aatgttaata
    10621 tttattttat tgtaactatt tgttactact taacacttac ttatcattga atagtaataa
    10681 tatcaatatt tttttctaat tattttaata tctacttctt atatagccat tcagagatta
    10741 ttccctttat tctttcaata ttatctggtg ctacccatac tggattgtat cgcttaaacc
    10801 aggtgacctg ccgtttggca taatgctttg tttcctgttt tatttgctca attgcttcat
    10861 taagcgatag ctcatttgtt aacaccctgt gaagttgtaa atatcctatt gccttttgag
    10921 aattgagttc ctgtgtatat cccatagctc ttaaattttt tacttcctcc acaaagccct
    10981 gtctgatcat ttcatcaacc ctgttttcaa tccgtttgta taatacatca cggtccacat
    11041 ccagcgcaat cattaaaata tttaaattac tctttttatg ccctgattgt aaaaattgtg
    11101 ttattggtat accggttgct ctgtaaactt ccagaccacg caatatacgc tgagtatcat
    11161 tgggatggat gcgctttgcc cattgtaaat cacagtgcaa tagctcttca taaagggttt
    11221 tcagcccttt ttgctctaat tcactttgca attgtttttt tactgttgaa tcaattttgg
    11281 gcagttcagc taacccataa aaataagcat gaatatacaa tccagtacca ccaacaaaaa
    11341 gcggtatttt cccatgagat tgtataatct gtattgcatg ctcagcttct ctgcaaaaat
    11401 ctcctgcagt aaaccagtaa tccgggttaa caatatctat gcaataatgt gttatcaaag
    11461 atctatggta gggggaaact tttcccgtac caatatccat atatgtataa acctgagctg
    11521 aatcaacaga aactatctca aaattgtcct gtgctatttc aagcgataga tttgtttttc
    11581 ctgaagctgt tggaccaaca agaacaacag ctttattatt caaatcattt atcccaaaac
    11641 atcaaaacct gtatgcatta tcatcaacaa tttaagcatg atgtcgtatc agaattggca
    11701 agcactattg ccgttattta gtttcttctt taaattcata tttgattgtg ttattaagca
    11761 tcaaattcaa tatgtttgga acaaccttcc agtttttatc actttctgga tagccttcaa
    11821 tattgcccag cttatccacc actttcattg ctgcacagga aaatacatat ctgttatctt
    11881 caaatgctat taatttatct aatggtataa tcatgtacat cctccagtaa cctttttact
    11941 acagaatcca tatattcacg tttaagtata attgtcttta aaatagcgtt gagttcatcc
    12001 agggcttttt gtaaatcatc attaactact atataatcat aatttacata ttcccgcagt
    12061 tcaccaattg cattatttaa tctcaccgtt atttgatgat ctgtctctgt tgaacggtgc
    12121 ctcaaccttc tttccagctc ttttatagat ggtggaataa taaatactga tataacattt
    12181 tgtaatttat tacgtaagct tctggcaccc tgaacatcca catcaaaaat aggtattctt
    12241 gaatttgctt taattctgtc aacctctttt ttagtggtac cataataatt atcatgaacc
    12301 ttggcccatt ctaaaaaatc acccctgtcc accatctgca taaattcact ataacttaca
    12361 taataataat ttttcccttc agcctcacct gcccgtttaa cccgggtcgt agtggaaatt
    12421 gcaaactcaa aaataggatt ttgtatcaag agcttctcaa ttaacgttgt ttttcctgca
    12481 cctgatggtg ctgagataat tactgaaaac atgtttactc ttcactttgt tttaatcggt
    12541 ttgcgatagt ttccggctgc attgctgata atatgacatg attgctatcc ataataatta
    12601 tagcacgtgt ttttcgccca tgagtagcat ctatcaacat attgttttct tttgcttctt
    12661 cacgcaaacg cttacccgaa gcagacatgg gagtaataac agctataaca cgctcagcta
    12721 caacagcatt gccaaaccct atgttaatca gcgttgttga tttcatatac tcatccttat
    12781 cctcatatca aacaatattc ctgcattgtt cccgtatttt atcaatatga tttttaactt
    12841 caacaacttt atgcgctata agtgtactgt tagattttga gccaatggta tttatttccc
    12901 tgaacatttc ctgtgcaaga aaatccagct gtcttcccat ttgcccttcc tgtgccatta
    12961 tatctttaaa tttaccgata tgatcattca gtcgtgacag ttcttcattt atatcctgtt
    13021 tatccgctaa aattgcaatc tcagtataaa tcctgttttc gtcaaccttg ctgtgcaata
    13081 aggactctaa agattttttt aatcttttaa actgatattc aacaactgta ttcgcctgct
    13141 tttgaatatc atggcatgca ttttctatga ctctgaggga taattccagg tcctttttta
    13201 tggatttgcc ttctttttta cgcatcttaa ttgcagattc aattgacttt tgtaaggtat
    13261 tataaatatc agtttttgac ttttttgaaa ggattgttct ttctttatgt accacaccat
    13321 caagttgtaa taatgcatca actgaaaaac tggcgggtat ttttaacttt gcaagtgatc
    13381 tctgaacttc cttataatat ttcagcaaaa ccttgccatc aatatgaaca ggtcgtgatt
    13441 caacccaatc atatatatct atagtacatt caatttttcc acgctcaaaa ctttttttca
    13501 gtatattcgt aatagtatca tcttccgatt taagtatttt aggaatattc gtatatactt
    13561 ccagatactt agaatttaat gtttttatct caattgaaaa tgtaaattgt tctgtgcttt
    13621 tttctataaa tgaataccct gtcatgcttt ccatttattc cacccactat atctctttat
    13681 cttcacgtcc cagggactta attttcaatt ttccggtttc aaggaagaaa tcaactgttc
    13741 tgccataatc cccaaaaaca tcctgactga taatagttat acgatagtta tccaatattt
    13801 ctcttacttt ctggacattg ttaataccta ttgaacctat gaatgagttt tcaggcattt
    13861 taaacattga agcaccgcca gcaattttgg caaccagatg ctcaatattt gcaccttttt
    13921 ttcgcaattc atcaatcata agagctatcg cactatcagc atattttttt ggatttgttg
    13981 atttatcatt gttttccggt aacattatat gagccagccc gccaattcta ctttctggat
    14041 catacaggca aattccaaca caggatccca atattgtacg taatatatca ggagattgcg
    14101 atattttata atctgctatg ggaacagtaa taagtttaaa ttccatttac tttctccttg
    14161 ggaacatata caggcgaatc cataatcttg acatactgga aatcatcaga cagggcatgc
    14221 agagtttcag aatgaccaat aaacaggtac ccccatggat tcaggacctt ataaaaatgt
    14281 gctataagct ctttttgacg agcacggtca aaatatatca ttacattgcg gcaaaatatg
    14341 atatcaacat tttcaggata aggattttta aatagattga gatatgaaaa agaaaccagt
    14401 ttttttaact catcctttac ttcatacact tcaggattat caggcatttg tgtaaagtat
    14461 ttttttaaaa tttcaggtgg taccttttca attcgtcttc cagtgtacct gccaattttt
    14521 gcaaactcaa gcacagatgg agcaatgtct gttgctatga tggaaatatt ccatcccaaa
    14581 aggattgaac gtgcctccat aacacagata gctatagtgt aaggctcttc gcccgtggaa
    14641 caccctgcac tccatattct aagattctta tcctttttct tttttacaat ctccggtatg
    14701 catacatctt ttaaggcatc aaactgccgt tcatttctaa aaaagtatgt ctcattggta
    14761 gatacgacat caagcaactc ttcaatctct ttgcttttat ccttcaggct ggttatataa
    14821 ttataataat ctgaaaattc tgaaaggttt aatgccttta accgttttcg caaccggttt
    14881 gataaaagtg taatttttga ttcttttaaa aaaataccac tttcgtcata tattaattgt
    14941 gcaaattttt taaactcatc ttcatttaat ctcttgatat ttgaaaaatc aatcatactc
    15001 caccactatg caaatctttt tttataatta ctttttcatt gaaagattta atataagtga
    15061 aatttcgcca attggtatac cggatatttt cgatagcacc tgtaaaggat atccctgact
    15121 gtataatgta gctatcatta aatatttatc atcagcatta ttatacaaag ttatcagttc
    15181 ctcttcatca gcaggaataa ctacatccgc taatttttca tcacatttcg cttgagaagt
    15241 atcatattcc tcgataacac tatgtgatac atcttctgga tgtacaggaa tattaatgtt
    15301 tgctttttta gtatatctgg attcaatata ttgactgaat ttatgtaaaa ctaccctggc
    15361 tatctccgat aattgttgtg taattgttgg cacctgtgat ttattttcag aaaattgttg
    15421 ggttttttct ctttccagat atactatcgc attgtcatgt aattctttac cggttttttt
    15481 tgaaatatca aacaaaggat cagttaaaat tgtttgtata tcttctttgt cttgtatatg
    15541 ttctgcaaaa atattttctt cattcctgtt tattttcttt ctgctttttt ttgatccggg
    15601 ctttatttgg ctttcatcat taatggtaat gtcaagtcct ggcaatgcat ccgctctttt
    15661 taataattcc cgtaaaatat taattttgta ttcaagcaaa gctaaattac gttcagcagt
    15721 atggttaaat tcatttataa gatcttccat ttctttgcgc aaccgttttg accggaattc
    15781 agttgcagaa cgttctaatt ttaatgaaat aataagataa aatatagatg ctattaaaat
    15841 attcaccatt atgagaataa caagttgcat tgcgcatact tccttttttt atttttttac
    15901 gtcaataatt cttccgattt ttgtatcttc caatacttca ggttgttcct gtttttcctt
    15961 tttttcacct tctttaaatt gtttcccctt tttaaactgc ttttcttcgt ttaagataat
    16021 agttcgttct gcttttttat tttcttcaac cttctccttc tgtatattgg tcttttcctg
    16081 tgattctttg ctcaatatat gttgctgctc aactaatgca gcattacgcg catgctctcc
    16141 tcgtgcaacc tctatgatct gtgctatgtt agtttgcaaa tctattggtt taatagccat
    16201 tatattctcc tggtaataat gtaattatct tctcctcctt atcattaatt tttgagattc
    16261 ttccagatca ggtggttcat actcggatat cctgatatcg gctccctcta aagcaaattt
    16321 aacataattg taaggatctt tcacgttaaa ccgtttatct ttgatataaa tttccacacc
    16381 agggaatatg gttttttcaa cacatacttt tcctttttgt tccaacatat taagatatgc
    16441 tttcaattct tccaatgaca ggttaatttc attaagccgc tgttctaatt tttgcttctg
    16501 accaattagc tttcctaaaa gctcttccct gtcttttggc aaatgtcctc ctgcattatt
    16561 tttctgtact gtaagtgttg ttacatcttt cctgaccttg tcaaattctt ctgatacaac
    16621 cctcttgttc tcttccagtt cggttagctg ttgaagtact tttggattca tgccaacacg
    16681 gacaattgtt tttgtaaatg agtcagatcc aacatatttg gaatttactt cttcaccagc
    16741 tctgattaaa cccccaacga tacgtgctct tcttccattg cagatgatcc tgttacctgc
    16801 atcaacattt gaatgtacaa ttccctctgc aacaataaca tctttttcag caaccactgt
    16861 tgctgtctgt ataaatttag caatgataga accaccggtt gactcaattc ttgcgccttc
    16921 tcttcccata attccctgac ggataatgat atcaccttcc gcttcaagat tagctttctg
    16981 caccgtacct ttaatctcaa tattgcctgc agcttttaca gtaaaattgt cctgcacact
    17041 gccacttaca ataactgaac ccagaaatac aacatcacct gtttctgttg aaacatctcc
    17101 tttaactata aatacaggct caacactgag cttcccattt tgatagacaa cctgcccatt
    17161 tatttcagct gttaattcag taccatcctg tgacaggata gtacctttcc catacctgat
    17221 ctgtatatct ttaccggaac gagcagggat aatcctgttg gtaaccgttc ttcccggaac
    17281 tccttcctca gcaggaactt ttactgccag cacttgccca acaacaacat tttcaatcaa
    17341 atccagatcc ttaaagtcaa ccctcccttt ttcatcctct tctaattgaa tcccactttt
    17401 atcaattctg actttatagt caatataggc atcacgtcca tgcctgggtg gcgtaccctc
    17461 tgcagcaagg agtggctggc tataatccat tctatctaaa tactctgcaa ttctttcttc
    17521 tttaataccg gtaacaacac ccgcacgctt taatgcttca ataacatcat caacatccat
    17581 atgtcgtccg caaaagcggg gaggtacaaa atgtacaaaa gccttcattt catcatcagt
    17641 aatctcaaca gtcattgtac catcatattc ggggttgggg atccaatcac ctattttaac
    17701 aggtttacca gaaggttttc ggacctcttt ttcaacctta gtcatatctg cattttgtat
    17761 acgtaaagca taaattttat tggaaatatc agataagtca acacgcctgc catttccttt
    17821 tggtggtgtt attgtcagcc agatccctgt ttttgttacc cgtattttcg ctgaaccatc
    17881 aacatctttt ggaacatctt ttattctgag gtcatcttca tgaatggaat gaagtttttg
    17941 ttctatctct gcaaaatctt cctcttcttc tttctcagta attattggtc gcaccagtac
    18001 ccgataaggt tgtcttccaa tacccagaaa acctgatgtg cctttttcaa gaatctgata
    18061 atcaagttct gaaatgtcaa cacccagttc accagcagct aattctaatg cctgtttcac
    18121 tgaatcagca tacacttcta tttcattttc ggatgattca tcatcctgaa tagttttcat
    18181 taaatctttt aaatttttca ttatatcact aaccagtaat acattttaaa accgtaataa
    18241 atccatcttc tattttatcc aactatatta gatttaatcc tgccaagcct acctctcaac
    18301 ctcataattg cctttgtatg caactgtgat actctgcttt cagtaacttc taatacttca
    18361 cctatttctt ttaatgttag atcttcataa taataaagaa ctataacctt cttttcttta
    18421 tccggcaatt tctttattgc atctactata taatcccgta tttcttcttt ttcaatcatt
    18481 acatctggat tcagtgtatc cggagcttcc agagtttcaa gtattgacag ctcatcattt
    18541 tcatctccaa cataccatat atcattcaat gataacatag cagtaccact gagcttatta
    18601 agcagcgatt gaaattcttc aacagtgata cctaattcct ttgctatctc ttcatcttca
    18661 actgttcttc ctaacttatt ttcaaggtct gcaattgcct gttctatctg tttggctttc
    18721 tgccttattg aacgcggtat ccagtctata gaacgaagtt catcaaaaat tgcacctcga
    18781 attcttgtca tcgcatatgt tttaaattta ataccccgtt ccggatcaaa ttttgttatt
    18841 gcatcaatca atccaaatac accataagaa acaagatcat caaattctat tgaactgggc
    18901 attcccatag caattttgcc tgcaacatac ttaaccaacg gagcatactt cacaacaaaa
    18961 tagtctctta ttgcctgatc atgtgttttg gcaaagcgct tccagagttc atcttcatca
    19021 attgcatcaa attcctgaaa ttttttattt gccatacaac ctcgttattt taattaatcc
    19081 ttatcacgtt tgagcatagt tcgtatagct tctgccatta tttttggttc atattgaatt
    19141 ttcttatcgt ccagtacaat atgtttgccc atctttttgg gtttactatc cttagtactc
    19201 aaattagtaa aatcttttgc ttcaagtggt gtaaaaactt cattatatga tgaatcagat
    19261 gttcccgata ctgatgtctc tgcagtatac tcctcatcac gtgtagcact ttcttcccga
    19321 acttcgctgg tttcatccgg tttcacggaa acatattctt cctggcttac atttttttgt
    19381 gaaaattcaa aagattgatt taatatctca taaacctcag gcaccagacg ttttattatt
    19441 aaaagtgaac catatccaat tgctgcatat ataactgtaa aaaaaagtac cctgctaaat
    19501 aacatgctaa ttttattacc tgcaataatg ccaaataaca acgaaacaaa aaaagcaagg
    19561 ataccaaaga ttatggatac cttcccttct aatgaaatcc tgaacatttt ttcattcaat
    19621 aacattgtat aatgcttatc cttaaatttt ccttttaaat aaatccctac tatataaaaa
    19681 atagtgccag gtttaaatta aatcaagtaa taattacaac acattgacaa tagtatctat
    19741 tgtaatgaat cctcctcatt ttcagactca taatgtccaa aaaatcttct aaaaaagctt
    19801 agcatacctg tgcctttatc agtatccgat tcaatatttg caaccctgtc agctataatt
    19861 tctatacagg ttgaagcttt tgatttagga tagctcacta taaagggctt ctgattccgt
    19921 attgactttg caacatacat gtcatcaaat ataaacccta aattatcaac tttaatattt
    19981 aaaaactgtc cagcaatatt aatgaccctc tgagctactt tttttgcctc agttacagtc
    20041 tgtgccctat tcaccagaag cttcactgtt ttatctggat cattagctgc aatagcttta
    20101 atagttccat atgcatctgt aatagccgtt ggttcagggg tagtaactac tatcacttca
    20161 tcagaagcaa ccagaaaact cagcacattc tgtgaaatac cggcacctgt atcaataatc
    20221 atataatcat cgtcattcag ttccgatagt gccttgataa aatattcccg ttgcttaaca
    20281 tccagatttg caagttgatg aaacccggaa gcacccgcaa ttatcttaat tccttcagga
    20341 acatcaataa ttatctcctc caattttttc ttccctttta tgacatggta aagattgtat
    20401 tttggtatta tacccaatac aacattaaca ttggcaagac ccaggtcagc atctaacaat
    20461 gtaacagaat aattgcgttt agcaagtgct atagcaaggc taacagctac acttgttttg
    20521 ccaactccac ctttcccact ggtaatagct atagttttag ttctgcgagc agcattgttt
    20581 tgtattacca accgccttaa atttgcagcc tgatccaagg taccctcctt aacgcattgt
    20641 tgactccagt atcatattca ccatcatatc aggttcagca attgctatat catttggaac
    20701 ttcctgtcca ttagtgatat atgatatggg cttattatat ttatcggcta ctgagatgac
    20761 atttccaata tatgatgttt catcaacttt tgttattatt acattgtcaa tatgcaggat
    20821 gtcatatgct ttgaatacct cagatagatc actgcgtttt gtgttagcac taacacaaag
    20881 tatctttaca atctcacagg tacagttatc agcaaagttt tttatctcgg caattttaag
    20941 atcattcctg tggcttcttc ccgatgtatc taccattata atgtcagctt tttcattttc
    21001 aactatttct ttaaattcag ccggatcagt aacaacatga attggtatgc gcattatttc
    21061 agcatatttt ttaagctgct cagttgcggc aattcggtaa ttatcaatag taataaatga
    21121 tactttcaaa ccctgccgca aagataatat agcacccaat tttgccatcg ttgttgtttt
    21181 accaacgccc gttggaccta caaacatcaa cactttcttc cgtttgcccc ggtcaatagg
    21241 tccacttacc actatcctgc ttttaatgag ttcacgcaac gttttttcaa ttttatattt
    21301 atctttcata tcttcactgc ttacattgcg ttttacttca tctaaaagca tagtgcattc
    21361 ttcatcatcc atatcatttt tacgcaaaat ttccttaaac ggtttaaaat atgtatcacc
    21421 gttatcaaga catatatccg aagacagtct ttcttgcttt gtctcaacaa gatttttaag
    21481 taaagctttt aattcgttaa attccttctc caattttatg tatgaagaat cggttgtttc
    21541 aactttctgt tcctcaaccg tcttcactac aggcttaacc tcttcctgct gggttcttga
    21601 ttgcagtata ctctgagtta aaagttgctc aagtcctgta tgtggttttt tatgtggttc
    21661 agtttcaggt ttttctataa cctgttttaa aagctcattt acattatcat tggttccaac
    21721 ggttaaatcg atgttcttct ttggttttaa ttttgcaggc ttgacatctg gtattgctgc
    21781 agtcaactca acaaaatcgc gtgcaaacaa tcctgtatta aacaaaccgc cttctttaac
    21841 attcttatgg ctgataggta tagcatcatc accatgttcc atcttgagtt tcatcattgc
    21901 ttcattatag gtgcgtgctt taatttttac gtatctcatt gttaattacc tcgctttaac
    21961 aaatatttta ttatattatg ctgcccgcaa taacgctgta cctataacat tcactctgat
    22021 ctcgggcaca atttcattat acgaaagaac aacgggatca gggacctccc tttccagcaa
    22081 tgtaaacaag acactacgaa tatgtggcga taccagatac accggtgcaa atcctgcact
    22141 tcgtgccttg gaatatgaac ccagcaaact ctccagtacc tctctatgcg tatctggatc
    22201 aaggctaacg attcttccct caactggatc ctcgtgtatt gaagatcgta ttattgtttc
    22261 tatctccgga tctactgtaa ttaccgaaag cgtattgttt ttatcagcat attccctgca
    22321 tatctgacgt gccagtgcaa c
//
LOCUS       DAORYL010000050        22295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1968_length_22295_cov_12.854002, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000050 DAORYL010000000
VERSION     DAORYL010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 22295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22295
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1968_length_22295_cov_12.854002"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..714
                     /locus_tag="PLV81_09830"
     CDS             73..714
                     /locus_tag="PLV81_09830"
                     /inference="COORDINATES: protein motif:HMM:NF028235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOM10434.1"
                     /translation="MVSKGTVTKQTIINRIIEHIHLNGFSETSLSDIIAITGVKKGNL
                     YFHFKNKEELILEALKEAHRQYQEYLAAYTAKVSEPLHKIDAMLDAVYTYHKKRKFRG
                     GCIFGNIALEMADKNIAYRGFIHDVFQQWIDWTATQLEAAVAGGQLLASTPVQELSHH
                     IIAALEGGLLLSKVTKNPDDLNAAIRGIKELLHYYKTNKGLKRRNNAIKTGNN"
     gene            689..1798
                     /locus_tag="PLV81_09835"
     CDS             689..1798
                     /locus_tag="PLV81_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HOM10435.1"
                     /translation="MLSKQEIIDKARELQFDDIGFATAEPFEEHKQFLTTVPDNFTWA
                     RVFGLPLEEGCNPQSILPQAKTIIVLIENYFKFSFPKSMEPYFGRCYMDDDRVTKDGL
                     TVRIRQFVSFLREHGLQAKVTFSIPQKAASARAGIGNFGKNCLLYANNAAGKSSWISP
                     ITILVDKAFEADEPTVKVGCPDWCRNACVAACPTRALKGNCTIDSKRCISFLTYFGQG
                     ITPRELREPMGTYIYGCDRCQDVCPRNRAWMSQVLPLNPRVAAKEPWFDIVKLLHMDD
                     AYFQHNIQPHMFYMPASDLWRWKMNVARAMGNSLNERFVPELIKAYEDNSDDRVRGMI
                     AWSLGKIGGAKAKAALERWRDSATPAVKEEIEMAL"
     gene            2225..2605
                     /locus_tag="PLV81_09840"
     CDS             2225..2605
                     /locus_tag="PLV81_09840"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HOM10436.1"
                     /translation="MDTINPTQEQIRLIKDKYFEKPFVMINLLKFKRDSSGRKSKQLY
                     HQYTKEVASLLQAIGGNVIFLGAVHDVFIGEQGNMWDEVLLVYYPSGEAFLRMIAMEE
                     YQKANAFREAALEKCVLMVTQRLV"
     gene            complement(2632..3300)
                     /locus_tag="PLV81_09845"
     CDS             complement(2632..3300)
                     /locus_tag="PLV81_09845"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOM10437.1"
                     /translation="MTKEVSAIIQNAQSHGWVMEHQARQIFLYYGLPVSRYAYAESIE
                     AALSAAKNVRYPLVAKVVSPEVIHKSDVGGVVVGISNDNQLKEVFLKFTMIKGFKGIL
                     LDSLQSGVEMIVGSKRDPQFGPVVLVGIGGTSVEIYKDVAIRMAPMTRNEALKAIESL
                     KGVQLLKGFRGHQEAHISALAALLAKFSTMVIDLADAIKSIDCNPVMVNTKRAVIADA
                     RIIL"
     gene            complement(3297..4661)
                     /locus_tag="PLV81_09850"
     CDS             complement(3297..4661)
                     /locus_tag="PLV81_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOM10438.1"
                     /translation="MNLKQVIEPKSIAVIGVSLSNPFNPANVIFTKNRLRYQARTYCI
                     NPKGGKLYGDTVYTSISEIPEKVDLAIFSIRADMIPRSMEECIAAGVSGGIIISGGFS
                     ESGRQDLQEEIVRIATTHSFPVLGPNCLGIFSPPHIDTFFLPNERLIDLKKGNVALIS
                     QSGGILVDLTIRLTQEGVGISRAVSIGNKAVLDEVDLLQFFIKDPATQVIGFYIEGFS
                     NGRGRLFIEEVNHTKKPVVIMKSGKTPDGSKAVSSHTASLSGDYITFSEIIKGSRAVE
                     AADEAEFAAFCEVLSRYSASNVKNVCIITASGGHGAIASDGCYSAGITTVDIPKNDQE
                     ELKNLVSKSIQGIASFSNPIDLTGSAVDDDFITTTRYFIGKEYVDCVLILLLPYLPGI
                     TSDVGARIAQIARESGKPVVTYIPHVDKYGIFIDGFESNGIPVAHSVEGAVSMVRAIA
                     GGKL"
     gene            5039..6082
                     /locus_tag="PLV81_09855"
     CDS             5039..6082
                     /locus_tag="PLV81_09855"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF004127.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOM10439.1"
                     /translation="MSNIIIRNSPASLSIILNRPEAINSLTVEMIDCITSALKQALHN
                     ESVHFILIYAYGSKGFCAGGDIKKLYQASVSGDSNYIKEFYSKEYALDYLLHTYPKPV
                     IVFAHGITMGGGMGIAAGATRVVVTEQTMMAMPEGRIGFIPDVGSTGWLFTKCKKGYP
                     YYLALTGDTLYGAECVHAGLAHNQILSHIVSQCIAELERLQCSNDEVLSRINSILSFY
                     STPVQLSQGAKDEWIGQYFDTDKPVQCMLQSLKQCSTHHKLCNGVFTRIAERSPTSLV
                     LTHLLLKVNKGKPLDEVFARELKAAIFMTQHNDYREGVRARLIDKDNKPEWQPEKIED
                     VDSALLQKVISSN"
     gene            complement(6079..7206)
                     /locus_tag="PLV81_09860"
     CDS             complement(6079..7206)
                     /locus_tag="PLV81_09860"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOM10440.1"
                     /translation="MSIPRPNHSDTLDTVARYIQDILAQHNIPFELQHVTIYPYIMLI
                     AGLLCFILALLFAWCIKKQKTLPGLVFLLAIPFILICEFELVKPVVSWIVPKESANII
                     VSFPQHHATHTLVLVAHYDSKTDIFDHIERAKIYQFIVPSILAGLLVLLWLALSKKIT
                     LLTKPLYRRIAAIIVLLCIIEWLLVAVAMGGFIFLKQQSYGAIDNASSVVALLGLADN
                     IKQGTVVNKSLNVEIVFTTGEEINLQGAKWYVKKLLSNKKPQELSVINLELVGEPGNM
                     VYWEKAGVMLTFYSADSNLIQRLAKAYRTVTKKDVGTLPKLTDDSIMFMREGIPAITV
                     GFANETYGVSGLHSTKDNLQRVSMDNVHTMVKTLGEIITHF"
     gene            complement(7248..>7863)
                     /locus_tag="PLV81_09865"
     CDS             complement(7248..>7863)
                     /locus_tag="PLV81_09865"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOM10441.1"
                     /translation="WDLPSPLTYCSLSKNKLIQTFIEGLGDSYFKEEAFNKLTAECSS
                     KELMNIAIAIEKKYAKLNSYKEMEYAPYRDRWQTRFWLAAQYGKENNNGNLPTAIYTK
                     NNSEAKKLMDQVLDCTFRDANGTTLLMIAAYTDNVEIASLLLAKKCNINARDNHGCTP
                     LMYAVFGNAAHTMELLLKNNANVMMESPLLLKLKNYIIVALLNN"
     assembly_gap    7864..7963
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7964..9733)
                     /gene="ade"
                     /locus_tag="PLV81_09870"
     CDS             complement(<7964..9733)
                     /gene="ade"
                     /locus_tag="PLV81_09870"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOM10442.1"
                     /translation="MDFIPLENITMELADVAMGRRKADMVIKNGTLVNVNTKELQEHI
                     DVALYKGRIALVGDAKHCIGESTEIVDATGLFIAPGFMDGHIHVESSMLTVSQYSNAV
                     VPHGTTAIFMDPHEIANVLGLKGVRLMLDEAKDVPLRVYATVPSCVPSAPGLEDAGAV
                     IGASDIEQAMQWEGIAGLGELMNFPGVFNSVPEVHKEIEVTLKAGKPVTGHFALPDTG
                     AMLNAYVASGVRSCHESVRAEDALAKMRLGMYAKIREGSAWHDLKEVIKAITQNTVNS
                     RFAVLVSDDTHPHTLIELGHLDHIVKRAIEEGVDPVTAIEMVTLNTAECFMMSKDFGS
                     VSPSKVADIVLLSDLYKVTVKAVIIGGKLVAKDGTILIPAKKVTYPDWSKNTVNVGKT
                     LTKDDFILKSNKTQAKVRVIKIQEASVLTQQVFEILPVVNGNIAPDPTKDIAKAALFE
                     RHKATGTKGIGFVTGFGFITGAVASTVAHDSHNLLIVGTNEDDMAFAGNELIKAGGGM
                     IAVANGKVLALVELPVAGLLTDEPVDVVQKKVANLEKAWKELGCTLVSPFMTMALLGL
                     PVIPELRLSNRGLVDAIHFTFVDV"
     gene            complement(9788..11182)
                     /locus_tag="PLV81_09875"
     CDS             complement(9788..11182)
                     /locus_tag="PLV81_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258243.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOM10443.1"
                     /translation="MKKTSTLGDFFLGGRTIGPWVSALAYGTSYFSAVLFIGFAGKLG
                     WVFGLDAIWIGVGNALFGSFIAWLVLGRRTRIMTQNLDVMTMPEFLMERFNGTYIKMF
                     AAIIIFIFLLPYSASVFKGLGHLFEVNFHISYDLALIIMIGITGIYLVLGGYFAITLT
                     DFIQGIIMIAGAITMVLVLSAKSGGIAQSIALVAQKYPLHIPADKHPGYLLLGALIFM
                     TSFGVWGMPQMVQKFYAIKSEDLIKKASIVTGIFATIIGVSAYYTGSLSHIFYDAVPA
                     IDGKPAFDRLIPDLLTQNLPEPLMAVILLLILSASMSTLSSLVLVSSSAIAIDLYKGH
                     VNPQISKENSVAMMRFLSALFIIISYFIARYEFAIIITLMSLSWGAVAGAFMAPYLYG
                     LFWKRTTLAGVYAGMITGLVTSIGLFFYLGANLSPIASSAGMLAPFVVVPLVSLFTKK
                     PSDETITKAFEKIG"
     gene            complement(11430..>12728)
                     /locus_tag="PLV81_09880"
     CDS             complement(11430..>12728)
                     /locus_tag="PLV81_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOM10444.1"
                     /translation="EKEHGADIYSTDMMLYPKGDGTYLARGDKYYIGNGNAAALISVF
                     GKMADTGEYVFFVVDTKHPNYECVKKISTSGVRQAYVAEFALHDYPITEADILSRGNH
                     AWDSSLNTVNVGKYELGWASIGICEHAFYEAIDHAANRNLYNMYVTDFPHVKKIFTEA
                     FARLAAMKLFALRACDYMRSANENDRRYLLYNPIQKMKVTMEGEKVIGLLHEVIAARG
                     FEQETYFEMAIRDIGMLPKLEGTTHVNMALVVKFLQNFMFKPKDYPEVPRRDDPSNDS
                     FLFNQGPTKGLGSIQFHDYRKAYEGIRSKNLEVFMEQIEAFKNFLIKGTPDEKQTKNI
                     DYMLTVGELFTLIPYTQLICENKKIYNVEDEIIDEIFKFIVSDFSAYATRLYTGQKNT
                     DKQNELILKMIKKPVLDDENFNKVWKDHVYALKGQYTMNE"
     assembly_gap    12729..12828
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13334..13567
                     /locus_tag="PLV81_09885"
     CDS             13334..13567
                     /locus_tag="PLV81_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOM10445.1"
                     /translation="MTFYEIGEEIKRIRKQKKITQGILCKTGISRPTLSKIEQGEFVN
                     VSVRALDKILVALGYELSLQPRNIVGLPPLQDD"
     gene            13615..14784
                     /locus_tag="PLV81_09890"
     CDS             13615..14784
                     /locus_tag="PLV81_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015024465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM10446.1"
                     /translation="MIKRKIEKTLKELAKQYPVVTITGPRQSGKTTLCRYVFKHKNYV
                     NLEAPDIRMFALEDPRGFLKQHGNNVIIDEIQRVPQLISYIQTIVDDNPESGQFILTG
                     SSHFGIMNGISQSLAGRTAIITLLPFSYEEIQHIVPCNIDRLLYTGFYPRIHDKKLNP
                     TQALADYVATYVERDLRQLAAIKDLYTFEKFLRLCAGRVGQLLNMQSLASDVGVSHTT
                     IRAWISILEASYIIFLLQPWYGNVAKRLIKSPKLYFYDVGLATYLLGIENEKQMQRDP
                     LRGNLFENMVIAEIIKYRFNRGKRSNIFFYRDSKGTEVDVLYQYGRNIFPIEIKAGAT
                     VVDDFFKNFNAVCKYIDCEKGAVIYGGTEIQKRTGVDVIPFVKIPDLLQQIDKNQ"
     gene            14981..15994
                     /gene="gmd"
                     /locus_tag="PLV81_09895"
     CDS             14981..15994
                     /gene="gmd"
                     /locus_tag="PLV81_09895"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOM10447.1"
                     /translation="MKRALITGITGQDGAYLAELLLQKGYTVIGGFRRLSTPNFWRLE
                     ELNILDKIVMVEIDMLDAGNLVRVIEKYKPDEVYNLAAQSFVAHSFANPVLTGNVTGL
                     GVTNILEAIRIVNKDIKFYQASTSELFGKAQETPQKETTPFYPRSPYAVAKLYGHWMT
                     INYREAYGIFASTGILFNHESPLRGIEFVTRKITDAIAKIYHEKQDYFEIGNLDAKRD
                     WGYAKEFVEGMWRILQAPRPDNYVLATGETHSVREWVEVCFNYIGKNIVWDGTGVDEV
                     GKDAKSGKTLIKVNPEFFRPAEVDYLVGDASKAKKELGWEPKVKFKELAEIMLKRDIE
                     RNR"
     gene            16262..16570
                     /locus_tag="PLV81_09900"
     CDS             16262..16570
                     /locus_tag="PLV81_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10448.1"
                     /translation="MNTFERIKKECFWDYKITPEEIKIIAESGTKEEKKKLFQKIMYN
                     SRDKLGHLMIFKKDELREFFNDFVPAYNTMYLRKHMKVLKYLLLKEHEPVQELAWKEK
                     "
     gene            16555..17226
                     /locus_tag="PLV81_09905"
     CDS             16555..17226
                     /locus_tag="PLV81_09905"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOM10449.1"
                     /translation="MEREIDYTALYALQDDVLKVIFNLENNFYITGGTALHRFYYNAR
                     YSDDIDLFVNNSFTFHEEVQEIFEAIANNGFDIERSVSTRDFYRIMVDNILRLDFVND
                     RVYRHGKSTIINSIRVDNKINILTNKITAIINRDEEKDIFDLFCFAYNENFNWEEVLH
                     IANKKHPVEKDILVYRLKTFPLNWLKRIKTIKSVSITPEKIAILCDDIINNTQNSLVG
                     SSFNQ"
     gene            17647..18807
                     /locus_tag="PLV81_09910"
     CDS             17647..18807
                     /locus_tag="PLV81_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005770051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM10450.1"
                     /translation="MIFVQRFILPPPTSFFLFGPRGTGKSLWVKNTFVNALYIDLLDP
                     EYFRVYTAYPERLKELLNAQPQKKDIIIDEIQKVPYLLPLVHSLIEEKKGYRFILTGS
                     SSRKLKRSGVDLLAGRALHYTMHPFLPSELGEHFIFEKALQYGLVPLVYFSDSPEQTL
                     AAYVSLYLKEEIQAEALIRNIPAFTRFLEALSFSHASVLNVSQVARECMVERKTVEGY
                     LKVLEDLLLCFTIGVFSKRAKRKLISHSKFYFFDTGVFRHLRPKGPLDRLSEIDGQSL
                     EGLVAQTLRTWMHYRNKNNTLYYWRTINGLEVDFIVYGEDGLYAIEVKNARSIYPVDL
                     KGLEAFKKDYPEAQCILVYRGNERLQKGDVVCIPVEEFLYKLHPDRPIMNIL"
     gene            19056..20186
                     /locus_tag="PLV81_09915"
     CDS             19056..20186
                     /locus_tag="PLV81_09915"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOM10451.1"
                     /translation="METAFYIYIFVFPFIISTVLIPVIIQFSHKNGLYDKVDARKVHK
                     GKISRLGGLAIFAGFVSGFICLLIYDFQVQFNIVVFIIALLLAFFTGFIDDVITIRAM
                     YKLLLQLVCGLLVALSGLKITKVTLFAGFDVQFGILSYLFTMVWVAGFMNAINMLDGM
                     DGLASGIVLVASMFLGIMGWLQGIPIVTYLCVVLIGAILGFLLYNYPPAKIFMGDGGA
                     YFLGFIYAVLPLIGIKKASTITIFSIPIILLLIPLIDVINVMRKRINAGYHIFIADRN
                     HIHHRLLKIGFSNEGILWVMYSYTVILGGFAVLMLYLSPLHAIILTVFLFMLTILTFY
                     IISVAEKRIEELEGRSVKQQKTQKIKTLQKVKNSIIYKKLFT"
     gene            20348..21061
                     /locus_tag="PLV81_09920"
     CDS             20348..21061
                     /locus_tag="PLV81_09920"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOM10452.1"
                     /translation="MEERNVIYDDEIDLYELVVKLWKYKTFIILFTIFCTVASLVAVN
                     FITPRYKVTCQVVPNNDEFVKLFLREINKYNDPKLWLSDIRFNYYQEIIAPLFQSRNL
                     LMRAIKDALPGDFDHAFWMEVLDEQLLDVKVERDKQLNNVITIGIEYTNAELVYKIAK
                     NLLVKVEDELKTMYAINNSGKQLLIVTQTPTLPNKPCSPKKTLIIAVTFVTSLFFAVF
                     MALIVDWIRTARKQYLREQ"
     gene            21608..21847
                     /gene="vapB"
                     /locus_tag="PLV81_09925"
     CDS             21608..21847
                     /gene="vapB"
                     /locus_tag="PLV81_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009546660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HOM10453.1"
                     /translation="MLKIAKVFKNGRSQAIRIPKEFKITSKEVYIEQEGNKIIIYPKY
                     DGIWDAFFQKLKEVDTNGFLEERNQLPLQKRDILL"
     gene            21844..22242
                     /locus_tag="PLV81_09930"
     CDS             21844..22242
                     /locus_tag="PLV81_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOM10454.1"
                     /translation="MIYMLDTNVCSYIIREKPYHLKDKIVKVYKEHTVSLSSIVVAEL
                     LYGAKKKNSKALTQIIELFIENFVIFDFDRKAAVEYASIRDSLEKSGNIIGSNDLFIA
                     AHAKSLNAVLVTNNAKEFKRIKGLKVENWM"
BASE COUNT         7145 a         3909 c         4297 g         6744 t
ORIGIN      
        1 aacatataat aatcattgac aaatttgttt tatcaaaata atgtaccaac tggtcagtac
       61 aggatggtat atatggtatc aaaaggaaca gtaacaaaac agacaatcat taaccgcatc
      121 attgagcata tacacctcaa tggtttttct gaaacaagtc tcagtgatat tattgccatt
      181 actggcgtta aaaaaggtaa tctatatttt cattttaaaa ataaagaaga actaatttta
      241 gaagcactca aagaagctca tcggcagtat caggaatatc ttgcagcata tactgcaaag
      301 gtttctgagc ctctacacaa aatagatgca atgcttgatg cagtatacac ataccacaaa
      361 aaacgtaaat ttagaggcgg gtgcatcttt gggaatatcg cactggaaat ggctgataaa
      421 aatatagcct atcgaggatt catccatgat gtttttcagc aatggattga ctggactgca
      481 acacagctgg aggctgctgt ggcaggtggg caattgttgg ccagtacacc ggtacaggaa
      541 ctatcgcacc atattattgc tgcacttgag ggagggctcc ttctatcaaa agttacaaaa
      601 aatcctgatg acctgaatgc tgctattcgg ggtataaaag agttattaca ttactataaa
      661 accaataaag gtttaaaacg gaggaataat gctatcaaaa caggaaataa ttgataaggc
      721 aagagagctg caatttgatg atattgggtt tgcaacagca gagccatttg aagaacacaa
      781 acagttttta acaacagtac ctgacaattt tacatgggca cgtgtctttg gtctgcctct
      841 tgaagaaggc tgtaatcctc aaagcatact tccgcaggca aagactatta ttgtgctcat
      901 tgaaaactat tttaaatttt cgtttcctaa atcaatggaa ccatactttg gacgttgcta
      961 tatggatgat gaccgtgtaa ccaaggatgg gttgacggtc agaatacggc agtttgtatc
     1021 ctttttgcgt gaacatggtc ttcaggcaaa agtgacgttt tccattccac aaaaggctgc
     1081 ttcagccagg gcaggcattg gaaattttgg aaaaaactgt ttactgtatg caaacaatgc
     1141 agcgggcaag agttcatgga taagccccat caccatactg gttgataaag catttgaagc
     1201 agatgaacca acagtaaaag taggatgtcc tgattggtgt cgtaatgcct gtgtagctgc
     1261 atgtcccaca cgagcgttga agggcaactg tactattgac tccaagcggt gtatttcatt
     1321 tcttacatat tttgggcagg gtattacgcc tcgagaactt cgagaaccca tgggcacgta
     1381 tatatatggt tgtgatcggt gtcaggatgt atgtccacgc aacagagcat ggatgtcaca
     1441 ggtgctgcca ctaaacccgc gcgtggctgc aaaggaacca tggtttgata ttgtaaagct
     1501 gcttcatatg gatgatgcct attttcaaca caatattcag ccacatatgt tttacatgcc
     1561 tgcaagcgat ttgtggcgat ggaagatgaa tgtagcacgg gcaatgggta atagtctcaa
     1621 tgagcgtttt gtaccagaac ttattaaagc ttatgaagac aacagcgatg atcgtgtgag
     1681 aggaatgatt gcctggtcac tgggaaaaat tggtggcgca aaagcaaagg ctgcacttga
     1741 acgctggcga gatagcgcta caccagcagt gaaagaagaa attgagatgg cgttgtagat
     1801 atatatcgta gtatagaaaa acttggaact tatttcattt ttaaattaac tatctatcta
     1861 ttgaaatcta tttaatattt tataaggaaa gattaattgt ggaaaaagag ataatattgt
     1921 atatagacga atcacttgag agtgaatatg aagcaagggc acctggttat tctataatta
     1981 aacaaatggg aagccatata cgttcgacaa caaacaaaaa tggcaatcat tacattaccc
     2041 tcccagcaca taaaacattt aaaataggta cattatgatg tgggatgctt cacgatattg
     2101 ctgaacattt ttatatttca aagacgaatt aatagatata ctctgggaat aatgttatga
     2161 ccatgatatt tattttaaaa tttctataca aatttatatt tataatctta aaaaggtaaa
     2221 acatatggat accattaatc caactcaaga acaaataaga ttaattaaag acaaatactt
     2281 tgaaaaacct tttgtcatga ttaatctttt aaaatttaaa agggatagtt ccggtagaaa
     2341 aagcaagcaa ctgtatcatc agtatacaaa agaggtagct tcattgttac aagcaattgg
     2401 tggtaacgtt atatttttag gtgcagtaca cgatgttttt ataggggagc agggcaatat
     2461 gtgggatgaa gttctgctgg tatattaccc atccggtgaa gcttttttaa gaatgattgc
     2521 catggaagaa tatcaaaagg caaatgcatt ccgtgaagcg gcacttgaga agtgtgtatt
     2581 gatggttacc caacgtctgg tgtagtatac cttgatttac ttcatatatt gttataaaat
     2641 tattcgagca tcggcaataa ccgctctttt tgtattaacc ataacagggt tgcaatcaat
     2701 tgatttaatc gcatcagcca aatcaatgac catggtggaa aattttgcca gaagcgccgc
     2761 cagggctgat atatgtgctt cctggtgccc gcgaaagccc ttcaacaatt gtaccccttt
     2821 aagcgattca attgctttca aagcttcatt ccttgtcata ggagccattc tgattgcaac
     2881 atctttataa atttcaaccg atgtaccacc aattcccaca agtaccacag ggccaaactg
     2941 tggatccctc ttagaaccca cgatcatttc tactccactt tgaagagaat caagaagtat
     3001 gcccttaaag ccttttatca ttgtaaattt taaaaaaact tctttcagtt gattatcgtt
     3061 agaaattcca acaacaactc caccaacatc agatttatgg attacttcag gggaaactac
     3121 cttggcaacc aaaggatacc taacgttttt tgctgctgaa agagctgcct caatactttc
     3181 agcataagca taccgtgaga caggaagacc atagtaaaga aatatttgtc ttgcctggtg
     3241 ttccattacc catccatgtg attgagcatt ctgtatgatt gcacttactt cttttgtcat
     3301 agtttccctc ctgctattgc cctgaccatt gaaacggcac cttcaacgga atgtgccaca
     3361 gggattccat ttgattcaaa tccatcaata aagattccat acttgtcaac gtgaggaata
     3421 tacgttacaa caggttttcc cgactcacgt gcaatttggg caattcttgc tccaacatct
     3481 gaagtaatcc cgggaagata cggaagcagc agaatgagca cgcagtcaac atattctttt
     3541 cctataaaat accgtgttgt agtgataaaa tcatcatcca cagcactacc agtaaggtca
     3601 attggatttg aaaaagaagc aattccctgt atgctttttg aaacaaggtt ttttaactct
     3661 tcctggtcat tttttggaat atcaactgtt gtgatgcctg ccgaataaca tccatcggaa
     3721 gctatagctc catgtccgcc gctggcagtt attatacata catttttcac attacttgca
     3781 gaatagcgtg ataaaacctc gcagaatgcc gcaaactcag cctcatcagc tgcttccact
     3841 gcacggctcc ctttaatgat ttccgaaaaa gtaatataat caccggacaa tgatgcagta
     3901 tgtgaactta ccgcttttga tccatctggg gttttaccag atttcatgat cacaacaggt
     3961 ttttttgtgt ggttgacctc ttcaataaaa agccgtccac ggccattact gaacccttca
     4021 atgtaaaaac caattacttg ggtagcagga tctttaataa aaaattgtaa caggtctact
     4081 tcatccagta cagctttatt gccaatacta actgcccgtg atatgcctac cccctcctgt
     4141 gtcaatctga tggtaagatc aacaagtatt ccgccacttt gactgataag ggcaacattt
     4201 ccttttttta aatctattag cctttcattg ggcaaaaaaa aggtatcaat atgaggtggg
     4261 gaaaatatcc caaggcagtt tggtccaaga acaggaaagg agtgcgttgt ggcaatacgg
     4321 acaatttctt cctgcaaatc ttgtcttcct gattcagaga agcctcctga tattattatt
     4381 ccaccagaaa cacctgcagc tatgcactct tccatggatc ggggaatcat gtctgctctt
     4441 atactaaaaa tggccaggtc caccttctcg gggatttcgc ttattgatgt atatacagtg
     4501 tctccataaa gcttcccacc tttaggattt atgcaatacg ttcgcgcttg atatcgcagc
     4561 ctgttttttg taaaaataac attagccgga ttaaaagggt tggaaagtga aacacctatg
     4621 actgctattg attttggttc aataacctgt ttaagattca tatctgctcc tttgtgtaag
     4681 gaattatatt atatagtatc atcgttcaat tatatatttt caataaaaaa atgaataacg
     4741 ccattctaaa ctcataacaa aaaatggcta ctatattgat actataaaaa acaatgaaat
     4801 tcaaacaaga caaaatcagg aatcgctttc aatcaatgaa cttgattgcc ttattgaatt
     4861 aagtcaaacc ctgattcatt tggaaataaa agcagtgtgt aaagataata ctgagtttaa
     4921 ttaaaacctt gaaaaaattt tccacatgca gtaatgcaaa aaaaggattt atgttgtact
     4981 caggcaagca agctgctggc attacatctg atatccctat aattttctgg tggatgctgt
     5041 gagtaatatt ataataagga atagccctgc atcgctttcc attattctta accgtcctga
     5101 agctattaac agtttaactg ttgaaatgat tgattgtata accagtgcat taaagcaggc
     5161 acttcataat gagtccgttc attttatttt aatctatgca tacggcagca aaggcttttg
     5221 cgctggcgga gatatcaaga agctctatca ggcttctgtt agtggcgata gtaactacat
     5281 aaaagagttt tattccaaag agtatgcgct ggattacctg ctgcacactt atcccaaacc
     5341 agtgattgta tttgcacatg gtattacgat gggtgggggc atgggaattg cagcaggtgc
     5401 aacgcgtgtt gtggtgacag agcaaaccat gatggcaatg cctgaagggc gcattggctt
     5461 tatccctgat gtggggtcaa cggggtggct ttttactaaa tgtaaaaaag gctatccata
     5521 ctatttagca ctcacaggcg atactctcta tggtgcagaa tgcgtccatg ctggcctggc
     5581 acataatcag atactatcgc acatagtatc acaatgtatt gctgagcttg aacgtttgca
     5641 gtgcagcaac gatgaggtac tttcaagaat taatagcata ctttcttttt atagtacacc
     5701 tgtacaactg agtcaagggg caaaagatga atggattgga cagtactttg acactgataa
     5761 gccagttcaa tgtatgttac aatcactgaa acaatgctcc acacaccaca agctgtgcaa
     5821 tggcgttttt acacgcattg ccgagcgttc ccccacatca cttgtcctga cccatctatt
     5881 gctgaaagta aacaaaggca aaccccttga tgaagttttt gcacgtgaac taaaagctgc
     5941 catttttatg acgcagcata atgattaccg tgaaggggtc agagccaggt taatagataa
     6001 agacaataaa ccagagtggc agcctgaaaa aattgaagat gttgatagtg cgttactaca
     6061 gaaggtaatt agcagtaatt aaaaatgagt aatgatctcg cctagcgtct ttaccattgt
     6121 gtggacatta tccattgaca cacgctgcag gttgtctttg gtgctgtgca ggccactcac
     6181 accatacgtc tcatttgcaa agccaacggt gatagcgggt ataccttcgc gcataaacat
     6241 gatggaatcg tcagtgagtt ttggtaatgt gccaacatct ttttttgtaa ccgtacggta
     6301 agcttttgcg agcctttgaa tcagattgct atcggcacta taaaatgtaa gcattactcc
     6361 ggctttttcc cagtacacca tgttaccagg ctccccaaca agttctaaat ttatgaccga
     6421 tagctcctgt ggctttttat tggataaaag ttttttaaca taccatttag ccccctgcag
     6481 attaatttct tcaccagttg tgaacacaat ctccacgtta agcgatttat tgaccacagt
     6541 accttgcttg atattatcag caagccctaa aagggcaacc acggaagaag cgttgtcgat
     6601 agctccgtat gattgctgtt ttaaaaatat aaatccaccc atagcaacag ctactaaaag
     6661 ccactcaatg atacataaca gcacaattat tgcagcaatg cgcctatata atggctttgt
     6721 taataatgta attttttttg ataaggccaa ccaaagcagc acaagtaatc ctgccagaat
     6781 gcttggaaca ataaactggt atattttggc tcgctctata tggtcaaata tatcagtttt
     6841 ggaatcatag tgtgcaacaa gaaccagcgt gtgtgttgcg tgatgttgtg gaaatgaaac
     6901 aattatattt gcactttctt ttgggacaat ccacgaaaca actggtttca caagttcaaa
     6961 ctcacagata aggataaacg gaatagctaa aagaaaaact aaaccaggca atgtcttttg
     7021 ttttttgata caccaggcaa aaagcaatgc aagaataaaa cacaaaagtc ctgcaatgag
     7081 catgatgtat gggtaaatag taacatgttg caactcaaat ggaatattat gctgtgcaag
     7141 tatatcctgt atgtaccggg caaccgtatc cagtgtgtcg ctgtggttgg gccttggtat
     7201 ggacatgtgt tttaaaattt catacaggtt cataggaata gtatgaatca attatttaat
     7261 aatgcaacaa taatataatt ttttaatttt aataataaag gtgactccat cataacattg
     7321 gcgttgtttt ttagcaataa ttccattgta tgtgctgcat taccaaacac ggcatacatc
     7381 agtggtgtac agccatgatt atcacgggca ttgatattac attttttggc aagtaacagt
     7441 gaagcaatct caacattatc tgtatatgca gcaatcataa gtagcgttgt accattagca
     7501 tccctgaatg tacaatctaa aacctggtcc atgagttttt tagcttcact attgtttttg
     7561 gtatagatgg ctgttggtaa attaccattg ttgttttctt ttccatattg tgctgccaac
     7621 cagaaacgag tttgccatct atctctgtag ggagcatatt ccatctcctt gtaactatta
     7681 agttttgcat attttttttc aatagcaatg gctatgttca taagctcttt tgaagagcac
     7741 tcagctgtta atttgttaaa tgcttcctct ttaaaataac tatcgcccag tccttctata
     7801 aatgtttgta taagcttatt ttttgatagt gagcaatagg taagtggtga aggcagatcc
     7861 catnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncacatcc acaaatgtaa
     7981 aatgaatggc atcaaccagg cctcgattac tcaacctgag ttcaggtatt acaggaagcc
     8041 ctaagagtgc catggtcata aacggtgaca ccaatgtgca tcctaattct ttccatgctt
     8101 tttctaagtt tgcaaccttt ttttgtacca catcaactgg ctcatcggtt aatagccctg
     8161 caacaggaag ttcaacaagt gcaagtactt taccatttgc aacagcgatc ataccgccac
     8221 cagctttaat cagttcgttg cctgcaaatg ccatgtcgtc ttcgtttgtt ccaacaatca
     8281 aaagattatg cgaatcatgt gcaacggttg atgccacagc tcctgtaata aaaccaaatc
     8341 ctgtaacaaa accaatgcct tttgtaccgg ttgccttgtg tctttcaaaa agtgcagctt
     8401 ttgcaatatc ttttgttgga tcaggtgcta tgttgccatt gacaacaggc aggatttcaa
     8461 atacctgttg tgtaagtacg cttgcttcct gtatttttat aacacgaacc tttgcctgcg
     8521 tcttattgct tttcaggata aaatcgtctt ttgttaatgt cttcccaaca ttcaccgtgt
     8581 tttttgacca atcaggatac gttacctttt ttgccgggat aagtattgtg ccatcctttg
     8641 caacaagctt tccaccaata atgacagctt tgacggttac cttataaagg tcagatagta
     8701 gtactatatc agcaaccttt gatggtgata cactgccaaa atccttactc atcataaagc
     8761 attcagcagt atttaaggtg accatctcaa tggcagtgac agggtcaaca ccttcttcaa
     8821 tggctcgctt tacgatatga tctaaatgtc ccaattcaat aagagtgtgc ggatgtgtgt
     8881 catcactgac aagaacagca aagcggctat ttactgtatt ctgggtgata gcttttataa
     8941 cttcttttaa atcatgccag gcagagcctt cacgtatctt tgcatacatt ccaaggcgca
     9001 tctttgcaag cgcatcttca gcacgcactg attcgtgaca tgagcgcaca ccgcttgcaa
     9061 catacgcatt gagcattgca ccagtatcgg gaagagcaaa atgtccggtg acaggtttgc
     9121 cagcctttaa tgttacttct atttctttgt gtacttccgg gacactgttg aaaactccgg
     9181 gaaaattcat aagctcaccc agcccggcaa taccctccca ctgcatggcc tgttcaatgt
     9241 cactggctcc aataactgct cctgcatctt ctaaccccgg tgcagaaggg acgcatgaag
     9301 gaaccgtagc atatactcta agaggcacat cttttgcttc atcaagcata agtcttacgc
     9361 ctttaagccc cagtacattg gcaatctcat gagggtccat gaagattgcc gtagtaccgt
     9421 ggggtaccac agcgttactg tactgactca cggtgagcat actgctttca acgtgaatat
     9481 gtccatccat gaaacctggc gctataaaaa gtcctgttgc atcaactatc tccgttgact
     9541 caccaataca gtgctttgca tcgccaacca gtgcaatgcg tcctttatat agtgctacat
     9601 caatatgttc ctgtaattct tttgtattta catttaccag tgttccgttt tttattacca
     9661 tatcagcctt gcggcgaccc atggcaacat cagccagttc catagttatg ttttctaatg
     9721 gtatgaagtc cattctgtgc cccttttaaa gataataact tttatgatta tacttgatac
     9781 aacaacgtta tccaatcttt tcaaaggctt ttgtaattgt ttcgtcagaa ggctttttag
     9841 taaacaagct aacaagtgga accacaacaa aaggtgccag catccctgcg cttgaagcta
     9901 taggtgaaag gtttgcacct aaatagaaaa acaggccaat tgacgttaca agccctgtaa
     9961 tcatacctgc atatactcct gcaagagttg tgcgcttcca gaaaagaccg tacaggtatg
    10021 gtgccataaa agcaccggca acagctcccc atgaaagtga catgagtgta atgattatgg
    10081 caaattcata tcgggcaata aaatatgaga taataataaa aagagcactt aaaaatcgca
    10141 tcatggcaac agagttttct ttagaaattt gtggattaac atgcccttta tacaggtcaa
    10201 tggctatagc cgacgacgat accagcacca gtgatgaaag tgttgacatg gatgctgata
    10261 atattaacag gagtataacc gccatgagtg gttcggggag gttttgtgtt aacaggtcgg
    10321 ggataagcct gtcaaatgct ggtttgccat caatagctgg aacagcatca taaaatatgt
    10381 gcgatagtga cccggtatag taggcgctga cgccaataat ggtagcaaaa atccccgtta
    10441 ctatgcttgc tttttttatt aaatcttcac ttttaatggc gtaaaatttc tgtaccattt
    10501 gtggcattcc ccagactcca aacgacgtca tgaaaatgag agcaccaagc aaaagatatc
    10561 caggatgctt atcggctggt atgtgcaatg gatatttttg tgcaaccagt gcaatacttt
    10621 gggctatccc accacttttt gctgaaagta ctaaaaccat ggtgatagct ccggcaatca
    10681 tgatgatacc ctgaataaaa tcagtcaatg taatagcaaa atatccaccc aaaaccaggt
    10741 aaataccggt aattcctatc atgatgatta atgccaggtc atacgaaata tggaaattaa
    10801 cttcaaacag atgccccaat cctttaaaaa ctgatgcaga atagggaagt aaaaatataa
    10861 aaattataat tgctgcaaac atcttgatgt atgtaccatt gaaacgttcc atcaaaaatt
    10921 caggcattgt catgacatct aaattttgtg tcatgatacg ggtacgcctg ccaagaacca
    10981 accatgcaat aaatgaacca aaaagagcgt tcccaacacc aatccatatt gcatcaagtc
    11041 caaaaaccca gcccagttta ccggcaaaac caataaacag cactgctgaa aagtatgacg
    11101 tgccatacgc cagagcagac acccatgggc caattgttct tccacctaaa aagaaatcac
    11161 caagtgtgga agtctttttc atgccccaga ctccaataaa aaccattagc actaaaaaga
    11221 ttgtaagtaa agaaattgac cacattgcca gttctccttt tttatatata gaatggtgtt
    11281 acatgcaata tgatatgata aaactgctat agtattatag cagaaacaat gtaagtattt
    11341 ttatatgttg caattagatg tcaatgataa aaaatctggc ggtagtaaaa caaaaggcgc
    11401 cttttttagg gcgccttttg tttctttcat tattcattca tggtgtactg gccttttaat
    11461 gcatacacgt ggtccttcca cactttgttg aagttttcat catcaagaac aggttttttg
    11521 atcatcttta atataagctc attctgttta tcggtgttct tctggcctgt gtaaagacgt
    11581 gttgcgtatg ctgaaaaatc acttacaatg aatttgaaga tttcatcaat aatctcatct
    11641 tcaacattgt aaatcttctt gttttcacag ataagctgtg tgtaaggaat aagcgtgaaa
    11701 agctcgccaa ctgtcagcat gtagtcaata ttcttggttt gcttttcatc aggtgttcct
    11761 ttaataagga aatttttaaa ggcttctatc tgttccataa atacttctaa atttttgctc
    11821 ctgatacctt catacgcttt gcggtaatca tggaactgga ttgaacccaa tcctttggtt
    11881 ggtccttgat tgaaaaggaa gctgtcattg gatggatcat cacgccgggg cacttcagga
    11941 tagtcttttg gtttgaacat gaagttttgt aaaaatttta caaccagcgc catgttgacg
    12001 tgagtggtac cctctaattt gggcaacatg ccaatatcgc ggattgccat ctcaaagtag
    12061 gtttcctgtt caaagccgcg tgctgcaata acctcatgta gcagcccaat aaccttttcg
    12121 ccttccatgg taactttcat tttctggata gggttgtata gtaaataccg gcggtcattt
    12181 tcatttgcac tacgcatgta atcacaggct cttaatgcaa aaagcttcat ggcagcaagt
    12241 cgtgcaaatg cttcggtaaa aatcttttta acatgcggaa aatcagttac atacatatta
    12301 tatagatttc tgttagcagc gtggtcaata gcttcataga aagcatgttc gcagatacca
    12361 attgaagccc agcccaattc gtacttacca acattaactg tattgagtga agaatcccat
    12421 gcgtgattgc cacgtgacag gatgtcagct tcggttatgg gatagtcatg gagagcaaac
    12481 tcagcaacat acgcctggcg cacaccagaa gtgcttatct ttttaacgca ttcatagttg
    12541 ggatgttttg tgtcaacaac aaagaataca tattcgccgg tatcagccat tttgccaaaa
    12601 actgaaatga gtgctgctgc attgccattg ccaatgtaat acttgtcacc acgagctaag
    12661 tatgtgccat cacctttggg atagagcatc atgtcggtgc tgtagatatc agcgccatgc
    12721 tctttttcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa aacagaatat
    12841 ataagtatga aggaagttat gagcagattg caaaagttat ttaccaggga acaaatagtg
    12901 atgagttttc tatgattcag ttttataaaa tgatagtaat gaattattta tattttgaaa
    12961 aatggagatg ctcatctaca aaattttgat gtattgtatg aacctgatat gacaaaatac
    13021 acttatcacc tgcatatgat gtagttgcca ctgttgttta tatttataaa gacaaacctg
    13081 cactaacatt gtgtggaaga aaactatggg caggcctaca tgaattaaaa aaagtttggt
    13141 caaaaatttt tctttttgtc tgaaaatgat agcaatatga tcattgaaga atgcagataa
    13201 gcagtaaaag atactattac ggaactaaag aaatatattc ttgacaatag tacttttgca
    13261 ggtattggac aaagaattat tgatgtgtgg gaatttttat taactcaaac taaaaccata
    13321 tgggagatcc cagatgacat tctacgaaat tggtgaagag ataaaaagaa tacgaaaaca
    13381 aaaaaaaatc acacagggaa tactgtgcaa aacaggtatc agcagaccaa cgttatcaaa
    13441 aattgaacaa ggtgaatttg ttaatgtgag tgtcagagca cttgataaaa ttcttgtggc
    13501 tctggggtat gaactatcgt tgcaaccaag aaatatcgtt ggcctgccac cattacagga
    13561 tgattaattg aaaattatac ttgcaatttg caccaaccac tgtagactat cgctatgatt
    13621 aaacgaaaga tagaaaaaac gcttaaagag cttgcaaaac aatatccggt tgtaactatt
    13681 acggggcccc gacaaagcgg aaaaacaaca ttatgcagat atgtatttaa acataaaaac
    13741 tatgtaaatt tagaagctcc tgacataaga atgtttgcac ttgaagatcc acgagggttt
    13801 ctaaaacagc atggaaataa tgttattatt gatgaaatac aaagagtgcc acagcttata
    13861 tcatatatac agacaatagt tgatgataat cctgaatccg gacagtttat actgactggg
    13921 agcagccatt ttgggattat gaatgggata agtcagtcat tagccggccg aacagcaata
    13981 ataacattat tgccgttcag ttatgaagaa atacagcata tcgtcccctg taacattgac
    14041 agattgctat atacaggatt ttatcctaga atacacgata agaaattgaa tccaacacag
    14101 gcactggccg attatgttgc aacatatgta gaaagggatt taagacaact ggctgctatc
    14161 aaggaccttt acacatttga aaaatttttg cgtttatgtg caggtagggt agggcagctt
    14221 ttaaatatgc agagtctggc aagtgatgtt ggcgtatcgc acacaactat acgtgcgtgg
    14281 atatcaatac ttgaagccag ttatattata tttttgcttc aaccatggta tggcaatgta
    14341 gcaaaacgtt tgataaaatc accaaagcta tatttttatg atgttggact ggcaacctat
    14401 ctccttggta ttgaaaatga aaaacagatg caacgcgatc cattacgggg caatcttttt
    14461 gaaaatatgg tcatagctga aattataaaa tatagattca accgtggtaa aaggagcaat
    14521 atattttttt accgggatag caaaggtaca gaggttgatg tactgtacca atatggaagg
    14581 aatatattcc ccattgaaat aaaagcaggc gctacagttg tggatgattt ttttaaaaat
    14641 tttaatgcag tatgtaagta tattgattgt gaaaaaggcg cggttatata tggtggcact
    14701 gaaattcaaa aaagaactgg cgttgatgtt attccgtttg ttaaaatccc tgatttatta
    14761 caacaaatag ataaaaatca ataaactatt gatgtctgga atcactgtaa gttattgaca
    14821 ataattgtgt atattgtagt gtacgatagt ggttgtaaat ggtcaaagaa accggatgga
    14881 tgatgtagct atttaaagag cagtgatatt aaccttgtcc atacattcca tgtaaggcgc
    14941 ggaattacga ttttcaggaa ttatactatt gagggaatat atgaaacgag cactcattac
    15001 aggcattact gggcaggatg gtgcatattt agcagagttg cttttgcaaa agggatatac
    15061 cgttattggc ggattcaggc ggttgagcac tccaaacttc tggcgattag aggagcttaa
    15121 tattcttgat aagattgtca tggttgaaat agatatgctt gatgctggca acctggtgcg
    15181 cgtcatagaa aaatataaac cggatgaagt gtataattta gctgcgcaga gttttgtggc
    15241 acactcattt gcaaatcctg ttcttacggg taatgtcacc ggccttggtg taaccaatat
    15301 tctggaagca atccgaatag tgaataaaga cataaagttt tatcaggcat caacatccga
    15361 gctttttggc aaggcacagg aaactccaca aaaagaaaca acaccatttt atccccgcag
    15421 tccctacgct gtggcaaagc tatacggtca ctggatgaca ataaactatc gcgaagccta
    15481 tggcattttt gcttccacgg gaattctttt taaccatgaa tccccattgc gtgggattga
    15541 gtttgtaaca cgaaagatca cggatgctat cgccaaaatt taccatgaga agcaggatta
    15601 ctttgaaata ggcaatttag atgcaaagcg tgactggggc tatgcaaaag aatttgttga
    15661 gggtatgtgg cgtattttgc aggctccgcg ccctgataat tatgtgcttg ctaccggtga
    15721 aacgcattct gtgcgcgaat gggttgaggt gtgttttaac tatattggga aaaatattgt
    15781 gtgggacggc acaggcgtgg atgaagttgg caaagacgca aaaagcggta aaacgctgat
    15841 aaaggttaat cctgaatttt tccgcccggc cgaggtagat tacctggtgg gagatgcatc
    15901 caaagcaaag aaggaactgg gctgggagcc aaaagtaaaa tttaaagagc ttgccgaaat
    15961 aatgctcaag cgtgatattg aacgaaatag atagacggct attttcaact caaattgaaa
    16021 atagtcttga ctttttccca gtttattgta aaatagtcgc aatgattgca agaaatatac
    16081 aattatgtta tttccgctga cagatttttg cagtatcttt gttaatacaa ttaaaaaaaa
    16141 tggaattgct aattccaaaa atgcagcaaa aatggaattg acaattccat aaatcaatat
    16201 gagtataaat atagtatagg gtttatgcag caaatcctga gcaattaaaa gaattacaag
    16261 tatgaataca tttgagcgta ttaaaaaaga atgtttctgg gattataaaa taacaccgga
    16321 agaaattaaa ataatagccg aaagcggtac aaaagaagaa aagaaaaaat tatttcagaa
    16381 aatcatgtac aactcccgtg ataaacttgg ccatttgatg atttttaaga aagatgagct
    16441 tagggaattt ttcaacgatt ttgttccagc gtataatacc atgtatttac gtaaacatat
    16501 gaaagtatta aaatatttgt tactgaaaga acatgaaccg gtacaagagc ttgcatggaa
    16561 agagaaatag attatacggc tttatatgca ttacaggatg atgttttgaa ggttattttt
    16621 aatctggaaa ataattttta cattacagga gggactgcac ttcaccgatt ttattacaat
    16681 gcacggtatt cagacgatat tgatctgttt gtaaataata gttttacttt tcatgaagaa
    16741 gtacaggaaa tttttgaagc tatcgcaaat aacgggtttg atattgaaag gtctgtgagc
    16801 accagagatt tttaccgcat tatggtagac aacatcttac gccttgattt tgtaaatgac
    16861 agagtataca gacatggtaa aagtactata atcaattcca tacgagttga taacaaaatt
    16921 aatatattaa caaataaaat aacagcaatt atcaataggg atgaagaaaa agatatattt
    16981 gatttatttt gttttgcata taatgaaaat tttaattggg aagaagtctt gcacattgca
    17041 aataaaaaac atccggttga aaaagacatt cttgtttatc gattgaaaac gtttccgctt
    17101 aattggttaa agagaataaa aacaataaaa tcagtttcta taactccgga aaaaatagca
    17161 atactgtgtg atgatattat taataataca cagaacagct tggtgggttc cagttttaac
    17221 caataattaa atccttaaaa aatttcattg cgagcacacc gtagcaattc acgtcattgc
    17281 gagcgcaagc gaagcaatct catgatatta tataatcttg caggctcatt gccctgtaaa
    17341 ttccagtgca tgctaccggc caaacgcatt ttgtgcgtga atgggttgag gtgtgttttg
    17401 aatacattga taaaaatatt gtatgtggcc cacggtaggg cagtaaaaca ccatatgcta
    17461 tacaggaaat gcattaaaaa atataatgaa aaagctgcac cataatgcaa tggttcaata
    17521 tgttattttc gccgacagat ttttgcagta tctttgttaa tacaattaaa aaaatggaat
    17581 tgctaattcc aaaaatgcag caaaaatgga attgacaatt ccataaatta gtattagtgt
    17641 gacgccatga tatttgtaca gcgttttata ctacctcctc caaccagttt ttttcttttt
    17701 ggcccccgcg gtacaggaaa gtcattatgg gtaaagaata catttgtaaa tgccctgtac
    17761 atagatcttc ttgatccgga atatttcagg gtttatacag catatcccga gcgattaaaa
    17821 gaattactga atgctcaacc gcaaaaaaaa gatattatta tagatgaaat acaaaaagtc
    17881 ccgtatcttt taccattggt tcattcgtta attgaggaaa aaaagggata ccgttttatc
    17941 ttaacaggtt caagttcacg gaagcttaag cgaagtggtg tggatttatt agctgggagg
    18001 gctcttcact atacaatgca tccatttttg ccatctgaat tgggtgagca ttttatattt
    18061 gaaaaagcac tacagtatgg tctggtgcct cttgtatatt tttcagatag tccagagcaa
    18121 acgttagctg catacgtatc tctctatcta aaagaagaaa tacaggccga agcgttaatc
    18181 aggaatatcc ctgcgtttac acggttttta gaagcattga gtttttcgca tgcgtcagtt
    18241 ttaaatgtaa gtcaggttgc acgtgaatgt atggttgaac gcaaaacagt tgaaggctat
    18301 cttaaagttc ttgaggattt actattatgt tttacaattg gtgttttttc aaaacgagca
    18361 aaacgaaaat taattagcca tagcaagttt tatttttttg atacaggagt attcagacat
    18421 ctgcgcccta aagggccttt agacaggttg tctgaaattg atggtcagtc attagaagga
    18481 cttgtggcac agacactgag aacctggatg cattacagaa acaaaaataa cacattgtat
    18541 tattggcgga ctatcaatgg gttagaagtt gattttattg tatatggtga agatggtctc
    18601 tatgcaatag aagtaaaaaa tgccagaagc atttaccccg tggatttaaa agggctggaa
    18661 gcatttaaaa aagattatcc tgaagcacaa tgtattcttg tttaccgggg caatgagcgg
    18721 ttacagaaag gtgatgtagt atgtattcct gttgaagaat ttttatataa gcttcatccg
    18781 gataggccta tcatgaatat actataaaaa attaaaaaac tctgtgcttc cctgtgctct
    18841 ctgtggtgga aaggaataac cacagagagc acggagtggc acggagatgg atgtggctgg
    18901 tagttcttaa aaatcctcac attttttatt gacaaaataa aaaagattgc ccatttatag
    18961 ttacatgcgt gtactataca atatagtgca tcagccggga aggccagctc attttcgccg
    19021 ttaagaaaaa ataatgttat ttttagggga atattgtgga aacggcattt tatatttata
    19081 tttttgtttt tcccttcatt atttcaactg ttttaattcc agttattata caatttagcc
    19141 ataaaaacgg tttatatgat aaagtagatg caagaaaggt tcataagggt aaaataagta
    19201 gactgggtgg acttgctatt tttgcaggtt ttgtgagtgg ttttatatgt ttacttattt
    19261 atgattttca agtccaattc aatattgtgg tattcattat agccctttta cttgcttttt
    19321 ttactggctt cattgatgac gttattacca ttagagccat gtataagctg cttttgcagc
    19381 ttgtttgtgg tcttttggtg gcactttcag ggttaaagat tacaaaagtt acattatttg
    19441 caggttttga tgtacagttt gggatattat catatttgtt taccatggta tgggttgcag
    19501 gatttatgaa tgcaatcaat atgctggatg gtatggacgg acttgcatcg ggtattgtgc
    19561 tggtggcatc aatgtttctt ggaataatgg gctggcttca gggaattcct attgtgacat
    19621 atctatgtgt tgttttgatt ggggcaatac taggattttt attgtataat tatcctcccg
    19681 caaagatttt tatgggtgat gggggagctt attttttagg gtttatatat gcagtgcttc
    19741 cgctcattgg tattaaaaaa gcttcaacta ttaccatatt ttcaatccca ataattctat
    19801 tgttgattcc tcttattgat gtcataaatg ttatgcgaaa aagaattaat gcgggatatc
    19861 atatattcat tgcagataga aaccacatcc atcatcggct ccttaaaatt ggcttcagca
    19921 atgaagggat tttatgggtt atgtacagct atactgtgat tcttggagga tttgcggtct
    19981 taatgctgta tctatcgccc ttgcatgcta taatccttac agtttttctt tttatgttaa
    20041 caattcttac tttctacata atatcagttg ctgaaaaacg aattgaagag ctggaaggaa
    20101 gaagtgttaa acaacaaaaa acacaaaaaa taaaaacact gcaaaaagta aaaaattcaa
    20161 ttatatacaa aaagttattt acataaataa atgttttaca aaattatagt atgtatgtaa
    20221 aattataata gatttggttg tagaaaatgt gttacgtcaa agattaaaaa caattaagcc
    20281 agttttttca tttcaatgac atggctaaaa attaaaaatt ttaatttata ggagaggtat
    20341 ttgttttatg gaagaaagaa atgtaatata tgacgatgaa attgatctct atgagttagt
    20401 tgtcaaacta tggaaatata aaacatttat aatattattc acaatttttt gtactgttgc
    20461 atcacttgtg gcagttaatt ttataacacc acgatataaa gtaacttgcc aggttgtacc
    20521 caataatgat gaatttgtaa aattgttttt gcgagaaata aataaataca acgatcccaa
    20581 actatggctt tctgatataa gatttaatta ctatcaggaa ataattgctc ctctttttca
    20641 aagtagaaac cttctaatga gagcaattaa agatgccctt ccaggtgatt ttgatcatgc
    20701 tttttggatg gaagttttag atgaacaatt attggatgta aaagttgaac gggacaaaca
    20761 attaaataat gtgataacta taggtattga atatacgaat gctgaattag tatataaaat
    20821 tgcaaaaaat ttattagtta aagttgaaga tgaattgaaa acaatgtatg ctattaacaa
    20881 ctcaggaaaa caacttctta tagtaactca aactcctaca ttacccaata aaccatgtag
    20941 cccaaagaaa accttgatta ttgctgtgac ttttgtaact tcactttttt ttgctgtgtt
    21001 tatggcgctt attgttgatt ggatacgcac agcaaggaag cagtatttaa gagagcaata
    21061 ataaataata caatatatct ttgatttgtt tttacaatca acaagtgtaa aaagtttttt
    21121 aaaaatcttg acaaaaatgg ataattattt tactcagttt taatgggtct attttatagg
    21181 tttttaattg ctatttttaa cgtgtttttt ttcataaagt tcaatgtaat ttatgctact
    21241 ttatttatat atttttttgt agcttaatga cgtgtaatat caattcttca aaatattttg
    21301 ctctgttaca aaatgccact gtaactgaga gggcgaagct ttgtggtcat cctgagtccc
    21361 gatgaaatcg ggacgaagga tctcttttta gaaaacggtg agattcttcg ggacttcgtc
    21421 tttcagaatg acaggaggtt cgggctgcgt tactcaggta tacaaaggag atagtcgtaa
    21481 cagtggttgt aaatgcccgt ttattaaaac acaaagaaca aaaaagaaaa aaatcatgtc
    21541 atatttcaat atcattgaca ggtgtctaaa tgatatatat ttttaatata tatcaagagg
    21601 tggctttatg ctaaaaatag caaaagtatt taaaaatggt cgtagtcagg caattagaat
    21661 accaaaagag tttaagatta cttcaaaaga agtatatatt gaacaagaag gtaataaaat
    21721 tataatatat cctaaatacg atggcatatg ggatgcattt tttcaaaaac tgaaagaggt
    21781 tgacacgaat ggttttttgg aagaaaggaa tcaattgcct cttcaaaaaa gggacatttt
    21841 attatgatct acatgctgga taccaatgtg tgtagttata ttattcgtga gaaaccctat
    21901 catctaaagg ataaaattgt aaaggtttat aaagaacata cagtttcgtt atccagtatt
    21961 gttgttgcgg aactgctata tggcgcgaaa aagaaaaata gcaaagcatt aacgcaaata
    22021 atcgaattgt ttattgaaaa ttttgtgata tttgattttg acaggaaagc agctgttgaa
    22081 tacgcatcaa taagggatag tttggagaaa tcaggaaaca taatagggtc taatgatctt
    22141 tttatagcag cgcatgcaaa atcattgaat gcggtgcttg tgaccaataa cgccaaagag
    22201 tttaaaagaa taaaggggct taaagtagaa aactggatgt agtgaagcag aaaaactcaa
    22261 ggagttttat atgacaacaa aaaaacctat tttta
//
LOCUS       DAORYL010000051        22114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_1989_length_22114_cov_13.670883, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000051 DAORYL010000000
VERSION     DAORYL010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 22114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22114
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1989_length_22114_cov_13.670883"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="PLV81_09935"
     CDS             complement(<1..1058)
                     /locus_tag="PLV81_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM10455.1"
                     /translation="MPLPKSYRRHHRLFHRAPLPGLDASIRKEIMEYTMNLLQHLEPN
                     ERLLTLCMECLSITSRREIVESLFSEKEKKNRNEFGPNYYEDALGSLHNPSDLASTIM
                     RIVLSAAQKKKWDTVRYKIFMLIKKEYETVAQYKHELDERLSKLAAFFSLTDVDCTIM
                     KLWAIAISIPNSPLKELLSEVNYSELQKRIAIATGLPLAAVRMSLSKKGLLYTSRIIE
                     SINPDGYEHIVLSDEIIEFLNDVASEDLLEKYVKLDTEPTISLQEFSVSEDDKYIVTN
                     LLKSKQPCNILLYGFPGTGKTQFARSIAAHSGHKVFMLVYTSGRESTRHESISSRIMA
                     VTIAMKIAEKNDAILIID"
     gene            complement(1227..2180)
                     /locus_tag="PLV81_09940"
     CDS             complement(1227..2180)
                     /locus_tag="PLV81_09940"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOM10456.1"
                     /translation="MCSMVRKNNNNFPNPEGKINVLKRYLHILALLQSPPGTNETWNA
                     ARLAQLLSLEEDEKDISDSHVRKYIKDHIENELGISVDRTQGGLITALAEDLDPDMQL
                     EIARVYSSFVVKDSTRDMILQKFIATNPDRALWTLARVYFAALEKRMIQCSYKTNEGQ
                     LLTDWKLCPCYFIFRNNNLYLIVYDVDMKKYLPLVAERIQNIVVCDKKHDISWEVPDV
                     DELFATSLSAFIDNTNTVEVTIRYSQVVKTIIENIIASLQPAIQKEPNNWHRATFTIS
                     DYLYLCKQLVLYGREVEIVSPHKVRQAMIHMLKESLSVYEQ"
     gene            2591..4333
                     /locus_tag="PLV81_09945"
     CDS             2591..4333
                     /locus_tag="PLV81_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylpolyamine amidohydrolase"
                     /protein_id="HOM10457.1"
                     /translation="MIRIRRLFNPYIPHNIDAFNAVKNIIKEQFPLLAIKKIDEIEDQ
                     LINPLKYKYHTSLFIAEDIHDSIKGVALVLHFTDLGIFYLDYLAVKPGKSSGGIGSAL
                     YQRIRKEASLLNVFGIFLECLPDDPLLCNNPEDVEQNKKRLAFYERFGARPIINTLYE
                     SKVKPDDDCPPYLVFDGLGNYDSIPNKLARNIIQAILERKYNDYCDEKYITRVIESIK
                     DDPVQLRPFRYIHNKTLIHNIVTPHTYTIRLYINDRHHIHHVKDRGYVESPVRVKSIL
                     SELLKSDIFENGKISHYPDKFIYEVHNKNYVDYFKNVCKNLPEGKSVYPYVFPIRNAA
                     RAPRELSVRAGYYCFDTFTPLNKNAYLAARWGVNCVLSAVDDIVSGIPVAYVLTRPPG
                     HHAEHSVFGGFCYFNNVAIAAQRFSKVGKVAILDIDYHHGNGQQQIFYKRNDVLTISI
                     HGHPSFAYPYFSGFAEELGEENGKGFNYNFPLPETISYDKYKHTLLRAVKIIKDFNPD
                     FLIVALGLDVGKGDPTGTWGLVKEDFYTNGGIIATCGIPTLIVQEGGYRNLTIGKNAF
                     AFFMGFYNSFTLKK"
     gene            4435..5361
                     /locus_tag="PLV81_09950"
     CDS             4435..5361
                     /locus_tag="PLV81_09950"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOM10458.1"
                     /translation="MVQRYKFINENFLQNDCGNIVVYSNPNESEISTIASDFGIDIHS
                     VHSALDIDELSRFEIEKDYIVLIWKIPTNIKINELSSLNVITIGFFIKDSTMIIISPE
                     TLTYIHEKVHNQIESLFDVLFHFQHHTIKHFTEHLKIIKMISKEIQNKINKSIETKHL
                     VQMFNLSEKLIYYLHAIDTNITSLEKLRSYLITNNQRVNFDFLNDIIIDHHQAKKQAE
                     IYTEVFAGLMDARGSIVNNNMNILIKNLTKINVIFLPLNLIAGIFGMSEYSMMTQGIP
                     WYISYGFFAILIMFVSLLLSLLLNKYDERWRR"
     gene            5383..6360
                     /locus_tag="PLV81_09955"
     CDS             5383..6360
                     /locus_tag="PLV81_09955"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HOM10459.1"
                     /translation="MVINGTHYTLHLAPMAELTTPALRKVVKNFTDNVVLYSEMLASV
                     AIHAKAPHNEPLIRRYDFDNPFFYQLVGNNPDIMANAATILQDYGCDGININMACSAP
                     HILKTGSGASLLCNFNLARSIIAACRKNVHGLLTVKMRAGFHDVNLQFLRQFVSMLQD
                     EGVDAVIIHPRAAKWGFTRKARWDIIEAIVTEVNIPVIGNGDISEPASALHRLQQTHC
                     DGIMIGRAAVQKPWIFAQCEAALRQQSLSLSLNIEEIWIDVLQNIQVMLPQRLHKSRA
                     HRFCAYYHKNVKFGHTLFSSIRQCNEINDMIVLIRNYFKRNEEERINNV"
     gene            complement(6364..7335)
                     /locus_tag="PLV81_09960"
     CDS             complement(6364..7335)
                     /locus_tag="PLV81_09960"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HOM10460.1"
                     /translation="MAENIHDKGYKYLFSNPLMVKELLESFVYMDWVKEVDFTQAETI
                     DKTYVNDYYKEYEADIIYKLRFKNIDMYIYLLIEFQSTVDKFIAFRMLQYIVELYREL
                     IYSHNVKKLPVVFPIMIYNGDKVWKAPLTLEELIDIPEILHECLQYVPRFRYYPLIEN
                     ELNPVILQKMMNVISTVFLLETGDDAIFYKTIDRIKDLVQIYTEKGEFLLIRTLLYWL
                     LHYLQSHGIVDGDEKISENLIKTPQEATSMLAQTLKKIKSDLIKEGFNKGIEKGIQKG
                     IKQGKLIGKHEERIAIARNLLKSGIDDKTIIKITKLTKDQLKELKNR"
     gene            7556..8452
                     /locus_tag="PLV81_09965"
     CDS             7556..8452
                     /locus_tag="PLV81_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOM10461.1"
                     /translation="MIYPSYIALYERGELHKRIEALNTIVTHCTLCPHACKVDRRSTT
                     GKCRTGLQALVASYHAHFGEESCLVGTYGSGTIFFANCNLTCIFCQNWDISQMPNGHE
                     VTPEELADMMIRLQHKGCHNINFVSPTHVVHAIIEALPFAIERGLTIPLVYNSGGYDS
                     VETLQLLDGIFDIYMPDFKYMDATAAMELSGAREYPQKAIAALKEMHRQVGDLVIEKG
                     IAKRGLLVRHLVMPENIVRSDLVFKELSAISPHTYVNIMAQYRPEYRAHTHKSLGRRI
                     TIQEYDQAYLWAKQSGLYRFDR"
     gene            8500..9552
                     /locus_tag="PLV81_09970"
     CDS             8500..9552
                     /locus_tag="PLV81_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOM10462.1"
                     /translation="MLKSSAQLINLFDLVNDGILIIDGNNYNVLFNNDRAEKFVTKDG
                     TTSILEDEKIKNILRSLQPDKTHAEHISLKINNKIHHYKVNILAIKDEESYLIAFVLT
                     DTTRLFKLHRKQQQLIAQIRKNYFDRFESLKQLADSIAHEVRNPLVSIGGYANLLMRK
                     CEEGIDHSEAKKFLSYIVENAERLNYLTQQIEEMGDLKRVQLSAYDIVKFLQEQKTTL
                     SAAAKQFNKDLHITIETENHYHMYIDYDRLRFAFTRIIEFIAQHAKKDTIRMHCHDSV
                     YEFIITISFSTDILAEEDLPYIFDPFYTTRSNIETFNLCIAQRIIMLHGGIITPELKK
                     NSIIFKIALPQDKRLT"
     gene            complement(9544..10830)
                     /gene="hemL"
                     /locus_tag="PLV81_09975"
     CDS             complement(9544..10830)
                     /gene="hemL"
                     /locus_tag="PLV81_09975"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HOM10463.1"
                     /translation="MHTLRSKELYEEACKYMPGGVNSPVRAFRSVGGHPLYFKLGKGS
                     HIIDEDGNDFIDYCMSWGPLILGHADNDVVKAIQKVAENGTSFGAPHEGEISIARMVV
                     ESFPGIEKVRFVNSGTEATMSAIRLARGYTGKSKIIKFDGCYHGHADHLLVAAGSGLA
                     TFGMPDSAGVTRDNARDTMVVKYNDIDALHQVLSHNDDVACCIMEPVCCNYGLILPKK
                     DYLKQVRELCTKHKVLLIFDEVITGFRLARGGAQERFGVQADITTLGKIIGGGLPVGA
                     YGASSEIMAKVAPDGPVYQAGTLSGNPIAVAAGIATLQKLVPSAYQKLEELGTYFEEQ
                     MKPILSRFKNKVLFMRFASIFALYFTNEDIITSVDQVKECNMQQFARFHRLMLERGIY
                     LSPSGYEVGFLSLSHSKDDIDKTVKAIGESLEIVLR"
     gene            complement(10855..11829)
                     /gene="hemB"
                     /locus_tag="PLV81_09980"
     CDS             complement(10855..11829)
                     /gene="hemB"
                     /locus_tag="PLV81_09980"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOM10464.1"
                     /translation="MHPYYRPRRLRETEVIRNLCAETVLDYNKLIMPYFVIEGAHINQ
                     PIESMPGVSRLSIDNLLKTLEADVASGVQSIILFGIPYEKDEKASGAYDDNGIVQKAC
                     REIKKHFKHITIITDVCLCEYTSHGHCGMVVNGRVDNDSTLELLAKTAVSHAQAGADV
                     VAPSDMMDGRIEAIRTALDEAGLFHIPILSYAAKYASAFYGPFRDAAGSAPQFGDRRS
                     YQMNFRNAREALKEVYLDIEEGADIVMVKPALAYLDILYRVKQAVDVPVCAYNVSGEY
                     SMVKAAATLGFGDEARLVREIMTAIFRAGADMVISYHTVDILKNRWFD"
     gene            complement(11863..13344)
                     /gene="cobA"
                     /locus_tag="PLV81_09985"
     CDS             complement(11863..13344)
                     /gene="cobA"
                     /locus_tag="PLV81_09985"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013910233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOM10465.1"
                     /translation="MKQHGFVYLVGAGPGDPGLISVKGKECIEKADCIIYDYLANPVL
                     LQHTRAELIYVGKQGSQHTLSQEEINRLIVQKVREGKVVVRLKGGDPFIFGRGGEEAE
                     ELIAAGIAYAIVPGISSFYSALAYAGIPITHRDYAASFEVITGHRRADGTEDITLPDY
                     SQQKTYTFLMGMKNLDTICKRLIQEKNFPAQTPIAVVTWGTLPKQKVATGTLETIASE
                     VEKAGLTPPAIIAIGRVVSLRNTLRWYDTLPLFGKKVVVTRTREQASVLSRRLYELGA
                     DVVEFPTIAITKLPELSPLHNALNRLHDYDWIVFTSQNAVSIFFEELFALGLDARSLR
                     CKVAAIGPATRDALRAYAVVADLMPQEYVAESLVDAFKGVDIEGKKILVPCSVKARAA
                     LTQGLQQAGAQVERVHIYDATRPEVTPAMLQQVQNADIITFTSSSTVRNFVELAGTTT
                     ATVACIGPITADECIKQGLSPHIIAKEYTIEGLVQAILEYFTG"
     gene            complement(13341..14237)
                     /gene="hemC"
                     /locus_tag="PLV81_09990"
     CDS             complement(13341..14237)
                     /gene="hemC"
                     /locus_tag="PLV81_09990"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOM10466.1"
                     /translation="MIKIGTRGSALALWQAHFVAGQLTQEHTIVTIKTKGDRIQNVSF
                     DKIEGKGFFTKELEEALLSGTIDMAVHSMKDLPTDDTPGLTIAAVTKREDPSDVIIIR
                     PEAYRANHWFPLKENTKVGTSSLRRVAQLKRILSSAEAMPLRGNVPTRIRRLREAKFD
                     AIILAKAGLKRLGVDLSGFYSMVLPFSYFLPSPGQGALAIQVRSYDKTAIEAVTHLHD
                     EATAKSVQAERQFLKAFGAGCHVPLGAFAHCVDETIILTGVILSVDGTRYFRHTVEDT
                     DPQRAGDRLARFMIEQGAEKLL"
     gene            14463..15386
                     /locus_tag="PLV81_09995"
     CDS             14463..15386
                     /locus_tag="PLV81_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HOM10467.1"
                     /translation="MKILVYGAGAIGSIFAGKLIKAGFNVTVLARNERYNELKSNGLI
                     LKNVITGTIETYYPELIQNLYPDDIYDYIIVAVQYTQIDSILPVLAKNKSKNIVFVIN
                     NPSGYDKYINAVGYERILIGFPSAGGERLNGIVNYFIGKGISKLFQSTTFGELSGKKT
                     QRLLTLTNIFKKAKFSPEICSNMDAWQKTHIAVVMPISKALYKFNSDNYKLAQSYKTI
                     KMMVLATRECFKVLKMMKIPITPFKLNFYYLPTYIIVPIFMIIMNTKIAEFAMAKHTI
                     VAKDEMIALENQFKNFIDKLNIDTPSLQWLE"
     gene            15457..15651
                     /locus_tag="PLV81_10000"
     CDS             15457..15651
                     /locus_tag="PLV81_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10468.1"
                     /translation="MNLENLGYNETLEQLRIEHNLQHFEIGRIIAEHKERYVVNTPHG
                     EYDAEITGKLRHKPQATDNS"
     assembly_gap    15674..15773
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15774..16134
                     /locus_tag="PLV81_10005"
     CDS             <15774..16134
                     /locus_tag="PLV81_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOM10469.1"
                     /translation="VTSHRQLIILENGGILIDNPGMREVGIADASEGLETTFDDIYRL
                     AAHCKFNDCTHTSEVGCAVIHAVEHGQIDRKSYENYLKMTREKAFFESTVAERRQKDR
                     EFGRMMKNYKKARKNKF"
     gene            complement(16208..16798)
                     /locus_tag="PLV81_10010"
     CDS             complement(16208..16798)
                     /locus_tag="PLV81_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HOM10470.1"
                     /translation="MPLPKPQKDSKFTYADYLTWTGDERWELIDGVAYDMTPAPDVNH
                     QVIVGNTYQIISEYLEGKTCRVFLAPIDVRLPESDAADDAVYTVVQPDVVVVCDPEKI
                     EKRGIIGAPDLVVEVLSESTAYKDETEKLNLYERHGVKEYWIVNPGAKYINVFQLKSS
                     GRYSKPLHYRADETFESSVLPDLVIDCARIFKGADV"
     gene            complement(17085..18167)
                     /locus_tag="PLV81_10015"
     CDS             complement(17085..18167)
                     /locus_tag="PLV81_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10471.1"
                     /translation="MKRFTLAAVMCAIFVALTACGGGGSDDYTPSVYVAGAVSTGTGS
                     NACYWKDGLRVDLPGANGIAYGIAVANGHVYVVGQYSNSDTWCYWIDGVRYDLTGNGY
                     FSKIKVVGSAVYISGTKDNNACVWTNGIYYPVNYNNSYTYGMEIIEDTIYLLGGIGEG
                     GGHAAIWTKPVNGGAWTKYDLPDTGYEYSDAYALAAYDDMIYIVGYLYKDSQSYPALW
                     KGNSLGSLTVAELPTPNKDASDIYGYTYAIKIYNGQPYIAGHYNIINNSVGYHRAALW
                     IGNNDPTLYAPEDQDDSMAFDVCIYNGNVYVAGKIRKPKSNDPNTQYLQPCYWKDGVR
                     VDLSVLDEVGNSITDVSVSANAIVVQ"
     assembly_gap    18238..18337
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18738..20327
                     /locus_tag="PLV81_10020"
     CDS             18738..20327
                     /locus_tag="PLV81_10020"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOM10472.1"
                     /translation="MKTTLKKLFTTYTSFQNTKYGNIIIILVFSVIYSLVFNAIDFIR
                     YPVKYGNTVINTVLLFILSIPLVFYYFLVASIHKKLFTIVTLLTTFLSSIALYFIIVY
                     KVRIYLLDTFILLVQTNPHEAGGVISFDLIIMVLSITLATAVFLYYYNIQHNDALFRR
                     IKIIFILLLSPVLFLLYFSRPVMPYAFMYAINMYNKYQTIIHHPRTDISKLPSSFNEE
                     KNHNLTVIFIIGEAARADHFSINGYPRKTSPNIEKLQVVSLPNIDSIHGVTNKVVPLM
                     MTRATKENEEITYQETSFVSIFNKHGFTTAWISNQDVIDNSYSNIAPFANEATIKKQI
                     IWNKNSKKDFSVTNANILDENMLPELDELLKINNKKLVILHCMGSHWEFHKHYPDTFR
                     KFVPICTTSNVSRCNRDELINNYDNSILYADYFISEVIKRVMNKNAIVLYCSDHGEFL
                     GEDGYYGHIPGIQRKEITNPAMFVWMSEEYKKRNPEKYTNLLKNKNKHLPNYILFHSI
                     LDAASIHSTAANYKLSIFSRM"
     gene            complement(20352..21983)
                     /locus_tag="PLV81_10025"
     CDS             complement(20352..21983)
                     /locus_tag="PLV81_10025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOM10473.1"
                     /translation="MDTIAQKNERMMLLLLLVFIVIYKFFTFRMFDIGGDAINYWFAA
                     KHIYYNVPYIELDHQTARFGLILPVWFSQLIFGAHPAVSLIIPNLFFIIHMILLYKIG
                     VKIKSPATGFITVLLYMLCPYIIRFGTQVVPEAVGGPLVTASFYLLLQYKDDNKKELL
                     YLTLSAFLLFWAYCAHELDIFLMPGYILVILLFKRNMKHAIFYSTVLFILFLFETSVF
                     YYYTGDIMARYHAITGHHFTLEKFVPISFWQLFERYTKTQLWWRIPFYIYLVLLYPVW
                     KKSNVEIKSIIIPGFIFFLCMLFAVKSINPIVPALAYKEVHLLFALPFMLFVIAIVID
                     DSVHVIYKKSNLLQRLVKPSIEPIKIWTAVIVFLTFISLIVFSTNVLPEKASPYYNNP
                     LRIKEHKFLRFFTYYSIFNDAYEKDVPIVSDYVYYVKDKEIYDNVQKLRSQGKNLTEA
                     LQLAGVDLQYYTSIRPNMPVKSLNAVRKIFLNVNPLNIPGIRTLQVGDRILYFIIKRD
                     LSVLEQKKLFIEGSPVVYATGRELKALKETFRVNL"
BASE COUNT         6988 a         4303 c         3739 g         6884 t
ORIGIN      
        1 tcatcaataa tcaggattgc atcatttttt tcagctatct tcatagctat ggttacagcc
       61 ataatgcgcg aacttatact ttcatgcctg gtactttcgc gtccactggt atagacaagc
      121 ataaagactt tatggccact atgcgcagct atgctccgtg caaactgggt tttacctgtt
      181 cccgggaatc catatagaag aatattgcag ggttgtttgc tttttaataa atttgttact
      241 atatacttat catcttctga tacgctgaat tcttgtaacg atatcgttgg ttcagtatct
      301 aatttaacat atttctcaag caaatcttca cttgcaacat cattcaaaaa ttcaattatt
      361 tcatccgaca gcacgatatg ttcataacca tcaggattta tagactcaat gatgcgagag
      421 gtatataaaa gccctttttt tgatagggac attctaacag cagcaagcgg caatcctgta
      481 gcaatggcaa tgcgtttttg caattcactg taattaactt cagacaacag ttcttttaac
      541 gggctgtttg gtattgaaat agctatagcc catagcttca taatggtgca atcaacgtca
      601 gtgagtgaaa aaaatgccgc taatttagat aaacgctcat ctaattcatg tttatactgt
      661 gcaacagttt catattcttt ttttatcaac ataaaaatct tgtaacgaac tgtatcccat
      721 tttttctttt gtgcagcact cagtacaata cgcataattg ttgaagctaa gtctgaagga
      781 ttatgcaagc tccctaatgc atcttcataa taatttggac caaattcatt acggtttttc
      841 ttttcttttt cagaaaacag tgattctact atctctctgc ggcttgtaat acttaaacat
      901 tccatacaca gtgttagcaa gcgttcgttt ggttctaaat gctgcagtaa attcattgtg
      961 tattccatta tctctttacg tattgaagca tccagtcctg gcagtggtgc tctgtgaaaa
     1021 agtctatggt gacgtcgata tgattttggt aaaggcatag ctacctcctg taatggtaat
     1081 atagggtact ttgttaacac cgtggttgtt gaagatagat cctttatgag taactatcgc
     1141 tactacattt agactacgta tggcaggtta tttattggta aaatactata agcatagtaa
     1201 aaaaggtagc aactgacttg cgatgtttat tgttcataca cgcttaagct ttccttaagc
     1261 atgtgtatca tggcctgtcg taccttgtgt gggcttacta tctctacttc tcttccatac
     1321 agcacaagct gtttgcacag gtaaagataa tcgcttatgg taaatgttgc tctgtgccag
     1381 ttattgggct ccttttgtat tgcaggctgt aacgaggcaa taatattttc aatgattgtt
     1441 tttactacct gggaatagcg aatggtaact tccactgtat tggtgttatc aataaatgcg
     1501 ctcagtgacg ttgcaaataa ctcatcaaca tcagggactt cccagctaat atcatgtttt
     1561 ttatcacata ccacaatatt ctgtatgcgc tcagcaacaa gcggaaggta ttttttcata
     1621 tctacatcat acactataag atacaggttg ttgttacgaa atataaaata acagggacac
     1681 agtttccagt cggttagtaa ctgcccttca ttggttttgt agctacattg tatcatgcgt
     1741 ttttcaagcg ctgcaaaata gacgcgtgcc aatgtccata gtgccctatc aggatttgtt
     1801 gcaataaatt tttgcaatat catatcacgc gttgagtcct ttaccacaaa gctgctataa
     1861 actcgcgcaa tttcaagctg catgtcagga tcaaggtctt cggcaagtgc tgttatgagt
     1921 ccgccctgtg tacggtcaac acttattcct aactcatttt ctatatggtc tttgatgtat
     1981 tttcggacat ggctgtcgct aatgtctttt tcatcttctt caagcgatag taactgcgca
     2041 agtcttgctg cattccaggt ttcgttggtt ccaggtggtg attgcagaag cgccagtata
     2101 tgaaggtagc gcttcaatac gttgatcttg ccttcagggt tagggaagtt gttattgttt
     2161 ttacgaacca ttgaacacat atgtttcaca gtacgaaatt gtttgtaaac tatttttttg
     2221 aattcttttt actgcagaaa agtttgcata tccctggtca aatcatatac tctctataaa
     2281 ctgacagaaa aatttattag caaaattgtt ttaagaagta atcatctaaa tccttcaaaa
     2341 ttaatttttt aaatttccac caggttaata atatagaaca caatgtttaa aaatattcac
     2401 ttgccatacc ttttattgtg cataatgctt taatcataaa atactctgat ggcaatttgt
     2461 gaaatatttt tgtaaatctt gttatatatt atatactata ctatataata gcactatctg
     2521 tgaaagatac acaatcacta ataacatgta tgatacaatg taaataaaaa aactaatcaa
     2581 aggaaaaact atgatacgta tacgccgatt gttcaatccg tatatacctc ataatattga
     2641 tgcttttaat gcagtaaaaa atatcataaa agagcaattc ccgcttctgg caattaaaaa
     2701 aattgacgag attgaagatc aactcatcaa cccccttaaa tataaatatc atacatcatt
     2761 atttattgct gaagacatac atgattctat caaaggcgtt gctctggttt tgcattttac
     2821 tgatttgggc attttctacc ttgattatct tgctgttaaa ccaggaaaat cgtctggggg
     2881 tattggttca gctctttatc agcgaatccg taaagaagcc agcttgctga atgtttttgg
     2941 aatttttctg gaatgtcttc ctgatgatcc tttactgtgt aataatccag aagatgttga
     3001 acaaaataaa aaaagattgg cgttttacga acgttttgga gcacgtccca taataaatac
     3061 tctctatgaa tccaaagtta aacctgatga tgattgtcct ccatatctgg tttttgacgg
     3121 attggggaat tatgattcta taccaaacaa attggcacga aatattatac aggcaatact
     3181 tgagagaaaa tataacgact attgtgatga aaaatacatt acacgtgtta ttgaaagtat
     3241 aaaggatgat cctgtacaac tacggccatt tagatacatt cacaataaaa ccttaataca
     3301 caatattgta actcctcata catacaccat tagactctat ataaacgata ggcatcacat
     3361 acaccacgta aaagacaggg gatatgttga atcacctgta agagtcaaaa gtatattgtc
     3421 cgaattatta aaatcagata tatttgaaaa tggaaaaata tcacattatc ctgataaatt
     3481 tatatatgaa gtacacaata aaaattatgt ggactatttt aaaaatgtat gtaaaaatct
     3541 acctgaaggg aaatcggtat acccgtatgt tttcccaatt agaaatgctg ccagggcacc
     3601 cagggaactt tcagtacgtg caggatacta ctgctttgac acatttacac cactcaataa
     3661 aaatgcttat ttagcagcac gatggggagt taattgcgtc ttatctgctg ttgatgatat
     3721 tgtctcaggc atcccggtag cttatgtcct gacccgccct cccggtcatc atgcagagca
     3781 ttctgtattt ggaggttttt gttattttaa caatgttgct attgcagctc agcgattcag
     3841 taaagtaggc aaggttgcta ttctggatat agattatcat catggcaatg ggcaacagca
     3901 aattttttat aaaagaaatg atgtcctcac aatatccata catggtcatc cttcgtttgc
     3961 ctatccttat ttctccggtt ttgcggaaga gcttggtgaa gaaaatggca aggggtttaa
     4021 ttacaatttc cccctccccg aaactatctc atatgacaaa tacaaacaca ccctgttgcg
     4081 tgcagtgaag attataaaag attttaaccc tgattttcta attgtagccc tgggattgga
     4141 tgtgggaaaa ggcgatccta caggaacatg gggtctggta aaagaagact tctacaccaa
     4201 tggaggaata atagcaacat gtggaatacc aacactcatt gtacaggaag gtggctatag
     4261 aaatcttact atagggaaaa atgcatttgc gtttttcatg ggattttata atagttttac
     4321 attgaaaaaa taaattaaat tttatactac atatagtact gaatccttca tgtatttatt
     4381 tttttcctgc aatgtagttc tttactaatt attcttttta cgtatggagg acatatggtt
     4441 caaagatata aatttataaa tgaaaatttc ttacaaaatg attgcggcaa cattgtagta
     4501 tactcaaatc ctaatgaaag tgaaatatcc actatagcca gtgattttgg catagatatt
     4561 cattcggttc attctgctct cgatattgat gagctatcac gatttgaaat tgaaaaagat
     4621 tatatagttc ttatatggaa aataccaaca aatatcaaga ttaatgaact ttcatccctc
     4681 aatgttataa ctataggctt cttcataaaa gacagtacaa tgattatcat cagtcctgaa
     4741 actttaacat atattcatga aaaagtacac aatcagatcg aatcactttt tgatgtactg
     4801 ttccattttc aacaccatac aataaaacat tttactgaac atctaaaaat cattaaaatg
     4861 atttcaaagg aaatccagaa taagattaac aaatctatag aaacaaaaca ccttgttcag
     4921 atgtttaacc tcagcgaaaa gttaatatac tacctgcatg caatagatac caacattact
     4981 tcccttgaaa aattacggtc atatcttata actaataatc agcgtgttaa ttttgatttt
     5041 ttaaatgata ttatcattga ccaccaccag gcaaaaaagc aggcagaaat ttatacagaa
     5101 gtttttgctg gtcttatgga tgcccgtgga agcattgtga acaacaatat gaatattctt
     5161 attaaaaatc taacaaagat aaatgtaata tttctacctt taaatttaat agctggtatt
     5221 tttggcatgt cagaatactc aatgatgacc caggggatac catggtacat ctcatatggg
     5281 tttttcgcaa ttttaattat gttcgttagt ttgttgttaa gcttgctact aaacaaatac
     5341 gacgaacgct ggcgaagata aaaacaacat gcttaataca ctatggttat taatggcaca
     5401 cattacacac tgcatttagc gcccatggca gagcttacca caccagcgct gcgtaaggtt
     5461 gtcaaaaatt ttactgataa cgttgttctg tattctgaga tgcttgcaag tgttgctata
     5521 catgcaaaag caccccataa cgagccttta atacggcgct atgattttga caatccgttt
     5581 ttttatcagc tggttgggaa taatcctgac attatggcca acgctgcaac aattttacag
     5641 gactacggat gcgatggcat taatataaac atggcatgct ctgcaccgca tattcttaaa
     5701 actggcagtg gtgcaagcct gctttgcaat tttaacctgg cacgatctat tattgcagct
     5761 tgcagaaaaa atgttcacgg ccttcttact gttaagatgc gtgctggctt tcatgatgtt
     5821 aaccttcaat tcttgcgtca atttgttagt atgctgcagg atgaaggtgt ggatgcagtc
     5881 attattcatc cacgtgctgc aaaatgggga tttactcgca aagctcgctg ggatatcatt
     5941 gaggcgatag taactgaggt aaacatccct gttattggca atggcgatat cagtgaacca
     6001 gcatcagcac tacatcgttt acagcaaacc cattgtgatg gcatcatgat tggcagagca
     6061 gcggtacaga aaccatggat atttgcacag tgtgaagctg cattgcggca acaatcgctg
     6121 tcgctatcgc ttaatattga agaaatatgg atagacgtat tacaaaatat acaggtaatg
     6181 cttccgcaga ggcttcacaa aagcagggca caccgttttt gtgcatatta tcataaaaat
     6241 gtaaaatttg gccacacatt gttttcatca atacgacaat gtaacgaaat aaacgatatg
     6301 attgtactta taagaaatta ttttaaacga aatgaggaag aaagaataaa caatgtataa
     6361 tggttaccta ttttttagct ctttcaactg gtcttttgtt aatttagtta ttttgataat
     6421 tgttttatca tcgattccgc ttttcaataa attgcgtgca atagcaattc tttcctcatg
     6481 cttccctatt aacttgccct gtttaattcc tttttgaatt cctttttcaa tccctttatt
     6541 aaatccttct tttattaaat ctgattttat cttttttaat gtttgtgcta acattgaggt
     6601 tgcctcctga ggagttttta tcaaattttc agatattttc tcatcaccat ctacaatacc
     6661 gtgtgattgc aaataatgta ataaccagta taataatgta cgtataagca aaaattcgcc
     6721 tttctctgta taaatttgca ctaaatcctt aatcctgtca atagttttat aaaatattgc
     6781 atcatcacct gtttccaaca aaaacacagt agatataaca ttcatcatct tttgaagaat
     6841 tacagggtta agttcatttt caattaatgg ataatacctg aacctgggga catactgtaa
     6901 gcattcatgt aatatttctg gaatatcaat caattcttca agtgttagtg gtgctttcca
     6961 tactttatca ccattatata tcataatagg gaatacaaca ggcaattttt taacattgtg
     7021 agaatatatc aattccctgt ataattctac aatatattgt aacatcctga atgctataaa
     7081 tttgtctaca gtagattgga attcaatcaa tagatagata tacatatcaa tatttttaaa
     7141 acgcaattta tagataatat ctgcttcata ttctttatag taatcattta catacgtttt
     7201 atcgatagtt tcagcctgag taaaatcaac ttctttaacc caatccatgt agacaaatga
     7261 ttccaacagt tctttcacca ttaatggatt ggaaaataaa tacttatacc ctttatcatg
     7321 gatattttca gccatagata cctcatggta gaaatctatt gtatttatat ataaatacgc
     7381 ttcaggataa aaaatttgaa aaattatgat aaaaattttt gagttttttt aatgaatctt
     7441 taaaattgtt atacgcatat aatgaaaaga aagcatgatg tactaattta attattttgt
     7501 ttattaaatt gcctggtatc aataaacata atacttattg aaaatagtag catccatgat
     7561 ttatccatca tacatagctt tatatgaacg tggagagctt cataaacgca ttgaagcgct
     7621 gaacactatc gttacccact gtacgctctg ccctcacgca tgcaaagttg acaggcgttc
     7681 tacaaccggt aaatgcagaa ctggtttgca agcactcgta gcttcatatc atgcacactt
     7741 tggtgaagaa tcatgccttg ttggtacata cggctcaggg acaatctttt ttgctaattg
     7801 taaccttacc tgcatattct gccagaactg ggatatatca caaatgccaa acggccacga
     7861 agtaacccct gaagaactgg ctgatatgat gatacggctg cagcacaaag gctgccataa
     7921 cataaatttt gtaagcccaa cacatgtggt ccatgcaatt atagaggcac tcccttttgc
     7981 cattgaacgt ggccttacta ttcctctggt atacaattca ggtggttatg attcggtaga
     8041 aacactacaa ttgttagatg gtatatttga tatttacatg cccgatttca agtatatgga
     8101 cgctacagct gcaatggaat taagtggtgc cagagagtat ccgcaaaaag ctattgccgc
     8161 ccttaaagag atgcatcgtc aggttggaga ccttgtcatt gaaaagggca ttgcaaaaag
     8221 aggtttgctg gtacgtcatt tagtcatgcc tgagaacatt gtccgcagtg accttgtttt
     8281 taaagaacta tctgctattt cacctcacac ttatgtaaat attatggctc agtaccgtcc
     8341 tgaatacagg gcacataccc ataaaagcct gggaaggcgt attaccatac aggaatatga
     8401 tcaggcttat ttgtgggcaa aacaatcagg attatacagg tttgatagat aactttattt
     8461 tttattgacc tcaatttttt ttacatatat attataccca tgctgaaatc ttctgcacaa
     8521 ctcatcaatc tttttgacct ggtgaatgac gggatactca tcattgatgg taataattac
     8581 aatgtacttt ttaacaatga cagggcagaa aagtttgtta caaaagatgg tacaaccagt
     8641 atcctggaag acgaaaaaat taaaaatatt ttacgttccc tacaacctga taaaactcat
     8701 gcagaacata tcagcttaaa aataaataat aaaattcatc actataaggt taacatatta
     8761 gcaattaaag atgaagagtc ttacctgatt gcatttgtat taactgatac tacccgttta
     8821 tttaaactcc atcgtaagca gcagcaactc attgcccaga tacggaaaaa ttattttgac
     8881 cgttttgaat cactgaaaca gttagcagac tcaatagctc atgaggtacg gaatcccctt
     8941 gtttccatag gtggatatgc caatctcctg atgcgaaaat gtgaagaagg gatagatcac
     9001 tccgaggcaa aaaaattttt atcctatatt gttgaaaatg ctgaacgact gaactacctg
     9061 actcaacaaa ttgaagagat gggcgaccta aaaagagttc aactatctgc atatgatatt
     9121 gtaaaatttt tacaagaaca aaaaacaacg ttaagtgcag ctgcaaaaca atttaataaa
     9181 gatttacaca taactattga aactgaaaat cattatcaca tgtatataga ttatgaccgc
     9241 ttacgttttg cttttacccg catcattgaa tttattgctc agcatgcaaa aaaggacaca
     9301 atacgtatgc actgtcatga tagcgtttat gaatttatta tcaccatctc gttttctacg
     9361 gacatacttg ccgaagaaga tttaccctat atttttgatc cattctacac aactcgatca
     9421 aatattgaaa catttaacct ctgtattgcc cagcgtatca tcatgctgca cggtggaatc
     9481 attactcctg aacttaagaa aaactccatt atattcaaaa ttgccctacc acaggacaag
     9541 cggttaacgt aatactattt ctaacgattc accaattgct tttactgttt tgtcaatatc
     9601 atcttttgag tgcgataagg ataaaaagcc aacctcataa cccgatggcg aaagatatat
     9661 accgcgctca agcattaacc tatgaaagcg tgcaaactgc tgcatattgc actctttaac
     9721 ctgatcaaca cttgtaatga tatcttcatt ggtaaagtac aatgcaaata ttgaagcaaa
     9781 gcgcataaac agcactttat ttttaaaacg cgataaaatc ggtttcatct gctcttcaaa
     9841 atatgtgccc agttcctcaa gcttttgata tgctgatggc accaattttt gcaacgttgc
     9901 aatgcctgct gcaacagcaa taggattacc tgaaagtgtt cctgcctgat acacaggccc
     9961 atcgggggca acctttgcca taatttcaga cgatgccccg taagcaccaa caggtagtcc
    10021 cccgccaata attttaccaa gtgttgttat gtcagcctgt acaccaaaac gctcctgtgc
    10081 accaccacgg gcaagcctga aaccagtaat aacctcatca aatatcaaaa gaactttatg
    10141 ttttgtacat agctcccgca cctgcttcag gtaatctttt tttggaagaa taagcccgta
    10201 gttacaacat accggctcca tgatacagca cgcaacatca tcgttgtgcg atagtacctg
    10261 atgtaaggca tctatgtcgt tatatttcac taccatggtg tctctggcat tgtccctggt
    10321 tacaccagct gaatcgggca taccaaaggt tgcaagccct gaacctgcag caaccagcag
    10381 atgatcagca tggccatggt agcaaccatc aaattttatt atcttgcttt tgccagtata
    10441 cccgcgtgca agtctgatag cactcatggt tgcctcagta ccagagttaa caaagcgaac
    10501 tttttcaata ccagggaatg attcaaccac cattcgcgcg atgcttatct ccccttcatg
    10561 tggtgcacca aagcttgtgc cattttcagc tactttctga atagctttaa caacatcatt
    10621 atcagcatgc cctaaaataa gcggtcccca tgacatgcag taatcaataa aatcattacc
    10681 atcttcatca atgatatgac tacccttgcc taatttaaaa tacagtggat gcccaccaac
    10741 cgaacgaaat gcacgcactg gtgagttaac accgccaggc atatatttac atgcctcttc
    10801 ataaagttct tttgaccgta atgtatgcaa tgccataatc cccccttaca taaactaatc
    10861 aaaccacctg tttttcagga tatcaacggt atgatatgaa atcaccatat cggctccagc
    10921 cctaaaaatt gctgtcataa tttcacgtac caggcgtgcc tcatcaccaa accctaatgt
    10981 ggctgcagct tttaccattg aatactcacc actcacattg taggcgcata cgggaacatc
    11041 taccgcctgt ttaacacggt atagtatatc taagtatgca agtgcaggtt ttaccatgac
    11101 aatatcagca ccttcttcaa tatcaagata cacctctttc aacgcttcac gtgcatttct
    11161 gaaattcatc tgatagctac gcctatcacc aaattgcggt gcgctccctg ctgcatccct
    11221 gaatggtcca tagaaagccg aagcatattt tgcagcatat gataaaatgg gaatatgaaa
    11281 taatcctgct tcatcaagtg ctgttcgtat tgcttcaatg cggccatcca tcatatccga
    11341 tggtgctacc acatcagcac ctgcctgtgc atgcgaaaca gctgttttgg ccagcagttc
    11401 caatgtactg tcattatcta ccctaccatt aactaccatg ccacaatggc catggcttgt
    11461 gtattcacac aggcacacat cagtaataat agttatgtgt ttaaaatgtt tttttatttc
    11521 cctgcaggct ttctgtacta taccattatc atcatacgca ccactggctt tttcatcttt
    11581 ttcatacgga atgccaaaga gtataataga ctgtacacct gacgcaacat cagcttccaa
    11641 tgttttcaaa agattatcaa ttgataggcg gcttactcct ggcattgact caatgggctg
    11701 attaatatgt gccccttcaa ttacaaaata gggcatgatg agtttattgt aatcaagaac
    11761 tgtttcagca caaagatttc gtattacttc tgtttcacgt aaacgccgtg gcctgtaata
    11821 gggatgcata gtagtactcc tataataatg aaatgtaaaa aactatcctg taaagtactc
    11881 cagtatagcc tgcaccaatc cttctatagt atattctttt gcaataatat gtggcgatag
    11941 tccctgtttt atgcactcat ctgctgtgat tggacctatg caggcaactg ttgctgtagt
    12001 ggttcctgca agctccacaa aattgcgcac cgttgatgaa ctggtaaatg taatgatgtc
    12061 agcattctgc acttgctgta gcattgcagg tgttacttca ggcctggtag catcatagat
    12121 atgcactctt tccacctgcg ctcctgcctg ttgcaggcct tgagtcaatg cagcccgtgc
    12181 ttttaccgaa cagggaacaa gaattttttt gccttcaata tccacaccct taaacgcatc
    12241 caccaggctt tcggccacat actcctgtgg catcagatca gcaactactg catatgcccg
    12301 taatgcatca cgtgttgcag gaccaattgc tgccacctta caccgcaatg agcgcgcatc
    12361 caaacctaac gcaaacaatt cttcaaagaa aatgctcacc gcattctgtg atgtgaatac
    12421 aatccagtca taatcatgca acctgttcaa agcattatgg agtggcgata gttccggcaa
    12481 ttttgttatt gctatggtag gaaactccac cacatcagca cctaactcat acaaccgtct
    12541 ggaaagaacg cttgcctgct cacgcgtgcg cgtcaccacc acttttttgc caaatagcgg
    12601 caaggtgtca taccagcgca acgtatttcg caagcttact accctgccta ttgcaataat
    12661 ggctggtggc gttaatccag ccttttctac ttctgaggca atagtttcca gtgtgccggt
    12721 tgccaccttt tgttttggca aggttcccca ggtaaccact gcgattggtg tttgcgccgg
    12781 gaaatttttt tcctgtatca gtcgcttaca gatggtatct aagtttttca ttcccataag
    12841 aaatgtgtat gttttctgct ggctgtaatc aggtaatgtg atatcttcag tgccatcagc
    12901 tcgcctgtgt ccggtgatta cttcaaagct tgctgcataa tcacggtgag ttatggggat
    12961 gcccgcataa gccagtgccg agtaaaagga cgatataccg ggaactatcg cataggctat
    13021 acctgctgct attaattctt ctgcttcttc accgcccctg ccaaagataa atggatcacc
    13081 accctttaat cgcaccacca ctttaccttc gcgcaccttt tgcacaatga gtctgttaat
    13141 ctcttcctgc gacagcgtgt gctggcttcc ctgcttgccc acatagataa gctcggcgcg
    13201 ggtgtgttgc aataatactg gatttgctaa atagtcgtag ataatgcaat cagctttttc
    13261 aatgcactct ttgcccttaa ccgaaatgag acccggatca ccgggacctg ctcctactaa
    13321 atacacaaat ccatgttgtt tcatagtagt ttttctgctc cctgctcaat cataaagcgt
    13381 gctaacctgt cgcctgctcg ttgtgggtca gtatcttcaa cagtatggcg aaaataccga
    13441 gttccgtcaa ctgaaagaat gacaccggta agtataattg tttcatcaac acaatgtgca
    13501 aatgcgccta atggcacgtg gcagcctgca ccaaacgctt tcaggaactg tcgctcagcc
    13561 tgaactgatt ttgctgttgc ttcatcatga agatgcgtta ctgcttcaat tgctgttttg
    13621 tcataactgc gtacctgtat agcaagcgca ccctgtcctg gtgaaggcaa aaagtagctg
    13681 aatggcagca ccatactgta aaaccccgac agatcaacac ccaaccgttt taatcccgct
    13741 tttgccagta tgatggcatc aaacttagct tcgcgcaggc gacggatgcg tgtgggcaca
    13801 ttgcctctga gtggcatggc ttccgctgac gacaaaattc gtttcagctg tgccaccctt
    13861 cgcagtgagc ttgttccaac ttttgtattt tcttttagcg gaaaccagtg gtttgctcta
    13921 tatgcttcag gccgtataat gattacatcg gaagggtcct cacgttttgt gaccgctgca
    13981 atggtaagtc caggcgtgtc atcggttggc aggtccttca tggaatgcac agccatgtcg
    14041 atagttcctg ataacagggc ctcttccagt tccttggtaa aaaaaccttt gccttcaatt
    14101 ttatcaaagg acacattttg tatgcgatca ccctttgttt ttatggtgac tattgtatgt
    14161 tcctgtgtga gctggcctgc cacaaaatga gcttgccaca acgccagtgc actaccccgt
    14221 gttcctattt ttatcatagt tacttatcta tcacatttta ctgaaatttc acaacaacaa
    14281 actgtatgtg ctggtaatgt agtatggtta attcatttga gattgtgtca aataaaaatt
    14341 gggaaagtat tgaacaagaa ttcgtcaatt ttgtttttat acaaaattgt atatcaatat
    14401 caatcatgtg ctatattgaa ttaagacctt gcaatatggt aaataacatc aaggagacaa
    14461 tgatgaaaat attagtgtat ggagccggag ctataggtag tatttttgct ggtaaactta
    14521 ttaaagcagg atttaatgtt acagtactgg cacgaaatga aaggtataac gaacttaaat
    14581 ccaatggttt gatactaaaa aatgttatta cgggcacaat tgaaacgtat tatcctgaat
    14641 tgatacaaaa cctttaccct gatgatatct atgattatat cattgtagca gttcaatata
    14701 ctcaaatcga ttcaatttta cctgttcttg caaaaaataa atcaaaaaac attgtgtttg
    14761 taatcaataa tccttctgga tatgacaaat atattaacgc cgtaggctac gaacgcattt
    14821 tgattggctt cccttcggct ggaggtgaaa ggctgaatgg tattgtaaat tattttattg
    14881 gtaaaggaat ttctaaatta tttcaatcaa caacatttgg tgaactcagt ggtaaaaaga
    14941 cacaaagatt attaacatta accaatattt ttaaaaaagc aaaattttca ccagaaatat
    15001 gcagcaatat ggatgcatgg caaaaaacgc atattgcagt tgtaatgccc atttcaaaag
    15061 cactatataa atttaattct gataactaca aactggcaca atcatataaa acaattaaaa
    15121 tgatggtatt agcaaccagg gagtgtttta aagttttgaa aatgatgaag atacccataa
    15181 caccctttaa gctaaatttt tattatttac ctacatatat tatagttcca atttttatga
    15241 taattatgaa tacaaaaatt gcggagtttg caatggcaaa gcatactatt gtagctaaag
    15301 atgagatgat agctttagaa aaccaattta aaaatttcat agataaatta aatattgaca
    15361 ccccatcact gcaatggtta gagtaatatt gtgtaccagt gaattcttgt ttcgatatat
    15421 ttcatggtac ttagctcaat ttatggaaat acataaatga atcttgaaaa tttaggctac
    15481 aatgaaacat tagaacaact gcggattgag cacaacctgc aacattttga gataggaaga
    15541 atcatcgcag aacataaaga acgctatgtg gtgaacacac cacatggtga atatgacgct
    15601 gaaataaccg gaaaacttcg tcacaagcca caggcaactg ataattcttg aaaatggtgg
    15661 catattaatt gacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgtcaca
    15781 agccacaggc aactgataat tcttgaaaat ggtggcatat taattgacaa tcccggtatg
    15841 cgggaagtgg ggatagctga tgcatcagaa ggccttgaaa caacgtttga tgatatttac
    15901 cgccttgcag cacactgtaa attcaacgat tgtacccata ccagcgaagt tgggtgtgct
    15961 gtcatccatg cagttgaaca tggacagatt gaccgaaaat cctatgaaaa ttatttaaag
    16021 atgacaaggg aaaaagcctt ttttgaatca acagttgcag agcgcagaca aaaggataga
    16081 gaatttggca gaatgatgaa gaattataaa aaggcaagaa aaaataaatt ttaatgcatt
    16141 ttatgcatga tatattcctt ttaactaact cggggaaaac tgtaataccg ttgtatggaa
    16201 gatgcattca tacatcagcg cctttaaaaa ttcgcgcgca gtcaatcaca agatcgggaa
    16261 gcacactgct ttcaaaggtt tcatccgcac gataatgcaa tggtttgctg taccgaccgc
    16321 tggatttcag ttgaaaaaca ttaatatact tagctccagg atttacaatc cagtactctt
    16381 ttaccccgtg gcgttcatac agattgagct tctcggtttc gtctttatag gcagttgact
    16441 cagaaagaac ttccaccacc agatccggtg cgccaataat ccctcttttt tcaatttttt
    16501 ctggatcgca taccaccacc acgtcgggtt gaaccaccgt gtacactgca tcatccgctg
    16561 catccgactc aggcaaacgc acatcaattg gtgcaagaaa aacccgacat gtttttcctt
    16621 caagatattc tgaaataatc tggtaggtat tcccgacaat tacctgatga ttcacatctg
    16681 gtgccggcgt catatcgtag gccactccat caataagctc ccagcgctca tcacctgtcc
    16741 aggtgagata atcggcatag gtaaatttac tgtctttttg gggtttgggc agaggcatgg
    16801 atatttcctc atgattttta attaatgaaa atatactgat tcaggtgctt ttgtcaataa
    16861 taatgcaata atgtgttaca gtcctaacaa ccatttccat aattgaaaca ctccgcaggc
    16921 gctcaagcgc ttgagcgcct gcggagaata ttagacaata aaactgctat ggcaaatttc
    16981 ctctgtgtac tctgcattct cttcgtgata tatttttttt actcgcagag cacaaagagg
    17041 agtgctgagt tgaattaata ttaaattgca attgaataat catatcattg cacaacgatt
    17101 gcattggcac ttacagaaac atctgttatg ctattgccaa cttcatccag aactgataaa
    17161 tcaacccgta ctccatcttt ccagtaacac ggttgaagat attgtgtatt tggatcatta
    17221 cttttaggct ttctgatttt tcctgccaca tagacattcc cattatatat acacacatca
    17281 aaagccatag aatcatcctg atcttctggg gcgtataaag ttggatcatt gttaccaatc
    17341 cataatgcag cacggtgata tcctacgcta ttattaataa tattataatg tcctgcaatg
    17401 tatggctgac cattatatat cttgattgcg taagtgtatc catagatatc acttgcatcc
    17461 ttattaggtg tgggaagttc agcaactgtt aaggagccta aggaattacc tttccatagt
    17521 gcaggataac tttgactatc tttatataaa taaccaacta tataaatcat atcatcatat
    17581 gcagccaatg cataggcatc ggaatattca taaccagtat ctggtaaatc atacttagtc
    17641 catgctccac catttactgg ttttgtccag attgcagcgt gcccaccacc ttcacctatt
    17701 cctcctaata gataaattgt atcttcgata atttccatac catatgtgta tgaattatta
    17761 taatttaccg gataataaat cccattagtc catacacagg cgttattatc ttttgttcct
    17821 gaaatatata ctgcgctgcc cactactttt attttagaaa agtatccatt accggttaaa
    17881 tcatatctta ctccatcaat ccagtaacac caagtgtcag aatttgaata ctgacccacc
    17941 acataaacat gaccatttgc cacagctatt ccataagcta tcccgttagc acctggcaag
    18001 tcaacccgca aaccgtcttt ccagtagcat gcattagaac ctgttcctgt ggaaacagca
    18061 cctgcaacat atacactggg agtgtagtca tcacttcccc ctcctccgca tgctgtaagg
    18121 gcaacaaaga tcgcacacat cactgctgct aaagtaaatc ttttcatata cattccttcg
    18181 taataaaaat gtaaattcac tatcatactg cgactgcttc tcttaaaacg tcattgcnnn
    18241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntca ggacaatagc atacctttac
    18361 cgcatcacta taattataat gattacggtt attattaaat attgtttgaa ggttgaaaac
    18421 gtcagagagg atattttgac acccccttaa agcaactaaa cgcctaccat tcagcctgtt
    18481 tgtaatatgc ggagttttta tgtatgcaat ggttatattt ttacaatcag accaaatcat
    18541 tatacaaaat atttttaaat tcaccagaag tgcaacaatt tttagagcta tcgcaaaaaa
    18601 aatttttatt tggtttttac tgttgaggat aaaataatga agagctataa ttgtaaacaa
    18661 catatcaata aatacaccat tttttgtaac attgcatttt aatagaatat tatatagctg
    18721 atgacgaaag agtaagtatg aaaacaacgt taaaaaaatt attcactaca tatacttcat
    18781 ttcagaatac taaatacggc aatattatta ttatattagt gttttcagtt atttattcgt
    18841 tggttttcaa tgccattgat ttcatacggt accctgttaa atatggcaat actgttataa
    18901 acacagtatt attattcata ctttcaattc ctctggtttt ttactatttc cttgtagcat
    18961 ccatacataa aaaattattt actattgtta cactactaac aacatttttg agctccatag
    19021 cactgtattt tatcatagta tataaagtaa gaatctacct tttagacacc tttatcttac
    19081 ttgttcaaac aaatccccat gaagcaggtg gcgttattag ctttgattta ataataatgg
    19141 ttttgtctat tacgctggca actgcagtat ttttatatta ttacaatata caacataatg
    19201 atgcactatt cagacgtatt aaaatcatct ttatactgct actaagccct gtgctttttt
    19261 tgttatattt ttcaagaccg gtaatgcctt atgctttcat gtatgccatt aatatgtata
    19321 ataaatacca gacaattata catcacccac gtactgacat ttcaaaatta ccatcatcat
    19381 tcaatgaaga aaaaaatcat aacctcactg ttatatttat cataggtgag gctgctcggg
    19441 ctgatcattt ttcaataaat ggatatccac gaaaaacatc tcccaatatt gaaaaattac
    19501 aggttgtaag tttaccaaac atcgattcta tccatggtgt tactaataaa gtagtcccac
    19561 tcatgatgac acgagccaca aaagaaaatg aagagataac atatcaagaa acatcttttg
    19621 tttccatatt taataaacac ggatttacca ctgcatggat atccaatcag gatgttattg
    19681 acaacagcta ttcaaatatt gctccatttg ccaatgaagc aacaattaaa aagcaaatta
    19741 tatggaataa aaacagtaaa aaagatttta gtgtaaccaa tgcaaatatt ttagatgaaa
    19801 atatgctgcc cgaacttgat gaacttctaa aaattaataa taaaaaattg gtcattttgc
    19861 attgcatggg cagccactgg gaatttcata agcattatcc ggatacattc aggaagtttg
    19921 ttccaatttg tacaaccagc aatgtatccc gttgcaatcg tgatgagctg attaacaatt
    19981 atgacaattc aatactctat gcagattatt ttatatcgga agtaataaaa cgagtaatga
    20041 ataaaaatgc aattgtacta tattgttcag atcacggtga attccttggt gaggatggat
    20101 actatggtca tatacccgga atacaaagaa aagaaataac aaatcctgcc atgttcgtat
    20161 ggatgtcaga agaatataaa aagagaaatc ccgaaaaata tactaatctt ttgaaaaata
    20221 aaaacaaaca tcttcctaac tatatacttt tccacagcat cctggatgct gcttcaatac
    20281 atagtacagc agcaaattat aagctgagta tattttccag gatgtaacaa tctactgtta
    20341 caaatttatt tttacaggtt taccctaaat gtttctttca gtgccttgag ttccctacct
    20401 gtagcatata ctacgggaga accttcaata aacaattttt tctgttccag cacagataaa
    20461 tcacgcttaa taataaaata caaaattcta tcgcccacct gaagagtcct gatcccggga
    20521 atgtttaaag gatttacatt taaaaatatc tttctaaccg catttaaact tttcacaggc
    20581 atattggggc gtatagatgt ataatattgt aaatcaacac cagccagttg taatgcttcg
    20641 gtaagatttt taccctggga tctcagtttc tggacattat cgtatatttc tttatccttt
    20701 acataataca cataatcaga tactatcggc acgtcttttt cataagcatc gttaaaaatg
    20761 gaataatatg taaaaaagcg taaaaactta tgttctttaa ttcttaatgg attgttatag
    20821 taaggagatg ctttttcagg taatacattg gttgaaaaaa caatgagtga aataaatgtt
    20881 aaaaatacaa taacagcagt ccagatttta atgggctcaa tagatggctt tacaagtctt
    20941 tgaagtagat tacttttttt gtatataacg tgtacgctat catcaataac aattgcaatg
    21001 acaaaaagca taaatggcag ggcaaaaagc aggtgaactt ctttatatgc cagagcagga
    21061 acaatggggt ttatactctt aacagcaaag agcatacata aaaagaaaat aaagccagga
    21121 ataataatag attttatttc aacatttgat tttttccaga ccgggtacaa taaaacaaga
    21181 tatatataaa atggtattcg ccaccacagc tgtgttttag tatatcgttc aaaaagctgc
    21241 cagaaagata ttggcacaaa tttttcaagt gtaaaatgat ggccagttat ggcatgataa
    21301 cgagccataa tatcgccggt ataataataa aatactgatg tttcaaaaag gaacaggata
    21361 aataaaacag tgctatagaa tattgcatgt ttcatatttc gtttaaacag caatataaca
    21421 agtatgtatc ccggcataag aaaaatatcc agctcatgtg cacaatatgc ccagaatagc
    21481 aggaaggctg ataacgtcag atacaggagc tcttttttat tgtcatcttt gtattgtaat
    21541 aacaaataaa aagatgctgt aactaatggt ccaccaacag cttcaggaac tacctgtgta
    21601 ccaaaccgaa taatatacgg gcataacata tataagagca cagtaataaa acctgttgcc
    21661 gggcttttta tttttacgcc tattttgtac aggagaatca tgtgaatgat aaaaaataaa
    21721 tttggtatta tgagtgaaac agccggatga gcaccaaaaa ttaattgact gaaccataca
    21781 ggcaaaatta aaccaaagcg tgcagtctgg tggtcaagtt caatgtatgg aacattataa
    21841 taaatatgtt ttgcagcaaa ccagtaatta atggcatctc caccaatatc aaacattctg
    21901 aatgtgaaaa atttataaat aactataaac accagcagta ataacaacat catcctttcg
    21961 tttttttgtg cgatagtatc catgattaac tcccgtttga agaattataa taatatagca
    22021 attaacacat tgtttcacag gacaattcag atagctctgt aaatgttgca tttattataa
    22081 aataattgta atgaatcatt actgtataca ttta
//
LOCUS       DAORYL010000052        21605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_2049_length_21605_cov_13.988724, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000052 DAORYL010000000
VERSION     DAORYL010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 21605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21605
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2049_length_21605_cov_13.988724"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(381..1073)
                     /locus_tag="PLV81_10030"
     CDS             complement(381..1073)
                     /locus_tag="PLV81_10030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOM10474.1"
                     /translation="MKSWFYDESKHSGVNYASIELIQQYDENHSKFRNFAKEADEIIS
                     DLKLTTQSIVADIGCGTCAITQYIAKKVKKIYAIDISSPMLDYARQKYHTIHNIEYIH
                     SGFLGLHTHTFIVDAVYTKMALHHLPDFWKQMALKNISSITKPGGRLLYEDVIFSGDI
                     ETIEQNIDQWINQFKQQTGDEFAREVEMHVKEEFSTFDWAIEHMLHNAGYKIDARLNK
                     NEMIIKYICTKQ"
     gene            complement(1109..2158)
                     /locus_tag="PLV81_10035"
     CDS             complement(1109..2158)
                     /locus_tag="PLV81_10035"
                     /inference="COORDINATES: protein motif:HMM:NF025608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HOM10475.1"
                     /translation="MKRHQCSDPLFFLDYWFMQYYNSNTMLHTLKTIWHPEWYHNPAK
                     GPYFEGWYYKQVDKDGKHSFIVIPGIFIEKDKTKSYAFIHIFKKGQSPHFVTYPFHEF
                     TATYTPFSISIAGNHFTLNTITLAITSSDISVSGQCSFENRYPWPVKLFSPGSMGWYA
                     FVPTMECNHTVLSMDHSISGTIRYNEELIDFTGGRGYMEKDWGKSFPSAYIWFQTNHF
                     ATVGTSIFGSIATIPWKRSYFTGFLIALLHNGRLYTFTTYNNAQCTLSVTDLLVRISC
                     SKGNRNATITIKRTPGIALPAPYNGSMQSKIYESLDAHVHCVFNENNHTVLDDTGSHA
                     GFEIVGDIDNLIAHL"
     gene            2236..4092
                     /locus_tag="PLV81_10040"
     CDS             2236..4092
                     /locus_tag="PLV81_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10476.1"
                     /translation="MQTDIKALKRKAKALLLSKDIPAGKMDIVRAILANDRILEEEKY
                     NAIIDLVKNYPDKPVKPVAPGKVEVKGEEFKTPEEYNSKNTAYLYQKYRHTRLFKKRL
                     LVHANNKFGIGFLRRLVPSQRLLRLSQKLFELHYAMRQRLYRVLCDIVDDQSISDPTI
                     FNYCVVLYNALASSCLIDKQYIELKWTRPEGFKQLLSPYMNSMLKLSLCDAQTKEAMI
                     QLIDQKLRQSQDLQKNETMNKKNLTIEQYIYDFITTLRCLLYPSTTNECSLTNLLKKE
                     GSLPSFEAFCIACVEALVIGKPVVLSDIMTMFDITAPKVSDTEWNFDHDELVKAGKDP
                     ESLKKKAKEELHRKLEPYDELYRFLTYAPEGKEVAIQGFDDQRKLVDKRNEVFDDAYS
                     KDILIALEGMLLGFINEYGALLDGTTIYFENNKNHYEGCIFTENYFSHELARFSEILS
                     EINTFRSNNPTFHVTRDELIEGINKKKKSLYPIERFMTVIGDMCYDIGIELFTLYNRH
                     RRWVLSGSPGKDDAVMRTAFSAHTSGLSSLDDREAMPVPFSDCIITGFSKLRPLSKLL
                     LHKPVIHPTLRDGVFPQMVAYLLQMAHVCLNDRLYGILDERTKILSQIRSLS"
     gene            4089..5282
                     /locus_tag="PLV81_10045"
     CDS             4089..5282
                     /locus_tag="PLV81_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10477.1"
                     /translation="MRLLVILAMLSFALRAEAAPRVLVFKSYHDNLTSLLDEYRIPYI
                     VKRASVVAGDSSLSGFDAVFIPCGVEQPIEASIQVMAHGQRIQGVALKDDYEGIDFKR
                     LNALLQDFVKSGGRLYAAGYSYRFVEALGCSLSFFNNFPHYGTSGRIHSFLNDDFASF
                     VMRDDMQLTVNYNGWVALQRANASILAKGYFPTVGGEREGPVAFFKKYGNGVVYYSAY
                     HTGEDSLYMRYFIFRTAYGMLVDELLQKAWYWGQSIALSVVDTFKGDEYVRSYTCTLP
                     QGAITIYLPTTYPVQVDIFANKKLLGSWDSGFITEISLYNPATQDATVYVYQSNRAAY
                     KPFALVIAQGRRILPYMPVVRLSMGAIAVIMLLGFTWKRINPRKFAGKRRYWFRDGHF
                     IHRQK"
     gene            5640..6404
                     /locus_tag="PLV81_10050"
     CDS             5640..6404
                     /locus_tag="PLV81_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOM10478.1"
                     /translation="MGKIISISNQKGGVGKTTTAVNLAAFLAEKGKQVLVVDIDPQAN
                     AGFGLGVNVEEMETTVYEVLLGTIPVQEAIFRTTIENLYIVPSNMHLAGAQVELMDME
                     DKEYLLKRALEKVRDTFDFILIDCPPSLGILTLNSLVASDSVIIPLQCEYYALEGLSQ
                     LLKIISMVQDTLNPKLEIEGVLLTMYDPRTNLSQQVVSDVKQFFKNKVFSAIIPRNVR
                     LSEAPSFGKPINLYDRTSSGSMSYEQLAHELIQNVR"
     gene            6433..7302
                     /locus_tag="PLV81_10055"
     CDS             6433..7302
                     /locus_tag="PLV81_10055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOM10479.1"
                     /translation="MAKKVLGKGLGAIITTSPTPVSDFEKVLLQDKERIVDIPINAIM
                     PNPDQPRVHFDAAEIAGLAQSIQHVGLLQPIIVRKQGEEYFIVAGERRWRACKLLGKP
                     TIKAIVMNTTEEQNLTLALIENIQRTNLDPIEEAKAYKMLATRFKLKQSEIADRVGKD
                     RATVANIMRLLNLPEHMQEALQDGRINVGHAKLLLGVSGDEQEALFDRIIHQGLSVRD
                     VEKAIAEVPKTSGKKKKKAKDAHIRKLEEELISYLGTRVEIRHSAGKGRIEISFYSLD
                     DFERIVSLIKGDR"
     gene            complement(7303..7797)
                     /locus_tag="PLV81_10060"
     CDS             complement(7303..7797)
                     /locus_tag="PLV81_10060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HOM10480.1"
                     /translation="MKIGLISDTHNDIELVQKAIAIFKERNISVIIHAGDLTSSKIIS
                     LFKGFDGRFVLGNCDIDVEEINTTASECGFGCVEHSCQFTLDGKHFMVFHGNNVPMFR
                     EAVNSGAYDYIIKGHTHIFENYVSGKTRIINPGSLYRGEEHTVAILDTETDKVEKIRI
                     DIEE"
     gene            complement(7814..9730)
                     /gene="metG"
                     /locus_tag="PLV81_10065"
     CDS             complement(7814..9730)
                     /gene="metG"
                     /locus_tag="PLV81_10065"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018123220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOM10481.1"
                     /translation="MKKRFYVTTPIYYVNAEPHIGHAYTTIVADFVNRLYKMRGYETF
                     FLTGTDEHGDKIVKAAMQNNANPQEYTDKISAIFKKQWQSMGLEFNDFIRTTEERHKK
                     VVQYVLQKVYDNGDIYFGSYGGFYCFGCERYFTEKEMVDGKCPDHQKPLEFIKEDNYF
                     FKMSKYQQWLIDYINEHPDFIRPERYKNEVLAMLEGEALEDLCISRPRARLSWGIPLP
                     FDNNFVTYVWFDALINYISAIGYPDSDLFKKFWPVSNHIIAKDIVKPHGVFWPTMLKA
                     AGIEPYQHLNVHGYWNMEAAKMSKSLGNVVTPSQLLEKYGNDEIRYFFLREMTFGLDA
                     KFSEEAIVNRINYDLANDLGNLINRTFNMVHKYCDAKVPLFHDDSPAGKTDMQKALDD
                     AMNHYFDYIDAFQFNLALERLWDFIRYCNKYIDEHKPWQLAKEGNTRQLQSLLRNLLE
                     GIYAIAYLLAPVCITKSPVIINALKAPVQYPVEQLVKLSALTEGSIIDNPGILFPRLE
                     KENDRAPQKTKKEEKPMEELEGLIDIKDFMKVELRIAKVLQAQAVEGSSKLIELQIDT
                     GKDTRTIVAGLAPHYTPEYLVGKKIIVVANLKPATLFKRTSHGMLLAAKLSKDDKPIL
                     IEVNDTVPVGAVLS"
     gene            complement(9734..10597)
                     /gene="ricT"
                     /locus_tag="PLV81_10070"
     CDS             complement(9734..10597)
                     /gene="ricT"
                     /locus_tag="PLV81_10070"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOM10482.1"
                     /translation="MDITGVKLRCSQQVYYVYTNNLFVRRNSLCVVETEHGIDIGRVF
                     KKLTSLPDESIKVGGKLLRLATQDDLNLLPQIEDLEDRAFAVCKEKIIEKKLEMKLVM
                     VKSLFDKTKIIFYFVADGRIDFRELVRDLAAVFRTRIEMRQIGVRDEARMCGGYGTCG
                     RQLCCVNLKEEFEPVSIKMAKDQNMNLHSLKISGMCGRLLCCLGYEYHIYREINESLP
                     SPGFQFIVNDIPFVVEAIDSLKESLLLRHDSNTVAIHRDEISNMNGTFVLSDTAIEKL
                     KHSLEVVTEEV"
     gene            complement(10619..11551)
                     /locus_tag="PLV81_10075"
     CDS             complement(10619..11551)
                     /locus_tag="PLV81_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10483.1"
                     /translation="MTQILGNTVQQSIILHGAKNNILPHSLLFSGLEGIGKKLCAVKL
                     AKTMLCASGNACGACPSCKQIDHATHPDVIIIEPNEKGNIPIGSDEQEGSIRWLIGRV
                     NLASLSQKRVIIINDAHCMNIQSQNALLKTIEEPPAHTFIILISSERNQLLPTIHSRV
                     TEYVFHPLSDSDLVSILQKSGFDGQTCDSIIPVAGGSVSYALTLARNDLYSQLKEAYS
                     AIASFVCQPEVFSYDLSPILQEIGAYRTIDLLINIFRLHCTKNVVPWLHSALDRTHAI
                     EIIKILLELRKGISHNINIRMALKGMLYSLCFTK"
     gene            complement(11556..11999)
                     /locus_tag="PLV81_10080"
     CDS             complement(11556..11999)
                     /locus_tag="PLV81_10080"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF327 family protein"
                     /protein_id="HOM10484.1"
                     /translation="MIEIVPPNPKKEEQKIVKRKKGTKSSGLHSTFRSTFEGNLEFLL
                     PETLDELLNDLKDQEKKFMDDPSLYNMQRYKALVQRILKILVSEEYVVNTLQRKRRDR
                     ADFVIIQQIQNKLEEITKAYITNNKAFDILKTFDEIRGLILDLTH"
     gene            complement(12001..12360)
                     /locus_tag="PLV81_10085"
     CDS             complement(12001..12360)
                     /locus_tag="PLV81_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOM10485.1"
                     /translation="MARTNKFNIEDNIVNSIIGEGSEFKGEFNINGLLRIDGKFKGTI
                     ATDGKVLIGQTGEAITDIKARVVVIGGTVKGNIYATERVIMLSTGTIHGNVITPSLIM
                     EDGVIFEGNCTINKRQE"
     gene            12666..12809
                     /locus_tag="PLV81_10090"
     CDS             12666..12809
                     /locus_tag="PLV81_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10486.1"
                     /translation="MRKTILKLLEYGWPDRIIASYLMIKPAIVKYYRRKYRISRNEYQ
                     GFY"
     gene            13394..13651
                     /locus_tag="PLV81_10095"
     CDS             13394..13651
                     /locus_tag="PLV81_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10487.1"
                     /translation="MKDKCQYADKCPIFTGILQDMVITTKVFKNQYCEAGIEGRNKCK
                     RFQCMQKYGYVPDTVLPHSVQTLEDIAKEYQLHKTHIQNLK"
     gene            13704..13775
                     /locus_tag="PLV81_10100"
     tRNA            13704..13775
                     /locus_tag="PLV81_10100"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13737..13739,aa:Arg,seq:ccg)
     gene            14031..14942
                     /locus_tag="PLV81_10105"
     CDS             14031..14942
                     /locus_tag="PLV81_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012066347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HOM10488.1"
                     /translation="MRKLTHIIEKYFLVWVVLLSLCGYHFPSAFMPLVNYIPLFLGII
                     MFGMGITLKPSDFIVVVKFPLALVTGVVAQFIIMPLVALCLCMLFNLPPLLAIGVILV
                     GTCPGGTASNVITYLSKGDVALSVAMTTVSTLLSPALTPLLTYLLAGRWVPVPIVSMV
                     ISIVQVIIVPVALGIMVRLLLKKYIDRVLMVLPSVSVVAIVTIIAGITAANAHALRTV
                     GFITVLVVILHNVTGLALGYAAASLLGFDKSVRKTIAIEVGMQNSGLAVSLAIMHFEA
                     LAALPGAIFSIWHNISGSIAAWWWRRR"
     gene            complement(14910..16526)
                     /locus_tag="PLV81_10110"
     CDS             complement(14910..16526)
                     /locus_tag="PLV81_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOM10489.1"
                     /translation="MNAESILQKIRHIVFPERDEFPPSYFGELNYQCSRVIFPASLVC
                     IFAWLNYVGVDRQICPNEPLIPSLRYGLTVVSLIIFILQFFKFFKRHSMALLAILGLY
                     LESATGIITGLAKGDPVYMGGYFFVLMIPLVAPMKKIILWSEILFSVALFFIVGLSHG
                     MEFNTVRDQYKLNDMIVVVLFTLGFIYILDRLRYISWQKSQQIEEQRLHIQFEKERAE
                     SIVAEAKTVVEHVRKASTIINNFSGDITAIIHEHSAIFEKSRAISTDIIESFTVLKEA
                     TLKEIENNKEINDITKGLGTDLTNAAETGKQASGEAKKIQELSDDCNRKLQDARQTIE
                     RLRDESKMIEEISQTINEISDQTNLLSLNAAIESARAGEHGRGFAVVADEISKLADKS
                     QSSAKEISGIIGRSVQRILSAAVQIDDASLALIEIIQFLEANRSFFLKFQELVSSQDK
                     NINTVIGHLGNSLKYTQYINELTEKNATAVEQLQQALEQIHTFYDNLTDISMTFKKLA
                     GELTIHVDNLQKTLGDNSINLPSSPPPCSN"
     assembly_gap    16763..16862
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16863..>18187
                     /gene="serA"
                     /locus_tag="PLV81_10115"
     CDS             <16863..>18187
                     /gene="serA"
                     /locus_tag="PLV81_10115"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOM10490.1"
                     /translation="EAATAKGIIVTNAPGGNTISTAEFALSLICSLARNIPQADASMK
                     KQLWEKKKFEGVELRRKVIGIIGLGRVGSELSKLCKALGMQVMGFDPYLGKEKLAAMD
                     IDIADLETIWKKADFISVHTPLTDETKNLINADVIKKLKPTVRIVNCARGGIVNEQDL
                     YKALKEKRIAGAALDVFTQEPPENLPPFHELDNVILTPHLGAYTYEAQVSVAIEAAQT
                     VIDYFTKGVIHDSINYPSVDMQQYDFLKPFINLAERMGKFQAIMLGGKVRSVQLYYSG
                     EFGKYSREPLTAAYLKGLLSPYIDRPINYVNATMIAAERGIKVHVGESTQSGDWAHLV
                     QVKAEGGTLTNELWGTVIAKQPWVVRCDEYLIDFIPSGKMLVMHNNDVPNVIGAIGMF
                     LGKNHINIANLHLARTRRGGKALVILEIDEDIPADVLEKLAELPEILDV"
     assembly_gap    18188..18287
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18288..18791
                     /locus_tag="PLV81_10120"
     CDS             <18288..18791
                     /locus_tag="PLV81_10120"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOM10491.1"
                     /translation="IIKSTTISIIVSLFFIFSAHIFLRFIGITPGAFMIAGGILLFVI
                     AMDMLLARPRRTQKLNDKEYEADTDIAVFPLAIPFLCGPGNIAALLMFSSQAQGDIVK
                     LAIISVISAVTFLIAMVVMALAGRLQQVLGQTGISVIHKLMGLILSAMAVQFIINGLV
                     QSNIIGR"
     gene            complement(18810..20603)
                     /gene="uvrC"
                     /locus_tag="PLV81_10125"
     CDS             complement(18810..20603)
                     /gene="uvrC"
                     /locus_tag="PLV81_10125"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOM10492.1"
                     /translation="MKNNLEQKIKNLPSKPGVYLFINQKKEIIYIGKASSLKKRVASY
                     FQKNLDAKTRVLVSHITDIDYIVTQSEIEALLLESSLIKKHKPTYNIQQKDDKRFPYI
                     AITLHEKFPRVIFTRKLFHNGDRYYGPYTDAHAARKTVALLNKTFKLKTCSRDIPLKP
                     HERPCLNYQMGQCQGICTGTVTEDEYRRVIDTVMQFLDGTIDPVLENLTALMNSYSQA
                     MRFESAAKVRDMIFDIQKMLQAQHVVTPLGQNQDYIGITTHKDEAYILVFQFRHGSLT
                     GKRIFVYNNAEYTSRSSLIAQFIVEYYATNEPPQTIVIPDMIEDRTIIEEYLSSKTSQ
                     PVHTVTPRTDHEKAVMHMLHKNLDLAIAQKIAPGWQKALFELQEVLQLPALPMDIECF
                     DISNIQGNFAVASMVHFTDGMPNKKEYRRFRIRAYTKPDDPGMIHEVVGRRLQHLLNE
                     GLPLPDLLVIDGGKGQLSRAREAMETLECSVPIIALAKRLEEIYLPQSSDPLQLPKTS
                     PALKLLQAIRDEAHRFAITYHRNLRDKTMTASVLDRISGVGPQYKKKLLQSFDTIEHI
                     KQSDAQTIAQIAKIPATVAQKIYDFFHKGES"
     gene            complement(20617..>21605)
                     /locus_tag="PLV81_10130"
     CDS             complement(20617..>21605)
                     /locus_tag="PLV81_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM10493.1"
                     /translation="ITTQTISQIYTTSLDYAHRATIDHVESMSMVLYNFLNKVNTDNK
                     TTEHALFMPVKNNISVYTAYIVKPTDDDRNYTITTVIKGKPQIKNLTRVDTSQEFLIR
                     KGFAHVTINPVIEKQGTITYMESYIPLHGNSKPQCLIIQWLTPNITILQQKYDALIKT
                     LMQVIAIVIVATIIIIIIISMIHSYLTKSLIQELSHNIQSIATGSLDVTLNDSIDSEL
                     KPIATSFNSLVEKIKHTDQMLHTVKEETLSDIFKKGVVLLKDGKLDEALACFTVTLLY
                     KPHSFASYFNAGVCLAKKEKFNDALQYFEKARELNPDNELVLRYIGKIKAVQ"
BASE COUNT         6235 a         3981 c         4440 g         6749 t
ORIGIN      
        1 atactggcgc tgcagtagga attacagcag ggtttatctg gtaatagcag tgagtggggg
       61 ttttagcaaa cccccatttt gtatatactg gcgctgcagt aggaattaca gcagggttta
      121 tctggtaata gcagtgagtg ggggttttag caaaccccca ttttgtatat actggcgctg
      181 cagtaggaat tacagcaggg tttatctggt aatagcagtg agtgggggtt ttagcaaacc
      241 cccattttgt atatactggc gctgcagtag gaattacagc agggtttatc tggtaataaa
      301 aagtgaaagt gcggggtaac caccttcatt atttttatgg tgaaaattga aagcttttag
      361 catctgagtg ctgcataacg ttattgtttt gtgcagatat attttataat catttcattc
      421 ttatttaatc ttgcatcaat tttataacca gcattgtgca acatgtgttc aatagcccag
      481 tcaaaggtac tgaattcttc tttgacatgc atttctactt ctctggcaaa ttcatccccg
      541 gtttgttgtt taaattgatt aatccactga tcaatgtttt gttctattgt ttctatatca
      601 cctgaaaaga ttacatcctc atacaataat ctgccgccag gttttgtaat tgatgaaata
      661 tttttcaacg ccatctgctt ccagaaatcc ggcaggtggt gtaatgccat ttttgtatac
      721 acagcatcaa caataaaggt atgagtatgt aagcctaaaa atcctgagtg aatatactct
      781 atattgtgta tagtatggta tttttgccgg gcgtaatcaa gcattggact ggatatatca
      841 atggcatata ttttttttac tttttttgct atatattgag taatggcgca tgtgccacat
      901 ccaatatctg caacaatgct ttgagtggta agtttcaaat cactgattat ttcatctgct
      961 tcttttgcaa agtttctgaa tttagagtga ttttcatcat actgctgtat gagctcaatt
     1021 gaggcgtaat taacaccaga atgtttagat tcgtcataaa accagctttt cataattaca
     1081 ttgtttcctt tataaaatat ttttgtgatt ataaatgtgc aatcagatta tctatgtcac
     1141 caactatttc aaatcctgca tggctaccgg tatcgtccag aacagtatgg ttattttcgt
     1201 taaatacaca gtgaacatgc gcatctaaac tttcatatat tttggattgc atggatccgt
     1261 tgtatggtgc tggtaaagct atgccaggtg ttcttttaat tgttatggta gcgttacggt
     1321 tgcccttact gcagctaatg cgtaccagca aatctgtaac tgatagggta cactgcgcat
     1381 tattatatgt ggtaaatgta tatagtctgc cattgtgcag taatgcaata agaaaaccgg
     1441 taaagtatga tcgtttccat ggtattgttg caatggagcc aaatatactg gttcctactg
     1501 tagcaaaatg atttgtctga aaccatatat aggcactggg aaaagattta ccccagtctt
     1561 tttccatgta accgcgccca cctgtaaagt ctatgagttc ttcattatag cggatagtac
     1621 cactgattga atgatccata ctcaacacag tatggttgca ttccatagtg ggaacaaatg
     1681 cataccaccc catagaacct ggtgaaaaaa gttttactgg ccacgggtag cgattttcaa
     1741 agctacattg tcctgaaaca gaaatgtcac tggacgtgat ggcaagtgtt atagtattga
     1801 gtgtgaaatg atttcctgca atgctaatcg aaaaaggggt atacgttgca gtaaactcat
     1861 gaaatggata tgttacaaaa tgaggggatt ggcctttttt aaaaatatgt atgaacgcat
     1921 acgattttgt tttgtctttt tctatgaata tgccaggtat cacaataaag ctgtgttttc
     1981 cgtctttatc cacttgcttg taataccagc cttcaaaata gggaccttta gcgggattat
     2041 gataccattc gggatgccag atagttttta aagtatgaag catagtgtta gaattataat
     2101 actgcataaa ccaataatca agaaaaaata aggggtcaga gcattgatgc ctcttcataa
     2161 ctaaacaggt aattaatctt gacacaacaa cacttgcatt ttagtattag cgatagttca
     2221 taaaccaaga ggactatgca aacagatatc aaagcactaa aacgaaaagc aaaagctctg
     2281 cttttgtcaa aggacatccc tgccggaaaa atggatattg taagggcaat acttgccaat
     2341 gaccgtatac ttgaggaaga aaaatacaat gccatcatag atctggttaa aaactatccc
     2401 gacaagcctg taaagccggt ggccccagga aaagttgaag tgaaagggga agagtttaaa
     2461 acgcctgagg aatataactc aaagaacacc gcctatctgt accagaagta caggcatacc
     2521 aggcttttta aaaaaagact ccttgtccat gcaaataata aatttggtat tggttttttg
     2581 cggcgccttg ttccttcaca acgtctgtta aggctttccc agaaattatt tgaactgcac
     2641 tatgctatgc gtcagcgcct gtacagagta ctttgtgata ttgttgatga tcagtcaata
     2701 tctgacccta caatttttaa ttattgtgtt gtactgtaca atgcgcttgc atcgtcatgc
     2761 ttgattgata agcagtatat tgaacttaaa tggacacggc ctgaggggtt taagcagtta
     2821 ctatcgccct atatgaattc tatgctcaag ctttccctgt gcgatgctca gaccaaagaa
     2881 gcaatgattc aattaattga ccagaaattg cgtcaaagcc aggatcttca gaaaaatgaa
     2941 acaatgaata aaaagaatct gaccattgaa caatatattt atgatttcat caccacactg
     3001 cgatgtttgc tgtatccttc taccaccaat gaatgttcac tgactaacct tcttaaaaaa
     3061 gaaggatcgt tgccatcatt tgaagcattc tgtattgcct gtgttgaagc gctggttata
     3121 ggcaaacctg ttgtgttatc tgatatcatg actatgtttg atattactgc tcccaaggtt
     3181 tcggatactg aatggaattt tgatcacgat gaactggtaa aggcagggaa ggaccccgaa
     3241 tcgttaaaaa agaaagcaaa ggaagagctg caccgaaagc ttgagccgta cgatgaattg
     3301 tacaggtttc ttacctatgc acctgagggg aaggaagttg ccattcaggg ttttgatgat
     3361 cagcgtaaac tggtagataa gcgcaatgaa gtgtttgatg atgcatacag taaagacata
     3421 ctcattgcac ttgaagggat gcttctgggt tttatcaatg aatatggggc actgcttgat
     3481 ggtacaacga tatattttga aaacaataaa aatcactatg aaggctgtat atttactgaa
     3541 aattattttt cacatgaatt agcacgtttt tctgagatac tttcagaaat caatacattc
     3601 aggtccaaca atccaacatt tcatgtaacc cgtgatgaat tgatagaagg tataaataaa
     3661 aagaaaaaaa gcctgtaccc catagaacgc tttatgaccg tgattggcga tatgtgttat
     3721 gatattggca ttgaattatt taccctgtat aatcgtcatc gccggtgggt tttgagtggt
     3781 tcaccgggca aagacgatgc tgtgatgcgc acggcatttt ctgcacatac atcagggtta
     3841 tcatcacttg atgacaggga agcaatgcct gtcccctttt ccgattgtat cataaccggt
     3901 ttttcaaaat tgcggccact gtcaaaactg ttgctacaca agccggtcat acatcccaca
     3961 ctgcgtgatg gagtgttccc tcagatggtt gcttatctcc tgcagatggc acatgtatgc
     4021 ctcaatgaca ggctatatgg tatactggat gaacgtacaa aaatattatc gcaaatacgg
     4081 agtctttcat gaggctttta gtaatccttg ccatgctttc ttttgcattg cgcgctgagg
     4141 cagcaccacg tgttctcgtg ttcaaatcgt atcatgacaa cctcacttca ttacttgatg
     4201 aatatcgtat tccgtatatt gtaaaacggg catcggtagt ggcgggcgat agttccctgt
     4261 cagggtttga tgctgtgttt ataccctgcg gtgttgagca gcctattgag gcaagcattc
     4321 aggtaatggc acatgggcaa cgtattcagg gtgttgcatt aaaagatgat tatgaaggta
     4381 tagattttaa gcgattgaat gcactgcttc aggattttgt gaaaagcggt ggtaggttat
     4441 atgctgcagg ttattcatac agatttgttg aagcattagg atgttctcta tcctttttta
     4501 ataattttcc ccattatggc accagcggca ggatacatag ctttttaaat gatgattttg
     4561 cttcatttgt tatgcgtgat gacatgcaac ttactgtcaa ctataatggg tgggtggcat
     4621 tgcaaagagc caatgcatcc atactggcca aagggtattt ccccactgtc ggtggtgagc
     4681 gggaagggcc tgttgctttt tttaaaaaat atggtaatgg ggttgtgtat tattcagcgt
     4741 accataccgg cgaggactcg ttgtatatgc gctattttat tttcagaaca gcgtatggca
     4801 tgcttgttga tgagctttta cagaaagcgt ggtactgggg tcagagcata gcattatcgg
     4861 tggttgacac gtttaagggt gacgaatatg tcagaagtta tacctgcacg ctacctcagg
     4921 gtgctattac aatatatctt ccaaccacgt acccggtaca ggttgatatt tttgccaaca
     4981 aaaaactttt aggttcatgg gacagtggat ttataactga aatatcattg tataaccctg
     5041 caacacagga tgctacagta tatgtatatc agagtaacag agcagcgtat aaaccctttg
     5101 cactggttat agcacagggt aggcgtatac tgccgtatat gccagtagtg cgccttagca
     5161 tgggtgctat tgctgtcatt atgcttttag gatttacatg gaagaggata aatccccgaa
     5221 agtttgccgg taaacgccgc tactggttca gggatgggca ttttattcat aggcaaaaat
     5281 aaataggtat ttatattccc ccacaacctg tggtgtgagt atatagtaaa aaaagcttgc
     5341 aattgaaggt atacaattca tacatatgta tacatttaac gataacatac atatcatagc
     5401 agggggctgt cccaaaagtc gtttcgatac aaatttcgca acgctcattg agtaattttg
     5461 ccagattttg tggcaaaatt gtatcgaaat gagcgagtat tacgatataa tgtttgcaaa
     5521 atttactcaa cgacctcttt tgggacagcc ccatatcact attttgctta gccatatgta
     5581 gtgattgtta caggttttag cctgtacata ttgggaaaat ttttcaaagg gagctgtcaa
     5641 tgggtaaaat tatatccatt tccaaccaga aaggaggggt tggcaaaaca acaaccgctg
     5701 tcaatttagc tgcattttta gcagaaaagg gtaaacaggt tctggttgta gatattgacc
     5761 cccaggcaaa cgcgggattt ggcttaggtg tcaatgttga ggaaatggaa acaacagtgt
     5821 atgaggtatt gctgggtacc attcccgtac aggaagcaat cttcaggaca actatcgaaa
     5881 atctttatat agttccatcc aatatgcatt tagccggggc tcaagttgaa ctcatggata
     5941 tggaggataa agagtattta ttgaaaaggg cgctggaaaa ggtacgcgat acatttgatt
     6001 ttatacttat cgactgtcca ccatcattag gaatactcac gttaaatagc ctggttgcat
     6061 cagattcggt tattattcca ctgcagtgtg aatactatgc acttgagggt ttaagccagc
     6121 ttctgaaaat tatttccatg gtacaggata cactcaatcc aaagcttgaa attgaagggg
     6181 tgctactgac catgtatgac ccgcgaacta atctttcgca gcaggtggtt tctgatgtaa
     6241 aacaattttt taagaataaa gtgttttcag caataatccc acgcaatgtg cggctttctg
     6301 aggcaccgtc gtttggcaag cccatcaatc tttatgacag gacttccagt ggcagcatga
     6361 gctatgagca actggcacat gaactaattc aaaatgtgcg atagctcaca ggatacctga
     6421 caggagcaga taatggcaaa aaaagtttta ggtaaaggat tgggagcaat catcaccacg
     6481 tcaccaacac cggtgagtga ttttgaaaag gttctgctac aggataaaga acgtattgta
     6541 gatattccta tcaatgcaat aatgccaaat cctgatcagc cacgtgtcca ttttgatgca
     6601 gcagagatag cggggcttgc acaatccatt cagcatgtgg ggttgctgca gccaattatt
     6661 gtgcgcaagc agggtgagga atattttatt gtagccggtg aacgcaggtg gcgagcatgc
     6721 aagttattgg gaaagccaac catcaaagcc attgtcatga acacaaccga agagcagaat
     6781 ctgacactgg ccctcataga gaacattcaa cgcaccaact tagatcccat agaagaagca
     6841 aaagcatata aaatgcttgc aacacgcttc aaactcaagc aatcagaaat agctgatcgt
     6901 gtgggcaaag atagggcaac ggttgccaat atcatgcgct tgcttaatct tccggagcac
     6961 atgcaggaag cattgcagga tgggaggatt aatgttgggc atgcaaaact gcttttaggt
     7021 gtttctggtg atgagcagga ggcattgttt gacaggataa tccatcaggg gctttcagtg
     7081 cgtgacgtag aaaaagcaat agccgaggtg ccaaaaactt ctggcaaaaa gaaaaagaaa
     7141 gcaaaggatg cacatatacg gaaactggaa gaagagctta tctcatattt aggtactcgt
     7201 gttgaaatac ggcattcagc ggggaagggt cgtattgaaa taagttttta ttcgcttgat
     7261 gattttgaac gcatagtaag tcttataaaa ggtgacaggt agttattctt ctatatctat
     7321 acgaattttt tctactttat cagtttcagt atcaagaatg gcaacagtat gttcctcacc
     7381 gcggtagagt gaccctggat tgataatacg tgtttttccg ctgacataat tttcaaagat
     7441 atgggtatgt cctttgatga tgtaatcgta tgcaccactg ttaacagctt ccctgaacat
     7501 gggaacattg ttaccatgaa ataccatgaa atgtttgcca tccagcgtga actgacatga
     7561 atgttcaacg catccaaatc cgcattcaga tgcagttgta tttatttctt caacatcaat
     7621 atcacagtta cccagcacaa atctgccatc aaagccttta aacagagaaa taatttttga
     7681 tgaagtaagg tcaccagcat ggataataac ggatatattg cgttctttga atatagcaat
     7741 tgccttctga accagttcaa tatcattgtg tgtatcggat attaatccaa tcttcataaa
     7801 gcctcatggt gtattaactg agtactgcac caaccggcac cgtatcatta acttcaatca
     7861 gaatgggttt gtcatctttt gacaattttg ctgccagaag catcccgtgt gatgttcgtt
     7921 taaacaatgt ggctggttta agatttgcca caacaatgat ctttttccca acaagatatt
     7981 caggagtata atgaggcgca agccccgcaa caatggtgcg agtatccttg ccggtatcta
     8041 tctgcaactc aatgagttta cttgatcctt ctaccgcctg tgcctgcaat acctttgcaa
     8101 tacggagttc aactttcata aaatccttga tgtcaataag gccttccaat tcttccattg
     8161 gtttttcctc ttttttagtt ttttgtggtg ctctgtcatt ttctttttct aatcgcggga
     8221 acagaattcc cggattatca attatgcttc cttcagtaag tgctgatagc tttacaagtt
     8281 gttcaaccgg atactgtacg ggagctttaa gtgcattaat gatgacaggc gatttggtga
     8341 tgcacaccgg tgcaaggagg tatgcaattg catatattcc ttctaacagg tttctcagaa
     8401 gtgactgaag ctgcctggta ttgccctctt tggctaactg ccatggtttg tgttcatcaa
     8461 tgtatttatt gcagtagcgt ataaaatccc acaggcgctc aagcgccaga ttaaactgga
     8521 aggcatcaat ataatcaaaa tagtggttca tggcatcatc aagtgccttt tgcatgtctg
     8581 tttttcctgc ggggctgtcg tcatgaaata gtggtacttt agcatcgcag tacttgtgta
     8641 ccatattaaa ggtacggttt attaaattac ctaaatcatt tgcaaggtca tagttaatac
     8701 gattaactat cgcctcttct gaaaatttag catcaagccc aaatgtcatc tcccgtaaaa
     8761 agaagtatcg gatttcatcg ttgccatatt tttctaatag ctgtgatggt gttaccacgt
     8821 tgcccaatga ctttgacatt tttgcagctt ccatattcca gtagccatgg acattgaggt
     8881 gttgatacgg ctcaatgcct gcagctttca gcatcgttgg ccagaaaaca ccatgtggtt
     8941 taacaatatc ctttgcaata atatgattgg atacaggcca gaatttttta aacaggtcac
     9001 tatcgggata tccaatagcg ctgatataat taatgagcgc gtcaaaccac acatacgtta
     9061 caaagttgtt atcaaagggt agcggtatgc cccaggaaag tctggccctg gggcgagaaa
     9121 tgcataaatc ctcaagcgcc tcaccttcaa gcatagcaag tacttcattt ttatagcgct
     9181 cgggccgtat aaagtcggga tgttcattaa tataatcaat cagccattgc tgatatttgc
     9241 tcatcttaaa aaagtaattg tcttctttaa tgaattccag aggtttttga tggtcagggc
     9301 atttgccatc aaccatctct ttttcggtaa aatagcgctc acagccaaag cagtaaaaac
     9361 caccgtagct tccaaagtag atatcgccgt tatcatacac tttttgcaac acatactgta
     9421 ccactttttt atggcgctct tcggtggtgc ggataaaatc gttaaactcc aggcccattg
     9481 actgccactg tttcttgaag atggcactta ttttatcagt gtattcctgg gggttggcat
     9541 tgttctgcat ggcagctttg actatcttgt caccatgttc atcagtgccg gttaaaaaga
     9601 aggtttcata gccacgcatt ttatacaggc gattgacaaa atcagccaca atggtggtat
     9661 aggcatggcc tatatgtggt tcagcattaa cgtagtaaat gggcgtggta acataaaagc
     9721 gctttttcat tgattatacc tcttcagtaa caacttccag tgaatgtttt aatttttcaa
     9781 ttgcggtatc gctcaacaca aatgttccat tcatattgct aatttcatcc ctgtgaatgg
     9841 caacagtatt ggagtcatgc ctcagtagca atgattcttt taagctgtcg atagcttcca
     9901 ccacaaatgg gatgtcatta acgataaact gaaaacccgg gcttggtagt gattcattaa
     9961 tctcacgata aatatggtat tcatatccaa ggcagcatag taatcttccg cacatgccgg
    10021 aaattttcag agagtgcaaa ttcatattct ggtcttttgc cattttgatg gaaaccggtt
    10081 caaattcttc tttcaggtta acacagcaaa gctgcctgcc acaggttcca taccctccgc
    10141 acatgcgcgc ttcatcacgc acgccaatct ggcgcatctc aatgcgtgtc ctgaacaccg
    10201 cagctaaatc ccgcaccagt tccctgaagt caatacgccc atcagccaca aaatagaaga
    10261 taatcttggt tttatcaaaa aggcttttta ccatgacaag tttcatctct aatttttttt
    10321 caatgatttt ttccttgcac accgcaaaag ccctgtcttc aagatcctca atctgaggca
    10381 ataggtttaa atcatcctgt gtggcaaggc gtaaaagctt cccccccacc tttattgatt
    10441 catcgggaag ggaagtaagt ttcttaaaaa ccctgccaat atctatgcca tgttccgttt
    10501 ccacaacaca cagggaatta cgacgaacaa aaagattgtt tgtatataca taataaacct
    10561 gctggctgca tctgagtttt acacctgtta tatccatagg aaaacctgca caaaaaaatt
    10621 atttagtaaa acataaagag tatagcatac ctttaagtgc cattctaata ttaatattgt
    10681 gggaaatacc ctttcgcagt tcaagtaata ttttaataat ttctatagca tgcgtccggt
    10741 caagtgcgct atgtagccat ggcaccacgt tttttgtgca gtgcaatctg aatatattaa
    10801 tcaaaaggtc aatagttctg tatgcaccaa tttcctgcag tatgggcgat agatcatagg
    10861 aaaaaacttc aggctggcaa acaaagctgg caatagcact gtatgcttct ttaagctgtg
    10921 aatagagatc attacgagca agggtcagag cataggagac agaccctcca gcaacaggga
    10981 tgatggaatc acatgtttgc ccgtcaaagc cacttttttg caggatggat acgaggtcgc
    11041 tatcgcttag aggatgaaac acatattccg taacacggga atgtatggtt ggcagcagtt
    11101 gattgcgttc agagctaata aggataataa acgtatgggc tggtggttct tcaatggttt
    11161 taagcaatgc attttgcgac tgaatgttca tgcaatgagc atcattgatg atgataactc
    11221 gtttttggga aagggaagcc agattcacgc gaccaataag ccatcgtatg cttccttcct
    11281 gttcatcgct gccgatggga atgttccctt tttcattggg ttcaatgatg ataacgtcag
    11341 ggtgtgtagc atggtctatc tgcttgcatg aaggacaggc cccgcaagcg ttgccactgg
    11401 cacacagcat ggttttggca agtttaactg cacagagttt tttaccaata ccttccagcc
    11461 ctgaaaatag caaagaatgg gggaggatgt tgtttttggc accatgcaaa ataatagatt
    11521 gctgaacagt attgcctaaa atttgtgtca tggttttagt gggtaaggtc caggattaat
    11581 cctctgatct catcaaaggt ttttagtatg tcaaaagcct tgttgtttgt tatataggct
    11641 tttgttatct cttcaagttt attctgtatt tgctgaataa tcacaaaatc agcacggtca
    11701 cggcgttttc gttgtaatgt gtttaccaca tattcttcac tcacaaggat tttcagtata
    11761 cgctgaacca gggccttgta ccgctgcatg ttgtacaatg acggatcgtc cataaatttt
    11821 ttttcctggt ctttcaggtc attgagaagt tcatctaagg tttcaggcaa caaaaattca
    11881 agatttccct caaaggttga cctgaatgtt gaatgcaggc ctgaggattt tgtgcctttt
    11941 ttgcgtttaa cgattttttg ttcttctttc ttggggtttg ggggtactat ctcaatcatg
    12001 tcattcctgc ctcttgttta tagtgcagtt accctcaaaa atgacaccat cttccatgat
    12061 aaggcttggc gttatgacat tgccatgaat ggtcccagtt gacagcataa taacccgttc
    12121 agtggcataa atattgcctt tcacagtgcc gcctatcacc accacgcgtg cctttatgtc
    12181 ggtaatggct tcgcctgtct gtccaataag caccttccca tcagtggcga tagttccctt
    12241 gaatttgcca tctatacgca gcaagccatt gatgttaaat tcgcctttga attcggaacc
    12301 ttcaccaatg atactgttaa caatattatc ttcgatatta aatttatttg ttcgtgccat
    12361 attctcatta taatattaat ataatattta ttttttagga attactatct tcatgcagga
    12421 tacaatacaa aataacaggt taaaaaacaa gtattattta ttatgaaata atgaacatcc
    12481 atgacacaag aaaaggtggg atgttgatta cagtacccat tctaatgtga cataaaattt
    12541 ttgtgccaaa attattatta tcggaaaaaa ttttaaaaat tttactaaaa aaagcttgca
    12601 aaatttataa ttacttataa tataaatcca acggaatagt ctgcccaaaa atcaaggagg
    12661 aaagcatgag aaaaacaatt ttaaaactcc tggaatatgg ttggccggac aggattattg
    12721 ccagctacct tatgatcaag cctgccattg tcaagtatta tagacgaaag taccgcattt
    12781 cccgcaacga ataccaggga ttttattaat attctttgat ctttttacat aactattaat
    12841 aaaaaaagta gaggggaaga gggttcccct ctaaagagaa tttggaggtt cgccaatcat
    12901 taaattggag gaaagtatag caccaaaaaa aattttgttg cttacttgtc aagaatattt
    12961 atagcttata tcggcaaaaa aacaaaaaaa ttttgtatta atcctgtaaa aaatcccttt
    13021 attcatgtct atataatgta tagtatatat tattttttta ttgtaaagtt ttatgctcta
    13081 agctttttaa taattttttt tgcacgatgg aaagtgactt cattctgctt gctatacatt
    13141 acatattgcc tgtgtaatcc acacactgcg tataatatcg cgaaattctt tgtacgaaaa
    13201 gaaggaaggg tacgtatatg agttttaaaa gttaaaaaat ttttttagat gttatttgta
    13261 ttgtatacaa aagagggttt tgtatttcaa ttttttataa ttgaataatc tgtaaatagg
    13321 tgatattaat aaaacagtac acgaatcatt gttgtaatga atgttgtata aagaaatatt
    13381 caggattatg accatgaagg ataaatgtca atatgcagat aaatgcccca tttttactgg
    13441 cattttacag gatatggtta taaccacaaa agtatttaaa aatcaatatt gtgaagctgg
    13501 tattgaaggc agaaataagt gcaaacgatt tcaatgtatg cagaaatatg gttatgtgcc
    13561 agatacagta ttgcctcatt cagttcaaac tcttgaagat attgcaaaag aatatcaatt
    13621 gcataaaact catatacaga atctcaaata atcttgacag gtatcaatct cttctgctat
    13681 atatgcctat atatcgtata caggctctcc tagctcaatg gatagagcgt cggcctccgg
    13741 agccgtgtgt ctgggttcga ttcccgggga gagcgcatac ttgttttcta tgtatatgat
    13801 tgcataaaca aaatagaaag cagtggcaca aaaaatttta tgagggggtg tcccaaaagc
    13861 cgtttcgata caaatttcgc aacgttcatt gagtaatttt accagatttt gtggcaaaat
    13921 tgtatagaaa tgagcgagta ttacgatata atgtttgcaa aatttactca acgacctctt
    13981 ttgggacacc cccggtaact atcgcccctt ttttactatg gtatacctgt atgcgtaaac
    14041 ttactcacat catcgaaaaa tattttttag tatgggttgt tcttttatct ttatgcgggt
    14101 atcatttccc atctgccttt atgcctctgg taaactacat accgcttttt ttaggaatca
    14161 tcatgtttgg gatgggcata acccttaagc cttccgactt tattgtggtg gtaaaattcc
    14221 cgctggcgct tgtcaccggt gttgttgcac agtttattat catgccgctt gttgctttgt
    14281 gtttgtgcat gctttttaac ctgccgccac tacttgctat tggtgtgatt ctggttggca
    14341 cctgcccggg aggaactgcc tccaatgtta ttacgtatct gtcaaagggc gatgtagcgc
    14401 tttcggtagc aatgaccact gtaagcacct tgctgtcacc tgctttgacc cctttgttaa
    14461 cgtacctgct tgccgggcgg tgggtgccgg taccgattgt ttccatggtt atttctattg
    14521 tacaggttat tatcgtgccg gtagcactgg gcattatggt gcggcttctt ttgaaaaaat
    14581 atattgaccg tgtgttaatg gtgctccctt cagtttcagt tgtggcgata gttactatta
    14641 tagccgggat cactgctgca aatgctcatg cgttgcgcac ggtgggtttt attaccgtgc
    14701 ttgtggtaat actgcataat gtgacaggat tagccctggg gtatgctgct gcttcgttgc
    14761 ttggatttga taaaagtgtg cgaaaaacca ttgctattga agtgggtatg cagaattcag
    14821 gccttgcagt gtcacttgca atcatgcatt ttgaagctct ggctgcattg cctggtgcaa
    14881 tcttcagtat atggcacaac atttcgggtt caattgctgc atggtggtgg cgaagacggt
    14941 agatttatgg agttgtctcc cagggtcttt tgcaagttgt ccacatgtat ggtaagctca
    15001 ccagcaagct ttttaaatgt cattgatata tcagttaaat tatcataaaa agtatgaatc
    15061 tgttctaaag cttgctgtaa ttgttctact gctgttgcat ttttttctgt aagttcatta
    15121 atatactggg tgtattttaa agaatttcct aaatgcccaa tgacggtgtt aatattttta
    15181 tcctgtgatg atacaagttc ttgaaacttt agaaaaaatg acctgtttgc ttcaagaaat
    15241 tgaatgattt caatcagcgc taacgaagca tcatctatct gaactgccgc actgagaatt
    15301 cgctgtactg aacggccaat gatgccgctt atttctttgg ccgaagactg gcttttatct
    15361 gctaattttg agatttcatc ggcaactaca gcaaatcccc gcccatgttc accagcacgt
    15421 gctgattcaa ttgctgcgtt aagtgaaagc aggtttgtct gatctgaaat ttcgttgatg
    15481 gtttgtgata tttcttctat cattttagat tcgtcacgga gacgttcaat tgtttgacgg
    15541 gcatcctgca gtttgcggtt acagtcatcg gaaagttcct gaatcttttt tgcttcacca
    15601 cttgcttgct tcccggtttc agcggcgttt gtaaggtctg tgcccagacc tttagtgatg
    15661 tcatttattt ctttattatt ttcaatttct ttcaacgtag cttctttcaa tacagtaaat
    15721 gattcaataa tatctgtact gatagcacgg cttttttcaa agattgcaga atgttcatgt
    15781 atgatagcag taatatctcc tgaaaaatta tttataatgg ttgaagcttt acgaacgtgt
    15841 tccactactg tttttgcttc tgctacgatg ctttcggcac gttctttttc aaactgtatg
    15901 tgcaagcgct gttcttctat ctgctgggat ttctgccagc tgatatatcg caaccgatcc
    15961 agaatataga taaaccctaa tgtaaagagc accacaacta tcatatcatt gagtttgtac
    16021 tgatcacgta ctgtgttaaa ttccattcca tgtgaaagtc ccacaataaa aaacagtgct
    16081 acagaaaaaa gaatttctga ccatagtata atttttttca ttggagcaac cagtgggatc
    16141 atgagtacaa agaaataccc tcccatatat accgggtcac cttttgcaag ccctgtaata
    16201 atgccagttg cggattctaa atacagtcct aagatagcaa ggagggccat actgtggcgt
    16261 ttaaaaaatt tgaaaaactg cagtatgaat ataataagcg aaactactgt aagcccatac
    16321 cgaagagaag gtataagggg ttcattgggg caaatctgcc tatcaacgcc aacatagtta
    16381 agccaggcaa atatgcaaac taacgatgca ggaaatataa ctctgctgca ctgataattt
    16441 aattcaccga aataggatgg agggaattca tcgcgttcag ggaatacaat gtgcctgatt
    16501 ttttgtaata tagattcagc attcattatg actaaaacaa aaagaatgat agcggttttg
    16561 tcaaatataa ttatttttaa tgtaaaaaaa tggtgatatt agactttatg gtgcagaaaa
    16621 ggtaatacaa taaggccaga cgattacata attgccgcag cagtagttgt ttaacatggt
    16681 atacttatga cggaaaatat acaagaaata aaagaatttt gaaaaatcaa aatctaaggg
    16741 ttgaagactg gactgtgtaa tannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16861 nngaagctgc aacagcaaaa ggaatcattg taaccaatgc tccaggtgga aataccattt
    16921 caacagcaga gtttgctctg tcacttatat gttcgcttgc tcgcaatata ccacaggcgg
    16981 atgcatcaat gaaaaagcaa ctatgggaaa agaaaaaatt tgaaggggtt gaactgagac
    17041 gtaaagttat aggcattatt ggcttaggac gggtagggtc agagctatct aaattatgca
    17101 aggctttggg gatgcaggta atgggatttg atccttacct tggaaaagaa aaattagcag
    17161 ctatggatat agacattgcc gatttagaaa caatatggaa aaaagcagac tttatcagcg
    17221 ttcatacacc attaaccgat gaaactaaaa acttgattaa tgctgatgtt ataaaaaaat
    17281 taaaaccaac agtgcggatt gtaaactgtg cgcgtggtgg cattgttaat gaacaggact
    17341 tatacaaagc attgaaagaa aaacggatag caggtgcagc attagatgta ttcactcagg
    17401 aaccacctga aaatctgccg ccattccatg aacttgataa tgttatcctc acgcctcatt
    17461 taggtgcata tacgtatgaa gcacaggttt cggtagcaat tgaggcagca caaacagtaa
    17521 tcgattactt taccaagggt gttatccatg acagtatcaa ctatccatct gttgatatgc
    17581 agcagtatga tttcctcaag ccatttatta atcttgcaga gcgtatgggt aaatttcagg
    17641 caatcatgtt aggtggcaag gtgcgttctg tgcaactgta ttattcagga gagtttggta
    17701 aatacagcag ggaaccgtta acagcagcat accttaaagg gttactatcg ccctatattg
    17761 acaggcccat aaactatgtc aatgctacca tgatagcagc agaacgtggc attaaagtac
    17821 atgttggtga aagcacacaa tcaggtgatt gggcacatct tgtgcaggtt aaggctgaag
    17881 gtggcacgct taccaatgaa ttatggggga cggtcattgc aaagcagccg tgggtggtgc
    17941 gctgtgatga atatcttatt gattttatac cttcaggaaa aatgctggtt atgcacaata
    18001 atgatgttcc caatgtcatt ggtgcaatag gtatgttctt aggtaaaaat catattaata
    18061 ttgccaacct gcatctggca cggacacgcc gtggcggcaa agcactggta atactggaaa
    18121 tagatgagga tatccctgca gatgtgcttg aaaaacttgc agagctgcct gagattttag
    18181 atgtaaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatc atcaaatcca
    18301 caaccatctc aataattgtt tcactatttt tcattttcag tgcccacatt tttttgcgct
    18361 ttataggcat aacgcctggc gcatttatga ttgcaggagg aatactgtta tttgtcattg
    18421 ctatggacat gttacttgca cggccacggc ggacgcaaaa gcttaacgat aaagaatatg
    18481 aagctgacac tgatattgcc gtgttcccgc tggctatccc ctttttgtgt ggtccgggaa
    18541 atattgcagc actgcttatg ttcagttcgc aggcgcaggg tgatattgta aagcttgcca
    18601 taatatcagt tatttcagcg gttacattcc tcattgccat ggttgttatg gcacttgcag
    18661 gcaggcttca gcaggttctg gggcaaacgg ggatttcagt cattcataaa ttgatggggc
    18721 ttatcctttc ggctatggca gttcagttta ttattaacgg gttagtacaa agtaatatta
    18781 ttgggcgata gttactgtaa aaaagctttt taactttccc ccttgtgaaa aaaatcatag
    18841 attttttgag caactgttgc aggaatcttt gctatttgtg caatagtttg tgcatctgat
    18901 tgtttgatgt gttctattgt gtcaaagctt tgcaacagct tctttttata ttgaggacca
    18961 acaccgctta tacgatcaag gacagatgca gtcatggttt tatcacgaag gttgcggtga
    19021 taagttatag caaagcgatg agcttcatca cggatagcct gcagcagttt caatgcaggt
    19081 gatgtcttag gaagttgcag ggggtcagag ctttgaggca ggtagatttc ttctaatcgt
    19141 tttgcaagtg ctataattgg tacagagcat tctaacgttt ccattgcctc gcgtgcacga
    19201 gaaagctgtc ccttaccgcc atcaataaca aggaggtccg gcagtgggag cccttcattt
    19261 aaaaggtgtt gcagtcgcct tcccacaacc tcgtgaatca tgccaggatc gtctggctta
    19321 gtataggcgc gtatgcggaa gcggcgatat tcctttttgt ttggcatccc atcagtaaaa
    19381 tgcaccatag aggcaacagc aaaatttccc tgaatgtttg atatatcaaa gcattctata
    19441 tccattggga gtgctggaag ttgcaacact tcctgtaatt caaataaagc cttttgccag
    19501 cctggagcaa tcttttgagc tatcgccaga tctaaatttt tgtgaagcat gtgcataaca
    19561 gctttttcat ggtcagttct gggagtaacg gtatgtacag gctgtgaggt tttggagctt
    19621 aaatattctt caattatagt cctatcttca atcatgtcag gaataacgat ggtttgtggt
    19681 ggttcattgg tagcatagta ttcaacaata aattgggcaa taagtgaaga gcggctggtg
    19741 tactcggcat tattatacac aaagatacgt ttacccgtca ggctaccatg cctgaattga
    19801 aaaaccagaa tatacgcttc atccttatgt gtagtaatgc caatataatc ttgattttgc
    19861 cccaatggtg tgacgacgtg ctgtgcttgc agcatcttct gaatgtcaaa tatcatgtcg
    19921 cgcactttgg ctgcactttc aaaacgcatt gcctgtgaat atgaattcat aagtgccgta
    19981 agattttcaa gtaccgggtc aatagtacca tccaaaaact gcatcacagt atctattacc
    20041 ctgcggtatt catcttcagt aacagtacct gtgcatatgc cctgacattg tcccatttga
    20101 taattaaggc atggcctttc atgtggcttt aaggggatat ctctggagca tgtttttagc
    20161 ttaaaggttt tgttgagcag cgctactgtt ttgcgtgctg catgagcatc ggtgtagggt
    20221 ccatagtatc tgtcgccatt atggaaaagc ttgcgcgtaa aaattacccg cgggaatttt
    20281 tcatgcagtg taatggcaat gtatgggaaa cgcttatcat ctttttgctg tatgttatat
    20341 gttggtttgt gcttttttat taatgagctt tcaagaagca gagcttcaat ttcactctga
    20401 gtaacaatat agtcaatatc ggtaatatgt gaaacaagaa cacgtgtttt agcatctaag
    20461 tttttctgaa agtacgaagc aacgcgtttc tttaatgatg aagctttacc aatatagata
    20521 atctcttttt tttggttaat gaatagatat acaccaggtt ttgatggcag gttttttatt
    20581 ttctgttcaa gattgttttt catttactgc atagtgttat tgtactgctt ttatttttcc
    20641 aatatagcgc aatacaagtt cattgtcagg gtttaattct ctggctttct caaagtattg
    20701 tagtgcatca ttaaacttct ctttttttgc taaacacact ccagcattaa aatagcttgc
    20761 aaagctatga ggtttatata gtaaggttac agtaaagcat gccagggctt catccagctt
    20821 cccatctttc agtaatacaa cacctttttt aaaaatatct gaaagtgttt cttcttttac
    20881 tgtatgcaac atttggtcag tgtgtttgat cttttctaca aggctattaa atgaggttgc
    20941 tattggtttg agttcgctat caatgctgtc attaagggta acatcaagag atcctgttgc
    21001 aatactttga atattgtgcg atagttcttg aatgagtgac tttgtgagat aggagtgaat
    21061 cattgatatt ataataataa taatgatggt tgcaacaatt actatggcaa taacttgcat
    21121 taatgttttt ataagtgcat catatttttg ctgtaaaatt gtaatattgg gtgtaagcca
    21181 ttgtataatt aaacactggg gtttgctatt gccatgaagt ggaatatagc tttccatata
    21241 tgtaatggta ccttgttttt ctattaccgg attaatggta acatgtgcaa agcctttgcg
    21301 tatcaaaaat tcctgtgatg tatcaacgcg ggttaaattt ttaatctgtg gtttcccttt
    21361 aatgactgtt gttatggtat agtttctgtc atcatcagtt ggctttacaa tatatgctgt
    21421 atacactgag atattgtttt taacaggcat aaatagtgca tgttcagttg ttttattatc
    21481 agtatttact ttatttaaaa aattatataa caccatgctc atggattcaa cgtgatcaat
    21541 ggttgccctg tgtgcataat caagtgaagt tgtatatatt tgtgaaatag tttgagttgt
    21601 tataa
//
LOCUS       DAORYL010000053        21253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_2110_length_21253_cov_12.440891, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000053 DAORYL010000000
VERSION     DAORYL010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 21253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21253
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2110_length_21253_cov_12.440891"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1438)
                     /gene="flgE"
                     /locus_tag="PLV81_10135"
     CDS             complement(68..1438)
                     /gene="flgE"
                     /locus_tag="PLV81_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HOM10494.1"
                     /translation="MMRSLFAGVSGLKNHQTRMDVIGNNISNVNTYGFKYSRVTFQDM
                     LSQTISGAAKPEENRGGINPRQVGLGMNIAAIDKIFTQGSLQTTGVNTDMAISGDGFF
                     IVADGQKHLYTRAGTFALDKDGTLVNPANGLKVQGWMATTNAAGEKVINTSGTVEDII
                     VPIYGKVPAKETNYVKYKCNLDSKLPVVPNDANAIMRTSAGVTTNIDIYDKLGNPHRM
                     TLNFWKSGVNEWTASAAITDTELVTLDVPNGGNQPNQINGSTRVVLKFSPEGRIVSVA
                     DANSPDEINQGKLEVNLSYRVAGDPTLRNIRLDFGSSGLVEGITQFSSPSTTKAVEQD
                     GYAMGYMESFTVDNSGIITAVYSNGTKQMIGQVATAVFTNPQGLIATGDNMFEVSNNS
                     GLPLIGPAGEAGRGKIVAGTLEMSNVDLSEQFTDMIITQRGFQANSRTITTSDQMLQE
                     LINLKR"
     gene            complement(1459..2073)
                     /locus_tag="PLV81_10140"
     CDS             complement(1459..2073)
                     /locus_tag="PLV81_10140"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HOM10495.1"
                     /translation="MPDALKMSGEDVAKAQFQANEVNAKLKAKKGKLPSELDKDSFLK
                     LLVAELRHQDPTQPMNDREFISQMAQFSSLEQMSNMNQSIEKLVLRSQSSDAFSLIGK
                     EVEAVDFASQQIVRGLVSGVVYRQDGVKVIIGSAEVSLNDIHAVYLPGNYRTEKNDAA
                     ANTDVTVKQEIKENVAQEKQNAVEAYSTNAFSKGSSSHIEPVRN"
     gene            complement(2084..>2915)
                     /locus_tag="PLV81_10145"
     CDS             complement(2084..>2915)
                     /locus_tag="PLV81_10145"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOM10496.1"
                     /translation="KETLHDTINQLQKLVDGNSSAKDIRKSNLKQLIAHLTTMQHTGK
                     KDLHSVLINQPAIHYKELSDNKNKKMVRKSGNDVEPLHADDKAYKTVKNSETTLLDAV
                     KQKGRTEDSPKLDKKAGQATDRHDSFIQVNAKQNLSTSATATKPVVHDQLMQLLSKAK
                     VMQEGEKTSLSLKLYPESLGKLSVNLGLEHGILSGRFIVESQEAKELLLQQLESIRWE
                     LQNNGVQVGAFEVNVKEHHQREFTEMPGLSHKQTIENTEYEVASNRYIYHDGLLDVII
                     "
     assembly_gap    2916..3015
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3016..3239)
                     /locus_tag="PLV81_10150"
     CDS             complement(<3016..3239)
                     /locus_tag="PLV81_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10497.1"
                     /translation="MIKPVVLDFEYNVQSTQNIKNNVPELSFSSILKDAMQNTSEATD
                     PECNQTDDSVNHYSNSATVQVKDTKEKDYD"
     gene            4026..5294
                     /locus_tag="PLV81_10155"
     CDS             4026..5294
                     /locus_tag="PLV81_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOM10498.1"
                     /translation="MEAIESLQNTEIQEPPDFSTSNTDEPPSACNTQSNVITILPSRH
                     VVHKPSTSNKTKIFRKLFYPDIIDSQWNDWRWQIANRIRTYEELSAILELTENEKNAL
                     ISHTQLPMSITPYYASLIERNNPFDPIRRCVVPTIHELEHNHGESEDPLSEDDDSPVP
                     GLVHRYPDRVLFLATDFCSTYCRYCTRSRIVGRSNCTLPGKNWDMALRYIAEHTEVRD
                     VLISGGDPLTMNDSMIEYLLNRLRAIPHVEIIRIGTKVPAVLPQRITHLLVSMLRKYH
                     PLWINIHFTHPDEITPETMKACNRLANAGIPLGSQTVLLKGINDNVETLKKLYHLLLQ
                     CRVRPYYLYQCDPIVGSQHFRTSVAKGLEMIEGLRGHTTGLAVPHYVIDAPGGGGKVP
                     LLPTYYQGMEGDSVVLKNYEHKYFYYPETT"
     gene            5306..6325
                     /locus_tag="PLV81_10160"
     CDS             5306..6325
                     /locus_tag="PLV81_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOM10499.1"
                     /translation="MRVGITYDLRDDYRAMGYDEEAIAEFDSIETINAIDTTLQQLGF
                     ITERIGHIKHLVSRLASGQRWDIVFNIAEGIHGIGREAQIPALLDAYTIPYTFSDPLV
                     LALTLNKAMTKQIIRDSNIPTPPFMLVTGIDDIKRCSLAYPLFAKPYAEGTGKGITAS
                     SVIRDAGELELTCTQLLQKYKQPVLVEEYLPGREFTVGIVGSGNNAKTIGTIEIILLD
                     NAEKDVYSFINKEQCEERVEYRLADDITAQKSADVALKAWRALGCLDAGRVDIKCDSS
                     GNPHFLEVNPLAGLHPTHSDLPIICSKAGILYQQLLATIMHNALTRYNMLDNAPQKLS
                     SLAIK"
     gene            6435..7442
                     /locus_tag="PLV81_10165"
     CDS             6435..7442
                     /locus_tag="PLV81_10165"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOM10500.1"
                     /translation="MDIIFIHQYVPDDAPLDEKDVLEQRDELAEIFIQMGYTVGSRAI
                     QSHLDLLSLQELSRSSCIVFNLCESLFGRDDLMFLAPFMLEQYGIPFTGSGSHAIALS
                     CHKLESKKLMMQYGIPTPAYITGNTEYHETLNGTYIIKPYNTHASINITQDSIVQIQS
                     RSDFNVLKEKLMASPNLFAEEYIDGRELNISLLLTDDGVQILPHAEIHFDNYPPDKYK
                     IVDYEAKWIHNSFAYENTNRSFSFTEEDRPMLHEIDRISFKLWDIFKLNGYARIDFRV
                     KDKEVYVLEVNANPCISIYSGFMAAVEEYGLTFAQALDIIIKHPVIPKKHLCEADEAC
                     N"
     gene            7426..7926
                     /locus_tag="PLV81_10170"
     CDS             7426..7926
                     /locus_tag="PLV81_10170"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOM10501.1"
                     /translation="MKHVIKNLIQYRDFPVQTDCDTVVSILDATGFFYPEEIDIALEL
                     LNEKINYPDSSSYEFIFAEYQTTVVGYICYGRIPLTKASWDVYWIAVHPDFQGNGIGY
                     TLLSKAEETIKLHNGKHFYIETSGRKLYEPTHTFYLKAGYTVSAVFKDFYADGDDKLV
                     FYKKLV"
     gene            8092..8325
                     /locus_tag="PLV81_10175"
     CDS             8092..8325
                     /locus_tag="PLV81_10175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HOM10502.1"
                     /translation="MECSCGGILTEGRSSYSVSGQHFYLVIEDIPAYKCNRCGKILFE
                     DDVVDKIKALLKRVERDTKEIVTGTPSVHTYDY"
     gene            8355..9791
                     /gene="gltX"
                     /locus_tag="PLV81_10180"
     CDS             8355..9791
                     /gene="gltX"
                     /locus_tag="PLV81_10180"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOM10503.1"
                     /translation="MRVRFAPSPTGFLHVGNARTAVLNFLFKKKYNATYILRIEDTDM
                     ERSTRESEESILNDLKWLGIQWDEGPDCGGTFGPYRQSERFDIYREYTEKLLQGGHAY
                     HCYCTQEELDAQRQKAAQDNKPFVYPGTCRDLTLEQKLAFEKQGRKPTVRFKVKDGIT
                     LSIDDQIKGNVVFNSENIGGDFIIVRSDGVPVYNYIVIIDDTLMNVTHVIRGEDHLPN
                     TPKQMLIAMALGLPVPVYAHMPLLLGPDRTKLSKRHGITSVAVYREQGYLPQALFNYL
                     ALLGWTLESGEEIVPVERLVAEFELSKIGKSSAIFDFQKLKWMNSVYLRNLPFDKAFE
                     LFYPYIEKAYPTVSNSEWLTQVVDTVRGNCEIASDIVKEISIFFGDTAEFEEDAKNML
                     ATPEGKTIIQTAYELIHSTINESNYSTETLNTIKQKTGLKGKMLFMPFRAMVTGRLHG
                     PELDKILPLLGYENFTKRIEYCHQRFVK"
     gene            10054..10743
                     /locus_tag="PLV81_10185"
     CDS             10054..10743
                     /locus_tag="PLV81_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10504.1"
                     /translation="MKKLGSTIIVFTLLTVCACSAHAPGTIPVGTWQYDLLVNGVEMG
                     KATIQNSIEDNQYVYRFDLTIGNGTTRSQDTIVETLECKPIKLEKHTYIKQTTTEQKM
                     DIIAQCNGRTIILIENNMKSIITLDQDYVLDGNYFLYMLIQNKFKKGLQITHNVYDPS
                     IEREAAIPVTIKLIGTETISVNSILYKAWKIEQSIGKIKNIIVYLDEHGVVLKSSIKM
                     LNMQLDIIRKD"
     gene            10746..11525
                     /locus_tag="PLV81_10190"
     CDS             10746..11525
                     /locus_tag="PLV81_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOM10505.1"
                     /translation="MKKIYAIVISAIVGGYLLYLYSQPLPASLSTVPIANITSKLWEE
                     NISLFMVSSDEAFSITQTPFQKILSSRNKNTSYAYIIASGKNKEYHQNPVEKDLKDTR
                     LLQIHNPVIVQHAKTIASSSNPIEEAEELVYNTIETKTEGIPIIPAPAIFELKKGDCT
                     EHAIATIALLRALHIPARAVVGMIAAKEYAGKKNVFVFHMWAEAFYNNAWLLVDATRP
                     GKKKTNIYIALTYHSLETEMPLEFLEAMANIQNLKVTYLSE"
     gene            complement(11506..13014)
                     /gene="murJ"
                     /locus_tag="PLV81_10195"
     CDS             complement(11506..13014)
                     /gene="murJ"
                     /locus_tag="PLV81_10195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOM10506.1"
                     /translation="MNSVRSTTHIAIATALSRLLGFVRDMLIAWMFGASWQIDAFFVA
                     FRIPNLVRRLVAEGTLSLPLVSVIMSYRHKEERKIIANDLFSLYIMSGFLISVTGIVA
                     APAIVKILGFGFTGSVYSLSVVLLRIMFVYCLCASIVAYAMGVLNTEGVFFMPALSPL
                     LLNVGIILFCFIAYLYSNVIWLGVGVVAGGILQCVVVFIPLYKTGVHLTLGHIQSGHT
                     KVLRMIVASVMGVAVYHINVLVNTMMASTLKQGSVSYLYYADRFIEIVSGVFIISMSN
                     AFHPEVSRLWAGRDVARLQATMTSMLQASLFIALPASAGLIATGKVILATFFEHGNFY
                     SFETAMTYKALACAAFGIPAFAVVRILMPLFISMRSSVFINVISTVAIILNAVLGFVL
                     MRTHLLHGGLALANSISLYCVAIALVIKLLHSIKVKVDKRFTVTLLQYFIASGIMGIS
                     LYILQSFLPYEKGLTYTVVLVTLGVVLYAILCKLINPGMTLRILLDAIQKDM"
     gene            13185..14723
                     /locus_tag="PLV81_10200"
     CDS             13185..14723
                     /locus_tag="PLV81_10200"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM10507.1"
                     /translation="MKHAFVYTVCSCILLSVIIAFATPSRAGSYYEAGCRYYVHKNWE
                     KAKENFLKDIETTDRGDSYYFVGEIEKNNGNLTTALEYFEKAVERKMTPKYRNLAYWN
                     LIVIYESRSDFPKTIKTCKYFYEVLGEASAKSKAENLINKLMWTENEDAKKEYEEGLN
                     SKSKDYSLALQHFRQAIMLDSAFLSPVFEIGMYYYTQNQYRDALSYFNQILYRIPFYG
                     ELQLLVGEIYFQEKRYSEAATAFEAALEYAFIDKDMKYSSQLKVATAYYNIGQYTKAE
                     TFLQQAISTKKGNLSPYLLLSAIQMQKNDYDGALQTLQAAYKIDSNNPEVLYQLGLVF
                     YNKNDNKAYEYFEKVYMQYRQKHKEIPAKYIKAFIITAQYYYTTKQYSKVVELTTIVP
                     ENLRDESLSLNYARSLYMLAQYNEAIVHFNKFRLSDNDLFILCKCYAFINDTSKVKEI
                     LSSNYYNTNFINKVKEDKLLKSIYTEVQKQRETPATVQPQPQEEQKDLPATHETTTEP
                     IHPQ"
     gene            14689..15537
                     /locus_tag="PLV81_10205"
     CDS             14689..15537
                     /locus_tag="PLV81_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10508.1"
                     /translation="MKLLQNPYIRNNSITIIQALIIATSVIALFHVFHLLHIIIIAFI
                     VSLGLQVWCQLNPCYADYITSAEAPLASLALLITMLISHSMSLSIVVTFIVLFIYHYF
                     FSSYELYHDIYFTALLFSIILIFVSLYLNNMGIFNDYTIKIFTGVQSNTSLSIAIISI
                     LMSILYTYLSKLKYEFRIISLGHNYINLLGIDENIIHGISSIAKALISTMIFILIGWM
                     GSLLTTIPLLRKSSHPLYIMIFTAMLSLLLYAIHFVHPFYIIIPVVIIDFVYVFFLGK
                     RLPCSG"
     gene            15525..16298
                     /locus_tag="PLV81_10210"
     CDS             15525..16298
                     /locus_tag="PLV81_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOM10509.1"
                     /translation="MLRLNNLSLIITDNHILKNITVEFFEGEIIGIIGKPGSGKSVLL
                     QTIAQYYTYYNGEILLNAKPIHLIKKRDLAQKISYQPAGYFYNHYITLYDYLVSSRIP
                     YKTYLSPFQSFDYEIVDKYMYHFELQQYKSKPLMLLSSDIQARALLAFHFIRDAQIVM
                     LDNPTQNLSLPSYNQLHDAIIRYCSTGKNCCIIASHDINFLAQTVDKLYIMENGSIVD
                     SCVPADLTEDLIKKIFGVDVFITRNIYNGKPNVHLYFEY"
     gene            complement(16347..16694)
                     /locus_tag="PLV81_10215"
     CDS             complement(16347..16694)
                     /locus_tag="PLV81_10215"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOM10510.1"
                     /translation="MAAEINYSIEEREGAKIVNLAGVLCSATEREFISLIESLVATNN
                     VIINVSGVSMVTSSGLKALTDLSHFARQKKRRVMILGASDNLQSLINATESYDLLLIN
                     SVEEGIRKLDYYI"
     gene            complement(16694..17788)
                     /gene="aroB"
                     /locus_tag="PLV81_10220"
     CDS             complement(16694..17788)
                     /gene="aroB"
                     /locus_tag="PLV81_10220"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOM10511.1"
                     /translation="MDPITNSDTLVLQLDSKKSTIIIGKGILASLSSMIPYKKWDVIV
                     AIVSDTVYSLHKEVIDQSLSLIDAIPIHYNDSEQNKSYAYAEQYFNLLLENQCTRNSL
                     VIGIGGGVTTDFAGYVASAYMRGIDVVYVPTTLLSMVDASIGGKVAVNIKAGKNIVGH
                     FHQPDYIIIDIDFLQTLPQSEWRCGMSEVVKHALIGDSSSLTLLLNKEIQYGHIDNSL
                     YSCINSSVRFKASVVEKDEYEKNIRAILNFGHTVGHAIESLLEYTVSHGDAVAAGMLI
                     ESYISMKEGMPKADFDCIRDIISAYSLLPNISFDPNKIVNHMMYDKKNIKGKTRFVLL
                     ESIGKPRIGCEVNDGLVKEALNFYLTMVGK"
     gene            complement(17776..18117)
                     /locus_tag="PLV81_10225"
     CDS             complement(17776..18117)
                     /locus_tag="PLV81_10225"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOM10512.1"
                     /translation="MPGGGVEYQESLDEALKREFFEELGLAVEVGDIALIADSIEPHG
                     KRHIINIVFWCYLLNKSIRLGDEKILYDFSFKPVSELADIVMYPPLNEYIIQLLQNKN
                     ERIYKGSLWIP"
     gene            complement(18098..18232)
                     /locus_tag="PLV81_10230"
     CDS             complement(18098..18232)
                     /locus_tag="PLV81_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10513.1"
                     /translation="MRFELSHKKIRIRVAGLVVEENKILMIAHKKGGGNLLVVAWRWC
                     "
     gene            complement(18229..18972)
                     /gene="rnc"
                     /locus_tag="PLV81_10235"
     CDS             complement(18229..18972)
                     /gene="rnc"
                     /locus_tag="PLV81_10235"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOM10514.1"
                     /translation="MDDENKKGLDKKRQNRKLQQLEKRLHFTFSNKSLLKRALTHRSY
                     VNESSIPVKDNERLEYLGDSVLGLVVNEYLFKSFENYPEGDLAKIKSAVVSEDTLAKV
                     ANQMNLGDYIIMGRGEELTGGRYRQSILADALEAIIGAVYLDAGLTKSKKLVLRLLKK
                     DIQRIDKLSYLRDPKTTLQELVQKKYKSRPLYTVIHESGPDHSKMYTIKLTINDRDIT
                     VGTGSSKRKAESDAAKKALELIYSNRITI"
     gene            complement(19000..19236)
                     /locus_tag="PLV81_10240"
     CDS             complement(19000..19236)
                     /locus_tag="PLV81_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOM10515.1"
                     /translation="MTVDFEKIKKIIVDQLGVDESEVTLQASFVDDLGADSLDTVELV
                     MALEEEFGIEIPDEDAEKLLTVEDVVKYIEAKLQ"
     gene            complement(19383..19577)
                     /gene="rpmF"
                     /locus_tag="PLV81_10245"
     CDS             complement(19383..19577)
                     /gene="rpmF"
                     /locus_tag="PLV81_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOM10516.1"
                     /translation="MAVPKRRKSKAKSRMRRSHDAIGAPNLRPCPTCGAYVLPHRVCP
                     ECGHYKGKLIVEPVVKVNKG"
     gene            complement(19670..20374)
                     /locus_tag="PLV81_10250"
     CDS             complement(19670..20374)
                     /locus_tag="PLV81_10250"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM10517.1"
                     /translation="MIDREVTVHRNIVEQMLMDVRDYIRAHRSVAIKVAIGVSVVLVL
                     GIAAIVYADYKIKKDTADFEAILDEYRTTYTTDIQEHNEKFLRTVKRLQTIADSSYFG
                     YVHRNGYYIIASLYFNEKDYANAARYYEKAAQYASPLFASLALQQAGMSSEYLNNIDE
                     ALRYYLLCESKYKDAFNIDQILYSIGRMYALKGEYYKAQEYFNRVTTEFPQSFFAKKA
                     AQHSIFLGAIENEKKN"
     gene            complement(20378..>21253)
                     /locus_tag="PLV81_10255"
     CDS             complement(20378..>21253)
                     /locus_tag="PLV81_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOM10518.1"
                     /translation="WLSVPEKYQPGNTIEGKIVTITNFGMFIEIEPGVEGYCSASDIS
                     WAKRTVNPKELYKKGDRIQSVVLSVDDENRKLLLGIKQLQPNPWETIEERFPVKSIHT
                     RPIKKIVNFGMFVELEPDIDGLIHISDISWDENFKDIQSQYKEGQEIQFIILEINKEE
                     QKIACGIKQLTKSPWEVIAEKYPPRTIVSGIVTGIVPFGMFVKIDGDTVEGLVHISEV
                     SRKKIESVDTIYKVGDKVNAVVLGVDVDKKRLSLSIKHYEIMSEKEEIKKILNTSTSG
                     TVTLGELLKNKLDKE"
BASE COUNT         6650 a         4320 c         3601 g         6582 t
ORIGIN      
        1 attaattcct gaagcatctg tttacaacaa tggggttttg aaaaaacccc attccctact
       61 atttatatta ccgcttcaga ttaattaatt cctgaagcat ctggtcagat gttgtgatag
      121 ttcgggaatt tgcctggaat cctctctggg taattatcat atcagtgaac tgctcagaaa
      181 ggtcaacgtt tgacatttca agtgtaccgg ccacaatttt ccctctgcct gcctcacctg
      241 ctggtcctat taacggtagc cctgagttat tggatacctc aaacatatta tcgcccgttg
      301 ctattaaccc ctgaggattg gtaaacactg cagtggcaac ctgccctatc atctgcttgg
      361 tgccattaga atacacagca gtgatgatgc ccgagttatc cacagtaaat gattccatat
      421 agcccatagc atatccatcc tgttcaaccg ccttggtggt tgatggggat gagaactgag
      481 ttataccttc aacaagccca gaactgccaa aatcaagcct gatattacgt agtgtggggt
      541 cacctgctac acgatagctc aaattaactt caagtttgcc ctgattaatc tcatcgggac
      601 tgtttgcgtc agcaacagat acaatgcggc cttcgggtga aaattttagc actaccctgg
      661 ttgacccgtt tatttgattt ggctgattgc caccattggg cacatcgagt gttaccagtt
      721 cggtatccgt gatagcagca gaagccgtcc actcattcac cccactcttc cagaaattga
      781 gcgtcatacg gtgtggatta cccaacttat catagatatc aatattggtt gtcactccag
      841 cagatgttct cattattgca ttggcatcat tggggactac cggcagcttt gagtccagat
      901 tacatttata ttttacataa ttggtttctt ttgcaggcac tttcccataa ataggaacaa
      961 tgatatcctc aaccgtaccg gatgtattga taaccttttc cccggcagca tttgttgtag
     1021 ccatccatcc ctgcacttta agtccgtttg caggatttac cagcgttcca tccttatcaa
     1081 gtgcaaatgt gccagcacgt gtatacaggt gcttttgtcc atcagcaaca ataaagaatc
     1141 catcaccgga tattgccatg tctgtattga caccagttgt ttgcaagcta ccctgagtaa
     1201 atatcttgtc aatagcagca atattcatac ccagaccaac ctgccgggga ttaatcccgc
     1261 cacggttttc ttcaggctta gcagcacctg aaatagtctg cgacagcata tcctgaaagg
     1321 taaccctgct gtacttgaag ccataggtat taacgttgga aatattgttg ccaatgacat
     1381 ccatccgtgt ctgatggttt ttaagtcctg aaacacctgc aaaaagtgat cgcatcatat
     1441 cgcaaaactc ctgttaatct aatttctaac tggttctata tgtgatgaac tgcctttact
     1501 gaaggcgttc gttgaatatg cttccacggc gttttgtttt tcctgtgcca cgttttcctt
     1561 tatttcctgt tttactgtaa catcagtgtt tgcggcagca tcattttttt ctgtgcgata
     1621 gttaccgggt aaatagaccg catggatatc attgagcgaa acctcagcag aaccaataat
     1681 caccttgact ccatcctgcc tgtacacaac accgcttacc aacccgcgta caatctgctg
     1741 gcttgcaaaa tcaaccgctt caacctcttt gccaataagt gaaaatgcat cactggactg
     1801 ggaacgtagc accagctttt caattgactg attcatattg ctcatctgtt caagcgaaga
     1861 gaattgtgcc atctgactta taaattctcg gtcattcatg ggctgggtgg ggtcctgatg
     1921 gcgcagttca gccactaaaa gcttcaagaa agagtcctta tcaagctctg atggtaattt
     1981 acccttttta gctttcaatt tggcattaac ttcatttgcc tgaaattgtg cttttgctac
     2041 atcttcgcct gacattttta atgcatctgg cattgtatgc tccttatatt ataacatcca
     2101 gtaatccgtc atggtatata tatcggttgc ttgccacttc atattcagta ttttcaatag
     2161 tttgtttgtg tgacaatcct ggcatttcag taaattcacg ctgatgatgc tctttaacat
     2221 tgacttcaaa cgcacctacc tgcacaccat tgttttgtaa ctcccagcga attgattcca
     2281 gttgttgaag cagcagctct tttgcctcct ggctttcaac aataaaacga ccactcaaaa
     2341 taccatgttc cagtcccagg tttacagaaa gtttacctaa actttctgga tataatttca
     2401 atgaaaggct tgttttttca ccttcctgca ttactttagc tttgcttaaa agctgcatca
     2461 gctggtcatg caccacaggt tttgtagcag tagcgctggt actcaaattt tgctttgcat
     2521 ttacctgaat aaatgagtca tggcggtcag ttgcctgccc tgcttttttg tccagctttg
     2581 ggctatcttc tgttctccct ttctgtttca ctgcatcaag aagtgtcgtt tcactgtttt
     2641 ttactgtttt ataagctttg tcatcagcat gaaggggctc tacatcatta ccactttttc
     2701 gtaccatttt tttatttttg ttgtccgata gctccttgta atgaatcgca ggctgattta
     2761 ttagcacact gtgcaaatcc ttcttgcctg tgtgctgcat tgtagtaagg tgagctatca
     2821 gttgttttaa gttactcttc cttatatcct ttgcagaact gttaccatca acaagctttt
     2881 gcaattgatt aatagtatca tgcagtgttt ctttcnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnctatc ataatctttt tctttagtat ctttcacctg cactgttgca
     3061 ctatttgaat aatggttcac tgaatcatct gtctggttac attcaggatc cgttgcttca
     3121 ctggtatttt gcattgcatc tttcaatata gaactgaacg atagctccgg tacattattt
     3181 ttgatgttct gcgtactctg tacgttatac tcaaaatcca gcacaacagg ttttatcata
     3241 tttataccct gtaataagat gaagtcttac cattaatatc gaatgacccg ttttttttaa
     3301 tgaattaatt aagcaattat tacaaaaaaa tatttttatg aacgaaagaa caacactttt
     3361 gcattatgtc atattagctg ttatttttaa ccttaatttc aagaggactt aaaatcctaa
     3421 tataacacgc tgtatcaaga attcttctct cttcgttttt aaaaccgaca ttgcaaaaaa
     3481 aatgttattc taaatgacat caagatgacc gcttgtggct atcaaataca caaaattagc
     3541 caaaataaaa atattaaaat tttaatataa aaatataaaa aattttagtt gcaatactat
     3601 acgtgtgata tatatggtac tgtaacttct gcgctattac ctgcaatgtt taacgcaggt
     3661 ttatagcggc attatttgga atagaacttt ccataattgc cgatcctcca ttttttatgg
     3721 atcccagact cacgtcccca aaatgggaga cctcttggac actcatccct gtcaaaagca
     3781 gggatgcttt ttttatattt ttttattttt tttctttaca tgctgtatat ttgtttttaa
     3841 ttgcatttta ttttttagta tatatgccga ttcaaatact ataaatttta tagtagaaaa
     3901 atgtaatttt tttgcgacat aatacggtat ttttcttgac aaaatacttt ttttatattt
     3961 gtaagaaata gattatacat tgtgatttaa aaatcttctt ttgataaagt gaggtacgca
     4021 aatagatgga agcaattgaa tcactgcaaa atacagaaat acaggagcct ccggattttt
     4081 ccacctcaaa cactgatgag cctccctcgg cgtgtaatac acaatcaaat gtgataacaa
     4141 tcctgccttc acggcatgtt gttcataaac cttccacaag caataaaaca aagattttca
     4201 gaaagctttt ttatcctgat atcattgatt cacaatggaa tgactggcgg tggcagattg
     4261 ccaacagaat acgtacatat gaagaactat ctgcaattct tgaactcact gaaaatgaaa
     4321 agaatgcgtt aatcagccac acgcagttac ccatgtcaat aacaccctat tatgccagtc
     4381 ttattgagcg caacaatcct tttgacccaa ttcgccgctg tgttgttcca acaattcatg
     4441 aactggaaca taatcacggc gaatctgagg acccgctttc agaagatgac gacagcccgg
     4501 tcccgggatt agtgcatcgc tatcccgata gagtgttatt tttagcaacc gatttctgtt
     4561 caacatattg ccggtactgc acccgttcac gcattgttgg tcgctcaaac tgtacactac
     4621 caggtaaaaa ctgggacatg gcattacgct atattgctga acataccgaa gtccgtgatg
     4681 tacttatttc tgggggcgac cctttgacaa tgaatgattc aatgatagag taccttctta
     4741 accgtctcag ggccattcca cacgttgaaa ttatccgcat tggtactaaa gttccagcgg
     4801 tgttgcctca gcgtatcacc catttactgg taagcatgtt acgaaaatat catccattat
     4861 ggataaacat acactttacc catcctgatg aaataacccc tgaaaccatg aaggcttgta
     4921 acaggcttgc aaatgcaggt attcctttag gaagccagac agtgctgcta aaggggatta
     4981 atgataatgt tgaaactctg aaaaaactgt accatctgct tttacaatgc agggtacgcc
     5041 cctattacct gtatcagtgt gaccccatag taggttcaca gcatttccgc acttcagttg
     5101 caaaaggtct tgaaatgatt gaagggctca ggggacatac aaccgggctt gcagttcctc
     5161 actatgttat agatgcaccg ggaggtggag gtaaagtacc actgttgccc acatactatc
     5221 agggtatgga aggggattct gtggtcctta aaaattacga acataaatat ttctactatc
     5281 ccgaaacaac atgaccagca gagccatgcg tgttggcatt acctatgact tacgagacga
     5341 ttaccgggca atgggctatg atgaggaagc tattgccgaa tttgacagta tagaaacaat
     5401 taatgccata gataccaccc tgcagcaatt aggttttatc accgaaagaa ttggccatat
     5461 taaacatctt gtttcacgcc ttgccagcgg tcagcgctgg gatatagtat tcaatatagc
     5521 tgaaggtata cacggtattg ggcgtgaagc gcagattcca gcactacttg atgcctacac
     5581 cattccctac accttttctg acccacttgt gctggcactg acattaaata aagccatgac
     5641 aaaacagata atacgcgata gcaatatacc cactccccca tttatgcttg ttaccggtat
     5701 tgatgacata aaacgctgtt ccctggcata tcctctattt gcaaaaccat atgccgaggg
     5761 aaccggtaaa ggtattactg cttcttcggt cattcgtgat gcaggtgagc ttgaattaac
     5821 ctgtacacaa ttgcttcaaa aatataagca accagtactg gtggaggagt acctcccggg
     5881 aagagaattt actgtaggga tagttggtag cggcaataac gcaaaaacta ttggaactat
     5941 tgaaatcata ttactggata atgccgaaaa agatgtgtat tcattcatca ataaggaaca
     6001 atgtgaagaa cgggtagaat acaggcttgc agatgatatt acagcacaga agtcagcgga
     6061 tgttgcactt aaggcatgga gagcacttgg atgtcttgat gcaggccgtg tggatattaa
     6121 atgcgatagc tccggtaatc cacactttct tgaagtaaac cctcttgccg ggcttcatcc
     6181 cacacattcc gacctgccaa taatttgctc taaagctggc atattgtatc agcagttatt
     6241 agcaaccatc atgcataatg cgttaacacg ttataacatg cttgataatg caccgcaaaa
     6301 attatcttca ttggctataa aatagtactt gcacaaatac tataacataa aaaatttgta
     6361 ataagtggtt tatacacacc aaccctgaat caccggtgtg caattgtaaa ttccatttca
     6421 ataacgttgg aatcatggat ataatcttta ttcaccagta tgtacccgat gacgctccgc
     6481 tggatgaaaa ggatgtgctg gagcagcgag atgaacttgc tgaaattttt attcagatgg
     6541 ggtacactgt aggttcacgg gcaatacaat cacatctgga cctgctatca ttgcaggaac
     6601 tttcgcgcag cagttgtatt gtatttaatc tgtgcgaaag cctgtttggg cgtgatgacc
     6661 ttatgttcct tgcacctttt atgcttgagc aatacggcat tccatttacc ggttcagggt
     6721 cacatgccat tgcactaagc tgccacaagc ttgaaagcaa aaaactcatg atgcagtatg
     6781 gcattcccac cccggcatac attacaggca ataccgagta tcatgagacc ctgaatggca
     6841 cctatattat caaaccatac aacactcatg catccatcaa tattacccag gattcaattg
     6901 tacagataca atcccggagt gatttcaatg tactaaaaga aaagcttatg gcttcaccca
     6961 atctttttgc agaagaatat attgacggaa gggaattgaa tatttcactt cttctcactg
     7021 atgatggagt acagatactc ccccatgccg aaatacattt tgataactat ccgcccgata
     7081 aatataaaat tgttgattat gaagcaaaat ggattcataa ctcatttgcg tatgaaaaca
     7141 ccaaccgaag cttttccttt accgaagaag acaggccaat gttacatgaa attgacagaa
     7201 tatctttcaa attatgggat attttcaaat taaatggcta tgcacgtatt gatttcaggg
     7261 taaaagataa agaagtctat gtgcttgagg ttaatgctaa cccctgcata tcaatttaca
     7321 gcggctttat ggctgcagtg gaggaatatg gattaacctt tgcacaggca cttgacatta
     7381 ttattaaaca tcctgttatt ccaaaaaaac atctatgtga agcggatgaa gcatgtaatt
     7441 aaaaatctta tccagtacag agatttccct gtccaaaccg attgcgatac agtagtatca
     7501 attcttgatg ctaccggttt tttttatcca gaagagatag atattgcctt agaactatta
     7561 aacgaaaaaa taaattatcc tgatagctca agttacgaat ttatttttgc tgaataccaa
     7621 acaactgtag ttggatatat atgctatgga aggatcccat tgacaaaagc ctcatgggat
     7681 gtgtactgga ttgcagtaca tcctgatttc cagggcaacg gtattggata caccttgctc
     7741 tcaaaagcag aagaaaccat taaattacat aacggcaaac atttttatat agaaacatcc
     7801 ggcagaaaac tgtatgaacc cacccacaca ttctatctga aagcaggata cactgttagc
     7861 gcagtattta aggattttta tgctgacggt gatgataaac tggtatttta taaaaaatta
     7921 gtataaaatt tacagcattg tttggatttt gaaggcagtg aatctttcta tgtattctca
     7981 tattgtacag taactatcgc cccaattaat attttctttg cttttttata atttccatta
     8041 gaatcttgac aaatagagtg ttacattgat gtatattact gtgaggtacc tatggaatgc
     8101 tcatgcggtg gtatattaac ggaaggacga agctcgtata gtgttagtgg gcagcatttt
     8161 tatttagtca tagaagatat cccggcctat aaatgcaacc ggtgcggtaa aatattgttt
     8221 gaagatgatg tcgttgataa aataaaagca ttactgaaac gagttgaacg ggacacaaaa
     8281 gaaattgtta ccggcacacc ttcagtacat acatacgact attaattttt ttacacatac
     8341 aggagaacat aaccatgcgc gtacgatttg cacccagccc aacaggcttt ttacatgtag
     8401 gtaatgctcg aactgcagta ttaaactttc tgttcaagaa aaaatataat gccacatata
     8461 ttctcagaat tgaagatact gatatggaac gcagtaccag ggaatctgaa gagtccattc
     8521 tcaatgactt aaagtggctt ggcatacagt gggatgaagg ccctgattgt ggtggaacgt
     8581 ttggccctta cagacaatct gaacgttttg acatttaccg tgaatatact gagaagctac
     8641 tgcaaggcgg ccatgcatat cattgctact gcacccagga agaacttgat gcccagcgcc
     8701 agaaggcagc acaggataat aaaccttttg tatatcctgg aacatgtaga gaccttaccc
     8761 ttgaacaaaa attggctttt gaaaagcagg gcaggaaacc cacagtgcgg ttcaaggtta
     8821 aggatgggat tactctttca atagatgacc agattaaagg caacgttgtc ttcaacagtg
     8881 aaaatattgg tggtgatttc atcatagtcc gttctgatgg tgttcctgta tacaattaca
     8941 ttgtcatcat tgatgatacc ttaatgaatg tgacccatgt tatccgcggt gaagaccatt
     9001 tgcccaatac accaaagcag atgctcattg ccatggcgtt agggctcccg gtacctgttt
     9061 atgcgcacat gccacttttg cttggacccg accgcacaaa actatcaaag cgccatggga
     9121 ttacatctgt tgcagtgtac cgggaacagg ggtatttacc tcaggcatta ttcaattatt
     9181 tagccctact gggttggaca ttggaatcag gtgaggagat agttcctgtt gaaaggcttg
     9241 ttgccgaatt tgagttatca aagattggaa agagttccgc tatttttgat tttcagaaac
     9301 tcaaatggat gaattcagta tatttgcgta atcttccttt tgacaaagca tttgagctgt
     9361 tttatcccta tattgaaaaa gcatatccta ctgtaagtaa cagcgaatgg cttactcaag
     9421 tagttgatac ggtacggggc aattgtgaga tagcttcaga tattgttaag gagatttcta
     9481 tcttctttgg cgacacagct gaatttgaag aagatgccaa aaacatgtta gcaacaccgg
     9541 aaggtaagac aatcattcag actgcatatg aacttattca ctcaactatc aatgagagca
     9601 attacagtac tgaaacactc aacactataa agcaaaaaac agggctcaag gggaagatgc
     9661 tttttatgcc gttcagagca atggtaaccg gcagattaca tggccctgag ctagataaaa
     9721 tactaccact gttagggtat gaaaatttca caaagcgtat tgaatattgt catcaacgct
     9781 ttgttaaata aattaaacag agtaggttac acaaagcact caaagtatga attttttatt
     9841 tcaaaggcca tgttgaatca taataaatgt attgacaata tctgtgctat aaacacccac
     9901 aatgatagaa atgatattga gaatgactat gcgtggcaaa aagatttctc accccgaaaa
     9961 gtcggggttc gaaatgacaa gttaagattt ctcatcctga tagatagggc tgatataatt
    10021 aaatcgtatt ttccagggga atatatgcaa ttcatgaaaa agttaggatc tacaataatt
    10081 gtttttacgc ttctaaccgt ttgtgcgtgt tctgcacatg cacccggtac aatccctgta
    10141 ggcacatggc agtacgatct tcttgtcaac ggcgttgaaa tggggaaggc cactatccag
    10201 aacagcattg aagacaacca gtatgtatat cgctttgatt tgacaatagg aaatggtacc
    10261 acacggtcac aggatactat agttgaaaca ttagagtgca aaccaataaa acttgaaaaa
    10321 catacctata taaaacaaac aaccactgaa caaaaaatgg atattattgc acagtgtaat
    10381 ggccgcacca ttatcttaat agaaaataat atgaagagta tcataacatt ggaccaggat
    10441 tatgttcttg atggtaatta ttttctgtat atgttaatac agaataaatt caaaaaaggt
    10501 ttacagatca cacataacgt ctatgaccca tcaatagaac gagaagcagc aattcctgta
    10561 accataaaac ttataggaac tgaaacaatt tcagttaaca gtattctgta taaagcctgg
    10621 aaaattgagc agagcattgg taaaattaaa aatattattg tatatcttga tgaacatggc
    10681 gttgtgttaa aaagttctat aaaaatgtta aatatgcagc ttgatatcat ccgtaaggac
    10741 tgatcatgaa aaaaatatat gctattgtta tatctgccat tgttggtgga tacttactat
    10801 acctgtattc gcaaccattg cctgccagtc tttcaacagt tcccattgcc aatataacca
    10861 gtaaattatg ggaagagaat atatcccttt tcatggtatc atccgatgaa gcattttcca
    10921 taacccagac accatttcaa aaaattcttt cgtcacggaa caaaaatact tcgtatgcat
    10981 acatcattgc atcaggaaaa aataaagagt atcatcaaaa tcctgtagaa aaagatttaa
    11041 aagacacgcg attattgcaa atacataacc cagtaattgt acagcatgca aaaacaattg
    11101 cctcaagctc caatcccatt gaagaagctg aagagttagt ttataacaca atagaaacca
    11161 aaactgaagg gattcccatc atacctgcac ctgcaatttt tgaattaaaa aaaggtgact
    11221 gtaccgagca tgcaatagca acaattgcac tgctgagggc actgcacatt ccagcacgag
    11281 cagtggttgg catgatagca gcaaaagaat atgcaggcaa aaaaaatgta tttgtgtttc
    11341 acatgtgggc tgaagccttt tataacaacg cgtggctgct tgttgatgcc actcgtccgg
    11401 gaaaaaagaa aacaaacatc tacattgcat tgacatatca ttcacttgaa acggaaatgc
    11461 ctctagaatt tttagaagcc atggccaaca ttcagaatct aaaagttaca tatctttctg
    11521 aatagcatct aacaatatac gtaatgtcat accagggtta ataagtttac atagtatggc
    11581 atatagcacc acacccagtg taacaagcac cactgtatat gtaagacctt tctcatacgg
    11641 taaaaacgat tgcaaaatat acagtgaaat tcccataata cctgaagcaa tgaaatactg
    11701 cagcagagta acagtaaacc ttttatccac tttcactttt attgaatgca aaagttttat
    11761 gacaagagct atcgccacac aatataatga aatgctgtta gcaagtgcca gcccaccatg
    11821 caaaaggtgc gtacgcataa gcacaaagcc aagcactgca ttcaggataa ttgcaactgt
    11881 tgaaattaca ttaataaata cggacgacct catactgata aagagcggca tgagaatccg
    11941 caccacagca aatgcaggaa taccaaacgc tgcacatgca agtgccttat aggtcatggc
    12001 tgtttcaaaa ctatagaaat ttccatgctc aaagaatgta gccagtataa ccttccccgt
    12061 tgcaataagc ccagcactgg caggaagtgc aataaatagt gatgcttgca gcattgatgt
    12121 cattgttgcc tgcagacgcg caacatccct gccagcccac aaccgtgaaa cctcaggatg
    12181 aaatgcattg ctcattgaaa tgataaaaac tccacttaca atctcaatga atctatcagc
    12241 ataatacagg tagctaacac taccctgctt gagcgttgat gccatcatgg tgttgactag
    12301 cacattgata tgatacacag caacacccat cactgaggct acaatcatac gcagcacttt
    12361 agtgtgtcct gactgtatat gacccaatgt caggtgcaca cctgttttat acaatggaat
    12421 gaaaacaacc acgcattgca aaatgccacc ggctacaaca cccaccccaa gccatataac
    12481 atttgaatac agatatgcaa taaagcaaaa aagaataatg cccacgttaa gcaacagtgg
    12541 cgataatgca ggcataaaaa aaactccttc agtgtttaac acacccatgg catatgcaac
    12601 aatacttgca cacaggcaat agacaaacat gatacggagc aatacgacag aaagactata
    12661 tacgcttcca gtaaatccaa aaccaagaat ttttacaatg gcaggtgctg caacaatacc
    12721 tgtaacagaa atgagaaacc ccgacatgat gtataatgaa aataaatcat tagctattat
    12781 ttttctttct tctttgtggc gatagctcat aataacagat acaagtggca atgataatgt
    12841 cccttcagca accagccttc gcaccagatt aggtattctg aatgcaacaa agaatgcatc
    12901 aatctgccag gatgcaccaa acatccaggc aataagcata tcgcgtacaa agcccagtag
    12961 cctgctcaat gccgttgcta ttgctatatg tgttgttgac ctgacagagt tcatataaaa
    13021 ttaatgtatt gacatatttt ataactatca gcagtaatta ttagcataca tcattaagaa
    13081 atgaatagaa gtattattca ttgtagccag agttgtacat gtaaatactg tacataacat
    13141 tttacaaaat tgccataaca ctatctattt caaggatatt tgacatgaaa catgcttttg
    13201 tatatactgt atgttcctgc attttgttat ccgtaattat tgcgtttgct acaccttcac
    13261 gtgctggtag ttactatgaa gctggttgtc gttattatgt ccataaaaat tgggaaaaag
    13321 caaaagaaaa ttttttaaag gatattgaaa caactgaccg aggcgattcc tattactttg
    13381 ttggcgaaat tgaaaaaaac aatgggaatc ttacaactgc tctggaatat tttgaaaagg
    13441 cagttgaacg caagatgaca ccaaaatatc gtaatttagc atactggaac cttattgtca
    13501 tctacgaatc cagatccgat tttcccaaaa ccattaaaac ctgcaaatat ttttatgagg
    13561 tgctgggaga agcatcagca aaaagtaaag cggaaaatct gattaataaa ctcatgtgga
    13621 ccgaaaacga agatgcaaaa aaagaatatg aggaagggct caatagtaaa tcaaaagatt
    13681 attcattagc attacaacat ttcagacagg ccattatgct tgatagcgct ttcctttccc
    13741 cggtatttga aataggcatg tattattaca cgcaaaacca gtaccgtgat gcgctgtcat
    13801 atttcaacca gattttgtac cgcataccgt tttacggtga actccagttg cttgtaggcg
    13861 aaatatattt tcaggaaaaa cgatacagtg aagctgcaac agcatttgag gcagcactgg
    13921 aatatgcgtt tattgataaa gatatgaaat actcaagcca gcttaaagtt gctactgcat
    13981 attataacat tggccagtat acaaaagccg aaactttttt acagcaggcc atatctacaa
    14041 aaaaaggcaa cctttcacca tatttattgc tatcagccat ccagatgcaa aagaatgatt
    14101 atgatggtgc tcttcaaaca ctgcaggctg catataaaat tgactccaat aaccctgaag
    14161 tactgtacca gctaggactt gttttttaca acaaaaatga caataaagcg tatgaatatt
    14221 ttgaaaaagt atacatgcaa tatcgacaga agcataaaga aatccctgcc aaatatatta
    14281 aggcattcat tataacagct cagtattatt acacaacaaa gcaatattcc aaggttgtag
    14341 aacttaccac aattgtacct gaaaaccttc gtgatgaatc gttatcattg aattatgcgc
    14401 gctcactgta tatgcttgct caatacaatg aagcgatagt tcatttcaat aaattcaggt
    14461 tatcagacaa tgacttattt atactctgca aatgctacgc ttttataaat gacacctcaa
    14521 aagttaaaga gatactgtcc agcaactatt acaacacaaa ttttattaat aaagttaaag
    14581 aagataaatt gctaaaatcc atctacacag aagttcaaaa gcaacgcgaa actcccgcaa
    14641 ctgttcaacc acaaccccag gaagaacaaa aggacctgcc tgcgacccat gaaactacta
    14701 cagaacccat acatccgcaa taatagtatt actataatac aggcgctgat tattgctacc
    14761 agtgtaattg ctctattcca tgtatttcat ttacttcata tcattattat tgcattcatt
    14821 gtatcacttg gattacaagt atggtgtcag ctcaatccct gttatgctga ttacattacc
    14881 agtgccgaag cacctcttgc ttcattagcg ttacttatta ctatgctcat ttcacattcc
    14941 atgtcattat caattgttgt tactttcatt gttcttttta tatatcatta ttttttttca
    15001 tcatatgaat tataccatga tatatacttt actgcattac tgttttctat aattctgatt
    15061 tttgttagtt tatacctgaa caacatgggt atttttaatg attatactat caaaattttc
    15121 actggtgttc agagtaatac gtcattatca atagctatca tttcaatatt gatgagtata
    15181 ctatacacgt acctttcaaa attaaaatac gaatttagaa ttatcagttt agggcataac
    15241 tatataaatc tactgggaat agatgaaaat attattcatg gtatttcaag cattgcaaag
    15301 gctcttatct ctactatgat attcatcctt ataggatgga tgggatcttt gcttacaacc
    15361 ataccacttt tgcgcaaatc aagtcatcct ttgtatatta tgatatttac agcaatgtta
    15421 tcattactgt tatatgcaat acatttcgta cacccatttt atataatcat tcctgttgta
    15481 ataattgact ttgtatatgt tttttttctt ggcaagaggt taccatgctc aggttaaaca
    15541 atctttcatt aataattacc gataatcata ttcttaaaaa tatcacagtt gaatttttcg
    15601 aaggtgaaat tattggtata attggcaaac caggaagtgg caaatctgtt cttttgcaaa
    15661 cgatagcaca gtactataca tactacaatg gcgaaatttt attaaacgca aaaccaatac
    15721 atttaatcaa aaaaagggat ctagctcaaa aaatttctta tcaacccgca ggttattttt
    15781 ataaccatta catcactctt tatgattacc ttgtatcaag cagaatacct tataaaactt
    15841 atctttcacc tttccaatct tttgattatg agattgtcga taaatatatg tatcattttg
    15901 aattacaaca atataaatct aaaccactga tgcttctttc ttctgatatt caggcacgtg
    15961 cccttttagc atttcatttc atacgagatg cacagatagt aatgctggat aatccaacac
    16021 aaaatctttc tttaccatca tataatcagc ttcatgatgc tattatacgc tattgttcaa
    16081 ccggaaagaa ttgctgtatc attgcttcac atgacattaa tttccttgct caaacagtag
    16141 ataaattata tattatggaa aacggctcta tagttgattc atgtgttcct gctgatttaa
    16201 ctgaagattt aataaaaaaa atatttggag tggatgtttt tattacacga aacatttata
    16261 acggcaaacc taatgtccat ctatattttg aatattaaca aagcacacca gatatcccgg
    16321 ggtagaatag atatgctaag ataatattaa atgtaataat ctaatttcct tatcccttct
    16381 tctacgctat tgattaaaag cagatcatat gactctgttg cattaattaa actttgaaga
    16441 ttatctgaag ctcccaaaat cataaccctg cgtttttttt gtcttgcaaa gtgcgacagg
    16501 tcagttaatg ccttgagccc tgatgaggtt accatcgata cacctgaaac attaataata
    16561 acattatttg tggcaaccag gctttctatc aaacttataa attctctttc agtggctgaa
    16621 cacaggaccc ctgctaagtt aactattttt gccccttcac gttcttctat tgaataattt
    16681 atttcagctg ccattatttg ccaaccattg ttaaataaaa gttcaatgct tcctttacca
    16741 atccatcatt cacttcacaa ccaatccgtg gtttaccaat tgattccaga agaacaaagc
    16801 gtgttttgcc ttttatattt tttttgtcgt acatcatatg attaacaatc ttgttagggt
    16861 caaatgaaat attgggcaac aggctatatg cagatataat atcccttata caatcaaaat
    16921 cagcttttgg catgccctcc ttcatggata tatacgattc aatcaacata ccagcagcaa
    16981 cagcatcacc atgacttact gtatattcaa gtagagattc tatagcatgt ccaactgtat
    17041 gcccaaaatt taaaatggct ctgatatttt tttcgtattc atccttctca accacagaag
    17101 ctttgaatcg tactgaacta ttaatacagc tatataacga attgtcaata tggccatatt
    17161 gtatttcttt attcagcaat aatgttaatg atgaactatc tccaattaac gcatgtttta
    17221 caacttctga catgccacag cgccattctg attgaggtaa tgtttgtaaa aaatcaatat
    17281 caataattat ataatcaggc tgatgaaaat ggcccactat gtttttgcct gctttaatat
    17341 taactgccac cttaccgcca atactggcat ccaccattga cagaagtgtt gtgggaacat
    17401 acactacatc tataccacgc atatatgctg atgcaacata cccggcaaaa tcagtggtaa
    17461 caccaccacc tatccctatt accagtgaat tccgggtaca ctggttttca agtaacaggt
    17521 tgaaatattg ttcagcatat gcatatgatt tattttgttc gctgtcatta taatgtattg
    17581 ggatagcatc aattaatgac aggctttggt caataacctc tttatgcaat gaatataccg
    17641 tatcacttac aattgcaaca atgacatccc actttttata tgggatcatt gatgataacg
    17701 aagccagtat gcccttgcca attattattg tagatttttt tgaatctaac tgtaaaacaa
    17761 gagtatcact atttgttatg ggatccacag tgaaccttta tatatccgtt cgtttttatt
    17821 ctgtaataat tgaataatat actcattaag tggcgggtac ataacaatat cagccaattc
    17881 actgacaggt ttgaaactga aatcatataa aattttctca tcacctaaac gtattgactt
    17941 atttaaaaga taacaccaga atacaatatt aataatatga cgtttcccat gaggttctat
    18001 tgagtcagct attaatgcaa tatcaccaac ttcaactgca agccccaatt cttcaaaaaa
    18061 ctcccgtttg agtgcctcat caagtgattc ctgatattca acaccacctc caggcaacaa
    18121 ccagtagatt tcccccccct tttttatgag ctatcatcaa tattttgttt tcttcaacaa
    18181 ctaatccagc tacacggatc ctgatctttt tatgtgatag ctcaaacctc atattgttat
    18241 cctgttggag tagataagtt ccagagcttt ttttgcagca tccgactctg cttttcgctt
    18301 actgctacct gttccaactg taatatcacg atcgtttata gttaatttaa tagtatacat
    18361 tttactatga tcgggaccag attcatggat cactgtatac aatggtctgc ttttatattt
    18421 cttctgtact aattcctgta atgttgtttt tggatcacgt aaatatgaaa gtttatcaat
    18481 acgctgaata tcttttttta ataacctcag taccaacttt tttgattttg ttaatcctgc
    18541 atcaagatat actgcaccaa taattgcttc cagtgcatct gcaagtattg attgacggta
    18601 tctgccacct gtaagctctt cgccccttcc cataataata tagtcaccta agttcatctg
    18661 attggcaact tttgccaggg tatcttctga aactacagca gattttattt ttgctaaatc
    18721 accttcgggg taattttcaa acgatttaaa tagatattca tttaccacta aacccaatac
    18781 tgaatcaccc aaatactcca atcgctcatt atcttttaca ggaattgagg attcattaac
    18841 ataggaacgg tgagttaaag cacgttttaa taatgattta tttgaaaagg taaagtgtaa
    18901 acgtttttca agctgttgca atttacgatt ttgccttttt ttatctaagc ccttcttatt
    18961 ttcatcatcc attgctcatc acaaaaaaac aaaattcaat cactgtaatt ttgcttcaat
    19021 atatttaaca acatcttcaa cggttaataa tttttcggca tcttcatcgg gtatttctat
    19081 tccaaactct tcttccagcg ccataaccag ttctacagtg tcaagggaat ccgctcctaa
    19141 gtcatcaaca aatgatgcct gtagtgtaac ttctgactca tcgaccccta actgatccac
    19201 aattattttt tttatttttt caaaatcaac tgtcattacc tctggccctc cttacctgac
    19261 ttgccttaca aattaaaccc aggacaacaa aaacatatat atgatagtag taaaaatatt
    19321 gatattttgt caagattaat ataaaaggag cgtctcaaac gctccatatt catacacaat
    19381 cattaaccct tgttaacttt tacaaccggt tccactataa gttttccctt ataatggcca
    19441 cattcggggc atactctatg aggcagaacg tatgcaccac aggttgggca aggtcttaag
    19501 tttggtgctc caatcgcatc atgcgaacgt cgcatccttg attttgcttt tgattttcgc
    19561 ctttttggta ccgccacttt ttttctcctt atttactatt atcaaaaatt tcattataag
    19621 ttcaaaaata gcacgttgct gtgtcaatgc ttttttaatg tgtagtatat taatttttct
    19681 tttcgttttc aattgcccct aaaaaaatac tgtgctgggc tgcctttttt gcaaaaaaag
    19741 actgtgggaa ctcagtggtt actctgttaa aatattcctg tgctttataa tactcacctt
    19801 taagagcata catcctgcca atactatata atatctggtc aatattaaag gcatctttat
    19861 actttgattc gcaaaggaga taataccgta aagcctcatc aatattatta agatattctg
    19921 atgacatgcc tgcctgctgt aatgccagtg aagcaaacaa cggcgatgca tattgtgcag
    19981 ctttttcata atatctggca gcattggcat agtctttttc attaaaataa aggcttgcaa
    20041 taatataata gccattgcgg tgaacatacc caaaataaga gctgtcagca attgtctgta
    20101 accttttaac agtgcgcaaa aacttttcat tatgttcttg tatatcggtt gtatacgttg
    20161 ttctatattc atcaagaatt gcttcaaaat cagcagtatc tttttttatt ttataatcag
    20221 catacacaat agctgcaata ccaagaacta aaaccactga cacaccaata gccactttaa
    20281 ttgccacaga acggtgagcg cgaatataat cgcggacatc catcagcatt tgctcaacaa
    20341 tattgcggtg aacagtaact tccctgtcta tcatgtgtta ttccttatcc agtttatttt
    20401 ttaaaagttc acccagtgta acggtaccag acgttgatgt gtttaaaatc ttcttgattt
    20461 cttccttttc ggacataatt tcataatgct tgatactgag tgacagtcgc ttcttatcaa
    20521 catcaacacc caatacaaca gcattcactt tatccccaac tttatagata gtatcaactg
    20581 attcaatttt ttttcttgat acttctgata tatgaacaag cccttcaacg gtatcgccat
    20641 caatcttaac gaacatacca aaaggaacaa tcccggtgac aattcctgaa acaatagttc
    20701 tgggaggata tttttcagca attacttccc atggtgattt ggtcagctgc ttgatgccac
    20761 aggcgatctt ctgttcctct ttattaattt caagaataat aaactgtatt tcctgaccct
    20821 ctttatactg tgactgaata tctttaaaat tttcatccca cgaaatatcc gatatatgga
    20881 tcaaaccatc gatatcaggc tcaagctcca caaacatacc aaaattaaca atctttttaa
    20941 ttggacgcgt atgaatactc ttcacaggga aacgctcttc aatagtctcc cagggatttg
    21001 gctgtaattg ctttattccc agtaataact ttctgttttc atcatctaca cttaaaacaa
    21061 ctgattgtat cctgtcgcct tttttataca gttcttttgg atttactgtt cgctttgccc
    21121 atgaaatgtc cgatgccgaa caataccctt caacaccagg ctcaatttca ataaacatac
    21181 caaaattggt aatggtgaca atttttcctt cgattgtgtt cccaggctga tatttttcag
    21241 gaaccgacaa cca
//
LOCUS       DAORYL010000054        20310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_2229_length_20310_cov_13.156406, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000054 DAORYL010000000
VERSION     DAORYL010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 20310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20310
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2229_length_20310_cov_13.156406"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..972)
                     /locus_tag="PLV81_10260"
     CDS             complement(73..972)
                     /locus_tag="PLV81_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10519.1"
                     /translation="MEKIEEKVNRLENALEEFTRTVGLEFNKVYNAFMLSRMEYERIS
                     QDIANQDKRIEATRELLETFIKESEKQRQEDRQKFNEFKDEMRAFKDEMRVFKDEMKE
                     FKDEMKEFKGEMKGFKDGVVAFQQEMKEFKDVMKDFKDDTLSFKEEMKQSFRKMNQQW
                     GELANKMGTIVEDIIYPATRPVLEKYFNCELETIMMNITRKKDGIKDEFDVIAVSEDK
                     VFLIEVKSTMRQQYVDDFKNKKIERFRFLFNEYNDKQLIPILATLRYEESMLNYVTNQ
                     NIYAMAYREWDYMDILNFDNISL"
     gene            complement(1316..2383)
                     /locus_tag="PLV81_10265"
     CDS             complement(1316..2383)
                     /locus_tag="PLV81_10265"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOM10520.1"
                     /translation="MNKIKVLVVDDSAVMRKTLTDILNGSSKIEVVGTALDPYIAVNK
                     IKQLNPDVLTLDIEMPRMDGLTFLRKLMLANPMPVIMVSAFTDKGALQTIQALEYGAV
                     DFILKPRSDDSAEWDRFASELREKVITAASIQSDRFTARSREKAIEVEEKFSADVILP
                     KTVSKVRTTHSDVIIALGASTGGTEVIAKILGSLHENVPAIAIVQHMPEKFTEAFANR
                     VNNNSKLYVKEAQNGDRLYRGTALIAPGNRHMLVRSDASGYYVEINDGPPVNRHKPSV
                     DVLFRSVAQNVSKSVGILCTGMGADGAEGLGEILQAGGMTIAQDEVSSVVFGMPREAI
                     KRGAAQKVMNIEQMIAYINSL"
     gene            complement(2376..2942)
                     /locus_tag="PLV81_10270"
     CDS             complement(2376..2942)
                     /locus_tag="PLV81_10270"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOM10521.1"
                     /translation="MYIRNSHRLKKPMKVLYPGDCYVSNKDEYIGTLLGSCVAVCLYD
                     PVNKVAGMNHFVLPGKVVQAHTKQKKSNAGDEEKELLKYGTRSIEKLITQMEQYGKRE
                     NIVAKIFGGGKVLDYQTTQYGISNMNVRIAKILLEMADIPIVSEDVGGTVARKVMFDV
                     ETGRAYCKQLTKVEEKDKTFTMELSQFE"
     gene            complement(2978..3790)
                     /locus_tag="PLV81_10275"
     CDS             complement(2978..3790)
                     /locus_tag="PLV81_10275"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOM10522.1"
                     /translation="MNNPFYQQLDQQDFDYIRNIVYRESGIKLSEMKRALVQSRLLRR
                     MRELGMQRYDEYCDFLRENYDDEIENLINCITTNKTEFFREREHFSILQRIVEQELSH
                     KNIAIWSAGCSTGEEAYSIAIVLCESNFKKQFTIIATDIDTKVLETARSGIYPLEVVQ
                     TLDISLLKKYFLKGKGQYDGYAKVKNFLKEKISFQHLNLLDDNYRLKGHFDVIFCRNV
                     MIYFDKDVQVKVLKKFYDYLPKGGYLFLGYAEAIVSANLPFHYVAHSVYQKR"
     gene            complement(3794..4357)
                     /locus_tag="PLV81_10280"
     CDS             complement(3794..4357)
                     /locus_tag="PLV81_10280"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10523.1"
                     /translation="MIRVFDATIKQPVVILVPGEYFAAENQVLSTIVGSCFVVCLYDS
                     IKKIGGMGHCLLPVLSSLQMKKEDIFNYNINFMEHIIGQMVKLGADRKNFIVKVFGGT
                     GSSYNQKFTTDFISEYCKNENMSLDAIDIGGNYRRKLYFFCDTGKVHRYLVEANDSIS
                     EFIKMEKEFIDKVLTDKVQYGNVILFE"
     gene            complement(4382..4873)
                     /locus_tag="PLV81_10285"
     CDS             complement(4382..4873)
                     /locus_tag="PLV81_10285"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOM10524.1"
                     /translation="MNIENTAIENKPDTASTEQFVTFIIGDETYGIEVLKVQEIIGMT
                     HITHVPNTLSFMKGVINLRGSVVPVIDMRKKIGMEEIEYNAFTVIIITEVKGKLIGMI
                     VDSVQDVVTIPVQKIQDTMRFTSHVSTDYIKGIGQIDDNLVIILDVDKLLTSEELAHI
                     KTT"
     gene            complement(4885..5256)
                     /locus_tag="PLV81_10290"
     CDS             complement(4885..5256)
                     /locus_tag="PLV81_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM10525.1"
                     /translation="MKHIVIIDDSPTIRTSVEYTLKDSGYGMVHAENGVDALNKIQDL
                     LSKGEEIALCIVDINMPQMDGITFIKKFRENDKFTPVVVLTTEAEEEKIQEGKKAGAS
                     GWMIKPFKAEQLQSVVHKFLR"
     gene            complement(5379..5633)
                     /locus_tag="PLV81_10295"
     CDS             complement(5379..5633)
                     /locus_tag="PLV81_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOM10526.1"
                     /translation="MYIIEVDDFVNVKHIKQFADEVMTVLRTEKDIVIDFTKSKRLDL
                     SVVQVILSLLKTARQLNKTVKFKGVNDTVKKQLKLCGVIR"
     gene            complement(5800..7524)
                     /locus_tag="PLV81_10300"
     CDS             complement(5800..7524)
                     /locus_tag="PLV81_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10527.1"
                     /translation="MMNVIAYEQSIRGHYNYLKDLKGNTSTLFSELIQRFTVINMRID
                     EFFTMIRPYIYYFYNTDENLPDTFLHDVIIGTNNEMNEYIDTIVKYMIADRETNKEVE
                     NAINKTLTLSNSVAMILDAVEAIDLYAKNTMIISIKAGEEGSTLTTIAVEMSHLADMV
                     NDVSSQFQEIIEYLDKLRTKFSNTCQTVDIIIENYLTHLQIKMNSTIREIIGHQKKLS
                     YNVESIVNFADQLKSSIHKILQEFQVEDIFRQDIEKILFAIEALEEGGIDDSEKDILF
                     WGMYQKLLSLQHDFMHLYTSAGESLHTMKSNVEAIASDYTKASETVFEGDEFAIEKVY
                     NSLEMLQHETIQYIEDILAKKASLLEISSDVLEQLHKFQEFFTKIQDVVRKFDVVNML
                     TRIELARHQQLQKTIASSLSDISIMPKKIKKIVEESENLYKDVIGTMDATYQSYKDAM
                     SIQDSILNNCASNLKKISLKLYESKKYYIDISQQIERNVNALQQFLSEKSEQIEAFKN
                     IEENIKLFCDMLDGAGVEKNMPDVEKINKAVDTLTMLYGTDYKGLMLVSLLKEYSAAK
                     TEDTVIVF"
     gene            complement(7549..9633)
                     /locus_tag="PLV81_10305"
     CDS             complement(7549..9633)
                     /locus_tag="PLV81_10305"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOM10528.1"
                     /translation="MADDAIKQVFLAETKELLENLENDIVRYEETKDPELIHSIFRYV
                     HTLKGGSGMAGYEDVYQFTHALENILDSVRNGVLPMNDLLADIVFASIDVIRLRLYSE
                     EDPSKFEEKFALLQAKINDIESILQVKEEKTIEEDKTEEIQPVYNFFRVKAKFREDIF
                     KSGIDPLIIMEDLASLGKITELRVNRNDVPLLENLDPEKCYLSWDVILKTKSKEEALD
                     DVFIFVRDDNEISITNVTKNYVAQPVVEDVPKLGELLLSKGMLTEKEYDDVLSEHEKT
                     KKKVGEIAVTKGYVEDNDISLALKEQENIKKHLSTSTLRVDAKKVDNLMNLLGEMVIG
                     FSGIKRIAEEIEDEKAYRLNNALYGVDRIIREFQEHLMRIRMVPIGPVFEQFRRFIRD
                     TAKSHGKLIHLEIAGGDTEIDKTVIERINDPLKHLIRNAIDHGIESPQQRQAKGKPQE
                     GTILLKAYHQEGNIFIEISDDGQGIDREKVLQKVMASGISSKLDDLPDAKIYEFLFAP
                     GFSTAESVGDLSGRGVGLDVVKTNIEALRGSVEVFSEADKGTTFRIKLPLTLAIIDGM
                     LVKVAHETFIIPILSIIESVKPGKKDIKTVENKGEVVHVRGEYVPLVRLYKEFKLPQT
                     IENPWDGLVVIVESEGKALGLLVDELVGQQQIVIKSLDNQITSSRAISGAAILGDGTI
                     ALIIDVHGFISS"
     gene            complement(9657..11705)
                     /locus_tag="PLV81_10310"
     CDS             complement(9657..11705)
                     /locus_tag="PLV81_10310"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOM10529.1"
                     /translation="MKLFEGKFNSLKVFLSAIVGTIAAIAIIVLVITSYNAAYNALEK
                     SYINQIKNINDSINFIMKGYFEGQIQVAKLHASRNAVRDALLSGDYRQVTKDLEKFLT
                     ENKNLYENIFIATAERDPVILAAGTPAAVGIKYRAAGYEKAIDEALEGKIGLSKPNKS
                     PVSGLPVILIQVPIKHEGRIIGLFGLPLELGKYTYEIVKDIKIGETGYPAVADLEGIT
                     FAHPDKEQILKLDLSKLDFGQELLKSPDRTVIRYTWQGKDKIMIGVRNKEYGYIVMST
                     MFLSDVSDSARSMAGVMVLFGLIMIAGAVIGIYYIIAMRLNPLERLKEIAGALAGGNV
                     TMRYEGKLYNDEVGDMAKAINNTMDNLERLVAEVKVAVSNLTQAVNEIANGNENLSQR
                     TSEQASSLEEIAATIEEATATIKQNAEHATRANQIAEKTSELAKNGNNLVSDAVVAIN
                     EINASSKRIGEILTLINEISFQTNLLALNAAVEAARAGEQGRGFAVVAGEVRNLAQRS
                     GSAAKEIGELIRDSLEKIDTGTTLVNKSGEALKEIMQSIQELYKTITEIATASDEQKQ
                     GIDQINVAVSDLDTMTQQNAALVEETASASEEMANQAQELASMMERFVIREEISEKVF
                     GSKHRELHLHAAEVGKKVGVKRVEKPKAKEEKKEATTSEEKKEKGPNNKLIDDGFEEF
                     "
     gene            complement(11813..12778)
                     /locus_tag="PLV81_10315"
     CDS             complement(11813..12778)
                     /locus_tag="PLV81_10315"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HOM10530.1"
                     /translation="MMIFVIAICLIITHCHASKQDYRQLMRQLVISISKEAKKHNPEF
                     IVIGQNALELVTVNGKPNGKVSKDYINALDGTGIEELFYGYENHDGIKTPKEVTQYFL
                     SYLKIFSQHKKPVLVIDYVKTKNQAQDSYKRSSQKGFISFQTNRACSIIPQWMFNKNE
                     DPVTTLTQAKNFLYIINPEKFQSKDEFVQALSHTWYDVLIVDAFFWDELLTKLDVECL
                     QHKPNGARRLVIAYMSVGEAEDYRYYWKREWNKSKPAFLEKENPDWKGNFKVRYWNKE
                     WHTILYGAGDSEKFIESYIGKIVYTNYDGVYLDVLDAAMYYEIKK"
     gene            13226..13651
                     /locus_tag="PLV81_10320"
     CDS             13226..13651
                     /locus_tag="PLV81_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10531.1"
                     /translation="MYVLFIIIVLAIIILVWFIISNFITLVLFGWNSLSKLLVETPEK
                     QQPVQKYYLQFIFIQHEYRTRGFVTLSIYSQGIQIKAPLLPQTMVKLLFIPWEHIQKV
                     HNRKILWFNVLQVETASIQLYILGKSALHIEKYYKAIAH"
     gene            complement(13698..13880)
                     /locus_tag="PLV81_10325"
     CDS             complement(13698..13880)
                     /locus_tag="PLV81_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOM10532.1"
                     /translation="MPVYEFKCNDCNTTFSELRKMGDTKAPQCPYCHSTNTEKIFSVF
                     ASSSVKTSCSTTSGGG"
     gene            complement(14086..14928)
                     /locus_tag="PLV81_10330"
     CDS             complement(14086..14928)
                     /locus_tag="PLV81_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10533.1"
                     /translation="MYPSEVTGPGKLRVYVQVTKQERKGGVKVTLVDVRAFDKMPKDL
                     WEEWMEFEDKYVPLKILTVRNMKYAFDSLKKAVDNVLGKKEKPPKWFYGSDNASLNNN
                     ARIEHVIDDAERMATEGKYVVMIRNDNNFVVEGNILIDFPGEIFDIERDLWEKNPHKP
                     DLLIEDITLARGNKILVTVKKDKGFFPDALWKQKGSKAVVLRVTVGDKVIEAKLPEFD
                     PKRNLKYGEVQFTVPDVTITEETTVAAFIDATNQIAERSKGNNKKTVTLNPKEQQGGA
                     RQKK"
     gene            15164..15955
                     /locus_tag="PLV81_10335"
     CDS             15164..15955
                     /locus_tag="PLV81_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOM10534.1"
                     /translation="MDLQLTGKVAVITAASKGLGKAVALELSKEGAVCYICSRDSNLL
                     QKAADEIMSVTGNTVIALPCDVTNPDDITKCKDAIVKDYKTVDILFTNAGGPPAGFIS
                     DFSSEDYIRAIELNLISAINLIYAFLPVMQKNQYGRIIASTSITVKQPLDNLVLSNVS
                     RVGVVAFIKSIANQYGKYNITANAVAPGYILTDRLKSLIELKAQQNNITYEKALASFA
                     SEIPLQRIGTPQEFAALVTFLASPKSGYINGQTILIDGGMYKGLF"
     gene            complement(16066..16836)
                     /locus_tag="PLV81_10340"
     CDS             complement(16066..16836)
                     /locus_tag="PLV81_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOM10535.1"
                     /translation="MKLKLISWNVNGIRAVYKKGFFQWFVSEKPDILCLQETKATADQ
                     FPEELKKTDGYYTYFAEAQKKGYSGVALFSTVQPKKVETGFGIERFDTEGRILIAYYD
                     AFVLYNIYFPNGKASKERLQYKMDFYDAFLVHAEKLRKKGMSIVMCGDVNTAHTEIDL
                     ARPKENENVSGFLPEERAWIDTFINKGYTDTFRLFIKEGGHYSWWDYKTKARERNIGW
                     RIDYFFVSNDLVPKVKDAFILDKVEGSDHCPVGIVLEI"
     gene            complement(16877..17200)
                     /locus_tag="PLV81_10345"
     CDS             complement(16877..17200)
                     /locus_tag="PLV81_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020503156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10536.1"
                     /translation="MALEQKDIEFIESYLKENLPRILVEQSLAKPPVVYEIELRERMV
                     RVEEELKHQRELMKEGFDQINKRLELLENRFDVLSRRLFQFMVWTFGLVVSSTGIIIA
                     FMKMQ"
     gene            complement(17221..17649)
                     /locus_tag="PLV81_10350"
     CDS             complement(17221..17649)
                     /locus_tag="PLV81_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019951681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10537.1"
                     /translation="MALEQKDIEFIESYLKENLPRILVEQSLAKPPVVYEIELRERMV
                     RVEEELKHQRELMQKGFELMEKSFQMMERRFEMIDKRFEQVDKRFEQVDKRFEQVDKR
                     LELLENRFDQLSRRLFQFMIWTFGMIVSATGIIIAFMKIH"
     gene            complement(17766..18938)
                     /locus_tag="PLV81_10355"
     CDS             complement(17766..18938)
                     /locus_tag="PLV81_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOM10538.1"
                     /translation="MTDIKKRAYQFIILFGIISLLGDIIYEGARSVNAQYLKELGANA
                     LWVGVVAGLGEFIGYAIRLVSGYLSDKTKAYWLFTFIGYGLLISVPLLALTGLWQVAA
                     LLMVAERLGKALRSPAKDTILSQVTTQVGRGLGFGLHEAMDQIGAIAGPLIFTAIFAY
                     IVDYNLGYTIMWIPFMLLIAVLTLARVLVPHPETFETKATGQSGPEKLTSTFWLYTAF
                     SAFAIIGFASFPIIAYHLKSHTIVSDMSIPLLYAIAMGVDAVFAIVIGKLYDTKGMKV
                     LFIIPVASAIVPVLSFAFNWIAVTIGIILWGLVMAIHETIMRAAIADLTSLKKRGTGY
                     GIFNTAYGVCMFFGASIMGWLYEIDVQYIMIFAFVSELIALILFIYIMRSIQVKSK"
     gene            complement(18938..20083)
                     /locus_tag="PLV81_10360"
     CDS             complement(18938..20083)
                     /locus_tag="PLV81_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOM10539.1"
                     /translation="MAKTIASLLSLLLISNFVPQRSTQGKEYIICGNNTQQKIYYATS
                     SNFSKNHKGEKALDDDETTSWISAKGDDQWIEIDFGVKRIITDIVITAGTKDNYNTIT
                     YCILQFMYDGKWFDYSRVNFIQKDKKGFFGDSTYQRTVKVNVKGIDASTFRILIPHDA
                     TIDGYAAIAEIAVYAGSAKLQYYDKRLYNLCFPIKNGLFPENDSGYPNAPRGYRGGTH
                     YGLDIFYYFKEDNFLPIPVDETTPVLSCADGVVVRADIDYVPPTEEEWKSLSQYYQKN
                     PATFIKRSFGGRQVWIDHGNGILSTYNHLSKIDGKINTGVRVKKGQRIGWVGNSGLLG
                     EAQGQKWGQHLHFELWVDGIYLGYNMSLVDIKRYLRWIFAIRDMEEN"
BASE COUNT         6035 a         4427 c         2972 g         6876 t
ORIGIN      
        1 aatgttaaca tttcccatcg tagtatggga actatcgtat tatcacaata agttttatcg
       61 tggattcatt ttttataaag aaatgttatc aaaattaagt atatccatat aatcccattc
      121 acgataagcc atagcatata tattttgatt ggttacataa ttaagcatac tttcttcata
      181 cctgagagta gcaagtattg gtattaattg cttatcatta tactcattaa ataaaaatct
      241 aaaccgttca atcttcttat ttttaaaatc atcaacatat tgttgccgca tcgtagactt
      301 cacttcaata agaaaaacct tatcttcact gactgcaata acatcaaact catcttttat
      361 accatctttt ttacgggtaa tattcatcat tatggtttct aattcacagt tgaaatattt
      421 ctctaataca ggtcttgttg cagggtatat tatatcctcc acaatagtgc ccatcttatt
      481 tgccagttcg ccccactgtt gattcatttt cctgaaagac tgcttcattt cttctttgaa
      541 agatagcgta tcatctttga agtctttcat tacatcctta aactccttca tctcctgctg
      601 aaatgcaact acaccatcct taaacccctt catttcaccc ttgaattctt tcatctcatc
      661 tttaaattcc ttcatctcat ctttgaaaac tctcatctcg tctttaaatg cccgcatctc
      721 atctttaaat tcattaaact tctgcctatc ctcctgtcgt tgtttttcag attctttaat
      781 aaaagtctca agaagttctc ttgttgcttc tatcctctta tcctgattgg caatatcctg
      841 tgatatccgt tcatactcca tacggcttaa cataaaagca ttatatacct tgttaaattc
      901 aaggcctaca gttcgcgtaa attcctcaag agcattttcc agcctgttca ctttttcctc
      961 aatcttttcc ataaattaca atctccttcc cttaaagtat gctatgcttt ttatagtata
     1021 aatataaaat cacattatct aaaatgcaac caatatttta ataactggaa aataattttg
     1081 ggatagtcag caaatattgt aaatagtaat ttttagacgg ctgattaaaa cctggtaaaa
     1141 tttaacctct gaccgctccc gacgtgatca ggtgccatgt cttgatcaga ataatcaatt
     1201 attaaccaaa aaccaatgaa ttgtataaca gtgtcatgaa aacattgtgc aaaatgttat
     1261 gttatttgcc atatatccat acctatctaa cataaccttg taccatatta tgcttttaca
     1321 gactatttat atacgcaatc atctgttcaa tattcatcac tttttgtgcc gcacctcgct
     1381 tgatggcctc acgcggcata ccaaatacaa ctgatgatac ttcatcctga gctattgtca
     1441 tgccacctgc ctgcaatatt tcaccaagtc cttccgcacc atcagcaccc ataccggtac
     1501 atagtatccc tacactttta ctcacattct gtgctactga acgaaataac acatccactg
     1561 atggtttatg ccggttgaca ggtggtccat cattaatttc cacgtaatat ccactggcat
     1621 cagaccgcac cagcatgtgg cggtttcctg gtgcaatgag ggctgtgcct ctgtacagcc
     1681 tgtcaccatt ttgagcttct tttacataca gctttgagtt gttgttaacc ctgttggcaa
     1741 atgcttcagt aaatttctca ggcatatgct gtacaatagc aatagccggt acattttcat
     1801 gaaggctacc taaaattttt gcaataacct cggttccacc tgttgatgca cccagagcaa
     1861 tgataacatc actgtgggtg gtccgcacct ttgatactgt ttttggcaaa attacatcag
     1921 cactaaattt ttcttcaacc tcaatagcct tttctctact acgtgctgta aatctatccg
     1981 attgtatgct tgcagcggta ataacctttt cacgcaattc acttgcaaat ctgtcccatt
     2041 ctgcactatc atcagagcgc ggctttaaaa taaaatcaac agcaccatat tcaagcgcct
     2101 gtattgtttg caacgcacct ttatcagtga atgcgctgac cataattacc ggcattgggt
     2161 ttgctaacat gagctttcgc aaaaaagtaa ggccatccat gcgcggcatt tcaatatcaa
     2221 gggttaatac atcaggattg agctgcttta ttttattaac agcaatatag gggtcaagag
     2281 cagtaccaac aacttcaatt ttactggatc cattaagaat atcggttaat gttttgcgca
     2341 ttaccgcaga atcatcaacc actaaaactt ttattttatt caaactgaga taactccata
     2401 gtgaaagttt tatctttttc ttcaaccttt gttaattgct tacaatatgc tcttcctgtt
     2461 tcaacatcaa acatgacttt acgagcaacg gtgccaccaa catcttcgct tacaatagga
     2521 atatcggcca tttccagtaa aatctttgca attcttacat tcatgttgga aatgccatat
     2581 tgcgtggttt gataatctaa aacttttcct ccaccaaaaa tcttagctac tatattttct
     2641 cttttaccat attgttccat ctgagtaatg agcttttcaa ttgaccttgt gccatatttt
     2701 aacagttctt tttcttcatc accagcattt gattttttct gctttgtatg agcctgcaca
     2761 acctttcccg gcaaaacaaa atgattcatc ccggccacct tatttacagg atcatacagg
     2821 catactgcta cacacgaccc aagcaacgtt ccaatatatt catctttatt gctaacataa
     2881 cagtcaccag gatataaaac tttcattggc ttcttcaacc tgtgtgaatt gcgaatatac
     2941 atggctacct ctattaacac taacacagca ctatgagtta tcgtttttga tataccgaat
     3001 gggctacata atgaaaagga agattggcgc ttactatcgc ttcagcatat cctaagaaaa
     3061 gatagccacc ttttggtaaa taatcataaa atttttttaa tactttgacc tgaacatctt
     3121 tatcaaaata aatcatgacg ttgcggcaaa atataacatc aaaatgccct ttaaggcgat
     3181 agttatcatc aagtaaattc aggtgttgaa atgaaatttt ttctttgaga aaatttttta
     3241 cttttgcata accatcatac tggcctttgc cttttaaaaa atattttttt aatagactta
     3301 tatcaagtgt ttgtacaacc tccagcgggt atatgccact tcgtgctgtt tctaacactt
     3361 tagtgtcaat atcagtggca attatagtaa attgtttctt gaagttactt tcacataaaa
     3421 caatagcaat actgtatgct tcttcaccgg ttgagcagcc agcactccat attgcaatat
     3481 ttttatgcga tagttcctgt tcaacaattc gttgcaatat agaaaaatgt tctcgttccc
     3541 tgaaaaactc tgttttattg gtagtgatgc aatttataag gttttctatt tcatcatcat
     3601 aattttctct gaggaagtca caatactcat catagcgttg catacctaac tcacgcattc
     3661 tgcgtagcaa acgcgattgc accagtgcac gtttcatttc agaaagcttt atgcctgatt
     3721 ctctatacac aatatttctg atgtaatcaa aatcttgctg gtctaattgt tggtaaaaag
     3781 ggttattcat atattattca aataatataa cattgccata ttgaacttta tcagttaata
     3841 ctttatcaat aaattccttt tccattttta taaattctga aattgagtca ttggcttcaa
     3901 ctaaataccg gtgaacttta cctgtgtcac aaaaaaaata aagtttacgg cgatagttac
     3961 ccccaatatc aatcgcatca agagacatat tttcattttt acagtactca gaaataaaat
     4021 cggtagtaaa tttttgattg tatgatgacc ctgtcccacc aaataccttt acaataaaat
     4081 tctttctatc agcccctaac ttgaccatct ggccaataat atgctccata aaattaatat
     4141 tataattgaa tatatcctct tttttcatct gtaatgaaga taataccggc aataaacagt
     4201 gccccattcc accaattttt tttatagagt catacaggca tacaacaaaa caactaccaa
     4261 ctatcgtaga aagaacctga ttttctgcag caaaatattc accaggcacc agaattacca
     4321 ctggctgttt aatggttgca tcaaataccc gtatcatccc atcatacctg tttgcattaa
     4381 tttatgttgt tttaatatga gccaattcct cacttgttaa cagcttatcg acatccagaa
     4441 taatgactaa attgtcgtcg atctgtccaa tccccttaat ataatctgtt gacacatgcg
     4501 atgtaaatcg catggtatcc tgaattttct gcactggaat agtaaccaca tcctgaactg
     4561 aatcaactat catccctata agtttccctt taacctcagt aataattata acagtgaagg
     4621 cattatattc tatctcctcc atccctattt ttttccgcat atctattacc ggcaccaccg
     4681 aacctctgag gttaatgaca cctttcataa atgaaagcgt attaggaaca tgagtaatgt
     4741 gggtcatccc aataatttcc tgcactttaa gaacttctat accataggtt tcatccccaa
     4801 taatgaaggt aacaaattgt tctgttgaag cagtatcagg cttgttttct atagctgtat
     4861 tttcaatatt catatattcc ccccttatcg caaaaattta tggaccactg actgaagctg
     4921 ctcagcctta aatggcttaa tcatccaccc tgaagcacct gcttttttgc cttcctgtat
     4981 tttttcttct tcagcttctg tagtaagcac cacaaccggt gtaaatttat cattttccct
     5041 gaacttttta ataaacgtaa taccatccat ctgtggcata ttaatatcca caatacacag
     5101 ggcaatttcc tcacctttgg aaagtaaatc ctgtatttta ttcaatgcat caacaccatt
     5161 ttcagcatga accataccat atcctgaatc tttgagtgta tattcaacac ttgtacgtat
     5221 agttggcgag tcatcaatga ttacaatatg cttcatataa agctcctctt aaaaaagatt
     5281 gtatttgact ttgcatccct attaattagc cagaaccgct ttattaatcc cccgtgttat
     5341 tataatatac aattcataat aatacgtaac aacaaaaatt aacgtataac gccacatagc
     5401 tttaattgct tttttacggt atcattaact cccttaaatt taactgtttt attcaattgc
     5461 cgggctgttt tcaataatga taaaataacc tgcacaaccg acaaatctaa ccgttttgat
     5521 ttagtaaaat caatgacaat atctttctca gtccgcaata ctgtcatcac ctcatctgca
     5581 aactgcttta tgtgtttaac atttacaaaa tcatcaactt ctattatata catgtattgc
     5641 cccctttatg cactgacatc atcactgtca tactgaatct ggtttaacat cgcactatta
     5701 cctacttgaa aataagtttg gcatatattc cagtatgact cttcaacttt cataattcta
     5761 tcaatctgaa ccttatgcaa tacccctgtt gcacattaat taaaaaacta taaccgtgtc
     5821 ctctgttttt gcagccgaat attcttttaa aagtgaaaca agcatcaacc ctttgtaatc
     5881 tgtgccatat agcattgtaa gtgtatcaac ggctttatta atcttttcaa catcaggcat
     5941 atttttttca actcctgcac catcaagcat atcgcaaaac agctttatat tctcttcaat
     6001 atttttgaat gcttctatct gttctgattt ttcgcttaaa aattgctgca aagcatttac
     6061 attccgttct atctgctgtg atatatcaat gtaatatttc ttggattcat acagcttaag
     6121 agaaatcttc tttaaattac tagcacaatt atttaaaatt gaatcttgaa tggacattgc
     6181 atccttatat gactgatagg ttgcatccat ggtgccaatg acatccttgt ataaattttc
     6241 tgattcttcc actattttct taattttctt tggcataatg cttatatcac tgagcgaact
     6301 ggctatggtt ttctgcaact gctgatgtcg tgcaagttct atccgtgtta acatattcac
     6361 cacatcaaac ttccgcacca catcctgtat tttagtaaaa aactcctgaa acttatgtaa
     6421 ttgctccaat acatcactgg atatttcaag caatgaagct ttcttggcaa gaatatcttc
     6481 tatgtactga atagtttcat gttgtaacat ttccagtgaa ttatatactt tttcaatagc
     6541 aaactcatca ccttcaaaca ccgtttcaga agcttttgta taatcgcttg ctatagcttc
     6601 aacattactt ttcattgtat gcaggctttc ccctgcactg gtatataaat gcataaagtc
     6661 atgctgaagc gatagtaact tttgatacat cccccaaaaa aggatatcct tttcactgtc
     6721 atcaatacca ccttcttcca gcgcttcaat agcaaataat attttttcaa tatcctggcg
     6781 gaatatatcc tcaacctgaa attcctgtaa tattttgtga atactgcttt ttaactgatc
     6841 agcaaaatta acaattgact caacattata tgacagcttc ttttgatggc caataatttc
     6901 acgtattgtg ctgttcatct taatctgcaa atgtgtgaga tagttttcta taataatgtc
     6961 aactgtctga catgtgttgc taaattttgt gcgtaattta tccagatatt ctataatttc
     7021 ctgaaattga cttgaaacat cattaaccat atcagctaaa tgtgacatct ccactgcaat
     7081 ggttgtaagc gtagagcctt cttcgccagc cttaatggaa attatcatgg tgttttttgc
     7141 atacagatca attgcttcca ctgcatccag tatcatagca acgctgttac tcaaagtaag
     7201 agttttatta attgcattct ccacttcctt attggtttct ctatcagcta tcatatattt
     7261 aacaatggta tcaatatact cattcatttc attgttggta ccaattatga catcatgcaa
     7321 aaaagtatca ggaaggtttt catcagtgtt ataaaaataa taaatatagg gacgaatcat
     7381 ggtgaagaat tcatcaatcc gcatattaat tacagtaaag cgctgtatca gttcagaaaa
     7441 cagagtactg gtattacctt ttaagtcttt aaggtaatta taatgacctc tgatggattg
     7501 ttcataggca attacattca tcatatcgtg ttagtcctga actatgcatt acgaacttat
     7561 aaaaccatgt acatcaataa tgagggcgat agttccatca cctaatattg cggcaccgga
     7621 tattgctctt gatgaagtta tttgattatc cagactcttt atcacaatct gttgctgacc
     7681 aacaagttca tcaacgagta gtcccagagc ctttccttct gattcaacta taaccacaag
     7741 gccatcccat ggattttcaa tagtttgtgg cagcttaaat tctttataaa gcctgactaa
     7801 cggaacatat tctcctctga catggacaac ttcgcccttg ttttcaacag tttttatgtc
     7861 ttttttccca ggcttgacac tttcaattat cgaaagtatt gggataataa atgtttcatg
     7921 cgccactttc accagcatac catctattat tgccagtgtc aaaggaagct ttattctaaa
     7981 tgttgtgcct ttatctgctt cgctgaagac ttcaacactg ccacgcaatg cttcaatgtt
     8041 tgttttgacc acatccaggc caacacccct tccggaaagg tcaccaactg attcggcagt
     8101 agaaaaaccg ggtgcaaaca aaaattcata tatttttgca tctggcaaat catcaagttt
     8161 acttgaaatt cctgatgcca taactttttg taataccttt tctctatcaa taccctgtcc
     8221 atcatcactt atctcaataa aaatatttcc ttcctgatga tacgctttca gcaatattgt
     8281 accttcctga ggcttccctt tagcctgacg ttgctgagga ctttctattc catgatctat
     8341 cgcattacga atcaaatgct ttagaggatc attgatgcgt tcaatgacag ttttatcaat
     8401 ttctgtatca ccacctgcaa tttccaggtg tatcaacttg ccatgcgatt ttgcagtatc
     8461 acggataaaa cgcctgaatt gctcaaacac cggcccaata ggaaccatgc gaattcgcat
     8521 aagatgttcc tgaaattcac ggattatccg gtcaacacca tacagggcgt tgtttaaacg
     8581 atatgccttt tcatcttcta tctcttcagc tatccgttta atccccgaaa aaccaatgac
     8641 catttcacct aaaaggttca tgaggttgtc aacttttttt gcatcgaccc tcaatgttga
     8701 agtactcaga tgctttttaa tattctcctg ttccttgagt gccagtgata tatcattgtc
     8761 ctccacatat cctttggtaa cagctatctc tccaactttt ttctttgtct tttcgtgctc
     8821 gctcaataca tcgtcatatt ccttttcagt aagcatgccc tttgacaata ataactcgcc
     8881 caatttgggg acatcctcta caactggctg tgcaacataa tttttggtta cattggttat
     8941 ggatatctca ttatcatcac gcacaaaaat aaatacatca tcaagggctt cttctttgct
     9001 tttggtttta aggataacat cccacgacag gtaacatttc tcaggatcaa gattttcaag
     9061 cagtggcaca tcatttcggt taacacgtaa ctcagtaatc ttccctaaag aagctaagtc
     9121 ttccatgata ataagcggat caatgcccga tttaaatata tcttctctga atttagcttt
     9181 aaccctgaaa aaattataca ctggctgtat ttcttcagtt ttatcttctt caatcgtttt
     9241 ttcttctttc acctgaagaa ttgattcaat atcattaatt tttgcctgta ataacgcaaa
     9301 cttttcctca aactttgatg gatcttcttc agaatacagg cgcaatctga ttacatcaat
     9361 gctggcaaac actatatccg ctaaaaggtc attcatggga agaacaccat tgcgcactga
     9421 atccagaata ttttccaggg catgagtaaa ttgatataca tcctcatacc ctgccatccc
     9481 cgaaccaccc tttaatgtat gcacatacct gaatattgaa tgaatcaatt cagggtcttt
     9541 tgtttcctca taacggacta tatcattttc taagttttcc agcagttctt ttgtttcagc
     9601 caaaaaaacc tgctttatgg catcatccgc catgtatttt cctccagtaa ctgttattaa
     9661 aactcttcaa acccatcatc tataagttta ttgttgggac ctttttcttt cttttcttct
     9721 gatgttgttg cttccttctt ttcctctttt gcctttggct tttccaccct ctttactccc
     9781 accttcttac ccacctctgc agcatgcaaa tgcaactccc tgtgcttact cccaaatact
     9841 ttttcactta tttcttcacg tatcacaaac cgctccatca tgcttgcaag ctcctgtgcc
     9901 tgatttgcca tctcctcact tgcgctggct gtctcttcca ccaacgctgc attctgctgc
     9961 gtcatggtat ctaaatccga tactgctaca ttgatctggt ctataccctg tttctgttcg
    10021 tcactggctg ttgctatctc ggttattgtc ttatacaatt cctgtattga ctgcataatc
    10081 tcttttaatg cttcaccgct cttatttacc agcgttgtgc ctgtatctat cttttccagt
    10141 gaatcgcgta taagctcacc aatctccttg gctgcactgc ccgaacgctg cgctaaatta
    10201 cgcacctcac ctgctaccac cgcaaaacca cgcccctgct ctcctgctcg tgctgcttca
    10261 actgctgcat tcagtgccag taaatttgtc tggaatgata tctcattgat aagggttaat
    10321 atctcgccaa tgcgcttact tgatgcattg atttcattta ttgccacaac agcatcactg
    10381 actaaattgt taccattttt ggcaagctcg ctcgtcttct cggcaatctg gtttgcccgt
    10441 gttgcatgct ctgcattctg ctttatggtt gctgttgcct cttctatggt tgctgctatc
    10501 tcttcaaggg atgatgcctg ctcacttgta cgctgcgaca ggttttcatt gccattggct
    10561 atctcattaa ctgcctgcgt tagattgctc accgccacct taacctctgc aaccagacgc
    10621 tccaggttat ccatggtatt atttatagcc tttgccatat caccaacttc atcgttatat
    10681 agcttacctt catagcgcat ggtcacattg ccaccagcaa gagcacctgc tatctcttta
    10741 agccgttcta atgggtttaa acgcattgca ataatgtagt aaatacctat tactgcacct
    10801 gctatcatga taagcccaaa tagcaccata actccagcca ttgaacgtgc agaatcactt
    10861 acgtccgata aaaacatcgt gctcattact atataaccat attccttatt gcgaactcca
    10921 atcattattt tatctttgcc ctgccaggta tacctgataa cagtgcggtc agggcttttt
    10981 aacaattcct gtccaaaatc aagttttgac agatccaatt tcagtatttg ctctttatca
    11041 ggatgtgcaa atgttatccc ttcaaggtca gctacagccg ggtagccagt ttcaccaatt
    11101 tttatatctt ttactatttc ataagtatat ttacccaact caagtggcag gccaaataaa
    11161 ccaattattc ttccttcatg ctttattggc acctgtataa gaattactgg caagccagat
    11221 actggtgatt tatttggctt gcttaagcca attttacctt ctaaggcttc atcgatagct
    11281 ttttcatagc ctgcagccct gtattttatg ccaacagccg ctggtgtacc tgcagcaagt
    11341 ataacaggat ccctttcagc tgtggctatg aatatatttt cataaagatt tttattctca
    11401 gttaaaaatt tttctaaatc tttagtaacc tgcctgtaat cgccactcaa cagtgcatca
    11461 cgcaccgcat tgcgtgaagc atgtaatttg gctacctgta tctgtccttc aaaataccct
    11521 ttcataataa aattaatgct atcattaatg tttttgattt gattaatata tgatttttcc
    11581 agcgcattat aggcagcatt gtatgatgta ataacaagta caatgattgc aatggctgca
    11641 atagtaccca caattgccga caaaaaaact ttaaggctat tgaatttacc ctcaaataac
    11701 ttcatatata ccctccttga cgttatacgt ttttaaacta ttttcccgta ataaataata
    11761 aatatttgtt atacacttat gatatatgaa agataaaact aaatcaacaa aattattttt
    11821 ttatctcata atacattgct gcatccagta catctaaata cacaccatca taatttgtat
    11881 atactatttt tcctatataa ctttctatga atttttcact atcacctgca ccatacagta
    11941 ttgtatgcca ttccttattc cagtagcgta ccttgaaatt acctttccag tcaggatttt
    12001 ctttttctaa aaatgcaggc ttgcttttgt tccattcacg tttccagtaa tagcggtaat
    12061 cctcagcctc accaactgac atatacgcaa taacaagccg gcgtgcacca tttggtttgt
    12121 gctgcaaaca ttcaacatca agtttagtca aaagctcatc ccaaaaaaac gcatcaacaa
    12181 taagcacatc ataccatgtg tgcgatagtg cctgtacaaa ttcatccttg ctctggaatt
    12241 tttcaggatt tattatatac aaaaaattct ttgcctgcgt aagcgttgta acagggtctt
    12301 cgtttttatt gaacatccac tgtggaataa ttgaacacgc acggtttgtc tgaaaagaga
    12361 taaacccctt ttgtgaagaa cgtttgtacg aatcctgtgc ctgatttttt gtttttacat
    12421 aatcaatgac cagcactggc tttttatgct gtgaaaatat ttttaaatac gacaaaaaat
    12481 actgtgtaac ttctttaggt gtttttatgc catcatgatt ttcatatccg taaaaaagct
    12541 cctcaatgcc tgttccatcc agtgcgttga tgtagtcttt tgatacttta ccattgggct
    12601 tcccatttac tgtaaccagt tcaagtgcat tttgcccaat gactataaat tcaggattat
    12661 gtttttttgc ttctttactt atagatataa caagctggcg cataagctgg cggtaatctt
    12721 gtttagacgc atgacaatgt gtgattatta aacatatagc tattacaaaa atcatcattc
    12781 tgcgcatccg gttacctcgc tatatatggg ataggtttta aaaggaatta attctacagt
    12841 aaatgttact gtgtggcaaa tattttttgt actggtgatt gtacgggtgt gttttttgct
    12901 gggaaaaagc aaaaataaat tgttattatt caaaacagca taaggctcgt gattacgaat
    12961 aaaaagaaac aaggttatta atctaacctg atgataatac catcgcaatg aaagaatgtt
    13021 attttttaat tcagcaaaaa ctcagtgaat aataattacg gaagagactg ttccccccaa
    13081 tgcaatagaa actcattttt gtattttgca atacataacc aggaattttt ttgattgaaa
    13141 tttttattat tatagtaaac gtatcaatta aaaatacctt tatgtatttc aaaattatta
    13201 taaatcttct caaagtagtt atcgtatgta tgtattattt attataatag ttttagcaat
    13261 tattatcctt gtgtggttta taatatctaa tttcatcaca ctggtattgt ttggctggaa
    13321 ttctctgtca aaattactgg ttgaaacccc tgaaaaacag caacccgtac aaaaatatta
    13381 tctacaattt atctttattc aacacgaata cagaaccaga ggatttgtca cgctaagtat
    13441 ctattctcag ggaatacaaa ttaaagcgcc actccttcca caaacgatgg taaaactgtt
    13501 atttattcca tgggaacata tacaaaaagt tcataatcgc aagattttgt ggtttaatgt
    13561 tttacaggta gaaacagcat ccatacaatt atatatatta ggcaaaagtg ctttgcatat
    13621 tgagaaatat tacaaagcaa ttgctcatta acagtaacta tcgcccaacc ttaccagact
    13681 ggtaagcatt atattcctca tccaccacct gatgtggttg aacatgatgt ttttacactg
    13741 ctgctagcaa atacagaaaa tatcttttca gtattggttg aatggcaata cgggcactgt
    13801 ggtgcttttg tgtcccccat ttttctgagc tcactgaatg ttgtattgca gtcattacat
    13861 ttgaattcat atactggcat aggaaaacct catgtaaaat attttctttt tagtaataaa
    13921 acaaagatga tttagtttat tattatatac ttaaatattt atatttcaat aaaaaactac
    13981 tatttagcca taacggattt cttaaaaaag ggaataggaa ggagattccc gcaggcaaac
    14041 tacccaccat tgcctgcggg aacatttttt aacgatgcat cgcaattatt ttttttgccg
    14101 cgcaccaccc tgttgttctt ttgggtttaa tgttacagtc tttttattat taccttttga
    14161 gcgctcggca atctgattag ttgcatcaat aaatgcagca actgttgttt cttcagtgat
    14221 tgtaacatca ggaacagtaa actgtacctc gccatacttt aaattacgct ttgggtcaaa
    14281 ttcaggtaat ttagcttcaa tgactttgtc accaactgtc actcgtaata caacagcctt
    14341 gctgcccttc tgtttccaca aagcatcagg gaaaaatcct ttatcttttt taactgtgac
    14401 aaggattttg ttgccacgcg caagtgttat atcctcaatc aaaagatcag gcttatgggg
    14461 attcttttcc cataaatccc gttcaatatc aaatatttcg ccaggaaaat ctataagtat
    14521 atttccctca acaacaaaat tattatcatt tcgtatcata acgacatatt tcccctcagt
    14581 ggccatacgc tcagcgtcat cgatgacgtg ttcaattcgt gcattattat taagcgaagc
    14641 attatcactg ccataaaacc acttgggtgg cttttctttt ttgcccagaa cattatctac
    14701 tgcttttttt aaactatcaa atgcatattt catatttcta acagttaata tttttaaagg
    14761 cacgtactta tcttcaaatt ccatccattc ttcccataga tcctttggca ttttatcaaa
    14821 tgctcgcaca tctaccagcg taactttaac acctcccttt ctttcctgtt tagttacctg
    14881 cacatacaca cgtaattttc caggtcctgt aacttctgat ggatacatta acgtatatgg
    14941 gtcaccgtca gttgctggat aagctgtaaa tttttgataa atgagaccct atttctcatc
    15001 cattgaataa acaagcgatg cgagtataaa aaggacaata acaacaaaaa aaactttacg
    15061 cttcatataa cctccattat ataatagtat ataataatca tttaaaagct tactgaatat
    15121 gttaaaaaag tcaaaacaaa ttatttttat aaaggagttt acaatggatt tacaactaac
    15181 cggcaaagtg gctgtaataa ctgcagcaag caaaggattg ggtaaagctg ttgcactgga
    15241 attatcaaaa gaaggtgcag tatgttatat atgctcgcgc gatagtaacc tattacaaaa
    15301 agctgcagat gaaataatgt cagtaactgg aaacaccgtc attgcacttc cgtgtgatgt
    15361 aaccaatcct gacgacatta ctaaatgtaa agatgccata gtaaaggatt acaagacagt
    15421 ggatatactc ttcaccaatg ctggaggacc accagcagga tttatcagtg atttttcatc
    15481 tgaagactat ataagggcta ttgaactgaa tttaataagt gccataaacc tcatctacgc
    15541 ttttttgcct gttatgcaaa aaaatcaata tggcaggatc attgcttcaa catctattac
    15601 agtaaagcaa ccgctggata atctggtttt atcaaatgta tcgcgtgttg gcgttgttgc
    15661 atttattaaa agcatcgcaa accaatatgg gaaatacaac atcacagcca atgcagttgc
    15721 tcctggatat atcctcactg accgattaaa aagcttgatt gaattgaaag cacagcagaa
    15781 caacataacg tatgaaaaag cccttgccag ttttgcttca gaaataccgt tgcagcgtat
    15841 tggtacccct caagaatttg ctgcactggt tacattttta gcttcaccaa aatcaggcta
    15901 tatcaatgga caaactattc ttattgatgg aggaatgtat aagggattgt tctaagatct
    15961 atgttctatg ttctatgttc tattattgtg attcatttta tatttaacgg tttaatttag
    16021 ctgctgatac tgtaatctaa atacagcaat atatgagtat tttatttaaa tttccaacac
    16081 tatgccaaca ggacaatggt ctgaaccttc tactttatca agaataaatg catcttttac
    16141 ctttggcaca aggtcattac ttacaaaaaa gtagtcaatt ctccagccta tattccgctc
    16201 tcgagctttt gtcttgtaat cccaccatga atagtgccca ccttctttta taaacaacct
    16261 gaatgtatca gtatatcctt tattaataaa tgtatctatc caggcacgtt cttcgggcaa
    16321 aaatcctgag acattttcat tttcttttgg acgggccagg tctatttcgg tgtgtgcagt
    16381 attaacatca ccacacatta caatactcat acccttttta cgcaattttt cagcatgcac
    16441 taagaaagca tcgtaaaagt ccattttata ttgcaatcgt tcttttgatg ctttgccatt
    16501 ggggaaatag atattgtaca gaacaaaggc atcatagtat gcaattaaaa tacgcccctc
    16561 agtatcaaaa cgctctatgc caaaacctgt ttccactttt tttggctgta ctgtagaaaa
    16621 caatgcaacc ccactgtatc cttttttctg ggcttcagca aaatacgtat aatatccatc
    16681 agtcttttta agttcttcag ggaattggtc tgcagttgct tttgtttcct gcaggcaaag
    16741 gatatcgggc ttttcagata caaaccactg aaaaaagcct tttttataaa ctgctctgat
    16801 tccattcaca ttccaggaaa taagttttag cttcatactg tcgtattact atttttttct
    16861 actcataatc tttttcttac tgcatcttca tgaatgcaat aataatacct gttgatgaaa
    16921 caacaagccc aaacgtccac accataaatt ggaataacct gcgtgataaa acatcaaaac
    16981 gattttctaa tagctctaat cgcttattta tttgatcaaa tccttctttc attaactcac
    17041 gctgatgttt cagctcttct tccacccgta ccatccgctc gcgcaactct atttcatata
    17101 ccactggcgg tttagccaaa ctctgctcta ctaatatcct tggtaaattt tcctttaaat
    17161 aactctctat aaattctatg tctttttgct ctaatgccat gttgttattc ctttactttc
    17221 ctaatggatt ttcataaacg cgataataat ccccgttgct gatacaatca tgccaaacgt
    17281 ccatatcata aactgaaata acctgcgtga aagctgatca aatctgtttt caagtaattc
    17341 caggcgttta tcaacctgct caaaccgttt atcaacctgt tcaaaccgtt tatcaacctg
    17401 ttcaaaccgt ttatcaatca tctcaaatcg tcgttccatc atctggaagc ttttttccat
    17461 taactcaaag cctttttgca ttaactcacg ctgatgtttc agctcctctt ccacccgtac
    17521 catccgctcg cgcaactcta tttcatatac cactggcggt ttagccaaac tctgctctac
    17581 taatatcctt ggtaaatttt cctttaaata actctctata aattctatgt ctttttgctc
    17641 taatgccatg ttgttattcc tttcaaatat atcccttact ctttgacttt aaaaatcaat
    17701 gctattatat acatgcaaac actataatga taatgtagta cacgattaca ataacgtcaa
    17761 cataattatt tactcttaac ctgtattgac cgcataatat atataaaaag aatcagcgca
    17821 ataagttcac taacaaatgc aaagatcata atatactgta catcaatttc atataaccat
    17881 cccattatgc tggctccaaa aaacatgcat acaccatacg ctgtattaaa tataccatag
    17941 ccagttcccc gtttcttaag tgaggttaaa tcggctattg ctgcacgcat gattgtttca
    18001 tgtattgcca tcaccagccc ccacagaata atgccgatag tcacagctat ccagttaaaa
    18061 gcaaaggaaa gcacaggaac tatcgccgaa gctactggaa ttataaataa tactttcatt
    18121 ccctttgtat catatagttt gccaataact atcgcaaata ctgcatctac acccattgct
    18181 attgcgtaca gcagtggaat gctcatatct gaaactatag tatgcgactt caaatgatac
    18241 gcaatgattg gaaagcttgc aaacccaata atggcaaacg cagaaaatgc tgtgtaaagc
    18301 cagaatgtgg atgtcaactt ttcaggacca ctttgccctg tggcctttgt ttcaaatgtt
    18361 tcaggatgag gcacaagcac cctggctaac gtcaacaccg ctataagaag cataaaggga
    18421 atccacatga ttgtgtaacc cagattgtaa tcaactatat aggcaaagat tgctgtaaag
    18481 atcaacggtc ctgcaatagc tcctatctgg tccattgctt catgcagtcc aaaacccaaa
    18541 ccacgcccaa cctgcgttgt tacctgtgat aaaatggtat ccttagctgg gctgcgcagc
    18601 gctttcccca atcgctcagc aaccattaaa agcgcagcca cctgccaaag ccccgtaagt
    18661 gccagaagcg gtactgaaat aagcaaacca taaccaatga aggtaaaaag ccagtatgcc
    18721 ttagtcttat cacttaagta gcctgaaaca agccgtatgg cataaccaat gaactccccc
    18781 aaaccagcaa caacaccaac ccacagcgca tttgcaccca attcttttaa atactgcgca
    18841 ttgacgcttc gtgcaccttc atagatgatg tcgccaagta agcttatgat tccaaaaagg
    18901 attataaact ggtacgctcg ttttttaatg tcagtcatca gttttcctcc atatcacgta
    18961 ttgcaaaaat ccagcgcagg taacgcttta tatcaacaag gctcatatta taaccaagat
    19021 aaattccgtc aacccacaat tcaaagtgaa gatgctgtcc ccatttttga ccctgtgctt
    19081 cgccaagcaa tcctgagttg cctacccatc ctatacgctg ccctttcttt acccgcacac
    19141 ctgtattgat cttcccatca atttttgaaa gatggttgta ggtagatagt atcccattac
    19201 catgatcaat ccatacctgt ctgccgccaa atgaacgctt gatgaatgtt gcgggatttt
    19261 tttgatagta ttgcgatagg gacttccatt cctcctcggt aggtggcaca tagtcaatat
    19321 cagcacgcac taccacacca tcagcacagg acaacactgg cgtagtttca tctacaggga
    19381 ttggcagaaa attatcttct ttgaaataat aaaagatatc aagtccataa tgagtaccac
    19441 cacggtaccc tcgaggtgca ttgggatagc ctgaatcatt ttcagggaac aacccatttt
    19501 ttatggggaa acagagatta tacaatcgtt tatcataata ttgtaatttg gcgcttcctg
    19561 catatacagc aatctctgct attgctgcat aaccgtcaat ggttgcatca tgtggtatta
    19621 atattctgaa tgtactggca tcaataccct ttacatttac tttaacagtc ctttgatatg
    19681 tactatctcc aaaaaaacct tttttatctt tctgtataaa attaacccgt gaataatcaa
    19741 accatttacc atcatacatg aattgcagga tgcagtatgt gatggtgttg taattatctt
    19801 ttgttcctgc agtaattact atatcagtta ttatacgttt tacaccaaaa tctatttcaa
    19861 tccactgatc atcaccttta gctgaaatcc atgaggtagt ttcatcatca tctagtgctt
    19921 tttcaccttt atggttttta ctgaaattgc tgcttgtggc atagtatatt ttttgctggg
    19981 tattgtttcc acatatgata tattcttttc cctgcgtgct ccgctgagga acaaaatttg
    20041 aaattagcaa caacgatagt aacgaagcaa tggttttagc cataatttat attttcctac
    20101 aaaatttttt atatttctcc ctactaccac tgggacatgg agcattacgt cctattttat
    20161 tgattaccac aatcatttaa aaacaacttt tgaaacttta aaattcttca tctatttaga
    20221 tatggttatc ggcaactttt gtttatatgt atcatctcct tcttgttttt gcaagcaatc
    20281 gtaatatctc aagatataac catatcaaag
//
LOCUS       DAORYL010000055        19936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_2288_length_19936_cov_11.999598, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000055 DAORYL010000000
VERSION     DAORYL010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 19936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19936
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2288_length_19936_cov_11.999598"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..231
                     /locus_tag="PLV81_10365"
     CDS             91..231
                     /locus_tag="PLV81_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10540.1"
                     /translation="MKPNTYCIDTNCSYWRNVENLYSELVAKNENLRNKIFSFRSFIA
                     AE"
     gene            complement(333..809)
                     /locus_tag="PLV81_10370"
     CDS             complement(333..809)
                     /locus_tag="PLV81_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10541.1"
                     /translation="MKQKPLNFQQAIIDFMKSKANRMEKELNVPGSWYFNEGDEQEIK
                     SWTDEEAAKVWEKIKHNIFKLGCSGLRYELCPFCHHYGYEHNGCYKALKNPICIKCGY
                     GKRHGICIGEEGHVSQYKQILQSFEDSRISMYKFFTNEYYTELINKIEKENIKAIA"
     gene            complement(1006..1554)
                     /locus_tag="PLV81_10375"
     CDS             complement(1006..1554)
                     /locus_tag="PLV81_10375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation endopeptidase HyCI"
                     /protein_id="HOM10542.1"
                     /translation="MHGLLNASKIFKKHVTIVIGKITTCGGDSYCSMDKQCKKLILGI
                     GNHLRSDDAAGSIVARKLKLLGFTSIDAEFMPENYIGVIRKHKPEKIIIVDTCTMALP
                     PGSVRTIPLHKIHGGIVTTHSMPLSAVIQQLREITPKIMLIGIQPETIEVGDTVSEAV
                     TNAINQIVTTISNGEEVNLPML"
     gene            1595..2794
                     /locus_tag="PLV81_10380"
     CDS             1595..2794
                     /locus_tag="PLV81_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOM10543.1"
                     /translation="MITKGLIEKLFQAASIQRWNDHARPTEFAEIDKQAHKMIIAYTI
                     AKFQEEENPGCVNWIHLIEGGIFELLHRVIVTDIKPPIFHKIMKEKGKELNEWVFAQL
                     DDDISPVKGNFKETFLRYFQDSHFAPFEKKILHAAHYLATNWEFQIIYQSNKFLYDIE
                     KTKQEIESQIEYHIDLTGVQKILLHRNIAGFINLCGQLRFQQRWAQTPRIPKTSVLGH
                     MLIVAILSYFCSRELGACAKRMYNNFFASLFHDLPEVLTRDIVSPVKRSIKGLEELIK
                     EYEIIEANNRIFPLIPEKWHSEMQYFMFDEFENKIFQNDSVIKGVSAEDLNTKYNEDR
                     FNPLDGQMLKACDDFAAFLEASFSIKYGIKPEALESAKRNIYQKYRQKHLTIGDMDFI
                     MLFDYFN"
     gene            2998..3393
                     /locus_tag="PLV81_10385"
     CDS             2998..3393
                     /locus_tag="PLV81_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HOM10544.1"
                     /translation="MKNKFKNIFFNFRDTVGALLVAIKHPDVSLWSKIIIAVAIAYAL
                     SPVDLLPDFIPFIGYLDDAVIVPFLFFIAIKSIPQSVYEECKVQAHTAFKKSPGFIVL
                     GIVIVILLWAFIGFFMYALVSGILRLLKL"
     gene            3390..4097
                     /locus_tag="PLV81_10390"
     CDS             3390..4097
                     /locus_tag="PLV81_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10545.1"
                     /translation="MIAIINPDTPQAIITGLMHHGFSPVTIPLCPYVERPVAGHPDIQ
                     LCIIGNNIIYEPRIEKTFLQVLSQSTYTLIPGKSHLQATYPYDCAYNCAYTGSIAFHN
                     TKVTDSSIKQILTPYSVPLIHVNQGYTRCSTCIVDSHAIITSDTSIHNAAVNNGIDSL
                     LIQPGYIELPGYKYGFIGGASCMCSDTMYFTGRLSHHPDYSNIVNFIAIHNKKIVFLS
                     DLPAIDTGSIFFIDMNR"
     gene            4156..4701
                     /locus_tag="PLV81_10395"
     CDS             4156..4701
                     /locus_tag="PLV81_10395"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOM10546.1"
                     /translation="MNIPDMTTCLSLLQQYGTLPNVLEHSLQVHKVARAIVDHLNGSV
                     TINRDLVCAAALLHDITKSRALKTGEKHDISGKELLESLGYPEIADIVGQHVFLRDTS
                     PTSPLNEADIVYYADKRVMHNEIVTVDQRIEDLLKRYGTTQERCNKILENKDIILIIE
                     KKLTRHLVKPLEEIIASLKHE"
     gene            4694..>5256
                     /locus_tag="PLV81_10400"
     CDS             4694..>5256
                     /locus_tag="PLV81_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOM10547.1"
                     /translation="MNKHYCILDCYVDEPACFGVPPFVSPYPRYVYGALIDAGIYEDE
                     IVYLTIDSLRASEYRLPHNFDYVFLIGGAIVPGKYLGFKIGTVTEITKIIATNKRQKF
                     AIGGMIARLVKDFPNTTLVTGDIEQFAYSVATGNDPVTDNYRTYKDLARWAFYGSKLI
                     TLHPQFPNLICEIETYRGCPRRNHCSF"
     assembly_gap    5257..5356
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5357..6199
                     /locus_tag="PLV81_10405"
     CDS             <5357..6199
                     /locus_tag="PLV81_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOM10548.1"
                     /translation="IDNANPGTIYYFPEESQTIIDCIAHTVTPGDTMPLGVESFDPAV
                     IQKNNLKLHKDQVLQVVQLINDAGGKTINGIHRLLPGINLIHGLIGETADTFKINYES
                     LVEILDKGLLLKRINIRSLFPFPGTIAAQKQTHNIMIENRYRFYRDKIRNEIDHAMIT
                     RLFPIGTVIAHMQVLSCVHGYSYAKEIRSYAITAKIPVVLPLFSFVDCIVVGHQERSL
                     LCLPYPLAINSLPQKALEAIPGVGKKGASTIIVHRPFANMNDVKQYLEHVPEIIMKAI
                     AKGF"
     gene            complement(6196..6519)
                     /locus_tag="PLV81_10410"
     CDS             complement(6196..6519)
                     /locus_tag="PLV81_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10549.1"
                     /translation="MDVLVSFLMKLLAIAIYIAMGALVSWIFYNLKRRDLFGGFIGGM
                     VIGVIGALIGGFILDKLLLDITIKILRFLVYDTGVNIIAAFIGGYAAVYVMNKLNHDK
                     ERKRY"
     gene            complement(6526..7143)
                     /locus_tag="PLV81_10415"
     CDS             complement(6526..7143)
                     /locus_tag="PLV81_10415"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOM10550.1"
                     /translation="MIVLINEINPQKRFIKKAKEILEEGGIIIFPTDTVYAYGCDIND
                     KRAIERLYHIKRIPKNKPLSFIFSDISEISQYVRNVSDQAFKIMKKAFPGPYTFIFQA
                     SKLVPKIAITNQKTIGVRIPDNNIALELVQALGHPIMTASVSTKEGDYVIDPVDLDKE
                     YRNAVDMILSCGPKASDPSTVIDFSGGDIKIVRKGKGELFFIEEE"
     gene            7720..9759
                     /locus_tag="PLV81_10420"
     CDS             7720..9759
                     /locus_tag="PLV81_10420"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOM10551.1"
                     /translation="MSPIDLKIKSRDIDTLISVIKENNPEIHIENIRKAYEIANEAHK
                     DQQRLSGEPYIVHPMEVAIILANLKMDETTIIAALLHDVVEDTPINLDKVRELFGEEV
                     ATLVDGVTKISSLKKHTKQKEQAETLRKMLLATIQDARVIIIKLADKLHNMRTIMFQP
                     EYKQKYIAQEVLDIYAPLAGRLGMSKIRSELEDLAFRVLYPDDYNAIVEHIKARRSEL
                     EAFIDSIRNVLQQRLKELNIEAQIQGRIKHHYSIFKKLQQGRTFDDIFDLRAIRIITN
                     EVKDCYGILGIVHTIWTPIPSRFKDYIAVPKSNMYQSLHTTVIGPDGHPLEIQIRTWD
                     MHATAEMGIAAHWLYKEGIKSFKPKYKNITILNNINEWQNEIKDTREFIKELKMDLYE
                     DEIFVFTPKGKIIKLAKGSTPIDFAYLIHTEIGNHCTGAKVNNQLVPLKTELKSGDIV
                     EILTSKKGHPSPAWLKFVKSSNARYKIRNWLRKNVELEDEHTEKEEKDKEKEKKEKIA
                     SVSIPEKELVKLKSYTNKKKVGITIDGTNNVMIRLSQCCQPIPGDDVVGFITRGRGIT
                     VHKKNCPALQRLQNESERFVDIQWEQSESTYPVKIAVEAMDRTNLLKDVADEISLTKT
                     NIIKAEASVVAKDQALLKFVLEVKSLNHLNDIMKRLKQIKNVTRVYKLNEKVILK"
     gene            9766..10359
                     /locus_tag="PLV81_10425"
     CDS             9766..10359
                     /locus_tag="PLV81_10425"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOM10552.1"
                     /translation="MNIILGSSSPRRKYLLQQMFNTITIIHPDVEEIPHPHEKPLQFV
                     QRASSDKLNNIRTKIFTNPSLVITCDTIVTIDNEILGKPADFDHAYYMLSTLSGRTHH
                     VISSIRLLYNNKNDIRDHNDYEMTQVTFKKLNDGDITAYLSQIDYKDKAGAYAVQEHG
                     SMIIAKIDGSLTNVIGFPLRCFFRMIADMDIIQKLFQ"
     gene            10449..10982
                     /locus_tag="PLV81_10430"
     CDS             10449..10982
                     /locus_tag="PLV81_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10553.1"
                     /translation="MRQRKQYIINKKFQLKTTFSVIAIVFVIVAIIIAAIGVNAAYNN
                     KKLINIIQIQDNIVEALIAYSQSPHDSDQKLAIQNIANDHVKNINTIKEIIELNNFLL
                     LIIITFVILQGIILYFVLIRKTHKIAGPIYVMSNYFNDIIKGNIPNPRPLRKNDELQD
                     FYALFMKMVDAIRSRQE"
     gene            complement(11069..11779)
                     /locus_tag="PLV81_10435"
     CDS             complement(11069..11779)
                     /locus_tag="PLV81_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10554.1"
                     /translation="MKHKNINVTLCIMVLILCIYGTYLYSQQMFKSNEFIQTDISPDR
                     LQFMPVTEDFRNYFVLQCIGNTVSVLIGDFTGTEKLISLAKDTNGDGKADEVYEYFPE
                     IKKLTTPFKPTTAFYKNIDELKKDIISGDIFTVNYAYKMYSLPLLKAKLKTGRDVYRF
                     KHGYSVKMYDPDAPTTIAGEFFFGKNEGKYDLIFTTYYYKLYRTKISPPLSYSVYCKD
                     TTDKYIAEIVEELLKMVE"
     gene            11954..13075
                     /locus_tag="PLV81_10440"
     CDS             11954..13075
                     /locus_tag="PLV81_10440"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOM10555.1"
                     /translation="MRSSLFYIIFTAILIIYLTANFYVLQRIQKLVPNQYKILTATFI
                     SILALSFLLGRILERYTVCAASDFLIWIGALWLGIFIYLFFGFIIGDSIQGIVHIFIK
                     TLNIQKAAYSIVIIVSIIITSVGFINARTPHVKEIAIHIDKPSSPTHLKIAYASDIHL
                     GSIIANSRLQNLVRLINSAQPDLIIFGGDILDEDIKPVIENNLGELLIQLKARYGVIA
                     VPGNHEYIGGIQNAINYIKDHNIRVLRDEVYSIGDIQIIGRDDRSKYAFTGSRRLPLE
                     NLIKQIDISKPVIVIDHQPFKLSQTASYPVDFLLSGHSHHGQLFPFNYITKKLYEISW
                     GYKVINKTNVYVSSGYGTWGPPIRTNSYPEIVIFNVYFK"
     gene            13433..15286
                     /locus_tag="PLV81_10445"
     CDS             13433..15286
                     /locus_tag="PLV81_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HOM10556.1"
                     /translation="MNALWIVTAAATFYMLAYRFYGAFLAAKVLALDETRVTPAYRLN
                     DNTNYYPTNKWVLFGHHFAAIAGAGPLIGPVLAAQFGYLPGFLWILIGSVVAGGVHDM
                     VILVASIRKDGKSLAEIAKEEIGPVTGLAALFAVLFILIVAIAGLGLVVVNSLYNNPW
                     GTFIIAMTIPIALLMGVYMKSIRPEKVLETSIIGIVLLLITVIYGRHILGTNIEGFFT
                     LNQHNLAITLAVYGFIASVLPVWLLLAPRDYLSSFMKIGVIVLLAIGVVAVHPDLHLP
                     ALTQFIHGNGPIIPGKLFPFVFITIACGAISGFHSLISSGTTPKMIENEKYALPIGYG
                     AMLTEGFVSMMALIAAAVLIPGDYLAINTHLSFDTLAQMGFPVDKINDLSKLVGIDLA
                     GRPGGAVSLAVGMAYIFSSIPFLSHLMAYLYQFAIMFEALFILTTIDAGTRVARYVVQ
                     ELGGYVYKPFGELRSLSGNIIASLLVVIAWGYFIYTGSVQTIWRMFGTANQLLAMLAL
                     CIGTTVIIKMNKIKYIWVTLIPMLFMLSTTFTAGITLIIEYIPKALSSQPDAYSFMIN
                     IFLLTLMILLALFILLDSVYKWYGFITGKRPIKNNTEQPSQYSKEFPKMSD"
     assembly_gap    15459..15558
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15559..16107
                     /locus_tag="PLV81_10450"
     CDS             <15559..16107
                     /locus_tag="PLV81_10450"
                     /inference="COORDINATES: protein motif:HMM:NF027524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOM10557.1"
                     /translation="NLIIQLMPGLPCDSFEKSIETARKTVLLYPDGVRIYPTVVIKDT
                     QLETLYKENKFIPLSLDEAIAVCAFMYDIFTSNNIPVIRMGLHPFDDAVADTIIAGPY
                     HPSFGYLVKSRYLRNKLENNIKNFLQNNGNIQTLTIKLPQNKSAEYYGPHKENIAFLR
                     NVFHTITLQFYSADVQDLEIYG"
     gene            16380..18254
                     /locus_tag="PLV81_10455"
     CDS             16380..18254
                     /locus_tag="PLV81_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOM10558.1"
                     /translation="MQHHQKVLFIDLSTGYYRLARYPIGDFFGPVDLGLHIAQRYYTT
                     NIGVGVLAGSIFPGSNRLFVNGYSPCWNSFFISSMGGAGLVFDNLGINMIALSKKAPT
                     PSVLYLNRIHGEEIEIEIKPIDVFRIWESGHGGVYSLMHEVLSMYAGKYLTDPRVIAT
                     GPASHATDFGALCSAPVKDGTLTWVDTWAGRGGFGSKLFREHGIAAIIYGGTYIDEDF
                     RDRKVADEWFIDKYSKKLAAKDLEATTKYRFDPQFNTGGTFGVNFATIGGKLLAFNYR
                     SIYLTEEERLAIHQKLVVDHYLKQFNEETIATKSMKTCGEPCTAVCKKMRDIYKKDYE
                     PYQAMGPLCGVFDQRSAEMLVHKADMYGFDAISVGGVLSWLMECLDRKLLKPEEAGVG
                     HLPVFSHVNFSVETDSKHNAEIASQLLDAIIHKKGILDFTEGARKFARKLAREKSKDI
                     IDCFVYNANARKGWIVPNQYWVPGVLSPMPIMGKYYMYYGYDFLPPRELGRKNAHRMI
                     KELMLDNTGFCRFHRLWAEDMIPEIMNSLYGMHKEFLTNLDMTASRINSRNVSIYWES
                     TRNIDFIKMYLKRQHEVEKVNDKELVHWISQFEQNPQEAAYNYWFEILKGVHESLREF
                     "
     gene            18270..19160
                     /gene="hslO"
                     /locus_tag="PLV81_10460"
     CDS             18270..19160
                     /gene="hslO"
                     /locus_tag="PLV81_10460"
                     /inference="COORDINATES: protein motif:HMM:NF001033.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOM10559.1"
                     /translation="MEDNLSLRYICEEYNLRIYAALSKNVLQEMITIHNTTPHATIAF
                     GRTLNAALLLASSSIKPHSNDTLSINISCSGPLKEIVVQVDGKGNVRGFVGNPTVDVE
                     LQSDTLNISKALGAGVITVTRDLGLREPYKSVSPIIYGDIARDIAYYLLESEQIPSAL
                     LLAVNCNKEGAITRSGGILIQTFPSTPRQVIDVIESKLQSGFSLDKKLEEEQSIKSII
                     EELIGKEIKELGSLPIQHRCRCTKELLATILESVETQELEDMIQKDHGAHITCVFCRK
                     EYVFTEEELQKIVVKKEKVH"
     gene            19613..19789
                     /locus_tag="PLV81_10465"
     CDS             19613..19789
                     /locus_tag="PLV81_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10560.1"
                     /translation="MNDDLERQWKIILDFVGNIISFEEAIDELVKLGYTQKAAQEYMI
                     MVRSDFIKAQNNLM"
BASE COUNT         6376 a         3741 c         3481 g         6138 t
ORIGIN      
        1 ttaatttttt acttgccttt ttagcatttt agtatatatt taaattacta agtttaatta
       61 tttatcctta attatttaag gaggattatc atgaaaccaa atacctactg tatagatact
      121 aattgttcat actggagaaa tgttgaaaat ctttattcag aattagtagc aaagaatgaa
      181 aatttaagaa ataagatttt cagtttcaga agttttattg cagctgaata accgtatata
      241 agcaacccat aagaacctcc gatgtaaaac taaaaggggc tggcagcaaa ccagcccctt
      301 ttaattatag tacattgtat ggaagcctca gattatgcta ttgccttaat attttctttt
      361 tctattttat ttataagttc agtataatat tcgttggtaa agaacttata catgctgatc
      421 cgtgaatctt caaatgactg caatatctgc ttatactgac ttacatggcc ttcttcgcca
      481 atacagatac cgtgcctttt gccatatcca cactttatac agatgggatt ttttaaggct
      541 ttataacatc cattatgctc atatccataa tgatggcaga atgggcataa ttcatagcgc
      601 aaacccgaac aacctaattt gaaaatattg tgtttaatct tttcccatac ttttgctgct
      661 tcttcatcgg tccaggattt gatttcctgt tcgtcgccct cattaaaata ccagctaccc
      721 ggcacattta actctttttc catacgattg gctttagact tcataaaatc aatgattgct
      781 tgctggaaat tgagtggttt ttgtttcata acaatatccc ttttattgta ttctgcaaat
      841 tatatatccc acccttttat atatatctac ctgatgtata gcaaataata accaaaatac
      901 ctcaaaaaga atatattaat ttgcagtatg tacttctatt taataaagta taaccattcg
      961 gtattttttc aaggtttttt ttataaaatt taaaatattt ttatgctaaa gcataggtaa
     1021 attgacctct tccccattac taattgtcgt aacaatctga tttatagcat tagtaactgc
     1081 ctcacttaca gtatctccca cttctatggt ttcaggctgt attcctatta acatgatttt
     1141 gggggttatt tcccgtaatt gctgtataac ggctgataac ggcatactgt gggtcgttac
     1201 aataccgcca tggattttat gcagtggtat tgtacgcaca gaacccggag gcaaagccat
     1261 agtacaggta tcaacaataa tgattttctc aggtttgtgt ttccgtataa caccaatgta
     1321 attttcgggc ataaactctg catctatact tgtaaacccc aatagtttta gcttgcgggc
     1381 aacgatactt cctgcagcat catcactgcg cagatgattt cctattccca gtatgagttt
     1441 tttgcactgt ttatccattg aacagtaact atcgccccca catgttgtta ttttaccgat
     1501 aactatcgtt acatgctttt tgaatatttt acttgcattt agaagtccat gcaagtttta
     1561 ttttcatatg cgatagttac tggagaaata ctctatgatt acaaaagggt tgattgagaa
     1621 gttgtttcag gcagcaagca ttcagcgatg gaatgaccat gcacgcccca cagaatttgc
     1681 cgaaattgat aagcaggcac ataagatgat tattgcctat acaatagcaa aatttcagga
     1741 agaagaaaat cctggttgtg taaactggat acaccttatt gaaggtggca tctttgaact
     1801 tttgcaccgg gtgatagtaa ctgacattaa accgcccatt tttcataaga taatgaaaga
     1861 aaaaggcaaa gaactcaacg aatgggtttt tgctcaactt gatgatgata taagtcctgt
     1921 taaaggaaat tttaaggaaa cattcttgcg gtattttcag gactcgcact ttgcaccatt
     1981 tgaaaaaaag atattgcatg cagcgcatta cctggctacc aactgggaat ttcagatcat
     2041 ttaccagagt aataaatttt tatacgatat tgagaaaaca aaacaagaga tagaaagcca
     2101 gatagaatat catattgatt taaccggtgt acagaagatc cttttacatc gcaatattgc
     2161 cgggtttata aatttgtgcg gacagctacg gtttcagcaa cggtgggcac aaacgccacg
     2221 cataccaaaa acatcggtac tggggcacat gctcattgta gcaatacttt catacttttg
     2281 ctcacgggaa ttaggtgcat gtgcaaaacg catgtataat aatttttttg cttctctttt
     2341 tcatgacctg ccagaagtgc tgacccgcga cattgtttca cctgttaaac gctcaattaa
     2401 aggtctggaa gaactcatca aagaatatga aattattgaa gccaataatc gtattttccc
     2461 actcatacct gaaaagtggc acagcgaaat gcaatacttt atgtttgatg aatttgaaaa
     2521 taagatattt caaaatgatt ctgtaattaa gggagtatct gctgaagatt tgaatactaa
     2581 atacaatgag gacaggttca atccgctgga tgggcagatg ctcaaagcct gtgatgattt
     2641 tgctgccttt cttgaagctt cattttccat aaaatacggc ataaaacctg aagcgctgga
     2701 atcagcaaaa cgcaatatct accaaaagta caggcaaaag cacctgacca ttggtgatat
     2761 ggattttatt atgctgtttg attattttaa ttagttgtta gttacattaa tccttacagg
     2821 cttacgctgt ttaaaaatta ttgtttaaca aatgtattag cagccacact gatgattttg
     2881 ccaaaaatag caataaaatt gtatcaaaac gtcttctgta tatataatat aatattttaa
     2941 aaacttattc aataactttt attgacatcg tttacagtgt tttaccatat acaacgtatg
     3001 aaaaacaaat ttaaaaacat ttttttcaat ttcagagata ccgttggggc attgctggtt
     3061 gctatcaagc atcctgacgt ttcactatgg tcaaaaatca tcattgcagt ggcaatagcg
     3121 tatgctctgt cccccgtgga tctactacct gattttattc cattcattgg atatctggat
     3181 gacgctgtta tagttccttt tttatttttt atagccataa aatctatccc acaatcagtg
     3241 tatgaagaat gcaaggttca ggcgcatact gcatttaaaa agtcaccggg atttattgtg
     3301 ctggggattg tgattgttat tcttttgtgg gcattcatag gtttttttat gtatgcttta
     3361 gtttcaggaa tactaaggct tttgaagcta tgattgctat tatcaatcct gacacgcctc
     3421 aggctattat aacaggatta atgcatcatg gattttcacc tgtaacaatt ccactgtgtc
     3481 cttacgttga acggcctgtt gcagggcacc ccgatataca gctgtgcatc ataggcaata
     3541 atattattta tgaaccgcgt attgaaaaga catttttaca ggtattatcg caatcaacat
     3601 atacactgat accaggcaaa agccacctgc aggcaacata cccttacgac tgtgcatata
     3661 attgcgctta tactggcagc atagcgtttc ataatacaaa agtaaccgat agttccataa
     3721 aacaaatctt aactccatac tccgtccctc tgattcatgt aaatcaggga tacaccagat
     3781 gctctacctg tattgttgac tcacatgcca ttattacatc agatacttcc attcacaatg
     3841 ctgctgtaaa caatggcatt gattcactgc tcatacaacc aggatatatt gaactgccag
     3901 gctacaaata cggttttatt ggtggggcaa gttgtatgtg ctctgacacc atgtacttca
     3961 cagggcgttt aagccatcac cctgattaca gtaatatagt caactttatt gcaatccaca
     4021 acaagaagat tgtcttttta tccgatcttc ctgcaattga tactggctca atctttttta
     4081 ttgacatgaa tagataacat gctattcata tggtgtgtac ataacattaa tcataaaaca
     4141 ccatggatac cattcatgaa tattcctgac atgacaacat gtttatcact actgcaacaa
     4201 tatggaactt tgcccaatgt gctggaacat tcattgcagg tacacaaagt tgcccgtgcc
     4261 attgttgatc atctcaacgg cagtgttaca atcaatcgtg atttagtatg tgctgcagcc
     4321 ctacttcatg acatcaccaa atcccgtgca ctgaaaacag gtgaaaagca tgacatttca
     4381 ggcaaagagc ttttagaatc gttaggatat ccagaaatag ccgatattgt agggcagcat
     4441 gtattcttac gcgacacttc acccacctca ccgctcaatg aagcagatat agtatattat
     4501 gcagacaaac gcgttatgca taatgaaatc gttaccgttg accagcgcat tgaagattta
     4561 ctcaaacgct atggaaccac acaagaacgc tgtaacaaaa ttcttgaaaa caaagatatt
     4621 atactgatca ttgaaaaaaa attaaccaga caccttgtaa aaccactgga agagattatc
     4681 gcatcattaa agcatgaata agcactactg catccttgat tgttacgttg acgaaccggc
     4741 gtgttttggt gtaccaccgt ttgtttcgcc ctacccgcgc tatgtgtatg gtgcactcat
     4801 cgatgccggc atctacgaag atgaaattgt gtatcttacc attgattccc ttcgtgcgtc
     4861 tgaatacagg ttgccacata attttgatta tgttttttta attggggggg cgatagttcc
     4921 tggcaaatac cttggcttta aaataggtac tgtcactgaa ataacaaaaa tcattgcaac
     4981 aaacaaacga cagaaatttg ccattggcgg gatgatagct cgcctggtta aggattttcc
     5041 caatacaaca ctggtaaccg gtgatattga acagtttgca tattccgttg ctactggcaa
     5101 tgatcctgtt accgataact atcgcacata caaagacctg gcacgctggg cgttttatgg
     5161 cagtaaactg atcacacttc atccacaatt ccccaacctt atctgtgaga tagaaaccta
     5221 tagaggatgc ccacgccgca atcactgctc attttgnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnattg acaatgcaaa tcctggtacc atctattact ttcctgaaga
     5401 atcgcaaact attattgatt gcatagctca tactgtaaca ccgggcgata caatgccgtt
     5461 aggtgttgaa tcatttgatc cagcggttat tcaaaagaac aaccttaaat tgcacaaaga
     5521 tcaggttcta caggttgtac aattaataaa tgatgcaggt ggaaaaacta ttaatgggat
     5581 tcacaggctt ttgcctggta ttaatcttat tcatggactt attggtgaaa ctgctgacac
     5641 atttaaaatc aactatgaat cactggtgga aattcttgat aaaggtttgc ttttaaaaag
     5701 gatcaatata cgcagtttgt tccccttccc gggaactatc gccgcacaaa aacaaacaca
     5761 caatataatg attgaaaacc gttaccgttt ttaccgcgat aaaattagaa acgaaattga
     5821 ccatgctatg attacaagac tattccccat tggtactgtc attgctcata tgcaggtgct
     5881 gtcatgtgta catgggtatt cgtacgcaaa agaaattcgc agttacgcca taacggcaaa
     5941 aattcctgta gtattgccgc tttttagctt tgttgattgt atcgttgttg gtcatcagga
     6001 acgttcactg ttgtgccttc cctacccatt agcaattaac tcactgccac aaaaagctct
     6061 ggaagccata ccgggtgttg ggaagaaagg tgcatcaaca attattgtgc atcgcccttt
     6121 tgcaaatatg aatgacgtaa aacaatattt agaacatgtt cctgaaatta ttatgaaggc
     6181 aattgcgaaa ggattttaat agcgtttgcg ctccttatca tgattaagct tgttcatgac
     6241 atacactgct gcatatccgc ctataaacgc tgcaataata ttaacaccgg tatcgtatac
     6301 taaaaatcgc agtattttta ttgttatatc cagtaacagc ttatcaagaa tgaatccgcc
     6361 tatcaatgca ccaataacac ctataaccat accacctata aatccaccaa acagatcacg
     6421 gcgtttcaaa ttgtaaaata tccacgacac cagcgcaccc atggctatat aaatagcaat
     6481 ggcaagtaat ttcattaaaa agcttactaa gacatccatg gttgcctact cctcctcaat
     6541 aaaaaaaagt tcacctttac ctttgcgtac aatctttata tcaccaccag aaaagtcaat
     6601 aacagttgat ggatcacttg cttttggtcc acaacttaat atcatgtcaa ccgcatttcg
     6661 gtactctttg tctaaatcaa ccggatcaat gacataatcc ccttcttttg tgctgacact
     6721 ggctgtcatg attggatggc ccagggcctg caccagttca agcgcaatat tgttatcagg
     6781 gattcttaca ccaattgtct tttgattggt tatagcaatc tttggtacca actttgatgc
     6841 ctgaaaaata aatgtatagg gacccggaaa tgcttttttc ataattttaa aagcctgatc
     6901 ggaaacattg cgtacatact gagaaatttc cgaaatgtct gaaaatatga aactgagtgg
     6961 cttatttttt ggtattcgct ttatatgata caatcgttca attgcgcgtt tatcattgat
     7021 atcacaccca tacgcataca cagtatcagt tgggaaaatg ataatacccc cttcttcaag
     7081 aatctctttt gctttcttga taaaacgctt ctggggattt atttcattaa ttaatactat
     7141 catagctatt attatacatt aattaacgga aattttcttt atttaattta gtgtattgat
     7201 tgtgggaagc atttaaaata taaggctttt gcaggagtgc ctgctgcaaa agccttcaac
     7261 ccggtaaatg tgcgaatagg agtgtcttgt aattatcaac cattataatc atcggaattt
     7321 tttttaaaac ttaaagtatt tttatgtaaa tatttagatg ttattgacta aaaacttcat
     7381 gtttaccaat cgtttcttta aaatcttata aaggcgcctt catccgctaa atgatcataa
     7441 tttttacaca accattaata aaagagattg cttcgtcgtc cctaagggac tcctcgcaat
     7501 gacgagaaga gcgacattgc gtatttttca cttgtccttg cgagcaaagc gtggcaatct
     7561 cattacacag acaaagattc ccatttgatt gaagatagac acatttacaa ataaaatagt
     7621 gattgaaaaa atagttttta aattattata tttgtattat attattgtat atgtactata
     7681 ctataacaat taacaaatta tttatgatat cataaaacca tgtcgcctat agatttaaaa
     7741 ataaaaagca gggatataga tacactgatt tcagttatta aggaaaataa tcctgaaata
     7801 catatcgaaa acataagaaa agcatacgaa atagccaacg aagctcataa agatcagcag
     7861 cgtttatctg gtgaacctta tattgtgcat cccatggaag tagccattat tctggctaac
     7921 cttaaaatgg atgaaaccac cattattgct gcgttgctcc atgatgttgt tgaagatacc
     7981 ccaatcaact tagacaaagt aagagaactg tttggcgaag aagttgcaac acttgttgac
     8041 ggcgtaacaa aaatatcgtc tttaaaaaaa catacaaaac aaaaggaaca ggctgaaacc
     8101 cttcgtaaaa tgttactggc aactattcag gacgctcggg tcataattat taaattagct
     8161 gataaactgc acaatatgcg tactatcatg ttccagcctg aatataaaca aaaatatatt
     8221 gcacaggagg ttcttgatat ttatgcaccc ctggcagggc gccttggtat gtccaaaatt
     8281 cgcagcgaac tggaagatct tgcatttaga gtattgtatc cagatgatta taatgctatt
     8341 gttgaacaca taaaggcacg tagaagcgaa ctggaagcat ttattgattc tatacgtaat
     8401 gtattgcagc aacgattgaa agaattaaac attgaagctc agattcaggg aaggattaaa
     8461 catcattatt caatttttaa aaagcttcaa cagggaagaa catttgatga tatttttgac
     8521 ctcagggcta tcagaattat taccaatgaa gtaaaggatt gctatggaat tttaggtatt
     8581 gttcatacga tatggactcc cattccttcc cgttttaagg actatattgc tgtaccaaaa
     8641 tcaaacatgt atcaatcatt acatacaact gtgattggtc cagatggtca tccgctggaa
     8701 attcagatac gaacatggga tatgcatgcc accgcagaaa tggggattgc tgcacactgg
     8761 ttatacaaag agggtattaa atcattcaag cctaaataca aaaatattac aattctcaat
     8821 aatataaatg aatggcagaa tgaaatcaaa gatacccgtg aatttattaa agaactcaaa
     8881 atggacctgt atgaagatga aatttttgtc ttcactccta aaggcaaaat tataaaattg
     8941 gccaaagggt ccactcccat agattttgca tacctgatac atactgaaat tggcaatcac
     9001 tgcactggtg caaaagtcaa taatcagctt gtcccactta aaactgaatt gaaaagtgga
     9061 gatattgttg agatattaac cagcaaaaag ggtcatccat caccagcatg gcttaaattt
     9121 gtcaaatcat caaatgcccg atacaaaata cgtaactggt tacgaaagaa tgtagaactt
     9181 gaagatgaac atactgaaaa agaagaaaag gataaggaaa aagaaaagaa agaaaaaatt
     9241 gccagcgtca gtatacccga aaaagaactt gtaaaactta aaagctatac caacaaaaag
     9301 aaagttggca tcaccataga cggtactaat aatgtaatga tacgcctttc acaatgctgc
     9361 cagccaattc caggagatga tgttgtggga tttattacca gaggccgggg gattactgtc
     9421 cataaaaaaa actgcccggc attacaacgg ttgcaaaatg aaagcgaacg ctttgtagat
     9481 attcaatggg aacaatcaga aagcacctat cctgtcaaaa ttgctgtcga agccatggat
     9541 agaactaatt tattaaagga tgttgctgac gaaatatcct taacaaaaac aaacattatc
     9601 aaagccgaag cttcagtagt tgctaaagat caggcattgc ttaaatttgt tcttgaagta
     9661 aaaagcctta accacctaaa tgatattatg aaacgactga aacagataaa aaatgtaacc
     9721 agagtataca agcttaatga aaaagtaatc cttaaataga aaaatatgaa tataatactt
     9781 ggttcatcat ctccgcgaag aaagtatctt cttcaacaaa tgttcaatac tataaccatc
     9841 attcatcctg atgtagaaga gataccacac ccccatgaaa aaccactgca atttgtacaa
     9901 cgcgcttcca gcgacaagct taacaatata cgcacaaaaa tttttactaa cccttcactc
     9961 gtcataacct gcgacaccat agtaactatc gataatgaaa ttttaggaaa acctgcggat
    10021 tttgaccatg catattatat gctctcaacg ttatcaggaa gaacacatca tgtcatatca
    10081 tcaatacgtt tactgtataa caataaaaac gatattcgcg atcataatga ttatgaaatg
    10141 actcaggtta cttttaaaaa actcaacgat ggcgatatta ccgcatattt atcacagata
    10201 gattataagg ataaagctgg tgcgtatgca gttcaggaac atggcagcat gattatagca
    10261 aaaatagacg gttccttgac caatgtcatt ggtttcccat tgcgttgctt tttcaggatg
    10321 atagctgata tggatataat ccaaaaactt tttcaataat ttaaaatttt aagattgaca
    10381 tataaaaaaa agagattatt atttatatat tcatcgaatt ataaaatctc aaaaaaaggt
    10441 actatactat gaggcagaga aaacaataca tcataaacaa aaaatttcaa cttaaaacca
    10501 cattttctgt cattgctatt gtttttgtga ttgttgcaat tattatagct gctatcggtg
    10561 taaatgcagc atataataat aaaaagctta taaacattat tcaaatccaa gataacattg
    10621 ttgaagcatt gattgcatat tcacaatctc cacatgattc agaccaaaaa ttagcaatac
    10681 aaaatattgc caatgaccat gtcaaaaata tcaataccat aaaagaaatc attgaattga
    10741 ataacttttt gttgctgatt atcattactt ttgttatact gcaaggaata atactttatt
    10801 ttgtattgat ccgcaaaaca cataaaattg caggacctat atatgttatg tcaaactact
    10861 tcaatgatat tattaaaggt aatatcccaa atccacgtcc tttacgcaaa aatgatgagc
    10921 tacaggattt ttatgcatta tttatgaaaa tggttgatgc catacgctca aggcaggaat
    10981 aaaaaataat ataaggtaca tgatatataa taaaaaaacc tgctttgcat agcaggtttt
    11041 tttatataca atgacttaaa taaaatattt actccaccat ctttaaaagc tcttcaacta
    11101 tttctgctat atatttatct gttgtatcct tacagtatac tgaataactt aatggcggtg
    11161 atattttagt tctgtaaagc ttataatagt acgttgtaaa aatcaggtca tatttccctt
    11221 catttttccc aaagaaaaac tcgccagcaa ttgtagttgg cgcatcagga tcatacattt
    11281 ttactgaata accatgttta aaacgatata catccctacc tgttttaagt ttagccttga
    11341 gtaatggcaa agaatacatt ttatatgcat aatttactgt aaaaatatca cctgatataa
    11401 tatctttttt taactcatca atatttttgt aaaatgctgt tgttggctta aaaggagttg
    11461 ttagcttttt tatttcaggg aaatattcat atacttcatc agcctttcca tcaccattgg
    11521 tatcttttgc aaggcttata agtttttcag taccggtaaa atcgcctatc aacactgata
    11581 ctgtattgcc tatacactgc aacacaaaat aatttctgaa atcttcggta acaggcataa
    11641 attgcaacct gtctggagat atatctgttt gaataaattc attactttta aacatttgct
    11701 gggaataaag atatgtgcca taaatgcaca ggatcagcac cataatacat aaagtaacat
    11761 ttatattttt gtgtttcata ataataaccc caatgttatg atttacatac cctttcacta
    11821 tttattgtat ttcaaataaa tagtgaagtc aataacaaaa aaatatttta ttactatttt
    11881 aggcttttaa ttttatacta aatatataac ctgtgctaat attagcatat accattatta
    11941 cggaattaac tttatgcgaa gttcactgtt ttatatcatc ttcaccgcaa ttctgataat
    12001 ctatcttaca gccaattttt acgtgctgca acgtatacaa aagctggtgc ccaatcagta
    12061 taaaattttg acagcaacgt ttataagcat ccttgctctt tcgtttctgc ttggccgaat
    12121 acttgaacgc tatacagtgt gtgcagccag tgacttcctc atctggattg gagcactctg
    12181 gttgggtatt ttcatatatt tattttttgg gtttattatt ggcgatagta tccagggaat
    12241 agttcatatc tttataaaaa cacttaatat tcaaaaggca gcctacagta tagtcataat
    12301 cgtaagtata ataatcacat ctgtgggatt tattaacgcg cgcacccctc acgtaaagga
    12361 aatagccatt catattgata agccttcatc accaacgcat ctgaaaattg cgtatgccag
    12421 cgatatccat ctgggatcaa tcatagcaaa ctctcgtttg caaaacctgg taaggcttat
    12481 taatagcgca caaccagatc tcatcatttt tggtggtgat atactggatg aagatatcaa
    12541 acccgtaatt gaaaataatt taggcgagtt actgatacaa cttaaagcca gatacggcgt
    12601 aattgcagta cccggaaacc atgaatatat tggggggata cagaatgcta tcaattatat
    12661 caaagatcat aatatacggg tgttacggga tgaagtatat tccattggcg atatccagat
    12721 aatcggacgc gatgacagaa gcaaatatgc atttaccggt tccaggcgtt taccattaga
    12781 aaatttaata aaacagatag atatttcaaa accagttatt gtcattgacc atcagccatt
    12841 taaactatca caaacagcat catatcctgt agactttctt ctttccggcc actcacatca
    12901 tggtcaatta tttcccttta actatatcac aaaaaagctt tacgaaataa gctggggata
    12961 caaagtcata aacaaaacga acgtatatgt atcaagtggt tacggtacat gggggcctcc
    13021 catacgtacc aatagctacc ctgagatagt tatatttaat gtatatttta aataaatatt
    13081 taccactcta tcactgcggt aagcctaatt tcattttgct tatagtattt attcattaaa
    13141 attttttaaa gaaggaatac atgaaaactc ccaacctatt atagtaagga tgaatactgg
    13201 attactttgc tattaaaagc atgaccacca attattagtg caatacatat ttttttacta
    13261 aaatctcttg acacaacgtg agaacaggtg tctaccgtta gcacataaaa ttttcttcat
    13321 caggagatat actatgaaga agcttttaaa aattgccttc tggattgtaa tatcattatt
    13381 atttgcacta tcctttgcta tagtcacaca gatttacaac cctcaggaaa aaattaacgc
    13441 attatggata gttactgcag ccgccacatt ctacatgctg gcataccgct tttatggtgc
    13501 ttttttagct gcaaaggtgc tggcacttga tgaaacacgt gttactccag catatagact
    13561 gaacgataat accaattact atcccaccaa taaatgggta ctatttggtc atcattttgc
    13621 tgctatagca ggtgcaggcc cattaattgg ccctgttctt gccgcgcagt ttggctatct
    13681 cccgggtttt ttgtggatcc ttataggctc agtagttgct ggtggcgtgc atgacatggt
    13741 gatacttgtt gcatcaatcc gcaaagatgg caaatcgctg gcagagattg ctaaagagga
    13801 aattgggcca gtgacagggc ttgcggcttt atttgcagta ttgtttattt taatagttgc
    13861 tatcgcagga cttggccttg tggtagtaaa ttctttatat aataatccat ggggcacttt
    13921 catcatagca atgaccatcc ccattgcact gcttatgggt gtatacatga aatccatacg
    13981 ccccgaaaag gttcttgaaa catcaattat tgggattgtg ttattactga taaccgttat
    14041 ttatggcagg catattcttg gaacaaacat tgaaggattc tttactttaa atcagcataa
    14101 tctggcgata actcttgctg tctatggctt tatagcaagc gtattgccag tatggctcct
    14161 ccttgccccc cgtgattacc tgtcatcatt tatgaaaatt ggcgttatag tgctgcttgc
    14221 tattggcgtg gttgctgtcc atccggattt acatttaccg gcattgacac aatttattca
    14281 cggcaatggt ccaatcattc cgggaaaact attcccattt gtatttataa cgatagcttg
    14341 tggtgctatt tcaggattcc actcactcat ctcatccggt acaacaccca agatgattga
    14401 aaacgaaaag tatgcgttac ccattggcta cggtgccatg cttactgaag ggtttgtatc
    14461 aatgatggct ctcattgctg ctgcagtgct tattcccggt gattaccttg caatcaacac
    14521 tcacctgtca tttgatacac tggcacagat gggattcccg gtggacaaaa tcaatgacct
    14581 gtcaaaactt gttggcattg atttagctgg ccgccctggt ggtgctgttt cacttgctgt
    14641 gggtatggcg tatatctttt catccatacc ttttttaagc catttgatgg catacctgta
    14701 tcagtttgcc ataatgtttg aggcgctatt tattttaacc acaattgatg ctggtacccg
    14761 agttgcacgg tatgtagtcc aggaactggg tggctatgtt tacaaaccat ttggcgaatt
    14821 acgatcttta tcaggtaata ttattgccag tttgcttgtt gttattgcat ggggttattt
    14881 tatctacacg ggttcggtac aaacaatctg gcgaatgttt ggcacagcaa atcagctttt
    14941 ggccatgtta gctctgtgta ttggcacaac ggttatcatc aaaatgaata aaattaagta
    15001 catctgggtt acactgatac ccatgctttt tatgctcagc accacgttca ctgcgggtat
    15061 aacccttata attgaatata taccaaaagc actttcatca cagcctgatg catacagttt
    15121 tatgattaat atttttttat taaccttaat gattctgtta gctttgttta ttttactgga
    15181 ttctgtgtat aaatggtatg gatttattac cggcaaacga cccatcaaaa acaatacaga
    15241 acagccatca cagtattcaa aagaatttcc aaaaatgagt gattaaatga ttacatgccc
    15301 aaaacacatg taaccatacc tttctttata ccacattctg gttgcaggca ccgatgtgtg
    15361 ttctgcaacc agatatacac tagcaataca cgatttcttc ccaatgaaga atatattgtt
    15421 gaaaaaattg agcagtattt acaaaccata aaaccaggnn nnnnnnnnnn nnnnnnnnnn
    15481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15541 nnnnnnnnnn nnnnnnnnaa ccttataatt cagcttatgc ctgggcttcc atgcgattct
    15601 tttgaaaaat caattgaaac agcacgcaaa actgttttac tataccctga cggtgtacgc
    15661 atctatccca ctgttgttat aaaagatact caattagaaa cattatacaa agaaaacaaa
    15721 tttattcctt tgtccttaga tgaagctatt gcagtatgtg ctttcatgta cgacatattt
    15781 accagcaaca atattccagt aatccgaatg ggtttgcatc cttttgatga tgcagtagct
    15841 gataccatca ttgcgggtcc atatcatcca tcatttggtt atttagtaaa atcgcgatat
    15901 ttacgcaata agcttgaaaa taacattaaa aactttttac agaataacgg caatatacag
    15961 accctcacca taaaattacc ccagaacaaa agcgcagaat attatggccc acataaagaa
    16021 aatattgctt ttttacgtaa tgtttttcac actattacat tgcaatttta ttctgctgat
    16081 gtacaggatc tcgaaatata tgggtaaatt cagatagtgg tgctgaatca caacgtaatt
    16141 caggatttag cattacttaa tggtttagtc attttgaacc tgaatcagaa tctctctcat
    16201 caagatgatg aatcaagttc agtatgacgt agtatgcaaa aacccctgaa tcaagttcag
    16261 gaagactgtt tgtgcttcag gatgaccgtt tgtgcgttag gatgattgtt tatgaatcag
    16321 gatgactgac catgaggtaa aaggcctgga tttaacaaaa taattttcag gagaaaaata
    16381 tgcaacatca tcaaaaagta ctcttcatag acctttccac cggttactat cgcttagcac
    16441 gataccctat tggcgatttt tttggtcctg ttgatcttgg gctgcacatt gcacagcgct
    16501 actacaccac aaatattggc gttggtgtat tagctggatc tatcttcccc ggctctaata
    16561 gactctttgt caatggatac tccccctgct ggaatagctt tttcatttca tcaatgggtg
    16621 gcgcaggcct ggtgtttgat aacctgggta taaacatgat tgccctttcc aaaaaagcac
    16681 caacaccttc ggtactgtat cttaaccgta ttcatggtga agaaattgaa atagaaatta
    16741 aacccattga tgtattcaga atatgggaaa gcggacacgg cggtgtgtat tccctgatgc
    16801 atgaagtact ttccatgtat gctggcaaat atctaactga tccccgtgtc atagccactg
    16861 gccctgcttc acatgcaaca gattttggtg cgctgtgttc ggcacctgtt aaggatggca
    16921 cgctcacctg ggttgatacc tgggctggac gtggtggatt tggctcaaag cttttccgcg
    16981 aacatgggat tgccgcaata atatacggtg gtacatatat tgacgaagac ttcagagacc
    17041 gcaaggtcgc tgatgaatgg tttattgata aatacagcaa aaagctggca gctaaagact
    17101 tagaagctac aaccaaatat cgctttgacc cgcagtttaa cactggtggt acctttggtg
    17161 ttaattttgc tactatcggc ggtaagctcc ttgcatttaa ctatcgctca atttatttga
    17221 ccgaagaaga aaggcttgct attcatcaaa agcttgttgt tgatcattac ctgaagcaat
    17281 ttaatgaaga aactatcgcc accaaaagca tgaaaacctg cggtgagcct tgtactgccg
    17341 tgtgtaaaaa gatgcgtgac atctacaaaa aagattacga accgtaccag gcaatgggac
    17401 cactgtgcgg cgtgtttgat cagaggtcag ctgagatgct tgtacacaaa gctgatatgt
    17461 atgggtttga tgctatttca gttggtggcg tactatcatg gcttatggaa tgccttgatc
    17521 gtaaactttt aaaacctgaa gaagcgggtg ttgggcacct tcctgttttt tcgcatgtaa
    17581 attttagtgt agaaacggat tcaaaacaca atgctgaaat tgcaagtcag cttcttgatg
    17641 caatcattca taaaaaagga atacttgatt ttaccgaagg ggcgcgcaaa tttgcccgta
    17701 aattagcacg tgaaaaaagt aaggatatca ttgattgctt tgtgtataac gccaacgcgc
    17761 gcaagggatg gattgttccc aaccagtact gggttcctgg tgtactatcg cccatgccta
    17821 ttatgggtaa gtattatatg tactacggct atgatttttt gccgccgcgt gagttaggcc
    17881 gcaaaaacgc tcatcgtatg attaaagaac ttatgcttga caacactggc ttttgccgtt
    17941 ttcaccggct gtgggccgag gacatgatcc ctgaaatcat gaattcactg tatggaatgc
    18001 ataaagaatt tttaacaaat cttgacatga ccgcaagcag aatcaacagc cgcaatgtgt
    18061 ccatttactg ggaatcaacg cgcaacattg attttataaa aatgtacttg aagcgacaac
    18121 acgaagttga aaaagtcaat gacaaagaat tagtacactg gatttcacaa tttgaacaaa
    18181 atccacagga agctgcatac aactactggt ttgaaatttt aaaaggtgtt catgaatcat
    18241 tacgtgaatt ttaaggggcg atagttacca tggaagataa tctaagttta cgatatattt
    18301 gcgaggaata caatctcagg atctatgctg cactgagtaa aaatgtattg caggagatga
    18361 tcaccattca taacactacg ccacatgcaa ccatagcatt tggtcgcacg ctgaacgctg
    18421 ccctgcttct tgcttcgtct tcaattaaac cgcattcaaa cgacacgttg agcattaata
    18481 ttagctgcag tggcccttta aaagaaatag tggtacaggt tgatggcaaa ggaaatgtac
    18541 ggggatttgt tggtaatccc actgttgatg tggaactgca atctgataca ctgaatatat
    18601 caaaagcatt gggcgcaggt gttattactg taacacgtga tttagggtta cgtgagccgt
    18661 ataaaagtgt ttcgcccatc atttatggtg acattgccag ggatattgct tactacttac
    18721 tggagtcaga gcaaatacct tcagcactgt tacttgcggt caattgcaat aaagaaggag
    18781 ctattacccg ttcaggggga attttaatac aaacatttcc cagcacacca cgtcaggtta
    18841 tagatgtaat tgaaagcaag cttcaaagcg gcttttcgct tgataaaaag cttgaagaag
    18901 aacaatcaat caagtccatc attgaagagc ttattggcaa agaaattaaa gagcttggct
    18961 cattacccat acagcacagg tgcagatgca caaaagaact attagcaaca atactggaat
    19021 cagttgaaac gcaggaactg gaagatatga tacaaaagga tcatggagca catatcacct
    19081 gcgttttttg cagaaaagaa tatgtgttca ccgaagaaga attgcagaag attgtggtga
    19141 agaaggaaaa ggtgcattaa ctcaatgtca ttgccagctt ctgctaccgt gatacacgat
    19201 gcaacactta ccctgtcatt ccgagggagc caccaatagg gacgaccgaa gaatccttgc
    19261 cgttttcaaa taagttattg tgagtgctaa catattcggg acgaaataat atcattaatg
    19321 gtatgctgat attttacaag caataaagcc aaaaacccaa aaatgtttaa ttatcttata
    19381 ttcataaatc aaaaaagcta aaccaaacgc ctatcattgt ataacacgga tttattgtat
    19441 tgtttgaact aatgcaattt cttttcaaac tttgcataac tttaactctt tgttatcatt
    19501 aacataaaca tataaactgt aaaattttaa tcagtatcat tttcttgact gttatatgaa
    19561 taagctatat attattcttg aatcattact atgtatcaat tatggatttt ttatgaatga
    19621 tgatctagaa aggcagtgga aaattatttt agattttgtt ggtaatatta tctcttttga
    19681 agaagcaatt gatgaattag taaagcttgg ctatacacaa aaagctgcac aagaatatat
    19741 gataatggtt agaagcgatt ttataaaagc acaaaataat ttgatgtaag ttccacaatt
    19801 ttattttaat gtctcattaa atcccactat tggtagctta aacccaaata caaatcaaca
    19861 gtcccatcat aattttttac tctttaaatc ccactattgg tagcttaaac acatcctggg
    19921 cgggtacaac cgctgg
//
LOCUS       DAORYL010000056        19722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_2317_length_19722_cov_12.238623, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000056 DAORYL010000000
VERSION     DAORYL010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 19722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19722
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2317_length_19722_cov_12.238623"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="PLV81_10470"
     CDS             <1..618
                     /locus_tag="PLV81_10470"
                     /inference="COORDINATES: protein motif:HMM:NF022059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOM10561.1"
                     /translation="MGTAQSKGTKLLSVSGDCQNPGIYEVEFGTTVNTLLEMVGGRTA
                     KAVQVGGPSGTCISKKDFGRRICFDDLATGGSIIIIGPDRDLFEIVHNFMDFFVEESC
                     GWCVPCRAGNVILKQKLEKIMSGKASTKDVQELEDWCKIVKATSRCGLGQTSPNPIYT
                     TIQNFREMYEAKVKKDTDYVSTFDLLAAVEESCKAAGRIPKVEEH"
     gene            620..1315
                     /locus_tag="PLV81_10475"
     CDS             620..1315
                     /locus_tag="PLV81_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011394105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOM10562.1"
                     /translation="MSTINFTIDGKACKAKPGQTIVEAAKDNGIYIPTLCHFEGLKPA
                     GTCRICTVKVGGRNMAACTTPVTEGMVVENKTADLEDYRKAIIEMLFVEGNHMCPTCE
                     KSGNCELQALGYRYMMMAPRFPFLFPRRTLDASLPKIVIERNRCVQCLRCVRGITTAD
                     GKRIFGVVNRGGHVEITVDKDLAATMTDEMAKKAMDLCPVGAILKKEVGFAIPIGKRK
                     FDSKPIGSEIEKA"
     gene            1344..1883
                     /locus_tag="PLV81_10480"
     CDS             1344..1883
                     /locus_tag="PLV81_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HOM10563.1"
                     /translation="MSKPVVATASLAGCFGCHMSILDIDDRILKLIELVEFNKSPIDD
                     IKTFTKQCDIGLIEGGCCNSENVENLKLFRKNCKILISVGECAIMGGLPAMRNGIPVK
                     ECLEEAYLKCPTVDDNIIPNDDELPMILDRVYPCHEIVKIDYFLPGCPPRADLIWEAL
                     VALLTGKELELPYEVVKFD"
     gene            1908..3356
                     /locus_tag="PLV81_10485"
     CDS             1908..3356
                     /locus_tag="PLV81_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOM10564.1"
                     /translation="MARKITIEPVTRVEGHGKVTIQLDPQNNVLDSRFHIVEFRGFER
                     FVQGRPYWEAPVLVQRLCGICPVSHHLAAAKAMDVIVGVGPDGLTPTAEKMRRLMHYG
                     QMFQSHALHFFHLVSPDLLFGVDADPAIRNVIGVAMKFPDLAVQGVMMRKFGQEIIRV
                     TAGKKIHGTGAIPGGINKNLSVQERDEFLKGADPMNIKKMIEWAQAAVNLFKDYHKKN
                     KDFIDSFAAFPSNHLSLVRKDGALDLYHGVLRAVDADGKKILNDVDYQDYLKYVGEEV
                     RSWSYMKFPYLKSLGKKDGWYRVGPLARLNTADFIPTPLAQKEFEEFKAYTNGKPNNM
                     SMHMHWARLIELLHSAEMIEQLLNDPDLQGDKLVAKGTKVKEGVGLIEAPRGTLFHHY
                     RINDNDQIEMANLIVSTTNNNEPMNRAINWVAVNQISGKKEITEGMLNAVEVAIRAYD
                     PCLSCATHAIGKMPLEVTLVDAEGNVVDQKRK"
     gene            3359..3841
                     /locus_tag="PLV81_10490"
     CDS             3359..3841
                     /locus_tag="PLV81_10490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOM10565.1"
                     /translation="MLNNVKCLVYGYGNPGRQDDGLGVELALRIENDEYLSKYVDVDY
                     NYQLNIEDALTISEYDLVIFADAALNIDNPYELTKIQPAHTITFTTHELSAESILALC
                     QDLYNKKPESYILAIRGYEWEMGLPMTSKAKCNLDDAYTFLTEFIENHFSRCIAGKGI
                     "
     gene            3869..4243
                     /locus_tag="PLV81_10495"
     CDS             3869..4243
                     /locus_tag="PLV81_10495"
                     /inference="COORDINATES: protein motif:HMM:NF018228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM10566.1"
                     /translation="MANKKIILVIDDDPDILDSIKAILSANGFDVVTAMSGKEGIDAV
                     ASSKPDLILCDMMMERIDAGTKVAQEIKKSNPSIPIYLLSSIGTATATNIEIDKLGFN
                     GVFQKPVNPDNLVATIKKALTM"
     gene            complement(4349..5533)
                     /locus_tag="PLV81_10500"
     CDS             complement(4349..5533)
                     /locus_tag="PLV81_10500"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOM10567.1"
                     /translation="MAIKYLDQINVSNKKVLIRADFNVPYDKNMNITDDTRITSTLPT
                     IEYCLKQNATVILVSHLGRPKGKPVPEMSLKPVAKRLSELLKKDVVFIDKPITQGIQD
                     EINKLKPGDVALLENIRFYPEEEKNDENFGKLLGALADVYVNDAFATAHRGHASNEAI
                     TRYVKECVAGFLMKDELEYFKKALVQPQKPVTAIIGGVKISTKIDVLKNIIPKVDNLL
                     IGGGMSYTFLKALGYNIGKSVVEDDQLHTAKEIIELAKKHNVNLLLPEDIVVANKFDS
                     PEGKEVVPITSIPDNKEGVDIGPATRKKFAEIIKQSKTVIWNGPLGAFETKEFAEGTG
                     EIAKVVAESGCTSVIGGGDTGAAINSSGYADKMTYISTAGGAFLELMEGKILPAVKAL
                     DK"
     gene            complement(5627..6625)
                     /gene="tatC"
                     /locus_tag="PLV81_10505"
     CDS             complement(5627..6625)
                     /gene="tatC"
                     /locus_tag="PLV81_10505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOM10568.1"
                     /translation="MAKEQNPKNSKKKKSTAKPKTKKEITASKSKSRTKQSAIQEAQA
                     QAAHTKKEQKKPEKNLIEALIKEADENAHNDSLEEVEVDPDALRRGDKPMNIVDHLDE
                     FRSRLLIVLVTVIVLTVLGFYFSDFLLDAINKPFIDSGFKLNIFNLVEGFVLRIKASF
                     VMGLLIGLPLLVYHLWQYIKPAIDIDNRKFAVYSIIAAVILFYAGLGATYFYLLPLSI
                     KVLLSFTPPDMENMINASKYFNFAILFALAIGITFEMPIIIMILTRIGIVSPSFLISK
                     RKYAIVLIWIFAAVITPTTDPLTLTLVAVPLMLLYELSIVISKFIVIRKKRKELTQ"
     assembly_gap    6717..6816
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6817..9639
                     /locus_tag="PLV81_10510"
     CDS             <6817..9639
                     /locus_tag="PLV81_10510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOM10569.1"
                     /translation="VLNNKVQENRILTDTFELYIRRINTNRILLQIKNKQIQQLKYSD
                     LTRENEDNIKFSSAPFFSAFDLLYDLIHITDKNGLIVFANKAIKKLFGDIRGQYCYKV
                     FACEDDYCPDCKLSLVLDGNTIRYEVKLNKNNQYYDVINSPFVDFDGNVYKISIMRNL
                     EEIVEKQNTLVLFQNAINKSKNLVCFANENGEILYANDTFIKYFGANYREENNILRLM
                     NYSKDDVYQSSDNKEIKITLSDNEIYFKVFIYPIQSLSNETIFVFIASDITDLKKLEK
                     KIAIEKNYFQSIIDNTIDGFFVIDKNQKIIDCNPSFLKMFHITPDSFGTITLAELFDP
                     LYKREFEKKINYLKKSHISQITEVSSSNNGFKNYIISLNYLSTSESDDEQYYGFVKDI
                     TDITRLHKIIENERNYNRRIIETVKLGFVVINEFGEIIDYNNAFLHLIGRSGKNVKGE
                     MFSSYIKPVNKQNMDELTSSSPDMLRDFEAIIITADNQEKNVIVSMSKLYDILGNYLG
                     NFAFIRDITHQKKIENEIVQQSHQIIDLVNSLTKFTASVVRIDDMDNLLLQIHSFITS
                     ILNPDYLEILVFSIDRYISHKYINNEHIVTNTEISYKTSFIIQKLTKSKTIMFITNPI
                     IELNDEDQLLFSNVMRFKNINFIPIFIGKDLKMIILVAFNKEVEFINNVIITILTSVS
                     NLIAIAFEKINSIAEQVKMKEALDRYEKLVAIGRIIAGVAHEINNPLSIMQLDIAELD
                     QKLDQGDYDQEIKDIIRSLLEEIQRISNIVKQLKDYAKPAALNDDIINLNEIFRTYPL
                     KILSKNIQKKGIPININIPKQKIYVKMPLTRLIQVIMNILTNAEDAVVEKGEGSIQID
                     ISTEQRGEKIFARLSVKDTGIGIHEENKHLLFEPFFTTKPKEGTGLGLSISYSIIKSY
                     DGEIEIESKKGVGTEVIILLPFYKKT"
     gene            9880..11694
                     /locus_tag="PLV81_10515"
     CDS             9880..11694
                     /locus_tag="PLV81_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOM10570.1"
                     /translation="MIQYSESSMPAIFQKQVKNLGDQGCVSYKKGGVYVDLSWNQMDQ
                     LIRNFAYYLLSLGIKKGAKIALFSPNRYEWFVADMAILSIGAVNVPIYATNSSEEAYY
                     IIDNSDSVLCIAGTDDHLERILKVKKRLPKVKQIIVFDEIKTKTPGVITLSQAIEKGK
                     TYKNKQEFEKRIKSIRKDDLATIIYTSGTTGNPKGVMLTHGNFVANVNQILYDFSDYV
                     TYKDVCLSFLPLSHSLERTAGYYLAIAGGAKIAYAEDFSTVPQNLAEIRPNFIVSVPR
                     LYEKIHAGILARAADVKGIKKALFNWSLYVAKKNTPYVCQDKKPKGILGFQVRLADKL
                     IWSKLKAALGFDRIRIAVSGGGPLAVSDAEFFLGMGIVILEGFGLTETTPVTNVNRPG
                     MIQPGSVGPALKDTIIKIADDGEILIKGPQVMKGYYKNPKATKEVFTKDGFFKTGDIG
                     VIDEKGRLYITGRIKDIIVTAGGKNISPQNIENSLKESKYIEQVAVIGDRRKYLSALV
                     IPAFEELGKWAKKQGIQFANNKELIENDKVNELIAQEIQKYTKQFSRVEQIRKFKLLE
                     AEWSQASGELTPTLKVKRRVIEQKYAKEIESMYPKDMD"
     gene            complement(11750..13201)
                     /gene="ahcY"
                     /locus_tag="PLV81_10520"
     CDS             complement(11750..13201)
                     /gene="ahcY"
                     /locus_tag="PLV81_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOM10571.1"
                     /translation="MIQPLRKDTTSIPEFDAKIPYKVKDISLAELGRKELQLAENEMP
                     GLMALRQKYGSQKPLKGMKISGSLHMTIQTAMLIETLHHLGADIRWASCNIFSTQDHA
                     AAAIAKAGTAAVFAWKGETLEEYWWCTEQALIWPDGSGPDLIVDDGGDATLFVHMGVK
                     AEKDPSILDQEYDSKDMQCLIDRLRLSVANNPKRWTTIASKIRGVSEETTTGVHRLYQ
                     LEKTGELLFPAINVNDSVTKSKFDNLYGCRESLADGIKRATGIMVAGKVVVVCGYGDV
                     GKGCAQSMRGFGARVIVTEIDPICALQAVMEGYEVKTLEDVVEIGDIFVTATGCCDVI
                     RGEHMERMKDEAIVCNIGHFDSEIEMSYLENNKKCKRINIKPQVDKWILESGRSIIVL
                     SEGRLVNLGNANGHPSFVMSTSFTNQTLAQIELATKKLEKKVYTLPKKLDEEVARLHL
                     GRLGVKLTRLTPKQAAYLGIEVDGPYKPDHYRY"
     gene            13313..13840
                     /locus_tag="PLV81_10525"
     CDS             13313..13840
                     /locus_tag="PLV81_10525"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOM10572.1"
                     /translation="MSKKNYRILIFVLMMTLISCVPSQWQYRNIPLKRKQIINTATQY
                     IGTPYRHGGATPSGFDCSGYVFYVYNKNGIQIPRSTAQQYTKGQKIPYKMAQPGDLLF
                     FRINMNTISHVGIYVGNDTFVHAPSSGKYVRYDSINNTYWKKRFCGAVSYFKALPVKP
                     LNNKGDSYREERFVW"
     gene            13928..14410
                     /gene="coaD"
                     /locus_tag="PLV81_10530"
     CDS             13928..14410
                     /gene="coaD"
                     /locus_tag="PLV81_10530"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOM10573.1"
                     /translation="MQIGIYPGSFDPLTCGHLDIIKRATQLFDKLIVAIARNSEKFPL
                     FAVEERLAMLQECCRDISKVEITSFDGLLAEYCKKNNINFIVRGLRAIVDFEYEYAIA
                     LMNKELAPSVETIFLMSKSEFSFVSSKMVKEVASYGGDISRLVPQFVSSKLKEKFQSQ
                     "
     gene            14476..16011
                     /gene="rny"
                     /locus_tag="PLV81_10535"
     CDS             14476..16011
                     /gene="rny"
                     /locus_tag="PLV81_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOM10574.1"
                     /translation="MNIITILLIVALPIAALLGYLFRAYIGKIKLNTAEAQSRKIIQD
                     AVREAEAKRKELLLEAKDQLLKERNQFEKEMRERRIELQNIERKILQKEEMIDNKIQQ
                     LEKQEKLIQIKEKENLEKEQELNKHLEKHKQELERISGLTREEAKQLLLKNLENEVRF
                     EAIKLINKVEEEARRTADKKAKEIVLAALQRSASDYTQESTITTVSLPSDDMKGRIIG
                     REGRNIRTLENLTGVDMIIDDTPEVVVISGFDPIRREIARLSLERLIENGRIHPARIE
                     EVVEKVKNELEENMLEEGERAAFELGIPGLSKDALYHVGKLKYRSSYGQNVLSHSKEV
                     ANLAAIMAGELKLDVATAKRAGLLHDIGKGSIVEGEGAHAIVGAELAKKFGENDVVVN
                     IIASHHNDKEPESFEAILVQVADAISASRPGARRESLDTYLKRLENLENIAYGFKGVE
                     KCYAIQAGREIRVMVSNEQVTDEEATVLARDIASKIESELKYPGIVRVTVIRETRIVD
                     YAR"
     gene            16078..16872
                     /locus_tag="PLV81_10540"
     CDS             16078..16872
                     /locus_tag="PLV81_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOM10575.1"
                     /translation="MNILLIGDIVGRPGRTIIKDHLSEICKEYSVHCVIANCENAAGG
                     MSITPEIAQELFSYGIHILTTGNHIFNNRTIIKLLESHSSILRPANFPSGVPGKGFTI
                     VRINNLNIAIVSLIGRINMEPVDCPFITFNTLYDEIRSKANIIIVDFHAEATSEKKAF
                     GWFADGRASAVCGTHTHVQTADETILPQGTAYITDVGMTGPFDSVIGMDKESSIHNFI
                     YHTRIKFKVAQNDPKLNAVLVVCNRQGKAVSITRIVKSYELSPSNS"
     gene            16953..18875
                     /gene="dxs"
                     /locus_tag="PLV81_10545"
     CDS             16953..18875
                     /gene="dxs"
                     /locus_tag="PLV81_10545"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOM10576.1"
                     /translation="MLSDIYGPQDIKRLSVKELAALADEVRQRILEVVSKNGGHLASS
                     LGVVELTIALHYVFDSPQDRIIWDVGHQCYAHKILTGRNQQFHTLRTFNGISGFPKVS
                     ESPHDHYNTGHSSTSLSLALGEAIARDVNNSKYNIVAVIGDGSMTGGMSFEALNHIGH
                     LKKDIIIILNDNEHSISKNVGALSEYLMRLITGGTYNKLRRKSYEFISRIPKIGPKLF
                     TLFDRIEARIKGIFIPSSFFEDLGIRYFGPIDGHNIPMMIEMFNRISKLNKGPKIIHV
                     YTKKGKGYVHAENNPAKFHGIGPFDIKTGKPVKKLSISYSEIAGRTLVDLAKHDKKIF
                     AITAAMKLGTGLAEFEEKFPSRFIDVGICEQHAITLASALAKNGLKPFVSIYSTFMQR
                     AVDQIIHDVALMNLPVKLLIDRAGIVGDDGETHHGLFDIGIIRSIPNFVLLSPTNGNE
                     LKDMINYAASYNNGPVAIRYPRGGDDKYEHSMPKGLLKIPQLKAYYKSQRPMIALFAA
                     GDMLSMAVEIHKKLHAYNIKSSVYSILSIKPLPVKQIEFIMNTIPYYIVLENSYANGG
                     IAEYINSQLDRSMRHKNLFNVAFPDSFITHGKVSELLRYYELDSDTITHKIISMINTE
                     KNSHAKKPLRSISSSK"
     gene            18835..19641
                     /locus_tag="PLV81_10550"
     CDS             18835..19641
                     /locus_tag="PLV81_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOM10577.1"
                     /translation="MPKNRLDQYLLQNNICTSREKAKNLIIAGYVTVNNQVIYKPSFI
                     VKENDVITVRELSQKFVSRGGEKLKKALDYFNIDVTGKIVLDLGSSTGGFVDCLLQYG
                     AEKVYAVDVGYGQLDYNLRINPKVIVMERKNARYLTIEDFDDHIHLITADLSFISIIK
                     VMQTMITIFDYEIDAILLIKPQFEAESFQHKKGVVKMPQYHKDILLKVFQQLQTMNIT
                     ILGLTYSPIKGPKGNIEFLLYGAINCNTRKSVDNYELLINSCVDEAHKVL"
BASE COUNT         6648 a         3135 c         3793 g         6046 t
ORIGIN      
        1 atgggtaccg cacagtcaaa gggaacaaaa ctgttaagtg tttctggaga ttgtcagaat
       61 cccggtatat acgaagttga atttggtaca acggtaaata cattgctgga aatggttggt
      121 ggcagaacag caaaggcagt tcaggttggc ggtccttccg gtacctgtat tagtaaaaag
      181 gattttggcc gacgaatttg ctttgatgat ctggcaactg gtggttcaat tattattatt
      241 ggcccggatc gagatctctt tgaaattgtc cataatttca tggatttctt tgtggaggaa
      301 tcctgtggat ggtgcgtacc ctgtagagca ggtaatgtta tcctgaagca gaaattagaa
      361 aagataatgt cggggaaagc atcaacaaaa gatgttcagg agcttgaaga ttggtgtaag
      421 atagtgaaag ccaccagtcg ctgcggtttg ggacagacat caccaaatcc aatatataca
      481 actattcaga attttagaga aatgtatgag gcaaaagtta agaaggatac tgattatgta
      541 tccacattcg atcttttagc tgctgttgaa gaatcatgca aagcagcagg aagaatccca
      601 aaggtagagg aacactaata tgagtactat aaattttact atagacggaa aagcatgtaa
      661 ggctaaaccc ggccagacaa ttgttgaagc agcaaaggat aatggcatat atattccaac
      721 attatgccat tttgaagggt tgaaaccagc tggtacctgc cgtatctgta ctgttaaagt
      781 tggaggcagg aatatggctg cctgtacaac acctgtaact gaaggtatgg tggtagaaaa
      841 caaaactgct gatctggaag attaccgcaa agctataatt gagatgctat ttgttgaagg
      901 caatcatatg tgcccaacat gcgaaaaaag cggtaattgc gagttacagg cgctgggata
      961 ccgttatatg atgatggcgc cacggtttcc gtttcttttc cccagacgaa cgcttgatgc
     1021 atcattacca aagattgtga ttgaacgaaa caggtgtgtg cagtgtttac gttgtgtgcg
     1081 gggtataacg acagctgatg gcaagcgtat ttttggcgtt gtcaataggg gtggccatgt
     1141 tgaaattacg gttgataaag atttagcagc aaccatgacc gatgaaatgg ccaagaaggc
     1201 aatggatttg tgcccggttg gtgctatatt gaagaaagaa gttggttttg ctatcccaat
     1261 tggcaagcgt aagtttgata gcaagcctat tggcagtgaa attgaaaaag cctgatttta
     1321 atgaagatct atggaggaat acaatgagta aaccagttgt cgcaactgct tcactggcag
     1381 gatgttttgg atgccatatg tccattctgg acattgatga tagaatatta aagttaattg
     1441 aattggttga atttaacaag tcaccaattg atgatattaa aacgttcacc aaacagtgtg
     1501 atattggatt aatagaaggt ggctgctgta acagtgaaaa tgttgaaaac cttaagttat
     1561 tccgcaaaaa ttgtaaaata cttatatcag ttggtgaatg cgctattatg ggtggattac
     1621 cagctatgcg caatggtatt cctgtaaaag aatgcctgga agaagcatac ttgaaatgcc
     1681 caactgttga tgataatatt attcccaatg atgatgaatt accaatgatt ctggacaggg
     1741 tatatccttg tcatgagata gttaaaatag attatttctt gccgggatgt cctccccgtg
     1801 cagatttaat ctgggaagca ctggtagcac ttcttaccgg taaagagttg gaattaccat
     1861 acgaagttgt aaagtttgac taattttgtt ttaggagaag aaagcttatg gctagaaaaa
     1921 taacgataga acctgtaaca agggttgaag gtcatggtaa agtgaccatt cagcttgatc
     1981 ctcagaacaa tgtactcgat tcacgttttc acattgttga gttcagagga tttgaacgat
     2041 ttgttcaggg cagaccatac tgggaagcac cggttctggt gcaacgcctt tgtggaattt
     2101 gccctgtcag ccatcatctg gcagcagcta aagcgatgga tgtcattgtt ggtgttggcc
     2161 cggatggatt aacacccact gcagaaaaaa tgcgaagact catgcattat gggcagatgt
     2221 ttcagtcaca tgcattgcat ttcttccatt tagtatcacc tgatttgttg tttggggttg
     2281 acgcagatcc agcaatacga aatgtgatag gtgttgctat gaaattccct gatttagctg
     2341 tacagggtgt tatgatgcgt aagtttggcc aggagataat cagggtaact gcaggtaaaa
     2401 agatacatgg taccggtgca attcctggtg gtatcaacaa gaacttaagt gttcaggaaa
     2461 gggatgagtt cctcaagggt gctgatccaa tgaacataaa gaagatgatt gaatgggcgc
     2521 aggctgcagt taatcttttt aaagattatc acaaaaagaa taaggatttt attgatagtt
     2581 tcgcagcatt tccatcaaat cacttaagcc ttgttcgtaa agatggtgct ctggatcttt
     2641 atcatggagt tttgagagcg gttgacgctg atggaaagaa gatattaaat gatgttgatt
     2701 atcaggatta tctgaagtat gtaggtgagg aagtcagatc atggagttac atgaagttcc
     2761 cataccttaa gtcgttaggc aagaaagatg gatggtatcg cgtagggcca ttggctcgac
     2821 tcaatactgc tgatttcatt ccaacaccgc tggcacagaa agaatttgaa gagttcaaag
     2881 catataccaa tggcaaaccc aataatatgt caatgcacat gcactgggca cggttaattg
     2941 aattattgca ctcagcagaa atgattgagc agctcttaaa tgaccctgat ctgcagggtg
     3001 ataagcttgt tgctaagggt acaaaggtta aagaaggcgt tggattaatt gaagcaccac
     3061 gaggtacctt attccatcac tatcgtataa atgacaatga ccagattgaa atggctaacc
     3121 ttattgtttc cactaccaat aataatgagc caatgaacag agcaattaac tgggtagcag
     3181 ttaatcagat cagtggcaaa aaggaaatta ccgaaggcat gcttaatgcg gttgaagttg
     3241 caatacgtgc atatgaccca tgtttaagct gtgcaacaca tgctattggg aagatgcctc
     3301 tggaagttac attagttgat gctgaaggca atgtagtaga tcagaaaagg aagtaagcat
     3361 gctgaataat gttaagtgtt tggtgtatgg atatggcaat cctggaagac aggatgatgg
     3421 tctgggtgtt gagctggcat taaggattga aaatgatgaa tatttaagca agtatgttga
     3481 tgtggattat aattatcagc tcaacattga agatgctttg accatttcag aatatgatct
     3541 tgttatcttt gctgatgcag cacttaacat tgataacccg tatgaactta caaagattca
     3601 gccggcacat acaattacgt tcacaacaca tgagctgtcg gctgaatcta tacttgcact
     3661 ttgccaggat ctttataata aaaaaccaga aagctatatc ctggctattc gtgggtatga
     3721 gtgggaaatg gggttgccta tgaccagtaa ggcaaagtgt aatttagatg atgcatatac
     3781 atttttaaca gaattcattg aaaaccactt ttctcgctgt atagcgggta aaggtattta
     3841 attttactac taaccaggag tgataagtat ggcaaataag aaaattatct tagtaattga
     3901 tgatgatccg gatattttag attctatcaa agctattctt tcagcaaatg gatttgatgt
     3961 agttactgca atgagtggaa aagaaggtat tgacgctgtt gcaagcagca aaccagatct
     4021 cattttatgt gatatgatga tggaaagaat agacgcaggt acaaaagttg cccaggaaat
     4081 taagaagagc aacccttcaa taccaatcta tcttttaagc agcattggta cagcaactgc
     4141 aactaatatt gaaatcgata agttgggttt taatggtgtt ttccagaaac ccgtcaatcc
     4201 agataatctg gtggcaacaa tcaagaaagc acttactatg tagtaactat cgcataacaa
     4261 attgtgcaaa aatttttatt gctatggtat taaaaaagcg ggttatcata aaacccgctt
     4321 ttttattttt atgtgcgata gttactgttt atttgtccag agctttaact gctggcagta
     4381 ttttcccttc cataagttcc agaaatgcgc cacctgctgt ggagatgtag gtcattttgt
     4441 cagcataacc tgaagaatta attgctgcac cagtatcacc gcccccgata acgcttgtac
     4501 aacctgactc agcaactact ttagcaattt ccccggtgcc ctcagcaaat tcttttgttt
     4561 caaaagcacc taacggccca ttccatataa ctgtttttga ttgttttata atttcagcaa
     4621 actttttacg ggtggcaggg ccaatgtcaa cgccctcttt gttatctggg atactggtaa
     4681 ttggaacaac ttctttacct tctggactgt caaacttgtt agccacaaca atgtcttctg
     4741 gcaatagtaa gttgacattg tgctttttgg ccagttcaat aatttccttt gctgtatgaa
     4801 gttggtcatc ttcaacaacc gatttgccta tattatatcc caatgccttt aaaaatgtat
     4861 aggacatccc cccgccaata agaaggttgt caactttggg aattatattt tttagcacat
     4921 caatttttgt tgaaattttc acaccgccaa tgattgcagt tacaggtttt tgtggctgaa
     4981 ccagagcctt tttaaaatat tcaagttcat ctttcattaa gaagccagca acacattctt
     5041 tgacatagcg tgttatagct tcatttgaag catgtccacg gtgagctgtt gcaaatgcat
     5101 cattaacata cacgtctgcc aatgcaccta ataatttgcc aaaattttca tcattttttt
     5161 cttcttccgg gtaaaacctt atattttcca gaagcgccac atcaccgggt tttaatttat
     5221 taatttcatc ctgaatcccc tgtgtaatag gtttatcaat aaatactaca tcttttttta
     5281 acaactctga aagccgtttg gcaacaggtt ttaatgacat ttcaggtact ggctttcctt
     5341 ttggccttcc taagtgagat actaaaatta ctgttgcatt ctgttttaaa caatattcaa
     5401 tagttggcaa tgttgaagtg atcctggtat cgtcagtaat attcatattt ttgtcataag
     5461 ggacattaaa atcagcccgt atcagcactt tcttattact gacattgatc tgatccagat
     5521 attttattgc cataattcac ctctctttta tgttttataa aaaatatacc atgaaacatg
     5581 catcaacgaa aacatagcgt cataaataag ctaaattgtc aaatagttat tgtgtaagtt
     5641 cttttcgctt ttttcgtata acaatgaatt ttgaaatgac tatcgatagc tcatataaaa
     5701 gcatcaaagg taccgctacg agcgttaaag ttagtggatc agttgtaggg gttatgacag
     5761 cagcaaaaat ccatatcagg actatcgcat attttctttt gctgattaaa aatgatggtg
     5821 aaactatacc aatgcgagtt agtatcatga ttataatggg catttcaaat gtgatgccaa
     5881 tggccaatgc aaaaagaata gcaaaattaa agtattttga agcattgatc atattttcca
     5941 tgtcaggagg ggtgaagcta aggagcactt taattgatag cggtaataaa taaaaatagg
     6001 tagctccaag gccagcgtaa aacaatatga cagctgcaat aatggaatat actgcaaatt
     6061 tgcggttatc aatatctatg gctggtttta tatattgcca aagatgataa acaagcagtg
     6121 gcaggccaat aagcagcccc atcacaaaag aggcctttat tcgcaacaca aaaccttcca
     6181 ccagattaaa tatatttaat ttaaaacctg aatctatgaa tggtttgtta atggcgtcca
     6241 gcaaaaaatc ggagaaataa aagcctaata cagttaacac tatcacagta accaacacaa
     6301 tgagcaatct ggagcggaat tcatccagat gatccactat attcattggc ttatcaccac
     6361 ggcgcaaagc atcagggtct acttccactt cctccagtga atcgttatgt gcattttcat
     6421 ctgcttcctt gattaaagct tctatcagat ttttttcggg tttcttctgt tctttttttg
     6481 tatgagcagc ttgtgcttgt gcttcctgta tagcagattg ctttgttctt gatttacttt
     6541 tgctggctgt aatttctttt ttggttttag gctttgctgt actttttttc ttcttcgaat
     6601 ttttaggatt ttgttctttt gccaatgtta taactccgta taataaatat tatgtgaata
     6661 ataaattaag ttattagaat tttgaataaa gtcatgacca atcaatttta aaaaaannnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngttt taaataataa agtacaggaa
     6841 aacagaatat tgacagatac gtttgaatta tatatcagac gtatcaatac gaatcgtata
     6901 ttattacaaa taaaaaataa acaaatacag caattaaaat attcagatct tacccgggaa
     6961 aacgaagaca atatcaagtt ttcttcagcc cccttttttt ctgcttttga ccttctatat
     7021 gatcttatcc acattactga taaaaacggt ttaatagtgt ttgctaacaa agcaattaag
     7081 aagttatttg gcgatatcag agggcaatat tgttataaag tctttgcttg tgaagatgat
     7141 tattgtcctg attgtaaatt aagtttagta ctggatggta atactattcg atatgaagta
     7201 aaattgaata aaaataatca gtattacgat gttataaact ccccatttgt tgattttgat
     7261 ggcaatgttt ataaaatttc tataatgagg aatcttgagg aaatagttga aaagcaaaat
     7321 acattggtat tatttcaaaa tgctataaac aaatcaaaaa atttagtttg ttttgcaaat
     7381 gaaaatggag aaatattata tgccaatgat acgtttataa aatattttgg tgcgaattat
     7441 agagaagaaa ataatatttt acgattaatg aattattcaa aagatgatgt atatcaatct
     7501 tcagacaata aagaaataaa gattacgcta agtgataatg aaatttattt taaagtattt
     7561 atttatccaa tacaatcatt aagtaatgaa acgatatttg tttttattgc aagtgatatt
     7621 actgatctaa aaaaattaga aaaaaagata gctatcgaaa aaaattattt ccagagtata
     7681 attgataaca ctatcgacgg tttttttgtt attgataaaa atcaaaaaat aatagattgt
     7741 aatccttcct tcctaaaaat gtttcatata accccggata gctttggtac aataacatta
     7801 gccgaactct ttgacccttt atataaaaga gaatttgaaa aaaagataaa ttatcttaaa
     7861 aagtcacaca tatcacaaat aaccgaggta agcagctcaa acaacggatt taaaaattat
     7921 ataataagtt tgaattactt atctacctct gaaagcgatg atgaacaata ttatggattt
     7981 gttaaagata ttacggatat tacccgcctc cataaaatta ttgagaatga acgaaattat
     8041 aacagaagaa taatagaaac tgttaaattg ggttttgtgg ttataaatga atttggcgaa
     8101 attattgatt ataataatgc ttttttacat ttaatagggc gttctggtaa aaatgttaaa
     8161 ggtgaaatgt ttagctcata tataaagcca gttaataaac aaaatatgga tgaactaaca
     8221 agttcttctc cggatatgtt acgggatttt gaagctatta taataactgc agataatcag
     8281 gaaaagaatg taatagtttc aatgtcaaaa ctatatgaca ttttaggtaa ttatttagga
     8341 aattttgcat ttatccgcga tattacgcat cagaaaaaaa ttgaaaatga aattgtacag
     8401 caatcccatc agattattga tttagtaaat tcattaacta agtttactgc aagtgttgtt
     8461 cgtatcgatg acatggataa tttattatta caaattcatt catttataac tagtatatta
     8521 aacccagatt atttagaaat tctagtattt tccatcgata ggtatataag tcataagtat
     8581 atcaataatg aacatatagt aactaataca gaaataagtt ataaaacttc ttttatcatt
     8641 cagaaattaa caaaaagtaa aactattatg ttcataacca acccaattat agaattaaac
     8701 gatgaagatc aattattgtt tagcaatgtt atgagattta aaaatataaa ttttatacca
     8761 atttttattg ggaaagatct taaaatgatt attctggtag ctttcaataa agaggtagaa
     8821 tttataaata atgtcattat tactatttta accagcgtat ctaatttaat tgcaattgca
     8881 tttgaaaaaa tcaattctat tgcggaacag gtgaaaatga aggaggctct tgacagatat
     8941 gagaaattgg ttgctatagg tagaataata gctggggttg ctcatgaaat taataatcct
     9001 ttatcaatta tgcaattgga tattgctgaa ttggatcaaa aacttgatca aggggattac
     9061 gatcaggaaa ttaaagatat tatcaggtct ttactggaag aaatacagcg tataagtaat
     9121 attgttaaac aacttaaaga ttatgccaaa cctgcagcat taaatgatga tataattaat
     9181 ctcaatgaga tattccggac gtatccatta aaaatacttt ctaaaaatat tcaaaaaaaa
     9241 ggtataccta tcaatatcaa tataccaaaa caaaaaatat atgtaaaaat gcctttaaca
     9301 cgattaattc aggtaattat gaatattctt acaaatgcag aagatgcggt tgtagaaaaa
     9361 ggtgagggta gcatacagat agatatatcc accgagcaga gaggagaaaa aatttttgct
     9421 cggttatctg taaaggatac tggaataggg attcatgaag aaaataaaca tttattattt
     9481 gaaccttttt tcaccacaaa acctaaagag ggtacagggt tgggattatc aatttcatat
     9541 tcaatcataa agagttacga tggcgaaata gaaatagaaa gcaagaaagg tgtaggaaca
     9601 gaggttatca tattattgcc attttataaa aaaacgtaat gcaataatta caaaagatag
     9661 aaattttcta tcattatata cttagaaaaa aattagcttc aatgcttact attattcaac
     9721 aataataata ttatacatcc ctgatactga aaaaatgatt taactataat aaaaatttat
     9781 agaatatatg ttgacataat aaatatagta ttcacatata ataaaattgt taaaaataca
     9841 tattattgta tataaaatat tatctatatg gaggatctta tgattcagta ttcagaatcg
     9901 tctatgcctg ctatatttca aaagcaggtc aaaaatttag gtgatcaggg atgtgtttcg
     9961 tacaagaaag gtggagtata tgttgatcta tcatggaatc aaatggatca acttatacgc
    10021 aattttgcat attatctcct ctcattgggt ataaagaagg gtgctaaaat tgcacttttt
    10081 tctcctaacc ggtatgaatg gtttgttgcg gatatggcta tcctgtcaat tggagcagtt
    10141 aatgttccca tttatgcaac aaattcatca gaagaagcat attatataat tgataattca
    10201 gatagtgtgc tgtgtattgc tggaactgat gaccatttag aaaggatttt aaaagtaaaa
    10261 aaaagattgc ccaaagtaaa acagattatt gtatttgatg agataaaaac taaaacacca
    10321 ggcgtaataa cactttcgca ggctattgaa aaagggaaaa catataaaaa taaacaggaa
    10381 tttgaaaaaa gaataaaatc tatcaggaaa gatgatctcg ctaccattat ttatacctcg
    10441 ggaactaccg gaaatcccaa aggggtcatg ctaacacatg gaaattttgt agctaatgtt
    10501 aatcaaatac tgtatgattt tagtgattat gtaacctata aagatgtatg tttatcattt
    10561 ttgcctttat cacattcact tgagagaact gcaggatatt atctggctat tgccggtggt
    10621 gcaaaaatag catatgctga agatttttca acagtacctc agaatttagc tgaaatcagg
    10681 ccaaatttca ttgttagtgt cccacgatta tatgaaaaaa ttcatgcagg cattttagcc
    10741 cgggctgctg atgttaaggg aataaaaaaa gcattgttta actggtcatt atatgttgca
    10801 aaaaagaata ccccatatgt ttgccaggat aaaaagccaa aaggaatatt gggttttcag
    10861 gtacggttgg ctgataaact tatctggtct aagttaaaag ctgcattagg atttgacagg
    10921 atacgaatag ctgtatcagg tggcggaccg ttagcagttt cagatgcaga atttttcctt
    10981 gggatgggta tagttatact tgagggattt ggccttactg aaacaactcc ggtaaccaat
    11041 gtaaacaggc caggtatgat acagccagga tcagttggac ctgctttaaa agatacaatt
    11101 attaaaattg cagatgatgg ggaaatttta ataaaaggtc cacaggtaat gaaagggtat
    11161 tataaaaacc caaaagctac aaaagaagtt ttcaccaaag atggtttttt caaaacgggt
    11221 gatattggtg tcattgatga aaaaggaaga ttgtatataa ctggcagaat taaagatatc
    11281 attgtgactg caggtggtaa aaatatttcc ccacaaaata ttgaaaatag cctgaaagaa
    11341 tcaaagtata ttgaacaggt agcagtaata ggggataggc gaaaatattt atccgccctt
    11401 gttattccgg cttttgagga gcttggcaaa tgggctaaaa aacaaggtat acaatttgcg
    11461 aataataaag aactcattga aaatgataag gtaaatgagt taattgctca ggaaattcag
    11521 aaatatacaa aacaattttc acgtgttgag caaataagaa aatttaaact attagaagct
    11581 gaatggagtc aggcaagcgg tgaacttaca ccaacattaa aagtaaaacg ccgtgttatt
    11641 gaacagaagt atgcaaaaga aatagaatcc atgtatccaa aagatatgga ttaacaatca
    11701 gttgaatata aaacgggcag gttaaccacc tgcccgtttt attattctat taatatcggt
    11761 aatgatcagg tttgtatgga ccatcaactt ctattcccag atatgcggcc tgttttggtg
    11821 ttaatcgtgt taattttact cccaatcgcc ctaaatgtaa tcgtgcaact tcctcatcta
    11881 atttttttgg gagtgtatat actttctttt ccagcttctt tgttgccagt tcaatttggg
    11941 caagtgtttg attggtaaaa cttgtgctca taacaaaact tgggtgaccg ttagcatttc
    12001 ctaagttgac aagccttcct tctgaaagaa caataattga ccttcctgat tccagtatcc
    12061 atttatcaac ctgaggctta atatttattc ttttgcattt tttgttattt tccagatagc
    12121 tcatttcaat ttcactgtca aaatgtccaa tgttgcacac tatcgcctca tctttcattc
    12181 tttccatatg ctcgccccgg ataacatcac agcagcctgt tgcagttaca aagatgtcgc
    12241 caatttctac aacatcctcc agtgttttta cttcataccc ttccattaca gcctgcagcg
    12301 cacagattgg atcaatctct gtaactatca cccgtgcacc aaatccccgc atagattgag
    12361 cgcaaccttt gccaacatca ccgtaaccgc atactactac aactttgcct gcaaccataa
    12421 tacccgttgc acgtttaatc ccgtctgcta aagattctct gcatccataa aggttgtcaa
    12481 atttggattt tgttaccgaa tcgttaacat taattgctgg gaagagaagt tcaccagttt
    12541 tctcaagctg atatagccgg tgaacaccgg tagttgtttc ttctgaaaca cctctgattt
    12601 ttgatgcaat agtagtccac cgtttgggat tgtttgccac tgacaagcgc aatctgtcta
    12661 taaggcattg catatctttg ctgtcatatt cttgatccag tattgatggg tctttttcag
    12721 ctttaacgcc catatgaaca aatagtgttg cgtcaccacc atcatcaaca ataaggtcag
    12781 gcccgcttcc atcaggccat ataagggcct gttcggtgca ccaccagtat tcttcaaggg
    12841 tttcgccttt ccatgcaaag acagcagcag ttccagcttt ggctatagca gcagccgcat
    12901 ggtcctgtgt agaaaaaata ttacaggatg cccagcgaat atctgctcca aggtgatgaa
    12961 gtgtttcaat aagcattgct gtctgtatgg tcatgtgaag gctgccactg attttcatgc
    13021 cttttaatgg tttctggcta ccatatttct gacgtaatgc catgagtcct ggcatttcat
    13081 tttcagctaa ttgcaactcc ttccttccta attctgccag tgaaatatcc tttaccttat
    13141 agggaatttt tgcatcaaat tcaggaatac tggtagtatc tttacgtaat ggctgaatca
    13201 tattacacct cttttgtatt aattttctta tattatgtat tactctatcc aaactagtag
    13261 agtgtatatt gtcaatataa atattatagc aacctttgac gatggtaata gtatgagtaa
    13321 aaaaaattat cgtattttaa tatttgtatt gatgatgaca ttaatttcat gtgtgccttc
    13381 acagtggcaa tacaggaata tacccttaaa aagaaaacaa ataatcaata ctgcaacaca
    13441 atatattggc acaccctaca ggcacggggg tgccacacca tcgggttttg attgttcggg
    13501 ttacgtgttt tatgtgtata acaaaaatgg cattcagata ccacgaagta cagcacaaca
    13561 atatacaaaa gggcaaaaaa taccttataa aatggcccag ccgggggatc tactcttttt
    13621 caggattaac atgaatacaa tatcacatgt tggcatttat gttggaaatg atacatttgt
    13681 acatgctccc agcagcggaa aatatgtgcg gtatgattcc atcaataata catactggaa
    13741 aaagcgtttt tgtggagctg tctcctattt taaagctttg cctgtaaaac ctttaaataa
    13801 taagggcgat agttatcggg aagagcgctt tgtgtggtag aaaaaaatat tgaaatatga
    13861 ataaatgttg acaaaatgta attataagat tatttttgtt gaaccgatca ggataggtta
    13921 gcattatatg caaattggaa tttatccggg atcttttgat cctttaacgt gtggacacct
    13981 ggatataatt aaaagagcaa cacagttgtt tgataaactt atagttgcca ttgctcgcaa
    14041 tagcgaaaaa tttcctctat ttgcagttga agaacgcctt gcaatgctac aggaatgttg
    14101 cagggatata tctaaagttg aaataacaag ctttgatggt cttcttgctg agtattgcaa
    14161 gaagaacaat attaatttta ttgttagagg attgcgggcg atagttgatt ttgaatatga
    14221 atatgccata gcattaatga ataaggaatt ggcgccttca gtggagacta tctttttaat
    14281 gtctaagagt gaattctcat ttgtttcatc caagatggtc aaagaggttg ccagctatgg
    14341 tggtgatatt tccagactgg tcccacagtt tgtcagttca aaacttaagg aaaaattcca
    14401 atctcaataa cctgtcctga ggctacatcc gtagcatatg taattgcagt taaacatcag
    14461 gtaaggagga ttactatgaa cataattact atcctcctga ttgttgctct gcctatagcg
    14521 gcattgttag gctatttgtt cagagcatac atagggaaga ttaagcttaa cactgctgaa
    14581 gcgcagtcac gaaaaatcat tcaggatgct gtcagagaag cagaagctaa acgaaaggaa
    14641 cttctgctgg aagcaaaaga tcagcttctc aaagaaagaa accagtttga aaaagaaatg
    14701 cgtgaaagga ggattgaatt acaaaatatt gaaagaaaaa ttcttcaaaa agaagaaatg
    14761 atcgacaata aaatccagca actggaaaaa caggaaaaat taatacaaat taaggagaaa
    14821 gaaaacttag aaaaagagca ggaattgaat aaacacctgg aaaaacacaa gcaggagtta
    14881 gagcgtattt ctggattaac ccgtgaagaa gccaaacaac tgttgcttaa aaatcttgaa
    14941 aatgaggtta gatttgaggc aataaaatta atcaataaag ttgaagagga agcacgaaga
    15001 acagctgata aaaaagcaaa agagatagtg ttagctgcat tgcagcggag tgcatcagat
    15061 tatactcagg aaagtaccat taccaccgta tctctcccgt ctgatgatat gaagggtagg
    15121 attattgggc gtgaaggtag aaatatccgt actcttgaga atcttacagg tgttgatatg
    15181 attattgatg atacgcctga agttgtggta atttccggtt ttgatccaat acgaagagag
    15241 attgcacggc tatcacttga acgattaatt gaaaatgggc gtattcatcc tgcacgtatt
    15301 gaagaagtgg tagaaaaggt taaaaatgaa ttggaagaaa atatgctgga agaaggtgaa
    15361 cgtgctgcat ttgaattagg tattccaggt ctctcaaaag atgcattgta tcatgtagga
    15421 aaactgaaat atagatcaag ctatggtcag aatgtacttt cacacagtaa agaagttgcc
    15481 aatttagcag ctattatggc tggtgagctg aagcttgatg ttgctacagc aaaacgggca
    15541 gggttattgc acgatatagg taagggaagc attgttgaag gtgaaggcgc tcatgccatt
    15601 gttggggctg agcttgccaa aaaatttggc gaaaatgatg ttgttgtcaa tataattgcc
    15661 tcacatcata atgataagga acctgaatca tttgaagcca tactggtaca ggtagctgat
    15721 gccatttctg cttcacgtcc cggagcacgt cgcgaatcac tggatacata tcttaagcgc
    15781 cttgaaaatc ttgagaacat tgcgtatgga tttaagggtg ttgaaaaatg ctatgcaata
    15841 caggctggaa gggagatcag agtaatggtt tccaatgaac aggttactga tgaagaggct
    15901 actgtactgg cacgggatat agcttctaag attgaatctg aattaaaata tcctggaata
    15961 gtgcgggtta ccgttatacg ggaaacacga atagtagatt atgctcgata agtataaaaa
    16021 caatcagcaa tcagtgacag ctgattgctg attgtaataa taaatagtat ggatacaatc
    16081 aatatattac ttataggtga tattgttgga agacctggac gaacaattat taaagatcac
    16141 ctcagtgaaa tttgcaaaga atactcagta cattgtgtaa ttgcaaattg tgaaaatgct
    16201 gctggaggca tgtccataac acctgagatt gcacaggaac ttttctcgta tggcatccat
    16261 atactgacaa caggaaatca tatttttaat aaccgaacca taataaaact tcttgaatca
    16321 cacagttcta ttttacgtcc tgcaaatttc ccatctggtg tacccggaaa aggatttacc
    16381 atagtaagaa ttaataattt aaatatagca atagtcagtt taattggccg aatcaatatg
    16441 gaacctgtag attgcccatt tattacattt aatacacttt atgatgaaat tagatctaaa
    16501 gcaaatataa ttattgtaga ttttcatgct gaagcaacct cggaaaaaaa agcttttgga
    16561 tggtttgctg atggcagggc atctgctgtt tgtggcaccc acactcatgt tcagactgct
    16621 gatgaaacaa tacttccaca agggactgca tatataacag atgttggcat gacaggtcca
    16681 tttgattctg ttattggcat ggataaagaa tcttcaatac ataattttat atatcatacc
    16741 agaataaaat tcaaagttgc acagaatgat cctaaattaa atgcagtgct tgtagtttgc
    16801 aatagacagg gaaaagctgt atcaattaca cgcatagtta aatcatatga actatcgcca
    16861 agtaattctt gacatttgta aattaaaatt tactaatact attcagaatc gtgtggtata
    16921 ttgaatataa ctaacattca taaaagatta ttatgctaag cgatatatac gggccacagg
    16981 acataaaacg tttgtcagta aaagaacttg cagctctggc tgatgaagta agacaacgta
    17041 ttcttgaggt tgtctcaaag aatggcggtc atttagcttc atcgcttggt gttgttgaac
    17101 tgaccatagc attgcactat gtctttgatt caccacagga tagaataata tgggatgtgg
    17161 ggcatcagtg ctatgcacat aaaattttaa ctggcagaaa tcaacaattt catacattac
    17221 gaacgttcaa tggaattagc ggttttccca aagtttcgga gtcgccacac gatcattata
    17281 atacagggca tagcagtaca agtctttcgc tggcattagg agaagccatt gcacgggatg
    17341 taaataattc aaaatataat attgtagcag ttattggtga tggttcaatg actggtggga
    17401 tgtcatttga ggcattgaat catataggtc atcttaaaaa agatataata attatattga
    17461 acgataatga acattcaatt tcaaaaaacg ttggcgctct ttcagaatac ctgatgcgcc
    17521 ttattactgg tggaacatat aataaattaa gaagaaaatc atacgaattt atcagcagaa
    17581 ttccaaagat tggacccaaa ctttttacct tatttgaccg tattgaagca cgtataaaag
    17641 gaatttttat tcccagcagt ttctttgaag atttaggcat acggtatttt ggcccaattg
    17701 atggtcataa tatacctatg atgatagaga tgtttaacag gatttctaaa ctcaataaag
    17761 gcccaaagat cattcatgta tatacaaaaa agggcaaagg ttatgtacat gcagaaaata
    17821 atcccgcaaa atttcatggg ataggcccat ttgatataaa aacgggaaaa ccagttaaga
    17881 aattatctat cagttattcg gagatagcgg gcaggactct ggttgattta gccaagcatg
    17941 ataaaaaaat atttgctatt acagctgcaa tgaaactggg gactggttta gctgaatttg
    18001 aggaaaaatt ccccagccga tttattgatg ttgggatatg tgaacagcat gctataacac
    18061 tggcatcagc actggctaaa aatggattga aaccttttgt atcaatatat tcaacattta
    18121 tgcagagagc cgttgatcag attattcatg atgttgcgtt gatgaatttg cctgttaaac
    18181 ttctcataga tagggcaggt atagttgggg atgatggtga gacacatcat gggctttttg
    18241 atattggtat tatcaggtct ataccaaatt ttgttttatt atcgcctacc aatggtaatg
    18301 agcttaaaga tatgataaac tatgctgcca gttataacaa tgggcctgtt gccatacgat
    18361 atccacgagg tggtgatgat aaatatgaac attcaatgcc aaaaggatta ttgaaaattc
    18421 ctcaattgaa agcgtactat aaaagccaac gcccaatgat tgctctattt gctgccggag
    18481 atatgttatc tatggcagtt gaaattcata aaaagttaca tgcatataat attaaatcat
    18541 cagtgtatag cattctatca ataaaaccat taccggtaaa acaaatagaa tttataatga
    18601 ataccatacc atactatatt gttctggaaa acagttatgc taatggtggt atagctgaat
    18661 acataaattc acagcttgac agatcaatgc ggcataaaaa tctctttaat gttgcatttc
    18721 cagatagttt cataacacat ggcaaagttt ctgagctatt acgctattat gagcttgatt
    18781 cagatactat aacacataaa attatcagca tgattaatac agagaaaaat tctcatgcca
    18841 aaaaaccgct tagatcaata tcttcttcaa aataatatct gcacttcacg agaaaaggcc
    18901 aaaaatttaa ttatagctgg ttatgttaca gtgaataacc aggtaatcta taagccatca
    18961 ttcatagtaa aagaaaatga tgttataact gttcgtgaat tgtcacagaa atttgtaagc
    19021 cgtggtggtg aaaaattaaa gaaagctctg gattatttta acattgatgt aacaggaaaa
    19081 attgtactgg atttgggctc atctactgga ggatttgttg attgcctttt acaatacgga
    19141 gcagaaaaag tatatgcagt agatgtggga tatgggcaac tggattataa cctgcgaatt
    19201 aatccaaagg ttattgtcat ggaacgaaag aatgcacgct atttaacaat agaagatttt
    19261 gatgatcata tacatctgat aacagctgat ctctcattta tatcaataat caaagttatg
    19321 cagacaatga taacaatatt tgattatgaa attgatgcaa ttcttctgat aaaacctcaa
    19381 tttgaagctg aaagctttca acataaaaaa ggagtggtaa aaatgcccca atatcataag
    19441 gacatacttt taaaggtttt tcaacaactt caaacaatga atataacaat acttggatta
    19501 acctattctc ccattaaagg gcccaaaggc aatattgaat ttttattata tggtgcaatc
    19561 aattgtaata ccaggaaatc agttgataat tatgagcttc ttatcaattc atgtgtagat
    19621 gaagcacata aagtactgta ataattcaaa tttttaatag tatataaata tattaattga
    19681 cgaatactgc cctgtagttc attataaatt atgtttttga ta
//
LOCUS       DAORYL010000057        19471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_2353_length_19471_cov_11.915225, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000057 DAORYL010000000
VERSION     DAORYL010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 19471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19471
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2353_length_19471_cov_11.915225"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLV81_10555"
     CDS             complement(<1..218)
                     /locus_tag="PLV81_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10578.1"
                     /translation="MNKKKINILIINYEFPPLGGGGGVATYDLALEWVKTANVHVITS
                     SFKGLPSYENINGIHVHRVKILFRKSRDA"
     gene            complement(445..591)
                     /locus_tag="PLV81_10560"
     CDS             complement(445..591)
                     /locus_tag="PLV81_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10579.1"
                     /translation="MVSQEKEMKYWTVRFIVQICIVPGNGGLWKTPTGYIDIFLPKKK
                     VLLM"
     gene            complement(840..1031)
                     /locus_tag="PLV81_10565"
     CDS             complement(840..1031)
                     /locus_tag="PLV81_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10580.1"
                     /translation="MELLIYNEEKELSSKKCFIANEYRYGKYCILGRQHATISREIKQ
                     NATHRYNEFESEEKAHPKE"
     gene            1368..1988
                     /locus_tag="PLV81_10570"
     CDS             1368..1988
                     /locus_tag="PLV81_10570"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOM10581.1"
                     /translation="MKFLHVLGVIITVSSLFACSTIDVAYTNKQAIQKRMKRVAVFPF
                     DIKGAGWGDEFSDAISHQFFKSGKVTTIEREAIQKILQEQQLSASGLIDDKQAVKIGK
                     LLGADVIIIGRGSALQAYRNNKFEPNCIDTFTLKAINTENGEILLTVRKEPGRAWDWR
                     YRAKFCCGLTLIWNLNDILIESSQYDDIAKQVVKQILAILQEENKR"
     gene            1985..2764
                     /locus_tag="PLV81_10575"
     CDS             1985..2764
                     /locus_tag="PLV81_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10582.1"
                     /translation="MKWKSKTYIRILILVSVIAFFSSAYSSEFDELDKPPEGIHKDQI
                     LLTGSVSIGVPYGDLISAEEKFTKTSTYTFENEITKLIYLSHLAFGLTISGEYLPIDY
                     IGIRSKIRRSVIVQNTNFGADYQSWKKTLYSDYSVYFGPSFHVTNRKPWDISLSPMLG
                     YSFATYKPTPIARILIDGYTPPPTQQFSSITYGVDVMLSIFFSGGLVVQIGGEWIRNN
                     IIFSSIPDAVNPQTNQTFYTKKDATIDSILFCVSAGYAFYN"
     gene            complement(2889..3878)
                     /locus_tag="PLV81_10580"
     CDS             complement(2889..3878)
                     /locus_tag="PLV81_10580"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOM10583.1"
                     /translation="MEIQSKYLNGDIIQWLMDDAVSLFFLKKRIPALQETTIQKDSIQ
                     IVHSASLFSPNRKTFEYFDTPGIGIMWFFSSLVESGFDISDNVVYSILKEILTFQQDD
                     GGFTLNWKPFCSTACITGQLVYSLLESGYQGKEVEKGIAWILAHQRDDGGWLHCPIGS
                     VGDSLNYLLFKRTGGGIRLEHTDVPSCAVATVMCARALMKTGNQISNDTLHRAIAYMF
                     RNDAIFKETGCRCRISYNHELLGYPFYLDYDSIDAIQFSLYLGNGSETFRIRLFNQCI
                     KKQFEDGSFPLEHRRRGCMHQFLQLPVKLKSKDKLTTVRVISMLVNTGNAVIK"
     gene            complement(3894..4616)
                     /locus_tag="PLV81_10585"
     CDS             complement(3894..4616)
                     /locus_tag="PLV81_10585"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM10584.1"
                     /translation="MRLFFISAFILTIPMMLYADYNKALKLFEQGKYDDALTEIASVL
                     DVSRDFETNTPNYNLRYLAGHIHAKKGNYESALTHLKRCAEIQKDTINPLIDIAFIYI
                     DSKRYTDAMTWARKALQIKQEPVAYYILGLAYSGIGSYWQAKEYLEKAISLDPEMYYA
                     YNELGNVLMALGRITQAQTAYSAAYALAPSSAYICNNLAMSYVQAGDVKKAIEIIEKA
                     RKLAPDNPIIVENYNHIVSLKK"
     gene            complement(4765..7479)
                     /gene="ppdK"
                     /locus_tag="PLV81_10590"
     CDS             complement(4765..7479)
                     /gene="ppdK"
                     /locus_tag="PLV81_10590"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOM10585.1"
                     /translation="MAKRVYLFGGNVTEGTADMKNLLGGKGANLAEMARIGIPVPAGF
                     TITTEVCNEYYKNNQKMPDGLKEEVIAALRKVEEIMGTKFGDPSNPLLVSVRSGARAS
                     MPGMMDTILNLGINEDVVKGLVTKTGNERFAWDSYRRFIQMYGDVVLGLKPESKDEHD
                     PFEVIMDELKEKRKVKNDLELTVDDLKELVARFKKIIKDKLNVSFPDDPYEQLWGAIG
                     AVFRSWNNERAILYRKMNNIDDSWGTAVNVQAMVFGNMGNDCATGVAFTRDPATGAKE
                     FFGEYLINAQGEDVVAGIRTPQQITLEGSRRWARENGVSEEERKQKYPSLEEYMPEAY
                     KQLVDVYRTLEKHYRDMQDIEFTIQNKKLWMLQTRNGKRTARAAINIAVDMVEEGLIT
                     KEDAVLRVDPQSLDQLLHPTFDPKAQRKVIAKGLPASPGAATGKIVFNADDAHEWKER
                     GEKVILVRIETSPEDLKGMNAAEGILTARGGMTSHAAVVARGMGKCCVSGCGVLEIDY
                     AKRTMKVEDITLKEGDYISIDGSTGEVMLGKVATVEPEVTGKFGTIMQWADEIRKLKV
                     RTNADTPHDATVARNFGAEGIGLCRTEHMFFEGDRIKAVREMILADTFEGRKKALDKL
                     LPMQREDFYGIFKAMEGLGVTIRLLDPPLHEFLPHDLESQKEMAKELGITVEDVKAKV
                     EELHEFNPMLGHRGCRLGITYPEITEMQARAIFEAACQLVKEGVDVKPEVMVPLVGHV
                     KELIMQKDIIVKTAETVMKEMGVKVDYMVGTMIEVPRAAVTADEIAKVAEFFSFGTND
                     LTQMTFGFSRDDAGKFLKEYVDKKILPNDPFRILDRDGVGQLIKMAYEKGRSVNPKLK
                     LGICGEHGGEPSSVEFCHMVGLDYVSCSPFRVPIARLAAAQAAIRFPK"
     gene            complement(7672..8601)
                     /locus_tag="PLV81_10595"
     CDS             complement(7672..8601)
                     /locus_tag="PLV81_10595"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOM10586.1"
                     /translation="MYKNWAVTLIIANGIIFLIQLLLSNTLVPYTVANNVVPMDIVTY
                     YLGLTPYLVVDKFYLWQLFSYMFLHSTSGFAHIIFNMYALFIFGMPVERLWGSKKFLI
                     YYLTCGIGAGLAIFIIALVSGGLGYYIPTIGASGAVFGLLLAFGILFPDVEILLFFII
                     PVKARTLVILYGALELFLELSGGFDNISHIGHLGGLATGILYFIITRKHAIRYKTRGF
                     VALQKKKATIPAEHVVDKKEIELQKTIVQKLYKQGEQALTDDEVQYIRYKEIMVETNT
                     VDCDISIFTSNSCENCIDRDACFVKIVKDLLKQ"
     gene            complement(8636..8953)
                     /locus_tag="PLV81_10600"
     CDS             complement(8636..8953)
                     /locus_tag="PLV81_10600"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOM10587.1"
                     /translation="MQKKECRRCGACCFVDMIAYATEDDFVRWRAEGRHDILHIIENE
                     ASIWAGDHLISSRNGHSIQRCPFLIMDDDGLYSCTIYNTRPAVCRDFEPGVSRMCPQY
                     EDQ"
     gene            complement(8978..9568)
                     /locus_tag="PLV81_10605"
     CDS             complement(8978..9568)
                     /locus_tag="PLV81_10605"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOM10588.1"
                     /translation="MPDVVIKENSKQKTLIMQKSQVLFWEKGYAETSMKDIANECGFR
                     PANIYNFFESKEEILFSILKEEMEEIVNPIRYLEKEDGDPLQQLRLLIETHVKITLGE
                     KRASKLLFDTELRNLSPERRKVILNLRDEYNRIGYSILRRGIEKGLFRKVDEKIALNL
                     IASMIVRTRLWFSPEEWMTIDDLIEFIYQFALKGLS"
     gene            complement(9778..10548)
                     /locus_tag="PLV81_10610"
     CDS             complement(9778..10548)
                     /locus_tag="PLV81_10610"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOM10589.1"
                     /translation="MSDAVLLEQKGMVGILTINKPKANQLSHDVFETMRKLLDTLEID
                     KNIRVLVITGSGDKIFCAGADLSQGFGDFSAVDFLKRGQDVWNKIEDYPKPVIAALNG
                     HALGGGCELAMACHIRLMKKGARIGLTETNLGIMPGYGGTLRLPRLVGRAKALEIMLF
                     GKQLEADEALAIGLVHKVFEEQDFMGEVLKFAEELAARPPLSVKAILKVLSAGPSMSP
                     EMHLKMEREELAKLFTTKDCMEGMMAFAQKRKPEFKGE"
     gene            complement(10559..11722)
                     /locus_tag="PLV81_10615"
     CDS             complement(10559..11722)
                     /locus_tag="PLV81_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOM10590.1"
                     /translation="MQSINDVKKIAVIGSGAMGHGIAQVCAQNGLMVTMIDIKQEFLD
                     NALKKVKESLDFLASKGKLQGTVDEILGKISVTTDLQKGVSDAQVIIEAVPENMQLKK
                     DVFAKASSACASDAILATNTSTMSITEIATAVTNPERFAGMHFFNPVTRMKLVELIYG
                     AKTSENAINILFELTKKIDKFPVKVLKDRPGFIVNRISAPNQALLSAILDEGKIHPAQ
                     IDGKMKKMGVKMAPFETADFVGLDVFCHTLEYYAQTLSPQYKPGKFLLGCLDKGHLGM
                     KSGKGIYEWKDGKAIIPEMPDTEEITPLHLMAVQVNEAVKVYKEGIAASVQDIDDGVK
                     YGMNAFAGPFALASGVQPQQLTDCLNYLAQRFKLDILKPEPEIIDGSFKTIGR"
     gene            complement(11749..12945)
                     /locus_tag="PLV81_10620"
     CDS             complement(11749..12945)
                     /locus_tag="PLV81_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOM10591.1"
                     /translation="MIQLVTPDYIKTLQDNMHKLAEGVLRPISRKYDEAEHEYPKELD
                     MFRGIKVMARPKKKKEGESSASGEEKKPKRGANLGMVVTIEEMCWGDAALLLSFPNAG
                     LGNAAILAVANDEQLERFGNKWAAMAITEPGAGSDSGAITTTATRDGDYWILNGEKIF
                     VTCADRSEVVVVWATVDKKAGKAGIKSFVVEKGTPGFTLEHLEHKLGIRASDTGTFVL
                     SDCRIPYNNILGDPEVKMSTEGFKGVMKTFDNTRPLVASMATGIARAAVEFTREQYEK
                     KGYAFDYKKNLSNVSAVEREWYLMDANVEAMRLLTWRAAWMADNNQFNNLEASMAKAK
                     AGRYATLITQRCSELLGPVGYSTQYLAEKWMRDSKIMDIFEGTGQIQHLIIARNVLEM
                     SSKELK"
     gene            complement(12965..14062)
                     /locus_tag="PLV81_10625"
     CDS             complement(12965..14062)
                     /locus_tag="PLV81_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOM10592.1"
                     /translation="MVEEISFALSEEQQMMQDTVRQLVQANVIDAIHQWDENRLLDKQ
                     AVQKFWELGIIQSMIPEEYGGYGMGSSPVLHTLVLEELAFGDMAFAVYAMLPALFVLP
                     VVNLGSTEQKEKYLPEFCKESFVPATLAVNELSVGFDVWNCETKAEKKGNTYVLNGKK
                     CFVPLADEAKHMIVIAHYEGNPHLFIVDKDAKGLSVGEREKNCGWWALPTFEVTFENC
                     TIPEISKLGDETAFNWLIQRTRTAMAAIGTGISRASYEYSKKYAKERQQFGEPIASRQ
                     SIAFMIAEMAYEVDAMRLLTWKAASAIELGMDANRESFLAKIYAGEMTMKLTDYGVQI
                     LGGHGYIRDHPVERYYRNGRSIAICEAMAII"
     gene            complement(14131..14745)
                     /locus_tag="PLV81_10630"
     CDS             complement(14131..14745)
                     /locus_tag="PLV81_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOM10593.1"
                     /translation="MTRSDDTRDKILLHAKRLFSEKGYYATQISDIIESAKIGRGTVY
                     QYFSNKEHLFISLLERFLIEWERYINELSIKEDLETISPYQYLKKRLYEAFRFFFEDR
                     DRANIILRAGLGLPDHFEQKIQHFENKLINMIIADLKIAVNFSNISKEADLNFMANLI
                     AGGVFRLAHYYLTITMDDNMLIEDLETISDKASRIIAQGIFNKK"
     gene            14948..15505
                     /locus_tag="PLV81_10635"
     CDS             14948..15505
                     /locus_tag="PLV81_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOM10594.1"
                     /translation="MVIAPNTSIDQFLTEIMPKVATEMLQNSNAPKELGGTEITMVVE
                     VNGKSYGFKVKDGKDIETCGDIANAMLRLKISEADLKKMIETNNLDMLLGMQKDLTRA
                     RYNALSSLKGSFIAKLKNDDGSEFTIEAILNGATSPQSIFRMTTKDSAALMKKETNPV
                     NLFMSGAMKIEGDMSFAMATQPLFT"
     gene            15580..17457
                     /locus_tag="PLV81_10640"
     CDS             15580..17457
                     /locus_tag="PLV81_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOM10595.1"
                     /translation="METLPWYKEYKVLGIPKTFKPYPDKPVYDILEIAAKKYKKNGLL
                     QKDYFISYPQLLDYVNRLASRLQSFGLIKNDIIATILPTSTEFIIADYAISKAGLIHL
                     PCSSLEPAEALEHKFKEGKPKAIICTSNDIKILTSLIKKTSVRAIIIANTRTSNSFPT
                     IDTDKIKISGSTVYDLYSLIQNSSGNVNPVPINVETDIETLIFTGGTTGVPKGCMLTH
                     RNIYANCIQNLHALGAGGLTFKGAISVILGLPFFHTYGHCLMHTMMLFGFNIIIINDP
                     RDTKSMVENIKKHYPVMQIGVPAQFMKLAKEDLSGIGIIGLSGSAPLSESTQEEFEKR
                     SNSGIMEGYGLSEMSPVTHLNPSVIYRILGGRFGVRLVSLLSSIPGNSYLTNLLFRSI
                     GTKNFGWIFTKIIAYRMKKTSQKPGKKSVEKRRTIGVPFPDTEVRFLDFDSGNILTID
                     DMLAGKRAEMLLKGPQRMLGYWPKPDSGCDAEGFIHTGDIVQIDSNGYFYIVDRSKDM
                     INVSGFKVYSKEIDDIAGEHPHIEAAATVGIPNPEKEGSEVVVLFVQLYPQYKNKTKE
                     QDILKYLQSKVAKYAVPKFIKIVDELPRTAVEKVDKKILRQMAIDQFLISKKKVKIKT
                     K"
     gene            17483..18661
                     /locus_tag="PLV81_10645"
     CDS             17483..18661
                     /locus_tag="PLV81_10645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOM10596.1"
                     /translation="MRECVFIDGVRTPNGRAHREKGWFRHLRPDELLTPVYNALFERN
                     SKVAPDMVDAVFVGCANPSGMQNDIGRLAWLASGLPDSVPTNTLTNQCPSGMSATMHA
                     ARAILTGEADIIIAAGVEDMEKVPMGANMDFPPRLLKYYNGADLLMGATAEKVAEQYN
                     ISREDMENMAVWSNKKAWEATIAGKFKKEIVPIKGLDDNGNEFLVDRDQWVRDKVDVE
                     KMKTMMSPFKPNGVVTAATSSPLTQGACALLLMSREKADKLGLPYRYKYSYGVLAGCD
                     PTVMGIGPIPAIKKLVARTGLDLDKVGAIELNEAFASQSLACIRELKLDNDNAPFKRV
                     NVWGGAIALGHPLGESGARIIITLLNVLSTEFPDAQYGLASLCGAFGNAGALLVEKVN
                     "
     gene            18802..19443
                     /locus_tag="PLV81_10650"
     CDS             18802..19443
                     /locus_tag="PLV81_10650"
                     /inference="COORDINATES: protein motif:HMM:NF013972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HOM10597.1"
                     /translation="MKRLLIISLTIIFASISILVFSQQSNWKLVHESKEDQASLQIFT
                     RDIPGSPYKEFKGVSLSDIPFDIALKVIQDYNNYHKWYGMCNALYPINVRNPKDLDMY
                     FILDLPVVKDRDVVVKVECGWDEKKGTAWVNMTSINDSPYKNDSGLVRMTSLKGRWDI
                     IKKDENHVEVIYQVHAELGGSLPAWVVNIAAKDQPLKTLKGVYKYCKKIRDNK"
BASE COUNT         6031 a         4092 c         3069 g         6279 t
ORIGIN      
        1 gcatcccttg attttctgaa taaaatcttt accctgtgca catgaatccc attaatattt
       61 tcataggagg gtaacccttt aaatgatgaa gtgataacat gcacatttgc agtttttacc
      121 cactccagcg ccaggtcata ggttgcaaca cctcccccac ctcctaaggg aggaaactca
      181 taatttataa ttaatatatt aatctttttt ttgttcatgt aaaatactcc aattcaaaca
      241 aaaaaggctt attttaactc acactacaac atgcctttta tatttcaaat actatttact
      301 cctgaaaagc taaaacaatg cagatacatt tttgcagtgc accatgtagt tttccctata
      361 aaacctcata caaagatttc catacattag atttacccgg agttgtatgt atcattatag
      421 ttatgctaac tattccctca tcgcttacat aagcaaaact tttttctttg gcaaaaaaat
      481 atcgatataa cctgttggtg ttttccacaa tcccccgttg ccaggcacaa tacatatctg
      541 cacaataaat ctaacagtcc agtatttcat ctctttttcc tgtgaaacca tacattgaaa
      601 ctatacaagc cttcccctgc actttctttt gtccagacgg caacaaaaga aagtgccaaa
      661 gaaaaatgcc tttaccttcc tgtatctatc taatccttgc cagcgtgctg cggacgccct
      721 ctaaggcttt tgtcctcgca cgcaacatat tacgccgtac ctcctgtacg gctatcattg
      781 acccttactt ttgcttttat caacctttgc tgttttttaa aagaacgctt gaatctggtt
      841 tattcctttg ggtgtgcttt ttcttctgat tcaaactcat tatatctatg tgttgcattc
      901 tgctttattt ctctgcttat tgttgcatgt tgtctcccta atatacaata tttcccgtaa
      961 cgatattcat ttgctataaa gcattttttt gatgacaatt ctttctcctc attatatatg
     1021 agtaattcca catacatacc tgctttttcg tttttgttgg tggaacatcc actataaagt
     1081 atatgttgtg tatgaggcta cattttttta ttttgtgttg catgttatcc atgaatctgc
     1141 acaatggctt ccctttcaaa gcagcacatg ccataaaaat acattccttg acaaacatgt
     1201 actataaatt aaaaattaaa aaataatttt ttgttaaaaa ataatatttg tcaaaatcca
     1261 ttataagatg gtactatact atactatacg gtaagcggta ataaaacaac atcattatag
     1321 agtaaaaaga ttttattttt tatattataa aacaagggtg gaaaattatg aaattcttac
     1381 atgttttagg tgttattatc accgtatctt cattatttgc ctgttcaacc atcgatgtag
     1441 catataccaa taagcaggca atacaaaaaa gaatgaagcg ggttgcagtt tttccctttg
     1501 atataaaagg ggctggatgg ggcgatgaat tttcagatgc tatctcacat caatttttta
     1561 aaagtggaaa agtcactacc atcgaacgcg aggccataca aaaaattttg caggaacagc
     1621 agctttctgc ctctggttta attgacgata aacaggctgt aaaaattggt aaattgttag
     1681 gtgctgatgt aatcattatt ggccgtggct cagctttaca ggcatacagg aacaacaagt
     1741 ttgaacccaa ctgtattgat acgtttacat taaaagctat taatactgaa aatggtgaaa
     1801 ttctgctaac cgtccgcaaa gaaccaggga gagcatggga ctggcgttac agagcaaaat
     1861 tttgctgtgg attaacatta atctggaatc tcaatgatat tctaatagag agttcccagt
     1921 atgatgacat tgccaaacag gtagttaagc aaatcctggc tattttacaa gaagaaaata
     1981 aaagatgaaa tggaaatcaa aaacatatat caggatttta atcctggtta gtgtcatagc
     2041 atttttttct tctgcctatt catcagaatt tgatgaactc gataagcctc ctgaaggcat
     2101 acataaagat cagatactac ttactggctc agtttctatc ggtgtcccat acggtgatct
     2161 tatctcagca gaagaaaaat ttactaaaac ctcaacctat acctttgaga atgaaataac
     2221 taaactcatt tatctttctc atttagcttt tggattaaca atttcaggag aatatctccc
     2281 cattgattat attggcatac ggtcaaaaat acggcgtagt gttatagtac agaatacaaa
     2341 ttttggcgct gactaccaat cctggaaaaa aacactgtat agcgattatt ctgtttattt
     2401 tggcccatct tttcatgtta caaatagaaa gccatgggac attagtctat cgcccatgct
     2461 tggctattca tttgctactt ataaaccaac tcctatagcc cgcatactca ttgatgggta
     2521 tacaccacca cctacacagc agttttcaag tattacttat ggtgttgatg tcatgctcag
     2581 catatttttc agtggggggc ttgttgtcca gattggtggg gaatggatac gtaacaatat
     2641 aatattttca tcaatccccg atgctgtaaa tccacaaaca aaccagacat tctatactaa
     2701 gaaagatgcc actattgatt ccattctctt ctgtgtttca gccggatatg ctttctataa
     2761 ttaatatctt caattgtaaa tacaacacaa gaagataaaa cttcaatttc atatacctta
     2821 acatgcctga atcatgattt aatccatatc atttccattg tttgcattta taattattta
     2881 cttacaagtt attttattac tgcattccct gtattaacca gcattgatat cacacgcact
     2941 gtagtcagtt tatctttact cttcaattta acaggcaatt gcaaaaactg atgcatacat
     3001 cctcttcgtc tgtgttctaa tgggaagctt ccatcctcaa attgtttctt tatacactga
     3061 ttaaataatc gtatcctgaa cgtttcagat ccattaccta agtaaagtga aaactgaatg
     3121 gcatcaatac tatcatagtc taaataaaac ggatatccca acaattcatg attataggaa
     3181 attctgcatc tgcaaccagt ttctttaaat atggcatcat tacggaacat ataggcgata
     3241 gctctgtgta atgtatcatt gctgatctgg ttacctgttt tcatcagtgc tctagcacac
     3301 atgacagttg caaccgcaca acttggtaca tcagtatgct ctaatcggat tcctcccccg
     3361 gtacgtttaa aaagcaaata gttaagcgaa tcaccaaccg acccaatagg acaatgcagc
     3421 catccaccat catccctctg atgagccaga atccaggcta tacctttctc tacttccttt
     3481 ccctgatacc cgctttcaag caggctatat actaattggc cagtaataca ggctgttgaa
     3541 cagaaaggtt tccagtttaa cgtaaatcct ccgtcatcct gttggaatgt aagtatttct
     3601 ttgagaattg agtacaccac attatcactg atatcaaatc cactttccac cagtgatgaa
     3661 aaaaaccaca taatgccaat acctggtgta tcaaaatact caaaggtttt tctatttggt
     3721 gaaaacaaac tggccgaatg tactatttga atgctgtctt tttgtatagt agtttcttgt
     3781 aaagcaggaa tacgtttctt taaaaaaaat aatgataccg catcatccat taaccattgg
     3841 ataatatcac catttaaata ttttgattga atttccacca atcctatagt tgcttatttt
     3901 tttaaactga caatatgatt ataattctct acaattatgg ggttatcagg tgcaagcttt
     3961 cttgcctttt caatgatttc aatagctttt ttcacatcac cagcctgaac ataactcatg
     4021 gcaagattat tgcaaatata tgctgaagaa ggtgccagtg catatgcagc cgaatatgca
     4081 gtctgtgcct gggtaattct gcctaaagcc atcagcacat tgcccaattc attgtatgca
     4141 tagtacatct ctggatcaag tgagatagct ttttctaaat attcttttgc ctgccagtac
     4201 gaaccaatac ctgaataggc caatcccaaa atataatatg caacaggttc ctgcttgatc
     4261 tgcaatgctt tgcgggccca cgtcattgca tcagtatacc gcttagaatc aatatatata
     4321 aacgcaatat caatgagagg attaatggta tctttctgaa tttctgcaca acgctttaaa
     4381 tgggttagag ctgattcata attgcccttc ttagcatgga tatgacctgc taaataacga
     4441 agattataat ttggcgtatt tgtctcaaaa tctcttgaaa catccagaac agatgctatt
     4501 tcggttaatg catcatcata tttcccttgc tcaaacagtt tcaaggcttt attataatct
     4561 gcatatagca tcatgggaat tgttagtata aaagcagaaa taaaaaatag tctcattttc
     4621 acgttttatt ccccccaaaa acattacata cttccctcac ccctaagtct actaattaac
     4681 aaatagaatc aggggtgagg tattaaaaat atatcacttg tacaatgaac cttatcttaa
     4741 tcacaaaaaa ctacatttta ctttttactt aggaaaccga attgcagcct gagcagcagc
     4801 cagtcgggca ataggtactc taaacggtga gcaacttaca taatcaagcc caaccatgtg
     4861 gcaaaactca actgaacttg gctcaccacc atgttcacca caaataccca gtttgagctt
     4921 tggatttaca gagcgtcctt tttcatatgc cattttaata agctgcccaa caccatccct
     4981 gtcaagaatt ctaaatgggt catttggcaa aattttctta tcaacatact ctttcaggaa
     5041 ttttcctgca tcgtcacggc taaaaccaaa tgtcatctgt gtgaggtcat ttgttccaaa
     5101 agagaagaac tcagcaacct ttgctatctc atcagctgtt actgcagctc gtggtacttc
     5161 aatcattgta ccaaccatat aatcaacttt tactcccatc tctttcatga cagtttcagc
     5221 agtttttaca atgatgtcct tctgcattat aagttcctta acatgaccaa ccagtggaac
     5281 cataacctca ggcttaacat ccacaccttc ttttacaagc tggcaggctg cttcaaatat
     5341 tgcacgcgcc tgcatctctg taatttcagg ataggtaatg cccaaacggc aacctctgtg
     5401 ccctaacatg gggttaaatt catggagttc ttcaaccttg gccttaacat cttcaactgt
     5461 tatacctaat tcttttgcca tctctttctg ggattccaga tcatgtggta aaaattcatg
     5521 taatggtgga tcaagtaatc tgatagttac tcccaatcct tccattgctt tgaatatccc
     5581 ataaaaatcc tctcgttgca taggtagcaa tttatccagt gccttctttc ttccttcaaa
     5641 ggtatcagca agtatcattt cccttactgc ttttatcctg tccccctcaa agaacatatg
     5701 ttcagtgcgg cacagtccta tcccttcagc tccaaaattt cgagcaactg ttgcatcatg
     5761 aggcgtatcg gcgttggtcc ttactttaag ctttctgatt tcatcagccc actgcataat
     5821 ggtcccaaat ttgcctgtta cttctggttc aacggttgca actttgccaa gcatcacctc
     5881 tcctgttgaa ccatctatgg agatgtaatc cccttctttt aatgtaatat cttcaacttt
     5941 catagttcgc ttagcgtaat ctatctccag cacgccgcaa cctgatacac agcattttcc
     6001 cattcccctg gcaacaacag cagcatgcga tgtcatacca ccacgtgcag taagtatacc
     6061 ttcagcagca ttcatgccct ttaaatcctc aggcgatgtt tctattctaa ccaagatcac
     6121 cttttcacca cgttctttcc attcatgggc atcgtcagca ttaaaaacaa ttttccctgt
     6181 tgcagcccca ggggacgctg gaagaccttt tgcaataacc ttgcgctgtg cctttggatc
     6241 aaatgttgga tggagtagct ggtcaagtga ttgtggatcc actcttaaca ccgcatcctc
     6301 ttttgttatc aaaccttctt caaccatatc aacagcaata tttatagctg ctcgtgcagt
     6361 tcgtttgcca tttcgtgtct ggagcatcca caattttttg ttttgtatgg taaactcaat
     6421 atcctgcatg tcgcgataat gtttttcaag agtcctgtac acatccacta actgcttata
     6481 tgcttctggc atatactctt ccagtgatgg atatttctgt ttacgttcct cctctgatac
     6541 cccattttcc ctggcccacc gacgggaccc ttcaagtgtt atctgttgtg gtgttcttat
     6601 gcctgcaaca acatcctcac cctgagcatt aatcaggtat tcaccaaaaa attcctttgc
     6661 acctgtagca ggatctcttg tgaaggcaac accggttgca caatcattgc ccatatttcc
     6721 aaaaaccata gcctgaacat ttactgcagt accccagcta tcatcaatgt tattcatttt
     6781 tcggtacagg atagcacgct cattattcca tgatctgaaa actgctccta ttgctcccca
     6841 taactgctca taggggtcat cagggaatga tacattgagc ttatctttta taattttctt
     6901 aaatcgcgcc accagttctt ttaaatcatc aacagtaagc tcaaggtcat ttttaacttt
     6961 ccttttttcc ttaagttcat ccataattac ttcaaatgga tcatgctcat ccttgctttc
     7021 cggcttcaat cccagtacaa cgtcaccata catctggata aagcggcggt aagaatccca
     7081 tgcaaaccgc tcatttcctg tttttgttac aaggcctttt accacgtctt catttattcc
     7141 taaattcagg attgtatcca tcattccagg catagatgcc cgtgcaccag atctaactga
     7201 caccagcaaa gggttggaag ggtcaccaaa cttagtgccc ataatttctt ctacctttcg
     7261 taatgcagct ataacttcct cttttagtcc atcaggcatt ttctgattgt ttttataata
     7321 ttcattacat acttctgttg taattgtaaa gcctgcggga acaggtatgc caatacgtgc
     7381 catttctgct aaattagcac ctttaccacc taaaaggttc ttcatatcag cagttccctc
     7441 tgtaacgtta ccaccaaaca gatatacacg ctttgccatt gttgttctcc cttcttattt
     7501 agatttatta ttttgccacc ataaaaatgg taacatatct catttccaaa ataaatacag
     7561 aatagcaggc tatttttacc agcaatactt accggtttaa catccttata gtactacatt
     7621 tctgttgtaa agattaattt caaaatacta tcatcttttt caagatatat atcattgttt
     7681 gagcaaatct tttacaattt taacaaaaca tgcgtctcta tcaatacaat tttcacaact
     7741 atttgacgta aaaatactga tatcgcaatc tacagtattt gtttcaacca taatttcttt
     7801 ataccgtata tactgcactt catcatctgt taacgcttgc tcaccctgtt tgtataattt
     7861 ttgtacgata gttttttgta attcaatctc ctttttgtct acaacatgtt cagctggaat
     7921 ggttgccttt ttcttttgca aagcaacaaa ccctctggtt ttatatctga tagcatgttt
     7981 gcgggtaatt atgaaataga gaataccagt ggcaagccca cccagatgcc ctatatgtga
     8041 gatgttatca aatccacctg atagctccag aaacagttcc agagcgccat acaatatcac
     8101 cagtgttcgt gctttcaccg gtatgataaa aaaaagtaat atttcaacat caggaaacaa
     8161 tatgccaaag gccagaagaa gcccaaaaac tgctccagaa gcaccaatag ttgggatata
     8221 atatcccagt ccacccgata ctaaagctat gataaaaatt gctaagcctg ccccaatacc
     8281 acaggttaaa tagtatataa ggaatttttt gctcccccac agtctttcaa caggcatacc
     8341 aaagataaaa agagcataca tattaaatat aatgtgtgca aaacctgaag tgctatggag
     8401 aaacatatac gaaaatagct gccatagata aaatttatca actaccagat aaggagttaa
     8461 acccaggtaa tacgtaacaa tatccatagg tacaacatta ttagcaacag tatacggaac
     8521 aagagtatta ctcaacaaaa gttgaatgag aaaaattata ccatttgcta ttataagggt
     8581 aacagcccag ttcttataca ttaaccaatc ctgctctttc tattacgtaa ttttattatt
     8641 gatcctcata ttgagggcac attcttgaaa cgccaggctc aaaatccctg caaactgccg
     8701 gtcgtgtgtt ataaatggta caactataga ggccatcatc atccattatt aaaaaaggac
     8761 atcgttgaat tgaatgccca ttacgggaag aaatcaaatg atcaccagcc catatgctgg
     8821 cttcattttc aattatatgc aaaatatcat gccgcccttc agcgcgccag cgtacaaaat
     8881 catcttcagt tgcatatgca atcatatcaa caaagcaaca tgcaccacat cgcctgcact
     8941 cttttttttg catagtattt aaattattat agtttgccta tgacaaccct ttcaacgcaa
     9001 actggtagat aaattcaatt aaatcatcta ttgtcatcca ttcttccgga gaaaaccata
     9061 accgtgttct aacgatcatt gaagcaataa gattcaaagc aattttctca tctacttttc
     9121 tgaataatcc cttttcaatt ccacgcctga gtatggaata ccctattcga ttatattcat
     9181 cacgcagatt cagtataact tttctccttt caggagaaag atttctgagc tctgtatcaa
     9241 acaataattt tgaagctctt ttttccccta aagtaatttt aacatgagtt tctatcaata
     9301 atcgtaattg ctgaagggga tctccatctt ctttttccag atatctgatt ggattgacaa
     9361 tctcttccat ttcctctttt aaaattgaga acagtatctc ttctttactt tcaaaaaaat
     9421 tataaatatt ggcaggtctg aaaccacatt catttgcaat atccttcata ctggtttcag
     9481 catagccttt ttcccagaaa agtacctgtg atttttgcat tatcaatgtc ttctgtttgc
     9541 tgttctcttt tattacaaca tctggcatcg gtattatctc ctcaactaat aaaaaattgc
     9601 tttccctcta ctttcatagt aatgcattat caattaaatc aatcagccac atcataatac
     9661 tatattcttt gaatttacca cattataaca ccataatcgt aaatacaaga aaaaaaacct
     9721 cgtaccattt atacgaggtt ttttataaaa agcaggaggt tccttatagc tttattttta
     9781 ttcaccctta aattctggtt ttcgcttttg tgcaaacgcc atcatgcctt ccatacaatc
     9841 ctttgtcgta aatagttttg ccagttcttc tcgttccatc ttcaaatgca tttcaggcga
     9901 cattgatgga cctgctgaca ataccttcaa aattgcttta acagataatg gtggcctggc
     9961 tgctaattct tctgcaaatt tcaatacttc acccataaaa tcctgttctt caaaaacttt
    10021 atgtacaaga cctatcgcaa gtgcttcatc tgcctcaagt tgttttccaa aaagcattat
    10081 ttctaatgcc tttgctcttc caaccaaacg aggtagccgt aatgtaccgc catagccagg
    10141 cataattcct aaatttgtct cggttaaacc aatccgtgca ccctttttca ttagccgtat
    10201 atgacaggcc attgccaatt cgcatccacc accaagagca tgaccattta atgcagctat
    10261 gacgggttta ggataatctt ctattttatt ccacacatcc tgcccccgct ttaaaaagtc
    10321 aacggcactg aaatctccaa acccctgtga aagatccgca ccagcacaga aaatcttatc
    10381 tcctgatcca gttataacca gcacacggat atttttatct atctccagcg tatctaacaa
    10441 tttccgcatt gtttcaaaaa catcatgact cagctgatta gctttaggtt tgtttatagt
    10501 taaaatacca accatccctt tttgttccag taatactgca tctgacatat taaccctcct
    10561 atctccctat tgttttaaat gaaccatcta taatttcagg ttctggtttc agaatgtcta
    10621 atttaaagcg ctgtgccaga taattaaggc aatcagttaa ttgttgtggt tgaactcctg
    10681 atgccagagc aaacggcccc gcaaatgcgt tcataccata ttttactcca tcatctatat
    10741 cctgaacact tgcagcaata ccttctttat atacctttac tgcttcatta acctgaactg
    10801 ccatgagatg cagcggggtt atttcttcag tatcaggcat ttcaggaata atagctttgc
    10861 catccttcca ttcatatatg ccttttccac tcttcatgcc aagatgacct ttatccaggc
    10921 atccaagcaa aaattttcct ggtttatatt ggggagatag tgtctgtgca taatattcca
    10981 gtgtatggca gaaaacatcc agcccaacaa aatcagctgt ttcaaaagga gccattttaa
    11041 cacccatttt cttcattttc ccatctattt gtgcaggatg gattttgcct tcatctaaaa
    11101 ttgcactgag taatgcctgg ttaggtgcac tgattctgtt aacaataaaa ccaggtctat
    11161 ctttcaaaac ttttacgggg aatttatcaa ttttctttgt taattcaaac aaaatattta
    11221 tagcattttc cgatgttttt gcgccataaa ttaactccac caatttcatt cttgttacag
    11281 ggttgaaaaa atgcatccca gcaaatcgct caggatttgt aacagcagta gctatttcag
    11341 taatgctcat tgttgatgtg tttgttgcca gtattgcatc tgaagcacat gcagatgaag
    11401 cttttgcaaa tacatccttc ttcagttgca tattttcagg gacagcctca atgatcacct
    11461 gagcatctga tactcctttt tgtaaatctg ttgtaacact tattttccca agaatttcat
    11521 caacagtacc ctgcagtttt ccttttgatg caaggaaatc caaactttcc tttactttct
    11581 ttaacgcatt atcaaggaat tcctgcttga tatctatcat ggtaaccatt aaaccattct
    11641 gagcacaaac ctgtgctatc ccatgaccca ttgcccccga acctataact gcaattttct
    11701 ttacatcatt aatactctgc ataattacct ccatttataa aaatcatgtt atttaagttc
    11761 cttacttgac atttccagta catttcgagc aataattaaa tgctgtattt gtccagtccc
    11821 ttcaaaaata tccatgatct tgctatccct catccatttt tctgctagat attgcgttga
    11881 atatcctacc ggcccaagca attcactgca tcgctgggtt atcaatgttg catacctacc
    11941 cgccttagct tttgccattg atgcttctaa attattaaac tggttattat cagccatcca
    12001 ggcagcacgc caggttaaaa gtctcatagc ttcaacattg gcatccatca aataccactc
    12061 acgctcaact gcagaaacat ttgataaatt cttcttataa tcaaatgcat atcctttctt
    12121 ctcatattgt tcccgggtaa attctacagc agcacgtgca ataccagtgg ccattgaagc
    12181 aaccaatggt ctggtattat caaatgtttt cataacacct ttgaatccct cagtggacat
    12241 tttcacttca gggtcaccca atatattatt atagggaatt ctacaatcgc tgagtacaaa
    12301 tgttccagta tcagaggccc tgatgcctaa tttatgttct aaatgctcca gagtaaaacc
    12361 aggtgttcct ttttccacga caaatgattt tataccagct ttgccagctt ttttatcaac
    12421 tgtcgcccat accacaacca cttcacttct gtctgcgcag gttacaaaaa tcttttctcc
    12481 attgagtatc cagtaatcac catcacgagt agctgttgtt gtgatagctc ctgaatcaga
    12541 acctgcaccc ggctccgtaa ttgccatagc agcccactta ttgccaaatc gttccagctg
    12601 ttcatcattg gcaactgcca aaattgctgc attccctaaa ccagcatttg ggaatgacaa
    12661 taaaagagcc gcatctcccc agcacatttc ctcaatggtc actaccatcc ccaagtttgc
    12721 tcctcgtttg ggcttttttt cttcaccgga agctgaagat tcaccctctt tctttttctt
    12781 gggacgagcc atcaccttta tccctctgaa catatcaagt tctttaggat actcatgctc
    12841 agcttcatca tatttccttg agattggacg cagaacacct tctgccaatt tatgcatatt
    12901 atcctgtaat gtttttatat aatcaggtgt tactaactga atcattgttg ccctcctcac
    12961 actattaaat aattgccatg gcttcacaaa tcgcaatact acggccatta cgatagtatc
    13021 gctcaacagg atgatccctg atatacccat gaccaccaag aatctgaaca ccataatcag
    13081 taagtttcat ggtcatttct ccagcataaa tttttgcaag aaatgactca cggtttgcat
    13141 ccatgcctaa ctcaatagct gatgctgctt tccacgttaa cagtctcatt gcatcaactt
    13201 cataggccat ttcagctatc ataaaagcta ttgattgcct gctggctatg ggctcaccaa
    13261 actgctggcg ttcttttgca tacttctttg agtattcata cgaagcacgt gaaatgcctg
    13321 ttccaatagc agccattgca gttcttgttc tctggattaa ccaattaaat gctgtttcat
    13381 cccctaattt gctaatttct ggaatagtac aattttcaaa agtaacttca aaagttggaa
    13441 gtgcccacca accacaattt ttttctcttt ctccaacaga taatcctttt gcatctttat
    13501 ctactataaa cagatgtgga ttaccttcat aatgtgctat aactatcata tgcttagctt
    13561 catcagcaag aggaacaaag cactttttcc catttaaaac atacgtattt cccttctttt
    13621 cagcttttgt ttcacaattc catacatcaa aacccacact gagttcattc acagccaatg
    13681 ttgctggaac aaaggactcc ttacaaaact caggaaggta tttctccttt tgttctgtgc
    13741 tccctaaatt tacaactgga agtacaaaaa gcgcaggtaa catcgcatac acagcaaaag
    13801 ccatgtcccc aaaagctaat tcctcaagaa caagtgtatg cagcacagga gaacttccca
    13861 taccatatcc accatactct tcaggaatca ttgactggat aatacccaat tcccagaatt
    13921 tttggactgc ctgtttatcc aatagtctgt tttcatccca ttggtgaata gcatctatca
    13981 cattagcctg aacaagctgt cggacagtat cctgcatcat ctgctgttct tcagataatg
    14041 caaaacttat ttcttctacc atacaataac ctccttgaat aaaataagta ctgacatgtc
    14101 agtacttatt ttattattaa aattagtcaa ctatttttta ttaaaaatac cttgtgctat
    14161 aattctagaa gctttatcag atattgtttc taaatcctct attaacatgt tatcatccat
    14221 tgttattgtc agataataat gggctaacct gaagactcct ccagctatta gattagccat
    14281 aaaattaaga tccgcctctt tactaatatt gctgaaattc actgcaattt ttagatccgc
    14341 aataatcata ttaattaatt tattttcaaa atgttgaatt ttttgctcaa aatgatctgg
    14401 caggcccaaa ccagcacgta aaattatatt tgcccggtct ctatcttcaa agaaaaatcg
    14461 aaatgcttca tacaaccttt ttttaagata ctgataggga gatattgtct ccaaatcttc
    14521 tttaattgat agttcattga tatatctctc ccattcaatt aaaaaccttt caagtaacga
    14581 tataaaaaga tgttccttat ttgagaaata ttgataaaca gtcccccgcc caatttttgc
    14641 tgattcaata atatctgaaa tttgtgttgc ataatagccc ttttccgaaa ataacctttt
    14701 tgcatgaagt aatattttat ctcttgtatc atctgacctg gtcattattt cctcctttga
    14761 tttttccatc tcattgacaa agagttataa aaaacaatta aattcttaat aagaaattta
    14821 aaaaaaataa tttttttcat aaattaatct tgacaaaaca tggtaatact gacatgtcag
    14881 tattacaagc aaaattttta tatttgcaaa aatattttac ataatattat ttttaaggag
    14941 gaccactatg gttattgcac caaatacttc aattgatcag tttcttacag aaattatgcc
    15001 aaaagtagca acagaaatgc ttcaaaattc caatgcacca aaagaacttg gtggcactga
    15061 aatcaccatg gttgttgaag ttaatggtaa atcctatgga ttcaaagtta aagacggtaa
    15121 agatattgaa acatgtggtg atatcgccaa tgccatgctg agattaaaaa tatccgaagc
    15181 tgatttaaag aaaatgattg aaaccaataa tctcgatatg ttgttaggca tgcaaaagga
    15241 tctgacaaga gctcgatata acgctctctc ttctttgaaa ggtagtttca ttgctaaatt
    15301 aaaaaatgat gatggttctg aatttaccat tgaagcaata cttaacggtg caacatcacc
    15361 acaatcgatt ttccgcatga caacaaaaga ttcagctgct ttaatgaaaa aggaaacaaa
    15421 tcctgttaat cttttcatgt caggtgcaat gaaaatcgaa ggtgacatga gttttgcaat
    15481 ggcaacccaa cccctattca cttgatagat atgctttaca tctcaaccac atttttatgt
    15541 ggttgagatg tttttttatt gatcacctgg aggttaacaa tggaaacttt accctggtac
    15601 aaagaatata aagttttagg tataccaaaa acatttaaac cttatcctga caaaccagta
    15661 tatgatattc ttgaaatagc tgcaaaaaaa tataaaaaaa atggcctttt acaaaaggat
    15721 tattttatat catatccgca acttctagac tatgtaaaca gattagcttc acgtcttcag
    15781 tcttttggat taattaaaaa tgatattatt gcaacgattt taccaacttc tacagaattt
    15841 ataattgctg attacgcaat ttcaaaagca gggctcatcc atctgccatg tagttccctt
    15901 gaacctgctg aagcccttga acataaattt aaagaaggta aacctaaagc tataatttgt
    15961 accagcaatg atattaaaat actaaccagc ttgattaaaa agaccagtgt ccgagccatt
    16021 attattgcta acacacgtac cagcaattct ttcccaacta tcgatacaga taaaattaaa
    16081 atatcaggtt caacagtata tgatttatac tcattaatcc aaaattcttc aggaaatgtt
    16141 aacccagttc ccatcaatgt tgaaactgat attgaaacat tgatatttac aggtggtaca
    16201 acaggcgtcc ctaaaggttg tatgctaacc catagaaaca tatatgctaa ctgtatacag
    16261 aatcttcatg cattaggtgc aggaggattg acattcaagg gagcaatatc tgttatattg
    16321 ggattaccat ttttccatac ctatggccat tgtcttatgc atacaatgat gctttttggt
    16381 tttaatatca tcattatcaa cgatccacgt gatactaaat caatggttga aaacataaag
    16441 aagcactatc ccgttatgca gataggtgta cctgctcaat tcatgaaatt agctaaagaa
    16501 gatcttagtg gtataggcat tattggtctt tcaggatcag cacctttatc tgagtcaaca
    16561 caggaagaat ttgaaaaacg ttcaaatagc ggcataatgg aaggttatgg tctttcagag
    16621 atgtcccccg tgacacacct taatccttct gttatatatc gtattctggg tggaaggttt
    16681 ggtgtacgtc tggtgtctct gttatcatct attccaggaa acagttactt aaccaatctg
    16741 cttttcagat caataggaac aaaaaatttt ggctggattt ttactaaaat tattgcatat
    16801 cgtatgaaaa aaaccagtca aaagcctgga aagaaatctg tggaaaaaag aagaacaatt
    16861 ggtgttccat ttcctgacac tgaagtacgt tttcttgatt ttgactctgg taatatcctg
    16921 acaattgatg atatgctggc tgggaaaaga gcagagatgc ttttaaaggg gccacagcgt
    16981 atgttaggat actggccaaa acctgatagt ggatgtgatg ctgaaggttt cattcatact
    17041 ggtgacatag tacagataga ctcaaatggt tatttttata tagttgatcg ttccaaagat
    17101 atgattaatg tctcaggttt caaagtatat tcaaaagaaa ttgatgatat tgctggagaa
    17161 catccacata ttgaagcagc tgcaactgtt ggaataccaa atcctgaaaa agaaggcagc
    17221 gaagtagttg ttctttttgt tcaactgtac ccacaataca aaaataaaac aaaagaacag
    17281 gacatattaa aataccttca atcaaaggtt gcaaaatatg cagtacccaa gttcataaaa
    17341 attgttgatg aacttccccg aacagcagtt gaaaaagttg ataagaaaat attacgtcaa
    17401 atggctattg accagtttct aatatcaaaa aagaaagtga aaatcaaaac aaaataataa
    17461 ttagagaaca agagaggtac caatgagaga atgtgtattc attgatggtg tgagaacacc
    17521 aaacggcagg gctcatcgtg aaaaaggctg gttcagacat ttaaggcctg atgagctttt
    17581 aacaccagtg tataatgctc tatttgagcg aaacagcaaa gtggctccag atatggttga
    17641 tgctgttttt gttggttgcg ctaatccatc aggcatgcaa aacgatattg gcaggttagc
    17701 atggttagca tctggattac cagattcagt tcccaccaat actttgacca atcagtgccc
    17761 atcaggcatg tccgccacaa tgcatgctgc acgagcaatt ttgactggcg aagcagatat
    17821 tatcattgct gctggtgtag aagatatgga aaaagtacca atgggtgcca atatggattt
    17881 ccctccacga ttgttaaaat attataatgg ggctgatctt ttaatgggtg caacagctga
    17941 aaaggttgct gaacaatata atatttcccg agaagatatg gaaaacatgg ctgtatggtc
    18001 aaataaaaag gcttgggaag caacaatagc tggaaaattt aaaaaggaga tagttcctat
    18061 aaaagggttg gatgataatg gtaatgaatt tttagtagat agagaccagt gggtgcgtga
    18121 taaagttgac gttgaaaaaa tgaaaacgat gatgtcacca ttcaagccca atggtgttgt
    18181 cacggcagca acatcatcac cactaacaca gggtgcttgt gcattgcttc ttatgagccg
    18241 tgaaaaagca gataaactgg gactgcctta ccggtataaa tattcctatg gtgtattagc
    18301 gggatgtgat cctactgtaa tgggaattgg accaattcct gctataaaga aactcgttgc
    18361 acggactggt ttagatctgg acaaagtagg agcaattgaa ctgaatgaag cttttgcaag
    18421 ccagtcatta gcatgtatac gagaattgaa acttgataat gataatgctc cctttaagcg
    18481 tgtgaatgtt tggggtggtg ctatcgcttt aggtcatcca ttaggtgaat cgggtgcacg
    18541 aataattata actctgttga atgtgctatc aactgaattt ccagatgctc aatatggatt
    18601 agcttcacta tgtggtgctt ttgggaatgc aggagcattg ctggttgaaa aagttaatta
    18661 acattaatat ttttactgaa tctccattcc cgtcgcacag agcggcggga atgaaaactc
    18721 aattttattt gattattcat taacgaataa atagtaatta caaataaatt ttacaatatt
    18781 aattttcaaa ggaggtttgc catgaaaaga cttttaatta tctctttaac cattattttt
    18841 gcaagtattt caatccttgt tttttcacaa caatcaaatt ggaaattagt acatgaaagt
    18901 aaagaagatc aagcttcatt acaaattttt acacgtgata ttcccggatc accttataaa
    18961 gaattcaaag gtgttagctt gtctgatatt ccttttgata ttgctctgaa agttattcag
    19021 gactacaata attatcacaa atggtatggc atgtgtaatg cattatatcc aataaatgtc
    19081 cgcaacccca aagatcttga tatgtatttt atccttgatc ttcctgtcgt taaagatcgt
    19141 gatgttgttg ttaaagttga atgcggctgg gatgagaaaa aaggaactgc ctgggtaaat
    19201 atgacatcaa taaatgatag tccctataaa aatgatagtg gtttagtacg aatgacatct
    19261 ttaaaaggaa gatgggatat aatcaaaaaa gatgagaatc atgtagaagt tatttaccag
    19321 gtacatgctg aattaggcgg atcactacct gcctgggttg tcaatattgc agcaaaagat
    19381 caacctttaa agactttaaa aggtgtttat aaatactgta aaaaaataag agataataaa
    19441 taataattaa tattaataat attaattatt g
//
LOCUS       DAORYL010000058        19094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_2405_length_19094_cov_13.631021, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000058 DAORYL010000000
VERSION     DAORYL010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 19094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19094
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2405_length_19094_cov_13.631021"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..571)
                     /locus_tag="PLV81_10655"
     CDS             complement(38..571)
                     /locus_tag="PLV81_10655"
                     /inference="COORDINATES: protein motif:HMM:NF021231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HOM10598.1"
                     /translation="MKFLKRSPIVAIIVAMALCLGIAVAYALTVGQTVQNVELRDAEN
                     NPTMIPLLGTKLVSIFYNDADAADLNDPLADALKAKNFPKEKYEGMGIANLKDSKAPN
                     FIIRKIIQGKIKKYNSTILTDPDLILAKAWNLGDCNNTSVVIFIGKDMKVKYVHKGAV
                     RGEEINKVVKMVEDLIK"
     gene            731..1390
                     /locus_tag="PLV81_10660"
     CDS             731..1390
                     /locus_tag="PLV81_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10599.1"
                     /translation="MFDIYIHRICNSITLQQLICNNNYAYFTSYLNHSSEDQPTALYP
                     RFINISLKSGYAQYIFPFHHLIISYEHFRRHDGNQYRGQPENFHAIELSMINHSLLSM
                     VFNNPESSEFDNIYNNINYGISIAYIFSQQHYPYSFIAKPTVYYYFACCNAGRPFVGI
                     EFLYFMGKENNYEYNIESGLVLNTTIPLTTYYSLKRDIDIDFTGGAHDLYHVIGIRLK
                     L"
     gene            1519..1989
                     /gene="bfr"
                     /locus_tag="PLV81_10665"
     CDS             1519..1989
                     /gene="bfr"
                     /locus_tag="PLV81_10665"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HOM10600.1"
                     /translation="MKGDPKLIETLNSLLADELTAINQYMVHSEMCDNWGYEKLHKHF
                     EKRAIDEMKHAEKLIGRILFLEGLPIVTNLRKIFIGADVPKQLENDRKAEEDAIKAYN
                     DAIVLAGEVKDFATREVLESILKDEDRHIDEIEELQDQINQMSIQIFLTTQVKE"
     gene            complement(2156..2728)
                     /locus_tag="PLV81_10670"
     CDS             complement(2156..2728)
                     /locus_tag="PLV81_10670"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOM10601.1"
                     /translation="MAKVTWKPGTMLYPVPAVLVTSHYNGIDNVCTVSWAGTVCTEPP
                     MISISLRPERYSFQLIQRSKEFVVNIPDKKLVKAVDFCGVRSGKDIDKFAIAHLNKLP
                     AKKVKAPLIEQCPVSIECKVTKIIQLGSHYQFIGKVVVVHVDNTLLDKRGALHLERAG
                     LLCYNHGYYCVVDKQIGKFGFSVKKRKKKK"
     gene            complement(2739..4118)
                     /gene="mgtE"
                     /locus_tag="PLV81_10675"
     CDS             complement(2739..4118)
                     /gene="mgtE"
                     /locus_tag="PLV81_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOM10602.1"
                     /translation="MIAPLIQPEIKELIEKREFSVLKEVFSEWAPADLAELISELPED
                     EQVIIFRLLPKQLQAETFEYLDFEVQSHLLGNLAKKQIADILNDMSPDDRTALFEELP
                     ADILRQMLALMSREERITAMSLLGYPENSVGRLMTTDYIAVKENMTIAQVLHYIRKYG
                     KDSETLNMIYVIDDEGKLIDDIKMRQVLLSQPTRRIKTLMDHKFVSLNAFDDQETAVQ
                     VFKKYDYYALPVVDNDGILLGIVTIDDVLDVEEEEVTEDIHKMAAVEVLEEPYIEEPL
                     LRLTRKRASWLVFLFLGEMLTATAMGYFEEEIARAVVLALFVPLIISSGGNSGSQAST
                     LVIRAMAIGEIKLRDWWRVLIREIQSGFMLGSILAFIGFLRISIWQKLFGMYGEHWLK
                     VALSVSFSLIGVVMWGTLIGSMLPFILRRLGLDPATSSAPFVATLVDVSGLIIYFNIA
                     YLILKGTLL"
     gene            complement(5095..5973)
                     /locus_tag="PLV81_10680"
     CDS             complement(5095..5973)
                     /locus_tag="PLV81_10680"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HOM10603.1"
                     /translation="MQGLIIAIAILVVILVYKGVRIVPQAENWLVERFGKYAATLTPG
                     LNLINPLFSRVAAKIDIREQVLDLPPQGIITEDNATVKVDGVVFYKILDPYKAFYGIE
                     NLQMAISNLALTTLRSIMGKMTLDQSLSSRDKINAELLTVLDQATDSWGTKITRVEIK
                     DIVPPQDLQQAMALQMKAERERRATVLEAEAQKEAQERKAEGYKRAQILEAEARKEAA
                     QRDAEARERLAQAEANAIEYVAKSLKSTGGDPLLYLLGMEYVKGLIKLGESQSSHVVI
                     LPADLIENLKSLFKLK"
     gene            complement(5986..6432)
                     /locus_tag="PLV81_10685"
     CDS             complement(5986..6432)
                     /locus_tag="PLV81_10685"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOM10604.1"
                     /translation="MALSPIAWLAIGIVLMAVEIIMPGFIIFWFGIGAVITASLVYIG
                     LLESEISQWFCFFISSGAFLVFWFGYLKDRVKVKTADDAFDVTLAGKKGKCIKDIIPP
                     QIGEVELFEPFHGVTVWKAQSKELIRKDEQIVVEGADGIKLIVKKL"
     gene            6567..6989
                     /locus_tag="PLV81_10690"
     CDS             6567..6989
                     /locus_tag="PLV81_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10605.1"
                     /translation="MKKYSLLFLSLCVIVSLISCGSSTGKYSDVRALMQDSITAQENL
                     IKDIDAANNAGDVAKAINSYADAIETLQPKIKKLQAKYPELNNENAQLPKELLDLEQK
                     QAEISQKLTTSMMEKMPKYATDKEVMKASMRLAKISQM"
     gene            complement(7067..7561)
                     /locus_tag="PLV81_10695"
     CDS             complement(7067..7561)
                     /locus_tag="PLV81_10695"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF016040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050385 - ureidoglycolate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HOM10606.1"
                     /translation="MKIINPVDLTPENFSPYGYFSKMQNPGTIYFGTSPVKFYRDIIQ
                     LPVISMTNISFSICQVEKRDFVIQKIECHSYCYEGILPLDGDVLIHVAHATQNNVYPY
                     DDIRVFLVPPYTMVVLKAGVWHHAPFAVNSTVNTLIILPERTYANDCIRMDIPEEFQL
                     KILV"
     gene            complement(7650..9635)
                     /locus_tag="PLV81_10700"
     CDS             complement(7650..9635)
                     /locus_tag="PLV81_10700"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOM10607.1"
                     /translation="MNILLNILIIFSISIAVIYVCNRLKIPTIIGLLITGIVVGLYGL
                     QIIKSTTDVELLAEIGIITLLFTIGLELSFDEIASLKRAIAVGVLQVSITILIVTGIM
                     LVWTSFSQAIFFGFIVSLSSTAVVLKVIQELALSESPHGRISLAILIVQDIAIVPLML
                     FIPLLAGVSKNSIPIEMFKLIVKGALIVVVVIVLARYVVPRLLFEIAKTKSRELFVIT
                     IVTICLGVTLATYSMGMSVELGAFLAGIIIAASEYSGQTFSSMMPFRDLFTSFFFISI
                     GMLLNPVIVVSNIVTIANFSVLILSIKIIIIATIVLALGYPLRIAFLTGFALPQIGEF
                     SFILAQIGLSYALLSQEEYQLFLSITIFAMGITPYLIKYSPALVDMLLQLPFPEKLQK
                     GFSYSADEKAHTLQDHIIIVGYGINGRNVAAAAKSIKIPYIIVEMNPETVRKEKKEGE
                     NIIYGDASQEDLLHYLNISSARVLVIAIADRSAIKRIVVLARRINPHIHIIVRARFVG
                     DMKELIELGANEVIPEEYETSIEIFARVLHYYGVPRSDIEAMIESIRAKGYAMLRKLH
                     VDVYKDLPIAEIDMQAIRVCSNSTVAGKTIGQMQFRKVHKFSVVAIKRNDTIITNPGA
                     DDKIESDDILYVVGKREDISKAVKDLRLSDNATSCEL"
     gene            complement(9694..10161)
                     /locus_tag="PLV81_10705"
     CDS             complement(9694..10161)
                     /locus_tag="PLV81_10705"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOM10608.1"
                     /translation="MYDIREATLSDIDDIVTIRMKMLFELGKLETEDGTRKLRSLTRD
                     FFIRKFQTGEFKVFCALKDETIIATTGIQFLERPPLYENPHGIEAYIMNVYTEPEYRG
                     KGVASKLLEKVIEHAKERKAGRILLHAIGKDKRIYEKAGFVSTTDEMQLVLQY"
     gene            complement(10467..10877)
                     /locus_tag="PLV81_10710"
     CDS             complement(10467..10877)
                     /locus_tag="PLV81_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10609.1"
                     /translation="MNDLLYKSFMTVVLSTWNHVVRKPYHVAAFMAVWYYIELLYMMN
                     IAIFFYPPMLISLIGIILGIALSIHILKLYIGNTINVTIHVFVMDVHIAYSAGLTIAA
                     VLSGATWYAVLIVIVRDIIAVIELLLVYTMTKEE"
     gene            10978..11997
                     /locus_tag="PLV81_10715"
     CDS             10978..11997
                     /locus_tag="PLV81_10715"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOM10610.1"
                     /translation="MPCLHYLYSPKVFQGFFMVNENNITLTVPEEYAGTRIDVFIYNA
                     LEEELSRSFIQKLLKNNHITVDGMPVKPNYKVKSDQQIHIIIPQPEKSTIEPEDIPLH
                     ILYEDNDIAIIHKPAGMVVHPGAGNSQHTLVNALLYHFKGLSSIGGVERPGIVHRLDK
                     DTEGIMVIAKNDTSHNVLTKSFQSRNVIKKYEAIVTGKPSTPTDTINKAIERHPKYGH
                     KMTVRDDGKEAITHYTLREVWHTPQGVFSHLSIQIFTGRTHQIRVHLSSIGLPIVGDK
                     LYSKKWEKYNVPFMMLASTYLAFEHPVTGKHLQFSIDLPQHMKDFIKKINTMAFEHIK
                     TEYYD"
     gene            11990..12418
                     /gene="tsaE"
                     /locus_tag="PLV81_10720"
     CDS             11990..12418
                     /gene="tsaE"
                     /locus_tag="PLV81_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOM10611.1"
                     /translation="MTKTTYITQSQEETLALGIELGKHAKPGDIFALIGNLGTGKTIL
                     AKGIAKGLGIEVEITSPTFTLLEVYESTLPLYHFDLYRISDDAELENLFFEEYWFGDG
                     VSVIEWADRALKRLSQDIYIITLEYIDAKRRNITIEHPSS"
     gene            12399..13097
                     /gene="tsaB"
                     /locus_tag="PLV81_10725"
     CDS             12399..13097
                     /gene="tsaB"
                     /locus_tag="PLV81_10725"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOM10612.1"
                     /translation="MNILLLDTATNIEIVALRYNGVVSDKTIQTHHSHSVTLFDTIDS
                     ACKELSTTIHSIQCIGVGIGPGSFTGIRIAVTTARMLAQILQIPLVGIPTQLLFASAI
                     VHSNAHCIVAFDAKKGRVFAARYHITNNSIPQEIVPPGDYTVEQLFNVQNNYDIIAVG
                     DGSIKYQEQMKQLYNTIQFVPDFIPQADRMIHLVESLYAQNPSNYADYRKVLPYYSRK
                     SDAEIMKELREKED"
     gene            complement(13117..>13312)
                     /locus_tag="PLV81_10730"
     CDS             complement(13117..>13312)
                     /locus_tag="PLV81_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10613.1"
                     /translation="QASKKEREEILFFPCLRKERCSQASKKERKEILFFPCLHKEIYI
                     FVSKKERVNIIVYLLKKKYY"
     assembly_gap    13313..13412
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13640..14821)
                     /locus_tag="PLV81_10735"
     CDS             complement(13640..14821)
                     /locus_tag="PLV81_10735"
                     /inference="COORDINATES: protein motif:HMM:NF019245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1577 domain-containing protein"
                     /protein_id="HOM10614.1"
                     /translation="MKDIKQRKQRTFEGLSTLDDIMELLKDEQFQKRLGIKFTLEKST
                     VEINEFIDERTIMLVTDPDYVPVDNKIILYGLVDRYIEIDCDVIEVTGPGYFKCKVVS
                     ARKAAHGRRDLRFKMSPEKVVATNFRVSKHTIDIRNYSIPTGIKVIIEQFENQISKNA
                     DIVKVDILDDRDTLLAYIKKTRSTLYIEDLNNPATFTPINDAFIDIKEVLQEQTPQYI
                     KKLTDSGYKSIIISPVIYIEDDERLIPFAFIQYISKDKTLSMDKVLEIQDLAFKLVDR
                     IRDANTLMIAVHQEILDISRGGAKLKITDNNLKKYILKSKGFIFDIVFKLQAPITIYG
                     DIRYTYIDSEDNLIIGVDFAGNSSRKDEMKRFYSIIQPMEVEYRNRLIKEMRQKKKEQ
                     G"
     gene            complement(14986..15177)
                     /locus_tag="PLV81_10740"
     CDS             complement(14986..15177)
                     /locus_tag="PLV81_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10615.1"
                     /translation="MEQKKSLKQLQADEARKKDKVKKLTAELNAAKKELGALAKAIAA
                     AKKAEANKKKAAKKPAKKK"
     gene            15644..16948
                     /gene="der"
                     /locus_tag="PLV81_10745"
     CDS             15644..16948
                     /gene="der"
                     /locus_tag="PLV81_10745"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOM10616.1"
                     /translation="MKELPVVSIVGRQNVGKSTLFNTLIKQRKAIVDEHPGLTRDVVS
                     YILNYREVTFIINDTPGLDLEPNATLSDDIKQVAFHHLQSTDVFVLLFEKPDIASYDY
                     ELLELIRKFNKPYIIAINKIDYKEDMECLPNFYELGQEFIPISALHKRNIDLLLDTIV
                     THLPHKKKKTIEIDCKIALVGRPNSGKSTLLNAFLGYQRAIVSEIPGTTRDSIDETFK
                     FHGKTIMIIDTAGIRRESRIDNTIEYYALVRSKEAIDKADVVIHLIDATSGITETDKK
                     IADIIMESHKPTVLAINKWDLIEKDHKTFENFKDRILFKYYRAADFPIISISALQKLR
                     IHKLLHTALEINTRAKQKVETSKINVILENVQKKHVMPLLGGDLKIYYATQTNTSPPV
                     IRLFVNKPELFRKDVSRFLEKTLQQALNWHGIPIVFEVSGRK"
     gene            complement(16950..>17477)
                     /gene="recR"
                     /locus_tag="PLV81_10750"
     CDS             complement(16950..>17477)
                     /gene="recR"
                     /locus_tag="PLV81_10750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOM10617.1"
                     /translation="KSASRIAFFLLKTTKANVDALIGAIHQLKDNIRYCEECGGISDE
                     AVCSVCSDNERDKTTLCVVEEPEDMLSIEKSGAFNGLYHVLGGVISPLDGVGPEELSI
                     NKLVERCKRGIHEVIIATNPTIEGDATALYIASLLKPLNIKVMRIAHGLPVGSDLEFV
                     DAPTLAKSITGRREM"
     assembly_gap    17478..17577
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17578..18980)
                     /gene="dnaX"
                     /locus_tag="PLV81_10755"
     CDS             complement(<17578..18980)
                     /gene="dnaX"
                     /locus_tag="PLV81_10755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOM10618.1"
                     /translation="MSYQVIARKWRPQTYSEVVAQEHVAKTIANSISKGRISHAYLFA
                     GPRGVGKTTMARIVAKSLNCVNGPTPEPCGVCEFCTEIKNGNSFDVIEIDGASNTGVD
                     NIRELRENVHSAPLKSRYKVYIIDEVHMLSKSAFNALLKTLEEPPAHVVFIFATTEIH
                     QIPETILSRCQKFIFKRIPVAQIVQQLSTIAEKEGYSIDHKALYHIARASGGSMRDAQ
                     SLLDQVISFGGKEVTEQDVLLLLGIVSLKQYCDCIEIIVQGDIPSLIHFIEGIITAGV
                     DIARFVTGFADVVYAMRLCSVTDVSALLGYSDEELRLLKQTAQHFHDEQLSLFYTMLL
                     DCQKELKYTGNERAVVEMFCIDIMHMLKRPTLASILKQLDESPQAVQKKNLNDITTKE
                     SKTQDDTKIIMHAWTQLLQSFRESKQYMFFKLTHVKASYSEGQLVLSVVGDSSNGYYK
                     QLLSKQEIKQIEDELFKL"
     gene            complement(19008..19092)
                     /locus_tag="PLV81_10760"
     tRNA            complement(19008..19092)
                     /locus_tag="PLV81_10760"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19056..19058),aa:Ser,seq:gga)
BASE COUNT         6237 a         3700 c         3014 g         5943 t
ORIGIN      
        1 acatcccttt ttttgactta atacaatata gtacaaacta tttgattaaa tcctcaacca
       61 tctttacaac tttgttaatt tcttcaccac gtactgcccc tttgtggaca tatttaacct
      121 tcatatcttt gccaatgaag attacaacag atgtattatt gcaatcacca agattccatg
      181 cttttgccag gataagatcg ggatctgtta atattgttga attgtacttt ttgattttac
      241 cctgtattat ttttcttatt ataaagttag gtgctttgga atcttttaaa tttgctattc
      301 ccataccttc atacttttct tttgggaaat tctttgcctt caatgcatca gccagtggat
      361 cattaagatc agcagcgtca gcatcattat agaaaattga aactaatttt gttcccaata
      421 acggtatcat ggtagggttg ttttcagcat ctctcagttc aacattctga actgtctgtc
      481 caactgttaa tgcatacgca accgcaatcc ctaaacatag tgccattgcc actataatag
      541 caactattgg cgaccttttt aaaaacttca tagaaaatcc cccttctgtt ttgttaaaat
      601 gatattatcg ttttctactt aatagaaaaa aagttttatg tcaagtgaaa tatatttaaa
      661 aaaattattt tctttgattg ctttctttat ataatgtact gtattttttg ctcactatcc
      721 tatcattaat gtgtttgata tttatataca tcgaatttgc aattcaataa cattacaaca
      781 actgatttgt aataacaatt atgcatattt cacttcttat cttaatcata gctcagaaga
      841 tcaaccaacc gcactgtatc cacgatttat taatatttct cttaaatcag gatatgcaca
      901 gtatattttc cccttccacc atttaattat atcgtatgaa cacttccgcc gccatgatgg
      961 caatcaatac agagggcaac ctgaaaattt ccatgcaatt gaattatcaa tgataaatca
     1021 ttcgttattg agcatggttt ttaacaaccc ggaatcttct gaatttgata acatctacaa
     1081 taatataaat tatggaataa gcattgcata tattttttca cagcaacatt atccttattc
     1141 atttattgca aaacctactg tttattatta ctttgcgtgt tgtaatgcag gcagaccttt
     1201 tgtaggcatt gaattcctat actttatggg aaaagaaaac aactatgagt ataacattga
     1261 atccggctta gtcttgaata caactattcc tttaaccaca tactattcac tcaaacgcga
     1321 tattgatata gactttaccg gtggagcgca tgacctgtat catgttattg gaataagatt
     1381 aaaattgtaa tttttatatt taattcaagg attaatgctt gattttttta tttaaaaatt
     1441 attttatata aaaaacacca ctaccaggag tagtgatgtt aagctcacat aatataaatt
     1501 tactttaagg agatagccat gaaaggggat ccaaaattaa ttgagacatt gaattcacta
     1561 ctggctgatg aactaacagc aatcaatcaa tacatggtgc attcagaaat gtgcgacaac
     1621 tggggttatg aaaagcttca caagcacttt gaaaaaagag ccattgatga aatgaagcat
     1681 gctgaaaaac tcattggcag aatattattt ttagaagggt taccgatagt aactaactta
     1741 agaaaaatct ttattggcgc agatgtacca aagcaacttg aaaatgatcg caaagctgaa
     1801 gaagatgcaa ttaaagcata taatgatgca attgtattag ctggtgaagt caaagatttt
     1861 gcaactcgcg aagtacttga aagcatatta aaagacgaag atagacatat agacgaaata
     1921 gaagaattgc aggatcagat caatcagatg agcattcaga tatttttaac aacacaggtt
     1981 aaggaatagt attgtttaaa aagggggggt cccaaaagtc gtttcgatac aaatttcgca
     2041 acgctcattg agtaattttg ccagattttg tggcaaaatt gtatcgaaat gagcgagtat
     2101 tacgatataa tgtttgcaaa atttactcaa cgaccttttt tggaacagcc cctttttatt
     2161 ttttcttctt acgttttttt accgaaaacc caaatttccc aatctgttta tccacaacac
     2221 aataataccc gtgattataa cacaaaagcc ctgctcgttc taaatgcaat gcacctcttt
     2281 tatcaagcag agtattgtca acatgtacca caacaacttt tccaatgaat tgataatgac
     2341 ttcctaattg aataattttt gtcaccttgc attcaattga tacagggcat tgttctatta
     2401 atggtgcttt tactttttta gctggcagtt tattaagatg agctatcgca aacttatcaa
     2461 tatctttccc tgaacgaaca ccgcaaaagt caactgcctt tacaagcttt ttatcaggaa
     2521 tatttactac aaattctttt gatcgctgta ttaattggaa cgaatatcgt tctgggcgaa
     2581 gggatatact tatcattggg ggttcagtac atactgtacc tgcccatgat acagtacaaa
     2641 cattatcaat gccgttataa tgtgatgtca ccagaactgc tggtaccggg tacagcattg
     2701 ttccgggttt ccatgtaact tttgccatta tgatatcctt atagcaatgt gccttttaat
     2761 attaaatatg caatgttgaa ataaattatt aatcctgata cgtcaaccag tgttgctaca
     2821 aatggagcag atgatgttgc agggtcaagg cctaatctac gtaaaataaa tggtaacatt
     2881 gaacctataa gcgttcccca catgacaaca ccaatgagag aaaatgatac tgacaaagct
     2941 accttcagcc agtgttcacc atacattcca aaaagttttt gccagatact gatacgcaaa
     3001 aaaccaatga atgcaagaat tgaaccaagc ataaatcctg attgaatttc ccgtattaaa
     3061 actcgccacc aatcacgaag tttaatctca cctattgcca tggcacgaat taccagtgtg
     3121 gatgcctgtg atcctgaatt cccaccactt gaaataatga gtggaacaaa taatgccagc
     3181 accactgcac gtgcaatttc ttcttcaaaa tatcccattg ctgttgcggt cagcatctca
     3241 ccaaggaaaa gaaataccag ccatgatgca cgttttcttg ttaaccgtaa caatggctct
     3301 tcaatgtagg gctcttctaa aacttcaact gcagccatct tatgaatatc ttcggttacc
     3361 tcttcttctt caacgtccag tacgtcgtca attgttacaa tacccaaaag aattccatca
     3421 ttgtccacaa caggtaaagc ataataatcg tattttttaa acacctgtac tgcagtttcc
     3481 tgatcatcaa acgcattgag actcacaaat ttatggtcca tcaatgtttt tattcgcctt
     3541 gttggttgtg ataaaagcac ctgcctcatt tttatatcat caataagttt accttcatca
     3601 tctattacat agatcatgtt caatgtttca ctgtccttgc catatttacg gatataatgc
     3661 aaaacctggg cgatagtcat attttctttg acggcaatat aatccgtggt catcaaacgg
     3721 ccaacactgt tttccgggta cccaaggagg ctcatagccg taatgcgctc ttccctggac
     3781 atcaatgcta acatttgtcg caggatatca gctgggagtt cttcaaacag tgcggttctg
     3841 tcgtcaggcg acatgtcatt caaaatatca gctatctgct ttttggccag attacccaga
     3901 agatgtgatt gaacctcaaa atctaaatac tcaaaggttt cagcttgaag ttgttttggc
     3961 aataacctga agataattac ctgctcatct tcaggcaatt cactgataag ctcagccaaa
     4021 tcggcaggtg cccattcgct gaacacttct tttaacacac tgaactctct tttttcaatc
     4081 agttctttta tttcaggctg aataagaggt gcaatcatca taacatgcct ctgatagtat
     4141 atcaatatat tgtatatggt aatgcttata tcttaacaaa tgacaacaaa tcttatgaaa
     4201 acaattgtat cagttctgaa ttatttctca aataaaataa aacatacagt tacatagtat
     4261 ggttatttat ttacccttat tttaccaata acatacaaat gtcagcaaag caattccttt
     4321 ttataccaac aataattttt atcaatatta ttcctttaaa ttggtgtatc acattctgct
     4381 tatttttacc tattattttt taattaatat ttattgactt gttaataatt ttattatacg
     4441 gtaagcccca taatttattt atagtaaaat attttaatat aaaaggagat acatatcatg
     4501 aaaaactatt agctgtttta tttattgcct tttcaattgt gatttccggt gtttatacag
     4561 aatctatggc aaaaaaagaa acatcggcta agaaaaccga agtcaaggaa aaaaaggaaa
     4621 agaaacaaaa gcaggaaaag taagttaaaa aagaagaaaa gtccgaaaag aaaacaacaa
     4681 aggaaataaa agataagcaa agtaagaaaa aagattctga agaaaaagaa aaaccaaaag
     4741 caaaagataa acaaaaatac aaagataaag ataaaaaaga aaaaaaatca gaagaaaaaa
     4801 caaaacataa aaaaatagaa gataaagata aagataaaga aataaaaaaa gaaaagaaag
     4861 aaaaaagttt tacctacagt ggaaaagaag ataaatggtg aggtaagcct ataggaaaag
     4921 acagtaaagg aagaaccata tatgaaggcc ctagaggtgg acactactac ataaatgaaa
     4981 atggaaacag gacatatatt aaaaaagata aagaataata tataaaagat acccggttgt
     5041 aatgccgggt atcttttttt atttttaatt ctgagttttt atacattaat catactattt
     5101 caatttaaat aaacttttca aattttcaat aagatctgct ggcagaataa ctacatggct
     5161 gctctgtgat tcgcccagtt tgattaaacc tttaacatat tccatgccaa gaagatataa
     5221 taatggatca ccacctgtac tttttaatga ttttgcaaca tattcaatgg cattagcttc
     5281 agcctgggcc aggcgttcac gtgcttcagc atcacgctgg gcagcttctt tgcgtgcctc
     5341 agcttcaagt atctgtgccc ttttatatcc ttctgctttc ctttcttggg cttccttttg
     5401 agcttctgct tccagaactg ttgcacggcg ctcgcgctct gctttcattt gcaatgccat
     5461 tgcctgctgc aaatcctgag gcggaacaat atccttgatt tcaacacgcg taattttggt
     5521 gccccatgaa tcagtagcct gatcaaggac tgttaataat tcagcattga ttttgtcgcg
     5581 ggatgacagt gattgatcca aagtcatttt acccattatt gaacgaagtg ttgtcaatgc
     5641 taaattggat atagccatct gcaaattttc aataccgtaa aatgctttat agggatccag
     5701 tatcttataa aagactacac catcaacctt aactgttgca ttatcttcgg taatgattcc
     5761 ctgcggtggt agatcaagta cctgctcacg gatatcaatt tttgcagcta cccgtgaaaa
     5821 gagtggattg ataagattta agccgggagt taatgttgca gcgtacttcc caaaccgctc
     5881 aaccagccag ttttcggcct gaggaactat tctgacacct ttgtagacca gaattaccac
     5941 aagaattgct atggcaatta tcaagccttg cataaaaacc tcctgttata atttttttac
     6001 tataagttta atgccatcag cgccttcaac aactatctgt tcatctttac gaattaattc
     6061 ctttgattgg gctttccaaa ctgtgacacc atgaaatggt tcaaataatt caacctcacc
     6121 aatttgtgga ggaatgatat ccttaataca ctttcctttt ttacctgcca gggtgacatc
     6181 aaacgcatca tcagctgttt taactttcac cctgtctttt aaataaccaa accagaaaac
     6241 taaaaaagca cctgatgaaa taaagaagca aaaccactgc gatatttcag actccaaaag
     6301 cccaatatac acaagtgaag ctgtaataac tgcccctatc ccaaaccaga atattataaa
     6361 acccggcatt atgatttcaa ctgccattaa cacaatgcct atagctaacc aggcaatagg
     6421 tgataaagcc atattgttac cctttatatg atttttttat ttcttatatt ctttaattaa
     6481 acttatacta aagtcaataa taaaattatt attgaacata tttactatca tgatatactg
     6541 ctatccatac taattaagga ggttttatga aaaaatattc tttattattc ctatcattat
     6601 gtgtgatagt ttctcttatt agctgtggtt catcaactgg taaatacagt gatgttcgtg
     6661 cccttatgca ggattcaatt actgcacagg aaaatttaat caaagatatt gatgccgcta
     6721 acaatgctgg tgatgttgca aaagctatta attcctatgc tgatgcaata gaaacattac
     6781 agccaaagat taaaaagttg caggcaaaat atcctgaact aaacaatgaa aatgcacagt
     6841 taccaaaaga attgcttgat cttgaacaaa aacaggcaga aataagtcag aaactcacaa
     6901 ccagcatgat ggagaagatg ccaaaatacg caaccgacaa agaagttatg aaagcttcaa
     6961 tgcgactggc aaaaataagc cagatgtagc aaaacaataa ggcagggatt aacctcctgc
     7021 cttatgttct ttatattgga atagatatat taacagtcac tatcgcctat actaaaatct
     7081 ttaactgaaa ttcttcaggt atatccatac gaatacaatc attggcatac gtgcgttcag
     7141 gaagtattat cagtgtatta acagtggaat tgacagcaaa tggtgcatga tgccatacac
     7201 cagctttcaa tactaccatt gtgtatggag gcaccaaaaa cactctgatg tcatcatagg
     7261 gataaacgtt attctgcgtt gcatgtgcca catgaataag cacatcgcca tccagcggta
     7321 aaatgccttc gtagcagtaa ctgtggcact caattttttg aataacgaaa tcacgctttt
     7381 caacctggca tattgaaaat gatatatttg tcatggaaat aacgggaagt tgaataatgt
     7441 cacgataaaa ttttacaggt gaagtgccaa aatatattgt gccgggattc tgcatctttg
     7501 aaaaatatcc ataaggggaa aaattttccg gagtcagatc tacagggtta attattttca
     7561 taaaagtgtc tccatagtaa tagaattata catatcactt ttttgatact cgcaatcgct
     7621 tttcagaaga ttttcattaa gatacgatat tacaactcac agcttgtggc attatcactc
     7681 aaacgcaaat ccttaactgc tttagatata tcttctcgct ttccaacaac atacagtata
     7741 tcgtcgcttt ctattttgtc atcagcacct ggattggtaa taatagtatc attgcgttta
     7801 attgcaacaa cggaaaactt atgtactttc ctgaattgca tttgtccaat tgttttaccg
     7861 gcaactgttg aattagaaca cacccgtatt gcctgcatgt caatttctgc aataggcaaa
     7921 tctttatata catcaacatg caacttcctg agcattgcat accctttagc acggattgat
     7981 tcaatcattg cttcaatatc actacgtggc acgccataat agtgcaatac cctggcaaag
     8041 atttcaatgg atgtttcata ctcttcaggg atcacctcgt tggcacctaa ttctattaat
     8101 tccttcatat cacccacaaa acgtgcacga acaataatat gtatatgcgg attgatccgc
     8161 cgtgccagta caacaattct ttttattgca cttcggtcgg ctatagctat taccagaact
     8221 ctggctgaag aaatattaag ataatgtaat agatcttcct gggaagcatc accatagata
     8281 atattttcac cctctttttt ttcttttctt actgtttcag gattcatctc aacaataatg
     8341 taaggaatct ttatactttt agcagctgct gcaacattcc tgccattgat gccatatcct
     8401 acaataataa tatggtcttg taaagtatga gctttttcat cagcactata tgaaaaccct
     8461 ttttgtaact tttcagggaa tggcaattgc aagagcatat caaccagtgc tggagaatat
     8521 ttaatcaaat acggtgttat gcccattgca aatatagtta ttgaaagaaa caactggtat
     8581 tcttcctggg acaataatgc gtacgacaac ccaatttgag ctaaaatgaa ggaaaattca
     8641 ccaatctgtg gtaaagcaaa accagttaaa aaagctatcc gtaatgggta tccaagtgca
     8701 agtacaattg ttgctataat aataatcttg atactaagta tcaaaactga aaaattggcg
     8761 atagttacta tattagaaac cacaatgaca ggattaagta acatccctat tgaaataaag
     8821 aaaaaacttg taaataaatc cctgaacggc atcattgaac taaacgtttg gccactgtat
     8881 tcagaagcag caataatgat tcctgccaga aaggccccta attctacaga catccccatt
     8941 gaatatgttg ctaacgtaac acccaggcaa attgttacaa ttgtaataac aaataattca
     9001 cgacttttag ttttagctat ctcaaataac aatctgggga ctacataccg tgcaaggaca
     9061 ataacaacaa caactatcaa agcaccttta acaataagtt tgaacatctc aatgggaatt
     9121 gaattcttag aaacaccagc caataatgga ataaataaca ttaacggtac tattgcaata
     9181 tcctgaacaa taagaattgc cagagatatc ctgccatgtg ggctttcaga taatgccaat
     9241 tcctgaatta cctttaacac taccgctgta ctgctcaatg aaacaataaa tccaaagaaa
     9301 atagcctggg aaaaagaagt ccacaccagc attataccag taactataag aattgttatg
     9361 ctaacctgca atacacctac tgctattgcg cgtttgagtg atgcaatttc atcaaatgat
     9421 aattctaatc ctatagtgaa aagtaaggta ataattccta tctcagctaa tagttcaaca
     9481 tctgtagtgg atttaatgat ttgaagtcca tacagcccaa caacaatacc agtaataagc
     9541 aatcctatga tggtgggaat cttcaagcga ttgcatacat atattactgc aattgatatt
     9601 gaaaatatta ttaatatatt taaaagaatg ttcatacctg atactgtggt aaaattttat
     9661 aattggatat tttactacat agatattttt acgtcagtat tgcagcacta attgcatctc
     9721 atcagttgtt gatacaaaac cagccttttc atagatacgt ttgtcttttc caatagcatg
     9781 aagcaatatt cttcctgcct ttctttcttt agcatgttca ataacttttt ccagcaattt
     9841 tgatgcaacg ccttttcccc tgtactcagg ttcggtataa acattcatta tatatgcttc
     9901 tataccatga ggattttcat ataatggtgg tctttctaaa aactgaatgc cagttgtagc
     9961 aataattgtt tcatccttta atgcacaaaa aactttaaat tcacctgtct gaaatttacg
    10021 tataaagaaa tcccttgtta atgacctcag cttccgggtg ccatcttcag tttcaagctt
    10081 acccaactca aacagcatct tcatacgtat ggtgacaata tcatcaatat cactcagagt
    10141 agcttcgcga atatcgtaca taaaaaacct ccaccattac acaaatctca acataacaac
    10201 cattcaaatg agatttattt ttttcgtcaa atcataaagt atgttacatc cgttgttttt
    10261 cactttcttt tgtcacaaac gagcaacaaa agaaagtgcc aaagaaaaat gcctttttgt
    10321 taatcttact aacttattct attcattgcg ggctacggaa tagcctccat tcaggtcctc
    10381 gcccgcatcc taatttgcca tgcatcggtg catggcaaaa ccttgcacta gcatttttta
    10441 aaaaatcagt tacatattaa cattaatcat tcttctttgg tcattgtata taccagaagc
    10501 agttctataa ctgctataat atcacgtact attactataa gtacagcata ccaggtagcg
    10561 cctgatagta cagctgctat tgtcaaacct gctgaataag ctatgtggac atccatcaca
    10621 aatacatgga ttgtaacatt aatagtattg ccaatatata gcttcagtat atgtatgctt
    10681 aaggcaatgc ccagtataat tccaataagc gaaattaaca ttggcggata aaaaaatatt
    10741 gctatattca tcatatacag gagttctatg tagtaccata cagccataaa agctgctacg
    10801 tgataaggtt tacgtactac atgattccat gtagataaaa caaccgtcat aaatgattta
    10861 tacaatagat cattcacctg tatgcctcat gtaaaaatga tttgactaaa tgaagcaaaa
    10921 cgtttcattt tattgcacca atcgtacact cttccctgct atctggggga ataaacgatg
    10981 ccatgtttgc attatcttta cagtcctaaa gtatttcaag gcttttttat ggtaaatgaa
    11041 aacaatataa cactaacagt ccccgaagaa tatgctggta cccgcattga tgtatttata
    11101 tacaatgcac tggaagagga actatcgcga agctttatac aaaaactttt gaaaaacaat
    11161 catattacag tagacggaat gcccgtaaaa ccaaattaca aggttaaatc agaccaacaa
    11221 atacatatta tcatcccaca gcctgaaaaa tcaactattg aacctgaaga tatcccttta
    11281 catattttgt atgaagacaa tgatattgcc ataatacata aacctgcagg gatggtagtg
    11341 catcctggtg caggcaacag tcagcacacg ctggtgaatg ctttgctata tcactttaag
    11401 ggcctttctt caattggtgg tgttgaaagg ccaggtattg tacacaggct tgataaagac
    11461 acagaaggca tcatggtcat agctaaaaat gatacttctc acaatgtact tacaaaatca
    11521 ttccagagca ggaatgttat caaaaaatat gaggcgatag ttaccggcaa accatccaca
    11581 ccaaccgata caatcaataa agcaatagaa cgccacccca aatatggcca taaaatgact
    11641 gtacgcgatg atggtaaaga agcaataact cactataccc tacgcgaagt atggcacaca
    11701 ccgcaaggcg tattttcaca tttgagcata cagatattta caggaaggac acatcaaata
    11761 cgggttcatt tatcatcgat agggcttccc atcgttggcg acaagctata ttccaaaaaa
    11821 tgggaaaaat ataatgttcc tttcatgatg cttgcttcaa cctatttagc atttgaacat
    11881 cctgttacag gaaagcatct acaattttct attgacttgc cacagcacat gaaggatttt
    11941 ataaaaaaga ttaatacaat ggcatttgaa cacataaaaa cagaatatta tgactaaaac
    12001 tacatacata acacaatcac aggaagaaac attagcatta ggcattgaac tgggtaaaca
    12061 tgcaaaaccc ggcgatatct ttgcacttat tgggaattta ggaacaggaa agactatcct
    12121 tgccaaaggc attgcaaaag gcttaggaat tgaagtagaa atcaccagcc caacatttac
    12181 cttgcttgag gtttatgaat caacactacc cttataccac tttgatcttt accgaatctc
    12241 tgacgatgct gaacttgaaa atcttttttt tgaagaatac tggtttggtg atggtgtttc
    12301 ggtcattgaa tgggccgata gggcattgaa acggctttca caggacatat acatcattac
    12361 actggaatac atagatgcaa aaaggaggaa cattaccatt gaacatcctt cttcttgaca
    12421 cagcaacaaa tattgaaatc gtagcgctga gatacaatgg tgtagtatca gataaaacca
    12481 tacaaacgca ccattcacat tcagttaccc tatttgacac tattgacagt gcatgcaaag
    12541 agttaagcac cacaatacac agcattcagt gtataggtgt tggcatagga ccaggttcat
    12601 tcactggaat cagaatagca gttaccaccg cgcgcatgct ggcacaaatc ttacagatac
    12661 cactggtagg cataccaacc cagttacttt ttgcttcggc gatagttcat agcaatgcac
    12721 attgcatcgt agcatttgat gccaaaaaag gaagggtctt tgccgcacgt taccacataa
    12781 caaataattc catcccacag gagatagtac caccgggtga ctacaccgta gagcagcttt
    12841 ttaatgtcca aaacaattat gatattattg cagttggcga tggcagtata aagtaccagg
    12901 agcaaatgaa gcagttatat aatactatac aatttgtacc tgattttatc cctcaagcag
    12961 atagaatgat acaccttgtg gaatcattat atgctcaaaa tccatctaac tatgccgact
    13021 atcgtaaagt attgccctat tattcacgga aatcagatgc tgaaatcatg aaagaattac
    13081 gagagaaaga agattaaatc tacttttttc tagcatttaa taatattttt ttttcagcaa
    13141 ataaactatt atattaactc tttctttttt agacacaaat atgtatattt ccttatgtaa
    13201 gcaaggaaaa aataatatct cttttctttc tttttttgag gcttgactgc acctttcctt
    13261 gcgcaagcaa ggaaaaaata atatctcttc tctttctttt tttgacgctt gannnnnnnn
    13321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncttgcgca agcaaggaaa aaataatatc
    13441 tcttctcttt ctttttttga cgcttgacta catctttcct tgcgcaagca aggaaaaaat
    13501 aatatctctt ttctttcttt ttttgacgct tgactacatc tttccttgcg taagcaagga
    13561 aaaaataata tctcttttct ttcttttttt gacgcctatc tgcatctttc cttgcgtaag
    13621 caaggaaaaa tattaaatat tacccttgtt ctttcttttt ttgacgcatt tcttttatga
    13681 gcctgtttct gtactctacc tccattggct gaattattga gtaaaaacgc ttcatttcat
    13741 ccttacgaga tgaattacca gcaaaatcta ctccaattat aagattatcc tctgaatcaa
    13801 tataggtata gcgtatatca ccatagatag ttatgggtgc ctgcaattta aaaacaatat
    13861 caaaaatgaa tccttttgat tttaagatat atttcttcaa attattgtca gttatcttta
    13921 gtttagcccc acccctgctt atatccagta tctcctggtg gacagcaatc atcaatgtat
    13981 tggcatcacg tattctatca acaagcttga aggctaaatc ctgaatttcc agcactttat
    14041 ccatcgataa agtcttgtct tttgaaatat actgtataaa tgcaaatggg ataagacgct
    14101 catcatcttc aatatatata acaggggata taattattga cttataacca ctatcagtta
    14161 gcttttttat atactgagga gtttgttcct gtaacacttc ttttatatca ataaaagcat
    14221 cattaatagg agtgaacgtt gcaggattat taagatcttc tatatataat gttgaccttg
    14281 tttttttgat gtatgctaac aacgtatcac gatcatccag tatatcaact ttcactatat
    14341 cagcattttt tgaaatctgg ttttcaaatt gttcaataat aacttttata cctgtaggaa
    14401 tgctataatt acgaatatct atagtatgtt tggaaaccct gaaattggtg gcaacaactt
    14461 tttcaggact cattttaaac cgtaaatcac gccttccatg agcagctttt cgtgcactta
    14521 ccaccttaca tttaaaatac ccgggtcctg ttacctcaat gacatcacaa tctatctcaa
    14581 tatatctgtc aaccagccca taaagaataa ttttattgtc aactggaaca tagtcagggt
    14641 cagttacgag cattattgtg cgttcatcaa tgaattcatt aatttcaact gtagattttt
    14701 caagggtaaa tttaatgccc aatcgtttct ggaattgctc atctttcaac aattccatga
    14761 tatcatctaa tgttgacagc ccttcaaagg ttcgttgttt tctttgcttt atatctttca
    14821 agttcagcct caatacacaa aaaacacctg taagaatctt tatttttctt atcgaaaaat
    14881 tattttaatt acttcactat aaaactgcaa ttactttttt atataaaaaa gaggtggtta
    14941 cccacctctt taaatttaat tctatgatag gcaaatataa ataacttatt tcttctttgc
    15001 tggcttttta gcagcttttt tcttgtttgc ttctgctttc tttgctgctg caatagcttt
    15061 tgccaatgca cctaattctt tctttgctgc attgagctcg gcagtcaatt ttttgacttt
    15121 gtctttcttt cgtgcttcgt cggcctgaag ctgttttaat gactttttct gttccatcga
    15181 ttacaccacc tcgattttaa aatttttcat gcatagtgca tgttaaaaaa tataagcaat
    15241 taatcctaat gatgaacata taacatatat attttaatat tgcaacaaaa aaatgttttt
    15301 gatatatgaa aattgtaaaa attttttttg attaataaat tccatattca cataagatac
    15361 gatgctggga atatatattc caaataactt tttctttttc taaattcgaa taaaatcatt
    15421 aaaaacatta gaaattaatt tcctaattgc ttaaataact attgacacct tgttcttaca
    15481 tatatttctt attacatact gacagcttct gaatcaaata ttaagaacaa aatagtgcaa
    15541 acatactcta tgcagctgaa agcaggagat ataattaaca ttcaatattt acagtattat
    15601 ttttaagata attttgcata aaacaaatag tgtgagggat tctgtgaaag aattaccggt
    15661 tgtttcaatc gttggaaggc aaaatgttgg aaaatcaact ctgtttaata cattaattaa
    15721 gcagcgtaaa gcaatcgttg atgaacatcc cgggcttaca cgcgatgttg tttcatatat
    15781 tcttaactac cgtgaagtta cttttatcat caatgatact ccagggcttg atctggaacc
    15841 caatgctact ctgtctgatg acattaaaca ggtagcattt catcacctgc aatcaaccga
    15901 tgtttttgtt ttgttgtttg aaaaacctga tattgcttca tacgattatg aattgctgga
    15961 attgatccgc aaattcaata agccatatat cattgctata aataaaatag actataaaga
    16021 agacatggag tgccttccaa atttttatga acttgggcag gaatttattc caatttcagc
    16081 attacataag cgcaatattg atcttctcct ggatacaata gtaacgcatc ttccccataa
    16141 aaagaagaaa actattgaaa ttgattgtaa aattgcactt gttgggcgtc caaattcagg
    16201 caaatctaca ctattaaacg catttctggg ctatcagcgt gcaatagtat ctgaaatacc
    16261 aggcacaacg cgcgatagca ttgatgaaac attcaaattt catggcaaaa ccatcatgat
    16321 tatagatact gctggcatac gcagagaaag cagaattgac aatacaattg aatattatgc
    16381 tttggtccgt tcaaaagaag ctatcgacaa agctgatgtt gtcattcatt taattgatgc
    16441 aaccagcggt attaccgaaa cagacaaaaa gatagccgat atcatcatgg aatcgcacaa
    16501 acccacagtc cttgccatca ataaatggga cctgattgaa aaagatcaca aaacatttga
    16561 aaattttaag gatagaatac tatttaaata ctatcgtgca gccgacttcc ctatcatttc
    16621 catatcagcg ttacaaaaat tacgcatcca taaattatta catacagcac tggaaataaa
    16681 cacccgtgca aaacagaaag ttgaaacatc aaagataaat gtgatactgg aaaatgtgca
    16741 gaaaaagcac gttatgcctt tgctcggtgg ggatttaaag atatactacg caacacaaac
    16801 caacacatca ccacctgtaa tacgcctttt tgtcaacaaa cctgaattat tccgcaaaga
    16861 tgtatcgcgt tttctggaaa aaacattgca gcaagctcta aactggcatg gaattcctat
    16921 agtatttgag gtaagtggca gaaaatagcc tacatttccc tcctgccggt aatggatttt
    16981 gcaagcgttg gtgcatcaac aaattccagg tctgaaccaa ctggcaaacc gtgagcaatg
    17041 cgcattactt taatattcaa cggtttcaga agggatgcaa tatacaacgc tgttgcatcg
    17101 ccttctattg tggggttggt ggcaataata acttcatgta taccacgttt acatcgctca
    17161 acaagcttat tgattgaaag ctcttcgggg cctacaccat ccaggggaga aataacacca
    17221 ccaagtacat gatacaggcc attaaatgca ccggattttt caatgcttag catatcttca
    17281 ggctcttcaa ccacacacag ggtggttttg tccctttcat tatcgctgca cacactgcac
    17341 acagcttcat cagatattcc accacattct tcacaatatc tgatattatc ctttaactga
    17401 tgtatagcgc caatcagcgc atcaacattg gcttttgttg tttttaataa aaaaaacgca
    17461 atgcgcgatg cacttttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagt
    17581 ttaaacaatt catcttcaat ttgttttatt tcctgtttcg atagtaactg tttataatat
    17641 ccatttgaac tatcgcccac aaccgaaaga accagttgcc cctcactgta cgacgctttc
    17701 acatgggtaa gtttaaagaa catatactgc ttactttcac gaaagctttg caacaactga
    17761 gtccatgcat gcatgatgat ttttgtatca tcctgagttt tgctttcttt tgtcgtaatg
    17821 tcattcagat tttttttttg aactgcctgc gggctttcat ccagttgttt taaaattgaa
    17881 gctaacgttg gccttttcag catgtgcata atatcaatac agaacatctc aacaacagca
    17941 cgttcattgc ctgtatattt caattctttc tggcaatcca gaagcatagt ataaaaaaga
    18001 ctcaactgtt cgtcatggaa atgctgtgca gtctgtttta atagtcttag ctcttcatca
    18061 gagtagccca gcagtgctga cacatcggta acactgcaca atcgcatagc atacactaca
    18121 tctgcaaaac cggtaacaaa acgggcaata tcaacacctg cagtgatgat accctcaata
    18181 aagtgaataa gtgatggaat atcaccctga acaataattt caatgcaatc gcagtactgt
    18241 tttaaagaaa caatacccaa aagcaaaaga acgtcctgct cggtaacctc tttaccacca
    18301 aatgatataa cctgatccag cagtgactgg gcatcacgca ttgaaccgcc actggcacgg
    18361 gcaatatgat acaatgcctt atggtcaatg ctataccctt ctttctcagc aatggtggaa
    18421 agctgctgta caatctgggc aacagggatg cgcttgaaga taaacttttg acaccgtgac
    18481 agtattgttt caggtatctg gtgtatttcg gttgttgcaa atataaagac tacatgtgct
    18541 ggtggttctt ctagtgtttt gagaagcgca ttgaaagctg attttgacag catgtggact
    18601 tcatcaatga tatatacttt atagcgggat ttcaaaggcg cagaatgtac attttcgcgc
    18661 aattcgcgga tattatcaac accggtattg gatgccccgt caatttcaat aacatcaaat
    18721 gaattgccat tttttatctc ggtacagaat tcgcatactc cacatggttc gggtgttggc
    18781 ccattaacac aattgagtga ctttgccaca atgcgtgcca tggtggtttt gcccactccc
    18841 cgcggcccag caaaaagata agcatgggaa attcttccct tgctgatgct atttgctata
    18901 gtttttgcta catgttcctg ggctacaact tcactgtagg tttgcgggcg ccactttcgt
    18961 gcaattacct gatatgacat atattccttt taccaagctt ttttcttcgg agagaggggg
    19021 attcgaaccc ccggtagctt gcgctacaca cggtttccaa ccgtgctctt tcggcctctc
    19081 agacatctct cctg
//
LOCUS       DAORYL010000059        18470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_2489_length_18470_cov_13.181863, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000059 DAORYL010000000
VERSION     DAORYL010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 18470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18470
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2489_length_18470_cov_13.181863"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..1177
                     /locus_tag="PLV81_10765"
     CDS             47..1177
                     /locus_tag="PLV81_10765"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOM10619.1"
                     /translation="MIPFISIPPLLMAAIAVYVAVSYTLMFARRKNEPEHIWFALMCY
                     TIALYDIFSALLYMSPSPLHSILYQRMQFAVLACFIIAITWFIASLVHINSTLVYTIT
                     LIMGLFIIAGFVFDSSYTLNSDNPYIKQFHFFGTTITYNEADPGLVYVLQYIAMVITG
                     LYLLYKLFIWCRYGEIHIKILFISFSIFLLASINDVCVGKGMYQFIYILEYAYFFVIL
                     SMAYILQSRFIKLHREIEHLTFLFNRKLLEKERYLQPISSESKHKQLSATNSEKIQHA
                     VEYIHANFAFDLSREGLAAMIDLHPDTFSRLFKTYTGKSLPDYINELRINYARELLTS
                     TNDSIVTIAFKTGFESLSTFNRAFHKILNTTPTAYRKHNSKF"
     gene            1319..2917
                     /locus_tag="PLV81_10770"
     CDS             1319..2917
                     /locus_tag="PLV81_10770"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOM10620.1"
                     /translation="MKPTYILTHDVGTTGNKTCLYKVDNKITLIDSYLVEYPLYMLPD
                     GGVEQKADQWWDAICKATKMVMKKSGIPPAKIAAMAFCAQMQGSVFIDKDGNALRNPM
                     SYLDQRATRQIEKYLYKGIVKIEKWNAYKTIKSLIVTGGLAATPKDPLWKYHWVKDNE
                     PDIFAKAYKWLDVKDYLIFRCTGKLGMTQDSANVTFIYDTRKGRLGWHKGLCKIFDVN
                     MEHLPPVFNSTDTIGTITKKAARELGIAEGIPVFGGGGDVPLTLVGSGCTDLYDTNIY
                     VGTSGWVSSNVDKRMVDIGNFIASILGAIPNHYVYVAEQETAGACLKWVRDHLALDEI
                     GMYLQARHIADKSKEYESLYDFLNTVVMQTPPGAGNVLFTPWLHGNRSPREDPYARGM
                     FFNLSLTTGKRQMIRSVLEGVAYHILWMLEAMEKKIPRQEVLRFAGGGAKSDVWCQIM
                     ADITGRNIEAIENEINAGTVGASVVCAVGLGILSSFNEAKRLVGVRKIFKPQPQYTDM
                     YTRNFKVFTKLYEKNKDLFKILNY"
     gene            2935..4392
                     /locus_tag="PLV81_10775"
     CDS             2935..4392
                     /locus_tag="PLV81_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOM10621.1"
                     /translation="MKQEYAISHWPDSKEILEKLRVLANELPVYQINRNKMNEYLEKY
                     ETKFKSSKQLVEKARQRIPGGVQHNLAFNYPFPLAIAKADGAYLYDVDGNKFIDFLQA
                     GGPTVLGSNYKPVRDKVIEVINECSPVTGLFHEYELLLAELIHSHIPSVELFRSLASG
                     TEGCMAAIRAARCFTDKKYIIKVGGAYHGWSDQMVFAMRIPGLGTLDSHGIPEGCYTY
                     MQEFYPNDIEGLEKLLKENESKGGTAAVIVEPLGPESGTRPVHYDFNAKVRELCDKYN
                     SLLIFDEVVTAFRVGLGGAQGYFGVKPDLTVFGKCVAGGYPAAGGVGGRREIMETFAA
                     GIQGLKKRAMVGGTLSANPLSCAAGYFAIKAIEETQAHIKAGKAGDKLTQGLQEIFKR
                     YDLPFVAFNHGSICHMETGAAMLMELTNPKIFEQVGQRRKVMEELGAALMVEGIITLA
                     GSRIYTSMADTDDVIDDALERFDRVFKYFEPVKEK"
     gene            4394..5605
                     /locus_tag="PLV81_10780"
     CDS             4394..5605
                     /locus_tag="PLV81_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOM10622.1"
                     /translation="MTHEIKVYNYRWIILFIFALLNIVIQIHWISFASITSEAAAFYN
                     VTPLSIGFLSMLFMIVYVFISLPASYIIDTHGIKLGIGIGAVLIGIFGIIKGLYAYSF
                     NAIVTTQIALAIAQPFILNAYTRLSAKWFPLDERATAAGIASLAQYIGIIVGLAATPF
                     LVHSYSIPHTMYIYGILSIAIAVLFLLFMKEEPPTPPCEKGQEERFKVKEGLQHIFSM
                     KQMWLVILLFFIGLGMFNAVTTWIEQILEPRGFNSEQAGIVGASMMAGGILGAIILPL
                     LSDHYRKRKVFLTICMAFLIPGLAGLTFAQHFNTILISSFILGFFIMSAGPIGFQYAA
                     EISYPAPESSSQGLLLLSGQISGILFIFAMDAFRTSTGAMTPFMIAFIVLMVINTLLT
                     FKLKESNLISS"
     gene            5743..6396
                     /locus_tag="PLV81_10785"
     CDS             5743..6396
                     /locus_tag="PLV81_10785"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOM10623.1"
                     /translation="MSQYTMYKEQILQWCQWLNEHGFFGTQLGSGGNISCRIENEEKI
                     AVTPSSLPYKDMTTSDICIVDYNQNVIEGKKPTMELSMHLAIYKARDDISAVVHTHQI
                     YASILSVMNIDKPIQAMFDEIAFAIGDEVAFVPYALSGTPELASNVASAVTNKCKCYI
                     IKNHGAIALGKNLEQACLHAEMLEKVSMVYYYSLAAGKEASSLPEPIMQVIRQLFGK"
     gene            6526..6996
                     /locus_tag="PLV81_10790"
     CDS             6526..6996
                     /locus_tag="PLV81_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HOM10624.1"
                     /translation="MKKSVSLLVLFMLVAAISIAEAGVVRNNAGCGVGSMIFGDKDGL
                     LFEILATTTNGICGNQTFGMTSGTLGCAPMKGIVSNEKINLYVADNMDNLAKDIAKGN
                     GEYLETLALLMNVPESEKQQFFTKLQSNFNKIYTSNDVTSTEVVKNIEAVLQNS"
     gene            7070..9007
                     /locus_tag="PLV81_10795"
     CDS             7070..9007
                     /locus_tag="PLV81_10795"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HOM10625.1"
                     /translation="MPFFILLIHFTAILFYHYAYAQQKYPYETIIDIPSSTTISKDLF
                     QSINIDQLISIAHTKNLCNTAQWWALLHYKKNIFGIKSLVDDPKFFLAPDGKYNPQSE
                     LDATIRYLYSYHEKGNPIDHFMARYEWLKKELQTPSPYEVFCKKYQEIVSFKDTIKVN
                     LVFPTGYINSPASMFGHTLLTVQSQYNPGLLSSAINYAANTNETFGPLFAFRGIFGFY
                     KGYFSIMPYYMKVQEYSDLESRDIWEYTLNLNQEEVRFMLFHIWELDNVYSDYYFFDE
                     NCSFNLLYLMEVTRPDSHLTNNFFFWVIPIDTIRKVINAGFVESTSFRPSRTTTINYM
                     VLQLNPDQIKVVKSIANHENKALTNLLNSSFTETQKIQILDCASEFIQYRYANKEYTQ
                     KEYQDLFINVLKIRSSFPQTLSYTIPDPPRPETGHYSSHIRLQGGIFDKKPYSELVYR
                     PSYHDLCDDPSGFIPGSQIIFGETAIRYDYDNQKLLLQRLELINIESLSPINPLIKPL
                     SWKVITGFDRVPFASNNQTLVYQLNTGSGYTIEVFKWLQIYGMIEFSGLLNNGFKNNI
                     EAGPGVSAGFVLQPFQHFRIRGYIVDTYYLYGEACNFLTMKAIMQYNISQRYSMRFEV
                     SSWYNEQWYNQFSAALTFYFW"
     gene            9016..9813
                     /locus_tag="PLV81_10800"
     CDS             9016..9813
                     /locus_tag="PLV81_10800"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOM10626.1"
                     /translation="MNYRFHIILCICLVVTGCLDSVIYHPDTMTPTTSALPFPAEDVL
                     IHLNETVTLHGWFISSQTARFTVCYFHGNAGNIYDRIELLKLLHSIPLSIFIIDYRGY
                     GKSSGKPSEKGLYEDAMAAWEYLTDVKKISPKEIILFGRSLGGPVALYCASRKNPAGI
                     IIDSSFTSIKSMVQYHSSGCMSLFFKERYPAIDYIQKIAIPVLILHSRDDETAPFSMG
                     QELYRQCPSNKKYFVELRGGHNNNFLVDAPLYRNSIKEFVLSLTSEE"
     gene            9821..10375
                     /locus_tag="PLV81_10805"
     CDS             9821..10375
                     /locus_tag="PLV81_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10627.1"
                     /translation="MIKRYVALFVCLLFPLYSEAQDCVSISFNTLRMLAESRIDPCPI
                     CQKKLQRQALGQITKDIFPGKKIMFNSSCSLIKTQQTKDNELMLNTDYDSKIMINGIE
                     TNLPYIILKFYTHENHIVGIDEKDYTSREIQNLYKNLMYNKPVEAEVIVISYKYSDFA
                     SSIYLPERNTLIIHCKVIRLQQIQ"
     gene            complement(10385..11581)
                     /gene="glpC"
                     /locus_tag="PLV81_10810"
     CDS             complement(10385..11581)
                     /gene="glpC"
                     /locus_tag="PLV81_10810"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005298671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HOM10628.1"
                     /translation="MIKLENISFDHCIKCTVCTIYCPVARVTHLYPGPKQSGPDTERL
                     RIKNPQLLDISLKYCSNCKRCEIACPSDVKIADIIQTAKWRYSKKEFRIRDFLLSRTD
                     LMGTFGTIFSPIVNFFTHLGIVKYFLDLIFQIPKGRTFPNYASMTFRKWFKEAKKYQN
                     KYLEKVVYFHGCYVNYNYPQLGKDVVKVLNALGKGVIITKEKCCGVPLIANGYIKKAQ
                     SHGRHNIRVLSRALPNEQTRIVSASSSCSLALKHEYPNLLELDNSPIANNIAYITQYV
                     LEEFEKGNIPNLKPLKYTVAYHSPCHLERMGNVAFTIELLRKIPGLTLKILHSECCGI
                     SGTYGFKNEYYAISQAVGSELFKIIEEAKPDFVVTDCETCKWQIEMNTQYTVIHPISL
                     LAEAIE"
     gene            complement(11586..12881)
                     /gene="glpB"
                     /locus_tag="PLV81_10815"
     CDS             complement(11586..12881)
                     /gene="glpB"
                     /locus_tag="PLV81_10815"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HOM10629.1"
                     /translation="MKFDCIIIGGGISGLTAGIRCVKEGLHTAIISSGMSALHFSSGS
                     IDVLGYINNKVVYKPFEALDTLTGTIAQHPYTKCGKPLIIEALHFFKQECETQNVYLF
                     DNENLNHFHVTALGTLKPTYFSQRSVFNEDVYNRFKSMKKIVIVNFEGYRDFHPQLAA
                     AQLSKHALFANCEIIAKSIDMPQLQDLTRHPHEFRSIDIARIFEDEENVYKIADAIRL
                     AAEGADFVVIPAFMGIHNFNQIHNLLQSRTRLLIYEVPTLPPSILGLRIDNALKSRFA
                     QLGGVYIAGDKVVRAEINNDVVQAVYTANHPENPLEAKFYILSTGSFFSGGMISEFNN
                     IYEPIFKLKLHYEPERNKWYSPQFFYKNSHPFLEYGVVTDENLRPYDSNNNIIQNVYC
                     SGAILAHYNPIREGCGSGVAISTAYKAANQIISLYKLMR"
     gene            complement(12878..14503)
                     /gene="glpA"
                     /locus_tag="PLV81_10820"
     CDS             complement(12878..14503)
                     /gene="glpA"
                     /locus_tag="PLV81_10820"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011898787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HOM10630.1"
                     /translation="MKTLETDVIIIGGGATGCGVLRDCTLRGINAVLIEKNDIASGTT
                     GRNHGLLHSGARYAVKDLESATECINENRILKKIAWHCIEPTGGLFVTLPEDDPTYHN
                     RLLESCKFAGIDAKEISIKKALAIEPNLNPNILSAIWVPDGTIDPFRLASANILDSVE
                     RGAIVFTHTEVERIMLKQNQVAGVQCYDKVTKEYFEVYAPIIVNASGVWGQHLCSTAG
                     IELKMFPSKGSMVIIDYRINNVVVNRCRVPSDGDIIVPGDTVSLIGTTSRKVSYDKID
                     ELVVDDDEIDVLLADGEKLIPNVSKARVLRAYCGVRPLVAVSGEMDGREISRGVVLID
                     HKERDNVQGLITIAGGKLMTYRLMAEMTTDLVAKKIGNTKSCVTHKIPLPGSEKRVGY
                     TKKIKYFSGIPNSVVGSTHYRHGERVHNILSLQKRNYGLICECEMVTLGEVEYAIKNL
                     KVKDLVDLRRRTRIGMGPCQGELCAYRAAGLFTEFGEFTPDTSTKLLKDFLEERFKGM
                     KPVLWGDGLREFEFTYWIYQGLFGLGLVEEEEQ"
     gene            complement(14774..15394)
                     /locus_tag="PLV81_10825"
     CDS             complement(14774..15394)
                     /locus_tag="PLV81_10825"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOM10631.1"
                     /translation="MKAELRRQHILDCAKKVFAEKGYYEAYVEDVIKVAHIGKGTIYE
                     YFRNKEDLFLSLLIRSLEEWKRVVSISHQEIKTLDPVSYFRHRIKQTIVFLNNDRELT
                     SILLKTGFGFNPHIQKLIGEFEKNLRKHILHDLRLAKHLGVLRDGVDLQITTSILIGG
                     LLMIAYQAILSVPRLSEPEIDELTDKFMVTFIRGMFKPEFVNQYIN"
     gene            complement(15481..18360)
                     /locus_tag="PLV81_10830"
     CDS             complement(15481..18360)
                     /locus_tag="PLV81_10830"
                     /inference="COORDINATES: protein motif:HMM:NF019314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOM10632.1"
                     /translation="MLLTLVHNITLLIALSVIWGPILTLRKNYPNTYRIFSGLLFGLS
                     AIAAMSTPLVFEEGIIYDGRSVVIALSGLYGGGIASLIASFIAGSYRIILGGPGVYVG
                     VASVIVSALSGLVVRRIFKNEPTNIPLYGIVLFAVITHVLVLLCQLLLPWPRGITVIA
                     TIGIQYLIVLSSGLCAIALIFKTIENRTLALQNLQASADLLHDVLSVNPSVVFSMDPS
                     TKTITWISPNVEVVLGYKQDEIINTPLNSYIHKDDVYLYDKMFEEPGDNNFISVECRI
                     ITLYQTVVWGDIKIRVIRDEKGRIAALFGSIRDITQRKKVEHELKESEERYRTIFKNS
                     KAVMLIIDPDTGDIVDANEAAVEFYGWSRKELQSMQIQQINTLTPEEIKARMEEARAC
                     KRVYFEFKHRREDGSIRDVDVYSSAITIKGKQYLFSIIHDVTAKRQAEQALKKSEELF
                     RLIFNNAPMGIYAFDENGVITACNDNFVKIIGSSREALIGLNTLKLPDERVVDAVNKC
                     LQGIQSEYNGWYKSVTADKITPARALFSPLIVENKIVGGLAIVEDMTYQLEAEAQKEK
                     LEEQLRQAQKLEAIGRLVGGIAHDFNNILSIISGYAELIKIKIEKDNPIYSDLEEISN
                     ATKRSAEIVRQLLTFSRQQKVNPIRVNPNYIIRSTEKMLAAMVGENIKVQLELADDVY
                     DIWIDTATFVQILTNLASNSRDAIKDVGNIKIVTKNRYLDEEFCTIHPGCKPGDYVMI
                     QFIDDGSGMDKETLERLFEPFFTTKEVGKGTGLGLSTVYGIVKQFNGFINVYSEPGKG
                     TVVNIFFPKYVEQLPEVQLSGHVRDNGRTEDRGIGISKNKILVVEDDLIILDMLKKML
                     LLLDQDVDAFSDPLEMLQVAENYKNNIDILITDIVMPQMNGIELFNRLKLIIPDMKVI
                     FISGYSDDSYRDETKGIKHSVFLQKPFTLDELKNALLKLDQNYEQ"
BASE COUNT         5823 a         3529 c         3221 g         5897 t
ORIGIN      
        1 tcatttattt cacaattatt ttataatgca ttgaaatttt attatcatga taccatttat
       61 ttccattcct cccctactta tggctgctat agcagtgtat gttgcagtat catacactct
      121 catgtttgca cgaagaaaaa atgaacccga acatatatgg tttgcattaa tgtgttatac
      181 aattgcattg tatgatatat tttccgcttt gctgtatatg tcgccatcac cacttcattc
      241 aatactgtat caacgtatgc aatttgctgt attggcatgt ttcattatag caataacttg
      301 gtttattgct tcactggtgc atattaattc tactttagtt tacaccatca cattgataat
      361 ggggttattt atcatagctg gatttgtttt cgacagttca tatacattaa actctgacaa
      421 cccatatata aagcaatttc atttttttgg aacaacaata acgtacaatg aagctgatcc
      481 tggtcttgtg tatgtgttac agtatattgc catggtaata actggtttat atttattata
      541 caaacttttt atatggtgcc gctatggtga aattcacata aaaattctat ttatcagttt
      601 ttcaattttc cttcttgcca gcattaatga cgtatgtgtt ggtaaaggta tgtaccaatt
      661 tatttacata cttgaatatg cctacttttt tgtcattctt tccatggcat acattttaca
      721 atcacgcttc attaagctcc acagggagat agaacatctg acttttttat ttaacagaaa
      781 gcttctggaa aaagaaagat atttacaacc aatatccagt gaaagcaagc ataaacagct
      841 ttctgcaaca aactcagaaa agattcagca tgccgttgag tacattcatg caaactttgc
      901 atttgatcta tctcgagaag ggcttgctgc aatgattgat cttcatcctg atacattcag
      961 caggcttttt aaaacataca caggtaaatc cctccctgac tacataaatg aattgagaat
     1021 taattacgca cgggaattgc ttacatcaac caatgattca atagtaacta tcgcctttaa
     1081 aactggcttt gaaagccttt ccacgtttaa ccgtgcattt cacaaaattc ttaataccac
     1141 tccaacagct taccgtaaac ataatagcaa attctgatta atcagatatt gcgtagacaa
     1201 atacacattt atatcctata aaaaagcatt ctaagatcct tttgcaatgt aatgtagaat
     1261 cgctatgtaa ggcggttaaa aaaataagtt taaaagtatt atttctggag gcatcaaaat
     1321 gaaaccaaca tatatattaa ctcatgacgt gggtaccaca ggcaacaaaa catgccttta
     1381 taaagttgat aataaaataa cacttattga ttcgtatctg gtggaatatc ctctgtatat
     1441 gcttcctgat ggaggtgttg aacaaaaagc tgaccagtgg tgggatgcca tctgtaaggc
     1501 cacaaagatg gtaatgaaga aaagcggtat tccacctgca aagattgctg ccatggcatt
     1561 ctgtgcacag atgcagggct ctgtgtttat tgacaaagat ggcaatgcat tgcgtaaccc
     1621 catgagttat ttagatcaac gtgctaccag gcaaattgaa aaatatctgt acaaaggaat
     1681 tgttaaaatt gaaaaatgga atgcttataa aactatcaaa tcattaattg taactggagg
     1741 gcttgctgca acgccaaaag acccattatg gaaataccac tgggtcaagg ataatgaacc
     1801 tgatattttt gcaaaagcat acaagtggct tgatgttaag gactatctga ttttcagatg
     1861 caccgggaaa ttgggtatga cacaggattc agcaaatgtc acatttattt atgatacacg
     1921 aaaaggaaga ctgggctggc ataaaggctt atgcaaaata tttgatgtaa acatggaaca
     1981 cctgccccct gtgttcaact caacagatac tatcggtact attacaaaaa aagctgccag
     2041 ggaattaggt attgctgaag gtattccagt ctttggcggt ggtggtgatg ttccacttac
     2101 tctggttggt tctggttgca ctgacctgta tgatacaaac atctatgttg gaaccagcgg
     2161 atgggtttca tctaacgttg ataaacgtat ggttgatatt ggcaacttta ttgcttcaat
     2221 cctgggagct attcccaatc attatgtgta tgttgccgaa caggaaactg ctggtgcctg
     2281 tttaaaatgg gttcgtgatc atttagcttt agatgaaata gggatgtatt tgcaggctcg
     2341 ccatattgca gacaaaagta aggaatacga atctttgtat gattttttga atacggttgt
     2401 aatgcagaca cctcccggtg cgggaaatgt actttttaca ccatggctgc acggcaaccg
     2461 ttcaccacgt gaagacccgt atgcacgtgg tatgttcttt aacctgagtc ttacaactgg
     2521 taagcggcag atgatacgca gtgtgcttga aggcgttgca taccacatac tctggatgct
     2581 ggaagcgatg gaaaaaaaga ttccccgtca ggaagtgctg cgatttgccg gtggtggtgc
     2641 aaaatctgat gtatggtgcc agattatggc cgatattacc ggcagaaata ttgaagctat
     2701 tgaaaatgaa atcaacgccg gtactgttgg cgcttcggtt gtttgtgctg ttggacttgg
     2761 aattttatct tcatttaatg aagcaaagcg cctggtggga gtgcgaaaaa tatttaagcc
     2821 acaaccacaa tacacagata tgtatactcg caattttaaa gtttttacca aactgtatga
     2881 aaagaacaaa gatcttttta aaatacttaa ttattagagt tcaggaggaa tacaatgaaa
     2941 caggaatatg ccatttcaca ctggccagat tcaaaagaaa ttcttgaaaa attgagggtt
     3001 cttgcaaatg aactaccggt gtatcagatt aaccgtaata aaatgaatga ataccttgaa
     3061 aagtatgaaa caaaatttaa atcatcaaag caattggtag aaaaagccag gcaacgaata
     3121 cctggtggtg tacagcataa cttagctttt aattatccat ttcctttagc aatagcaaaa
     3181 gctgatggtg cctacctata tgatgttgat ggcaataagt ttatagattt tcttcaggct
     3241 ggtggcccaa ctgtacttgg cagcaattac aaacctgttc gtgataaagt tatagaagtg
     3301 atcaatgagt gcagcccggt tacaggctta tttcatgaat atgagttact tctggcagag
     3361 cttatacact cacatattcc atctgttgaa ttattccgat ctcttgccag tggaaccgaa
     3421 ggatgtatgg cagcaataag ggctgcacga tgcttcaccg ataaaaagta tatcatcaag
     3481 gttggcggtg cctatcacgg ctggagcgat cagatggtat ttgcaatgcg aataccggga
     3541 ctgggaactc tggattctca tggcatacca gaaggttgct atacctacat gcaagaattt
     3601 tatccaaatg atattgaagg cttggaaaaa ctactgaaag aaaacgaatc aaagggtggc
     3661 actgctgctg ttatagtaga gccgctgggt cctgaaagcg gtacacggcc agtacattat
     3721 gattttaatg ctaaagtgcg tgaactatgc gataaataca attcactgct catctttgac
     3781 gaggtggtta ccgcttttag agttggactt ggcggtgcgc agggctattt tggtgttaaa
     3841 cccgatttaa cagtatttgg caaatgtgtt gcaggtgggt atcctgctgc aggaggtgtt
     3901 ggcggcagaa gagaaattat ggaaacgttt gcagcaggta ttcagggctt aaaaaagcgc
     3961 gccatggttg gtggcaccct atctgcaaat cctttgtcat gcgcagctgg atattttgcc
     4021 attaaagcca ttgaggaaac acaggcacac atcaaagcag gaaaagcagg tgataagctt
     4081 acacaaggat tacaggaaat atttaaacga tatgacctac cctttgtagc atttaatcat
     4141 ggctcaatat gccacatgga aactggtgct gccatgttaa tggaattaac caaccccaag
     4201 atatttgagc aggttggaca gcgtcgtaag gttatggaag agcttggggc agcacttatg
     4261 gttgaaggca tcataacact tgcaggaagc cgcatttaca caagtatggc tgacactgat
     4321 gatgtcattg atgatgcatt agagcgattt gacagagtat ttaaatattt tgaaccagtt
     4381 aaggagaaat gatatgactc atgaaataaa agtatacaat taccgctgga taattttgtt
     4441 tatttttgca ttactgaata ttgttattca gattcactgg atatcctttg ccagcattac
     4501 ttcagaagca gcggcgtttt acaatgtaac gccgctgtca attggctttt tatcaatgct
     4561 ctttatgatt gtctatgttt ttatatcact acctgcttca tatattatag acacgcatgg
     4621 gatcaagctt gggattggca ttggtgccgt actcattggt atatttggca ttataaaggg
     4681 tttgtatgca tattctttta atgcgatagt tactactcaa atagcgttag ccatagctca
     4741 gccatttatc ctcaatgcat atacacggct atctgcaaaa tggtttcctt tagatgagcg
     4801 tgcgacagca gccggcatcg catcgcttgc acaatatatt ggcataatag ttgggcttgc
     4861 tgctacgccg tttctggtac attcatattc tataccacac acaatgtata tctatggcat
     4921 cttaagtatt gccattgcag tactatttct tctttttatg aaagaagagc ctcctacacc
     4981 accgtgcgaa aaagggcagg aagaacgctt caaagtaaaa gaggggctac agcatatatt
     5041 cagcatgaaa cagatgtggc tggtaatact tcttttcttt ataggacttg gaatgtttaa
     5101 cgcagttaca acatggatag agcagatact tgagccacgt ggctttaatt ctgagcaagc
     5161 cggtattgtt ggtgccagca tgatggctgg gggtattctt ggagcaatta ttctgccgtt
     5221 actttcggac cattatagaa agcgcaaagt ttttttaaca atctgtatgg catttcttat
     5281 tccgggactt gcaggtttaa catttgcaca acattttaat acaattctga tttcaagttt
     5341 tattcttggt ttttttatca tgagtgcagg cccaattgga ttccagtatg cagcagagat
     5401 ttcctatcct gcacccgaat caagttcaca gggcctttta ctcttatctg gtcagatttc
     5461 aggaatactc tttatttttg ccatggatgc attcagaaca agcactggtg caatgacccc
     5521 atttatgatt gcctttattg tgctgatggt tattaataca ctattgacat ttaaattaaa
     5581 agaatcaaat ttaatcagct cataatattt ttaatgttat aatgacgtaa ttttttaaca
     5641 aacgaagaga gtggaatcat attcttaaag caaagattat tacactatgg taattcaaaa
     5701 taaataccat gaatatattt tatatcttta taggagttaa ccatgtcaca gtacacaatg
     5761 tacaaagaac aaattttaca atggtgccaa tggctcaatg aacacggatt ttttggtaca
     5821 caattgggca gtggtggaaa tatatcatgc agaattgaaa atgaagaaaa aattgcagta
     5881 actccatcaa gtctgcccta taaggatatg accacgagcg acatttgcat tgttgattat
     5941 aaccagaatg ttattgaagg caaaaagccc acaatggaat tgtcaatgca tcttgcaatt
     6001 tataaagcaa gggatgatat cagtgcagta gtacacactc atcagattta tgcaagcata
     6061 ctgtcggtca tgaatattga taagcctatc caagccatgt ttgatgaaat tgcatttgca
     6121 attggtgatg aggttgcttt tgttccctat gcactatcgg gcacacccga gcttgccagt
     6181 aatgttgctt ctgctgttac caataaatgt aaatgttata tcattaaaaa tcatggtgct
     6241 attgctttag gcaaaaattt agagcaagcc tgccttcatg ctgagatgct ggaaaaggtt
     6301 tccatggtgt attattattc actggccgca gggaaggaag ctagctcact ccccgaaccc
     6361 ataatgcagg taatacgcca gttatttggt aaatgatggt tgaagttcaa agtttcggtt
     6421 taatagtaac aaaatttttg cttgacatta tatgaaatat ttatagtatt tttataaaat
     6481 ataaaaatat cccctatact atatactatt ttacggaggt tttttatgaa aaaaagcgta
     6541 tcattacttg ttctttttat gctggtagca gcaataagca tagctgaagc aggtgttgta
     6601 cgaaacaatg caggttgtgg cgttggctca atgatttttg gcgacaaaga tggattgtta
     6661 tttgagatat tggcaacaac aacaaatgga atttgtggca accagacatt tggcatgact
     6721 tcagggacac tgggttgtgc tcctatgaaa gggattgttt caaacgaaaa gatcaacctt
     6781 tatgtagcag ataacatgga taaccttgca aaagacattg ctaaaggcaa tggtgaatat
     6841 ctggaaacat tagctctgct tatgaatgtt cccgaaagcg aaaaacagca gttcttcaca
     6901 aaattacagt ctaactttaa caaaatttat acttcaaacg atgtaacttc aaccgaagtt
     6961 gtaaagaata ttgaagcagt attgcagaat agttaatcat atcatatttt ttaatagggg
     7021 aatattgtca ctgctctcat acataaggca caataaagaa atctttatcg tgcctttttt
     7081 tattttatta atccatttta cagccatatt gttttatcac tatgcctatg ctcaacaaaa
     7141 atatccatat gaaacaatta ttgatatacc atccagcaca acaatatcaa aggatctttt
     7201 tcaatctata aatattgatc aattaatatc aattgcacac acaaaaaatt tatgtaatac
     7261 tgctcaatgg tgggctttgc ttcactacaa aaaaaatatc tttggaatta aaagtcttgt
     7321 tgatgacccc aaattttttc ttgcaccaga tgggaaatac aatccccaaa gtgaacttga
     7381 cgccacaata cgatatttat actcatacca tgaaaaagga aatcccattg atcatttcat
     7441 ggcacggtat gaatggttaa aaaaagaatt acaaacacca agtccatatg aagtgttctg
     7501 caaaaaatat caggagatag tatcattcaa agacacaatt aaagttaacc tggtattccc
     7561 cacaggatat atcaacagcc ctgcttcaat gtttgggcat actcttttaa cagtacagtc
     7621 tcagtataat ccagggctgc tgtcatcagc tataaattac gctgcaaata ccaatgaaac
     7681 atttggtcca ctgtttgcat tcagaggaat atttggattt tataagggtt acttctcaat
     7741 aatgccgtat tatatgaagg ttcaggaata cagtgacctt gaaagcaggg atatatggga
     7801 atatacatta aaccttaatc aggaagaagt caggttcatg ctttttcata tttgggagct
     7861 tgataatgta tattcagatt attatttttt tgatgaaaat tgttcattta atcttctata
     7921 tcttatggaa gttacccgtc ctgattcaca tcttaccaat aatttctttt tctgggtaat
     7981 tcctattgat accataagaa aagtaatcaa tgcaggattt gttgaaagca cttcattccg
     8041 gccttcaaga acaacaacca ttaattatat ggtactacaa ctcaatcctg atcaaataaa
     8101 agttgtaaaa tccatagcta accatgaaaa taaagcatta acaaatcttt taaattcatc
     8161 atttactgaa acacaaaaaa tacaaattct tgattgtgct tccgaattca ttcaatatcg
     8221 atatgcaaat aaagaatata cacaaaaaga atatcaggat ttatttatca atgtgttaaa
     8281 aatccgaagt tcttttccac aaacattatc ctatacaatt cctgatccac caagaccaga
     8341 gaccggtcat tattccagcc atatacgctt gcagggagga atctttgaca agaaacctta
     8401 tagtgaactg gtatacaggc catcgtatca tgacctgtgt gatgatcctt caggctttat
     8461 tccgggatca caaattatat ttggtgaaac agcaattcgc tatgactatg ataatcaaaa
     8521 actattattg caacgtttag aattaataaa tattgaatcc ctttcaccaa taaaccctct
     8581 gataaagccg ttaagctgga aagtaattac cggatttgac cgtgtacctt ttgcaagcaa
     8641 taatcaaaca ctggtgtatc aacttaatac aggaagtggt tataccattg aggtcttcaa
     8701 atggctgcaa atatatggca tgattgaatt ttcaggctta ctcaataatg gctttaaaaa
     8761 taatattgaa gctggccccg gagtttcagc agggttcgtg ttgcaacctt ttcaacattt
     8821 tcgtattagg ggttatatag ttgatacata ctatctttat ggagaagcat gtaatttctt
     8881 aaccatgaag gccatcatgc agtataatat aagtcaacga tatagcatgc gatttgaagt
     8941 ctcaagctgg tataatgaac agtggtataa tcagttttca gctgcattga ctttctattt
     9001 ctggtgatta attctatgaa ctatcgcttc catataattt tatgtatatg tttagtggta
     9061 acaggctgcc ttgacagtgt tatctatcac cctgatacaa tgacacccac taccagtgca
     9121 ctaccatttc ccgcagaaga tgttctcatc catctcaatg aaactgtcac tcttcacggc
     9181 tggttcattt cttcacaaac agctcgtttt actgtatgtt attttcacgg taatgcgggc
     9241 aacatatatg acagaattga actattaaaa cttttacatt caattccgct ttctattttc
     9301 ataatagatt accgcggata tggtaaaagc agtggcaaac ccagtgaaaa aggattgtat
     9361 gaagatgcaa tggctgcatg ggagtatttg actgatgtta aaaaaatatc accaaaagaa
     9421 attattttgt ttgggcgttc acttggtgga cctgtagcgt tgtactgtgc atcaagaaaa
     9481 aaccctgcag gcattataat tgattcaagt tttaccagta ttaaatccat ggtgcaatat
     9541 cacagctcag gatgcatgtc actgtttttt aaagaaagat atccagctat tgattacata
     9601 caaaaaatag ctatacctgt tctgatactt catagcaggg atgatgaaac tgcaccattt
     9661 tcaatgggac aggaacttta cagacaatgt ccatcaaaca aaaaatattt tgtagagttg
     9721 cgaggtggac ataataataa ttttcttgtt gatgcaccac tataccgtaa cagtataaaa
     9781 gagtttgttt tatcattaac cagtgaggag tgatttcact atgataaaaa ggtatgtagc
     9841 tctatttgta tgcttgttgt ttccactata ttctgaagca caggattgtg tgtccatatc
     9901 ttttaataca ttgcgaatgc ttgcagaaag ccgcattgac ccatgcccaa tatgtcagaa
     9961 gaaacttcaa aggcaagcac tgggacaaat tacaaaagat atattcccag gtaaaaaaat
    10021 aatgtttaat tcatcatgca gcctgataaa gactcagcaa acaaaagata acgaattaat
    10081 gctgaacaca gactatgatt caaaaattat gattaatgga atagaaacta atctgccata
    10141 tattatttta aagttttaca cacatgaaaa ccacattgtt ggaattgatg aaaaggatta
    10201 tacttcaagg gaaattcaga atttgtataa aaatctaatg tataataaac ctgttgaagc
    10261 agaagttatt gtcatttcct ataaatacag tgattttgca tcttcaatat atttgcccga
    10321 acgaaataca ttaattatcc attgtaaagt tatccgtttg caacagatac aataaattct
    10381 ttatttactc aattgcctct gccagcaatg aaatgggatg tattactgta tactgagtat
    10441 tcatctctat ctgccattta caggtttcac aatcggttac aacaaaatct ggttttgcct
    10501 cttcaattat tttaaagagt tcgcttccca ctgcctgaga aatggcataa tattcatttt
    10561 taaagccata tgtaccggat ataccacagc attcagaatg cagtatcttc agggttaatc
    10621 cgggtatctt acgcaacagt tcaattgtaa atgcaacatt gcccatgcgt tctaaatggc
    10681 agggtgaatg gtatgccaca gtatatttaa gaggttttaa attggggatg ttgccttttt
    10741 caaattcctc aagaacatat tgcgtaatat atgctatgtt gtttgcaata gggctgttat
    10801 caagttcaag caaattggga tattcatgtt tcaatgctaa cgaacagctt gatgatgctg
    10861 aaacaatcct ggtttgttca ttgggcagtg ctctggataa cacccgtata ttatgcctgc
    10921 catgcgattg tgcttttttt atatatccat ttgctattaa aggaacacca cagcattttt
    10981 ctttggtaat aataacaccc ttgcccaatg cattgagtac ttttactaca tcctttccta
    11041 attgaggata attgtaatta acgtaacacc catgaaaata tactaccttt tcaagatatt
    11101 tattctgata tttttttgct tctttaaacc attttctaaa tgtcatggat gcatagtttg
    11161 gaaatgttct gcctttagga atctggaaaa tcaggtcaag aaagtactta acaattccta
    11221 aatgggtaaa aaaattgact atgggtgaaa atattgtccc aaacgttccc attaaatcag
    11281 tacgactgag taaaaaatcc cgtatcctga attctttttt tgaatagcgc cattttgctg
    11341 tctggataat gtctgctatc tttacatcag atgggcatgc aatttcacac cgtttacagt
    11401 tagaacagta ctttaatgat atatcaagaa gctgtgggtt ttttatgcga agccgttcag
    11461 tatcaggccc tgactgcttg ggaccgggat acaaatgtgt aactcgtgca accgggcagt
    11521 aaatggtaca taccgtacat tttatacaat ggtcaaaact tatgttttct aactttatca
    11581 tggtgctatc tcataagctt atataaagat ataatctgat ttgcagcctt gtatgcggtg
    11641 cttatcgcca caccacttcc acagccttcc ctgattggat tataatgtgc aagtattgcc
    11701 ccactgcaat atacattctg gataatattg ttgttgctat catacggccg taaattttca
    11761 tcggtaacaa caccatactc taagaaagga tgagaatttt tgtaaaagaa ttgcgggcta
    11821 taccatttat tgcgttcagg ctcataatga agtttcagtt taaatattgg ctcatagata
    11881 ttattgaatt cacttatcat ccccccacta aaaaaactgc ccgttgatag aatatagaat
    11941 tttgcttcca gcggattttc tggatggttg gcagtataca ctgcctgcac cacatcatta
    12001 ttaatttctg cacgcacaac tttgtcgcca gcaatataca caccgcccag ctgtgcaaac
    12061 cgtgatttca gcgcattgtc aattctcaat ccaagaattg atggcggaag cgttggcact
    12121 tcataaatga gtaatctggt gcgactttgc aacaaattgt gtatctgatt gaagttatga
    12181 atccccatga aagctgggat aaccacaaaa tcagcacctt cggctgccag tcgtatagca
    12241 tcagcaattt tatatacatt ttcttcatct tcaaaaatac gggcaatgtc aatggatctg
    12301 aattcatgag gatgcctggt taaatcctga agctggggca tatcaatact tttagcaata
    12361 atttcacagt ttgcaaataa cgcatgcttt gaaagctgtg ctgcagctaa ctgaggatga
    12421 aaatcacgat agccttcaaa atttacaatg acaatttttt tcatgctctt aaaacggtta
    12481 tacacgtcct cattgaacac gcttcgttga gaaaagtacg ttggcttaag cgtccccagt
    12541 gcagtaacat ggaaatgatt gaggttttca ttatcaaaaa gatatacatt ttgagtttca
    12601 cattcctgtt taaaaaaatg caatgcttca ataattaatg gcttgccgca ttttgtatac
    12661 ggatgctggg cgatagttcc tgttaaggta tccaatgcct caaaaggctt atacaccacc
    12721 ttattgttta tatatcccaa tacatcaata gatccggaag agaaatgtaa tgcactcatt
    12781 cccgatgaaa tgattgcagt gtgcagccct tccttaacac accgtatacc tgcagttaat
    12841 ccggaaattc caccaccaat tattatacaa tcaaatttca ttgttcttct tcctcaacaa
    12901 gtcccaggcc aaataacccc tgataaatcc agtaggtaaa ttcaaattcc cgtaatccat
    12961 caccccacag taccggtttc atccctttaa aacgttcttc aaggaaatct tttaaaagtt
    13021 ttgttgatgt atccggcgta aattcaccaa attcagtgaa aagtcctgca gcacgataag
    13081 cacataactc gccctggcat ggccccatgc caatacgtgt tcttcgtcgt aaatctacaa
    13141 ggtcttttac tttgagattt ttaatggcat actcaacttc acccagtgta accatttcac
    13201 attcgcagat aagcccataa ttacgcttct gcaaactcag gatattgtga acacgttcac
    13261 cgtggcggta atgcgtggag ccaactactg aattgggaat acctgaaaaa tattttattt
    13321 tctttgtata gccaactctt ttctctgaac cgggaagggg tattttatgg gtaacacagc
    13381 ttttagtatt accaatcttt tttgccacaa gatctgttgt catttctgcc atgaggcgat
    13441 acgtcataag tttgccacca gctatggtaa tgagaccctg aacattatca cgttctttat
    13501 ggtcaatgag cacaacacca cggcttattt ctctgccatc catctcacca gaaactgcaa
    13561 caagcggcct taccccacaa tatgctcgca acactcgtgc cttggaaacg ttgggtatta
    13621 atttttcgcc atcagctaat agcacgtcta tctcatcatc atcaacaaca agttcatcaa
    13681 ttttgtcata ggacactttt ctggaagttg taccaattaa tgataccgta tcaccaggaa
    13741 ctattatatc accatccgaa ggcacacggc accggtttac cacaacattg ttaattctgt
    13801 aatcaataat aaccatggaa cccttggaag ggaacatctt cagttcaata cctgcagtag
    13861 aacataaatg ttgaccccac acaccagatg catttactat aattggtgca tacacctcaa
    13921 aatattcttt agttacctta tcataacact gtacacccgc tacctgattt tgtttaagca
    13981 tgatacgttc cacttctgta tgggtaaaaa ctattgcacc cctctccact gaatccaaaa
    14041 tatttgcaga agctagcctg aatgggtcta tggttccatc aggcacccat attgcagata
    14101 gtatattggg atttaagttt ggttctattg ccagagcttt ctttatactt atttctttag
    14161 catcaatacc agcaaattta cacgattcaa gcagcctgtt atgataggtt gggtcatctt
    14221 caggaagagt tacaaacaaa ccacctgttg gctcaataca atgccatgct atttttttta
    14281 atattcggtt ttcattaata cactcagttg ctgattcaag atctttaaca gcataacggg
    14341 cacctgaatg taaaaggcca tgatttcgac ctgttgtacc tgaggctata tcattttttt
    14401 caattaaaac tgcatttata ccgcggagag tgcaatcacg caacacaccg catcccgtag
    14461 ccccgccccc aattattatt acatcagttt ctaatgtctt cacgtctctt ccttttaaaa
    14521 ttatagcaaa tcaactataa ttgcactata cctgatttca aaattgtata gatcccgata
    14581 attcttataa tagaatactg tataatactg ctttttagga attatcattt gaaacctgat
    14641 atcttcaatc actgaatggc attaatgtaa taacgacctg catatgtagt gataaaatat
    14701 atggaattaa ttattgcgca tgttgcaggt aaatcataaa aatttaagat atacttctta
    14761 taaaaaaata ttgtcaatta atatattgat ttacaaattc aggtttaaac atgccccgta
    14821 taaatgttac cataaactta tcggttaatt catcaatctc aggttcagat aaacgaggaa
    14881 cagacaaaat tgcctgatag gctatcatta aaagaccacc aatcagtata cttgttgtta
    14941 tctgcaaatc cacgccatct cgtaaaacac ccagatgttt tgcaagccgt aaatcatgca
    15001 atatgtgctt gcgtaaattc ttttcaaatt cacctataag tttctgaata tgtggattaa
    15061 aaccaaaacc agtttttaac aaaatggaag tcagttcacg gtcattattg agaaatacta
    15121 ttgtctgttt aatcctatga cgaaaataag atactggatc taatgtttta atttcctgat
    15181 ggctgatgct tactactcgt ttccattcct caagagaacg aattaaaagt gataaaaata
    15241 agtcttcctt attgcgaaaa tactcatata tagtaccttt gccaatatga gcaactttaa
    15301 ttacatcctc aacataagcc tcataatagc ccttctcggc aaataccttt tttgcgcaat
    15361 caagaatatg ctgcctgcgc aactctgctt tcattgcaac ccctattccg tttattttta
    15421 taacattttg aatatttaaa aattctttat atttggtcaa taacttttta ttgatagtat
    15481 ttattgttca taattttgat ccaattttag cagtgcattc tttaactcat caagtgtaaa
    15541 tggtttttgc aaaaatactg agtgcttaat acctttcgtc tcatctcggt aagaatcatc
    15601 tgaatatcct gaaataaaaa ttactttcat atccggtata attaatttta atctgttaaa
    15661 cagttcaata ccattcatct gcggcataac tatatcagta attaatatat caatgttgtt
    15721 tttatagttc tctgctactt gaagcatctc aagaggatca ctaaatgcat caacgtcctg
    15781 atcaagtaac aaaagcatct tttttagcat atctaatatg atcaagtcat cttcaaccac
    15841 taaaatttta tttttagata taccaatacc tctatcttct gtcctaccat tatctcttac
    15901 atgaccagat aattgtacct ctggcaattg ctcaacatat ttagggaaaa agatatttac
    15961 gactgttcct ttacctggct cactatagac attaataaat ccattaaact gttttactat
    16021 gccatacact gttgaaagtc caagccctgt tcctttgcca acttcctttg ttgtgaaaaa
    16081 tggttcaaaa agcctttcca acgtttcctt atccatccct gaaccatcgt caataaattg
    16141 tatcatgaca tagtctccgg gtttacaccc gggatgtatt gtgcagaatt cttcatcaag
    16201 atatctattt ttagtaacga tctttatatt cccaacatct ttaatagcat cacgtgaatt
    16261 tgaagccaaa ttggtcagaa tttgaacaaa cgttgcggta tcaatccata tatcatatac
    16321 atcatctgcc agttccagtt gcacttttat gttttcgcca accattgccg ctaacatctt
    16381 ttcagttgac cggataatat aattaggatt tacccgtatt ggatttactt tctgctgccg
    16441 tgaaaatgtt aaaagctgcc gtactatttc agcacttcgt tttgtggcat ttgatatttc
    16501 ttctaaatct gaatatatag gattatcttt ttctattttg attttaatta actctgcata
    16561 ccctgaaata atgctcaata tattattaaa atcatgtgca atcccgccaa ccaatctccc
    16621 aattgcttcc aatttttgcg cttgccgtaa ctgctcctct aatttttctt tttgggcttc
    16681 cgcttcaagc tgatacgtca tatcttctac aatggccaat ccaccaacta ttttattttc
    16741 tacaatgagt ggtgaaaata atgcccttgc aggtgttatt ttatctgcag ttactgattt
    16801 gtaccaacca ttatattcag attgtatacc ctgcaggcac ttattaacag catcaacaac
    16861 ccgctcatca ggcaacttta gagtatttaa accaatcaaa gcttccctgg aagaaccaat
    16921 tatcttaaca aaattatcgt tacaggctgt aataactcca ttttcatcaa atgcatagat
    16981 ccccattggg gcattattaa aaatcaatct gaataattct tcacttttct ttaatgcctg
    17041 ttcggcctgt ctttttgcag taacatcatg aataattgag aataaatact gttttccttt
    17101 aatggtaatg gcactgctgt atacatcaac atcgcgtatg cttccatctt ctcttctatg
    17161 cttaaattca aaatacacac gtttacaggc tcgtgcctct tccatacgtg ccttaatctc
    17221 ttcgggtgtt aatgtgttaa tttgctgaat ttgcatcgat tgtaattctt ttctactcca
    17281 cccataaaac tccactgcag cctcatttgc atcaacaata tctcctgtat cgggatcaat
    17341 gataagcata actgctttac tgtttttaaa aatggttcta tatcgttctt cactttcttt
    17401 cagttcatgt tcaactttct tacgttgagt gatatctctt attgatccaa ataaagcagc
    17461 tattctaccc ttttcatctc tgattacccg gattttaata tctccccata caaccgtttg
    17521 atataaagta atgattctgc attcaactga aataaaatta ttgtcaccag gctcttcaaa
    17581 catcttgtca tataggtaaa catcatcttt atgaatatat gaattcaaag gtgtattaat
    17641 tatttcatcc tgtttatagc caagtacaac ctcaacattg ggacttatcc atgtaattgt
    17701 ctttgtagat ggatccatgc tgaagacaac agacggattg acactgagaa catcatgcag
    17761 aagatcagcg ctggcctgta aattttgcag tgccagtgtt ctattttcaa tggttttaaa
    17821 tataagagct atcgcacata aaccagaaga aagtactatt aaatactgga tgcctatagt
    17881 agctattaca gtaatcccac gtggccaggg taataatagc tggcacagta aaaccagaac
    17941 atgtgttata accgcaaata gaacgattcc atacagaggg atgtttgttg gttcattttt
    18001 aaaaattcga cgtacaacca atccactcaa ggctgaaaca atgaccgatg caacgccaac
    18061 ataaactccc ggaccaccca gaataatccg gtagcttcct gcaataaacg atgcaataag
    18121 tgatgcgata cctcctccat acaaacctga caatgctata acaactgatc gtccatcata
    18181 tattatccct tcctcaaaca caaggggggt tgacattgca gcaattgctg ataagccaaa
    18241 caataaacct gaaaaaattc tgtatgtatt tggataattt tttctgagtg taagaatggg
    18301 gccccatatt acactaaggg caataagtaa cgttatgttg tgtacaagtg ttaacagcat
    18361 ctaattttta ccatagaaag cactgtataa ttaatatcaa aactgcataa aaaaagcaag
    18421 aataataaat gtaacaattt tttatataac cattacaaac tggcaatgat
//
LOCUS       DAORYL010000060        18358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_2509_length_18358_cov_13.755614, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000060 DAORYL010000000
VERSION     DAORYL010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 18358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18358
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2509_length_18358_cov_13.755614"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="PLV81_10835"
     CDS             complement(<1..863)
                     /locus_tag="PLV81_10835"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOM10633.1"
                     /translation="MCCIIIDNALKEKVSIPGKLDADIHYIYPPYDELPGILHSLNAT
                     PENCNAVLCIVPSNACEEIQSIVESVDKSANHLYVGYVCVGNTCDATMMQDVLALYTE
                     GINAGEFEFIVLKIKFIARQHFEFKNLNRQHLHQLEDTQRDFDALINIGKALSIEKNP
                     DRLLKLILHLSKTITGADAGSIYLVEEVNGSKVLRFKHSHTFSKDLPYEEFTIPMDKN
                     SIAGYVAVTQQVLNIPDVYELGPDDPVAFNSSFDKAHNYRSKSMLVVPMINHINKTIG
                     VIQLINSKEDI"
     gene            complement(1074..2483)
                     /locus_tag="PLV81_10840"
     CDS             complement(1074..2483)
                     /locus_tag="PLV81_10840"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOM10634.1"
                     /translation="MKKSLFKKGAYVYIEGDEDADTVYIVDKGVVQLDRYNKNAPIYK
                     NILKDGEFFGVIASLCNKPRMESAIARVDSVVTTLTKQEFINLLSKKPQIAIKVLNYF
                     SNELRAYNEMMIALKDRGVELETPEEALYNHIRYFYNNGSNNHAYYCCKRYLELYPQG
                     LFASVVTTMLESIKDKVKGAIVPVIQSPYKIYTDQQMIFCEDEPGNEMYIIKEGKVKI
                     VKLSSESEIILSVLKDGDVFGEMALISDKPRNAAAISWGKTVLLPIKKESLVAVMEQM
                     PAITQLIFKELSHRIWFTYIQLESQLYDKPITRIYALLENKLVEKGVSLKSNDPVKLN
                     FGIDELFRMLGYVASRMGKTTNLLLSDSNLQFNVGETIVEKPSQLTARAKYYKSRDHM
                     AIVEGEEEEKKEAPQKEAAIAQGEKAETKEQVEEEIRTATVTADEALSFLHYDEKTQE
                     PATTQPQEELHTISEEIEI"
     gene            complement(2487..3035)
                     /locus_tag="PLV81_10845"
     CDS             complement(2487..3035)
                     /locus_tag="PLV81_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOM10635.1"
                     /translation="MKIIVPLAPGFEEIEAITIIDVLRRANLDVQSAAFSDTLVTGSH
                     GIVVKADTLLHKCNPVDYNCIALPGGMPGSENLKHNDDVIKFIKHIYSKGGFVAAVCA
                     APIVLGASEILYSKKATCFPGFEHELTGATIVNEPVVVDGTIITAKGPGCAIPFALTL
                     VGLIAGKEVQNTLKNTMQVYWM"
     gene            complement(3504..4037)
                     /gene="pth"
                     /locus_tag="PLV81_10850"
     CDS             complement(3504..4037)
                     /gene="pth"
                     /locus_tag="PLV81_10850"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOM10636.1"
                     /translation="MKIIACLGNPGKKYAGNRHNVGMIAGMEIAQKYSIVINKKEHDA
                     ITGNGSIEGYDCLFVLPQTYMNNSGIAVQKVLSYYKETPENLIVLHDEIELQFGHVKI
                     KFGGGHKGHNGIRSIQQHLGTPDFYRIRIGVGRPHDDTPVADYVLSDFTTEEKNHLHE
                     LTHTVHAILVDWLKASK"
     gene            complement(4034..4372)
                     /locus_tag="PLV81_10855"
     CDS             complement(4034..4372)
                     /locus_tag="PLV81_10855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOM10637.1"
                     /translation="MKITRKSIDGLVIIKVEGSLVTEHIKQFEKEVYSALEKNNNIII
                     DFSELSFICSAGLSLLIASHKKAEPKNLKIVITGCSEDIIKLFKLTELDQHLLIKNTI
                     EEARLYISSQ"
     gene            4776..5729
                     /locus_tag="PLV81_10860"
     CDS             4776..5729
                     /locus_tag="PLV81_10860"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOM10638.1"
                     /translation="MRAARIVAPRKFSVDDDPIPDCPDAHVRVKLLKAAICGSDFPYF
                     ANDYPKESYPLPPGYPGHECLGVIEASRVKHIKEGTLVMYYPPFLDAYKEYHVTPPSR
                     IQEIPNTLPLNELLMTQLLGAVSHCAFRIDKPYNKHVVVMGQGPVGLFFTALMKNFGA
                     RSIIGVDILDYRLSVSKLMGATHTINPEKEDLIESVKTITGGSLADIVIDAYGQNVAV
                     INKCFEIAKHNGQVAFFGICLEEAPRLQFNTFFRKELRMIASVGPDLSMDYPYALDMI
                     VKGAIDVTPILTHDLPFEDIQKGFEMAIQRADNAIKIVLSF"
     gene            5762..6223
                     /locus_tag="PLV81_10865"
     CDS             5762..6223
                     /locus_tag="PLV81_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10639.1"
                     /translation="MPRAKIYGYVAVIVSALLGIAFVYYAFIHFDMYSSLHFSSIDTG
                     AYWAVVTPIAVVTLFILATGFWVGYTILTIKVAPPMPEIVEKKDNSKFKAFLLCLVTA
                     ALAAWFVYGLLHHYYLAIAIPAACITLVILGMVFWVGIAIITTRSTLKKDK"
     gene            6230..7468
                     /locus_tag="PLV81_10870"
     CDS             6230..7468
                     /locus_tag="PLV81_10870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOM10640.1"
                     /translation="MRALFLCYRGNPFCGGQGIYLYNLTRELAQLGVEIDVIVGPPYP
                     DPMDEWATVYRLENLNMWAIKTKHFPYEKLIKLYKPWHFTDYVLTRFHIFPEMETFSM
                     KAFFLLRKLLKEKHYDIIHDVQCLGWGLLPMKGYGIPIVTTVHHPLTKDREADFLVDN
                     TMWEMVCTILFYPLTMQRIVINRLDRIITSSKEGIKALYEAFKLPAEKVSVVYNGMDV
                     EVFRNTGEKRKENTILFVGNTEDHKKGLRYLFDAMTMLPEDVKLIIVDDGPPKRLTAW
                     GWIQERHLEHRVTFTGKVDLGTLVHLYSTCTMLVMASLYEGFGLPAAEAMACNTPVVV
                     TSAGSLPEVVGDCGIIVPPKDPVALKEGILKLLHNKSLREELGKKGRKRVEEYFAWPV
                     AAKNTLAVYHDVIEQYRSTL"
     gene            7465..8697
                     /locus_tag="PLV81_10875"
     CDS             7465..8697
                     /locus_tag="PLV81_10875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOM10641.1"
                     /translation="MKICLLSYRGNPYCGGQGIYLMYVARELAKLGHEVHAIVGPPYP
                     FPIEGVTYHYIDNHNYFNVKDFVKPDKPFATLEPLNFYEFMASKFGIFPEIESFSIRA
                     YCKLRELLKTHSFDIIHDNQCLGYGFLLIKQFGIPLVSTIHHPLTIDRSTWFEYPSSF
                     FLKTKRILYYPLVMQKYVANKMDLIITVSHDSAKEIYNAFGVPVEKQRVVYNGMDSSI
                     FYPIKTRKRPNSLIFVGNVEDRKKGVIYMLKALTLTKHKVHLTIVDGGAPNRNYVPKW
                     IDKFNLNDRITFTGKIPLDKLVQLYAQTQIALCPSLYEGFGFPAAEAMACELPVIAAT
                     GGALPEVVGEHMKTGILVPPRDPYALAEAIDYLIENPSVRERIGKAARERVLKTFTWE
                     NAAKQMVTVYQEAIDAHR"
     gene            8684..9391
                     /locus_tag="PLV81_10880"
     CDS             8684..9391
                     /locus_tag="PLV81_10880"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOM10642.1"
                     /translation="MLTVDFDLLAVQDGQNTLDAGCGLGRHSFEFVARGAHVFSMDMD
                     MDCVRRTRFTLVHMRDSGQSKPGARFQVLVGDALNLPFRNNTFDRIICSEVMEHVSDD
                     NRACSELVRVLKPGGLIAITVPTYISELLYDTLTYEYFTSPGGHIRKYVPRKLAQIME
                     QNGLKIYAIDFRHSFHTIYWLIRCVVGLHLENQFFTRHYRNFLTRVLFSPFMQKVERF
                     FDNFFPKSIILYAQKPL"
     gene            9446..10408
                     /locus_tag="PLV81_10885"
     CDS             9446..10408
                     /locus_tag="PLV81_10885"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOM10643.1"
                     /translation="MALTPTPVRTLQHYLEEAIFVGQAHRLGVFKELHSKPDTADGLA
                     LRMQFDKRATWILLEALVELGYLVKNNDRYMPTEYCVSHLVDEQSPDYEGFFLQFLLY
                     LINPWKTLPHVLRTGAPDETSFKELSMHDFIKGMDSPWKKKLAPEIVDLCIAQCSDPT
                     MCIDIGGAPGTIAKEFVRRGIATIVFDLPQSLEVTQDELRTIPTIEVIAGDATVALPD
                     CTVDIAFLGNLCHGQSPEDNQKIISMCHDILNSNGIIAIFDNLRNESYLGATLALHML
                     TQSKKGNIYSREEYFEWLYNAGFSKPRIYQLSDKAWQLIIAKKH"
     gene            complement(10460..11515)
                     /locus_tag="PLV81_10890"
     CDS             complement(10460..11515)
                     /locus_tag="PLV81_10890"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HOM10644.1"
                     /translation="MNVATKVTGIGAYVPKNEIDNDYVISLLTKKSKPYISKDSFEEF
                     IKKTRDKLERVGSNTRYWCHEDEYCTDIAYHAARMALDDAHVEPEQIDLIIFTGMSKA
                     LVEPATAHILSNALGTVNANVLDTQDACTSFMKSMQLADALIKTHQYKKILIVTGERS
                     FDWADFTCKTVDEIKWKFASLTIGDAAGALLLEPTDEPEYTENTKHFKFYYKVYSAFV
                     TNCTIGLNHTIGERYRLFSHSTNLIKNGYAATYNLVHEVFSKDEWVDYSYDRLICHNV
                     GNVVDAYIKEILESANKYIEGRYIPFFSKYGNVASATLPLEMFLAKHENRLNRGDSIM
                     FIVPAAGVQCGIMFFQY"
     gene            complement(11547..13733)
                     /locus_tag="PLV81_10895"
     CDS             complement(11547..13733)
                     /locus_tag="PLV81_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOM10645.1"
                     /translation="MKSVHPAFIKYLKNFPLRLFLYTEAIAFCIMTPLIAYVAYRALY
                     LEGNQKTIYIMSVIIIVIISFIISIITIKKKTLALTTYFTMYIDGVDIDETIIDNAYN
                     CYYSLPKKHSIDTALRWVGSVATVIVVINIISNPSLTDNFNLIMLLFIFAIFSALLFY
                     LVPDRQLSYIASKGLFSDYNRFVQKSKIARFFSITVITLMTFLVLILLLTLHNTNVYQ
                     YNKQLSGQIELIAGFAESSLPKDVDYTINDKYEKIPETKKIYSIIPTFIDFYIISPTG
                     LVLASNKWDVEGKNVNRFIMHALSLSHPRMTMKYIKNNEWYYAVIKQLHNRSVFLVFE
                     YPVKLIDKEIFKTTAFMIIAIFCSLLIIGLFSYSIINNRLKIVITVKDVLLSLSNGEL
                     HQHICINSYDELGEIVVSVDRLIHKIREIVLYMKGISSELAVSADELKNTTEHFSINA
                     QNQAANSEEITATVEEVSAGIDNISKAASHQSGRLSDLLTTMGKLSGIITEINQKIHE
                     TLNQSIKMTSLAHQGGSALDNMKTTMNKIIDSSSQMTNVVGLINKISDQINLLSLNAA
                     IEAARAGEAGKGFAVVADEISKLADETSNSIKNIDALIIENKEEVLIGIDNVRRTEGI
                     IKTIIDDINTVNGMMKEIASFMAHQLDTNTNVNEIANDAMKKSEVIAYSSDEQRKASE
                     EIVKAITTINEISQQNAASAQQLFSNANGLSNMAETLRTAIDYFKD"
     gene            complement(14286..14652)
                     /gene="rnpB"
                     /locus_tag="PLV81_10900"
     ncRNA           complement(14286..14652)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLV81_10900"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(14656..15246)
                     /locus_tag="PLV81_10905"
     CDS             complement(14656..15246)
                     /locus_tag="PLV81_10905"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOM10646.1"
                     /translation="MASQHTMRVIGGSFKGRIIPFEKKLKGAEATSQRFKKALFSILG
                     EDLTGKNFLDLYSGTGQIGLEALSRGASLVVFNDSDRNSYQFVKDIVLMWKLSGRSLH
                     YNIDATRCISLCGERGVVFDVVFCDPPFEKIASIPAVYSNILQAISDSSIINEDSVIL
                     VQHYHTNIMPDTLGRLIKKDTRKYGTNSLSIYTVVL"
     gene            complement(15250..15456)
                     /locus_tag="PLV81_10910"
     CDS             complement(15250..15456)
                     /locus_tag="PLV81_10910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOM10647.1"
                     /translation="MKITEDMKLKDVITNHVQVAKVFKKYNLNCIGCKGADEDTIRIV
                     ATNYGLDVQQFVKELNEAIKEAKN"
     gene            15890..16078
                     /gene="rpmB"
                     /locus_tag="PLV81_10915"
     CDS             15890..16078
                     /gene="rpmB"
                     /locus_tag="PLV81_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOM10648.1"
                     /translation="MAKCEVCGKAVLFGHNVSHSNKKTQKVWHPNVQKIRVVEGNKVV
                     RKNVCTRCIRSGAVRKAI"
     gene            complement(16082..16669)
                     /locus_tag="PLV81_10920"
     CDS             complement(16082..16669)
                     /locus_tag="PLV81_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10649.1"
                     /translation="MNIVQLIDYFYNAIVDPEKLSQIIAVEDKKHFAISAIVIAVVVF
                     MDIVAQSLLTVQSDFFYYKLTYGFVALFLINIVCIVVYAGLLTITLQIAGYQASGGFI
                     LNALALAQVPRIFIVPVVLIFHSLYFAPVFFYSFGSLALILWSIIIAMRCISQRYSLE
                     AIKAIGYLAAPVIVAGVVGFLIFVAGVMVIIGLLS"
     gene            complement(16666..17595)
                     /gene="sppA"
                     /locus_tag="PLV81_10925"
     CDS             complement(16666..17595)
                     /gene="sppA"
                     /locus_tag="PLV81_10925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOM10650.1"
                     /translation="MDKNRRILIAILVILVFAVVLAIIQISITMQRQTTLGTRITLTE
                     GPGVGVVRVYGRIESTGDMLSQANADYVVKRLDELAQDSRIKAIVVRINSPGGTVAAT
                     QEIYNKILSLKKKNIPVVASMGDLAASGGYYIASACNAIVANYGTITGSIGVIAIAPN
                     LRGLFEKLGIRMNVIKSGKYKDILASYRELSPEELQLIQQIIDTSYQKFVHDVAVGRN
                     MAVSDIMPYADGRVFTGSQALEYKLVDELGTFEDAVNKARQLAKLPEDSAVYEDTASP
                     FERILATMSSLVKPKLEQYINTNYSLVEYSYLP"
     gene            17894..18160
                     /locus_tag="PLV81_10930"
     CDS             17894..18160
                     /locus_tag="PLV81_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10651.1"
                     /translation="MKKFFSLIFVIILFVSVTAFAADLVNKDSRSYSLEISSVGTLHT
                     SIGSNTTIMSGAPDGSTIKIKETGSTITVDGDNPVIIKDGKLSQ"
BASE COUNT         5577 a         3682 c         3266 g         5833 t
ORIGIN      
        1 tcaatgtctt ccttactatt aataagctgt attacaccaa tcgttttgtt aatatgatta
       61 atcataggta caacaagcat tgattttgag cgatagttat gcgctttatc aaatgatgaa
      121 ttaaacgcaa ctggatcatc aggtccaagt tcatacacat ccggtatatt gagtacctgc
      181 tgggttactg caacataacc agcaattgaa tttttatcca tagggatggt aaattcctcg
      241 tatggcaggt cttttgaaaa tgtatgtgag tgcttaaacc gcaatacctt actgccattt
      301 acctcttcaa ccaggtaaat acttcctgca tcagcaccgg ttatggtttt gcttaaatgc
      361 aaaattaact ttaacagcct atcaggattc ttttcaatag agagtgcttt tcctatatta
      421 atcaaagcat caaaatctct ctgggtatct tccaactggt gaagatgctg ccgatttaaa
      481 tttttaaatt caaaatgctg tcgggcaata aattttattt tgagtacaat aaattcaaat
      541 tcacctgcat taataccttc agtatacaat gcaagcacat cctgcatcat ggttgcgtca
      601 caggtattgc ccacacatac atagcccaca taaaggtgat ttgctgactt atctacgctt
      661 tcaactattg attgtatttc ttcacaggca ttagatggta ctatgcacaa tacagcatta
      721 caattttcgg gtgtagcatt taaagaatgt aaaattccag gcagttcatc ataaggaggg
      781 tatatatagt gaatatctgc atcaagcttt ccaggaatgg aaaccttttc ttttaatgca
      841 ttatcaatta taatgcaaca cataatacgt aatgatccca atcaaatgtc ctaaaaagat
      901 atttattact tatacatacg atatacaata aataaaaagt caatgtaaaa aatatttata
      961 ggcacatcta taaactgctt ttatccttaa aatttacaat aaaaaaaagc agttttatcc
     1021 tatgaatgtg ccaacaaggg aacatcctta aaaattagtt tttagaaatt ccattatatt
     1081 tcaatctctt cagaaattgt atgcagctct tcctggggtt gtgttgtagc tggctcttgc
     1141 gtcttttcat catagtgcag gaatgaaaga gcttcatctg ctgttacagt tgcagttcta
     1201 atctcttctt caacctgttc tttagtttct gctttttcac cttgagcaat tgctgcttct
     1261 ttttgcggag cttccttctt ttcttcttct tcaccttcta ctattgccat atggtcacga
     1321 gatttataat attttgctct tgcagtaagc tggcttggtt tttcaactat agtttcacca
     1381 acattaaatt gtaaatttga gtccgacaaa agaagatttg ttgttttccc cattctggat
     1441 gcaacatagc ctaacattct gaaaagttca tcaataccaa agttaagttt aacaggatca
     1501 ttactcttca atgatacacc cttttcaaca agcttatttt caagcagggc ataaatacgg
     1561 gtaattggct tgtcatacag ttgtgactca agctgaatat aggtaaacca tatacggtgc
     1621 gatagttcct tgaaaataag ctgggtgatg gcaggcatct gttccataac cgccaccagc
     1681 gattcttttt ttataggaag caacactgtt ttgccccagc taatggcagc cgcattcctt
     1741 ggtttatcag atattaatgc catctcgcca aatacatcac catcttttaa taccgaaagt
     1801 ataatctcac tttcactgga caatttaaca atttttactt tgccctcttt aataatatac
     1861 atttcatttc cgggttcatc ctcgcagaag atcatctgct ggtcagtata tattttgtac
     1921 ggtgattgaa tgaccggaac tatcgccccc ttaactttat ctttaatgct ttcaagcatt
     1981 gtggtaacta cactggcaaa aagaccctgg ggatacagtt ctaaataacg tttacagcag
     2041 taatatgcgt ggttgttgct accattatta tagaaatatc gtatatgatt gtacagcgct
     2101 tcttcagggg tttcaagctc aacaccccgg tctttgagtg caatcatcat ttcattatac
     2161 gcacggagtt catttgaaaa ataatttaac accttaatgg ctatctgggg tttctttgat
     2221 aaaagattaa tgaattcctg tttggttaag gttgtcacca cactgtccac acgtgcaatt
     2281 gctgattcca tgcgaggttt attgcacagg gatgcaatga ctccaaagaa ttcgccatct
     2341 ttgagtatat ttttatatat tggagcattt ttattgtagc gatctaactg cactacacct
     2401 ttatcaacaa tatagacggt atcagcgtct tcatcgcctt ctatgtagac gtatgctccc
     2461 tttttaaaaa ggcttttctt catatcctac atccagtaaa cctgcatagt attcttcaat
     2521 gtattctgta cttctttacc agcaatcaga ccaaccagcg tcagagcaaa agggattgca
     2581 catcccggtc cttttgcagt gatgattgta ccatcaacca caaccggctc attaactata
     2641 gttgcaccgg taagctcatg ctcaaagccg ggaaagcatg ttgctttttt gctatacaat
     2701 atttcactgg cacccagtac aataggtgcg gcacaaactg ctgccacaaa cccgcctttt
     2761 gaataaatat gttttataaa tttaatcaca tcatcattgt gttttagatt ttcacttcct
     2821 ggcattccac ctggtaacgc tatacaatta taatcgacag gattgcattt atgcaatagt
     2881 gtatcagctt taaccacaat gccatgggag ccggtgacca gggtatcaga aaacgctgca
     2941 gactgtacat ctaaatttgc ccttcgcagc acatcaatga ttgttatggc ttctatctct
     3001 tcaaatccgg gtgcaagcgg cacaattatt ttcatggtcc tcctccttcc taaaataaga
     3061 ttgattctaa agtgtcattt ttaatcacca aattgtcaga ttgagaaatt ctaacatgtc
     3121 atttcgaatt ccctgataag caagaaatct tttaaacacc agcagtcatt ctgaggagtc
     3181 ccgactttgt cgggacgacg aagaatctct tcgtttatag ctctgagatt cttcgcttcg
     3241 ctcagaatga cgtgatgtct agctcacaat aacagcttcc cacgtcattc tgaggagtcc
     3301 cgactttgtc gggacgttta tgctctgaga ttcttcgtcc cgatacaatc gggactcaga
     3361 atgatataga tttcttctca ctgccttcgc cagaatgaaa ttgcaaaaat gttcaacaca
     3421 gtaatgatcc ccaagaatag tacattaatt gatagtatta atgtactaca tacctgcaat
     3481 aagtcaatca aatgagctat cgcctatttt gaagctttta gccagtctac caggatggca
     3541 tgtaccgtat gtgtgagttc gtgtaaatga tttttttctt ctgtggtaaa atctgacagg
     3601 acataatctg caaccggcgt atcatcgtgt ggccggccaa caccaatccg tatccggtaa
     3661 aagtcagggg tccccaggtg ctgctgaata gaacgaatgc cgttatgccc tttatggcct
     3721 ccaccaaatt ttatttttac atgcccaaac tgaagttcta tttcatcgtg cagaacaatg
     3781 aggttttcag gggtttcttt ataatatgac agcacctttt gcactgcaat gccgctgtta
     3841 ttcatatagg tttgaggcag cacaaacaaa caatcatacc cttcaatact accgtttccc
     3901 gtgatggcat catgctcttt tttgtttatt acaatggaat acttttgggc aatttccatg
     3961 ccagcaatca tgccaacatt atggcggtta ccagcgtatt tttttccggg atttcctaag
     4021 caggctatta tcttcattgt gaggatatat ataagcgggc ttcttcaata gtatttttaa
     4081 tcaataaatg ctgatccagt tcagttaatt taaataactt tataatatct tcactgcatc
     4141 cagtaataac tatttttaag ttctttggtt ctgctttttt atgggatgca ataagcaggc
     4201 ttaaacccgc actgcatata aatgataatt cgctgaaatc aatgataata ttattgtttt
     4261 tttcaagtgc actatatact tctttttcaa actgtttaat atgttcagta acaagagaac
     4321 cttcaacctt tatgataaca aggccatcaa tgctctttct ggtaattttc attgcaccat
     4381 caaccccttg aattatctat tagcagtaaa tatataggca tttatcctat aatttccctt
     4441 ataattgtca agcaaaagat atttgaagct acatactaaa atttataatt ttgctgttac
     4501 tcatgtataa gtataagcct tttattaata aattcaaaga ttattttcaa ccaaaatgct
     4561 attactatta actatgggac aatacatgac attattatgg gtgattacct gcccaactga
     4621 ccacccacgg cattgtctaa ataatttgct ttaactttgc atatacataa taaaatgtca
     4681 aataaataag ttgacaataa ccccactcta taacactgtg tagttcttaa ttatttgtat
     4741 atattaattt actctattta caggaaattt tgaccatgcg cgctgcacga atagttgccc
     4801 ctcggaagtt ttccgttgat gatgacccca tccctgattg ccctgatgct catgtcaggg
     4861 ttaaactttt aaaagctgct atctgcggtt cagattttcc atactttgca aatgattatc
     4921 caaaagaatc ttatccacta cctcccggtt atcccggtca tgaatgtctg ggagttattg
     4981 aagcatcaag agttaagcat ataaaagaag gcactctggt aatgtattat ccaccttttc
     5041 ttgatgcata caaagaatac catgtcactc caccttcacg gatacaggaa atacccaata
     5101 cattgccctt aaatgagctt ttaatgacac agcttctggg tgcggtttca cactgtgcat
     5161 tcagaataga caaaccatac aataaacatg ttgttgtaat gggacaggga cctgttggtt
     5221 tgttctttac tgcacttatg aaaaactttg gtgcccgcag tattataggt gttgatattc
     5281 tggattatcg gttgagtgtt tcaaaactaa tgggagccac tcacaccatt aaccctgaaa
     5341 aggaagattt aattgaatca gtaaagacaa ttaccggtgg ttcactggct gatattgtta
     5401 ttgacgcata cgggcagaat gtggcagtta ttaataaatg ctttgagata gcaaaacata
     5461 atgggcaggt tgcttttttt ggtatttgtt tagaagaagc gccacggctg caattcaata
     5521 catttttcag aaaagaactg cgcatgatag cttctgttgg ccctgatctt tccatggatt
     5581 atccatacgc gctggatatg attgttaaag gagctatcga cgtaactcca attttgacac
     5641 acgacctgcc ttttgaagat attcaaaaag gttttgaaat ggcaatacaa cgagctgaca
     5701 atgcaataaa gattgtatta agcttttaaa tggtatactg ttaacaaaaa gaggttatat
     5761 tatgccacga gcaaaaatat acggatatgt agcagttatt gtttcagcgc tgctgggaat
     5821 tgcatttgta tattatgcat ttatacattt tgacatgtat tcatcgttac atttttcttc
     5881 tattgatacc ggtgcatact gggctgtagt tacgccaatt gccgtcgtta ccctttttat
     5941 tttagcaacc ggtttttggg ttggatatac aattttaacg ataaaggtgg ctcctcctat
     6001 gccagaaata gttgaaaaga aggacaattc aaaatttaag gcattcttgc tttgcctggt
     6061 taccgctgct cttgcagcgt ggtttgtata tgggttgttg catcattact atttagctat
     6121 tgctattcct gctgcatgca taacactggt tatcctgggc atggtgttct gggttggcat
     6181 tgcaataatt acaacacgtt caactctgaa gaaagataaa tagccattta tgcgtgcact
     6241 atttttgtgt taccgtggaa acccgttctg tggcggacag ggcatttatt tatacaatct
     6301 caccagagaa ctggcgcaac tgggtgttga gattgatgtc attgttggcc caccataccc
     6361 tgaccccatg gatgaatggg ctacggtata cagacttgaa aatcttaata tgtgggcaat
     6421 aaaaacaaaa catttcccct atgaaaagct tatcaaactg tataagccat ggcattttac
     6481 ggattatgta ttaacccgtt ttcatatttt tccagaaatg gaaacattca gcatgaaggc
     6541 tttttttctt ttgcgaaagc tcctgaaaga aaaacattac gatattatcc acgatgtgca
     6601 gtgtttaggc tggggacttt tacctatgaa aggctacggc ataccgatag ttactaccgt
     6661 acatcatcca ctgacaaaag accgtgaagc tgatttcctt gttgataaca ccatgtggga
     6721 aatggtgtgt accatactgt tttacccctt aaccatgcaa cggatagtaa ttaaccgcct
     6781 tgataggatt attacctctt ccaaagaagg tatcaaagca ttgtatgagg catttaagct
     6841 accagctgaa aaggtttcag tagtctataa tggcatggac gttgaagtat ttcgcaatac
     6901 cggtgaaaag cgtaaagaga acacaatcct ttttgtgggc aacactgaag accataaaaa
     6961 gggattgcgc tacctttttg atgccatgac catgctccct gaagacgtta agctcatcat
     7021 agttgatgat ggaccaccaa aacggctcac cgcctggggc tggatacagg agcgtcatct
     7081 tgaacacagg gtaacgttta cgggtaaagt tgatttaggc acactggtac atctgtacag
     7141 cacctgcacc atgttagtca tggcttcact gtacgaaggg tttgggctgc ccgcagccga
     7201 ggcaatggca tgtaatacac cggtagttgt aaccagtgcc ggttcattgc ccgaggtagt
     7261 tggtgactgt ggcataattg tacctccaaa ggacccggtt gctttaaaag aaggaatatt
     7321 gaaactatta cacaataaat cactgcgtga agaattaggc aaaaagggtc gtaagcgtgt
     7381 tgaagaatat tttgcatggc ctgttgcagc aaaaaataca ctggcagtgt accatgatgt
     7441 cattgagcag tacaggagca cgttatgaaa atatgtctgt taagctatag aggaaatcca
     7501 tattgtggcg ggcagggaat ttatttaatg tacgtggcac gcgaactggc aaaattgggg
     7561 catgaggtac atgccattgt gggcccaccc tatcctttcc ctattgaagg tgttacctat
     7621 cactatattg acaaccataa ttatttcaac gttaaagatt ttgtaaaacc cgacaaacct
     7681 tttgccacat tagagccttt aaatttttat gaatttatgg catcaaagtt tggcatattc
     7741 ccggaaattg aatccttttc tatccgggct tactgcaaat tacgtgaact tttaaaaact
     7801 cattcatttg atattatcca tgataaccag tgcttaggat atggattttt gctcataaaa
     7861 caatttggca taccattggt gtcaaccatt catcatccgt taaccattga ccgctccaca
     7921 tggtttgaat acccatcaag ctttttcctt aaaaccaagc gtattctgta ctatccgctt
     7981 gtaatgcaga aatatgtggc aaacaaaatg gaccttatca ttactgtatc acatgattcg
     8041 gccaaagaaa tttataatgc atttggtgta ccagttgaaa agcagcgtgt agtgtacaat
     8101 gggatggata gctctatctt ttatcccata aaaacccgta agcggccaaa cagcttaatc
     8161 tttgtgggta atgtcgaaga ccgtaaaaaa ggtgtcatct acatgctgaa agccctgaca
     8221 ttgacaaagc ataaagttca tttaaccatt gttgatggtg gtgctcccaa ccgcaattat
     8281 gtgcccaaat ggattgacaa gtttaacctc aatgaccgca ttacattcac tggaaaaatt
     8341 ccactggata aacttgtaca gttatatgca caaactcaaa tagctctgtg tccttcactg
     8401 tatgaagggt ttggctttcc agcagctgaa gccatggcat gcgagcttcc cgttattgca
     8461 gcaactggcg gtgcgctgcc cgaggttgta ggtgagcaca tgaaaacagg aatactggta
     8521 ccaccccgtg atccatacgc actggctgaa gccatagact acctcattga aaatccatcg
     8581 gtgcgtgagc gcataggcaa agcagcaaga gagcgcgttt taaaaacctt tacctgggaa
     8641 aatgcagcca aacagatggt aaccgtgtac caggaggcaa ttgatgctca ccgttgactt
     8701 tgatcttctg gctgtgcagg atggtcaaaa cacacttgat gctggttgtg gtttagggcg
     8761 gcattccttt gaatttgtgg cacgtggggc acatgtcttt tccatggata tggatatgga
     8821 ctgtgttcgc cgtacacggt ttacgttagt tcacatgcgc gacagcggcc agtcaaagcc
     8881 aggtgcccgt tttcaggtgc ttgttggtga tgccctcaat cttcccttcc gtaacaatac
     8941 ctttgaccgc atcatttgtt ccgaggttat ggagcatgtc agtgatgata acagagcctg
     9001 tagtgaactt gtccgtgtat taaagcccgg cgggcttatt gccataaccg taccaacgta
     9061 tatatccgag cttttatatg acacgctgac gtatgaatac ttcacctccc ccggtgggca
     9121 cattcgcaaa tatgttcccc gcaaactggc acaaatcatg gaacagaatg gtcttaaaat
     9181 ttatgccatt gattttcgcc attctttcca tactatatac tggcttatcc gctgcgtggt
     9241 gggccttcat ttagaaaacc agtttttcac caggcactat cgcaactttt taacgcgtgt
     9301 actgttctcg ccgtttatgc aaaaagttga acgttttttt gacaactttt tcccaaaaag
     9361 catcatactg tatgcacaaa aaccattata atactatggg cgatagttca tggtaaaaat
     9421 ccatatttga aaaaaggaag aatatatggc tctgacccca acacccgtgc gaacattgca
     9481 gcattattta gaagaagcca tctttgttgg acaggcgcac cgccttggcg tgtttaaaga
     9541 acttcacagc aagcccgaca ccgcagatgg acttgcgtta cgaatgcagt ttgataaacg
     9601 cgcaacatgg atactgcttg aagcgcttgt tgaattaggg tatcttgtaa aaaacaatga
     9661 caggtatatg ccaactgaat attgcgtgag ccatcttgtt gatgaacaaa gtcccgacta
     9721 tgaaggcttt tttttacaat ttttactgta cctcataaat ccatggaaaa ctcttcccca
     9781 tgtactgaga accggtgcac ctgatgaaac aagctttaaa gaattatcca tgcacgattt
     9841 tatcaagggt atggattcgc catggaaaaa gaagttagct cctgagatag ttgatctgtg
     9901 cattgcacaa tgctctgacc ccacaatgtg tattgatata ggtggtgcgc caggaaccat
     9961 tgctaaagaa tttgtgcgcc ggggaattgc aacaatagta tttgatcttc cccaatccct
    10021 tgaagtgaca caggatgaat tacgcacaat tcccactata gaagtcattg ctggagatgc
    10081 aaccgtggcg ttgcctgatt gcacagttga catagcattt ttaggcaacc tgtgccacgg
    10141 tcaatcaccg gaagataatc aaaagattat cagcatgtgc catgacattt tgaattccaa
    10201 tggtatcatt gccatttttg acaatttgcg aaatgaaagc tatctgggag ctacccttgc
    10261 cctgcacatg ctgacacaga gtaaaaaagg gaatatatat tcacgtgagg aatattttga
    10321 atggctttat aatgcaggtt tttcaaaacc caggatatac cagctttctg ataaagcatg
    10381 gcaactgata atcgcaaaaa aacattgatt attcttgcac aggcagcggg aatatcagta
    10441 atactatcat taattacaat caatattgga aaaacattat accacactga accccagcag
    10501 caggaacaat aaacataatt gaatcacccc tgttgagacg attttcatgc tttgcaagaa
    10561 acatttctaa tggcagggta gctgaagcta cattcccata ttttgaaaaa aaagggatat
    10621 atcgcccctc tatatattta ttggcacttt caagaatctc tttaatgtaa gcatccacta
    10681 cgttccctac attgtggcat atcagtctat cgtacgaata gtcaacccat tcatctttag
    10741 aaaacacttc gtgaacaaga ttgtaagttg ccgcatatcc atttttaatt aaattggtgg
    10801 aatgagaaaa taatctatat cgctccccaa ttgtatgatt aagaccaata gtacaattgg
    10861 taacaaatgc gctgtatacc ttataataaa atttaaagtg ttttgtattt tctgtatatt
    10921 ctggttcgtc agttggctcc aataaaagtg ctccggcagc atcgccaatg gttaaagagg
    10981 caaatttcca ttttatttca tcaacagttt tacaggtaaa atcagcccag tcaaatgatc
    11041 gctctccggt tacaatcaat atcttcttat attgatgggt ttttatcaat gcatctgcca
    11101 gttgcattga tttcataaaa ctggtacatg catcctgagt atccaggaca tttgcattta
    11161 ctgttccaag agcattgctc aatatatgtg cagttgccgg ctctacaagt gccttggaca
    11221 taccggtaaa aatgattaaa tctatctgtt caggctcaac atgtgcatca tccaatgcca
    11281 tccgtgcagc atgataggca atatctgtac aatactcatc ctcatgacac cagtacctgg
    11341 tgttactacc aaccctttca agtttgtccc tggttttttt tataaattct tcaaaagaat
    11401 ctttactaat atatggcttg cttttttttg tcagcagcga gatgacataa tcattatcaa
    11461 tttcattttt tggaacatat gcaccaatgc cggtaacttt tgttgctaca ttcataatta
    11521 tcctccacca tatatcactt tattttttaa tctttaaaat aatctatagc agtacgcaag
    11581 gtttcagcca tattgcttaa tccattggca ttagaaaaca attgctgggc actggcagca
    11641 ttttgttgtg aaatttcatt tattgttgtg attgccttaa ctatctcttc tgaagctttt
    11701 cgctgctcat ctgaagaata tgcaataact tctgattttt tcattgcatc atttgcaatc
    11761 tcattcacat tagtgttggt atccagctga tgagccatga aagatgcaat ctctttcatc
    11821 ataccattaa ccgtattgat atcatctata attgttttaa ttatcccttc agtgcgccgc
    11881 acattatcaa tacctatcag tacttcttct ttattttcaa ttatcagggc atctatattt
    11941 ttaatactat ttgacgtctc atcagctaat tttgatattt catcagcaac tactgcaaag
    12001 cccttccctg cctcaccagc tcttgctgct tcaattgctg cattaagtga taataaattt
    12061 atttgatcag aaattttatt tatcaaacct acaacatttg tcatctgact tgaactatct
    12121 ataattttgt tcatggtagt cttcatgtta tcaagtgcgc ttccgccctg atgtgcaagg
    12181 gaagtcattt taatactctg atttaatgtt tcatgaatct tttggtttat ttctgtaatt
    12241 ataccagaca gcttacccat agtagtcagt aaatcagaaa gacgacctga ttgatgtgat
    12301 gcggcctttg aaatgttatc aattccagct gaaacttcct ctaccgttgc ggttatttct
    12361 tcagaattgg ccgcctgatt ttgtgcattt attgaaaaat gctccgtggt attttttaat
    12421 tcatccgctg atacagccag ctcagaagaa atacctttca tatataacac aatttctctt
    12481 attttatgta ttaacctatc cacactcaca actatctccc ccaattcatc atatgaattg
    12541 atgcagatat gctgatgcaa ttccccattg gataatgata ataatacatc cttcacggta
    12601 ataactattt taagcctatt gttgatgata gagtaggaaa atagcccaat tatcaataaa
    12661 ctgcagaata ttgctatgat cataaatgca gttgttttaa atatttcctt atcaattaat
    12721 ttcaccggat attcaaaaac cagaaataca gaacggttgt gaagttgttt aataacagca
    12781 tagtaccact cattattttt tatgtatttc attgtcatgc gaggatggct taatgataat
    12841 gcatgcatta taaagcggtt aacatttttt ccttctacat cccatttatt tgaagccagc
    12901 accagaccag ttggtgaaat aatataaaaa tcaatgaaag ttggtattat gctgtaaatt
    12961 ttttttgttt cgggaatttt ttcatattta tcattaatgg tgtagtctac atctttgggt
    13021 aatgaagatt ctgcaaaacc agcaatcagt tctatctgcc ctgataattg cttattgtac
    13081 tgatacacat tggtattgtg caatgtaagt aacagaatta aaactaaaaa tgtcataagc
    13141 gttataacag tgattgaaaa aaatctggct attttacttt tttgaacaaa tcggttataa
    13201 tctgaaaaca aaccttttga agcaatgtag gataattgac ggtcagggac aagataaaat
    13261 agcagtgcag aaaaaattgc aaagatgaaa agcagcatga tcagattaaa attatcagta
    13321 agagaagggt ttgatataat attgatgact actatgactg tagcaacact tccaacccat
    13381 ctcagtgctg tatcaattga atgttttttt gggagagaat agtaacaatt atatgcatta
    13441 tcaataattg tttcatcgat atcaacccca tctatgtaca tagtgaaata tgttgttaat
    13501 gcaagtgtct ttttttttat agtaattatt gaaattatga acgatattat gacaatgata
    13561 ataacgctca ttatgtaaat agttttctga ttaccttcca gatataatgc tctatatgca
    13621 acatatgcaa ttaatggtgt cattatacag aatgcaatag cttcagtata aagaaataat
    13681 ctcaaaggga aatttttcag atatttgata aaagcaggat ggacggattt cataataaag
    13741 taacctcctc tccaattctt tttgtctata agacctgcaa aattctctgg gttagttaac
    13801 aataaaatag gcatatgcct atttcaagta aaaaatatta taaactaaaa aaaataaaaa
    13861 aataagtata tgcacattca tggatatttg tatatttatt atttttttcc tgcaaaaagg
    13921 gtattggcac aacgtaatat tgaacataaa aaaattaatt tactacaagt taataataaa
    13981 taataaaagc ataaaacaca agtgtgatag ccagaatgtt taaagaaaac agacagcatg
    14041 caatctcgta atttttttat aaaaagaaca tgataccctc agaacgtaaa aaaagatatg
    14101 aaaagagctg ggcaaagagt ttttagcagg attgggaaga agacactttc agagcgggct
    14161 ttgtattcaa caaggtaagc acaagcaaag cgcatgaggt tcttgcacga ttggttgatg
    14221 agcttatatt atagtagcga taaagaggaa aagatacatc tctaatatta aaaatatagc
    14281 gaaaaataaa gaggggtcca taagccggat tctgttttga cagccatcaa tcttgcatgc
    14341 atgttgccat gcatgtccac gcggcctacc cgcaacctca agcgggccac tttagtagcg
    14401 gttgcctatt tggccttgca ccggatgggg tttgcactgc cacctgcatt actgcagatg
    14461 cggtgagctc ttacctcgcc ttttcaccct tacccctgac ggggcggtat gttttctgct
    14521 gcactttcca tcgccttgcg gcgcccgggt gttacccggc atcctgccct gcggtgtccg
    14581 gactttcctc acagcgctgt acagacacaa tacagtgttt atacagcgtg cgtatgctgt
    14641 ctgtacccct cttacttata atactacggt atatatactt aatgaatttg tgccatattt
    14701 tcgcgtatct ttttttatta accttccaag ggtatcaggc attatgttag tatgataatg
    14761 ctgcacaaga attaccgaat cttcatttat aatgctacta tcagatattg cctgcaatat
    14821 gttgctatac actgcaggga tactggctat tttttcaaat ggtggatcac aaaacacaac
    14881 atcaaataca acaccccgtt caccacatag tgatatacat ctggttgcat caatgttgta
    14941 atgcagtgac cttccagata atttccacat aagaacaata tccttaacaa actggtagct
    15001 gtttctatcg ctatcattaa acacaaccag tgaagcaccc ctgctcaatg cttccagccc
    15061 aatctgaccg gtaccagaat acaagtccag aaaatttttg cctgttaaat cctcacccag
    15121 tattgaaaat aacgcttttt taaaacgttg actggtagct tcagctcctt ttagcttttt
    15181 ttcaaacgga ataatgcgcc ccttgaagct gccaccaata acacgcattg tgtgctgtga
    15241 tgccatacac taattcttag cctctttaat ggcttcattg agttccttaa caaactgctg
    15301 tacatcaagc ccataatttg ttgccacaat ccgtatagta tcctcatctg ctcccttaca
    15361 tcctatacaa ttcaggttat atttcttaaa aactttggcc acctgtacat gattggtaat
    15421 tacatctttc aatttcatgt cttcagtgat tttcatagta taaagtctcc caaaaataat
    15481 ttttcacctc acgtattaat atacatacgt aagggtaaag ctgcaatatt aaaatatata
    15541 tcaaaactca ccagtaaata taatttactt aaaaaattcc aataaaattg ttgcaatttt
    15601 tacaagtcat aaaaattatt gtctatcatt tttttaagag tactatctgt aattattgta
    15661 acatggttta caagcaaata accgtataac gccatcatta aagatggcat agatgtatac
    15721 tatctatttt tattcataat catatttttt gatacagcag cgtgttaata aaaaattgac
    15781 aaatcagcat tttttagcta ttatataggc aactatcagt tataaatacg tcctattcat
    15841 attgctaaat gctatcatat aatcattaat acaggagcag gagataacaa tggctaaatg
    15901 tgaagtgtgt ggaaaggctg tattatttgg gcacaatgta agccattcca ataaaaagac
    15961 tcagaaagtg tggcatccta atgtacaaaa aattcgtgtg gttgaaggca ataaagtagt
    16021 acgaaagaat gtatgtacac ggtgcatccg ttcaggtgct gtacgtaaag caatataaca
    16081 actacgataa aagcccaatt attaccataa caccagcaac aaaaattaga aagcctacaa
    16141 cccctgcaac aatgacaggt gcagctaagt aacctattgc cttgatagct tccagtgaat
    16201 accgctgcga aatacaccgc atggcaataa tgattgacca cagtatcagt gcaagtgacc
    16261 caaaactata aaaaaacacc ggtgcaaaat ataacgaatg aaatataaga acaactggca
    16321 caataaaaat ccgtggtacc tgcgcaagtg ccagcgcatt tagtataaat ccacctgatg
    16381 cctgatatcc tgcaatctgt aatgttatgg taagcaatcc tgcgtatacc acaatacaca
    16441 caatattaat gagaaaaagc gccacaaaac cataggtcag cttatagtaa aagaagtccg
    16501 actgcacggt taaaagggat tgcgcaacaa tatccataaa aactacaact gctataacta
    16561 ttgcacttat ggcaaaatgt tttttatcct caacagcaat aatttgcgaa agcttttcag
    16621 gatcaactat ggcattataa aaataatcaa tcaactgtac tatattcatg gcaggtagct
    16681 gtactctact aaagaataat ttgtgtttat atactgttca agttttggtt ttaccaatga
    16741 agacatggtg gcaagtattc tttcaaaggg gctggcagta tcttcataca ccgcactatc
    16801 ttcaggcaat tttgcaagct gtcgtgcctt gtttacagca tcctcaaagg tccccaattc
    16861 atcaaccagc ttatattcta aagcctggct tccggtaaat actctgccat cagcatatgg
    16921 cataatatcg cttactgcca tgtttcggcc aaccgcaaca tcgtgcacaa acttctggta
    16981 gcttgtatca atgatctgct gaatcagttg caactcctcc ggtgacagtt ccctgtatga
    17041 tgcaagtata tctttatatt tgccactttt gatgacattc atacgtattc ccaatttttc
    17101 aaaaagcccg cgtaaattag gagctatggc aataacgcca atggagccag taatggtgcc
    17161 ataattggca acaatggcat tgcacgctga agcaatataa tagccacctg atgcagctaa
    17221 atcgcccatt gatgcaacaa ccggtatatt ttttttcttg agcgatagta tcttgttata
    17281 aatttcctgt gttgcggcaa cagtcccgcc tggtgagtta atccgcacaa ctatcgcttt
    17341 tatgcgactg tcctgtgcca gttcatctaa acgtttaaca acataatcag catttgcctg
    17401 cgaaagcatg tcgcccgtgg attcaatgcg gccatatacc cgcaccactc caacaccagg
    17461 cccctctgta agagttatac gtgttcctaa cgtagtctgc cgctgcatgg taatactgat
    17521 ttgaatgata gccagtacaa ccgcaaaaac cagtataacc agtattgcaa tgagtattcg
    17581 cctgttttta tccatagtac gtttcctttt attttcgtta tggtactata atcagtctat
    17641 tttatgcaac attttttttc acaagggaat gtggttcaag ttttttttgg cgatagttac
    17701 tgtaataata ttttttgtct gttactattc ttttcccata tacatttatt caaactttct
    17761 tttatcctgg tggcaacaaa agaaagaatt tttttacaat atatatctat aaaattgttt
    17821 acttgactat accattcata ataattataa aaagcaaata catattttaa ttaaattaga
    17881 aatggaggta attatgaaaa agttttttag tctaattttt gttattattc tttttgtttc
    17941 ggtaacagca tttgctgcgg atttagttaa caaagattca cggagttact cccttgaaat
    18001 ttcaagcgtt ggaacactgc acacctctat aggcagcaat acaacaatta tgagcggcgc
    18061 acctgacgga tcaacaatta agataaaaga aacaggatca acaattaccg tagatggtga
    18121 caatcccgtc atcattaaag atggaaagtt gtcacagtaa atttcattct atttaattat
    18181 ccatctacag ggccgattgc tatgtgccct atttttttgt ctggatgata aaaaatattc
    18241 ttgacttttt cactacagtt gatatgtttt taactaaacg gttagttatg atgctccgac
    18301 cctttttttt acacccagcc tgcaatagaa taccgtttgc gatatgcctc atcatagc
//
LOCUS       DAORYL010000061        17991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_2565_length_17991_cov_13.047502, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000061 DAORYL010000000
VERSION     DAORYL010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 17991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17991
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2565_length_17991_cov_13.047502"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..929
                     /locus_tag="PLV81_10935"
     CDS             237..929
                     /locus_tag="PLV81_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOM10652.1"
                     /translation="MNILIFGPNGSGKGTQGAVVQKKFNLPHIESGVIFRENIKKGTE
                     LGVKAKAYIDRGDLVPDDITIPMILNRLQEADCKNGWLLDGFPRNVEQAKALDKALKE
                     AGMALDYVIEIVLDRDSAKKRIMGRRLCEKDNNHPNNIYIDAIKPAEKDGGFVCRVCG
                     GKLSTRADDQDEAAINKRHDIYYDTKTGTLAAVQYFKDISKQNGGKPKIIEVDGTPGV
                     KEVSEALLAKLG"
     gene            1060..1452
                     /locus_tag="PLV81_10940"
     CDS             1060..1452
                     /locus_tag="PLV81_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10653.1"
                     /translation="MSNVKIAYLSILIYTIFCIILSSIVYYFGGVAPGKGFAIGCLLS
                     LILTLLWIAGAIKGMKSNTLVLLSITAGGFILRLILLAVFAAGGVYILMMDLPTFAIA
                     FLIGTIFSLVLEVWFFTTLSGPQNPKFR"
     gene            1459..3066
                     /locus_tag="PLV81_10945"
     CDS             1459..3066
                     /locus_tag="PLV81_10945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOM10654.1"
                     /translation="MDTKTLQQLSQEELIKLVQQLQLKIDLLQKGVQEEPVDFKRLVE
                     AADDIIFVLDSEGNMVYRNRAMEDLFPVSRKDAIGMHFSLYLPAIELDRATTVFNQIL
                     FEGKSIHNEKMKTFDKDGNPVYLMANLAPIKDDEGNITGLFGILRNITEMHLMEKKLK
                     ESSRRLEEKIKEQLRQAEELKRLSTLNDEIINNSPIGIFTIDPTGIILTENPALRRII
                     GYGPDETRVGMNLTEMPGFSVDLKKYLEEIATQKKPVYIKNLTYQPSNQDKELTLNVR
                     ANPILDFDKKVKSILIMIEDATEQAQIQKRMQRAEKLSAMGLLAAGVAYELKVPINLL
                     TIDLNFIENNIPETSPVHDYVKSMKDELSRISQITEQLLNLGKPGDDDVETFEVHKLI
                     TSHPIQITLNRLQKSGFIINTAYPKESPKIKGRKNQLIQALLHIISNAEEAMPNKGEI
                     RINVGSVLKNGEKFASITIEDTGIGIPPENLSKIFQPFFTTKGPKSTGLGLMVTYTII
                     NNHGGAIGVKSAPGEGTSFKILLPAID"
     gene            3078..4547
                     /locus_tag="PLV81_10950"
     CDS             3078..4547
                     /locus_tag="PLV81_10950"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOM10655.1"
                     /translation="MKSQNPSQRNYLNDAESLLDQEGYIIIAPDEEKKDLQPEEKQTI
                     KTEHVTTQQMEKLPGKKPEILPKAIEAEATDIPSTIQYLKWHMERIAKNVIGREEIIK
                     QAMYAILTREHMLLLSRTGMAKSYLANYIFNTFDNVRVFASQASKEQTPDNYFGPYNI
                     DEFKKGRIRHNIHGSIIEANLVFLDEFFDASDVVLRSLLSVLNERKFINGPEQIDVAI
                     HTAIATANYMRMNEVTEAILDRFTYKSIIPEDDNVYQQLLIDHTYAFSRGKAIEPDKK
                     IPFNQIIYLSDIVRNKNKEVKIEIPDFIYFMKNVITNKFVSDMRKSDYKFFISPRKQA
                     KLQDFLRANALLNNRFEVTMDDLKDMYLALCTLNSFISVKAKDKSEKDVFLDAYQQTM
                     VHFNATGAIQQIEFLLNLRRIFQEIMENPEKKERLLASSSGILRGLKNLLKKLFPSRA
                     SDDEITLETLKRSLTELQPAVEEVRELKEGITKDYFAIL"
     gene            4553..6199
                     /locus_tag="PLV81_10955"
     CDS             4553..6199
                     /locus_tag="PLV81_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10656.1"
                     /translation="MSQVITTLQAPSRPSLDFFEYWDSLGLFEELSVILPPEFFVIFE
                     IAQVVFTPELISEALNAVEDISREEKIEAPNRDESILVNRVERISPLSDIMVIDRYRT
                     IYDLKKALPRELAWDDDIFDIKLFKHELLVQKFYEEKADTFKPISTLQDESGRQANRF
                     DQKFYLLLDRSRSMEIRMRSFFSKCLVAEFLRRKLNSNAKIFYRPFDSKPGDLFKIEK
                     KEDFPFLIERVLLTTTGGTSTNLQQAVFQAIDDIHYDKDMLNAEILVVTDGISKIDKY
                     LMKEKLGDIKLNILKIGRDLAEPDYFEMKKTFDANKIKFDPTALNIKEIQQKMEKSQE
                     GEEALSPLQKRAYRYILEYSETMFKDLKEISHRFVEIPDLDPSSLYELTDEKLDAIET
                     AVDAFTRIDFNTKTLEERKKLFKQAYFLSQYVSLLLENKKNSANPILKRCAYKLQKIT
                     QDILADPSLLQLVMETGKFSDKETLKLSKKQLKEKLKEMKMSQTPLSTEEIRKAQMLL
                     TSDLGEGNIGQFLRVLFIKLLQFIKRIVLKIKTIIVKKNN"
     gene            complement(6209..7174)
                     /locus_tag="PLV81_10960"
     CDS             complement(6209..7174)
                     /locus_tag="PLV81_10960"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOM10657.1"
                     /translation="MILVTGCNSLVGRTLIAKLLQDGEQVKVIDMWKGAKLPDAATFY
                     QSNLLDIDSIEEAMEDVDTVFHLMDVEDASYYGRRFMKKVNSKGTENLLKVAAEKNIN
                     HFIFLSSGKVYGNPDTMPIKEDDPLKPNTPYGKDKVKAEKLCLQFAEEGLPVTIVRPT
                     IITGPGVDDAMILIILYMALAMGDSNRLYIAGDGDSRYQLVHPDDVVAALLAIKHKQV
                     SKGKIYNIGSDNVPTQLEQVVKVKEIAQLDCQIKHITPFFTRVLSVLLKPLNIHYLRK
                     DHVQFILSNFVLDCNKIKEELNWVPKKTNIEIWAETIDWYRREKL"
     gene            complement(7268..8296)
                     /locus_tag="PLV81_10965"
     CDS             complement(7268..8296)
                     /locus_tag="PLV81_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10658.1"
                     /translation="MHNVYDKQFSYDHLIDQFHNTDTKINGLQLFYDNRDKVLSWFNY
                     DTLITIALYHFIDQLAYEIQEFPHNSDRYILDMLYRKAETYLAFMKGLQYYEQFLLIN
                     NLIHDDVLILWRHSLLGLRDRCINDFHEQKSLQYTITTALLTMPDESLIPFFYDIALS
                     SEPDIAISAIVGLALFRKKFANWKKLYKGDSDYDAMITVASSFDIQDYDYHNPPHNMY
                     ILFLYIRTSEIFASNVSEVLPLINTVLHAIPENHILYMRSIEAIESMLYRLTHREFNH
                     FTSDDIMNLISVFNTLPLEIVQNIVQYWNIPIMDFIYTVQRVIQEKQINLDDCSNIAT
                     LLCTAEFD"
     gene            complement(8378..8980)
                     /gene="lexA"
                     /locus_tag="PLV81_10970"
     CDS             complement(8378..8980)
                     /gene="lexA"
                     /locus_tag="PLV81_10970"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020774430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOM10659.1"
                     /translation="MKPLTKKQQAIFSFIKQTIKKTGFPPTVREIGNQFSITVKGAYD
                     HLKAIEKKGYLRCEAGKSRAIELLIDKEDVYADVRSIPLVGTIAAGIPILAQENIERY
                     IQLPSGFLPEGTLFGLKVKGDSMTGAGIYDGDIAIIRQQPSANNGEIVAALIDNEATL
                     KIFKKTERKVQLLPANEKYNPITTDTVTILGVLKALFRTY"
     gene            9338..10156
                     /locus_tag="PLV81_10975"
     CDS             9338..10156
                     /locus_tag="PLV81_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOM10660.1"
                     /translation="MLTLEESYTLLQVPKNASDAEIAKAFKKLALLYHPDKNPHRIEW
                     ANKAMATINVAYNTIMAHRFKDKSTVNEKVTPQKKEPKFKKEDILREDLLTQYFIQYR
                     EKAKDALYQYFQYNLYNLARRDMPANADIFKKIVGQLRRSYHGIDNLCEYTNDEEFLQ
                     HFNIFKELLFTFYKSSECLNIIDSYANILDVEAFRIYRQGDDYLLRSQKEIFYERHNR
                     GFFKKEQAITNLVKAIQLLQLTLARFPQSSWVVESQIKLEHALSIEKYLKLFFE"
     gene            10162..10719
                     /locus_tag="PLV81_10980"
     CDS             10162..10719
                     /locus_tag="PLV81_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOM10661.1"
                     /translation="MDIISTLGIAVALAMDAFSVSIASGLYIYKPYHKFYFRIAFHFG
                     LFQFAMPIIGYFGGVAIEPIIKNFDHWIAFGLLLFIGIRMIIASFSQEKASFKGDPSK
                     GFTLILLALATSIDALAVGLSFGVLHKPIIMPSIIIGITCALFSTLGVFLGRKASDII
                     GKRAELIGGCILIIIGLRILFAHMQ"
     gene            10830..11519
                     /locus_tag="PLV81_10985"
     CDS             10830..11519
                     /locus_tag="PLV81_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10662.1"
                     /translation="MKIQTYIITIVLILIAVQTGAEDYQYQYFQKEKEEFVPFSDYIP
                     KVRMHYKVVPHYVEDFYELYGMKLYYDENSLRKNIDRLKIALTCKFRHPSQALVKIQT
                     EDEYYKYRNLMFMHINMLILRNYLKIATRYDKQHIYFYDADYAKDIQESLKIAEQMYK
                     EALPYWYEAKKYADTASTVKITTNLGFIETERVKIIRGEVDFEKIINDHITRVKKKQN
                     NIDALLASYQK"
     gene            11524..12024
                     /locus_tag="PLV81_10990"
     CDS             11524..12024
                     /locus_tag="PLV81_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008869105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HOM10663.1"
                     /translation="MEVIEAIKSRRSIRHYTSKPVSDDVVVQIIEAGIWAPSGLNNQP
                     WRFVIVQSKDKKEALSQCTSYARIIKESQVCICVFYHIPSGYNRDKDILGIGACIQNM
                     LLAAHDKGLGAVWLGEILNKKQQVNELCSLSSDYELLAVIALGYPNEKGSSTRHPLEY
                     FIIKKI"
     gene            complement(12036..12485)
                     /locus_tag="PLV81_10995"
     CDS             complement(12036..12485)
                     /locus_tag="PLV81_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOM10664.1"
                     /translation="MDLFKKYITIFGLESQFTLEELSSAYRTLAMLYHPDISRDTDAL
                     QKMQLINTAYDYLKQHAVFLNKQNEIKNNNGKDSVYAVYKQAFTILQQAFYYYYTDGT
                     GFTGNKGYLVVKLKEAKALFSKIIKEYPYNEWVDDAIDKINSINKWL"
     gene            complement(12566..13651)
                     /gene="ychF"
                     /locus_tag="PLV81_11000"
     CDS             complement(12566..13651)
                     /gene="ychF"
                     /locus_tag="PLV81_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOM10665.1"
                     /translation="MGFTCGIIGLPNVGKSTIFNALTGAHAPMENYPFCTIEPNKGIV
                     PVPDERLHIIANILEKRDPIPTRIEFVDVAGLVKGASKGEGLGNMFLSHIRAVDAIVH
                     VVRCFTNSDVVHVEGEIQPLRDIEIINTELLLSDVEVILRAKEKIEKKAKSGDKESAH
                     RLEVIERLFQHINQGNMLNRIKLDSDELLVSNEYGLITAKPMIICANVDEQGNATDVY
                     TTLHDYAQKNGLGIVAISAKIEEEIVELPPDEQREYRQALGVDRSALERLITTAYSML
                     DLITYYTITTQLQAWTIKHGTTAQKASGIIHSDFEKGFIRAEVFHYDDLMNYKSLHAI
                     KEHGLLRSEGRDYIVHDGDIIHFLFSV"
     gene            13782..15149
                     /locus_tag="PLV81_11005"
     CDS             13782..15149
                     /locus_tag="PLV81_11005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM10666.1"
                     /translation="MAETILLSLTLLSFLFLCVAIANVNFKEGRYSVPIVLWVTASGA
                     IALVNVSHTYITLIIPVYPFVFALFIHALIKPLSRGDIYTYGISQLATIIALFLPFTI
                     MPVILAFIHIYTAFTIHKTISFKYGIKFLQGMLIIESVILGFISYMQNIQVLIITLTG
                     FITVFLTIMIYDTLKRSQTALLKIRALARMNANLTKLNRLLKQRNDQYIAMLNKKDND
                     IQLLSKYSTMAEITASIAHEIAQPLTGIKSIAQNMMDDINLDDFNYLEALSELQKICT
                     MVDKATYIIDHIRSFTTNNHIFKLIDINTIIMNAVTLTHNQLKKHNIDIVLLLNDSIP
                     KIYGNSINIEQILLNLITNARDAIIEKAKKMQSDYTGKITIKTDYSDDYVIVIIEDNG
                     IGIQPDIQKHIWSPFFTTKKYSQSLGIGLSISKKIINDHNGEISIDSTPDEGTIFIIK
                     FSLLK"
     gene            15249..16949
                     /locus_tag="PLV81_11010"
     CDS             15249..16949
                     /locus_tag="PLV81_11010"
                     /inference="COORDINATES: protein motif:HMM:NF017106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P83/100 family protein"
                     /protein_id="HOM10667.1"
                     /translation="MLYPYKLILFIMFVFLITFIQLNATALEVAIDEIRTEPVIFVNY
                     QGAYKKADSLQDIENIGKSLARNSNNTGIIRFGMKYSLINAISEEEPKKFSATIFSID
                     KKAKVGHIDIIRKILSAYLQQRYGYSQQHAKAIALFLTYYNAIYRGNIDYFSSKYKSI
                     VIKYITKYNAGISTKYYEWPGATKMLIPLTEQPQKGKLDSILIDIISDKKILQEIRKD
                     DKNIQARKDMVDIKEKILTQEKVDLEKSKKDVIQETTTLEENKKSIEQKKEELQQTEK
                     EINKMKEEIKKEPSPEKQKDIQKDIETKEQQLQKQKEDIKKKEQEIAKKESEILDKKN
                     KVSEKEERLAEKEKALEKEKEEIAADEIKKAPPKEKELVKKLEKKEKELDEREDKLRS
                     KELDQNIYADKLYYLKIKEYLQDGHYNNEMVMIDPASRKILFKSPEKNICGSRYDIFS
                     GGVVVITHKGSHAIGHRLTLLDRNSLEAKLYGSDNIFWRSFIIIKDGFIYAIVIENGK
                     FYLGRFDTNLKMVSKSSEEVNENTFISFWDNFIYINRVDKTIMVLDKENLTLIDTIKP
                     "
     gene            complement(17018..>17991)
                     /locus_tag="PLV81_11015"
     rRNA            complement(17018..>17991)
                     /locus_tag="PLV81_11015"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         6114 a         3372 c         3166 g         5339 t
ORIGIN      
        1 tatacatttc atttttataa agaaaacggt atacattgct gccagttcca ttggggacca
       61 ccccctgaag tgctccaaca aacagcataa cttatcatcc ctagttaatg tttactgagc
      121 tatcgcctac ataaaaactt actttacaat tatatacagt tttttactgt tgctatgaaa
      181 tgattttcta tgctgaatgt ttcagctata cacatactaa tccaagaggt ggtaccatga
      241 atatactaat atttggccca aacggcagcg gtaaagggac gcagggtgcg gttgtacaaa
      301 aaaaatttaa tcttccgcac attgaatcag gtgttatttt cagagaaaac attaaaaaag
      361 gcaccgagct tggtgttaaa gctaaagcgt atattgaccg tggtgacctg gttccagatg
      421 atatcactat ccctatgata ctgaaccggt tacaggaagc tgactgcaaa aatggctggc
      481 ttttagatgg gttcccgcga aacgtagagc aggcaaaagc cctcgataaa gcactaaaag
      541 aagcaggcat ggcccttgat tacgtcattg agatagttct ggatagagat agtgcaaaaa
      601 aacgcattat gggcagaaga ctgtgcgaaa aagacaataa ccatccaaat aacatataca
      661 ttgatgctat caagcctgca gaaaaagatg gcggttttgt gtgtcgtgta tgtggtggca
      721 agctttcaac acgtgctgat gaccaggacg aagcggctat taacaagcgt catgatatat
      781 attatgatac aaaaacagga acattagccg cagttcaata cttcaaggat atttcaaagc
      841 aaaatggtgg taaacctaaa attattgaag ttgacggtac tccgggtgta aaagaagtaa
      901 gtgaagcact tctggcaaaa ttagggtaac atatcgtatt caggaactgt cttgtgacag
      961 ttcctgtttt taaaataaaa aatattagtt gacctgtcac ggctaaaaat tatacattat
     1021 aaatttgtat atagtgctta atacaggata ggtcaatcta tgagtaacgt aaaaatagca
     1081 tacctcagca tacttattta tactatcttt tgcattattc tttcatcaat tgtatattat
     1141 tttggaggag tggcaccagg aaagggattt gcaatagggt gcctgcttag ccttattctt
     1201 acattgctgt ggatagcagg agcaatcaaa ggcatgaaat ccaatacgct ggtactgtta
     1261 tcaattacag caggtggttt tattttgcgg cttattcttc tagctgtgtt tgcagcaggt
     1321 ggtgtttata ttttaatgat ggaccttcca acgtttgcta tagctttcct tattggaaca
     1381 attttcagcc tggttcttga agtctggttc tttaccacat taagtggccc tcaaaatccc
     1441 aagttcaggt aagtaattat ggataccaaa acactacaac aactttctca ggaagaactc
     1501 atcaaactgg tacagcagct tcagttaaag attgacctgc ttcaaaaagg agtgcaggaa
     1561 gagccagtgg attttaaaag attggttgag gctgccgatg atattatttt tgtactggac
     1621 agtgaaggga atatggttta ccgcaacagg gcaatggaag atttgttccc ggtaagcagg
     1681 aaagatgcca ttgggatgca tttttcgtta tacttacctg ccattgaact tgatagagcc
     1741 acgacagtat tcaatcaaat attatttgaa ggtaaatcaa tacacaacga aaaaatgaag
     1801 acatttgaca aggatgggaa tcctgtatac ctgatggcaa atcttgcacc tatcaaagat
     1861 gatgagggaa acatcaccgg cctttttggc attttgcgca atattaccga aatgcactta
     1921 atggagaaaa agcttaaaga aagttctcgc aggcttgaag aaaaaataaa agagcaacta
     1981 cgtcaggctg aagaattaaa acggctatca acattaaatg atgaaattat aaacaattcc
     2041 cctattggaa tctttaccat tgatcctaca ggcattattc ttactgaaaa tcctgcattg
     2101 cgccgcatca ttggttatgg accggatgaa acacgagtgg gcatgaactt aaccgaaatg
     2161 cccggtttca gcgttgactt gaaaaaatat ttagaagaga tagctactca gaaaaaaccc
     2221 gtgtatatta aaaatcttac ctaccagcct tccaatcagg acaaagagct gacactcaat
     2281 gtacgggcta atcccatcct ggactttgat aaaaaggtaa aaagcatttt aataatgatt
     2341 gaagatgcaa ctgaacaggc acagatacag aaacggatgc aacgagccga aaagctttct
     2401 gcaatgggat tacttgctgc gggggttgct tatgaactca aagtgcctat taacctgttg
     2461 actatcgacc ttaactttat tgaaaacaac atacccgaaa caagtcctgt ccatgactac
     2521 gttaaatcaa tgaaggatga gctttcccgc attagtcaga ttaccgagca actgctaaat
     2581 ttaggaaaac ctggcgatga tgatgttgaa acatttgaag tacataaact aataacaagt
     2641 catcccattc agataacact gaaccgtctg caaaaaagcg gatttattat taacacggca
     2701 tatccaaaag aaagccccaa aatcaaaggc agaaaaaatc agcttataca ggctttattg
     2761 catatcataa gcaatgctga agaagcaatg cccaataaag gggaaattcg cattaatgtt
     2821 ggcagcgtac tgaagaatgg tgaaaaattt gccagcatta ccattgaaga caccggcatt
     2881 ggcattcctc ctgaaaatct ttcaaagata tttcagccat tttttacaac aaaaggtcca
     2941 aaatctacag gattgggact gatggtcacc tacacaatta taaacaacca tggcggagca
     3001 ataggagtta aaagcgcccc tggtgagggt acttcattta aaatactact tccagctatt
     3061 gactaaaggg ggactatatg aaatcgcaaa acccttcaca aagaaattac ctcaatgatg
     3121 ccgaatcact ccttgatcag gaaggatata ttatcattgc acctgatgaa gaaaaaaagg
     3181 atttgcagcc agaagaaaag caaacaataa aaactgagca tgtcacaacc cagcagatgg
     3241 aaaagctgcc tggcaaaaaa cccgaaatac tgcccaaagc aattgaggct gaagcaaccg
     3301 atattccaag caccatacag tatcttaaat ggcatatgga acgtattgca aagaatgtaa
     3361 ttgggcgtga ggaaattatt aaacaggcaa tgtatgctat actgacacgt gagcatatgc
     3421 ttctgttatc gcggacaggt atggcaaaat cgtatctggc aaattatata ttcaacacat
     3481 ttgataatgt acgagttttt gcatctcagg caagcaagga acaaacccca gacaattact
     3541 ttggaccata taatattgat gaattcaaaa agggacgcat ccgtcacaat atacacggtt
     3601 caataattga agcaaattta gttttcctgg atgaattctt tgatgccagt gatgtggtat
     3661 tgcgttcatt gctatcggta ttgaacgaac gaaaatttat aaatggccct gagcagattg
     3721 atgttgctat acacaccgct atagccaccg ccaactacat gcgcatgaat gaagttaccg
     3781 aagcaatttt agaccgcttc acctataaat caattatccc tgaagacgat aatgtatacc
     3841 agcaattgct gattgatcat acgtatgctt tcagcagggg caaggcgata gaacctgata
     3901 aaaagattcc atttaatcag attatctatc tgtcggatat tgtgcgtaac aagaataaag
     3961 aagttaagat tgagataccc gatttcattt acttcatgaa aaatgtcatc accaacaagt
     4021 ttgtaagcga catgcgaaaa tccgattata aattctttat tagcccgcgt aaacaggcaa
     4081 aattacagga tttcctcaga gccaatgcac tccttaacaa ccgttttgag gttaccatgg
     4141 atgaccttaa ggatatgtat ttagcactgt gcacactgaa tagttttatc tcggttaaag
     4201 caaaggacaa atcagaaaag gatgtatttt tagatgcata ccagcaaacc atggtacact
     4261 tcaatgcaac tggtgcaatt cagcagattg agtttttgct taaccttcgc cgtattttcc
     4321 aggaaattat ggaaaatcct gaaaagaaag aacgcctcct tgcttcatcg tcaggtatac
     4381 tgcggggact gaagaatctt ttaaagaaac ttttcccgtc acgtgcaagc gatgatgaga
     4441 ttaccttgga aaccttgaaa cgcagtttaa ccgaactgca gcctgcagtt gaagaagttc
     4501 gcgagctgaa agaaggtata accaaagatt attttgctat attgtaaatg ccatgtcaca
     4561 ggtaatcaca acattacagg caccttcacg acccagttta gatttttttg aatactggga
     4621 ttcattgggc ctatttgaag agctatcagt tattttgccc ccggaatttt ttgttatctt
     4681 tgagatagct caggtagtgt ttactcctga attaatatcc gaagcattga acgcagtaga
     4741 agatatttcc cgcgaagaaa aaattgaagc acccaaccgc gatgaatcaa ttcttgttaa
     4801 ccgtgtggaa cgcatatccc cattaagcga tattatggtc attgaccgat accgcaccat
     4861 ctacgattta aagaaagctc tgccacgcga acttgcatgg gatgatgata tttttgacat
     4921 taaacttttc aagcatgaac tgttggttca aaaattttat gaagaaaagg ctgatacatt
     4981 taaacccatt tcaacattgc aggatgaaag tggtagacag gcaaaccgtt ttgaccaaaa
     5041 attttacctg ctgttagacc gatcccgatc aatggaaatc agaatgcgct catttttttc
     5101 caagtgtctg gttgccgaat ttttacgtcg taaattaaac agtaatgcaa agatttttta
     5161 caggccattt gattcaaaac ctggcgacct gtttaagatt gaaaaaaaag aagattttcc
     5221 atttttaatt gaacgcgtat tgctcaccac tactggtggt accagcacca atttgcaaca
     5281 ggcagtattt caagccatag atgacatcca ttatgacaaa gatatgctta atgctgaaat
     5341 acttgtggta accgacggta tcagcaagat tgataagtac ttaatgaaag aaaaattagg
     5401 cgacattaaa ttaaacatct taaaaattgg ccgcgattta gctgaacctg attacttcga
     5461 aatgaaaaaa acgtttgatg ctaacaagat aaaatttgac cccacagcgc ttaatattaa
     5521 agaaattcag caaaagatgg aaaagtcaca ggaaggagag gaagccctat cgcccttaca
     5581 gaaacgtgca taccgataca ttctggaata ctccgaaacc atgtttaagg atttgaaaga
     5641 aatttcacat cgttttgtag aaatacctga tcttgatcca tcatcactgt atgaacttac
     5701 cgatgaaaaa ttggacgcca tcgaaaccgc tgttgatgca tttactcgca ttgattttaa
     5761 tacaaaaaca ctggaagaaa gaaaaaagct ttttaaacag gcatattttt tgtcacaata
     5821 tgtatcgtta ttgctggaaa acaaaaagaa tagtgcaaat cccattctga agcgatgtgc
     5881 ctataaattg cagaagatta cccaggatat tttagctgac ccttcattat tacagcttgt
     5941 gatggaaaca ggaaaatttt ctgacaaaga aacgttgaaa ctttctaaaa aacaactcaa
     6001 agaaaagctt aaagaaatga agatgtccca gacaccactt tcaactgaag aaatacggaa
     6061 agctcagatg ctgcttactt ctgatttagg tgagggaaat attggacagt ttttacgggt
     6121 tctttttatt aagcttttac aatttattaa acgtatagtg cttaaaataa aaacaattat
     6181 agtgaaaaag aacaactaaa catacatact atagtttttc tcttctgtac cagtcaattg
     6241 tttctgccca tatttcaata ttagtctttt tggggaccca gttcagttcc tctttaattt
     6301 tattacaatc aaggacaaaa ttggataaaa taaactgtac atggtctttc cgcaaataat
     6361 gaatgtttaa tggctttagt aaaaccgata gtacccttgt gaaaaagggc gttatatgtt
     6421 taatctggca atctaactga gctatttcct taaccttaac cacctgctca agctgagtgg
     6481 gaacattatc agaaccaata ttatagattt tgcctttaga tacctgctta tgtttaatag
     6541 caagtaacgc agcaacgaca tcatcgggat gtacaagttg atatcgggaa tcaccatcac
     6601 ctgctatata taatctgttg gaatcaccca ttgccagtgc catatacaga ataattaaaa
     6661 tcatagcatc atcaactcca ggccccgtta taatggttgg ccgtacaata gtaactggca
     6721 acccttcttc agcaaattgt aaacacagtt tttcagcttt aactttgtcc ttgccatacg
     6781 gggtattggg ttttaacgga tcatcttctt ttataggcat ggtatcggga ttcccataca
     6841 ctttaccact ggataaaaat ataaaatgat ttatattttt ttctgcagca acttttaaaa
     6901 ggttttcagt ccctttacta ttaacttttt tcataaatcg gcgaccataa tatgatgcat
     6961 cttcaacatc catcaggtga aaaaccgtat caacatcttc catggcctct tcaatactat
     7021 ctatatctaa aagattgctc tgataaaaag tggcagcatc tggtaatttg gcacctttcc
     7081 acatatcaat gactttaacc tgctctccat cctgtaaaag ctttgctata agtgtacgac
     7141 ctaccaaact gttacaacca gttaccagaa tcatgctgcc ctccatatca tagactgtat
     7201 gtccattcat actatagtag tgtttttttt agtcaagcgt aaaataagga attcatgatg
     7261 tactatatca atcaaattca gcagtacata gaagcgttgc aatattagaa cagtcatcca
     7321 ggtttatttg cttttcctga ataactcgtt gaaccgtata tataaaatcc attataggga
     7381 tattccagta ttgtacaata ttctgaacta tttccaaagg cagggtattg aaaactgaaa
     7441 taagattcat gatatcatca ctggtgaaat gattaaactc cctgtgtgtt aatctgtata
     7501 gcatactttc aatagcttca atagaacgca tatacaaaat atgattttca gggattgcat
     7561 gcaaaacagt atttatcaaa ggtaaaactt cagaaacatt actggcaaaa atttcagatg
     7621 ttcggatgta taaaaataat atatacatat tatgaggtgg attatgataa tcataatcct
     7681 gtatatcaaa ggaagatgct actgtaatca tagcatcata gtcactatca cctttatata
     7741 actttttcca gttagcaaat ttttttctga ataaagccaa tccaacaata gcacttatgg
     7801 caatatcagg ttctgatgac aatgcaatat cataaaaaaa cggaattaat gactcatcgg
     7861 gcattgtaag caatgctgtg gttatggtat actgtaatga tttctgctca tgaaaatcat
     7921 taatacatcg gtcacgaaga ccaagcagag aatgcctcca tagtataagc acatcatcat
     7981 gaattaaatt attgataagt aaaaactgct cataatactg taaccctttc atgaaggcca
     8041 gataagtttc ggcttttcta tagagcatgt ctaaaatata tctgtcgcta ttatgaggaa
     8101 actcctggat ttcatatgcc aattgatcaa taaagtggta tagtgctatg gtgataagag
     8161 tatcatagtt aaaccaggaa agaactttat ctctgttatc ataaaataac tgtaatccat
     8221 taatttttgt atcggtatta tggaactgat caatcaaatg atcatagctg aactgtttgt
     8281 catacacatt atgcatagtc agacactatc caaactgggc ttctgtgaat ggaaccataa
     8341 tgaagcagcg ttatgtcgtg tctactattt tttttattta atatgttcta aataatgctt
     8401 tcaatacacc taaaatggta acagtatcag ttgttatggg gttgtatttt tcattagcag
     8461 gtaaaagctg tactttacgc tctgtttttt tgaatatttt cagtgttgct tcattatcaa
     8521 tgagtgccgc cactatttct ccattgttag cacttggttg ctgcctgata atggcaatat
     8581 ctccatcata aataccagca cctgtcatcg agtcaccttt aactttaaga ccaaataatg
     8641 tgccttcggg caaaaatcct gatggaagct gaatataacg ttcaatattt tcctgtgcta
     8701 atatgggtat acctgcagca attgtcccaa ccagtgggat ggaacgaaca tcagcatata
     8761 catcttcttt atcaatgagt aactcaattg cacgcgactt acctgcttca catctcaaat
     8821 atcctttttt ttctattgcc tttaaatggt cataggcacc ttttactgta attgaaaact
     8881 gattgccaat ttcccgaact gttggaggga aacccgtttt tttaatcgtt tgttttataa
     8941 aggaaaaaat tgcctgctgt ttttttgtta aaggttttat catgatacta ccttgccttt
     9001 tttgttaata tatactaatg tatagttgtt gtcaattatt ttttcatcaa ccgcttgctg
     9061 ccatatcatt attataattg tatggaatgc acatgcaggc aaaaacgcta atggtgctgt
     9121 atacgtattt accattgatt gtacacaaat agtttttttt gcaagcacat tttatatcat
     9181 taactatcgc ttaaaaaaaa attcaacttt taatataata catacagtat actttttttt
     9241 gatattcacc ataaaataat tgatttataa aaaaacatag tgcattgcag tgtaaacgta
     9301 tatacttata ttacaatgta aatgctatat aattcacatg ttaacattag aagaatcgta
     9361 tacactattg caggttccaa agaatgcaag tgatgctgaa atagcaaaag cattcaaaaa
     9421 acttgcatta ctgtaccatc cggataaaaa tccccataga attgaatggg ctaacaaagc
     9481 tatggcaaca ataaatgttg cgtacaatac tataatggcc catcgtttca aagacaaatc
     9541 aacagttaat gaaaaagtta cacctcagaa aaaagaacca aaatttaaaa aagaagatat
     9601 actacgcgaa gatcttttaa cacagtattt tatacaatac cgtgaaaaag caaaggatgc
     9661 actctatcaa tatttccagt ataatcttta caatctggca cgcagagaca tgcccgcaaa
     9721 tgctgatata ttcaaaaaaa ttgttgggca gctacgacgc agttaccatg gaatagacaa
     9781 tctgtgtgaa tataccaatg acgaggaatt tttacaacat ttcaatattt tcaaagaatt
     9841 acttttcaca ttttacaaat catcagaatg tctcaatatt attgattcat atgccaacat
     9901 acttgatgtt gaggcattca gaatttacag acagggtgat gactacctgt tgcgttccca
     9961 gaaggaaata ttctatgaaa gacacaacag aggttttttt aaaaaagaac aggctataac
    10021 caatctggtt aaggcaattc agcttttgca gttaacatta gctcgcttcc cacaatcatc
    10081 atgggtagtt gagtcgcaga taaaattaga acatgcgcta agtattgaaa aatatttaaa
    10141 attatttttt gagtgatagc tatggacatt atatccacat tgggaattgc agtggcactt
    10201 gcaatggatg cgttctcagt ttctattgct tcaggattat atatctacaa gccctaccat
    10261 aaattctatt tcagaattgc cttccatttt gggttatttc agtttgcaat gccaattatt
    10321 ggctactttg gcggagttgc catagaacca atcataaaaa attttgacca ctggattgca
    10381 tttggcctgc ttttgttcat tggaatacgc atgataatag cttcgtttag tcaggaaaaa
    10441 gcttcattca agggtgatcc atcaaaaggc tttaccctga tattgttagc acttgcaacc
    10501 agcattgatg cattggcagt gggacttagt tttggcgttc ttcataaacc catcatcatg
    10561 ccaagtatta ttattggtat aacctgcgca ttgttttcta ccttgggagt ttttttgggc
    10621 cgcaaagcaa gtgacattat tggcaaacgc gctgagctaa ttggcggatg catactcata
    10681 atcataggtc taagaatact ctttgcacat atgcaataat ctgttcacgc aacaacacct
    10741 tcgttccgat aatcatactg tactattttt tgggcgatag ttcattatag cagtctgtat
    10801 atgataatta aattacacat actaccatca tgaagataca aacatatatt ataaccatag
    10861 tacttatatt aatagctgta caaactggtg ccgaagatta ccagtatcag tacttccaga
    10921 aagagaaaga agagtttgtc ccattttcag attatatccc taaagtgcgt atgcattata
    10981 aagttgtacc tcattatgtt gaagatttct atgaactgta tggcatgaaa ttgtactatg
    11041 atgaaaactc attgcggaaa aatattgatc gcttaaaaat agcacttacc tgtaaatttc
    11101 gccatccatc gcaagctctg gttaaaatcc aaaccgaaga tgaatattac aaataccgta
    11161 atttgatgtt catgcacatc aacatgttga tactgcgtaa ctatctcaaa attgctacac
    11221 gctatgataa acagcatata tatttttacg atgcagatta tgcaaaagac attcaggaaa
    11281 gcctgaaaat tgcagaacaa atgtataaag aagctttgcc ctactggtat gaagcaaaaa
    11341 aatatgctga cactgcaagt actgtaaaaa ttacaaccaa tttaggattt attgaaactg
    11401 aacgtgtaaa gataatccgt ggcgaagttg attttgaaaa aataattaat gaccacatta
    11461 cccgcgttaa aaaaaagcaa aataatattg acgcattact tgcctcatat cagaagtagt
    11521 atcatggaag taattgaagc cataaaaagc agacgttcaa tacgccatta tacatctaaa
    11581 ccagtttctg atgatgttgt tgtccaaatt atagaagcag gaatatgggc accatcaggc
    11641 cttaacaatc agccatggcg ttttgttatt gttcaatcaa aggataagaa agaagcattg
    11701 tcacaatgca catcgtatgc tcgtattatt aaagaatcac aggtgtgtat atgtgttttt
    11761 tatcacatcc catcaggata caaccgcgat aaagatatac tggggattgg agcatgcata
    11821 cagaatatgc tactggctgc tcatgacaaa ggattggggg ctgtgtggtt gggtgaaata
    11881 ctcaataaaa agcaacaggt taatgaattg tgctctcttt caagcgacta cgaacttttg
    11941 gcagtaattg cattgggata ccccaatgaa aagggaagtt ctacacgcca tccgttagaa
    12001 tatttcatta taaaaaaaat atagtaaata cttacctaca gccatttatt aatactgttt
    12061 attttatcta tagcatcatc aacccattca ttatatggat attcttttat aatttttgaa
    12121 aacagggctt tggcttcttt tagctttact acaagatagc ctttgttacc ggtaaatcct
    12181 gtaccatccg tataatagta ataaaatgcc tgttgcaaaa tagtaaaagc ctgtttataa
    12241 acagcataaa cactgtcctt accattatta ttctttattt cattttgttt gtttagaaaa
    12301 acagcatgtt gtttaagata atcatatgca gtattaataa gctgcatttt ttgcaatgca
    12361 tcggtatccc tgctaatatc gggatgatat aacatagcta acgttcggta agcactggaa
    12421 agctcttcaa gagtaaattg tgattccaat ccaaagatag ttatgtattt tttaaataaa
    12481 tccatacaat tacattatga ataatcaggt gtatgccttt ttgttctttt gttcatacat
    12541 ctgttttcag aaatacagag agtcattata cactgaacaa aaaatgaatg atatcgccat
    12601 catgaacaat gtagtcacgc ccttcacttc gcagcaatcc atgttctttt atagcatgca
    12661 aagatttgta attcataagg tcatcgtaat gaaacacttc agcacggata aagccttttt
    12721 caaaatcgct gtgaataatg ccgctggctt tttgtgcggt tgtcccatgc ttaatagtcc
    12781 atgcctgtaa ttgtgttgta atagtataat aggtgattag gtccaacata gagtacgcag
    12841 ttgttataag gcgttccaat gctgatctgt ccacacccag tgcctggcgg tactcacgct
    12901 gctcatctgg tggtaattca acaatttctt cttcaatctt tgcgcttatt gcaactattc
    12961 ccaacccatt cttttgggca tagtcatgta atgttgtata tacatcagtt gcatttccct
    13021 gctcatccac attggcacaa ataatcattg gcttggcggt aataagtcca tattcattgg
    13081 acactaaaag ctcatctgaa tccagcttga tcctgtttag catgttgccc tgattaatat
    13141 gctgaaataa tctctcaatg acttccagcc tgtgggcact ttctttatca cctgatttag
    13201 cctttttttc aattttttct tttgctctca gtattacctc cacatctgat agcaaaagtt
    13261 ctgtattaat gatttcaata tcacgcaatg gctgaatttc cccctcaaca tggacaacat
    13321 cactgttggt aaaacatctt accacatgca ctatcgcatc aactgcccgt atatggctta
    13381 aaaacatatt gcccagtccc tcaccttttg aagcaccttt aacaagccct gcaacatcaa
    13441 caaattcaat tcttgtagga ataggatcac gtttttccaa tatatttgca attatatgca
    13501 accgttcgtc aggaacaggc acaatgcctt tattaggttc tatagtacaa aacggatagt
    13561 tttccattgg ggcatgtgcc cctgttaatg cattgaatat tgttgatttc ccaacatttg
    13621 gcaaacctat aatcccacag gtaaatccca tgacaatccc cttttaaaac aaaaattacg
    13681 tgaaatatgt tgacatatca ataattgaat accgtgatgt atgtaacatg gaacattgta
    13741 tggcaatgct acaggatatt taaaaaaaat caaatagtat tatggctgaa acaattttat
    13801 tatcattaac actgctatcc tttcttttcc tttgtgtagc aattgctaac gttaatttta
    13861 aagaagggcg ttactctgta cccattgtat tatgggtaac agcatcgggt gcgatagctc
    13921 ttgtaaatgt atcccataca tacatcacct taattatacc agtatacccg tttgtttttg
    13981 cactgtttat tcacgcttta atcaaaccat taagtcgtgg tgatatctat acatatggaa
    14041 tttctcagct tgcaactatc attgctttat ttttgccatt taccattatg cctgtaatcc
    14101 tggcattcat tcatatatat actgccttta caatacataa aacaatatca ttcaaatatg
    14161 gcataaaatt cttacagggg atgctgatca tcgaatcggt cattttaggt tttatatctt
    14221 atatgcaaaa catacaggta cttatcatta ctcttacagg tttcattaca gtttttttaa
    14281 ctatcatgat atatgataca ttgaaacgtt ctcaaactgc actcttaaaa atacgggcac
    14341 ttgcgcgtat gaatgcaaac ctgacaaagt taaacaggct tttaaagcaa agaaatgacc
    14401 agtacattgc aatgctcaat aagaaagaca atgatatcca gttactttca aaatactcaa
    14461 ccatggccga aattactgca agcatagccc atgaaattgc ccaaccctta acaggcatca
    14521 agagcattgc ccagaacatg atggatgata ttaatcttga tgattttaat tatttagaag
    14581 ctttatctga acttcagaag atttgcacca tggttgacaa agcaacctat atcattgatc
    14641 acatacgtag ttttaccact aacaaccaca ttttcaaact aatagatatt aacacaataa
    14701 taatgaatgc agttacactt acccacaatc aattgaaaaa gcataatatt gatatagtac
    14761 ttttgctgaa tgattcgata ccaaaaattt atggaaattc aataaacata gaacaaatac
    14821 tgttgaatct tattaccaat gcacgtgatg ccatcattga aaaagcaaaa aaaatgcaat
    14881 ctgattacac aggaaaaatt actattaaaa cagattattc tgatgactat gtaattgtca
    14941 taatcgaaga taatgggatt ggaatccagc ctgacataca aaaacacata tggtccccat
    15001 ttttcacaac aaaaaaatat agtcagagtt taggcatagg attgtcaatt agcaaaaaaa
    15061 ttatcaatga ccataatggc gaaatttcaa tagactcaac tccagatgaa ggaactatat
    15121 tcatcattaa attttcttta ctaaaataaa aaatattctt tctgtattta aaatttttaa
    15181 attgaccagc ccaataattt aattatacta tttttcacta tcacacgttc atttgggtgg
    15241 gggtttgcat gttatatcca tataaattaa tactatttat tatgtttgtt tttttgatta
    15301 cttttataca actaaacgct actgcacttg aagttgctat tgacgaaata cggacagaac
    15361 ctgttatttt tgtaaattat cagggcgcat ataagaaagc agattcatta caggacatag
    15421 aaaatattgg gaaatcactg gcacgcaaca gtaacaatac tggaattatc cgttttggaa
    15481 tgaaatattc attaataaat gcaatttcag aagaggaacc aaaaaaattt tcagctacaa
    15541 tattttcaat agataaaaag gcaaaggttg gacatattga tataatccgc aaaatcctgt
    15601 cagcctattt acagcaaaga tatggttact cacaacaaca cgcaaaagct atcgccctat
    15661 ttttaacata ttataatgct atatacaggg ggaacattga ttacttttca tcaaaataca
    15721 agagcattgt tataaaatat ataacaaaat ataatgcagg catttcaaca aaatattacg
    15781 aatggccagg agcaacaaaa atgctcatcc ccttgaccga acaaccacaa aaaggaaagc
    15841 ttgattctat cctgatagat attatatcag ataaaaaaat actacaagaa ataagaaaag
    15901 atgataaaaa cattcaggca cgtaaggaca tggtggacat aaaggaaaaa atcttaacac
    15961 aggaaaaagt agatcttgag aaaagtaaaa aagatgtgat tcaggaaact actacattag
    16021 aagaaaataa aaaatccatt gaacagaaaa aagaggaact acagcaaaca gaaaaagaaa
    16081 taaataaaat gaaggaagaa ataaaaaaag aaccatcccc agaaaaacaa aaggacatcc
    16141 agaaagatat tgaaacaaaa gaacaacaac tacaaaaaca aaaggaagat ataaaaaaga
    16201 aagaacagga gatagcaaaa aaggaatcag aaattttaga taaaaagaat aaagtttcag
    16261 aaaaagaaga acgtttagct gaaaaggaaa aagcacttga aaaagaaaag gaagaaatag
    16321 ctgctgatga aattaaaaaa gcacccccaa aagaaaaaga attagttaaa aagctagaaa
    16381 aaaaagaaaa agaattggat gaaagagaag ataaactgcg atcaaaagaa ctggatcaaa
    16441 atatttatgc tgataaactg tattatctga aaataaagga atacctgcag gatggtcatt
    16501 ataacaacga aatggttatg atagatcctg catcccgcaa aattttattt aaatcaccag
    16561 aaaaaaacat ctgtggtagc cgatatgata tattctcagg cggtgttgtt gtcattacac
    16621 ataagggcag ccatgcaata gggcaccggc ttacattact tgacaggaat agtctggaag
    16681 ccaaattata tggttccgat aacattttct ggcgtagctt tataataatt aaggatggat
    16741 ttatttatgc gatagtaatt gaaaatggca aattttatct tggccgcttt gatacaaatc
    16801 tcaaaatggt ttcaaaatcg tcagaagagg ttaatgagaa cacatttatc agcttttggg
    16861 ataattttat atatataaat agagtggata aaactattat ggtgcttgat aaagaaaact
    16921 taacactaat tgatacgatt aaaccataaa atctaaaatt ttgttaatat ataataaaaa
    16981 agcccctctc attacaaaaa gaggggcttt ttataatatg gtcaagcctc acgactgatt
    17041 agtactggtt ggctgaacac attgctgtgc ttacacctcc agcctatcaa cctcgtaatc
    17101 ttcgaggagt cttcagtgac ctcaagggtc aggggaaatc taatcttggg gttggcttcc
    17161 cgcttagatg ctttcagcgg ttatccgttc cgaacatagc tacccagcgg tgccgttggc
    17221 acgacagctg gtacaccaga ggttcgtcca ttccggtcct ctcgtactag gaacagatcc
    17281 cctcaaattt ccaacgcctg cagaggatag agaccgaact gtctcacgac gttctgaacc
    17341 cagctcgcgt tccgctttaa tcggcgaaca gccgaaccct tgggaccttc tccagcccca
    17401 ggatgcgaag agccgacatc gaggtgccaa acagtgccgt cgatatgaac tcttgggcac
    17461 tatcagcctg ttatccccgg ggtacctttt atccgttgag cgatggccct tccacgcggg
    17521 accaccggat cactaacccc tactttcgta tctgctcgac ttgtaggtct cgcagttaag
    17581 ctcccttatg cgtttgcgct ctatgcacga tttccgtccg tgctgaggga acctttgggc
    17641 gcctccgtta ctctttggga ggcgaccgcc ccagtcaaac tgcccaccag acactgttct
    17701 gatcctggat caacaggaat cagttagtaa cccgatacga taagggtggt atttcaagga
    17761 cgactccatc ccgactagcg tcgagacttc ccagtctccc acctatccta cacataccgc
    17821 accaaattac aatgtcagga tgcagtaaag gtccacgggg tctttccgtc cttctgcagg
    17881 tagtctgcat cttcacaaac attacaattt caccgtgtcc ctcgttgaga cagtgaccaa
    17941 gtcgttacac cattcgtgca ggtcggaact tacccgacaa ggaatttcgc t
//
LOCUS       DAORYL010000062        17873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_2588_length_17873_cov_14.273880, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000062 DAORYL010000000
VERSION     DAORYL010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 17873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17873
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2588_length_17873_cov_14.273880"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..1167
                     /locus_tag="PLV81_11020"
     CDS             229..1167
                     /locus_tag="PLV81_11020"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOM10668.1"
                     /translation="MKKVKENITGVVTVLLLVLNTLFWASFLFPIAVLKIIPVKIWRK
                     FINNILDTIACCWIYCNNAGLKMTRTIHWDVSGVENLSKKSWYIVIANHQSWTDIVVL
                     QKILTGKIPFLKFFLKKELIWVPVLGLAWWALDFPFMRRYSQSFLLKYPHLKGKDIEV
                     TKKACQKFKYIPVSIMNFVEGTRFTPEKHAKQESPYKNLLKPKSGGIGFVFSTMGNMI
                     TSILDVTIVYPQGRMSFWDFLCGRVNVIKVHVEEIPVTDDFIGDYVDDPVFKNKFQQW
                     LNDLWYEKDIRIDMLKGKSLPLSAKPYAEDEAMIIN"
     gene            complement(1231..2325)
                     /locus_tag="PLV81_11025"
     CDS             complement(1231..2325)
                     /locus_tag="PLV81_11025"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOM10669.1"
                     /translation="MKHLDEFTLFALAKNSLYGDDAIDATLHIKQCYICSKKLQLIQA
                     TIAPQKIVEPSRDILTSILEYHSESVSQKESFFNTIFNFIQTYKRSLAFTTGIVILGV
                     ALLLSIKPNYKSIPHILYIAGKASNTVTTQTIKEGHRIILNESDSAVLVANNDITFRL
                     AGTVDLTISKSRYNTALDKKRFQYILSNGVANIKSYSSHETMQYEIKTPDAIIQPLGT
                     EFYINVSPEGTHIHIVEGNVVVNNTATGQTINGEIGKLYTINKKEISVFDIEKYQLQW
                     VNDIDMSFKDLLDNPDFGEYSAINSNINDNTGNESSTETKPENIIVPDNPDNNEETES
                     LKNRNDIQDIKEFQNEIRQMKKESKHKHRE"
     gene            complement(2448..2960)
                     /locus_tag="PLV81_11030"
     CDS             complement(2448..2960)
                     /locus_tag="PLV81_11030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOM10670.1"
                     /translation="MISREELISLYSTHYKEIFNYIYHLTGSHETAEDILQETFIKII
                     EYSKNNIINLDTVKSLLYRTAHNLSINYLKKNEKNDSLDDDINYFPAASTVDDDLFAK
                     EIQNEVSNALQQLDTVSRSIFIMKRDNNYTNEEIAKLLQISERTVRRKLQSTLSHILS
                     HLKTKGYFEE"
     gene            3080..3400
                     /locus_tag="PLV81_11035"
     CDS             3080..3400
                     /locus_tag="PLV81_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10671.1"
                     /translation="MKKVLATLVSLLFAAAVFAQTQLQTKEATKNVGQEKKIEVQQKA
                     EVKKQEQTQKKEAKKEEKAKAKEEKKQEKEQKKEAKKEAKQEKKEAKEQKKEAKQEQK
                     GAAK"
     gene            3642..4556
                     /locus_tag="PLV81_11040"
     CDS             3642..4556
                     /locus_tag="PLV81_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10672.1"
                     /translation="MSCHKRLYFIIIFLTVLIITCHPITVFAEKIKTTIGFKSIYSSG
                     ISDTNIAYKPFGRIAYDINFFKLEATGMYTINQQITDGMGNFTEINTCQGQVKTLLTI
                     GEFMDLGGGYSLTKGENSYTSKMYMANGSIYIGDATIDLDYSRENKEYSYNGAIDIIN
                     QIFIGSLSYDINDFIGYELEYHYLANNFSNLDYTYDKKIVRIGMSVYGDKTVYMAGIN
                     AGTDSGDYTIFGADFGLSKKFFDAIKILLAYNVDYYNPPSVTSTSGGGHGGSNGGGYK
                     GMNPYLRSSLAGKSFFSHSLSASISYLF"
     gene            4635..5528
                     /gene="nadA"
                     /locus_tag="PLV81_11045"
     CDS             4635..5528
                     /gene="nadA"
                     /locus_tag="PLV81_11045"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOM10673.1"
                     /translation="MIKEIEKLKKQKKAIILAHNYQRPEVQDIADFTGDSLELSRIAA
                     DNDADLILFCGVHFMAESAYILSPQKKVILPDLSAGCPMADMAEAEDVLKMKEENPDA
                     AVVTYINSSAEVKAVSDICVTSANAVTIVNKLQNDTVLFIPDKNLANYVQRFTSKKII
                     PWKGFCPTHDRFTKEELIAIKQKYPEACVIVHPECRPEIIDMADNVWSTGQMVKNIHT
                     IPHNQIIVGTEEGMLHKLKKIAPEKEFILASKGFMCPNMKKITLEKILKALQDEQPIV
                     TVKEDTRIKAFMALQKMLELS"
     gene            5547..6626
                     /locus_tag="PLV81_11050"
     CDS             5547..6626
                     /locus_tag="PLV81_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10674.1"
                     /translation="MENIEFSDEIQKIREKTFNTEFKELVAKVIYKPQDSIDNPDSLL
                     NSLHKLYYTLLSRSFNDAITFVNPEIIYENTLQFLGEELNKKLKSLGFYEALIFQYDF
                     KQNYFGILINSFNDIIFRDFFITLSDDLYKKIISQPEGVIIDINSLKNDKLYEKRLKL
                     FEDQSSLIWAFSTFPMVKKVISFLNIPCPTAIEIFCPVIFVRVHKEFTINQKIINELY
                     YNCIYTIFSLIELSLAINKGSLFLSSNYFHEVFKVFENYYYIAKSNDYVKYVIIKSKY
                     EDQYIYCLFRYLSNKLNKLKSYSCINYRPMIGLIVLFLSEENKNIESELEVISTLFGD
                     SIKIGQIDNSKIKNAVNLYKYIIDL"
     gene            6917..7774
                     /locus_tag="PLV81_11055"
     CDS             6917..7774
                     /locus_tag="PLV81_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10675.1"
                     /translation="MLVKIEQIKKVLENNPACVCKYLQSYTINGKTYHESDQIFIDDI
                     YRFEQAGVETIEIKYDEIVYSLLSTLYPAEYRVPYASADFITIDRKLETLDRVSTLTK
                     RKRYLICIGDIYSYDQHTGKRITVFKHNDPIDYKQWNQVKRLLDRNKRIYYRNSENGI
                     IIFVNLQPHAETSYIERFKKNTDLVSAIVSRKKDCRIEISPDFLPTEDVFTVNDPREL
                     LKFYQQSNARLIIIGETLNDDYRKALLQVREYDKFARMMVVPIIDLRNIDHFLLQVKM
                     VYNADRWSE"
     gene            7794..8714
                     /locus_tag="PLV81_11060"
     CDS             7794..8714
                     /locus_tag="PLV81_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10676.1"
                     /translation="MRKPKKKSILKYFEPIDSGKTLSENFGSLDLEGSFGYFALSSQN
                     KNLFLNRLDVDTLYGMIKKVGLVDHLLGLGFNNIQIDIDKDNAQIHYLKIYYEKMDPE
                     HLLIDLRVSESRFMPQKRFCEEYDAPTSLDMVVIEWLSAQNPKATFDPNKPQLPGQSK
                     PGLGSLNYMMELMYIVGEQVVKDGFMDVPDHFHGAVMYSRKFKFFNPSHEAILRAILR
                     DIKGYSLSDMSWGMITGTIIDKNTNNPQVYDPSEQIFPVSKRMKEYFESKKYKVKFKK
                     IYESKKYYFDYEKMCQIREKMLHENKTDEL"
     gene            complement(8719..9894)
                     /locus_tag="PLV81_11065"
     CDS             complement(8719..9894)
                     /locus_tag="PLV81_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007642262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HOM10677.1"
                     /translation="MLERTKIPRNDSNDYTLEMAKRRQNFITTMTGTELHHVSSFSFD
                     PKVLPGNIEHFTGVAQVPIGIAGPLLIHGEHANGEFYVPLATTEGTLVASYNRGMKLF
                     YEAGGITTTVSDDSMQRAPVFVFENAIFARNFGEWIDEHFYDIKEQAESTTKTGKLKK
                     IEKFHVGNMMFLRFNFSTGDAAGQNMVGKATWVACNWIMKNNPTIKRYTLSGNIDTDK
                     KYSHLNTLSTRGKRVTAEITIPRKLFIDFMKLPPEAVVKMRYTTTLGAFISGANNNGL
                     HSANAITAIFIATGQDVANIAESSSAIAYAEVTENGDYYYFITLPSLIIATYGGGTGL
                     PTQKECLEILGCYGAGKVYKFAEIIAATVLCGEFSLATAVISGGEWVSSHEKLGRNR"
     gene            complement(10112..11788)
                     /locus_tag="PLV81_11070"
     CDS             complement(10112..11788)
                     /locus_tag="PLV81_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOM10678.1"
                     /translation="MSKKSISYKDKPWLKFYDKGVPSTINFVDCTLNDYFDEAINKYA
                     DRVAFVSQGYEMTYRELGDAVNKFATCLADFGINKGDRVAIHLPNILHCVAAYYAVIK
                     IGGIVVMHNPLYSPSELEYQFNDSGAKVVVTLDLFGNTIIDLRSKTQVKQIVCVSLRD
                     YLPSDVDPNTILSVEPKQAEDVYKWKDIINKYPAQPPKVTVTTEDIAQLQYTGGTTGV
                     SKGAMLTHSNLTKQLQQIDAWDTTIHRGDAMTMVGALPFFHVYGLSTVMNLNIYDGFK
                     TILTGRPTPESLIEIIQKYRPQIACLVPTMYIGMLNHPDFEKLDLTCIKRLMCGSAPL
                     PLEVIKAYEEKAGVHINEGYGLTEASPVTHSNPYGTVQKSGSIGIPYPSTEVRIVDLE
                     TGENDVPVGQPGEMIIKGPQIMKGYWNRPDETAKTIRNGWLYTGDIAIMDNDGYFFIV
                     DRKKDMVISGGYNVYPREIDEVLYTHPKVLEACCIGIPHPTRGEQIKAFVVLKEGETA
                     TEKEIIDYCATKLAKYKLPTIVEFRTELPKSNVGKILRKVLREEEMQKLK"
     gene            complement(11926..12423)
                     /locus_tag="PLV81_11075"
     CDS             complement(11926..12423)
                     /locus_tag="PLV81_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10679.1"
                     /translation="MAKLDKNRLIFGLLLIILIAIGEIVFHVLHVPAWPAFLIMIFFF
                     MAHMDKKLAPNMLVGGAFGILMIIAAKIIVTSLVGAGLDQFVAFLLFVLVFVYCIVAL
                     GEILPVLFNNYAFMALLFTATYMQTPNPQNPFVLTAILLIGGGIFILGILGIVKLLMK
                     LASSK"
     gene            complement(12834..13226)
                     /locus_tag="PLV81_11080"
     CDS             complement(12834..13226)
                     /locus_tag="PLV81_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10680.1"
                     /translation="MLLKKFEVNLQTIQINLINENEEAIVTIISSNNEIIEDISLCNY
                     SIASCINDITSKIGIEYTDFENFLKKINHPVANIISDTMKNINSAITYIYNLNQTLII
                     LLSNHEESIVKNIHEINKIIELQTQLAY"
     gene            complement(13276..13710)
                     /gene="fliS"
                     /locus_tag="PLV81_11085"
     CDS             complement(13276..13710)
                     /gene="fliS"
                     /locus_tag="PLV81_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002176736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HOM10681.1"
                     /translation="MALPSRNPYDQYKHTEINTANQGKLIVMLYDGAIKFCNIAIEHM
                     HPKTYDIANTNILKAQDIINELILALNMNEGGDVARNLFNLYIFFKKQLLEANIQKNA
                     DILKNVVKLLRELRDAWDKISTKETPSDRVATASKGSFSIEG"
     gene            complement(13806..14690)
                     /locus_tag="PLV81_11090"
     CDS             complement(13806..14690)
                     /locus_tag="PLV81_11090"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOM10682.1"
                     /translation="MEAVTTTKVPGTRKLFSGKVRDVYKVDDEHLIIVSTDRVSAFDW
                     VFPNGIPGKGIILNTISNLWFKNIKFINNHIVETNYKNFPKPFCDYPEFFQDRAVLVK
                     KAKRIDFECVARGYIIGSGWKDYQKTGKVCGIELPKGLQLASELPYPIFTPATKAASG
                     HDENITIEEMKKALGTDVAEKLAQATLDLYDFARDKLKKAGIILADTKFEFGFSEKEI
                     ILIDEVLTPDSSRFWDAALYKPGISPVSLDKQFIRDYLETTTWDKNSPPPPLPDEIIQ
                     KTKEKYEEILQRIKHILG"
     gene            complement(14741..16174)
                     /gene="gatB"
                     /locus_tag="PLV81_11095"
     CDS             complement(14741..16174)
                     /gene="gatB"
                     /locus_tag="PLV81_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOM10683.1"
                     /translation="MEFDAVIGLEVHVQLNTQSKIFCSCSTQFGSQANTQVCPVCLGL
                     PGVLPVLNKEVLRKAIQAGLALNCKIATYSKFDRKNYFYPDLPKAYQISQYDKPICYD
                     GFIEIVTDNGIKKIGITRLHMEEDAGKNIHSEDSDYKVSYVDFNRTGVPLIEIVSEPD
                     INTPDEAYQYLQNLRSILKYIEVSDCNMEEGSLRCDVNVSLKPKGSTTFGQKVEIKNL
                     NSFRSVKLALEYEIERQKKMLLQNEKIVQETRLWDADRNITFSMRSKEEAHDYRYFPE
                     PDLPPIEISREFIEQLKKELPELPHQKRIRFMQQYGLPEYDAQVLTSTRQLASYYEAV
                     VADGAQPKKASNWIMSEVLAKIDDPETIDSFIVKPKDLAILLKRIDDATISGKIAKTV
                     FEEMLETGKNPDTIINEKGLRQVTDTGAIESIIDEVIKNNPKSVEDYKKGKDKALKFL
                     IGQVMKESKGKANPQMVNDILIKKLSN"
     gene            16603..17148
                     /locus_tag="PLV81_11100"
     CDS             16603..17148
                     /locus_tag="PLV81_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10684.1"
                     /translation="MYGPVLSVNVMDNWSISGVFLYGEFKNPEYSRGTADFGNFKRYD
                     FDGTINYGLTNFLKIFIGGKYVKFDFLVSNQDASYYMYGPGAGASVIFNIVDNLYFTA
                     NTSYVYMKGKAELVDSSSSMITKGINSSASLGYYFASTSVTVYIGGRYQRLFEDRKVE
                     DSFKYHEMMGVTAAATYSFML"
     gene            17274..>17873
                     /locus_tag="PLV81_11105"
     CDS             17274..>17873
                     /locus_tag="PLV81_11105"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOM10685.1"
                     /translation="MKKIRILSTNTVMGIILFTYILALIPSRALPQTIDFISAQSKLL
                     ENNPTIKAMQLRVEQYKKKITVTSSLPDPTIKLGLVNIPVTKLSLNQSDMTGKEIGFY
                     QMFPSFGTLSTKERIALLEYKKAIENERFYTAYYLHTLRTYFFEIAYLHEYLKIIEET
                     KQYLTVLLDVQKAKSSTGAGSLSDILKISVEMAKLDEEII"
BASE COUNT         6042 a         2706 c         2978 g         6147 t
ORIGIN      
        1 cctcacagtt caaaattatt tctttttgaa aaataatttt gcaaccgttt actgattacc
       61 ggtgtctttt taatgggttg ttggtttgta taaatcagtc agtaaataaa aaatcctatt
      121 tatccattat tgcaccggtg caggattgat agtaaacgta atagagtaat agattccaag
      181 tcaggttcag aataacgatg aagaaattaa attttcagga gaagattaat gaaaaaagtt
      241 aaagaaaaca tcactggagt tgttactgta cttttactgg tactcaatac attattctgg
      301 gcttcatttt tatttccaat tgcagtttta aaaataatac ctgtaaaaat atggcgtaag
      361 tttataaata acatactgga tactattgca tgttgctgga tatactgtaa taacgctggt
      421 ttaaaaatga cgcgtaccat acactgggat gtttctggcg tagaaaatct cagcaagaaa
      481 agctggtata tagttattgc caatcatcag tcatggacag atattgttgt actgcaaaaa
      541 attttgactg gcaaaatacc atttttaaag ttctttttaa aaaaagaatt aatatgggta
      601 ccagttttag ggttagcgtg gtgggcactg gattttccat ttatgcgacg atattcccaa
      661 tcatttttac tgaaatatcc tcatctaaaa gggaaagata ttgaagttac caaaaaagca
      721 tgccagaaat ttaaatatat tcccgtgtcc attatgaatt ttgtagaagg gacacgattt
      781 acaccggaaa aacatgccaa gcaggaatca ccatataaaa atctcttaaa accaaaatca
      841 gggggtattg ggtttgtatt ctcaactatg ggcaacatga ttacgtccat tctggatgtt
      901 accattgtat acccgcaggg aagaatgtca ttctgggatt ttttatgtgg cagggtaaat
      961 gttatcaagg tgcatgttga ggagattcca gttactgatg attttattgg agattatgtt
     1021 gatgatccag tattcaaaaa taaatttcaa cagtggctca atgacctctg gtatgaaaaa
     1081 gatatcagga ttgatatgct gaaagggaaa tcacttcccc tttcagcaaa accgtatgca
     1141 gaagatgaag caatgataat taactaataa cacactatat ataacattga gtacaattcc
     1201 gctgttttag atgtgggaat attaaaatca ttattccctg tgtttatgtt ttgattcttt
     1261 tttcatctgt cgtatttcgt tctggaattc tttaatgtcc tgaatatcat ttctattttt
     1321 caatgattca gtttcttcat tattatcagg attatcaggc acgataatat tttctggctt
     1381 agtttcagtg gaactttcat tgcctgtatt atcattgata ttggaattaa ttgcggaata
     1441 ttccccaaaa tccggattat ccaataaatc tttaaatgac atatcaatgt cattaaccca
     1501 ctggagttga tatttctcaa tatcaaaaac agagatctcc tttttattta ttgtatataa
     1561 tttcccaatt tcaccattta tggtttggcc agttgctgtg ttatttacga ctacatttcc
     1621 ttcaacaata tgtatatggg ttccttcagg tgatacatta atgtaaaatt ctgtaccaag
     1681 tggctgaata atagcatcag gtgtttttat ttcatactgc atagtttcat gggaactata
     1741 tgattttatg tttgctacac cgttggataa gatatactga aatctctttt tatctaatgc
     1801 agtgttatac ctggactttg aaattgttaa atcaacagta ccagccaacc tgaacgtaat
     1861 gtcattgtta gctaccaaaa ctgctgaatc gctttcattc aatataatcc tgtggccttc
     1921 tttgatggtt tgggtagtga cagtattact ggctttacct gcaatataca gtatatgtgg
     1981 aatagatttg taatttggct taatacttaa taataatgca acacccaaga ttacaatacc
     2041 tgtggtgaaa gccagagacc gtttatatgt ctgaatgaaa ttgaatattg tattaaagaa
     2101 tgattctttt tgtgatactg attctgaatg atattccaga attgaagtta aaatatcacg
     2161 ggatggttca acaatttttt gtggggcgat agtagcctga atcagttgta actttttaga
     2221 acaaatatag cactgcttaa tatgcagtgt tgcatctatt gcatcatctc catatagact
     2281 attttttgcc agagcaaaca atgtgaattc atctaaatgc ttcataacat attcagtacc
     2341 cttatgtata atacaattgt atcacaaatt ttggacacac aatagtataa attttaactc
     2401 attattgaaa aaatattaac cctccagtta gcagtggagt agtatactca ttcttcaaaa
     2461 tatcctttag tttttaaatg ggatagtata tgcgataatg tagattgtaa ctttcgtcgc
     2521 acagttcttt cggatatttg taaaagtttt gctatttctt catttgtata attattatct
     2581 cttttcataa taaatattga acggcttact gtatccagct gttgtagtgc atttgaaact
     2641 tcgttctgaa tttctttggc aaataagtca tcatccactg tactggctgc tggaaagtaa
     2701 ttaatatcat catccaaact atcgtttttt tcattttttt tgagatagtt aatggataga
     2761 ttatgtgctg ttctgtataa aagggatttc acagtatcaa ggtttattat attatttttt
     2821 gaatattcta ttatttttat aaaagtttcc tgtaaaatgt cttcagcagt ttcatgcgaa
     2881 ccagttaaat ggtaaatata gttaaaaatt tctttataat gtgttgaata tagcgatatt
     2941 agttcttcac gggaaatcat atttttttta aaaaatttgt ccgttttttt tcagttggat
     3001 tgtattatac tgcaaagagt tgataaacaa taatttttat caacaaaaaa ttttaaaata
     3061 aactctatca ggaggatgta tgaaaaaggt attagcaaca ttagtatcac tgctttttgc
     3121 agcagcagta tttgcacaga cacagctaca gacaaaagaa gctacaaaga atgttggaca
     3181 ggaaaaaaag attgaagtac agcagaaagc tgaagtaaag aaacaggagc agacacagaa
     3241 gaaagaagct aagaaggaag aaaaagcaaa ggctaaagaa gaaaagaaac aggaaaaaga
     3301 acagaagaaa gaggccaaga aagaagctaa acaggaaaag aaagaagcaa aagaacagaa
     3361 aaaagaagct aaacaagaac agaaaggtgc cgcaaagtaa gaatgtaatt cagtggagtg
     3421 ctattgataa gccatgcata aaaaagcatg gcttatcttg ttaattaata gaataattta
     3481 cattaaattt tccataaaca tagtataaaa aattaaatag tatggtagta aaaactggaa
     3541 gtatatggaa tgcataataa aatatttttt acaaatttat ccgattcgtg ataatgataa
     3601 tttagttagt aaagattcaa actttctaaa aggggaatgt tatgagttgt cataaaaggc
     3661 tatattttat cataatcttt ttaactgttt taattataac atgccatcca attacagtat
     3721 ttgctgagaa aattaaaaca acgataggat tcaaaagtat ttactcttct ggtatttctg
     3781 atacaaatat tgcatataag ccctttggca ggattgcata tgacataaac ttttttaagc
     3841 tagaagcaac aggcatgtat actattaatc agcaaataac agatggtatg ggcaacttta
     3901 ctgaaattaa tacatgccag ggacaggtta aaactttact gacaattggt gaatttatgg
     3961 acttaggcgg gggttattca ctaacaaaag gtgaaaattc atataccagc aaaatgtata
     4021 tggctaatgg cagcatttat attggagatg caaccattga tttagattac agcagagaaa
     4081 ataaggaata cagttataat ggtgctattg atattattaa ccaaatattt attggttcat
     4141 taagttatga tataaatgat ttcattggtt atgaattgga atatcattat ttggccaata
     4201 atttttcaaa tctggattat acctatgata aaaaaattgt aagaataggc atgtctgtat
     4261 atggtgataa aacagtatat atggcaggaa ttaatgctgg aactgattca ggtgattata
     4321 caatctttgg agcagatttt ggtttaagta aaaaattctt tgatgccata aaaatattgt
     4381 tagcctataa tgtagattat tacaatcctc cttctgtaac ctcaacatca ggtgggggac
     4441 atggtggtag caatggtggt ggatataaag gtatgaaccc ctatttgcga tccagtcttg
     4501 caggaaaatc atttttttca cactcattga gtgcttcaat ttcatacttg ttttaataaa
     4561 atttccttga agttttaaat attgcacttt gtgattgtat tgattataga ttctaaattt
     4621 caaggagtgt aggtttgata aaagaaattg agaaattgaa aaagcaaaaa aaagctataa
     4681 tacttgcaca taattaccag agaccagaag tacaggatat tgcagatttt acaggcgatt
     4741 cattggagtt atcgcgcatt gctgcagata acgatgctga cttaatacta ttttgtggtg
     4801 tccatttcat ggcagagtca gcatatatac tatcgccaca aaaaaaagta attttacccg
     4861 atctttcagc aggctgcccc atggcggata tggccgaagc agaagatgtt ttgaagatga
     4921 aagaagaaaa tcctgatgct gcagtggtaa catatattaa ttcttctgct gaagttaaag
     4981 ctgtttctga catttgtgta acatcagcaa atgctgtaac tattgttaat aagttacaaa
     5041 atgatacagt tttatttatt cccgataaaa atctggctaa ttatgttcag cgatttactt
     5101 ccaaaaaaat tatcccctgg aaaggatttt gcccaactca cgatcgtttt acaaaggaag
     5161 agttgattgc tataaaacaa aaatatccag aagcatgtgt gatagttcat cctgaatgca
     5221 ggcccgaaat cattgacatg gccgataacg tgtggtcaac cggacagatg gtaaaaaata
     5281 tacatacaat tccacataac caaattattg ttggaacaga agaaggaatg ctgcataagt
     5341 tgaaaaaaat tgcgccagaa aaagaattta tacttgcttc aaaaggtttt atgtgtccaa
     5401 atatgaaaaa gattactctt gaaaaaattt taaaagcgtt acaggatgaa caaccgatag
     5461 taactgtaaa agaagatacc aggattaaag cttttatggc attacagaaa atgctggaac
     5521 tatcctgatt tgtggtgagg catactttgg aaaatattga attcagtgat gaaattcaaa
     5581 aaatacgtga aaaaacattc aacacagaat ttaaagaact agttgctaaa gtaatttata
     5641 aaccccagga ttctattgat aaccctgata gcttgcttaa ttccctccat aagttatact
     5701 atactctttt aagcagatct tttaatgatg caataacatt tgttaatcct gaaataatat
     5761 atgaaaatac attgcaattt ttaggagaag aattaaataa aaaacttaaa tctttaggat
     5821 tttatgaggc tttaatattc cagtatgatt ttaagcaaaa ttactttggg attttgatca
     5881 attcatttaa tgatatcatt ttcagggatt tttttataac cttatcagat gatctgtata
     5941 aaaaaattat cagccaacca gaaggtgtaa taatagatat caattcttta aaaaatgata
     6001 aattatatga aaaacggttg aagttatttg aagatcagag ttctttaata tgggcatttt
     6061 caacatttcc tatggttaaa aaagttatat cttttttaaa tatcccatgt ccaacagcta
     6121 tagaaatatt ctgcccagtg atatttgtca gagtacacaa agaatttaca attaatcaaa
     6181 aaataattaa tgaattatat tacaattgta tctatacaat tttttcttta attgaattat
     6241 ctttagccat aaataaagga tctctttttt tgagctcaaa ttattttcat gaagttttta
     6301 aagtgtttga aaattattat tatattgcca aatctaatga ttatgtaaaa tatgtgatta
     6361 ttaaatcaaa atatgaggat cagtacattt attgtctttt caggtacctg tcaaataaat
     6421 taaataagtt aaaatcttat tcatgcataa actacaggcc aatgataggt ttaattgtat
     6481 tatttttatc tgaggaaaat aaaaatatag aaagtgagct tgaagttata tccacacttt
     6541 ttggtgattc aataaaaatt gggcagatag ataactcaaa aattaaaaat gctgtaaatt
     6601 tatataaata tattatcgat ttataaaaga agttattctt aacatattac gtatgtaaag
     6661 attaaaatca ttcatcagca agtgatacta ttgtgcattc tttggtattt tgttaataat
     6721 tgactggtta gatgtataca ataatttaga actataaata atgtaaatga aatttaatta
     6781 aatttttatt gaataaataa aatatacttg aataaaagaa tattcagtta ttatacttat
     6841 tttaataggg tatttaaccc aatattaaaa tattgttttg attgtattta aaatgtaatt
     6901 atgttaaatt gagacaatgc tggtaaaaat tgaacagata aaaaaagttt tggaaaataa
     6961 tcccgcttgt gtttgtaaat atttgcaaag ttatacaata aatgggaaaa cctatcatga
     7021 atcagatcaa atatttattg atgatattta cagatttgag caggctggtg ttgaaacaat
     7081 cgaaattaaa tatgatgaaa tagtttatag tttattatcc acattatatc ctgctgagta
     7141 tcgtgtaccg tacgcttcag ctgattttat tactattgac cgtaagctgg agacacttga
     7201 tagagttagc acattgacaa agcgaaaacg ttatttgatt tgtattggag atatttattc
     7261 gtatgaccag catacaggga aaagaattac ggttttcaaa cataacgatc caattgatta
     7321 taaacaatgg aatcaggtaa aaagactttt agataggaac aaacggatat attatcgtaa
     7381 ttcagaaaat ggaataatta tatttgtaaa tctacagccg catgctgaaa ccagctatat
     7441 tgagagattt aaaaaaaata ctgacctggt aagtgcgata gtttcacgta aaaaagattg
     7501 cagaattgaa atttcacctg actttcttcc cactgaagat gtttttacag taaatgatcc
     7561 aagggaactt ttaaagtttt atcaacaatc taatgcacga cttataatta ttggtgaaac
     7621 attaaatgat gattatagaa aagcattatt acaggtccgt gaatatgata aatttgcacg
     7681 aatgatggtt gtgccaataa ttgacttgag gaatatagat cattttttat tacaggtaaa
     7741 gatggtatac aatgcagata gatggtcaga ataaacgaaa ggagaaaatt tgagtgagaa
     7801 aacctaaaaa aaaatctata ttaaaatatt ttgaaccaat cgattctggg aaaacacttt
     7861 cagaaaattt tggatcgctg gatcttgaag ggagttttgg atattttgcg ttgagcagtc
     7921 aaaataaaaa tttattttta aacagattgg atgtagatac tctttatgga atgattaaaa
     7981 aggttggcct ggttgaccat ctactaggat taggatttaa taacattcaa attgatatag
     8041 ataaagacaa tgctcaaata cattatttaa aaatatatta tgaaaagatg gatcctgaac
     8101 atctcttaat agatttaaga gtttctgaat ctcgttttat gccacaaaag aggttctgtg
     8161 aggaatatga tgctccaacg tcattggata tggttgtgat tgaatggctt tctgctcaaa
     8221 atcctaaagc aacttttgat cctaataaac cacaactgcc tggccaatca aagccaggac
     8281 tggggagttt aaattatatg atggaattaa tgtacatagt tggtgagcag gtagtgaagg
     8341 atggtttcat ggatgttcct gatcattttc atggtgcagt aatgtattca agaaaattca
     8401 agttttttaa tccaagtcat gaagcaatat taagagctat cctgcgcgat attaaagggt
     8461 attcgctttc ggatatgtca tggggtatga ttacagggac tatcattgat aaaaatacaa
     8521 acaatccgca ggtttatgat ccatcagaac aaatatttcc tgtatctaaa cgaatgaaag
     8581 aatattttga atcaaagaaa tacaaagtta aatttaaaaa aatttatgaa tctaaaaaat
     8641 actattttga ttatgaaaaa atgtgtcaga tacgagagaa gatgttgcat gaaaacaaaa
     8701 cggatgagtt ataaaaactt atctattcct tcctagtttt tcatgactgg aaacccattc
     8761 tcctcctgaa atcactgctg ttgccagtga aaattcacca cataaaactg ttgcagcaat
     8821 aatttctgca aatttatata ctttacctgc accataacaa cccaggattt caaggcattc
     8881 tttttgagtt ggcaaccctg ttccgccacc gtacgttgca attattaatg aaggtaatgt
     8941 aataaaataa taataatccc cattttcagt tacttctgca taggcaatag cagatgatga
     9001 ttctgcaata tttgcaacat cctgaccagt tgcaataaag atagcagtta tagcatttgc
     9061 agaatgaagt ccattattgt tggctccaga aataaatgct ccaagtgtgg tggtgtatct
     9121 catttttact acagcttctg gtggaagttt cataaaatcg ataaatagtt ttctgggaat
     9181 tgttatttct gctgtaactc tttttcctct agtacttaaa gtattcagat gagaatattt
     9241 tttatcggta tcaatatttc cagaaagagt ataccgttta attgtagggt tgtttttcat
     9301 tatccagtta catgccaccc atgttgcttt gcctaccata ttttgaccgg ctgcatcgcc
     9361 ggttgaaaaa ttgaatcgta aaaacatcat attaccaaca tggaattttt caattttctt
     9421 aagtttacct gtttttgtag tgctttctgc ctgttcttta atatcataaa aatgttcatc
     9481 aatccattct ccaaaatttc gagcaaagat ggcattttca aaaacaaaga caggagctct
     9541 ttgcatagaa tcatcactaa ctgttgtagt aattccacca gcctcataaa ataatttcat
     9601 gcctctgtta taactggcaa ccaatgtacc ctcggtagtt gccaatggaa cataaaattc
     9661 gccattggcg tgttcaccat gtattaaaag tggccctgct attccaatag ggacctgtgc
     9721 tacacctgta aaatgttcaa tgtttcccgg aagaactttt ggatcaaaag aaaatgaact
     9781 aacatgatgt aattctgttc ctgtcattgt tgtgataaaa ttttgacgtc gttttgccat
     9841 ttcgagagta tagtcgttac tgtcatttct tggtatttta gttctttcta acataaactc
     9901 ccctccgtac taaaaatgat taaatgattg gcaatgaagt atattttcca aaaataataa
     9961 gttaagatag gtataaattg tagaaggcaa aaatagaaaa gaaagatatt tcaatgagtg
    10021 ctggttttaa aaaaacatta tgatgccagc actcattata aatgaattag tggcttgaat
    10081 tgttcatata ataggtttaa gtacttctta atcatttaag cttctgcatt tcctcctctc
    10141 taaggacttt tcgtaatatt ttcccaacat tactcttggg taattctgtt ctaaattcaa
    10201 ctattgttgg taatttatat ttggctaatt ttgttgcaca gtaatcaatt atttcttttt
    10261 cggtggcagt ttcaccttct ttgagaacga caaatgcttt aatttgttca cctcttgtag
    10321 gatggggaat ccctatacag caagcttcca gaaccttggg atgagtatat aaaacttcat
    10381 caatttcgcg cggataaaca ttgtagccac cagatattac catatccttt ttcctgtcta
    10441 ctatgaagaa atagccatca ttatccatta tcgcaatatc tccagtatag agccaaccat
    10501 tcctgatagt tttggctgtt tcatctggcc tattccagta tcctttcatt atttggggtc
    10561 ctttaataat catttcacct ggctgaccca cagggacatc attttctcct gtttcaaggt
    10621 caacaattct aacttctgta cttggatatg gaattcctat acttccagat ttttgtacag
    10681 ttccatatgg atttgagtgt gtaacaggtg atgcttcggt taatccatat ccctcattaa
    10741 tatgaacacc agctttttct tcatatgcct ttataacttc taagggaaga ggggcacttc
    10801 cacacatcaa tcgcttaata caagtaagat ctaatttttc aaaatccgga tgattgagca
    10861 ttcctatata catagtgggg acaagacacg caatttgtgg gcgatacttt tggattattt
    10921 caataagaga ttctggtgtt ggtcttcctg tcaggattgt tttaaatcca tcataaatat
    10981 ttaaattcat aacagttgat aatccataaa catggaaaaa tggcaatgca cctaccattg
    11041 tcattgcatc acctctgtga attgttgtat cccaggcgtc aatttgctgt aactgttttg
    11101 taaggttgct atgagttagc attgcacctt ttgatactcc agttgttccc cctgtatatt
    11161 gtaattgtgc aatatcttca gttgttaccg tcacttttgg aggttgtgct ggatatttgt
    11221 ttataatatc tttccactta tagacatctt cagcttgttt aggttcaaca gataatattg
    11281 tattggggtc aacatctgaa gggaggtaat cccgtaaaga gacgcaaaca atctgcttaa
    11341 cctgtgtttt tgatctcaaa tcaataattg tattcccaaa aagatccagg gtaactacaa
    11401 cttttgcccc cgaatcgtta aattgatatt caagctcaga aggagaatac aatgggttgt
    11461 gcataactac tatgcctcct atttttatca cagcatagta tgcagctaca caatgaagga
    11521 tgtttggtaa atgtatagct actcgatcac ccttattaat accaaaatca gcaaggcatg
    11581 tagcaaattt attcactgca tctccaagtt ctctatacgt catttcatat ccctgtgata
    11641 caaacgctac tctatcagca tatttattta tagcttcatc aaaataatca tttaaagtac
    11701 agtccacaaa attaatagtg gatggtactc ctttatcata gaattttaac catggtttgt
    11761 ctttatatga gatagatttc ttactcatac ttccctcctt ttaaaatcaa catttattat
    11821 ctataattga taaacacgat atacaatttt tgaagatctt aggggcactg aattattcaa
    11881 aaaaagtgcc ccataatgat caaaaccatt tgtgatatgg atagtttatt tagaagaagc
    11941 taacttcatc agaagtttca caattccaag aatacctaaa ataaaaattc ctcctccaat
    12001 taataatatt gcagttaaaa caaatggatt ttgtggatta ggtgtctgca tataagttgc
    12061 tgtgaaaagt aaagccatga aagcataatt attaaataat acaggcaata tttcacctaa
    12121 tgcaactata caatatacaa atacaagtac aaatagtaga aaggctacaa actgatctaa
    12181 tccagcgcca acaagtgaag taacaattat ttttgctgca attatcatca atataccgaa
    12241 tgcaccacca actagcatat taggtgcaag ttttttatcc atatgagcca taaagaagaa
    12301 tatcataatt aaaaatgcag gccaggcagg aacatgaaga acatgaaaaa ctatttcacc
    12361 aattgctata aggataataa gcagcaaccc aaagataagt ctgtttttgt ctaacttagc
    12421 cataaaaacc tccataaaat taatgattat agtatttttt tgacactatt gtataacatt
    12481 taaaatgtca ataaaaaaat taatgaatga ccataaatta ttaaatttaa atataataaa
    12541 taaacatata aataattata tatattaata atgttaataa ttataattat tttatgttac
    12601 aaatatatac atataatttt taaaataatg ttaaattatt aaaaaacatc ataattacta
    12661 gattatatat tatcgattaa tgttgaatag ggtagttgct tagaaaaaaa attttgtgta
    12721 aaataaatag taatcattgg tgatgtattg ataaaaaaat atttgaagct atatgaagta
    12781 atgaaattat tttaaacagg attttatgta aataaaggtt tttcgtgaat tacttaatat
    12841 gccagttgtg tttgtaattc gattatttta tttatttcat ggatattttt tacaatagat
    12901 tcttcatggt tggaaagtaa aataatgaga gtttgattca gattatatat atacgtaata
    12961 gctgaattta tattcttcat tgtatcacta attatattgg ctacgggatg atttattttt
    13021 ttaaggaaat tttcaaaatc ggtatattca atgccaattt tggatgttat atcattaatg
    13081 caactggcaa tagaataatt acaaagagag atatcttcaa taatttcatt gtttgatgat
    13141 attatggtaa ctatcgcttc ttcattttca tttatcaaat ttatctgtat tgtttgaaga
    13201 ttgacttcaa acttttttaa caacatttgt ttggttaaca gcaaatctga taaattatga
    13261 agaagtgatt ctatatcatc cctcaattga aaaggaacct ttggatgctg tcgcaaccct
    13321 gtccgaagga gtttctttag tagaaatttt atcccatgca tcccgtaact ctcttaataa
    13381 tttgacaaca ttttttaaga tgtcggcgtt tttctgaata ttagcttcta aaagctgttt
    13441 tttgaagaat atatacaggt taaaaagatt acgtgcaaca tcgccacctt cattcatatt
    13501 taaagctaaa attaattcgt taattatatc ctgagcctta aggatattgg tattggcaat
    13561 atcgtaggtt tttggatgca tatgttctat agcgatattg cagaatttaa tggcaccatc
    13621 ataaagcatt acgattaatt taccctgatt ggcggtattt atttctgtat gtttgtactg
    13681 gtcatacgga tttctactgg gtaaagccat gtaatccccc ctcgattata aacgcattgg
    13741 cattaatagt atcggataaa aatatacaaa tctttattga ttaatccaat atgaaacaca
    13801 aataattaac ctaaaatgtg cttaattctt tgcaaaattt cctcgtattt ttcttttgtt
    13861 ttctgtataa tctcatctgg cagaggagga gggggcgaat ttttatccca tgtggttgtt
    13921 tctaaataat cacgtataaa ttgtttatct aaactaaccg gtgaaatgcc aggtttatat
    13981 aatgctgcat cccagaatct ggagctgtcg ggtgttaaaa cttcatcaat taaaataatc
    14041 tctttttctg aaaacccaaa ttcaaatttt gtatcagcca gtataatgcc ggcttttttc
    14101 aatttatcac gggcaaaatc atataaatct aaagttgcct gagctaactt ttcggcaaca
    14161 tcagttccca gtgccttttt catttcttca atggtaatgt tttcatcatg accggacgca
    14221 gctttagtag ctggagtaaa gattggatat ggcaattcgc ttgctaattg caatcctttg
    14281 ggtaattcaa tgccgcacac tttcccggtt ttctggtaat ctttccaccc gcttccaatg
    14341 atatatcctc gtgcaacaca ttcaaaatct atacgttttg ctttttttac cagtacagcc
    14401 ctgtcctgaa aaaattctgg ataatcacag aatggctttg gaaaattttt ataattggtt
    14461 tcaacaatgt gattattaat aaatttaata tttttaaacc ataaattaga tattgtattc
    14521 aaaataatgc ctttgccggg tattccgttt gggaacaccc aatcaaatgc agatactctg
    14581 tcagttgaaa caataattaa atgttcatca tcaactttgt agacatcgcg tactttacct
    14641 gaaaaaagct ttcgtgtgcc ggggactttt gtggtggtta cagcttccat aaagtttcct
    14701 ttctaatata atatttggcg atagctcata gtagcagcca tcagtttgat aattttttaa
    14761 tcagtatatc attaaccatc tgaggatttg ctttgccttt ggattccttc ataacctgtc
    14821 caatgaggaa ttttaatgct ttatccttac cctttttata atcttccact gatttagggt
    14881 tattcttgat tacttcatca atgatagatt caattgcacc agtatcggtt acctgacgta
    14941 atcccttttc gttaataatt gtatcaggat ttttaccagt ttcaagcatt tcttcaaaaa
    15001 cggttttagc tattttccca ctgatggttg catcatctat tcgcttaaga agtatagcta
    15061 aatctttggg cttaacaata aatgaatcga tagtttccgg gtcatcaatc tttgctaaaa
    15121 cctcactcat tatccagttt gatgcttttt ttggctgtgc cccatctgca acaactgctt
    15181 catagtacga tgcaagttgc cttgtactgg ttaacacctg ggcatcgtat tcgggcaatc
    15241 catattgttg cataaatcgt atgcgttttt ggtgaggcaa ttcaggcaat tcttttttta
    15301 attgttcaat gaattctcta gatatctcaa ttggtggtaa atccggttct ggaaaatacc
    15361 ggtaatcatg agcttcttct tttgatcgca tagaaaatgt aatattgcgg tcagcatccc
    15421 ataaacgtgt ttcctgaact attttttcat tttgcagtaa catcttcttc tggcgttcaa
    15481 tttcatattc aagagctaat ttaaccgaac ggaagctgtt taagtttttt atttcaactt
    15541 tttgaccaaa ggttgtggaa ccttttggtt ttaaagatac attaacatcg catcgcaagc
    15601 ttccttcttc catattacaa tcagacactt caatatattt cagtattgaa cgcagattct
    15661 ggagatactg atatgcttca tcaggagtat taatatccgg ttcggatact atctctatta
    15721 gtggaactcc ggttctgttg aaatcgacat aggaaacttt atagtctgag tcttcactgt
    15781 gtatattctt accggcatct tcttccatgt gcaagcgtgt tattccaatc tttttgattc
    15841 cattgtcagt aactatctca ataaaaccat cataacagat tggcttatca tattgagaaa
    15901 tctgatatgc tttaggaagg tcagggtaaa aatagttttt acgatcaaat ttgctgtatg
    15961 tggcaatctt gcaatttaag gcaagacctg cctgtattgc ttttcgtaaa acttccttat
    16021 ttaatacagg taaaacaccg ggaagtccta aacaaacagg acatacctgg gtgttggcct
    16081 gagaaccaaa ttgagtggag caggaacaaa aaattttgga ttgtgtattc agttgtacat
    16141 gaacttccag cccaataact gcatcaaatt ccatataaaa caccatatct ataataatgt
    16201 attgttaatg ctttgattag tcaaagctat cccatatttg tcaactaaaa acgaatcggt
    16261 gatacactaa tcataaaaat tttttctttt tatagctatt gacttttgca ttaattgaga
    16321 ttaatttaca tacaatttct tattattttt tatgggattt tgttatataa tttgttgaag
    16381 atatcattga atagttgatg ttaaaacttt tttctggagg atactatgaa gataatacat
    16441 ttgttatata taattatttg tataacatta ttttcgtttt tcttttctga acatggtttt
    16501 gctgccaatt tgagcgtggg agtttaaacg tggtatacgt catgggatat tgcagtagaa
    16561 caaaatcagg gcggtggtat aataaaaaca gacaggggtt taatgtatgg accggtgcta
    16621 tcggtaaatg taatggataa ttggagcata tctggtgtat ttttatatgg tgaattcaag
    16681 aatcctgagt attccagagg aactgctgat tttggcaatt ttaaacgata tgattttgat
    16741 ggaacaatta attacgggct taccaatttt ctgaaaatat ttattggtgg taaatatgtt
    16801 aagtttgatt ttttagtatc caatcaggat gcatcatatt atatgtatgg gcctggcgca
    16861 ggagcatcag taatttttaa tattgtggat aatctttatt ttacagctaa taccagttat
    16921 gtatatatga aaggtaaggc tgagcttgta gatagttctt catcaatgat tacgaaaggt
    16981 ataaattctt cggcatcttt aggatactat tttgcatcaa catcagttac ggtatatata
    17041 ggaggtagat atcagcgttt atttgaggac agaaaagttg aagattcttt taaataccat
    17101 gaaatgatgg gagttactgc agcagctacc tatagcttta tgttgtaaca gcgtgtcttg
    17161 attaagcgta gacaaaatat ttttatgtgt ttggtatgat aattttattg attttattat
    17221 ctgattgcaa tatactacta aaatagtagt atgttgcaat aaaagaaatg acaatgaaaa
    17281 agatacgtat actgtcaaca aataccgtta tgggtattat actttttact tatatacttg
    17341 cccttatccc ttcccgggca ttgccgcaga caatagattt tatttcagca caaagcaagc
    17401 ttcttgaaaa taaccccaca ataaaagcaa tgcagcttag ggtagaacaa tataaaaaaa
    17461 agattacggt aacatcatcg ttacccgatc caacaatcaa attgggtctg gtcaatattc
    17521 ctgttacaaa actgtcattg aatcaatcag atatgactgg aaaagaaatt gggttctatc
    17581 agatgtttcc ttcatttgga acattatcta caaaagaaag gatagctctt cttgaataca
    17641 aaaaggctat tgaaaacgaa aggttctata cagcatatta cttacataca ttgcgtactt
    17701 atttttttga aatagcgtat ttgcatgagt atctaaaaat tattgaggaa accaaacaat
    17761 accttacggt actcttagat gtacagaaag caaaaagcag tacaggagca ggaagtttat
    17821 cggatatcct gaaaataagt gttgagatgg caaaacttga tgaagaaata att
//
LOCUS       DAORYL010000063        17713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_2619_length_17713_cov_15.713105, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000063 DAORYL010000000
VERSION     DAORYL010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 17713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17713
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2619_length_17713_cov_15.713105"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="PLV81_11110"
     CDS             <1..1089
                     /locus_tag="PLV81_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOM10686.1"
                     /translation="GIVELAEDGEDAPGVVVQGNDRRLKEATEETPGIVTLAKHGETR
                     KNHVVQSDDPRLSDKREPLPHTHDYAPLVHSFESHTGTIAITQEKSGEFVDITPPTHD
                     SAVLYGKNIANTGSAIGVAGITQPSADSEVKSYGVLGHAPMCGVRGQSQGNTGKTKGC
                     GVLGISRFGAGGVFASEHNWSLVADGFGTIAPYDDTLHLTGEGKALLVNGESCFKGKI
                     LLDTHTKDYYANIVEMFETDEQEFISPGDVLVVSTQGNAVLSRSRTAYNKGVIGVVAG
                     NPVVVLNNAAAQTKLYPVVLTGSVLCKVDARQKPVKPGDLLVTSDTPGCGMAASIDSF
                     DKVGTVFAKALTALDDGIALIPVMIWKM"
     gene            complement(1102..1683)
                     /locus_tag="PLV81_11115"
     CDS             complement(1102..1683)
                     /locus_tag="PLV81_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10687.1"
                     /translation="MKKIVWIICIASFALYTTFTPAQQKAPQNTQPQSKSIADGYGNV
                     KWGSTYNDVKPNVLGRIAFTDEKRIIVSKDGELEYRYGFFYPEINQEANQQPKLFYVV
                     IQFPYISLDDMKKKMEEKYGPPTGESIKNNQGAYIWESESTSIIVWVDSYEKKPFCRK
                     ITYLSKVMAKDINAYHNLVFSKKEQEILKQLQP"
     gene            1948..2871
                     /locus_tag="PLV81_11120"
     CDS             1948..2871
                     /locus_tag="PLV81_11120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOM10688.1"
                     /translation="MEDHHEHSHTHTHQISSTGRFVLVVTLNVIITVAEYIGGIVSGS
                     LALISDAGHNLSDVASLILGYIGQKVSQQKPGKKYSFGLKRFEVLIALINAMTLLGIG
                     AYIIYEAIERYINVQPVNPVIMLPVAFIGLAGNVISMLLLYKSRDENLNLKAAFLHLL
                     YDAVSSVGVIIVGFVLLVNSSWVVLDLVVSVAIAFMIVASSLDIIKSSFRIFLQGVPT
                     RIDFDEVYNSILTVPHVDTVHGLHIWSVDSNEVFLSCHVCISGGDSSLNTDSIIKAIN
                     GMLEEKFGITHTTLQIEHTNLCTLEGGSCCR"
     gene            2874..3200
                     /locus_tag="PLV81_11125"
     CDS             2874..3200
                     /locus_tag="PLV81_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10689.1"
                     /translation="MSIYFTHPQLGQEVESIAGVYAYTEEKRLQIDEKDVLCFIGYSV
                     TNKSCCGVGGCMFATVAGFVQQYAVKKGEDNIVSMVEPITDTALRKQIEKLIKAQGVL
                     QVNFAD"
     gene            complement(3197..3619)
                     /locus_tag="PLV81_11130"
     CDS             complement(3197..3619)
                     /locus_tag="PLV81_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOM10690.1"
                     /translation="MDYKHLTAPCGLDCFNCPMYLAGSDDNLRKKIAQSLNISFEKAV
                     CKGCRNEQGKIEFLHMQKTCSVYACTQSKGIDFCYECNDFPCDNLHPYADRASQVPHN
                     TKVFNLCLIKKMGLEKWAAEKAHSVKKTYFSGTFKLTD"
     gene            4025..5698
                     /locus_tag="PLV81_11135"
     CDS             4025..5698
                     /locus_tag="PLV81_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009793785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOM10691.1"
                     /translation="MGAFKELTMWQALHGTVEKYPDNEALVYPEFGIRMTYAQFYQKC
                     REVAKGLIALGIQKGDHVSLWATNVPEWVYLQFSLGMIGGVLVTVNTNYKSHELEYLL
                     KQSDSTTLFMIERYRDSNYYQIIREIIPNLDETQPGNINATNLPLLKNIVFIGKRDSL
                     PGMFTFDDIVLMGSSLSDAELDKRINECDIYDVINMQYTSGTTGFPKGVMLTHYNIVN
                     NAIMVGDVMGMTSSDRLLIHVPLFHCFGCVMSTLNCVTHGSTMVVLEYFDPLKSLQAV
                     QNEKCTAINGVPTMFIAMLNHPDFRKYDMSSLRTGIMAGAPCPVETMNQVRTIMHCPE
                     VVIAFGQTECSPVMTMTRRDDPVDLRVSTVGRLLPGIEGKIVDPETGEDLPPYTQGEI
                     VTRSACVMKGYYNMPEATTNAIDKEGWLHTGDLGSVDEHGYFKVTGRIKDMIIRGGEN
                     IYPREIEEFLLTNPKIKDVQVVGIPDEKYGEQVLAVIQLKDGQSATAEEMVQFCTGRI
                     ARHKIPKYWEFVDTFPMTASGKIQKYKLRDMFAQKYGKAASVFEAIQKQ"
     gene            5710..6099
                     /locus_tag="PLV81_11140"
     CDS             5710..6099
                     /locus_tag="PLV81_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOM10692.1"
                     /translation="MIEEKITTYSIGEIAKLLEMSTRTIRYYEELGLLNSVKRIENGR
                     RIYTDDDVRRLKLIKRLKILGLTLSEMHELESIWQIHKTNDIVLRRLLEILDSHVHRV
                     DDRIRDLEILKNEIIEYQTRIKRKLNE"
     gene            complement(6102..6830)
                     /locus_tag="PLV81_11145"
     CDS             complement(6102..6830)
                     /locus_tag="PLV81_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOM10693.1"
                     /translation="MKEKIIIHNLHKSFGQQIIFSGLNLTVYEGEILCIIGKSGSGKS
                     VLLKHCIGIIKPDSGTISVDGQPLTYTDDSAILKKFGVLFQGGALFDSMNVYDNIAFP
                     LRRQGISEEVIAHKVPELLAHVGLKGIESKSPSELSGGIQKRVALARAIAKSPEIMLY
                     DEPTTGVDPITAGSVDRLIVYMRNTFGMTSVVVTHDMRSAFRIADRIAMLHNGTILFE
                     GTPQQLQECQDAVVRQFIAGKASA"
     gene            6968..7207
                     /locus_tag="PLV81_11150"
     CDS             6968..7207
                     /locus_tag="PLV81_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOM10694.1"
                     /translation="MKVFQVGELKSKFSNVLDYIRKGEEITISYGKKKEKIAVIIPYS
                     KYKKAKPRKLGILKNKASFKIHDDFMISDEELLHL"
     gene            7204..7602
                     /locus_tag="PLV81_11155"
     CDS             7204..7602
                     /locus_tag="PLV81_11155"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOM10695.1"
                     /translation="MNYLLDTHVFLWTLFNDELLSKNAVSIISNPENTVLVSVISFWE
                     ISLKYNAGKLSLEGVLPDELPACAEKAGLEIMNVTPAEVSSFYKLPILKHKDPFDRLL
                     VWQCINNNICLISKDSDLAEYKNYGLQIMW"
     gene            7900..9177
                     /locus_tag="PLV81_11160"
     CDS             7900..9177
                     /locus_tag="PLV81_11160"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOM10696.1"
                     /translation="MKIRCGLIGFGTVGSGVYTLLQKHRTDIYKKTGVDIELVTICDV
                     RDIKDKVNVPVTGSWQDVVNDASIDVVIELIGGIEPAKTIILSALNAGKSVVTANKKL
                     LAESGNDIFTASQKAKGALAFEAAIGGGIPCVAALRNGLVGNAIQSVVGILNGTTNYI
                     LTRMAEDGMSFDEALKLAQAKGYAEADPTFDIEGFDAAHKIALLSMLAYNVRVSMQNI
                     YIEGITKITSLDIKYAHEMGYTIKLLGVSRMVDDKLDIRLQPVMIPFTHPLASVRNEF
                     NAVMYNGDMTGAVTLYGKGAGSLPTASAVVSDIVSIAQKQNGIKDSVYIANDAQYLSS
                     DDRMLRYYVRLHTEDRAGILSKISGVFASYNISIASVIQKEVNAPYVPLVITTHAARE
                     AAMHKAKQDIEQFNFVNGQVMVIPIEDFNSTGE"
     gene            9180..10535
                     /gene="thrC"
                     /locus_tag="PLV81_11165"
     CDS             9180..10535
                     /gene="thrC"
                     /locus_tag="PLV81_11165"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008672001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOM10697.1"
                     /translation="MSDSNYKAVFRCIAGCNEEYPLDEVVYRCKKCGELLEVHHDIDR
                     LKELSPKQWRELFDSRVGTTKWPYGSGVWSKKEWVCPEVNNENIVSMFEGNTNLFWAK
                     RFGAMIGMDDLWVKMCGNSHTGSFKDLGMTVLVSMVNEMIKRGKPIKAVACASTGDTS
                     AALAAYCAYAGIPSIVFLPRNKISTAQLVQPMSNGSIVMSLDTNFDGCMKIVQEITKD
                     NSIYLANSMNSLRIEGQKSISIEIVQQFDWEVPDVIIIPGGNLGNVSALGNGFEMMLQ
                     LGLISKKPRIVLAQAQNANPLYLSYLNGFKEFKPITPHKTLASAIQIGDPVSVKKAIR
                     ALQKFDGIVEQASEEELANAAALADRTGMYSCPHTGVALAVLLKLVEKQVISKKDRTV
                     VISTAHGLKFSEFKVGYHEGTLENVTCKYANRPIELEANVDVVRQVLFKELDKRSRLE
                     Y"
     gene            10557..11216
                     /locus_tag="PLV81_11170"
     CDS             10557..11216
                     /locus_tag="PLV81_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOM10698.1"
                     /translation="MKITQHVYQVGGSNLTHPSDAAIYCITDGKVSALIDAGTGDGHD
                     RLIKNLDREHIAASSIQYLFLTHCHFDHVGGAHKVRGTFNCKVVAHELDAHYIETGDQ
                     YVTAASWYGSFLTPCTVDIKVNEKQKTFEVGSLNVTMYHIPGHSPGSTALTVESEGKL
                     VLFGQDVHGPLNDVLKSNRDDYIQSLEFLLSLNADILCEGHFGVYYEKEEVKDFIESF
                     L"
     gene            complement(11273..13144)
                     /locus_tag="PLV81_11175"
     CDS             complement(11273..13144)
                     /locus_tag="PLV81_11175"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOM10699.1"
                     /translation="MMRNPLVDQRDVQFVLFELLKIDGLKQYQKFADQDKDMYEATID
                     LALKMAVEEVYSVNKEADKTGVKYDPETKKVMIPEGYKNALRKYNEAGFVGMIEDPEI
                     GGMGMPGAVAMACSEIFTSASLSFTTYPGLSHGAAALIKNFGTEEQKQMYLEKMLTGQ
                     WGGTMCLTEPEAGSDVGNLKTKAIPQPDGTYLIQGQKIFITSGDNDFYENIIHPVLAR
                     IEGDPPGTKGISIFIVPKYRVKPDGSLGEFNDVICSGIEHKMGIHGSATCTLNFGDNG
                     KCVGWLLGQPRQGMKIMFHMMNEARLGVACQGLALSTTAYMHAVTYARNRIQGVHVTQ
                     MLNPEAPKVAIIEHPDVKRMLLWMKAYVEGMRMLNYYLAHNATLIEVLDGDAKKEAQG
                     MVEILIPIAKAGCTDTAVLVTSEAIQVYGGYGYTSDYPVEQYMRDAKITQIYEGTNGI
                     QSMDLTMRKILMNPEQYNYNIMKKRMAETIAKAKGIVEDKYIELVEKGIQKLDEVITM
                     LKDQMAAGRFLHIFAACTPLQQAMFMLTLAWLHLWSLTLTIPKMKELVGDKKGEERDK
                     FLADNEEAAYYSGRVLSSQFYLGAEFPKFFGRIDALLFNETAVIKASKDIFTGALLE"
     gene            complement(13398..15281)
                     /locus_tag="PLV81_11180"
     CDS             complement(13398..15281)
                     /locus_tag="PLV81_11180"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOM10700.1"
                     /translation="MISVSMRNQLADQRDVHFALFELLKLDELKKIPRFADHDKDIYE
                     ATIDLAFRMAVEEVYPVNREADKIGVKYNPEAKKVIIPDLLKAPLKKYNEAGFVGIME
                     EAEIGGMGMPFTIAIACNEIFTAASLAFTSYPSLAHGAAALIKNFGSEEQKQMYLEKM
                     LTGQWGGTMCLTEPEAGSDVGNLKTKAIPQPDGTYLIQGQKIFITSGDNDFYENIIHP
                     VLARIEGDPPGTKGISIFIVPKYRVKPDGSLGEFNDVICSGIEHKMGIHGSATCTLNF
                     GDNGKCVGWLLGQPRQGMKIMFHMMNEARLGVACQGLALSTTAYMHAVTYARNRIQGV
                     HVTQMLNPEAPKVAIIEHPDVKRMLLWMKAYVEGMRMLNYYLAHNATLIEVLDGDAKK
                     EAQGMVEILIPIAKAGCTDTAVLVTSEAIQVYGGYGYTSDYPVEQYMRDAKITQIYEG
                     TNGIQSMDLTMRKILMNPEQYNYNIMKKRMAETIAKAKGIVEDKYIELVEKGIQKLDE
                     VITMLKDQMAAGRFLHIFAACTPLQQAMFMLTLAWLHLWSLTLTIPKMKELVGDKKGE
                     ERDKFLADNEEAAYYSGRVLSSQFYLGAEFPKFFGRIDALLFNETAVIKASKDIFTGA
                     LLE"
     gene            complement(15938..17329)
                     /locus_tag="PLV81_11185"
     CDS             complement(15938..17329)
                     /locus_tag="PLV81_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HOM10701.1"
                     /translation="MKLLLPFITISILLFVGHYLRLHIKLFQKLYLPSSLLGGIVGLM
                     VYQTAKTLSINTLTTWFDGLAMLPSFLINIVFACLFIGVSLPSFKIVLKKSIPQLSYG
                     QIVAWGQYMVGLGLFIFIISKVWNLPAMFGAVIEVGFEGGHGTASGLAPTFEMMKWPQ
                     GKDFALASATVGIISAVIAGIALINWANLKGYTHRSLQVENIPEDETIGIIPVDRRPI
                     AGYLSIHTDSLDAMSYHVAIVGIAVGIGYFLKEGLVAGIYCLQTVLSHATYTNIISII
                     KSFPLFPLCMIGGLIVQLFAQHFDRHETIDLGLVRRIQNMALDILIVSAVAMIQVVIV
                     IQGIVPFTIMVIGGILWNIFCLLVLAKRLLPDSWFERAIAEMGQSMGVTATGLMLLRI
                     VDPDYRTPALEAFAYKQLLHEPFMGGGIITSMFIPLLGIGGITMAYTLFYISITVIIL
                     WLVILLLFKYKLL"
     gene            complement(17523..>17713)
                     /locus_tag="PLV81_11190"
     CDS             complement(17523..>17713)
                     /locus_tag="PLV81_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10702.1"
                     /translation="TVVDTRFKIAYGFSGKCTVNAQSNHSMAQNLLDLGLDGKKPLGC
                     MYSPQAQMMLCKGEPIVS"
BASE COUNT         5262 a         3342 c         3776 g         5333 t
ORIGIN      
        1 ggaatcgtag agttagcaga ggatggagaa gatgcaccgg gtgtagtggt acagggcaat
       61 gatagaaggc ttaaagaagc aaccgaggaa actcctggaa ttgtaacact ggcaaaacat
      121 ggcgaaaccc gtaaaaacca tgtggttcag tcggatgacc cacggttaag tgataaacgt
      181 gaaccattac cacacacaca tgactatgca ccgctggtgc actcatttga aagtcatacc
      241 ggaactatag caattactca ggaaaaatca ggtgaatttg ttgatattac accacctact
      301 cacgacagtg cagttttgta tggtaaaaat attgcaaaca caggcagtgc tattggtgtt
      361 gctggtatta ctcagccttc tgctgattca gaggtaaagt catacggtgt gttaggacat
      421 gcaccaatgt gtggtgttcg cgggcaatcc cagggaaata ccggtaagac taaagggtgt
      481 ggagtgcttg gcatatcgcg gtttggagca ggtggtgtgt ttgcaagtga gcataactgg
      541 tcgcttgtgg ctgatggctt tggaactatc gccccctatg atgacacgct gcatttaacc
      601 ggtgagggca aggcactttt agttaacggt gagtcctgtt ttaaaggcaa aattctgctg
      661 gatactcaca caaaagatta ctacgccaat attgttgaga tgtttgaaac cgatgaacag
      721 gaatttattt cacctggtga tgttcttgtg gtaagcactc agggcaacgc tgttctttca
      781 cgttcacgca ctgcatataa taaaggggtt attggcgttg ttgcaggcaa tccggttgtt
      841 gttttaaata atgcagcagc acagacaaag ctgtatccgg tagtattaac aggctcagtg
      901 ttgtgcaagg tggatgcacg ccaaaagcca gttaaacctg gtgacctttt agtaacatca
      961 gatacaccag gctgcggtat ggctgcttca attgattctt ttgataaagt gggcacagtg
     1021 tttgcaaaag cactcactgc gctggatgat ggtatagctt tgattccggt aatgatctgg
     1081 aagatgtagc tactaccgta tttatggttg aagttgtttg agtatttcct gctctttttt
     1141 gctgaaaaca aggttgtgat aggcattgat atcttttgcc attaccttgc ttaagtacgt
     1201 tatcttgcgg cagaagggct ttttctcata actatcgacc cacacaataa tagatgttga
     1261 ttctgattcc cagatatagg cgccctgatt attcttaatt gattcaccag ttggcggacc
     1321 gtacttttct tccatcttct ttttcatatc atccaatgaa atatatggaa actgaataac
     1381 cacataaaac aattttggct gctggtttgc ttcctgattt atttcaggat aaaaaaagcc
     1441 gtagcggtat tctaattctc cgtcttttga cacaataatt cgcttttcat cggtaaatgc
     1501 tattcttcct aacacattgg gttttacatc attgtaggta ctaccccatt ttacattccc
     1561 gtaaccatcg gcaatagatt tactctgcgg ctgagtgttc tggggcgctt tctgctgtgc
     1621 aggtgtaaag gtggtatata atgcaaaact tgcaatgcag attatccaaa ctattttttt
     1681 catagtacta tccttcatac cagtttatgt actgtttata ctatcggtta atcttatgtt
     1741 aaaatatatt ataatttatg ggcgatagtt ccggtaatat gcttttgtat atatacattc
     1801 tgtaaagatg actattttgt attacagtag tgaataaaaa tccaatccag gcggagtaat
     1861 attataaaaa taattcttgt aaaaattttt gcacagtaac tattgtatac tatactatag
     1921 taattacttc tatattaggg aaaagacatg gaagaccatc acgaacattc tcatacacac
     1981 acacatcaaa tcagttccac cggacgattt gttctggttg tgacactgaa tgtcatcata
     2041 acagttgcag aatacattgg tggcatagtg tcaggaagcc ttgcgctcat ctcggatgcg
     2101 gggcataatc tttccgatgt tgcatcactt attttaggat acattggtca aaaagtgtca
     2161 caacaaaaac ccggaaagaa atattccttt gggctcaaac gttttgaggt actcatagcg
     2221 cttatcaatg ccatgacact tcttggtatt ggtgcttaca ttatttatga agcaattgaa
     2281 cgctatatta atgtacagcc ggtaaatcca gttattatgc ttcccgttgc gtttattggc
     2341 cttgcaggta atgttatctc aatgctgctt ttatataaaa gcagggatga aaatcttaat
     2401 ttgaaggctg catttttaca tcttttgtat gatgcagtat cctcggttgg cgttattatt
     2461 gtgggttttg tactgcttgt gaacagtagt tgggttgtgc ttgatttagt ggtaagtgtg
     2521 gcaattgctt ttatgatagt ggcaagctct cttgatatta tcaaaagctc ttttagaata
     2581 tttctgcagg gtgtgccaac gcgtattgat tttgatgaag tgtataattc catactgact
     2641 gtacctcatg ttgatacagt gcatggactg catatctggt cagttgactc aaatgaagta
     2701 tttttatcat gccatgtgtg tatcagtggt ggcgatagtt ccctgaatac ggattccatt
     2761 attaaggcaa ttaatggcat gcttgaagaa aaatttggca ttacccatac caccttacag
     2821 atagaacata ccaatctgtg cactctggaa ggcggaagct gttgcaggta accatgagta
     2881 tatactttac ccatccgcaa ttaggtcaag aagtagaatc aatagcaggt gtgtatgcat
     2941 atactgaaga aaaacgcttg cagatagatg aaaaggatgt gctgtgtttt attggatatt
     3001 ctgtgaccaa caaaagctgc tgtggtgttg gaggatgcat gtttgctact gttgcaggat
     3061 ttgtgcaaca gtatgctgtg aaaaaaggtg aagacaatat tgtttctatg gttgaaccaa
     3121 taacggatac agcactacgg aaacaaattg aaaagctcat aaaagcgcag ggggtgctac
     3181 aggtaaattt tgcagattaa tctgtaagtt taaatgttcc gctgaaatat gtcttcttaa
     3241 cgctatgtgc tttttcagct gcccactttt caagtcccat tttcttaatc aggcataaat
     3301 taaaaacttt tgtattatgg ggaacctgtg acgctctatc agcatatgga tgaaggttat
     3361 cacaggggaa atcattgcat tcatagcaaa aatcaatccc ttttgactga gtgcaggcat
     3421 atacgctgca ggttttttgc atatgtagaa attcaatctt accctgttca ttgcggcacc
     3481 ctttacatac cgctttttca aagctaatgt tcagtgattg tgcaattttt tttcgcaggt
     3541 tgtcatcgct tcctgccagg tacatagggc agttaaaaca atcaagacca catggtgcgg
     3601 taaggtgttt gtaatccata agagaatccc ccgaatgaga attgtaaagt aaagtgctat
     3661 ttattacagt tactatcgca tagtataata ccataggagt ttgtaaagat aaatattaat
     3721 tttcatgtta ctcaataaat ggttggacat ccaattaatt attgacagaa aagaaggtat
     3781 agtgtagtgg tacaaaaaat gatgatgctg gataggcaac ggtggagtac ttagtagcat
     3841 tattattttg aaagcataaa aaatttatca aaggagttac atttcagcaa ataataaatt
     3901 tttatgataa aatgttgaca tataatggca tgattttaca gtttacgtta actggattca
     3961 ttataagcat tatagtatat ttatattaac atataattct ttatataaga aaggagaggt
     4021 tggtatggga gcttttaaag aattaaccat gtggcaggcg cttcatggta cagttgaaaa
     4081 atatcctgat aatgaagcac tggtataccc tgagtttggc atacgcatga cgtatgctca
     4141 gttttatcaa aaatgcaggg aagttgcaaa agggttaatt gcactgggca tacagaaggg
     4201 tgaccatgtt tctctgtggg caaccaatgt gcccgaatgg gtatatcttc agttttcact
     4261 gggtatgatt ggcggagtgc ttgttacggt caataccaat tataaatccc atgaactgga
     4321 gtatctcttg aaacaatcag attccaccac attgtttatg attgaaagat accgtgatag
     4381 caattattat cagataatac gtgaaattat acccaatttg gatgaaacac aaccgggtaa
     4441 tattaatgca acaaatctcc cattgctgaa aaatattgtt ttcattggga agcgggattc
     4501 attaccaggc atgtttacgt ttgatgatat agttcttatg ggaagttctt taagtgatgc
     4561 tgaactggat aagcgtatta acgaatgtga tatttatgat gttatcaata tgcagtacac
     4621 atccggtaca acaggatttc ccaaaggtgt tatgcttact cattacaata ttgtcaacaa
     4681 tgctatcatg gtgggcgatg tcatgggtat gacttccagt gataggctgc ttattcatgt
     4741 cccattgttc cactgctttg gctgtgttat gagtacatta aactgtgtaa cccatggttc
     4801 aacaatggtg gtactggaat actttgatcc gttgaaatcg ttgcaggcag tacagaatga
     4861 aaagtgtaca gcaattaatg gggtccccac aatgtttatt gcaatgctca atcatcctga
     4921 ttttcgcaaa tatgatatgt catcactcag aaccggcatt atggcaggag ccccgtgccc
     4981 ggttgaaaca atgaatcagg tgcgaactat tatgcattgt cctgaagttg ttattgcttt
     5041 tggccaaact gagtgctcac cagttatgac catgacgcgc cgtgatgatc cagttgattt
     5101 gcgggtttca actgttggtc gcctccttcc aggtattgaa ggtaagatag ttgatcctga
     5161 aacaggtgaa gatttaccac cctatacaca gggtgagata gttacccgaa gtgcatgtgt
     5221 tatgaaaggc tattataaca tgcccgaggc cactaccaat gccattgaca aagaaggctg
     5281 gctacacact ggcgatctgg gttcggtgga tgaacatggc tactttaagg ttaccgggcg
     5341 tatcaaagac atgattatcc gtggtggcga aaatatctat ccacgcgaaa ttgaagaatt
     5401 tttgttgact aatccaaaga taaaagatgt acaggtagta ggtattcctg atgaaaaata
     5461 cggcgaacag gtacttgcag taatacagct taaagatggg caaagtgcaa ctgctgaaga
     5521 aatggtgcag ttttgcacag gaagaattgc ccgccataaa atccctaaat actgggaatt
     5581 tgtggataca ttccccatga ctgcaagtgg aaagatccag aaatacaagc tcagagatat
     5641 gtttgcccag aaatatggaa aagcagcttc agtatttgaa gcaattcaaa aacagtaata
     5701 agggtacagg tgatagaaga aaagataaca acatattcaa taggcgaaat tgcaaagctt
     5761 cttgagatga gtacgcgcac catccgctat tacgaggaat tgggtttgct caactcagtg
     5821 aagcgtattg aaaacggaag gcgtatttat accgatgatg atgtcaggcg cttgaaactc
     5881 atcaagcgcc tgaagattct gggtttaacg ctttcggaaa tgcatgaact ggaatcaata
     5941 tggcaaatcc acaaaaccaa tgatatcgtg ctaagacgcc ttcttgaaat tcttgattcg
     6001 catgtacata gagttgatga caggatcagg gatttggaga tattaaaaaa cgagatcatt
     6061 gagtaccaga caagaatcaa acgcaaactg aatgaataaa ttcacgccga agcttttcct
     6121 gctatgaatt gtcgcaccac tgcatcctgg cattcttgca gttgttgtgg tgtgccttca
     6181 aaaagtattg tgccattatg aagcatggca atacggtcag caatcctgaa tgctgaacgc
     6241 atatcgtgtg ttaccaccac cgatgtcatg ccaaaggtgt tgcgcatata cacaatgagg
     6301 cgatcaacgg atcctgcagt gatggggtca acgccggtag tgggctcgtc atacagcatg
     6361 atttcagggc ttttggcgat agctctggca agtgcaaccc gcttttgaat accacctgaa
     6421 agctctgaag ggcttttaga ctcaatgcct tttaaaccaa catgtgcaag taactcagga
     6481 actttgtgag caataacctc ttcactgata ccctgtcgtc tgagtgggaa agcgatgtta
     6541 tcgtacacat tcattgaatc aaaaagcgca ccaccctgaa agagaacacc aaattttttt
     6601 agaatggcag aatcatcagt ataggtgagt ggttgtccat ctacactaat ggtgccgcta
     6661 tcaggtttaa ttataccaat acaatgcttc agcaataccg atttcccact gccactttta
     6721 ccaatgatgc ataaaatctc accttcatac acggttaaat tgaggccgga gaatataatc
     6781 tgctgcccaa atgatttgtg caggttgtgt atgattattt tttctttcat aatatataat
     6841 tatatatatt cacacatatg aaacagttta atctaaaaat actggtggtc aaaagtcaag
     6901 caattgattt tgaaatattg cataattaaa gttgacaact tgacaacttt atagtatctt
     6961 gtctgttatg aaggtatttc aggttggtga attaaagtca aaattttcta atgtacttga
     7021 ttacattaga aaaggcgagg agattactat ctcctatggg aaaaagaaag agaaaattgc
     7081 tgttataatt ccgtattcaa aatataaaaa agctaaacca agaaagttgg gtattttaaa
     7141 aaataaagct tcttttaaaa ttcatgatga ttttatgatt tctgatgaag aattgttaca
     7201 tttatgaatt acctgcttga tacccatgtt tttttgtgga cattgtttaa cgatgaatta
     7261 ttatcaaaaa atgctgtttc aattatttca aatcctgaaa atactgtttt agtaagtgta
     7321 atcagttttt gggaaatttc gttaaaatac aatgcaggca aattatcact agaaggagtt
     7381 ttacctgatg agttgcctgc atgtgccgaa aaggctggtt tggaaataat gaatgtaaca
     7441 cccgctgagg tttcttcttt ttacaaacta ccaatactaa aacataaaga tccatttgat
     7501 aggttactgg tgtggcagtg tatcaataat aatatctgtt tgatttccaa agattcagat
     7561 ttagctgaat acaaaaacta tggattacaa atcatgtggt aatagggata agttattacc
     7621 attgtgcgat ggaattcctg tttagatgac tttaaaactt aaaaaaatac atatataatt
     7681 acagttgaca acgaaacctg tatcatcgta tgaatgtgat gtacatactg taatgaaaaa
     7741 cttttatctt tgtgcatatg aaaaccgttg ttggccaatt cgacaaaagg ttgtattgcg
     7801 tattttttaa ttcattgacc atatattaaa gaaagtgtgt ataggtaaaa tcagaatgat
     7861 tgagggcaag atggatattt tttatattcg aggtatgcta tgaaaatacg atgtgggctt
     7921 attggttttg gtactgtggg aagcggtgtg tacacgctgt tgcaaaagca cagaacagat
     7981 atatataaaa aaacgggtgt ggatattgag cttgtcacaa tatgtgatgt acgtgatata
     8041 aaagataagg ttaatgtgcc ggttacaggt agctggcagg atgttgttaa tgacgcttct
     8101 attgatgttg tcattgagct tattggtggg atagaacctg caaagacaat tattctttct
     8161 gcacttaatg cgggaaaaag tgtggtaaca gcaaataaaa agcttttagc tgaaagcggg
     8221 aatgatattt ttaccgcttc acagaaagca aaaggggcac tggcgtttga agcagccatt
     8281 ggtggtggaa ttccgtgtgt tgctgcgttg cgaaacgggc ttgttggcaa tgccatacaa
     8341 tcggtagttg gtatccttaa cggtaccacc aactacattt taacacggat ggctgaggat
     8401 ggcatgtcgt ttgatgaagc attgaaattg gcacaggcaa aaggctatgc tgaggctgac
     8461 ccaacctttg atattgaagg ctttgatgca gcacacaaga ttgcgctatt gtcaatgctg
     8521 gcatataatg ttcgtgtttc aatgcagaat atttatatag aaggcattac aaaaattaca
     8581 agtttggata taaagtatgc ccatgaaatg ggctatacca tcaagctttt aggggtcagc
     8641 cgcatggtag atgataagct tgatatccgc ctgcagccgg tgatgattcc gtttacccat
     8701 ccgctggctt cagtgcgcaa tgaatttaac gcggttatgt ataatggcga tatgactggc
     8761 gcagtgaccc tgtatggcaa gggagcaggc agtttgccca cagcatcagc cgtggttagt
     8821 gatattgttt ccattgcaca aaaacagaat ggcataaaag acagtgtgta tatagcaaac
     8881 gatgcgcagt acctttcatc ggatgacaga atgctgcgct attatgtacg gttgcatact
     8941 gaagataggg caggtatttt gtcaaagatt tctggcgtgt ttgcttcata taacatcagt
     9001 attgcttctg tcatacagaa agaggttaat gcgccgtatg ttccgcttgt cattaccacg
     9061 catgcagcac gtgaagctgc aatgcataaa gccaaacagg atattgaaca gtttaatttt
     9121 gtcaatggtc aggttatggt cattcctatt gaagatttta atagtacagg tgaataatta
     9181 tgagcgatag taactataaa gcagtttttc gatgtattgc aggttgtaat gaagaatatc
     9241 cccttgatga ggttgtctac aggtgtaaaa agtgtggtga actgttagaa gtgcatcacg
     9301 acatagaccg attaaaggaa ctatcgccca aacaatggcg agagcttttt gacagccgtg
     9361 taggaaccac aaagtggccg tatggaagcg gagtgtggag caagaaggaa tgggtgtgcc
     9421 ctgaagttaa taatgaaaat attgtgtcaa tgtttgaagg caataccaat ttattctggg
     9481 caaaacgttt tggagcaatg attgggatgg acgacctgtg ggtaaagatg tgcggtaaca
     9541 gtcatactgg ttcattcaag gatttaggga tgacagtact ggtgtccatg gtgaatgaaa
     9601 tgataaagcg gggtaagccc attaaagcgg ttgcctgtgc atcaacgggt gatacatcag
     9661 cggcgctggc agcctactgc gcgtatgcgg gtatcccatc tattgttttt ttgccgcgca
     9721 acaaaatttc tactgcacag ttagtgcagc ccatgagcaa tggttccatt gtcatgtcac
     9781 ttgatactaa cttcgatggc tgtatgaaga tagtacagga aataacaaag gataacagta
     9841 tctatctggc aaattcaatg aactcactca ggattgaagg ccagaagtca atctcaattg
     9901 aaattgtgca gcagtttgac tgggaagtgc ctgatgtcat cattattcct ggtgggaatt
     9961 taggcaatgt gtcagcgctt ggcaacggat ttgagatgat gctgcaactg gggcttatca
    10021 gtaaaaaacc acgtattgtg ctggctcagg cgcaaaatgc caatccgctg tatctatcgt
    10081 accttaatgg ctttaaggaa tttaaaccaa taacaccgca caagacgtta gcctcggcaa
    10141 tacagattgg cgatcctgtt tcagtcaaaa aggcaatacg tgcattgcaa aagtttgatg
    10201 gcattgtgga gcaggcaagt gaagaagaac tggcaaatgc tgcagcgctg gccgaccgta
    10261 ctggcatgta cagctgccca catactggtg tagcactggc ggttttgctg aagcttgttg
    10321 aaaagcaggt tatatctaaa aaagacagga ctgtcgttat atcaacagcc catggattga
    10381 agttcagtga gtttaaagtt ggttaccatg aaggcacact tgaaaatgtt acctgtaaat
    10441 atgcaaacag gccaattgaa cttgaagcca atgttgacgt agttcgccag gtgttattta
    10501 aagagcttga taaaagaagc aggttagagt attaactata ttttagggta gtggctatga
    10561 aaataacaca gcatgtgtac caggtgggag gttctaacct tacgcatcca tcagatgccg
    10621 caatctactg tattactgat ggtaaggtta gcgcactgat agatgctggc accggtgatg
    10681 gtcatgacag gcttataaaa aatttagacc gtgaacatat tgctgcctca tcaatacagt
    10741 atttattctt aacacattgc cactttgacc atgttggcgg tgcgcacaaa gtacggggta
    10801 ctttcaattg taaggttgtg gctcacgagc ttgatgcaca ctatattgaa acaggcgatc
    10861 agtatgtaac tgcagcaagc tggtacggta gctttttaac cccatgcaca gttgatataa
    10921 aggtaaacga aaagcaaaag acttttgagg tcggaagcct taatgttact atgtatcata
    10981 ttcccgggca ttcaccaggt tcaacggctt tgacagttga atctgaaggc aagctggtgc
    11041 tctttgggca ggatgtgcac gggccattaa atgatgtgtt aaaatctaac agggatgatt
    11101 atatacagtc actggagttt ttgctttcgc ttaatgctga tattttatgt gaaggccatt
    11161 ttggtgtgta ctatgaaaaa gaagaagtga aggattttat agagtcattt ttgtgaatat
    11221 aattatatta tgcaggcgaa caaacgtttg ctcgcctgca taatgtatca tcttactcta
    11281 acaacgcacc ggtaaagatg tctttgcttg ccttgataac cgcagtttca ttaaacagca
    11341 gcgcatcaat tcggccaaag aattttggaa attcagcacc caggtaaaac tgtgatgata
    11401 acacacgccc ggaatagtat gctgcttctt cattgtcagc aaggaatttg tcacgctctt
    11461 cacctttttt gtcaccaaca agctctttca tctttggtat ggtcaatgtc agtgaccaca
    11521 gatgtaacca tgcaagcgtc agcatgaaca tagcctgctg cagtggtgta caggcagcaa
    11581 agatgtgtaa aaatcttcct gcagccatct ggtctttcaa catggtgatg acttcatcta
    11641 acttctggat tcccttttca accagttcaa tgtatttatc ttcaactatg cctttggctt
    11701 tggcaatagt ttcagccatt cgtttcttca tgatattgta gttgtactgc tcaggattca
    11761 tcagaatttt acgcatggtt aggtccatgg actggatgcc gttggtaccc tcgtaaatct
    11821 gggttatctt ggcatcacgc atgtactgct caacggggta atcagaagta tatccatatc
    11881 caccatagac ctgtatagct tctgaggtaa ccagcacagc agtgtcagta cagccagctt
    11941 ttgcaatggg gataagtatc tccaccatac cctgggcttc cttctttgca tcgccgtcaa
    12001 gtacttcaat cagtgttgca ttatgtgcca gataatagtt gagcatacgc atgccttcca
    12061 cataagcttt catccacaaa agcatgcgtt taacatcagg atgttcaata atagctacct
    12121 tgggggcttc aggattgagc atttgcgtta catgaacacc ctgtatacgg ttacgtgcgt
    12181 atgtgactgc atgcatgtag gctgttgttg aaagtgccag cccctggcag gcaacgccta
    12241 atcgtgcttc attcatcatg tggaacatga tcttcatacc ctgtcgtggt tgccctaaca
    12301 accacccaac acatttgcca ttatcgccaa agttcaatgt acaggtagca gaaccatgga
    12361 tacccatctt gtgctcaata cccgaacata tgacatcatt aaactcacct aaactgccat
    12421 caggtttcac tctgtatttt ggtactataa atattgaaat gcctttggtt ccgggtggat
    12481 cgccttcaat gcgggcaaga actggatgaa taatgttttc atagaagtca ttgtcgcctg
    12541 aggttatgaa aattttctgt ccctgaatga gataggtacc atcaggctga gggatagctt
    12601 ttgttttcag attgcccaca tcagagccag cttcaggttc ggtaaggcac atggtgccac
    12661 cccattgtcc ggtgagcatt ttttccagat acatttgttt ctgttcttca gtaccaaaat
    12721 ttttgatgag tgctgctgca ccatgagaca ggccaggata tgttgtgaat gataaactgg
    12781 ctgatgtaaa aatttcactg catgccattg caactgcacc gggcataccc ataccaccaa
    12841 tttcgggatc ttcaatcatg ccaacaaaac cagcttcatt atattttctt aaagcgtttt
    12901 tgtatccttc cggaatcatt acctttttgg tttcagggtc atatttgaca ccggttttat
    12961 cagcctcctt gtttaccgaa tacacttctt caaccgccat ttttaatgct aagtcaatgg
    13021 tggcttcata catatccttg tcctgatcag caaacttttg atactgtttt aaaccatcaa
    13081 tttttaatag ttcaaataaa acaaactgga catcacgttg atctacaagt ggattgcgca
    13141 tcatatcaaa cctccggtta ttctttttaa gattaatgtt gtgctgtaat aatgttatat
    13201 tgacatacat aaaatatgca attgtgttgt caatagtaat aatattgcag ggaataaaat
    13261 tttttgcaca ttcaggttta aagtgaaacg ttcagggtaa agatgtaatt ggcaggtaaa
    13321 agaagataaa tgaccatatt ttttaaatta tattatgcag gcaagcaaac atttgctcgc
    13381 ctgcacaatg tatcatctta ctctaacaag gcaccggtaa agatgtcttt gcttgccttg
    13441 ataaccgcag tttcattaaa cagcagcgca tcaattcggc caaagaattt tggaaattca
    13501 gcacccaggt aaaactgtga tgataacaca cgcccggaat agtatgctgc ttcttcattg
    13561 tcagcaagga atttgtcacg ctcttcacct tttttgtcac caacaagctc tttcatcttt
    13621 ggtatggtca atgtcagtga ccacagatgt aaccatgcaa gcgtcagcat gaacatagcc
    13681 tgctgcagtg gtgtacaggc agcaaagatg tgtaaaaatc ttcctgcagc catctggtct
    13741 ttcaacatgg tgatgacttc atctaacttc tggattccct tttcaaccag ttcaatgtat
    13801 ttatcttcaa ctatgccttt ggctttggca atagtttcag ccattcgttt cttcatgata
    13861 ttgtagttgt actgctcagg attcatcaga attttacgca tggttaggtc catggactgg
    13921 atgccgttgg taccctcgta aatctgggtt atcttggcat cacgcatgta ctgctcaacg
    13981 gggtaatcag aagtatatcc atatccacca tagacctgta tagcttctga ggtaaccagc
    14041 acagcagtgt cagtacagcc agcttttgca atggggataa gtatctccac cataccctgg
    14101 gcttccttct ttgcatcgcc gtcaagtact tcaatcagtg ttgcattatg tgccagataa
    14161 tagttgagca tacgcatgcc ttccacataa gctttcatcc acaaaagcat gcgtttaaca
    14221 tcaggatgtt caataatagc taccttgggg gcttcaggat tgagcatttg cgttacatga
    14281 acaccctgta tacggttacg tgcgtatgtg actgcatgca tgtaggctgt tgttgaaagt
    14341 gccagcccct ggcaggcaac gcctaatcgt gcttcattca tcatgtggaa catgatcttc
    14401 ataccctgtc gtggttgccc taacaaccac ccaacacatt tgccattatc gccaaagttc
    14461 aatgtacagg tagcagaacc atggataccc atcttgtgct caatacccga acatatgaca
    14521 tcattaaact cacctaaact gccatcaggt ttcactctgt attttggtac tataaatatt
    14581 gaaatgcctt tggttccggg tggatcgcct tcaatgcggg caagaactgg atgaataatg
    14641 ttttcataga agtcattgtc gcctgaggtt atgaaaattt tctgtccctg aatgagatag
    14701 gtaccatcag gctgagggat agcttttgtt ttcagattgc ccacatcaga gccagcttca
    14761 ggttcggtaa ggcacatggt gccaccccat tgtccggtga gcattttttc cagatacatt
    14821 tgtttctgtt cttcagaacc aaaatttttg atgagtgctg ctgcaccatg agcaagggaa
    14881 ggatatgatg taaatgcaag gcttgctgca gtgaaaatct cattacacgc tattgctatg
    14941 gtaaaaggca tacccatacc accaatttca gcctcttcca ttatgccgac aaatccggct
    15001 tcattatatt ttttcaatgg ggctttgagt aaatcaggaa taattacttt ttttgcctca
    15061 gggttgtatt taacaccaat tttgtctgct tccctgttaa caggatatac ttcttctact
    15121 gccatcctga atgccaaatc gatggttgcc tcatatatat ccttatcatg atcagcaaac
    15181 cgtggtattt tttttaactc atccagtttt agtaattcaa agagtgcaaa atgtacatcc
    15241 ctttgatctg ccagttgatt acgcattgat acagaaatca tactataccc ccactattag
    15301 tattgttaaa atgattgaaa aataaaagta taacattatt aataacatat atatacatgt
    15361 catactattt tgtcaatgta tatattattt gtaagtaaaa ttttgtatat tcaataataa
    15421 tgatattatt taaaaataat tttatgaaca taataaaaaa taataattac tcttgtgctc
    15481 atggaagcag gtattgttac attatcttat tttaaagggt atttactcat tatcgcgata
    15541 gtattaccaa aaatgatgaa aataagacga taattattat tcgcattgta gtgattgctc
    15601 tgagtggttc gacaagctca ccaaccttgg ttcgacaagc tcaccaacca tggttcaaca
    15661 ggcggtccct gagcccagtc gaagggcggt ccctgagcat gtcgaagggt ggtccctgag
    15721 cccagtcgaa gtgattagca accgtttata gaaatgtaga gaaattgttc attgtaaaat
    15781 ccatcctctt tgtggtggga ggtgaaatag atcatatcga gggtataatc tgtattgaat
    15841 atacatgtgc aggcactcac aggcctaaag aaactgcatt ctatgggggt caacgcttaa
    15901 tgttgtgaag ttgagaagat atatattggt tactttatta tagtaactta tatttaaaca
    15961 gcaacaggat aaccaaccat agtataataa cagtaataga gatatagaac aaagtatatg
    16021 ccatggttat cccccctatc ccaaggagag ggatgaacat actggtgata attcctcctc
    16081 ccataaatgg ttcatgcaaa agctgcttgt atgcaaatgc ttctaatgca ggtgtcctgt
    16141 aatctggatc aacaattcta agcagcataa ggccggtagc tgttactccc attgattgtc
    16201 ccatttcagc aatggctcgt tcaaaccagc tatcgggaag taaccgtttt gctaaaacta
    16261 acaggcaaaa aatattccac agtattcccc caatcaccat gatagtaaaa ggcactatcc
    16321 cctgaataac aataacaacc tgaatcatag caactgcgct cactataaga atatccagcg
    16381 ccatattctg aattcgccgc accagtccaa gatcaatagt ttcatgcctg tcaaaatgct
    16441 gtgcaaaaag ctgtactata aggccgccaa tcatacacag gggaaaaagt ggaaagcttt
    16501 ttatgatact aataatgttg gtatatgttg catgtgacag aacagtctgt aagcaatata
    16561 tgccagcaac taatccttct ttaaggaaat aaccaatgcc aacagcaatg ccaacaattg
    16621 ccacatgata cgacattgca tcaagagaat cggtatggat agagaggtat cctgcaatag
    16681 gtctgcggtc gacggggatt atacctatgg tttcatcttc agggatattt tctacctgca
    16741 gggaacggtg agtatagcct tttaggtttg cccagttaat aagtgcaatt ccagcaataa
    16801 cagcacttat aatgccaact gttgcacttg ccagtgcaaa gtccttgcct tgtggccact
    16861 tcatcatttc aaatgttggt gccagtccgc ttgctgtgcc atggccgcct tcaaaaccca
    16921 cttcaataac agctccaaac atggcaggca ggttccacac tttgctgatg ataaaaataa
    16981 atagccccaa tcctaccata tattgtcccc aggcaactat ctggccatac gacagttggg
    17041 ggattgattt ttttaaaact attttaaacg atggcaatga aacaccaata aaaagacagg
    17101 caaagactat gttaataagg aaagagggca acatggcaag tccatcaaac caggtggtaa
    17161 gagtattgat agatagtgtt ttagctgtct gatacaccat caatccaacg atgccgccta
    17221 ataacgatga tggcaggtat agcttctgga aaagtttaat gtgcagcctg agatagtgtc
    17281 caacaaatag caggatactg atagttataa acggtaacag tagtttcatg cacaccttct
    17341 tgatttgtga tatggggcaa tttactcatc tttatttttt atgcaagcaa ggtcaagata
    17401 aattatactg ttaccatgtc tttattttta tgcggcgata gttacttgtg attagtctgc
    17461 agtgcacaac catgtgcagg caaaaattta ttagcagtaa ctatcgccca aaaaattgta
    17521 tttcaggata caatgggctc acccttgcag agcatcattt gcgcctgtgg cgaatacatg
    17581 catcccagtg gctttttgcc atctaagcct aagtcaagca gattctgtgc catgctgtga
    17641 ttgctctgtg cattcactgt gcattttcca gaaaagccgt aggctatttt gaaacgcgtg
    17701 tcaaccacag tca
//
LOCUS       DAORYL010000064        17653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_2635_length_17653_cov_14.524832, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000064 DAORYL010000000
VERSION     DAORYL010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 17653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17653
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2635_length_17653_cov_14.524832"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..742
                     /locus_tag="PLV81_11195"
     CDS             41..742
                     /locus_tag="PLV81_11195"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM10703.1"
                     /translation="MKFKLFLLLVASVAIALSYLYAPQVYRYYLTVYYHKILKMDQAA
                     FVKKIEDAYKNNDYATVQRYCDHGSMLYPANKKIKKYTGLYLLSQDNVKGAFILIALD
                     DIPNDTRHLEKIISTLDTNRSYKEVIMVVSKKGARTPLMQFRYGKALFFMGDDRQALI
                     VLKQSYGTGIRESDYFIGASYHHLKDYNNALVWYKKALSREPSNQEYIKAIASLYKQM
                     GDYPEATQYIMRLQQ"
     gene            758..2152
                     /locus_tag="PLV81_11200"
     CDS             758..2152
                     /locus_tag="PLV81_11200"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOM10704.1"
                     /translation="MKHELDYFFHPDGVAVLGASENQRTGGFHILRNVLGGYTGRVYP
                     VNPRYTELLGVKCYPDIQSIPDTFECLIYFIPARFILDTIKEAAKKGVRAIIIESAGF
                     AEVGSQGQALQQEAVALANSLGIRLWGPNCMGYLDGHTRNVFSFMYTDKWKTLMKPGD
                     VSLIVQSGMLSAGFLMSVLERDNIGISKVCSIGNKCDVNEIDLLDYFCGDSETGVVGC
                     YLESIVDGKKFLQIVKNTQKPIVVLKSGRSEQGKKAAMSHTASLAGNTAVQAGAFKQA
                     GVVQVIDLYELIDTVKAFSYIKNYRPDKGVALMTFSGGAGIVTTDLMADYSIPLANLS
                     NDTLQAVQKLYPSWMGASHPLDLWPAVEQNGLDAVYTATVEALMSDTSVDSIIVECFA
                     SRYYDPAFFKDIGNMMRTHKKPVVLWLVGEKDVVDNYKMHAEGSGIPVFGEIGRCIQI
                     IQALRFHFSKRGKM"
     gene            complement(2335..3324)
                     /locus_tag="PLV81_11205"
     CDS             complement(2335..3324)
                     /locus_tag="PLV81_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10705.1"
                     /translation="MKFLTKVCFIVLVLFASTTVFAAWVYVPMSINAQKGDIVLSTSP
                     GFIMDLLAILGCYWSHSGMAVDNGYNIRHNTMYVSQIPIEYNYIWFIKTTPKRLDPTR
                     LSNGLPGILTEDIDTAYNTTLNFHAAGGAVLKPTVANEALYRQYLNAAADVFNYLKAY
                     YRVHAYVNMYQLDYANYYITGRGNHCSGTCWYANYFAGKTMAVATIPPALVTQCANSL
                     YTSVKNMVRDEAGGFGAFIIDIEGLFGTGADEKIANQIVNTFGFDRSTDTSSYWRNYI
                     NQVTATANAPDHLLLSSYTNPSGHNVGVQTTSTSYYGQVEPLVITDGYYIEVP"
     gene            3677..4393
                     /locus_tag="PLV81_11210"
     CDS             3677..4393
                     /locus_tag="PLV81_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10706.1"
                     /translation="MRNFASILTFVIAIVVFASSCATLNTQDTKLVDQLKAENRVCSQ
                     NLNLFMRENDVLKAENAKYKKENVNLQSRINLLQSDLNALQTKYKNDIAMWNQRYDSL
                     QQKYDVFVKQSDVKLQELDQRNKELEKKMADEIARLNEVIRKQEVDFIKEKENIINQN
                     AKKEIEYQATIDSLQKQVTSLTSEKEQLKSKLAEAEVLIQKIQKELDEMKKSSITEIK
                     KAEDTGTETKKDEQKKEESK"
     gene            4439..6499
                     /locus_tag="PLV81_11215"
     CDS             4439..6499
                     /locus_tag="PLV81_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10707.1"
                     /translation="MAFFSGAFAYDTLTIIATSNLKGNFSLDMDDGTDPLLSVARGII
                     AERASKQVDCYIDLGNAFSPGVLSKYSYGAVMLDYFEFLQCDAVLIGSHDIAVGLRNL
                     ELASRSRSTQLLSLNITVDNKPIFKPYWVYKKNDISIGFVGITSSKGIFDIAEKSMLS
                     ISLAGYAEYLTSTIVALKGMGCKYIIILSGLSADQNMLLMKEYPDINLILAGGDAAGL
                     YYDVYAPRVDLEDGRSVITLSKSNGHYTIKLGLKDNLVCETIRFSSADKKVSDSSYNE
                     LVERVNLWKERYMEIGTATLADNFPATNVSSVTIAHLLRHYYNAEVAVVTVSDIFATT
                     LSGNVTSIDVQRIIQNDYTIFTYRLSGKQIQTISSASSLIFSGLSAGKIQGVAVDPDR
                     MYRVVSTQSAYDTIAAIVGEVSYTNTWTPLSSVITNDIQGNKNIAKDDFSYLDNRFRL
                     TVDINLSNIYDNSVITTDDTASTPPGMPSQTYKKWGIEDSADITVYNQYHRLTVTPYI
                     YYMRQDELYLQNLLRGTLFYTFTFNPYINPYHKSQVDTVVVEKEDGLRPILFRETVGA
                     SFVYSYFSGRLGFGFEKQTQDPEKDLLYGAESIFQIQIPFWDYFTYTFNVDNFISAPE
                     GGDSSYQIRTDIKNAVSIKINTWLSLTIRHRWFYYNPLDTQIPYTYTQLLISLDVKAK
                     FKVF"
     gene            6746..8647
                     /locus_tag="PLV81_11220"
     CDS             6746..8647
                     /locus_tag="PLV81_11220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOM10708.1"
                     /translation="MSSLIVLLLNRALRDQISEVFTQKGFQLSLVERDECSKESINKS
                     TPDIVLFDVGMHSEDDFLLYLDVVRWSEVPVLPVIYRKENSHCTMLKAHELLKDVEAI
                     LLGTDYSDQKDNLAAVQQYPYALTHYHDIFETIPYAIFIHDIHTGEVLDVNVAATELF
                     GYSRDELMYENPAVAYPHNNEFTLSNAMKHIDVAASKGAHTFEWCAYKKSGQVMWLKI
                     HLTLVSISGSKQIMAVVHDITRERQLQKALEASEKRFKSVFEEFSDGLMIIDKEGIID
                     CNTAVEVMFGYSKDELIGLHPAQLSPQKQPDGSDSRMLSNTKIQEALQNKTAPFRWLH
                     RRKNGQDFWTEVTLVLITLDGVNVILSIVKDISDIVQYEYDREQLQQHIYQLQKMEAI
                     GTLTNGIAHDFNNMLTSIIGNLNLASIYMEKKDFDYTKELHHTIESAIISASQAADLV
                     KKLLTVSRKTKGNVEKVLIQEVINDVKTICTSSFPKIVHVFYNIDKEPVYVNIDRVLL
                     TQALINCCINASHAVTLMRSDGREGGTITVECTKSTCPEHICAVHPEANSDAVYAVIS
                     ISDDGVGMDEETRQRIFEPFFSTKDKTIGTGLGLSIVYSTITNAGGFIHVDSVPNKGT
                     TISLYLPAV"
     gene            complement(9047..9844)
                     /locus_tag="PLV81_11225"
     CDS             complement(9047..9844)
                     /locus_tag="PLV81_11225"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOM10709.1"
                     /translation="MKGYIKAAYIYWLLPQPVKSRCLTALDSKQQATLNKGLQHITSL
                     SIQQEISILKEAIDIINAIKIHTKHIRDISIIASCIIFGIIIILSALVSSGWHVVDSM
                     YYILQYGGLHAVLVPFIMMYAGKLMRKPFFQLVKPSHIILDILMAIIAATAIVIIFSF
                     IFPHNTVPVTKLSIIASIFAITAVPLSEELFFRGIIFLHGGKHYGYTASWFFASFVFA
                     TIHLPNTPMEFAAYFVFSSMLCFVAYRFSLFASIITHMLSNGILFIL"
     gene            10113..11201
                     /locus_tag="PLV81_11230"
     CDS             10113..11201
                     /locus_tag="PLV81_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10710.1"
                     /translation="MAEQIRTITDKGQFPKIFSLFFANNDVFLRTASGDLKIVYMGFA
                     DGTVAFKIPYVKNMPDSCVILTRKDSYIVNGVFKLKEKDADDMYVFYPVQFQIISSPR
                     KEERIELGTGKSVLFITNLISDYILQNSLALSLKKVDNVKEILRFDLEKRFPYVKIYF
                     LNEGMADPRMKYFYANPVPYFIPSMNAQPDAKGQKMFNTYVNEIYSKDYMLQNRKNLI
                     AEIAVPVLYHGKIPYGYIQVNSDKPLTDGMFSVIKRLGVVADELFKKNKLFIPETDRL
                     LIADASFSGIGIVFRERKYIRYFKEGSYVIGDILLPNGARANVLCVVRNITLMENKII
                     KVGCQIKDIDALSEVHYHEYLESAGVID"
     gene            11287..11781
                     /locus_tag="PLV81_11235"
     CDS             11287..11781
                     /locus_tag="PLV81_11235"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HOM10711.1"
                     /translation="MNIKDLQSIILQEIDIYSTLVQLEEQKKEAITAFNGDLLFSLSK
                     DQETLILNLEKLEKSRQQFIKTHVTGHAFQPHDMTLKDFITLTRMHDNENLIVQAQKL
                     KETIEKFARLHEANKKLIHDNIEFFNVILTSLRKAVTIETGYGPEGLAKRSASGSVLM
                     NKTV"
     gene            11790..13700
                     /gene="flgK"
                     /locus_tag="PLV81_11240"
     CDS             11790..13700
                     /gene="flgK"
                     /locus_tag="PLV81_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004765678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOM10712.1"
                     /translation="MNSTFMGIEIGKKGLLAHQQALHVTGHNISNAANEEYSRQRVVI
                     TASDPLYVPALNRANQPGNIGQGSEVAMIQRIRDEFIDDRIMVEKQAFGYWKARNDFI
                     YQIEMVYNEPSDQSIRSRLDALWEGFQELSKYPEERSTREVVKEKAIALANEVKYTYG
                     KLYELQKQANRQIGHTVEQINEYAQTIRDLNERILKAEALGDNPNDLKDKRDALIEKL
                     SQLVNINVSRTDKDELIVYIGGENLVQGEVFRPLAAIEDPDNNGMYKVLWKQTLTDVN
                     IQSGELAGLISIRDGVLRQNINDVNAFAINLTDLINEVHRDGFGKNNQTNNNFFKHIA
                     VSDNVEGNFDLNNDGINDVTALFKISGNNKVDASAAIGITGTLTFVKNNALDQEIKIN
                     YYATDTLLDVIKRVNDAKIGVVGYINHNSQLAFKATIAEDTDKKNFIIRHLEDSGQLL
                     VGYAGILKESGPQGAFDYRRVDDIRKIIASREHITITPMFNPASYMDIDDAIKYDIDS
                     IAAAKGKDLGGTGDYNTSNGVGDGSNALALAALKHKHAMIDSNATFNDFYTSLISRIG
                     SQGEEAKDRIASQETLLKNLANLRESVSGINLDEEMANMVQFQHGYNASARVIAMIDR
                     MLETIIKLGQGV"
     gene            13704..14966
                     /locus_tag="PLV81_11245"
     CDS             13704..14966
                     /locus_tag="PLV81_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HOM10713.1"
                     /translation="MQRVTNQMINNTMIYNLNKHQQEMDKMQDMLATGKNVQFPRDNP
                     VAATNQMLYKTALVEIEQYIKNIDESKSKLSEVDSALQSVIRIFQRLRVLAVQGAHGI
                     YTSFERKEAAATEINQMLEELVNIANIKGATGKPIFGGYQTGTENIPDPFVPIYQTLT
                     AGNQGNAMIGVQYRGNIGKILREVANSEYLDVNVPGNWAFWATNQVLSANKDVSQYRA
                     LATQSFKIDGVSIQVAAGDTIDMVIDKINNAGLSVKASIGARNNLVLETTVPHAIWLE
                     DEGSGTFLRDAGMINPDFPNPPNNIDPTVSVSGMSIFDMVIQLRDDLVRGDQELVGGR
                     DLGLIDMALDNVLRHIAAVGAKENRIEELAKRSEYAKSNILDILAKSEGIDFPETIMN
                     FRWLESVHNYALAVGARSIKPTLMDFLR"
     gene            14991..15443
                     /gene="fliW"
                     /locus_tag="PLV81_11250"
     CDS             14991..15443
                     /gene="fliW"
                     /locus_tag="PLV81_11250"
                     /inference="COORDINATES: protein motif:HMM:NF009793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HOM10714.1"
                     /translation="MVTIATKPFGQIEVDERQIIDFPEGIYGFEDIKKFVILDANEKS
                     PFKWLQAYDEPDLAFVIIRPIDFMIQYELEVMQEDLEDIGAKSPDEVIVFAIVTIPED
                     PSRMTANLQGPVLINPKTRKGKQAISLSDKYTVRHYILEEIKKAQVKG"
     gene            15448..15714
                     /gene="csrA"
                     /locus_tag="PLV81_11255"
     CDS             15448..15714
                     /gene="csrA"
                     /locus_tag="PLV81_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HOM10715.1"
                     /translation="MLVLARKVNESIMIGDDIEIVIIDIKGDQVKLGIKAPKSIAIHR
                     KEIYEEIQKENIAAMQSQLKPDTLKDITDILQTHKEKNIDNSKK"
     gene            15769..16437
                     /locus_tag="PLV81_11260"
     CDS             15769..16437
                     /locus_tag="PLV81_11260"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HOM10716.1"
                     /translation="MPDIIVQTNVAYLVAIFFIIFIIREIVAFKKILKLKYIFTPLIT
                     YIVIIIALYLSYVRGFTVYNIFIIAGLVFALIADTFLMIEEVSFFIHGLFFFLLTHLC
                     YVYALSINYHFGIIDVIIMLVILAIFFLYYLVIHKAKGTVYVPVILYMLVLSLVLMLA
                     VGTCIKNEYPRGAIVTIAAILFAISDGVLAFNQFVHRIPHSTVVTWSLYAPAQLLFAV
                     SCYY"
     gene            16604..>17653
                     /locus_tag="PLV81_11265"
     CDS             16604..>17653
                     /locus_tag="PLV81_11265"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM10717.1"
                     /translation="MKKILIFVLISLLSCGQLIQQSPQKDYELIYKNLTNAESLENPV
                     QKDKLINKAYTLLMQRQRELLKEKKSDPPVAALLAYYYFLKGNYQDASAQVELAQSSV
                     SGNNPLGVIIQARVLLATKGKAGAQNALDIVKSLTVTESPMAYIVTGDAYFLKGDYDK
                     ARDNYTKALLLNRELQVVAANRLEVISRIKTLSINVAKVSDFILEPTMTREKLAYLMY
                     EIFELQKYIAAGKPFDANFIDLEKSQYKNAIISLRGKGFFSYIQGSVFEPFMVVSRGE
                     MAKIVEDFLVLTRNNEGLRSKFKNESPSFFNDIKNDNEYYNAMRLAVDMGIMSASLSQ
                     DAYPDESVTGLQAIMI"
BASE COUNT         5674 a         3067 c         3757 g         5155 t
ORIGIN      
        1 cttcaaagaa tttgccaagt taatgattgg taattaaact atgaaattta agttattttt
       61 actgcttgtt gcatccgttg caatagcact ttcgtacctg tatgcgccgc aagtttacag
      121 gtactatctc acagtatact atcataaaat ccttaaaatg gatcaagcag catttgtgaa
      181 aaaaatagaa gatgcctata aaaacaacga ttatgccaca gtacaacggt attgcgacca
      241 tggcagcatg ctgtatcctg caaataaaaa aattaaaaaa tataccggat tgtatttact
      301 atcgcaggat aatgtaaaag gtgcgtttat ccttattgcg ttagatgata tccccaatga
      361 tacccggcat ttagaaaaaa taattagcac tcttgataca aacaggtcgt ataaagaagt
      421 tattatggtg gtaagcaaaa aaggtgcacg cactccatta atgcagtttc gttatggaaa
      481 agcacttttt tttatgggtg atgacaggca ggctttgata gttttaaaac agtcctatgg
      541 caccggaata cgtgaatcgg attactttat tggggcaagc tatcaccacc tgaaagatta
      601 caacaatgct cttgtatggt ataaaaaagc attaagccgt gaaccttcca atcaggaata
      661 tattaaggca atagcctcac tgtataaaca gatgggtgat tatccagaag caacacagta
      721 tatcatgcgc ttgcagcaat agttaacgga ggaagttatg aagcatgagt tagattattt
      781 ttttcatccc gatggtgtag ctgtactggg ggcaagtgaa aatcagagaa ccggtggttt
      841 tcatatcctg cgaaatgtgc tgggtggata taccgggaga gtgtatcctg ttaatccacg
      901 atataccgaa ctattaggtg tgaaatgtta tccggatata caatccattc ctgatacatt
      961 tgaatgttta atttacttta tccctgcacg gtttattctg gataccatta aagaagcagc
     1021 aaaaaaaggg gtcagagcaa taataattga gtcagctggc tttgctgagg ttggcagcca
     1081 ggggcaggcg ctgcaacagg aggcggtggc acttgcaaat agcctgggga tacggctgtg
     1141 gggtccaaac tgcatggggt atcttgatgg tcatacccgc aatgtctttt catttatgta
     1201 cactgacaaa tggaaaacat taatgaagcc gggagacgtg tcgctcattg tgcagagtgg
     1261 catgctgtca gctggctttt taatgtcggt actggagcgc gacaacattg gcataagcaa
     1321 ggtgtgttct attggcaaca agtgtgatgt caatgaaata gatttgcttg attatttttg
     1381 tggcgatagt gaaacgggag tggtgggttg ctatttagaa tcaattgttg atggtaaaaa
     1441 atttttgcag attgtgaaaa acacgcaaaa gccgatagtt gttttaaaat caggacgaag
     1501 tgagcagggt aaaaaggctg caatgagcca cacagccagt ttagccggta atacagcggt
     1561 acaggctggt gcgtttaaac aggctggcgt tgtgcaggtg attgacctgt atgaacttat
     1621 tgataccgta aaagcgtttt catatataaa gaactatcgc cctgataaag gggttgcgct
     1681 tatgacattt tccggaggtg caggcattgt cacaaccgat ttgatggctg attattctat
     1741 tcctcttgca aacctttcca atgatacact gcaagcagtg caaaaattat atccttcatg
     1801 gatgggggcc tcccacccac ttgatctgtg gcctgctgtt gaacagaatg gacttgatgc
     1861 agtgtatacc gctaccgtag aagcattaat gagtgataca tctgttgatt caatcatcgt
     1921 tgaatgtttt gcaagccgtt attatgatcc tgcttttttt aaggatattg gtaatatgat
     1981 gcgtacccat aagaagccag tggtgttatg gcttgttggt gaaaaggatg tggttgacaa
     2041 ttataagatg catgctgaag gttcaggtat tcctgtattt ggtgaaattg gcaggtgtat
     2101 tcagattata caggctttac ggtttcattt cagcaagcgc gggaaaatgt gaaaaggggg
     2161 taccccccaa gtcgtttcga tacaaatttc gcaacgctca ttgagtaatt ttgccagatt
     2221 ttgtggcaaa attgtatcga aatgagcgag tattacgata taatgtttgc aaaatttact
     2281 caacgacctc ttttgggaca ccccctttta attgtactta tggataaatc actattaagg
     2341 cacttcaatg taatagccat ctgtaatgac aagcggttca acctggccat agtatgaggt
     2401 agaagttgtc tgtacaccta cattatgccc tgatggattt gtatatgatg acagtaataa
     2461 atggtcaggg gcattagctg tagctgttac ctgattaata taatttctcc agtatgaaga
     2521 tgtatcggtt gacctgtcaa aaccaaacgt attgacaatc tggttggcta ttttttcatc
     2581 ggctcctgta ccaaataaac cttctatatc aatgataaat gcaccaaatc ctccagcctc
     2641 atcacgaacc atatttttaa ctgatgtata gagtgaattg gcacattgtg taacaagtgc
     2701 tggtggtatt gtagctacag ccattgtctt gccggcaaaa tagttagcat accagcatgt
     2761 acccgaacaa tggttgccac ggccagtaat gtagtagttg gcatagtcaa gctgatacat
     2821 attgacatat gcatgtacac ggtagtaagc tttgaggtaa ttgaaaacat ctgcagcagc
     2881 attcaggtat tgcctgtaca atgcttcatt tgcaacagtg ggtttaagta cagcgccacc
     2941 cgcagcatgg aaattcagcg ttgtattata cgcggtatca atatcttcag tcagaatacc
     3001 aggaagccca ttgcttaatc ttgttggatc aaggcgtttt ggtgttgttt tgataaacca
     3061 gatgtagtta tactctatcg gtatctggct gacatacatg gtattatggc ggatattata
     3121 tccattatca acagccattc cgctatggct ccagtaacaa cccagaatag caagcaggtc
     3181 cataataaac ccggggctgg ttgaaaggac aatatcgcct ttttgagcat taatgctcat
     3241 gggcacataa acccatgctg caaatactgt tgtgctggca aagagcacaa gcacaataaa
     3301 acaaaccttt gttaaaaatt tcatagaacc cccatataac atttaagtgt tattcaccgt
     3361 gagataggaa ataggatcgt ttgcaaaatg agtgattatt cactcagtgc ggtgtaattt
     3421 gctgtatcta atgctataag ctaaaaattg tcaactaaaa aaataccata tagtcggtat
     3481 tttattaaga tttttaattt tggggtcaga gccttaaaaa tccttttatt tggaataatt
     3541 ttttatacag taacaaaaaa agattgaatc tcactaaagt ttgcttaaga ttactgtatt
     3601 acttatgcta agtaaaattc tttttttaac cggtagggta tacatactat atttatcagg
     3661 caggtggggg ctattaatga gaaattttgc aagcatttta acctttgtca ttgctattgt
     3721 tgtttttgca tcttcatgcg ctacgctcaa tacccaggat acaaaacttg tggatcaact
     3781 gaaagctgaa aacagggttt gttcgcagaa cctgaacctc ttcatgcgtg aaaatgatgt
     3841 gctgaaagct gaaaacgcaa aatataaaaa ggagaacgta aatctgcaat caaggataaa
     3901 tctgttgcag tcagacctca atgcgttgca gacaaaatat aaaaatgata ttgcaatgtg
     3961 gaatcagcgg tatgattcac tacagcaaaa atatgatgtt tttgtaaagc agagtgatgt
     4021 taaattgcag gaactggatc agcgcaataa agaacttgaa aaaaagatgg cagatgaaat
     4081 agcgcggtta aatgaagtta tccgcaaaca ggaagttgat tttatcaaag aaaaagaaaa
     4141 tatcataaac cagaatgcaa aaaaagagat agaataccag gctactatcg atagtttaca
     4201 gaagcaggtg acatcattaa cgtcggaaaa agagcagctt aaaagtaaat tagctgaggc
     4261 cgaggtgctc attcaaaaaa tccagaaaga actggatgag atgaaaaaat ccagtataac
     4321 agaaattaag aaggctgaag atactgggac tgaaacaaag aaagacgagc aaaaaaaaga
     4381 agaaagcaaa taaacaatgc gttttggtta ttacatcatt acaacatgca tttttgtaat
     4441 ggcatttttt tccggtgcat ttgcgtatga tacgctcact atcattgcaa caagcaatct
     4501 taagggaaat ttttcactgg atatggatga tggtactgat ccgctattat cagttgcccg
     4561 cggcatcatt gcagaacgtg catctaaaca ggttgattgc tatattgatt taggcaatgc
     4621 attttcacca ggtgtgctgt ctaagtacag ttatggtgct gtaatgcttg attattttga
     4681 atttttacag tgtgatgctg tgctgattgg ttcacacgat attgctgttg gattacgtaa
     4741 cctggaactg gcatcacgca gccgatcaac acaacttcta tcgcttaata ttacagtaga
     4801 caataaacca attttcaagc catactgggt atacaaaaaa aatgatatat caattggctt
     4861 tgttggtatt acttcatcaa aaggcatatt tgacattgca gagaaaagca tgttaagcat
     4921 ttcgcttgca ggatatgctg agtatcttac cagcaccatt gtggcactca aaggaatggg
     4981 gtgtaaatac attattatcc tgtcaggcct ttcagcagat caaaacatgc ttctcatgaa
     5041 agaatatcct gatataaacc ttatactggc cgggggcgat gcagcaggtc tgtactatga
     5101 tgtctatgca ccacgtgttg acctggaaga tggacgttcg gtgattacac tttcaaaatc
     5161 aaatggtcat tataccatca aacttggatt gaaagataac cttgtttgtg aaaccatacg
     5221 tttttccagt gcagataaaa aggtaagcga tagctcctac aatgaactgg ttgaacgggt
     5281 caatctgtgg aaagagcggt atatggaaat tggaacagca acactggctg acaatttccc
     5341 ggcaaccaat gtatcatcag taactatcgc ccatttgctg cgccactact ataatgctga
     5401 ggttgctgtg gttactgtga gcgatatatt tgcaacaacg ttaagcggga acgttaccag
     5461 cattgatgtg cagcggatta ttcagaacga ttacaccatt tttacctaca ggctcagtgg
     5521 taaacaaata cagacaataa gtagcgcttc ttcacttatt ttctcagggc tttctgcagg
     5581 gaaaattcag ggtgtggcgg tggatcctga caggatgtat cgtgtggttt caacgcagtc
     5641 ggcctatgat acaatagctg ctatagtggg tgaggtttcg tataccaata catggactcc
     5701 actgtcatca gtgattacca atgatataca ggggaataaa aacattgcta aagatgattt
     5761 ttcatactta gataaccgtt tcaggcttac ggttgatatt aacctttcaa atatatatga
     5821 taactccgtt attacaaccg atgatacggc ttccacacct cctggcatgc catcacaaac
     5881 atataaaaag tggggtattg aggatagtgc tgatattacg gtctataatc agtaccaccg
     5941 attgacagtc actccctata tctattacat gcggcaggat gagctatact tgcaaaattt
     6001 actgcgcggg acgctttttt atacgtttac atttaaccca tatataaatc cgtatcacaa
     6061 atctcaggtt gatacggtgg tggttgaaaa agaagacggg ttgcgcccga tattgtttcg
     6121 tgaaacggtt ggtgcctctt ttgtatatag ctatttcagt ggcaggttag gctttggatt
     6181 tgaaaagcag acgcaggatc ctgaaaaaga tttactctat ggtgctgaat ccatttttca
     6241 gatacagata ccattctggg attattttac atacaccttt aacgtagata attttatctc
     6301 ggctcctgaa ggtggcgata gttcttatca aataaggact gatataaaaa atgccgtaag
     6361 cattaaaatt aatacatggc tttcattaac aattagacac aggtggtttt actataatcc
     6421 acttgatacg caaataccat atacctatac ccagctttta ataagccttg atgtaaaagc
     6481 aaagtttaaa gtattttaac ttgttttatt cattatagtg tggtatattg ttaatggtga
     6541 tatttagcga tagtaactga atagataact ggctgcatat attattttca aatagtatgc
     6601 actcaccgta atcccacgta ttattcttcc tgacatccct aaaaaatcac tacgcaaaca
     6661 agaaatgtgc tttgagtata attttttgtt gatttaatta tttaaatttc gtaaattagg
     6721 caaatgccta aaaaaagagg tatcaatgtc aagcttaata gtattattgc taaacagagc
     6781 gctgcgtgac cagatttctg aggtttttac acagaaggga tttcaattgt ctttggtgga
     6841 acgcgatgaa tgcagtaaag aatccattaa caaaagtact cctgatattg tgttgtttga
     6901 tgtgggcatg cacagtgagg atgacttttt actgtattta gacgttgtac gctggagtga
     6961 agtgccggta ttgccggtaa tataccgtaa agaaaattcc cactgtacca tgttaaaggc
     7021 ccatgaattg ttaaaggatg ttgaagcaat tttacttgga acggattatt ctgaccagaa
     7081 agataatctt gcggcagtac agcagtatcc gtacgccctg acacattacc atgatatatt
     7141 tgaaacaata ccgtatgcaa tattcatcca tgatatacat actggtgaag tgcttgatgt
     7201 caatgtggct gctacggaat tatttgggta ttcacgggat gaattgatgt atgaaaatcc
     7261 tgccgttgct tatccacaca ataatgaatt tacactatca aatgcaatga agcatattga
     7321 tgtagctgca tcaaagggtg ctcatacctt tgagtggtgt gcgtacaaaa agagtggcca
     7381 ggtgatgtgg cttaaaatac atctgacgtt ggtttcaata tctggaagca aacagataat
     7441 ggcagtggtg cacgatatta cccgtgaacg gcagttacag aaagcactgg aagcaagtga
     7501 aaagcgattt aagtcagtat ttgaagaatt ttctgatggg ctaatgatta ttgataaaga
     7561 gggcattatt gactgcaata ccgcagttga agtaatgttt ggctattcta aagatgaatt
     7621 gattggcctt catcctgcac agctatcgcc acaaaagcag cctgatgggt cggattcacg
     7681 aatgctttcc aatacaaaga tacaggaagc tttgcaaaac aagacagcac cattcagatg
     7741 gttgcacagg cggaaaaacg gacaggattt ctggacagaa gttacactgg tattgattac
     7801 gcttgatggg gtcaatgtta tactatctat cgtcaaggat atcagcgata tagtacaata
     7861 tgaatatgac cgtgaacagt tacagcagca catatatcag ttacagaaga tggaagctat
     7921 aggaacattg accaatggta ttgcgcatga tttcaataac atgcttacca gtattattgg
     7981 caaccttaac cttgccagta tttatatgga aaaaaaagat tttgattata caaaggaact
     8041 gcatcatact attgagtcag ctataatttc agcgtcgcag gcagcggatt tagtaaaaaa
     8101 gcttttgacg gtatcacgta aaacaaaggg gaatgttgaa aaagtattga tacaggaagt
     8161 tattaatgat gtcaaaacaa tctgtaccag cagttttcca aagattgtgc atgtgtttta
     8221 taacattgat aaagagcctg tatatgtcaa tattgacagg gtactattga cacaggcact
     8281 gattaactgc tgcattaatg cttcgcatgc agttacattg atgcgaagtg atggtcgtga
     8341 aggtggcact attaccgtag agtgtacaaa gagtacctgc cctgaacata tttgcgcagt
     8401 acatcctgaa gccaacagtg atgcggtgta tgcggtaata tccatcagtg atgatggtgt
     8461 tggcatggat gaagaaacac ggcagcggat atttgagcca ttcttttcca ccaaggacaa
     8521 aactatagga acaggtcttg ggctttccat tgtatattca accattacca atgccggtgg
     8581 atttatccat gttgattctg tacccaacaa aggaactaca ataagtttat atctgcctgc
     8641 agtatagttt ttaacgcata caatacttta taataaataa tgacattcgc caggaaatga
     8701 tcgatttatg atctatttca cattgcgtca tcctgaactt gatccaggat atcaaataag
     8761 catatcaaca ctgactcgtg gctgtcccaa aagttgtttc gatacaaatt tcgcaacgct
     8821 cattgagtaa ttttgccaga ttttgtggca aaattgtatc gaaatgagcg agtattgcga
     8881 tatactgttt gcaaaattta ctcaacgacc tcttttggga cagcctccta agattaaata
     8941 tcaatttcaa aatgactata taagaataga ttctggatca agccccaaat gacgttatat
     9001 gtagattata agtccagctc atgattatgg tatagtaatc aatttattat agaataaaaa
     9061 gtataccatt tgacagcatg tgtgtaataa tgcttgcaaa caaagaaaat ctgtatgcaa
     9121 caaaacataa catactgctg aacacaaaat atgctgcaaa ttccatcggt gtgttgggca
     9181 ggtgaatggt tgcaaataca aatgaagcaa aaaaccatga agcagtatac ccgtagtgtt
     9241 tcccaccgtg caaaaagatg atccccctga aaaaaagttc ttcagaaagc ggtactgctg
     9301 ttatggcaaa tatcgatgca atgattgaaa gtttagtaac cggtactgta ttatgaggaa
     9361 aaataaagga aaagataata acaattgcag ttgctgctat gatagccatc agtatatcaa
     9421 gaattatatg tgaaggtttt accagctgaa aaaaaggttt cctcataagc ttaccagcat
     9481 acatcataat aaaaggcact agcactgcat gaagcccgcc atactggaga atataataca
     9541 tagaatcaac gacatgccaa ccggatgaaa ctaaagccga aagaatgatt atgatgccaa
     9601 agataataca tgaagcaata atgctgatat cgcgtatgtg ctttgtatga atcttgatgg
     9661 cattgattat atcaatggct tctttcagta tgctaatttc ctgttgtatt gatagtgatg
     9721 taatgtgttg taagcctttg tttaacgttg cctgttgttt tgaatccagc gctgttaaac
     9781 acctgctttt aacaggttgc ggtagaagcc aatagatata tgctgcttta atataacctt
     9841 tcatgcttac gcaattatgg agaaattatt gttattttca agcaaaaata ctcatttgac
     9901 ttttatgcgt aaagaaggta gattgattgt acagtaacgt tgctgtatat atctaattga
     9961 tatagtataa aatggaattt caaaaaattt tattattgct acaaaagaat gcgtaataaa
    10021 tctttgtaat tttcactggc atcaaaaaat taattgaaaa aacagtccag ttgtactaaa
    10081 attggtcaag tattgaaact attaaggtaa tcatggcaga acagatacgc actataacag
    10141 ataaagggca gttcccaaag atattttctc ttttttttgc caataatgat gtttttttaa
    10201 gaactgccag tggtgattta aaaatagtct atatgggttt tgctgatggc acggttgcat
    10261 ttaaaatccc ctatgttaaa aatatgcctg attcatgtgt tattttaacc cgtaaagata
    10321 gttatatcgt taatggtgtg tttaagttaa aagaaaaaga tgctgatgat atgtatgtct
    10381 tttatcctgt acagtttcag ataatttcaa gtccccgaaa ggaagaaaga attgagttag
    10441 gcacaggaaa atcagtattg tttattacta accttatttc tgattatata ttgcagaatt
    10501 cgctggcgct atcgctgaag aaagtagata atgttaagga aattttgcgg tttgatttag
    10561 aaaaacgttt cccctatgta aagatatatt ttttgaatga gggaatggct gatccccgaa
    10621 tgaaatattt ttatgcaaat ccagtaccgt attttattcc cagtatgaat gcacagcctg
    10681 atgctaaagg tcaaaagatg ttcaatacct atgtaaacga aatctattca aaagattaca
    10741 tgcttcaaaa cagaaagaat ttaatagccg agattgcagt gccggtatta tatcatggca
    10801 agatacccta tggctacata caggtcaata gcgataaacc attgactgat ggcatgtttt
    10861 cggtgataaa gcggttaggg gtggttgcag atgaactgtt taaaaagaac aagcttttta
    10921 tccctgaaac agaccgcctt cttattgctg atgcatcatt tagcggaatt ggcattgtgt
    10981 tcagggaacg aaagtatata cgatatttta aagaaggaag ctatgttatt ggggatattc
    11041 tgcttcccaa tggtgccagg gccaatgtgt tgtgtgtggt tcgcaatata acattaatgg
    11101 aaaataagat aattaaagtt ggttgccaga ttaaagacat agatgcattg agcgaagtcc
    11161 attaccacga gtatttagaa tccgctggcg taatagatta agtaacctgc caaataaaaa
    11221 tcttcatgaa ctaacaaaaa attccgatag gaaacagtag tatccttata tccaaggagg
    11281 gagttcatga atattaaaga cctgcaatct atcattttac aggagataga tatatatagc
    11341 acgttagtac agctggaaga acaaaagaaa gaagccatta cagccttcaa tggtgatttg
    11401 cttttttcat tatcaaaaga ccaggaaaca ttaatcctta atctggaaaa gctggaaaaa
    11461 tcacggcaac agttcataaa aacccatgta accggccatg cgtttcagcc acacgatatg
    11521 acgctcaagg actttattac gctaacccga atgcatgata acgaaaatct tatagtgcag
    11581 gctcaaaagc ttaaagaaac aattgagaaa tttgcacgcc ttcatgaagc caataagaag
    11641 ctgatacatg acaatataga gttttttaat gtaatcctga cttcattgcg gaaagcggta
    11701 acaattgaaa cagggtatgg gccagaagga ttggcaaaac gcagtgccag cgggtcagta
    11761 ttgatgaata aaacggtgtg aggtggtata tgaattcaac attcatggga atagaaattg
    11821 gtaaaaaagg gttacttgcc caccagcagg cattgcatgt taccggtcac aatatttcaa
    11881 atgctgcaaa tgaagaatat tcgcgccagc gtgtggttat aacagcatct gatccattgt
    11941 atgtgccggc attaaacagg gctaatcagc ctggcaatat tggacagggc agtgaagtgg
    12001 caatgattca gcgcatccgt gatgagttta ttgatgatcg catcatggtt gaaaagcagg
    12061 cctttgggta ctggaaagca cgcaatgatt ttatctacca gattgaaatg gtgtacaatg
    12121 aaccatcaga ccagtcaata cgcagtcggt tagatgcgtt gtgggaagga tttcaggaat
    12181 tgtcaaaata tccggaagag cgttcaaccc gtgaggtggt taaggaaaag gcgatagctc
    12241 tggcaaatga agtgaaatat acgtatggta aactgtatga acttcagaaa caggcaaaca
    12301 ggcagattgg gcatactgtg gaacaaataa acgaatatgc gcagactatc cgcgatttga
    12361 atgaacgaat actgaaagcc gaagcgttag gcgataatcc caatgacctc aaagacaagc
    12421 gtgatgcatt gatagaaaaa ctgtctcagc ttgtcaatat taatgtcagc cgcaccgata
    12481 aggatgagct tattgtgtat attggtgggg aaaacctggt acagggtgag gtgttcaggc
    12541 cacttgctgc cattgaggat ccggacaaca atggtatgta taaggtactg tggaaacaga
    12601 cactcactga tgttaatatt caaagcggtg agttagcagg ccttatttca atacgtgatg
    12661 gggttttgcg gcagaatata aatgacgtca atgcattcgc aataaatcta actgacctta
    12721 ttaatgaagt gcaccgtgat ggctttggga aaaacaatca gacaaataat aatttcttta
    12781 aacacattgc agtaagcgac aatgtggagg gaaactttga ccttaacaat gatggtatca
    12841 atgatgtaac agcactcttt aaaatttcag gcaacaataa ggttgatgct tccgctgcca
    12901 ttggcattac cggtacactt acctttgtga agaacaatgc gctggatcag gaaatcaaga
    12961 taaattatta tgctactgac acgctgcttg atgtcatcaa gcgtgtcaac gatgcaaaga
    13021 ttggcgttgt gggatacatc aatcataaca gccagttagc tttcaaagcc actatcgccg
    13081 aagataccga taagaaaaat tttattatac ggcacctgga agattcaggg caactactgg
    13141 tgggatatgc aggtattttg aaagagtcag gcccccaggg tgcatttgat tatcgcaggg
    13201 tggatgatat acgcaaaata attgcatcgc gtgaacatat caccatcacg cccatgttta
    13261 atcccgcatc gtatatggat attgatgatg caatcaaata cgatattgat tcaatagctg
    13321 ctgcaaaagg aaaagatttg ggtggtactg gcgactacaa cacatcaaat ggtgttggtg
    13381 atggatccaa tgcactcgca ctggcagcac tgaagcacaa gcatgctatg atagactcaa
    13441 atgcaacgtt caatgatttc tatacatcgc taatttcacg cattggttcg cagggtgagg
    13501 aagcaaaaga ccgaattgcc agccaggaaa cattgcttaa gaatctggcc aatcttcgtg
    13561 agtcggtatc gggaattaac ctggatgaag agatggccaa catggtacag ttccagcacg
    13621 gttataacgc ttcagcgcgg gtgattgcca tgattgaccg aatgcttgaa actatcatca
    13681 aattaggtca gggggtataa gccatgcaac gcgtaaccaa ccagatgatt aacaacacca
    13741 tgatatacaa cctgaataag catcagcagg aaatggataa aatgcaggat atgctggcta
    13801 cggggaaaaa tgtgcagttc ccgcgtgata atcctgtggc cgccaccaat cagatgctgt
    13861 ataagactgc actggttgaa atagagcagt atattaaaaa cattgacgag tcaaaatcaa
    13921 aattgagtga ggttgattcg gcactgcaat cggtcattcg gatttttcag cgtctgcgtg
    13981 ttctggcagt tcagggtgca catggcatct atacttcatt tgaacgaaag gaggccgcag
    14041 caaccgaaat aaatcagatg ttagaagagc ttgtcaacat agcaaatatc aaaggggcga
    14101 caggtaagcc catctttggc ggctatcaga caggaactga aaatatacct gatccatttg
    14161 tccccatcta tcagacgctt actgcaggca atcagggaaa tgccatgata ggcgtgcagt
    14221 accgtggcaa tattggcaaa atactgcgcg aggttgcaaa cagtgagtat ctggatgtta
    14281 atgttccggg aaactgggca ttctgggcaa ccaatcaggt gctatcggct aataaagacg
    14341 tgtcacaata ccgagcgctt gccacacagt cgtttaagat tgatggagtt tcaattcagg
    14401 ttgctgcagg tgataccatt gatatggtta tcgacaagat taataatgcc ggcctcagtg
    14461 taaaagcatc aataggtgcg cgcaacaatt tagttcttga aacaacggtt ccgcatgcta
    14521 tatggcttga ggatgaaggc agcggaacat ttttacgcga tgctgggatg ataaatcctg
    14581 atttccccaa tccgccaaat aatattgatc caacagtgtc ggtaagtggc atgtcaattt
    14641 ttgatatggt tatacaactg cgtgacgacc tggtacgtgg tgatcaggag cttgttggtg
    14701 ggcgggacct gggacttatt gatatggcgc tggataatgt gttacgccac attgctgcag
    14761 tgggtgcaaa ggaaaaccgt attgaagaac tggcaaaacg ttcagaatat gcaaagagta
    14821 atatacttga catactggca aaatctgaag ggattgattt cccagaaacc ataatgaact
    14881 tccgatggct tgaatcagta cacaactatg cgctggcagt tggtgcgcgg tcaataaagc
    14941 ccacactgat ggatttcctg cgataattac actatgaaga ggtgctaccg ttggtaacaa
    15001 ttgcaacaaa accatttggg caaattgaag ttgatgaacg gcagatcatt gatttccctg
    15061 aaggcattta cgggtttgag gacattaaaa aatttgtcat tctggatgcc aatgaaaaat
    15121 caccgtttaa atggctgcag gcctatgatg aacctgattt agcctttgtg attatacgtc
    15181 ccatagattt tatgattcag tatgaacttg aagttatgca ggaagattta gaggatatag
    15241 gtgcaaaaag ccctgacgaa gtaatagtgt ttgcgatagt taccatacca gaagaccctt
    15301 ccaggatgac agccaatctg caggggccag tccttattaa tcctaaaacc cgtaaaggca
    15361 aacaggccat atcactgagt gataaatata cggtgcgcca ttacatcctg gaagagataa
    15421 aaaaagcaca ggtgaagggg tgattgcatg ctggtactgg cgcgaaaagt taacgaaagc
    15481 atcatgattg gcgatgacat tgagatagtc attatagata ttaagggcga tcaggttaaa
    15541 ttaggaatca aggcaccaaa atctattgct attcaccgta aagagattta tgaggaaata
    15601 caaaaggaaa atattgctgc catgcaatcg cagttaaagc cggataccct gaaagatatt
    15661 accgatatat tgcagacaca taaagaaaaa aatattgaca attcaaaaaa ataactatca
    15721 ataatataca tctcccaacc aatatgatgg atatagagag gacccataat gcctgatatt
    15781 attgtgcaaa caaatgttgc atatcttgtg gctatatttt ttattatttt tataatccgt
    15841 gagatagttg cttttaaaaa gatattgaaa ctaaaatata tttttacacc tcttatcacc
    15901 tatattgtca ttattatagc tttatactta tcgtatgttc gtggttttac agtatataat
    15961 atctttatta ttgccgggct tgtatttgca ttaatcgccg atacttttct catgattgaa
    16021 gaggtcagct tttttatcca tggacttttt ttctttttat taacccacct ttgctatgtg
    16081 tatgcgttaa gtattaatta tcattttgga atcattgatg taattattat gctggttatt
    16141 ctggcaattt tctttctgta ttatctggta attcataagg caaaaggtac agtatatgtt
    16201 ccggtcatac tgtatatgct tgttttatca ctggtattga tgctggctgt tggtacatgt
    16261 attaaaaatg aatatcccag gggtgccatt gtaaccattg ctgcaatcct ttttgctata
    16321 tctgatggtg ttcttgcatt taaccagttt gtacacagaa tccctcatag tactgttgtt
    16381 acatggtcat tgtatgcacc tgcacagctt ctttttgcag tatcctgcta ttattgatga
    16441 aaagatattt aaacacccaa ttcttatttt ggtgatggaa gatcatcttg tatttatgtt
    16501 ctagaaaatt tttgttattt ttcttgtaaa gaagtactta tatttagtat aatttgttga
    16561 taatactttg tacatagtgt aaatcatata ctggtggtgt gttatgaaaa agatacttat
    16621 ttttgtactt atatcgttac tttcttgtgg gcaattaata cagcagagcc cacaaaagga
    16681 ttatgaactt atatacaaaa atttaaccaa tgccgaatca ctggaaaatc ctgtacaaaa
    16741 agataagctt atcaacaaag cctatactct tttgatgcaa cggcagcgtg aactgttaaa
    16801 agaaaaaaaa tctgatccac cagtagctgc attgcttgcc tactattatt ttttaaaagg
    16861 aaactatcag gatgcatcag cacaggttga acttgcacag tcttctgtat cagggaacaa
    16921 tccactgggg gtcattatac aggcacgtgt actgcttgct acaaaaggga aagcaggtgc
    16981 ccaaaacgca cttgatatcg taaagtcact gactgtaaca gaaagcccaa tggcatatat
    17041 tgtaacgggc gatgcttatt ttttaaaggg agattacgat aaggcacgtg ataattacac
    17101 aaaagcgtta ttgcttaaca gggaattaca ggttgttgct gccaaccggc ttgaagttat
    17161 atcccgcata aagacattgt ccatcaatgt ggcaaaagta tccgacttta ttcttgaacc
    17221 aacaatgaca agagaaaaac ttgcatacct gatgtatgaa atatttgaat tgcaaaagta
    17281 tattgctgct ggaaaaccct tcgatgccaa ttttattgat ctggaaaaat ctcagtataa
    17341 aaatgcaatt atatcgttgc gtggaaaagg tttcttttcg tatattcagg gtagtgtatt
    17401 tgaaccgttc atggtagtat cccgcggtga gatggcaaag attgttgagg attttttggt
    17461 attaacacga aataatgagg gattgcgatc aaaattcaaa aatgaatcac cttctttttt
    17521 caatgatata aaaaatgata atgagtatta taatgccatg cgattggctg ttgatatggg
    17581 tattatgagc gcctcattga gccaggatgc atatcctgat gaatcagtaa caggattgca
    17641 ggcaataatg ata
//
LOCUS       DAORYL010000065        17525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_2658_length_17525_cov_12.258157, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000065 DAORYL010000000
VERSION     DAORYL010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 17525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17525
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2658_length_17525_cov_12.258157"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1541)
                     /locus_tag="PLV81_11270"
     CDS             complement(105..1541)
                     /locus_tag="PLV81_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOM10718.1"
                     /translation="MSQTNFNEENFSELLEQSFIKSDNFEVGDTVTGKVILITGESIF
                     VDIQSKSEAVIDTQEFTDDKGNRTIKAGDTITAYIAAIKRGEIVLTSRIGKGVVSPDL
                     LHIAYKNQLPVHGTVKQTTNGGFIVSVGGIQAFCPLSHIDTKIQESDSYLNKSFDFAI
                     MRYEQQGKNIILSRRSLLDKIKEETIEKLKQQLHSGDTVQATIVQVLDAGLLVSLHSL
                     DAFVPKSELSHSRKPNMNQYEAGQTITAKILDLDWNKVKITLSIKQTLPEPWHYINHY
                     QVGNTYTGTIAKIIKNGAFVELDAGLEGFIPLSRMSYVKKLHDPHEVCNEGDSVQVKI
                     IDIDHSSKKITLELITSEENPWLTASESLRDSIQAATIEEITAKGLVVVLPNQVRAFV
                     PKEQCVIDRNADITKSYQVGSTIKVVVTTVNPDDRKCIASEKKAQEIEERQSYEKFIA
                     SSTTQGSSAFGQLFKQKYEEMQKKSKNT"
     gene            1794..2486
                     /locus_tag="PLV81_11275"
     CDS             1794..2486
                     /locus_tag="PLV81_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10719.1"
                     /translation="MRKLYLIIFIIASMIVTTGVFSQETKKDSLESEALVNEGKELDQ
                     QILTFCKRIEETIAKYKLMSIKDIRLLPFQISYSQGNDYIYLEKHFFVKDDLVPNKIK
                     IKKLKSIKIYTNGTTVSKLESIIREENYYENTVSEVKIIDPTPTSLGTDDIVFSYIRE
                     NKVILTEKRLSEVKNSTAFPIQNQLKREFYIPHLMYFYDVLLNIAETYYKGMKDVDST
                     MTEFLKDSTKYY"
     gene            complement(2528..3451)
                     /locus_tag="PLV81_11280"
     CDS             complement(2528..3451)
                     /locus_tag="PLV81_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOM10720.1"
                     /translation="MKFFHSAGFTGIQNLTIWGGWTDILIPRPEPGVIIEQIKKFKPT
                     FVPGVPTIFIGLLNNPEFRKMDLSFIKGFIAGAAPLPVETIKQLKELTGGDIINIYGL
                     TEISPMGTGSPWKGNIKPGTVGVPLPSTDIKIVDVESGTKEMGIGQSGEICFKGPQVM
                     KGYYKKPEETAVVLKDGWLYTGDIGTIDEDGYLTIVDRKKDMIISSGYNIFPLEIDEI
                     LFQHPKILEACTIGVPDPYRGEAPKAFVVVKPGEQLTKEEIIAYCKEKLAPYKVPKDI
                     EFIDALPKSAIGKILRKEVRELERKKREKTA"
     gene            complement(3447..3848)
                     /locus_tag="PLV81_11285"
     CDS             complement(3447..3848)
                     /locus_tag="PLV81_11285"
                     /inference="COORDINATES: protein motif:HMM:NF015166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561 domain-containing protein"
                     /protein_id="HOM10721.1"
                     /translation="MNIPLPIILHALFMFIGFVVLLYAASISMKKKTNQWLKKHRITA
                     LSGVTSVITGLIIMILFKESKGYPHLDSPHAIGGLVTCFMSLITPTLGYLITKGKQKV
                     KPLHKWSGRLTIILVAITIIFGFIRLLEIVN"
     gene            4034..5896
                     /locus_tag="PLV81_11290"
     CDS             4034..5896
                     /locus_tag="PLV81_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOM10722.1"
                     /translation="MGLIVDRRDQEFVLYEMLGLQELFATARFSDYSKDMFDMAIDLS
                     KRISEEEVLPRYMEGDREGAKLVNGEVKVPQCYHYLHKIMNDSGLFTMAVSPEAGGQG
                     FPYVIDLAAREYYVFNMGFLLYPEAAVGAAHLIEVYGTEEQKKKYMYKMYEGKWGGTM
                     VLTEPEAGSDVGNLKTKAIPQADGTYKIVGSKIFISGGDSDLFENIVHPVLARIEGDP
                     AGTSGISIFLVPKYLVNDDGTLGRKNDVTITGIEHKMGLKGSATCSMSFGDNGNCYAE
                     LLGQPRQGMKIMFQMMNEARLGMGLQGLGTASIAYLHALNYAKERKQGADLLNMQNPE
                     APRVSIVNHPDVRRMLLWMKSHVEGMRALVYLCALAIDRKEASEGEEAEKWHGIMELL
                     VPITKAYCTDMGFRVTELAIQIYGGYGYCQDYPVEQFMRDLKIASIYEGTNGIQALDL
                     VGRKMSQKKGANFINFLGEINKTLARYKDNPRLADISKDVQDAVNLLADMGMFFVQCG
                     KEGKFLIPISNAYPFLNLMGTVALGWLLFWQSGIAYEKLDEICKQNNVDVNDKKAVAQ
                     LAKDHKEAAFYSGKIHSARYYITHVLPHAQAYAKAIKSQNLSMLDIPEESFAIE"
     gene            6048..7490
                     /locus_tag="PLV81_11295"
     CDS             6048..7490
                     /locus_tag="PLV81_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HOM10723.1"
                     /translation="MAYKNLHNFITALEKAGELKRITAEVDPYLEITEIADRMSKTGG
                     PALLFEKVKGSLYPVLINAFGSYKRMQMALHCDSFDDIGQKIYNLITMQPPKTLTEKI
                     KALLMLKDVASLMPKQVSKAPCQKHVVTDGALLDALPILTCWPKDGGPFVTLPIVITK
                     DPETGIQNAGMYRMHKFSNTTTGMHWQYNKDGARHYRKYCQLQQRMPVAVALGGSPAI
                     TYAATAPLPPDIDEMLFAGFLDGEPVELVTAKTVDLLVPAEAEFIIEGYVDPGDETIE
                     GPFGDHTGFYSPADRYPVFHVTCITTRDNPVYPATVVGKPPMEDCYMAKATERIFLPF
                     LKMLIPEIVDIELPLEGVFHNCALVSIKKEYPGQAKKVINALWGLGQMASTKFIAVFD
                     ADIHLRDYSTVVWKLLNNVDPRRDLMIVEGPLDALDHSASFANFGGKMGIDATRKTKE
                     EGMGREWPEEITMSSNIIELVNKRWKEYGF"
     gene            7480..8325
                     /locus_tag="PLV81_11300"
     CDS             7480..8325
                     /locus_tag="PLV81_11300"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HOM10724.1"
                     /translation="MDFKKLSQLILIEQTVFALPFAYLGILFAGGGRLSTWIFASIAL
                     IAARTAGMCFNRLIDADIDAKNPRTQDRALPKGEVTREEVMRVAVLSCIIFVGSSYML
                     NMLCFYLSFVAIVLLISYSYFKRFSSASHLYLGFVEAAAPIGGYLATTGEFDLIPFIL
                     GFAIMTWIAGLDVVYAILDVDFDKQEGLYSIPSRYGVDKALIISALLYVLSFSSLITL
                     GVMTGRREAYWIGVFCVGIIFIYQQRLARSSDLQHAIKEFFKVNSFISPVLFLATLVD
                     VFFVM"
     gene            8927..10210
                     /locus_tag="PLV81_11305"
     CDS             8927..10210
                     /locus_tag="PLV81_11305"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOM10725.1"
                     /translation="MKKKITTIAILVIGLLLIIIVKPCDKGKPYNSYFANLNMELKKN
                     GPGRPVIIVDLDNLDKNLAIVKKQLQPSIKFRLTVKSIPCLWLIDYILTKMNSQHVMV
                     FHGPDLTYLANNSKNYDILLGKPMPINAVKEFYNSLKPGIQFKPEKQIQWLVDTPARL
                     QQYCDFAKQKNIRMKINIEIDVGLHRGGITTVEELDQMLNTIEKNSKILQFSGFMGYD
                     VHSASALAIFTSKKKAIQNAFNDTMTRYAMFYNYGKKKHPALFDGNLTFNSGGSQTYM
                     LFNGTLPVNDIAMGSALLKPSDFDKPLLSEHVEALFIATPVLKRLEGTTIPFIEVISN
                     LMAKWNPNRQVTYFMYGGGWRAHYYSPEGLIDNTIYGFSTNQAIVNGSRATALQVDDN
                     IFLLPTQSEAIMREFGDIIMIRKGRIVGKYPAIPQ"
     gene            10264..10995
                     /locus_tag="PLV81_11310"
     CDS             10264..10995
                     /locus_tag="PLV81_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10726.1"
                     /translation="MTSKSNKTTKYFVGFQIALVVVFTTIDFIGLLLGYKINFVAIPK
                     GLLSLSATLFVWFGLQDTIAQKDKRFLRLIFTCTFITDMCVLTWTHFLPDMYTLLTIG
                     GIVALPMLLTVIIRHTEMFKAVQKKDIGILIVLYAVAFIIIIYLFEMLQRYNILGISI
                     AYALLLVPVAWSGWLARRSEYFPAHSRWMIPAATTSYFIMDVIGIFYNVRYGSNPDLF
                     YFFTWPLYAVFLILMPLSGKNKLLT"
     gene            11864..12466
                     /locus_tag="PLV81_11315"
     CDS             11864..12466
                     /locus_tag="PLV81_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013180507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HOM10727.1"
                     /translation="MIIVCITGASGAILGIRLIEELLQCNKEVTCIVSQSAWQVMHYE
                     LQTDAKTIAAILKNRDVQNNLLENLKEYSPDDFFAPVASGSFPFDAAVVIPASMKTVA
                     AIAHGYADNLIHRVCDIALKEKRTLIVVPRETPMSTIHLHNLYTIALNGTSVVMPLPA
                     FYNFPKTIDDVINFVVGRVLDLLHIEHTLYKRWNNENNNR"
     gene            12447..13529
                     /locus_tag="PLV81_11320"
     CDS             12447..13529
                     /locus_tag="PLV81_11320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HOM10728.1"
                     /translation="MKIITDSNLEAIAEKVEAGIPITDDEALAMLLTSDIMGICSIAH
                     SIKQKLHGNVVYYGVNMNLNYTNVCMLRCPLCAFSRDKNDADAYVLSIDDIANKITQA
                     GDIDEVHIVGGLHPDLPFSYYEDMLKTIKTIKPTIHIVAFTAVEYDYLSKKFDIPLDE
                     LFVRFKNAGLGSIPGGGAEIFAPEVREIIAPKKISGKRWLEVMAIAHRNGIKTNATML
                     CNHIEKPADIVDHLRHIRQLQDTTKGFKTFVPLAFHEEHTKIKAKRKEPTGYDIIRMY
                     ATARIYLHNVPHIKALWMYVGPKVAQVLLQCGVDDIGATYYDEKVVHSAGAKTPAWGS
                     EPFLQHLIRDAGFTPLRVNASYIPTR"
     gene            13533..14339
                     /locus_tag="PLV81_11325"
     CDS             13533..14339
                     /locus_tag="PLV81_11325"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HOM10729.1"
                     /translation="MKLGYINYLNCFPFYYHMFHVKPLPGVEVVPAYPSQLNTMLKNG
                     VLHMSPISSAAYAEAQQDLQLLPEFCLSSIGYVRSVILVSSKPIEMLDHCVVGLSQAS
                     QTSVMLLKIILQKFYGLTPHYISSDPYPVLHDVDAKLVIGNEAMMHETEPLPYIYDLG
                     DLWLQKTGYPVVFAVFAVRNDAIDYAETINAVCASYRMSLNELAKNPHDVIEKARQRY
                     PDIAYDIAHYYTLLKFEFTDELKKALLYYYSLASELQLLPQVPSLQLYGR"
     gene            14349..15419
                     /locus_tag="PLV81_11330"
     CDS             14349..15419
                     /locus_tag="PLV81_11330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HOM10730.1"
                     /translation="MKSIDNIYHPVADRRRIDPHDAVSLYQYDIATLGVLANMRREQI
                     VPGNEVGFVIDRIINYSNRCVAMCNFCAYHARAGKIPPYDLTMDDILQKINELVAIGG
                     TQVMLQGGLHPEYTLQHYIDMVKTIKKHYPTIYLHSFSPSEVVHIAKQSNCSIDDVIG
                     SLQTAGLDSIPGASDLLVDEVRMRVSPRKITVQQWCDVVRSIKKHGMHTTATMTYGMG
                     ETLEQRIEHLEVVRRMQDETGIIMGFIPWSFSPKGTYMDDIIPATGLDYLKTVAIARI
                     FLDNVPYLHAGWLTEGMALAQIALAMGANDMGGILMEEKVVKATGVTTTTNIEQICDI
                     IINAGKVPLQRDSKYRPIRRYV"
     gene            15416..16192
                     /gene="ubiE"
                     /locus_tag="PLV81_11335"
     CDS             15416..16192
                     /gene="ubiE"
                     /locus_tag="PLV81_11335"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOM10731.1"
                     /translation="MRYRVDNDTPHERKAKIRSMFDSIVDGYDRANRILSLGIDRSWR
                     KTVVEHCECKPSDVVLDICCGTGDLSALIRGTGAHVVSLDFSENMIQKGREKGNIDDL
                     ATVADASKLPFKDNIFTKVCISFGIRNIPDIDNFLEEVYRVLRPGGTFIILELTRPRY
                     NIFRLLYFFYLYTILPVLGGIIAGSFNAYRYLSKTIHTFIDPVTLTAMQEKHGFIDIT
                     YKSMTLSIATMYISKKPPVGTSFLSIPLSKQSHPNNVIIH"
     gene            complement(16189..17088)
                     /locus_tag="PLV81_11340"
     CDS             complement(16189..17088)
                     /locus_tag="PLV81_11340"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOM10732.1"
                     /translation="MVAWLNGSLTDIHNAKVSLLSHSFSRGTAIFEVLEIVTNGKGTA
                     LLGLHQHCERFFNSARALHIQLPYNKEQIIEAIVTCAKANNCKNGLVKFFAYFDDIEL
                     TIVPHSMNVSLAIFCINYETLNFDVHELSKPSRACLSTYKKIDPLTVPVYAKVAGNYV
                     NSYLAQMEAKQKGYSDAILVDTQGNIAEAATANIFFVKGQSLYTPTLRSIMPGITRMI
                     VLDIAHHDNIPATECDIPAESINQFSEAFFSGSVNNIQPIESIDDIIFRPVPGTISQI
                     IQDKVTSLISGNHPLTNKYLYYF"
BASE COUNT         5360 a         3150 c         3679 g         5336 t
ORIGIN      
        1 ttttcaatct tcttgtggat ttcgttaata tattggatat tgttttctac gtatgttcta
       61 tatgctaaaa gttcattgtg tgaagtgttg ctatccatac tggtctatgt gtttttagat
      121 tttttctgca tttcctcgta cttctgttta aacagttgtc caaaagctga agaaccctgc
      181 gtggtactgg aagcaatgaa tttttcgtac gactggcgct cttcaatttc ctgcgctttt
      241 ttttctgaag caatgcattt tctgtcatcg ggattaactg tagtcactac aaccttgatg
      301 gttgacccta cctgatatga ctttgttata tcggcattac ggtcaattac gcattgctct
      361 ttaggaacaa atgcacgcac ctggtttggc agcacaacaa caagcccttt agcagtgatc
      421 tcttcaatgg ttgcagcttg tatagaatcg cgcagtgatt cacttgcggt aagccaggga
      481 ttttcttcac tggtaataag ttccagtgta atttttttac tgctgtgatc aatatcaata
      541 atttttacct ggacactatc gccctcatta catacttcat gtgggtcatg taacttttta
      601 acataactca tgcgtgaaag tggtataaac ccttcaaggc cggcatcaag ttcgacaaat
      661 gcaccatttt ttattatttt ggcgatagtt cctgtataag tattcccaac ctggtagtgg
      721 ttgatgtaat gccagggttc aggcaatgtt tgcttaatac ttaatgttat tttgacttta
      781 ttccagtcta aatcaagtat ctttgcagtg atggtttgac ctgcttcata ctgattcatg
      841 tttggtttgc ggctgtgcga taactcactt tttggcacaa aggcgtcaag tgaatgtagc
      901 gatactaaaa ggccagcatc aagtacctga actatagtag cctgaactgt gtcgccacta
      961 tgtaattgct gtttcagctt ttctatggtt tcttctttga ttttatcaag caatgacctt
     1021 cgcgacagaa tgatgttttt accctgctgc tcatagcgca taatggcaaa gtcaaagctt
     1081 ttatttaggt aactatcgct ttcctgaatc tttgtatcaa tatgtgatag tggacaaaat
     1141 gcttgaatac caccaacgga aacaataaag ccaccattgg ttgtttgttt gacagttcca
     1201 tgtacaggta attggttttt atacgctata tgcaaaagat caggtgaaac cacacctttg
     1261 ccaattcgtg atgttaatac tatttcaccg cgtttgatag cggcaatata cgcagtaata
     1321 gtatcaccag cttttatggt acggttgcct ttgtcatcag tgaattcctg tgtgtcaatg
     1381 acagcctcac ttttggattg aatatcaaca aaaatgctct ccccggtgat gagaatgact
     1441 ttacctgtaa cggtatcgcc aacttcaaaa ttatcggatt tgatgaacga ttgttccagt
     1501 aattcagaaa agttttcttc attgaaattt gtttgtgaca tgaatgtaac ctgttgcatt
     1561 agtaaatatc agtgatgcaa tgtacaatat ttaatgaagc aaaaattgtc aatattgttt
     1621 tttgtggtca acctgccatt tattataata tattatttta ttaacaaaaa tattgctaca
     1681 tatcgataat tagataagca aggcctggtt taacaaattt ttctttaaat ttttataata
     1741 tgatatacaa tacgtagtaa atttccttaa ttatatataa tcggaggata tgaatgcgta
     1801 aactgtattt gattatattt attattgcat ccatgatagt tactactggt gttttttcac
     1861 aggaaactaa gaaagattca ctggaatctg aagcacttgt taatgaaggt aaagaattag
     1921 accagcaaat acttacattt tgtaaaagaa ttgaagaaac aattgccaaa tacaaattga
     1981 tgtcaataaa agatatacga ttattgccat tccagatatc atactcacag ggtaatgact
     2041 acatttatct ggaaaaacat ttttttgtaa aggatgatct ggttccaaac aagataaaga
     2101 taaagaaatt aaaatctatc aaaatttata ccaatggaac aactgtgagc aagctggaat
     2161 caattatacg ggaagagaat tattatgaaa acaccgtttc agaagtaaaa ataattgacc
     2221 caacacctac ttcattggga accgatgata ttgtgttttc atatatacgt gaaaataagg
     2281 taattctaac agaaaaacga ttatctgaag ttaaaaattc aacagcattc cccattcaga
     2341 accagttaaa gcgagaattt tatatacccc atttaatgta tttttatgat gtattattaa
     2401 acatagctga aacatattat aagggtatga aggatgttga cagtaccatg acggaatttt
     2461 tgaaagattc aacaaaatat tattaataca aacaagggcg ggattcaaac ccgcccttgt
     2521 tactttatta tgctgtcttc tcacgttttt tcctttctag ttctctcact tcttttcgta
     2581 aaatcttccc tattgcactt ttaggcaatg cgtcaataaa ttctatatct ttaggaactt
     2641 tataaggtgc aagcttttcc ttgcaataag caataatttc ctcttttgta agttgctcac
     2701 caggctttac cacaacaaat gcttttggtg cctctcctct atatggatct ggcacaccaa
     2761 tagtacatgc ttcaagaatc ttggggtgct ggaataatat ctcatcaatc tccagtggga
     2821 aaatattgta tccgcttgaa ataatcatat cttttttgcg atcaactatt gtaaggtagc
     2881 catcttcatc aatagttcca atgtcgccgg tatacaacca tccatctttt aaaacaacag
     2941 ctgtctcctc aggtttttta taatatcctt tcattacctg agggccttta aagcatattt
     3001 cacccgactg accaatcccc atttcttttg tgccagattc aacatcaact attttaatgt
     3061 ctgtactggg taatggtaca ccaacagtgc caggttttat gttccctttc catggtgaac
     3121 ctgtacccat tggcgaaatt tcagtaaggc catatatgtt gatgatatca ccaccggtta
     3181 attctttcag ttgctttatc gtctcaactg gcagtggtgc agcaccggca atgaagcctt
     3241 taataaagct taagtccatc tttctgaatt cagggttgtt cagtaaccca ataaatattg
     3301 ttggtacccc cggaacaaat gttggtttga attttttaat ctgttcaatt attactcctg
     3361 gttctggtct aggaataagg atatctgtcc agccacccca gatagtaaga ttctgaattc
     3421 ctgtaaaacc tgctgaatga aaaaatttaa ttaactatct ccaataaccg tataaaacca
     3481 aaaatgatgg ttattgcaac aagaattata gttaacctgc cagaccattt gtgaagaggt
     3541 tttactttct gtttgccttt agttatcaaa taacccagag tgggtgtaat cagtgacatg
     3601 aaacaggtaa ccagaccacc aattgcatga ggggaatcca ggtgtggata ccctttagat
     3661 tctttaaaca gtatcataat aatcaatcca gttatgactg atgtcactcc agaaagtgca
     3721 gttatacggt gtttttttaa ccattgattg gttttctttt tcattgaaat actggcagca
     3781 tagagcagca caacaaatcc aataaacata aataatgcat gaagaataat tggtaatggt
     3841 atattcatgg ctaacctcta ctttaaaaaa agttaagaaa taatactggc ataataatat
     3901 attgtggctg atagaaaggt caataaaata aaattggaac taccagtaaa ctacaataaa
     3961 aaatttgtga agagcaaatt atatactata attatatata taaattttaa taaatattat
     4021 tatgaggttg tttatgggtt tgatagttga caggcgagat caggaatttg tgttgtatga
     4081 gatgctggga ttgcaggaat tatttgcaac agcacgtttt tcagactact caaaagatat
     4141 gtttgatatg gcaattgatc tttcaaagcg tatttctgaa gaagaagttt tgccaaggta
     4201 tatggaaggc gacagagaag gagcaaaact ggtgaatggc gaagttaaag ttccccagtg
     4261 ctatcactat cttcataaaa taatgaatga cagtggccta tttaccatgg cagtatcccc
     4321 cgaagcaggt ggtcagggat ttccgtatgt gattgatctg gctgcacgtg aatactacgt
     4381 attcaatatg gggtttttat tatatcctga agccgcagtt ggtgctgcac acctcattga
     4441 agtgtatggt actgaagagc aaaagaagaa atacatgtat aagatgtatg aagggaaatg
     4501 gggtggcaca atggtattga ctgaacccga agcaggttcg gatgttggaa atttaaagac
     4561 aaaagctatt cctcaggcag atgggacata taaaattgtg ggaagcaaaa tatttatttc
     4621 cggtggcgat tcagaccttt ttgaaaatat tgtacatcct gttcttgcac gaattgaagg
     4681 agacccggca ggtaccagtg gtatttccat atttcttgtt cccaaatatc tggtaaacga
     4741 tgatggtact ctgggaagaa aaaatgatgt aactatcacc ggcatagaac acaagatggg
     4801 attgaaaggt agtgctacct gttcaatgag ttttggtgat aatggcaatt gttatgctga
     4861 actattgggt caacctcgcc agggtatgaa gataatgttc cagatgatga atgaagctcg
     4921 tctgggtatg gggctgcagg ggcttggtac cgcttcaatt gcatacctcc atgctttaaa
     4981 ctatgcaaaa gagcgaaagc agggtgcaga tttattgaat atgcaaaatc cagaagcacc
     5041 acgtgtttca attgtcaatc atccggatgt tcgtaggatg cttttatgga tgaaatcaca
     5101 tgttgaaggc atgcgtgctc tggtatattt gtgcgctctt gctattgacc gcaaagaagc
     5161 aagtgaaggc gaagaagcag aaaaatggca tggaattatg gaattgctgg ttcccataac
     5221 caaggcatat tgtacagata tgggcttcag ggtaacagag cttgctattc aaatttatgg
     5281 cggttatggg tattgtcagg actatcctgt tgaacagttc atgcgcgatt tgaaaattgc
     5341 gtccatatat gaaggcacca atggcataca ggcacttgat ttagttgggc gaaagatgtc
     5401 gcagaagaaa ggggcaaact tcattaattt ccttggtgag attaacaaaa ctcttgcacg
     5461 ctacaaggat aatccacggc ttgctgatat ttcaaaggat gttcaggatg cagtcaattt
     5521 acttgctgac atgggcatgt tctttgtcca gtgtggcaaa gaaggtaagt tcttaattcc
     5581 aatcagcaat gcatatccat tcttaaatct tatgggaact gttgcattgg gctggcttct
     5641 tttctggcaa agtggcattg cgtatgaaaa gcttgacgaa atttgcaaac agaataatgt
     5701 tgatgtaaat gataaaaagg ctgttgcgca actggcaaaa gatcataaag aagcagcatt
     5761 ttattctggt aagattcaca gtgcgcgcta ctatattaca catgtactgc cgcatgcgca
     5821 ggcatatgca aaagcaataa agagtcagaa tctttcaatg cttgacattc cggaagaaag
     5881 ctttgcgata gagtaagcag gttgtcacgc gtcacaatca acactaaagc gggctactaa
     5941 atggcccgct ttttttatag taactatcgc acatataaaa aatacttgcg ttaacgttat
     6001 ttttatggcc gatagttacc aactataaat gtggaaatga ggtttacgtg gcatataaaa
     6061 atttacacaa ctttattact gctttagaaa aagctggtga gctgaaacgt atcacagcag
     6121 aagtagatcc atatttagag attacggaaa tagctgacag aatgagtaaa accggtggtc
     6181 cagcacttct ttttgaaaag gtaaagggtt ctttataccc ggtactcatc aatgcgtttg
     6241 gaagctacaa acgtatgcag atggcactgc actgcgattc ttttgatgac attggtcaaa
     6301 aaatttataa tctcattacc atgcagcctc ctaaaacatt aaccgaaaaa ataaaggcat
     6361 tactgatgct aaaagatgtt gcatcactga tgcccaaaca ggtgagtaaa gcaccatgcc
     6421 agaagcatgt ggtaaccgat ggcgctttac ttgatgctct gccaatttta acatgctggc
     6481 caaaggatgg tggtccgttt gttacattac ccattgtgat tacaaaggat cctgaaacag
     6541 ggattcagaa tgcaggtatg tatcgcatgc acaagttttc taacacaaca accggtatgc
     6601 actggcagta taacaaagac ggtgctcgcc actatagaaa atattgtcag ttgcagcaaa
     6661 ggatgccggt tgctgttgct cttggcggaa gcccggccat tacatacgct gcaacggcac
     6721 cattgccacc tgatattgat gaaatgctat ttgctggttt tttggatggt gaaccagttg
     6781 aacttgttac agcaaagaca gtggatttac tggtacctgc tgaagccgag tttattattg
     6841 agggctatgt tgatcctggt gatgagacca ttgaaggtcc ctttggtgat cacacaggat
     6901 tttattctcc tgctgacaga tatccagtat ttcatgttac ctgtataaca acgcgcgata
     6961 atcctgtgta tcctgcaaca gtagtgggta aacccccaat ggaagactgc tatatggcaa
     7021 aagccactga gcgtatattc ctgccttttt taaagatgct gatacctgaa attgttgata
     7081 ttgaacttcc gcttgaaggt gtgtttcaca actgtgcgct tgtatcaatt aaaaaagaat
     7141 atcccgggca ggcaaagaaa gttatcaatg ccctctgggg gttggggcaa atggcatcga
     7201 ctaagtttat tgcagtgttt gatgcagaca tacatcttcg tgattacagc acggtggtat
     7261 ggaagctatt aaacaatgtt gatccgcggc gtgacctcat gattgtggaa ggaccactgg
     7321 atgcgcttga tcattcagct agctttgcca attttggtgg gaagatggga attgatgcaa
     7381 cacgtaaaac aaaggaagaa ggtatgggca gagagtggcc tgaggaaata acaatgtcat
     7441 cgaatattat tgaactggta aacaaaaggt ggaaagagta tggattttaa gaagctttcg
     7501 caacttattt tgattgaaca aacggtgttt gcgctgccgt ttgcatatct gggtattctt
     7561 tttgcaggcg gtggcaggct ttcaacctgg atatttgctt caattgcgct gattgctgcg
     7621 cgaactgccg gcatgtgctt taacaggctt attgatgcag acattgatgc aaaaaatccc
     7681 cgtactcaag accgggcatt gcccaaaggt gaggtcactc gtgaagaagt gatgcgtgtt
     7741 gcagttcttt cttgtatcat ttttgtgggg tcatcataca tgcttaatat gctgtgtttt
     7801 tatctttcat ttgtggcgat agttctttta ataagctatt cgtactttaa aaggttttca
     7861 tcagcctcgc atctgtattt aggatttgtg gaagcagctg cacccattgg tggctattta
     7921 gcaacaacag gtgaatttga tctgataccg tttatattag gctttgccat tatgacgtgg
     7981 attgctggcc ttgatgtggt gtatgccatc cttgatgtag actttgacaa acaggaaggc
     8041 ctatactcaa taccttcacg gtatggagtg gataaagcgt taataatttc agcgttatta
     8101 tatgtgttat cgtttagttc gcttattact cttggtgtta tgacaggccg tagagaagca
     8161 tactggatag gtgtattctg cgttggtatt atttttattt atcagcagag gttagctcgc
     8221 agcagtgatt tgcagcatgc aataaaggaa ttttttaagg ttaattcatt catatcacca
     8281 gtgctgtttt tagcaacact ggttgatgtg ttttttgtta tgtgaggtta cctgcctttt
     8341 actatttatt tagcattttt acgcaggcgc tcaagcacct gcataatgag aaatacatgg
     8401 gctcagatct ttgtaatcac aatttatgtt ccttagcgca ctatgtgtat agtaaagaat
     8461 ggtatatact taggttgcga aagatcgctg aataaagatg tcaaagctgt ttttgtacta
     8521 tgtatcagta catagctata aattatttac acagagaatg aaagagtgcc gtatttttgc
     8581 cgagcaccgc aggacacggc gggaaaaggg cgaggactgt ttgagtcccg agaccgagga
     8641 agtcggtatc gggacgagtt ccgcagcccc cgccgtggac gaggagcgca gggaatccct
     8701 catgaaatga gggacaaaaa acgataggcg ccaagccggg gtaaagggga tgacaagggg
     8761 aaaaggggct ggcgtatgaa gccctttttc cccttgtgaa aagaatgata tggagttgtt
     8821 gccgtctgga caaaagaaag tgcagaaaag caaaaccaat tttttaattt gacttacatc
     8881 cccatacagg tatatcatat atataaaaaa aaggaggaac acaatcatga aaaagaaaat
     8941 aacaactatc gccatacttg tcattggatt gctattaatt attattgtta aaccctgtga
     9001 taagggcaaa ccatataatt catattttgc taatttgaat atggaactaa agaaaaatgg
     9061 tcctggcaga ccagttatta ttgttgatct tgataatctt gataagaact tggcaatagt
     9121 taaaaaacaa ttacaacctt ctataaaatt tagattaaca gtaaagtcaa ttccatgtct
     9181 gtggcttatt gattatattt taacgaaaat gaattctcaa catgtcatgg tttttcacgg
     9241 accagattta acctaccttg ccaataattc aaaaaattat gatatacttc tgggaaagcc
     9301 aatgcctatt aatgccgtta aggaatttta caattcatta aaaccaggaa tacaatttaa
     9361 accagaaaaa caaatccagt ggctggtgga cacacctgca aggctgcagc aatattgtga
     9421 ttttgcaaaa caaaagaata ttaggatgaa aataaatatt gaaattgatg tgggattaca
     9481 tagaggcgga attacaactg ttgaggaact cgatcagatg ctgaatacaa tagagaaaaa
     9541 ttccaaaatt ttacaatttt ctggctttat gggttatgat gttcattctg catctgctct
     9601 tgcaatattt acctcaaaga aaaaagccat acagaatgca tttaacgata ctatgacacg
     9661 atatgcaatg ttttacaatt atggtaaaaa gaaacatcct gcactttttg atggcaattt
     9721 aacatttaac agtggtggca gtcaaaccta catgttattt aatggtacat tacctgtcaa
     9781 cgatatagca atgggttcgg cattgcttaa accatccgat tttgataagc cacttttatc
     9841 ggaacatgtt gaagcattat ttattgcaac accagtttta aaaagactgg aaggtacaac
     9901 gattcctttc attgaagtaa tatcaaatct aatggccaaa tggaatccca acagacaggt
     9961 gacgtatttt atgtatggtg gtggttggcg tgctcattat tattcacctg aagggctcat
    10021 tgataatacc atctatggat tcagtaccaa tcaggcaatc gtaaatggtt cgcgggctac
    10081 agcacttcag gttgatgata atatattttt actgccaacg cagagtgaag caatcatgcg
    10141 tgagtttggc gatataatca tgatacgtaa gggcaggata gtaggaaaat atcctgcaat
    10201 accacaataa gaaaaaaatg ggggtcagag ccttgcattc cattttctga aaggagagtt
    10261 gccatgactt caaagtccaa taagacaaca aaatattttg ttggatttca gatagcactg
    10321 gttgtcgttt ttaccaccat tgatttcatt gggttattat tggggtacaa aatcaatttt
    10381 gtggcaatac ccaaagggtt gctttcatta tcagcaacat tatttgtgtg gtttggattg
    10441 caggatacta tcgcccaaaa agataaacga tttttgcgtt taatctttac ctgtacgttt
    10501 attactgata tgtgtgtcct gacgtggact cattttttgc ctgatatgta cacactcctt
    10561 actattgggg gtattgtggc tttgccaatg ctattaacag tgattatacg ccatactgag
    10621 atgttcaaag cggtacaaaa gaaagatatt ggtatcctga tagtactata tgcggtagca
    10681 tttattatca ttatatatct ttttgaaatg ttacagcgct ataatatttt gggcatatca
    10741 attgcatatg cactgttgct ggtgcctgtt gcgtggtcgg gttggctggc gcgtcgctct
    10801 gagtatttcc cagcgcattc gcggtggatg atacctgctg caacaacaag ctattttatt
    10861 atggatgtca ttggcatatt ttataatgtt cggtatgggt ctaatcctga tttattttat
    10921 ttttttacct ggccattgta tgcagtgttt ttgatattaa tgcctctatc aggcaaaaat
    10981 aagctgttaa cctgatttta tataacaaca accgaagctt ttttattaaa tggatttctg
    11041 cgtaatgcca gtgaaaacag atacgggtaa tcattttgaa gatgattcat aataaagata
    11101 tgtaccagtg tttgtgtttc ataagttctt tatttggatt gaataatttg atgtcaagat
    11161 gttttttgat gagcgtcatg ttctggtatt ttctaagtgc cctctatgtc aggaaaagga
    11221 attattgatc acacagagaa cgcatgtgtc ggagtctaac cataaaataa taatgtatac
    11281 atggttgagt tactataaac agcgaagaca ggatgtcgat gctcttagta ttacgtgtta
    11341 gtacatggct ataaaatatc ttatactgag aataaaaaag tgccgttttt tttgccgagc
    11401 accgcaggac acggcgggaa aagggcgagg actgttcgaa gcaccgatgc aatcggtgca
    11461 agttccgcag cccccgccgt ggacgaggag cgcagggaat ccctcatgaa atgagggaca
    11521 aaaaacgata ggcgccaagc cggggtaaag gggatgacaa ggggaaaagg ggctggcgta
    11581 tgaagccctt tttccccttg tgaaaagaat gatatggagt tgttgccgta ttgacaaaag
    11641 aaagggcaga aatgttaaga attaaaatct aagcgtacac tgcgtaaagt caaggaatga
    11701 tgcatcacgc agagaattca tgaaacgctg tgtacgcagg ggtaaatgag gtctggtata
    11761 catcaattat cacaggtggt taagtgctta agcgcctgca tactatggta agatggcaaa
    11821 attactttag aaggaattaa cgcattgtga tacaattaat cccatgataa ttgtatgtat
    11881 aacaggtgca agtggtgcta ttttaggaat acgccttata gaagaattgt tacaatgcaa
    11941 taaagaagta acctgcattg tttcacaaag tgcatggcag gttatgcact atgagttaca
    12001 gacagatgca aaaacaatag cagctatttt aaaaaaccga gatgtacaaa acaatctgct
    12061 agaaaacctg aaagaatatt cacctgatga tttttttgcg cctgtggcaa gcggtagctt
    12121 tccctttgat gctgcagtgg ttattcctgc atcaatgaag accgtggccg ctatcgcaca
    12181 tgggtatgcc gataatctta tccaccgtgt gtgcgatatt gcactgaaag aaaagcgtac
    12241 gttaattgtt gtgccccggg aaacaccaat gagtacaata cacctgcata atttgtacac
    12301 gatagcactc aatggaacga gtgtggttat gcccttacca gcattttata atttccccaa
    12361 aactattgac gatgttatta attttgttgt tggcagggta ctggacctgt tacatattga
    12421 acatactctg tacaaaagat ggaacaatga aaataataac cgatagcaac cttgaagcaa
    12481 ttgcagaaaa agtggaagct ggtattccca tcactgacga tgaagccctt gccatgcttt
    12541 taaccagtga catcatggga atttgttcca ttgctcacag cataaagcaa aagctacatg
    12601 gcaatgtagt ctattatggc gtaaatatga atcttaacta taccaatgta tgcatgctgc
    12661 gatgtccgtt gtgtgcattt tcccgcgata aaaacgatgc cgatgcttat gtactgtcaa
    12721 ttgatgatat agctaacaag atcacacagg ctggtgatat tgatgaagtg cacatcgttg
    12781 gtgggcttca tcctgattta ccattttcat actatgaaga catgctcaaa accattaaaa
    12841 caataaaacc cactattcac atcgttgcct tcactgctgt ggaatacgat tatctttcaa
    12901 agaagtttga tataccgtta gatgaactat ttgtgcgctt caaaaatgca gggcttggat
    12961 caattcctgg tgggggtgct gaaatctttg cacctgaggt gagggaaatt atagccccaa
    13021 aaaaaatcag tggtaaacgc tggttagagg ttatggctat agctcacagg aatggcatca
    13081 aaaccaatgc aaccatgctt tgtaaccata ttgaaaaacc tgctgacatt gttgatcatt
    13141 tgcgtcatat acgccaattg caggatacaa caaaaggttt caaaacattt gtgccgctgg
    13201 catttcatga agaacacaca aaaattaaag caaagcgaaa agagccaaca gggtacgata
    13261 ttatcaggat gtatgcaaca gcacgcatct atttgcacaa cgttccgcat ataaaggcgc
    13321 tatggatgta tgtgggacca aaggttgctc aggttcttct gcaatgcggt gtagatgata
    13381 ttggcgcaac ctactacgat gaaaaggtgg tgcacagtgc aggggcaaaa acgcctgcat
    13441 gggggtcaga gccttttttg cagcacctga tacgcgatgc gggttttacg ccactgcgtg
    13501 tcaatgcaag ctatatacca acacggtgaa ttatgaaatt aggctatata aattatctca
    13561 attgctttcc attttactat catatgtttc atgtaaagcc attacctggt gttgaggtgg
    13621 tgcctgcata tccaagccag cttaacacaa tgctgaaaaa cggcgtgctg cacatgagcc
    13681 ccatatcatc ggcagcgtat gctgaagcgc agcaggattt gcagcttttg cctgagtttt
    13741 gtttaagttc cattgggtat gtacgttcgg tcattcttgt aagcagtaag cccattgaaa
    13801 tgcttgacca ctgtgttgtt gggctgtcac aggcttcaca aacttcggta atgctgttaa
    13861 aaataatctt gcagaaattt tacggattga cgccacacta cataagctct gacccctatc
    13921 cagtgctgca tgatgttgat gcaaagcttg tcattggtaa tgaagcaatg atgcatgaaa
    13981 cagagccact gccgtatatc tatgacctgg gtgatctgtg gcttcagaaa actggttacc
    14041 ctgtggtctt tgccgtgttt gcagtgcgca acgatgcaat agactatgct gaaaccatta
    14101 acgcggtgtg tgcgtcatac cgcatgtcat tgaatgagct tgcaaaaaat ccccacgatg
    14161 ttattgaaaa ggcacggcag cgttaccctg acatagccta tgatattgct cattactaca
    14221 cattgcttaa gtttgaattt acagatgagc ttaaaaaggc tttgctgtat tattattcac
    14281 tggcttcgga gcttcaattg ttgccacagg ttccatcatt acaattatat gggcgatagt
    14341 tactggatat gaaatcaatt gacaacatat accatcccgt agctgaccgc agacggatag
    14401 atccgcatga cgccgtttcc ctgtatcaat acgatatagc tacgttaggt gttttagcaa
    14461 acatgcgtcg cgagcagata gttcctggaa atgaagttgg ctttgtcatt gaccgcatca
    14521 taaattattc caacaggtgt gtggcaatgt gcaatttttg cgcatatcat gcccgtgcag
    14581 ggaaaatacc gccgtatgat ttaaccatgg atgacatttt acaaaagatt aatgagcttg
    14641 ttgccattgg tggcacacag gtcatgttgc agggtggatt gcatcctgaa tatacgttac
    14701 agcactatat tgacatggta aaaacaataa aaaagcatta tcccactata tacctgcatt
    14761 cattttcccc atccgaagtg gttcatatag caaagcaaag taattgcagc attgatgatg
    14821 ttattggcag tctacaaact gcaggtcttg attcaatacc aggtgcttct gacttgcttg
    14881 tggatgaagt acgcatgaga gtaagcccgc gtaaaattac agtacaacag tggtgtgatg
    14941 tagttcgttc aataaagaaa catggcatgc acaccacagc aaccatgacc tacggcatgg
    15001 gcgaaacatt agagcagcga atagagcatt tagaagtagt tcgccgcatg caggatgaaa
    15061 caggtattat catgggcttt attccctggt cattttcgcc aaagggtacg tacatggatg
    15121 atataatccc tgcaactggc ctggattatc taaaaactgt cgccattgca cgaatttttc
    15181 tggacaatgt gccatatctt catgcaggat ggcttaccga ggggatggca ttagcacaga
    15241 ttgcacttgc aatgggtgca aacgatatgg gtgggatttt gatggaagag aaggtggtaa
    15301 aagcaacagg cgtaaccaca accacaaata ttgaacaaat atgtgatatc attattaatg
    15361 cggggaaagt tcccctgcag cgtgattcta agtacaggcc tatcaggaga tatgtatgag
    15421 atacagggtt gataatgata ctcctcatga gcgcaaagca aaaatacggt caatgtttga
    15481 ttccattgtt gatggctatg acagagcaaa ccgaatttta tcgttaggca tagacaggtc
    15541 atggcgtaaa acagttgtag agcactgtga atgcaaaccg tctgatgtgg tacttgatat
    15601 atgctgtggt actggtgatc tgtctgcatt aatccgtggt acaggtgcac atgttgtgtc
    15661 gcttgatttt tcagaaaaca tgattcaaaa aggaagagaa aagggaaata ttgatgacct
    15721 tgcaacagtt gctgatgctt caaaattgcc atttaaagac aatattttta ccaaagtatg
    15781 catatcattt ggcatccgca atattcctga tattgataat ttccttgaag aggtgtacag
    15841 ggttttacga cctggtggta cattcattat tttagaactt acccgtccac gctacaatat
    15901 attcagattg ctatactttt tttatcttta tactatttta ccagtgcttg gtggaatcat
    15961 tgctggaagt tttaatgcct ataggtatct ttcaaaaacc atacacacat ttattgaccc
    16021 tgttacattg acagccatgc aggaaaaaca tggctttatc gatataacat ataaatcgat
    16081 gacattgagc attgcaacaa tgtatatcag caaaaagcca cctgtgggga cgtcattcct
    16141 gtcaatacca ttatcaaaac aatcacatcc caataatgtc attattcatt aaaagtaata
    16201 taaatattta ttagttaatg ggtgattacc agaaatcagg gaagtaactt tatcctgtat
    16261 tatttgtgag atagttccag gaacaggtct aaagataata tcatcaatgc tttcaatagg
    16321 ctgtatgtta tttacgctgc cgctgaaaaa agcttctgaa aactgattga tgctttctgc
    16381 agggatatcg cattctgttg ccgggatgtt atcatggtga gctatatcta acacaatcat
    16441 tcgtgttatg cctggcatga ttgaacgcag agtaggtgta tagagactct gaccctttac
    16501 aaaaaatata ttggcggtgg cagcttcagc tatgttaccc tgagtatcaa caagtatggc
    16561 atcagaatac cccttttgtt ttgcttccat ttgtgctaag tagctattga cgtaattacc
    16621 tgcaactttt gcatatacgg gtactgtcaa agggtcaatt tttttatacg tgcttaagca
    16681 tgcacgggaa ggttttgaaa gctcatgcac atcaaaattc agagtttcgt aattgataca
    16741 gaatattgca agcgacacat tcattgaatg tggtacaatg gtaagttcaa tatcatcaaa
    16801 ataggcaaag aatttaacaa gaccattttt acaattattg gcctttgcac aggtaactat
    16861 cgcttcaatt atttgttctt tgttgtatgg tagttgtatg tgcaatgctc tggcactgtt
    16921 aaaaaaccgt tcacaatgct ggtgcaatcc cagcagggcg gtgccttttc cattggtaac
    16981 tatctccaac acttcaaata ttgctgtccc cctgctgaaa ctatgtgaaa gcagagaaac
    17041 ctttgcattg tgtatgtcag ttaaagagcc attgagccac gcaaccatga gccacacctt
    17101 ttattaacaa cattatatat cccgcaataa gtaaaaacgc ttgtaacaca tacgcgtgta
    17161 caaagtcaaa tcaataaaat atttttgatt ttaaactaat tatcgatagg ctaatttttg
    17221 caacgataga tagtttgaaa tggatttctg agaaaatctt tttggtagta atattagcgg
    17281 tagtaaaagg taaataagat tctacacaaa acatccttta gagtggatgt gtatagtttc
    17341 acaggaaaaa gaatgtagca ccctcataag aaatgtaact atataaagaa ataagtgaaa
    17401 agccaaagct aaacgttttt tgaaaacagc aaatgtggac aaaagcaaaa gtaaggggca
    17461 atggtagcca tacaggaggt acggcgtcat agggttgcgt gcgaggacat agccaggatg
    17521 gcgtc
//
LOCUS       DAORYL010000066        17223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_2704_length_17223_cov_13.260834, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000066 DAORYL010000000
VERSION     DAORYL010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 17223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17223
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2704_length_17223_cov_13.260834"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..1558
                     /locus_tag="PLV81_11345"
     CDS             119..1558
                     /locus_tag="PLV81_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10733.1"
                     /translation="MGYHVTIKKLCIIIALFFYLVQPVLAQESNAQSTDAQNYRNMNI
                     DMQVVYGQYNDMLSTVNLSQEQEGFVYLLRTYLKKSNDYGYKDEIYKNTSYYQNRIGF
                     TGNVTASETWKSIFDISVHNDSYGMAQNPVYSREEKDNVILDWKNIVKYSPSFEMYYG
                     IGGAQYYHRLVGTTTQGEKSRVYQGHGQIGGEYIWSASNRFKYLSTFYGYSYEQYEND
                     FFCNNEIVDDFNITKDVGVTIGLNADFNRDDHWLLGPIIGASYKGFSNSSFILQYSYF
                     MQPFKPEELYLQQKFIAPIYNLEPAHVHKVHAKIDYKMNSLITMQVVSQIEHSNNFYN
                     YVTVPGDVLTAQGIESWIVTNSVMLVLNIVPKVLNVDAQYTYAYYHADEHITYRPQHL
                     VAFAITYNGTKWKINLSHDIASSVYTSPVDDTKLPSRVVGYLDLQRKMLEGFFAYFRV
                     ENLYNNRYYLRENYPESGIKGLFGIRILL"
     gene            1597..2232
                     /locus_tag="PLV81_11350"
     CDS             1597..2232
                     /locus_tag="PLV81_11350"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOM10734.1"
                     /translation="MHSGTLIGVFDAVPTWVMLLPILLCSVIAVAAIVERIIFYRRIN
                     ADYALLMSSVYKECSTNNIQQALLLCKRVPGPISELIHNAITFASTKKEREGLIAGEV
                     YAAQKAIEKHIGIIATIATIAPMFGLLGTVTGMMKSFSALSKVGSVAQDLLAYGIAEA
                     LITTALGLLVAIPSWIFYNYLVSRVERFNKDLEYVANTLLDFPLCSIEQKQ"
     gene            2273..2698
                     /locus_tag="PLV81_11355"
     CDS             2273..2698
                     /locus_tag="PLV81_11355"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOM10735.1"
                     /translation="MDHGLKTTIKVKSKLGYRSLIDITSLVDMTFLLVAFFMVTSSFG
                     SLSSITVHLPKAVQSGQMQHATMVISINEKNEVFVNDVKYPLGDLLQEFRKRKESVKD
                     KVVIIRGDRDANYETIITVMDLLNQAGIPRFTLATVKPK"
     gene            2778..5300
                     /gene="bamA"
                     /locus_tag="PLV81_11360"
     CDS             2778..5300
                     /gene="bamA"
                     /locus_tag="PLV81_11360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOM10736.1"
                     /translation="MKKAGIFISVIVIVALFNGGFGQPSKYEGKIVKKIEFSGLLDVD
                     EEDLLDVMTTEVGYPLRAVEIREDIKKIFKTGKFENIIVETEEYQDGVAVRFVCTERP
                     AVNKIIFKGVEEVNETTLKEELPIKEGDVYRKDKVEAGLIKIRNKYETEGLFNAIVKY
                     KIEEAKDEKNAVNVIFIVDEGEDIKVQKIAIVGNTIVPLSTLKGLLQTEEEGLFADAK
                     FNKATYEDDKAKILAYYRELGYLDADIVEDTVEYRWLDPETQEKRGIYITIKLREGER
                     YYFDGYEVSGNEVFSTEDITSQFEQTRIGDPFNDTLYQKDRQMISFTYATKGYIFARI
                     IPERKITEKEVDVNGKKEKRKYVFTHFHIKEGSQAYIENIIIKGNQKTKDKVIRRELV
                     VYEGELFNSYKMQLSRERVYNLGFFKEVNFDVRPGSKEGYMNLIVDVVEQPSGTISLG
                     GGWGSMTGFSIFADVAENNFMGNGQRVSLRFEYGPLRNSITLGFNEPWLFDKPIGLNL
                     SVFYMLSTIEQSSIFTNSNQKAKHQTQQYGYSIGLNYRFWYYYGIGSVWYHAMKEVIN
                     ASGNSTDTVFLEQAQGLQEKRKLTLYAYRDSKDNYLNPTRGWRAEFSASFTGGYIIRG
                     DDHWVQYSPDLYWYFSPFHLPFLKTHPCVIELRFNGTFITPPWFKNKMYDVQNPDKNP
                     WLESEDRLYIGGPETLRGWEILDFDFPDSWRYGLFHRILYGAEFRIPIHPTFLWFVFF
                     FDAGSVWTDSFWEKSLEQSTRDIINEDKANGLLYDIRDINKIDYMSYFKYSYGFGFKI
                     QIPMMPLRFWFGRKLAWVGRDEGYFKPLSGFQFQFGIGDMRF"
     gene            5303..5746
                     /locus_tag="PLV81_11365"
     CDS             5303..5746
                     /locus_tag="PLV81_11365"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOM10737.1"
                     /translation="MKRFVLFILCLLIVSCQTVDREDSLTIRYVQLPILYNYMIQTSP
                     DALSLQKRYNELKAQLDVPSVSDAERKDLAVKLQSIADQMDKQKKIFLTDIQQAIATV
                     AKRKGYQIVLGGGDTVVYAKDGYDITSEILKELAALRLQKSPAAR"
     gene            5971..6642
                     /locus_tag="PLV81_11370"
     CDS             5971..6642
                     /locus_tag="PLV81_11370"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOM10738.1"
                     /translation="MHILKPVINIVLVFLLSCSEYGYYQKKGQELLKHYKGNYSIVIS
                     KKLFMLFVVSREGIVAKYHIGYGLNPDKKPKLYANDNRTPEGLYHITEILSMDAPKKT
                     SAYKTLLEMNKVYFKAKDGHYKFGHPDVDLGDNAYGPRFYRISYPNTDDLARYEEAKK
                     NGMLPQNKGKVPSIGSGIAIHGNNDPDSIGQLASSGCIRMYNDDIIELEQYIQVGTPV
                     LILPD"
     gene            6802..7110
                     /gene="yajC"
                     /locus_tag="PLV81_11375"
     CDS             6802..7110
                     /gene="yajC"
                     /locus_tag="PLV81_11375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOM10739.1"
                     /translation="MFPFEIAYAANGQQASSPWVSIVPLVLMILIFYLLLIRPTQKKE
                     KDRMKMIDQLKKGDKVLTSSGMIGVVVNIKPEENIITLKLAEGVKVDFVKSAIQQKLS
                     "
     gene            7149..>7340
                     /locus_tag="PLV81_11380"
     CDS             7149..>7340
                     /locus_tag="PLV81_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10740.1"
                     /translation="MKQCRNIFLVLCVACMVVLCSTVMHVYAQETPSTTVQSPENTPG
                     NETPVQELSPQIQKETPKTE"
     assembly_gap    7341..7440
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7441..7659
                     /locus_tag="PLV81_11385"
     CDS             <7441..7659
                     /locus_tag="PLV81_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10741.1"
                     /translation="ITIKENNEQILAHIEQEKQNIDTEEKGLFGLSKQASDRVAKIIL
                     VVIIIFVFILYRMRTRSTSGSVLRRFPK"
     gene            7684..9339
                     /gene="secD"
                     /locus_tag="PLV81_11390"
     CDS             7684..9339
                     /gene="secD"
                     /locus_tag="PLV81_11390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOM10742.1"
                     /translation="MTRGLLYKAIFIICIVCFAVMLILPTIGENTLQIVLSQTSTEQD
                     LQTIKQRFPESKYKIVINNKEVLVEGYNLNVAVMNEIKRYPGVVDAKLLPHWAEKALL
                     AKKINLGLDLQGGSYLVLQANYHKIEEKTGKPLTNAEKNEITEQALELLRNRVDKFGV
                     AEPSIRPKGNDAIEIQLPGVKDPESVKKAIGTTGRVEYRLVDDTYTQKANQWLKEHFA
                     GKPLPEDQLELNKLADTIKTAIALPDSLELLYYYERNPQTAKLFPAYPIVLEKKVALA
                     GNDISKAWVAPDEYQQLAVHFNTTPEGAAKFAEVTSEKNHGKKLAIVIDDKVRSAPSI
                     RVQINTGQAIIQGDFTYEEVTALARIIKEGALPVDLKIIEERTVGPTLGIDSIQSGVK
                     AFIVGIAGIMLFMLIYYKFAGLIADIGLLLNGIFMLALLSWLGFTLTLPGIAGFILTM
                     GMAVDANVIIYERIKEELRTGKTPRAAVAQGFERAFWTIFDSNITTLLSAFVLSQFGT
                     GPIKGFAVTLSIGIISSMFVALYITKFIFETITTRGSLKKLSI"
     gene            9351..10241
                     /gene="secF"
                     /locus_tag="PLV81_11395"
     CDS             9351..10241
                     /gene="secF"
                     /locus_tag="PLV81_11395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOM10743.1"
                     /translation="MEKIIPFIRYRFIAYAITITLIVIFSLQTYRNGGFNWGIDFVGG
                     IKLTVKFEQGVDTAQIRKVLTQNKISAVIQQVGKEEDNEYLISTKLASRAENVEESTK
                     ELKAVIQSNFKNVEILSIETVGPAIGDFLKKSAGKLIALSIIIMTLYLAFRFELKYSI
                     GAMVALVHDVILSVLFCGFAGIEINIPIVAAILTIYGYSVNDTIIIFDRVRENLQIKT
                     KQTFIDVIDKSITQTISRTLLTALTTLFAVFALYMISGEGLNEFASVLLFGIVIGTYS
                     SMYLASPFVLYWEKLTLKKA"
     gene            10246..11121
                     /locus_tag="PLV81_11400"
     CDS             10246..11121
                     /locus_tag="PLV81_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10744.1"
                     /translation="MPLRHIIILIVCIVASCNVFAQGLDNPYSYTAIAGFANQLVEQR
                     EYYRAYQEYQRLYYYFPSLYSYDAFLIASLYCHYQGKQYHSVLEKVKNQSPINLPVWI
                     YSFDSAFSGMLLPPQMMDVQWQKDVNPEYEAMILKRKLAYSLLTVDNYNELSPYTVLA
                     KEFDLQRIYQYSRVTRDKLKNPYAALGWGILPGAGYIYSDNTENGVIAAIVIGICAVV
                     TYFAVDSGNSGIAFVTGAAGTFFYGGSILGGYLAAKRTNNQIMKELHAYLEEELKFAQ
                     DRDIIFNRYGKPKTQ"
     gene            11066..12328
                     /gene="ribD"
                     /locus_tag="PLV81_11405"
     CDS             11066..12328
                     /gene="ribD"
                     /locus_tag="PLV81_11405"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOM10745.1"
                     /translation="MHRIETLFLTDMESQKHNEYMTLALKLAFGKLGVTSPNPAVGAV
                     IVKDDTILATGSTQACGLDHAEVVALKNATGNLAGATMYVTLEPCCHWGKTPPCTDAI
                     IKSGIAKVIIPALDPNPAVAGKGVRALQQAGIEVIFLREFSQPAIDLLRPFKKSILRH
                     RPFIIHKAAISLDGRTATTMGDSKWISSPVSRLLVHRLRACADTIIVGKATVEKDNPA
                     LTVRYTDFSQETEKFYKTKQYTAVGYSNSFINGLLEAWKTDVTHDLKKIVIGVPDKLQ
                     NSKLAGDDNAYFFTTHKEKDALVKREDYTIIAKLIDSGRLQFLQSDSSVDQIHELLDI
                     LHTKGSMIAVLEGGARVAGSFFDSGEFDQCMYFYSPMLVGKGKNVIEGKGRQFITESM
                     LLHDVTSVFLGHDILVSGYKESYQFEAV"
     gene            12329..12970
                     /locus_tag="PLV81_11410"
     CDS             12329..12970
                     /locus_tag="PLV81_11410"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOM10746.1"
                     /translation="MFTGIIEEIGVVQSVKQSANGKLISIHARTVIEGTKVGDSIAID
                     GTCQTVTAIGKDYFTVFASDVTLGITTLGSFKTGTQVNLERAMQPQGRLGGHLVQGHV
                     DATGKIVSLHALPEGLAISLSVPENISKFIVPKGSIAIDGISLTVVDKKDTIELYCIP
                     ETIANTTLAHKKIGDMVNVEVDIIAKYVYYMIQNIQGGDSSLEKKLKEEGFWV"
     gene            complement(13023..14069)
                     /locus_tag="PLV81_11415"
     CDS             complement(13023..14069)
                     /locus_tag="PLV81_11415"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOM10747.1"
                     /translation="MKPVVALIGCGRIGFLLENDPLRYKPCTHYGGTQAAKLPITHAC
                     DINEKRLNAFSKIAGIPQSNCYTDYQMLIEHEKPQCVIIASWTHTHAPIGIAAAQHGA
                     KVIVCEKPVASDLAQAKAFIDACNTYNTTLIVNYERRYDYRYNYVKKLISNNKIGCIS
                     SVHGFVFTPSKKHQPGSGGGPLLHDGTHLIDIVQYLFGSIAQVQGYTTRYSQDSLYED
                     YAIAHCITTSGIHVTLEAGGYRDYFMFELQIFGTRGRIVIGNGYLYLYTPKKSRFYKG
                     LNDLIASEVTPKGKPDYFVKLYKEAKQCLHKPVNVTSSGYDGYKALEVIQAIYLSANH
                     NGEKIMLPLNVELQ"
     gene            complement(14076..15335)
                     /locus_tag="PLV81_11420"
     CDS             complement(14076..15335)
                     /locus_tag="PLV81_11420"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOM10748.1"
                     /translation="MKFIRLWFTDILGFLKSFAITINELEDALDEGVRFDGSTIHGFV
                     RKTEKEMIAMPDVETFEILPWRPKSNSVARMFCYIYNPDMTPYECDVRYILYKNLKKA
                     SDKGYLFYVGPEIEFFLFKTSESPQLLDRGGYFDLTPLDIASDIRRQMVLTLEEMGIG
                     VKSSHHEGAPSQHEIDLRHDDALTIADNIMTFKTATKEIAQQNNIYASFMPKPLSQYN
                     GCGMHIHQSLFKNEVNIFYDSNDELMLSDIAKHYIAGLLKHAPEFFALSNQWVNSYKR
                     LSYGFEAPIELSWSINNPSSLIRITTSRTDKPNSIRVELRSPDPACNPYLLFSAILAA
                     GLSGIEHNDPLPKPHNLNEKKSGFTTKSLPSNLSDALKLFEKSSLMREALGDTLIELF
                     IENKFYELDQYNRHITDYEIDTYLPIL"
     gene            complement(15380..15508)
                     /locus_tag="PLV81_11425"
     CDS             complement(15380..15508)
                     /locus_tag="PLV81_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10749.1"
                     /translation="MFYLLLRLLYEEMAEGKISKEEYYEAVIRIASIHGIKRNGIR"
     gene            complement(15601..16593)
                     /locus_tag="PLV81_11430"
     CDS             complement(15601..16593)
                     /locus_tag="PLV81_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10750.1"
                     /translation="MAIESENNNLFSDHLIIIKGRFKALSMNLYGLFIIIFNQDRRID
                     TYNVAVSTFSNIYENEPHLPWDKYEQVILELKWKGNFNANLTTSIMDNFKEIANEDTK
                     TNQLYDYANNYDYDSIDTYLFDTINRVIHDKNLVLETSIQEVTAEEFAQTKEKRYTQP
                     EEKSSTAEAELEEGAVILNIQPILAPVKGKPIYELKVGDKIMTKIIPNTDRANYFIDL
                     LGLRVENHIKPIATEVIDIKSDGKNEPIEILTRIGPGIYGKCIEDERQVKLRLYDPII
                     DGPLTQKSIPKQTPKSPVASTTYEDAGFSKLTYVIISLFVLLLIIFVLLIYISF"
     gene            16811..>17223
                     /locus_tag="PLV81_11435"
     CDS             16811..>17223
                     /locus_tag="PLV81_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOM10751.1"
                     /translation="MKGMELLTDLYELTMMQVYMKTGIIQNHAIFDMFFRKNPFNSGY
                     VVFAGLEQLIDYLQNLSFSKESIDYLYSLHIFSDDFLDYCKSFRFTGSIFSFQEGDVV
                     FPEEPVIRVEAPLGQAQLIETALLNCINFQSLIAT"
BASE COUNT         5328 a         2973 c         3684 g         5138 t
ORIGIN      
        1 agcaaattcc ttaaattgta ttgacttaaa accgcatcat ataaataatc aaccttcatc
       61 acaagtaatg ttacagtata taaaaactat atttctatat acaaatatat ggggttgtat
      121 ggggtatcac gtaactataa aaaaattatg tataattatt gcacttttct tttatctggt
      181 gcaaccagtc cttgctcagg aaagtaatgc tcagagtact gatgcccaga actatcgcaa
      241 catgaatata gatatgcagg tggtgtatgg tcagtacaac gatatgctgt caacagttaa
      301 cctttcacag gagcaggaag gatttgtata tttactgcgg acatatttaa aaaaatccaa
      361 tgattatgga tacaaggatg aaatatataa aaacacaagc tactaccaga atcgtattgg
      421 gtttaccggg aatgtaactg cttcagaaac ctggaaatca atttttgata taagtgtgca
      481 taatgattca tacggcatgg cacaaaatcc cgtatatagc cgtgaagaaa aagacaacgt
      541 tattcttgac tggaaaaata tagtaaagta cagcccttcg tttgaaatgt attatggcat
      601 aggcggtgca cagtattacc acaggctggt tggcacaaca acgcagggcg aaaaaagccg
      661 cgtatatcag ggacacggtc agattggggg tgaatacata tggtctgctt ccaaccgttt
      721 taaatattta tctacgttct atggctactc atacgaacaa tatgaaaatg attttttttg
      781 taacaatgag atagttgatg actttaatat aacaaaggat gttggagtaa cgattggcct
      841 taatgcagat tttaacaggg atgatcactg gttgctgggg ccaattattg gagcaagtta
      901 taagggattc agtaattcca gcttcatatt acagtacagt tacttcatgc agccgttcaa
      961 accagaagaa ttatacttac agcagaaatt tatagcacca atatataacc ttgagccggc
     1021 gcatgttcat aaagttcatg ctaagattga ttataaaatg aatagcttaa taacaatgca
     1081 ggtggtatcg caaattgaac acagtaataa cttttacaat tatgtgactg ttccgggcga
     1141 tgtgttgact gctcagggta ttgagtcatg gattgttacc aatagtgtta tgctggtact
     1201 caatattgtg cccaaagtgc tcaatgttga tgcacagtat acctatgcat attatcatgc
     1261 tgatgaacat ataacctaca gaccacagca tctggtagcc tttgcaataa cctataatgg
     1321 aacaaagtgg aaaattaatc tttcccatga tattgcaagt tcagtatata cttcgcccgt
     1381 tgatgatacc aaacttccat cgcgggtggt tgggtatctg gatttacagc gaaaaatgct
     1441 tgaaggcttt tttgcatatt ttagagtgga aaatttgtat aacaatcgct attatcttcg
     1501 cgaaaattat ccggaatcag ggatcaaggg tttgtttggc atacggatat tattataggc
     1561 gatagttcat agtaaataat cacaggatga aataatatgc acagtggtac attaattggc
     1621 gtatttgatg ccgtgccaac atgggtgatg cttttaccaa tattgctatg ttcggtgata
     1681 gctgttgctg caatcgttga acgaataatt ttttaccgta gaataaatgc cgattatgca
     1741 ctgctgatga gcagtgtata taaagaatgc agcacaaaca atatccagca ggcattgctg
     1801 ttgtgcaaaa gggtgcctgg ccccataagt gagcttatcc acaatgcaat aacttttgcc
     1861 agcacaaaaa aggaacgcga aggacttatt gcaggcgagg tatatgcagc acaaaaagct
     1921 attgaaaagc atataggcat tatagcaact atcgccacca tagccccaat gtttggcttg
     1981 cttggaactg ttaccggtat gatgaaatca ttcagtgcct tatcaaaggt gggcagtgtg
     2041 gcacaggatt tgctggcata tggtattgcc gaagcgctta tcacaacagc attgggtttg
     2101 cttgtagcaa taccgtcatg gattttttat aattatctgg tcagccgtgt tgaacggttt
     2161 aataaagatc ttgaatacgt agcaaatacg ctgctggact tcccattatg ttcaattgaa
     2221 cagaaacaat aatatcagta ctgcgataaa aaagggatgc agtaaatcgc atatggatca
     2281 tgggttaaaa accacaataa aagtaaaaag taaattaggg tacagatcct taattgatat
     2341 aacgtcactg gttgacatga catttttact ggtagcattt tttatggtga catcaagttt
     2401 tgggtcacta tcgtccatta ccgtacacct gcccaaagca gttcaatcag ggcagatgca
     2461 gcatgccact atggttatca gtataaatga aaaaaatgag gtatttgtta atgatgtaaa
     2521 atatccactg ggggatttgt tgcaggaatt caggaaaaga aaggaatcgg ttaaagacaa
     2581 agttgttatt atccgcggtg atagggatgc aaattatgaa actatcataa ccgtgatgga
     2641 tctgctcaat caggcaggca taccgcggtt tacgctggca actgttaaac ccaaatgaaa
     2701 actgcatatg aaatatacta aacagtaccg tgaatggtaa atgctgtggt taaaaaagaa
     2761 taaaggaatt ggacaggatg aagaaagcag ggatttttat ttcagtaatt gttattgtag
     2821 cgctatttaa cggtggtttt ggacagccct caaaatatga agggaagata gttaaaaaga
     2881 tagagttttc cgggctttta gatgttgatg aagaagacct tctggatgtt atgaccactg
     2941 aagttggcta tcccctgcga gcggtggaaa tacgtgaaga tataaagaaa atatttaaaa
     3001 ccggaaagtt tgaaaatatt attgttgaaa ccgaagagta tcaggatggc gttgctgtcc
     3061 gttttgtatg tactgagcgc cctgcagtta ataaaataat atttaaaggc gttgaagaag
     3121 tcaatgaaac aaccttaaaa gaagaattgc ccatcaagga aggggatgta taccgcaaag
     3181 ataaggttga ggcaggcctc attaaaatcc gcaataaata tgaaacagaa ggcctgttca
     3241 atgcaattgt gaaatacaaa attgaagagg caaaagatga aaagaatgca gtcaatgtca
     3301 tatttattgt ggatgaaggg gaggatatca aagtacagaa aatagccata gtaggcaaca
     3361 ctatcgttcc attgagtaca ttaaaagggt tgttgcaaac cgaagaggag ggcctctttg
     3421 ccgatgcaaa attcaataaa gccacctatg aggatgataa agcaaaaata ctggcgtatt
     3481 accgtgagtt ggggtatctg gatgctgaca ttgttgaaga tactgtggag taccggtggt
     3541 tagatccaga aacacaggaa aagcgcggta tatacataac aataaagttg cgtgagggtg
     3601 agcgatatta cttcgatggc tatgaagtat caggcaacga ggtattttct actgaggaca
     3661 ttacttcaca gtttgaacaa accagaattg gcgatccatt taatgacacc ctttatcaga
     3721 aagaccgcca gatgataagt tttacctatg caacaaaagg gtatatcttt gcccgcataa
     3781 tccccgaacg caaaataacc gaaaaagaag tggatgttaa tggaaaaaaa gaaaaacgca
     3841 agtatgtctt tacccatttc catattaaag aaggctctca ggcgtatata gaaaatatca
     3901 tcatcaaagg taatcaaaaa accaaggata aggttattcg ccgcgaactg gttgtctacg
     3961 aaggtgagct tttcaactca tacaagatgc agctttcgcg ggagcgtgta tacaatttag
     4021 ggttcttcaa agaagttaac tttgatgttc ggcctggctc aaaagaaggg tacatgaacc
     4081 tcatcgtaga tgttgtggaa cagccatcag gaacaatatc acttggtggt ggctggggtt
     4141 ccatgacggg tttttcaatc tttgctgatg tagctgaaaa taactttatg ggcaatggtc
     4201 agcgtgtttc acttcgcttt gagtatggtc cactgcgaaa ttctattacg cttggcttca
     4261 atgaaccatg gctttttgat aagcccattg gtcttaatct ctctgttttc tatatgctat
     4321 caactatcga acagagctct atttttacaa attccaatca aaaagcaaaa caccaaacac
     4381 agcaatatgg ttattccata gggttaaatt acagattctg gtattactat ggcataggct
     4441 ctgtgtggta ccatgccatg aaggaagtta ttaatgcatc cggtaacagt accgatactg
     4501 tttttctgga acaggctcag gggttacagg aaaagcgtaa attaaccctg tatgcatacc
     4561 gtgattcaaa agacaattat cttaatccta cacgtgggtg gcgggctgag ttttctgcat
     4621 cctttactgg tggctatatc attcgtggtg atgatcactg ggtgcagtat tcacctgatc
     4681 tgtactggta tttttcgcca tttcatctgc ctttcctgaa aacacatcca tgcgttattg
     4741 agttacggtt taacggtaca tttataactc cgccgtggtt taaaaataag atgtatgatg
     4801 tgcaaaatcc tgacaaaaat ccctggcttg aatcggaaga caggctgtat ataggcggcc
     4861 ctgagacatt gcgtggatgg gaaatcctgg attttgattt tcccgattcc tggcgttatg
     4921 ggcttttcca cagaatatta tatggtgctg aatttagaat acccatccat ccaacgttct
     4981 tgtggtttgt attcttcttt gatgctggtt cggtgtggac cgatagtttc tgggaaaaga
     5041 gccttgagca atcaaccagg gacatcatca atgaagacaa agccaatgga ttgctctatg
     5101 atatcaggga tataaataag attgactata tgtcgtattt caagtattcg tatggatttg
     5161 ggtttaagat acagataccc atgatgccgc tgagattctg gtttgggcgc aaattagcat
     5221 gggttggcag ggatgaaggc tattttaaac cattgagtgg cttccagttc caatttggca
     5281 ttggagatat gcgattctga taatgaaacg gtttgtttta tttatactgt gtttactgat
     5341 agtgtcatgc cagacagttg acagggaaga cagccttaca ataaggtatg ttcagttacc
     5401 catattgtat aattatatga ttcagacatc gccggatgct ttatcattac aaaagcgtta
     5461 taatgagttg aaagcacagc ttgatgtccc atcggtaagt gatgccgaaa gaaaagacct
     5521 tgcagtaaag cttcaatcaa ttgcagatca aatggataag cagaaaaaaa tttttttaac
     5581 ggacattcag caggcgatag caactgtagc aaaacgtaaa ggataccaga tagtccttgg
     5641 tggcggtgac accgttgtgt atgcaaaaga cgggtatgat attaccagtg aaatacttaa
     5701 agagcttgca gcattgcgct tgcagaaatc acctgcagca cggtgatgtt ttaattcaac
     5761 tttgttggga cttgcaatga cgacagtagt acgtcactga gagcgaagcg aagcagtctc
     5821 aggtcaactg caatggatgt atgtcaaacg gcgagattct tcggccgccc caataaagtc
     5881 agggcgtctt cagaatgaca gagtgctatt gtattgggac ttgtaatgac atctacatat
     5941 aattccatgc aaaaggagta ttttatttgt atgcatatcc tgaaacctgt aatcaatata
     6001 gtattagtat tccttctttc ctgtagtgaa tatggatact atcagaaaaa agggcaggaa
     6061 cttttaaaac attataaggg taattacagc atcgttatca gtaaaaaatt atttatgtta
     6121 tttgttgtca gccgggaggg tattgttgca aaataccaca ttggatatgg attaaatcct
     6181 gataaaaagc caaagctgta tgcaaacgac aaccgtacgc cagaggggct atatcatata
     6241 actgaaattt taagcatgga tgctccaaaa aaaacaagtg cctataaaac tctgctggaa
     6301 atgaataaag tatattttaa ggcaaaggat gggcactata agtttggtca tcctgatgtt
     6361 gatttaggcg ataacgccta tggaccacga ttttatcgta tttcatatcc aaatactgat
     6421 gacctggctc ggtatgaaga agcaaaaaag aatggcatgt tgccacaaaa taaagggaag
     6481 gtgccatcaa taggtagcgg tattgcaatt catggcaata atgatccgga ttccattggg
     6541 cagctggcat caagcggttg catacgtatg tataatgacg atataataga attagagcag
     6601 tatattcagg ttggtacacc ggtattaata cttcctgatt aaaaatattg cataaattgt
     6661 gttgacattt gcaattcatt gcataatggt tgcagtgatt ttatctgtgt atttattgag
     6721 taaaagcata tcaggctttc tatgggtctg aacatgcttt gtgtgaatct attaactaaa
     6781 acataaaagt gaggtaacaa cgtgtttcca tttgagatag catacgcagc caatggacaa
     6841 caggcatcaa gtccatgggt aagcatagtc ccgcttgtct tgatgatatt gattttctat
     6901 cttcttttga tacggccaac acaaaagaag gaaaaagacc gtatgaagat gatagaccag
     6961 cttaaaaaag gcgataaagt cctgacatca agcggaatga ttggcgtggt ggtaaatatt
     7021 aaacctgaag aaaatatcat taccctgaaa cttgcagaag gcgtaaaggt ggactttgtc
     7081 aaaagtgcta ttcagcaaaa gttatcgtga tgtattaatg ataaaatggt attgacagga
     7141 acaatagtat gaaacagtgc cgaaatattt ttctggtatt gtgtgttgcg tgtatggtgg
     7201 tgctgtgctc tacagtgatg catgtgtacg cacaggaaac accgtccact actgttcagt
     7261 caccagagaa tacaccgggc aacgaaactc ctgtacagga actatcgccc cagattcaaa
     7321 aagaaacacc taaaactgaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 attaccatta aagaaaataa tgaacagata cttgcacaca tagaacaaga aaaacagaat
     7501 atagatactg aggaaaaggg tttatttggt cttagcaaac aggcatctga ccgtgtggca
     7561 aaaattattt tagtagtgat cattattttt gtttttatcc tctataggat gaggaccaga
     7621 agtacttcag gtagcgtttt gcgcagattt ccaaaataat acataataat gcatgaggtg
     7681 tacatgacac ggggattatt gtataaagct atttttataa tctgtattgt gtgttttgct
     7741 gttatgctca ttttgcctac cattggggaa aataccctgc agatagtatt aagccagaca
     7801 tctaccgaac aggatttaca gacgataaag cagcgatttc ctgaatcaaa gtataaaatt
     7861 gtgattaata ataaagaagt actggttgaa gggtacaatc ttaatgtagc agtaatgaat
     7921 gaaataaaac gctaccctgg cgttgtggat gccaaacttt taccacactg ggctgaaaag
     7981 gcgctgcttg caaaaaaaat aaatttaggt cttgatttgc agggtggctc atacctggtg
     8041 ttgcaggcaa actatcacaa gatagaagaa aaaacgggca agccactgac taatgctgaa
     8101 aaaaacgaaa ttaccgaaca ggcactggaa ttgcttcgta accgtgttga caagtttggt
     8161 gtagccgagc cttcaatacg gcccaagggc aacgatgcca ttgagattca gttacccggg
     8221 gttaaagatc cggaaagtgt aaaaaaagca attggcacca ccgggcgtgt tgagtaccgg
     8281 ctggttgatg acacctatac ccagaaagcc aatcagtggc taaaagagca ctttgcaggc
     8341 aaaccgttgc ctgaggatca gttggaactc aataagcttg ccgacacaat taaaactgca
     8401 atagcacttc ctgattcctt agagttgtta tattattatg aacgcaatcc gcaaacagcc
     8461 aaattatttc cggcataccc aatagtcctt gaaaagaagg ttgcactggc aggcaatgac
     8521 ataagcaagg cgtgggttgc acctgatgaa taccagcagt tagcagttca ttttaatacc
     8581 acaccggaag gtgctgcaaa atttgcagaa gttaccagtg aaaaaaatca cggcaaaaaa
     8641 cttgccatag ttattgatga taaagtccgc agtgcaccat caatcagggt gcagattaat
     8701 accgggcagg caatcattca gggtgatttt acctatgagg aagtaaccgc acttgcacga
     8761 attattaaag aaggtgcatt acctgttgat ttaaagataa tagaagagcg aacggtaggc
     8821 ccaacacttg gtattgattc aattcaaagc ggtgtaaagg cgttcattgt tgggattgca
     8881 ggtataatgc tatttatgct catttattac aaatttgcag gactgatagc cgatataggc
     8941 ttgcttctta atggcatatt catgttagcg ttactatcgt ggttaggatt tacactaacc
     9001 cttcccggta ttgctggttt tattttaaca atgggtatgg cagtagatgc caatgttatt
     9061 atctatgaac gaataaagga ggaactgcga actggcaaaa caccccgtgc agcagtagct
     9121 cagggttttg aaagagcttt ctggaccatc tttgactcaa atatcaccac attgctttcg
     9181 gcatttgtgc tgtcgcaatt tggaaccggt ccaattaaag ggtttgcagt aaccctttca
     9241 ataggtatta ttagttctat gtttgttgcg ctgtatatta caaagtttat ttttgaaaca
     9301 ataactacac gtggatcact gaaaaaatta agtatttaag gagtggcacc ttggaaaaga
     9361 taattccttt tataagatac agatttattg cctatgcaat tacaataaca ttgattgtga
     9421 tattttcatt gcagacctat cgcaatggtg gttttaactg gggtattgat tttgttggtg
     9481 gtattaaact tactgtaaag tttgagcagg gggttgatac cgcacaaata cgtaaggttt
     9541 tgacccagaa taaaattagt gcagtgattc agcaggtggg gaaagaagag gataatgaat
     9601 atctgatttc caccaaactt gccagccgtg ccgaaaatgt tgaagaaagc accaaagaac
     9661 tgaaagctgt tattcagagt aattttaaaa atgttgaaat attgagtata gaaactgtgg
     9721 gtcctgcaat tggtgatttt cttaaaaaat cggcggggaa actgattgcg ctttcaatca
     9781 taatcatgac gttatattta gcattccggt ttgaattgaa gtattccatt ggtgcaatgg
     9841 ttgcactggt acatgatgtt atactatcag ttcttttctg cggatttgca ggtattgaga
     9901 ttaatatacc gatagtagcc gcaattctta ccatttatgg atactcggta aacgatacca
     9961 ttatcatatt tgaccgtgtg agagaaaatt tacagattaa aaccaagcaa acatttattg
    10021 atgtgataga taaatctatc acacagacaa tatccagaac gctgcttact gcgcttacca
    10081 cattgtttgc cgtatttgcc ctgtatatga ttagtggaga aggccttaac gaatttgcca
    10141 gtgtactgtt gtttggtata gttataggaa cgtattcatc tatgtattta gcatctccgt
    10201 ttgtgttata ctgggaaaaa ctgacattaa aaaaagcgta agcgtatgcc gttgcggcat
    10261 attattattc tgatagtgtg tattgtagca agctgtaatg tctttgccca aggattagat
    10321 aatccctata gttatactgc tatagcaggg tttgcaaatc aactcgttga acaaagggaa
    10381 tactaccgcg catatcagga ataccagcgc ttatactact acttcccttc gttgtattcg
    10441 tatgatgctt ttcttattgc ttcattgtat tgccattatc agggcaaaca ataccattca
    10501 gttcttgaaa aagtgaaaaa tcaatcccca ataaacctgc cggtgtggat ttatagcttt
    10561 gacagtgcat tcagcggtat gctgttgcca ccacagatga tggacgtgca atggcaaaaa
    10621 gacgtcaatc cggaatatga agcaatgata ctgaaaagaa agcttgcata ttcattgttg
    10681 acagttgata attacaatga actatcgccc tatactgttc ttgcaaaaga gtttgatctg
    10741 caacgtatat accagtacag cagggttaca cgggataagt taaagaaccc ctatgctgca
    10801 ttggggtggg ggattcttcc tggagcgggt tatatataca gtgataatac agaaaatggc
    10861 gtaatagcgg cgatagttat cggtatatgt gctgtggtta catactttgc agtggatagt
    10921 ggcaacagtg gcattgcgtt tgttaccggt gctgccggca cattttttta tggtggcagc
    10981 atactgggtg ggtacctggc agcaaagaga acaaacaacc agattatgaa agagttacat
    11041 gcatacctgg aagaagaatt gaaatttgca caggatagag acattatttt taacagatat
    11101 ggaaagccaa aaacacaatg aatatatgac gctggcactg aaattggcgt ttggcaagtt
    11161 aggggtaacg tcgccaaatc ctgcagtggg agcggttatt gttaaggatg atacaatact
    11221 ggcaacggga tccacgcagg cctgtggcct ggatcatgct gaggttgttg ccctgaaaaa
    11281 tgcaacggga aaccttgctg gtgctacaat gtatgtgacg cttgagccat gctgtcactg
    11341 gggtaaaacc ccaccctgca ctgatgcaat aataaaatct ggtattgcaa aagtaattat
    11401 tcctgcactt gatccaaacc ctgctgttgc tggcaaaggg gtcagagcat tgcagcaggc
    11461 aggcattgag gttatctttt tacgtgagtt ttcacagccg gcaattgatt tactgcggcc
    11521 gtttaaaaaa agtattttgc gacacaggcc attcattatt cataaggcag caatatcact
    11581 tgacggcagg acggcaacaa ccatgggtga ttcaaaatgg ataagttcac ctgtttcacg
    11641 gcttctggta caccgactgc gtgcatgtgc tgataccatc atagttggta aagctaccgt
    11701 tgaaaaagat aatccagctc ttaccgtgcg ctacactgat ttttcacagg aaactgaaaa
    11761 attttataaa accaaacagt atactgcggt gggatattcc aatagcttca tcaacgggct
    11821 tcttgaagca tggaaaacag atgtaactca tgatttaaaa aaaatagtga taggagtgcc
    11881 agataaattg cagaatagta aacttgctgg cgatgacaat gcttattttt ttacaacaca
    11941 caaagaaaaa gatgcgcttg tgaagcgaga ggattatacc attattgcta agcttattga
    12001 tagtggcaga ctacagtttt tgcaatcaga tagcagtgtg gatcagatcc atgagttgct
    12061 tgacatattg catacaaagg gttcaatgat cgcagtgctg gagggagggg cacgtgttgc
    12121 tggatcattt tttgattcag gtgagtttga tcagtgcatg tacttttaca gcccaatgtt
    12181 agttggtaaa gggaaaaatg ttattgaagg caaaggcagg cagtttatta cagagtccat
    12241 gttgcttcac gatgtaacca gtgttttttt agggcatgat attcttgtat caggatataa
    12301 agaaagttat cagtttgagg cggtgtaaat gtttaccggc attatagaag aaattggcgt
    12361 ggtacaatcc gtaaagcaat cagcaaacgg gaaattaatt tccatacatg cgcgcacagt
    12421 tattgaaggc actaaagttg gtgattccat tgccattgac ggaacttgtc aaactgtgac
    12481 agcaattggc aaagattact ttaccgtgtt tgcttcggat gtaacacttg gcataactac
    12541 acttggttcg tttaagactg gaacacaggt caatctggaa cgagccatgc agccgcaggg
    12601 acgtctgggt gggcatctgg tgcagggaca cgttgatgca accggaaaaa ttgtttcact
    12661 gcatgcattg cctgaggggc ttgcaatttc cttatcagtg ccggaaaaca tcagtaagtt
    12721 tattgtgccc aaaggctcaa ttgccattga tggcatatca ctgacggtgg tggataaaaa
    12781 agacaccata gagctttact gtattccgga aactattgcc aatactacgc ttgctcataa
    12841 aaaaattggg gatatggtca atgttgaagt tgatattatt gcaaagtatg tttattatat
    12901 gattcaaaat atacagggtg gcgatagttc actggaaaag aagcttaaag aagaagggtt
    12961 ctgggtatag tataaatttt taattaagtt gggaacaggt agtaatgtta tttcagtact
    13021 attcattgaa gttctacatt gagtggcagc ataatttttt caccattatg gtttgctgat
    13081 agatagatag cctgtatcac ttcaagagct ttatacccat catacccgct tgaggtaaca
    13141 tttactggtt tgtgtaaaca ctgttttgct tccttataca gtttaacaaa ataatcgggt
    13201 ttgccttttg gtgtaacttc agatgcaatc aggtcattga gtcctttata aaatcttgat
    13261 ttctttggtg tatagaggta caggtagcca ttaccgataa ctatcctgcc cctggtgcca
    13321 aatatctgca attcaaacat aaagtaatca cggtaaccgc cagcttccag tgttacatgg
    13381 atgcctgagg tggtaataca atgagctatc gcataatctt catatagtga atcctgtgaa
    13441 tagcgtgtgg tatatccctg aacctgtgct atgcttccaa aaagatactg cacaatatca
    13501 attaaatggg tgccgtcgtg taaaagaggt ccgccaccag atcctggctg atgctttttt
    13561 gacggggtaa agacaaagcc atgcaccgat gatatacaac caattttatt gttgctaata
    13621 agttttttta cataattata gcggtaatca tatcgtcgtt catagttaac aatgagtgtt
    13681 gtattgtatg tgttgcaggc gtcaatgaag gcttttgcct gtgcaaggtc actggcaact
    13741 ggtttttcac atacaataac ttttgcgcca tgctgtgctg cagcaatgcc aattggcgcg
    13801 tgggtatgtg tccaggatgc aataataaca cactggggct tttcatgttc tataagcatc
    13861 tggtaatcgg tataacagtt tgattgaggg atccctgcaa tctttgaaaa agcgttcaaa
    13921 cgtttttcat tgatatcaca ggcatgggtt atgggaagct tagcagcttg tgtaccgcca
    13981 taatgggtac atggcttata tcgtaaagga tcattttcca gaaggaatcc aatcctgcca
    14041 caaccaatga gagcaactac aggtttcatg aaaccctata aaatgggtag ataggtatca
    14101 atttcataat cggtaatgtg gcgattgtac tggtcaagct catagaactt gttttctata
    14161 aataattcaa tcagtgtatc gccaagtgct tccctcataa gactgctttt ttcaaatagt
    14221 ttcagtgcat cagaaaggtt agatggtaat gactttgtgg taaaacctga ttttttttca
    14281 ttgagattat gaggttttgg cagggggtca ttgtgttcaa tgcctgaaag ccctgcggca
    14341 agaatagctg aaaacagcag atatggattg catgcagggt cggggcttct cagttcaact
    14401 ctgattgaat tgggtttatc agttcttgat gtggtaatgc ggataagtga tgatggatta
    14461 ttgatgctcc aggacagttc aatgggtgct tcaaatccgt aactcaaacg tttatatgaa
    14521 tttacccatt gattggataa cgcaaaaaat tcaggcgcgt gtttcaataa tcctgctatg
    14581 taatgctttg cgatgtcaga aagcattaat tcatcattac tatcgtaaaa aatatttact
    14641 tcatttttaa ataatgactg atgtatatgc atgccacagc cattatactg tgacagtggt
    14701 tttggcatga atgaagcata tatattattt tgctgtgcta tttccttggt tgccgtttta
    14761 aatgtcatga tattgtcggc tatggtcagg gcatcatcat gccgaagatc gatctcgtgc
    14821 tgactgggtg cgccttcatg atgggatgat tttacgccaa tgcccatttc ttccagtgtt
    14881 agtaccatct ggcggcgtat atcgcttgca atatcaagag gagtcaggtc aaaataaccg
    14941 ccacggtcaa gtagctgggg tgattcagaa gttttgaata agaagaattc aatttctgga
    15001 ccaacataga ataaatagcc cttatcggat gctttcttaa gatttttata cagtatgtac
    15061 cgaacatcac attcataagg ggtcatgtcg ggattgtaga tatagcagaa catccgtgcc
    15121 actgaattgc tctttggccg ccatggtaag atttcaaatg tttcaacatc gggcatggct
    15181 atcatttctt tttcggtttt acgcacaaaa ccatgtattg tagagccgtc aaaacgcact
    15241 ccttcatcca gggcatcctc aagttcgtta atggttatag caaaactttt taagaaccct
    15301 aagatgtccg taaaccataa tcgtataaac ttcacatcat tatcatgtgc cgtttttaaa
    15361 atatattcgc ggttgtccac tatcgtatac cgttcctttt tattccatgt attgaagcta
    15421 ttcgtataac agcttcatag tattcttctt ttgaaatttt tccttcagcc atttcttcat
    15481 acagcagtct caggagcaga taaaacatat accgcctcct ttgtattaat gtgtgctgct
    15541 gttgtattaa tcggcttgaa ggtttaatga aattagagga agtatagcgt attttgtcaa
    15601 ttaaaagctt atatatataa gtaaaacaaa aatgattagc aaaagcacaa acaatgatat
    15661 tatgacatag gttaactttg aaaaaccagc atcttcatac gttgttgaag caactggaga
    15721 ttttggcgtt tgttttggta ttgatttttg tgttaagggg ccatcaatga tgggatcata
    15781 aaggcgcaat ttaacctgac gttcatcctc aatacacttg ccataaatac cagggccaat
    15841 acgggtaagt atttcaatgg gctcattttt cccatcagat tttatatcta tgacttctgt
    15901 agctattggc tttatatgat tctcaacacg taaccccaat agatcaatga aataatttgc
    15961 cctatcggta ttgggtatta tttttgtcat tattttatca ccaactttta attcatagat
    16021 aggtttgcct tttactggtg ctagtattgg ctgtatattc aatataacag cgccttcttc
    16081 cagttcagct tcagcagttg atgatttttc ctcgggttgt gtgtaccttt tttcttttgt
    16141 ctgggcaaat tcttcggcag ttacttcctg aatgcttgtt tccagtacaa ggtttttatc
    16201 atggataacc ctgttaatgg tatcaaatag atatgtatca atactatcat aatcataatt
    16261 gttggcataa tcgtagagct gatttgtttt ggtatcctcg ttggcaattt ccttaaaatt
    16321 atccattatt gaagtggtta aatttgcgtt aaaattgcct ttccatttta attccaggat
    16381 tacctgttca tatttgtccc atgggagatg tggttcattt tcatagatat tggaaaatgt
    16441 tgaaacagca acattgtatg tatcaatccg cctgtcttga ttaaagataa taatgaaaag
    16501 cccatataaa ttcattgaaa gggctttgaa acgtcctttt ataataatta aatggtcact
    16561 gaaaagattg ttattttcgc tttctatagc catacattat tccacacaaa aaatatttat
    16621 atatagtaac tatcggcatg tatatatatt ttactataag tcttgataac tatcgcctta
    16681 atgaaaaaat attgtattca ataccatgac atagataata atattgtcaa tataaaaatt
    16741 ttttatttga ttgattttac atgtatatta ttataaatct ccttagggga atacagcaat
    16801 gaggaataca atgaaaggga tggaactgct gaccgacctg tacgaattga caatgatgca
    16861 ggtctacatg aaaactggca taatacagaa ccatgctatt tttgatatgt tcttcagaaa
    16921 aaatccattt aattctggtt atgttgtgtt tgccggatta gagcagctta ttgactatct
    16981 acaaaacctt tcattctcaa aggaaagcat tgattatcta tattcattgc atattttttc
    17041 tgatgatttc ctggattatt gcaaatcgtt tcgttttact ggcagcatat tttcatttca
    17101 ggaaggcgat gtagttttcc ctgaggaacc agtaatcaga gtagaagcgc cattaggaca
    17161 ggctcagctt attgagacgg cattgcttaa ctgcataaat tttcagtcgc ttattgcaac
    17221 aaa
//
LOCUS       DAORYL010000067        16430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_2871_length_16430_cov_14.593282, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000067 DAORYL010000000
VERSION     DAORYL010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16430
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2871_length_16430_cov_14.593282"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..1110
                     /locus_tag="PLV81_11440"
     CDS             73..1110
                     /locus_tag="PLV81_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10752.1"
                     /translation="MRKVHYICIIIMLWLPCRVNAGYYPFILTTTPVTCHQCKHFDNS
                     LYQSLVYTLTASGIFIGDSSSYSIPAGAYNPPGNNQLLRNLSIDITYSRKIHYKELTT
                     YLKEKVETESYTIAITIASSENPQEFTALYPNISPHQLEHTFNSIAQDILQFYSSKRI
                     PVLYQSPFAFYVTGLTLAPVYSILHDKLNNYAHSATGFQCLFTINNTYIPLTIMPSIT
                     WFTLNNPIKTINSWHSVSFMMHAGYQQALPFSVSITPYIGAGYILHYIEGDKTHTYPP
                     YSYSHSFYYNPQVATGIIFELILDPQLSLEAGAAYNMFFASSSQFSYLQYSFGFTFHG
                     NILLYQATPYH"
     gene            complement(1119..1721)
                     /locus_tag="PLV81_11445"
     CDS             complement(1119..1721)
                     /locus_tag="PLV81_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10753.1"
                     /translation="MDGKVKRSIIINRYYQLKTGFGVAGLFALCISILFIITGSFVMW
                     NNRSISRAMAHQLDLQNVQDDILKSIEMLSQYGNRKNLIFEAYKAIGDLQKNASALKE
                     SNELLTRSIAINRYLMWINGVLGITLVVVTFIIVVRRTHRVAGPMFLLKRYMNEIIEG
                     KVPEIRPLRKGDEFKDVFDTFTKMVESLHLKHRKNFKQKK"
     gene            complement(1740..5006)
                     /locus_tag="PLV81_11450"
     CDS             complement(1740..5006)
                     /locus_tag="PLV81_11450"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOM10754.1"
                     /translation="MSKNAIISFTIIDNGHIDISSIAQGVIIKDSSGQNIEYTYTYIA
                     ENKEIRCTPIQSLIEGQVYLVSVTDALHDEAGNPLENPYTWQFTVQVIPPQVLSINPA
                     DGQTNVSIGEYIRIVFSEPVMESTLSQGIKIEDSYATVPGTIQYNSETSTVTFIPDHL
                     EFFTDYIITVTTDIRDINGIPLDRNYTSTFRTMQENIQPEIISITPASPIGVETVFQI
                     QFSEPMDEGTLYSSVLLCDSYGNYFPVTIDYDEQTYCCTVTPDRYLTGFYQYIVEITT
                     GATDIHGNPLIQQYSYTYSTNPAPTDTVVLVYMPADYAGGDYIAGDISEMIQASGSID
                     PSVMRIVGIADYPYDTNTQLFEGIYGNRRDISLQTTGFVGNELNTAQAQTVSQLLSFV
                     NSNYLPQQIIFVLLDRKTKPIWCIAEDETSNARLSIHDFADAVRNNNISVLVLDAPIK
                     STVEVAYELRNSKTGIDYIIASQGEVLSGGFNYMALLNAVYTRITAELPPDNMTLAIA
                     QDICSTTAYPVSAGEVDTRSLALIDERQIESAISNMNAIISECQNIYDTSPNIIDAAR
                     FAAHFYYSIPWYADLVEFLQSTDNVQLQTVAGNFANCVLKKVMPLANIHSGLSIMFTT
                     KTHESWYISNLYSMDFVRDYSWDEFLQDKHIGLYIDPNELFNNEPDGYTVMLVDGNTD
                     SITINCQNYFHDPYDADIYSIDFNYRGGIYRLTDNTVNTINSNNDRWQSITASQDGAK
                     LAAAVYDGYIYTSGDGGTTWVQRNTPKSWTNITGSQDGTRLAATVYGGYIYTSGDGGT
                     TWTQRSIGPKNWISVACSQDGSKMVAAAYNDYIYISTSGGNSWTSIANPGQKNWSGVA
                     IAYDGTKIAATSYDGIYIITNQSGSWVASDIYNLSANWISIAVCSIGNDFIIVAAQNP
                     GYIYLSKVVNGVRVWTCLTQAGRRNWKSVSISPDSIRIIAIADNTIFESVDQGQTWYV
                     RNDAGMHRWNFVTYAWNQNALALVSSPANSAVNGLLTVAVNNVPENCEYIVRIADDNG
                     NILNESDRSGPGGTITLQQYIQTNVFRHYFISLIPVNVSGWSMNTKEQYAVQFNYQKN
                     "
     gene            complement(5003..5521)
                     /locus_tag="PLV81_11455"
     CDS             complement(5003..5521)
                     /locus_tag="PLV81_11455"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOM10755.1"
                     /translation="MIDQIFKSSKLLYHFIIFLWITLTIIVLPGCKNNNSSHQPINPD
                     GMQDYLTIISHTPLNNEVVFADSTVSVTFSHEIDMSTVNASTFYVLDERSLSVNGTYE
                     YIPASKTLRFIPVHGFEWGSTYTVYVTTGIYNTLGLHMQQEYSGRLLLLHFMIHRNPV
                     LMYHRCNPWEIM"
     gene            5849..6406
                     /locus_tag="PLV81_11460"
     CDS             5849..6406
                     /locus_tag="PLV81_11460"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOM10756.1"
                     /translation="MSEIKVGEKIKEFREAKGMSLHELALKTGFSSAFISQIENHLIS
                     PPLGALIKISHALNLEMGRFFGQTTEAPFTIVRSSERQLTSRVASKEGVRYGYTYESL
                     APEKVNRHMEPFIVTLEPVTGGQPYSHEGEEFIYVLEGQVKIQLADYSDILYPGDCIY
                     YDSIVPHRVDTATDSPAKILAVIYS"
     gene            6440..8137
                     /locus_tag="PLV81_11465"
     CDS             6440..8137
                     /locus_tag="PLV81_11465"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOM10757.1"
                     /translation="MHLSSNEILTLLLSISIMLIIAKIFSELFIRFKMPAVIGQIIAG
                     ILLGPTCTGSIAPNFFTWLFPSTGLLKGIIFLGLIFIMVKVGIEVELSSIYKQGRSIT
                     AVSFFSILIPFVAGFIASWYIPSFSGTYKSLPSSIFFAIVTSITALPVIAKILLDVKI
                     FHSDLGMIILTSAMVNDITGWILFSSLIKTVETGSIAIISIITSLGLTLLITIIILTI
                     FRYVLNELLPIVQAHTEWPGGVITLGLVLSMFLASLTEAIGIHAFLGAFMGGIILGDS
                     PHLNAHTKEIINQFIDNFFSPLFFVSIGLGINLITQLSLIQAFMITLVVFISNIIASL
                     IAGKISNNTFIDSLAIGVGMSTSGTMGIIVGIFALQYGIINTTTYSSIVVMAIATSLL
                     AGPLLKLILKPKEEVSLIELLDNNFIPECTCTNRNELIQQVSQHIQKRYGYDANKVAH
                     AIIEREQLMSTGIGQYVAIPHARIPECKKPVVICATHPQGLDFDAPDGKPAKVIFMII
                     TPQHDNDVQIFIISKIAQLCSNPNVVLQILNAKNNNEFVAILKTTSHIMEMEGYGTA"
     gene            8124..10385
                     /locus_tag="PLV81_11470"
     CDS             8124..10385
                     /locus_tag="PLV81_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10758.1"
                     /translation="MELHDIIKNDDDRLFIISPECFIVFTGDSTEDEKPFIRIGNSLL
                     LTPRVIPLIENIIITDLITGNPACEQYNIDPRYLSSNRYIGSKLIVKRYLEFQKLFGL
                     DLNNATIVDIEQDIPQLSKEQIISDRETFLGVFYTDSNFKILHNQKTMFDLKEIHQKY
                     PGDVLVHSKLSEYSNKSRYAGCGFVITRGATIFYKNNSFSTVGIPSRYYSTFAQLQVD
                     PANIRDVIITNTNSMPLVPLIKWKNAAGGRLRIFYDNDDEIKLLQKLFNQCTLHHKSS
                     KNFVCDNPEGITIQNIASSHNCIITIKNVKPATKDITIVYIHDPSGITQAIETAANLY
                     IIDYEIYKKAAMLCASLSPVIIVDSNRQGVPIKDVTYCIPSHQYDVRYYLDEKKLLSD
                     MLSCCSKEFAQAISEEDFDEIEKLLTQELSPHIVYNCIQTLRVILHSTTNRDLYKRIE
                     KILYKMQTRPLPDSYRYTIMLHKSYAYMTCQPVQVPQEYPFEAIEQLDEPSRPAPHTL
                     PDICNRIIEDRKRLEMLLDLFYANNTEIAKEAKSIEKAINKRKKEITQTPKLDVSLIT
                     KEKLKKRLTVLKKAGIIVAGIAVAILIMVGSYHAFILYQEKQERERQARYIEYLSKKY
                     AIHISDRDIFFYANDVAVKNGYSRIDIKSLKEKNPHWIYPGNRFILLDGEVVIVKPGD
                     TLWGISERKLLSLNITFYTLLDELEKKIDKGIIDEHLAQKAMNAAIHTKHRERVKALL
                     QKMNELKAKQNNT"
     gene            10403..10990
                     /locus_tag="PLV81_11475"
     CDS             10403..10990
                     /locus_tag="PLV81_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10759.1"
                     /translation="MEVKDPINYYVEPVEIEIYLKKAGKVRTIIKDMYIELIDPEPLD
                     NDTSKKIFDYFKSRNEPIDVIEITNIFPELISIVFESYYHNINLYEKLSMYFKAGLSG
                     SADSWRLALYFTELLMKFEPTIASSQHIGDFQTYNLNYCIRKLNALGEKFLLEDSTVM
                     YLIKRRNKAYEGKPKDKEFEKLVELWQFNVKERPF"
     gene            11068..11847
                     /locus_tag="PLV81_11480"
     CDS             11068..11847
                     /locus_tag="PLV81_11480"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HOM10760.1"
                     /translation="MKNPFYIIYDPAYTTSYVTASVEKPERVRQIMKELGDSYPIIKP
                     TSCTDEDILLCHSEGLLTMEKQDDVRFTVAKLAAGGAITAATMAMEGKPSFAVIRPPG
                     HHANPDHNWGFCYFNNMGIAIKRLLADSIIQKAIILDIDLHFGDGTDTIFKSTINVPV
                     LNIQSSNPVDFINETKTRLQQCGSADILGISAGFDQYIKDWGANLSTQDYFTIGQIAG
                     NHARHYCKGHIFAILEGGYYIPDLGKNVIALIEGICNGLYD"
     gene            11864..12796
                     /locus_tag="PLV81_11485"
     CDS             11864..12796
                     /locus_tag="PLV81_11485"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOM10761.1"
                     /translation="MHHKITISLFIKFFLYNTFVSGTICLFLFINKAISFTIFTVISF
                     FVINIVAACIAVSFIAKPFTMLLRSIQSLVNDRYTSSNPCSKEHLIMLTQELEQFKAR
                     SDSLLREKDTIVNELIESKKLLEKYSNNLEEMVEQRTEILKWLSITDPLTGLYNRRYF
                     IEQIELEFKRSKRYNRHLSLLMLDIDHFKSVNDNYGHQVGDIVLRKISSIIISQLRDS
                     DLAFRYGGEEFMVILPETKADDAINVAKRMKQEIMETEHIYRNLNFKVTASIGIVSIK
                     DMLGKFETVDDIIKKVDDNLYKAKNSGRNTIIYE"
     gene            complement(12797..13675)
                     /locus_tag="PLV81_11490"
     CDS             complement(12797..13675)
                     /locus_tag="PLV81_11490"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOM10762.1"
                     /translation="MSKLTVTFWGVRGSIPVPGPDTMKYGGNTACLDLRSDAGDWIVF
                     DAGTGLRVLGESLDLSKNYTVNLCISHPHWDHINGFPFFPVIYIPGNTVNIYGPGTFE
                     KSLEEIIRGQMQYSYFPVRTDELRASIHFYEIKNNQFEVGNFKVYTHLLNHPVTCYGY
                     KVLYKDKIFVYLGDNEPYYNVYKDNDPEVIEFAKQMNEQLIEFVKGADVLVSDAQYIP
                     AEYSKKTGWGHSTTHHVINLALKAKVKTLFLFHHEPLRKDREMDAIVTHYRNVLKKKE
                     LSLELFAAAERQSFTF"
     gene            complement(13672..14520)
                     /locus_tag="PLV81_11495"
     CDS             complement(13672..14520)
                     /locus_tag="PLV81_11495"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOM10763.1"
                     /translation="MAQNIVAKVKDSIEKMPPLSPVVHKIIIVANDVTSSAQHLTDVI
                     QLDPVLTAKVIRMVNSAYFGLPQEIKSLKQAVVMLGINTIKNVALSSALMGKIALKKG
                     ILDPEEFWKHSIGVAVASKLIAQRLGIEKKLLEEFFIAGLIHDIGKVLMNNFFPDEMK
                     QIIDTSQSQGGLIIDIEKNLLGLTHEEIGIAIGKKWNFENNLLYAVGRHHIPVLKGDS
                     AIYSMVVHVADTFAKVMKVGFSGNYVIDAIDEAIWQTLGISEEIVFEALAPLNDEIQK
                     AKLFLQ"
     gene            complement(14653..>14925)
                     /locus_tag="PLV81_11500"
     CDS             complement(14653..>14925)
                     /locus_tag="PLV81_11500"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOM10764.1"
                     /translation="IEVPAGTEKVSKSKPFVIASVYEGGTGYQAGLKEGDIIISVDGV
                     PVEGLQYDYIVKNLLRGKVGSVVTLEIQRGDNLMLFRVMRGKIVLK"
     assembly_gap    14926..15025
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15142..16227)
                     /locus_tag="PLV81_11505"
     CDS             complement(15142..16227)
                     /locus_tag="PLV81_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10765.1"
                     /translation="MKRFFILFSIFIFICSISANSFADQLTADRAIQENKEFIEFMDV
                     PISNFGEKYKETFKDIYQIHFNAEVAYLQSDYNRAFRNVYESQKKQADLYSQVVREYL
                     EETKKFLDAISPEVIKSKNKKARLYLNLAYRDREVARTHHTVADASNPKLCSNKIYKY
                     IEAIKVLRRARRYGFLAMFESQTDETKKKIYNHLFEIEREKGNVFYVRFISKTEQEII
                     DEMQKEYYDVQIVAMNAQPSKQEETYEKRIEKRARFRDERRVAEYLLTAEFEKAEPII
                     RDYVKDFNFNLIVATIEFLVASKSSMVGSFSVEQLKRYHDDNYSRYYSPSTLNAFAGE
                     MKVVDAIEKKPEGELPEEKPQTDNKQK"
     gene            complement(16245..>16430)
                     /locus_tag="PLV81_11510"
     CDS             complement(16245..>16430)
                     /locus_tag="PLV81_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10766.1"
                     /translation="IDYYENNSFTQYKQITFEVDKNFKYLSTYIPQEYFKRNGKYQLK
                     VLLDGKLMGTREFEVEK"
BASE COUNT         5147 a         3287 c         2693 g         5203 t
ORIGIN      
        1 ataattataa aaatattctt gattatataa cttactttgt aataattgtc atatgaataa
       61 tgtttgaata tcatgcgcaa agtccattat atatgtatca taataatgtt atggctcccc
      121 tgcagggtta atgcagggta ttatccattt atacttacca caacgcctgt tacctgccat
      181 cagtgtaaac attttgacaa ttcactctat cagtcacttg tttatacatt gacagcatca
      241 ggcattttta ttggcgatag ttcatcatat tcaatccctg ccggcgcata taacccacct
      301 ggtaacaatc aactgttgcg caatctttct atagatataa cctattcccg taagatacat
      361 tacaaagagc tcacaacata ccttaaagaa aaagtagaaa ctgaaagtta cacaattgcc
      421 ataaccattg catcatctga aaatccacag gaatttacag ccctttatcc caacatttca
      481 ccgcatcaac tggaacacac atttaattct attgctcagg atatactaca gttttattca
      541 tcaaaacgta ttcctgtact ctatcaaagc ccgtttgctt tttatgtcac cggattaacc
      601 ctggcacctg tttacagcat cctgcatgat aaattaaaca attacgcaca ctctgcaacc
      661 gggtttcagt gtttattcac aattaataac acatacatcc ctctaactat aatgccttct
      721 attacctggt ttacattaaa caatcccatt aaaaccatta atagctggca tagcgtttct
      781 tttatgatgc atgccggtta ccagcaagct ttaccattct cagtgagcat taccccctac
      841 attggagcag gttatatttt gcattacata gaaggcgata aaactcatac ctatccgcca
      901 tattcatact cacattcatt ctattataat cctcaggtag caacaggcat tatttttgaa
      961 cttattcttg atccacaact atcgctagaa gcaggtgctg catacaatat gttctttgcc
     1021 tcctcatcac agttttctta tcttcaatac tcttttggtt ttacattcca tggcaatata
     1081 ttactgtatc aggcaactcc ataccattaa ctttgtattt acttcttttg tttaaaattt
     1141 ttcctgtgtt ttaaatgtaa tgattcaacc attttagtaa aggtatcaaa cacatcctta
     1201 aactcatcgc ctttacgtaa ggggcgtatt tcaggtactt tcccttcaat aatttcattc
     1261 atatatcgtt ttaataaaaa catgggacca gctaccctgt gtgtgcgcct tacaacaata
     1321 ataaacgtaa caacaacaag tgttattccc agtacaccat taatccacat aaggtatcta
     1381 ttaatggcaa tactccttgt taacagttca ttgctttctt tcaatgcact ggcatttttt
     1441 tgaagatccc ctatagcttt gtaggcctca aaaattaaat tttttctatt gccatattgt
     1501 gataacattt caatggactt caagatatca tcctggacat tctgcaaatc cagctgatga
     1561 gccattgccc gtgaaataga gcggttgttc cacattacaa aactacctgt gataataaaa
     1621 agaatactga tacacagggc aaacaaacct gctacaccaa atcctgtttt caattgataa
     1681 tatctgttta tgattatgct tcttttaact ttcccatcca taagcttccc agtaaaaacc
     1741 taattctttt gataattgaa ttgcacggca tactgctctt ttgtattcat tgaccatcct
     1801 gatacattta ccggtattaa tgaaataaag taatgcctga atacatttgt ctgtatatat
     1861 tgctgcaaag taatggtgcc accaggacct gatctatccg attcgttgag tatgtttcca
     1921 ttgtcatcgg caattcgcac tatatattca cagttttccg gaacattatt cacagcaact
     1981 gttaaaaggc cattaacagc gctatttgca ggtgagctca ccagagcaag cgcattctga
     2041 ttccatgcat acgtaacaaa gttccaccta tgcataccag catcattccg tacataccac
     2101 gtttgaccct gatcaactga ttcaaaaata gtattatcag caatagcaat gattctgatg
     2161 ctatccggtg atatggatac acttttccag tttctgcgtc ccgcctgggt taaacaagtc
     2221 catacccgta caccatttac tacctttgat aaatatatat aaccagggtt ctgtgctgct
     2281 acaataataa aatcattgcc aatactacat acagcaatgc taatccagtt tgcagataaa
     2341 ttataaatat cagatgctac ccaacttcca ctttggtttg taataatata tattccatca
     2401 taacttgttg cagcaatctt cgtgccatca tacgctatag ccacacccga ccaattcttt
     2461 tgaccgggat ttgctatgct tgtccagcta tttccgccgc tggtagaaat atatatgtaa
     2521 tcattatatg ccgcagcaac catttttgac ccatcctgtg agcatgctac acttatccag
     2581 tttttagggc ctatgctacg ttgcgtccag gttgtaccac catcacctga agtatagata
     2641 taaccaccat aaaccgttgc agctaacctt gttccatcct gtgaacctgt tatattcgtc
     2701 cagctttttg gtgtattacg ctgtacccag gttgtaccac catcacctga agtataaata
     2761 taaccatcat aaactgctgc agccaacttt gctccatcct gcgatgcggt aatagattgc
     2821 cacctgtcat tgttactgtt gatagtattt acagtattat cagttaacct gtatatccca
     2881 ccccggtaat taaaatctat tgaatagata tcagcatcat aaggatcatg aaaataattt
     2941 tggcagttta tggttatact atctgtattc ccatccacaa gcatgactgt atagccatcc
     3001 ggttcgttgt tgaatagttc attgggatct atatacaatc caatatgttt atcctgtaaa
     3061 aattcatccc agctataatc tcttacaaaa tccattgaat ataaattgga tatataccag
     3121 ctttcatgtg tttttgttgt aaacattatg gaaagacccg aatggatatt tgctaacggc
     3181 ataacctttt taagcacaca gtttgcaaaa ttacctgcaa cagtttgcaa ctggacattg
     3241 tctgttgact gtaaaaattc taccaggtca gcataccatg gaatggaata gtaaaaatgt
     3301 gcagcaaagc gggcagcatc tattatattt gggcttgtat catatatatt ctggcattca
     3361 gaaataatag cattcatatt gctgattgct gattctattt gacgttcatc tattagcgca
     3421 agtgagcgcg tatctacttc ccctgcacta acagggtagg ctgtggtgct gcaaatgtct
     3481 tgagctattg ccagtgtcat gttgtcaggt ggcagctctg cggtaatacg tgtatagact
     3541 gcattgagaa gcgccatata attaaatcca cctgaaagca cctctccctg cgatgcaatg
     3601 atgtagtcaa ttcctgtttt actattacgt aattcatacg caacctctac tgtgcttttt
     3661 ataggtgcat ccaacacaag tactgaaata ttattatttc ttactgcatc tgcaaaatca
     3721 tgaatcgata aacgcgcatt tgatgtttca tcttctgcaa tgcaccatat gggttttgtc
     3781 tttcggtcaa gaagcacaaa aataatctgt tgtggtagat agttactgtt aacaaaggag
     3841 agtagctgac taacagtttg tgcctgtgct gtatttaatt cattacccac aaatcctgta
     3901 gtttgtaacg atatatcacg cctgtttcca tatatgcctt caaacagctg tgtgttagta
     3961 tcatacggat aatcagcaat gcccactatt cgcataactg atggatcaat gctacctgat
     4021 gcctgtatca tttcactgat atcgccagct atgtaatcac ctccagcata atcagcaggc
     4081 atatatacca gcacaacagt atctgttggt gcaggattgg tagaatatgt gtatgaatac
     4141 tgttgtatta atgggttgcc atggatatcg gttgcacctg ttgtaatctc cactatatac
     4201 tggtaaaagc ctgtaaggta tctgtcaggg gtgacagtgc aacaatatgt ttgctcgtca
     4261 taatcaattg ttaccgggaa gtaattacca taactatcgc acaacaaaac actactatag
     4321 agcgttcctt catccattgg ctcactgaat tggatctgaa ataccgtttc cacaccaatt
     4381 gggcttgcag gtgttatgct gataatttca ggctgaatat tctcctgcat agttctgaat
     4441 gtactggtgt aattgcgatc aagcggtatc ccattaatat cccgtatatc ggtagtaaca
     4501 gttattatgt aatccgtgaa aaattctaaa tgatcaggaa taaatgttac cgtagaggtt
     4561 tcactgttgt attgtatggt tccaggaaca gtagcatagc tatcttctat ttttattccc
     4621 tgtgacaatg tactttccat tacaggctca ctaaacacaa tccgtatata ctctccaata
     4681 gatacattgg tttgtccatc tgcagggtta atggaaagca cctgtggagg aataacctgt
     4741 actgtaaatt gccaggtata tggattttct aatgggttcc ctgcctcatc atgcagtgca
     4801 tcggttaccg ataccagata aacctgacct tctatcaatg attggatggg agtacatcta
     4861 atttctttat tttccgctat ataggtatat gtatactcaa tgttttgacc gctactatct
     4921 tttataataa caccctgcgc aatacttgaa atatctatgt gcccattatc aatgatcgtg
     4981 aatgatataa tagcattctt actcacatta tttcccacgg gttgcaccga tgatacatca
     5041 acacggggtt tctgtgtatc ataaaatgga gcaatagcaa acgaccgcta tactcctgct
     5101 gcatgtgtaa acccagcgta ttatatatac ccgttgtaac atatacggta tatgtgctac
     5161 cccattcaaa tccatgaact ggaataaaac gtaacgtttt agatgcaggg atatattcat
     5221 acgttccatt tacacttaag cttctttcat caagaacata aaatgtagaa gcatttacag
     5281 tactcatatc aatttcatga gaaaatgtaa ctgaaacagt actatcagca aataccactt
     5341 cattattaag cggtgtgtgg gatataatag taagataatc ctgcatacca tcaggattta
     5401 ttggttgatg tgatgaatta ttgtttttgc accctggtaa aacaattatt gtgagggtaa
     5461 tccaaagaaa tataataaaa tgataaagta gttttgagct tttgaaaatc tgatctatca
     5521 tatcacagat tataaaaatt tttgtatata tagtattcat caacagaaaa ttatttattt
     5581 ataaatgtca acatatatat taaaattatc ttttaatttt taataaatac atacgtatta
     5641 tttcactaat gtgatattga tttcatacaa atgaaatata aaaattatag gtatttattc
     5701 ttaatttcaa tttagttaaa ataatctggg aattacttat actctgatcg gttgtgtcac
     5761 ctatctattt aattctattt gacttttttt ataaaattcc aaacactagc agtaaacata
     5821 aatatctatt tccctaagga gtatatctat gagtgaaatt aaagttggcg aaaaaataaa
     5881 agaatttcgt gaggcaaaag gcatgtcatt acatgaattg gctctaaaaa cgggtttttc
     5941 ttctgcattt atatcacaaa ttgaaaatca tcttatttca ccaccccttg gagcacttat
     6001 aaaaatatca catgccctta atctagagat gggaagattc tttgggcaaa caacagaagc
     6061 accatttacc attgttcgca gtagtgaacg tcagctaaca tctcgtgttg catcaaaaga
     6121 aggtgtacgt tatggatata cgtatgaatc ccttgcacca gaaaaggtta atcgccacat
     6181 ggagcccttt attgtaacac ttgaacctgt tactggtggg cagccctaca gtcacgaagg
     6241 cgaagaattc atctatgtac ttgaaggcca ggtaaaaata cagctggctg attactctga
     6301 cattttatac cctggtgact gtatttatta tgattcaata gtaccacata gagtggatac
     6361 cgcaactgat tcaccagcaa agattcttgc tgtcatatat agttaacaat gcaattgaaa
     6421 atagtgtgga ataatactga tgcatttatc ttccaatgaa atattaacat tactgctttc
     6481 aatcagtata atgctgatta ttgcaaaaat tttcagtgag ctatttatac gtttcaaaat
     6541 gcctgctgtt ataggacaga ttattgcagg aattttgctt ggaccaacat gtacaggcag
     6601 catagcacct aattttttta catggctgtt tccttcaacc ggacttttaa agggcattat
     6661 tttcttaggc ttaatattta tcatggtaaa agttggcatt gaagtggaac tgtcatcaat
     6721 ttacaaacag ggtagatcaa ttactgccgt tagttttttt tcaatactta tcccgtttgt
     6781 tgcaggtttt attgcatcgt ggtacattcc atctttttca ggcacttata aatcgttacc
     6841 gtcgtcaatt ttctttgcta ttgtcacatc tattactgca cttccggtca ttgccaaaat
     6901 tctactggat gtcaaaattt ttcattcaga cctgggaatg attattttaa cctcagcaat
     6961 ggttaacgat attaccggct ggattttatt ttcatcatta attaaaacag ttgaaactgg
     7021 ttctattgca ataatctcaa ttattacatc gttgggatta acactattga taacaattat
     7081 cattttaaca attttccgct atgtccttaa tgaactgctt cctatagttc aggcacacac
     7141 tgaatggcca ggtggagtca taactctggg attagtatta tcaatgtttc tggcatcatt
     7201 aaccgaagcc attggtattc atgctttcct gggtgcattc atgggtggca ttatattggg
     7261 tgattccccc cacctcaacg cacataccaa agagattatc aatcaattca ttgataactt
     7321 tttttcaccg cttttctttg tctcaattgg tttgggcatt aatcttatca cccagctatc
     7381 acttatacag gcattcatga ttacactggt tgtatttatt tcaaatataa tagcaagcct
     7441 gattgccggg aaaatcagca acaatacatt catagattct ttagctatag gcgttggaat
     7501 gagtacaagc ggtacaatgg gtattattgt tggaatattt gcactgcagt atggaattat
     7561 taatacaaca acgtattctt ccatcgttgt tatggctatt gcaacctcac tgcttgcagg
     7621 accattgtta aaattaatac tcaaacccaa agaagaggtt tccctgattg aacttttaga
     7681 caacaatttt attcctgagt gcacatgcac caatcgcaat gaactgatac aacaggtatc
     7741 acagcacata caaaagcggt atggatacga tgcaaacaag gttgcacatg caataattga
     7801 acgggaacaa ctgatgagta ccggcattgg tcagtatgtt gccatacctc atgcccgcat
     7861 acctgagtgc aaaaaaccag tcgttatatg cgcaacacat ccacaggggc tggattttga
     7921 tgcccctgat ggcaaacctg caaaagtaat ttttatgata ataacacctc aacatgataa
     7981 tgatgtgcag attttcataa tatcaaagat agcacagcta tgttcaaatc ctaatgttgt
     8041 gttacaaata ctgaatgcaa aaaataataa tgaatttgtt gcaattctca aaacaacatc
     8101 tcacattatg gaaatggagg gttatggaac tgcatgacat aattaaaaac gatgacgaca
     8161 ggttatttat catttcacct gaatgcttta ttgtatttac gggtgattcc acagaagatg
     8221 aaaagccatt catacgtatt ggtaacagcc tgctactaac accgcgagtc atccccctca
     8281 ttgaaaatat cattattact gatcttataa ctggaaatcc ggcctgtgaa cagtataaca
     8341 ttgatccccg ttatctttcg tcaaaccggt atattggtag caagcttatt gtgaagcgct
     8401 atttagaatt tcaaaagctt tttggtcttg acctcaataa cgcaaccatt gtcgacattg
     8461 agcaggacat accacaactt tccaaagaac agattatctc cgacagagaa acattcttag
     8521 gggtttttta taccgatagt aactttaaga ttctccacaa tcagaaaaca atgtttgact
     8581 taaaggaaat acaccagaaa tatcctggcg atgtactggt ccactccaaa ctatcagaat
     8641 attcaaacaa atcacggtat gctggttgcg gttttgttat aactcgtgga gctacaatct
     8701 tttataaaaa taattctttt agtactgttg gtattccatc gcgctattac agtacatttg
     8761 cacaactcca ggttgaccct gcaaatattc gcgatgttat aatcacaaac accaacagca
     8821 tgccacttgt tccactgata aaatggaaaa atgcagctgg agggagactc aggatttttt
     8881 atgacaatga cgatgaaatc aaacttttac aaaagctttt taaccagtgc acgttgcatc
     8941 ataaatcatc aaaaaatttt gtgtgcgata atcccgaagg cataaccata cagaacattg
     9001 catcatcaca taactgcatc attaccataa aaaatgtaaa acctgccaca aaagatatta
     9061 ccattgtcta tatacatgac ccatcaggca tcactcaggc catagagaca gctgctaatc
     9121 tgtatattat tgactatgag atttataaaa aagcagcaat gctttgtgca agcctttcac
     9181 ctgttattat tgtcgatagt aaccgtcagg gagttcccat taaagatgtt acctactgca
     9241 ttccttcgca tcaatatgat gtacggtatt atcttgatga aaagaaactg ttatctgaca
     9301 tgctttcttg ttgcagcaag gaatttgcac aagctatttc cgaagaagat tttgatgaaa
     9361 tagaaaaact tcttacacag gaactatcgc cccatatagt gtacaactgc atccaaacac
     9421 tgcgtgttat actccactca acaactaacc gcgacctgta caaacgtatt gaaaaaatac
     9481 tgtataaaat gcaaacacgc ccattgcccg atagttaccg gtacacgatt atgctgcata
     9541 aatcatatgc atatatgacg tgccaaccag tgcaggtacc tcaggaatat ccatttgaag
     9601 ccattgaaca attagatgag ccatcacgac ctgcaccaca tacactgcct gatatatgta
     9661 atcgtattat tgaagaccgc aaacgattag aaatgctatt agacctgttc tatgcaaaca
     9721 acacagaaat tgcaaaagaa gctaaaagca ttgaaaaagc aataaacaaa cggaaaaaag
     9781 aaattacgca aacaccaaag ctagatgtat cactcattac taaagagaag ttgaaaaagc
     9841 gtttaactgt actgaagaaa gctgggatta ttgtagctgg catagctgta gctatactca
     9901 ttatggtggg aagctaccat gcgtttatac tgtatcagga aaagcaggag cgcgagcgtc
     9961 aggcccgcta tattgaatac ctcagtaaaa aatatgctat ccatatctct gatagggata
    10021 tctttttcta tgccaatgat gttgcagtaa agaatggcta cagtcgtata gatattaaaa
    10081 gccttaaaga aaagaatcca cactggattt accctggcaa cagatttatt cttttagatg
    10141 gggaagttgt catcgttaaa ccaggtgata ctctgtgggg aatatcagaa agaaaattac
    10201 tttcactcaa tattaccttt tataccttac tggatgaatt agagaaaaaa attgataaag
    10261 gtattattga tgagcatctg gcacaaaaag ccatgaatgc agcaatacat actaaacaca
    10321 gagaaagggt taaagcatta ctacagaaga tgaatgaatt aaaagctaaa caaaacaata
    10381 cttaaggtca tggttacact gcatggaagt taaagaccct ataaactatt acgtagagcc
    10441 ggttgaaata gaaatatacc tgaaaaaagc aggtaaagtc cgcactatca taaaagacat
    10501 gtatattgaa cttattgatc cggaaccgct ggataatgat accagtaaaa agatttttga
    10561 ttattttaaa agccgcaatg aacccattga cgtaatagaa attaccaaca ttttcccgga
    10621 attgatatct atagtttttg aatcatacta tcacaatatt aatttatatg aaaagctgag
    10681 catgtatttt aaagcaggtc tttctggtag cgccgattca tggcgacttg cattatactt
    10741 tacagagcta ttaatgaaat ttgagccaac tatagcatcg tcgcagcata taggtgattt
    10801 tcagacatat aatcttaatt attgtatccg caaactcaat gcattgggtg aaaaattttt
    10861 acttgaagat agtaccgtta tgtaccttat caaacggcgg aacaaagcct atgaaggaaa
    10921 acctaaagac aaggaatttg aaaaactggt tgagctatgg caatttaatg tcaaagaacg
    10981 tcccttttaa aacgtatttg acatttattt caaaatcatt tactatagaa gcatatcgat
    11041 aacgtgaatg ttgcgagtga tgtttttatg aaaaatccct tttacattat ctacgaccca
    11101 gcatatacaa ccagctatgt aactgcttca gtggaaaaac cggaacgcgt ccgacaaata
    11161 atgaaggaat tgggcgatag ttaccccata ataaaaccca caagctgcac tgatgaggat
    11221 atacttctct gccattctga aggcttgctt accatggaaa aacaggatga cgtgcgcttt
    11281 acagtagcaa agcttgctgc aggtggtgca attaccgcag ctaccatggc aatggaaggc
    11341 aaacccagct ttgctgttat acggccaccc ggacatcatg ccaaccctga tcataactgg
    11401 ggattctgtt attttaacaa tatgggtatt gctataaaac gcctgttagc tgattctatc
    11461 attcaaaaag ccattattct tgatattgac ctgcattttg gtgatggtac tgataccatt
    11521 ttcaaatcca ctattaatgt tcctgtgtta aatatacagt catcaaaccc tgttgatttt
    11581 atcaatgaaa caaaaacacg actgcagcag tgtggcagtg ctgatatact gggtatcagt
    11641 gcaggatttg accagtatat aaaagattgg ggagcaaacc tttcaacgca ggactatttt
    11701 accattggcc agatagctgg taatcatgca agacattact gcaaaggcca tatatttgcc
    11761 attttagaag gtggttacta tatccctgat ttaggaaaaa atgttatagc tcttattgaa
    11821 ggaatttgta atggccttta tgactgataa ggctttataa agtatgcacc ataaaattac
    11881 aatttcgctt tttattaaat tttttctgta taataccttt gtatcaggaa ctatctgcct
    11941 atttcttttt ataaataaag ctattagttt taccattttc actgtgataa gcttctttgt
    12001 gataaatatt gttgccgcat gtattgcggt ttcatttatt gcaaaaccct ttacaatgct
    12061 tttgcgttca atccagtcac tggttaatga caggtacaca tcttccaacc cttgctcaaa
    12121 agaacatctg atcatgctga cacaagaact tgaacaattt aaagcaaggt ccgattcatt
    12181 gttgcgtgaa aaggacacca tagttaatga actgattgaa tccaaaaagc tattagaaaa
    12241 atattccaat aaccttgaag aaatggttga acagcgcacc gaaatattaa agtggctttc
    12301 aattactgac ccgctaacag ggctttacaa tagaagatat ttcatagaac agattgaact
    12361 tgaatttaaa cgttccaaac gttacaaccg ccacttatcg cttttgatgc ttgatattga
    12421 tcactttaaa tcagtaaacg ataattacgg ccatcaggtt ggtgatattg ttttacggaa
    12481 gatatcgtca ataataataa gccagcttcg tgattcagac cttgcctttc ggtatggtgg
    12541 tgaagaattt atggtaatac tgcctgagac aaaagcagat gatgctatca atgttgcaaa
    12601 acggatgaaa caggaaataa tggaaacgga acacatctac cgtaatctta attttaaagt
    12661 aacagcaagc ataggcattg tatcaatcaa ggatatgctg ggcaaatttg aaacggttga
    12721 tgatattatc aaaaaggtgg atgataatct ctataaggca aaaaattccg gcaggaatac
    12781 cattatatat gaatgattaa aatgtaaagg actgcctttc agctgctgca aacagttcaa
    12841 gcgatagctc ttttttcttc agcacattcc tataatgagt aactattgca tccatttccc
    12901 tgtctttgcg taaaggctca tgatgaaata aaaataatgt tttaactttt gccttaagtg
    12961 ccagattaat aacatgatgc gttgtactgt ggccccaacc ggttttcttt gaatactcag
    13021 caggaatata ctgtgcatct gatactaata catctgcacc ttttacaaat tcaataagct
    13081 gctcattcat ctgttttgca aattcaatta cttccgggtc attatcctta tacacattat
    13141 aatatggctc attgtcgccc agataaacaa aaatcttatc tttatatagt actttatatc
    13201 cataacaggt taccggatga ttgagaaggt gcgtgtaaac cttaaagtta ccgacttcaa
    13261 actggttatt ttttatttca taaaaatgaa tactggcgcg taattcatcg gtgcggaccg
    13321 ggaaataact gtactgcatc tgcccgcgta taatttcctc caggctcttt tcaaatgttc
    13381 caggaccata gatattaacc gtatttcctg gtatgtagat aacagggaaa aaaggaaaac
    13441 cattaatgtg gtcccagtga ggatgagaaa tgcataaatt gacagtatag ttttttgaca
    13501 gatccagtga ttcgcccagt accctaagcc ctgtccctgc atcaaatact atccagtcac
    13561 ctgcatctga tcgtaaatcc aggcaggcag tattccctcc atatttcata gtatcagggc
    13621 cgggaacagg tatagaacca cgcacacccc agaatgttac ggtcagttta ctcattgcaa
    13681 aaataattta gccttttgta tttcatcatt cagaggagct aatgcctcaa aaactatctc
    13741 ctcggatatc ccaagcgttt gccatatagc ttcatcaata gcatcaataa cataattccc
    13801 actgaatcca actttcatga ccttggcaaa cgtatctgct acatgaacaa ccatggaata
    13861 gatggccgaa tcacctttta aaaccggtat atggtgcctt ccaactgcat acagaagatt
    13921 attttcaaaa ttccatttct tccctattgc aatgcctatc tcttcatgtg ttaaacccag
    13981 aagatttttt tcaatatcaa taatcaatcc gccctgtgac tgcgacgtat caattatctg
    14041 tttcatttca tccggaaaaa aattattcat taaaactttg cctatatcat ggataagccc
    14101 ggcaataaaa aattcctcca gaagtttttt ttcaataccc aaccgctgtg caataagttt
    14161 ggatgcaacc gccaccccaa tagaatgttt ccagaattct tcagggtcca gtattccctt
    14221 cttcaatgca atttttccca ttaaggcaga tgataacgca acattcttta tagtgttgat
    14281 gcccagcata accactgcct gctttaatga cttaatctcc tgtggaagac caaaatatgc
    14341 agagttaacc atacgtataa cttttgcagt taataccggg tcaagctgga ttacatccgt
    14401 caaatgctgt gctgatgagg tcacatcatt tgccacaatt attattttat gcaccaccgg
    14461 agataatgga ggcatttttt ctattgagtc tttaaccttg gctacaatat tttgagccat
    14521 caacccacct gctattacta tctattattt ctttcagaag aaaaaccagt atatactatc
    14581 tgcaataata acaacccaga aaagaactga aaaaaccagt atgatactat ctataacttt
    14641 cttcatggta ttttatttca aaacaatttt cccacgcata actctaaaaa gcatcaagtt
    14701 gtctcctctc tggatttcca gtgttaccac actcccaacc ttgccacgca ataaattttt
    14761 tacaatataa tcatattgca aaccctcaac cggaacacca tcaacggaaa taataatgtc
    14821 accttcttta agccccgcct ggtatccggt tccaccttca tacacactgg ctataacaaa
    14881 tggtttactt ttactgacct tttcagtacc ggcaggcact tcaatnnnnn nnnnnnnnnn
    14941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15001 nnnnnnnnnn nnnnnnnnnn nnnnnagtaa ttcattcatc attcttctgt gtattgaatt
    15061 gagcaactca gaattatcca tataaagcca tcctgtacat taatgatttg attctgccct
    15121 gagcctgctt taaatagtat gctacttctg cttgttatct gtttggggct tttcctcagg
    15181 caattcccct tcaggctttt tttctatagc gtcaacaact ttcatttcac cggcaaaagc
    15241 gttaagtgtt gaagggctat aataacggga gtaattatcg tcatgatatc gctttaattg
    15301 ctccactgaa aagctaccta ccatggatga tttactggca actaaaaatt ctatagtggc
    15361 cactatgaga ttaaaattaa aatctttcac ataatcacga attattggtt cagctttctc
    15421 aaattctgca gttaaaagat attccgcaac ccttcgttca tccctgaaac gcgctcgttt
    15481 ttctatacgc ttttcgtacg tttcttcctg ttttgatggc tgtgcattca tagccactat
    15541 ctgaacatca taatattctt tttgcatctc gtcaattatt tcctgctctg tttttgaaat
    15601 aaatctgaca tagaatacat tccccttctc gcgctctatc tcaaaaaggt gattatagat
    15661 ttttttcttt gtttcatcag tttgagattc aaacatggct aaaaaaccat acctgcgggc
    15721 acggcggagc acctttatgg cttcaatata tttatatatt ttatttgaac ataatttagg
    15781 gtttgaagca tcggcaacag tatgatgggt ccgtgcaacc tccctgtctc tgtatgctaa
    15841 gttaagataa agccttgctt ttttattttt ggatttaata acctcagggg atattgcatc
    15901 caaaaacttc tttgtttctt ctaaatattc acgcactacc tgtgaataca ggtcagcctg
    15961 ctttttttgt gattcataaa cattgcggaa agctctattg taatctgatt gcaaatatgc
    16021 cacttctgca ttaaaatgta tctgatagat gtctttaaat gtttctttat acttctcacc
    16081 aaaatttgat attggcacat ccataaattc aataaattct ttattttcct ggatagccct
    16141 gtcagcggtc aactggtcag caaagctgtt tgcagaaatt gaacatataa agataaaaat
    16201 actaaacaag ataaaaaatc gcttcattgc acaccttcat atatttattt ttctacttca
    16261 aattctcttg tccccataag tttgccatcc agcaacactt ttaactgata tttcccattt
    16321 cttttaaaat attcctgagg tatatatgtg cttaaatatt taaagttttt atctacttca
    16381 aaagtaatct gcttatactg tgtaaaagaa ttgttttcat aataatctat
//
LOCUS       DAORYL010000068        16408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_2875_length_16408_cov_12.192381, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000068 DAORYL010000000
VERSION     DAORYL010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16408
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2875_length_16408_cov_12.192381"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1341)
                     /locus_tag="PLV81_11515"
     CDS             complement(112..1341)
                     /locus_tag="PLV81_11515"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HOM10767.1"
                     /translation="MENKNIFFAMIIICVVALIDSLYLLYVHYFPLVPDAPLFALCTA
                     GTMFDCNAVNTSAYAVLFGLPLSAWGLSFYIFFITILMVYYTYSRHFIIEFSMLWLTL
                     FAVLLSVVLGFISFSKIKKFCSFCMILWLCNGMLLVLVCVHITRLYNGLKSAVNTIHD
                     FDVIALIKDSHVQKIILILSIAGIISLGLGYGLDASLKYAYNQKEKERQAKLIEEFKK
                     DYEQYEKVSITVDDIEPFIGKKENPVHIVVFFDFNCGACHRAIGLLNELALKYEGSVA
                     LYLRHFPLDGTCNKFIEHTKDGASCHASLVACALYGEKEYHEYIMQLMTHRGRVEDDV
                     IKEVVTGLKKNYTQLEAKANTPHVNKLLQNDITLGGKLGIHATPTIIINNTKLRPGIP
                     PAYILEMAIKIEMAKKM"
     gene            complement(1382..2428)
                     /locus_tag="PLV81_11520"
     CDS             complement(1382..2428)
                     /locus_tag="PLV81_11520"
                     /inference="COORDINATES: protein motif:HMM:NF025182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HOM10768.1"
                     /translation="MVKKYCTWCTLGTVILSIFCYTQCSKTSSVTEYGVFAPSRLQSV
                     KGQDGCTPVDFDERYTLWTFGDTIIDEGMISNSLAFTQKINAANVTQLQFEYYREHGK
                     IAQFIKNTSDENPSKDRLWAFDGIRIGNTVYVYYAHVYIHDPAKPLSFTMKESSIAYW
                     DVPAQWEIGKPIRFTRKKNMFLGNVPALGASMLLHNDFVYVVGHISEENRSSLIIGRV
                     HKNHILKQHQYEFLQGDSNWDNKLNSAKRFYGDVAGECSLTYDGITRSYSIVYCQLFT
                     GNIVMCKAPTIEALPQAKKEIVYTPPELHGTNMLYYSAKTILQEGKQWYIVYMNPLEY
                     QPYCIKVDVGTRCW"
     gene            complement(2453..3484)
                     /locus_tag="PLV81_11525"
     CDS             complement(2453..3484)
                     /locus_tag="PLV81_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10769.1"
                     /translation="MKKMYYVLGAIAALTLLYFAFFHKSDTVQSRIDYIKNGGVVPPV
                     NMLLQAFQADEELRARLQADFDMQDVVQYFREKYGKTIHHKHTQVMLVEKLMGYLMKM
                     YPDDWVVYMYAMLREIFPGMENEIFDTFEKLHKYNTWLEENEHDLAQLNSKDKRNALW
                     EKRYEIFGQDADQVWEAEKRIEPIYNALDTIQESDMPFNEKLSLYKQSIQEAYKSNTD
                     TFVARKQQELMNSFLDVESVQKDLANMTPQERKENLKEVRKAMGLDSAALQRWEELDQ
                     VRDQRWDAGMQYMQQRAELEKKYTGAELEKQLDSLRMKYFGDEAETIKNEEESGYYRF
                     SQPRKYGLE"
     assembly_gap    3604..3703
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3783..4853)
                     /locus_tag="PLV81_11530"
     CDS             complement(3783..4853)
                     /locus_tag="PLV81_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HOM10770.1"
                     /translation="MFLTVCIQTSFAAVDKTTYPVIFAHGMGGFDTILGYNYWGDDFG
                     VFVGDPCDAFLEVTCNPNINKGQQSFVAQVQPLHNSEYRGAQLADQIEGYMATSGAKY
                     VNLVGHSQGGMDIRKAAKVLYQRKGYQVVKVLMSISSPHRGSPLGKYVLEMGTLGDII
                     KFIFNLYGDIVYKQGNDTVEALKQFVYDDYDPGDGKITGAKVFNQNNPVSSLYASRYV
                     SIMTAQKGLDLNPLLLIVKVLYDIDGNGYCVDDCDNDGVAGCGNGIKDDLDDDGMVGI
                     NAQQMGYRLKYHEVFLGQDYLTTDTSLGYVGNINYTNAAQMTSGLDLINQDHMDVIGL
                     GPDTFDEMEFYAAVFDYIAYYD"
     gene            complement(5055..>5531)
                     /locus_tag="PLV81_11535"
     CDS             complement(5055..>5531)
                     /locus_tag="PLV81_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HOM10771.1"
                     /translation="MKVFNNTYPVSSTYAARYVSIITAQTGVAVNPALFLLRQGFYNI
                     DGDGYCTDDCDNDGAAGKGDGTRTDLDDDGLVGINSQQMGYRLKLNEVFLGQDYLTTD
                     TGLGYVSSINYPNSAQMTSAADIVNQDHLDVIGVGPDMFDEMEFYAAVFDYIAYYD"
     assembly_gap    5532..5631
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5718..5984)
                     /locus_tag="PLV81_11540"
     CDS             complement(5718..5984)
                     /locus_tag="PLV81_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10772.1"
                     /translation="MRRHNGAIVISSAGGEEYAYESAQWKNGVFTFSVIEGLTMKKAD
                     RNKDGIITISELMSYVANRTRELTGGRQNPTSRKENVEMDFKIW"
     gene            6122..7243
                     /locus_tag="PLV81_11545"
     CDS             6122..7243
                     /locus_tag="PLV81_11545"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HOM10773.1"
                     /translation="MKNILFYSEDLAQVEYSPTHPFKPMRAKQMMDLLNRYGLLEDTQ
                     ILEHPFLEEELLYLFHTPDYIQALKTCEHGEFDINLLTYGLGTHDNPIFKGVYSFSLQ
                     ASGGTFLGAKKLVETDATFAFNPIGGFHHAMRDHAEGFCYINDLAIALHYLIKKGFKC
                     AYIDIDVHHGDGVQSAFYDDNRVLTISIHESGQFLFPGTGFENEIGEGPGYGYNVNIP
                     LLKDTDDEIYLYAFEEIVPPLLQSFKPDIVCIQVGGDSHKDDPLAHLNLTSNGYEKVL
                     TLIKQYSGKVLGTGGGGYNLYKTSALWTLAWSTFAGIKPVDNFMGVVGGMMFGPEMDA
                     GSLYDTPYHIGGLTKEQCFSYARDVVDYIKQTVFPIHKI"
     gene            complement(7313..7963)
                     /locus_tag="PLV81_11550"
     CDS             complement(7313..7963)
                     /locus_tag="PLV81_11550"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOM10774.1"
                     /translation="MLKICAISDIHRGYNNIYRLQPEIESSDIVVIAGDIGPLKWYTR
                     WEAYKGAIAQIHKPVLAVHGNWDGSRIQEFLAPYSIHAGFAIVDDIGFFGVGGSIKTP
                     LHTPVEYTEDEIYNFLCRGFEHIKNQPIKVLISHNPPKNVCDRTIFKTNAGSTAIAQF
                     CLDYNIDLCICGHIHEAHGVAQLGNTTVVNAGTFKSGYYASILVALNGVEAAIKRL"
     gene            complement(8087..8245)
                     /locus_tag="PLV81_11555"
     CDS             complement(8087..8245)
                     /locus_tag="PLV81_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10775.1"
                     /translation="MKNAQLYIYREYEEPFQCATLPQVDECDRLVAAIKYFGVLIPAE
                     IFDEDRGI"
     gene            8457..9086
                     /locus_tag="PLV81_11560"
     CDS             8457..9086
                     /locus_tag="PLV81_11560"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOM10776.1"
                     /translation="MVTQLTLLEFSIITLTVSLSGVMAPGPMSAVTINHGTRSRLAGL
                     MVATGHAIVELPVILALFFGIKLITQFTNITTYITLAGSIVLFILATLTLKSLRNNNT
                     SVQSYTPMVDGIVLSAFNPYFYVWWLTIGLSLIQKAFVFGTVGLIIFSVLHLACDYIW
                     LFVLSIISYAGASFIGKKFKIAVSIITVIMLYAFGIFFMYDALHAIVSQ"
     gene            9213..9563
                     /locus_tag="PLV81_11565"
     CDS             9213..9563
                     /locus_tag="PLV81_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10777.1"
                     /translation="MNNNTQNTIAQLKEELIPLIKTVVQDEVKSNIEDFMRQSELRAK
                     ELSLIERIIRVEEEIKALKEIEKARFEAMETRFEAMETRFEAIEKRFSSLQWFMGIGF
                     TIISILIAIFGLLK"
     gene            complement(9579..10661)
                     /locus_tag="PLV81_11570"
     CDS             complement(9579..10661)
                     /locus_tag="PLV81_11570"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOM10778.1"
                     /translation="MEDSAKRLEVIVEFMVPGATLSGDTFDSENNFLYPAYTVYTSEI
                     IDTLKAKGIKRIYYTPAENNDATAFTEQAQDIWESLFEAVRTNARPDLEQTKDFVLSL
                     HQYVKQNKDRFLYLMKIKDYDDYTFTHSVNVGILAMTMASKHGLEEKLVREIGLGAFL
                     HDVGKLMIPKEILNKKEKLTSDEFEIIKKHPEFGYEYVAKHDHIPPNALDVILHHHEN
                     YNGKGYPKGFQDEEIPIGARITSICDVYDALITPRPYKRAYSSREAILSIMKESRVKF
                     NPVLVSRFVRDMTPLLQKEPLLPVGTIVMLTTGEVAQVEELHSTGDLRPIVKILTNDK
                     QQKINRPFTVDLTLDSTRSVKRIIPR"
     gene            complement(10674..11081)
                     /locus_tag="PLV81_11575"
     CDS             complement(10674..11081)
                     /locus_tag="PLV81_11575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HOM10779.1"
                     /translation="MEKMVQDPIGYARDVVGKDPLATFLGIEVEDVKHGYARCGLTVK
                     PEYLNAVERAHGGIIHAVADQAFAVASNSMGTMAVALTMTLQYLQAALDGERIFAECM
                     LVHAGRKISSWRLEVRGNNDAIIAIGDAVAYHK"
     gene            complement(11124..12404)
                     /locus_tag="PLV81_11580"
     CDS             complement(11124..12404)
                     /locus_tag="PLV81_11580"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOM10780.1"
                     /translation="MKKSYLAFCDIEGNVYKHPGALAAFRSGRRFVKVDEKELIPIPD
                     GSTLYLLPQRYPVEGKNFKTITHYNGNTIWAVSSFLSSGYLRTYLPAYVKTKDAPVLS
                     LWAYCGLVFVGDDFYVPALRIDPDPRSDPALHRNDIELFKQIKVVKEQYPSNSLVRQL
                     AKCSTQYGCLCARNFFLSRHEAPLPTTKSCNALCLGCFSFQKDTGVHVSQYRLTRQPS
                     PQEIAEVMLHHIERVDRAVVSFGQGCEGEPLLRAKDLAAAISIVRSKTSKGTIHLNTN
                     GSLPQGLQQCIDAGLDSVRISMNSPTEEYYTRYYRPKNYSFKDMLRSIEVALKAGIFI
                     SINLFFLPGFTDAQSEVERLCTFLDNFPVNMIQTRNLNIDPDYYLDSIGFEDEEAIGV
                     RQLLKLLQTKYPRLTLGYYNPPKEEFGVITKVDW"
     gene            complement(12416..12928)
                     /locus_tag="PLV81_11585"
     CDS             complement(12416..12928)
                     /locus_tag="PLV81_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10781.1"
                     /translation="MIEKLRTALMESSLSHEELQHIMFLLFCCGDSNRQVPEDLIKAG
                     YISINDNCCTLTDKGRAYRDSLLQKLAKFHAASDEVFRQYFDNELLPIKKKMQLLKHE
                     WRSTRDKRLRYKETLMKQGKDKAAIKKDVQYKKLQRQQNTLRTRMRHAEKEYNKKLSA
                     CIKRIGILDL"
     gene            complement(12925..13122)
                     /gene="thiS"
                     /locus_tag="PLV81_11590"
     CDS             complement(12925..13122)
                     /gene="thiS"
                     /locus_tag="PLV81_11590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOM10782.1"
                     /translation="MRIRVNGIDIEFEKTTLKELLDLYRFNPSKIMVEKNGRIVEKEN
                     YATETLQDGDVLEIARFVGGG"
     gene            complement(13136..13858)
                     /locus_tag="PLV81_11595"
     CDS             complement(13136..13858)
                     /locus_tag="PLV81_11595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOM10783.1"
                     /translation="MAQFFVSAPGHDNTVVIEGKDYYHLVRVRRVRVGDSIDVIDTEG
                     VRYRATIAHIEAQKIIATIMQTITYPEISLNLTLCVALLKGKKFDLVIQKAVEIGVRS
                     IIPVVTERTIPDISEKEEKKLERWQKIATEAAKQCLRKDVPRVSSVMEYTDVLQLSKG
                     VGILAHADPEAQNLRQYLKDNPQHNDVMLLTGPEGGFSKKEVEIARQFGWIVLYNGAT
                     QLRAETAAIVLPAIIIYEWGYT"
     gene            complement(13860..14639)
                     /locus_tag="PLV81_11600"
     CDS             complement(13860..14639)
                     /locus_tag="PLV81_11600"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOM10784.1"
                     /translation="MKNIHSIMLVILLGVSIIFFSHDHSVAQADREIELTAQEILARV
                     DRIMEYPDGIIKGYMMHITPDGSSQKLQITGYINKNQFLFDIIKPARGSMQKILYRIG
                     GEDLWVYDMAARALFHKMGIDKYDDILATNFFYVDLSNADLQSNYVARIDGSVILKNQ
                     DCYKLTLDPIDKTGAYGKLTLYVIKQNYIPLRIDYHDQDKVIFKTLSIAKIGQKDGKS
                     FPIRYDMLNIKKGTMTVVEFTSIEDGATINTEIFRPEKLGD"
     gene            complement(14790..15194)
                     /gene="aroH"
                     /locus_tag="PLV81_11605"
     CDS             complement(14790..15194)
                     /gene="aroH"
                     /locus_tag="PLV81_11605"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOM10785.1"
                     /translation="MMVVRGVRGAITVDENSKEEIVHRTEELLRAIVTVNKIQTNDIA
                     SAIFTVTDDLNAEFPAVAARKLGWIHIPLLCSREIPVPGSLGMCVRVLLHINTDKNPE
                     EIVHVYLRDARKLRPDIGTPEQDKYYVSDSQT"
     gene            complement(15191..>16408)
                     /gene="aroA"
                     /locus_tag="PLV81_11610"
     CDS             complement(15191..>16408)
                     /gene="aroA"
                     /locus_tag="PLV81_11610"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOM10786.1"
                     /translation="GSKSHTIRALVCALLADGTSTIQMPLDSSDTRSCLSMIRQFGAT
                     VEEHEHEWVVKSNGAPQLPDDVIDVGNSGTSLYFGIGVASLIPGMTVFTGDHQIRNRP
                     AEDLLNAITELGGYAVSTRSNGKPPVVVQGPIIGGHVSIRAITSQYLSSLLVATPKAK
                     HNTIIDVPLLNEAPYVGMTLWWLKELGIEYHHTGYEQFIVKGGQSYKPFTKYIPADFS
                     SATFFMVAAAITQSSVVLKGLDFNDTQGDKEVVNILKAMGADVDIMPDAIAISGKPLK
                     GGVFDLNAMPDALPALSVCACFADGETRLVNVPQARVKETDRIAVMCAELKKMGADIE
                     ELPDGLIIRKSTLEGAHVNGHHDHRVVMSLAVAGLAAEGSTTIDTAESVSVTFPNFVE
                     LMQALGASIRKEE"
BASE COUNT         4803 a         3567 c         2722 g         5116 t
ORIGIN      
        1 caaattactt tttaaaacaa aaagtttaaa caatattaac cgtctaacta caatagggat
       61 ccgatttttt attagtcttt aattaaaatt ttaaaattct tgaccaaaat ttcacatttt
      121 ttttgccatc tctatcttta ttgccatttc cagtatatag gctggtggaa tgcctggtcg
      181 taattttgta ttgttaatga tgattgtggg ggttgcatga atgccaagtt taccacccaa
      241 tgtaatgtca ttttgcaaca gtttgttaac gtgtggggta tttgcttttg cttcaagctg
      301 tgtatagttt ttctttaaac cggtaacaac ttcttttatt acatcatctt caactctacc
      361 cctgtgggtc ataagctgca taatatactc gtggtattct ttttctccat acagtgcaca
      421 ggcaaccagt gaagcatggc atgaagcacc gtcttttgta tgctcaataa acttattgca
      481 ggttccatcc agcgggaaat gcctcaggta cagggcaaca ctgccttcat attttaacgc
      541 taattcatta agcaatccaa tagctctatg acaggctcca caattaaaat caaagaatac
      601 aacaatatgg acagggtttt ctttcttccc tataaaaggt tctatatcat ccactgtaat
      661 gcttactttt tcatattgct cataatcttt tttaaattct tctataagct ttgcctgtct
      721 ttctttttct ttttgattat atgcatactt cagtgacgca tccagtccat accctaaccc
      781 caatgagatt ataccagcta tggatagtat aagaataatt ttttgaacat gggaatcctt
      841 tatcaatgca atgacatcaa aatcgtgtat tgtgttaaca gcacttttaa ggccattgta
      901 caggcgggtt atatgcacac acaccagcac aagtaacata ccattgcata accacagtat
      961 catacagaaa gaacaaaatt ttttgatttt actaaatgat ataaaaccaa gcacaacaga
     1021 aagtagcact gcaaatagtg taagccacag catactaaat tctattataa aatgacgcga
     1081 gtatgtataa tacaccatca gtatggtaat aaaaaatata taaaacgaaa gcccccatgc
     1141 tgacaaagga aggccaaaaa gtactgcata agcactggta ttgacagcat tgcaatcaaa
     1201 catggtacca gcggtacata acgcaaacag aggtgcatct ggcaccagcg gaaagtaatg
     1261 gacatataat agatataacg aatcaattaa agcaacgaca cagataataa tcattgcaaa
     1321 aaatatattc ttattttcca tgaaacatcc ctcaaaatat ataatttcat aaaataatac
     1381 ttcaccagca tcttgtaccc acgtcaactt taatacagta cggctgatac tctaatggat
     1441 tcatatatac tatataccac tgctttcctt cttgtaagat agtttttgcc gaataataca
     1501 gcatatttgt cccatgcaac tctggcggcg tatatacaat ttcttttttt gcctgtggca
     1561 gtgcttctat cgttggtgct ttacacatca cgatattgcc agtaaaaagc tggcaataca
     1621 ctatactgta tgaccgcgtt ataccgtcat atgtcagaga acattcacca gcaacatcac
     1681 cgtaaaatct ttttgcagaa ttaagtttat tatcccagtt actatcgccc tgtaaaaatt
     1741 catattgatg ctgtttcagt atatggttct tatgtacccg tccaattata agtgaacttc
     1801 tgttttcttc actgatgtga cccactacgt aaacaaaatc attatggaga agcatcgatg
     1861 ctcccagggc cgggacattg cccaaaaaca tatttttttt gcgggtaaac cgtatgggtt
     1921 tcccaatttc ccattgagca ggaacatccc agtatgcaat tgaactttct ttcatggtaa
     1981 atgacaaagg tttagcagga tcatgtatat atacatgagc ataatacaca tacacagtat
     2041 taccaatacg aatcccatca aatgcccata atcggtcctt agatggattt tcatcagagg
     2101 tgttttttat aaattgtgct atctttccgt gttccctgta atattcaaat tgcaactggg
     2161 tcacattggc agcattaatt ttttgagtaa acgccaggct gttagaaatc ataccttcat
     2221 caataatggt atcgccaaat gtccacaagg tataacgctc gtcaaaatct actggcgtgc
     2281 agccatcctg cccctttaca gattgcaggc gagaaggtgc aaacacacca tattcagtaa
     2341 cactggaagt tttactgcat tgtgtataac aaaatatact gagtataaca gtacctaaag
     2401 tacaccatgt acaatatttt ttaaccaaaa aaatcaccat tgcaagctac tgttattcca
     2461 atccatattt acgtggctgc gaaaacctgt aatacccgct ttcttcttca ttctttattg
     2521 tttcggcttc atcgccaaaa tatttcatcc tgagactatc cagttgcttc tcaagctcag
     2581 cacctgtata ttttttttca agctcagcac gctgctgcat gtactgcatg cctgcatccc
     2641 agcgctgatc acgtacctgg tcaagttctt cccagcgttg cagtgcagcg ctgtcaagcc
     2701 ccatagcttt gcgtacctct ttcaggtttt cctttctttc ctgtggtgtc atatttgcca
     2761 gatctttctg tacgctctca acatcaagga atgaattcat caactcctgc tgcttgcgtg
     2821 ccacaaatgt atcagtgttg cttttatatg cttcctgaat gctttgttta tacagcgata
     2881 gtttttcatt aaagggcatg tcgctttcct gtatagtgtc cagggcatta taaattggct
     2941 caatcctttt ttctgcttcc catacctgat ctgcatcctg accaaatatt tcgtatcgtt
     3001 tttcccacag tgcattgcgt ttatcttttg aatttaattg tgccaggtca tgttcatttt
     3061 cttctaacca tgtattatat ttatgcaatt tttcaaatgt atcgaatatt tcattttcca
     3121 tccctggaaa tatttcacgc aacattgcat acatatatac tacccagtca tccggataca
     3181 ttttcataag gtaacccatg agtttttcca caagcattac ctgagtatgc ttgtggtgaa
     3241 ttgtttttcc atatttttcc ctgaaatact gcacaacatc ctgcatgtca aagtcggcct
     3301 gcaggcgtgc tcgcaattcc tcatcagcct gaaaagcctg caacaacata tttaccggtg
     3361 gcacaacgcc accatttttg atataatcaa tacgtgactg gacagtatca ctcttatgga
     3421 aaaatgcaaa atagagtaaa gtcagtgcag cgatagctcc cagcacatag tacatttttt
     3481 tcatagatat ccactcctcc tataaaatat tgaatcattt tgtcattgcg agtcccagta
     3541 taatcatcca tcctattctg tcatcctgag ccccgatgaa gcatcctatt ctgtcatcct
     3601 gagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttttaat atatatcatg
     3721 acataaaaaa atccttctcc agtagctgaa gaaggatttt tatgtataac ttgaaaatta
     3781 tatcaatcgt agtaagcaat ataatcaaat actgctgcat aaaactccat ttcatcaaat
     3841 gtatcaggac caagtccaat aacatccata tgatcctgat taattaaatc caaacctgaa
     3901 gtcatttgtg cagcattggt ataattgata ttgccaacat atcccaatga tgtatcagtt
     3961 gtaagatagt cctgccccaa aaaaacttca tgatatttta acctataccc catttgctga
     4021 gcattgatcc ccaccatacc atcatcatca agatcatctt ttatcccatt accacaaccc
     4081 gcgacaccat cattatcgca atcatcaaca caatacccat tcccatcaat atcatataat
     4141 accttaacaa ttaacagaag cggattcaaa tccaaccctt tctgagctgt cataatcgat
     4201 acatatcgag atgcatataa tgatgatacc ggattatttt gattgaatac ctttgctcct
     4261 gttattttgc catcaccggg atcataatcg tcatacacaa actgttttaa cgcctcaaca
     4321 gtatcatttc cctgtttata tacaatatca ccatacagat taaatatgaa tttaatgata
     4381 tcacccagcg tccccatttc aagaacatat ttacccagtg gcgaccctct atgaggtgaa
     4441 gaaatgctca tgagcacttt cacaacctga taacccttcc tttgatacaa aacttttgct
     4501 gctttacgaa tatccatgcc accctgagaa tgccctacta aatttacata ttttgcacca
     4561 cttgttgcca tgtagccttc aatctgatca gccaactgag caccacgata ttctgaatta
     4621 tgcagaggct gcacctgtgc tacaaatgat tgctgccctt tgttaatatt gggattacat
     4681 gttacttcaa gaaaggcatc acatggatct cctacaaaca caccaaaatc atcaccccag
     4741 taattgtaac ccagaatagt atcaaaacca cccattccat gagcaaaaat taccggatac
     4801 gttgttttat caactgcagc aaaagaagtc tgaatacaaa cagttaaaaa cataaccacg
     4861 aaaatgtaaa taaacggctt ttttacaaaa tgcatgcgaa cctcctttta aaaaaactac
     4921 tatttaatac cgactaatcg gtattaaata tgataacata tttttgtcaa taaaaatact
     4981 gtactatata ataaaaaata cccttccata agcatgaaag ggtatttttt caaaatcaat
     5041 aatcttcaat caaattaatc gtaatacgct atataatcaa atactgctgc ataaaattcc
     5101 atctcatcaa acatgtcagg accaacacct ataacatcca aatgatcctg attaacgatg
     5161 tcagcagcag aagtcatctg cgcactattg ggatagttta tgctgcttac ataacccaat
     5221 cctgtatcag ttgtaagata atcctgtcct aaaaatactt catttaattt taacctgtag
     5281 cccatctgct gagagttgat gccaactaga ccatcatcat ctaaatcagt acgtgtgcca
     5341 tcacccttgc ctgcagcacc atcattatca cagtcatccg tacagtaccc atcgccgtca
     5401 atgttataaa acccttgccg caataagaat aacgcaggat ttacagccac acccgtctga
     5461 gctgtaataa ttgaaacata tcgtgctgca tacgttgaac tcacaggata agtattgtta
     5521 aataccttca tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgtggatag
     5641 atatcataat aaaatgaact gtaatccttc aagctggtca ttaacactat attcatcaca
     5701 tcagttgtta ctggcttcta ccatattttg aaatccattt ccacattttc tttacgactt
     5761 gttggatttt gccttccacc agttagctcg cgtgtacggt tagcaacata gctcatgagt
     5821 tcagaaattg taataatacc atctttattt ctatcagctt ttttcatggt taaaccttct
     5881 ataacactga acgtgaacac tccatttttc cattgtgccg attcatacgc atattcttcc
     5941 ccacctgcgg acgatatgac tatcgctcca ttatgccttc tcaaatcagc aaataattct
     6001 ttctatggtt atacatatta tttctttaat agaaataaaa tcggatttgt taaaatagct
     6061 tgatttatta tatactgcac gtcattgtga cactagcttg cttatgaagg attgatgtgt
     6121 tatgaaaaat attcttttct attccgaaga tctagcccag gtagagtact cacctacaca
     6181 tccgtttaag ccaatgcgtg caaaacagat gatggattta ttaaaccgat atgggctgct
     6241 tgaagataca caaatactgg aacatccttt ccttgaggaa gaattgttgt acctgtttca
     6301 tacccctgat tacatacagg cacttaaaac ctgtgaacat ggtgaatttg atattaacct
     6361 gttaacctat ggactgggaa ctcatgacaa ccccatcttt aagggagtat acagtttttc
     6421 ccttcaggct tctggcggaa cgtttttagg tgcaaagaaa ttagttgaaa ccgatgcaac
     6481 atttgctttc aatcccattg gtggctttca tcatgccatg cgtgaccatg ctgaagggtt
     6541 ttgttatata aacgatctag ccatagcatt gcattatctt atcaaaaaag gatttaaatg
     6601 tgcctacata gatattgatg tacaccatgg tgatggtgta cagagtgctt tttacgatga
     6661 caacagagta ttaaccatat ctatccatga gagtgggcag tttttatttc ctggtaccgg
     6721 ttttgaaaac gaaattggtg aggggccagg atacgggtat aatgttaaca tccctctttt
     6781 aaaagatacc gacgacgaaa tttatcttta tgcctttgaa gaaattgtac caccactcct
     6841 tcaaagtttt aaacctgata ttgtatgcat tcaggtgggg ggcgatagtc ataaggatga
     6901 tcctctggcg caccttaatc ttaccagtaa cgggtacgaa aaagtattaa cactaataaa
     6961 gcaatactcc ggcaaagtac tgggaactgg aggtggtgga tataatctgt ataaaacatc
     7021 ggcactgtgg acactggcat ggtccacatt tgcaggcatt aaacctgtgg ataatttcat
     7081 gggtgttgta ggcggcatga tgtttggacc agaaatggac gctggctcac tgtacgatac
     7141 cccttatcat attggcggat tgacaaaaga acaatgtttt tcatacgcac gcgatgtggt
     7201 ggattatata aaacagacag tattccccat tcataaaata taattcaagt gtaagccagc
     7261 tacttgcagc atatgggtaa ccgtattctg actatcttta tttttacttt ttctatagac
     7321 gcttgatggc agcctcaact ccattcaatg ccaccagaat agaagcataa tatccgcttt
     7381 taaatgtccc tgcattaacc acggtggtat tgcctaactg agctacacca tgcgcttcat
     7441 gtatgtgccc gcaaatacat aggtctatgt tataatccag acaaaactgc gcaatcgctg
     7501 tacttccagc attggttttg aagatagttc tgtcacaaac attttttggt ggattatgcg
     7561 atataagcac cttaattggt tgatttttta tatgttcaaa acctctgcac aaaaagttat
     7621 atatttcatc ctcagtgtat tcaacaggag tgtgcagcgg cgttttaata gagccaccta
     7681 caccaaaaaa accaatatca tcaactatcg caaaacctgc atgaatggaa tacggtgcaa
     7741 ggaactcttg gattctactc ccatcccagt tgccatgaac tgccaacact ggtttatgta
     7801 tctgggcgat agctccttta taagcctccc aacgcgtata ccacttcagt ggaccaatgt
     7861 cccctgcaat gacaacaata tcactggatt caatctcagg ctgcaagcga tagatattgt
     7921 tatatccgcg gtgtatatca ctgatagcgc agatttttag cattttcatt catcacaata
     7981 tatatttttt tacaggcaaa cttaatgttg gaatcctggc gggatatgga taaatgtcaa
     8041 gaactataca ccataatgtg gaatttatag tgtactatat atataattat atgcctctgt
     8101 cctcgtcaaa tatttcggca ggtattaata cgccaaaata tttaattgct gctacaagcc
     8161 tgtcgcattc gtcaacctgt ggcagcgttg cacattgaaa aggttcttca tattccctgt
     8221 agatgtataa ttgtgcattt ttcatggtaa taaccctgta ttaatcatcg taaactaata
     8281 tagcataaat tacattaaaa tcaagctgaa ctttacaaaa attatttaaa atatatttaa
     8341 atttgtattt acacgcaagc gggtaaaata tatagtatta ttacattagt ataatatatg
     8401 ttataatcaa ataacgcttg ccttacaggt agtgtttttc atatcatacc tgcattatgg
     8461 taacgcaact aacattattg gaattttcta tcattactct tactgtatct ctttccgggg
     8521 ttatggctcc tggccccatg agtgcagtaa ccatcaatca cggtacacgc tccaggctgg
     8581 cagggttaat ggttgcaact ggacatgcaa ttgttgagtt gccggtaatc cttgcattat
     8641 tttttggcat caagctcata acacagttta ccaacatcac aacatatatt accttagcag
     8701 gaagcattgt gttattcata ctagccacgc ttacactaaa aagcttacgg aataacaaca
     8761 cctcagtaca atcatataca cccatggtcg atggaattgt cctttcagca tttaaccctt
     8821 atttttacgt gtggtggctc actattggac tatcgcttat acagaaagct tttgtatttg
     8881 gcacagttgg attaatcatt ttttcggttc tccatcttgc ctgtgattac atctggcttt
     8941 ttgtattatc aataatttct tacgcaggtg cttcattcat aggaaaaaaa tttaaaatag
     9001 cagtaagtat tattacagtg ataatgctgt atgcatttgg catattcttt atgtacgatg
     9061 cactacacgc tattgtctct caataattat aattatacta ctacaaaggc aatctttata
     9121 aatttctatt gacacctcat aattatagta gtacattaat cttgttttat agtatcatat
     9181 caaaatatat actaactatt ttgaggtcat acatgaataa caatactcaa aatactattg
     9241 cccagctcaa agaggagttg attcctctta ttaaaaccgt tgtgcaggat gaagttaagt
     9301 ccaatattga agattttatg cgtcaaagcg aattgcgagc aaaagagcta agccttattg
     9361 aaagaattat cagggttgag gaagaaatca aagcattaaa agaaattgaa aaagcccgtt
     9421 ttgaagcaat ggaaacacgg ttcgaagcaa tggaaactcg ttttgaagct attgaaaagc
     9481 gtttttcatc gcttcaatgg tttatgggta ttggctttac tattataagc attctgattg
     9541 ctatatttgg tcttctcaaa tgagatatcc tcacaccatt accgtgggat tattctttta
     9601 acagaccgtg tgctatcaag cgtcagatcc acggtaaatg gacgattaat cttttgctgt
     9661 ttgtcatttg tcaaaatttt aacgatgggc cgcagatcac ccgttgagtg cagctcttca
     9721 acctgggcaa cttcaccagt agtaagcata acaatagtac caactggtag cagtggttct
     9781 ttttgcaaaa gcggcgtcat gtctcgtaca aaccgtgaaa ccagaaccgg attaaacttt
     9841 acccttgact ctttcatgat agaaagtatt gcctcacgtg atgaatacgc tctcttgtaa
     9901 ggacgaggtg tgataagcgc atcatacaca tcacaaattg atgttatcct tgccccaatt
     9961 ggtatttctt catcctgaaa cccttttgga tatcctttgc cattataatt ttcatgatga
    10021 tgcagtatta catcaagtgc atttggcggg atatgatcat gttttgccac atattcatat
    10081 ccaaattcag ggtgcttttt aataatctcg aactcatcac tggttaactt ttctttttta
    10141 ttaagaatct ctttaggaat cattagtttg ccaacatcat gtaaaaacgc accaagccct
    10201 atttcacgta ccagtttttc ttcaagccca tgcttggaag ccatggtcat tgctaaaata
    10261 cccacattga cagagtgggt aaatgtataa tcatcataat ccttaatttt cattaaataa
    10321 agaaaacggt ctttgttttg ctttacatat tggtgcagtg ataacacaaa atcttttgtt
    10381 tgctccagat caggccgtgc atttgtcctg acagcttcaa acaatgattc ccagatatcc
    10441 tgcgcctgtt cagtaaatgc cgttgcatca ttattttccg ctggcgtata atatatacgt
    10501 tttattcctt tcgctttcag cgtatcaata atttcactgg tatacacagt ataggcagga
    10561 tataaaaaat tattttcaga atcaaaagta tctccggaaa gcgttgcacc cggaaccata
    10621 aattcaacaa taacctctaa acgtttagcg ctatcttcca tactagcaac accttattta
    10681 tggtaagcca cagcatctcc aatagctata atggcatcat tgttcccacg cacctcaagt
    10741 cgccaggaac ttattttcct gccagcatga accagcatgc attcggcaaa tatccgctca
    10801 ccatccaggg ctgcctgcaa atattgcagt gtcatggtaa gagccaccgc catagttccc
    10861 atggaattgc ttgccactgc aaatgcctga tctgccacag catgaataat accgccatgt
    10921 gcacgctcta ccgcattgag gtattccggc tttacggtta aaccacagcg cgcataccca
    10981 tgctttacat cctccacttc aatccctaaa aatgttgcca gaggatcttt ccccacaaca
    11041 tccctggcat agccaatggg atcctgaacc atcttttcca ttattcacct caaaattttt
    11101 cattaatttt atcacacacc atattaccaa tcaacttttg tgataactcc aaactcctct
    11161 tttgggggat tataataccc taatgtaagc ctgggatatt ttgtttgcaa aagttttagt
    11221 aactgtcgca cacctattgc ttcttcatct tcaaaaccaa tactgtccag ataataatct
    11281 ggatcaatat tcagattacg ggtttgtatc atgttcaccg ggaagttgtc aagaaaagta
    11341 cataaacgct caacctctga ctgtgcgtca gtaaaaccgg gtaaaaagaa aagatttatt
    11401 gatataaaaa taccagcttt taaagccacc tcaatactgc gcaacatatc cttaaagcta
    11461 taattttttg ggcgatagta cctggtataa tactcttctg ttggcgaatt catgctaatg
    11521 cgcacagaat caagccctgc atcaatacac tgttgcaagc cctgaggcaa tgatccattg
    11581 gtgttcaggt gaatggtacc ttttgaagtt ttgcttcgca cgattgatat ggccgctgct
    11641 aaatcctttg cgcgtaacag cggctcacct tcgcaaccct gaccaaagct aaccactgct
    11701 ctgtcaaccc tctcaatgtg atgtagcatc acttcagcta tctcctgtgg tgagggctga
    11761 cgggtaagcc tgtactgtga tacatgaaca ccagtatctt tttgaaacga aaagcagccc
    11821 aaacacaatg cgttgcatga ctttgttgtt ggcagcggtg cttcatgccg gcttaaaaag
    11881 aaattgcgcg cacacaggca cccatactga gtggagcatt ttgcaagctg gcgcaccagg
    11941 ctgtttgagg ggtactgttc tttaaccact tttatttgct taaacagttc aatatcattg
    12001 cggtgtaatg cggggtcaga gcgtggatca ggatcaatgc gaagtgccgg cacataaaaa
    12061 tcatcaccca caaacacaag gccacagtac gcccataaag aaagaacggg tgcatcctta
    12121 gttttaacat atgcaggcag gtatgttcgt aaataccctg aacttaaaaa cgatgaaact
    12181 gcccagattg tattgccgtt atagtgcgtt attgttttga aattctttcc ttcaacagga
    12241 tagcgttgtg gtaaaaggta taatgtactg ccatcaggga taggtataag ctctttttca
    12301 tccaccttca caaaccggcg gcctgacctg aaagctgcca aggctcccgg atgtttgtac
    12361 acattaccct caatatcgca aaatgctaaa tatgatttct tcataccttt gcgttttaca
    12421 aatcaagtat accaatccgc tttatacatg cagataattt tttattatat tctttttctg
    12481 catggcgcat gcgtgtgcgc agtgtattct gctgcctttg tagcttttta tattgtacat
    12541 ctttttttat tgcagcttta tcctttccct gcttcatcag ggtttcttta tagcgcaacc
    12601 gtttgtcacg ggtactgcgc cactcgtgtt ttaataactg catctttttt ttaatgggca
    12661 atagctcatt atcaaaatat tgcctgaaaa cttcatcact tgcagcatga aactttgcca
    12721 gcttctgtaa caggctatca cggtatgctc tacctttatc ggttagcgtg cagcaattgt
    12781 cgtttatgga tatatatcca gctttgatta aatcttcagg cacttgccgg ttactatcgc
    12841 cgcaacaaaa aagcaggaac ataatgtgtt gcaactcctc atgtgaaaga gaggattcca
    12901 ttaaagcagt acgtaatttt tctatcatcc cccaccaaca aaccgtgcta tttcaaggac
    12961 atcgccatcc tgtaatgttt cggtggcata gttttccttt tccacaatac ggccattctt
    13021 ctccaccatg atttttgagg gattaaatcg gtacaaatca agaagctctt ttaatgtggt
    13081 tttttcaaat tcaatatcta tgccgtttac cctgatcctc atactgtttc cttttttacg
    13141 tatagcccca ctcatatata ataatggcag gtaatactat agctgctgtt tcagctctaa
    13201 gctgcgttgc tccattatat aatacaatcc aaccaaactg tcgtgcaatt tcaacctctt
    13261 tttttgaaaa gccaccctca gggcctgtta acagcataac atcgttatgc tgcggattat
    13321 ctttcaaata ctgccgtaaa ttctgtgctt caggatcagc atgtgccagt ataccaacgc
    13381 ccttagataa ttgtagcaca tcagtatatt ccatcacgga actgacccgg ggaacatctt
    13441 ttcttaaaca ctgctttgca gcttcagtgg caattttttg ccagcgttct aacttttttt
    13501 cttctttttc agaaatatcc ggtatggtac gttcagtcac aacaggaatt atgctacgta
    13561 cccctatctc cactgctttt tgaatgacaa gatcaaattt tttgcctttt aatagcgcta
    13621 cacacaatgt caggtttaat gaaatttctg gatatgtaat ggtctgcatt atagtagcaa
    13681 taattttttg tgcttctatg tgtgcgatag ttgctctgta gcgtacgcct tctgtatcaa
    13741 tgacatcaat actatcacca acacgtacac gacgtacccg cactaaatgg tagtaatcct
    13801 taccttcaat aaccactgta ttatcatgac ccggcgcact gacaaaaaat tgtgccacgc
    13861 taatccccta atttttcagg cctgaatatt tctgtattaa tggttgcccc atcttctata
    13921 gaagtaaatt caactacagt cattgtgcct ttcttaatgt tcaacatatc atatcgtatg
    13981 gggaatgatt taccgtcttt ttgcccaatc tttgcaattg aaagggtctt aaatattact
    14041 ttatcctgat cgtgataatc gattcgtaat ggaatataat tttgtttgat aacatataat
    14101 gtaagtttac catacgcacc tgttttatca ataggatcca gtgtcagttt ataacagtcc
    14161 tgatttttca aaataaccga accatcaatg cgtgcaacat aattactctg taaatctgca
    14221 ttggataaat caacataaaa aaagttggtt gccagtatat catcatattt atcaataccc
    14281 atcttatgaa aaagtgcccg tgcagccatg tcatataccc acaggtcttc accgcctata
    14341 cggtacagta tcttttgcat acttccccgt gctggtttta taatatcaaa taaaaactga
    14401 ttcttattga tataccctgt tatctgtaat ttttgggatg acccatcagg tgttatatgc
    14461 atcatatacc ctttgataat cccatctgga tactccataa tgcgatcaac tcgggctaat
    14521 atttcctgtg cggtcagttc tatctctcta tcagcctggg caacactatg atcatgtgaa
    14581 aaaaagatga tactgacacc aagaaggatt actaacatta tactgtggat attcttcatt
    14641 gcattttccc ggcacacatt attacggata tcacttttta tactttcgca gttttaaaat
    14701 aagtaaactt ttttcatgcc gcatatggaa agtagccaaa acaatagaat aaggatacat
    14761 catacaacat tatatttgca aattattttt tatgtctggc tatctgatac ataatattta
    14821 tcctgttctg gtgttcctat atcaggcctc aatttccttg catcccgcag atacacatga
    14881 actatctctt caggattttt atcagtattg atatgcagca aaaccctcac gcacatacca
    14941 agtgatcccg gaaccggaat ctcacgggaa cacaacagtg gtatatgaat ccatcctaac
    15001 ttacgcgctg ctaccgctgg aaattctgca ttcagatcat cggtcacagt aaaaatggct
    15061 gaggcaatat cattggtctg aattttattt acagtaacta tcgcccgtaa aagttcttca
    15121 gttctatgaa ctatctcctc ttttgaattt tcgtcaacgg taattgcacc gcgcactccc
    15181 cgtacaacca tcattcctcc ttgcgtatgc tggcacctaa cgcctgcata agctctacaa
    15241 aattgggaaa tgtaaccgat acggattctg cagtatctat ggttgtgcta ccctctgcag
    15301 caagccctgc aacagccagt gacatcacta ccctgtgatc gtgatggccg ttaacatgtg
    15361 caccttccaa tgtgcttttt cgtatgataa gtccatcagg caattcctca atatctgcac
    15421 ccatcttttt taattcggca cacatcactg caatcctgtc agtttccttt acccgtgcct
    15481 gtggcacatt gacaaggcgc gtttcaccat ctgcaaaaca cgcacacact gaaagtgcag
    15541 gcagcgcatc aggcattgca ttgaggtcaa acacacctcc tttcaacggt tttccactaa
    15601 tggcgatagc atccggcata atatcaacgt cagcacccat tgccttcaaa atattcacta
    15661 cttccttgtc accctgtgtg tcattaaaat caagcccctt taataccacg ctgctttgcg
    15721 ttatggctgc tgccaccatg aaaaatgttg ccgaagaaaa atcagccgga atgtatttgg
    15781 tgaagggttt atagctttgc ccgcctttca caataaattg ttcataacct gtatggtgat
    15841 actctatgcc aagttcttta agccaccaca acgtcattcc cacataagga gcttcattga
    15901 ggagaggtac atctataatg gtattgtgct ttgcctttgg tgttgccacc agcagcgatg
    15961 aaaggtattg agaggtaata gcgcgtattg aaacatgtcc gccaatgatt ggtccctgta
    16021 ccacaactgg cggtttgcca tttgaacggg tggaaacagc gtacccacct aattcagtaa
    16081 ttgcattgag taaatcttca gcagggcggt tacgtatctg atgatcccct gtaaatactg
    16141 tcattccagg tataagtgaa gccaccccta taccaaaata cagtgatgta ccggaattgc
    16201 caacatcaat aacgtcatca ggtaattgag gtgcaccatt gctttttacc acccattcat
    16261 gctcatgttc ttcgacggtt gctccaaact ggcgtatcat gctaaggcat gaacgcgtat
    16321 cacttgaatc cagtggcatc tggatggtag atgttccatc agcaagcagc gcacatacca
    16381 gtgcgcgtat ggtatgtgat tttgaacc
//
LOCUS       DAORYL010000069        16351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_2895_length_16351_cov_12.549828, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000069 DAORYL010000000
VERSION     DAORYL010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16351
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2895_length_16351_cov_12.549828"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1559)
                     /locus_tag="PLV81_11615"
     CDS             complement(<1..1559)
                     /locus_tag="PLV81_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10787.1"
                     /translation="MNFKILFVAIVTVIAFTSCGALQEDTAVIAGSVMVDEGISRASS
                     APVFVAIARNGDMESIQNDPANTIITVIQPDDSGDFSIECKDYGLKSGDEIFLFAFID
                     NDYAGGIPYPTPGDAVGFYNNGFKLSYTIGVDGQATILINRQQYDFDANIIGILDGTE
                     SGNVILIAYAGDFNSSNFSDIDIDAVIGYKKLTKPAYPVSFTLPVMPYGYNVPIGGVY
                     IIALLDANANGIPDEGDTIGFAVEPGSNTPVAVTVTNGVVSASTIKFVMPIYGEPATN
                     GPPLTITGQFDAPEGYSSDPTTKPIFVVVARGSDPNEVLTNIKNLNTQTFDFTRVTQG
                     ENTFALTLSRSKFNPGDQVFIFALWDKDYESGLPSPTQGDKLGFVMNKSNFAYTVTLQ
                     EGDNQLMKSGTQYLFNGNGGYSFSLNRTIYNHNAAIRFQLEKGSLNDTQFANGTKVIV
                     VAVYETNDIAQQYLQYGTYNLDMDKIIATANVRINKESGSGTTVYYTMPVMPALHESI
                     PATQDEQLFID"
     gene            complement(1549..2163)
                     /locus_tag="PLV81_11620"
     CDS             complement(1549..2163)
                     /locus_tag="PLV81_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10788.1"
                     /translation="MPVKTILQPNEPVSILVEPRYAIVNMAGTALSKAYKDFVFQVEA
                     VYSPDKTGVVDVNLQNLSAAQLQTLLPYKTRKSHFVTYSAGFNYFIPIRDYFKEHEGQ
                     CVFTFEWSQSHFFDNKLMKPMLSDIVVLRLQDSFFNDKLDLKISSFVEKQTSSYMIMP
                     RVSYRFDSGLTFDVTYVYLHMTADSYFAGYNNNDCVTGRVSYEF"
     gene            complement(2191..2976)
                     /locus_tag="PLV81_11625"
     CDS             complement(2191..2976)
                     /locus_tag="PLV81_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10789.1"
                     /translation="MNYWTHIFFWAIVTILASGLAFAQEPFSLLSVEDKPTLSIKGYV
                     EIDTTTSTYPHQETADIFKKDELKANTTIKYGKESFYVYTDINAYLLPNLINENINPT
                     YRYSEETDILRNGTITSSNAEINFRECYINYEIGSVRLRAGNQIFAWGTADAFNPTSY
                     FNTYDLREFLFKDEDTELKMGVPAVSMLMTLGNDTLELVITPLHIPLRMPQAGSFWQL
                     TVKEGPFPVTIAQPEALPFEIANAGVGARYYHNIAGFDVHLSA"
     gene            complement(2973..3566)
                     /locus_tag="PLV81_11630"
     CDS             complement(2973..3566)
                     /locus_tag="PLV81_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOM10790.1"
                     /translation="MSHTIIDKRQQILDVAKELFATKGYSATGIREIAEKAGLSLGNF
                     YNYFKNKEDLFKSLLDPQNILEHLTEIKLMLNDGFPFNFKDIIKSIKTVVDSDFHLYR
                     LIFIDLTEFNGMHTDKILDRIITEATATFNANVKNSIVGTVIKDEDYQFYIKAFIIST
                     LSLLVIGNILPSANLNDKSNDYIAEKLSNVILHGILL"
     gene            complement(3766..5553)
                     /locus_tag="PLV81_11635"
     CDS             complement(3766..5553)
                     /locus_tag="PLV81_11635"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOM10791.1"
                     /translation="MFWTFFKDGVKKYGNKVLLNHHNGTQWESFTWNQVDTMVKALAV
                     YFIDLGLKPGDVVSIYSENRPEWVIADLATLSIGGIDAAIYPTNSAPEAAYILKDSSS
                     VVCVCSSKFQADNVLSKKSELPNLKKIIVCDDINYNDDMVITFKDAVNAGNKNLHIEE
                     IEKRTKNVKPDDVMTLIYTSGTTGDPKGVMLTHKNIMFICLTFNKVEDLPEGFIHLSL
                     LPLSHSVERTMDYYSVLERGAVIYYSRGTEYFAEELKEIRPQCDILVPRVFEKIYNGV
                     MAKVKEMPEKKQKIFNWALSVGLQAAPYFMAAKRKPPVLAVKYAIANKLIFSKLKNAL
                     GLDRVICWGAAGAPLAKEIHDFFWAMNVQVRKGYGLTETSPVLAVDGSPKIKPIKSDG
                     WICPFPETQIKIADDGEILAKGPQLMKGYLNKPEATKEMFTDDGWIKTGDIGVLDSEG
                     YLKITDRKKDIIVTAGGKNIAPQVIEQRLLIHPFIEQVAIVGDARKYIVALIVPNFET
                     LTQWAATQGISAPSNDELVKHPDVRKKYEAIVNEVNQEFGKVEQIKKFTLLPQQFTQE
                     TGELTPTLKIKRKVIQKKYADIIEEMYKE"
     gene            6082..7647
                     /locus_tag="PLV81_11640"
     CDS             6082..7647
                     /locus_tag="PLV81_11640"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOM10792.1"
                     /translation="MKVLLINPPYPFEESPSPPFGLMALAAYLEEKNIEVKIEDYIVN
                     RFSENRVQDVMSSFTPDVVGSTGVTMNIKKALAILKVYKQINPDIITVLGGPHATFDA
                     HNMLLHHPYVDVIVRGEGELTFEQLLSHLNNKNAYAEIEGISYRDNDNTIVHNPNRDF
                     IPDINILPYPARHLVELSKYRALGFPINMMTSRGCPFNCIFCVGSKMVGRKVRYFETN
                     RVVDEFAMLATMKFKQINIVDDLFTSHKSRCIEVCKEIKRRGINHPWTAFARVDTVNI
                     ELLEHMKDAGCTMLCFGIESGNQEILDKVKKKITLEKCKQAVAMCRQVGISPMTSYIL
                     GLPGETEETIKKTLEFAKELSDNYGFHVLAPFPGTEVREMKDYYGIEILTNDWDLYDA
                     NRAVSVTPWITADRINDIVDRFNGSIKTYILSLGEKQNHGQKLSHNEQELISNIRSFE
                     IVRDMILDRLIEEFPGIENGVPVDVKKEFVEYLSQKTKFSKEELHNQVERLVELQCID
                     FKYRNSTIYPVWT"
     gene            7780..8259
                     /locus_tag="PLV81_11645"
     CDS             7780..8259
                     /locus_tag="PLV81_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10793.1"
                     /translation="MNFETTTCISKEHYTLLCYYSEKYNVKLSKLITALLRFVGEHQK
                     VYFTTNRRIKYRERQGCNWKRIHIMLTPYDYEIILDLKKYGKMSLAKMIEFSIENYFE
                     RFVDRVLKKQSTDNYLYANYHFEVTTENNIFSYSVYWGLPPKILQKLTKTNHLFFKT"
     gene            complement(8273..9109)
                     /locus_tag="PLV81_11650"
     CDS             complement(8273..9109)
                     /locus_tag="PLV81_11650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOM10794.1"
                     /translation="MLKNKGEKQVIVRDKNFLQDYIKVWDDGAIKDAPFITLGLEAVL
                     TDYYGNLLDAPPPFEVKPDETILDLGCGWGRVLKPVRAITDNAIGLDISHQMLMAAQK
                     HLHHHNLPTPLVRGDGTLLPFKDCSFDTVYSLLVLQHLSKENGKKVIKEIHRILKPGG
                     TAYIRVPSRFSPENLLMAFLQLVSIHIFRYKDPIRMRFYRIGEIHKICKPLFNTVEIT
                     AHEFRPPWNFHTPWTWHYIIIPKRFHKRLRVLSDKLEKRANSKSPWFKHFGVTLMIKV
                     VK"
     gene            complement(9066..10550)
                     /locus_tag="PLV81_11655"
     CDS             complement(9066..10550)
                     /locus_tag="PLV81_11655"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HOM10795.1"
                     /translation="MTMAYKGKIVKLVIVLSILLVIIFQWHRLIYIANYIKGYYITIK
                     DSLFAPDTVSIIYPTRFFNYKRPYHHAPHELLHCGNMLLYIAPKEERVSDIAQRLIGY
                     TYYYRASHLHNAIVTLNALTSKTVGAGSVLVIPHPLPFITIDLSKKSMTQIPYVKGVY
                     YSGNSLASSKMMRQLSELKQAGINAVVFDVKDVCGIVNYKSHVPIVVELNTHEKRPVD
                     DVVTLIRNFKELDLYVIARIAVFRDHLLATKNPDMAIKSKRTGGIWNQGSKELWVDPT
                     KQTVWDYNIALACEIATLGADEVQFDYIRFPTAGDLSDAQYAYDFGKKTKVETITEFL
                     AKAKNELHKLNTAVSIDIFGVAAWQKEEDVSKLGQKIELLARSCDVISPMLYPSHFND
                     NFDGYANPGDEPYYFIATGNKKLQAIAPKLLIRPWLQAFKWRVSNYNEDYILAQVNAC
                     INTNTYGYLFWNASNDYTVVYKAFMKKNNGNAKKQRGKAGDSQR"
     gene            10641..11438
                     /locus_tag="PLV81_11660"
     CDS             10641..11438
                     /locus_tag="PLV81_11660"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOM10796.1"
                     /translation="MVQIIKKNIFIIVQKVDGIIKGIADFNAYMKAIVRSFRSLRYLK
                     IRSLYTIAVNQTRFTGIDALPLVSWIALLLGATVIIQATTNFPKYGIESFIGNLLVII
                     IARELGPLVTALIVVARSGSAIAAEIATQTQNREILSLELMGIDTRMYIILPRIVASF
                     AAIFTLIIIFDVIAFFGGYLIALTSVYIPMGTFTQTLLDAFTFNDLLVTITKSIIYGV
                     LIPLICCYYGLKPKSSFEIPIFVSKAVIRTLLIIFIINAVISVLFYF"
     gene            11441..12208
                     /locus_tag="PLV81_11665"
     CDS             11441..12208
                     /locus_tag="PLV81_11665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOM10797.1"
                     /translation="MECEEIKQLIEVKNVTIEGGKQNLRNVSFNVCLGEDVVFFGAED
                     SGIDYICPLLAHALEDYSGEVLYKEQHIKHMDFVEVHNFRKIFGYVQRGYALISNMTV
                     YENIALPLRYHTDLSEKEIHELVDSLIVYLHLSHCRDLRPIMLTQSEMLRTAFARAIA
                     LDPDMLLIEHPLEGQCLWNAQIFLQALQNRAFAPNKTVIVVTYQPLVYIDIATRFIML
                     HEGSIVFDGTKEEFQTFQNEYVKQFVDVLPYGPMTLR"
     gene            12220..12903
                     /locus_tag="PLV81_11670"
     CDS             12220..12903
                     /locus_tag="PLV81_11670"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOM10798.1"
                     /translation="MKLEKNEIRVAIFILLPLVILLVFVVLKLGYSFATSTIDVYMKV
                     DNISSIKNGTQVKIKGYTVGRVVDIIPVYKPTLHFLALLRIKKEIDMYEDCSAVIMNQ
                     NIIGDTVIEIRNPVKKEALLRPGAVIEGVEYVSLETIVQDVHALLATLTNTVAVIQDI
                     SLESKGNIRNLVANLSKSVETISSVLQDSQKDILAIMASFRETAKTMNEISIELKKHP
                     VKFLFKGEK"
     gene            12907..13779
                     /locus_tag="PLV81_11675"
     CDS             12907..13779
                     /locus_tag="PLV81_11675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOM10799.1"
                     /translation="MPFFSVVIPTFNRAHLVSRAVESVLAQKFTDYECIVVDDGSTDD
                     TQRILSPYKKHIQYMYTRHGGVSKARNYGILQAQGEWIAFLDSDDMWLPKKLAIQYEY
                     IQTHPDVYIHQTDELWIRNGRRVNPMKKHQKKEGYIFEDCLYLCLISPSAVVVHRSVF
                     ERVGMFDELMPACEDYDLWLRVAWQYYIGFIPQRLIVKYGGHSDQLSSALWGMDRFRV
                     YALCKLIASYGLLLPEAYFNKAVEVALQKCSVLYQGAKKRSNVALIQKVERVVQWLTV
                     DRKSTSVYPDLLEE"
     gene            complement(13727..14797)
                     /locus_tag="PLV81_11680"
     CDS             complement(13727..14797)
                     /locus_tag="PLV81_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10800.1"
                     /translation="MRYSIIGLLVGMLLGFSPVFMTHTGFMLTPLWYGSLQKIQAIEA
                     APGNDEKMSFAVTTDSGYYHIGASGLVHKFITAGDAFVKPTSDGKFYITYKKVGNEIA
                     LYNASNEFFWKLPSTEYPYISPDGSIIILLTADGSSARIADFNGNVYPHDELNGQFTT
                     TIAFSQKGSFAGIGFIDGRYYICNNTGQIIYKGTSPSSTIVKGIAIDDTGKYALVHCG
                     NSSKDYVVGINITRQKSFAYELSAPHYVKTSMAINTYGKALFLDFEKLLEIDIKGKDV
                     TPYDIIKKRYGQSQVSGNDDIFMISYPADAGGSVWYVWQWGKGIVYKREFYEEPFLHH
                     ILSHNIILLRGQDTLMCFSYQQ"
     gene            complement(14807..15781)
                     /locus_tag="PLV81_11685"
     CDS             complement(14807..15781)
                     /locus_tag="PLV81_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOM10801.1"
                     /translation="MPKKVKQEKLTKSNIMSAPESQAKKLKLQKQNKVTKNNNEAIKA
                     NFDDFTDSSISWYLEQINKIPLLTREEEDALARRARAGDQKAKEELIKANLRFVVSIA
                     KKYQTSGISLLDLINEGNMGLIKAAERFDPDRGYHFISYAVWWIRQAILLAISQKTSL
                     IRLPLNRTADMQKIDRVHKSLESKLGREPSPAEIAEELDMDNDEINHLRNITQDFVSL
                     DATIGNSDDNTVLSLVEDLKVESPDKKVFDESLKEALNEVLDTLTPSEREILKLRYGL
                     DGNEPMSLQQIGERFKLSKERIRQIEKKAIRKLRLPSRSQKLKSFLTD"
BASE COUNT         4836 a         3033 c         3240 g         5242 t
ORIGIN      
        1 ttatcaatga aaagctgttc atcttgcgtt gccggtattg attcatgtaa tgcgggcatt
       61 accggcatag tgtaatatac cgtggtgccg gagccggatt ccttattaat acgtacattt
      121 gccgttgcaa taatcttatc catatcaagg ttatatgtgc catattgtaa gtattgttgc
      181 gctatatcat ttgtttcata gacagcaaca actatgacct ttgtgccgtt agcaaattgt
      241 gtatcattaa gacttccttt ctcaagctga aaacgtatag cagcattgtg attataaatc
      301 gttcggttta aagaaaacga ataccctcca ttgccattaa ataaatattg tgttccgctt
      361 ttcattaatt gattatctcc ttcctgcaat gttacagtgt aggcaaaatt acttttattc
      421 attacaaatc ccaatttatc accctgtgta ggggaaggca gaccgctctc ataatcctta
      481 tcccacagag caaagatgaa cacctgatca ccagggttaa acttactgcg tgagagggtc
      541 agagcaaaag tgttttcccc ttgtgtaacg cgggtgaagt caaatgtctg ggtgttcagg
      601 ttcttgatat tggtaagtac ttcgttgggg tcagagcctc tggccaccac tacaaatatt
      661 ggctttgtgg tggggtcaga gctgtatcct tctggagcat caaattgtcc tgtgatggtt
      721 aatgggggac cgttagtagc aggttcaccg tatatgggca taacaaactt aatggtagaa
      781 gcagaaacca caccattggt aacggttaca gctaccggtg tattgctgcc aggttcaaca
      841 gcaaagccaa tagtgtcacc ttcgtcagga atgccgttag cgttggcatc aagtaatgct
      901 atgatataga caccaccaat tgggacgttg tatccgtatg gcataacagg aagggtaaag
      961 cttactggat aagcaggctt tgtaagcttt ttgtacccaa taacagcgtc aatgtcaata
     1021 tcagaaaaat tgcttgaatt gaaatcccct gcgtaagcaa taagaatgac attacctgat
     1081 tctgtgccat caagtatacc gatgatgttt gcatcaaaat catactgctg acggttaatc
     1141 agaatggttg cctgtccgtc aacgccaatg gtgtatgaaa gcttgaagcc gttattgtaa
     1201 aagccaacag catcgcctgg tgtggggtag ggtataccgc ctgcataatc attatcaata
     1261 aatgcaaaca ggaatatttc gtcgccgctt tttaaaccat agtctttgca ctcaatggaa
     1321 aagtctcctg aatcatcagg ttgtataaca gtaataatgg tgttggctgg atcgttttgt
     1381 atggattcca tatcaccatt tctggcaatg gctacaaaaa caggagcgct tgaagcacgt
     1441 gaaatccctt catccaccat gacagagcca gcaatgactg cagtatcctc ctgcagtgct
     1501 ccgcaggaag taaaggctat aacagtaact atcgccacaa agagtatttt aaaattcata
     1561 gctaaccctc cctgttacgc aatcattgtt attgtaccct gcaaaatacg aatcagctgt
     1621 catgtgcaga tatacgtacg ttacatcaaa ggtaagacca ctgtcaaagc ggtagctgac
     1681 gcggggcatt atcatgtaac tactcgtttg cttttcaaca aatgagctta tttttagatc
     1741 aagcttatca ttaaaaaagc tgtcctgtaa tctcagcaca acaatatccg aaagcattgg
     1801 cttcatgagc ttgttatcaa aaaagtgcga ctggctccac tcaaaggtaa acacgcattg
     1861 tccttcgtgc tctttaaaat aatcacggat gggtataaaa taattgaatc ctgctgaata
     1921 tgttacaaaa tggcttttgc gcgttttgta gggaagaagc gtttgtagct gtgcagcgct
     1981 caagttttgc aaattaacat ccacaacgcc agttttatcg ggcgaataca ccgcctcaac
     2041 ctgaaataca aaatctttat atgcctttga tagagcagtg cctgccatat tcacaatagc
     2101 gtagcgtggc tctaccagaa ttgaaacagg ttcatttggc tgcagtatgg tttttacggg
     2161 caaaagatag ggtatagtat cggggccgtg ctatgcactc agatgaacat caaaaccagc
     2221 aatattgtga tagtagcgtg caccaacgcc agcatttgca atttcaaaag gcagagcttc
     2281 aggttgtgcg atagttactg ggaatgggcc ttctttcact gtaagttgcc agaatgaacc
     2341 tgcttgtggc atgcgcagtg gaatatgcag cggtgtgatg acaagctcaa gtgtatcgtt
     2401 gccgagggtc ataagcattg acaccgctgg aacgcccatc ttgagttcag tgtcctcatc
     2461 cttgaacaga aattcacgaa ggtcgtatgt attaaagtac gatgttgggt taaatgcgtc
     2521 ggcagtgccc catgcaaata tttgattgcc tgctcgcaaa cgtacagagc ctatctcata
     2581 gttgatatag cattcccgga aattaatttc tgcattgctg gacgttatgg tgccatttcg
     2641 caggatgtcg gtttcttcag aatatcgata tgtaggattt atgttttcgt ttatcaggtt
     2701 tggcaacaga tacgcattta tatcagtata aacatagaat gactctttgc catatttgat
     2761 ggtggtgttt gctttcagtt catctttttt aaagatatca gctgtttcct gatgggggta
     2821 ggtcgatgtg gtggtgtcaa tttcaacata tcccttgata ctcagggttg gtttgtcttc
     2881 tacggataaa aggctaaagg gttcctgtgc aaaggcaagc ccacttgcta aaatagtaac
     2941 tatcgcccaa aaaaatatgt gtgtccaata gttcatagca aaatcccatg caatatgaca
     3001 ttggaaagct tttctgcaat atagtcatta gatttgtcgt ttaaatttgc tgatggcaaa
     3061 atatttccta ttaccagaag tgacaatgtt gaaatgataa atgccttaat gtaaaactga
     3121 taatcctcat cttttatgac ggtgccaaca attgagtttt taacgttggc gttaaatgtt
     3181 gcagttgctt cggtgattat tctatcaagt attttatcag tatgcattcc attaaactcg
     3241 gtcaggtcaa tgaatataag cctgtacagg tgaaagtcgc tatcaactac tgtttttatg
     3301 gattttatta tatctttgaa attaaaggga aagccatcat tcaacatcag cttgatttca
     3361 gttaaatgct caaggatatt ttgcgggtca agcaggcttt taaatagatc ttctttgttt
     3421 ttgaagtaat tatagaaatt ccccagcgac agccctgcct tttcggctat ttcacgtatg
     3481 cccgtggccg aatacccttt tgtggcaaaa agctcttttg ccacatcaag aatctgctgc
     3541 cgcttgtcaa tgattgtatg gctcataatt cacccctgaa cgaacgttcg tacttgtcaa
     3601 tatagccata aatttgattt tcgtcaagat atgatgataa ttttttttta aaaaatggga
     3661 tacgttcaaa aaatgtatta tgtggaggtt gcggagagcg ctggaaatgg ggtcagagca
     3721 tatatcctaa actctgtgtt atgagcgctc tccgcatgag taaatttatt ctttgtacat
     3781 ttcttctata atatctgcat attttttctg gataactttg cgtttgattt tgagtgtagg
     3841 cgttaattca cctgtttcct gggtaaattg ctgtggaagc agtgtaaact ttttaatctg
     3901 ttcaaccttg ccaaactcct gattaacctc attaactatt gcctcatatt ttttacgtac
     3961 atcaggatgt ttaaccagtt cgtcattgga tggtgcagat attccttgag tggctgccca
     4021 ttgtgttaat gtttcaaaat ttggcacaat cagggcaacg atgtattttc gtgcatcccc
     4081 aacgatggca acctgctcaa taaagggatg tatcagcaag cgctgttcaa taacctgtgg
     4141 tgcgatattc ttaccgccag ctgtgacgat aatatctttt ttgcggtcag ttatttttag
     4201 atatccttca ctgtcaagga caccaatatc acctgttttt atccatccat cgtcagtaaa
     4261 catttccttg gtggcttcag gtttattgag gtaacctttc atcaactgtg ggcctttggc
     4321 taatatttct ccgtcatcag caatctttat ctgcgtttca gggaatggac atatccagcc
     4381 atcagatttt ataggcttga tttttggcga accgtcaaca gcaaggacag gtgaagtttc
     4441 ggttaatcca tatcctttgc gtacctgaac attcattgcc cagaagaagt catgtatttc
     4501 ttttgccagc ggtgcaccag cggcacccca gcaaataaca cggtcaaggc caagtgcgtt
     4561 tttaagtttt gagaaaatga gtttattggc aattgcatat tttactgcaa gtactggtgg
     4621 tttgcgtttt gctgccataa agtatggtgc tgcctgaagc cctaccgata gtgcccagtt
     4681 aaaaatcttt tgtttctttt caggcatttc tttaactttt gccataacgc cgttgtatat
     4741 tttttcaaaa acacggggaa ccagaatatc gcattgaggg cggatttcct tgagttcttc
     4801 tgcaaagtat tctgtaccac ggctataata aattactgct ccacgctcta acacgctata
     4861 ataatccatg gtacgctcaa ccgaatgaga tagtggaagt aacgataagt gaataaaacc
     4921 ttctggtaaa tcttcaactt tgttaaacgt aagacatata aacatgatat ttttgtgggt
     4981 cagcataacg ccctttgggt cgccagttgt tccggatgta tagattaatg tcattacatc
     5041 gtcgggtttt acgttctttg tacgcttttc tatctcttca atgtgcagat ttttattacc
     5101 agcatttacg gcatccttaa atgtaatcac catatcatca ttgtaattaa tatcatcaca
     5161 cacaataatt ttctttaaat tgggcagttc actttttttg gaaaggacat tatcagcctg
     5221 aaattttgat gaacacacgc acacaacaga tgaagaatct tttagtatgt atgctgcttc
     5281 aggtgcagaa tttgttggat agatggctgc atcaatgcca ccaattgaaa gagtggctaa
     5341 atcggcaatt acccattcag ggcggttttc tgaatagatt gatacaacat ctccaggttt
     5401 caatcccaga tcaatgaaat acactgccag ggctttaacc attgtatcaa cctgattcca
     5461 ggtgaaggat tcccactgtg tgccattatg atggtttaat aagactttat tgccatattt
     5521 ctttacaccg tctttaaaaa aggtccaaaa cagtatgtgt attataatga taatctggca
     5581 gcatatataa cttcaccatt tttttagaat aatagtattg ttgaaagtgt acgaatagta
     5641 tatcatattg tcaattaaaa tatataaatt ttaataaata tacatgaaaa aatgataatt
     5701 caattgtatc aggctatcac atgcaaatgt tagcatgtta gtatgtacat aaaataaggt
     5761 gtgaaaaagc atcacgcaaa gtgtgctgag atcgcaaaaa gcgctgtggg aaggggcagt
     5821 gttatgtcgg aaattacaac acttcgtgtt ataagcatta tctgcgtgaa aaaaaatgta
     5881 ttacgtagag tgtgcggatg ttactgtagg tgctgaggaa aggggtcaga gcatatgttt
     5941 acaatcatta ttttttttta ctttcagtgt gaggaaaaga aggtataaca taattttttt
     6001 aaaaaaatat tgctaaataa tgttcatttt cttaatgtca ttatttgaac atttaatatt
     6061 acaagtaata aaaggaacat tatgaaggta ttgcttataa atccaccata cccttttgag
     6121 gaatcgccat caccaccatt tggacttatg gcactggctg catatcttga agaaaaaaac
     6181 attgaagtaa aaattgagga ttatatagtc aacaggtttt cagaaaaccg tgtacaggac
     6241 gttatgtcat catttacacc ggatgttgtt ggttcaaccg gagttacgat gaacataaag
     6301 aaggcgttag caattctgaa agtgtataaa cagattaatc cagatatcat cacagtactt
     6361 ggaggacctc acgcaacgtt tgatgcacat aatatgttgc ttcaccatcc gtatgttgat
     6421 gtcattgtgc gtggtgaagg ggaattaact tttgaacagt tactatcgca cctgaataat
     6481 aaaaatgcat atgcagaaat tgaaggcatt tcatatcgcg ataatgacaa tactattgtt
     6541 cataatccaa acagggattt tattccagac attaatatac tcccttaccc tgcacggcat
     6601 ttagtggagc tttcaaaata tagagcgctg ggttttccca ttaacatgat gacatcacgg
     6661 ggatgcccgt ttaattgtat tttttgtgta ggcagcaaga tggttggcag gaaggtacgc
     6721 tattttgaaa caaatcgtgt tgttgatgaa tttgctatgc ttgccaccat gaaatttaaa
     6781 caaattaata ttgttgatga cctttttaca tcacataaaa gccgttgtat tgaagtgtgc
     6841 aaggaaatta aacggcgcgg gattaatcat ccgtggactg cgtttgctcg cgttgatacg
     6901 gtaaatattg agctacttga acatatgaaa gatgctggtt gtaccatgct ttgttttggg
     6961 attgaaagtg gtaatcagga aattcttgat aaagttaaga aaaagataac acttgaaaaa
     7021 tgtaaacagg cagttgctat gtgcagacag gttggcatta gccctatgac gtcgtatata
     7081 ctgggattgc caggcgaaac agaagaaacc attaaaaaga ccctggaatt tgcaaaagag
     7141 ttaagcgata attatggatt ccatgtgtta gcaccattcc ctggtactga agtacgggaa
     7201 atgaaagatt attatggtat tgagatttta accaatgact gggatctgta tgatgctaac
     7261 agggctgttt cggttacgcc gtggattact gctgatagga ttaatgacat agttgataga
     7321 ttcaacggca gcataaagac atatatatta tcgttgggtg aaaagcagaa tcatggccag
     7381 aagctttcac acaatgaaca ggaactaatc agcaatatac ggtcatttga aatagtgcgt
     7441 gacatgatat tggataggct tattgaagaa ttcccgggta tagaaaatgg ggttcccgtt
     7501 gatgttaaaa aggaatttgt ggagtacctg tcgcaaaaaa caaaattttc aaaagaagaa
     7561 ttacacaacc aggttgagcg gcttgtagag ttacaatgta ttgattttaa atacagaaat
     7621 agtacaattt atccagtatg gacttaatat gagtgatgga actactatag ttttttaggt
     7681 ttttatttac catctaatag taattaccgt gtttacattt tgcattgtac aatatattta
     7741 taaaatatta catatagaaa aaagtatagc ggaggatata tgaattttga aacaaccaca
     7801 tgtatatcaa aagagcatta tacactatta tgctattatt cagaaaagta taatgtgaag
     7861 ctctcaaaac ttattactgc tctgttacgt tttgttggtg aacatcaaaa agtttatttt
     7921 accacaaaca gacgaattaa atatagggaa agacagggat gtaattggaa acgaattcat
     7981 attatgctta ccccctatga ttatgagata attctggatt tgaagaagta tgggaaaatg
     8041 tctctggcca agatgattga atttagtatt gaaaattatt ttgaaagatt tgttgacagg
     8101 gttcttaaaa aacaaagtac cgataactat ctatatgcaa attatcattt tgaagttaca
     8161 acagaaaata atatattcag ttacagtgtc tattggggat taccaccaaa aattcttcaa
     8221 aagttaacaa aaaccaatca tctattcttc aaaacgtgac cttaattgtt tcttacttta
     8281 ccactttgat catcagtgtt acaccaaagt gcttgaacca tgggctttta ctgtttgctc
     8341 ttttttctag tttgtccgat agcactcgca gccgcttgtg gaagcgtttg gggatgataa
     8401 tatagtgcca tgtccatggt gtgtggaagt tccatggtgg tctaaattca tgtgcggtta
     8461 tttctacagt gttgaaaaga ggcttgcaga ttttatgtat ttcaccaata cggtagaagc
     8521 gcattctgat aggatcttta tacctgaata tgtggatgct tacaagttgc aaaaatgcca
     8581 tcaacagatt ttcaggagaa aaccgcgaag gcactcttat atatgctgtt cctccaggtt
     8641 ttaatatgcg gtgtatttct tttataactt ttttgccgtt ttctttggaa agatgttgta
     8701 ataccagaag tgaatagact gtatcaaagc tacaatcctt aaatggtaat aacgtaccat
     8761 cgcccctgac caatggtgtt ggaagattgt gatggtgtag atgtttctgt gctgccatga
     8821 gcatctggtg agagatatcg agaccaatgg cattgtcagt tatagccctg acaggcttta
     8881 acacacggcc ccaaccacaa cctaaatcca gtattgtttc atcaggtttt acttcaaaag
     8941 gtggtggagc atctaaaagg tttccgtagt aatcggtcag tacagcttca agccccaatg
     9001 tgataaatgg tgcgtcctta atagcgccat catcccacac ttttatataa tcctgcaaaa
     9061 aattcttatc tctgactatc acctgctttt cccctttgtt ttttagcatt gccattattc
     9121 tttttcataa aagctttata cactaccgta tagtcattgg atgcattcca gaataggtac
     9181 ccgtatgtat tggtgttgat gcatgcatta acctgcgcta atatatagtc ttcattataa
     9241 ttgctgacac gccatttaaa tgcctgtaac catgggcgga ttaatagttt aggagctatc
     9301 gcctgtaact ttttgttccc tgttgcaata aagtaatatg gttcatcccc agggtttgca
     9361 tatccatcaa aattgtcatt gaagtgtgaa gggtacagca ttggtgagat tacatcacag
     9421 cttcgtgcca gtagttcaat tttttgccct aactttgata cgtcttcttc tttctgccat
     9481 gcagcaacgc caaaaatgtc gatagaaact gcggtattga gcttatgcaa ttcattttta
     9541 gcttttgcta aaaattcagt tatggtttcc acttttgttt ttttaccaaa atcatacgca
     9601 tactgtgcat ctgacaggtc ccctgcggta gggaaacgta tatagtcaaa ttgtacttca
     9661 tcagcaccca gtgtagcaat ttcgcaggcc agtgctatat tataatccca tacagtctgt
     9721 tttgttgggt caacccataa ttcctttgat ccctgattcc atattccacc cgtgcgtttt
     9781 gactttattg ccatatcagg gttttttgtt gccagaagat ggtcacggaa tacagcaatg
     9841 cgtgcaatga catacaaatc aagttccttg aaatttcgta tgagcgtaac cacatcatca
     9901 acagggcgct tttcatgtgt attcaattct actactatgg gtacatggct tttataattt
     9961 actatgccgc atacatcttt aacatcaaag acaactgcgt ttattcctgc ctgttttaac
    10021 tcagaaagct gccgcatcat tttgctgctt gctaaactgt taccagaata gtagacccct
    10081 tttacatagg gtatttgcgt cattgatttt ttggaaaggt ctatagttat aaacggcaaa
    10141 ggatgtggta tcacaagaac tgaacctgca ccaactgttt tagaagttaa ggcattgagc
    10201 gtcacaattg cattgtgaag atgtgatgcc ctgtaatagt aggtataccc aatcaacctt
    10261 tgtgcaatgt cactaacccg ttcttctttt ggtgctatat agagaagcat attgccacag
    10321 tgtaaaagct catgtggcgc atggtgatag gggcgcttgt aattgaaaaa gcgtgttgga
    10381 tagataatgg aaaccgtgtc aggtgcaaaa agtgaatctt taatggttat ataatatcct
    10441 ttaatataat ttgcaatata tataagcctg tgccactgga agattataac aagaagaata
    10501 ctcagaacaa taacaagctt gacgatttta cccttatatg ccatagtcat actattcact
    10561 ttcaaaagat aatgtcttac tatcacagtg tgtatcacac tgtaaaaaca aggaaataaa
    10621 ttcaatataa aaattattct atggttcaga ttattaaaaa aaatattttc attattgtac
    10681 aaaaggttga tggaattata aagggtattg ctgatttcaa tgcatatatg aaagctattg
    10741 ttcgttcttt ccgttcattg cggtatctta aaattcggtc actgtatacg atagctgtta
    10801 atcagacacg gtttactggc atagatgctt taccactggt ttcatggatt gcgcttttac
    10861 tgggagcaac agttattatt caggcaacaa ccaatttccc caaatatgga attgaaagct
    10921 ttattggcaa tcttttggtt attatcattg cccgagagct tggtccactg gttaccgcat
    10981 taattgtagt agcacgatca ggctctgcca ttgccgcaga aattgccacc cagacacaga
    11041 acagagaaat cctttcactt gaattgatgg gaattgatac tcgaatgtat attatattgc
    11101 cccgtattgt tgcctcattt gctgcaatat ttacattaat tattattttt gatgttattg
    11161 cattttttgg tgggtatctg atagcattaa ccagcgtcta catccccatg ggaacattta
    11221 cgcagacact acttgatgca tttactttca atgatttact ggtaactatc accaagagta
    11281 ttatatatgg cgttctcatt cctcttattt gctgttacta tggattaaaa ccaaagtcaa
    11341 gttttgagat accaattttt gtctccaagg cagttatccg tacattactt attattttta
    11401 tcataaatgc tgtaatttcg gtattattct atttttaatt atggaatgcg aagaaattaa
    11461 acaacttatt gaagtcaaaa atgttactat tgaaggtggt aagcaaaacc tccgtaatgt
    11521 ttcatttaat gtatgcctgg gggaggatgt tgttttcttt ggtgctgagg acagtggtat
    11581 tgattatatt tgcccgctgc ttgcccatgc tctggaggat tacagtggcg aagtgctcta
    11641 taaagagcaa catattaaac atatggactt tgttgaagtc cataatttca ggaagatatt
    11701 tgggtatgta caacgtgggt atgcgctcat cagcaatatg acagtatacg aaaatatagc
    11761 gttgccactc aggtaccaca ctgatttatc cgaaaaagaa attcatgaac tcgtcgattc
    11821 cctcatagtc tatcttcatt tatctcattg cagggattta cgccccatta tgcttaccca
    11881 gtctgagatg ctgcgcactg cttttgcacg ggctattgcg ttagatcctg acatgctctt
    11941 aattgaacac ccccttgaag gacaatgcct gtggaatgca cagatttttt tgcaggcact
    12001 gcagaataga gcttttgcgc caaataaaac tgttatagtt gtcacatatc agccattagt
    12061 gtatattgat attgcaacgc gttttatcat gcttcatgag ggaagcatag tatttgacgg
    12121 aacaaaggaa gaatttcaaa catttcaaaa tgaatatgta aaacaattcg ttgatgtatt
    12181 gccatatgga ccaatgacct tacggtagga gtaagacata tgaaattaga aaagaatgaa
    12241 atacgggttg ctatatttat ccttttacca ctggtaatat tactggtatt cgttgtcctg
    12301 aaattagggt attcctttgc cacatcaacc attgatgtgt atatgaaggt tgataatatt
    12361 tcttcaataa aaaacggcac acaggtaaaa attaaaggct atacggtagg aagagtagtg
    12421 gatattattc cagtgtataa gccaacatta cattttctgg cgctgttgcg tattaagaaa
    12481 gaaattgata tgtatgaaga ctgttcggca gtaattatga atcagaatat cattggtgat
    12541 actgttatag aaattagaaa tcctgttaaa aaggaagcat tgttgcgtcc tggagcagta
    12601 attgaaggtg ttgagtatgt tagcctcgaa acaatagtgc aggatgtgca cgcgcttctg
    12661 gcaacactga caaatactgt tgcagttatt caggatatat cacttgaaag caaaggcaat
    12721 atcaggaacc tggttgccaa tctttcaaaa agtgtagaga ctataagttc ggtattacag
    12781 gattcgcaaa aagatatact ggctattatg gcttcattcc gtgaaactgc aaaaactatg
    12841 aatgaaattt ctattgaact caaaaaacat cctgttaagt ttttgttcaa gggtgaaaaa
    12901 taatacatgc cgtttttctc agttgttatt cctaccttta acagagcgca tctggtttct
    12961 cgggctgttg aatcggtact tgcgcagaaa tttactgact atgaatgtat tgtggttgat
    13021 gacggttcaa cagatgatac tcaacggata ctatcgccct ataaaaaaca catacagtat
    13081 atgtacactc gtcatggtgg tgtaagcaag gcgcgtaact atggtatact acaggcacag
    13141 ggtgagtgga tagcgtttct tgattcagat gatatgtggc tgcccaaaaa gcttgctatc
    13201 cagtatgaat atattcagac acatcctgat gtctatattc atcagactga tgagctgtgg
    13261 atacgaaacg gcagacgcgt caatccaatg aaaaaacatc agaaaaaaga aggatacata
    13321 tttgaagatt gcctgtatct gtgtttgata agcccgtcgg cagtggtagt tcacaggagt
    13381 gtgtttgaac gtgttggcat gtttgatgag cttatgcctg catgtgagga ttatgacctg
    13441 tggttgcgtg ttgcctggca atactacata ggttttattc cacaaaggct tattgtaaaa
    13501 tatggtgggc acagcgacca gctgtcatca gccctgtggg gcatggaccg tttcagggtg
    13561 tatgcgctgt gcaagcttat tgccagctat ggtttgctac ttccagaggc ttacttcaat
    13621 aaagcagttg aggttgcact tcagaaatgt tctgtattat atcagggggc aaaaaagcgc
    13681 tctaacgttg cattaataca aaaagtggaa cgggttgtgc agtggctcac tgttgatagg
    13741 aaaagcacat cagtgtatcc tgacctctta gaagaataat attgtgcgat agtatatggt
    13801 gaaggaatgg ttcttcataa aattctcgct tgtatactat acctttgccc cattgccaca
    13861 cataccacac tgagccacct gcatcggcag gataacttat cataaagata tcatcattac
    13921 cacttacctg agattgacca tagcgtttct tgatgatatc atacggggta acatcttttc
    13981 ctttgatatc aatctcaagt aacttttcaa aatccagaaa tagtgctttg ccgtaagtgt
    14041 taattgccat tgatgttttt acataatgcg gtgccgatag ttcataagca aaactctttt
    14101 gcctggtaat attaattccc accacataat ctttgctgct attgccacag tgtaccagcg
    14161 catactttcc agtatcatca atagcaatac ctttaacaat agttgatgat ggtgaagttc
    14221 ccttataaat aatctgtcct gtattattgc atatataata gcgtccgtcg ataaagccta
    14281 tccctgcaaa agaacctttt tgtgaaaatg ctatcgtggt ggtaaattgt ccgttaagtt
    14341 catcatgggg gtatacattg ccgttaaagt cagcaatacg tgctgagctt ccatcagcag
    14401 tgagtagtat aataatggaa ccgtcaggtg aaatgtatgg gtactcggtt gaaggtagtt
    14461 tccagaaaaa ctcatttgaa gcattataca gggctatttc attgccaact tttttatagg
    14521 ttatataaaa tttaccatcg ctggtaggtt taacaaatgc atcaccagct gtaataaatt
    14581 tgtgaaccaa accactggca ccaatatggt agtagccgct gtcggtggta acagcaaatg
    14641 acattttttc atcattaccg ggtgcagctt ctatagcctg tattttttgc aatgaaccat
    14701 accaaagtgg agtaagcata aatcctgtgt gagtcatgaa aacaggcgaa aatcccagaa
    14761 gcatcccaac cagtaatcca attattgaat atctcatagt ttgtaatcaa tccgttaaaa
    14821 agctttttaa tttctggctg cgggagggca accgcagttt acgaatggct tttttttcaa
    14881 tctgccgaat acgttctttt gaaagtttaa agcgttcacc tatctgctgc aacgacattg
    14941 gttcattacc gtctaaacca taacgcaatt ttaatatctc acgttcggat ggtgttaagg
    15001 tgtcaagtac ttcattgaga gcttctttca atgattcatc aaataccttt ttgtcaggcg
    15061 actctacttt aagatcctca accaggctca atacggtatt atcatcggaa ttgccaatgg
    15121 tggcatccag cgaaacaaaa tcctgggtta tatttcgcaa gtggttgatt tcatcattat
    15181 ccatatcaag ttcttcagca atttcagcag ggcttggttc tcggccgagc ttgctttcaa
    15241 gcgacttatg aaccctgtca attttttgca tatctgcagt tctattcaat ggcagacgta
    15301 tcagtgaagt tttttgtgaa atagccagaa gtattgcctg cctgatccac cacactgcat
    15361 atgaaataaa atggtatcct ctatcggggt caaagcgctc agcagctttg atgagcccca
    15421 tgtttccttc attgattaaa tccagtaaag aaataccact ggtttgatat ttcttggcaa
    15481 ttgatacaac aaaccgtaga ttagctttta ttaattcttc tttagctttc tggtcaccgg
    15541 cacgagcacg gcgtgcaagt gcatcttcct cttcccgtgt caaaagagga attttattaa
    15601 tctgctctaa gtaccatgat atagaagaat cggtaaaatc atcaaagttg gctttaatgg
    15661 cttcattatt attttttgta actttattct gcttttgaag cttgagtttt tttgcttggc
    15721 tttcaggtgc agacattata tttgattttg taagtttttc ctgtttaact ttttttggca
    15781 tacttctccc tgttaatcac taaataagta gtaaaaacta ctatccggtt acattattta
    15841 attcaatgat tattttgaca aggcaaaaaa aaacactaat aattaataaa attgcagctt
    15901 cacgtttaaa gtgcaccatg cagttttctc tgtaaaacct catacaaaga tttccatata
    15961 ttagattttc ctggacgtgc atgtatcatc attgttatac taactattcc ctcatcgatt
    16021 acatgtgcaa aacttttttc tctggaaaaa atatcgatat aacctgttgg tgttttccac
    16081 aatcccccgt tgccaggcac attacgtatc tgcacaataa atcttacagt ccagtatctc
    16141 ctcttatttc ctgtgatagg tacattctgt cacattatca aatggtatgg tttttacaat
    16201 ttatcctgtg aaactttagg ggcatctcac ttactatctc tactatccca cgcataacgg
    16261 tgtctgcatc ctttgaaact atacaccctc ccttggactt tcttttgtcc agacggcaac
    16321 aactccatag tattcttttc acaaggggaa a
//
LOCUS       DAORYL010000070        15765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_3027_length_15765_cov_13.373393, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000070 DAORYL010000000
VERSION     DAORYL010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15765
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3027_length_15765_cov_13.373393"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1902)
                     /locus_tag="PLV81_11690"
     CDS             complement(16..1902)
                     /locus_tag="PLV81_11690"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOM10802.1"
                     /translation="MYKLKIFSCLFILAIGTAVFAQEETPLEDVVISAYKSTITYGQS
                     SSVSIITGKDIEQGGYRFVSDVLQTVPGLTLVQNGTLGGTASVFIRGAKTGNTVVLID
                     GVKVLDPSTIERTFDFGTLTTDSIERIEIIKGPQSVLYGSDATGGIINIVTKRGQSKP
                     QVTVNLYGGSYYTTQLSANISAGDSSFNYSFTAGQLSAQGYSRATSPSTATTPYDDDG
                     MYQRYAMGKLGYTPFTVLSIDAGFAVKETDLDLDDYALIDDANSYENRKEISAYTQLE
                     HTITHFWKHSVNASVSQTIRSYDDFDDDGSGGFADSGADNCYTGTIAHTEWLHTFTLK
                     DINTLNLGISFEKEYSKVQDYLASSKLTNDTYVTGFFAQDHVNVGKMFYLTAGVRADK
                     NNIFGWQQSYIVSPAVVIPTVKTKLKGNWGKGYKAPSLYQIYGDGGVYVDENKDLKPE
                     ENTGYDAGIEQPLFDILTVGIAYFKNNYRNMIDYDSSTWPGKYVNIGRVTTNGWEGSF
                     TLKPAEALTLGGGYTYTKAIDESTDKQLLRRPKHQGYGSVTINIDSFAISLIGIYVGE
                     RMDSGNNTLKNYMKTDIAAQYALTPQLTIQAKAENITNKQYQQAYGYNMPQRSFYAGL
                     KATL"
     regulatory      complement(2041..2180)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2337..2738
                     /locus_tag="PLV81_11695"
     CDS             2337..2738
                     /locus_tag="PLV81_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HOM10803.1"
                     /translation="MQDIRYKQRFHNYSQSFALLQDSLKVQNPTILEKAGIIQFFEIT
                     FELSWKLLKDYCEYIGYDIHSPRDAIKTAYSIGIIDNGQTWLDALMDRNLTVHTYDED
                     IANEVYNKIKNEYYPLLEKLYDTFKDKVCTD"
     gene            2726..3001
                     /locus_tag="PLV81_11700"
     CDS             2726..3001
                     /locus_tag="PLV81_11700"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018664960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOM10804.1"
                     /translation="MYGLTDDELQRIREALITCGVTRCILFGSRAKGSNKPGSDIDIA
                     IDGNETRVSYCLNEELNVPYYFDVINIHKIKNKNLLDHIQRVGVELL"
     gene            3214..4773
                     /locus_tag="PLV81_11705"
     CDS             3214..4773
                     /locus_tag="PLV81_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOM10805.1"
                     /translation="MNLIDAIAAEGRKTANKIRIVVAIILFLVVVSAAPNNPAIVNIS
                     YLITITIFTVISIVNIQSVSSVFYSKIIKFGTVLFEISIPTILKLSHLATAKPHLMIN
                     EGAAFQAYFLFIMLTLFQNDRFLTILTGIVATVEYSVLVIIGIFVMHMPVISGSSEWG
                     HIVIDDEIGKLVMLIGFTAITVAVLRNLQRFAFTALENEKNALTKADQLKEIVNEAKT
                     VNKSLIDVSQNQTEISAVLSDVANDEATMSEELSSMYEEQTSAIELIYKNTIKQTDET
                     KLMKDRIHEFIEIQKNVITVGEQMLNNIVSITDYSKKTEASLNELSHIMDIVTQSGGA
                     INSFLEVIRNITDQINLLSLNASIEAARAGEYGRGFAVVADEIGKLAMATSDNAKEIS
                     SQLARINQDITSSISIMQNTQKALNDMIAIISASKSNINEVRNAIGNQQKAIEDVKRQ
                     ADVLDSLSHEILMAINEQQSSMNESVSSIQKLSDVAQIVADRTMQMNELTIQINAIAQ
                     TLAKVTAAIDN"
     gene            complement(4786..5568)
                     /locus_tag="PLV81_11710"
     CDS             complement(4786..5568)
                     /locus_tag="PLV81_11710"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOM10806.1"
                     /translation="MSLFEFTNIQAGYRKIPVLDIPRCTINTGDFIGIIGPNGAGKST
                     LLKLLARILAPLSGTIAYKKQNITAIPHTIYATQVSSVTDIEHVLPYTVGSFIALGRF
                     PHATVTGLKAHDYKAINEAIQYCNVQHLQQKKLTELSSGELQRVNIARALAQSTECIL
                     LDEPVSHLDIHHAIAIMDILRKLNEHGSTIIAILHDINIASEYCSRIIGVKDGKIFFD
                     GTPQECITYQNIEALFDCVCLVYTNPISGKPYVYPVPEHVKQ"
     gene            complement(5565..6524)
                     /locus_tag="PLV81_11715"
     CDS             complement(5565..6524)
                     /locus_tag="PLV81_11715"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOM10807.1"
                     /translation="MKKFSIIISIILVCTGCLLILAIALSTGASHITLLQAITGTDFV
                     AKQIIMLRFLRVLMAFFVGAALAVSGCIFQALLKNPLAEPYTLGVSGGAAFGASIAII
                     TASGAVSVYAFIGGVCTVVVIFFIAKAMQFHPTAIILAGISLGFVLSSGVLLVFAFAK
                     PDTIHRTLMWLSGDLSMAHYEHITLYCMSILVLTTMTLFFHRHLDIISFGTGFATTHG
                     ISTVHIAILFWIASLATSITVAATGIIGFVGLIVPHAVRSIKANHAFLLPVSAIAGGT
                     TLVLADTIGRSIVMPYEIPSGIITGFFGGIFFLILLLTRRDTV"
     gene            complement(6625..7437)
                     /locus_tag="PLV81_11720"
     CDS             complement(6625..7437)
                     /locus_tag="PLV81_11720"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOM10808.1"
                     /translation="MHTRNNTVIAITCFVIIWAIAHSMHSQAANKSLQQYTRIVSLSP
                     SITAMIQDLGCEHLLVGVTQYHPPLSRAIPIVGTITNPNSEAIIALQPDIILHSKEDS
                     PTQKIDMLHHAGLRLYALPSADSFETICSNYRTIAGILNIKDYAIHKLSEYKKKRHAL
                     IHKINRSALILLSANPFIAVSRHSYISNIFADAGIDNTLYYSKTRYPIVQLEYLLAQK
                     VGMIIVLNNDTLPDTIEKTKNIVHMPYDELYLYTPRHYCDSLQHVVTVLYGK"
     gene            complement(7484..8725)
                     /locus_tag="PLV81_11725"
     CDS             complement(7484..8725)
                     /locus_tag="PLV81_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOM10809.1"
                     /translation="MKQKQKPHNYAIFVARQPIFNRHKELYGYEILFRSGFENFYNAL
                     DADASTSKTLINSFLVIGTDSLTGGRRAFINFTEETLKTNLAYFFPKEVLTIEILETV
                     PITHEIITIAKKLKSQGYKIALDDFVFTDEYRELLPFADIIKVDFLNTTTFERIQIHN
                     ETAHLNIKLLAEKIETYEDFHEALSLGYSYFQGFFFSQPEIIESRDIPVYKLNYLEIL
                     QQIHKEDVDFGKLEEIIKRDVSLSFKLLKFINSAAFGFASQIGSIKHALALLGIREFK
                     NWLSFVVLHSIAQDKPQELIVTSLIRAKFAELLASSSQLKPQSSECFLMGLFSLVDAF
                     FDRDKKEIFQQLPLTDDIKNAILKQQGPYAKLYQFITAYEKGNWEAVNRLSEELQLPA
                     EQIAQSYRKAIEWANTFFVHQ"
     gene            complement(8999..10156)
                     /locus_tag="PLV81_11730"
     CDS             complement(8999..10156)
                     /locus_tag="PLV81_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOM10810.1"
                     /translation="MYDFGLTEEQKLLRQNVREFAEKEILPVAQELDETETFSVELTK
                     KMGSMGLFGIVVPQEYGGAGMDYLSYAIVVEEIARIDGSQAATVAAGNSLGIGPIYYF
                     GNEKQKQEWLPRLCKGEILASFGLTEPEAGSDAGASKTTARLEHGQWVINGSKIFITN
                     STSPMAGVCTVQAITGKRDNGKKEVSCILVPNGTPGFTAKKMTRKMMWRASDTGELYF
                     DDCKVPEENLLGKRGEGFHQMLSTLDNGRLAIAAMGLGGAQGAYELALKYAKQRKQFG
                     QPISTFQAIAFKLAQMATEIEAARNLLYKACKLKDAGLPFSKHAAMAKLYCSEVMGRV
                     VDQAVQIHGGYGLMKEYNVERFYRDYKLLTIGEGTSEIQKIVISRQIGCYE"
     gene            complement(10206..10676)
                     /locus_tag="PLV81_11735"
     CDS             complement(10206..10676)
                     /locus_tag="PLV81_11735"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HOM10811.1"
                     /translation="MSFKETIQSNIQLSYFEGQIPVNYQYTLGVAGNKFFETLKQKDE
                     FIASVCSHCGVKTIYPLIYCEECFNPIDTYVSIGLEGELYSFTKCYRDYQGNTYDEPH
                     VIGLIRFDGIKGGIIHRLNINEKDLKLGIRVKAKLKPTNKRTGGLDDIVCFEKV"
     gene            complement(10690..11193)
                     /locus_tag="PLV81_11740"
     CDS             complement(10690..11193)
                     /locus_tag="PLV81_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HOM10812.1"
                     /translation="MKIENVPVKGLSSDEYEKTLKISWKPTLQYAWDNGIGIGKYLQS
                     LKEGKILAAYCEKCGRIMLPARAFCEMCFRPTDKYLPVKDTGTVNTFAICHVNWDASR
                     LKPDEPRYIPAVIEIDGASPGQGIMHLLGNVEPAKVRIGMKVKAVWKPADKREGSITD
                     ILFFEPY"
     gene            complement(11203..12366)
                     /locus_tag="PLV81_11745"
     CDS             complement(11203..12366)
                     /locus_tag="PLV81_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010923764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HOM10813.1"
                     /translation="MGFRKVAIVGAGITKFVRRAQETGPEMSYLASKIALDQAGLKLK
                     DVEMVTLGTAPDAFDGVHMKGEYLLSGAGGNNKPYIRNFVGGGTGVFAPIHGWMHVAS
                     GACDVCLVVCSEKMSSCHPHPQGAFLTIFDHTTEQPLKPTLIWIFALEMRRYMETYGL
                     TKEDIARVAVKNKNNALDHPSAQVAEAVTIEDILNSEVLAEPVHRLDISPASDGAAAV
                     VLASEDVAKRLTDKPVWIDGVGWNLDTAYWATKDLYYPRYVEKAARMAYEMAGITEPH
                     KEIQVAEPYDPFDYKELHHMEGLLLCERGEAARLTAEGYTARTGNLPTCPSGGALGVG
                     NPIAATGLMKVIEIFLQLREEAGKRQVPGKPRVGVAQAWGDLMQVGTVIVLRR"
     gene            complement(12382..13533)
                     /locus_tag="PLV81_11750"
     CDS             complement(12382..13533)
                     /locus_tag="PLV81_11750"
                     /inference="COORDINATES: protein motif:HMM:NF014820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HOM10814.1"
                     /translation="MKERVAIVGIGVTGFSSVSPSLSFKELTYTAAVKAYHEAGLHPK
                     EIDSFITASEDFMEGYSIFDEYVPDQMGAVMRPVQTVTADFIQALGIGCMMINTGRFR
                     TIAVEAHSKASNVKTLNEVKAFALDPVYIRPLKENADFLAGLEMKRFLEETGNTEEQC
                     AEVIVKNKANALNNPYAAHAASLTIDDVLSSPPISLPLKELDAAKPADGAIMIVLASE
                     KEAKALCKKPIWIRGISWFSGQGNPWCRDFAYADYAQLAAQKAFAMAGIANPLKDLDF
                     AEICDEYSYKELQHLESIGIARKGEAGFLTQTGVTKLGGLLPVNVSGGSLGVGHTYEA
                     SGGMKVVEAVMQLRHEAGIRQIPNAKTGLVQSWRGVPTATGAIALLSNE"
     gene            complement(13721..14263)
                     /locus_tag="PLV81_11755"
     CDS             complement(13721..14263)
                     /locus_tag="PLV81_11755"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOM10815.1"
                     /translation="MDLGSVLRLKRKEKKMTLKAVAEKAGISEGFLSQIENNVNSPSV
                     DTLMNICSAIGCNAAEVIEKAQNQERIAVIRKNDLSDVDIPHVGFATRRFFPPESRRT
                     IDSAILIIEPGKSIPARKGVRNSQEVLCVIKGSLELTYNRDVLTLNQGDTAHFWTLPD
                     NQVVTNKSHYITIALWIGTM"
     gene            complement(14478..15200)
                     /locus_tag="PLV81_11760"
     CDS             complement(14478..15200)
                     /locus_tag="PLV81_11760"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOM10816.1"
                     /translation="MTLEQYIISFLIIFISALVQAITSFGSALVSMSLLPFLFPMNFV
                     TPLVALNGSCITALNFFRLHQHFDPKKIKPLIIGAIPGIPTGIYLLVNLPESIIKTIL
                     ALVIIGYAIYSLINTKSGFTINYRAGYALGFVAGCLGGAFNTDGPPIVIYITSQHWSK
                     DEMNISLTSYFLFSGLTLVTMHTVGGLLTKHVAQFFIMLLPAIFSGVYAGSLVYKTIN
                     HEVFKKIVLILLILVAIVLISK"
     gene            complement(15302..>15765)
                     /locus_tag="PLV81_11765"
     CDS             complement(15302..>15765)
                     /locus_tag="PLV81_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10817.1"
                     /translation="CISLQHIAILEEYAQKLGISLRTFIVYMIMYAAKKEKKKYRAFK
                     RIAYRKRNADNPWKRVHLVLYQSEYEFFLDVKKLWKMSLAHVIEFCVDNILIEFFEYF
                     KVRLAKINSDNYPDNLPSYYENRSYTFDFYRENGIHCLKFYWGPPPEMLSI"
BASE COUNT         4713 a         3017 c         3038 g         4997 t
ORIGIN      
        1 tgcatgacaa caatattaca aagttgcctt taatcctgca taaaagcttc gttgtggcat
       61 attgtatcca tatgcctgct ggtattgttt gttggtgata ttttcagcct ttgcctgtat
      121 ggtaagctgt ggggtcagag catactgtgc agcaatatcg gttttcatgt aattttttaa
      181 tgtattattt cccgaatcca tacgctcacc aacatatata cctataagtg atatggcaaa
      241 tgaatcaata ttaatagtca ctgaaccata gccctgatgt tttggtctgc gtaataactg
      301 tttatcagtg ctttcatcta tggcttttgt atacgtatat ccacctccaa gggtcagagc
      361 ctcagcaggc ttcaatgtga acgatccttc ccagccattg gtagtaaccc tgccaatgtt
      421 aacatatttt cccggccagg tgctggaatc gtagtctatc atattgcgat agttgttttt
      481 aaaataagca ataccaacag taagtatatc aaataatggt tgttcaatgc cagcatcata
      541 tcctgtgttt tcttcgggtt ttaaatcttt attttcatca acatatactc cgccatcacc
      601 gtagatttga tacaggctgg gtgctttgta gcctttgccc cagttgcctt tgagtttggt
      661 ttttactgta ggtataacta cagccggtga tactatgtag ctttgctgcc agccaaagat
      721 gttgttttta tcggctctga cccctgcggt aagataaaac attttaccaa catttacatg
      781 atcctgagca aaaaagccag tgacatacgt atcattggta agttttgatg aagccaggta
      841 atcctgaact tttgaatatt ctttttcaaa actgatgcca agattcagcg tatttatatc
      901 tttcagtgtg aatgtgtgta gccattcagt atgtgcgata gttcctgtgt aacagttatc
      961 cgctccagaa tcagcaaaac caccgctacc gtcatcgtca aaatcatcgt atgagcgtat
     1021 cgtttgagat acacttgcgt ttacgctgtg tttccaaaaa tgtgtaatgg tgtgttcaag
     1081 ctgtgtgtat gcggatatct ctttacggtt ttcgtaggag tttgcatcat ctataagagc
     1141 ataatcgtct aaatcaaggt ctgtttcttt tactgcaaag cctgcatcaa tgcttaatac
     1201 agtaaatggt gtgtaaccaa gtttgcccat tgcatagcgt tgatacatcc catcatcatc
     1261 gtacggggtt gttgctgtgg aaggacttgt tgcccgtgaa tagccttgag cggaaagctg
     1321 cccggcagta aaagaataat tgaatgaact atcgccggct gaaatatttg ctgaaagctg
     1381 tgtggtatag tatgagccac cataaaggtt tacagtaacc tgaggcttgc tctgaccccg
     1441 ctttgtaaca atgttgatga taccgccggt tgcatctgag ccgtacagta cactctgggg
     1501 tcctttgata atctcaatgc gctcaatgga gtcggtggta agggtcccaa aatcaaacgt
     1561 acgttcgata gttgacgggt ccagaacctt cactccatca ataagcacta ctgtgttacc
     1621 ggtctttgca ccgcggataa acacgcttgc agtaccgcct aaggtcccat tttgcacaag
     1681 cgttagtccc ggaaccgttt gcagcacatc gcttacaaac cggtagccgc cctgttcaat
     1741 gtctttgccg gtaataatgc ttacggaaga actttgccca taggtgatgg tgcttttgta
     1801 tgcactgata acaacatctt ccaggggtgt ttcttcctgg gcaaagacgg cggtgcctat
     1861 cgccagaata aacaggcaag aaaagatttt tagtttgtac atgaaaacct cctcaaaagt
     1921 ttggaaattg gtactggaat gaggaggagg gaaaccggaa agctacataa aaaacgcatg
     1981 ctctgaggca tgcggagtgt tagaatcaaa ttgctttgat gctaactggc tcccaatcct
     2041 cgaggaattc cagctacaag gagtgtctgg cttatcccgg agttccagga atcacagttg
     2101 cgggtcagcg tgggatttgc acccaacttc ctcctgcttt acattttttg taaagcaatg
     2161 ctaatagcaa ttgatataag gatttgtgtc aataaaaaat ttatttgatt tttggtattg
     2221 gttgattgtt aaaaaatatt atattaatgc cagcatgata tatttaagat aataattgac
     2281 aaagctatgt aaaatatatt ataagaacat agagttaact ttgacacgat aacagtatgc
     2341 aggatatcag gtataaacag cgatttcata attattctca gtcatttgcg ttattgcagg
     2401 attctttaaa agtccagaat cctactatat tggaaaaagc aggtattatt cagttttttg
     2461 aaataacatt tgagttatcc tggaagcttt taaaagatta ctgtgaatat attggatatg
     2521 atatacattc acctcgtgat gctataaaaa ctgcatattc tattggaata atagataatg
     2581 ggcaaacctg gcttgatgct cttatggata gaaatttaac agtacatacg tatgatgagg
     2641 atatagccaa tgaagtgtat aataaaataa aaaatgaata ttatccgtta ttggaaaaat
     2701 tatatgatac attcaaggat aaggtatgta cggattaaca gatgatgagt tacagcgaat
     2761 acgagaggca ttgattacat gcggtgtaac acgatgtata ttgtttggtt cacgcgcaaa
     2821 aggtagtaat aaaccaggca gcgacataga tattgccatt gatggcaatg aaaccagagt
     2881 aagctattgc cttaatgaag aacttaatgt accgtattat tttgatgtta taaacataca
     2941 caaaattaaa aataagaatc tgttagatca catacagaga gtgggtgttg agttgctgta
     3001 atagcaacac atttctacat attataaaaa ctatatcttt gcaaaataaa aaatggtaag
     3061 tatatatgtt gctgtaattt tatgtgttga cttacaatag cgattgtata ggattgtatt
     3121 taacgagcgt ctgctcgtat tgggattatt tttatcattg cgtctgctca ttacgtccca
     3181 atactatgat catattattc tggagaattg ataatgaatc ttatagatgc tattgctgct
     3241 gaaggcagaa aaactgcaaa taagattcgg attgttgttg caattatttt atttttagtt
     3301 gttgtatcag cagctccaaa caatcctgca attgttaata taagttatct gataaccatt
     3361 actatattta cagttatttc aattgtgaac attcaatcgg tatcttcggt attttactca
     3421 aaaattatta aatttggtac agtacttttt gaaatatcca taccaactat tctcaagctt
     3481 tctcatttgg ctactgcaaa gccacacctg atgattaatg aaggagcagc tttccaggca
     3541 tactttttat ttatcatgtt aacgttgttt cagaatgatc ggtttttaac aatcctcacc
     3601 ggtatcgtgg ctacggttga atacagtgta ctggttatta ttggtatttt tgttatgcac
     3661 atgccggtta tttcaggtag ttcagaatgg gggcatatcg ttattgatga tgaaattggc
     3721 aagttagtta tgctcattgg ctttactgca ataactgttg ctgtattacg gaatttacaa
     3781 cgatttgcat ttactgctct ggaaaatgaa aaaaatgcac taactaaagc tgatcagtta
     3841 aaggagatag ttaatgaagc taaaactgta aacaagtcgc ttattgatgt aagccagaat
     3901 caaactgaaa ttagcgctgt tctttcagac gtggcaaatg atgaagctac aatgagtgaa
     3961 gagctttcat caatgtacga agaacaaacc tcggcaattg aacttattta caaaaatact
     4021 atcaaacaaa cagatgaaac aaaattgatg aaagacagga tccatgaatt cattgagata
     4081 cagaagaatg taataactgt tggcgaacag atgttgaata atattgttag tataactgac
     4141 tattcaaaaa aaacagaagc aagtttaaat gaattatcac atatcatgga tatagtaacc
     4201 caaagcggtg gtgccattaa ttcatttcta gaagttattc gtaacattac tgatcagatc
     4261 aatttgcttt cgttaaatgc aagcattgaa gcagcccggg ctggtgagta tggcaggggt
     4321 tttgcggttg ttgctgatga aattggtaaa ttggcaatgg ctacctcgga taatgcaaag
     4381 gaaatatcat cgcaactggc acgcattaat caggatataa ccagcagtat ttccatcatg
     4441 caaaatacgc agaaggcatt gaatgacatg attgccatta taagtgccag caagtcaaac
     4501 atcaatgagg tgcgtaatgc aataggcaat cagcaaaagg ctatagaaga tgtaaaacgt
     4561 caggctgatg tgcttgattc actttcccat gaaatactca tggcaataaa tgaacagcag
     4621 tcatccatga atgagagtgt gtcatccata cagaaactat cggatgtagc ccagatagtg
     4681 gcagatagaa ccatgcagat gaatgaattg acaattcaga ttaacgcaat tgcacaaaca
     4741 ttggctaaag ttaccgctgc aattgataat taaaaggtca gagatttatt gtttaacgtg
     4801 ctcaggtaca gggtacacgt atggttttcc tgagattgga tttgtataca caagacacac
     4861 gcagtcaaac agcgcttcaa tattctgata ggtaatacac tcttgtggtg ttccatcaaa
     4921 aaatatcttt ccatctttaa cgccaatgat gcggctacag tattcagatg caatgttgat
     4981 atcatgcagt attgcaatga tggtgctgcc gtgttcattg agcttgcgca ggatatccat
     5041 gatggcaata gcatgatgta tatctaaatg cgataccggt tcatccagta gaatacactc
     5101 agtactctga gccagcgcac gggcaatgtt aacacgctgc agttcgcctg aggaaagctc
     5161 ggtaagcttt ttttgttgta agtgctgtac gttacagtac tgtatggcct cattaattgc
     5221 tttgtaatca tgtgctttta aacctgtaac cgtagcatgg ggaaacctgc ctaacgcaat
     5281 aaacgacccc accgtgtagg gaagcacatg ttcaatatca gttactgatg atacctgtgt
     5341 tgcatatatt gtgtgtggta tggctgtaat attttgtttt ttatacgcta tagttcctga
     5401 taagggggct aaaatgcgtg caagcagttt cagcagtgtt gatttaccag cgccgtttgg
     5461 tccaatgatg ccaatgaaat cgccagtgtt aatggtacaa cgcggaatat cgagcacagg
     5521 aatttttctg taaccagcct gtatgttggt gaattcaaat aaactcatac tgtgtctctt
     5581 cgtgttaata gaagaatcag gaagaatatc ccgccaaaga atccagtaat aatgcctgat
     5641 ggaatttcat atggcatgac gatgcttcgg ccaatggtgt cggccagtac cagtgtggtg
     5701 ccgccagcaa ttgctgatac cggcaaaaga aatgcgtggt tggcttttat actgcgcact
     5761 gcatgcggca caatgagccc aacaaagcca atgatgccag ttgcggccac ggtgatgctt
     5821 gttgcaagtg atgctatcca gaaaagaata gctatatgaa ctgtgctgat gccatgggtt
     5881 gtggcaaacc ctgtaccaaa tgatatgata tctaaatgcc gatgaaaaaa aagtgtcatt
     5941 gttgtaagca ccagtatact catgcaatac agtgtgatat gttcatagtg agccattgac
     6001 aggtcgccag agagccacat cagcgttctg tgtatggtat cgggttttgc aaatgcaaat
     6061 acaagcagta cccccgatga aagcacaaag cctaatgata tgccggctaa aataattgct
     6121 gtgggatgaa actgcattgc cttagcaatg aagaaaatca ctaccacagt acacacacca
     6181 ccaataaatg catataccga aactgcgcca gatgcagtaa taatggcaat gcttgcacca
     6241 aacgctgccc cacctgatac acccagtgta tacggttcgg ccagtggatt ttttaacagt
     6301 gcctgaaata tgcagcctga cactgccaga gcagcaccaa cgaaaaatgc catgagtact
     6361 ctaagaaaac gcagcataat tatttgtttt gcaacgaaat cggtgcctgt gattgcctgt
     6421 agcaatgtta tgtgcgatgc acctgttgaa agagctatcg ccagtatgag taagcagcct
     6481 gtacagacca gtataatact tatgattatg gaaaattttt tcatagcttg caattggata
     6541 aaagtatcag tatcaaagct tcattatgtc aacattttaa attggtgcga tagctcataa
     6601 tagataaatt ttgccataaa tatttcactt accatataac acagtaacta cgtgttgcaa
     6661 tgaatcacag taatggcgtg gtgtgtacag atataattca tcatatggca tgtgaacgat
     6721 gttttttgtt ttttcaattg tatcaggtaa tgtgtcattg ttcagtacaa taatcatacc
     6781 aactttttgc gcaagcaaat actccaactg tacaataggg tagcgcgttt tgctgtagta
     6841 caatgtattg tcaatgccag catcggcaaa gatattgctg atgtatgaat gccgtgagac
     6901 agcaataaaa ggattggctg aaagcagaat aagtgcactg cgatttattt tgtgaataag
     6961 agcatggcgc ttttttttat actctgaaag tttgtgaatg gcataatctt ttatattcag
     7021 aatacctgct attgtacgat agttactgca aatagtttca aatgaatctg ccgaaggtag
     7081 agcatacaat ctaaggccag catgatggag catatcaatc ttttgtgttg gtgaatcttc
     7141 ttttgaatgg agtatgatat caggctgtaa tgcaataatt gcttcactgt tgggattggt
     7201 gatagtgccc actataggta tagcccttga aagcggaggg tggtattgcg tgactcctac
     7261 aagtaaatgt tcgcatccta aatcctgtat cattgcggtt atggatggcg acagtgagac
     7321 aatgcgggtg tactgctgca aggatttgtt ggcagcctgt gaatgcatgc tatgagctat
     7381 cgcccagatt ataacaaaac aggtgattgc aataactgtg ttgtttctgg tatgcatgta
     7441 tcatggtgat aatagtctgg aaatgtctgt caaggaatat gttctactga tgcacaaaaa
     7501 atgtgttagc ccattcaatt gctttacggt atgattgcgc tatctgctca gctggtaact
     7561 gaagctcttc tgataatctg ttcactgctt cccagttacc tttttcatag gcagtaataa
     7621 attgataaag ctttgcatag ggcccctgtt gctttaaaat tgcatttttt atatcatcag
     7681 taagaggtag ttgctgaaaa atttcttttt tatccctgtc aaaaaatgca tcaaccagtg
     7741 aaaataagcc catcaggaaa cattcggacg attgtggttt taactgtgat gagctggcta
     7801 aaagttcagc aaattttgca cgtattaatg atgttacaat gagttcctgt ggtttatcct
     7861 gtgctatgct gtgaagcacc acaaatgata gccagttttt gaattcacgt atacccagta
     7921 gtgccagtgc gtgtttaatt gaaccaatct gactggcaaa tccaaacgca gcagaattga
     7981 tgaatttaag cagtttaaag cttaatgata catcacgctt tattatctct tctaatttgc
     8041 caaaatcaac atcttcctta tggatttgct gtaatatttc taagtagttg agtttataga
     8101 caggaatatc tcttgattct ataatctctg gctggctgaa gaaaaatccc tgaaagtatg
     8161 agtatcccag tgaaagtgct tcgtgaaaat cttcataggt ttcaatcttt tcagccagta
     8221 gtttgatgtt gagatgtgct gtttcattat gtatctgaat gcgctcaaag gtagtcgtgt
     8281 ttaaaaaatc aacttttatg atatccgcaa aagggagtaa ttcacggtat tcatcagtaa
     8341 atacaaaatc atcaagcgcg attttatacc cctggctttt tagctttttt gcaatagtga
     8401 tgatttcatg tgtaatggga actgtttcaa gtatttcaat tgtcaggact tcttttggga
     8461 aaaaataggc aaggtttgtt tttaaggttt cttcagtaaa attaatgaat gctcttctgc
     8521 cacctgtcag tgaatcggtt ccaatgacta aaaagctatt aataagggtt tttgatgttg
     8581 atgcatcagc atcaagtgca ttataaaaat tttcaaagcc gctacggaat aatatttcat
     8641 agccatataa ttctttatgc ctgttaaata ttggttgccg tgcaacgaaa atggcataat
     8701 tatgtggctt ttgtttttgt ttcataataa aacagtttgc tttaagttta tatcccagtc
     8761 tcttatgaga gggatatata aatctaatcc aattatagta taaaatttgt aataaaaaat
     8821 ttacactatt aatgatatat tacaacacta ttattggcaa gtattttata ctaatggtaa
     8881 aatttttatg gatatttttc ttgtcaagaa tttttacacg gtttttcctg aataaggatt
     8941 caggatctca ggtttttcaa acaaattcca tgattggcat gaaaggttgg tgccttattt
     9001 actcataaca gccgatttgc cgtgatatta ctattttctg tatttctgat gtcccttcac
     9061 caatggttaa cagcttgtaa tccctgtaga agcgctcaac attatattct ttcataagtc
     9121 catacccgcc atgaatttgc actgcctggt ctaccacccg ccccatgacc tcggagcagt
     9181 atagttttgc catggcggca tgctttgaga aggggaggcc agcgtctttt aacttgcagg
     9241 ctttatacaa taagtttcgt gcggcttcaa tttcagttgc catctgggca agcttaaatg
     9301 ctattgcctg gaaggttgaa attggttgcc caaattgttt gcgttgttta gcatatttta
     9361 atgctaactc gtaagctccc tgtgcaccac caaggcccat ggcggctatt gcaagccttc
     9421 cattatcaag tgtcgatagc atctgatgaa atccttctcc acgtttgccc agtagatttt
     9481 cctccggcac cttgcagtca tcaaagtaaa gttcccctgt gtcagatgca cgccacatca
     9541 tttttcgggt catctttttt gctgtaaaac ctggagtgcc atttggcacc agtatacacg
     9601 atacttcttt tttcccatta tctcgtttgc cagtgattgc ctgaactgtg cacacgcctg
     9661 ccattggtga tgttgagttt gtgataaata ttttgctgcc gtttataacc cactgcccat
     9721 gttctaaacg ggcagtggtt ttgcttgctc ctgcgtcaga accggcttcc ggttcagtaa
     9781 gaccaaatga tgccagtatt tcgcctttgc acagacgagg cagccactct tgcttctgct
     9841 tctcattgcc aaaataatat atgggaccaa tccctaagga attgccagca gctactgttg
     9901 cagcctgtga accatcgatg cgagctattt cttcaacaac tatcgcatat gataaataat
     9961 ccatgcctgc accaccatat tcctgtggca cgacaatccc aaaaaggccc atggaaccca
    10021 ttttttttgt cagctctact gaaaaggttt ccgtttcatc aagttcctga gccacgggta
    10081 aaatttcttt ttcagcaaat tctcgcacat tctggcgaag aagcttttgt tcctcggtta
    10141 acccaaaatc atacatgggg aacctctttt attattttta tggtaacatt atgttgtttt
    10201 ggtatttata ctttttcaaa gcacacaata tcatcaagac caccagtgcg tttatttgta
    10261 ggctttaatt ttgcttttac cctgattcct aattttaaat ctttttcatt aatatttaaa
    10321 cggtgtatta ttccgccttt aattccatca aatcgaatta agcctataac atgtggttca
    10381 tcgtatgtat taccctgata atctctataa catttggtaa atgaatacaa ttcgccctct
    10441 aatccgattg atacatatgt atcaattgga ttaaagcact cttcacaata aatcaatggg
    10501 tatatagttt ttacgccaca atgtgaacat actgatgcaa taaattcgtc tttttgttta
    10561 agtgtttcaa aaaatttatt tccagctact cccagagtat actgataatt aactggtatc
    10621 tggccttcaa aataacttaa ttgtatattt gattgaatag tttctttaaa tgacatggca
    10681 ttcctccttt tagtatggtt caaagaaaag aatatcagtt attgaacctt ctcttttatc
    10741 tgcaggtttc catactgcct taaccttcat accaatacgc acttttgctg gttcaacatt
    10801 gccaagtaag tgcataattc cctgacctgg tgaagcacca tctatctcaa taactgcagg
    10861 gatgtagcgc ggttcatcag gtttaagacg gctggcatcc cagttgacat ggcatattgc
    10921 aaatgtattt acagtgccag tatctttaac aggtaaatat ttatcagttg gcctaaagca
    10981 catttcgcaa aaagcacggg cgggcaacat tatgcggccg catttttcac aatatgcagc
    11041 taaaattttt ccttctttta acgactgcag atatttgcca atgccaattc cattatccca
    11101 agcatattgt aaagttggtt tccatgatat tttaagagtt ttttcatatt catcacttga
    11161 aagacctttt actggaacat tttctatttt catatttgcc tcctatcgtc gtaatactat
    11221 aactgtgcca acctgcataa gatcgcccca tgcttgggca actcctacac gtggctttcc
    11281 agggacctga cgtttgcctg cttcttcacg caattgtagg aaaatctcga tgaccttcat
    11341 caatccagtt gcagcaattg gattgcctac tcccagtgcg ccaccagaag gacatgtggg
    11401 aagatttcct gttcgtgctg tatacccttc ggctgtaagt ctggcagctt cgccacgctc
    11461 acacaataac aagccttcca tatggtgaag ctctttatag tcaaaggggt catagggttc
    11521 cgctacctgt atttctttgt gtggctctgt aatgcctgcc atctcgtatg ccatgcgtgc
    11581 tgctttttca acatatcgag gataataaag gtcttttgtt gcccaatatg ctgtatctaa
    11641 attccaacct acgccatcaa tccataccgg tttatcagtc aatcgctttg ctacgtcttc
    11701 gcttgccaaa actacagctg cagcaccatc tgatgccggg ctaatatcta aacggtgaac
    11761 gggctcagct aaaacttcag aatttaaaat atcttcaatg gttactgctt cggctacttg
    11821 tgctgatgga tggtcaagag cattgttttt attttttact gcaacacgag caatatcttc
    11881 tttggttaat ccatatgttt ccatgtaacg acgcatttct aatgcaaaga tccaaatcaa
    11941 tgttggtttt aacggttgtt cggttgtatg gtcaaaaata gttaaaaatg ctccttgagg
    12001 atgcggatgg caggatgaca ttttttcaga acatacaacc aggcatacat cacatgcacc
    12061 agatgcaaca tgcatccagc cgtgaattgg tgcaaaaact cctgtgccac ctcctacaaa
    12121 atttcgtata tatggtttat tattgccacc agcaccgctc aaaagatact cacctttcat
    12181 atgaacccca tcaaacgcat caggtgctgt gcctaatgtg accatttcta catctttaag
    12241 ttttaatcca gcctgatcca gtgctatctt tgaagctagg tatgacattt cagggccggt
    12301 ttcctgtgct ctgcgtacaa acttagttat gcctgcgccg acaattgcaa cttttctaaa
    12361 tcccatatac accctcctcc tttattcatt tgataataag gcgatagctc cagtggcggt
    12421 tggaacaccc cgccaagact gaacaagacc agtctttgca ttaggtattt ggcggattcc
    12481 ggcttcatgc ctcaattgca taactgcttc aacgactttc attccgccac tagcttcgta
    12541 tgtatggcca acacccaatg aaccccctga aacattaacg ggtaatagtc ctcctaattt
    12601 tgtaacacct gtttgtgtta gaaaaccggc ttcacctttt cgtgcaatgc caattgattc
    12661 taaatgttgt agttctttat atgaatattc atcgcatatt tcagcaaaat ccaggtcttt
    12721 caaaggatta gctatacctg ccattgcaaa agctttttgt gcagctaatt gtgcataatc
    12781 agcataagca aaatctcgac accatggatt accctgccct gaaaaccaac ttataccgcg
    12841 aatccaaatt ggttttttac acaatgcttt tgcttccttt tctgatgcca gtacaatcat
    12901 aattgcacca tcagcaggct ttgcagcatc cagttcttta agaggaagcg atatgggtgg
    12961 tgatgataga acgtcatcaa ttgttaggct tgcagcatgt gctgcataag gattgttaag
    13021 agcattagct ttatttttta ctataacctc tgcacattgt tcctcggtgt ttcctgtttc
    13081 ttcaagaaaa cgcttcattt ctaatccagc taaaaagtca gcattttctt taagaggtct
    13141 gatatataca ggatcaaggg caaatgcttt aacttcattc aacgtcttta catttgaagc
    13201 tttgctatgt gcttcaactg caattgtcct gaatctacca gtattgatca tcatacatcc
    13261 tatgcctaaa gcctgaataa aatctgcagt aactgtttgt acaggacgca taactgcacc
    13321 catctggtca ggaacgtatt catcaaagat gctataccct tccatgaaat cttcagaagc
    13381 tgtgataaat gaatcaattt cctttggatg taacccagct tcatgatagg ctttgactgc
    13441 ggcagtatag gtaagctctt taaaagatag ggaaggactg acagatgaaa aaccagtcac
    13501 tccaataccc acaattgcta ctcgctcttt cataatactt tcctcctcca atatttaaag
    13561 aatggcaaaa tcctgaaata tatattcagg attataatag acataacaaa acaaaaataa
    13621 tcaaagatct cattacattt gattcgataa cattgttata gaaaaaaatg ataagtcaat
    13681 aaatggtcgg aatatttaat agtaaacgaa cgagagtttg ttacatagtg cctatccaaa
    13741 gggcaatggt aatatagtgc gatttattgg taactacttg attatctggt aatgtccaga
    13801 aatgtgctgt gtctccttga ttcagagtca gaacatctct attatacgtt aattccagag
    13861 aaccttttat aacacaaagg acttcctggc tgttgcgaac ccctttacgg gcaggaatac
    13921 ttttgcccgg ttcaataatt aatattgcac tatctattgt cctccgtgat tctggaggga
    13981 aaaatcgtct tgttgcaaat ccaacatgtg gtatatcaac atcagaaagg tcatttttcc
    14041 tgataactgc aatgcgttcc tggttttgtg ctttttcaat gacttctgca gcattacaac
    14101 ctatagcgct gcatatattc attagagtat ctaccgatgg tgaattaaca ttattttcaa
    14161 tctggcttaa aaatccctca gagatcccag ccttttcagc tacagccttc aatgtcatct
    14221 tcttttcctt gcgtttaagc ctgagcactg aacctaaatc catagtttgc tgctaccctg
    14281 gttaatattc ataaataaaa atataagtta tttgtaaata tttactattt attaatatta
    14341 tattaatgtc aagctatttt tttaaaaaat tggtgtgaaa aatattatta ctaatacgtt
    14401 gaattattgt gttatcacta gtaagtgtta ttttttttca ttaacttaat taaatgaggt
    14461 ttagtatttt attatcttta tttactaata agaactatcg ccactaaaat aagaaggatt
    14521 aatactattt ttttaaatac ttcatgatta atagttttat aaaccaagct gccggcgtat
    14581 acacctgaaa aaatggctgg caaaagcata ataaaaaatt gtgcaacgtg ctttgttaac
    14641 agccccccaa cggtatgcat agtaacaagt gttaatcctg agaaaagaaa atatgatgta
    14701 agtgagatgt tcatttcatc ctttgaccag tgctgtgaag ttatgtagat cactattggt
    14761 ggaccatcag tattaaatgc accacctaaa cagcctgcaa caaaacccag tgcataccct
    14821 gctctgtaat taatagtaaa tcctgattta gtatttatga gagaatatat ggcatatcca
    14881 ataattacca gagcaaggat tgttttaatg atgctttcag gcagattaac aagcaaatat
    14941 atgcctgttg gtataccagg aattgctcca ataatgagtg gcttaatttt tttggggtca
    15001 aaatgctggt ggagcctgaa aaaattaaga gcagtgatac atgaaccatt aagtgcaacc
    15061 aggggtgtta caaagttcat gggaaaaaga aatggcaaaa gagacatgga tacaagagct
    15121 gaaccaaaac tggttattgc ctgtactaat gcagatatga atataattaa gaaggaaata
    15181 atgtattgct caagtgtcat aatgataaat acgtcaacta tggaatttgt gccaaataaa
    15241 aaaagtatac aacaattata tcaataacca aatatttata tgcaaaaagt ggatgaaaaa
    15301 attatatgct tagcatttcg ggaggaggtc cccagtagaa ttttaagcaa tggatgccgt
    15361 tttcacggta aaaatcaaag gtgtatgacc tgttttcgta gtaacttggt aaattatcag
    15421 gatagttatc ggaatttatc tttgccaatc gtaccttgaa atattcaaaa aattcaatca
    15481 gtatattatc cacacaaaac tcaataacat gcgctagcga catcttccac agcttcttca
    15541 catccaggaa aaactcatac tcagattggt acaacaccag atgtacccgt ttccagggat
    15601 tatcagcatt gcgtttgcgg taggctatgc gtttaaatgc tctgtatttc tttttttctt
    15661 tcttagcagc gtacataatc atataaacaa taaaagtacg aagagaaatg cctaattttt
    15721 gcgcatattc ttcaaggatt gcaatatgct gtagagaaat acagg
//
LOCUS       DAORYL010000071        15727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_3028_length_15727_cov_12.779416, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000071 DAORYL010000000
VERSION     DAORYL010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15727
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3028_length_15727_cov_12.779416"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(280..1353)
                     /locus_tag="PLV81_11770"
     CDS             complement(280..1353)
                     /locus_tag="PLV81_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014955562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOM10818.1"
                     /translation="MKQILIILLIVFFSCSTSDKQFSEEQWHNHVLHTDTAFLYAPHY
                     KNGEFFNPWMPMKDRSFFHFLKWKFSNNSYSYTEEEKSYLPQVIPNSLERIHSLGNSD
                     FIMWVGHNTFLIRIDSIYFIIDPIFSERALLPKRLTPPGISVNELKQLTSEIHVIITH
                     NHYDHLDEDSIKQLPKQSVFYVPSGLKNYFISLGRNNVVELDWWQEFTMNKNFKIVSL
                     PAQHWSKRVTQSTNSTLWASYMLITPKLTIYIAGDSGYFIGYREYGKKFLHIDYALLP
                     TTAYHPRWFMHYAHMDVHEALMAFEDLGAHYFIPTQWGTFALGEEPPGFPALDLKRKI
                     AEKGLDQKRFLIMDIGEIKILKK"
     gene            complement(1382..1672)
                     /locus_tag="PLV81_11775"
     CDS             complement(1382..1672)
                     /locus_tag="PLV81_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10819.1"
                     /translation="MKSTKRTYTIYCTKPLNEKQIANIQRLLGVDSIHYNHPKCTINY
                     DLQFIDRQLIIRHLKHEKVECKKSLLHKIANALEIFSEKNIIKNNRLLTFYN"
     gene            1794..2735
                     /locus_tag="PLV81_11780"
     CDS             1794..2735
                     /locus_tag="PLV81_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10820.1"
                     /translation="MSIIDYFEANDFNDESLTMIKGNRKIYVIFSTFGEREAELIYQK
                     IRLIETLACIDGIFVSHRRVDDDKEELTEKRANEASSKAKVIVCNTINVPDMGLEKGK
                     GADMRRALYYLITGYKADIHNTVVVFLDADVLAQYFGTHFVLGLAGAVLQGYDFAKAS
                     FWRQMGRVKKYVAQPLFSAIAHRNLDPLREFAYPLSGEVAGTLSFFTKVSFWQMYGVE
                     TGINIDAVLSNFKVADVNLGLYDHEHHGDVNIQKMSFGIIRTFLLQLIDYGYIELKNG
                     AEISDIFQAQYIDEHGRRQSLEFNLSEKKYFPLCTII"
     gene            3057..3815
                     /locus_tag="PLV81_11785"
     CDS             3057..3815
                     /locus_tag="PLV81_11785"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOM10821.1"
                     /translation="MSYDFIKSRLHLYAVLQNIEDLVQYDEEAKKLIKDWNVVIKFSV
                     KGSGNVYLIFENGKCTVTDSSEKKPDIVLFFTSPAHLNKMFDGKANPIPLKGITKLGF
                     LTKEFPKLTERLEYYLKPDDQKLKDSRFVTINTVMTLNTALFALKELLLFDPIGKHIA
                     KKIKGSMVMMNVDKGPAVYIEQKNNGFEVHKGYHEQPLAVLQFKSMKIANDFLNGKMD
                     AFSAIASGAVATKGHIEWLDAVSPVLDRIALYLA"
     gene            3839..5140
                     /locus_tag="PLV81_11790"
     CDS             3839..5140
                     /locus_tag="PLV81_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOM10822.1"
                     /translation="MKLEAKGAPQSSAAKIKSKTYTYSNSHKLFQRALKVIPCGIPGH
                     FSPAPFVPPTMYPFYADRAKGSRFWDVDGNEFIDYMCAYGPMVLGYNNPKVESAASQQ
                     RQKGDCMTAPGKVMVELAEMLTTTIAGADWAFFAKNGGDTTNLAIMIARAATGRKKIV
                     MIKGGYHGVAPWMQGYGHYGVIEEDIANIIGIEWNNVQQFEEAIAANPDGIAGFIATP
                     YHHPTFADSEFPAEGYWQRIRELCTKHGIVLIIDDVRCGFRLDMRGSGAYFGFKPDLA
                     CYCKAIANGYPISALVGIDALRTVAAKVFYTGSYWYQAVPMAAALATLKELKRINGPV
                     LMNKIGKKLFDGMVKICGQYGYDLKVTGAYSMPYVRITNDPSNMLHQEWCAECTARGA
                     YFTSHHNWFVSAAHTEKDIQKTWNIVDDVCKLLKKKYGEKY"
     gene            5234..6076
                     /locus_tag="PLV81_11795"
     CDS             5234..6076
                     /locus_tag="PLV81_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOM10823.1"
                     /translation="MPLSQQELKQLNEKACQIRKDTIDTVYWAGGGHVGGSLSFTEIM
                     VILYYKYLNIDPKNPQWPDRDRVVLSKGHAGVGYAPILADKGYFPKELLKEYNHTGSI
                     LGMHLDGNKVPGVDVSTGSLGHGLPIAVGLALGARLQNKSWLTYCIMGDGECDEGSVW
                     EAAMAAAHYKVTNLVTIVDRNRMMIDGPTEEVMRLEPFADKWKAFGWIVKEVDGHDFN
                     QLADAIEYAQKEDKAPVVIIAHTIKGKCVDFMEDKYEWHYGGLDSEKVELAKACIDKH
                     YCEM"
     gene            6092..7105
                     /locus_tag="PLV81_11800"
     CDS             6092..7105
                     /locus_tag="PLV81_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOM10824.1"
                     /translation="MAADGLTYTIVDTANMSTAEIYGKVLCDLAEKHNDIVGLSADLA
                     KSTKIGQFAAKFPDRFFNAGIAEQNMFGIAAGLAKSGLTPFVSTFAVFASLRAAEQVR
                     TDICYPKLNVKIIATHAGTSFGQAGTTHHCTEDLAVMRSFANMVVIAPADGIETANAV
                     IAAYEYKGPVYIRINRGFDPFLYEKSDYGFQIGKAVQLKDGTDITIIACGAAAYNAYH
                     AAGILEKEDGLSVRVLNMHTIKPIDEEAILKAVMETRRIVTFEDHNIYGGLGSAVAEV
                     IVKSGKACAFESVGIPDEYAIIGLPEDLQNYYGFDQNKIVEKVRQIMGKDFEQTEDWE
                     DEV"
     gene            7107..7883
                     /locus_tag="PLV81_11805"
     CDS             7107..7883
                     /locus_tag="PLV81_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10825.1"
                     /translation="MATQNELFASTIFLKAALPLTKVILEYRPKMAAKYKGLNGVVQF
                     IAKDEAGDIGAYLQFTDGVLDVKYGIHEKPDIVFTFKDVKTMNAFFAGKPAVPSIKGL
                     TKFKLLMKAVNLLLALKIIGRKTPPKKAEDRELMVRLMLYLLPLGISQLNKAGHPEIS
                     KFCKKSPDRTFAWSVNGTDIASYLRMKAGNSKAGRGKYTRSKPFVEFKFDSVDGALGI
                     LLQTTDMIEASINRILVIEGSPEYGKEIGSFMNTVGQLAS"
     gene            7991..8503
                     /locus_tag="PLV81_11810"
     CDS             7991..8503
                     /locus_tag="PLV81_11810"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOM10826.1"
                     /translation="MKVKSLSHVGITVTNFEKAVKWYWEMFRFPLVAEETMDEKQVEA
                     LYSLYGLKNTKLRLGFLRTPGGAVLEIFEFTPAIPSEKVCWNKIGLTHITLDVRNVKK
                     WYKKLKDKGVTFLCEPQKTGPNEWVFMQDMDGNLIELIDLKFNYIALRYLGAIVAPLL
                     RRLQFGKYYK"
     gene            8746..9903
                     /locus_tag="PLV81_11815"
     CDS             8746..9903
                     /locus_tag="PLV81_11815"
                     /inference="COORDINATES: protein motif:HMM:NF019404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM43 family protein"
                     /protein_id="HOM10827.1"
                     /translation="MGDKETYTEVTKTSWLQRLGGSIKSVGVGFLLFIAAFPVLWKNE
                     GCAVKVAQGLTQGAAEVISLPEPKVDLANNGKLIHFYGDAITDEVLTDPLLGISEKAI
                     RIARSVQMYQWKEITKSKTEKKLGGSTETTTEYTYEKVWSDNVINSDSFKYTQGHSNP
                     KAMAIPSQTFQAKNVKVGDFVLNDELISQIQGGEAIAVDASKVAKEYAGLAKQLGNEI
                     YIGNDPNNPQIGDLRISCQVVRAPQKVSVIAQQQGNYLSPFLTKTKTTIQMLHTGIVS
                     ADLMFQEAQESNTIRTWLVRLLGFGLMFFGLMMILKPLVTLGDVVPFIGSILHFGTGV
                     VSFIIAFSLSLIVIAIAWIAARPLLAIILLIIGIGAFITLKVLSTKKTASA"
     gene            10069..14250
                     /locus_tag="PLV81_11820"
     CDS             10069..14250
                     /locus_tag="PLV81_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOM10828.1"
                     /translation="MEKATDSGVTYFIGIDAGSTTLSVVLMDKNKNIIDTYYAYHHGK
                     IKETIEQYLQKKQITKVCGIAITSCTPQVLNASIVNSQVAYVQAAKEYMPHVRTLVIV
                     GGEKFGVIEFDEYGNYSQYRGNTSCAAGTGGFLDQQARRLQLEGSHQLSELALKNYGD
                     IPKIASRCAVFAKTDLIHVQQEGYSIYEICDGLCSGLARNIADTLFASKKPLEPIAVV
                     GGVSLNKAVIKHFESIINTKVLVPELSHLYGSLGAALWAVSNSVSEQIAIEFTHANEQ
                     KEYHYNNLDIVLSQYPEFAGIESYMFTSRHDVEVDIYTPFMPHTEAFIGIDIGSTSTK
                     MAVTDSSGNMLAGFYTRTAGKPLEAVQSLFEALDSYVQRKGVTLTIKEAATTGSGRKF
                     IGTLIGADVILDEISAHAKAAVALNPQVDTIIEIGGQDSKFTMLKNGNVVFSQMNNVC
                     AAGTGSFIEEQAYKLGCLLSEYADRALGKRSPVASDRCTVFMERDINYLMSKGYSTEE
                     LLATVLHSVVENYLSKVSGEAKIGNCICFQGATAKNRALVAAFEQRLQKPIFVSKYCH
                     LTGALGCALEVMETYSGKTKFRGLDLYKKDIPIKNEVCELCTNHCKLKVATIDNEIVA
                     FGFLCGRDYHYKKFVSKTKGIFDFTKERKKILSTSTKDKTATVGIPAALHLVDELDLW
                     IDFFSRLGVSVTTSMDCPDPVATGKRLAQAEFCAPVAAFHGHIAYLVDKCDYIFAPFY
                     LEKDAPQEARRQYCYYTQFAPALGHLLPDAFIKLLSPVLNYEIQPLQSRMALFNACKK
                     MNINANYLEIMMAFEQALAAHKEKNKKLLKVGTQFLKSHNGMAVVLLGRPYNALLDSM
                     NKSIPSMFVNLGIPVLYQDMIDYSSKDVEDINDLLTAFHWHYASKIIETAHVISRKDG
                     LYPVLVTSFKCAPDSFVMEYFKRIMDKAGKPYLILQLDEHDSTVGYETRIEAAIRSFK
                     NHYASKGVPAKQVRVVPAITRESLQGKTVLLPNWDIMAARLLRANLQREGIDAHILYE
                     DDTLIAKGTRSNTGQCIPLNIIAYEYIDYVEKHNLNPAKTILWMADSEISCNIRMYPY
                     FIKSIFDSYGKGFEETQVFVGDISFREVSVKAAIYTYFAFMFSGLLRRAGCHFRPYEC
                     IPGSTDKAVEHTMQEFEQAFRDEIEWSDALGNMVQRFKTIKVKRTNRPKVAIFGDLYV
                     RDNDVMNQNLIAFIEKHGGEVITTPYNYYAKMIANPYFKKWFREGKYKAIVTGKTLLT
                     AVELMEKKYYSYFETLLNEPNPVYTVDFEKLLSQFGVTMYHTGESFDNLIKIFSLLQH
                     YPDIKLFVQTSPAFCCPSLITEAMAHKIYEVTGVPVVSLTYDGTGSFKNDAVVPYLTL
                     LRDTVVQKEAAKKLS"
     gene            complement(14277..14534)
                     /locus_tag="PLV81_11825"
     CDS             complement(14277..14534)
                     /locus_tag="PLV81_11825"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOM10829.1"
                     /translation="MFAITVTSSVKLAGVLIVFALLLSPAMIALSLHVKHPLIIAWIA
                     GTIINIIAICLSYTLDLPTGYTLVALHTITAMCVSFVSVKA"
     gene            complement(14576..15634)
                     /locus_tag="PLV81_11830"
     CDS             complement(14576..15634)
                     /locus_tag="PLV81_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOM10830.1"
                     /translation="MEDIYRALQKRLDMYSLGFPETQSRIEITILKKLFSEEDAKLFL
                     ELSPMLETAESIAKRTGRQQEELTKHLEDMVKRGLLFKKTKDGVAKYGAIPFIHGVFE
                     FQVKRLSKDLAMLLEQYYEEGLSKAVAKSANMFLRPIPVNESIETKSTVAPFEDAVAI
                     IKQASVIAVADCICRKEKKIIGKGCDKPMETCLMFGSMAEYYIENKLGRKISADEAIA
                     IVTEAQKHGMVTQPGTAQNPAGMCSCCGDCCAVLLSIKRFPKPAEMVFSNYYAQTNDN
                     CTSCGNCVDMCQMDAIEITDIAHVNVDRCIGCGLCVMACTNDAVKLVQKEQSKKVPPK
                     TSGEQMMEMAKQRGVLPK"
BASE COUNT         5008 a         2557 c         3326 g         4836 t
ORIGIN      
        1 aatatactcg tgatgaattt ttcagaataa cgaaagttga attaattgat gagtttgatt
       61 cagtgtcaaa agaattttat gaaaaatatg ggaacactat aaaattgaaa aaatcgttat
      121 taaaggctat gtaaaaaata ataatcaatg tattaataaa aagcttctgg atgaaatatt
      181 ccagaagctt tttatcaaaa taaaattggt taatggaaag aaatttgttt ggacctcagt
      241 aaattttatc tttaaaaaga gagaattggt taacaggttt tattttttta atattttaat
      301 ttctccaata tccattatta aaaaccgttt ttgatccaac cctttttcag caatttttct
      361 tttaagatca agagccggaa aacccggagg ctcttcacca agtgcaaatg ttccccactg
      421 cgttggtatg aaatagtgtg cccccaaatc ttcaaatgcc atgagtgcct catgtacatc
      481 catatgggca tagtgcataa accaccgcgg gtgataggca gttgtgggca ataatgcata
      541 atcaatatga agaaattttt tcccatattc acgataacca ataaaataac cactatcgcc
      601 tgcaatatat atagtcaatt tgggagtaat cagcatatag cttgcccata gtgtactatt
      661 ggtagattgt gtaacacgtt tggaccagtg ctgtgcagga agggaaacga ttttaaaatt
      721 tttattcatt gtaaattcct gccaccagtc cagttctaca acattattcc ttcccaatga
      781 aataaaataa ttcttcagac cagatggcac atagaacact gactgtttgg gtaattgctt
      841 tatactatct tcatccagat gatcataatg gttgtgggtt attatgacat gtatttcact
      901 tgttaattgt ttgagttcat taacagatat tcctgggggt gttaagcgtt taggcagcag
      961 cgccctttca gaaaatattg gatcaatgat gaaataaatt gaatcaattc taatcaggaa
     1021 ggtattgtga ccaacccaca tgataaaatc agaatttcca agtgaatgta tcctctctaa
     1081 tgaatttgga ataacctgtg gcaaataact tttttcttct tcagtatatg aatatgagtt
     1141 attggaaaat ttccatttaa gaaaatggaa aaagcttcga tctttcatgg gcatccatgg
     1201 attaaaaaat tccccattct tataatgggg tgcatataaa aaagcagtat cagtatgtaa
     1261 tacatgatta tgccactgtt cttctgaaaa ttgtttgtct gatgtggaac aggaaaagaa
     1321 tactattagc agtataataa gtatttgttt cattgatgta ttggcatcag taaacaaaat
     1381 attaattata aaatgtcaaa agcctgttat ttttaataat attcttctca gagaatatct
     1441 ccagggcgtt tgctatttta tgcagcagtg attttttaca ttccaccttt tcgtgtttta
     1501 aatgccgtat aatcaactgt ctatcaataa attgcaaatc atagttaatt gtacatttag
     1561 ggtggttgta atgtatagaa tccacgccca gaagcctttg tatgttagca atctgttttt
     1621 cattcaacgg ttttgtgcag taaatggtat atgttctttt tgttgatttc atggccatct
     1681 ccataaacat aaaaattatt acttgaacta tcctgtatat aatatacaat agaaaaatca
     1741 aaaagttaca aatattttag aaaatactta aatatttttt aagtgagata gttatgagta
     1801 taattgatta ttttgaagca aatgatttca atgatgaaag ccttacaatg attaagggga
     1861 atagaaaaat ctatgtgata ttttccactt ttggagagcg tgaggcagag cttatatatc
     1921 aaaaaatacg tcttattgaa acattagcat gtattgatgg tatatttgtg tctcatcggc
     1981 gtgttgatga tgataaagaa gaattgaccg agaaaagagc taatgaagca agcagcaagg
     2041 caaaggttat agtatgcaat actattaatg tgcccgatat gggtcttgaa aaaggtaagg
     2101 gtgctgacat gcgaagagcc ctgtattatc ttattaccgg atataaggcg gatatacaca
     2161 atactgttgt agtttttctg gatgccgatg tccttgccca atactttggt acacattttg
     2221 tgttagggct ggctggcgca gtgttgcaag ggtatgattt tgccaaagca agtttctggc
     2281 ggcaaatggg cagggtaaag aagtatgtgg cacagccttt attttcggcg atagctcata
     2341 gaaatttaga tcccctgcga gaatttgcct atccactaag cggagaggtt gccggtacgc
     2401 tttcattttt tacaaaggtg agtttctggc agatgtatgg ggttgaaaca ggaataaata
     2461 tagatgcggt gttatccaac tttaaggttg cagatgttaa tttgggttta tatgatcatg
     2521 aacatcatgg cgatgtaaat atacagaaga tgtcatttgg aattatcaga acgtttttgc
     2581 ttcaattaat tgactatgga tacattgagt taaaaaatgg tgccgagata agcgatatat
     2641 tccaggctca gtatattgat gaacatggca ggcgacaatc actcgaattt aacctttccg
     2701 aaaaaaaata ttttccttta tgtacaataa tttaaaaaaa atttttttgt tgacatattt
     2761 tttgtatatt atatatgagt aagtactcac tcatatttta atgtgtttga gttattctta
     2821 cagtgatatg aaatttatag cactgttatt ctgagtgaag tgaagaaaca ttgtagtata
     2881 tggtggtgag tagaaaatgc attggccctg gcaagcgaag aaatttgaac agaacattgc
     2941 aagaaattgc gggttaacca agcaatctta ttttgaataa ataggtttgc tcatcccgtt
     3001 ttatctgaga ttgtcatagt gtttctgaat acattttaga ctaaaggggg gagaatatga
     3061 gttatgattt tatcaaatca cggttacatc tttatgccgt tttacaaaac attgaagatc
     3121 ttgttcagta tgatgaagaa gcaaaaaaac ttattaaaga ttggaacgtt gtaataaaat
     3181 tcagcgttaa ggggagtgga aatgtgtatc tgatttttga aaatggtaaa tgtaccgtta
     3241 cagatagctc tgaaaaaaaa ccagatattg tgttattttt tacttcacct gcgcatctga
     3301 ataaaatgtt tgatggcaaa gcaaatccta taccactgaa gggcataaca aaacttggct
     3361 ttttaacaaa agaatttcca aaactaactg aacgcctgga atattatttg aagcctgatg
     3421 accagaaatt gaaggatagc aggtttgtta caataaatac tgtaatgacg ttaaatactg
     3481 ctctatttgc actaaaagaa ttgcttcttt ttgatcccat tggaaaacac attgcaaaga
     3541 aaataaaagg aagcatggta atgatgaatg ttgataaggg gccggctgtg tatattgaac
     3601 aaaaaaacaa cgggtttgaa gtgcataagg gttatcatga acaaccactt gcagtgttac
     3661 aattcaaatc aatgaaaata gccaatgatt ttttaaacgg caagatggat gcattcagtg
     3721 caatagcatc tggtgctgtt gctactaaag ggcatattga atggttagat gcagtaagtc
     3781 ctgttctgga cagaattgct ttatatttag cataaatatt tttttatgag gtgttattat
     3841 gaaactagaa gctaaaggtg caccacaaag ttcagcagca aaaataaaaa gtaaaacgta
     3901 tacatattct aattctcata aactatttca gcgtgctttg aaagttatac catgtggtat
     3961 tcctggacat tttagcccgg ctccttttgt gccacctacc atgtatccat tttacgctga
     4021 tagggcaaag ggttcacggt tctgggatgt agatggtaat gagttcattg attacatgtg
     4081 tgcttatggt ccaatggtgt tgggatataa taatcccaaa gttgaatctg cagcaagcca
     4141 gcaacggcaa aagggtgatt gtatgacagc tccgggaaag gtgatggtgg aactggctga
     4201 aatgttaacc actaccattg ctggtgccga ctgggctttc tttgccaaaa atggtggtga
     4261 tactacaaat ttagctatta tgattgcccg tgctgcaact ggtcgaaaga aaattgttat
     4321 gataaaaggc ggatatcatg gtgttgctcc atggatgcag ggatatggcc actatggtgt
     4381 tatagaagaa gatattgcaa acataattgg tatagaatgg aataatgttc aacagtttga
     4441 agaggcaatt gctgctaatc cggatggtat tgcgggattt atagctacgc cttaccatca
     4501 tccaacattt gccgatagtg agttccctgc agaaggttac tggcagcgta ttcgtgagtt
     4561 gtgtaccaaa catggcatag tgcttattat tgatgatgtc cgttgtgggt tcaggcttga
     4621 tatgcgcgga tcaggtgcat attttgggtt taaacctgat ttagcctgtt actgtaaagc
     4681 tattgccaat gggtatccca tttcagcact agttggtatt gatgcactac gaactgttgc
     4741 tgcaaaagtt ttttatacag gaagttactg gtatcaggct gtacctatgg ctgcagcact
     4801 ggctacattg aaagaattaa aaaggattaa tggtcctgtt cttatgaaca agattggcaa
     4861 aaaacttttt gatggtatgg ttaaaatttg tggacagtat gggtatgatt taaaagttac
     4921 cggtgcatat tctatgccgt atgttcgcat taccaatgat ccaagcaata tgctgcatca
     4981 ggaatggtgt gctgagtgta cagctcgtgg tgcttatttt acttcgcatc ataactggtt
     5041 tgtatcagca gctcatacag agaaggatat tcagaagaca tggaatatcg ttgatgatgt
     5101 gtgcaaattg ttgaagaaga aatatggtga aaaatattaa tgcttgcaat tgtaagatac
     5161 aaggggttat catagcagta actatcgccc ataagaatta aataaaaaat tacaaaggac
     5221 aaggaggata tatatgccgt taagccagca ggaattaaaa caactgaacg aaaaagcatg
     5281 ccagataaga aaggatacca tagatactgt atactgggct ggtggtggac atgtgggtgg
     5341 ctcattaagt tttactgaaa tcatggtcat tctttattat aagtatttaa atattgatcc
     5401 caaaaatcca caatggcccg atagggaccg ggtggtactg agcaaagggc atgctggtgt
     5461 tggatatgct ccaatccttg ccgataaggg atatttccct aaagagttgt tgaaagagta
     5521 caaccacacc ggttcaatac tggggatgca cctggatggt aacaaggtac cgggtgttga
     5581 tgtgtcaact ggttcgctgg gacatggttt gcccatagcc gtagggttgg cattaggtgc
     5641 acgcttgcag aataaatcat ggctaacgta ttgtataatg ggagacggtg agtgtgatga
     5701 gggctcagtg tgggaagctg caatggctgc cgcccactat aaagttacta atttggtaac
     5761 tatcgtagac cgtaacagga tgatgataga tggtccaacg gaagaggtta tgcgccttga
     5821 accatttgct gataaatgga aagcttttgg ctggatagtg aaagaagttg atggacatga
     5881 ttttaatcaa ttggctgatg caattgaata tgcacaaaaa gaagataaag caccggttgt
     5941 tataattgca catacgatta agggcaagtg tgtggacttt atggaagata aatatgaatg
     6001 gcactatggc gggctggaca gtgaaaaggt agaacttgcc aaagcttgta tagacaaaca
     6061 ttattgtgaa atgtaaataa ggaggaaaac aatggctgct gatggattaa catatactat
     6121 cgttgacact gccaatatgt caaccgctga aatttatggt aaggtattat gtgatctggc
     6181 tgaaaaacat aatgatattg tgggactttc agctgattta gctaagtcaa caaagattgg
     6241 tcagtttgcg gcaaaatttc cagacaggtt ttttaatgca ggaattgctg aacagaatat
     6301 gtttggtatt gctgcagggc ttgcaaaatc ggggctcact ccgtttgttt ccacatttgc
     6361 ggtatttgca tccctgcgtg cggcggagca ggtgcgtact gatatttgtt atccaaagct
     6421 taatgtaaaa attattgcaa ctcatgccgg gacttcattt ggacaggcag gtaccacgca
     6481 tcactgtacc gaggatctgg ctgttatgcg ttcatttgcc aatatggttg tgatagctcc
     6541 tgcagatggt attgaaactg caaatgctgt gatagcagcg tatgagtaca aaggcccggt
     6601 atatatcagg attaaccgtg gttttgaccc tttcttatat gaaaaatctg attacggatt
     6661 tcagataggc aaagcagtac agcttaaaga tggcactgat atcaccatta tagcctgcgg
     6721 tgctgctgcg tataatgctt accatgctgc tggtatactt gaaaaagaag atggactgag
     6781 tgtgcgggta ctcaacatgc atacaattaa acccattgat gaagaggcca tccttaaagc
     6841 tgttatggaa acgcgccgca tagtgacatt tgaagatcat aacatttatg gtggacttgg
     6901 tagtgcagtt gctgaggtaa tagtaaaaag cggaaaagcc tgtgcctttg aatcagttgg
     6961 tattcccgat gaatatgcca tcatagggtt acctgaggat ttacagaatt actacggatt
     7021 tgatcagaat aagattgttg aaaaagtacg gcagataatg ggcaaagatt ttgaacaaac
     7081 tgaagactgg gaagacgagg tgtaatatgg caacccagaa cgaattgttt gcatcaacaa
     7141 tttttttaaa agctgcattg ccgcttacta aagtaatact ggaatacagg ccaaaaatgg
     7201 ctgcaaaata taaagggctc aacggtgtgg tgcagtttat tgcaaaggat gaggctggcg
     7261 atattggtgc atatctgcag tttactgatg gtgtactgga tgtaaagtat ggcattcatg
     7321 aaaaaccgga tatagtcttc acgtttaagg atgtaaagac tatgaatgca ttttttgccg
     7381 gtaagccagc ggtgccatct ataaaaggtt taactaaatt taaattgtta atgaaagcag
     7441 ttaatttatt actggctctt aagatcattg gcaggaaaac tcccccaaaa aaggcagaag
     7501 accgtgagct tatggtaagg cttatgctct atttgttacc acttggaata agccagctta
     7561 ataaagcagg tcatcctgaa atatcaaaat tttgtaaaaa aagtcccgac agaacgtttg
     7621 catggtcggt gaatggtact gatatagctt cgtatctgcg gatgaaggca gggaactcaa
     7681 aggcagggag gggcaaatat actcgttcaa agccatttgt agaatttaag tttgacagtg
     7741 tagatggtgc tttaggcatt ttattacaga caactgatat gattgaagcc tcaataaata
     7801 gaattcttgt tattgaaggt tcaccggaat atggcaaaga gataggttca ttcatgaata
     7861 cggttggaca attggcgtca taacattgtg aaaattttat gtatgttcct gttgtagaga
     7921 tgcctctaaa aggcatctct acaattggat tattgtgaat aaatagtaca tatacatcaa
     7981 tggaggtatc atgaaagtaa aatcattaag tcatgttgga ataaccgtca ccaattttga
     8041 aaaagcggta aaatggtact gggaaatgtt tagattccca cttgttgctg aagaaactat
     8101 ggatgaaaaa caggttgagg cattatacag tttgtatgga ttaaaaaata caaaattacg
     8161 acttgggttt ttacgtacac cgggtggtgc tgtgctggaa atatttgaat ttacacctgc
     8221 tatcccttcc gaaaaagtat gctggaataa gataggttta acacatatta ccctggatgt
     8281 acggaatgta aagaaatggt ataaaaagct aaaagacaag ggtgtaacgt ttttatgtga
     8341 acctcaaaaa acgggcccca atgaatgggt ttttatgcaa gatatggatg gcaaccttat
     8401 tgagcttatt gatttaaaat ttaactacat tgctctgaga tatttaggtg ccatagttgc
     8461 tcctctctta cgaagattac aatttgggaa gtattataag taaaagaaat tatagataga
     8521 ttaccaaata aataatactt cattaaataa aaatatttaa ttgtaaaaat tctaattatt
     8581 agtataccat agatgcattg taattgtatt ttaaaattct tattctactg ttttaataaa
     8641 aaattcttga atttatacaa aatttattat ctataatatg cattatacat tcattaacta
     8701 atagtaataa aaatataatt ataaacaata tttggaggat aaactatggg tgataaagaa
     8761 acgtataccg aagtaactaa aacatcatgg cttcaacggt tgggtggttc cataaaaagt
     8821 gttggtgtgg gttttcttct tttcattgca gcttttcctg tattatggaa aaatgaaggg
     8881 tgtgcagtta aggtggcaca gggacttaca cagggggctg ctgaagtaat ttcattgcca
     8941 gaacccaaag ttgatcttgc taataatggg aaacttatac acttttatgg tgatgccata
     9001 actgatgagg tgctgacaga ccctcttctt ggtataagtg aaaaagcaat acgtatagca
     9061 agaagtgttc agatgtacca gtggaaagaa ataaccaaaa gcaaaactga aaagaagtta
     9121 ggtggttcaa ccgaaacaac tactgagtat acctatgaaa aggtatggtc tgataacgtt
     9181 ataaattctg actcattcaa atacacccag ggacattcta atcctaaagc gatggcaatt
     9241 ccatctcaaa cgtttcaggc taaaaatgtt aaagttggcg attttgtttt aaatgatgaa
     9301 cttataagcc agattcaggg tggcgaagca attgcagttg atgcatcaaa agtagctaaa
     9361 gaatatgctg gtttggcaaa acaacttggc aatgaaatat acattggtaa tgatcccaat
     9421 aatccacaaa taggtgattt aagaattagt tgccaggttg ttcgtgcacc acaaaaagtt
     9481 tcagttattg cgcaacagca gggtaattat ctttcaccat tcctcactaa aacaaaaaca
     9541 acaatacaga tgttgcatac tggcattgtg agtgcagacc ttatgtttca ggaagcacag
     9601 gaaagtaata caataaggac gtggctggta agattgttgg gctttgggct tatgtttttt
     9661 ggcctaatga tgatattaaa gccactggta acattgggtg atgtagtccc atttattggc
     9721 agcattttac attttggtac aggagtagtg tctttcatta ttgctttttc tctttcgctt
     9781 attgttattg caattgcatg gattgcagcc aggccattac tggctattat attgttaatc
     9841 ataggcattg gggcctttat tacacttaag gtattaagta ctaaaaagac agcttcggca
     9901 tagaacatac taatacagaa tgcaattata aaggcaaggg ttagctcttg cctttataat
     9961 ttaatttttt attgattatt ttatctaaaa gtgtataatg atataaataa acaatatcta
    10021 tatacatgtc atatttaata tcgtcataca aatagagagg tgaatactat ggaaaaggct
    10081 actgacagtg gtgttacata ttttattggt attgatgcag gttccaccac actttctgtt
    10141 gttttaatgg ataaaaataa aaatattatt gatacatact atgcctatca tcatggaaaa
    10201 attaaagaaa ccattgaaca gtatttacag aaaaaacaaa taacaaaagt ttgtggtata
    10261 gctataacat catgcacacc acaggttttg aatgcttcca ttgtcaattc acaggttgcc
    10321 tatgttcagg cagctaaaga atatatgcca catgttcgta cactggttat tgttggtggt
    10381 gaaaaatttg gtgttattga atttgatgag tatggcaatt actcgcagta taggggaaac
    10441 acaagctgtg ctgcaggtac gggtggcttt ttagatcagc aggcacggcg attacagctt
    10501 gaaggttctc atcagctttc agagttggcg ctgaaaaatt atggcgatat tccaaagata
    10561 gcttcgcgct gtgcggtatt tgcaaagact gaccttattc atgtccagca agaaggttac
    10621 agtatttatg aaatatgtga tggcttgtgc tctggtcttg cacgcaatat tgctgatacc
    10681 ttgtttgcca gtaaaaagcc tctggagccg attgcagtgg ttggtggtgt gtcattaaac
    10741 aaagcagtta ttaaacactt tgaatcaatt attaatacaa aagttttagt gccggaacta
    10801 tcgcacctgt atggtagttt gggtgctgca ctatgggctg tttccaattc tgttagcgaa
    10861 cagatagcaa ttgaatttac acatgcaaat gaacaaaaag aataccacta caacaacctt
    10921 gatattgtat tgagccagta tcctgaattt gctggcattg aatcatatat gtttacatcc
    10981 aggcatgatg ttgaagttga tatctacacg ccatttatgc cacacactga agcctttatt
    11041 ggtattgata ttggttcaac aagtacaaag atggcagtga ccgatagttc cggtaatatg
    11101 cttgctggct tttatacacg tactgccggg aaaccgcttg aagcagtaca atcgctattt
    11161 gaagcacttg atagctatgt gcagcgtaaa ggtgttacat taaccattaa agaagcagcc
    11221 actacaggtt cagggcgaaa gttcatagga acacttattg gtgctgatgt gatccttgat
    11281 gaaatttccg cacatgccaa agctgcggta gcattgaatc cacaggtaga taccatcata
    11341 gagataggtg ggcaggattc aaaatttacc atgctgaaaa atggtaatgt ggtcttttcg
    11401 cagatgaaca atgtgtgtgc tgctggaaca ggtagcttta ttgaagagca ggcatataag
    11461 ctggggtgcc tactttcgga atatgctgac agggcgttgg gtaagcgctc accggttgca
    11521 agtgaccgat gcaccgtatt catggaacgc gatatcaact acctgatgag taaagggtat
    11581 tcaactgagg aattgcttgc cacagtgctt cattcggtag tggaaaatta tctttcaaag
    11641 gtttcaggtg aggcaaagat tggcaattgt atatgctttc agggagctac agcaaaaaat
    11701 agagcactgg tagcagcgtt tgaacagcgg ttgcaaaaac caatatttgt ttcaaagtac
    11761 tgccatttaa ctggcgcact gggttgtgca cttgaagtaa tggaaacata tagcggaaaa
    11821 acaaaattcc gtggtttaga cctgtataaa aaagatatac ctataaagaa tgaagtgtgt
    11881 gaactatgca ccaatcactg caagcttaaa gtggcaacta ttgataatga gattgttgca
    11941 tttggttttt tgtgtggcag ggattatcat tataaaaagt ttgtcagcaa aactaagggt
    12001 atatttgatt ttacaaaaga aagaaaaaag attctctcta cgtcaaccaa agataaaaca
    12061 gcaacagttg ggataccagc agcactacat ttagtagatg agcttgatct gtggattgat
    12121 ttcttttcac gtctgggtgt cagtgttacc acaagcatgg attgtcctga tccggtagct
    12181 actggtaagc gtttagcaca ggcagaattc tgtgcacctg tagcagcttt tcatggccat
    12241 attgcctatc tggttgataa atgcgattat atctttgcgc ccttctattt agaaaaggat
    12301 gcaccacagg aggcgcgcag gcaatactgt tattatacac aattcgcgcc tgcattggga
    12361 catcttctac ctgatgcttt tataaagtta ctatcgcccg tacttaatta tgaaatacaa
    12421 ccactgcaga gtagaatggc attatttaat gcatgtaaaa agatgaacat taatgctaat
    12481 taccttgaaa tcatgatggc atttgagcag gcactggcag cgcataaaga aaaaaacaaa
    12541 aagcttctga aagttggtac tcaatttttg aagtcacaca atggtatggc agtggtgttg
    12601 cttgggaggc catataatgc attgcttgat tcaatgaata agtccatacc ttcaatgttt
    12661 gtcaatttag gtataccagt gctgtatcag gatatgattg actatagcag taaggatgta
    12721 gaagacatta acgatttact gacagcattt cactggcatt atgcttcaaa gataattgaa
    12781 acagcacatg ttatttccag aaaagatgga ttatatcctg tgctggtaac atcgtttaag
    12841 tgtgcacctg attcgtttgt gatggaatac tttaaacgca ttatggataa agcaggtaag
    12901 ccgtatttaa ttttgcaact tgatgagcat gactcaaccg ttgggtatga aacacgtata
    12961 gaggcagcaa tacgttcttt taaaaatcac tatgcatcta agggtgtgcc tgcaaaacag
    13021 gttcgtgttg ttcctgcaat tacacgtgaa tccctacagg gaaagacagt gttgctaccc
    13081 aactgggata tcatggcagc gcgcctgctt agagcaaacc tgcagcgtga aggcattgat
    13141 gcccatatac tgtatgaaga tgatacccta attgcaaagg gtacgcgaag caataccggg
    13201 caatgtattc cgttaaacat tattgcgtat gaatacattg attatgttga aaagcataat
    13261 cttaatccag ctaaaacaat attgtggatg gctgattcag aaatatcctg taatatacga
    13321 atgtatccgt attttataaa aagtatattt gatagttacg gtaaaggatt tgaagaaaca
    13381 caggtttttg ttggcgatat atcattcagg gaagtttcag taaaggcagc aatctatacc
    13441 tattttgcct ttatgttcag tgggttgctg cgcagagcag gttgccattt caggccgtat
    13501 gaatgcattc caggttccac tgataaagcc gttgagcaca ccatgcagga atttgagcaa
    13561 gctttcaggg atgaaataga atggtcagat gcactgggta atatggttca gcgattcaaa
    13621 acaataaaag ttaagcgtac aaaccgtccc aaagttgcaa tatttggcga cctgtatgtt
    13681 cgtgacaacg atgttatgaa tcaaaacctg atagctttta ttgaaaaaca tggtggtgag
    13741 gttatcacaa caccatacaa ttattatgcc aagatgatag caaatccata ttttaaaaaa
    13801 tggttcagag agggtaaata caaggcgata gttaccggaa agacattgct gacagcagtt
    13861 gaacttatgg aaaagaaata ctacagttat tttgaaacat tgctaaatga gccaaatccg
    13921 gtatatactg ttgactttga aaagctattg tcacagtttg gcgttacgat gtatcatacc
    13981 ggtgaatcat ttgataatct tatcaagata ttttcacttt tacaacatta tcctgatatt
    14041 aaactctttg tacaaacaag tccagcattc tgctgtccgt ctcttattac cgaagctatg
    14101 gcgcataaga tttatgaggt tacaggagtg ccagtggtaa gcttaaccta tgatgggact
    14161 ggtagcttta aaaatgatgc agtagtgcca tatttaacgt tattacggga tactgttgtc
    14221 cagaaagaag cagccaaaaa gctatcgtag atgtgttaat ggagaacatg gttcaattat
    14281 gccttaactg aaacaaaact cacgcacatt gcagtaatgg tatgtaacgc aacaagtgta
    14341 tatccagtgg gtaagtccag cgtataagac aagcatatag caatgatgtt aataattgtt
    14401 cctgcaatcc aggcaattat aagtggatgc tttacatgta gtgacagtgc aatcattgct
    14461 ggtgaaagca gcagggcaaa cactataagt acgcccgcca gtttaactga acttgtaaca
    14521 gtgatagcaa agataatgaa aatgcataca aaaggccaag ctacaaaaaa tattcttatt
    14581 ttggtagcac acccctttgc tttgccattt ccatcatctg ttcaccactg gtttttggtg
    14641 gaactttttt agattgttct ttttgtacaa gctttacggc atcattggta catgccatta
    14701 cacacagacc acatccaata cagcggtcta cgttaacatg ggctatgtcg gtaatctcaa
    14761 ttgcatccat ctggcacata tcaacacagt tgccacacga tgtgcagtta tcattggttt
    14821 gcgcataata atttgaaaat accatctctg caggttttgg aaatcgcttt attgataaaa
    14881 gcactgcgca gcagtcacca cagcaactac acataccagc agggttttgt gcagtgccgg
    14941 gttgagttac catgccgtgt ttttgtgctt cagtaactat cgctatagct tcatcagcac
    15001 ttatttttct tcctaattta ttttctatat aatactcagc catggaacca aacatgagac
    15061 aggtttccat tggcttatca cagcctttgc caataatctt tttttcctta cgacatatac
    15121 aatcagcaac tgctattaca gaggcttgct taatgattgc tactgcatct tcaaagggtg
    15181 caacagtgct ttttgtttca attgattcat ttactggaat agggcgtaaa aacatattgg
    15241 cacttttagc cacagctttt gataatcctt cctcataata ttgctccagt agcatggcaa
    15301 ggtctttgct taagcgctta acctgaaatt caaacacacc gtgtataaac ggtatggcgc
    15361 catattttgc aaccccgtct tttgtctttt taaataaaag gccccgtttt accatatctt
    15421 ctaaatgttt cgttagttct tcctgttgtc ttcctgtacg tttggcaata ctttcagcag
    15481 tttcaagcat gggcgatagt tctaagaaaa gctttgcatc ctcttcggaa aatagtttct
    15541 ttaatattgt tatttctatc cttgactgtg tttcaggaaa tcccagtgag tacatgtcca
    15601 ggcgtttctg caatgctctg tatatatctt ccataggctt atcctcctat attgatttat
    15661 taaactggaa taaactagca caagaacaag caaaataaca ccgttatgtt cgtcaaatta
    15721 atttttt
//
LOCUS       DAORYL010000072        15277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_3123_length_15277_cov_14.181579, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000072 DAORYL010000000
VERSION     DAORYL010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15277
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3123_length_15277_cov_14.181579"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..516
                     /locus_tag="PLV81_11835"
     CDS             193..516
                     /locus_tag="PLV81_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOM10831.1"
                     /translation="MKENLNNIENRIEEKINKITHLIVEKTDPEKIILFGSRAKGTAH
                     KGSDIDIALEGVQKLSVRDYRLLKEEIDNISGLYSVDIVFIEKVDNSFKQIIQDTGVI
                     LYEKK"
     gene            503..895
                     /locus_tag="PLV81_11840"
     CDS             503..895
                     /locus_tag="PLV81_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HOM10832.1"
                     /translation="MKKSDIYIAIEKFEKAIASLREGVNQTNTDLERDGTIQRFEYTV
                     ELLWKTLKIILTYYGVECNNPRTCIKEAFRNNLIDDDEILLDMLEDRNLSSHIYDEDT
                     SKEIFERIKNIYINILERVVQMVKSNIK"
     gene            957..2270
                     /locus_tag="PLV81_11845"
     CDS             957..2270
                     /locus_tag="PLV81_11845"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOM10833.1"
                     /translation="MSLVKLVSDVIVIGSGPGGATVARELSHAGKKVIILDKGADHRG
                     RIYYGTYPGAMIYSDKMSLLFTKEGLNIISPVMVGGATSMYCGCAAPPPVWLKKRYRI
                     DIDKEVNEAIQELNIQPLPPQLRGIASTRIAQAASQLGYDWFPQPKFMNPERSKHFHC
                     TAACMLGCRCGAKWNAAEWVDEALQSGAALYTHVKIKKILHDGRSVQGVEGTIDGKKF
                     IAHAPYVVVAAGGIGSPRLLQASGFKDAGKGMTMDVTVMVYGTISDKGIGKEPPMTWS
                     WENDADGYMLSTLIDPWLLYPLAAMRVGVKPLLQWFQWNHILGVMIKLKDDIAGGVYS
                     DGTISKPLTHKDKERLAKARAVCEAILKKAGAPASSIFMRPLIGTHPSGTVRIGDMLD
                     TNLQTKIDGLYVCDASTFPESLDRPTVLTIIGLGKRLAKHLIKKR"
     gene            2280..3629
                     /locus_tag="PLV81_11850"
     CDS             2280..3629
                     /locus_tag="PLV81_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOM10834.1"
                     /translation="MNGLKRVLGLPAVTFIAVGFTIGGGVFVFTGIVLKMVGPALPLA
                     YLLAVVPVFLSMLPLAMLGSAIPSVGGNYKYPSRMVSPGIAFVGIWIYVLATFFGQIP
                     LYSISCARYISTFYPELSLKICAIALVTLFYIVNLIGIKPAATVQAIMVIVLLSALLF
                     FSFSGIARFDVANFANFFEKGAGNLMVGVALLTFTYLGSNGIIELGGEIQNPGTTIPR
                     AYFITFPVVTAVYFCVALATAGSMPWQKTAQEAEPLIFLSRSFLSNAGVMFFIIGGAV
                     LALTTTLNALFIVGTKSLLIIIQDKLLPSWLGSIHQTFGTPHILLSVIWLLSIVGIVS
                     DLSIETFASYSALGGIIIFLPILIAALKFPRLYPEKYQKAAFKLTGAWLHVCVIVGFV
                     LILFFCLVILIDLKTMFKIGLFIVFVLSGVIYYFLRKRYLAKQGIHLEAITQKEDWG"
     gene            3633..4703
                     /locus_tag="PLV81_11855"
     CDS             3633..4703
                     /locus_tag="PLV81_11855"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOM10835.1"
                     /translation="MSELLLYKVFPDLQKLPHVSLGNFPTPVQRLTSLGHDSLWIKRD
                     DVSSALYGGNKVRKLEFTLAEAIVTGKKKVVTMGGIGTNHGLATAIFCKHLGLGCRLL
                     LFWQPVTEYVKRNLLLFVRYGAEIHYYKTMLKTGFMFFTKERLAHDAYLLYAGGSSPL
                     GTVGFVNAAFELKSQIEKNELPEPDYIVCALGSAGTMAGLWLGAKLAGLKSTVVGVRV
                     TDRSLGPVPIANEKSVLSLITKTYTLMKSVTPLPPINVTTPVILHDWCGEGYGYPTGA
                     CLDTIETMKTSEHIQLEPTYTGKTFAAVCDMIQKKEYADKTILYWHTYNSVDLSQEVK
                     KANYHDLPQSLHWVFEDYNSLQ"
     gene            4724..5059
                     /locus_tag="PLV81_11860"
     CDS             4724..5059
                     /locus_tag="PLV81_11860"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOM10836.1"
                     /translation="MRYFFIMEHGTTIIERLNQFFKSRNNVEKVLLFGSYARNTQTKR
                     SDIDIIIITETNKRFFDRYDDFNDIYNIVNNHCDLLIYTPSEWENIRQRKFFKNILKE
                     AKEIYVKGR"
     gene            5043..5465
                     /locus_tag="PLV81_11865"
     CDS             5043..5465
                     /locus_tag="PLV81_11865"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOM10837.1"
                     /translation="MSKEDNIQESLRWFDTAMSDYEAAKIMCENKKYAHACFLSQQSA
                     EKALKALHYYYDSDPWGHSVLKLIDELKYIDIEVYTILALYTDNARLLDKLYIPTRYP
                     NGLPDITPDLAYSKKDADEALLSADMILQKVKEIIAKR"
     gene            5529..6008
                     /locus_tag="PLV81_11870"
     CDS             5529..6008
                     /locus_tag="PLV81_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOM10838.1"
                     /translation="MASTQCEVPAFNPEDSEIKEILSQYKIIAVVGLSDDPSKDSNRV
                     AQYLIHHGYTVVPVNPTKETILGLKCYPNLKSIPFPVDIVDIFRKPADVPPVVDDAIA
                     IGAKVVWMQLGIAHNEAAKKAKEAGLKVVQSKCIKMEHTRYFGTGQETVSFNINLNQ"
     gene            complement(5992..7380)
                     /locus_tag="PLV81_11875"
     CDS             complement(5992..7380)
                     /locus_tag="PLV81_11875"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOM10839.1"
                     /translation="MKAKIQKLKKELHKALSLSDDPDILEKYAVDETSDLKGNPWLVV
                     YAKNTQDVSLVLSLCDTYRIPVIPRGAGTGVTGGAVPVSGGVVLSLEKMNKILQIDTE
                     NMIAVCQPGVITGQLQKEALQHGLMYPPDPASLDSCSLGGNVAEGAGGPRAVKYGTTK
                     DYITGVEFVLADGSIIQHGGKFIKNATGYNLTGILIGSEGTLAVITTIYCKLIPVPAH
                     TLDMLIPFQSLHDAVQMVYTLLINRIRPAVLEFMEEDALTLAAQYLGQDMPHPEARAH
                     LLIQLDGDSYHEIDESLQKILSLGAVDPNSILVAQSPEQKERLWKARRCIREAIHAKS
                     PVFLAEDCSVPRSKIVPFLTGVKQALTPYGLQSIMFGHAGDGNVHIDVLKNDMPYDEF
                     KALVPSLKEIIYAEAYKYGGTITGEHGTGFIRKDALKKFASSQELSLYKRLKKAFDPN
                     GILNPHKIIDLD"
     gene            complement(7588..8778)
                     /locus_tag="PLV81_11880"
     CDS             complement(7588..8778)
                     /locus_tag="PLV81_11880"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOM10840.1"
                     /translation="MEQQKKLYTDTNLLIAFGITLVVVMGVTNITPALPAMAQYFAIP
                     YSSVTLVITVFTMPGIVLTPLLGIVADRIGRKIIIIPSLILFGITGIIMFFITDFTWL
                     IILRFIQGMGVSALGAINATIIGDMFSGKDRTIAFGYNASVLNIGTAAYPAIGGFLCI
                     LGWNYPFLLSALALVVAWFAIFHLHNPEPVTTLSLKRYFHAIANIIKSPYVIGLLSTT
                     VLTFILLYGPIITYFPYIISYRFKGTAATIGLFMSLMSIVTAITSSQLQILTKRYAEK
                     NLILYGFIGLCISFVIAGFAYNYFWLAIATIIGGVSNAINNPSLLSLLTAAAPAQYRG
                     AIMSLNGMGLRIGQTVGPIIMAQLCAIVTVDKAFYTISGLTLVIVIALFSTLLSNRYC
                     TLKR"
     gene            9074..10888
                     /locus_tag="PLV81_11885"
     CDS             9074..10888
                     /locus_tag="PLV81_11885"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOM10841.1"
                     /translation="MAQFKSESMSAVFQEQAAKLGSKACVSYKKGSQWVDISWADMNT
                     MVHNMAYYLMDFGIKKGDRVAIFSPNRWEWWVASLATTSIGAVSVPIYATNSAEEAQY
                     VLEHSGSSICFVGTEDHADRVLKVIKKCPKLDQLVIFDDLAKKKQNVITFKEALEKGA
                     KKAKPAEFEKRLKAIKRDDVSTIIYTSGTTGNPKGVMLSHYNFYSNAKNIMADMGKFF
                     NTEDVWLSFLPLSHSLEMTCGFYGPIWVGGKTAFAESIEKLLDNFKEVRPTVIISVPR
                     IYEKVHAGILAQVAAAPGLKKAIFNFALNTAKKNLPYVCRDLPRKGLFAIRYNIADKL
                     VFSKLKAAIGMDRLRYAISGGAPLSVSDAEFFIGMGMKILEGFGLTETTPVLTYNRPW
                     FIKPGTVGQAIPETKIKIADDGEILAKGPQIMLGYYKNKTATEEVMTKDGYFKTGDIG
                     VIDEDGFLKITGRIKDIIVTAGGKNISPQNIENSVKTSPYVEQIAVIGDKRKYLSALV
                     IPNFEALGKWAKQKGISFSSNADLIKNDEVNKLIESEIAKYTKQFSRVEQIKKFTLLE
                     AEWTQATGELTPTQKVKRRIIEQKYAKEIEAMYPPDIE"
     gene            11052..12512
                     /locus_tag="PLV81_11890"
     CDS             11052..12512
                     /locus_tag="PLV81_11890"
                     /inference="COORDINATES: protein motif:HMM:NF006113.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HOM10842.1"
                     /translation="MIKNVLKIVGILILIFIIILLVKAITISPKQVEVKEAIPYKIDS
                     MIVAQHLSDAIQIQTVSNQDQAKTDVKQFEQFHKFLAKTYPALHKKLKKEIVGGYALL
                     YTWKGSDPSLKPMLLMAHQDVVPVEELTKDQWKYPGFSGTIADGFIWGRGALDIKNQL
                     ISIMEAVEFLINKGFVPKRTIYLAFGHDEEVGGEGAKAIAKLLKQRNVQLEYVLDEGG
                     AIMEGMLPGVETPIALVGIAEKGYLTVKLTVKGEGGHSSMPPHHTALGILSKALVDVE
                     DNPFPKNFDSPARQMFEYVAPHMKFPLRLLFANMWCFKPLLKWQFEKSESTNAMIRTT
                     AAVTMAGASEKENVLPTQAWAMINCRILPGETMDSTIAYLKKVIGNDAVTIEATPWSN
                     DPSPVSDIQAGSFTSIATIARQINPDVIVAPYLVLGATDSRHYNALTNQIYRFSPVKM
                     NSEDLKRVHGINERISISDMGKSVKFFIALIEKTHM"
     gene            12514..13164
                     /locus_tag="PLV81_11895"
     CDS             12514..13164
                     /locus_tag="PLV81_11895"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOM10843.1"
                     /translation="MCGRFAQIEPVDVIAQRLQVEEVLVQHTAPRYNISPGEDIVALV
                     QTPGKTLIEIRWGLIPYWAKEPSSFKPLINARVETVIQKPSFKRAFFNNRCIIPASGF
                     YEWKKEGAKKIPYFITVEDFFGFAGIFDSVTVNDKQLLTCAILTTAASEQMSIIHNRM
                     PVIIRQSEFDQWINKETSPEILTKLMQPFNDRLVIYPVSTKVNNPSYKEPDCIHPV"
     gene            complement(13254..>15277)
                     /locus_tag="PLV81_11900"
     CDS             complement(13254..>15277)
                     /locus_tag="PLV81_11900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM10844.1"
                     /translation="SLILLRRGKFQIAANISFASVQIALWIVMFFEKGQILQRLDSIT
                     LVAASLVYTPLLFIERRRGIWFYYTANMLLLIIFTLYSRFHLGLQRWEAIEYFMDSSL
                     ALTFVAVISYLIFSIHKLGIINAKKAEEKIQRQYEELSATNEELEAMNEELAAINEEL
                     EQTQQELLVSNINLQKETARLQAILVSIADGIVVTDENGIITSINAAALKLFGLHNDV
                     IGEQFKEYCNIYNAKTGTPYADPVTQVLTSGKPVSLYRHTVIRNNGDIYHTHIVCTPL
                     YVANRIDGCIVDIRDMTDMVRYEEELIKTAKYESLSIFAGGIAHDFNNMLSAILGNIS
                     IIEQLTPNNEKVLKASERAQKAIMKARDLTTQLLAFSKGGAPVKTSTSLVNIIKDTAE
                     FVLSGSPIKCNVEFENNLHNALVDPTQISQVIHNIILNARQAIKGQGTITITAKNATL
                     DDPGIPVKGNFIAISIHDTGEGIPPDIMPYIFDPFFTTRDGGHGLGLSIAYSIVKKHD
                     GHITVNSSKEGTLFTIYLPVAPPNETSVYETTESYCTPSKSHILIMDDDALIAESLNE
                     MLALQGCTAVTVSNGDDAINAFADAIKQNKPFSIAILDLTIKGGKGGVEIVTELQKFK
                     TSCKFIATTGYTNEPIIANYRDYGFDAILRKPFTMNELTQILSQLTKEN"
BASE COUNT         4757 a         2716 c         3251 g         4553 t
ORIGIN      
        1 tacaacgctt ctggtggaaa aaattgaaaa gaattagggt aaattttgta atttttaata
       61 aaaaaatttt acaacaaggg gtcagagcct tatatatttg atttgacaaa gtaaaacttt
      121 tgcatacaat aaaacattag aaataatgtt ctaaaacgat tttataaaaa gagaataaat
      181 taaatatatg gtatgaaaga aaatttgaac aatattgaaa accgtataga agaaaaaata
      241 aataagatta cccatctcat tgtagaaaag actgatcctg aaaaaataat actatttggt
      301 tccagagcaa aaggtacagc acataagggt tcagatattg atattgccct ggaaggtgta
      361 caaaaattat cagtacgtga ttatcgcttg ctaaaagaag aaattgataa cataagtgga
      421 ttatattcag ttgatatagt ttttattgaa aaggttgata atagttttaa acaaattatc
      481 caggatacgg gagttattct atatgaaaaa aagtgatatc tatattgcta tagaaaaatt
      541 tgaaaaagcc attgcatcat tgcgtgaagg agtgaaccag acaaatactg atctggaaag
      601 agatggtaca atccagcgtt ttgagtatac tgtagaattg ttgtggaaaa ctttgaaaat
      661 tatattaacc tattatggcg ttgagtgtaa taatccaaga acgtgcataa aagaagcatt
      721 tagaaataac cttatagatg atgatgagat actactggat atgcttgagg atagaaattt
      781 aagttcacat atttatgacg aagacacatc aaaggaaatt tttgaacgga ttaaaaacat
      841 ttatattaat atactcgaac gagtagttca aatggttaaa agtaatatta agtaatatgg
      901 tatttgtaat tacattaatc agaatagata cataatacat tataaggagg tttgccatgt
      961 cgctcgtgaa acttgtttca gatgttatag tgattggatc gggccctggt ggggcaacag
     1021 ttgctcggga actatcgcat gctgggaaaa aagtcattat attggataaa ggagctgatc
     1081 accgcgggcg catttactat gggacgtatc ctggtgcaat gatttattct gataagatga
     1141 gcctgctttt taccaaagaa gggcttaata tcataagccc agttatggtt ggcggtgcaa
     1201 ccagtatgta ctgtggttgc gcagcgccac cgcctgtatg gctcaaaaaa cgctatcgaa
     1261 ttgacattga taaagaagtc aatgaagcaa ttcaggaact gaatatacag cccctgccac
     1321 cacagctaag gggaatagct tcaacgcgga ttgctcaggc tgccagtcaa ctgggatatg
     1381 actggtttcc acagccaaaa tttatgaatc ctgaacgtag caaacatttc cattgtactg
     1441 cagcgtgtat gctgggatgc aggtgtggtg caaagtggaa cgctgccgaa tgggtggatg
     1501 aagcgttaca gtcaggagca gcattatata cacatgtaaa gataaaaaag atactccacg
     1561 atggaagatc agtgcaggga gttgaaggca ctattgacgg gaaaaaattt attgctcatg
     1621 ctccgtatgt agtagttgca gcaggaggta ttggaagccc acgcttgtta caggcttctg
     1681 gatttaagga tgcaggtaaa ggcatgacca tggatgttac tgttatggta tatggtacaa
     1741 tcagtgataa aggtattggc aaagaaccgc caatgacatg gtcatgggaa aatgatgcag
     1801 atggctacat gctctcaacg cttattgacc catggttgtt gtatccgctg gcggcaatgc
     1861 gtgtgggggt taaaccattg ttacagtggt ttcagtggaa ccatattctt ggggtaatga
     1921 ttaaacttaa agacgatata gccggaggag tttattctga cggaactatc tccaaaccat
     1981 taacgcacaa agacaaagaa cgcctggcaa aagcacgtgc ggtgtgtgaa gcaatactta
     2041 aaaaagcagg tgctcctgct tcatcaattt tcatgcgccc attaattggc actcacccca
     2101 gcgggaccgt gcgcattggc gatatgcttg ataccaatct tcagacaaag attgatggcc
     2161 tgtatgtttg tgatgcaagc acgttccctg aatcactgga ccgtcctaca gtacttacca
     2221 tcattggatt aggcaagcgc ctagcaaagc accttattaa aaaacggtag aggtgcctta
     2281 tgaatgggct aaaacgggta cttgggcttc cggcagtaac ctttattgct gttgggttta
     2341 ccattggggg tggcgttttt gtattcaccg gtatagtatt gaaaatggtg gggcctgcct
     2401 tgccgcttgc ataccttctg gctgttgttc ctgtattttt aagcatgctg ccactggcaa
     2461 tgttaggttc agccattcca tcggtgggtg gcaattataa gtatccaagc cgaatggtct
     2521 caccaggcat tgcatttgtg ggtatatgga tatatgtgct ggcaacattt tttgggcaaa
     2581 ttcccttata cagcatttcg tgtgcacgct acatcagcac cttttacccg gagctatcgc
     2641 tcaaaatatg tgcgatagct cttgtgacat tgttctatat tgtgaacctc attggcatta
     2701 agccggcagc aaccgtgcag gccatcatgg tgatagtgtt gctcagtgcg ttgctgtttt
     2761 tttcgttcag tggtattgca cggtttgatg ttgcaaactt tgccaatttt tttgaaaagg
     2821 gagcaggtaa ccttatggtg ggggtggcat tgttgacctt tacgtattta ggatcaaatg
     2881 gtatcattga attaggtggt gaaatacaga atcctggcac cacaattccc cgagcgtact
     2941 ttatcacatt tcctgttgtc accgcagtgt atttttgtgt ggctttggca actgctggta
     3001 gtatgccctg gcaaaaaaca gcacaggaag cagaaccttt gatcttttta agcaggtcgt
     3061 ttttaagcaa tgctggtgtc atgtttttta ttataggtgg cgcagtgctg gcacttacca
     3121 caacactcaa tgccctgttt atcgttggga caaaatcgct gttaataatt atacaggaca
     3181 aattgttacc atcctggctt ggaagcatcc atcaaacatt tggaacgcca cacatattac
     3241 ttagtgtgat atggctgttg tccattgtgg gaatagtttc tgatctttcc atagaaacat
     3301 ttgcgtcata ttcagcactg ggtggcataa ttatttttct gccaattctc atagcagcac
     3361 taaagtttcc gcgattgtat ccggaaaaat accagaaggc agcattcaag cttacaggtg
     3421 catggctgca tgtatgtgtt attgttggtt ttgtgctgat acttttcttt tgccttgtga
     3481 tacttattga ccttaaaacc atgttcaaga ttggattgtt tattgtattt gttttaagcg
     3541 gtgtcatcta ctattttttg cgaaagcgct atttagcaaa acaggggatt catcttgaag
     3601 caattacaca aaaggaagat tgggggtaag ctgtgagtga attgttatta tataaagtat
     3661 ttcctgactt acaaaaacta ccccacgtat cattgggaaa ttttcccaca cctgttcaaa
     3721 ggcttacatc attggggcac gatagtttat ggattaaacg cgatgatgta tcatcggcgc
     3781 tgtatggggg aaacaaggtg cggaaattgg aatttacatt agcggaggcg atagtaaccg
     3841 ggaaaaagaa agttgtgaca atgggaggca ttggtaccaa tcatgggctg gcaaccgcta
     3901 tcttctgcaa acaccttggg cttggatgca ggttgcttct attctggcaa cctgtgacag
     3961 aatatgtaaa acgaaatctg cttttatttg tacgctatgg tgcagagata cactattaca
     4021 aaacaatgct gaaaacaggc tttatgtttt tcacaaagga acggttagcg cacgatgcat
     4081 acttattata tgctggcggt tcttcgccat tggggacagt gggatttgtt aatgctgcct
     4141 ttgaactaaa aagccagata gaaaaaaatg agttgcctga gcctgattat attgtgtgtg
     4201 cattgggttc cgctggtacc atggctggct tgtggttggg tgcaaaacta gcgggtttaa
     4261 aaagcactgt ggtaggagtc agggtaaccg acagatcact ggggcctgtg ccaatagcca
     4321 atgaaaaaag tgtcctgtca ttgattacaa aaacatatac tttgatgaag agcgtcacac
     4381 ctttgccacc cattaatgtt acaacgcctg ttatattgca tgactggtgt ggtgaaggct
     4441 atggatatcc aaccggcgca tgcctggata ctatcgaaac aatgaaaacg agtgagcata
     4501 ttcaacttga accaacctat accggcaaaa cctttgcagc cgtttgtgat atgattcaga
     4561 aaaaagaata tgctgataaa accatactgt actggcatac ctacaactca gtagatttat
     4621 cacaggaagt taaaaaggcc aattaccacg atttaccaca aagcttacac tgggtgtttg
     4681 aagattataa tagcttacaa taagtattga catgatactg taaatgcgct acttttttat
     4741 catggaacat ggtactacca ttattgagcg tttgaatcag ttttttaaaa gtaggaacaa
     4801 tgttgaaaag gtgctgttgt ttggttccta tgctcgcaat acgcaaacaa agcgcagtga
     4861 tattgatatt atcataataa ctgaaaccaa taaacgtttt tttgatcgct atgatgattt
     4921 taatgatata tacaatattg taaataatca ctgcgactta cttatttata ctccttcaga
     4981 atgggaaaat attcgtcaaa gaaaattttt taaaaatata ctgaaagaag caaaagaaat
     5041 atatgtcaaa ggaagataat atacaagaat cactccgttg gtttgataca gcaatgagcg
     5101 attatgaagc tgctaaaata atgtgtgaaa acaaaaagta tgctcatgcg tgttttttga
     5161 gccagcaatc agctgaaaaa gcattgaaag cattacatta ctattatgat tcagatccat
     5221 ggggacattc ggttttaaaa ttaattgatg agttgaaata tatagatatt gaagtatata
     5281 ctatattagc attgtatact gataatgcac gtttattaga taaattgtat ataccaacac
     5341 gttacccaaa cggtttgcct gacattacac ctgatctagc atattcaaaa aaagatgcag
     5401 atgaagcatt attgagtgct gatatgattt tgcaaaaagt taaagaaatt atagcaaaaa
     5461 ggtaatttta tacttgccgg ttcaaaggta tcatagtatc ttaataacaa ttttttcagg
     5521 agttttttat ggccagtaca caatgtgaag ttccagcatt taatccagag gatagtgaaa
     5581 taaaagaaat cctttcacaa tacaagatta tagctgttgt tgggctatcc gatgatcctt
     5641 ctaaggatag taacagggtg gcgcagtatt tgatacatca tggatacacg gtggttccgg
     5701 ttaatccaac aaaagaaaca atactgggac ttaaatgtta tcctaattta aaatccatac
     5761 ctttccctgt ggacatagtg gacattttcc gcaagcctgc cgatgtaccg ccggtggtgg
     5821 atgatgctat cgccattggg gcaaaagttg tgtggatgca gttaggcatt gctcataacg
     5881 aagcagccaa aaaagctaaa gaagcaggtt taaaggtggt gcagagcaaa tgcataaaaa
     5941 tggagcatac ccgctatttt ggaacgggac aggaaacagt ctcatttaat attaatctaa
     6001 atcaatgatt ttgtgagggt ttaaaattcc attgggatca aacgcttttt ttaagcgctt
     6061 ataaagcgat agttcctgtg atgaggcaaa tttttttaac gcatctttac gtataaatcc
     6121 agttccatgc tctccagtga tagtcccgcc atatttatat gcttctgcgt aaataatctc
     6181 ttttaatgat ggtactaacg ccttgaattc atcatatggc atgtcatttt taagcacatc
     6241 gatgtgaaca ttgccatcac cagcatgacc aaacatgatg gattgaagcc cataaggggt
     6301 cagagcctgt tttacacctg tcaaaaatgg cactatcttt gagcggggta cagagcaatc
     6361 ttcggccaga aaaacaggac tttttgcatg tattgcctca cgtatacagc gccgtgcttt
     6421 ccacaggcgc tctttctgtt caggagattg tgctaccaat atgctgttgg gatcaactgc
     6481 gccaagtgaa aggatttttt gtaacgattc atcaatttca tggtaactat cgccatctaa
     6541 ctggatgagt aagtgggcac gagcctctgg atgtggcata tcctgcccca aatactgtgc
     6601 agcaagggtc agagcatctt cttccataaa ttcaagcacc gcaggccgta tgcgattgat
     6661 taaaagtgtg tagaccatct gcacggcatc atgaaggctt tgaaacggta tgagcatgtc
     6721 aagcgtatgt gcaggaactg gtatgagctt acagtaaatt gttgttataa cagcaagagt
     6781 gccctctgaa ccaatgagta tgccggtaag gttatagccg gtagcgtttt ttatgaattt
     6841 gccaccatgc tgtataatgc taccatcggc aagaacaaac tctacaccag taatgtagtc
     6901 ctttgtggtg ccgtatttta cagcacgtgg tccaccagca ccttcggcaa cattgccacc
     6961 aagggaacac gaatccagac tcgctggatc cggtggatac atcagaccat gctgtagtgc
     7021 ttccttttgt agttggccgg tgataacacc tggctgacag acagctatca tgttttcagt
     7081 atctatctga agtattttgt tcattttttc aagcgatagc accacaccac cagatactgg
     7141 cactgcacca ccggtaacgc cagtgccggc accgcgcggt ataaccggta tcctgtacgt
     7201 atcacagagt gaaagcacca ggctaacatc ctgagtattt tttgcataca ccacaagcca
     7261 cggattgcct ttaagatcag aggtttcgtc aacggcatat ttttctaaaa tatcggggtc
     7321 atcactcaat gacaatgcct tgtgcagttc ttttttcaac ttttgtattt ttgctttcat
     7381 atgttcagct ttctacctat agtactggtg tagatttctg tacgtcacat catagccatg
     7441 atatggaatg gcacgtaaaa ttttttacaa gaaaaaataa tatataaatg caactttttt
     7501 gaaaattaga ttaatcaaaa aatggttttg ttgagacaac gtcattagtg caccaatgct
     7561 attgccggtc aatttcattg gcagacatta cctcttcagt gtgcaatatc tatttgatag
     7621 caatgtggaa aaaagtgcaa tgacaattac aagtgtcagg ccacttatag tataaaatgc
     7681 tttatcaaca gtaactatcg cacataactg tgccataatt attggaccaa ctgtttgtcc
     7741 aatacgcaat cccatgccat tgagtgacat gatggcaccg cggtattgtg ctggtgctgc
     7801 tgcagttaaa agcgatagta aactgggatt gttgattgca ttggagactc caccaataat
     7861 agtggctatt gcaagccaga agtaattgta tgcaaaacct gctatgacaa acgatataca
     7921 aagccctata aagccataca gtatgagatt tttttctgca tagcgttttg taaggatttg
     7981 taattgcgat gatgtgatgg cagttactat ggacataaga gacatgaaaa gaccaatagt
     8041 tgctgctgtc cctttaaacc ggtatgatat gatataggga aaataggtta taattggccc
     8101 atacagaagt atgaatgtga gcacagttgt tgacaggagt ccaatgacat agggactttt
     8161 tatgatattg gctattgcat gaaaatagcg ttttaatgat aaagtagtaa ctggctcagg
     8221 attgtgtaaa tggaatatgg caaaccatgc cacaacaagt gctaaggctg atagtaaaaa
     8281 gggataattc cagcctagga tgcacaagaa tccccctata gcagggtacg cagcagtacc
     8341 aatatttaac acactggcgt tgtatccaaa tgcaatggtg cggtccttcc cactgaacat
     8401 atcaccaatg atggttgcat taatggctcc taatgctgaa acgcccatgc cctgaataaa
     8461 ccgtaatata ataagccagg taaagtcagt aataaaaaac atgataattc cagtaatacc
     8521 aaatagtatc agtgatggta taataattat ttttcttcct atgcggtcag ctacaatgcc
     8581 caacagagga gtcaacacta tccctggcat ggtaaaaact gttataacca gtgtcactga
     8641 tgaataggga atagcaaagt actgtgccat tgcagggagt gctggtgtaa tattggtaac
     8701 tcccattacc actaccagtg ttataccaaa agcaatgagc agatttgtat cagtgtagag
     8761 ttttttttgc tgttccatgg caatagtatt gttgtcattt tcccttcata gatagtataa
     8821 acataacatt aatagtacct catgatacgt atcagatacg tatcagggat tgttttaatt
     8881 caagcataaa acaacgggac aacagtaaac acagataaaa cagtataaaa aattttttga
     8941 ctggatgtaa aaaatcattg acagcagtat tttagttgta tcatgataaa tcaatagttt
     9001 aattaaatgt tataaaatgt aatacaaaaa tttgtatatt aaaattatac taaacaagtg
     9061 agaggaggat tggatggcac agttcaagtc agaatctatg tcagctgttt ttcaggagca
     9121 ggctgcaaag ttgggaagta aagcctgtgt atcatataaa aaaggcagtc agtgggtgga
     9181 tatatcctgg gccgatatga atactatggt acacaatatg gcttattatt taatggactt
     9241 tggaatcaag aaaggagaca gggttgcaat cttttcacca aatagatggg aatggtgggt
     9301 ggcaagttta gcaaccactt caattggggc ggtgagcgta ccaatttatg ccactaactc
     9361 agctgaagaa gcacagtatg tgctggaaca ttctggctct tctatttgct ttgttggtac
     9421 tgaagaccat gctgacaggg ttttaaaagt tatcaagaag tgccctaaac tggaccagct
     9481 agtaattttt gatgatcttg caaagaaaaa gcagaatgtt ataaccttta aagaagcact
     9541 ggaaaaaggt gcaaaaaagg caaaacctgc tgaatttgaa aaacgtttaa aagctatcaa
     9601 acgggatgat gtgtccacaa tcatctacac ttcagggact accggtaatc ctaaaggggt
     9661 aatgctttcg cattataatt tctactccaa tgctaaaaat atcatggctg atatgggcaa
     9721 gttttttaat actgaggatg tttggctatc atttttaccg ttatcacact cgcttgagat
     9781 gacatgtggc ttttatgggc caatatgggt tggcggaaaa actgcgtttg ctgaaagcat
     9841 tgaaaagttg ctggataact ttaaggaagt tcgtcccact gtcattatca gtgttccacg
     9901 aatttatgaa aaagttcatg ccggtattct ggcacaggtt gctgctgcac caggtttgaa
     9961 gaaagcaatt ttcaactttg cactcaacac ggctaagaag aatctgccgt atgtatgtag
    10021 agatcttcca cgtaaaggat tgtttgcaat acggtacaac attgctgata agctggtatt
    10081 ttccaagctt aaagctgcta ttggcatgga caggttgcgc tatgctatat ctggtggcgc
    10141 gccactttcc gtgtctgatg ctgaattctt tattggtatg ggaatgaaga tacttgaagg
    10201 ctttggatta accgaaacga cgccagtatt aacctataac cgaccatggt ttattaagcc
    10261 aggtacggta gggcaggcta ttcctgaaac caagataaag attgctgatg atggtgaaat
    10321 tctggcaaaa ggtccacaga ttatgttagg ctattataag aacaagactg ctactgaaga
    10381 ggttatgact aaagatgggt atttcaaaac tggtgatatt ggtgttattg atgaggatgg
    10441 tttcttgaag ataactggcc gaatcaaaga tattatcgtc acagctggtg ggaagaatat
    10501 atcacctcag aatattgaaa atagcgtaaa gacttctccg tatgttgagc agatagcggt
    10561 tattggcgat aaacgtaagt atttaagcgc acttgtaatt cctaactttg aagcattggg
    10621 aaaatgggcc aagcagaaag gaatcagctt cagtagcaat gcggatctca ttaaaaatga
    10681 tgaagtgaac aagctcattg aaagtgaaat tgcaaaatat accaagcagt tttcacgcgt
    10741 tgagcagatt aagaagttca cactccttga ggctgaatgg acacaggcaa caggtgaatt
    10801 aacaccaaca cagaaggtaa aacgaagaat tattgaacag aagtatgcta aagaaattga
    10861 ggctatgtat ccacctgata ttgagtaaat cctttatagt aagataagct tggaagaggg
    10921 gtggtcttag accgcccttc tttttttatg gaaaaatttt ttaaaaaagc ttgaagttat
    10981 tgtttaacaa tatctaaata taacaggata atacataaca atttatttga tttattttat
    11041 aagggggttc tatgattaaa aacgtcttaa aaattgtagg tattctgatt ctaattttta
    11101 ttataatttt attggtcaag gctataacta tttctccaaa gcaggttgag gtaaaagaag
    11161 ccatcccgta taaaattgac agtatgattg ttgcccagca tctttcagat gctatacaga
    11221 ttcaaactgt atccaatcaa gatcaggcaa aaacagatgt taaacagttt gagcaatttc
    11281 acaaattttt agcaaaaaca tatccggctt tacataaaaa gctaaaaaaa gaaattgttg
    11341 ggggatatgc acttctatat acctggaagg gctctgaccc ctcactcaaa ccaatgttac
    11401 ttatggcaca tcaggatgtg gtaccggttg aggagttaac gaaagaccaa tggaaatatc
    11461 cgggtttttc gggtacaata gcagatggtt ttatatgggg gaggggagcg ctggatatta
    11521 aaaatcagtt aatttccata atggaggcag ttgaattttt aataaataaa ggctttgtcc
    11581 cgaaacggac aatctatctg gcatttggtc atgatgaaga ggttggtggt gaaggtgcca
    11641 aagctatagc aaaactgcta aaacaacgta acgttcagct tgaatatgtt cttgatgaag
    11701 gtggggctat tatggagggc atgttgcctg gcgtagaaac ccctatagca ttggttggaa
    11761 ttgctgaaaa aggatattta accgtaaaac taactgtaaa aggtgaaggt gggcactcgt
    11821 ccatgccacc acatcacact gctttaggga ttttatcaaa agcactggtg gatgtagaag
    11881 ataatccgtt cccaaagaac tttgacagcc ccgcacggca gatgtttgaa tatgttgcac
    11941 ctcacatgaa gtttccatta cgtttgttgt ttgcaaatat gtggtgcttt aaaccactgt
    12001 taaaatggca atttgaaaaa tctgaatcaa ccaatgctat gatacgcacc accgctgctg
    12061 taaccatggc cggcgcaagt gaaaaagaaa atgtgctccc aactcaagcc tgggctatga
    12121 taaactgccg tatccttcct ggtgaaacca tggattcaac cattgcatat ttgaaaaaag
    12181 taattggcaa tgatgcagtt accattgagg caacaccgtg gtctaatgac ccttcgccag
    12241 tttcggatat acaggcagga tcgtttactt caatagcaac tatcgcccgg caaataaatc
    12301 ctgatgttat tgtggctcca tacctggtgc ttggtgctac tgattcacgt cactacaatg
    12361 cgttaaccaa tcagatatac cgattttcac cagttaagat gaattctgaa gatttaaaac
    12421 gtgttcatgg aataaatgaa agaatcagta tatctgatat gggtaaaagc gtaaaatttt
    12481 tcattgcatt aattgaaaaa actcatatgt agcatgtgtg gaaggtttgc acagattgaa
    12541 cctgtggatg tcattgcaca gcgtttgcag gttgaggaag tactggtaca acatacagca
    12601 ccgcgctaca atattagtcc gggggaagat attgtggcgc tggtgcaaac accaggcaaa
    12661 acgttaattg aaatacggtg gggcctgata ccgtactggg caaaggaacc ttcttcattt
    12721 aaaccattaa tcaatgctcg tgttgaaacg gttatacaga agcccagttt taaaagggct
    12781 ttttttaaca atagatgtat aatacctgca tccggtttct atgaatggaa aaaagaaggt
    12841 gccaaaaaga ttccttattt cattaccgtg gaggattttt ttggatttgc aggtattttt
    12901 gatagtgtaa cagttaatga taagcagcta ctcacatgtg caatactcac cactgctgcc
    12961 agtgaacaga tgagtattat tcacaaccgt atgcctgtca ttattcggca atctgaattt
    13021 gatcaatgga taaataaaga aacatcacca gaaatactaa caaagctcat gcaacctttc
    13081 aatgacaggc ttgtgatata tccggtatca acaaaggtaa ataatccgtc atataaagaa
    13141 cctgattgca tacatcctgt ttaattttta tcatgaagat tgtaacaatg gtgcatgaaa
    13201 tgaagttaca ctcatgctac ctaagatggt aaatttttac tttaatcttt atttcagttt
    13261 tctttggtaa gctgcgatag tatctgtgtc agttcattca tggtaaaagg ttttcgcagg
    13321 attgcatcaa agccatagtc cctgtaattg gctataattg gctcgttggt atatccggtt
    13381 gttgcaatga atttacagga agttttaaat ttttgtaatt cagtaactat ctcaactccg
    13441 cctttaccac ctttgatggt aagatcaaga atagcaatgc taaagggttt attctgcttt
    13501 atggcatctg caaaagcgtt gatggcatca tcaccattgc tgacggtgac tgcagtacac
    13561 ccctgcagtg caagcatttc attaagtgat tcagctatta atgcatcatc atccataatc
    13621 agtatatgtg atttagaagg tgtgcaatac gattcagtgg tttcatagac gcttgtttca
    13681 tttggtgggg ctactggcag atagatggta aacagtgttc cttcttttga tgaattaacg
    13741 gtaatgtgac catcatgctt tttcactatt gaatatgcaa ttgaaagccc caatccatgt
    13801 ccgccatctc gtgttgtaaa aaacgggtca aagatatacg gcatgatatc aggcggtatc
    13861 ccttcgccgg tatcatgaat agatattgca ataaagttgc ctttaactgg tatacctgga
    13921 tcatctagag ttgcattttt agcagtaatg gtaatggtgc cctgtccttt aattgcctga
    13981 cgggcgttaa gaatgatgtt atggattacc tgtgaaatct gggttgggtc aacaagtgca
    14041 ttatggagat tattttcaaa ctcaacattg cacttgatgg gagagccact gaggacaaat
    14101 tcagcagtat ccttaatgat attaaccaac gatgtggatg ttttaactgg tgcgcctcct
    14161 tttgaaaatg ccagtagctg tgttgtaagg tcacgtgcct tcatgatagc tttctgtgct
    14221 cgttctgaag cctttagcac cttttcatta ttgggagtta gttgttcaat aattgagata
    14281 ttccccaaaa ttgcactgag catattatta aaatcatgag ctatcccacc ggcaaatatg
    14341 ctcaaagatt catattttgc tgtttttatc agctcttctt catagcgaac catatcggtc
    14401 atatcacgta tgtcaacaat gcatccatca attctattgg caacatataa tggagtacaa
    14461 acgatatgtg tatgatatat atcaccatta tttctgataa cggtatgccg atagagagaa
    14521 acaggctttc ctgaagttag aacctgtgtt actggatcag cgtaaggagt accggttttt
    14581 gcattatata tgttgcaata ttctttgaat tgttccccta tcacatcatt gtgtaatcca
    14641 aagagtttga gtgcagcagc attaatactg gtaataattc cattttcatc ggtaaccact
    14701 atcccgtctg caatcgatac cagaatagcc tgaagccggg cagtttcctt ttgaagatta
    14761 atattactga ccagaagttc ctgttgtgtt tgttcaagct cttcattgat tgcagctaat
    14821 tcttcattca ttgcttctaa ttcttcgttg gttgctgata attcttcata ctggcgttgt
    14881 attttttctt ctgctttttt tgcatttatt attcccaatt tatggattga gaaaattaag
    14941 taagaaataa ctgcaacaaa ggtcaatgct aagcttgaat ccataaaata ctcaatagct
    15001 tcccatcgtt gtaaacctaa atgaaaccgt gagtacaaag taaaaataat aagtagcagc
    15061 atattagctg tataatagaa ccatattcct ctcctgcgtt caataaaaag aagcggggta
    15121 tatacaaggc tggcagcaac taatgttata ctatctaacc gttgcagtat ctgccctttt
    15181 tcaaagaaca taacaatcca cagcgctatc tgtacacttg caaaagatat atttgctgca
    15241 atttggaact tccctcttcg taacagtatt aatgaaa
//
LOCUS       DAORYL010000073        15122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_3155_length_15122_cov_14.205549, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000073 DAORYL010000000
VERSION     DAORYL010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15122
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3155_length_15122_cov_14.205549"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="PLV81_11905"
     CDS             complement(<1..782)
                     /locus_tag="PLV81_11905"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOM10845.1"
                     /translation="MVPTAYLFDPVYMDHDTGWGHPERPERLAAIDQRLRQQTYYKSL
                     IKVEPKIGAKRYIELVHSPEYIERVKQEIESGIHYLDSMDTAVCKRSYEVALFAVGGS
                     LNMCDTIMSGEAIRGFCAVRPPGHHAEYDYAAGFCIFNNIAIAAKYLQVEHGLKKIAI
                     VDWDVHHGNGTQHTFERDKTVLYISTHQYPHYPGTGARSERGKGEGEGYTLNFPMPAG
                     SGEPEYLDVFKNQIVPALMKFEPDIILISAGFDAHNADPLSY"
     gene            complement(871..1509)
                     /locus_tag="PLV81_11910"
     CDS             complement(871..1509)
                     /locus_tag="PLV81_11910"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOM10846.1"
                     /translation="MKRISFLIFSLIIMIIASSTVFAANSELNGTLGTSYADDPAKYG
                     LDISLHYNWLLDPYFVTGLESGFYWVKWDRKIGVKQEGSVYVDVKADTNAYLIPMMFN
                     AQVRLPNLKDKLYVTPFVTIGLGYSFMILHYSQPDFTDSNTGKSYNADSITKLFSGLS
                     WELFFGASLKPAADSKVDFYAEAGYRSLPLKSGDLELDMSGLVIRLGVRYPL"
     gene            1795..2418
                     /locus_tag="PLV81_11915"
     CDS             1795..2418
                     /locus_tag="PLV81_11915"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOM10847.1"
                     /translation="MKKLTVLSLFLFIISCTSVDKSVYIKDKQSLQKPIRIAVLTFVD
                     APKYPGSGVSIADALTNELVQISNWDLVERSQIEKILKEKSLDMTGLTDYQLTDIGKL
                     AKTDYIIVGSVSDYYYDRQFYIVPKTKIAFNARIINTQTGSIVGTLRYNRETNKNAWC
                     GCCLLGWYYLPVLLLTDQNINTDVSKAARDVVRAIEKDISGKRGCCI"
     gene            complement(2435..2605)
                     /locus_tag="PLV81_11920"
     CDS             complement(2435..2605)
                     /locus_tag="PLV81_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10848.1"
                     /translation="MMAQHVTAIEQSIEQLNNLLKGLAAVRTGLSEKLVKANVISHVS
                     VEGLGTIIDSYQ"
     gene            2782..2949
                     /locus_tag="PLV81_11925"
     CDS             2782..2949
                     /locus_tag="PLV81_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10849.1"
                     /translation="MGSNHKLAKMFATSPLKKEMRDDFIKKRLLQPIKAGNKVEINTF
                     YFQKETLHNEN"
     gene            2939..4759
                     /locus_tag="PLV81_11930"
     CDS             2939..4759
                     /locus_tag="PLV81_11930"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOM10850.1"
                     /translation="MKISNIYLAVYTFILFMILSVNYGYADTNITIHPDKKVLNVKPN
                     AKACAIIHIELPSKGYIYANPKGEGTGKATTAYAKPTQHIVPEKTRYLPGTLYISPLD
                     SKPVYIYNKHTALCIPFEVKTTHEGTYSLSITIEALYCNDVSCTPIQQTITQIINVNT
                     SAPEFDTSLCTMYKVPEAIIHTVNKKDLPGHFNVIDAGSTPTSSLFHAIMFGILAGFL
                     LNFMPCVLPVISLKILSFISYGNNKPGQVRIMGLLFTAGILTSFLALASLAAFAGYNW
                     GELFQHKMFIIIMTAIIFALALSFFGVYTFNIPRLPQLQSANIYVDSYFKGIVATLLA
                     TPCSGPFLGATLAWSLTQHPFIIFMVFINIGLGMSLPYLLLSFYPSLIRFIPKPGDWM
                     ITFEQFMGFLMIATTMYLITLLDASTLTKTIWFLLLVSIGLWQWGRFGNITKPSIQRI
                     ISFVVLVLLVVSASMIFSSKKTSQAEITYKPYDWEAIVHNNTSQVAMVIFTAQWCPNC
                     KFVEATALTSSDVIEFIHKNNIAVYKADITTKNPPAETILQQLGSRSIPFVAIFPSGD
                     NFSQPVILRDIYTAKDLLKALQKAIELSKQSEFPQLLTIP"
     gene            complement(5232..6482)
                     /gene="purD"
                     /locus_tag="PLV81_11935"
     CDS             complement(5232..6482)
                     /gene="purD"
                     /locus_tag="PLV81_11935"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOM10851.1"
                     /translation="MRYLVVGSGGREHAIAWRLIHDGSASEVYVAPGNGGIDERFRVQ
                     VAVNDFESLYTICKQKNIDIVIIGPEAPLVDGVVDFLQNRGIPVFGPTAQAAQLEGSK
                     LFAKYIMQKYNVPTAHFREFKGKESLIGYIESSAVFPVVIKLDGLAAGKGVGVCRDRE
                     TALQFIHEMVKDDAKVFVEDFCDGEEASVLGISDGTTVLPFIAAQDHKRVYDNDEGPN
                     TGGMGAYAPAPVVTSRILERVHREILQPVILGMKNEGMPFVGILYAGLIIKGHDIKVL
                     EFNVRFGDPETQVLLPLINGKLGDFIQAAISGKLSSMNLSFRNKHAITVVLASGGYPG
                     SYKTGLPITGLEQLPEDILVFHAGTRRDGQLLTSGGRVLNITAIADSLEDAYDKVYNA
                     IPSVSFEGMHYRKDIGFRALKKRP"
     gene            6658..7533
                     /locus_tag="PLV81_11940"
     CDS             6658..7533
                     /locus_tag="PLV81_11940"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOM10852.1"
                     /translation="MRLSNEAKVGLLVMTSFTLFIVLVGLLTKFNISQKGYRLHVYFG
                     FLNDLRVGAPVKIGGGIKIGYVDEIRQTGEKTDVILWIDKGYRLSKAATFSIFTSGMI
                     GEKYINVVIPPMSEDVGFLNDGDIKYGIDPASFDQMMQTFQSFMQDKSGAQILAEIFQ
                     NSNKFVANLNKMVDENRYDVRQSVGTARAAIVTFSQQLQQFMNQMNVISKNMAYISEK
                     NREDITITLQNLSELTSSMNKIAYRLEKGRGTMGKLLYDEEIYTNIRDASISAKELFS
                     RLEKDPSLLLFKQKK"
     gene            7536..8885
                     /gene="radA"
                     /locus_tag="PLV81_11945"
     CDS             7536..8885
                     /gene="radA"
                     /locus_tag="PLV81_11945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOM10853.1"
                     /translation="MPKKPKTIYICNECGATFQKWLGKCPECNTWNTIVEEMQDTPVF
                     KNEIPFENSIHPLSQVLSESFQRIPTGIGEFDLVCGGGVVPGSIILLGGEPGIGKSTL
                     ALQVAQHCTTLYCSGEETLQQIHLRSRRLNIKPDSIHLTSVTDIDHIEALIRTNAPQL
                     VIIDSIQTLYSKDITSPVGSVSQIRYTASRLADIAKQTQTSILLIGHITKDGSIAGPK
                     ILEHIVDTVLYFEGDFTRDYRILRAFKNRFGSINEIALFTMTATGLTEVKDKNTLFVQ
                     SHDASFPGSVISATIEGTRTILFEAQSLVTATSFSNPRRMADGFDVNRLNLLVAVIEK
                     HAKISLSSFDIFINVAGGFTIDDTSCDLAIAAAIISSLQNIVIPHSIGIIGELSLSGQ
                     VRSASHSLRRLIEFVNSGITTILLPRNNVSEISNTGFTGTLIPLTTIAELTQILQRL"
     gene            9021..10517
                     /gene="guaB"
                     /locus_tag="PLV81_11950"
     CDS             9021..10517
                     /gene="guaB"
                     /locus_tag="PLV81_11950"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOM10854.1"
                     /translation="MKPKDGYSGEELLNSTQGLSYNDFIILPGYIDFNPDDVDLETQF
                     TRNLKLKRPLVSSPMDTVTESKMAISLALLGGIGIIHYNNTIEEQVAQVRKVKRFENG
                     FITDPVVLSPEHTIAHIDEIKEKFGFSGIPITVDGKLGSKLIGIVTNRDIDFETDRSK
                     KLKDVMTTDLVTAKVGITLAQANELLKKSKKGKLPIVDDEGKLVALMSRNDLVTNREY
                     PFASKDTNKQLMVGAAISTKDESKERLQELVKAGVNVVVIDAAQGNSIYQIEMIKYIK
                     KKYPELDVIGGNVVTIEQCENLIKAGADALRIGMGPGSICTTQVTMAVGRAQSTAVYR
                     CSKFAKQYNIPTIADGGIATIGHIAKALALGASTAMMGSMFAGTEEAPGEYFYENGVR
                     LKRYRGMASLEAMEKGGSKRYFAEDSKILVAQGVSGAVVDKGSMFDYVPYLVQGLKHA
                     FQDMGVRTIKELHEKLYKEELRFELRSLSAQIEGGVHTIYSYKEPKYL"
     gene            complement(10521..11180)
                     /locus_tag="PLV81_11955"
     CDS             complement(10521..11180)
                     /locus_tag="PLV81_11955"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOM10855.1"
                     /translation="MGIDGVIFDLDGTLLDTIADLAYCVNNVLLRYGYRTHPVEAYKQ
                     FIGDGMAMLIQRAVGETCSDKEITKLVFELKDEYAKNWNRETKPYPGVHDLLKELSHK
                     KIKISVFSNKSHEFTVEMVKYYFSDIAFSTILGLQDSIPRKPDPHGALLIAHTMQLEP
                     SQIAMIGDSATDVEMAHACGMYSIGVSWGYRPVALLLQHHPHAIANTPGDIIRIITSV
                     T"
     gene            complement(11186..11959)
                     /locus_tag="PLV81_11960"
     CDS             complement(11186..11959)
                     /locus_tag="PLV81_11960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOM10856.1"
                     /translation="MDLNNKCILLTGASSGIGKALLEELTQYNATIVVGDLNPEAIAH
                     NKNIKAVCCDVSKQKDIEMLFKKALSIMGEIDICIANAGFAYYEKLQKADYDHIHKIV
                     SVNYIAPIYFLEKMMEINKGKEACMVFTASAMAKLPLPGYALYSATKAALDGFAYSFN
                     FEKDRNFHLVVVYPIATKTEFFKTAGKGTPVPFPTQTPQKVAQEVVKGILKNKRYVFP
                     SKIFRGMMIVNRILPFALWCYAKVEQYKFKKWVASSESK"
     gene            12118..12723
                     /locus_tag="PLV81_11965"
     CDS             12118..12723
                     /locus_tag="PLV81_11965"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOM10857.1"
                     /translation="MIKPLKTIEKLKNGQAVTIVALGDSLTYGWMVDKGYIDYFHDFL
                     QSQYPNASIKIINKGIPGDTADGGLHRVSYDVISYNPDCVLIQFALNDYACGYSPHVF
                     GNYIRAIINAIQARLPSDIVLVTSVWFGDFPEAKNAYKLYDTILSIADDYKLPVSKTH
                     EYWKNAVNKGTPLQELVQFDGVHPTEEGYYIMARALQDLFS"
     gene            complement(12716..13990)
                     /locus_tag="PLV81_11970"
     CDS             complement(12716..13990)
                     /locus_tag="PLV81_11970"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOM10858.1"
                     /translation="MKLLIKYGRLIDPASNTDEEFDIFIADGKIVKIDKNIKKDDAQF
                     INAKDCIVVPGLVDMHVHFREPGREDVETIIGGSRVAAKSGYTSVCTMPNTNPVIDNQ
                     ALVRYIKKQAESGPINVFPVAAITKGSMGEELTEMGELVEGGAIAFSDDGKPVMNSTT
                     MRRALEYSRMFNVPIITHAEDITLSSGGIMNEGNNSIFLGLKGIPREAEEIMIARDVL
                     LTRLTKGRLHVAHVSSKGSLDIITMAKRNNIQVTCETAPHYFSLTDDAIAEHLSMAKM
                     NPPLRTEEDRKAIIEALRNGLIEVIATDHAPHLPNEKMQEIAFAPFGIIGLETAVPLI
                     ITVLIKGNGFNFIQAFEKVTVNPCKILGIDRGYIKEGGVADITIINPDKKINVNDDFI
                     ISRCKNTPFMGRELFGQVEYTICNGIIVYPFS"
     gene            complement(13990..14937)
                     /locus_tag="PLV81_11975"
     CDS             complement(13990..14937)
                     /locus_tag="PLV81_11975"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOM10859.1"
                     /translation="MSGLQRKDLLDIQSLATEEILLICNSAKYFKALFTRSIKTVPVL
                     RGKTVCLLFYEPSTRTRISFELAAKRLSADLINIAVQTSSVVKGESLIDTVHTLEAYK
                     ADFIVMRHAASLAPHFLSRNIKSSVINAGDGNHAHPTQALLDAYSLMEIVGSIKGLHI
                     GIVGDILHSRVARSDIEIFKRLGAKVSLIGPPTLVPDEFKMYGVDIYYEFDEILPELD
                     VINMLRIQRERQQGSFFPSIREYHKRFALTNERLKRCKKDVIVMHPGPINRGIEIDDE
                     VADSSHSIINEQVTNGVAVRMAIFYLLAGGTPLDEKEVE"
BASE COUNT         4695 a         3121 c         2667 g         4639 t
ORIGIN      
        1 atgtatgata aggggtcggc attgtgagca tcaaaaccag ctgaaataag gataatatca
       61 ggttcaaatt tcattaatgc aggaactatc tgattcttaa acacatctaa atattcaggt
      121 tcaccactac ctgccggcat cgggaaattc agtgtatatc cttcgccctc acctttaccc
      181 cgttcacttc gagcgccggt tccaggataa tgaggatact gatgtgtact tatgtacagt
      241 acagttttat cacgctcaaa ggtgtgctgc gttccattac catgatgtac atcccaatca
      301 actattgcaa ttttcttcaa cccatgctct acctgtaaat atttagctgc aatggcaata
      361 ttattgaata tacaaaatcc tgcagcataa tcatactccg catgatgacc gggcggtcgt
      421 actgcacaaa aaccgcgtat tgcttccccc gacattatag tatcacacat gttaaggctg
      481 ccacccaccg caaatagcgc aacttcatac gatcgcttgc atacggccgt atccatagaa
      541 tctaaataat gtatacctga ttctatctcc tgctttacgc gttcaatgta ttccggagag
      601 tgcaccagtt ctatataccg ctttgctcca atttttggtt caactttaat cagggattta
      661 taatatgtct gctgacgcaa tcgctgatct atagctgcca gccgctcagg tctttccgga
      721 tgaccccatc ctgtatcgtg gtccatatat acaggatcaa aaaggtatgc agtaggtacc
      781 ataaacaccc cgcatattca ataattgttt tacaaaacca aataaaatgt tttataaaaa
      841 gtcaagtact ttaaaaaata ggttttacaa tcacagagga tatctaaccc ctaaccgtat
      901 taccagccct gacatatcca gttctaaatc gccgcttttt agaggtaaac tgcgatatcc
      961 agcttcagca taaaaatcaa cctttgaatc agcagccggc tttaacgaag ctccaaaaaa
     1021 taattcccaa gatagtccgc taaaaagttt ggtgatagaa tccgcattat atgatttacc
     1081 agtatttgaa tcagtaaaat caggctgtga ataatgcaat atcatgaatg aatagcccag
     1141 gccaatggtt acaaaaggtg ttacatacag cttatccttt aagtttggca ggcgcacctg
     1201 tgcgttgaac atcataggga taagataagc attggtatca gccttgacat ctacatacac
     1261 tgaaccttcc tgtttaacac caatctttct gtcccatttt acccagtaaa atccagattc
     1321 caaaccggta acaaagtatg ggtcaagaag ccagttgtaa tgtaaagaaa tatccagccc
     1381 atattttgca ggatcatcgg catacgatgt ccccagggta ccgttcaatt cggaattggc
     1441 agcaaataca gtagaacttg caataatcat aataattaaa ctaaagatta aaaatgatat
     1501 cctcttcata ttcatagttt ctcctgctgt taaatatttt tttaaccata ccttcagagt
     1561 tattgtctgt caacatcaat ttaatgtaaa ccattttaca attgcaatca tagtatttaa
     1621 atcatgtatc caatgtatta aactattcct gaaagatttt ttgcaatttt tttaaatatg
     1681 atctattatt aatccataaa aattattgat tatttaaatt atttatgtaa atcttgttcc
     1741 atcttttttt acataaccca aaatgccgta ctatattatt taaggaatat cattatgaaa
     1801 aaattaacag tgctttcatt atttcttttt attatttcat gtacatcagt agacaaatcg
     1861 gtatacataa aagataaaca gtcattgcaa aagcctatac gtatagcagt tttaaccttt
     1921 gtagacgccc caaaatatcc tggttcagga gtaagtattg ctgatgcatt gaccaatgaa
     1981 ctggtacaga tatctaactg ggatctggtg gaacgttcac agatagaaaa gattttaaaa
     2041 gaaaaatcac ttgatatgac tggattaacc gattatcagc ttaccgatat tggtaaatta
     2101 gcaaaaaccg actatatcat agtgggaagt gtttcagatt attattatga caggcaattt
     2161 tatattgtac caaaaacaaa gatagcattt aatgcccgta ttatcaatac ccaaacaggc
     2221 agtattgtag gaactttacg ctataaccgt gaaaccaata agaatgcatg gtgcggatgt
     2281 tgtcttttag gatggtatta tctgccagtg ctgttgttaa cggatcagaa tattaataca
     2341 gatgtaagca aagctgctcg tgatgtggta agggctatag aaaaggatat tagcggtaaa
     2401 agaggttgct gcatataatt attacatccc ctttttactg atagctatct ataatagttc
     2461 ccaggccttc aacactgaca tgtgaaatta cgtttgcttt taccagtttt tcgcttaatc
     2521 cagttcgtac tgctgccaga cctttaagca gattattcag ctgttctata ctttgttcaa
     2581 ttgctgttac atgttgtgcc atcatgacac cctcctttgt gtcaaacatt aatttctatt
     2641 tgcaatatcg actaaatctg ctatattctt aatacaatat ttttgtagaa atttttgtat
     2701 ttttttaaaa ttatcttccc atatattatt aacttaattc catagtataa cacaatactt
     2761 tgaaatgggt gtttttaagg gatgggaagt aaccacaaac tggcaaaaat gtttgcaact
     2821 tcacccctta aaaaggaaat gcgtgatgat tttatcaaaa aacggttgct acagcctatc
     2881 aaagcaggga ataaagttga aataaataca ttttattttc agaaggagac tcttcataat
     2941 gaaaattagc aatatatatt tagcagtgta tacgtttatt ttgtttatga ttttatctgt
     3001 aaattatgga tacgctgata ctaacattac gatccatcct gataagaaag tactgaatgt
     3061 aaaaccaaat gcaaaagcct gtgctatcat acacatagag ctaccttcaa aaggctatat
     3121 ctatgcaaac cccaaaggtg agggaactgg taaagctaca accgcgtatg caaaaccaac
     3181 gcaacacata gtacccgaaa aaacgcgtta tcttccaggg acattgtata tttcacctct
     3241 tgacagcaaa ccggtatata tttacaataa gcacactgca ctctgtatcc catttgaagt
     3301 aaaaactacg catgagggta cgtattcatt atcaatcact attgaggcat tatattgcaa
     3361 tgatgtatcc tgtaccccta tacagcaaac tatcacccag ataattaacg tcaatacatc
     3421 agcacctgaa tttgatactt cactatgtac tatgtataaa gtgcctgaag ctataattca
     3481 tacagtaaac aaaaaggatt tacccggtca ctttaatgta atagatgcag gcagtactcc
     3541 aacatcaagt ttatttcacg ctatcatgtt tggcattctt gcaggctttt tgcttaactt
     3601 tatgccctgt gtgcttcctg taattagtct taaaatttta agttttatat cctatggcaa
     3661 taacaaacca ggccaggtac gaatcatggg attgcttttt accgcaggga ttctcacatc
     3721 atttctggca ttagcatccc tggcagcatt tgctggttat aactggggag agctttttca
     3781 gcataaaatg tttattatta tcatgacagc aataattttt gcactggcat tatcgttttt
     3841 tggtgtgtat acatttaata ttccacgact tccacaattg caaagtgcca atatatacgt
     3901 agattcctat ttcaaaggca tagttgccac actacttgct accccctgta gcggtccatt
     3961 tttaggggct acactggcat ggtcattaac gcaacatcct tttataattt ttatggtttt
     4021 tattaacata ggccttggca tgtcactgcc atacctgctt ttatcattct atccatcact
     4081 tatcaggttc atccccaaac ctggtgattg gatgataaca tttgagcaat tcatgggatt
     4141 tttgatgatt gccaccacca tgtatcttat aacattactt gatgcaagca ctctaacaaa
     4201 aactatttgg tttttattac ttgtatccat tgggttatgg caatggggac ggtttgggaa
     4261 tataacaaaa ccatctatac aacgaataat ttcatttgtt gtacttgtgc tacttgttgt
     4321 ttcagcaagc atgatatttt cttccaaaaa aacgtctcaa gctgaaataa cctacaagcc
     4381 ttatgactgg gaggcgatag ttcataacaa tacatcacag gttgcaatgg tcatttttac
     4441 tgcacagtgg tgcccaaact gcaaatttgt tgaagcaaca gcattaacat cttcagatgt
     4501 tatcgaattt attcataaga ataatattgc tgtatacaaa gccgatatca ccacaaagaa
     4561 tcccccagca gagaccatac tacagcaact gggctcacga tcgataccct ttgtagctat
     4621 cttcccttca ggggataatt tttcacaacc tgttatcctg cgcgatattt atacagcaaa
     4681 ggatttattg aaggctttgc aaaaagccat tgaattaagc aaacaatcag agtttcctca
     4741 acttttaact atcccttaaa ggcatgataa tgcaatcact cagatactac cgtgaactta
     4801 tacaaaaaat tgatgcattt actgagtcta ttaattcaac gtatggcaac caattacatt
     4861 gcagggaggg ctgcagcagt tgttgtatac tggaaagtat aatgcctgtt gaacttgtgc
     4921 ccatagtaca atggtatgta acacaacctg aatcaataaa aactataatt aaacaagaag
     4981 actctcattc ctgtatattt ttgcataaca atagctgtat catctatcct gtaagacctg
     5041 taatttgcag aacccatgga tacccggttt taatacgtaa cagtgttgat atatgcccat
     5101 taaataatgg gataaacttt gagccacagt atattctcaa tttagagaac tgtaacacaa
     5161 tgcttgcagc tattaatatt ttattcatta aggaagtaca aattcctgca ttacaaacag
     5221 aacgcattaa cctacggacg ttttttcaaa gccctgaatc cgatatcctt tctatagtgc
     5281 ataccctcaa aggacactga tggtatagca ttatatactt tatcgtaggc gtcttccaaa
     5341 ctgtcagcta tcgctgtaat attaagcact cttcctccac tggtaagtaa ctgtccatcc
     5401 cgtctggtac ctgcatgaaa aacaaggata tcctcaggca actgttccaa tcctgtaatt
     5461 ggcaaacctg ttttatatga gccggggtag ccacctgaag caagtaccac tgtaattgca
     5521 tgcttatttc taaatgaaag attcatactg gaaagtttcc cacttatagc tgcctgtata
     5581 aaatcaccca atttgccgtt tatcagcggt aacaggacct gtgtttcggg atcaccaaag
     5641 cgtacattaa attccagtac tttaatatca tgtcctttaa ttattaaacc tgcatacagt
     5701 atcccaacaa aaggcatgcc ctcatttttc atgcccagaa tcaccggttg aagaatttcc
     5761 cggtgcaccc tttccagtat cctgctggta accactggag ccggtgcata tgcacccata
     5821 ccacctgtgt ttggcccttc atcattatca tatacccgct tatggtcctg ggcagcaata
     5881 aacggtaaca ctgttgtacc atcactgatc cccagaacag atgcttcttc accatcgcaa
     5941 aaatcctcaa caaaaacttt tgcatcatct ttaaccattt catgaataaa ctgcagtgca
     6001 gtctctctat cccggcacac tcccacaccc tttcccgcag caagcccatc taactttatt
     6061 actaccggaa atactgctga agattcaata tagcctatga gtgattcttt acctttaaat
     6121 tctctgaaat gagctgtcgg cacattatat ttttgcatga tatactttgc aaaaagcttg
     6181 ctcccttcta gttgggcagc ctgagctgtt ggaccaaata ctggtatacc tctattttgt
     6241 aaaaaatcaa ctactccatc aacaagagga gcttcaggcc caattataac aatatcaata
     6301 tttttttgtt tgcagatagt gtataatgat tcaaaatcat tcacggcaac ctgaacccgg
     6361 aatctttcat caataccacc attacctggt gcaacatata cttcactggc acttccatca
     6421 tgaatgagtc tccatgcaat ggcatgttca cgcccaccag accctactac taaatatctc
     6481 attgattcct cctgtcatat gctggtattg ttgtaaacaa gagatataat ttttcaatca
     6541 ttttatgtat acatctttca tgcgtacaat attttttata tattataaga ataattcttg
     6601 aacttacgtt atgtacagta tatgagataa caacaccctt atatgaggag gtttgctatg
     6661 cgcctgagta atgaagcaaa ggttgggctt ctggttatga caagctttac gctttttatt
     6721 gtactggttg ggctgttaac aaaatttaac atttcacaaa aaggataccg cttacatgta
     6781 tattttggat ttttaaacga ccttcgcgtt ggtgcccctg ttaaaattgg tggtggaatc
     6841 aagattggat atgtcgatga gatccgccag acaggggaaa aaactgacgt tattctgtgg
     6901 atagataagg gataccggtt atcaaaagca gcaacatttt caatctttac atcaggcatg
     6961 attggtgaaa aatatattaa tgttgtcatt cctcctatga gtgaggatgt cggttttctt
     7021 aacgacgggg atattaagta tggtattgac cccgcaagct ttgaccagat gatgcagaca
     7081 ttccagagct ttatgcagga taaaagtgga gcccagatcc tagcagagat atttcagaat
     7141 tcaaataaat ttgtggcaaa cttaaataag atggttgatg aaaaccgtta tgatgttcga
     7201 cagtcagttg gtaccgcacg tgcggctatc gtaacttttt cccagcaatt gcagcaattt
     7261 atgaatcaga tgaatgttat ttcaaaaaat atggcataca tctctgaaaa aaaccgtgaa
     7321 gatattacca ttacattaca aaatctttct gaactcacat ccagcatgaa taaaattgca
     7381 taccgtcttg aaaagggtcg tggaacaatg ggaaagctcc tctatgatga agaaatttat
     7441 accaacatac gtgatgcatc catttcagca aaagagcttt tttcaaggct tgaaaaagat
     7501 ccatcattac tactctttaa gcagaaaaaa taacaatgcc taaaaaacca aaaacaatat
     7561 atatctgcaa tgaatgcgga gcaacattcc agaaatggtt agggaaatgc ccggaatgca
     7621 atacctggaa tactatcgtt gaagaaatgc aggatactcc tgtttttaaa aacgaaatac
     7681 cctttgaaaa ttccatccat cccctttcgc aggtactatc agaatcattt caacgtatac
     7741 caacaggaat cggcgaattt gatctggtgt gtggtggagg agtagttccc ggctccatta
     7801 tccttctggg aggcgaacca ggtataggca aatccacatt agcattacag gtagctcagc
     7861 attgcacaac tctgtactgc tctggagaag aaactctgca acaaatacat ttgcgatcac
     7921 gccggcttaa tataaaacct gattcaatcc accttacctc agttactgat attgaccata
     7981 tcgaagcact catacgcact aacgcaccac agcttgtaat aatagactct atccagacac
     8041 tctattcaaa agatataact tcacctgttg gttccgtaag ccagattcgc tatactgctt
     8101 cacggcttgc tgatattgca aaacaaacgc aaacctctat actgctaatc gggcatatca
     8161 ccaaggatgg ctccattgca ggtccaaaaa tactggaaca tattgttgac acagtactct
     8221 actttgaggg ggattttacg cgtgactatc gcatattacg cgcctttaaa aaccgttttg
     8281 gctcaattaa tgagatagca ttatttacca tgacagccac aggattaaca gaagtaaagg
     8341 acaagaacac attatttgtt caatcgcatg atgcaagctt tccgggaagc gttatcagtg
     8401 ctactataga ggggaccaga accattctct ttgaagcaca atcgcttgtt actgcaacaa
     8461 gcttttcaaa ccctcgccga atggcagatg gttttgatgt taacaggctt aatttgcttg
     8521 tagcggtcat tgaaaagcat gcaaagattt cattaagctc atttgatata tttattaatg
     8581 ttgcaggcgg atttaccatt gatgatacat cgtgtgacct tgcaattgcc gctgctatta
     8641 tatcaagttt acaaaatata gttattccgc attcaatcgg catcattggc gaattatcac
     8701 tttctggaca ggttcgttct gcaagtcatt cattacgcag gcttatagaa tttgtaaatt
     8761 caggaataac caccattctg ttacctcgca acaatgtatc tgagatttca aatacaggct
     8821 ttacaggaac actcatacct ttaactacta tcgcagagct tacacaaatt ttacagcgcc
     8881 tttaattttt tcagttgatt attgaaacat tgtacgtaat acttaagcaa ataattgaaa
     8941 tatagtgcgc tccatgctat atcttatata acaccacaac actatagaaa atattattca
     9001 ttgactcata gaggtactac atgaaaccta aagacggcta ttcaggtgaa gagcttttaa
     9061 attccactca ggggttatcc tacaacgatt ttatcatatt accgggttat attgacttta
     9121 accctgacga tgttgattta gaaacacaat ttacccgcaa tcttaaactt aaacggccac
     9181 tggtatcaag ccccatggac actgtgactg aatcaaagat ggctatcagt ttggcattat
     9241 tggggggcat tgggatcatc cattacaaca ataccattga ggaacaggtt gctcaggtac
     9301 gaaaagtcaa acgatttgaa aatggattta ttactgatcc agttgtatta tctcccgaac
     9361 atactatcgc ccatattgat gaaattaagg aaaaatttgg attttcaggc atacccatta
     9421 ctgttgatgg caaattaggt tctaaactga taggtattgt taccaacagg gatatcgatt
     9481 ttgaaactga tagatcaaaa aaattaaaag atgtcatgac caccgatctt gtcactgcaa
     9541 aggttggtat tacactggca caggcaaacg agttacttaa aaaatccaaa aagggcaaat
     9601 tgccgatagt tgatgatgaa ggaaagctcg ttgccctcat gagccgcaat gaccttgtaa
     9661 ccaacagaga atatccattt gcatcaaaag atactaacaa gcagcttatg gttggggcag
     9721 ccatttccac caaggatgaa tcaaaagagc gattacagga acttgtaaaa gctggagtca
     9781 atgttgtggt catagatgca gcacagggta actctatcta ccagatagaa atgattaaat
     9841 atattaaaaa gaaataccca gaacttgatg tcattggagg taatgttgtc accattgagc
     9901 aatgcgaaaa cctgataaag gcaggggcag atgctctgcg cattggcatg gggccaggct
     9961 caatctgtac tacacaggtg actatggcag ttggccgcgc acaatcaacg gcagtgtatc
    10021 gatgcagcaa gtttgcaaaa caatacaata taccaacaat agccgacggc ggtattgcca
    10081 caatagggca catagccaag gcacttgcgc tgggtgcatc aactgcaatg atgggttcaa
    10141 tgtttgctgg caccgaagaa gcacctggcg aatattttta tgaaaatggc gttcgcctta
    10201 aacggtatcg tggcatggca tcactggaag caatggaaaa aggcggttca aagcgctatt
    10261 ttgcagaaga ctcaaagata ctcgttgcac agggagtttc tggcgcggta gttgataaag
    10321 gctcaatgtt tgattatgtg ccatatctgg tacagggatt gaaacacgct tttcaggata
    10381 tgggtgtacg cactattaaa gagcttcacg aaaagctata caaagaagag cttcgctttg
    10441 aattgcgttc gctgtcagcc cagatcgaag gtggtgtgca taccatttat tcgtataaag
    10501 aacccaagta tctgtaacct tcatgttact gatgtgataa tccgtataat gtctccgggc
    10561 gtattggcaa ttgcatgagg atgatgctgt aaaagcaacg caacaggtct gtacccccag
    10621 ctcactccta tactgtacat accacatgca tgagccattt caacatccgt agccgaatca
    10681 ccaatcatgg caatctgcga tggttctaat tgcattgtgt gggcaataag cagtgcgcca
    10741 tgcgggtcag gtttacgtgg tatggaatcc tgcaaaccta aaattgtgct gaatgcaata
    10801 tcagagaaat agtatttaac catttctaca gtaaactcat gcgatttatt tgaaaaaacg
    10861 gaaattttta tttttttgtg cgatagttcc tttaaaaggt catgcactcc tggatatggt
    10921 tttgtttccc tgttccagtt ttttgcatac tcatctttca attcgaaaac aagttttgta
    10981 atctctttat ctgaacaagt ttccccaacc gcacgctgaa tgagcattgc catgccatcg
    11041 ccaataaact gtttatatgc ctctaccgga tgtgtgcgat acccatagcg taacaggaca
    11101 ttattaacac agtatgccag atcagctata gtatccagaa gtgtcccatc caggtcaaat
    11161 atgacgccat caattcccat ggtaattatt tgctctctga tgatgcaacc cactttttaa
    11221 atttatactg ctcaacttta gcatagcacc acagggcaaa tggtagaatt ctgtttacaa
    11281 tcatcatgcc tctaaatatt tttgaaggga acacatatcg tttatttttc agtatccctt
    11341 taacaacttc ttgagcaact ttttgaggtg tttgcgtggg gaaaggaacc ggcgttcctt
    11401 tgcctgctgt tttaaaaaat tcagttttcg ttgctatggg ataaaccact acaaggtgaa
    11461 agtttctatc cttttcaaaa ttaaatgaat aagcaaaccc atctagagca gctttggtag
    11521 ctgaatataa tgcatatccc ggcaacggta gttttgccat tgccgaggca gtaaatacca
    11581 tacatgcttc tttaccttta tttatctcca tcatcttttc aagaaaataa attggggcaa
    11641 tataatttac tgaaactatt ttatggatat gatcgtagtc agctttttgc aatttttcat
    11701 aataggcaaa accagcattc gcaatgcaga tatctatttc ccccataatg gaaagtgcct
    11761 tcttaaacaa catttcaata tctttctgct tactaacatc acagcatact gcttttatat
    11821 ttttattgtg tgcaatagct tcagggttta gatcgccaac cacaattgtt gcattgtatt
    11881 gtgttaattc ttccagcaat gctttcccta tcccggatga tgcacctgtt aatagtatgc
    11941 atttattgtt tagatccatc ctgtacctct ctgcaagatt tttcaaattt atcttaaaat
    12001 atagtagaac tctaatattg caagaatatt tcattgctac agtaaaccca ttacaatttt
    12061 gctaattgac aattttcata cagtctttac aatctttgag gaatttgtgg tagtgtcatg
    12121 attaagccac tgaaaactat tgaaaaactt aaaaacggcc aggctgtcac catagttgct
    12181 ttaggtgatt cattaaccta tggatggatg gttgataagg gatatattga ttatttccat
    12241 gattttttac aatcacagta tccaaatgct tcaataaaaa taatcaataa aggtatacca
    12301 ggcgacacag ctgacggcgg gttgcatcgt gtttcgtatg atgtcatttc gtacaatccc
    12361 gattgtgtgc ttatacagtt tgcattgaat gattacgcat gtgggtattc accgcatgtt
    12421 tttggtaact acatacgggc aataatcaat gctattcagg cccgattgcc atctgatata
    12481 gtgctggtta cttctgtgtg gtttggggat ttccctgaag caaaaaatgc atataaatta
    12541 tacgatacaa tactatctat tgctgatgac tataagctgc ctgtatcaaa gacacatgaa
    12601 tactggaaaa acgctgttaa caagggcaca ccattacagg aacttgtaca gtttgatggt
    12661 gtacacccaa ccgaagaagg ctactatatc atggcacgtg cattacagga tttattcagc
    12721 taaacggata tactattata ccattgcaga tggtatattc aacctggcca aaaagctctc
    12781 ttcccataaa tggcgtattc ttacatcgcg aaataataaa atcgtcatta acattaattt
    12841 ttttatcagg attgataatc gtaatatcag caactccacc ttctttgata taccctctgt
    12901 caatgccaag aatcttacag ggattgacag taactttttc aaatgcctgt ataaagttaa
    12961 acccattccc cttaataagg acagtaataa ttaatggcac tgcagtttca agacctataa
    13021 ttccaaatgg tgcaaatgct atttcctgca tcttttcatt gggaagatga ggcgcatggt
    13081 ctgtggcaat gacttctatt aagccattgc gcaacgcttc aataattgcc tttctatctt
    13141 cttccgtacg caatggagga ttcatcttgg ccatggaaag atgttccgct atggcatcat
    13201 ccgtcagaga aaaataatgc ggtgctgtct cacacgtcac ctgaatgttg tttcgtttgg
    13261 ccatggtaat aatatccagt gaacctttag atgaaacatg agctacatgc aacctccctt
    13321 ttgtgaggcg tgtaagcaat acatcgcgcg caatcataat ttcttcagct tcacggggga
    13381 ttcctttcaa acccaaaaag attgaattgt ttccttcgtt catgatacca ccgcttgaca
    13441 atgtgatatc ctctgcatgc gttataatag ggacattaaa catccgcgaa tactctaatg
    13501 cacgtcgcat tgtagttgag ttcataacgg gtttgccatc atcgctgaat gctattgcac
    13561 caccttcaac cagctcaccc atctccgtta attcttcacc cattgaacct tttgttatgg
    13621 ctgcaactgg aaatacatta attggtccag attctgcctg tttttttatg tatcgcacca
    13681 gcgcctgatt atcaattaca ggatttgtat ttggcatggt acatacactg gtgtaccctg
    13741 atttagctgc aaccctggag ccacctataa tagtttcaac atcctcacgg ccgggctctc
    13801 tgaaatgtac atgcatatcc accagtccag gaaccacaat acaatccttt gcattaatga
    13861 actgtgcatc atccttttta atattcttat ctatttttac tattttgcca tctgcaataa
    13921 aaatatcaaa ttcttcatca gtgttagaag cagggtcaat taatcttcca tatttaatta
    13981 acaatttcat tactcaacct ctttctcatc caggggagtt ccacctgcaa gaagataaaa
    14041 aatagccatc ctcactgcta ccccattagt gacctgctca tttattattg aatgggaact
    14101 atcggccact tcatcgtcta tttcaatacc gcggttaatt ggtccaggat gcatcacaat
    14161 cacatctttc ttacatcgtt taagccgttc attagttaac gcaaagcgct tatggtattc
    14221 ccgtattgaa gggaaaaatg acccctgctg gcgttcacgt tgtattcgta acatgttaat
    14281 aacatcaagt tcaggaagta tttcatcaaa ctcataataa atatcaacac catacatctt
    14341 gaattcatca ggcaccagcg ttggggggcc aatcaggcta acctttgctc cgagccgctt
    14401 aaaaatctca atatcagaac gagcaacacg tgaatgaaga atatcaccca ctatacctat
    14461 gtgcaaccct ttgatactgc ccactatttc catcagtgaa tacgcatcaa gaagagcctg
    14521 cgttggatga gcatggttgc catctcctgc attaataacc gatgatttta tattacgaga
    14581 taaaaaatgc ggcgctaatg acgccgcatg tcgcataaca ataaaatctg ctttatacgc
    14641 ttccagcgta tgcacagtat caatcaggga ttccccttta accacacttg atgtttgcac
    14701 cgcaatattg attaaatctg ctgacagacg ttttgccgcc agctcaaaac ttatacgggt
    14761 gcgtgttgag ggctcataga ataaaaggca caccgtctta ccgcgtaaaa ctggcactgt
    14821 tttaattgac cgtgtaaaaa gggctttgaa atattttgca gaattacaaa ttaataaaat
    14881 ttcctcggta gctaatgatt gtatatcaag taagtctttc ctttgtaaac cactcatatc
    14941 ctgtattacc ttttcttgtt atatatcatc ccaaatggca taaataattt caataatttt
    15001 tttaaatata tttttgattg aactattata tatgtactgc acacttgtag cctgtgtgta
    15061 tattgtaaag aaggaggaat gataaaaaag tcaaaatagt tgcggtaaga cgaggatctg
    15121 tt
//
LOCUS       DAORYL010000074        14805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_3227_length_14805_cov_10.606644, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000074 DAORYL010000000
VERSION     DAORYL010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14805
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3227_length_14805_cov_10.606644"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..581
                     /locus_tag="PLV81_11980"
     CDS             117..581
                     /locus_tag="PLV81_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009362235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10860.1"
                     /translation="MATKEDLKEFATKDDVNAIRAEMATKEDLKEFATKDDVNAIRTE
                     MATKADLKAFATKEDLKNFATKEDFLEFEARLSSTVERIREGIRLSLFEIEKELTEIK
                     GKLRFYDFDYFSRQNDALLKLLKDLYEEKTFIVARVKDHENRLEAIEYKLGS"
     gene            710..1483
                     /locus_tag="PLV81_11985"
     CDS             710..1483
                     /locus_tag="PLV81_11985"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOM10861.1"
                     /translation="MKPKKQIIAPPVKAAAYFNKKSSETGILLLHGFTGTPAEMRYLA
                     EKLNESGATVYVPRYPGHGTSLIEMANSSVEAWFTAAREALFELKAHCNNVYIAGLSM
                     GGIFAILLAREFAIQKIALMSVPCTLKEKTIYAAPVAGLFTKILWVPNKTKGVCDEAA
                     RKEHICYDDGIPVRQSWQLFKTIKKAMKTLPYVDSQVLIVQSKNDQVIPEHSAEYIYS
                     RLGSEHKQIVWLSKSDHAITVDYEKDIVAKHCIEFFLQQ"
     gene            complement(1508..2827)
                     /locus_tag="PLV81_11990"
     CDS             complement(1508..2827)
                     /locus_tag="PLV81_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOM10862.1"
                     /translation="MTSYIEKYFDIQNNNTTISTEIRAGIATFLTMAYIIVVNPLILK
                     DAGMPFHAVLFATVMVSCISSMLMGLVANLPFALAPGMGINAFFTYTLVGSGTPWQVA
                     LGAVCISGIIFMLLSSWGIREKIVAAIPLHLRYAVAAGIGLFLGFIGLKNAGVIISHP
                     ATLVTFGGMGIQQLLFACGLIVTGVLVYRKIKGALIIGIIVTAALVWGTSHIYTLMGL
                     NPFAALPSEYSAYPDTSLFLNFNIRDALSVSLIVPIFTLLFTDMFDSISTFVGVAEVG
                     GFIDHKTGLPHNVGKALFIDGFATFISGIFGTSSATTYIESAAGVEEGGKTGLTAVVA
                     GLLFLPFMWLSPLLSFIPSVATAPVLVLIGLFMMQPLSHINWKDISQSLPAFVTCLMI
                     PLTYSITQGIVWGFLSYTFICLATGKFKELSPTLIIIDVFAGIALIV"
     gene            3636..4196
                     /locus_tag="PLV81_11995"
     CDS             3636..4196
                     /locus_tag="PLV81_11995"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOM10863.1"
                     /translation="MELQQAIVERRSIRRFTDYYVTDDEIKQILEAARMAPSWANVQP
                     WEFIVVRDKALIEKITETYSPNNPARKCSLASTALLVACARKNISGCKEGQQVTALPN
                     WYMFDVGMAVQNLCLKAHELGLGTVVVGFLDHQKCNEILNVPPDSTVVVTIPVGKPEV
                     SGKQGPPRKELTEFVYLDVYGNKFFK"
     gene            4205..4666
                     /locus_tag="PLV81_12000"
     CDS             4205..4666
                     /locus_tag="PLV81_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HOM10864.1"
                     /translation="MDIKDKWIYFAGPLFTAAERQFNELLSQYIEQAGYAVYLPQKEC
                     QGISSANDIFRHCIKGLDNAWCVVALVDGADTDSGTCFEMGYAYAKGIPIIGLRTDFR
                     KSADDGHVNLMLSQSVATLCFVNSLETISIEEIGYKLKDEISRMLVKVIKS"
     gene            4789..5412
                     /locus_tag="PLV81_12005"
     CDS             4789..5412
                     /locus_tag="PLV81_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10865.1"
                     /translation="MKKIVVYSAVFIGGIAFALLALLIYGFSFSQNVVIQDVTKQYYH
                     ASRIVVFGNVAIGFLSDSSSQNTDLDKLPHTFKIVTTGTIANMYFNPFESVYVPPQYL
                     GVYHINASGHEGILVLQVKDGVLYGSVRFPNWANGVWEPLKGVNIKNNTLWFTRSVTN
                     EQERIKTGAREYFTQQYVGTYKDNGKKIIGFYKLRGAKYLFEAYRKK"
     gene            complement(5417..6367)
                     /locus_tag="PLV81_12010"
     CDS             complement(5417..6367)
                     /locus_tag="PLV81_12010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOM10866.1"
                     /translation="MNGLDVLLSKVTIFKKLTQEEILQLVPFLSQKDYPEGSIIFDEG
                     TYGSELFIVASGEVAAFIKLPDGSLREVARFTTGDFFGDMAIFENDVRSATCIAQTDC
                     RLLSLTASNFFTIINSHPSAAIKIMYDMLTVISQRLMKTGSFLTEMIQWGEAARKRSI
                     IDEFTGIYNRSYFEDTITLKMTQEKPFCLIMVDLDYFRKINDQYSHDVGDRAILAIVD
                     VLKNTFRENDALCRYGGDEFAVIFDGNDVAHAHKLAEKARQNVNAIDCRHLGMDNFKL
                     SLSMGIAAFPTFDRDSIRAKADEALYSAKERGRNCVVIAQ"
     gene            complement(6364..7287)
                     /locus_tag="PLV81_12015"
     CDS             complement(6364..7287)
                     /locus_tag="PLV81_12015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOM10867.1"
                     /translation="MDSKEILKTVQLFSSLNDSELNIIQNYCRFKELKENEKLFDYDE
                     DFKLLCIVTAGSIAIYSNGSSNRLIARYLPNESFGELNLCGITLGNAQAVALEQSSVL
                     LFPSISFEDFITQHANIGAKVIYQLLSNISFRIRETNKLISQNYGWVSELKKAIYTDR
                     LTGLYNRQYCEELLKENREYSIAVIKPDKFKAINDTCGHKAGDATLIALAQTIRKASP
                     DAIHIRYRGDEFIVATPPDTDIQSLAQAILKAIGTINIASITSGLITSITASIGTGSG
                     VFCQRLIDSVYERMWSVYHKGGNSIAVEGDM"
     gene            complement(7620..7865)
                     /locus_tag="PLV81_12020"
     CDS             complement(7620..7865)
                     /locus_tag="PLV81_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10868.1"
                     /translation="MPLRKMKEAAKDIKIPKRKPTSKSTPSKTVHNEQLHDAIRLQAY
                     YNYLHRITHNTPGDQLSDWLEAEKYILNSRKRRKPSA"
     gene            8326..9192
                     /locus_tag="PLV81_12025"
     CDS             8326..9192
                     /locus_tag="PLV81_12025"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOM10869.1"
                     /translation="MQKIAINLHPDDEGSLKIISALIKKAHAKGVQVLLPHYPIIQKE
                     FPSLIAKNDMYTNVDIAIAIGGDGTFIRTARIFAASNVPILGVNRGRLGFLNEFLPQE
                     ALDYFDEILKGNFTYSKRKMLKANIIRDGEQYTAVDYLNDAVITKGSFSRPIRIRLEL
                     NDEFVTCYSGDGLIIATATGSTAYSLSAGGPIVLPDDESVFIVNPICPHTLAIRPMVV
                     PDSMVLSARVVTTIENLLLTVDGQEAIALQETDIVHFSRSSYVVHIINHPSRRYFDVL
                     RQKLGWGANNTE"
     gene            9196..10902
                     /gene="recN"
                     /locus_tag="PLV81_12030"
     CDS             9196..10902
                     /gene="recN"
                     /locus_tag="PLV81_12030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOM10870.1"
                     /translation="MLVELKIKNFILIDELSITFGKGLNILTGETGAGKSILIDALSG
                     VLGEKMSTDMIRSGYDRAVLEGVFDISNLPQVKQILEQSGIDNEDDTLILRREIYSNG
                     KGRCFANSVQIPLAKLKEIADYLVDIHGQNEHQNIVQVAKHRELLDNFGMLQPLVQNM
                     SQLHKQLQEIKEKIGSLQIDEREKARRIDYLTHAIGEIEQANLQPNEEEALKNESNVL
                     SNAEKLFNELNTVQELMQGDRGVVQSLKKMEMSLGHMAGFDPNLSGVLETVREAYYSL
                     EDAYATLRDYARSIDFSPERINQVEERLALISSLKKKYGDTIQDILSYAQNAKRELST
                     ITTTEEELDKLQQEYDVTLKQARDLALQLSEKRLEAAKKLEQMVTRELADLGMQGTVF
                     RVSIKREISPDGEIEANNKKYILYPHGLDRVEFLLSANEGEELRQLRKVASGGEMSRI
                     MLALKKCILDADIVDSLVFDEVDAGIGGKTAEVVGKKLKALAKQRQVLVITHLPQIAA
                     MSDNHYMVQKKSVNARTTTLVEKLSPDEKVNEVARMLAGEVVTDLSVKHAKELIELAS
                     KQ"
     gene            10880..11488
                     /locus_tag="PLV81_12035"
     CDS             10880..11488
                     /locus_tag="PLV81_12035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF039181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative metallopeptidase"
                     /protein_id="HOM10871.1"
                     /translation="MNWLQSNNMKSDSSINLSDFMTFLLYDIINATPLFSHIDMNHVL
                     VCSAYNKKVTGGGIFAKVVPMRFENGVPVKKFKGHWYAFPKIMNNGFEILYIVYFYIP
                     RYFDLPAYEKLNVAFHELYHISPKFNGDVRRMGRYKFSHGYSKEHFNNQFKDELSHYY
                     NRIKNTPLNTILEMNFAQISEHFETVYTRNMKVPRPKKIKYN"
     gene            complement(11485..11760)
                     /locus_tag="PLV81_12040"
     CDS             complement(11485..11760)
                     /locus_tag="PLV81_12040"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOM10872.1"
                     /translation="MAKRLILHGVVQGVFCRYYCSETAKRLGLHGSATNLYDGTVEVL
                     LDTNENSLVDSFINALKTNPYGIRFYGSISRVDVSDYQGTIRGDYRF"
     gene            complement(11838..13517)
                     /gene="nadB"
                     /locus_tag="PLV81_12045"
     CDS             complement(11838..13517)
                     /gene="nadB"
                     /locus_tag="PLV81_12045"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOM10873.1"
                     /translation="MSIEVFTSDFLVIGSGIAGLTFAIKASDLGTVHIVTKKKDFDSN
                     TNYAQGGIASVFDPHDSIESHIKDTLTAGAGLCNETAVRILAESGPQRVQELINWGAH
                     FSTRKNPNGSVSLDLGREGGHSYNRIVHAKDLTGQEVERALLDEIARRKNIRVFENHT
                     AVDLLTQHQLKLTGKQINSYNNIRCYGAYILDNTTGVVHLFTSPYTLLATGGIGQVYL
                     HTTNPAIATGDGIAMAYRAGALIADMEFIQFHPTSLYEPGHKERAFLISEAVRGEGAI
                     LLNSRGERFMEKVHPLKELAPRDIVARAIDMELKRLGDQCVYLDISFKDRDFLESRFP
                     TIYHYCLEAGIDISKEPIPVVPAAHYLCGGIVSDLNGRTSISNLYVSGESACTGVHGA
                     NRLASNSLLEGIVFSHRAFCHIKEIFAPDNKDALPQFPQWDKKGTFDLEEWILIQHNI
                     EDVKRVMWDYVGIVRSNRRLERAARRITLLEEEIQDYYRRSTITSRLVELRNLVTTAK
                     LIVQSAMLRKESRGLHYNTNYPQQDESLNGSYIISSQQQPLFMKISEISFT"
     gene            complement(13529..14308)
                     /locus_tag="PLV81_12050"
     CDS             complement(13529..14308)
                     /locus_tag="PLV81_12050"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOM10874.1"
                     /translation="MYIDSHAHFDLCADDHNTTADAIVSLMDENQVTSAVHVVIDYEG
                     LKWGVDFVSRYKNIYLALGIHPSSLADSSTLNQFTVTIEQYIKTIPEKIFGIGETGLD
                     YYRMRQPKAMQQESFYTQIQLAKKYNLPVIVHSRDAMDDTYAILKEQTPLKGIIHCFS
                     GDFNDAKKFINLGFYISFAGNVTYKTATNLQDAAQYVPLDRMLLETDAPFLTPVPHRG
                     KPNHPGLVIHTYHFIASLKKVALSNCIDNIAQNFSNLLNTQ"
BASE COUNT         4601 a         2657 c         3055 g         4492 t
ORIGIN      
        1 tcagaagagc gatggcaacg aaagaagagt tggatgcaat caaaaaagcg atggctacaa
       61 aagaggattt aaaggagttt gctacaaagg atgatataaa tgtcataaga gccgagatgg
      121 ctacaaaaga ggatttaaag gagtttgcta caaaggatga cgtaaatgct ataagagctg
      181 agatggctac aaaagaggat ttaaaggagt ttgctacaaa agatgatgta aatgccataa
      241 gaaccgagat ggctacaaaa gcggatttaa aagcctttgc tacaaaagaa gatctaaaaa
      301 attttgctac taaggaagat tttcttgagt ttgaagcgcg gttaagctca accgttgaac
      361 ggatacgtga aggaatacgc ctatcactct ttgaaattga aaaagaactt actgaaatta
      421 aaggtaaact tcgtttttat gattttgatt atttttcacg acaaaatgat gctctgttaa
      481 agttgcttaa agatctttat gaagaaaaaa cgtttattgt tgctcgtgtg aaagatcatg
      541 aaaataggtt agaagcaata gaatataaac tggggagttg atttcgttta acatcttcaa
      601 aaatgatatg ttcactctca ttggcttttg aagtgttttc atacaaacta ctcattcata
      661 ttgatgggta ttactaatgt tttaaaaatt ttataattgg tgcaacagaa tgaaaccaaa
      721 aaagcaaatt attgcaccgc cggtgaaagc agcagcatat tttaataaaa aatcttctga
      781 aacggggata ttgcttctgc atgggtttac cggtacacct gccgaaatgc gatatttagc
      841 tgaaaaactc aatgaatctg gtgcaactgt gtatgtgccg cgttatccag ggcatggaac
      901 aagccttatt gaaatggcca attcaagtgt ggaagcatgg tttactgcgg cacgtgaggc
      961 actgtttgaa ctaaaagcac actgtaacaa tgtatatatt gcagggcttt caatgggtgg
     1021 tatttttgca atacttttag cgcgtgagtt tgcaattcaa aaaattgcac ttatgtcggt
     1081 gccatgtacc ttgaaagaaa agaccatcta tgcagcgcca gttgcagggc tttttacaaa
     1141 aatactgtgg gttcccaaca aaacaaaagg cgtgtgcgat gaagctgcac gcaaagaaca
     1201 tatatgctat gatgatggca tccctgttcg ccagtcgtgg cagcttttta agactataaa
     1261 gaaagccatg aagactcttc cctatgttga ttcgcaggtg cttatcgtgc agtcaaaaaa
     1321 tgatcaggta atcccagagc attctgcgga gtatatctat tcgcgcctgg ggtcagagca
     1381 taagcagatt gtgtggcttt caaaaagtga tcatgccatt accgttgatt acgagaagga
     1441 tattgttgca aagcactgca ttgagttttt cttacagcaa tgatgaagta caacagtaac
     1501 tatctcccta aactatcagg gcaatacctg caaaaacatc aataataatg agtgtgggcg
     1561 ataattcctt gaattttccc gttgccaggc aaatgaacgt ataacttaag aagccccaca
     1621 caattccttg cgtgatgcta taggttagtg gaatcataag gcaggtaaca aatgcaggaa
     1681 ggctttgtga gatatccttc cagttgatat gcgacagagg ttgcatcata aaaagcccta
     1741 tcaaaaccag tactggcgct gttgccacag agggtatgaa tgaaagcagg gggcttagcc
     1801 acataaacgg taagaataac aaacctgcaa ccacagcggt taatcccgtt ttgcctcctt
     1861 cttctactcc tgcagcactt tcaatatagg tggttgcact ggatgttcca aaaattcccg
     1921 agataaatgt tgcaaatccg tcaataaaca atgctttgcc cacgttgtgc ggcagacctg
     1981 ttttatggtc aatgaagccg ccaacttcag caacgcctac aaacgtggag atgctgtcaa
     2041 acatgtcagt aaaaagaagc gtgaagatgg ggacaataag acttaccgat agcgcatcgc
     2101 gaatgttgaa gttcaggaaa agcgaagtat caggatatgc actgtactca gagggcaggg
     2161 ccgcaaatgg attaaggccc atcaaggtat agatatggct tgtaccccaa accagtgctg
     2221 ctgttacaat gatgccaata ataagtgctc ctttgatttt tcgatatacc agtacgccag
     2281 ttacaataag cccgcaggca aagaggagtt gctgtattcc catgccgcca aaagtaacca
     2341 gtgttgcagg atgtgaaata attacaccag cgtttttcaa tccaataaac cctaagaaaa
     2401 ggccaatgcc tgctgcaaca gcgtagcgca gatgtagtgg tattgctgct acaatctttt
     2461 cacgtatacc ccatgatgaa agcagcatga agataatacc tgatatacat acagcaccta
     2521 acgcaacctg ccagggtgtg ccgcttccaa ccagcgtata ggtaaaaaat gcgtttattc
     2581 ccatgcctgg tgccagtgca aaggggaggt tggccaccaa tcccatgagc attgaactaa
     2641 tacatgatac cataactgta gcaaaaagca ctgcatgaaa tggcatcccc gcatctttta
     2701 aaatgagcgg attaactacg atgatgtatg ccatggttaa aaatgttgca atgcccgcac
     2761 ggatttcggt gcttatggtt gtgttattgt tttgaatatc aaaatatttt tcaatatatg
     2821 atgtcataat ctccctcacg aaatatgata tcgtattggt taagattctt cctttagata
     2881 aatggttgct gagtttagtg aagcattcag tgtccaccct tcgattttca tcctaagtac
     2941 caattaaatt agtacaaagc atctactctg tcatcctgag ccctgatgaa atcggggcaa
     3001 aggatcttgt cttttgacac acagttattg caatagaact gaaactgacg ccctgcgttc
     3061 gcagtgacat gtaactaccg tcatcgttag ttacgataaa gtcgagataa tcaattttat
     3121 tttttactta acatttcata aattctattc aaacatgttg ttacaaaaca ctacaaagca
     3181 tatttgtaga tcctttctat tagctgcaca aacaccttgt gacaaattca aaacccagtc
     3241 gagcttcagt tgccaagctt attgaaacac gagtgttaac aatggcaata gagaagtttt
     3301 tttaatcctt tataaaagga atggggtagt tattaaattg caagaaaaat tttactaatt
     3361 gcattgtaaa cactacaata catgaagatg ttgctcatac ttcaattggt agtattaatt
     3421 atggataaat atactgacca actatgaaac taaaagagaa taaaaacgtt gggtaatgat
     3481 acagacatca tcattgattt taatgtaatt gaattatact ggaaaaatcg tatttataca
     3541 taattttata taaaataaaa ttgctttaca ttattattat ttttctgtag ctttgccata
     3601 caggctatta tttttttgta tataaagagg tggttatgga attacaacaa gcgattgtcg
     3661 aaagaaggag tattcgccgc tttaccgatt actatgtaac tgatgatgag attaaacaaa
     3721 tactggaggc tgcccgcatg gctccttcat gggctaatgt gcagccatgg gaattcattg
     3781 tagtgaggga taaagcatta attgaaaaaa taactgaaac ctactcaccc aataatcctg
     3841 ctcggaaatg ttcactggca tcaactgcgc tgctggttgc gtgtgcccgt aaaaatatat
     3901 cgggctgcaa ggaaggtcag caggtgactg ctctgccaaa ctggtatatg tttgatgttg
     3961 gtatggcggt tcaaaatttg tgccttaaag cacatgagct tgggcttggc acggtggttg
     4021 ttggatttct tgatcaccaa aagtgtaatg aaatccttaa tgttccacct gattcaacgg
     4081 ttgttgtaac aatcccagtt gggaaacctg aggtaagtgg caaacaggga ccaccacgta
     4141 aagaattaac tgaatttgtg tatttagatg tttatggcaa taaatttttc aagtaaggta
     4201 tatcatggat ataaaggata aatggattta ttttgcaggg ccactattta ctgcagcaga
     4261 aaggcagttt aatgaattgt tatctcaata tattgagcag gcgggttatg ctgtgtatct
     4321 accgcaaaaa gaatgccagg gaatttcttc agccaatgat atattcaggc actgcataaa
     4381 gggacttgat aatgcgtggt gtgttgttgc actggtggat ggtgccgata ctgattctgg
     4441 cacatgtttt gaaatgggat atgcgtatgc aaagggtatt cccataatag gtttgcgaac
     4501 ggatttccga aaaagtgctg atgatggaca tgtgaacctg atgttgtcgc aaagtgttgc
     4561 aacgttatgc tttgttaata gcctggaaac tatatctatt gaagaaattg gatataaact
     4621 gaaagacgaa atcagtagaa tgcttgttaa agtaattaaa agttaacatg ctacagtggt
     4681 ttatttgttg tggcttattt gtttttttag ctgtgatgtg cacatagata cctgatcacc
     4741 tatattctgc tttataagat acgaagtcaa tttctggagg gattaacagt gaaaaaaata
     4801 gtagtatata gtgccgtttt tattggcggg attgcttttg ctttactcgc attgctgata
     4861 tatggatttt cgttttcaca gaatgttgta atccaggatg ttacaaagca atactaccat
     4921 gcaagcagga ttgtggtgtt tggaaacgtc gcaattggtt ttttgagcga tagttcctca
     4981 caaaatacgg atcttgataa actacctcac acctttaaaa tagttactac aggaactatc
     5041 gccaacatgt attttaatcc gtttgaaagc gtgtatgtgc cgccacagta tctgggtgta
     5101 taccacatca atgcttcagg acatgaaggt atactggtgc ttcaggtaaa ggacggagtg
     5161 ttgtatggtt cggtgcgttt tcccaactgg gcaaatggtg tgtgggaacc attaaaagga
     5221 gtgaatataa aaaataatac cctgtggttt acacgttcgg tgaccaatga acaggaacgg
     5281 ataaagacag gtgcccgtga atattttacc cagcagtatg ttggtaccta taaggataat
     5341 ggcaaaaaaa ttattggttt ttataaatta cgtggtgcca aatatttatt tgaggcgtat
     5401 agaaaaaaat aaattatcat tgtgcaatca caacgcaatt acgaccacgt tcttttgcac
     5461 tatataatgc ctcatcagcc tttgcacgga tactatccct gtcaaatgtg gggaatgcag
     5521 caatgcccat actcaaggat agtttgaaat tgtccatgcc taaatggcgg caatctattg
     5581 cattaacgtt ttgccgtgct ttctcggcaa gtttgtgtgc gtgtgccaca tcattgccat
     5641 caaaaattac cgcaaactca tcaccaccat aacggcatag agcatcattt tctctgaatg
     5701 tattttttag tacgtcaact atagcaagta ttgccctgtc gccaacgtca tggctatact
     5761 gatcatttat ttttctgaaa taatcaagat ccaccatgat aagacaaaat ggcttttcct
     5821 gggtcatttt taatgtaata gtatcttcaa aataactgcg gttatatata cctgtaaatt
     5881 catcaataat tgaacgctta cgtgctgctt cgccccactg aatcatctcg gttaaaaatg
     5941 agccggtttt catgaggcgc tgcgatataa ccgtgagcat atcatacata attttgatag
     6001 cagcagatgg atggctattt atgattgtga aaaagttgga agctgtcaat gacaatagcc
     6061 tgcaatcggt ttgtgcaata caggttgcag agcgcacatc attttcaaat attgccatat
     6121 cgccaaagaa atcgcctgtg gtaaagcggg caacttcgcg aagactacca tctggtaatt
     6181 tgataaacgc agcaacctcg ccgctggcaa caatgaaaag ctcactacca taggttcctt
     6241 catcaaagat gatactgcct tcaggataat ctttttgtga aagaaacggt actagctgta
     6301 aaatttcttc ctgcgttagt tttttgaaaa tagtaacttt tgaaagcaat acatcaagcc
     6361 cgttcacatg tcaccttcca ctgctattga atttccacct ttatggtata ctgaccacat
     6421 acgctcatac acactgtcta taagcctctg gcaaaacact ccacttccag tgccaatact
     6481 tgccgttatt gatgtaatca gtccgctggt gattgatgca atatttattg tcccaatggc
     6541 tttcagtatt gcttgtgcca gtgattgaat atctgtatca gggggagtgg caacaataaa
     6601 ttcatcgccc ctgtaccgga tgtgaatggc atcgggggaa gctttgcgta tcgtctgtgc
     6661 cagagcaatg agggtggcat caccagcttt atgtccgcag gtatcgttga tggctttgaa
     6721 tttatcaggt ttaataacgg caattgagta ttccctgttt tcttttaaaa gttcttcaca
     6781 atactgtctg ttgtaaaggc ctgtaagcct gtcagtgtat atggcttttt taagttcgct
     6841 tacccagcca taattttgtg aaatgagttt gttcgtttcg cgtattctga atgatatgtt
     6901 cgatagtaac tggtaaatga cttttgcacc aatattggca tgctgggtaa taaaatcctc
     6961 aaatgaaatg ctggggaaaa gcagtacact ggattgttcc agtgctacag cctgtgcatt
     7021 gccaagtgta ataccgcaga gattaagttc gccaaaactt tcattgggaa gatatcgtgc
     7081 tatgagtcgg ttggaagagc cattggaata aattgcgatg ctaccagctg ttacaatgca
     7141 cagaagctta aaatcttcat cataatcaaa aagtttttca ttctccttaa gttctttgaa
     7201 tctgcagtaa ttctgaataa tgtttaattc gctatcatta agcgatgaga acaactgcac
     7261 agtttttaat atttcttttg agtccattat attctactat cgaacaaatt tacatgattc
     7321 atactatata taaccgtttc tagaggcggt ataatcatag tatgcataat acttagtaca
     7381 atgtttcaaa atgcagtatg taatgcaatt aaaaaaatga tatatattct attaagatca
     7441 ggaaaatgat aattgtgtga attcgatatt attataatac atacaataca tcaaagtgga
     7501 ttttgcattt ataaaagata acttttacaa tattatagca aaaaagcatg atgaatgaat
     7561 ttttttgtta ttgcaaaacg ctagatatca gtaatatcaa gggaagtagt attgttttat
     7621 tatgctgatg gttttcttct ttttctgcta ttaaggatat acttttctgc ttcaagccag
     7681 tcacttaact ggtcgccggg cgtattatgc gttattctgt gaagataatt atagtatgcc
     7741 tgtagccgta tagcatcatg taactgttca ttgtgtactg ttttactggg tgtgcttttt
     7801 gaggttggtt tacgtttggg tattttgatg tcttttgctg cttctttcat ttttcttaaa
     7861 ggcatatatg cacctccttt gaaaataaaa tgtattctcc tcaactacag tttctctggt
     7921 agctataaat gaaaaggacc tttaaacctt aacaaaataa gacaatacat ccggaataca
     7981 tctgggcaaa atttaaatat tttttattga aatgttaata ctatatcaaa tagtaaaata
     8041 ctaaaaagaa tatgatatgt caatacgtat tataaaaaga tagataacac catcctgtta
     8101 aatagtacac ttcaaagtac ttttagaaaa ttttgcttaa aaatgtatgc tatattttaa
     8161 ttgacataat tcagacaatt catatagtac agtggtattc aatcccttaa tactaatttt
     8221 attaatattg aataaacatc aaggctgtct ataaaaagtc gtttatggca tatctggtat
     8281 aaatgtattt tttaatttgt attttataat cattacagga aaacaatgca aaagatagca
     8341 ataaacctgc accctgatga tgaggggtca cttaaaatta taagcgcatt gattaaaaaa
     8401 gcacatgcaa aaggagtgca ggtgctacta ccccattatc ccattataca aaaagaattt
     8461 ccatctttaa ttgcaaagaa tgatatgtat accaatgttg acattgccat agctattggt
     8521 ggagatggaa cttttatccg taccgcgcgg atatttgcag catcaaatgt tcctatactt
     8581 ggtgttaatc gtgggaggtt agggttttta aatgaatttt tgcctcagga ggctctagat
     8641 tattttgatg aaattctaaa aggcaatttt acttattcaa aaagaaagat gctgaaggcc
     8701 aatattatcc gtgatggtga acagtacacg gcagtagatt atttgaatga tgcagtaatt
     8761 accaaaggat cattttcaag gccaattaga atacgactgg aactcaacga tgaatttgtt
     8821 acctgctatt cgggtgatgg ccttattatt gccacggcaa ccggttcaac agcatattca
     8881 ctttcagcgg gtggcccaat tgtccttcct gatgatgaat cggtatttat agtgaaccca
     8941 atttgccctc atacgcttgc tatacgtccc atggtggtac ccgattcaat ggtgttatca
     9001 gcacgggttg ttactactat agaaaattta ttattgacag ttgatggtca ggaagctatt
     9061 gctttgcagg aaacggatat tgtgcatttt tcacgttcat catatgtagt acatattatt
     9121 aatcacccat cacggcggta ttttgatgta ctacgccaga agttaggatg gggagcaaat
     9181 aatacagagt aggatatgct agtagaacta aaaataaaaa attttatcct tatagatgaa
     9241 ctgtcaataa cctttgggaa aggattgaat atcctcaccg gtgaaacggg tgcaggaaaa
     9301 tctattctca ttgatgcact ctccggggtg ctgggtgaaa aaatgtcaac tgacatgatt
     9361 cgttcgggct atgacagagc agtgcttgaa ggtgtctttg atataagcaa tttacctcag
     9421 gttaagcaga ttttagagca atcgggcatt gataatgaag atgatacgct aatccttcgt
     9481 cgtgaaattt attccaatgg caaaggaagg tgttttgcaa attccgtcca gattccactt
     9541 gctaaactaa aagaaattgc tgactattta gtggacatac atgggcagaa tgaacatcag
     9601 aatattgtgc aggttgcaaa gcatagagaa ctattggata attttggcat gttgcagccg
     9661 ctggtgcaaa acatgtcaca gcttcataag caattacagg aaataaaaga aaaaataggg
     9721 tccctgcaga tagatgaaag ggaaaaggcc cgccgtattg attatctaac tcatgcaatt
     9781 ggtgagattg aacaggcgaa tctacagcca aatgaagaag aggcattgaa aaatgaatca
     9841 aacgttttga gcaatgcaga gaaactgttt aatgagttga atacagtcca ggaactcatg
     9901 cagggtgatc gcggtgttgt ccagagctta aagaagatgg aaatgagttt gggtcacatg
     9961 gcaggttttg atccaaatct gtctggggtg ctggaaacag tgcgcgaagc atactattcg
    10021 cttgaagatg cgtatgctac gttgcgtgat tatgcacgtt caatagattt ttcacctgag
    10081 cgcattaacc aggttgaaga acggcttgca cttatctcaa gcttgaagaa aaaatatggt
    10141 gataccattc aggatatttt atcgtatgcg cagaatgcaa aacgcgaact ttctactatt
    10201 acaacaaccg aagaagagtt ggacaaactg caacaggaat atgatgtaac cttaaagcaa
    10261 gcgcgtgatt tagcattgca gctttcagaa aaacgtctgg aagcagcaaa aaaattagag
    10321 caaatggtta cccgggaatt agctgacctt gggatgcagg gaacagtgtt cagagtatca
    10381 ataaagcgtg aaataagccc tgatggggag atagaagcca ataataaaaa atatatttta
    10441 taccctcatg gccttgacag agttgagttt ttgctttcag caaacgaagg cgaggaattg
    10501 cggcagttgc gaaaagtggc aagcggtggt gagatgtcgc gcatcatgct tgcacttaaa
    10561 aaatgtatcc ttgatgcaga tattgttgat tcgctggtat ttgatgaagt tgatgcgggc
    10621 attggtggta aaacagcaga agtggttggt aaaaaattaa aagcactggc aaagcagcga
    10681 caggtgctgg ttattacgca tcttcctcag attgcagcaa tgtccgataa tcattatatg
    10741 gtgcaaaaaa aatccgttaa tgcccgtacc acgactcttg ttgagaaact atcgccagac
    10801 gaaaaagtaa acgaagtggc acgcatgctg gcaggcgagg ttgttaccga cctcagtgta
    10861 aagcatgcaa aagaattaat tgaactggct tcaaagcaat aatatgaaaa gcgatagttc
    10921 tattaacctt tctgatttca tgacgtttct tctttatgat ataattaatg ccacaccatt
    10981 gttttcccat atagacatga atcatgttct tgtatgcagt gcctataata aaaaggtcac
    11041 tgggggtgga atttttgcaa aggtagtacc catgcgcttt gaaaatggag tgccggtaaa
    11101 aaagtttaaa ggccactggt atgcgttccc aaagattatg aataacgggt ttgagatttt
    11161 gtatattgtg tatttttata tcccgcggta ttttgacctt ccagcttatg aaaaactcaa
    11221 tgtggcattt cacgaactgt atcacatcag tccaaagttt aacggtgatg tacgaagaat
    11281 gggcaggtat aaattttcac atggctattc aaaagaacac tttaataacc agtttaaaga
    11341 tgaactatcg cattattata atagaattaa aaatactccc ctcaatacaa tccttgagat
    11401 gaactttgca caaatttcag agcattttga aacagtgtat acccggaata tgaaggtgcc
    11461 gcgcccaaaa aaaattaagt ataattagaa gcgatagtca ccgcgaatgg ttccctggta
    11521 atcagaaaca tctactctgc tgattgatcc gtagaagcga atgccgtagg gatttgtttt
    11581 aagagcatta ataaatgaat caaccaatga attttcgttt gtatccagga ggacttcaac
    11641 ggtaccgtcg tacaagttgg ttgcacttcc atgaagtcct aaacgcttgg cggtttcact
    11701 gcaatagtaa cggcaaaaca caccctgcac aacaccatga agaataagtc gctttgccat
    11761 tgtgaaattc tccaaaaata agataattga tatagaggag catcgcaaag agcccctgca
    11821 ttgataacta ttcgatgtta tgtaaaagaa atctcagaaa ttttcataaa taatggttgt
    11881 tgctgtgatg atataatata acttccgttt aatgattcat cctgctgggg ataattggta
    11941 ttgtagtgaa gtccccggct ttctttgcga agcattgcac tctggactat cagttttgcg
    12001 gtggttacca aattgcgcaa ttcaaccaat cgtgaagtta tggtgcttct tctgtagtaa
    12061 tcctgtattt cttcttcaag cagggttata cgccgtgcag ccctttctaa tctgcgattt
    12121 gagcgtacaa taccaacata atcccacata acacgtttta catcttcaat gttatgctga
    12181 atcaaaatcc attcctctaa atcaaatgtt ccttttttat cccattgtgg aaactggggt
    12241 aaagcatctt tattgtcagg tgcaaagatt tcttttatat ggcagaatgc cctgtgggaa
    12301 aatacaatac cctcaagaag agaattgctg gcaagcctgt tggcaccgtg gacgccggtg
    12361 catgcggatt cacctgacac atatagattg ctgatagatg ttcttccgtt taaatcggat
    12421 acaattcctc cgcacagata gtgcgcagcc ggtaccaccg gaatgggttc tttgcttata
    12481 tcaatacctg cttccaggca gtaatgataa atagttggga aacgggattc aagaaaatcc
    12541 ctatctttaa aagatatatc aagatataca cattgatctc caaggcgctt taattccatg
    12601 tcaatagctc tggcaacgat atcccgtggt gccagctctt ttaaagggtg aaccttttcc
    12661 ataaaacgtt caccacggct attaagcagg atagctccct caccacgcac tgcctcactg
    12721 ataaggaatg cccgttcttt gtgccctggc tcatataaag atgttggatg aaattgaata
    12781 aattccatgt cggctatgag tgcacctgca cggtatgcca ttgcaatgcc atcgcccgta
    12841 gcaatggcag ggttggtggt atgaaggtac acctgaccaa tgccaccagt agcaagcaat
    12901 gtgtaaggag aagtaaaaag gtgaactact ccggttgtgt tatcaagtat gtatgcacca
    12961 tagcaccgga tattgttata agaatttatt tgtttacctg ttaattttag ctgatgctgg
    13021 gtaagcaggt ctactgctgt atggttttca aacacccgta tattttttct acgagcaatt
    13081 tcatcaagca atgctcgttc aacttcctgg cctgtaaggt cttttgcatg gacaatcctg
    13141 ttgtatgaat gtcctccctc tctgcccaaa tcaagagata ctgatccatt tgggtttttc
    13201 ctggtactga aatgcgctcc ccagtttata agttcctgaa cacgttgtgg gccgctttca
    13261 gccagtatcc gtactgctgt ttcattgcaa agacctgctc ctgcagttaa tgtatctttg
    13321 atgtgcgatt caattgaatc gtgtgggtca aaaactgaag caataccacc ctgcgcataa
    13381 tttgtatttg agtcaaagtc tttttttttg gttacaatat ggacagtacc caggtcagaa
    13441 gcttttattg caaatgttaa tcctgcaatg ccactgccaa tcactaaaaa atctgatgta
    13501 aaaacttcta tactcattac aaggtcaatt attgtgtgtt gagtaaattg gaaaagtttt
    13561 gtgcaatatt gtcaatacaa tttgataatg ccactttttt caatgacgca ataaaatggt
    13621 atgtatgtat aaccagtccc ggatgatttg gtttgcctcg gtgaggtaca ggagttaaaa
    13681 agggtgcatc ggtttccagt agcattctgt caagtgggac atactgtgct gcatcctgaa
    13741 gatttgttgc ggttttgtat gttacatttc ctgcaaaaga aatatagaaa cccaggttaa
    13801 tgaatttctt agcatcgtta aaatcaccag aaaagcagtg gataatgcct ttgaggggag
    13861 tttgctcttt taaaattgca taggtatcgt ccattgcatc gcgcgaatgt acaataacag
    13921 gcaggttata ctttttagct aactgtattt gagtatagaa cgattcctgt tgcatggctt
    13981 tcggctgacg catacggtaa tagtcaaggc ctgtttcacc aataccaaaa atcttttcag
    14041 gaattgtctt tatatactgt tcgatagtta ctgtgaattg gtttaatgtt gaactatcag
    14101 caagtgatga tggatggatt cctaacgcaa ggtaaatatt tttatacctg ctgacaaaat
    14161 caacacccca ctttagccct tcataatcga taactacatg cactgcagag gttacctgat
    14221 tttcatccat tagtgaaact atagcatctg ctgttgtgtt gtggtcatca gcacacagat
    14281 caaagtgtgc atgcgagtca atatacatac tatacctcaa atacataatt tgaggtatat
    14341 cctaaaacta ctattcattg atgcaatatc ttttttagtt acatgtatat tgcctgtgcg
    14401 tattttttaa cagtatcgca aatgaatgtt acattccctt ccctgtcgat agaatattta
    14461 tggttaattg aagtacatat tttgattatt ttcaggtaag aaatgctcta ttaaaggaga
    14521 tatcttctgt agcagcggta atacattccg taactttaca ctaaaaagta ttttcaggaa
    14581 ttattgcagc ttgaatttat tgtttacaat tatccattgg tgaataatga gtaaaatata
    14641 tacatggttg acaattttta ttaatacatg catagttgac ctgtgaggtt aatggcattt
    14701 tttcacgtat tcacagtaag cttgtcaggt gtactgagct aagcgaagaa tgcatcaaga
    14761 tgtgatcgtc gagtggttgc ccaggcaatc gtatcaagat gtggt
//
LOCUS       DAORYL010000075        14279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_3368_length_14279_cov_14.196639, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000075 DAORYL010000000
VERSION     DAORYL010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14279
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3368_length_14279_cov_14.196639"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..494)
                     /locus_tag="PLV81_12055"
     CDS             complement(117..494)
                     /locus_tag="PLV81_12055"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOM10875.1"
                     /translation="MTEKRQHPRKECNIKVKFDYFEGDPEKVTTSQKPTIKSKGYILN
                     ISASGAFMVCNCRVSVQMPVKLYCKINKTKLELTGEIVRTGMIQNNPSEVAQRYAAKK
                     VRGDSYVAIRFNELLPEEILSQV"
     gene            693..2321
                     /locus_tag="PLV81_12060"
     CDS             693..2321
                     /locus_tag="PLV81_12060"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOM10876.1"
                     /translation="MKDKKTKFIFVTGGVCSSLGKGISTASLGLLLEGHGYKLSIIKM
                     DPYINIDPGTMSPFQHGEVYVTEDGAETDLDLGYYERLTNAKLTRKNSVSTGQIYYEV
                     IMRERRGDYLGRTVQVIPHITNEIKKRIYDVANEDDLDFLLVEIGGTVGDIESIPFLE
                     AIRQIRQELGKTRVLNVHLTLVPTITVAGEQKTKPTQHSVKELMELGILPDILLCRSS
                     RPLTDDLKGKIALFCNVSERNVISAVDISHSIYEIPYMLHENKYDEVVLEHFGLPVKQ
                     LHLPEWDAFIKSLMNPKKKVTIAVVGKYISLQDSYRSIYEALTHGAVANNAELEIVRV
                     ESEEIERKYMGKLDELFKGVNGILIPGGFGNRGIEGKIATIEYARVNKIPLFGICLGL
                     QCAVIEFARHVCGMENANSTEFDPTTPYPVISLLEEQELIDKLGGTMRLGAYLCVFKD
                     GTNIKSIYNADSAMERHRHRFEFTLKYKQAFEDNGMVLSGFHPENNLVETIEYRDHPW
                     FICTQFHPEFKSKPVKPHPLFKDFIRASLENIRQ"
     gene            2427..3857
                     /gene="rpoN"
                     /locus_tag="PLV81_12065"
     CDS             2427..3857
                     /gene="rpoN"
                     /locus_tag="PLV81_12065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOM10877.1"
                     /translation="MAFLKLDVVQTQRLRLTQSLRQSLDLLQIPTLELEEILLQELEE
                     NPVLECDDTLDEGFESLDTVAKKEIEGISPKEEYTAEYESEDTPAFDYDKKNVMEIVS
                     QAETLQEHLLSQARMLQLAPDEMTMLEELITSLDDNGFLPASIEDLQKELGGDSSKIE
                     KLLSIIHTLDPVGCGATTVTDSLRIQAQYFYPDDVLLQQIIKDYLPHIQRYEYDKVAK
                     QLNISSDVVKEKAILLTSLTPYPGAHFFTRKPLYVRPDIEVQLIDDEVVINLLDDWMP
                     KLYINARYIEMLKQKKVDEKTREFIHDKVNAARNLINNISRRNETLLLVAYAIMNRQK
                     EFLQKGPGYLKPLIYADLESDLGFHESTIARAVANKYAQTRWGVFEFKYFFVKKIQSI
                     HNEDTSSDTIMKRMKELINNESKQNPLSDEEILDILKAEGIRIARRTIAKYRDMLSIP
                     ASHIRKKLYLMKHEGNHESNNYRQKF"
     gene            3826..4635
                     /gene="raiA"
                     /locus_tag="PLV81_12070"
     CDS             3826..4635
                     /gene="raiA"
                     /locus_tag="PLV81_12070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOM10878.1"
                     /translation="MNLTITGRNFEVSDSIREYAEKKLSKLTKYFHHLIDMHVWLLIE
                     RQDHIAEITINGDGVQFYAKEKAGDMYSSIDLLLDKIEQQIVRYKERHSGHKVTPLSE
                     LEDIATFTVKGEQSSITVRQVSNKPVDEKEAYLQMKLDGSDFTLFKKGEKSIKDSVDY
                     MNKNYAAIYKDGNEYTMVEIPFEMIQENSFNPSKFNEYTLEIKNDSSVNPDIKFKKKK
                     SCNVKMMAVNEAVIEITKNNTPFIVFFNTETNYLNIIYKHGKNLELLVPAF"
     gene            4643..5593
                     /gene="hprK"
                     /locus_tag="PLV81_12075"
     CDS             4643..5593
                     /gene="hprK"
                     /locus_tag="PLV81_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002155384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOM10879.1"
                     /translation="MKKQLRIRDILENTQKIDLQLTLITGREGIDKAITAIEINRPGL
                     TLAGFFDYFAYDRIQIFGLGETAYIKQLSRERREEIYAKFFSYDIVCCVYTHNEYPDE
                     IFIEYAKKHKVPTFVTQHPTTRFISLLTHIVEAAFAESVTIHGTLIDVFGIGILLIGK
                     SGVGKSETALELIERGHRLIADDLVEIKKIDESLLMGSGSELIRHHMEIRGIGILNVR
                     DIFGIRSVRNRKRIELVAELQEWDANTHYDRLGIEEQVYTILDIEVPYIIVPVRPGRN
                     IPIIIETAALNQRLKKMGVFSAQELDKKIQEWIARENNNK"
     gene            5590..5865
                     /locus_tag="PLV81_12080"
     CDS             5590..5865
                     /locus_tag="PLV81_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOM10880.1"
                     /translation="MKEKKVKVKSDAGVHARPASILVREAMKFPCDIYIIKGQMEANG
                     KSIMSVLGLAITKGSSLIIRANGEMEEEAVDRLSTLIENDFAELASK"
     gene            5862..6443
                     /locus_tag="PLV81_12085"
     CDS             5862..6443
                     /locus_tag="PLV81_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10881.1"
                     /translation="MKTHGIYCIIWLLIAFILVLQSPLQAQTKKKEAPSQELSLTDDE
                     IKVLFEKKKDPLFAGILSFYMPGLGQFYSDEKLKGALFLVTEYTIVIGSLFYFLDFSF
                     KAGSDSGFNLGIDAKRTDLGVISTQRKYLFYATVATLSLIHIYNIIDAVQSAGSFNTS
                     LQEKRQELIKKYPQLNFGYNGDGGMYFGLSSRF"
     gene            6421..6984
                     /locus_tag="PLV81_12090"
     CDS             6421..6984
                     /locus_tag="PLV81_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10882.1"
                     /translation="MGYRLVSKISLVAIIILSLFSIAFAQKDKQIQQNKESAVQSNGT
                     PIIFEETERKDPFIAGVLSWSWSGLGQFYTQNYKKGSAFLLADLAEKGVLVYMLFHIS
                     EKYSSSDQDVNWSDISKKDQTIVVGLLFSIFITRVFSVIDAVDSAHDYNRLIYYPYWK
                     SKQGFKTSFEPENKSLYISVSYPVNFK"
     gene            6981..7538
                     /locus_tag="PLV81_12095"
     CDS             6981..7538
                     /locus_tag="PLV81_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10883.1"
                     /translation="MKKLIITILLLLYSINVFAQDIIFGKVNFNSNNLNVFFSVTDIK
                     TNDIVEALKRGLEGQVEYTVQIVKDPLLPLMPKEIIKSITVKKKVKFDFFNKSYIVTQ
                     AKVPISFYSDESLIDELFFNRQIVIEDGFKYRKSNYLIRVRVTFTSVKLYFPLNIIFN
                     YVVGIWDFDTGWQYGPKLVGIPYSE"
     gene            7672..9447
                     /locus_tag="PLV81_12100"
     CDS             7672..9447
                     /locus_tag="PLV81_12100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM10884.1"
                     /translation="MMSFPIKDYLKEYVSKKNIKNSLIFIGAFFIFATIIAFLLPPQS
                     ENTSVGEGFFVNLVMIVPLAAAVFFILISFRLRVHPEVIYSSSIRTKIALALIVIATV
                     PTIPIIVITSNILNSTINQFFTEKTISALEKSVDLANQMVLEAEENLVQQLNQLKYDL
                     NNHYVSTEYLLHPAFSQRYMYAKTSLIVTLVETKGVLQNNVTLLHAGNNNPDIVSNIV
                     TFLQLAHIANITHCSRLSIFNNIYSVAYTQYGPYCIVMYRLIPKDMFATADFLNTAYS
                     EYKKQEFLKPYFQTGIGIALILLSLGIILLAIILSIMISQSITRPVLELVEASEKIAK
                     GDFQINLSRKEQDEIAALYESFNQMAKQLDHGRKMMYQTQKLQAWSDIARKLIHEIKN
                     PLTPIRLSAERLYRRYEENHPEFPIIVKDATGTIIEEVNILMNMLSEFSKFARLPEIN
                     LAEENINTILENCIDIYGNDAISFVTNLDTNLPPVMCDKALVRQAFLNIIQNSIEAKT
                     TEITITSIYDKEKQVIYILFKDNGVGIPEENLSKVFEPTFSTKESGMGLGLAIVEKII
                     IDHKWNITCDSKGDGTQFTITIPLQ"
     gene            9510..10865
                     /locus_tag="PLV81_12105"
     CDS             9510..10865
                     /locus_tag="PLV81_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOM10885.1"
                     /translation="MAKILVVDDEKNILNTMKAILQDEGHTVYTVENGNDAVQFIKSN
                     ECDVVFLDVWLPDIDGLEVLQRIKQIKQDVAVIMISGHGSIDIAVKSTRFGAYDFLEK
                     PPSMERVITSLKNALEQIELKRENILLKKNITLEDEMIGNSPPMQEVKRIIDTAASTN
                     ARVFITGENGTGKELVARAIYRKSKRSDKPFVKVNCAAIPDELIESELFGHEKGSFTG
                     AVARRLGKFELADKGTIFLDEICDMSPSAQAKVLRVLQEQQFERVGGNEVITVDVRVI
                     AATNVDVKEAIEQGRFREDLYYRLNVIPIYVPPLSERREDIPLLVDYFLEKFAREHGL
                     GVKQMNDSAMEFLVNYSWPGNVRELKNVIERLTIMVPSEVIQVQDVTKHIESYDYEDT
                     IAKETSSLKKAREQFEKDYIIKALKQYDKNISATAKALGIERTNLHRKIRQYHINIDR
                     L"
     gene            10916..11548
                     /locus_tag="PLV81_12110"
     CDS             10916..11548
                     /locus_tag="PLV81_12110"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HOM10886.1"
                     /translation="MYHLLKEAIVTHIQYGRRTPLVLAYTDEISYFSNRKNVAAPVEL
                     NTFQEKVQHAVAQCNKCKTTEKNIIHGGDASSGIMIILNPPSMLSSVEKKVLKPDVDT
                     MLDKMMKAIGVHVKACYVTHMIKCESQRELPGTMFSHCQNLLAKEIELVKPQIIIVMG
                     EMRPLQKIVKNSSGIQWFNIEHPITLLKNPELKKPAWNTLKLVKTVLDGK"
     gene            11551..13488
                     /gene="priA"
                     /locus_tag="PLV81_12115"
     CDS             11551..13488
                     /gene="priA"
                     /locus_tag="PLV81_12115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOM10887.1"
                     /translation="MFVDVAVGFPVDGFFTYSTDDTSITKGMRVVVNFNNRKTTGYVV
                     HVHDDPPNFDVKPVIKVLDTQPIFDDRLLRLAQFVSNHYVCYFGEALGTALPSGKSYN
                     IRTKPFTFGDSSKEVILTEEQEQIYRAIMNQPQKVHCIYGITGSGKTEVYIALAKKMI
                     QQGKAVLYLVPEISISSQMFERLKAVFGQTLVMYHSGMNPNQRLVSWKRFYTGQAMIA
                     VGTRSAIFMQAPNLGLIIIDEEHDSSYKENSTPRYHARTVAWYRHRQEGAMLVLGSAT
                     PSLETLYAIQRTSIVQHQLNKRFGTAKLPELEIVTVKASNARTIFSNTLLFSIKKAIE
                     AREQVILLLNRRGFAPVMLCQDCGTAVQCPHCSISLTHHKQKLLCHYCHYEAVVPEHC
                     SNCGSKHLVMVGSGTQRVEEVLQKMFPAATVVRLDQDSARRKNFLPAVVNDMMNGNID
                     ILLGTQMVAKGFDFPNVSLVGVMLADIGLLLPDFRAVERTFALLVQVAGRSGRGEVGG
                     KVIVQTINPDNPIFHFLKNHDYMGFCQKELSIRKALNYPPYIRLARLVVRGAKEDIVA
                     TVAGQCRDILQELSPQFPDITILGPSQAPLHKIASQYRYHIVLKSNNVEKLSGILAQC
                     KKKVNLQGTYVEIDIDPLDLL"
     gene            complement(13537..14091)
                     /locus_tag="PLV81_12120"
     CDS             complement(13537..14091)
                     /locus_tag="PLV81_12120"
                     /inference="COORDINATES: protein motif:HMM:NF018624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HOM10888.1"
                     /translation="MEAYEKTLSLKKNKGVASMQFPEIPDEIKKLLAQGEKIDEIRLD
                     AEGNWFHNGQPFTNQRIIDFFNKSINITKDGTYVIHYAQFTYPIVVEDAPIFVTGVRF
                     KGFADFETVILTLSTGQEEELDVSTLHVRKGKELYCYVRNNTMLAKFKRSASFTILDR
                     LEETDDIFYLTIAGKKIVLEEKLS"
BASE COUNT         4563 a         2385 c         2935 g         4396 t
ORIGIN      
        1 ccatacatgt taccatattt gtcaatatac cggttagcta aatcaaatgt acttattttt
       61 gatgtatcga tctctgatat atcgtctatg cggataaatc gtgtcttcat cagtgtttat
      121 acctgtgaca gtatttcctc aggtaacagt tcattaaagc gtatagcaac gtaactatcg
      181 cccctgactt tttttgctgc atatcgttgt gctacttcgg aaggattatt ttgaatcatt
      241 cctgtgcgta caatttcacc cgttaattcc agtttagttt tattaatttt acagtacaat
      301 tttaccggca tctgtacact gacgcggcag ttacatacca taaatgcccc ggatgcgctg
      361 atgttaagaa tatatccttt tgattttatt gttggtttct gacttgttgt aactttttct
      421 ggatcacctt caaaataatc aaacttgacc ttgatattgc attcctttcg tggatgttga
      481 cgtttttctg tcatggcatt tccctgtatt ataaaaattt ttttattttt tctttacaga
      541 acacaaaact gttactatag taacttcagt gttttgtcaa gaattgtttt ttttgttatc
      601 aattttgatt acatttttac aaattcttga caggtatctg taattactgt ttatggctta
      661 cgatatcatg gatactatct gttagaggat atatgaaaga caaaaaaacc aaattcattt
      721 ttgttaccgg tggagtatgc tcttcattgg gaaaaggtat atctacagct tcattgggat
      781 tgttacttga aggacatggg tataaacttt ccatcataaa gatggacccg tatattaata
      841 ttgaccctgg taccatgagc ccgttccagc atggtgaagt atatgttact gaggatggtg
      901 ccgaaactga tttagacctg ggatattatg agcgccttac caacgctaag cttacccgta
      961 aaaattctgt gtccacaggc cagatatact atgaagtgat catgcgtgaa cgccgtggtg
     1021 attatttggg caggacagtt caggttattc cacatattac caacgaaatt aaaaagagaa
     1081 tttatgatgt agcaaatgaa gatgaccttg attttttgct tgttgagatt ggtggaacag
     1141 ttggtgatat tgaatcaatt ccatttttag aagcaatacg gcagatacgg caggagttag
     1201 gtaaaacaag agtgttgaat gttcacctga cactggtgcc aacaataaca gttgctggcg
     1261 agcaaaagac aaagccaacg cagcattcag taaaagaact tatggaatta ggtatattgc
     1321 ctgatatact tctgtgccgt tcatcacggc cgcttactga tgatttgaaa ggcaaaatag
     1381 cattattctg taatgtctcg gaacgaaatg ttatctcggc tgttgatatc agccattcca
     1441 tttatgaaat cccatatatg cttcatgaaa ataaatatga tgaggtggtg cttgagcatt
     1501 ttggtcttcc ggttaaacag ttgcatcttc ctgaatggga tgcatttatt aaatccctga
     1561 tgaatccaaa gaaaaaggtc accattgctg ttgtagggaa atatatttcg ctgcaggatt
     1621 cataccgctc aatctatgaa gcgcttactc atggtgctgt tgccaataat gcagaacttg
     1681 aaatcgtacg ggttgaatct gaagagattg aacgcaaata catgggcaaa ctggatgaac
     1741 tttttaaagg ggttaacggc atattaatac ccggcggttt tgggaacaga ggtattgaag
     1801 gtaagatagc aactattgaa tatgcccgtg ttaataaaat tccgttattt ggcatttgcc
     1861 tgggattaca gtgtgctgtt attgagtttg cccgccacgt gtgtggtatg gaaaacgcaa
     1921 attccactga atttgaccct acaacgccct atcctgttat ttcgcttctg gaagagcagg
     1981 aacttattga taagcttggg ggtaccatgc ggctgggggc atatttatgt gtatttaaag
     2041 atggcacaaa tattaaatca atttacaatg ctgattcggc aatggaacgt cacagacacc
     2101 gttttgagtt tacccttaaa tataaacagg catttgagga taatggcatg gtgctgtcgg
     2161 gctttcatcc tgaaaataat ctggttgaaa ccatcgaata ccgggatcat ccgtggttca
     2221 tctgtactca atttcaccct gaattcaagt caaagcctgt aaagcctcat cctttgttta
     2281 aggattttat cagggcttcg cttgaaaata taaggcaata atgcaattta aatggacaat
     2341 tgggttgtaa tttttgtata tttatgtaag aatgtggttg tagtaaagga atatctgaat
     2401 tgaggaagat tgttgcgagg gagtgaatgg ctttcttaaa gcttgatgtg gtacaaaccc
     2461 agcgtttacg ccttacccag agtcttagac aatctttaga cttattacaa atacctacac
     2521 ttgaattaga ggaaatatta ttgcaggaac tggaagaaaa tcctgttctg gagtgtgacg
     2581 acacgcttga tgaaggattt gaatcccttg atactgttgc taaaaaggaa atagaaggga
     2641 tttcgccaaa ggaagaatat actgcagaat atgaaagcga agatactcct gcgttcgatt
     2701 atgacaaaaa gaatgtaatg gaaattgttt ctcaagccga gacactgcag gagcatttat
     2761 tgtctcaggc aaggatgctg caactggcac ctgatgaaat gaccatgctt gaagagctta
     2821 ttaccagcct tgatgataat ggatttttac ctgcttctat agaagatttg caaaaagaat
     2881 taggtggcga tagttcaaaa atagaaaaac ttctgagcat aatacacaca ctggatcctg
     2941 ttgggtgtgg tgctaccact gttaccgatt cattacgtat tcaggcacaa tacttttatc
     3001 ctgatgatgt cctgttgcaa cagatcataa aggattatct gccacatatt cagcgttatg
     3061 agtacgataa agttgcaaaa caactgaata taagtagcga tgtagtgaaa gagaaagcta
     3121 tactccttac cagtttgacg ccatatcctg gtgcacactt ttttacccgt aagccactgt
     3181 atgtacgccc tgatattgag gttcagctga ttgatgatga ggtcgttatc aaccttcttg
     3241 atgactggat gccaaaattg tatattaatg cccggtatat tgagatgctg aagcaaaaaa
     3301 aagttgatga aaaaacaaga gaatttatac atgataaagt taatgccgca agaaatctta
     3361 taaacaatat cagcaggcgg aacgaaacac ttcttcttgt tgcgtatgca ataatgaaca
     3421 ggcagaaaga gtttttacaa aaagggccag gctatttaaa acctttaatc tatgctgatt
     3481 tagagtctga tttaggattt catgaatcca ctattgcccg tgctgtggcc aataagtatg
     3541 ctcagaccag atggggagtt tttgagttta aatatttttt tgttaaaaaa attcaatcca
     3601 tacataatga ggatacatcc tctgatacca ttatgaaacg aatgaaggaa cttattaata
     3661 atgaatcaaa acaaaatcct ttaagtgatg aggaaatact tgacatattg aaggcagagg
     3721 gtatccgtat tgcgcgcagg actattgcaa aatacaggga tatgttatct atccctgcat
     3781 cacatatccg taaaaaacta tatttaatga aacatgaggg taaccatgaa tctaacaatt
     3841 acaggcagaa attttgaagt aagcgatagc attcgcgaat atgctgaaaa aaagttatca
     3901 aagttaacaa agtatttcca tcatttgatt gatatgcatg tatggttgct gatagaacgt
     3961 caggaccata tagctgaaat tactattaat ggcgatggcg tgcaatttta tgcaaaagaa
     4021 aaagctggtg atatgtattc ttctatagac ctcttgcttg acaagattga acagcaaata
     4081 gtacgataca aagaacgaca ttcaggacat aaggttactc cgttaagtga gcttgaagat
     4141 atagctacgt ttactgttaa gggcgagcag agttccatta cagtacgaca ggtcagcaat
     4201 aaaccggttg atgaaaagga agcatatctg cagatgaaac tggatggcag tgattttact
     4261 ttatttaaga agggtgagaa atcgattaaa gattcagttg attatatgaa taaaaattat
     4321 gctgcaattt ataaagatgg taatgagtac accatggttg aaataccgtt tgaaatgatt
     4381 caggaaaact catttaaccc atcaaagttt aatgaatata ctcttgagat aaaaaatgat
     4441 tcatcagtaa atcctgatat aaagtttaaa aagaaaaaat catgtaatgt taaaatgatg
     4501 gcagttaatg aagcagttat tgaaataaca aaaaacaaca ctccatttat tgtatttttt
     4561 aatactgaaa ctaactatct caatataatt tataaacacg gcaaaaattt agaacttctg
     4621 gtgccggcat tttaatggaa caatgaaaaa acaactgcgc atacgtgata ttttagaaaa
     4681 tacacagaag attgacctgc agcttacact gataaccggc agggaaggaa ttgataaggc
     4741 tatcactgca atagaaatta accggccagg tcttacactg gcgggttttt ttgattattt
     4801 tgcctacgac cgtattcaga tttttggttt gggtgaaaca gcatatatca agcagttatc
     4861 acgtgaaagg cgtgaggaaa tttacgctaa atttttttct tatgatatag tgtgctgtgt
     4921 atacacccat aatgaatatc ccgatgaaat ttttattgag tacgctaaaa agcacaaggt
     4981 cccaaccttt gtgacacaac atccaacgac ccggtttata agcttgttaa cccatatcgt
     5041 tgaagctgca tttgcagaat cagtaaccat acatggtaca ttgattgatg tatttggtat
     5101 aggaatattg cttattggta aaagtggtgt tgggaaaagt gaaactgctt tagagttaat
     5161 agaaagagga caccggctga ttgcagatga cctggttgag attaaaaaaa ttgatgagtc
     5221 attactgatg ggaagtggtt ctgagcttat acggcatcat atggagatac ggggaattgg
     5281 aatattaaat gttcgtgata tatttggtat caggtcagta aggaacagaa agcgtattga
     5341 gcttgttgcg gaattacagg agtgggatgc aaatacccac tacgacaggc ttggtataga
     5401 agagcaggtg tataccatcc ttgatattga agttccgtat attattgttc cagtaaggcc
     5461 cgggcggaat atcccgataa ttatagaaac tgcagcactt aatcaacggt taaaaaaaat
     5521 gggcgtgttt tccgcgcagg aacttgataa aaagattcag gagtggattg caagggagaa
     5581 taacaacaaa tgaaggaaaa gaaagttaag gtaaaaagtg atgctggtgt tcatgcacgt
     5641 cctgcatcta tactggtacg ggaggcgatg aaattcccat gtgatatcta tatcattaaa
     5701 ggccagatgg aagcaaacgg caaatccata atgtcagtgc tgggtctggc tataaccaag
     5761 ggaagttctc tgataataag ggctaatggc gaaatggaag aagaagctgt agaccgatta
     5821 tcaactctca ttgaaaatga ttttgcggag ttagcttcaa aatgaaaact catggtatat
     5881 attgtataat atggctgttg attgcattca tactggtatt acaaagccct cttcaagcgc
     5941 aaacaaagaa aaaagaagct ccttctcagg aactatcgct aactgatgat gaaatcaagg
     6001 tgctttttga aaaaaagaag gacccccttt ttgccggtat tctttctttt tatatgccag
     6061 gccttggcca attttatagt gacgaaaaat taaaaggtgc tcttttcctg gttactgaat
     6121 ataccatagt gataggatca cttttttatt ttcttgattt tagttttaaa gctggaagtg
     6181 attcaggttt taatttagga attgatgcaa agcgtactga tttaggtgtc atcagcactc
     6241 agcgaaaata tctgttttat gctacagttg ccacactatc actaattcat atttataaca
     6301 taattgatgc agttcaaagc gccggttcct ttaacacttc gttacaggaa aaacgacagg
     6361 aattgataaa aaagtatcca caacttaact ttggatataa tggtgatgga ggtatgtatt
     6421 ttgggttatc gtctcgtttc taagataagt ttggttgcaa ttatcatact atctttgttt
     6481 tcaattgcat ttgcccagaa ggataaacaa atacagcaaa ataaagaatc tgctgtgcaa
     6541 agtaatggta ctcctataat ttttgaagaa actgagcgaa aggacccatt catagcgggt
     6601 gtgctgtcgt ggtcatggag cgggttggga cagttctata ctcagaatta taaaaagggc
     6661 tcagcctttt tgcttgctga cctggctgaa aagggggtgc ttgtatatat gttatttcat
     6721 atttctgaaa agtattccag cagtgatcag gatgtaaact ggagtgatat cagcaaaaaa
     6781 gaccagacta ttgttgtggg gctccttttt tcaattttta ttacaagggt gttttctgtt
     6841 attgatgctg ttgattcagc tcatgactat aacagattga tttattatcc atactggaaa
     6901 agcaaacagg gatttaaaac ttcatttgag cctgaaaata aatcactata tataagtgta
     6961 tcgtatccag tgaattttaa atgaaaaaat taattataac tatattattg ttattatatt
     7021 ctatcaatgt ttttgcacag gatattattt ttgggaaagt taattttaat tccaataatc
     7081 tcaatgtgtt tttttcggtt actgatatta aaaccaatga tattgttgag gcattaaaac
     7141 gcgggcttga aggccaggta gaatatacag tccagattgt taaagatcct ttactaccgc
     7201 tgatgccaaa agaaattatc aaatccatta cagtgaagaa aaaagtaaaa tttgattttt
     7261 ttaataagtc ctacatagtt acacaggcaa aagttccaat ttccttttac agtgatgaat
     7321 cgcttattga tgagctcttt tttaacaggc agatagttat tgaagatggt tttaaataca
     7381 ggaaatcaaa ttatttaata cgtgtccgag ttacctttac ttcagttaag ttatatttcc
     7441 cattgaatat tatatttaat tatgttgttg gcatctggga ttttgatact ggatggcaat
     7501 atgggccaaa actggttgga ataccttact ctgaataata tactatgata gcaattattc
     7561 ctgaaaaata tggtatgaat aaaaaagcag tactcccgca ccgccaaaga tggacagagt
     7621 gtgcattaat attttgtttg taatttatat taaggaataa tttgtgatac catgatgtca
     7681 tttcccataa aagattattt aaaagaatat gtatcaaaga aaaatattaa aaattcactg
     7741 atttttattg gcgcattttt tatatttgca actatcattg cttttctttt gcctccccag
     7801 agtgaaaata cttcagttgg tgaaggtttt tttgtaaatc ttgtcatgat tgtaccactg
     7861 gctgctgcag tattttttat attaatatca tttcgtttga gggttcatcc tgaagttatt
     7921 tacagcagca gtataaggac aaagatagca ttagctctca tagttattgc taccgttcca
     7981 actataccta ttatagtcat taccagtaat attttaaatt caactattaa tcagtttttt
     8041 actgaaaaaa ccatttctgc gctggaaaaa tctgttgacc ttgcaaatca aatggttctt
     8101 gaagcagaag aaaatctggt gcagcagttg aatcaattga aatatgattt aaacaatcat
     8161 tatgtaagta cagaatatct attacatcct gctttcagcc agcgatatat gtatgcaaaa
     8221 acatcgttga ttgtgacact tgttgaaaca aaaggtgtac tgcaaaataa tgttacattg
     8281 ctgcatgctg ggaacaataa tccagatatt gtatctaata ttgttacctt tctgcaactt
     8341 gcacatattg ctaatattac tcattgcagc agattatcta tatttaataa catctactct
     8401 gttgcttata cacaatatgg tccgtactgt attgtaatgt acagattaat tccaaaagat
     8461 atgtttgcaa ctgctgattt tttaaatacc gcatacagtg aatataagaa gcaggaattt
     8521 cttaaaccgt attttcagac tggtattggc atagcgttaa ttttactttc actgggtatt
     8581 attttacttg ctatcatcct gagcatcatg atatcgcaaa gtataacacg gccagtgctt
     8641 gaactggttg aagcttctga aaagattgca aaaggtgatt tccagataaa tctttccaga
     8701 aaagaacagg atgagatagc tgctttatat gaatcattta accagatggc caaacagctt
     8761 gatcatggca gaaaaatgat gtatcagaca cagaagttac aggcgtggag tgatatagca
     8821 aggaaattaa tacatgagat aaaaaaccct ttaactccta ttcgtctgtc ggcagaaagg
     8881 ctttaccgaa ggtatgagga aaatcaccct gaatttccaa tcattgtaaa agatgcaacc
     8941 ggcactatta ttgaagaagt aaatattttg atgaatatgc tttctgaatt ttcaaaattt
     9001 gccaggttgc ctgaaattaa tctggccgaa gagaatatta atactattct tgaaaattgt
     9061 attgatatat atggaaacga tgcgatttca tttgttacta atcttgatac taatcttcca
     9121 cctgttatgt gtgataaagc attagtgcgc caggcttttt taaatattat tcagaattca
     9181 attgaagcta aaacaacaga aattactatc acaagtatat atgataaaga aaaacaagta
     9241 atatatattc tgtttaaaga taatggtgtg ggcattcctg aagaaaatct atctaaagtt
     9301 tttgaaccaa cattttcaac caaagaaagc ggtatgggtc ttgggctggc tattgttgaa
     9361 aaaattatta ttgaccataa atggaatata acttgtgatt caaaaggaga tggtacacaa
     9421 tttacgatta ctataccttt acaataagat gctgttcaat taagaagctc ttgtattgtt
     9481 atgtgagatt attctattta tgaggtatta tggcaaagat acttgttgtt gatgatgaaa
     9541 aaaacatttt aaataccatg aaagccattt tgcaggatga aggccatact gtatatactg
     9601 ttgaaaatgg caatgatgcg gtacaattta ttaaatcaaa tgagtgtgat gttgtattct
     9661 tagatgtatg gcttcctgat attgatggat tggaagtact gcaacgtatt aagcaaataa
     9721 agcaggatgt ggctgttatc atgatttcgg ggcacgggtc aattgatata gcagttaagt
     9781 caacacggtt tggtgcctac gattttctgg aaaaaccacc ttcaatggag cgggtgataa
     9841 catcattaaa gaatgcgctg gaacagattg aattaaaaag agagaatatt ctattaaaga
     9901 aaaatattac gttagaagat gaaatgattg gcaactcacc tccaatgcag gaggttaagc
     9961 ggattattga tacagctgca tcaaccaatg caagagtttt tataaccggt gaaaatggaa
    10021 caggcaagga actggttgca cgagctattt atcgaaaatc taaacgcagc gataagcctt
    10081 ttgttaaagt caactgtgca gcaatacccg atgagcttat tgaaagtgag ttatttggac
    10141 atgaaaaagg ctcgtttaca ggagctgtcg cccgccgctt gggaaaattt gaattagcag
    10201 acaaaggaac aatattcctt gatgagatat gtgatatgag tcccagcgca caggccaagg
    10261 tgctgcgtgt attgcaggaa cagcagtttg agcgtgtggg tggcaatgaa gtcattacag
    10321 ttgatgtgcg tgttattgct gcaaccaatg tggatgtaaa agaagccatt gaacagggac
    10381 gcttccgtga agacctttac tatcgcctca atgttatccc catttatgta ccgcctcttt
    10441 cagaacgtcg tgaagatata ccattactgg ttgattactt tctggaaaaa tttgcgcgcg
    10501 agcatggcct tggtgttaaa caaatgaacg acagtgccat ggaatttctg gttaattatt
    10561 cgtggccagg taatgtcagg gaacttaaga atgtcattga acggcttacc attatggtgc
    10621 catcagaagt tatacaggta caggatgtta ccaaacatat tgagtcctat gattatgagg
    10681 atactattgc caaagaaaca tccagtttga aaaaagcacg tgaacagttt gaaaaagatt
    10741 atattatcaa agcattgaaa cagtatgata aaaatatttc agccactgcc aaagcgttgg
    10801 gaattgagcg caccaatttg cacagaaaga tccggcaata tcatattaat attgacaggt
    10861 tatagcacgc cagtgtgtat tttacatcag tcaaaactta actacaggca ctattatgta
    10921 ccatttactt aaagaagcaa ttgtgacaca tatacagtat ggcaggcgaa caccactggt
    10981 actggcctat actgatgaaa tcagttattt ttctaacaga aaaaatgttg ctgcacctgt
    11041 tgaattgaat acattccagg aaaaagtgca gcatgcggtg gcacaatgca ataaatgcaa
    11101 aactacagaa aaaaatataa tccatggtgg tgatgcttca tcaggaatca tgataatatt
    11161 aaacccgcca tcgatgcttt catctgttga aaagaaggtg ttgaagcctg atgttgatac
    11221 gatgcttgat aagatgatga aagctattgg tgtccatgtt aaagcctgct atgttacaca
    11281 catgattaaa tgtgaaagcc agcgtgaact acccggtacc atgttttcac attgccagaa
    11341 tcttctggca aaggaaatag aacttgtaaa accacagata attattgtta tgggtgaaat
    11401 gcgtccctta cagaagattg ttaaaaacag tagcggtatc cagtggttta atattgagca
    11461 ccctataact cttttaaaaa accctgaatt aaaaaaacca gcctggaata cattgaagct
    11521 tgttaaaact gtgctggatg gaaaatagat atgtttgtag atgttgctgt aggattccct
    11581 gttgatggat tttttacata cagcactgat gatactagca taacaaaagg gatgcgggtt
    11641 gttgtcaatt tcaacaaccg taaaacaact ggttatgttg ttcatgtcca tgacgacccc
    11701 ccaaattttg acgtgaagcc tgtcataaag gtacttgata cacagcctat ctttgatgat
    11761 cgcctactta ggttagcaca gtttgtttca aatcactatg tgtgttattt tggtgaggca
    11821 ctgggtactg cgttaccttc gggaaaatcg tataacatac gcacaaagcc atttactttt
    11881 ggcgatagct ctaaagaggt aatcctcact gaagagcagg aacaaatata tagggctatt
    11941 atgaaccagc cacagaaggt gcattgcatt tatggtataa ccggcagcgg gaagaccgag
    12001 gtgtacattg ctctggcaaa gaagatgata cagcagggaa aagctgtttt gtatttggtg
    12061 cctgagattt ctatttcctc acagatgttt gagcgcctta aagctgtgtt tggtcagaca
    12121 ttggtgatgt accatagcgg aatgaatcca aaccagcggc ttgtaagctg gaagcgcttt
    12181 tatactggcc aggcaatgat tgctgttggc acacgctctg caatctttat gcaggcccct
    12241 aatttaggcc ttattattat agatgaggag catgacagtt catataaaga aaacagcaca
    12301 ccgcgctacc atgcacgcac ggttgcctgg tacaggcacc ggcaggaagg tgcaatgctg
    12361 gtattgggtt cagcaacacc gtcacttgaa acactgtatg caatacagcg taccagtatt
    12421 gtgcagcacc agttgaacaa acgttttgga actgcaaagc ttcctgaact ggagatagta
    12481 actgtaaagg catcaaatgc acgcactata ttttcaaata cattgctgtt ttcaattaaa
    12541 aaggcaattg aggctagaga gcaggtcata ctgcttttaa acagacgagg ttttgctccg
    12601 gtgatgttgt gtcaggattg tggtacagct gtgcagtgcc ctcattgcag tattagtctt
    12661 acacatcata aacaaaaatt gctatgccac tactgccact atgaagctgt tgtgccagaa
    12721 cactgtagta attgcggttc aaaacatctg gttatggtgg gcagtggtac acagcgtgtt
    12781 gaggaagttt tgcaaaaaat gtttcctgca gcaacggtag tgcggcttga ccaggattca
    12841 gcacgcagaa aaaatttcct tcctgctgtt gtcaatgata tgatgaatgg caacattgac
    12901 attcttttag gaacacagat ggtggcaaag ggatttgact ttcccaatgt atcgctggtt
    12961 ggtgttatgt tagcagatat tgggctttta cttcctgatt tcagggcagt agagcgcacc
    13021 tttgcattgt tagtacaggt agcaggcaga tcagggcgag gtgaggttgg tgggaaggtg
    13081 attgtgcaaa caatcaatcc ggataatccc atatttcatt ttttaaaaaa tcacgattat
    13141 atgggcttct gccagaaaga gctatcaata cgaaaagcat tgaactatcc accctatatt
    13201 cgcctggcgc gtcttgtggt cagaggtgca aaggaggaca ttgtggcaac cgttgccggt
    13261 cagtgcaggg atatattaca ggaactatcg ccacaatttc ctgacataac aatacttggg
    13321 ccctcccagg caccattgca taaaatagca tcacagtacc ggtatcatat tgtgttaaaa
    13381 tcaaacaatg ttgaaaagct aagtggcata ctggcgcagt gtaaaaagaa ggtcaacctg
    13441 cagggaacat atgtagagat agatattgac ccattggatc ttttgtgaaa agcagtggtg
    13501 ttacaacatt acaccgtctt ctgtggtgct atcagattat gataattttt cttccagtac
    13561 aattttcttt cctgcaatag tcagataaaa aatatcatca gtttcttcaa ggcggtcaag
    13621 aatagtaaat gatgcggagc gtttgaattt tgcaagcatg gtgttattgc gtacatagca
    13681 atacagctct tttcccttgc gaacatgaag ggtagaaaca tcaagctcct cttcctgtcc
    13741 tgtgcttaac gtcagaatta ccgtttcaaa atcagcaaag cctttgaaac gaacgcctgt
    13801 cacaaatatt ggggcatcct caaccacaat ggggtatgtg aattgtgcat agtgaatcac
    13861 atatgtccca tcttttgtta tgttgattga tttattaaaa aaatctataa tgcgttgatt
    13921 ggtgaaaggt tgcccattgt gaaaccagtt gccttcagca tcaagcctga tttcgtcaat
    13981 cttttcaccc tgcgctaaaa gctttttaat ttcatcagga atttcaggga attgcatgga
    14041 tgctacacct ttattctttt tcaaagagag tgttttttcg tatgcttcca ttggatgaac
    14101 ggcgctgtcc caaaagtcgt tttgatacaa atttcgtaac gctcattgag taattttgcc
    14161 atattttgtg gcaaaattgt atcgaaatga gcgagtattt cgatataatg tttgcaaaat
    14221 ttactcaacg acctctttta ggaaagccaa aaaaattatt tcagagaaag ttccttttc
//
LOCUS       DAORYL010000076        14038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_3437_length_14038_cov_16.368662, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000076 DAORYL010000000
VERSION     DAORYL010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14038
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3437_length_14038_cov_16.368662"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1027)
                     /locus_tag="PLV81_12125"
     CDS             complement(2..1027)
                     /locus_tag="PLV81_12125"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOM10889.1"
                     /translation="MDNNILFDSSKYFTGWGEVLLRLSPPDTQQLLHTDMFHSYFGGA
                     EANCAVLLSQLGIKTQLLTVLPENELGKAAVRYFRSFGVNTDCVMYKNDSRLGLYFYE
                     KGVGLRPPRLIYDRVHSAFASLQKDSINWEHVLHDCGWFHTTGITLSLSHILSEETVK
                     ACDAVKRSNGIISFDVNYRSTLWKDISHAKAVIQEFVKTIDVLIGNEEHIKKLLLKEN
                     ITTYNDMMDYLFKKYPNVKLLLITHRYTTNASETEIGALASTRQSATEFKVRKLNHVV
                     DRVGSGDAMAAAFIYSFIKGNSLDYCVDFALAAGALAHALDGDNFIVKESDIVAVMEN
                     KSWQLIR"
     gene            complement(1031..2347)
                     /locus_tag="PLV81_12130"
     CDS             complement(1031..2347)
                     /locus_tag="PLV81_12130"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOM10890.1"
                     /translation="MIPQPPKGFNLLKVLGPGLVWSASAIGVSELVFATRSGALFGYL
                     LLWAPIVSLLMKYAIFELVGRYTIATGENVLIAFAQVEIDCKLFKLKTGWVITLFWIV
                     FIASVSGMAGIALSVGSCIYGLVPAISMEMATVCSIVTVGIILYVGSYITLEKISRAL
                     VFIMIVFLVYALIETAPDPVAMADGLIPRAPFHTLRELVPLLGWTGAGAIGVVWYSLW
                     IEGSKRGKADRSIETDEDKSRIKKWIKINQIDLSVNTVITALLTVLFLIAGAVILYPK
                     GIVPKGEQLGLQISHIAGESFGKIGEIIFLVGIFGTLFSTLLANIDGLCRVASNSVWY
                     QRKKDDDVKLRYYRLFVFIYVVFTALFAVVFPAPVILLQVSAIIDTVFLPLVIGLGIY
                     ICHKDLPYIYRPGKTLTITAIASALFFTFFIGLFIWAVIMKVNFSM"
     gene            complement(2374..3075)
                     /locus_tag="PLV81_12135"
     CDS             complement(2374..3075)
                     /locus_tag="PLV81_12135"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOM10891.1"
                     /translation="MSIAFTLIGQSTVCIDIDGLVFITDPWWGSFEFLRAVPLPMDPE
                     KIEPIHYMLVSHNHIDHWCDRAIRLAQARDCEVIGSISAVKRAHKKGVKKINALTPGN
                     SVNCNGIMVTAVPAFHPFAKDAIGFIIQKGSVTIYFSGDTRYHELLQKALTPYSVTIA
                     MIQVACSTYPIVGKDGMDMEAATRFVDEVKPQIVIPIHYQIKGKYLTKQDLENWSIRA
                     KKIILLNGVKTLIPL"
     gene            complement(3122..4081)
                     /locus_tag="PLV81_12140"
     CDS             complement(3122..4081)
                     /locus_tag="PLV81_12140"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOM10892.1"
                     /translation="MKTVVVTGCSGYVGQHVVQRLVDENYAVTGIDIKDFPGKGLPNV
                     TFFKGDIVDRDFVVQSCSGADAVVHCAAALAQFVKDTDRMYAINVTGTRNVIEACQKH
                     TIQQLVFVSSVEVYGIDVPVPCPEDVPLCPVCPYGEHKVIGEQMCIGLAEKGHGVAIL
                     RPPTINGPGQNEPFLVEQMKAVREGKPVILPGGGKTRLQMVDVRDVAQAIVLSLHKIT
                     KGVLVANISSDNVPTIRQMVTALFEHAGKKEKILSVPAPVARGIVNILSFFGVSPLEP
                     QHLEIALRDYVFDNSRAKKALGWYPTKNDIESAIDAYEWFINN"
     gene            complement(4096..4800)
                     /locus_tag="PLV81_12145"
     CDS             complement(4096..4800)
                     /locus_tag="PLV81_12145"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HOM10893.1"
                     /translation="MAPVYSYEETVELCKRLEKTYTPAVCDTLDEMGFMHQAMCSGFT
                     PIMPKAAIAGPAFTMEEAKTRKSTRLKEYDPEFVAKVLEDLFGSMQKGQVVAVNANDF
                     YGAGAWGELMSTTCKYFGGVKGAVVDGPIRDINRILEIEFPVWARGNIPTDSIGRVDL
                     VGMGAPIWCGGVRVNPGDIIFADCDGVVVIPVKDVDLKEVVEKAEEVVQAERRSRQEI
                     RQGMSLVDVYHKYGKL"
     gene            complement(4813..7665)
                     /locus_tag="PLV81_12150"
     CDS             complement(4813..7665)
                     /locus_tag="PLV81_12150"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOM10894.1"
                     /translation="MYGNIVCAALFVLISFSYALATTFNATIYPTPLVYNHKEFYCQR
                     IIIQADQEFEGQIIASIDGVVTKKAVDSKVKEITIDCYRKTITEPSRLVVTIKNNNAI
                     ITKQTLTIPPVKICKIYCVPFTHVDIGFTQSQKNVRAQNISNIQKELLLLEQTKHYPH
                     YARFKLFTEVSWPVIEFLNSAEIPQQEKSKLIAALHKKDFEVGAFVISHQNRFMSPYA
                     LLASLHHTLRIAQKYSINVKTACIHDVMDFSKITRVLYANNIPYCLIGPNDSRYSVPP
                     LSYLTSPDTSARVLVWHTVGLNGYGENFDLNMRLSLPFKDKEFVSMQNSIAYHLAQLE
                     KGYPTEEIQRYYDYDHKHWDYPYDAYLLPYYPAQGGDNQPQNIVPSEIAKTWNNKFIN
                     PKIIIATPHEFFDYISQKYANNIPVLKGEMPPFWGEQIYLDFIQVDPERLIINNWFDR
                     ALYARGKDLVDALLQNSTVDVNSYNLQSLEGYMAIILNNDHNPRPVPFGKTHYTKQDV
                     KDWMNTRNEWVYAPLHSIKDFKLLQENKENKQWRQIHYDANKPIIIENKFYRISFDAD
                     KGCIKSILDKQSQKEWVSKNHMYGFNQYVIGIRGENAARRNYFETIAGFKKVTTTLYN
                     NGNDYRIVVEGSEHAYYRGMDILSEFFNNAFGIHVPPVILKIAYFFYQLFTPSLSITQ
                     EIIIPAGEKRIDFIQHVKGKAPQIAEHYFAYPVNSREMLYDSAYTVLRWGSMDTGGNL
                     IPAAKNIAPFKSINDTLYPFQWMHGLPSSVYFDSFVLHKEDLQYAVFVSQDSKAIVPV
                     KQDDMAGFYHCCIGWTLWGKLGLGRDLPDTIAFKSTFTSFTAQTYNEAIATAYRFSFD
                     ESGFIQSPCIQTNNTHVAIIYNQPLAQNKIVIGMYELLGKKQQAACIINTKRAIQKAY
                     CSDVTGKYIKPVAVKNYSIEITLNPHELSFITLELL"
     gene            complement(7686..8387)
                     /locus_tag="PLV81_12155"
     CDS             complement(7686..8387)
                     /locus_tag="PLV81_12155"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HOM10895.1"
                     /translation="MDTIQPVKRNKLTESIVNVLLTNIKNGTLPPGSKLPPERELMHK
                     LGVGRSTLREAVQSLALMGILDVKPGEGTFVRAISKEEIIGPHIFVPIIDAESMADFF
                     EARLLIEPHIAALASKRKNAQDIDAIEETLVQTKRNIGKGDEVDRWAGQFHLQVARAS
                     KNIVCVRFLEAILSFLVGRRENAEHNKDFLQWEYESHEKIFRAIKQGDESKAYKTMEQ
                     HIQAVLQWYKKLDVV"
     gene            complement(8395..9393)
                     /locus_tag="PLV81_12160"
     CDS             complement(8395..9393)
                     /locus_tag="PLV81_12160"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HOM10896.1"
                     /translation="MIKIAELLPPRPSSLWKMVKQCGVEYVVGGMQLYSGWESMPKDF
                     WPWSYNSLVHVKTTYEDAGFKLEVIESRPPMEKIKLGLPGRDEEIENVIMLIQNMGKL
                     GIPVWCYEWMPVFGWTRTSTTTPVRGGALATSYDHSFMEKAPLTEYGIITEEMLWKNL
                     DYFLKAVVPEAEKAGVKLAMHPDDPPISPIRGVARIMRSVEHFQKLLDMYPSKVNGIA
                     LCQGNFALMTDDLPAVIRTFGKQGKIFFVHFRNVQGTPYKFTETFHDDGMIDMYECMK
                     AYHEVGFDGTMRPDHVPTMEGDSNDNPAYSSIGRLFALGYMKGLLEAVEKSAKDKR"
     gene            10073..12220
                     /gene="glgX"
                     /locus_tag="PLV81_12165"
     CDS             10073..12220
                     /gene="glgX"
                     /locus_tag="PLV81_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HOM10897.1"
                     /translation="MQTVKHHHFVTLPGAPLPLGVHLDRHGARFALFSRHATAVSILL
                     FASSETEEYIEIELDPKLNKTGDIWHIWIAGIREGQLYGYKVDGPYNPLEGHRFNKNK
                     LLLDPYAKAISGNHIWDFSKACGYIKGHPDEDLSFSTDKNYKESPKCIVVDTEFDWYD
                     RPIQTPMEDTIIYELHVKGFTFHPSSQVTHRGTYKGLSEKIPYLKQLGITAVELMPIQ
                     EFDEFENININPLTGERLKNYWGYSTIAFFAPKSTYSSSGTMGQQINEFKEMIRDFHE
                     AGIEVFLDVVFNHTAEGDHLGPTIHFKGLDNSIYYMLKENKRFYQNFSGCGNTFNCNH
                     PLVRDFILDCLRYWVVDMHVDGFRFDLASILGRDQDGNILQNPPLIEKISEDPILRNT
                     KIIAEAWDAGGAYLIGHFPGRWAEWNGKFRDDVRRFWRGDNDTVFNFMQRIIGSPDIY
                     QRTNRSPLHSINFITCHDGFTMLDLVSFNEKHNLANGENNQDGENNNLSCNYGFEGLD
                     APPDIINIRRQQIKNFFTTLLLSQGVPMILAGDEMGRTQQGNNNAYCQDNEISWVNWD
                     RLQQFDDIRRFVELLIQFRKMHPVLRQKRFLSGTIEHGFTNPDCVWHGVNCHNPDLGY
                     YSHSIALHLNGAYAKIPSGLPDNDIYIIYNASEYDLSFSLPKPFNGTHWVRVVDTSLQ
                     SPHDIILPGAPLSSQETYFSKKMSAIVLISAQS"
     gene            12366..14036
                     /locus_tag="PLV81_12170"
     CDS             12366..14036
                     /locus_tag="PLV81_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10898.1"
                     /translation="MIPLLITSLIVVIVILLWAITGNDTKPDTTQPAPPEKMYRRRAS
                     DLEIEEQYPDASKPKRRATDIPLEKTFIDEDFKLPYSAEEIIPPYSRFRIYMRILMNA
                     EVYAKKGDFTTAISLYEGVNERILDAETNTKIEANIDYLKKYKQKQEAQHRQKEAETR
                     SKPNEIKISLDSPIKLPDKIQIGFTPQNFDVDKIVDEITRKIQEKQKLENLEDERINT
                     YRDEISKLTSHLEELKKEVASSLKGITDKEESSEIQKLQSQLNDLTDRMFKLYEEKEK
                     TRAELENLKEVHSKLLESGKQPEVSTVKYEGKLPIQLDPEPILKILEKLPEITAPKPK
                     PVDKDKVKGDIARDILKKLYGDKDKPDELKQTSEIVEKTERQKEEEDWELISDIVKDE
                     KKSELDEVSDEDIFQKILEEDKRKKQDEIEILGDYKKSDEVEYDTDDPETRRKLEEDR
                     KFYEKFLQHDRRKKKELPILKVTYDFKKLPDEFSLSREKNILEYAFYKYRPLLQKADD
                     LIKKRKVRDALNYYRVVMQQNIPPEFKTMIRKNINDLNEYLEKYLTSD"
BASE COUNT         4361 a         3002 c         2475 g         4200 t
ORIGIN      
        1 attacctgat taattgccag cttttatttt ccataactgc gacaatatca ctttccttga
       61 caataaaatt atctccatca agagcatgtg ctaaagctcc tgctgccagg gcaaaatcca
      121 cacagtaatc cagggaatta cctttaataa aagaatagat aaaagctgca gccattgcat
      181 cacctgaacc tacacggtca acgacatgat tcaattttct aactttaaat tctgtagcag
      241 attgccgtgt tgacgccaaa gcgcctattt cagtttcaga tgcgttagtt gtatatctat
      301 gtgttatcag taaaagcttt acatttgggt actttttgaa aagatagtcc atcatatcgt
      361 tataggttgt gatattctcc ttcaatagaa gttttttaat atgttcttca ttcccaatta
      421 acacatctat tgttttgaca aactcctgta ttaccgcttt agcatggctt atatccttcc
      481 ataatgttga acgatagtta acatcaaatg atataatccc attacttcgt tttacagcat
      541 cacacgcttt tactgtttct tcagatagta tgtgcgatag tgaaagcgtt attcctgtag
      601 tatggaacca tccgcaatca tgcaacacat gctcccagtt gatagaatct ttttgcaatg
      661 acgcgaaagc actgtgcact ctatcatata ttaatcgtgg tggccgtaaa ccaacacctt
      721 tttcataaaa atacaggccc aatcgtgaat cgtttttata cattacacaa tccgtattga
      781 cgccaaaact tctgaaatag cgtactgctg cttttcctaa ttcattctca ggcaacaccg
      841 tcaataattg tgtctttatt cccaattgcg atagtaacac tgcgcagtta gcttcagcac
      901 caccaaaata tgaatgaaac atatctgtat gcaataattg ctgagtatca ggtggtgata
      961 atcgtaaaag cacctcaccc cagccggtaa aatattttga gctatcgaat aaaatgttat
     1021 tatccattgt ttacattgaa aaattaactt tcattatcac agcccagata aatagtccaa
     1081 taaaaaatgt aaaaaaaagt gctgatgcta ttgcagttat ggttaatgtt ttcccaggcc
     1141 tgtatatata cggcaaatcc ttatggcaaa tatatatgcc taaaccaata acaagtggaa
     1201 gaaaaactgt atctataatt gcagaaacct gcaataagat aacaggcgca gggaaaacca
     1261 ccgcaaacag tgctgtaaac acaacgtaaa taaagacaaa caatcgataa taacgtaact
     1321 tcacatcgtc atccttcttg cgctggtacc acactgaatt ggaagctacg cggcataatc
     1381 catcgatatt tgcaagcaac gtgctgaaca gtgttccaaa tatcccaaca aggaaaatta
     1441 tttcacctat tttgccaaag ctctcccctg caatgtgtga tatctgcaaa cccaattgct
     1501 ctcctttagg aactattcct tttggatata atatgaccgc tccagcaatt agaaataata
     1561 ctgttaaaag cgcagtaatg acagtattta cggataaatc tatctgatta atttttatcc
     1621 attttttaat cctgctttta tcttcatcgg tttctattga tctatcagcc tttcctcttt
     1681 tactaccctc gatccacaac gaataccaca caactccaat agcaccagca cctgtccacc
     1741 ctaaaagtgg aaccagttcg cgcaatgtat ggaatggagc ccgtggtata agtccatctg
     1801 ccatcgctac aggatctggt gccgtttcta tcaatgcata caccagaaac actatcataa
     1861 taaaaacaag cgcccgggat attttttcta aagttatgta gcttcctaca tagagtatta
     1921 ttccaacagt tactatcgaa cacaccgttg ccatttccat ggaaattgct ggcaccagtc
     1981 catatatgca gcttccaact gataatgcta ttcctgccat ccccgaaaca cttgcaataa
     2041 atactatcca gaaaagagta ataacccacc ccgttttcag tttgaatagt ttgcaatcta
     2101 tctcaacctg tgcaaaagca attaagacat tttcaccggt tgcgatggtg taacgtccaa
     2161 caagctcaaa gattgcatat ttcatcaaaa gagaaacaat tggtgcccac agcagtaaat
     2221 atccaaacag tgcaccagaa cgagtggcaa acacaagctc tgacacccca atggcacttg
     2281 ccgaccatac cagaccaggc ccaaggactt tcaacaggtt aaaccctttt ggtggctgtg
     2341 gtatcatacc tccccccttt ttttttagta tatttaaagt ggtatcaatg ttttaactcc
     2401 atttagaagt ataatttttt ttgcccgtat actccagttt tctaaatcct gttttgttag
     2461 atatttcccc ttaatctgat agtgaatggg tataacaatc tgcggcttaa cctcatcaac
     2521 aaaacgagta gcagcttcca tatccatgcc atctttgcct acaataggat aggtggagca
     2581 ggcaacctgt atcatggcga tagttactga gtaaggggtc agagcctttt gtaaaagctc
     2641 atggtaccgc gtatcacccg aaaaatatat ggtaactgaa cccttttgta taataaatcc
     2701 tatggcatcc tttgcaaaag gatgaaatgc aggcactgct gttaccatga taccattaca
     2761 attaacacta tttcctgggg tcagagcatt aatttttttt actcctttct tgtgtgcccg
     2821 tttgacagca ctgattgatc caataacctc acaatctctt gcctgtgcca acctgattgc
     2881 tctatcacac cagtgatcaa tatgattatg tgaaacaagc atataatgaa tgggttcaat
     2941 tttttcagga tccatcggta gcggaacagc ccgcaagaat tcaaatgatc cccaccaggg
     3001 atcggttatg aagactaatc catcaatatc tatacatact gtactttgcc caattaatgt
     3061 aaatgcaatg ctcataggca aacctcctca gaacatgcgt taatatattt tattatctcc
     3121 ctcaattatt tataaaccat tcgtatgcat caatcgcact ttctatatca tttttggttg
     3181 gataccagcc taatgctttt tttgctctgc tattatcaaa cacatagtcg cgcaatgcta
     3241 tctcaaggtg ctgtggttca agtggcgaca caccaaaaaa tgaaagaatg ttaacaatac
     3301 cccgtgcaac tggtgcggga accgaaagga ttttttcctt ttttcctgca tgctcaaata
     3361 atgctgttac catctggcga atggttggaa cattatcgct tgaaatattt gcaacaagca
     3421 ccccttttgt tattttatgt agcgatagca caatggcctg tgcaacatcc cgtacatcta
     3481 ccatctgcaa tcgtgttttt cctccaccag ggaggataac aggtttacct tctcttactg
     3541 ccttcatctg ctcaacaagg aagggttcat tttgtcccgg gccattaatt gttggtggcc
     3601 ttaaaatggc aacaccatgc cctttctcag ccaggccaat acacatttgc tcccctataa
     3661 ccttatgttc accataggga catacaggac acagtggtac atcctcagga cagggtactg
     3721 gcacatcaat accatacacc tctacagaag atacaaacac cagttgttgt atagtgtgtt
     3781 tttgacatgc ttcaatgaca tttctggtac cggtgacatt tattgcatac attctatcag
     3841 tatctttaac aaattgagca agcgctgcag cacaatgtac caccgcatcc gcaccactgc
     3901 atgattgtac cacaaaatct ctatcaacaa tatcaccctt gaaaaatgta acattgggta
     3961 agcccttgcc aggaaaatcc tttatatcaa tgccagtaac tgcatagttt tcatcaacaa
     4021 gcctttgtac aacatgttgc ccaacatatc ctgaacaccc cgtaacaact actgttttca
     4081 tgtcaataac cctcttcaca gcttcccata cttatggtac acatcaacca gtgacatgcc
     4141 ctgccgtatt tcctgtcgtg aacggcgttc agcctgaaca acctcttcag ctttctcaac
     4201 cacctctttt aaatctacat cctttacggg gatgaccaca acgccatcac agtcagcaaa
     4261 aataatatca cctggattta cccgcacacc accacaccaa attggcgcac ccataccaac
     4321 cagatcaacc ctgccaatag agtcagtagg gatgttgcct cgtgcccaga caggaaattc
     4381 aatttccaga atgcgattaa tatcacgaat tggaccatcc accacggcac cctttacgcc
     4441 accaaagtat ttacaggttg ttgacattag ctcaccccac gcacctgcac cataaaagtc
     4501 atttgcatta accgccacaa cctgtccctt ttgcatgctt ccaaataagt cctccagcac
     4561 ttttgcaaca aattctggat catattcttt tagccgtgtg cttttgcgtg tttttgcttc
     4621 ttccatggta aacgcaggcc ctgcaatagc cgcttttggc attatgggcg taaaccctga
     4681 gcacattgcc tgatgcataa accccatttc atcaagtgtg tcacaaaccg ctggcgtgta
     4741 cgtcttttcc aaccttttgc ataactcaac ggtttcttca tatgaatata ctggtgccat
     4801 acattacctc cattataata attctaatgt tataaatgat aactcatgag gattcagagt
     4861 tatttctatg ctatagtttt ttactgcaac aggtttgata tatttgccgg taacatctga
     4921 acaatacgct ttttgtattg ctctctttgt attaatgata catgcagcct gttgcttctt
     4981 gcccaatagc tcatacatac caatgactat cttgttttgt gcaaggggtt gattgtagat
     5041 tatagctaca tgtgtattgt ttgtttgtat acatggtgat tgaataaaac ctgattcatc
     5101 aaaactaaac ctgtacgcag ttgctatcgc ttcattgtat gtttgtgcag taaaacttgt
     5161 gaatgtactt ttaaaggcga tagtatctgg caaatctcta cccaacccca atttccccca
     5221 caatgtccat ccaatacagc agtggtaaaa gcctgccata tcatcctgtt tgacaggaac
     5281 tatcgctttg ctgtcctgtg acacaaatac tgcatactgc aaatcttctt tatgtaaaac
     5341 aaacgagtca aaatataccg atgaaggcaa accatgcatc cactggaaag gatacaacgt
     5401 atcgtttata cttttaaatg gcgctatgtt ctttgcagca ggtataagat tgccgccagt
     5461 atccattgat ccccatcgca atactgtata tgcagaatca tatagcattt cccgggagtt
     5521 tacaggataa gcaaagtagt gttcagcgat ctgaggagct ttacctttaa catgctggat
     5581 aaaatctata cgcttttcgc ctgcagggat gataatttcc tgagtaatcg acagtgacgg
     5641 tgtaaaaagt tgatagaaaa agtatgctat cttcaatatg acaggtggca catgtatgcc
     5701 aaaggcattg ttaaaaaatt cagaaagtat gtccatgccc ctgtaatacg catgctctga
     5761 cccttctaca actatgcgat aatcattacc attattatac agggtggtag taaccttttt
     5821 gaaaccagca attgtttcaa agtaattgcg cctggcagca ttttccccac gaatgccaat
     5881 aacatactgg ttgaatccgt acatatgatt tttactaacc cactccttct gcgattgctt
     5941 atcaagtatg cttttgatac atcctttgtc tgcatcaaat gatatcctgt aaaatttatt
     6001 ttctataatt attggtttat ttgcatcgta gtgaatctgt ctccactgtt tgttttcttt
     6061 attctcctgc aaaagcttga aatctttgat actgtgcagg ggggcatata cccattcatt
     6121 gcgtgtattc atccagtctt taacatcctg ttttgtataa tgggtttttc caaaaggcac
     6181 cggacgtgga ttgtgatcat tattgagtat aattgccata tacccttcaa gactctgcag
     6241 attatagcta tttacatcaa ccgtactgtt ttgcagcaaa gcatcaacaa ggtctttccc
     6301 acgcgcatat agtgctctat caaaccaatt attaataatc aggcgttcgg ggtctacctg
     6361 aataaaatcc aggtagatct gctcacccca gaatggtggc atttcccctt tgagtactgg
     6421 gatattgttt gcatactttt gtgaaatgta atcaaagaac tcgtgaggtg ttgcaatgat
     6481 aatctttgga ttaataaact tgttgttcca tgttttagct atttctgaag ggactatatt
     6541 ttgtggctga ttatcaccac cctgtgcagg ataatatggc aaaaggtatg catcataggg
     6601 ataatcccag tgtttatgat cataatcata gtagcgctgt atttcttctg tggggtagcc
     6661 tttttcaagc tgggctaaat gatatgcaat actgttttgc atgcttacaa attctttatc
     6721 tttaaaaggg agggacagac gcatattcaa atcaaaattt tcaccatagc catttaaccc
     6781 aacagtatgc cacaccagaa cccttgcact tgtatcaggt gaagtaagat aagaaagggg
     6841 cggcacacta taccgtgaat cgttaggccc aatcaaacaa tagggaatat tatttgcata
     6901 caagaccctg gtaatctttg aaaagtccat gacatcatga atacatgcgg tttttacatt
     6961 tattgaatac ttttgtgcaa tgcgcagagt atggtgcaat gatgcaagca gcgcatacgg
     7021 actcatgaat ctattctgat gcgatataac aaatgctccc acttcaaaat cttttttatg
     7081 taaggcagct ataagtttag atttttcctg ctgtggtatt tcagcactat tcagaaattc
     7141 aataacaggc cagctgactt cagtaaaaag cttgaagcgt gcatagtgtg ggtagtgttt
     7201 tgtttgttca aggagcaaaa gctctttctg tatatttgaa atattctgtg cacgcacatt
     7261 tttttgcgat tgcgtaaacc ctatatcgac atgggtaaat ggaacacaat agattttgca
     7321 tatctttacc ggtggaatag ttagcgtttg ttttgttata attgcgttat tattttttat
     7381 agtaacaact aagcgtgaag gttcagtgat agtttttctg taacaatcga tagttatttc
     7441 ttttactttg ctatcaacag ccttttttgt aacaacacca tcaattgatg caattatctg
     7501 gccttcaaat tcctgatcag cctgtatgat tattctctgg caatagaact ctttatggtt
     7561 atatactaac ggcgttggat atatggtggc attaaacgtg gttgccaagg cataactaaa
     7621 agaaattaac acaaacaatg ctgcacacac tatgttccca tacacagcaa acctccataa
     7681 aaatatcata caacatctaa ttttttgtac cactgtaaaa cagcctgtat atgttgttcc
     7741 atagttttat atgctttaga ttcatccccc tgttttattg ccctaaaaat tttttcatgc
     7801 gattcgtatt cccattgcaa aaagtctttg ttatgttcag cattttcgcg cctccccacc
     7861 aaaaatgaaa ggatagcttc aagaaaacga acacatacaa tgttttttga agctctggca
     7921 acctgcaagt gaaactggcc agcccagcgg tcaacttcat cccctttgcc aatattccgt
     7981 tttgtttgta ccagcgtttc ttcaatagca tcaatatcct gtgcattctt tcgtttgctt
     8041 gcaagggctg caatatgggg ttcaattaaa aggcgtgctt caaaaaaatc tgccatactc
     8101 tcagcatcta ttattggaac aaaaatatga ggcccaataa tttcttcttt ggagatagct
     8161 cttacaaatg ttccttcgcc tggttttaca tcaagaatcc ccattaaggc caatgactgt
     8221 acagcctcgc gtaaggttga ccgccctact cctaatttat gcatcaattc acgttcaggt
     8281 ggcaatttgc ttcctggcgg aagcgtgcca ttttttatat tggtcaacaa tacatttact
     8341 attgattcag ttaatttgtt acgctttaca ggttgtatgg tatccatgta catatcaccg
     8401 tttatccttt gcactttttt ccactgcttc aagtaaacct ttcatgtatc ctaaggcaaa
     8461 aagtctgcca attgatgaat acgccgggtt atcgttacta tcgccttcca ttgttggtac
     8521 gtgatcaggc ctcatggttc catcaaatcc aacttcgtgg tatgctttca tgcattcata
     8581 catatcaatc atgccatcat catggaatgt ttcagtaaat ttatatggag ttccctgcac
     8641 atttctaaag tgaacaaaaa agattttccc ctgcttacca aatgttctga tgactgctgg
     8701 aagatcatcc gtcatgagag caaaatttcc ctgacacagt gcaatgccat tgaccttgct
     8761 ggggtacata tctaaaagct tttgaaaatg ttcaaccgaa cgcataatcc gtgcaacacc
     8821 acgaatagga gaaatgggag gatcgtcagg gtgcatggct aattttactc ctgctttttc
     8881 agcctcagga accactgctt ttaaaaaata gtctaaattt ttccacaaca tttcttcagt
     8941 aataatgcca tactcagtca gtggcgcttt ttccataaat gagtgatcat acgatgttgc
     9001 cagcgctcca ccacgaacag gtgtggttgt tgaggttcgt gtccatccaa atacaggcat
     9061 ccactcataa caccacacag gaataccaag tttgcccata ttctgtatca gcattattac
     9121 attttcaatt tcttcatccc tgcctggtaa tcccaatttt attttttcca ttggaggcct
     9181 tgactcaatg acttccagtt taaatccagc atcttcatac gtagttttaa catgcaccaa
     9241 agaattatag ctccatggcc agaaatcttt gggcatactt tcccatcctg aatacagttg
     9301 catgccacct acaacatact caacaccaca ctgttttacc atcttccata gcgaagaagg
     9361 acgaggtggc agaagttcag caattttaat catatactgt ttacttcctt aaaaaattta
     9421 aaatttactt ttgttatcat caatctgtaa cattaacaat catgaatagt acaaatattt
     9481 ctaatccagt tataccaggt ataaatctga atatctgatg gtctgatgtt atgttaatat
     9541 acccaatttg tcaacatttt ttaaaaaaat tatttatgta tttctttttt ttatacaaat
     9601 gattatgtca tatatattta tatatgggta tcaaggagtt aattgtgaat ggacttcatt
     9661 taccctgtat aatctttatt aatgtaaatg tacgtgcaat gaaaatcttt cagggatagg
     9721 tttccaggat acaattatat ttccatgtct tatcatcatg tcccttaatt gcattaaagc
     9781 atccccctgc tttggcctga aaaaaacaaa atcacccgct tcacgttttg ttaaagaaat
     9841 tgtttttagc atactttaaa atttgagctg ctataaatat tgccggcaaa tgatctttaa
     9901 gagtaaagaa atcaaaagta gatggttgaa ccacacagta attcattatc agacagtcaa
     9961 atatattttc cagaatgctt ttgttacaat aataaattta attgacgctt atatgtatca
    10021 tacttagaat aataaattga aaattactaa aagacaatta ttgctaacta ctatgcaaac
    10081 tgtaaaacat catcattttg ttacgttgcc gggagcacca ctcccgttag gtgtacatct
    10141 ggacagacat ggtgcacggt ttgcactttt cagccgacac gctactgctg tatcaatact
    10201 gctttttgct tcttcagaaa ccgaagaata cattgaaatt gaacttgacc caaagctcaa
    10261 taaaaccggc gacatatggc atatatggat tgcaggaata cgggaaggac agttgtacgg
    10321 atataaggtt gatgggcctt ataatcctct ggaaggtcac cgttttaata aaaacaagct
    10381 ccttttagac ccctatgcaa aagccatcag cggcaatcat atatgggatt tcagtaaagc
    10441 atgtggatat ataaaagggc atccggatga agacctttct ttttccactg acaagaacta
    10501 taaggaatca ccaaaatgca ttgttgttga tacagaattt gactggtatg atcgccccat
    10561 tcagactccc atggaagata ctattattta tgaactccat gttaaagggt tcactttcca
    10621 tccttcatca caggttaccc atagaggaac atacaaaggt ctttctgaaa aaatcccata
    10681 tcttaagcaa ttaggtataa ctgctgtaga acttatgccc attcaggaat ttgatgaatt
    10741 tgaaaatatc aatataaacc cattaaccgg tgagcggctg aaaaactact ggggatacag
    10801 caccattgct ttttttgcac ccaaatcaac gtattcatcc agtggcacaa tgggacagca
    10861 gattaatgaa tttaaagaaa tgatccgtga tttccatgaa gcgggtattg aagtgtttct
    10921 tgatgttgtt tttaatcaca ccgccgaggg tgaccacctg ggaccaacta tacattttaa
    10981 gggcttagat aattccattt actacatgct caaggaaaac aagcgttttt accagaactt
    11041 ttcaggatgc ggcaatacgt ttaactgtaa ccacccgctg gtgcgcgact tcatccttga
    11101 ttgcctgcgt tactgggttg tggacatgca tgttgatggc ttcaggtttg accttgcatc
    11161 aatattaggt cgtgaccagg atggcaatat cttacaaaac ccacctttga tagaaaaaat
    11221 atctgaagat cccatattgc gcaataccaa aataattgct gaagcctggg atgcaggcgg
    11281 ggcatactta attggccatt tcccgggaag atgggctgaa tggaatggga aattccgcga
    11341 tgatgtgcgc cgtttctggc ggggtgataa tgataccgtt ttcaatttta tgcagcgaat
    11401 tattggaagc cctgacattt accagcggac aaatcgtagc ccgctgcaca gcattaactt
    11461 cattacctgc catgacggat tcaccatgct tgatttagta tcctttaacg aaaagcataa
    11521 cctggccaat ggtgaaaaca atcaggatgg cgaaaataac aacttaagct gtaattacgg
    11581 ctttgaaggg ttagatgccc caccagacat cataaacatt cgcaggcaac agataaagaa
    11641 tttcttcacc acgctgcttt tatcgcaggg tgttcctatg atacttgccg gcgatgagat
    11701 gggcagaacc caacagggca acaacaacgc atactgtcag gataatgaaa tttcctgggt
    11761 caactgggac agattacaac aatttgatga tatcaggcgt tttgtggagc ttttgattca
    11821 atttcgtaaa atgcatccgg tactcaggca aaagcgcttt ttatcaggta ctatcgaaca
    11881 tgggtttaca aatcctgatt gtgtgtggca tggtgtcaat tgccacaatc ctgacttagg
    11941 gtactacagt cattccattg ccctgcatct gaatggcgca tatgcaaaga ttccatcagg
    12001 attgccagat aatgacatat atatcatata caatgcatct gaatatgatc tttcattttc
    12061 attgccaaaa ccatttaacg gcacccactg ggtacgtgtg gtcgacacct cattgcaatc
    12121 accacatgat attatattgc caggtgcacc tctctcatct caggaaacgt atttcagcaa
    12181 aaaaatgtcg gctattgttt tgatttcggc tcagtcatga cgatagtata ctaaagggct
    12241 tttaccggta actatcgcac atactatttt ttgataatta tattgcattg catgcatgta
    12301 ttgtgtattt taatacatat gggcgatagt tactaataat ttcagaggat accatagtat
    12361 aacgcatgat accattactc atcacatcgc ttattgtcgt catcgtcata ttgctgtggg
    12421 caataacagg caatgacaca aagcctgata caacccagcc tgctccacct gaaaaaatgt
    12481 accgccgccg tgcaagcgac ttagaaatag aagaacaata ccctgatgcc agcaagccaa
    12541 aacgaagggc aaccgatatc cccctggaaa aaaccttcat tgatgaagat tttaaattgc
    12601 catactcagc tgaagagata attccgcctt actcacggtt caggatttac atgcggatac
    12661 tgatgaatgc cgaggtgtat gctaaaaagg gcgattttac cactgctatt tcgttatacg
    12721 agggtgtaaa tgaacgaatc cttgatgcag aaaccaatac aaaaatagaa gctaatattg
    12781 attaccttaa aaaatacaaa caaaagcagg aagcacagca ccggcaaaaa gaagccgaaa
    12841 ccagaagcaa accaaatgaa ataaaaattt cactggatag cccaataaaa ttacccgata
    12901 aaattcagat tggatttacc cctcagaact ttgatgttga caaaatagta gacgaaataa
    12961 cccgaaaaat tcaggaaaaa cagaagttag aaaatcttga agatgaacgc atcaatactt
    13021 acagggatga aatcagcaaa ttaacatctc accttgaaga gctcaagaaa gaagtagcca
    13081 gttcattgaa gggtatcact gataaagaag aatcatctga aattcagaag ttgcaaagtc
    13141 agttaaatga tcttaccgac aggatgttca agctatacga agaaaaagag aaaacacggg
    13201 cagaactgga aaatttaaaa gaagttcatt caaaactttt agaatcaggc aaacagcctg
    13261 aggtttctac tgtcaagtat gagggtaaat tgcccattca acttgaccct gaacccatat
    13321 taaaaattct ggaaaaactg cctgaaatta cagccccaaa acccaaacca gttgataagg
    13381 acaaggttaa aggcgatatt gccagagata tccttaaaaa attatatggc gataaggaca
    13441 aaccggatga attaaaacaa acatctgaaa tagttgaaaa aacggaacgg caaaaagaag
    13501 aagaggactg ggaacttatc agtgacattg taaaagatga gaaaaaaagc gaactggatg
    13561 aagtcagtga cgaggatatt ttccagaaaa tactggaaga agataaacgt aaaaaacagg
    13621 acgaaattga aatattaggt gattataaaa aatccgatga agttgaatat gatacggatg
    13681 acccagaaac acggcgcaaa ctggaagaag accggaaatt ctacgaaaaa tttttacagc
    13741 acgacagacg caagaaaaaa gagctgccaa tattaaaagt aacctatgac tttaagaagc
    13801 ttcctgatga atttagcctt tcacgggaaa agaatatcct ggaatatgcg ttttataaat
    13861 accgcccctt gcttcagaaa gccgatgacc ttataaagaa gcgaaaagtg cgagatgccc
    13921 tcaattatta cagggtagtc atgcaacaga atataccccc tgaatttaaa accatgatac
    13981 gaaaaaatat caatgacctg aatgaatatc ttgaaaaata ccttacctca gattaagt
//
LOCUS       DAORYL010000077        13708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_3530_length_13708_cov_13.237384, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000077 DAORYL010000000
VERSION     DAORYL010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13708
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3530_length_13708_cov_13.237384"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PLV81_12175"
     CDS             <1..598
                     /locus_tag="PLV81_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10899.1"
                     /translation="GGTVNMDEVLYIGPSIAYQFSDKWSTTLVALATPQKYTWEMGTE
                     RMELRRYDVDLALNYQINRYIKVFGGGKYLAFAYHNKTNGKDGIHHALGPGGGISFTM
                     PIITNVYLLANLSGLYIYGKQQEEQTTGDQSINFYEIGANGSIQLAYYIPSVAVTLAA
                     GYRYQYVESHYTENSSANPDLEHTFKGFTVLLVKSFDL"
     gene            722..2098
                     /locus_tag="PLV81_12180"
     CDS             722..2098
                     /locus_tag="PLV81_12180"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HOM10900.1"
                     /translation="MISFEGYDTVIKKVYQSGNEHVFTFWDDLTIEQKKHLLQELSLI
                     NFDEINELYALTKKHIIPMNFEPAPFTRLPQTPEEHKAFEYAKQVGIDTIKKGKVASF
                     VVAGGQGSRLGFDGPKGIFPIGPVSNKTLFQIHAEKIKAYEKKFGIDIPWCIMTSESN
                     HQATVDYFEKHNFFTLNKDKVFIFKQNMIPSLDEQGKLILETPYSIFRNPDGHGGSLT
                     ALYTSGTLNKLLTMGIEVLSYFQVDNPLVRIIDPVFIGFHVKHNAEVSSKALQKAYPE
                     EKIGVFVKFSNGRIGVVEYSDLPQEKQYEKDEQGNLRYGAGSIAIHLFNTHFIEKIAQ
                     GNISLPFHVAKKSIPQYTNNGKVTITGYKFEKFVFDALPLTEHNFILETKREEEFAPV
                     KNKSGVDSVDTAQELMINLYKKWLLQKNIPVPKVVEKIEISPLYAVEPDDLPDSIAIP
                     QQKEVYLA"
     gene            2095..2952
                     /gene="argB"
                     /locus_tag="PLV81_12185"
     CDS             2095..2952
                     /gene="argB"
                     /locus_tag="PLV81_12185"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOM10901.1"
                     /translation="MKRYIEKVNTLIESFPYIKEFYGKTIVIKYGGHAMETDEGKRSF
                     AQDMVLLKYVGINPVIVHGGGPQINTLLKQVGVESSFVGGMRVTDAPTMEIVEMVLVG
                     KVNKEIVNYINLAGGKAVGLSGKDGNLFVAQKIYHDVNGQKLDIGQVGTIQQVNPLII
                     ETLDREKFIPVIAPIGCDENGVTYNINADIAAGKLAEALKAEKLILLTDVEGIKIDDT
                     LVSTLRKEEVDNLIVNNKITGGMIPKVNCCLSALKEGVSKTHIIDGRVEHAVLLEIFT
                     DAGIGTEII"
     gene            complement(3014..4909)
                     /locus_tag="PLV81_12190"
     CDS             complement(3014..4909)
                     /locus_tag="PLV81_12190"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HOM10902.1"
                     /translation="MITIVLPTIQAFAGTIVLDNIHDTYDTSPYISMFEDPTSQLSAQ
                     DIIKNDLLKKFQDNTSKGINKGYSNSSWWFYFSVHNRSSHTHWLVDLSFPTMDYFEFY
                     NVNEKGITCIAKGGDIFPFGHRKIALNNFILPLTLGYNEKAHYLIRVKTTSEVIIPLQ
                     IRTYTSLLENSIKEYGGLWFYFGIMMAMFLYNLFLLITLRDISYLYYILYVLFSALSN
                     ISFNGIAYQFFWPDSPWFHSVSIVFLGNLAIISFMLFTRNFLMTRNFLHWYDTLLKIF
                     IALIVPVIIAIPLAPLSFSIICLNTEVIVATLLAFIACVIAVKNKYKPAYFYLASMIA
                     VFLGVSLISLRNFGILPDVFITRYGFHLGTSIDVILLSIALGYRYNLIKDENEKLVIL
                     KNEIEIARTIYNSIVQTNIPELTNVAIETILMPTEVIGGDIAEIHLINEDTVGIFIAD
                     ISGHGIPAAIGAAMVKMACAHASQYDEFPDKYLLSIENTLSQRIGNQFVTAIYAVVDT
                     KKMTVATSNAGHYAMVLCKVDGSIVQLRPKGGAIGFYPNAFFAVARENIGKGDRIVLF
                     TDGIVELRNTKGELFGEDRFIDIIREYNGKSIKALKNEILAAAYRWIYPHKNFDDDFT
                     LVIVDIV"
     gene            5340..5540
                     /locus_tag="PLV81_12195"
     CDS             5340..5540
                     /locus_tag="PLV81_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOM10903.1"
                     /translation="MQKNMGTIDRLLRTIIAIVIIVLYFTGQITGTAAIILGILAVVF
                     LLTSAVSFCPLYVPLKINTKKQ"
     gene            complement(5627..6226)
                     /locus_tag="PLV81_12200"
     CDS             complement(5627..6226)
                     /locus_tag="PLV81_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010479675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOM10904.1"
                     /translation="MKHDIILPRPLYDGNVSIEYTLKQRRSVRRFSKMAISLQELSQI
                     LWAASGISQGSYRTAPSAGATYPLNCYVAAGNVEELEKGVYRYIVKNHGLKRIAPYDL
                     RSALCRAALHQTFIEEAPIVIVITAIYERTTGHYGQRGIRYVHMEAGHAGQNISLQAV
                     ALGLGSVMVGAFQDEMVKEIVSLGKGEEPLYIIPVGKPA"
     gene            6492..7607
                     /locus_tag="PLV81_12205"
     CDS             6492..7607
                     /locus_tag="PLV81_12205"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HOM10905.1"
                     /translation="MKKIFFVVLLLTITIPLFAKMQYDDERIFTRAEITKIEKHRIAG
                     DHNYPLYETKIHVTILDGVLKGQTKVATFRGEHDLPKDMFYKQGDVVFIGISKTGMES
                     TPEYISLYDIDNTTMILILTVMLILSVIVIGKVKGFFSLMALLITILLLFFVLIPLTL
                     KGYPPLPLAIVISIISTVITLPVIAGFKKKTFAAILGASAGIIIASILAYIVGNIMHL
                     SGFVTNEMLTVFYVADVAIDIRGLALASIIIAALGAVMDVCISIASATAEIFNANPDL
                     SPKDAFRSVLTVGTDILGSMVNTLVLAYVGSSLSLILLISMRIQPGMPVWMIFNYNPI
                     LSEIVKSIIGSIGMFICIPITAYISVHLYGRKDGAAR"
     gene            complement(7651..9555)
                     /locus_tag="PLV81_12210"
     CDS             complement(7651..9555)
                     /locus_tag="PLV81_12210"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOM10906.1"
                     /translation="MIHKWYIQQLTFPEILNRNAIQFGTRRAQWWKTGPNTTTSITYA
                     ELGIIIKQLTAGLMSLGVEKGDRVAIASHTAPQWLWADYSILCAGGITVCVYPTLSAK
                     EMSFIINDSGSKILYVQDEEILLRALSAIQEMPTLEKIIIMNNDYNGNDSRVVTLQQV
                     RDFGIKLLAQDRLAYERRWRSVNILDPMTIVYTSGTTGRQKGAVHTHFSINAACCRDL
                     RIVPEYREDDVLLSFLPLAHTYERECGHGTAMMAAATIAYSSPMTLVEDLQIFRPTVF
                     MSVPRIYERIFMAMRDMAAKSPIKKKIFDFAIKTGLQLTEARADKNGFVDMSEGIDFT
                     EGVGRWLAFKYKLVDKLVFSKVREKLGGRFRFAFSAAGSLPADLCKVFMAMGVRIYEG
                     YGATETCNTVNLNLPHKVLPGYVGPVCNGVEGRVASDGEWQVRGDNIFLNYWNNPEAT
                     KEAFTEDGFYKTGDIVQMGPDGYIKIVDRKKGIMVLDTGKNVPAAKIESLFSVSRYVD
                     IVFPIADDRPFVGALVVPKFDVFIELFDKEGIPYDKSKLVFFGEGAARMCIGVGEDFI
                     QNEKLKKLVDEDIQLANSELEEYERIKKYVIINRKFTEATGEMTPTLKVKRNIIMKNF
                     EKEINELFKR"
     gene            9975..10700
                     /locus_tag="PLV81_12215"
     CDS             9975..10700
                     /locus_tag="PLV81_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10907.1"
                     /translation="MMDAQPIRFVVTSNTSPDSPYEEFNPQLTNTLLSINKENPVFTM
                     HCGNIIHGGSSIMGINVIDVEKQFQQFFKFAKKLNCILFTACGPLDTYNDSTAIYTKF
                     TGKDAWYSFNYGTNHFIVLYSTSKKTLSISDRQLSWLKEDLKKHSHAHIFVFSYFLPD
                     IQRTKQQFANDSHQKLHELLSQYTVKAFFGVTGLTQPVTIGTIQYIPVPCDNSYTNKY
                     KKAIRYYVVDFDGQYITLVPRYF"
     gene            10719..11000
                     /locus_tag="PLV81_12220"
     CDS             10719..11000
                     /locus_tag="PLV81_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10908.1"
                     /translation="MQREIKFDSIHEFINALHASNITKAAFTQINEKRAVEVKPGTLQ
                     VVDVVIAEILAYKDATIYKCKIENADLDDLYTMLEDEGFELTKRSRNIT"
     gene            11003..11962
                     /locus_tag="PLV81_12225"
     CDS             11003..11962
                     /locus_tag="PLV81_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015413676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOM10909.1"
                     /translation="MEAIVTIENLTISFTNLYGKSYAVRNLSLSIPKGKTLGLVGESG
                     CGKSVTAYSILNLVPKPGKIESGTITYNSIDLLHCSENTLRSIRGKNIAMIFQEPMTS
                     LNPVFTIGYQVQEAVELHLQYSKQEAKEYTIELLGKAGIPNAAQRYYAYPHELSGGLR
                     QRAMIAMALAAHPDLLIADEPTTALDVTIQSQILDLLLTLQATEHMSMLLITHDLGIV
                     ANTADYIAIMYAGEIVEYAPTKEIFENPLHPYTHGLMNCIPSLHKTAKRLYTIPGNVP
                     IITQPPQGCVFHPRCSLGDDECLQKPVNLAGRKKDHLVRCIKV"
     gene            complement(12013..12216)
                     /locus_tag="PLV81_12230"
     CDS             complement(12013..12216)
                     /locus_tag="PLV81_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10910.1"
                     /translation="MGNSICSSCINRNICGRESLAHCEHYDVVLTRAEVVDLLKTTRE
                     KDPNKFQTILNGIKKTYHINLAY"
     gene            complement(12532..13395)
                     /locus_tag="PLV81_12235"
     CDS             complement(12532..13395)
                     /locus_tag="PLV81_12235"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOM10911.1"
                     /translation="MHRRRFIWVIYALLCALFLAIGDVLVKNAPKIHPVTVAAGRIFF
                     SLPVLWAIALIKGIPAVDWRIAYVFIFSPPLEVVALVLYMRALQISPMYRTLPFLAFT
                     PLLLILTSYSMLGEQVTLWGLSGIVCVVGGTYSLYFKPGQHLLEPVKAVISDKGSLFM
                     VAVAFLYSITANFGKMGVVYSSPSFFGAVYFTILTGILCIWSMVVDNARHIFTKEIML
                     IGITQGIMITFHMLALEIAPVSYMISVKRSSMLFGIIFGSIFFGERNVVQHSIAALVI
                     LVGIGLISIFG"
BASE COUNT         4449 a         2671 c         2346 g         4242 t
ORIGIN      
        1 gggtggcaca gtgaatatgg atgaagtact gtacattggt ccttctattg catatcagtt
       61 ttcagataag tggagtacta cactggtagc cctggcaaca ccacaaaaat acacatggga
      121 aatgggaact gaaagaatgg aactgcgcag gtatgatgtt gatttagcat tgaactatca
      181 gattaaccgc tatataaaag tttttggcgg gggaaaatat ttagcttttg cataccacaa
      241 taaaacaaat gggaaagatg gaatacatca tgctttaggt cctgggggtg gcataagttt
      301 taccatgcct attataacca atgtatattt actggccaat ctttcgggat tatacattta
      361 tggaaaacag caggaagaac aaacaactgg agatcaaagt atcaattttt atgaaatagg
      421 tgccaatggt tctatacaat tagcctatta tattccttct gttgcagtta cccttgcagc
      481 agggtaccga tatcaatatg ttgaatctca ctatacagaa aacagctcag ctaatccaga
      541 tttagagcat acatttaaag gctttacagt attacttgta aaatcttttg atttataaaa
      601 atatgtaaaa catttgatac cagtaccata ttcagtgaaa tacggttgta atattttttg
      661 tgaaatacct gcatattttg ctactataaa aataaatata ttataaaaag aaaggctaac
      721 catgatatcc tttgaaggct acgatactgt aatcaaaaaa gtttatcaat caggcaatga
      781 gcacgtattc acgttttggg atgatctaac cattgagcag aaaaagcacc ttttacagga
      841 actatcgctc ataaattttg atgagataaa tgaattatat gctcttacca agaagcatat
      901 cattcctatg aattttgaac ctgctccttt cacccgtttg ccacaaacgc cggaagaaca
      961 caaagcattt gaatatgcta aacaggttgg aattgatacc atcaaaaaag gcaaggtggc
     1021 ctcatttgtg gttgcaggtg ggcagggaag ccgtttgggt tttgatggac caaaaggtat
     1081 attcccaatt gggccggtaa gcaataaaac gttatttcaa attcacgctg aaaagataaa
     1141 agcctatgaa aaaaaatttg ggattgatat accatggtgc atcatgacat cagaatccaa
     1201 tcaccaggca accgttgact attttgaaaa acacaacttc tttactttga ataaagataa
     1261 agtctttata tttaaacaaa atatgattcc ttcccttgat gagcagggaa aacttatttt
     1321 agaaacccca tattcaattt tcaggaatcc tgatggtcat ggtggaagtt taacggcatt
     1381 atacacatca ggtacattga ataaactttt aaccatgggc attgaagtgc tttcatattt
     1441 tcaggtagat aatccactgg tacgcatcat tgatcctgtc tttataggtt ttcatgttaa
     1501 acataatgct gaagtttcaa gtaaagcatt gcaaaaagct tatccggaag agaaaatagg
     1561 tgtatttgta aaattttcca atggcaggat aggtgttgtt gaatattctg accttccaca
     1621 ggaaaaacaa tacgaaaaag acgagcaagg taatttgcgt tatggtgcag gcagcatcgc
     1681 catccatctt tttaatacac actttattga aaaaatagca cagggaaata tttcactacc
     1741 gttccatgtt gctaaaaaat ccattccaca atacacaaat aatgggaaag ttactatcac
     1801 cggttataag tttgaaaagt ttgtctttga tgcgctacca ttaactgaac ataatttcat
     1861 tcttgaaact aaacgtgaag aagaatttgc tccggtaaaa aataagtcag gtgttgattc
     1921 tgttgatact gcacaggaac tgatgataaa tctttataaa aaatggctgc tacaaaaaaa
     1981 tattcctgtt ccaaaagttg ttgaaaaaat tgagatttcg ccattatatg ccgttgaacc
     2041 tgatgatttg cctgattcaa ttgcaatccc acaacaaaaa gaggtttacc ttgcatgaaa
     2101 cgatatattg aaaaagttaa tacacttatt gaatcattcc cctatatcaa agaattttac
     2161 ggtaaaacca ttgttataaa atatggcggc catgccatgg aaactgatga aggcaaacga
     2221 tcatttgctc aggatatggt tttgttaaaa tacgttggca ttaatccggt aatagttcat
     2281 ggaggtgggc cgcagatcaa tactctttta aaacaggttg gtgttgaaag ctcatttgtt
     2341 ggtggtatgc gagtaaccga tgctccaacc atggaaatag ttgaaatggt gcttgttggg
     2401 aaagtaaata aggagatagt taactatatt aacctggcag gtggaaaagc agtgggatta
     2461 tcgggtaaag atggcaatct tttcgttgcc cagaagattt accatgacgt gaatggtcag
     2521 aagctggaca ttgggcaggt gggtaccata cagcaggtaa atccgctgat tattgaaaca
     2581 cttgacagag aaaaattcat cccggttatt gcacccattg gctgtgatga gaatggcgtt
     2641 acgtataaca ttaatgcaga tattgcagca ggtaaattag ccgaagcact gaaagctgaa
     2701 aaattaatac ttttgaccga tgtcgaaggt attaagattg atgatacact tgtatcaacc
     2761 ctgcgtaaag aagaagtaga taatctaatt gttaacaata aaattaccgg agggatgata
     2821 ccaaaagtca actgttgcct gagcgcatta aaagagggtg ttagcaaaac ccatattatc
     2881 gatggcagag ttgagcatgc ggtactttta gaaatattta ctgatgcagg tattggaacg
     2941 gagataatat gaaattctaa tttacagata gactatatac ttttgtttct atatatgtat
     3001 atacgtcatt tcattaaact atatcaacaa taacaagtgt aaaatcatca tcaaaatttt
     3061 tatggggata aatccatcgg taggctgcag ccagtatttc attttttaat gcttttatac
     3121 ttttaccatt atattctctg ataatatcta taaaacgatc ttcaccaaat aattcacctt
     3181 ttgtatttcg taattcaacg ataccatcag taaataaaac aatcctgtca ccttttccta
     3241 tgttttcacg ggcaactgca aaaaaagcat ttggataaaa gccaatagca ccaccttttg
     3301 gccttagctg cacaatagac ccatcaactt tacataacac catagcatag tgtccagcat
     3361 tacttgtggc aacagtcatt ttttttgtat caacaactgc atatatggca gtgacaaatt
     3421 gattccctat cctctgggat aatgtatttt ctattgataa aagatattta tcaggaaatt
     3481 catcatactg acttgcatgt gcacatgcca ttttgaccat tgctgcgcca attgcggcag
     3541 gaatcccatg tccggatata tctgcaataa atattccaac ggtatcttca tttattaaat
     3601 ggatttcagc aatatcacca ccaataacct ctgtaggcat aagtatggtt tcaatggcca
     3661 catttgttaa ttcgggtatg ttagtctgaa caatagaatt atagattgtt cgtgctattt
     3721 caatttcatt ttttaaaatc acaagttttt cattttcatc ttttattaaa ttataacgat
     3781 accctaacgc tatcgataac aagatgacat caatactggt acccagatga aagccatagc
     3841 gcgtgataaa tacatcaggc agaataccaa aattacgtaa actgataagg ctcacaccca
     3901 aaaaaacggc aatcattgat gctaagtaga aatatgcagg cttgtattta ttttttaccg
     3961 ctatcacaca tgctataaat gcaagtaacg ttgccacaat tacttccgta ttgaggcaaa
     4021 taattgaaaa tgaaaggggt gcaagaggga tggcaataat tacagggaca attaatgcta
     4081 taaatatttt taaaagagta tcataccagt gtaaaaaatt acgggtcatt aagaaatttc
     4141 gtgtaaatag catgaaagaa ataatagcaa gatttcctaa aaatactatt gaaacactat
     4201 gaaaccatgg agagtcaggc cagaaaaatt gatatgcaat tccattgaaa ctgatattgc
     4261 tcaaagctga aaataataca tacagaatat aatacagata gctaatgtct cgcaaggtta
     4321 ttaataaaaa taaattgtac aaaaacattg ccatcattat gccaaaataa aaccacagac
     4381 caccatattc ttttatacta ttttctaata atgatgtata tgtccgtatt tgtaaaggga
     4441 tgataacctc acttgttgtc ttaacacgta taagatagtg agctttttca ttataaccca
     4501 gcgttagggg aagtataaaa ttattgagtg caattttcct gtgtccaaat gggaaaatat
     4561 cacctccttt tgcaatacat gtgatgcctt tttcattaac gttgtaaaat tcaaaataat
     4621 ccattgttgg gaatgataaa tccaccaacc agtgcgtatg ggatgatctg ttatgtacgg
     4681 aaaaataaaa ccaccatgaa gaatttgaat agcctttgtt aatgcctttg ctggtattat
     4741 cttgaaattt ttttagtaaa tcatttttta tgatatcctg agcagaaagc tgtgaggttg
     4801 gatcttcaaa catacttata tatggggagg tatcataggt atcgtgaata ttatcaagta
     4861 ctatagtacc cgcaaaagcc tgaatcgttg gtagtactat cgtaatcaca agagcaccta
     4921 tcaaggtttt aaacgatatt acatgccaaa cagtactatt gtttcccttt ctcatgatgg
     4981 atacaatgct tgttaacact atttaaaata ccctgtactt tatattcatt ttgattacag
     5041 cactatttct ttacaaatta cccatgattg taaattgatt aatacacatt gaatgatgca
     5101 caacgtacac ctgagaagct ggaaagtata catgccatta ttcattcaat cttttagtat
     5161 tgatgttagt cataataaaa aatcactcta tgttcagatt gtactcatgt caagggatta
     5221 ttataagaaa ggacagacgt tttaattttc ctcaatgcaa aaaaaacatt accacacctt
     5281 gacttttaat caatatttaa gtataatact atatactata atactacagg gggataacaa
     5341 tgcagaaaaa tatgggaaca attgacaggt tactgcgaac aataattgcc attgtaatca
     5401 ttgtgcttta ctttaccggg cagattactg gcactgcagc tattatactg ggaatacttg
     5461 ccgtggtttt tttattgacc agtgcagtaa gcttttgccc tttatatgtt ccactcaaga
     5521 ttaatactaa aaaacaataa tcgaattaac gttaacccaa acacaattca ggaactatga
     5581 ctatattatt gaatacccca aaaaaagggg tattcaataa tgtatgttat gcgggtttcc
     5641 caacaggaat aatatagagc ggctcttcac cttttcccag tgatactatt tctttaacca
     5701 tttcatcttg gaaagcccca accatgacac tacccaatcc cagtgcaaca gcctgtaaac
     5761 taatattctg gccagcatgc cctgcctcca tgtgtacata ccgtataccg cgctgaccat
     5821 aatgcccggt agtgcgttca tagatagcag ttattacaat aactattggt gcttcttcaa
     5881 taaacgtttg atgtaaagca gccctgcaca gggcactacg caaatcatag ggggctattc
     5941 gtttcaatcc atgatttttt acaatatacc gatacacccc tttttctaat tcttcaacat
     6001 tgccagctgc tacatagcag tttaagggat aggttgcacc tgctgatggc gcagtacggt
     6061 aacttccctg tgatatgcca ctggcagccc atagaatttg cgatagttcc tgcaaagaga
     6121 tagccatttt agaaaaacgc cgcaccgaac gacgctgttt cagtgtgtat tctattgaaa
     6181 catttccatc atacaaaggt cttggtaaaa taatatcatg tttcataact catatcctgc
     6241 aattaaatag cagacattca ttcttgggtt agcactcatt tcactgctta ataatagaaa
     6301 acattttttc actctaacag attaaaaata caaattgcaa cttgacaaat caacacatgc
     6361 atcaactgat ataattaaaa tattgatatg tacttccaat tctcacattt gtggcagttc
     6421 ataatgttgt attgtttttc attacgctat ttgatttcaa tttaattata ataactctca
     6481 ggaactatct catgaaaaaa atattttttg tagtgttact gttaactata actatacctt
     6541 tatttgccaa aatgcaatat gatgatgaaa gaatttttac ccgtgctgaa ataacgaaaa
     6601 ttgaaaaaca ccgcatagca ggtgatcaca attatcctct atacgaaaca aaaatccatg
     6661 ttactatcct tgatggcgta ttaaaaggac agacaaaggt tgctaccttc cggggagagc
     6721 atgacctgcc aaaagacatg ttctataaac agggggatgt cgtattcatt ggcatatcta
     6781 aaactggaat ggaaagcact cctgaatata tatcgctata cgatattgat aatacaacaa
     6841 tgatactaat cctcacagta atgctgatac tatccgtaat agtcattgga aaagtaaaag
     6901 gcttcttttc gctgatggct ttactcatta caatactctt actatttttt gtattaatac
     6961 ccttaacact taaaggctat ccccctttac cattagcgat agttatttca attatatcca
     7021 ctgtgataac attgccagta atagctggtt tcaagaaaaa gacatttgct gctatattag
     7081 gcgcatctgc aggaataatt atagcatcta tccttgcata tattgtggga aatataatgc
     7141 atctttcagg atttgttacc aatgaaatgc ttaccgtatt ttatgttgcc gacgtagcaa
     7201 ttgatatacg ggggctggca ttagccagta ttatcattgc tgctctgggt gcggtaatgg
     7261 atgtatgtat ttctatagcg tctgcaactg ctgaaatttt caatgcaaat ccagatttat
     7321 cacctaagga tgctttcagg tcggttttaa cagtaggaac tgatattttg ggttccatgg
     7381 tcaatactct ggtcctggcg tatgttggaa gctctctgtc attaatactt cttatctcca
     7441 tgcgcattca accaggaatg ccagtgtgga tgatatttaa ctacaatcca attttaagtg
     7501 agatagttaa atcaattatt ggtagcattg gtatgtttat atgcatccct atcactgcat
     7561 atatttcagt acatttatat ggacgaaagg atggcgctgc tagatgaatt tcaccccacc
     7621 atataaagct ggtggggtga aatagaagaa ctaccgttta aacaactcgt taatttcctt
     7681 ttcaaaattt ttcatgatga tattacgctt aactttcagc gttggcgtca tctctccggt
     7741 tgcctcagta aacttcctgt ttatgattac gtatttcttt attctttcat actcttcaag
     7801 ttcactgttt gccagctgga tatcctcatc aaccagtttt tttagttttt cattctgaat
     7861 aaagtcttcg ccaacaccaa tacacattct ggctgcgccc tcgccaaaga ataccagttt
     7921 tgatttatcg tatggtattc cttccttatc aaacagttca ataaaaacat caaatttagg
     7981 aacaaccagc gcgccaacaa atggcctgtc atcagcaatt gggaatacga tatcaacata
     8041 ccgcgagacg gaaaataaac tttctatctt tgcagcagga acatttttgc cagtatccag
     8101 aaccattatc cctttttttc tgtcaacaat cttgatatat ccatcaggcc ccatctgaac
     8161 aatatcacct gttttataga aaccatcttc agtaaaagct tctttcgttg cctcaggatt
     8221 attccagtaa ttcaggaata tattatcacc acgcacctgc cattcgccat cacttgccac
     8281 acgcccttct acaccattgc atactggccc aacatagcca ggtaatacct tatgcggcaa
     8341 attaagatta acagtattac atgtttcagt agcaccatac ccttcataaa tcctgacccc
     8401 cattgccata aacaccttgc acaaatctgc gggcaaactg ccagcagctg aaaacgcaaa
     8461 cctgaatctg ccgcccaatt tctcccgtac cttgctgaat acaagcttat caaccaattt
     8521 gtatttgaat gccagccacc ttccaacacc ttctgtaaaa tcaatgcctt cggacatatc
     8581 aacaaatcca tttttatctg cacgtgcttc tgtgagttgc aatccggttt taattgcaaa
     8641 atcaaagatc ttcttcttta ttggcgattt agctgccata tcgcgcattg ccatgaaaat
     8701 gcgctcatat attcggggaa ccgacataaa aactgttggc ctgaaaattt gcaaatcctc
     8761 aaccagcgtc ataggcgatg agtatgcaat ggttgcagca gccatcatag ctgtaccgtg
     8821 gccacattca cgttcatagg tatgtgcaag cggtaaaaat gaaagcaaca catcatcttc
     8881 acgatattcc ggaacaatac gcaaatccct gcagcatgct gcattgatac tgaaatgagt
     8941 atgcacagca cctttttgcc ttccagtggt gcccgatgta tagacaatag tcattgggtc
     9001 aagtatgtta accgatcgcc atcgccgctc atacgcaagc ctatcttgtg ccagcagttt
     9061 aatcccaaaa tcccttacct gctgtagcgt tacaacacgt gaatcattac cgttgtagtc
     9121 attgttcatg atgatgattt tttccagtgt tggcatttcc tgtattgcac tcagggcccg
     9181 taatagaatt tcttcatcct ggacatataa aattttagat ccggaatcat taataataaa
     9241 actcatttct tttgctgaaa gcgttggata tacacataca gtaatgccac cagcgcatag
     9301 tattgaataa tccgcccata accattgtgg tgcagtatgg gaagctatag caacacggtc
     9361 acctttttcc actcctaagc tcatcagacc agcagttaac tgttttatta taattcctaa
     9421 ctctgcatag gttatggatg ttgtagtatt tggaccggtt ttccaccatt gtgcacgccg
     9481 ggtaccaaac tgtattgcat tcctgttgag tatttcaggg aatgtcagtt gttgtatata
     9541 ccacttatgt atcataaaag cccccttata attaataaga aaatctggaa aatacatagc
     9601 atattttatt acgataatat atttatttgt atatgtcaag ttgaatatta taattattat
     9661 tttatacagc aaaaaaattt ttactgtttc actaattctg gaaaattgaa ataataacaa
     9721 tagattaatt gagtttattc tacatatcaa acatcttttt gccggtttat caaaatattt
     9781 attgataaaa ataaaatatt agtatgcaac atcctattgt ggtattactg tatattaatg
     9841 gtattattgt tcaatattcc tgatatatta cctgaacaac ggtgattgct actatgaaga
     9901 aactatctct tgctatcttt tttgtgataa caacagtttt cataatcatt cagtgctcct
     9961 gcagttccag ccaaatgatg gatgcacaac ccatacgttt tgtggttacc agcaacacat
    10021 cgccggattc gccctatgag gagttcaatc ctcagcttac taacacactt ctatcaatca
    10081 ataaggaaaa cccagtattt accatgcatt gtggcaacat cattcatggg ggctcatcta
    10141 tcatgggtat taatgtaatt gatgttgaaa aacaattcca gcaatttttc aaatttgcaa
    10201 agaaacttaa ctgcatcctc tttactgcat gcggtccgct tgatacatat aatgattcta
    10261 cagctatcta cacaaaattt accgggaagg atgcatggta ttcttttaat tatggcacca
    10321 atcacttcat tgtattatac agcaccagca aaaaaacact ttccatttca gacaggcaac
    10381 tgtcctggct aaaagaagat ctgaaaaagc attcccatgc gcatatattt gtcttttcat
    10441 actttttgcc agatattcag cgcacaaagc agcagtttgc aaatgattca caccagaaac
    10501 ttcatgaact tttgtcacag tatactgtta aagctttttt tggcgtcaca gggcttactc
    10561 aaccagtaac tatcggcact attcaatata ttccagttcc ctgtgacaat tcgtatacca
    10621 ataaatataa aaaagcaata cgctattatg tcgttgactt tgacgggcaa tacattaccc
    10681 ttgtaccacg atatttttaa atcatataag gtcatgccat gcaacgtgaa ataaaatttg
    10741 attccataca tgaatttatc aatgcacttc atgccagcaa cattactaaa gcagcgttta
    10801 cacagattaa cgaaaagcgt gcagttgaag ttaaacccgg cacattacag gttgtagatg
    10861 ttgttattgc tgaaatactt gcctataaag atgcaaccat ttacaaatgc aagattgaaa
    10921 atgctgattt agatgacctc tatacaatgc tggaagatga aggttttgaa ttaacaaaac
    10981 gaagcaggaa tattacataa tcatggaagc gatagttacc attgaaaacc ttaccatttc
    11041 atttacaaat ctttatggta aaagctatgc agtacggaat ctgtcactgt ccataccaaa
    11101 aggcaaaacg ttgggccttg tcggtgaatc agggtgcggt aaatccgtaa ctgcatattc
    11161 tatccttaat ttagtgccaa agcctggcaa aattgaatct ggtactatca catacaattc
    11221 cattgatctt ttgcattgtt ctgaaaacac actgaggtca atccgtggga aaaatatagc
    11281 catgatattt caggaaccca tgacatcact gaacccggta tttaccattg gctatcaggt
    11341 gcaggaagca gtggagcttc atttgcagta cagcaaacag gaggcaaaag agtacaccat
    11401 tgaactttta ggcaaagctg gcattcccaa cgctgcacaa cgctactacg catatcccca
    11461 tgagctatcc ggcggtttgc gccagcgagc tatgattgca atggcgcttg ctgctcatcc
    11521 agatctttta atagccgatg aaccaaccac cgcgcttgat gttaccatac aatctcaaat
    11581 actggattta ctgcttacac tgcaggctac agaacatatg tccatgcttt taataaccca
    11641 tgatcttggt atagtagcaa atactgcaga ctatattgcc atcatgtatg ccggtgagat
    11701 tgtggaatat gcacccacaa aagagatttt tgaaaaccca ttacacccct acactcatgg
    11761 gcttatgaac tgtattccgt cattgcataa aactgcaaag cgattatata ccattccagg
    11821 caatgtaccc attattacac aaccaccaca gggttgcgtc tttcatcccc gttgttcttt
    11881 gggtgatgat gaatgtctac aaaaacccgt taaccttgct ggaagaaaaa aagaccatct
    11941 tgtgcggtgt attaaagtgt agatatataa agaggggcag gttttaaacc cacccctttt
    12001 aagtttaagc ttttaatatg ccagattgat atggtaggtt ttcttgatgc cgtttaagat
    12061 tgtctgaaat ttgtttggat ccttttcacg agtggtcttt aacaaatcaa caacttcagc
    12121 tcttgtcaac acaacatcgt aatgttcgca atgagccaat gactccctgc cacatatatt
    12181 tctgttaatg catgatgaac atatagaatt tcccattgtg tcctccttat ctataaacat
    12241 atcgtttata ttcaaatagt atgtgcttaa tatataagca agtaccgtgc caattcagta
    12301 aaacctgaat gtatttttta ttttatggga gtttttaacg gttttatggc aaaattttta
    12361 ctttttttgc tcaataatta cctttaagcc agaattatgc cataactaat gtttaatatt
    12421 tgggcgatag ttactgtaaa ttgaacattt ttaataatag tgtccataat tttacagtta
    12481 ttactgcctt tgtttgttga atattaatat cagtgtatca tttttacaac atcatccgaa
    12541 tatactgata aggccaatac ctaccagaat caccagtgca gcaatactgt gctgtactac
    12601 atttctttcg ccaaagaaaa tagaaccaaa gatgatgcca aatagcatgc tggagcgctt
    12661 tactgaaatc atgtagctta ccggagctat ctccagagct agcatgtgaa atgttatcat
    12721 tatgccctgt gttatgccta tgagcataat ttcttttgta aatatgtgcc tggcattatc
    12781 caccaccatg gaccatatac ataaaatacc ggtaagaatt gtaaaataca ctgctccaaa
    12841 aaatgaaggg cttgaataca ccacacccat cttgccaaag tttgccgtta tgctatacaa
    12901 aaatgccact gcaaccataa acagcgagcc tttatcggaa atgacagctt taaccggttc
    12961 aagtaaatgt tgcccgggtt taaaatacag gctatacgtt ccccctacca cacatacaat
    13021 acctgacaat ccccatagtg taacctgctc accaagcata ctatatgagg taagaatgag
    13081 taaaagcgga gtgaacgcta aaaacggcaa tgtgcgatac atcggtgaga tctgcaatgc
    13141 gcgcatatac agtaccagcg caaccacctc cagtggcgga gaaaatataa agacataggc
    13201 aatacgccag tcaacagcag ggatcccttt gataagagct atcgcccaca gtactggtaa
    13261 agaaaaaaat atgcgcccgg cagccactgt tactggatga atctttggag catttttaac
    13321 cagcacatca ccaatggcta aaaagagcgc gcaaagcagt gcataaataa cccagataaa
    13381 tcgcctcctg tgcaactata ctataattac tttgtcattt tgtatctttc caatactttt
    13441 tatttaaaaa caaatctcat atggtgaatt atttactatg cacctaatca tagcactttt
    13501 tactacaaaa atataatttc atgcacaact ttcataacca gttccgtcaa acaacttttt
    13561 ttaccatact atgtattgtc ttaggctctg accctatttt ctgtcaattt tagactctga
    13621 ttttttatgc ttaacttatc aaaatttctc tgagatctct tctttataca taatttttca
    13681 attcacgcag agtgcgtcta tggtaaag
//
LOCUS       DAORYL010000078        13687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_3535_length_13687_cov_12.172682, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000078 DAORYL010000000
VERSION     DAORYL010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13687
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3535_length_13687_cov_12.172682"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(571..972)
                     /locus_tag="PLV81_12240"
     CDS             complement(571..972)
                     /locus_tag="PLV81_12240"
                     /inference="COORDINATES: protein motif:HMM:NF014599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HOM10912.1"
                     /translation="MDKDKQPGIKIDAILLVESFFKRTPNVSLPIPLNIKFSINNTID
                     KEKKQLVTEMKVMLNSEKDSVYAYVVYVGIFSTADDENMSLEDFAEKIAPAHIYPYIR
                     EEIHIRSIKAGLPPIIIQPLNFMAKSVKKQN"
     gene            complement(975..1385)
                     /locus_tag="PLV81_12245"
     CDS             complement(975..1385)
                     /locus_tag="PLV81_12245"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOM10913.1"
                     /translation="MPKYNIFDEYIAKDVKEKHNELMAQAKALFNASNILSEVINSLN
                     KSQKEIAECLNISEGYLSRLLSGTENISLKNFAKILFKLGYELQLSGKKFSLENQKNM
                     VWVNFGESLEPDSISIHSSKRQNEWGNSTYQKAV"
     gene            complement(1390..1857)
                     /locus_tag="PLV81_12250"
     CDS             complement(1390..1857)
                     /locus_tag="PLV81_12250"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOM10914.1"
                     /translation="MTLEPKYKYGGILIVEADKKSGEKFDIIAAQLESGNCPFWDYFC
                     GLKHKYEESLRKRISLKKDDQINFSKLHQYFDKFCRTGHWYNERQIKKIEDGFFEFKI
                     METGLRVIFYYDELNKGVIILTHTFLKKKDKMPQKERERMYFIKDSFEKMRRR"
     gene            2353..4251
                     /locus_tag="PLV81_12255"
     CDS             2353..4251
                     /locus_tag="PLV81_12255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOM10915.1"
                     /translation="MEQNTAKLEDFKRMYPEKFLPEEKIFSHINRGDRIFIGTACSEP
                     QYLVNALVEYVKSYPKAFFDTEVIHVWTLGVAPYADEKFKQNFRHNSFFIGNNTRDAI
                     NQGIADYTPIFLSHVPNLLRKGIIEIDVALVQTSLPDEHGFLSLGVSVDIVKTAVESA
                     RLVVVQVNKYMPRVHGDAFIHIKDVDFIVPYDEPILEFTSKVSDEISNRIGKYVSLII
                     QDGDTIQVGYGRMPNAILKHLITKKNLGVHTELLTDGIVELMKAGVITNSEKTVDRGK
                     TIASFCMGTKATYEYIHDNPSIEFKTIDYTNNPLIIAQQRRMTAINAALQIDLTGQAT
                     AESLGKMFFSGIGGQADFMQGAVLAPGGKTILTLRSTTENEQESRIVPFIPEGAGITL
                     NRGDVHYVVTEYGIAYIHGKNIRERAMDLIAIAHPKFRPWLIEEAKKNGLIYRDQAFI
                     PGKKGEYPEHLETYRTTKTGIKLKLRPVKISDEELLKDFFYSLSDQSLYRRFISTRKD
                     MPHERLQEMVVIDYTKEMVILAVIEQNEKEVVVGVGQYGIQEFQHWAEVAFAVRDDYQ
                     YKGIGQVLLSYLTQIAKRNGLLGFTAEVLVENKPMLHLFEKMGFNIEKRSSAGVYELK
                     MEFRGDEQ"
     gene            4248..6365
                     /locus_tag="PLV81_12260"
     CDS             4248..6365
                     /locus_tag="PLV81_12260"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOM10916.1"
                     /translation="MKAKKDSIVSLFQPKSVAVVGASSQPGKIGFSITRNIIQGGFKG
                     KIYPINPKGGAILGVDCYPSISDVKDEIDVATICVPAKLAFDAVKECADKGVKHVQII
                     TSGFSEVGEVELEHKIVEYANSKGTRVLGPNIFGVYSASGNYNSTFSATDILRGNVAI
                     LTQSGALGIAMIGKTAVENMGLSAIVSLGNKADIDEADCLDFVMRDQETKVILMYIEG
                     VKDGERFIEAVREATKLKPIVVLKSGRSKRGAMAAASHTGSLAGSDEIFDAIARQTGL
                     LRAESLQEAFNWCKFLAYSPRPKGKNTVIVTNGGGIGVMATDACEKYGVELYDDQLVL
                     KEVFGPATPDFGSTKNPVDITGGAKAADYDLALTAPTRSEDMHATLALYCETATFDAE
                     NLAPMIRDTYGKHLASGKPVAYAIVGGKVVEDAVVQLRKMNVPVFPEVYEAVSCMGAL
                     YKYTNMLAERDDSVEDAQMDIATINKIIDGALADGRTFLLANEGQAVMKASGVMIPGA
                     GIGKSIDECVRLAEQIGYPVVMKVVSRDILHKSDAGGVALDILNKQEVMDAYEAIMKN
                     CRAYKEDAVIDGIEVCEMVQKGVELIIGARRDPQLGPIVMCGLGGIYVEVMKDVAFRA
                     ASINKKEARSMLSEIRSYPLLLGVRGEEQKDIDIVVDTIIKVATIIRKIPRITDIEIN
                     PVVVYEQGKGLKAVDVRIIISKS"
     gene            6379..7410
                     /locus_tag="PLV81_12265"
     CDS             6379..7410
                     /locus_tag="PLV81_12265"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOM10917.1"
                     /translation="MNKIVFACTRRSAGKTTIFTGLAKAMGKEVAYMKPFGDRLIYRK
                     KRLWDYDAALLTNLFGLEENPEDMTIGFEHAKLRYVYDEARIKEKLQEVAKSIAKNRE
                     VLFIEGGKDLAYGVSVYLDPITVAKSLDAKLVLIVGGNDDVIIDDITLFKKYVDTSDI
                     NFLGVIINKIHDLDDFKSTYADKIEKMGVPILGMIPYAADLAYFSMDYLSEALFAKVI
                     AGEAGLNKVVKNVFLGAMSVNEALRNPLFNKEGKLLITSGDRSDIIISALETNTVGII
                     LTNNILPPSNIISKAAEKNIPLLLVSSNAYQVAGQIDKMEALLTKDNKEKIALAEKLV
                     KENVKVSAL"
     gene            7696..8064
                     /locus_tag="PLV81_12270"
     CDS             7696..8064
                     /locus_tag="PLV81_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10918.1"
                     /translation="MATPYMLQNMYDSSVYLCQERIWHQIIDTAFQRGFQPVGTRLDY
                     YYELDLVWDTETTFMEKIFTSIMTHARCLNWNKYNFKDRENQIVCDEDCSELLYVLQD
                     ILPQDLKDFFSKGSFRICSD"
     gene            8097..8819
                     /locus_tag="PLV81_12275"
     CDS             8097..8819
                     /locus_tag="PLV81_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOM10919.1"
                     /translation="MAIVEWKKDENIAIITMNTTENRHNVMFAQAMLQALDEIEADKE
                     INGIVLTSSDAKNFSLGVDIEWLMPRMAEKDFKAIKDFMYGMNNVFKRLLLIPMPVVA
                     AINGHAFGNGAILSCACDFRFMRSDRGFFCFPEVDLGIPFLPGMIAFVKKAIPYYKFN
                     EMKLTGRRVGAPELAEHHVIEKACANVEELMKESIAFAKSFKKKRGIFGEHKKRLHKH
                     IIEVIDKEDPEFIESLFLFIQD"
     gene            8964..9881
                     /locus_tag="PLV81_12280"
     CDS             8964..9881
                     /locus_tag="PLV81_12280"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOM10920.1"
                     /translation="MSLDPSVKKVLELLKNIELSSLTVEQARKLMDMGIDRQIKEDVK
                     STSDFKITYNDISLSCRLYEPFTTTDALIIYYHGGGFMFGNIELFDHVCRKATNSSGC
                     KVISVEYRLAPEHKFPAAVDDAYHSFRWIKDHAKNFDINPSKIAIAGDSAGANLATVT
                     CLRCKDTSMDLPKLQVLFYPVVAPDVCSESVRQFGDGFFLTRASMKWFTQLYLSSPAD
                     FINPYMFPIFHQNPAGIPEAIIITAEHDPLRDQGEMYNLFLREAGIQSTCIQAKGMIH
                     GFLNFSYLIPSAVSIADMVWCVVGKKLVV"
     gene            complement(9887..11317)
                     /locus_tag="PLV81_12285"
     CDS             complement(9887..11317)
                     /locus_tag="PLV81_12285"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOM10921.1"
                     /translation="MSKKFIALVVIGVIIAGVVIYRAMAPATIDNAIEYIDSGNYKKA
                     LKVLNTLAKTSGYEDGERIYYYRLKALLGFINELNEDYADECKEIQKTKNKKIESKLK
                     AKLEEINKETEADLILVTDPECYISMGGTLYDEFVSLYPGSRYIESIDFDMLQFIMSK
                     HPGTINPVIEFYKRYPETTYLSSLVNMVLKALGNPKIELKGHAEKLTEMIQNFCNKYH
                     TSAEYYRLFRCKGENVNLRDSAGTHGQITGKLNKGEFVIQLERSMDSVQIGDVRDYWY
                     RVVSVSGSRGWVFGKFLERIEPMTKNLVSEDEGFTIDEHFSEWIDSHTPKGWQHMYPG
                     FEEAISFTKLKDIHIVKLDSGEKGGLYKKTGSFQNMVCKVKGRLLSGNVILAGVVGYG
                     GLAAVITLLPEEVDVCGYKIPYTTTQWHEYELRSEGKTLSLFIDGELVARKINTKKHD
                     LLKSSGIYILVCSTKTQAEIEYIRIK"
     gene            complement(11325..12536)
                     /locus_tag="PLV81_12290"
     CDS             complement(11325..12536)
                     /locus_tag="PLV81_12290"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOM10922.1"
                     /translation="MSERKIAILIGDGMADYPIEELGGNTPLQYAKTDHMDYLAKNGI
                     CGLARTVPSGMPPGSDVANLSVFGYDPATCYTGRAPLEALNMGINLGPDDVAFRCNMV
                     TIEHDIMNDFAAGHIDSEFSKIVIDEIKNTIHRDGVEFYAGVSYRNIMVVRNFKGELP
                     ITTPPHDIQGKKIHEYLPKGANSELLNDIMQKAAGAIAHSKKIADAKLKFKGNPTGLW
                     LWGGGFRPKMEPLSTRFGLHGHTIAAVDLIHGIGRAAGLTPIHVEGATGYIDTNYSGK
                     AQACIDAMKNANFVFLHVEAPDESGHEGNLEHKLKAIEDFDAKVVGPVVNKLNRYGNF
                     TVLVMPDHPTPLSLRTHSSDPVPFVIYAPDMNYRFNCKGFNEVDARDTGIFVEHAHEL
                     LAMIVNNVYPW"
     gene            complement(12529..13287)
                     /locus_tag="PLV81_12295"
     CDS             complement(12529..13287)
                     /locus_tag="PLV81_12295"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOM10923.1"
                     /translation="MIALITDFGINDPYVGVVKGVIYSQGYTGSIVDICHHIVPYSIE
                     HAQYILTTSYRYFPDNTIFLVVVDPGVGTQRNILLIHDDRYYFFMPDNGIAGCLNQNT
                     IKVWSVDTQEFVHASSTFHGRDIFAMLASRLAMQGIEAIHCKECTNYVQHPFPWYDIK
                     VDTVYTRVVHIDRFGNCILSLPNEQYSLQLNAIIVNNKRYTLYHCKTYADVPLEAIGI
                     LKGSSGFIEICMNQKDASKRLAVTTGDMVELLYE"
BASE COUNT         4301 a         2573 c         2540 g         4273 t
ORIGIN      
        1 ttatttcctg ccataacttc acttttccta ccctaattat tcatattatc cttaagccca
       61 atttttccct tatccttcaa tgatacacag tatttcatac aatatgaata tgctgaaaat
      121 atagtttaat tttcccccaa gtgtcataaa tattatcatc actttcagga gtgatggaga
      181 tactacgcga aacgaaaact taggtatgtt ttgcaaatat agcattttca cacgcagaga
      241 aaaaataaga aacgaactgg ctgaagaagg tacaggatct tgtttcatgg gagtatttca
      301 aaaaaattca acaaattcta ttcagaacac aaagaccaac cagcgtggta cccgctgggg
      361 ttgtttcgct gattccttat tgttgaatgg aatacatcaa tcgaacgtca aagggaagaa
      421 atacttgagt ttcggtatga ttttagcaaa tttgcttgta cacctctaga ataggaagca
      481 cctgattcaa cgatgttttt agtatttcat aatctgattg aacatctgtg gcaagaatag
      541 cttgaggaga ttaccaaatt aatcatcaga ttagttttgt ttttttacac tctttgccat
      601 gaaatttaat ggctgaataa ttattggtgg aagaccagct ttaatcgaac gaatatgtat
      661 ctcttctcta atgtaaggat aaatgtgtgc aggtgctatt ttttctgcaa aatcttctaa
      721 agacatattt tcatcatcag cagtcgaaaa aatacccaca tatacaacat aagcataaac
      781 tgaatctttt tcactattaa gcataacttt catttctgtt accaactgtt ttttttcttt
      841 atcaatagta ttattaatgc taaactttat gtttaatggt atgggcaatg atacatttgg
      901 tgttctctta aaaaaagatt caacaagtag aattgcatca atttttatcc ctggttgttt
      961 atctttatcc ataattaaac tgccttctgg taagtactat taccccattc attttgcctt
     1021 tttgaactgt ggatagaaat ggaatcaggt tcaagcgatt ccccaaagtt tacccatacc
     1081 atattcttct gattttctaa actgaatttt ttaccagata attgcaactc atagccaagt
     1141 ttaaaaagta ttttagcaaa attcttaagt gatatatttt cagttcctga tagaagccgt
     1201 gagaggtatc cttcgctaat gttaaggcat tctgcaattt ctttttgaga tttatttaat
     1261 gaattaatca cttcactcaa tatatttgaa gcattaaata gcgcttttgc ttgtgccatc
     1321 aattcattat gtttttcttt aacatcctta gcaatatatt catcaaatat attatatttt
     1381 ggcataattt cacctccttc tcattttttc aaagctatct tttatgaagt acatcctttc
     1441 cctctccttc tgcggcattt tatctttttt ctttaaaaat gtatgagtca atattatcac
     1501 acccttattc aattcatcat aataaaaaat tactcgaaga ccagtttcca taattttaaa
     1561 ttcaaaaaag ccatcttcaa tcttttttat ttgcctttca ttataccaat gccctgttct
     1621 acaaaattta tcaaagtatt ggtgtaattt gctaaaatta atttgatcat ccttttttaa
     1681 ggagattctc ttcctaagag attcttcata tttgtgtttt aatccacaaa agtaatccca
     1741 aaatggacaa tttcctgatt ccagttgtgc agctattata tcaaactttt ctcctgattt
     1801 tttatcagct tctacaatta gaataccacc atacttatat ttcggttcta gagtcatttt
     1861 agtttaccta taagtaaatt caagaaaaat tttttaaaat attaaaattt cgtataatgc
     1921 aaatatctat taaatttaga tatttaacat gtccttataa attttgcaag aatcataata
     1981 aattatgtca ctaaaaacca taatttttat gtaattataa aaaatataat taacgaaaag
     2041 ttacagattg actaaccaaa atgattatat tatttatcta tttaacaggc tacataatct
     2101 gattcaaatt caaacaaatt ttttgaaaca ttttgaaagg tagttttgtt gtttggatat
     2161 cttgtttatt ttgtatttct atcttgactt atcaaaaatt gatagataga atattaatat
     2221 aattgtcaat ttttgaacat catttatgat atattatatt tagtataaaa atttgaattg
     2281 acagaattac tagctaaaat attttacata caatctgctt tactgtaagt ttagcagatt
     2341 gaaagggggg ttatggagca gaatactgca aaactggaag attttaaaag gatgtatccc
     2401 gaaaagtttc ttccagaaga gaagattttc agccacatta acaggggcga ccgaatcttc
     2461 attggaacag cctgcagcga gccccagtat ttagtcaatg cactagttga atatgtaaaa
     2521 tcatacccca aggcattctt tgatactgaa gttatccatg tgtggacgtt aggcgttgcg
     2581 ccttacgctg atgaaaaatt taaacaaaat ttccgacata attctttttt tattggcaac
     2641 aatacccgtg atgcaataaa tcagggtata gccgactaca ctcctatttt cctttcgcat
     2701 gtgcctaatt tgctgcgtaa aggcatcatt gagattgatg ttgcactggt tcaaacatca
     2761 ttacccgatg aacatggttt cctcagctta ggtgttagtg ttgatattgt aaaaacagca
     2821 gtagaaagtg cacggcttgt ggttgtacag gttaacaaat acatgccacg tgttcacggt
     2881 gatgcattca ttcacattaa ggatgttgac tttattgttc cgtatgatga acccattcta
     2941 gaattcacat ctaaagtgtc tgatgaaata tccaaccgta ttggcaaata tgtttcattg
     3001 attattcagg atggtgacac tattcaggtt ggctatggca ggatgcccaa tgctatatta
     3061 aagcacctta tcaccaaaaa gaatttaggc gttcatactg aattgcttac cgatggcatt
     3121 gtggagctta tgaaagcagg tgttatcacc aattctgaaa aaacagtgga tagaggaaaa
     3181 acaattgcct cattttgcat gggcacaaaa gcaacctacg aatatatcca tgataatcca
     3241 tccattgagt ttaaaactat cgattacact aacaatccgc tcatcattgc gcagcaacgc
     3301 aggatgactg caataaatgc tgctttgcaa atagatttaa caggtcaggc cactgctgag
     3361 tcgctgggca aaatgttttt cagcggcata ggtggtcagg ccgactttat gcaaggtgcg
     3421 gtacttgccc ctggtggcaa aactatcctg accctgaggt ctacaacaga aaatgaacag
     3481 gaatcacgta ttgtgccatt tataccggaa ggtgcaggca ttacactcaa tcgtggtgat
     3541 gtccattatg tggtaaccga gtatgggata gcctatattc atggcaagaa catcagggaa
     3601 cgagctatgg atttaattgc gatagctcat cccaaattca ggccatggct tatagaagaa
     3661 gccaagaaaa atggtctcat ctatcgtgac caggctttca taccgggtaa aaaaggcgag
     3721 tacccggaac accttgaaac ataccgtacc acaaagacag gcatcaagct taaactacgt
     3781 cctgtcaaga tcagcgatga agaactctta aaggactttt tctattcact atccgaccag
     3841 agcctctaca ggcgctttat atcaacacgc aaagacatgc cccatgagcg gcttcaggag
     3901 atggtggtaa ttgattacac caaagagatg gtgattttag ctgtgattga acaaaatgaa
     3961 aaagaagttg tggtcggcgt tggtcaatac ggtatacagg aatttcaaca ctgggcagaa
     4021 gtggcatttg ctgtgcgtga tgattatcaa tataaaggca ttggtcaggt actgttatca
     4081 tatctgactc agatagcaaa gagaaatggt ttgctgggat tcactgcaga agtgcttgtg
     4141 gaaaacaagc ccatgctgca tctatttgaa aaaatggggt ttaatataga aaaaaggagt
     4201 agcgcaggag tatatgaatt gaagatggaa tttaggggag atgaacaatg aaagcaaaaa
     4261 aggatagtat tgtttcttta ttccagccca aaagtgttgc agtagttggt gcttcatccc
     4321 aaccgggcaa aattgggttc agcatcaccc gaaatatcat tcagggtggt tttaagggta
     4381 aaatttatcc aataaacccc aaaggtggtg ccattttagg cgttgattgc tacccaagca
     4441 tttcagatgt taaggatgaa attgacgtag caacaatctg tgttcctgca aagttagcat
     4501 ttgatgcagt taaggaatgc gctgataaag gcgtaaaaca tgttcagatt attacctcag
     4561 gcttctccga agttggcgag gttgaactgg aacacaaaat tgtggaatat gccaattcca
     4621 aaggaacacg ggtacttggc ccaaacatct ttggcgtata ctctgcatca ggtaattata
     4681 attcaacatt ctctgccaca gatattttac gaggcaatgt tgccatcctt acccagagtg
     4741 gtgcactggg gatagcaatg atagggaaaa ctgctgttga aaacatggga ttatccgcta
     4801 tcgtatcact gggtaacaaa gcagatattg acgaagcaga ctgtttagat tttgtcatgc
     4861 gcgatcagga aacaaaggtt attttgatgt acattgaagg cgtaaaagat ggcgaacgtt
     4921 ttattgaagc tgttcgcgaa gcaacaaagt taaaaccaat tgttgtatta aaatcaggcc
     4981 gatcaaaacg aggtgcaatg gctgctgcta gccatactgg ttcgctggct ggttcagatg
     5041 aaatttttga tgctattgca cgacagacag ggttactgcg tgctgaaagc ttacaggaag
     5101 catttaactg gtgtaagttt ttagcatatt caccacgccc caaaggaaag aatactgtta
     5161 ttgttaccaa tggtggcggt attggtgtta tggcaactga tgcatgtgaa aaatatggtg
     5221 ttgaactgta tgacgaccag ctagtattaa aagaagtatt tggtccggca acacctgact
     5281 ttggttcaac caaaaatccg gtagacatta ccggtggagc aaaagctgca gattatgacc
     5341 tggcactcac cgcaccaaca cgttctgaag acatgcacgc aactctggca ttgtactgtg
     5401 aaacagctac atttgatgct gaaaacttag caccaatgat cagagatacc tatggcaagc
     5461 atttggccag cggcaagcct gttgcgtatg cgatagttgg tggcaaagta gttgaagacg
     5521 cagtagttca actgcgtaaa atgaatgttc cggttttccc tgaagtatac gaagctgtat
     5581 catgtatggg tgcgttgtat aaatacacca acatgttagc cgaacgtgat gacagtgtag
     5641 aagatgcaca gatggacata gctactatta acaagattat tgatggcgca cttgctgacg
     5701 gaagaacatt ccttcttgca aacgaagggc aggctgtaat gaaagcttcc ggcgtcatga
     5761 taccaggcgc aggtattggt aaatcgatag acgagtgtgt acgattggct gagcagatag
     5821 gttacccggt tgttatgaag gttgtttctc gtgacatctt acacaaatct gatgctggcg
     5881 gcgttgcatt agacatattg aataaacagg aagtaatgga tgcctatgaa gctattatga
     5941 agaactgtcg cgcatataag gaagatgcag tcatagatgg cattgaagta tgcgaaatgg
     6001 ttcagaaagg tgttgaactc atcataggtg cccgcagaga cccacagtta ggtccaattg
     6061 tcatgtgtgg tctgggtggt atttatgttg aggtcatgaa agacgttgca ttcagggctg
     6121 catcaatcaa taagaaagaa gcacgctcaa tgctttctga aattcgttca tatccattac
     6181 tgttaggcgt tcgcggtgaa gagcagaaag atattgatat tgttgttgac acaattatta
     6241 aagttgcaac aatcataagg aagataccgc gaatcacaga tatcgagatt aatcctgtcg
     6301 tggtctatga gcagggcaaa ggccttaaag ctgtagacgt caggataata atttctaaat
     6361 cgtaagtgag gtgtaattat gaataaaata gtatttgcgt gtacacgaag aagcgccggg
     6421 aaaacaacca ttttcactgg tctggcaaaa gcaatgggca aggaagtagc atatatgaag
     6481 ccctttggcg atagactgat atacaggaaa aaacggttat gggactatga tgctgcactt
     6541 ctgactaatc tttttggcct ggaagaaaat cctgaagata tgaccattgg atttgagcat
     6601 gccaagctgc gctatgtgta tgatgaagcc cggattaaag aaaagcttca ggaagtggca
     6661 aaatcaattg ccaaaaaccg tgaagttctc ttcatcgaag gtggcaaaga tttagcctat
     6721 ggcgtttcag tatatcttga cccaataact gttgcaaaat cacttgatgc aaagcttgtt
     6781 ctcattgttg gtggcaatga tgatgtcatc attgatgaca ttaccctttt caaaaagtat
     6841 gttgatacaa gtgatattaa cttcctgggt gttatcatca acaagataca cgatcttgat
     6901 gattttaaga gcacctatgc tgataaaatt gaaaaaatgg gcgttcctat tttaggaatg
     6961 attccctatg cagcagatct tgcctacttc tccatggact atctgtcaga agcattgttt
     7021 gctaaagtta tagctggtga agctggctta aataaggttg tgaagaatgt atttcttggt
     7081 gcaatgtcag tcaatgaagc actgcgaaat cctcttttca ataaagaagg caagctcttg
     7141 ataacaagcg gcgacagaag tgacatcatc atttctgcac ttgaaacaaa tactgttggt
     7201 atcattttaa ccaacaatat tctgccacct tcaaacatca tttcaaaagc agctgaaaag
     7261 aacatcccgc tgcttttagt ttcaagcaat gcttatcagg tagcaggcca gattgacaag
     7321 atggaagctt tattaaccaa agataataag gaaaagattg ctctggctga aaagctggta
     7381 aaagaaaatg tgaaagtttc tgctctatag acgcaattga cttttacaaa aaaaaggggc
     7441 tacaaaagcc cctttttttt gcatttattg taatttttag attacactgt gccatcaaca
     7501 taacagccat gaagatagtg attataatca tctttccctt acttctcaat acaattatga
     7561 taaggatgtg actgtagtat ataattaaag aactcatcaa tttaattttt tgtcggtata
     7621 aagttccttg acagggcata acgttgttat gctatagtaa ttggagttta tcaaaactat
     7681 accaatgtgt cagatatggc aacaccatat atgttacaaa atatgtatga tagctctgtg
     7741 tatctgtgcc aggaacgtat ctggcatcag ataattgata cggcctttca gcggggattt
     7801 cagcctgtgg gtacacggct tgactattat tacgagttag atctggtctg ggatactgag
     7861 accacattta tggaaaaaat atttacatct atcatgacac atgcacgctg cctaaactgg
     7921 aataagtata atttcaaaga cagagaaaac cagatagtat gcgatgaaga ctgcagcgaa
     7981 ctattgtatg ttttacagga tatcctgccg caggatttaa aagatttttt ttccaaaggc
     8041 tcttttagaa tatgcagtga ctaataacta aatttaaaac taaagggggt acttatatgg
     8101 ccattgtaga atggaaaaag gatgaaaaca tagcaattat caccatgaac acaacagaaa
     8161 accgccataa cgtgatgttt gcacaggcaa tgttgcaggc tctggatgaa attgaagctg
     8221 ataaggaaat taatggtatt gtgcttacat caagtgatgc aaagaatttc agtttaggtg
     8281 ttgatattga atggctgatg ccacgtatgg cagaaaaaga tttcaaggca atcaaagatt
     8341 tcatgtatgg catgaataat gtgtttaagc gcctgctgct tatacccatg ccggttgttg
     8401 cagccataaa cgggcatgct tttggcaatg gagcaatcct ttcatgtgca tgcgatttca
     8461 gattcatgcg ctccgacaga ggatttttct gctttcctga agttgattta ggaatcccat
     8521 ttttgccagg tatgatcgcg tttgttaaaa aagctattcc ctactataaa ttcaatgaga
     8581 tgaaactcac cggcaggcgt gtaggagcac ctgagcttgc cgaacaccat gtgattgaaa
     8641 aagcctgcgc caatgtagag gaactcatga aggaatcaat tgcatttgca aaatcattca
     8701 agaaaaaacg tggcattttt ggcgagcata aaaaaaggct tcacaaacat ataatcgaag
     8761 ttatagataa ggaagaccct gagtttattg aatcattgtt tttatttatt caggattaac
     8821 acttcaaatt aacattaata ttgaaaaccc tcgcttccca tagtgagggt tttttaatat
     8881 aaaaaatttc gttgacatat taatgtccat tcattaattt ttacagtaac tatcgcacac
     8941 aaaaaaacag ttggaggtct tcaatgtcat tagatccttc tgtaaaaaaa gttctggaat
     9001 tgttaaaaaa tattgaatta agcagtttga ccgttgaaca ggcccgcaaa ttgatggata
     9061 tgggtattga taggcagatc aaagaagatg taaaatcaac cagtgatttt aaaataacct
     9121 acaatgatat ttctctttct tgccgtctat atgagccatt taccactact gatgcactta
     9181 ttatatatta ccatggtggc ggatttatgt ttggtaatat tgaacttttt gaccatgtat
     9241 gcaggaaagc aaccaattct tcaggttgta aagtcatatc agttgaatac agattagctc
     9301 cagaacataa attccccgct gctgttgatg atgcatacca ttcatttaga tggattaaag
     9361 atcatgctaa aaactttgat attaatcctt caaaaattgc tatagcaggc gacagtgctg
     9421 gcgccaactt agccacagta acctgtcttc gatgtaaaga taccagcatg gatttaccca
     9481 aacttcaggt actcttttat ccagttgtgg cacctgatgt atgttcagaa tcagtacggc
     9541 aatttggaga tggatttttt ttaaccagag catcaatgaa atggttcaca cagctatatc
     9601 tttcatcacc tgctgatttt attaatcctt acatgttccc aatctttcat caaaatcctg
     9661 ctgggatccc agaagctatt attataacag ctgaacatga tcctttacgt gatcagggtg
     9721 aaatgtataa cctgttttta cgtgaagcag gaatacagtc aacatgtata caggcaaagg
     9781 ggatgataca tggattcttg aatttcagct acctgatacc atcggctgta agtattgcag
     9841 atatggtctg gtgtgttgtt ggcaaaaaac ttgttgttta aatgtattat tttatcctga
     9901 tatattctat ctcagcttgt gtctttgtgc tgcacacaag aatataaatg ccagaagatt
     9961 tcaagagatc atgttttttt gtattaatct ttcgcgccac aagttcccca tcaataaaca
    10021 aggaaagcgt tttgccttca ctacgcaatt catactcatg ccattgagtc gttgtatacg
    10081 ggattttgta tccgcacaca tcaacctctt caggcaacaa tgttatcacc gcagcaagcc
    10141 ctccgtagcc aacaactcca gcaagaataa cattcccact aagcaagcgc ccttttacct
    10201 tacacaccat attctgaaag ctaccagttt ttttgtaaag accacccttt tcacctgaat
    10261 cgagcttaac aatatgtata tctttaagtt tggtaaaact tatagcttcc tcaaagccag
    10321 gatacatatg ttgccagcct tttggcgtat gcgaatcaat ccattcgcta aaatgttcat
    10381 caatagtaaa accttcatcc tctgagacca aattttttgt cataggctca atgcgctcta
    10441 aaaacttgcc aaacacccat cccctgctgc cacttacgga aactaccctg taccagtagt
    10501 cacgcacatc gccaatctgt actgaatcca ttgaacgctc taattgaata acaaattcac
    10561 ctttattaag ctttccggta atctgcccat gagtaccagc tgaatcacgt agattgacat
    10621 tttcaccttt gcatctgaac aaccggtaat actctgcact tgtgtgatac ttattgcaga
    10681 agttctgaat catttcagta agcttttcag catgcccttt taattcaatt tttgggtttc
    10741 ccagcgcttt taataccata tttacaagcg atgacaggta cgtagtttca ggatagcgtt
    10801 tatagaactc aataactgga ttgatagtac ccggatgttt gctcataata aattgcagca
    10861 tatcaaagtc tattgattct atgtagcgac tgccaggata caatgacaca aattcgtcat
    10921 ataacgtgcc acccatggat atataacact ccggatcagt aacaagaata agatccgcct
    10981 ctgtctcttt gtttatttct tctaatttag ctttgagctt agattcaatt tttttgtttt
    11041 ttgttttttg tatttctttg cactcatcag catagtcctc attcaattca ttaataaatc
    11101 ctaaaagcgc ttttaaacgg taataatata tgcgttcacc atcttcataa cctgaagttt
    11161 ttgcaagtgt attaagcacc tttaacgcct tcttataatt cccgctgtca atgtactcaa
    11221 tagcattgtc aatggttgcc ggtgccatag ctctatatat aacgacgcca gctataatca
    11281 caccaataac tacaagagct ataaattttt ttgacatata ttcctcacca tggataaaca
    11341 ttgttcacaa tcattgctaa aagttcatgt gcatgttcaa caaatatacc agtatccctt
    11401 gcatctactt cattaaagcc tttacaatta aagcgatagt tcatgtcagg ggcatatata
    11461 acaaaaggaa caggatcgct tgaatgtgta cgcaagctaa gcggtgttgg atgatcaggc
    11521 atcaccagca ctgtgaaatt gccgtagcga ttgagcttat ttacaacagg acccacaact
    11581 ttagcatcaa aatcctctat cgctttaagt ttatgttcta aattaccttc atgccccgat
    11641 tcgtcaggtg cttcaacatg caaaaacaca aaatttgcat tcttcattgc atcaatacaa
    11701 gcctgtgctt taccgctgta gtttgtatca atatagcctg ttgcaccttc aacatgaatt
    11761 ggggtaagtc ccgctgccct tccaatacca tgtattaagt caaccgctgc aatagtatgt
    11821 ccgtgtaatc caaaccgtgt tgagagtggc tccatctttg gcctgaagcc acctccccac
    11881 agccataacc ccgttggatt gcccttaaac tttagttttg catctgcaat ttttttacta
    11941 tgagctatcg cccctgctgc tttttgcatg atgtcattta gcaattcact gtttgcacct
    12001 ttaggcaagt attcatgaat tttttttccc tgtatatcat ggggtggcgt ggtaattggt
    12061 agttcacctt taaaattacg caccaccata atattgcgat atgatacacc tgcataaaat
    12121 tcaacgccat ccctgtgtat tgtatttttt atttcatcaa tgacaatctt tgaaaattct
    12181 gaatcaatgt gacctgcagc aaaatcgttc ataatatcat gttcgatagt taccatatta
    12241 caccggaatg caacatcatc aggtcctaaa ttaatgccca tattgagtgc ctcaagcggt
    12301 gctctgccgg tataacaggt agcagggtca tagccaaaca ccgaaaggtt tgcaacatcg
    12361 cttcccggtg gcatgcctga aggtaccgtg cgagcaagac cgcatattcc attttttgcc
    12421 agataatcca tgtgatcggt tttagcatac tgtaatggcg tgttacctcc taactcttcg
    12481 attgggtaat cagccatgcc atcgccaata agtattgcaa tttttcgctc actcataaag
    12541 taattccacc atatctcctg ttgttacagc taaacgtttg cttgcatctt tttgattcat
    12601 acaaatttca ataaatccgc tactaccttt taatatacca attgcctcta atggcacatc
    12661 agcatacgtt ttacaatggt ataaagtata gcgcttatta ttaacgatta ttgcatttaa
    12721 ttgtaaagaa tactgttcat ttggcaatga taaaatacaa ttgccaaacc tgtcaatgtg
    12781 cactaccctt gtatacacag tatccacttt gatgtcatac catggaaacg gatgttgtac
    12841 ataatttgtg cactccttgc aatggatagc ctctataccc tgcatggcta atcgggaagc
    12901 taacattgca aaaatgtctc gtccatgaaa tgttgatgaa gcatggacaa actcctgtgt
    12961 atccactgac cacactttta tggtattctg attaagacat cctgctatac cattatctgg
    13021 cataaaaaaa taatacctgt catcatgtat cagtaaaata tttcgttgtg tacccactcc
    13081 aggatctaca actaccaaaa agatagtatt atcagggaaa tacctgtacg atgttgtaag
    13141 gatatattga gcatgttcta ttgaataagg gactatatgg tggcatatat ccactataga
    13201 tccggtatat ccttgtgaat aaatcactcc tttaacaaca ccaacatagg gatcatttat
    13261 tccaaaatct gtaataaggg ctatcatgac aaacctttgc tttttgttac tatttttaaa
    13321 ttatatcaaa ctattgtcaa attgaaaaaa ctcagtttat ttcatcatca aaaatgaaat
    13381 tgatgattat gttattttca atcctacctt tttaagaaag gcaatacatc tcattaacag
    13441 gaaacttcat gttgattatt ttagataaaa aagacttgca attacgctat attcttagta
    13501 tacgttaaga gaacagtgaa tttataaaca tttaactcac tttaaatatc atggaaagta
    13561 cattagaaaa aatccaattc aggctaaacc agagcaggga aaaaatcaaa ctggcttcca
    13621 ttttccttga aaaaaagaaa tattgtgatt ccgttttagc ttcttaccgg gcaatttttt
    13681 atgctat
//
LOCUS       DAORYL010000079        13649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_3548_length_13649_cov_11.416581, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000079 DAORYL010000000
VERSION     DAORYL010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13649
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3548_length_13649_cov_11.416581"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2102)
                     /locus_tag="PLV81_12300"
     CDS             complement(<1..2102)
                     /locus_tag="PLV81_12300"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HOM10924.1"
                     /translation="MDKIFKRDFKYREIPYNYTSFSDREIILKYFDAQTWDLVQALRA
                     KRRTGRSAKLLFENIGDIFIIDRNPYIQYDIVENPAKLKQLYKRHQRRLATVKEGANG
                     DQQVLEFIAKIEKLDEAFFRKLKETKKLQEKIFNRLKHITAAGNIHFSPFHRASHVTD
                     ATDWRVEYPVVVVYPDSVHEIQELVKAAKKLNLVIIARGGGTGLTGGAIPLLPNTMII
                     NTEKLNNIGKIENTTINGKTVPTITVEAGAVTEDVMEYCEHQNYIFATDPTSAWACTI
                     GGNIAENAGGKKCVMWGTCIDNILSFDIVDHNGDIITVRRVDHPYRKILPGDEVLFTV
                     EKNKKLLKTITLSGLDIRKKGLGKDITNKALGGLPGLQKEGCDGIIVNATFVLYRPFK
                     YTRSVCFEFFGNNMINASKAIVEIVKTFEDDPVVFLTALEHFDEQYVKAIQYKNKSSR
                     TEIPKAVLVVDIESDDEAKLEKATEDLVAKVKQFNTEGIIAKDTETREKFWQDRKNLS
                     AIAKHTNAFKLNEDIVIPLEKLQDFSDFIEKLNVKKELENNIQIIRALIDYLQERVKT
                     EEDDVCIERCHAGMGQLLSMQSRYTDILNNLDTPAKDYFKYDSEYALRLNTVFQLIQN
                     NELTMDFEKEVDEPLQKLFYGYDDILEKILQVKEATGKRRIVVATHMHAGDGNVHVNI
                     PVHSNDYLMMRDADETAAT"
     gene            2277..2876
                     /gene="yedF"
                     /locus_tag="PLV81_12305"
     CDS             2277..2876
                     /gene="yedF"
                     /locus_tag="PLV81_12305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HOM10925.1"
                     /translation="MKEVDARGLECPKPVLLTKETIEKDNPDNLTVLVSNDTAVGNVT
                     RFAENCGYSVTTTQQGNYFILHLSKGTQAQSQPVSQPRTMESVNENIVFFITSNEFGQ
                     GHQELGQLLIKLFINTINEANMLPATMIFVNSGIFLTTEGSPVLPALQEIAQKGVQII
                     NCGTCLDFYKRKDQLKVGSISNMYTITETLMKATKVIRF"
     gene            complement(2923..3966)
                     /gene="ruvB"
                     /locus_tag="PLV81_12310"
     CDS             complement(2923..3966)
                     /gene="ruvB"
                     /locus_tag="PLV81_12310"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOM10926.1"
                     /translation="MNKKEKNNNTPQSLISPLSLEEEDFEKKLRPQFLNEFIGQKQLT
                     ENLRIFIESAKLRKKPLDHVLLAGPPGLGKTTLAFIVANELGVNIKATSAPAIEKPGD
                     MASMLTNLEPMDVLFIDEIHRLSPTIEELLYGAMEDGELDIIVGQGVGAKSIKLQLAP
                     FTLIGATTRTGLLTSALRDRFGIPLRLDYYEIADLETIAKRTANIIGCIIDDMAAQEI
                     ARRSRRTPRIVNRLVKRVLDFAIVMKKGSIDLDIARYALDRLHIDSLGLDDMDRKILT
                     TIIDKYDGGPVGIKTIAISIGEEITTLEDFYEPYLVQIGMLKRTPRGRVAARLAYEHL
                     QLPYKGMDESSLF"
     gene            complement(3941..4417)
                     /locus_tag="PLV81_12315"
     CDS             complement(3941..4417)
                     /locus_tag="PLV81_12315"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOM10927.1"
                     /translation="MVLTDYLNKKTILINPSVKDRWELIESMLDVAIKNKAVKSEYRE
                     TIKKTLFEREKSMSTGIGKGVAIPHCSCPYVDDVVMILALSKKGINFDSIDNLPVHIA
                     ILLIVPKDKISQHIKTLANIAKLMSDDALREALSNAKDAQDIIKILKEYEQKGKKQ"
     gene            complement(4564..5217)
                     /locus_tag="PLV81_12320"
     CDS             complement(4564..5217)
                     /locus_tag="PLV81_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOM10928.1"
                     /translation="MPVNRSELVSFYSHMIGVVGAIIGLVMLIVTTNKPLIQILSVIY
                     GISVIMLFTASTLYHGLKQQENEDSLWRRLDHMAIFIMIAGTYTPVCYIYLDYKTALY
                     IIIAQWLLVVLGIIFKIYFFNAPRVIYTAIYLAMGWMAVLVIKDIVRIMDTVSLWYLF
                     LGGVAFTIGALFYIVKWPQKHHGYVGFHEIFHGFILAGAFFHYMMVYHSIQYIQNIH"
     gene            5424..5885
                     /locus_tag="PLV81_12325"
     CDS             5424..5885
                     /locus_tag="PLV81_12325"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOM10929.1"
                     /translation="MNAKTVKESTITIVQQMTHQDANLAGNVHGGTIMKLIDNTGGIV
                     ASRHAGCLVVTASIDRLDFHSPVYVGDLLRLKASVNYVGTTSMEVGVRVEAENFITGE
                     VRHTASAYLTFVALDEHGKPKNVPPLQPETEDEKRRYQEAEIRRKKRLGNK"
     gene            5913..6227
                     /gene="cutA"
                     /locus_tag="PLV81_12330"
     CDS             5913..6227
                     /gene="cutA"
                     /locus_tag="PLV81_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOM10930.1"
                     /translation="MEYCVIFCTVPTKEVGLEIANTLVSKQLAACVNIVPGLTSIYSW
                     KGEICNDQELLLIIKTRGNLYTNIEEAIKKIHPYEVAEIISLPITSGSYEYLQWIVNV
                     TK"
     gene            complement(6358..6600)
                     /locus_tag="PLV81_12335"
     CDS             complement(6358..6600)
                     /locus_tag="PLV81_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10931.1"
                     /translation="MNNDTMVDLTTIRELRELEASLRNEIQTAKGEIIRDLQKKEEYL
                     THLIRVLIAKIDALEQEKKAVFQEKPKTNTRRLFRI"
     gene            6912..7358
                     /locus_tag="PLV81_12340"
     CDS             6912..7358
                     /locus_tag="PLV81_12340"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10932.1"
                     /translation="MKIIKYLTLILFIFSLSCASAPKNCKEGDCNNGVGTTIHENGSY
                     KGNFKNSTREGLGEYTFNNGDTYRGNYKNNLRNGEGEYKWKNGDSYRGNWVDGKRNGF
                     GIYTWSDGTRYEGEWKDDARHGKGKLILKDGTTFDGMWENDAYIRK"
     gene            7394..7726
                     /locus_tag="PLV81_12345"
     CDS             7394..7726
                     /locus_tag="PLV81_12345"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOM10933.1"
                     /translation="MNKRNVLMVCCIALLIPAFIFAQGVVKVKKTSAAPKQGTVTFDH
                     QIHKSKGITDCKTCHHTGNTAKKCGDSGCHWETQNKGYNAIHSKCLGCHRTKGGKAPT
                     LCTQCHKR"
     gene            7771..8826
                     /locus_tag="PLV81_12350"
     CDS             7771..8826
                     /locus_tag="PLV81_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10934.1"
                     /translation="MPGLITHQRIFFESVKSLKKQKHPNYATHLLDIQFTNPRFTSSA
                     MAGLLVPNLFDYIPGKRHIFFGNSIKHLLHSDGGIPIIHYMFSTILKHSSHEPDEWLY
                     YQKGFFYGYVSHWVVDALFHPFVFYWSGFPTFKDNRISLYRNQHLLFEYTIDQYMAYF
                     FNSEPFDYNLKAMLPVKGSVQYKAIKDLISGAIAHTNTSLKINIPLFPYTPLDTFDIA
                     VKCVPFVYMIKNNRSPQFVRILRFLEKRFYSDFLVRYPDGRRINRHILNLHRERWFIP
                     TGEPGLHYESVDDLFRIARDKTVFIWKQLEELFSKQEHITVQQLNTVLEYSNAYTGKK
                     GFSIDKMTLQNPIRLRN"
     gene            complement(8830..9912)
                     /locus_tag="PLV81_12355"
     CDS             complement(8830..9912)
                     /locus_tag="PLV81_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10935.1"
                     /translation="MGSFTVKKKGLLLLLLLLWPLSILGYEVNISSVRPNLMNIRTTN
                     GKLYAYIVLLFNENPHFVNLIQADSIPVRLMQTQGFNVMQYDAYIRTLQDAPLKGLAQ
                     QILQVYKEPDKITDTELEQLTQKLENRNIILRFSKTKDGNGSRYLLDYCIFGKRKPVT
                     INHPLFNVKEKIFNIQPLIYYDEFSTSNSTFYFDMIYINPDEVYNDYIISKRIIEGKS
                     CENMFFVGAHVTEDIKYCIKKAFASKETIFDEIWRMFEIHELTHKILNNYYYFFDQVT
                     GEELALSSTIYYNTYLGLAILYSYLDYNTINPHRIAATNFIKFLSLYTANSEYIEKPS
                     LIKQIPLEDIKKIAKLHFETTIKNLK"
     gene            10142..11056
                     /locus_tag="PLV81_12360"
     CDS             10142..11056
                     /locus_tag="PLV81_12360"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOM10936.1"
                     /translation="MKLLAQGTINDYCQVIGHIGYPAYIVKGHHKIMMIDAGINLLAP
                     KYYTDLSTILGDPQKLNYAAITHSHYDHLGAIPYLLKKIPGLTIVGAERIQQLLAKQS
                     VIDFMTHLSNIQRPLFADITGQEDVSLYSFNITLPVKDGDTIDLGGLTCIVYEVPGHT
                     RDSLAYYFPQIKMLCPGEAVGVPQGMDEQDVQVEFLSSYEEYFSSLEKMASLEVKILC
                     MAHGFVYTDDDVASFFRASLKATAQYKNLLLEYLDKVNGDIAQAIELITKEEYDKKGT
                     IHQERNAYISNLSAQVKLVAKDYQYIKG"
     gene            complement(11046..11255)
                     /locus_tag="PLV81_12365"
     CDS             complement(11046..11255)
                     /locus_tag="PLV81_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10937.1"
                     /translation="MATTENKKKKSKKKSTLFDKVFTLIIQYKETSLFRWVYNHPGLA
                     MSLVALWGIIIVVMFMWFVISLATP"
     gene            complement(11354..12844)
                     /locus_tag="PLV81_12370"
     CDS             complement(11354..12844)
                     /locus_tag="PLV81_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOM10938.1"
                     /translation="MPNQMPENLKNIKDILDIDEELHKVSPEAETVGGKAGIRSRRGD
                     INFQLKPEELESYLNKYIVGQEEAIEIIATKICTHFNRMHLEQELPEDEKIVGNIKSN
                     MLLIGPTGVGKTYIIKLIAKKIGVPFVKADATKFSETGYVGGDVEDLVRELVHEADGD
                     ISKAEYGIIYLDEIDKIASSGNVYGPDVSRAGVQRNLLKLMEEADVDLKTPHDLASQV
                     EAAMEAQRTGKVTRKKINTKNILFIVSGAFGGLEDIINKRLNKQAIGFDKSQVRREDR
                     QSVLKMVKTEDLIEYGFESEFVGRLPVTVVLNDLDEEGLYKILKNKYSTVVLGKKLDF
                     RAYGIDLEFTDEALRMLASKAYKERTGARGLLSVFEKTLIKFEKTLPSTDIKKLTVDR
                     EVVEHPEEVLKRILLSDNIRKFQKEFLIKHGIVMEFEDQALQIIQEKARAAGMSIKQY
                     CDDLFHDYPYGIKLMNLERFTITKAAVEDPQGYIDEYIRNYYSQKK"
BASE COUNT         4324 a         2657 c         2263 g         4405 t
ORIGIN      
        1 gttgctgcag tttcatcagc atcacgcatc atgagataat catttgaatg tacgggaata
       61 ttgacatgca cattaccatc acccgcgtgc atatgggtag caacaacaat gcggcgcttc
      121 cctgtagctt cttttacctg aagtatcttt tccagtatgt catcataacc gtaaaaaagt
      181 ttttgtagtg gttcatccac ttccttttca aagtccatag tcagttcatt attctgtatg
      241 agctgaaaaa cagtattcag cctgagtgca tattccgagt catacttgaa ataatcctta
      301 gctggagtat caagattatt aagaatatca gtatagcgcg actgcatcga cagtaactgt
      361 cccattcctg cgtggcagcg ttcaatacat acgtcatctt cttcggtctt gactcgttcc
      421 tggagataat caataagtgc gcgtataatc tgtatattat tctccagttc ttttttaaca
      481 tttaattttt caataaaatc cgaaaaatcc tgcaactttt ccagaggaat aacaatgtct
      541 tcattcagct taaacgcatt ggtatgtttt gcaatagcac ttaaattttt tctgtcctgc
      601 cagaattttt ccctggtttc agtatctttt gcaataatcc cttcagtatt gaattgctta
      661 actttcgcta ccagatcctc tgtagccttt tctaactttg cttcatcatc gctttcaata
      721 tcaactacca gtactgcttt aggaatttca gtacgagaag atttattctt atactgaatt
      781 gctttcacat actgttcatc aaaatgttct aaagctgtta aaaacacaac cggatcatcc
      841 tcaaaagttt ttactatttc aacgatagct tttgaggcat tgatcatgtt attgccaaaa
      901 aactcaaaac atacagaacg ggtgtattta aacggcctgt acagaacaaa cgttgcatta
      961 acaataatgc catcacaacc ttctttctgc agtcctggta atccacccag tgctttattg
     1021 gtgatatcct tccccaggcc ttttttacga atatcaaggc ccgataaggt tatggttttt
     1081 agtaattttt tatttttttc tacagtaaaa agaacctcgt caccaggcaa tatcttccgg
     1141 tagggatgat ctaccctgcg tacagttatg atatcaccat tgtgatctac aatatcaaat
     1201 gaaagaatat tatcaatgca ggtaccccac atgacgcact tcttaccacc tgcattttct
     1261 gctatattac caccaatagt gcatgcccat gcggatgtag ggtcagtagc aaatatataa
     1321 ttctgatgtt cgcaatattc cattacatct tcggttactg caccagcctc aaccgtaatg
     1381 gtgggaacag ttttaccatt aatagtcgtg ttttcaattt tcccaatatt gttcaatttt
     1441 tcagtattga ttatcatagt gttaggcaaa agaggaattg caccaccagt aagtcctgta
     1501 ccaccaccac gtgcaataat aacaagattc agcttttttg cagccttcac cagttcctgt
     1561 atttcatgca ctgaatcagg atacaccaca acaacaggat actccacacg ccagtctgtt
     1621 gcatcagtta cgtgagatgc acgatggaag gggctaaaat gaatattgcc agcagcagtg
     1681 atatgtttta atctattaaa aatcttttcc tgcagcttct ttgtttcttt cagctttcta
     1741 aaaaaagctt catcaagctt ttcaattttt gctataaatt ccaggacctg ctgatcacca
     1801 ttagcacctt cttttacggt tgcaagcctt ctttgatgtc gtttatacag ttgtttcagc
     1861 ttggcagggt tttccacaat atcatattga atatagggat ttctgtctat aatgaaaata
     1921 tcgccaatat tctcaaagag aagctttgca ctcctgcctg ttcgccgctt tgctctgaga
     1981 gcctgcacca gatcccaggt ctgagcatca aaatacttta agattatctc cctatcagaa
     2041 aatgatgtat agttataggg tatttccctg tatttaaaat ctcgtttaaa aattttatcc
     2101 attcagaacc ctgttaaaaa attgttttaa aaatttactc aagtataatt attataccac
     2161 aagggtttaa aatagcaatg tgttacatca agtattaata ctacaaaata acgtaataca
     2221 ttgtacgata gttactggtc aatatcttaa ttaaaaattt tttaagggag atagttatga
     2281 aagaggtgga tgcgcgaggg cttgaatgtc caaaaccagt acttttaacc aaggaaacaa
     2341 tagaaaagga taatcctgat aatctgacgg tactggtaag caatgacacc gcggtgggca
     2401 atgtaactcg ctttgcagaa aattgtgggt attctgtcac aacgacacaa cagggcaatt
     2461 actttatcct ccatttaagt aaaggcacac aagcacaatc acaacctgtg tcacaaccca
     2521 gaacaatgga gtctgttaat gaaaacattg ttttctttat tacatccaac gaatttggtc
     2581 agggacacca ggaactgggc cagcttttga taaaattatt catcaacaca attaatgagg
     2641 ccaatatgct gccagctacc atgatatttg ttaattccgg gatatttcta accactgaag
     2701 gatcaccggt actgcccgca ttgcaggaaa ttgcacaaaa aggtgtgcag attattaact
     2761 gtggcacatg ccttgatttt tataagcgca aagatcaatt gaaagttggc agtatatcca
     2821 acatgtacac catcactgaa accttaatga aagctacaaa agtaatacgt ttctgattgt
     2881 ggatttgtaa tagaaggatg gtatgtggta ttgcctcaat tgctaaaaca ggcttgattc
     2941 atccatgcct ttgtagggaa gctgcagatg ttcatatgcc agacgtgctg ctaccctgcc
     3001 gcggggagtt cgcttcagca tgccaatctg taccaggtat ggttcgtaaa aatcttccag
     3061 cgtggttatc tcttcaccta ttgagatggc aattgtcttt atgccaacag gtccgccatc
     3121 gtatttatca atgatggttg tcagtatctt tcggtccatg tcatcaagcc ctaaagaatc
     3181 aatgtgaagc ctgtccaaag catatcgtgc gatgtcaagg tcaattgacc cttttttcat
     3241 aactatcgca aaatcaagta cacgttttac aagcctgtta actattcggg gagtacggcg
     3301 agaacgccgt gctatctcct gtgcagccat atcatcaata atgcacccaa taatatttgc
     3361 agtccgctta gctatcgtct ctaaatccgc aatctcataa taatccagcc tgagagggat
     3421 gccaaatcgg tcacggagtg ctgacgtcaa taaacctgta cgagtagttg cgcctattag
     3481 ggtaaagggt gcaagttgca gcttaatact ttttgcacca acaccctggc ccacaatgat
     3541 atcaagctca ccatcttcca tggctccata taataattcc tcaatggtgg gtgaaagcct
     3601 gtggatctca tcaataaaca ggacatccat tggctccaga ttggttaaca ttgaagccat
     3661 atcaccaggt ttttcaattg ctggtgctga agttgccttg atattgacac caagctcatt
     3721 tgcaacaatg aaagcaagtg tggttttccc aagccctggc ggtccagcaa gcagtacatg
     3781 atccaggggt tttttgcgca gttttgctga ctcaataaat atacgcagat tttcagtaag
     3841 ttgcttttgc cctatgaatt cattgaggaa ttgagggcgt aatttttttt caaaatcctc
     3901 ttcttctaaa gatagcggag aaataaggct ttgaggtgtg ttattgtttt tttccttttt
     3961 gttcatactc ttttaaaatc tttataatat cctgagcatc ctttgcattg gatagggctt
     4021 cacgcaaggc atcatcactc ataagttttg ctatatttgc cagtgtcttg atatgctggg
     4081 agattttgtc tttggggaca attaaaagaa tagctatatg cactggcaaa ttatcaattg
     4141 aatcaaagtt tatacctttt ttactcaatg ccagtatcat caccacatca tcaacatacg
     4201 ggcaactgca atgaggtatg gccactccct tccctatacc ggtactcatt gatttttcac
     4261 gttcaaataa cgtttttttt atggtttcac gatattcgga ttttacagct ttatttttaa
     4321 ttgccacatc cagcatcgat tcaatcaatt cccaccgatc ttttaccgac ggattaataa
     4381 gtattgtctt tttatttaaa taatcagtta aaaccatata tacaatcaac cccaaaaata
     4441 aatcaacctc tataattaca actaaaatac tacccgtata agaatttcgt caagtgtaat
     4501 ataatttttt tgatagttac aacgtatatg ggatctttaa aaagtgaggg aataattttt
     4561 gttttaatga atattttgta tgtactgtat actatggtat accatcatat aatggaaaaa
     4621 agctcctgcc agaataaaac catgaaaaat ttcatgaaaa ccaacatatc catgatgctt
     4681 ttgcggccac tttacgatat agaacagtgc gccaatggta aacgcaacac cacccagaaa
     4741 aagataccac agggatacag tatccataat cctgacaata tccttgataa ccagtacagc
     4801 catccatccc atggccaaat atattgcagt atatataaca cgcggcgcat tgaaaaaata
     4861 tatcttgaag ataataccca ataccaccag taaccactga gcaataataa tatacagagc
     4921 ggttttataa tctaaataga tgtaacatac aggagtatac gttcccgcaa tcatgatgaa
     4981 gatagccata tggtccaatc ttctccatag tgagtcttca ttttcctgtt gcttcagccc
     5041 atgatacagg gtgctggcag taaacagcat aatgactgaa ataccatata taaccgatag
     5101 tatctgtata aggggcttat tagtagttac aattaacatt accaatccaa taatagcacc
     5161 aacaactcct atcatatgtg aatagaatga tacaagttct gatctgttaa ctggcatact
     5221 atacctttta tattcattga ttaaatagat agaaacagta aatactttat tgccataagt
     5281 gatagtacca tatactcaca ttttttaatg cattcattta tggacaattg atttgttttt
     5341 ttggttgcaa aaatttatgt ttttcatata taaaaatata ctaattcata tattttttat
     5401 tccattgaaa gtacatcaat tgtatgaatg caaaaaccgt caaagaaagc actataacaa
     5461 tagtacagca gatgacacat caggatgcca atctggctgg aaatgtccat ggtgggacta
     5521 tcatgaagct gattgacaac accggaggca ttgttgcttc ccggcatgca ggctgtcttg
     5581 ttgtgacagc atcaattgac agattggatt ttcacagtcc tgtttatgtt ggggatctgt
     5641 tacggttaaa agcaagtgtt aactatgttg gaactacctc catggaagtt ggtgtacgcg
     5701 ttgaggctga aaattttatc actggagaag tgcgacatac tgcttcagca taccttactt
     5761 ttgttgccct ggatgaacat ggtaagccta aaaatgtacc cccgttacag ccagaaaccg
     5821 aagatgaaaa acgccgatat caggaagccg aaattagaag aaaaaagcgg ttgggaaata
     5881 aataaagtaa attttgtaac aggagtttat gtatggaata ctgcgttatt ttttgtactg
     5941 tccccaccaa agaagtaggt ttggaaatag ccaataccct tgtttcaaaa caattagctg
     6001 cctgtgtcaa tattgttcca ggtcttacgt ctatttattc atggaaaggc gaaatatgca
     6061 atgaccagga attacttctt atcatcaaaa ccaggggtaa tctgtatact aatattgaag
     6121 aagcaataaa aaagatacat ccctatgaag tggctgaaat tatttcttta ccaattacaa
     6181 gtgggtcata tgaatacttg cagtggatag tgaatgttac aaaataaaaa aattccgtac
     6241 ttgtggtacg gaattttata tagaaacgca tggtgaggtc atcacctatg ctgtcatata
     6301 ctatttatat cacaggccgt gattttttca aaaaatttta tttatatatt ctaattatta
     6361 tatacggaac agccttctgg tgtttgtttt tggtttttcc tgaaatactg ccttcttttc
     6421 ctgctccagt gcatcaattt tggcaattaa tacacgaata agatgagtaa ggtattcttc
     6481 cttcttctgt aaatcgcgta tgatttcacc tttcgctgtc tgtatctcat tacgcaaaga
     6541 tgcttctaac tcgcgcaatt ccctgattgt tgttaaatct accattgtat cattattcat
     6601 cggtctctcc attgtagata aataatggtg cccttttcac tatactgtat atagcagcaa
     6661 ctttactttt gcctttccta taactacaca gctttacatc caatactatt gaatatagca
     6721 ttcaattgta aaagttcatt aatattttat atacaaatta gtatttattt catcctgcaa
     6781 tctactcgta tattgaacta tcgcccatac ccattttttt aaaaaactat ttgcgtaata
     6841 ttaattcatt ttatacaatg cctacagtaa aaatactcta tagagttatc tcacagtaag
     6901 ggaggagcag catgaaaata ataaaatatc ttacacttat actattcata ttttctttaa
     6961 gctgtgcttc agcaccaaaa aactgcaaag agggtgattg caacaatgga gttggcacaa
     7021 ccattcatga gaatggttca tataagggaa attttaaaaa cagtacccgt gaaggtcttg
     7081 gcgaatatac attcaacaat ggcgatacct atcggggcaa ttataaaaac aacctgcgta
     7141 acggcgaagg tgaatacaaa tggaaaaatg gcgatagtta tcgaggcaac tgggttgatg
     7201 gtaaaagaaa tggttttggt atttatacct ggtctgacgg cacacgatat gaaggggaat
     7261 ggaaggatga tgctcgccat ggtaaaggca aacttatact caaagatggg accacctttg
     7321 atggcatgtg ggaaaatgat gcgtatatcc gaaaataatt ttaatgataa tctaaacatt
     7381 tttaggaggt tatatgaata agaggaatgt gcttatggtt tgttgcattg cactcctgat
     7441 tccagcattt atctttgcac agggtgtggt gaaagtaaaa aaaaccagtg cagcacctaa
     7501 acagggaact gttacatttg atcaccagat acataaaagc aaaggaatta ccgattgcaa
     7561 aacatgccat cataccggca atactgcaaa aaaatgcggt gatagtggat gccactggga
     7621 aacacagaat aaaggatata atgccattca cagcaaatgt ttaggatgcc acaggacaaa
     7681 aggtggcaaa gcccctacac tatgtactca atgccataaa cgataacaca atatctgggg
     7741 gattgttaaa tcccccagac atgtattcat atgcctggat taataaccca tcaaagaatt
     7801 ttttttgaat ctgttaagtc attaaaaaag caaaagcatc ccaattatgc aacacatctt
     7861 ctggatatac aatttaccaa tccccgtttt acttcctctg ccatggcagg actgcttgtc
     7921 ccgaaccttt ttgattatat cccgggaaaa agacatattt tttttgggaa ttctataaaa
     7981 cacctgttac attccgacgg tggtataccc atcattcatt acatgttttc taccattctc
     8041 aagcattcat cacatgaacc tgacgaatgg ctgtattatc aaaaaggttt tttctatgga
     8101 tatgtttcac actgggtggt cgatgcactg tttcatccat ttgtatttta ctggtcagga
     8161 ttcccaacat tcaaagacaa caggatctca ctctatagaa accagcatct tctctttgaa
     8221 tatactattg accagtacat ggcgtatttt tttaattctg agccatttga ctataatctg
     8281 aaggcaatgc tgcccgttaa aggttcagtt cagtataaag ccatcaagga tttaatttcg
     8341 ggagctatcg cccataccaa tacttccctg aaaataaata taccattgtt tccctacaca
     8401 ccgctggaca catttgacat tgcagtaaaa tgtgtgcctt ttgtgtacat gattaaaaat
     8461 aatcgcagcc ctcagtttgt tcgtattctg cgctttctag aaaaaaggtt ctacagcgat
     8521 tttctggtac gataccccga tgggaggaga attaaccgtc atatattaaa tcttcaccgt
     8581 gaacgatggt ttattcccac cggtgaaccg ggattgcatt atgaatcagt ggatgacctg
     8641 ttcagaattg caagggacaa aaccgtgttc atatggaaac agcttgaaga actgtttagt
     8701 aagcaagaac atattaccgt acaacaatta aatactgtgc tggaatattc caatgcctat
     8761 actggcaaaa aaggattttc aatagacaaa atgactttgc agaaccccat caggttacgc
     8821 aattaacggt tattttagat tttttatagt tgtttcaaaa tgaagttttg caatcttctt
     8881 tatatcttca agcgggattt gttttataag agatggcttt tcaatatatt cactattagc
     8941 agtatacagt gataaaaact taatgaaatt tgtagcagca atgcgatggg gattaatagt
     9001 gttgtaatcc aggtagctgt aaagtattgc caggcccaga tacgtattat aataaatggt
     9061 actgcttaat gccagttcct cacccgttac ctgatcaaaa aaataatagt aattattcaa
     9121 tattttatgc gtcaactcat gtatttcaaa catcctccag atttcgtcaa aaatagtttc
     9181 cttgctggca aaagcttttt ttatgcaata ctttatatct tcagtaacat gtgctccaac
     9241 aaaaaacata ttctcgcacg atttaccttc aattattctt tttgaaataa tgtaatcatt
     9301 atacacttca tcagggttta tgtatatcat gtcaaaatag aaggttgaat tgctggttga
     9361 aaattcatca taataaatta aaggctgaat attaaatatt ttttccttaa cattaaaaag
     9421 agggtgatta atggttaccg gttttctttt gccaaatatg caatagtcaa gaagatatct
     9481 ggatccattg ccatccttgg tttttgaaaa gcgcaaaata atattccggt tttctaattt
     9541 ctgtgtcagc tgttctaatt cagtatctgt tattttatcc ggctctttat aaacctgtag
     9601 aatctgctgt gccagacctt ttaagggtgc atcctgaagt gttctgatat acgcatcata
     9661 ctgcataaca ttaaaacctt gtgtctgcat tagccttaca gggatactgt cagcctgtat
     9721 taaatttaca aaatgcgggt tttcattaaa tagtaatact atataagcat acaacttccc
     9781 gttagtggtt ctaatattca tcaagtttgg acggacgctg gaaatgttta cttcataacc
     9841 caaaatgctc aacggccaca aaagcaataa gagcaacaac aatcctttct tttttactgt
     9901 aaaactaccc atattgtata taccttattt taaatcaatg tagctttgtt atcgttttta
     9961 tacaaaaaat aattaccatt aagcaatata ataaaaatat cttttcctac caaatattat
    10021 tgaaaaattt tatagttatt attttgtagt aatagttata tattattttt tttacaaatt
    10081 aaccggtttc aattcgtggg cgatagttct tagaataaac cttaccgtat aagaggttat
    10141 catgaaatta cttgcacagg gaacaattaa tgattattgc caggtcatag gacatatagg
    10201 ttatccggca tatattgtta aggggcatca caagattatg atgatcgatg caggcattaa
    10261 tcttttagca ccaaaatatt acactgacct ttcaaccatt cttggtgatc cgcaaaagct
    10321 caattatgca gcaattaccc attcccatta cgaccatctg ggagctattc catacctttt
    10381 aaaaaaaatt cccggattaa ccatagtagg tgctgaacgc attcagcaac tacttgcaaa
    10441 gcagtcagtt attgatttta tgacacactt aagcaacata cagagaccat tatttgcaga
    10501 tattacaggt caggaggatg tttcacttta cagttttaat ataaccttac ctgtaaaaga
    10561 tggtgatact atagatcttg gagggctaac ttgcatcgtg tatgaagtcc ccggccatac
    10621 tcgtgattct ttggcgtatt atttccccca aatcaaaatg ttatgtcctg gtgaggctgt
    10681 gggtgttcct cagggaatgg atgagcagga tgttcaggta gaatttttat cttcatatga
    10741 agagtatttt tcttccctgg aaaaaatggc ttcccttgaa gtaaagattc tttgtatggc
    10801 tcatggtttt gtatataccg atgatgatgt agcatcattt tttagggcat cacttaaagc
    10861 aaccgcgcaa tacaaaaatt tactattaga ataccttgac aaagttaatg gagatattgc
    10921 acaggcaatt gagttaataa ctaaggaaga atatgacaaa aaaggaacaa tccatcaaga
    10981 aaggaatgct tatatatcaa acctcagtgc gcaggtaaaa ttagttgcta aagattatca
    11041 gtacatcaag gggtagccag tgaaataaca aaccacatga acataacaac aataatgata
    11101 ccccatagtg ccacaaggct catagcaaga ccgggatgat tatacaccca tctaaaaaga
    11161 gatgtttctt tatattgaat aatgagtgta aaaaccttat caaaaagagt agactttttc
    11221 tttgattttt tctttttatt ttcagttgta gccattgttc cctcgcaata tcataagccc
    11281 accagccgtt ataaagatag catatatgaa cattttaata aatgtcaact aataaaatct
    11341 ataatatata aacctatttt ttctggctat aataatttcg gatatattca tcaatatacc
    11401 cttgagggtc ttcaactgca gctttcgtaa tagtaaatct ttcaagattc attaatttaa
    11461 tcccataagg ataatcatgg aataaatcat cacaatactg ttttatactc atccctgctg
    11521 cacgggcctt ttcctgtata atctgtaatg cttgatcctc gaattccatg actatgccat
    11581 gctttattaa aaattccttc tggaatttac gaatgttatc actcaacagt attcgtttta
    11641 atacttcttc aggatgttca accacttctc tgtcaacagt aagctttttt atatcagttg
    11701 atggaagtgt tttttcaaat ttaatcagcg ttttttcaaa tacactcaac aggcctcggg
    11761 ctcctgtacg ctctttataa gcttttgatg ccagcatacg taatgcttca tcggtaaatt
    11821 caaggtctat gccataagct ctgaaatcta actttttgcc cagtacaact gtactgtatt
    11881 tattcttcaa aatcttatac aaaccttcct cgtccaaatc attcagcaca accgtaaccg
    11941 gcaaacgccc aacaaattcc gattcaaaac catattcaat aaggtcctcg gtttttacca
    12001 ttttaagtac actttgcctg tcttcacgcc tcacttgtga tttatcaaag ccaatggctt
    12061 gcttatttaa gcgtttatta ataatatctt ccagaccacc aaatgcacca gaaacaataa
    12121 acagtatatt ctttgtatta atctttttac gagttacctt tcccgtgcgc tgagcctcca
    12181 tcgctgcttc aacctgtgaa gccaaatcgt ggggtgtttt taggtcaaca tctgcttctt
    12241 ccataagttt taagaggttg cgctgaactc ccgcacgaga tacatccggc ccatatacat
    12301 tgccacttga agctatttta tcaatctcat ctaagtagat aattccatat tcggctttag
    12361 aaatatcgcc atcagcttcg tgaactaatt cacgtaccaa atcctcaaca tcaccaccaa
    12421 cataccccgt ttcactaaat tttgttgcat cagcctttac aaaaggaaca ccaatctttt
    12481 ttgctatcaa tttgattata tatgttttcc ccactcctgt gggaccaata agtagcatat
    12541 tacttttaat attgccaact atcttctcat cttcaggtaa ttcctgttct aagtgcatac
    12601 ggttaaaatg ggtgcatatt tttgtagcaa taatctcaat tgcctcttcc tgaccaacaa
    12661 tgtatttatt tagatagctt tccagttcct caggttttaa ctgaaaatta atatcacctc
    12721 gtctggacct tatgccagcc ttaccaccaa cagtctcagc ttcaggcgat actttatgca
    12781 attcctcatc tatatccaat atatctttaa tattctttaa attctcaggc atctgatttg
    12841 gcattggttt acctcgttta cagcatagga tttttgttac ttcaattata gcatgaaatt
    12901 ggccttcatt atattaatat aacaaaaaaa tctataaatg tggtaattat tttgcttata
    12961 gcattataaa gaatgtttat gtcttataat aaacattctt ttatgacgta atgtcgcttt
    13021 caattcacat attttgtaca gtgctatata taaccatcat ttatgatata tcttcaaaac
    13081 aattagaaat attgaatata tatttaaaaa acaaaagatt gagtatataa ttaatttact
    13141 acaatataat caagaactgc aattattggg gaataaaagg aatttttaaa acaagaaaca
    13201 ttctcaccaa tcaagaaaat actaactata gtacacagtg aatgcagcta tacacagatt
    13261 ataaaaaatc cctcctggag aagcgactca taaatattat ttccagagat gggaagctga
    13321 tcacttatac aatatttcac ccttatccta tgtatgaaat ctcgaacact catattcaac
    13381 attttaagta tatccaatta tatattagcc aacggaggat ctcaagaaat cttatactgt
    13441 ttatgcacaa gcatatctaa ttatatatat atttgctgaa gtaagactta taaatactca
    13501 tactcgatat atacaagcat acctaattat tgtatatata ttcaccgaag gagggactta
    13561 taaacactca tactcgatat acgcaagctt atctaattat atatacattt gctgaagtaa
    13621 gaatcataaa tactcatact caatatgta
//
LOCUS       DAORYL010000080        13600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_3564_length_13600_cov_13.456478, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000080 DAORYL010000000
VERSION     DAORYL010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13600
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3564_length_13600_cov_13.456478"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1217
                     /locus_tag="PLV81_12375"
     CDS             96..1217
                     /locus_tag="PLV81_12375"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOM10939.1"
                     /translation="MPYTFELGPIRPPSEAYSILIRVTRNCPWNKCAFCHTYKGQQFS
                     RRPIQDVIADIDAIYTLAQRILDVASHGITMHTLQKAKGNDDTPLYYYEQVAFWLQYG
                     MKSVFLQDANSLVLSPNDLCTILNHIKERFPTVERITSYARASTISKKSLDDLKAIRQ
                     AGLTRLHIGMESGSDTVLALVNKGTTQQQLIDAGQKAVEANFDVSYYYMPGLGGKEYM
                     EENALQSALVINNVNPTFVRLRSTVPIPGTPLYEMMEQGTWTPLSEVEKVQEIRLFIE
                     HCNGISSTLTSDHMMNLLEDVEGTFPHDKATMLATIDEFLSMQQEDQEKFIVGRRVGI
                     YRYLKDYYPNFKVDQLYYSIKHQYGSIDKAVMDILKSFI"
     gene            complement(1270..1707)
                     /locus_tag="PLV81_12380"
     CDS             complement(1270..1707)
                     /locus_tag="PLV81_12380"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOM10940.1"
                     /translation="MPEFSYAKGRILESIAFVTCEMMEIEQEYADKKYSEYSSNTKLQ
                     KIIDRTIENILTAIIEMSGAILAENNIKTDSYAEVLRKIGSFFEFSDKECEILASFAI
                     QRNRLAHRYLNFRWQVVTFYNNNKDIITRLVQKMYEYESKKHE"
     gene            complement(1700..2137)
                     /locus_tag="PLV81_12385"
     CDS             complement(1700..2137)
                     /locus_tag="PLV81_12385"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOM10941.1"
                     /translation="MNKEQLIELLKEFFTRHEHMWPVSMAFLYGSWAHDMQRNDSDID
                     IAVMFKENNPNEIFDYINAISLSLSDHLHRDVNVLYIDDDISKPMLHYNAIVKGEVLY
                     FDDFTHYVDVLLKALEQMEDFSMFGLSWQKQIAEKNLKDLTNA"
     gene            complement(2142..3410)
                     /locus_tag="PLV81_12390"
     CDS             complement(2142..3410)
                     /locus_tag="PLV81_12390"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOM10942.1"
                     /translation="MGCTVIIGTQWGDEGKAKMIDYYSQKADIIVRYQGGANAGHTVV
                     VNGKKHVFHLIPSGILHEGTICVIGNGVVLDPVQLIEECQLLEKEGIDVRKRLYISDA
                     AHLLLPFHKEMDAAMEEARSNKIGTTKRGIGPCYADKCLRVGIRVGDIFDEEYLKERL
                     STALAVKNTQFERCYNRKGFSFDEIYDLLMQFKAYAGNLAINTPYYLHSEAKKGKNIL
                     LEGAQGNALDIDHGTYPFVTSSNPSIGGAIAGSGINPFLINEVVGITKAYVTRVGEGP
                     FPTEEKGEAGEIMRQRGGEFGATTGRPRRCGWFDMELLKHTIRINGITTIALTKLDVL
                     SGFETIKVAVGYTINGKKIDYFPSAMQDKIVPVYEEIPGWKEDISQCTSFKELPRNAQ
                     NYVKYIQDSLQVPISIISVGPDRNNTFEMK"
     gene            complement(3502..4104)
                     /locus_tag="PLV81_12395"
     CDS             complement(3502..4104)
                     /locus_tag="PLV81_12395"
                     /inference="COORDINATES: protein motif:HMM:NF013695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOM10943.1"
                     /translation="MMDNLYQKALTLSYFTVGYNILEGVASVIAGAFAGSIALVGFGL
                     DSFVESLSGIIMIWRFRKHGIVSLEEEEEIEHRALKLVGYTFFILAGYILYESVTKLY
                     YVEIPDTSLVGIIIAVLSIIIMPVLYIKKVQVGKTIGSKSLLSDAKETLACTFLSIAL
                     LLGLGLNYLWGLWQADPIVGIIVALYLVKEGREIVTDEED"
     gene            complement(4118..4924)
                     /gene="fdhD"
                     /locus_tag="PLV81_12400"
     CDS             complement(4118..4924)
                     /gene="fdhD"
                     /locus_tag="PLV81_12400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HOM10944.1"
                     /translation="MDIQLETVQNVKAFQYQDSAFEEVTIPVSTEYGITIAINGKPFV
                     TIACSGSELVELATGFLVAEGIIASKSEIEGMWFDEKILTMHVTTKETDAIVQRLFTI
                     RSIVSGCGNASQKFEGLKKLTPPEVSPETIIKIGKQFYQTSELHKLTHGVHSAGLYKT
                     TGECIAFYDEIGRHSAIDKLVGYALYNDIPLHNKIVYSTGRISGEIIQKAIVSGFPII
                     ATKGSPTSLAIQLANEYNVMLIAKVRVNRFSVFTTSIDLVSVFGVGLTLR"
     gene            complement(4908..5276)
                     /locus_tag="PLV81_12405"
     CDS             complement(4908..5276)
                     /locus_tag="PLV81_12405"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOM10945.1"
                     /translation="MKNDILKNHFLHPYNVGIVENYDHKIIVKSDQCNDVITFTVTIT
                     HNTIDDIKFEAYGCGYAIAGASLVSDAVKGKDIDLGIRDVEEMLNTITIPDSNLRCIH
                     LSLKAIKQLKDNLIAHGHTA"
     gene            5652..6003
                     /gene="ssrA"
                     /locus_tag="PLV81_12410"
     tmRNA           5652..6003
                     /gene="ssrA"
                     /locus_tag="PLV81_12410"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(6006..6656)
                     /locus_tag="PLV81_12415"
     CDS             complement(6006..6656)
                     /locus_tag="PLV81_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10946.1"
                     /translation="MMVYSHYNRLYNIFIILITLSIYITCSSGRPKELHEDLPKLKTI
                     KKVAVMVRIPSSSPIEYARYTKTMQAMIAGYKHTKEMIVISEDIPALTVFQSNDDRFF
                     QTSLDGEFLYFKATGIVNSYCFKNKTELQSLFEKYGCDLLVVYEPYGVVSYGMGFIDY
                     DSVMVILNKDLKITYFDYNHDRKETNEFSTEVLWDFLLNEINTRCVNTLIDLGFIK"
     gene            complement(6810..7385)
                     /locus_tag="PLV81_12420"
     CDS             complement(6810..7385)
                     /locus_tag="PLV81_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOM10947.1"
                     /translation="MKNVIFAGVGGQGVILASKILMQVAMNAGYDVKESEVHGMAQRG
                     GSVDCHVRFDKKVYSPLIEKGTADFVVALELLEAMRKLDYLSPQGMLIVNRFKVNPAP
                     VEAGLAKYPDDIESWLCSNVPRCHVVDTTQALKEAGSNRALNIVMLGVLSNFLEFDQK
                     AWEEAIRQSVKEKFVDMNLKAFNLGKSITVS"
     gene            complement(7399..8955)
                     /locus_tag="PLV81_12425"
     CDS             complement(7399..8955)
                     /locus_tag="PLV81_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOM10948.1"
                     /translation="MQKKVMLGNEAIARGAYEAGCIVAAAYPGTPSTEILETIAAEYP
                     DIKAQWSPNEKVALEVVAGASVAGARAMAAMKHVGLNVAADPLFTFSYTGVNGGFVII
                     NADDPGAWSSQNEQDNRHYARAAKLPMIEPTSPEEARKYTKLAFELSEQFDRPVIVRI
                     TTRIAHSQGIVTLEEPKKSELKPFVRNPQKYVMIPAHARPRHVFIEEQMLKLKEYSNT
                     CELNTIEWNSKKRGIITNGVAYQYVKDVCPDASILKIGVAWPLPDAKIHEFCSNVEEL
                     YVVEELDPFIEDYVRALGYNPIGKEIIPICGELTPEIIDMAINKKRPKNEIPVYSGSI
                     PGRPPALCKGCPHGFVFEVLKKLDVVVNGDIGCYTLAVLPPYSAMHTQGCMGASIGMH
                     LGFEKARGDEMARNSVAVIGDSTFVHSGVTPLIDLVYNKGTGTVIILDNRVTAMTGHQ
                     DHPATGRTLKGEETHELDLELLCKAVGVKRVRKIDPKNIEEFEKAVREEIAAKEPSVI
                     ISLRKCILTK"
     gene            complement(8960..9391)
                     /locus_tag="PLV81_12430"
     CDS             complement(8960..9391)
                     /locus_tag="PLV81_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOM10949.1"
                     /translation="MNVTQLSLFLENKPGHLLNALKVLADENVNILTLTIAEVADFGI
                     VRMIVNKPQKALEALRQHKFVCSPTDVLAIELDDKPGSLLKALEIFSKRNLNIEYMYA
                     FTEKRGDKAVMIFKFADIETAKKALLEEGFNVVKKIDLIGE"
     gene            complement(9420..10721)
                     /locus_tag="PLV81_12435"
     CDS             complement(9420..10721)
                     /locus_tag="PLV81_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOM10950.1"
                     /translation="MFNKQYEAMEVKKLKELQLERLKWSLKHAYNNVPYYRQSFDKAK
                     FNPDKFSSLDDLKHVPFLMKQAMRDSYPFGLFAVPMEKVIRIHSSSGTTGNATVVGYT
                     KNDIEVWAEVMARTIAAAGGTEKDIIQVAYGYGLFTGGLGVHYGAEKLGATVIPISGG
                     NTERQLMLIKDFGTTMLACTPSYAINMADFVEKKMKDFDFKKTKLKAGIFGAEPWTES
                     MRKEIESRMGIDAYDIYGLSEVIGPGVSCECSEKSGLHVFEDHFYVEVIDPETGEVLP
                     EGEKGEIVYTSLTKEACPVIRYRSRDITRIYRDACKCGRTLIKMEKVTGRSDDMLIIR
                     GVNVFPMQIEAVLMEMEGTEPHYQIIVDRKGALDDVEIMVEVNEKIFTDELGALKGLA
                     ERIEQRIRSALGISAKITLVEPSTLQRSEGKAKRVIDKRQL"
     gene            11008..11829
                     /locus_tag="PLV81_12440"
     CDS             11008..11829
                     /locus_tag="PLV81_12440"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOM10951.1"
                     /translation="MKFRLLSLVISLFVFTEVLLADDYIEMLKNGTWEQRITAMYTLG
                     YSGNKKAFWYLVKYLDQSFQSENQSLGVRVRQAAAISLGRLKDDRAIPYLVERYKKEE
                     NTEVKRAIIFALRFFKPTPEAITVVKQGLADSNKDIKWEALMVSSVYCDSSLASDIAQ
                     LANSDDPEIKVIAAYIQYTLNQNKPENEALMRKSLTHKSPLVRYWASHFLVKSVGTPA
                     LEDIMKALEIESVWWVANEMENSIYILSRKKFNEEMVEQLGSFRFVVDKKEKQQQ"
     gene            complement(11879..12397)
                     /gene="lepB"
                     /locus_tag="PLV81_12445"
     CDS             complement(11879..12397)
                     /gene="lepB"
                     /locus_tag="PLV81_12445"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOM10952.1"
                     /translation="MIEQKDSFNVFAKVLFAVLGFIVGFIAIRLFLIPVKIDNALMEP
                     TLAVNDYVWFYKTKKVDSGSIVLYKSPLSGKLVGRIIARSGETVEIINKQLYINGVKA
                     APQWAIKKSDTRIFPSRFVNRDNMNTITVPDGHVFILNDNWDTPNDSRLFGVIAIENI
                     VGKYLFKISLKK"
     gene            complement(12408..13589)
                     /locus_tag="PLV81_12450"
     CDS             complement(12408..13589)
                     /locus_tag="PLV81_12450"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOM10953.1"
                     /translation="MSVATYIAEAMTRSSWIRKMFEEGARLKAQFGEENVYDFSLGNP
                     DVTPPKSFFSTLQSLASQPDPKAHGYMPNAGFMSARTTIAKKVAKDHSIDISGDYVIL
                     TCGAAGGLNVALKTILNPGEEVIVPKPYFVEYGFYITNHNGIINLVPTKSDFALDIDA
                     IERAINEKTRAVLINSPNNPTGIIYSHEDITRLAALLQRYSEKFGKPIYLLSDEPYRE
                     LVYDNSTVPSILSHYTHSMVVSSFSKTLSLPGERIGYIAINPSMPEADIVMAGLILCN
                     RILGFVNAPALMQRVVEKIAFDTVDVKAYSTRRDMICEGLQKAGYSFVKPKGAFYVFC
                     KSPLEDDVVFVKHLLNFNILAVPGVGFGGPGFFRLAFCVPEKVIEKSMPQFKKALESL
                     S"
BASE COUNT         4108 a         2964 c         2249 g         4279 t
ORIGIN      
        1 aacattgaac atagaatcct atcaacatcc cctcaatttc tcggaaaatt ttttcttttt
       61 ttgatatact aattaatcca gcaggagaaa ttgccatgcc atacacattt gaattaggtc
      121 ccatcaggcc tccatctgaa gcgtacagca tattgattcg tgttacacgc aattgcccat
      181 ggaacaaatg tgcattctgc catacatata aaggacagca attttcacgg cggcctatcc
      241 aggatgtcat agctgatatc gatgcaatat atacactggc acagcgtatt ctggatgtag
      301 cttctcatgg cattaccatg cacacattgc aaaaagcaaa aggcaatgat gatacgccac
      361 tgtattatta cgagcaggtt gccttctggc tgcagtatgg catgaagtca gtttttttac
      421 aggatgctaa ttcgctggta ctatcgccca acgatttatg tactattctt aatcacatta
      481 aagagcgttt cccaaccgtt gagcgtatca catcgtatgc cagggccagt accatcagca
      541 aaaagtcact ggatgattta aaggcaatac gccaggcagg gctcacgcgt cttcatatag
      601 gcatggaatc tggctctgac accgtgctgg cactagtcaa caaaggaaca acacaacagc
      661 aactcattga tgcagggcag aaggctgttg aagctaactt tgatgtatca tactattaca
      721 tgcctggatt gggcggcaaa gagtatatgg aagaaaacgc ccttcaaagt gcccttgtta
      781 taaataatgt aaatccaacc tttgttagac ttcgcagcac cgtgcctatc ccgggaacac
      841 cactgtatga aatgatggaa caaggaacat ggacaccatt gtccgaagtt gaaaaagtac
      901 aggaaatacg actctttata gaacattgta acggcatcag cagcacactg acaagtgacc
      961 acatgatgaa tttacttgaa gatgtagaag gaacatttcc tcacgataaa gcaaccatgc
     1021 ttgcaactat agatgaattt ctttcaatgc aacaggaaga tcaggaaaaa tttattgttg
     1081 ggcgtagagt gggcatatat cgatatttaa aggattatta tccaaacttc aaagtggatc
     1141 agctgtatta ttctatcaaa catcaatacg gcagcattga caaagcagta atggatattt
     1201 taaagagttt tatatagaat agataggata ctttataaat aactcactac aagatctaaa
     1261 acactttact tattcatgtt ttttagactc atattcatac atcttttgta ctaatcgggt
     1321 tataatatct ttgttgttat tataaaatgt aacaacctgc cacctgaaat taaggtatct
     1381 atgtgccaat ctatttcgct gaattgcaaa tgatgctaat atctcacatt ctttatctga
     1441 aaattcaaaa aacgaaccaa tttttcgcaa tacctctgca taactgtctg tttttatatt
     1501 gttttctgct aaaattgcac cactcatttc aataatagct gttaaaatat tttctattgt
     1561 tctatcaata atcttttgta atttagtatt acttgaatat tcactgtact ttttatcagc
     1621 atattcttgc tcaatttcca tcatttcaca tgtaacaaat gctattgatt ccagtattct
     1681 tccttttgca taactgaact caggcattag ttaaatcctt taagtttttt tctgcaattt
     1741 gcttttgcca ggataatcca aacatgctaa aatcttccat ctgttccaga gctttaagca
     1801 atacatcaac atagtgagtg aaatcatcaa aatacaaaac ttctccctta acaatagcat
     1861 tatagtgcag catgggcttt gaaatgtcat catctatata taaaacgtta acatctcgat
     1921 gtaaatgatc acttagtgat aatgaaattg cattaatata gtcaaaaatt tcattggggt
     1981 tattctcttt aaacataact gcaatatcta tgtcagaatc atttctctgc atatcatgtg
     2041 cccatgaacc gtacaaaaaa gccattgata ccggccacat gtgttcatgc ctggtaaaaa
     2101 attcttttaa taattctatc aactgctctt tgttcataaa actacttcat ttcaaatgta
     2161 ttgttacggt caggcccaac ggaaataatg gagataggca cctgcaaact atcctggata
     2221 tattttacat aattctgcgc atttctgggc agctctttaa atgatgtaca ctgtgatata
     2281 tcttctttcc atccagggat ttcttcataa acaggcacaa ttttatcctg catagctgac
     2341 gggaaataat caatcttttt cccattaatc gtatatccaa cagctacttt gattgtttca
     2401 aaacctgata atacatctaa tttagtcaat gctatggttg ttattccatt tatgcgaatg
     2461 gtatgcttta aaagctccat atcaaaccag ccgcaacgac gtggccgccc ggttgtagcg
     2521 ccaaattcac caccacgctg gcgcatgatc tcaccagctt cacctttttc ttcggttggg
     2581 aatgggcctt cgccaacccg ggtaacatac gccttggtga tgccaactac ttcatttatc
     2641 aagaaggggt ttatacctga acctgctatc gccccaccta tactgggatt tgatgaggta
     2701 acaaacggat aggtgccatg atcaatatca agggcatttc cctgcgcacc ttccagtaaa
     2761 atatttttcc cttttttggc ttcagaatgc aaataataag gagtgttaat agccagattg
     2821 ccagcatacg ccttgaattg catgagaagg tcatatattt catcaaatga aaaacctttc
     2881 ctgttgtagc agcgttcaaa ctgagtattt ttcacagcaa gcgcagttga aagcctctcc
     2941 ttgaggtact cttcatcaaa gatatcacca actctgatac ccacccgcag acatttgtca
     3001 gcataacagg gcccaatacc acgctttgta gtaccaattt tatttgaccg tgcttcctcc
     3061 attgcagcat ccatctcttt atgaaatggc aaaagaagat gagctgcatc agagatatat
     3121 aatcgtttgc ggacatcaat gccttctttt tcaagtagct ggcattcttc aataagttgc
     3181 acgggatcca gaaccacacc attgcctatc acacatattg taccttcatg caaaatacca
     3241 gacggtataa ggtggaacac atgttttttc ccattaacca ccactgtatg cccagcattg
     3301 gcaccaccct ggtaccgtac aataatatcc gccttctgcg aatagtaatc aatcatctta
     3361 gctttgcctt catcacccca ttgagtgcca ataatgactg tacaacccat atcaaattcc
     3421 tttctgattt tattttataa ttgtaacgcc aaccctgcca cgctacatga atatataaaa
     3481 ataacaagct tttttcaact attaatcctc ttcatcggta actatctccc tgccctcttt
     3541 tactaaatac agtgctacaa taattccaac aatagggtca gcctgccaca ggccccacag
     3601 gtaattaagt cccagaccta acagtagcgc aattgaaaga aatgtgcagg ccaacgtttc
     3661 ttttgcatct gaaagcaaac ttttactgcc tattgtcttc cctacctgca cctttttaat
     3721 gtagagaacc ggcataataa taattgatag cacagcaata ataatcccaa ccaggcttgt
     3781 atcgggaatt tctacataat ataattttgt taccgactca tagagtatat accctgctaa
     3841 aatgaaaaaa gtataaccca cgagtttcag cgctctgtgt tcaatttcct cttcctcttc
     3901 cagtgaaaca ataccatgtt ttctaaaacg ccatatcatg attatgcccg atagtgactc
     3961 aacaaagcta tcaagcccaa aaccaaccag agcaatactg ccggcaaaag cacccgcaat
     4021 aacagaggct acaccttcta atatattata cccaactgta aaatatgata atgttaatgc
     4081 tttttggtaa aggttatcca tcatgtttcc ttaataatta tctgagcgtt aatcccacac
     4141 caaatactga tacaagatca atcgacgttg taaacacact aaaacggttt actctcacct
     4201 ttgcaatcaa cattacattg tactcatttg caagttgtat tgccagtgat gtaggtgagc
     4261 ctttggttgc aataatgggg aatcctgata caatggcttt ctgaataatc tcaccggata
     4321 ttcttccagt tgaatacact attttattat gaagtggaat atcattatat aatgcatacc
     4381 ccaccagctt atcaattgca ctgtgccttc ctatttcatc ataaaaggca atacattcgc
     4441 cagtagtttt gtacaaaccc gcactatgaa caccatgggt aagcttgtgt aattctgatg
     4501 tttgataaaa ctgtttccca atttttataa tggtttcagg tgaaacttct ggtggtgtta
     4561 atttttttaa cccttcaaat ttttgcgatg cattcccaca gcctgagact atagaacgaa
     4621 tggtaaaaag acgctgaaca atagcatctg tttcttttgt tgtaacatgc attgttaaaa
     4681 ttttttcatc aaaccacatt ccttctattt cacttttact ggcaatgata ccttcagcaa
     4741 ccagaaaccc cgtagcaagc tccacaagct ctgagcctga acacgcaatg gtaacaaagg
     4801 gcttaccatt gattgctatg gtaataccat actctgtact tactggaata gttacttcct
     4861 caaatgcgct atcctgatac tgaaaagctt ttacattttg taccgtttca agctgtatgt
     4921 ccatgtgcta ttaggttatc ctttaattgc tttatggcct tcaatgaaag atgtatgcac
     4981 cgtagattag aatctggaat tgttattgtg ttgagcattt cttccacatc gcgtatcccc
     5041 agatcaatat ctttgccttt aacagcatca ctcaccagtg atgcacctgc tatcgcatag
     5101 ccacatccat acgcttcaaa ttttatatca tcaattgtat tatgtgtgat agttactgta
     5161 aaagtaatca catcattaca ttgatcactt ttgacaataa ttttatgatc ataattttcc
     5221 actatcccca cattgtatgg atgcaaaaaa tggtttttca gaatatcatt tttcatattt
     5281 caaaattacg cttattcatc atagtacaat agaatattct actacttttt tcctccaata
     5341 tctaatttaa aataaaacaa catataccag tcactatcga cataattatt aaaatcttca
     5401 ttgtggtttt tctatacttg atacctccct catataaaaa atgaaataat ttacctcacc
     5461 cttaagcact tcttatatga ctcactgaat tccttccaaa ccagagcctc caatattaca
     5521 gccattgaaa agttctcacc aaaccacgta aaataataga ataatgattt tgcatcaaaa
     5581 tatcatatac acaaaaaatt atcccaataa atattgacat ttgtgcatct tattgtatat
     5641 tatatatgca aggggacgat cttggtttcg actgcaatgg cgtgtctaca gttgcatgcc
     5701 gagtttctgc tggctcgtaa aaactggcag gttaaaataa gtgcaaacaa cgatttagca
     5761 ctcgcagctt aatccctgcg ggcgtcttta tcgatcccgc tcctctgggg atgataaagg
     5821 cgtgagatac agaggatagt tattgagttt tgatcctggc tcaatagcga aattttaagg
     5881 atttgctcac tgaggcgggt tcctgttccc ctaaccagtg ataagataaa cgaacaggat
     5941 aagcatgtag aggctgtagg tgtgtgttgc aggacgcggg ttcgactccc gccgtctcca
     6001 aaaagtcatt taatgaagcc aagatcaatt aatgtattta cacaccgtgt attaatttca
     6061 ttcagcagaa aatcccacaa tacttcagtg gaaaattcat ttgtttcttt tcggtcatga
     6121 ttgtaatcaa aatatgtaat tttcaaatct ttatttaaaa taaccatgac tgaatcataa
     6181 tctatgaatc ccattccata cgatactact ccatatggct catagactac cagtaaatca
     6241 catccatact tttcaaataa cgattgcaat tcagttttat tcttaaagca atatgagttc
     6301 acaatccctg ttgccttgaa atataaaaac tcaccatcca gagatgtttg aaaaaatctg
     6361 tcatcatttg actgaaaaac agtaagtgct ggtatgtcct ctgaaataac aatcatttct
     6421 ttggtatgtt tataaccagc aatcatcgcc tgcattgttt ttgtatatcg tgcatactca
     6481 atggggctgc ttgagggaat tctaaccatc acagctactt ttttgatggt tttcaacttg
     6541 ggtagatctt catgaagttc tttaggtctt ccagaactac atgtaatata tatagacaaa
     6601 gtaatgagaa taataaaaat gttataaagc cgattgtaat gtgaatatac catcaatggc
     6661 cttacctccc ttacaatagg atttactaac tcacctaaag ccttagcttc aatatcagag
     6721 cagcctctaa ggggctaaag aagcatatta cctcttgatc attatgatat aggaagcccc
     6781 aaaatatctt ttctttaaat ttataaaaat caggacacag taatactttt ccctaaatta
     6841 aaagctttca ggttcatatc tacaaatttt tctttaacac tctgtcgtat tgcttcttcc
     6901 catgccttct ggtcaaattc aagaaaattg gataaaaccc caagcattac aatattgagc
     6961 gccctgtttg agccagcttc ttttaatgct tgcgttgtat caaccacatg gcatcggggg
     7021 acattgctgc atagccagct ttcaatatca tcaggatatt ttgcaagccc tgcttccaca
     7081 ggggcagggt ttactttaaa cctgttaaca ataagcatcc cctgtggtga cagatagtcc
     7141 agttttctca ttgcttcgag caactcaagt gccaccacaa aatcggctgt tcctttttca
     7201 ataagtggtg aatatacctt tttatcaaat ctgacatggc aatcaacact acctccacgc
     7261 tgtgccatgc cgtgaacctc agactctttt acatcatacc ctgcattcat agcaacctgc
     7321 attaagattt tacttgcaag tattaccccc tgtcccccaa ctcctgcaaa aattacattc
     7381 ttcatacttt tctaccctct actttgtcaa aatacattta cgcaacgaaa taatcactga
     7441 tggctcttta gctgctatct cttcacgaac agctttttca aattcttcta tattttttgg
     7501 atcaatcttc cgtactcgtt tcacaccaac tgccttacat aaaagctcta aatccagttc
     7561 atgcgtttct tcgcctttta acgtccttcc tgtagctgga tggtcttgat ggcctgtcat
     7621 tgctgttaca cggttatcca gtattatgac agtacctgtg cctttattat agactaaatc
     7681 aattaaaggt gtaactcctg aatgaacaaa tgttgaatcg ccaatgactg caacactgtt
     7741 tcgcgccatt tcatcaccac gcgctttttc aaaacctaaa tgcataccaa tgcttgcacc
     7801 catacaaccc tgagtgtgca ttgcagaata tggtggcaac actgccaggg tatagcagcc
     7861 aatatcacca ttaacaacaa catctaattt ttttaatacc tcaaatacaa agccatgagg
     7921 gcacccttta cacagcgccg gcggtcttcc tggtattgaa ccagaatata ccggtatttc
     7981 attttttggc ctcttcttat taattgccat atcaattatt tcaggagtta actctccaca
     8041 tataggaatt atttctttac ctattgggtt gtatcctaat gcacggacat aatcttcaat
     8101 aaatgggtca agctcttcta ccacatacag ctcttcaacg ttgctacaaa attcatgaat
     8161 cttagcatca ggaagaggcc atgctacacc aatttttaat atagacgcat ccgggcacac
     8221 atccttaaca tactggtatg caacaccatt ggtaataatt ccgcgctttt tagaattcca
     8281 ttctatagta ttcagttcac aggtatttga gtattctttc aacttcagca tttgttcttc
     8341 aataaaaaca tgtcgtgggc gggcatgtgc aggtatcatg acatattttt gtggatttcg
     8401 tacaaacggt tttaattcgg atttttttgg ttcttctaaa gtaacaatac cctgagaatg
     8461 agcaatacgg gtagtaatgc gtacgataac aggacgatca aactgttcag ataattcaaa
     8521 cgctaattta gtgtatttac gcgcttcttc agggcttgtt ggctcaatca tgggtaattt
     8581 tgcagcacgg gcataatgtc tgttgtcctg ctcattctgt gaactccatg ctccggggtc
     8641 atccgcatta ataatgacga aaccaccatt gaccccggtg tatgaaaatg taaaaagtgg
     8701 gtcagctgct acatttaatc ccacgtgttt catggctgcc attgcacggg caccggcaac
     8761 cgaagcaccc gctacaacct ctaatgccac cttttcgttg ggtgaccact gtgctttaat
     8821 atccggatat tctgcagcta ttgtttccag aatttctgta ctgggcgtac cgggatacgc
     8881 agcagcaact atacacccgg cttcatacgc accacgggca atagcttcat tacccagcat
     8941 tacctttttt tgcattgttt tattctccaa tcagatcaat cttttttact acattaaatc
     9001 cttcttcaag aagagctttc tttgctgttt caatatcagc aaacttaaat atcattacgg
     9061 ctttatcacc tcgtttttca gtaaatgcat acatatactc aatattcaaa ttgcgtttgg
     9121 aaaatatttc cagtgctttc agcaatgaac caggtttgtc atcgagttca attgccagta
     9181 catcagttgg cgaacaaaca aatttatgct gccgaagtgc ttccagtgct ttctgtggtt
     9241 tgttgacaat cattctgact ataccaaaat cagctacttc cgcaatggtc agtgtgagta
     9301 tattaacatt ttcatctgca agtactttca aagcattaag tagatgccct ggtttgtttt
     9361 caagaaaaag cgataattgt gtaacattca ttgttttcct ccattcatta atctttttat
     9421 tagagctgtc gcttatcaat tactcttttt gctttgcctt cactgcgctg caacgtgctt
     9481 ggctcaacca gtgtaatctt tgcacttata cctaaagcac ttcgtatgcg ctgctcaatt
     9541 ctttctgcca gtcccttcaa ggcacccaat tcatcggtaa atatcttttc atttacctca
     9601 accataattt ccacgtcatc cagtgcacct ttgcggtcta caataatctg atagtgtggt
     9661 tcggtacctt ccatttccat gagcactgct tctatctgca tcgggaatac gttaacaccg
     9721 cgtatgataa gcatatcatc gcttcggcct gtaacctttt ccatctttat taaagtacgc
     9781 ccgcacttgc acgcatcacg ataaatcctg gtaatatcac gtgaacgata ccgtataaca
     9841 gggcacgctt ccttggtaag cgatgtatac acaatctcac ctttttcccc ttccggcaac
     9901 acctcacctg tttcaggatc tatcacttca acataaaaat gatcttcaaa cacatgtaat
     9961 ccggactttt cgctgcactc gcacgatacg ccgggtccaa tcacttcgct caggccataa
    10021 atatcgtagg catcaatacc catacgactt tcaatctctt ttcgcatgct ttcagtccat
    10081 ggttcagcac caaaaatacc tgctttcagt ttggtctttt taaagtcaaa atccttcatc
    10141 tttttttcaa caaagtcagc catgttgatt gcatacgaag gtgtacatgc cagcatcgtt
    10201 gtaccaaaat cttttattaa catcagttgt cgctcagtat tgccgcctga aattgggata
    10261 acggtagctc ccagcttttc tgcaccatag tgcacaccca gaccaccagt aaaaagacca
    10321 tagccgtaag ccacctgaat aatgtccttc tcagtaccac cagctgcagc aatggtgcgt
    10381 gccattactt cagcccacac ctcaatgtca ttttttgtgt atccaactac tgtagcattg
    10441 ccagtagtgc cagatgatga atgaatgcgt ataacttttt ccataggaac agcaaaaagg
    10501 ccaaacggat agctatcacg catggcctgc ttcattaaaa atggcacatg tttcagatca
    10561 tcaagagaag aaaatttatc aggattgaat ttagccttat caaatgactg gcgataatag
    10621 gggacattgt tatacgcatg cttcagtgac catttcaatc gctcaagctg caattccttt
    10681 aactttttaa cttccattgc ttcatactgt ttattaaaca ttgccggctc cttttttatg
    10741 gctatatatt ttttaatttt taagaaagta aattgtattt atatttatta aaattgtcaa
    10801 taaccttgta tatgcaacga aaaaaaatta aattttaatt gagtttttta ttatacccct
    10861 agtatgtttg aattaacagt gcacattcac catcgcatat tctatattta agggcgatag
    10921 ttactgatat tgcactccag tgtgcaaaca aaagaaattg tatatatgtg tacggaatac
    10981 attcaatcat aataagggga ctataccatg aaatttcgct tactatcact tgttatttca
    11041 ttatttgttt ttacagaggt acttctggca gatgattata ttgaaatgct gaaaaacggt
    11101 acctgggaac agcgaattac tgcaatgtac acattaggct attccggcaa caaaaaggct
    11161 ttctggtact tagttaaata tcttgatcaa tcattccagt cagaaaacca gtctttagga
    11221 gtacgggtac gccaggcagc agctatttca ttgggacggc ttaaagatga cagggcaata
    11281 ccctatttag ttgaacgcta caaaaaagag gaaaacactg aagttaagcg cgccattatt
    11341 tttgctcttc gtttttttaa gcccactcct gaggctataa ctgtggtaaa gcaaggcctt
    11401 gccgattcca ataaagacat taaatgggaa gcactcatgg tatcaagcgt atattgtgat
    11461 agttccttag catcagacat tgctcagctt gcaaacagcg atgaccccga aattaaagta
    11521 attgctgcat atatccagta cacgcttaac cagaataagc ccgaaaatga agcgcttatg
    11581 cgcaaatcat taacacataa aagcccactg gtacgctact gggcatcaca ttttctggtt
    11641 aaatcagtag gtactcctgc acttgaagat ataatgaaag ctcttgaaat tgaatcagta
    11701 tggtgggttg caaatgaaat ggagaatagt atctatattc tatcccgtaa gaaatttaac
    11761 gaagaaatgg ttgagcagtt aggttctttc agatttgttg tggataaaaa agaaaaacag
    11821 cagcagtaaa aaattctgca ccagactacc atcggcagtc taataatcac tatacatttc
    11881 actttttaag tgaaatcttg aataagtatt tcccaacaat attttcaatt gcaatcacac
    11941 caaatagcct gctatcattg ggagtatccc agttatcgtt taatataaaa acatgcccat
    12001 caggcactgt tatcgtgttc atattgtccc ggttaacgaa tctggatggg aatatccgtg
    12061 tatcactttt ttttattgcc cactgaggtg ctgcttttac accattgata taaagctgtt
    12121 tattgataat ctctactgtt tcaccagatc tggcaattat cctgcctaca agctttcctg
    12181 aaagagggct tttgtaaaga actatcgaac ctgaatcaac tttttttgtt ttataaaacc
    12241 acacataatc attgactgca agagtgggtt ccattaaggc gttgtcaatc tttaccggaa
    12301 taagaaaaag gcgtatagca ataaacccaa ctataaaccc taacactgca aacaaaactt
    12361 tagcaaaaac attaaatgaa tctttttgtt caatcatggc cattttttta agaaagtgat
    12421 tcaagggctt ttttaaattg cggcatggat ttttcaataa ccttttctgg aacgcagaaa
    12481 gctaatctaa aaaacccggg gcctccaaat ccaacaccag gaactgccag tatattaaaa
    12541 ttaagcagat gtttaacaaa caccacatca tcctccagtg gagatttgca gaacacataa
    12601 aatgccccct ttggcttaac aaaggaatat cctgcctttt gcaacccctc acaaatcata
    12661 tctcgccgtg ttgaatatgc ttttacatca acagtgtcaa atgcaatttt ttccacaacc
    12721 cgctgcatga gggcaggcgc attaacaaac cctaatatcc tgttacataa tataagtccc
    12781 gccataacaa tatcagcttc tggcatggaa ggattaattg caatgtatcc tattcgttca
    12841 ccgggaagtg ataatgtttt tgaaaatgat gagacaacca ttgaatgtgt gtaatgggaa
    12901 aggatgcttg gcactgtact attgtcatat accagttcgc ggtatggttc atccgaaagc
    12961 aggtaaatgg gttttccaaa tttttcagag taacgctgca gtaatgcagc taacctggtt
    13021 atatcttcat gggaatagat aatccctgtt gggttatttg gagaattaat aagcactgca
    13081 cgcgtttttt cattgattgc cctttctatt gcatcaatgt ccagagcaaa atcagatttt
    13141 gttggaacta agtttatgat accattatga tttgtaatat aaaatccata ttccacaaaa
    13201 tacggtttag gcactataac ttcctcaccg ggattcaata ttgttttcaa tgcaacattc
    13261 agtcctcctg ctgcaccaca ggttaaaata acataatcgc cgctgatatc tatagaatga
    13321 tcctttgcaa ctttttttgc aatggtggtg cgtgcagaca taaaccctgc atttggcata
    13381 tagccatgtg cttttggatc aggttgtgac gctaacgatt gcagtgttga aaaaaatgat
    13441 ttcggtggtg taacatcagg attacctaag ctaaaatcat agacattttc ctcaccaaat
    13501 tgtgccttaa gtcgggctcc ctcttcaaac atttttctga tccatgatga tcgtgtcatt
    13561 gcttcagcaa tataggtggc aactgacatg gtaacctctc
//
LOCUS       DAORYL010000081        13434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_3612_length_13434_cov_11.870095, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000081 DAORYL010000000
VERSION     DAORYL010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13434
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3612_length_13434_cov_11.870095"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..1471
                     /locus_tag="PLV81_12455"
     CDS             71..1471
                     /locus_tag="PLV81_12455"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOM10954.1"
                     /translation="MTTVYVRKLKAPHQNESFTIFKPLNNELRQEIQTTVKEIFNKAG
                     GKSLLKSSKDVYIKPNGIDGKPYCFTRPEVVDATVRYWFEAGARNVYIMENSTQCNFT
                     RLVFEITGYADICRKTGARPIYLDEDKTVLFEFKGKSSSQYNHDGYDLTKFEMPVTVV
                     DKLINGKNENLYINVPKLKTHSMAIVTLGVKNQWAFPRHNDRRYDHNYNLHYKLVDIL
                     GYVQPDFTLIEGIEGTIYGHYPATKLADTCVIPFKVLIGGDNVVAADIVASRIFGLDV
                     KDVSHLHIAVKKGFAKGVKSIKDIKIDGSIKEYKKRYPTDLYESFPPDVDIIMGKELC
                     CKEGCRNNPLTLLQVLYHDYNGKGGWSLVIGKGHNKELIKSIRKPVLIAGHCAIEETY
                     TMLVQQLGKGNVYVSGQCNDLAASAAAMFKLMKVNPLKLVNLSPLKSVKLLLLAKLHG
                     TKANVPNLLSRFIKTV"
     gene            1482..2396
                     /locus_tag="PLV81_12460"
     CDS             1482..2396
                     /locus_tag="PLV81_12460"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOM10955.1"
                     /translation="MKKRIVWLIIIVIVVISFLFAAGYYFSGLVITPNVWNYEETYRN
                     ELQYKRFTEHYYANLPKEEFFIDSPNGYKLHAMYIPKGSNKTVIFIHGHTYTLMGDIK
                     YLEIFRSLGFNALLFDSRFHGKSGGSNTTFGKYEKYDVKACVDWISKKTGEYSIIGIH
                     GESMGSAICLLYAAIDEKCSFFIVDGAMADLHELLAYRLKKDFGLPAFPLLHSASLIS
                     GLRGAMFFSEISPVTIINNIKAPILFIHGGNDTDIPVEHAQRLYNRYTGIKRIWICPG
                     ATHSKSIVVNSEEYEKQVKEFLHAIGVI"
     gene            2399..2896
                     /locus_tag="PLV81_12465"
     CDS             2399..2896
                     /locus_tag="PLV81_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10956.1"
                     /translation="MLDYLGIFKAFNDRGIHYIVVGGIAVNLYGIPRMTYDIDILLEM
                     SEHNIKQFIGLIKEWGFKPRVPVSVDDFANEDKRKDWIHNKNMKAFTFINPEWAISEI
                     DVIIDSPIDYIHAYNNAVAITVGDITIPVVSIDDLIDMKRKSDRKHDQIDVEYLERVK
                     NEQKG"
     gene            2880..3059
                     /locus_tag="PLV81_12470"
     CDS             2880..3059
                     /locus_tag="PLV81_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10957.1"
                     /translation="MNKKDEGKGYIYHIEEEVLQEYQKKPIYLRLKWLYMGNYLRMGY
                     NKKIKDTHDVFRKGT"
     gene            complement(3060..5237)
                     /gene="feoB"
                     /locus_tag="PLV81_12475"
     CDS             complement(3060..5237)
                     /gene="feoB"
                     /locus_tag="PLV81_12475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOM10958.1"
                     /translation="MTKKSITIGLVGNPNCGKTTLFNALTGARQKVANWAGVTVERRE
                     GFFHHKGYTIKVVDLPGAYSLTSFSMEELITRNFILLDKPDFIVNVVDAGNLERNLYL
                     TTQLIEIEAPVIIALNMIDEAYDKGLTIKTEKLSELFSMPVVPTVGTKNIGIEQLKNT
                     IVDSYEKQSTHRKIVIPYGNDIEQAIHSLQTIIEKNTVAYPSRWLAISLIEGDSDAEK
                     KISHLPNGDEILKTTVSIRNKLIATFNDDIETVLSDMRYGFISGALKEAVHKKPAKRY
                     DITQKIDAIVLNRYIGFPLLFIMLFILFHLTFILGEYPKQLIEAGVGWISNLFTSIIP
                     DGQLQNLIVQGIIGGVGSVIVFLPNILILFLGISFMEDTGYMARSAFLMDKIMHSLGL
                     HGKSFIPLVMGFGCNVPAIMATRSLENKRDRILTVLINPFMSCSARLPVYILFAGTFF
                     PEHAGIIILFIYASGIVLAFISAKLFNNTILKGKSMPFVMELPPYRMPTIKTMLIHMW
                     DRASSYLRKMGGIILAFSIIIWVLSEYPKPYHIEQEYNNRIQQVKQEYKISLSSLQKQ
                     HANQQVIQELNQRYSTILEDLEIQKRQEMVKYTFIGKTGLLIYPLLKPLGFNWQMGVS
                     LTTGFVAKEVVVSTMGVLYHATDDESNQNLSQKLKNPRYGISKASALAFMIFVMIYIP
                     CLATVIAIAREIGLRWAVFSIFYQVFVAWIVSFALYHVARLII"
     gene            complement(5230..5952)
                     /locus_tag="PLV81_12480"
     CDS             complement(5230..5952)
                     /locus_tag="PLV81_12480"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOM10959.1"
                     /translation="MRRRQGNPFNVTDNSSTSIDTPLSPNMEDYMENISMLLKEHDVV
                     RVKDIAKKLNIKMPSVTAALQKLKDKGLVDYEKYGHVQLTAKGKEVADTIYEKHSFLT
                     EFFKDILGMNTQEAEQQACKLEHHLSSDASNRMQRFVEFYKQEQLDSQQWINRLDSVL
                     EEKRLSDLKEGEKAEIVRILGSGPLKKRLLEMGFRKGEIIHVIKYAPLKDPIEISIKD
                     FLLSIRVEEAHSIIVKPLQNHD"
     gene            6653..7237
                     /locus_tag="PLV81_12485"
     CDS             6653..7237
                     /locus_tag="PLV81_12485"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOM10960.1"
                     /translation="MDYISTYLSKISKLVVEQRHALGISQEYLAELCKVHRNTIAVLE
                     LGESDPTIVTLTNIFMAFGIQRFIIDHNNCHLQMITGVPFVDLLESSIINDKIGFFID
                     SLRLKHNISRDKLSLATGIHRNTIARIENAQVTARLSTIIQIYRYFNVSEVYTTTNCD
                     PNDCTFYGVTLRCIDISQRNSTMLINSSPVAIRS"
     gene            7302..8027
                     /locus_tag="PLV81_12490"
     CDS             7302..8027
                     /locus_tag="PLV81_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10961.1"
                     /translation="MRKGTQLVVIMAIVTVISFFAVPIAAEAPSGSFIIAHLGYVPIA
                     ATGYDFSAIDSENGWNPNFVVLEHKSQGAALNIEYNFNTNPLLFSFGFEYQLLWDKFS
                     VFQDIGGGVYETIKLKTYLNQYMMPSVTIKYLTASGFYMGTGIALKYLAVTEKVKPDN
                     GYDFSLEFDKKLDFWSITIVGYIISINQGLYLDVQGRFGYNLTNNQFSSLKTNSPGGD
                     MKAYLMPRTMYDAAILVGVGMNM"
     gene            8350..8703
                     /locus_tag="PLV81_12495"
     CDS             8350..8703
                     /locus_tag="PLV81_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10962.1"
                     /translation="MKRALGVIAVVAIAFIFIAADKTPSADELIDQLGARHSKVFCKY
                     GVPIKMHIDRGPTKEEDYIIFDYLQFGFVFHREMIRQVLFYPEWKGGKIKNINIGMTK
                     DQVIKIMGTPEEEVA"
     gene            8856..10529
                     /locus_tag="PLV81_12500"
     CDS             8856..10529
                     /locus_tag="PLV81_12500"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM10963.1"
                     /translation="MSFEVPVSLVQFLLPPICFGILLGGFILYVYMYWRYKNSLYFAV
                     AFFAFLGMLFVGSETMILSFGGWLHNRSVSVHFHRLEQVAGLFYLFALPYSLNKLLIL
                     NKRWQKINELLAYVGLAYAVIAALCAYLYPDLFISLTKPKSTWMHYEADYGRGQEGIL
                     YALRDALLGIYIVYSIIAVITDMIVQRNYKYLLFPSLGFFCAFAGAAIDTLHVHTHVF
                     YDFFPTVYFSRFSLGITLAIVFFMSSVTRMFITYAQELEIAHKIISISEKKYRFLVEG
                     TNDCIISFDANLNITTANRTAYEQLAIIKGKESSTSLYDLLYLPEGESDLSLQIIKEK
                     INMLFTEGKPINLKTQLKSRGTLEPREYNIRIEHITIDDRNEILMKAASVLDENMLKY
                     VEAEKMRLSIGNYLIAAEEICNRLVLNLPKYVNKQISSAIKLGLREIIINAIEHGNLN
                     ISFEEKSKATNDGNYLEFVLSRQKDPNYKDKKVTIEFLLNSNKVMYKIEDEGNGFNYR
                     EIIQKIQNTVDEDMLAHGRGLRMAFNIFDEVKFNKKGNQVLLVKHFTKN"
     gene            11025..12317
                     /locus_tag="PLV81_12505"
     CDS             11025..12317
                     /locus_tag="PLV81_12505"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOM10964.1"
                     /translation="MKKPKVIVKQCEDYDVKKIESIIQESIKDLNIQLKDPVFIKPNV
                     VTANKQYIFNSYTHPAVVEAMVNVLRNNSINNITIGESGGYGIPSRLFLKEAGYFDLA
                     QKLHVPVIDLNEHPVVRVKLTKGKWHKEIDLSTYIAQANFKIWMPKLKYHIFASITNA
                     LKLNIGILTHKERMLYHDYRIHEKIVDLLEAGYPDLVVSDAIDITYGFESAPYPVRLG
                     ALIISNNPLAADVVAAYIMNYRPQDVLHLRLAAERGYGSCNIKDIAISGDVDIEKLRK
                     KPKGQSRLFQILQELDTPLKFYSGYAPSTDIICDGGCEAAVKGCLGTIEKRRPGSLKN
                     AKKGAIVTGIYKGDVIIPGGSVLLVGDCTKVEGKLVAKRVLRIKGCPIGARNLFIPVP
                     LLFGLPSPMFDVRDAFLFIINSIQKAFNIVVYRWVLKR"
     gene            complement(12417..13064)
                     /locus_tag="PLV81_12510"
     CDS             complement(12417..13064)
                     /locus_tag="PLV81_12510"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOM10965.1"
                     /translation="MKSNVKSKDYQDFLDDLKGYYEKHDQKEENIPVGTRIRKIREIQ
                     GMDIESLASIAGIDAKYLQDIESLKAFPDLGTIIRLSKALKISTGLIIDEQSGLPYVV
                     IRKNERRKITRKISGKKDHPHYEYISLSSGVISRHMESFIVRLTSNVYYTQDLSAHEG
                     EEFLYVLEGAMKIKLADKEETLHTGDSIFYHSTIPHTLKSISPKPAVILAVLYTG"
     gene            13245..>13434
                     /locus_tag="PLV81_12515"
     CDS             13245..>13434
                     /locus_tag="PLV81_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10966.1"
                     /translation="MLCHLWAIVTDDGSDTVYSAHAYTEFAYCSIDMCCYERLFPCDN
                     HYCSDTIYPLRYALTLSFK"
BASE COUNT         4271 a         2226 c         2627 g         4310 t
ORIGIN      
        1 aaaatgtttt gaatgatgta tgtaaaacca gtactatcta ataaaaattt gatttatggg
       61 aacataagcc atgacaacag tgtatgttcg taaacttaaa gcaccacatc agaatgaaag
      121 ctttactatt tttaagccgc tgaataatga actgcgtcag gaaatacaga caacagtaaa
      181 agaaattttt aacaaagctg gtggaaaatc acttcttaaa agcagtaagg atgtgtatat
      241 taagccaaac ggaattgatg gcaaaccata ttgttttaca cggcctgagg ttgtagatgc
      301 aactgttcgc tactggtttg aagctggtgc tcgtaatgtc tacataatgg aaaactcaac
      361 gcagtgtaat tttactcgtc ttgtttttga aataacaggc tatgcagata tatgccgtaa
      421 aacaggtgcc aggcctatct atctggatga ggataaaaca gttctttttg aatttaaggg
      481 gaaatcttca tcgcagtata atcatgatgg ctatgacctg actaaatttg aaatgccagt
      541 aacagttgta gataagctta ttaacggtaa aaacgaaaat ctatatataa atgtgccaaa
      601 acttaagact cactcaatgg ctattgtaac attgggtgta aagaatcagt gggctttccc
      661 acggcataat gacagaaggt acgatcataa ctataatctt cattataagc ttgtggatat
      721 tcttggatat gtgcagcctg attttacgct cattgaaggg attgaaggta caatctacgg
      781 gcactatccc gcaacaaaat tggctgatac ctgtgtcata ccatttaaag tgttaatagg
      841 tggtgataat gtagtggctg cagatattgt tgcttcccgt atttttgggc tggatgttaa
      901 agatgtgtcg catcttcaca tagcagtgaa aaagggtttt gcaaaaggtg ttaaatctat
      961 taaagatatc aagatagatg gcagtattaa ggaatataag aaacgctatc ccactgactt
     1021 atatgaaagc ttccctcctg acgttgacat tattatggga aaagagttgt gctgtaagga
     1081 aggatgccgc aataatcctt taacactttt acaggtatta taccatgatt acaatggcaa
     1141 gggtggctgg agtcttgtta ttggcaaagg ccataataaa gaattgatta aaagtattcg
     1201 taagccagta ctgattgctg ggcattgtgc aattgaagaa acttatacca tgctggtaca
     1261 acaattgggc aaaggtaatg tatatgtcag tggacaatgt aatgacctgg ctgcttcggc
     1321 tgctgctatg tttaagttga tgaaggtaaa tcctttaaag ctggtgaatc tgtctccttt
     1381 aaaatcggta aagttgttgc tcttagcaaa attacatggt acaaaagcaa atgtaccaaa
     1441 tttactatcg cgttttatta aaacagttta ggagatagta catgaaaaaa agaattgtat
     1501 ggctcattat aatagtcatc gtcgtaattt catttctttt tgctgctggg tattattttt
     1561 caggtttggt tattacccct aacgtgtgga attatgaaga aacgtaccgt aatgaattgc
     1621 aatataaaag atttactgaa cactattatg ctaatttgcc aaaagaggaa ttctttattg
     1681 attcgcctaa tggctataaa ctgcatgcaa tgtatatacc aaaaggtagc aataaaacag
     1741 ttatatttat tcatggacat acgtatacct taatgggcga tatcaagtat ctggaaatat
     1801 tccgttcatt ggggttcaat gcattgttgt ttgatagtag atttcatggc aaaagtggtg
     1861 gttccaatac aacatttggt aaatatgaaa aatatgatgt taaagcctgt gttgactgga
     1921 ttagcaagaa aactggtgaa tatagcatta ttggcataca tggcgagtca atgggatcgg
     1981 caatttgttt attatatgct gccattgatg aaaaatgcag ctttttcatt gttgacggag
     2041 caatggctga tttacatgaa cttctggcat atcgtcttaa aaaagatttt ggtttgccgg
     2101 catttccatt attgcattca gcaagcctga taagcgggtt acggggtgca atgttttttt
     2161 ctgagatatc acctgtcaca ataattaaca atattaaagc accaatattg tttatccatg
     2221 ggggtaatga tactgatatc cctgttgaac atgcacaacg tttgtataac aggtatacag
     2281 ggataaaacg aatatggatt tgcccgggcg caacacattc aaaatcaatt gtagtaaaca
     2341 gcgaagaata tgaaaagcag gtaaaagaat ttttgcatgc cattggggtg atttgataat
     2401 gctggattat cttgggatat ttaaagcatt taatgataga ggtatacact atattgtggt
     2461 tggaggaatt gctgtaaatc tatatggcat accgcgtatg acatatgata ttgatatact
     2521 tttggaaatg agtgaacata atattaagca atttattggt cttattaaag aatggggttt
     2581 taaacccaga gtaccagttt ccgttgatga ttttgcaaat gaagataaac gaaaagattg
     2641 gatacataat aaaaacatga aagcatttac ttttataaat cctgaatggg caatttctga
     2701 aattgatgtt atcatagatt ctccaattga ttatatacat gcttataaca atgcagttgc
     2761 tattacggtt ggtgatatta ctatacctgt tgtgtcaatt gacgatctta ttgacatgaa
     2821 acgaaaaagc gatagaaaac atgatcagat tgatgttgaa tatctggaga gggtaaaaaa
     2881 tgaacaaaaa ggatgaggga aaagggtata tatatcatat agaagaagaa gttttacaag
     2941 aatatcagaa gaagccaata tatttgcgcc ttaagtggct atatatgggt aattatttgc
     3001 gaatgggata taataaaaaa ataaaagata cacatgatgt cttcagaaag ggcacataat
     3061 tatataatca atcgtgccac atgatacaat gcaaagctca caatccatgc cacaaaaacc
     3121 tgatagaaaa ttgaaaaaac tgcccaccgt aggccaattt cacgggcaat ggcaataacg
     3181 gttgcaaggc agggaatgta tatcataaca aatatcatga aggcaagagc acttgctttt
     3241 gatatgccgt accggggatt ttttagtttt tgtgaaaggt tctgattgct ttcatcatca
     3301 gttgcatggt ataaaacgcc catggtgcta actactactt cttttgccac aaaacctgta
     3361 gtaagcgaaa cacccatttg ccagttaaat cctaaaggtt tcagcagtgg ataaataagt
     3421 aggcctgttt tcccaatgaa cgtatattta accatttcct gtcgcttctg aatttctaaa
     3481 tcttccagaa tggtagaata cctttgattc aattcctgaa taacctgttg atttgcatgc
     3541 tgtttttgta atgatgatag tgatatttta tattcctgct taacctgttg aatgcggttg
     3601 ttatattcct gttctatatg atagggtttt ggatattcgc ttaaaaccca tataataatt
     3661 gaaaatgcta aaataatacc acccatcttt cttagataac tggaggctct atcccacatg
     3721 tgaatgagca tggtttttat agtgggcata cgatatggag gaagctccat aacaaatggc
     3781 attgatttgc ctttaaggat ggtattgtta aatagttttg ccgaaataaa tgccagcaca
     3841 atacctgatg cgtatataaa aagaattata ataccagcat gttcagggaa aaaagtgccg
     3901 gcaaaaagta tgtagaccgg aagtctggct gagcagctca taaaaggatt aataagtact
     3961 gtcagtatgc ggtctctttt attttccagc gaacgagttg ccataattgc gggcacattg
     4021 catccaaagc ccataacaag tggaataaaa gatttgccat gcaagcctaa actgtgcatt
     4081 attttgtcca tcaggaatgc agaacgtgcc atgtatcccg tgtcttccat gaagctgata
     4141 cccagaaata aaattaatat atttggcaaa aatacgataa cactaccaac gccaccaata
     4201 attccctgga caattagatt ttgcagttgc ccgtctggta taatactggt aaaaagattg
     4261 cttatccacc ccacacctgc ttcaatcagc tgttttgggt attcccccaa aatgaatgtt
     4321 aaatggaata gaataaaaag catgataaaa aggaggggaa agccaatgta tctattcagt
     4381 acaatggcat ctatcttttg tgttatatca taccgttttg caggcttttt gtggaccgct
     4441 tctttcaagg ctccgcttat gaatccataa cgcatatctg atagcactgt ttcaatatca
     4501 tcattaaagg tagcaatcag cttgttgcga atagatacag ttgttttcag tatctcatcg
     4561 ccattgggca aatgactgat tttcttttca gcgtcactgt caccttctat caggcttata
     4621 gctaaccatc gagatgggta cgcaacagta tttttttcga taattgtctg taacgaatga
     4681 attgcctgct ctatatcatt accatagggg attacaattt tacggtgtgt tgattgtttt
     4741 tcataactat cgacaatagt atttttaagt tgttcaatac caatattttt tgtgccaacc
     4801 gttggaacaa caggcattga aaacagttca ctcagctttt cggtttttat ggtaagccct
     4861 ttatcatacg cttcatcaat catgttaaga gcaataataa ctggagcttc aatctcaatt
     4921 aattgtgtgg taagatatag attgcgttct aaattgccag catcaacaac attgacaatg
     4981 aaatctggtt tgtccaataa aataaaattt cgcgttatca gctcttccat gctgaatgaa
     5041 gtaaggctgt atgcaccagg cagatccaca accttgatgg tatatccctt gtgatggaaa
     5101 aaaccttccc ggcgttccac ggtaacgcca gcccagtttg caaccttctg ccgcgcaccg
     5161 gtcagggcat taaacagggt agttttgcca caattgggat tgccaacaag gccaatggtt
     5221 atgctttttt tagtcatggt tttgcaatgg ttttacaata atagaatgtg cttcttcgac
     5281 acgtattgaa agtaaaaaat ccttaattga aatttcaatg ggatctttta aaggagcata
     5341 ttttataacg tgaataattt cgccttttct gaatcccatt tcaagcaagc gcttttttaa
     5401 agggccactt ccaagaatcc ttacaatttc agctttttca ccttctttaa gatcactcaa
     5461 gcgcttttct tcaagtacac tgtcaagcct gttaatccat tgctggctat caagctgttc
     5521 ctgtttgtaa aattcaacaa agcgctgcat gcggttactg gcgtcactgc tcagatgatg
     5581 ctctaattta caggcctgtt gttctgcttc ctgtgtattc atgccaagaa tgtctttaaa
     5641 aaactcagta agaaatgaat gcttttcata aatagtgtcg gccacttctt tgcctttagc
     5701 agtaagctgc acatgcccat atttttcata gtcaacaagc cctttgtctt taagcttttg
     5761 taatgctgca gtgacgctgg gcatctttat attgagcttt ttagcaatat ctttaacacg
     5821 gacaacatca tgctctttta aaagcattga gatgttttcc atataatctt ccatattggg
     5881 cgaaagtgga gtatcaattg aggtactgga gttatcggta acattaaaag gatttccctg
     5941 tcttcgtctc attctcatgg cgatattaca tttttctatt atagtataat acaatttact
     6001 gaatataata taattagtca agactaatat tatgcattat ataaaatata atttgagcga
     6061 tagttactga gtaagagtga catgtcattt tgaataaagc gaagaatcct aaaaaacgtc
     6121 gatattcttc gtcctgattt tatcgggata aagaagcatt atctttttaa gtaatgagat
     6181 tgcttcgtcc cgattgcatc gggactcgca atgactttgc ggggaaggtc atcttgagtc
     6241 ccgatccaat tgggacgacc gaagaatgac ccagtagcat aaatgccacc caaagctgaa
     6301 agaagcggtc ggtcacttcg atacacacgc tttgcgtgca ctcagtgacc gggaattcga
     6361 taaatgtgct ttgcatgcac tcggtgaccg ggaatccgat acacgtggtt tgcgtgtact
     6421 cagtgaccga aaatccgata cacgtggttt gcgtgtactc agtgaccggg aattcgataa
     6481 acgtgcttag aacgcactcg gtgatcgtat cgtactataa atatatataa taatttaaga
     6541 gaaataaaaa aataccatgt actctgacat ccttgattaa aatgcatggt tatttttttt
     6601 aattacttga aaaatttaat tgtatggttt tacaattgta aaaaggtgaa aattggatta
     6661 tatttctaca tatcttagta aaatttcaaa gcttgttgtt gaacaacgtc atgcactggg
     6721 catatcccag gaatatcttg cagagctttg taaggtacat cgaaacacaa ttgctgtatt
     6781 agagttaggg gaaagtgatc caacaatagt tacattgaca aatattttta tggcttttgg
     6841 aattcagcgc tttattatcg atcacaataa ctgtcactta caaatgatta cgggtgtccc
     6901 ttttgttgat ttattggaaa gctccatcat taatgacaag atagggtttt ttattgattc
     6961 acttcgatta aaacacaata tatcccgtga taagctttca ttggctacgg gtattcaccg
     7021 taatactatt gcgcgaattg aaaatgcaca ggttacagca cgtctgagta ccattataca
     7081 gatatataga tattttaacg tttccgaagt ctatactaca accaattgcg accccaatga
     7141 ttgtacattt tatggtgtca ccttacgctg tattgacata agccaaagaa acagtaccat
     7201 gctgattaat agcagtcctg tagcaatacg cagttgattt taaattctgt acatgcagct
     7261 acattacctg ctataatcaa aaaatttatg gaggttttat tatgcgtaag ggtactcaat
     7321 tagttgttat tatggcaata gtaactgtaa tttcattctt tgcagttccc attgcagctg
     7381 aagcaccatc aggcagtttt attatagctc acctgggtta tgtcccaatt gcagcaacag
     7441 ggtacgattt ttcagcaatt gatagtgaaa acggatggaa tccgaatttc gttgtactgg
     7501 agcacaaatc tcagggtgct gcattgaaca tagaatataa tttcaataca aatccccttt
     7561 tattttcttt tggttttgaa taccagcttt tatgggataa gttctctgtt tttcaggata
     7621 taggaggagg agtgtatgaa acaattaaat taaaaacata cttgaaccag tatatgatgc
     7681 caagtgttac cattaaatac ctgacagcat ctggttttta catgggtacc ggtattgcac
     7741 tgaaatatct tgcagtaaca gaaaaagtta agccggataa tggctatgat ttctctctgg
     7801 agtttgataa gaagcttgat ttctggagta tcacaatagt ggggtatatc ataagtatta
     7861 atcagggatt atacctggat gtgcagggac gttttgggta taacttaaca aataatcagt
     7921 tttctagttt aaaaaccaat agccctggtg gagatatgaa agcatatttg atgccaagaa
     7981 ccatgtacga tgctgccata ctggttggtg ttgggatgaa tatgtaaagt atgtaacata
     8041 ggtatatgtt catgagaggt tgtttaaaaa attttattgc ttgaatatca taaatcgtta
     8101 ttgtctattt ttcattttca ggtgattatg ctataacttt tgttaaagtt attcaattcc
     8161 tgttttagga tttatagcat aacagccatt ttggacaacc tcttatacat atcatattgt
     8221 ataaataaaa atattacttc agtaactatc gcccaaaaaa aataaaatta ttacttgcta
     8281 ttaataatca tttatggcac aataccgaaa tatgttgttt attataataa aattttaagg
     8341 aggcttctta tgaagcgtgc attaggcgta attgccgttg tggcgatagc tttcattttt
     8401 attgctgcag acaaaacacc atcagcagat gaattaattg atcagcttgg tgcgcgtcac
     8461 tcaaaggttt tttgtaagta tggcgttcca atcaaaatgc atattgatag gggtccaact
     8521 aaagaagaag attacatcat ctttgactac ttgcagtttg gttttgtgtt tcacagagaa
     8581 atgatacggc aggtcctgtt ttatcctgaa tggaaaggtg gaaagattaa gaatataaat
     8641 ataggtatga ctaaagacca ggttattaaa attatgggca cgcccgaaga agaagtggca
     8701 tagaatgatg gccggacccg tataacgtat ttagagccgg ataagtattt tgatattatc
     8761 ctctcaacaa gtaacgttgt tacgatggtg agatttgaag tgaaataaca tattaaagca
     8821 tacaataaaa tataaaatga aatcaaagga agagtatgag ttttgaagtg cctgtaagtc
     8881 tggttcagtt tttattgcca ccaatttgct ttggaatact tcttggtgga tttattttat
     8941 atgtgtatat gtactggaga tataaaaatt cgctgtattt tgctgttgcg ttctttgctt
     9001 ttttgggaat gctatttgtt ggcagtgaaa caatgatatt gtcgtttggc ggatggctgc
     9061 ataatcgatc tgtatctgtt cacttccacc gtttagaaca ggttgcaggg cttttctatc
     9121 tctttgctct tccttattca ttaaataaat tgcttattct gaataagcgg tggcaaaaaa
     9181 taaatgagct actggcatat gtgggtctag cgtatgctgt gattgcggca ttatgtgcat
     9241 atctgtatcc tgatcttttt atttcactaa ccaaaccaaa aagtacctgg atgcattatg
     9301 aagcagatta tggaagaggg caggaaggca tcctgtatgc acttcgtgat gcattgctgg
     9361 gaatatatat tgtttacagt ataattgcag ttatcactga tatgattgtc cagcgtaatt
     9421 ataaatacct tctttttcct tcgttagggt tcttctgtgc atttgctggt gctgcaattg
     9481 atacattgca tgttcataca catgtatttt atgatttttt ccccactgtg tacttttcgc
     9541 gtttcagcct tgggattacg ctggcaatag tatttttcat gtcatcagta acccgcatgt
     9601 tcatcacgta tgcacaggaa ctggagatag ctcataagat tataagtatt tccgaaaaga
     9661 aataccgttt ccttgttgag ggcacaaatg attgcattat atcatttgat gcaaatttaa
     9721 acattacaac agccaaccga accgcctatg aacaactggc tattataaaa ggcaaagaat
     9781 cttcaacatc gttgtatgat cttttatatt tgcctgaagg tgaaagcgat cttagcttgc
     9841 agattataaa agaaaaaatt aacatgttgt ttactgaagg aaaacctatt aatctaaaaa
     9901 cacagttaaa atcacgagga accctggagc cacgcgaata taacatacgt attgaacaca
     9961 ttactattga cgaccgcaat gaaatattga tgaaggcagc gtcggtactg gatgaaaata
    10021 tgctgaagta cgtagaagct gaaaagatgc gcctttctat aggaaactat cttatagctg
    10081 cagaagaaat ttgcaacagg ctggtgctta atttgccaaa atatgtcaac aaacagatta
    10141 gcagtgcaat aaaattggga ttgcgtgaaa ttattatcaa tgcaattgaa catgggaatt
    10201 taaatattag ctttgaagaa aaatcaaaag ctactaatga tggtaattac cttgaatttg
    10261 tattgagcag gcaaaaagat cccaattata aggacaaaaa ggtaaccata gaatttttgt
    10321 taaacagtaa taaggtaatg tataaaatag aagatgaagg aaacggattc aattacaggg
    10381 aaataataca aaaaatacaa aatacggtgg atgaggatat gctggcacat ggcaggggtt
    10441 tgcggatggc attcaatata tttgatgagg tcaaatttaa taaaaaaggc aatcaggttt
    10501 tgctggttaa acattttact aaaaattaga gttttgtgat acaattatca tatcttgata
    10561 atttttaatt aaaataaaaa agcatacata ttataatctg aagtagaact acatataaaa
    10621 acacattcag taataattcc ttaaaaaatt atttttttgt ttactataat aaatttcata
    10681 tcattcgtgc ggaataaggc ttttgtaatc aagcatttct tgccttgaaa aagctacgcg
    10741 ataaatactg aaatatattt acttacagat attaaaaata aatcatcact atcctgatta
    10801 ttgcatactt tgataaaatc aacaacaacc tgtacaatag aattgaattt attttttaaa
    10861 ataaggaagt ctcttccaat aaattttatg gtagtgatag aactgtatgg aatactaatg
    10921 agctgtaaaa agtatttata agacagttta ttttaattga caatagttat tatattaaca
    10981 ataatactat aaaatgttgc aatacaattt ttgaggtaaa tgctatgaaa aaaccaaaag
    11041 taatagtaaa acaatgtgaa gattatgatg tcaaaaagat tgaaagcatt atccaggagt
    11101 caattaaaga ccttaacata cagctaaagg atccagtctt tatcaaacca aatgttgtta
    11161 cagcaaacaa acagtatatc tttaactcat atactcatcc agccgtcgtt gaggcaatgg
    11221 ttaatgtttt acgaaataac agtatcaata atattaccat aggtgaatcg ggcgggtatg
    11281 gcattccatc acgcttattt ttaaaggaag cgggctattt tgatttagca cagaagttac
    11341 atgttcctgt gattgatttg aatgagcatc cagtcgttag agtgaagctt actaagggaa
    11401 aatggcataa ggaaatagat ctttctacct acattgcaca ggcaaacttc aagatctgga
    11461 tgccaaagtt aaagtatcat atctttgcat caatcaccaa tgccttgaag ctaaatattg
    11521 gcatactcac gcacaaagaa aggatgcttt accatgacta tcgcatccat gaaaagatag
    11581 ttgaccttct ggaagctggc tatcctgacc tggttgtttc agatgccatt gacattacct
    11641 atgggtttga atcggctccg tatcctgtaa ggctgggagc acttattatt tccaacaatc
    11701 cactggctgc tgatgtggtt gcagcgtata tcatgaacta tcgccctcag gatgtattgc
    11761 atttgaggct tgcagctgag cgtggctatg gttcatgcaa tatcaaggat attgcaataa
    11821 gtggtgatgt tgatattgaa aagctcagga aaaaacccaa gggtcagagc aggttgtttc
    11881 agatattaca ggagcttgat acgccactca agttttattc agggtatgcc cccagtacgg
    11941 atataatctg cgatggcgga tgtgaagctg ctgtcaaagg atgtctgggc accattgaaa
    12001 aacgaaggcc tggttcactt aaaaacgcaa agaaaggagc tattgttacg ggaatataca
    12061 aaggtgatgt gattatcccc ggtggtagtg tattgctggt gggtgattgt actaaagttg
    12121 aagggaagct tgttgccaaa agagttttgc gaatcaaagg gtgccccatt ggcgcacgca
    12181 atctctttat tcctgtacca cttttgtttg gactgccaag cccaatgttt gatgtgcgcg
    12241 atgcttttct ttttattatc aattccattc agaaagcgtt taacattgtt gtatacaggt
    12301 gggtactgaa acgataaaat gatgtgcgat agttactgat aattggttca aatatagtta
    12361 tggtgttgct taaaaaacaa tctagtgcat acggattttg ctgcgaataa aaagtattat
    12421 ccagtataga gcactgctaa aatcacagca ggcttggggc taatgctttt taaggtatgc
    12481 gggattgttg agtgatagaa gatgctgtca ccagtatgca gtgtttcctc tttatcagct
    12541 aatttaatct tcattgctcc ttcaaggaca taaaggaatt cctcgccttc atgtgccgat
    12601 agatcctggg tataatatac attgctggtt aatctgacga taaatgattc catatgccga
    12661 gaaatcacac cagaagataa tgagatgtat tcataatgag ggtggtcttt tttacctgag
    12721 atttttcggg ttatttttcg tctttcattt ttacgtataa ccacatatgg taggcctgat
    12781 tgttcatcaa tgataagtcc tgttgatatc ttgagcgctt tggataaacg aataattgtt
    12841 cccagatcgg gaaatgcttt gagcgattca atgtcctgca aatatttagc atcaatacca
    12901 gctattgagg ctaatgattc aatatccatt ccctgtattt ctctgatctt tcgtatacgg
    12961 gtccctaccg gtatattttc ttctttttgg tcatgttttt cataataccc ttttaaatca
    13021 tccagaaaat cctggtaatc cttgcttttt acattggact tcattggacc ctcacattaa
    13081 tattaaagtg tttatgttgt gtataggggg taataattgt gctgtcaaga atttaattgc
    13141 tctgacacca aacctgcata cgagacttca tatttggcgt aggagtatct tactctgaca
    13201 ccatacttgc ataaggactt aattcattta acacaataat tattatgctc tgtcaccttt
    13261 gggcgatagt tactgatgat ggctctgaca ccgtatacag tgcacatgct tacacggagt
    13321 ttgcatattg cagtattgat atgtgctgct atgaacgtct ctttccgtgt gacaaccatt
    13381 attgctctga caccatatat cctttaagat atgctctgac actgagtttt aaat
//
LOCUS       DAORYL010000082        13263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_3661_length_13263_cov_10.418459, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000082 DAORYL010000000
VERSION     DAORYL010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13263
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3661_length_13263_cov_10.418459"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..277)
                     /locus_tag="PLV81_12520"
     tRNA            complement(204..277)
                     /locus_tag="PLV81_12520"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(241..243),aa:Arg,seq:tct)
     gene            complement(286..360)
                     /locus_tag="PLV81_12525"
     tRNA            complement(286..360)
                     /locus_tag="PLV81_12525"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(323..325),aa:Pro,seq:tgg)
     gene            complement(448..765)
                     /locus_tag="PLV81_12530"
     CDS             complement(448..765)
                     /locus_tag="PLV81_12530"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOM10967.1"
                     /translation="MIKNTFGIEDTIINSLREIFLQYPEIEKVLIYGSRAKGSYSKGS
                     DIDIVIIAPQMDFSHYLKLYAQLDELEIPYTLDVTKYELIGENIKSHIQRVGVEIYKH
                     NHK"
     gene            complement(758..1153)
                     /locus_tag="PLV81_12535"
     CDS             complement(758..1153)
                     /locus_tag="PLV81_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HOM10968.1"
                     /translation="MQRLENFEKAINQLKAAVVQYNEKGLNELEKQGMIQAFEYTFEL
                     AWNLIRDYFLYQGIHDIQGSRDAVKMAFKFGIIENGGIWMEIIKTRNLTAHTYNQMIA
                     DSIVSQIVKSYYAELEKLFKRFMEIKMND"
     gene            complement(1524..2324)
                     /locus_tag="PLV81_12540"
     CDS             complement(1524..2324)
                     /locus_tag="PLV81_12540"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HOM10969.1"
                     /translation="MKKPFFEKPARIFAHRGYSKEYPENTALAFEKAIALGADVIETD
                     AHLTRDGHVVIVHDDELSHITDGKGNVGQLTLQEIKQLDAAYHFSLDGETYPYRGKGI
                     TIMTLEEMLDAFPEQRFNVDLKDNTIVLAEKFCDIVLSHKSEHRVLGASQHGKNLQYI
                     RKRIPTMATSFGLWGVLGFYFLFRSGLLGLFKHFPGDALQIPEYIGPSHLANGALIRM
                     AHERGIQVDVWTVNQERDMRRLLDVNVDGIVTDDVPLLQKVLQDYPLK"
     gene            complement(2321..2869)
                     /locus_tag="PLV81_12545"
     CDS             complement(2321..2869)
                     /locus_tag="PLV81_12545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOM10970.1"
                     /translation="MALQKYPSLFLDRDGTLIKDVGYISNPGRVVFYTCTFDALKEAQ
                     QFFRLFIITNQPGIAKGIVTLDEAITVNTYIQSVFADKGITIESIYMCPHAKEDNCHC
                     RKPKTYFVDQAVAHYNLDVQHSFVIGDHPSDILLAKNAGMQGIYVLTGHGKKHKKEID
                     NCVIVKKNLQYAVDYILKRVQL"
     gene            complement(2870..4759)
                     /locus_tag="PLV81_12550"
     CDS             complement(2870..4759)
                     /locus_tag="PLV81_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4914 family protein"
                     /protein_id="HOM10971.1"
                     /translation="MKDLLSKLIVPEQVKALLQVTNVTVAHTVDDLFELSLKFGNDGV
                     QKVVYDVPGKGEYCEAVVYKVKNGIAVNYTEAYMRRRDPDSMVIGDDLPTDKITYTQR
                     FGKDFNELKKETFDWLQKQDLAVFFFKAGQLDGCYGMAIAPANAGFFCLGLGILQGII
                     DSRQIEGTIHVKCILYIAPVFRHTHFNGKQIVVHYRSENLHEIYSYNLYPGPSAKKGV
                     YSSLLDFGEKEGWVTNHCAVVQVITPYGNKTTIMHEGASGSGKSETNEHIHRDVDGTI
                     KLGVNIVTGEVLSLVLPRGCFLRPVVDDMGLCHPSFQKDDGYLYLKDAEAGWFIRVNH
                     IEQYGTDPDIESLSIHPKVPLMFLNIDAQPFSTALLWEHIEDEPGKSCPNPRFLVPRN
                     ALPDIVNKPVQVHIRSFGVRTPPSTKEKPDYGIIGLFHILPPAIAWLWRLVSPRGHDN
                     PSIVKTGGLESEGVGSFWPFATGKKVNFANLLLEQIIASPKVKYVLAPNQYIGAWQVG
                     FNPQWIMREYLARRGGVKFFADELSKSRCSLLGFSLNKLVIEGVEIEKFLLRPELQSE
                     VGEDAYDRGAQILVDFFKKELMDYYNDPNLLPLGKQIIECFLSDGTIEEYMQLIKDEG
                     LIMED"
     gene            5134..8283
                     /locus_tag="PLV81_12555"
     CDS             5134..8283
                     /locus_tag="PLV81_12555"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOM10972.1"
                     /translation="MILLQDTTNKIDMTDYLYYLEDTTNRLQPKDIVQQQIQSRFKKV
                     HKKINYGFTKSTFWIRFNVKDISSSPTTWYLLLDYPLIENVTLYRKKSGRLQKLAEIS
                     QQQQLNTTDILYTGRIFELNPGKNQNDEYYIKLKTRTTMRFPLSICNINYINKTSSFR
                     MHINGIYLGIIFIMFFFNLFYYFIIRSYNSIFYSMYIIGFGLFVSIQNGIFFEIIPRF
                     PIKYLLPLYMGTGSLVFIFSTLFTMFFLKTRTQLPKINKILQGIIGISLVFLALLPFL
                     DMSCMIQLFAIFGILWSFANFIAGMYSLYTGYRSARFFITGWIFFFIGVLIFALRGFG
                     LLPSNAITLYSVELGSILVIFFLSIAMADQYYIIQQEHSRQQSIIIEKEKALRETQEL
                     LIQNLHQMSTFKDDLINATSSQLRTPLSSIIGITESLLGGIAGELNQKIINNLQLILA
                     SSKRLSRLIHNFLDYSQLKYNTTQLSLTAVDLKSMIDVVIDIIQKLPLSNSITIKNNI
                     PVNLPLVLADKDRLPQALFNILYNSIKLTSNGTITLHSAIQTNDPSKITIAIHEDGKG
                     VLPYIFDTIIHQDIYLNQKTPKNSFLTGIELTIAKQLIELHGTTIAIDTNPDTGTTLS
                     FSLQCADFSPAQDVHAYHKETPVVDNHFIDLASMPYIENIDKTSIPSTQQIARILVAD
                     SDPVLLQSIDNNLVLNGYQVLKAYNGQEVLGILDSGMLPDAMLLDIMLPRTNTLEVIR
                     KIRTTYTIAELPIIVISPIEEPESLMASFEAGANDYIVKPFYMYDIISRLKTQLLLKQ
                     AVASIKRVTQIEKELDMARQIQQTLIPQETPVSGKYEIAHVYIPMDKVGGDYYNFNIK
                     DDNHIGIFISDVSGHGMPAALIASMLQSLIHTGHQAVDDPVSLLHKLNTSLKKNLDDN
                     FLTALYLYIDFESMTCKVSRAGHEPCILFRRSQDAIYEILPKGRLMGLNLEPNYTLHE
                     FPLEAGDRLILFTDGIIETINSQSTFFGKKQLKHFIKKYKDKPASECVHAIIDELKHF
                     AYPNESFNDDITLIIIDIK"
     gene            8769..10091
                     /locus_tag="PLV81_12560"
     CDS             8769..10091
                     /locus_tag="PLV81_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10973.1"
                     /translation="MKYKKIVKRIIFTLLLLFISGQLIIYMYVDIPLSTDFYSSIPKA
                     LVQERQVQYGVKYVLGNGWVHLGWISDPDNEEYTIEIESKSGNREHIGTTQFGSFLYR
                     GNGGLFKVVKINKKTRERFVVGTAKAYSINKKTDIYKPIIAGNYRPLYKPEKHGYYIN
                     DHTIFQDAKRNWRLIGITSKTDGDPNAETYFAVAKSENFPHIMMAEDESVADVGDLAW
                     APHVIKHNNVYHMFWSPHKLHHMISRDGIHWENHAVLMKAPYHKFFRDCMILQVASDQ
                     WLLYTTARGLFFSQVDVYQSFDLVHWQYIRTALRSSFGSERNSPFASMESPFVVKYND
                     GYYLSLTYNNDTLFIHGVLLLFKVWLDKESYNDTLVFYSNNPYDFGVYRGKNRSDSLI
                     ATLRAHAPEWVYVPSTKAWYITTCGWKWVATLTNGEVAIAPVMWQKVK"
     gene            complement(10095..13022)
                     /locus_tag="PLV81_12565"
     CDS             complement(10095..13022)
                     /locus_tag="PLV81_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10974.1"
                     /translation="MKKFFTYSIAIIAGIFVLYLIGYICIPRLLVIGKETKHKIYVSL
                     GFHANFYHSYRIDTNDEAGFGKDIRIVRKIIEVLDEKNKKGIPVKGVWDFENLFTLEE
                     ILPKYAPDIIQNIKRRVKKHGDEVILMSYNNGLVSAMTRDEFVYSMQNAITNTRKSGV
                     KDIFGIFLPYVRPQEMMTSAGNFNLYKELGIQGVVLYYSAIPFDAFRVFVDELSLEEA
                     HNPLIYNNTITNERMLVIPAYNHADLIENVSIYNWVQMLRHEQLRGNIKNDVLICINS
                     DADDSYWYGYKLPSYLKWVPNTGGLSQLIDDIASLDYVEFTTIHDYVQHHKPVKEISF
                     GQDTADGSFNGYVSWAEKKYCSDYWTAVEKDRMAHAFVKALYKYIKQPISEKIQAALV
                     ESFQKRMRLLSTTNFGMATPFLAKNREHVVDDLIKQMQSITDTVIRDLKAYAQKICSQ
                     DKKAQEFSNYEYVNSVLVLKQNADNFVFTISGELPVGYDYFLKYNDTPIPVFVFSQNG
                     ISRFFVKGGIPSDGVYHIVRKPHSQTQYTTHATQQLLKNSTIVIKFKHGINSIEVNEK
                     EVLDEYSFLPWIQYNGKNYRPKTVNIKIINDGTNGVAAVKLYGTLNIPQNITTGNFEY
                     CIYLVEGVDMIFVDGVITYPDTPRTKIFKSSIPQLARIYDPSWQQVAPCPLYVSMHAS
                     KKKPFTVLKRNYLGVESQYTIDYFKHSPKNLDLANVNNHITAEYVAVTNGTECIAVAK
                     ENATLSNFAFCPLQVAHSFFTGFSVSLNPFGTFFGRQYYQPTWGKGRGFEAAIINGDQ
                     YYSGACTYSGTTQRFALAIVHSNNSLPETLKRLMISYANHPFAILMHEKQEKKTTPPL
                     QPPKGVFALYRDSGVYVNFEKADGAASYIIHCGDTKDSLSALYRTKDTSIFVKEYSKG
                     KSFVQGNTYYVAVQAVSAQGKQSELSAMYAFTAKEIKEDMDLPLTLQLKIMFDTLIAK
                     LRW"
     gene            complement(13073..>13263)
                     /locus_tag="PLV81_12570"
     CDS             complement(13073..>13263)
                     /locus_tag="PLV81_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10975.1"
                     /translation="PLPKILLGTTTRSLVSCYLSATDPSSISCCIHQLYLVYYGYCLF
                     YNSYEELLFWVTITQWLG"
BASE COUNT         4196 a         2628 c         2156 g         4283 t
ORIGIN      
        1 aaataattat atatattcta gaaattcctg ccaaaagtgg aataggtaaa tatattttca
       61 taaacaacgc tacttaatct ttcattaatt atttatatcg atagttgttg aaaaaactat
      121 cattgggtga atattttcaa tagatataat aaatactgga ttcatattga catgaattag
      181 atttttatat tcaggaagga atgcacgcca gacaggactc gaacctgtga cccacggctt
      241 agaaggccgt tgctctatcc tgctgagcta ctggcgcgtt aaaattcggg gcgagaggat
      301 ttgaacctcc gacctcatgg tcccaaacca cgcgctctag ccggactgag ccacgccccg
      361 aaataaataa aaattgtatg gaaagaaata tatcacagca aatgattttg tcaaagtaat
      421 aaatcaatac tttgtgtcaa taaaatttta tttgtgattg tgtttatata tttcaacacc
      481 cactctctgt atgtgagact ttatattttc tcctatcagt tcatattttg ttacatcaag
      541 tgtatagggt atttccagtt catctaattg cgcataaagc tttaaatagt gtgaaaaatc
      601 catttgtggt gcaattataa ctatatctat atctgatcct tttgaataag atccttttgc
      661 tcgggaacca tatataagca ctttttctat ttcaggatat tgtaagaata tttctctcag
      721 actgttaata attgtatctt caatgccaaa tgtattttta atcattcatt tttatctcca
      781 taaatctttt aaatagcttt tctaattcag cataatatga ttttacaatc tgtgaaacaa
      841 ttgaatctgc aatcatctga ttatatgtat gtgctgttaa atttcttgtt tttataattt
      901 ccatccagat accaccattt tcaataattc caaatttaaa tgccatttta acagcatctc
      961 ttgaaccctg tatgtcatgt attccctggt ataggaaata gtctctgatt aaattccatg
     1021 ccagttcaaa tgtgtactca aatgcctgaa tcataccctg tttttcaagc tcgtttaatc
     1081 ctttttcatt atattgaacc actgctgctt ttaattgatt gattgctttt tcaaagtttt
     1141 ctaatctttg caactagcga acatcttctt tcatattgct tttatcccaa taacaaaata
     1201 gcgtcacata ttaaatttta taaataataa tccaaatacc ctattactat ttataagaaa
     1261 ttaattaacg taaattacaa tactattagt gtcaacatac aataatagtg tgaattacga
     1321 aatgtacact tttattataa aaataaccat tgaaaatgtc aatggataat agttttacaa
     1381 tgcgattgag tcttatatta ttacaatatc tcaatgaaga aacgtggata ctttctttgg
     1441 cgttaattat tttagaaaag atcaaaatta aaaatttatc ataaccacac acaacaggtt
     1501 atgaataaaa ttaaaatata cttctacttc aatggataat cctgaagtac tttttgaagc
     1561 agtggtacat cgtcggttac tataccgtca acgttgacat caagcagtct ccgcatatct
     1621 cgttcttgat ttaccgtcca cacatcaacc tgtattccac gctcatgtgc catacgtatc
     1681 agtgccccat ttgccagatg tgaaggccca atgtattccg gtatttgcag tgcatcacca
     1741 ggaaaatgtt tgaaaagccc caaaagtcct gacctgaata aaaaataaaa ccccaacaca
     1801 ccccataacc caaatgatgt tgccatggtt gggattcttt tacgtatata ttgtaaattt
     1861 ttaccatgct gcgatgcacc cagaaccctg tgttcagatt tatggctaag aactatatca
     1921 caaaattttt ctgccagcac tatggtatta tctttcaggt caacgttaaa gcgctgttca
     1981 ggaaaagcat ccagcatctc ttccagtgtc ataatagtaa tacctttgcc gcggtatggg
     2041 taagtttctc catccaagct gaaatgatat gctgcatcta attgttttat ctcttgcaat
     2101 gttaactggc caacatttcc cttaccatca gttatgtgcg atagttcatc atcatgaact
     2161 atcaccacat gtccatcccg ggtaaggtgc gcatctgttt caatgacatc tgctcctaac
     2221 gcaattgctt tttcaaacgc cagtgctgtg ttttctggat attcttttga atatcccctg
     2281 tgtgcaaaga tacgggctgg tttttcaaaa aagggctttt tcataattgt actcttttta
     2341 gtatataatc tactgcatac tgtaaattct ttttaactat cacacaatta tcaatttctt
     2401 ttttatgctt tttgccatgc cctgtcaaca catatatacc ctgcatccca gcattttttg
     2461 caagcagtat atcagatgga tgatcaccaa taacaaaaga atgttgtaca tccagattat
     2521 agtgcgctac agcttgatca acaaaatagg tctttggttt tctgcaatga cagttatctt
     2581 cttttgcatg cgggcacata tatatggatt caattgtaat gcctttatca gcaaaaacgc
     2641 tttggatata cgtatttact gtaatagcct catctaaggt tacaattcct tttgcaatac
     2701 caggctggtt ggttatgata aacagcctga aaaactgctg cgcttccttc aaagcatcaa
     2761 acgtacatgt ataaaaaact acacgcccgg gattacttat ataccccaca tcctttatga
     2821 gtgtgccatc tctatcaaga aataatgaag gatatttttg caatgccatt taatcttcca
     2881 ttattaaacc ttcatctttg atgagctgca tatactcttc aattgttcca tcagacaaaa
     2941 aacactcaat gatctgtttg cctaagggaa gaagattggg atcattgtag taatccataa
     3001 gttccttctt gaaaaaatca accagtatct gagctcccct gtcatacgca tcttcaccaa
     3061 cttcactttg aagctcaggc cttaaaagaa acttctcaat ttctactcct tctataacaa
     3121 gcttatttaa tgaaaatccc aacaatgaac aacgtgattt agacaattca tcagcaaaaa
     3181 attttacacc acctcttctt gctaaatatt cacgcataat ccattgtggg ttaaatccaa
     3241 cttgccaggc accaatatac tgattgggtg ccagcacata ttttaccttt ggactggcaa
     3301 ttatctgctc aagtagcaaa tttgcaaaat taactttttt accagttgca aaaggccaga
     3361 aagaaccaac cccttcggat tcaagccctc cagtttttac aatacttgga ttatcatggc
     3421 cacgaggtga cacaagcctc cacaaccagg ctattgccgg tggtaatata tggaataaac
     3481 ctattattcc ataatcaggt ttttcttttg ttgaaggagg agtcctgaca ccaaaacttc
     3541 ttatatgaac ctgaacaggt ttattaacaa tgtccggcaa tgcattgcgt ggaaccagaa
     3601 aacgaggatt gggacatgat ttacccggct catcttcaat gtgttcccat aataaagcag
     3661 tactgaatgg ctgcgcatct atattcaaaa acattaatgg cacctttgga tgtattgaaa
     3721 gcgattcaat atcaggatca gtgccatact gttcaatatg attaacccgt ataaaccatc
     3781 ctgcttcagc atctttaagg tataaataac catcatcttt ttgaaatgat ggatggcata
     3841 atcccatatc atcaacaact ggccgcaaaa aacacccccg tggcagtacc agtgataata
     3901 cttcgccagt cacaatattc acaccaagct ttatggtacc atccacatct cgatgaatat
     3961 gttcattggt ttcactttta ccacttccac tagcaccttc atgcattatg gttgtcttat
     4021 taccatacgg ggtaataacc tgcaccacag cacagtgatt tgttacccaa ccttccttct
     4081 cgccaaaatc aagcaacgat gaatatacac ctttctttgc acttggcccg ggatacagat
     4141 tataactgta tatttcatgc agattttcac tcctatagtg aacaactatc tgtttcccat
     4201 tgaaatgggt atgcctgaaa acaggtgcta tatagagtat acatttaaca tgtatggtgc
     4261 cttcaatttg tcttgaatca atgattccct gtaatatccc caggcctaaa caaaagaaac
     4321 cagcatttgc aggtgctatt gccataccat aacacccatc aagttgccct gctttgaaga
     4381 aaaacactgc taagtcctgc ttttgtaacc aatcaaatgt ttctttttta agttcattaa
     4441 aatctttccc aaatctttgt gtataggtta tcttatcagt tggcaggtca tctccaataa
     4501 ccatgctatc aggatcgcgt ctgcgcatgt atgcttcagt ataattaaca gctatcccat
     4561 tttttacctt ataaacaacg gcctcacagt attccccttt gcctggcaca tcatatacaa
     4621 ctttctgaac accatcattc ccaaatttga gtgataactc aaataaatca tcaactgtat
     4681 gcgctaccgt tacatttgta acctgtaata acgcttttac ctgttctgga acaattaatt
     4741 tactcagcag gtctttcatt gcattccctt tctaattcta aaaaatgtat taactataat
     4801 caccactggt cagttttaaa tacataaaga aaatttcaat attttttcta agagaatttt
     4861 gtatactgat gaaatgtatt acctaatcta atatgttgtc ctattaatta agcacggtaa
     4921 gttgcaattt taaatgcaaa aaaatattgt tgtgatatac tcagtaaaga tattacttat
     4981 taccgaaatc aatagcgtgg tgataatttt tatacaccat actatttgtt tttgcaatta
     5041 aattttaata tcatttagct atttatgctc ggtacattat tttttataca gtatctgata
     5101 tttacagcta tacctccgga taacactcct gagatgatac ttcttcaaga tacaaccaat
     5161 aaaattgata tgaccgacta tctgtactac cttgaagata caaccaaccg tttacaaccc
     5221 aaagacatag tacaacagca aatccaatcc aggtttaaaa aagtacataa aaagataaac
     5281 tacggtttta caaaaagcac attctggatt cgttttaacg ttaaggatat ttcttcatcg
     5341 cccaccacat ggtacctgct ccttgattac cccctcattg aaaatgtaac cctgtataga
     5401 aaaaagagtg gccggttgca aaaacttgca gaaatatctc aacaacaaca attgaataca
     5461 actgatattt tatatacggg acgtatattt gaacttaatc ctggtaaaaa ccaaaatgat
     5521 gaatactata tcaaattaaa aacccgaacc acaatgcgct tccctttgtc aatatgcaat
     5581 ataaactata taaataaaac atccagtttc agaatgcata taaatggaat ctatttaggc
     5641 ataatcttta ttatgttctt ctttaacctc ttttattact ttataatacg gtcatataat
     5701 agcatttttt attcaatgta tattattgga tttggtttgt ttgtttcaat tcaaaatggc
     5761 attttctttg agataattcc gcgttttccc atcaaatatt tattgccact ctatatgggt
     5821 acagggtcac ttgtgttcat attttctaca ttgtttacaa tgttcttttt aaaaacccgc
     5881 actcaactac caaaaatcaa taaaatccta cagggaatta ttgggatttc actggtgttc
     5941 cttgcgctat tacccttcct tgatatgagc tgcatgatac aactttttgc catatttgga
     6001 atactatggt catttgctaa ttttatagca ggtatgtact ccctgtacac aggatacaga
     6061 tctgctcgat tttttatcac gggctggata tttttcttta ttggtgtttt aatctttgca
     6121 ttacgaggat ttggattgct acccagcaat gctataacac tttacagtgt tgaacttgga
     6181 tccatactgg ttatattctt tttatcgata gctatggcag accagtatta tatcatacaa
     6241 caggaacatt cacggcagca atctatcatc atagaaaaag aaaaggcttt gcgcgaaaca
     6301 caagaattgc ttatacaaaa tttacaccag atgagcactt tcaaggatga cctcatcaat
     6361 gcgacatcaa gtcagctccg aaccccactt tcaagtatta ttggcataac cgaatctctt
     6421 ttaggtggaa tagcagggga acttaaccaa aaaataataa ataatctgca acttattctg
     6481 gcaagcagca aacgactgtc acgcttaata cacaacttcc ttgattactc acagttaaaa
     6541 tataatacca cacaattgtc gcttacagct gttgatctta aatcaatgat tgatgtcgta
     6601 attgatatta tacagaaact gccactgtca aattccatta ctataaaaaa taatattcct
     6661 gtaaacttgc cgcttgtgtt ggcggataaa gataggcttc cacaggcact attcaacatc
     6721 ttatacaata gtatcaaatt aacttctaat ggaactatca cattacattc agcaatacaa
     6781 acaaatgacc cttcaaaaat tactatcgcc attcatgaag atggtaaagg ggtgctccca
     6841 tatatctttg acacaattat tcaccaggat atatatctta accagaaaac acctaaaaat
     6901 tctttcttaa ccggtattga attaactatt gcaaaacaac tcattgagct ccacggcact
     6961 actatagcaa ttgatacaaa tcctgacaca ggaacaacgc tgtcattttc tttacaatgt
     7021 gcagattttt cccctgcaca ggatgtccac gcttatcata aagaaacccc cgttgtggat
     7081 aatcacttta tcgatcttgc atctatgcca tatatagaaa acattgataa aacgtcaata
     7141 ccatccactc aacaaattgc ccgtattcta gtggcagata gtgaccctgt attactacaa
     7201 agcattgata acaatcttgt gctgaacgga tatcaggtat tgaaagcata taatggacag
     7261 gaagtattgg gcattcttga ttccggcatg cttcctgacg ccatgcttct tgatattatg
     7321 ctgccaagaa caaatacact ggaagtaatc cgaaagatac gtaccacata cactattgct
     7381 gaattgccaa taattgtgat atcacccatt gaagaaccgg aaagcctgat ggccagcttt
     7441 gaagccggtg caaatgatta tatagtaaaa ccgttttata tgtatgatat catctcacgg
     7501 ttaaaaacac aactgctatt aaaacaggca gtagcaagca tcaaacgggt tactcaaatt
     7561 gaaaaagaat tagatatggc gcgccagatt cagcaaacat taataccaca ggaaactcct
     7621 gtttcgggca aatatgaaat tgcacatgtt tacattccca tggataaagt tggaggggat
     7681 tattacaatt tcaatatcaa agacgataat cacattggca tttttatcag cgatgtatcg
     7741 ggacacggca tgccagctgc tctcattgct tcaatgctac aatcactcat acataccggc
     7801 catcaagctg ttgatgatcc cgtgtccctg ttgcacaaat tgaacacatc attgaaaaaa
     7861 aacctggatg ataattttct taccgcacta tatctatata ttgattttga atcaatgaca
     7921 tgtaaagtat cccgtgcagg acatgagccc tgtatattgt tcagacgctc gcaggatgct
     7981 atatatgaaa ttctgcctaa aggaagatta atgggtctga atcttgaacc caactatact
     8041 cttcatgaat tcccccttga ggcaggcgat cgtcttattc tttttactga tggaataatt
     8101 gaaacaatta attcacaaag cacctttttt ggcaaaaaac agcttaaaca cttcattaaa
     8161 aaatataagg acaagcccgc aagtgaatgt gtacatgcaa taatagatga actaaaacat
     8221 tttgcttacc ccaatgagtc tttcaatgat gatatcacac tgattattat tgatattaaa
     8281 tgagctttct tattgtcaaa atctttggta taatatcatt tttttatttt cttatgcagt
     8341 taattttttt attataccat taaaaatatc catcaaatca agtttgcaat actttcaaat
     8401 taaataactg attatccctg aaataatttt atatataatc actcttttaa acatccccgg
     8461 agacgatagt tagctctaaa ttaaatacgc tttattacat tgctgcagtt tattagccca
     8521 ttaatgtatc actatagcat aattattatg cttgctattt ataaaaatca ttggcaaaat
     8581 ttctttcgaa atatcattaa aaaattggat tattgtattg ttaatcttta ttgtaaagga
     8641 cccgggatgc aacctaaact aaataccagg gaatgggggg ggagaatctt aacatataat
     8701 cgcaataaaa tggggactat ctgtagaatg acatgcgttt ttcatttagt attttaaggg
     8761 gggaaagtgt gaaatacaaa aaaattgtaa aacgcataat cttcacgtta ctattacttt
     8821 tcatttcagg acaacttatt atatatatgt atgtggatat acccctttca actgattttt
     8881 atagttcaat tcccaaagca ttggtacagg agcgtcaggt acagtatggt gtgaaatatg
     8941 ttttgggcaa tggttgggta cacctgggct ggatttctga tcccgataat gaggaatata
     9001 ctattgaaat tgaatcaaag agtggcaacc gggaacacat tggcaccacg caatttggtt
     9061 ccttcctgta taggggtaac ggtggattgt ttaaagtagt aaagattaat aaaaaaacca
     9121 gagaacgttt tgtggtagga actgcaaaag cttactctat caacaaaaag acagatatat
     9181 acaaaccaat cattgcaggt aactatcgcc cattatataa acctgaaaaa cacggctatt
     9241 acataaatga ccataccatt tttcaggatg caaaacgcaa ctggcgcctt attggtatca
     9301 cttcaaaaac tgatggcgat cccaatgccg aaacatattt tgcggttgct aaaagtgaaa
     9361 actttcccca tatcatgatg gctgaagatg aatctgttgc tgatgttggc gacctggcgt
     9421 gggctccgca tgtgatcaaa cacaacaacg tatatcatat gttctggtca cctcataaat
     9481 tgcatcacat gatttcacgc gatggcattc actgggaaaa tcatgctgta ttaatgaaag
     9541 ctccgtatca caaatttttc cgcgattgta tgattttaca ggttgcaagt gatcagtggc
     9601 ttttgtatac aactgctcgt ggactgttct tttcacaggt tgatgtgtat cagtcatttg
     9661 atctggtaca ctggcaatac attcgcacag cactgagaag cagttttggc agcgaacgca
     9721 attcaccatt tgcgtcaatg gaatcgccat ttgtggtgaa gtataatgat ggctattatc
     9781 tgtcactaac gtataataac gatacgctat ttattcacgg tgtgttactg cttttcaagg
     9841 tttggcttga taaggaaagc tacaatgaca cgcttgtgtt ctattctaat aatccctatg
     9901 attttggagt ttacagaggc aaaaatcgtt cggactcact catagcaacg ctgcgtgcac
     9961 atgctcctga atgggtgtat gtgccatcta caaaggcatg gtacattacc acatgcgggt
    10021 ggaaatgggt tgcaacatta acaaatggtg aggtggcgat agctcctgta atgtggcaga
    10081 aggttaagta aacttcacca gcgcagtttt gcaatgagtg tatcaaacat aatctttaac
    10141 tgcaacgtta gaggcaagtc catgtcttcc tttatttctt tagcggtaaa ggcatacatg
    10201 gccgaaagct cagattgttt tccctgtgcg ctcacagcct gtaccgcaac atagtatgta
    10261 ttgccctgaa caaacgattt gccctttgaa tattctttta caaatatcga tgtatctttt
    10321 gttctataca gtgcagacaa tgaatctttt gtatcaccac aatgaatgat gtaggaagca
    10381 gcaccatcag ctttttcaaa atttacatat acaccgctat cgcggtataa tgcaaacact
    10441 ccttttggcg gctgtaatgg tggtgtagtt tttttctcct gcttttcatg catgagtatt
    10501 gcaaatggat gatttgcgta tgaaatcatc agtcttttta atgtttcagg caatgagtta
    10561 ttactatgaa ctatcgccaa tgcaaaacgc tgagtggttc cactgtaagt gcatgcacct
    10621 gagtaatact gatccccatt tatgattgcg gcttcaaacc ccctgccctt accccatgtt
    10681 ggctgatagt actgcctgcc aaaaaaagtt ccaaacggat tgagacttac tgaaaatcct
    10741 gtaaaaaaac tgtgcgccac ctgtaatgga caaaaagcaa agttggaaag agtagcgttt
    10801 tcctttgcaa cagcaataca ctcagtacca ttggtcacag ccacatactc tgctgtaatg
    10861 tgattgttga cattagcaag atcaagattt tttggcgaat gtttgaagta atctatggtg
    10921 tactgtgatt ctacacccag gtaattgcgc tttaatacag taaaaggctt tttcttagat
    10981 gcatgcattg acacatacaa tgggcatgga gcaacctgtt gccagctggg atcatagata
    11041 cgcgcaagct gcggtatgct tgatttaaaa atttttgtgc gaggtgtgtc aggataggtg
    11101 attactccat caacaaaaat catgtcaaca ccttccacca gatagatgca gtattcaaaa
    11161 ttacctgtgg tgatattctg tggaatgttg agggtaccat acagttttac ggctgcaaca
    11221 ccattggtgc catcattaat tatctttatg tttactgttt tggggcgata gtttttacca
    11281 ttgtactgta tccagggaag aaaactatat tcatcaagaa cctctttctc attaacttct
    11341 attgaattaa ttccatgctt gaatttaata acaattgtac tgtttttgag aagctgttga
    11401 gtagcatgtg ttgtgtactg agtttggctg tgaggtttgc gaacaatgtg atacacacca
    11461 tcagaaggaa tccctccctt tacaaaaaaa cggcttattc cattctgtga aaaaacaaac
    11521 acaggtattg gcgtatcatt gtatttcagg aaatagtcgt aacctacagg caattcaccc
    11581 gaaattgtga acacaaaatt atcagcattt tgtttaagaa ccaacacaga attaacatat
    11641 tcgtagttac tgaattcctg tgctttttta tcctggctgc aaatcttttg tgcatatgct
    11701 tttaaatcac gtataacagt atcggttatt gattgcattt gtttaatgag atcatcaaca
    11761 acgtgttcac ggttttttgc cagaaatggt gttgccatac caaagtttgt tgtagaaaga
    11821 agccgcatac gtttttgaaa cgattcaacc aatgcagctt gtatcttttc agaaatcggt
    11881 tgcttgatgt atttatacag cgcttttaca aatgcatgag ccattctgtc tttttcaact
    11941 gcagtccagt aatcgctgca gtatttcttt tcagcccatg atacataacc attaaaactg
    12001 ccatcagcag tatcctgccc aaatgaaatt tcttttacag gcttatgatg ttgcacgtaa
    12061 tcatggattg tagtaaactc aacgtaatca agtgatgcga tatcatcaat gagctgtgat
    12121 agtccgccag tattgggcac ccattttaaa tatgaaggta gcttataccc ataccagtat
    12181 gaatcatcag catcactatt tatgcatatc aatacatcat tttttatatt cccacgtaat
    12241 tgttcatggc gaagcatctg tacccagtta taaatgctta cattttcaat gaggtcggca
    12301 tgattatatg caggtattac aagcattctt tcatttgtta ttgtattatt gtatataagc
    12361 ggattgtgag cttcttcaag cgataattca tcaacaaaaa ctctaaatgc atcaaacgga
    12421 atagcactgt aatatagaac aactccctgt ataccaagct ccttgtatag attaaaattc
    12481 cctgcagatg tcatcatttc ctgtggacga acatagggca aaaaaattcc aaaaatatct
    12541 ttcactccac ttttccttgt attggtaata gcgttttgca ttgaatacac aaattcatct
    12601 cgagtcattg cagaaacaag tccattatta tacgacatca aaatgacttc atcaccatgc
    12661 ttcttcacac gacgtttgat gttctgtatg atatcgggag catactttgg taatatctct
    12721 tccagggtaa atagattttc aaaatcccac acacctttaa caggaatacc ttttttattc
    12781 ttttcatcca gcacttcaat aatcttacgt acaatgcgta tatctttgcc aaaacctgct
    12841 tcatcgttgg tatctattcg gtatgaatga taaaaattgg catgaaatcc caatgacaca
    12901 tatattttat gctttgtctc tttgccaatt actaaaaggc gtggaataca aatataacca
    12961 attaaataga gtacaaatat gcctgcaatg attgcaattg aatatgtaaa aaactttttc
    13021 atatttatat ctcccactga aagcaagttg tagtaatcat aatgctaaat tattatccca
    13081 accactgtgt gatcgtgacc cagaataata attcctcata gctattataa aataaacaat
    13141 atccgtagta taccagataa agctggtgaa tgcaacaact tattgatgat gggtctgtag
    13201 cacttagata gcatgataca aggcttctgg tggtggtccc cagtagaatt ttaggcaatg
    13261 gat
//
LOCUS       DAORYL010000083        13196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_3681_length_13196_cov_13.529336, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000083 DAORYL010000000
VERSION     DAORYL010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13196
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3681_length_13196_cov_13.529336"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..661)
                     /locus_tag="PLV81_12575"
     CDS             complement(2..661)
                     /locus_tag="PLV81_12575"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOM10976.1"
                     /translation="MKRYTLPQLPYEYNALEPYIDAKTMEIHHTKHHATYIDKLNAAL
                     DKYPHLYELSLEHLLANLATLPEDIRTVVRNNGGGHYNHSLFWKILGGRGELPGGKLL
                     EAIKNKFGTVETFIDEFSNAALNRFGSGWAWLSVDALGELVVHSTANQDSPVMEGLLP
                     VLGLDVWEHAYYLSYQNRRADYIKAFWNIVNWKQAEENYKKAIEAVEHCSSHPECREV
                     A"
     gene            complement(962..1510)
                     /locus_tag="PLV81_12580"
     CDS             complement(962..1510)
                     /locus_tag="PLV81_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10977.1"
                     /translation="MLRKKIKYLTTLINNYYFDENNNLVIQYQMIIAIIGSIVLMVGV
                     FLPFIKAPFLGVVDYTRIGKYYDEIVIACGLVAIILALLKLYRWLVIPAFGSLCVIVF
                     SIYKVFSKVSMLREMVEDKLYNNPYKDLIATFIDSMQEATSLQWGVIILGTGVLLLLL
                     AGMLQTKNIVIPKKVNSPQSNM"
     gene            2073..3056
                     /gene="selD"
                     /locus_tag="PLV81_12585"
     CDS             2073..3056
                     /gene="selD"
                     /locus_tag="PLV81_12585"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOM10978.1"
                     /translation="MGPADLAQVMKKLPIEYNSDVLVGMQTSDDAGVYRISDDVALVQ
                     TVDFITPIVDDPFVFGQIAATNSISDVYAMGATPITAMNIVCFPITTFAMDVLEKILQ
                     GGLDVMKKTGVQLIGGHSIDDIELKYGLSVTGITHPNTIFTNTGLQPDDVIILTKPIG
                     TGTINTAIKAGIAANEHIERAIQSMTTVNDCLSGYPHREHIHAVTDVTGFGLIGHLSE
                     MLGTGSIAIHVHANDVPLIPGATDYAAMGLLPAGLYRNRDFVGSRCSVASGVKRDIAD
                     LLFDPQTSGGLLIATSKHTAHQVIEYIAAKALCTAIISTCSFSQEGHIYIE"
     gene            3077..3448
                     /locus_tag="PLV81_12590"
     CDS             3077..3448
                     /locus_tag="PLV81_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10979.1"
                     /translation="MKYSDDKRQQLLSKVIWDYNLDIKEIIKTIDNDTITHNKKQIFV
                     RCLQRLPWHNVVGIFGFDAAKTLLTDDVIEQIWPKERREHFATLQKLLRGEPLPSARW
                     DSELREKLKSTVLSYRWYRFQ"
     gene            3534..3626
                     /locus_tag="PLV81_12595"
     tRNA            3534..3626
                     /locus_tag="PLV81_12595"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3568..3570,aa:Sec,seq:tca)
     gene            3648..4982
                     /gene="selA"
                     /locus_tag="PLV81_12600"
     CDS             3648..4982
                     /gene="selA"
                     /locus_tag="PLV81_12600"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOM10980.1"
                     /translation="MYEQIPQVQKLLEEDSIRQFIGEIGHPFVVAVIRETLEEQRKLI
                     AKGSPFDYGKCVEAIAHRCMAIRMEKLQRVINGTGILLHTNLGRAPLGKDILSSVTEN
                     LSAYCNVEFHLPTQKRGKRGGFAEKLICLITGAEDALIVNNNAASVFLILHHFARNKE
                     VIISRGELIQIGGGFRIPDIMKQAGCHLVEVGTTNITDVSDYKNAMTENTALILSAHR
                     SNFYMRGFTQNPTLQQLATLKSDRVLLVKDLGSGNLIFNNDDNVTQALNYADLVCFSG
                     DKLLGACQAGIIAGKKDLIATLKKDPLMRIVRVDKVTYFILQQVLLHYANNKHGVIPA
                     WSFMKQDLHAIDKKIKKLFKKLKHEVKPYIQRINSKTAFGGGAMPDKVLPDYGIAIAI
                     DGKKASDIATHMVSQPVPIIGYIQNDVFTINFRTILDDDIGNVADAINSLQP"
     gene            4992..>6372
                     /gene="selB"
                     /locus_tag="PLV81_12605"
     CDS             4992..>6372
                     /gene="selB"
                     /locus_tag="PLV81_12605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOM10981.1"
                     /translation="MYVIGTAGHVDHGKTSLIKALTGIDADRLPEEKQRQMTIDIGFA
                     FIKLPTIGTVSIVDVPGHERFIRNMVTGAWGIDIGLLVIAADDGWMKQTDDHVKVLSI
                     LGIPSIVIALTKIDKVAPEDITRVLSHIHNKLITTPYRNANVIPCSSITGQGIDTLKA
                     ALEDAIHRLPQPEAGNKPYLHIDRVFTVKGVGTVVTGTSKNGSFMVNDSITILPHTIT
                     TRIRQIQSHNTQQEKSDVSQRTALALAGVEKATLQRGHIIVKENFFTETRSCTALITH
                     TFSPIKNNQWIEVLIGTAACDAQYSVIGQSNTVVRLKFKEPVYCYPTERFVATFPGGY
                     TIIAGGYILSTDYKGAIKKDPLLQKLSQSNDTTANAILCTLITNPYMSIEHLKKIFPD
                     EAHVVDTIERLKSNSQVTIIGNYISTTSHYDTTVNIVKQECASDITEQELAQKLLLPY
                     DVVHHIVTHH"
     assembly_gap    6373..6472
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6662..7696
                     /locus_tag="PLV81_12610"
     CDS             6662..7696
                     /locus_tag="PLV81_12610"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOM10982.1"
                     /translation="MALFTPNVDKLREKNDIKGLLQLTKHRKAEVRLNALLALVKIRD
                     QSVIEDLKKLIHDPDPRVRTIATIKFGNIKDPVIIENLRQIIISGSQRDKIEALRVLS
                     EIGPTDDLEISKILYLAFKDKKMMVSIEAIRTMGYLKDKYAIPHLVEALDSKHYQIRV
                     EATRALGMIGGDQAVSPLIGCLVDNNAEVRKTAHDALKKIGTERALKAITDAPFMLLV
                     KRMTEGGAQREETVKQIGQFKIKEAAPLLIKACNDEFKSVRMSAARSIGILREKSAIG
                     ALVKLLDDPYYDVRLEAVRALEKIFDPQALKAIEKAMSDPNKNVRDEARVCYYSLKSR
                     LERVHDTEQV"
     gene            7703..8467
                     /locus_tag="PLV81_12615"
     CDS             7703..8467
                     /locus_tag="PLV81_12615"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOM10983.1"
                     /translation="MIIGFNIGNTHTVVGLYEAGNPLPVFMDRYPTKREVTVQELSDT
                     ITSAINKYSVDAGAMQGAIYSSVVPQCNDAYDAVCKKLFSINPHRITYRSRMPITIVY
                     DNPAQLGVDRIVNAVAAHIDYNTDCIIVDIGTAITFCLLEHGVLKGGIIAPGVKAAMK
                     GLAQSTAQLFEVPFEKPASIIAQNTKDALISGFFYGWISLIEGIIQKMAGAIAHYTVI
                     CTGGMAEVLQPYLSIPANIDTTLTMRGLYYLYDINR"
     gene            complement(9067..9249)
                     /locus_tag="PLV81_12620"
     CDS             complement(9067..9249)
                     /locus_tag="PLV81_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10984.1"
                     /translation="MENTNRLYQYLLQKKKSFAYVSDEGIDGITIMIHARPGKSKGWK
                     SSYEVLQGKLNGALQT"
     gene            9555..9740
                     /locus_tag="PLV81_12625"
     CDS             9555..9740
                     /locus_tag="PLV81_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10985.1"
                     /translation="MVAEFIEARTQHITPYFLYGYHCPLLLRLDLAYAFGCAFSSASY
                     SLYPCGGFRFISLLIVE"
     gene            10323..10784
                     /locus_tag="PLV81_12630"
     CDS             10323..10784
                     /locus_tag="PLV81_12630"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOM10986.1"
                     /translation="MNNYERYTRILARSVDHIFKNFLHDPDIKEVFESQANQNDPKVT
                     IELDGTMKGELIINFPESTLQQLTKNFFGNTRTNVKKYYPDVAGEIANLITGTFINQL
                     QFIKHNLKPSPPEYNEEQIPLKTLYENINLSFQSRFGGFDIDFYYKDFLHR"
     gene            10784..12511
                     /locus_tag="PLV81_12635"
     CDS             10784..12511
                     /locus_tag="PLV81_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HOM10987.1"
                     /translation="MSTIDFIIVAAYLLFSMFIAIMYAKRARTSTQQFFLSGRNLPWW
                     IAGTAMVATTFAADTPLAVTELVRSGGIAGNWLWWNMVAANIVTVFFFAKLWRRAGVT
                     TDIEFIELRYSGKEASFLRGFKAVYLGLFANCIIMGWVNVALAQILAYMFGIHQTYVF
                     FIVIATMIFVGLYAAVGGLWAVAVTDVVQFIIAMGGCIVLAYVVISHPPVNGIEGLKQ
                     QLSPHTLSFIPFGDSASIPLAAFLLYVCVQWWASWYPGAEPGGGGYVAQRMMSSKDEQ
                     HSLKATLWFTIAHFTVRPWPWIIVALATIVLYPAVPADQSKATYIFAIRDFLPHGLKG
                     LLVAAFLAAYMSTISTHLNWGSSYLVNDLYRRFIAPQKSERHYVVIAQVCTIALVIIS
                     SALIFVIDSISSAWAFIIECGAGIGPVLMLRWYWWRINAWSEITAMIAPIPAVIVSRV
                     LLGIAFPYNLLIIVPFTTLCWLAVTLLTNPVDNDKLLSFYKTVTPEFGFTPLRKAMQH
                     PANNKLLVVRIAQTLLGIIATYSLLFGIGAFILHNDYALTSITVFAVSFALILYLLSF
                     ENKLYADKR"
     gene            complement(12432..12782)
                     /locus_tag="PLV81_12640"
     CDS             complement(12432..12782)
                     /locus_tag="PLV81_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10988.1"
                     /translation="MALDFLIFSMDKRDFIDEIQFVLVEIVIALIIIGLLTFVIIRAI
                     ARSFSNPIQNTVVILKRLAEGDLAAESNDFLADERCVYHSTTIIHVFIFYLHTIYFQK
                     KEDTVLMQNLLQIP"
BASE COUNT         4059 a         2601 c         2486 g         3950 t
ORIGIN      
        1 attacgcaac ttctctgcat tccggatgtg aactgcagtg ttcaacagct tctatagcct
       61 ttttataatt ttcttcagcc tgcttccagt taacaatatt ccagaaagcc ttgatatagt
      121 ctgctcttct gttctgataa ctaaggtaat aggcatgctc ccacacatca agtccaagca
      181 ctggcagcaa tccttccatg acagggctat cctgattggc agtagaatgt actaccagtt
      241 ctccaagcgc atcgacactg agccatgccc acccggaacc aaaacggttt aaagcagcat
      301 tgctgaattc atcaataaat gtttcaacag ttccaaactt gttttttatt gcttccagta
      361 gcttcccacc aggcaactcc cctctgccac ctaagatctt ccagaacaat gagtggttgt
      421 aatggccacc accattgttg cgcaccactg ttcgtatatc ttcaggcagc gtagctaaat
      481 ttgccagtag atgctccagt gatagctcat acagatgagg atatttatcc agtgctgcat
      541 tgagcttgtc aatgtatgtt gcatggtgtt tggtgtggtg aatttccatg gtttttgcat
      601 caatataagg ttcaagagcg ttgtattcat atggcaattg tggtaatgta tatcgtttca
      661 tggtttttct ccttttcaag atttgtagta ttaactaaat tattgtaata tactcattat
      721 ttttgttttc gtcaacaggt aaaaaacatt ttgggcgata gttactgtaa aaaagccttt
      781 taacaattac tcaactttta ttaatatatg aattttcatg aactatcgca taaaaaatat
      841 aaaggcaata ctacttaaaa ctttttatac aacgctaaaa atatatgatg gcctgtatca
      901 tgaaattttg aatgaacctg aaaaggtttt ggtaataggt gttatagtgt actggttaga
      961 ttcacatatt tgattgtggg ctatttactt tttttggtat aactatattt ttagtttgca
     1021 acattccagc caatagtaaa agcaacacac ctgttcccag tataattacg ccccactgca
     1081 atgatgtagc ttcctgcatt gaatctataa atgtagctat gagatcctta tagggattat
     1141 tatataattt atcttcaacc atctcacgga gcatgctaac cttactgaat actttgtata
     1201 ttgaaaatac aataacacag agcgatccaa aagcaggaat gaccaaccat cgatatagtt
     1261 tgagcaatgc aagtatgata gcaaccaaac cacatgcaat aactatctca tcataatatt
     1321 tcccaatccg tgtatagtca acaacaccta aaaacggtgc ttttatgaaa ggaagaaaaa
     1381 cacccaccat taaaacgatt gacccaataa ttgcaattat catttgatac tgaatgacta
     1441 aattattgtt ctcatcaaaa tagtaattat taatcagtgt tgttaaatat tttatttttt
     1501 tccgtaacat aacattaaaa acttcctcac cttaatactt ttttattttt tataaaaata
     1561 tcttatatat taatatagca atcatgcacc gacaatacat cacaaagcat atcaaatatg
     1621 tctaacgaag ctttgtattt ttactagaat actatacgtt ctaccagata attatattta
     1681 aggagcttaa tgctagaggc atactgtaat tatgaagaag agtattgcaa gcacaaaatg
     1741 ttcactcaat cgatttggac aaataatgcg gcgaaataac aatatgtaat gacgagagta
     1801 gcatgtcata aacagagcta acagttgaat aaaaaagtta attgaatagt aaataatttc
     1861 atatatctga taggatcata catcaaattg aacttgactc tgaaaaaaat accttttttt
     1921 cttgaaagat attatttatt ctgtcacaat acattatcaa ttttttatat tcaattaaaa
     1981 gattaattac aatgtcaagc tgtgatatca acaaaccaaa gaaagatgtg cgtcttactg
     2041 agtctatacg cggttcagga tgagcggcaa agataggtcc ggcggacctt gctcaagtca
     2101 tgaaaaagct gcccattgaa tacaacagcg atgtactggt gggcatgcaa accagcgatg
     2161 atgccggtgt gtacagaatc agcgacgatg tagcacttgt ccagactgtc gattttatta
     2221 ccccgatagt ggatgatcct tttgtctttg gccagattgc tgccaccaat agcatctctg
     2281 atgtttatgc tatgggcgcc acacccatta ctgccatgaa tattgtgtgc tttcccatta
     2341 ctacatttgc tatggatgta ctggaaaaga ttttgcaggg tgggttagac gtaatgaaga
     2401 aaacaggtgt gcagcttatt ggcggacaca gtattgacga tattgagctt aaatatgggc
     2461 tttcggtaac tggcattacg catcccaata caatttttac caacacagga ttacagcctg
     2521 atgacgttat cattcttacc aaacctattg gcactggaac cattaatact gctataaaag
     2581 ctggcattgc tgccaatgaa cacattgaaa gagccataca atccatgact acagtaaatg
     2641 attgcctgtc aggctacccg catagagagc atattcatgc agttactgat gtcaccggct
     2701 ttggccttat tgggcatcta tctgaaatgt tgggcacagg ttcaattgcc atccatgtac
     2761 acgctaacga tgtgccactc attccaggtg caaccgacta tgctgccatg gggttacttc
     2821 ctgctggatt ataccgcaac agggattttg taggttcacg ctgttcggta gcttctggtg
     2881 tcaaacgtga tattgctgac cttttgtttg acccacaaac gtccggtggg ctactcattg
     2941 caacatctaa acatacagca catcaggtaa ttgagtatat tgcagcaaaa gcgttatgca
     3001 cagcaattat ttcaacctgt tcattttcac aggaaggtca tatatacatt gagtaatgta
     3061 ccggtaacta tcgcccatga aatattcaga tgataaacga caacagttac tttcaaaggt
     3121 aatctgggat tacaatcttg atatcaaaga gataattaaa actattgaca atgataccat
     3181 aacacataat aaaaagcaga tatttgtacg gtgcttacag agacttccat ggcataatgt
     3241 tgtggggatt tttgggtttg atgcagccaa gactttatta accgatgatg taattgagca
     3301 aatatggcca aaggaaagga gagagcattt tgcaacactt caaaagttat tacgagggga
     3361 gcctttaccc tctgcaagat gggattctga actgcgtgaa aaacttaaat ctaccgtttt
     3421 atcttaccgg tggtaccgct ttcagtaggt aatgacaatt ccctctgtac ataaaaaatc
     3481 ctttacaatt attgcccata ctttgctata ataaaccaca tagtgtgtaa aaaggaagcg
     3541 gatgagatct ggtggtctct gcggtcttca aaaccgttgg ctgcctctaa aaggcagtgg
     3601 caggttcgat tcctgcccct tccgcatttt ttttaaagga taacgcaatg tacgaacaga
     3661 taccacaggt acaaaaactg ctggaagaag attctatacg gcaatttatt ggggagatag
     3721 ggcatccgtt tgttgttgcc gttatccgtg aaacgctgga agaacaaaga aaactgattg
     3781 ctaaagggtc gccatttgat tacgggaagt gtgtggaggc gatagctcat agatgcatgg
     3841 ctatccgtat ggaaaaactg caacgtgtga taaatggcac tggcatcctg cttcacacca
     3901 acctggggcg ggcaccactt ggcaaggata tattatccag tgttaccgaa aatttgagtg
     3961 cctactgcaa cgtagaattt cacttaccaa cacaaaaacg gggaaagcgt ggcggttttg
     4021 ctgaaaagct tatttgcctt attaccggtg ccgaggatgc actcattgtc aataataatg
     4081 ctgcaagcgt atttttaata ctgcaccatt ttgcccgtaa taaagaagtt atcatttcgc
     4141 gtggcgagtt aatacagatt ggcggtgggt tccgcatccc tgatatcatg aagcaggccg
     4201 gatgtcatct tgttgaggtt ggcacaacca atattactga cgtttctgac tacaaaaatg
     4261 caatgacgga aaacacagcg cttatccttt cggctcatcg atcaaatttt tacatgagag
     4321 gatttactca aaaccccacc ctgcaacagt tagccacctt gaaatcagac agggtgttac
     4381 tggttaaaga tttaggaagt ggcaacctta ttttcaataa tgatgacaat gtaacacagg
     4441 cacttaacta tgctgattta gtgtgtttta gcggcgataa actacttggt gcatgtcagg
     4501 ctggcatcat tgcaggcaaa aaggatttaa ttgcaacatt aaaaaaagat cctctgatgc
     4561 gaattgttcg cgtggataaa gtaacctact ttattttaca gcaggtatta ttgcattatg
     4621 ccaataacaa gcacggggtt attccagcat ggtcatttat gaagcaggat ttacatgcaa
     4681 tagataaaaa aataaaaaaa cttttcaaaa aactaaaaca tgaggttaaa ccatacattc
     4741 agcgtatcaa ctcaaaaacc gcatttggcg gcggcgccat gcccgataaa gtactgcccg
     4801 attatggcat agcgattgct atagatggga aaaaagcaag tgacattgca acacacatgg
     4861 tttctcaacc tgttcctatt attggttata ttcaaaatga tgtattcacc ataaatttca
     4921 gaaccatact ggatgatgat attggcaatg ttgctgatgc aataaattct ttacaaccat
     4981 aggaaataac catgtacgtt atcggcactg caggccacgt tgatcacggc aagacatcgc
     5041 tcatcaaggc acttaccggt atagacgcag atagactgcc ggaagaaaaa cagcgccaga
     5101 tgaccattga tattggtttt gcctttatta aacttcccac tattggcacc gtgagtattg
     5161 ttgatgtacc ggggcacgaa cggtttatac gaaatatggt aaccggtgcg tggggcattg
     5221 atattgggct attagtgatt gctgccgatg acggttggat gaaacaaacc gatgaccatg
     5281 tcaaagtgct gtcaatactg gggatacctt ccattgttat tgcattaacc aagattgata
     5341 aagtagcacc tgaggacatt acccgggtac tatcgcacat acacaataag ctaatcacaa
     5401 caccatatag aaacgcaaac gttataccgt gctcatcaat aacaggacaa ggtattgaca
     5461 cattaaaagc agcactggaa gatgccatcc acaggctccc ccaacctgaa gctggcaata
     5521 aaccctattt acatattgat agggtcttta ccgttaaagg cgttggtacg gtagtaaccg
     5581 gtaccagcaa aaatggtagc tttatggtaa atgattctat aaccattctg ccacatacca
     5641 tcaccacacg catccgccag atacagagcc ataataccca gcaggaaaaa tcggatgttt
     5701 cacagcgaac ggcactggca ctggcaggag ttgaaaaagc aacactacaa cgtggccaca
     5761 tcatagttaa ggaaaacttt ttcactgaaa ccagaagctg cactgccttg ataacgcata
     5821 cattttcacc aattaaaaac aatcagtgga ttgaggtttt gattggaact gctgcctgtg
     5881 atgctcagta cagtgtcatt ggtcagtcaa acactgttgt gcgccttaag tttaaagaac
     5941 cagtttactg ctaccccact gagcgatttg ttgcaacatt tccaggagga tataccatca
     6001 tagctggagg ttatatcctg agtacagatt ataaaggtgc aattaaaaaa gaccctctac
     6061 ttcaaaaact ttcacaaagc aatgatacaa cagccaatgc tatcctgtgt actcttatca
     6121 ccaatccgta tatgtcaatt gaacatctaa aaaaaatatt tcctgatgaa gcccacgttg
     6181 ttgacacaat agaaaggctt aaaagcaact cacaagtcac tattattggc aattatattt
     6241 ccacaacatc acactatgat actacggtaa atatagtaaa gcaggaatgt gcatcagaca
     6301 taactgagca ggaactggca caaaagcttt tgctgccata cgacgttgtg caccatatag
     6361 tgacacatca tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaatatat
     6481 gataccgctt ttgaatgcca tggagcagaa aaatctcata aagcgatatg gcgatgttcg
     6541 tatcaaggtt tagccgtata tagatttgca taatacaata aaattgtaca ttattagttg
     6601 acttttttta tgcatatgga gtacacttgt aataaatata catacttaaa aagggattgc
     6661 tatggcactt tttaccccaa acgttgacaa actcagagaa aaaaatgaca ttaaagggtt
     6721 actacaatta accaaacacc gtaaagccga ggtccggctc aatgctttgc ttgctctggt
     6781 aaaaatacgt gaccagtcag ttattgagga tttaaaaaaa cttatccatg acccggaccc
     6841 acgggtaagg acaatagcaa caataaaatt tggcaatata aaagaccctg ttattattga
     6901 aaatctgcgt cagataatca tatcaggttc acagcgtgat aagattgaag cgctgagagt
     6961 tttgagtgaa ataggcccca ccgatgatct ggagatatca aaaatactgt atttagcatt
     7021 taaagacaaa aagatgatgg tttcaattga agccatccgc accatgggct atcttaagga
     7081 taaatatgcc ataccgcatt tagttgaggc tcttgacagc aagcattatc agatacgcgt
     7141 tgaagcaacc cgcgcactgg gcatgattgg cggcgatcag gctgtcagtc cattaattgg
     7201 ctgccttgtt gataacaatg ctgaagtaag aaaaactgct cacgatgcac tgaagaaaat
     7261 tggaaccgaa agggcactca aagccattac cgatgcaccg tttatgctcc tggtaaagcg
     7321 tatgaccgaa ggaggtgccc agcgcgagga aacagttaaa cagattggcc agtttaaaat
     7381 caaagaagca gcaccactgc ttatcaaggc ttgcaatgac gaattcaaat cggtacgcat
     7441 gtcagctgcg cgttccattg gcatattacg agaaaaaagc gcaattggcg ctttagtcaa
     7501 gcttttagat gacccttact atgatgtgcg cttagaagcg gtgcgtgcac ttgaaaagat
     7561 atttgacccg caggcgctta aagccataga aaaagctatg agtgatccca acaaaaatgt
     7621 gcgcgatgaa gcccgcgtgt gttactactc gcttaaatcc cgcctggaga gggtacacga
     7681 caccgaacag gtatagcatt tcatgattat aggtttcaat attggcaata cacataccgt
     7741 ggtagggctt tatgaggcag gcaaccccct gcctgttttc atggaccgct atcccacaaa
     7801 acgggaggtt actgtacagg aactatcgga caccataacc agtgcaataa acaaatattc
     7861 tgtagatgct ggtgccatgc agggtgctat ttattcaagt gttgtcccac aatgtaatga
     7921 tgcctatgat gcagtatgta aaaagctatt ttctataaat ccccacagga taacctaccg
     7981 ttcacgtatg ccaataacaa tagtatatga taacccggcc caattaggtg ttgatcgcat
     8041 tgtcaatgcc gttgctgccc atattgatta caacaccgat tgcattattg ttgatattgg
     8101 gactgccata acattctgcc tgcttgaaca tggtgtatta aaagggggaa tcatagcacc
     8161 cggggtaaag gcagcaatga aaggcctggc acagagcacc gcacagcttt ttgaggttcc
     8221 gtttgaaaaa ccagcttcta ttattgcaca aaatacaaaa gatgccctta tttcaggatt
     8281 tttttacgga tggatttctt taattgaagg gattatacaa aaaatggcag gtgcgatagc
     8341 tcattatact gttatctgca ctggaggaat ggctgaagtg ttacaaccct atctttcaat
     8401 ccctgcaaac attgacacaa cacttaccat gagagggtta tattacctgt acgacatcaa
     8461 caggtaaaat gttgaatacc ccaaaaaaca gtctatttat attaatattc acaaagttat
     8521 ccacattatc cacaaacgca gcaggcaatg acattaattt actaaattaa ttggctatcg
     8581 attgaatgta tgttactatg tcgcattgaa ctgcatcgaa tgcagtaaat ttattgaata
     8641 aagccatgct aaatgctgaa aattccagga tttatgttgc aacatttttt aaaaaaaatt
     8701 ttttttactt ttttaattat ttttatgtat aggctgaaag tgtatatatt caaaaattaa
     8761 tttctgttaa aattcattat aatgtggata aaagcttgaa taaaaattgc attatcataa
     8821 ataaaaatat gttgtatttc caaacattgg ttaatgaacg ttcacatatt ttttattagt
     8881 ttttcgcaaa caatatcaaa ccagaataat cctgggtata tcataaagaa gtcatattgg
     8941 cctatgaagt tcaatgaccc tttttatata tcacccattt aaatttggtt agaattaaaa
     9001 aaataatgtc ttttctatat cttgtatcat taccccatcc agtaatctat acagaatgat
     9061 gcagattcac gtttgaagtg caccattgag ttttccctgt aaaacctcat acgaagattt
     9121 ccatccttta gattttcctg gacgtgcatg tatcattata gttataccat ctattccctc
     9181 atcgcttaca taagcaaaac tttttttctt ttgcaataaa tattgatata acctgttggt
     9241 gttttccaca acactccgtt gccaggcaca atacggatct gcacaataaa tattacagtc
     9301 cagtatttcc tctcattttt tgtgataggt acattctgtc ccagtatcaa acggtatggt
     9361 tttttcaatt tttcctgtta aactttagat cactatctct atcccacgca taacggtgtc
     9421 tgcagtctat gaaactttag agccttcccc tgcactttct tttgtccaga cggcaacaaa
     9481 agaaagtgcc aaagaaaaat gcctttacct tgttgacaaa cacattggct atcggttggt
     9541 gagcctgtcg aaccatggtt gctgagttta tcgaagcacg cacgcaacat attacgccgt
     9601 acttcctgta cggctatcat tgccccttac ttttacgcct tgatctggct tatgcctttg
     9661 ggtgtgcttt ttcttctgct tcatactcat tatacccatg tggtggattc cgctttattt
     9721 ctctgcttat tgttgaatga tgcctccctc atctacacga tattcctgtg ctataaagca
     9781 tttttttgat gacaattctt tctccgcatt gtatatgagt ataatgcctc atagctcctt
     9841 tttagttttt gttgttgaaa catgcattct aagggatgtg ttttatatga ggctacatgt
     9901 tttctatttt gagttgcact ttattcttga atttgcacat tttataccac attatactgt
     9961 ggaccacacc ccgaaatagt acaaaaaaca acattactat aaacatttct tgacaaataa
    10021 ccatatttta aattttattt tatagcgcta tttatccctg aactttagat tatttatata
    10081 atcgtaagtt attttttttt actttgagtt gtgaaaaagg ctgtgttaat aaaatttttt
    10141 ttcacgatag tggtttattg tatcacaaaa ggttgcaaaa tcgtgtttga atgaaatcat
    10201 tattgaacac agcacttaca aaatttgttt agcaaacgtt gtttggaagt tctgtcttca
    10261 atactttaaa aagtttgtta taattacatt ataacgtcat ctttgcgggg agaagtattt
    10321 gcatgaacaa ttatgaacgc tatacccgaa tattagcccg ttcggttgac catattttta
    10381 aaaattttct acatgatccg gatattaaag aggtatttga aagccaggca aatcaaaacg
    10441 acccaaaggt aaccattgaa cttgatggta ccatgaaagg ggaactcatt ataaacttcc
    10501 cggaatccac actacaacaa cttacaaaaa acttctttgg aaataccagg accaatgtaa
    10561 agaaatatta tcccgacgtt gcgggtgaaa tagcaaatct cattacggga acctttatca
    10621 atcaactgca gtttataaag cacaacctta aaccatcacc acctgaatat aatgaagaac
    10681 aaataccttt gaaaacactg tatgaaaaca taaacctgtc atttcagtcg cgctttggtg
    10741 gctttgatat tgatttttat tacaaggatt ttctgcatcg ctaatgtcaa caattgattt
    10801 cattatcgtt gctgcgtatc ttttattcag catgtttatt gccatcatgt atgcaaagcg
    10861 tgcacgcacc agcacacagc agtttttcct ttctggtcga aatcttccct ggtggatagc
    10921 tggcactgcc atggtagcaa caacctttgc agccgacacc cctctggctg tcaccgagct
    10981 tgtacgcagt ggcggtatag caggtaactg gctgtggtgg aatatggttg ctgcaaatat
    11041 tgtcacggta ttcttctttg caaaactatg gcggcgggct ggcgtaacca ccgatattga
    11101 gtttattgaa ctgcgctaca gtggcaaaga ggcatcattt ttacgtggct ttaaagcagt
    11161 gtatctgggg ttatttgcca actgcatcat catgggatgg gtcaatgtag cactggcgca
    11221 gatactggca tacatgtttg gcattcatca aacgtatgtg ttttttattg tcattgcaac
    11281 catgattttt gtaggactgt atgcggctgt tggcggtttg tgggcagtag cagtcaccga
    11341 tgttgtgcaa ttcatcattg ccatgggcgg atgtattgtg cttgcctatg ttgtcatttc
    11401 ccacccaccc gttaacggca ttgaaggcct taaacagcaa ctatcgccac atacgctttc
    11461 atttattccg tttggcgata gcgcttcgat tccactggcc gcatttttac tgtatgtatg
    11521 cgttcagtgg tgggcatcct ggtatccagg agcagagcct ggtggcggcg gctatgttgc
    11581 acagcgcatg atgtcctcaa aagacgaaca gcattcactc aaagcaacct tatggtttac
    11641 gatagctcat ttcacagtgc gtccctggcc gtggattatt gttgcccttg caaccattgt
    11701 attgtaccct gcagtgcctg ccgaccagtc aaaagcaacg tatatatttg ccatccgtga
    11761 ttttttgcct cacgggttaa aggggctact ggttgcagca ttcctggcag cgtacatgtc
    11821 aactatctcc acacacttaa actggggcag ttcatatctg gttaatgacc tgtaccgacg
    11881 ttttattgcc ccacaaaaaa gtgaacggca ttatgtggtt attgctcaag tgtgcaccat
    11941 agccctggtg attatctcaa gtgcacttat atttgttatt gattccatat cttcagcatg
    12001 ggcattcatc atagagtgcg gtgcaggcat tgggcctgta ctcatgctgc gctggtactg
    12061 gtggcgcatc aatgcatggt cggagattac agctatgatt gctcccatac ctgcagtcat
    12121 cgtatcacga gtactgctgg gtattgcttt tccctacaat cttttaatca ttgttccctt
    12181 cacaacactg tgctggttag cagtaacatt gttaacaaat cctgttgata atgacaaact
    12241 gctatcattt tacaaaaccg ttacaccaga atttggtttt acgccgttac gaaaggccat
    12301 gcagcaccct gcaaacaaca agcttttagt ggtgcgtata gcgcagacac ttctgggtat
    12361 tattgcaaca tactcactgt tatttggcat tggtgctttc atattgcata acgattatgc
    12421 gttaaccagt attacggtat ttgcagtaag ttttgcatta atactgtatc ttctttcttt
    12481 tgaaaataaa ttgtatgcag ataaaagatg aatacatgga tgatagtagt ggaatgatat
    12541 acacatcgtt catcagcaag aaaatcgttg ctttctgcag caagatctcc ctctgcaagc
    12601 ctttttaata taactactgt attttgaatt ggatttgaaa aactacgagc aatagctctg
    12661 atgatgacaa aggtcaacaa acctataata atcagagcta taacaatctc cacaaggaca
    12721 aactgtatct catcaataaa atctctttta tccattgaaa aaataagaaa atccagagcc
    12781 atatcaatat ctgtgcaatc tttttagcag gctacctgat gcagatagcg atgaagaact
    12841 gtatcagctt ttacctcaca aaataacgat gtaatataca tacgcaattg taaagataat
    12901 tcagacataa taatggattc aaatttttga tttgataaca ttatgtgaac actggtatca
    12961 tcccatttat atttcatata aacatatatg tttgtggtgt ctttatttat agttcaataa
    13021 tattttgaaa tccctaaagt aaagtatgaa aataattttt tcctgagttt tttaaaatta
    13081 tacagcatta ttctacctgt gattaatttg acacttacga aaaattagag gaaaaaaatt
    13141 aattaaataa ggcttaaaat aagtaaaaag gaaagcacgg tcattgattt tgaaat
//
LOCUS       DAORYL010000084        12875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_3816_length_12875_cov_14.983307, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000084 DAORYL010000000
VERSION     DAORYL010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12875
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3816_length_12875_cov_14.983307"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..590)
                     /locus_tag="PLV81_12645"
     CDS             complement(15..590)
                     /locus_tag="PLV81_12645"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOM10989.1"
                     /translation="MKKIIITILAIVLIGIIAVYLFLRTGLPDYSTNINALGLSAPVT
                     IERNSFAVPTITAQNMEDLFFAWGFVNAQDRLFQMEFTRRIAQGRISEFAGADALSKD
                     YFLRGVGFYERAKAYAQKLSPYYKKLYQRYVDGVNHYLDTHGTNVYMKLLGMKKEKWE
                     IADSICVGMMLNWSLANFQSTAFWLHDAHHR"
     gene            complement(749..985)
                     /locus_tag="PLV81_12650"
     CDS             complement(749..985)
                     /locus_tag="PLV81_12650"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOM10990.1"
                     /translation="MEKEKIGITVEGAGVLRKELFKHTVTLDCDARVEDLAKKIKIKK
                     GLQIAFFRNGKRLNRNVKLHDGDTIKVVPLVFGG"
     gene            complement(967..2847)
                     /locus_tag="PLV81_12655"
     CDS             complement(967..2847)
                     /locus_tag="PLV81_12655"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOM10991.1"
                     /translation="MYGNTGKIVYINCSTGKTEVVSLDEDVYKKFIGGSGLAAKLFWD
                     YADFDADPLSEKAVLIFTNGPLAGVPLSGTSRMSAVARSPLTGALAESSCGGYFPPQL
                     RYAGYDGIIISGKCTVPSIIHIAHGSFNIVSSEELWGKTVGEAYVKIKEQYGERSRTL
                     IIGPAGEHGVPYACILSDAHHAFGRCGLGAIMGSKNIKAIVIEPHQTIMKYFEEQKVK
                     SLIKELTPRIKEYIISQVLHEFGSAGNLEGHMYTGDVPIKNWTSNFNEEMAEKLTGST
                     LTEKYLTKTGTCAYCVVACKRIVKVDEGPYSIPEGPGPEYETVAALGCLLGSSDLAAV
                     CKAGRLCNEFGLDSISTGATIAWAMEAFEKGDLTEKDTGGITLRWGDMPTVIDTIIPS
                     IARKEGPLGQLLSKGSRLAAKSINNNLIKYTAQSKGLEAPMHDPRGGGHGHALAYAVS
                     PRGACHVSTAMHFMETGACNYPEIGFEFDLEALTDEKKPETMVLATAIGSIENSACFC
                     QYADRSLTFAEMVGILNAVSGYHYSLQDALDAGWRIYHLKRLINHRFGLRACDDALTE
                     RMLEPARDGEPQGIMINFDSMIQRFYELMNMDNVEGVATVDTLHKYGLEQEAAIVWKK
                     RK"
     gene            complement(2855..3292)
                     /locus_tag="PLV81_12660"
     CDS             complement(2855..3292)
                     /locus_tag="PLV81_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM10992.1"
                     /translation="MGKYYKDTEELYTIYGYFLDNILKDPKIGSKLAKAGIVIKFVYT
                     DPDAEITIDLKNPPKKEGYYGTFYLGKCDIEADVWSSQPADHSHRFWHGLENPIAGVA
                     KGIIKQGGKVTAMLKLLPVIKPTFQQFPIALKEMGREDLIVTK"
     gene            complement(3359..4519)
                     /locus_tag="PLV81_12665"
     CDS             complement(3359..4519)
                     /locus_tag="PLV81_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015946871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOM10993.1"
                     /translation="MDFSYSEEQQMLIKLIHDLGEREKFRDLAREIDKTCQFPFQLIP
                     KYAELGLLGMTLSPEHGGGGQPLLNAIIAIEELAKYSPMIAAPVFESNVGPIKAIDIL
                     GSEFQKKKLIPGVCKGEYSVSVCMTEPEAGSDLTALRTNALEDGDSYVLNGRKAFITG
                     GGHATHYLVYTRFDNIKGYKGIGSLIVEKGMEGFSFGKQEVYMGLHGMPSCDLFFDNV
                     RVPKENVVLKPGEFNRLMATFHIERCGNAAMCLGIAGGALAEAKKYAMEREAFGRPIC
                     EFQAIQFMTVDMAMKLDAARLLVYRAATNAGGGLPSIYEASLAKAYANEMVVDVTNTA
                     MQMFGGYGYSTEFPVERMYRDAKAWGVAGGTIQMLKIGIASMLYGRRFDQRK"
     gene            complement(4554..5918)
                     /locus_tag="PLV81_12670"
     CDS             complement(4554..5918)
                     /locus_tag="PLV81_12670"
                     /inference="COORDINATES: protein motif:HMM:NF015905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HOM10994.1"
                     /translation="MEYTKILSDFCAKLTYNDIPEQVIHKAKLCLLDYIANVYGSLEI
                     EAVKHTIDFIMSITGKNTSTILGITKKTDLLSAAFVNGTTAEAIEAQDGLRFGGNHPV
                     CAVMPAALTVGEKYKKDGKSILTAIIAGYEVANRAAASVHPFHTLSGFLPTGTCGTFG
                     AATTAGKLMQLDEKGMLNAIGNAGYVVPLSMAEHLMGGYTIKIVQGGQAASAGIMAAG
                     LAAHGITGFPKVFEGSELKGGFAQITSKAEPKPERLIDKLGEHFTIMDIYIKPYTGCR
                     HTHGAVQGTLGLIQENNVKPDDVDSILVETYGIAELAVGKHITTNSSFVAAQFSIPYT
                     VSACIFDGDLGPAQITEKRISDSTLIEFSKKVTVKAADDLNAMYPDKTASRVTIKLNN
                     GQIIQKQVDIPKGDPRDPMNVEDIKQKIKRFAKHRKVNADALAEMVMNIEKIKNINEL
                     IKII"
     gene            complement(5956..6756)
                     /locus_tag="PLV81_12675"
     CDS             complement(5956..6756)
                     /locus_tag="PLV81_12675"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOM10995.1"
                     /translation="MSEHPNDYIKPELMACCGAREIQDGDIVIVGTGFPTMSANIARY
                     THAPNAKLMQESGVYDARPLRPALSVGDPCLNPGAAMIGGLVDVMGMFLQGGWIDVGF
                     LAGSQVDKYGNINTTVIGDYNAPKSRLPGSGGANPIGSLAKKVLIISLHDTRHMAEKV
                     DFITTPGYIDGPGSREKWGLPANTGPSVIITNKAVLRFDKTTKVAYLESYHPDTSVDE
                     VVKLTPWKLEVSDNVHETEPPRAEELKALREILDPFRMIKIYEKRGYV"
     gene            complement(6769..7704)
                     /locus_tag="PLV81_12680"
     CDS             complement(6769..7704)
                     /locus_tag="PLV81_12680"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOM10996.1"
                     /translation="MSKIMTTQEAITRFVNDGDFLFIGGYICRPPFAAIHEIIRQKKK
                     DLTVVRSNTADDFDMLIGAGCVKRFICTFISLGLYGLARCFRRSIEKGIPHKIEIEEY
                     TNLSLPLMLMAGALGMPFVPVKDMVGTDLMKIKSFMGDNKYKFIDSPFDGSPVMLVPA
                     LNPDVAIIHVQQADEEGNAQMWGIGGDCKWGANAAKKVIVSCERIVSREVVGKDPSRT
                     IVPGFKVVAVTEEPFGAHPGYTPGFYDVDFSFGYLYQQASNTVEGFQAFLDEWVFGVK
                     NRTEYINHYIQKFGYEQFKKLWAKFDYSYPVSYAY"
     gene            complement(7737..10127)
                     /locus_tag="PLV81_12685"
     CDS             complement(7737..10127)
                     /locus_tag="PLV81_12685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HOM10997.1"
                     /translation="METLRKIVPHYDDFERVNTLRWRIINAPQEVCIERARYLTQSMM
                     HHWEKPALTRMSLALEHILNNISVIIRDDELIVGCRTSKLKGAPLFPENKSRWIEGDC
                     DNFDKRLLQKALITQKEKQELSANILPFWHGKTVEDIFESRLPQDVMEDMDKYIFTMM
                     LEITYGIGHFTMNYSKVLHLGLKGIISHLEQKMHELEVQGEANEKWLLYDAMIRSCKA
                     AIVFAKRYAQKAREMAGKTKRRKRAKELLEIARICEKVPEFPAETFHEAVQSLYFIHL
                     VTQIESGGNSVSIGRIDQILYPFYEKDMKSGRITQDKARELISLLFIKMNEIWNVLEE
                     AYIPGGEGAEGKTTQNVTIGGVDRQGNDVTNELSYIVLDAYADIRTVQPNFSVRISKK
                     TPKEFLIKAVEYARDGVLMHFFNDEIVIETLMKAGHSLEDARDYALVGCVEPNAQGKC
                     FGSTFAVQFSGIKCVEFALSNGVDNIFGYKSGIETGDPSTFHTFDDVWNAYDRQVKHF
                     MNQMARGMEILDKTIAEHVPSPFASVMIDGCIEKGKDLTSGGAVYNSTGVQLIGFANI
                     VDSLYGVKKAVYDQKYCSIAELAQWLSDDWQDVEDKRVYFFRKIPKFGNDNDEVDGLG
                     IKVLEHFCDTVSSHKNFRGGTFWPGVFVVGFHISFGAFTGATADGRHAGDVLGNGLTP
                     TTGNAISGPTAIMNSITKLPLTKIYNGANLNMRFNGNKISTEHLAYLIETYFTKGGMQ
                     VQFNMVDSKILRDAQKNPEKYRDLFVRVSGYSAEFVGLSEIAQEEIISRTEFEYKP"
     gene            complement(10222..11058)
                     /locus_tag="PLV81_12690"
     CDS             complement(10222..11058)
                     /locus_tag="PLV81_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOM10998.1"
                     /translation="MNDVIVITGIADGMGREVAKMLVKEGHCVAGFDKDKELLNSLSK
                     ELESIDGDKVYLEDFDITDRNRIAKFRDKVLEKFKKVDTVLSNVGIGFFGPFEEIDLE
                     KALKCFEINVIGAAAIFKAFIPYMRSLKRGKLIAMSSLVGRIPFPFESIYTATKFALT
                     GLIESIRYEVEPFGIKVALIEPAQVSTKFAAKIHVLPPSSSPYFDRVKRFINRDNELI
                     KTAPDPVYAAKKIYNVVMAKNPRLYNQIDFQSTFFLWLNNFLPVKLRDIILINYMDIK
                     PR"
     gene            complement(11109..12101)
                     /locus_tag="PLV81_12695"
     CDS             complement(11109..12101)
                     /locus_tag="PLV81_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOM10999.1"
                     /translation="MIQYKGITLVTGAAGFMGSHLVEYLAKQGVKVRATSRPRKDTSF
                     FDNLGVEFVPSDLTKPETLPKLFEGDVDRVFHLGAICNFSTPYEVLYHTNVEGVDRIT
                     TLALAHKVKCYVHVGSTSVYGYYKGVPFTEDSPREPQDSYGRSKRDGENIVWGKIKQG
                     LPAIITRPCTVYGPRCNDGAGKVFSRPTRIGAIPGNGRQLLSNVRAEDVAAAVVHLSQ
                     LKEAIGQAYNISDDSHPTVEEALCLAAKVFETTPPRVHMPLPIIKLAARIDGYFSARK
                     GKIPDLEYDAVKYLYDDYVVDNTKLKSTGFRFQYPDFKQSMEQIGEWYKRQKSR"
     gene            complement(12139..12690)
                     /locus_tag="PLV81_12700"
     CDS             complement(12139..12690)
                     /locus_tag="PLV81_12700"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOM11000.1"
                     /translation="MSIKYAILGLLHYTDMHGYRIKDHIEKNFGHMWTINFGQIYTSL
                     KDLEESGLIEVTEIVPSDDGGPHKKCYRITEEGKKDFTRWLHSSPEKQMLLRDPFLLR
                     FAFFGFGDPDRAVEIIDDQIKMYEEQLERRTQNLSRWRKQSVYVRLIADLGVKFNEMY
                     LEWLKQAKEEIAKESEELFVKKV"
BASE COUNT         3744 a         2981 c         2022 g         4128 t
ORIGIN      
        1 taaagtgttc attttcaccg gtgatgagca tcatgtagcc agaaggctgt ggattgaaag
       61 ttggccaggc tccagttaag catcattcca acacaaatgg aatcagctat ctcccacttt
      121 tcttttttca tgcctaaaag tttcatgtac acattggtgc catgagtatc aaggtaatgg
      181 ttaacaccat ccacgtaacg ctggtataat tttttataat agggcgatag tttctgtgca
      241 taagcttttg ccctttcata aaagccaacc cctcgcaaaa agtaatcctt gctcagtgca
      301 tccgctcctg caaactcact tattcgtccc tgagcaattc gccgcgtaaa ctccatctga
      361 aacaacctgt cctgtgcatt gacaaagccc catgcaaaga aaaggtcttc catattctga
      421 gcggtgatag ttggcacagc aaagctgttt ctttctattg ttactggtgc cgaaagccct
      481 agtgcattaa tattggtgga gtaatcaggc aatccagttc gtaagaacag gtaaactgca
      541 ataataccta taagtacaat ggccagtatc gttataataa tctttttcat ggcaaacccc
      601 cgtaataacg ttctctattt aattcactga tagatgagca gtacaaaaag atataatata
      661 ttattaatat gctggaataa taggattgtc aaatactttg tttaaaatac ataggagacg
      721 tgcgccacca ttttgtaaga attattatct aacctccaaa aacaagagga acaactttta
      781 tggtatctcc atcatgaagc tttacattcc tgttcaagcg cttcccattt ctaaaaaaag
      841 ctatctgaag gccttttttt atttttatct tttttgctaa atcttcaacc cgtgcatcgc
      901 aatctaaagt tactgtatgc ttaaacagtt ctttacgcaa aacaccagca ccttcaacag
      961 taattcctat tttctctttt tccatactat tgcggcttcc tgttcaagcc catatttgtg
     1021 taacgtatca actgttgcta ctccttcaac attatccata ttcataagtt cataaaaacg
     1081 ctgaatcatg ctatcaaaat taatcataat tccctgtggt tcaccatcac gtgcaggttc
     1141 cagcattcgt tctgtcaacg catcatcaca agccctcaaa ccaaatctgt gattgatgag
     1201 ccgttttaaa tgatatatac gccatcctgc atcaagcgca tcctgtaatg aataatgata
     1261 ccctgaaaca gcattcagta taccaaccat ttcagcaaat gttagcgaac ggtctgcata
     1321 ctgacaaaag cacgcactgt tttctataga acctatagct gttgccagta ccattgtctc
     1381 tggctttttc tcatctgtta atgcttctaa atcaaattca aaaccaattt caggataatt
     1441 acatgctccc gtttccataa aatgcattgc tgtggataca tgacaggcac ctcgcgggct
     1501 gactgcatat gctaaagcat gcccatgacc tccaccacgt ggatcatgca ttggtgcttc
     1561 caaacctttt gactgtgcgg tgtatttaat caaattatta ttaatacttt tagcagctaa
     1621 tctgctaccc tttgatagta attgtcctaa aggtccttcc tttctggcaa ttgatggaat
     1681 aatggtatct attacggttg gcatatcacc ccatcgcagt gttataccac cagtatcttt
     1741 ttcagttaaa tctccttttt caaatgcctc cattgcccat gctattgttg ctcctgttga
     1801 tatggaatcc aggccaaatt cattacataa tcttcccgcc ttacataccg cagcaagatc
     1861 cgatgaacca agtaaacatc ctaatgctgc cacagtttca tattccggtc ctggaccttc
     1921 aggtatgcta tatggccctt catcaacttt tactatcctt ttacatgcta cgacacaata
     1981 tgcacatgtt ccagtctttg ttaaatattt ttcggttaag gtgctcccag ttaatttttc
     2041 tgccatttct tcattaaaat ttgatgtcca gtttttaata ggaacatccc ctgtatacat
     2101 gtggccctct aaatttccgg cagaaccaaa ctcatgtaaa acctgtgaaa tgatatattc
     2161 cttgatacgt ggagtaagtt ctttaataag actttttact ttttgttctt caaaatattt
     2221 cattatagtt tgatgtggtt caatcacaat agcttttata tttttagaac ccataatggc
     2281 gcccaatcca cacctgccaa atgcatggtg tgcatcactg agtatgcatg catacggaac
     2341 tccatgttca cccgcaggac caattattaa cgttctgctt ctttcaccat attgttcctt
     2401 aatttttaca taagcttctc ctaccgtctt gccccacaac tcttctgaag atacaatatt
     2461 aaaagaaccg tgtgcaatat ggatgataga tggcactgtg cattttcctg atataatgat
     2521 accatcatac cctgcatatc tcaattgtgg agggaaatat ccgccacacg aagattcagc
     2581 cagtgctcct gttaaaggtg aacgtgccac tgcactcatc ctgcttgttc cagacaacgg
     2641 cacacctgca agaggcccat ttgtaaaaat taaaacagct ttttctgata atggatcagc
     2701 atcaaaatca gcataatccc agaaaagctt tgcagccaat ccactcccac caataaattt
     2761 tttgtataca tcttcgtcta atgatactac ttcagttttc cctgttgaac aatttatata
     2821 tactattttg ccggtatttc catacataac ggcctcattt tgtcactata agatcttctc
     2881 tacccatttc cttaagagca ataggaaatt gctgaaatgt tggtttaatt accggcaata
     2941 gtttcagcat tgctgtaacc ttcccaccct gcttaataat tccttttgca acccctgcta
     3001 ttggattttc caacccatgc cagaaacggt gtgagtggtc ggcaggttgc gatgaccata
     3061 catcagcttc aatatcacat ttacccaaat aaaaagtgcc ataatagccc tctttttttg
     3121 gtggattttt taaatctatt gtaatctcag catcaggatc agtataaaca aatttaatga
     3181 caatacctgc ttttgcaagc tttgaaccaa tcttagggtc ttttaaaata ttatccagaa
     3241 aatacccata tattgtatac aactcctctg tatccttgta atactttccc atagtgtcct
     3301 ccaagtataa ttaatttaaa tttgaaatct ccaatctctt accacaaatc accagatatt
     3361 attttctctg atcaaaacga cgcccgtata acatactggc tattccaatc ttcaacatct
     3421 ggattgttcc gccggccaca ccccatgcct ttgcatccct gtacatcctt tcaacaggga
     3481 attctgtact gtatccatat ccgccaaaca tctgcattgc agtattggta acatccacaa
     3541 ccatttcatt ggcatacgct tttgcaagtg atgcttcgta tattgatggc agtccaccac
     3601 ctgcattggt agctgcccgg tacaccagta atctggctgc atcaagcttc attgccatat
     3661 caacagtcat aaactgtatt gcctgaaact cacatattgg cctgccaaac gcttcgcgtt
     3721 ccatagcata tttttttgct tctgccagtg cacccccagc aattcctaag cacatggcag
     3781 cattcccaca tcgttcaata tgaaatgttg ccataagcct gttaaactca cctggtttta
     3841 atacaacatt ctcttttggt actctaacat tatcaaaaaa caaatcacac gaaggcatgc
     3901 catgtaaacc catataaact tcctgcttgc caaaactaaa tccttccatt cctttttcca
     3961 ctatcaaact accaatacct ttatacccct taatattatc aaaacgggta tataccaggt
     4021 aatgtgttgc atgaccacca cccgtaataa aagctttccg gccattgaga acataactat
     4081 cgccatcttc taaagcattt gtcctcaaag cagttaaatc tgatcccgcc tctggctcag
     4141 tcatacatac agacacactg tactcaccct tacaaacacc aggaattaat ttcttctttt
     4201 ggaattcact cccaagaata tcaatagctt tgatagggcc aacatttgat tcaaaaaccg
     4261 gagctgcaat catagggcta tattttgcca gttcttctat tgcaataatg gcatttaaca
     4321 aaggttgccc tcctccacca tgctctggcg aaagtgtcat tcccaaaaga ccaagctcag
     4381 catattttgg aattaattga aaaggaaact ggcatgtctt atcgatttcc cttgctaaat
     4441 ccctgaactt ttctctttcg cctaaatcat gaataagttt aatcagcatc tgttgttctt
     4501 cactatagct aaaatccatg tataataccc ccgggctaaa attttatttg taattaaata
     4561 attttgataa gctcatttat attttttatt ttttcaatat tcataaccat ttcagccaac
     4621 gcatcagcat ttacttttct atgttttgca aatcgtttaa ttttctgctt aatatcttcc
     4681 acattcatag gatcacgcgg gtcacctttg ggaatatcaa cctgtttttg aattatttgt
     4741 ccattgttca gtttgattgt aacacggctt gcagttttat caggatacat ggcattcaga
     4801 tcatctgcgg ctttgactgt tactttcttt gaaaattcaa ttaatgtact atcacttata
     4861 cgtttttctg taatctgagc tggccctaaa tcaccatcaa atatacatgc actcacagta
     4921 taaggaatag agaactgcgc tgcaacaaaa ctactattgg ttgttatatg ctttcccaca
     4981 gccaattcgg caatcccata tgtttcaaca aggatagaat ccacatcatc aggcttaacg
     5041 ttattttcct gtattagtcc taatgttccc tgtactgccc cgtgggtatg ccggcatcct
     5101 gtatatggtt taatatagat atccataata gtaaaatgtt cacccagctt atcaataagc
     5161 ctttcaggct ttggttctgc tttgctggta atttgagcaa agccaccctt aagctcggag
     5221 ccttcaaaaa cttttggaaa acctgtgata ccatgagctg caagacctgc agccataatc
     5281 cctgcactag cggcctgtcc tccctgaaca attttaatgg tatacccacc cataagatgt
     5341 tcagccattg aaagcggcac aacataccct gcattgccaa ttgcattcag catacccttt
     5401 tcatcaagct gcatcagttt gcctgcagtc gttgctgccc caaatgtacc acacgtgcct
     5461 gttggcaaaa aaccggaaag ggtgtggaat ggatgcactg aagctgcagc cctatttgca
     5521 acctcataac ctgcaatgat tgccgtaagg atacttttcc catctttttt atatttttcc
     5581 ccaacagtta gagcagcagg cataactgca caaacggggt gattgcctcc aaacctcaga
     5641 ccatcctgag cttcaatcgc ttcagctgtt gtaccgttta caaatgcagc actcagtaga
     5701 tcagtctttt ttgtaattcc taaaattgtt gatgtatttt ttccggttat ggacataata
     5761 aaatcaatgg tgtgtttaac agcttctatc tctaaagaac catatacatt agcaatatag
     5821 tcaagaagac atagttttgc tttatggatc acctgctcgg gaatgtcatt atatgtaagc
     5881 tttgcgcaga agtccgaaag tatttttgta tattccatac tttcctcctt ttatacatct
     5941 aaatattatt attgcctata cgtatccacg cttttcataa attttgatca tcctgaatgg
     6001 atccagtatt tcacggagtg ctttcaattc ttcagcacga ggtggttctg tttcatgaac
     6061 gttatcactg acttccagtt tccagggagt aagcttgacc acctcatcca cacttgtatc
     6121 agggtgatag ctttccaaat atgccacttt agttgtttta tcaaaacgca gcactgcttt
     6181 attggtaata ataacagaag gaccagtatt ggcaggcaag ccccactttt cacgggaacc
     6241 gggaccatct atatagcctg gtgttgtaat aaaatcaact ttttcagcca tatgacgggt
     6301 atcatggagt gatattataa gcactttttt ggccagcgag cctattggat ttgccccacc
     6361 agatcccggg agtctgcttt ttggcgcatt gtaatcacca atgactgttg tattgatatt
     6421 gccatattta tccacctggc ttccagctag aaatccaaca tctatccagc caccctgaag
     6481 gaacataccc atcacatcaa caagcccccc aatcattgca gcaccgggat taagacaagg
     6541 atcacccacc gataaagcag gccttagcgg ccgagcatcg tatacaccgg attcctgcat
     6601 gagctttgca ttgggagcat gcgtatatcg cgcaatattt gctgacatcg ttgggaatcc
     6661 tgttcccact atgacaatat ctccatcctg tatttcacgc gcaccacagc aggccatcaa
     6721 ttctggttta atataatcat tgggatgttc tgacatggca aacctccttt aatatgcgta
     6781 tgatacaggg taactgtaat caaatttggc ccataatttt ttaaattgct catagccaaa
     6841 tttctgtata tagtgattga tatattccgt tctgttttta acaccaaata cccattcatc
     6901 aaggaaagcc tgaaagcctt ctacagtatt tgatgcttgc tgataaagat atccaaatga
     6961 aaaatccaca tcatagaatc ccggagtata acccggatgc gccccaaagg gttcttcagt
     7021 tactgcaaca actttaaacc cgggaacaat ggttcttgaa gggtctttcc ctacaacctc
     7081 acggcttaca atcctttcac aacttactat caccttcttt gccgcattag ctccccattt
     7141 acaatcacca ccaatacccc acatctgggc attgccttct tcatctgcct gttgaacatg
     7201 aataattgca acatctggat tgagtgcagg caccagcatt accgggcttc catcaaatgg
     7261 tgaatcaata aatttatatt tgttatcacc cataaatgat tttatcttca ttaaatcggt
     7321 acccaccatg tcctttaccg gtacaaacgg cataccaagc gcacctgcca taagcatcag
     7381 tggcaatgat aaatttgtat attcttcaat ttcaattttg tgaggaatgc ccttttctat
     7441 tgatcgtctg aaacaccgtg caaggccata caagcccagt gaaatgaatg tacaaataaa
     7501 ccgttttaca caaccagcac ctatgagcat atcaaaatca tcagcagtat tactgcggac
     7561 aacagttaga tcttttttct tctggcgtat aatttcatgt atagcagcaa atggcggcct
     7621 gcagatgtaa ccaccaataa aaagaaaatc accgtcatta acaaatctgg tgattgcttc
     7681 ctgtgtggtc attattttgc tcatactttc ctccaataaa atcaatagat gtatttttat
     7741 ggtttatact caaattcagt gcggctaatt atttcttctt gagctatctc tgatagtcct
     7801 acaaattcag ccgaataccc gctgacgcga acaaataaat ccctgtattt ttccggattc
     7861 ttctgtgcat cacgtaaaat ctttgaatca accatattaa attggacctg cattccacct
     7921 tttgtaaaat atgtttcaat gagataggct aaatgttctg ttgatatttt gttaccattg
     7981 aacctcatat tgagattggc accattatat atttttgtta acggaagctt tgttatggag
     8041 ttcatgatgg cagttggacc tgaaattgca tttcccgttg tgggagttaa tccattacct
     8101 aaaacatctc ctgcatgacg accgtcagcc gtagctccag taaatgcacc aaagcttata
     8161 tgaaaaccta ctacaaacac accaggccag aatgtacccc ctctgaaatt tttatggctg
     8221 cttacagtat cacaaaaatg ttccaggacc ttaattccca atccatcaac ttcatcattg
     8281 tcatttccaa attttgggat tttcctaaaa aaataaactc ttttatcttc aacatcctgc
     8341 caatcatcag aaagccattg tgccagttca gctatactgc agtacttctg gtcatacacg
     8401 gccttcttta ctccatacag actatcaaca atattggcaa accctataag ctgcactcct
     8461 gttgaattgt agacggcacc acccgatgtt aaatcctttc ccttttctat acaaccatca
     8521 atcatcactg atgcaaatgg tgaaggtaca tgttcagcta tagttttatc aagaatctcc
     8581 atgccacgcg ccatctgatt catgaaatgt ttcacctgac ggtcataagc attccataca
     8641 tcgtcaaacg tatgaaatgt gctggggtcg cctgtttcaa ttccactttt atatccaaaa
     8701 atattatcaa caccattgga tagagcaaat tccacacatt taataccgct gaactgaaca
     8761 gcaaaagttg agccaaaaca tttaccctga gcatttggtt caacacagcc aacaagcgca
     8821 tagtcacgag catcttcaag agaatgcccg gctttcatca aagtctcaat aactatctca
     8881 tcattaaaaa agtgcatcaa cacaccatca cgggcatatt caacagcttt aattaaaaat
     8941 tcttttggtg tcttctttga aatcctaaca ctgaaattag gctgtactgt ccgtatatca
     9001 gcataagcat caagaactat gtacgatagt tcattggtaa catcattccc ctgcctatca
     9061 actccaccta tggttacatt ctgagtggtt ttcccttctg caccttcccc accggggatg
     9121 tatgcctcct ccagaacatt ccagatttca ttcatcttga taaacaacag tgaaataagc
     9181 tcacgcgctt tatcctgagt gatacgtcct gatttcatat ccttttcata gaatggatat
     9241 aaaatctggt caatccttcc aattgaaacc gaattgcctc ccgattcaat ctgagtaacc
     9301 agatggataa aatacagcga ttggacagct tcatgaaatg tttcagcagg gaattcaggc
     9361 accttttcac aaatacgtgc tatttcaagc aactcctttg cacgcttacg cctttttgtt
     9421 ttaccagcca tttctcgtgc tttctgagca tatcgtttgg caaatactat cgccgccttg
     9481 catgaacgta tcattgcatc atacagcaac cacttttcat tagcttcgcc ctgtacctca
     9541 agctcatgca ttttttgttc cagatgggaa ataatccctt tcaaaccaag atgtaaaacc
     9601 ttagaataat tcatggtaaa gtgaccaatg ccgtacgtta tctcaagcat catggtaaag
     9661 atatatttat ccatatcttc catgacgtct tggggtaatc ggctttcaaa aatatcttca
     9721 actgttttgc catgccagaa aggaagtata ttagccgata gttcctgctt ttctttctgg
     9781 gtaatcaatg ctttctgtaa taggcgctta tcaaaattat cacaatcgcc ttcaatccag
     9841 cgcgacttgt tttcaggaaa taacggagca ccttttaact ttgaagttct gcatccaacg
     9901 atcagttcat catcacgaat gattacacta atattattca atatatgctc cagtgccagg
     9961 ctcattcttg ttaaggcagg cttttcccag tgatgcatca tggattgggt caaatatcgt
    10021 gcccgttcaa tacaaacttc ctgtggagca ttaattattc tccatcgcaa tgtattcacc
    10081 ctttcaaaat catcataatg aggtactatt ttccgtaatg tttccatatt attacctccc
    10141 ttacacttat acgtgcatca caggatacac tgaaaaatct gtattcaata tacccaaact
    10201 ccatatgaaa aactactatg gctatcttgg tttgatatcc atgtagttta tcaggataat
    10261 gtctcttaac tttacaggca aaaaattatt cagccacagg aaaaatgtgc tttgaaaatc
    10321 tatctgattg taaagcctgg gattttttgc catcactacg ttataaattt tctttgcagc
    10381 atatacagga tcaggtgctg ttttaattaa ttcattatcc ctgttaatga aacgtttaac
    10441 ccggtcaaaa tatggtgaac tagatggcgg taacacatgt atttttgctg caaatttagt
    10501 tgacacctgt gcaggctcaa taagtgccac tttaatgcca aaaggctcaa cttcataccg
    10561 tatagattca ataagccctg ttaaggcaaa ttttgttgct gtatatattg attcaaacgg
    10621 gaaaggtatt cttccaacaa gagaagacat ggcaattaat ttccctcttt tcagacttcg
    10681 catatagggg ataaatgctt taaatattgc tgcagcacca atgacattga tttcaaaaca
    10741 tttaagggct ttttctaaat caatttcttc aaaagggcca aaaaaaccaa tacccacatt
    10801 tgaaagaact gtatccactt ttttaaattt ttctaaaacc ttatcacgaa attttgctat
    10861 acgatttcta tcagtaatat caaaatcctc taagtagacc ttatctccat ctatggattc
    10921 cagctctttg ctaagtgaat tgagtaattc tttatcttta tcaaaaccag ccacacagtg
    10981 gccttctttg acaagcattt tggccacttc tcttcccata ccatcagcaa taccggttat
    11041 tacaataaca tcattcatat acgttcctcc aatttaataa ttattattta tattattctt
    11101 tttaaccttt atctgctctt ttgccgttta taccattcac ctatttgttc cattgattgt
    11161 ttaaaatcgg gatattgaaa cctgaacccg gtagatttta atttcgtatt atccacaaca
    11221 taatcatcat aaagatattt aacagcatca tactccagat cgggtatttt gccttttctg
    11281 gctgaaaaat aaccgtcaat tcttgcagct aatttgataa tgggcaatgg catgtgaact
    11341 cgtggcggtg tggtttcaaa tacctttgct gccaaacata atgcttcttc aactgttgga
    11401 tgtgaatcgt ctgaaatgtt atatgcttgc cctatggctt cttttagctg tgaaagatgc
    11461 actacagcag ctgctacatc ttctgcacgt acatttgaca ataactgcct gccatttccg
    11521 ggaattgcac caatcctggt tggtcgtgaa aaaaccttgc cagcaccatc attacagcga
    11581 ggaccatata ctgtacatgg ccttgtaatg attgcaggaa gtccctgttt tatttttccc
    11641 cagactatat tttctccatc tcgtttactt ctgccatacg aatcctgagg ttcacgtggg
    11701 ctgtcttcag taaagggaac acctttataa tatccatata cacttgttga acctacatga
    11761 acataacatt taactttatg ggcaagtgcc agtgtggtta tcctgtcaac accttctaca
    11821 ttggtatgat aaagaacttc atagggtgtg gaaaaattac atatagcacc aagatgaaaa
    11881 accctgtcta catcaccttc aaaaagtttt ggtagtgttt cgggttttgt taaatcggaa
    11941 gggacaaact caacacctaa attatcaaaa aatgaagtat ccttacgagg tcgtgatgtt
    12001 gcacgcacct taacaccctg tttagccaga tattcaacaa ggtggcttcc cataaatcct
    12061 gcagcaccag taaccagtgt aatcccttta tactgtatca tactttcctc caggcaatac
    12121 accaactgta ttgatatgtt atactttctt tacaaaaagc tcctctgatt cttttgctat
    12181 ttcttcttta gcctgtttca gccattctaa atacatttca ttaaacttaa ctcccagatc
    12241 agctatcagc ctgacatata cactctgctt tcgccagcgt gaaagatttt gtgttcttcg
    12301 ttcaagctgt tcctcataca tttttatctg atcatcaatg atttccactg cccgatcggg
    12361 atctccaaat ccaaaaaaag caaaacgcaa caaaaaaggg tctcgcagca gcatttgttt
    12421 ttcaggcgat gaatgaagcc accttgtaaa atcttttttc ccttcttctg ttatgcgata
    12481 gcacttttta tgaggcccac cgtcatctga cggaacaatc tcagtgactt caattaatcc
    12541 gctttcctca agatctttta aactggtata tatctgccca aaattaattg tccacatgtg
    12601 gccaaaattc ttttctatat ggtcttttat gcgatagcca tgcatatcag tataatgaag
    12661 cagacccaga atagcatatt ttatagacat gtagatatcc tttacaaata ttaaatttta
    12721 actatataca ttatatatat atactttata tatagtgtca agtatttttt ttggattttt
    12781 tttagaaaaa ttttatccat aaaaaaaagg aatgcacttc ctgcattcct tttcatactc
    12841 ttcaaagttt taaaattaaa ttcttcttta ccgca
//
LOCUS       DAORYL010000085        12809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_3839_length_12809_cov_13.157676, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000085 DAORYL010000000
VERSION     DAORYL010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12809
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3839_length_12809_cov_13.157676"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..1041
                     /locus_tag="PLV81_12705"
     CDS             229..1041
                     /locus_tag="PLV81_12705"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOM11001.1"
                     /translation="MSEAHITLEFKDKIAIVTLNRPARLNAFNDEMFDKLERITAELL
                     NALPRSVIITGSGERSFSAGFDVQPDNPMVKRIVDAVSTKDKSPAKQAIAHIRRVVDA
                     FVSMPVPIIAAINGDAYGGGAELAVRCDLRVMSRDAVFCFSEAKLGLMTDWGGGATLV
                     QLVGASKAADLLLTARKVFADEALGIGLVNRVVDKDAVMPEAIAIAGQIAENGPNAVR
                     HMLALVRNSRNISLDASLQEEAERAVSLIVSGECFHGVSAFLERRKPEFPDV"
     gene            1163..1235
                     /locus_tag="PLV81_12710"
     tRNA            1163..1235
                     /locus_tag="PLV81_12710"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1196..1198,aa:Cys,seq:gca)
     gene            1240..1325
                     /locus_tag="PLV81_12715"
     tRNA            1240..1325
                     /locus_tag="PLV81_12715"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1274..1276,aa:Leu,seq:taa)
     gene            complement(1346..1687)
                     /locus_tag="PLV81_12720"
     CDS             complement(1346..1687)
                     /locus_tag="PLV81_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11002.1"
                     /translation="MILYQRITLTAGILLILSGCTHLTQLVVYQHHGHVIAASLFGLA
                     YLVIGILLVIEKQWVLWIATLLPLIGGILGVIRFIYMHTNPFSFFHVLLDMVIVPFCL
                     YVLVRKKDMGR"
     gene            1945..2706
                     /locus_tag="PLV81_12725"
     CDS             1945..2706
                     /locus_tag="PLV81_12725"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOM11003.1"
                     /translation="MNKRRYVVISIVALLVVISGGVICSRNEKTLKPKIGPVVEAVYA
                     LGIVTPYKEFSLRVGMSTKIERLFVWEGDAVSAGSPLVQLDGGIILRSPFSGTIAKLN
                     RKENEIVTAQESIMTIVDPKKMYVRLSLDQESIINVRKKQKAQVSFENLRGTIMTGYV
                     SRIYPSDGEFVVTVDVDSFPEGVLPYMTCDVAIVVREKDRALFIPQNAVINNMVYVIR
                     NRKKLMVKVTTRNFKDGWLEVLEGDVKPEDSIVVR"
     gene            2715..3941
                     /locus_tag="PLV81_12730"
     CDS             2715..3941
                     /locus_tag="PLV81_12730"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOM11004.1"
                     /translation="MFELAIKYIFSRKLQSLLMLIGVAFGCGGYIVFTSIQTGFQNFL
                     KERLIENNGHITIRSREDYITTDSLAGVFFQGKNIYWITEPSGKRSHDYLQSAWYWQK
                     RLEKLPYVVANTRRIEATTTCSRGSFSRSIALRGIDPVNEPKVTNIAEDIIEGKLQML
                     SSGNNMVIVGVELLKFLGAKVNDTINIINPNGHVYPVKIVGIYSSGDRRNDESLVLGS
                     ITTVQNILESPGKITKIVVKTVDANKAALFAQELSHYSYDKVESWDQANINFLSMTKQ
                     QTLIRQITMFTFILVVSFGIYNVLNMLVYQKQREIAILRSIGYTSADTVILFLLQGAL
                     ISIAGGVIGLIAGYAACSYIQTIKLPGRPMMVAWDLSIYIWGFMLVIISALFASFLPA
                     KNAGRLSPIEIIRRTE"
     gene            3941..4618
                     /locus_tag="PLV81_12735"
     CDS             3941..4618
                     /locus_tag="PLV81_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOM11005.1"
                     /translation="MMAIEAQKVVKSFGDFAALKSITLNIPGGEFVAITGKSGSGKST
                     LFYSLSGLDTINSGHVRVLGKNIHAMPQDELHDFRNTKMGFVFQFHYLVSELTGLENI
                     LLPAKKKNLHKEKREYALYLLDYFAIAHCKDKLPSQMSGGERQRVAVARSLIMNPEII
                     FADEPTGNLDSINSEIVMNIFREINLKNKTTVILITHDDEYAHLADREIRLKDGEIES
                     DVHYKKR"
     gene            4633..4767
                     /locus_tag="PLV81_12740"
     CDS             4633..4767
                     /locus_tag="PLV81_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11006.1"
                     /translation="MSEMRCADGSYDGYNDDTGKYSLTVGPLYYEFTEDEDEDDEESE
                     "
     gene            4751..6238
                     /locus_tag="PLV81_12745"
     CDS             4751..6238
                     /locus_tag="PLV81_12745"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOM11007.1"
                     /translation="MKKVNNVFRINKIALQTIISVLYSILAILYCIPLSPYPIIAQEN
                     ATIISYEQYISVITQKLPQIKNNVLQVEKAYNQVEKAKSVDDTNLIAKGQYARQKMYT
                     TGKSLYEPDYKQSYYGYTGIEQTLASTGTRVSLGFEYNASSITGNLTIPPQAYSVDEY
                     RPSITATITQPLLKNAFGIIDRYVKNSAAMKLEIQKLQQLQDDQSVLNYYKKLYFNWV
                     LYKQALDIWQESITNARSLVDSVKRKARAGLAENDDVQRAVSSLLNYEAQYKQYEIAY
                     KSLCDELSLYFDTSITPHINDMNSMFDNVVSAEYTEVPFEKTRSWLIVKKNMDVMQYT
                     INVADNSTLPQMNLILSASRKNYSDRQREALSKLPDTDYSVGVEFSYPLGNHDADAQL
                     KDYELSLKELEHNLLITQNSYYKSLRTIMQYLNGYKELIATHNKNLQALISQRQTERK
                     KYEQARLDLQYLINTENSIAMEKLTLLQLKVGLLGYYIDYKDLTE"
     gene            6312..7193
                     /locus_tag="PLV81_12750"
     CDS             6312..7193
                     /locus_tag="PLV81_12750"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOM11008.1"
                     /translation="MYTITPTYFYSNNYKLDARLYLPDTGKKTPVIVMAHGFGARMDW
                     GLHPFADIFAQMGCAVLMFDYRGFGNSEGNIRRLVYHVHHIEDYHMAIQYAMTIPNID
                     SSKVLLWGTSFSGGHVLTVASQDSSIAGVIAQVPFVDGVATAFHLPVKNILSGLWYGS
                     LDVVMSLITSKPVTIPLVATPDTFAAMNTPECYDGYMKLVPPEARDENWCPARVCLTL
                     PLYRPIAVVDKISCPVCIIGAQHDSLIPIKAVEKTAKRIKHVDFHILPCGHFDPYDGE
                     MFDKNIQIQKQFLQKVL"
     gene            7577..7747
                     /locus_tag="PLV81_12755"
     CDS             7577..7747
                     /locus_tag="PLV81_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11009.1"
                     /translation="MLGLGDVWVFMAYLLCIASAILCAVYGFIKWNDDEEPYTDEAKR
                     WVQEEAEIEKTL"
     gene            7764..9533
                     /locus_tag="PLV81_12760"
     CDS             7764..9533
                     /locus_tag="PLV81_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012193735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOM11010.1"
                     /translation="MTIFWVFVYLVIVAFLGYLGYRHTKGASDYLVAGRKVHPFIMAL
                     SYGATFISTSAIVGFGGAAGVFGMGLLYLTFLNIFVGIFIAFVFFGKRTRRMGYHLDA
                     HTFPEFLGRRFQSPFVQKFAGIIIFLFMPLYAAVVLIGAAKYIEQQFAIDYATALLFF
                     TLIVAVYVIMGGLKGVMYTDAFQGSIMAIGMLTLIIFTYYDLGGIIKSHADLTVLTPP
                     PPLAAKGHMGWTSMPALGSDFWWTLVSTIIMGVGIGVLAQPQLVVRFMTVKSNRELNR
                     AVAIGGIFILLMTGVAFTSGALSNVWFTKNEKGVKYCEISGAILEVCPGKKVVKIDEK
                     NIEKFKAQFKDIKPGDEIDLHKGIPHKETTKGSLAIVAAGGVVENIIPQFVKQAMPHW
                     FAVLFMLTLLAAAMSTLSSQFHAMGTAIGRDFYMQILKDKKAAEERSVFINKMGIGVT
                     ILVSLTLAYFLPVIYSKGEAIIARGTSIFFGLCAGTFLPAYISALYLKRITKAGVVAG
                     MLSGFTVAFFWLVFVLEKEAMAIGLCQALFGKPTLLSSPWTMVDPMFISFPTSLVVTL
                     VVSLFTKKLPEEHVTHCFQGLEK"
     gene            complement(9776..10852)
                     /locus_tag="PLV81_12765"
     CDS             complement(9776..10852)
                     /locus_tag="PLV81_12765"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOM11011.1"
                     /translation="MEYTTIALVFIVILLVLIAFKLFFAKGDTSSELAVQLIDLKNQI
                     VELKTKQLEAYNTALENQQRNLSQLISTVNEILTSTQKNITQQLNNTDKVVSDIHTKL
                     GSLEQTAHQIREIGNDIASLQDILAAPKLRGNLGEYLLEELLKQFLPAKNYHMQYTFK
                     NGTKVDAIIQLGGQLIPVDSKFPLESFQRIIKATSDNEKNQYKKEFIRAVKQRIDEIS
                     QKYINPDEGTYDFALMYIPAENVFYEIIVNDTVSDKAYEIFNYALASKVIPVSPNSFY
                     AYLMAIAYGLKGLQIEQKAKDILNGLATVQNQLKNFEDEYSITGKHLRNALTSYERSQ
                     RQVEKINDKIASFTGKHIDVLPES"
     gene            complement(10876..11931)
                     /locus_tag="PLV81_12770"
     CDS             complement(10876..11931)
                     /locus_tag="PLV81_12770"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HOM11012.1"
                     /translation="MLLFSIGFILTILMQSSSAAVVLALVALSSGTISLFQGQVLVIG
                     MNAGKSVPILFAAIGATPDTRRIIVADVLFNTVTALVLFVLLPQLSPLLSSLCNAFGA
                     IVPEIELSVFHTLFNVIGVLLFFPFITPFVRFLKTLFTDTRFYLTQHLDDAIATTPAI
                     AVETARRTVVDIALYIIQILKDMLQKNGTDIAHRLKTAQIALEETKHFMSKVKTPPQQ
                     KQLYDEHISVLHAIDHLNSIIEACQEAHIVSLLKNSTNFSDMKWNFNTELVESEKALK
                     AESSIDSVEKIQELSQYLANSRKLERVEVLKKTATGQIDSHTALDYIEAIRFIDRLGY
                     HIWRVVRHCIVPGDSNY"
     gene            complement(11943..12461)
                     /locus_tag="PLV81_12775"
     CDS             complement(11943..12461)
                     /locus_tag="PLV81_12775"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HOM11013.1"
                     /translation="MNISDIITAIGGIGILLLGIVLMSEAIQKLAGPRFRKILANLTG
                     GKFKALFTGMLVTTIIQASSATAIATIGFVSAGLITIPQALAVIFGANVGTTATAWLV
                     TMFGIQYSIGQFSLLFVACGVILKILYTDHRSDIGSLLAGFGLLFFGITIMRQGLAQL
                     PQQLILLPSQDM"
     gene            complement(12492..12785)
                     /locus_tag="PLV81_12780"
     CDS             complement(12492..12785)
                     /locus_tag="PLV81_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOM11014.1"
                     /translation="MTVLDGIERKTLVFGTHTLMTEFRLQKGKKLPIHKHPHEQTGYL
                     VKGHIILIMDGQTYDMKPGGSWSIPGNIEHGAEVLEDSIAIEVFSPVREDYIP"
BASE COUNT         3817 a         2257 c         2761 g         3974 t
ORIGIN      
        1 ttaaaatgaa cataaattgt aatatgttaa tactgcattt accaccagtt gttcaggtta
       61 tcttgtttta atatttttat ataacgatag cttatgtttt ttaaatgttt tttattgcca
      121 ttttaatggt tatttgaaaa tcttattatt agtgatggac taattgagaa tacgatagtt
      181 attctattat gcattttgat gtaaaaatct atatgaaagg ggggatacat gtctgaggca
      241 catatcacac ttgaatttaa ggataaaatt gcgatagtta ccctcaatcg gcccgcgcgt
      301 ttgaatgctt ttaatgatga gatgttcgac aaactggaac gtattactgc agaattgctt
      361 aatgcattgc cccgttcagt cattatcact ggaagcggcg agcgctcttt cagtgctggc
      421 tttgatgtgc agcccgacaa ccccatggtg aaacgcatag ttgatgcagt cagcacaaag
      481 gataaatcgc ctgccaagca ggcgattgcg cacatacggc gcgtggttga cgcgtttgta
      541 tctatgccag ttcccataat cgcggcgata aatggcgatg cttatggagg gggtgcggag
      601 cttgcagttc gctgcgatct ccgtgtaatg tcacgagatg cagtgttctg tttttcggaa
      661 gcgaaactag gcctcatgac cgactgggga ggtggggcga cgcttgtgca attggtggga
      721 gcatcaaaag cggctgattt gctcctcacc gcgcggaaag ttttcgccga tgaagcgctt
      781 ggaatcggcc ttgtaaatcg ggtggtcgat aaagatgccg taatgcctga agcgattgcc
      841 attgccggac agattgcgga aaacggaccg aacgctgtgc gccacatgct tgcgctggtg
      901 agaaacagca gaaacatttc cctggatgcc tcgctccagg aggaagcgga gcgcgcggtt
      961 tcgcttattg tgtcagggga gtgctttcac ggcgtttctg catttctgga aagaagaaaa
     1021 cctgagtttc ccgatgtgtg atgtggtgtg ggtgaacacg aagaaaaagt caaactcttt
     1081 agaggcaaaa aacaaaaatt ttctgttgac aataaaaatg tgaatttgta gaggtgaatg
     1141 tgtatattat gatactatac acggcgccgt acccaagtgg ctaagggaga ggtctgcaaa
     1201 acctttattc agcggttcga atccgctcgg cgcctatacg cccaagtggt ggaacaggta
     1261 gacacaaggg acttaaaatc ccttggactt tcaagtccgt gccggttcaa gtccggcctt
     1321 gggcacattg tttatcttac agtaactatc gccccatatc ttttttccgt accagcacat
     1381 ataaacaaaa aggtacaatt accatatcaa gtagtacatg aaaaaaactg aaagggtttg
     1441 tgtgcatata tataaatcgt attacaccca gtatgcctcc aatgagtggc agtaatgttg
     1501 caatccataa aacccattgt ttttcaatga caagcagtat gcctatgact aaatacgcca
     1561 gaccaaataa tgaagcagca ataacatgcc catgatgctg gtaaaccacc agctgggtta
     1621 gatgtgtaca tcctgaaagt attaatagta ttcctgcagt aagggtaatt cgctgataca
     1681 gtatcatggt aaacctcact tattgtatga gattatcttt ttataaattg tatttgcaca
     1741 gtgatgaatt ttcaatcatt attgtaaaat tattcaatca atggtattat accctgcagt
     1801 atatttttta aggatttttc aggaaatgat actatgcaaa gatgtaattg tatgtatgaa
     1861 taatgaaata aaatggaacg ttatggtatt gtgtagggtc taaaaataaa tataaatttt
     1921 tttatataac aatcagaggc caggatgaat aaacgcaggt atgtagtgat tagtatagtg
     1981 gcacttcttg ttgtgataag tggcggtgtt atttgctctc gtaatgaaaa aaccctgaag
     2041 ccaaaaattg ggccagttgt agaggctgtg tatgcattgg ggattgtaac accgtataag
     2101 gagttcagcc tgcgtgtggg gatgagcaca aaaattgaac gcctctttgt atgggagggt
     2161 gatgcggtaa gtgcaggcag cccgctggtt cagcttgatg gcggtattat actgcggtca
     2221 cctttttcag gaactatcgc caaattaaac cgtaaagaaa atgagatagt taccgcacag
     2281 gaatccatta tgactatcgt tgaccccaaa aagatgtatg ttcgtctttc gcttgatcag
     2341 gaatccatca tcaatgtcag gaagaaacaa aaagctcagg tgagttttga aaatttgcgt
     2401 ggtacgataa tgaccggtta tgtaagccgt atttaccctt cggatggcga atttgtagta
     2461 actgttgatg tggatagttt ccctgaagga gtgcttccct atatgacctg cgatgtggca
     2521 atagtagtga gggaaaagga cagggcgctc tttatacctc agaatgcagt aattaataat
     2581 atggtttatg tgattagaaa caggaaaaaa cttatggtaa aagtaacgac gcggaatttt
     2641 aaagatggct ggcttgaggt gcttgaaggt gatgtaaaac ctgaagacag cattgttgta
     2701 cggtaaataa tgccatgttt gaattagcca ttaaatatat tttttccaga aagctgcaat
     2761 cattactcat gcttattggt gtggcgtttg ggtgtggcgg gtatattgta tttacatcaa
     2821 tacagactgg ctttcagaat tttttgaaag agcgacttat agaaaataac gggcacataa
     2881 caataagaag cagagaggat tacataacaa ccgattcgct tgctggtgtt ttttttcaag
     2941 gcaagaacat ttactggatt actgaacctt cgggaaagcg ttcacatgat tatctgcaga
     3001 gcgcatggta ctggcaaaag cgcctggaaa agctacccta cgttgtggca aacacacgtc
     3061 gaatagaagc aacaacaacc tgtagccgcg gatcgttttc tcgttcaatt gctttacggg
     3121 ggattgatcc tgttaatgaa ccaaaagtaa caaatattgc ggaggatata atagaaggga
     3181 aattgcagat gctttccagt ggcaacaaca tggtcattgt gggagtggaa ttgctgaagt
     3241 ttttaggtgc aaaagttaat gatactatca atattattaa tcctaatggg catgtgtatc
     3301 ctgtaaaaat agttggcatt tattcttcag gtgacaggcg aaatgatgag tcgctggtgt
     3361 tgggttccat taccacggta cagaatattt tagaaagccc tggtaaaatc acaaagatag
     3421 tggttaaaac tgtagacgca aacaaagcag cattatttgc tcaggaacta tcacactaca
     3481 gttatgacaa agtggaaagc tgggatcagg ccaatataaa ttttttaagc atgacaaaac
     3541 agcaaacact catacgccag atcaccatgt ttacttttat tctggtggtc tcatttggca
     3601 tatataatgt gctcaatatg cttgtctatc agaaacagcg agaaatagct atcctgcgtt
     3661 ctattggata taccagtgct gatacggtga ttttattcct cttgcaggga gcacttatca
     3721 gcatagcagg gggtgttata gggcttattg caggttacgc tgcgtgtagc tatatccaga
     3781 ctattaagct gccgggaagg cccatgatgg ttgcctggga tttatcaata tatatatggg
     3841 gttttatgct ggtgatcatt tctgcactgt ttgcaagctt tttaccggca aagaatgccg
     3901 gtagactctc gcccattgaa attatacgga gaacagagtg atgatggcaa tagaagcaca
     3961 aaaagtggtg aagtcatttg gtgattttgc cgctttaaaa tcgatcactt tgaatatacc
     4021 cggcggtgaa tttgttgcaa ttactggcaa atcagggtca gggaaatcca cgctctttta
     4081 ctctttaagc ggacttgata ctataaatag tggtcatgtg cgtgttcttg ggaaaaacat
     4141 acatgcaatg ccccaggatg agctgcatga ttttagaaat actaaaatgg gttttgtgtt
     4201 tcagtttcat tatttagtct cagagctgac agggcttgaa aatatccttt tgcctgctaa
     4261 aaagaaaaac ctccataaag aaaaacgtga gtatgctctg tatctattag attattttgc
     4321 gatagctcat tgtaaggata aattgccgtc acagatgtca ggtggcgagc gccagcgtgt
     4381 tgctgttgcc cgatcactta tcatgaaccc tgaaataatc tttgctgatg aacctaccgg
     4441 taatttagat tccatcaaca gtgaaatagt aatgaatatt ttccgtgaga taaatttgaa
     4501 gaataaaacc acggtgattc tgattaccca tgatgatgag tatgcacatt tagccgacag
     4561 ggaaattcgt ttaaaggatg gagaaattga atctgatgtg cattataaaa aaaggtagag
     4621 gcgtatggtg atttgtcaga aatgcgttgc gctgatggtt catacgacgg atataatgat
     4681 gatactggga aatattcctt aacagttgga ccactgtatt atgaatttac tgaggacgag
     4741 gatgaagatg atgaagaaag tgaataatgt tttcaggata aacaaaattg ctctacagac
     4801 cattattagc gttttgtata gcattcttgc catactatat tgtattcctt tatcaccata
     4861 tcctatcatt gcacaggaga atgctactat catttcatac gagcaatata tatcagtaat
     4921 tactcaaaag ttgcctcaga ttaaaaataa cgttcttcag gtggaaaaag cctacaatca
     4981 ggtggagaaa gctaaaagtg tggatgacac aaacctgatt gcaaaggggc agtatgcacg
     5041 gcagaagatg tacacaactg gtaaaagcct ttatgaacct gattacaaac aatcgtatta
     5101 cggatatacg ggcattgagc aaacattagc ttcaaccgga acccgtgtgt cattaggttt
     5161 tgagtataat gcttcatcta tcacaggtaa tctgacaatt cctccacagg cttattccgt
     5221 tgatgaatac agaccttcta taacggcaac tatcacacaa cctcttttaa aaaatgcttt
     5281 tggtataatt gaccggtatg tgaaaaatag tgcagcaatg aaattagaaa tacagaaact
     5341 tcaacagtta caggatgatc aatcagtatt gaattactat aaaaagcttt atttcaactg
     5401 ggttttgtat aaacaggcgc ttgatatatg gcaggaaagt attacaaatg caaggtcact
     5461 ggtggattca gtaaagcgca aagccagggc cgggcttgct gaaaatgatg atgttcagcg
     5521 agcagtatca tcacttttga actatgaggc acagtataaa caatatgaaa ttgcctataa
     5581 atctctttgt gatgaattat cgctttactt tgatacgtct attaccccgc acattaatga
     5641 tatgaatagt atgtttgata atgtagtttc agcagaatat accgaggtgc cgtttgaaaa
     5701 aacgcgttca tggcttattg taaaaaagaa catggatgtt atgcagtata caataaacgt
     5761 tgctgataac agcactcttc cacaaatgaa cctgatttta tcggcatcac gaaaaaatta
     5821 tagtgaccgg cagcgcgaag ctttaagcaa attacccgat acagactatt cggttggggt
     5881 tgaatttagt tatccattgg gtaaccatga tgcagatgcg cagcttaaag attatgagtt
     5941 atcactgaaa gaattggaac ataatctttt aataacacag aatagttatt ataagtcttt
     6001 acggacaatt atgcaatatt tgaacggcta taaagagctt attgcaacac ataataaaaa
     6061 tttacaggct ctgatttcac aacgacagac agaacgaaag aagtatgaac aggcacggct
     6121 tgatctacag tatttgatta atactgaaaa cagcattgca atggaaaagc ttacactact
     6181 gcaactgaag gtgggtttgc ttggttatta tattgactat aaagacttaa ctgaataaaa
     6241 taagtaaaaa gaatttccct tgcattctgt aattaaaact cttttaattt aatttttata
     6301 ggagttcata tatgtataca ataacaccca cgtattttta cagcaacaat tataaattag
     6361 atgcaagact gtatcttccc gataccggta aaaaaactcc tgttatcgtt atggcacatg
     6421 gctttggtgc ccgtatggat tggggtttgc atccatttgc tgatatcttt gcacagatgg
     6481 gatgtgccgt gttgatgttt gattatcgtg gttttggcaa cagtgaaggc aatattcgcc
     6541 gactggtata tcatgttcat catattgaag attatcatat ggctattcaa tatgccatga
     6601 caattcctaa tattgacagc tctaaagtgt tattatgggg cacatcattc agcggagggc
     6661 atgtattgac tgtagcatca caggatagct ctatagcagg ggttatagct caggtgcctt
     6721 ttgttgatgg tgttgccacg gcttttcatt tacctgttaa aaatatatta agcggcctgt
     6781 ggtatggtag cctggatgta gtgatgtctt taataacgtc aaaaccagtt acaataccac
     6841 tggtagccac ccctgacaca tttgctgcta tgaatacacc ggaatgttat gatggctata
     6901 tgaaactggt accacctgaa gcacgtgatg aaaattggtg ccccgccagg gtttgcctta
     6961 ctcttccatt gtacaggcca atagcagtag tggataaaat ttcatgtccg gtatgtatta
     7021 ttggggcaca gcacgattca ctcattccaa tcaaggcggt agaaaaaaca gcaaaaagaa
     7081 ttaagcacgt tgatttccac atattaccct gtggtcactt tgacccatat gatggagaga
     7141 tgtttgataa aaatatacag atacagaagc aatttttgca aaaagttttg taaaaatatt
     7201 aatacctgtc tcaaggatac aggtattaac gctaaacctt gcaaaataag caagtaaaat
     7261 tgccattgca ttcagattag tgtgactttt tttgcctgaa tattattttt ttggtgtttt
     7321 ttgtgaataa taattattag tattataatg catgcaataa atatgttaat tagaatatac
     7381 taaataaaat taaaaatatg ataacatagt agcaataaac acttgacata taatgttgtt
     7441 atactataat tagcttcact tttttttata aattcattca aagcataaaa gtacactatt
     7501 atatcactgt ctacagcagt ggggtgtaca taggtaattt taaaattatt ttagataatt
     7561 attgcaggag gtttgcatgt tgggattagg tgatgtatgg gtttttatgg cttacctgtt
     7621 gtgtattgca agtgcaattc tttgcgcagt gtatggtttt ataaaatgga atgacgatga
     7681 agagccatat accgatgaag caaaacgctg ggtacaggaa gaagccgaaa tagaaaaaac
     7741 actttaaact aacgaggtgc gtcatgacta tattctgggt atttgtatat cttgtaattg
     7801 ttgcattttt agggtatttg ggataccgcc atacaaaagg tgcttcagac tatcttgttg
     7861 caggacgaaa ggtacatcct ttcatcatgg cactatcgta tggagctaca tttatcagta
     7921 cttcagcaat cgttggattt ggaggtgctg ctggtgtttt tggtatgggg ttactgtacc
     7981 ttactttttt gaatattttt gttggaatat tcattgcatt tgtattcttt ggaaaacgta
     8041 cacgacgtat ggggtatcat ttagatgcac atacgtttcc tgagttttta ggaaggcgtt
     8101 tccaatcacc gtttgtacag aaatttgcgg ggattataat ctttttattt atgcctctgt
     8161 atgcagctgt agttctcatt ggtgcggcaa aatacattga acagcagttt gcaatagatt
     8221 atgccacagc gctactgttt tttacattaa ttgtagcagt atatgttatt atgggtggcc
     8281 tgaaaggagt aatgtatacc gatgccttcc agggatccat aatggctatt ggtatgttaa
     8341 cccttattat ctttacatac tacgatttag gtggtattat caaatcacat gctgatttaa
     8401 ctgttcttac acctccgcca ccactggctg caaaaggaca tatgggatgg acatccatgc
     8461 cagcgttagg ttctgatttc tggtggacgc tggtttcaac aattataatg ggtgtgggta
     8521 ttggagtgct tgcgcagcca cagcttgttg tacgctttat gacagtaaag agtaaccgtg
     8581 agctgaacag agcggtagca ataggaggaa tctttatact ccttatgacg ggcgttgcat
     8641 ttacttccgg tgcactttcc aacgtatggt ttacaaagaa tgaaaaaggc gtcaaatact
     8701 gtgaaatcag tggggcaatt ttagaagttt gccctggcaa gaaggttgta aaaattgatg
     8761 aaaagaatat tgaaaagttt aaagctcaat tcaaggacat aaaaccaggt gatgagattg
     8821 acctccataa aggtattcct cataaagaaa ccactaaggg ctcgctggca atagtagctg
     8881 cgggcggtgt ggttgaaaac attattccgc aatttgtgaa acaggcaatg ccacactggt
     8941 ttgcggtatt atttatgctg acattactag cagctgcaat gtcaacgctg agttcacagt
     9001 tccatgcaat gggtactgca atagggcggg acttctacat gcagatattg aaagataaaa
     9061 aagctgcaga agaacgaagt gtgtttatca acaagatggg cattggtgtt actattcttg
     9121 ttagcttaac gttagcatat tttctgccgg tgatatattc caaaggtgaa gctattatag
     9181 cacggggaac ttctattttc tttgggttgt gtgcagggac attcttgcca gcatatataa
     9241 gtgctctgta tttaaaacga attactaaag ctggtgtggt tgccgggatg ctgtcaggat
     9301 ttactgttgc atttttctgg ctggtgtttg tgcttgaaaa agaagctatg gcaataggtc
     9361 tgtgccaggc attgtttggg aaacccacac tgctttcgtc accatggaca atggttgatc
     9421 caatgtttat ttctttccca acgtcacttg ttgtaacact tgttgtttca ttgtttacaa
     9481 agaagcttcc tgaagaacat gttacacatt gttttcaggg cttagaaaaa taactttatc
     9541 tacataaaga tttcattagc attgttttct tgtacagggg ctgtcccaaa agaggtcgtt
     9601 gagtaaattt tgcaaacatt atatcgtaat actcgctcat ttcgatacaa ttttgccaca
     9661 aaatctggca aaattactca atgagcgttg cgaaatttgt atcgaaacga cttttgggac
     9721 agcccctata tgttatgggt attgctatgc taactgctat tcaaaataat tcatattatg
     9781 attctggtag cacgtctatg tgcttgccgg taaaacttgc aattttgtca tttatttttt
     9841 ctacctgtct ctggcttctt tcgtagcttg tcagtgcatt acgaagatgt ttccctgtta
     9901 tactgtattc atcttcaaag ttttttagtt ggttttgaac cgttgccaat ccatttaata
     9961 tgtctttagc tttctgttca atttggagtc cttttaaacc ataggctata gccattaaat
    10021 atgcataaaa actattaggc gatacaggta taactttgct tgcaagggca tagttgaaaa
    10081 tttcataggc tttatcgctt accgtatcat tgacaattat ttcataaaat acgttttctg
    10141 caggtatgta catgagtgca aaatcatacg ttccttcatc agggtttata tatttttgac
    10201 tgatttcgtc aatacgctgt tttaccgctc ttataaactc ttttttatac tggttttttt
    10261 cattatctga tgttgctttg ataattctct gaaaactttc aagaggaaat ttggaatcaa
    10321 cgggaataag ttgaccacca agctgaataa ttgcatcaac tttagtgcca tttttaaatg
    10381 tatattgcat gtggtaattt tttgcaggca aaaattgctt taatagttct tcaagcaagt
    10441 actcacccag gttgccgcgt aactttggtg cagcaagtat atcctgcagt gaggcaatat
    10501 cattgcctat ttcccgtatt tggtgtgctg tttgttcaag ggaacctaat ttggtgtgta
    10561 tatcgctcac aactttgtcg gtgttgttaa gttgttgggt aatgtttttt tgtgtcgatg
    10621 taagaatttc gttcactgta gaaataagct gtgataagtt tctttgttga ttttccagtg
    10681 ccgtattata cgcttcaagt tgtttggttt tcaattcaac aatctgattt tttaaatcaa
    10741 ttaattgaac ggctaattca ctggaagtat cgccttttgc aaagaatagt ttgaatgcaa
    10801 ttaacacaag aagtattact ataaacacca gtgcaatggt agtgtattcc atagtcaatc
    10861 cccaaaaatt gattttcaat aattactatc gccaggcact atacaatgac gcaccacacg
    10921 ccatatatgg taacccagcc tgtcaataaa tcgtattgct tcaatataat ccagtgcggt
    10981 atgggaatca atttgacctg ttgcagtttt tttaagaact tccacgcgct ccagtttgcg
    11041 agaatttgcc aggtattgcg atagttcctg aatcttttca acactatcta ttgaactttc
    11101 tgcctttaat gctttttcag attccactaa ctctgtatta aaattccatt tcatatcgga
    11161 aaagtttgtg ctgtttttta aaagagaaac aatgtgtgct tcctggcagg cttcaatgat
    11221 gctgtttaag tgatcaatgg catgaagtac tgagatatgt tcatcataaa gctgtttttg
    11281 ctgtggtggg gtttttacct tgctcataaa atgttttgtt tcttcaagag ctatctgtgc
    11341 tgttttaagc cggtgagcta tatcggtgcc gtttttctgc agcatgtctt tgagtatttg
    11401 aataatatac agcgctatgt caaccaccgt gcgtcgtgct gtttcaaccg cgatggctgg
    11461 tgttgtggct atggcatcgt ctaaatgctg tgtaagataa aacctggtat cggtaaataa
    11521 tgtttttaaa aagcgcacaa acggggtaat gaatggaaaa aacagcaaca cgccaatgac
    11581 gttaaagagt gtatgaaaca ctgaaagctc tatttcagga actatcgcac caaaagcatt
    11641 acataaagaa gaaagtagcg gcgatagttg tggtaataac acaaacaata caagtgcagt
    11701 cactgtgtta aaaagaacat cagctacaat aatacgcctg gtatcgggag ttgctccgat
    11761 ggcggcaaaa agtattggta cagacttccc tgcgttcatg ccaataacaa gaacctgccc
    11821 ctgaaaaagg ctaatggtcc cgctgctcaa tgccaccagt gccagcacta ctgcagcgct
    11881 tgatgattgc atcaggattg taagtatgaa acctattgaa aatagaagca gcctgtcagt
    11941 aattacatgt cctgagatgg cagtaaaatt aattgctgcg gcaactgtgc cagcccctga
    12001 cgcataatgg taataccaaa gaaaagaagg ccaaacccgg ccagcaatga accaatgtca
    12061 gatcgatggt cagtatacaa tatttttaat atgacaccgc aggcaacaaa aagaagcgaa
    12121 aattgcccaa tgctgtattg aatgccaaac attgttacaa gccatgctgt ggcagtagtg
    12181 cctacgtttg ccccaaagat gactgccagt gcttgtggta tggtgatgag ccctgcgctt
    12241 acaaatccta ttgttgcaat ggctgttgca ctggatgcct gaatgatagt ggttaccagc
    12301 atgccggtga acagtgcctt aaactttcca ccagtgaggt ttgcaagaat ttttctgaaa
    12361 cgaggacctg ctaatttctg tattgcttcg gacatcagga caatgcccag aaggagtatc
    12421 cctataccgc caattgctgt aattatatct gaaatattca tactggctta tctttgtgat
    12481 aattatcggc attatggtat gtaatcttcc cgtacggggg aaaatacttc aattgctatt
    12541 gagtcttcca gtacttctgc tccgtgttct atattacccg gtattgacca gcttccacct
    12601 ggtttcatgt catatgtttg tccatccata atcaggataa tatgcccttt taccaggtac
    12661 cccgtttgct catgagggtg tttgtgaatg ggcaatttct ttcctttttg taatctaaac
    12721 tcggtcatta atgtatgggt accaaagaca agagttttgc gctcaatgcc atcaagcacg
    12781 gtcatatatc catcaggtga aggattggc
//
LOCUS       DAORYL010000086        12647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_3897_length_12647_cov_13.082274, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000086 DAORYL010000000
VERSION     DAORYL010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12647
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3897_length_12647_cov_13.082274"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PLV81_12785"
     CDS             <1..789
                     /locus_tag="PLV81_12785"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOM11015.1"
                     /translation="IVEKCGYFIFFGIAGALVLISGIAGGIAAIEPQRAIVPVREPLL
                     KRIKQSLNTQALRQNKNLLYVLLAIGFWGMAFNVFFPYLLIYLKHFLKVDIATSSVLI
                     AISIFVGGILASLPAGVLADKIGRKPIAVFAVILISASLIAFSFARSPITIGILATMW
                     IIAQTIWMTATGAWSKDLFPEERRGEFAGYFTLFYVAFTMIPGPLIGAVVADRWGIRQ
                     IIDGKPGIIPTPEIFQVSAILILITIFPLLFAKELHNATTAIGK"
     gene            complement(995..1615)
                     /locus_tag="PLV81_12790"
     CDS             complement(995..1615)
                     /locus_tag="PLV81_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11016.1"
                     /translation="MRNASIALLSVLTVTMLLMFNISYLPAQNTMLFLSPEFAKLVCD
                     AWNQSELPQKLGTKEAGGNNWILTENKYTGEVRNKQVLVMSRRDCSAMPKVQLTIENK
                     NGKALCTYGGALTESYEKAEWAFAPTTVQWYKFASGIWGYMQMPGIMKGFRGPMFVAR
                     ANIDNFGTFWKIVGNVAKQTNADYKTNCNLTSDDVEDIEEYLKKIK"
     gene            2024..3121
                     /locus_tag="PLV81_12795"
     CDS             2024..3121
                     /locus_tag="PLV81_12795"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOM11017.1"
                     /translation="MKRIITIGIITIIILLSGFIAYLLLTDYILIRYPVLVQTNPKIT
                     FIIGDAYFRESPKAQWKVAVVGQTLYKGYEIKTDPDSRMDIRLHDNTAIQLSGNSEIS
                     IDSLTVKKLSLNIHAGSLYGKFEKLFKEHDLQVKTPTAFAAIRGTDLGFEVIPVQEDE
                     TAQAGKKKQAQNKTIKKTQNNKIQPESTMQTVVYSLSGITEVYNPLFDDQKVLLSYRN
                     KVLIDKDKMPNNPEELTEDEIRSLQEKLNSLHFDEVLLITDKITFELGSAEIQPSSYP
                     ELDKIVEILKQKKVDVRIEGHTDDIGEAAFNQLLSEKRAESIKNYLVSKGLDPKRFQT
                     VGYGESKPIADNKTEKGRAQNRRVEFLIIEK"
     gene            3177..3259
                     /locus_tag="PLV81_12800"
     tRNA            3177..3259
                     /locus_tag="PLV81_12800"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3211..3213,aa:Ser,seq:tga)
     gene            3268..3354
                     /locus_tag="PLV81_12805"
     tRNA            3268..3354
                     /locus_tag="PLV81_12805"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3302..3304,aa:Ser,seq:gct)
     gene            3371..3799
                     /locus_tag="PLV81_12810"
     CDS             3371..3799
                     /locus_tag="PLV81_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11018.1"
                     /translation="MYLQHIKKYSAILYATITEYCTHVKTFCIEHKREIILSIAYVPY
                     IGWLYPLYAFEDAQVQKHAKRGLLYAMTAVAIALTIFLIVFMMPRPWRLLRFVFTILL
                     YLNYFVYYAIVTTHIYCIWNKKDFSIPQVENYLEKLEQYI"
     gene            3822..3895
                     /locus_tag="PLV81_12815"
     tRNA            3822..3895
                     /locus_tag="PLV81_12815"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3856..3858,aa:Arg,seq:acg)
     gene            complement(3967..4242)
                     /locus_tag="PLV81_12820"
     CDS             complement(3967..4242)
                     /locus_tag="PLV81_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11019.1"
                     /translation="MQIHIGFADTFIFSPFLMMKSMQGSTDITTDPAYVGMQTSYDIP
                     STTMISYGFDIIETTSGVTLSGLINMAGKTNETAGYNSYIFGVSIKL"
     gene            complement(4279..4755)
                     /locus_tag="PLV81_12825"
     CDS             complement(4279..4755)
                     /locus_tag="PLV81_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11020.1"
                     /translation="MKNKVTLYFCFLFILSASCLFAQSSSDTQTNFSISPPPLGYPEY
                     PQESSFSNTMNFTGAYISADQFSMVGMFLGWVPRYGGDWGAIEGMLGMFIMNGEIDTG
                     FGKQSLNFVGFDMTPAFELPIVNQESFKFIFFGGYDLMLMTTSFTTTDPYSSGTMT"
     gene            complement(4899..5966)
                     /locus_tag="PLV81_12830"
     CDS             complement(4899..5966)
                     /locus_tag="PLV81_12830"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOM11021.1"
                     /translation="MKKSYNIAVLPGDGTGPEVVAEGVKVLKAVAQVENITLNFTNYD
                     FGGERYLKTGETLPPGAIDELKKYDAIFLGAIGHPDVKPGILEKGILLTLRFELDQYI
                     NLRPVKLYPNVETPLKDKGPQDIDYVVVRENTGDVYTGIGGVTMKGTPHEEAMQIMVY
                     NRFQVDRCLRYAFEYARKRNKKKTLALVGKTNVLTYVYDLWERAFHEIGEKEYPDIKR
                     EYYHVDATCMWMVKNPEWFDVLVTGNMFGDIITDLGAITQGGMGIAAGGNINPEGVSM
                     FEPIGGSAPKYTGKNVINPLAAICAAQMMMAHLGEESAANRIEKGVVEVLQRDMKSLA
                     AGKMGLTTTQVGDKVAEYAVK"
     gene            complement(6392..7078)
                     /locus_tag="PLV81_12835"
     CDS             complement(6392..7078)
                     /locus_tag="PLV81_12835"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOM11022.1"
                     /translation="MKITTVIIRLLVLASPLLLNDLYLPLVPKDATTLSIVLDVLVYI
                     GWQTTLLYVAYEAGWFSWNSLGINLTQVKQWLWGVVLFVCVFVAYTGITIAFMFAKKY
                     YGITVESFWYFPLPQWHGVYVFLYVLYLSITAGIYEEIIYRGIAIHQLKNLTSNTGIL
                     VIVSTLLFVAIHWSMGPGTWMEAGLWGALWAYLYIKTHSLLPIMIAHVLYDVASIYGL
                     HEAIAHSIGL"
     gene            complement(7097..8065)
                     /locus_tag="PLV81_12840"
     CDS             complement(7097..8065)
                     /locus_tag="PLV81_12840"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOM11023.1"
                     /translation="MSLSIPYLVSGGIITSYSCSSRCRHCLYGCSPQRDRTYIHADTL
                     NTVCAVLLKNGCTSVHVGGGEPFLQIEKLLEAIDIITRHGIDIEYIETNASWYNNNKK
                     HVLHNLKEHGVHTLLISISTFHNEFVPFSKTKELIAACNDSGIHVFPWVMDFYKDLSV
                     FDSSCTHSLEEYESYFGKGYVASIPGRYWIHYGGRALKLFKGYYRTKLLEEILASSPC
                     REITNTSHFHFDVYGNYIPGLCSGLAIQADDIGKEIDSITYPFITTLYSEGVAGLYRL
                     AQQEGFVADSSYLNKCDLCLHIRTFFVTQKKMNTPELQPVEFYYNT"
     gene            complement(8067..9404)
                     /locus_tag="PLV81_12845"
     CDS             complement(8067..9404)
                     /locus_tag="PLV81_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOM11024.1"
                     /translation="MKLRSLFAPPPPKPLLPAEKVNKLYPKSRWNILEATFIGYATFY
                     IVRNNLSTVAKDMQAVFGYDHSQIGSILAVSAFAYGLGKFLLGSVSDRSNVRTFMPFG
                     LVLTALLNFAFGACHNYTIHFWLWALNGFIQGAGWPPCGRSIGHWFSVSERGTIFSIW
                     NIAHNVGGGIAGVLAAYAASHFGLQNAFFIPGTIALICAVYLLWRLRDTPQSVGLPPI
                     EEYRNDYPPAAPLHCEVELSTKVLFVDYILKNKLLWVVAFANFFVYIARYSMLDWGPM
                     FLREVKGANITSGGFSVMILEFAGIPSTILFGWISDKLQGRRGMVSLLCMIPVVLAFY
                     AIVVYPHTSIWFYYAMFSVIGFFVYPPVMMLGVMGLDLTNKKAVGTAAGFIGMFGYLG
                     RTAQAKGFGVILDHFSTTLDIVSRWNIVLYTIVACTVLSIVLLAFTWKVKPRG"
     gene            9685..10296
                     /locus_tag="PLV81_12850"
     CDS             9685..10296
                     /locus_tag="PLV81_12850"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOM11025.1"
                     /translation="MKITKKRKDGLKRQSEIMLIALELFSKKGYHDTKLEDILKKAKI
                     ARGTFYLHFNGKDDLLHMIVDYHLEEMYRVISVLDISMDKPIEEISSLYITIAQKLIN
                     DKRFKMFVRIMLKEAIGLDKTLLQKLNNFYNKIITMSAEYITKAQQKGRILSSIDPLF
                     TSMCIIGSIKELVFQWAVNDDSLDIEKGILTAITVYFRGMINS"
     gene            10397..10756
                     /locus_tag="PLV81_12855"
     CDS             10397..10756
                     /locus_tag="PLV81_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11026.1"
                     /translation="MKLSEIKDLLNADVIVGEEMLNSVEVDTAFASDLLSDVLAYAKE
                     RALFITGLTNPQVIRTVEVLDLIGVVFVRGKVPQMDTVELAKRKNIPLLRTKCIMFET
                     CGRLYGAGIKASIEKVD"
     gene            10765..11571
                     /locus_tag="PLV81_12860"
     CDS             10765..11571
                     /locus_tag="PLV81_12860"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOM11027.1"
                     /translation="MQYTQCANHIFSYTLRGGDFDNAGRISTSLKRELRTREYPPHII
                     QRVAIVLYEAEINVVSYTKVGYFFAYCRPNSVHLVIKDKGKGIENIKLAVQEGFSTAT
                     DEIRRLGFGAGMGLSNIKRNANVMRICSKPGEGTKISIDITSSNHYGDLIMDITVNEI
                     QQKTNFELLTSKADCSKLITGGYVGDMLSDVNANGKKGNIWITILTHPNAVAVASLKD
                     FSAIVVAGGIKPREEFIKRAEEEDIPVLYTDLSAYEVVVLLSSLGVTANH"
     gene            complement(11608..12330)
                     /locus_tag="PLV81_12865"
     CDS             complement(11608..12330)
                     /locus_tag="PLV81_12865"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOM11028.1"
                     /translation="MDSNNKYFERFSRTSLLIGHDKLKKLLTSTVYIAGLGGVGGYAF
                     EAIVRAGIGTVHVIDFDIVDISNCNRQILASDSTVGMPKVDVACHRAQDINPDIIIHK
                     HHIQITPDNVATLFQSHVDFFIDAIDDVAAKVALIEYLHAHTIPFISCMGAARAFDPL
                     SLRISDISKSQYCPLARVIRKRLKEREIYKGVPCVYFHEERLSNRADSDNMSLPGSLS
                     YMPGIMGLAAAGFAIKHLIGNV"
BASE COUNT         4151 a         2484 c         2281 g         3731 t
ORIGIN      
        1 attgttgaaa aatgtggcta ttttattttt tttggcattg ctggtgcact ggtgcttata
       61 tcaggcattg ctggtggcat tgcagcaatt gagccacaaa gggcgatagt tcctgttaga
      121 gagccattgc tcaaacgaat taaacaaagc ctcaacacac aggcactcag gcaaaataaa
      181 aatttattat atgtattgct tgccattgga ttctggggaa tggctttcaa tgtgtttttc
      241 ccgtatttac taatttatct gaaacatttc ttaaaggttg atattgcaac atcatcggta
      301 ctgattgcca tttcaatatt tgttggaggg atattagcgt cgctgccagc aggggtttta
      361 gccgataaaa ttggtcgtaa acccatagca gtgtttgcgg taatattaat ttcagcatca
      421 ttaattgcat tttcgtttgc acggtcacca ataactatcg ggattttagc taccatgtgg
      481 ataatagctc agacaatatg gatgactgca acaggtgcat ggagcaagga tttattcccc
      541 gaagagcgca gaggcgagtt tgcagggtat tttacgttat tctatgtagc gtttaccatg
      601 atacctggac cacttattgg cgcagtagta gctgacaggt ggggcatcag acaaattatc
      661 gatggcaaac cgggcatcat cccaacacca gaaatttttc aggtttcagc tattttgatt
      721 ttaattacca ttttcccatt gttatttgca aaggaattac ataatgctac aactgcaata
      781 ggaaaatgat gacaccatag caccaatctc aataataaca atatcttctt taatgagata
      841 gatttaactc taccttctcg atgagtacat tcaccatcct gtcatttcga ggagcatagc
      901 gacgagaaat cttttctact gacacatacg attcctcccc ctttcagggc tcgaaatgac
      961 agcagtgaat gagaccgcca cacttaatca ctgtttattt tattttctta agatattctt
     1021 ctatgtcctc aacatcatca cttgttaaat tgcagtttgt tttataatcg gcattggttt
     1081 gctttgcaac gttgccaact atcttccaga atgtgccgaa gttgtcaata tttgcacgag
     1141 ccacaaacat ggggccccgg aaacccttca tgattccagg catctgcatg taaccccaga
     1201 tacctgaagc aaatttatac cactgtactg ttgtaggagc aaaagcccat tcagcttttt
     1261 catagctttc ggttaatgct ccaccatagg tgcatagtgc ttttccattt ttattttcaa
     1321 tagtcagctg aaccttaggc attgcactgc aatccctgcg tgacattacc agcacctgct
     1381 tatttcttac ttccccagta tacttgtttt cagtaagtat ccagttattt ccgccagctt
     1441 ctttagttcc caatttttga ggcagctcac tctgattcca ggcatcgcat accagttttg
     1501 caaattcagg gcttaagaaa agcattgtgt tctgggcagg caaataggaa atattaaaca
     1561 tcagtaacat tgtaacagtt aatactgaca gtaacgctat tgaagcattt ctcatatatt
     1621 cctccgatag tataaatatt aatacatagt tatattgcag ccttaaatat gtcaattatt
     1681 ttataatatt attaattggt ataatacttt acaacctaaa actaatgtat tgatccatta
     1741 tcacgattaa tatacatcac cagaatttat ctcttccaga ggaactgatt tcaaagtatg
     1801 aaaatcccca ctatttattt ctaaatttaa tgtatacgtg aaattaatcc tcatcttcat
     1861 aaaactgaaa aagtgtataa tgatcttttt aaatttttaa taattgactt ttattatatt
     1921 atgtactatt cgttcatgaa atagtcatgt atagattatt tacaatatca ttataaaatt
     1981 cctaaataat caaaaaattt atttaccata atggagaaaa tatatgaaac gaattattac
     2041 aataggcatt attactatta ttattctact ctctggtttt attgcatatc tattattaac
     2101 agattatata ctcatccgtt accctgttct ggtacagaca aacccaaaaa taacatttat
     2161 cattggtgat gcatactttc gtgaaagccc taaggcacag tggaaagtag cagttgtggg
     2221 acagacactg tataagggct atgaaataaa aactgatcct gattcacgga tggacatacg
     2281 gttgcatgat aatactgcga ttcaactatc gggaaattct gaaatatcaa ttgattcatt
     2341 gacagtaaaa aaactgagcc tgaacatcca tgccggttca ctatatggaa aatttgaaaa
     2401 attatttaaa gaacatgatc tccaggttaa aacaccaacc gcctttgctg ctatcagggg
     2461 aactgacctg ggttttgaag ttatcccggt tcaagaagat gaaacagccc aagcaggtaa
     2521 aaagaaacaa gctcaaaaca aaacaatcaa gaagactcaa aataataaaa tacagcccga
     2581 atcaacaatg caaacagtag tatattctct ttcagggatt accgaagtat ataacccatt
     2641 atttgacgat cagaaagttc ttctgtcgta caggaataag gttttaattg ataaagacaa
     2701 aatgcccaat aatccagagg aattaaccga agatgaaata cgatcactac aggaaaaact
     2761 caattcactt cactttgacg aagtactcct tatcacggat aagattacct ttgagttggg
     2821 tagtgccgaa atacagccat catcatatcc tgaactggac aaaattgttg agatattgaa
     2881 acaaaagaaa gtggatgtcc gtattgaagg ccataccgac gatattggcg aagcagcttt
     2941 taaccagtta ctatcggaaa aaagagcaga atcaatcaag aattatcttg tttcaaaagg
     3001 gcttgaccca aaacgattcc aaactgtggg ttatggtgaa tcaaaaccaa ttgccgacaa
     3061 taaaaccgaa aaaggaagag cccagaacag gcgggtagag ttcctcatca tagaaaaata
     3121 attgcactgt aaaattttta tttgacaaca accgcccttt caatagtgtt gcactcggaa
     3181 ggttgtccga gtggtctatg gaggcggtct tgaaaaccgt tgggtgaaag ccccgggggt
     3241 tcgaatccct caccttccgc atgaaacgga gagatgcccg agaggccgaa ggggacggtt
     3301 tgctaaaccg ttgtactgga aacggtaccg agggttcgaa tccctctctc tccgaatgga
     3361 aactctaaga atgtacttac agcatattaa aaaatacagc gctatcctgt atgcaacaat
     3421 cactgagtac tgtacgcacg taaaaacatt ttgtattgaa cataagcgtg aaattatttt
     3481 atccattgca tatgtgccgt acattggatg gctgtatcct ctctatgctt ttgaggatgc
     3541 gcaggttcaa aaacatgcaa agcgtggact tttgtatgca atgaccgcgg ttgctatcgc
     3601 cctaactatt tttttaattg tctttatgat gccacgccca tggcggttac tacgttttgt
     3661 atttacaatt cttttgtacc ttaactattt tgtatattat gcgatagtta ctacgcatat
     3721 atactgtatc tggaataaaa aagatttttc aataccacag gttgaaaatt atcttgaaaa
     3781 attagagcaa tatatctaaa ttttagatat agcttttaca tgcgtccata gctcagttgg
     3841 atagagcgtc ggactacgaa tccgaaggcc agaggttcga atcctcttgg gcgcacaaat
     3901 aaaggggctg ccatataaaa gcagccccgt tgtaattata cgctaaactt tctgctcaaa
     3961 agaatactac aattttatgc tgacaccaaa tatatacgaa ttataccctg ctgtttcatt
     4021 ggttttgcct gccatgttga taagcccgct caatgttacc cctgaagttg tttcaataat
     4081 atcaaaacca taagaaatca ttgtggttga aggaatgtca tacgacgttt gcataccaac
     4141 atatgcagga tcagttgtta tatcagtact tccctgcatg gatttcatca tcaggaatgg
     4201 cgaaaaaata aatgtatcag caaatccaat atgtatctgc attccaagga ctggaccata
     4261 cataactgtg gttaatgttc atgtcattgt tccactacta taggggtcag ttgttgtaaa
     4321 tgaagtggtc atcagcatca aatcataacc accaaaaaag ataaacttaa aagactcttg
     4381 gttaacaatt ggcaattcaa atgctggtgt catatcaaac ccaacaaaat tcaatgattg
     4441 cttgccaaaa ccagtatcaa tttcaccatt catgataaac atacctaaca taccctcgat
     4501 ggctccccaa tcacctccat accgtggaac ccatcccaaa aacatcccaa ccatggaaaa
     4561 ctggtcagca ctgatgtatg ctccagtaaa attcattgtg tttgaaaaag atgactcttg
     4621 tggatattcc ggatatccta acggaggagg ggaaatagaa aagttggttt gcgtatcgct
     4681 gcttgattgc gcaaacaaac aagaagcaga aagaataaaa agaaagcaga aataaagagt
     4741 aactttgttc ttcatggttt tatctccaag attaggttca tgtaaattgt atagatattg
     4801 caatttctaa agtcaatagt tttttgtttt aaaaaaagca atagcgatga tttgataatc
     4861 accgctattg cagccatcga cttatataaa aatttgctct acttcaccgc atattctgct
     4921 actttatcac caacctgtgt tgtggtaagg cccatcttgc cagcagccag ggacttcata
     4981 tcacgctgca atacctcaac cacacctttt tcaatacgat tggctgcact ttcttcaccc
     5041 agatgtgcca tcatcatctg tgctgcacat atagctgcaa gcggattaat aacattttta
     5101 ccggtatatt ttggtgcaga accgccaatt ggctcaaaca tagaaacgcc ttcaggatta
     5161 atattaccac cagcagcaat ccccatgccc ccctgcgtta ttgcccctaa atcagtaata
     5221 atatcaccaa acatatttcc agtaacaagc acatcaaacc actcgggatt cttcaccatc
     5281 cacatacagg tagcatcaac atggtagtat tcacgcttaa tatcgggata ttctttttca
     5341 ccaatttcat gaaaagcgcg ttcccataaa tcatacacat acgtcaatac attggttttt
     5401 cctaccagtg ccagtgtttt ctttttattg cgcttgcgag catattcaaa cgcatagcgt
     5461 aagcacctat caacctgaaa acgattatac accattatct gcattgcctc ttcgtgaggg
     5521 gtacctttca tggtaacacc accaatgccg gtatatacat cgcccgtatt ttcacgcacc
     5581 acaacataat caatatcctg cgggccttta tctttaagcg gagtttctac attgggatac
     5641 aacttcacag gacgtaaatt aatatactga tccagttcaa aacgcagcgt aagcaatatt
     5701 cctttttcta aaatacctgg cttaacatca ggatggccta tagctcctaa aaaaattgca
     5761 tcgtattttt tgagttcatc gatagcacca ggaggtaatg tttccccagt ttttagatac
     5821 cgttcgccac caaaatcata attggtaaaa ttaagtgtaa tgttttctac ctgagcaaca
     5881 gcttttaata ctttgacgcc ttcagcaaca acttcagggc cagtaccatc gcccggtaac
     5941 acagcaatat tgtagctttt tttcataatg cattctccat tagttaatat attatattct
     6001 atgatattta aacaagaaaa cgttttaata ttatttttaa tcaacaatta caattataaa
     6061 ataatagcgc aagtttttct ataattcata tcaaatattt attaccacaa tatttatttt
     6121 aagcgaaagt tattaatact tattcgatag tatgttaata aaacaatttt gatgatagta
     6181 atttgaatac cattaaaaaa tcttatcaaa aattaacaac tcaatcgatg aacgtaaaaa
     6241 tactgcagta gagattgtta aagcaattta ttacctgaat aacataatgc atgacgtatt
     6301 agagcatgat aaaaatcttg ttagttctat gaaaactctt gaagagttaa cagtaaaact
     6361 ttccgagatg tctaagtgat atgttacgat atcaaagacc aatactatga gctatcgcct
     6421 catgtaatcc gtatatagat gcaacatcat ataaaacgtg tgcaatcata attggcaaaa
     6481 gcgaatgcgt ttttatgtac aggtaagccc acagcgctcc ccataaacca gcttccatcc
     6541 aggtaccagg acccattgac cagtgaatgg caacaaacaa aagcgttgaa acaatcacaa
     6601 gtatgcctgt atttgatgta aggtttttta gctgatgtat ggcaatgcca cgataaatga
     6661 tttcctcata gatgccagca gttattgaaa gatacagcac atataaaaac acatacacac
     6721 catgccattg gggaagggga aaataccaga atgattcaac cgtaatgccg taatattttt
     6781 ttgcaaacat gaatgcaatg gtaataccag tgtaggcaac aaacacacag acaaaaagaa
     6841 caacacccca taaccattgc ttgacctgcg ttagattaat acctaaactg ttccatgaaa
     6901 accacccagc ctcataggcc acatataata gtgtagtctg ccagccaatg tacaccagca
     6961 catcaagtac tatagaaagc gttgttgcat cttttggcac aagcggcagg tacaggtcat
     7021 tgagcaaaag cggggatgcc agcacaagaa ggcgaataat aacagttgtt attttcatga
     7081 ttggattcct taacaattat gtgttataat aaaattcaac aggctgcaat tcaggagtgt
     7141 tcatcttttt ctgtgtaaca aaaaatgtgc gaatatgcaa gcacaaatca catttattta
     7201 aataactact gtcagcaaca aaaccttcct gctgcgccaa tctatataat ccagcaacac
     7261 cttcagagta tagcgttgtt ataaaaggat atgtaatgct gtcaatttct ttaccaatgt
     7321 catccgcctg tattgcaagc ccactgcaaa gaccaggaat ataattgcca tacacatcaa
     7381 aatggaaatg actggtattt gtaatctcac gacatggcga gcttgcaagt atttcttcaa
     7441 gcagttttgt gcgatagtaa cctttgaaaa gttttaatgc cctgccacca tagtgtatcc
     7501 agtacctgcc agggattgac gcaacatatc ctttaccaaa atacgattca tattcttcca
     7561 gagaatgagt gcaggaacta tcgaacaccg ataaatcctt ataaaaatcc attacccacg
     7621 gaaagacatg tatcccgcta tcattacagg cggcaataag ttcctttgtt tttgaaaacg
     7681 gtacaaactc gttgtgaaat gtgcttatag atataagcaa ggtatggacg ccatgttctt
     7741 taagattgtg caatacatgc tttttattgt tgttatacca cgatgcattg gtttcaatgt
     7801 attcaatatc tatgccatgg cgtgttatga tatcaatagc ttctaaaagc ttttcaatct
     7861 gtaaaaacgg ttcacctcca ccaacgtgaa cactggtgca accgtttttc agcaaaacag
     7921 cacacactgt attgagcgta tcagcgtgta tgtaggtcct atcacgctgt ggggaacagc
     7981 catacaggca atgcctgcaa cgcgaagaac atgagtagct ggtaatgatg ccgccagaca
     8041 caagatacgg aatggataac gacatgttaa cctcgcggct taaccttcca tgtaaatgct
     8101 aacagtacaa ttgagagcac tgtgcatgca acaatagtat acagcacaat attccatcgg
     8161 ctgactatat caagagttgt actgaagtgg tcaaggatga cgccaaagcc ctttgcctgt
     8221 gctgttcgtc ccagatagcc aaacatacca ataaaacctg ccgctgtgcc tactgctttt
     8281 ttattggtaa ggtcaagccc cataacacca agcatcataa caggcggata cacaaaaaat
     8341 ccaataaccg aaaacattgc atagtaaaac catatgctgg tatgtggata caccacgatt
     8401 gcataaaatg caagcaccac aggaatcatg cacagtaaac ttaccatacc gcggcgcccc
     8461 tgaagtttgt ccgaaatcca gccaaacagt atggttgatg ggatgccagc aaactccagg
     8521 atcatgacgc tgaacccacc agaagttatg tttgcgcctt tgacttcgcg caaaaacatt
     8581 gggccccagt caagcatgct gtagcgggct atgtagacaa aaaagtttgc aaaggcaacc
     8641 acccataaaa gtttgttttt tagtatataa tccacaaaca gcactttagt tgaaagttca
     8701 acttcgcaat gaagaggtgc tgctggtgga taatcattgc ggtattcttc aatggggggc
     8761 aatcccactg attgtggtgt atcgcgcagc cgccacagca agtatacggc acatataagg
     8821 gcgatagttc ccggaataaa aaaagcattt tgcaggccaa agtggcttgc agcatatgct
     8881 gccagtactc ctgcaatgcc accaccaaca ttgtgtgcga tattccatat tgagaatatg
     8941 gtaccacgtt cactgacgct gaaccagtgg ccaatggatc ttccgcacgg aggccagcca
     9001 gcaccctgaa taaaaccgtt taatgcccac agccagaaat gtatggtata attgtggcag
     9061 gcaccaaatg caaagtttaa cagcgcggta agtaccaggc caaacggcat gaaggtacgc
     9121 acgttgcttc ggtcagaaac tgatccaagc aaaaatttac ccagcccgta ggcaaacgca
     9181 ctcactgcca gaatacttcc aatctgtgaa tgatcatagc caaaaactgc ctgcatgtcc
     9241 tttgcaacag tagataagtt gtttcggaca atgtaaaatg tagcatatcc aataaatgtt
     9301 gcttccagga tattccagcg cgattttgga tatagcttgt taaccttttc agcaggaaga
     9361 agcggtttgg gtggtggcgg tgcaaatagc gagcgtagtt tcatagtgag ttccttgtaa
     9421 tgtaacattt gttatgtatg aatggatgat atatgtatat ataagtaatt gtaaatatat
     9481 ttttaccgtt acgatctgat ttttcatcgc tgattattat aataaaaata taaaataccg
     9541 taaactccgt taatcataaa attttttcaa ggaataattc ataaacatat ataaaaaatt
     9601 attattgata tttaacacat gggaattgta tctattcact aatagaatat attacaatat
     9661 taatgagata ataccggcca tacaatgaaa attacaaaaa aacgcaaaga tgggttaaaa
     9721 cgacaatccg aaatcatgct cattgcattg gagttatttt caaaaaaagg atatcatgat
     9781 acaaaacttg aagatatctt aaaaaaggcc aaaatagcca gaggtacttt ttatctccat
     9841 tttaatggca aagatgattt attacatatg attgttgatt atcaccttga ggaaatgtac
     9901 agagttataa gcgtgcttga tatctcaatg gataaaccaa ttgaagaaat aagttcatta
     9961 tatataacta tcgcacaaaa acttattaat gataaaagat ttaaaatgtt tgtccgcatt
    10021 atgctaaaag aagcaattgg ccttgataaa acactattac agaaactcaa taatttttat
    10081 aacaaaataa ttacaatgtc agcagaatat atcactaaag ctcaacaaaa aggaagaata
    10141 ttaagctcta ttgaccctct ttttacatca atgtgtataa ttggatctat caaagaactg
    10201 gtatttcagt gggcggtgaa tgatgattca ttggatattg aaaaaggaat tttaactgcg
    10261 attactgtat atttccgtgg tatgattaac agctgatatt tattacttga ctaaaaagtg
    10321 atatttagaa ttgtatgaaa aaagtgtaca tattgttata tattcaacat cttaacaggg
    10381 aaaatgtaaa caggatatga aactatctga aatcaaggat ttgcttaatg cagatgtgat
    10441 agttggcgaa gaaatgctga atagtgttga agttgacaca gcatttgcat ctgacctttt
    10501 aagcgacgta cttgcgtatg caaaagaaag ggcattattt atcactgggc ttaccaaccc
    10561 acaggtaatc aggaccgttg aagtccttga ccttattggt gtggtatttg tacgaggcaa
    10621 ggtgcctcaa atggacacag tagaacttgc aaagcgtaaa aatattccgt tactcaggac
    10681 caaatgtata atgtttgaga cctgcggaag attgtacggt gcagggataa aagcatctat
    10741 tgaaaaagtt gattaaacta atatatgcaa tacacgcaat gtgctaatca tatattttca
    10801 tacaccttac gaggtggcga ttttgataat gcggggagga tatccacatc attaaaacgc
    10861 gaattgcgta cacgggaata tccacctcac atcattcaac gtgttgccat cgttttgtat
    10921 gaagctgaaa taaatgtggt atcatacacc aaagttggtt acttttttgc ctattgcaga
    10981 ccaaattcag tgcatctggt aataaaggat aaaggcaaag gtattgaaaa tattaaatta
    11041 gcagtgcagg agggtttttc aacagctacc gatgagatac gaaggcttgg atttggcgct
    11101 ggcatgggcc tatccaatat taagcgtaat gccaatgtca tgcgaatttg ttcaaagccg
    11161 ggtgagggta caaaaattag tatagatata acatcaagca atcattatgg agatttaatt
    11221 atggacatca cggtaaacga aattcaacaa aaaacaaatt ttgaactgtt gacctcaaaa
    11281 gccgattgtt caaaactgat tacaggtggc tatgttggtg atatgctatc tgatgttaat
    11341 gctaatggaa agaaaggcaa tatctggatt acaattttaa cacatcccaa tgcggtagca
    11401 gttgcttccc tcaaggattt cagcgcaatt gttgttgctg gcggcataaa acctcgggaa
    11461 gagttcatca aacgtgctga ggaagaggat attccagtgt tgtatactga tttatctgct
    11521 tatgaggttg tggttctttt gtcctcatta ggcgttacag ccaatcatta atgtaattat
    11581 tttatttttg gcgatagttc ctatacatta tacgttgcct attaaatgct taattgcaaa
    11641 accagctgca gccagtccca ttattcctgg catatatgac aaactaccag gaagactcat
    11701 attatccgaa tcagccctat tgcttagccg ttcttcatga aaataaacac agggaactcc
    11761 tttatagatt tctctttctt taagccgttt tcgtataaca cgtgctaatg ggcaatactg
    11821 gcttttactg atatcgctta tccgtaaact taaaggatca aacgctcttg cagcacccat
    11881 gcagcttata aatggaatgg tatgggcatg caggtattca attaaagcca cttttgcagc
    11941 aacatcatca atggcatcaa tgaaaaaatc aacatgactt tggaatagcg ttgctacatt
    12001 atcaggagtt atttgtatat ggtgtttgtg aataatgata tcaggattaa tatcttgtgc
    12061 tctatgacag gcaacatcca cttttggcat tcctactgtg ctgtctgaag ctaaaatttg
    12121 cctgttacaa ttggatatat caactatgtc aaaatcgata acatgaacag tacctatacc
    12181 agcacgtaca attgcttcaa atgcatatcc tccaacccca ccaagccctg caatatacac
    12241 agttgacgtt aaaagttttt tcagtttatc atgaccaata agcagtgaag tgcgtgaaaa
    12301 acgttcaaaa tatttattat tgctatccat tctcttttta ctcaagccat catatagttg
    12361 tattacgtca attatagcat atccctgtaa ttgcaattat atttcttttt agttttttta
    12421 agtacaatat aatgataccc agcaaattta acacagaaaa tcaatacaaa agctttctac
    12481 caattgaaag catactttgc ccatagctta tacggctaca caagatacta aaacaaatga
    12541 ttgaaaaaaa taataaagaa ttagtctttt tcaagcttgt gccagtatct caggtggtgg
    12601 tccccagtag aattttaggc aatggatgcc gttttcacgg taaaaat
//
LOCUS       DAORYL010000087        12607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_3910_length_12607_cov_11.702836, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000087 DAORYL010000000
VERSION     DAORYL010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12607
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3910_length_12607_cov_11.702836"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PLV81_12870"
     CDS             <1..272
                     /locus_tag="PLV81_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11029.1"
                     /translation="FAKDGFHYLLWLLWYLAYNPIRGRLCSDPTKYHYSSIRAYLEED
                     ADVGVPIDHHDYFVQLGKTFAERVVKFMWYEEYYRKKYSYVVDWV"
     gene            537..2408
                     /locus_tag="PLV81_12875"
     CDS             537..2408
                     /locus_tag="PLV81_12875"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOM11030.1"
                     /translation="MALNPLVDSRDVRFVLYEMMDVLQLTKLPKYSSFDKDMFEDMLN
                     LAETMAVEQIYPTFAEGDKEGCHYDGATKKVSIPKCYHAALDAFYEAGFGGMDVSDEW
                     GGMGIPQVISWACNEFFCAANYPLLMYPGLTHGAAELIEAFGTEEQKHMYLANMYSGK
                     WGGTMCLTEPDAGSDVGALKTKAVRQPDGTFKIYGQKIFISSGDNDYYENMIHPVLAR
                     IEGDPPGTKGISIFIVPKYRVNPDGSLGEFNDVTCAGIEHKMGIKGSATCTLSFGDNN
                     NCIGWLLGQERQGMKIMFKMMNYARMGVGLQAHGCASAAYMHAVTYCKNRVQGVHVTQ
                     MLNPEAKGVTIINHPDVKRMLLWMKSYLEGQRAMIYYLYLNYELAQLLEGEAAKEAQA
                     LVEIITPIEKAGCSDMSVLITSEAMQCYGGYGFVADYPVEQMMRNSKITAIYEGTNGI
                     QSMDLTMRKILMNPEQYNYTVLKKRMQQTIDKAKGVVEDKYVALVERGIQKLDEVIEM
                     MKQQMASGQFLHLFANATPLQQAMYMLVIAWMHLWSLSITLPKMKELVGDKKGEERAA
                     LLKDNAEAAYYSGKVLASQFYLGAEFPKYFGKIEALLSGESAVIKASEEIFTGALNE"
     gene            2698..4581
                     /locus_tag="PLV81_12880"
     CDS             2698..4581
                     /locus_tag="PLV81_12880"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOM11031.1"
                     /translation="MATGVMSLNPLVDTRDTRFVLFEVLGVDKLTQYPRFADFDKDTF
                     EDVLNLAETIAVEQVYPANMEGDKIGVKYEPDTKKVILPECYKPALQAMYDAGFIGLV
                     NDPEIGGMGMPQAIGQCCTEFFTAASVAFSMYPALTIGALNLIKNFGTQEMKDLYIPK
                     MITGQWTGTMCLTEPDAGSDVGNLKTKAIRQSDGTYKIIGQKIFISGGDYDMPENIVH
                     PVLARIEGDPPGTKGISIFIVPKYRVKPNGSLEFNDVICSGIEHKMGIKASATCTLNF
                     GDNGDCIGWLLGQERQGMKIMFQMMNEARLYVGLQGLAVSSAAYMHAIKYARNRIQGV
                     DVTQMLNPDAPKVTIIKHPDVKRMLLWMKSYVEGMRLLTYYVGYQEDLVHVLDGEAKK
                     EAQAMIEFLIPITKAGNTDNAWKVTAEAIQVYGGYGFCSDYPVEQFARDAKILSIYEG
                     TNGIQSMDLTMRKILMNPEQYNYTVFKKRVQETIDRAKGIVDDKYVAIVQKGLEKVDT
                     VVNYLKDQMAAGKFLHIFANATPLQQALSMFTLAWLHLWMLSATQPKMKEIVGDKKGD
                     DLTVLLADNSEAAYYTGKVLSSQFFLGAEFKKFFGMVDYILDGEAAVVKANEYIFTGA
                     PLE"
     gene            5042..7399
                     /locus_tag="PLV81_12885"
     CDS             5042..7399
                     /locus_tag="PLV81_12885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOM11032.1"
                     /translation="MKTHTQKQFAFLEYINQPVIVFTKQGVIVTANTSFASIVSMPLD
                     KLVGSTVGAVFADAQKIHKITQKLDHTKKVVSVTDVVLQNFEKINVSIVHIDDEHYCL
                     IAEEKFADIEKHYKLLQSILDNIPRQIVVLDKNSAIVLANKVWLQFINTVLGTNFKPA
                     QVTEKNFFDFCNQIECFDHNFQMLLHSAIEKIIHNEAETITLENTIYLAGIPHWFKFV
                     ISRYSLNGTSYCVLLFNDITEQKQFQDQIKEQRDTFQKYLDISPYMMVILNEKGTITL
                     ANKKACQILGYAYEELIGKGWFDTCVPQDMREDVRNVYFELMKGNIQPVEFYENPVVT
                     KDGSLRLIAWHNTVLNDAHGNIIGTMSSGEDITEQKMMEIELLKSTTNLKAIVKAFPD
                     MYIVADKDGIIQDYQFGEEMKLYATKDQVVGKKYYHIMPEHITELYRNALNEVFTYNK
                     IVGIEYPIDIEGETRWREARLVPMIKDRVMVVIRDVTQRKHMEIALAESEKRYRHLVN
                     RIPAAVVEMNVNGDIVFVNDYFVHLTGYTLDNLKGTHWFRSILHPHEDKRKIRQFVEL
                     IQQGDVSNFEIRIINREGHDTHITITTSNVYNAVGKLERIVCIATDITPIVMLRDKLK
                     ELAIKDELTGLYNRRGFTMLAEQQMKLSKRSGKGIALFYIDMDNMKTINDTMGHHEGD
                     KALIDVAGILRLSFRDSDIIGRLGGDEFAVLAIDCEKHFMEMMQQRLLDNAEEFNKKG
                     LRMYTISLSVGSIFIGDSSSSLDEVLSLADSLMYKNKMQKKQHRL"
     gene            7490..7726
                     /locus_tag="PLV81_12890"
     CDS             7490..7726
                     /locus_tag="PLV81_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOM11033.1"
                     /translation="MLAKKTSKNQLTLPAKIVKEFPDIDYFDITVKDNKIIMVPVVLK
                     PQTGINTIREKMKKLGINGKDIEEAITWARKKKK"
     gene            7702..8142
                     /locus_tag="PLV81_12895"
     CDS             7702..8142
                     /locus_tag="PLV81_12895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HOM11034.1"
                     /translation="MGKKKEKITQEIVVVLNTNIVVSAILFKGKVSTLMALWHNGTIK
                     PVMTKEIFDEIHAVLKYPKFMLTKHEIDYILNEELLLFFDVITDNYPNISVCKDEDDD
                     KFIRCALSAKAKYIITGDKELLQIKKYKDIEIMSCNQFLNLFKK"
     gene            complement(8162..8698)
                     /locus_tag="PLV81_12900"
     CDS             complement(8162..8698)
                     /locus_tag="PLV81_12900"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOM11035.1"
                     /translation="MYYDMIMAGFGGQGIQIISQLVAVAAINKGYEVTYLPSYGVEKR
                     GGRTNVFVVISDEEIGSPITNHPYALLVMDQIAYDTYIHILRPDGLLVANESLIDTTT
                     HRRNDIVSCCLFCNEEAVKLGNAKLANMIALGALLKNSNVLTVDDIEKVIDQVIPERL
                     AHTIPANMQAIRHGYKLA"
     gene            complement(8729..9481)
                     /locus_tag="PLV81_12905"
     CDS             complement(8729..9481)
                     /locus_tag="PLV81_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOM11036.1"
                     /translation="MQAVFTRPKTLKDTPTHFCPGCTHGIAHRIVAEVIDELGIREKT
                     IGIPGVGCCVFLYKYLDIDVLEAPHGRAPACATGMKRVLPDRIIFAYQGDGDMAAIGT
                     SEVIHAANRGENITVIYINNSVYGMTGGQMAPTTLLGQQTTTTPYGRNFRSEGYPIRM
                     AEMLATLEGVAYSARVALHKPKYVMQAKKAVEKAFMMQINEMGFSVVEMLSTCNTNWR
                     VSPTEAIDKLENEMIPYFPLGVFKERTGKDFL"
     gene            complement(9492..10535)
                     /locus_tag="PLV81_12910"
     CDS             complement(9492..10535)
                     /locus_tag="PLV81_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOM11037.1"
                     /translation="MRVLTKGNIALAEGAIQAGCRYYFGYPITPQNDIPEYLAKHLPR
                     VGGTFIPAESEIASINMILGASAAGARAMTSSSGPGISLMQEGFSYLAGSELPAVIVN
                     IMRIGPGLGGIAPTQGDYNQAVHGGGHGDYHNIVLAPASCQEMFDLTIKAFELADKYR
                     NPAMILADAMVGQFQEPCRLTPKEPVVMPEKPWALTGAKGRPAQLIKSLFLGPGEMEI
                     HNLDLKKKYDAMEQNEVMYETRHLDDADIMIIAFGTPSRIAKTAIQFLRQEGVRAGLL
                     RPITLFPFPSKAIHEYSQRIKKILVVEMNTGQMLRDVQLACHNGTRVYFYGRPGGGIP
                     YPDDIMAQVKKLL"
     gene            complement(10532..10741)
                     /locus_tag="PLV81_12915"
     CDS             complement(10532..10741)
                     /locus_tag="PLV81_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOM11038.1"
                     /translation="MAHIVIDTNRCKGCYFCVKYCPKQIITISKQYNAIGYFPAELSP
                     DKKDLCTGCKTCALMCPDTAIEVYK"
     gene            10990..11361
                     /locus_tag="PLV81_12920"
     CDS             10990..11361
                     /locus_tag="PLV81_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11039.1"
                     /translation="MDSKERIALIRKGNEFFNQGKIKEAIEIFVKTGYKDGLQRIGDY
                     YYYDKKLPLIALKFYRMSGSNKKIDEIMERMIFALGKWLGNDKVKDSPFRVKLPPLKV
                     SPKLKMLAQEILEKHKEEENK"
     gene            11559..12149
                     /locus_tag="PLV81_12925"
     CDS             11559..12149
                     /locus_tag="PLV81_12925"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOM11040.1"
                     /translation="MNVQNLDENLIAILDKLTDIKRSMLWKISEHYNLTPLQIQILQY
                     INGCSATRNITPSDIVKNLYISKATASVALKTLQQKAMIRKNVDKHDSRSYYLTLTKK
                     AENILTQIEQSKRDVIQFLENMSSNDKKAAFTVLTQLVTAMQNNGIIDYVALCINCEF
                     CKNVKPHTFQCTVTGRIFEYDGINVGCCNFLDKRAV"
     gene            12191..12544
                     /locus_tag="PLV81_12930"
     CDS             12191..12544
                     /locus_tag="PLV81_12930"
                     /inference="COORDINATES: protein motif:HMM:NF007714.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HOM11041.1"
                     /translation="MATNNKAIEHDIGVLGKFIQIYCNGKHNGQKSKVKGNGKIAQYI
                     DPLNVELCDDCKKLLLYAASKRIICPYDPKPACKDCTTHCYAEPNKSKIREVMRYSGM
                     RMIFKGSISYIKKFL"
BASE COUNT         4137 a         2113 c         2622 g         3735 t
ORIGIN      
        1 agtttgcaaa ggatgggttt cactatttgc tgtggttatt gtggtattta gcctataatc
       61 ccatacgggg aagattatgc tctgacccta cgaaatacca ctacagcagc atacgagcat
      121 atttagaaga agatgccgat gtaggagtac ccatagacca tcatgattac tttgtgcagt
      181 tggggaaaac gtttgctgag agggtagtaa agtttatgtg gtatgaggag tattaccgga
      241 aaaagtattc gtatgtggtg gattgggtgt aaaaaaaata agaaaagggg tcagagcatt
      301 attacagagc attattacaa tttactaaat atgtgcatat cagttttaag acgataaaat
      361 atattttttc gtatgtttga aataaattat acaaaaaaat atattttcat tattgacaga
      421 aaaatactgt attgtagcgt tactatgaat ttgtgtgtat gtgaataata tcttaacata
      481 cacctgaatg tgtaatattt taacatttgt tataatttaa aagcaggagg ttttttatgg
      541 cgttaaatcc actggttgat tcaagggatg tgcgctttgt cctctatgaa atgatggatg
      601 tattgcagct gacaaagctt ccaaagtact cttcatttga caaggatatg tttgaggaca
      661 tgctcaattt agcagaaact atggctgttg agcagattta tcctacattt gctgaagggg
      721 acaaagaagg ttgccattat gacggagcaa ccaaaaaggt atctattccc aaatgttatc
      781 atgcagcact tgatgcattt tatgaagccg gttttggtgg aatggatgta tcagatgagt
      841 ggggtggtat gggtatacca caggtaattt cctgggcatg taatgaattt ttctgtgctg
      901 ctaactaccc attattgatg tatcctggat taactcatgg tgctgcagag cttattgagg
      961 catttggcac cgaagagcag aaacatatgt accttgcaaa tatgtattca ggaaaatggg
     1021 gcggaacaat gtgtttaact gagcctgatg caggttctga tgttggagca ttgaagacaa
     1081 aagcagtccg acagcctgat ggtacattca agatctatgg gcagaaaata tttatttctt
     1141 ctggcgacaa tgactattat gaaaacatga ttcatccggt attagcacga attgaaggag
     1201 acccacccgg aacaaaagga atatctattt ttatagttcc caaatacaga gtaaaccctg
     1261 atggatcatt gggtgaattc aatgacgtaa catgtgctgg tattgagcac aagatgggta
     1321 tcaaaggttc agcaacctgt acattaagct ttggtgataa taataactgt attggctggc
     1381 tacttggaca ggaacgccag ggtatgaaga tcatgttcaa gatgatgaac tatgctcgta
     1441 tgggtgttgg tcttcaggct catggatgtg cttcagcagc atacatgcat gcagttacat
     1501 attgtaagaa ccgtgttcag ggtgtacatg taacccagat gctaaatcct gaagcaaaag
     1561 gtgtaaccat cattaaccat ccagatgtca agagaatgct tctgtggatg aagtcatatc
     1621 tggaaggcca gagagctatg atttactatc tgtacctcaa ttatgaactg gcacagcttt
     1681 tagaaggcga agcagcaaaa gaagcacagg cactggttga gataattact cctatagaaa
     1741 aagctggttg ttcagatatg tcagtattaa tcacctcaga agctatgcaa tgctatggtg
     1801 gatatggatt tgttgctgac tatcccgttg agcagatgat gagaaattca aagataactg
     1861 caatatatga aggaacaaac ggcatccagt caatggacct taccatgcgt aagattttga
     1921 tgaaccctga gcagtacaat tatacagtgc ttaagaaacg catgcagcag actatcgata
     1981 aggcaaaggg tgttgttgaa gataaatatg tagcattagt tgagcgtgga attcagaagc
     2041 tggatgaagt tattgaaatg atgaaacagc agatggcttc aggacagttc ctccacctgt
     2101 ttgccaatgc aacaccatta cagcaggcca tgtatatgct ggttatagca tggatgcatc
     2161 tgtggagctt gagtataaca ttgccaaaga tgaaagagct tgttggcgac aagaaaggtg
     2221 aagaaagagc agcattactt aaagataatg ctgaagctgc atactacagc ggaaaggtat
     2281 tggcttcaca gttctattta ggtgctgaat ttccaaaata ctttggcaaa attgaagcgc
     2341 tccttagtgg tgaatctgct gttattaagg caagtgagga aatatttacc ggagcgttaa
     2401 acgaataaga gatgttatat acagtcttac ccaaaaaagg caggtttttt ccctgccttt
     2461 ttttatataa tttattaatg aaatgagtca ctgctatgac aggggctcat tgtttgatag
     2521 ttgttttcat taatttgtta ttataaaaaa aacaaaattt aacatcaaag aacatcattt
     2581 atatgttaac aaattattga caaaataaat aaaaaaaata ttgtgaaatc aggaattatg
     2641 gtatttaaca taataccacc aaatatatat taaaattgta atacaggagg tctttttatg
     2701 gcaactggag taatgtcgct aaacccactg gtggatacac gggatacgcg atttgttttg
     2761 tttgaagtat taggtgttga taaattaact caatacccgc gatttgctga ttttgataaa
     2821 gatacatttg aagatgtatt gaaccttgca gaaacaattg ctgtggagca ggtatatccg
     2881 gcaaatatgg agggggataa aattggtgtt aagtatgaac ctgatacaaa aaaagtaata
     2941 ttacctgaat gttataaacc agcattacag gcaatgtatg atgcgggttt tattggattg
     3001 gtaaatgatc ctgaaattgg tggtatggga atgccacagg caattggcca atgttgtaca
     3061 gagtttttta ccgctgcaag tgtagccttt tcaatgtatc cagcattaac cataggtgca
     3121 cttaacttga taaaaaactt tggtactcag gaaatgaaag acctgtatat accaaagatg
     3181 attacagggc agtggacggg aaccatgtgt ttgactgagc ctgatgcagg ttcagatgtt
     3241 ggtaatctta aaacaaaagc tatcaggcag agtgatggta cctataaaat tattggccag
     3301 aaaatattta tcagtggtgg cgattatgac atgcccgaaa atatagttca tcctgtactt
     3361 gctcgtattg aaggagatcc acctggaaca aaaggtatat ctatttttat tgtgcccaaa
     3421 taccgtgtaa aaccaaatgg ctcactggaa tttaatgatg ttatatgttc aggaatagaa
     3481 cataaaatgg gtatcaaagc ttcagctacc tgtacattaa attttggtga caatggcgac
     3541 tgtataggct ggttgctggg gcaggaacga cagggtatga agatcatgtt ccagatgatg
     3601 aatgaagcgc gtctatatgt tggtttgcag gggctggctg tttcaagtgc tgcatatatg
     3661 catgcaataa aatatgcacg caaccgaatt caaggtgtgg atgtaacaca aatgcttaac
     3721 cctgatgcac caaaagtaac aataatcaaa catcctgacg taaaacggat gttgttgtgg
     3781 atgaagtcgt atgttgaagg tatgaggctt ttaacatact atgttggcta tcaggaagac
     3841 cttgtccatg tacttgatgg cgaagccaaa aaagaagctc aggcaatgat tgaattcttg
     3901 atcccaataa caaaagctgg taatactgat aatgcatgga aagtaacagc cgaggccatt
     3961 caggtatatg gtggttatgg attctgttct gattatcctg ttgagcagtt tgcacgcgat
     4021 gcaaagatac tttcaatata tgaaggtacc aacggtattc agtcaatgga cttaacaatg
     4081 cgtaaaatac ttatgaaccc tgaacagtat aattacactg tattcaaaaa acgggtacag
     4141 gaaactattg acagggctaa gggtattgta gatgataaat atgtcgctat agtccagaag
     4201 ggtcttgaaa aagttgatac agtggtcaac tatctaaaag accagatggc tgctggcaaa
     4261 ttcttgcata tctttgccaa tgcaacacca ttgcagcagg cactgtccat gtttacatta
     4321 gcgtggctgc atttatggat gctttcggct acacagccaa agatgaagga aatagtgggc
     4381 gacaagaaag gtgatgatct cacagtatta ctggcagata atagtgaagc tgcgtattat
     4441 actggaaaag tattgtcatc acagttcttc cttggtgctg agttcaagaa attctttggt
     4501 atggttgact acatccttga tggagaagca gcagtagtta aagccaatga atatattttt
     4561 actggtgcac cattagaata aaattaaata ttatagcaac aaagaggggc tgtcccaaaa
     4621 gaggtcgttg agtaaatttt gcaaacatta taacgtaata ttggctcatt tcgatacaat
     4681 tttgccgcaa aatctggcaa aattactcaa tgagcgttgc aaaatttgta tcgaaacgac
     4741 ttttgggacg ccccttttat ttatatgaag attggggtca gagcatacta tcgagcatgc
     4801 aaattttatg cattaaattt ttagcagttg ccagcaatga aatattctgc aatgttgtat
     4861 ctatcaccac tttttcttga aatatccttt tgtaattgtt acaattaaat aacaactata
     4921 ataaaaaatc agtataattt tatgaactat cgccaatgca tgtacatttt caaaaaattc
     4981 atttgcatta taacatattt aatatactgt atctaacaat gtttgccagt gctggatgta
     5041 tatgaaaact catacacaaa aacaatttgc cttccttgag tatatcaacc aacctgttat
     5101 tgtttttaca aaacaggggg tgatagttac cgccaataca tcctttgcca gcattgtatc
     5161 catgcctctg gataagctgg ttggctccac ggttggcgca gtctttgctg atgcacagaa
     5221 aatacataaa ataacacaga agcttgacca cactaaaaag gtggtatcag ttacagatgt
     5281 tgtactacaa aacttcgaga aaataaatgt ttccattgtg catatagatg atgaacatta
     5341 ctgccttatt gctgaagaaa aatttgcaga tattgaaaaa cattataaat tgttgcaatc
     5401 gattcttgat aatataccac gacagatagt tgttctggac aaaaacagtg ctattgttct
     5461 tgccaataaa gtttggctgc aatttataaa taccgtgctt gggaccaatt ttaaacctgc
     5521 acaggttaca gaaaaaaatt tctttgattt ttgcaatcag atagaatgtt ttgaccataa
     5581 cttccaaatg ctattgcatt ctgctattga aaaaattatt cacaatgaag ctgaaactat
     5641 aacgcttgaa aatactattt accttgctgg tattccgcac tggtttaagt ttgtcatttc
     5701 acggtattcc ttaaatggca catcatattg tgtactgttg tttaatgata taaccgaaca
     5761 gaagcagttc caggatcaga taaaagaaca gcgtgatacc ttccagaagt atttagatat
     5821 atccccctat atgatggtaa tactcaatga aaagggaacc attaccttgg caaataaaaa
     5881 agcatgccaa atattaggat atgcgtatga agagctcatt ggcaaaggct ggtttgatac
     5941 gtgtgtgcct caggatatgc gtgaagatgt gcgtaacgtt tattttgaat tgatgaaagg
     6001 caatatacag ccagtagaat tttatgaaaa tccagttgtg acaaaagatg ggagtttgcg
     6061 cttgattgcc tggcataaca ctgtattaaa cgatgctcat ggcaacataa ttggcactat
     6121 gagttcgggt gaagatatca ccgaacaaaa aatgatggag atagaacttt taaaaagtac
     6181 caccaattta aaagcaatag tcaaagcgtt ccccgatatg tatattgttg ctgataagga
     6241 tggcattata caggattacc agtttggtga ggaaatgaag ttatatgcta ccaaggatca
     6301 ggtcgtaggt aaaaaatatt atcatataat gcctgaacat ataaccgagt tatatcgcaa
     6361 tgcattaaat gaagtattta catataataa gattgttggt atagaatatc ccattgacat
     6421 tgaaggggaa acacgctggc gcgaggcaag attagtaccc atgataaaag acagggtcat
     6481 ggtggtgata cgggatgtta cgcagcgtaa acacatggaa attgccttag ctgaatctga
     6541 aaaacgatac cgccatctgg ttaatcgaat tcctgcagcg gtggttgaaa tgaatgtcaa
     6601 cggtgatatt gtatttgtaa atgactattt tgttcatttg acaggatata cactggataa
     6661 ccttaaaggt actcactggt ttagaagcat cctccatcct catgaggata aaagaaaaat
     6721 acgccagttt gttgaactca tacagcaggg tgatgttagc aactttgaaa tacgtataat
     6781 aaatagagaa ggccatgata ctcatataac cataactaca tcaaatgtat ataatgcagt
     6841 gggaaaatta gaacgtatag tgtgtattgc cacagatatt acgcccattg tcatgttacg
     6901 ggataagctt aaagaactgg caattaaaga tgaacttaca ggcctgtata accgtcgtgg
     6961 atttaccatg ttagctgagc agcaaatgaa attaagcaaa cgcagcggca agggcatagc
     7021 acttttctat attgatatgg acaatatgaa gacaatcaat gataccatgg gacaccacga
     7081 aggcgataaa gcactgattg atgtggctgg aattttgcgt ttatcgttca gggattcaga
     7141 tatcattgga aggttaggtg gtgatgaatt tgcagttctt gcaattgact gtgaaaagca
     7201 ttttatggaa atgatgcagc aacgattgct tgataatgct gaagaattta ataagaaagg
     7261 tcttcgcatg tatactattt cactcagtgt gggaagtata tttattggcg acagttcttc
     7321 atctttagat gaggtattat cgttggctga ttctttaatg tataaaaata aaatgcagaa
     7381 aaaacagcac cgcttgtaaa gccaaaaaaa tttatagttt tttataaaaa atatttattg
     7441 ataaaatatt atgataaggt attatatcct tactgtaagg aagtaaggaa tgcttgcaaa
     7501 aaaaacatca aaaaatcagt taacattacc agctaaaata gtaaaagaat tccctgatat
     7561 tgattatttc gatataaccg tcaaagataa taaaataata atggttcctg ttgttctgaa
     7621 acctcagaca ggcattaata ctataagaga aaaaatgaag aagttaggta ttaatgggaa
     7681 agatattgag gaagctataa cgtgggcaag aaaaaagaaa aaataaccca ggagattgtt
     7741 gtagttctta atacaaatat tgtagtatct gcaattcttt ttaagggcaa agtttcaacg
     7801 ttaatggcat tatggcataa tggcactata aagcctgtta tgacgaaaga aatttttgat
     7861 gaaatacatg ctgttttgaa gtatccaaaa tttatgctta caaagcatga aatagattat
     7921 attcttaatg aagaattatt acttttcttt gatgtgatta cagataatta tcccaatatt
     7981 tcggtttgta aagatgaaga tgatgataaa tttatacgtt gtgctttatc agctaaggca
     8041 aaatatatta ttaccggtga taaagaattg ttgcagataa aaaaatataa ggatatagaa
     8101 ataatgtcat gtaaccaatt tttaaattta tttaaaaagt aatgtatatt gtatgattgt
     8161 tttaagctaa tttatatcca tggcgtattg cctgcatgtt tgcaggaatg gtatgtgcca
     8221 ggcgttcagg gattacctgg tcaattacct tttctatgtc atctaccgta agcacattac
     8281 tgttttttaa taatgcaccc aatgcaatca tattagctaa tttggcattg cccagtttaa
     8341 cagcctcttc attgcaaaac aaacagcacg atacaatatc attgcgccga tgtgtggttg
     8401 tatcaatgag gctttcgttt gcaacaagca aaccgtcagg cctcaatata tgaatatagg
     8461 tatcatacgc aatctgatcc ataacaagta atgcatatgg atgattggta atgggtgatc
     8521 ctatctcttc atcagaaatt accacaaaaa cattggtgcg tccgccacgt ttttcaacgc
     8581 catacgatgg aaggtatgta acctcgtagc ctttattgat ggcagcaacg gcaacaagct
     8641 ggcttataat ctgaataccc tgtccgccaa aacctgccat aatcatgtcg taatacattg
     8701 aataactcct tcaacagtaa ctatcggttt acagaaaatc ttttcccgta cgttctttga
     8761 agacgcccag cggaaaatat ggtatcattt cattttcaag tttatcaatt gcctctgttg
     8821 gcgaaacgcg ccagttggtg ttgcaggttg aaagcatctc aacaacggaa aaccccattt
     8881 catttatctg catcatgaaa gctttttcca cagctttttt tgcctgcatg acatattttg
     8941 gcttgtgcaa tgcgacacgg gccgaatatg caacaccttc cagcgttgca agcatttcag
     9001 ccatacgaat gggatagcct tcactgcgga aatttcgtcc atacggtgtt gttgttgttt
     9061 gctggccaag caacgttgtt ggtgccatct ggccgcctgt cataccatac actgaattgt
     9121 taatgtatat gactgtgatg ttttcgccac ggtttgctgc atggattact tcagaggttc
     9181 caatggctgc catatcgcca tcaccctgat aggcaaagat tatacgatca ggaagaactc
     9241 gtttcatgcc ggtagcgcat gctggtgctc ttccatgtgg tgcttcaagc acatcaatat
     9301 ccagatattt atataaaaat acacagcagc cgacgcctgg aatgccaata gttttttcac
     9361 gtataccaag ctcatcaatg acctccgcaa caatgcggtg tgcaatacca tgagtgcatc
     9421 cagggcaaaa gtgtgttgga gtgtctttta acgtttttgg tcgtgtaaat actgcctgca
     9481 tggtagcctc actaaagtag tttttttacc tgtgccataa tatcgtcagg gtaggggata
     9541 ccgccacctg gcctgccgta aaaatatact ctggtgccat tgtggcatgc taactgtaca
     9601 tcacgcagca tctggcctgt gttcatttct acaaccagta tctttttaat gcgctgagaa
     9661 tattcatgta ttgcttttga tggaaatggg aataacgtga ttggtctaag caaacctgct
     9721 ctgacccctt cctggcgtaa aaattgtatg gcagtttttg caatgcgtga aggagtgcca
     9781 aaggcgataa tcatgatatc agcatcatcc agatgacggg tttcgtacat aacttcgttt
     9841 tgttccatag catcatattt tttctttaaa tccaggttgt gtatttccat ctcacctggg
     9901 ccaaggaata atgatttgat aagctgtgca ggcctgccct ttgcaccggt aagggcccat
     9961 ggtttttcag gcattaccac cggctcttta ggtgttaaac ggcatggttc ctgaaactgc
    10021 ccaaccatgg cgtcagccag tatcatggca ggatttcgat atttatcagc aagttcaaat
    10081 gcttttatgg ttaagtcaaa catctcctga catgacgcgg gtgccagcac tatgttgtgg
    10141 tagtcgccat gaccgccacc atgcactgcc tggttataat cgccctgtgt tggagctata
    10201 ccaccaagac ccggcccaat acgcatgata ttcacaataa ctgctggaag ctctgacccc
    10261 gctaaatacg aaaaaccttc ctgcatcagt gaaatgccag gtccagatga tgacgtcatt
    10321 gctctggcac cagcggcgga tgcaccaagt atcatgttga ttgaagcaat ttcgctttca
    10381 gctggaataa aggtcccacc aacacggggc aaatgtttag ctaagtattc gggaatatcg
    10441 ttttgaggcg ttatgggata gccaaaataa taacggcatc ctgcctgtat tgcaccttca
    10501 gcaagtgcaa tatttccttt tgtcaggact ctcatttgta cacctctata gcagtatccg
    10561 ggcacatcag tgcacaggtc ttgcaaccgg tgcacaggtc ttttttatcg ggcgatagtt
    10621 cagcggggaa atagccaatt gcgttatatt gttttgaaat ggtgattatc tgcttggggc
    10681 agtacttaac acagaagtaa catcctttac aacggttggt atcaatcact atatgcgcca
    10741 tgggctcatc tcttcagtca aattgtattg tacggtaact atcgccaaac taacagttta
    10801 cgttaactgt tagagaaact acataaataa tacttgcaaa cagtacgata ataataaaaa
    10861 gtactagtaa acaaatctat cgctaagcat actatatgtc aataaaaagt agataggtta
    10921 tacaaaattg tgttgtacat aaatttgtaa tgatagttat gtgcgatagt tactgtgaaa
    10981 aggtgattga tggatagtaa agaacgaata gccttaatac gcaaaggaaa tgaatttttt
    11041 aaccagggca aaatcaagga agccattgaa atttttgtta aaaccggtta taaagatgga
    11101 ctgcaacgca taggcgatta ctattactac gataaaaagc ttccactgat tgcattgaaa
    11161 ttttacagga tgagtggaag caataaaaag atagatgaaa taatggaacg gatgatattt
    11221 gctcttggca aatggctggg caatgataaa gttaaagata gcccattcag ggttaaactc
    11281 cctcccttaa aagtatcccc caaattaaaa atgctggctc aggaaattct ggaaaaacat
    11341 aaagaagaag aaaataaata aaaaactcag tgaaaatttt tataactgct ttttgtagtt
    11401 aggtagtgaa tgagtatcct tgctgaaacg gtcaaagtta aaacagcatg tattgcctta
    11461 aaaaattatg aataaacact gaaaaaaatt agaaaaaaat ttgacatttt ttatatgcag
    11521 attatagtta gtactactaa ctatatgaag ggttatatat gaatgtacaa aaccttgatg
    11581 aaaaccttat agctatactt gataaattaa ctgatatcaa acgttcaatg ctgtggaaaa
    11641 tatctgaaca ttataatctt acgccattgc agattcagat attgcaatac attaacgggt
    11701 gttctgcaac cagaaacatt acaccatctg atatcgtgaa gaatctatac atcagtaaag
    11761 ctactgcaag tgttgcactg aaaacgttgc agcaaaaggc catgatacgc aaaaatgtag
    11821 ataaacatga tagccgcagt tactatctca cattaacaaa aaaggcagaa aatattttaa
    11881 cacagataga gcaatcaaaa agggatgtta tacagttttt agagaatatg tcatccaatg
    11941 ataaaaaggc tgcattcaca gtgttaactc aactggttac agcaatgcag aataatggca
    12001 tcattgatta cgtggcctta tgtataaatt gtgaattttg caaaaacgta aaaccacaca
    12061 catttcagtg cacagtaaca ggacggattt ttgaatatga tggtattaat gttggatgct
    12121 gtaattttct ggataaacgg gcagtgtaaa ttttgtgtgt tgggaattat tttataatca
    12181 ggagtaatgc atggctacaa acaacaaagc aattgaacat gatattggcg tattgggaaa
    12241 atttattcag atatattgta acggtaagca taatggtcag aagtcaaaag taaagggcaa
    12301 tggaaaaatt gctcagtata ttgacccact taatgtagag ttgtgtgacg attgtaaaaa
    12361 attattgctt tatgctgcaa gcaagcgtat tatatgccca tacgatccca agcctgcctg
    12421 caaagattgt accacccatt gctacgccga acccaacaaa tctaaaattc gtgaggtcat
    12481 gcggtattcc ggaatgcgga tgatcttcaa gggaagtatt tcatatatta agaaattttt
    12541 gtaatttata atggaggatt acatgagcga atttttaaca gatatgggta aaaagaagga
    12601 gcagctt
//
LOCUS       DAORYL010000088        12459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_3958_length_12459_cov_11.980813, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000088 DAORYL010000000
VERSION     DAORYL010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12459
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3958_length_12459_cov_11.980813"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(517..1266)
                     /locus_tag="PLV81_12935"
     CDS             complement(517..1266)
                     /locus_tag="PLV81_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11042.1"
                     /translation="MGMHLSADVYDIFEDVFKGKEKAKKVMSALEEVIVTTVHDSWYK
                     TKEELKMEVFSHYATKQDLEELRKELSGKFDVLYEKTEKDKAELLGIINQNKEELLGI
                     MKQDKAELTGKIDGLYEKTEKDKAELLGIINQNKEELLGMMKQDKAELLGKIDALYQK
                     TEKDKVELLGIISQNKEELLGKIDALNEKTEKDKAQLTEKIERVRAELLIKLEKLDKK
                     FSIYFAVLLFAIIFLNQNALEFIAKVVGLVK"
     gene            1720..1989
                     /locus_tag="PLV81_12940"
     CDS             1720..1989
                     /locus_tag="PLV81_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOM11043.1"
                     /translation="MIQENCWTFPKEIPLEEVPQYSSRFEKIEKPASLQFDLTQTEWI
                     HSSFIGFLIYAKHSISSHGGKLYIIPSPSVQRILHLMNLNDYLLH"
     gene            2156..2494
                     /locus_tag="PLV81_12945"
     CDS             2156..2494
                     /locus_tag="PLV81_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11044.1"
                     /translation="MNNNTQNIITQLKEELIPLIKTVVQDEVKSNIEEFMRQSELRAK
                     ELSLIERIIRVEEELKSLKEVEKARFEAIESRFESMEKRFTSLQWFIGIGFAIISILI
                     AVFGLFIGKV"
     gene            2755..3045
                     /locus_tag="PLV81_12950"
     CDS             2755..3045
                     /locus_tag="PLV81_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOM11045.1"
                     /translation="MIFSEEQVGFTSELLKSIAHPIRLKVLCFLMDGEKTVGEIEKEF
                     GSTISNISQHLTILRKMNIIHRRKEANFMYYSIKDERILKLISTLKNLYCAQ"
     gene            3035..4630
                     /locus_tag="PLV81_12955"
     CDS             3035..4630
                     /locus_tag="PLV81_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11046.1"
                     /translation="MRNKCILLLPVLILFLYSDVFSLPKDITIQGTVIYNQDYLVRAA
                     DIDTIPASKFTFGTIAAAITKFYHDNGYTLARVLLVRETLASIAVFVDEGRLNRIVFK
                     NLNSLDTLRIQYDFSLPNRIYHTQTVDKQLEKLKKKYAIKDFIVTLQESKDYSSALFQ
                     IDRLSKPFTDGKLPLVPTIPAVPDPHYDLIITIVAFSPEESGGFTYGLDTSFSLGLIP
                     YVRYTHNDLIQRGDSFRIGFKTGFMYGLDGNFTSLPYNTFNQIKSTYNFRSHFDGLFI
                     PRVSSEVYNSKAGRKDLGLDQYRYVRVNVLAEPGFTPIEKLKVYPGYGVEKVFILQAV
                     KNPDTAVTQPNIAKDTQVWHFIGIKANIDLIPFSLKHTTGRKITLEAYRYQNATHFYK
                     VGIDTIVKFEFFNFDLFVFQGDYTYLNDPVPFYYEEPVSTRTFKGFMGKDYHTTHIAR
                     ASFEYEISIHRDFIYTGVYNDFTAFKGKYLLHGNQIGIAYGLSFHYVFFEQFEFALWW
                     GKDYLPSTDQSGYNLKFTLAKKW"
     gene            complement(4881..5012)
                     /locus_tag="PLV81_12960"
     CDS             complement(4881..5012)
                     /locus_tag="PLV81_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11047.1"
                     /translation="MIKDNSSLCVAIAHTIIHTTNTKKEYPVIIDRGGMQVKVNEGG"
     gene            complement(5076..5570)
                     /locus_tag="PLV81_12965"
     CDS             complement(5076..5570)
                     /locus_tag="PLV81_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="HOM11048.1"
                     /translation="MGFAMRVYEAFKDDEVKAKVLAEFIEKVEEAINNNQVATRQDLH
                     VQELKLTKEIEQTRLEIKEAELKLTKEIEQVRADMKETELKLTKEIEQVRVDLTKDIE
                     KMRLEIKEIELKLTKEIERVRAELKIEIQQTKVSLLKWLIGLLLTQTITIIGVIIGLF
                     QVIK"
     gene            complement(6087..6893)
                     /locus_tag="PLV81_12970"
     CDS             complement(6087..6893)
                     /locus_tag="PLV81_12970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOM11049.1"
                     /translation="MEFATLLHDTVFCALDCETTGLNPIIDRIVSIGIIRFTLDRVID
                     TFYTLVNPLKPIPPHVTRLHGITADMVQKAPVITELFDTIEQYTSNTYCVIHNPRFDI
                     SFIDSAYHQHDRNTPDLKSFDTVHLARKTFKKLPSYNLGNLCKFFGISINAHHALSDA
                     QGSMEIFKKVIDKLNAHNWCIRDLERYHGGIISAYSLGIKSTHIPADSRIPLGYRITI
                     RYKDAQGNIVERSILPQRYFIMGKNNYIQAYCYLRNEIRYFNTARIVAVY"
     gene            complement(6899..7819)
                     /gene="murB"
                     /locus_tag="PLV81_12975"
     CDS             complement(6899..7819)
                     /gene="murB"
                     /locus_tag="PLV81_12975"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOM11050.1"
                     /translation="MGQTCSHDVIKELSQYGRLYVDVPMSQFTTFKVGGPADMLIEPC
                     SNKTIPYIVTIAKQTNTPVTIIGGGSNLVVGDRGIRGLVVRMGSSQEDEGSIAFDGTN
                     VVVDAKVTKKRFVEEAIEHGLAGMEFIAGIPGCIGGGVIMNAGTTMGTFSDIITDITC
                     YTADGTLLTVANTSLFSYRKSMLPEGAIVISARCTLNIADNPEDVKNTVKEILRERKN
                     KHPLDYPSAGSVFKNPDGHASWKLINDAGLRGYSIGGAKVSELHTNFIVNTGKATAMD
                     IKHLVEFIQETVHKLYDINLEPEIRFIGEF"
     assembly_gap    8290..8389
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8424..9095)
                     /locus_tag="PLV81_12980"
     CDS             complement(8424..9095)
                     /locus_tag="PLV81_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11051.1"
                     /translation="MIVDKTSSDAYFKTMEEMKKTKIKSYWDLLSVLKEHPDWLEELR
                     KLILTEELLALPKKFDEFVKRYESFIENEFKPLKATVEKQGNDVGSLKGDNFERKVKE
                     KAPSYFGKLILKCRLIDTMELANSIDDAILNKKATITDDERDDALKVDVVVTGFLRHN
                     KEKKVFLAAEVSIVVDKTDVERAARRTKIIEKCLGIPGIPVVIGDEYTDGAKKAAEEL
                     QVIMI"
     gene            complement(9131..9868)
                     /locus_tag="PLV81_12985"
     CDS             complement(9131..9868)
                     /locus_tag="PLV81_12985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOM11052.1"
                     /translation="MALLQVKSVSVQYDERKVLDDVSFEVQESTITALTGKSGSGKTT
                     LLGVLSGLLQPNEGTVLFMGDNIFTWSDLKRSRFRNRDIGFVFQFFNLFSDISAYDNI
                     AFPAILNRETRNLHARIMELAEYLQITDILDRNPTTLSGGERQRVAIARAIINEPKLI
                     LADEPTGNLDTATANDILKLFARLRDDKGISIIIATHDERIVKASNVQYHLEGGKILE
                     NGVRKEEHKIVKKKPITRPAAKIKKKK"
     gene            complement(9878..11161)
                     /locus_tag="PLV81_12990"
     CDS             complement(9878..11161)
                     /locus_tag="PLV81_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOM11053.1"
                     /translation="MVQYIIRNAKLIIEEIVKNIWKALLSSFGIILLIAFLVLYLSLR
                     DSVTNFVEKRLFGSLDINEIIVEPKAKTGEATLSYAINSIPVEKVKAIRKMNTVASVY
                     TVTCINAATKIKIELLGKSRAPYVPICGISQQFIKDKVPGYKSFRYYTNEPVPVIAPQ
                     VALDMFNNFAAINNLPLFNEDTLKGFPLTLIINTPKGAETRKRFEVPAVVHSFSTLFN
                     LTGIVVPDTFVNKIAARYAAETKSAQPILYIRLYVKVKDVKKLPEVTKQIKSMGLAVT
                     SLDEVASKTNQAMSIIDGFSIFVGFFLLVLTVISIFNSYLIIIYNRSYSFSLRRILGV
                     SKFRIIMTFMVEAAFIGAVYGFIGYLAGAYLIKAAGAKLSSLVPALSGIGLEPVGMNV
                     CAGLVLFSIAISSLSALIPAIFASNMNLFKALSKQ"
     gene            11327..12103
                     /locus_tag="PLV81_12995"
     CDS             11327..12103
                     /locus_tag="PLV81_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11054.1"
                     /translation="MSNKKILIYLLFIIPFLCCATYTPVEKIYEDNKIVKIVIRDKKG
                     VTGWYIINYDTNNRITGLNKYTPYKNTPITKIQLQYKGKLLTSYTIASTSPESDTVLY
                     QSTHIFNYSYANDIRRVDILFNKPASIKQAGTTFIRIQYSYTDKLVTGLYITGDASSF
                     KISCTISYRKNTIDAITIDEKRYNTATKKFEQGLKGTLYIDKTPEYFIDEATGNKIKN
                     KDKLTSIYAKTYIQDALNKLTLTNGNEQIIDYFLLSDSSL"
BASE COUNT         3793 a         2452 c         1967 g         4147 t
ORIGIN      
        1 cttatattcc cagcccccta ttcaagggca ttatgggcat gtgtatttga gcatcctgac
       61 aacatatcgt gaattgtgga aaaggattca atgatgtaca accccctctt ctttgttttt
      121 gctatgatac atgaaatttt tgtttcttac tctcttactc tttttgagat gcgatgcatc
      181 gcatctctac cctttttgaa atgcgatgca ccgcatctct actttttgaa atgcgatgaa
      241 ctgcatctct acacgtttta ttaatacatt ttccaaaaaa tatataaata caccatcatt
      301 tatgtcaacc attttttttg cctgattgca ggctttttta tataaaactt gtaacattat
      361 aattgtgcga cgtaatgcat tacgtctata actatcgccc cataacaatt tcacattaat
      421 ttgcttaaat acctacattc acctgtatta tttgtcaaca aaaatatata ccaaccggtc
      481 ttttttttat gcaaccatcc actgttctta tatcctctat tttaccagtc ccacaacttt
      541 tgcaataaac tccagtgcat tttgatttaa gaaaattatt gcaaacaaca ataccgcaaa
      601 atatatactg aattttttat ccaatttttc taattttatc agtaactctg ctcttacacg
      661 ctcaatcttt tcagtaagct gagccttgtc tttttcagtt ttttcattga gtgcatcaat
      721 tttccctaaa agttcttctt tattctggct tattatgcct aaaagctcga ccttgtcttt
      781 ttctgtcttt tgatataatg catcaatttt tcccaatagc tcggctttat cctgcttcat
      841 catgcccaaa agttcttctt tattctgatt aatgatcccc aaaagctcag ccttatcttt
      901 ttccgtcttt tcatataacc catcaatttt accggtaagc tcagctttgt cctgtttcat
      961 tatccccaaa agttcttctt tgttctgatt gataatgcct aaaagctcag ccttatcttt
     1021 ttcagtcttt tcatataata catcaaattt ccctgaaagc tctttgcgta gctcttccag
     1081 atcctgcttc gtggcatagt gactgaacac ctccattttg agctcttctt ttgttttata
     1141 ccagctgtca tgtactgtag tcactatcac ctcttccagt gcactcatta ctttttttgc
     1201 cttttctttg cccttaaaaa catcctcaaa aatgtcgtaa acgtcagccg aaagatgcat
     1261 tcccattttt tatctccttc catttaaaat tgtaatacta tcgctataaa atgtcaacac
     1321 aattgtagat acgtaccaca attggtgttt gtatactaat acgcgtgaga ataaaaaaat
     1381 ttaaaaaaaa ttgctgaata cctgctcgtt ttacgttttt ttatgaaaaa aataaaattt
     1441 tttcttgact ttattgtatt atgtcttata tataccctaa ttttcttgac agatttttta
     1501 ttgtgttttt tttatcatca tcattttgat taatatcaat tttgctaaat agatttttca
     1561 aggtgagtta tctccccttc tgaaagaggg ctgagtataa attataaaag ttttttatca
     1621 ttgctttccg tgcatataaa aatatctaaa ttttaatgaa ataaaggacg aatatatata
     1681 cataacggta cgtgtgtttg tatggatagg aggaagctaa tgattcagga aaactgctgg
     1741 acgtttccta aggaaatccc gctggaagaa gtgccccaat actcaagcag gtttgaaaaa
     1801 attgaaaagc ctgcatctct acaatttgat cttacccaga cggagtggat ccattcttca
     1861 tttattggtt ttttaattta cgcaaaacat tccatatcct cccatggagg aaaactgtat
     1921 attattccct caccatccgt gcaacgaata ctacatctta tgaatctgaa tgattatttg
     1981 ttgcattagg gtatcattcc acatgcatct atgaccagcg ttccctcatg ggaatgtagt
     2041 gctatttggt atattacaca aaaaatacct tcatgttgaa gaatcaaaat actgttgaca
     2101 atttatctgc gtctgtttat ctttgaaaga aagaaatata tttcttgagg tatccatgaa
     2161 taacaataca caaaatataa taacccagct caaagaggaa cttatacctc ttataaaaac
     2221 tgtagtgcag gatgaagtta aatccaacat tgaagaattt atgcgccaga gcgagttacg
     2281 agctaaagaa ttaagtctta ttgaacgcat tatacgtgtt gaagaagaat taaaatcatt
     2341 gaaagaagtt gaaaaagctc gttttgaagc aattgaaagt agatttgaat ctatggaaaa
     2401 acgttttaca tcattacaat ggtttatagg tattggtttt gccattatta gcattttgat
     2461 tgccgtgttt ggtctattta ttggaaaggt ataaaaaata attaatcaag ctaccgtaat
     2521 ggatttcata cccattcctt gctaacgata gagacctaag ataaagtaaa aaaatattga
     2581 caacatcacc gatattgcta ccttgttatt aaacagtaaa ataattgttt aattaaaatc
     2641 ttaattactt gttgttttaa aacttttttt acatttgcat tttttctttt gtatagcact
     2701 atataatgag ttaacaggat tgtattatac agcacagagg gggactttat atatatgatc
     2761 ttttctgaag aacaggtggg gtttaccagc gaactactaa aatccattgc ccatcccata
     2821 cggcttaaag tattgtgttt tttaatggat ggtgaaaaaa cggtaggcga aattgaaaag
     2881 gaatttggct ccaccatatc aaatatatcc cagcacctta ccattttaag aaaaatgaat
     2941 attatacaca gacgcaaaga agccaatttt atgtattact ctattaaaga tgaacgcata
     3001 ttaaagctta tctccacatt aaaaaatttg tactgtgcgc aataaatgca ttttactttt
     3061 accagtattg attctgttcc tgtattcgga tgtttttagc cttcctaaag acattaccat
     3121 acagggcact gttatctaca atcaggacta cctggtccgt gctgccgaca ttgataccat
     3181 acctgccagt aagtttactt ttggaactat cgccgccgca ataactaaat tttatcatga
     3241 caatgggtat acgctggcac gtgttcttct ggtgcgcgaa acattagctt ccattgccgt
     3301 atttgttgat gagggacgct taaatcggat tgtgtttaaa aatttaaaca gccttgacac
     3361 gctgcgtatt cagtatgact ttagcctgcc aaaccgcatt taccatacac aaaccgttga
     3421 taagcaatta gaaaagctca agaaaaaata tgctattaaa gactttattg tgacattaca
     3481 ggaatcaaaa gattattcca gtgccctttt tcagatagac aggttgagca agcctttcac
     3541 tgacggcaaa ctgccgctgg taccaacaat tcctgcagtt cctgatccac actatgattt
     3601 aattattacc attgtagcat tttcaccaga agagtccggt gggtttacct atggccttga
     3661 taccagtttt agtttaggac tcataccgta tgtacggtat acccataatg atctcataca
     3721 gcggggcgat agtttccgga taggttttaa aactggtttt atgtacgggc tggatggaaa
     3781 ttttacttca cttccctaca acacgtttaa tcagataaaa tccacctata acttcagatc
     3841 acactttgat ggcctgttta tccccagggt ttcatctgaa gtgtacaatt caaaagccgg
     3901 gcgaaaggac ttagggcttg accagtatag gtatgtaagg gtaaacgttc tggccgaacc
     3961 aggatttacc cccattgaga aattgaaagt atatccggga tatggcgttg aaaaagtttt
     4021 tattttgcag gctgttaaaa accctgatac tgcagtaaca cagcccaaca tagcaaaaga
     4081 tacgcaggtg tggcacttta ttggcattaa agcaaacatt gaccttattc cattttcgct
     4141 aaaacataca acagggcgta agataaccct tgaagcctac cgctatcaaa atgcaaccca
     4201 tttttacaaa gtgggcattg atactattgt taaatttgaa ttttttaact ttgatttgtt
     4261 tgttttccag ggtgattata cctatcttaa tgacccggtg cctttttatt atgaggaacc
     4321 tgtttctaca cggacgttca aaggattcat gggcaaggat taccatacaa cgcatattgc
     4381 acgtgcttct tttgaatacg aaatttctat acacagggat tttatatata ccggtgtgta
     4441 caatgacttt actgctttta aaggcaaata tcttttacat ggcaatcaga taggcattgc
     4501 ttacggcctg agttttcatt acgtattttt tgaacaattt gaatttgcac tatggtgggg
     4561 caaggattat cttccttcca ctgaccagtc ggggtacaac cttaaattta cattggccaa
     4621 gaaatggtag gtggtatttc atgcgtgttt gctggatgca tattgggtac tggctaaaaa
     4681 atggcaaggg ctttttcatg cgtgtttgct gcacgcatat gcatagtttc acagtaaccg
     4741 tcaaactaac cgcaggttag atgaatctat atcaatttta aaaacgcaag aaaaatttat
     4801 tttcgtactt tactttcagg taagcatata tcattcaacg aataatatta agatacatag
     4861 cacttatgct aatgtatccc ttaaccaccc tcatttactt ttacctgcat gccacctctg
     4921 tctattatca cgggatattc ctttttggta tttgttgtat gtataatggt gtgagctatt
     4981 gcaacacata gactgctatt atccttaatc atcaagcaaa tgctgatacc accgtgcaaa
     5041 ttggatataa ttcagattta tatcataaat tactattatt ttatcacctg aaacaaacca
     5101 ataatgactc ctattatggt tattgtttgc gttagcaaca accctataag ccattttaac
     5161 aggcttacct ttgtttgctg tatttcaatt tttaactcgg ctcgcacccg ttctatctct
     5221 tttgttaact taagctctat ctcttttatt tccagacgca ttttctctat atcctttgta
     5281 aggtcaacac gcacctgttc tatctctttt gttagcttca gctctgtctc tttcatatca
     5341 gctcgcacct gttctatctc ttttgttaat ttcagctctg cctcttttat ttccagacgc
     5401 gtttgctcta tctcttttgt taactttaat tcctggacat gtaaatcctg ccttgttgcc
     5461 acctgattgt tatttattgc ttcctctacc ttttcaataa attcggccaa gacttttgcc
     5521 tttacttcat catccttaaa ggcttcatat acccgcattg caaatcccat tgtactatac
     5581 ccttccttat gtattttttc tacgaatcat actctattca ttctacagac tttcttgtta
     5641 tcagtttttt cccatacagt aaatataatc tacatgcaat acctgcgtca ataaaattta
     5701 tttttttcat aattcctggc attgcggata taataatact cattgcccat ttcatgatta
     5761 aaatacatga aatctcttta ttaattttct gcacttattt aaaaattatt ttaaattttt
     5821 cccaactcct gcacaattac atctaaaatt cataattcaa ttttttgaat aattcaatgg
     5881 gtataattca cgtatgatat atatatattt acaggggtgg ctaaggctcc cgaagccacc
     5941 cctgttttat attacggtcg tttcgacagg ctcagcacag gcgtttcgtt aggctcagtg
     6001 accggtcatc gagtagcact acaggaatgt atcgagacaa ccgcattgta ttataatgaa
     6061 tatatttcat aaccagatgg taaggtttaa tacaccgcta ctatacgagc tgtattgaaa
     6121 taacggattt catttcgcag ataacagtat gcctgtatgt agttgttttt tcccattata
     6181 aagtagcgct gtggcagtat gctgcgctcc acaatattcc cctgtgcatc tttatatctg
     6241 atagttatac ggtaccccaa gggtatcctg ctatcggcag gaatatgtgt gctttttatg
     6301 ccaagtgaat aggcactgat aataccacca tgatagcgtt ccagatcacg aatacaccag
     6361 ttgtgcgcat tgagcttatc aatgaccttt ttaaatattt ccattgatcc ctgtgcatcg
     6421 ctcagtgcgt ggtgagcatt aatggatata ccaaaaaatt tacaaagatt gcctaaatta
     6481 tagcttggaa gtttcttaaa tgtcttgcgt gctaaatgta ctgtatcaaa tgacttcaaa
     6541 tctggagtat ttctgtcatg ctgatggtat gcagaatcaa tgaatgatat atcaaaacgt
     6601 ggattgtgga taacacagta ggtattgctg gtatattgtt ctatagtatc aaataattct
     6661 gtaataaccg gagctttctg taccatgtca gcagtaattc catgaagccg tgtaacatgt
     6721 ggtggaattg gcttcaatgg gtttaccagt gtatagaatg tgtcaatgac tctgtctaat
     6781 gtaaaccgta ttattccaat agaaactatc ctgtcaatga tgggatttaa tccggtagtt
     6841 tcacaatcca gtgcacagaa tacggtatca tgtaatagtg ttgcaaattc catgtatatt
     6901 aaaattcccc tataaaacgt atttcaggct ctaaattgat atcatacagt ttatgcaccg
     6961 tttcctgaat aaattcaacc agatgcttga tatccatagc agtggccttg ccagtattta
     7021 caataaaatt tgtatgtaac tctgacactt tggcaccgcc aatactgtac ccgcgtaaac
     7081 ccgcatcgtt gataagcttc catgaagcat gcccgtcagg atttttaaag actgatccag
     7141 ccgaaggata atccagcgga tgcttgtttt tgcgctcacg caaaatctct tttacagtat
     7201 tttttacatc ctccgggtta tctgcaatat ttaatgtgca ccgggcactt ataacaatgg
     7261 caccttccgg caacatggat tttcggtatg aaaataacga cgtgtttgca acagttaaca
     7321 gcgtgccatc tgcggtataa caggttatat cggtaataat atctgaaaaa gtccccatag
     7381 tggtaccagc attcatgatg acaccaccac caatacagcc tggtatccct gcaataaact
     7441 ccatgccagc aagcccatgc tctattgctt cttccacaaa tcgttttttt gttaccttgg
     7501 catcaaccac aacgtttgta ccgtcaaatg caatggaacc ttcatcttcc tgtgaacttc
     7561 ccattctcac aacaagccca cgtatacctc tgtcacccac cacaaggttg gaaccgcctc
     7621 caataatggt aacaggcgtg ttagtttgtt tggcgatagt tactatgtaa gggattgttt
     7681 tattggaaca aggctcaata agcatatcag caggtccacc aaccttaaag gtggtaaatt
     7741 gactcatagg aacatcaacg tacagcctgc catactgcga cagttccttt ataacatcat
     7801 gtgaacaggt ttgtcccaat ggtatttatc tcctctggaa ataggatttt atataataga
     7861 tgtcattgca atcccaatat aatcagttat gtctcgatac aattactata caatcactcg
     7921 acgaccgcat cttgatagat ccttcgctcc gactttatcg ggactcagga taaaaaatac
     7981 ccaggtcatt ctgaggacgc cccaccttta ttggggcgga cgaagaatct tgacgtttcc
     8041 gttttacaca cagtcattgc tatttacatg agactgttta ccttcaattc agttcagagc
     8101 aatcacttta tttagtaatg aaaatatttg catcatcaac aaaagttacc atcaccgcat
     8161 ctacaaccat ggacaacgtt tatataacaa atattaaata ccaccttcat tgatattttg
     8221 cgatgaaaat caacaatatt tttaaaaaat taactatgag gaggaatgtt atatcattat
     8281 cacctgcaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna atatttttaa
     8401 aaaattaact atgaggagga atgttatatc attatcacct gcaattcttc agcagctttc
     8461 tttgctccat cagtatattc atcaccaatt accaccggta ttccaggtat tcctaaacac
     8521 ttttcaatga tttttgttct tctagctgct ctttcaacat cggttttatc cacaacaatt
     8581 gaaacctcag cagccaaaaa aacctttttt tctttattgt gacgcaaaaa accagtaaca
     8641 accacatcaa ctttcagtgc atcatcacgt tcatcatcag ttatagtggc ttttttgttc
     8701 agtatagcat catcaatact atttgccaat tccatggtat caattaatct gcattttaaa
     8761 atgagttttc caaaatacga aggtgctttt tcttttacct tccgttcaaa attgtctcct
     8821 tttaggcttc caacatcatt accctgtttt tcaacagttg cttttaaggg cttaaattca
     8881 ttttctataa agctttcata gcgttttaca aattcatcaa attttttagg cagtgcaagc
     8941 agttcctcag taagaataag tttcctgagt tcttctaacc aatcaggatg ttccttcaaa
     9001 actgaaagta aatcccaata cgacttaatc tttgtttttt tcatctcttc catggtttta
     9061 aaataagcat ccgatgatgt tttgtcaact atcatatact tctctcctgg tacacatgta
     9121 tctttattta ttactttttc tttttaattt ttgctgcagg ccttgttata ggcttttttt
     9181 tcactatttt atgctcttct tttctaaccc cattttccag aatttttcca ccttcaagat
     9241 gatactgcac attgcttgcc tttacaatac gttcatcatg ggttgctatt attattgata
     9301 ttcccttgtc atcacgcaag cgcgcaaata gcttcaatat atcattggct gtggcagtat
     9361 ccaagttgcc ggttggctca tctgcaagta ttaattttgg ttcattaatg attgcccgcg
     9421 ctattgccac acgctggcgt tcaccacctg acaatgtggt aggatttctg tccagtatat
     9481 cggtaatctg caggtattct gccagttcca ttatacgcgc atgtaaatta cgcgtttccc
     9541 ggttaagaat tgccgggaat gcaatattgt catacgccga aatatcagaa aaaagattaa
     9601 aaaactggaa gacaaagcca atatccctgt ttctgaaacg cgaccgcttt aagtcactcc
     9661 atgtaaaaat attatcaccc ataaaaagta ctgtgccttc attcggctgc aataatcccg
     9721 atagtacacc tagaagagtt gttttccctg aacctgattt acctgttaat gcagtgatag
     9781 tgctttcctg aacttcaaag cttacatcat ccagaacttt gcgctcatca tactgaacac
     9841 ttaccgattt tacctgtaat aatgccacgg ttatacctca ctgtttgctt aatgctttaa
     9901 ataaattcat gtttgacgca aaaatagccg gtatcaatgc tgacaatgat gaaattgcaa
     9961 ttgagaaaag aacaagacca gcacatacat tcatgcctac tggctcaagg ccaataccgc
    10021 ttaatgccgg caccagtgat gaaagttttg ctccagcagc ctttattaag tatgcacctg
    10081 ctagataccc aatgaaacca tacactgcac caataaaagc tgcttcaacc atgaatgtca
    10141 tgattattct gaattttgat actcctaaaa tcctgcgtag tgaaaagctg taactccggt
    10201 tatatatgat tattaaatat gaattaaaaa ttgatataac agtcagtact aataagaaga
    10261 aaccaacaaa gattgaaaat ccatcaataa tgctcatggc ctgatttgtt ttgcttgcca
    10321 cttcatcaag ggaagtaacg gcaagcccca tggacttgat ttgttttgta acttccggta
    10381 gttttttgac atctttcact tttacataca atctgatgta caggattggc tgagcacttt
    10441 tggtttctgc agcataccgg gctgctattt tattaacaaa tgtatcaggc acaacaatac
    10501 ccgttaaatt gaaaagcgtt gaaaatgaat gaaccacagc cggtacttca aagcgctttc
    10561 ttgtttcagc accttttggg gtattaatta ttaaggtaag tggaaatcct tttaaggtgt
    10621 cttcattaaa taaaggaaga ttgttgattg cagcaaaatt gttaaacata tccagtgcca
    10681 cctgtggggc tatcactgga accggctcat tggtgtaata tctgaatgat ttataccctg
    10741 gcactttatc tttgataaac tgctgactta tcccacaaat gggtacatag ggtgcccgtg
    10801 atttccctaa taattcaatt tttatctttg ttgctgcatt tatacaggta acagtataca
    10861 ccgatgcaac agtattcatt ttacggatag ctttaacttt ttctactgga atgctattta
    10921 tagcatacga tagtgttgct tcaccagttt ttgcttttgg ttcaacgatt atttcgttaa
    10981 tatccagtga gccaaaaagc ctcttttcta caaaattcgt aacagaatcc ctcagtgaaa
    11041 gatacagtac taaaaaggct ataagcaata tgatgccaaa acttgacagc aaagccttcc
    11101 atatattttt aactatctcc tcaataatta attttgcatt tcgaataatg tactgtacca
    11161 taatgatgta ctacctcatc aaagattgta taaaacatta ctatattttg tgttactaaa
    11221 caaaaaatta tgcaaatata aaaaaattat tgcattttta ctaaatatca taaatatctt
    11281 cataaaacaa taacaaaatt tatgcaatga aataaagata ttatttatgt caaataaaaa
    11341 aatattaata tacctattgt ttattattcc tttcctttgt tgtgcaacat atacccctgt
    11401 agaaaaaata tatgaagata ataaaattgt taaaatagtt atacgagata aaaaaggggt
    11461 aacaggatgg tatattatta attacgatac aaacaacaga atcacaggcc ttaataaata
    11521 tacaccgtat aaaaatactc ctattacaaa aattcagctt caatacaagg gcaagctttt
    11581 aacgagctac acaattgcat cgacatctcc cgaaagtgat acagtgttgt accaatcaac
    11641 acacatattt aattactcat atgctaatga tatcaggcga gttgatattt tattcaataa
    11701 accggcaagt ataaagcagg caggaacaac ttttatcaga attcaatatt catacactga
    11761 taaattagta acaggactat atataaccgg tgatgcgtca agctttaaga tttcctgtac
    11821 tataagctat cgcaaaaata ctattgatgc aattactatt gatgaaaagc gatacaatac
    11881 agcaacaaaa aaatttgaac agggtttaaa aggcacgtta tatattgata aaacaccaga
    11941 gtatttcatt gatgaagcaa ccggtaataa aattaaaaac aaagacaagc ttacttctat
    12001 atatgccaaa acatatatac aagatgctct taataaactt acattgacaa atggcaatga
    12061 acagataatt gattattttt tattgtcaga tagttccttg taatgtaaac gtttttttat
    12121 ttgcaaattc aagaataaag tgcaatacaa aatagaaaac atgtagtctc atacatctaa
    12181 agtctcacag ggaaaagaat gtaccaccag cataagaagt gtaactatat aaataaataa
    12241 gtgataatcc aaagcaaagc gttctttgaa aagagcaaag taaggagcaa tgatagccgt
    12301 acaggaggta cggcgtaata tgttgcgtgc gtggttcgac aggctcacca accgtgcttc
    12361 gacaggctca gcaaccatgg tccgacagga ggtcactaag cttgtcgaag tgatcaccat
    12421 tcttcattag ataggaacag tacaggcgcc gtatttttg
//
LOCUS       DAORYL010000089        12299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_4006_length_12299_cov_12.911303, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000089 DAORYL010000000
VERSION     DAORYL010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12299
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4006_length_12299_cov_12.911303"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PLV81_13000"
     CDS             complement(<1..257)
                     /locus_tag="PLV81_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11055.1"
                     /translation="MKRIIQVTLYLNGHCVETAAKETLQKLLDAMLQSETEDQNLHER
                     YQLLYDFLHTADFKQLRASDERLTGIVPTVCEVYRDENGKPA"
     gene            416..712
                     /locus_tag="PLV81_13005"
     CDS             416..712
                     /locus_tag="PLV81_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013798425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOM11056.1"
                     /translation="MKSTHEVIAILSSNKDVLKEKYGVMNIAIFGSYSREDYNQDSDV
                     DIIVEFEKPIGYKYFELADYLESILQCKVDLFTYNAIKQKPLLWESVKEDIVHV"
     gene            705..998
                     /locus_tag="PLV81_13010"
     CDS             705..998
                     /locus_tag="PLV81_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOM11057.1"
                     /translation="MFKRNYLLFIEDILQNIIKINKYINNLGYDEFLDDDKTKDAVIK
                     NLIEIGEAANHIPENVLKQYNEVPWQQMIGLRNRLIHGYFVIDYDIVWTIIKE"
     gene            complement(1066..1407)
                     /locus_tag="PLV81_13015"
     CDS             complement(1066..1407)
                     /locus_tag="PLV81_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOM11058.1"
                     /translation="MRIALPLTEDNNFSMHFGHCDKFVLYDIDETNKHIIKKEEIPAP
                     PHQPGLLPRFLAEKGATCILAGGMGTRARQLFEEQNVVVITGVEGTEPDTIVNDYLQG
                     KLKTGANMCDH"
     gene            complement(1409..2299)
                     /locus_tag="PLV81_13020"
     CDS             complement(1409..2299)
                     /locus_tag="PLV81_13020"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOM11059.1"
                     /translation="MFGKGKDGSKAQAVKPGMDSKQVTEINEELKLQENLNKIKHTIL
                     VLSGKGGVGKSTVATNLAVALASRKFKTGLLDIDIHGPSVPTLLGIEKAPLHATDHNT
                     IVPVQYNDFLKVISEGLMLPDTKEAVIWRGPLKYNLIKQFLSDVEWGELDYLVIDSPP
                     GTGDEPLSVAQLVKNPTGAVIVTTPQNVSTIDVKKSITFCHKLNIPVLGVVENMSGFV
                     CPHCGKETYIFRKGGGSEIATEMDVPFLGTIPLDPLIVENSDAGRPFAQSAAVNATVS
                     QKHFEAIVTALLQSIKSKEA"
     gene            complement(2331..3218)
                     /locus_tag="PLV81_13025"
     CDS             complement(2331..3218)
                     /locus_tag="PLV81_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOM11060.1"
                     /translation="MKPSIQQLAIVSGKGGTGKTSIAASFAKLAKRRIVVDCDVDASN
                     MHIVLPHTIIQEIPFFGEKKAVISQDRCRGCGDCVIYCRFNAIKDFYVVEPVLCEGCA
                     VCAMMCPYEAIDMLDNQSGTVVISQSDGQPFLYANMGIGEGNSGKLVSELREKAKSIA
                     EEENYDLIIIDGPPGIGCPVIASITGTTHVLIVTEPTISGIHDLERIVLLVKHFRIPS
                     SVCINKSDISEENRQLIMDICKKNNIPVIGTIPYDEDVIRAQIAGKTVVEYSDGSASQ
                     EITKLWQTVQTMLQATIAK"
     gene            complement(3215..4060)
                     /locus_tag="PLV81_13030"
     CDS             complement(3215..4060)
                     /locus_tag="PLV81_13030"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM11061.1"
                     /translation="MNIAIASGKGGTGKTTLSATLAFYASQFQTTALLDCDVEEPDAN
                     LLLKINVENRYPVNVLIPQVDMDACTGCGKCQKVCQYNAIVLIKARPLVLPELCHSCG
                     GCYLACPEHAIKEITRPIGTIETGNSNNLFYAAGVLNIGEHITTPLIDEVKKEHRHAT
                     LRIIDAPPGSSCPVIHAMEHSDFVILVAEPTPFGFHDVVLAYQVAKSLSIPCGIVINN
                     MVEEYEPLRNFIHNENIPLLATIPHDINIARSTTQDNFIDYMISHYGQHIQNIYEYCT
                     NRVIL"
     gene            complement(4079..>4913)
                     /locus_tag="PLV81_13035"
     CDS             complement(4079..>4913)
                     /locus_tag="PLV81_13035"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOM11062.1"
                     /translation="ITVVCENSIGTPFPLIGEHGLSVLIEADDTTLFDTGQGLGIIHN
                     LKTLGKDITAIQRIILSHGHYDHTGGLLQVLQSINRNIDVYAHPDIFTNKVAIIPLGN
                     QTLEAPIGVRASKEEYEKAGAVFKPISGLTAITQSIRAIAEINRPQSWQGFDERLKVK
                     LPDGTIKDDIFNDDCSLVIDTESGPVVLLGCAHAGIVDILYDIAKQTGYKEFHAVIGG
                     THLESAPDTYVQKAIDTLKHFKVKKIGTSHCTGFKMACRMHQEFNESFVLATCGAVFE
                     F"
     assembly_gap    4914..5013
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5014..5345)
                     /locus_tag="PLV81_13040"
     CDS             complement(<5014..5345)
                     /locus_tag="PLV81_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOM11063.1"
                     /translation="MPRGDRTGPYGAGPMTGRGAGYCAEYGLPGYANPAGRFGASGIG
                     RGFGGRGRGFRHWFYATGLPGWMRFGYNAPAPYQYSKDDEKSFLKEQVDFLSKTIENL
                     NKRLAELE"
     gene            complement(5431..5898)
                     /locus_tag="PLV81_13045"
     CDS             complement(5431..5898)
                     /locus_tag="PLV81_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11064.1"
                     /translation="MNQSTLHNGFTGYTQNVQEADRIMRERMLQCIKKLHEKNLHCLN
                     EAKSHGKEKLFSEIETMHKRLEKFEQEINKQPIAHFKLEGLDQSDEDKLKMIEEKIER
                     LAQECGDIIDNLSCSQVDMHIIEQYTRIASHLRLAERLYHERMMILKKAMVFG"
     gene            complement(5935..6294)
                     /locus_tag="PLV81_13050"
     CDS             complement(5935..6294)
                     /locus_tag="PLV81_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOM11065.1"
                     /translation="MKIAIPARQNSLDSEVDERFGRAKGFIIYDTDTETHTWVDNKQS
                     VQSTQGAGIQSAKNVIDSGAEVLIAGNVGPKAYSVLRASGVEVYVCDVMPVSQAIAEY
                     KAGKLIKASEATVESHW"
     gene            complement(6263..6691)
                     /locus_tag="PLV81_13055"
     CDS             complement(6263..6691)
                     /locus_tag="PLV81_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11066.1"
                     /translation="MKNEIIALPVFNDRLSPLLDEARKFIVMTLNDVTISERTVIEIN
                     EQSAFIRIERLKEMGVTVLLCGALSDVLARFILDREIQLYSWLNGTIDEVVEQYINGA
                     LPNKCMRYVTKTRTCRRQRRGWKLHQRRNHYEDSNTSTTE"
     assembly_gap    6842..6941
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7282..8589)
                     /locus_tag="PLV81_13060"
     CDS             complement(7282..8589)
                     /locus_tag="PLV81_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOM11067.1"
                     /translation="MMNSDCVANIILDSIADGVFTIDMDFRITSLNNAACKILGLESK
                     DEAIGKLCFEIFHANICEHSCALRETMQTGKNIINKTVYIVNKSGEKVTISISTALLK
                     DDQGNIIGGVETFRDISDIETLRKKIESTYSFEDIISKNKEMQQIFSILPDVALSDSS
                     VLIEGPSGSGKELIARAIHSLSRRKEKPFVVVNCGALPENLIESELFGYKTGAFTDAK
                     KDKPGKIAMAEGGTLFLDEIGELPLSMQVKLLRFLQFKEYEPLGDTKPKKANVRIIAA
                     TNKNLREEVAAGNFRDDLYYRLNVIGLRLPSLSQRKEDIPLLVEHFIKKYNTLKGKSI
                     EGVSDDAMNILLNYDYPGNIRELENIIEHAFVLCHEAYIKLEHLPAYVYGITPVSTLS
                     LEEMEKTMIKQALARYSNKKDAAAALGIDTSTLWRKIKKYNLE"
     gene            complement(8586..9425)
                     /locus_tag="PLV81_13065"
     CDS             complement(8586..9425)
                     /locus_tag="PLV81_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11068.1"
                     /translation="MKKFYIIAIISCILTIGCFDFDIVHYVEPKKDRSLFIKFRVTSI
                     YFKDQTDKKTFNNDVIKGNLPDEKDTQVTYQWIENDLTAGVEITTTAKPHSTISATDK
                     FPVIPYRDKFGQYIFIYHNYQELGSSQNLFDSQKIAEGLLAASKYRLAFGNTVPKQAL
                     IIVYREQPEKFNLSIYQLGNIYCIDIPMNLIIMNESAVIVSCASTINDSEISNYLTKL
                     LEKRKEEEKKHEELKKQENNENDATNNYNDSNDEDNSDTQFDNNHNNDEHENIDNDTD
                     NVE"
     gene            9941..11566
                     /locus_tag="PLV81_13070"
     CDS             9941..11566
                     /locus_tag="PLV81_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007084251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOM11069.1"
                     /translation="MMRYPLLLTNFMERAAKYFPKKEIVSVYPNEKFVYNYGEWYKRC
                     CQLAHALQSLNIKKGDRVASFALNNHRHLELYFGVPCMGAVLHTLNVRLSHEHLVYII
                     NHAEDKIIFVDEDLYFLLEPVKDKLKTVKHYVILSQSGKMPETKLSPALLYDDLIKDF
                     PGEFEFPKDRDEYDPALICYTSATTGDPKGVVYSHRGLVLHAYAAGVTLGLNESDCAM
                     HVVPMFHANAWGGPFGATGLGCKQVLPGRQILDMKALCKIISEEKVTFTCGVPTIWMM
                     LHQYLESGGEHDFSSIRAVYSGGAAMPRYLMESFAKKYNFNIVQAYGMTETSPLATAA
                     IPKSYMQNLSDKELFDIKTTAGLLALGLEMKVVNLETGKEVQWNGKEMGEVCLRGPWI
                     ASEYYKDPERSKQTFKDGWLHTGDIATINEEGYISLVDRTKDLIKSGGEWISSVDLEN
                     QIMAHPKVLEAAVIAMPHEKWQERPLACIVPKPEFADSITKEDIMEFLEAKVAKWWLP
                     DEIVFLKEIPKTSVGKFNKKELRKTVLPEVLKK"
     gene            complement(11576..12250)
                     /locus_tag="PLV81_13075"
     CDS             complement(11576..12250)
                     /locus_tag="PLV81_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11070.1"
                     /translation="MILAINIQLYAKKFLYLVLLFIFYFSLYTSIHAQSSNGNYSFGI
                     NVAGGMNDVLHRSGSEEGKTVKWKPGLIAGGGIFFENIFSSHFGIHSGISYAFHETYL
                     EFGDTPDAQITSQNHSIIIPLYLISSFGNKVRLDILYGLNYMHIFHITMSNDNDSTNG
                     IRFINYNQFGCGLQLRLAFAFNKFTYLYAGPHGQFYFSNVIQDTNWRDYLYNIQLEIG
                     MLFKTF"
BASE COUNT         3676 a         2275 c         2109 g         4039 t
ORIGIN      
        1 gcaggcttcc cattctcatc cctatatacc tcacatactg ttggaactat accagttagc
       61 ctttcgtctg aagctcgcaa ttgcttaaaa tcggcagtat gtaaaaaatc gtacaaaagt
      121 tgataccgct catgaagatt ctgatcttca gtttcagatt gtaacattgc gtcaagaagc
      181 ttttgcaatg tttcctttgc agcagtctca acgcaatgtc cattaagata taatgtaact
      241 tgaattattc ttttcattat gttgttactt aataactaac tctttaaatt aaaaaaatga
      301 tgaaaaatca agtttatttc aataatagaa aaatataatt gacgttacat atactatacg
      361 tatattttaa aatatatatt aaataccgta aataagtttt ttataggttt tcgcgatgaa
      421 atcaacacat gaagttattg caatattgtc atcgaataaa gatgtattaa aagaaaaata
      481 tggagttatg aatatagcta tatttggttc atattcgcga gaagattata atcaggatag
      541 tgatgttgac attattgttg aatttgaaaa accaattgga tataaatatt ttgaattagc
      601 tgattattta gagagtattt tacaatgtaa agtggatttg tttacatata atgctataaa
      661 acaaaaaccg cttttatggg aaagtgtcaa agaggatatt gttcatgttt aaaaggaatt
      721 acttgctttt tattgaggat attctccaga acattattaa aataaacaaa tacatcaaca
      781 atcttggtta tgatgaattc cttgatgatg acaaaacaaa agatgccgta atcaagaatt
      841 taattgaaat tggagaggca gcaaatcata ttcctgagaa tgtacttaaa caatataatg
      901 aagttccctg gcaacaaatg atcggattac gaaatagatt aatacacggt tattttgtca
      961 ttgattatga tatagtatgg acaattataa aagaataatt gcccatacta tttgaaaaac
     1021 tagagaatat aaaaagaagc caataacata tttttacaca tagtattaat ggtcacacat
     1081 attggcacct gttttaagtt ttccctgtaa ataatcattt actattgtgt caggttcagt
     1141 gccctcaacg ccagttataa caacaacatt ttgctcttca aaaagctgac gtgcccgtgt
     1201 tcccatacca cctgcaagga tacatgttgc acctttttct gccagaaatc gtggtaacaa
     1261 tccgggctga tgtggtggtg caggtatctc ttctttttta atgatgtgtt tatttgtttc
     1321 atcaatatca tataacacaa acttgtcgca gtggccaaaa tgcattgaaa agttattgtc
     1381 ttcggttaat ggcagtgcaa tacgcatgtt acgcctcctt tgacttgatt gattgtaata
     1441 gtgcggttac tatcgcttca aaatgctttt gtgaaactgt tgcattgaca gcagctgatt
     1501 gggcaaatgg tcttcccgca tcactgtttt caacaattag tggatctaat ggtattgttc
     1561 ctaaaaatgg tacatccatt tcagtagcta tctcactgcc accacctttt ctgaatatgt
     1621 atgtttcttt cccgcagtga ggacatacaa atccactcat gttttccaca acacctaaaa
     1681 ccggtatgtt aagtttgtgg caaaatgtaa tggatttttt tacatcgata gttgaaacat
     1741 tctgtggtgt tgtaacaata actgcacctg ttggattttt tactaactgc gcaactgaaa
     1801 gtggttcatc accggttccc ggtggtgaat ctatgacaag ataatcaagc tctccccatt
     1861 caacatcgga taaaaactgt ttaataaggt tgtattttaa aggacctcgc catattaccg
     1921 cctcttttgt atcaggcagc ataagtccct ctgatataac ttttaaaaag tcattatact
     1981 gaaccggtac tattgtatta tgatccgttg catgtaacgg tgctttttca ataccaagaa
     2041 gagttggtac gcttggacca tgaatatcaa tatccaaaag cccggttttg aattttctgg
     2101 aagcaagtgc tacagctaaa tttgtagcaa cggtgctttt gccaacacca cctttacctg
     2161 ataaaaccag tattgtatgt tttattttat tcagattttc ctgtaatttg agttcttcat
     2221 tgatctcagt aacctgtttt gaatccatac caggtttaac tgcctgtgct ttagaaccat
     2281 cttttccttt accaaacatc tatagcctcc tttaaacgtt attgaatgta tcatttcgct
     2341 attgtagcct gtagcattgt ttgcacagtt tgccatagct ttgtaatttc ctgtgatgct
     2401 gaaccatctg aatattctac cactgtttta cctgcaattt gtgccctgat gacatcctca
     2461 tcatacggaa tagtgcctat aaccggtata ttgttttttt tgcagatatc cataattaat
     2521 tgcctgtttt cttcagagat atcactttta ttgatgcaca ctgaagatgg gatcctgaaa
     2581 tgcttgacga gcagtactat acgctctaag tcatgaatac ctgaaatggt tggctccgta
     2641 acaataagta catgtgttgt accagttatg gatgctatca ccgggcagcc aattcccgga
     2701 ggcccatcaa tgatgataag gtcatagttt tcctcttctg caattgattt tgccttttca
     2761 cgcaattcgg aaactaattt accggaatta ccttctccaa tgcccatatt tgcataaaga
     2821 aatggctggc catcagattg tgaaatcaca acagtacccg attgattatc aagcatatca
     2881 attgcttcat aagggcacat cattgcacac acggcacaac cttcgcataa caccggttct
     2941 accacataaa aatccttgat tgcgttaaat ctacaataaa taacacaatc gccacaacct
     3001 ctgcatcggt cctgcgatat aacggctttc ttttcgccaa aaaagggaat ctcctgtatg
     3061 atggtgtgag gaagcacaat atgcatgtta gatgcatcaa catcacaatc aaccacaatg
     3121 cgccgttttg ccagtttagc aaatgaggcc gcaatacttg ttttgccagt gcctccctta
     3181 ccactaacta tcgccaattg ttgtattgat ggtttcatag gatcactctg tttgtacagt
     3241 attcataaat attttgtata tgctggccgt aatgggaaat catatagtca ataaaattgt
     3301 cctgtgttgt ggacctggca atgttaatat catgcggtat agttgctaaa agagggatgt
     3361 tttcattgtg aatgaagtta cgcagtggct catactcttc aaccatatta tttatcacaa
     3421 tcccacatgg tattgataat gactttgcta cctgatacgc taatacaaca tcgtgaaaac
     3481 caaacggtgt tggttcagcc accagtataa caaaatcact atgctccatg gcatgtatca
     3541 ccggacatga agaacctggt ggggcatcta tgattcgcaa agttgcatgg cggtgttcct
     3601 ttttaacttc atcaatgagt ggtgttgtta tatgctcacc aatattcagt acaccagctg
     3661 cataaaaaag attgttacta ttgccagttt caatagtgcc aatgggtcgt gtaatttctt
     3721 ttattgcatg ttcagggcag gctaaataac atccaccgca tgaatgacat aactctggta
     3781 ataccagtgg ccttgcttta ataagaacta ttgcattgta ctggcatact ttctggcatt
     3841 taccgcaacc agtgcaggca tccatatcaa cctgtggtat gaggacattg acgggatatc
     3901 tattctcaac attgattttt aatagcaggt tagcatcagg ttcttcaaca tcacaatcca
     3961 gtaacgcagt agtctgaaat tgtgatgcat aaaaggctaa cgtagcagat aatgttgttt
     4021 tacccgtacc tcctttgccg cttgcaatag caatattcat acactatctc caataccttt
     4081 aaaattcaaa aacggcaccg caggttgcca gtacaaaaga ttcattgaat tcctggtgca
     4141 ttcgacatgc cattttaaat ccggtacagt gtgatgtgcc aatcttttta actttgaaat
     4201 gcttcagtgt gtctatagct ttttgcacat atgtatcagg tgcgctttcc agatgagtgc
     4261 caccaataac agcatgaaat tctttataac cagtttgttt tgctatatca tacagtatat
     4321 ccacaatacc tgcgtgtgcg catcccagaa gcacaacagg accagattcg gtatcaatga
     4381 caagcgaaca atcgtcattg aaaatatcat cttttatggt gccatcaggt agtttgactt
     4441 ttaagcgctc atcaaatccc tgccagcttt ggggcctatt tatttcggcg atagctctta
     4501 ttgattgggt tattgctgtt aatcctgata ttggtttaaa aacggctcct gccttttcgt
     4561 attcttcttt tgatgctctg acccctattg gtgcttccag tgtttgattg cctaagggaa
     4621 taatagcaac tttgttggta aatatgtccg gatgggcata tacatcaatg tttctattta
     4681 tagattgtaa tacctgcaaa agtccaccgg tatggtcgta gtgtccatgg cttaatataa
     4741 ttctttgaat tgcagtaata tctttaccta atgtttttaa attatgtata ataccaagtc
     4801 cctgacctgt atcaaaaagt gtagtatcat cagcttcaat taataccgaa agtccatgct
     4861 ctccaattaa tgggaatggt gtcccaatgc tattttcgca tacaacagtt attnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttttcca gctctgccag gcgcttgttt
     5041 aaattttcaa tggttttgct taaaaaatca acctgctctt tcaagaatga tttttcatca
     5101 tccttagaat actgataggg tgcaggtgca ttgtaaccaa accgcatcca tcctggcagg
     5161 ccagttgcat aaaaccagtg acgaaaacct ctacctcttc caccaaaacc tctgccaata
     5221 ccagatgcac caaacctacc agcaggattg gcatatcctg gcaaaccgta ttcggcgcaa
     5281 tatccagcgc cacgtcctgt cataggacca gcaccatatg gtcctgttct atctcccctg
     5341 ggcatactgt tacctccttt tatacaaaat agtaatgtaa tgcaataaaa cgtttcaaca
     5401 gtaactatcg cacataattg ctatctggtc ttaaccaaat accattgcct tttttaaaat
     5461 catcatacgt tcatggtaca atcgttcagc caatcgtaaa tgcgatgcaa tgcgtgtata
     5521 ctgttctata atatgcatat caacctgaga acaggaaagg ttatcgatta tatcaccaca
     5581 ttcctgtgcc agtcgctcaa tcttttcctc aatcattttc agtttgtctt catcagattg
     5641 atcaagacct tccaatttaa aatgtgcaat gggttgttta ttaatttcct gttcaaactt
     5701 ttccagccgc ttatgcatag tttcaatttc agagaacaat ttttcttttc catgagattt
     5761 tgcttcattt agacaatgca aatttttttc atgtagtttc tttatgcatt gaagcatcct
     5821 ttcacgcatt atcctgtcgg cttcctgtac attttgtgta tatcctgtaa aaccattatg
     5881 caatgttgat tgattcatat ggctcacctc ctttaaaata aatgtaaaaa attattacca
     5941 gtgactttct acagtagctt ctgatgcctt aattaatttt cccgctttgt actctgcaat
     6001 tgcctgtgat accggcatca catcgcacac atacacttcc acacctgacg cacgtaacac
     6061 tgaatatgct tttggtccaa catttcccgc aataagtact tctgcaccac tatctatcac
     6121 attttttgcc gattggattc cagcaccctg cgttgattga acactctgct tgttgtctac
     6181 ccatgtatgg gtttccgtat cagtatcata tataatgaat ccttttgctc ttccaaaacg
     6241 ttcatcaacc tcactgtcta aactattctg tcgtgctggt attgctatct tcataatgat
     6301 tcctcctttg atgtaatttc catccacgcc tttggcgcct gcatgttcgt gtttttgtta
     6361 catatcgcat gcatttgttg ggcaatgctc cattaatgta ttgttctact acttcatcaa
     6421 tggtgccatt cagccatgag tagagctgaa tttccctatc aagaataaac ctggccagca
     6481 catcactcaa tgcaccacat agtaacactg taactcccat ttctttaagg cgctcaatcc
     6541 gtataaacgc actttgttca ttaatttcaa ttactgtacg ttcggaaata gttacgtcat
     6601 tcagtgtcat aacaataaac ttgcgcgctt catccaatag tggcgacagc ctatcattaa
     6661 acactggtaa tgcaataatt tcgttcttca tacttaataa ttaagcataa atcgtgccaa
     6721 tatgcgtaat tgcagcacat tcccacaagg atattttttt tgtgcgatag ttactgtaca
     6781 aaagcttatt gaagagaatt tctaagtgtt taaaagggaa gaatttttgg atgcagtgta
     6841 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nattatatcg agacgcggtc
     6961 gtcgagtgat tgcgtagcaa tcgtatcgag acgaccgcct caatgttcat tgactgcctg
     7021 tagaagtgaa gaatcctaat gtatatatag ccagatcctt tggtgctggc gcacctcagg
     7081 ttgacagaaa tccaggtaaa cgacgagatt gcttcgtcct aacttcgtgg gactcgcaat
     7141 gacattggtt tacgtcactg agagtgaagc gaagcagtct caagtacccg aataagattg
     7201 cttcgccagc ggctcgcaat gacgatagtt acgttgcaaa atgcaatgtt attgcattca
     7261 ttatattgca ttttgcaata ttcattcaag gttatatttt tttattttcc gccataatgt
     7321 tgaggtatca ataccaagtg cggcagctgc atctttcttg ttagaatacc gtgctaaagc
     7381 ctgttttatc atggtttttt ccatctcttc aagcgagagt gttgaaacag gtgttatgcc
     7441 atatacatag gcaggcaggt gttcaagctt tatgtatgcc tcatgacata agacaaatgc
     7501 atgttcaatg atgttttcaa gttcacggat attgcctgga tagtcgtaat taagcaagat
     7561 attcattgcg tcatcggaca ctccttcaat gcttttccct tttaaggtat tgtatttttt
     7621 aataaagtgt tccaccagta atggtatatc ttcttttcgc tgtgataatg atggtaagcg
     7681 taatcctatg acattcaggc gatagtataa atcgtcgcga aaattaccag ccgcaacttc
     7741 ttctcgcaaa ttcttattgg tggcagcaat aatgcgtaca ttagcttttt ttggttttgt
     7801 gtcgccaaga ggttcatatt ctttgaattg caaaaatcga agtaatttca cctgcattga
     7861 taatggcagt tcgccaatct catccagaaa cagtgtgcca ccttcagcca tggctatttt
     7921 gccaggttta tctttttttg catctgtgaa cgcaccagtt ttgtatccaa acaattctga
     7981 ttcaatcaag ttttcaggta atgctccgca atttaccaca acaaatggct tctcttttcg
     8041 cctgcttaaa gaatgaatgg cacgtgcaat gagttctttc ccgcttccac tgggtccttc
     8101 aattaacaca gagctatcgc ttaaggctac atcaggcagt atggaaaaaa tctgctgcat
     8161 ctctttattt ttacttatga tgtcttcaaa agagtaggtg gattcaattt tctttctcaa
     8221 agtttcaata tcgctgatat cacgaaatgt ttccacgcca ccaatgatat ttccctgatc
     8281 atctttgagt aatgcggtac ttatgcttat ggttactttt tcacctgatt tgttaactat
     8341 atatactgtt ttattaataa tattttttcc tgtctgcatg gtttcacgaa gcgcacagga
     8401 atgctcacaa atatttgcat gaaagatttc aaagcaaagc ttaccaatag cttcgtcttt
     8461 actttcaaga cctaaaattt tacaggcagc attattcaat gacgtgattc tgaaatccat
     8521 atcaatggta aacacaccat cagcaatact gtctaaaata atgtttgcta cacaatcaga
     8581 gttcatcatt ctacattgtc tgtgtcattg tcaatgtttt catgttcatc attattatgg
     8641 ttattatcaa actgtgtgtc actattgtct tcatcattac tatcattata attgtttgta
     8701 gcatcatttt cattattttc ttgtttcttt aattcttcat gttttttttc ttcttctttc
     8761 cgcttttcaa gtaattttgt aagatagtta ctgatttcag aatcgttgat tgtactggca
     8821 catgatacaa tcacagcaga ttcattcata atgatgagat tcattggtat atcaatacaa
     8881 taaatattcc caagctgata tatcgaaagg ttaaattttt caggttgttc acggtaaact
     8941 atgatcaatg cctgtttagg tactgtatta ccaaatgcca gtctatattt ggatgctgcc
     9001 agtaatcctt cagctatttt ttgtgaatca aaaagattct gtgaagaacc taattcctga
     9061 tagttgtgat agataaaaat atattgtcca aatttatccc ggtagggtat aactggaaat
     9121 ttatccgttg cagaaatagt tgaatgtggt ttagctgtag tagtaatctc tacacctgca
     9181 gttaggtcat tctctatcca ttgataggta acctgtgtat ctttttcatc tggtaagttt
     9241 ccctttataa catcattatt aaacgttttc ttatctgttt gatctttgaa atagattgaa
     9301 gttactctaa attttataaa taatgacctg tctttttttg gttcaacgta atgaacaata
     9361 tcaaaatcaa aacagccgat agtcaatata catgaaataa ttgcaatgat gtaaaatttt
     9421 ttcataaaaa tttctccaaa tttttaagaa tgtacatcac caataaaact aaaagatatt
     9481 tattgataaa aaataagttt ctgtaatcgt aaagctctgt aatatataat tgatttattc
     9541 taacagtgtt aatatagtac atccataata catcaaatgt caaaacatat ttgaaatggc
     9601 ttagtcaaat gctcgcgtcg ttttcacgat tggagaaatt gtttgatggt taaaaaaatt
     9661 aaatgttcat tccagccatc tatggtagca tgcatgcata ttatttgttt ttttcatgga
     9721 atcgatttta tgaataaata gtataatagt ttcataaaaa agttgacatt tatgtataat
     9781 attactacaa tattatatgt taacataata ataaatattt cctttatctg ccaatctaat
     9841 tttctgctta tcgtgataac atactggatt attaaataat aagctttgtt gtattgatga
     9901 tataaataac aaaaaaaata aaattattag gaggtttacc atgatgaggt atccattact
     9961 gctcactaat tttatggaac gagcagcaaa atatttcccc aaaaaagaaa ttgtatcagt
    10021 atatcccaac gaaaagtttg tgtataatta tggtgaatgg tataaacgct gttgccagct
    10081 ggcacatgcg ttacaatcac ttaacattaa aaaaggtgac cgtgttgctt cctttgcatt
    10141 gaataaccac cgacatttag aattatattt tggtgtgcca tgtatgggtg cagtactcca
    10201 cactctcaat gttcgtttgt ctcatgagca tcttgtgtat attattaatc atgctgaaga
    10261 taaaatcata ttcgttgatg aagatctgta ttttctactt gagcctgtta aggataagtt
    10321 gaaaactgta aaacattatg taattttatc tcaatctggt aaaatgcctg aaacaaagtt
    10381 atcgcctgca ttgttatacg atgacctgat aaaggatttc cctggagaat ttgaatttcc
    10441 taaagataga gatgaatatg atcctgcact tatatgctat acatcggcta caaccggtga
    10501 tcctaaaggt gttgtttatt cacatcgggg ccttgttctt catgcgtatg cagcaggagt
    10561 tactttggga ttaaatgaaa gtgattgtgc aatgcatgtg gttcccatgt ttcatgctaa
    10621 tgcatggggt ggaccatttg gtgctacagg tttggggtgc aaacaggtac tacctggaag
    10681 acaaattctt gatatgaaag cattatgcaa aatcatttca gaagaaaagg ttacgtttac
    10741 ctgtggagtt ccaactatat ggatgatgct ccaccagtat ttagaaagtg gtggcgaaca
    10801 tgatttttca tcaatacgtg cggtgtattc tggtggtgca gcaatgccca ggtatttgat
    10861 ggaatcattt gccaaaaaat ataatttcaa tattgttcag gcctacggaa tgaccgaaac
    10921 atcaccactg gcaacagcag ctatccccaa aagttatatg caaaaccttt cagataagga
    10981 attatttgat ataaaaacca ctgcagggct tctggcattg ggtttagaaa tgaaggttgt
    11041 aaatttagaa acaggaaaag aagtccagtg gaacggcaaa gaaatggggg aagtttgttt
    11101 gcgtggtcca tggatagcca gtgaatatta taaagatcct gaaagaagta aacaaacatt
    11161 taaagatggt tggttgcata caggcgatat agctacaatc aatgaagagg gctatatttc
    11221 ccttgtagac cgtacgaaag atttaataaa aagtgggggt gagtggattt catctgttga
    11281 tctggaaaat cagattatgg ctcatcctaa agttcttgaa gcagcagtca ttgcaatgcc
    11341 tcatgagaaa tggcaggaaa ggccgcttgc atgcatagta ccaaaacctg aatttgcaga
    11401 ttcaataaca aaagaagaca tcatggaatt tctggaagca aaagttgcta agtggtggtt
    11461 gccggatgaa attgtatttt taaaggaaat acctaagaca agtgttggaa aatttaacaa
    11521 gaaggaattg aggaaaacag tattgcctga agtacttaaa aaatagtaga taatattaaa
    11581 aagttttgaa tagcatgcct atttctaatt ggatattata caggtaatca cgccaattag
    11641 tatcttgtat aacgttagaa aaataaaact ggccatgagg acctgcatat aaataagtaa
    11701 acttgttgaa tgcaaaagcc agtctaagtt gtaggccaca cccaaactgg ttgtaattta
    11761 taaatctgat accattggta ctgtcattat catttgacat ggtgatatga aaaatatgca
    11821 tatagtttag tccgtacaga atatctaaac gcaccttgtt cccaaatgac gatattaaat
    11881 ataaaggtat tataatggaa tgattttggg aggttatttg tgcatcaggt gtatcgccaa
    11941 attctaaata agtttcatga aatgcgtacg aaatcccaga gtgtattccg aaatgggagg
    12001 agaaaatgtt ttcaaaaaaa atacctcctc ctgcaattaa accgggtttc cattttacag
    12061 tttttccctc ttcactgcca cttctatgca atacatcatt cattccccca gcaacattga
    12121 ttccaaaact ataattccca ttgctggatt gtgcatggat agaagtataa agagaaaaat
    12181 aaaaaataaa taataatact aaataaagaa atttcttagc ataaagttgt atattaatag
    12241 ctaatatcat ttttaattta ccattattta ggcattatac atgttccatt aaatttagc
//
LOCUS       DAORYL010000090        12259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_4019_length_12259_cov_10.772124, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000090 DAORYL010000000
VERSION     DAORYL010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12259
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4019_length_12259_cov_10.772124"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..1186
                     /locus_tag="PLV81_13080"
     CDS             248..1186
                     /locus_tag="PLV81_13080"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HOM11071.1"
                     /translation="MKKFTISLPVLINFIFLISLVVSCTQQEKHVDIILSNILSSKTA
                     IEQYITKQSIDKSVHPIFVTFWDFDGTILKGDCSEGYKENDRQIYRGLVQIAIEQNFS
                     KLYKENEFDVFSNYYEHLDRTQGHLAAYTYLTTIFAGQNEDAIIKLSTQYFNETLQHY
                     YFSSSIAIMNELIKNNIHIYIISASPDFFVKGAAQSLKINPDAIHGIKLKNVNGILTN
                     TIVPPVTYAKGKTMKVLQIMKHLQQKYSTDHVYALAAFGNSYHTDGPFLEYVAKQKLP
                     AGTPVAVMINGGKEPDAYKGLFYCVKQSKIIQYSYK"
     gene            1205..2140
                     /locus_tag="PLV81_13085"
     CDS             1205..2140
                     /locus_tag="PLV81_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOM11072.1"
                     /translation="MKLFHFCIFIIFILTKPAYPEMWGYYLKDSLPYEKHQQYWENRF
                     ANLTVLCFTGITVSSTDCIIPEFTNEQALFKKAYERSIALIPHVTFTSVKSGIAFLTH
                     PQYWKKIIHTLAYAIRTSGWAGCHFDFEYLPSKYAANFAQFLKIFKAIAPDISLSAAM
                     FPQVEYNPTHAAFHDYALLSTYLDAIVLMCYDQHNPKTKPGPVTGVEWSRKNIEYALQ
                     FFKANQLWLGIPAYGYIWKDNTYYSVITMKSLSRYVTRYRNYRHTSGTIVIEYTQNND
                     HFIAYVPDVNLHNQLYELATSYKLKGIALWRLGFE"
     gene            complement(2201..3412)
                     /locus_tag="PLV81_13090"
     CDS             complement(2201..3412)
                     /locus_tag="PLV81_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11073.1"
                     /translation="MKFVKSMVLVAVLAVATAGFAQEAKPEEKVPDKPKLAVDGLAYL
                     EWSKELNHSKLGDPDENTNTFAIKRVYLNFKKKLDDIWSVRVTTDVGQVEAKSDKDYV
                     TQSGTPDTKSKVESKTNYYTVFLKYAYVEAKQKFDIADVKFQFGMIGTPLIGFVDKQS
                     DYRWLEQNYVDQAKTLLYYAATPADVANPGKWKTQSIDHSADMGLSLEVSMFNKLLTL
                     TGAYTNGEGYKYADEVKLGDDGKAYYGMATIMPVKDLYIFGIYRNQDTNDKIGDNYIR
                     QMGGGVVYSDGLFKIGAMYLLPEVSTKATGASAVENKYKLFDSWLNIRLDTIISMPVI
                     VVGRYAFGENKDLDKSKVTSWAAGLGYHVANGLRAVVYYQDTKADFKEKSAGFTGDSD
                     RKIFVKTEVKF"
     gene            4079..4870
                     /locus_tag="PLV81_13095"
                     /pseudo
     CDS             4079..4870
                     /locus_tag="PLV81_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
     gene            5113..6024
                     /locus_tag="PLV81_13100"
     CDS             5113..6024
                     /locus_tag="PLV81_13100"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HOM11074.1"
                     /translation="MKNENLTPQQYYVFRQCGTEPPFNNEYWDNKKHGIYVDIVTGAV
                     LFSSTNKFDSGTGWPSFTQPVQPDAVVERSDTSHGMTRTEVRSKSSDGHLGHVFDDGP
                     APTGLRYCINSASLRFIPVEKMIEEGYADYMYLFEGIQLPFEQIILGGGCFWGVEAYF
                     KKIAGVIDTVVGYSGGTTLNPDYKTVCSGTTGHAETVLVTFDPKIITLEKILKHFYAI
                     HNPSTPNRQGPDIGTQYRSAIFYVSDRQKHTIDEMVGKLKANGITVTTEVAKAKNFYK
                     AEEYHQDYLTKNPGGYCHINLNKIHEF"
     gene            complement(6029..6679)
                     /locus_tag="PLV81_13105"
     CDS             complement(6029..6679)
                     /locus_tag="PLV81_13105"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOM11075.1"
                     /translation="MGSYSLYDIPEADNGEVFQRFTEYFKNKDIQANASLSDKLKQFR
                     VQNKLSYEQLAAITGITSELLQKIEERQVYPSLKIIHQLTRSLQKAKAVAGSPVEWGY
                     TVIKKDEIIQSKNERPKERFHSGDHSHSAGTVNEAVAVKAYIITLEGNYTKKDFSIHN
                     GEEFIHVKKGAIKITLGANEVTLQQGDSIYFLSLIPHKIENIASHESIIHVIVYQE"
     gene            6888..7742
                     /locus_tag="PLV81_13110"
     CDS             6888..7742
                     /locus_tag="PLV81_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11076.1"
                     /translation="MERIVKSIIPNYNLHKIMGFSESIPIPNKDAAAQIVRDFSESRL
                     FFKLVHALMEAQEYGIMGRRYPIAHLHEIVGEIINLGFIYDKNNRIFIENSKTRKTPN
                     WGVLEEDEEYIVTLLRLDIAANTRLVRKYESPLITQTYNNLRSIVTAAVEQRNGRVWN
                     WEGDGCIAAFILGNRNMAAVCSAIEIFHELFLFNYIESQLPEPVNIRVAIDTGYCTYK
                     HSPEELKKTELVSKILELESRHTPLNSITITNSIYASLHAEIANKFTTTRSGNAVYYI
                     YTIQWATP"
     gene            7739..8698
                     /locus_tag="PLV81_13115"
     CDS             7739..8698
                     /locus_tag="PLV81_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11077.1"
                     /translation="MNIIIIYQKKNLPVLQKAFSTIIQSKKFSTAFIDSTESRKIQPP
                     DKNTIYYLDITGIKKAEIEKQVKKFNTNGHYFAIIDLKKSYTDIAWFFHNNACDYLTK
                     DMLVKPLSEKRMQSIMKLKGITPKTQHAHNYILAKDSWKGIVPGNEYTFCFLFIEILD
                     IGELKKHIGSEHLNQKIFSFRRYVEELLYQDDGRCWMWMDATGLFIFPFNGTSVPAIY
                     SAIRLIVNRLIISLEHTVFNQIIRYRIAMHIGNTVYQPRGKTGTIISDCINSLFHLGQ
                     KYTDEDGLFITEDVYSFLNEKTKKLFMKAKPFEGRQIYTFKYK"
     gene            complement(8700..9005)
                     /gene="yhbY"
                     /locus_tag="PLV81_13120"
     CDS             complement(8700..9005)
                     /gene="yhbY"
                     /locus_tag="PLV81_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011715238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HOM11078.1"
                     /translation="MNSIPRKKIKYLKACAHSMKPVVLIGKNGLSQAVLNAIDEVLLS
                     HELIKIKFIDCKEERRELAQQIVEKTGSSLVTMIGNVLIIYRQHPEAEKRQYELAAM"
     gene            9279..10043
                     /locus_tag="PLV81_13125"
     CDS             9279..10043
                     /locus_tag="PLV81_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014244941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOM11079.1"
                     /translation="MANILTDDPGYTYHEKTPKIDAKEFQKVIDSRRSVRIYEKDPIP
                     EKIIRQCLHNALLAPNSSNLQPWEIYHIVDPAIRQQLNEACLGQIAAKSAATLFVAVA
                     RTNTWRQHAQEMVRLFESAGNVPKAAISYYKKLVPFVYSQGFLGTWGIVKRLLFFIRG
                     LSAPVIREPVSKCHIKIWAVKSTALACQNLMLSLRAYGYDSCPMEGYDSKRIKKILNL
                     PKDAVVVMVISAGKRAVGGVYGPRIRFDENRFLKKV"
     gene            10057..10824
                     /locus_tag="PLV81_13130"
     CDS             10057..10824
                     /locus_tag="PLV81_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HOM11080.1"
                     /translation="MNIVREIVNDIIIITINRPEVRNAIDRPTAEELTEAFKNFDADD
                     NLKVAILTGADGNFCAGADLLAIAEDGHRKNKLNPNMKDDAPLGPTRMLLSKPVIAAI
                     SGYAVAGGLELACWCDIRMADSTAKFGVFCRRFGVPLIDGGTQRLPRLIGLSRALDLV
                     ITGREINAEEAYQFGLVNYLVKGDIKKEALALAQNIASFPQNCLRSDRMCVYKGFNMP
                     LPDAMEMEFARGTDVIRSGETLEGARKFAATKGKKLP"
     gene            complement(10885..11415)
                     /locus_tag="PLV81_13135"
     CDS             complement(10885..11415)
                     /locus_tag="PLV81_13135"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11081.1"
                     /translation="MKKISVCIAIAITGIVIVSCASTEKKVGCVEGKCNEGYGVYVWE
                     DGSKYAGSFKAGRFNGQGTFTWPDGRVYTGIYVDGKRQGYGVMSWPDGSVYKGQFAND
                     DITGNGTYKYADGKVYTGAMVKGKANGKGILEWPDGTRYEGEFIDNRMEGQGMLVYPD
                     GRTLKGRFVNGILVNE"
     gene            complement(11617..11886)
                     /locus_tag="PLV81_13140"
     CDS             complement(11617..11886)
                     /locus_tag="PLV81_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOM11082.1"
                     /translation="MKVTVEKINQEDLKKQGVFSWPIWEKEISDFDWYYDTTEQFYVL
                     EGEVEVETEDGQKVVFGPGDFVTFPKGVKCRWHVKKPIRKHYNFK"
BASE COUNT         3951 a         2467 c         2100 g         3741 t
ORIGIN      
        1 gctgaagaga aatacaggta gtagtttcaa aatacatcgt ttcctcccaa agtatatatt
       61 tttatcacgg tttattgtaa cgaaattata gtatcgataa ctatcgttgc aacaattttt
      121 tatatatatt aagaacaata ccaaaaaaga agttgaaaaa tggtaatgac ttattaaaat
      181 aaatgtttta ctgaataagt ataaaatatt tgtagttatt caaaaatact gattaagaaa
      241 atatactatg aaaaaattta ctatctcact acctgtactg attaacttta tctttctaat
      301 tagtttagtt gtatcatgca cacagcagga aaaacatgtt gatataatcc tttcaaatat
      361 cctttcatca aaaactgcta tcgaacagta tataacaaaa caatctatag ataaaagtgt
      421 tcatccaatt tttgttactt tctgggattt tgatggcaca attttaaagg gagactgttc
      481 tgaaggttat aaagaaaatg acaggcaaat ctacagggga ctggtacaga tagcaataga
      541 acaaaatttt tcaaagttat ataaagaaaa tgaatttgat gtgttttcca attactatga
      601 gcatcttgat agaacgcagg gtcaccttgc agcatataca tatctaacga caatctttgc
      661 aggacaaaat gaagatgcta tcattaaatt atccacacag tattttaatg aaacgttaca
      721 gcattactat ttttcttcat caatagcaat catgaacgag cttattaaaa ataatattca
      781 catctacatc atttcagcaa gtcctgattt ttttgttaaa ggtgctgcac agtcactaaa
      841 aataaatcct gatgccatac acggcatcaa attaaaaaac gttaatggca tactaacaaa
      901 caccatagtt cctcctgtta cctatgcaaa aggcaaaaca atgaaggttt tacaaattat
      961 gaagcattta cagcaaaaat actcaacaga tcatgtatat gcacttgcag cttttggtaa
     1021 cagctaccat accgatggac catttttaga gtatgttgcc aaacagaagt taccagcagg
     1081 cacgccagta gctgttatga ttaatggtgg caaagaacct gatgcatata aaggtctatt
     1141 ctattgtgtt aaacaaagta agattataca atactcatac aaataattat atacagcaca
     1201 atacatgaag ctatttcatt tttgcatctt tattattttc atactgacaa aacctgcata
     1261 ccctgaaatg tggggctact acctgaaaga ttcactgccc tatgaaaaac atcagcaata
     1321 ctgggaaaat cgttttgcca atcttactgt gctgtgtttt acgggaataa ccgtgagcag
     1381 tacagattgc attattcctg aatttactaa cgagcaagca ctttttaaaa aagcctatga
     1441 gcgcagcata gccctcatac cccatgtaac ctttaccagt gttaaaagtg gcattgcatt
     1501 tttaactcat ccacaatact ggaaaaaaat aatacacaca ctggcttatg caatacgtac
     1561 cagcggctgg gcaggatgtc attttgattt tgaatacctg ccatccaaat atgcggcaaa
     1621 ctttgcccaa tttttaaaaa tatttaaggc gatagctcct gacataagcc tatcggcagc
     1681 aatgttcccg caggttgaat ataatcccac ccatgctgca ttccacgatt atgcacttct
     1741 tagcacctat cttgatgcaa tcgttttgat gtgctacgac cagcacaatc caaaaacaaa
     1801 accagggcca gtaacaggtg ttgaatggtc caggaaaaat attgagtatg cgttacaatt
     1861 ttttaaagcc aaccaactat ggttaggcat tcctgcctat ggttacatat ggaaagataa
     1921 cacgtattac tccgttatca ccatgaagtc attgtccaga tatgtaacac gttaccgtaa
     1981 ctatcgccac acttcaggaa ccattgttat agaatatacg caaaataatg accattttat
     2041 tgcatatgtt cctgacgtaa atctgcataa ccagttatat gagctggcta caagttacaa
     2101 gctcaaaggt attgcactat ggcgattagg atttgaataa aaaaatgagg gaggcttgca
     2161 tgcttccccc attcttggga tagaattatg gttgatgttg ttagaattta acttctgttt
     2221 ttacaaaaat ctttctgtcg gaatcaccag taaaacctgc agacttttct ttaaaatcag
     2281 cttttgtatc ctggtaatat accacagctc gcagaccatt ggcaacatga tatcctaacc
     2341 ctgctgccca tgatgttacc ttagatttgt caaggtcctt gttttcgcca aaagcgtatc
     2401 taccaactac tataacaggc atgctgatga tagtgtctaa ccttatattg agccagctat
     2461 caaacaattt gtacttgttt tcaacagcgg aagcccctgt agcttttgta ctcacttcag
     2521 gaagcaggta catagcacct attttaaaaa gcccatcaga gtaaaccaca ccaccaccca
     2581 tctgtctgat gtagttatca ccaattttgt cattggtatc ctggttgcga tagatgccaa
     2641 aaatatacag atctttaact ggcataatcg ttgccatgcc atagtatgcc ttgccatcat
     2701 cacccagttt tacctcatca gcatatttat acccttcacc gttagtgtat gcaccagtga
     2761 gtgttaaaag cttattgaac atggaaacct ctaaactcaa acccatgtca gcagaatggt
     2821 caattgactg tgttttccat ttgcctggat tggctacatc tgcaggagtt gcagcatagt
     2881 aaagtagtgt ctttgcctgg tcaacatagt tttgttccag ccatcggtaa tcactctgct
     2941 tgtcaacaaa accaatgagt ggtgtaccaa tcataccaaa ctgaaactta acgtcagcaa
     3001 tgtcaaactt ttgttttgct tcaacataag cgtactttaa aaatacagtg taatagttag
     3061 tcttggattc aacttttgat tttgtatcag gagttcctga ttgagtcaca taatccttat
     3121 cacttttagc ctcaacctga ccaacatctg tggttacccg cacactccat atgtcatcaa
     3181 gcttcttttt gaaatttaaa taaacccgct taatggcaaa tgtgttggta ttttcatcgg
     3241 gatcaccaag ctttgaatga ttcaattcct ttgaccactc taaatatgcc agtccatcaa
     3301 ctgccaattt tggtttatcc ggtacctttt cctctggttt tgcttcctgt gcaaacccag
     3361 cagttgcaac tgcaagcact gctactaaca ccatactctt tacaaacttc attaaaacct
     3421 ccttaaacgt tagttaaatt attgatgtta tgatttttca aaatctgatt gtaaataagc
     3481 agcactatat aaagtaattt tgtggcaatt gttaagattt tataaatttt cttaattttg
     3541 tcttctatta tcttttactc atctgctctt acatatcatg catggtacat atttcatgtg
     3601 tgtttatttg atcatactaa gtacgctgaa caaagtatgg tacaaatatt tttgtttctt
     3661 ataatccacg tatcctacgg atatactgtg gtatatcttt tatataaaaa aattggattc
     3721 agtctaaaat atttttacaa tacatttcca cttagctgca taacaacgta atcctgtatt
     3781 gtgtattggg attaataatt atttccaaat aaagagtatt gacaaaatga tatatttttt
     3841 tacattttcc aatcgtgact caatactctc atttttaaca attaactatc tctcaataaa
     3901 ggtggtaaac gggaaggctt atgatgttta atccattaac aacacgatta tgtcagtgat
     3961 gtgcatataa ggggccaggt tggttccctt ttatccggtt taccattgcc tgcctgccat
     4021 aacttcataa agcttcctta aagttccggc acaggtggtg gccggtatat ttaactttat
     4081 tgatcttcct cagatgaaat ctgatgaagg caaaaaactc ctgaaacagg agtataaaaa
     4141 gtttatgaca aaacttgagc aaataagcgg taaaaaaatt acagcagatt cacttaaaaa
     4201 gggaattgaa attaccaacg ccaagcgtaa tgctgttaaa cgtttagcaa cacttcgaaa
     4261 agccaatccc gttcccatat caggacttga tgcattgctg gtgaatcagg tgttttttta
     4321 tgatgatcct gtacgattta ctgaatcagt caacaagcta tgtgatgaac tagaagagcg
     4381 aataaaaaat aaggtgggtg tattccctga acacacaaaa cgtcttgtca tttctggttg
     4441 tcccatggct gttccaaact ggaaggtacc atggattatt gaaacaagcg gtgcaactat
     4501 agttggcgaa gaatcctgtg ttggcgagcg tggtacacag tggattactg aagccagagg
     4561 caatactgtt gacgaattac ttgatgcaat tgttgagcgt tacttccaga tagattgtgc
     4621 aatctttaca ccaaatcctt cacggcttga acatatcaaa tccatggtaa aagagcttaa
     4681 agcagatggt attatccatt atggattaca gttctatcag ccatatatta ttgaaagcca
     4741 taccgtggaa aatagccttg aaaaggatgg cattccaaca ctgcgtattg aaactgatta
     4801 tggtcaggaa gatgccggac agcttaaaac ccgtattgaa gcatttgttg agatgctggg
     4861 taataaataa tataacaata cgcatagact caacattaaa atcaaaacgc gaaaaaactg
     4921 ctaaaagaag taatatttca aaatacaaac ctttgaccct ttaccgataa ctatcgcccc
     4981 atattatttg taataattac aatatgctat tgacttttga ataaaaatta ttatatttat
     5041 accaccatga aacagatttt catatttatt atacttatag taagctgtaa ctattattac
     5101 aaggaacaac ccatgaaaaa tgaaaattta acaccacaac aatattatgt attcaggcaa
     5161 tgcggcaccg agcccccgtt taacaatgaa tactgggaca acaaaaagca tggcatttac
     5221 gttgacattg ttacgggcgc ggtgcttttt tcctcaacca acaaatttga ctctggcacg
     5281 ggatggccaa gctttacaca gccagtacag cctgatgcag tagtggaacg ctctgacacc
     5341 tcacacggta tgacacgcac cgaagtgcgc agtaaaagct ctgatggaca ccttggtcat
     5401 gtatttgacg atggtcccgc accaacgggg ctgcgatact gcattaattc agcatcatta
     5461 cgctttattc cggttgaaaa gatgattgag gaaggctatg cagattacat gtatcttttt
     5521 gaaggtatac aattgccatt tgaacagatc atccttggtg gtggttgttt ctggggcgtt
     5581 gaggcatact tcaaaaaaat tgcaggggta attgacactg tcgttggcta cagcggtgga
     5641 accacactaa accctgatta taaaactgtg tgcagcggta caacaggaca cgctgaaaca
     5701 gtactggtaa catttgaccc taaaatcatt acgttagaaa aaattttaaa gcatttctat
     5761 gccattcaca atccctcaac accaaaccgt cagggaccgg atattggtac acagtaccgt
     5821 tcggcaatat tttatgtaag cgataggcag aagcatacaa ttgatgaaat ggtaggaaaa
     5881 ctaaaagcaa acggcatcac ggtgaccact gaagttgcaa aagccaaaaa tttctataaa
     5941 gctgaagaat accatcagga ctatcttaca aaaaatccag gtggatactg tcacataaat
     6001 ttaaataaaa tacatgagtt ctgagtttct attcctgata cactatgaca tggataattg
     6061 attcatgtga ggcaatattt tcaattttat gtgggataag cgatagaaaa tagatactgt
     6121 caccctgctg caaggtaact tcatttgcgc ccagtgttat cttaattgcg ccttttttca
     6181 cgtgaatgaa ttcttcacca ttatgaatgg aaaaatcttt cttagtataa ttgccctcta
     6241 aggtaatgat ataggccttt actgcaacag cttcatttac ggttccagca gaatgtgaat
     6301 gatcacctga gtggaagcgc tcttttggcc tttcattttt tgattgtatg atttcatctt
     6361 tttttataac tgtatatccc cactcaacgg gacttccggc tactgccttt gccttctgca
     6421 atgaacgggt aagttgatgg attattttta atgaaggata tacttggcgc tcttctatct
     6481 tttgcaatag ctcactggta ataccggtta tggcagctag ttgctcatag cttaatttat
     6541 tctgaactct gaactgcttt aacttatcgc tgagcgatgc attggcctgg atatctttat
     6601 ttttaaaata ttctgtaaac cgctggaata cttcgccatt gtctgcttcc ggaatatcat
     6661 ataatgaata cgaacccatg gtactttcct taaaatatat attccactca ttgcgacaaa
     6721 taaagtatat cctgaaaaat atcatctatc agataaaaaa ttattttaca agaaattttt
     6781 tttgacaaat ttgcgttaca ctatagtatc atcataccat gaaaattccc tattccattt
     6841 tatcgataac ggaaaaagct ctgaccaaat ccatggatgt ccataccatg gagcgtattg
     6901 tcaaaagtat catacccaat tataacctcc ataagataat gggcttttcc gaaagcatcc
     6961 ctatccccaa caaagatgca gctgcgcaga ttgtacggga tttcagcgaa tccaggcttt
     7021 tttttaaact ggtacatgct ctcatggaag cgcaggaata tggcataatg ggacggcgct
     7081 accctatagc tcatctgcat gaaatcgttg gtgaaattat aaacttaggg ttcatttatg
     7141 ataaaaataa ccgtattttt atcgaaaact ccaaaaccag aaaaacccct aactggggtg
     7201 tccttgaaga agatgaagaa tatattgtca cattgcttcg acttgacatt gcagcaaata
     7261 ccaggcttgt ccgtaaatat gaatcgccat taatcaccca aacatataat aatttacggt
     7321 cgatagttac tgcagcagtt gaacaaagaa atggtcgtgt atggaactgg gaaggtgatg
     7381 gatgcatagc cgcttttatt ctgggcaaca ggaatatggc agcagtatgt tcagctatag
     7441 agattttcca tgagttattt ttatttaatt atattgaatc acaactgcct gaacctgtta
     7501 atatacgggt agctattgat acaggatatt gcacctacaa acattcacct gaagaactga
     7561 aaaaaacaga actggtgtca aagatactgg agcttgaatc cagacacact ccccttaact
     7621 caataactat caccaattca atttatgctt ccctgcatgc tgaaattgca aacaagttta
     7681 ccaccaccag aagtggcaat gcagtgtatt atatttatac aatacagtgg gctactccat
     7741 gaatataata attatatacc agaaaaaaaa cctgcctgtt ttacagaagg cattttccac
     7801 aattatacaa tcaaaaaaat tttcaactgc ctttattgac tctaccgaat ccagaaaaat
     7861 acagccaccg gataaaaaca ccatttacta cctggatatc accggcataa aaaaagctga
     7921 gattgaaaag caggttaaaa agtttaatac caatggccac tactttgcaa taattgacct
     7981 taaaaaaagt tatactgata tcgcgtggtt ttttcataat aatgcctgtg actatcttac
     8041 taaagatatg cttgtcaaac cgctgtccga aaaacgcatg caatccataa tgaaattaaa
     8101 aggaattacc ccaaaaacac aacatgcgca taattatata ctagcaaaag attcctggaa
     8161 gggaattgta cctggcaatg aatatacatt ttgctttttg tttattgaaa tactggatat
     8221 tggcgaatta aaaaaacaca ttggttcaga acatttaaat caaaaaattt tttcatttcg
     8281 caggtatgtt gaggaactgc tttatcagga cgatgggcgt tgctggatgt ggatggatgc
     8341 aaccgggctg ttcattttcc cctttaatgg cacatctgtt cctgcaattt actctgcaat
     8401 tcgccttata gtaaaccggc taattatttc actggaacat accgtgttca atcaaatcat
     8461 acgctatcgt atagccatgc atattggtaa cacggtatat cagccgcgcg gcaaaaccgg
     8521 taccattata tccgattgta ttaattcatt atttcactta ggtcaaaaat atacagacga
     8581 ggatggatta tttatcaccg aagatgtata tagcttcctt aatgaaaaga caaaaaaact
     8641 atttatgaag gcaaaaccat ttgaaggcag acaaatttac acattcaaat acaaataaat
     8701 tacatagctg caagttcata ctgccgcttt tcagcttcag gatgctgccg gtagattatt
     8761 aacacgttac caatcattgt gacaagactg cttcctgtct tttcaactat ctgctgtgcc
     8821 agttcccggc gttcttcctt gcagtcaata aatttgattt tgataagctc gtgactgagc
     8881 aacacttcgt caatggcatt aagtacagcc tgagacagcc catttttgcc tattaacaca
     8941 actggcttca tgctatgggc acaggctttt aaatatttaa tcttcttgcg gggaatgcta
     9001 ttcatacaat cctcaataaa ttaaaaatat taatattgca caatccattc acaccgcatt
     9061 tattttttta taattgacat taatatataa ttatgatagc ctttttgcaa ataaaattaa
     9121 ttatcatttt attgcaataa ttttttatta atttattttt acatcatgct tgggcataag
     9181 tccatgtatc aaaatgcacc acaataaatg atgtatgttg ttgtacctgg acattgaagt
     9241 acacaagctg atgaacatat tttcaggagg aaagacaaat ggctaacatt ttaactgatg
     9301 atcctggtta tacatatcac gaaaagacac caaagattga tgcaaaagaa tttcaaaaag
     9361 taatagattc acgaaggtca gtacgtattt atgaaaagga tccaataccg gaaaaaatta
     9421 tacggcagtg tttgcacaac gctttgctgg cacccaattc atcaaacctt cagccatggg
     9481 aaatctatca tatcgttgat cctgccatac gccagcaact caatgaagcc tgcctggggc
     9541 agatcgctgc caaatctgct gctacattat ttgtagctgt agcacgaaca aatacatggc
     9601 gtcagcatgc acaggaaatg gtaagacttt ttgaatcagc tggcaacgtt ccaaaggctg
     9661 ctatctcata ttataaaaaa cttgttcctt ttgtatacag ccagggattt ttaggcacat
     9721 gggggatagt aaaacgcttg ctattcttta tacgcggact ttcagcacca gtcatacgtg
     9781 aaccagtatc aaaatgtcat atcaaaatat gggctgttaa atccaccgca cttgcatgcc
     9841 agaatctgat gctttctctc agagcgtatg gatatgactc atgccccatg gaaggctatg
     9901 actcaaaacg cataaaaaag attttaaatc ttcccaaaga tgccgtagtt gtcatggtga
     9961 tttctgcagg taagcgtgcg gttggcggtg tctatgggcc acgcattcgc tttgatgaaa
    10021 acagattttt gaaaaaagta taatagggtt acatgtatga atatagtacg ggagatagta
    10081 aacgatataa tcatcataac cattaacaga cctgaagtaa gaaatgccat tgaccgccct
    10141 actgcggaag aattaactga agcatttaag aattttgacg ctgacgataa cctgaaggtg
    10201 gcaatactta ccggtgcaga tggcaatttt tgtgctggag ctgatttgct tgccattgcc
    10261 gaggatggtc acaggaaaaa taagcttaat cctaacatga aggatgacgc cccgcttggc
    10321 cccacacgaa tgcttctatc aaagccggta attgctgcca tatccggata tgctgttgcc
    10381 ggtgggcttg aacttgcctg ctggtgtgat atacgtatgg ccgattccac agctaagttt
    10441 ggggtatttt gccgcaggtt tggagttccc ttaattgatg gcggcacaca acgtttacca
    10501 cggcttatag gcttaagcag ggcactggat cttgtaataa ccggcagaga aattaatgct
    10561 gaagaagcat atcaatttgg cctggtaaat taccttgtca aaggcgatat aaaaaaagaa
    10621 gcgttagcat tagcacaaaa cattgcatca tttccacaaa attgtttgcg cagtgacagg
    10681 atgtgcgtat acaaaggatt taatatgcct ttacctgatg caatggaaat ggaatttgcc
    10741 cgaggcaccg atgtcattcg cagtggtgaa acgctggagg gtgcacgaaa atttgcagct
    10801 actaaaggca aaaaacttcc atagtatgta tatacatttt gataaataat caataatact
    10861 attcagtata gtgaaataat taccttattc attaaccagt atgccattta caaaacgccc
    10921 ttttaacgtt cggccatcag ggtataccag cattccctgc ccttccattc tgttatcaat
    10981 aaactcacct tcatagcgtg taccatcggg ccattcaaga attcctttgc catttgcttt
    11041 cccttttacc attgctccgg tatatacttt tccatcagca tatttgtacg taccattacc
    11101 ggtaatatca tcattagcaa attggccttt gtatacactg ccatcaggcc aggacattac
    11161 tccatatccc tgtcgtttgc cgtcaacata aattcctgta tacacacgac catctggcca
    11221 ggtaaacgtc ccttgcccat tgaatctccc agccttaaaa ctgccagcat atttagaacc
    11281 atcctcccat acataaacgc catagccttc attgcatttc ccttcaacac agcctacctt
    11341 tttttctgta gaagcacatg atacaataac aataccagta atggcgatag ctatacaaac
    11401 actgattttt ttcattatta attatcctcc tttcaacata gtatgaagaa caaaacactt
    11461 ttctgagtag aaattccaca aaaggttgct aagccctttg atgaactcag taatcgcctg
    11521 tcgaattgct cagaagatgc gctttgctca cagtgactta aagaaagtca ttacaatttc
    11581 ccttcagcgc tctcaataac acttaatatt cattcatcac ttaaaattat aatgcttccg
    11641 tataggcttt tttacatgcc agcggcattt cacgcccttt gggaatgtaa caaaatcacc
    11701 agggccaaaa acaactttct gaccatcctc ggtttcaacc tcaacctcgc cttccagtac
    11761 ataaaactgt tcggtagtat cataatacca gtcaaaatct gaaatttctt tttcccagat
    11821 tggccatgaa aatacacctt gcttctttaa atcttcctga ttaatttttt caacagtaac
    11881 tttcatgtat ggtatccctc ctttcgtcgg ataatagact ttattaccat cattcacatc
    11941 acattctttg ataaaatcag taacattatt acaaagctca ggcggcaaca tcattcccat
    12001 gaaaaattct tgaacaacta ttacctccac attgtaaacg taagtactac ccttttgtgt
    12061 aatatttgcc tctacacata cagtagcacc ccattataaa caactttatc cctttgtagt
    12121 tttctataaa taatgctggc agtcttttga aaatactata atcaatgtag catgtgattt
    12181 tcaaccaaaa tattcaatac atcaataaat tatcgcttta tagtatgctc tgaccctttc
    12241 attgtttcta aaccgataa
//
LOCUS       DAORYL010000091        11978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_4115_length_11978_cov_13.364589, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000091 DAORYL010000000
VERSION     DAORYL010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11978
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4115_length_11978_cov_13.364589"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(351..827)
                     /locus_tag="PLV81_13145"
     CDS             complement(351..827)
                     /locus_tag="PLV81_13145"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOM11083.1"
                     /translation="MTLFKNKYRSETTRLKGFDYASPGAYFITICTYNREHVFGKIIN
                     GTMMVNETGKIVNECLHDLPNHYPNLKIDEYVIMPDHIHCIMVITEKYTHGIFEFVRA
                     LKSFSSRRINEMRKNKFPPIWQSRFYDHIIRNEYELNIIRNYIINNPKKWNNGRNK"
     gene            1037..1489
                     /locus_tag="PLV81_13150"
     CDS             1037..1489
                     /locus_tag="PLV81_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOM11084.1"
                     /translation="MKWGLTKRNETKLNPFEAFERDVKRIFDDFFTLEPVDLFDSAWV
                     PSIDVEEDEKSIHVRAEIPGIDEKDLNVTLEDNVLTISGEKKEERKEENKRYVLAERR
                     FGSFKRSIALPAEVKADNVKASFKNGVLTVDFEKKEVSQPKRITINVK"
     gene            1503..1913
                     /locus_tag="PLV81_13155"
     CDS             1503..1913
                     /locus_tag="PLV81_13155"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOM11085.1"
                     /translation="MFTTYDLLDDVLTLRDMVDRFFDEVSTTRRRVEFPYINVYEKDD
                     DIVIRAIAPGVSSSDVNVQLADNTLTIEMNRKEDYADKPYLRKEREFGSYKKSIKLPY
                     YVDPDRVEATMNNGILTIKLTKHESVKPRKITIN"
     gene            1929..2309
                     /locus_tag="PLV81_13160"
     CDS             1929..2309
                     /locus_tag="PLV81_13160"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOM11086.1"
                     /translation="MATQVAKRTDRNLDKKDYCLVPPVDIYETDNEYVLKADMPGVPK
                     ENIEITFNNNELEINGKIDEAYTSSDNLTYREFTLCNYNRKFLVSDKINVEGITATLE
                     NGVLTVTLPKREEAKPKKIEIKAE"
     gene            complement(2348..2758)
                     /locus_tag="PLV81_13165"
     CDS             complement(2348..2758)
                     /locus_tag="PLV81_13165"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HOM11087.1"
                     /translation="MKSYILRILIVCMVLSTGIGCSKLMNNTCTTVDAQKYAGQEIVI
                     KNDGCITIEIESQLSTGYRWRFAIKENPGMLINSDYKVQTCEGQTVGGIDKEIFTFKA
                     VKAGKATVVFDYVRPFDKNPKPVKTKEFTITITE"
     gene            complement(2779..3933)
                     /locus_tag="PLV81_13170"
     CDS             complement(2779..3933)
                     /locus_tag="PLV81_13170"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOM11088.1"
                     /translation="MKKHILMSIIVCLVMLPFAFPQSTNKFKADVESQRIKNKKSIAE
                     VKQQLEAIRKDIKERNLKFKVDLTEALKRQIDEITGTVPPKDLEKEAEIQTSRGFQLF
                     LEFLQRLAEAQAAKRKAELQPEKKEPVKPKKEEAKKEEKTESVTPSKPEETLPQSFML
                     ADPSKPVLDWFALGMMTAVKDQGLCGSCWSFTAMGVVEGCYKINKKTDINLSEQYMID
                     CARDKYGRDAGSCNGGWYGNVFDFMMTNYPVEEKIAPYRGYEYGCTPKKQIPIKLVAW
                     GYVRKDGGIPSVDEMKKALCTYGPIAATVKVTPAFQAYVGGIFDEFTPVSSPKDVNHG
                     IIIVGWDDSKKAYRIKNSWGERWGEKGYMWIEYGCNNIGYGAAWVVLDKE"
     gene            4360..5199
                     /locus_tag="PLV81_13175"
     CDS             4360..5199
                     /locus_tag="PLV81_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11089.1"
                     /translation="MNSTRFLLYTIVAVLALSLSVTAIAQQQPPKEEQKKEGIKEEVT
                     GWRIKDFKPYVKAIQELEKLSKEYSDNLLKLAIDEYSTGIDILEDMENDVNKLIETNQ
                     KNKNLQEQWYWQEIDRREQEKRQIAMMKFEAKTKAVTYFTRAINHMDEITFEEVRKDP
                     KFVNFQIRLYQAYVSTQYDLYNLKPCIPVLERYIEINDQTKNDAWAYKYLSSCYGYME
                     GVLLKYKQGTEQEQLQYKQKKNMYMLRAAELQYGIDSPQYKHLREIVSKDEKKSEKIN
                     EFK"
     gene            complement(5273..6274)
                     /locus_tag="PLV81_13180"
     CDS             complement(5273..6274)
                     /locus_tag="PLV81_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11090.1"
                     /translation="MISKTGYHVYRIAFFSILFFLSFHFSFYSLAIGTAFPTLDNGTL
                     YTEPSVTVPVQGVVLDYAVRDTAILFVAKNEKEIVAGAYTIQDKNITQFPVTTHSENT
                     QIKRIAFESGAIYICIHESDSVLYRADVNTGMVEQVKGVSDFLILKDSLVILKSDNTL
                     VYNDFVLPLYFTGKPLLKGHVDDRIVFVTDDSDTEVIDIKEKKVVYRYSKNVQYVVSS
                     DYTVEVAINDLPTGEKSPRIFYKIYCNGQDYGRTEPVLAINQSKIQLNLAPEQYHEIV
                     IERWRLDENSEQYIRDNNIRQPKPVIVFVYPGRIIVVRLTYTGLEYNVFTGFKVMER"
     gene            complement(6243..7952)
                     /locus_tag="PLV81_13185"
     CDS             complement(6243..7952)
                     /locus_tag="PLV81_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HOM11091.1"
                     /translation="MRLAEPTIDHTPGKIPTDLEHLRKLFIMPDSPDKFMQFGYELLD
                     LIHSFFKEKGGIHSEISLPELACLFNNIDIPRDPKLLKDILLEIKQKVIKHSVKVGNP
                     YYIGHMTSAIPYFMILLEMIIAALNQNQVKIETAKASTFVEREFISWIHRLIFQKPAS
                     FYKRNIQNHRIALGNVTLDGTLANLTAMLVARNKAFPADEHFPGIRKAGVAEAFRYYN
                     IHRAVFIVSQRGHYSIDKVARITGIGDNNVIKIPVDSNNKIDINALRRELKAIAEYNS
                     THADTIKVIALIGIAGTTETGNIDDLYTLRKIADEYNIFFHVDAAWGGSVLLVNKYRD
                     LFKGIEKADSVTFDAHKLLYSPLSMGLILFKNPYDLTNIMHTSNYIIRPDSVDQGRFT
                     VEGSRPFSCLKPWVTLKIFGTSGFEILFDHAFMLTDTLKDLLIKHENFELLNNPELFI
                     TNYRFVPESVQTKIEHIYQRLQNANPQDTIRLKKRLAKINDVLNEMTIELHRAIRQED
                     NSFVSRTMLESTRYRDQRIVVLRAITINPLTTPAILQEIIDEQNKLGLKIYNDFKNRL
                     SRV"
     gene            8426..9157
                     /locus_tag="PLV81_13190"
     CDS             8426..9157
                     /locus_tag="PLV81_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOM11092.1"
                     /translation="MTILKLENVSLSLDEKPILNNLSVDFWEGHIHAIVGPNGAGKST
                     FSYVVMGISGYRNITGTIYFKGQAINNLTVYERARLGISLGWQEPARFEGLTVAQYLK
                     ASKKDKGMSIEEAMDVVGLSPQEYLQRACDKTLSGGERKRIELASLLVMQPELVLLDE
                     PDSGIDVEALERIFDAINILKERGSTVITITHSMAVLKKAEHAFLMCHGNIIDKGSID
                     KISGYFENKCIPCDHKNIPVLGEVQ"
     gene            9157..10095
                     /locus_tag="PLV81_13195"
     CDS             9157..10095
                     /locus_tag="PLV81_13195"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOM11093.1"
                     /translation="MDDKREIAKTLYKTMGLTDAALNDPDIAHLVINGNMVVGAHLVP
                     GLVVEPHETPNGVTVTMKVLAGSIIQKPVHLCFGMLPEDGLQEIIMDVTIEEHAKVSI
                     LAHCTFPNAKNITHKMQANIAIKEGAYYSYVERHVHAPGGLITVVPHAIVTVEKNATF
                     KTDFELLRGKAGIININYETTCYEGSLLEMNAKVNGIDDDDITIRETGHLKGQGAVGV
                     LTTRIAVRDNAKATVYNTLTAHAAFARGHVDCKEIIQDNGVAKAIPIVEVYHPKAHVT
                     HEAAIGSVDDKQLQTLMARGLDEDEAVDLIIQGLLS"
     gene            complement(10099..10530)
                     /locus_tag="PLV81_13200"
     CDS             complement(10099..10530)
                     /locus_tag="PLV81_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11094.1"
                     /translation="MKFDDSKIYFYFSIAVLVAGILFGLPGIYSKMVTEPAIEKLLAQ
                     NTDSQKLKQAYIMLRNPHIFAGYDRYDEAGAGIEYILKEFDNRVAQQKEFTSNDAMYL
                     ELLLVRRQQGSDLSIKTMIYFILLSLLGVTALAIEKHVNKK"
     gene            complement(10527..10988)
                     /locus_tag="PLV81_13205"
     CDS             complement(10527..10988)
                     /locus_tag="PLV81_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11095.1"
                     /translation="MIKYIIPLLLVVCAGCTQYVDVGDVEFSVQVVFQQNVPVFATTY
                     TITLLNTSINKVVKNYTATVAIVTEDKHTLKQLTFATDKLLPKQVQQITITQNESEEA
                     IKPLFNELEIDINEVIKQQEAALVLPEGMVKVEKAHYETVDIVTYLQEVGQ"
     gene            complement(11138..11857)
                     /locus_tag="PLV81_13210"
     CDS             complement(11138..11857)
                     /locus_tag="PLV81_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11096.1"
                     /translation="MYAIHRFLQETMKQKKISKKELAQRLGYNNISKGIRRIDEFLEK
                     ATLNEYIVYNLHSALDEPKEKILEKLEETKEEIVREIQKQEEEKRQKQEEERKNFKPF
                     LYCVTEYSKPSPIFICAMTQSFLLKKVDLPSTFNELSIEAKQKAIKDAIKEHHNFVGQ
                     KWHGRIPAYGKILAYTLVVEYDYNEEDLPVYAIDGSIIENPPRFYQDIELGVAYIGIE
                     KKKDITRFFKNVEIIVEKIQR"
BASE COUNT         3705 a         2315 c         2103 g         3855 t
ORIGIN      
        1 gacgggtgta gggacgggtg tagggacggg tgtagggacg ggtgtaggga cgggtctcag
       61 acccgtccct acacccgtat atcccgtccc tacacccgta taccccatcc atattcccat
      121 atatcccgac catacccata tatcccatcc atacacccgt ataccccgtc catattcccg
      181 ttaatcccga ccatacccgt atatccatcc atacatccat ataccccatc catacacccg
      241 tatatcccgt ccctacaccc gtataccccg tccatattcc cgttaatccc gaccatatcc
      301 gtatatccca tcattacacc cgttaattcc acccctgcaa cgcctatgta ttatttattt
      361 cttccattat tccatttttt ggggttattt attatataat tgcgtattat atttaattca
      421 tattcatttc ttattatgtg atcatagaaa cgtgattgcc atataggagg gaatttgttt
      481 ttcctcattt cattgattct acgtgatgaa aatgatttta acgcacgcac aaattcaaaa
      541 atgccatgtg tatatttttc cgttatcacc attatacaat gaatatgatc aggcattata
      601 acatattcat cgatctttaa atttggataa tggttgggca aatcatgtaa acactcattt
      661 acaatttttc ctgtttcatt taccatcata gtcccgttga ttatttttcc aaatacatgt
      721 tcacggttat atgtacatat tgtaataaaa tatgcacctg gcgatgcata atcaaatcct
      781 tttaaccttg ttgtttcact gcggtattta tttttaaaca atgtcatgat gttatccccc
      841 ttgttttcct gtaaatagaa aacgctggaa atcttaaaaa tttaaaaaaa tttcacaatt
      901 ttcagtgtct ttctaaaaat ttttctcctg ccccagttga cgattttaaa atctgttatt
      961 aatttattat cagaaattag cactcattaa tatagagtgc taacaataac aaccttatat
     1021 tcataggagg tgcactatga aatgggggtt aacaaaacgg aatgaaacca aattaaatcc
     1081 ttttgaagct tttgaacgcg atgtaaaaag gatttttgat gatttcttta cactggaacc
     1141 ggtagatctt tttgattctg catgggtacc ttcaattgat gttgaagaag atgaaaaatc
     1201 cattcatgta cgggctgaaa tccccggaat tgatgaaaag gacctcaatg ttactcttga
     1261 agataatgta ctgaccatca gtggcgaaaa gaaagaagaa cgcaaagaag aaaacaaacg
     1321 gtatgtactt gcagaacgcc gatttggttc attcaaacgt agcattgcat tgcctgctga
     1381 agtaaaggct gataatgtta aagcctcctt caaaaatggt gttttgactg ttgactttga
     1441 aaagaaggaa gtttcacagc ccaaacgaat aactatcaac gtaaaataac aggaggtgga
     1501 ctatgttcac aacatatgat cttcttgatg acgttttaac actcagggac atggtggacc
     1561 gcttctttga tgaagtatcc accacccgtc gacgcgtgga attcccttat atcaatgtct
     1621 atgaaaaaga tgatgatatc gttatacggg cgatagctcc gggagtttca tccagcgatg
     1681 tcaatgtgca actggctgat aatacattaa ctatcgaaat gaaccgtaag gaagattatg
     1741 ctgacaaacc gtatttgcgc aaagaacgtg agtttggcag ctacaagaaa tccatcaagt
     1801 tgccatacta tgtggacccc gacagggttg aagcaacaat gaacaatggt attttaacca
     1861 ttaagctgac caaacatgag tcagtgaaac cccgcaagat aaccattaac taaaaggagg
     1921 tgatagttat ggcaacacag gtagcaaaac gcactgatag aaatcttgat aagaaggatt
     1981 attgcctggt acctccggtt gatatctatg aaaccgacaa tgaatatgtc ctgaaagcag
     2041 atatgcccgg agttccaaag gaaaatattg aaataacctt caataacaat gaactggaaa
     2101 tcaacggtaa gattgatgaa gcttatacct caagtgacaa tctaacatac cgtgaattta
     2161 cactgtgcaa ttacaaccgt aaattcctgg tttctgacaa gattaatgtt gaaggcatta
     2221 ctgctacact tgaaaacggt gtactcactg ttacacttcc caaacgtgaa gaagcaaaac
     2281 caaagaagat agaaataaaa gctgaataat atcatgcccc atcgttaaca gaagatgggg
     2341 cattttttta ttctgtaatt gttatagtga attcttttgt ttttacaggt tttggattct
     2401 tatcaaacgg acgcacataa tcaaatacaa cagtagcttt gccggcctta accgccttaa
     2461 atgtaaatat ttctttatct attccaccaa cggtttgccc ctcacatgtc tgcactttat
     2521 aatcactgtt aataagcatt ccgggatttt ccttaatagc aaagcgccat cggtatcctg
     2581 tactcaattg tgattcaatt tcaattgtta tacagccatc atttttaata acaatttcct
     2641 gtcctgcata cttctgtgca tctaccgttg tacatgtatt attcataagt tttgaacaac
     2701 ctattcctgt agatagtacc atacatacta tcaggattct taatatatat gatttcataa
     2761 aacctccatg aattttcatt attctttgtc aaggacaacc cacgctgccc cataaccaat
     2821 gttattgcat ccatattcta tccacatgta gcctttttcg ccccaacgct caccccagga
     2881 gttctttatc ctgtaagctt ttttactatc atcccaacct acaataatta ttccatgatt
     2941 gacatctttg gggctgctca ccggtgtaaa ttcatcaaat ataccaccaa catatgcctg
     3001 aaaagccggg gtaaccttta cagtggcagc aattggtcca taggtacata atgccttttt
     3061 catttcatca actgaaggga tacctccatc tttgcggaca tacccccatg ccacaagctt
     3121 tatgggtatc tgttttttag gagtgcatcc atattcatat ccccgatatg gggcaatctt
     3181 ttcttcaaca gggtagttgg tcatcataaa atcaaataca ttgccatacc atccaccatt
     3241 acaactgcct gcatcccgtc catatttatc acgggcacaa tcaatcatat attgttctga
     3301 aagattaata tcggtttttt tatttatctt ataacaacct tccaccacac ccattgcggt
     3361 aaatgaccag cagcttccac acagtccctg atctttaaca gccgtcatca ttcccagcgc
     3421 aaaccagtca agcactggtt tagatggatc agctaacata aatgattggg gtaacgtttc
     3481 ttcaggtttg gatggcgtta cactttcagt cttttcctct ttttttgctt cttctttttt
     3541 aggttttacg ggctcttttt tctcaggctg caactcagcc ttgcgttttg cagcctgggc
     3601 ttcggccaat cgttgcaaaa attccagaaa taactggaat cctctgcttg tctgaatctc
     3661 agcttctttt tccagatctt ttggtggtac cgttcccgta atttcatcaa tctgtctttt
     3721 taaagcctcg gtcaaatcta ccttaaattt gagattacgc tcttttatat ctttccgtat
     3781 tgcttccagt tgctgtttta cttcagcaat actcttttta ttttttattc tttgtgattc
     3841 aacatctgct ttaaatttgt ttgttgattg gggaaaagca aaaggcaaca taaccagaca
     3901 tactattata ctcatcaata tgtgtttttt cataaacctt ccctccatat tttaacaatg
     3961 ggttaaaagt tcagaattct ttagcttcca tacatgattt gatataaaat aataaaaaac
     4021 tattcagttg cccactataa atctttcaat gcagcaaact aaataaatta tgaaatattg
     4081 taagtggtca tttttcgcaa aattttttaa attaatatta ctatcactca tacattattt
     4141 agtaacaatt gtccaacatt tttttactat ttcaatggtt atagcaatgc aaccaattgg
     4201 ctccatgtga acatgtataa aaaaattaca ttgctaaaat atcaacaaac cgttattatg
     4261 tgaaaaatat ttaatttttg tttttgtata accgataaaa aagtgtaata gcataattac
     4321 caattataga gtgtatatta tcataagagg gtattcacta tgaattcaac aagattttta
     4381 ttgtacacta ttgttgccgt attagcactt tctttaagtg tgactgccat agcacaacag
     4441 caaccaccaa aggaagaaca aaagaaagaa ggcataaaag aagaagttac cggctggcga
     4501 ataaaagact tcaagcccta tgttaaagca atacaggaac tggaaaaact cagcaaagaa
     4561 tattcagaca atcttttaaa actggcaatt gatgaatatt caactggtat agatatactt
     4621 gaagatatgg aaaatgatgt caataagctc attgaaacca accagaaaaa caaaaacctt
     4681 caggaacagt ggtactggca ggaaattgac cgccgtgaac aggaaaaacg ccagattgcc
     4741 atgatgaaat ttgaagcaaa aacaaaagca gtgacatatt ttaccagagc tatcaatcat
     4801 atggatgaaa taacctttga agaagtacga aaagacccca agtttgtcaa cttccagata
     4861 cgattgtacc aggcatatgt ttcaactcag tacgacctgt ataacttgaa accctgtatt
     4921 ccggttctgg aacgatatat tgaaattaat gatcagacca aaaacgatgc atgggcatat
     4981 aagtacctgt caagctgtta tgggtatatg gaaggtgtgt tgcttaaata caagcaggga
     5041 acagaacagg aacagttgca gtacaagcag aaaaagaaca tgtatatgct gagagcagcc
     5101 gaactgcagt atggtattga ttcacctcag tataaacatt tgcgcgaaat agtatcaaaa
     5161 gacgaaaaga agagtgaaaa aataaacgag ttcaaataag aatattacgt tcacgctact
     5221 ttaagcagga attgttcact atcaataaaa aaccccgtta cagtacgggg ttttatcttt
     5281 ccatcacctt aaaacctgta aacacattgt attccaatcc agtataggtc agtctgacta
     5341 cgatgattcg gccagggtat acaaatacta taactggttt tggctgccga atattattat
     5401 cccgtatata ctgttctgaa ttttcatcaa gacgccagcg ttcaatcacg atttcatgat
     5461 actgctcagg tgcaaggtta agctgaatct tagattgatt aattgcaagt acaggctctg
     5521 tacgcccata atcctggcca ttacagtata ttttataaaa aatcctgggg gatttttcac
     5581 cggttggaag atcattgata gcaacctcaa cagtataatc gctgcttaca acatattgta
     5641 cgtttttact atagcgatat actactttct tttctttaat atcaataact tcagtatcac
     5701 tgtcatcggt tacaaagact atgcgatcat ccacatgccc tttcaataac ggcttgcctg
     5761 taaaatataa tggcaataca aaatcattgt acacaagggt attatcgctt ttaaggataa
     5821 ccaggctatc tttcaatatt aaaaaatcgc taaccccctt aacctgctcc accatgcctg
     5881 tattcacatc agccctgtat aatacactat cactttcatg aatacagata tatatggcac
     5941 ctgattcaaa agctatgcgt ttgatttgtg tgttttcaga atgcgttgta acaggaaatt
     6001 gagtaatgtt tttatcctgt atagtataag ctcccgcaac tatttctttt tcattttttg
     6061 caacaaacag aattgcagta tccctgacgg cataatcaag aacaacgcct tgaacaggca
     6121 cggtcactga aggctcagta tataatgttc cattatctaa tgttggaaat gcagtgccta
     6181 tcgccaggct ataaaaagaa aaatgaaatg acaaaaagaa tagtatacta aagaaggcaa
     6241 tcctatacac gtgataaccg gtttttgaaa tcattatata tctttaaacc aagcttattc
     6301 tgttcgtcaa ttatttcttg caagattgcc ggagttgtta aaggatttat ggtgatagcc
     6361 ctgagaacta caattcgctg gtcgcgatag cgtgttgatt ccagcatggt tcgtgaaaca
     6421 aagctgttat cttcctgacg gatagcacgg tgaagctcaa tagtcatttc gtttagaaca
     6481 tcattaattt ttgctaaccg ttttttaagt cgtatagtat cctgtggatt ggcattttgt
     6541 agtctttggt aaatatgttc aatttttgtc tgtacactct caggaacaaa gcggtaattg
     6601 gttataaata attccggatt attgagtaac tcaaagtttt catgcttaat taaaagatcc
     6661 tttaatgtat cagtcagcat aaaggcatga tcaaataaaa tctcaaaacc tgatgtgcca
     6721 aatatcttca gcgttaccca gggctttaag caagaaaacg ggcgcgaacc ttccacagta
     6781 aaccttccct gatcaactga atcagggcga atgatgtaat tggatgtatg catgatgttg
     6841 gtaaggtcat agggattttt aaacaaaatc agtcccatgg acaatggtga atacaacagt
     6901 ttatgagcat caaatgtaac tgaatcagcc ttttctatcc ctttgaataa atcgcggtat
     6961 ttgttaacca gaagcactga cccaccccac gccgcatcaa cgtggaagaa aatattgtat
     7021 tcatcagcaa tttttcgtaa ggtgtacagg tcatcaatat tgcctgtctc agttgtgccg
     7081 gcaatgccaa tcaaagcaat aactttaatt gtatcagcat gggtgctgtt gtactcagct
     7141 attgctttta actcacgtcg caaagcattg atatctattt tattattact atccacgggt
     7201 attttgatta cgttattatc gccaataccg gtgatacggg caaccttatc aattgaataa
     7261 tgtccacgct gcgatacaat aaacactgct ctatgtatat tataatacct gaatgcttcg
     7321 gccacacctg ctttgcgtat accgggaaag tgttcatcag caggaaatgc cttgttgcgt
     7381 gccaccagca ttgctgtaag gtttgccagt gtgccatcaa gcgttacatt tcccagtgca
     7441 attctgtgat tctgaatatt tcgtttataa aacgacgctg gcttttgaaa aataagtctg
     7501 tgtatccagg aaataaattc gcgttctaca aatgttgacg cttttgctgt ttctatcttt
     7561 acctgatttt gattgagtgc agcaatgatc atctcaagca aaatcataaa atatggaata
     7621 gcacttgtca tgtgccctat ataataggga ttgccaacct ttacagagtg ctttataacc
     7681 ttttgtttta tttcaaggag tatatctttt aaaagctttg gatcacgggg aatatcaata
     7741 ttgttaaaca ggcatgccag ctcaggcaat gatatttcgc tgtgaatacc acctttttcc
     7801 ttaaaaaatg aatgaataag gtcaagaagc tcatacccaa attgcataaa cttatcaggt
     7861 gaatcgggca tgatgaatag cttgcgtaga tgctctaaat cggtgggtat cttgccaggg
     7921 gtatgatcta ttgtgggctc agccaatctc attgtggtaa ccactcatca taaggttgga
     7981 ggacatctta ttacacaaaa gtaaatataa ttaaattaat caagtacttt tgctgaaata
     8041 tacaaaatta ttaccagtac ctatacaaat tgtgcataca ttggcgatag ttccattacc
     8101 agtccactga caatatagcc ctaaattgaa aaataaccaa ttttttggtt tttcattctt
     8161 tcaataccaa ttattgagtt tttttaaatt ctctcttgac gatgatacaa tctataatta
     8221 ccatgatgaa agttttatac gttcttttta tatttgcgtt actcaaatga caagtggtca
     8281 tccgtgattc aactggcgat ttccagttca tatcaaatgt aagaattaca gtatgacgct
     8341 cagcataatg gattgctatg tttaaaaaat tcgggattca ggacgacagt gttataaatt
     8401 ttattttgat aggagaacag ctacaatgac tattctgaaa ctggaaaatg tttcattatc
     8461 attagatgaa aagcctatac ttaataacct ttctgttgac ttctgggaag ggcatatcca
     8521 tgcaattgtt ggccctaatg gtgcaggtaa atcaacattt tcatatgttg ttatgggcat
     8581 tagtggttat agaaatatta ctggtactat ttattttaaa gggcaggcaa taaacaatct
     8641 cactgtgtat gaacgtgccc ggctgggcat aagcctggga tggcaggaac ctgcccgatt
     8701 tgaaggactt accgttgcac aatacctgaa agcatcaaaa aaagataaag gcatgtccat
     8761 tgaagaagca atggacgttg tgggactatc accacaggaa tacctgcagc gtgcatgtga
     8821 taaaaccttg agtggcggtg aacgaaaacg cattgaatta gcttctcttt tagtaatgca
     8881 acctgagctt gtgctgcttg atgaacctga ttcaggcatt gatgtggaag cgctggaacg
     8941 tatctttgat gcaataaata ttttaaagga aagaggttct acagtaataa ctatcaccca
     9001 tagtatggca gtactgaaaa aagctgagca tgcgttttta atgtgtcatg gtaacattat
     9061 tgataaggga agcattgata aaatttcagg atattttgaa aataaatgta tcccctgtga
     9121 tcataaaaat attcctgtgt taggagaagt acagtaatgg atgataagcg cgaaattgca
     9181 aaaacattat ataaaacaat ggggcttact gacgctgcct taaatgaccc ggatattgca
     9241 catctggtta taaacggcaa tatggtggtt ggggcacatt tagtgccagg gcttgttgtt
     9301 gagccacatg aaactcctaa cggtgttact gttaccatga aggtgttagc aggtagcatt
     9361 atccaaaagc cagtgcatct ttgttttggc atgcttcctg aagacggcct tcaagaaatt
     9421 atcatggatg ttaccataga agagcatgca aaagtttcta tacttgcaca ctgcacattc
     9481 cccaatgcaa aaaatattac acacaaaatg caggcaaata ttgctattaa agagggagcg
     9541 tattactcat atgtagagcg ccatgtccat gcaccaggag gtttaattac tgtagttccg
     9601 catgcgatag ttactgttga aaagaatgcc acgtttaaaa ctgattttga gcttttacgg
     9661 ggaaaagctg gtattattaa tattaattat gagacaacct gctatgaagg aagcctttta
     9721 gagatgaatg ccaaagtaaa tggtattgat gatgatgata ttaccatacg ggaaacagga
     9781 catctgaaag gccagggtgc tgttggtgta ctaaccacac gcattgccgt tcgagataat
     9841 gcaaaagcta ctgtgtacaa tacattaacc gcacatgcag catttgcacg gggacatgtt
     9901 gattgcaaag aaatcattca ggataatggt gttgcaaaag ccattcctat tgttgaggtg
     9961 tatcatccca aagcgcatgt tacccatgaa gcagccattg gcagtgttga cgataagcaa
    10021 ttgcaaacgc ttatggcacg aggtcttgat gaagatgaag cggtggattt gattattcag
    10081 ggattgttgt cgtaatagtt attttttatt tacatgtttt tctatagcaa gtgctgtaac
    10141 acccaataat gaaagcaata taaaatatat catggttttt atagaaagat cacttccctg
    10201 ctgacgacgt acaagcaaca gttcaagata catggcatca ttagaagtaa attctttttg
    10261 ctgggcaacg cggttatcaa attctttcaa aatatactca atgcctgctc ctgcttcatc
    10321 ataacggtca tatcctgcaa aaatgtgtgg gttgcgcagc ataatgtaag cctgttttaa
    10381 tttctggcta tcggtattct gtgctaaaag cttttctatt gctggttcag ttaccatttt
    10441 tgaatagata cctggtaatc caaacaaaat tcctgcaacc agtactgcta ttgaaaaata
    10501 aaaatatatt ttgctatcat caaatttcat tgtccaacct cttgtaaata tgttacgata
    10561 tcaacggttt catagtgtgc tttttccact tttaccatac cctcgggcaa caccaaagct
    10621 gcttcctgct gttttattac ttcattaata tctatctcca attcattgaa tagtggttta
    10681 attgcttctt cagattcatt ctgtgtaatg gttatctgtt gtacttgctt tggcaaaagt
    10741 ttatcagtag caaatgttaa ttgcttaaga gtatgcttat cttcagtaac tatcgccact
    10801 gttgccgtgt agttttttac aactttattt atactggtgt ttaatagggt tatggtatag
    10861 gtagtggcaa atacaggtac attttgctgg aaaaccacct gtactgaaaa ctcaacatca
    10921 cccacatcaa catactgtgt gcagccagca catacaacaa gtaatagcgg gattatgtat
    10981 tttatcatta gtacctcacc aacatatatt gtgccgttat tgtcctatta cactgattta
    11041 gtcaagtaaa atgtggaaag gtagatttaa taaaataaac gtctaccccc gcggatttat
    11101 gtgcgggggt aattcattta ttagtgtgtt ttggtattta tcgctgaatt ttttctacaa
    11161 taatttcaac atttttaaaa aaccgtgtta tatctttctt cttctctatc ccaatatacg
    11221 ccactcctaa ctcaatgtcc tgataaaacc gtgggggatt ttctataata cttccatcaa
    11281 tggcatatac tggcaaatct tcttcattat aatcatactc tacaacaagt gtataggcta
    11341 aaatcttccc ataggcagga atccttccat gccatttttg ccctacaaaa ttatgatgct
    11401 cttttattgc atcttttata gctttttgtt ttgcttctat cgatagctca ttgaaagtgc
    11461 tgggcaggtc aacctttttc aacaaaaatg attgtgtcat tgcacaaata aaaattggcg
    11521 aaggctttga atattctgtt acacagtata aaaatggttt aaagtttttg cgctcttctt
    11581 cttgtttttg ccttttttcc tcttcctgct tttgtatttc acgtacaatc tcttcttttg
    11641 tttcttctaa cttttcaagt atcttttcct ttggttcatc aagtgcacta tgtagattat
    11701 atacgatata ttcattgagt gttgccttct ctaaaaattc atcaatgcgt cttatacctt
    11761 ttgatatatt gttatatcct aaacgctgcg ctaattcttt tttggatatt ttcttttgtt
    11821 tcattgtttc ctgtaaaaat ctatgtattg cgtacattgt tttctccttt tgatataaaa
    11881 tttccttata tagaaaagcc tgttttggag tttttatttt tcatcatata ctgtacttct
    11941 ttttcaagtg catctatgat ggcatcgtgg ttgtttaa
//
LOCUS       DAORYL010000092        11751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_4190_length_11751_cov_15.472384, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000092 DAORYL010000000
VERSION     DAORYL010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11751
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4190_length_11751_cov_15.472384"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(227..1231)
                     /locus_tag="PLV81_13215"
     CDS             complement(227..1231)
                     /locus_tag="PLV81_13215"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HOM11097.1"
                     /translation="MKHIIIKNNEKYLKQEYSQEEELTNLFSKNFQNIISDKSLFIKI
                     EKQVKSKNFKNFKHSISDGFLLVWENPTTPSLYITEIELEKHDINKHILPQLGNFISF
                     IQSASIDELNVVRNFLYEEIKNNKKIFNKIQKDTGKEVHKLLDDSMEDLQILLVIDRI
                     TPELSIGLLQIEKAINVKIRKIEVSLFIKNREEVILFSDSEITEDEMQTSKERTEFEY
                     TLDYHFENKPEKILSIFNLFLEHVKTKNIKISPMKHYIGFFKNNNMIFSCVVRKNSLV
                     YYSRANINEIKSGNFNIPFRDVSSIGHYTNHLPTEIVITESDQIDDLIKYFNEIYKKY
                     "
     gene            1546..2004
                     /locus_tag="PLV81_13220"
     CDS             1546..2004
                     /locus_tag="PLV81_13220"
                     /inference="COORDINATES: protein motif:HMM:NF037895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11098.1"
                     /translation="MWTIKKYVDLEDQNNILYEFPEENKPDIDTKEESIKQENQNHKY
                     IYDVTLLDLIQSNLLENEEEIEMQYAPMVGNKKASKEDKKIYKGIINNDGKIIVNNKI
                     FDSPNHAALYCINDAGSPRKTVNGWTSWKTKNGKTLHQLRMEYLQMVNKN"
     gene            complement(2337..2549)
                     /locus_tag="PLV81_13225"
     CDS             complement(2337..2549)
                     /locus_tag="PLV81_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11099.1"
                     /translation="MRYRKWLYVFYKRMICIDVSRIFGISKHTVHRLDKEGIEEELSQ
                     QEKIRPWRINIDEISRKKGHRYATII"
     gene            2601..3179
                     /locus_tag="PLV81_13230"
     CDS             2601..3179
                     /locus_tag="PLV81_13230"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOM11100.1"
                     /translation="MALMGLRIAVVYFLIVFIGAVVSGLILEKSGAAKLVKRVRIKGG
                     INTGNNNIPAKFIMKLKASFMLAWQDFKGVLIYLFIGVGIGAAIYGYMPQTLVLRYAG
                     HDNIFAIPIAAIIGIPLYIRAETAIPIGIALMQKGMTVSAVFALIIGGSGMAIPEMSM
                     LAAIFKKRLVTAIVMVIFFTAVIGGYIFNLIF"
     gene            3220..3372
                     /locus_tag="PLV81_13235"
     CDS             3220..3372
                     /locus_tag="PLV81_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11101.1"
                     /translation="MIKENGKKPLLKKLITISKNPKSSCCSIELEEIPEANNEEKEAE
                     KKVTKN"
     gene            complement(3389..4216)
                     /locus_tag="PLV81_13240"
     CDS             complement(3389..4216)
                     /locus_tag="PLV81_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOM11102.1"
                     /translation="MKIKRYTVLFILFIGIAALIVSCSKEAREKVVVKGSTTVLPITQ
                     KAAEAFYEKNKIVISLSGTGSGDGIKSLIDGSCDIANSSREMHTEELALAKRKGEIIK
                     EVAIAFDMIVPIVHPSNPVSNLTLHQLKAIYEGSVKNWKDVGGKDETIVVISRDSSSG
                     TFEVWESKVMKHVEVRKDALLQASNGAILQTVSQNPKAIGYVGYGYLNDRVKPLYVNN
                     VEPIIENGKSGIYPIARKLYMYVNEVKIKPAAQAFIDFMLSDEGQAIVKEAGFIPVK"
     gene            complement(4339..5166)
                     /locus_tag="PLV81_13245"
     CDS             complement(4339..5166)
                     /locus_tag="PLV81_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOM11103.1"
                     /translation="MKVKSFLIFMIVAIVSSLSIGFSQTKGSKVVIKGSTTVLPIAQK
                     ALEAFYEKTNVVISLSGTGSGDGIKSLIDGSCDIANSSREMKKEEWDMAKSKGEKIKE
                     VAIAYDMIVPIVHPSNPVKNLSMAQLKAIYEGAITNWSQVGGNNERIVVISRDSSSGT
                     FEVWESKVMKKSEVRKDALLQASNGAIVSVVSKNKRSIGYVGYGYLDDRVKALTVNGV
                     EPTIPNGKSGAYPIARKLYMYVNEVKIKPEAQAFIDFILSKEGQQIVKAAGFIPLTD"
     gene            5478..6242
                     /gene="pstB"
                     /locus_tag="PLV81_13250"
     CDS             5478..6242
                     /gene="pstB"
                     /locus_tag="PLV81_13250"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOM11104.1"
                     /translation="MSESTVKVKAQNLSFFYGDNQALFDINLSVQANTVMALIGPSGC
                     GKSTFLRCINRMNDIIPYTRIEGELTIDGESIYHTKYDVTQLRRRVGMVFQKSNPFPK
                     SIFDNIAYGLKINGIKDRYTIERIVEDSLKRTALWDEVKDRLHTSAMSLSGGQQQRLC
                     IARTIAVQPEIILMDEPASALDPISTQKIEELIQDLKKQYTIIIVTHNMQQAARVSDY
                     TAFFYMGKLIEVNKTEIIFTKPEKQMTENYITGKFG"
     gene            6262..6915
                     /gene="phoU"
                     /locus_tag="PLV81_13255"
     CDS             6262..6915
                     /gene="phoU"
                     /locus_tag="PLV81_13255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOM11105.1"
                     /translation="MATHLEQELNDLLRKILEMADIAIEAVTDAVQSLKTIDVKLAEK
                     VIQKDSALDKLEIVIDEECGRILVTKQPAAGDLRLILAILKSNTDLERIGDLATNIAK
                     ETIRLEGKSLLKPLIDIPRMTEICIKMIKLAFQAITEKNIEYARQCIAMDKDVDELNM
                     QINRELFSFMVENPATISPAFGLIMVAKALERIGDHATNIAERAIYYIEGEDIRHQE"
     gene            6919..7599
                     /locus_tag="PLV81_13260"
     CDS             6919..7599
                     /locus_tag="PLV81_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOM11106.1"
                     /translation="MPKIYVVDDEKDIREILKVNLQKNGYDVYTFASAEEVLKQLAIA
                     KPDLLILDIMMSGMDGLDLCKHIRASNTLNDIPILFLSAKSAELDKVLGLELGADDYI
                     TKPFSIHELIARVKAILRRVQGKEETPKDVLTYKGIVLHPEKFSVTIDKNPIDLTKTE
                     FLILKLFMQYPGKIFTRDNIIDSIRGDNVYVIDRTVDVHIMNLRKKLGHYKDAIKTYS
                     GVGYGLKG"
     gene            7604..9337
                     /locus_tag="PLV81_13265"
     CDS             7604..9337
                     /locus_tag="PLV81_13265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM11107.1"
                     /translation="MFKNLKTKLIVVYSVALICFISFLLVYSSRHIHKEFTLFLKTEL
                     QQSIDYINTYCLSFHKPINKQFFTYEIVTALKNLSHKSSLRITIIQNDGTVLFDTDAN
                     PEIMENHLYRPEILEARKNGTGMATRFSNTIGADMLYVASYYSNYYIRTAKSMSTINM
                     LIESITRNIVIAGIIVLIISITVTAIFATAFTRPIKESLRFIDAFFNGNLHARILNYK
                     DDEMGYLQKAMNQLADNIQQRIMELTNEKNKLFMIIESIHDPIALIDEDKKIVIYNRA
                     FANCFEYPERLTGKYYFTVIRHSELNAKIYVAFSQKQKISFEETIGAKNFHIFIYPFA
                     EPATGILLLMHDVTEQKRIQQLKSDLVGNLSHELKTPISIIRGYLETIKDNICDQKTA
                     EQFIENALINVERQNAIINDMLKLNQLETSSFITSESIDLKKIISQCVELLTPKIQKK
                     NLAIVMDIQSLPQTITGNAFLAEEIFFNLIDNAINYNSPDGSITISANKESTSLSISI
                     ADTGIGIPPDEIPRIFERFYRVDKSRSRETGGTGLGLSIVKHAALILGWTVDVTSSSS
                     GSTFRIIIPSV"
     gene            complement(9341..9625)
                     /locus_tag="PLV81_13270"
     CDS             complement(9341..9625)
                     /locus_tag="PLV81_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11108.1"
                     /translation="MTKKLKKYERDILKSFEEGQWQEVSEIKKEKKNHLSYAKAAIKK
                     DKRINIRISQNDLESLQKIAMEEGIPYQTMISSVLHKYVNGKLVEVKKRP"
     gene            complement(9622..9903)
                     /locus_tag="PLV81_13275"
     CDS             complement(9622..9903)
                     /locus_tag="PLV81_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HOM11109.1"
                     /translation="MKAFKWNEEKNKLLKNERGICFEDIISCILNDKLLSIVEHKNPD
                     KYPGQKMYVIEFNRYVYLVPFVENEKEIFLKTIIPSRKATKLYLGGGKK"
     gene            complement(10014..10880)
                     /gene="pstA"
                     /locus_tag="PLV81_13280"
     CDS             complement(10014..10880)
                     /gene="pstA"
                     /locus_tag="PLV81_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOM11110.1"
                     /translation="MASLQAKAKAIEKTSFIIISALSYTILVFLLFVLGYIFVQGYRA
                     LSWEFLIAFPKNEMREGGIFPALLGTLYLMIGSSVISVPIGVMTAIYLTEYVENSKVL
                     QFIRLGINNLAGVPSVVFGLFGLSVFVIFLDFGSSIIAGSLTLGILNLPLVIRSTEEA
                     LLTVPTSYREASLSLGATKWQTIYKIVLPTALPGILTGIMLSLGRAAGETAPIMFTCA
                     TFYTPHIPDSLFSEVMALPYHIYVLAIAGTHIEATRHIQYGTAIVLIALVLGLNFTGM
                     VLRYKYRKKFKQ"
     gene            complement(10891..>11751)
                     /gene="pstC"
                     /locus_tag="PLV81_13285"
     CDS             complement(10891..>11751)
                     /gene="pstC"
                     /locus_tag="PLV81_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOM11111.1"
                     /translation="DRMVKYIFAVVGFLSVSFLFLIMIFLFKEGLPIFKVVSIKDFLF
                     GTSWYPTSEPPKFGTLPLIVASLSVTFIGAIVAVPFSLGIAIYLSELAPSFVREIVKP
                     AIELIASIPSVIIGFFGMVVVAPFLQDHFDIDTGLNLFNASIMLAFMAIPTIASISED
                     ALSSVPVSLKEASYALGANRWQTIVHITMPAALSGIWTAIILGIARVLGETMVVLMVA
                     GGATTFPTSLFDPVRPLTANIAAEMAEASYRSDHYFALFAIGIILFILTMLFNVIANY
                     LSMKYKFKYH"
BASE COUNT         3902 a         2211 c         1945 g         3693 t
ORIGIN      
        1 tggagaattg gatgagctgt ttttggaaat tattccccag cctcaaatat ttaatttttc
       61 atgttaatat atcttaggat cccagcataa aaattatttc tgaagataat gtagttgaaa
      121 ttctgcatga ttagaaccat tcacctgcta ctaagcgctt agacccgata agctggagtg
      181 caattgagtc tactatcgga aagaccaaga aataaaaaaa ttgtatctaa tattttttat
      241 agatttcatt aaaatattta attaaatcat caatttgatc tgattcagta attactattt
      301 ctgttggcaa gtgattcgta taatgcccaa tcgaactaac atctctaaat ggaatattga
      361 aatttcctga ttttatttca ttaatatttg ctcttgaata ataaaccaag ctgttttttc
      421 taactacaca tgaaaatatc atattattat tcttgaagaa acctatataa tgtttcattg
      481 gagatatttt tatattcttt gtttttacat gttctaaaaa taaattgaat attgaaagta
      541 ttttttcagg tttattttca aagtgataat ctaaagtata ttcaaattca gttctttctt
      601 ttgaagtctg catttcatct tctgttatct cactatcgct gaataatatt acttcttctc
      661 tatttttaat gaataaagaa acttcaattt ttctaatctt tacattaatc gctttttcta
      721 tttgcaataa ccctatactc aattcaggag taatcctatc aattactaac aaaatctgta
      781 aatcttccat cgaatcatct aataacttat gaacttcttt cccagtatct ttttgtattt
      841 tattaaaaat ttttttattg tttttgattt cctcatatag gaagtttcta actacgttta
      901 actcatcaat tgatgcactt tgtataaatg agatgaaatt acctaattgg ggcaatatat
      961 gtttattaat atcatgtttt tctaattcta tttcagttat ataaagcgaa ggtgttgttg
     1021 gattttccca aacaagaaga aaaccatcgc taattgaatg tttaaaattc ttaaaatttt
     1081 tacttttaac ctgtttttca attttaataa ataatgattt atcagatata atgttttgaa
     1141 aattttttga aaataaattt gttagttctt cttcttgaga atattcctgt tttaaatatt
     1201 tttcgttgtt ttttattatt atgtgcttca taatctggta atccttcaca gattgtgtaa
     1261 aagatgcttt tataaaagta ttatactgta accatataat ataattccaa aactaaacta
     1321 tattatttta acagaggata acaacgatac agacaaagag aacatactat gaagggtatt
     1381 gcaagcacaa atttttactt aattgatttg aacatgcagc ttctagtttg aagtgcaatt
     1441 aatgtagttt aaaaaaaatg actttacctt aatggattta tgagaaccta actaaaagaa
     1501 gaagacctta agctatgggc taaaacatta aaattaaatg taagattgtg gacaataaag
     1561 aaatatgttg atttagaaga ccaaaataat attttatatg aattccctga agaaaataaa
     1621 ccagatattg atacaaaaga agaaagtatt aaacaagaaa atcaaaatca taaatatata
     1681 tacgatgtaa cattgctaga cctaatacaa agtaacttac ttgaaaatga agaagaaata
     1741 gaaatgcaat atgctcccat ggtaggaaat aaaaaagcaa gtaaagaaga taaaaaaata
     1801 tacaaaggta ttataaataa cgatggaaaa ataattgtaa acaacaaaat tttcgatagt
     1861 ccaaaccatg cagcactata ttgtattaat gatgcaggaa gccctagaaa aactgttaat
     1921 ggctggacat catggaaaac aaaaaatggt aaaactttac atcaactgag aatggaatat
     1981 ctccagatgg taaataaaaa ttaacactct ttccggatta ttaagaaacc ccaaccatcg
     2041 aaaattgatg taattttttt aacattgaac tgttgaatta ccagcaacct gaaatattca
     2101 acatctttat atctgtagtc tattcgctgt ataaccctga ttttagtatt tatgccttcg
     2161 gcaaagctat ttcgataatt accacgcaat gaatttctga tattaggcaa tccagggatt
     2221 catactcact actgtgataa aatctctcca acttttatct tttcttttaa gaagagcgca
     2281 ctattcacta ctttttgccc tttaatgaca tctaaaacct tctttttatc aggtgatcag
     2341 ataattgttg cataccgatg tcctttcttt cgagatatct catctatatt gatccgccat
     2401 ggtcgtatct tttcttgttg cgacaattcc tcttctattc cttctttgtc taaacgatgc
     2461 actgtgtgtt ttgaaatccc aaatattctg gaaacatcaa tgcaaatcat ccgtttgtaa
     2521 aatacatata accatttcct atagcgcacg gtgaactgtt tatactgatt cagccattca
     2581 aaacgctctg tgtcatcata attgctttga tgggattacg gattgcagtg gtctattttt
     2641 taattgtgtt tataggagca gttgtttccg ggcttattct tgaaaaatct ggagcagcta
     2701 agcttgtgaa aagagttcga attaaagggg gaattaatac cggtaataat aacatccctg
     2761 caaaattcat catgaaactt aaagcttcat ttatgttagc ctggcaggat ttcaaagggg
     2821 tattgattta cctctttatt ggtgttggca ttggagcagc catttatggt tatatgccgc
     2881 agactttagt tttacgttat gcaggacatg ataacatttt tgccattcca attgcggcaa
     2941 ttataggcat acctctatat atcagggctg aaactgcaat ccccataggt atagccctta
     3001 tgcagaaagg gatgaccgtt agtgcggttt ttgcacttat tataggaggt tccggaatgg
     3061 ctatcccaga aatgagcatg cttgcagcaa tatttaaaaa aagactggta acagcaattg
     3121 taatggtaat tttttttact gctgttattg gcggttatat ctttaattta atattctgaa
     3181 aaaaaattat aacaattaat aaatttagga gggtatatca tgataaagga aaatggaaaa
     3241 aaaccattat taaaaaaact aatcactatt agcaaaaacc caaaaagttc ctgctgttca
     3301 attgaacttg aagaaatacc tgaagcaaac aatgaagaaa aagaagcaga gaaaaaagtt
     3361 acaaaaaatt agtgttgcta ctggttttct acttaaccgg tataaaccct gcttctttaa
     3421 ctattgcctg tccttcatct gacagcataa agtctataaa tgcttgagca gcaggtttta
     3481 tctttacttc attgacatac atatagagtt tacgtgcaat gggatatatg ccacttttac
     3541 cattttctat tattggttcc acattattca catacagcgg cttaaccctg tcattaagat
     3601 atccataccc cacatatcca atggcttttg gattttgcga tactgtctgt aaaattgccc
     3661 catttgaagc ctgcagcagt gcatctttgc gcacctcaac atgtttcatt actttggatt
     3721 cccacacctc aaatgttcct gagcttgaat cgcgtgatat aaccacaatg gtttcatctt
     3781 taccaccaac atctttccag tttttcaccg aaccttcgta aattgcctta agctgatgta
     3841 aggtaaggtt tgaaacagga tttgaaggat gaacaatagg cacaatcata tcaaatgcta
     3901 tggcaacttc tttgatgatt tcacctttgc gttttgcaag cgccagctct tctgtatgca
     3961 tttcacgcga agaatttgca atatcgcagc ttccatcaat cagcgacttt ataccatcac
     4021 cggatccagt acctgaaagg cttatgacaa ttttattttt ttcatagaat gcttcagcag
     4081 ccttctgagt aatgggtaac accgtcgttg aacctttaac cactactttt tccctggctt
     4141 cttttgaaca cgatacaata agtgctgcta tgccaataaa tagtataaat agtactgtgt
     4201 agcgtttaat tttcatcttc aaacctccat taaaaatata atttaatgtt acctcatcat
     4261 tcccaatata ttcaacactt ttttacaaaa aaacgccaca tcgctgtagc gttttttaat
     4321 aagtgtttaa tgttcacatc aatcagttaa ggggataaaa cctgctgctt ttacaatctg
     4381 ttgtccttct tttgaaagga taaaatctat gaatgcttgc gcttcaggtt ttattttgac
     4441 ttcattgaca tacatgtaca acttacgtgc tattgggtac gcacccgatt ttccgttagg
     4501 gatagttggc tcaactccat ttacagtaag tgcttttact ctgtcatcta agtatccgta
     4561 tccaacatat ccaattgaac gcttattctt tgatactacg gatacaattg ctccattgga
     4621 agcctgcaat aacgcatcct tgcgcacttc agatttcttc ataacctttg attcccacac
     4681 ttcaaacgtt cctgaacttg aatcacggga tattacaact atcctttcat tgttgccacc
     4741 cacttgactc cagttggtta tagcaccctc atagatggct ttaagctgag ccattgacaa
     4801 attttttaca gggtttgaag gatgcacgat tggaactatc atatcgtatg ctatagcaac
     4861 ttctttaatc ttttcacctt ttgattttgc catatcccac tcttccttct tcatttcacg
     4921 tgaagaattt gcaatatcgc agcttccatc tataagcgac tttataccat caccagaacc
     4981 tgtacctgaa agacttatta caacatttgt tttttcataa aaagcttcca gtgccttctg
     5041 agcaatgggc aaaacggtag ttgatccttt gatgactacc tttgatcctt ttgtttgtga
     5101 aaaaccaatt gataatgagc ttactatcgc tactatcatg aaaattaaaa aactcttgac
     5161 cttcattatg tcctcctgtt atattttagt aaggtaatgt aaaacgcaat tatgcaattt
     5221 cacactattt taagtacact ggtattatga aatttttata aagattagat taattttcta
     5281 tttgatttga cctgaattga tcttttcatc atatacaaca ataagtccag gcggcaaaaa
     5341 gcactgctat agtactaaaa ctttacattt tctttacatt attattataa atccttaaca
     5401 ttccagtggt ttgatacttt tgataattaa aagtaatgat acatacaacg tactagttaa
     5461 agaaataacg gtgatatatg tccgaaagca cagtaaaagt taaagcccag aatctttctt
     5521 ttttttacgg ggataatcag gcattgtttg atattaatct atcagtacag gcaaacacag
     5581 ttatggcact gattggtcct tctggatgtg gtaaatcaac atttctacgc tgcatcaaca
     5641 ggatgaacga tatcataccc tatacaagaa ttgaaggcga actaaccata gatggagaat
     5701 ccatatacca tacaaaatac gatgtcacac aactgcgtcg ccgcgttggc atggttttcc
     5761 agaaatcaaa tccattccca aaatcaattt ttgacaacat agcgtatggt ctaaaaataa
     5821 atggtataaa agatagatat accattgagc gtattgtgga agactctttg aaacgcacgg
     5881 cactgtggga tgaagtaaag gacaggctgc atacgtcggc catgagcctg tcaggtggac
     5941 agcagcagcg cctgtgcatt gcacgaacaa ttgcagtgca gcctgagatt attctgatgg
     6001 atgagccagc ttcggcactt gacccaatat ccactcaaaa gattgaagag cttattcagg
     6061 acttaaaaaa acaatatacc ataattatcg ttacccacaa catgcagcag gcagcacgcg
     6121 tgagcgacta taccgcattc ttttatatgg gaaaacttat tgaagtaaat aaaaccgaaa
     6181 ttatatttac aaaacctgaa aagcaaatga ctgaaaatta tataacagga aaatttggat
     6241 aatcattgaa aggagatacc aatggccact cacctggaac aggaactcaa tgacttactg
     6301 cgcaaaatac tggaaatggc tgatattgcc attgaagcag ttactgatgc agtgcagtca
     6361 cttaaaacta tagatgtaaa gttggctgaa aaagtgatac aaaaagactc agcccttgat
     6421 aaattggaga tagttattga tgaagaatgt ggcaggattt tagtaaccaa acaaccagca
     6481 gcaggcgacc tcaggttaat tttagcaatc ctgaaatcca acactgattt agagcgcatt
     6541 ggcgatctgg caactaatat tgctaaagaa acaattcggc ttgagggcaa gtcgctttta
     6601 aagccactta ttgatattcc ccgtatgacg gaaatatgta ttaaaatgat taaactggca
     6661 tttcaggcaa tcaccgagaa aaacattgaa tatgcacgcc agtgtattgc catggacaaa
     6721 gatgttgatg aactcaatat gcagataaac cgcgagcttt tcagctttat ggtagaaaat
     6781 ccagcaacca tttcacctgc atttggcctt atcatggtag caaaagcatt ggaacgcatt
     6841 ggcgatcatg caaccaacat cgctgaacga gcaatatact atatagaagg cgaagacatt
     6901 cgtcatcagg agtaattcat gccaaaaata tatgtagttg atgatgaaaa ggacatccgc
     6961 gaaatcctta aagtcaacct ccagaaaaac ggctatgacg tttatacatt tgcttcagct
     7021 gaagaggtcc taaaacaatt agctatcgcc aaacctgatc tgttgatact tgatatcatg
     7081 atgagcggca tggatggatt ggatttatgc aagcacatca gggcttcaaa tacgttaaac
     7141 gatataccca tattattcct ttctgcaaaa tcagcagaac ttgataaagt gttgggactt
     7201 gaactggggg ctgatgatta cataaccaag cctttcagca tccatgaact cattgcccgt
     7261 gttaaagcaa tactgcggcg tgtccaggga aaggaagaaa caccaaaaga cgttttaacc
     7321 tacaaaggca ttgtactgca tccagaaaaa ttttcagtaa ctatcgacaa aaaccccatt
     7381 gaccttacaa aaactgaatt tcttatcctt aaacttttta tgcaataccc tggcaagatt
     7441 tttacccgtg acaacatcat cgacagcatt cgcggcgata atgtatatgt cattgacagg
     7501 accgttgacg tgcacattat gaatctcaga aaaaaattag gacactacaa ggatgcaatt
     7561 aaaacctatt ctggcgttgg gtacggatta aaagggtaag ttaatgttta aaaatcttaa
     7621 aacaaaatta attgttgtat actctgtagc actgatatgt tttatttctt ttcttcttgt
     7681 ttacagtagt cgccatatac acaaagaatt tactctattc ttaaaaactg aattgcagca
     7741 aagtattgat tatataaata cctattgcct gtcatttcac aaacccatta acaaacaatt
     7801 ttttacttat gagatagtaa ctgctctaaa aaatctatcc cataaaagtt ccctgcgcat
     7861 aaccattatt cagaatgatg gcactgtact gtttgacacc gacgccaacc ctgaaataat
     7921 ggaaaaccat ctgtatcgcc cagagattct ggaagcaaga aagaatggta caggtatggc
     7981 aacgcgattc agcaatacaa tcggtgctga catgctatac gttgcttcat attatagcaa
     8041 ctactatatc cgcactgcaa aatcaatgtc cactattaat atgctcattg agtcaataac
     8101 cagaaatatt gttatagctg gaattatcgt ccttattata agcattaccg tcacagcaat
     8161 atttgccact gctttcacac gacctataaa agaatcactg cgcttcattg atgcattttt
     8221 taacggaaac ttgcatgcgc gcattctcaa ctacaaagat gatgaaatgg gctacttaca
     8281 gaaagcaatg aaccagttag cggacaatat ccagcagcgc attatggaac ttaccaatga
     8341 aaaaaacaaa ctcttcatga ttattgaaag tatccatgac cccatagcgc tcattgatga
     8401 agacaaaaaa attgttattt ataacagggc atttgcaaac tgttttgagt atcctgaacg
     8461 tttaactggc aaatactatt ttactgttat ccgccacagt gagcttaatg caaagatata
     8521 tgttgcattt tctcaaaagc aaaaaatatc ttttgaagaa accattggtg ctaaaaattt
     8581 tcatatattt atatatccgt ttgcagaacc tgccacaggc atcctgttac ttatgcacga
     8641 tgttactgaa caaaagcgca tacagcaact gaaatcagat ctggttggga atttatcgca
     8701 tgaattaaaa acgccaatca gtatcatccg cggatacctg gaaaccatta aagacaacat
     8761 atgcgatcaa aaaacagcag agcaattcat tgaaaatgca cttataaacg ttgaacgcca
     8821 gaatgcaatc atcaacgata tgctaaaact caatcaatta gaaacttcat cgtttattac
     8881 cagtgaatct attgacctta aaaaaatcat ttcacagtgt gtagaactgc taacaccaaa
     8941 aatacaaaag aagaatctgg cgatagttat ggatatacaa tctctgccac aaaccattac
     9001 tggcaatgca ttcttagccg aggagatttt tttcaactta atagacaatg ccattaacta
     9061 taacagtcca gatgggtcga taactatctc tgcaaataaa gaatcaacat cgctttcaat
     9121 aagtatagca gacaccggca ttggcattcc acctgatgaa ataccaagga tttttgaacg
     9181 cttttaccgg gttgacaaaa gccgctcccg cgagaccggc ggtactggtt tgggcctatc
     9241 cattgtaaaa catgcagcgc ttattttggg atggacagtg gacgtaacat cgtcatccag
     9301 tggttctaca ttcaggatta ttattccatc agtataataa tcaggggcgc tttttaactt
     9361 ctactaattt tccattgaca tatttatgaa gcacactgga aatcattgtt tggtaaggta
     9421 tcccttcctc catggcaatt ttttgcaaag actcaagatc attttgtgaa atgcgtatat
     9481 taattcgctt atcttttttt attgcagctt ttgcatagct taaatgattc tttttttctt
     9541 ttttaatttc actaacttct tgccattggc cttcttcaaa tgatttgaga atatcccgtt
     9601 catatttttt caatttttta gtcatttctt acctcctcct aaataaagtt ttgttgcctt
     9661 tctgctggga ataatagttt taagaaaaat ttctttttca ttttcaacaa aagggactaa
     9721 ataaacatat ctattaaact caatgacgta cattttttga ccaggatatt tgtcaggatt
     9781 tttgtgttca acgattgaca ataatttatc atttagtata caacttatga tatcttcaaa
     9841 acaaatacct cgctcatttt taagaagttt attcttttcc tcattccatt taaaagcttt
     9901 catttaatag taaatatatg ccaatgtgtg ccttatgtca acatatattt acaaaataat
     9961 gattgggcat gcatagtatc ccttttttct gtttcaggtt tataatgcta ttgttattgt
    10021 ttaaatttct tcctgtattt ataccgcaaa accatgccgg tgaaattcaa gcccagtacc
    10081 agtgcaataa gcacaatggc cgtaccatat tgtatatggc gcgttgcctc aatatgggtg
    10141 ccagctattg ccagcacata gatgtgatat ggcagtgcca taacctcgct aaagagtgaa
    10201 tcgggtatat gcggggtata gaaggttgca caggtaaaca ttatcggagc tgtctcccct
    10261 gctgctctac ccagtgacag cataataccg gtcaatatgc ccggcaatgc agtaggcagg
    10321 actatcttat aaatagtttg ccatttggtt gcccctaacg aaagcgatgc ctcgcggtat
    10381 gatgtgggaa cagtgagcag tgcttcttca gttgagcgta tgaccagtgg caaatttaaa
    10441 atccccagtg tcagtgagcc agcaatgata gatgaaccaa aatctaagaa tattacaaat
    10501 acactcaatc caaaaagccc aaacacaact gacggaacac cagccagatt attgatacca
    10561 agacgaataa attgcagcac tttactattt tctacatact ccgttaaata gattgcagtc
    10621 ataacgccaa taggtacaga tataaccgat gaaccaatca taagatacaa tgtgcccaga
    10681 agtgccggga atatcccccc ctcacgcatt tcgtttttgg gaaatgctat taaaaattcc
    10741 cagctcaatg cacggtaacc ctgcacaaat atatatccca acacaaacaa taaaaatact
    10801 agtatggtat atgacagggc actgatgatt ataaaagagg ttttttcaat tgcttttgct
    10861 tttgcctgta atgatgccat agttcccttt ttaatgatac ttgaatttat atttcattga
    10921 aagatagtta gcaataacat taaaaagcat ggtcagtata aataaaataa tgcctattgc
    10981 aaagagggca aaataatggt cgctgcgata tgatgcttca gccatctcgg ctgctatatt
    11041 ggcagttaac ggccgcacag ggtcaaagag cgaggtggga aacgtagttg caccaccagc
    11101 aaccatcagc accaccatgg tttcgccaag gacacgtgca atccccaaaa tgatagccgt
    11161 ccatataccg gaaagcgctg ccggcatggt tatgtggaca atcgtttgcc agcggtttgc
    11221 tcccagcgcg tacgatgctt cttttagcga taccggtacc gatgacaatg catcttcgga
    11281 tatgctggca atagttggta ttgccataaa agccagcatg attgatgcat taaaaagatt
    11341 gagaccagta tctatatcaa agtggtcctg taaaaatggt gcaacaacca ccataccaaa
    11401 aaagccaatg ataaccgaag gaatggatgc aataagttca attgccggct taacaatttc
    11461 acgaacaaac gatggagcta attctgacag ataaattgca atacccaaac tgaatgggac
    11521 agcaacaatt gctccaataa atgtaacgga taaagacgct acaataagtg gcaatgttcc
    11581 aaattttggc ggttccgatg ttggatacca gcttgtccca aaaagaaagt cctttatact
    11641 gactaccttg aaaattggta aaccttcttt aaagagaaaa atcatgatta aaaataaaaa
    11701 tgaaactgaa agaaagccaa caacagcaaa aatatatttt accatcctgt c
//
LOCUS       DAORYL010000093        11469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_4317_length_11469_cov_14.275626, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000093 DAORYL010000000
VERSION     DAORYL010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11469
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4317_length_11469_cov_14.275626"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            345..1049
                     /locus_tag="PLV81_13290"
     CDS             345..1049
                     /locus_tag="PLV81_13290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOM11112.1"
                     /translation="MVTVGNNVFSLTHLQSLLKYFKVWHLILLYTRLKDTMLDKTPEQ
                     ICVEYIDFIIKTIKKINSIKNYKLDNDDINDIRQNVITKLLGGGLAQFKGLCKLDTYI
                     YKIVYNEIQTYLRRYEKETPVPSTVLQSNYDQDEDDAYNNFFDYFHNFNIDYTDKIFR
                     QDIIAIIDEELSTFDYIDKLIFTWFFLCKRSQIQIAERVKLSQPTVSEHIEKIKKRLR
                     TAIKRKYPEFEKELKS"
     gene            1046..1606
                     /locus_tag="PLV81_13295"
     CDS             1046..1606
                     /locus_tag="PLV81_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11113.1"
                     /translation="MIRAHLQVDCNDEMQEKVLGVADGTISDEKTIQEVLEHLSMCKE
                     CFALYDNYFLLMEHPELDEQIEDDILSLDFNIANNTFVPVTSSTHIIPPLVAVLAQPD
                     VPAVEIEYPFKGSIIKIRIIYGTNHNIDISIYCNNDGIKIYLLNGSRFDIATVSNGMA
                     MFSSVEMGVILLLIDFKKVIRIHINN"
     gene            1611..3653
                     /locus_tag="PLV81_13300"
     CDS             1611..3653
                     /locus_tag="PLV81_13300"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HOM11114.1"
                     /translation="MSYLYLDESGQFRGQKSRRSIVGGIFSKHKNITDSAVRAFFKQF
                     GYDNTPFHGKDLDNATLSKIIDALIDFCKTNEFEPVIFIPRRDFFVIDDTVTYINVLT
                     DSVTKFLIKKAQLINDITIVIEHRVNLLEDSYKERINEAIAKTVALYGSINKHITCDV
                     YIQNKDNVFLQIADAIVHTFYRLDVSYDSDDTEFDENVKQKFKQWIEPYKIYIHGASS
                     PSEKIKDMIADGDYIQAVMYIANHQKRDKDVQKIIPSIIERLCQLNVLHLNTIMYSVL
                     ALCYDNVNVKRLLDDFEIYVIFLLKNFLPELQNKLSQYHKQPEDIQWAFCYCYMILLT
                     LYNHKGDPRSFEQIYTQAQQYIASSTFDFDFLSARLRIPILYGVHLTNQFNFEKAYDV
                     MITLQRKIEDAFAFINESDENLTVKPRIIGEIIGTGLQALMYKTLQHNGNWDEVRELS
                     NKAIDSFEYSEDIKRQYQYRAQIETYAGDSQQARIYLAKSVGIEYTSDAELLETILKL
                     EREGAFPLLHFLRMYYVDVMKEPDTHVKHYYEIVTAAIAKYASSFQQIIRINAYPLHS
                     ILHYLMIVYMLNNSNASRKEAVEYYQKAQDLCKRNDAITIKTIEISLEADYLWLQIYI
                     DSKDVIKNKDNIMKKFDKLIKLSEGMTVHSYIAKAKEKYKITPVEKWNTLWNMFPY"
     gene            complement(3683..5278)
                     /locus_tag="PLV81_13305"
     CDS             complement(3683..5278)
                     /locus_tag="PLV81_13305"
                     /inference="COORDINATES: protein motif:HMM:NF021207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11115.1"
                     /translation="MQNLKTLADELIAIKDKKQRFSFYRDTILPVVAKGLHEQYLHYL
                     KINNLNPYHTLVTTLSHNLNITVLLVQTIKPQCCVVFYTPDKQKNMDLFSEFCKTGTI
                     ECLPVKLDEINNTANSETIKNVLTTYSKQHKSILCDITGGKKILSFQIGIIAHSLKLD
                     ITYIDSQKGFIEWGIPYPGHEVLYIQKPDNVITLLQINPINKLRIRFDKNNNTIIYEA
                     DYDNRFYRLGTKSFKNSESETNTLISYINKFFIEIDASILHNTFTFDMLKNLSLSIQS
                     LLFSKELKKFLKKNKTINQLVVDEELCAIPWEILLLHYNIPLPLVRIPNRVLNENIDV
                     SLHNVEKRIALISGSGHGIPGFDSFMQNIKHFCLKQTSLNINTFEAKSSFAMKSFFAQ
                     NTSTPLTLVIYYGHAQCGTTPETTGLVCQDGSIFSINDCDILADAPPQCMLVNACQSA
                     RGNLFSDISFAHAVLKEGVGTFIGTHFFLQYEQSKEFLQCFLKSIMQNKTYLQSFKDS
                     LVALQQTFGNHDISTYNYVYYGD"
     gene            complement(5356..5781)
                     /locus_tag="PLV81_13310"
     CDS             complement(5356..5781)
                     /locus_tag="PLV81_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11116.1"
                     /translation="MVEYILYAVVFVLSFRFLYELSPNPIKNKILFYFSDIVSLFTEY
                     LGKLIIGQPIYSMCIEFVDEHTTDNEKANDNPQENTEKIETIAQTLVGLLAFNSYDIN
                     EFKSIIQEMQNITQETTQKKYNKYKASMMVVYTRHPDLL"
     gene            complement(5925..9359)
                     /locus_tag="PLV81_13315"
     CDS             complement(5925..9359)
                     /locus_tag="PLV81_13315"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HOM11117.1"
                     /translation="MNTIQINNECAIDLYSKLEEIVSIEIKNKDTIDEEKTKIYKVKE
                     FRLILEKLFSELAKNMPDSELINHAARMEYVFKTSTADNDIRKKCYSLRAQLNPFHHY
                     GYPDSVSNEKYLILLKTLCQIINYFSKCDIPRKLKDIYQSVAHLELPDKPEIPKEKIE
                     FVQASIYKIKKENNQLYITCIDQHNLNYMHIYINKNNSLFIHLKEVLWENATINIFNI
                     FKTPDKPFYSVNPQSFFIIEPDYLINITQIKECFVTWNKVIPSLYLLNKFELFKPSIE
                     KIQGNIANFILDTLVFTGNTKFDDLFNKATREYAFDIVSSVKAEDLPETIRKLKENSE
                     KHFNSIITFVNSEKVKNIKTSKTKSNNLIHIEPYVISHMYGLSGRFDILLEYQRDDTT
                     VCRDIIELKSTKNPPKNDAPYINDNIQVFCYDIMLNSMYPDRVGESLVLYSSKQDNCL
                     ISIKKNFENNPNINITQDIVKARNCIVHYEYLLAKGELLIDSIVEVIKNDVSEYKSFK
                     KDFADGFINTINNLENNLEKKYINNFLQFIAKEQWINKLGKSDDIEYNKYYGFSSLWK
                     LDINEKQDHNIIFADLEYDEANSNLEQLTVCFKRNLFNNPYLSTVFREGDVCIIYPQD
                     SNLLHSDDPTKKIFLKATIATIESDTIIVKLRNLYPSKNYFKKHSTWCIEQDLTDSFS
                     EKLSKSLYEFAKCDKDKKELLLGLKEPQFEDPNTYNVIYTDDHIIKTAIEKSVSCKDY
                     FLIQGPPGTAKTSIVLINIVKHIYNTTQENIFIMAYTNRAVDEICENLIKHSINFIRL
                     GRGFKYPDYNLDHIKYTMDIYQLYNKIHNCRIFVSTVHSAYESPIFNMKSFHTAIIDE
                     ASQLLEPHIIGILTRFNRFIMIGDEKQLPPVVVQEQKNVDDDELKKISLYSLSNSLFE
                     RLLLNAKRKKWDNASIMLQKHYRMHEEIADFVNTHYYNNLLKIGSNSQKQAINIFKSN
                     FENKIERLLSKHRIIFIQSDKDKGKTNKYEANIVKDIVKVIINVSNDFTEKTVGIITP
                     FRAQINLIRKTLFDSQIPKEIIDKITIDTVERFQGSERDIIIVSFSLNSYKNIDLISS
                     LTFNRKVDRKLNVTLTRAREHLILTGCAQVLEHSQHYKDLLNYIKQKGGFVESKIL"
     gene            9686..10699
                     /gene="cas1"
                     /locus_tag="PLV81_13320"
     CDS             9686..10699
                     /gene="cas1"
                     /locus_tag="PLV81_13320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HOM11118.1"
                     /translation="MLTVYVYEQGAKITKEGEVLTVNMPDGTTQNVRINNIDRIIIYG
                     NVQFTTQALTLLLWDNIPVSFLTLNGEYKGSLVTADGANCFRRIKQYEILNNKEKRLA
                     LARRIVYAKLYNGYVFIRNYHHHKTLENFGKYNHIIENVIQRCKIAATIQELMGYEGF
                     GTKSYFAMYTALFDKQWNFTKRTKRPPMDPVNAMMSLGYTLVSTEIASLLESHGLDTS
                     LGMYHSLEYGRQSLALDIVEEFRAVLVDRFVFQLLSQKIFKPDDFEYDEQKGCRFKKE
                     ALKEYLVRYNKLLNTDLGLKEPVTWRQLLWRQVERMVAFIDKDVEYMPLLYGEVYESS
                     SGI"
     gene            10677..10952
                     /gene="cas2"
                     /locus_tag="PLV81_13325"
     CDS             10677..10952
                     /gene="cas2"
                     /locus_tag="PLV81_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015169679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOM11119.1"
                     /translation="MRVVVVYDVRDNRRRKRLHTLLKGYGQWQQYSVFECELSQKKYF
                     ELVSRVKYIIDESEDSVLFYKLCAMCVTQTQSIGTAILYEEEEVLVI"
BASE COUNT         3981 a         1585 c         1907 g         3996 t
ORIGIN      
        1 aaatccactg tgcattaaat aaaatttttt tacgcagata tcgcagagta cggaaagaat
       61 aatcagtgtt ttatgtcatt atgattcctt gtagaaactg gtaaatttag agaaaatgat
      121 gcaagaatgt ttagtatggc ttaaagagct actaatagca atgaatgctg gtgctatcgc
      181 acataatata cacagaatga aaaaaataaa ataattcata tagacaccat gcaaccattt
      241 taactggcat tagcatgtaa aaaaatacca ataatataac gccattaatt gtctaaataa
      301 gtaaataatg gtaatacagg ttttaataac attaagggta aactatggta actgtaggga
      361 ataatgtttt ttccttgaca catcttcaat cattgcttaa gtactttaag gtatggcact
      421 taatactatt atatacaagg ttaaaagata ctatgcttga taaaactcct gaacagatat
      481 gtgttgagta tatcgatttt attattaaaa caattaagaa aataaatagt atcaaaaatt
      541 acaaattaga taatgatgat attaatgata tacgtcaaaa tgtcataaca aaacttcttg
      601 gtggcggact ggctcaattt aaaggtctgt gtaagttgga tacgtatatt tataaaattg
      661 tttataatga aatacaaaca tatttaagaa gatatgaaaa agaaacccct gtcccttcca
      721 cagtgcttca atcaaattat gaccaggatg aagacgatgc ctataataat ttttttgatt
      781 acttccataa ttttaatata gattatactg ataaaatttt cagacaggat attattgcta
      841 ttattgatga agaattgagt acatttgatt atattgataa attaattttt acctggtttt
      901 ttttatgcaa aagatctcag atacagattg cagaaagagt taaactatcc cagccaaccg
      961 tttcagagca cattgaaaag ataaaaaaac gcctccgcac agcaataaag cgcaaatatc
     1021 ctgaatttga aaaggagttg aagtcatgat aagagcacat ttgcaagtgg attgtaatga
     1081 tgaaatgcaa gaaaaagtac ttggtgttgc tgatggtact atttctgatg agaagacaat
     1141 acaggaggta ttagagcatc tatcaatgtg caaagagtgc tttgcattat atgataacta
     1201 ctttttgctt atggagcatc cagaacttga tgaacagata gaagatgaca tactctcgct
     1261 ggattttaac attgctaata atacatttgt tcccgtaaca tcttctacac atattattcc
     1321 accgctggtt gctgttttag ctcagcccga tgtacctgct gttgaaattg aatatccgtt
     1381 taaggggtct attattaaga tacgcattat ttatggtaca aatcacaata ttgatataag
     1441 tatatattgt aataacgatg ggataaaaat ttatttactt aatggtagca ggtttgatat
     1501 agcaacggtt tccaatggaa tggctatgtt cagttcagtt gagatgggtg ttattttgct
     1561 tttgattgat tttaagaaag taataagaat acatataaat aattaatttt atgagttact
     1621 tataccttga tgaaagtggc caatttaggg gacaaaaaag ccgaagaagt attgttggcg
     1681 gaatattttc taagcataaa aacattactg attctgccgt cagagctttt tttaagcagt
     1741 ttggatatga taatactcct ttccatggaa aggatcttga taatgccacc ctttcaaaga
     1801 taattgatgc attgattgac ttttgtaaaa ctaatgagtt tgaaccagtt atatttatac
     1861 ccaggcgtga tttctttgtt attgatgaca ctgttacgta tattaatgta ttaaccgata
     1921 gtgtaacaaa gtttttaatt aaaaaagccc agcttataaa tgatataact atagtaattg
     1981 agcatcgcgt taatttactt gaggattcct ataaagaaag aataaatgaa gcaatagcta
     2041 agactgttgc attgtacgga tccattaata aacatattac ctgtgatgta tacatacaga
     2101 ataaagacaa tgtatttctt cagattgctg atgcaatagt acatactttt tacagattag
     2161 atgttagtta tgattcagat gatactgaat ttgatgaaaa tgttaaacaa aagtttaaac
     2221 agtggattga accgtataaa atttatattc atggagccag ttcacccagt gaaaaaatta
     2281 aagatatgat agctgatggt gattatatac aggcagttat gtatatagcc aatcatcaga
     2341 aaagagataa agatgttcaa aaaataattc catctataat tgaacgtctt tgccagttaa
     2401 atgtattaca ccttaatact attatgtaca gtgtattagc attgtgttat gataatgtaa
     2461 atgtaaaaag gttgttagat gattttgaaa tatatgtaat cttcttatta aaaaactttc
     2521 tgcctgaact tcaaaacaaa ttgtcacaat accacaagca acctgaagat attcaatggg
     2581 cgttttgtta ctgttatatg attttgctga cactgtataa tcataaaggt gatccccgta
     2641 gttttgaaca gatttataca caggcgcagc aatatattgc atcatcaaca tttgattttg
     2701 attttttaag tgcacggttg aggataccta ttttgtatgg tgtgcatttg acaaatcagt
     2761 ttaattttga aaaagcttat gatgttatga tcacgcttca aagaaagatt gaagatgcat
     2821 ttgcatttat aaatgagtca gatgaaaacc tcactgttaa accacgcata attggtgaaa
     2881 taattggcac aggcttgcag gcattgatgt ataaaacctt acaacataac ggtaattggg
     2941 atgaagttcg tgaactttcc aataaagcaa tagacagctt tgaatattca gaagatatta
     3001 agcgccagta ccagtacagg gcacaaattg aaacgtatgc aggtgatagc cagcaggcac
     3061 gcatatattt agccaaaagt gttggaattg aatataccag tgatgctgaa ttactggaaa
     3121 caatattgaa attagaacga gagggggcat tcccattgct gcacttttta cgcatgtatt
     3181 atgttgatgt aatgaaagaa cctgatacac atgtaaaaca ttattatgaa atagttactg
     3241 cggcaatagc caaatatgca agtagtttcc agcaaattat aaggattaat gcgtatccat
     3301 tgcacagtat tttgcattat ttaatgatag tgtatatgct taacaattca aatgcttcca
     3361 gaaaagaagc tgtagagtat tatcaaaaag ctcaggattt atgcaaacgc aatgatgcaa
     3421 taacaataaa aacaattgag atttccctgg aagctgatta tttatggttg cagatatata
     3481 tagattcaaa agatgtaata aaaaataaag acaatataat gaaaaagttt gataaactta
     3541 tcaaattatc agaagggatg acggttcata gctatattgc aaaagcaaaa gaaaaatata
     3601 aaattacacc agtagaaaaa tggaatacat tgtggaatat gttcccttac taaaacaatc
     3661 agtctaaaac agttaaaaaa attcaatcac cataatatac gtaattatac gtacttatat
     3721 cgtgattgcc aaatgtttgt tgtaatgcaa caagactatc cttaaaagac tgtaaatacg
     3781 ttttattttg cattatagat tttaaaaagc actgtaaaaa ctccttgctt tgttcatatt
     3841 gtaaaaagaa atgtgtgcca ataaaagtgc ctacaccttc tttcaacaca gcgtgcgcaa
     3901 aagaaatatc tgaaaaaaga ttaccacggg ctgattggca ggcgttaacc agcatgcatt
     3961 gtggaggtgc atcagctaaa atgtcgcagt cattaattga aaaaatgctg ccatcctgac
     4021 atacaagacc agttgtttca ggggttgtgc cacattgagc atggccgtaa taaataacta
     4081 aggttaatgg tgtacttgta ttttgagcaa aaaaggattt cattgcaaat gaacttttag
     4141 cttcaaatgt gtttatattc aacgatgttt gttttaaaca aaaatgtttt atattttgca
     4201 tgaatgagtc aaaaccagga atgccatgac cagaaccact aattaaggct atcctttttt
     4261 ctacattatg taacgatacg tcaatatttt cattcaaaac acgattggga atacgtacca
     4321 atggtaatgg aatattgtaa tgtaaaagca agatttccca tggtatggca cacaactctt
     4381 catcaacaac cagctggttg atggttttgt tttttttaag gaattttttt aattctttac
     4441 taaaaagaag gctttggatg gacaatgaaa ggtttttaag catatcaaaa gtaaatgtgt
     4501 tgtgaagtat tgatgcatct atttcaataa agaatttatt aatatagcta atcaatgtat
     4561 tagtttcaga ttcactattt ttaaaggatt ttgttcctaa acggtaaaaa cgattatcgt
     4621 agtcagcttc atatataatg gtattattat ttttatcaaa acgtatcctc aatttgttaa
     4681 ttggatttat ttgtaataag gtaataacat tatcaggttt ttgaatatat aaaacttcat
     4741 gcccaggata gggaatgccc cattcaataa accctttttg agaatcaata taagtaatat
     4801 ctaactttaa tgaatgagca attatcccaa tttgaaagga tagtattttt tttccaccag
     4861 taatatcaca caaaatggat ttatgttgct tactatatgt tgtcaggaca ttttttatgg
     4921 tttcactgtt ggctgtgtta tttatttcgt ccagtttaac tggtaggcat tctatggtac
     4981 ctgttttgca aaattcagaa aagagatcca tatttttttg tttatcaggt gtataaaaaa
     5041 caacacaaca ctgcggctta atggtttgaa ccagtaatac cgttatgttc aggttatggg
     5101 aaagtgttgt aacaagggtg tgatagggat ttagattatt aatttttaaa tagtgcagat
     5161 attgttcatg taaaccttta gcaacaacag gtaaaatagt atcacggtaa aaagaaaacc
     5221 tttgcttttt gtctttaatt gcaattagtt catcggcaag tgttttcagg ttttgcatgc
     5281 aaaaagtata agaaatgcct gttggacaat gtcaagaata aattcataat gtcaatatca
     5341 ctttagtata cacttctata acaaatctgg atgccttgta tagacaacca tcattgaagc
     5401 cttatattta ttatattttt tttgtgtggt ctcttgagta atgttctgca tttcctgaat
     5461 aatgctttta aattcattaa tatcgtatga attgaaggct aacaaaccta caagcgtttg
     5521 agctattgtt tctatttttt cagtattttc ttgtggatta tcattagctt tttcattatc
     5581 agtagtatgt tcgtcaacaa attcaataca catgctgtaa attggctgcc ctattattag
     5641 tttacccaga tattcagtaa agagggaaac aatgtcagaa aagtaaaata aaatcttgtt
     5701 ctttataggg ttgggcgata gttcatataa aaacctgaac gataacacaa aaaccactgc
     5761 atataatata tattcaacca tacgcacctc atgtgtataa aatttaatga tgttttaata
     5821 gagtatatat aattagatgg gaagtatgat gaaaatatag gaaaaataat gtgaaaagta
     5881 gatcatgtat taatgatccc tacaaaaaaa tgctataatt tttattataa tatttttgat
     5941 tcaacaaatc cccctttttg ctttatgtaa ttaaggagat ctttataatg ctgactatgt
     6001 tccagcacct gagcacatcc tgttaatatc aagtgttctc ttgcacgtgt caatgtaaca
     6061 tttaattttc tgtcaacttt cctattaaaa gtaagtgaac tgataagatc aatattttta
     6121 taactattta aagaaaacga tactataata atatctctct cgcttccctg aaagcgttct
     6181 acagtatcta tggttatttt atcgataatt tcttttggta tctgactgtc aaaaagtgtt
     6241 ttgcgtatta aatttatttg tgccctgaat ggtgttataa tacctactgt tttttcagta
     6301 aaatcatttg atacgtttat aataacttta acaatatcct ttactatatt ggcttcatat
     6361 ttatttgttt tacctttgtc tttatcagat tggatgaaaa ttattctgtg tttagaaagc
     6421 aacctttcaa ttttgttttc aaaattactc ttgaatatgt taattgcttg tttttgtgag
     6481 ttactcccaa tctttagaag attattatag taatgtgtgt ttacaaagtc agctatttct
     6541 tcatgcatac gataatgttt ttgtaacatt atagaagcat tatcccactt tttacgttta
     6601 gcatttagta ataacctttc gaataatgaa ttggaaagag agtataacga tatctttttt
     6661 aactcatcat catcaacatt tttttgctcc tgaaccacta caggaggtaa ctgcttttca
     6721 tcgccaatca ttataaaacg gttaaaacgg gttaaaatgc caatgatgtg aggttcaagt
     6781 aactgtgaag cttcgtcaat gattgcagta tgaaaagatt tcatattgaa tatgggactt
     6841 tcataagctg aatgaacggt gctaacgaat attctgcaat tatgaatttt gttgtacaat
     6901 tgatatatgt ccatagtata tttaatgtga tctaagttat aatcaggata tttgaaacca
     6961 cgtcctagtc tgataaaatt aattgaatgt ttaataagat tttcacaaat ttcatctaca
     7021 gccctgtttg tatatgccat gataaaaata ttttcttgag tagtgttata gatgtgtttt
     7081 acgatattga taagaactat agatgttttg gctgttccag gtgggccttg aataagaaaa
     7141 tagtctttgc atgatacaga tttttctata gctgtcttga ttatgtgatc atcggtataa
     7201 ataacgttat atgtattggg atcttcaaat tgaggttctt ttaaacccaa tagtaattct
     7261 tttttatctt tatcacattt tgcaaattca tataaagatt tagaaagctt ttctgaaaaa
     7321 gaatcggtta aatcctgttc aatacaccat gtactgtgtt ttttgaaata atttttactg
     7381 ggataaaggt tgcgaagttt aacaattata gtgtcactct caattgtggc aattgttgct
     7441 ttcaaaaata tcttttttgt tgggtcatcg ctatgtaaaa ggttagagtc ttgtggataa
     7501 ataatacata catcaccttc tctaaaaacg gttgataggt acgggttgtt gaaaagattt
     7561 cttttaaaac aaactgttaa ttgctcaaga tttgaatttg cttcatcata ttctagatca
     7621 gcaaaaataa tattgtggtc ctgtttttca tttatatcta atttccataa agaagaaaat
     7681 ccataatact tattatattc aatgtcgtca gatttaccaa gtttattaat ccattgttct
     7741 ttggctataa attgcaaaaa gttattaata tatttttttt caagattatt ttcaagatta
     7801 ttgatagtat taataaaacc atcagcgaaa tcttttttaa atgatttata ttcagaaaca
     7861 tcatttttta taacttctac tatagaatcg attagtaatt caccttttgc taacaaatat
     7921 tcataatgaa caatacaatt tcgtgctttg actatatctt gtgtaatgtt aatgtttgga
     7981 ttattttcaa aatttttctt tatagatatt aagcaattat cctgcttact actatataat
     8041 accaaacttt cacccaccct atcaggatac atggagttaa gcataatatc atagcaaaaa
     8101 acctgaatat tatcattaat atatggagca tcattttttg gagggttttt tgttgatttt
     8161 aattctatta tatctctaca aacagtagtg tcatctcgtt ggtattcaag caatatgtca
     8221 aacctaccac ttaatccata catatgcgat ataacataag gttcaatatg tattagattg
     8281 ttagattttg ttttagaagt ttttatattt tttacttttt ctgaatttac aaatgtaata
     8341 attgaattaa aatgtttttc tgagttttct tttaattttc ttatagtttc aggtagatct
     8401 tcagccttta cagaactcac aatgtcaaat gcatattccc tggtggcttt gttaaataga
     8461 tcatcaaatt ttgtatttcc tgtgaacacc agtgtgtcaa gaatgaaatt tgcaatgtta
     8521 ccctgaattt tttctatact tggtttaaat aattcaaatt tgttcaataa atatagtgaa
     8581 ggtattactt tattccatgt tacaaagcat tcttttattt gtgtgatgtt tattaaataa
     8641 tcaggttcaa taataaaaaa actttgtgga tttacactat aaaatggttt gtcaggtgtt
     8701 ttaaaaatat taaatatatt tattgttgcg ttttcccaca gaacttcttt cagatgtatg
     8761 aacaatgagt tatttttatt aatgtatata tgcatataat ttaaattgtg ctggtcaata
     8821 catgtaatat ataattgatt attttctttt ttaattttat atatagaagc ctgcacaaat
     8881 tctatttttt cttttggtat ctctggttta tcaggtaatt caagatgtgc aacagattga
     8941 tatatatcct tgagttttct tggtatatca catttactaa aataattaat tatctgacac
     9001 aatgttttta ataaaataag atatttttcg ttgctaacag aatcgggata accataatga
     9061 tggaagggat ttaattgagc acgtagtgaa taacattttt ttctaatatc attatcagca
     9121 gtagatgttt taaaaacata ttccattcgt gctgcatgat ttattaattc actgtcaggc
     9181 atatttttag caagttcaga aaatagcttt tctaaaataa ggcgaaattc cttaacttta
     9241 tatatttttg tcttctcttc atctatagta tctttatttt ttatttcaat gctaactatt
     9301 tcttccagtt ttgaatataa atcaatagca cattcattat taatttgtat ggtattcaat
     9361 gacattgtag catccctgtg ttaaaatttt ttaaatagta gtatgtatta aatttgcctg
     9421 tcaatattat ttttagttat aactatagtt acttgtaaat attaaattaa ataattcaca
     9481 taattcccgt tatactaata ccatccattt ataaattctt taatatcctc ataatgcaag
     9541 ccaggtgtgt gtaaaaaaat ccttataaaa aataatcttt tgctgtctaa tctattataa
     9601 taatcctgca atgccaatca tggatatgcc caacacctac taacagtaag aaagaaaact
     9661 attaatgcaa aagggaggtt gcattatgct tactgtatat gtgtatgaac agggagcaaa
     9721 gattactaag gagggtgaag tacttaccgt aaacatgccc gatggaacca cccagaatgt
     9781 gcgcattaac aatattgata gaataatcat ttatggaaat gtgcaattta caactcaggc
     9841 actcacattg ttgctatggg acaatattcc tgtgtctttt ttaacgctaa atggtgagta
     9901 taagggtagc cttgtaactg ccgatggtgc caattgtttc aggagaatta agcaatatga
     9961 aatactaaat aataaagaaa aaagattggc tttggctcgc cgtatagtgt atgcaaagct
    10021 ttataatgga tatgtattca taagaaacta ccaccatcat aagacattgg aaaattttgg
    10081 taagtataac catattattg aaaatgtaat acagcggtgc aagatagctg caacaataca
    10141 ggaattaatg ggttatgaag gctttggaac caaaagctat tttgcaatgt acacagcatt
    10201 atttgataag caatggaatt ttaccaaacg tacaaaacgc ccgccgatgg atcctgtcaa
    10261 tgctatgatg tcattgggat atacacttgt ttcaaccgaa attgcttcgt tactggaatc
    10321 gcatggcctt gatacatcgt taggtatgta tcattcactt gaatatgggc gacagtcatt
    10381 ggcactggat attgttgagg aattcagggc tgtgcttgtg gatagatttg tgtttcagtt
    10441 actatcgcaa aaaatattta aacctgatga ttttgaatat gatgaacaaa aagggtgcag
    10501 atttaaaaaa gaagcgttaa aggagtattt ggtacggtac aataagctat taaacacaga
    10561 tctgggatta aaagaaccag ttacgtggcg acagttacta tggcgacagg tggaaagaat
    10621 ggttgcattc attgataagg atgttgaata tatgcctctt ttgtatggag aagtatatga
    10681 gagtagtagt ggtatatgat gtacgggaca atagacgcag aaagcgattg cacacattgc
    10741 taaaaggata tggccagtgg caacaatatt cagtatttga gtgcgagtta agccaaaaga
    10801 aatattttga attggtcagc agggtaaaat atataattga tgaatctgaa gattcggtat
    10861 tgttttataa attatgcgca atgtgtgtaa cgcaaacaca atccataggt acggccattc
    10921 tttatgaaga ggaagaagta ctggtaatat aaatttattg aagttttaac aaacacagta
    10981 aactatacaa aggtgacagg gattaaataa aaaataaaaa acagcatata aaaaaatgat
    11041 tgacaaaaat gaatatagtg gattaagtaa atatactata acaagtagta ttacagaata
    11101 taataatact attatgttta attattgaaa tttaagaaga cactataaca taagtgttgt
    11161 ttactaatag gtctttgaaa acaaaaaacg cataaatggc taaagattgt tgttggctat
    11221 agggcaacac tttattaaaa gtgaactttt acgcaaaaaa atttagtcaa tttctgaacc
    11281 tcgtgattgc ttgtaaatgc tgaggaattc aggagttata attggacgat gtccaaaagg
    11341 gataaaaaaa tggcattatg tctaggaaat taatgaacct cgtgattttt gcctttgtaa
    11401 agcctggaaa atccagcagt tacaaaggta agggtccgaa aggatgcccc gctaataggg
    11461 gattgcgac
//
LOCUS       DAORYL010000094        10606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_4717_length_10606_cov_12.255805, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000094 DAORYL010000000
VERSION     DAORYL010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10606
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4717_length_10606_cov_12.255805"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1622
                     /locus_tag="PLV81_13330"
     CDS             <1..1622
                     /locus_tag="PLV81_13330"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HOM11120.1"
                     /translation="TAQHNQQKHIWWKHGVIYHIYPRSFYDANDDGIGDLPGIIEKFD
                     YLIDLGIDAIWLSPIYVSPMYDWGYDIRDYYNIDPCFGTLDDFKKLLAIAHSNNIKII
                     MDMVLNHTSHLHPWFIESFSSLDNPKRNWYIWHDPVNGKTPNNWKSAYLSSAWQWHEP
                     TQQFYLHSFLKEQPDLNWHNPEVKEEMFNVMRYWLDIGVDGFRLDVINYCYKDNRLRN
                     NPFSLNPFNEQIEKYNRNRPENYTLVQEIRQRIDSYNDRMVVGEVFSYPPGNPELSAS
                     YLCNGKGLNLAFDFSLLYQPLDARKIYKSIKRWYNAIPQNCWPCNVLSNHDQPRYADK
                     HNSDNTDAVQRLLALMLLTLRGTPFIYYGEEIGMKNCSIPLRHIKDPAGKRFWPIYRG
                     RDCSRAPMQWSDEPNAGFSRSCSWLPIDSNYRTINVKNQIKDRYSLLNFFKDCIHLRK
                     KHTALTYGLWEPLEKGKHGIFAYLCKTDQETLCIILNFNNNPTMLNHRYEAQWKVLYS
                     THRSKFEHFADLRMQCYPYEATILQKVGDLS"
     gene            complement(1624..2394)
                     /locus_tag="PLV81_13335"
     CDS             complement(1624..2394)
                     /locus_tag="PLV81_13335"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOM11121.1"
                     /translation="MEIQWFGTASIRISSGNTSLHFDPFISMNKSLQYASIDDYSGAT
                     DIFITHGHFDHIYNIPEIWDLSKCTIYCGIQVADVLLKQGVLPDYIRIIDHGQVVLIP
                     PFIIKIYKSEHIRFDIPLIFKTLINKRVLQYFGDFKQIITLNKTMPAGTIFAYEIVCD
                     DKSILHFGSLNYSPTIEYPYKPAMLTLPFQGRSDIDHYAIRFIKKFNPQSIFLHHFDD
                     TFPPVSSHVDTTQFVEAVTKAFPHITVIIPQYGIPYTL"
     gene            2632..3111
                     /locus_tag="PLV81_13340"
     CDS             2632..3111
                     /locus_tag="PLV81_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11122.1"
                     /translation="MNRLLIVIFILLFTLHHSFANESLNCITSHTSALGFKADLPDWD
                     KSEMMRGISHEFICAYSKTVYIKIYKVKDSQTDMLHELTWNIWEKDPNAHFIIKTSTE
                     DGGIIVTTYRSQGTSIHRIRLIHNNDTVFIIECSAPEKAFYKYEVYFNRVFQSFSVL"
     gene            complement(3100..3999)
                     /locus_tag="PLV81_13345"
     CDS             complement(3100..3999)
                     /locus_tag="PLV81_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOM11123.1"
                     /translation="MKAILKPSTIAIFKKHGWRVDRAIHNYLYFVFYYPYVFILYHTF
                     KFLTDHFSWFTPLRHFLKFAFDRYHAKILSFGDTRKILELNRDIAHTSDSNRSIIPYK
                     FAYKIIFTEPDFIAVMDCPCKKTMGDEEWAIHSCIAIGKPVASFWLEHGKKYNAERIT
                     QHQALELIKNFRKHGYITQAFFKVATGGSTGVICNCHPKSCVSLKATQFAKKFDDSLS
                     MNAAAGYSVVHDETKCKHCGDCSRICPFNAISVSNTSWMYNKNDCAGCELCVEHCTHN
                     ALSMDIDKSKPRPLDIAALPLKY"
     gene            complement(4020..>8412)
                     /locus_tag="PLV81_13350"
     CDS             complement(4020..>8412)
                     /locus_tag="PLV81_13350"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOM11124.1"
                     /translation="PQAFISIIRDISGRIRAEEELKEREAKYRTLFESANDAIFLMDK
                     EIFIDCNQKTLEMFGCTREQIIGTPPYVFSPEFQPDGRSSKEKALEKINAALQGNPQF
                     FEWQHCTYDRTPFDTEVNLNAFELGGKQYIQAIVRDITERKKAEKALLLSEQQYRILV
                     ETAKEGIWKIEHNITTYVNKAMADMLGYTQEEMIGRSIFDFMFEEDIPVLQKRLQEKR
                     AKGIDEVYEDRRKKKDGSELWAIVSAKAIIDEEGKYIGSFALYTDITERKKAEEELFN
                     KQKQLEDIIAYIPDPTLVIDKNGVVIACNKAMGELLGINASDLIGKGNYEYALPLYGV
                     RRPILVDLALQYNEDVAKNYTYVKKEKDTIVAETTIDDFKGKKVYLWGKAAPLYDKDG
                     NIIGAIETIRDITERKLNELALKESEQKFREIFDSTTDAIMIDAITEEGGRIIDCNRQ
                     TLEMYGYSSKEEILHLNIGDLSANVYPYTEDIAQQKIAEAIRGEIPTFEWLAKKKNGQ
                     VFWVEVTLKKTILAGQERILAIIRDIDERKKAEKELKESEERYRYLAESTQDLIALHD
                     MDGIIQYVNQAVINISGYTQEEIIGNNILMFVPRKYRRSIYERHAKRLSGYSGIELYE
                     TEFINKFGTLIPVQVTSTPIVKDGKIISIMIVAHDLTQRKIAEELLIKNKERLDAAIA
                     ILQYDAKNSKELFEYVLEKALQLTGSNIGFILNYDADEKKFVLNAWSKEVMHECTMIE
                     KPEEYYLDTAGIWAETVRQARPIIINNYKEDNPLKKGYPEGHVDIDRFLGIPVFRNKE
                     IVATVGVANKETDYTEMDVLQLTLLMDAAWKSYERMQVTGALAESEEKFRTLADSSPM
                     AIMMYQDDTFVYVNKVAEQISGYSFEEVAHLNFWFLVHPEDKEMLINKAQRRQQGGQG
                     LDSYEFRIISKDGQVKWVYVTSATVQYKGKPAGLVSVLDITDRKKAEEALFEEKEKLR
                     ITLQSIGDGVIATDTDGRVVIINETAQQLTGYSQVEATGKHLSEIFTIVHELSAKPLE
                     NPVDKVLSTGHVYELSNHTVLVAKDGTRRVIADSAAPIKDKAGNIVGVVLVFRDMTEK
                     LQLIEQIQRAQKLESLGLLAGGIAHDFNNILEGVFGYIGLASAYVSDSTVSEMLANAL
                     KSIQRAKGLTGQLLTFSKGGQPVKKVQSIIPLLQDTVQFAISGSNVKAQFSVDDNLDY
                     AEIDYNQISQVIQNIVINAVQAMPMGGIITIGAGNISFAEGEHPLLQGDYIRVTIADQ
                     GIGIPKEVLPKIFDPFFTTKSMGQGLGLATSYSIIARHQGTIEVESEPGKGTTFYIYI
                     PATKKEPAEPKKVEERKVSKGGRILVLDDEPMMREIMLKFLEHLGFNAVAVDDGRDAI
                     NMYIKEKESGTPFNALIFDMTIKGGMGGREAIEEIRKYDKDILAFVMSGYHEDPVLAH
                     PQDYGFNGGLCKPFTLEELEAMLARYF"
     assembly_gap    8413..8512
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8513..10111)
                     /locus_tag="PLV81_13355"
     CDS             complement(<8513..10111)
                     /locus_tag="PLV81_13355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOM11125.1"
                     /translation="MNNKKKEKTAKKKVTPSQTHNIVHAESLNPLYNMDIFSIIANVS
                     ADAITIFDMNFNIVYVSPAIVTLRGYPLNEVKKQKLEQILTPDSLKKALEVFKEELEK
                     EKSKNVNPNRSRILELEHYRKGGSTIWLETQVSFIRNTKNKPIGILVISRDITKRKAI
                     EDQLFLFKYLVENSSYAIGMSTPEGKHYYQNKAFDELFGDIGNNPPRTLYVDHKIGKE
                     VFNTIMAGGSWNGQVKMYDKNKNVIDVLLRANAIKDKDGNIIGLVGIHEDITTIKLME
                     KILKENEEKFKAIFESTTDAVFIMKDDICIECNPATLTMFNCTRSDIIGKSAYQFHPV
                     LQPDGEKSKPLALQYIKKAKAGIPQHFEWQHIKTDGTPFYTEVSLNKLVISKEVFLLA
                     IVRDITHRKEIENRLKKSEEWYRALFDTSPDVIVIYDFKGNIFAANHAAREMYGVGTT
                     GELMSDLTNILDLLDEEDKRKAITNMSNTLKTGYSEGNIYTIKNKNGQKITVEVHSSV
                     LPDSEGKPQAFISIIRDISGRIRAE"
     gene            10308..>10606
                     /locus_tag="PLV81_13360"
     CDS             10308..>10606
                     /locus_tag="PLV81_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11126.1"
                     /translation="MAPTTARKFTQQQRAEILSLIDERIKDQYVTRSDFKVLQKAITD
                     LAITVKELAEAQKRTEQSIKELTKKQDSSDRNIKELTQAQKRTEQSIKELTERQN"
BASE COUNT         3191 a         2116 c         1648 g         3551 t
ORIGIN      
        1 taacagcgca gcataatcaa caaaagcata tatggtggaa gcacggggta atatatcaca
       61 tatatcctcg cagcttttat gatgcaaatg atgatggcat aggggacctg cctggcatta
      121 ttgagaaatt tgactacctt attgacctgg gcattgatgc tatatggtta tcgcccatat
      181 acgtttcacc aatgtatgat tggggttatg atatacgtga ttattacaac attgacccct
      241 gctttggtac attggatgat tttaaaaaat tattggcgat agctcatagt aacaacataa
      301 agattataat ggatatggta ttaaaccata cctcacacct tcacccgtgg tttattgaat
      361 ctttctcttc gcttgataat cctaagcgca actggtatat atggcatgat cctgtaaacg
      421 ggaaaacacc aaataactgg aaatcagcct atttgtcaag tgcatggcaa tggcatgagc
      481 ctacccaaca gttttattta cattcgtttc ttaaagaaca acctgatctt aactggcata
      541 atcctgaagt taaagaagaa atgttcaatg ttatgcgcta ctggcttgac ataggtgttg
      601 atggattcag attggatgtt attaattact gttataagga taatcgcctg cgtaacaatc
      661 ctttttcact taaccctttc aatgaacaga tagaaaaata taatagaaac aggcccgaaa
      721 attatacact ggtacaggaa attagacaac gtattgattc atataatgac agaatggttg
      781 ttggtgaagt ttttagctat cctcctggta atcctgaatt atccgcaagc tatctttgca
      841 atggtaaagg gttgaattta gcttttgatt tttcactatt atatcagcca ttagatgcac
      901 gtaaaattta taagtccatc aaacgctggt acaatgctat ccctcaaaat tgctggccat
      961 gtaatgttct ttcaaatcat gatcagccac ggtatgccga taaacataac agtgacaata
     1021 ctgatgcagt ccaacgttta ctggcattaa tgcttctgac tttacgcggt actccattca
     1081 tttattatgg tgaggaaatt ggcatgaaaa attgttcaat tccacttcgc catattaaag
     1141 atcctgcagg aaaaagattc tggcctatat acaggggaag agattgttct cgtgcgccaa
     1201 tgcagtggtc agatgaacca aatgctggtt tcagcagaag ctgtagctgg cttcccattg
     1261 acagtaacta tcgcacaata aatgtaaaaa atcagataaa agaccgttac tcactgctta
     1321 acttttttaa ggattgtatc caccttcgca aaaaacatac agcactaacc tatgggttat
     1381 gggaaccact tgaaaaagga aaacatggca tttttgcata cctgtgcaaa accgatcaag
     1441 aaacactgtg tataatttta aattttaaca ataacccaac aatgctaaac catcgctatg
     1501 aagcgcagtg gaaagtactg tattccactc ataggagtaa atttgaacac tttgctgacc
     1561 tgcgaatgca atgttacccc tatgaagcaa ctattctaca gaaagttggc gatttatcat
     1621 agtttataat gtatagggaa tgccatactg ggggataata acggtaatat gcgggaaagc
     1681 ctttgtaact gcttcaacaa attgtgtggt atccacatgt gatgacactg gtgggaatgt
     1741 atcatcaaaa tgatgtaaaa atattgattg aggattaaat ttctttataa agcgtatggc
     1801 atagtgatcg atatccgaac gcccctggaa cggaagtgtc agcatggctg gcttataagg
     1861 atactcgata gtaggggaat aatttaaact gccaaaatgt agtatagatt tatcatcaca
     1921 tactatctca taagcaaata ttgtacctgc aggcattgtt ttattcagag ttataatttg
     1981 tttaaaatca ccaaaatatt gtaatacccg tttattgata agagttttga atataagagg
     2041 aatatcaaaa cgtatatgct cacttttgta tatttttata ataaagggcg ggattaacac
     2101 aacctgtcca tgatcaataa ttcgtatata atctggcaaa acaccctgtt taagcaaaac
     2161 atcggcaacc tgtatcccac aatatatggt gcatttactc agatcccaaa tttcaggtat
     2221 gttatatata tgatcaaaat ggccgtgtgt aataaatatg tctgttgcac ctgaataatc
     2281 atcaatggaa gcatactgca atgatttatt catggatatg aatggatcaa agtgtaaaga
     2341 agtgttgcct gaagatattc taattgaagc tgttccaaac cactgtattt ccatatgtgt
     2401 atctatctaa ataaaatcat ccacagttaa cattctttat cttcacctgt caagtacaat
     2461 tgatatagat tcagtaatat aaagaactat ttttaaaaat aggttattat ttaacattat
     2521 tttttaaatt aaccagtatt atcaatctat taccaaaaaa attgttgcaa attcatccat
     2581 aattatctat ttctaagacg ctataaaaac tcttaaaaaa gaggacaatc catgaatagg
     2641 ttattgatcg ttatatttat tttactattt acattgcatc attcttttgc aaatgaatca
     2701 ttgaattgta tcacttccca tacaagcgcc ttaggtttta aagcagacct tcctgactgg
     2761 gacaaatcag aaatgatgag gggtatttcc catgaattta tttgtgctta ttcaaaaaca
     2821 gtgtatatca aaatatataa agttaaagac agccaaactg acatgctcca tgaacttacg
     2881 tggaatattt gggaaaaaga tccaaacgct cactttatta tcaagacatc aacagaagat
     2941 ggtggaatta ttgttacaac atatagaagt caaggcacat ccattcacag gataaggctc
     3001 atacataata atgatacagt ttttattatt gagtgcagtg cacctgagaa agcattctac
     3061 aaatatgaag tttattttaa cagggttttc cagagttttt cagtacttta atggcaatgc
     3121 tgcaatatct aaaggacgtg gttttgattt atcaatatcc atcgataatg cgttatgtgt
     3181 gcagtgttcc acacagagtt cacatccagc acaatcattt ttattataca tccatgaagt
     3241 gttacttaca ctgattgcat tgaaagggca tattcttgaa caatccccgc aatgcttgca
     3301 ttttgtttca tcgtgtacta cactataccc tgccgcggca ttcattgata atgaatcatc
     3361 aaacttttta gcaaactgtg ttgcctttaa gctgacacag gattttggat gacagttgca
     3421 tataactccc gtactgccac cagtagcaac cttaaaaaag gcctgtgtaa tatatccatg
     3481 cttcctaaaa tttttaataa gttcaagtgc ctgatgttgg gttattcttt ctgcattata
     3541 tttttttcca tgttcaagcc agaacgatgc aacaggttta ccaatagcaa tacaggaatg
     3601 tattgcccat tcttcatcac ccatagtttt tttacagggg caatccatca ctgcaataaa
     3661 atcaggctca gtaaagatga tcttatacgc aaatttgtaa gggataatgc ttctattgga
     3721 atcactagta tgtgcaatat ccctgtttaa ttctaaaatt tttctggtat caccaaatga
     3781 taatatcttg gcatgatacc gatcaaacgc aaatttaaga aaatgtcgca gtggtgtaaa
     3841 ccaggaaaaa tgatcagtca aaaatttaaa tgtatgataa agtataaaca cataaggata
     3901 atagaagaca aaatacaaat aattatgaat agccctgtca acacgccacc catgcttttt
     3961 gaatatagct attgttgaag gtttgagtat tgctttcaat gttcctctta catactatat
     4021 caaaaatacc ttgctaacat tgcctctaat tcttctaacg taaaaggctt acataatcca
     4081 ccgttaaagc cataatcctg gggatgtgcc agcaccggat cttcatgata accgctcatg
     4141 acaaatgcca gaatatcttt atcatacttt ctaatctctt caatagcctc tctgccaccc
     4201 atcccgcctt taatggtcat atcaaagatt aaagcattaa atggtgttcc tgattctttt
     4261 tcttttatat acatatttat tgcatccctg ccatcatcca ccgctacagc attaaaaccc
     4321 agatgctcca gaaattttag cataatttcg cgcatcatag gttcatcatc cagcaccaga
     4381 atcctgccac cttttgatac tttcctttct tcaactttct ttggttctgc tggttctttt
     4441 tttgttgcgg gaatataaat ataaaacgtt gtacctttgc caggctctga ttcaacttca
     4501 atggtaccct gatgacgtgc aataattgaa tagcttgtag caagtcctaa tccctgtccc
     4561 atacttttgg tagtaaagaa aggatcaaaa atttttggca aaacctcttt aggaatacca
     4621 ataccctgat cggcgatagt taccctgata taatctccct gcaataatgg atgctcccct
     4681 tcagcaaagg atatattgcc tgcacctatt gtgatgattc cccccattgg cattgcctgc
     4741 acagcattga tgacaatatt ttgaattacc tgtgaaattt gattataatc aatttcagca
     4801 taatcaagat tatcatcaac agaaaactgt gctttgacat tagaaccgct tattgcaaac
     4861 tgcactgtat cctgaagaag cggaataata gattgaacct tcttaacggg ctggccgcct
     4921 tttgaaaatg tcaacaactg ccctgttaac ccttttgcac gttgaattga cttcagcgca
     4981 tttgccagca tctcagaaac agtgctatca gatacatatg cacttgccag cccaatgtat
     5041 ccaaatactc cttcaagtat gttattgaaa tcatgtgcaa tcccacctgc cagaaggcca
     5101 agggattcta atttctgtgc tctctgtatt tgttcaatca actgcagttt ttcagtcata
     5161 tccctgaata ccagtaccac acctacaata ttgcccgctt tatccttaat tggtgcagca
     5221 ctgtcagcaa tgacacgtcg cgtgccatct tttgccacaa gtacagtatg atttgacagt
     5281 tcatatacat gcccagtaga taacactttg tcaacggggt tctctaatgg tttggccgat
     5341 agttcatgga caattgtaaa aatttccgaa aggtgcttgc ctgtagcttc tacctgtgaa
     5401 tatccagtaa gctgttgtgc tgtttcattg atgattacca cccttccatc agtatccgtg
     5461 gcaataacac catcaccaat actttgcaaa gtaatacgca acttttcttt ttcttcaaat
     5521 agtgcttctt ctgccttttt acgatcagtt atgtcaagta ctgaaacaag accagcaggc
     5581 ttacccttat attgtactgt tgcacttgta acataaaccc atttaacctg cccatcttta
     5641 ctgataatcc tgaattcata gctatctaac ccctgtccac cctgctgccg ccgttgtgct
     5701 ttattaatga gcatttcttt atcttcagga tgtaccagaa accagaaatt caaatgagcc
     5761 acttcttcaa aactatagcc tgaaatttgc tcagccactt tattcacata tacaaatgta
     5821 tcatcctgat acatcataat agccataggg ctggaatcgg caagtgtcct gaacttttct
     5881 tcgctttcag caagtgcacc tgtcacctgc atacgttcat acgacttcca tgcagcatcc
     5941 attaacagcg ttaactgcaa tacatccatt tcagtataat ctgtttcttt gtttgccaca
     6001 cctaccgtgg caactatctc cttattcctg aacaccggta tccctaaaaa tctgtcaatg
     6061 tccacatgac cttcaggata acctttttta agcggattat cttctttata attatttatt
     6121 attattggcc gtgcctgtcg aactgtttcc gcccatatcc cagctgtatc taaataatac
     6181 tcttcaggtt tttcaatcat ggtgcattca tgcatcactt cttttgacca ggcgttaagc
     6241 acaaatttct tttcatcagc atcataattt aaaataaatc caatgttact gccagtaagc
     6301 tgcaaggctt tttccagcac atactcaaaa agttctttag aattttttgc atcgtattgc
     6361 agtattgcaa ttgctgcatc caatcgctcc ttatttttta tcagcagttc ctctgcaatc
     6421 tttcgctgag ttaagtcatg agcaacaatc ataattgata taatcttgcc atctttgact
     6481 ataggggttg atgtaacctg tactggaatc aacgtgccaa atttattaat aaattctgtt
     6541 tcatagagtt caatacctga atatccggac aatcgctttg catggcgttc atatattgaa
     6601 cgcctgtatt tacgaggtac aaacatcaat atattattgc caattatttc ttcttgtgta
     6661 tatcccgaaa tattaatgac tgcttgatta acgtattgta taataccatc catatcatga
     6721 agggcaatta aatcctgtgt tgattcagct aaataccggt aacgttcttc gctttctttt
     6781 aattcttttt cagctttttt tctttcatca atatcacgaa taattgctaa tatcctttcc
     6841 tgtcctgcta atattgtctt tttcaatgtt acctcaaccc agaacacctg accattcttc
     6901 ttttttgcaa gccattcaaa tgtggggatt tctcctctta tggcttcagc aattttctgc
     6961 tgtgcaatat cctcagtata cggatataca tttgcactca aatcacctat gttcaaatgt
     7021 aaaatttctt cctttgaaga gtatccatac atttctaatg tttgtctatt gcaatcaatt
     7081 attctaccac cttcttcggt gattgcatca atcattatag catcagtggt agaatcaaat
     7141 atctctcgaa atttttgttc gctttctttg agtgcaagtt cattcagttt tcgttcagtt
     7201 atatcgcgta ttgtctctat agcacctata atgttaccat ctttatcata cagtggtgca
     7261 gccttacccc ataaataaac ctttttacct ttaaaatcgt ctatagttgt ttcagcaact
     7321 attgtatctt tttctttttt aacatatgta tagtttttag caacatcttc attatattgc
     7381 aaagccagat ccaccagtat tggtcttcgt actccataca atggaagtgc atattcataa
     7441 ttacctttac caattaaatc agatgcattt attcctaata attcccccat tgctttatta
     7501 caggctataa caactccatt tttatctata actaaagttg gatcaggtat ataagcaatg
     7561 atatcttcta actgtttttg tttattgaat aactcttcct cagccttttt tctttcagtt
     7621 atatcagtat ataatgcaaa tgatcctata tattttcctt cttcatcaat gattgcttta
     7681 gctgaaacta tcgcccacag ttcactcccg tcttttttct tccttctgtc ttcgtatacc
     7741 tcatctatac cttttgcacg tttttcttgt aaacgttttt gtaaaaccgg gatgtcttct
     7801 tcaaacataa aatcaaaaat tgatctacca atcatttcct cctgagtata tcccagcata
     7861 tcagccattg ctttattgac gtatgttgta atattatgtt caatcttcca tattccttcc
     7921 tttgctgttt caaccagaat gcgatattgt tgttcactaa gaagcaaagc tttttcagcc
     7981 ttttttcttt cagtaatatc acgcactatt gcctgaatat actgtttacc acccagttca
     8041 aaggcattca ggttcacttc agtatcaaat ggtgtcctgt catatgtgca atgctgccac
     8101 tcaaaaaact gcggattgcc ctgtaaagct gcattaatct tttccagtgc tttttccttt
     8161 gaggatctcc catcaggttg aaattcaggt gaaaaaacat acggtggtgt gccaattatc
     8221 tgctcccgtg tgcatccaaa catttccagt gttttctgat tgcaatctat aaatatttct
     8281 ttatccatta aaaaaatagc atcatttgct gattcaaaca aagtacgata ctttgcttct
     8341 ctttctttta attcttcctc tgcccttata cgcccgctaa tatctctgat tatgcttata
     8401 aatgcctgag gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnctctgccc
     8521 ttatacgccc gctaatatct ctgattatgc ttataaatgc ctgaggttta ccttcactat
     8581 cgggaaggac tgatgaatgg acttcaacag tgattttttg gccattcttg ttttttattg
     8641 tataaatatt gccttcagaa tacccggttt taagggtatt actcatattt gtaattgctt
     8701 tacgtttatc ctcttcgtcc agcaaatcca gaatattggt aagatcagac atcaattcac
     8761 cagttgtacc aacgccatac atttcccttg ctgcatgatt tgcggcaaat atattacctt
     8821 taaaatcata aatcacaatg acatcgggtg aagtatcaaa tagtgccctg taccattctt
     8881 cacttttttt tagtcggttt tctatttctt tccggtgagt aatatccctc actattgcca
     8941 gtaaaaaaac ttccttactt attaccagct tatttaaact tacttcagta taaaaaggcg
     9001 tgccatctgt ttttatatgt tgccattcaa aatgctgcgg aatacctgct tttgcttttt
     9061 tgatatattg taatgccagt ggctttgact tttccccatc aggctgcaag acagggtgaa
     9121 actgatatgc tgatttgcct ataatatccg agcgtgtaca gttaaacatg gtcagtgttg
     9181 cggggttgca ttcaatacat atatcatctt tcatgataaa aacagcatcg gttgttgact
     9241 caaagattgc tttgaatttt tcctcgtttt ctttcagtat cttttccatt aacttgattg
     9301 tggtgatatc ctcatgaata ccaacaagtc ctattatatt cccatcttta tccttaatgg
     9361 cattagcacg caaaagaaca tctataacgt ttttattttt atcatacatt tttacctggc
     9421 cattccatga accaccagcc ataattgtgt taaacacttc tttcccaatc ttatgatcaa
     9481 catataacgt cctgggtgga ttattaccaa tatcaccaaa cagttcatca aatgctttgt
     9541 tctggtagta gtgcttaccc tcaggtgtgg acatgcctat ggcatacgaa gaattttcaa
     9601 ccagatattt gaaaagaaat aattgatcct caattgcttt ccttttggta atatcccggg
     9661 atataaccag tatccctatt ggcttatttt tagtattacg aataaatgaa acctgtgttt
     9721 ccagccatat tgttgatcca cctttgcgat aatgttccag ttccaggatc ctgcttctat
     9781 ttggattaac gtttttactt ttttcttttt caagttcttc cttaaatacc tcaagtgcct
     9841 tttttaaaga atctggtgtc aaaatctgtt caagtttttg tttttttact tcattcagag
     9901 gataaccacg taatgtaaca atagccggac ttacataaac aatattaaaa ttcatgtcaa
     9961 atatggtgat agcatcggca ctgacattgg caataatact gaatatgtcc atattataga
    10021 gcggatttaa tgattcggca tgaacaatgt tatgagtctg ggatggcgtt acttttttct
    10081 tagcagtttt ttcttttttt ttattattca ttataattcc tcagttatca ataaaaccag
    10141 ccttcctttt ataacaataa tcgataacgt tggaaaatac aactaaaatt tttgctgtaa
    10201 aaattctgaa aaggaagccc aagataataa aatttttgtt gatttaaaat aaaagaatat
    10261 ctatattagt gtttagtaaa ttattgaaca tatcttaagg tacattaatg gctccaacaa
    10321 cagctcgaaa atttactcag cagcaacgtg ctgaaattct atcgctaata gatgaacgta
    10381 ttaaagatca atatgttaca cgttctgatt ttaaagtatt acaaaaagca ataacagatt
    10441 tagccattac cgtcaaagaa ttagctgaag ctcaaaaacg tactgaacaa agtatcaaag
    10501 aacttactaa aaaacaggat agttctgatc gtaacattaa agaactcact caagctcaaa
    10561 aacgtactga gcaaagcatt aaagaattaa ctgaaagaca aaataa
//
LOCUS       DAORYL010000095        10333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_4865_length_10333_cov_17.066258, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000095 DAORYL010000000
VERSION     DAORYL010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10333
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4865_length_10333_cov_17.066258"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            429..2159
                     /locus_tag="PLV81_13365"
     CDS             429..2159
                     /locus_tag="PLV81_13365"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOM11127.1"
                     /translation="MRMSRYLVPTLKEDPADAVITSHKLMIRAGLIRKESAGMYIYLP
                     LGLRVLQKIITIIREEMNKAGALEFLMPELTNADLWKESGRWHTMGPEMIRIKDRNGL
                     EYALAPTHEEAFTNAIRMIVTSYRDLPVNAYQINTKFRDEIRPRFGVMRSKEFIMKDA
                     YSFDIDEAGLERSYQTMRQTYRNIFKRCGLETIPVEADTGSMGGSDSEEFMVASEVGE
                     EVLLLCDACGYKSNQERATCQYELSPQNENVEALSIVDTPNVRTIDELVQFFGCSAHK
                     FLKSLIYLADGNPVMAVLPGDRDINEVKLKNVLGAVEVALAPDKVVEEVTGAPVGFAG
                     PILREGKTIRIIFDTQVKHIVNGITGANEKDKHYKGVNPGRDFVIKEEADITRVVAGD
                     KCPHCGYLLKEKKGIEVGHIFKLGYKYTKSMNVTVLDQNGKAITPIMGCYGIGVNRTM
                     AAVIEQHHDERGIIWPMSVAPFHVHCVSLAKNEAEEAMADEVYSILLKAGIEVLYDDR
                     KASPGFKFGDADLIGIPIRITLGKAFFGDSSSIEVKLRNQDEKVIIAKDSIVTYCLNL
                     IAESLKTLES"
     gene            2192..2341
                     /locus_tag="PLV81_13370"
     CDS             2192..2341
                     /locus_tag="PLV81_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11128.1"
                     /translation="MGEAITVSILVILALVYSIQYVVAKFTKPQQCDCTQCPIADCKE
                     RKRTV"
     gene            complement(2512..2865)
                     /locus_tag="PLV81_13375"
     CDS             complement(2512..2865)
                     /locus_tag="PLV81_13375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOM11129.1"
                     /translation="MSKPIDLEGRVTHDPILRKTKTGKSVCIFTIAMDHPVKGETKPH
                     VSFIDIETWEKLADLCAENIRKGKKIQATGRLKQDRWQDDTGKPRSKIKLVSNQVIFI
                     EPLNSKHNEEVAIES"
     gene            3138..4547
                     /locus_tag="PLV81_13380"
     CDS             3138..4547
                     /locus_tag="PLV81_13380"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008083070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOM11130.1"
                     /translation="MAEEKKSPNQELQEKISVKKESAWAQLKDKVDSIYSFGEEYKLF
                     IGKNKTERLCIRTMVEMLEKAQFSSINTKGTLNPGDAVYKIIKGKSLLACKVGSDPMK
                     VRIIGAHVDSPRLDLKPYPLYEDAELALLKSHYYGGIKKYQWVNIPLAIHGVAFTKEG
                     KTVEIHIGENPDEPRFIIPDLLPHLAKDQMKKTGETIIEGEDLNVIFAHIPVDDKDIK
                     EKIKFNVLKYLHSTYSLIEEDFVAADIELVPALQPVDIGIDRGLIAAYGQDDRACAYT
                     AFRALLDAQDCKETVLLYLADREEIGSMGDTGAESFVFVNFMREYIRKTGITATWDEL
                     LENALSISADVTGALDPTFKSVNDPNNVSYLGHGVSIEKYGGRGGKYSTSEAHAEYMH
                     YLRSLATKHGIPWQTGEQGKIDIGGGGTIAMFMSRYGMDCVDVGPCMLGMHSPFEVTS
                     KVDIYCTYLLYKAFFEDAR"
     gene            complement(4562..5737)
                     /locus_tag="PLV81_13385"
     CDS             complement(4562..5737)
                     /locus_tag="PLV81_13385"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM11131.1"
                     /translation="MWIERKIESLVYQAFEQFPALLITGLRQVGKTSLLRKMFPDASY
                     ITLDLPANQLAAASNSLEFIQRFARPVIIDEVQYVPQLFQGLKVAIDQQRRNSQFILT
                     GSQNFSFMQGVSESLSGRCAMLQLYPLSFYEVSNHTSISQNEFIFLGGYPELHTSPKN
                     YNLWYSSYVATYLERDVRNVLNVTDLREFNLFLRSCALRISNLLSYTDLARDIGISVN
                     TAKKWLSVLTTLGAVYLVEPYFANRGKRIIKSPKIYFTDTGLAAFLCGFEHAQQLHNS
                     SMAGYFFENYIANEITKHYSFYGKRLNLYYWQDIHGKEVDFIIEHASGKIIAIECKLT
                     ENPTIKDCNNIFKLIDYYGEDRVSTSYIVCNTPTPYQIDKKIYATNIGELLSTLLKQ"
     gene            complement(5778..6113)
                     /locus_tag="PLV81_13390"
     CDS             complement(5778..6113)
                     /locus_tag="PLV81_13390"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HOM11132.1"
                     /translation="MSDIEDIKQKAYELANSIAEHPVATRYFSLLQQMNEDEEALQLL
                     SRLVEHGKKLNQKMESGEIDIENDPVSVELREALIEKPLVKEFVSAQNEYLGLIKEIM
                     EILHHRLNP"
     gene            complement(6113..6976)
                     /locus_tag="PLV81_13395"
     CDS             complement(6113..6976)
                     /locus_tag="PLV81_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012161504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOM11133.1"
                     /translation="MKRFFLLSIVLLTAGMMCCSHQSQAQCTLAPGLHTIALPDAVIN
                     PGKTIPIDIIISDSNCKKNILVLPGWNFSRKRWIEETSLRQEATKRGYNLILPEMSVT
                     IYESHYYPETKRKWAKTPGSQWIKEICIPELQKRGLLLSNQFNAVMGLSTGGRGAVLT
                     ALYNKGIFKAAASLSGDFDQTRMKHDKLMTAVYGPYEYYKNRWENVDNPFAMIAEWYI
                     PLYLGHGTRDTVVPYEQTKIFYEALKTAKPDITITFNDCACGHDFIYWNSEVVPILDF
                     FDTINKKAGNK"
     gene            complement(6973..8115)
                     /locus_tag="PLV81_13400"
     CDS             complement(6973..8115)
                     /locus_tag="PLV81_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11134.1"
                     /translation="MAKIKSVSLLFVISCIISCGLYNNSSARTQSPFAATPIDTKLVE
                     ATRFLAGKPLSPSSLLFPLSTDKRYLAYVDELRKSWARLQQKNLDEIAQWKKQNLPAR
                     YNKTIFYPFSGPDILNAIAFFPDGDEYIMFGLEPCGNIPDPHAVSKERVFSGLWGLRA
                     SLNEILNMNFFKTIDMQKEVSTNEFNSMISIIMWFLGQLDYEVINAHKIWIDDNSLIT
                     TTPSVMKQNKYIPGAEIFFKDNAGYIKRVRYFQINVIDNSLSYYTNFIPHLESYNRYT
                     TIIKSASYLMHNDNKFTKIRDLTLQHSDLILQDDSGVALKFFSPYQWKLTFFGVYTKP
                     VPLFAHRYQPELKEKFDTLSKGPLPFSYGYNSGIGNSNLMLAERVQ"
     gene            complement(8282..9610)
                     /gene="larA"
                     /locus_tag="PLV81_13405"
     CDS             complement(8282..9610)
                     /gene="larA"
                     /locus_tag="PLV81_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOM11135.1"
                     /translation="MKIDLPWGASYISLDLPASWDVIIPRQQEHKLSPKKQNEKDIVA
                     RALANPFSAKPIQKHVLKNKKVVIVVDDNTRPTPAYKFFDIVLDALKKAGCTTKHNAI
                     VIPALGIHTPMKKEEMEEKIGKKNIGQVQWENHDAFDKNKNAYFGKTSRGVEVYLNHH
                     LKDADFIILIGLVEPHLWAGFGGGLKNILPGVAYAETIGNHHAIIAEPPYRFNRVAVD
                     AEHNSFRLDLEETLNMIKAEVFCINVVLDSNKDIIACFAGHPIHCHREAVAFTKTICG
                     LPLHKQVDGIITNSAPMDINFKQSMKCVGNTLPALRPKGIVMGFLRAQRGLDDIPLPD
                     KSTPLWLTRTILRTLGPARVLGFLEKVKPGLDVEQKFLTYYSMQLIRAHNLYFYVPSL
                     SDTEVKALGFFERFNEPQEVIERGLKHLGKNATVAVFKEGGATFPLLSVK"
     gene            10060..>10333
                     /locus_tag="PLV81_13410"
     CDS             10060..>10333
                     /locus_tag="PLV81_13410"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOM11136.1"
                     /translation="MARKLRVQLPGLTYHITSRCIEWKSMLQEDYCKACFVEILNKAK
                     AKYHFKLIAFCIMDNHIHLVIHTLDDGAPISRIVQYIKARFAEVYNR"
BASE COUNT         3088 a         1857 c         2105 g         3283 t
ORIGIN      
        1 ttaaaaaaat tacattgatc ttacagtgct gatagagttt aacaagctgt attataccag
       61 cgattctaaa aatatgtcag ctgagatgat acggatgtta gaatgagtga tgatatctaa
      121 tcctaatttt tttacaacct gataacacta tggaatattt attctttcct tataatatag
      181 caggttattt gatgctgatg ttggtgcagg ttattataga ttggatttgt aagatatcgt
      241 tataatgtca tgctttacat ataaaaaagg ttgatgtaat gttaagaaat tttgccatca
      301 ggtaaaattt ttagagtatt ttgttgccta caggcaaaaa tgttagatag ggtaagtaaa
      361 tttgcacttg acacgctact gccattataa cacttatcaa tacaattatt ggaaaaagga
      421 atgaaactat gagaatgtcg cgctatcttg tcccaacact caaagaagat ccagctgatg
      481 cggtcattac cagccataaa ctcatgatac gcgcagggct tatccgcaaa gaatcagcag
      541 ggatgtatat ctatttgcca ttgggattac gggtattaca aaaaattatt actataatcc
      601 gtgaagagat gaataaagct ggtgcacttg agtttttaat gcctgagctt accaatgctg
      661 atttatggaa ggaatccggt cgctggcata caatgggtcc tgagatgata cgtattaaag
      721 accgcaatgg gttagaatat gcactggcac caacgcatga agaagcattc accaatgcca
      781 tccgcatgat agtaacctcg tacagggatt tgcctgtcaa tgcatatcag atcaatacaa
      841 aattccgtga tgaaattcgc ccacgatttg gtgttatgcg cagtaaagag tttatcatga
      901 aagatgcata ttcatttgac attgacgaag cggggttgga gcgttcatac cagacaatgc
      961 gccaaacata tcgcaatatc tttaaacggt gtgggttgga gacaattcct gtagaggcgg
     1021 atactggctc aatggggggc agcgattcag aagagtttat ggttgcatct gaggttggtg
     1081 aggaggtatt gctgctatgc gatgcatgcg gctataagtc aaatcaggag cgggccacct
     1141 gtcagtatga actatcgccc cagaacgaaa atgtggaagc actgagcatt gtggatactc
     1201 ccaatgtgcg caccattgat gaactggtac agttttttgg gtgcagtgca cataaatttt
     1261 tgaagagcct gatttatctt gctgatggaa atcctgttat ggcagtgctg cctggtgatc
     1321 gtgatataaa tgaagtaaaa ttaaaaaatg tgctgggtgc agttgaagtg gctctggcac
     1381 ctgacaaggt ggtggaagag gtgacaggag caccagttgg ttttgcaggg cctatcctgc
     1441 gtgaagggaa aacaatacgt attatctttg atacacaggt aaaacatatt gtgaacggca
     1501 ttaccggagc caatgaaaaa gacaaacact acaaaggagt aaaccccggc agagattttg
     1561 ttatcaaaga agaagctgat attacacgtg tggtagcggg agataaatgt cctcactgtg
     1621 gttatttact taaagaaaag aaaggtattg aggtggggca tatttttaag ttgggatata
     1681 aatatactaa gtcaatgaat gtcactgtgc ttgatcaaaa tggtaaggca atcacaccca
     1741 ttatgggctg ttacgggatt ggtgttaacc gaaccatggc agcagtaatt gaacagcatc
     1801 atgacgagcg gggaattata tggccaatga gcgttgcgcc gtttcatgta cattgtgtga
     1861 gcctggcaaa gaatgaagct gaagaggcca tggccgatga ggtgtattct atattgctca
     1921 aagctggcat agaggtgctg tatgatgacc gtaaggccag cccgggattt aagtttggtg
     1981 atgctgattt gattggtatt cccatccgca ttacgctggg caaggcattt tttggcgata
     2041 gttcctctat agaagtcaaa ctgcgaaacc aggatgaaaa ggtaatcatt gctaaagatt
     2101 ctatcgtcac atattgtctg aatctgattg ccgagagctt gaaaacgtta gaatcataga
     2161 tgtatgtgtg tgatgtgata tacttatctt catgggagaa gctatcacag taagcatact
     2221 ggtaatactg gcattagtgt atagtatcca gtatgttgta gcaaaattta caaaaccaca
     2281 gcaatgtgat tgtacacagt gccccatagc tgattgcaaa gaaagaaaaa ggactgtcta
     2341 aatgattgtt ttgatctgag tttctttgca gttacacagt tgattttatc atacaatgtg
     2401 gcaaaactgt attaccatga gtttgtcgtc cataattatg gttacacaat tcaagtaaca
     2461 accgtttttt agacagccct gaaaaatttt taataaaaga gtctgctata gttacgattc
     2521 tatagctact tcctcattgt gtttgctatt taatggttca ataaatataa cctggtttga
     2581 gacaagtttg atctttgatc ggggtttgcc agtatcatcc tgccatctgt cctgctttaa
     2641 tcgacctgtt gcctgaatct ttttcccttt tcgtatgttt tctgcacaca aatcggctaa
     2701 cttttcccat gtttcaatat caataaaaga aacatggggt tttgtttcac ccttaaccgg
     2761 atgatccata gctatggtaa aaatgcacac agatttaccg gtttttgttt ttcgtaaaat
     2821 gggatcatga gtaactcttc cttcaagatc gattggtttt gacatagtat cacctctttt
     2881 tagtagaatt tttggtaaga cgtttcatta tatacaacgc atgaagttgg aaaaattaac
     2941 gaatgaatga aaaatttttt taaaaaaatg aaaaattttt actatttttc tttataagct
     3001 ctaacgcagc atagaaactg tggaatgaat attctttgca caaacttttt gatttataaa
     3061 aaatataatt ctgttgacct tttgtatgct ttacccatgt tgtattgtag atgtttaaat
     3121 tttttgggag gatatatatg gctgaggaaa agaaatcacc caatcaggaa ttacaggaaa
     3181 agataagtgt aaaaaaagaa agcgcctggg cacaattgaa ggataaggtt gatagcatat
     3241 acagttttgg ggaagaatat aaattattta ttggcaagaa taagacggag cggctgtgta
     3301 ttcgcaccat ggttgagatg cttgagaagg ctcaattttc ttctattaat acaaaaggta
     3361 ccttaaaccc tggtgatgca gtatataaaa ttataaaagg caaaagttta ctggcctgta
     3421 aagtaggctc tgaccccatg aaggtgcgca tcattggcgc tcatgtggat tcaccacggc
     3481 ttgatttaaa gccgtatcct ctgtatgaag atgctgagct tgcactttta aaatcccatt
     3541 actatggtgg cataaaaaaa tatcagtggg tcaatatacc gcttgctata catggtgtgg
     3601 cattcacaaa agaaggaaaa acagtggaaa tacatatagg cgaaaatccc gatgagccgc
     3661 gatttattat tcctgattta ttgccacatc ttgccaaaga tcagatgaaa aaaacaggtg
     3721 aaacaatcat agaaggtgaa gatcttaatg ttatctttgc ccatatacct gttgatgata
     3781 aggatattaa agaaaagata aaattcaatg tgctgaaata tctccattcc acctatagcc
     3841 ttattgaaga ggattttgtt gctgcggata ttgaactggt acctgcgttg caacctgtgg
     3901 atataggtat tgaccgcggg cttattgcag cgtatggtca ggatgacaga gcttgtgcct
     3961 ataccgcatt tagggccttg cttgacgcac aggattgtaa agaaacagtc ctgctatatt
     4021 tagctgaccg tgaagaaata ggatccatgg gcgatacggg tgctgaaagc tttgtgtttg
     4081 ttaactttat gcgggagtat atacggaaaa ccggtataac cgcaacatgg gatgagcttt
     4141 tagaaaatgc cctttcaatt tctgctgacg taacaggtgc gctggatccc acattcaaaa
     4201 gcgtcaatga ccccaacaat gtatcgtatt taggacatgg tgttagcatt gaaaaatatg
     4261 gtggccgggg cggcaaatat tctaccagtg aagctcatgc tgaatacatg cattatttgc
     4321 gcagtttggc cactaaacac ggtataccct ggcaaacagg tgaacagggt aagatagata
     4381 ttggcggtgg tggaaccatt gccatgttta tgagccgtta cggcatggac tgtgttgatg
     4441 tgggtccctg catgttagga atgcattcgc cctttgaggt aacatccaag gttgatatct
     4501 actgtacata tttgttgtat aaggcttttt ttgaggatgc tcgataaagt aaaaagattt
     4561 attattgttt tagtaatgta ctaaggagtt ctccaatatt tgttgcatag atttttttat
     4621 ctatttgata tggtgttggt gtattacata caatatatga tgtggatact ctatcttcac
     4681 catagtaatc aataagttta aagatattat tacaatcttt tattgtagga ttttccgtta
     4741 gtttacattc aatagctatt atctttcctg atgcatgttc aataataaaa tctacttcct
     4801 tcccatgaat gtcttgccag taatagaggt ttaaacgttt accgtaaaaa gaataatgct
     4861 tcgtgatttc atttgcaata tagttttcaa agaaatatcc tgccatagaa gaattatgca
     4921 actgctgtgc atgttcaaag ccgcataaaa aagcagccaa tcctgtatca gtaaaataaa
     4981 ttttgggtga ttttattatt ctttttcctc tattagcaaa gtatggttca actaaatata
     5041 ctgcacctaa cgtagttaat acagataacc atttttttgc ggtattaacc gatatcccta
     5101 tgtctcgcgc taaatcagta tatgaaagta aattagaaat tcttaatgca caggaacgca
     5161 aaaatagatt aaattcgcgt aaatccgtta cattaagaac attgcgcacg tctctttcta
     5221 agtatgttgc cacatacgat gaataccata gattataatt ttttggggat gtatgtaatt
     5281 ctggatagcc tcctaaaaag ataaattcgt tctgagatat actggtatga ttggaaacct
     5341 cataaaatga aagcggataa agttgtaaca ttgcacatct gcctgataaa ctttctgaaa
     5401 caccctgcat gaatgaaaaa ttttgtgaac ctgttaggat aaattgacta ttcctcctct
     5461 gttgatctat ggcaactttt agtccctgga aaagctgggg tacatactgt acttcatcaa
     5521 taataacggg cctggcaaac ctttgaataa actcaagcga gtttgaggct gctgcaagct
     5581 ggtttgctgg cagatcaagg gtaatgtaag aagcatcagg aaacattttt cttagaaggg
     5641 atgtttttcc tacctgtcgt aatcctgtta taagcagtgc gggaaattgt tcaaaagcct
     5701 ggtaaacaag ggattctatt tttctttcaa tccacatgcg gatattttgc actaacaatg
     5761 caaaaagtca agcctaatta tggatttaaa cggtgatgca ggatttccat tatttccttg
     5821 ataagcccta aatattcatt ctgtgcgcta acaaactctt ttaccagtgg cttttctatt
     5881 aatgcttcac gaagctcaac tgacacagga tcgttttcaa tgtctatttc acctgattcc
     5941 attttctgat ttaacttttt accatgttca accaggcgcg atagtaactg taaagcttct
     6001 tcatcctcat tcatctgttg taaaagcgaa aaatatcgtg ttgccaccgg gtgctctgca
     6061 atgctattgg ctaactcata tgctttttgc tttatatctt caatgtcgct cattatttat
     6121 ttcctgcttt tttgttgata gtatcaaaaa aatctaatat gggtactact tcactgttcc
     6181 agtagataaa atcgtgcccg catgcacaat cattaaatgt aatagtaata tctggttttg
     6241 cagttttcaa tgcctcataa aaaatttttg tctgctcata gggaacaaca gtatcccttg
     6301 tgccatgacc aagataaaga ggaatgtacc attcagctat catagcaaaa gggttatcaa
     6361 cattttccca tcgattttta tagtattcat agggtccata cactgctgtc atcaatttgt
     6421 catgcttcat gcgtgtttga tcaaaatcac cggaaaggga agcagcagct ttgaaaatgc
     6481 ctttattgta caaagcggta agcactgcac ctcttccacc agtggatagc cccataacag
     6541 cattaaactg gttggataaa agtaagcctc gtttctgtag ctccgggata catatttctt
     6601 ttatccattg agatcccggt gtttttgccc atttgcgctt tgtttcagga tagtagtgtg
     6661 attcgtagat agtcaccgac atctcaggca ggataaggtt gtatcctcgc tttgtagctt
     6721 cctgtcgcag tgatgtttct tctatccagc gtttgcggct gaaattccat cctggcaaca
     6781 ccaggatgtt ttttttacag ttactatcgc ttatgataat atcaatggga attgttttac
     6841 ccgggtttat aactgcatca ggcaatgcaa tggtatgtag ccccggcgca agcgtacact
     6901 gtgcctgtga ctgatgggaa cagcacatca tgccagctgt aagcagtact atggataaaa
     6961 gaaaaaaacg tttcattgaa cgcgctctgc cagcatcaga ttggaattgc caatcccact
     7021 attatatcca tatgaaaaag gcagtggtcc tttagacagg gtgtcaaact tttctttcaa
     7081 ttctggctga tagcgatgag caaatagtgg taccggtttg gtgtagactc caaaaaatgt
     7141 aagtttccac tgatagggac tgaaaaactt aagtgccaca ccggaatcat cctgcaaaat
     7201 aaggtcactg tgttgcaggg taaggtcgcg aatcttggta aatttattgt cattatgcat
     7261 gagatatgac gctgatttta taatggttgt atagcgattg taggattcta aatgcggaat
     7321 aaaattggtg tagtacgata gtgaattgtc aataacattg atctggaaat accgcactcg
     7381 tttgatgtaa ccggcgttgt ccttgaaaaa aatctctgct ccaggtatgt atttgttttg
     7441 cttcataaca gatggcgttg ttgttatgag gctattgtca tcaatccaga ttttgtgcgc
     7501 attaatcact tcataatcaa gctgtcccaa aaaccacatg atgatgctta tcatactgtt
     7561 gaactcattg gtgctaactt ctttctgcat atcaatcgtt ttaaaaaagt tcatgttaag
     7621 gatttcatta agtgatgcac gcaatcccca taatcctgaa aatacacgtt ctttgcttac
     7681 cgcgtgggga tcaggaatat tgccgcatgg ttcaagtcca aacatgatat attcatcgcc
     7741 gtcagggaaa aaagcgatgg cgtttagtat atcagggcca ctgaatggat aaaagatagt
     7801 tttattgtag cgtgcaggaa ggttctgctt tttccattgt gctatttcat ctaagttttt
     7861 ctgctgcagc cgtgcccagc ttttccttaa ttcatccaca taggcaaggt aacgtttatc
     7921 agtactaagc ggaaaaagca aagatgaagg tgataacggt ttacctgcta aaaaccgtgt
     7981 ggcttccacg agtttggtat caatgggtgt tgcagcaaac ggtgactgtg tgcgtgctga
     8041 tgaattgtta tacaaaccac atgatatgat gcaacttatt acaaataata gagaaacact
     8101 ttttattttt gccatagata tcccttctgg attaatataa tattttagtt gcatctgtat
     8161 gtatgcaggt tattatttat gtcaatgatt ttatttaaaa tacagtacat actatagcgt
     8221 ctttgatatc aggcgtatac acgttcatct gatgcacaat gaacaatcaa gtttttgata
     8281 attactttac cgataacaaa ggaaaagttg ctccaccttc cttaaacaca gctactgttg
     8341 catttttgcc taagtgttta agtcctcgtt caatcacttc ctgaggttcg ttaaatcgtt
     8401 caaaaaatcc cagtgcctta acctcagtat ctgatagtga aggtacataa aaatagagat
     8461 tgtgtgcacg tataagctgc attgaataat aggttaaaaa cttctgctca acatcaaggc
     8521 caggtttaac tttttcaagg aatccaagta ctcgtgcggg gcctaatgtt cgcaaaattg
     8581 tacgtgttaa ccacagtggt gtggatttgt cagggagtgg aatatcatca agccctctct
     8641 gtgcacgcaa aaatcccatt acgatgcctt tgggccgcaa ggcaggcagg gtattcccta
     8701 cacatttcat gctctgctta aaattaatgt ccattggtgc tgaattggtt ataattccat
     8761 ccacctgttt atgtaatggc aatccgcata ttgtttttgt gaaagcaact gcttccctgt
     8821 gacagtgaat ggggtgacct gcaaagcagg caatgatgtc cttattacta tcgagaacaa
     8881 cattaataca aaaaacttca gctttaatca tattgagtgt ttcttccaga tcaagtctga
     8941 atgagttgtg ttctgcatca acggcaactc tgttaaaacg gtatggtggt tcagcaatga
     9001 ttgcatgatg attcccaatg gtttcagcgt acgcaacacc aggcagtatg ttcttaagcc
     9061 caccaccaaa tcctgcccac aggtgaggct caacaagacc tataagaatg ataaaatcag
     9121 catcttttag atgatggtta agatatactt caacaccgcg tgaggtttta ccaaaataag
     9181 catttttgtt cttgtcaaac gcatcgtggt tttcccattg cacctgtcca atattttttt
     9241 tgccaatctt ttcttccatt tcctcttttt tcattggagt gtgaataccc agtgcaggaa
     9301 taacaatagc attatgtttt gtggtacatc ctgccttttt aagtgcatcc agaacaatgt
     9361 caaaaaattt ataggctgga gtggggcggg tattgtcatc cacgacaatc acaacttttt
     9421 tatttttcaa aacgtgtttt tgaataggtt ttgctgaaaa agggttggcc aatgctcttg
     9481 caactatgtc tttttcattt tgttttttgg gcgatagttt atgctcctgt tgcctgggaa
     9541 taataacatc ccagctagca ggaagatcca gggatatgta tgaagcaccc catggtaaat
     9601 ctattttcat ggcaacctcc agtgaatgtg ttttttattt ttttacaata ctgttttgtt
     9661 tatgtcaata ttatttattg ttataaggct ctgaccccat agttaacaga atgtacagga
     9721 taaacaggat cttaaggatt tatctttatt atagctaatc ctttttatcc aatgtatcct
     9781 gtaatattta ttatgctctg acaccttttt ttaacaggat atacaggata tttaggatgt
     9841 taaggaaatt taatagtttt tcatcgcaat aataatcctt gatatcctcc ctatcctggt
     9901 attatcctgt tgctatgctc tgaccccatt ttttttactt ttttttctaa aaaataaaaa
     9961 attttcccac aatcccgtta atttttttca ccacctgcgt tctatagaat gagagaaaca
    10021 cacgtctctc atagtacaac aatatatgga ggaattctta tggctcggaa attacgggta
    10081 cagctaccag ggttaaccta ccacatcacg tcccgctgca tcgaatggaa atctatgttg
    10141 caggaagact actgtaaagc gtgctttgta gaaatactca acaaagcgaa agctaaatat
    10201 cactttaaac tcattgcctt ctgtattatg gataaccata ttcatttagt cattcatacc
    10261 ctggatgatg gtgctccaat atcccgtatt gtgcagtata ttaaggcacg gtttgctgaa
    10321 gtgtataata gaa
//
LOCUS       DAORYL010000096        10150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_4969_length_10150_cov_13.879346, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000096 DAORYL010000000
VERSION     DAORYL010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10150
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4969_length_10150_cov_13.879346"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1452
                     /locus_tag="PLV81_13415"
     CDS             157..1452
                     /locus_tag="PLV81_13415"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOM11137.1"
                     /translation="MSVLQSAMAAKEASIYLAAVSTSVKNKALQIIADEIKKNVEAII
                     QANTNDIQRSQEEHLPAPLLKRLKFDQAKINEVIAGIESLIALPDPVGNTLLATELDN
                     ALTLYKVSCPIGVIGVIFESRPDALVQISTLCLKSGNAVLLKGGSEAKETNKILAEII
                     YNAGVRAGIPEHWLTLLETREDVTALLKMDDYIDLIIPRGSNAFVKYIMDNSRIPVLG
                     HADGICHCYVDKDADLNMAVTVVTDSKVQYVAVCNATETLLVHRAIAHTFLPLIEQEL
                     KKYNVEIVGCEETQKIISVNPATEEDWKTEYLDYKLSIKVVDSVEDAIRHINKYGSKH
                     TDSIITSNKDTAKKFLDLVDSGNVFWNCSTRFSDGFRYGFGAEVGISTSKIHARGPVG
                     LEGLVIYKYKLIGNGNIVEDYAKGKRTFTHKKLNTQCPL"
     gene            complement(1455..3002)
                     /locus_tag="PLV81_13420"
     CDS             complement(1455..3002)
                     /locus_tag="PLV81_13420"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOM11138.1"
                     /translation="MTGFSQWFFSDYEHSDYLTKRKAFLVFIFALAVIVLLNSGVILS
                     FIFISVERGVGFLQSAIPASIFSLITMYYIKKGRLQLASNILVIFCSLVVLIGLFTKA
                     AHVGFVTMVYFMFATMLFASAFSTRVIASIIYGTYIVGLFVFYFLHADQVEGKLAEAL
                     KIGLIDSAAALTLSYLIVMMTITSFNRVIESLDIEKNKTIQQIGVLKSLHTIIEDISN
                     KMMSISSLISDKLKDFLQNIQDQASAVEEITASTQEVSNGFGNVNQNVNKQYNALKTL
                     FDTINALAQEIDLLKSRSVEISHAFESILTITRKGEDAIRIVNDNAKALLDSSGKLTS
                     IMNILQDIFDKIQLLALNASIEAARAGEAGRGFAVVADEVNKLSEQSVMSLKEINVNI
                     QSNIHSVETSNTGVATIVNLFQDIVSTLNTVSAGMQDIFKHIDNQEKIKEEIQTQVAG
                     SQTLSEQIAEDTNRHNEIIIEISNSIANINTLIQNNMSVAEDISDTSQELSNMGKDLL
                     NKIKEEA"
     gene            complement(3097..>4914)
                     /locus_tag="PLV81_13425"
     CDS             complement(3097..>4914)
                     /locus_tag="PLV81_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HOM11139.1"
                     /translation="FDDVDKQEATRGLIAEAKSLTINDNKGKVVWDMSAYSFLKKEKP
                     QTAHPLLWEMAQLNATPGLYQVVQGIYQIRGFDIANMAIIEGKTGWIVVDTLTTKETA
                     MAAFDFFKKNVKKKPIVAIIFTHSHIDHFGGVLGIITENDVKNKKITVIAPKGFMEEA
                     FSENILLANAMARRAEYQFGQDLPISPTGQIDSGIGKTAAQGSYSIVEPTVTVDTTPT
                     KMIIDGVEFIFQYTPDTEAPAEMTFYLPAYKAFCPAEIASRTMHNLYTLRGTKIRDAY
                     KWSKYINESLQLFPDVEVCFFTHNWPMYGNNRIREFLIKQADMYKFIHDQTIRLANKG
                     FTPEEIAHKLTLPQSLSLFMPNRQFYGVLQQNVKAVYQMYMGWYNGNPAYLNQLPDIE
                     RAKRYVEYMGGVKSILEKAGKSYNNGDYQWVAEVLNHVVMAQPDNQEARKLLAKTYTQ
                     LAYQAESGVWRNVYLKAAQELSTGMQKKKSVMFKGYDMLKHMPPEKLFDSMAVNLNPE
                     KAEHSSISIAIHFSDKNASYMLYIRNSVLYYSKGIIGKQEADLHIPYTTFIKLITGAI
                     DKTKILFDKDVQWNGSNVALIKFFSMFEKQERFAIVTPR"
     assembly_gap    4915..5014
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5018..5836)
                     /locus_tag="PLV81_13430"
     CDS             complement(5018..5836)
                     /locus_tag="PLV81_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOM11140.1"
                     /translation="MKSLKGQLALVTGAAMGMGRSLSELLLDEGCEVAMVDINEKELT
                     KAVKQLQTKGNCRAYICDVSKRDAVYKLQKKVKKEMGDVTILVNNAGVVRVGNFLQLE
                     DKAIEWIVNVNLMSIFWMCKAFMPDMIHKPWAHVVNFASAGGMLALPDISIYCATKFA
                     VVGFSDALRQEMKKYKYNVGVTMVCPYTVATGMFAGFKEVAGTKILKTEEVTTRVVKA
                     IKKNKPLVAVPNFQVNFLTPLTKLILPVHAMDFVNKLAGMWTANEQTHERKYKI"
     gene            complement(5975..>6277)
                     /locus_tag="PLV81_13435"
     CDS             complement(5975..>6277)
                     /locus_tag="PLV81_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11141.1"
                     /translation="YRKKGSSWKRFHIVLYEDEYEFFMDVKKIWKMSLAKIIEFCLDN
                     VLEEFLKILDNIGSDDYTDNYRHTGYTFCFYREEDIICCQFYWGPHPDLVRKSKIV"
     assembly_gap    6278..6377
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6409..7311)
                     /locus_tag="PLV81_13440"
     CDS             complement(6409..7311)
                     /locus_tag="PLV81_13440"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOM11142.1"
                     /translation="MARKIIIACITIIVFLCVIVFGAAWHFSNQLINPKPYTCQVDHF
                     VYCDGIGELSIPYKDVTFKNNENLTLKGWLVPGKSNAAIVMVHGITADRREGLRWVKA
                     LHKAGYTVLLFDLRNHGKSDKAKTAMGYYEKNDVIAAVDFLQSKGFKNIGVFGVSMGA
                     STAIQAMASDKRIQAGVFEAAFANLSDLLAEIAKRDFGLPRFPIINAVMWVYSMRLGA
                     DANGINPEDFIGSISPRPVFIIHCDSDDYIAYHHGQRIFAKAKEPKIMWTAHCNKHAR
                     AWQSNPQEAEKKVVDFYNKFISSK"
     gene            8037..9524
                     /locus_tag="PLV81_13445"
     CDS             8037..9524
                     /locus_tag="PLV81_13445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOM11143.1"
                     /translation="MWEFAPTSVHWLDIVLIIGYLAGITIFGFYYKSHVKTAKDYFLA
                     NRSLPWWVIGLSIIGTNIGSNDYIGAAGNAYSIGIAQANFEWIGAIPAMILSALVFIP
                     FFWRAGVYSIPEYLGLRYNNAVRFIAACVMSVFAVVIVGVFLWATAIMLQTYLGWPIW
                     FSILVTATVVGFYTISGGLGAVAITDTVQTFIMFFTAIIVAFIGIEKIGGFDMFIKTL
                     QNNYPDHIHSFLPANHPVFPWPGVILGLGFVLSPAYWCANQAILQRTFAAKSEWDGKA
                     SMMFAACLKTFVPFLIVMPGLLALTLAGKSITHQDQALPWVIKHILPAGISGLMFVAF
                     IAALQSSIDSTMNSTSVMIVRDIINVAKKTKLTDRAQLHLGKLFTFIILIVSIAFAPI
                     TAYFQGIYVYVQYALSLFQGPIFALMLFGILDKRITPSAGLFSMISGLAVAAVLGWLQ
                     FNMLYIAFLSFVYSAIALYIVSLLTKRKPEAELKNVVYSNVVEID"
     gene            9540..9743
                     /locus_tag="PLV81_13450"
     CDS             9540..9743
                     /locus_tag="PLV81_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11144.1"
                     /translation="MTEILYSLPLWYAKVTAMLIFLGMLVVAWLLPYNFVVQGAPDKK
                     RWRDLRLWATLLTIVQLFIYYIF"
BASE COUNT         2975 a         1954 c         1735 g         3286 t
ORIGIN      
        1 tttttagagt taggtaacac attggaagag tgtattgaac gtctgtgtgc ttattgccgt
       61 ggtgagcttt tgctctgaca ccagtaaaaa tatattgaca caaataccaa aacttttacc
      121 caataacact ataattatac cagaagaggt catcccatga gcgtattaca atctgcaatg
      181 gctgcaaagg aagcatccat ttatctggca gctgtttcaa ccagcgttaa gaataaggca
      241 ttacagataa tagctgatga aatcaaaaaa aacgtggaag caattataca ggctaatacc
      301 aatgatatac agcgcagcca ggaagaacac ctgcccgcac cattgctcaa acgcttgaaa
      361 tttgatcaag ccaagattaa tgaagttatt gcaggaattg aaagcctcat tgcattacct
      421 gatcctgtgg ggaataccct tcttgcaaca gagcttgata atgctctgac cctctataaa
      481 gtaagctgtc ctattggtgt gattggtgta atttttgaat cacggcctga tgctctggtg
      541 cagatatcaa cactgtgttt gaaaagtggc aacgcggtac tccttaaagg tggcagcgaa
      601 gcaaaagaaa ccaacaaaat tcttgcagaa ataatttata atgcaggtgt cagagctggt
      661 ataccggaac actggctgac actgcttgaa acgcgtgaag atgtaacagc gcttttaaag
      721 atggacgatt acattgacct tattattccc cgcgggtcaa atgctttcgt caaatatatc
      781 atggataact cacgcatacc ggtgcttggc catgcggatg gcatttgcca ctgctacgtt
      841 gataaagatg cagacctgaa catggcagta actgttgtta ctgattccaa agtacagtat
      901 gttgcagtgt gtaatgctac tgaaacattg ctggtacaca gagctatcgc acatactttt
      961 ttgccgttaa ttgaacagga acttaaaaaa tataatgtgg agatagttgg atgtgaggaa
     1021 actcaaaaga ttatttccgt aaatcctgca accgaagaag actggaaaac agaatacctg
     1081 gattataaac tttctattaa ggttgttgat agtgttgaag acgcaatcag gcatatcaat
     1141 aaatatggtt caaaacatac cgacagcatt attaccagca acaaggacac agcaaagaaa
     1201 tttcttgatc tggttgattc aggcaatgtc ttctggaact gttcaacccg ttttagcgat
     1261 ggatttcgct atggctttgg cgcagaagta gggatcagca cttcaaaaat tcacgcacgt
     1321 ggaccggttg ggttagaagg tctggtaatt tataaatata aactaattgg aaatggcaac
     1381 attgtagaag actatgcaaa aggcaagcgt acgtttacgc ataaaaaact gaatacacag
     1441 tgcccgttgt aatactacgc ctcttcttta attttattga gcaaatcctt acccatgttc
     1501 gatagttcct gtgatgtgtc gctgatatct tcagcaacag acatattatt ctgaatgagt
     1561 gtattgatgt ttgctattga atttgaaatc tcaataatga tttcattgtg gcgattggta
     1621 tcttcagcaa tctgttctga aagagtctgt gagccagcaa cctgagtttg tatttcttct
     1681 tttatttttt cctgattgtc tatatgcttg aatatgtcct gcatgccagc actgactgta
     1741 tttaatgtgg atacgatatc ctgaaacaaa tttacaattg ttgcaacacc ggtattagaa
     1801 gtttccacac tgtgaatatt ggactgaata ttgacattaa tttcttttaa actcataact
     1861 gattgctcag aaagtttatt aacctcatcg gcaaccaccg caaagcccct tccggcttcg
     1921 ccagcacggg cagcctcaat tgaggcgtta agtgccaata gctgtatctt gtcaaatata
     1981 tcctgtaaaa tgttcataat tgaggttaat ttgccagagc tatctaacaa tgctttagca
     2041 ttgtcattta ctatccttat agcatcttcc ccttttctgg tgatagtcag tattgattca
     2101 aacgcatggg atatttcaac cgacctgctt tttaataaat caatttcctg cgccagggca
     2161 ttaatagtat caaagagtgt tttcaatgca ttatactgtt tgttgacatt ctggtttaca
     2221 ttgccaaaac cgttcgacac ctcctgcgtt gatgcagtga tctcctcaac agcagaagcc
     2281 tggtcctgaa tattttgcaa aaaatccttc aatttatcag aaataagtga agatattgac
     2341 atcattttgt tggaaatatc ttcaataatg gtatgcaagc ttttcagcac accaatctgt
     2401 tgaatggttt tatttttttc aatatccagc gattcaataa ccctgttaaa tgaggtaatg
     2461 gtcatcataa caatcaaata tgaaagcgtc aacgctgcag cgctatcaat cagtccaatc
     2521 ttcaatgctt cagccagttt cccttcaacc tggtcagcat gtaaaaagta aaacacgaat
     2581 aatcccacta tatatgtgcc ataaatgatt gaagcaataa ccctggttga aaatgcagaa
     2641 gcaaatagca tggtggcaaa cataaaatac accattgtaa caaatccaac gtgagcagct
     2701 tttgtaaaaa gacctataag taccaccaga gagcaaaaaa taaccagaat attggaggcc
     2761 agttgcaatc tccctttctt aatataatac atggtgatta aagaaaagat tgatgccggg
     2821 atagcagact gcaaaaaacc taccccacgt tcaacactga taaaaataaa tgatagtata
     2881 acacctgaat tgagcaatac aataactgcc agcgcaaata taaataccag gaatgctttt
     2941 cgtttcgtaa ggtaatcact gtgttcataa tcagaaaaaa accattggga aaatccagtc
     3001 atgataacac ctcaactata taacatttat ctgtttctat tgacagtaag ctaaactaca
     3061 tatatagtca ataactattt atcgttacca tactaattag cgtggtgtta ctattgcaaa
     3121 gcgttcctgt ttctcaaaca tagaaaaaaa ttttatcagt gctacgttac tgccattcca
     3181 ttgaacatcc ttatcaaaaa gtatttttgt tttgtcgata gctccggtaa taagcttaat
     3241 gaaagtagta tagggtatgt gcaggtcagc ctcttgcttg ccaattattc ctttagaata
     3301 atacaacact gaattgcgaa tatacagcat atatgacgca ttcttatcgc taaaatgaat
     3361 tgcaatgctt atgctgctat gttctgcctt ttcaggattt aagtttaccg ccatgctatc
     3421 aaataatttt tccggtggca tgtgtttcag catatcatat cccttaaaca tcaccgattt
     3481 tttcttttgc atgccagtgc ttaattcctg tgcagctttt aaatacacat tgcgccatac
     3541 accagattca gcctgataag ccagttgcgt gtatgttttg gcaaggagct tccgtgcttc
     3601 ctgattgtcg ggctgtgcca ttaccacatg atttaacacc tcagcaaccc actggtagtc
     3661 accgttgtta tatgatttcc ctgctttttc aagtatgctt ttgaccccac ccatatattc
     3721 tacataccgt ttagcccttt ctatatcggg cagttgattg agatatgcag gattaccgtt
     3781 ataccatccc atatacatct ggtacacagc cttaacgttt tgttgcagta ctccgtaaaa
     3841 ctgtcggtta ggcataaaga gcgatagtga ctgtggaagc gttagtttat gagctatctc
     3901 ttcaggtgta aatcctttgt ttgcaagccg gatggtctgg tcatgaataa atttatacat
     3961 gtcagcctgc tttataagga attcccttat gcgattgttg ccatacatgg gccagttatg
     4021 tgtaaaaaag cacacttcaa catcaggaaa aagctgtagt gactcgttaa tgtatttact
     4081 ccacttatat gcatcccgaa tttttgttcc gcgtaacgta tacagattat gcatggtgcg
     4141 acttgcaatc tcagctgggc aaaaggcttt atacgcaggt aggtaaaacg tcatctctgc
     4201 tggagcctcg gtatcgggtg tatactggaa tataaattcc acaccatcga ttatcatttt
     4261 cgtaggagtt gtatcaacag taactgtcgg ctccacaata ctgtaggaac cctgtgccgc
     4321 agtttttcca ataccactat ctatctgccc tgtgggcgaa atgggcaaat cctgtccaaa
     4381 ctgatactca gctcttcgtg ccattgcatt ggcaaggagt atattttcac tgaatgcttc
     4441 ttccataaac cctttaggtg ctatcaccgt tattttttta ttttttacgt cattttcagt
     4501 aattatcccc aatacaccac caaaatggtc aatatggcta tgggtaaaaa tgattgctac
     4561 aatgggcttt ttcttcacat tctttttaaa aaaatcaaat gcagccattg cagtttcttt
     4621 tgtggtaagt gtatccacaa caatccagcc tgttttccct tcaattatgg ccatatttgc
     4681 aatgtcaaag ccccttattt gatatatacc ttgtacaacc tggtataatc ctggtgttgc
     4741 attcaattgt gccatttccc atagtaatgg atgtgccgtt tgtggttttt ctttttttaa
     4801 aaacgaatat gctgacatat cccacacaac cttgcctttg ttatcgttta tggtcaggct
     4861 ttttgcttca gcaattaaac cacgtgttgc ttcctgttta tcaacatcat caaannnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatttta aattttgtat ttccgttcat
     5041 gggtttgctc atttgctgtc cacataccag ctaatttatt aacaaaatcc attgcatgga
     5101 cgggaagtat aagcttggta agtggtgtta agaaattaac ctgaaaattg gggaccgcta
     5161 ccaatggttt atttttctta attgctttta ctacccgtgt tgttacttct tcagttttaa
     5221 gaatttttgt acctgcaact tccttaaaac ctgcaaacat cccagttgcc acagtgtatg
     5281 gacataccat ggttacacct acattgtatt tgtatttttt catctcctga cgcaatgcgt
     5341 ctgaaaatcc tacaactgca aatttcgttg cgcaataaat tgatatatcg ggcaatgcta
     5401 acattccacc agccgaagca aaattaacaa catgtgccca tggtttatgt atcatatcag
     5461 gcataaatgc cttacacatc cagaagattg acatcaaatt tacattgaca atccattcaa
     5521 ttgctttatc ttccagctgt aaaaagttgc ctaccctgac aacgccagca ttgttaacaa
     5581 ggatcgtgac gtcacccatt tctttcttta cttttttttg tagtttatat accgcatcac
     5641 gttttgaaac atcacatata tatgccctgc aattgccttt tgtttgtaac tgcttgacag
     5701 cttttgttaa ttctttttca ttgatatcca ccatagcaac ttcacaacct tcatccagca
     5761 gcaattcgga taaacttctg cccataccca ttgcagcacc agtaaccagc gctaattgac
     5821 cttttaatga tttcatataa tgcctcctgt ttattattgg taaatattaa tttgcttaat
     5881 taaaaaatca actatttttt ggatgattgt ccaatttatt acagacactt aataaaaaag
     5941 attattaaat gtgcagataa atgtacatta attcttatac tattttggat tttcgcacta
     6001 aatctgggtg tggtccccaa taaaactgac agcaaataat atcttcttct ctataaaagc
     6061 aaaatgtata tccagtgtgc cgatagttat cggtataatc atcacttcct atattatcta
     6121 agatttttaa aaattcttcc aggacattat ctaaacaaaa ttcaataatc ttagccagag
     6181 acatcttcca gatcttcttc acgtccataa aaaattcata ttcatcttca tataacacaa
     6241 tatggaaacg tttccatgaa ctaccctttt tacggtannn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnaaa tatttatctt atatttttaa ccaaaatttt atttggaact
     6421 aataaattta ttataaaaat caaccacttt tttttcggct tcctgtggat tggactgcca
     6481 tgctcgtgcg tgcttattgc aatgcgctgt ccacataatc tttggttctt tggcttttgc
     6541 aaaaatgcgc tggccatgat gataggctat ataatcatca ctatcacagt gtataataaa
     6601 tacgggacgt ggtgaaatac tcccaataaa atcttcagga tttataccat tagcatctgc
     6661 tcccaaccgc atgctataca cccacataac tgcattaatg ataggaaatc gtggcaggcc
     6721 aaaatcacgt tttgctattt cagcaagcaa atcgctcaaa ttggcaaaag ctgcttcaaa
     6781 tacaccagcc tgtatgcgtt tgtcactagc catagcctga attgcagtgg aagctcccat
     6841 tgatacacca aatacaccaa tgtttttaaa gccctttgat tgtaaaaaat caactgctgc
     6901 aatcacatca tttttttcat aatagcccat ggctgttttt gctttatcgc tttttccgtg
     6961 atttcgaaga tcaaataaca atacagtata tcctgcttta tgcagtgctt ttacccaccg
     7021 caacccctca cgcctgtcag cggtaatgcc atgaaccatt acaattgctg catttgattt
     7081 tccaggaaca agccaccctt tcagggtaag attttcatta ttcttaaatg taacatcttt
     7141 atatggtata ctcagttcac ctataccatc gcaatataca aaatggtcaa cctgacatgt
     7201 atatggtttt gggttaataa gctgatttga aaaatgccag gcagcaccaa agactatgac
     7261 acaaagaaat acaataattg ttatacatgc tataattatt tttctagcca tgtcattcct
     7321 ccttgagtag caatgaactt gaaatgatat ggtgtttcac cattaatcca ctaaaatacc
     7381 ggaaaatctt gattttaaaa aaaacaactc catctgcctt acatacagat taatgttgca
     7441 gaatggcttg cacatgagcc atacttctat atctatgata cccaatagca ttgattactt
     7501 aaaaaaaagc agaacctgaa gtacatttat ataatcgtta acgacatttt gcaagcataa
     7561 tatttttgat tttactcatt taaccaaatt tttcttaatt actgcataat caccttttag
     7621 acaaccccgg agcctttttt ataaattaaa accatcggta aatcaccaaa aacaattatt
     7681 ttagcaaagg taaggccttc tacttcctca taaatcatta aaatcctgta aaacttcagc
     7741 cagattttct ttggcagttt cttttacaat cttttctgtt aattgcaatg gttcatatac
     7801 taactactat catcagttaa gaataatttg aggccaatat tataatgtta ttaatgcact
     7861 tttaaatatg tacgataata agtgcaaaaa attatttcag gtaaacataa tactatatag
     7921 caaaaattta tgtttcagta actatcgctg cataaaaaat ggttgattct tgatacattt
     7981 atttattata atcgataacg acaattttta tacacttcag tatatgagga aacgccatgt
     8041 gggaatttgc gccaaccagc gtgcattggt tagatattgt attgattata ggctatttag
     8101 caggaataac catctttggg ttttattaca aatctcatgt taaaacagca aaggattatt
     8161 tcttagccaa ccggtcattg ccatggtggg ttataggcct ttcaattatt ggcaccaaca
     8221 ttggttctaa cgattacatt ggtgctgctg gcaatgctta cagcattggt attgctcagg
     8281 caaattttga atggattggt gcaatccctg caatgatact atcagcactt gtctttattc
     8341 catttttctg gcgtgcaggc gtttattcaa tacctgaata tttaggattg cgatataata
     8401 atgctgtgcg ttttattgct gcctgtgtca tgagtgtatt tgcagtggtt attgttggag
     8461 tgtttctgtg ggcaaccgcc attatgctgc aaacatattt gggctggcca atatggtttt
     8521 caatactggt aacagcaacc gttgttggct tttatactat atcgggcggg cttggcgcag
     8581 ttgctatcac cgataccgtt cagacattca tcatgttttt tacagccatt attgttgctt
     8641 ttattggtat tgaaaaaatt ggtggtttcg atatgtttat aaaaacatta caaaacaatt
     8701 accctgacca catccattca tttttgcctg ccaatcatcc agtattccca tggccaggcg
     8761 taatactggg acttggcttt gtgctttctc ccgcatactg gtgtgccaat caggcaattt
     8821 tacagcgaac ctttgcagcc aaaagtgaat gggatggcaa agcttcaatg atgtttgctg
     8881 catgcctcaa aacgtttgta ccatttttaa ttgtaatgcc agggctactt gctctgaccc
     8941 ttgcaggcaa aagtattacc caccaggatc aggcattgcc atgggttatt aaacatattc
     9001 tacctgcagg catttcaggc cttatgtttg ttgcattcat tgctgcttta cagtcatcga
     9061 tagattcaac catgaattcc acctcagtta tgattgttcg ggatattatt aatgttgcca
     9121 aaaaaactaa actgactgac agagcacaat tgcatctggg gaaattgttt acatttatca
     9181 ttcttattgt aagcattgca tttgcaccaa taacagctta ctttcagggt atttatgtgt
     9241 atgtgcaata tgcgttatcc ctttttcagg ggccaatttt tgccctaatg ctctttggta
     9301 tcttagataa acgtattaca ccttcagctg gtctttttag catgatatcg gggctggctg
     9361 ttgcagcagt cttaggctgg ttacagttca acatgctgta tatagcattt ctttcatttg
     9421 tgtattcggc gatagctctg tatattgttt ccctgcttac gaaaagaaaa ccagaagctg
     9481 aactgaaaaa tgttgtgtat agcaatgttg ttgaaattga ttaattggtg gagacccgta
     9541 tgacagaaat actgtattca cttccgcttt ggtatgcaaa agttacagca atgcttattt
     9601 tcttaggcat gcttgttgtt gcttggcttt tgccgtataa ttttgttgtc cagggtgctc
     9661 ctgataaaaa acgatggcgc gatttacgac tgtgggcaac cttattaact atagtacagt
     9721 tatttattta ttatattttt tgaatagtat atagcaaatc ataaactcac tacgatacat
     9781 attaaaaaca aaatattttt ttaccctggt tgcttctatt attttattat taagttcata
     9841 tgtttcagcg cgggaagaat atgcatattt tatgccatca gggaaaatgt ttttgacttt
     9901 caacggggat ttatccctga aagggaatgg ctacgggagt aaagcatcaa tagttgatgc
     9961 aaacagtgaa gatacgttgg atggtgaatt ggacttgttt taggaataac tgctgtgtat
    10021 tgaaaaacaa agatattttt tagttttagt tttacaatga aaggtgagta tacgtctact
    10081 cacctttatt tattttttta ttattttctt gccattttag tttagttatt ttttataaat
    10141 atatcattta
//
LOCUS       DAORYL010000097        10018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_5062_length_10018_cov_15.355515, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000097 DAORYL010000000
VERSION     DAORYL010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10018
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5062_length_10018_cov_15.355515"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PLV81_13455"
     CDS             <1..396
                     /locus_tag="PLV81_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOM11145.1"
                     /translation="CYNMTKAATISLSETLSVELSPYNINVSVVCPTFFKTNLMDQFT
                     SPDKRQLIIAQKFFDRSHVSAEEIARHTIRNMSKKHFYIIKQPDGKLIWWFKRHVPEW
                     YFKLLSFIYRKGYFYNYLKITPQEMSERR"
     gene            400..1143
                     /locus_tag="PLV81_13460"
     CDS             400..1143
                     /locus_tag="PLV81_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOM11146.1"
                     /translation="MKELLENTGPGTYTEGMPWNPVEQVIFNRRSIRSFKEEPIPDAM
                     IRRILEAGRFAPSAGNFQPWRFIVIKDKKILTEMEKDAVRFIKLLMFFLDYTKGGFLR
                     RLLVKPIAKIVIRFKPNEFHPVPFGLMSQIAKGQTLVFHNAPTLILMLKDRRGVSNPD
                     IDIGICGQNMVLAAHSLGAGTCWIGLIKVLMYFPKWRKLFNITYPYQLDVCLALGWQK
                     PKADGPVPREVQLVEWFDNGTIRIEKQGE"
     gene            1147..1521
                     /locus_tag="PLV81_13465"
     CDS             1147..1521
                     /locus_tag="PLV81_13465"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOM11147.1"
                     /translation="MEIPFSFIDRIIIPDYDTPKGVQGGNLEFNELCKECGTCIRICP
                     GGCLQSTTTTKMDIINKLQKGKKGIPYVISTQRGTTLCVSCFDCGAACPQQAISIRCN
                     FNPQYFFKRLTQLANMQPPKRY"
     gene            complement(1598..2650)
                     /locus_tag="PLV81_13470"
     CDS             complement(1598..2650)
                     /locus_tag="PLV81_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11148.1"
                     /translation="MKKIIIIVLVFVCTAAYARTTVNLTPADANFSDIPVLEDSLGKQ
                     VQDVTDKNGKFIANSFALANMLGYPIGKSSIGHFPHMEAGIALGVALTNAKYFDDKAE
                     DGTFPGVMANPVVHAGFGLAGNFDIIGKLFYFRKNIYDPDIDTDTARLQDFNFISFGA
                     KLRYNYLKEATILPFLLSFGGLTFSIGGDIMMGNVDVTGEYDAEYEDITVTYSSIDYP
                     LSANLNSTYGATVSWTVFTLSAQAIAYIDVMYLFSFYTGFGLATNLGFFSTDFNGNGQ
                     LTSDDPTFLGVKADGLIGTMEFESVNSYMPGYLIPTFIFGVEINLFVIKLTADTMVNL
                     ANRSDVTLQGGVRMQM"
     gene            3185..3934
                     /locus_tag="PLV81_13475"
     CDS             3185..3934
                     /locus_tag="PLV81_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOM11149.1"
                     /translation="MTLKNKILIILSLCLLLCVGAYFIISSTFANFEKQLFEKCRIEA
                     LVGARVMSELIEMMIDTRILTKEQVFDRNYIPIPNTNPQKFRTKYDAIFDRYIQKIED
                     EFLKDEDLEFAALVDINGYLPTHNSKYAKPEMTDPVYNLKYSRSKRIFNDMVGLNASR
                     FIGPGTLKQLYNRDTGEIMWDISAPVFVKGEHFGAFRIGVSLKRINELKNQMIIIVGM
                     TILVILSITMLMLFLILPRKLYDTDLDIPQY"
     gene            complement(3931..>4653)
                     /gene="folE2"
                     /locus_tag="PLV81_13480"
     CDS             complement(3931..>4653)
                     /gene="folE2"
                     /locus_tag="PLV81_13480"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF010200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HOM11150.1"
                     /translation="ITYPVVVLDRADGLQHTVARINMYVDLPEHFRGTHMSRFVEVLN
                     RYQGGISTKNVRDILEDLKKELNAKTSHVNIEFPYFITKTAPVSGEKSKMDYICTLQG
                     DSSVDYYTLQVKVPLLSVCPCSKEISQYGAHNQRSVVSVSVRAREILWIEELIELVEE
                     CGSSQIYALLKREDEKYITERSYENPVFVEDIVRNVAVKLLAHPKVLWFLVETESMES
                     IHNHSAYAQIEIDKSKMKRSLA"
     assembly_gap    4654..4753
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4754..5583)
                     /gene="recA"
                     /locus_tag="PLV81_13485"
     CDS             complement(<4754..5583)
                     /gene="recA"
                     /locus_tag="PLV81_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOM11151.1"
                     /translation="MSEQKELNQKSQALESAILQIEKQFGKGSIMRLGDEHAKMKVPA
                     ISTGSLELDIALGIGGIPRGRITEIYGPESSGKTTLTLHVIAQAQKAGGIAAFVDAEH
                     ALDPVYAKKLGVNTDELLVSQPDTGEEALEITESLVRSGAIDIVVIDSVAALVPRAEI
                     EGEMGDAHMGLQARLMSQALRKLTGVISKSKTSVIFINQIRHKIGVMFGSPETTTGGN
                     ALKFYASVRLDIRKVDTLKAGEDAVGNRVRVKVVKNKVAPPFRQAEFDIMYDSGISRT
                     A"
     gene            complement(5910..>6128)
                     /locus_tag="PLV81_13490"
     CDS             complement(5910..>6128)
                     /locus_tag="PLV81_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11152.1"
                     /translation="KNIFKEECRVMSRIIMMVCSVVVATFIIVGLIDYGYSGCVVGQK
                     SCEDDGYYWVCETCGSETCWIFTGERCK"
     assembly_gap    6129..6228
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6229..7772)
                     /locus_tag="PLV81_13495"
     CDS             complement(<6229..7772)
                     /locus_tag="PLV81_13495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOM11153.1"
                     /translation="MPVTKEEALKLSWNNVDIVLVTGDAYVDHPSFGIAVIGRVLEKA
                     GFSVGIIAMPNFLDPSSVEVFGRPNLFFGVTSGNIDSMLSRFTAFKKIRNDDPYIPGG
                     KGGIKPSNAVITYCNLIKQRFKDVPIVIGGIEASMRRLLHYDFVQDKLRRSIMVDSRA
                     DILVYGMGEYQILEIARRLSGGQSLQGIPGTVTIAKQKPDDAVELPTEEDALANVESL
                     LVMYKMFYQNFGTKVIAQKSGGRYIIHTPPPAVTRKELDAYYELPYERKPHPMYKDTI
                     PAFEMIKYSINAHRGCAGGCSFCSLTLHQGKMVVSRSADSIKQEIVTVSKRKVHISDI
                     GGPSANMYGFTCAIGGCNRESCVFPAVCKHLRLATDKWLNLMEDALTLPNVKKVSVGS
                     GIRYDMFMQGSNAKKNLEFLVKHFISGQLKIAPEHTDKAVLKAMRKVSAIPLEEFVNA
                     FNAACKRLGREYYLVPYLMTCHPGSTDSSVYRMRKDIAALFGYVPYQCQAFIPLPMTL
                     SSIQYYT"
     gene            complement(7788..9374)
                     /locus_tag="PLV81_13500"
     CDS             complement(7788..9374)
                     /locus_tag="PLV81_13500"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOM11154.1"
                     /translation="MTVTKSKQNKVFIFDTTLRDGEQAPGFSMNIDEKLHFADQLVKL
                     GVDIIEAGFPISSPGDFEAVKRISERVKGVQVAGLARANFKDIDVAWDALKYADNPRI
                     HTFISSSDIHIQYQFKKTREEVLAIAVDAVKHAAKYTSNVEFSPMDATRSDWSYVAQM
                     VEAVIKAGATTVNIPDTVGYAIPSEFHEFIKFLFNTVPNIDKAIISVHCHNDLGLATA
                     NALAAIEAGARQVECTINGIGERAGNTAMEELVMALKTRGEKFQVYTDINSKQIMSTS
                     KLLTHITGVSVQPNKAIVGANAFAHESGIHQDGVLKNAMTYEIMKPEDVGITKSTLVL
                     GKHSGRHAVIARLNSLGYNLTDEELERFFKLFKVLADKKKQIFDEDLEVLVGEAVYKM
                     KGRYKVTNVQISTGMYSPPMTLVTIKDRYNDGVETFDVAHGNGGVDAGINAVKKITGT
                     QAKIESFNLVAITGGSDALCEVTVTVVEEINGRQLKVFGNGVNIDISVAGILSFVDAL
                     NKLEYIKQRNHIGEKIEAKL"
BASE COUNT         2941 a         2173 c         1789 g         2915 t
ORIGIN      
        1 tgctacaata tgacgaaagc agctacaata tctctaagcg aaacgttgag tgtggaacta
       61 tcaccttaca acattaatgt atccgttgta tgtccaacat ttttcaaaac aaaccttatg
      121 gatcaattta cttccccgga taaacgtcag cttataattg cacaaaaatt ttttgaccgt
      181 tcgcatgttt ccgctgaaga aatagcacgt catacaattc ggaacatgtc aaaaaagcac
      241 ttttatatca taaaacaacc tgatggtaaa ctcatctggt ggtttaaacg ccacgtacca
      301 gaatggtatt tcaagctttt atcgttcata tacaggaaag gttattttta taactatcta
      361 aaaataacac cccaggaaat gagtgaaagg aggtaaacca tgaaagaatt attagaaaat
      421 accggtcccg gtacctatac cgaaggaatg ccatggaatc ccgtagaaca ggtaattttc
      481 aaccgcagaa gtattagatc attcaaagaa gaacctatac ctgatgcaat gatacggcga
      541 attcttgaag ctgggcgatt tgctccttca gcagggaatt tccaaccatg gcgctttata
      601 gtgattaaag ataagaaaat actgactgaa atggaaaaag atgcagtgcg ctttatcaaa
      661 ctcttgatgt tttttcttga ttacacaaaa ggtggattcc ttaggcgttt acttgtaaaa
      721 ccaattgcca aaattgttat acgatttaaa cctaatgaat ttcatccagt cccttttggt
      781 ttaatgtctc aaattgcaaa ggggcaaaca ctagtttttc ataatgcgcc aacactaatt
      841 ttaatgctaa aagaccgtcg tggtgtatcc aatccagata tagatattgg aatatgtgga
      901 caaaatatgg tgctggcagc acatagttta ggtgcaggaa cctgctggat tggtctaata
      961 aaagtattaa tgtatttccc aaaatggcgt aaattgttca atattacata tccatatcaa
     1021 ttagatgttt gtctggcttt gggctggcaa aaaccaaaag ctgacggtcc tgtcccacgt
     1081 gaagtacaat tggtagaatg gtttgataat ggtactataa gaattgaaaa acagggggaa
     1141 taactaatgg aaattccatt ttcttttatt gataggataa taatcccgga ttatgatact
     1201 cccaaaggag tccaaggagg taatcttgaa tttaatgaac tttgtaaaga atgtggtacc
     1261 tgcatccgta tttgccccgg tggatgttta cagagtacca caacaactaa aatggatatt
     1321 attaataaac ttcaaaaagg gaaaaaaggg attccgtatg ttatctccac acaaagagga
     1381 actacgttat gtgtaagctg ttttgattgc ggagccgcct gtcctcaaca agcaatcagt
     1441 attagatgca attttaatcc gcaatatttt tttaaacggc tgacacaatt agcaaatatg
     1501 caaccaccaa agcgatatta aactgaccgt ataaactgga cagtactggt agctaattta
     1561 tatttttgca ttgcgaatat gcgctttttc catactatca catctgcatc ctaaccccac
     1621 cctgcagggt aacatcgctg cggttagcta aatttaccat ggtatcagcg gtaagtttta
     1681 tgacaaaaag attaatttca acaccaaaga taaaggtggg aataagatag cctggcatat
     1741 agctattgac cgattcaaat tccatggtac caatgagtcc atctgctttt actccaagga
     1801 aagtgggatc atcagatgta agctgcccat tcccattgaa atcagtgctg aaaaatccca
     1861 gatttgtagc cagcccaaag ccagtataaa agctgaatag atacatgaca tcaatatatg
     1921 caatagcctg agcgctaagt gtaaacaccg tccagcttac cgtggcacca taggtgctat
     1981 tgagatttgc cgataaaggg taatctatcg aactataagt aactgtaata tcctcatatt
     2041 cagcatcata ttcaccggtt acatccacat tgcccatcat gatatcaccg ccgatactga
     2101 aggtaagacc tccaaatgaa agcaggaatg gcagtatggt tgcttcttta agataattat
     2161 accgtagctt tgcaccaaat gagataaaat taaaatcctg taaacgtgca gtatcagtat
     2221 caatatcagg atcatagata ttttttctga aataaaaaag cttgccaatg atatcaaaat
     2281 tgcctgccag tccaaaacca gcatggacaa cagggtttgc cattacaccc gggaaggtgc
     2341 cgtcttctgc cttatcatca aaatatttgg cattggtcag cgcaacacca agggcaatac
     2401 cagcttccat gtgcggaaaa tgaccaatgg atgatttgcc tattgggtat cctaacatat
     2461 tggccagagc aaaagaattg gcaataaact tgccgttttt atcagttaca tcttgaactt
     2521 gctttcctaa agaatcctca agaactggta tgtcagaaaa atttgcatca gcaggcgtta
     2581 aattaacagt tgtcctggca taggcagcgg tgcatacaaa cacaagtaca atgataataa
     2641 tcttcttcat aacatcctcc gcaatatata ctcttaaaaa aataaaaaga tgtcaaacat
     2701 tattatgaag cttttatatt tttgttatgt atgttcattg ttccctaccg tcattttcga
     2761 cttgacccgg aatcaatttg atgcgtcatt ccagacttcg acaggctgat tgccgagtga
     2821 ttgcatggcg atcatataaa gatgcggtca ccagcgattg ctaagcaatt acatcaaggc
     2881 gcacttaccg aacgcttgct aagcaattgt accgaggcgc ggtcgccgag tgattgcatg
     2941 gcaatcgtat cgaggcgcgg tcgccgagtg attgcatggc aatcgtatcg aggcgccgca
     3001 ttccaaacca gcgattatta tatcacagca atgagttttt cagcagtaac tatcgcccac
     3061 ttatattttt aaaaaaattt tataaaataa taataacact ataaaataat tgacaacgat
     3121 gtgattttgc atgtatactt cattgctggt atgcttacaa ttttgataat cctgaggctg
     3181 tcccatgaca ttgaaaaata aaatcttaat tattttaagt ctttgcctgt tactgtgcgt
     3241 tggtgcgtat ttcatcatat caagcacatt tgccaacttt gaaaagcagc tttttgaaaa
     3301 gtgccgcatt gaggcactgg ttggcgcacg cgttatgagt gaactgattg agatgatgat
     3361 tgacacacgc attcttacta aagaacaggt ttttgaccga aattatattc ccataccaaa
     3421 tacaaatccg cagaagttca gaacaaaata tgacgccata tttgaccgct atattcagaa
     3481 aattgaagac gaatttttga aagatgaaga tctggaattt gcagcacttg ttgatataaa
     3541 cgggtatcta cccactcaca atagcaaata tgctaaacca gaaatgaccg accctgttta
     3601 caatctcaaa tacagccgtt ccaaacgaat atttaatgac atggtaggac tcaatgcatc
     3661 acggtttatt ggcccgggaa cattaaaaca gctgtataac cgtgataccg gtgaaataat
     3721 gtgggacatt tcagcaccgg tgtttgttaa aggtgaacac tttggcgctt tcaggattgg
     3781 tgtttcattg aagcgcataa atgaattgaa aaatcagatg atcattattg tgggtatgac
     3841 tatacttgtc attttaagta taaccatgct catgctattt ttaattttac cgcgaaaact
     3901 ctacgatact gatctggata taccgcagta ttaagccagg cttcgtttca tttttgattt
     3961 atcaatttct atctgtgcat acgcagagtg attgtgtatt gactccatac tttctgtttc
     4021 aacaagaaac cacagcactt ttggatgtgc cagcaatttc acagctacat tgcgcacaat
     4081 atcttcaaca aacacgggat tttcatatga ccgttcagta atatattttt catcttcacg
     4141 cttaagaagc gcatatatct ggcttgaacc acattcttca accagttcaa taagctcttc
     4201 aatccacagt atctccctgg cacgtaccga aacactgaca acacttcgtt gattgtgtgc
     4261 cccatactgg ctaatctctt tggaacatgg gcacactgac agaagtggta cctttacctg
     4321 caatgtgtaa taatcaactg atgaatcgcc ctgcagcgta catatataat ccatcttaga
     4381 tttttcacca ctgactgggg cagtcttggt tatgaaatag gggaattcaa tatttacatg
     4441 ggatgtcttt gcgttgagtt ctttctttaa atcttccagg atgtcacgaa cattttttgt
     4501 gcttatgcca ccctggtaac ggttgagcac ctcaacaaaa cgggacatat gtgtgccacg
     4561 gaaatgttcg ggcaaatcca catacatgtt gatgcgagca acggtatgtt gcaatccatc
     4621 agcacggtca agcaccacaa ccggataggt aatnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnngcagtgc gtgatatgcc gctatcgtac atgatatcaa attcagcctg
     4801 tctgaatggt ggagcaacct tatttttaac aaccttaaca cgtacacggt tgccaacagc
     4861 atcctctcct gcttttaagg tatcaacctt gcgtatatca agccgaactg aagcataaaa
     4921 cttcagcgca ttgccaccgg tggttgtctc tggcgagcca aacataacgc caatcttatg
     4981 acgaatctgg ttaatgaaaa tcacacttgt tttggatttt gatatgacac cggtaagctt
     5041 tcgcagcgcc tggctcataa gacgtgcctg caatcccatg tgagcatcac ccatctcgcc
     5101 ttcaatttcg gcacgcggga ccagcgcagc aaccgagtca atgacaacaa tatctatggc
     5161 acctgagcgc accaacgatt ctgtgatttc aagcgcttcc tcaccggtat ctggctgcga
     5221 cactaaaagc tcgtcggtat tgacgcctaa tttttttgca tataccggat caagagcatg
     5281 ctcagcatca acaaatgcag caataccacc tgccttttgc gcctgggcaa tgacatgcag
     5341 ggtaagcgtg gttttgccgg atgattcagg accataaatt tcggtgatgc gccctcgcgg
     5401 tataccgcca atacccaggg caatgtcaag ctccagtgag cctgttgaaa tagctggcac
     5461 cttcatcttt gcgtgctcat cgccaagccg catgatggag cctttaccaa actgcttttc
     5521 tatctgcaaa atagcggatt ccagcgcctg tgatttttga ttgagttctt tctgttcgct
     5581 catgattatt acctcattgt gtggtttata ccaatatata acgctaattc tgcaaaaaaa
     5641 taacaagtaa tatcaaaaaa acatcaaaaa atttttgtgt gcgatagtta ctgtaaaata
     5701 gcattatgtc atattacaca tagagtgtaa aaaatgtcaa cttatatgta ttttttctct
     5761 tccaccactg tatataaacc tttaggttgt tcccctattt tttatatcat acggaatgaa
     5821 taagacttct cctcacttca tacaaatgag ttaacaaata attattgatg tgatgcaata
     5881 tatgattaaa aaaatgaatg aaaaatgtat tatttacatc gttcccctgt aaaaatccag
     5941 catgtttcag aaccacacgt ttcacacacc caatagtacc catcatcttc acatgatttt
     6001 tgccccacta cacatccaga atacccataa tcaatcaatc ctacaataat gaaggttgca
     6061 acaactaccg aacataccat cataattata cgtgacataa cacgacattc ctccttaaaa
     6121 atattcttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt gtaatactgt
     6241 attgacgaca gtgtcatggg caatggtata aatgcctggc actgatacgg cacatagcca
     6301 aataatgctg caatatcctt acgcatcctg tacacagagc tatcggtact tccaggatgg
     6361 caggtcatca aatacggcac caaataatac tccctgccca ggcgtttgca tgcagcatta
     6421 aacgcattta caaattcctc aagtggaata gcacttactt tacgcatggc tttgagcacc
     6481 gctttatccg tatgttccgg agcaatctta agctgaccgc tgataaaatg cttcaccaga
     6541 aattcaagat tcttcttagc attgctcccc tgcataaaca tatcataccg tatacctgag
     6601 ccaacagaaa ccttctttac attgggcagg gtcagagcat cttccataag attaagccat
     6661 ttatctgttg ccaaccgtaa gtgcttacac acagcaggaa atacacagct ttcacggtta
     6721 catccgccaa tagcacacgt aaacccatac atgtttgccg acggtccgcc aatatcgctt
     6781 atatgcacct tgcgttttga cacagtaact atctcctgct ttattgaatc agccgagcgg
     6841 ctgaccacca tctttccctg atgcagggta agcgaacaaa acgagcagcc acctgcacat
     6901 ccacgatggg catttataga atacttgatc atttcaaacg ccggtatggt atctttatac
     6961 attggatggg gttttcgctc atacggcagt tcataatatg catcaagttc tttcctggtt
     7021 acagcaggcg gtggagtgtg gataatatac ctgccaccac ttttttgtgc tatcaccttg
     7081 gtgccaaaat tctggtaaaa cattttgtac ataacaagca atgattcaac gtttgccagt
     7141 gcatcttctt ctgtaggcag ttcaacagca tcatcaggtt tttgtttggc gatagttact
     7201 gtgccaggaa tcccttgcag gctctgaccc cctgataggc gccgtgctat ctccaaaatc
     7261 tgatattcac ccatgccata cacaaggata tcagcgcggc tatccaccat aatgcttcgg
     7321 cgcagcttgt cctgaacaaa atcataatgg agtagccttc gcattgacgc ttcaatgccg
     7381 ccaatgacta tgggtacatc cttaaaccgc tgttttataa gattacagta ggtaataact
     7441 gcattggatg gcttgatgcc acctttgcca ccaggtatgt aagggtcatc attgcgtatt
     7501 tttttaaacg ccgtaaagcg agaaagcatt gagtcgatat tgccgctcgt tacaccaaaa
     7561 aaaagatttg gcctgccaaa aacttcaact gatgatggat ccaggaaatt gggcattgca
     7621 ataatgccca ctgaaaagcc tgctttttct aaaacacggc caatgactgc aataccaaat
     7681 gacgggtgat caacatatgc atcacccgtc accaggacta tatcaacgtt gttccagctt
     7741 agttttaaag cttcttcttt tgttaccggc aaaaacaatg gtgtatccta caactttgct
     7801 tcaatctttt ctccaatatg gttgcgctgt tttatatatt caagtttatt taacgcatca
     7861 acaaatgaaa ggatacctgc cactgaaata tcaatattga caccattgcc aaatactttg
     7921 agctgtcgcc cgtttatttc ttcaaccact gttaccgtta cctcacacaa tgcatctgat
     7981 ccgccggtga tagcaactaa attgaaggac tcaatctttg cttgcgtacc ggtaatcttt
     8041 ttaacggcat tgatacctgc atcaacacca ccattgccat gtgccacatc aaatgtttca
     8101 acaccatcat tgtagcggtc tttaatagtt accagtgtca ttggtggcga atacatacca
     8161 gttgaaatct gtacattggt aactttgtag cgccctttca tcttgtatac tgcttcacct
     8221 accagcactt ccaggtcttc atcaaatatc tgtttctttt tatcagcaag caccttgaag
     8281 agtttaaaga aacgttccag ttcttcatcg gtgaggttat accctaaact gtttaaccgt
     8341 gcaataactg catggcgacc cgaatgtttg cccagtacca gtgttgattt ggtaattcct
     8401 acatcttcag gtttcattat ttcataggtc attgcgtttt taagtacacc atcctgatgt
     8461 atgcctgatt catgggcaaa cgcattagca ccaacaattg ccttatttgg ctgcacactc
     8521 acccccgtaa tatgcgtcaa aagtttggat gttgacataa tttgctttga gttgatatca
     8581 gtatacacct gaaatttttc accacgtgtt ttcaatgcca ttaccagttc ttccatggca
     8641 gtgttgcctg cacgctcacc aatgccattg atggtacact ccacctgtcg tgcaccagct
     8701 tctatagcag ctaatgcatt tgctgtggca agtcctaagt cattatggca atgcactgat
     8761 atgattgcct tatcaatatt tggtacggta ttaaaaagaa attttataaa ctcatgaaac
     8821 tccgatggaa ttgcataccc caccgtatct ggaatgttca ctgttgtggc accagcttta
     8881 ataactgctt ccaccatctg cgctacatag ctccagtcac ttcgggtggc atccattggg
     8941 gaaaattcaa catttgaggt atattttgca gcatgcttta cagcatcaac agctatcgcc
     9001 agcacctctt cgcgagtctt tttaaactga tattgaatat gaatatctga gcttgaaata
     9061 aaggtatgga tgcgtggatt gtctgcatac ttcaatgcat cccacgccac atcaatgtct
     9121 ttgaagttag cacgtgcaag ccctgccacc tgcactcctt ttactctttc tgatatgcgc
     9181 tttacggctt caaaatcacc cggcgaggat attgggaatc ctgcttcaat aatgtccacc
     9241 cccaatttta caagctggtc agcaaaatga agtttttcat caatattcat actgaagcct
     9301 ggcgcctgct caccatcacg taatgttgta tcaaatataa atactttatt ttgttttgat
     9361 tttgtaactg tcatggcctt tctcctttac ctgtagttga tatcaaaatt aatagcgata
     9421 atttcttggt aataattttt ttagtaatcg ttgggaattc atccctttac acctccagtg
     9481 catataaaat aaaaccgcca cggggtttcc gtggcggcat aacatttcac tataccaata
     9541 cctctcacga aacccctgtt gcggttttcc ccaatagtag tagacctaga aggttagcta
     9601 cgttgtaaag gtttcgtgaa atcataatag atgtcatatc attatataca ttaccattta
     9661 attcaaataa atcacctaat actatgttgt caatatattt taatcattcc ataacaaaat
     9721 tttaatactc taatacttta tttcggcttg aagccagtaa taaaatacac ttttattatt
     9781 cttccataca atggtatcta tgcttgattt tcacagacaa aaaatccata tattgttatc
     9841 ccattcggat actatccacc ttgagtaacc caggatactc cctgcccttc cctgcacttt
     9901 cttttgtcca gacggcaaca actccatagt attcttttca caaggggaaa agggacttca
     9961 ggcgccagcc ccttttcccc ttgccatccc ctttaccccc gcttggcgcc tatcgttt
//
LOCUS       DAORYL010000098         9955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_5102_length_9955_cov_11.105051, whole genome shotgun sequence.
ACCESSION   DAORYL010000098 DAORYL010000000
VERSION     DAORYL010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5102_length_9955_cov_11.105051"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..723)
                     /locus_tag="PLV81_13505"
     CDS             complement(85..723)
                     /locus_tag="PLV81_13505"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HOM11155.1"
                     /translation="MNKKTCVLGATGLVGSNLLLLLTNDTYYSAIHVITRRKVHLQAR
                     SVSQYIADFNNLETVAHAFAVDDVYCCLGTTMKKAGSKEAFKQVDYGYVVKAARIAHA
                     QGAKQFLVISAIGAHPHSLFFYNRIKGQMEKDVASIQFDAVHIFRPSLLLGQRDEKRF
                     LEDIAQKCAKPFQYILKGTLKKYAPVEAKHVAVSMIGAAKSHVPGIHIHEAF"
     gene            complement(773..1261)
                     /locus_tag="PLV81_13510"
     CDS             complement(773..1261)
                     /locus_tag="PLV81_13510"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOM11156.1"
                     /translation="MIVNIIITISGIIFWIILLPVTIALISFKIVPTPHHITDLAGIG
                     VAVGIIGLLISLWAVISQITIGKGTPVPVFGPQKLIVTGPYRFCRNPMTLGLTLYYTG
                     AGIIINHGIAAALLLAIVLFHMLYDALFEEKELIHKFGNDYIHYKKTTPFILPKIRLY
                     KK"
     gene            complement(1347..2048)
                     /locus_tag="PLV81_13515"
     CDS             complement(1347..2048)
                     /locus_tag="PLV81_13515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOM11157.1"
                     /translation="MKKQWFIIISIILLVNIQASVVKQKQADTEKVKQQYVSDVERKD
                     AIIKKIHALMKEINANVGGEEKLVVPVEPSNATDVVDHDEYAKNEMPPDKKPEEDKKG
                     NKESLIAPTKGKMTSKYGYRENPVTKKYSFHKGIDIAAPKGTKVVASAHGIVASSQYM
                     NNGYGNCIIIEHENNIKTLYAHLEKRLVKPGEVVQQGQQIGTVGATGRATGPHLHYEV
                     YKGKQSINPEELIHY"
     gene            complement(2277..>3989)
                     /locus_tag="PLV81_13520"
     CDS             complement(2277..>3989)
                     /locus_tag="PLV81_13520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM11158.1"
                     /translation="DVLISAQMVSLDGIPTSIAIITDISALKEAYDTIQQQYEEIQSQ
                     YEELEAMNEEMVRTHYELTEATHKLEEETERLHATLRSIYDGVVATDLQGHIILANDK
                     AEKLAGKSSDEVIGRPFNSVFTFYYVTTREEFKNPVNEILQYGELDTSGFALTFKSQD
                     RDVVVELSGAPIYVEGNIIGTVIVMHDITGQYWLEKEIQKVSKLESLSILAGGIAHDF
                     NNLLTSIIGNLSILKVKLTDGELYQKYIERIENAAQRAADLTRQLLTFSKGGEPIKEV
                     TSLEQVIRDTAQFVLSGSSVRCNFEIQSDMWNCEVDVGQMSQVFQNLIINAAQSMPHG
                     GIITIKAENFVYDGTQQKPLLPGNYVRITVKDEGIGIPKENLSRIFDPFFTTKERGSG
                     LGLATSYSIIKRHGGHIEVHSEVDKGSKFIIYLHATEKSIHSHTGQEAHITPPGGKIL
                     LMDDDFTIYEVASHMLDMYGFQVDWVDSGEKAIIKYRESIADGFPYDIIIMDLTVPGA
                     MGGVQAVREILEINPKAKVIVSSGYSNDPVMSHYKEYGFKGVIAKPYKIEEIVRVINE
                     VLSQ"
     assembly_gap    3990..4089
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4090..4923)
                     /locus_tag="PLV81_13525"
     CDS             complement(<4090..4923)
                     /locus_tag="PLV81_13525"
                     /inference="COORDINATES: protein motif:HMM:NF019316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HOM11159.1"
                     /translation="MPDKKSLVTLILSIFFLWAIVTEVFASNTLFVTDDFDVANLGLY
                     VQYLRDVPPHTDISEIITAYNNNQFKESTDINPSFGYSHNCIWVSFTLANTSKTPVPL
                     LLEYDYPIIDDITLFIPGGVHFKEIQTGDRFPFSSRPFPYRSFVFPIEVKPGVNTYFL
                     RIQSEGSLVVPLKLWGLKAFEDMRNSTLPLIWMHYGLMVALFFYNLFLFISTREKSFL
                     YVGIFIIGVTLFAMANNGLAAQYLWPASTWWGNICHPFFVFFAMTGILLFTREFLSLR
                     FV"
     gene            5087..5227
                     /locus_tag="PLV81_13530"
     CDS             5087..5227
                     /locus_tag="PLV81_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11160.1"
                     /translation="MAHYKLDGAKFESLEELKEVMWQLYKDKMSREEFEKYVEQNVQV
                     SE"
     gene            complement(5212..5988)
                     /locus_tag="PLV81_13535"
     CDS             complement(5212..5988)
                     /locus_tag="PLV81_13535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOM11161.1"
                     /translation="MDAFPVVFSDIDGTILDYTTYSYKKSLPAIQKLAGRGIPLILVS
                     SKTYAEMLKLHKELSLTYPFVFENGAGIAKPDGSYILCGKSYDQLLPYKTIIEQLCGN
                     VQWADSLSLQELSAYTGLTIQETKNMMARMASVLCIAPAPLDIERINNELIPHGIAIT
                     TGGRFFTVIDSTVNKGNAVHVIKAMYREKHGAIYSYAIGDGINDADMFNAVDEAYFVG
                     RKDLWGTIQKLCPVIHKTKKTGPSGFSEVIKNIIKNYSLT"
     gene            complement(5991..8615)
                     /locus_tag="PLV81_13540"
     CDS             complement(5991..8615)
                     /locus_tag="PLV81_13540"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOM11162.1"
                     /translation="MKIRVVFTAILFISSVTMVYAASDGTALMLLGGSASMFGRADTG
                     VAYLGSDTFFINPAAIAKEERFIGSFNYGNLLFDYYNPSVVAGFPTSYGVIGGAFRLV
                     SGSDTLNGYQFVLGGGKEFTRRLSMGASLSFLLGNGPNSGAYFAGITLGSMYQTTINT
                     DIGKGFGLYYPTIGIAINAGLPMGSDRDFADMNQVTVGYTLPLYKRNDIQVSLLNDYT
                     FVQYDEIAVKVGTEGVYKNTYIVRLGFMAPGPYEYADFTAGAGYRLTGQEYQAEFNYA
                     LVHYKKTDFVHYVGTQVRYGRLDREPPQTAVKASQRYISPNTDGVQDFVLFTINVSDA
                     SKIGGWRLQIKNDAGTVVKEFRVSDRDVEESLTLKSFIQKIWTPKESAVVPETIFWDG
                     TDANANVLPDGDYVYAFMAWDDRDNISPEVTGVITIDNTPPVVELAVEDRFFSPNNDG
                     RKDTLKIVQTIKTQSNDVWYATFVDKEGKIVKKYSWQGTEVPKTLVWDGKDDAGNDVP
                     EGLYMYQIQCTDNAGNKAQAMIREISLTRQYQTVDVSASLEYFSHALHNTIVFTPIVS
                     DTKDLVKWQLVVTNEKDKPVYQTQGQVMPKTITWDCKTDKGEKLNDGIYRYKFSAEYL
                     SGNNPQSFVKTFTIDSTPPDVNLSYEPDLFSPDGDGENDILTITPSINDNVGIKSWEL
                     IIYAPAGYVFKTFKGTEVPGELKWDGIGVNNELVESAADYFMELSATDITGNVAKTKR
                     IKLPIDVLVMVTERGLKIRISNIEFAFDSAKLTGKAFPILNRVAEILNKYEKYNVLIE
                     GHTDDIGEEQYNLKLSEARAQSVMDYLVKKGIDKKRLSSRGMGETSPFLPNTSVENRR
                     RNRRVEFLLIKPEESQVN"
     gene            8811..9284
                     /gene="bcp"
                     /locus_tag="PLV81_13545"
     CDS             8811..9284
                     /gene="bcp"
                     /locus_tag="PLV81_13545"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015182001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOM11163.1"
                     /translation="MNEDIILNKPAPQFKLPDKDKNMVDSNNFRGTWVVVYFYPKDNT
                     QGCTLEAIDFTKSIREFNNYNAVVVGISPDSPESHCRFYDKHNLNLILLSDTDHKVAK
                     EYGVWKKKSMYGKSFMGIERSTFLIDPEGRVRAVWRKVKVPGHVEEVLQTLKSFQ"
     gene            9296..9850
                     /locus_tag="PLV81_13550"
     CDS             9296..9850
                     /locus_tag="PLV81_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOM11164.1"
                     /translation="MKLQEAIEARRAFRSLAPVTITETMVSELAKAASLAPSCYNNQP
                     WRFVFVYEPGKLDEVKKALAKGNEWARKASMIIAVFSKEKDDCVLKNRIYHHFDTGMA
                     TALMLLKATELGLVAHPIAGYDEEAVKKACAIPDEFTVITLIIIGKKDGIIHDDLTEW
                     QKESEKSRPPRKPLEEFAFHNVYK"
BASE COUNT         2954 a         1919 c         1816 g         3166 t
ORIGIN      
        1 agttggcatg tgttaatgtc aaggtgtaat aaagtgttta cattctttat ccaatgacca
       61 gttaccctat ccagtaacta tcgcttaaaa tgcttcatgt atatgaatac ctggtacatg
      121 cgattttgct gccccaatca tggatacagc aacatgtttt gcttctacgg gcgcgtactt
      181 ttttaacgtc cctttcaata tgtattgaaa tggttttgca catttttgag ctatatcttc
      241 aagaaagcgc ttttcatccc tctgacccaa taaaagtgat ggcctgaata tatgcactgc
      301 atcaaattgg attgaagcca cgtccttttc catctgccct tttattctat tgtaaaaaaa
      361 caatgaatgg ggatgtgctc caatagcgct gataaccaaa aattgttttg ctccctgtgc
      421 atgggcaatt ctggctgctt ttactacata gccataatca acctgtttaa atgcttcttt
      481 tgagcctgct ttcttcatgg tagtacccag gcaacaatag acatcatcca ccgcaaacgc
      541 atgggctacc gtttcaagat tattaaaatc ggcaatatat tgagaaactg accttgcctg
      601 tagatgtacc tttcgccgtg ttatcacatg tatagcagaa taataagtat cattagtgag
      661 caacaataga aggtttgaac ctacaagacc agttgcaccc agcacacacg tttttttatt
      721 cataatagta atgttaaaaa aattttgtaa taaagtcaac tgaaaataaa aattattttt
      781 tatacaaacg tatttttggc aggataaaag gagtggtctt tttatagtgt atatagtcat
      841 taccaaattt gtgtatcagt tctttttctt caaaaagtgc atcatagagc atatgaaaaa
      901 gaactattgc taatagtagt gctgcagcta ttccatgatt aataataatt cctgcacctg
      961 tataatacag tgtcaggccc agtgtcatgg gattgcggca aaacctgtat ggtcctgtaa
     1021 caataagttt ctgagggcca aacacaggga caggtgttcc tttacctatg gttatttgtg
     1081 agataacagc ccatagagaa attaacaggc caatgatccc cactgcaaca cctatacctg
     1141 caaggtcagt tatgtgatgc ggtgttggta caattttaaa tgatattagt gcgatagtta
     1201 ccggtagtag tataatccag aaaataatcc cggatattgt tataataatg ttaacaatca
     1261 cctctcataa tcctttcaat aaaaaatctt caggcataaa aatgtatttc ctttgaacat
     1321 gtgagatagt aatcataagt aaaaagttaa tagtgaataa gctcttctgg atttattgac
     1381 tgcttgcctt tatacacctc atagtgaaga tgtggtccgg ttgctctacc ggtagcacca
     1441 acggtgccta tctgttgacc ttgttgcaca acctcacctg gctttaccag acgtttttca
     1501 agatgtgcat acagtgtttt tatattgttt tcatgttcaa tgatgatgca attaccatat
     1561 ccattattca tgtactgtga tgaagcaaca atgccatgtg cacttgccac tactttagtc
     1621 ccttttggtg cagctatgtc tatcccttta tgaaatgaat attttttggt tacaggattt
     1681 tccctgtatc catatttact tgtcattttt ccttttgttg gtgcaattaa ggattctttg
     1741 ttgccttttt tgtcttcttc tggttttttg tcaggtggca tttcattttt tgcatattca
     1801 tcatggtcaa ccacatcagt agcgttagaa ggctcaacag gaactaccag tttttcttct
     1861 ccaccaacat ttgcatttat ttctttcatt aatgcatgta tttttttaat gattgcatcc
     1921 tttctttcaa catcagatac atactgctgt tttacttttt cagtgtcagc ctgtttttgc
     1981 ttaactacag aagcctgaat atttaccaat agtattatgc ttataataat gaaccattgt
     2041 tttttcatat atacattaaa tctccttgta aaatagaatt tcatgattgt catccctgat
     2101 actatccgta atctttggag cagtaagatt gtgaatcacg ttcaggatga ccattgcaaa
     2161 aaaatatttt catttcacga ttgcagagta acaccctgta tccagataca gttatctgct
     2221 atcacggcga tatatattca tactatattt attttcggag ttattaaagg aaagtcttac
     2281 tgtgaaagga cttcattaat tactcttact atctcttcaa ttttatatgg ttttgcaata
     2341 acacctttga atccatattc tttataatgt gacatcaccg ggtcattgga atatccgctt
     2401 gaaacaataa cttttgcttt aggatttatt tctaaaattt ctcttactgc ctgtacccca
     2461 cccatggctc cagggactgt aaggtccata atgataatat cataaggaaa gccatcagct
     2521 atggattcac ggtacttaat aattgctttt tccccactat caacccaatc aacttgaaat
     2581 ccatacatat caagcatatg cgaagcaacc tcatagattg tgaaatcatc atccatgagc
     2641 agtatttttc ctcctggggg agtaatgtgt gcttcctgtc ctgtgtggct atgtatgctt
     2701 ttttctgtag catggagata gataataaat tttgatcctt tatctacttc tgaatgaact
     2761 tcaatatgtc caccatgacg ctttattata gaataacttg ttgccagccc taaaccactg
     2821 ccacgttctt tagtggtaaa aaaaggatca aatatccgtg ataaattttc cttcgggata
     2881 ccaattcctt cgtcttttac tgttatcctg acataattgc caggcaacag tggtttttgc
     2941 tgagtaccat catacacaaa attttcagct tttatggtaa taatccctcc gtgaggcatg
     3001 gattgtgctg catttatgat taaattttga aacacctgac tcatctggcc cacatctact
     3061 tcacaattcc acatatcaga ttgtatttca aaattacacc ttacactgga acctgacagt
     3121 acaaactgtg cggtatccct gattacctgt tccagcgaag ttacttcttt tattggctca
     3181 ccaccttttg aaaaggttaa aagctgtctg gtgaggtcag ctgccctttg tgctgcattt
     3241 tcaatgcgtt ctatatactt ttgatataat tctccatcag taagtttaac tttcagtatt
     3301 gaaagattgc caattataga tgttaataga ttattgaagt catgagcaat gcccccagct
     3361 aaaatagaaa gtgattctaa tttacttacc ttttgaattt ctttttccag ccagtactga
     3421 ccggtgatgt catgcattac aataactgta ccaatgatat ttccttcaac gtagattgga
     3481 gcacccgata gttctacaac tacgtctcta tcctgggatt taaatgtcag agcaaagccg
     3541 cttgtatcaa gctcaccata ttgtaaaatc tcatttacgg gatttttgaa ttcttctctg
     3601 gtggtaacat aataaaatgt gaatactgaa ttgaatggcc tgccaattac ctcatcagat
     3661 gatttgcctg ctaatttttc agccttgtca ttggcaagaa taatatggcc ctgtagatca
     3721 gttgctacaa caccatcata tatagaacgc aatgttgcgt gtaatcgctc agtctcttct
     3781 tcaagcttat gcgtggcttc agtaagttca taatgcgtgc gcaccatttc ttcattcatt
     3841 gcttccaatt cttcatactg agattgtatt tcctcatact gctgttgtat ggtatcatac
     3901 gcttctttca atgcgctaat atcagtgata atggctatgc tggtgggtat gccatccagg
     3961 gaaaccatct gagctgaaat gaggacatcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnna acaaatcgta atgataaaaa ttcacgggta aagagcagta tgccagtcat
     4141 agcaaaaaat acaaaaaatg gatggcatat attcccccac catgtacttg ctggccacaa
     4201 atattgagcg gcaagtccat tgttggccat ggcaaaaagg gttacgccaa tgataaatat
     4261 acctacatac aaaaagcttt tttcacgagt ggatataaat aaaaatagat tataaaaaaa
     4321 taatgcaacc atcagaccat agtgcatcca tattagtggt aatgtactat tgcgcatgtc
     4381 ttcaaaagct tttaatcccc acagttttaa gggtacaacc agggatcctt cagattgtat
     4441 tcgaagaaaa tatgtgttaa caccaggttt tacttcaatg gggaatacaa atgatctgta
     4501 aggaaaagga cgtgatgaaa atgggaaacg atcacctgtt tggatttctt taaaatgcac
     4561 accaccggga ataaaaaggg taatatcatc aataatgggg taatcatact ctaaaagcaa
     4621 tggtacaggt gtttttgatg tatttgcaag ggtaaagctt acccatatac agttgtgaga
     4681 atagccaaac gatggattta tatcagtaga ttctttaaat tgattattgt tataagcagt
     4741 gataatttcg ctaatatcag tgtgtggggg tacatcgcgt aaatattgta catacaatcc
     4801 aagattggca acatcaaaat catcagtaac aaatagtgta ttactggcaa aaacttcggt
     4861 aactatcgcc cacaaaaaaa atatggataa tatcaatgtt actaagctct ttttatctgg
     4921 cattgttgta caccctgcaa acgatgatac tggtatttgt gatttaattc aaatgttttt
     4981 ttaggataat tgtaacatta tggtgtaaaa ttttatattg acagtatagt tgtaaagtgt
     5041 ttattggtat ttaagatttg tatattcagc aaaagggttc tgtagtatgg cacactataa
     5101 gcttgacggg gcaaaatttg aatcgcttga agaattaaaa gaagtcatgt ggcagttgta
     5161 taaggataaa atgtcccgtg aagaatttga aaagtatgtt gaacagaatg ttcaggttag
     5221 cgaataattt tttattatat tttttatcac ctcactaaaa ccagatggtc cggttttttt
     5281 tgttttatgt attacagggc ataatttctg aatggttccc cacagatctt ttcttccaac
     5341 aaaataggct tcatcaacag cattaaacat gtctgcatca ttgatgccat cgcctatcgc
     5401 ataggagtat attgcaccat gcttttcccg gtacattgct tttatcacat gaactgcgtt
     5461 gcctttatta acagtgctat caataacagt aaaaaacctc ccaccggttg ttatggctat
     5521 accatgcggg ataagttcgt tgttgattct ttcaatatca agtggggcag gtgctataca
     5581 taacaccgat gccatgcgtg ccatcatatt ctttgtttct tgtatggtaa gcccggtata
     5641 tgccgatagt tcctgcaatg aaaggctatc tgcccactgg acattaccac acaactgttc
     5701 aataatcgtt ttatacggga gaagttggtc atatgatttt ccacataaaa tataacttcc
     5761 atctggtttg gcaataccag cgccattttc aaagacaaat ggatatgtaa gcgaaagctc
     5821 tttgtgcagc ttcaacattt cagcatacgt tttgctggaa actaaaataa gagggatgcc
     5881 cctgccggca agcttttgaa ttgctggcag ggatttttta tatgaatatg tggtgtaatc
     5941 cagtatggtc ccgtctatat cagaaaatac aacagggaaa gcatccatgg ttaattaacc
     6001 tgtgactctt caggtttaat caacagaaac tcaacacggc ggtttcgcct tcggttttca
     6061 acacttgtgt taggtaaaaa tggtgatgtt tcccccatgc cgcgtgaaga taagcgcttt
     6121 ttatcaatac cttttttgac cagataatcc ataaccgatt gagctcgcgc ttcagaaagt
     6181 ttcagattat actgttcctc acctatatcg tcagtatgcc cttcaatcag aacattgtat
     6241 ttctcgtatt tgttgaggat ttctgcaacc ctgtttaaaa ttgggaatgc tttcccggtt
     6301 agttttgcac tatcaaacgc aaattcaatg ttgctgatgc gaatcttgag tccacgctct
     6361 gtcaccataa ccaatacatc aatgggtagt tttatgcgtt ttgtctttgc aacatttcca
     6421 gttatatcgg tggccgatag ttccatgaag taatccgcag cagattcaac aagctcattg
     6481 tttaccccaa tgccatccca cttgagttct cccggcactt cagtgccttt gaatgtttta
     6541 aatacatatc cagctggtgc atagattatc aattcccagc ttttgattcc aacattatcg
     6601 ttaatagatg gggtgatagt aagaatatca ttttccccat caccatcagg cgagaaaaga
     6661 tcaggttcat aggatagatt aacatcaggt ggagtgctat caatggtaaa cgttttcaca
     6721 aatgattgtg gattattgcc acttaaatat tcagcactga atttgtagcg atagatgcca
     6781 tcattaagct tttcgccttt atctgtcttg cagtcccagg ttattgtttt tggcatcacc
     6841 tgaccctgag tctgatatac aggtttgtcc ttttcattgg taacaacaag ttgccattta
     6901 acaaggtctt tggtgtcaga gactatagga gtaaatacta tagtattgtg taatgcatgt
     6961 gaaaaatatt ccagtgatgc gctgacatca actgtctggt actggcgagt taaacttatt
     7021 tccctgatca tagcctgtgc tttgttgcca gcattgtcag tgcactggat ctgatacata
     7081 tacagacctt caggtacatc attaccggca tcatctttac catcccatac taatgtttta
     7141 ggtacttcag tgccctgcca gctgtacttt ttcactattt taccttcttt atcgacaaat
     7201 gttgcatacc aaacatcatt actctgagtt ttgatagttt gaacaatctt taatgtatcc
     7261 tttcgcccat cgttatttgg tgaaaagaat cgatcttcaa cagctaattc aacaacagga
     7321 ggagtattat ctatagtaat aaccccggta acttctggtg atatgttatc tcgatcgtcc
     7381 catgccataa atgcatacac atagtcacca tcgggcaaca catttgcatt tgcgtcagta
     7441 ccatcccaga aaatggtttc aggaaccaca gcagattctt ttggtgtcca aattttttgt
     7501 ataaagcttt ttaatgtcag cgattcttca acatctctat cgcttaccct gaattccttt
     7561 actacggttc ctgcatcatt tttgatctgt aatcgccaac cgccaatctt acttgcatcg
     7621 ctcacattta tagtaaacag tacaaaatcc tgaacaccat cagtattggg agatatatac
     7681 cgctgacttg cctttacagc agtttgtggt ggttccctat ctaacctgcc ataacgtact
     7741 tgagtgccaa cgtaatggac aaaatcagtt tttttataat gtaccagagc gtagttgaat
     7801 tcggcctggt attcctgacc ggtgagcctg taaccggcgc ctgccgtgaa atcagcatat
     7861 tcgtacggcc cgggtgccat aaaacccaac cgcacaatat atgtattttt ataaacacct
     7921 tcagtaccaa cctttactgc gatttcatca tattgcacaa aggtatagtc attgagcagg
     7981 ctaacctgta tgtcatttct tttatacagt gggagtgtat agccaacggt tacctgattc
     8041 atgtcggcaa aatccctgtc acttcccatg ggtaaccctg cgttaatagc aataccaatg
     8101 gttgggtaat ataagccaaa acctttgcca atatcggtat tgatggtggt ttgatacatg
     8161 ctgccaagag tgatacctgc aaaatatgca cctgagttgg ggccattccc caaaagaaat
     8221 gacagcgatg cacccattga aagccgccgt gtaaattctt taccaccacc cagcacaaac
     8281 tgatagccat tgagggtgtc agagccagat acaagcctga atgcgccacc aatgacacca
     8341 tatgaggttg ggaatcctgc aacaactgat gggttgtagt aatcaaaaag gagattgcca
     8401 tagttaaatg acccaatgaa acgctcttct tttgcaattg ctgccgggtt tataaagaag
     8461 gtgtcagagc ctaagtaggc aacaccggta tcagctcttc caaacatgct ggcacttcct
     8521 ccaagcaaca tcagcgcagt gccatcagaa gcagcatata ccatggtgac agagctaata
     8581 aaaagtatag ctgtaaaaac aacacgaatt ttcatgatta tcctcctgtt aagtgcggat
     8641 ctttttatat ttttttaaca ttcattgcat ccaaaatatt ttttgtcaag tggttatatt
     8701 tttaagtggg gcaattgaca aagatcaaaa taattagtat tttagaatat actttatgat
     8761 tattgagctg atttcaatat aaatgaatat tttttaatga ggatagagtg atgaacgaag
     8821 atattatatt aaacaaacct gcaccccaat tcaaacttcc tgataaagat aaaaatatgg
     8881 tagatagcaa taatttcaga ggcacgtggg tggttgttta tttttatcca aaagataata
     8941 ctcaaggatg taccctggaa gctatcgatt ttaccaaaag tataagggag tttaacaatt
     9001 ataatgcagt tgttgtaggc attagcccgg atagtcctga aagtcattgc agattttatg
     9061 ataaacacaa ccttaatctg attctcttat cagatactga tcataaagta gcaaaagaat
     9121 atggggtatg gaaaaaaaag agtatgtatg gcaaaagctt tatgggtata gagcgaagta
     9181 cattcctgat tgatcctgaa gggagggtca gagcagtatg gcgaaaggtt aaggtgccag
     9241 gacatgttga agaagtgttg caaacactta aatcttttca gtagaggagg ttactatgaa
     9301 attacaggaa gctattgaag cacgaagggc attcaggtcg cttgctccgg tgacaattac
     9361 tgaaacaatg gtgtcagagc ttgcaaaagc ggcatctctt gctccatcat gctataacaa
     9421 tcaaccgtgg aggtttgtat ttgtctatga gccaggcaaa ctggatgaag taaaaaaagc
     9481 cctggcaaaa gggaatgaat gggctcgtaa ggcttccatg attattgctg ttttcagcaa
     9541 ggaaaaggat gattgtgtat tgaaaaatcg tatatatcac cattttgata ctggaatggc
     9601 tacagcactt atgttactta aagcaactga attagggttg gtagctcatc ccattgcagg
     9661 ctacgatgaa gaagcagtta aaaaagcttg tgctatacct gatgagttta ctgtaattac
     9721 cctaattatc ataggtaaaa aagatggtat aatacatgat gatttgaccg aatggcagaa
     9781 ggaaagcgaa aagagccgtc caccaagaaa gccactggaa gaatttgctt ttcacaatgt
     9841 ttataagtaa agggtcagag caaaagctca ccacggcaat aagcacacag acgttcaata
     9901 cactcttcca atgtgttacc cagctctaaa aaatattgat gcagggtaat cttta
//
LOCUS       DAORYL010000099         9692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_5250_length_9692_cov_12.356854, whole genome shotgun sequence.
ACCESSION   DAORYL010000099 DAORYL010000000
VERSION     DAORYL010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9692
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5250_length_9692_cov_12.356854"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..2221
                     /locus_tag="PLV81_13555"
     CDS             53..2221
                     /locus_tag="PLV81_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOM11165.1"
                     /translation="MKRFIKNIASEREYVITREKEMPVSQYYGEDTFNFKVMRQKLPA
                     NVCKKLMRIIKEGERLDPEIADTVAHAMKEWAIEKGATHFCHWFQPMTGATAEKHDSF
                     LEFTEFGEAIERFSAETLVKGEPDASSFPSGGLRSTFEARGYTAWDPSSPAFILRSGI
                     GATLCIPSVFLSYNGEALDKKTPLLRSINAINKSALRVMRLLGNDRATRVIATVGPEQ
                     EYFLIDMDYYYKRPDLVLTGRTLLGATPPKGQQLEDHYFGSIKERILSYMHDVEEELY
                     KLGIPAKTRHNEVAPSQYEIAPIFEKANIAVDHNHLVMETLKRVAQNHRLAALLHEKP
                     FAGINGSGKHCNWSLSDNLGNNLLNPGKSPQDNVQFLVFLTATIRAVYYHADLLRASV
                     ASSGNDHRLGANEAPPAIISVFLGEQLTKILNDIENGIITKATNEAIIDLGLSTLPVL
                     SKDNTDRNRTSPFAFTGNKFEFRAVGSSQSIAFPVTILNTIVAESLDILADKIETKSG
                     DVRQAAIDVIRDEIKKIKPILFMGDNYSREWEEEAARRGLPNKKVTVESLPDLVTKKA
                     ITLFQSYNVLSPVELEARYAIRLERYIKDMDIEAKTMLDMVRTRIIPATMRYQSSVAS
                     SCLRIIDLLGEKAPVDAQKKMCTTITALNNDVIKEASKLDELIEKASTIHDELEKAKF
                     YAYTIKEQMGKLRAKADMLENHTSDNLWPFPRFWEMLFIS"
     gene            complement(2283..3590)
                     /locus_tag="PLV81_13560"
     CDS             complement(2283..3590)
                     /locus_tag="PLV81_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006918029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOM11166.1"
                     /translation="MKAKSTLGIVFFPAFDWAIHPLHPEREERLLYTLDQLNEEGVFD
                     IDGIYQYKPDIASLNDIMRTHFCFPDVSKVTTESHLISAGGTIKAATLVMDNIVNKSF
                     ALVRPPGHHAMKVVHGARGFCNINIEAVMVEYIREHYPVKKIAIVDTDCHHGDGTQDI
                     YWHDRDVLYISMHQDGRTLYPGTGAIHELGGPNALGTTINIPLPPNTSDEGFLIAMER
                     VVLPILKDFKPDLIINSAGQDNHYSDPITNMNFSALGYAKLTEMLNADIAVLEGGYSI
                     QGALPYVNLGITLAMAGLDYSYVHEPDYSQKDLTQDKEITKYIKRVCDQVMKRYEECK
                     KPSFKLMPGNYDVRKKEIYYDTDNIREMQLESIMVCEECRGVLKIETTSTVNPLCLGI
                     EIPLDACDRCQKEGYRLLDTALSGDRYKYVQLIDRKNKKYVLY"
     gene            complement(3587..5257)
                     /locus_tag="PLV81_13565"
     CDS             complement(3587..5257)
                     /locus_tag="PLV81_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011791981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HOM11167.1"
                     /translation="MYLGLDVGGTHTDAVIIDKSGVVAHTKVVTTPHNLLECISQAFK
                     NILTGIDPSHIKRVNLSTTLTTNAIIENKCEEVGVLVSGGPGINPALYSMGNHYYVID
                     GQIDHRGTEITLLNLNQLEKATKKCRKKGIKAFAVVSKFSTRNPVHEQYIQQKLDDAD
                     FISVGHTLSGNLNFPRRIATAYYNAAVWRLYNKFSYAIEEVLRELGVIAPVNILKADG
                     GTIPLHVSRTIPVESILSGPAASVIGTMALCNITEDAVMLDIGGTSCDIAMFADGAPL
                     IEKDGIRLNTHNTLVRSLKTKSIGIGGDSAIKIDGNTVTAGPQRLGMSMAEGGQNPTI
                     IDALNVIGTINLKDTGRSREGMYALEKKSGISSHNIAQQAITYALDIIYNEVMAMIDE
                     VNSRPVYTIHEFLKGKVLKPKTIYLIGGPAKTLAHHITQRFGWNVKVPQYAEVANAIG
                     AAVARTTVDIELFADTQRCRLLVPNLNVNIRIPQEYDVDNAIEDATNYMMQYLESIGV
                     DEREREIEILQVYSFNMVEGFETTGKNIRVHCQVKPGILHQCRDILKI"
     gene            complement(5263..6093)
                     /locus_tag="PLV81_13570"
     CDS             complement(5263..6093)
                     /locus_tag="PLV81_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HOM11168.1"
                     /translation="MKWVFTVLCAVCVGATAYAQELAPIPPLQAHVNDYADILSPTTK
                     TELEAKLTQLEKDDSTQVVVATVPSIGNEDIESYSIRLANTWKIGQKGLDNGVIVLVA
                     RDNRKVRIEVGKGLEETLTDLLAGRIIDYIIIPEFKNGDYDAGITKAVDAIIDIVKGK
                     FDASSLKPQKSAPDYGAFMWIAFIVMTFISALGRLMSGILGAIVLPIIGAFFGFGVPA
                     LVLLAFIGFIGGIIIASMPTGGGGFSSGGGFSSGGFSSGGGFSGGGGSFGGGGASGSW
                     "
     gene            complement(6093..6749)
                     /locus_tag="PLV81_13575"
     CDS             complement(6093..6749)
                     /locus_tag="PLV81_13575"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOM11169.1"
                     /translation="MKDLVSKILSQADAQCIIDAVKKAEKLTSAEIVPMIVSASYHYP
                     LTRIYASAVLSLPLAIALTYYIAPLFYFHKDNLWIFIVFFSLLFIVFKVTIARSVFLT
                     RLFLNPREVDAEVREAAITAFYKNGLYKTADKNGVLVYITLLEKKVWILADEDAHKKI
                     EPEFWETCVKHIITGIKQKQFIPALCATIETIGNRLAKEYPYKPDDVDELKNIIVGDN
                     "
     gene            complement(6763..7356)
                     /locus_tag="PLV81_13580"
     CDS             complement(6763..7356)
                     /locus_tag="PLV81_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOM11170.1"
                     /translation="MKKISYSLAVIIALSLFSFTGCGYNKLQMQEEEVFKAYGDLESA
                     LQRRADLIPNLVATVKGYAAHEKTTLQAVIEARAKATSVQLSPKNLSNPEAIAKFQAA
                     QGELSSALARLMVVIERYPNLKADAHFTDLMHQLEGTENRINVARQRYNEAVKEFNSS
                     IRIFPYNITNSLLLHLERKEYFKAEEGAKTAPKVSFE"
     gene            7605..7964
                     /locus_tag="PLV81_13585"
     CDS             7605..7964
                     /locus_tag="PLV81_13585"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOM11171.1"
                     /translation="MKQSSVEERKILLDAELKKIVEVLKQEYDPEKIILFGSLLDGNI
                     HEYSDIDLLIIKKTSKRPIDRILEVAQIINPSLGVDLFVYTPEEFDTLVNEKYTFFTN
                     ILKNSKILYEKRNSRVD"
     gene            7936..8205
                     /locus_tag="PLV81_13590"
     CDS             7936..8205
                     /locus_tag="PLV81_13590"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOM11172.1"
                     /translation="MKKETAEWISIASEELMAAKHLEKLNLHRMVCYHCQQCVEKAMK
                     AFLVEKDKEFQRTHNILDLKNMVVASGVDIPLTNDEAVFLKNNIL"
     gene            8469..9176
                     /locus_tag="PLV81_13595"
     CDS             8469..9176
                     /locus_tag="PLV81_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11173.1"
                     /translation="MSMHLSADVYEIFEKAFQGKNEAKKVMYAIEEAIVSTVHDTWYK
                     TKEELKLEVLSFYATKDDLLQVRTELLAEMKKDKAEILGIMKQDKAELSGIIEQNKAE
                     LLGIMKQDKAELSGKLEALYEKTERDKSELLGIMKQDKAELLGIMKQDKAELLGIIEQ
                     NKAELLGKFDALFQKTEKDKAELLGIMKQDKAEFALKLEKLDKKFSMYFAVMMFAIIF
                     LNQNALEFLAKILGLIR"
BASE COUNT         3052 a         1841 c         2003 g         2796 t
ORIGIN      
        1 gcagctatta ttaaattata gattaataca aatcaaagag aggatctttt atatgaaacg
       61 ttttataaag aatattgcat ctgagcgtga gtatgtgata acaagggaaa aggaaatgcc
      121 tgtttcccag tattatggtg aggatacctt taattttaaa gtaatgcgtc agaagttacc
      181 tgcaaatgta tgcaaaaaac taatgcgaat tataaaggaa ggtgaaagat tagatcccga
      241 aattgcagat actgttgcac atgcaatgaa agaatgggcc atagaaaaag gtgccacaca
      301 tttttgccac tggtttcagc caatgaccgg agcaacggca gaaaagcatg attcattttt
      361 agaatttact gaatttggtg aagcaattga gcgcttcagt gcagaaacac tggtgaaagg
      421 tgagcctgat gcttcaagtt ttccaagcgg tgggttgcgc tcaacgtttg aagcccgagg
      481 ttacacggcg tgggatcctt caagccctgc atttattttg cgaagtggca taggggctac
      541 attgtgtatt ccttcggttt ttctttcata caatggggaa gcgctggata agaagacacc
      601 actccttcgt tcaattaatg caattaataa aagcgcactc agagttatgc gccttctagg
      661 caacgatagg gcaaccagag ttattgcaac tgtaggtcct gaacaggaat actttcttat
      721 tgatatggat tattactata aacgtccgga tctggtttta acaggaagga cattgttggg
      781 ggcaacacca ccaaaaggac aacagttaga agaccattac tttggtagca tcaaagaaag
      841 aatattatcg tatatgcatg atgttgaaga agaactgtat aagctgggca taccagcaaa
      901 aacacgccac aatgaggttg caccaagtca gtatgaaata gcaccaatat ttgaaaaggc
      961 aaatattgcg gttgaccaca atcatttagt aatggaaaca cttaaacgtg ttgcgcaaaa
     1021 tcatcgttta gctgcgctgc ttcatgaaaa accgtttgca ggcataaatg gttcgggcaa
     1081 gcactgtaac tggtctttgt cagataactt aggcaataac ctgcttaatc ctgggaaatc
     1141 acctcaggac aatgtacagt ttttggtatt tttaactgca accatacgtg cggtgtacta
     1201 tcatgcagat ttattacgtg cgtcagttgc ttccagtggc aatgatcata gattaggggc
     1261 taatgaagca ccaccggcaa ttatctcagt gtttttaggt gagcagctta caaaaatact
     1321 taatgatatt gaaaatggca tcataacaaa agctaccaat gaagctatta ttgatttagg
     1381 gctttcaact ctaccggtac tcagcaaaga taatactgac cgtaaccgta cctcaccatt
     1441 tgcatttact ggcaacaagt ttgaattcag ggctgttggg tcttctcaat ctattgcatt
     1501 tccggttact atcctgaata ctattgttgc tgaaagcctg gatatactgg cagataaaat
     1561 tgaaaccaaa agtggtgatg tacgtcaggc agcaatcgat gtgatccgtg atgagattaa
     1621 aaaaataaaa cctattcttt tcatgggcga caattactca agagaatggg aagaggaagc
     1681 agcacgaaga ggattgccta acaaaaaagt aacagtagag tcattgcctg atttagttac
     1741 aaaaaaagcc ataacattgt ttcagagcta taatgtatta tcacctgttg aacttgaagc
     1801 acggtatgct atacgccttg agcgctacat aaaagatatg gacattgaag caaagactat
     1861 gcttgatatg gtacgcacca gaattatacc tgcaaccatg cggtatcaaa gttcagttgc
     1921 gtcatcatgt ttgcggataa ttgatctgct aggagaaaag gcaccggttg atgctcagaa
     1981 aaaaatgtgt acaacaataa ccgcactcaa caatgatgta ataaaagaag ccagtaagct
     2041 ggatgagctc attgaaaaag ccagcactat ccatgatgaa cttgaaaaag caaaattcta
     2101 tgcctatacc ataaaagaac agatggggaa actacgagcc aaagccgata tgctggaaaa
     2161 tcatacaagt gataatctgt ggccattccc acgattctgg gagatgctgt ttatatcata
     2221 aagatttgct ggaatggatg tatttcctgc aggggtgtat gatcatatgc ccctgcaatt
     2281 atctaataca gcacatattt tttatttttt ctgtcaataa gttgcacata tttataccgg
     2341 tcacctgaca gtgcagtgtc aagcaaacgg tatccttctt tctggcatct gtcacatgca
     2401 tcaagcggta tttcaatacc cagacataat ggattaacgg ttgaagttgt ttcaattttc
     2461 agcactccac ggcattcttc gcacaccatg atagattcta actgcatctc acggatatta
     2521 tcagtgtcat aataaatttc ttttttgcgc acatcgtaat tgcctggcat aagtttaaat
     2581 gaaggctttt tacattcttc atatcgcttc attacctgat cgcaaacccg ttttatgtat
     2641 ttagttattt ccttatcctg agtaaggtct ttttgcgaat aatcaggctc atgcacatat
     2701 gaataatcca gccctgccat ggcaagcgtt atgcccagat tgacgtatgg caatgctccc
     2761 tgaatggaat acccgccttc aagtactgca atatcagcat tgagcatttc agttaattta
     2821 gcatacccca gtgcagaaaa attcatgttg gtgatagggt ccgagtaatg gttgtcctgc
     2881 cctgcggaat taattataag atcaggctta aaatctttta aaatgggaag caccacacgc
     2941 tccattgcta ttaaaaatcc ttcgtcagag gtgttgggtg gtaatggaat attaatggtg
     3001 gtgcccagag catttggtcc acctaactca tggatggcgc cggttcccgg atacagggta
     3061 cgtccatcct gatgcatgga tatatacagt acatccctgt catgccagta aatatcctga
     3121 gtgccgtcgc cgtgatgaca atcagtgtcc acaatagcaa ttttttttac aggatagtgt
     3181 tcacgaatat actccaccat cactgcttca atgttgatgt tacaaaaacc ccgtgctccg
     3241 tggacaactt tcatggcatg atgtcccggc gggcgcacca gtgcaaatga tttgtttaca
     3301 atgttatcca ttaccagcgt tgcagccttt atggtgccac ctgccgaaat taaatgcgat
     3361 tctgtggtca cctttgatac gtcaggaaag cagaaatggg tgcgcatgat atcattaaga
     3421 cttgcaatat caggtttgta ctgataaatg ccgtcaatat caaatacgcc ttcttcattc
     3481 aactggtcaa gggtgtataa taaccgttcc tcacgctcag ggtgcagcgg gtgtattgcc
     3541 cagtcaaagg ccggaaaaaa tacaataccc agtgttgatt ttgctttcat atcttgagta
     3601 tatccctgca ttgatgtaaa atgcccggtt taacctggca gtgaacacgg atgttcttgc
     3661 cggtagtttc aaacccttct accatgttga atgaatacac ctgcaaaatt tcaatttcac
     3721 gttcacgttc atcaacacct attgattcca gatactgcat catgtagtta gttgcatctt
     3781 ctatggcatt atcaacatca tactcttgcg gaatgcggat attaacatta aggtttggaa
     3841 caagtaacct gcagcgttga gtatcagcaa aaagctcaat atcaacggtt gtgcgtgcaa
     3901 cagctgcacc aatagcattg gccacttcgg cgtactgtgg caccttgacg ttccagccaa
     3961 accgttgcgt gatgtgatgt gcaagtgtct tggcaggacc gccaataaga tagatagttt
     4021 ttggtttcag cactttacct ttaaggaatt catgaatggt atagaccggc cgtgaattaa
     4081 cctcatcaat cattgccata acttcattgt atataatatc aagggcatag gtaattgcct
     4141 gttgtgcaat attatgtgat gatattcctg attttttttc aagagcgtac ataccctcac
     4201 gggagcgccc agtatccttt aaatttattg tgcctataac gttgagtgca tcaataattg
     4261 ttgggttttg tccaccttct gccattgaca tgcctaaacg ctgtggaccg gcagtaacag
     4321 tattaccgtc aatctttatt gctgaatcac caccaatacc aattgacttt gttttaagtg
     4381 aacgtaccag cgtattatgc gtgtttaatc gtatgccgtc tttttcaatt aatggagcac
     4441 catcggcaaa catggcaata tcacatgagg tgccgccaat atcaagcatc actgcatctt
     4501 cggtgatgtt gcacagtgcc atggtgccaa taacacttgc agcaggtccg gacaaaattg
     4561 attccacagg aatagtgcgg gatacatgca gcggaatggt accgccatcg gctttaagta
     4621 tattaacagg agctatcacc cccagttccc ttaagacttc ttcaatggcg tatgaaaatt
     4681 tattatacag gcgccacacg gcagcattgt agtatgctgt tgcaatacgt cgcgggaaat
     4741 taagattgcc agacaacgtg tgtcctacac tgataaaatc tgcgtcgtca agtttttgtt
     4801 gtatatactg ttcatgaaca ggattgcggg ttgaaaattt tgaaactact gcaaatgcct
     4861 tgatgccttt tttgcggcat ttttttgtgg ctttttcaag ctgattaaga ttaagcagag
     4921 tgatttcagt gcctctgtgg tcaatctgac cgtcgatgac atagtaatgg tttcccattg
     4981 aatagagagc aggattaata ccggggccgc ctgataccag tacgccaact tcttcacatt
     5041 tgttttcaat gattgcattg gtggtgagtg tagtgcttaa gttaactcgc ttaatatgcg
     5101 acgggtcaat acccgttaaa atatttttaa aggcctgact aatacattct agaaggttat
     5161 gtggtgtagt aacaacttta gtgtgagcca ccacacccga tttgtcaatg ataactgcgt
     5221 cagtatgtgt tccgcctaca tctaatccta agtacataat tatcaccagc tacctgaggc
     5281 tccaccgccg ccaaagctac cgccaccacc actgaaacca ccaccagagg aaaatccacc
     5341 gcttgaaaat cccccgcccg aagaaaatcc accaccaccg gtcggcatgg atgcaatgat
     5401 gattccacca ataaatccaa taaaagccag taaaacaagt gcaggtactc caaacccaaa
     5461 aaatgcacca attatgggaa gcacaatagc gcccagtatc ccgctcatta agcgccctaa
     5521 cgcactgata aacgtcatga ctataaaggc aatccacata aaagcaccat aatcaggagc
     5581 agatttttga ggctttaaac tgcttgcatc aaattttccc tttacaatat caatgatggc
     5641 atcaacggct tttgtaatgc ctgcgtcgta gtcgccattt ttaaattcgg ggatgatgat
     5701 ataatcaatg atgcggccag caagcagatc cgttaacgtt tcttctaagc ctttgccaac
     5761 ctcaatgcgc actttgcggt tatcgcgtgc aactaacaca atgacaccat tgtccagacc
     5821 cttttgtcca atcttccagg tatttgccaa acgtatggaa taagactcaa tatcctcatt
     5881 gccaattgaa ggaacagttg ccacaaccac ctgcgttgaa tcatcttttt ccagttgtgt
     5941 taattttgcc tctaattcag ttttggttgt gggtgaaagt atatctgcat agtcattgac
     6001 gtgtgcctgt aaaggcggta tgggagctaa ctcctgagcg tacgcagtgg cacctacaca
     6061 caccgcacac agtactgtga aaacccattt cattaattgt caccaacaat tatattttta
     6121 agctcatcaa catcatcagg tttatacgga tactcttttg caagcctgtt gccaatagtt
     6181 tcaatggttg cacacagcgc aggaataaat tgcttttgtt ttatgccggt aatgatgtgt
     6241 tttacgcatg tttcccagaa ttcaggttca atttttttat gggcatcttc atcggcaagt
     6301 atccatacct ttttttcaag aagcgttata tatacaagca cgccgttttt atcagctgtt
     6361 ttatacaggc catttttgta aaatgcggtt atggcagctt cacgcacttc tgcatcaact
     6421 tcgcgcgggt ttaagaaaag gcgtgttaaa aaaacgcttc tggcgatagt aaccttgaac
     6481 acaataaaca gcagactaaa aaaaacaata aatatccaca gattgtcttt gtgaaagtag
     6541 aaaagcggcg caatgtagta ggtcagtgca atcgccagag gtagcgacag tacagcagat
     6601 gcatatatgc gtgtaagcgg ataatgatag cttgcactga caatcatggg gacaatttcg
     6661 gctgatgtca gcttttcagc ttttttaacg gcatcaatga tgcactgggc atcagcctgc
     6721 gatagtattt tagaaacaag atctttcatt ggtaactatc gcctattcaa aagaaacttt
     6781 gggagcagtt ttagcgccct cttcagcctt gaagtattcc ttgcgctcta agtgaagcag
     6841 gaggctgttg gttatgttgt acggaaaaat tctgattgat gaattgaatt ccttaaccgc
     6901 ttcgttatac cgctggcgag ccacattgat gcggttttcg gttccttcaa gctgatgcat
     6961 caggtcagtg aagtgcgcgt cggcttttaa atttgggtag cgctcaatta ccaccatgag
     7021 tcgtgccagt gcgcttgaaa gctcgccctg cgctgcctga aattttgcaa tggcttcagg
     7081 gttgctcagg tttttgggcg atagttgaac ggaagtggcc tttgcccgcg cttcaatgac
     7141 agcctgtagt gtagtctttt cgtgcgcggc atatccctta acagtggcaa ctaagtttgg
     7201 gataaggtca gcccttcgtt gcagtgccga ttctaaatcg ccatacgcct tgaatacctc
     7261 ttcttcctgc atctgaagtt tgttatatcc gcatcccgta aaggaaaata gcgacaatgc
     7321 aattataact gctaaagaat aggaaatttt tttcataagc cctcctggtg gggaaagata
     7381 aaccgctgtt ttgcgtaaaa ttaaaatata cacttaaaac aaaaaggtgt caagaataaa
     7441 aaatatccca cagagagcac agaatgcagt ggaaaagagg ggtatctccg tacaaaaatt
     7501 atacttgcaa atataataat gtataattac gttatagaac aaatacataa tgcaaaatat
     7561 atttgaagat agattcagat taaaaatgta ggaatgagca aataatgaaa caaagtagtg
     7621 ttgaagaaag aaaaatatta cttgatgcgg aattaaagaa aatagtagaa gttcttaaac
     7681 aggaatatga tcctgaaaag ataatacttt ttggttcatt gttagatgga aacatccatg
     7741 aatatagtga tattgattta ttgataataa aaaaaacttc aaaacgccct atagacagga
     7801 tacttgaagt tgcacaaatt ataaatccat cattgggggt tgatttattt gtctatactc
     7861 cagaagaatt tgacacactt gtaaatgaaa aatatacttt ttttaccaat attttaaaaa
     7921 atagcaaaat tctttatgaa aaaagaaaca gcagagtgga ttagtatagc cagtgaggaa
     7981 ttaatggctg caaaacatct ggaaaaactg aatttgcaca ggatggtgtg ttatcattgt
     8041 cagcaatgtg ttgaaaaagc tatgaaagca ttcttagtgg aaaaagataa agaattccaa
     8101 aggacacata atattcttga tttgaaaaat atggtagtag catcaggtgt tgatattcca
     8161 ttaaccaatg atgaagctgt ttttttaaag aataatatac tataaaatcc ttgctctcat
     8221 cgtgatgaaa tgaagatgca tcacataggg agcgcaaaga aaaaaaataa aaaaatttta
     8281 aatttttcaa tataaaagcg tttatatata taaacacgat tatggaatgg atggaattaa
     8341 tacaggtgga gttatgcttt ctttgctgat gtgattgttg tatagtgata gaaagataat
     8401 gctacacctg ttatgcttga catttcataa taatcgtgat attatttgat ctggaaggag
     8461 ataaaaaaat gagcatgcat ctttcggctg atgtgtatga gatttttgaa aaggcttttc
     8521 agggcaaaaa tgaagcaaaa aaggtaatgt atgcaattga agaggcaatt gtgagtacag
     8581 tgcatgacac atggtacaaa acaaaggaag agttaaagct agaagtttta agcttctatg
     8641 ccactaagga tgatttactg caggtgcgta ctgaactgct tgccgaaatg aaaaaagaca
     8701 aggcagagat tttagggata atgaaacagg ataaggcgga gctatcaggt ataatagaac
     8761 agaacaaagc tgaactttta gggataatga agcaggataa agcagagcta tcaggaaagt
     8821 tggaagcatt gtatgaaaag acagaaagag acaaatctga acttttagga ataatgaagc
     8881 aggataaagc agagctttta gggataatga aacaggacaa ggctgagcta ctagggataa
     8941 tagaacagaa caaagctgag cttttaggta aatttgatgc actatttcag aagacagaga
     9001 aagacaaagc tgaattgtta gggataatga aacaggataa agctgaattt gctctaaagc
     9061 tagagaaatt ggataaaaaa ttcagtatgt attttgcggt gatgatgttt gccattatat
     9121 tcctgaatca aaatgcgctt gagtttcttg ctaagatttt aggattaata cgataaaata
     9181 aatagtacgc cgagagcgca aagtactcag tggaaaagag ggatcagaac ctcattaaaa
     9241 aaaccgtaca gccatactga aaaatttttg aaaatgtata aaaaagtgtt gccatataat
     9301 tgtttttttg cacttttatt agtatattgc tgtaattaat acatatataa tattcttact
     9361 attatatgtg taccagttct tttttataat tgagatgctt tgtaccatag ctcctgcctt
     9421 ttgtacatca taagctatag agaaaactgt tgttattttc gtgtaatggt ataacaaagt
     9481 gaacattcac ttttaaggtt taaaaaagca aagagcaaag gcactataca caatctaccc
     9541 tgcttggagg aaaaccatga aacgtcatat actaaccttt ttaataacac ttcttgttgc
     9601 ggcattgttt gcttgcagct atgaaaacac agctaccgta accattgata ctgggatacg
     9661 tcagcaggcg caattgagct tatttgatag ag
//
LOCUS       DAORYL010000100         9474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_5396_length_9474_cov_13.258945, whole genome shotgun sequence.
ACCESSION   DAORYL010000100 DAORYL010000000
VERSION     DAORYL010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9474
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5396_length_9474_cov_13.258945"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PLV81_13600"
     CDS             <1..709
                     /locus_tag="PLV81_13600"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HOM11174.1"
                     /translation="ELYQRQLIINGWGKEAQEKIKNTHLFIAGAGGLASPAALYCAVA
                     GFGKITICDFDTISLSNLNRQILHNHTRMGMHKADSAYATLTQANPYIQVEIKNTKIT
                     TRNIQALIENSHIILDCLDNFETRHILNKACLQLHKPLIHAGVEGFSGQLTVVIPHQT
                     PCLSCFLPKKSNKKPIPIAGVTPGVMGTLQAMEAIKLVTGLGAVTTGKLLLFDGLAMR
                     FSSISLEQNPACKVCGK"
     gene            complement(790..3390)
                     /locus_tag="PLV81_13605"
     CDS             complement(790..3390)
                     /locus_tag="PLV81_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HOM11175.1"
                     /translation="MENIVFFNKDFTGVDDKQYKKLGIRGRRAADLAKMGLPIAPGFI
                     IDADLTMKLPKVNVKQLIKTFVDKIEKDMKKGYGDSEKPLLLKVVLSSDLNVPFFPSI
                     HNVGLNDKVVTGFAKFTGEDFAWGEYRFLLRSIASKIYGIDEKELDELEGKLQKNAKA
                     KEYKEVVAKYKKFLGDKFSEDVYDQLGLILKGAAARYCDSDIDVDNSLSIMIQAMVYG
                     NFGQNSFAGNYFTRNIINGDALIQGEFLQNEFDVDRGTPKDIAKIDKKYYDKLVEIAK
                     KVEDNFKEIRNIKFTIEEGNFWLIEQREVDEKSTQAHIKTLLDLHSRKVVAPEFLIES
                     IKPSQLNELLHPVIDPRTTKNVKSIKGGIAGSTGAAIGRVFFSTDKLLEEYKRAVMHG
                     GDTNLILVMPATYAEDVKAIEVARGVITSEGGFSSHAPVVARSLGKVAMVQPEMKIRG
                     NSFTLAGKTVKEGDYVSINVPYYEPPTIYLDKVGLIEPDFEKNGLTDFLTIVQKFIKD
                     FDVRANADQPRDAKVAKMFFADGIGLCRTEHMFFNEKRIMKFREMILSETEAERRKAL
                     DDLKPMQRGDFYELFKIMQGHPVTIRLLDAPLHEFLPRTEDSMKEFIQYMKSRKKGMS
                     DAEIRARCEELSEMNPMLGHRGCRVAITYPEIYEMQCRAIFEAACMLKKEGINVVPEI
                     MIPIVMTETEIKFIKNGKKIEGKTVKGIRDIKDEVVEEYGIDDLEYEVGTMIELPAAA
                     VGAGRIAQYAEFFSFGTNDLTQTTYGLSRDDINSFFPSYSLYDLIPNNPFKVLGREVK
                     ELIEIAATRGKLTRPDITMGLCGEHGADPDNIEFCMNVGLNYVSCSPYAIPLSKLAIA
                     QLNIKQAKSK"
     gene            complement(3425..4330)
                     /locus_tag="PLV81_13610"
     CDS             complement(3425..4330)
                     /locus_tag="PLV81_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11176.1"
                     /translation="METTFYSLATKASFNKALNHFYSVNDVVDEVEGGRNFADVVNIM
                     LSEGDLMVDQVLPMVGAILRDKYQYTFDSYNVPVTMNDFSKIVEETSKWTALDIVIVY
                     FNVNGEPILINPKNTAHWERVREIVRDQLMVIYTKKKKEANPKVENEAINAIEEMIAG
                     KDVFINKEFIDETIAVKEVKKPTPAAVAAAGKKNMTPKYAVTVTNELFHNGNVEAWKR
                     IIESFKVSHPDLDVLIFHEGEKINDINSLFKWGKVKHGDPIFFQVVGENIRDVSKLQK
                     YLFEGASPRFEQFLKIAVGKVLNLF"
     gene            4566..4636
                     /locus_tag="PLV81_13615"
     tRNA            4566..4636
                     /locus_tag="PLV81_13615"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4598..4600,aa:Gly,seq:tcc)
     gene            complement(4647..6086)
                     /locus_tag="PLV81_13620"
     CDS             complement(4647..6086)
                     /locus_tag="PLV81_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HOM11177.1"
                     /translation="MKRILFILLFIISCSQKYTDYTPFTIQLIYDQSHNNVWGLWDHG
                     YCGYSHIAGVLKQHGIPASVNLKKLDECISSLPSMGYSNAILMLSVAKYQRFDDREID
                     AISNFVNAGGLLFVIAEHDNMYNSATFLNAVTHKMGITINSDAAGKDNDAFQSLDDLN
                     NRAYSKKFKLKNVLHMLSASLSGTADSFEVLLRDDETDNIIAGGCRYGKGKMLVVGDS
                     EMFWNGDGKVGIHAGDNSAFFKHCIEWLLEKHLAHTVQHAVTVPCFDAENSYIAIDKS
                     PSGVEHFINALTKINPSLLHDDSINIIVIPMPNCGINNTNRNIIFIEPYQKLIPSTVW
                     GKRLIALGAVNTKVYSFLGDSNCEILPCLITDGDTNFFDVVISCNKKRFYFHRLAGMI
                     KKGNGGVIAQIPSSLWGEVSHPGLEVLNDGIPLYQPNDYETAGFVYADKNLLVVGDAD
                     AIANQNRDTETFKEILAMVMRWIKTGNID"
     gene            complement(6132..7496)
                     /locus_tag="PLV81_13625"
     CDS             complement(6132..7496)
                     /locus_tag="PLV81_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOM11178.1"
                     /translation="MAKDSTKIQLPEKEMPRQWYNILADLPKSMDPPISPATGKPVTP
                     DELHAIFPMGLIEQEVSPKKYIDIPEDILHIYRLWRPSPLFRARRLEAALGTPANIYY
                     KYEGVSPAGSHKLNTAVAQAYYNKLEGIKKLATETGAGQWGSALSLAGNFFGIDVHVY
                     MVRVSYEQKPYRKFMMETWGATVTPSPSNLTNSGREILKQDPDSPGSLGIAISEAVEE
                     AANSKDTNYSLGSVLNHVCLHQTIIGLETLKQFEMVDDYPDVVIGCVGGGSNFAGLAF
                     PFVRDKMNGKNIDIIAVEPAACPTLTKGMYTYDYGDVAKLTPIMPMYTLGHSFIPPSI
                     HAGGLRYHGMSPLVSFLYHEGLISAMALHQKECFEAAILFSKTEGIIPAPESSHAIRA
                     AIIEAQKAKEEGKQKTIVFNLSGHGHFDLASYDKFLSGELDDYEYPKELIDKALHDLP
                     KVGK"
     gene            complement(7781..9157)
                     /locus_tag="PLV81_13630"
     CDS             complement(7781..9157)
                     /locus_tag="PLV81_13630"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOM11179.1"
                     /translation="MVGICGVGMSALAGMLLSLGHNVSGSDTDMYPPISTQLEMLNIA
                     LFKGYSSDTIEKIQPDLVIIGNAISRGNPEVEALLNKRIPYLSMAQSLYNFFLYNKEV
                     IGVAGTHGKTTTTSLLAYILDIAGMEPSFFIGGILKNYGKNYHVGRGKYFVIEADEYD
                     SAYFEKIPKFMVYRPTHCIMTSLEFDHADIYKNVDEIAVWFTHLKNLIPANGCIVYNR
                     MYPHLCAINEKPLSLTKSYGMSDADCTYHYKDGILTISMDGITIHAKPKIFGNFNYQN
                     IAAASAIALTLGVSEGAIVSAIESFEGVKRRQEILYASNYLTIIEDFAHHPTAIYNIL
                     HEVSEHYPQSKIIAIYEPRSATSRRNVFQQELPLAFSDADCVVMKRPYRLNKVPEQEQ
                     INIDRVIDDIKGQSITAYVFDSVDEIVHEAFKQIQNKHTVMVVMSNGGFDGIYDLLVS
                     QAQKLFGK"
BASE COUNT         2848 a         2068 c         1575 g         2983 t
ORIGIN      
        1 tgaactctat caacgtcaac tcattattaa cggctgggga aaagaagcac aggaaaaaat
       61 caagaatact caccttttta tagccggtgc cggggggctt gcaagccctg ctgcattata
      121 ctgcgccgtt gcgggttttg gcaaaattac aatctgcgat tttgatacca tttcactatc
      181 aaatcttaac cgacagatac tccataatca tacacgaatg ggcatgcaca aagctgactc
      241 agcgtatgca acattaacac aggcaaatcc ttatattcag gttgaaataa aaaatacaaa
      301 aattactaca cgaaatattc aggcattgat tgaaaactca cacataattc tggattgcct
      361 tgataatttt gaaacgcggc atatactcaa taaagcatgc ctccagttgc acaaaccact
      421 gatacatgca ggcgtagaag gtttcagcgg ccagcttact gtcgttattc ctcatcaaac
      481 gccatgccta tcatgttttc ttcctaaaaa aagcaataaa aagcccattc ctattgctgg
      541 cgttaccccc ggtgtcatgg gcaccctgca ggcaatggaa gcaataaagt tagttactgg
      601 gcttggcgct gtgactacag gaaaacttct cctttttgat ggacttgcca tgcggttttc
      661 atctatttca ctggaacaaa atcctgcatg caaagtttgc ggaaaataac aaggctgctt
      721 ttgtgtaagc agccttaatg gatttacaat catatgggcg atagttactg ataagacatc
      781 atcactgtat tatttactct ttgcctgttt tatattcaac tgagctatcg ccaacttaga
      841 tagtgggatt gcatatggtg agcatgatac ataattcaat cccacattca tacagaattc
      901 gatgttatca ggatcagcac catgttctcc acacagcccc atggttatat caggacgagt
      961 gagcttaccg cgtgttgcag ctatttctat aagttctttc acttctcttc ccagaacctt
     1021 aaatggatta ttgggaataa ggtcatacag tgaatagctt gggaagaatg aattaatgtc
     1081 atctcgcgat aatccatacg tagtctgtgt aaggtcattg gtaccaaaac taaagaactc
     1141 agcatactgt gcaattctgc cagcaccaac cgccgcagcg ggtaattcaa tcattgtacc
     1201 aacctcatac tctaaatcat caataccata ttcctcaacg acctcatcct tgatatcacg
     1261 aatacctttt accgttttcc cttcaatctt tttgccattc ttaataaact tgatttctgt
     1321 ctcggtcatg acgataggaa tcattatctc agggacaaca ttgatgcctt ctttcttcag
     1381 catacatgca gcttcaaaaa ttgccctgca ctgcatttcg taaatttcag gatatgtgat
     1441 agcaacacgg caaccccggt gtccaagcat tggattcatt tcagatagtt cttcacatcg
     1501 cgcacgtatt tcagcatctg acattccttt tttacgcgat ttcatgtact gtataaactc
     1561 tttcatactg tcttctgttc tgggcaggaa ttcatgcaat ggagcatcaa gcaaccgtat
     1621 ggttacagga tgaccctgca ttatcttaaa caattcatag aagtcacccc tctgcatggg
     1681 tttgaggtca tccagcgctt tccgccgttc agcttcagtt tctgataata tcatctctct
     1741 gaacttcata atgcgctttt cattaaagaa catatgttca gtgcggcata aacctatacc
     1801 atcagcaaag aacatctttg ccactttggc atcacgtggt tggtcagcat tggcacgcac
     1861 atcaaaatct ttaatgaatt tctgcactat tgttaaaaag tctgttaacc cattcttttc
     1921 aaaatctggt tctattaagc ctaccttatc cagataaatt gtaggaggct cataatacgg
     1981 cacatttatt gaaacataat caccttcttt tacagtttta cctgccagag taaacgagtt
     2041 acctctaatc ttcatttcag gctgaaccat agcaacttta cctaagctac gtgcaacaac
     2101 gggagcatgt gatgaaaaac caccttccga tgttattaca ccacgcgcaa cctcaattgc
     2161 cttaacgtcc tcagcatagg ttgcaggcat aaccagtata aggttagtgt caccaccatg
     2221 cattacggcg cgtttatatt cctcaagcag tttatccgtt gaaaagaata ccctaccaat
     2281 agctgcaccg gttgaacctg caatgccacc ttttatcgat ttcacatttt tagttgttcg
     2341 cgggtcaatg accgggtgaa gaagttcgtt aagctggctt ggttttatag attcgattaa
     2401 aaattcaggt gcgaccactt tgcgtgaatg caggtcaagt aatgtcttga tatgagcctg
     2461 tgttgacttt tcatcaactt cacgttgctc aatgagccag aaattacctt cttctatggt
     2521 gaatttaata ttacgtattt ctttaaagtt atcttccact ttctttgcta tctcaactaa
     2581 cttgtcataa tactttttat caatcttagc aatatccttt ggagtaccac ggtcaacatc
     2641 aaactcattc tgcaagaatt cgccctgaat gagtgcatca ccgttaatga tattgcgggt
     2701 aaaataattt cccgcaaagg aattttgtcc aaagttacca tacaccattg cctgaatcat
     2761 aattgacaat gaattgtcaa catcaatatc cgagtcacaa taccgtgctg cagcaccttt
     2821 aagaattaga cccaactggt catacacatc ctcactgaac ttgtcgccca gaaatttttt
     2881 atatttagct accacttctt tatattcttt agcttttgca tttttttgga gtttcccttc
     2941 cagttcatcc agttcttttt catcaatccc gtaaattttt gaagcaatgc tgcgaagtaa
     3001 aaatctgtat tcgccccagg caaaatcttc cccggtaaat ttggcaaaac ctgtaacgac
     3061 cttatcattt aaaccaacat tgtgaatgga tgggaaaaaa ggaacattta aatctgagct
     3121 taataccact ttcaaaagca atggcttttc actatcgcca tatccttttt tcatgtcttt
     3181 ttctatttta tcaacaaagg tcttaattaa ttgctttaca ttaacctttg gtaatttcat
     3241 ggttaaatca gcatctataa taaatcccgg agctatcggt aatcccattt ttgctaaatc
     3301 agcagctctg cggcctctga tacctaactt cttgtattgc ttatcatcaa caccggtaaa
     3361 atctttattg aaaaaaacta tattttccat actaccctct ctttttaatt tgtttcatat
     3421 ggtattagaa aagatttaat actttcccaa cagctatctt caagaactgt tcaaaccgtg
     3481 gcgatgcacc ttcaaacagg tacttctgta atttagacac atcccgaata ttttccccaa
     3541 ctacctggaa gaatatggga tcaccatgct ttactttccc ccacttaaac agtgagttaa
     3601 tatcattgat tttttcaccc tcatggaaga tcaacacatc caggtcagga tgtgaaactt
     3661 tgaagctttc aattatacgc ttccatgctt caacattacc attgtggaac agttcattgg
     3721 taacagtaac agcgtatttt ggcgtcatat tctttttgcc ggcagctgcc acggcagctg
     3781 gagttggctt tttcacttct ttaacagcaa tcgtttcatc gataaattct ttattgataa
     3841 acacatcctt tccagcaatc atttcttcaa tagcatttat tgcttcattt tcaacctttg
     3901 ggtttgcttc ttttttcttc tttgtataaa tgaccataag ctgatcgcgg acaatttcac
     3961 gaaccctttc ccagtgggct gtatttttag gattaataag gattggctca ccattgacgt
     4021 tgaaatatac aatgacaata tcaagggctg tccatttaga tgtttcttca actattttgc
     4081 tgaaatcatt catggttacc ggcacattat atgaatcaaa cgtatactgg tatttatcac
     4141 gcaaaatagc cccaaccatg ggcaatacct gatccaccat caaatcccct tcagaaagca
     4201 tgatgtttac cacatcagca aaatttctac ccccctcaac ctcatcaaca acgtcattaa
     4261 cactatagaa gtgatttaaa gccttgttaa aactagcctt agtagctaat gaatagaatg
     4321 ttgtttccat ggatttccct ctttttgaaa atttattatg aatgcacaaa acgcaatagc
     4381 tatatataaa ataatcaaaa ataaaatatt tttttgattt tttactataa tggtattata
     4441 aaattataaa gattgtttta caagtatttt tttattttta tttggcgata gttaccgcca
     4501 tcgttgcctg aaagatttat ttattgacaa aaagtggctt ttgctaaaat ctgtcgtcaa
     4561 tatacgcggg tgtagttcaa tggtagaact tcagccttcc aagctgatag cgtgggttcg
     4621 attcccatca cccgctcatt aagatattag tcaatatttc ctgtttttat ccagcgcata
     4681 accatggcaa gtatctcttt gaatgtttcg gtatcgcggt tttgattggc aatagcatca
     4741 gcgtctccaa ccactaaaag atttttatct gcatacacaa aaccagctgt ttcataatcg
     4801 tttggctgat ataatggaat gccgtcatta agaacttcca gaccaggatg gcttacctca
     4861 ccccacagtg atgacggtat ctgcgcaatg acaccaccat tacctttttt tatcattcct
     4921 gcaagcctgt gaaaataaaa cctcttttta ttacaggaaa tcacaacatc aaaaaagtta
     4981 gtatcgccat cagttataag gcagggaagt atttcacagt tgctatcgcc caaaaacgaa
     5041 taaaccttag tatttacagc accaagcgca ataaggcgct tgccccatac cgttgatggt
     5101 ataagttttt gatatggttc aataaaaatg atatttcgat ttgtgttgtt aataccacaa
     5161 ttgggcatgg gaatgactat tatattaatt gaatcatcat gcagtagtga ggggtttatt
     5221 tttgtgagtg cattaataaa atgctctaca cccgatggcg atttgtcaat agcaatatat
     5281 gaattttcag catcaaaaca tggtacagtt acagcatgtt gtacggtgtg agccaaatgc
     5341 ttttccagaa gccactcaat gcagtgttta aaaaaagcac tgttatcgcc agcatgtatt
     5401 ccaaccttgc catcgccatt ccagaacatc tcgctatcac ccaccacaag cattttgcct
     5461 ttgccatacc tgcatccacc agcaataatg ttatctgttt catcatcacg caataacacc
     5521 tcaaaagaat cagcagttcc cgatagcgaa gctgaaagca tgtgcaaaac attttttagt
     5581 ttaaattttt ttgaatatgc cctgttattc aaatcatcaa gcgattggaa agcatcattg
     5641 tctttcccgg cagcatcgct gtttattgtg atacccattt tatgcgttac tgcatttaaa
     5701 aacgttgcac tattgtacat attatcatgt tcagcaatga caaaaagaag acccccagca
     5761 ttaacaaaat tgctgatggc atcaatttca cgatcatcaa accgttgata tttagcaacc
     5821 gacagcatca gtattgcatt gctgtatccc atggaaggca acgaagatat acactcatca
     5881 agttttttca aattaactga agcagggata ccatgctgtt ttagcacacc agcaatatgc
     5941 gaatacccgc aatacccatg atcccacaat ccccacacat tattgtgtga ttggtcatag
     6001 attagctgta tagtaaatgg tgtgtaatcc gtatattttt gggaacagct gataataaaa
     6061 agcaggatga aaagtattct tttcatattc cataatggtg gggcgcacgg taatgcgccc
     6121 ctctaagaac attatttacc aacttttggc aaatcgtgca aagccttatc aattaattct
     6181 tttggatatt cgtaatcatc gagctcaccc gataaaaact tgtcatacga tgccagatca
     6241 aaatgaccgt gaccactcaa attaaatact atagtctttt gcttgccttc ttcctttgct
     6301 ttctgtgctt caatgatagc agctcttata gcatgagatg attcaggcgc agggataata
     6361 ccttcagtct ttgagaacaa aattgccgct tcaaagcatt ccttttgatg caatgccatt
     6421 gccgatataa gtccttcatg atacaagaaa ctcaccagtg gtgacatacc atggtaacgc
     6481 aatccgccag catgtattga aggagggata aatgagtgac ctaatgtata cattggcata
     6541 attggcgtaa gttttgcaac atcaccataa tcatacgtat acatgccttt tgttaatgta
     6601 ggacatgctg caggttctac tgcaatgata tcgatatttt taccgttcat tttatccctt
     6661 acaaatggaa atgcaagccc agcaaagttt gaaccgccac caacacaacc aataacaaca
     6721 tcaggataat catcaaccat ctcaaactgt tttaatgttt caaggccaat aattgtctga
     6781 tgcaggcaca catgatttaa cacactgcct aaagaataat tggtatcttt gctgtttgca
     6841 gcttcttcca ctgcctcact gattgcaatt cccaaactgc caggactatc aggatcctgt
     6901 tttaatattt cccgcccgct gttagtaaga ttgcttggac ttggtgtaac cgttgctccc
     6961 cacgtttcca tcataaactt tcgataaggc ttttgttcat agcttacccg taccatatac
     7021 acatgcacat caatgccaaa gaaattgcca gcaagtgaca gagcactacc ccactgtccc
     7081 gctccagttt cagtggctaa tttcttaatg ccttcaagtt tattataata cgcctgagca
     7141 actgctgtat taagcttatg gcttcccgca ggcgaaacac cttcatattt ataataaata
     7201 ttggcagggg ttcctaaagc tgcttctaac cgacgagccc tgaaaagcgg tgaaggtctc
     7261 cacaaacgat aaatatgcaa tatgtcttcg gggatatcaa tatatttttt gggactaact
     7321 tcctgttcta ttaatcccat ggggaaaata gcatgcaatt catcaggtgt taccggttta
     7381 ccggttgcag ggcttatagg cgggtccata gacttgggta aatcagccag aatattatac
     7441 cattgccgtg gcatctcctt ttcgggtaac tgtattttag ttgaatcctt tgccattgta
     7501 tcctcctatt tgaatcattt aatttttaaa aaatattgcg tactacaaat aaaaaggcta
     7561 cggttaacac atattatccg tagcctttaa ggatttccct attataaatt aattacctca
     7621 agcgcccgga tatatgttat cctggcgccg ccaccaccaa actaaaatag ctattatatt
     7681 aagtttaccc actaccatat aaataacgtt taattttctt acatctttaa ccatgcataa
     7741 atcatacaac aaatgtcaac aattttttta tttacctttg ttactttcca aaaagctttt
     7801 gtgcctgaga aaccagaaga tcatatatgc catcaaatcc cccattactc ataaccacca
     7861 taactgtatg cttattctga atctgtttga atgcttcatg aactatctca tcaacagaat
     7921 caaatacata agctgtaatg ctctgaccct ttatgtcatc aatgaccctg tcaatattaa
     7981 tctgctcttg ctctggcacc ttattcagac ggtatggccg tttcatcacg acacaatctg
     8041 catcagaaaa tgccagcggt agttcctgct gaaatacatt gcgtctgctt gtggcagatc
     8101 gtggttcata aattgcaata atttttgatt ggggatagtg ctctgacacc tcgtgtaaaa
     8161 tgttgtatat tgctgttggg tggtgtgcaa aatcctcaat aattgtgaga tagttactgg
     8221 catagaggat ctcctgcctg cgttttactc cttcaaatga ttcaatagca gaaactatcg
     8281 caccctcaga taccccaagg gtcagagcta ttgctgacgc tgcagcaata ttctgatagt
     8341 taaagttgcc aaaaatcttt ggctttgcat gtattgttat tccatccata gaaattgtga
     8401 gaatgccatc cttataatga taagtacagt cagcatctga cattccgtaa cttttagtaa
     8461 gcgatagtgg cttttcatta attgcgcaca aatgcggata catacgatta tacacaatgc
     8521 atccattggc aggaataagg tttttaaggt gagtaaacca tactgcaatc tcatcaacat
     8581 ttttatatat atctgcatga tcaaattcaa gtgacgtcat gatacagtgt gttggccgat
     8641 acaccataaa tttgggaatc ttttcaaaat aagctgaatc gtattcatcc gcttctatga
     8701 caaaatattt acctctccca acatgataat tttttccata atttttgaga atgccgccaa
     8761 taaaaaatga tggctccata ccagcaatat ccagaatata tgccaaaagc gaagttgttg
     8821 ttgtttttcc atgggtacca gctacaccaa tgacttcttt attataaaga aagaaattgt
     8881 acaggctctg agccattgaa agatatggaa ttcgtttgtt aagcaaagct tcaacttcag
     8941 gattaccacg gctgatagca ttgcctataa taacaagatc aggctgtatt ttttctatgg
     9001 tgtcagagct gtacccttta aaaagagcta tatttaacat ttcaagctga gtgctgatag
     9061 gtggatacat atcggtgtca gagcctgaaa cgttatgacc cagggaaagc agcatgccag
     9121 caagagcact cattcccacc ccacagatgc ctaccatatg aatatttaaa accgattttt
     9181 taaatatatc taacatcgcc agtacaccca atataagttt gctcttgttt catacaaaaa
     9241 ataatcaata tgattcccta caataattta caaaagtaat ttttctgtaa attgaaaaat
     9301 aaatttttaa acactaaatc aataattcac cctttacgta tgcgtaaaat ctctcaatac
     9361 actcttccaa tgtgttacct aactctaaaa aatattgatg gaaggtaatt tttatagtag
     9421 ggcgatagtt cctgtcaaag taagcccttg acccaccaaa atagctttca aaag
//
LOCUS       DAORYL010000101         8976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_5719_length_8976_cov_14.398274, whole genome shotgun sequence.
ACCESSION   DAORYL010000101 DAORYL010000000
VERSION     DAORYL010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8976
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5719_length_8976_cov_14.398274"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..929
                     /locus_tag="PLV81_13635"
     CDS             108..929
                     /locus_tag="PLV81_13635"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOM11180.1"
                     /translation="MSKRPGWWLYVLAKIWPITWISAQATQSKIIGPLIAKTLVPFFS
                     KKNLNITYLPINITIAPQSTPVPLRIVEEIIRRSAHRVIIKRCTCRDAKQCTEHPIDY
                     ACLLMGQGAMEIDSRIATHVGVDEAIAHLHKCVGEGLIPMIGRVKIDNLIWGVKDTGK
                     LLTMCFCCRCCCTILNSGKYLPEEAASSIVRLKGVTLSVNLDACTGCGTCVDECFMDA
                     IRIVDGKAVHNDTICKGCGRCAQVCPHGATTITIDNIDAAIDEINARIRQYVQYE"
     gene            957..1223
                     /locus_tag="PLV81_13640"
     CDS             957..1223
                     /locus_tag="PLV81_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11181.1"
                     /translation="MTSHKYFWYFLMIGALVLWACAIALIFLFPESDYRAVLLIALLI
                     VHCGEIPYTLKLLKSKVSPVTIATKTFLFGFTWWLPFNKGILKG"
     gene            1228..2052
                     /locus_tag="PLV81_13645"
     CDS             1228..2052
                     /locus_tag="PLV81_13645"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOM11182.1"
                     /translation="MFRYGPPKLFVKIAVALSWPIMILGKRLSAVPIVKHIINPFFKY
                     PYNEVTAVPINVAIAPAKSVTLPVQVLSEVVARVNHIFILDECICRSLLNCSNHPRTI
                     GCMALGEASHRIHPSHGHRSTAKEALDHIAKAAKAGLVASVAHVWIDPVAFWSVPFNK
                     LMFICFCDDCCCLYRTHMKKRGENLNKAYKKLPGVTVRVNFDACNGCGVCAQRCFVAA
                     IQMKDDMAVIGEDCKGCGRCVQVCPQQAVEIYFDDKDTLVANLVERINKVADIGLG"
     gene            2211..2597
                     /locus_tag="PLV81_13650"
     CDS             2211..2597
                     /locus_tag="PLV81_13650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOM11183.1"
                     /translation="MKVEQRINYGYVIYDITGDITYDNFTEIEDAIKDTLPDNFINVV
                     LNIERVPYINSSALGWLVKLMKEVNAKGYHFFLMNVNQEIMGLLKLTNTLQYFKIIPN
                     EQVLVEKEKKKELDKILNEIEEEKQE"
     gene            complement(2602..3558)
                     /locus_tag="PLV81_13655"
     CDS             complement(2602..3558)
                     /locus_tag="PLV81_13655"
                     /inference="COORDINATES: protein motif:HMM:NF013317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HOM11184.1"
                     /translation="MLQVHATTFAAARQALALALVKQKECSISIDPLVQHHIHYNRTL
                     QAIVEVLHQLNISCTYHNGIITVLPQKLPACTATITLHSFCPVTDIFLTIAPALSCNS
                     IQSDITFTGVTHCQYTHSTGFIRFGLAPVLSQFGCFLHMATKKFGFYTATGNAVAKIY
                     PQIKKEIGAIVTNTRITGVRIYIANIDQEFAFYQKKELCKALAVSDDSVSIMQVANVT
                     NHGNAIDVFFSTGDIPSMLSFTMPLYDDNGSFVFEDTMMHEFLHAVINTCLHSYEYIP
                     LPIVEEALPFLLAAGYDATVAQKIQPKIVGSEVYTMCKQFRD"
     gene            complement(3559..>4410)
                     /gene="serS"
                     /locus_tag="PLV81_13660"
     CDS             complement(3559..>4410)
                     /gene="serS"
                     /locus_tag="PLV81_13660"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOM11185.1"
                     /translation="PHYDLGTELNILDFERGVKIAGARFYVYRGLAAQLERAIINFML
                     DLHTKKHGYTEVFGPFIVNDESMIGTGQYPKFMDEYYRIERDALSLIPTAEVTLTNLY
                     RDEILDGKDLPIYVTMQSACFRREAGAAGKDTRGLIRVHQFQKVELVKFVEPETSFDE
                     LEKLVADAEEVLQHLNLHYRVVLLCSADTSAASSKTYDIEVWMPGLNRYVEISSCSNF
                     VDYQARRARIRYRKKEGEKPVFVHTLNGSGLAAGRTLAAVMENYQTKDGTITIPEVLK
                     PYLEKTL"
     assembly_gap    4411..4510
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4511..4692)
                     /locus_tag="PLV81_13665"
     CDS             complement(<4511..4692)
                     /locus_tag="PLV81_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOM11186.1"
                     /translation="MIDPKIVRENIDLVKHTLKIRKMEDAVDIAKLEELDSKRRSIIA
                     KIDELRTQRNSLSKQVG"
     gene            complement(4949..6238)
                     /locus_tag="PLV81_13670"
     CDS             complement(4949..6238)
                     /locus_tag="PLV81_13670"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOM11187.1"
                     /translation="MRKLLVFIVTFTLITCSNTQNSDFSLKYQDLQGNDEKCMVIYHA
                     NGAYGTPPVDTHVYNVNDSVTIIENQNLYKEGYYFIGWSMCPQCSPPIYKPGHTFTIT
                     ASTILYACFTTPAYTVIYLPGDADGGTTPIDTNYYCTGESAYILTNTGNLCKQGYRFD
                     SWFNANTQSYYQPGQSITIQNSNVELIAHFTPKPFTVTYNANGATTGTVPIDTMFYEQ
                     GELVTIAHNTGNLAIINREGVSYCFDYWYDSSDNVYLPGNTYPIEESLVLYAHFRPFA
                     VGDSGPCGGYVFYDKGYYSDGWRYLEAMRYDINASGIVWEQQLQAGQYRQTGATASDI
                     GTGYVNTCTIIQVLGEGDYAAYACAKYSDGWHLPSKDELQLLYHCKSIIGNFTSYNYW
                     SSTESTTSKAWCQNFNTGTQSTQYKNNTYRVRAIRQF"
     gene            6463..6933
                     /locus_tag="PLV81_13675"
     CDS             6463..6933
                     /locus_tag="PLV81_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11188.1"
                     /translation="MYIETTTCISHEHLEILKTQAKKHSMSLRTFLSALIGFAAQCDK
                     ARIKQFKQLKYRPRNNGAWKRFHLVLYGDEYEFFMDVKKLWKMSLALVIAYCLDNVLF
                     EFLKFLEEAEKDEDYYTDNYRFSGYTFEVSTEEDIFYCKFYWGPHPELVRKAFA"
     gene            complement(6945..7017)
                     /locus_tag="PLV81_13680"
     tRNA            complement(6945..7017)
                     /locus_tag="PLV81_13680"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6982..6984),aa:Phe,seq:gaa)
     gene            7056..7982
                     /locus_tag="PLV81_13685"
     CDS             7056..7982
                     /locus_tag="PLV81_13685"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11189.1"
                     /translation="MSIQKFLSIGFFIVTIAYAYVPSYAGSDASYYYKRALIQYKYKM
                     YNYAIESLELTLSSDPRHFEAANLLAKIYLDHYNDRVRALQYFEESLKINDNQPDIHL
                     EVGKLYYFFSEYSKAIEHLQRTIAQKNDVYAHYYLVLIYNLQKNYESAASHIAVCNAI
                     TLQQIKPEIEKAQAAQKDNNTNVAMAHYKKILEINPVNREAYVQLSHYYRIQKKIDMA
                     IQILEDCKKVYPKDTDVLLTLAHMYFEFKHPKRREYFIKQAIDLCKVAIGIDSSHCEA
                     YSLLFEIYKELGEVPLRDEHAAKYDACLGGSR"
     gene            complement(7986..8846)
                     /gene="purU"
                     /locus_tag="PLV81_13690"
     CDS             complement(7986..8846)
                     /gene="purU"
                     /locus_tag="PLV81_13690"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HOM11190.1"
                     /translation="MHSKESAILLISCPDKKGIVYKISHFIYSNNGNILRSDQHADEE
                     SGTFFMRIEWDLDGFAIEKNKIIKAFEPIAAEYSMNWQLHFSAKQQRTSIFVSKMDHC
                     LYDILLKAKEGEIATDIACIISNHESLGTIAKYFNIPFFVFQKTKENKLQQEQKELAV
                     LKDFDVDCIVLARYMQILSAHFVSHYPNKIINIHHSFLPAFKGAKPYHQAYQHGVKII
                     GATSHYVTELLDQGPIIEQDVIRVSHKDSIEDIIRKGKDIEKLVLSRALKKHLEHKVL
                     VYANKTIVFD"
BASE COUNT         2671 a         1622 c         1826 g         2757 t
ORIGIN      
        1 aggggtcaga gctatttcaa aagaactctt gctaaacaat ttagtagtag acttttttag
       61 aggatcaggt tatcatataa ataattttta gtataaagag gtgttacatg tcaaaacgac
      121 ccggatggtg gctgtatgtt cttgcaaaga tatggccaat aacctggata agtgctcaag
      181 ccacccagag taaaattatt ggccctttga ttgcaaaaac gctggtgcct tttttcagta
      241 aaaaaaatct aaatatcacc tatttaccca taaacataac tatcgccccg caaagtactc
      301 ctgtgccgtt acgtattgtt gaagaaatca taagacgctc agcacataga gtaattatta
      361 aacgctgcac atgccgagat gcaaaacagt gtacagagca ccctattgac tacgcctgtt
      421 tacttatggg gcagggtgca atggaaattg attcgcgtat tgcaacccac gttggagttg
      481 atgaagcgat agctcatttg cataagtgtg ttggcgaagg gctcatcccc atgataggaa
      541 gggtaaagat tgacaatctg atatggggag taaaggatac tggcaagctt ttaaccatgt
      601 gcttttgctg cagatgttgt tgcaccatac ttaattcagg taaatatttg ccagaagagg
      661 ctgctagttc aattgtgcgt cttaaagggg ttacgctatc ggtaaatctg gatgcatgca
      721 ccgggtgtgg cacctgtgtt gatgaatgct ttatggatgc aatacgtatt gttgacggta
      781 aagcggtgca taacgatact atatgcaaag gttgtggcag gtgtgctcag gtgtgccctc
      841 acggtgcaac tacaataact atcgataaca ttgatgcagc aatagacgaa ataaatgcgc
      901 gcatcaggca gtatgtgcag tatgagtaac ttttattatt ttcattggag gaacgcatga
      961 catcacacaa gtatttctgg tattttttga tgattggtgc gcttgttctc tgggcatgtg
     1021 ctattgcact aatttttttg tttccagagt cagactacag ggcagtgtta cttattgcac
     1081 tgttgatagt gcactgtgga gaaattccat acacgcttaa gctccttaaa agcaaagtgt
     1141 cgccggtaac tatcgctacc aaaacatttt tgtttggctt tacgtggtgg ctgccgttta
     1201 acaaaggtat actcaaagga taatcacatg ttcagatacg gtccaccaaa attatttgta
     1261 aaaattgctg tagccctttc atggccaatt atgattttag gtaagcgact gagtgcggtt
     1321 cccattgtca aacacatcat aaaccctttt tttaaatatc catacaatga agtaacagca
     1381 gttcccatta atgtggcgat agctcctgct aaaagtgtca ccctcccggt gcaggtgtta
     1441 agcgaggtgg ttgctcgtgt aaatcatatc tttatattag atgaatgtat ctgcagaagt
     1501 cttttgaact gctccaacca tccgcgtacc attgggtgca tggcactggg tgaggcaagc
     1561 cacaggattc atccgtcaca tgggcacagg agtactgcaa aggaggcttt ggatcatatt
     1621 gcaaaagcag caaaggctgg ccttgtggca agtgtagcgc atgtctggat tgacccggtt
     1681 gcattctggt cggtgccgtt taacaagctt atgtttatat gtttttgcga tgattgctgc
     1741 tgcctgtacc gcacgcacat gaagaaacgt ggtgaaaacc tcaataaggc atataaaaaa
     1801 ttgccgggtg ttacagttcg tgttaatttt gatgcatgca atggatgtgg tgtctgtgca
     1861 cagcgctgtt ttgttgctgc catacagatg aaagatgaca tggctgtgat aggtgaagat
     1921 tgcaaaggct gtgggcgttg tgtacaggtg tgtccgcaac aggcagtgga aatatatttt
     1981 gatgataagg atacactggt tgccaattta gttgaaagaa taaataaggt ggctgatatt
     2041 ggtttgggat agcaactcaa gtaatacatg cagggcaaat ggagtaaaat agttttacgc
     2101 agagtaaaca caggttgtta agctataaaa tctttgtaca cagtacattt tagcgttctc
     2161 agcacttgag gcgtgaaata atagataaat tattttgggg tcagagcatt atgaaggtag
     2221 aacaacgcat aaattacggt tatgtgattt atgacataac tggagacatc acctacgata
     2281 attttacaga aattgaagat gcaataaaag atactttacc ggataatttt attaatgttg
     2341 tgctgaatat tgaacgggtt ccctatatta acagctctgc actgggatgg cttgttaaac
     2401 tcatgaaaga agttaatgca aaagggtatc actttttcct gatgaatgta aatcaggaga
     2461 tcatggggct tcttaagcta accaatacat tgcagtattt caagattatc cccaatgagc
     2521 aggtgcttgt ggaaaaagaa aagaagaaag agttggataa gattttgaat gaaatagagg
     2581 aagaaaaaca ggaatagggg attaatctct aaactgttta cacatcgtat acacttcact
     2641 tcccacaatc tttggttgta ttttttgtgc gacagtagca tcataacctg ctgcaagtaa
     2701 aaacggcagt gcttcttcca caattggcag cggtatgtac tcataactgt gcaaacatgt
     2761 gtttataacc gcatgtaaaa attcatgcat cattgtgtct tcaaatacaa aactaccatt
     2821 atcatcatac agcggcatgg taaatgacag cattgaaggt atatctccag tgctaaaaaa
     2881 cacatcgatt gcattgccgt gattggtgac atttgctacc tgcatgatac tcacactgtc
     2941 atcacttact gccagtgctt tgcaaagttc ctttttttga taaaaggcaa attcctggtc
     3001 aatgttggca atatatatac gtacgccggt gattcttgta ttggtaacta tcgcccctat
     3061 ttcttttttt atctgtggat atatttttgc tactgcatta ccagttgctg tataaaaacc
     3121 aaattttttt gtggccatgt gcaaaaagca cccaaactgt gacagcacag gggcaaggcc
     3181 aaagcggata aaacctgttg aatgggtgta ctggcaatgg gtgactcctg tgaacgttat
     3241 atctgactgt atgctgttac atgataatgc gggtgcgata gttaaaaaga tatcagtcac
     3301 agggcaaaat gaatgcagtg tgatagtagc tgtgcatgca ggcagctttt gtggcaaaac
     3361 agtgatgata ccgttatgat atgtgcagga aatgttcagt tgatgtaata cctcaactat
     3421 tgcctgcagc gtgcggttat agtggatgtg atgttgcacc aaagggtcaa tactaataga
     3481 acattctttt tgctttacca gcgctaaagc gagtgcctgt cgcgctgctg caaaggttgt
     3541 tgcgtgtacc tgcaacattt atagtgtttt ttctaaatat ggttttaaga cttcggggat
     3601 ggttatggtg ccatccttag tttgataatt ttccattacg gcagcaaggg tacgccctgc
     3661 agcaagccct gagccattga gcgtatgcac aaacactggc ttttcacctt cttttttgcg
     3721 atatcgtatg cgtgcgcggc gtgcctgata gtcaacaaaa ttggaacatg aagatatctc
     3781 cacatatcgg tttaatccag gcatccacac ttcaatatca tacgtttttg aagatgctgc
     3841 tgaagtatca gcgctacata gcaacacaac ccggtaatgc aggtttaaat gctgcaacac
     3901 ttcttcagca tcagccacaa gtttttcaag ttcatcaaat gaggtctcag gctcaacaaa
     3961 tttaacaagt tcaaccttct ggaactgatg cacacgtata agaccgcggg tatccttacc
     4021 agcagcacct gcctcacgcc tgaaacaggc tgattgcatg gtgacataga tgggtaagtc
     4081 tttgccgtct aaaatttcat cgcggtacag gttggtaaga gtaacttccg cagtgggtat
     4141 taaggataat gcatcacgct caatacggta gtactcatcc atgaattttg ggtactggcc
     4201 ggtgccaatc atgctctcat cattcacaat aaatgggcca aatacttcag tatagccgtg
     4261 cttcttggta tgcaaatcca gcataaagtt tatgatggcg cgttctaact gtgcagccaa
     4321 accgcgatac acataaaagc gtgctcctgc aattttaacg cctcgctcaa aatcaaggat
     4381 attaagctcc gtgcccaggt catagtgggg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn cctacctgtt ttgaaagtga attacgctgt gttctaagtt catcaatctt
     4561 agcaataatg gaccgccttt tactatccag ttcttccagc tttgcaatat caacagcatc
     4621 ttccatctta cggattttta atgtgtgctt aacaaggtca atattttcgc gcacaatctt
     4681 tggatctatc atggtaacct ctttttacac ctatgaaatt aattgggctc atctaataag
     4741 tgttttcgct aaaaatttct ttatgcccat tagatattat tgccacacaa tggttcaaaa
     4801 tactatcgaa atatattcat caagtagtat accattggta aaattcaagt aacactcgtt
     4861 tattaaatat gcccaactat tttttaatgt ttaaaaataa atagtggaat ctcaaatatt
     4921 ttttgtcaag aaacaaaggg taacaatttc aaaattgtcg gattgcacgc actctatagg
     4981 tattattttt atactgtgtg ctttgtgtcc cagtgttaaa gttttgacac caggctttgc
     5041 ttgttgtaga ctctgtggaa ctccagtagt tgtaactggt aaaattgcct attattgatt
     5101 tacagtgata cagtagctgc aattcgtctt ttgatggtaa gtgccagcca tcagaatatt
     5161 tagcacatgc ataagctgca taatcacctt ctcccagcac ctgaatgata gtacaggtat
     5221 ttacatatcc cgtgccaata tccgaagctg ttgctcccgt ttgtcggtat tgtcctgcct
     5281 gaagctgctg ttcccataca attcctgatg cattgatatc atagcgcatg gcttctaaat
     5341 aacgccaacc atcgctgtaa tatcctttat cgtaaaacac atatccgcca cagggaccac
     5401 tatccccaac agcaaaaggt ctgaagtgcg catataatac caaagactct tcaatgggat
     5461 atgtattgcc aggaaggtac acattgtcgg aactatcgta ccaataatca aaacagtatg
     5521 atactccttc cctattgatg attgctaaat taccagtgtt atgggcgata gttaccagct
     5581 ctccttgctc gtaaaacatt gtatcaatgg gcacagtccc tgtggtagct ccgtttgcat
     5641 tataggttac ggtaaatggt tttggagtga agtgagcgat aagctctaca ttgctattct
     5701 ggattgtgat tgactggcct ggttgataat agctttgagt gttggcatta aaccaactat
     5761 cgaaccgata tccctgtttg caaagattcc ctgtatttgt cagtatatat gcactttcgc
     5821 cagtacagta atagttggtg tcaatgggtg ttgttccacc atccgcatcg ccaggtaaat
     5881 agatgactgt gtaagctggt gtagtaaaac atgcgtaaag tatggttgat gctgtaatgg
     5941 taaatgtatg tcctggttta taaatgggtg gcgagcactg ggggcacatg ctccagccaa
     6001 taaagtaata tccttcctta tatagatttt gattttctat aatagtaaca ctgtcattga
     6061 cattatatac atgagtatct acaggtggtg taccataagc accgttagca tggtaaatca
     6121 ccatgcattt ttcatcattt ccctgcaaat cctgatattt tagcgagaaa tcactgttct
     6181 gggtattgct gcatgtaata agtgtaaagg ttactatgaa tactagtagc ttcctcatag
     6241 atagctcctt gcagtaagat atgtgtagct atctattaat acgcttatgt agataaaaat
     6301 taaaaaggac tggaaaattt ttagtacggt gggtattcac tatggggtca gagcctcaac
     6361 gtaggagtaa ggggttttag gaaagatata gcaaaatagt tattgtcaaa tatttagctt
     6421 tagtgtatag tgagagtaca ataaatactt tgggggaatg taatgtatat agaaacaact
     6481 acctgtatct cacatgaaca tcttgaaata ctgaaaactc aggctaaaaa gcattcaatg
     6541 tcattacgaa catttttatc tgctcttatt ggatttgctg cacaatgcga caaggcacgt
     6601 ataaagcaat tcaagcagct aaagtataga ccacggaata atggagcatg gaagcgattt
     6661 catttagtat tgtatgggga tgaatatgag ttttttatgg atgttaaaaa gttgtggaag
     6721 atgtcgcttg ctttggttat tgcttattgt ttagataatg ttttatttga atttttaaaa
     6781 tttttagaag aagctgagaa agatgaagac tattataccg ataactatcg gtttagtgga
     6841 tatacttttg aagtttctac agaagaagat attttttatt gcaaatttta ctggggacca
     6901 cacccggaat tagtacggaa agcctttgct tgaaataaaa aaattggtca gggacggaat
     6961 cgaaccgccg acacggggat tttcagtccc ctgctctacc gactgagcta cctgaccaat
     7021 tgttatgcgt gtacacttaa tgaacggctt taaaaatgtc aatacaaaaa tttttgtcaa
     7081 taggtttttt catagtaact atcgcctatg catatgtacc ttcatatgct ggaagtgatg
     7141 cttcatatta ctataaacgc gctcttatcc agtataaata caagatgtat aattatgcca
     7201 tagaaagttt agaattaaca ttaagctctg accccaggca ttttgaagca gcaaacttac
     7261 tggcaaaaat ttatctggat cactacaacg atagggtcag agccttacaa tattttgaag
     7321 aatctctaaa aatcaatgac aaccagcctg acattcattt agaagttgga aaattatatt
     7381 attttttcag tgaatattca aaagcaattg agcatttaca aagaactatc gcccaaaaaa
     7441 atgatgtgta tgcccattat tatttagttc ttatatataa tctgcaaaaa aattatgaaa
     7501 gtgcagcttc ccatattgct gtgtgtaatg caataacgct acagcaaata aaacctgaaa
     7561 ttgaaaaagc acaagctgct caaaaagata ataataccaa tgttgctatg gctcattata
     7621 aaaagatact tgagataaat cctgtaaaca gggaagcgta tgtacagctt tcccattatt
     7681 acagaataca gaaaaaaatt gatatggcta tccagattct tgaagattgc aaaaaagtat
     7741 atcctaaaga tactgatgtt cttttgacac ttgcccatat gtattttgag tttaaacatc
     7801 caaaacgtcg tgagtatttt atcaagcagg caattgacct gtgcaaagtg gccattggaa
     7861 ttgacagctc gcactgtgaa gcatattctt tgctgtttga aatatataaa gagttaggtg
     7921 aagtcccttt gcgggatgag catgctgcca aatatgatgc atgccttggg ggaagcagat
     7981 aaagtttaat caaacactat tgttttattt gcataaacca gtaccttatg ttccaaatgt
     8041 tttttaagtg ctcgggacag taccagtttt tcaatatctt tgcctttgcg tatgatatct
     8101 tcaattgaat ctttatgcga aacccgtata acatcctgct caataatagg cccctggtct
     8161 aacaattcgg taacatagtg actggtggct ccaatgattt taacaccatg ctgatatgcc
     8221 tggtggtatg gttttgcacc tttgaatgct ggcaaaaatg aatggtgtat attaataatt
     8281 ttgttaggat aatgtgaaac aaaatgcgca gatagtatct gcatatagcg ggcaagcaca
     8341 atgcaatcaa cgtcaaaatc tttaagtacg gctaattctt tttgttcctg ctgtagcttg
     8401 ttttcttttg ttttttggaa aacaaaaaaa ggaatgttaa aatatttggc gatagttcct
     8461 aatgattcat gattgctgat gatacaggca atatcagtgg caatttcacc ttcttttgcc
     8521 ttaaggagaa tatcatacag gcaatggtcc atttttgaaa caaagattga tgtacgttgc
     8581 tgttttgctg agaaatgtaa ttgccagttc atgctgtatt cagcagcaat tggctcaaag
     8641 gcttttataa tcttgttttt ttctatggca aaaccatcca agtcccattc aatacgcatg
     8701 aaaaaagttc ccgactcttc atctgcatgc tggtcagatc gtaaaatatt accattattg
     8761 gaatatataa aatgtgatat tttataaacg atcccctttt tatcaggaca tgagataagt
     8821 aaaatagcac tttctttaga atgcacagga aaaccccatt aagtgtttat tggttaatag
     8881 atgtgctgtt atttatttat tgtcaatgaa ttattatgct ctgacccctt ctcttttttt
     8941 ttacacccaa tccactacat acgaatattt tttccg
//
LOCUS       DAORYL010000102         8322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_6204_length_8322_cov_11.663965, whole genome shotgun sequence.
ACCESSION   DAORYL010000102 DAORYL010000000
VERSION     DAORYL010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8322
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6204_length_8322_cov_11.663965"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..1679
                     /locus_tag="PLV81_13695"
     CDS             222..1679
                     /locus_tag="PLV81_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase family
                     protein"
                     /protein_id="HOM11191.1"
                     /translation="MPLKTPAEYIESIKALKRTVYLFGQKIDNTVDNPIIKPSLNAVA
                     MTYALAQKEEYRDIMTATSHITGNVINRFTHIHQSIDDLVKKSKMGRLLGAKTGCCFQ
                     RCVGMDALNALSMTTYAIDQKYGTQYYQRFLKYLQYVQDNDLVCDGAMTDPKGDRSLP
                     PHKQEDPDLYLHVVKETKDGIIVRGAKAHQTGALNSHEVIVMPTIAMRPEDRDWAVSF
                     AVPADAEGIIYIYGRQSCDTRKLEKYSIDQGNALFGGHEALIVFDDVFVPWDRVFMYK
                     EYDFAGHLVERFASYHRQSYACKVGVGDVLIGATQTIAEYNGIDKASHVKDKIIEMIH
                     LNETLYCGCIACATEGKREEPGTYMVNTLLANVHKQNITRFPYEIARLAQDIAGGALV
                     TLPSADDLNHPEAGKWIKKYFKAKSDIPTEHRIRILRLIENITMGTAAVGYLTESMHG
                     AGSPQAQRIMIARESNVKEKQKTAQRLAQVISSDE"
     gene            1869..2315
                     /gene="tnpA"
                     /locus_tag="PLV81_13700"
     CDS             1869..2315
                     /gene="tnpA"
                     /locus_tag="PLV81_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOM11192.1"
                     /translation="MSTYTQILYQLVFSTKYQEMILLKENRPKLYQYITGILKNKNCH
                     LYQINGIDNHVHILFHLHPSQSLSDLVKDIKLASSKFIKENHLFPNFIGWQNGYGAFT
                     YSIEAKDNLIAYIKNQESHHKIKSFRDEFIELLVEHGIIFDEKFVL"
     gene            complement(2485..4458)
                     /locus_tag="PLV81_13705"
     CDS             complement(2485..4458)
                     /locus_tag="PLV81_13705"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOM11193.1"
                     /translation="MKKLLILLLAGFVACATGANTTQTKSLSKDLKNLVNKSCFEVVV
                     NKPEKDTLTYEKELPWHLIPYNIRVDKYYSIGTAFAISPTELLTAFHVLDLKTDSLIY
                     KDFYIRDSQGNVYEVDKILAFDSHRDFIRFTVKNKTFAKYLKLKENYTIGESVFAVGN
                     AYGEGIVMRQGELIGTYPEPEDGKFNLLKSSSDVNFGNSGGPLIDNQANCIGIVIQRK
                     DNIAYSLPVAEVLKIGNRGIFHVRMNFGFTLLPEKTMVKDFDDELSLPKGYKEIKKLF
                     NERFKKFYVSTMDELLKGQGRGMFPEGEESLIPLFDFTDSYFLQVNYLSKDNNKWTIS
                     NIEYKETKIHGNGVIRMANPDKRLLFIDISRPDDVSLQDMLKDPKVGMKLVLEGQSIT
                     RDFGDVKTRILSLGDPLAVEYHTDTYGRKWRLCTWVLEYSDEAVISCSTPTPEGMSAV
                     VFVCETSEIEFWKYDALKLLDYVQISYYGRLKDWKEFLTGNTLLPKALSGVKFDYSVQ
                     RGFQFGAGMFSISATNDVIAINDDVRMGINFGYIKQATGTEWAIRRVMLSEYQKDNYF
                     VLVNWVAPDTRLPKDYINYWESIVNAEHPYTMKPFTSDNSTKIAAVVKKIQEKPEKVK
                     KLYTLFLGKAGKVEEDRMIQDFKKLMNGIVINE"
     gene            4676..5662
                     /locus_tag="PLV81_13710"
     CDS             4676..5662
                     /locus_tag="PLV81_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11194.1"
                     /translation="MPKGNTQKPIALILAGFDKPDRKTMIKRKKQLIEAYDGDVIYIG
                     KNKFLQTLAGKPVIQYVLDAIYHAKKHGKPLYEKIYVYNDVQNFTDAIDTKKYKNLTV
                     LQMKQSVGGHLKDFYFNYIDYGQRLDIFFGDTPRITTKDVEWINREYDSILGKAKDYR
                     GAIVHLIYSIVEYPDVAKDTWLPHRMKAIKRGEYKGKHKYFVGFDTFEARIGNSAAII
                     KHHSIDPIVEYQIIDFFYGLRKALSPNIFSKILYHLWKTKNFDVIRQVKQRCLEEEKF
                     YRAVIDVIERVYKLDLSNFGGYIYHIRNNAAHWENDIDSPKDLKAFRAHMEK"
     gene            5861..6067
                     /locus_tag="PLV81_13715"
     CDS             5861..6067
                     /locus_tag="PLV81_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11195.1"
                     /translation="MIDNNGLIEKMMQKNFNRCCELTQAMFSLKHALYKSLFPTLSDE
                     EIWSKITEEILKRNHNHEKPTINS"
     gene            6042..>6259
                     /locus_tag="PLV81_13720"
     CDS             6042..>6259
                     /locus_tag="PLV81_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11196.1"
                     /translation="MKNQPSIHEYIITISSCFINTNIPFCIAGGIAVSLWGHVRATED
                     LDVIALIDETHDFLKRCIQNRKLFSVKG"
     assembly_gap    6260..6359
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6421..>8322
                     /locus_tag="PLV81_13725"
     CDS             6421..>8322
                     /locus_tag="PLV81_13725"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HOM11197.1"
                     /translation="MKQKPIHTVLSITLVICALAFLSCWDTTQTIDQDEKQPQINLRA
                     SVLEIPSSGTYDFGQIEVGQTKSAVITIENLGQKFLELTGPITLSGVNASLFDIENQP
                     NTTIPPFTYTTFTISFTPDSQGIKTATITITSNDPQKTTFTFTIQGEGIVITAPEINI
                     QYSGNNVNKGDSIHLGSCVIGNSSDFTLSIQNNGTADLTIQAPIISGIDQSLFAITTA
                     PANTVAAGSSTDFVVRFTPTSAGNKTAIITITNNDADEGTYSFTITASATTVPQPDIF
                     VMQDATPLYNTIGIFDFGQVNQGQTSSSVTFTIKNTGTANLTLTTPFTISGTDASMFS
                     VSPPGSTTIVPNDITSFSMTFAPSSAGEKNATVSIANDVPGKNPYTFSIKGTGIAIPE
                     IDIDQSGTPIANNGTFDIGSTTVNVSKDTLFTIYNNGLGILNLPTTPAVTLTGSPFFT
                     LVSYPGNTISPSSSSNFTLRYLPTEAGNHSTILTITNNDLDENPYIITITGQATSVPT
                     PKLEITKSSQVLLNNSSVDFGGMTVGGTPIDVTFTITNKGDSNLSITGTTISPGDFSI
                     VTAPTTPVTPGGTTTIIVRFLATSAGTKVAALMIGSDDLNNNPFTINLTGKGLEPEIN
                     CKINGSDFSS"
BASE COUNT         2701 a         1627 c         1411 g         2483 t
ORIGIN      
        1 tcaacataaa tggacatata aaatgggata aataaaaagt ttctaatatc caatagatat
       61 attattatat tataaaaaaa ttttttagaa aactctttgt tggcagtaaa ttctattaag
      121 caattcaatt attataatac atttattatt gacaaactta ataaagtaat gtaaatttgt
      181 atttatgaat gtacattaac cttttagaaa ggagattacc tatgccactt aaaacccctg
      241 ccgaatatat tgaaagtatt aaagcattaa agcgaaccgt gtatcttttt ggtcagaaaa
      301 ttgataatac cgttgataat cccatcatca aaccatcact taatgcggtt gctatgacgt
      361 acgcccttgc tcaaaaagaa gaatacagag atatcatgac agctacatca catataaccg
      421 gcaatgtcat aaaccgtttt acccatattc atcagagcat tgatgatctg gtgaaaaaaa
      481 gcaagatggg gcgtttgctt ggagcaaaga cgggatgctg cttccagcgc tgtgtaggta
      541 tggatgcgct caatgcacta tcaatgacaa catacgcaat tgatcagaaa tacggcacac
      601 aatactatca gcgcttttta aaatatttac agtatgtaca ggataatgac ctggtatgtg
      661 atggtgcaat gaccgatccc aaaggagacc gttcactgcc acctcataaa caggaagatc
      721 ctgatctgta cctgcatgtg gtaaaagaaa ctaaagatgg cattattgtg cgcggtgcaa
      781 aggctcatca aaccggtgct ctgaattcac atgaagttat tgttatgcca actatcgcca
      841 tgcgccctga agacagggac tgggctgtgt cctttgcagt accggctgat gctgaaggga
      901 ttatttacat ctatggacgg cagtcctgtg acacccgtaa gttagaaaaa tatagcatag
      961 atcagggaaa tgccctgttt ggtggtcatg aagcgctgat agtatttgat gatgtgtttg
     1021 taccctggga tagagtcttt atgtacaaag aatatgactt tgcaggtcac ttagtagaac
     1081 gctttgcctc ataccaccgc cagagttatg cctgcaaggt tggcgtggga gatgtgttaa
     1141 ttggtgcaac acagacaata gccgagtaca acggcattga caaagcatcg catgtgaaag
     1201 ataagatcat tgagatgata cacctgaatg aaacgctgta ttgcggatgt attgcctgtg
     1261 caacagaggg caagcgtgaa gagcccggca cctatatggt taacaccctg cttgccaatg
     1321 tgcataagca gaatatcacg cgctttccat atgagatagc acggttagct caggacatag
     1381 ccggtggtgc actggttacc ctaccatcag ctgatgacct caatcatcca gaagcaggca
     1441 aatggattaa aaaatatttc aaagcaaaaa gcgatatccc taccgaacac cgcatacgta
     1501 tcctgcgctt gatagaaaac attaccatgg gaacagctgc ggtaggatat ttaaccgaat
     1561 caatgcacgg agcaggttcg ccacaggcac agcgcattat gatagcacgc gaatccaacg
     1621 tgaaggaaaa gcaaaagaca gcgcagcgcc tggcacaggt aatttcttct gatgaataat
     1681 attttttttc aatcgcgaag cgattaaata tgaatagcca tggatgtaat ccgtggctat
     1741 tcaccatcaa tacacaaaac cccaaagggg tttaatatga ttgacagaat ttttaatgta
     1801 ctatacatct tcaatatatt aaatataaac cataatatct caaagcatta caaccaataa
     1861 aatttgatat gagtacatac acacaaatat tatatcaatt agtattcagc acaaaatatc
     1921 aggaaatgat attattaaaa gaaaataggc ctaaattata tcaatatatt actggtatat
     1981 taaaaaataa aaattgccac ctataccaaa taaatggcat agacaatcat gttcatatat
     2041 tatttcatct tcacccgtcg caatcattgt cagatttagt aaaagatata aaattggcaa
     2101 gttcaaaatt tattaaagaa aatcatctat tcccaaactt tataggatgg cagaatggtt
     2161 atggagcatt tacttactca attgaagcaa aagataatct tatcgcttat ataaaaaatc
     2221 aagaatcaca tcataaaata aaaagtttca gagacgagtt tattgaactt ttagtagaac
     2281 acggaattat ttttgacgaa aaatttgtat tataaaattt caacccttcc agggttgaaa
     2341 aagtttttga tttattttct ccgcattaca tacggagcta ttactattta atcccttcgg
     2401 gattatagta aaactatctt tgtatttaca cttaaatctt aacatatagc cgcgtgacac
     2461 atacgcggct ataattgtat tcaattattc attaataaca atcccgttca tgagtttttt
     2521 gaaatcctga atcattctgt cttcttccac ttttccggct ttccctaaaa acagcgtgta
     2581 cagcttctta actttttcag gtttttcctg gatcttctta accacggcag ctatctttgt
     2641 ggagttatcg cttgtaaatg gcttcatggt atacggatgc tcagcattga caatgctttc
     2701 ccagtaatta atataatcct ttggcaacct ggtatcgggt gcaacccagt ttaccagtac
     2761 aaagtaatta tctttctgat attcactcaa catgacacgg cgtattgccc attctgtgcc
     2821 tgtggcctgt tttatatatc caaaattgat acccatacgc acatcatcat tgattgcaat
     2881 gacatcattt gttgccgaaa tagaaaacat acctgcacca aactggaatc ctctttgcac
     2941 tgaataatca aacttgaccc cgcttaatgc ttttggcagc aatgtattac cagttaaaaa
     3001 ctctttccag tctttcaatc ttccataata tgatatctgt acataatcca ggagtttcag
     3061 cgcatcatat ttccagaatt caatttcaga tgtttcacat acaaaaacca ccgccgacat
     3121 gccttcaggt gtaggtgttg aacagcttat gacagcttca tcagaatatt ccagcaccca
     3181 tgtacacagc cgccatttac ggccgtatgt atcggtatgg tattcaactg ccagtggatc
     3241 acccagtgat agtattcgcg ttttgacatc gccaaaatcg cgggttatac tctgtccctc
     3301 aaggacaagt ttcatcccca ctttggggtc tttcagcata tcctgcaagc tgacatcatc
     3361 agggcgcgaa atatcaataa acagcaaacg cttatcagga ttagccatac ggataacgcc
     3421 attgccatgt atctttgttt ctttatattc aatattggag atagtccatt tgttgttatc
     3481 cttgcttaaa taattcacct gtaaaaagta actatcggta aaatcaaaaa gtgggataag
     3541 cgattcctca ccctcaggga acattcctct gccctgccct ttcagaagtt catccattgt
     3601 tgaaacataa aattttttaa aacgctcatt gaacaatttt ttaatttctt tatatccttt
     3661 tggaagcgat agttcatcgt caaaatcctt caccatagtc ttttccggaa gcagcgtaaa
     3721 accaaagttc atacgcacgt ggaaaatacc acgattgccg atttttaaaa cctcagccac
     3781 tggcaatgaa taggcaatat tatctttacg ttgaataaca atgccaatac aattagcctg
     3841 attatcaata aggggaccgc cactgtttcc aaaattgaca tcggatgagc ttttcaataa
     3901 attaaatttt ccatcttccg gttcaggata tgtgccaata agctcgccct ggcgcatgac
     3961 aataccttca ccataggcat tgccaactgc aaagacactt tcgccaatag tataattttc
     4021 ttttagtttt aaatattttg caaatgtctt atttttaact gtaaagcgta taaaatcccg
     4081 gtgactatca aacgctaaaa ttttatcaac ttcatagaca ttgccctgtg aatcacggat
     4141 gtaaaaatct ttatagatga gcgaatcagt cttcaaatcc agcacatgaa aagcagttaa
     4201 aagctcagta ggggagatag caaatgcagt cccaattgaa tagtatttat caacccgaat
     4261 attgtacggt atcagatgcc atggcagctc tttttcgtat gtcagggtat ctttttccgg
     4321 tttattgaca acaacctcaa agcatgattt attgacaaga tttttaaggt ctttggataa
     4381 ggattttgtc tgagtggtat ttgccccggt agcacatgcc acaaatcctg ctaacagtag
     4441 tatcaacagt tttttcattg gtacctctat attctttttg cagagtttag taaagttcag
     4501 ggtttatttc ttcatattta atataccggc tgtcaagtta aattgcaatt atatttatat
     4561 ttttattccc accttattca tcactatggc attacagata gttactatta aaggcttgac
     4621 tgatacgtgt catgcttata ctttagtatt aataattgta tagtcggata ctatcatgcc
     4681 aaaaggaaat acacagaagc caattgccct catccttgct ggctttgaca agcctgaccg
     4741 taaaaccatg attaagcgta aaaagcagct cattgaagct tatgatggcg atgttattta
     4801 tattggcaaa aataaatttt tgcaaacact tgccggcaag cctgttattc agtatgtgct
     4861 tgatgccatc taccatgcca aaaaacacgg taaaccactg tatgaaaaaa tttatgtata
     4921 caatgacgta cagaacttta ccgatgccat agatactaaa aaatacaaaa acctcactgt
     4981 gttgcagatg aaacagtcag ttggcgggca tttaaaagat ttttatttca actacattga
     5041 ctatggccag cgccttgata ttttttttgg tgataccccg cgcatcacca caaaggatgt
     5101 tgagtggatt aaccgtgaat atgatagcat tttaggaaag gcaaaagatt accggggggc
     5161 gatagttcat ttaatttaca gcattgtgga atatccggat gttgcaaaag atacatggct
     5221 tccgcaccgt atgaaagcaa tcaaacgcgg cgaatataag ggcaaacaca aatactttgt
     5281 tggctttgat acgtttgaag cacgcattgg caactctgct gctattatca agcatcactc
     5341 aattgatcca atagttgaat accagattat tgactttttc tacggattac gaaaggcact
     5401 atcgcccaat atcttttcca aaatactgta ccatctatgg aaaacaaaaa attttgatgt
     5461 catacgacag gtaaagcaac gctgtcttga ggaagaaaaa ttttaccgtg ctgttattga
     5521 tgttattgaa cgggtctaca aactggattt atctaacttt ggcggatata tctatcatat
     5581 caggaacaat gctgcccact gggaaaacga tattgacagc cccaaagatt taaaagcatt
     5641 cagagcacat atggaaaagt gatgcttttt tacaattgac aacacgtgct ttgcaagata
     5701 tattccaatt ataaaacatg tgtacaacaa tgtactggtt ggtgaataag ctgttgggta
     5761 ccatttacag ctttggtatg ctctttgcaa aatttaatca cacataaatc cttctttaag
     5821 gaagggtaag cgttttagtg tatagaatgg attatgatgt atgatagata ataatggttt
     5881 aattgaaaaa atgatgcaaa aaaacttcaa ccgctgctgt gaactcaccc aggccatgtt
     5941 ttcattgaaa catgcactgt ataaatcatt atttcccact ctcagtgatg aggaaatatg
     6001 gtccaaaatt acagaagaaa tacttaaaag aaaccataat catgaaaaac caaccatcaa
     6061 ttcatgaata cattattact ataagctcgt gttttattaa taccaatatc cctttctgca
     6121 ttgctggagg tattgctgtc agcttatggg gtcatgtaag agcaactgaa gatttagatg
     6181 ttattgcttt aattgatgaa acacatgatt ttttgaaacg ctgtattcaa aatcgcaaac
     6241 tattttcagt gaagggaatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     6361 aaatttttta tttcacaata tcaagtaatt attgtactat gtatatagca attattgtgt
     6421 atgaaacaaa aacctataca tactgtttta agcataacgt tagttatatg tgctttagct
     6481 tttttatcct gctgggatac gacgcaaact attgaccagg acgaaaagca gcctcaaata
     6541 aacctacgtg ccagtgtgct tgaaatacct tcatcaggaa cctatgattt tggacaaata
     6601 gaagttggtc aaactaagtc agctgtaata actattgaaa acttaggtca aaaattttta
     6661 gaacttacag gtcccataac attaagtggg gtaaatgcct ctttatttga tattgaaaac
     6721 cagccaaaca ctaccattcc accttttacc tatacaactt ttactatttc ttttacccct
     6781 gattcacagg ggataaaaac agccacaata acaattacaa gcaatgatcc tcaaaaaact
     6841 acgtttactt ttactataca gggtgaaggg attgttatca ctgcacctga gataaatatt
     6901 caatatagtg gaaataatgt aaacaaaggt gattctattc atttaggtag ttgtgtaata
     6961 ggaaattcat ctgattttac tctttccatt caaaataatg gcacagctga cctcaccata
     7021 caggcaccaa ttataagtgg aattgatcaa agtctatttg ctataaccac tgctcctgcc
     7081 aatactgtag cagcaggttc ttcaacagat tttgtggtca gatttactcc aacatccgct
     7141 ggtaacaaaa ctgctattat taccataacc aataatgatg ccgatgaagg cacatatagc
     7201 tttacaataa cagcttcagc aacaacagtt ccccagcctg acatctttgt catgcaagac
     7261 gctactcccc tgtataacac cattggtata tttgattttg gccaagttaa tcaaggacaa
     7321 actagctctt cagtaacatt taccatcaaa aatactggta ctgcaaatct tacattaact
     7381 actcctttta ctatatcagg aactgatgct tcaatgtttt ctgtttcacc tccaggatca
     7441 acaaccattg taccaaatga tataacatca ttttcaatga cgtttgcgcc gtcttcagca
     7501 ggtgaaaaaa atgcaacagt ttccattgct aatgacgtgc caggaaaaaa tccatatacc
     7561 ttttcaataa aaggtactgg tatagctata ccagaaattg atatagatca aagtggaacc
     7621 ccaattgcca ataatggaac ttttgacatt ggctcaacaa ctgttaatgt ttctaaagat
     7681 actctattta ccatatacaa taatggcctg ggtattctta atcttccaac cacccctgct
     7741 gtaaccttaa ccggttcccc tttttttaca cttgttagct atccaggcaa tacaattagt
     7801 ccatcatctt catcaaattt tacgttacgc tatcttccta ctgaagccgg caaccattca
     7861 acaatattga caattaccaa caatgatctt gatgaaaacc catacataat cacaattacc
     7921 gggcaggcaa cgtctgttcc aacaccaaaa cttgaaatca ccaaatcatc acaggtgtta
     7981 ttgaacaata gttctgttga ttttggcggc atgaccgttg gtggaacacc aatagatgtt
     8041 acctttacca taactaataa gggcgatagt aacctttcaa taactggcac aacaatttcg
     8101 cctggtgatt tttcaatagt aactgcccca acaactcctg taacaccagg tggaacaaca
     8161 acaatcattg taagattttt agctactagc gcaggcacaa aagtagctgc tcttatgata
     8221 ggaagtgatg atctaaataa taaccctttt actattaacc ttacaggtaa aggtcttgaa
     8281 cctgaaatta attgcaaaat aaatggctct gattttagca gt
//
LOCUS       DAORYL010000103         8254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_6254_length_8254_cov_7.470179, whole genome shotgun sequence.
ACCESSION   DAORYL010000103 DAORYL010000000
VERSION     DAORYL010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8254
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6254_length_8254_cov_7.470179"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1036)
                     /gene="cas6"
                     /locus_tag="PLV81_13730"
     CDS             complement(110..1036)
                     /gene="cas6"
                     /locus_tag="PLV81_13730"
                     /inference="COORDINATES: protein motif:HMM:NF021551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR system precrRNA processing
                     endoribonuclease RAMP protein Cas6"
                     /protein_id="HOM11198.1"
                     /translation="MLPLYKFKIYIRATHDIEFYNFPGIALRGGLGHILKTTVCVFKS
                     IDAVCKKCVLVQTCVYANAFESLNYSDTKVMKKATHLPHPFVITPVIKNKKINAGDEF
                     NIYLTLIGKGITYFPHFVYSLNKLGEKGINKNKGTYSILKIVNIVDNSEICDYKTLSI
                     ENNLKPFDSFENYGEIKSIKINFITPCKIMQNKKEVNNISFEFLMKTILRRIKILSII
                     YNDKLITENFNELIEQSKNNIIKKSNIHWVLNERYSKRQNNKMLLKGFTGYVIYEGNF
                     KIFYPFLKIAEIVNIGKNTSFGYGALKVSVID"
     gene            complement(1067..2263)
                     /gene="cmr6"
                     /locus_tag="PLV81_13735"
     CDS             complement(1067..2263)
                     /gene="cmr6"
                     /locus_tag="PLV81_13735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr6"
                     /protein_id="HOM11199.1"
                     /translation="MPVFHPFNREARNFDFSKGNYGLWYTKYIPVSCDEQNFAKASDD
                     KGDTTNAVNYYFKRYNVTNNVLLNELSNRHVQQKEFMKAMEKNYHAIELRVKLISPLL
                     TGVGYPHPSESSLLFEHTLGIPYIPASSIKGVVRFAFTIGLLDDDIYGTENYYGTDKD
                     GKRFLKEELTPVKDAFGYSADNNSETNNKEIIKSSRGKVIFLDAYPEKIPGLIVDILN
                     PHYPEYYSGNKPPADNQDPLPVKFLAVARDTVFLFRVLVKKVNDELLVLVKNAIERAF
                     FEEGVGAKTTIGYGRFKFAEQSQDNKLGIKNNKKDEQTQEIEIIQGNKYVAQIVGITQ
                     DGGSVQVIMPNGQKTIIGKKQFPKNIQNNLKQYLKKGMELNVEVVSYQSGNIVLKLID
                     ETIKSK"
     gene            complement(2273..2635)
                     /gene="cmr5"
                     /locus_tag="PLV81_13740"
     CDS             complement(2273..2635)
                     /gene="cmr5"
                     /locus_tag="PLV81_13740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated protein
                     Cmr5"
                     /protein_id="HOM11200.1"
                     /translation="MTTLGQDRSKYALEQVLQVGQRIDKFDTFTAGAATMILRNGLGQ
                     TMAFWMQKSEGRDDEKHGALLRIVRVWLNEKQIIQSTDNISFIRALSEVPQETYLAAQ
                     KETLALLEWVKRYASSNF"
     gene            complement(2635..3534)
                     /gene="cmr4"
                     /locus_tag="PLV81_13745"
     CDS             complement(2635..3534)
                     /gene="cmr4"
                     /locus_tag="PLV81_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr4"
                     /protein_id="HOM11201.1"
                     /translation="MFKTEKISILKFENISPLHAGSGNALSAVDLPIQRERHTNWPHV
                     QASALKGALRAHFRDFNEEKANFPSARFLCNIIFGSDSQDSWDSNNNEEESLPGAISV
                     SDARLLAFPVRSNFAPFVRITSPAVIERLKKDLEFADYSPDITVPSVENNKALALNWD
                     INNQRCIIEDAVVEIEGPIKIDAINNFINEKHRVLIVSDAMYDYCVSSCTEIQAQIKI
                     DADTGTTQDGSLRYQELLPSDSILYALIAFGKQKYAANEFQVQTICEYFEKVFSEGSF
                     VQIGGDETLGRGICKISWIKGGK"
     gene            complement(3544..4497)
                     /locus_tag="PLV81_13750"
     CDS             complement(3544..4497)
                     /locus_tag="PLV81_13750"
                     /inference="COORDINATES: protein motif:HMM:NF021236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated Cmr3 family
                     protein"
                     /protein_id="HOM11202.1"
                     /translation="MKWFSIEPLDTLFFRGAEPMVMGSDHSATLIFPPHTETISGALR
                     TACIEATGVSFADYNEGKAPEDLLKKIGQSGSAAPFTVIGPLFTMNEMLFLPAPYTWF
                     VEKHSMEKANISDIYPNVKVIIKNLYELSNDLVIVNGQSQIHWVKSGVQDLITAGGMW
                     VSSEDFGKDKVSLYPIEHFFVKERRTGIALNEQKSVRSGHLYSFVHARLKKGVKIVCG
                     IDKDIGLANSFHIRLGAEQRMCLVNSMPAPVIPSGRNDIFMALSLIEGNEQSNSVLIA
                     GGKPIYAGGWDMHRRFHKPMKGYYPAGSVFSAKISENMIQI"
     gene            complement(4494..7145)
                     /gene="cas10"
                     /locus_tag="PLV81_13755"
     CDS             complement(4494..7145)
                     /gene="cas10"
                     /locus_tag="PLV81_13755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR-associated protein Cas10/Cmr2"
                     /protein_id="HOM11203.1"
                     /translation="METNALYWNDKLAAFFHDPFDKALNIPGHEKRASQYLEAFGLKP
                     PEDKFWKMADILAAGFERGVFPGYQADVQKSGAVDFLKHAIITHPVSSNDKLDVLLAV
                     DSVNQEEIIKLIESDMQVISQGDEIKKARNRFFYVYFLLRYRLSSKNIGGLGALWHRL
                     PADTRIPDHSIWHHNALVSSLYSSMELAKAEEGIGLAVFSITPVQGFIAKARKLRDYW
                     TSSVLLSWLAFEGIRYVIEQLGPDHVLYPSLLDQPLVELYLKKIPGFDSLIQESCLPK
                     PLEIATLPNKFLFVLPFGKANEFCEAIKQTVIMRWNELCDLIQNSVAERCIKEVEDKK
                     VFFKIFKRQTEHFWDIDFACTKLVGERDIAKLNKFFEEEVYSSHNKLSKLFNTLVEGS
                     SQGCFYTVSHRLAQSALASAKIKRKIRRNNENGEKCHLCGQFEVLHTIAHPENLTAKQ
                     YVHKIKIFWDNLKNAYSEYDFNEGEKLCAVCLVKRLAAQVTKEKQHLLSGVFENADAF
                     PSTTEMALNGYFKRCGIDDKNKRWLYAQQLHQKDEFEREIKKELKQKLREEDKYLAIL
                     LMDGDNMGSLINGESVAARWETVLHPEVIEKIQRKTFEERYVKIWNEIFNDSNIKKRM
                     LSPALHATISEALSDFSLFSVKNIIEQFSGRLIYAGGDDVCAVLPAFNALPAAREIQK
                     MYSQPFVFIDDMGKPAIVEKNWEPQPGKITMHLGKGEKISISAAILICHHKESLSEMI
                     SRAHSLLELYAKEMCGRNACAIELKKRSGGSRYFAAKWDNLVWESFQNLIMLLGTKGT
                     KERLSTSLIYRLDEMRDGIIPLIEKDESLQLYRKFVQKLLTKSLFSEQDPNIENISSM
                     IVQTTYMNKKFVPEGLIIANFLAGGEQ"
     gene            complement(7156..8154)
                     /gene="cmr1"
                     /locus_tag="PLV81_13760"
     CDS             complement(7156..8154)
                     /gene="cmr1"
                     /locus_tag="PLV81_13760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr1"
                     /protein_id="HOM11204.1"
                     /translation="MAFYIEKHCGRIKRSYLIEVVTPLFISGADTQISELRASSLKGL
                     LRFWWRAINPINDLNNSQNKEGKIFGSTMNDSSCKSSFSLMIEPKEVKIEQNMPKGKM
                     VAVNSSKKQFNIDIFTYLGIGIYDPKTRQWARKNIAPGSTFIIHCTYDSESIKDEIEK
                     ALSALIHYGGLGAKSRNGFGSLFSKDVPYINFLDKNKNVNVLPYTTFSSKSKIFLFNE
                     QKNWIDALSDIGIAYRQMRLSTEMDRKHDYRNRKYIGLPIVQANNEEVKKGRHAKPCF
                     LHVSKNEKGYKGQILILPYNYSKPLNNRNNEYDNTIKKMIYIFEKIQKNEKMTTIY"
BASE COUNT         2612 a         1587 c         1142 g         2913 t
ORIGIN      
        1 ttaactactg gagacagcaa tgaagctact cattgagatt tggggaaatt atcacgcctg
       61 gaaagaagtt acttacacgt ataatgatga gatctaatat aaatgattac taatcaataa
      121 cacttacctt taatgcacca tacccaaatg atgtattttt ccctatattc actatttctg
      181 ctatctttaa aaatggatag aatattttaa aattcccttc atatattaca tagcccgtaa
      241 aaccctttaa caacatttta ttattttgtc tttttgaata acgttcattc aatacccaat
      301 gaatattcga ttttttaata atgttatttt tggattgttc tatgagttca ttaaagtttt
      361 cagtaattaa cttgtcattg tatattattg ataaaatttt gatacgcctt aatatagttt
      421 tcataagaaa ctcaaaagat atattattaa cctccttttt attttgcata attttacagg
      481 gagttataaa atttatttta atacttttta tttcaccata attttcaaaa ctatcaaaag
      541 gctttaaatt attttctatg gatagcgttt tataatcaca aatttctgaa ttatcaacaa
      601 tgttaactat tttaagtata gaatatgttc ccttattttt attaatacct ttttctccca
      661 atttattcaa tgaatacaca aaatgaggaa aatacgttat acctttccct attaaagtta
      721 aataaatatt aaattcatca cccgcattaa tttttttatt ttttattact ggagtaatta
      781 caaaaggatg tggtagatgt gttgcttttt tcatcacttt tgtgtctgaa taattcaggc
      841 tttcaaaagc attagcataa acacatgttt ggactaaaac gcactttttg cataccgcat
      901 ctattgattt aaaaacacaa acagttgttt ttaaaatatg ccccaatcca ccacgcaaag
      961 caatacctgg aaaattgtaa aactcaatgt catgggtagc tctgatatat atcttaaatt
     1021 tatatagtgg caacatgttt tgcagcaagg atagcattaa ttcaagttat ttacttttta
     1081 ttgtttcatc aatcaattta agaactatat taccactttg gtaagataca acttcaacat
     1141 tcagttccat acccttcttt aagtattgtt ttaaattatt ctgaatattt tttggaaatt
     1201 gctttttccc aattattgtc ttttgcccat taggcattat cacctgaacg gaaccaccat
     1261 cttgagttat tcctacaatc tgggcaacat atttatttcc ttgtataatt tcaatttctt
     1321 gagtttgttc atcttttttg ttattcttaa tacctaattt gttatcctgt gattgttcgg
     1381 caaatttaaa tctaccataa ccaatagttg ttttagctcc aacaccttcc tcaaagaatg
     1441 cacgctctat tgcatttttt actaacacaa gcaactcatc attaaccttt ttaacaagaa
     1501 ctctaaacaa aaaaacggta tcccttgcaa cagctaaaaa tttcaccggt agtggatctt
     1561 gattatccgc cggaggttta ttaccgctat aatattcagg gtaatgaggg ttgaggatat
     1621 ctactattaa accaggaatt ttttcaggat atgcatctaa aaatataacc ttaccacgac
     1681 tggatttaat tatttcttta ttattcgttt cactattatt atctgctgaa tacccaaagg
     1741 catctttaac gggtgtcagc tcttccttta aaaaacgctt tccgtcttta tcagttccgt
     1801 aatagttttc agttccataa atatcatcat ccaatagtcc aatagtaaat gcaaatctta
     1861 caaccccttt tattgaagaa gctggtatgt acggtatacc taatgtatgt tcaaaaagca
     1921 gtgaagattc agatggatga ggatacccta caccagttaa aagcggagaa atgagtttaa
     1981 cacgtaattc aatagcatga taattcttct ccattgcttt catgaattct ttttgttgga
     2041 catgccggtt tgaaagctca tttaatagaa cattattcgt tacgttatat cttttaaaat
     2101 aataattaac agcatttgta gtatcgcctt tgtcatcaga tgctttggca aagttttgtt
     2161 catcacacga tacaggtata tattttgtat accataaacc ataatttccc tttgaaaaat
     2221 caaaattacg agcctctcga ttaaatggat gaaaaacagg catatacacc ccctaaaagt
     2281 tagaacttgc ataacgcttt acccattcca aaagcgcaag agtttctttc tgtgctgcaa
     2341 gatatgtttc ctgcggaacc tcactaagcg cccgaataaa agatatatta tcagtacttt
     2401 ggataatctg tttctcattc aaccaaacac gtacaattct taacagagca ccatgctttt
     2461 catcatctct accctcactt ttctgcatcc agaatgccat agtttgacca aggccattcc
     2521 ttaaaatcat agtagcagca ccagcagtaa aggtatcaaa tttatctatt ctttgaccaa
     2581 cttgaagaac ctgttcaagt gcatatttag aacgatcctg gcctagcgtc gtcattattt
     2641 acctccttta atccaggaaa ttttgcaaat acccctgccc agtgtttcat cacctccaat
     2701 ttgcacaaaa ctaccttcac taaatacttt ttcaaaatat tcgcatatcg tttgtacctg
     2761 aaattcattc gctgcatatt tttgtttacc aaatgcaatt agcgcatata aaatagagtc
     2821 agatggtaaa agctcctgat aacgcaatga tccatcctgt gttgttcctg tatccgcatc
     2881 tatcttaatc tgagcctgaa tttcagtaca tgaagatacg caataatcat acattgcatc
     2941 tgaaacaatc aatactctat gtttttcatt aataaaatta ttaattgcat caatttttat
     3001 tggtccttca atttcaacaa ccgcatcctc aataatacat ctttgattat ttatatccca
     3061 gttcaatgcc aatgctttat tattttcaac cgaaggtaca gtaatatcag gagaataatc
     3121 tgcaaattct aaatcttttt taagcctttc tattactgca ggggaagtta tcctcacaaa
     3181 aggtgcaaaa tttgaacgaa caggaaaagc taaaagacgc gcatcactta ctgaaatagc
     3241 cccgggcagc gactcttctt cattattatt gctatcccaa ctatcctgtg agtctgaccc
     3301 aaatataata ttgcatagaa aacgtgcact tgggaaattt gccttttctt cgttgaaatc
     3361 cctgaaatgt gctcttaatg cacctttcaa tgcagatgct tgtacatgag gccagttagt
     3421 atgtctttca cgctgaatag gtaaatcaac tgcgctaaga gcgtttccac ttcctgcatg
     3481 caaaggagat atgttttcaa attttaaaat agaaattttt tctgtcttga acatgacccc
     3541 tccttaaatc tgtatcatat tttctgaaat ctttgccgaa aaaacgcttc cggcgggata
     3601 ataccctttc attggtttat gaaaacgccg gtgcatatcc caaccacctg catatattgg
     3661 ttttccacca gcaataagta cactatttga ttgctcattc ccttcaataa gggaaagtgc
     3721 cataaaaatg tcattcctac cagaaggaat aaccggtgct ggcattgaat taactaagca
     3781 cattcgttgt tcagcaccta accttatatg gaaagaattg gctagaccaa tatctttatc
     3841 aattccacat acaattttaa cacctttttt taatctagca tgtacaaaag aatataaatg
     3901 accacttcgc acactttttt gttcattaag agcaattcct gtacgccttt ctttaacaaa
     3961 aaaatgttct attggatata aacttacttt atcttttcca aagtcttctg aggataccca
     4021 cattccacct gctgtgataa gatcttgcac accacttttc acccagtgaa tttgactttg
     4081 tccgttaact attacaagat cattagataa ctcatataaa ttttttatta ttacttttac
     4141 atttggatat atatcactta tatttgcttt ctccatagaa tgcttttcta caaaccatgt
     4201 atatggtgcg ggtaaaaaca gcatttcatt catggtgaag aggggaccaa ttacagtgaa
     4261 tggtgcagca gatcccgatt gtccaatttt tttcaaaagg tcttcaggtg ctttcccttc
     4321 attataatca gcaaaagaaa ctccagtagc ttcaatacat gcagttctca atgcacccga
     4381 tattgtttca gtatgaggag ggaaaataag cgttgcacta tggtcggatc ccatcaccat
     4441 gggctctgca cccctgaaga ataatgtatc aagaggttca attgaaaacc atttcattgt
     4501 tcaccccccg ctaaaaaatt tgctattatt aacccttcag gaacaaattt tttattcata
     4561 tacgtagttt gtacaatcat agatgaaata ttttcaatat tagggtcttg ttcagaaaaa
     4621 agcgattttg ttaaaagttt ttgcacaaat tttcgataca gctgtaatga ttcatctttt
     4681 tcaataagtg gtattatacc atcacgcatc tcatccaatc tatatataag tgaagttgaa
     4741 aggcgttcct ttgttccttt tgttcctaaa agcattataa gattttgaaa actttcccac
     4801 actaaattgt cccattttgc tgcaaaatac cgtgatccgc cagatcgctt tttcaactca
     4861 attgcacaag catttcttcc acacatttct tttgcatata attcaagcag ggaatgggca
     4921 cgtgatatca tttctgaaag actttcttta tgatgacata taagtattgc tgcagaaatt
     4981 gaaatttttt cacctttgcc aagatgcata gttatctttc ctggttgtgg ttcccaattt
     5041 ttttcaacaa tagcaggctt acccatatca tctataaata caaatggttg agaatacatt
     5101 ttttgtattt cacgtgctgc aggtaacgca ttaaatgctg gtaataccgc acatacatca
     5161 tcaccacctg cataaataag cctaccacta aattgttcaa taatattctt tacactaaat
     5221 agagaaaagt cgctaagagc ttcagatata gtagcatgaa gtgcaggcga caacattctt
     5281 ttctttatat tactatcatt aaaaatttca ttccatattt ttacataacg ttcttcaaaa
     5341 gtttttcttt gtattttttc aatcacttct ggatgtaaaa cagtctccca tcttgcagct
     5401 acactttctc cattaataag tgaacccata ttatccccat ccattaaaag gatagcaaga
     5461 tatttatcct cttctcgcaa tttttgtttt aattcttttt taatctcacg ttcaaattca
     5521 tccttttgat ggagttgctg agcatacaac caacgtttgt ttttatcatc aataccgcaa
     5581 cgcttgaaat aaccattgag agccatttct gtagtggaag ggaaagcatc agcattttca
     5641 aacacacctg aaagaaggtg ttgcttttct tttgtaacct gtgcagcaag tcgcttaact
     5701 aaacacactg cacatagttt ttccccttcg ttaaaatcat attcactata agcattcttc
     5761 agattatccc aaaatatttt tattttatga acatattgtt ttgcagttaa attttctgga
     5821 tgagcaatag tatgaagtac ttcaaattgt ccacaaagat gacatttttc accattttcg
     5881 ttatttcgcc taatctttct tttaattttg gcagacgcta aagcactctg agcaagtctg
     5941 tggctaaccg tataaaaaca tccctgtgaa gatccttcaa ccaatgtgtt gaaaagtttt
     6001 gaaagcttat tatgagatga ataaacctct tcttcaaaaa atttattaag tttagcgata
     6061 tctctttcac ctactagttt agtgcaggca aaatcaatat cccagaaatg ttctgtttgt
     6121 cttttaaata ttttaaaaaa tactttttta tcttcaactt cctttataca acgctcagca
     6181 actgaatttt gtataagatc gcacaattcg ttccaacgca taataactgt ttgtttaata
     6241 gcctcacaaa attcatttgc ctttccaaat ggcaatacaa ataaaaattt gttaggtaat
     6301 gttgcaattt ccaatggttt tggaagacaa gattcttgaa taagtgaatc aaatcctggt
     6361 atttttttta aatacaattc aaccagtggc tggtcaagaa gtgatggata taatacatga
     6421 tcaggcccta attgttcaat aacatatcgg ataccttcaa atgcaagcca tgaaagcaac
     6481 accgatgaag tccagtaatc acgaagtttg cgtgcttttg caataaatcc ctgcactggc
     6541 gttatactaa aaacagctaa tccaatgcct tcttcagctt tagccaattc cattgaagaa
     6601 taaagtgatg aaacaagtgc attatgatgc catatagaat gatcaggaat tctggtatca
     6661 gcaggcaatc tatgccataa cgcaccaagt ccaccaatat tttttgatga gagtctgtaa
     6721 cgcagtaaaa aataaacata aaaaaatcgg tttctcgctt ttttaatttc atctccctga
     6781 gaaattacct gcatatcact ttcgattaat ttaataattt cttcctgatt tacactatca
     6841 acagctaaca aaacatcaag tttatcattg cttgaaaccg gatgagttat aattgcatgc
     6901 tttaaaaaat ctactgctcc acttttttgt acatcagcct gatatcctgg gaatacacct
     6961 cgttcaaacc cagctgccaa tatatctgcc attttccaaa atttatcttc tggtggctta
     7021 agcccaaacg cttccaggta ttgagatgct ctcttttcat ggccaggtat attaagtgct
     7081 ttatcaaatg gatcatggaa aaaggctgct aatttatcat tccaatataa tgcatttgtt
     7141 tccaccattt cctccttaat aaattgtagt cattttttca tttttttgta ttttttcaaa
     7201 gatgtatatc atctttttaa tcgtattatc atattcgtta tttctattgt taagaggctt
     7261 actataatta tatggcaata tgagtatttg tcctttatat cctttttcat tttttgatac
     7321 atgtaaaaag catggtttag catgtcttcc ttttttcact tcttcattat ttgcctgtac
     7381 aataggcagg ccaatatact tacggttgcg gtaatcatgt tttctatcca tttctgttga
     7441 aagacgcatt tgtcggtagg caatacctat atctgaaagg gcatctatcc agtttttttg
     7501 ctcattaaac aaaaatatct tggattttga tgaaaaggtt gtataaggta gtacatttac
     7561 attcttattt ttatcaagga aatttatata aggaacatct tttgaaaata aagaaccaaa
     7621 accatttcta ctttttgctc ccaaaccacc ataatgaata agtgcactta aagctttttc
     7681 tatttcatct tttatactct cagaatcata tgtacaatga ataataaaag tactccccgg
     7741 tgcaatgttc tttcttgccc attgccttgt tttaggatca taaataccta taccaagata
     7801 tgtaaaaata tcaatattaa attgcttctt agaagaatta actgcaacca tcttgccttt
     7861 aggcatattt tgctcaattt ttacttcttt tggttctatc attaaagaaa atgacgattt
     7921 acaagaactg tcattcatag tagaaccaaa tattttacct tctttatttt gtgaattatt
     7981 caaatcatta attggattta ttgctctcca ccagaaacgt aacaatcctt ttaatgaaga
     8041 cgctctaagt tctgaaattt gagtatcagc acctgaaata aaaagtggag taacaacttc
     8101 aattaaatag ctcctcttta ttcgaccaca atgtttttcg atatagaagg ccatttgtcc
     8161 tccttatgtt aacaaaagtt cattaaatat attaggataa cccacattat gcgattcaaa
     8221 tatagatgca tacgggcaat gaagaataaa ctga
//
LOCUS       DAORYL010000104         8252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_6257_length_8252_cov_13.463828, whole genome shotgun sequence.
ACCESSION   DAORYL010000104 DAORYL010000000
VERSION     DAORYL010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8252
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6257_length_8252_cov_13.463828"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1355)
                     /locus_tag="PLV81_13765"
     CDS             complement(<1..1355)
                     /locus_tag="PLV81_13765"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOM11205.1"
                     /translation="MVKTIKTIIILTILIIPLFAPAHSLITLPHIETIDSNGVPVFYA
                     QDTLPQCTVYVSIGVGSLYEHATNAGISQMAALVLSLGGSKNYPNINEYIDATGGRYT
                     VSASFETLTISMRVHQDEAAQAFTILADIIANPLFETQLMQTARFMLKQQIQQKQDDP
                     MTIAMEKARQYVFGDSGYGAVMTPESLDAITVADVEQLWKKYVVKENVRIGIVTSLIK
                     GNVMQQVAQLTDAIPHGEAVDYPVDMQNAKNTISTKKIYFIEKDIPQSTIVIATVAPS
                     IKSNNRYALEAGNYILGGGSFNSWLMDEIRVKRGYAYSTGSIVRMRKNTGLFLAYAQT
                     SAATTLSTIEIMENVLANFAKTGPSQQQLQWAKDALLKSYIFEFTSLRDIVQYYMWLD
                     YNKLPVSYIQKYPDGITSVTNNDITRSFESIASGYIIVVVGNKDVKQQLDAGKINYSV
                     A"
     gene            complement(1496..2986)
                     /locus_tag="PLV81_13770"
     CDS             complement(1496..2986)
                     /locus_tag="PLV81_13770"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOM11206.1"
                     /translation="MNIHQRLIVIAFFIITLLPMNYRPCFANPLLENINVEQHTLPNG
                     ITCLLVNRGYTPTLALIISFKVGSVDEHYQTAGAAHLLEHMMFKGTKTIGTTNFQEEQ
                     KLLQQIEALGETIDKISLTNPENVQLSQLKQRLQTLQEKANAYVVNSAYDAIYTQAGG
                     INFNASTSRDMTQYYIELPNDALELWAKLESERIKEPVFRQFYTERNTVYEERLMRYE
                     SDPQSKLFEDFLGIAFLAHPYRHPTIGYTSNIPFLSLSLVRKFYFDHYVPQKMTITIV
                     GKQDTKQTLKILKQYFGTIPQASAQDFVAIQEDSKNTRRLVTYADATPYLLIGWHKPT
                     MPHFDDYVFDVIAEILAGGKSSRLYKALVLDKKIVSQVDAYNGYPGSRYNNLFILEAT
                     PKDSSQCEAVEELLYRELEALGTTCTQQEIDAAIRRLESSLIFDIDTNLGIARLVSYY
                     QTITGNWHYIVTYSDRLRTIRCADIKRVLDTYCKKEWATVAVLKKR"
     gene            3144..3476
                     /locus_tag="PLV81_13775"
     CDS             3144..3476
                     /locus_tag="PLV81_13775"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOM11207.1"
                     /translation="MKRIVLHNDTSHQLTKTKLINFLQSNKEYFKYKYGVMRIGLFGS
                     FAKNKYTSTSDIDLIIMMENEKKNIHNYLAFKKELEHVLKRKVDIGFEHTIKKSVKDE
                     ILKDIIYV"
     gene            3469..3813
                     /locus_tag="PLV81_13780"
     CDS             3469..3813
                     /locus_tag="PLV81_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012057895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOM11208.1"
                     /translation="MYNKKILLYLNDIKDSINAINNYITSMTFDEFINDRKTYMAVIR
                     EFEIIGEAVKNIPDNIKTKYPDIQWRDIVDFRNLLIHQYFGVDLEIVWRTIHEDLPPL
                     KNTIEDIEKNVS"
     gene            complement(3891..5246)
                     /locus_tag="PLV81_13785"
     CDS             complement(3891..5246)
                     /locus_tag="PLV81_13785"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HOM11209.1"
                     /translation="MKKILILFLAIVTVNLCTFLYAQEEYIPLPDVAGDPDLATAEKP
                     VQPFTLRYGGWITPVVIDQRTTDTDLMTSITTARVWLSSTLWANASLYVRVKDSFLAV
                     IREQDVNIDNTSNVFDLDIAQVTIPVMKNKMRLVLGRKYYVTGAGLALNGRGDGGEVQ
                     LYSKYGDLKAFGMYSGLLNKDDNPYGLSSRDIADGAKRIFAGAQYSKDFLFNNTVYLY
                     GLMEIDKQDEEANNKSTYDAQYIGAGVKGVVSDSLSYYAEYVYQMGTSYATIVPGVQE
                     ESDIKAMAVLAGLRYFIKAAYRPLINLQYAYGSGDEDATSYSNATGNTLDDDTRFMYF
                     GTFLGGFALRPQLGNLHVIRPGIGFSPFYNGGDRIKALNLVLYYTYYMKDKKESPISG
                     GDAPLPESFVGQGVDLMLRWPVYYDFSFFANYGLFLPGDAYPSGTPERHFIMAGVNIV
                     F"
     gene            complement(5243..5665)
                     /locus_tag="PLV81_13790"
     CDS             complement(5243..5665)
                     /locus_tag="PLV81_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11210.1"
                     /translation="MRTLTILLLTLCMANATFAQSATLALKVAQKPQATVEDAMILYL
                     QVTKGITQIKTEELISQGLLPRGVKSETPLTKGILAYMIAKQEHITQSLMFNIFKSKR
                     YAVFACIAAGYMPQGSGELDPVSGVELLEVLGRVGGEE"
     gene            complement(5979..6716)
                     /locus_tag="PLV81_13795"
     CDS             complement(5979..6716)
                     /locus_tag="PLV81_13795"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOM11211.1"
                     /translation="MKHMHTLLIIAITLLFAASPLFAAITVVSVKGEALYSDGAAFKA
                     LQKGMQLKEGTKIQTGAKSKVVLDIDGHIVTIKSFTLIKVYKNIAKKDESTNSLGLAY
                     GSINAKVKKLPQVKTSFLITTPVATSSVRGTEQNVSFGPGFGMQVTVPEGELNVLSNG
                     ENKKVGGNLEYSKGTDDPRSGDLTQGMQNTYIGQMAPGNLTPEEKDMLNQYGDQLVDS
                     TQTPADVLDNTGGAATVNMNLIWNVQP"
     gene            complement(6849..7463)
                     /locus_tag="PLV81_13800"
     CDS             complement(6849..7463)
                     /locus_tag="PLV81_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11212.1"
                     /translation="MSLFDRVLAFFSLSQPLQADPVPAYVNFEEMMVNVTAPDIDTIS
                     LHFSYTEIVNNNGRITLEVPAGSQRTFEVVAGNGGESRVYGGITTVDLVPGQQVNLNI
                     EMGQLAYTSPTFNYMSNNVEIIYYKNNNISAPIFFKVYHNIGVAQPIYELIDTVAVSS
                     LGYDEDNYSITYHYSNSEINSDNRMLYYISALNKYGEGEKVQIY"
     gene            complement(7631..>8252)
                     /locus_tag="PLV81_13805"
     CDS             complement(7631..>8252)
                     /locus_tag="PLV81_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11213.1"
                     /translation="IRQQAQLSLFDRVLAFFSLSQPLQADPVLGNLPYPVNLITVNVS
                     AHDMVTITREIPLETGQITLEVPAGSQRTFEVVAGELAVGAPVFSRYYGGITTLDLSP
                     GQQVNLNIEMGKLVIVNEWDRSFDQNNSLFIYINCNSPSSIIGFNIYLKINEQLILQD
                     TVTEFDNPSSGYYQFYYPSFNEEYVNNYYLSGINKYGEGNSVQRPQ"
BASE COUNT         2473 a         1716 c         1470 g         2593 t
ORIGIN      
        1 ttagcaacac tatagtttat tttaccagca tccaattgct gtttaacatc tttgttaccc
       61 acaaccacaa taatatagcc actggcaata ctttcaaagg aacgggtaat atcattattt
      121 gttacagatg taatgccgtc aggatatttc tgaatatagg atactggtag cttattgtaa
      181 tcaagccaca tgtagtactg tacaatatca cgcaacgatg taaactcaaa gatatagctt
      241 ttcagcaatg catcttttgc ccactgcaac tgctgctgtg atgggcctgt ttttgcaaaa
      301 ttagccaaaa cattttccat aatttcgata gttgacagtg tcgttgctgc tgaagtctgt
      361 gcgtaggcta aaaataatcc agtatttttc cgcatgcgta caattgatcc ggtggaatac
      421 gcatatcctc gtttcacccg tatttcatcc atgagccatg aattaaacga accaccgccc
      481 agtatgtaat taccagcttc cagtgcatag cggttgttgc ttttaattga tggagcaacc
      541 gttgcaatga caatcgttga ctgcggtata tccttttcaa taaaatatat tttttttgtg
      601 ctgatagtat tctttgcatt ttgcatatcg acagggtaat caacagcctc accgtgaggt
      661 atagcatccg tcaattgagc tacctgctgc atgacgtttc ctttaatcag cgacgttaca
      721 atgccaatac gcacattttc ctttacaacg tatttcttcc acagctgctc tacatcagca
      781 acggtaattg catccagcga ttcaggggtc ataactgctc catagccact atcgccaaat
      841 acatattgcc gtgctttttc catggcaata gtcattggat catcctgctt ttgctgtatc
      901 tgctgcttca gcatgaagcg cgctgtttgc attaactgtg tttcaaaaag cggattggct
      961 atgatgtcag caaggattgt aaaagcctgt gcagcctcat cctggtggac acgcatgctg
     1021 atggttagcg tttcaaacga ggcactgact gtatagcgcc cccccgttgc gtcaatatat
     1081 tcatttatgt tgggataatt ctttgaacca ccaagcgata gcaccagcgc tgccatctgg
     1141 ctaataccgg cattggttgc atgttcatat aacgagccaa ctccaataga aacatacact
     1201 gtgcactggg gcaatgtatc ctgtgcataa aataccggaa caccattgct atcaatggtt
     1261 tcaatatgtg gcagtgttat aaggctatgt gctggagcaa acagtggtat gataagtatt
     1321 gtcagtatga ttattgtttt tattgtcttt accattatgc attctccgtt gaaaatgtta
     1381 tctattacgt acagccaccc tgagtactgg tgaaatcaat tgattgttaa aagaataaga
     1441 ttcttccccc aatcacatcg gggtctgaat gacgcaataa cagataccaa catcctcacc
     1501 gttttttcaa cactgcaacg gtagcccatt cctttttgca ataggtatca agtacacgct
     1561 tgatgtcagc acaccttatt gtgcgcaacc tgtcgctata ggtcacaata tagtgccagt
     1621 tgccggtaat tgtctggtaa taacttacaa ggcgcgctat tcccagattg gtatcaatat
     1681 caaagatcag actggattca aggcggcgta tggctgcatc aatttcctgc tgagtacatg
     1741 tggtaccaag cgcctcaagt tcgcggtata aaagctcctc caccgcttca cactgtgaag
     1801 aatcttttgg cgttgcctca agtataaaaa gattattata ccgtgaacct ggatacccgt
     1861 tatacgcgtc aacctgcgaa acaatctttt tatcaagtac cagtgcctta taaagccgtg
     1921 agcttttgcc accggcaagt atttcagcaa taacatcaaa tacatagtca tcaaagtgtg
     1981 gcatggtggg tttatgccag ccaattaaaa gatacggcgt tgcatctgca tatgttacaa
     2041 gccttcttgt gtttttacta tcttcttgta tagcaacaaa atcctgtgct gatgcctgtg
     2101 gtattgtgcc aaaatactgt ttcagtattt tcagcgtctg ttttgtatcc tgcttaccca
     2161 caatggtaat ggtcatcttt tgcggtacat aatggtcaaa ataaaatttg cgcaccaggc
     2221 tcaatgacaa aaacggaata tttgatgtat aaccaatggt gggatgccgg taaggatgtg
     2281 caagaaatgc aatacctaaa aaatcctcaa acaatttact ctgtgggtcg gattcatagc
     2341 gcataaggcg ttcctcatac acggtgttgc gttcggtata aaattgtctg aatactggtt
     2401 ccttaatgcg ctctgattct aattttgccc acagttcaag tgcatcgttg ggaagctcaa
     2461 tgtaatactg cgtcatgtcg cgtgatgtgc ttgcattaaa gttgatgccg ccagcctgcg
     2521 tgtagatggc atcatacgct gagtttacca catacgcatt tgccttttcc tgcaatgttt
     2581 gcagacgctg tttcaactgc gacaattgca cattttctgg atttgttaat gaaattttat
     2641 ctattgtttc acccagtgct tcaatctgct gcagcagttt ttgttcttct tgaaagtttg
     2701 tggtgccaat tgtttttgta cctttgaaca tcatgtgttc aagcagatgt gcagcacctg
     2761 cagtttgata atgttcatca accgaaccca ccttaaacga aatgattaat gcaagcgttg
     2821 gtgtgtatcc acggtttacc agtaaacacg ttatgccatt aggcagtgtg tgctgctcaa
     2881 cattaatatt ttccagcaac ggatttgcaa aacatgggcg atagttcatg gggagcagtg
     2941 taattatgaa aaaagctata actattagtc tttgatgtat gttcatagat tataatttct
     3001 cctgtttata aatttcattt tataaatatt tacatacatt tcaaatattt atcttgacac
     3061 tcatttttta tttttgtttc ttaataattg ataaatgttg caaaatatta ctaaaaaatg
     3121 aataaaagct tatgagtaat caaatgaaac gcattgtatt gcataacgat acatcgcatc
     3181 aattaacaaa aactaaactt attaattttt tacaatcaaa taaagaatac ttcaagtata
     3241 aatacggtgt aatgagaatt ggattatttg ggagctttgc taaaaataaa tatacttcta
     3301 ccagtgatat tgatttaata attatgatgg aaaatgaaaa aaagaatata cacaactatc
     3361 tagcctttaa aaaagaatta gaacatgttt taaaacgcaa agttgacata ggttttgaac
     3421 acactataaa aaaaagtgtt aaagatgaaa tactaaaaga tataatttat gtataacaaa
     3481 aagatattgc tatatctcaa tgatatcaaa gactctatta acgctattaa taattacatt
     3541 acatctatga catttgatga atttatcaac gatagaaaaa catacatggc tgtaatcaga
     3601 gaatttgaaa tcattggcga agcggttaaa aacataccgg ataacattaa aacaaaatat
     3661 ccagatattc aatggcgcga tattgttgat tttcgcaatc ttttaattca ccaatatttt
     3721 ggtgttgatt tagaaatagt atggcgaacc attcatgaag atttaccacc tttaaaaaat
     3781 actattgaag atatagaaaa aaatgtatca taaaagtaaa aacgccatgc atgacgtttg
     3841 tacgatgcat ggcgttttag catttatatg attaaaactc tattatcaaa ttaaaataca
     3901 atatttacac cggccatgat gaaatggcgc tctggtgtac cggatggata tgcatcaccc
     3961 ggtaaaaata atccataatt tgcaaagaat gaaaaatcat aatacactgg ccagcgcaac
     4021 atgaggtcaa caccctgacc tacaaatgat tctggtaaag gagcatcccc accgctgata
     4081 ggagattcct ttttatcctt catgtaatat gtgtaatata aaaccaggtt cagtgccttt
     4141 atacggtcac caccattata gaatggcgaa aatccaatac ccggcctgat tacatgcagg
     4201 ttgccaagtt gcggacgtag tgcaaaacct cccagaaatg taccaaaata catgaagcga
     4261 gtatcatcat caagagtatt gcccgtagca ttgctatagc ttgttgcatc ctcatcccca
     4321 gaaccatacg catactgtag attaattaac ggacggtagg ctgcctttat aaaatatcgc
     4381 aatcccgcca gcactgccat tgccttgata tcactttctt cttgaacacc aggtacaatt
     4441 gttgcatagg atgtgcccat ctggtaaaca tattcagcat agtatgacag tgaatcgctt
     4501 accacaccct taacaccagc accaatgtac tgtgcatcgt aggttgattt attattagct
     4561 tcctcatcct gtttgtctat ttccatcagg ccatacaggt aaacggtatt attaaacaga
     4621 aaatcctttg aatactgtgc acctgcaaag atacgctttg cgccatcagc tatatcgcga
     4681 gaactcaacc catacgggtt atcatcttta ttaagcaaac ctgagtacat gccaaatgct
     4741 ttcaggtcgc catattttga atacagctgc acttcaccac catcacccct gccgttgagc
     4801 gcaagccctg cgccggtaac atagtacttg cggcctaaaa ccaaacgcat cttatttttc
     4861 atgaccggga ttgtaacttg tgctatatct aagtcgaata cgttgctggt attatcaata
     4921 ttaacatcct gctcgcggat tactgctaaa aagctatcct taacgcgcac atacaggctt
     4981 gcatttgccc acaatgtgct ggacagccac actcgggctg ttgtaataga tgtcatcaaa
     5041 tcggtgtcgg ttgtacgctg gtctataacc accggcgtaa tccatccacc ataccgtaat
     5101 gtaaacggct gcacgggttt ttctgcagta gctaaatcgg ggtcaccggc aacatcaggc
     5161 agtggaatgt attcctcctg tgcatagagg aaggtgcata aattaacagt aactatcgcc
     5221 aaaaaaagta ttagtatttt tttcattctt cacctcccac tcgtcccaga acttcaagca
     5281 attcaacccc acttaccgga tcaagttcac ccgaaccctg tggcatatac cctgctgcaa
     5341 tacaagcaaa aacagcatag cgcttgcttt taaagatatt aaacataagt gattgggtaa
     5401 tatgttcctg ttttgcaatc atgtatgcaa gtataccttt ggttagagga gtttcagatt
     5461 tcacaccacg cggtaatagt ccctgagata ttaactcttc cgttttaatt tgcgttattc
     5521 ctttagtaac ctgcaaatac aatatcattg catcctcaac cgttgcctgt ggtttttgag
     5581 ccactttaag agctaatgtt gcactctggg caaaggttgc atttgccata cacagtgtta
     5641 aaagtaatat ggtaagtgtt cgcatgtatc ctcctttgta ctggttattg gtatatttca
     5701 atgaaaataa aattttcttt tctatttaaa tcaatctcac ttttgatttt aacaagaact
     5761 gattggatac cataattttc agcaatgata atacgtttgc aatgggggct gtccctaaag
     5821 aggtggttga gtaaattttg caaacattat atcgtaatac tcgctcattt cgatacaatt
     5881 ttgccacaaa atctggcaaa attactcaat gagcgttgcg aaatttgtat cgaaacgact
     5941 attgggacaa tccccaaaga gtatgttcca taaaattact acggctgcac attccatatt
     6001 aaattcatat ttactgttgc ggcgcctcca gtgttatcca gaacatctgc cggtgtttgt
     6061 gtggaatcta caagctgatc gccatactgg tttagcatat ccttttcctc aggtgtcagg
     6121 ttacccggtg ccatctggcc tatgtaggta ttctgcatcc cctgggtaag gtcgccactg
     6181 cggggatcat cggttccctt gctgtactcc aggttgccac caaccttttt attttcgcca
     6241 ttagacagca cgttaagctc accttcagga acggttacct gcatgccaaa cccaggccca
     6301 aaacttacat tttgctcggt accgcgtacg gatgacgttg caaccggtgt ggtaattaaa
     6361 aagcttgttt ttacctgtgg cagcttttta actttggcat taatgctgcc atacgccagc
     6421 ccaagactgt tggtggactc atcttttttg gcaatgtttt tgtacacctt gataagggta
     6481 aatgatttta tagtcactat gtgaccatca atatctagca ctacttttga ctttgcacca
     6541 gtctggatct ttgtgccttc tttaagctgc atgccctttt gcagtgcctt aaacgcagct
     6601 ccatcactgt acagtgcctc ccccttaacg cttaccaccg taatggccgc aaacaagggg
     6661 cttgctgcaa agagcagtgt gatggcaata atgagcagtg tatgcatatg tttcattggg
     6721 tttcctcctt aaaatataat ataattatgt aaaatatata taatcaattt ataaaataac
     6781 tttttaataa ccaataaaac cttatttagg ttaagttatt agttaaactt ttatttaaat
     6841 ttaaatattt aataaatttg cactttttcc ccttcaccat atttatttag tgcacttatg
     6901 tagtacaaca tcctattatc actatttatt tcactattgc tgtaatgata agtaattgaa
     6961 taattatcct catcgtaacc cagggagcta acagcaacag tatctatcaa ttcatatatt
     7021 ggttgtgcca caccaatatt atgatatact ttaaaaaata ttggtgcaga aatattatta
     7081 tttttgtaat aaataatttc gacattgttt gacatataat tgaatgttgg actagtataa
     7141 gccaactgcc ccatttcaat attcagattc acctgctgcc cagggacaag gtcaaccgtg
     7201 gttatcccac cgtatactct agattcgcca ccattacccg caacaacttc aaatgtgcgc
     7261 tggctgcctg cgggcacttc taatgtgatt ctgccattat tattaacaat ttcagtataa
     7321 ctaaaatgta gcgaaattgt atcaatatct ggagcagtta cattgaccat catctcctca
     7381 aaatttacat aagccggtac aggatccgcc tgcaatggct gggacaggct aaaaaatgca
     7441 agcactctat caaataagct caattgcgcc tgctgacgta tcccagtatc aatggttact
     7501 gtggttgtgt tttcatagct gcagcttatt gtaagtagaa caactatgaa tgaacaatat
     7561 gcaattactc ttttcatatc ttcaacttcc cctccccaaa tattgtaaaa taaattgtat
     7621 tttcataatt ttattgtggt ctttgaacac tattaccttc tccatattta ttaataccag
     7681 ataaataata attatttaca tattcttcat taaagctggg gtaatagaat tgataatatc
     7741 ctgaacttgg attatcaaat tcagttaccg tatcttgtaa aattaactgt tcatttattt
     7801 ttaaataaat attgaatcca ataattgaac ttggtgaatt gcaatttata tagataaaaa
     7861 ggctattatt ctgatcaaaa ctacgatccc attcattcac aataacaagc ttccccattt
     7921 caatattcaa attcacctgc tgcccaggtg aaaggtcaag ggttgttata ccaccataat
     7981 atcttgaaaa aactggagct ccaactgcta actcaccagc tactacctca aatgttcgct
     8041 gacttccagc tggcacttca agagttattt gtccagtttc aaggggtatt tctctggtaa
     8101 ttgttaccat atcatgagca gatacattaa cggtaattaa attaactgga taaggcagat
     8161 tacctaatac tggatccgcc tgcaatggct gggacaggct aaaaaatgca agcactctat
     8221 caaataagct caattgcgcc tgctgacgta tc
//
LOCUS       DAORYL010000105         8233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_6279_length_8233_cov_9.295427, whole genome shotgun sequence.
ACCESSION   DAORYL010000105 DAORYL010000000
VERSION     DAORYL010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8233
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6279_length_8233_cov_9.295427"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1921)
                     /locus_tag="PLV81_13810"
     CDS             complement(<1..>1921)
                     /locus_tag="PLV81_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11214.1"
                     /translation="DQGILIATIQAGSSYSATLVQEQPQIIVSGIADMPDDGVATNLL
                     YDADTDFSIYAWLSNGDLLLTNVAGINWNSDTLANQGATPIDTFQYVLKSSYNANLDS
                     FDFYIADNISFSGLADSGTTTTELRSSTDANFLLVQPGDIIHNETNNEWGLASGAGTW
                     NGSYYLIPIDRTLTSLGDGDTYRIYTNHSTLLTSEAVINPLWDKDPSAPFNPGTTVLE
                     NDIVVNENNNSVAATYAYVDTIDLSRDTNYALRLTNNIMDNGDIYSIIRMPAGVTVKG
                     VGYSTGVASFTLNDTYANFTGWPVNPGDIVFNIDTSISAMVISVTGVNQLTLSADIFN
                     ATNQKYIIYTKRAFLVSYIDVGNNVMANVFNIADGSLLHSFAVCTDGINSNPHAITDE
                     AGNAIIFYEKAGTIYAKKVTATGQFFASWGVSADEASDPGRSILTGYSIVQVAPVKNT
                     GGAGDVYLLAKNSAGTQFRLLRINSNDGTTSFDSTPITGSDPIMAIDSVTGQINRAII
                     VYRNTHVTGGITYYHIEARAYGGTSWGPIIISSNAADYNCVQPVITMANNTTAADNFY
                     VSWFDGRHYGTNGYSIYMQRYNATPAAQWAANGIFISFPTSFGYDYPLLLKLLYYYDG
                     GSPYGALPLWLDYREY"
     assembly_gap    1922..2021
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2022..3245)
                     /locus_tag="PLV81_13815"
     CDS             complement(<2022..3245)
                     /locus_tag="PLV81_13815"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOM11215.1"
                     /translation="MKTITMNRLIKNRGDSYLVAVLLCVIIPFAVHCGAEAPSVTPNP
                     LYQSVNPTDPGTLSPQVIGIYPDDGSTDIPVDTSIVIVFNKPIDISTINPSTINILSV
                     TSFTVTPAQDNRAIILDITNPPQFGYNQTIDITVTPSVRDIDGNPLLTNYTSQFTTGI
                     NDSSYFLPKIVAISRFPQPGATGVSRDIGFVEVTFTKDMNDSTITVVNFGFSGPATSV
                     TKINPKTYRLNINTLNYNTTYSITLSGSIEDSDGNPLDLDGNHTWQFTTELDPATIPL
                     SITALWISSVTSDSITIQFTTSKPVPKNQCYAVYDTNPTVTTADTHVQEDSSSDVTTL
                     HTVTISSLSSRSLYYIRAGIDTSGGPPVEILSTQELSVYTNTDAANNSVLTNEVNDQN
                     GLVVLQTNGPASYVFW"
     gene            complement(3325..5691)
                     /locus_tag="PLV81_13820"
     CDS             complement(3325..5691)
                     /locus_tag="PLV81_13820"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOM11216.1"
                     /translation="MKINKKRIFLIILLLALTFNRAHSTIAFIILTPDNPTTLSNGLS
                     YYLAGITYNFSVNVADPDISGWAQLTDVRLTITNTTNIVIFINPSGTGTDLPVTVSSG
                     TVNAVADVSGTYNNCTVTFKVTFRWDTPESPFGAKNITGQATTTFPANNTKTDTKSVN
                     YGVCSSFQILNFAHNGVAADGMINPWHDSFTISGVPVYNVPGATLADAIGSIDSGEIT
                     NTLLYRSGNNTGYSDNTASDLSYTIPDQYFSSLPLGNHTWRIYASMQTAPGFEFSNNT
                     LTINCDEVEVTSIEFINGGGISNPPLNYYYRSVTQPGTQVRITARMRNGLGPMVGNVT
                     FILENITNPNDDATIVIAHGQTTGIANLNFPNPLPAAHTTQSNTYRIRQISGGAYGGD
                     TVNGQNVFARIQQPANPIIFWDNEDPPGSGTPFTAWIGFSPTADSLTLNWQPLLNADP
                     DRDFYSYKVYYKQTTESQYKIIDRLTSGYASLANPLTGSVTITGLIPLTNYDVYITAI
                     DVFGNEVQIADALPPVDQRIIGTLASTLRVQLTDGITSYNDDSFATFNQPNLRPVRKT
                     NLRVKVFIISAYNLPDTVNILVKPYPGTGFTSGGVLQGIEGIDYYRFSTIKTASNEWT
                     GYIQEQIPWMNQGNSIKFIIETIFNNVPTYSDYNSETETPATANPDDQPFTVSIETQA
                     QFTPWPTRILNNVIDDNNPYAYPAYYLTDDAYVTITVYDIKGRPVSKILDTAFRKGGQ
                     NIKENGWAGVNKANKKLGVGLYYIHIYAKRASDGKVILNSFQKVVIKK"
     gene            5767..6315
                     /locus_tag="PLV81_13825"
     CDS             5767..6315
                     /locus_tag="PLV81_13825"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM11217.1"
                     /translation="MNVTKKLVQNVIIACAIVTVVVAVMYFCAQYILYQDTPTEYIQQ
                     GSYKRAILHYTLKILKDPDNATLYYNRGLVYMMDKDYENAINDYEKALKLHKHVSYYQ
                     NKALAHFYLGQYDKSIEENTNGLKLYPQDGMLYYNRGRAYYMKGEILNALEDFKKAYT
                     LGIEPARGYIENIEKIKSKSNK"
     gene            complement(6325..>8233)
                     /locus_tag="PLV81_13830"
     CDS             complement(6325..>8233)
                     /locus_tag="PLV81_13830"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HOM11218.1"
                     /translation="KGLEPEINCKINGSDFSSGSTYDFGNLAVGHSKDIVITIENLSS
                     SDPLYLTGSPIVTKVSGGEFSVITQPSKVSIPPSGSTTFTIRFSPTVTGNQSAQFSIS
                     NSDSDENPYIINVQGNGTNATIQVDEVASGGTIDYGNVKCNTGGLAHTFTIRNNGSTD
                     LLLNSDNPVIISGTNKDLFILEPETPPSITIHPSTTTTFSIRFNPKTLLDTGTKTATV
                     VIQSNDPTTPLYTFTVNGTGITPHISISDGTNPIPNGGTFNFPDVNYGSAGISQSFTI
                     TNTGDAELLLTGTPKVQISGTGASHFSVTSQPTSPVNPLGSTTFTIQFNPTSLGTHTA
                     TVTISNDTLDANPFTFTITGNCVDIEPPVITITAPPDGSYTNGTQILSFTVSDNVGVT
                     SVQAKIGSGTFTDISSGAVINTINGWASASEGNVTIDMQAKDAANNTGTASLNLIKDI
                     TPPVINITAPLAGSPINGTQSIQFTVTDVAVATTRAYIDSGTPGNITSPAVISTINGW
                     NPPYSGTSITVYIESTDEAGNTGNTSQSFFADTIPPSGNITSPINGSPVTGTVQIQAD
                     ASDNTGGSDVDHVEFYINNNLVDTLSSSPWEYSWNTTAYTNGSYPLKIIVFDKAGNEY
                     TDDDTTVTVNN"
BASE COUNT         2449 a         1398 c         1673 g         2613 t
ORIGIN      
        1 atattcacga taatctaacc atagaggtag tgcaccataa ggagaaccac catcataata
       61 atacagcaat tttagcagga gtggataatc gtaaccaaat gatgttggaa aggatataaa
      121 aattccattt gcagcccatt gtgcagctgg cgttgcatta tacctttgca tatagatgct
      181 atatccgttg gtaccatagt gcctaccatc aaaccaagat acataaaaat tgtcggctgc
      241 agtggtattg ttagccattg tgataacagg ttgaacacag ttataatcag cggcattaga
      301 agatatgata ataggtcccc acgaagtacc gccatatgct cttgcttcaa tgtgatagta
      361 agttattcct ccagtcacat gagtattcct gtaaactata atagctctgt taatttggcc
      421 tgtaacggaa tcaatagcca taatggggtc agagccagta ataggagtac tgtcaaacga
      481 agtggtccca tcattactgt taattctcaa aagcctgaat tgtgttccag ctgaattttt
      541 ggcaagcaaa tatacatcac cagcaccacc tgtgttttta actggagcaa cctgtacaat
      601 agaatagcca gtaagtatgg aacggccagg atctgaagct tcgtcagcac taactcccca
      661 ggaggcaaag aattgtcctg tagctgttac cttttttgcg tatatagtgc ctgctttttc
      721 ataaaagatt atagcattac cagcttcatc ggttatggca tgaggattag agtttatgcc
      781 gtctgtacac acagcaaatg aatgtaacag cgatccgtca gctatattaa atacatttgc
      841 cataacgtta ttgcctacat ctatgtaact aacaagaaat gcacgtttgg tatatataat
      901 atatttttga tttgtagcgt taaatatgtc agctgaaaga gtaagttgat ttacaccagt
      961 aactgaaata accattgccg aaatactggt atcaatattg aaaacaatgt cacccggatt
     1021 aactggccaa cctgtgaaat ttgcatatgt atcatttaat gtaaaacttg caacacctgt
     1081 actgtaacca acacccttta cagtaacgcc tgcaggcatt cttataatag aatatatatc
     1141 gccattgtcc ataatattat ttgttaggcg caatgcataa ttggtatcgc gtgaaagatc
     1201 aatggtatca acataagcat acgtagcagc tacagaatta ttgttttcat taacaactat
     1261 atcgttttca agcactgtag ttcctggatt aaatggagca gaaggatctt tgtcccataa
     1321 aggattgatt acggcttcag aagttaaaag agtagaatga ttggtatata ttctatatgt
     1381 atctccatca cctaaactgg tcaaggttct atcaatagga attagatagt aactgccatt
     1441 ccatgtacct gcacctgagg ctaatcccca ttcattgtta gtttcattat gaataatatc
     1501 gcctggttgc accagcagga aatttgcgtc agtggatgag cgaagttcag ttgttgttgt
     1561 accagaatcg gctagtccac taaatgaaat attatcagct atgtaaaaat caaatgaatc
     1621 taaattagca ttataactgc ttttcagcac atactggaat gtgtcaattg gagtagcgcc
     1681 ctgatttgca agtgtatctg aattccagtt aatgcctgca acattggtta gcagcagatc
     1741 cccattgcta agccatgcat aaattgaaaa gtcagtatca gcatcataca gtagatttgt
     1801 ggcaacacca tcatcgggca tatcagctat gccgctgaca attatctgag gctgctcttg
     1861 aactaacgtt gcactgtagc ttgagcctgc ctgtatggtt gcaattagga ttccctgatc
     1921 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccagaaaac gtatgatgca
     2041 ggtccatttg tttgtaaaac aaccagtcca ttttggtcat taacttcatt tgttaataca
     2101 ctgttatttg cagcatcagt atttgtatat acgcttagct cctgtgtgga aagtatttca
     2161 acgggaggtc ctccacttgt atcaatgcca gctctgatat aataaagtga ccgtgatgat
     2221 aatgaagata ttgtaactgt atgaagcgtt gtaacatcgc tggatgaatc ttcctgcacg
     2281 tgagtatctg cagtggtaac tgttggattg gtatcataga cagcataaca ttggtttttt
     2341 gggactggtt ttgatgtggt aaactgtatt gtgatgctat cactggtaac gctacttatc
     2401 cacaatgcgg ttatgcttag tggaattgtg gcagggtcaa gctcagtggt aaattgccac
     2461 gtatggttgc catccaaatc cagaggattg ccatcactgt cttcaatgct tcctgatagt
     2521 gtaattgagt atgtggtgtt ataatttaat gtattaatgt taagtctata tgttttggga
     2581 tttatctttg ttactgatgt tgctggtcca ctgaagccaa aatttactac cgtgatagtt
     2641 gagtcattca tatctttggt gaaggtaact tcaacaaagc caatgtcgcg gctaacgcct
     2701 gttgcaccgg gttgtggaaa gcgggatata gctactattt ttggaagaaa atatgacgaa
     2761 tcattaattc cagttgtaaa ttgtgatgta tagttggtaa gcaggggatt tccatcgata
     2821 tccctgacag atggtgttac tgttatatct atagtttgat tatagccaaa ctgaggggga
     2881 tttgttatat ctaagataat tgctctgtta tcctgtgcag gtgtaacagt gaatgaagtt
     2941 accgatagta tattgatagt tgatggattt atggtagaaa tatcaatggg tttgttaaaa
     3001 acgataacaa tagaagtatc aacgggaata tcagtgcttc catcatcagg atatattcct
     3061 atcacctgag gggataatgt tcctgggtca gtggggttaa cactctggta cagtggattt
     3121 ggtgttacac tgggtgcttc agcaccacag tgtacggcaa atggtattat aacgcataaa
     3181 aggactgcta ctaagtaact atcgccccta ttttttatta aacgattcat tgttattgtt
     3241 ttcattcata atgtatgttt atataaatat acttttaaaa agaagaaaaa agcaattaat
     3301 tttattaaat atatataaaa tgtgtcattt tttaataact accttttgaa atgaattgag
     3361 gataacttta ccgtctgatg ctcgctttgc atatatatgg atatagtaca atccaacgcc
     3421 taacttttta ttggctttat taacgcctgc ccaaccattt tctttaatgt tctgcccgcc
     3481 ttttctgaaa gctgtatcaa gtatttttga tactggtcgt cccttaatgt catataccgt
     3541 aatagtaaca tacgcatcgt cagtaagata gtatgctggg taggcatagg gattgttgtc
     3601 atcaatgacg ttgtttaaaa tgcgcgttgg ccagggagta aattgggctt gagtttctat
     3661 acttacggta aatggctgat catcagggtt ggcggtagct ggcgtttcag tttctgaatt
     3721 atagtctgaa tatgtgggga cattattaaa tattgtttct attatgaatt ttatactatt
     3781 tccctgattc atccagggta tttgttcctg tatatatccc gtccattcgt tagaagcggt
     3841 tttaattgtt gaaaatctat aataatcaat accttcaatc ccctgcagta caccgcccga
     3901 tgtaaaacca gttcctggat atggtttgac aaggatattt acagtatcag gtaaattata
     3961 ggcacttatt ataaacactt ttacacggag gttggtttta cgcacaggac gtaggtttgg
     4021 ttgattgaag gttgcaaagc tatcgtcgtt ataagatgtg ataccatcag ttagttgcac
     4081 gcgaagtgtt gaagctaatg tcccaataat acgttgatct acaggtggga gcgcatcagc
     4141 aatctgtacc tcattcccaa aaacatcaat agcggtaata tagacatcat aattagtcaa
     4201 aggtattaat ccagtaatgg taacggatcc tgttagtgga tttgccagtg atgcatagcc
     4261 tgatgttagc cgatcaataa ttttgtactg gctttcggta gtttgtttat aatagacttt
     4321 gtatgagtag aaatctctat cgggatcagc attaagcaat ggttgccagt ttaaggtaag
     4381 tgaatcggct gttggactaa acccaatcca tgcagtaaaa ggtgtaccgc ttcctggagg
     4441 atcttcattg tcccaaaaaa ttattgggtt tgcaggttgc tggatgcgcg caaatacatt
     4501 ttgaccgtta actgtatctc ccccataagc tccacctgat atctgccgta tacgatatgt
     4561 gtttgattgt gtggtgtgtg cagcagggag aggattggga aagttaaggt ttgcaatacc
     4621 tgttgtttgc ccatgggcaa taacaatagt tgcatcatcg ttgggattag taatattttc
     4681 aaggataaat gtaacattgc caaccattgg tcccaggcca ttgcgcatgc gagcggtaat
     4741 gcgtacctga gtacctggtt gggttacact gcgataatag taatttaatg gtggattact
     4801 tataccacca ccattgataa attcaataga cgttacttct acttcatcgc agtttatggt
     4861 caatgtattg ttggaaaatt caaatccagg tgctgtttgc attgaagcat atatccgcca
     4921 tgtatggttg cctaatggca atgaactaaa gtattggtct ggaatggtat atgaaagatc
     4981 tgaagcagta ttatcactat aaccagtatt attcccgctg cggtataaca gtgtgttagt
     5041 aatttcacct gaatctatac ttccaattgc atcagcaaga gtggcgccag gaacattata
     5101 tacaggtact cccgaaattg tgaaactgtc gtgccacgga ttaatcatgc cgtcagcagc
     5161 aacgccattg tgtgcaaagt tcagtatctg aaatgatgag caaacaccat aattcactga
     5221 tttggtgtcg gttttggtgt tgttagctgg gaacgttgtg gtagcctgcc cagtaatgtt
     5281 ttttgcgcca aatggtgatt caggtgtatc ccatctgaat gttactttaa atgttacggt
     5341 gcagttatta tatgtccctg aaacatcggc aacagcattg accgtgccgc ttgatacagt
     5401 aacaggaagg tcagttcctg ttcctgaagg gtttatgaat ataacaatat tagtagtatt
     5461 tgtgattgtt aatctaacat cagttaattg tgcccatccg cttatatcag ggtcggcaac
     5521 gtttacgcta aaattatagg tgataccagc taagtaatag gacaatccat tggataatgt
     5581 ggttggatta tcgggagtaa gtataataaa tgctattgtt gaatgagcac ggttaaatgt
     5641 cagtgcaaga agaagtataa ttaaaaaaat tcttttttta ttgattttca tgaaatcata
     5701 ctatattagt tatttcacgg ttgtgtcaag attaatatac catacacact gcataaaagt
     5761 gcaagtatga atgttacaaa aaaactagta caaaacgtta tcatagcatg tgcgatagtt
     5821 actgttgttg ttgccgttat gtatttttgt gcacagtata tactatatca agatacaccc
     5881 actgaatata tacagcaggg aagctataaa agggcaatcc ttcactatac gctgaaaata
     5941 ctcaaagatc ctgataatgc aactctctat tataataggg gattggttta tatgatggat
     6001 aaggactatg aaaatgctat taacgattat gaaaaggcac ttaaactgca taagcatgtt
     6061 agctattatc aaaataaggc attagcccat ttttatttag ggcagtatga taaatccata
     6121 gaagaaaata ctaatgggtt aaagctgtat ccacaagacg ggatgctcta ttacaacagg
     6181 gggagagcat actatatgaa aggtgagatc ctaaatgccc ttgaagattt taaaaaggcc
     6241 tatactcttg gaattgaacc agccaggggt tatatagaaa acatagaaaa aataaaaagt
     6301 aaaagtaata aataggtatt acatttaatt atttactgta acagtagtat catcatctgt
     6361 atattcattg ccagctttat caaatactat aatttttaaa gggtaagatc catttgtata
     6421 tgcagtagta ttccatgaat attcccatgg agaacttgat aatgtatcta ctaaattatt
     6481 attaatatag aattcaacgt gatcaacatc tgatccacct gtattgtcgc ttgcatctgc
     6541 ctgtatttgg acagtaccag taacaggtga accatttatt ggtgaagtaa tattcccact
     6601 tgggggtatt gtatcagcaa agaaactttg actggtattg ccagtattgc cagcctcatc
     6661 tgttgattca atataaacag tgatagatgt tcctgagtaa ggtggattcc atccattgat
     6721 ggtacttatt actgcagggg atgtaatatt gccaggtgtt ccagagtcta tatacgcacg
     6781 agttgttgca acagcaacat cagtgacagt aaattgtatc gattgagtac cattgattgg
     6841 actgccagca agaggtgcag tgatgttgat tacaggtggt gtaatatctt taataagatt
     6901 caaagaagcg gtaccagtat tgtttgcagc atccttagcc tgcatatcaa tggtaacatt
     6961 gccttcagaa gcagaagccc atccattgat ggtatttata acagcacctg atgaaatatc
     7021 agtaaacgta cctgaaccaa ttttagcctg aacgcttgtt acaccaacat tatctgagac
     7081 agtaaaggat aatatttgag taccattggt atagctacca tcaggtggag cagtaatagt
     7141 gataacaggt ggttctatgt caacacaatt acccgtaatt gtaaatgtaa aaggattggc
     7201 atcaagtgtg tcattagata ttgttactgt agctgtgtgt gtacctaagc ttgttggatt
     7261 aaattgaatt gtaaatgtag tacttcccaa tggattgaca ggtgatgttg gttgtgatgt
     7321 gacagaaaaa tgtgaagcac ctgtacctga aatttgtact ttgggggtgc ccgttaaaag
     7381 tagttcagca tctccagtat tggttatagt aaatgattgg gatattcccg cactaccata
     7441 atttacatca ggaaaattaa atgtaccccc attgggaata ggatttgtac catcacttat
     7501 tgatatatgg ggagttatac cagtaccatt aactgtaaat gtgtaaagtg gggttgttgg
     7561 atcattgctt tgtattacca ctgtagcagt tttggtgcct gtatccagta aggtttttgg
     7621 attaaacctg atactaaatg ttgttgttgt ggatggatgt atggttatag atggtggtgt
     7681 ttcaggttca agtataaata agtctttatt ggttcctgaa attattacag gattatctga
     7741 atttaacagt aaatcagtac taccattatt gcgtatggta aatgtatgtg ctaaacctcc
     7801 tgtgttacat ttaacattgc cgtaatcaat tgtcccacca ctggctactt catcaacttg
     7861 aatggtagca tttgtaccat ttccctgaac atttataatg tatgggtttt catcgctatc
     7921 actgtttgat atactgaatt gtgctgattg attgcctgta acggttggtg aaaaccgtat
     7981 tgtgaacgtt gtagatccag atggtggaat acttactttt gatggctgag ttataactga
     8041 aaactcacca cctgaaacct ttgtaactat tggacttcct gtcaaataca gggggtcgct
     8101 tgatgaaaga ttttcaatag taataacaat atctttgcta tgtcccactg caagattgcc
     8161 aaagtcatac gtgctgccac tgctaaaatc agagccattt attttgcaat taatttcagg
     8221 ttcaagacct tta
//
LOCUS       DAORYL010000106         8213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_6299_length_8213_cov_15.032974, whole genome shotgun sequence.
ACCESSION   DAORYL010000106 DAORYL010000000
VERSION     DAORYL010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8213
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6299_length_8213_cov_15.032974"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..127)
                     /locus_tag="PLV81_13835"
                     /pseudo
     CDS             complement(1..127)
                     /locus_tag="PLV81_13835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(206..877)
                     /locus_tag="PLV81_13840"
     CDS             complement(206..877)
                     /locus_tag="PLV81_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11219.1"
                     /translation="MEKSILTLSIILHLLFLSVLLYGDQMNIQKPPLNEKPIIKIFDT
                     EIEMPFGYYALVKKDNKLCAVRFLETGQHHKKDKRYAIYEYYYQGDGTGDFLIKTAKH
                     GMGKATYNSFCIIGRLCFNTGNMNMACGEINIKWYGGRGISYGDGLNRNEKISDVIKA
                     GVQIAPTKWTDIKDVNITEKGLKWYKYGTLPDYPEYQRFKVIENISCPPWKRFHYEIP
                     AEELW"
     gene            complement(890..3664)
                     /locus_tag="PLV81_13845"
     CDS             complement(890..3664)
                     /locus_tag="PLV81_13845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOM11220.1"
                     /translation="METGRAAIEVQYAGAIVTAEGITAPARAVTYNRSGGRYNETIAI
                     VRMADGLGRELQVKTQAQVEGKYGFTVSGARVYDAQGRVVAEGKPAFEEGNSFEYSPV
                     ALKYPTTYQYDCMGRKIEATYPDGGIVVAAYGTRDGKLIECITDQNGNSKLITRDVRG
                     NIVAVTEEGGITTTYRYDVVNQLVTVVDAAGNVTSITYDTWGRRTSIDNPDTGKTIYQ
                     YDDAGNLIRKQTANLRKQNKWINYVYNYKQVKMIDNPDSEDVYYEYGRPGDGKNGAGR
                     VVQEKTGKLVDSYWYGALGEVITKERSIDGKTYRIQWKWDNFGRVRSIIYSNNFSVYY
                     AYDAGGQVKGVVGYLGAIRTDYVKDIQYDEYGSRTRVEYGNGVVSTYEYDDRMHRLVQ
                     LQTKQGDSTLQNIAYSYDRVGNILTRTENGIQMSDRTPKTITHRYSYDSLYRLVEAEG
                     TLQENGNVVNNYRNAFTYSEIGNILSKLQIVKAQGNEDPTLTYTYTYTYAGNKPHAVT
                     GINDNLTYRYDANGNMTAVYDTAKNYNRILYWDDDNRLTKTVDTQAGVSTATQYAYDA
                     KGMRIVKDGPYGKSIYVDTGYVLSQEYVESNHIFVGNTRVASVVKHRDETKAATYYYA
                     TDHLGSSSVLTNNIGAYHERIEYLPYGEVWVEDTVNSNGYTTPYKFTGKELDKETGLY
                     YFGARYYDARISRWISTDPALQEGKYFPKLNNYDTEHDFYWYLQQDGSRKLAGMGGVF
                     NAINMDVYHYAGNNPVQLTDPDGNMAFFWHFILTFSEARNAGVGFLDSLKMAWHAMAW
                     DFRGTQSTTDEYMVNSHAMIRGSITNEAGKIVREQQTIEEAAQGTEKFIKEKVKEGTI
                     RSIGEAAHAAVDKATESHKEQVWTGELTWEHIKKDTFPSKEAIKEARRNIQEVLQEAF
                     KINPDLKSIK"
     gene            3935..4135
                     /locus_tag="PLV81_13850"
     CDS             3935..4135
                     /locus_tag="PLV81_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11221.1"
                     /translation="MKVDVTKDELYSMIKEAVREVISEKQLHIILHSLPYISDDEMRQ
                     IYEQYGSPEQYSDVAFSEKIDI"
     gene            4137..4400
                     /locus_tag="PLV81_13855"
     CDS             4137..4400
                     /locus_tag="PLV81_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOM11222.1"
                     /translation="MDNILFKPCRKIFKKHPTYSSHIKETITLLAKKLSGEITSLDVC
                     KMKGVWEGHLRIRKGDIRIILSVDEHDKIIHVKVIDFRGDVYK"
     gene            complement(5408..6052)
                     /locus_tag="PLV81_13860"
     CDS             complement(5408..6052)
                     /locus_tag="PLV81_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11223.1"
                     /translation="MVTIFKRGIAVIVIAGVIGAWGCKPKEPKGEKNAVPPEITQFVD
                     ALFQAIVNEDIEYIEKVLYDGEIEYDYNEVLGDYIYKPKSDIIKEIRNKGKTYYELFD
                     TERLIEYIAKYRCSDGKGNPCTVENIKLSAPYIQSVKEKILSRKNIRVEKIEVIDKEK
                     NIIRCHLIDDYEIEMKKKSPLSEMIFLKKVDIIKNEHGIYLYSLFFDVGPIVED"
     gene            complement(6064..>8213)
                     /locus_tag="PLV81_13865"
     CDS             complement(6064..>8213)
                     /locus_tag="PLV81_13865"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOM11224.1"
                     /translation="KWINYVYNYKQVKMIDNPDSEDVYYQYGTAGDGHNGAGRVVEEK
                     TGILSDSYWYGALGQVIKKERSIDGKTYRIQWQWDNFGRVRSINYSNGFSVYYAYDAG
                     GQVGGVVGYCGSLRTDYVKAIHYDEYGSRTKVEYGNGVESTYRYDERMHRLVQLQTKQ
                     GDSTLQNIAYSYDRVGNILTRTENGIQMSDRTPKTITHRYSYDSLYRLVEAEGTLQEN
                     GNVVNNYRNAFTYSEIGNILSKLQIVKAQGNEDPTLTYTYTYTYAGNKPHAVTGINDN
                     LTYRYDANGNMTAVYDTAKNYNRILYWDDDNRLTKTVDTQAGVSTVTQYAYDAKGMRI
                     IKDGPYGKSIYVYTGYVLSQEYVESNHIFVGNTRVASIVKHKDETKPATYYYATDHLG
                     SSSVLTTSTGSYHERIEYLPYGETWFEDAVNSNAYSTPYKFTGKELDKETGLYYFGAR
                     YYDARISMWVSTDKYLEQYLPLKGNDNITKLPGNGGVYTSINMDVYNYGNENPLRYID
                     PDGNSALDKILDYIRNIFTTSTVTQQQEDDALHGRGNYQSPLRGFDPEKGNGVTSDFG
                     MRTRPGTKRTEPHVGVDIAASEGTPIYAAHSGIITYNTKIITDNNGNIIGGFGNYAVI
                     RGAHGIETVYGHMTDESMALLPNGTQVQQGQLIGYVGKTGFATGPHLHFEVRHLYNGH
                     NPFPAIPSYPVNPQMNQYIYYNPRRFLEYMMNR"
BASE COUNT         1968 a         1985 c         1396 g         2864 t
ORIGIN      
        1 aaactccacc tcttcctcgt tttccattta aatattcttc aattattgga tccgtgctta
       61 tccacctgct cagccgtgca tcgtagtaac gggctccaaa gtagtatggg cctgtttctg
      121 cgacatactc cttaccttta tttcagccat ttacacaaat ttctctacaa gatcatgatt
      181 ttggtcaaat atccgatagc aaatcttacc acaactcctc tgctggtatc tcataatgaa
      241 atcttttcca aggaggacaa ctaatgtttt caatcacttt gaatctttga tattctggat
      301 aatcaggcaa tgtgccatat ttataccatt taagtccttt ctctgtgata ttcacgtctt
      361 taatatcagt ccatttggtt ggagcgattt gtacccctgc cttgattaca tcactaatct
      421 tctcatttct atttaatcca tcgccatacg aaatacctct cccaccatac catttaatat
      481 ttatttcacc acatgccata ttcatatttc cagtattaaa acacaaacgg cctattatgc
      541 aaaatgagtt atatgttgct tttcccatcc catgctttgc tgtcttgatt aaaaaatctc
      601 ctgtaccatc tccttggtaa taatattcat aaattgcata tctcttatct tttttatggt
      661 gttggccagt ttccaaaaac ctgactgcgc atagtttgtt atccttcttt actaaagcat
      721 aatagccaaa aggcatttca atttctgtat caaatatttt tattataggc ttctcattta
      781 atggtggttt ttgtatattc atttgatcac catacagtaa aactgataag aatagaagat
      841 gaagaataat tgacagagtt aatatcgatt tttccatctt gtattaaccc tactttatag
      901 atttaagatc aggattaatt ttaaaggctt cttgcaatac ttcctgtata ttccggcgcg
      961 cttcttttat tgcttctttg cttgggaatg tatccttttt tatatgttcc cacgttaact
     1021 ctcccgtcca cacttgctcc ttatggcttt ctgtagcttt atcaaccgct gcatgagctg
     1081 cttctcctat acttctaatt gtcccttcct tcactttttc tttaatgaat ttctcagtgc
     1141 cttgtgccgc ttcttctatt gtctgttgct ctcttactat tttaccagct tcatttgtta
     1201 tacttcctct aatcattgca tgagaattca ccatgtattc atctgttgtg ctctgtgtgc
     1261 ctctaaagtc ccacgccata gcatgccatg ccatttttag gctatctaaa aatcctactc
     1321 cggcatttcg cgcttctgaa aacgtcagta taaaatgcca gaaaaaagcc atgttgccgt
     1381 caggatcagt taattgaacc ggattattcc ccgcataatg atatacatcc atatttattg
     1441 cattaaacac ccctcccata ccggcaagct ttcggctgcc atcctgctgt aaataccagt
     1501 agaaatcatg ctcggtatca taattattta gcttggggaa gtatttgcct tcctgcagcg
     1561 ccgggtcggt gcttatccac ctgcttatcc gtgcatcata gtacctggct ccaaagtaat
     1621 acagtcctgt ttctttatcc aattccttgc cggtgaattt atatggtgtg gtgtagccgt
     1681 tgctgtttac agtatcctct acccacactt caccatacgg cagatactct atccgctcgt
     1741 ggtatgcacc aatattattt gtgagcaccg acgatgagcc aaggtgatcc gtggcgtagt
     1801 aatatgtggc tgcttttgtt tcatccctgt gtttcaccac gcttgccact ctggtattgc
     1861 ctacaaatat atgattgctt tcaacatact cctgtgacag cacatatcct gtatcaacat
     1921 aaattgattt cccatacggg ccatccttca ctatacgcat gccctttgca tcgtaggcat
     1981 actgtgttgc cgtacttacc cctgcctgtg tgtccaccgt ctttgttaac cggttgtcgt
     2041 catcccagta taatattctg ttataattct ttgctgtgtc atatactgct gtcatgttgc
     2101 cgtttgcatc atagcggtat gtcaggttat cattgattcc tgtcaccgca tgcggcttgt
     2161 tccctgcgta ggtgtatgtg taggtgtagg tgagtgtggg atcttcgttt ccctgtgctt
     2221 tcacgatttg tagcttactc aatatgttgc caatctcgct gtaggtaaat gcattgcgat
     2281 agttgttaac aacattgcca ttttcctgca gtgtcccttc ggcttccacc agccggtaca
     2341 aactatcata gctataccgg tgtgtgattg tctttggggt tctatcgctc atctgtatgc
     2401 cgttttctgt ccgtgtcaat atatttccta ccctgtcata actgtaggct atgttttgta
     2461 aggtactatc gccctgcttt gtttggagct gcacaagcct gtgcatacga tcgtcatact
     2521 catacgtgct taccactcca tttccatact ctacccttgt tctacttcca tattcatcat
     2581 actgtatgtc ctttacataa tctgtccgta ttgcgcccaa ataccccact actcctttca
     2641 cctgaccgcc ggcatcatac gcataataca cactaaaatt gttgctatag atgatgctgc
     2701 gaactctacc aaagttgtcc catttccact ggatgcgata tgtcttccca tctatgcttc
     2761 gctcttttgt tatgacctca cctaacgccc cgtaccagta actatcgaca agcttccctg
     2821 tcttttcctg taccacgcgg cctgctccgt ttttaccatc accaggtcta ccatattcat
     2881 aataaacatc ttccgaatct ggattgtcaa tcatttttac ctgcttgtag ttgtacacgt
     2941 aatttatcca tttgttttgc ttgcgcaggt ttgccgtttg cttgcgtatc aggtttcctg
     3001 cgtcatcgta ctggtaaatt gttttgccgg tgtcagggtt gtcaatgctt gtgcgcctgc
     3061 cccaggtgtc atacgtaatg ctggttacat tccctgccgc atccaccacc gtgaccagct
     3121 gattgaccac atcataccta taggtagtgg taatgccgcc ttcctccgta acagcaacga
     3181 tgtttccccg cacgtcgcgg gttatgagct tgctattgcc attctgatcc gtgatgcact
     3241 caattaattt cccatctcgc gtgccgtacg ctgctactac aataccacca tcgggatagg
     3301 tggcttctat ctttcgtccc atgcagtcat actgataggt ggtgggatac ttcagtgcca
     3361 ccggactata ctcaaaacta ttcccttcct caaacgccgg tttgccctct gctaccactc
     3421 tcccctgtgc atcgtacact cgtgccccac ttaccgtaaa gccatacttc ccttctacct
     3481 gtgcctgcgt ctttacctgt agctctcgcc ctaacccgtc tgccatccgc actatggcta
     3541 ttgtctcgtt gtagcggcca ccactgcggt tatacgttac tgctcttgct ggagcggtta
     3601 tcccttctgc agttactatc gccccagcat actgtacctc tatggctgct ctgcctgttt
     3661 ccatgtcata cggcccccat acgtgcgtta gccgcccaaa ctggtcatga tgatactgga
     3721 aactgtagcc attgatgtca cgcgtccagt acggcgtgcc aaaggccgga ttgtactgtg
     3781 cggctgcggt gtagccgtag gcgtcagtgg tcttttctac gtagatgccc gtggcgtcgt
     3841 aggtatttat ttttttcttg atttttccgt tatttatcca tattttagtt aaaaatattt
     3901 atttttaata ataacgatac agcaggttat gtttatgaaa gttgatgtaa caaaagatga
     3961 actttattca atgattaaag aagcagtacg agaagtaatt tccgaaaaac aattacatat
     4021 aattctacat tctctcccgt acatctctga tgatgaaatg cgccaaattt atgaacaata
     4081 tggctcacct gaacagtata gtgatgtggc attctctgaa aagatcgata tatgaaatgg
     4141 acaatatact attcaagcca tgccgaaaaa tttttaagaa acaccctaca tattcatccc
     4201 atataaaaga aacaattaca ttgctagcaa aaaagctaag cggtgaaata acatcgttag
     4261 atgtttgtaa aatgaaaggt gtatgggaag gtcatcttcg tattcgtaaa ggtgatattc
     4321 gcattattct atcagttgat gaacacgata aaattattca tgtaaaagta atagacttta
     4381 gaggtgatgt gtataagtaa gaaaatttag tttgaaaaat aataaatata aattatgaaa
     4441 atttttaaat taaaatttgt ttaactatcc gtatttatat gagtatattt gttttttatt
     4501 cctagttata tgtatcattt attttatgtt atttattacg taattctttt acatgcaaat
     4561 tagtgatttt acccaccttt ttcgcacttt attttgtcca gacggcaaca atcccataat
     4621 attcttttca caaggggaaa aagggcttca tctcgacagg ctcgatgacc ggccagcccc
     4681 ttttcccctt gtcatcccct ttaccccggc ttggcgccta tcgttttttg tccctcattt
     4741 catgagggat tccctgcgct cctcgtccac agcgggggct gcggaacttg caccgattgc
     4801 atcggtgctt cgaacagtcc tcgccctttt cccgccgtgt cctgcggtgc tcggcaaaaa
     4861 tacggcgcct gtactgatcc tatctaatga aggatggtga gtacttcaac aagctcagtg
     4921 accccctgtc gaagcacggt tggtgagcac ttcggcaggc tcagtgaccg cctgtcgaac
     4981 catggttgct gagtttatcg aagcacggtt ggtgagcttg tcgaaccacg cacgcaaccc
     5041 tatgacgccg ttcatcctgt acggctggca ttgctcaact gatgctttag cattatctat
     5101 gaaataacat ccttaatgat gtaactacag ttatatccgc ttcatttttt tgctttcaat
     5161 aattttaata acagttattc aatgttatcc attaccacga tatactacat attatcgtaa
     5221 cacactcatt taaaaggtac tttacaaaaa tgcatcataa tactttttgt taaattttga
     5281 atgaactatc ttcattattt aaacttctat tttatgcata tactgttgat taataacatt
     5341 tcaaataatc aatctttttt atcgttttag atgttgatta caaaaaaatt actttttttt
     5401 aaaatcttca atcttcaaca atgggaccaa catcaaaaaa taaactatat aaatatattc
     5461 catgttcatt tttaattata tcaacctttt ttaaaaatat catttcactc aatggacttt
     5521 tcttcttcat ctcaatttca taatcatcta ttaaatgaca ccgtattata ttcttctcct
     5581 tgtctattac ctcaatcttc tctacccgta tattcttcct gctcagtatc ttctccttca
     5641 ctgattgtat ataaggagct gacaatttta tattttcaac agtacaaggg ttcccttttc
     5701 catcgctaca tctatatttt gctatatatt caattaatct ctctgtatca aataattcat
     5761 aatatgtttt tcctttattc cttatttcct taattatatc actctttggt ttatatatat
     5821 agtcaccaag aacttcatta taatcatatt ctatctcccc gtcatataat accttctcta
     5881 tatactctat atcttcattg actatcgctt gaaataatgc atctacaaac tgcgttatct
     5941 ctggcggaac tgcattcttt tccccctttg gttccttagg cttacatccc catgctccta
     6001 ttactcctgc tattactata acagctatcc ctcgtttgaa tattgtaacc acaccatacc
     6061 tccttaccta ttcatcatgt attctaaaaa tcgtcttgga ttgtaatata tatactgatt
     6121 catttgcggg tttactggat aacttggaat agcagggaat ggattatgcc cattatacag
     6181 gtgccgtact tcaaagtgca aatgtggtcc tgttgcaaac cctgtcttgc caacataccc
     6241 tatcaattgc ccttgctgta cctgggtgcc atttggcagt aatgccatac tttcatctgt
     6301 catatgtcca tacactgttt ctatcccatg tgctccccgt atcaccgcat aattaccaaa
     6361 gcccccaata atattcccat tattatcagt tattattttt gtattatatg tgataatacc
     6421 agaatgcgcg gcatagattg gcgttccttc tgatgctgct atatccactc ctacatgcgg
     6481 ttctgtcctt tttgttccag gtcttgttcg cataccaaag tctgatgtta ctccatttcc
     6541 tttttcaggg tcaaacccac gtagtggtga ctgataattt ccccgtccat gtagtgcatc
     6601 atcttcctgt tgttgtgtta cagtgcttgt tgtgaatata tttcgtatat agtccaatat
     6661 cttatctaat gctgaattcc cgtctggatc tatataccgc agcggattct catttccata
     6721 attgtataca tccatattta ttgaagtata tactccccca ttaccaggta gttttgttat
     6781 attatcatta cctttcaatg gcaggtattg ttctaaatat ttatccgtac tcacccacat
     6841 gcttatccgt gcatcgtagt accgggcgcc aaagtaatac aggcctgttt ctttatccaa
     6901 ttccttaccg gtgaatttat atggtgtgct gtaggcgttg ctgtttacag catcctcaaa
     6961 ccaggtttcc ccatacggca aatactctat tcgctcgtgg tagcttcccg tgctggtggt
     7021 tagcaccgag cttgagccaa ggtgatccgt tgcgtagtaa tatgttgctg gtttggtttc
     7081 gtctttgtgt tttactatac ttgctaccct ggtgttgcct acaaatatat gattgctttc
     7141 aacatactcc tgtgatagca catagcccgt gtaaacataa attgattttc catacgggcc
     7201 atcttttatg atgcgcatgc cctttgcatc gtacgcatac tgtgttactg tacttacccc
     7261 cgcctgtgtg tccaccgtct ttgttaaccg gttgtcgtca tcccagtata atattctgtt
     7321 ataattcttt gctgtgtcat atactgctgt catgttgccg tttgcatcat agcggtatgt
     7381 caggttatca ttgattcctg tcaccgcatg cggcttgttc cctgcgtagg tgtatgtgta
     7441 ggtgtaggtg agtgtgggat cttcgtttcc ctgtgctttc acgatttgta gcttactcaa
     7501 tatgttgcca atctcgctgt aggtaaatgc attgcgatag ttgttaacaa cattgccatt
     7561 ttcctgcagt gtcccttcgg cttccaccag ccggtacaaa ctatcatagc tataccggtg
     7621 tgtgattgtc tttggggttc tatcgctcat ctgtatgccg ttttctgtcc gtgtcaatat
     7681 atttcctacc ctgtcataac tgtaggctat gttttgtaag gtactatcgc cctgctttgt
     7741 ttggagctgc acaagccggt gcatacgctc gtcataccga tacgtgcttt ccacgccgtt
     7801 gccgtactca accttggtgc ggctgccgta ttcgtcgtag tgaatagctt tcacataatc
     7861 ggtgcgtaaa ctcccgcaat agcctaccac gccacccacc tgaccgcctg cgtcatacgc
     7921 atagtataca ctaaagccat tgctgtaatt gatgcttcgc actctgccaa aattgtccca
     7981 ttgccactgg atgcgatatg tcttcccatc tatgcttcgc tctttcttta ttacctggcc
     8041 taacgccccg taccagtaac tatcgctcaa aatccctgtc ttttcctcta ccacccgacc
     8101 ggcaccattg tgcccatcgc ccgcggtccc gtactgataa tatacatctt cagaatccgg
     8161 attgtcaatc atttttacct gcttgtagtt gtacacgtaa tttatccatt tgt
//
LOCUS       DAORYL010000107         7844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_6629_length_7844_cov_6.524586, whole genome shotgun sequence.
ACCESSION   DAORYL010000107 DAORYL010000000
VERSION     DAORYL010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7844
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6629_length_7844_cov_6.524586"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PLV81_13870"
     CDS             complement(<1..400)
                     /locus_tag="PLV81_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3644 domain-containing protein"
                     /protein_id="HOM11225.1"
                     /translation="MRGQPVIVKQLLQKAKDSALLAVEFYNKPAVSFKSEGYITMMCI
                     AWTSLLHAYFLKNKIKPYYRKKSSGSKRPRFEQIIETLPGGQKVKENRWWELQECVKQ
                     YFKDDNNSPIRKNLEFFIPLRNMIVHRNLPE"
     gene            complement(420..4028)
                     /locus_tag="PLV81_13875"
     CDS             complement(420..4028)
                     /locus_tag="PLV81_13875"
                     /inference="COORDINATES: protein motif:HMM:NF019291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HOM11226.1"
                     /translation="MQCGNIDVAVFSCRVEKNLTERSSKKAQYVLGKKILRQLSRYAG
                     GFFIFYDANCNFRFSFIYSLPRADGRQDWSNFRRYTYYVSKDQTNKTFKQQMVAGDFN
                     SIDSIIDAFSVEKVTREFYQEIANWYFWALKNVKFPQDVENEPNGKEMAVIRLITRLI
                     FIWFMKIEGLIPAQLFDEIFIKDILKNYKPNNDDYYKAILQNLFFATLNTKQENRRFR
                     SNIRGHKGYNPDFGNNYVYRYEDLFKESEKIIKEYFLQIPFLNGGLFECLDHKSKDEL
                     RYIDGFTDVVKHQPKIPNYLFFSNEEIVDLNSDYGTKNKKYKVRGLINIFRSYNFTIN
                     ENTYDDAEVALDPELLGKIFENLLASYNPETAKTARKETGSYYTPREIVDYMVEQSLR
                     EYFKTNMYSINDKELDELFSPINEGKSINLDEYTKKSLIELIYNLRVLDPAVGSGAFP
                     MGMLHKLVLLLQKLDPDNEQWKTMQIEDVKRNIKDPTLVSKFIKQIEENFAEKNDDYG
                     RKLYLIEKCLYGVDIQPIAIEIARLRFFISLLVDEKINKNKENYGIEPLPNLDFKLMQ
                     GNSLITSFYGIDLRYNESIKTLREKLNPKITNLEILKHQYQYACDVKDKKDLMESIEN
                     SILDIFKTYIEIYFQHHLSYIKNSFIFENKELMEGELKNYYKQEGFDIEKAIDELKDY
                     IELKKPKNFFLWNIFFAEVFNEKGGFDIVIGNPPYVSTKGVMEKEKPILGKIYGFADD
                     LYSHFYFKGIELLKGNGILTYISSKTFWTIQTKKNLREFLLKNKLIQIIDTANPFESA
                     MVDTCITIVQKEANDKNIIRFIDVKNGWENIKEYQVEPQLYKDMVNNVFFIPTKFNLK
                     VYERLGKIVKQLMDKWWDKIDTSKNIEKNKRELEQYRNSLKPGDITLLGLITEGGQGL
                     ATANNGKYVGVLEGTKWADNVRKQRPEKLLLATNFCKKEGIRTKADAQSLLNKLNENE
                     IRKLFDDLKEKYGRDIFGQGWLYRIVSPDEIANVDTLTDDEKLNGIKGEKTFVPYDKG
                     DKDGNRWYAPTPYYIDWSRENVKILQTDPKARWQGYQFYFREGFCWTDVNSTYLKSRL
                     KENGVYDVLTMSLFTSTAYPDWYFVCMINSKFISEYVDDFVNSTSHFQINDARQLPII
                     IPTTEQLMVFEDIFNSAVSVQKEKFAGKLSEQNAEAKLADIQRELDERVLEMYGVR"
     gene            complement(4188..7586)
                     /locus_tag="PLV81_13880"
     CDS             complement(4188..7586)
                     /locus_tag="PLV81_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOM11227.1"
                     /translation="MSNFITNDQPKNLLKRLQQLIAVSEELKFLVGFFYFSGIKELYE
                     SLLQNSQLKLNILVGLNVDIVNYQLVECADASDKNLSNAGIAGRFFQSIKNSINSDEF
                     DTEEFYKQITFFKQLIKNGNCIIRKTIKPNHAKLYIFKLKQGQVKRSLFITGSSNLTR
                     AGLVDQSEFNVEISDYGIEEAEKYFDDLWNKAVKITEDEVRKQQLISILENDTFIKEI
                     TPFEAYCLVIKTFLDSYEAVTIGDYLCKILEKNGYTPYRYQLDAVSQALSIIQKHNGV
                     ILADVVGLGKTIIACAIAKQLKKRGIVICPPGLVGDTNKTAGWSKYLEDFQLFDWEAR
                     SLGDLENVLEFVNGHDDIEVIIIDEAHRFRNQDTRAYELLKNICRNKIVIALTATPFN
                     NKPGDIFSLIKLFVTPKKSTVTLDNNIEYLFSEYRRYFDRLSYIKRYYNSSDKQKRER
                     AKVQYKILFSDTSIDLNKVNRATHRLAKEIREVIEPITIRRNRIDLQKNPYYKDEVKQ
                     LSKVCDPIEWFYELSPQQLQFYDTVIQKYFGDTDDGGLFKGAIYRPFVYEEGELQNDL
                     EEATNENQMEKNREYFQQMNLFHFMRRLLVKRFESSFGAFKQSIENFKKIHQDVLQFI
                     TKTGNGNPINGEFILDRALLEKIYDLDLDEIEGYLIQYEEQINKGVLPKKHKRYKIKD
                     FKYADEFINDIKNDINLFDKILEELNTLDLVNNDPKLECLIQNIKNEFSKKPKNNEPK
                     RKIVIFSEFKDTVKYVEDTLNQAFDERVLVVAGDLPPSKIQSIYKNFDASFKNQEDDY
                     DILLCTDKISEGFNLNRAGMIINYDIPWNPVRVIQRLGRINRISRKVFNELYIVNFFP
                     TEKGAEIVKSREIAQNKMFMIHNTLGEDAKIFDSDEEPTPAELYNRLQQNPETREAES
                     FYTKVLNIYLQLKEKYPDIIKNLHNLPRRIKVSRRSNVDELLVFFKKNRLYVMQYDYY
                     EKEIRQTSLEEVLERMQCSIHETSLPIDERFWSAYEEIKNAKEKKGGPSSELSLYRRA
                     LGKLNFLISNNDVEEINQYKEFLMLLREDILDYGTLSEYTLRRIANFKTDFKNENEKK
                     QLLGDIDLLRCELGDSYLVKEKYKHNGLQKEIIIAIENKILKEE"
BASE COUNT         2383 a         1421 c         1004 g         3036 t
ORIGIN      
        1 gttctggtaa attacgatgt actatcatat ttcttaatgg aataaagaat tctaaatttt
       61 ttctaatagg tgagttattg tcatccttaa aatattgttt tacgcattcc tgcaattccc
      121 accatctatt ttcttttact ttttgaccgc ctggcaatgt ttctattatt tgctcaaatc
      181 tgggtctttt tgaaccgctg cttttctttc tgtaataagg tttaatttta tttttcaaaa
      241 agtatgcatg caataatgat gtccatgcaa tacacatcat tgtaatgtaa ccctctgact
      301 taaacgaaac agcaggcttg ttgtaaaatt ctactgccag taatgcagaa tcctttgctt
      361 tttgaagaag ttgttttaca attacgggtt gacctctcat ataatatttt ttattttttt
      421 catctcaccc catacatctc caacacccgc tcatccaact ccctctgtat atccgcaagt
      481 tttgcttctg cattttgttc gcttagctta ccagcaaatt tttccttttg caccgaaacc
      541 gcactgttaa atatatcctc aaataccatt aattgctctg tagtgggaat aattatcggc
      601 aattgacggg catcgttaat ttgaaaatga gaagttgaat ttacaaaatc gtcaacatat
      661 tcactaataa attttgaatt aatcatacac acaaaatacc agtcaggata tgctgtgctt
      721 gtaaatagtg acattgttag aacatcatat acaccatttt cttttaatct tgattttaaa
      781 taagttgagt taacatctgt ccagcaaaaa ccttctctga aatagaattg ataaccttgc
      841 cagcgggctt ttgggtcagt ttgcaatatt tttacattct ctctgctcca atcaatataa
      901 taaggcgttg gggcatacca gcggttgcca tctttatcgc ctttgtcgta tggtacaaag
      961 gttttttcgc ctttaattcc gttaagtttt tcgtcgtcgg taagtgtatc cacgttggct
     1021 atttcatcag gacttacaat acgatagagc caaccctgcc caaatatatc tctgccgtat
     1081 ttttctttca aatcatcaaa aagcttgcga atttcatttt cattcaattt attcaacaat
     1141 gactgcgcat cagctttagt tcgtatacct tcttttttac aaaaattagt tgccaataaa
     1201 agtttttcag gtcgttgctt acgcacattg tctgcccatt tggtgccttc caatacgcct
     1261 acatatttac cattgttagc agtagcaagt ccctgtccac cttctgtaat cagtccgagc
     1321 agggtaatat cgcctggttt tagtgagtta cgatattgtt caagttctct tttattcttc
     1381 tcaatgtttt tgctggtgtc aattttatcc caccatttat ccatcaattg tttaacaatt
     1441 ttacccagtc tttcataaac cttcaaatta aatttagtag gaataaaaaa tacattatta
     1501 accatatctt tatataactg tggttcaacc tgatattctt ttatgttttc ccatccgttt
     1561 ttaacatcta taaaacgaat tatattttta tcgtttgctt ctttttgaac aatagtaatg
     1621 caggtatcaa ccattgcact ttcaaatgga ttagcagtat caataatttg tattaattta
     1681 ttcttaagca gaaattctcg taaattcttt ttagtttgta ttgtccagaa tgtttttgaa
     1741 gatatatatg taagaatgcc atttcctttt aataattcaa tacctttaaa ataaaaatga
     1801 gaatataaat catcagcaaa accataaatt tttcccaaaa ttggtttttc tttttccatt
     1861 acacctttag tactcacata aggcggatta cctattacaa tatcaaagcc acctttttca
     1921 ttaaatactt cagcaaaaaa tatattccat aaaaagaaat ttttaggttt ttttagctca
     1981 atataatctt ttaattcatc tattgccttt tcaatatcaa atccttcttg tttataataa
     2041 ttttttaatt ctccttccat tagctcttta ttttcaaaaa taaaagagtt tttaatgtat
     2101 gataaatgat gttgaaaata tatttcaata tatgttttaa atatatcaag tattgaattt
     2161 tctatggatt ccattaaatc ctttttatct ttaacatcgc atgcatattg gtattggtgt
     2221 tttaatattt caagattagt aattttagga tttaattttt cccttaatgt ttttatactt
     2281 tcattatatc gtaaatctat accatagaaa ctggttatca aactattacc ctgcattaat
     2341 ttaaagtcca ggttcggcaa gggttctatt ccataatttt ctttattttt atttattttt
     2401 tcatcaacta acaatgaaat aaaaaatcgt aaccgcgcta tttctattgc aattggctga
     2461 atatctactc catataaaca tttttcaatt aaatataatt ttcttccata atcatcgttt
     2521 ttttcagcaa aattttcttc tatttgtttt atgaacttac ttaccagcgt aggatcctta
     2581 atgtttcttt ttacatcttc aatctgcatg gttttccatt gttcattgtc aggatctaat
     2641 ttttgaagta ataataccaa cttatgcaac attcccattg ggaaagcacc tgaaccaaca
     2701 gcaggatcta gtactcttaa attgtatatt aattcaatga gtgatttttt tgtatattca
     2761 tctaaattta ttgatttacc ttcattaata gggctaaaca attcatctaa ttctttgtca
     2821 ttaattgaat acatattagt cttaaaatat tcacgcaatg attgttctac catataatca
     2881 acaatttcac gtggtgtata ataacttcca gtttctttgc gtgctgtttt agcagtttca
     2941 ggattatagc ttgctaaaag gttttcaaat attttaccca ataattctgg atcaagtgca
     3001 acttcagcat catcataagt attttcattt atagtaaaat tatatgatct aaaaatattt
     3061 attaaacctc taactttata tttcttattt tttgttccat aatcgctgtt aagatctacg
     3121 atttcttcat ttgaaaaaaa taaataattt ggaatttttg gctggtgttt taccacgtca
     3181 gtaaatccat caatatatct caattcatct tttgatttat gatccagaca ctcaaataat
     3241 ccaccattta aaaatggtat ttgtaaaaag tattctttaa ttattttttc actctcttta
     3301 aaaagatcct catatctata aacataattg ttaccaaaat ctggattgta ccccttatga
     3361 ccccttatat tacttctaaa acgcctattt tcttgttttg tatttaatgt ggcaaaaaat
     3421 aaattctgta aaattgcttt ataataatca tcattatttg gtttataatt ttttaaaata
     3481 tctttaataa atatttcatc aaatagttga gcaggtatta aaccttcgat tttcataaac
     3541 caaataaata ttaatcttgt tattaacctt attactgcca tttctttacc attaggttca
     3601 ttttcaacat cttgcgggaa tttaacattt tttagtgccc agaaatacca gttggctatt
     3661 tcctgataaa actccctggt aaccttttca acactgaatg catcaattat tgaatcaata
     3721 ctattgaaat cacctgcaac catctgctgc ttaaaggttt tgtttgtctg gtccttgctt
     3781 acatagtacg tgtaacgtct gaaattgctc caatcctgcc tgccgtctgc tcgtggtaaa
     3841 ctgtaaatga atgaaaatct gaaattgcaa ttggcatcat aaaaaataaa aaatccacct
     3901 gcataccgtg aaagctgacg caatatcttt ttccccagta catactgggc tttcttgctg
     3961 ctacgctcag ttaaattttt ttcaacccta cacgaaaaaa ctgcaacgtc aatattacca
     4021 cattgcatct gccccaattg ttttacatta caaaatagtt cattatcaaa ttgtgacagt
     4081 tcatgttcaa caggttcata gttatcgttt ttttgcctga aaaattcttc aaataaattc
     4141 aggttaaaat cattaataat attttcaagt attgtagtag tcatatacta ttcctctttt
     4201 aatattttgt tttcaatagc aataatgatt tctttttgca aaccgttatg tttatatttt
     4261 tcttttacaa ggtaactatc gccaagctca caacgcaata aatcaatgtc ccccagcaat
     4321 tgcttctttt cattttcatt tttaaaatct gttttaaagt tagctatgcg gcgtaatgta
     4381 tactcagaaa gtgttccata atcaagtatg tcttctcgta atagcatcaa gaattctttg
     4441 tattgattta tctcttcaac gtcattatta cttataagaa agtttagttt acccaatgcc
     4501 cgcctgtaca ggctaagttc acttgagggt ccaccttttt tttcttttgc atttttaatt
     4561 tcctcatacg cgctccagaa tcgttcatca atgggtaacg atgtttcatg tatactgcat
     4621 tgcattcgct ccagtacttc ttcaagcgac gtttgacgta tttctttttc ataataatca
     4681 tactgcatca catataatct atttttctta aaaaatacca gcaattcatc aacattgctc
     4741 ctgcgagaaa cttttatacg tcgcggcagg ttgtgtagat ttttaattat gtcaggatat
     4801 ttttctttaa gctgcaggta aatatttagc actttggtat aaaagctttc ggcttcacgc
     4861 gtttcagggt tttgctgtaa tctgttgtaa agctctgcag gtgttggctc ttcatcacta
     4921 tcaaatatct tggcatcttc acccagggta ttatgaatca taaacatttt gttttgcgca
     4981 atttcacgcg atttaactat ctcagcaccc ttttcagtag gaaaaaagtt tactatatac
     5041 agctcattaa aaacctttct gctaatacgg ttaattcgtc caagcctttg aattaccctc
     5101 acaggattcc agggtatatc ataatttata atcatacctg cacggtttaa gttaaaacct
     5161 tctgaaattt tatcagtgca caacaatata tcgtaatcat cctcctgatt tttaaaactg
     5221 gcatcaaagt ttttataaat ggattgaatc tttgatggtg gaaggtcgcc agcaacaacc
     5281 agcactctct cgtcaaaagc ctgattcaat gtgtcttcaa catatttaac ggtatcttta
     5341 aattcagaaa atataacaat tttgcgcttt ggttcgttat tttttggctt ttttgaaaat
     5401 tcatttttta tattttgtat taagcattct aattttggat cattattgac aaggtctaat
     5461 gtattaagct cctcaagtat tttatcaaat aaattgatat catttttaat gtcattaata
     5521 aattcatctg catatttaaa atctttaatt ttatatcgtt tatgtttttt aggtaatact
     5581 cctttattaa tttgctcttc atactgaata agataaccct caatttcatc aagatccagg
     5641 tcataaattt tttcaagtaa tgccctatct aaaataaatt caccattgat gggatttcca
     5701 ttacccgttt tagtaataaa ctgtaacaca tcctgatgta tctttttaaa attttcaata
     5761 ctttgcttaa atgccccaaa cgaactttca aaacgtttaa ccagcaatcg cctcatgaag
     5821 tggaataagt tcatctgttg aaaatactca cgattctttt ccatctggtt ttcatttgta
     5881 gcttcttcca ggtcattttg caattcacct tcttcatata caaatgggcg gtagatagct
     5941 cctttaaata atcccccatc gtctgtatcg ccaaaatact tttgaataac cgtatcgtaa
     6001 aattgtaatt gttgtggcga tagttcatag aaccattcaa ttggatcgca tacttttgat
     6061 aattgcttaa cttcatcttt ataatacgga tttttttgta aatcgatgcg attacggcgt
     6121 attgttattg gttcaataac ttcacgtatt tcctttgcta aacgatgtgt tgcacgatta
     6181 actttgttta aatcaatgga agtatcgcta aatagaattt tatattgtac ttttgctctt
     6241 tcacgctttt gtttatcaga actgttataa taccgcttta tgtagcttaa cctgtcaaaa
     6301 tatcttctgt actctgaaaa caaatattca atgttattat ccagtgttac cgttgatttt
     6361 tttggggtaa caaataattt tatcaaagaa aaaatatcac ctggtttatt gttaaatggt
     6421 gttgcggtaa gtgctattac aattttattg cgacatatat tttttaatag ctcatacgca
     6481 cgggtatcct gatttctgaa acggtgggct tcatcaataa ttatcacttc aatatcatcg
     6541 tgtccattaa caaattcaag aacattttct agatcaccta acgaacgggc ttcccagtca
     6601 aaaagctgga aatcctctaa atacttactc caaccagcag ttttattggt gtcaccaact
     6661 aggccaggtg ggcaaatgac aataccacgt ttttttaatt gctttgctat ggcacaggca
     6721 atgatggttt tacctaaacc aacaacatca gcaagtataa caccattatg tttctggatt
     6781 atagacagcg cctgacttac cgcatctaat tgatacctgt aaggtgtata gccatttttt
     6841 tctaatattt tacataaata atcaccaata gtgactgcct cgtatgaatc aaggaatgtt
     6901 tttataacaa ggcaatatgc ttcaaaaggg gttatttctt taataaaagt gtcattttcc
     6961 agtattgaaa ttaattgttg ttttcttact tcatcttcag ttatttttac tgccttattc
     7021 cataaatcat caaaatattt ttcagcttct tcaattccat aatcagatat ctcaacatta
     7081 aattcactct ggtcaaccaa ccctgctcgt gttaaattac tgcttccagt aataaataaa
     7141 gaacgcttaa cctggccctg ctttaattta aaaatatata atttagcatg gttgggtttt
     7201 attgttttgc gtataataca attgccattt tttattaatt gtttaaaaaa tgttatctgc
     7261 ttgtaaaatt cctcagtatc aaactcatca ctatttattg aatttttaat tgattggaaa
     7321 aacctgccag caattcctgc atttgataga tttttatcac tggcatctgc acattcaaca
     7381 agctggtaat taacaatatc aacattcaat ccaaccagta tattcagttt taactgtgag
     7441 ttttgtaata acgattcata taattcttta attcctgaaa aataaaaaaa acctacaaga
     7501 aattttaatt cttcactgac agcaattaat tgctgcaacc tttttaataa atttttaggc
     7561 tgatcattgg taataaaatt tgacattatt attccctttt atttcagata ttcaaaattt
     7621 ttaattcctt aatatttatc atttattatt tattatttgc ataaccattt gccattaaac
     7681 aaaattttca ttatccttcc attttaacgg attgtttatt atatatgaac gtatattgta
     7741 taattccata tcattgcgta taatatgttc ataataattg cgttgccaca atttgccatc
     7801 aaatcgaggc catttatttt gtttaacacc acgtatatat tcat
//
LOCUS       DAORYL010000108         7830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_6642_length_7830_cov_14.916013, whole genome shotgun sequence.
ACCESSION   DAORYL010000108 DAORYL010000000
VERSION     DAORYL010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7830
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6642_length_7830_cov_14.916013"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..1255
                     /gene="ftsZ"
                     /locus_tag="PLV81_13885"
     CDS             83..1255
                     /gene="ftsZ"
                     /locus_tag="PLV81_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOM11228.1"
                     /translation="MFRFEEEPQMNTVIKVVGVGGAGNNAVNRMIATGLEGVDYIVVN
                     TDAQQLKASLAQNKIQIGGKLTRGLGAGADPSIGREAALEDRDKLEKALKGADMVFIT
                     AGMGGGTGTGAAPIVAEVAKECGALVVGVVTKPFRVEGRRRMQHAEEGIKNLKEKVDT
                     IIVIPNDLLLKIIDRNTPIDQAFKLADDILRQGVQGIADIIMVTGLVNVDFADVRTVM
                     KETGDAIMGVGIGVGENRAIEATQMAINSPLLEESGIDGAKAVLLNIAGGNDLSLHEV
                     NEVTEIINKQVDPDANIIFGATIDPALDDKIRVTVIATGFDRKLNMLNRPNELDRATG
                     TPLTVIEGRNNKERMEKKVPVQMKSFSLDYEKRRVKDYSHEDLDIPAFLRRSAESQ"
     gene            complement(1256..2191)
                     /locus_tag="PLV81_13890"
     CDS             complement(1256..2191)
                     /locus_tag="PLV81_13890"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM11229.1"
                     /translation="MNTILLVDHTDYKHEIHKYLSQNYTVLMADSAYDAITTLNMQDV
                     DLVISQVELPGDNAFDLYNYLQKHYTYIPAIMITEKNMDTFFDKIFIQGIGNVLKIPV
                     NQNEFTNLVTKLITRKNIFGLENYVANIINTNAIRINASLQIQPAVEKILATIESWGF
                     TIKNKSVLNLILNEMIINAVYHSHGYTREKEMRIPIQLKEGQFVDITFCHNARTYAIA
                     ITDYQGTLTKEKILHSIHKAIEQEQLILKAAETGEDISDKISETGRGIDLMRKLASEF
                     YFIIQQKKRTEIILLFESDPNEKKSSSLKIIEDYQ"
     gene            2362..2832
                     /locus_tag="PLV81_13895"
     CDS             2362..2832
                     /locus_tag="PLV81_13895"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOM11230.1"
                     /translation="MKKIMLLTLCIIFSFVIISDGGMAQDKEKKSAKEYIADLSSSDE
                     VTVVAAEEWIADNKEKSALPKLHELLRSDNRVKVRLYAAIALGEIANEESAESLNNAL
                     LNDPSADVRYSALLGIARIGSKSSYDAIQKARESEQDPFIKDLIKKMEDKFKGK"
     gene            2914..3699
                     /locus_tag="PLV81_13900"
     CDS             2914..3699
                     /locus_tag="PLV81_13900"
                     /inference="COORDINATES: protein motif:HMM:NF021613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOM11231.1"
                     /translation="MESKVKYVVFDIESVPDANLIKQVKYPHLSISEHDAVIKFQEEI
                     LNVSDGSTWFIPVTFQLPVIVTMLEVDEKFFPVKMRTLDEPHFRTEQIVRQFWDVIEG
                     EYNDASFVTFNGRGFDFPLMELMAFRFGVTAKRHFKDKFASRFRFGTKHIDLHDWLSN
                     YSAIKMQGGLNLLAKVIGKPGKVEMSGNDVYDLYKAGKIEEIHEYCISDVLDTYFVFL
                     RTRVMVGELSYERENAIVQQTRVFLEQNKSNFKGFEQYLKNWK"
     gene            complement(3703..5661)
                     /locus_tag="PLV81_13905"
     CDS             complement(3703..5661)
                     /locus_tag="PLV81_13905"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOM11232.1"
                     /translation="MKELLNFSILTLLCVALLSCNTNVSIKLRVNQGIIDLRNIEDTI
                     ANPIRLDGTWKFYWNYLIESKEQLKLQNPPIDYITVPAPWNKQKNFKSYPAHGYGTYH
                     ITLLHLKKTIGSIMYIKMPFTHTSYAIFINGIPIARSGFVNSNKETMIPNQLPSIIQF
                     LVTSPTTEIFIQVANFQQRNGGILHPVYYGTPKIIESFHNNSIYIQLFVFASLSVIGI
                     YHLGLYLINRSKQNIFIGLFSLIVALRILVINEMLLISFFNPPWSINFRLSYLTVTIT
                     PILFCHFVNSVYPDKIKKYTILTTDLYFGFFTLFVCTAPLHILSSSLVFIQLALVYIS
                     IRLLIILIKETSKNLKTKRIFYAGLILGFACLINDILFSHQIIKTINSSHFGILIFVV
                     TQSMIAFEEYIIQQQRLVALSHEMNIAKKIQRNILPSEPPRLSGISVDIVYLPVTTIG
                     GDFYHFFEIDNKHTGIFIADVTGHGIPASMIASTVNIAFTLQYNHAAHPDIVFSNIND
                     LLIGKTGNQPITALYCYLDIDDMIVRLSRAGHPYPLYFNSKMGTVEEIKTQGNVMGIW
                     PSSDFHTAQFTIYNGDKIILYTDGLIEIRNKHNHQYNLDKLKKSIIENKHLKGSEFTN
                     TIINEVLQWAGSKENITDDITVITIDVG"
     gene            complement(5811..7472)
                     /gene="ilvD"
                     /locus_tag="PLV81_13910"
     CDS             complement(5811..7472)
                     /gene="ilvD"
                     /locus_tag="PLV81_13910"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOM11233.1"
                     /translation="MISDRVKKGIEKAPHRSLFKALGLNDDELAKPLIGVVNSFNEII
                     PGHIHLRNIVDAVKKGVIAAGGTPIEFPTIGVCDGIAMNHIGMKYSLGSRELIADSIE
                     IMAKGHAFDGLVMVTNCDKIVPGMIIAAVKLDIPAIIVSGGPMLPGMYKGKPIAVDNV
                     FEAVGKAAKGTITKEELYEIECAACPGAGSCAGLFTANSMNCLTEALGLALPYNGTIP
                     AVFAERIRLAKESGKKIIELVKKQITPRKIVTKKAIENALAVDMAIGGSSNTALHIPA
                     IAHYAGIPIRLKDIQPISERTPHLCSIAPSGPHHMIDLHYAGGIPALMNELLKHGLIH
                     GTCMTVTGKSIAQNVKDKSILDTSVIRLVTKPVHKDGGLAVLTGNLAPDGCIVKQAAV
                     DPSMLKHSGPAKVFDSEEEAVKAIMGNQIQKGDVIVIRYEGPRGGPGMREMLTPTSVI
                     AGMGMDKEVALITDGRFSGATRGSSIGHISPEAAAGGPLAIVKDGDIIEIDIPAKKLT
                     LKVNDNEIKARLAKWKAPKPKITTGYMYRYSLMVSSADRGAVFES"
     gene            complement(7569..>7830)
                     /locus_tag="PLV81_13915"
     CDS             complement(7569..>7830)
                     /locus_tag="PLV81_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOM11234.1"
                     /translation="KSKPEEDHIVLCIGLGYGKMLRIGDEDVYGAEVNAASKLGEDTA
                     GPWEVLVTNSVKENAKGISNITFEHIDFIPPGADAAYRVIFQ"
BASE COUNT         2495 a         1301 c         1544 g         2490 t
ORIGIN      
        1 atttataata cagtcgttgc agttgaattt tttattaatt ttttttaaaa aataatgcga
       61 cataattaaa aaggaggaaa taatgtttag atttgaagaa gaaccacaaa tgaatactgt
      121 tatcaaggtt gttggcgttg gaggtgcagg caacaatgca gtaaatagga tgattgcaac
      181 aggccttgaa ggagtggatt atattgttgt gaacacggat gcccagcaat tgaaggcatc
      241 gctggctcaa aacaaaatcc agataggtgg taagctgacc agaggattgg gagcaggtgc
      301 tgatccatca attggtaggg aagctgcact tgaagacaga gacaaactgg aaaaggcctt
      361 gaaaggggct gatatggtat tcatcactgc tggaatgggt ggtggtaccg gaacaggcgc
      421 tgcgccaatt gttgccgaag tggcaaaaga atgtggtgcg ctggttgtag gtgttgtcac
      481 aaagccattc cgtgttgaag gccgaagaag aatgcagcat gcagaggaag ggattaaaaa
      541 tcttaaagaa aaagttgata caataattgt gatccccaat gaccttctgt taaagataat
      601 tgatcgtaat acacctatag atcaggcatt taaactggca gatgatattt tgcggcaggg
      661 tgtgcaggga attgctgata tcatcatggt taccggcctt gtcaatgttg attttgctga
      721 tgtcagaacc gtgatgaagg aaaccggtga tgcaataatg ggtgttggta ttggtgttgg
      781 tgaaaacaga gcaatagaag caacgcagat ggcaataaac agcccactgc ttgaagagtc
      841 aggtattgat ggggcaaagg cagtgctgct caacattgct ggtggcaatg atttatcatt
      901 acatgaagtt aatgaagtaa cagaaattat taataagcag gttgacccgg atgcaaatat
      961 catttttggg gcaactatcg acccggcact tgatgacaaa ataagggtaa cagtgattgc
     1021 aacagggttt gaccgcaaac ttaatatgct gaatcgtccc aatgaattgg atagggccac
     1081 tggcactcca ttaacagtta ttgaaggcag aaataataag gaaagaatgg agaaaaaagt
     1141 tccagttcag atgaagtctt tttcactgga ttatgaaaaa cggcgggtaa aagactattc
     1201 acatgaggat ctggatatcc cagctttttt gcgccgaagt gccgaatccc agtgattact
     1261 ggtagtcctc aataattttt aatgagcttg atttcttttc attggggtct gattcaaaaa
     1321 gcaggataat ttctgttcgt tttttttgtt gaatgataaa ataaaattca gaagctaatt
     1381 tacgcataag gtcaatcccg cgtccagttt ctgaaatttt atcgctgata tcttcgccgg
     1441 tttctgctgc tttaagtatc agttgctcct gttcaatggc tttatgaata ctgtgtaata
     1501 ttttttcttt agtaagagtt ccctgataat cagttattgc aatagcatat gtcctggcat
     1561 tatgacagaa ggtgatatca acaaattgac cttctttaag ctggatagga attcgcattt
     1621 ccttttcacg ggtataccca tgtgaatggt acactgcatt gataatcatt tcattaagta
     1681 ttaaatttaa aacgctttta tttttaatag taaatcccca tgattctatg gtagccagta
     1741 ttttttctac tgctggctgg atttgaaggg aagcattaat gcgtatggca tttgtattaa
     1801 ttatgttggc tacataattt tccaggccaa aaatattttt tcgtgtgata agtttagtaa
     1861 caagattggt aaattcattt tgattaactg gtatcttcaa tacattacca ataccctgaa
     1921 taaagatttt atcaaaaaag gtatccatat ttttttcagt aatcataatt gctggtatat
     1981 aggtgtagtg cttttgtaaa taattataaa gatcaaaagc attatcgcca ggcaactcaa
     2041 cctgtgaaat taccagatca acatcctgca tatttaatgt tgtaattgcg tcatatgctg
     2101 aatctgccat caaaacggta taattttgtg aaagatattt atgtatttcg tgtttatagt
     2161 ctgtatggtc aactaataat atcgtattca tagaagctca ttactttaaa aaatttacct
     2221 ggcgatagtt cctgtcaata gtaactatcg caaaaattat atatttatat aagaaaatca
     2281 ttgacattct tttcaaaaaa aggatactat acataaaata tactatcgtc aatactatcg
     2341 tcaaaaaatg agaggtgcac aatgaaaaaa ataatgttac taacattatg tattattttt
     2401 tcgtttgtta taatttctga tggaggaatg gcccaggata aggaaaagaa aagtgctaag
     2461 gaatatatag ccgatctttc aagcagtgat gaagtaactg ttgtggctgc agaggagtgg
     2521 attgcagata ataaggaaaa gtcagcactt ccaaaattac acgaacttct gcgcagtgac
     2581 aacagagtga aagtgcgttt atatgcagct atagcgttgg gcgaaattgc caatgaagaa
     2641 agtgcagaaa gcctcaataa tgcattgctt aatgacccca gtgctgatgt ccgctattca
     2701 gcattattag ggatagcacg tataggttca aaaagttcat atgatgctat tcagaaagca
     2761 cgtgaaagtg agcaggatcc attcattaag gacctgatca aaaaaatgga agataagttt
     2821 aaaggaaaat aaattattaa tattagattt tatgtaaaaa gcatgtgggg taatctccac
     2881 atgcattttt attttattat tagggttgta aacgtggaat ccaaagttaa atatgttgtt
     2941 tttgatattg agtcagtgcc tgatgcaaac ttgataaaac aggtgaagta tcctcattta
     3001 tctattagcg aacatgatgc tgttattaaa tttcaggaag aaatattaaa tgtatctgat
     3061 ggatccacgt ggttcattcc cgttacattc cagttgcctg taatagtaac catgcttgaa
     3121 gttgatgaaa agtttttccc agtaaagatg cggactctgg atgagcctca tttcaggacc
     3181 gaacagattg taaggcagtt ctgggatgtt attgaaggtg aatataacga tgcctctttt
     3241 gttacgttca atggtcgggg atttgatttc cccttaatgg aactgatggc ttttagattt
     3301 ggggtaactg ccaaacgaca tttcaaagat aaatttgcat cccgtttcag gtttggcacc
     3361 aagcatattg atctgcacga ctggctatcc aattacagtg caataaagat gcagggtggg
     3421 ttaaacttac ttgcaaaggt aataggtaaa cctggcaagg ttgaaatgag tggtaatgat
     3481 gtgtatgatc tttataaagc aggcaagatt gaagaaattc atgaatactg tatctcagat
     3541 gttcttgaca catactttgt gtttttacga acaagagtaa tggtgggtga actaagctat
     3601 gaaagagaaa atgcaattgt tcagcaaacc agagtttttc tggaacaaaa taaatcaaat
     3661 tttaaaggat ttgagcagta tttaaaaaac tggaaataga tttcaaccta catctattgt
     3721 aattacggtt atatcatctg taatgttttc tttgctacca gcccattgta atacttcatt
     3781 aataatcgtg tttgtaaatt cactgccttt cagatgttta ttttcaatga tggatttttt
     3841 aagcttatcc agattgtatt ggtgattgtg tttatttcgt atttctatta atccatcagt
     3901 ataaagaatt attttatcac cattgtatat agtaaattgt gctgtatgaa aatcagatga
     3961 tggccatata cccatgacgt ttccctgtgt tttaatctct tcaacagtac ccattttaga
     4021 attaaaatat aatggatagg gatggcctgc ccgggataaa cgtactatca tatcgtctat
     4081 atcaagataa cagtataatg ccgtaatagg ctgattacca gttttgccaa tcaaaagatc
     4141 attgatatta ctgaatacaa tatcaggatg tgcagcatga ttatactgta atgtaaaagc
     4201 aatgttaaca gttgatgcta tcatggatgc tggaattcca tgtccagtaa catctgcaat
     4261 aaatattcct gtgtgtttat tgtctatctc aaaaaaatga taaaaatcac caccaatggt
     4321 ggtaaccggt aaatatacaa tgtcaactga gatgccagaa agacgaggtg gttcagatgg
     4381 caaaatattt ctttgtattt tttttgcaat attcatctca tgtgacagtg caaccagcct
     4441 ttgttgttga atgatatatt cttcaaaagc tatcattgat tgagttacaa caaatataag
     4501 aataccaaaa tggctggagt ttattgtctt tattatttga tgcgaaaaca atatatcatt
     4561 tataagacaa gcaaaaccta gaataaggcc agcataaaaa attcgctttg tttttaagtt
     4621 tttgcttgtt tctttaataa gaataatgag aagacggatg gaaatatata caagtgccag
     4681 ttgtatgaat accagggaag aagataagat atgcagagga gcagtacaaa caaataaagt
     4741 gaaaaatcca aagtaaaggt ctgttgtaag tatggtatac tttttaattt tatctggata
     4801 gacgctattt acgaaatggc aaaaaagtat tggtgttatg gttacagtca ggtatgatag
     4861 cctgaaattg atgctccatg gtgggttgaa aaagcttatt agaagcattt cgttgataac
     4921 aagaatgcgt aaagccacaa tcaatgaaaa aagccctatg aaaatatttt gctttgacct
     4981 gttaatcaaa tataacccta aatgatagat acctattaca gacaatgaag caaatacaaa
     5041 aagctgaata tatatggaat tattatgaaa gctctcgata attttaggtg tcccataata
     5101 gacaggatgt aagatgcctc cattacgttg ctgaaaattt gcaacctgta tgaatatttc
     5161 tgtagtaggg gatgttacca gaaattgtat tattgaaggt aactggttag gtatcatggt
     5221 ttctttgttg ctgttaacaa atccactacg tgctattggg attccattaa taaatattgc
     5281 atatgaggtg tgtgtaaagg gcattttgat atacataata gagccaatag ttttttttaa
     5341 atgcaacagc gtaatatgat aggtcccata cccatgggca gggtaggatt tgaaattttt
     5401 ttgcttattc catggtgcag ggactgtaat ataatcaata ggcgggtttt gtaattttaa
     5461 ctgttctttg ctttcaataa gatagttcca gtaaaatttc catgttccat caagcctgat
     5521 aggatttgcg atagtatctt caatatttct aagatcgata attccctgat ttactctaag
     5581 ctttatagaa acattggtat tgcaagaaag caatgcaaca cacaataagg ttaaaataga
     5641 aaaattaaga agctctttca agtatgtcat cataatgtaa gttattttaa agtattagat
     5701 ataaattata attaataaat acaatgtaaa gaaatttttt atacgtgagg aatgttgtgt
     5761 atacggtggg gtgtacaagc gatacacccc actatagggt tatatgcaga ttaactttcg
     5821 aatacagcgc cgcggtcggc ggatgatacc atgaggctat agcggtacat atatccagta
     5881 gtaatctttg gttttggtgc tttccatttg gccagccgtg cttttatttc gttatcattt
     5941 acttttaacg taagtttttt agcagggata tcaatttcaa taatatcacc atctttaaca
     6001 atggcaagtg gaccaccagc agcagcttca ggtgaaatat gaccaataga tgatccacgt
     6061 gttgctccag aaaatctgcc atcagttatc agtgcaacct ctttatccat tcccatacca
     6121 gcaataactg atgttggtgt tagcatttca cgcattccag gccctccccg cggtccttcg
     6181 taacgaataa ctatgacatc acctttctgt atctggtttc ccatgatggc tttaactgct
     6241 tcttcttctg aatcaaagac ttttgcaggc ccactatgtt tcaacataga tgggtcaaca
     6301 gcagcctgtt ttactataca gccatcaggt gccagattcc cggtaagtac tgcaagtccg
     6361 ccatctttat gaacaggttt agtaactaat cgtattactg aagtatccag tattgattta
     6421 tcttttacat tttgtgctat tgatttgcca gtaactgtca tgcaggtgcc atgaataagc
     6481 ccgtgtttaa gcagttcatt cattaatgcc gggataccac cagcataatg caggtcaatc
     6541 atgtggtgtg gtcctgatgg tgcaatgctg cataaatgag gtgtacgctc agaaatgggc
     6601 tgaatgtctt ttagtctgat agggattcct gcataatgag ctattgccgg aatatgcaaa
     6661 gcggtatttg aagagccccc tattgccata tcaacagcaa gtgcattttc aatggctttt
     6721 ttagttacta tcttgcgggg agttatttgc tttttaacaa gttcaattat cttctttcca
     6781 ctttccttgg caaggcgaat acgttcggca aaaactgcag gtattgtgcc attataggga
     6841 agagcaagtc ccaaggcttc agtaagacaa ttcatggaat tagcagtgaa aagccctgca
     6901 caggagcctg cgcccgggca tgctgcacat tctatttcat ataattcttc ttttgtgata
     6961 gttccttttg cagctttgcc aactgcttca aatacattgt caactgcaat aggcttgcct
     7021 ttatacattc caggaagcat tgggccacca gacacaataa ttgctggtat atctaatttc
     7081 acagctgcta taatcatacc ggggacaatt ttatcacaat tggttaccat aacaaggcca
     7141 tcaaacgcat gcccttttgc cataatttca attgagtcgg caataagttc acgtgatcct
     7201 aatgaatatt tcatgccaat gtggttcatg gcaatgccat cacacacacc tattgtaggg
     7261 aattcaatcg gcgtgccacc agcagcaatg acaccttttt taactgcatc tacaatattg
     7321 cgtaaatgta tatgtcccgg aattatctca ttaaatgagt taacaacgcc aattaatggt
     7381 tttgctaatt cgtcatcatt taaccctaac gctttaaaca gtgagcgatg aggagctttc
     7441 tcaattcctt tcttaacgcg atcactgatc ataaaacatt cctccttaaa aaaatgaaca
     7501 atataaaaag tgattattaa attgacactt tgaaattgta aattaaaatt ttaaaaaagt
     7561 cattcaaatc attgaaaaat aaccctatat gctgcatcag cacccggtgg gataaaatca
     7621 atatgttcaa atgttatatt agagatccct ttagcatttt cttttactga gtttgtcaca
     7681 agaacttccc atggtcctgc tgtatcttca cctaatttac ttgctgcgtt cacttcggct
     7741 ccataaacat cttcgtcacc aatacgcaac attttcccat atcccagacc aatacataat
     7801 acaatatgat cttcttcagg tttacttttg
//
LOCUS       DAORYL010000109         7806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_6666_length_7806_cov_6.147594, whole genome shotgun sequence.
ACCESSION   DAORYL010000109 DAORYL010000000
VERSION     DAORYL010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6666_length_7806_cov_6.147594"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /gene="cas6"
                     /locus_tag="PLV81_13920"
     CDS             <1..763
                     /gene="cas6"
                     /locus_tag="PLV81_13920"
                     /inference="COORDINATES: protein motif:HMM:NF021551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR system precrRNA processing
                     endoribonuclease RAMP protein Cas6"
                     /protein_id="HOM11235.1"
                     /translation="QFILHCPYASIFESHNVGDIEVMKEATNFPHPFVMSPLVQWPST
                     IKPGESFTVYLSIFGEAIKYFHFFVKALKALGQRGLGKEKSKFVVTEITNYHNGDKLY
                     TANEGYVGENIEGLYINHDVVTGLQINFLTPCHIKYNGAIITEPTLNVIIKNILRRYK
                     IISNIYGDSSVIYTEDDINYDSIKVVDVHTEWYSTDRLSKRQNRIMKIEGFTGSMNVQ
                     GDISALYNILKIGEAIHLGKHTSFGCGLMKVNSLN"
     gene            787..3204
                     /gene="cas10"
                     /locus_tag="PLV81_13925"
     CDS             787..3204
                     /gene="cas10"
                     /locus_tag="PLV81_13925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Cas10/Csm1"
                     /protein_id="HOM11236.1"
                     /translation="MDNSLKNQYHMLLGALFHDIGKFKQRAYGGKPHSQFPQSMDSFI
                     LPSTQSGYGYMHALWTFDFFESDLKTLQLPNDLDWNSIRDLAAKHHKPSTFEEEILQK
                     ADRLSSSIDRTENDEHFTSGDYLKKHLRSIFTQISVDNEPTKDVYGYNIATLEIDNSF
                     PLIIKQETESLEDNYNKLWNDFITLYKKMCLQWGKNNTIHHFVHSTAHLLKKFTWCIP
                     AATNDKLCDSSLYDHSVSTASIALSLLQYCKDKKMPSDNEKAFLLFAADISGIQQFIF
                     QRHHQIFKGSSKIIRGRSFFVSSIGLAYQYLLYQKLGIIPFTQLLSSSGKFYLLLPNT
                     QKVQETISEVVEQVDRWLFKEFHGDFAIVTDYSINASINDLSNSFADLIGTINYNLSV
                     SKFNKFRNILKQGECVIDVNYTEDEICPSCGKYTKEKQYKDEEGERCEFCNTMFEIGA
                     KIVRNPLYIVIQNNNHGDVNFFSNNISITFANDALPYTDALALYTLDTIKADTCMAWE
                     FNNYVPIANDEIKTFEDIAQSSIIHYKDDDPDTKRGVPLLSFIKLDVDNLGFIFSHGI
                     HNLSIARYVTVSRMINYYFNSVVYSILHKKFPNVYTVLSGGDDLFLVAPFRDTIPLIK
                     EIYNQFKNFTCTNADIHFSTGVFVCHDNYPMSKAAEMAQEELEKAKSSSEFKNKVAYF
                     TVMDYDKQLFLITEHVKWFEQKILQKDSPVSQAFIYRLLMYTRQSHSNTFEKYLYIPK
                     FKYDVARNIVKKENGKILNPEEITWIENFFENNVAKNSAWVEAVLITAIYSMRKYQDK
                     SKEVMSA"
     gene            3201..3596
                     /gene="csm2"
                     /locus_tag="PLV81_13930"
     CDS             3201..3596
                     /gene="csm2"
                     /locus_tag="PLV81_13930"
                     /inference="COORDINATES: protein motif:HMM:NF015695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Csm2"
                     /protein_id="HOM11237.1"
                     /translation="MIQITPEYLTTQAEEKAKLFKERGLESSQMRKYYDELKLQEKRA
                     YEIASKNDEFKTKVLPFVKFVKAKIAYGVGRTVSGKKLVPEEFKQHMDAQINRIESFE
                     DLRNFLLHFQAIICYFTYYKERQQDGGRR"
     gene            3593..4234
                     /gene="csm3"
                     /locus_tag="PLV81_13935"
     CDS             3593..4234
                     /gene="csm3"
                     /locus_tag="PLV81_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm3"
                     /protein_id="HOM11238.1"
                     /translation="MNKLIDTKILTGKIVLETGLHIGASTKGVGIGEIDNPVIKDPRT
                     GYPYIPGSSLKGKIRSLLELKYGINSSNKEKPCECGNCDVCKVFGTTAASSKAGITRV
                     LFRDAFLSEESKKELHEKNLLPTEEKTENMINRLGGYAESPRTTERVIPGLKFDFEVA
                     IRVFDGDNDLSTKLLKEGLKRLQKDALGGSISRGYGKVRFENLRLDNQEFTLE"
     gene            4238..5200
                     /gene="csm4"
                     /locus_tag="PLV81_13940"
     CDS             4238..5200
                     /gene="csm4"
                     /locus_tag="PLV81_13940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm4"
                     /protein_id="HOM11239.1"
                     /translation="MAAIYKIVCKPHSHFEMVYKADTIFGALCWAVRYIYGEQKLVDM
                     LEQFKQLPPFLLSSMMPMGYLYKPLLHPEKIDDTVNQHNGTKQSKKQLKQKIEQEKRK
                     KRITIIPLTLFQKYQKNFKDIYQDPELLKTSLFEAGLGSHEITRNAIDRISMHAREGV
                     LFTELYMYSQKPLVFYIKVLDSSYSKEWFEKVVELIGKNGLGKDCSVGKGIFAMSFDN
                     LTKEEESIFTYTSNTFCTLSVCAGSNCNPLMYTTFTRYGKLGGAYSQNGIDGKLLFCK
                     KPVVFYGEGSVFNGNGIAGSIISNIHSNSSIVQYGYAFPVYFTS"
     gene            5213..6334
                     /gene="csm5"
                     /locus_tag="PLV81_13945"
     CDS             5213..6334
                     /gene="csm5"
                     /locus_tag="PLV81_13945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm5"
                     /protein_id="HOM11240.1"
                     /translation="MLQYNAIIRIITPIIIQTGQQYDLWDLMPNPKNPKEIWIINPSL
                     AYASLSQEMQKKFDLLIDELSKLKGDAVKQKMTALRDMLHGAVISNPSVIMQKAQGLD
                     NFKEELTRNPNAEIRKIYKESLTNRPYIPGSSIKGMIRTAVLEAIRKNKQLIPNIDRD
                     HDVRRGKVSIKAVHSFEDRILLGANRRKVYDDPFKFLHVSDFIIEKTDTIFGTVRVTS
                     ARGKIPTYTEMTGCELLNNQEYLAKGTITIYDDELKKYLATLNQYDKSALHILPQVFR
                     AEFILGALKNFYEPLLNNKKHPVDSEIKNLMQNKNKSNTAVPIRLGRFSQVESKTFKV
                     ERTWERGDSRRMNFEGGKTRAYVKGTIGAGWGLMTLTKA"
     gene            complement(6396..7787)
                     /gene="csx1"
                     /locus_tag="PLV81_13950"
     CDS             complement(6396..7787)
                     /gene="csx1"
                     /locus_tag="PLV81_13950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated CARF protein Csx1"
                     /protein_id="HOM11241.1"
                     /translation="MKLLIEIWGNYHTWKEVTYTYNDSTITSTTTIPLLLQTIKPDKS
                     LIIVADTLLEYEIANNTLATELNNTTNWYRDAIKAVEHSTKQFIINALDNTKTKASTD
                     TISVLVTPAVGTFNKTKFIGNPNNFFSYLYYIMAKAAVTIAHNSNDKELEIYVDITHG
                     MNYMTMITYRTIKDICSILAYFFDITFVVLNSDPFVGSDNIHLNINQIEKVKIKPQFN
                     FFRYPQFDTGQYLRVSQKIDNDKKALINKKLQNIIKEQLEGYKPFIEDYYCFASSVYH
                     ALPLYLVYFYPDITKLKTIIDIIVEIFFNHFMYITDSKFIIQQQVDFTPSFLSLLQLA
                     IFAQCLQSKYNITRQAECPLPLLNDILQIYIANPTITSRIYRELDKLQLVVHELQVIP
                     ENYVDYGLLLKKSDYIPSTNVDNRNFFAHSGFGYNNTLIKNVNKTIYIKPKEESLQEI
                     KKVLIDNLPLGVM"
BASE COUNT         2804 a         1067 c         1429 g         2506 t
ORIGIN      
        1 tcagtttatt cttcattgcc cgtatgcatc tatatttgaa tcgcataatg tgggtgatat
       61 agaagtaatg aaagaagcaa caaacttccc tcatccattt gtcatgtcac ctttagtaca
      121 gtggccaagt accattaagc caggcgagtc atttactgta tatctttcaa tttttggtga
      181 ggcaataaag tattttcact ttttcgtcaa agcattgaaa gcattgggcc aacgagggtt
      241 aggtaaagaa aaatcaaaat ttgttgtaac agaaattact aattaccata atggggataa
      301 actatatacg gctaatgaag ggtatgttgg ggaaaatatt gaaggtttat atataaacca
      361 tgatgtggta acgggacttc aaataaactt tttaacaccc tgccatataa aatataatgg
      421 cgcaataatt acagagccca cattaaatgt aataataaaa aatattttac gtagatataa
      481 aataatttcc aatatttatg gggattcttc agtcatatat actgaggatg atattaatta
      541 tgattcaatt aaggttgtgg atgtacatac tgaatggtat tctaccgaca gattgtcaaa
      601 acgccagaac agaattatga aaatagaagg ttttactgga agtatgaatg tgcagggtga
      661 tattagtgct ttatataata tactaaaaat tggggaagct atacatttgg ggaaacacac
      721 atcgtttgga tgtggtttga tgaaggtaaa ttcattaaat taacttttta attggagtaa
      781 tagtccatgg ataattcatt gaaaaatcag tatcacatgt tattgggagc attattccat
      841 gatattggaa aatttaaaca acgagcatat ggaggaaagc ctcactctca attcccacaa
      901 tcaatggatt cttttatttt gccatctaca caaagtggtt atggatatat gcatgcatta
      961 tggacttttg atttttttga aagtgactta aaaactttac aattaccaaa tgatcttgac
     1021 tggaattcaa tccgtgattt agctgcaaaa caccataagc cttcaacgtt tgaagaagaa
     1081 atattgcaaa aagctgatag actgtcttca agtattgacc gtacagaaaa tgatgaacat
     1141 tttacaagtg gtgattattt aaaaaagcat ttacggtcaa tatttactca gatatcagta
     1201 gataacgagc caacaaaaga tgtatatggc tataatattg ctacattaga aatagataac
     1261 tcattcccat taataataaa gcaagaaact gagtcattgg aagataacta taataaacta
     1321 tggaatgatt ttattacttt gtataaaaag atgtgcctgc agtggggcaa aaacaataca
     1381 atacatcatt ttgtccattc aactgcacat ttattgaaaa aatttacctg gtgcattcct
     1441 gcagcaacca atgacaagct gtgtgacagt tcactctatg atcattccgt tagtactgct
     1501 tctattgcat tatctctatt acagtattgt aaagataaaa agatgccttc cgataatgaa
     1561 aaagcatttt tattatttgc agccgatatt tcaggaattc agcaatttat ttttcagcgt
     1621 catcatcaaa tatttaaagg tagttccaaa attattagag gtaggtcatt ctttgtatca
     1681 tctattggtc tggcatatca ataccttttg tatcaaaaat taggcataat tccttttacg
     1741 caattattat catccagtgg taaattttat ttacttctgc ccaatacgca aaaagtacaa
     1801 gaaacaatta gtgaagtagt tgagcaggtt gacaggtggc tttttaaaga atttcatggg
     1861 gattttgcga tagttactga ttattcaatt aatgcctcaa ttaatgattt aagcaattca
     1921 tttgctgatt taattgggac tattaattac aacctttcag tatcaaagtt taataaattt
     1981 agaaatatat taaaacaagg tgaatgtgtt atagatgtca actatacgga agatgaaata
     2041 tgcccttcat gtggtaaata cacaaaagaa aagcaataca aggatgaaga aggtgaacga
     2101 tgtgaatttt gcaatactat gtttgagata ggtgccaaga ttgtacgaaa tccattatac
     2161 attgttattc aaaataacaa tcatggtgat gttaactttt ttagcaataa tataagtatt
     2221 acctttgcta atgatgcact gccttatact gatgcattgg cgttatatac tcttgacacg
     2281 attaaagctg atacctgtat ggcatgggag tttaataatt atgttcctat agcaaatgat
     2341 gaaataaaga cgtttgagga tattgcacaa tcttcaataa ttcattataa ggatgatgat
     2401 cccgatacaa agcgcggtgt gccattatta tcattcataa agttggatgt tgataatctg
     2461 ggttttatat tttcacatgg aatacacaat ttgagcattg cacggtatgt aactgtatca
     2521 aggatgataa attattattt taactcagta gtatattcaa tattgcataa aaaattccca
     2581 aatgtttata cagtgttatc tggtggcgat gatttgtttt tagtagcgcc attcagagat
     2641 actattccat taattaaaga aatatataat caatttaaaa attttacctg tactaatgct
     2701 gatatacact tttccacagg tgtatttgtg tgtcatgata attatccaat gagtaaagca
     2761 gcggaaatgg cccaggaaga attagaaaag gcaaagagca gcagtgaatt taaaaacaaa
     2821 gtagcctatt ttaccgtaat ggattatgat aaacaactat ttttaataac agaacatgta
     2881 aaatggtttg aacaaaaaat tttgcaaaag gattcacctg ttagtcaggc gtttatttac
     2941 aggctattga tgtatacacg ccagtcacat tctaatactt ttgaaaaata tttatatatt
     3001 cccaaattta aatatgatgt agcacgtaat atagtcaaaa aagaaaatgg taaaatttta
     3061 aatccagaag agatcacatg gattgagaac ttctttgaaa ataatgtagc aaaaaattca
     3121 gcatgggtag aggcagtgct tattactgct atttactcta tgcgtaaata tcaagataaa
     3181 agcaaggagg taatgagcgc atgatacaaa taactcccga atatttaaca actcaagctg
     3241 aagaaaaagc taaattattt aaggaaagag ggcttgaatc atcgcaaatg cgaaaatatt
     3301 acgatgaatt gaagctgcag gaaaaacgag cttatgaaat tgcatccaaa aatgatgaat
     3361 ttaaaaccaa agtgttgcca tttgtaaaat ttgtaaaagc taaaattgcg tacggtgttg
     3421 gtcgtactgt aagtggtaag aaattggtac ctgaagagtt taaacaacat atggatgccc
     3481 aaataaatag aattgaaagt tttgaggatc ttagaaattt cttattacat ttccaggcta
     3541 taatatgtta ttttacatat tataaagaaa gacaacagga cggaggtaga cgatgaataa
     3601 actaattgat actaaaattt taactggtaa aatagttctt gaaactggtc ttcatattgg
     3661 agctagcaca aaaggtgttg gtattggcga aattgacaat ccagtcatta aagatccacg
     3721 aacaggatat ccctatatac cagggtcttc attaaaaggt aagatacgtt cattgctgga
     3781 acttaaatac ggtattaatt caagtaataa agaaaaaccg tgtgaatgcg gtaattgcga
     3841 tgtatgtaaa gtttttggaa ctacagcagc ttcaagtaag gctggtataa cgcgagtgtt
     3901 attcagagat gcctttttgt ctgaagaatc aaaaaaagaa ttacatgaaa aaaatttact
     3961 gccaactgaa gaaaaaacag aaaatatgat taacagatta ggtggttatg cagaaagtcc
     4021 tcgtacaact gagcgagtta ttcctggctt aaaatttgat tttgaagtag ctattagagt
     4081 ttttgacggt gacaatgatt tatctacaaa acttttgaaa gaaggactaa aacgtttaca
     4141 aaaagatgct cttggtggta gtataagcag gggatatggt aaagtacgtt ttgaaaattt
     4201 gcggttggac aatcaagaat ttacattgga gtaatatatg gctgcaattt ataaaatagt
     4261 gtgcaagcca cactcgcact ttgaaatggt ttataaggct gacaccatat ttggtgcatt
     4321 gtgctgggcc gtacgttata tttatggtga gcaaaagtta gttgatatgc tggagcagtt
     4381 taaacaattg ccaccatttt tgctttcatc catgatgcca atgggatatt tatataaacc
     4441 tttacttcat cctgaaaaaa ttgatgacac agtaaaccaa cacaatggta caaagcaatc
     4501 taaaaaacaa ttaaaacaaa agattgaaca ggaaaaaagg aaaaagcgaa ttacaataat
     4561 tccattaacg ctatttcaaa aatatcaaaa gaattttaaa gatatatatc aagaccctga
     4621 gcttttaaaa acctcattat ttgaagcagg gctgggtagt catgaaataa caagaaatgc
     4681 tattgataga atatcaatgc atgcccgtga gggagtatta ttcactgaat tgtatatgta
     4741 cagtcaaaaa cctttagtgt tttatataaa ggtattggat tcttcatatt caaaagaatg
     4801 gtttgaaaaa gtagtggaac taattggaaa aaatggactt ggtaaggatt gcagtgttgg
     4861 caaaggtata tttgcaatga gttttgataa tttgacaaag gaagaagaaa gtatttttac
     4921 gtataccagc aatactttct gtacattaag tgtttgtgca ggttctaact gcaatccatt
     4981 gatgtataca actttcaccc gctatggaaa acttggtggg gcatatagcc agaatgggat
     5041 agatggtaag cttttgtttt gtaagaaacc tgtagtgttt tatggtgaag gttcagtttt
     5101 caatggtaat ggtatagctg gtagtataat tagcaatatt catagtaatt ccagcatagt
     5161 acaatatggg tatgcattcc cagtatattt taccagttaa gggagaatta caatgttgca
     5221 atataatgcc ataatacgga taattacacc aattatcata caaactggac aacagtatga
     5281 tttatgggat ttaatgccaa atccaaaaaa tccaaaagaa atttggatta ttaatccatc
     5341 attggcatat gcttcattaa gtcaggaaat gcaaaaaaaa tttgacttgt taattgatga
     5401 gctttcaaaa ctgaaaggag atgctgttaa acaaaaaatg acagcattgc gtgatatgtt
     5461 gcatggtgca gtaatcagta atccgtctgt tattatgcaa aaggctcaag ggcttgataa
     5521 ctttaaagaa gaattaacca gaaatccaaa cgcggaaata agaaaaattt ataaagaatc
     5581 attaacaaac cgaccctata taccaggttc ctcaattaag gggatgatac gaactgcagt
     5641 attagaagcc attagaaaaa ataaacaatt aatacccaac atagatagag atcatgatgt
     5701 cagacgaggt aaagtaagta ttaaagcagt acattcattt gaagatagaa tattgcttgg
     5761 tgctaataga agaaaggttt atgatgatcc atttaaattt ttacatgttt cggattttat
     5821 tatagaaaaa actgatacaa tatttggaac ggtcagggta acatcagcaa gggggaaaat
     5881 accaacatat actgaaatga caggatgtga gttattaaat aatcaggaat atttagcaaa
     5941 aggtacaatt acaatatatg atgatgaatt aaaaaaatat ttggcaacgt taaaccaata
     6001 cgataaaagt gctttacaca tactaccaca agtattcaga gcagagttta ttttgggtgc
     6061 tttgaaaaat ttttatgaac ctttgcttaa taataaaaaa catcctgttg atagcgaaat
     6121 taagaattta atgcagaata aaaataaatc taacacagca gttcctatac gtttagggcg
     6181 ttttagccag gtggaatcaa aaacttttaa agttgaacgt acatgggaac gtggtgattc
     6241 tagacgaatg aattttgaag ggggaaaaac gcgtgcttat gtaaaaggca ccataggagc
     6301 tggctgggga ttaatgacat taacaaaagc atagtaaaat tatatgtaaa ttaggtgttc
     6361 taaataaaaa aacaacatga ttaattttat agtaatcaca tcacacctaa tggtagattg
     6421 tctataagta cttttttgat ttcttgtagc gattcttctt ttggtttaat atagattgtt
     6481 ttgtttacat tcttaatcag tgtgttattg tacccaaaac cagagtgagc aaagaaatta
     6541 cgattatcaa cattagttga tgggatataa tcacttttct taagcagtaa gccataatcc
     6601 acatagtttt ctggaataac ttgcagttca tgtactacta attgtaattt atcaagttcc
     6661 ctatagatgc gtgaagtaat agttgggttg gctatatata tttgtaaaat atcatttaac
     6721 aatggtagcg gacattcagc ctggcgggtg atgttatatt tagactgtaa gcattgtgca
     6781 aagatagcta attgtaatag cgatagaaaa gaaggtgtaa aatcaacctg ctgctgaatg
     6841 ataaatttgg aatcagttat gtacataaaa tgattaaaga atatttcaac tataatgtct
     6901 attattgttt ttagtttagt gatatctggg taaaaatata caaggtaaag tggtagtgca
     6961 tgatacactg aacttgcaaa gcagtaatag tcttcaataa atggtttgta gccttcaagc
     7021 tgttctttta tgatattctg aagttttttg ttaattaatg cctttttatc attgtcaatt
     7081 ttttgtgaaa ctcttaggta ttgtcctgta tcaaattgcg gatatctaaa aaagttaaac
     7141 tgtggtttaa ttttaacttt ttcaatttga ttaatattta aatgaatatt gtcagaacca
     7201 acaaagggat cactgttcaa tacaacaaaa gtaatatcaa aaaaatatgc cagtattgaa
     7261 caaatgtctt ttatagtacg gtatgttatc attgtcatgt aattcattcc atgggtaata
     7321 tctacgtaaa tttctaattc tttatcgtta gaattgtggg caatagtaac tgcagccttt
     7381 gccatgatgt aatataagta agaaaagaaa ttatttgggt tcccaataaa cttcgtttta
     7441 ttaaatgtac caacggcagg agtaaccagc actgatatag tatccgttga agcttttgtt
     7501 ttcgtattat ccaatgcatt gattataaat tgctttgttg aatgttctac tgctttgata
     7561 gcatcacgat accagtttgt ggtgttattt agttctgtag ctaaagtgtt attggctatt
     7621 tcgtattcaa gcaaggtatc agcaactata ataagacttt tatcaggttt aatggtttgt
     7681 aaaagcagag ggattgtggt agttgaagtt attgttgaat cattatacgt gtaagtaact
     7741 tctttccagg tgtgataatt tccccaaatc tcaatgagta gcttcattgc tgtctccagt
     7801 agttaa
//
LOCUS       DAORYL010000110         7499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_6995_length_7499_cov_12.988313, whole genome shotgun sequence.
ACCESSION   DAORYL010000110 DAORYL010000000
VERSION     DAORYL010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7499
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6995_length_7499_cov_12.988313"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..892)
                     /locus_tag="PLV81_13955"
     CDS             complement(47..892)
                     /locus_tag="PLV81_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11242.1"
                     /translation="MKTRKSFTIDEIAALKPNEKIALVATINNEGLPHVTLLTSLLAL
                     DDTHMALGEFSKGVSKEYIKYNNNIGFCILTMNMDMWRGKARYTHSAKEGPEYEMFNE
                     FPMFRYNTYFGINTVHYFDLLEANEKERLPLAAIAKAVIATLCSKWAAKQKKDTRPLD
                     TPGFELFNRLDALKFAAWIDSDGFPKIVPVLQAKASDSSTIVFSPLAFREELLQLKEG
                     TPVAVYALTVKMENILTRGIFKGYRRYAGITLGMCDINWVYNSMPPVHGQIYPRVPLT
                     PVKDF"
     gene            complement(915..1928)
                     /locus_tag="PLV81_13960"
     CDS             complement(915..1928)
                     /locus_tag="PLV81_13960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOM11243.1"
                     /translation="MTIDSTGIVLEIQRMSTEDGPGIRTTVFMKGCPLKCVWCHNPES
                     INPKPQVQWIGARCIGCKTCVQACPNGALTFDDGIHINRTVCKGCGRCTEVCPSTAME
                     LLGKQWSADALVQELLKDNAYFGTSGGGVTISGGESTFQAHFAKKVLVELKHHGIHTA
                     IDTCGICNQHTLDMLLPHVDLVLYDLKEMDPLKHQQFTGKSNYRVLNNIKYILSFMKE
                     HLFPREVWIRTPLIPGKTARDENIVAIAGEINNLLSINPKAITRWDLCAFNNLCKDKY
                     TRLGLEWELAHTPLLTKEELSHFEALAKQHCNNPAIVKTSGSTRIEDDEAPVTKPKKK
                     LGC"
     gene            complement(1938..4439)
                     /locus_tag="PLV81_13965"
     CDS             complement(1938..4439)
                     /locus_tag="PLV81_13965"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOM11244.1"
                     /translation="MSQTSPKAQLKQPKNLSPRIQWLRDYYFKGAERKWNNEYKSFTT
                     GTPWDAQFNEMTFYIVPETYTLMQTMYQSFRMAAHKVDVPKDFYSWSIPERRAWFNKE
                     VMVNYLPHEILPGDLIAGARFNIQTSFCLTKSEMKKRIRLVMGKNGARAKMKWFHDHG
                     FGNAGATSGHLIPDYQKVLQKGFKGIYAEIEGFYNELSPKEKKGNKGAVLRAMMTAAT
                     MPRDVALQYAKECKRLAKEESNSARREELEQMAANLERVPWEPAVSFWEAVQSLWLAH
                     MLVMSDENYPGPGVSFGRIDQYLYPYWQYSLTKGMDREFGKEILKCFFIHANTAYDAM
                     IRTGNNGITSGYGQLITLAGLGKDGNDMANDLTFAFLEVIDEMSPILEPKPNIRLHQK
                     SPDALLDKVVDMIASCQGAPFLLNFDERSMAGMIREAKKAGIEHLINENNVYDYAPVG
                     CLENTMVGNDRSGTVDNNLNLAKAIELALNNGKDFFPFTDPVTGKVEPVKQIGPRTGD
                     PRTFNTFDEFWNAYEIQTKFIIKKIVELYEESERIRAQYHPTPYLSCLVKGCAEKGKD
                     ITQGAAEISFTTLEAVTYATTVDSLLAIKYMVYDKKVCTMDELIQALKANWEGYEYLQ
                     SIAKNRAPKYGRDDDEADAMGLKVMQLWTEETWKHKTKSTGRQFRPGMLSWNYWVGDG
                     YILPATPDGRKKGQFLSNAICPVNGADINGPTANANSVGKVLGGRDTPGDFGGYVNLL
                     PNGASHTITFNPSILKDPEHRGKFKAFLRGYAINGGTALQINMLDPDMLRDAQKHPQD
                     YRHLLVRVTGYNAYFVSIGKELQDEVIARVSHNKF"
     gene            complement(4436..5173)
                     /locus_tag="PLV81_13970"
     CDS             complement(4436..5173)
                     /locus_tag="PLV81_13970"
                     /inference="COORDINATES: protein motif:HMM:NF019456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HOM11245.1"
                     /translation="MYLEPLKADSLVEAFVKRFEELIISGKLTIGQKIPSERELAKQL
                     GVSRPVVHEGLIILEQKGLVTIRPRHGVTVNDYRREGSLAILQSLVSYATGQFEPHIL
                     ESLLATRTLIELETVKLAAQHRTQQQLDEFRAIITEEQSLIPAQTQAIVDVDFKFHHL
                     IALASGNVVYPLLLNSFRQVYTNLTYQFFLDTSVVDFVFAHHQKLVGAIAQKDSKKAV
                     QIMSSLLDHGQRHLKTFIQKQQLKEAL"
     gene            5346..6059
                     /locus_tag="PLV81_13975"
     CDS             5346..6059
                     /locus_tag="PLV81_13975"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOM11246.1"
                     /translation="MFQFFDTDSDSAFGAISYFDAIKEQYDAVVPQAVYGSDTARIHI
                     SEKTIAIDFVQALHEPVTRYEFTLPHIHAFGNGKHPTTYMCLALLAETLHTRITKKPY
                     EITMLDAGTGTGILAIAAAVMGVTHIDAFDASIQAYECARNNIGNKFPITVQCCDIQS
                     FEPKRMYDCITANLMTALIIASAPKFSSMLENQGYCIVSGIIDERQYEVIETFRKYNC
                     ILKDGQTSQGWFAGVFQKL"
     gene            complement(6060..7217)
                     /locus_tag="PLV81_13980"
     CDS             complement(6060..7217)
                     /locus_tag="PLV81_13980"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOM11247.1"
                     /translation="MKADVYPAPDVYHFTYCLKVSKPNPPQVNITFDDVVAYQYTGGT
                     TGVSKGAILTHRNLGCMVQMYEQWFKTNRGEEIAMAAPPIFHVLGMSAAMNLPLYMGW
                     TIVLIPKPQPENLLKAIRLYRPTMSPLVPTMYVGMLQHPDLKKTDMTCFKLLTSGGAS
                     LPVEVLQEFKKLTGVEINEGFGMTETSPQTHLNPYKGKKKPGSIGIPYPDTEVKIVDL
                     ETGTKEVPVGKPGEMLFRGPQVTQGYHNKPEETKKAFTKDGFLKSGDIAYMDKDGYFF
                     VVDRKKDLIISGGYNIYPREIEEVLYQHEKVAKCAVIGIPDTKRGENVKAFVVLKEGQ
                     SATPDELLDFCKERLAKYKWPVEIEIRKSLPESNVGKILKKELRTEIAKTK"
BASE COUNT         1994 a         1697 c         1492 g         2316 t
ORIGIN      
        1 aattaataaa aaacattaac taacaaaaca tcaatacaag gacaatttaa aaatccttta
       61 ccggtgttag cggtacccgg ggatatatct gaccatggac tggtggcata gaattatata
      121 cccagttgat atcacacata ccaagcgtaa tacctgcata ccgcctgtag cctttgaaaa
      181 tcccccgtgt cagtatattt tccatcttta ctgttagagc atacactgca accggtgttc
      241 cttcttttaa ttgtaacaat tcttcacgaa aagctaacgg cgagaacact atggtagagg
      301 aatcactggc ctttgcctgc agtaccggca ctatttttgg aaaaccatca ctgtcaatcc
      361 aggcggcaaa tttaagcgca tccagcctgt taaaaagttc aaagcctggt gtatcaaggg
      421 gcctggtgtc ttttttctgt tttgcagccc attttgaaca tagcgttgca ataacagctt
      481 ttgcgatagc tgccaaaggc aagcgttcct tttcatttgc ctcaagtaaa tcaaaataat
      541 gcacagtatt aatgccaaag tatgtattgt atctgaacat gggaaattca ttgaacatct
      601 catattcagg accttctttg gcactgtgcg tataacgtgc tttcccacgc cacatatcca
      661 tattcatggt aagtatgcaa aaacctatgt tattgttgta ctttatatat tctttgctta
      721 caccttttga aaattcaccc agtgccatat gtgtgtcatc gagtgcaagt agtgacgtga
      781 gcaatgttac atggggtaat ccttcgttat taattgtggc aacaagtgct attttttcat
      841 ttggttttaa tgctgcaatt tcatcaatgg tgaatgattt tcgtgttttc atttatattc
      901 ctccatacat actattagca tcctaatttc ttctttggtt ttgttaccgg tgcttcatca
      961 tcttctatac gggttgaacc gcttgttttc acaattgccg gattattgca atgctgcttt
     1021 gccagtgctt caaaatggct tagttcctct ttagttaaaa gtggcgtatg tgccagttcc
     1081 cattccagcc ccagcctggt atacttgtcc ttgcatagat tattaaatgc gcacaaatcc
     1141 cagcgagtaa tagcttttgg attaatagat agcagattgt ttatttcacc agctatcgcc
     1201 acgatatttt catctcgtgc agttttaccc gggataagtg gcgtcctaat ccacacctca
     1261 cgtgggaaca gatgttcttt cataaaagaa agtatatatt tgatattatt aagcactcta
     1321 taatttgact taccggtaaa ctgttggtgt tttagaggat ccatttcttt aagatcatag
     1381 agcacaagat caacatgggg cagcagcata tcgagcgtat gttgattgca tataccacag
     1441 gtgtcaattg cggtgtgaat gccatgatgt ttgagttcca ccagcacctt ttttgcaaaa
     1501 tgagcctgga aggtactttc accacctgaa atggttacac cacctcctga ggtgccaaaa
     1561 tatgcattgt ccttcaaaag ctcctgtacc agtgcatcag cactccactg tttgcccaaa
     1621 agctccatgg cagttgaagg acacacttca gtacacctac cacatccttt acacacagtg
     1681 cgattaatat gtatgccatc atcaaacgtc agcgcgccat tgggacatgc ctgcacacac
     1741 gttttacatc caatgcagcg ggcaccaatc cactgcacct gcggttttgg atttatgctt
     1801 tcaggattgt ggcaccacac acacttgagc gggcagcctt tcataaatac cgtggtgcgt
     1861 atacccgggc catcctcagt agacatgcgc tgtatttcaa gtactatgcc agtggaatca
     1921 atagtcataa ctatctccta aaacttgttg tgtgaaacac gagcaataac ctcatcctgt
     1981 aattccttac caatggaaac aaagtacgca ttgtatccag ttaccctgac aagcaggtgc
     2041 cggtaatcct gaggatgttt ctgtgcatcg cgcagcatgt ctggatcaag catattaatc
     2101 tgcaatgcag taccgccatt tattgcatac ccacgtaaaa aggccttgaa ctttcccctg
     2161 tgttcaggat ctttcagtat tgaaggatta aacgtaatgg tatggcttgc accgttagga
     2221 agaaggttaa catatccgcc aaaatcaccg ggggtatccc tgccacccag tacctttcca
     2281 acggaatttg cgttggctgt aggtccattg atgtcagccc cgttaaccgg acatattgca
     2341 tttgataaaa actgaccctt ttttctgcca tcaggagttg ctggcagaat atagccatca
     2401 ccaacccagt aattccatga tagcatcccg ggcctgaatt gccgtcctgt ggatttggtt
     2461 ttatgtttcc atgtttcttc agtccacaat tgcataacct taaggcccat agcatcagct
     2521 tcatcatcat ctctgccata ttttggtgct cggttttttg caatgctctg taaatattca
     2581 tagccttccc agtttgcttt aagtgcctga ataagttcat ccatggtgca aacttttttg
     2641 tcatatacca tatacttaat ggcaagaagc gaatccactg ttgttgcata ggttactgct
     2701 tcaagcgtgg taaagcttat ttctgctgca ccctgtgtta tgtctttacc cttttccgca
     2761 caccctttca ccaggcatga cagatatggt gttggatggt actgtgcacg tatgcgttca
     2821 gattcttcgt acagttcaac aatctttttt atgataaact ttgtttgaat ctcatacgca
     2881 ttccagaact catcaaatgt gttaaatgta cgtggatcac ctgttctggg tccaatctgt
     2941 tttactggct ctactttacc ggtcaccgga tcagtaaatg ggaagaaatc cttaccgttg
     3001 tttaacgcaa gctcaattgc ctttgccagg ttaagattat tgtctaccgt gcctgagcgg
     3061 tcattgccaa ccatggtatt ttccagacac ccaacaggtg cataatcata cacgttattt
     3121 tcgtttatta aatgctcaat gccagccttt tttgcctcac gaatcatccc tgccattgaa
     3181 cgctcatcaa agttgagcag gaatggagca ccctgacagg atgcaatcat atcaacaact
     3241 ttatcaagca gcgcatcagg gcttttctgg tgtagcctta tattgggttt tggctcaagt
     3301 attggcgaca tctcatcaat gacttcaagg aatgcaaagg tcaggtcatt tgccatgtca
     3361 ttgccatcct ttcccaatcc ggcaagcgtt atgagttgcc catagccgga tgtaatgccg
     3421 ttattccccg tacgtatcat ggcatcgtat gcagtgttgg cgtgtataaa aaagcatttt
     3481 aatatctctt taccaaattc gcggtccatc ccctttgtca gcgagtactg ccagtacgga
     3541 tacagatact ggtcaatgcg accaaatgac acgcccgggc caggataatt ttcatcgctc
     3601 atcactagca tgtgagcaag ccacagtgac tgcacagctt cccagaaact gactgctggt
     3661 tcccacggca ctcgctccag gtttgcagcc atctgctcaa gttcttccct gcgtgcggag
     3721 tttgattctt cttttgcaag acgtttgcat tcttttgcat actgcagcgc aacatcacgc
     3781 ggcatggtag ctgctgtcat catagcacga agcacagccc ctttgttgcc ttttttttct
     3841 ttgggcgata gctcattgta aaagccttct atttcagcat aaataccctt aaaacctttc
     3901 tgtaacactt tttgataatc aggaatcagg tgaccactgg ttgcaccagc gttgccaaag
     3961 ccatggtcat ggaaccactt catttttgca cgtgcaccgt ttttccccat caccagtctg
     4021 atgcgctttt tcatttcact tttggtaagg caaaacgatg tctgtatatt aaaccgggca
     4081 ccagcaatca gatcaccagg caaaatttca tgcggcaggt agttaaccat tacttctttg
     4141 ttaaaccacg cacgccgctc agggatgctc catgaataaa agtctttggg tacatctact
     4201 ttatgtgctg ccatcctgaa tgactgatac atggtttgca tgagcgtata cgtttcaggt
     4261 acaatgtaaa atgtcatttc gttaaattgt gcatcccacg gcgttcctgt ggtaaaactt
     4321 ttatactcat tgttccattt cctttcagct cctttaaagt agtaatcacg aagccactga
     4381 atccgtggtg ataaattttt aggctgtttt aattgtgcct taggtgatgt ctggctcata
     4441 atgcctcctt taattgttgc ttttgtataa atgttttcag atgacgctgc ccatgatcaa
     4501 gcaatgacga cattatctgt actgcttttt tgctatcttt ttgggcaata gctcctacca
     4561 gtttttgatg atgtgcaaac acaaaatcaa caacactggt atcaaggaaa aactgatatg
     4621 tgagattggt atacacctga cggaatgaat ttaagagtag cgggtacacc acattgccgc
     4681 ttgccagtgc tatgaggtga tggaatttaa aatcaacatc cactattgcc tgcgtctgtg
     4741 caggtataag cgattgttcc tcagtaatta tcgccctgaa ttcatcaagc tgttgctgtg
     4801 ttctgtgttg cgctgcaagt tttactgttt caagctcaat aagcgtgcgg gtagcaagca
     4861 agctttctaa gatatgtggc tcaaactggc cagttgcata cgaaacaagt gattgcaaaa
     4921 tggcaagtga accctcacgg cgatagtcat tgacagttac accatgacgt ggccgtattg
     4981 tcacaagtcc tttttgctcc aggataataa gtccttcatg gacaactggg cggcttacac
     5041 caagttgttt tgccagttca cgctcggatg ggattttctg cccgatggtg agtttgcctg
     5101 atataatgag ttcctcaaag cgcttcacaa acgcttctac caggctatca gcttttaaag
     5161 gttctaagta cataatgcct cgttatggtt ggtggtatta ccaccaacca atgtaattac
     5221 gaccaagtat aatgtcaata ctttttacaa aattttttta tcgaataact atcgcacaaa
     5281 attcttgatg tcattgatgc cgcctggtat agtgtatcaa ttcatcgttg tagtgaggtt
     5341 acacaatgtt tcaatttttt gatacagata gtgatagtgc atttggagca atttcatact
     5401 ttgatgccat aaaggaacag tatgatgctg tagtccctca ggcagtctat ggcagtgata
     5461 cggcaaggat acatatttca gaaaaaacaa ttgccattga ttttgtacag gcgctacacg
     5521 agccggttac acgctatgaa ttcactttgc cgcatataca cgcatttggc aacggcaaac
     5581 atcctaccac ttatatgtgt ctggcattac ttgccgaaac attacacaca agaattacca
     5641 aaaagcctta tgaaattacc atgcttgatg ctggtacagg aacagggatt ctggcgatag
     5701 ctgcagcggt tatgggtgtt acacatattg atgcatttga tgcaagcatt caggcctatg
     5761 aatgtgcacg taacaatatt ggcaataaat ttcccattac tgtccagtgt tgcgatattc
     5821 aaagctttga accaaaacgc atgtacgatt gcattacagc aaacctcatg actgcactta
     5881 tcattgccag tgcccccaaa ttcagttcaa tgcttgaaaa ccagggatac tgtattgtca
     5941 gtggtataat tgatgaacgc caatatgaag taatagagac attcagaaaa tataactgta
     6001 ttttgaaaga tgggcaaacc agccagggct ggtttgccgg tgtgttccaa aaactataac
     6061 tatttcgtct ttgctatttc agtacgcaat tcctttttaa gaatcttgcc aacattggat
     6121 tcgggcaagc tctttctgat ttcaatttca acaggccatt tgtattttgc caggcgctct
     6181 ttacagaaat caagcaactc atcaggggtt gcgctctgac cctcttttaa aacaacaaac
     6241 gctttaacgt tctccccacg ttttgtatct ggtatgccta taacggcgca tttagcaact
     6301 ttttcatgct gatacagcac ttcttcaatt tcacgcgggt atatattgta tccacctgaa
     6361 atgatgaggt ctttcttgcg atcaacaaca aagaagtacc catctttatc catataagca
     6421 atatcaccag attttaaaaa tccatccttg gtaaaagcct ttttggtttc ttcaggttta
     6481 ttatgatacc cctgtgttac ctgaggaccg cggaaaagca tctcaccagg tttgcccacc
     6541 ggcacctctt ttgttcccgt ttcaaggtca acgattttaa cttcagtatc aggatacgga
     6601 atgccaatgc ttcctggttt tttctttccc ttatacggat taagatgcgt ttgtggcgag
     6661 gtttcggtca tgccaaagcc ttcattaatc tcaacaccgg taagtttttt aaactcctgc
     6721 aatacttcaa ccggcaatga agcgccaccc gatgttaaca gcttgaagca ggtcatatcg
     6781 gttttcttta aatcaggatg ctgcaacatg cctacataca tagtgggcac cagcggcgac
     6841 atggttgggc ggtacaggcg tatagcttta agcaagtttt ccggctgtgg ctttggtatg
     6901 agaactatgg tccatcccat gtacagtggc aaattcattg cagctgacat gcccagcaca
     6961 tgaaatatgg gtggcgcagc cattgcaatc tcttcgccac ggttggtttt aaaccactgt
     7021 tcatacatct gcaccataca tcccaggttg cgatgcgtta aaatagctcc ctttgatacc
     7081 cctgtagttc cgccagtata ctgataggct acgacatcat caaaggtaat gtttacctgc
     7141 ggaggatttg gctttgacac cttgaggcaa tatgtaaagt ggtacacatc aggtgcgggg
     7201 tacacatctg ctttcatatt atttttacgt gcaacaagag gaaaaagaat atttttaggg
     7261 aatgggagat agtcaccgga tactatgaac tatcgcccaa atgataaacc attacagtat
     7321 gcatttgtat tatattgttt cagaatccct gataaacgat gccaagtact ttttatgttc
     7381 ttctagtgca gcaatcagtt gttctagatg tgtaacctgc ccggcaactt cgctgacgta
     7441 tgtttgtttc ttctggatag cgacatcgat tgcttttcgg gtaatggtaa taacatgag
//
LOCUS       DAORYL010000111         7476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_7014_length_7476_cov_12.481741, whole genome shotgun sequence.
ACCESSION   DAORYL010000111 DAORYL010000000
VERSION     DAORYL010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7476
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7014_length_7476_cov_12.481741"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="PLV81_13985"
     CDS             <1..1092
                     /locus_tag="PLV81_13985"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOM11248.1"
                     /translation="MRHKPHLKGKEYLKHIPNLVIFTPTHDSYFEVPSLSKVYYALKP
                     RPHFVIMAKRDFLSGNYLSSNFGKKNKLAYYLLYFLDRTGLPKAFFKKMKLIDIPRPF
                     IEDFNNKEAMKSEIYNQMEKLKSSIHSGFSTLIFPEGTTWGFGGLKNLRSGIFQLVDS
                     TYRHFQKKLYILPINVKVDRCMIGKKDIFINIGKPVFILDEKDKFLAKVQEILKDMHT
                     ITFSQIASVYLQRLSMAVEEIDRVIVLKKEEITSHLDSIIADIMALVKEGRLPNIDEH
                     LNDRSYRHKKLKRFLKFCIKKGYIVRSNGTYIINTKKVLASFPIKSYRKENPLGFSAN
                     ELLSINYNVLIAVYNKYCNSFRSISAHAM"
     gene            complement(1097..1243)
                     /locus_tag="PLV81_13990"
     CDS             complement(1097..1243)
                     /locus_tag="PLV81_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11249.1"
                     /translation="MMGHEDILIVSVALFNTLDLQLLKNLLSTPKVQQNSKEGVRKGI
                     HAIQ"
     gene            complement(1240..2550)
                     /locus_tag="PLV81_13995"
     CDS             complement(1240..2550)
                     /locus_tag="PLV81_13995"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016551180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOM11250.1"
                     /translation="MPTTIKNLGPCTIDSPLLKKYSQRDAAFVGDDIRIRLNAFIGKN
                     PPKGGEHIDSFEAAGPREKIFFDPEKTRVAITSCGGLCPGINDVIRSLVMESYYGYGV
                     RNIIGIPYGYNGLNPDYNYKPIELTPDFVKSIHMQGGSILGSSRGGTKDMDRLVDTLE
                     RLNVNILYTIGGDGTLRGAHDIAMIAEKRGLKLAVVGIPKTIDNDISFIQRSFGFETA
                     FSIAVDAIYAAHTEAEGAPNGIGLVKLMGRHSGFIAANAALASSDVNFVLVPEVPFEL
                     EGPNGLCAHLEERLQKRGHAVICVAEGAGQEILQKNSEKKYDASGNVKLADIGVYLKE
                     KIQEYFDKKGIEINLKYIDPSYMIRSAPAVPNDSIYCTLLGQNAVHAGMAGKTDMVIG
                     QWSNIYTHVPIEVAISQRKRIDPRSRFYSSVIDATGQPSAMINE"
     gene            complement(2566..4002)
                     /locus_tag="PLV81_14000"
     CDS             complement(2566..4002)
                     /locus_tag="PLV81_14000"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HOM11251.1"
                     /translation="MSIQTLITLFDWRSLIDIPILSFIVFLFYTTLRSSGSWRIGLGI
                     IVALLFYLVARFIRLPGVEWVFNNVSNIALIALIVIFQPEIRKIFERAASAFKVKKLL
                     KESSGFINEIAKAAFILAERRWGAIIVIPGKDEVENKINGGFVINAEVSVPLIVSIFD
                     PHSPGHDGAMLVENGKIVGFGYRLPLSASEKLDYQFGTRHHASLGLSEVSDALIITVS
                     EERGTVSLFHSGSYVQVDSEKKLERELKSHLQNVSMLFPVSTKRRARSIRLAEAFLSL
                     IVGCCIWLAIAINASQVVQRTYTIPVQYNLPPKMVIIGEKPQTVRISVSGSASEIESI
                     KAYELKAFIDLTDVSPGEHLVNLQRKNFNVSNDVNIIDIEPPQLKLVLHTMEQRDVEI
                     KPQLVGALPSGYEITSIDLSPSKIPILYTTDLENPEDIYLTTAPIYINGIQQSVKLTT
                     KIVAPKGVYPLDASNWPDVTVNIYVHKK"
     gene            4217..5545
                     /locus_tag="PLV81_14005"
     CDS             4217..5545
                     /locus_tag="PLV81_14005"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HOM11252.1"
                     /translation="MPAQSNDIHFPKGFLWGTATAAHQIEGGNTNNNWWLFEKKGKIK
                     NNDSALIACDHWNRYKQDFDLLKKLNCNAYRMSIEWSRVFPQPGTLDKKALDHYVTMI
                     DELLKRKVTPFVTLFHFTLPIWWCNEGGLLNRDRKHLQHFSQFCRVLATTFKGKVKYY
                     NTINEPSIVMLGYLTDMFAPGEKNIAKAIKALGTVMLMHAIAYHTIKSVDPYAKVGIV
                     HNVQIPMPYNPKSIMDNLLAKFVDYLFNGAIIRALQSGKLPFQIFSRHNELVNSNDFI
                     GLNFYNFIRVSLKLKDLATSATDNPLCGTDKLCAGLGWEPYPEGFYLAIKRMHHAFPN
                     LPMYITENGIGTDNDAWRQNVLIDHLKMMHKALQEGIDIKGYFYWSLIDNFEWAQGYM
                     SRFGLVAVDFKTQKRTIKPSGKLYAQIAKANAISSSILKKFPQDIYRPIV"
     gene            complement(5625..6449)
                     /locus_tag="PLV81_14010"
     CDS             complement(5625..6449)
                     /locus_tag="PLV81_14010"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOM11253.1"
                     /translation="MSEKAFLVDISKCQGCRGCQVACKQWNGLPGEKTTFFGGPEYTN
                     PANLSALTWNHVVFYPLDKSNPERPVWNIKHTKCYHCAEANCIAVCPQMAISKVDGWT
                     VIDQEKCIGCGACVSACVYKVPGLSEINHKNDMGQLIIHRGKSHKCNACTLNKRDIPA
                     CVSTCPSGALQFGHRQAIIKKALKRVKELKKDFPNASVYGLEEFGGLGVITVLRDKPE
                     KYGLPVGKDAQKIDFSHYESINSIYHTLSVFTMGIPYLQKKAYTVAKALAGTEKKA"
     gene            complement(6466..>7476)
                     /locus_tag="PLV81_14015"
     CDS             complement(6466..>7476)
                     /locus_tag="PLV81_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HOM11254.1"
                     /translation="VANPDPILKAYWNFTPEGHHEPDPHMVAKEINGFEWKKNGEHGD
                     QIENFTKLTDDGKTVCGNWLYSGSYNEDGNNMARRSKKDKTRMGMYHGWAWAWPMNRR
                     IIYNRASVNMKGEPWNPKKPVIWWDGDEWKGDVPDGPAPAGEKLPFIMLPEGVGRLFA
                     PTLNDGPFPEHYEAVESVTRNIFNSQQYNPAVSLYPGTQGEFGTSARFPYIGTSYRVT
                     EHWQAGAMTRNLPWLDELVPDMFCEISPSLAAKKGIKNGDKVIISSQRGSIETYALVT
                     ERVQPLKVNGQMVEMVGMVWHFGYGGHASGSSCNFLTPQCGDPNTFIPEFKVFLVDIR
                     KA"
BASE COUNT         2186 a         1663 c         1335 g         2292 t
ORIGIN      
        1 atgcgacata aacctcatct taagggtaag gaatacttaa aacatatacc caatttagta
       61 atattcaccc caacccatga cagttacttt gaagtcccat cgctttcaaa ggtatattat
      121 gcattaaaac cgcgtccgca ctttgttatc atggccaaaa gggatttttt aagcggtaac
      181 tatctgtcat caaattttgg taaaaaaaac aaattagcct attatctatt gtattttctg
      241 gacaggacag gacttcccaa agcttttttt aaaaaaatga aactgattga tatcccgcgc
      301 ccgtttatag aagatttcaa taacaaagaa gcaatgaagt ctgaaatata caaccagatg
      361 gaaaaactta aaagttccat tcacagtggg ttttccacac tcattttccc tgaaggcact
      421 acatggggct ttggcggttt aaagaatctt cgaagtggta tttttcagct tgttgacagc
      481 acctacagac attttcaaaa gaaactctat attctcccca tcaacgtcaa agttgacaga
      541 tgtatgatag ggaaaaaaga tatttttatt aatattggca aaccagtttt cattcttgat
      601 gaaaaggaca aatttttagc taaggtacag gaaatattaa aggacatgca taccattacc
      661 ttcagccaga ttgcgtcagt gtatctgcag cgtttatcca tggctgttga ggaaattgac
      721 agggtaattg ttcttaaaaa agaagagatt acatcacatc ttgattctat aattgccgat
      781 attatggctc tggtgaaaga aggaaggctt cccaatattg atgaacatct taatgacagg
      841 agttatcgcc acaaaaaatt aaaacggttt ttaaaatttt gtattaaaaa aggatacatt
      901 gtcagaagca atggcacgta tataatcaat actaaaaaag tgctggcatc tttccccatt
      961 aaatcatacc gcaaggaaaa tccccttggt ttttcggcaa atgaattatt atcaattaat
     1021 tacaatgttt taattgcagt atacaataag tattgtaata gcttcaggtc tatttcagca
     1081 cacgccatgt aagctgctac tgaattgcat gtattccttt tctaacacct tctttactat
     1141 tttgctgaac ctttggtgtt gatagcaaat tttttaaaag ctgcagatca agggtattaa
     1201 acagtgcaac tgatacaatc agaatatctt catgtcccat cattcattaa tcattgcaga
     1261 aggctgccct gttgcatcaa ttacgctact gtaaaatcgt gaacgcgggt caatgcgctt
     1321 tcgctgtgat atagcaacct caataggaac atgggtatag atattactcc actggccaat
     1381 aaccatatcc gttttgcctg ccatgcctgc atgcactgca ttctgcccca gaagcgtgca
     1441 gtagattgaa tcattgggaa cagcaggtgc acttcgtatc atgtagcttg ggtcaatata
     1501 ttttagatta atttcaattc cctttttgtc aaaatattcc tgtatttttt ctttaagata
     1561 cactccgata tctgcaagtt taacattgcc tgatgcatca tatttttttt ctgaattttt
     1621 ttgtaaaatt tcctggcctg ctccttcggc cacacatata accgcatgac ctcttttttg
     1681 caaacgctcc tctaaatgtg cacacaaacc attcgggccc tccagttcaa aaggcacttc
     1741 aggtaccaac acaaaattaa catcactgct tgcaagtgcc gcatttgccg caataaatcc
     1801 agagtggcgc cccataagct ttaccaggcc aattccattt ggagcacctt ctgcttcggt
     1861 atgtgctgca taaatagcat ccactgcgat ggaaaatgct gtttcaaagc caaatgaacg
     1921 ctgaataaag cttatatcat tatcaatggt tttgggtata ccaacaaccg ccagcttcaa
     1981 ccctcttttt tcggcaatca ttgcaatatc atgcgcgcca cgcaaggtcc cgtcaccacc
     2041 tatggtatat aatatattga cattgagtct ttcaagggtg tcaaccagcc tgtccatatc
     2101 tttggttccc cctctggaag agcccaatat acttccaccc tgcatatgta ttgatttgac
     2161 aaaatcaggg gttaattcaa tgggcttata attgtaatcc ggattaagac cattgtatcc
     2221 atagggtata ccaataatat tccttacccc atacccataa tatgattcca tgaccaggga
     2281 acgtatcaca tcattaatac cagggcacaa ccccccgcaa ctggtaatag caactcttgt
     2341 tttttcagga tcaaagaaaa tcttttcgcg tggtcctgct gcctcaaaac tatctatatg
     2401 ttcccctcct ttaggcgggt ttttacctat aaacgcatta aggcggattc tgatatcgtc
     2461 gcctacaaat gctgcatcac gctggctgta tttttttaac aatggcgaat cgatagtaca
     2521 tggccctaaa ttttttattg ttgttggcat aaaaaactcc ccacactatt ttttatgaac
     2581 ataaatattc accgttacat ccggccagtt tgatgcatca aggggataca caccctttgg
     2641 tgctactatc tttgttgtaa gtttaacact ctgctgaata ccattaatat agattggtgc
     2701 tgtcgtcaaa taaatatcct ccgggttttc aagatctgtt gtatataata ttggtatttt
     2761 tgaagggctt agatctattg atgtaatttc ataacccgat ggcaatgcac caaccagctg
     2821 tggttttatt tcaacatcac gctgctccat tgtatgcagt acaagtttta gctgaggggg
     2881 ttctatatca ataatattga catcattgct aacattaaaa tttttccgct gtaaattcac
     2941 taagtgctcg ccaggtgata catcggtaag gtcaatgaaa gctttcagct cataagcctt
     3001 aatactttct atttcgctgg ctgagcctga tactgatata cgcaccgtct gtggcttttc
     3061 acctattatt accattttgg gtggaagatt gtattgcaca ggtattgtat aggtacgctg
     3121 cactacctga cttgcattaa tggcaatagc aagccatata cagcaaccaa ctatcaggct
     3181 taaaaaagcc tcagcaagac gaatgctcct tgctcttcgt tttgttgaaa cggggaaaag
     3241 catacttaca ttttgcagat gactttttaa ctcacgttcc agttttttct cactatcaac
     3301 ctggacatat gaaccagaat ggaataggga tactgtccca cgttcttctg atactgtaat
     3361 aatcagtgca tcggaaactt cagatagccc taatgatgca tggtgtcgtg taccaaactg
     3421 gtaatccagc ttttcagatg ccgatagtgg cagcctgtat ccaaaaccca caattttacc
     3481 attttctacc aacattgcac catcatggcc tggcgagtga ggatcaaata tacttacgat
     3541 aagcggcacg cttacctcag cattaatgac aaagccgcca ttaattttgt tttcgacctc
     3601 atctttaccc ggtataacga tgattgcacc ccatcgccgt tcagccagta taaatgcagc
     3661 ttttgctatc tcattaataa atccagatga ttctttaaga agttttttaa ccttgaacgc
     3721 tgatgctgct ctttcaaata tctttcgaat ttcaggttga aatattacga ttaatgcaat
     3781 taatgcaata ttacttacat tattgaaaac ccattcaacc ccgggtagtc gtataaaacg
     3841 agcaacaaga taaaataata atgcaacaat gatgcccaat ccaatccgcc atgacccgga
     3901 agaacgcaga gttgtataaa acaaaaagac aatgaatgac agaatgggaa tatcaattaa
     3961 actgcgccag tcaaataatg ttatcagtgt ctgaatactc atgatagttt tctttcttat
     4021 tatagaatgt attggatgca aatgtaattt tatgcaatta ctttttttac tctatgattc
     4081 attcacaaga attttgttac agtaactatc gcccatactc atttttttat tgatttttca
     4141 tactggcacg cttttactta aagtttatat gattatatcc gtatgatttc atagtatata
     4201 ccaaaggaga ctgcatatgc cagcacaatc aaacgacata catttcccca aaggcttttt
     4261 atggggtaca gcaactgctg cacatcagat agaaggtggc aacaccaata ataactggtg
     4321 gctttttgaa aaaaaaggaa aaataaaaaa taatgatagc gctctgattg catgcgatca
     4381 ctggaaccgt tacaaacagg actttgacct tttaaaaaaa ttaaactgca atgcctatcg
     4441 catgtccatt gaatggtcac gggtttttcc acaacctggc actctggata aaaaggcact
     4501 ggaccactat gtaacaatga ttgatgagct tttaaaacga aaagtgacgc catttgttac
     4561 cctttttcat tttacattac ccatatggtg gtgtaatgag ggtgggctcc ttaaccgcga
     4621 caggaaacat ttacaacatt tttcacagtt ctgccgcgtt ctggcaacaa cgtttaaagg
     4681 caaggtcaaa tactacaata ccatcaatga gcctagtatc gttatgttag gttaccttac
     4741 tgacatgttt gcccccggtg aaaaaaatat agctaaggcc atcaaagcgt tgggtaccgt
     4801 tatgctgatg catgctattg cctaccatac aatcaaatct gttgacccct atgcaaaggt
     4861 tggcattgtc cataacgttc agattcccat gccatataat cctaaaagta ttatggacaa
     4921 tcttttagca aaatttgttg attatctttt caacggagct attatacgag cattgcaaag
     4981 tggtaaactt ccctttcaaa ttttttcacg gcataacgaa cttgttaatt caaacgattt
     5041 tattggcctc aatttctata attttatcag agtatcactg aaactaaaag accttgcaac
     5101 atcagcaacg gataaccctc tgtgtggcac agataaatta tgcgccggcc ttggctggga
     5161 accctatccc gaaggctttt acctggcaat caaacgaatg catcatgcgt tccccaacct
     5221 tcccatgtac atcaccgaaa atggtattgg caccgataac gatgcctggc gacagaatgt
     5281 gttgattgac catctcaaaa tgatgcataa ggcattacag gaaggcatcg atattaaagg
     5341 ctacttctac tggtcactca ttgacaattt tgaatgggct caaggatata tgtcacgatt
     5401 tggtcttgtt gcggttgatt ttaaaacaca aaaacgaaca ataaaaccaa gtggtaagct
     5461 gtatgcccag atagcaaagg caaatgctat atcctcatca atactcaaaa aatttccaca
     5521 agacatttac cggcctatag tttaatatgt gcgatagtta ccgtaaaaaa gggcaggtgt
     5581 tgccacctgc cctttctgtt accttaacaa tatgtagtgt gtgcttatgc ttttttctcg
     5641 gtgcctgcca gtgcttttgc aacagtgtat gcttttttct gcagatatgg aatacccata
     5701 gtaaatacag acaatgtatg atagatgctg ttgattgatt catagtgtga aaagtcaatc
     5761 ttctgagcat ctttgccaac aggcaatcca tatttttcag gcttatccct gagaacagtt
     5821 atcacgccaa gtccaccaaa ctcttcaagc ccatacacac ttgcgttggg aaaatctttt
     5881 ttaagttctt taacccgttt cagggctttt ttgattatag cctggcgatg tccaaactgc
     5941 aatgcaccgg agggacaggt tgaaacgcat gcaggaatat cacgcttgtt cagcgtacag
     6001 gcattacact tatgcgattt tccccggtga atgataagct gtcccatatc attcttatgg
     6061 tttatttcac tcaagcctgg tactttatac acacatgcac tcacacatgc accgcatcca
     6121 atacattttt cctggtcaat aacggtccag ccatccacct tggatatagc catctgagga
     6181 catactgcta tacagttagc ttctgcacag tgatagcatt tagtatgttt gatgttccat
     6241 actggccttt ccggattgct tttatctaag ggataaaaca ccacatggtt ccaggttaat
     6301 gccgacagat tggcaggatt ggtatactcc ggacccccaa agaaggtggt tttttcaccg
     6361 ggcaatccat tccactgctt gcaggcaacc tgacagcctc tgcaaccctg acattttgat
     6421 atatcaacta aaaacgcttt ctctgacata gtcccacctc ctcacttaag cctttcgtat
     6481 atcaaccaga aatactttga attctggtat aaacgtattg ggatcaccgc actgtggtgt
     6541 caggaagtta caggatgaac ctgaagcgtg cccgccataa ccaaaatgcc ataccatacc
     6601 aaccatctca accatctgcc cgtttacctt caatggctgt accctttcag ttaccagcgc
     6661 ataggtttca attgaaccac gctgtgatga gatgataact ttatcaccat tcttgattcc
     6721 cttctttgca gctaaacttg ggcttatttc acagaacata tccggtacaa gctcatctaa
     6781 ccatggcagg tttcgtgtca ttgctcctgc ctgccagtgt tcggttaccc tgtatgatgt
     6841 tccaatatac gggaaccgtg cacttgtacc aaactcaccc tgagtacctg gatacagtga
     6901 cacagccggg ttatactgct gtgaattgaa tatattccgt gttactgact ctactgcttc
     6961 ataatgttcg gggaatggcc catcattgag agttggagca aagagtcgtc caacgccttc
     7021 tggtagcatg ataaatggca atttttcccc tgctggagca ggaccatccg gcacatcacc
     7081 tttccactca tcgccatccc accatatcac cggctttttg ggattccatg gctctccttt
     7141 catattgacg cttgcacgat tatagataat ccttctgttc ataggccatg cccatgccca
     7201 tccatgatac atgcccatcc tggttttatc cttcttgctg cgtctggcca tgttattgcc
     7261 atcttcatta tagcttccgc tataaagcca gttgccacat actgtcttgc catcatcggt
     7321 aagctttgta aagttttcta tctgatcacc gtgttctcca ttctttttcc actcaaatcc
     7381 attgatctcc tttgccacca tatgaggatc cggctcatga tgaccttcag gagtaaaatt
     7441 ccagtacgct ttcagaattg gatcagggtt ggcaac
//
LOCUS       DAORYL010000112         7321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_7175_length_7321_cov_11.586155, whole genome shotgun sequence.
ACCESSION   DAORYL010000112 DAORYL010000000
VERSION     DAORYL010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7321
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7175_length_7321_cov_11.586155"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="PLV81_14020"
     CDS             <1..749
                     /locus_tag="PLV81_14020"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOM11255.1"
                     /translation="LLPVFCVAIVIVYAMTGREIMEKSDALPEPRTAISKATMVIYKG
                     GDTLTRTIEMMAMKVGQNDKVLAVVKEEPGGDITKVLTHTNKTGEDMQWVKMSNGKVK
                     RISSGDRSGAFVNSHIFYEDLRSRNIDDYEYTLMGEETVEGFPCYKVEAKPKPGKSIY
                     DKAIFFIIKSGEFQYFAVRIDIFYEGYLYKRLINYNLKKVDGIITPYKAVMYRLDKKG
                     ANLGRTEVTITNLQYNNPAINENMFNVGKL"
     gene            complement(1048..2028)
                     /locus_tag="PLV81_14025"
     CDS             complement(1048..2028)
                     /locus_tag="PLV81_14025"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HOM11256.1"
                     /translation="MKRQVIQIISSILLLHFTSFVFAKEKNILSQNPGELTDTDIIVS
                     QVSNDTGNNTPDTMNQPNGNNWPTPYTLVAFIGIPIGTLVYGMATWNWGEEDGFHVRH
                     EGFFGQNTANGGADKVGHAFSHYLAFRILHNYYDWSENGKNTKWFYSITTAAALGLMI
                     EVGDAYTSEYGFSYEDLISDLSGITLGIILEYSPTLDSLIGYSWQYVPTSRFYKKQNE
                     KPINFTTDYNGAKFVLNFRLAGLQNLGLKLPKFLRYIQFDFGYFTRNYRSGNITDEGP
                     YRSLYYGVSLNFMEIVKDFFDNPETRASRILQQPFKYYHIPAGYHEDYKL"
     gene            complement(2256..2990)
                     /locus_tag="PLV81_14030"
     CDS             complement(2256..2990)
                     /locus_tag="PLV81_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOM11257.1"
                     /translation="MLKKVLYVLYQPYKWVVFLPFLVISTLFFGFLTIILVPLTNPKV
                     ASYICGALWSRLNGYMTPMLVKVHGRENINHRQSYVIVSNHQSHYDVFVLYGWLGIDF
                     KWVMKQELRKVPGLGIGCEKVGHIFIDRSNTEKALASLNDAKKKIVNGTSVIFFPEGT
                     RSKDGKVKEFKKGAFKMAFDLGLPILPVTIVGTSKILPAHTINLFPGVVHLYIHKPID
                     INKYSEDKIEELMDTTKSIMTSVLEK"
     gene            3201..4514
                     /locus_tag="PLV81_14035"
     CDS             3201..4514
                     /locus_tag="PLV81_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOM11258.1"
                     /translation="MKHTVALTQYNAKGSIKKAIELSRAFKNLKPTDKVFIKPNIVFW
                     AKVPFPKWGVITTSSVLLEVVELLQDYSVNNITIGEGIVTLKPNDRETANHAFEYLGY
                     YSLATRYGVKVINIFDRPFEKVAFDNDIDISLNTDALHSDFLINIPVLKTHAQTVVSL
                     GLKNLKGLLNIPSRKKFHNPDENKNLHYMISRLYTLLPKGATIIDGIFTLERGPSFDG
                     KPRKSNIIIASEDTLSADLVGTRVLGHDPATIAYLDMASKNKGRKPDLSDVAVVGESI
                     DALASFHEYTFPYNKEGTLPEKLQKMGVQGLSYPKYDSTMCTYCAALNGAVLTAITFA
                     WQGKPWNDVEVLTGKKMQPSLEKKYTILLGQCMVNLHKNNTSINAIPVKGCPPDPKET
                     VKALHQAGIEVNPLIFEHLDIAPTYYMERYKNKPEFDEKFYTIDE"
     gene            4605..5243
                     /locus_tag="PLV81_14040"
     CDS             4605..5243
                     /locus_tag="PLV81_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11259.1"
                     /translation="MHNVRDNPFVVVFQEWYEKVISGNVSHEVILCIKDLLHIDEEEI
                     IHYMTNELGNTLTLYTSKLKDEQIQTITLLIDTPDDEYFIPSIILASAYASFEIKSTF
                     SSPHTPLLPKMDNMYHQLAFGKNIFFQFGGCDLDMISDVLFDIISLPDIAKSGILQQM
                     QTLVIIKILELMYNSCLLLMKGDRFAQLPKTKPFAFFAHLNDKDYIRLVTFT"
     gene            5289..6371
                     /locus_tag="PLV81_14045"
     CDS             5289..6371
                     /locus_tag="PLV81_14045"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOM11260.1"
                     /translation="MNVSFNIIIFGAIFTLLHGVEQIAGRARRTKIDYLTAIVMADVA
                     LILYNHAIMATDGLQKQLSLIFYLTSIYAIGPLNYLYYYSLLHNTLTISIHLLSHLLP
                     ASVIFCLELIYYLSPTHVKENIISSIYSQSINVFSILLFLGGILFVLYQLYFIYQCSK
                     VFNDVKIRKGLYLTLILESINITTPIPVLLWLITKKNHYYAIAGYMTIAVIVILFLTN
                     RRFPLLFHRIADAIRNKKYERNYLSNINKEYLHHQLLNIMKNEKLYLDPEINLESLSY
                     KLNVTPHQLSQFLNEYCHMRFNHFVNKYRIEEAKNILSMHPDANIISVAYHVGFNSKS
                     TFNKIFKQYTGKTPSSFKEGCKNSHH"
     gene            6498..>7321
                     /locus_tag="PLV81_14050"
     CDS             6498..>7321
                     /locus_tag="PLV81_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11261.1"
                     /translation="MDTLSQATIAEFEQLTANFCKKSLLHYIDEHHPDGNFTLLPSLV
                     DEACAIGIIAKANASAPGYDYGVWGKQTIDSNDAIQSSLIMLATLAKTCASFAFLVHI
                     QGIAANILIRNNMETDYHYPLLILYDGLFPPYYSLLCNPSRIPINYTMENNYLIHTSM
                     GYGYNPQYAIVCIPESTGWQSIYFNNTDTILLQTIKTHGIRGLTYRARFNSKITHRMS
                     ADTHTIEMLLAYFWLGLAAIATGVAYSAYQKAIEYTGERYQRGGLIKNIESVQMLLG"
BASE COUNT         2534 a         1288 c         1161 g         2338 t
ORIGIN      
        1 gtttgttacc tgtgttttgt gtggcgatag ttattgtgta tgcaatgaca ggcagggaaa
       61 taatggaaaa gtcagatgct ctgcctgaac ccagaactgc aataagtaaa gcaacaatgg
      121 taatttacaa aggtggtgat accttaacac gcaccatcga gatgatggct atgaaggttg
      181 ggcagaatga caaagtactt gcagtagtta aagaagagcc cggtggcgat attaccaagg
      241 tattgacaca taccaataaa acaggcgaag atatgcaatg ggttaagatg tctaacggaa
      301 aagtaaagcg catatcatcc ggtgaccgca gtggtgcatt tgtcaattca catatcttct
      361 atgaagattt gcgatcacga aatattgatg actacgaata tacactgatg ggagaagaaa
      421 ctgttgaagg ttttccctgt tataaggtag aagcaaaacc aaaacctggc aaaagtatct
      481 acgataaggc aatatttttc attatcaaat caggtgaatt tcagtatttt gctgtacgta
      541 tagatatttt ctatgaagga tatctataca agcggttgat taattataat ctgaaaaagg
      601 ttgatggtat cattacaccg tataaagctg ttatgtatcg actggacaaa aaaggggcta
      661 acttaggcag aaccgaagta accatcacaa atctgcaata caataatcca gcaattaatg
      721 agaatatgtt taatgtgggg aaattgtgat gtaataatgc actacttttt tattcagcgt
      781 ttttttgcgc gagcacgtat gtatgtttga tagcagagag ctcaaagcgc aaagataatc
      841 ataatcatag cttaattgtt acagggactg tcccaaaaga ggtcgttgag taaattttac
      901 aaacattata tcgtaatact cgctcattac gatacaattt tgccacaaaa tctggcaaaa
      961 ttactcaatg aacgttacta aatttgtatc gaaacgactt ttgggacatc ccctgatatt
     1021 tacatagtac acaccacctt gctgcaatta taacttataa tcttcatgat agcctgcggg
     1081 tatatgatag tatttgaaag gttgctgtaa aattctacta gcgcgtgttt caggattatc
     1141 aaagaagtct tttactattt ccataaagtt caatgagaca ccataataca ggcttctata
     1201 tggcccctca tcagttatat tcccgctgcg ataatttcgc gtaaaataac caaaatcaaa
     1261 ctgaatatac cgtaaaaatt ttggcagttt caatcctaaa ttttgaagac ctgccaatct
     1321 gaaattgagt acaaattttg ctccattata atcagtagta aagttaatgg gtttttcatt
     1381 ttgttttttg taaaaacgag aagtaggtac atattgccag ctataaccaa tgagtgaatc
     1441 taatgttggt gaatattcca gtattatccc cagggtaatc cctgacaggt cagagattaa
     1501 atcttcataa gaaaagccat attcactggt gtatgcatca cctacttcta tcataaggcc
     1561 caatgcagct gctgttgtaa ttgaataaaa ccacttcgta tttttaccat tttctgacca
     1621 atcataataa ttatgcaaaa tcctgaatgc cagataatgt gaaaaagcat gaccaacttt
     1681 gtcagcacca ccatttgctg tattctggcc aaaaaaacct tcgtgtcgaa catggaaacc
     1741 gtcttcttca ccccaattcc aagtagccat cccatatact aacgtaccaa tcggaatgcc
     1801 aataaaagca accagtgtat atggtgttgg ccagttattc ccatttggtt gattcatggt
     1861 atctggtgta ttattacccg tatcattact aacctgactt acaataatat cggtgtccgt
     1921 aagttctcct ggattctggg atagtatgtt cttttctttt gcaaatacaa aactggtaaa
     1981 atgtaataaa agtatagaag aaattatctg tattacctgt cttttcattt tcaaccccta
     2041 aaaatatttt acaatgaaac tattcaataa aaattatacg aaacaatata aattgtatct
     2101 caaattataa agaaaaatat aaaaaaagta ttacgtgatt atttagtaat ttttttcaaa
     2161 tatgaaaaga ataataaaat ataatgaaat aagccattta caatgtagca ttttatataa
     2221 tttggtgagc ataaataaag atcagcgtat agcaattatt tttctaaaac agaagtcatg
     2281 atggatttag tagtatccat caattcttca attttatcct cagaatattt attaatatct
     2341 ataggtttat gaatatacag gtggactact ccaggaaata aattaattgt atgcgccggt
     2401 aaaatttttg atgttccaac aatagttacc ggcaatatcg gaagccccaa atcaaatgcc
     2461 attttaaaag cacccttttt aaattcttta acttttccgt ctttactgcg ggtaccttcc
     2521 ggaaaaaata ttaccgaagt tccattaaca attttcttct ttgcatcatt cagtgatgcc
     2581 aatgcttttt ccgtgtttga tctatcaata aagatatgtc caactttttc acaaccaata
     2641 ccaagaccag ggactttcct gagttcctgt ttcataaccc atttaaaatc tatacccaac
     2701 cacccatata atacaaatac atcataatgg ctttgatgat ttgatacaat aacgtatgac
     2761 tgtctgtgat taatattttc tcttccatgt actttaacca gcataggagt catatatcca
     2821 ttgagtcttg accatagagc accacatata taactggcta ctttagggtt tgtcaaggga
     2881 acaagaataa tggttaaaaa gccaaaaaac aacgttgata taaccagaaa gggtaaaaat
     2941 acaacccatt tatatggctg atacaacaca taaagaactt tttttaacat aattatcacc
     3001 ttcaaaaaaa tatgataaac acattttgat ttatataaat ttttttgcaa caaataaaca
     3061 taatattact tatcccatta ttgttttgta acaatattta tttatacttg acaactttaa
     3121 atatgttcga tagttaccac aataacaggg ttggggcata actaatatat aaattttcta
     3181 taatatacat gaggtttacc atgaaacata ctgttgctct aacccaatac aatgctaagg
     3241 gaagtattaa aaaagctata gaactttcca gagcattcaa gaatttaaag ccaactgata
     3301 aagtttttat taaaccaaac attgttttct gggcaaaggt tcctttccct aaatggggcg
     3361 ttattacaac atcttctgta ttgcttgaag ttgtagaact gttgcaggat tacagtgtta
     3421 acaatataac cataggtgaa ggcatagtaa cacttaaacc taatgatagg gaaacagcta
     3481 accacgcatt tgaatattta ggatattatt cattagccac tcgttacggt gtaaaagtta
     3541 taaatatatt cgatagacct tttgagaaag ttgcatttga taatgatatt gatataagcc
     3601 tcaatacaga tgcattgcat agtgactttc ttataaacat tcccgtttta aaaactcatg
     3661 ctcaaactgt tgtaagcctt ggacttaaaa atttaaaagg attattgaat atcccttcac
     3721 gtaaaaaatt tcacaatcct gatgaaaata aaaatcttca ttacatgatt tccagattat
     3781 acactctact acccaaggga gctaccatta tcgacggcat atttacattg gagcgtggtc
     3841 caagttttga cggtaaacca cgtaaaagca atatcatcat tgcttcagaa gatacccttt
     3901 cagctgattt agtaggtacc agagtactgg gtcatgatcc agctaccatt gcttatcttg
     3961 atatggcatc caaaaacaaa ggacgaaaac cagatctttc agatgttgct gtggttggtg
     4021 aatcaattga tgctcttgct tcattccatg aatatacttt tccttacaac aaagagggaa
     4081 cattaccaga aaaattacaa aaaatgggtg tacagggact gtcctatcca aaatacgaca
     4141 gtaccatgtg cacctactgt gcagcactca acggtgcggt actaacagcc ataacctttg
     4201 catggcaggg aaaaccatgg aatgatgttg aagtactcac aggcaaaaag atgcaaccct
     4261 cattagaaaa aaaatatacc atcttgcttg gccaatgtat ggtaaatttg cataaaaata
     4321 ataccagcat aaacgcaatt cctgttaaag gttgtccccc tgatccaaaa gaaaccgtaa
     4381 aggctttaca tcaggctggt attgaggtta atccacttat ttttgagcat ttagatattg
     4441 caccaacata ttatatggaa cgttacaaaa ataagcctga atttgatgaa aaattttata
     4501 caattgatga atagtattga ttttaagaaa ttctataaga tttttacaag aatactatag
     4561 tatcttaaca gcatggtata ctatgattaa caggaggtaa aacggtgcac aatgtcagag
     4621 acaatccttt tgtagtagtg tttcaagagt ggtatgaaaa agtaatatca ggaaatgtaa
     4681 gccatgaagt tattttgtgt ataaaagatc ttttacatat agatgaagaa gaaattatac
     4741 actatatgac aaatgaattg gggaatacac taacacttta tacttcaaaa cttaaagatg
     4801 aacaaataca gacaataaca cttttaattg atactcccga tgatgaatat tttattccat
     4861 caataatatt agcttctgct tatgcaagct ttgaaataaa aagcacattt tcttcaccac
     4921 atactccact tcttcctaaa atggataata tgtatcatca attggcattt ggtaaaaata
     4981 tttttttcca gtttggtgga tgtgatcttg acatgatctc agatgtttta tttgacatta
     5041 tatctttacc ggatattgct aaatcaggca tattgcaaca gatgcaaaca ctggtaataa
     5101 taaaaatatt agaacttatg tataattctt gtttactatt aatgaagggt gatagatttg
     5161 cgcaattacc taaaaccaaa ccttttgcat tttttgcaca tctcaatgat aaggattata
     5221 tacgtcttgt tacttttaca taataaaatt aattactatt cttttgattc tgggcttgtt
     5281 tgctcattat gaatgtatct tttaacatca tcatatttgg agcaattttc accttactcc
     5341 atggtgttga acaaattgct ggaagggctc gtcgtacaaa aattgattac cttaccgcca
     5401 ttgttatggc tgatgtggct cttattctgt ataaccatgc tattatggca acagatggcc
     5461 ttcaaaaaca actatcgctt atattttatt taacatctat ctatgcaata ggaccactaa
     5521 attatttata ctactattcc ttattacata ataccctgac aatatctatt catttactaa
     5581 gtcatctatt gccagcaagt gttatatttt gtttagaatt gatttattat ctttcaccta
     5641 cacatgtaaa agaaaatatt atttcttcta tttattccca gagtattaat gttttcagta
     5701 ttcttctttt tttaggtggt atattgtttg tactttatca gctttacttt atctatcaat
     5761 gcagtaaagt ttttaatgat gtcaaaatac gtaaaggctt atatttaaca ttaattcttg
     5821 aatcaattaa cattacaact cctatacccg tactgctatg gcttattaca aaaaagaacc
     5881 attactatgc aattgcagga tatatgacca tagctgtcat agtaattctt tttctgacta
     5941 acagacgatt cccattgtta ttccacagaa tagccgacgc tatacgaaat aagaaatatg
     6001 aaagaaatta tttatcaaat attaacaaag aatatctaca tcaccagctt ttaaatataa
     6061 tgaagaatga aaagctatat ttagacccgg agattaatct tgaaagtctg tcttataaac
     6121 tcaatgtaac cccccatcaa ttatcacaat ttttaaatga atactgccat atgcggttca
     6181 atcattttgt aaacaaatat aggattgaag aagccaaaaa tatactttca atgcatccag
     6241 atgcaaacat catatcagtt gcatatcatg ttggtttcaa ttcaaaatca acatttaaca
     6301 aaatatttaa acaatataca ggcaaaactc cttcttcttt taaagagggt tgtaaaaatt
     6361 cgcaccatta attgtaataa agtaatattg tcttaccatg tccactgtta taacacaagt
     6421 cgacaacaat agcataatat gatagtatca agaccaataa tattatatta tactagtagt
     6481 ttggaggaaa gtataccatg gatacgcttt cgcaggccac tattgctgaa tttgaacaat
     6541 taactgcaaa cttctgtaaa aagagtttat tacactacat agacgaacac catcccgatg
     6601 gcaattttac gctattacca tccctggtag atgaagcgtg tgctatcggt attatcgcaa
     6661 aagcaaacgc aagcgcacca ggttatgact atggtgtatg gggtaagcaa acaatcgatt
     6721 caaacgatgc tatacaaagc tcccttataa tgcttgcaac attggctaaa acatgcgctt
     6781 cttttgcatt tctggtacac atccagggaa tagcagctaa tattttaatt cgcaataaca
     6841 tggaaaccga ttatcattat ccgttgttaa tactctatga tggattgttc ccaccatatt
     6901 attctttatt gtgtaaccca tcacggatac caatcaacta cacaatggaa aataactatc
     6961 tcattcatac cagtatggga tatgggtaca atcctcagta tgcaatagtg tgcatacctg
     7021 aatcaacggg atggcaatct atctacttta acaataccga tactatactt cttcaaacta
     7081 ttaaaacaca tggtatcaga ggattaacct accgtgcccg ctttaactca aaaattacac
     7141 acaggatgtc tgctgatacc catacaattg aaatgttact tgcatatttc tggcttggat
     7201 tagcagccat cgcaacaggc gtagcataca gtgcatatca aaaggcaatt gaatatactg
     7261 gtgagcgcta ccagcgtgga ggtttgataa aaaacattga atcggtgcag atgcttcttg
     7321 g
//
LOCUS       DAORYL010000113         7316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_7187_length_7316_cov_13.514117, whole genome shotgun sequence.
ACCESSION   DAORYL010000113 DAORYL010000000
VERSION     DAORYL010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7187_length_7316_cov_13.514117"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PLV81_14055"
     CDS             <1..393
                     /locus_tag="PLV81_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11262.1"
                     /translation="DGAPISRIVQYIKARFAEVYNRITGRTGPFWNERYKDSIIQFAK
                     DGFHYLLWLLWYLAYNPIRGRLCSDPTRYHFSSIRAYLDEDADVGVPIDHHDYFVQLG
                     KTFAERVAKFMRYEEYYRKKYSMILEWV"
     gene            550..1305
                     /locus_tag="PLV81_14060"
     CDS             550..1305
                     /locus_tag="PLV81_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOM11263.1"
                     /translation="MELEQYHIGCSGWNYQHWRGIFYPEEIPKSKWLQHYATVFNSVE
                     INATFYRKFKDSAYVNWYNKVPQSFCFSVKLSKYVTHIKRLVDVTDEVNSCIASASLL
                     KQKLGVILIQLPPSLKFDTSLVADFLSILPGNYRYTIEARHNSWICDSSFSLLQHYNV
                     AWCISDTAGRYPYYEEVTADFVYIRLHGSTQLYASNYSDNELHNWAEKILKWNTLTYC
                     YFDNDFNAHAVYNALSLKKLLMLASPSTCSDYM"
     gene            1387..2226
                     /locus_tag="PLV81_14065"
     CDS             1387..2226
                     /locus_tag="PLV81_14065"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOM11264.1"
                     /translation="MELIDLHCHTTASDGIYSPTGIIDYAIVHNVSVLAITDHDSIDG
                     LEEALDYAKQKLFELIPGIEFSIDFPHGSFHLVGMGIDYTNGDLRYELEHLQMLRKER
                     IYKIIKDLQLHGIEVTDEEVEKEALGNSPGRPHVARVLVKKGYAPTVSDVFTQFMVKG
                     KPGFVKKEKINFHRALQLIKASGGTAIVAHPVSLGFSTWDECEQLIKDFVDAGVAGIE
                     VYSAMHSDEDVTHLLHIAQKYSLLISGGSDFHGDKNEAMGYYGEGKPIPAGLAKQFLK
                     TVK"
     gene            2230..3042
                     /locus_tag="PLV81_14070"
     CDS             2230..3042
                     /locus_tag="PLV81_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015902858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOM11265.1"
                     /translation="MKVDRGYQKSFLIDIDGVIVRGSSLISGAKDFIDKLKKGNYPFL
                     FLTNNSYYTPKELSERFFALGLDVDEDRFYTSAMATASFLVYQKPGCSAYVIGGKGLI
                     QELERNGIVITYKNPDYVIIGETEEYDYARIIEATYLIQEGARFIATNPDLTGPTQRG
                     PVPACGALVAPIERVTGVRPYFLGKPNPAMMYWARKKLNVHSANCFMIGDRMDTDILG
                     GSEAGMTCILVLSGVTTLAMVDRFPYQPDYIFEHVGEIDPDAILSRRERVKE"
     gene            3375..4487
                     /locus_tag="PLV81_14075"
     CDS             3375..4487
                     /locus_tag="PLV81_14075"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOM11266.1"
                     /translation="MGKFSFNEEALKNLKITDTSILERLVDFEDARKFHLNKDRSRKD
                     KRMSLKEAVAEYVHDGDIMTDGGFCYVRTSIQSYFEVIRQQKKNLQFCSSPNTNHSYM
                     INAGCCRYSHNSYLGAEMRGIDRNYARQLKAGKVTILSEWSHGTMAQGFKAAQLGLPG
                     VFSKQLLGSDMLKYNPYVKVMQNPIRSDADPVVFIPAIYPDVSIIHVHAADKKGNARF
                     FGPAINDIAMAAATRKLIITAEEIVPESDIRYNNKGVYIPAMYVDAVVELPFGAVPGQ
                     MPGCYYWSRQWWEMAFRYAMKSDENFQHFLEYWVYSVKDQYEFIEKLGGAKWIAESRR
                     LTKAEEYDNEDIGVNFDYEEYTSGEWPEDMSKMRFY"
     gene            4525..5319
                     /locus_tag="PLV81_14080"
     CDS             4525..5319
                     /locus_tag="PLV81_14080"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOM11267.1"
                     /translation="MGNRSDKPMNPLEMLAYILSKQIKDHEVVYIGTGLPMIAGILAR
                     KTHAPNIVLVYESGAQDPMEGALPWSVGGPATWRKSPLIMEMAYSFGQLYNGYVDKAF
                     LGFAQIDMYGNVNTHMIGKDFLKPDHRLTGSGGNNDLSSLAENMILVGLQSPQKFVPK
                     VDFITSPGFLDGGDSRKKAGLLGNGPVCVISQAAVFDFEPETKRMRVKSLHPGVSFDL
                     CQLACGFELLKPEGEIPVTEVPSKEILEILRNEVDPRGVFTSIPGM"
     gene            complement(5607..7055)
                     /locus_tag="PLV81_14085"
                     /pseudo
     CDS             complement(5607..7055)
                     /locus_tag="PLV81_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2290 a         1203 c         1527 g         2296 t
ORIGIN      
        1 gatggtgctc cgatatcccg tattgtgcag tatattaagg cacggtttgc tgaagtgtat
       61 aatagaataa ccggcagaac gggaccattc tggaatgagc ggtataagga tagcatcata
      121 cagtttgcaa aggatgggtt tcactatttg ttgtggttat tgtggtattt agcctataat
      181 cccatacggg gaagattatg ctctgaccct accagatacc acttcagcag catacgagca
      241 tatttagatg aagatgccga tgtaggagta cctatagatc atcatgatta ctttgtgcag
      301 ttggggaaaa cgtttgctga gagggtagca aagtttatgc ggtatgagga gtattaccga
      361 aagaagtact ccatgatact tgagtgggtg taaaaaaagg ggtcagagcc tatatgcaaa
      421 aatacttgaa taaatacatc aagcatagta ttttgcaata tatacataag attcagcatc
      481 atccttgcta ttgatgtaat taaatagtga tatcttaaag cattgtgtaa ttgtataata
      541 aattaataca tggaactgga acaatatcat ataggctgtt cagggtggaa ttatcagcac
      601 tggcgcggaa ttttttatcc agaagaaatc ccaaaatcaa agtggcttca gcattatgct
      661 acagtcttta atagtgtgga gattaatgca acattctaca ggaaatttaa agattctgcg
      721 tatgtaaact ggtataataa ggttccacag agcttttgct tcagtgtgaa attgtcaaaa
      781 tatgtaacgc atatcaaacg gcttgttgat gttaccgatg aagtaaactc gtgtattgct
      841 tctgcatcat tattaaaaca aaagttaggt gttatcctta ttcaattgcc accatcactt
      901 aagtttgata catcgctggt tgctgatttt ctatcaatac tgcccggtaa ctatcgctac
      961 acaattgaag cacggcataa ttcatggata tgcgatagct cattttcatt attgcagcac
     1021 tataatgtgg catggtgtat atcagatact gcgggaagat atccatatta tgaagaagtc
     1081 actgctgatt ttgtgtatat acgattgcat ggttcaacac aattgtatgc atcaaactat
     1141 tctgataatg agttgcacaa ttgggcagaa aagatattaa agtggaatac attaacatat
     1201 tgctattttg ataatgattt caatgcacat gcagtttata atgctctatc gttaaaaaaa
     1261 ttattaatgc ttgccagtcc ttcaacgtgt agtgattata tgtaaaaaat gttttaaaaa
     1321 ttatcaggtt gtattttttc agggattgtg ctattataat aaaattctta agcaggttta
     1381 attgctatgg agctaattga tttacattgt cacacaacag catccgatgg aatctattcg
     1441 ccaacaggaa tcattgatta tgccattgtg cataatgtca gcgttcttgc cattactgac
     1501 cacgattcta ttgatgggtt agaagaagct cttgattatg caaaacaaaa gttatttgag
     1561 cttatacctg gcatagagtt cagtatagat tttcctcatg gttcatttca tctggtcggt
     1621 atgggcattg attataccaa tggcgattta cggtatgaat tagagcatct gcaaatgttg
     1681 cggaaagaaa ggatatacaa aattatcaaa gatttgcagc tacatggtat tgaagttact
     1741 gatgaagagg ttgaaaaaga agctctgggt aattcccctg gaaggccaca tgttgcacgt
     1801 gtgctggtga aaaaaggata tgcaccaaca gtgagtgatg tttttactca gtttatggta
     1861 aaaggaaaac ccggatttgt taagaaagaa aaaataaatt ttcaccgcgc tttacagctg
     1921 attaaagcaa gtggtggaac tgcaattgtt gcacatcccg tatctcttgg tttttctaca
     1981 tgggatgaat gtgaacagtt aatcaaggat tttgttgatg ccggtgtggc agggattgaa
     2041 gtgtattcgg caatgcatag tgatgaagat gtaacacatc ttttacatat tgcacaaaaa
     2101 tattctttgc ttatttcggg tggaagcgat ttccatggag ataaaaatga agccatggga
     2161 tactatggtg aaggtaagcc tataccagct ggtttagcaa agcaattttt aaaaacggta
     2221 aaatagatta tgaaagttga taggggatat caaaaatcat ttttaattga tatcgatggt
     2281 gtgatagtaa gaggaagttc acttatttct ggcgcaaaag attttattga taagctcaag
     2341 aaaggtaatt accccttttt atttttaaca aataattcgt attatacgcc taaagagctt
     2401 tctgaaaggt tttttgcttt aggactggat gttgatgagg atagatttta cacttcagct
     2461 atggctacgg caagttttct tgtgtaccaa aagcctggtt gttctgcata cgtcattgga
     2521 gggaaggggt tgattcagga actggaaagg aatggcattg ttataacata taaaaaccct
     2581 gattatgtaa tcattggtga aactgaagaa tatgattatg ctcgcattat agaagccaca
     2641 tatcttatac aggaaggagc acgttttatt gctaccaatc ccgatttaac tggcccaaca
     2701 cagcgtggtc cagtacctgc gtgtggtgcg ctggtagcac caattgaacg agttactggt
     2761 gtaaggcctt attttttggg taaacccaat cctgcgatga tgtactgggc acgtaaaaag
     2821 ttgaatgttc actcagcaaa ctgttttatg attggcgata gaatggatac tgatatttta
     2881 ggtgggtctg aagctgggat gacatgtatc cttgttcttt caggagtaac tacattagca
     2941 atggttgaca gatttccata tcagccagat tatatatttg aacacgttgg tgaaattgac
     3001 cccgatgcga tactctcccg ccgtgaaaga gtcaaagagt agaaatagaa tcatgtacat
     3061 aacattcaac atgtatgctg tatatgctta acgtaatata ctaaactata gactaacata
     3121 aaacaacaca ttatttaaat gttatacaaa attttatgac atacatatta caattatatg
     3181 ctaatatttt atacacaaaa ctattgacaa atttatatat aaaataataa gtacaaatta
     3241 atatattata aattatattt aataccaaac ggttggtaat tgaatataat ttttgtaata
     3301 tataaaattg tatgttataa ttattaatga ttttgaagtt tttttgaaat acttaataac
     3361 gcaggaggat gtaaatggga aaattcagct ttaatgaaga agcacttaaa aatctcaaaa
     3421 tcacagatac ctcaattctt gagcgattgg tagattttga ggatgcgcga aagtttcatt
     3481 taaataagga cagatcgcgc aaagataaaa gaatgtcttt aaaggaagcg gttgctgaat
     3541 acgtccatga tggtgatatc atgaccgatg gcggcttttg ttatgttcgc acttctattc
     3601 aatcatattt tgaagttata cgacagcaga agaaaaacct gcagttttgt agttcaccaa
     3661 ataccaatca ctcatatatg attaatgcag gatgctgccg ttattcgcac aattcttacc
     3721 tgggtgctga gatgcgaggt attgaccgca actatgcccg tcaattaaaa gctgggaagg
     3781 tcactatact ttcagagtgg agccatggaa caatggcaca gggttttaaa gctgcccagt
     3841 taggtttgcc gggggtattc agcaagcaac tgttaggttc tgatatgctg aaatataatc
     3901 cgtatgttaa agttatgcag aatccaataa gaagtgatgc ggaccctgtg gtatttattc
     3961 cggcaattta tccggatgtt tcaattattc atgttcatgc tgcagataaa aaaggaaatg
     4021 ctcgcttctt tggaccagca ataaatgata ttgctatggc agctgcaacc agaaaactga
     4081 taattacggc agaagagata gtccctgaaa gtgatattcg atataataac aaaggtgtat
     4141 atataccagc tatgtatgtt gatgcagttg ttgagttgcc ttttggtgcg gttccaggtc
     4201 agatgcctgg ttgctattac tggtcacggc agtggtggga aatggctttc agatatgcaa
     4261 tgaagtcaga tgaaaacttc cagcacttcc ttgaatactg ggtatatagt gtaaaggatc
     4321 agtatgaatt catagaaaag ttgggtggtg caaaatggat tgctgaatca cgccgattaa
     4381 ccaaagcaga agagtatgat aatgaggata ttggtgttaa tttcgattac gaagaataca
     4441 ccagtggtga atggccagaa gatatgagca agatgagatt ctattaaaat ttatttttaa
     4501 atatttaaat attaaggagg acttatggga aatagatctg ataaaccaat gaatccatta
     4561 gaaatgcttg catatatttt atcaaagcaa attaaagatc atgaagtagt ttatattggg
     4621 acgggtttgc caatgattgc tggtatactg gcacgcaaga cacatgcccc taacatagtc
     4681 ctggtatatg agtcaggtgc acaggaccca atggaaggag cacttccatg gtcggttggt
     4741 ggtccagcaa catggcgcaa gtcgccgtta ataatggaaa tggcttattc atttggccag
     4801 ctatataatg gctatgttga taaagcattt ttgggttttg cacagattga catgtatggc
     4861 aatgtcaata cacacatgat aggtaaagat ttcttgaaac cagatcaccg cttgactggt
     4921 tcaggtggga ataatgacct ttcttcactg gctgaaaata tgattcttgt tggattgcaa
     4981 tcaccacaaa aatttgttcc caaagttgat tttattactt caccgggatt tctagatggt
     5041 ggcgatagca ggaagaaagc agggttactg ggcaatggac ctgtatgcgt tatttctcag
     5101 gctgcggtat ttgattttga acctgaaaca aaacggatga gagtcaaatc attgcatccg
     5161 ggagtatcat ttgatctatg ccagttagct tgtggatttg aattgctgaa acctgaaggg
     5221 gaaatccctg taactgaagt tccatcaaag gaaattcttg aaatattgcg aaatgaagtt
     5281 gatccacgcg gagtgtttac aagcattcca ggaatgtagg tacttggtat aagaaattta
     5341 aaagaaaacc tctgtacatc aaaacagagg ttttcttttt ccctacagga tggatgttat
     5401 atatgtgcag tttcagtaag cttctcattt ccagagatgt gaaaagatta tggcagtatt
     5461 caatactctt cgagtgcatt caatgaccgg ctttaagaca ccaggtcacc cagtgttctg
     5521 agcaaagcaa agaatgtatc aacacggcat cgatagctga cgctcatatc aatgacagtg
     5581 ttattggtaa aaaattaata tattttttat ttccctaatg ccagtttaat aacttcgtac
     5641 gctccattgt acacaccaat actctttgcc ttgataatgt tattgcagta cattgaaaga
     5701 aggtcatcat catacatcaa caaatcaagt gcctgcaatg tatgctcggt tgtttcgcat
     5761 tcaacggtac tatcgccaac ttcagcacca cgtattgcac cccacatttc atgaccacca
     5821 acccttgcaa caaaaagctt tggtactggg taaaatgcca gttcacttgg ctttgtgata
     5881 aggcagtccg acactcgcat taaaagattt gtggcataca cagcggaaaa tatattttca
     5941 ttatatacca catgaagtcc tggaatatcc tttgcactca aggtgtttac caatttttct
     6001 gtatctgtcc actgagtgta aattttacat tgaatttttg ggctaaagtg tgtattgact
     6061 atcatatcaa aaatgttttt atggtcaccg gtatttataa taagtgtgag tttttcattt
     6121 tccagtagcg gtatgcaatg ctgtatgatg tccatacaca gtttttgctg tgcgccagcg
     6181 ccaccaatgc ttaataaaaa tcggcgtggc ttttttgctt tcattctgtt tatgcgtgca
     6241 gtacagtctt tttcaatatt ggcaaccagt tcgtggtcaa tataatggcc tgtataatac
     6301 aaactgtctt ttggcatagg atttaagatt tcattgcgct tgcccatatt ttttaatgtc
     6361 ctgtatccat aataggcaga tggtgtctgc acggtgtgta gagctccttc ggaaagatgc
     6421 agtgccagag gccagttgtc gggtatcatg ttgacaacac gtttcatacc agcatgaatg
     6481 gcagcctgag atgcccatgc atgtgtagca acaaaaggca cagagtgggg aagattttcg
     6541 tatatatttg caaaaagctc ggtcattttc tggtcactgg cattataggg caggcgttta
     6601 aacccaattg cggtgagatg ctcctagtac aatttattaa acaaataaaa ttgctgtgaa
     6661 aggcgggagc ccagtgaata cagcttttct aaatgtttga tgattttggc accggtagta
     6721 tcaaatgaaa gaaggtcaaa ccagtatggt gttaacccca tggaatgtgc tgctgatgca
     6781 actgccatag ctatacggta atgaccataa cccatgcgaa tagttccaat gatgattgat
     6841 tttgattttg caatttctgc acccttttcc tgcgaaatgg tgtacaggtt aacatatttt
     6901 tttaatacgg ggttcggctg agcaaaaagt gcataggagt cctgtggatt atatccaaat
     6961 tgttttgcaa aacgttcttt ctgctttacg gctttattgt acacatgttt tgaaatctga
     7021 ttaccaaaaa tagattgaga tctgtcgcct gtcatgtaac tcctccctaa aaattgaatt
     7081 cagtactgga tattcttgca aaaaattccg ccctgttgga acaacaaaac gatattatga
     7141 ataactatcg cccacatatt ttttggcgat agttactgtt aataaattca ccgaaaacaa
     7201 caaaatattg caagttttaa ttttaaaata taaaaaaatt tttaaaattg ttgacaggtt
     7261 tgctatcttt atcactatga ccgaccatat ttagtggttt tcttcctctt agatta
//
LOCUS       DAORYL010000114         6704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_7962_length_6704_cov_16.507294, whole genome shotgun sequence.
ACCESSION   DAORYL010000114 DAORYL010000000
VERSION     DAORYL010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7962_length_6704_cov_16.507294"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(591..2288)
                     /locus_tag="PLV81_14090"
     CDS             complement(591..2288)
                     /locus_tag="PLV81_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOM11268.1"
                     /translation="MILLNPKKYNVKFPDKESEDIMKKTIQFFENKGLKKVLDDDHNC
                     VWYDDFLQFQKENQIFAKLFTPKGYGDKDSHWNTWRIVNFAEILGFYGLAYWYTFQVS
                     QLGLGPIWIGKNEEVKQKAAKLLKEGAIFAFGLSEKEHGADIYSSDMMLYPNGDGTYR
                     ANGDKYYIGNANKAGLVSTFGKIADTGDYVFFVVDSQHPKFECIKNVIHSQSYVAEYA
                     LHDYPITEADILDKGRDAWDASLNTINICKFNLGWGSIGIATHCFYEALNHAAHRNLY
                     GKYVTEFPHIKQFFVDAYTRLVAMKLFALRATDYMRSANENDKRYLLYNPMVKMKVTT
                     QGEEVINLLWDIIAAKGFEKDTYFHMAARDIRALPKLEGTVHVNMALIIKFMFNYFFN
                     PKEFPEVPQRLDATNDDFLFNQGPTKGLGKIQFHDYNIAYNSVNLPNVEIFKKQINAF
                     KDFMMGATPDQKQAEDIDFLLSIGELFTLVAYGQLILENKKFYNVEDDLIDQIFDFMI
                     RDFSKFALQIYSKTSSTAKQQELCLKMIQKPVVDHDRYNRVLEKYVYSLVDEYHMNE"
     gene            complement(2476..3540)
                     /locus_tag="PLV81_14095"
     CDS             complement(2476..3540)
                     /locus_tag="PLV81_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOM11269.1"
                     /translation="MKRLCTIKAGKRAYRIIKDEGLKKERIQCVLGAAGGPKWLILYG
                     LDKLVYSHFLQKRKTPVHLIGSSIATWRFAALAQKDGAKALDTFKERYMAQKYSLNPD
                     REEITAETVAIMETYLPDSRVKEIMQHPVIRLCIITARSKHLLTNERKLPLMAGLALA
                     ALSNAVSRKLLALHFDRYVFYDERQFPSFIQWNNFSTQYIPLDASNVKKAIMASGAIP
                     LVMQGVPYSYNGTATMLRDGGLVDYQMDLPLTLDGGIALYPHYSEHIVPGWFDKALPH
                     RKPQNTLDDICMVSPSPDALDLLPYKRIPDRHDFYTFAGNDAERLSFWEKALEVGQKM
                     ADEFAELVESGKIKDRVVLL"
     gene            3794..4681
                     /locus_tag="PLV81_14100"
     CDS             3794..4681
                     /locus_tag="PLV81_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11270.1"
                     /translation="MLLSPDDSILFFKLYFSLLLYANSNFKILENIYSANDIRQSDPN
                     NIIKIREQLYNHPELFDLFIHENPFKFSNNELSIIRSWKQFIKKKFIIFRYAKNYTIF
                     IDDTSDDPKAYGVLAITSPLHEMVGSQLPKMVEAVLLPFKNCIIYDSILIPYSIHFGS
                     NIRKVLNNVYQIAKSSYGIITSLPFEPKVKQSDADKLKYYLKSKHNRELYSNEIRTLI
                     NKDQGLLILFHQEMNKIDSKMYRKLLRNIGIDKGWFAMLEGIIIASSITKESLEQVVE
                     QIVPEKKRKFVYIFQLKGN"
     gene            complement(4766..5398)
                     /locus_tag="PLV81_14105"
     CDS             complement(4766..5398)
                     /locus_tag="PLV81_14105"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MjaI family restriction endonuclease"
                     /protein_id="HOM11271.1"
                     /translation="MPKEWILNQANMRWGLTKKKNVGPVSELIRKCAPKTREEWEIYY
                     LSNVYPKEHLEYLGKTLFIKVTDICKAEIEGITEDDCINFIYNLVINRTFDGYQSEIQ
                     TIYGQLENILNIKIEPAPDEWDRGYNVDYFIKIKNKYIGLQIKPAGYEYITQIINERE
                     QQKKTHEKFTQKYGGKVFYIISLTEGKNKRIHNTEVIEEIKKEILRLENE"
     gene            complement(5377..6624)
                     /locus_tag="PLV81_14110"
     CDS             complement(5377..6624)
                     /locus_tag="PLV81_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOM11272.1"
                     /translation="MNIKVIIGDSRKMPEVPDDSIGLVVTSPPYWHIKDYGTKGQIGY
                     GQTLHEYLKDIYRVWNECYKKLLPGRRLCINIGDQFARSIIYGRYKVIPLHAEIISQC
                     EEIGFDYMGSIIWQKKTTMNTTGGANVMGSYPYPPNGLIEIDYEFILIFKKPGNGNNA
                     SKESKKDSALSKEEWKTYFTGHWNFGGAKQIGHEAMFPEELPLRLIKMFTFVGDIVLD
                     PFLGSGTTIKAATQLKRNSIGYEINEDFLEIIRKKIDSSNLFDSDKIEIIKRESNIII
                     EDIEYIPKIKNAKPNIEPSKFNFKNGSFYKIVEIIGPDTIRLNSGLLVKFLGVKIIDE
                     KKALEYLKENLLKKEIIIKFDKYKKSTSEPVEAYVYLKNKIFINAYLIKSGMAVAEKN
                     NDYSYKDKFLKLENIELCQKNGY"
BASE COUNT         2136 a         1255 c         1026 g         2287 t
ORIGIN      
        1 ataatacagc ttgttaaact ctatcagcac tgtaagatca atgtaatttt tttaactggt
       61 acaactgaaa gagctaatca aattattatt gacatactgc aagttcgttt ttattatcaa
      121 tataactatt acggtatatt ctttcctttt ggatttaagt ataaaacagt atgccatata
      181 tgaatcactc aatacaacca catatcataa agaaacatat aaaagattgt aaacccactg
      241 ataattacaa ttggtggata accaaaaccc ctgaagaacg catcagtgcc gttgaaatac
      301 tgcgcaggca aatgtatgga aatacatcaa gattacaaag agttataaag atatcctcct
      361 ttaagaattg atttaatcac ttctattact ggtgttagct gggatgaagt ctataataat
      421 gcagttacag gaaattatgg tgatgtaaaa gtacttttta ttgggaaaca gcagtttatt
      481 aaaaacaaaa aggcattagg tagatatcag gatttggctg atttagagtc aattggatat
      541 aaaaacgagg atgcagatag tccataacta tcgcatcccc gttataatct ttactcattc
      601 atatgatatt catcaaccag cgaatacaca tatttttcaa gcacgcggtt atacctatca
      661 tggtctacca caggcttctg tatcatcttt aagcaaagct cctgctgctt tgcagtactg
      721 cttgtctttg agtaaatctg cagtgcaaat tttgaaaaat ccctgatcat gaaatcaaaa
      781 atctgatcaa tcaaatcatc ttcaacattg tagaacttct tgttttctaa aataagctga
      841 ccatacgcaa ccagggtaaa caactcacca attgacaata aaaagtcaat atcttcagcc
      901 tgtttctggt caggggttgc acccatcatg aaatccttga atgcgtttat ctgcttcttg
      961 aaaatctcaa cgttgggaag gttaacgctg ttataggcaa tgttgtaatc gtggaactga
     1021 atcttgccca atccctttgt tggtccctga ttgaatagga aatcatcgtt ggtagcatct
     1081 aatctctgcg gtacttcagg gaattcctta ggattaaaga agtagttgaa catgaacttg
     1141 ataatcagcg ccatgttaac atgaactgtt ccttctaatt ttggcaatgc cctgatgtcg
     1201 cgagcagcca tgtggaagta tgtatccttt tcaaaacctt tagcagcaat gatatcccat
     1261 agcaagttaa taacctcttc accctgtgtg gtaactttca ttttaaccat tggattgtag
     1321 agtaaatacc gcttgtcatt ttcattagcg cttcgcatat agtcagttgc acgcagagca
     1381 aacaatttca ttgccacaag ccgtgtgtat gcatcaacaa agaactgctt gatgtgcgga
     1441 aattcagtaa cgtatttgcc gtacaggtta cggtgtgcag cgtgatttaa cgcttcataa
     1501 aaacaatggg tagcaatacc aattgaaccc cagcctaaat tgaatttaca gatattaata
     1561 gtattgagcg atgcatccca ggcatccctg cctttatcaa ggatatcagc ttcggtaata
     1621 ggataatcgt gcagcgcata ctcagcaacg tatgactggc tgtgaataac gtttttaata
     1681 cattcaaatt ttggatgctg tgaatctacc acaaagaaca catagtcgcc cgtatcagca
     1741 attttgccaa atgttgacac cagcccggct ttgttggcat tgccaatgta gtatttgtcg
     1801 ccatttgcac ggtaagtacc atcgccattg ggatacagca tcatatcact tgaatagatg
     1861 tcagcgccat gttccttttc ggacaaacca aatgcaaaga ttgcaccctc ttttaaaagt
     1921 ttggcagctt tctgcttaac ttcctcgttt ttgccaatcc atattggccc aagaccaagc
     1981 tgcgaaacct ggaatgtata ccagtaagca agcccataaa atcctaaaat ttcagcaaag
     2041 ttgacaatgc gccaggtatt ccagtggctg tccttatcgc catacccttt tggtgtaaag
     2101 agtttagcaa aaatctgatt ttccttctga aactgcaaaa agtcatcgta ccacacacag
     2161 ttgtgatcat catccagaac cttttttaat cctttgtttt caaaaaactg tatggttttt
     2221 ttcataatgt cttccgactc tttgtcggga aatttgacat tgtacttttt tggatttaaa
     2281 agaatcatat cccaccccta ttcaaattat agtaacaaat ttgagtatat catgtctgta
     2341 ctgatttgta aagaaaaaat tacagtgtta tgtatttttt taatcaacta ttttttatat
     2401 ttgtcaatgt gaattatggg cgtgcccccg ccgtacatac gcctcaattg gcacatcatt
     2461 aattccaatg catcactaca ataaaaccac cctgtcttta atcttcccgc tttccaccaa
     2521 ttctgcaaat tcatccgcca tcttttgtcc cacctcaagt gctttttccc agaatgataa
     2581 cctttcagcg tcattgcctg caaacgtgta aaaatcatgc ctgtcaggaa ttcttttgta
     2641 gggaagtaaa tcaagcgcat caggcgacgg gcttaccata catatatcat ctaaggtatt
     2701 ttgtggtttg cgatgcggca acgccttatc aaaccatccc ggcacaatat gctcagagta
     2761 atgtggatac agcgcaatgc caccatcaag tgtaagtggc aggtccattt gataatcaac
     2821 aagtccgcca tcgcgtagca tggtggcagt gccgttgtag gaatacggaa ccccttgcat
     2881 aaccagtggg atggcacctg aagccatgat ggcctttttt acatttgatg catcaagcgg
     2941 gatatactgc gtgctgaaat tattccattg aataaatgac gggaattgtc gctcatcata
     3001 aaacacatag cggtcaaaat gtaatgctaa aagctttctg cttacagcat tggaaagcgc
     3061 cgctaaagct aaacccgcca tgagcggaag cttacgttca tttgtaagta gatgttttga
     3121 tcgcgctgtg atgatacaca gacgtataac aggatgctgc ataatttctt ttactctgct
     3181 atcaggcagg taggtttcca taatggcaac agtttcggct gttatctctt ccctgtcagg
     3241 attaagcgaa tatttctgcg ccatgtagcg ctctttaaat gtatccagtg cctttgcacc
     3301 atccttctgt gccaacgcag caaagcgcca tgttgcaatg gatgaaccaa ttaaatgcac
     3361 tggcgttttt cgtttttgca aaaagtgcga atacacaagc ttatcaagcc cgtatagaat
     3421 cagccacttg ggaccaccag cagcaccaag tacacactga attctttctt ttttcaggcc
     3481 ttcatcttta atgatacgat atgcccgttt tcctgctttt attgtacaca aacgtttcat
     3541 agtatcctca gattattgga acagtatttt tgaataacag tgttatggta tggggataaa
     3601 agcaaggatt ttttggttta ttttgtactt gaatgtatac tatataattt gattattttg
     3661 gataagtact aatagaaaac cattttattc taaaaaatat aaaaaaataa ttgaaaaaca
     3721 catatataaa aattccaata ttatatgcaa taataatctt ccaatatctt taaaaagaat
     3781 atgtggagaa acaatgcttt tatcaccaga cgacagtata ctatttttta agctgtactt
     3841 ttcattgctg ctttatgcta acagcaattt taaaattctg gaaaacatat attcagctaa
     3901 tgatatacgt caatccgatc ctaacaacat aatcaagatt agggaacaat tatataatca
     3961 tcctgaatta tttgatttat tcattcatga aaatccattt aagttttcta ataatgaatt
     4021 aagcataatt agatcatgga aacaatttat taaaaaaaag tttataattt ttcgttatgc
     4081 aaaaaactat actattttta ttgatgatac cagcgatgat cccaaagcct atggcgtgtt
     4141 agcaataact tcacctttac atgaaatggt tggatcacaa ttacctaaaa tggtagaggc
     4201 agttctattg ccatttaaaa attgtattat ttatgacagt atcctaatac catattccat
     4261 acattttggt agtaatatca gaaaagtttt aaataatgtc tatcaaatag caaaatcatc
     4321 ctatggtatt attacatcct tgccatttga accaaaagta aaacaaagcg atgctgataa
     4381 attaaaatat tacttaaaaa gtaaacacaa tcgtgaatta tatagtaacg aaatacgtac
     4441 attaattaat aaagatcaag gattattaat tctatttcat caggagatga acaaaattga
     4501 ttccaaaatg taccgaaaac tattacgtaa cattggcatt gataaaggct ggtttgccat
     4561 gctggaaggc ataattattg ctagtagtat aacaaaagaa tcccttgagc aagttgtaga
     4621 gcaaattgtt ccagaaaaaa agaggaaatt tgtgtatatc ttccagctaa aaggaaattg
     4681 aatttttttt tgatgaattg ggatgtattt catacttgaa gctgcaaaga tcatctcatt
     4741 taagcatatt aaataaattg aaatattatt cattctctaa tcttaaaatc tcttttttta
     4801 tttcttcaat tacttctgta ttatgtattc ttttattttt accctcagtt aatgaaataa
     4861 tgtaaaagac ttttcctcca tatttttgag tgaacttttc atgagttttc ttttgctgtt
     4921 ctctttcatt aattatctgt gttatgtatt catagcctgc tggttttatt tgaagaccaa
     4981 tgtacttatt ctttatttta ataaaataat caacattata tcctctatcc cattcatctg
     5041 gtgctggttc tatttttata ttcaaaatat tttcaagctg accataaata gtttgaattt
     5101 cagattgata tccatcaaaa gtcctattta tcacaagatt ataaatgaag tttatacaat
     5161 catcttcagt tataccttct atttcagcct tacatatatc agtaactttt ataaataaag
     5221 ttttaccaag atattcaaga tgctctttag gatatacatt cgaaagataa taaatttccc
     5281 actcctctct tgttttaggg gcacattttc ttattaattc tgaaactggg cctacatttt
     5341 tctttttggt cagaccccat ctcatatttg cctgatttaa tatccattct tttggcataa
     5401 ctcaatattc tccaatttta aaaatttatc tttataacta taatcattat ttttttcagc
     5461 aactgccata ccgcttttaa ttagataagc attgataaat attttatttt ttagatacac
     5521 ataggcttct actggttcag aagtactttt tttatatttg tcaaatttaa taataatttc
     5581 tttttttaat agattttctt ttaaatactc caaagccttt ttttcatcaa ttattttaac
     5641 acctagaaat tttactaaaa ggccactatt caaacgtatt gtatcaggtc caattatttc
     5701 aacaatctta taaaaagaac catttttaaa attaaacttt gaaggttcaa tattgggttt
     5761 agcatttttt attttaggga tatactcaat atcctcaatt attatattgg attccctttt
     5821 tattatttct atcttatcac tatcaaacaa attactacta tcaattttct ttctaatgat
     5881 ttctaaaaaa tcctcattta tttcatatcc aatagagttt ctttttaatt gagtagcagc
     5941 cttaatagtt gtaccacttc caagaaatgg atcaagaaca atatccccaa caaatgtaaa
     6001 cattttaatc aggcgcaagg gtagttcctc tgggaacatt gcctcatgtc caatttgctt
     6061 tgcaccacca aaattccaat gacccgtaaa atatgttttc cactcttctt ttgataaagc
     6121 tgaatctttc tttgattcct ttgatgcatt gttcccattg cctggttttt taaaaatcaa
     6181 tataaactca tagtcaattt ctattaatcc attaggagga tacgggtatg aacccattac
     6241 atttgctcca cctgtagtat tcattgtagt ctttttctgc catataattg accccatata
     6301 gtcaaaacca atttcttcac attgcgaaat gatttcagca tgaagaggaa ttactttata
     6361 acgaccataa ataatcgaac gtgcgaattg atctcctata ttaatacaaa gcctacgacc
     6421 aggtaataat ttcttataac attcattcca tactctgtag atatctttaa gatattcatg
     6481 caaggtttgc ccatatccaa tttgtccttt tgttccatag tcttttatat gccaatatgg
     6541 cggtgacgta acaaccagac ctattgaatc atctgggact tctggcattt ttctactatc
     6601 acctattata acttttatat tcatcttata tccttaatta aatttagtta ttaaatgagg
     6661 gtggggattt tctcaaaaaa agtcaagaaa ttaattgact aaat
//
LOCUS       DAORYL010000115         6639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_8059_length_6639_cov_12.372114, whole genome shotgun sequence.
ACCESSION   DAORYL010000115 DAORYL010000000
VERSION     DAORYL010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6639
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8059_length_6639_cov_12.372114"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLV81_14115"
     CDS             <1..185
                     /locus_tag="PLV81_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11273.1"
                     /translation="YYNPPGGIKYCLNTKIASCELQCIFGDEQLTLKTKNRCAFEILT
                     DDADHGLQPIDTITRL"
     gene            265..918
                     /locus_tag="PLV81_14120"
     CDS             265..918
                     /locus_tag="PLV81_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11274.1"
                     /translation="MKRTIATIIFIAIAVLILCSTGSSQELSPIIKIYPFAPGDNFLI
                     NWNWITALDYKKISDWGKVYHCTVNYDDRPVIDNCAWFISKYGSRLIFNNLTESTYYL
                     HINFVTFDQPEKTPIDALCIIYCNNSPVKQLAFREMTATNSMVVIKITREFIVNKTLT
                     VEFKEYSSAGGFFGVWDVLLSRLDTLPDAIEMKKSDSEQKTMKQAPAKLVKPKSIKK"
     gene            1156..1440
                     /locus_tag="PLV81_14125"
     CDS             1156..1440
                     /locus_tag="PLV81_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11275.1"
                     /translation="MPNDFEKEFDDEVYEDDFYEETFDDEEILEEEMLEDDLIEEDEV
                     EEDEKQIAVILACDDCDYRWEDVIIKHKDEDIEDLEIICPMCGSTNITQI"
     gene            1437..4262
                     /gene="uvrA"
                     /locus_tag="PLV81_14130"
     CDS             1437..4262
                     /gene="uvrA"
                     /locus_tag="PLV81_14130"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOM11276.1"
                     /translation="MSQQYIRIKGAREHNLKNISIDIPREKLVVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMEKPDVDLIEGLSPAISIEQKTTHRNPRSTVGT
                     VTEIHDYLRLLFARVGVPYCYKCGKPITSQTIDQIVENVLTHTAGTKIQVLAPVVRGR
                     KGMYQDMLEKFKQQGYSRVRVDGQVYSLDDTIKMDKNKKHSIEVIIDRIVIKENIRSR
                     LSDSIETAIELADGQVILIIDDNEQLISTKMTCPDCDVSLPELSPRMFSFNSPYGACP
                     TCDGLGFIMQFDPELIVANPDKSLMDGALAVWGKTTSYWYQEQVLTLARYLKFDPHTP
                     WKKLPKQIQNIVLYGSNGKKFDYSIERFEAQYRFTKEFEGVIPNLMRRYKETQSDEMR
                     EWMEGFMRNRVCDACGGKRLKPESLAVKVADKNIADVSAMSIKEAVKFFKDISLSETQ
                     MTIARQILKEIRARLQFLSNVGIDYLTLDRAAGTLSGGEAQRIRLATQIGSGLTGVLY
                     ILDEPSIGLHQRDNKKLLDTLKELRDIGNTVIVVEHDEETIRESDYVIDLGPGAGLDG
                     GYVVAHGTPQEIEQNETSITGLYLSGKRYIKAPLKRRVAEKYLTIRGAAENNLKSIDV
                     SFPLGVFTCVTGVSGSGKSTLIIDILYKALASLKMRSKDYPGKFKKIEGIEYIDKVID
                     VDQSPIGRTPRSNPATYTGTFTPIRELFASLPESKIRGYKPGRFSFNVSGGRCEACWG
                     DGIKKIEMHFLPDVYITCEVCKGKRYNHETLEVRYKGKNIYEVLEMTVDEAYEFFINI
                     PSIKSKLETLKRVGLGYIKLGQPATTLSGGEAQRVKLSTELSKRATGNTLYILDEPTT
                     GLHFADVEKLIDVLQSLVEKGNTVIVIEHNLDVIKCADWIIDLGPEGGDAGGRVIAQG
                     TPEDVAKVAGSYTGQFLTQILSRKYKS"
     gene            4634..>6639
                     /locus_tag="PLV81_14135"
     CDS             4634..>6639
                     /locus_tag="PLV81_14135"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HOM11277.1"
                     /translation="MYKNIITYIIIIIIMTFIPYGGFSLDKQVAIFQKAKSEVAKGMY
                     HFNEMHYLAAIEFFRKALEIYPDYYTANEYLARAYKLAGYTNLALQQWQILLDMTKEN
                     PLIQNKIDMINFRGSIYSSLEHPFELIETAVYLPVDLGRFRFSSPIDIAIDNDKNMYI
                     TSFSNGKLVKLDPNGEGIFTRTYNLDGKLYGIDYRSGLLVVSDFANNKVFIINTDGKI
                     VKTIGRTGNTEGQFNGPEGVCFGGDSSLYVVDSGNHRVQKFGLDGRFILAFGQFGEYE
                     GQLNKPTDVAVRYENVYVTDTNNKRIAVFDDSGNFVENLTPSEFAVPRGIFIQGNLMA
                     VSDEKKGLFMYNFDTGQSQWFVSWEGKKKFYHLTSAVMDDNGFVYTCSNKSEAIYVFS
                     PLQQHISNIEVEVTNVDAKKFPTVAVYCNIRDRYGRPIYGLTKENFTIIEDGATITNL
                     SADYLKNMMPAASMVMCVDRSGSLKNYHNDVPWLAEFILQKMHKNDQLKIINFADDTW
                     VANPYDWSRRRALKALKTFDYGKGRDIGKALYTAISDVLPQMSRRGVILITDGDANEN
                     DFKTYSPDIIIDYAKSHFIPVYILTLKTKSPILMRIAQETGGAIYKGSELDGLRTVYD
                     AIKKANDYRYVLIYSTFKMKSLKGWWSDLTVNVSYKGQKGTEWAG"
BASE COUNT         2188 a         1049 c         1389 g         2013 t
ORIGIN      
        1 catattataa tccacctggt ggaataaaat attgccttaa tacaaagatt gcttcgtgtg
       61 aattgcaatg tatttttggt gatgagcaat taacgttaaa gacaaagaac agatgtgcct
      121 ttgaaatatt gacagatgat gcggaccatg gcttacagcc aatagatact ataacacggt
      181 tatgactaga gtaattatat tgtcgtaaat taaagtaata aaattttctt gcaattgttg
      241 tgtgattttg aaactattgt gataatgaaa cgcacaatag caacgattat ctttatagca
      301 atagcagtgc ttatattgtg ttccacaggc tcttcacagg aactatcgcc tattataaaa
      361 atatatccgt ttgctcccgg ggataatttt ttaattaact ggaactggat aaccgcttta
      421 gattacaaaa aaatttctga ttggggtaaa gtatatcatt gtaccgtcaa ttacgatgat
      481 aggccagtta ttgataactg tgcctggttt atttcaaaat atggctccag gcttatcttt
      541 aataatttaa ctgaaagtac atattacctg catataaatt ttgtgacatt tgatcaacct
      601 gaaaaaacac caattgatgc tttgtgtatt atatactgta ataacagtcc tgttaagcaa
      661 cttgcattca gagaaatgac agcaacaaac agcatggttg ttattaaaat aacgcgtgaa
      721 tttattgtta acaaaacctt aacagtggaa tttaaagaat attcttcagc aggtggtttt
      781 tttggtgtgt gggatgtact tttgagcaga ctggatacat tgccggatgc tattgaaatg
      841 aaaaagtcgg attcagaaca aaaaactatg aagcaagcac ctgcaaagtt agtaaaacca
      901 aaatctataa aaaaataact ttttttattg accatttttc atatactaac ttttttgaca
      961 aaaaggtatg gcaatttttt aaaaattttt gcatgagttg tgcactacat ttgtattgta
     1021 gtgtaaaaac tgtgtaatca ggaaatgcct taaaatttta tgtttttttt aaaattttgt
     1081 tagatagttt ttaaagtatt catatattga tagtagtatt ttgcataata atatcagtat
     1141 aagagggtgt agattatgcc aaatgatttt gaaaaagaat ttgatgatga agtatatgaa
     1201 gatgatttct atgaagaaac atttgatgat gaagagatac ttgaagaaga gatgcttgaa
     1261 gatgatttaa tagaagaaga tgaagtggaa gaagatgaaa aacagatagc tgtgatttta
     1321 gcctgcgatg attgtgatta ccgatgggaa gatgtaataa taaaacataa agatgaggat
     1381 attgaagatc tggagattat ctgtcctatg tgcggttcga ctaacattac acagatatga
     1441 gtcagcaata tattcgcatt aaaggtgccc gtgagcacaa tttaaaaaac atttctattg
     1501 acattccaag ggaaaagttg gttgttatta cagggctttc cggttcaggg aagtcctctc
     1561 tggcttttga taccatttat gccgaaggtc aacggcggta tgtagagtca ttgtcggcgt
     1621 atgccaggca gtttttaggt caaatggaaa aacctgatgt tgacctcatt gaagggcttt
     1681 cacctgcaat atccattgaa cagaaaacca ctcaccgtaa tccccgttca acagtaggta
     1741 ctgtaaccga aattcacgat taccttcgtt tgctctttgc acgtgttgga gtgccatact
     1801 gttataagtg cgggaaacca ataacctcac aaaccattga ccagattgtt gaaaacgtac
     1861 ttactcatac tgccgggaca aaaatacagg tacttgcacc agttgtgcgt ggtcgcaaag
     1921 gtatgtatca ggatatgtta gaaaaattca aacagcaggg atactcacgt gttagagttg
     1981 atggccaggt gtattcgctt gatgatacca taaaaatgga taaaaataaa aagcattcta
     2041 ttgaggtcat tattgacagg atagttatca aagaaaatat acgttcacga ttatcagatt
     2101 caattgaaac agcaattgaa ttagctgatg gtcaggtcat attaatcata gatgataacg
     2161 aacagttaat atccacaaaa atgacctgtc ctgactgtga tgtttcattg ccggaactat
     2221 caccacgcat gttttctttc aatagcccct acggtgcatg tcccacctgt gacggattgg
     2281 gttttatcat gcaatttgac ccagagctta ttgttgctaa tcctgataaa tcattaatgg
     2341 acggagcact tgctgtgtgg ggaaaaacaa caagctactg gtatcaggag caggtgctaa
     2401 cactggcacg gtatcttaag tttgatcccc atacgccatg gaagaagcta ccaaagcaaa
     2461 ttcagaacat tgtgttgtat ggctcaaatg gtaaaaagtt tgattattcc attgaacggt
     2521 ttgaagccca atatcgtttt acaaaggaat ttgaaggtgt tatcccaaat ttaatgcgtc
     2581 gctacaaaga aacacaatct gatgagatgc gcgagtggat ggaaggcttc atgcgcaatc
     2641 gtgtgtgtga tgcgtgtgga ggtaaaaggc taaagcccga gagtttagca gtaaaagttg
     2701 ctgataaaaa tattgccgat gtaagtgcaa tgtctattaa agaagcagta aaatttttta
     2761 aagacatttc cctgtcagaa actcagatga caatagcccg ccagatactt aaagaaatac
     2821 gagcacgatt gcaatttctt tccaatgttg gcattgatta tcttacattg gaccgtgcag
     2881 ctggtacatt gtctggtggt gaggcacagc gcatacggct tgccactcag attgggtcag
     2941 gtttaacggg tgtactgtat attcttgatg agccaagtat tggccttcat caacgtgaca
     3001 acaaaaagct tttagatacg ttaaaagaat tgcgggatat tggaaacacg gtaattgttg
     3061 tagagcatga tgaagagaca attcgcgaat cagattatgt cattgattta ggtcctggtg
     3121 cagggcttga tggtggttat gtggtagcgc acggcacacc ccaggagata gaacaaaatg
     3181 aaacatcaat tactggatta tatttaagtg ggaaacggta tatcaaagca ccgttaaagc
     3241 gcagagtggc tgaaaagtat ttaaccattc gaggtgcggc ggaaaataat ttaaaatcaa
     3301 tagatgtgtc atttccttta ggtgtgttta cctgtgttac tggagtttcc ggttcaggaa
     3361 aatctacctt aataattgat attttgtata aggcgctggc ttcattgaaa atgcgcagta
     3421 aagattatcc cggtaaattt aaaaagatag aagggataga atatattgat aaagtaattg
     3481 atgttgatca gtcaccaata gggaggacac cacgatccaa tcctgctaca tatactggaa
     3541 catttacgcc aattcgtgag ctttttgcaa gcttacctga atcaaaaatt cgcgggtata
     3601 agcctgggag gttttcattc aatgtatcgg gtggcaggtg tgaagcatgc tggggtgatg
     3661 gcatcaaaaa gatagaaatg cattttttac ctgatgtgta tataacctgt gaagtgtgta
     3721 agggtaaacg ctataaccat gaaacattag aagtgcgtta taaagggaaa aatatatatg
     3781 aagtactgga aatgactgtt gatgaagcct atgaattttt tattaatatc ccatcaataa
     3841 aatctaagct tgaaacattg aaacgggtag ggcttggata tatcaaattg ggtcagcctg
     3901 caacaacgct gtcaggtggt gaagcccaac gggtaaaatt atcaaccgag ctatccaaac
     3961 gggctacagg aaacacactg tatatccttg atgagccaac tactggattg cactttgcag
     4021 atgtggaaaa acttattgat gtattacaga gtttagttga aaagggcaat actgtcattg
     4081 ttatagagca taacttagat gtcattaaat gtgctgactg gattattgac ttagggccag
     4141 aaggtggaga tgctggaggg cgtgttattg cccagggtac accagaagat gtggcaaagg
     4201 tagcaggttc atatacagga caatttttaa cacagatttt atcacgtaaa tataaatctt
     4261 aagtaatatg tgcactttct tttgtttggc gacaaaacaa agtgccaaag aaaaatgcct
     4321 ttttgtcttt aggcatataa taatctttct atagcaggct gcggcataga gtccattcac
     4381 gtccgcgccc acatcccatt gcaccttgca tccctgcaag gctgcttaat gtcatacttt
     4441 ttgataaaac acaaattttt aatattaaaa gtatttaaat tatttgcaat tatttctata
     4501 atatacgtaa tatgccaatt ataccgataa ataaataaat ctggttttct atgtattgat
     4561 tttgtaatgt taagggaaaa atcaatgtat aagggtttat taatgattgt tactaaattg
     4621 tagaggttta tccgtgtata aaaatataat aacttatata ataataatta ttattatgac
     4681 gtttatccct tatgggggct tctcgttaga caaacaggtt gctattttcc agaaagcaaa
     4741 aagtgaagtg gcaaaaggaa tgtatcattt taatgaaatg cattaccttg ctgctattga
     4801 atttttcagg aaagcgctgg agatttatcc tgattactat accgcaaatg agtatttagc
     4861 acgtgcatat aaactggctg gttatacaaa cctggcacta cagcagtggc aaatcctgct
     4921 tgatatgacc aaagaaaatc ctcttattca gaataaaatt gatatgatta atttcagagg
     4981 ttcgatatac agttctcttg aacatccatt tgaacttatt gaaactgcag tatatttacc
     5041 tgttgattta ggaaggttcc gtttttcctc acccatagat attgccattg ataatgataa
     5101 aaacatgtat ataacatcct tttcaaatgg aaagcttgtc aagcttgatc ctaacggtga
     5161 aggcattttt acacgcacat acaaccttga cggcaaactc tatggcattg actacaggtc
     5221 aggtctttta gtggtaagcg attttgcaaa caataaagtt ttcattatca acaccgatgg
     5281 caaaatagtt aaaacaattg gcagaacagg caatacagaa ggccagttta acggccctga
     5341 gggtgtttgt tttggcggcg atagttcctt atatgtggtg gacagtggca atcatcgtgt
     5401 acaaaagttt ggtttagatg gacgattcat cctggcattt ggacaatttg gtgaatatga
     5461 aggtcaactg aataaaccaa cagatgtagc tgtgcggtat gaaaacgtat atgtgacaga
     5521 tacaaacaac aaacgaatag ctgtttttga tgattcaggt aattttgttg aaaaccttac
     5581 accatcggag tttgctgtac ctcgaggaat ctttattcag ggcaacctca tggctgtgag
     5641 tgatgaaaaa aaaggtcttt ttatgtataa ctttgataca ggtcagtcac agtggtttgt
     5701 tagctgggaa gggaaaaaga aattttatca tcttacttcg gcagttatgg atgataatgg
     5761 gtttgtgtat acatgcagca ataaaagtga agccatatat gtgttttcac cactccagca
     5821 acacataagc aatattgaag ttgaagtaac taatgttgat gcaaaaaagt ttcccactgt
     5881 ggcagtctat tgcaatattc gtgaccgtta tggtagacca atatatggat taacaaaaga
     5941 aaacttcact atcattgagg atggagcaac aatcacaaat ctaagtgctg attatcttaa
     6001 aaacatgatg ccagcagctt caatggttat gtgtgttgac cgttcaggtt cattgaagaa
     6061 ctatcataat gatgtaccat ggcttgcaga atttatattg cagaagatgc ataaaaacga
     6121 tcaactgaaa ataataaact ttgctgatga tacctgggtt gcaaatccgt atgattggag
     6181 caggcgaagg gcattgaaag cattaaaaac atttgactat gggaaagggc gtgatattgg
     6241 caaggcactg tatactgcaa tcagtgatgt attgcctcag atgtcacggc gtggcgtaat
     6301 acttattaca gatggtgatg caaacgaaaa tgatttcaag acctattccc ccgatattat
     6361 tattgattat gcaaaatccc attttattcc agtgtatatt ctcacattaa aaacaaagtc
     6421 gccaatttta atgcgtatag ctcaggaaac aggcggagca atctataaag ggagtgaatt
     6481 agatggatta cgcacagtgt atgatgctat aaaaaaggca aacgattatc gctatgtatt
     6541 aatttattca acgtttaaaa tgaagtcttt gaagggatgg tggtcagatc ttactgtcaa
     6601 tgtttcatat aaagggcaaa aaggaaccga atgggctgg
//
LOCUS       DAORYL010000116         6595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_8129_length_6595_cov_5.769572, whole genome shotgun sequence.
ACCESSION   DAORYL010000116 DAORYL010000000
VERSION     DAORYL010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6595
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8129_length_6595_cov_5.769572"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..825)
                     /locus_tag="PLV81_14140"
     CDS             complement(247..825)
                     /locus_tag="PLV81_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11278.1"
                     /translation="MGIVIDINDFLHYGNVKYNLAHADKMLFDRCQAAICATTNLIKG
                     EAIVTDISCKLFTDNPDCKGTVTVLITHVPNQIHWKCPACGSEGIIKNWKNSPLYIQF
                     LKDSNQKSKQIKSITLSSEEFEKLVNLSDGLDECKAMIASAQKTGTSYTISVNTINVF
                     QILEKLIFKIELKTPDRPFYIKLRDELKTAVI"
     gene            complement(894..2255)
                     /locus_tag="PLV81_14145"
     CDS             complement(894..2255)
                     /locus_tag="PLV81_14145"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOM11279.1"
                     /translation="MYTVTGNVVQKLRSKKHYTNWQEEYEDKLCSADQAAALIKSGDR
                     IAMSGGTSIPYEFSRALSKRAGQIKNVGISMGYAMALFDYMKPENHESFYLETVFVGP
                     MERICMDWGVCQYVPIHLGETANYCRASRLNIAVARVTPPDENGFMCRSNFASFLDLD
                     TIREADYVIVEVNKHMPRLVAPEFKIHVSEVDYIIEHHEPIFEIPNIEITEAEQKMAD
                     YISEMIPDGATVQLGFGGLPNAIGYNLRNKKHLSIYSEVATPAMKDLIECGAVDGSMR
                     TFMQGRKVVAGFCVGTRDFYDYVENNQDFEFKLIGFINNPLNIAKNDNIVAINNALML
                     DLTGQVASESIGAKQYSGTGGQVQFILGAKFAKNGKNILAINSTFTDKDGKLRSKIMP
                     SFPENTVVTTSRNDVEYVVTEYGVAYLKYKSIYQRAKSLIAIAHPDFRDELKFEAKKR
                     KWL"
     gene            complement(2597..3262)
                     /locus_tag="PLV81_14150"
     CDS             complement(2597..3262)
                     /locus_tag="PLV81_14150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOM11280.1"
                     /translation="MKGTMQSNKRYKIFIVEDHPIFRDGITQLINKEDDMFVVGGCEN
                     AEECLKYLKNNQPDCIIVDITLKDSCGIELTKEIKKSYPSLPVLILSMHEELIFAERV
                     FKAGARGYITKREATSKVIDAIRRVLQGKIYICDTMIDYFLERSVTGGQNIGSSPVEK
                     LSEREFEVFNLIAKGMTNKNIARVLSVSTNTISTYRERIKEKLNLQNNAELNRYAMQW
                     MQK"
     gene            complement(3237..5441)
                     /locus_tag="PLV81_14155"
     CDS             complement(3237..5441)
                     /locus_tag="PLV81_14155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOM11281.1"
                     /translation="MNTDCLKQHTVHNSFKDIVDLSPEAIFVYVDETIVFANATALSL
                     LKATRPDDVIGRSVYSFLQPQSHNELRKVISLVLENPKSYISSEDKIIACDSENIDIE
                     ISSTYINFEGRDGVIFFFKDIKNRKKAEESLKKTELQYKTIFESTGTAMLFIEEDKTI
                     SLVNGECEKISGYTRNEIEGKMKWPFFVAPEDRWILEKNHELRRSGKSLPNNYEFRLI
                     DKTGRYHNVFMTITIIPGTKKSVASIIDLTELQEAEEILLATQHNYRLLVENSKEAIA
                     IIQDELVRYVNPKLCEITGYTEEQLLNKKFYKLVHPDFKDIVRNEYKYRIEGKEKYTL
                     FPIKIIDTHSQERWFHVNSVLTIWYQKPAVLLFFNDITQIKNAQDALMASEERYRLVA
                     ENAKDIIIIYDDHGYIHYMNESGYQTFGIKKGALSTRKINIADLLHRTSETRPPWQAI
                     EEAIKNNTSFKNRVEVFNSHGELIQLESISSPIKLDGDSSGMLVVARNITERKKLEKE
                     IIAISERIRQQVSRDLHDDLNPHLIGIEALTKVLSMRLKSLQLPEAEQADKIATLIHK
                     AITKTHRLAKGLCPIDLESTGIQSPIINLLKLIKNLYGIDGKFYFDESVIIHDPNVAS
                     NLYYIAQEASFNAAKYSEGSLIVIKLHKLENYLALSIEDNGKGIKRQRISEDDNTGGM
                     GLKIMKYRAEIIDGTIQIQNNKPSGTIITVKIPLERITDDGRVLNERNHAKQ"
     gene            complement(6041..6283)
                     /locus_tag="PLV81_14160"
     CDS             complement(6041..6283)
                     /locus_tag="PLV81_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11282.1"
                     /translation="MPAFVKYHSQLHQLMANYKGQILTTNQIISIFQRYYPNADIRYL
                     QPSDHCINKTNNGACDCAMTPNAIFEYISRGKYRVI"
BASE COUNT         1914 a         1175 c         1226 g         2280 t
ORIGIN      
        1 aacatagcaa taattttata ggaaatccaa aatagtgggg tcagagcatg aattggcaat
       61 tggcaattac cggcgcaaaa gcgcatgagc tcctgcgcag aaaagaacaa attgggtttg
      121 acgtggagaa cgaagagtgc gctaagtgcg cttataagac agggtcagag ctgcatgcac
      181 tgaaactctg tggtttcagt ggagtctgca tgagggaaat ttttttaaaa attaatatgg
      241 agagatttat ataacagcag ttttcagttc atctcgcaac tttatataaa aagggcggtc
      301 gggggttttt aactcaatct tgaaaattag tttttcaaga atttggaata cattgatggt
      361 attcactgat attgtatatg aagtgcccgt tttttgcgca gatgctatca tggctttaca
      421 ttcatcaagg ccatcagaaa gatttacaag cttttcaaat tcttctgagg ataatgttat
      481 tgattttatt tgtttgcttt tttgattgct atctttcaaa aattgtatat acagagggct
      541 gtttttccag tttttgatta tgccttcaga accacatgcc gggcatttcc agtgtatctg
      601 gttgggtaca tgagtaatta atactgtcac agtacccttg caatcaggat tatcggtgaa
      661 aagtttacaa cttatatcgg ttactatcgc ttcccctttt ataagatttg tggttgcgca
      721 aatagcggct tggcaacgat caaaaagcat tttgtcggca tgtgccagat tgtatttaac
      781 attgccataa tgcaaaaagt catttatgtc aataactatc cccatatacg gtatggggat
      841 agtattatga caattttgtc accaaatttt ttacaaaaag ttagctaaat cttttacagc
      901 catttacgtt tttttgcttc aaattttaat tcatccctga aatcaggatg ggcgatagct
      961 atcagcgatt ttgccctttg atatatactc ttgtatttta aataggcaac accatattcg
     1021 gtaacaacgt attccacgtc atttcgtgag gtggtaacta ctgtgttttc agggaagctt
     1081 ggcataattt tgcttctcag tttgccatcc ttatcagtga atgttgagtt tattgccaga
     1141 atgttttttc cgtttttggc aaattttgct cccagtataa actgtacctg accaccagtg
     1201 ccgctatatt gttttgcacc aatggattca gaggctacct gcccggttaa gtcaagcatc
     1261 agtgcgttgt taattgctac aatattatca ttttttgcaa tgttaagtgg attattaata
     1321 aacccaataa gtttaaattc aaaatcctga ttattttcca cataatcgta aaaatcacgg
     1381 gttcctacac agaaacctgc aactaccttt cttccctgca taaatgttcg cattgatcca
     1441 tcaacagcac cgcattcaat aagatccttc attgctggtg tggctacttc tgagtatatg
     1501 gaaagatgct ttttatttcg caggttatag ccaatggcat ttggaagccc accaaaaccc
     1561 agctgaactg ttgctccatc aggaatcatt tcagagatat agtctgccat tttttgttct
     1621 gcttctgtta tttcaatatt tggtatttca aaaattggct catgatgctc aataatgtag
     1681 tcaacttccg aaacatggat tttaaattca ggggctacaa gtcgtggcat atgtttgtta
     1741 acttcaacga taacataatc agcctcccgt atagtatcta agtcaagaaa tgatgcaaag
     1801 tttgacctgc acatgaaacc attttcatca ggaggtgtta cccgtgcaac agcaatgttt
     1861 agccgtgaag ccctgcagta gttagctgtt tcgccaaggt ggattggcac atactgacaa
     1921 actccccaat ccatgcatat acgctccatt ggacccacaa aaactgtttc aaggtaaaaa
     1981 ctctcatgat tttccggttt catgtagtca aacagcgcca ttgcatagcc catgctgata
     2041 ccaacattct ttatctgccc tgctcgttta cttaaggcac gggaaaactc atacggaata
     2101 cttgtaccac cgctcatggc tattcggtcg cctgatttaa ttaacgctgc tgcctgatct
     2161 gccgagcaga gtttgtcttc atactcttcc tgccagtttg tgtagtgctt tttagatcgt
     2221 aatttctgta ccacattccc tgttactgta tacataagtc ctccttttaa aatagaattt
     2281 attgactcca ccacagcccc ttccttatat ttaaagaaag atattgatgc tgaggttgga
     2341 aatatttaca acagtttggt ttgagctacc ttaaacaagc tcagggacct catatcgata
     2401 caatccggga actaataata tattagttta catatattta tttaaataag aaagtctgat
     2461 aatgttgaaa ttggtgtaat gaaaagtgga tttttattag attgaaagta tgcatggtac
     2521 tgtggttatc tttaccacaa aatatgattg gaattaaaaa cagtttgtaa tgtttaaatc
     2581 aaatatagtt gtaaaattat ttctgcatcc actgcatggc ataccggtta agttcggcat
     2641 tgttttgcag attcagcttt tccttaatcc gttcacgata ggtggaaata gtattggtgc
     2701 ttactgatag aaccctggca atatttttgt ttgtcattcc ctttgctatc aggttaaata
     2761 cttcaaattc cctttcactt aatttttcca ctggtgaaga accaatattt tgacctcctg
     2821 taactgaacg ctccagaaaa taatcaatca tggtatcgca tatatagatt ttgccctgta
     2881 acacccttcg tattgcatca atcaccttgc tggtggcttc gcgcttggtg atatacccgc
     2941 gcgctcctgc tttaaaaact cgttcagcaa aaataagttc ctcatgcatt gaaagaataa
     3001 gcactggaag agaggggtaa gattttttta tttccttggt aagttcaata ccacaactat
     3061 ctttgagtgt tatatcaaca attatacagt caggttgatt gttttttaaa tatttcagac
     3121 attcttcagc attttcacat ccgcccacaa caaacatatc gtcttccttg ttgataagct
     3181 gagtaatccc atcacgaaaa attggatggt cttccactat aaatatttta taccgcttat
     3241 tgctttgcat ggttcctttc atttaaaacc ctcccgtcat cagttattct ttccagggga
     3301 atttttacag tgatgatagt tcctgaaggt ttattattct gaatctgaat tgtgccatca
     3361 attatctcag cacgatattt catgattttg aggcccattc ctcctgtgtt atcatcctcg
     3421 gatatccttt gcctttttat tccttttcca ttgtcttcaa tggacagcgc tagataattt
     3481 tccagtttat gtagctttat gacgattaag ctgccttcag aatattttgc tgcattgaag
     3541 cttgcttcct gtgcaatgta gtaaaggttt gaggcaacat tggggtcatg tataataacc
     3601 gattcatcaa aataaaattt tccgtctatt ccatacagat tctttattag tttgagcaaa
     3661 tttatgatag gggattgtat acccgttgat tctaaatcaa tggggcacaa acctttggca
     3721 agccggtgtg tcttggtgat ggctttatga atgagtgtgg caattttatc ggcctgctct
     3781 gcttcaggaa gctgtaagct tttaaggcgc atggaaagta ccttggttaa tgcttctata
     3841 ccaatgaggt gaggattgag gtcatcgtgc aggtccctgc ttacctgttg cctgatcctt
     3901 tctgaaattg caatgatttc tttttctaat tttttacgtt cggtgatatt ccgtgcaacg
     3961 acaagcattc cggagctatc gccatcaagt tttatggggc ttgatattga ttcaagctga
     4021 atcaattcgc catgactgtt gaatacttca acacggtttt taaagcttgt attatttttt
     4081 attgcttctt ctatagcctg ccagggaggg cgtgtttcgc tagtgcggtg aagcaggtca
     4141 gcaatattaa tttttcttgt gcttaaagca ccctttttaa taccaaatgt ttgataacct
     4201 gattcattca tgtaatgaat atatccatga tcatcataaa taataataat atctttggca
     4261 ttttcagcaa caaggcggta gcgttcttcg cttgccatga gagcatcctg agcatttttt
     4321 atctgggtta tatcattgaa gaaaagaagc actgcaggtt tctggtacca gatagtaagc
     4381 acagaattta catgaaacca gcgctcttgg ctgtgagtat caataatttt aatggggaac
     4441 agtgtgtatt tttctttacc ttcaattctg tatttatatt cattgcggac aatgtcttta
     4501 aaatcaggat gaacaagttt gtagaatttt ttatttaaaa gctgctcctc agtatatcct
     4561 gttatttcgc ataatttggg gttaacatag cgtacaagct catcctgtat aatggcaatt
     4621 gcttccttgc tgttttcaac aaggaggcga tagttatgct gagtagccag cagtatttct
     4681 tcagcttcct gtaattcagt aagatcaatg attgaagcta cacttttttt tgttcctggt
     4741 attatggtta ttgtcataaa aacattgtga tacctgccag ttttatcaat aagcctgaac
     4801 tcgtaattat tgggaagcga tttgccggag cggcgcaatt catgattttt ttctaaaatc
     4861 cagcggtctt ctggcgccac aaaaaaaggc catttcattt tgccttctat ctcattgcgt
     4921 gtatatcctg aaatcttttc acattcacca ttaaccagtg atattgtttt atcttcttct
     4981 ataaaaagca ttgctgtgcc ggttgactca aaaattgttt tatactgtaa ctcagttttt
     5041 ttcaggcttt cttcggcttt ttttcggttt ttaatatcct tgaaaaagaa aataacaccg
     5101 tccctgcctt caaagtttat gtaggttgac gatatttcaa tatcaatgtt ttcactgtca
     5161 caggctatga ttttatcttc acttgagatg tagcttttgg ggttttcaag aactagggat
     5221 ataacctttc gaagttcatt gtggctctga ggctgcaaaa aactgtatac acttctacct
     5281 ataacatcat caggcctggt agcttttaac agggaaagag cagttgcatt ggcaaatact
     5341 atggtttcat caacatacac aaagattgct tcaggggaaa gatcaacaat atctttaaag
     5401 ctattatgta cagtatgttg ttttaaacag tcagtgttca tggtagttta gacatgcgcc
     5461 atttggcata tacttgtaat acatattata taagacttta tggtttatgt caagtaaaag
     5521 tttaaatata atatatttta tatatgtata taataaatat gttcatatat cttccaatgg
     5581 taaatgttgg aatgctggaa aagatataag aggggtcaga gcatgaattg ataattggca
     5641 atcgcaggcg ctcaagcgct tgagcttctg tgcagaaaag aacaaattgg gtttgacgtg
     5701 gagaacgcag atagttctgg atagattggc tcggagtaat aattaccagg ttttattacg
     5761 tgtggttatg aaatattgcg gagacacggt gttggaggtc aatacctttc gtgcgaagac
     5821 aaaagccttt gatggcatca aaagtacgaa taaaatgggt caatgtgaag caattgctaa
     5881 aggcattttt ttatttggca cttttgttgt tgccgtatgg acaaaataat gtgcaggaaa
     5941 attcattggt ataaaattgg aaaactcctg aaatgtgggg aattgatttg ttaatgtgaa
     6001 ataatatggt tttaggaaaa ttttttaaaa ttttaatagt tcatataact ctatattttc
     6061 ctctacttat atattcaaaa atagcatttg gggtcatagc acaatcacaa gcaccattgt
     6121 tggttttatt gatacagtga tctgaaggtt gaagatatct tatatcagca ttaggataat
     6181 atctttggaa tatagaaatg atttggttcg ttgttaatat ttgaccttta taattagcca
     6241 tcaattggtg taattgacta tgatatttta caaaagcagg catattctaa atcctcctga
     6301 aaaaggtaat ggaataaaaa atattattaa tctacataac aacaagtgga ttacagtcaa
     6361 gaataatttt tattataata aaatcattct atgtcggtaa ctgattaaca ttatattaac
     6421 taaaatatta tcaagatagg gtttaaaaac attttttaga gtaattccca tttgatggtg
     6481 ctattagaaa ctacctgaat cattaacttt ttgataatat atatcagtag cgtttgctgg
     6541 atattcacga taatctaacc atagaggtag tgcaccataa ggagaaccac catca
//
LOCUS       DAORYL010000117         6589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_8136_length_6589_cov_15.595194, whole genome shotgun sequence.
ACCESSION   DAORYL010000117 DAORYL010000000
VERSION     DAORYL010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6589
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8136_length_6589_cov_15.595194"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="PLV81_14165"
     CDS             <1..1235
                     /locus_tag="PLV81_14165"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOM11283.1"
                     /translation="PNRTCMKQKIGIIGAGASGLIAAYFASHNKHTEVHIFEKQNKIG
                     RKVAATGNGRCNLTNMNMGSEHYHSGDIHFVTYVLQHFSLQDTLNFFHSIGIMTTTLE
                     NGKVYPSSLQASTVVKVFEHELSHNNVQIHLSRKIESIQPVHHGFKVITAGKEEHFFH
                     KVILACGSCAYGSLGASKDGYELAQSLGHTIIEPFPAILPLNIVNKQLHTLQGIRWDC
                     TLSVVIGNTTVQQITDEVLFTAYGISGPAALSISRAVNEAVLLNKDVHISCNIFPHYD
                     MHTLRSLFAQLISQPKKSLEFALYGIMNNKMPRVFLSMAGIPYEWPAIESFNDIDTII
                     QAFTRATLYPGKPRPFTEAVVAAGGIDVNEVNPATMESTLHKGLFITGELLDVDGDSG
                     GYNLQFAWSTGAIAGKNV"
     gene            complement(1232..1480)
                     /locus_tag="PLV81_14170"
     CDS             complement(1232..1480)
                     /locus_tag="PLV81_14170"
                     /inference="COORDINATES: protein motif:HMM:NF016262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HOM11284.1"
                     /translation="MNNENDFPQEITFKVICKNFPYIRDVLFNTCGEHAQAITITEKE
                     SRRNTFISYTITATFSTPEHIDRLCNAISHIEGFITMF"
     gene            complement(1534..2349)
                     /locus_tag="PLV81_14175"
     CDS             complement(1534..2349)
                     /locus_tag="PLV81_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11285.1"
                     /translation="MGMHLSADVYDIFEDVFKGKEKAKKVMSALEEVIVTTVHDSWYK
                     TKEELKMEVFSHYATKQDLEELRKELSGKFDVLYEKTEKDKAELLGIINQNKEELLGI
                     MKQDKAELLGIINQSKEELLGIMKQDKAELLGIINQNKEELLGMMKQDKAELTGKIDA
                     LYEKTEKDKAELIGMMKQDKAELTGKIDALYEKTEKDKAELIGMMKQDKAELTGKIDA
                     LYQKTEKDKAELTLRIERLDKKFSIYFAVLLFAIIFLNQNALEFIAKMIGIIR"
     gene            complement(2576..3688)
                     /locus_tag="PLV81_14180"
     CDS             complement(2576..3688)
                     /locus_tag="PLV81_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOM11286.1"
                     /translation="MNDITWIPFDIATQYMIDVFSSIGVPEEDAAICADVLITADKFG
                     IDSHGVNRMKPIYYDRIKIGIQKPVTHFEIIKEGPTTAVVDGHDGMGQVIAYRSMQMA
                     IAKAKKYGLGMVAVRNSTHYGIAGYYAKMAADNNMIGITGTNARPSVAPTFGVENMLG
                     TNPLTFGIPTDEEFPFLLDCATSLSQRGKVEVYARLNKPLPPGWVIDHEGNTCTDPQE
                     VLDGLIKGTMALTPLGGIGEEGAGYKGYGYCTVVEILSAALQAGSYLKMLSGFDTKGN
                     KVPYHLGHFFIAIDIEAFTDINSFKEITGDILRQLRNSRKMPGQERIYTAGEKEYLVY
                     LERKDKGVPVNREVMKELITMRDEQGITGYEMLKLV"
     gene            4133..4897
                     /locus_tag="PLV81_14185"
     CDS             4133..4897
                     /locus_tag="PLV81_14185"
                     /inference="COORDINATES: protein motif:HMM:NF027439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOM11287.1"
                     /translation="MLQKKVEDNVCILTLNDGKMNALTDTVLNDLRNYLKEAAADTSI
                     KGVILTGEGKTFSSGFSLPMFLSFKDVDEVVKFFEVEEEILLELFMFEKPVIAALNGH
                     TVAGGMIFAMACDYRIMKNHPKIKMGMSEIKIGLPLSIAQWNVVRFGLDSDKKFRDIM
                     YFGNMFGPEQALQLGLIDELVDEDKLISRAKELINLWKNNPGNAFTPLKLCIRKETAE
                     RIRKELKEGDMKKELKCFFDENVRKTLEFVQAAMSK"
     gene            4958..>6589
                     /locus_tag="PLV81_14190"
     CDS             4958..>6589
                     /locus_tag="PLV81_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11288.1"
                     /translation="MNIKEAGWKIFSLRSEEVSFTIPLFFLYLFSGIFFATGQVYTES
                     LFLQTYGAAGLSKFFAINGIVLFLSGFIYTYFLELFSLKRAYILLIVLFCCIPGSTLL
                     PSGWAPHDPLYLFIGNYIATFYLDAHFINFSFQFLTLQSSKRIFPFLMAGSKLGGIII
                     TIIISLYSTLFIHYAAYIWLAAGILILIPFFATAKINASTRSHSEHKFLDKLSTLFSS
                     RMFIVSAIAVFVMAIANQHTEYFFARIATHLFPSQETLAVFISRYTFITDFITVAMQL
                     FVTSRIIKKLSIPTANLLYPSSYCLIIMYAVLSPTLLAAVALRFFRKNMSFFIRQPVS
                     NIMLSIYPADRVNQARSIINSIVSPLGMIAGGLLLSLYATLPLHTVFIIALAIGLLFV
                     TITIKQNALYKKAIYFKLQPQLPNTLQISGSDIIEIIKNPNIENKSEFIDTLLQQGMS
                     AELFPMIIQYYDVLSENTKIHLLSIIYEAPEERAVEICKKAVNDENPLVRVYAYRFLS
                     QCAIVTRKEILLQSNPQLAIEPLIQSLLINDNAYSE"
BASE COUNT         1957 a         1401 c         1132 g         2099 t
ORIGIN      
        1 caccaaatag aacgtgcatg aaacaaaaaa taggcatcat tggcgcaggc gcttcaggtc
       61 ttattgcagc gtattttgct tcacacaata agcataccga agtccatata tttgaaaaac
      121 agaataagat tggtcgcaag gtagcagcaa caggcaatgg caggtgtaat ctgaccaata
      181 tgaatatggg gtcagagcat tatcactcag gtgatataca ttttgtcacc tatgttttgc
      241 aacatttttc actgcaggat acactcaact ttttccattc cattggcatc atgacaacca
      301 cgctggaaaa tggtaaggtg tatccttcat cactgcaggc ttctactgtg gtaaaagttt
      361 ttgaacatga actatcgcac aataatgtcc agatacatct ttcacgaaag attgaatcaa
      421 tacaacctgt tcatcacggt tttaaagtaa ttactgcagg taaagaagaa catttcttcc
      481 ataaagttat acttgcctgt ggcagttgcg cctacgggtc actgggtgct tctaaagatg
      541 gctatgaact tgctcagtca ttagggcata ctatcattga accatttccg gcaatactgc
      601 ccttaaacat tgtcaacaag cagcttcaca cgttacaggg aatacgctgg gattgcacat
      661 tgagtgtggt tattggcaac actactgtac agcagataac cgatgaggta ctgttcaccg
      721 cctatggtat ctcagggccg gctgccttga gtatttcccg cgcagtaaat gaagcagtat
      781 tactcaataa agatgtacac atcagctgca atatttttcc acactatgat atgcacacct
      841 tacgttcact gtttgcgcaa cttatttcgc agcccaaaaa atccttagaa tttgcactat
      901 atggcattat gaacaacaaa atgccccgtg tgtttttatc aatggcaggt attccgtatg
      961 aatggcctgc aattgaatca tttaatgaca ttgatacaat cattcaggca tttacccgtg
     1021 caacattgta tccaggtaag ccacgccctt ttactgaggc agtggtagca gcaggtggga
     1081 ttgatgtaaa tgaggttaat cctgcaacga tggaatcaac attgcacaaa ggacttttca
     1141 ttactggaga actattagat gtggatggtg acagcggtgg atataatctt cagtttgcat
     1201 ggagcaccgg agctatcgcc gggaaaaatg tttaaaacat cgtgataaac ccttcaatat
     1261 gtgaaatagc attgcacaat ctgtctatat gttcaggcgt gctgaatgtt gccgtgattg
     1321 tatatgaaat aaacgtatta cgcctgcttt ctttttcggt tatagttatg gcttgagcat
     1381 gttctccaca ggtgttgaaa aggacatccc gtatgtacgg aaaatttttg catataactt
     1441 taaaagttat ttcctgaggg aaatcatttt cattattcat ggagtattcc ttgtgtatat
     1501 ttgcgtttat atattaaaaa ttacaggtta ttttcatcgt attattccta tcattttagc
     1561 aatgaattct aatgcattct gattcaggaa tataatggca aataataaca ccgcaaaata
     1621 tatactgaac tttttatcaa gcctttcaat ccttaatgta agctcagctt tgtctttttc
     1681 tgtcttttga tataatgcat caattttccc tgtaagctcc gctttatcct gtttcatcat
     1741 acctatcagt tcagctttgt ctttctctgt cttttcatat aatgcatcaa ttttccctgt
     1801 aagctccgct ttatcctgtt tcatcatacc tatcagttca gctttgtctt tctctgtctt
     1861 ttcatataat gcatcaattt tccctgtaag ctcagctttg tcctgcttca tcatgcccaa
     1921 aagctcttct ttattctggt taatgattcc caaaagctca gctttgtcct gtttcattat
     1981 ccccaaaagt tcttctttac tctggttaat gattcccaaa agctcagctt tgtcctgttt
     2041 cattatcccc aaaagctctt ctttgttctg attgataatg cctaaaagct ctgctttatc
     2101 tttttcagtc ttttcatata acacatcaaa ttttcctgaa agctctttgc gaagctcttc
     2161 cagatcctgc ttcgtggcat agtgactgaa cacctccatt ttgagctctt cttttgtttt
     2221 ataccagctg tcatgtactg tagtcactat cacctcttcc agtgcactca ttactttttt
     2281 tgccttttct ttgcccttaa aaacatcttc aaaaatgtca tagacgtcag ccgaaagatg
     2341 cattcccatt ttcaatctcc ttccataaaa attgtaacac gttcatattg aaaagtcaat
     2401 catatattga acgttccacc acacttttat atataaaacg ctttgagata aaaaaattta
     2461 aaaaaatttt ttattttttt taatgaagta tttttcttca aaatattata ttcacgtgca
     2521 cattactcca taattgtatt cacaaacaat attccactta ctgtcgctac agcaatcata
     2581 ccaatttcag catttcatat cccgtaatac cctgctcatc gcgcattgta ataagctctt
     2641 tcattacttc cctgttgacc ggcacacctt tatctttgcg ctccaaatat acaagatatt
     2701 ccttttcacc ggcagtgtaa attctttcct gacctggcat ctttcgtgaa ttacgtagct
     2761 ggcgcaatat atcccccgtg atttctttaa acgaattgat atccgtaaaa gcttcaatat
     2821 caatggctat gaaaaaatga cccagatgat acggcacctt attccctttg gtatcaaatc
     2881 cagaaagcat ctttaaatag ctgcctgcct gcaatgcagc cgataatatt tcaaccaccg
     2941 tacagtagcc atatccttta taccctgcac cctcctcacc aatgcctcca agtggggtca
     3001 gagccatagt tcctttgata agtccatcca gcacctcttg cgggtcagta caggtattcc
     3061 cctcatggtc aataacccaa ccgggtggaa gcggcttatt aagccgtgca tatacttcga
     3121 ccttgccccg ctgacttaaa ctggtagcac aatcaagcaa aaacggaaat tcctcgtcag
     3181 tgggtatgcc aaaggtcaaa ggattggtac ccagcatatt ttccacacca aaggttggcg
     3241 caaccgaagg ccgtgcatta gtaccggtta tccctatcat attgttgtca gcagccatct
     3301 ttgcatagta gcctgcaatg ccataatgag tggaattgcg cacagccacc atccccaggc
     3361 catacttttt agccttggcg atagccatct gcattgatcg gtaggctata acctgtccca
     3421 tgccatcatg cccatcaacg actgccgtcg tgggcccttc ctttatgatt tcaaagtgag
     3481 taactggctt ctgaatgcct atcttaatgc gatcataata tattggcttc atacggttaa
     3541 caccatgcga atcaatgcca aacttatcgg ccgtaatcag cacatctgca cagattgcag
     3601 catcttcttc tggtacacca atgctggaaa atacatcaat catatattgt gtggcaatat
     3661 caaagggtat ccaggttata tcgttcatgt atttctcctg tgaaaataga tttaaatctt
     3721 taaccttacc cccagcccct ctccttcgca aggatatggg ttaaaggtaa gatgtgtaaa
     3781 tattttcatt ttgtttaaaa atccacagta atagacaaaa acattactta caaaccatgc
     3841 agcaaaataa aacatcccag aatcaatgag tttatacctt atgtggtaat agtcaaccca
     3901 ataaaacaat ggttaaaaaa tacaaaatac attattttag aagattattt tatcaataaa
     3961 agcaaccatt tctctcaaat aataccataa ggagcaacaa aattaacaca tgtaggctgc
     4021 ataaaaaggt caagagtatt tttccaaaaa tttcttcttt acaactttta cgcctgtaca
     4081 atattgtgta catatatagt taaattttta ttacacataa tgggggcatg gtatgttgca
     4141 aaaaaaagta gaagataatg tttgcattct gaccttgaat gatggcaaaa tgaatgcact
     4201 caccgacacg gtgctgaatg accttcgcaa ttatcttaaa gaagcagcag ctgatacttc
     4261 aattaaaggt gttattttaa ccggggaagg caaaacgttt tcatccggat tcagcctgcc
     4321 tatgtttttg agttttaagg atgtggatga agtagttaaa ttttttgaag ttgaagaaga
     4381 aattttactg gaactgttta tgtttgaaaa gccagtcatt gcagcattaa atggtcatac
     4441 cgttgccggc ggtatgattt ttgcaatggc ctgtgattac cgaatcatga aaaaccatcc
     4501 aaaaatcaag atgggtatga gcgaaattaa aattggtcta cctctttcta ttgcgcaatg
     4561 gaatgtggtt cgatttggcc ttgatagtga caaaaagttc agagacatca tgtattttgg
     4621 caatatgttt ggccccgaac aggcgttgca attaggcctc attgatgaac ttgttgatga
     4681 ggataagctg ataagcaggg caaaagaact tataaatctc tggaagaata atccgggcaa
     4741 tgcctttact cctctgaaac tatgtatacg aaaagagact gcggaaagga ttcgaaaaga
     4801 gctaaaagaa ggcgatatga aaaaagaatt gaagtgtttc tttgatgaaa atgtccgcaa
     4861 aaccttagaa tttgtacagg cagcaatgag caagtaattt ttatgtccga tagttccgat
     4921 aatacaagca aaggagctat gggacttcat ctttattatg aatataaaag aagcgggatg
     4981 gaaaatattt tcactgcgca gtgaggaagt atcattcacc atcccgcttt tctttctgta
     5041 tctgttctca ggaatattct tcgcaactgg tcaggtatat accgaatcac tgtttttaca
     5101 aacatacggg gcagccgggc tatccaaatt ttttgcaatt aatggcattg tattattcct
     5161 ttctggattt atctacacct atttccttga attattttct ttaaagcggg catatattct
     5221 gctcattgtg ttattctgct gtatacctgg cagcacacta cttccttcag gctgggcacc
     5281 acatgaccct ctttatcttt ttattggcaa ttatattgca acattttatt tagatgctca
     5341 tttcattaat ttttcatttc agttccttac cctgcaaagt tccaaacgga tattcccgtt
     5401 tttaatggct ggatccaaat taggcggaat aattattacc attataattt ccctgtacag
     5461 tacactcttt attcactatg cagcgtatat ctggcttgca gccggtattc tgatacttat
     5521 cccatttttt gcaactgcaa aaatcaatgc gagcacacgt agccattcgg aacacaaatt
     5581 tttggacaaa ctatctactc tgttttcaag ccgcatgttt attgtaagcg ctattgcagt
     5641 ttttgttatg gcaattgcca atcaacacac ggagtatttt ttcgcacgca tcgccacaca
     5701 tctttttcct tcacaggaaa cactggctgt ctttataagc aggtatacat ttataaccga
     5761 tttcataaca gtagccatgc aactttttgt tacatcacgc atcattaaaa aattaagtat
     5821 tccaactgcc aacctgctct atccctcatc atactgctta attatcatgt atgcagtgtt
     5881 atcacccaca ttgcttgctg ctgtagcatt gcgttttttc cgcaaaaata tgagcttctt
     5941 tattcgtcag cctgtcagca atataatgtt gtctatctat cctgctgaca gagtaaacca
     6001 ggcgcggtcc atcatcaaca gcattgtaag cccactgggc atgatagcag gcgggctatt
     6061 actttctcta tatgcaacac ttccattaca tacggttttc atcatagctc ttgcaattgg
     6121 attattattt gtgaccataa ccataaaaca gaatgccctg tataaaaaag caatctattt
     6181 caaattacag ccacagctac caaacacact acaaatatct ggcagtgata tcattgagat
     6241 tatcaaaaac ccaaacatag aaaataaatc tgaatttatt gatacgctgc tacagcaggg
     6301 tatgtcagca gaactgtttc ctatgataat ccagtattat gatgtgctat ccgaaaacac
     6361 caaaatacac ctgctttcaa ttatatatga agcaccagaa gaacgtgctg ttgaaatatg
     6421 taaaaaagca gtaaatgatg aaaacccgct ggtacgagta tatgcctatc gttttctatc
     6481 acaatgtgcg atagttaccc gtaaggaaat tttgctacaa tctaacccac aacttgcaat
     6541 tgaacctttg attcaatctt tgctgataaa tgacaatgca tacagtgaa
//
LOCUS       DAORYL010000118         6262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_8623_length_6262_cov_13.288706, whole genome shotgun sequence.
ACCESSION   DAORYL010000118 DAORYL010000000
VERSION     DAORYL010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8623_length_6262_cov_13.288706"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PLV81_14195"
     CDS             complement(<1..614)
                     /locus_tag="PLV81_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11289.1"
                     /translation="MYIIRENFYKLLTNLIITIFFIFACDEPSHGHGKNELWYYPDCY
                     TIQTAGYIGFIAMGIGFLSFNNTWESSLLYGFSPADVTGKEIHTVSWKNIVYPFTLNF
                     LDDNVLLPFYFGVTMLFVMDKNVYWERSTNCPDRYYNPTGRHSAVSVGLQIQGKSTMI
                     GLIHGFYVEITILDTYLKACLWDDSEYLNFDDVASLAIGYKIIT"
     gene            complement(602..1414)
                     /locus_tag="PLV81_14200"
     CDS             complement(602..1414)
                     /locus_tag="PLV81_14200"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOM11290.1"
                     /translation="MKVFSKATIPCACYILIVTICILFQGCYLGLEYSPYSTDVDYKN
                     LNKKNIDRIKARESESSNYRIALIADTHRFYTETENVIKVLNSRNDLDFVFILGDITD
                     VGLLKEYNFAYDIFSKLYCPFIVIAGNHEFLGYGENIYKKMFGPLNFNFTFRGTEFIC
                     FNDSNWESGEPDWWWLEQTAALSYEQHKIVLAHIDCSITSERFNQEQVDRFNDIVKSY
                     FDMAFHAHDHVNSYKLIEDIPRYHVGSSHHRHYVILEYDYNTDNFTVELCTL"
     gene            complement(1457..1744)
                     /locus_tag="PLV81_14205"
     CDS             complement(1457..1744)
                     /locus_tag="PLV81_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11291.1"
                     /translation="MVMLFIVYSNAVDDEVVEIVKKYASGYTKFVDVQGEGNKEPQLG
                     THIWPGLNNCMMIALEDNKSKDKIAQELQKLKVKFKGVGISVFVVQLKEIL"
     gene            complement(1775..4888)
                     /locus_tag="PLV81_14210"
     CDS             complement(1775..4888)
                     /locus_tag="PLV81_14210"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOM11292.1"
                     /translation="MKLTDISVQRPITITMLYIGIIVVAIVSYVKIPLDLFPDIEFPV
                     AVVFTEYPNVGPKEIESTVTRPLEEALSSVNNIDTITSTSKEGLSMIVAKFTWGTDMG
                     LAVSDMRERIDIRKRFLPDDVENPVVLKFDTSMMPVVMLSVTGPKDLAYVKKYAEDNL
                     KNLFEQTDGVAAAYVSGGQSYEVKVELIKNRIDAYKLTIDQIIQLLAAENLNIAGGDV
                     KTPYMKYTLRTQGEFKSLDDIRNVVVTVKNGTPIYVRDIARVYLAPADVKEIVRLNGE
                     NGVILRVQKQSDKNTVIVARNILKKAEEVRKTLPKGMDIQPFFNAAEFIELSISNMIK
                     NAVEGALIAILVVLVILRNMRAALIMGLSIPVSIITSFVLMYFFDLSLNMMSMGGLAI
                     GVGMLIDNSIVVLENIFRYREKGARPNEASKLGADEMGMAITASTLTNLVVFVPFLFS
                     QGLAVQLFKDMALTITFSMLSSLVVALSLVPMLAAKFIPRIEHTYTGKLSFLQPLFDR
                     SEQYFIRLERFYGKVINWALDHRRRVIRYVAISFVIGLIMIPIAGMEFMPEYDDAQLT
                     IVAKLPVGTNLETTESVMKMIEKRALDVLKKEEYVVVLVRAGYGEGFTAAFGETTDYT
                     GKIEMRLVQLSKRKRSKNEIRQALREAFKGIPGVTFNFSLQGKGGRILGMGGATISIE
                     VYGYDFEKSSQYVEDIRNAIKNIPGLKDIDVSREEGLPEKVIVVNREKASKMGVNAYQ
                     IANLIKNNVAGYPATRYRFEGDEYDINVRLREADRETIEHIKSIEINTPIGKRVPVGN
                     LIEIVTKSGPSTIERKMQERVVYLNCKAEGRALSAVVSDINKAISKLPKPANFDVVIA
                     GAYKDMQDTFQDLLLVMLVAMVLTYVILAAQFESLLRPFIVMFSVPTVIFGVSLFLFL
                     TGTTFNVFTFLGVIMLVGIVVNNAIVLVDYTDILRKRGYGIREALVEAGTKRLRPIMM
                     TTLTTILSLIPMASGLGEGSELSSPLARAVMGGMSTAFIFTLVFIPVMYMIFETIRER
                     YEARRKY"
     gene            complement(4910..5833)
                     /locus_tag="PLV81_14215"
     CDS             complement(4910..5833)
                     /locus_tag="PLV81_14215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOM11293.1"
                     /translation="MKKGTKIIIVLILLIIAIIGTYRLVVFIGNKIKPKQTAQEERIL
                     PVKAAKAVRMDITKTLNLSGDIVGKEVVNVFSQVPGKVMEILVKEGQFVKKGQVLFRV
                     DRDIVGMEYMPAVVESPLSGYVGTVMVDRGMSISPQTPLAQVVTMNQVEAVAQLIEEQ
                     INMVQIGMKAKVRVDAYPNVYFYGTVYRKNAVLDAASRTQEVRILIDNPELKLRHGMF
                     ADIQIQMGSAKNAVVIPVDSILSDSSGNYYVYKVVDNKAFKQAIKPGFSLKNVIEVVS
                     GINEGDVVVTLGKENVGHGDKLMVYMEEIKQ"
     gene            complement(5867..>6262)
                     /locus_tag="PLV81_14220"
     CDS             complement(5867..>6262)
                     /locus_tag="PLV81_14220"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOM11294.1"
                     /translation="TFGATYTWGAWSPIDSSHAKAKQLESQANQLDLQLQDFVKSVRL
                     DIQRGFLKLKSASNSLYSQQGNVETAEESLKTATVQFRNGIIDNSKFLEANVSLIQAK
                     TLYIQALYDYKVAQAELNKAIGVDYFKIQ"
BASE COUNT         1930 a         1394 c          989 g         1949 t
ORIGIN      
        1 tttgttatta ttttatatcc tatagcaagg ctagcaacat catcaaaatt tagatattca
       61 ctatcatccc agaggcatgc ttttaaatac gtatcaagta tggttatttc aacataaaaa
      121 ccatgtatta aacctatcat tgtgcttttc ccctgtatct gtaatccaac acttacggca
      181 ctatgccttc ccgtggggtt ataatacctg tcagggcagt ttgtggatct ttcccaataa
      241 acatttttat ccatcacaaa aagcatcgtg acaccaaaat aaaatggcaa tagtacatta
      301 tcatctaaaa aatttaaagt aaatgggtat acgatatttt tccaggaaac tgtatgtatc
      361 tccttacctg taacatctgc aggactaaaa ccatacaata gagagctttc ccaggtattg
      421 ttgaaagaaa gaaaaccaat gcccatagct ataaatccaa tatacccggc agtttgtatg
      481 gtataacaat caggataata ccataattca tttttgccat gcccatgtga aggttcatca
      541 caagcaaaaa taaaaaatat ggttataatt aaattagtaa gtagcttata gaaattttct
      601 cttataatgt acataattca acagtaaaat tatctgtatt gtaatcatat tctaatatga
      661 cataatgtct atgatgtgag ctacccacat gatagcgggg aatatcttca atcagtttat
      721 aactgttgac atggtcatga gcatgaaagg ccatatcaaa ataactttta acaatgtcat
      781 tgaatctatc tacctgttcc tgattgaatc tctcagatgt aattgaacaa tcaatatgag
      841 ccaatactat tttatgttgc tcataactta aagcagcagt ctgttccaac caccaccaat
      901 caggttcacc gctttcccaa ttactatcat taaagcaaat aaattcagtt ccacgaaacg
      961 taaaattaaa atttaatggc ccaaacatct ttttataaat attttcgcca tatccaagaa
     1021 attcatggtt gccagcaatg acaataaaag ggcaatacaa ttttgaaaat atgtcatagg
     1081 caaaattata ttctttgagt aaaccaacat cagtaatatc acctaaaatg aatacaaaat
     1141 ccagatcatt ccggctattt aatactttaa taacattttc agtttctgtg tagaaacgat
     1201 gggtatcggc tataagcgca attctataat tactggattc tgattcacgc gctttaatac
     1261 ggtcaatatt tttcttattg agatttttat aatctacatc agttgaatac ggggaatatt
     1321 ccagcccaag ataacacccc tgaaatagta tacatatagt tactatcagg atataacaag
     1381 cacagggtat agtagcttta ctgaaaactt tcactgcttt aaacctctgt tttgctgtgt
     1441 cagtaatcct tattttctat agaatctctt tcaactgcac cacaaagaca ctgatgccaa
     1501 cacctttaaa ttttactttt agtttttgta gctcctgggc aattttatcc ttagatttat
     1561 tgtcttcaag agctatcatc atacagttat tgagccctgg ccatatatgg gtacccaatt
     1621 gcggttcttt atttccttca ccctgcacat ctacaaattt tgtatatcct gaagcgtatt
     1681 tttttactat ctcaacaact tcatcatcaa cagcgttgct gtaaactata aatagcatta
     1741 ccatactaat tatctcctct gcaaataaat ttaattaata tttccgtcgt gcttcatacc
     1801 gttcacgtat ggtttcaaat atcatataca ttaccggtat aaacaccagg gtaaagataa
     1861 acgctgtact cattccaccc ataacagcac gcgctaatgg agatgaaagc tctgatcctt
     1921 cgcccaggcc tgaagccata ggtataagcg atagtatcgt agttagcgta gtcatcataa
     1981 ttggtcgtaa tcgttttgta cctgcttcaa ccagtgcctc tcgtatgcca tacccgcgct
     2041 ttcgcaggat atcagtataa tccacaagaa ctatcgcatt gttaacaaca ataccaacta
     2101 gcatgatgac acctaaaaat gtgaacacat tgaatgttgt accggttaag aacaaaaata
     2161 gtgacacacc aaaaataacg gtaggcacac tgaacataac aataaatggc ctaagtaacg
     2221 attcaaactg agctgccaaa ataacatagg tcagcaccat ggcaaccagc attaccagca
     2281 ataaatcctg aaatgtgtcc tgcatatcct tatatgcacc agctatcacc acatcaaaat
     2341 tagcaggttt gggcaattta ctgattgctt tgttaatatc cgaaacgaca gcactcaaag
     2401 ctctaccttc agctttacag tttaaatata caacacgctc ttgcattttc cgctcaatgg
     2461 ttgacggccc tgatttagtc acaatttcaa tcaggtttcc tactggcact cgcttgccaa
     2521 tgggagtgtt tatctcaatt gattttatat gctcaatagt ttctctgtca gcctctcgta
     2581 aacgaacatt aatatcatac tcatcgccct caaagcgata tcgggttgcc ggataccctg
     2641 caacattatt ttttataaga tttgcaatct gataggcatt gacacccatc ttggaagctt
     2701 tttcacggtt aacaactata accttttcgg gcaatccttc ttcccgtgaa acatcaatat
     2761 cttttaaacc cggaatattt ttaatagcat tgcggatatc ttcaacatac tgtgatgatt
     2821 tctcaaaatc atatccatat acttcaattg aaattgtagc tccacccatt cctaagattc
     2881 taccaccctt accctgaagg gaaaagttaa acgtaacacc cggaattccc ttaaatgctt
     2941 cacgtaaagc ctgccgtatt tcattttttg accgtttgcg tttacttagt tgaaccagcc
     3001 tcatttcaat cttgccagta taatctgttg tttcaccaaa cgctgcagta aacccttccc
     3061 cataaccggc tctgaccaaa acaacgacat attcttcttt ttttaacaca tctaacgctc
     3121 gtttttcaat catcttcata accgattcag ttgtttccaa attagttcca acgggcaatt
     3181 ttgcaacaat agtgagttga gcatcatcat attctggcat aaattccatt ccggcaatag
     3241 ggatcatgat aagcccaatc acgaatgaaa ttgcaacgta tcggataact cgccttctgt
     3301 ggtcaagtgc ccagttgata acttttccat aaaaccgttc caatcgtata aagtattgtt
     3361 cacttctatc aaataatggc tgcaaaaatg aaagtttccc ggtataggta tgttcaatgc
     3421 ggggaataaa ctttgctgca agcattggca caagtgatag tgccacaacc agtgaactta
     3481 acattgaaaa cgttatggta agcgccatgt ctttaaatag ctgtacagca agcccctggc
     3541 tgaataaaaa tggtacaaag acaaccaggt tggtaagggt tgacgctgta atggccatac
     3601 ccatttcatc ggcacctaat tttgatgctt catttggccg tgcacccttc tccctatatc
     3661 tgaaaatatt ttccagtacc acaatggagt tatcaataag cataccaaca ccaatggcaa
     3721 gcccacccat tgacatcatg ttgagtgaca ggtcaaagaa gtacatcaga acaaatgaag
     3781 taataataga aacaggtatc gacaatccca taatgagtgc agcccgcata tttcgcagta
     3841 taacaaggac aacaagtata gctattaaag ccccttcaac agcatttttt atcatattgg
     3901 atattgatag ctctataaat tcagcagcat tgaagaatgg ctgtatatcc atccccttgg
     3961 gcagggtttt gcgtacttct tcagcttttt ttaaaatatt gcgggcaaca ataacggtat
     4021 ttttatccga ttgtttctga acacgcaaga taacaccatt ttcaccatta aggcgtacta
     4081 tctctttaac atcagcaggg gctaaatata ctctggctat atctcgcaca taaattgggg
     4141 tgccattttt tactgtaaca accacattgc gaatatcatc aagagacttg aattcgccct
     4201 gagttcgcaa ggtatatttc atgtatgggg tcttgacatc accgccagca atatttaagt
     4261 tttctgccgc cagaagctgt ataatctggt cgatagtcaa tttataagca tcaatcctgt
     4321 ttttaataag ctcaacctta acttcataac tctgcccacc actgacgtat gccgctgcta
     4381 caccatcggt ctgctcaaat aaatttttta aattgtcctc ggcatatttt tttacatacg
     4441 ctaaatcttt aggtccggta actgaaagca tgacaacagg catcatggaa gtatcaaatt
     4501 taagtaccac aggattttca acatcatcag gtaaaaagcg tttgcggata tcaatacgct
     4561 cgcgcatatc acttactgcc agccccatgt cagtacccca ggtaaacttg gcaacaatca
     4621 ttgacaggcc ttcttttgat gttgacgtta tggtatcaat attattaacc gatgacaatg
     4681 cctcttccag tggtcgggtt acggtacttt ctatctcttt aggaccaaca ttgggatact
     4741 cggtaaaaac aacagcaacg gggaattcaa tatcaggaaa taaatctaaa gggattttta
     4801 cataactcac tattgccact acaattatac caatgtataa cattgtgatt gttattggcc
     4861 tttgaacgga tatatcagtt aatttcatag taactatctc cctgcactat tactgtttaa
     4921 tttcttccat ataaaccata agcttatcgc catgccccac attttccttg cccagtgtaa
     4981 caaccacatc accttcatta ataccggata ctacttcgat aacatttttt aaggaaaatc
     5041 cgggctttat tgcttgttta aatgccttgt tatcaactac cttgtataca taataattac
     5101 cggagctatc gcttaaaata ctatctaccg ggatcaccac cgcgttcttt gctgaaccca
     5161 tctgtatctg aatgtctgca aacatgccat ggcgtagctt aagctctgga ttatctatga
     5221 gtatacgaac ttcctgcgtt cgacttgctg catcaagtac cgcattttta cgatacactg
     5281 tgccatagaa atatacattg ggatatgcat caacccgtac ctttgccttc atgccaatct
     5341 gtaccatgtt gatctgctcc tcaatgagct gagcaaccgc ctcaacctga ttcatggtaa
     5401 caacctgagc aagtggagtc tgcgggctta tggacattcc cctatcaacc atcacggtgc
     5461 ctacatatcc tgagagtggc gattcaacaa ctgcgggcat gtattccata ccaacgatgt
     5521 cacgatcaac cctaaagagt acctggcctt tttttacaaa ctggccttcc tttacaagga
     5581 tttccataac cttgcccggc acctgtgaaa agacattcac aacctcttta cccacaatat
     5641 cgccactgag attaagggtt tttgttatat ccatgcgcac tgcctttgct gcttttacgg
     5701 gtaaaatacg ttcctcctgg gcagtctgtt taggcttaat tttattacca ataaatacta
     5761 ccaatctgta cgttcctatg attgctataa tgagaagaat taagactatt atgattttgg
     5821 ttcctttctt cattatttat cctccataaa atcgttgaac tatcttttat tgaatcttaa
     5881 aataatccac accaattgct ttgttcaact cagcctgtgc aaccttataa tcatacagcg
     5941 cctgtatata caaggtctta gcctgaataa gcgatacatt tgcctctaaa aattttgagt
     6001 tatcaataat tccatttcta aactgcactg ttgccgtttt caatgactcc tctgcagttt
     6061 caacatttcc ctgctgtgaa tacaaagaat tggaagccga tttcagcttt aaaaatccgc
     6121 gctgaatatc aagtcgcaca cttttcacaa aatcctgtaa ctgcaaatca agctggtttg
     6181 cctggctttc cagttgtttt gcctttgcat gtgatgaatc aattggtgac catgcccccc
     6241 acgtataggt agcaccaaag gt
//
LOCUS       DAORYL010000119         6199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_8734_length_6199_cov_10.660482, whole genome shotgun sequence.
ACCESSION   DAORYL010000119 DAORYL010000000
VERSION     DAORYL010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8734_length_6199_cov_10.660482"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..555
                     /gene="trxA"
                     /locus_tag="PLV81_14225"
     CDS             244..555
                     /gene="trxA"
                     /locus_tag="PLV81_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOM11295.1"
                     /translation="MANIQEVTDATFDSAVLQSQGKVLVDFWAPWCGPCRLQTPILEK
                     LASNGINAKIVKCNTDVNPAIARKFGISAIPTLILFENGKEIDRMIGVQPEEVLKRRL
                     Q"
     gene            complement(596..1357)
                     /locus_tag="PLV81_14230"
     CDS             complement(596..1357)
                     /locus_tag="PLV81_14230"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOM11296.1"
                     /translation="MHLHNVSLWRNSVTILKNINLTIKYGEQWAVLGPNGSGKSFLMN
                     IIATLVFPSSGEVVIAGKKLGEVNVWDVRKHIGIVSDYLQYSYPAMTKTVEVVASGFY
                     SSLGLYQELSPTILQKAESILQTLGLEHYAQTPFGKLSYGEQKRVLIGRAIVYDPDIL
                     ILDEPCNGLDIRAREEFLHKLTDLVAIKKHIIYVTHHVDEIMPWINRVMLLADGQCMY
                     AGDRSVLEDEALLSRVMGYPLGIFHHNSRTYWYIK"
     gene            complement(1419..2402)
                     /locus_tag="PLV81_14235"
     CDS             complement(1419..2402)
                     /locus_tag="PLV81_14235"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOM11297.1"
                     /translation="MKKLILLICLSLIFVLSCTKEPKQDVLKIHSFFGDVTVQLQDTT
                     RVPEIGQLLSLNDVVITGKGAIVDLVYRNAGIIRINENTTVKIESLMKGNNDDVVLTV
                     DTGKTFATLGKLKKGDNFALKSKTVIAAVRGTSFRIVASEKGSSVDVVTGKVMVKPVS
                     NNTVVEDVEVVVEENQTVALDTKAVETIVQKIEEKKAETTEKDTQQKIVEEIQESIKP
                     IETPKETIQEIKQEVKDIPVVAVVHEEVKQEISRVVEEDKEAKKREEEEKLKKEKAEK
                     ELQLKLEKERKAKMMAQKLEQQRLEKEKAEREKAAKEQKIKEDRVKNIPTL"
     gene            2793..3269
                     /locus_tag="PLV81_14240"
     CDS             2793..3269
                     /locus_tag="PLV81_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11298.1"
                     /translation="MEIETTTCLSLEHYQLIQLLSEQYAMPMRSFISALIGFAAEIDL
                     MPPTTFTCLTYRKRGTTWKRLHLMLFADEYEFFMDIRKVWKMSLARVIEFCLDNILDE
                     FLRFLDSKIVEEDYYTDSYRYRNYCFEFCREENIQTGKFYWGIHPEIIQKAKNATP"
     gene            3423..4733
                     /locus_tag="PLV81_14245"
     CDS             3423..4733
                     /locus_tag="PLV81_14245"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOM11299.1"
                     /translation="MNEQISINDTTIDSLGECTYPSPLKISKFIDDSQRIAIDIEAHL
                     LEQSFHTTGTIASFENAGPRKKIFFNPETTRAAIVTCGGLCPGINNVIQGIVRMLNFQ
                     YGVKTIYGIRYGFEGLVKRYGHSFIELTPAFVHDLHEKGGTVLGSSRGHQDEKEIVAT
                     LTEHGINILFIIGGDGSLRGAYDIYQEVKRQKLPIAIVGIPKTIDNDIWFVDKTFGFS
                     TAVSTAAQAIYAAHSEAIGAKNGIGIVRVMGRDAGYIAAYVTLASNEVNYCLVPEVHF
                     DLEGPYGFLTHLEKRLYAASHAVIVVSEGAGQEYFTGPKETDASGNIKYGDIGLLLKD
                     KITEYCKKRQIPVTIKYIDTSYLVRSVPASSDDAVYCIMLAQNAVHGAMAGKTGFVVG
                     SWHSYYTYIPLSLVTKKRKRIDPNGYLWSMVKQSTCQPDFSPPQ"
     gene            4857..5834
                     /locus_tag="PLV81_14250"
     CDS             4857..5834
                     /locus_tag="PLV81_14250"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOM11300.1"
                     /translation="MDQLYRIKQLYANIGKLIVSSLELDHILEGIMEEVHTYFAPQNW
                     SLLRYDPNSEELYFVISHGIDFDSVKNIRLKKGEGVAGYVVETKKPVFVPDTSIDTRF
                     SDKIDRITGFSTKSVMAVPLIFRDTIYGVIELINRAQGGIFSSDDYLILQTIADFSAI
                     AFANSLLYEKASTIAVLDPLTGLFNSKKLTDIINVWSREASHLRREDTTNIIAVLLLD
                     IKGLNDINNIGGHREGDRILKQLSRLLKTSLRENDMIFRTGGDMFLAIINCDNYFDLK
                     SLVERIVQKANNITLPDHPELHITIHHGLAIGTRNRIQELIEEAHRQKI"
     gene            complement(5835..>6199)
                     /locus_tag="PLV81_14255"
     CDS             complement(5835..>6199)
                     /locus_tag="PLV81_14255"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOM11301.1"
                     /translation="KDETVIARAHNKTRQYSDPTAHAEILAIKDAAHFLHNERLTGCL
                     LYVTKEPCAMCAGAIVHARIEKLFIGTRDYRFGACGTVLTVCGSSQLNHIPEIMFGIQ
                     EEKCKKLLQDFFGKLRST"
BASE COUNT         1868 a         1262 c         1092 g         1977 t
ORIGIN      
        1 ataaattatc gctttatagt atgctctgac cctttcattg tttctaaacc gataactatc
       61 gcaactcatt ttgtacacaa aacagatcaa gtatagtaat gcctgcataa tgtatacgta
      121 tgctctgacc ccttagccag taactatcgc cacattttta tagaattcat aaaatttttg
      181 ttgccgatat ttaagtattt tattatattc ttaatagata ttttattacg gaggaacata
      241 ctgatggcaa atattcaaga agtaactgat gctacatttg attctgcagt gttacaatcg
      301 cagggtaaag ttcttgttga tttctgggca ccatggtgcg gcccatgcag attacagaca
      361 cccatcctgg aaaaattagc atccaatggc attaatgcaa aaattgtaaa atgcaatact
      421 gacgttaatc cagctattgc acgtaaattt ggcataagcg ccataccaac actgatatta
      481 tttgaaaacg gtaaagaaat tgaccgtatg attggcgtac agcctgaaga agtcctcaaa
      541 cgcaggctcc aataacccac tccacccttt aaaacggctg gtgaaaaccg gccgtttatt
      601 ttatatacca gtacgtccgt gaattatgat ggaaaatccc aagagggtaa cccataaccc
      661 tgcttaacag tgcctcatcc tcaagcacac tgcgatcacc tgcatacatg cactgcccat
      721 ctgcaagaag catcacacga ttaatccacg gcatgatttc atcaacatga tgcgtaacat
      781 agataatgtg tttctttata gcaacaagat ccgttagttt atgaagaaac tcctcacgtg
      841 cgcggatatc aagcccatta catggctcat ctaaaatgag aatatcagga tcatacacaa
      901 tagccctgcc aatcaacacg cgcttttgct cgccatacga aagttttcca aacggcgttt
      961 gcgcatagtg ctcaagaccc agtgtttgca gaatgctttc agctttttgt agtatagtgg
     1021 gcgatagttc ctggtataac cctaaactgc tataaaaacc tgatgctaca acctcaactg
     1081 tttttgtcat tgccggatac gaatattgca gataatcgct tacaatgcca atatgtttgc
     1141 gaacatccca cacattgact tcacccaatt tttttccagc aataacaact tccccacttg
     1201 aagggaatac cagcgtagca ataatattca tcaaaaacga ttttcccgaa ccattggggc
     1261 caagcacagc ccactgttcc ccatatttaa tggtaagatt aatgtttttc aggatggtaa
     1321 cagaatttcg ccatagtgat acattatgta aatgcaaaag ctccatagta tatgaattag
     1381 catataatct atggagcttc taaatattta ctattgtttt aaagcgtagg gatatttttt
     1441 actctgtcct cttttatctt ctgttctttg gctgcttttt cacgctccgc cttttctttc
     1501 tccagccttt gttgttctaa tttttgtgcc atcatctttg ctttacgctc tttttccagt
     1561 ttcagttgca gttctttttc agctttttct ttctttaatt tttcttcttc ctcacgtttt
     1621 ttggcttcct tatcctcttc aaccaccctg ctaatttctt gtttcacttc ttcatggact
     1681 acagcaacga cgggaatatc ttttacttcc tgcttaattt cttgtattgt ttcctttgga
     1741 gtttcaatgg gcttgatgga ttcttgtatt tcttcaacta ttttctgctg tgtatctttt
     1801 tcagttgttt ctgccttttt ttcttctatt ttttggacta tagtttctac tgcttttgta
     1861 tccagtgcaa ctgtctggtt ttcctcaact accacttcaa catcttccac aactgtatta
     1921 ttgcttacag gcttaaccat taccttaccc gtaacaacat caacactgga acctttttcg
     1981 cttgcaacta tcctgaatga tgtcccacga acagcagcta tcaccgtctt tgattttaat
     2041 gcaaaattat cacctttttt aagctttccc aatgtggcaa acgttttacc agtatcaacg
     2101 gtaaggacaa catcatcatt attccctttc atcagagatt caatctttac cgttgtattt
     2161 tcattaattc tgataatacc ggcattacga taaaccagat caacaatggc accttttccg
     2221 gtaataacaa catcattcaa agacagtaac tgcccaattt ccggcacacg ggtagtatcc
     2281 tgtaactgta ctgttacatc accaaaaaac gaatggattt ttaatacatc ctgtttgggt
     2341 tctttggtgc aggataatac aaaaatcagg cttaaacata ttaatagtat taattttttc
     2401 atgataaaac tccgttcaat aaaattaata tatgaatatt actatctatg acatggttac
     2461 atgtaactat cgatttataa aaaattcctc tcataacttt ttttcaaaat cttaaaatac
     2521 atacagcaca tgatttcttt ttataagata agctattcca gtattaaatc aagaattttt
     2581 ctatctcatt attaaaccat actatactac tgcataaaat taaaaaagtg taaagaaaaa
     2641 aataataact cattaaaatt atgtatagta cagtatttag atgagatagt taatagtaat
     2701 tataataatt tttcataaat aatgcttgcg ttttacaata tttttgtata gtatatttaa
     2761 aaaaaataca gagataatcc cggaggaata caatggaaat tgaaaccaca acctgtcttt
     2821 ctttagaaca ttatcagctt attcaattac tatcggaaca atatgctatg cctatgcgat
     2881 cttttatatc agctctgatt ggttttgctg ctgagataga tttgatgccg cctactacat
     2941 ttacgtgtct tacctataga aagcgtggaa caacctggaa acgattgcat ttaatgcttt
     3001 ttgctgatga atacgaattt tttatggata ttcgtaaggt gtggaagatg tcgctggcaa
     3061 gagttattga attttgcctg gacaatattt tagatgagtt tttacgattt ttagatagta
     3121 aaattgtgga agaagattat tataccgata gttatcggta caggaactat tgttttgaat
     3181 tttgcagaga agagaatata caaacaggga aattttactg gggaatacac cccgaaataa
     3241 tacaaaaagc aaaaaatgca actccatgaa ttgaaaattt gattaatata gccataattc
     3301 cagcttatat tacagtatca gatttttttc aattgacttt ataaaaaaca aaaatatcct
     3361 gcatcatcaa aattttttga tatatttact atataaacat ataaactatc agattgactg
     3421 ttatgaatga acaaatatca atcaatgaca caacaattga ttcattgggg gaatgtacat
     3481 atccatcccc gttaaaaata tcaaagttta ttgatgatag tcagcgtatt gccattgaca
     3541 ttgaagcaca cctgttagag caatcgtttc ataccacagg tactatcgcc tcatttgaaa
     3601 atgcagggcc acgtaaaaaa atattcttta atcctgaaac taccagagca gccattgtta
     3661 cctgcggtgg gctgtgcccg ggtataaata acgtcattca gggaattgtg cgaatgctca
     3721 attttcaata cggtgttaaa acaatttatg gcattcggta tggatttgaa ggcctggtta
     3781 agcgctatgg acattcgttt attgagctaa cccctgcatt tgtccatgat ctccatgaaa
     3841 agggcggcac tgtattagga tcttcacgcg gacatcagga tgaaaaggag atagttgcca
     3901 cgctgacaga acatggtatt aatatattgt ttatcattgg cggtgatggc tcattgcgtg
     3961 gcgcgtacga catctatcag gaggttaaaa ggcaaaagct ccctattgcg atagttggca
     4021 ttccaaaaac tatcgataat gatatatggt ttgtagataa aacctttgga ttttccaccg
     4081 cagtgtccac ggctgcacag gctatctatg ctgcgcattc tgaggctatt ggtgcaaaga
     4141 atggcattgg tattgtgcgt gttatggggc gcgatgccgg atacattgca gcatacgtta
     4201 ccttagcgtc aaacgaggtt aactactgcc ttgtccctga agtacatttt gacttagaag
     4261 ggccgtatgg ttttttaacg cacctggaaa agcgcctgta tgctgcatct catgcagtca
     4321 ttgttgtttc tgaaggtgct ggtcaggagt acttcaccgg gccaaaggaa accgatgctt
     4381 ccggaaatat taaatacggg gatattggac tgcttttaaa agataaaatt acggaatact
     4441 gcaaaaaacg gcaaattccg gtaaccataa aatatattga cacaagctat ttagtccgaa
     4501 gtgtccctgc ttcaagcgat gatgcggtat actgcattat gcttgcgcag aatgctgtgc
     4561 acggtgctat ggcaggcaaa accggttttg tggtaggaag ctggcattca tattacacct
     4621 atattccttt gtcattggtt actaaaaaac gaaagcgtat tgaccccaat gggtacctgt
     4681 ggtcaatggt aaaacagagc acctgtcagc ctgatttttc cccaccccaa taacgatcat
     4741 aaaaatacac aaccactgat tttttatttg cattatttac ttatatattt tacttttcac
     4801 acgaaaattg caaataatta gaatacaagc acttttgcga tatgactact aatgatatgg
     4861 atcaactgta tagaataaag cagctatatg ctaatatagg caagcttatc gtttcctcac
     4921 ttgaacttga ccatattctg gaaggcatca tggaggaagt tcacacctat tttgcacccc
     4981 aaaattggag cttgctacgc tatgatccca attctgagga attatacttt gtcatttcac
     5041 atggtattga ctttgacagt gttaaaaata ttcgcctgaa aaaaggtgaa ggggttgcag
     5101 gatacgtagt ggaaactaaa aaacccgtct ttgtacctga caccagcatt gatacccgct
     5161 tcagcgacaa aattgacaga attaccggct tttctacaaa atccgtcatg gctgtcccgc
     5221 taatatttcg cgacactatc tatggagtta ttgaacttat caacagagct caaggtggaa
     5281 tattttcttc cgatgactat ctcatattgc aaaccatagc tgattttagt gctattgcct
     5341 ttgccaattc cctgctttat gaaaaggcat caaccattgc tgtacttgat ccgctcaccg
     5401 gtttgttcaa ctcaaaaaaa cttactgaca taattaatgt atggtccaga gaagcatctc
     5461 atcttcgccg tgaggataca acaaacatta ttgcagtact gctccttgac attaaaggtt
     5521 taaacgatat caacaatatt ggtggacacc gtgaaggtga tcgcattttg aagcagctat
     5581 cacggctttt gaaaaccagc ctcagagaaa atgacatgat atttcgtaca ggcggcgata
     5641 tgtttttggc cataataaac tgcgacaatt attttgacct taagtcgctt gtggaacgca
     5701 ttgtacagaa agcaaacaat attaccctgc ctgaccaccc tgaacttcat attacaatac
     5761 accacgggct tgccattggt acacgcaatc gcattcagga actgattgaa gaagctcatc
     5821 gtcagaagat atagctatgt gctcctgagt ttgccaaaaa aatcctgtaa cagcttttta
     5881 cacttttctt cctgtatacc aaacattatt tcaggtatat ggtttaactg actgctacca
     5941 cacaccgtta gcacggtgcc acatgcccca aaacggtaat cccgggtgcc aatgaaaagc
     6001 ttttcaatgc gtgcatgtac gatagcgcca gcgcacattg cacacggctc tttggttaca
     6061 tataacaagc agcccgtaag acgctcattg tgtaaaaagt gtgccgcgtc tttgatggct
     6121 aatatttctg catgtgccgt ggggtcagag tattgccgcg ttttgttgtg agcacgtgct
     6181 atgactgttt catccttaa
//
LOCUS       DAORYL010000120         6186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_8762_length_6186_cov_11.461426, whole genome shotgun sequence.
ACCESSION   DAORYL010000120 DAORYL010000000
VERSION     DAORYL010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8762_length_6186_cov_11.461426"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            700..2586
                     /locus_tag="PLV81_14260"
     CDS             700..2586
                     /locus_tag="PLV81_14260"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOM11302.1"
                     /translation="MNKKVIFNITLMVVVSLVVAVFPLFNVFSNNNEQLLEDILGIKH
                     KDLYTLDTVANKAKWIDGQVIVKYKDTIATSKALQKLAKKAKHVSALHRKPLFNVILK
                     DMRVEDAIRELSKDPDVEYVQPNYIYHMLAVPNDPAFYMQWALKNTGQKVEGGYTINN
                     PGTLGCDANVEKVWDMHSDCSNIIVAVLDSGVNYIHQDLKDNMWDGSPYGYPYHGYNF
                     VYDDNDPMDYHGHGTHVAGIIGAKGNNNVGISGVCWKAKIMAVKVLTDEGYGSTSTIA
                     RGIYFAVDNGAKIINMSLGGSVYDTAFHFAINYAASKGVLVVAAAGNNGMGLYESMLN
                     PIYPCMFKSDNILCVGALDQNDIKATFSNYSYKDTVVHIYAPGVNIVSSFTSTVAEQI
                     TGNMINQSGWQMNDSNWAIHNTLGYTVASNPANFDFTVTYKPNQNSYLYRVVDCTGYK
                     KVSMIYEMYYDVENYYDAFRVYYAKGQQNPLYMNTSIFTLLATIAGKSDGFKTYMHNL
                     PNCRNALCTVVYNLSSDSVVNYKGVAIKSAGLYNLKPVDDRYAVLMGTSMATPMVSGA
                     AALVWAMFPDSNYKAIKERLINGAQKLLLYSGSFSYTINKLNVWGAVTYIKPPANVQY
                     KKIN"
     gene            2599..3009
                     /locus_tag="PLV81_14265"
     CDS             2599..3009
                     /locus_tag="PLV81_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11303.1"
                     /translation="MKKIIIPLFVIMFFIACSEYEEESANKNDDYLTYQYEISWDAND
                     EDGVNSFGGGYCVYYAEGYAVDINKSNYVDVPYQGGDRAPTQATIMIKLKKDENSNYK
                     RQVTYSVVVVAYATINGTKVYSRPSKQIQITLTI"
     gene            3036..3647
                     /locus_tag="PLV81_14270"
     CDS             3036..3647
                     /locus_tag="PLV81_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11304.1"
                     /translation="MSNHCFNLENRDLMFVRSTRSFVYLLRLAHVFGWKPKGTKLDPE
                     ERSLEEVCQWDGNYFLNEAQIVEEDDAMAIAQALEKAVTFLREARNLNMQATSHMYYS
                     EYIAVKPEQQKQQHINVDDIKIEGEDELLQENPEVEGNLNTFKNAVGAIFGFMYSKLE
                     DYDVADVIAFFDYYPDSDYDYNEVLESIEELITFCKAGAFRIC"
     gene            3702..4094
                     /locus_tag="PLV81_14275"
     CDS             3702..4094
                     /locus_tag="PLV81_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11305.1"
                     /translation="MTNGIDYNYPLETLFDYDATYDSLTDMHLQKNKHVYSMYDGDEF
                     YELIFSKDTKKLTVTDAKSQCNTCSYMHNCGCIVYHDCDEGRECCTYEERVWKAIDEY
                     EKEKLSEMGWYEFNGCIDCDDCDQHCLY"
     gene            complement(4159..4594)
                     /locus_tag="PLV81_14280"
                     /pseudo
     CDS             complement(4159..4594)
                     /locus_tag="PLV81_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002782504.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
     gene            complement(4596..5183)
                     /locus_tag="PLV81_14285"
     CDS             complement(4596..5183)
                     /locus_tag="PLV81_14285"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOM11306.1"
                     /translation="MNNSIYTIGHSNLEVEDFIQRLKRYSIQIVCDVRSNPHSAYVSH
                     FNALELRNKLTEYNIKYLYLGKELGPRSDNPQHYINGKVQYHLLEQSQHFKSGIQRLV
                     SGIQHFTIAIMCAEMDPINCHRSILICKHLPKEINIFHILGDSSLEAHSDLEKRLVIT
                     HFGDTATPFNTYDELLKQAYLLQAQKIAYTKEEEK"
     gene            5701..5895
                     /locus_tag="PLV81_14290"
     CDS             5701..5895
                     /locus_tag="PLV81_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11307.1"
                     /translation="MTKGIDYNFPLETLFDYTEIYDTLADMQLQYNKHLNEKKKNTFI
                     KYAIPGSKNHTKTKVPAIKR"
BASE COUNT         2067 a          869 c         1223 g         2027 t
ORIGIN      
        1 cacgtttagg tgatacacat agcgcttgtc gaagcacccc gctaataggg gattggggcg
       61 gatatgaaac ccgcccctac acataaatgg tgtaaaaccc cgctaatagg ggattttttt
      121 ttgtcttaat gtggaatgat ataccaatcg caggcgcttg agcacctgcg gtgttgtaat
      181 aaaatacaga gttgataaat gttgcttaat gattggaatg gccagatcct tcgtcatccc
      241 aaaaagacag ggactcctca ggatgacagt gtgggtatta cgttgtgatc tgtaataacg
      301 ttgatttcac atcacagaga gtaaagcgga aagtatacac gaggaatacc aagatccttc
      361 gtagtttcaa aaaggcctga actcctcagg gtgatggtat agatggtgta atgtaattat
      421 tgaagttcac agtgacaata ttgtttgttt acataactta aaggcggtat gacatatacc
      481 gcctttttta tttgtatcac tttaactata gtatatttcc ttgatcattg agttaacacc
      541 aattgaaggc tacatttact aaagaaaaaa aataacacat tttgcaaaaa aaattccaat
      601 aaaaataacc acaatactat caaataaatt aggtaatgtt atggtggatt gacttgttga
      661 gtaaaaagat attacaccta tgaaggaagg ttaaaaatta tgaacaaaaa agtgattttt
      721 aatattactc ttatggtagt agtttcatta gttgttgctg tgtttccttt gtttaatgta
      781 ttcagcaata ataatgaaca gttgcttgaa gatattttag gtatcaaaca taaagattta
      841 tatacactgg atactgttgc aaataaagct aaatggattg atggtcaggt tatagttaaa
      901 tataaagata ctatcgccac ttcaaaagct ttacaaaaac ttgcaaaaaa agcaaagcac
      961 gtatctgcat tacaccgcaa gccattattt aatgtaattc tgaaagacat gagagttgaa
     1021 gatgccattc gtgaattaag taaagatcct gatgttgaat atgtacaacc caattatata
     1081 tatcacatgc ttgctgttcc caatgatccc gcattttata tgcagtgggc attaaaaaat
     1141 acaggccaga aagtagaagg aggttacaca ataaataatc caggaacatt gggatgtgat
     1201 gcaaatgttg aaaaagtgtg ggatatgcat tctgactgca gcaatattat agtggctgtg
     1261 ttagatagtg gtgttaatta catacatcag gatttaaaag acaatatgtg ggacggttca
     1321 ccatacggtt atccatatca tggatataat tttgtttatg atgacaatga tccaatggat
     1381 tatcatggtc atggtacaca tgttgcagga ataatagggg caaaagggaa taataatgta
     1441 ggtataagtg gtgtgtgctg gaaggcaaaa atcatggcag tgaaagtgct cactgatgaa
     1501 gggtatggtt ccacttcaac tattgcacgg ggaatatatt ttgcagtgga taacggtgca
     1561 aagattatca atatgagttt gggtggaagt gtgtatgata ctgcatttca ttttgccata
     1621 aactatgctg ccagtaaagg tgtgctggtt gttgctgcag cgggtaataa tgggatggga
     1681 ttgtatgaaa gtatgttaaa ccccatatat ccctgtatgt ttaaatcaga caatattctg
     1741 tgtgttggtg cactggatca gaatgatata aaggcaacat tttcaaatta ttcatacaaa
     1801 gatactgttg ttcatatata tgcacctggt gttaatattg taagttcatt tacatccaca
     1861 gtggctgaac aaataacagg taatatgatt aatcaaagcg gctggcaaat gaacgatagt
     1921 aactgggcaa tacataatac cctggggtat accgttgcaa gcaatccagc aaattttgat
     1981 tttactgtta cctataagcc aaatcaaaac agctatcttt atagagtagt ggattgtacc
     2041 gggtacaaga aagtttcaat gatatacgaa atgtactatg atgttgaaaa ttattatgat
     2101 gcatttagag tgtattatgc aaaaggacag caaaacccat tatatatgaa taccagtatt
     2161 tttactttgc tggcaaccat tgctggtaaa agtgatggat ttaaaacata tatgcataat
     2221 cttcccaatt gccgaaatgc attatgcaca gttgtctata acctaagtag cgatagtgtg
     2281 gttaattaca aaggtgtggc aattaaaagt gcaggtttgt ataatcttaa gccagtggat
     2341 gacaggtatg cagtgttaat gggcacttca atggcaacac caatggtaag tggagcagcg
     2401 gcactggtat gggcaatgtt ccctgacagc aattataaag ccataaagga aaggttaata
     2461 aatggtgctc aaaagttatt attgtattct ggtagtttca gttataccat aaataaactt
     2521 aatgtatggg gtgcagtgac gtatattaag ccgcctgcaa atgtacaata taaaaaaata
     2581 aattaacgag gtggtttcat gaaaaaaatt ataataccat tatttgtcat tatgtttttt
     2641 atagcctgta gtgaatacga agaagaaagc gctaataaaa acgatgatta tttgacatac
     2701 cagtatgaaa tatcctggga tgctaatgat gaggatggtg ttaatagttt tggtggtggt
     2761 tattgtgtgt attatgcgga agggtatgca gtagatatta ataaaagcaa ttacgtagat
     2821 gttccttatc aggggggcga tagggctccc acacaggcaa ctattatgat taaactaaaa
     2881 aaagatgaaa atagcaatta caaaaggcag gttacttaca gcgtagtggt ggtggcatac
     2941 gcaacaataa atggcactaa agtgtatagc agaccttcta aacagattca aattactttg
     3001 actatataat aattatttac ctggaggttt taattatgtc aaaccattgt tttaatcttg
     3061 aaaacagaga tttgatgttt gttcgttcta cacgttcatt tgtgtattta ttacggcttg
     3121 ctcatgtatt tggctggaaa cctaaaggca caaaacttga ccctgaagag cgctctttag
     3181 aggaggtttg ccaatgggat ggtaattatt ttttaaatga agcacagatt gttgaagaag
     3241 atgatgctat ggcaattgca caggcgctgg aaaaagctgt tacgtttttg agagaggcaa
     3301 gaaatttgaa tatgcaggca acatcacaca tgtattacag tgaatatata gctgtaaagc
     3361 ctgaacaaca aaaacagcaa cacattaatg tggatgatat caaaattgaa ggtgaagacg
     3421 aattgctaca ggaaaatccc gaagtagaag gcaatctcaa tacatttaaa aatgcagttg
     3481 gcgcaatatt tggctttatg tattctaagc tggaagatta tgatgttgcc gatgtcattg
     3541 ctttttttga ttattatcct gatagtgatt atgactataa tgaagtactg gaatcaatag
     3601 aagaacttat tacattttgt aaggcaggtg cattcagaat ttgctaaata attgatgaac
     3661 acaagcctgt atatagggct tattaacagg gaggttacat tatgacaaat ggaattgatt
     3721 ataattatcc tttagaaaca ttatttgatt acgatgcaac atatgatagc cttactgata
     3781 tgcatttaca gaaaaataaa catgtgtact caatgtatga tggggatgaa ttttatgaat
     3841 taattttttc aaaagatacc aaaaagctaa ccgttactga tgccaaaagt caatgtaata
     3901 cctgctcata tatgcataat tgcggatgta ttgtatacca tgattgtgat gaaggaaggg
     3961 aatgttgtac ttatgaggaa cgtgtatgga aagcaattga tgagtatgaa aaggaaaaat
     4021 taagtgaaat gggttggtat gaatttaatg gctgtataga ttgtgatgat tgtgatcaac
     4081 attgtttata ttaaaaaata atatgtagtt gcattaaaaa acatttattt ttattaatgt
     4141 tgcttttttc gtgattgctt acaaatgtac aatatccaga ttataaaatc ttgcaaagta
     4201 ttcagcaacc agccgcctgt gacagttggt tggctcatat tcactgcata aaaggcaggc
     4261 ggtatgcaaa ttgtgttcgt tatagtagtg ctcaacattc cgctgtgtaa gaagttgtac
     4321 aaatttttcc tgatactgtt cccagcttat tgtttttttt gtgtattgca tcaagcattt
     4381 cttttgttgg cgccaggcgc acatcgtgaa tatatttgat gtgtgctatt tcccataaaa
     4441 aatattccag atcttctttc tttgtgtatc cacttagttg tgatgtattg ttaattctaa
     4501 tgtcaatgac tgttgttatg ccattgtttt gtaatgtggt aaaaaaatca cgggcatttc
     4561 gttttgtaaa tcctattgtg tataatgttg ccattttact tttcctcttc tttagtatat
     4621 gctatctttt gtgcctgtaa tagatatgcc tgcttgagta attcatcata cgtgttgaag
     4681 ggtgttgcgg tatcgccaaa atgtgttatt accaggcgct tttcaagatc tgagtgtgct
     4741 tccaatgaac tatcgcccaa aatatgaaat atattaattt cttttggtaa atgtttacaa
     4801 ataaggattg acctatgaca attaatggga tccatttccg cacacattat ggcaatggta
     4861 aaatgttgaa ttccagatac aagtctttgt atgccgcttt taaagtgttg gctttgctct
     4921 aaaagatggt actgcacttt tccatttatg tagtgttgag gattgtcaga tcgtggacca
     4981 agttcttttc ccaggtacag gtatttaatg ttatattcag taagtttatt gcgtaactcc
     5041 agtgcgttaa aatgagaaac gtatgcacta tgtggatttg aacgtacgtc acaaacaatt
     5101 tgtatggaat aacgttttaa acgttgtata aaatcttcaa cctcaagatt ggaatggcct
     5161 attgtatata tactattatt cattgtaatt ccccggatta catgttagtt tgcttgtata
     5221 attgatgcca gtaatttata tctatagcca taaaaatctt caccaagcga tatacctgta
     5281 tatacagaac ctttcatagt ggtatatata tattgctgtg ttttcgtgat aatgctaagc
     5341 ataccattgt ggttttcatg atgtttattt acctccaggt gtattggatt aatctgatac
     5401 atgcttaatg gtgtggcttt ttggattgat atagtgttat gcgtgtacta tttattagaa
     5461 ggattacgat aaaaaattat tgggattttt tgttatgcct gaggtaattt ttgtcgtttt
     5521 agcattgtaa aagtttaatt tctgaataaa aataaataaa caataaaaag tcctaacgct
     5581 ttcatatgtg gctaacaacc ttgctttatt aaaaattttt cataaataaa aaaaattcca
     5641 atattttcaa agcttttcaa ttcaaataaa cataaccctt taacataagg aggtttttac
     5701 atgacaaaag ggattgatta taactttcct ttagaaacat tatttgatta tactgaaatc
     5761 tatgatactc ttgctgatat gcagttgcag tacaataaac atctaaatga aaagaaaaag
     5821 aatacattta tcaagtatgc aatacctggt tcaaagaatc ataccaagac aaaagttcct
     5881 gctataaaac gttaatgtaa ttcatgcaca tatatggcta catgcaatgt actgtatagc
     5941 atcattgtga tgatggtaga aaatgatgta ccgctgagca gcatgcattg aaagtgtttg
     6001 attattatgt aaaggaaaaa gtaaatgaaa tgggttggtt tgaaatatat gaatctttac
     6061 tttgtgatca ttgcaattat cgctgtttat ttaaggaaga aaggagatgt agtatttcaa
     6121 tgtacattat agtgaatatt attataaaag aatctatatt gaatataaaa aaattcaata
     6181 acaaaa
//
LOCUS       DAORYL010000121         6121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_8878_length_6121_cov_12.232113, whole genome shotgun sequence.
ACCESSION   DAORYL010000121 DAORYL010000000
VERSION     DAORYL010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8878_length_6121_cov_12.232113"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(423..1934)
                     /locus_tag="PLV81_14295"
     CDS             complement(423..1934)
                     /locus_tag="PLV81_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005984199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HOM11308.1"
                     /translation="MEFLNKLIQKIERKHIVIAIGAVIALSLFIYIIGALGPSEPKLV
                     LVGTIPDNEYDPQEWGKVYPLEYESWKKTQDPRPADKSFYKKGWDTDKIIYDKLSEYP
                     FMALLFKGWGFGIAYNEPRGHWYMRIDQDEIDQSRTKAGGVCLNCKTPYMDALVKQYG
                     NSFYSMKWQDAIAKIPPKHKDLGATCIDCHDNKTMGLKINREAFKKGLAKLGKKEYTR
                     QEMRILVCAQCHVTYNIPKNEQMQSIGLEHPYEESSWGNISIENIIRLIKKNANWGEW
                     KQQVTGFKVGFIRHPEFEFFTRQSVHFNAGIACADCHMPYKRVGTFKISDHNLMSPLK
                     DDLFACLKCHPQSKERLVKQIKEIQDRTISLLLKAGYHEAVVAKLFERIHNQFKDTLA
                     SDPLYVQAKDFYLEALYRLIYMGAENSIGFHNPEEAGRILGDSIGFSTKAEGLLRQLL
                     AKKGIDVGQSINLELAKYLNNRGEHKLMFKREQEFKDPFGIVEKTVPRSSMGL"
     gene            complement(1957..2385)
                     /locus_tag="PLV81_14300"
     CDS             complement(1957..2385)
                     /locus_tag="PLV81_14300"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HOM11309.1"
                     /translation="MKYKVYTVFGIFIILLAFGVYGTARFMQKTSTPQYCGSCHVMIP
                     QYEDWFYIGKHTQIACVDCHLPHNNSLNYVLWKGLDGMKDVVLFYTRLYGEVIHSSAH
                     ARKTIQANCIRCHSEMVSRIDGGAIQCWDCHRYFYHNIIH"
     gene            complement(2557..3249)
                     /locus_tag="PLV81_14305"
     CDS             complement(2557..3249)
                     /locus_tag="PLV81_14305"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HOM11310.1"
                     /translation="MIVCIQHVPFETPGYILDWAIVHQKDVAIVTAYTNHDFPVVDSV
                     EMLVIMGGPMSVHDEREYPWLVTEKHFIEKVINKGKPVLGICLGAQLIADVLGARVYK
                     NHTKEIGWFEVQRVQSENPFSKLLPDTFMAFHWHGETFDIPAGAIHIASTDVCANQAF
                     VIDDRIVGLQFHLEITAQGVYDLVANCGAELKENGHIQSKDAIIAGISYCKHTHALMD
                     SLLEAFTMSYRT"
     gene            complement(3252..3572)
                     /locus_tag="PLV81_14310"
     CDS             complement(3252..3572)
                     /locus_tag="PLV81_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009436917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOM11311.1"
                     /translation="MSNLFNDPQNFVNAIEYQSNAIVSKTIIQNKSGSLTLFAFGQGQ
                     SLSEHTAPYDAFVMVLDGQFIITIGGKENTVKAGEAIIMPANVPHALAAANNAKMLLV
                     MIRG"
     gene            complement(3593..4915)
                     /locus_tag="PLV81_14315"
     CDS             complement(3593..4915)
                     /locus_tag="PLV81_14315"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOM11312.1"
                     /translation="MQGEIKLIQNKQKKVVIIGAGFGGLWVAKTLANTGFHVTIIDKN
                     NYHTFLPLLYQVSAAEVAPEQIASPVRNIVRKHNNIQFIRGAVSKIQYSTKTVLCLGQ
                     EIPFDYLIVSAGSENNYFNIPGVARYAFPLKTLDDAMVLRNHILTCFERASFVNDEKQ
                     RKRLLTVVIVGGGPTGVEFAGALAELIAGPLKKDFPELQHEEITVYLIEASDRLIGMY
                     PEKLGDYTCKQLQKKGVKVVLNAAVTNIDATGVQLNNGGIIPTETVVWTAGVKGELIA
                     NDLNVKTMPNGRVIVDEYCRVPGYDDVFIIGDLACFMHNGKPLPMIAPVAMQQGRYVG
                     KHLLQKLKGKTPKPFRYRDKGGMVAIGRNKAIAQIAGLHVKGYIAWILWIFIHILYLI
                     GFKNKIFVMINWVYSYIFFEKSVRLILPRCCDDPHGDACLQRGRSCKG"
     gene            complement(4915..>6121)
                     /locus_tag="PLV81_14320"
     CDS             complement(4915..>6121)
                     /locus_tag="PLV81_14320"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HOM11313.1"
                     /translation="EDYMSEFLTDMGKKKEQLKDMIKKLHEGADVNEMKAIFKKEFGN
                     VMPHQISMLEEELIKEGMPSDEVHRLCQLHIDLFRESIDAGKVDVPEGHPLYILYTEH
                     SRILEFAQQLVESATRLFDTPDSATFARIQQLIGFFKESAVHYLREENVLFPVLEKYG
                     VTQPPKIMWMEHDNIRAIEKSIYETADALAKGITKELSHTLQTHAIRLSETLADHFYK
                     ENNILFPTSYKFFNEDDWKLVKQEFDGIGYMSYSPVIHIEKGEGAGTRLASDTVEFET
                     GNLTLEQLQYMLDTMPVDFTFIDKDDVVRYFNNAPDRIFVRSKAVIGRTVQNCHPDKS
                     IHVVNRLLDEMKQGKRNKAEFWITLNGRLIFITYYAVRKNGEYIGCLEVTQDITDIQK
                     ITGQKRLLD"
BASE COUNT         1831 a         1402 c          947 g         1941 t
ORIGIN      
        1 cctttgattt ttaccgtgaa aacggcatcc attgcttaaa attctactgg ggaccaccac
       61 ctgaaatact aacacaagct taattaatca aattcaatat tgaaaaaatg catatgttaa
      121 tccttattac cgtaaggtca aaaaaacttt tgtttttggt ttaagatgtc attctagata
      181 gtagaatttt gctttcaaaa atattacagt atatttgcag ccaagaatga catctacagc
      241 attattttca ccaattgaga atcacgtaac taagttcaca taattataac aaaataatta
      301 aacgtaaaga taataaaact ctactactta taaaatcaga attacaaaga ctaaaattaa
      361 tttatcatta tgcacatacc aatttatcca tctatacagc atgtcacata aaaaattaat
      421 ctctacaatc ccatgctact gcgtggaacg gtcttttcta ctataccaaa tggatcctta
      481 aattcctgct ctcgtttaaa catcagcttg tgttcaccac ggttgttaag atactttgca
      541 agttctaagt taatactctg tccaacatca atgccttttt tagccagtaa ctgtcgcaac
      601 aaaccttctg ctttagtaga aaatccaatg gaatcaccta atattcttcc agcctcttct
      661 ggattatgga atccaattga attttctgct cccatataaa tcaaccgata caacgcttct
      721 aaataaaaat ccttagcctg cacatataat ggatcagaag caagcgtgtc cttaaattga
      781 ttgtgaatac gctcaaaaag ctttgcaacc accgcttcat ggtatccagc tttcagcaac
      841 agtgatatgg tcctgtcctg aatttctttt atctgtttta ccaatcgttc cttggattga
      901 gggtgacatt tcaaacaggc aaataggtca tcttttagcg ggctcataag attatggtct
      961 gaaatcttaa aggttcctac tcttttatat ggcatgtgac aatcagcgca agctatcccc
     1021 gcattaaagt gaacactctg tcgtgtaaaa aattcaaact caggatgccg tataaatcca
     1081 accttgaaac ctgtgacctg ctgtttccac tcgccccagt tagcattctt ttttataagt
     1141 ctaatgatat tttcaattga aatatttccc cagcttgatt cttcatatgg atgttcaagc
     1201 ccaattgatt gcatttgctc attctttggt atattgtagg taacgtggca ctgtgcacat
     1261 accagaatgc gcatctcctg cctggtatac tctttttttc ctaattttgc taaccccttt
     1321 ttaaatgctt ccctgttaat ctttaatccc atagttttgt tgtcatggca atcaatacag
     1381 gttgcaccca ggtccttgtg ttttggaggt attttggcga tagcatcctg ccacttcatg
     1441 ctgtaaaaag aatttccata ctgtttaacc agtgcatcca tatacggcgt tttgcagttt
     1501 aagcagacac caccagcttt tgttcttgac tgatcaattt catcctgatc aatacgcatg
     1561 taccagtgcc cacgtggttc attatacgca atgccaaaac cccagccttt aaaaagcaat
     1621 gccataaatg gatattctga taatttatca tatataattt tatctgtatc ccatcccttt
     1681 ttataaaagc ttttgtctgc tggtcgtgga tcctgtgttt tcttccatga ctcatattcc
     1741 agcggatata ccttgcccca ctcctgtggg tcatactcat tatcaggaat agttccaaca
     1801 agcacaagct ttggttctga gggcccaagc gctccaataa tataaataaa aagtgataag
     1861 gcaataacag caccaatggc aataacaatg tgtttgcgtt caattttttg aataagtttg
     1921 tttaaaaatt ccattgctaa cctccagtaa aaatttttag tgaataatat tgtgataaaa
     1981 atacctgtga caatcccagc actgaattgc cccaccatca atacgtgata ccatttcact
     2041 atggcaacgt atgcaattag cctgtattgt tttacgcgca tgtgccgaag aatgaatcac
     2101 ttcaccatac aaccgtgtat aaaaaagcac cacatccttc atgccatcaa gccccttcca
     2161 taaaacataa ttcaggctgt tattgtgtgg cagatgacaa tcaacacagg caatctgtgt
     2221 atgcttacca atataaaacc agtcttcata ctgaggaatc attacatggc agctaccgca
     2281 atactggggt gttgatgttt tctgcataaa cctggcagtg ccatatacgc caaatgctaa
     2341 gagtataata aatattccaa atactgtata tactttatat ttcatggctc acctcaatca
     2401 aaaataaaat tacaccaaac atcattgtgg taaactcaag tactcataca atcgtatcgt
     2461 aaaaatgcga tagtatgtcc atcatgacgt tttcccttta ttttattttt ctatcctgac
     2521 agcggtaatt gacaacacct ccagaagcat tttacattat gtgcgatagc tcatggtaaa
     2581 agcttcaagc agtgaatcca ttaatgcatg cgtatgtttg caataactaa tacctgctat
     2641 aatagcgtct ttactctgaa tatggccatt ttccttaagc tcagccccac aatttgctac
     2701 cagatcataa acaccctgtg ctgtaatctc cagatgaaat tgcagcccta ctattctatc
     2761 atcaatgaca aatgcctgat tggcacatac atcagtagat gctatatgta ttgcacctgc
     2821 aggtatatca aatgtttcac catgccagtg aaatgccata aatgtatcag gcaacagttt
     2881 gctaaatgga ttttctgatt gtactcgttg tacttcaaac cacccaattt cttttgtatg
     2941 atttttatac actcgtgctc ccagtacatc agcaataagc tgtgcaccaa gacaaatccc
     3001 taaaacaggt ttgcctttat ttattacttt ttctataaaa tgcttttctg taacaagcca
     3061 tgggtattct cgttcatcat gaacactcat aggcccaccc ataattacaa gcatctcaac
     3121 tgaatcaaca actgggaaat catggtttgt atatgcagta actatcgcca catctttttg
     3181 atgcacaatt gcccaatcca gtatatagcc tggcgtttca aatggtacat gctgaataca
     3241 tactatcatg attatcctct tatcataaca agaagcatct ttgcattatt tgctgcagca
     3301 agggcatggg gtacattagc tggcataatg atagcttccc ctgctttcac agtattttct
     3361 ttgccaccaa tggtaatgat aaattgacca tcaagcacca ttacaaaagc atcatacggt
     3421 gcagtatgct cgctcagact ttgtccctgc ccaaatgcaa agagagttaa actaccgctt
     3481 ttattctgta ttatagtttt gctcacaatt gcattgcttt gatattcaat tgcattaaca
     3541 aaattttgtg ggtcattgaa aagattactc atagtattcc tcccatacaa tgttatccct
     3601 tacaacttct tccacgttgc aagcacgcat ccccatgagg atcatcacaa catcgtggca
     3661 aaataagccg cacacttttt tcaaagaaga tataactgta tacccagtta atcataacaa
     3721 aaattttatt tttaaaacca atcaaataca aaatatgaat aaagatccat agtatccatg
     3781 caatatatcc cttcacatgc aaacctgcaa tctgagcaat agctttatta cgtcctatag
     3841 caaccatccc acctttatcc ctatacctga aaggtttggg tgttttgcct ttaagctttt
     3901 gcaataaatg tttgcccaca tatcttccct gctgcatagc aactggtgca atcatgggaa
     3961 gtggtttgcc attatgcata aaacaggcta aatcaccaat aataaacaca tcatcatatc
     4021 caggcacccg gcaatattca tcaactatca cccttccatt gggcattgtc ttaacattca
     4081 aatcatttgc tataagttca cccttaacac ctgctgtcca caccaccgtt tcagtgggta
     4141 ttatgccacc attgttcaat tgtactcctg ttgcatcaat gtttgttacc gctgcattga
     4201 gtacaacctt tacccctttc ttttgcagtt gcttgcaggt ataatcacct aatttttccg
     4261 gatacattcc aataagtcta tcgcttgcct caataagata cacagttatt tcttcatgtt
     4321 gcaattcagg aaaatctttt ttcaatggtc ctgcaatcaa ctccgcaaga gcaccagcaa
     4381 attcaacacc tgttggacct ccaccaacaa tcaccacagt aagcaatcgc tttcgctgtt
     4441 tttcatcatt cacaaacgaa gcccgttcaa aacaggtaag gatatgattt cgcaaaacca
     4501 tggcatcatc cagtgttttc aaagggaacg cataacgagc aacacccgga atattaaaat
     4561 aattgttttc actacctgct gatacaatca gataatcaaa cggtatttcc tgacccaggc
     4621 acagtacagt ctttgttgaa tactgaatct tactaacagc accacgtata aactgtatat
     4681 tattgtgctt tctaacaata ttgcggaccg gtgatgctat ctgttccggt gctacttccg
     4741 cagcactcac ctgatacaaa agtggcaaaa atgtgtgata gttattttta tcaatgattg
     4801 tcacatgaaa accagtattt gctaacgtct ttgcaaccca aagcccgcca aagccagcac
     4861 caatgataac aacttttttt tgtttgttct gtatcaattt aatttctccc tgcattaatc
     4921 caataacctc ttctgcccgg ttatcttctg aatgtcggta atatcctgag taacttccag
     4981 gcagccaata tactcaccgt ttttacgcac agcatagtag gtgataaaaa tcaaacggcc
     5041 atttaacgta atccagaact cggctttatt gcgcttaccc tgcttcattt catccagcag
     5101 gcggttaacc acatgaatgc ttttatccgg atggcagttt tgcacagtcc tgccaataac
     5161 agcctttgat cgcacaaaga tacggtcggg cgcattgtta aagtagcgta ccacatcatc
     5221 tttgtcaata aatgtaaaat ctaccggcat ggtatcaagc atgtattgaa gttgttccaa
     5281 cgtcaggttc cccgtttcaa attccacagt atcagatgcc aaacgtgtgc ctgcaccctc
     5341 acccttttct atgtgaataa ccggactata tgacatatac ccaataccgt caaattcctg
     5401 cttaactaat ttccagtcat cttcattaaa aaacttatat gacgttggga acaatatatt
     5461 gttctctttg taaaagtggt cagcaagcgt ttcactaagc cgtatggcat gtgtttgtaa
     5521 tgtgtgcgat agttcctttg taatgccctt tgccagtgca tcagctgttt catatatgct
     5581 tttttcaatt gcacgaatgt tgtcatgctc catccacata atctttggcg gttgagtaac
     5641 tccatatttt tccagaaccg ggaaaagcac attttcttca cgaagatagt gcaccgctga
     5701 ttctttaaaa aaaccaatta actgttgaat gcgtgcaaac gtagcactgt ctggtgtatc
     5761 aaaaagtctg gttgcacttt caactaattg ctgcgcaaat tcaaggatcc tgctatgttc
     5821 agtatacaga atatacaaag gatggccctc gggcacatcc actttgcctg catcaattga
     5881 ttctctgaaa agatcgatat gcaactggca cagcctgtgc acctcatcag atggcatgcc
     5941 ctctttaata agctcttctt ccagcatgct tatctgatgc ggcatcacat tgccaaattc
     6001 ttttttaaag atagccttca tctcattgac atctgctccc tcatgaagct ttttaatcat
     6061 atctttaagc tgctccttct ttttacccat atctgttaaa aattcgctca tgtaatcctc
     6121 c
//
LOCUS       DAORYL010000122         5742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_9598_length_5742_cov_13.174433, whole genome shotgun sequence.
ACCESSION   DAORYL010000122 DAORYL010000000
VERSION     DAORYL010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9598_length_5742_cov_13.174433"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3149
                     /gene="recC"
                     /locus_tag="PLV81_14325"
     CDS             <1..3149
                     /gene="recC"
                     /locus_tag="PLV81_14325"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOM11314.1"
                     /translation="QFGVFLNCRFPFPENFAQEIFKAVTPDYELSPLYTNKSLVWLIV
                     SLLPQCLDEKPFEPVKQYLVVDGTISQVRLYQLAHRLAYMYDEYSVYFYRDILQWEKE
                     PSNAWQVLLWRYIKNNISTDSKICHKAQLLEHVIKALNDPKNTQVLKEKLADEVFLFG
                     ITVLPEYYITLFNVVSSIVPVHIFQLNPSKEYWFDIRSEKALLRIQQKIKAKNININD
                     LHFEPGNPLLASLGKVGQEYIGLLLDYNAYDTITDEGQYTIRNSSMLLAHIQNDILHC
                     IERGNGKHEKMAISPDDNSITIHSCHSPLREIEVLRDYIINILNTTAIVPNDIVVMAP
                     DIDVYAPFIKAVFDEQVLHREGLPVLPYAIADQSYKNTSRFISAFSDVLEVFAGRLEA
                     DKVCELLDYEEITQRFGIEKHYVMAFHQWVEELNVRWGKDASHRKQYTHTESNAFTWQ
                     YAVERLLMGYAVPQAAGLVDDVIPFDSVEGSSAVVLGCFLNFLEALFRFFDEAQKPKK
                     LEDWINFYDNLINTMLDVESHLQDYENIQQLLSSLRAIVTDINLNEDIEFLVIKSWID
                     DFLSEQRVSSNFLSKGITFCQLLPMRSIPFKVVCLLGMNDGEFPRLDEHISFDILRNE
                     THKDTLPRCIRSKRNDDRYLFLESIISAQEYLFISYQGQNPKDLSLKEPSVVVSELLE
                     YIERGFYVQDQSTSIRDVFITRHHLHHFHPAYFQEHRKYFSYSRLWFEESRALYGKKE
                     DYKPLISQLDLPIKITDITIDELIQFFKNPARHFITKVLCIYLRDEVIELAGDELLSI
                     SDNLDNYILDNEIVTWKIDGNDIHQFTYIKKKEGIIPDGMLGDIIVKKKDAEVAYFAN
                     KVKQYCIGERQSYELAYTSEKGITITGKPVLYGGNQVFYRYANEKVRDRLQAFLWHIF
                     VCATMELGVTTYLIVKNKVGPIVFGNISCDQAKTYLEKCIDIFIEGNKRILPFFPETS
                     GEYYKLMNKDKGRFIQYKIEGKFYEENAYNIPDYSEPYIRRAFYKVDIFRNKEVFYEF
                     TELAEEIFTMIQECERSQ"
     gene            3146..>5742
                     /locus_tag="PLV81_14330"
     CDS             3146..>5742
                     /locus_tag="PLV81_14330"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOM11315.1"
                     /translation="MNNPCSVNNFWEYSLDGINCIEASAGTGKTYTISHLYLRLIIEK
                     GLSIDNILVVTFTNAATDELRSRIRSLIKIAYDMLEGRCEDAVKQKYSELYEYLQSLS
                     TNNSSNYRTQKEIRDILKIALADIDKAHIYTIHSFCSRTLSEFAFESRSLYGSKLVED
                     ETPFMQRVIEDFYYSKIAPLGEEFVALFFQKNENFVRWFMEILRKLPDDPDITIMPVI
                     PFNPKEVKQLVHTLTMQIGAIREKFNREFDAIKNNYFKALAERVLNSRSYNEEDLLTL
                     QQAIEMLSEFSVFQEDISKHIKKFSVSGLVSRLIGAYKKNEKQYTWHEFFTLLDECRK
                     TIVAIKIQIIAAYREFIQYSEKQYSWYRDRWNIISFRDILKDMHKALQQGTDSRLAVR
                     VRERFKAALIDEFQDTDPLQYTIFTTIFRKEGYTLYIIGDPKQAIYSFRGADVFAYIK
                     ARQSADTAYTLTKSFRSTPSLTRAINYIFGRVENPFVFDEIMYHPVESKIHESCFSHG
                     ALDIWFIRDSDNEKSISIGVARKIIAKNIAHEIHTLVHGSYEIDGKALQYSDIAILVR
                     KHHEAELMQDILRQYGINSVLYSRQSVFTTPEVWELLHVLEAIINPSSTKAIKRALVT
                     SLFGYCGNDIIQMEEQGTLDTVKMRCAAYLDTWKAKGVIVMLQRLLHDDVIVKKENER
                     DTVARRLLQYYDGERVITNILHCAEILADTERQKKLEPVALVEWLKKAIYVGADTEEQ
                     QLRLESDANAVKIVTIHASKGLEYPIVFCPYLFDASSLDRIFVFHDSTDNNYKTHVSF
                     RFSKEDVEEMKSLFPDNYYNEMEYIAKREQLAESLRIAYVAMTRAKFKCYVGWGNINS
                     TETSALRYL"
BASE COUNT         1926 a          896 c         1160 g         1760 t
ORIGIN      
        1 agcagtttgg tgtgttttta aactgcagat tccctttccc ggaaaacttt gcccaggaaa
       61 tttttaaggc agtgactcct gactatgaac tatcgccact gtatactaat aaatctctgg
      121 tgtggcttat tgtttcatta ttgccacagt gtttagatga aaagccattt gaaccagtta
      181 agcagtatct tgtagttgat ggcacaataa gtcaggtgcg attatatcag cttgcccaca
      241 ggcttgccta tatgtatgat gaatatagtg tgtattttta cagggatatt cttcagtggg
      301 aaaaggaacc atcaaatgcc tggcaggtgt tgctgtggcg atatatcaaa aacaacattt
      361 ccactgattc aaaaatttgc cataaggcgc aacttcttga acatgttatt aaagcgttaa
      421 atgatccaaa gaatacacag gtgttgaagg aaaaattagc tgatgaggta tttttgtttg
      481 gcattaccgt gttgccagag tattacatta cactattcaa tgttgtaagc agcattgttc
      541 ctgttcatat ttttcagctt aatccatcaa aggaatactg gtttgatata cgcagtgaaa
      601 aagcactatt gcgtatacaa caaaagatta aggctaaaaa tattaatata aacgatttgc
      661 actttgagcc agggaatcca ctgctggcat cgctgggtaa agtggggcag gaatatatag
      721 gtctgcttct tgattataat gcgtatgata ctatcactga tgaaggtcaa tatacaattc
      781 gcaatagttc aatgttacta gcccacattc aaaatgatat attacactgc attgaacgtg
      841 gaaatggtaa gcatgaaaag atggcaatat cccctgatga taattcaata acaattcatt
      901 catgtcattc tccactgcgt gagattgaag ttctgcgtga ttatatcatc aatatattaa
      961 acactactgc aattgttcct aatgacattg ttgtcatggc acctgatata gatgtgtatg
     1021 caccattcat taaagctgtg tttgatgaac aggtattaca cagggaagga ttgcctgtat
     1081 tgccgtatgc tattgctgac cagtcatata aaaatacaag ccgttttatt tcagcatttt
     1141 ctgatgtact tgaagttttt gcaggcaggc ttgaagctga taaggtttgt gagcttcttg
     1201 actatgaaga aataactcaa agatttggaa tagaaaaaca ttatgttatg gcttttcatc
     1261 agtgggtgga agaactcaat gtacgatggg ggaaggatgc ttcccatcgc aaacaatata
     1321 cacacactga aagtaatgca tttacctggc agtatgctgt tgagcgctta cttatggggt
     1381 atgctgtgcc acaggcggca ggccttgttg atgatgttat cccttttgat agcgttgaag
     1441 gttcatcggc tgtagtactg ggatgttttt taaattttct tgaagcgcta tttagatttt
     1501 ttgatgaagc gcaaaaacca aaaaaactgg aagattggat aaacttttat gacaatctca
     1561 taaacaccat gcttgatgtg gagagccatt tgcaggatta tgaaaatatt caacaattgt
     1621 tgtcatcatt acgggcgata gttactgaca ttaatcttaa tgaagatata gagtttttgg
     1681 taataaaaag ctggattgat gattttcttt ctgagcaaag agtcagcagt aattttttaa
     1741 gtaaaggcat taccttttgc cagcttctgc ccatgcgaag tatccccttt aaggttgtat
     1801 gcctgcttgg tatgaatgat ggagagttcc cacgacttga tgaacacata agctttgata
     1861 tattacgcaa tgaaacacac aaagatacat tgccacgatg cattcgttca aaacgcaatg
     1921 atgaccgtta tttatttctg gaaagcataa tttcagcaca ggaatatcta tttataagct
     1981 atcagggaca aaacccaaag gacttaagcc ttaaagagcc atctgtggtt gtcagtgaat
     2041 tgcttgaata tattgaacgt ggattttatg tacaggatca atctacatca atacgtgatg
     2101 tctttattac caggcaccat ttacatcact ttcatcctgc atattttcag gaacacagga
     2161 aatatttttc ctattcaaga ctgtggtttg aagaaagcag ggcgttatat gggaaaaaag
     2221 aagattacaa accactaatt tcacagctag atttgccaat aaagattact gatattacta
     2281 ttgatgaact tattcagttt tttaaaaacc cagctagaca ttttattaca aaagttctat
     2341 gtatctattt gcgtgatgag gtaatagagc ttgcaggcga tgaactgttg agcatttcgg
     2401 ataatctgga taactatata ttagataatg agatagtaac ctggaaaata gacggtaatg
     2461 atatacatca atttacgtat atcaaaaaga aagaaggaat tatccctgat ggcatgttag
     2521 gcgatattat tgttaaaaag aaagatgcag aggtagcata ttttgcaaat aaggttaagc
     2581 aatattgtat aggtgaaaga caaagttatg aattggcata tacatcagaa aaaggaataa
     2641 caataacagg caagccagtg ttatatggtg gaaaccaggt tttttacagg tatgccaatg
     2701 aaaaagtaag agataggctg caggcatttt tatggcacat atttgtatgt gccactatgg
     2761 aacttggagt tacgacatat cttatagtca aaaataaagt aggaccaata gtttttggaa
     2821 acatttcttg tgatcaagca aaaacctatt tagaaaaatg tattgatata tttattgaag
     2881 ggaataaacg cattttgccg tttttcccag aaacctcagg tgaatattat aagttaatga
     2941 ataaggataa gggaagattt attcaatata aaattgaagg aaaattctat gaggaaaatg
     3001 catataatat ccctgattat tcagagccat atatcagacg agcattttac aaggttgata
     3061 ttttcagaaa taaagaagta ttttatgaat ttactgaatt agctgaagaa atatttacta
     3121 tgatacagga atgtgaaaga tcacaatgaa caatccttgc agtgttaaca atttttggga
     3181 atattcttta gatggcatca actgcattga ggcaagtgca ggtacaggaa aaacctatac
     3241 cataagccat ctgtatttac ggcttatcat tgaaaaagga ctcagtattg ataatattct
     3301 tgttgtaaca tttaccaatg cagcaactga tgagcttagg tcacggatac gttctctcat
     3361 aaaaattgca tacgatatgc ttgaaggaag atgtgaagat gctgtaaagc aaaaatacag
     3421 tgagttatat gaatatctgc aatctttatc taccaacaat agcagtaact atcgaacaca
     3481 aaaagagata cgtgatattt taaaaatagc gcttgctgat attgataaag cacatattta
     3541 taccattcac agtttttgca gccgcacatt aagtgaattt gcttttgaaa gccgttcatt
     3601 gtatggaagc aaacttgttg aagatgaaac acctttcatg caaagagtca ttgaagattt
     3661 ttattattct aagatagcac cgcttggaga ggagtttgtt gcattatttt ttcaaaaaaa
     3721 tgaaaacttt gtcaggtggt tcatggaaat tcttcgtaaa ctgcctgatg atcctgatat
     3781 taccatcatg cctgttatac cgtttaaccc aaaagaagta aaacaactgg tacatacgct
     3841 aacaatgcag attggagcta ttcgtgaaaa atttaatcgt gagtttgatg caataaagaa
     3901 taattatttc aaagcactgg cagagcgcgt cctgaatagc agatcgtata atgaagaaga
     3961 tttactgaca ttacagcaag ctatagaaat gctttctgaa ttcagtgtgt ttcaggaaga
     4021 cattagtaaa catataaaaa aattttcagt ttcaggtttg gtgtccaggc tgataggtgc
     4081 atacaaaaaa aatgaaaagc aatatacatg gcatgaattt tttacacttc ttgatgaatg
     4141 cagaaaaact attgttgcta taaagataca gataatagcc gcatatcgtg aatttatcca
     4201 gtacagtgaa aaacagtact catggtatcg cgatcggtgg aatatcattt cattcaggga
     4261 tatcctgaaa gatatgcata aagcactaca gcaaggtacg gacagcagat tggctgttcg
     4321 agttagggag cgtttcaaag cagcactcat tgatgaattt caggatactg atccgttaca
     4381 atatactatt tttaccacaa tattcaggaa agaaggttat accctgtata tcattggtga
     4441 tcccaaacag gcaatttata gcttcagagg agctgatgtt tttgcgtata ttaaagcacg
     4501 tcaaagtgca gatactgcgt atacattaac taaaagtttc agatcaacac catcgttaac
     4561 cagagctata aattatattt ttggtcgagt tgaaaacccg tttgtatttg atgaaattat
     4621 gtatcatcca gttgaatcta agatacatga gtcatgtttt agccatggag cattagacat
     4681 atggtttatt cgggatagcg ataatgaaaa gagtatatct attggtgtag cacgtaagat
     4741 aatagccaaa aatatagccc atgaaattca tacattagtg catggttcct atgaaattga
     4801 tggcaaagca ttacaataca gtgatatagc aatactggta cgaaagcatc atgaagcaga
     4861 actgatgcag gatattttaa gacagtatgg tattaatagt gttttatata gcagacagtc
     4921 agtatttact acacctgaag tgtgggaatt gttgcatgtg cttgaggcaa ttataaatcc
     4981 ctcatcaaca aaagccataa agcgggcact ggtaacaagc ttatttgggt attgtggaaa
     5041 tgatattata caaatggaag aacagggaac attggatact gtgaagatgc gctgtgctgc
     5101 atatcttgat acctggaaag caaaaggtgt tattgttatg ctgcagcgcc ttcttcatga
     5161 tgatgtgata gtaaaaaaag aaaatgagcg cgataccgtt gccaggaggc ttttgcaata
     5221 ctatgatggt gaaagggtga taaccaacat actgcactgt gctgaaatac ttgccgatac
     5281 tgaacgccag aaaaaattag aacccgtagc actggttgag tggttgaaaa aggccatata
     5341 tgtgggagct gatactgaag agcagcaatt acgtctggaa tccgatgcca atgctgttaa
     5401 aattgttact atacatgcaa gtaaagggct ggagtatcct atagtttttt gtccttatct
     5461 ttttgatgct tcttcattag atagaatatt cgtttttcac gatagtacag ataataacta
     5521 caaaactcat gtgtccttca gatttagtaa agaggacgtt gaggaaatga aatcattatt
     5581 cccagataat tattataatg aaatggaata tattgccaaa cgtgaacaac ttgctgagtc
     5641 tctgcgaatt gcctatgttg caatgacgcg tgcaaagttt aaatgctatg ttggatgggg
     5701 aaatataaat agtacggaaa catcagcttt gcggtacctt tt
//
LOCUS       DAORYL010000123         5718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_9662_length_5718_cov_4.291895, whole genome shotgun sequence.
ACCESSION   DAORYL010000123 DAORYL010000000
VERSION     DAORYL010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9662_length_5718_cov_4.291895"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..130)
                     /locus_tag="PLV81_14335"
     CDS             complement(<1..130)
                     /locus_tag="PLV81_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOM11316.1"
                     /translation="MENNYFEIGFIGGAINSAVGTVHKIASQMDGKFKLVAGCFSKD"
     gene            complement(164..1093)
                     /locus_tag="PLV81_14340"
     CDS             complement(164..1093)
                     /locus_tag="PLV81_14340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012856104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOM11317.1"
                     /translation="MKQPYFSVVIPVYKCPWTLYELYQRLVKTLSTINKNFEIIMVDD
                     GSPLNDWEVIKELAAKDKRVKGIKLSRNFGQHYAITAGLDFAKGRWVIVMDCDLQDQP
                     EEILKLYNKAMEGYDIVVGRRAQRKDGFFKRMSSRVFWKVYSYFTDTRIDGRISNFGI
                     YSYKVIENIKKFKEHTRSFGLFALWVGFKRIEIDIEHAERKKGKSTYTLKKLINLTID
                     SILSHSQKPLLLFVQLGILISIVSFLFAIFLIIQHFVLSIPVAGWTSIMVSLYFLSGI
                     IIACIGITGIYIGKVYNEVQNRPLYIIESATFD"
     gene            complement(1289..1852)
                     /locus_tag="PLV81_14345"
     CDS             complement(1289..1852)
                     /locus_tag="PLV81_14345"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOM11318.1"
                     /translation="MNNKHIIEHIREQFLNHGAQLPFTIISIVLFGSYAQEHHTEHSD
                     IDLLVIAEGINPVFHKRVKEIVTIKKIFSLGILLNIIIMTQNECIDNFCNHNPLFLDI
                     AVSGIVIVDTGCFLQSLIDETLHYINIHNLQNSIDGWKFPVNYQKETFLSSISNKDFA
                     YAMLADGKRNYTVNPDWKEKLFMNSLN"
     gene            complement(1845..2927)
                     /gene="rfbB"
                     /locus_tag="PLV81_14350"
     CDS             complement(1845..2927)
                     /gene="rfbB"
                     /locus_tag="PLV81_14350"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOM11319.1"
                     /translation="MKTILVTGGCGFIGSNFIPYLLSKYSDYAIINIDKLTYAADVTN
                     LKEVEHNSAYTFIQGDICNKELIQSIFETYSIKGVIHFAAESHVDNSIKQSYEFIQTN
                     VVGTHTLIDTAYRFWMEKPFVYKKEFEGCRFHHISTDEVYGSLGSTGYFTETTPYAPN
                     SPYSASKAASDMIVRAYFHTYGLNIVITNCSNNFGPKQHDEKLIPTIIRNALHGNPIP
                     IYGDGKNVRDWLYVLDHCRAIDVVYHNGRAGQTYNIGGRTEKKNIYVAQAICEILDRI
                     FPATSNSLIGNSKNTSIKSYKELITFVEDRPGHDRRYAIDPTKIETELNWKPVWNFEE
                     ALVKTVEWYINKYIGYQCYVIKEKYE"
     gene            complement(2928..>3330)
                     /locus_tag="PLV81_14355"
     CDS             complement(2928..>3330)
                     /locus_tag="PLV81_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOM11320.1"
                     /translation="IEEKPKAPKSNYAVIGLYFYPNDVVQKSKKITPSYRGELEITSV
                     NQLYLQENRLKVKIMGRGFAWLDTGTHESLIEASNFVKIIEDRTGLKIGCIEEIAFRN
                     GWITKDQLLKIAQSLQKTDYGNYLIRLAKGE"
     assembly_gap    3331..3340
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3341..3812)
                     /locus_tag="PLV81_14360"
     CDS             complement(<3341..3812)
                     /locus_tag="PLV81_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOM11321.1"
                     /translation="MKGIILAGGSGTRLYPITKAICKQLLPVYDKPLIYYPLSVLMLA
                     SIRDILIISTPNDINRFKELFGNGLDYGLNISYAVQEKPNGIAEAFIIGQDFIGNDDV
                     CLVLGDNIFFGHGLINLLQDTKTIVKAEHKAVIFGYYVHDPHRYGVVEFDDHFEV"
     gene            complement(3906..>5718)
                     /locus_tag="PLV81_14365"
     CDS             complement(3906..>5718)
                     /locus_tag="PLV81_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM11322.1"
                     /translation="AYKNNVLFHPISLLIILMGIISHGSSSGSMIKPELFTYVFMSIT
                     VFIWWMFILDKEKNYKLLYFLPLVILIWVNSHGGFIFAAPFFVCIIAGSLLNYKFSNN
                     IGFTPFHIKHIIIASALSLFATFITPYGYKYHLSLLSGLLLKPDIENLKTVSAYGPTL
                     VVPTYVLYFGIPIILLLYVFFSTKHFKKLDWVFVTTNIIYAILYTIFGRLMYYWIPVY
                     VFSILYYTKDDVLYYKPKKKWVPALIITLSLIVALDQVRWQTLINFTNSITWFGFGDS
                     YINSHEEAEFIATYYPGYKVGNPYNCGGYFLWRLYPQNKIMIDPRYFPFKSWYREYIK
                     FMTNQDVDKFLQKYDFDILHLQHMDLLLYPHFLTNPDWKLAFYGTGGMVFVKRHIPLP
                     DNRIQRSEYIFSYPSLHQSSLILSFALRIGDWETCERILQHMKSEFTWFNQIKFVEAA
                     ENLYMGTRAYFRRQYDTALTLYEKALGKVPVPPNILSAMYHYKAVDLWTEGKTYEALD
                     YIKKVLETKDDDPYGLYNLGVINWYIYKKQLESGVSFLDDNQARSIWLKPFEAFIQFA
                     KDKPGAKPALDIVNAIINGKYTGKPPLIYPPEPKQLQ"
BASE COUNT         1892 a         1013 c          904 g         1899 t
ORIGIN      
        1 gatctttact aaaacatcca gcaactagtt taaacttacc gtccatctgt gaagcgattt
       61 tatgtacagt tccaactgct gaattaatgg caccaccaat aaaaccaatt tcaaagtaat
      121 tattttccat ttcggcaatt tctccattaa aactattaaa ctattaatca aatgtagcag
      181 attctattat atataaaggt ctattttgga cttcattata tactttacct atgtaaatac
      241 ctgttatacc aatacatgca atgattatac ctgaaaggaa ataaagagat accattatac
      301 tagtccaacc agctactgga attgatagta caaaatgttg tataattaaa aaaatggcaa
      361 aaagaaatga aactattgaa ataagaattc ctaattgcac aaatagcaat aaaggttttt
      421 gtgaatgaga taatattgaa tctattgtca aattgattaa ttttttcaag gtataggttg
      481 atttaccttt tttcctttct gcatgttcta tgtcaatttc aatcctttta aaaccaaccc
      541 ataatgcaaa aagtccaaag gaacgtgtat gttctttaaa tttctttatg ttttctatta
      601 ccttatagga atatattcca aaattgctaa ttctgccatc aatcctcgta tcagtaaaat
      661 aactatacac cttccaaaag actctcgaac tcatcctctt aaaaaaccca tccttcctct
      721 gtgcacgcct tcctaccaca atgtcgtacc cttccattgc cttgttataa agctttagta
      781 tttcttcagg ctgatcctgc aaatcgcagt ccatcacaat tacccatctg ccttttgcaa
      841 aatcaaggcc agcagtaatg gcataatgct gtccaaaatt gcgtgaaagt ttgataccct
      901 ttacacgctt atcctttgca gcaagctctt ttattacttc ccagtcattt aaggggctgc
      961 catcatcaac catgatgatt tcaaaatttt tgtttatagt actcaacgtt ttaacaaggc
     1021 gctggtacag ttcatacaat gtccatgggc atttatatac aggaataaca actgaaaagt
     1081 atggctgttt catgtaaatc atcctatatc tataatatca ctcaataaac caatacatac
     1141 aaactcattt gatacatcaa gaactatttt tgctttgctg caaatatcaa tagcatcttg
     1201 atgggtataa tgttcatatg gtatccataa atccccttgt attactgaag ggtatcgtga
     1261 taaagataca tgaggctcaa tttctttact aattgagact attcataaat aatttttctt
     1321 tccaatctgg attaacagtg taattgcgtt tgccatcagc cagcattgca tatgcaaaat
     1381 ctttattgct tattgatgat aaaaatgtct ctttttgata attaacagga aatttccacc
     1441 catctatact attttgtaaa ttgtgtatat taatatagtg cagggtttca tcaattaaag
     1501 actgtaaaaa gcatccagta tcaacaataa caatcccgct aacagcaata tccagaaaga
     1561 gtgggttatg attgcaaaaa ttatcaatac attcattttg ggtcattata atgatattaa
     1621 gcagtatacc taaagaaaaa atttttttaa tggtaactat ctccttaaca cgcttatgaa
     1681 atactggatt aataccttca gctattacca gtaaatcaat atcagaatgt tctgtgtggt
     1741 gttcttgtgc ataagagcca aaaagcacaa tagatataat agtgaagggc agctgtgccc
     1801 catgattaag aaattgttca cgaatatgtt ctataatatg cttgttattc atacttttct
     1861 tttattacat agcattgata tccaatatat ttattaatat accattcaac agttttaacc
     1921 agagcctctt caaagttcca aacaggcttc cagttaagct cggtttcaat tttggttgga
     1981 tctatagcat aacgtctatc atgccctggc ctatcttcca caaaggtaat taattcttta
     2041 tatgatttaa tagaagtgtt ctttgaattt ccaattaagg aattactggt agctggaaat
     2101 attctgtcaa gtatttcgca tattgcttgt gccacataaa tatttttttt ttcagtacgc
     2161 ccaccaatgt tatacgtttg tccagctctg ccattatgat acacaacatc aatagccctg
     2221 caatgatcaa gtacgtagag ccagtcacgg acatttttgc catcaccata aatgggaata
     2281 ggattgccat gcaaagcatt acggataatg gttgggatga gcttttcatc atgttgtttt
     2341 ggcccaaaat tatttgagca gttagtgata acaatgttta gtccatatgt atgaaagtaa
     2401 gcacggacaa tcatgtcgct tgcagctttt gaagcgctat aggggctatt aggtgcatac
     2461 ggagttgttt cagtaaaata gccagtacta ccaagggaac cataaacctc atcagttgaa
     2521 atgtggtgaa agcggcaacc ttcaaattct tttttatata caaatggctt ttccatccag
     2581 aagcggtaag cggtgtcaat aagcgtatga gtaccaacaa catttgtttg aataaattca
     2641 tacgattgtt taatagaatt atctacatgg ctttcagcag caaagtgtat aacgccctta
     2701 attgaatagg tttcaaaaat agattgtatt aattccttat tgcaaatatc gccctggatg
     2761 aaggtgtatg cagaattatg ctcaacttct ttaagattag taacatctgc tgcatacgtc
     2821 agcttatcaa tgtttataat tgcataatca gaatattttg ataacaaata ggggataaaa
     2881 ttgcttccaa taaagccgca accgcctgta acaagaattg ttttcactta ttcaccttta
     2941 gctaatcgta tgagatagtt accataatcg gttttttgta atgattgggc tattttcagt
     3001 aactggtctt ttgttatcca tccgtttcta aatgcaattt cttcaatgca accaattttt
     3061 aaacctgttc tatcctcaat aatttttaca aaattactgg cttcaataag gctttcatgc
     3121 gttcccgtat caagccaggc aaagccacgt cccattatct taacttttaa gcgattttcc
     3181 tgtaaataaa gttgattgac actggttatc tctaactcac ctctatagct gggcgtaatt
     3241 ttttttgatt tttgaaccac atcgttggga taaaaataca ggccaataac agcataattg
     3301 cttttgggtg cttttggttt ttcctcaatg nnnnnnnnnn atacttcaaa atgatcatca
     3361 aattcaacaa ctccatatct atgggggtca tgcacatagt agccaaagat aacagcttta
     3421 tgttctgcct ttacaatagt tttggtgtct tgaagcaggt taattagtcc gtgtccaaaa
     3481 aagatattat cacccagtac taaacataca tcatcattgc caatgaaatc ctgaccaatg
     3541 ataaaagctt cagcaatgcc attgggtttt tcctgaacag cataagaaat atttaatcca
     3601 taatccagtc cattgccaaa taactcttta aaacggttga tgtcattggg ggtggaaata
     3661 attaaaatat cacgtataga cgcaagcatc agtacagaaa gtggataata tatcaatggc
     3721 ttatcataca ctggtaacag ttgcttgcat atagcctttg tgattggata taagcgtgta
     3781 cctgagcctc ctgctaaaat tattcctttc ataataaccc aattttagtg taaaataaat
     3841 taaaaaatta attttgttca cataaaatat gaatcaaatc ttgaagcgtc aacaataatt
     3901 ttgtattact gcaattgttt gggctcaggt ggatagatga gtggtggttt gccagtatat
     3961 ttcccattga tgatagcatt gacaatgtct aaagcgggtt ttgcacctgg tttgtctttg
     4021 gcaaattgta taaaagcttc aaatggctta agccagatag agcgtgcctg attgtcatct
     4081 aaaaaagaaa cacccgattc aagttgcttt ttataaatat accagtttat tactcctaaa
     4141 ttataaagac catacggatc atcgtccttt gtttccagta ccttcttgat ataatcaagt
     4201 gcttcatatg ttttaccttc agtccataaa tcaactgctt tataatgata catagcagat
     4261 aaaatgtttg gtggtacagg aactttgccc agagcttttt catatagtgt aagtgctgta
     4321 tcgtattgtc ttctaaaata tgcacgagtt cccatataaa gattttctgc tgcttcaaca
     4381 aattttattt gattaaacca tgtaaattca cttttcatat gttgcaatat tctttcgcat
     4441 gtttcccagt cgccaattct tagtgcaaaa cttaaaatta atgaagattg atgtaagctg
     4501 ggataagaaa aaatatactc gcttcgttga attctattgt cgggtaatgg tatatgtcgt
     4561 ttaacaaaaa ccattccacc cgtaccataa aaagctaatt tccaatcagg gttggtcaaa
     4621 aaatgtgggt aaagcaataa atccatatgt tgcaggtgca atatgtcaaa atcatacttt
     4681 tgtaaaaatt tatccacatc ctgattagtc ataaacttaa tgtattctct ataccagctt
     4741 ttaaatggaa aatatcgtgg gtctatcatg attttgtttt gtgggtataa acgccataag
     4801 aaataaccac cacagttgta agggttgcca actttgtaac cagggtaata cgtagcaatg
     4861 aattctgctt cctcatgtga gttaatgtaa ctatcgccaa aaccaaacca ggtaatgcta
     4921 tttgtaaaat ttatgagtgt ttgccagcgt acctgatcaa gcgctacaat aagcgacaga
     4981 gtaataataa gcgcaggaac ccatttcttt tttggcttat aataaagaac atcatctttt
     5041 gtataatata aaatgctaaa tacataaact ggtatccagt aatacattaa ccgtccaaag
     5101 attgtataaa ggattgcata tattatattt gtggttacaa acacccaatc tagcttttta
     5161 aaatgttttg tactgaaaaa aacatataaa agtaaaatta ttggtatgcc aaaatacagt
     5221 acgtatgtag gaactaccag ggtggggcca taagcgctga ctgtttttaa gttttcaata
     5281 tcgggtttta ataaaagtcc tgataataag ctaagatgat atttgtaccc atacggggtg
     5341 ataaaggtag caaatagaga tagagctgaa gcaatgataa tatgtttaat gtgaaaaggg
     5401 gtaaatccta tattgttact aaatttatag tttaacaaac ttccagctat aatgcataca
     5461 aaaaacggtg cagcaaagat aaatccacca tgactgttaa cccatattag aataactaag
     5521 ggaagaaaat ataatagttt gtagtttttt tctttatcaa gtataaacat ccaccagata
     5581 aatactgtta tactcataaa aacgtaagta aacaattcag gttttatcat actaccactg
     5641 gaagatccat gtgaaatgat ccccattaag attataagca ggcttatagg atgaaacaat
     5701 acgttgtttt tataggca
//
LOCUS       DAORYL010000124         5631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_9865_length_5631_cov_13.365136, whole genome shotgun sequence.
ACCESSION   DAORYL010000124 DAORYL010000000
VERSION     DAORYL010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9865_length_5631_cov_13.365136"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(375..1028)
                     /locus_tag="PLV81_14370"
     CDS             complement(375..1028)
                     /locus_tag="PLV81_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOM11323.1"
                     /translation="MKRNPVSGQTKREEIANVITHSIGIALGIAGLAILVVFASIKGD
                     PWKIVSFSIYGASIIILYSASTIYHSIKNVTLKKIFQIVDHSSIFILIAGTYTPFVLV
                     NMRGPWGWTLFGLVWGLAISGIIIKSFLGTGGDKLSTVVYLAMGWIIVIAFKKMITSI
                     PLMGIVWLVIGGLCYSLGAIVFLLENNMPFNHPVWHLFVLAGTVTHFFGILFYVLPY"
     gene            complement(1035..1601)
                     /locus_tag="PLV81_14375"
     CDS             complement(1035..1601)
                     /locus_tag="PLV81_14375"
                     /inference="COORDINATES: protein motif:HMM:NF021487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOM11324.1"
                     /translation="MFKEEIKRICKALNKNKIPYMIVGGQAVLLYGEPRLTRDIDITL
                     GIDIDRLDDVLNIVKKINLKILTPDVKNFVKETMVLPVLGSRGIRIDMIFSFTEYERN
                     AIQRAKKILTDDVEISYASPEDVIIQKIFAGRERDIEDAKIIIAKQTVDDAYIMHWLK
                     EFGKVLDKNFVDIYRKLKKQKKHIPDNK"
     gene            complement(1583..1789)
                     /locus_tag="PLV81_14380"
     CDS             complement(1583..1789)
                     /locus_tag="PLV81_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11325.1"
                     /translation="MIKNAKLLEEFEKECIIKHPLSYDEALALLDGMWELGISLGVLP
                     PEDPLEGIDVDIKVARILNCLKKK"
     gene            complement(1786..2916)
                     /locus_tag="PLV81_14385"
     CDS             complement(1786..2916)
                     /locus_tag="PLV81_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOM11326.1"
                     /translation="MASIEVKEIDITNKKMMKQFIMLPWTTKIYENDPAWVPPIIADQ
                     KKLFNPKTGYFYEIGEVAYFLAYKDGVPVGRITAHINRMYEEKYNKETGFFGFYEAID
                     DLDVAKALFAAAEGWLKKKGKTRVEGPQSFSIYDSVGFEVLGEDIMPVVGLFHFAPYY
                     KHHAEACGYTKCIDWHCFLVRKIDDFKPYLKDVRENIMKTQDVKYIYYSPKELKKRAK
                     EIHEIFNRAWDGNWGHLDLTQKQFDMLVDELKLVAVPELIVFAEKDGKTVGFIVSLPD
                     MNPALKILNGHLYPWRLIKALRSIKKVKKIRTIIMGVLPEYRGQKIDDVFYLYTIENG
                     IRLGYTESDCSLIVETNYKMIGALKPLKGQIYKTYRIYEKKI"
     gene            complement(3059..3766)
                     /locus_tag="PLV81_14390"
     CDS             complement(3059..3766)
                     /locus_tag="PLV81_14390"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOM11327.1"
                     /translation="MAQNKAQFLEDLRNNTIGNCTRCKLHATRSTIVFGEGNPEASVM
                     FIGEGPGKDEDAQGRPFVGKAGQLLTAVIEKGMKLKRSDVYIANVVKCRPTVNLELKR
                     DRPPDDEEVAACGPFLKKQIEIIQPKVIVTLGNPSTKFLVNPKEGITKVHGVWHLYNN
                     IPVMPTFHPSYILRNGGDSSPLKRLVWEDIKKVIAYLHGEITFEAISGKSEVTLDISI
                     NVAGDEKRDDEVQGSLF"
     gene            complement(3766..4773)
                     /locus_tag="PLV81_14395"
     CDS             complement(3766..4773)
                     /locus_tag="PLV81_14395"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOM11328.1"
                     /translation="MFNPFKIFKNTKVGLALGSGGAKGVSHIAVLDYCTKKEIHVSMI
                     AGSSIGAVIGSLYAYGALPEFTSFLKSIKSNDLFAYFDPVFPKTGLVQGRRAMELLAK
                     FIPKSVDIEDLKLPVAIVATDFYSGKPVVFTKGNVLDAIRASISIPGIFVPVKYMDTV
                     LIDGGVSNPLPVDVVKQMGAGITIAVNLHPTIKIKRAIAPAIAKPHQESTNENSSNDI
                     IEHVDTESLDTTTVVTQTKGWLTQVKKWLGIKIESYKKIPEDTMPNIFEIVAGTIDIM
                     EYMNTTLMITYNKPDVLIEPDLVHVGTLDFTKVDFLLNEGYAACLRVEKDIKRKILRW
                     I"
     gene            5212..>5631
                     /locus_tag="PLV81_14400"
     CDS             5212..>5631
                     /locus_tag="PLV81_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOM11329.1"
                     /translation="MALRRVISLSASEDRLEKLIQERLQPGINKEEIDRRIWDLFGEV
                     WCVMFTDLAGFSRRVATFGIIHFLQIIYESQRLLVPIIEDYDGILLKTEGDSMLVIFR
                     NVAKGLECCKKMHQVLKDYNKSKPEEDHIVLCIGLGYG"
BASE COUNT         1769 a         1145 c          868 g         1849 t
ORIGIN      
        1 tgcaagcaaa ccatacatgt cttcaatgga tttgcaaaca ggaatgattt ttatttcact
       61 cttttctaac ctgaaataca atgtatctcc agttttaaga ttgagattat ctcttacaac
      121 tttaggaatt gtaatgtgtc cttgacctgt caaagttgaa taaggcataa aatccccacg
      181 taatggtttt tccttactta aggcaaggaa aattttagca ctttcaatta tattttcact
      241 aagtctttaa attttctcaa tttattcaca tactactttt tgttaaaaga aaatagaatt
      301 gtgcatactg caactatttc agaagcaatt attaaatcaa tttaatacac atattttatt
      361 atttattact tttactaata tggcaacaca tagaaaagta ttccaaagaa atgagtgacc
      421 gtaccagcaa gaacaaaaag atgccacaca ggatgattaa acggcatatt attttccagt
      481 aaaaaaacta ttgcacccaa tgaatagcat aatcccccaa taacaagcca cactattccc
      541 atcaaaggga tagatgtaat catcttttta aatgcaataa caataatcca tcccattgcc
      601 aagtatacca cggtagaaag tttatcccca ccagtaccta aaaaagattt tataataatc
      661 cctgaaattg caagccccca taccagacca aacagcgtcc acccccaggg accgcgcata
      721 ttaaccagca caaatggcgt ataggtacct gcaataagaa taaaaattga tgaatggtca
      781 actatctgaa atattttctt taacgttaca ttttttattg aatggtagat agtcgaagcg
      841 ctataaagaa taattattga agctccataa attgaaaatg acacgatctt ccagggatca
      901 ccttttattg aagcaaaaac cacaaggatt gcaaggcctg caatacctaa agcaatccca
      961 atactgtggg ttataacatt tgcaatttct tcacgtttag tctgtccgct tacaggattt
     1021 cgtttcatac catattattt attatcaggt atatgttttt tttgtttttt taatttcctg
     1081 taaatatcaa caaaattctt atcgagtacc tttccaaact cttttaacca atgcataata
     1141 taggcatcat ctaccgtttg ctttgcaatt ataatttttg catcctcaat atccctttct
     1201 cttccagcaa atattttttg aattattaca tcttcagggg atgcataact aatttctaca
     1261 tcgtctgtca atattttctt tgctctttgt attgcatttc tttcatattc ggtaaatgaa
     1321 aatatcatat caattcttat gcctcttgat ccaagtacag gtaataccat agtttctttc
     1381 acaaaatttt taacatcagg cgtcaatatt tttaaattta ttttttttac aatattcaag
     1441 acatcatcca atctatctat gtcaatacct aatgtaatat caatatccct ggtaagcctt
     1501 ggctcaccat acagcaatac agcctggcca cctactatca tgtatggaat tttattttta
     1561 tttaatgctt tgcaaattct ttttatttct tctttaaaca attcagtatc cttgctactt
     1621 taatatcaac atcaatcccc tctaacgggt cttcaggtgg cagcacccct aagcttatgc
     1681 ctaactccca cataccatca agtaatgcaa gtgcctcatc atagcttaag gggtgcttta
     1741 ttatacattc cttttcaaat tcctctaaca gttttgcatt cttaatcata tcttcttttc
     1801 ataaattcga tacgtcttgt aaatttgtcc tttaagcggt tttaaggcac ctatcatttt
     1861 gtaattagtt tccactatca gagagcagtc tgattcggta taacccaacc gtatgccatt
     1921 ttcaatggta tacagataga atacatcatc aattttctgc cccctatatt caggcaacac
     1981 acccatgatg atagtacgaa tctttttaac ttttttgatt gaacgcaacg ctttaatcaa
     2041 acgccatgga taaaggtgac catttaaaat cttgagtgct ggattcatat caggtaggga
     2101 tacaataaac ccaactgttt tgccatcctt ttctgcaaac acaataagtt ctggcacagc
     2161 aacaagcttc aactcatcaa caagcatatc aaactgcttc tgcgttaaat caagatgccc
     2221 ccagttacca tcccatgctc tattaaatat ttcatgaatc tctttagcac gctttttcaa
     2281 ttctttaggg ctgtagtata tatattttac atcctgcgtc ttcatgatgt tttcacgaac
     2341 atcttttaaa tacggcttga aatcatcaat ctttcttacc agaaagcaat gccagtcaat
     2401 acatttggta tagccacacg cttctgcatg atgtttgtaa tacggagcaa aatggaatag
     2461 ccccacaaca ggcatgatat cctcacccag cacttcaaag ccaaccgagt cataaatgga
     2521 aaaactttga ggaccttcaa cacgtgtttt tccttttttc tttaaccacc cttcagcagc
     2581 agcaaacaaa gcctttgcaa catccaggtc atcaatggct tcataaaatc caaaaaaacc
     2641 tgtttcttta ttgtattttt cttcatacat cctgttgatg tgagcggtga ttctgccaac
     2701 aggcacacca tccttatacg ctaaaaaata tgcaacttca ccaatttcgt aaaagtaacc
     2761 agtctttgga ttaaagagct ttttttgatc agcaatgatg ggtggcaccc acgccgggtc
     2821 attttcatag atctttgtag tccacggaag catgatgaac tgcttcatca tctttttgtt
     2881 tgtaatgtca atttctttta cctctattga tgccataaat gtacccccac aaaagtttaa
     2941 aattctaaag agtatatata aactaattga tgaaaccgta acctggtagc atcaattaaa
     3001 aaaacattga atgggcgata gttaccgcta tccatacctg atactatata caaacatgtt
     3061 aaaacaaact accctgaacc tcatcatcac gtttctcatc accagcaacg ttaatactga
     3121 tatctaaggt tacctctgat tttccagaaa tagcttcaaa cgtgatttca ccatgtaaat
     3181 atgcgataac ctttttgatg tcttcccaca caagtcgttt gagtgggctg ctatcaccac
     3241 catttcgtaa aatatacgaa ggatgaaacg ttggcataac agggatattg ttatacaggt
     3301 gccacacacc gtgcactttt gtaatgcctt cctttggatt aaccaaaaat tttgtagaag
     3361 ggttccctaa cgtaacgatt acttttggct gaataatttc aatttgcttt tttaaaaacg
     3421 gcccgcaggc tgctacttct tcatcatcgg gtggacggtc acgctttaac tctaagttga
     3481 ccgttggcct gcatttaact acattggcaa tatacacatc gctgcgctta agcttcatcc
     3541 ctttttcaat gacagcggtc aaaagctgtc ccgctttccc cacaaatggt ctgccctggg
     3601 cgtcttcatc cttgcctgga ccttcgccaa tgaacataac actggcttca ggattacctt
     3661 caccaaaaac aatggtactg cgcgttgcgt gaagtttaca gcgtgtgcaa ttcccaatag
     3721 tattattgcg taaatcctct aagaactgtg ccttgttttg tgccatcata tccaccgcaa
     3781 tatcttgcgt tttatatcct tctcaactct tagacaggct gcataacctt catttaaaag
     3841 aaaatcaact tttgtaaaat ccagtgtccc tacatggaca aggtcaggtt caatgagtac
     3901 atcaggctta ttatatgtaa tcatgagcgt tgtgttcatg tattccatga tgtcaattgt
     3961 cccggctact atctcaaaaa tattgggcat agtatcttca ggtatctttt tataactttc
     4021 aatctttatg ccaagccact ttttaacctg cgtcagccaa ccctttgtct gagtaactac
     4081 tgttgttgta tcaagtgatt ctgtatcaac gtgctcaata atatcatttg atgaattttc
     4141 attggtagac tcttggtgcg gttttgcaat tgcaggagct atcgcccgct taatcttaat
     4201 tgttggatgc agattaactg ctatggtaat gccagcaccc atctgcttaa ccacatcaac
     4261 tggtaacgga ttacttacac caccatctat aagcaccgta tccatatatt taaccggaac
     4321 aaaaattcct ggtattgaaa tacttgccct aatggcatca aggacattcc cttttgtaaa
     4381 aaccacaggc tttccgctat aaaagtcggt tgcaacaatg gcaacaggca gttttaaatc
     4441 ttcaatatca acacttttag gaataaattt tgcaagtaac tccattgcac gccggccctg
     4501 caccagtcct gtttttggaa acactggatc aaaatatgca aagagatcat tgctttttat
     4561 tgattttaaa aacgaagtga attcgggcaa tgcaccatat gcatacagcg aaccaatcac
     4621 agcaccaatg cttgatcctg caatcattga aacatgaatt tcttttttgg tacaataatc
     4681 aagcacagca atatgtgaca cacctttagc accaccgcta ccaagtgcaa ggcctacttt
     4741 tgtgttttta aatattttaa atggattaaa catatttttt tgataataca aaagattaac
     4801 tgatgaaaaa gtaaattttt tattttgtca agaggatttt agtaatttat aaacctgaat
     4861 gtaattcaat catgacaata ccaatatatt taatcaaata taatagtgcc gccacttcag
     4921 attctggtgc aggataaaca tttaactctg taatactgtt agatatagag tgcactgttg
     4981 tgtatatgat tattataata ttatttatat caggaaagcg gggtggaatt caacaggtgg
     5041 ttataagatt taatattata taaaatttat tataaagtat aacataccat gtgggatgtc
     5101 aatatctttg cattaactat tgatttattt acattcacaa cacgtatatc tattgacatt
     5161 aacatagaat tactttacat ttaatgccaa tatatttgcg gggggaaaat catggcacta
     5221 agaagagtaa tttcacttag cgcatcagaa gaccgtcttg aaaaacttat tcaggaacgt
     5281 ttgcagccag gtattaataa agaagaaatt gataggcgaa tctgggatct ttttggagag
     5341 gtgtggtgtg tgatgtttac tgatctggca ggcttttcaa gaagagttgc tacatttggt
     5401 ataattcact ttttacagat tatatacgaa tcacagcgct tacttgttcc tataatcgaa
     5461 gattatgatg ggatactatt aaaaactgaa ggcgacagta tgctggtaat attcagaaat
     5521 gttgcaaaag gtctggaatg ttgcaaaaaa atgcatcagg tattgaagga ttataacaaa
     5581 agtaaacctg aagaagatca tattgtatta tgtattggtc tgggatatgg g
//
LOCUS       DAORYL010000125         5496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_10128_length_5496_cov_8.262819, whole genome shotgun sequence.
ACCESSION   DAORYL010000125 DAORYL010000000
VERSION     DAORYL010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10128_length_5496_cov_8.262819"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            880..1059
                     /locus_tag="PLV81_14405"
     CDS             880..1059
                     /locus_tag="PLV81_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11330.1"
                     /translation="MLEYNVKNEYELIAKHCPRCQALVMAAVVKTLQGEILKYKDIRC
                     SSCDWQSPLAQLKSS"
     gene            1204..1710
                     /locus_tag="PLV81_14410"
     CDS             1204..1710
                     /locus_tag="PLV81_14410"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOM11331.1"
                     /translation="MAVEQTLIIIKPDGLVKSLVGNILTRLSEAKLIIVGAKALRPTR
                     ELAEKHYEHLKDKPFFEELIKYIMGELHKTYRVVAFVYEGEDAIKKVRDIVGDTNPEN
                     ANPVTIRGAYGRITTSGVFENVVHASSSPEDAEKEIKLWFEPHELVDIIYPVKEVTIE
                     KITKTVWA"
     gene            1968..2615
                     /locus_tag="PLV81_14415"
     CDS             1968..2615
                     /locus_tag="PLV81_14415"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF011100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOM11332.1"
                     /translation="MRMILLGAPGAGKGTIAKMLVEKYGIVQISTGDILRQAVKEGTP
                     VGKKAAEYMNTGKLVPDDLIMDIIEERIQRPDCAKGFIFDGFPRTVPQAEALEKLLSA
                     KNMTIDAIINLEVPEDVVLKRLASRRTCSNPGCQAIYNIHTMPPRKEGVCDKCGSPLV
                     QRDDETEEAIRVRLATYYEKTQPLIDYYKNNTAYFAVPSLVAQETFDEIVKRLGK"
     gene            2747..2835
                     /locus_tag="PLV81_14420"
     tRNA            2747..2835
                     /locus_tag="PLV81_14420"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2781..2783,aa:Ser,seq:cga)
     gene            2959..3417
                     /locus_tag="PLV81_14425"
     CDS             2959..3417
                     /locus_tag="PLV81_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11333.1"
                     /translation="MRKFKSVLIVLFCIANSITYSNAKDISYTLDDRDRLIRVEEGLK
                     GVNQRIDSIEKRIDGLDKRIDGLQGLMYVVIGAIIAQTLAVVGFSLWDRRSALMPVTR
                     KTKELEEYIESTLKETKEIKEREIVLENAIREYAKHEPKLYEVLKSLRLL"
     gene            3449..4732
                     /locus_tag="PLV81_14430"
     CDS             3449..4732
                     /locus_tag="PLV81_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOM11334.1"
                     /translation="MMTKHTIIIGDSRYMAEVDDECCHLIVTSPPYWQLKDYGSDNQI
                     GFNDTYEEYINNLNLVWKECHRVLYPGCRLCINIGDQFARSVYYGRYKVIPIRTEIIK
                     FCETIGFDYMGAIIWQKPTTMNTTGGASVMGSFPYPRNGIIKIDYEFILIFKKWGDPP
                     KVTKEIKEQSKLSKEEWNEYFSGHWNFNGEKQDKHIAMFPEELPKRLIKMFSFVGDTI
                     LDPFLGSGTTTLASKNLNRNSIGYEINEKFIDLIKAKIGYDENLLINDTHISIVKQEY
                     QNRDWRKEWENLPYIFCDPIKFDKKIDPKKFTFGSKIDFTNKVEEKYYYVKKILSPEL
                     LLLNNDTIIKLIGVRTKEEKKQEAIEWLEKKLKNVRIYLKFDVVKYDDHNHLLCYIYL
                     KNKTFINMHLIKQGLAEVDETSNYRMKEKFVKIAG"
     gene            4736..5368
                     /locus_tag="PLV81_14435"
     CDS             4736..5368
                     /locus_tag="PLV81_14435"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MjaI family restriction endonuclease"
                     /protein_id="HOM11335.1"
                     /translation="MPKEWILNSAINRWGLQKKKWVGAVSDEIRKCSPNSIAEWEQYY
                     YSKVYPKEHLIEIGKKLYVKISEVMRAEIDDITEEDCIQYVINLVINRTFDGYITEKK
                     TIYEQLQHILNVKIEPAPDEWDRLYNVDFFIKIQDKFIGLQIKPAGYAFIPQIIQEKI
                     NQEKTHKKFEQKYGGRVFYIISVKEGDKKIIYNNEVIDAIKAEIIRLSGK"
BASE COUNT         2024 a          703 c         1142 g         1627 t
ORIGIN      
        1 tggctggtca tcgagcctgt cgagatgaag tcccttttcc ccttgtgaaa agaatgaagt
       61 ggagttgttg ccgtctagac aaaagaaagt gcaagggaag gctgtatata gcttcactgg
      121 atgcagacac cgttatgcgg ggggatagag atagttagtg agatgcctgg aaagattgta
      181 aaaaccatac catttgctaa tgggactgaa tgtgcctatt acaggaaatg agatgaaata
      241 ctggattgta aaatttattg tgcagaaccg tattgtgcat gttaacgaga ttgtggaaaa
      301 aaccaccagg gtataccgat attttttgcc agagaaaaaa ttttgaacat gtaagcaatg
      361 aagaaataga tagtataaca atggtgatac atacaccccg ggaaaatcta taaggatgga
      421 aaactttgcg tgaggtttta caggtaaaac tacatggtac actgcaaacg tgaaattgca
      481 aagtaatact ttaaaaaata aaaaaatata ttgacaaatt tatacttgta tataatgagt
      541 gagtaatcac tcattatggt taatttacaa ttatatgccg ataatagtat tgtgagtgaa
      601 attagcatta ttgcagttaa ggatgttagt gtattaattt taaggtagtt ccttgtaccg
      661 tgattatggg cagtagaaat gatggaaagg ggatgattgg gaatcttgtc cctgtttgga
      721 aagggataag gaactcaatt tttaaaggag gtacgtaata gagcacattt tatcgaaaac
      781 gataaaatgc ttgacatctt tgaaatttac ggtatattgc atttgagata taaaaacaaa
      841 atattgggat ttttgttata aataaacgga ggcgtatcca tgctggagta caatgttaag
      901 aatgagtatg agctcattgc caagcattgc ccacggtgcc aggctctggt tatggcagcg
      961 gtagttaaaa ccttacaggg tgaaatcctg aaatataagg atatccgttg ctcaagctgt
     1021 gattggcaaa gcccgctggc tcagctaaaa agttcttgaa aaatattggc tattcagagt
     1081 aatagcactg ccatagttaa ggcatcttac tgtggtcaat aacttgacag caagttagtt
     1141 attgtgttat tatcgcacag gagatgggat ccttaaaaaa atatactgat atgaggtagg
     1201 actatggcag tagagcaaac cttaataatt ataaagccag atgggcttgt taagtcttta
     1261 gtaggaaata ttttgacccg cctttcagaa gcaaaattaa taattgttgg tgcaaaagct
     1321 ttacgcccca ccagggagct tgctgagaag cattatgagc atttaaaaga taagccgttt
     1381 tttgaagagt tgattaagta cattatgggg gaattacata aaacctatag agttgttgcg
     1441 tttgtttatg aaggtgaaga tgcaataaag aaagtgcgcg acattgttgg tgatacaaac
     1501 cctgaaaatg caaatcctgt taccatacgg ggagcatacg ggcgaataac aacctctggt
     1561 gtatttgaaa atgttgtgca tgcatcatca agccctgaag atgccgaaaa ggaaattaag
     1621 ttgtggtttg agccacatga acttgttgat atcatttatc cagttaagga agtcacaatt
     1681 gaaaaaataa caaagacggt gtgggcgtaa ggttaaaagt ttacggtata tcatccatag
     1741 atgtgttgat agatgtagtg atgtaaactt accatcgctc ataaaaagta tatggtatca
     1801 ctataagttc aaaagaaaag agcagagatt atctctgatg attgccaatg gctgcgtggg
     1861 agcataccag gcaataaatg tctctcatgt ggccattctt caatgttggt aaatgattat
     1921 atacactgtc aggagatctt atattttttt tagaggggaa aaatagtatg cgaatgatat
     1981 tgttaggtgc accgggtgct ggtaaaggaa ctatcgctaa aatgcttgtt gaaaaatatg
     2041 gcatagttca gatttccacc ggcgacatat tgcggcaggc agttaaagaa ggaacaccgg
     2101 tagggaaaaa agcagcagag tacatgaata ctggaaaact ggttcccgat gacttgatca
     2161 tggatatcat agaagaacgc attcaacgac ctgactgcgc aaaaggtttt atatttgatg
     2221 gatttcccag aaccgtacct caagctgaag cacttgaaaa actgttgtct gcaaaaaata
     2281 tgaccattga cgccatcatt aatcttgaag tgcctgagga tgtggtatta aaaagacttg
     2341 ccagccgaag aacctgttct aatcccgggt gtcaggcaat ttataatatc cataccatgc
     2401 cacccaggaa ggaaggtgtt tgtgataaat gcggtagtcc tcttgtacag agagatgatg
     2461 aaaccgaaga agctattaga gtgcgccttg caacctatta tgaaaagaca caaccactga
     2521 ttgattatta taagaataat acagcatatt ttgctgttcc ttcactggtt gctcaagaaa
     2581 catttgatga aattgtaaaa cgattgggca aataaaaatt tctttacaat atataacaaa
     2641 tataattgtt ataaaaattg tttgggatat ttgtaataat atgtgtatga aatatattat
     2701 tgtagaagca cattcaaaat gtgcccctac aatataaaca atgtatggag agatgcctga
     2761 gaggttgaaa ggggcggtct cgaaaaccgt tgtcccgggt gactgggacc gagggttcga
     2821 atccctctct ctccgaatgg aatttgttct ataacgaaaa ttaattgaca gtatataaca
     2881 aatagtatat tataggcatt agcattcctg gtatgagaag aaatatttcc cagtggttaa
     2941 ataaggaaag gattaattat gagaaaattc aaaagtgtat taattgtatt gttttgtatt
     3001 gctaatagta taacttattc caatgctaag gatatttctt atactctgga tgatagagat
     3061 aggctgataa gagttgaaga agggttgaaa ggtgttaatc aacgtattga cagtatagaa
     3121 aagaggatag atggtttgga taaaagaatt gatggtttgc agggattaat gtatgtcgtt
     3181 attggtgcta tcattgcaca aactttagcg gtggttggtt tttccttatg ggatagaagg
     3241 tcagcactta tgcctgtaac aagaaaaaca aaagaacttg aagaatatat agaatctact
     3301 ctgaaagaaa caaaagaaat aaaagaacgt gaaattgtgc ttgaaaatgc tatacgtgaa
     3361 tatgcaaaac atgaaccaaa actgtacgag gtactgaaat cgttacgatt attgtaaaaa
     3421 ttttattttt attaagaaat aataagttat gatgacaaaa catacaataa ttattggtga
     3481 ttcccgatat atggcagagg tggatgatga atgctgccat ttaattgtta cttcaccacc
     3541 atattggcaa ttaaaagatt atggaagtga taatcaaatt gggtttaatg acacctatga
     3601 agaatatatc aataatctaa atttagtatg gaaggaatgc catagagttt tatatcctgg
     3661 atgtcgctta tgtataaata ttggagatca gtttgcacga tccgtttatt atggaagata
     3721 taaagtgatc cctatccgaa cagagataat aaaattttgt gaaaccatag gatttgatta
     3781 tatgggggca attatatggc aaaagcctac tacaatgaat accacgggtg gtgcatcggt
     3841 aatgggtagt ttcccctatc cacgtaatgg aattataaaa attgattatg aatttatatt
     3901 aatttttaaa aaatggggtg atcctccaaa agtcacaaaa gaaatcaaag aacaatctaa
     3961 attgagtaaa gaagaatgga atgagtattt ttcaggacac tggaatttta atggtgaaaa
     4021 acaagataaa catattgcaa tgtttcctga agaattacca aaacggctta ttaagatgtt
     4081 tagttttgta ggtgatacaa tacttgatcc attcttaggt agtggtacaa caacattagc
     4141 ttcaaaaaat cttaacagaa attctattgg ttatgaaata aatgaaaaat tcattgattt
     4201 aataaaagca aaaataggtt atgatgaaaa tttgcttatt aacgatacac atatttctat
     4261 agttaaacag gaatatcaaa atagagactg gagaaaagaa tgggaaaatt tgccctatat
     4321 tttttgtgat ccaataaagt ttgataaaaa aatagatcca aagaagttta catttggttc
     4381 taaaattgat tttactaata aagtagaaga aaaatattat tatgttaaaa agattctaag
     4441 tcctgagttg ttattattga ataatgatac aataattaaa cttataggtg taagaacaaa
     4501 agaagaaaaa aaacaagaag ctatagaatg gcttgaaaaa aaattaaaaa atgtaaggat
     4561 ttatttaaag tttgatgttg ttaaatatga tgaccataat catctacttt gttatattta
     4621 tttaaaaaat aaaacattta tcaatatgca tttgataaaa caaggattgg ctgaagttga
     4681 tgaaacaagt aattatagaa tgaaagaaaa gtttgttaag atagctgggt aactaatgcc
     4741 taaagagtgg attttaaata gtgcaattaa tagatggggc ttgcaaaaga aaaagtgggt
     4801 tggagctgta tctgatgaaa taagaaagtg ttctcctaat tctattgcag aatgggaaca
     4861 atattattat tcaaaggtgt atcctaagga acatcttatt gaaattggta aaaagttgta
     4921 tgtaaaaatt tctgaagtaa tgcgtgcaga aattgatgat ataactgaag aagattgcat
     4981 acagtatgtt ataaatttag taatcaatag aacttttgat ggatatataa ctgaaaagaa
     5041 aactatttat gaacaactac aacatatttt aaatgtaaaa attgaaccag cacctgatga
     5101 atgggataga ttgtacaatg ttgatttttt tattaaaata caggataaat tcattggttt
     5161 acaaattaaa cctgcgggct atgcttttat acctcaaata attcaagaaa aaataaatca
     5221 agagaagaca cataaaaaat ttgagcagaa atatggtgga agggtttttt atattatatc
     5281 agttaaagaa ggcgataaaa aaataattta taacaatgaa gtaattgatg ctattaaagc
     5341 tgaaatcata agactaagtg gtaaataaaa tacttataat caaactttat tgacaataaa
     5401 agtgtaggta gcaagaatgt agagatgtaa aaactaaaag accaggagag atgtctgaga
     5461 ggccgaaaga gcacggttgg aaaccgtgtg tagcgc
//
LOCUS       DAORYL010000126         5369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_10438_length_5369_cov_14.141513, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000126 DAORYL010000000
VERSION     DAORYL010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10438_length_5369_cov_14.141513"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..886)
                     /locus_tag="PLV81_14440"
     CDS             complement(257..886)
                     /locus_tag="PLV81_14440"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11336.1"
                     /translation="MSRNILVLQFATQLEAKPFIETLGLETLQQKPFLLYRGDSSLYA
                     IIGGIGVTSAAIAATYVYTTLNADKILNIGAAGALTPSLPLGTIGTVTTVFDTTRFDF
                     DTEKPFTYALTPMDTVIQLTCVSVTKPLRTSEERNQFAPTADIVDMELAGIARASQKF
                     SKLCYAIKYISDTPQHNTHEDIVQNIITLSQSTALEIVAYIQSLIAEYS"
     gene            complement(868..1656)
                     /locus_tag="PLV81_14445"
     CDS             complement(868..1656)
                     /locus_tag="PLV81_14445"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HOM11337.1"
                     /translation="MLSLAFSSCPNDTFIFHALVHHCINTYDLTFDVHIDDVESLNHA
                     ALQQQYDITKLSFHGYLHVQNYYDLLDAGAALGSGCGPLVVTRKGLTNLLNAKIATPG
                     QYTTAHLLFLLRFGNLCDITHVRFDEILPGINNGIFDAGVIIHEGRFIYHQYNCDMLI
                     DLGKWWEDVTGLPIPLGCIAIHKRHAHSKPLIEEIIRQSILYARENPDASKEYIRLLA
                     QELDDTVIQQHIDLYVNDFSLSLGTTGIKALQTLKEMAQCRGIF"
     gene            complement(1664..>1897)
                     /locus_tag="PLV81_14450"
     CDS             complement(1664..>1897)
                     /locus_tag="PLV81_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11338.1"
                     /translation="EDITDYFIQAQEDDYKEGAGLGLVLITMMLKGMGLTQSNFIIDS
                     DYEKTIASIIVPLTQATLQEYQKRISETTPKIA"
     assembly_gap    1898..1997
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2157..2561)
                     /locus_tag="PLV81_14455"
     CDS             complement(2157..2561)
                     /locus_tag="PLV81_14455"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOM11339.1"
                     /translation="MNKKDLASPCGIFCAACPRFYKYGICKGCRADTFHDACEIYDCC
                     VRIGGMEFCFECDLFPCQRLKTFSNYHPGENFAHYRHIVIDNLLKIKEIGVENWYVIM
                     NQKFMQGEYGIQQRNPDGSFDISCCPCCNTTK"
     gene            2834..3331
                     /locus_tag="PLV81_14460"
     CDS             2834..3331
                     /locus_tag="PLV81_14460"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HOM11340.1"
                     /translation="MKKRIPYVLAIITVLLGTYTIYSQIKWYDFNSGFAAVKNSKKPA
                     VIDFYADWCIWCKTMDKETFSNPAVEKKLSRDYIAIRINVEKNDSITFENKTYSAQEF
                     AAYCGVEGLPTLVFMDKEGKLVTRIPGYIKAEVFLSLLDYMKDECYKKKVSFHEYMQQ
                     KDCGK"
     gene            3342..3749
                     /locus_tag="PLV81_14465"
     CDS             3342..3749
                     /locus_tag="PLV81_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOM11341.1"
                     /translation="MEFIEWGEHLSVGVTVFDNEHKQLVSLVNKLNHALLAGSAKKTM
                     EEILQNLVNYTKIHFKHEEDYMVLYDYPEYEKHKKEHDALTDQVMDFYMRYQAGKTVF
                     SLELMNFLKDWLTNHILISDKKYKNFFQSKNVE"
     gene            complement(3750..5285)
                     /locus_tag="PLV81_14470"
     CDS             complement(3750..5285)
                     /locus_tag="PLV81_14470"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOM11342.1"
                     /translation="MTNNNTRKELFNDIIYNNHKRLFKAFTSIVILATLATYIIFLTG
                     TGSQYLTLIDIIVAFLSVSFILSIGFFIITRYRQKWFSPYLAIFSVMLSLFIFQYIIY
                     GSRELFAAHYIILGLSVFYFDTRVAIFSFITVIISQICLFILRPQLIPEGPVGSIIGV
                     RFLIYIWVGISAYFGTKATRALLDLALDKADEANNKHERIAVIAQNVEHSVGILNEKS
                     QEQASVVDTINDLANKQAASLEEISASVEELTSNAENISNTARSLYQEMQIANESVTD
                     LKKVFEEITKSAGTIAHAIETITSLSNDSIQKMDDTLKQFQDLITFSNNMSSFVEVIN
                     QIADQVNLLSLNASIEAARAGDAGRGFAVVADEISKLADATAQNASQIGKIIEQTQNL
                     MRSSNTSITDTSQFLSKLNEEIRTIMKEISHTNTLIQDVNVSIRAIANLNTQIYNAIE
                     TIETSTSEQRIANEEASKTIVYVSDAAQNLTDIVNTVSNVASAIKDIAQNLYTLVQAM
                     TKA"
BASE COUNT         1653 a          912 c         1057 g         1647 t
ORIGIN      
        1 taggatttct cctcactacg ttcgtcgaaa tgacagtgtg tgggaatagg atttctcctc
       61 actgtgttcg tcgaaatgac aaggtggaaa tggtttgtcg aaatgacagt gtgtgggatt
      121 aagatttctc ctcactgcgt tcgtcgaaat gacaaggcgg aaatggtttg tcgaaatgac
      181 agagggagaa taggatttct cctcactgcg ttcgtcgaaa tgacaaggtg agaatggttt
      241 gtcgaaatga gagttatcag gaatactcag caattaatga ttgtatatac gcaacaattt
      301 ccagtgccgt tgactgtgac agtgtgatga tgttctgtac aatatcttcg tgggtattgt
      361 gttgtggagt gtcgcttatg tattttattg cataacagag ttttgaaaat ttctggcttg
      421 cccgcgctat tcctgcaagt tccatatcta caatatctgc agtaggtgca aactggtttc
      481 gctcttcact ggtgcgcaac ggtttagtta cactcacgca ggttagctgt attacagtat
      541 ccattggggt cagagcatag gtaaaaggct tttcggtatc aaagtcaaat cgggtggtgt
      601 caaatactgt tgtaacagta cctattgttc ccagtggtaa gctcggcgtg agtgcacctg
      661 ctgctcctat attaagtatt ttgtcagcat tgagtgtggt gtacacgtag gtggcagcaa
      721 tggcagcgga agtgacccca atcccgccaa tgattgcata caatgagcta tcgcccctgt
      781 atagtaaaaa aggtttttgc tgtaacgtct caagccctaa cgtttcaata aaaggctttg
      841 cttccagttg tgtggcaaat tgtaacacta gaatattccg cgacattgtg ccatttcctt
      901 tagtgtttgc agcgctttga ttcctgtggt gcccagcgac aggctgaaat cattaacata
      961 aagatcaata tgctgctgta ttactgtatc atcaagctcc tgcgcaagca aacgaatata
     1021 ttcttttgac gcatcagggt tttccctggc atacagtatt gattggcgga tgatttcttc
     1081 aatcagtggt ttgctgtgtg catgtctttt gtgaatggca atacatccca gtggaatggg
     1141 taagccggtt acatcctccc accatttgcc taagtcaatc aacatgtcac aattatactg
     1201 gtgatagata aaacgtcctt cgtggataat aacgcctgca tcaaatatac cattattgat
     1261 gcctggcagg atttcatcaa agcgtacatg tgtaatgtca cacagattcc caaaccgtag
     1321 caaaaataaa agatgtgcag tggtgtattg ccccggtgtg gcaatttttg cattaagaag
     1381 attggtaagc cctttgcgtg ttaccactaa aggaccgcat ccagatccca gtgctgcacc
     1441 agcgtcaaga aggtcataat agttctgaac atgcagataa ccatgaaatg acagcttggt
     1501 tatatcatac tgctgttgta aggccgcatg atttaatgac tcaacatcat caatgtgtac
     1561 atcaaatgtt aaatcatagg tattaataca atgatggaca agtgcatgaa aaataaaggt
     1621 atcgttaggg catgatgaaa atgctaatga tagcatactg ttttcaggca atctttggag
     1681 ttgtttccga aatacgtttc tggtattcct gtaatgtggc ctgtgttaat ggtacaatga
     1741 ttgaggctat ggtcttttca taatcagagt caataataaa attggattga gtcaatccca
     1801 tgcctttcag catcatggtt atcaaaacca gtcctaaccc ggcaccttct ttatagtcat
     1861 cttcttgagc ctgaatgaaa taatcggtga tgtcctcnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnaac cagcttgata taatgttcaa tagattcgct gaccgaatat
     2041 gcttttataa taataggttt gttcaggttt attgcttcat cgatagtcat ggttaatccc
     2101 ttttaatccg ctatcgataa aataacactg taatggcagt atgtcaagta tttttattat
     2161 ttagttgtgt tacaacaggg acagcatgaa atgtcaaaag aaccatcggg atttctttgc
     2221 tgaatcccat attcaccctg catgaatttc tgattcatta taacgtacca gttttcaacg
     2281 ccaatttctt taattttcaa aagattatct atcactatat ggcggtaatg tgcaaagttt
     2341 tcaccgggat gatagttact gaatgtttta aggcgctggc aggggaaaag gtcgcattca
     2401 aaacaaaatt ccatcccacc aatgcgaaca cagcaatcgt aaatttcaca ggcatcatgg
     2461 aatgtatcag cccggcatcc tttgcaaata ccatatttgt aaaaccgggg gcatgcagca
     2521 caaaaaattc cacacggaga tgcaagatct tttttattca taccattgat agttaatttt
     2581 tattaataaa agagtatcat tttttttagt ctttgcaaaa aaattgctac actaatttaa
     2641 gaagaatctg taatacttat ataattatgt gcggcgatag ttactggtaa accatggtgc
     2701 agtgtaagcc aatttcagca atgttagcac agtcatatgt atttcacaca tatcaaaact
     2761 tcgtcatccc acgttgtgat ttctccagat gacggtgtgt attgaataca atgtaaaagg
     2821 aggcattgat ataatgaaaa aaagaatccc gtatgtatta gcaattatta cagtgctgtt
     2881 aggcacgtat accatctata gccagataaa gtggtatgat ttcaactctg gctttgcagc
     2941 ggtaaaaaat tcaaaaaagc cagcagtcat tgatttttat gcagactggt gtatctggtg
     3001 caaaactatg gataaggaaa cgttttccaa tcctgcagtt gaaaaaaaat taagcagaga
     3061 ttatatcgca atacgcataa atgttgaaaa gaatgatagc attacatttg aaaataaaac
     3121 ctatagtgca caggaatttg ccgcatattg cggagttgaa ggccttccaa cactggtatt
     3181 catggataag gaggggaagc tggtaacccg aatacctggt tatataaagg ctgaggtatt
     3241 cttatcattg cttgattata tgaaagatga atgttacaaa aagaaagtat cgtttcatga
     3301 atatatgcag caaaaagact gtgggaaata gtgaggtata gatggaattt atagaatggg
     3361 gtgaacatct aagtgtaggc gttacagttt ttgacaatga acacaaacag ctggtttcat
     3421 tggttaataa attaaatcat gccttgcttg caggcagcgc aaagaaaaca atggaagaaa
     3481 tattgcaaaa tctggtaaat tatacaaaaa ttcattttaa gcatgaagaa gattatatgg
     3541 tactctatga ttatccagaa tatgaaaaac ataaaaaaga acatgatgca ctgacagatc
     3601 aggtaatgga tttttatatg cggtaccagg cagggaaaac cgttttttca cttgaattaa
     3661 tgaatttttt aaaagattgg ctgacaaatc atattttaat cagcgacaaa aaatacaaaa
     3721 acttttttca gtcaaaaaat gttgaataat caagcttttg tcatagcctg aaccaatgta
     3781 taaaggttct gagcaatgtc tttgatagct gatgctacat tggatacagt attgacaata
     3841 tccgttagat tttgtgcagc atctgacaca taaacaatgg tcttggatgc ctcttcgttg
     3901 gcaattcgct gctcactggt tgatgtttca attgtttcga ttgcattgta tatctgagta
     3961 tttagattgg cgatagctct aatagataca ttaacatcct gaataagcgt attagtatgc
     4021 gatatttcct tcattattgt tcgtatttcc tcattaagtt tagataaaaa ttgtgatgta
     4081 tcagttatac tggtattact ggaacgcatc aggttttgtg tttgttcaat aatttttcct
     4141 atctgcgatg cattctgtgc ggtggcatca gccaattttg atatttcatc ggctactacg
     4201 gcaaagcccc tacctgcatc accagcacgt gctgcttcaa tggaagcatt aagtgataat
     4261 aaattgacct gatcggctat ctgattaata acttcaacaa acgaagacat attattgcta
     4321 aaagttataa gatcctgaaa ttgcttaaga gtatcatcca ttttctgtat agagtcattg
     4381 gataaagatg ttatggtttc aatggcatgc gctatggtac cggcgctttt tgtaatttct
     4441 tcaaatacct ttttcaggtc ggttactgat tcgtttgcaa tctgcatttc ctggtatagt
     4501 gagcgtgcag tgttgctaat attttcagca ttgctggtaa gttcttctac tgatgctgaa
     4561 atttcttcaa gcgaggcagc ctgtttattt gcaagatcat ttatggtatc taccacactg
     4621 gcctgttctt gtgatttttc gttcagaata ccaactgaat gttcaacgtt ctgggcaatg
     4681 acagcaatac gttcatgttt attatttgct tcatcagctt tgtcaagcgc caggtctaaa
     4741 agtgcacggg ttgcttttgt cccaaaatat gctgaaatac caacccatat atagattaaa
     4801 aacctgacgc caataatgct tcccactggg ccttcaggta ttaactgggg acgtaatatg
     4861 aaaagacata tctgcgagat aattaccgta ataaaagaaa aaatagctac tctggtgtca
     4921 aaataaaaaa cagaaagtcc cagaatgatg tagtgagcag caaaaagttc tcgtgagcca
     4981 tatataatat actgaaaaat gaaaagagaa agcataacag aaaaaattgc taaatatggt
     5041 gaaaaccatt tttgacggta tctggtaatg ataaagaaac caatagataa aataaatgat
     5101 accgataaaa atgcaacgat gatatcaatg agagtaagat attgagaacc tgtccctgtt
     5161 aaaaagataa tatatgttgc taaagttgct aaaattacaa tgctggtaaa tgctttgaat
     5221 aggcgcttat ggttattata tataatatcg ttaaataact ctttacgggt attattattt
     5281 gtcataataa tcaccacctt aataatgaat agttataaat actcatgaca gtattgtatg
     5341 aatagataaa actgtcaata aataattga
//
LOCUS       DAORYL010000127         5314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_10565_length_5314_cov_12.736832, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000127 DAORYL010000000
VERSION     DAORYL010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10565_length_5314_cov_12.736832"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="PLV81_14475"
     CDS             complement(<1..129)
                     /locus_tag="PLV81_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOM11343.1"
                     /translation="MGQKIKILFLCTGNSCRSQMAEGFANALRGDIIEAYSAGIEPK"
     gene            complement(211..1299)
                     /locus_tag="PLV81_14480"
     CDS             complement(211..1299)
                     /locus_tag="PLV81_14480"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOM11344.1"
                     /translation="MRFEWMGRQESQYVADTIGQVYIELSTACNLSCATCVRHSIKDF
                     KNTHFTKKLLNRLVSQLQELSNLNRIVLLGFGEALCNPDIQWHLRQLSTLNVPIVLVT
                     NGLLITQHVAELFVSLPLEAVYISIDDPAGVKPTIRKGSDVHIPLQAITLLKETKHNI
                     KAQKPIVGVETVVTQHNINSVVDIITMAKQAGADHCIVSHVFPYSSDMSNAIAYSIDG
                     KYDGLKQARKLFLHDSTVTIAGSNASTLRSCPFIEKGTVFINVEGYISPCPELAYTHL
                     AYYFGNPRTHIQHRFGNILKNNIAAIWESAEFVSFRDTFRYFEFPDCTLCVEPDMCYH
                     RTVEHKDCYWNTSPCGECLWGKNIVICP"
     gene            complement(1323..2366)
                     /gene="arsB"
                     /locus_tag="PLV81_14485"
     CDS             complement(1323..2366)
                     /gene="arsB"
                     /locus_tag="PLV81_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013876915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOM11345.1"
                     /translation="MADSVAKKLSFVDRYLTLWIFLAIALGVAMGYYLPGVKGFINTL
                     QVGTTNIPIAIGLILMMYPPLAKVKYEEMGKIFQNKKLLVVSLLMNWVIGPIVMFVLA
                     VLLLRSYPEYMIGVILVGLARCIAMVIVWNELAGGDRELAVGMVAFNAVFQVLFYAVY
                     IYLFITLALNWFGLVKGLNISVSMSESAKTVFIYLGIPFLGGVITRFGLIALKGKQWY
                     ETQFIPKISPITLIALLFTIIVMFSMQGNKIIERPQDVVIAAFPMLLYFVLMWFATFY
                     IVRRITGDYAKTTAVAFTAGSNDFELAIAVAIAIFGINSGQAFATVIGPLVEVPVLIG
                     LVNVALAFKKRYF"
     gene            complement(2438..3307)
                     /locus_tag="PLV81_14490"
     CDS             complement(2438..3307)
                     /locus_tag="PLV81_14490"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOM11346.1"
                     /translation="MFSKVLFASDLSEASTHVIECIKDLKVYGTKEIILFHALGIKYL
                     EDLRYSLVKEAEPVLQKQKAMLERYGYSVTVHIANDGVSYELWKFAKQENVSMVVIGT
                     HGRSLAFDVSLGGTAHKIIHDISKPLLVVRLRSDDKAMPVCQAACIDIDKPLLFTTDF
                     SDTAELAFTYVEKMAESGIKHVVLMHVQDKTRIEKYLKDKLNEFNTIDTERLEMLKDR
                     LVKKGVKNVDCILKYGIPVEEIINESRNRDYSLIIMGALGRSLVKDVFIGSVSWNVVR
                     NASQPVLLIPPLK"
     gene            complement(3403..3612)
                     /locus_tag="PLV81_14495"
     CDS             complement(3403..3612)
                     /locus_tag="PLV81_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOM11347.1"
                     /translation="MPAYEYRCDDCNETFTVIASMSEKEKGLQVVCKICGSTNVRQVY
                     SSISVGAKNKDLPIIGGGCSCGGAC"
     gene            complement(3657..4004)
                     /locus_tag="PLV81_14500"
     CDS             complement(3657..4004)
                     /locus_tag="PLV81_14500"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOM11348.1"
                     /translation="MITQVKVSTIFKMLSDTNRLRIINLLMQKELCVCHMAELLGITQ
                     TNVSHHLAKLHAASVVHYRKDGQWIYYSLSEDFVKDNHQLFEYLTQVFSLESIYKDDI
                     KTLHAMGDSHCRA"
     gene            complement(4104..4805)
                     /locus_tag="PLV81_14505"
     CDS             complement(4104..4805)
                     /locus_tag="PLV81_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOM11349.1"
                     /translation="MMELFTWLTNALYDTVWIAITASFVWGVLSILLSPCHLASIPLI
                     IGFIDDQGIRTTARAFVVSLMFSMGILITIALIGVGTAFAGRMMGDVGVWGNVFVACI
                     FFIIGLHLLEFINIPFLQKSANPAISKKGLWAAFLLGLLFGIALGPCTFAYMAPMLGV
                     AFQVASTKLLFAIALVIMYAIGHCSVIVVAGTSYEVVQRYLNWNERSKGALIVKKACG
                     VLVIAGGVYLIISVL"
     gene            complement(4802..5221)
                     /locus_tag="PLV81_14510"
     CDS             complement(4802..5221)
                     /locus_tag="PLV81_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOM11350.1"
                     /translation="MKKLLICILMIIVSVTIACKQNNNDVQTQQNTAVETNIMAETQD
                     KAIVTFVEIGSVNCIPCRMMQPVMKKVEEKYGSKVKIVFYDVWTQEGAPYAQKFGIRV
                     IPTQVFLDKNGKEFFRHEGYFPFEAIDELLQKQGLKP"
BASE COUNT         1717 a         1159 c          809 g         1629 t
ORIGIN      
        1 ttttggttca atgcctgctg aatatgcttc aataatatcg ccacgtaatg catttgcaaa
       61 accttcagcc atctggctac ggcatgagtt tcctgtacat aaaaataaaa ttttaatttt
      121 ttgacccatt gtatttccct ttccagtttt gatttaattt taattattat ctcacaataa
      181 aaaatagtaa ataaatattt tgtaaatttt ttatggacat ataacaatat tcttacccca
      241 caggcattcc ccacacggcg aagtattcca gtagcaatcc ttgtgttcca ctgttcgatg
      301 ataacacata tcaggttcca cacacaatgt gcaatcagga aattcaaaat acctgaatgt
      361 atccctgaaa ctgacaaact ctgcactttc ccatatagct gcaatattat ttttaagaat
      421 attcccaaat ctatgctgta tgtgtgtgcg cggattacca aaatagtaag ccaggtgtgt
      481 ataggcaagc tcaggacagg gagagatgta tccttcaaca ttaataaaca ctgtcccttt
      541 ttcaatgaaa gggcaactgc gcaatgttga tgcattgctt cccgcaatgg tgacagtgga
      601 atcatgtaaa aataatttcc gtgcctgttt tagtccatca tactttccat caattgaata
      661 agctattgca ttactcatat cactgctata tggaaatacg tgacttacaa tacaatgatc
      721 tgcccctgcc tgttttgcca tcgtaataat atcaacaaca ctgttaatgt tgtgctgtgt
      781 aacaacagtt tccacaccta ctatcggctt ttgtgctttt atgttgtgtt ttgtttcttt
      841 aagcaatgtt attgcctgca atggtatatg cacatcacta cctttgcgga tagtgggttt
      901 aactccagca ggatcatcaa tggatatata gactgcttca agaggcaaag aaacaaataa
      961 ttcagctaca tgctgtgtaa taagcaaacc attggtaaca agtactatcg gcacattcag
     1021 tgtactcaat tgtcgcagat gccattgtat atcgggatta caaagagcct caccaaatcc
     1081 caataaaaca atacggttaa ggttcgatag ttcctgtaat tgactcacca gcctgttaag
     1141 tagcttttta gtaaaatggg tatttttaaa atctttaatg gaatgacgta cacaggtggc
     1201 acacgataaa ttacaggcag tggaaagctc aatatatacc tgaccaatag tatcagctac
     1261 atattgactt tcctgcctgc ccatccattc aaaacgcata tgtgtatcta ctgaaacaag
     1321 ctctaaaaat atcgtttttt aaatgccaga gccacgttga caaggccaat caacacgggt
     1381 acttcaacaa gcggaccaat aaccgttgca aatgcctggc cggaattaat accaaatata
     1441 gcaatggcaa ctgctattgc cagttcaaaa tcattggaac cggctgtgaa tgccacagca
     1501 gttgtttttg cataatcgcc ggtaatacgc cgcacaatat agaatgtggc aaaccacatt
     1561 aacacaaaat atagaagcat tggaaacgct gctataacaa catcctgtgg gcgctcaata
     1621 atcttattcc cttgcattga aaacatgaca ataattgtaa agagcagtgc aattaatgta
     1681 atggggctta ttttaggaat aaattgagtt tcataccact gcttcccttt gagtgcaatc
     1741 aaaccaaagc gtgtgattac accaccaaga aatggtatgc ctaaatatat gaataccgtt
     1801 ttagctgact cagacataga aactgagata ttcagaccct taaccagacc aaaccagtta
     1861 agcgccagag ttataaacaa atagatatac actgcataaa ataaaacctg aaataccgca
     1921 ttaaacgcaa ccataccaac ggcaagttca cgatcaccgc cagcaagctc attccacaca
     1981 attaccattg caatacaccg tgcaagccca accagtataa caccaatcat atattcagga
     2041 tatgagcgca aaagcagaac tgccagtaca aacatgacaa ttgggccaat cacccagttc
     2101 atcagcaatg aaactaccag caatttttta ttctgaaaga tttttcccat ttcctcatat
     2161 ttaaccttag caagaggcgg atacatcatg aggataagcc ctatggcaat gggaatattg
     2221 gtagtcccaa cctgcaacgt attaatgaag ccttttacgc caggcaaata ataccccata
     2281 gcaacaccca gtgctattgc taaaaatatc cataatgtta aataccggtc aacaaatgaa
     2341 agcttttttg ctacactatc agccatagtt atacctcttt tacgtaataa ttatttcact
     2401 cagtgataat tcacattact tagtattact gattgtttta ttttaaaggt ggtattaaca
     2461 ataccggttg agaagcatta cgtactacat tccagcttac actaccaata aacacatcct
     2521 tcacaaggct tctacctaac gctcccataa ttatcaatga ataatccctg tttctggact
     2581 cattgataat ctcttctacc ggtattccat attttaaaat acaatcaacg ttttttacac
     2641 cttttttcac cagcctgtct ttcaacattt caagcctttc agtatcaatt gtattaaatt
     2701 cattaagctt atcttttaaa tatttttcaa tccttgtttt atcctgtaca tgcatgagca
     2761 ctacatgttt aataccactt tctgccatct tttcaacata agtaaatgca agctcagcag
     2821 tatccgaaaa atctgttgta aaaagcaagg gcttatcaat atcaatacat gcggcctgac
     2881 ataccggcat tgctttatca tcagaacgca aacgtacaac aagaagtggt tttgatatat
     2941 catgtattat tttatgagca gtgccaccta acgatacatc aaacgctaaa ctgcgcccat
     3001 gcgttccgat gacaaccatt gaaacatttt cttgcttagc aaatttccac aattcatatg
     3061 atacaccatc atttgcaata tgcactgtta ccgaataccc atagcgttca agcattgctt
     3121 tttgtttttg tagtactggt tcagcttctt ttaccagaga ataccgtaaa tcttctagat
     3181 atttaattcc taatgcgtga aataaaatta tttctttagt tccatacact tttaaatctt
     3241 taatgcattc aatgacatga gtggatgctt ctgacagatc agaagcaaat agtactttgc
     3301 taaacattgt tgcctccaga taaattttaa attttaacta cagtgtaatt caaaacacat
     3361 gttgtggtta tcaattcttt tcacagtaat ttatttgcat gatcaacatg ctcctccaca
     3421 ggaacaacca ccgccaataa tggggagatc cttattcttt gcaccaacag aaatacttga
     3481 atatacctgc cgtacattgg tgcttccaca tattttgcat accacctgca accctttttc
     3541 cttttcactc atagaggcaa tgacagtaaa tgtttcatta cagtcatcac aacgatattc
     3601 atacgctggc attgaaataa ttcctcctta tttaaaaaaa taataccttt taagctttat
     3661 gccctacaat gggaatcacc cattgcatgc agtgttttta tatcatcctt atatattgat
     3721 tccaatgaaa acacctgtgt aagatactca aataattgat gattatcctt aacaaaatct
     3781 tcactaaggc tataatatat ccactgccca tctttccgat agtgtaccac gctggctgca
     3841 tggagctttg ctaagtgatg agatacgttg gtttgcgtta ttccaagcaa ctcagccata
     3901 tggcacacac ataattcttt ctgcataagt agatttatta ttcttagcct gttagtatca
     3961 gaaagcattt taaatatggt tgatacttta acctgtgtta tcataccgat agtccatgtt
     4021 gcctgtatat atcaatatat gaacaaatat tcatatacta aacataccgc atattacttt
     4081 taaatcaatt ttttttcctt acattataaa acactaatga ttaaatatac tccaccagca
     4141 ataaccagca ctccacatgc ttttttaaca atcaaggcac cttttgaacg ttcattccag
     4201 ttaaggtaac gctgcaccac ttcatacgat gttcctgcaa caactattac actgcaatga
     4261 ccaattgcat acatgatcac cagagctatc gcaaataaaa gctttgttga tgctacctga
     4321 aacgcaacgc ccagcattgg tgccatatag gcaaaagtgc agggccccag cgcaatgcca
     4381 aacaaaagtc ctaacaaaaa ggcagcccac aagccttttt tactaattgc tggatttgcg
     4441 cttttttgta aaaatggtat attaataaac tccagtaaat gcaacccaat gataaaaaaa
     4501 atacatgcca caaatacatt gccccataca ccaacgtcgc ccatcatcct gccagcaaaa
     4561 gcagtaccca caccaataag tgcaatggtg ataagtattc ccattgagaa catgagtgaa
     4621 accacaaatg cccgtgctgt tgttctgatt ccctgatcat caataaatcc aataataagc
     4681 gggatacttg ccaggtgaca tggcgatagt agtattgata atactcccca tacaaatgaa
     4741 gcggtaatgg caatccatac agtgtcatac agtgcattgg taagccatgt aaaaagttcc
     4801 atcatggctt taacccctgc ttttgcaata actcatctat agcctcaaaa gggaaatagc
     4861 cttcatggcg aaaaaattct ttaccatttt tgtccagaaa cacctgtgtt ggaatcactc
     4921 gtatgccaaa tttttgagca tacggagcac cttcctgtgt ccatacatca taaaaaacaa
     4981 tctttacttt gctgccgtac ttttcctcaa cttttttcat aactggttgc atcatacggc
     5041 atggaataca attaaccgat cctatttcaa caaatgttac tatagcttta tcctgtgttt
     5101 cggccattat attagtttct actgctgtgt tttgctgtgt ttgtacatca ttattatttt
     5161 gtttacatgc gatagttact gatacaataa tcatcagaat acaaatgagt aattttttca
     5221 tgattaactc cctatgcaat agaaataaat tatttcatta tacatcaaac acgtctgcta
     5281 acagagattc ttccccttcg acaggctcag ggca
//
LOCUS       DAORYL010000128         5313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_10568_length_5313_cov_12.935717, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000128 DAORYL010000000
VERSION     DAORYL010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10568_length_5313_cov_12.935717"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PLV81_14515"
     CDS             <1..246
                     /locus_tag="PLV81_14515"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOM11351.1"
                     /translation="PEQLAKEITEALDIPTIGIGAGRYCNGQVLVINDMLGLNKGFNP
                     KFLKRYADLYTTSQQAIQTYCKEVKEHAFPAESNVFK"
     gene            complement(247..1149)
                     /locus_tag="PLV81_14520"
     CDS             complement(247..1149)
                     /locus_tag="PLV81_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11352.1"
                     /translation="MKKLYICVALLLVVLLYPVFPQTKQEIVFLYTNSLNGILDACQC
                     AADPKGGLVKRGYAITQLKKKYPNALLLESGDFCSYYPDTLLTKYIIKGYKHIGYTAV
                     GIGDQEFAAGVETFYQYRNELPFVCANLQYFAGGRWQATKKHITVTIGNKTIGITALI
                     DTDAFKYYPDEIVSKIKISEPKEALQKEIKNLAGNFIVVISHLGYEKDKELAVQFPQI
                     GLIVGGHSQTLVKKPVKVGSTLIVQAGPNGSRIGVLRAIVTDTKITGYEHSFVLPKWE
                     DPDDPIIEKMIAEYNEENAKRFPR"
     gene            1463..2629
                     /locus_tag="PLV81_14525"
     CDS             1463..2629
                     /locus_tag="PLV81_14525"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOM11353.1"
                     /translation="MPTNSLRNTIFRSIFIISGILLVVFTLFISALIYKSGKDNAYAI
                     IRQRNLAMKNYILGYFMPLRNTVEALSENPTIKKGSKVIAKDKQEILELYKIVKNMIP
                     NINYIYSGYEDKNLFINDYTPPAGFNPTTRPWYIKALESHPYVSDGIPYQEIKDKSWL
                     VSISKTITDVNGKIVGVLAIDTSLQHVMEQISASDFSLKTSYSYVINRNHVLIMHHDK
                     QYLNHSLSTLITPQPDFKEQEGSFSYSTIDGISKIAYYHRIDELGWILVTIVNKNEIL
                     LPLIFKITLVLGVVLIAAVVLGIITSNMLITNLVRPLVKLENYLTNPTKNEKQIQYPD
                     NEIGRLARAIQNITDEELYKKNQQANGYECSIRTSFYYRSPYRVIQQKEDITGN"
     gene            2547..3071
                     /locus_tag="PLV81_14530"
     CDS             2547..3071
                     /locus_tag="PLV81_14530"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOM11354.1"
                     /translation="MNAQLELLSITDPLTGLYNRRKILQEIERESLRVNRYNCNASIL
                     IFDIDHFKTINDNYGHIEGDLILKELGELIKSLIRTTDIFGRLGGEEFIILFPETNLA
                     SAAIIAEKLRKAVEEHIFTNGKNITISIGVGTFIQGTSIDDVIKEADNMLYKAKKGGR
                     NRVEYINKENEQTP"
     gene            complement(3082..4440)
                     /gene="csx2"
                     /locus_tag="PLV81_14535"
     CDS             complement(3082..4440)
                     /gene="csx2"
                     /locus_tag="PLV81_14535"
                     /inference="COORDINATES: protein motif:HMM:NF021006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02221 family CRISPR-associated protein"
                     /protein_id="HOM11355.1"
                     /translation="MAKALLSFLGTNKYIKCNYRYHDTVIYGVHYVQEAIAKIFCNEW
                     SQGDSIIIFVTKEAEEENWEIGKDPENGEIEGLKSVLKKFADNKKIEVRHVPIPGGQN
                     DSEIWGIFDAVINSVEKGSEIIVDVTHAFRAIPMLAVVMINYLHMVKNVTFNGLYYGA
                     VESLGKIEDIKKMPVSERTAPIIDLSSFIYLLQFSNAVNNFMQYGDAEEIHTFTLNAV
                     TPVLRETSGQHDISRRIRRVADDLRKMTQNFAYCRGKDIIKFNYARLYSEIKELKESF
                     GQDQQLLPLKPLQPLLDNILKKIEIFKDYGKEENAVKGILAAQWCLEHNLIQQSITIL
                     QESIITLICMRHNLKYSKKDDRELIASAFYKVIDQKNPKTKKDGRKEKSEEGKIQALL
                     VDQMIHSLGKTYHKLSQLRNDINHANFREDEKEPEDIYNEIIEIHKKVMDEVNRTLRL
                     RS"
     gene            complement(4465..4860)
                     /gene="csx20"
                     /locus_tag="PLV81_14540"
     CDS             complement(4465..4860)
                     /gene="csx20"
                     /locus_tag="PLV81_14540"
                     /inference="COORDINATES: protein motif:HMM:NF040559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Csx20"
                     /protein_id="HOM11356.1"
                     /translation="MISMCVLMNHRMTESQIEDAVNALKVEKFIFPPENLQKIWEQIP
                     ADNEDILDIVEPVLQWLLDNSNEGDYIFVQGDFGATLLVVSWAFKHDRIPVYSTTQRK
                     YESTINEKGEVVNVHTFKHVRFRRYRMIQ"
BASE COUNT         1728 a          987 c          814 g         1784 t
ORIGIN      
        1 cccgagcagc ttgcaaaaga aataactgaa gcgcttgata tacccaccat tggcattggt
       61 gcaggacgtt actgcaatgg gcaggtcctt gtaattaacg atatgttagg cttaaacaaa
      121 ggatttaatc caaaattctt aaagcgctat gctgaccttt ataccacatc acagcaggca
      181 atacaaacgt actgcaaaga agtcaaagag cacgcattcc ctgcggaaag caatgtgttt
      241 aaataatcag cgcgggaagc gtttggcatt ttcttcatta tattcggcaa tcattttttc
      301 aattatgggg tcgtcaggat cttcccactt tggcaataca aacgaatgtt cataacctgt
      361 gatttttgta tcggtaacta tcgcccgtaa cacaccaata cgagaaccgt ttggtcctgc
      421 ctgtacaatt aatgtgctgc caactttcac gggcttttta acaagcgttt gcgaatgtcc
      481 tccaacaata agcccaatct gcggaaactg aacagccagc tctttatctt tttcataacc
      541 cagatgggaa ataacaacaa taaaattgcc cgctaaattt tttatctctt tttgcaaagc
      601 ctctttgggc tctgaaatct ttatttttga cacaatctcg tctggataat acttgaatgc
      661 gtcagtatca ataagtgcgg taatgccaat tgttttgtta ccgatagtta ctgttatatg
      721 tttctttgtt gcctgccacc tgccacctgc aaaatattgc agattagcgc acacaaatgg
      781 caattcattg cgatactggt aaaacgtttc aacgcctgca gcaaattcct gatcgccaat
      841 gccaacggca gtatagccaa tgtgtttata gcctttgatg atatatttgg taagcaatgt
      901 atcaggatag taactgcaga agtcaccgct ttcaagaagc agtgcattgg gatatttttt
      961 ctttagctgg gtgatagcat atccgcgctt aacaagtcca ccctttggat cggcagcgca
     1021 ctgacaggca tccagtatgc cattgaggga attggtgtac aaaaaaacaa tttcctgctt
     1081 agtttgagga aacacagggt ataataatac taccagtagt agtgcaacac aaatatatag
     1141 ctttttcata aaactgctcc catcatgtaa ctatgatata ttatgaaatt cattacgctc
     1201 ttaattattt catttgaata acatttgatg tgcgagttac ttgcttgagt tcaattttca
     1261 attatttttt tgttttttga taaaaggggt tctcaatatt tttggaatat gcttgcaaaa
     1321 gcgatggaat ctagtacaat gtttattgaa agcgtctatt tcacaatatt cgataattaa
     1381 catttaaaaa tatgaaatgt ttttttatta caatactgta attaattaat cacaattatc
     1441 ttaacgtaaa aaaaatttaa ttatgcccac taatagttta agaaacacca tttttagaag
     1501 tatttttatc atatcaggaa tattgcttgt tgtctttaca ctatttattt ctgctttgat
     1561 ttacaaatca ggtaaagata atgcatatgc cataatcagg caaagaaatc ttgcaatgaa
     1621 aaattacatc cttgggtatt ttatgcctct tagaaataca gttgaagcat tatcagaaaa
     1681 tccaacaata aagaaaggat caaaagttat agccaaagat aaacaagaga tacttgaatt
     1741 gtacaaaatt gtcaagaata tgatccccaa tattaattat atttattcag gctatgaaga
     1801 taaaaatctt tttataaatg attacacacc acctgccgga tttaatccca caacacgccc
     1861 atggtacatt aaagcacttg aatctcatcc ctatgtttca gatggcatac catatcagga
     1921 aataaaagat aaatcatggc tggtatctat tagcaaaaca ataaccgatg tcaatggcaa
     1981 gattgttgga gtgcttgcta ttgatacatc attacagcat gtaatggagc aaattagcgc
     2041 aagcgatttc agtctaaaaa cttcatatag ttatgttata aatagaaatc acgtactcat
     2101 aatgcaccat gataaacaat atctgaatca ttcgttaagc acattaataa ctccccaacc
     2161 tgattttaaa gaacaggaag gaagttttag ttatagcact attgatggaa taagcaaaat
     2221 agcatactat cataggattg atgaacttgg atggattttg gtaacaatag taaataagaa
     2281 tgaaattctt ttacccctca tttttaaaat aaccctcgtt cttggagttg ttcttattgc
     2341 agctgttgta ttgggaatca taacaagtaa tatgttaata acaaatttag tacggcctct
     2401 tgtaaagcta gagaattatt taactaatcc cactaaaaac gaaaaacaaa tccaatatcc
     2461 agataatgaa ataggaagac ttgctcgtgc aattcaaaat atcactgatg aagagcttta
     2521 taagaaaaac cagcaagcta atggatatga atgctcaatt agaacttctt tctattacag
     2581 atccccttac agggttatac aacagaagga agatattaca ggaaattgaa agagaaagct
     2641 taagagtgaa cagatataat tgcaatgctt ccatactcat atttgatata gatcatttta
     2701 aaactataaa tgataattat ggacatattg aaggagatct tatcctcaaa gaactgggag
     2761 aacttatcaa atctttaatc aggacaacag atatttttgg cagattgggt ggagaggaat
     2821 ttataattct ttttccagaa acaaatttgg cttctgctgc tattattgca gaaaaactaa
     2881 gaaaagctgt agaggagcat atatttacca atggtaaaaa tataacaatc agtattggag
     2941 ttggaacatt tatacaaggc accagtattg atgatgttat aaaagaagca gataatatgc
     3001 tctataaagc aaaaaaaggt ggtagaaata gagtggaata cataaacaaa gagaacgaac
     3061 aaacgccttg aaaatgcaaa ttcaggaacg gagcctaagt gttctattaa cttcatccat
     3121 aactttttta tgtatttcaa ttatttcatt ataaatgtct tcaggttctt tttcatcttc
     3181 tctaaaattt gcatgattaa tatcgttacg aagctgagat aacttatgat atgttttgcc
     3241 taacgaatgt atcatttggt caactaataa agcttgaatt tttccctctt ccgatttttc
     3301 tttcctacca tccttttttg tttttggatt tttttgatca atcactttat aaaaagcact
     3361 agcgatcaac tctctatcat ccttttttga atattttaag ttatgcctca tgcatatcaa
     3421 tgttattatt gattcctgta atatggtaat gctttgctgg attaaattgt gttccaaaca
     3481 ccattgcgct gccaggatac cttttacagc attttcttct ttgccatagt ctttaaatat
     3541 ttcaattttt ttcaaaatat tatcaagcaa tggttgtaat ggttttaatg gtaatagttg
     3601 ttgatcctgc ccaaaacttt ctttaagttc tttaatttca ctgtataacc ttgcataatt
     3661 aaacttaatt atatctttgc cacgacaata tgcaaaattc tgtgtcattt ttcttaaatc
     3721 atcagccact cttcttattc ttcttgaaat atcatgttgc ccactcgttt ctcttaaaac
     3781 tggcgttact gcatttaagg taaaagtgtg tatttcctca gcatcaccat attgcataaa
     3841 gttatttact gcatttgaaa actgtaataa ataaataaaa ctactcaaat caataattgg
     3901 tgccgtacgt tcactaacag gcattttttt aatatcctct atctttccaa gtgattcaac
     3961 tgcaccataa tataatccat tgaaggtaac gttttttacc atgtgaagat aatttatcat
     4021 cactactgca agcataggta ttgccctgaa tgcatgtgtt acatcaacaa taatttcact
     4081 gcctttttct acagaattaa ttaccgcatc aaatattccc catatttctg agtcattttg
     4141 cccgcctggt atgggcacat ggcgaacttc aattttttta ttatccgcaa atttttttaa
     4201 cacactttta agtccttcaa tttctccatt ttcaggatct ttgcctattt cccagttttc
     4261 ttcttccgct tctttagtta caaatatgat aattgaatct ccctggctcc attcattgca
     4321 aaatattttt gcaattgctt cctggacata atgaactcca taaataacag tgtcatggta
     4381 cctgtaatta catttgatat atttgtttgt gccaagaaaa ctcaacaagg cttttgccat
     4441 tcttgttctc ctaattttat aaatttattg tatcattcta taccttctaa acctgacatg
     4501 tttgaaggta tgaacattca caacttcacc tttttcattt attgtgcttt catactttct
     4561 ctgcgtggtt gaatatacag gaattcggtc atgtttaaaa gcccatgata caaccagcaa
     4621 tgttgctcca aaatctccct gaacaaaaat ataatcacct tcatttgaat tatccaaaag
     4681 ccattgtaaa acaggctcaa caatatccag aatatcttcg ttatccgctg gtatctgctc
     4741 ccatatcttt tgtaaattct cgggtggaaa aataaatttt tctactttta aagcatttac
     4801 cgcatcctct atttgacttt ctgtcattct atgattcatc aatacacaca ttgaaatcat
     4861 acatattccc ttgataaaaa tattatttaa aatgagtcgt tgcaactatt ttatttttag
     4921 ttaacattat aggaattgcc cttgctatca atgcaatcct ttgtccaatt tatcaagaat
     4981 gatgcaattt ccttcatagt gaaaataaat gcgtctggca tcagcatcgc ttcaggcatc
     5041 gcattcatat tgaaaaatgc aaagtaaatc cccacaacac tactctgaac atcattcatc
     5101 ctattatcta gtgtcatact atagtgaaca ctctgtcaac ttgaggagtc ccactctttt
     5161 tggacaacga aggatcttgc cgttcaactc atttaacaac gttttctatg gtcaatattt
     5221 cataacaaca ctgcaggcgc ttgagcgcct gcgattggta tatcattcca cattcagaca
     5281 aaaaaaatcc cctatcagcg gggtgcttcg aca
//
LOCUS       DAORYL010000129         5255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_10720_length_5255_cov_6.908269, whole genome shotgun sequence.
ACCESSION   DAORYL010000129 DAORYL010000000
VERSION     DAORYL010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10720_length_5255_cov_6.908269"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2083)
                     /gene="polA"
                     /locus_tag="PLV81_14545"
     CDS             complement(<1..2083)
                     /gene="polA"
                     /locus_tag="PLV81_14545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOM11357.1"
                     /translation="MPEKTIFVVDGHALCYRAYFALQRNPLINSKGQNVSAIYGFFRM
                     LLRLLQTQKPDYTIIAFDPPKKSFRFSMYPEYKAQRAKMPDDLRSQIEEIKAILQEMG
                     FTVVIMDDYEADDVLGSIARQYSDSYKVVLVTGDKDAYQLLSDKNVSIYAGKKGITEF
                     ESIDADAVVEKLGVRPEQIIDYMSLTGDAVDNIPGVKGIGEKTASKLLQQYGTLDNIY
                     KHLHELTGKIKEQIESQKDSAYLSRQLVTIKTDIPLSLSIEKALTPDLHNEKVRQIFK
                     NLEMDSIARELGDTEKSISIKEIAGYSTVTDIDTLNNVIAAIKNAGLVSFDTETTSSR
                     PMEAELVGVSFSIAPQSGWFIPVGDSSLWGYNAVPKDQLLQKIKTILEDDTIKKVGQN
                     IKYDMLIMLNCGITLTGIYFDTMVASYLLNPGLRRHNLDDLAAEYLNYTKITYDELVK
                     DGKKVQHITQVPLQKLSHYAIEDADIALRLYHILYKKLEQENLLELFFNVEMPLVTVL
                     AYMEYYGVKIDLEHFETLSKELSHKIKQTEEKIYSLAGIPFNINSTRELSHILFEKIG
                     LKPVKKTKTGFSTDIQVLEALRGQHEIIDYLMEYRTLSKLQTTYVDTLPALVNPKTGR
                     IHTSFNQTVTATGRLSSSDPNLQNIPVRDEFGKNIRAGFVVEKGCLLLSADYSQIELR
                     LAAHLSGDPTMI"
     gene            complement(2055..4499)
                     /locus_tag="PLV81_14550"
     CDS             complement(2055..4499)
                     /locus_tag="PLV81_14550"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOM11358.1"
                     /translation="MKFVFVNILLTVTIALKFFGSAFANQVHTAKPLHIRVVTAIYEP
                     FVMYKDGKLVGFDIDLLNEICKHNNISYTIHVTSFQDMLQQVKEGEADMAIGCIYVTE
                     ERERWFNFSESYLEGGLVLVLPADSSVRTIYDLAHMTVGVKKDASGDKYAMKLVAQGY
                     DISIVRFQDTVESFNALIAGRLDAVLNDYLNSVYLLNKYFSGSIKIAKGNWGDIVYDR
                     KKIAFPVSIHNKELLNIVSDSIHELQSKNFIDMLYWKWFAVMPPPDIKKRVMVYIGII
                     VITVLVSFLVFQLYERKKRIHKLQLLETHFRNLINDLNVGVYILQKNAIVLTNYEGAR
                     IAGTPLESIMGKTLDDYFDDYSVAEGIIAQYSSFNEMKNVLSSHHIHRAVWKRKDGVN
                     LTVVFRIRPIMWMGQGAVECVVNDTTELTHLLVKQEQLKGQLLQSQKLEIIGQIAGGI
                     AHDFNNILTVILGYANLVEKSIDDINKPAIEKIIQAGNHAKDIVAKLLTFSRRKAFHP
                     GEIHLHSIIHTAVSLLHTALSKNCEVVQLLHAFNDSISGDATEMQQVIMNLIINAAEA
                     MPHGGTITIQTENTTVANEKVGLVETIPAGDYILLSISDTGTGIAPEHLDHIFEPLYT
                     TKEKGSGLGLAIVYAIVKRHKGFIDVMSTPGSGTSFYIYLPVQPLHNVADETQNHALQ
                     AGTGTIIIIDDDDNICLLYQEALKLGGLQAITFTNPNEAFAYIDAHKDTIMLVVSDVR
                     MPTVSGEDVLDYIKNNYPDIPVVLMSGYYDDTMKEQLLRKGACQVWQKMYDIHEIVHK
                     VKGVLNNYARKNNIRS"
BASE COUNT         1525 a         1105 c          890 g         1735 t
ORIGIN      
        1 caatcatggt ggggtcacct gaaaggtggg cagctaaccg caactcaatc tgcgaataat
       61 cagccgataa cagaaggcat cctttttcca ccacaaatcc cgcccgtata tttttcccaa
      121 attcatcacg cacagggata ttttgcaaat ttggatcaga tgatgaaagc ctgccagttg
      181 cagtcactgt ctgattaaac gatgtgtgaa tcctaccggt ttttggatta accagtgcag
      241 gcagtgtatc aacgtatgtt gtttgcagtt tgctaagcgt acggtattcc attaagtaat
      301 caataatctc gtgctgtccc ctcaaagctt ccagcacctg aatatcggtg gaaaaaccag
      361 tttttgtttt ctttaccggt ttaagcccaa tcttttcaaa caatatgtgc gatagttccc
      421 gcgttgagtt gatgttaaat ggaattcctg ccagtgagta aattttttct tcggtttgtt
      481 ttattttgtg cgatagttcc ttagataatg tttcaaagtg ttcaaggtca atctttactc
      541 cgtaatattc catgtacgca agtacagtaa caagtggcat ttcaacgttg aaaaacaatt
      601 caagcaggtt ttcctgttct aactttttat agagtatatg gtataagcgc aaagcaatat
      661 cagcatcttc aatagcgtag tgggacagtt tctgcaatgg cacctgcgta atatgctgca
      721 cctttttgcc atctttcaca agttcatcat acgttatctt tgtataattg agatattctg
      781 ctgctaaatc atccagatta tgccgccgta atccgggatt aagtaaatat gatgcaacca
      841 tggtgtcaaa atagatgcct gtaagcgtaa taccacaatt aagcataatc agcatgtcat
      901 actttatatt ttgaccaact ttctttattg tatcatcttc aaggatagtt ttaatttttt
      961 gaagcagctg atcttttggt actgcattat aaccccacag ggaactatcg cccactggaa
     1021 taaaccaccc tgattgcggt gcaatggaaa acgatactcc cacaagctct gcttccattg
     1081 gtcgtgatga tgtagtttcg gtatcaaatg acacaagtcc tgcgtttttg attgctgcaa
     1141 tgacattgtt cagtgtatca atatctgtta ctgtggaata tcctgctatt tccttaattg
     1201 aaattgattt ttctgtatcg cccaattcac gggcaattga atccatctct aaatttttga
     1261 aaatctgccg aactttttca ttgtgtaaat caggggtcaa agccttttca atggaaagtg
     1321 ataacggtat atcagttttt atcgtcacaa gttgacgtga aagatacgct gagtctttct
     1381 gtgattcaat ctgttcctta atttttccgg taagttcatg caaatgctta taaatattat
     1441 ctaacgttcc atactgctgc aaaagttttg atgcagtttt ttcgccaatg cctttaacac
     1501 cagggatatt atctacagca tctccggtga gtgacatata gtcaatgatt tgttctggtc
     1561 taacacctaa tttttcaaca acagcatcag catctatgct ttcaaattca gtgatacctt
     1621 ttttgccggc atagattgag acgttcttat cgctcaaaag ctggtaagcg tccttatcac
     1681 cggtaacaag taccactttg tagctatccg aatactgacg ggcaatagaa ccaagcacat
     1741 catcagcttc ataatcatcc attatgacaa cggtaaatcc catctcctgc aatatagctt
     1801 tgatttcttc aatctgactg cgcaaatcat caggcatttt tgcacgctgt gctttatact
     1861 caggatacat agaaaatcgg aatgattttt ttggcgggtc aaacgcaatg attgtgtaat
     1921 caggtttctg cgtttgcaac aaacgaagca gcatcctgaa aaatccatag atggcagaaa
     1981 cattctgccc tttgctgtta attaaaggat tccgttgcaa tgcaaaatat gccctgtagc
     2041 ataatgcatg gccatcaact acgaatattg ttttttctgg cataattatt taacacacct
     2101 tttactttat gtactatctc atgaatatca tacatctttt gccatacctg gcaggcaccc
     2161 ttacgcaata attgctcttt cattgtatca tcataatagc cgctcatcag tacaacgggt
     2221 atatctggat aattattttt tatgtaatca aggacgtctt caccggaaac tgtcggcatt
     2281 cgtacatcgc ttacaacaag cattattgta tctttatggg catcaatata cgcaaatgct
     2341 tcgttgggat tagtaaatgt tattgcttgc aagccaccta atttcaatgc ttcttgatat
     2401 aaaagacaaa tattatcatc atcgtcaata ataattatag taccagtgcc tgcctgcaag
     2461 gcatggtttt gtgtttcgtc agccacattg tgtaatggtt gaacaggtaa atatatataa
     2521 aagcttgtgc ctgaacctgg tgttgacatt acatcaataa aacccttatg ccgctttaca
     2581 atagcatata ctattgccaa tcccaatcca cttccttttt cttttgtagt atacagtggt
     2641 tcaaaaatat ggtcaaggtg ctcaggagca attcctgtcc cagtatcgct aatactcagt
     2701 aatatataat caccagcagg tatagtttct actaagccaa ctttttcatt agcaacagtt
     2761 gtattttcag tctgaatggt aatggttcca ccatggggca tggcttcggc agcatttatt
     2821 ataagattca tgattacctg ctgcatctca gtagcatcac cagatatgct atcattaaac
     2881 gcatgtaaca gctggacaac ctcacaattt ttagacaaag cagtatggag caatgaaaca
     2941 gcagtgtgaa tgatactgtg caaatgtatt tcaccgggat ggaatgcctt acggcgtgaa
     3001 aacgttaaaa gttttgccac aatatctttg gcatgatttc cagcctgaat aattttttca
     3061 atggcaggct tatttatatc atcaattgat ttttcaacaa gattggcata ccccaatatc
     3121 acggtaagaa tattattaaa atcatgtgct atcccaccag caatctgtcc aattatttct
     3181 aatttttgcg attgcaataa ctgtcctttt aattgttcct gtttaaccag caaatgtgta
     3241 agctcggttg tatcatttac cacacactca actgccccct gtcccatcca cataattggc
     3301 cttatcctga aaactacagt aaggttaaca ccatcctttc ttttccacac tgctctgtga
     3361 atatgatgac tggataacac attcttcatt tcattaaacg atgaatactg tgctatgatt
     3421 ccctctgcaa cagaataatc atcaaaataa tcatccaatg ttttacccat aatagattct
     3481 aacggggtac cagcaattcg cgctccttca taatttgtaa ggactattgc atttttttgc
     3541 agtatataca ccccaacatt taaatcatta atcaaatttc tgaaatgtgt ttcaagtaat
     3601 tgaagtttgt gtattctttt ttttcgctca tacagttgaa atacaagaaa tgaaacaagt
     3661 actgtaataa caattattcc aatatatacc attacgcgct tttttatatc aggcgggggc
     3721 ataacggcaa accatttcca gtacagcata tcaataaaat tcttagactg taattcatgg
     3781 atactatcgc ttacaatgtt taataattct ttattatgta ttgacacagg aaatgcaatt
     3841 tttttacggt catatactat atcgccccag ttgccttttg caattttaat actgcccgaa
     3901 aaatatttat taagtaaata cactgaatta agataatcat ttaaaacagc atccagccta
     3961 ccagcaatta gcgcattaaa tgattcaaca gtatcctgaa agcggactat gctaatatca
     4021 taaccttgcg ctacaagctt catggcatat ttatcacccg aagcatcctt tttaacaccc
     4081 actgtcatat gtgcaaggtc atatattgtg cggacactgc tgtcagcagg aagcaccagc
     4141 acaagtccac cttctaagta actttctgaa aaattaaacc agcgttccct ttcctcagta
     4201 acatagatac agccaatggc catatcagct tccccttcct taacctgttg aagcatatcc
     4261 tgaaatgaag ttacgtgtat tgtgtagcta atgttattgt gcttgcatat ttcattcagc
     4321 aggtcaatgt caaatccaac aagttttccg tccttataca tgacaaatgg ctcataaatg
     4381 gcagtaacaa cacgaatgtg caaaggctta gcagtgtgca cctggttagc aaaagcacta
     4441 ccaaaaaatt tcagggcgat agttactgtt aatagaatgt ttacaaatac aaatttcatg
     4501 ggaaaatagt ataaaaaacc acggtatttg tcaaacgtta ttgctgcgca ttataatgat
     4561 tgctctgacc ccattatttt aattacacag gcgcttgtgc acctgcgaat gttgattgat
     4621 ggtttaccat atatgatact gttgccagcg tgctgcggat accctcttaa tgctttgacc
     4681 ccttgccttt aatgtattga aaagaagttc ttaagcatgt tgaagtgggt tcctgagtga
     4741 tttgacaagc tcactaacca ccgttgaagg attgcacgca atcttatgtg ctttgatata
     4801 ttgtctacgc tgtcttgcat aactttaggt atacaactta atgctctgac cccatccttc
     4861 ttactaaccc tgtgttttct gcgtgtttaa ttactttacc tcacatataa ccgctgagac
     4921 tacatgttat aatgctctga cccctattta acagcactat ctgtaacttg tgcgttctgg
     4981 gattgaatct tctgttggtt atcatgctct gacccttttg accccttcgt ttggatgcgc
     5041 catttgcctt attttaaggc tctgacccca ttttttttta ttttttttat aaatcttaca
     5101 aaatttttca ctttcaccgt tattttttcc ctcctcatgc gttgtatata gagaaaccac
     5161 tttacactat atatggagga ttactatggc tcggaaatta cgtgttcaac taccaggatt
     5221 aacctaccac atcacgtccc gctgcatcga atgga
//
LOCUS       DAORYL010000130         5220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_10811_length_5220_cov_12.966118, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000130 DAORYL010000000
VERSION     DAORYL010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10811_length_5220_cov_12.966118"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLV81_14555"
     CDS             <1..227
                     /locus_tag="PLV81_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11359.1"
                     /translation="TLLSAISEYKSKDYIERLQYRLRARLESFVGQYGRLTDDIELLL
                     KPLVKGNSIVIDNTEYVEEVITTVYNKLMP"
     gene            complement(312..968)
                     /locus_tag="PLV81_14560"
     CDS             complement(312..968)
                     /locus_tag="PLV81_14560"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOM11360.1"
                     /translation="MKTKIFYSVIAIMIFALAISCGNKYGGKWIAKIDSDKITDNELN
                     AYYYAQMKSIYNLPKEEIDKLAQDPAQLERNPLLNKSNFLEQMIQQRLVYKKAIDDGI
                     LKNEELNTLLDISKEGLVVQYYIREKFKNDITIAPQEVEQIYNQQRARFKGVPVDQAE
                     MYIKQQLFQQKLNMKIKELVDTLRDEKKIEKNMELLRKELNTQAQSQETQQAPQQPAK
                     "
     gene            complement(1073..>2203)
                     /locus_tag="PLV81_14565"
     CDS             complement(1073..>2203)
                     /locus_tag="PLV81_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOM11361.1"
                     /translation="HMALAGIRDLSIIATPPENRQSIETLVIEDNPDIVRMAILKEIE
                     RKGQVFYVHNRISTIYEQAQYLEKLVPEATFCVAHGRMHEHELEEVMIDFINGKYDVL
                     VSTSIIESGLDMPNVNTIIVNRADTFGLSQLYQLKGRVGRSDRQAYAYLFYPRDTHIT
                     EDAQKRLQVIAEYTEIGSGFKIAMKDMEIRGSGNILGKEQSGNIMDVGFDLYCQMLED
                     AVRKLKGEKPVHVFRTPVYVKTNIFIPDSYIADEKQKIEFYKRYEACESLEELTELTR
                     EMIDRFGPPPEEVKSLIAMEEVRTLASQLYIDEIIEDTKLVKMRITKESKLDTKKLVA
                     LIQKDKRLSIDRYDREIVNFVTGAIPPEKKLEEIKKLLQQLL"
     assembly_gap    2204..2303
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2304..4369)
                     /locus_tag="PLV81_14570"
     CDS             complement(<2304..4369)
                     /locus_tag="PLV81_14570"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOM11362.1"
                     /translation="MIHTIKLIYTILIVYTIYTLLLTIMVLKELTNIYSSTDEFKSLA
                     ASGVKAFSIEGIHPSAFPFIVATSFNTLQTQTIVVTTDYHTMNRFVADISAFVNEDTI
                     FPFPPLEVLPYEYVNPSSSIERDRITALYQLLSGKPVIVVATIESLLRAIPHKEFFLK
                     KGIHIERNQEYPFEVIIETLINYGYERETRVDRYGQFSVKGGIIDIYLPNAANPVRLD
                     FFGDTCESIRYFDPMTQVSLGEVETITIYPRREILLSHSEKEKIQKELLRGDSNRNQQ
                     AVESLQKANVPGIYDLFPLAIQTDTITSFAHNPHIFLCDPPQLKAKLSEIERTYHQLY
                     NHKKDSTFCIPASQLLLSDSYQHIESQAVKLHPFITDSKNVWHLKGLPSFQGRIQDVR
                     NECAKRIEEGWHIVVSTSFEGQARRLYDLFNEFKPQEHFDTYNPKKHFGIIISNLHEG
                     LEIGSIKTCIITDHEIFGKSYRKKKHFKQKYSKPIETFLELSPGDYVVHINHGIGIFK
                     AIERMSAGGVERDFLVIEYADGDKLYVSLDQINMVQRYVGLDGRQPRIDALGKKSAWN
                     RIKERVQQSVEEIAQDLLKIYSQRKALKGFQYPADTMWQEEFESKFEYEETPDQITAI
                     EDVKDDMEKSQPMDRLVCGDVGFGKTEVAIRAAFKAVMAGKQVAILVPTTVLAMQHYT
                     TFCKRFA"
     gene            complement(4459..5136)
                     /locus_tag="PLV81_14575"
     CDS             complement(4459..5136)
                     /locus_tag="PLV81_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11363.1"
                     /translation="MRLTISILMLLTCFTYSFAQINESAQPLQYPAGDISNTRPYIIW
                     CDIYNTSNRPFMVMVTITNSQGQSNEYTLYPEIIDGMYCVVQIPIALTPDTYTYTIKL
                     LHNNKPENKRYYHHKKYPVEGTFTVDTTKPDPVGSLDKTSLIYYLSQSRQNKLHYGYN
                     ALFFSSSGTISLGTGVAVYYLTNFGIVTTIISAVAITSGVIGITAGIYYGVKYYHNKN
                     VIENVIK"
BASE COUNT         1406 a          976 c         1020 g         1718 t
ORIGIN      
        1 gtacattgct ttcagcaatc agtgaatata agtctaagga ctacattgag cgcttgcagt
       61 atagattgcg tgcgcgattg gaatcgtttg ttgggcaata cggcaggttg actgatgaca
      121 ttgaattgct gcttaagcca ctggttaaag gcaatagcat tgtgattgat aatactgaat
      181 atgtggaaga agtaattacg actgtatata ataagcttat gccgtaattg tttgcatcca
      241 agccattggg caattgatat aaaaaagctg ctcacacagg agcagctttt taaacagtat
      301 attacattgt gttactttgc tggttgttgt ggagcctgct gagtttcctg actctgagcc
      361 tgcgtgttca attcttttcg cagtaattcc atgtttttct caatcttctt ttcgtcgcgt
      421 aatgtatcaa ctaactcttt tattttcatg ttgagttttt gctggaaaag ctgctgcttg
      481 atatacattt cagcctgatc aacagggaca cctttaaaac gagcccgctg ctggttgtat
      541 atttgctcaa cttcctgagg tgctatagta atatcatttt taaacttttc acggatatag
      601 tactgtacta ctaggccttc tttagaaata tctaacagag tatttaactc ctcatttttt
      661 aaaataccat cgtcaatagc ttttttgtat accaggcgct gctgaatcat ctgctcaaga
      721 aaattgcttt tgtttaacag agggtttcgc tcaagctgtg ccgggtcctg agctaattta
      781 tcgatttctt ctttagggag attataaata cttttcatct gtgcatagta gtatgcattg
      841 agttcattat cagtgatttt atcgctgtca atctttgcaa tccatttacc gccgtattta
      901 ttgccacagg agattgcaag tgcaaaaatc ataatggcaa ttactgaata aaaaatctta
      961 gttttcatag tatccatcct tttgatagta agaattaaaa aatatgcctc cagcggaggg
     1021 aattatcatt tactctttgt aaagatataa agtttttacc attggtacta gattatagta
     1081 attgttgcaa caattttttt atctcttcta attttttttc tggaggtatt gctcctgtaa
     1141 caaagtttac tatctccctg tcatacctgt caatggacag ccgtttatct ttttgaatga
     1201 gtgcaactaa ttttttagta tcaagctttg attctttagt aatgcgcatt tttactaatt
     1261 ttgtatcctc aataatttca tcaatgtaga gttgcgaagc caatgtccgt acttcttcca
     1321 tggcaatcag ggattttacc tcttcaggtg gcggcccaaa gcggtcaatc atctctctgg
     1381 taagttcagt taattcttct aacgattcgc aggcttcata acgcttgtag aattctatct
     1441 tctgtttttc atctgcaatg taactatctg gaatgaaaat atttgttttt acatatacag
     1501 gggttctgaa tacgtgtacc ggcttttctc ctttgagctt tcgcacagca tcctcaagca
     1561 tctggcagta tagatcaaag ccaacatcca tgatattgcc cgattgttct ttgcctaata
     1621 tattgcctga accgcgtatt tccatatcct tcatggcaat cttgaaacca ctgccaattt
     1681 cagtatattc tgcaattacc tgcaagcgct tctgtgcatc ttcagtaatg tgggtatccc
     1741 tgggataaaa gagatatgca tacgcctgcc tgtccgacct tccaacacgc ccctttaact
     1801 gatataactg tgataatcca aatgtatcag ccctgtttac aatgatggta ttgacattgg
     1861 gcatatcaag ccccgactca ataatagatg ttgaaaccag cacatcatat ttgccgttaa
     1921 taaagtcaat catgacttct tcaagctcgt gttcatgcat tctgccgtgt gccacacaaa
     1981 acgttgcttc gggcacaagc ttttctaagt actgggcctg ctcataaatg gtgctgatgc
     2041 ggttgtgcac atagaacacc tgacccttgc gctctatctc ttttaagata gccatgcgaa
     2101 caatatcagg attgtcttca ataaccagtg tttcaatgga ctggcggttt tctggtggtg
     2161 ttgcaataat tgacagatca cggatgccag caagtgccat gtgnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnngcaaaac gcttgcaaaa ggttgtatag tgctgcattg
     2341 ccagtactgt tgtgggaaca agtattgcaa cctgttttcc tgccattacc gctttgaatg
     2401 cagcccggat agccacctcg gttttgccaa atccaacatc gccacacacc aggcggtcca
     2461 ttggctgaga tttttccatg tcatctttta catcttcaat ggctgtgatc tggtcagggg
     2521 tttcttcata ttcaaacttt gattcaaatt cttcctgcca catggtgtct gcgggatact
     2581 gaaatccctt taatgctttt cgctgtgagt aaatttttaa taaatcctgt gctatttctt
     2641 ccactgattg ctgaacacgc tctttaatgc ggttccatgc agatttttta ccaagtgcat
     2701 caatacgtgg ttgacggcca tcaagcccca catatcgctg taccatattt atctggtcaa
     2761 gtgaaacgta cagcttatca ccatcagcat actctatgac taaaaagtca cgctcaacgc
     2821 caccagcact catgcgttca atggctttga aaatccctat gccatggtta atatgcacaa
     2881 catagtcgcc aggcgatagt tctaagaaag tttctattgg ctttgaatat ttctgtttga
     2941 aatgcttttt ctttcggtac gatttgccaa aaatttcgtg gtcggtgatg atacatgtct
     3001 tgatgcttcc tatctcaagg ccttcatgaa gatttgaaat aataatgcca aaatgctttt
     3061 tggggttata tgtatcaaaa tgctcctgtg gtttaaattc attgaataaa tcgtacagcc
     3121 tgcgtgcctg cccttcaaac gatgtgctaa caacaatatg ccatccttct tcaatgcgtt
     3181 ttgcgcactc attgcgtaca tcctgaatcc ttccctggaa tgatggtaat ccttttaagt
     3241 gccatacgtt cttggaatcg gttatgaatg ggtgcagttt gacagcctgc gattcaatgt
     3301 gctggtaact atcgcttagc aataattgcg atgctggaat gcagaaggtg ctgtcttttt
     3361 tgtgattgta caattgatgg taggttcgct caatttcaga caattttgct ttcagctgtg
     3421 gtggatcgca cagaaatatg tgtggattgt gcgcaaatga ggttattgta tcggtttgaa
     3481 ttgccagtgg gaacagatcg tatatgccag gtacatttgc tttttgcaat gactccactg
     3541 cctgctgatt acggttacta tcgccacgca gcaattcttt ttgtattttt tctttttcag
     3601 aatgagacaa caaaatttca cggcgtggat aaatggttat ggtttctacc tcaccaagtg
     3661 atacctgcgt catagggtca aagtagcgaa tggactcaca ggtgtcgcca aaaaaatcta
     3721 atcggacagg attggctgca ttgggaagat agatatcgat gataccacct ttaacagaaa
     3781 attgtccgta tcgatcaacg cgtgtttcgc gttcatagcc atagttgatg agtgtttcaa
     3841 tgattacttc aaatggatat tcttgatttc gttcaatgtg gatgcctttt tttaaaaaaa
     3901 attctttatg agggatagct cgtagcagtg attcaatggt tgcaaccaca atgacaggtt
     3961 ttcctgaaag aagctggtac agcgcagtaa tacgatcacg ttcaatagat gatgatgggt
     4021 taacatattc atacgggagt acttctaagg gtggaaaagg gaaaatggtg tcttcattga
     4081 caaatgctga tatgtcggca acaaagcggt tcattgtgtg atagtcggtg gtaaccacaa
     4141 tggtttgcgt ttgcagggtg ttaaatgatg ttgccacaat aaatggaaac gcagatgggt
     4201 gaatgccttc tatggaaaag gcttttacgc ccgaggctgc cagcgatttg aattcatcgg
     4261 ttgatgaata tatgttggta agttctttca ggaccatgat agtaagtagt aatgtgtata
     4321 ttgtgtatac tatgagtatg gtataaatta atttaatagt atgtatcaat aggcacgtgc
     4381 gtatgtatac tattcataaa tcaattgtac ttttgtttta gggatttttc aatctttttg
     4441 tgcggctctg accccatttt atttaattac attttcgata acattcttat tatgataata
     4501 tttcacacca taataaatgc ctgcagttat tccaataaca cctgaggtta tggctactgc
     4561 agatatgatg gtggtaacaa taccaaaatt tgttaaataa tacactgcta cacctgtacc
     4621 aagagaaata gttccgcttg atgaaaagaa taaagcatta tagccataat gaagtttatt
     4681 ctgacgactc tggcttaaat aataaataag gctggtttta tctaaagaac caactggatc
     4741 aggtttcgtt gtatccacag taaaggtgcc ttccacagga tactttttat gatgataata
     4801 ccgtttgttt tcaggtttat tgttatgtaa aagttttatg gtatatgtgt atgtatcagg
     4861 ggtcagagca atgggtattt gtactacgca gtacatcccg tctattattt caggatacag
     4921 agtatattcg ttgctctgac cctgtgaatt ggtgatagta accataacca taaaaggcct
     4981 attgctggta ttatatatat cgcaccatat aatgtaaggt cgtgtgtttg atatatcacc
     5041 tgcaggatac tgcaatggtt gggcgctttc attaatctgg gcaaatgaat acgtaaagca
     5101 ggttaaaagc attaatatac ttattgtaag tcgcataggt ttaccttgac aagagtgata
     5161 gtatactcta cagtgtcaga gcaatcaaat tatgcaacaa taaaattaat agagttaacc
//
LOCUS       DAORYL010000131         5103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_11122_length_5103_cov_6.082211, whole genome shotgun sequence.
ACCESSION   DAORYL010000131 DAORYL010000000
VERSION     DAORYL010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11122_length_5103_cov_6.082211"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..1435
                     /locus_tag="PLV81_14580"
     CDS             245..1435
                     /locus_tag="PLV81_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOM11364.1"
                     /translation="MKLHLVKSHSNIENHIADNVKTLPPSGIREFFDIVYNTPDCISL
                     GVGEPDFVTPWRISDSGIYAIKDGNTHYTSNKGLLELRQAIAAHIKKNCGIEYNPETQ
                     VLVTIGVSEGLDIALRSIVNPGDEIIYFEPCYVSYAATIKLSYGKAVAIATHFKDGFA
                     IDIDALKKAITSKTKAIMLNYPSNPTGASISKENLEAIAAIAIANNLIVISDEIYDAI
                     TYNGEHVSIISIPGMAERTIYLNGFSKAYAMTGWRLGYACGPEPIISAMNKIHQYTAL
                     CAPSIAQYAALEALQRGQRDVASMRSQYLKRRNFITARLNEMGLPCLPPKGAFYAFPD
                     ISPTGLSSRDFALRLLKSHKVAVVPGSAFGSYYDNHIRCAYATSMEQLKEAMDRMENF
                     VKGL"
     gene            1644..2348
                     /locus_tag="PLV81_14585"
     CDS             1644..2348
                     /locus_tag="PLV81_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11365.1"
                     /translation="MKLFKIIIMIFTMVISSSLFASDLGIGIAGWYADWEMYNPDNPN
                     NKITKMNSAFYIGPSIAYQFAEKWSTTLVTLFTPSPYKMTNDSGETTEITRYDADVAL
                     NYQLVRYLKFFVGGKYLGFTYNDGKHHGAGPGGGIGLTIPIVNNFYFLGNASGLYLWG
                     SHKDNSGTKDFTEYGYNAAAQLAFYAASAGLTTSIGYRYQFIKSKYNTDDPYGKKDEH
                     TFKGFTVLIVKSFHWE"
     gene            2899..3546
                     /locus_tag="PLV81_14590"
     CDS             2899..3546
                     /locus_tag="PLV81_14590"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HOM11366.1"
                     /translation="MFVDNSKLFTDYVVWAKFLLNHLKIPTEHSYVNLQCISQILAYK
                     LPQHYQIIKAYIDEALETLEKPFSDSPYIVEDNPYKDMAQEYLQFLLTTDRASASTLI
                     MQAVKNDVSIKDIYIHIFEPVLKEVGRLWQIHKITVAQEHYCTASTQLIMAQLYPFFF
                     SSSNKHGFSLVAACVNQELHEVGIRMVADILEMDGWMEYLLFGSKCSTTGYYRIC"
     gene            3503..3781
                     /locus_tag="PLV81_14595"
     CDS             3503..3781
                     /locus_tag="PLV81_14595"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOM11367.1"
                     /translation="MGANVPQQDIIEFVKEKKPHIVALSCTMTFHLHKIIETIKALRT
                     IADTTPIYIIVGGYPFNIDTNLWKKVDADLYAPNTIKISESLSKIFIS"
     gene            3791..4762
                     /locus_tag="PLV81_14600"
     CDS             3791..4762
                     /locus_tag="PLV81_14600"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOM11368.1"
                     /translation="MNTFQLIHSILTINEQGTIIKKHYSHALFEYNNLKDALDIGSID
                     YFNTFMQTVLSQGYAIGHSISFNVNNQYIKLILNGIQRNDVIHIFMLYDFAEAINAEL
                     QTINNELINTLRNTIKNFSDWNVGEPIFENKLFEEMAQLNNQLINMQRELVKKNIEIK
                     NLNEKLQQIAITDPLTGAFNRRYFYQKISEEIARAKRNNYSISLLYIDLNNFKTINDT
                     YGHEQDDRILTEFTKVVKFNIRNNIDSLFRFGGDEFVILLIDCTEEYANKVIERINKE
                     VSKIHNLLSMAYGISQIAPDKENIDIEAILVATDKKMYNHKKAMKMH"
     gene            4918..>5103
                     /locus_tag="PLV81_14605"
     CDS             4918..>5103
                     /locus_tag="PLV81_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11369.1"
                     /translation="MKLFKIIILIFTMVISSSLFASDLGIGIAGWYADWKMERKSSTE
                     GGTVNMDEVLYIGPSIAY"
BASE COUNT         1737 a          923 c          907 g         1536 t
ORIGIN      
        1 agatttctca cccctacggg gcttcgaaat gacaatgtga gtagtaaggg attcgtaatg
       61 acagtgataa catcgtcttt ttgacgaaca ctttaacatc gtcatttcga cgaacgcagt
      121 gaggagaaat cttaatactc agagaagaaa gatttctcac ccctacgggg cttcgaaatg
      181 acaatgtgag tagtaaggga ttcgaaatga cagcaacatt aattctatta ttacggagct
      241 attcatgaaa ttgcacttag ttaaatcaca cagtaatata gaaaatcata tcgcagataa
      301 tgttaagact ctgccacctt ccggcatccg tgaatttttt gatattgtat acaacacccc
      361 cgattgtatc tcattaggtg tgggagaacc agattttgtt acaccatggc gcatctctga
      421 ttcaggcatc tatgccataa aagatggtaa cacccattac acatccaaca aaggtttgct
      481 tgagcttcgg caggcaattg cagcacacat caaaaaaaat tgtggtattg aatataaccc
      541 ggaaactcag gtgcttgtaa ccattggtgt tagcgaaggc cttgacattg cgcttcgcag
      601 cattgttaat cccggtgatg aaattatcta ctttgagccc tgctatgttt cttatgcagc
      661 aaccattaaa ttgtcgtatg gaaaagcggt tgctatcgcc acccatttta aagacggatt
      721 tgccattgac attgatgcac tcaaaaaagc tattaccagc aaaacaaagg ccattatgct
      781 gaactaccct tcaaacccaa ccggtgcatc tattagcaaa gaaaatttag aggcaatagc
      841 tgccattgct attgccaaca atttaatcgt tatttcggat gaaatttacg atgccattac
      901 gtacaatggg gaacacgttt caatcatatc catacccgga atggccgaac gcacaattta
      961 tttaaacggc ttttccaaag cgtatgccat gaccggatgg cgcttaggtt atgcctgcgg
     1021 tcctgaacca atcatcagcg caatgaacaa gattcaccag tacactgcac tgtgcgcccc
     1081 atccattgca cagtatgctg cactggaagc cttgcaaagg ggtcagcgcg atgttgcatc
     1141 catgcgttca cagtatctga aacgccgaaa ctttatcaca gcacgcttaa acgagatggg
     1201 gctgccatgc ctgcctccta aaggtgcttt ttatgcattc cctgatatat ccccaacagg
     1261 attatcttca cgtgactttg ctctccggct tttgaaaagc cataaggttg cggtagtgcc
     1321 ggggagtgcg tttggaagct attatgataa ccacatccgc tgcgcctacg caacatccat
     1381 ggagcagctc aaagaagcca tggaccgcat ggaaaatttt gtgaagggat tgtaggtact
     1441 cttcaccaaa gcttgacaga aatgtacaga taaaaaagta atataaaatt gcattgacat
     1501 ttttatacaa accatctatt cattaagtaa taaagaatga ccataatgct aataagatag
     1561 tagcttattt ttcacattgc gggtttgcca taaacatgca ctcattaaca tttctttaga
     1621 ccacacatag ctggaggaaa cctatgaaat tgtttaaaat tattatcatg atttttacaa
     1681 tggttatatc ttcatcactt tttgcatcag atttaggcat tggaatagcc ggctggtatg
     1741 ccgactggga gatgtacaat cctgacaacc caaataataa aataacaaaa atgaattctg
     1801 ctttttatat tggcccttct attgcatatc agtttgcgga aaaatggagt actacccttg
     1861 tgactctctt cactccatca ccatataaaa tgaccaatga cagtggcgaa acaacagaaa
     1921 tcacacgtta tgatgccgac gtagctttaa actaccaatt agtacgatac cttaaatttt
     1981 tcgttggtgg taaatattta ggttttacat ataatgatgg caagcatcat ggtgcaggcc
     2041 ccggtggtgg tattgggcta actattccca ttgttaacaa tttttatttt cttggcaatg
     2101 cttcaggcct gtatctatgg ggaagccaca aagataatag tggcacaaaa gattttactg
     2161 aatatggata caatgcagca gcacagttag cattttatgc tgcgtcagca ggtttaacca
     2221 cctcaattgg ctaccgctat caatttataa aatctaaata taatactgat gacccttacg
     2281 ggaaaaagga tgagcataca tttaaagggt ttactgtact catagtaaaa tcgtttcatt
     2341 gggaatagat gtggaaatat ttttgtagcg atagttaccc acattcacag gcgattaata
     2401 aattgtatga taatgagttt tactttcatt ttgccaaaga ttttcccatc gtcattttga
     2461 cgaatgcagt gaggagaaat cttattcaca agcaaagatt tctcatccct tcgggattcg
     2521 aaatgacagt gctaacatcg tcatttcgac gaacgttttc ccattgtcat tttgacgaac
     2581 gcagtgagga gaaatcttat tcacaagcaa agatttctca tcccttcagg gttcaaaatg
     2641 acatatcagg atttttcatt acgatgaaat cgagatgaaa aataacatca tagttaaatt
     2701 ttttaaggga gggtaaattc aatgctatca aatgattatg tattttcata tattacccag
     2761 aatgcatcat cattggcaca ggatatttta gaacttcagt attcattaca acctgaactt
     2821 aaacaacact attcattata gcagcacaat aagtgcatta atgatatcat ataccatctc
     2881 aattatttag ccgaagcatt gtttgttgat aatagtaagc tttttactga ttatgttgta
     2941 tgggcaaaat ttttacttaa tcatttaaaa atacctactg agcattcgta tgtaaacctg
     3001 caatgtatca gtcaaatact ggcatataaa cttccccaac attaccagat aataaaagca
     3061 tatattgatg aagcacttga aacattagaa aaaccttttt cagatagccc ctatattgtt
     3121 gaagataatc cttacaaaga tatggcacag gaatatttgc aatttttact aactactgac
     3181 agagccagtg ccagtacact cattatgcag gcagtaaaaa atgatgtgag cattaaagat
     3241 atttatatcc acatttttga accggtattg aaagaagtgg gaaggttatg gcaaatccat
     3301 aagattaccg ttgcacagga acattattgc actgcatcaa cacaacttat tatggcacaa
     3361 ttatatcctt ttttcttttc atcaagtaat aaacatggtt tttcactggt agccgcatgt
     3421 gttaatcaag aactgcatga agttggcata cgaatggttg ctgatatatt agaaatggat
     3481 ggatggatgg aatacctact atttgggagc aaatgttcca caacaggata ttatagaatt
     3541 tgttaaagaa aaaaagccac atatagtagc attaagctgc actatgacat ttcatctcca
     3601 caaaattatt gaaacaatta aagcattgcg tactattgca gatacaactc ctatatatat
     3661 tattgttgga gggtatccat tcaatattga tactaattta tggaaaaaag tagatgctga
     3721 tttatatgca ccaaacacca ttaaaatatc agaatcactt tcaaaaattt ttattagctg
     3781 atatactgcc atgaatactt ttcaacttat acattcaata ctgaccatta acgaacaggg
     3841 gactattata aaaaaacatt actctcacgc attgtttgaa tataacaatt taaaagatgc
     3901 ccttgatata ggaagtattg attactttaa tacgttcatg caaactgtgc tgagccaagg
     3961 atatgccatt ggtcacagta tatcttttaa tgttaacaac cagtatataa agctcatact
     4021 taatgggata cagagaaatg atgtaataca catttttatg ttatacgatt ttgctgaagc
     4081 tattaatgct gaattgcaaa caataaataa cgagttaata aatactttgc gcaacactat
     4141 taaaaatttt tctgattgga acgtgggtga acctatattt gaaaacaaat tatttgaaga
     4201 aatggcacaa ttaaataatc agctaatcaa tatgcagcgt gaactggtta aaaaaaatat
     4261 agaaataaaa aacctgaatg aaaaactcca gcaaattgct ataacagacc cactaacagg
     4321 tgcatttaat agaagatatt tttatcaaaa aattagtgaa gaaattgcta gggcaaaacg
     4381 aaacaattat tcaataagtc ttttatacat agatcttaat aattttaaaa caattaatga
     4441 cacctatggt catgaacaag atgacagaat cctaacagaa tttacaaaag tagtaaaatt
     4501 taatatacgg aataatattg attcactttt taggtttggt ggggatgaat ttgttatact
     4561 gcttattgac tgtacagaag aatatgcaaa caaggttatt gaacgtatta ataaagaagt
     4621 atcaaagatt cataacctgc tcagcatggc atacggcata agccaaatag cacctgataa
     4681 agaaaatatt gatattgaag ccatactggt agcaacagac aagaagatgt acaaccataa
     4741 aaaggctatg aaaatgcatt gacatttttc tataaaccat ctattaattg atagtacaat
     4801 aattaaaact gacttcataa tggtaatggg atagtagctg ttattttata attcaggttt
     4861 gccataaaca tgcactcatt atcattgctt tagaccacat atagttggag gaaacctatg
     4921 aaattgttta aaattattat cctgattttt acaatggtta tatcttcatc cctttttgca
     4981 tcagatttag gcattggaat agccggctgg tatgccgact ggaagatgga aagaaaatcc
     5041 agcactgagg gtggcacagt gaatatggat gaagtactgt acattggtcc ttctattgca
     5101 tat
//
LOCUS       DAORYL010000132         4802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_12026_length_4802_cov_14.106804, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000132 DAORYL010000000
VERSION     DAORYL010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12026_length_4802_cov_14.106804"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(342..3485)
                     /locus_tag="PLV81_14610"
     CDS             complement(342..3485)
                     /locus_tag="PLV81_14610"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOM11370.1"
                     /translation="MKKSFLLLIVLCVFTSLAMAKDKPEMFVQLGHSHWVTSVAFSPD
                     GKYILSGSEDNTIKLWDAKTGIEIQTFTGHSDYVTSVVFSPDGRYVLSGSRDNTAKLW
                     EVETGKCIYTFSGHWFWVEAVAYSPDGNYILTASRDNTLKLFEVNTGKEIYTLKGNSE
                     AIVSVAFSPDGKYIAGCGENKAIIVWDVQSGKKVKILKGHSGFVTAVAFSPDGKYIIS
                     GSDDKTIKLWELSTQKNITTFQGHQGGVTSVAWSPDGKYVLSGSEDKTVKLWEIATGN
                     EIYSMSGHLYWVTSVAFSPDSKHAISGSKDNTIKLWDIEIGNEILTFKGYSSVVTSLA
                     YYSERNYLAVGGEDKTIKLWDLKTGKKFKTFKGHSDYITSVVFSPDGKYIITGSEDKT
                     LKMWDVATGKEIFTFGGHGGVVTSVAISPDGKYVVSGSEDKTIKLWDVLTGKEITTFV
                     GHKDYVSSVAFSSDGKYILSGSWDKTVILWEIESSKIVHVFSGHEFWVESVAFSPDGN
                     YVISGSKDNTIKLWDRISGKLIATYEGHTGDVTSVLFSKDGKLLLSGSKDTTLRLWAI
                     DTGKEIQTFKGHLSSIQSVSFDATGKYVFSGSWDGTVRQWDVISGKEIAQFISFNDDE
                     WIIITPEGYYTASSAGDKYLDVRIGNTVYSIENYRESFYRPDLVKLAIIGSSLKEHKT
                     IAEVKKPPVVEIVNTPKKVDSDEVQITVKLIDMGGGVGDIRIYLNDTSVILDNGRGIQ
                     ITEQMNDKELYRKYTIKLLTGQNVIRAIAFDKDGTVASNPVVCKVFSTYKPYTRPSLY
                     AIVIGINEYKNQKLELKYAAADAKLFSQTLKESSAPLFENVSIKLLIKKEETTKEHII
                     SALKEFNNLNPEDVFVFYVASHGIVDDGEYFLVTSNVGSLSTAKLKEDAISQAILKEL
                     IANIPSTKKLIVIDTCNAAKLSEALQVAFLTRGMSEETAMKILSRAVGSTILSASTSQ
                     QEALEGYQGHGLFTHVLVEGLKGKADYDKDGFIKTLELANYVDDQVPILAEKIFKRSQ
                     YPTATPHGQAFPVGKVK"
     gene            3792..4721
                     /locus_tag="PLV81_14615"
     CDS             3792..4721
                     /locus_tag="PLV81_14615"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOM11371.1"
                     /translation="MNDTKLWIALSRIEGVGIASLKAIHEALSNLHLSLYDIVELDPK
                     AIAHETGLSIELSQLIAKIAVTIEKIESEYEDLIDRNIEPVLFFDSRYPEKILKNVDY
                     PPPVLYYMGNTDIIKSRKVAILSDHNASERGYHIAFHAAAIAAQHSVTVVSGLAKGPQ
                     TMAHRGAVEAGGNTIAVLPMGMNSLTIPDVLKDTVNMSQWLFISPFNPQEDYALHNSY
                     KRNSLIAAMVDALIIIETGNSAGSFEAAKTACKYNTPLFVVEYAQYPQSASGNPVLIK
                     EYRATPLRGRIQNDMLVPNMDACIAKIKWGDSN"
BASE COUNT         1504 a         1005 c          706 g         1587 t
ORIGIN      
        1 cttctttttt aactgtaacc atgtcttcct tgaaagacca ggcatcatca aattgtggtt
       61 ttaccaacca tataatattg tttatattgc tcttttgtgg atacaccagc gtcaagaaac
      121 acaggataac tgcaataact aataatccct tgtacttgaa tttcatgtgc attacctcaa
      181 tagcaaaata atatagcaaa ataaaaatat attaccctac ccagttatat aaataactgg
      241 atatggatat catcacgcaa aaggaaattg caagcataat ttgctatttt ttaccatctc
      301 acaggcaaat taaggatgaa ctacaataca aattaaaaaa tttatttcac ttttccaaca
      361 gggaatgcct gcccatgtgg cgtagcagta gggtattggg atcttttgaa aattttctct
      421 gccaatattg gaacctgatc atcaacatag tttgccagtt ccagagtttt aataaatcca
      481 tctttatcat aatcagcttt accttttaag ccttccacaa gaacatgggt aaaaagacca
      541 tgtccctgat acccttccaa cgcctcctgc tgtgaggtag atgctgataa tattgttgaa
      601 ccaactgctc tggaaagtat cttcatggct gtttcttcac tcatcccacg tgtcagaaag
      661 gccacctgta atgcttcact taacttagcc gcattacagg tatctattac tattaatttt
      721 ttagttgaag gtatgttagc aattaactct tttaatatag cctgactgat tgcatcttct
      781 tttaatttag ctgtacttag tgaaccgacg ttggatgtta ctaaaaaata ttctccatca
      841 tcaactatac cgtgactggc aacataaaat acaaatacat cttctggatt taaattgtta
      901 aactctttaa gggctgaaat aatatgttct ttggtagtct cttctttttt aattaaaagt
      961 tttattgaaa cattttcaaa caatggtgct gaactttctt ttaaagtttg agaaaaaagt
     1021 tttgcatcag ctgcagcata ttttaattcc agtttttgat tcttatattc atttatacca
     1081 attactattg catacagtga tggtcgggta taaggtttat atgttgaaaa tactttacac
     1141 acaacaggat tgcttgcgac agtaccatct ttatcaaatg ctattgccct gataacattt
     1201 tgacctgtaa gaagtttgat agtatatttt ctatataatt ctttatcatt catttgttct
     1261 gtaatttgaa ttcctcttcc attatccaga ataacagatg tgtcattgag ataaattctg
     1321 atatcaccta caccacctcc catatcaata agttttacag ttatctgaac ttcatcacta
     1381 tcaacctttt tgggtgtatt tacaatttcc actacaggtg gtttttttac ttcagcaatt
     1441 gttttatgtt ctttaagaga actacctata attgctagct taacaagatc aggcctgtaa
     1501 aaggattccc tgtaattttc tatgctatat acagtatttc caatacgaac atctaaatat
     1561 ttatctcctg cacttgaagc agtataatac ccttcaggtg taataataat ccattcatca
     1621 tcattaaagc taataaattg agctatctcc tttcccgaaa ttacatccca ctgtcgtaca
     1681 gtcccatccc aactacccga aaaaacatat ttacctgttg catcaaaaga aaccgattgt
     1741 attgaactca aatgcccttt aaatgtttgg atttctttgc cagtatctat cgcccagaga
     1801 cgtaacgttg tatccttgct tccagaaagt aataatttgc catctttgct aaataataca
     1861 ctcgtaacat cacctgtatg gccttcatat gttgctatca gttttccact aatacgatcc
     1921 cacaacttaa tggtattgtc tttgctacca gaaattacat agttcccatc aggactaaat
     1981 gctactgatt caacccaaaa ctcatgtcca gaaaaaacgt gaactatctt tgaagattct
     2041 atttcccata aaatcacagt tttatcccag cttcctgata gtatatattt accatcagaa
     2101 ctaaatgcta ctgaagaaac ataatcttta tgcccaacaa atgtagttat ttctttccct
     2161 gtcaatacat cccaaagttt tatagtttta tcttcgcttc ctgacactac atatttgcca
     2221 tcagggctaa ttgctacaga ggttactaca cctccatgtc cgccaaaggt gaatatttct
     2281 tttccagttg ctacatccca cattttcaat gttttatctt cgctgccagt aatgatatat
     2341 ttcccatcag gactaaatac tactgatgta atataatcag aatgaccctt gaacgtttta
     2401 aattttttcc ctgtttttaa atcccatagt ttaatagttt tatcttcgcc accaacagct
     2461 aaataatttc tttcagaata atacgccaat gatgtgacta cacttgagta acctttaaac
     2521 gttaaaattt catttcctat ctcaatatcc cataatttga ttgtattatc tttactccca
     2581 gaaatagcat gcttgctatc aggactaaac gcaaccgatg ttacccagta aaggtgaccg
     2641 gacatactat agatttcatt tccagttgct atctcccaaa gctttactgt tttatcttca
     2701 cttcccgata gtacatactt accatccggg ctccacgcta ctgatgtaac acccccttga
     2761 tgtccttgga atgttgttat atttttttgc gtactcaatt cccataattt aatagtttta
     2821 tcatcactac cagaaataat atattttcca tcagggctaa aagccactgc agttacaaac
     2881 ccagaatgtc cttttaaaat tttaactttt ttccccgact gaacatccca tactataata
     2941 gctttgtttt caccacatcc cgcaatatac ttcccatcag gactgaatgc aactgataca
     3001 attgcttctg aatttccttt taatgtatat atttcttttc cagtgttcac ttcgaatagt
     3061 ttgagagtat tgtctctgct tgctgtcagg atataattac catcagggct atatgctact
     3121 gcttcaaccc aaaaccagtg tccagaaaat gtataaatac attttcccgt ttcaacttcc
     3181 cataatttag ctgtattatc acgacttcca gataaaacat atcttccatc aggactaaat
     3241 accactgaag ttacataatc tgagtgccct gtaaatgttt gtatttcaat tccagttttt
     3301 gcatcccaca atttaattgt attatcttca ctacctgaaa gaatgtattt cccatcaggg
     3361 ctaaatgcta ctgaagttac ccagtgagag tgtcccaact gaacaaacat ctcaggttta
     3421 tcttttgcca ttgccagaga agtaaaaacg cacaatacaa tcaaaagtaa aaatgacttt
     3481 ttcacattat tcccccatat gtttttcact aaactactga cctattatgc tgttatataa
     3541 aaattgcaag aattttatca gccaaaatat ttacaaaact gcaacttcaa atgtgaagta
     3601 taccattgaa tgtttgagta gaaccttttg tcacaatcac tacaaataac attttttagt
     3661 tatgtaaata atatctggaa taataattgg acgtattgaa cgttaaccat tatgcacaca
     3721 taaaatttag ttgatttaat acatcataca atcacaatcg ctcagaatat tggataatac
     3781 tatacaacgc tatgaacgat acaaaattat ggattgcact atcacgcatt gaaggtgtag
     3841 ggattgcttc tcttaaggca attcatgaag cactctctaa tctacatcta tcactatatg
     3901 atattgttga gctagatcca aaggcaatag ctcatgaaac aggcctatca atagaattaa
     3961 gccagcttat tgcaaaaatt gcagtaacta tcgaaaaaat tgaaagcgag tatgaagatt
     4021 taattgatag aaacattgag ccagtccttt tttttgacag ccgctatcct gaaaaaatac
     4081 ttaagaatgt tgattaccca ccacctgtgt tgtattacat gggcaatacc gatatcatta
     4141 aatcaagaaa ggttgcaata ttatccgacc acaacgccag tgagcgggga tatcatattg
     4201 cgtttcatgc cgcagctatt gccgctcagc attctgtcac tgtagtaagc ggccttgcaa
     4261 aaggtcccca aaccatggca catcgtggtg ctgtagaagc gggtggcaac accattgcgg
     4321 tcctgcccat gggaatgaat tcacttacca taccggatgt attgaaagat accgttaata
     4381 tgtcccagtg gctttttatt tcgccattca atcctcagga agactatgcc ctgcacaatt
     4441 catataaacg caacagcctg attgcagcga tggttgatgc actcatcatc attgaaacag
     4501 gaaacagtgc cggaagtttt gaagcggcaa aaactgcatg caaatacaat acccctttgt
     4561 ttgtggttga atatgcacag tatcctcaga gtgcctcagg caatcctgta cttataaaag
     4621 aatacagggc aactccctta cgtggccgca tccagaacga tatgctggtt cccaacatgg
     4681 atgcgtgtat tgcaaagatt aaatggggcg atagtaacta gacaataatt gttgacaacc
     4741 atcagctttt gccgatatta tataaggaga aatcttagtt tcacattgtc atttcgacga
     4801 ac
//
LOCUS       DAORYL010000133         4802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_12028_length_4802_cov_4.953866, whole genome shotgun sequence.
ACCESSION   DAORYL010000133 DAORYL010000000
VERSION     DAORYL010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12028_length_4802_cov_4.953866"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PLV81_14620"
     CDS             complement(<1..691)
                     /locus_tag="PLV81_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM11372.1"
                     /translation="MRNVYEKQFLDLEIDNARIYKEIANFYLQNGNYNASREYYSNIL
                     KVQPLNVEANFAMGNINEFLGYWKDAKGNYEICINAQPDFEYARKAHYELQKQHAPSI
                     TGNSIYFSDNIRTRYGIGLSGLYEPSQYFALQAGYELNKQTDSGGIATTGQLFYPSKG
                     NVTMHKAFAEIAVPFRFLYMRLFAHLGGTMYSGTVDYGNPLYDTSLSYMSLSYGAGFW
                     WMPPGTGITVKV"
     gene            complement(798..2420)
                     /locus_tag="PLV81_14625"
     CDS             complement(798..2420)
                     /locus_tag="PLV81_14625"
                     /inference="COORDINATES: protein motif:HMM:NF015038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOM11373.1"
                     /translation="MDIKIQIPEIPEEEQTPTVKALLNIISQCVQIIEMRDKEIQLLK
                     DEIARVKGRSPRPKIKASKLEEDIVKKAPRSRKDRQKKKKKSTTKYEEVVIKAENIPA
                     NSRFKGYKNYTIQNIEINSKTIVYRRECWKTLDGKYIIASLPDEVCGHYGAELKQYIL
                     HLNYDLNVPHELIVNSLHELGIKISAGQLNTILTEGHELFHEEKEALLKTGMEVSDYI
                     SVDDTGARHNGKNGYCTHIGNELFSYFKSTESKSRINFLRILRGRNTDYVLSEESFEY
                     MARQKLPSDKRKRLMGKLHQKFDTDDKWEEFLKITGIRSEKETTIVTEAALMGSILSH
                     GINKDLVILSDDAGQFEIFILLHALCWIHAERKLTYIVPINDYQKKIVDDMRGKIWDL
                     YQGLKNYKKSPAEDTKQFLEHQFDVIFSQTTEFEDINNALYLIRQNKKELLMVLNRPE
                     IPLHNNISENSIRIYVTKRKIHGGTRSEAGRNCRDTFMSLKKTCRKLNISFWEYILDR
                     LKKTYIIPPLHELLSRSLIKQSIFSPECASTF"
     gene            complement(2474..4699)
                     /locus_tag="PLV81_14630"
     CDS             complement(2474..4699)
                     /locus_tag="PLV81_14630"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM11374.1"
                     /translation="MIIKRRLKIFFTIISALVIAAITYAQFTDNAQSDEKNRTSKITY
                     KNYIWIEGENAVSTNFAREPIYNFFCSNKFALQLSKDVDPPQDGYFATYVFYVTHTKE
                     YDFWMGCTPPGSAYKDRPGYASPIEWQIDNGPFQTASAENVYVKSFYGIGGFYWVKIA
                     SGTLNAGKHTLTIRVKQKRSSGWDYYFYVDAILLLPQYSTGIAQLMDFPEVAPKDFAT
                     PGDGILFNAPEFYEKKLRANISDREALFSLVQIYGWLYDYNKAIFLLKKYLEKNPRDL
                     DMRLELAAFYSWSDQLDAAISEYKNIIAIDEKNITARKLLSVLAGWNNRYDEAIKYYQ
                     EIIAIDPENVDAYISLGTQYSWKGEMNRAIATFEKAETIAPSNIEVLEVLGDNYYWAG
                     RAYDAIRQYYRIIDINEKQISAYKKLAKIYLDLGESAKAARIINDAKQVVDLYPELSG
                     ISLDVKDELAKERRALIDSYKEALVKNPDDVNLRKGLVDTYIWNRMYGDAVKEYDALL
                     AYKMFHSIEQAEQKVTRLAIHTVGLQSLMPLIKTIKDDGSIIKKRYAKLIDDKKKGKA
                     VIEPSSLASDKATLEWYNAKLKEIQKELYVYNDITAAFGKDVQTYTAMQAATGWHFES
                     DRIYRYAATAKKLNPQMYQPYKVQGTIDLLYDKPSNAVSSFELVKKLAAGAEFPGLPL
                     AYAQTKKYNEAIALLEESLQSKKVSATYKNDIEQMLSLVKLSKGEVSSQSSLQVTTQK
                     K"
BASE COUNT         1344 a         1003 c          777 g         1678 t
ORIGIN      
        1 ccacttttac ggtgatacct gttccaggag gcatccacca gaacccagcc ccataggaca
       61 atgacatata acttagtgat gtatcataca aaggattgcc gtaatctacc gtacccgaat
      121 acatggtacc gcccagatgt gcaaaaagcc gcatatacaa aaacctgaag ggaactgcaa
      181 tttcagcaaa agctttgtgc attgtcacat tccctttaga tggataaaag agctgcccgg
      241 ttgtagcaat gcctccagaa tcagtttgtt tattcaattc atagcccgcc tgtaatgcaa
      301 aatactggct gggctcatat agaccactta agccaatacc atagcgtgtg cgtatattgt
      361 cactaaaata tatactattg ccagtaatag aaggagcatg ttgtttttgt aattcataat
      421 gagccttacg tgcatattca aaatccggct gtgcattaat gcatatttca tagtttccct
      481 tagcatcctt ccagtatccc aaaaattcat tgatattgcc catagcaaaa tttgcttcca
      541 cgttcaacgg ctgcactttt aaaatatttg aataatattc tcgtgaggca ttataattgc
      601 cgttttgcaa atagaagttg gctatttctt tataaatacg cgcattgtct atctccaggt
      661 ctaagaactg cttttcatac acattgcgca ttgtggcaag gccaaatgaa agcttttggt
      721 taacatcatc atcaatagat tccagtaatg agtctgtatt tgccagtaaa acttcaatat
      781 cctgaagtgg taactactca gaaagtagag gcacactcag gggaaaatat agattgtttg
      841 attaagcttc tacttaatag ttcatgtaaa ggtggaataa tataagtctt ttttaaacgg
      901 tccaaaatat attcccaaaa agaaatgtta agttttctac aggttttttt cagactcata
      961 aatgtatcac gacaattccg cccggcctca cttctggttc caccatgaat tttccgttta
     1021 gtaacatata tacgtatcga gttttcgctg atattattat gaagaggtat ttccgggcga
     1081 tttaatacca taagaagttc ttttttattc tgccgaatca gatataaagc attgttgata
     1141 tcttcaaatt cggtcgtttg agaaaaaatt acatcaaact gatgttccag gaactgcttt
     1201 gtatcttctg caggcgattt cttatagttt tttaatccct gatataaatc ccatatcttc
     1261 ccacgcatat catctacaat ctttttctga taatcattta taggcacaat ataggtaagt
     1321 tttctttcag cgtgaatcca gcataatgca tggagcaata taaaaatttc aaattgtccg
     1381 gcatcatcgc tcaggataac aagatcttta tttatcccgt gggaaaggat actccccatc
     1441 aatgcggctt cagtaacaat ggtagtttct ttttcacttc taatgccggt tatctttaaa
     1501 aattcttccc acttatcatc agtatcaaat ttctgatgca gctttcccat taagcgtttc
     1561 cttttgtctg acggaagctt ctgtcttgcc atatattcaa atgattcttc gctaagaaca
     1621 taatcagtat tacggccacg cagtattctt aaaaaattta tacggctttt gctttcagta
     1681 ctcttaaaat atgaaaatag ttcattccca atatgagtac aataaccgtt tttaccattg
     1741 tgacgcgctc ctgtatcgtc taccgatata taatcagata cttccattcc tgttttcaac
     1801 agcgcttcct tctcttcgtg aaacaattca tgcccttctg ttaatatggt gttcagctgc
     1861 cctgctgaaa tctttatgcc aagctcatgg agactgttta ctattaactc atggggtaca
     1921 ttcaaatcgt aatttaaatg cagaatgtac tgttttagtt cagctccgta atgtccgcaa
     1981 acctcatccg gtaatgatgc aataatatat ttaccatcca aggtcttcca acactctcga
     2041 cgatacacta ttgttttgga atttatctcg atgttctgaa tggtgtaatt tttatacccc
     2101 ttaaatcgcg aattggcggg tatgttttct gcctttatta ctacttcctc gtatttcgtg
     2161 gtagatttct ttttcttctt ctgcctatct ttccgtgacc ggggggcttt cttcactata
     2221 tcttcttcga gcttgctcgc tttaatcttt ggcctggggc ttctcccttt gacacgtgct
     2281 atctcatctt ttaataattg aatctcttta tcccgcattt caattatttg tacgcattga
     2341 ctgattatat tcagtaatgc tttaacagtt ggagtctgct cttcttccgg tatctccggt
     2401 atctgtatct ttatatccat taagacaaag taaaattaat ccattaaaat taatagtctt
     2461 tttttttaat tgcctacttt ttttgagtag ttacctgaag tgagctttga gaagatactt
     2521 ctcctttact taattttacc agtgatagca tttgttcaat atcattctta tatgttgcag
     2581 atactttttt actctgcaat gactcttcta aaagagctat cgcctcattg tatttttttg
     2641 tttgtgcata ggcaaggggc aacccgggaa attcagcgcc ggcagccaat ttttttacca
     2701 actcaaaaga actgactgca tttgacggtt tatcatacaa taaatcaatg gtaccctgaa
     2761 ccttatatgg ttgatacatt tgtggattta atttctttgc agttgcagca tacctataaa
     2821 tcctgtcaga ctcaaagtgc cagcctgttg ccgcctgcat tgctgtatac gtttgcacat
     2881 ctttgccaaa tgccgcagtg atatcattgt atacatacag ctctttctgt atttctttta
     2941 attttgcatt gtaccattca agcgttgctt tatcgctggc aagcgacgat ggctcaatga
     3001 cagccttgcc tttcttttta tcatctatca gttttgcata tcgtttttta attatgctac
     3061 catcatcctt aatggtttta atcaaaggca ttaatgattg caatccaaca gtatgtatgg
     3121 ccaatcgtgt taccttttgt tcggcctgct caattgagtg aaacatctta tatgcaagca
     3181 gagcatcata ttccttaacc gcatcaccat acattctgtt ccatatatac gtatcaacaa
     3241 gccctttgcg taaattgaca tcatcaggat tttttaccag tgcctcttta taactatcta
     3301 ttaatgcccg acgctctttt gcaagctcat ctttaacatc taatgatata cccgatagtt
     3361 ccggatataa atctacaacc tgttttgcat cattaattat tcttgctgct tttgcacttt
     3421 cacctaaatc aagatatatc ttagccaatt ttttatatgc tgatatctgc ttttcattta
     3481 tatcgattat gcggtaatat tgtcgtatgg catcatacgc ccttcctgcc cagtaataat
     3541 tatcacctaa aacctcaagc acttcaatat tactgggggc aatagtttca gccttttcaa
     3601 atgtggcgat agctctgttc atttcacctt tccacgaata ttgtgtaccc agtgaaatat
     3661 acgcatccac attttccgga tctatggcaa taatttcctg ataatacttt attgcttcat
     3721 catagcgatt gttccagcct gcaagtactg aaagaagctt tcgtgcagtg atattttttt
     3781 catcaatagc aatgatattt ttatattcac tgatggctgc atctaattga tctgaccatg
     3841 aataaaatgc agcaagctct agccgcatat caagatcacg tggatttttt tccaaatatt
     3901 tttttagtaa aaatattgct ttgttataat catatagcca accataaatt tgcacaaggg
     3961 aaaatagtgc ttctctatca ctaatatttg cacgtaattt tttttcataa aattcaggag
     4021 cattaaacag tatgccatca cccggagtgg caaaatcttt tggtgcaacc tcaggaaaat
     4081 ccataagctg tgctatacct gtactatatt gaggcaataa taaaatggca tcaacataaa
     4141 agtagtaatc ccagccgctg cttctttttt gttttactct aattgtcagg gtgtgtttcc
     4201 cagcattgag tgtaccactg gcaattttta cccagtagaa cccaccaata ccataaaaac
     4261 ttttcacata cacattctca gctgatgctg tctgaaacgg gccgttgtca atttgccatt
     4321 caatggggga tgcatagcca ggcctatcct tatatgctga tccgggaggg gtgcatccca
     4381 tccagaaatc atattccttg gtatgggtaa cataaaaaac ataggtagca aaataaccgt
     4441 cctgtggggg atcaacatct tttgaaagtt gtaacgcaaa cttattactg caaaagaagt
     4501 tatatatggg ttcacgggca aaattcgttg ataccgcgtt ttcaccttct atccatatat
     4561 aatttttata tgttatctta gaagtgcggt ttttttcatc gctttgagca ttatcggtaa
     4621 attgcgcata ggttattgct gctataacca gagcacttat tattgtaaaa aatatcttta
     4681 atcttctttt tataatcata tatacccctc atccccaaat ggtgtcttac tttactaatt
     4741 tttgaagtat cattattgcc tgcaatcctg ttactgattc atcaggatat gcatcctggc
     4801 tc
//
LOCUS       DAORYL010000134         4704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_12339_length_4704_cov_14.626801, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000134 DAORYL010000000
VERSION     DAORYL010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12339_length_4704_cov_14.626801"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PLV81_14635"
     CDS             <1..483
                     /locus_tag="PLV81_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HOM11375.1"
                     /translation="NRDGEVLDDPESFKTKEVSKLSVLDNIFQPDEYPALYDGYHHVV
                     RINYYPPRGDNKESWDNIDIRGWLDYPMQIKVNFLCRDSILAAPVVLDLVLFFDLAQR
                     ASMYGIQEWLSFYWKSPMFEPGEEPIHDLFVQLMKLKNTLRWLMDVPPVTYLGNETYD
                     "
     gene            541..2202
                     /locus_tag="PLV81_14640"
     CDS             541..2202
                     /locus_tag="PLV81_14640"
                     /inference="COORDINATES: protein motif:HMM:NF024707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HOM11376.1"
                     /translation="MEPTVLLLGINAKFIHSTLSLYYLKQIIASYDYHGTILETTINN
                     PVQETLTSIHAIQPDVCCIAVYIWNAQYIKRLLPLLHVTLPQCKIILGGPEVSYTSND
                     WLKQFPFVDCIIQGPGEKSFEYLANHQFNICEKILHCPNYHFNDIPFPYTSEDLKHLA
                     NHYIYYESSRGCPYSCSYCISSREDQKLQFKDIQKVYDELDVLLHHNVRMVKFVDRTF
                     NCNPTHSRALFTYLLDHNNDHTQFHFEIHPNLLYEEDFEILKWVPPQYFQFEIGIQSI
                     HENTLHAIQRKMNWEKARENIRKLVELQNIHIHLDMICGLPYEDMEGIKESFNAIVSL
                     KPHYFQMGFLKILPGTNIDTKKYEYGIEHEDNPPYMLKKNKWLSHEEMQLLHHIERVV
                     DSLYNHHFNTTSLMLADFIAQDNLFDLYHEIANFFQEHNFSLYAKSWESIAAILLEFI
                     KHSYPEYTKLATDCLRWDWFVKSNNKWVPPFIRSSSNPNTIKEMIIQQNRISKQELSL
                     NKTIVSINDIQRSQIFIAESDHFMQWRMNNHHYAVKINGTILLID"
     gene            complement(2203..2967)
                     /locus_tag="PLV81_14645"
     CDS             complement(2203..2967)
                     /locus_tag="PLV81_14645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOM11377.1"
                     /translation="MGSLSYNLDGKVAIVTGGTRGIGLAIAQELLRQNAKVVICARKQ
                     EGLDKAKEILKGGDNLVTCVAHIAKEEDVNSLVDTTVKAFSKIDILINNVGMNLPTGS
                     LVDVELSAWQKIIDSNLTGTFLVSKKVGAIMRQQKSGKIVSISSIAGRLASPGMNVYG
                     IAKAGIEMMTKNLALELAPYNVQVNAVAPGMVRTDFSKPFWSNDAIHEMIVKNIPIGR
                     IAEIDEVVHPVLFLASDGARYITGQTLLIDGGASIV"
     gene            complement(2989..>4704)
                     /locus_tag="PLV81_14650"
     CDS             complement(2989..>4704)
                     /locus_tag="PLV81_14650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOM11378.1"
                     /translation="VVPSIRSVQDRVSIQLDRGCKNLCKFCHAGYWELPYRKVPAKSI
                     ADTILDTVQHTGFSDVSLHSLSVGDYSDIVELLNYIVPPLVEQGISISLPSLRVDLQT
                     LDIIESISDLKKTSLTFAIESASDMLRYKAYKRLTVEEIMNIITVLSQRGWQRIKLYF
                     MIGLPGCEQVDEAADIVAFMRQACSINKKIQYNVTISPFVPKPHTPFQYEKQYDETYC
                     LEVIRTIKRSVPKSVTIKNHDVYASLIEGVFARGDSLLSNALVGAYHNGARLDLWGEH
                     FDFTIWDNALQKTAGNWRRYLEKRPSQELSPWHVIKTRYDSLIHKKMHSSWDGKTFPA
                     GFKKVKLNKDAFKEAARSFEKRYKAVATLRVKYAKKGKAQFLGHIDCMEIFKRALRMA
                     QVPVAYTQGYNKHERLVTGPALPLGYESQCEFVDVELFQQCSDTVVRNFGTLFPDGFE
                     IISAYHHTDTTLPLQSIAYSRYSITFLDGRLRDMFATKLKNKTDIVKKTKKGLKNLAF
                     DDVVVEFQIHDDSVELLIPVGEGSIRPDNLIADWCEVNNPLAVAAISKTAVYYMDNGA
                     FVEFI"
BASE COUNT         1432 a         1018 c          841 g         1413 t
ORIGIN      
        1 aaccgtgatg gcgaagttct tgatgaccct gaatcattca aaactaaaga agtaagtaaa
       61 ctttcagttc tggataatat tttccagcct gatgaatacc ctgcactgta tgacggctat
      121 caccatgttg tcagaattaa ctactaccca cctcgtggcg acaacaagga aagctgggac
      181 aacattgaca tacgcggctg gttagattat ccaatgcaga ttaaggtcaa tttcctgtgc
      241 cgcgactcaa tattagctgc tcctgttgtt cttgatcttg tactcttctt tgatctggct
      301 cagcgtgcat caatgtatgg tattcaggaa tggctctctt tctactggaa gagcccaatg
      361 tttgaacccg gcgaagagcc aatccatgac ctttttgtcc agctcatgaa attgaaaaat
      421 acattgcgat ggctgatgga tgttcctcca gtaacctact taggaaatga aacatatgac
      481 tgataataat aataaaaaga gggcttgcta aaagccctct tttagtatat acttcaatat
      541 atggaaccaa cggtactgtt actgggaatt aatgctaaat tcatccactc cactctatca
      601 ctttactatt taaaacaaat tattgcatca tacgattatc atggtactat ccttgaaact
      661 accattaata atccggtgca ggaaaccctt accagtatac acgccataca acctgatgta
      721 tgctgcatag ctgtatatat atggaatgca cagtatatta aaaggctttt acctttgtta
      781 catgttacac ttccacaatg taaaatcata ttaggtggtc ctgaggtaag ctacaccagt
      841 aatgactggc taaagcagtt cccgtttgtt gactgtatca tacagggacc cggtgaaaaa
      901 tcctttgaat atttagctaa ccaccaattt aacatatgtg aaaaaattct gcattgccca
      961 aattatcatt ttaatgatat cccttttccg tatacctctg aagatttgaa acatttagcc
     1021 aatcattata tttactatga atccagccgc ggatgtcctt attcatgcag ttactgtata
     1081 tcatcacgtg aagaccagaa attgcagttt aaagatatcc aaaaagtata tgatgaactt
     1141 gatgttcttt tgcatcataa cgtgcgtatg gtaaaatttg ttgatagaac ctttaactgt
     1201 aatcccacac attcacgtgc gctgtttaca tatttacttg atcataataa tgaccatact
     1261 caatttcatt ttgaaattca tcctaacctt ttatatgagg aagattttga aatattaaaa
     1321 tgggtgccac ctcagtattt ccagtttgaa attggaattc aatccataca tgaaaataca
     1381 ttgcatgcca ttcagcgaaa gatgaactgg gaaaaagcaa gggaaaatat tcgcaaactt
     1441 gtagaattac aaaatatcca catccatctt gatatgatat gcgggcttcc ctacgaagac
     1501 atggaaggga ttaaggaaag ttttaatgca atagtaagct taaagcctca ttacttccag
     1561 atgggatttt tgaagatact gcctggaaca aatattgata caaaaaaata tgagtacggc
     1621 attgaacatg aagacaatcc accttacatg ctgaaaaaaa ataaatggct ttcacacgag
     1681 gagatgcaac ttcttcacca cattgaacgc gttgttgata gtttatataa tcatcatttt
     1741 aacacaacat cgctaatgct tgctgacttc attgcacaag ataatctgtt tgatttatat
     1801 catgaaattg ctaacttttt tcaggaacat aacttttcat tgtatgcaaa aagctgggaa
     1861 tcaattgctg caatactgct tgaatttatt aaacacagct accctgaata tactaagctt
     1921 gccactgatt gcctgcggtg ggattggttt gttaaatcaa ataataaatg ggtgccacct
     1981 tttatacgaa gtagtagcaa cccgaatact ataaaagaaa tgattattca gcaaaacaga
     2041 atatctaagc aggaactatc gcttaataaa actatagttt ctattaatga catacaacgt
     2101 tcacagatct ttatagccga aagtgatcac tttatgcagt ggcgtatgaa caaccaccac
     2161 tatgcggtaa aaattaatgg tacgatactc ctgatagatt gatcacacta ttgaagcacc
     2221 accatcaatt aagagtgttt gcccggtaat atatcgcgct ccatctgatg ccaggaaaag
     2281 aacaggatgc acaacttcgt caatttcggc aattcttcct ataggtatat tttttactat
     2341 catttcatga atggcgtcat ttgaccagaa tggcttgctg aaatcagttc gcaccattcc
     2401 gggtgcaacc gcatttacct gtacattata tggtgcaagc tccagcgcca gattttttgt
     2461 catcatctca atgccggctt ttgcaatgcc gtacacattc atacctggtg aagctaacct
     2521 tcctgcaatt gatgatatgc tgacaatttt tccgctcttc tgctggcgca tgattgcacc
     2581 aaccttcttt gacacaagaa atgtgccggt aaggtttgaa tcaataatct tttgccatgc
     2641 cgatagttca acatcaacaa ggctccccgt tggcaggttc atgccaacat tattaattaa
     2701 tatatcaatt tttgaaaaag cttttactgt tgtgtccacc aggctattca catcctcttc
     2761 tttagcaata tgagcaacac aggttacaag gttatcgcca cccttaagga tttcttttgc
     2821 cttatccagc ccctcctgct tgcgtgcaca aatcactact tttgcattct ggcgtaacag
     2881 ctcctgggca atagcaagcc caataccacg agtgccaccg gttacaatcg caaccttgcc
     2941 atctaaatta taagaaagcg aacccataca aagcctcctt tttgaaagtt atatgaactc
     3001 aacaaaagct ccattatcca tataatatac agcagtttta ctaatagctg ccactgctag
     3061 tggattgttt acctcacacc agtcagcaat gagattatca ggccgtattg aaccttcacc
     3121 caccgggatg agcagttcaa cactgtcatc atgtatctga aactctacaa caacatcatc
     3181 aaaagctaaa ttcttcaatc ctttttttgt cttcttaact atatctgttt tgttctttaa
     3241 tttggtggca aacatatcac gcagcctgcc atcaagaaag gttatactat agcgggaata
     3301 tgcaatggat tgcaggggaa gtgttgtgtc ggtgtgatga taggctgata taatttcaaa
     3361 cccgtcagga aataatgttc caaaattacg tactactgta tcactgcatt gctgaaagag
     3421 ttcaacatca acaaactcac actggctttc atagcccagc ggcaatgcag gcccggtaac
     3481 cagacgttca tgtttattat atccctgcgt ataggcaact ggaacctggg ccatacgcag
     3541 tgctcgcttg aaaatttcca tgcaatctat gtgccctaaa aattgtgctt tccccttttt
     3601 agcatacttc acacgcaggg ttgcgacggc tttataccgt ttttcaaatg accgggcagc
     3661 ttctttaaat gcatctttat taagctttac ttttttaaaa ccagcaggaa atgttttgcc
     3721 atcccagctt gaatgcatct ttttgtgtat aagggaatcg tagcgtgttt taataacatg
     3781 ccagggcgat agttcctgtg atggcctttt ttctaaataa cggcgccagt taccagcagt
     3841 cttttgcaat gcattgtccc atatagtaaa atcaaaatgc tcaccccata aatccagtcg
     3901 agctccattg tggtatgctc caacaagggc atttgaaagt aaactatcgc cacgtgcaaa
     3961 cacgccttca attaaagaag catagacatc atgatttttt attgttaccg attttggtac
     4021 actgcgttta attgtgcgta tgacctcaag gcaataggtt tcatcatact gcttttcata
     4081 ctggaatggc gtatgcggtt ttggtacaaa cggcgagata gtaacattgt actgtatttt
     4141 tttattgatt gaacatgcct gcctcatgaa tgcaacaata tcggctgctt catctacctg
     4201 ctcacaacca ggcaggccaa tcataaagta tagctttatg cgctgccacc cacgctggga
     4261 caacacagtg ataatattca tgatttcttc aacggtaaga cgcttgtacg ccttataacg
     4321 cagcatatcc gaagctgact caatagcaaa agtcagcgat gtttttttca aatcagatat
     4381 tgattcaata atatcaagcg tttgtaaatc caccctcagc gaaggaagcg atatactaat
     4441 accctgctct accagtggcg gaacaatata attcaaaagc tccactatat cagaataatc
     4501 tcccacactc agtgagtgca gcgatacatc gctaaaccca gtatgctgca cggtatcaag
     4561 tattgtgtca gctattgatt tagcaggtac ctttcggtag ggaagctccc agtagcctgc
     4621 atggcaaaac ttgcacaggt tcttgcagcc tctgtctaac tgaatggaaa ccctgtcctg
     4681 cacactgcgt atggatggca ccac
//
LOCUS       DAORYL010000135         4653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_12517_length_4653_cov_7.094606, whole genome shotgun sequence.
ACCESSION   DAORYL010000135 DAORYL010000000
VERSION     DAORYL010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12517_length_4653_cov_7.094606"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..654)
                     /locus_tag="PLV81_14655"
     CDS             complement(196..654)
                     /locus_tag="PLV81_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11379.1"
                     /translation="MRKFKSVLIVLFCIANSITYSNAKDISYTLDDRDRLIRVEEGLK
                     GVNQRIDSIEKRIDGLDKRIDGLQGLMYVVIGAIIAQTLAVVGFSLWDRRSALMPVTR
                     KTKELEEYIESTLKETKEIKEREIVLENAIREYAKHEPKLYEVLKSLRLL"
     gene            complement(778..866)
                     /locus_tag="PLV81_14660"
     tRNA            complement(778..866)
                     /locus_tag="PLV81_14660"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(830..832),aa:Ser,seq:cga)
     gene            complement(998..1645)
                     /locus_tag="PLV81_14665"
     CDS             complement(998..1645)
                     /locus_tag="PLV81_14665"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOM11380.1"
                     /translation="MRIILLGAPGAGKGTIAKMLVEKYGIVQISTGDILRQAVKEGTP
                     VGKKAAEYMNTGKLVPDDLIMDIIEERIQRPDCAKGFIFDGFPRTIPQAEALGKLFAT
                     KNMTIDAIINLEVPEDVVLKRLTSRRTCSNPDCQAIYNIYTMPPKKDGICDKCGSKLV
                     QRDDETEEAIKVRLATYYEKTQPLIDYYKNSKNYFAVPSLIAQETFDEIVKRLGK"
     gene            complement(1700..2206)
                     /locus_tag="PLV81_14670"
     CDS             complement(1700..2206)
                     /locus_tag="PLV81_14670"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOM11381.1"
                     /translation="MAVEQTLIIIKPDGLVKSLVGNILTRLSEAKLIIVGAKALRPTR
                     ELAEKHYEHLKDKPFFEELIQYIMGELHKTYRVVAFVYEGEDAIKKVRDIVGDTNPEK
                     ANPVTIRGAYGRITTSGVFENVVHASSSPEDAEKEIKLWFEPDELVDVLYPVKEVTLE
                     KVTKKVWA"
     gene            complement(2347..2526)
                     /locus_tag="PLV81_14675"
     CDS             complement(2347..2526)
                     /locus_tag="PLV81_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11382.1"
                     /translation="MLEYNVKNEYELIAKHCPKCQALVMAAVVKTLQGEILKYKDIRC
                     SSCDWQSPLAQLKSS"
     gene            3231..4019
                     /locus_tag="PLV81_14680"
     CDS             3231..4019
                     /locus_tag="PLV81_14680"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOM11383.1"
                     /translation="MFLGKKALSIVLVLLLSFVFSCSSQDKAKTLRNTPRELRKGLLI
                     LNFYNSTPKSRASEFEPWEYGLASMLMTDMESIGVFNIISKEALKDVIAEQEFQQTGM
                     IDPKTLVKLGNLVGAQYILKGSFMEMNGMLRIETQVFSVEKGVQLQTAVVNGKTDTFF
                     TLEKELVDKITQIVDVVLTESEKNVILAKIETKSVEASLKNYKGELTVEEAKKLAQEG
                     KKEEAKKLIEQAKKDFKEAVAIDPNYEKAKKNLSKISMAIPVTL"
     gene            complement(4121..4459)
                     /locus_tag="PLV81_14685"
     CDS             complement(4121..4459)
                     /locus_tag="PLV81_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11384.1"
                     /translation="MGVLGILCIILLLFIVLLLMLKVNIFSKAFWPRKEVLNQHVHTL
                     YGLVGTFGVAYITDGFPYSAFAGLFAGLALEFLQYFVRGGKLHIEDRIRDVFFWTFGG
                     MIGYSFYIVK"
BASE COUNT         1453 a          910 c          722 g         1568 t
ORIGIN      
        1 tgcgctacac acggtttcca accgtgctct ttcggcctct cagacatctc tcctgaagtt
       61 tttgattgtt acgttattac acttttactt gctgtgtttt tattgtcaat aatctttaaa
      121 atatatgatg gcatttgcat ctttaaaaat ttgactatta acatctatat tatatttggc
      181 tagaaaaaga ctattttaca ataatcgtaa cgatttcagt acctcgtaca gctttggttc
      241 atgttttgca tattcacgta tagcattttc aagcacaatt tcacgttctt ttatttcttt
      301 tgtttctttc agagtagatt ctatatattc ttcaagttct tttgtttttc ttgttacagg
      361 cataagtgct gaccttctat cccataagga aaaaccaacc accgctaaag tttgtgcaat
      421 gatagcacca ataacgacat acattaatcc ctgcaaacca tcaattcttt tatccaaacc
      481 atctatcctc ttttctatac tgtcaatacg ttgattaaca cctttcaacc cttcttcaac
      541 tcttatcagc ctatctctat catccagagt ataagaaata tccttagcat tggaataagt
      601 tatactatta gcaatacaaa acaatacaat taatacactt ttgaattttc tcataattaa
      661 tcctttcctt atttaaccac tgggaaatat ttcttctcat accaggaatg ctaatgccta
      721 taatatacta tttgttatat actgtcaatt aattttcgtt atagaacaaa ttccattcgg
      781 agagagaggg attcgaaccc tcggtcccag tcacccggga caacggtttt cgagaccgcc
      841 cctttcaacc tctcaggcat ctctccatac attgtttata ttgtaggggc acattttgaa
      901 tgtgcttcta caataatata tttcatacac atattattac aaatatccca aacaattttt
      961 ataacaatta tatttgttat atattgtaaa gaaattttta tttgcccaat cgttttacaa
     1021 tttcatcaaa tgtttcctga gcaatcagtg aaggtactgc aaaatagttt ttactgttct
     1081 tgtaataatc aatcaggggt tgtgtttttt cataatatgt ggcaaggcgc acctttattg
     1141 cttcttcagt ttcatcatca cgctgcacca gtttactgcc gcatttgtca catataccat
     1201 ccttctttgg cggcatggta tagatattat aaattgcctg acaatcagga tttgagcagg
     1261 ttcttcggct ggtaagcctt tttaatacca catcctcggg cacttcaaga ttaatgatgg
     1321 cgtcaatagt catatttttt gtagcaaaca attttccaag tgcttcagcc tgaggtatgg
     1381 ttctgggaaa tccatcaaat atgaaacctt ttgcgcagtc aggtcgttga atgcgttctt
     1441 ctatgatatc catgatcaag tcatcgggaa ccagttttcc agtattcatg tactctgctg
     1501 ctttttttcc taccggggtg ccttccttca ctgcctgccg taaaatatca cctgtcgaaa
     1561 tctgaactat gccatatttt tcaacaagca ttttggcgat agttccttta ccagcccccg
     1621 gtgcacctaa aagaataatc cgcatataat aattcctccc caaaagtttt gatatgaaat
     1681 tgtgaattgc tatttcttct tacgcccata ccttctttgt gactttttcc agagtcactt
     1741 ccttaacagg ataaagaaca tcaacaagtt catcaggttc aaaccacaat ttaatttcct
     1801 tttcagcatc ttcagggctg gatgatgcat ggactacatt ttcaaacact ccagaggttg
     1861 taatacgccc atatgctccc cgtatagtta caggatttgc cttttcagga ttagtatcac
     1921 ctacaatatc ccttactttc tttattgcat cttcaccttc atatacaaac gcaacaactc
     1981 tgtaggtttt atgcaactcc cccataatgt actgaatcaa ctcttcaaaa aacggcttat
     2041 cttttaaatg ctcataatgc ttctcagcaa gctccctggt ggggcgtaaa gcttttgcac
     2101 caacaattat taattttgct tctgaaaggc gggtcaaaat atttcctact aaagacttaa
     2161 caagcccatc tggctttata attattaagg tttgctctac tgccatagtg ttacctcttt
     2221 cttatatttt agggatccca tcctctgtgt gtatgttaac actattagcc tttcagtgtc
     2281 aacaaattga ccatcagaag atagtataac tatggcagtg ctattactct gaatagcctt
     2341 attttatcaa gaacttttta gctgagctaa tgggctttgc caatcacagc ttgagcagcg
     2401 gatatcctta tatttcagga tttcaccctg caatgtttta actaccgctg ccatgaccag
     2461 agcctggcat ttggggcaat gcttggcaat gagctcatac tcattcttaa cattgtactc
     2521 cagcatggat acgcctccgt ttatttataa caaaaatccc aatattttgt tttatgtctc
     2581 aaatgcaata tagcgtagat tttaaaaatg tcaagcatta tatcgatttc gatgaaatat
     2641 aatctatcac attactcctt tgtaaatgta attttcgcta attttactac caatactatt
     2701 atcggcatac tacgttatac tataatatga gtgattgctc actcatatta tagaactata
     2761 aaattgtcaa tacattttca tattctttaa caaaattact ggtcaagcct acaatataca
     2821 attcaacgta tgcttttaga ttactaacac gaaggttatt tatatttcta atttgaacca
     2881 ccaatcggtg ctaaaaacaa caaatcggta attgggacta ttggtacccg tttggtatgg
     2941 acttcatata aacttaaaga agaccatctt caaatgggat taaaaaaagg tgatgtatgg
     3001 aatgactatc tgtatggcgt ttttgtaagt gttgtctata tgatgtaaaa aatttatgta
     3061 tactatagtg aaaatttccg gtaactatcg ccaaaaaatc taaaaaaatt tattttttgt
     3121 tttacatttt catgtaatat ggagtaacat acatttgatt atgtaataat acgttggttt
     3181 agacattatg aatgtcataa ttttttttca actgaatctg gaggatactt atgtttcttg
     3241 gtaaaaaagc tttgagtata gtattggttc tgttattgtc atttgttttt tcatgctcat
     3301 cccaggacaa agcaaagact ttgcgaaata ctcctcgtga actcagaaaa ggacttttaa
     3361 tacttaattt ctataattca actcccaaga gtcgtgcttc tgagtttgaa ccatgggaat
     3421 atggacttgc atcaatgctt atgaccgata tggagtcaat tggcgtgttc aacattatat
     3481 caaaagaagc gttaaaagat gttattgctg aacaggaatt tcaacagact ggcatgattg
     3541 atcctaaaac tctggttaaa ttaggcaatt tagttggtgc acagtatatc ctcaagggct
     3601 ctttcatgga aatgaatggc atgttacgca ttgaaacgca ggttttctct gtagaaaaag
     3661 gggtacaact ccaaactgca gttgtgaatg gtaaaacaga tactttcttt acacttgaaa
     3721 aagaacttgt agataagata acccagattg ttgatgttgt actgactgaa tctgaaaaga
     3781 atgtgatact ggccaagatt gagacaaaat cagttgaagc ttcattaaaa aattacaaag
     3841 gtgagttaac cgttgaagaa gctaaaaagt tagcacagga gggtaaaaaa gaagaagcta
     3901 aaaaactcat agaacaggca aaaaaggatt ttaaagaagc tgttgccata gacccaaatt
     3961 atgaaaaagc taagaagaac ctttcaaaga tttcaatggc aataccagta acgctgtagt
     4021 attgaacatt tcaaagttta aatattattt caagaagata tacagtatca aatataaagg
     4081 ggcgtaggaa tacgcctctt tgattttata tttacttttt ttacttcacg atataaaaac
     4141 tatacccaat cattccgcca aatgtccaga agaagacatc acgtattctg tcttctatgt
     4201 gtaattttcc tccacgcaca aaatattgca aaaattccag agctaatcct gcaaaaagcc
     4261 cggcaaaagc agaatatgga aaaccatcgg tgatatatgc aacaccaaat gtgcccacca
     4321 acccgtacaa tgtatgcaca tgctgattca acacttcttt tcgtggccag aaagcctttg
     4381 aaaatatatt aacctttaac atcagcaata acacaataaa cagtaacagt attatgcaaa
     4441 gaattccaag aacccccaca gtgcacctct acagatgtaa taatagcaac ggtatcccca
     4501 accatgactt ttgtcaagaa atgctcaacg acaaaagatt gtcatgtttt tttcaactgg
     4561 gaatccacta ttcatctttt agcttacaat aaaaaatttt ttattgattt gactttgtac
     4621 acgcgtatgt gttacaagcg tttttactta ttg
//
LOCUS       DAORYL010000136         4525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_12930_length_4525_cov_5.143177, whole genome shotgun sequence.
ACCESSION   DAORYL010000136 DAORYL010000000
VERSION     DAORYL010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12930_length_4525_cov_5.143177"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="PLV81_14690"
     CDS             <1..1254
                     /locus_tag="PLV81_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015419869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM11385.1"
                     /translation="STLPSYIPRSITIPTNSGKIITLVGPRRSGKTYMLYQIIDDIIK
                     AGNSKENVLYINFEDERLDLQISEMDIILQAYRELYPSINFQKTYFFFDEIQNINGWE
                     KFIRRIYDSISKNIYITGSNSKLLGEEIATSLRGRTLKYEVLPLSFEEFLIFKKTKIE
                     IPQDFYNSSVQAKLINLFHEYMTWGSFPEIVFQSNELKLKILQEYYDVMIYRDLIERY
                     KITDTFILKYLIKRLAETITKPLSINKIYNELKSQGIKIGKNTLYEYLEYLENAFLIR
                     KILKYGRSVVRSELSDKKAYIVDNGILRAIKIYEKEDFGILLENLMFRELHRKTGKIS
                     FYKDQKECDFVVNEKIPVQVCFDLSHDEVKKREIAGIMACCKQLHAQKGYIITFDDED
                     SLFTNGCTYEIIPAYKWLLEQDRYI"
     gene            complement(1251..2102)
                     /locus_tag="PLV81_14695"
     CDS             complement(1251..2102)
                     /locus_tag="PLV81_14695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOM11386.1"
                     /translation="MNNLVKAYNDIREIVLSDSFLRNIPSVDRTLAYDIILQSLHEAQ
                     WAIRFLSQFDCNGKRVLEIGAGAGIVSACLHLCGAQVTMIEPSSGAFSYHEKICASLH
                     KHLGIQIPCITKPVEEIEPGLPQYDFIFSINVLEHILNLDKAFSVMNYLLKYDGVMFH
                     ICPNYTIPYEPHVGVVLVPFFPRATFTLSKRLRTHPVSEQLTFITVSSIKKLAKKNNL
                     TVKFYKDMMYQSFMRLTTDSEFRKRQSGLPATVFKLLQSLGIIHFTKYVPVCLQTPVA
                     FEMRKKF"
     gene            complement(2099..2938)
                     /locus_tag="PLV81_14700"
     CDS             complement(2099..2938)
                     /locus_tag="PLV81_14700"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOM11387.1"
                     /translation="MIERIEQHLVCIQCKVKMQRNGNAFECRACNQKYALHNNALFIV
                     SGSTDKVINPLDKIKLLLKPFSRLYALLIKIVSPVYVGKTLSSFVKHNIFEGRLYLNL
                     GSGNMRLHQDIINVDFIHYPNVDLVADISSIPLVNECCDGVYIIGVLEHVENPCTVID
                     EVHRLLKPGGFVYSFFPFMQGYHASPHDYFRTTHEGVKTLFKDFNDITIVLAGGPTSG
                     FLWVLQEWLAMILSLGIRPLYYFWLIVLMCMTFPIKFIDIILLHHPMAANITSGFTIV
                     AKK"
     gene            complement(2949..3317)
                     /locus_tag="PLV81_14705"
     CDS             complement(2949..3317)
                     /locus_tag="PLV81_14705"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11388.1"
                     /translation="MNQKIMPFICIAMTVLCTVYGQLITKWRVNALGQTSSPLLSYYK
                     ILFFDPLFLSGLVAVIPAGFCWIYAIRHIQLSVAYPFIGLTFALVLLGSAVLLREPVT
                     IAKIAGTLLIIAGISIIAIK"
     gene            complement(3435..3734)
                     /locus_tag="PLV81_14710"
     CDS             complement(3435..3734)
                     /locus_tag="PLV81_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11389.1"
                     /translation="MNNQLATRQDVTLSELKLTKEIEHIRADLRETELKLTKEIEQVR
                     AEMKETELRLIKEIQQVRVEIQQTKVIILKWVIGLLITQTVTIVGVIIGLFQVLK"
     gene            complement(4209..>4525)
                     /locus_tag="PLV81_14715"
     CDS             complement(4209..>4525)
                     /locus_tag="PLV81_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11390.1"
                     /translation="RLIRLETKLETALQQIDKRFEQIDKRFEDLFTFLWILTGIFTTL
                     TLSSIGFAYWDRRTAIRKAKEETIATIEREGSLVQLINALRELAKKNKEVASVLRMFN
                     LL"
BASE COUNT         1462 a          836 c          790 g         1437 t
ORIGIN      
        1 tcaacactac cttcatatat tccacgtagt attactattc ccactaacag cggtaagatt
       61 attacgcttg ttggaccaag gcgaagtgga aaaacataca tgctctatca gattattgat
      121 gatattataa aagcgggaaa tagtaaagag aatgtactat atataaattt tgaagatgaa
      181 cgcctggatc tacaaatttc cgagatggat ataatacttc aggcttacag agaattatat
      241 ccatcaatca attttcagaa aacatatttt ttctttgatg aaattcaaaa tattaatggg
      301 tgggagaagt ttataagaag gatatatgat tcaatatcaa aaaatatata tatcaccggc
      361 tcaaattcaa aattgttagg agaagagatt gctacatcat taaggggtag aacgcttaaa
      421 tatgaagttc ttccattatc atttgaagag tttttaattt ttaaaaaaac aaaaatagaa
      481 attccacaag acttttacaa ttcttctgta caagccaaac ttattaattt gtttcatgaa
      541 tatatgacat ggggaagctt tcctgaaatt gtttttcaaa gcaatgagct aaagcttaaa
      601 attcttcaag aatactatga tgtaatgata tatagagatt taattgaaag atataaaata
      661 accgatacgt tcatattgaa atatttaatc aagcgcctgg ccgaaacaat aaccaaaccg
      721 ctttccatca ataaaattta caatgaacta aaatctcaag ggattaaaat tggtaaaaat
      781 acactctatg aatatctaga atatctggaa aatgcgtttt taattagaaa aattttaaaa
      841 tatggacgtt cagttgttcg tagtgagctt tctgacaaaa aagcatatat tgttgataat
      901 ggaattttgc gcgcaataaa aatatatgaa aaagaagatt ttggaattct ccttgaaaat
      961 cttatgttca gggagttgca cagaaaaaca ggaaaaattt ctttttataa agatcaaaaa
     1021 gagtgcgatt ttgtggtaaa tgaaaaaatt cctgttcagg tttgttttga tttaagccac
     1081 gatgaagtaa aaaaacgtga aattgcaggg ataatggcct gctgcaaaca attgcatgca
     1141 cagaaaggat atatcataac atttgatgac gaggacagtt tatttactaa tggatgtacg
     1201 tatgaaatta tccctgcata caaatggctg ctggagcaag acaggtatat ttaaaatttc
     1261 ttcctcattt caaatgctac gggcgtttgc aggcatacag gcacgtattt agtaaagtga
     1321 attatcccta atgattgtaa taacttgaac acggttgcag gtaaaccgct ctggcgtttt
     1381 ctaaactcgc tgtctgttgt aagacgcatg aacgactgat acatcatgtc tttataaaat
     1441 ttcacagtaa ggttattttt ctttgccaat ttttttattg agcttacggt aataaacgta
     1501 agctgctcgg aaacgggatg tgtgcgcaag cgctttgaaa gggtaaacgt agcgcgcggg
     1561 aaaaacggca caagcacaac ccccacatgc ggttcatacg gtatggtgta attgggacat
     1621 atatgaaaca taactccatc atattttaag agatagttca taacactgaa ggctttgtct
     1681 aagttcaaaa tatgctccaa cacatttatg gaaaaaatga aatcatactg cggtaaaccc
     1741 ggctctattt cttcaacagg tttggtgata cacggtattt gaatgccaag atgtttatga
     1801 agtgacgcac atatcttttc atgatagctg aatgcaccac tggaaggctc tatcatggtt
     1861 acctgtgcgc cacacagatg caaacaggcg ctcacgatcc ctgctcccgc accaatttca
     1921 agcacccttt taccattgca atcaaactgc gaaagaaagc gtattgccca ctgtgcttcg
     1981 tgcagagact ggagaatgat atcataagct aaagttctat caacagaagg aatatttctt
     2041 aaaaaactat cggatagtac aatttccctt atatcattat atgcttttac cagattattc
     2101 atttttttgc aactatggta aatcctgagg tgatgtttgc tgccatggga tgatgtaaaa
     2161 gaattatatc gataaattta attggaaacg tcatacacat cagcacaata agccagaaat
     2221 aatacaatgg cctgattccc agcgataata tcatggcaag ccattcctgc aatacccata
     2281 aaaaccctga tgttggaccg cctgcaagca caatggttat atcgttaaaa tctttaaaaa
     2341 gagtttttac cccttcatgt gttgttctga aataatcgtg cggcgacgcg tgatatccct
     2401 gcataaatgg aaaaaacgaa tacacaaaac cacctggctt aagcaggcga tgaacttcat
     2461 caataaccgt acaaggattt tccacatgtt caagaacgcc aataatatat acgccatcgc
     2521 agcattcatt taccagagga attgaggaaa tatcggccac cagatcaaca ttgggatagt
     2581 ggataaaatc aacattgatt atgtcctggt gcaggcgcat gttgccactc ccaagattta
     2641 aatagagcct cccttcaaaa atattatgct tcacaaatga tgatagtgtt ttcccaacat
     2701 acacggggct tacgattttt attaaaagcg cgtacagtct gctgaagggt ttaagcaata
     2761 attttatttt atcaagaggg tttatcactt tatcggtgct gcctgaaact atgaataaag
     2821 cgttattgtg aagcgcatat ttttgattgc acgcgcggca ttcaaaagca ttgccattgc
     2881 gctgcatttt gaccttgcat tgtatgcata cgagatgttg ttcaattctt tcaatcataa
     2941 aaatttttct attttatagc aataatactt attcctgcaa taatgagcaa tgttccagct
     3001 atctttgcta ttgtaacggg ctcgcgcagc aacacagctg atcctaaaag caccagcgca
     3061 aaagtaagac caataaaagg ataggcaaca cttaactgaa tatgacgtat ggcgtatatc
     3121 cagcaaaaac ctgcgggtat aaccgccaca agtcctgata aaaataatgg atcaaaaaaa
     3181 agaattttgt aatatgataa aagcggcgat gaggtttggc caagggcatt gacacgccat
     3241 ttagttataa gctgcccata cacggtacac agtaccgtca tggcgatgca tataaagggc
     3301 attatttttt gattcatgat tttcctaaaa ttattttatt ttttatatta ctgctgtaaa
     3361 gaatatatta tattattgtt accctatatt tactgatatg cataattgcc ttgtttactt
     3421 atacatcaaa acttctactt caatacctga aataacccta taataacacc tactattgtt
     3481 actgtctgtg ttatcaacaa tcctattacc catttcaata taataacttt tgtttgctgg
     3541 atctctaccc gtacctgctg tatttctttt ataagcctta attcagtttc tttcatctct
     3601 gcccgtacct gttctatctc ctttgtaagc tttaattcgg tttcccgtaa gtctgcccgt
     3661 atatgctcta tctcttttgt cagtttcaat tctgataatg taacatcctg gcgcgttgca
     3721 agctgattat tcataattgc ctgctccacc atctctataa actccgttaa aacttttgcc
     3781 tttacctcat catctttgaa tgcttcatat acccgtattg taaatgccag tgtattgtat
     3841 ctttcctttt aatttttgta tttactctta tctcaacata tatattgtcc accaatattt
     3901 caagaagttt ttattaaata tattatatat ggaaaaattt ttggatggct tcgttggttt
     3961 ggctcaaaat gacaaagaaa ttattaaata agatttctcc tcactgcgtt cgtcgaaatg
     4021 acaatgggaa aacgttcgtc aaaatgacgg cttttttatg tcatcctgag cgaagcgaag
     4081 gatccccctt aggctttcaa gacattcctt gcctttggct cagaatgacc gtgttacctt
     4141 gctacaattc atcattaaac ttgtcgtggt atggcaattg agcttgtcaa gatgcgtgcg
     4201 tactgtaatt aaagaagatt gaacatccgt aaaacactgg ccacttcttt attctttttt
     4261 gccagttctc ttagggcatt aatcaattgc acaaggcttc cttcccgctc aattgttgca
     4321 attgtttctt cttttgcttt acgtatggca gtgcgcctgt cccaatacgc aaatcctatt
     4381 gatgaaagag taagtgtggt aaaaatacct gtaagaatcc atagaaatgt gaataagtcc
     4441 tcaaaacgtt tgtctatttg ttcaaaacgc ttgtctattt gctgaagcgc cgtctcaagt
     4501 ttggtttcta accgtatgag ccgct
//
LOCUS       DAORYL010000137         4481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_13078_length_4481_cov_10.436286, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000137 DAORYL010000000
VERSION     DAORYL010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13078_length_4481_cov_10.436286"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="PLV81_14720"
     CDS             <1..1118
                     /locus_tag="PLV81_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11391.1"
                     /translation="VGWGNINSTETSALRYLFHLNRADNIEENFIKGIDLSSEELLED
                     LQKIEQDSGGVIAVKEISDITLKPFDAVVTVYPEPKGVRVIERSVPQGWQLTSYTSLS
                     YRREDEKSDEIQIPVVSKEEIKEHSVFTFPAGSISGLCIHKIFETIDFIADSTSILHI
                     ASDVLKQYNIDTVWADVVTKMVVNVLKVQLQKEKDLVLSKIHDNKRRHEIEFYYPVEQ
                     LDSRHLVQLFNEHSLFTMDYASALQQLSFEDISGFMHGYIDMVFEYENAYYIVDWKTN
                     LLGTSLEDYHYTKLRSAIVSHLYFLQYSIYTVALHRYLQLLLGKGYSYDKHFGGVFYI
                     FVRGVDANRGNEYGIFYDKPDVTFVHKFDNLLGALKV"
     gene            1115..>2089
                     /locus_tag="PLV81_14725"
     CDS             1115..>2089
                     /locus_tag="PLV81_14725"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOM11392.1"
                     /translation="MKLIDKTKTIHHHFANFISSKYHCYDEVLNALLFCVSYMAANGH
                     SCLSLGTIANKSLRNIIYDESIEIDDIVFPDEKSLEQYVLDCDAFAKTEHHIKPLVYR
                     DGNCYLYRYFNYENTILQFFILSQGEIKTIYEDCNKAIEELQSKGIHFSNEQVLAQLT
                     ALKHRFCVVSGGPGTGKTTVVAAIVYQLLRNNPDCSIALCAPTGKAASRLMQAIVHFK
                     ETYVANSHMNFPAEAFTIHRLLGYQKGELEFYYNEQNHLPYDVVIVDECSMVDIPLMA
                     KLTKALKSTARLILVGDKDQLSSVEAGAFFGDVCWGTTEFSYTDSGKKL"
     assembly_gap    2090..2189
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2190..2666
                     /locus_tag="PLV81_14730"
     CDS             <2190..2666
                     /locus_tag="PLV81_14730"
                     /inference="COORDINATES: protein motif:HMM:NF024928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HOM11393.1"
                     /translation="GPWGVETFNALIEKQLRPAISGEWYNGRIIMITANDYNLEVFNG
                     DVGIHKSSDDAVYFVSADGLRKINTSLLTNYVPAYSITVHKSQGSEFNHVVLILPHID
                     IGNNEHWKMLLTRELLYTAVTRARNKLTIIGNKKILQYMMKNPTIRSSGLRQRLWQ"
     gene            2784..3740
                     /locus_tag="PLV81_14735"
     CDS             2784..3740
                     /locus_tag="PLV81_14735"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOM11394.1"
                     /translation="MNILILGGGAVGLGLARSLITAGNDVTIIDVEPTVSALKEKGIM
                     QTGVLGEFTIQPGIFKVYDSLAKIGNVKIDYICVCIKSYLSETIAQVLKQYEHIIGTC
                     PIVLFQNGWGNAEQFIKYFEKDTIFNARVMTGYIKPALHRVDITVHADAIHIGSLYGG
                     DITQLLSLASELTKGGMPAEVTEHVSKDIWAKMLYNCALNPLGAIFNVPYGELGKSEY
                     TKAIMNDIFHEIFETMHKAGYSTHYRSAEEYIDVFYSRLLPSTGEHRSSMLQDIQAKR
                     RTEIDAMNGAVVALAKQHGVDTPVNEVITGLIKFIEGGYHGK"
     gene            3730..>4481
                     /locus_tag="PLV81_14740"
     CDS             3730..>4481
                     /locus_tag="PLV81_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HOM11395.1"
                     /translation="MANSLVRSGNPVLRSDPFTKEIGEPATDVMTIEGAVNKTIILLL
                     IVVGFATLSWYHIENLLFNNYLHMFLIVLSMLGFIVALLTIFMKKLSPWTAPVYSAIQ
                     GLFLGIVSMLFEKSFPGIVLQAVGLTFGILFSLLLAYKSGLIKATENFKLGVAAATGG
                     IFVVYLCNFILRFFGIDIPFIHEGGILGILFSLFVVVIAALNLVIDFDFIEKGAEYGA
                     PKYMEWYAAFGLMVTLIWLYLEILRLLAKTRR"
BASE COUNT         1368 a          664 c          922 g         1427 t
ORIGIN      
        1 atgttggatg gggaaatata aatagtacgg aaacatcagc tttgcggtac ctttttcatc
       61 tcaatagagc cgacaacata gaagaaaatt ttataaaagg tattgacctt tcttctgaag
      121 aattgttgga agatttacaa aaaatagaac aggattcagg gggcgtcatt gctgttaaag
      181 aaatatcgga tatcacacta aagcctttcg atgctgttgt taccgtatat cctgaaccaa
      241 agggggtgcg tgtaatagaa cgatcggttc cccagggatg gcagcttaca agctatacat
      301 cgttatcgta caggcgcgag gatgaaaaaa gtgatgaaat acagattcct gttgttagta
      361 aagaagaaat aaaagagcac tctgttttta cgtttcctgc aggaagtatt agtggtttat
      421 gcattcataa gatttttgag acaattgatt ttattgccga tagtacaagt atcttgcata
      481 ttgccagtga tgttctgaaa caatacaaca ttgataccgt gtgggctgat gtcgtaacaa
      541 agatggttgt caatgtgcta aaagtacaat tgcaaaaaga gaaagatctt gtgctatcta
      601 aaatccatga caataagcgg cgtcatgaaa ttgagtttta ttatccagta gaacaacttg
      661 atagcaggca tttagttcag ctttttaatg aacacagcct ttttaccatg gattatgcat
      721 cagcactgca acaattatca tttgaagata taagtggctt tatgcatggc tatattgata
      781 tggtgtttga atatgaaaat gcctactata ttgtagactg gaaaacaaat ctgcttggca
      841 cttcattaga agactatcat tacactaagc taagaagtgc aattgtttcg catctatatt
      901 tcttgcagta ttctatctat actgttgccc ttcacagata tttacaatta ttgttaggca
      961 agggatatag ttatgacaaa cattttggcg gtgtgttcta tatatttgta cggggagttg
     1021 atgctaaccg gggaaatgaa tatggaatat tttatgataa accagacgtt acatttgtac
     1081 acaagtttga taacctgtta ggtgctctga aggtatgaaa ttaattgata aaaccaaaac
     1141 cattcatcat cactttgcca actttatttc aagtaagtat cattgttatg atgaagtatt
     1201 aaatgcattg ctattttgtg taagctacat ggcagctaac ggacatagct gcctttcatt
     1261 gggaactatc gccaataaat cattgcgcaa tattatctat gatgaaagta ttgaaattga
     1321 cgatatagta ttccctgacg aaaaatccct ggaacaatat gtactggatt gtgacgcttt
     1381 tgcaaaaact gaacatcaca taaagccact tgtgtaccgg gatggaaact gttacctgta
     1441 tcgttacttt aattatgaaa atacaatctt gcaatttttt atactatcac agggtgaaat
     1501 caaaactatt tatgaagatt gcaataaagc cattgaagaa ttgcaatcaa aagggattca
     1561 cttttcaaat gagcaggttc ttgcacagtt gactgcgttg aaacacagat tttgtgtagt
     1621 ttccggtggg ccaggcacag gtaaaaccac agtagttgct gcaatagtat atcaattgtt
     1681 acgcaataat cctgactgtt ctatagcatt atgtgcacca acggggaaag cagcatcgcg
     1741 tttaatgcag gcgatagttc attttaaaga gacgtatgtg gccaatagtc acatgaattt
     1801 cccagctgaa gcttttacca ttcaccgact ccttggctat cagaaaggcg agcttgaatt
     1861 ttattataat gaacaaaatc atttgccata tgatgtggtt attgttgatg agtgttctat
     1921 ggttgatatt ccgcttatgg caaaattgac taaggcgtta aaaagcactg ctcgtttaat
     1981 tttagttggt gataaggatc agctatcatc ggttgaggcg ggtgcttttt ttggtgatgt
     2041 atgctggggt actacggaat tttcatatac tgatagtgga aagaaattan nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gaccgtgggg agtagaaaca tttaatgcgt
     2221 tgattgaaaa acaattaaga cctgcaatca gtggtgagtg gtataatggt agaattatca
     2281 tgattactgc caatgactat aaccttgagg tgttcaatgg tgatgtgggt attcataaga
     2341 gcagtgatga tgcagtgtat tttgtatctg cagatggttt aagaaaaatc aatacatcac
     2401 ttttaacaaa ctatgttcct gcgtacagta taacggtaca taaaagtcag gggtcagaat
     2461 ttaatcatgt agtgctgatt ttaccgcata ttgatattgg caataatgag cactggaaga
     2521 tgttattaac gcgtgaactt ctttataccg cagttactcg tgcacgcaat aagcttacta
     2581 ttattggaaa taaaaaaatt ctgcaatata tgatgaaaaa tcctaccatt cgctcttctg
     2641 gtttgcggca gcgattgtgg cagtaactta taaaaaaaga ccttttatgt aacttcctgc
     2701 tattttgatt ttttgcttga gcttacaaac gtatatggca ttctggaatg gaaatgctat
     2761 ctttactata aaaggtattg cccatgaata tattaatact tggtggtggt gctgttggat
     2821 tgggtttagc gcgaagcctt attactgcag gcaacgatgt aactattatt gatgttgaac
     2881 caacggtcag tgcactgaaa gaaaaaggta ttatgcaaac aggtgtgttg ggggaattta
     2941 ctatacagcc tggtatattt aaagtctatg attcattggc aaaaataggc aatgtcaaaa
     3001 ttgattatat ctgtgtatgt attaaatcat acttatcaga aactatcgca caggtattaa
     3061 aacaatatga acatataatt ggtacctgtc ccatagtatt gtttcagaat ggatggggca
     3121 atgctgaaca atttataaaa tactttgaaa aagatacaat cttcaatgcg cgtgttatga
     3181 caggatacat taagccagcg cttcaccgtg ttgatataac ggttcatgct gatgcaatcc
     3241 atattggttc attgtatggt ggtgatatta cacaattact atcgcttgcc agtgagttaa
     3301 caaaaggtgg catgcctgct gaagtgaccg aacatgtgag taaagatata tgggcaaaga
     3361 tgctgtacaa ctgcgcgtta aatccattag gtgctatctt caatgtaccg tatggagagt
     3421 taggtaagtc tgaatataca aaagctatca tgaatgatat atttcatgag atatttgaaa
     3481 ccatgcataa ggcaggttac agcacgcatt atagaagtgc agaagaatat attgatgtat
     3541 tttattctcg attgcttcca tcaacggggg agcatcgttc atcaatgctg caggatatac
     3601 aggcaaagcg ccgtactgag atagatgcaa tgaacggagc tgttgttgca ttagctaaac
     3661 agcatggtgt tgatacacct gttaatgaag tgataacagg attaattaaa tttattgagg
     3721 gagggtatca tggcaaatag tcttgttaga agcggaaatc ctgtgctcag aagtgatcca
     3781 tttacaaaag aaattgggga acctgcaact gatgtaatga ccattgaagg cgctgtgaat
     3841 aagactatca ttttattact gatagttgtt ggttttgcaa cgttaagctg gtatcatata
     3901 gaaaacttgc ttttcaataa ttatctccat atgtttctga tagttttgtc aatgttaggt
     3961 tttatagtag cacttcttac tatttttatg aaaaaactat caccatggac agcacctgtt
     4021 tattctgcaa tacagggctt atttcttgga atagtatcaa tgctatttga aaaaagtttt
     4081 cctggtatag tgttgcaggc agttggactt acctttggaa tacttttttc gcttcttctg
     4141 gcttataaat caggtttaat taaggctact gaaaatttta aacttggtgt ggctgctgca
     4201 acaggtggaa tatttgtggt ttatttgtgt aattttattt tacgattttt tggtatagat
     4261 attcctttta ttcacgaagg tggtatatta ggcattcttt tttcactgtt tgtagtagtt
     4321 atagcagctt taaatcttgt gatagacttt gactttattg agaaaggtgc tgaatatggt
     4381 gctccaaaat atatggagtg gtatgctgct tttggtttaa tggttacttt gatatggtta
     4441 tatcttgaga tattacgatt gcttgcaaaa acaagaagga g
//
LOCUS       DAORYL010000138         4459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_13156_length_4459_cov_12.587875, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000138 DAORYL010000000
VERSION     DAORYL010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13156_length_4459_cov_12.587875"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1533
                     /locus_tag="PLV81_14745"
     CDS             <1..1533
                     /locus_tag="PLV81_14745"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOM11396.1"
                     /translation="RRDLIKKSIAAGVAVGAGTLIGTCTYKLLKTPDITDVYGNYPPE
                     EKLKKLTVVNPRAPRPNVIIIYCDDLGYGDIGCYGNSVIHTPNIDSLAREGNRFTNFY
                     ACAAVCAPSRAGLLTGRYPFRTGIIGNPFPKQEPLGRKLARNFGMMLRGLGSMDLRDD
                     VVARGLAFEEVTIAEALKLAGYKTGMVGKWHLGDYSTQPEFNPLRHGFDFYYGVPHSN
                     DMRPCPVYRNETKVMDNIHGEDQSFLTGAYTKEALQFIESCGNNPFFLYFAHTFPHQP
                     LWASEKFNKKSLAGKFGDAVEEIDWSVGEICKLLKERGLDDKTLIIFTSDNGPWYEGS
                     TANLRGRKGNSNEGGFKVPFIVKWPGVVPQGKITHEPAMNIDLFPTLLGLAGVGLPED
                     RIIDGKDILGLFKGNDKPVHEDLYFYHYDLLVGIRSGKWKYYSRIDRYVWPIPLDSTD
                     LANALGKDQLGNRWPLLYNLDKDPGEAYNVIDTYPDIARKLRQKLENWEKETMKNPRG
                     FK"
     gene            complement(1534..2163)
                     /locus_tag="PLV81_14750"
     CDS             complement(1534..2163)
                     /locus_tag="PLV81_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11397.1"
                     /translation="MNLNHPEATQQALSILRSGNPFQWYVITMLAFVVYIYFNEIQNK
                     NWKGIAAGLSLYMVHWFVEIINALIQHFTGHALWTVPTGTAFLILIGVGVELSLMFSV
                     SGLIFSKILPEDPKAKILGINNRLFIAIANAAFYSIFEIFLVKTPCFVWVYPWWGALP
                     VFITVYIPFWVVSLYCYDWQPKVQKAVIGSLFAINAGMLVVFAGILKWI"
     gene            2516..3172
                     /locus_tag="PLV81_14755"
     CDS             2516..3172
                     /locus_tag="PLV81_14755"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOM11398.1"
                     /translation="MVKNIFINGLIIMIAICSLHCKKDESIKPANAIIVLAQGVVSIN
                     DIQAHTGVSLHYGDVIKTGDDGICRIQIGDRNVLQIGKSSELVFNISDTGNELLLNQG
                     WLGGVTRKIFTSGGKYLVKTPTVVASIRGTSFCVVATSVEKSYFCVCNGSINLMGSSM
                     EKGEDVTAAHHKGLWFQKDKNGNVVVKEAGLLFHDDKGIEELAASINQTIDWTKPDTY
                     "
     gene            complement(3384..4367)
                     /locus_tag="PLV81_14760"
     CDS             complement(3384..4367)
                     /locus_tag="PLV81_14760"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOM11399.1"
                     /translation="MADSIYRELSKRLMFENSQLLQQIWQRVANEIEAEIICYLPANA
                     AQVAEKFNLSVDEATRILEELFKRGAAFKKSKPEGAEYRPPKHIVQFHDATLLWRDAP
                     ADFKKLWVEFMNTEYPPLLEVVTKANIPSFMRVIPINATIQPKSEVLVYEDAYRIIEQ
                     AKELAVVDCVCRLSHGNCSKPLNVCIQVNNGATYTLERGTGRAITKEEAFEILKFSEE
                     AGLVHMTENKGFGNAICNCCSCCCEMLRFAGNRATHGVVAKSRFAAIVDTNNCNGCSL
                     CTSVCPVHAIEVNEIAAVDAEMCIGCGLCATHCPSGVITLHETRPKEHIPV"
BASE COUNT         1356 a          808 c          946 g         1349 t
ORIGIN      
        1 cgccgtgatt tgataaaaaa atctattgct gctggcgttg ctgttggtgc tggcacattg
       61 attggcacct gtacgtataa gttgctgaaa acacctgata ttactgatgt atatggcaat
      121 tatccaccag aagaaaaatt aaaaaaattg actgttgtaa atcccagggc acctcgaccc
      181 aatgttatta tcatttattg tgatgatctg ggatatggtg atattggctg ttatggcaat
      241 tctgtcatac acactcccaa tattgattca ctggcacgag agggtaacag gtttaccaac
      301 ttctacgcct gcgctgcggt atgtgcacct tcacgtgcgg ggttattaac agggcgctat
      361 cctttcagaa ccggtatcat tggcaatcca ttcccaaaac aggaaccgtt aggtcgcaaa
      421 ttagcgcgta attttggtat gatgcttcga ggtttggggt caatggattt gcgtgatgat
      481 gttgttgcca ggggattggc ttttgaagaa gtaactatcg ccgaagcttt aaaacttgcc
      541 ggatataaaa ccggtatggt tggcaaatgg catctgggcg actattcaac acagcctgaa
      601 tttaatccac tgcgccatgg ttttgatttt tactatggtg taccacacag caacgatatg
      661 cgaccatgcc cggtatatag aaatgaaacc aaagtaatgg ataatattca tggagaagac
      721 cagtcatttt taaccggtgc atatacaaaa gaagccttgc agttcattga gtcgtgtggc
      781 aacaatccat tctttttata ttttgcccat acatttcctc atcagccact atgggcttca
      841 gaaaaattta ataaaaaatc attggcaggc aaatttgggg atgcggttga ggagattgac
      901 tggagtgtgg gggaaatatg taagctcttg aaagaaagag ggctggatga caaaaccttg
      961 ataatcttta ccagtgacaa cggaccgtgg tatgaaggga gtacagctaa tttgcggggc
     1021 agaaaaggaa atagtaatga aggtggattt aaggtgccgt ttattgtaaa atggcctgga
     1081 gtggtaccac agggcaaaat tactcatgag cctgcaatga atattgatct tttcccaacg
     1141 ttacttggac ttgcaggtgt tggattgcct gaagatcgca tcattgatgg aaaggatata
     1201 ctggggcttt tcaaaggtaa cgataagcca gtgcatgaag atttatattt ttatcactat
     1261 gacctgcttg ttggcattcg ttccgggaaa tggaaatact attcaaggat tgatcggtat
     1321 gtgtggccta tcccacttga ttcaactgat cttgctaatg cactgggcaa agaccagtta
     1381 gggaatcgat ggccattgct gtataatctg gataaggatc ccggggaggc atataatgta
     1441 atagatacat atcctgatat tgcccgtaaa ctacgccaga agcttgaaaa ctgggaaaaa
     1501 gaaactatga aaaatcccag agggtttaag taattaaatc cattttaaga ttccagcaaa
     1561 aacaaccagc attcccgcgt taatggcaaa gaggctacca attactgctt tttgtacttt
     1621 tggctgccag tcatagcagt acagcgatac aacccagaat ggtatataca cggtgataaa
     1681 taccggcagt gcaccccacc atggataaac ccatacaaag cacggtgttt tcaccagaaa
     1741 tatttcaaat atagaataaa aagcagcatt ggcgatagct ataaataaac gattgttgat
     1801 gccaagaatc tttgccttgg ggtcttctgg taaaatcttg ctgaagatta aaccggatac
     1861 tgaaaacatt aagcttaatt ccacgcccac accaatcagt ataagaaatg cagtccccgt
     1921 gggtaccgtc cacaaagcat gtccggtaaa atgctgaatg agtgcattaa ttatttccac
     1981 aaaccagtga accatgtaaa gcgaaagccc tgctgcaatg cctttccagt ttttattctg
     2041 gatttcatta aagtagatat agacaacaaa agctaacatg gtaatgacat accactgaaa
     2101 agggttgcca ctgcgtaaaa tactgagtgc ctgctgtgtt gcttcaggat gattgagatt
     2161 cataattcct ccccgtgaga taagatttat gatttttgaa agtggtgttt atacttgcat
     2221 gccaatcttt gggaatatca tgcaccatag gttttgtctt cctcaccatg tgaattgttt
     2281 ataatgaaat acgtcacgta ttttcatgtt ttcaatggtt actatgcagc aatagtctgg
     2341 ttaccatact attacatctc attattataa gaagtgtata gtgtttatca tgaattgtta
     2401 agtattgaat aaaaacatta ttacaaatgg aaggggaatg caagaaataa tttcattgac
     2461 tttccttttt ttatatttat gatatgttta atgaatgata gtaatggagg atcgtatggt
     2521 aaaaaacata tttattaatg gtcttataat aatgatagca atttgcagtt tgcattgtaa
     2581 aaaggatgaa tcaataaaac cagcaaatgc tatcattgtt cttgcccagg gtgttgtcag
     2641 cataaatgat attcaggcac atacaggggt atcattacac tatggtgatg ttattaagac
     2701 tggtgatgat ggaatatgca gaatacagat tggtgacagg aatgttttgc aaattggtaa
     2761 gtcttctgaa ttagtattta atatatctga tacagggaat gaactgctct tgaaccaggg
     2821 atggttggga ggagttaccc gaaagatttt taccagtggt ggtaaatatt tagttaaaac
     2881 accaacggtt gttgcaagta tacgaggtac ttcattttgt gttgttgcta cttctgttga
     2941 aaaatcatat ttctgtgtat gtaatggaag tattaattta atgggaagca gcatggaaaa
     3001 aggagaagat gtaacagcag cccatcacaa aggattatgg ttccagaaag acaagaacgg
     3061 gaatgttgta gttaaagagg cgggtttgct gtttcatgat gacaaaggca tagaagagct
     3121 tgctgcaagc atcaaccaaa ctatagattg gacaaaacct gatacttatt aagcatgtat
     3181 gttagtcaaa atcatgactt attccagata taatggtctt gttatataat tttatcatac
     3241 atgtttgtaa agtaataact gatcaacaat tttaacataa tatgtaaaat aggtatacca
     3301 atgtgaattg cggcggaaaa ggggggattt tcatttatat cataaaaatt gagttagcga
     3361 caattactat ataatctaaa agattatacc ggaatgtgtt cttttggtcg tgtttcatga
     3421 agcgttataa cgcctgatgg gcaatgtgta gcacacaatc cgcaccctat acacatctca
     3481 gcatcaactg ctgctatctc attcacctct attgcatgta cgggacacac agaagtgcat
     3541 agtgagcagc cattgcagtt gtttgtatca acaatggcag caaatcggct ttttgcaaca
     3601 accccgtgag ttgccctgtt accggcaaaa cgtaacatct cgcagcagca tgaacagcaa
     3661 ttgcagatag cattgccaaa gcctttgttc tcggtcatat gcaccaggcc tgcttcctca
     3721 gaaaatttta gtatttcaaa agcttcctct ttggtaattg ccctgcctgt cccacgctcc
     3781 agtgtgtacg ttgcaccgtt gttgacctga atacatacat tgagtggttt gctgcagttt
     3841 ccatgggaaa ggcggcatac acaatcaact actgccagtt cctttgcctg ttcaattatt
     3901 ctgtatgcat cttcataaac cagtacttca ctttttggtt gaattgttgc attaattgga
     3961 attacccgca tgaaagacgg aatgttagct ttagtcacca cctctagcag tggcggatat
     4021 tcggtgttca taaattctac ccacagtttt ttaaaatcag cgggggcatc tctccacagt
     4081 agtgttgcat catggaactg gacaatatgt ttggggggtc ggtattctgc tccttcgggt
     4141 tttgattttt taaatgctgc acctcgctta aataattctt caagtatgcg agtagcctca
     4201 tccacggaaa ggttaaactt ttcagctacc tgggctgcat tagcaggcag ataacaaata
     4261 atctctgctt caatttcatt tgccacgcgc tgccagatct gttgtaaaag ctgagaattt
     4321 tcaaacataa gacgtttgga taattctcgg tagatactat ctgccatatt tcctcctgtt
     4381 atttttttac taattataac tagttactac atatatgtca aatatattat acagatagtc
     4441 attgtgaata tcgatagca
//
LOCUS       DAORYL010000139         4402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_13343_length_4402_cov_12.498275, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000139 DAORYL010000000
VERSION     DAORYL010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13343_length_4402_cov_12.498275"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="PLV81_14765"
     CDS             complement(<1..1107)
                     /locus_tag="PLV81_14765"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOM11400.1"
                     /translation="MKKIYYISYLLIFIILNEFVDTKLFCKNLLELYSDPVYSEEGLQ
                     NAINYNPDNDSIFLPKLEGKDIFTAINDLSICRIPDVRKFLYIYLTKGREYTITAIKR
                     SYLYMPLIREQMQHYEGIPEDIALLPLLESAFNPYAVSKSNAVGIWQFVPSTAKVLGL
                     QINDFVDERRDIIKSTNAALRHLNHLNKTFNSWELALLAYNGGCGYLSRTMKANGYHH
                     FYDLIESGVLRKETAEYIYRYAALALIYKYPELFSIEKDLLKPDNDYDIELVELRYPV
                     RINLLTKKCNIPSEVIKLYNPQLKRNITPPKERNYILLLPKGSKEKIEEYKDEIYTLK
                     FTSIYTHKVKSGETLSQIAKRYRASPKTIIMINNL"
     gene            1415..1981
                     /locus_tag="PLV81_14770"
     CDS             1415..1981
                     /locus_tag="PLV81_14770"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOM11401.1"
                     /translation="MDQLIENIGKKIQYFRKKNDITLKELADKIHATPSLISQIEHGK
                     ANPSLATLKAIADVFNVPIGLFFENEIKRTAPSPVIRKNTHRRLLTDGNVSYTLLNPD
                     SNEMEVILIEFPPGASTGEEHYHHDGYEVGYIIKGELTVALEDNEYNLQQGDSIAFKS
                     LRSHKIKNNSSHTTLALWVNLVPWIFVK"
     gene            1993..3108
                     /locus_tag="PLV81_14775"
     CDS             1993..3108
                     /locus_tag="PLV81_14775"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOM11402.1"
                     /translation="MTNTNTETKSTIYTKTKLKLSILHMVIELILLGVGAFTISKPLY
                     QLLSPLIKNDYVLLLAFFAAFGGLLSVILLPLEFYQSYIVEHKFGLSNQTVFDWCIEE
                     LKSLLVSIVIGLPLILLFYYLIKITPLWWLYFAIVVFIFAIILAKVAPVLIFPLFYKF
                     TPIDNDALKSSLTELMQKHGLYVAGIFSFNMSKDTKKANAAFTGLGKTRRIILSDTML
                     NEFSVNEIKTVFAHELGHYVYKHIIKNIVLSGIVIVVSFYVCSYLYEITLYSFGYSSR
                     FDIAALPLLIFYLSIFSLLLMPLLNTLSRYYEKQADVYALKVTGDPQSFISTMEKLAA
                     LNLSQKEQNPVIEFLLYSHPTIAKRIELAKNYRVPTS"
     gene            complement(3076..4101)
                     /gene="purM"
                     /locus_tag="PLV81_14780"
     CDS             complement(3076..4101)
                     /gene="purM"
                     /locus_tag="PLV81_14780"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOM11403.1"
                     /translation="MTYKDSGVDVEGGNRFVKRISSIVKETFTKEVLTDIGGFAALFD
                     ATFKQYKEPVLVSSTDGVGTKLKIAQMMNVHTTIGIDAVAMCVNDIVVTGARPLFFLD
                     YIACGKLNEDTLVDVIKGLATGCKMADCALIGGETAEHPNVMQPDDYDIAGFSVGVVD
                     RHRIIDGSTIKPGDVIVGIASSGIHSNGYSLVRKLLFDIKKYTVETKLNELSKPLGQE
                     LLEPTRIYVTPILKALATVTIKGLVHITGGGFYENIPRILPVTTAAYIEKKSFSVPPI
                     FQIIQREGNIEEKEMFTTFNMGIGMICVVAKDDAKSLVNILTQNGEKAYIIGHIQSRS
                     GNPVILG"
BASE COUNT         1425 a          727 c          746 g         1504 t
ORIGIN      
        1 gagattattg atcatgataa tggttttagg gcttgcgcgg tatcgctttg ctatttgtga
       61 caatgtttct cctgatttta ctttatgagt gtaaatggat gtaaatttaa gcgtatatat
      121 ttcatcttta tattcttcta ttttttcttt actacctttt ggcaataaca gtatataatt
      181 acgttctttg ggtggggtta tatttctttt tagttgagga ttgtaaagct taatgacttc
      241 agaaggaata ttacattttt tagtaagtag attaatcctt accgggtatc ttaattctac
      301 tagctcaata tcataatcat tatcaggctt aagtaaatcc ttttcaattg aaaatagttc
      361 ggggtattta taaataagtg ctaatgcagc gtaacgataa atatattctg cggtttcttt
      421 ccgtaagact cccgattcaa taaggtcata aaaatggtga tagccattgg ctttcatggt
      481 ccgtgataaa taaccacatc caccgttgta tgcaagaagt gcaagttccc atgaattaaa
      541 tgttttattc aaatggttga ggtgccgtag tgcagcattt gttgatttta ttatatcgcg
      601 acgctcatca acaaaatcgt ttatctgcaa ccccaggact tttgctgttg acggaacaaa
      661 ttgccatatt ccaactgcat tacttttgga aaccgcatag ggattgaaag cgctttcaag
      721 taatggaagc agcgcaatgt cttctgggat accttcatag tgctgcatct gttccctgat
      781 taaaggcatg tataggtatg aacgttttat tgcagtgatg gtatattccc tgcctttggt
      841 aagatagatg tacaaaaatt ttcgtacatc aggaattcta caaatagaca aatcatttat
      901 agcggtaaaa atatctttac cttctagttt tggtaaaaat atgctatcat tatcaggatt
      961 ataattaatg gcattctgta gcccttcttc tgaatatacg ggatcggaat ataattctaa
     1021 tagattttta caaaaaagtt tagtatcaac aaattcattg agtataataa aaatgagcaa
     1081 atatgatatg taatatattt ttttcatccg ctaaaatatc cctatgtttt tataaagtag
     1141 tatatataaa attaaatgaa caataaatta caaatagtaa ataaaaagta atcatgtatc
     1201 ataatagcaa aagtggaata atatgtcaaa aaaattgtac aaaaaaagat aagttgtact
     1261 aacattattg gtggctgtaa tatttaattg ttgtatgcaa taatacatga tttatatata
     1321 ttttaataaa caataaataa aaagtgatgg acattattca atgaaataat tatatttatc
     1381 agaatagttt acaacaagaa gaaggttgaa ttgtatggat caactcattg aaaatattgg
     1441 caagaagatt caatatttca ggaaaaaaaa tgatataacc ttaaaggaat tagctgataa
     1501 aatacatgca acgccaagcc ttataagcca gatagaacat ggaaaagcaa atccttcgct
     1561 ggcaacattg aaagcaattg ctgatgtatt caatgtaccc attggcttat tttttgaaaa
     1621 tgagataaaa agaacagcac cttccccggt tatacgaaaa aatacgcacc gaaggttgct
     1681 caccgatggg aatgtttcat atacattatt aaaccctgac tccaatgaaa tggaagtaat
     1741 cttgattgag tttcctccag gtgcttcaac aggtgaagaa cattaccatc atgacggata
     1801 tgaagtggga tatataataa agggtgaact tacagttgca ttggaagata atgaatacaa
     1861 tttacagcag ggagacagca ttgcatttaa aagcttgcgt tcgcataaaa taaaaaataa
     1921 ttcttcgcat acaacccttg ctctgtgggt taatcttgta ccgtggattt ttgtgaaata
     1981 gggaaaaatt ccatgactaa cactaacacc gaaacaaagt caactattta tacaaagaca
     2041 aagttaaagc tctctatact gcatatggtg atagaactta ttcttttggg tgttggtgca
     2101 tttacaatct caaagccact ttaccagcta ctatcgccct taataaaaaa tgattatgta
     2161 ttgcttttag ccttttttgc agcttttggt ggtttgttgt ccgttatact tttgcccctg
     2221 gagttttatc aaagctatat tgttgaacac aagtttggac tttcaaatca aacagttttt
     2281 gactggtgta ttgaagaatt aaaatctttg ctggtttcaa ttgttattgg ccttccactg
     2341 atattgttat tttattatct catcaaaatt actccgctat ggtggctgta ttttgctatt
     2401 gtagttttta tatttgcaat tatccttgca aaagttgctc ctgtattgat tttcccgtta
     2461 ttttataaat ttacacctat tgacaatgat gcattaaaaa gctccttgac tgaactcatg
     2521 caaaaacatg ggttatatgt tgcagggatt ttttcgttta atatgagtaa ggatacaaaa
     2581 aaagctaatg ctgcatttac cggtttgggt aaaacacgaa gaataatctt aagcgatacc
     2641 atgctcaatg aatttagtgt taatgaaatc aaaacagtat ttgcccatga gctggggcac
     2701 tatgtttata agcatattat aaaaaacatt gtgctaagtg gtattgtaat tgttgtatct
     2761 ttttatgtgt gttcttatct atatgaaata acgttataca gttttggata ttccagcaga
     2821 tttgatattg cagctttacc actattaatt ttttatcttt caattttcag cctgctactt
     2881 atgccactgc ttaacactct gtcgcggtac tacgaaaaac aggctgatgt atatgcactt
     2941 aaagtaaccg gtgatccaca gtctttcata tcaacgatgg aaaagttagc ggcactgaat
     3001 ctgtcacaaa aggaacagaa tccggtaatt gagtttcttc tgtacagcca cccaacaatt
     3061 gcaaagcgaa ttgagttagc caagaattac agggttccca cttcgtgatt gtatatggcc
     3121 aataatatat gctttttcgc cgttttgtgt taaaatattt accagtgatt ttgcatcatc
     3181 ttttgctaca acacatatca tgccaatacc catattaaat gtggtgaaca tctctttttc
     3241 ttcaatgttg ccttcacgct gtattatctg aaatattggt ggcacgctaa atgatttctt
     3301 ttcaatgtat gcagcggttg taactggcaa aatgcgcggg atattttcat aaaagccgcc
     3361 acccgttata tgaaccagcc ctttaatggt aactgtcgcc aatgctttta aaatgggtgt
     3421 aacatatatg cgtgttggtt caagcaattc ctggcctaat ggttttgata attcattaag
     3481 ttttgtttca acagtatatt tcttgatatc aaacaataat ttcctgacaa gagaatatcc
     3541 attagaatgg attccagatg aggcaatgcc tacaattaca tcaccaggtt taatagtact
     3601 tccatcgata atgcgatgcc tatcgacaac accaacactg aatcctgcta tgtcataatc
     3661 atcaggctgc ataacatttg gatgctcagc tgtctccccc ccaataagtg cacaatctgc
     3721 cattttacaa cctgtggcca atccctttat aacatcaact aatgtatctt cattgagttt
     3781 tccacaggct atgtaatcaa gaaagaatag tggcctggca ccggttacaa cgatatcgtt
     3841 aacacacatt gccacagcat caatgccaat ggttgtgtgt acattcatca tctgtgcaat
     3901 ctttaatttt gttcccacac cgtcggtact ggaaacaagt acaggttctt tatattgttt
     3961 gaatgtagcg tcaaataatg cagcaaatcc tccaatatcg gttaaaactt cttttgtaaa
     4021 tgtttctttt actatgctgc tgatgcgttt tacaaacctg ttgccacctt caacatcaac
     4081 tcctgaatct ttgtatgtaa tgcccatgat tacctctttg aaatagtgta gggtgaatgg
     4141 tcgatagtga attgtaaatt attattggta aataataata actattcaaa aaaccagcta
     4201 tggttatttg tcaataaaga aaactaaaaa gatggttgga agatgcaagt aagtatgaat
     4261 taatgctgta aactgtgcaa tagtaaatag tatgtatact gtaaatgatt aatcatcaac
     4321 tattcacaat ccataatatt ttattattat acataatttt atatataatt tattgcttgc
     4381 aattaatttg tatatattat ca
//
LOCUS       DAORYL010000140         4262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_13916_length_4262_cov_12.347991, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000140 DAORYL010000000
VERSION     DAORYL010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13916_length_4262_cov_12.347991"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..376
                     /locus_tag="PLV81_14785"
     CDS             11..376
                     /locus_tag="PLV81_14785"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOM11404.1"
                     /translation="MSSKQITYIVTHADDDPEMASIPFVLANTAVAMDVTPVIILQGK
                     AVMLAMKGYAEHIHFEGFNPLMDMIKSYVEAGNKLLVCTPCMNKRKIKVEDLIEGAEA
                     IGGAKVNEIVLGSDAVLTY"
     gene            complement(384..1622)
                     /locus_tag="PLV81_14790"
     CDS             complement(384..1622)
                     /locus_tag="PLV81_14790"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOM11405.1"
                     /translation="MNKKTLYFLTEYALTIVETIIEVLGPRPSCSNNSHITARILEIL
                     YSAYCSSSQLQSFTAHPAAFLGFLTLVPWIYITSLIFLLLHYYGIAALGFTVANIIAS
                     HQFIFYKGTFDFLYKPKKGYNAIGIINPKKEVRQQIIISGHHDSAYEFRYMRTTPRLY
                     RIRVASIILSMVLSLVIGWLIVVNAYWFQSHTAHIVFMVIIVLLGIANMQMLFFKSKN
                     ATPGAGDNLIASAITAVMAKIFSNYRPDYTRLIFVSFDGEESGLKGSKAFAKAYHECI
                     GSMPTYHFNMDSLYKPELIKFLTSDVNGFVKLSNEFAKQCVTIAHSKGYTATLFAMYP
                     GTGATDAAPLAQAGAIATTIIALPTEVEKQDSVYHTMNDTTEHIEPEVVQATLDILYS
                     VIEFLDKEIKGCPQILIKHQ"
     gene            1859..2206
                     /locus_tag="PLV81_14795"
     CDS             1859..2206
                     /locus_tag="PLV81_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015414442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOM11406.1"
                     /translation="MNPLVKQKYETRAAIMKALAHPSRLMMVEELEKGPRCVCELQEL
                     VGADMSTVSKHLAVMKNAGLVYDVKQGTTVYYHLKTSCVLDFIACVDRVIEHNVAHQV
                     KIARCCVRSVRHS"
     gene            2283..3581
                     /locus_tag="PLV81_14800"
     CDS             2283..3581
                     /locus_tag="PLV81_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOM11407.1"
                     /translation="MDWKSELKKLLYIVAAFLIFYYLPIGNPRFDNAVFEALHLTKWY
                     AQEHVLLCLVPAFYIAGAIAAFVSQNSVMKYLGPTAPKLLAYGVASVSGTILAVCSCT
                     VLPLFAGIYTMGAGLGPATAFLYSGPAINVLAIILTARVLGLEIGVARAVGAILFSVV
                     IGALMYLIFRKEEIEKANAMMNILKPETPRPLWQTVVYFFLMVAILVFANWGKPATNE
                     GIWYTIFSYKWEITGISSLLFGLTLGAWFGVKWWKVLVSIIVVGVLGFLFQHEPMIPF
                     AAGVVALTLITATEKGEMTTWLEQTWSFAKQILPLLLGGVLVAGFLLGRPGHEGIIPS
                     EWVSVAVGGNSVFANFFSSLVGAFMYFATLTEVPILQGLLGSGMGYGPALALLLAGPA
                     LSLPNMLVIRSVIGTKKTVIYVLLVVIMATITGLIFGMIY"
     gene            3598..>4262
                     /locus_tag="PLV81_14805"
     CDS             3598..>4262
                     /locus_tag="PLV81_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11408.1"
                     /translation="MLRKIVMVFIVFAIVTVHVFADENKLPVTISGVGYLEWQKDIQK
                     SDETENENTFAITRVYLNFTKKFDEIWSMRITTDIGQVESEGETSNNNDDVKTKTNNY
                     DVYLKFAYIQAKQDFGAIGYCLRFGMVGTPLLGYLDEQSDYRWIQRNYFDNAKDLIGT
                     SVDYSADAGVRADVSLFKMVAITGAITNGEGFKHVSETDDGKAYYGMLTVNPFEHFSL
                     IAF"
BASE COUNT         1243 a          709 c          960 g         1350 t
ORIGIN      
        1 aggaggatgt atgagcagta aacagattac gtatattgta actcatgctg atgatgatcc
       61 cgaaatggct tccattccgt ttgttcttgc aaacacagca gtagcaatgg atgtaacacc
      121 ggttatcatt ttgcagggta aagctgtcat gcttgcaatg aaaggatacg ctgagcacat
      181 ccattttgaa ggctttaatc cactaatgga tatgatcaaa tcatatgttg aagcaggcaa
      241 taaacttctt gtttgcacac cgtgcatgaa taaaagaaaa attaaagtag aagatttaat
      301 tgaaggtgct gaagctattg gtggtgcaaa agtaaatgag attgtccttg gaagtgatgc
      361 ggtcttgacc tactaaatga aaattattga tgcttaatta aaatttgtgg gcaaccttta
      421 atttccttat caaggaattc aataacactg tatagtatat caagagttgc ctgtacaact
      481 tctggttcaa tgtgctctgt agtatcattc atggtgtggt acacactatc ctgcttttca
      541 acttcagtgg gaagtgcaat aattgttgtt gcaatggcac cagcttgtgc cagtggtgca
      601 gcatcggtgg cgccggttcc cggatacatg gcaaatagtg tggccgtgta ccctttgcta
      661 tgagctatag ttacacattg ttttgcaaat tcgtttgaaa gcttgacaaa accattcaca
      721 tcacttgtca aaaatttgat gagctctggt ttataaaggc tatccatatt aaaatgatat
      781 gtgggcatgc tgccaataca ctcatggtat gcttttgcaa aagcttttga acctttaagt
      841 ccactttctt caccatcaaa gcttacaaag attaaacggg tatagtctgg gcgatagtta
      901 ctgaagattt ttgccatcac tgcggtaata gcacttgcta ttaagttatc gccagcacct
      961 ggcgtagcgt ttttgctttt gaaaaaaagc atctgcatgt tggcaatacc aagcagtaca
     1021 atgattacca tgaagacaat gtgtgcagtg tgtgattgaa accagtaagc atttacaaca
     1081 attaaccatc caataacaag cgatagtacc atggaaagaa tgattgatgc aacacgtata
     1141 cggtacaggc gcggtgtggt gcgcatataa cggaattcgt atgcactatc gtgatgaccg
     1201 ctaataatga tttgctgacg cacttctttt ttaggattga taatgccaat agcattgtag
     1261 cctttttttg gtttgtataa aaaatcaaag gtgcctttat aaaaaataaa ttgatgtgat
     1321 gcaatgatat ttgcaacagt aaatccaagt gcagctatac catagtagtg cagtaataga
     1381 aaaatgagtg aagtaatgta tatccatggc acaagcgtta aaaagcctaa aaaagcagca
     1441 ggatgtgcag taaatgattg aagctgtgaa gagctgcaat aagcactgta tagaatttct
     1501 aaaatgcgtg cagtaatgtg cgaattattg gagcaggatg gtctcgggcc aagaacttca
     1561 attatggttt caacaattgt caatgcatat tcagtcagaa agtagagtgt ttttttgttc
     1621 atggttcctc tcatattcag gtacaatgca agagcttttt ttacgcttag ttttacttaa
     1681 ttttgtgcag atgtatgcac agtaactatc gcacatatac gtaccgtaca tggaactata
     1741 ttgcaataaa attttaatga aaaatgtttg cactaaaaat tattttttta gttttaatat
     1801 ggatatttgg ctatattacc aaataagcaa aattaaaaat attctataag gtataagtat
     1861 gaatccatta gtaaagcaaa agtatgaaac acgtgctgct atcatgaaag cgctggcgca
     1921 tccaagcagg ctcatgatgg tagaggagct tgaaaaaggg cctcgatgcg tatgcgagct
     1981 tcaagagctt gttggtgctg acatgtcaac ggtgtccaaa cacttagcgg tgatgaaaaa
     2041 tgcagggctg gtgtatgatg taaaacaggg aaccactgta tattatcatc ttaagacttc
     2101 ctgtgttctt gactttattg cgtgtgttga tagggttatt gaacacaatg tggcacatca
     2161 ggttaagatt gctcgctgct gtgttcggag tgtacggcat tcatagaatt accattcatg
     2221 tacgctacaa atattatgac agttttattc atgtaacata caatatatta ggagatgcat
     2281 ttatggattg gaagagtgaa ttaaaaaagt tgctgtatat tgttgcagca tttcttattt
     2341 tttactatct gccaattggc aatccgcgtt ttgacaatgc tgtgtttgaa gcgttgcatc
     2401 tgacaaagtg gtatgcacag gaacatgttc ttttgtgcct ggtgcctgcg ttttatattg
     2461 caggagctat cgctgcattt gtaagccaga atagtgttat gaaatattta gggcctactg
     2521 cgccaaaatt actggcctat ggtgtggcat cggtttccgg tacaatactt gctgtgtgtt
     2581 catgtacggt gttgccgctt tttgcaggta tctatacaat gggagcagga ctgggacctg
     2641 caacagcctt tttatattca gggcctgcaa tcaatgttct ggcaattatt ttaactgccc
     2701 gtgtcctggg acttgaaatt ggtgttgcac gggctgtagg tgcaatttta ttcagtgttg
     2761 ttattggtgc attgatgtac ctgatattca ggaaagagga aatagaaaaa gccaatgcca
     2821 tgatgaatat actcaagcct gaaaccccgc gtccactgtg gcaaacggta gtctatttct
     2881 ttttgatggt ggcaatactg gtatttgcaa actggggaaa gcctgcaaca aatgaaggta
     2941 tatggtatac tattttttca tataaatggg aaatcaccgg catatcatcg ttgttatttg
     3001 ggctaacgct tggtgcatgg tttggtgtta aatggtggaa ggttctggtt tctatcatag
     3061 tcgttggtgt tttgggattt ctttttcagc atgagcccat gataccattt gctgcaggag
     3121 ttgttgcact gacacttatt actgcaactg aaaaaggtga aatgaccacc tggcttgagc
     3181 agacatggtc atttgcaaaa cagatactgc cgcttctttt aggtggtgta cttgttgctg
     3241 gatttttgtt gggtcgtcca ggacatgagg gaatcatccc gtcagagtgg gtaagtgttg
     3301 ctgttggtgg taattctgta tttgcaaact tcttctcttc actggttggt gcattcatgt
     3361 attttgccac actcaccgag gttcctattt tacagggatt acttggcagt ggtatgggat
     3421 atggtcctgc actggcactt ctattagcgg gtccagcgtt atcgcttccc aatatgcttg
     3481 taatacgcag tgtcattggt acaaaaaaga ctgttattta tgtgttgctg gttgtgatca
     3541 tggcaacgat cacgggactt atttttggaa tgatatatta aaaggagaat aacgtttatg
     3601 ctaaggaaga ttgtcatggt ttttattgtc tttgcgatag ttactgtgca tgtgtttgca
     3661 gatgagaata agcttcccgt aactatctca ggtgttggat accttgaatg gcaaaaggat
     3721 attcaaaaat cagatgaaac tgaaaatgaa aatacatttg ctattacccg ggtgtatctt
     3781 aattttacaa aaaaatttga tgagatatgg agcatgcgca ttaccactga tattggacag
     3841 gtggaaagtg aaggagaaac ttctaataat aatgacgatg ttaaaactaa aacaaataat
     3901 tatgacgtct atttaaaatt tgcctacata caggcaaaac aggattttgg tgcaattggc
     3961 tattgcctca gatttggcat ggtgggaaca ccgctacttg gatatcttga tgagcagagt
     4021 gattaccgct ggattcagcg taactacttt gataatgcaa aggatttaat aggaacttct
     4081 gttgattatt cggctgatgc tggggtcaga gccgatgttt ctttattcaa gatggttgcc
     4141 ataacaggag ctatcaccaa tggagaagga tttaagcatg tcagtgaaac tgatgatggt
     4201 aaagcatact atggtatgct taccgttaat ccgtttgaac atttcagcct tatagcattc
     4261 aa
//
LOCUS       DAORYL010000141         4253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_13959_length_4253_cov_13.749643, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000141 DAORYL010000000
VERSION     DAORYL010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13959_length_4253_cov_13.749643"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PLV81_14810"
     CDS             <1..708
                     /locus_tag="PLV81_14810"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOM11409.1"
                     /translation="KLLSECILHDMVFAEKRIERLNQDKKKTAQQKEKEIMLLQTFMQ
                     HLEQGNLLNSLKLADDDIAMIKSYPFITLKKIIVVFNTDDSGSEILDAFNRVYANHTV
                     KAVAVAVKLESEISLIDDEKEQKEFYASMGVEKPALHVLCDAMLDSLGLQSFFTVGQD
                     EVKQWLIPKGITAPLAAGYIHSDIQRGFIRAELMHYDDFIVAGSEERLKKEGKYHLVG
                     KDYYMQDGDIVSFRFNV"
     gene            797..1690
                     /locus_tag="PLV81_14815"
     CDS             797..1690
                     /locus_tag="PLV81_14815"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOM11410.1"
                     /translation="MDKLLSGLARVRESYRTKKNIDATIKKKFLSCIYEFYDRNYRHL
                     PWRNTDNPYHILVSEVMLQQTQVSRVIEKYSTFIKKFPTVETLAQASLHDVLATWQGL
                     GYNRRAKYLWQAAQIIVSQYNGTVPDNHHALHKLPGLGKATTASVAIFAFGRSELYIE
                     TNVRTVYIYSFFADNEKVSDAMIEPLLKATIDTTNPKKFYNALMDCGTYIKQHYNFSR
                     HSAHYTVQTPYSTSLRKVRGDIVRYIVNNGAANNDMLVQSLGYAPEKIQKALEALCRE
                     NMVCEDNGIYTIPSRELIKNN"
     gene            1771..2580
                     /locus_tag="PLV81_14820"
     CDS             1771..2580
                     /locus_tag="PLV81_14820"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HOM11411.1"
                     /translation="MIKRLALLFVLVFVLCTSANVFAVPVAYRSWTYLDLTALLSPQW
                     SYTLLTSHSYEYERKNDPGKEEKKTFFYEVFTGPTYTMRFDNLTVKLPLWYYYQSFPT
                     RAAVSPTGKDEHYYSHNIEFLPIIEYKIGNLKLWNRIIFHNKVYSTYPSYTTSSLKKG
                     YSLLIREYFRIEYSITDQFAVMIGDEIFQGIIDDEDTKDITKAPLTPGFEYGGFSQNR
                     LYVGFSYKFTPTIILTPMYMYQTQYFKTGNKLTEKDHYLFTTLTFLFKTYE"
     gene            2720..3493
                     /locus_tag="PLV81_14825"
     CDS             2720..3493
                     /locus_tag="PLV81_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11412.1"
                     /translation="MKFETLQQLLEYDASLHALIEPDSRFAMVFDRLKDCIETYKPHV
                     IVKIGLGNPKYIEAIMQLSKALCVVVEPSLPLLQSFYARSGTQEWMNRLYTIAGNFND
                     FPIDYYKADMVVCIDCLDLLDTGRAIDEFRRALQFEGVFFLAGFVLPNEDIEGIFDEI
                     AHTINPLHTDYYLEDDLKTFLTLNEFGLAKSHTEKVTVNLFSVAEYLGTSNQQLQQLI
                     DENKELLSNIYMLQNNETVTLPYMISAYIRQKPSSDETI"
     gene            complement(3501..>4253)
                     /locus_tag="PLV81_14830"
     CDS             complement(3501..>4253)
                     /locus_tag="PLV81_14830"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOM11413.1"
                     /translation="LNLEYCFPFVADYVKPKLTIIDGIYALEKGALHFGNAYKKDIII
                     ASTDILAADMVGAKVMGYDPTDIAHFVTYAERHKKSLSLQDYEIKGEKLEDHIQPLKW
                     DWAWTEDNTGPGVFAKMGVSGVALPKYDDTLCSGCSPIANMCNILVLSAFKGQPLPKV
                     EILNGKKMQARPGYDKTILLGNCIIKANKNNPNIKEPVEVKGCPPDFEDVVKTLKSCG
                     LEVNEMAYLGYMKQQSEKYNGKEGYDPAFYKA"
BASE COUNT         1332 a          764 c          845 g         1312 t
ORIGIN      
        1 aagctactga gtgaatgtat tttgcatgat atggtgtttg cagaaaagcg gatagaacgt
       61 cttaatcagg ataaaaagaa aacagcgcaa caaaaagaaa aagaaattat gttactgcaa
      121 acatttatgc agcatctgga acagggcaat cttttaaaca gtctgaagct tgctgatgat
      181 gatattgcaa tgattaagag ctacccgttt attacattga agaaaataat agttgttttc
      241 aatactgatg atagcggcag tgaaattcta gatgcattta atagagttta tgcaaaccat
      301 accgtgaagg cagtagctgt tgcggtaaag cttgaaagcg aaatatcgct gatagatgat
      361 gaaaaagaac aaaaagaatt ttacgcttcc atgggtgtgg aaaaacctgc attgcatgta
      421 ttatgcgatg ctatgcttga ctctcttggc ctgcagtcgt tttttactgt tgggcaggat
      481 gaagtaaaac agtggcttat ccccaaaggt atcacagcgc cacttgcagc aggctatatc
      541 cacagtgata ttcagcgcgg cttcatcaga gctgagctta tgcattatga tgattttatt
      601 gtagcaggtt ctgaggagcg ccttaaaaaa gaaggaaagt atcacctggt tggcaaagac
      661 tactatatgc aggatggtga tattgtgtca ttcaggttta atgtgtagcg atagtcatgg
      721 tgtatgaact attactttta accatagcca ttgtctacat tttaaaaacg gcatattaat
      781 tctggcgata gttactatgg ataagcttct ttcaggactt gcacgggtac gggagtccta
      841 tcgaacaaaa aagaatattg atgcaacaat aaaaaagaag tttttatcat gtatatatga
      901 attctatgac aggaactatc gccacctgcc ctggcgaaat actgataatc cctaccacat
      961 tcttgtttcc gaagtgatgc tacagcaaac gcaggtgagc cgcgttattg aaaaatattc
     1021 tacatttatc aaaaaatttc caactgtgga aactctggca caggcaagcc ttcatgacgt
     1081 tttagctaca tggcagggat tggggtataa caggcgggca aagtatctgt ggcaggctgc
     1141 gcagatcatt gtgtcacagt acaatggcac ggtgccagat aatcaccatg cattacacaa
     1201 acttcctggt ctggggaagg caactactgc ttcggttgcc atatttgcat ttggcagatc
     1261 tgaattgtat attgaaacca atgtgcgcac agtatatatt tatagctttt ttgctgataa
     1321 tgaaaaagta tctgatgcaa tgatagagcc tttattgaaa gcaactatcg atactactaa
     1381 tccaaaaaaa ttttataatg cacttatgga ttgtggcacg tatataaagc agcactataa
     1441 tttttcgcgt catagcgcac attacacagt acaaacgccg tacagtactt cattgcgaaa
     1501 agtgcgcggt gatatagttc ggtatattgt aaacaatggt gcagcaaata atgatatgct
     1561 ggtgcaaagc ctgggatatg cgcctgaaaa aatacagaaa gcacttgagg cgctgtgccg
     1621 ggaaaacatg gtatgcgaag ataacggcat atatacgatt cccagccgtg agttgataaa
     1681 aaataattga caaaatgttt acaatgtcga ttgactataa tgaggtatta gtttaattcc
     1741 atattatttt tacacacaga gaggtttccc atgataaaac gacttgcttt attattcgtg
     1801 ctggtgtttg ttttgtgtac atcagcaaat gtatttgctg taccagttgc ataccgttcg
     1861 tggacgtatt tagatttaac ggcattgctt tcaccacagt ggtcatatac tctgttaaca
     1921 agccacagct atgagtatga aagaaaaaat gatccaggta aagaagaaaa aaagacattc
     1981 ttttacgaag tatttacagg tcccacctat accatgcggt ttgataattt aactgttaaa
     2041 ctgccattat ggtattacta tcagagcttc ccaaccaggg cagcagtatc gcctaccggt
     2101 aaggatgagc attactattc acataatatt gagtttttgc ctattattga atataaaatt
     2161 ggaaatttga aattgtggaa tcgaataatt ttccataata aagtgtattc cacctatcca
     2221 tcttatacaa caagttcact taaaaaggga tattcattac ttattcgtga atatttcaga
     2281 attgaataca gcattactga ccagtttgct gttatgattg gtgatgaaat attccagggc
     2341 atcatagatg atgaggatac aaaagatata acaaaagctc ctcttacacc tggttttgaa
     2401 tatggtggat tttcacagaa caggctatac gttggtttca gctataagtt tacaccaacc
     2461 ataatactta ctcccatgta catgtatcag acacagtact ttaaaactgg taataagtta
     2521 actgaaaaag accactacct gtttacaaca ctgacattcc tctttaagac gtatgaataa
     2581 atacatgcta tacatagctg taatgaaaaa tcccacggat tactctgtgg gattttttta
     2641 tgcttgcatt tttattatca tggattaatt atgataaggt aaaatactac aaaaaatata
     2701 ttacagcggg aaagaaagca tgaaatttga aacgttgcag cagcttttag aatacgatgc
     2761 atcattacac gcattaattg aaccagatag tcgctttgcc atggtttttg acaggctcaa
     2821 agattgtatt gaaacatata aaccgcatgt catcgttaaa attgggctgg ggaatccaaa
     2881 atatattgaa gctattatgc aactttcaaa agcactatgt gtggtggttg agccttcgct
     2941 tccattgttg caatcgtttt atgccaggtc tggcacacag gaatggatga atcgtctgta
     3001 tacaattgca ggaaatttca atgatttccc cattgattac tataaggctg atatggttgt
     3061 atgtattgat tgcctggatt tacttgatac agggcgggca attgatgaat tcagacgtgc
     3121 tttacagttt gaaggcgtgt tctttttagc gggatttgtg ttgcccaatg aagacataga
     3181 aggaatattt gatgagatag ctcataccat aaatcctctt cataccgatt actatctgga
     3241 agatgactta aagactttct taacactcaa tgagtttgga ctggcaaaat cccatacaga
     3301 gaaagttact gttaaccttt tttcagttgc agaatacctg ggtaccagta atcagcagtt
     3361 gcaacaattg attgatgaaa acaaagaatt gctttctaat atctacatgt tacagaataa
     3421 tgaaacagtg accctgccgt atatgatttc tgcatatata cggcagaagc cttcatcaga
     3481 tgaaacaata taatttctga tcaggcttta tagaatgcag ggtcatatcc ttctttgcca
     3541 ttatattttt cgctctgctg tttcatgtag cctaagtatg ccatttcgtt aacttcaagt
     3601 ccgcaggatt ttaaggtttt caccacatct tcaaaatcgg gtgggcaccc ttttacttca
     3661 actggctctt tgatgttggg gttgttttta tttgctttga ttatacagtt gccaagcaat
     3721 attgttttat catagccagg acgcgcctgc atctttttgc cattgagtat ttcaaccttt
     3781 ggcagtggtt gtcctttaaa tgctgataat accagaatat tgcacatatt ggcaattggg
     3841 gaacatccag agcacagcgt atcatcatat tttgggagag caacaccact tacacccatc
     3901 tttgcaaaaa ccccagggcc agtattgtct tcggtccatg cccagtccca tttcagtggc
     3961 tgtatgtggt cctccagttt ttcacctttt atttcgtaat cctgtaatga taatgacttt
     4021 ttatgacgct cagcgtaagt tacaaaatga gcaatatcag ttggatcata tcccataact
     4081 ttggccccaa ccatatctgc agcaagaata tcggtagatg ctatgataat atcttttttg
     4141 taggcattac caaagtgcag tgcacctttt tccagagcat agataccatc aatgattgtt
     4201 agctttggtt ttacgtaatc agcaacaaat gggaagcaat attctaaatt aag
//
LOCUS       DAORYL010000142         4210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_14151_length_4210_cov_12.302286, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000142 DAORYL010000000
VERSION     DAORYL010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14151_length_4210_cov_12.302286"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="PLV81_14835"
     CDS             complement(<1..896)
                     /locus_tag="PLV81_14835"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOM11414.1"
                     /translation="MKKLFFLILGCCIILSCSDSFIENYKTELLKKRAEGKTFALVTG
                     TTIISFTEFDPLGGTEQPGIFKAKLSAPPESDVVLDLTKADQLCTTPGIEETLQFNPT
                     TLVFTPENYNQEQEVEITVSDNMLVNETRGHRVILPPLESSDPLYNGVETPTLYVTVF
                     EDDTRPIAYPVTPYIGQINVPEHQAIIVTFNREMDEGSIHENSFKVTNERGEIVSGYM
                     WVGSDKKTAYFTPKQYLLNGSLYTVDINKSIKDTYGNELISGLSWEFKARLLNLTLTK
                     SCDTNGTALGVAVVGNYAYVADG"
     gene            complement(920..3013)
                     /locus_tag="PLV81_14840"
     CDS             complement(920..3013)
                     /locus_tag="PLV81_14840"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM11415.1"
                     /translation="MKKIVLGIIILAVTFPCILYAADPLEIFSLGLFGGYFLPSGYYA
                     DIFNQGSGFGLSAMADTKWRNVFLDATALVAIYSMKNSTNSTLQTYTLSAGPGYMFTI
                     TPWLKPYISVQGGMAYMRFNFDQSGVSSSTWKPLGIGTAGIIVAPFEYVSLRLGASYS
                     VSELSEKKLYVAQFTGSAIMHLSVFAARNIVDIKESLVRLTDIQLKPIWGARYALYQS
                     EYIGSVKVTNYSKETLRDIRVETAIEDIASGPTNSETVNILEPGKSVELDLPLSINQK
                     ILDVTGTRELPVKLRTFYTSSKGVFSYIEKKNVTVHSKNYLTWNNTAYIGSFITPQEE
                     TVSSFVRKSVSMYKQKMLPGFNKKLQEALVIFDSLGALGVTYATDPNTGYGKMDASSI
                     DFVMFPRETLRKKAGDCDDLTVLYCALLENLGIKTAVVTVPGHIFMMFDTEVPENSYT
                     DISADSSLIYTLNGTVWIPVEVTMVGKSFLAAWQEGSKNMKKYGGMQGKFGILETSVA
                     WNKFPPADIGTGSTISIPSQEKIDMLYTLDMDELKTKGFEEPVQLLKAQLGSANEYDA
                     LNKLGVLYAKHGILDQAISYFQKAVSTYPDRAPAYVNLGNVYMMKKDYTKALPLYQKA
                     VELNPNNHKYRLSLARALFETGKPYKAKDEYQQALKNNPGYARRYAYLESNPKEKAAD
                     PDERAGYNMWIVEGK"
     gene            complement(3017..3877)
                     /locus_tag="PLV81_14845"
     CDS             complement(3017..3877)
                     /locus_tag="PLV81_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11416.1"
                     /translation="MKIRFFFLFLILFFPFTLLHAEQRLNFFIGDVKIEVNGKNVEPE
                     IGMVLPPNAVIITGKRSQAHIFDTVSGIVTSIQPQQKITVVSLAPTKQNKNLTVWDKI
                     RKEKHTTGIVTTAAVRGAEEGNVELEWDTVNEESTMKNDRATEWMLFGEKQYDRVISM
                     TKNASDMDGVFLNAASTYYSKGMNKAEKVIPVLKQIIAGSAKNIIKSESNKILAAIYF
                     EQGRFDLAWEHMNAAAVTIPENEISETGYYILAQSAFFTNRSDEGHLYLKKMKKYYKD
                     SPLIEKVIER"
BASE COUNT         1194 a          912 c          748 g         1356 t
ORIGIN      
        1 ccatcagcta catacgcata attacccacc accgcaacgc ctagggcagt accattggta
       61 tcacagcttt ttgtcaatgt tagatttaat aatcttgctt tgaattccca gcttagcccc
      121 gatatcaatt cattgccgta tgtatcttta atggatttat ttatatctac cgtatataaa
      181 cttccattca acaaatattg ctttggtgta aaatatgctg tctttttatc gcttccaacc
      241 cacatatagc cactaactat ttcaccacgt tcatttgtta ctttgaaact attctcatga
      301 atgctaccct catccatctc gcggttaaag gtcactataa tggcttgatg ctctggaaca
      361 tttatttgcc caatataagg tgttaccgga tatgcaattg gcctggtatc atcctcaaac
      421 accgtaacat acagggtagg tgtttccact ccattataca gggggtcaga gctttcaagc
      481 ggtggcagta ttacccggtg accacgggtt tcattcacta acatgttatc gctaaccgtt
      541 atctcaactt cctgttcctg gttatagttt tctggagtaa acacaagtgt tgttggatta
      601 aattgcagtg tttcctcaat acccggagtg gtgcaaagct gatcagcttt tgtaaggtca
      661 agtacaacat cggactccgg tggagcagaa agctttgcct taaaaattcc aggctgctct
      721 gtaccaccaa ggggatcaaa ttccgtaaaa cttatgatgg tggtgcctgt taccagagca
      781 aaggtcttcc cttctgctcg cttttttaat agctcagttt tataattttc aataaaacta
      841 tcgctacagg aaagtataat acaacatcca agtattaaaa aaaatagttt tttcataata
      901 tttttccttg cctattgtgt tatttccctt ccacaatcca catattatat cctgcccgct
      961 catcaggatc ggcagctttt tctttggggt tactttctaa atatgcatag cgccgtgcat
     1021 aaccgggatt gtttttaagc gcctgctgat attcatcttt tgctttataa ggctttcctg
     1081 tttcaaagag agcacgtgct aagctcagac gatatttgtg attgtttggg ttaagctcaa
     1141 ctgctttctg gtatagtggc aatgcctttg tataatcctt tttcatcatg taaacattac
     1201 ctaagtttac atacgctggg gcacggtcag gatatgtact gacagctttt tgaaaatagc
     1261 tgatagcctg atcaagtatc ccatgtttag catacagtac tcctaattta ttgagtgcat
     1321 catattcatt tgcactacca agttgcgctt taagcaactg tacaggttct tcaaaacctt
     1381 ttgtctttag ctcatccata tctaaagtat acagcatatc aatcttttcc tgtgatggga
     1441 ttgaaatggt gcttcctgtt ccaatatctg ctggggggaa cttattccat gctacagaag
     1501 tttcaagaat cccaaatttt ccttgcatgc caccatactt tttcatgttt ttggatccct
     1561 cctgccaggc agctaaaaat gattttccca ccatggttac ttccacaggt atccacaccg
     1621 taccatttaa tgtgtatata agggaactat cggctgatat atcagtataa ctgttttctg
     1681 gtacttcagt atcaaacatc atgaagatgt gaccaggtac ggttacgact gctgtcttta
     1741 tacctaaatt ttcaagcaac gcacagtaaa gaacagttaa atcatcacag tcaccggctt
     1801 ttttgcgtaa tgtttctcgt gggaacataa caaaatcaat ggatgatgca tccattttgc
     1861 cgtagcctgt atttgggtct gtagcatagg taacacccaa cgctccaaga ctatcaaaaa
     1921 taaccagtgc ctcctgcagt tttttattaa atccgggcag catcttttgc ttatacatag
     1981 aaacagattt ccttacaaat gaagaaactg tttcttcctg tggcgtaata aagcttccta
     2041 tgtatgctgt attgttccat gtgagatagt tcttggaatg aaccgtcaca ttctttttct
     2101 caatgtatga gaatacgcct tttgatgatg tgtagaaggt acgcaatttc actggtagtt
     2161 cacgagttcc agtcacatct aaaattttct ggttaattga caaaggaaga tctaactcta
     2221 cacttttccc tggctcaaga atattaaccg tttcagagtt tgtgggacca cttgcaatat
     2281 cctcaatggc agtttctacg cggatatccc ggagtgtttc tttactgtag tttgtcactt
     2341 tcacactgcc tatatattca gactggtaca gtgcataccg tgcaccccag ataggcttaa
     2401 gctgtatatc agtaagcctt accagtgatt cttttatatc cacaatattg cgtgctgcaa
     2461 aaacactcag gtgcatgatt gctgaacccg taaattgggc aacatatagc ttcttttctg
     2521 aaagctcact tacactatag cttgctccaa gacgtaagct gacatactca aagggagcta
     2581 ctattattcc tgcggtgcca atgcccagtg gcttccaggt tgatgacgaa acaccactct
     2641 ggtcaaagtt aaagcgcata tacgccattc caccctgaac gcttatataa ggtttcagcc
     2701 atggggtaat ggtaaacata taccccggtc cagcagataa agtgtatgtc tgcaaagtgg
     2761 agttagtgct attcttcata gaataaatgg caacaagagc ggttgcatcc aggaaaacat
     2821 tgcgccattt tgtatcagcc atggctgaaa gcccaaaacc gctaccctgg ttgaatatat
     2881 ctgcataata tccagatgga agaaaatatc ctccaaataa tcccaatgaa aagatttcaa
     2941 gaggatcagc agcatacagt atacagggaa aagtaacggc cagaataatt attcccagta
     3001 caatcttttt catggctcac ctctcaataa ctttttcaat gagcggggag tctttataat
     3061 actttttcat ttttttcagg tataagtgcc cttcatcaga gcggtttgta aaaaatgcac
     3121 tttgagccaa tatgtaatac cctgtttcac tgatctcatt ttcaggaata gtaacagcgg
     3181 cagcattcat atgctcccag gctaaatcaa accgcccctg ttcaaaatat attgctgcaa
     3241 gtattttgtt actttcactt ttaatgatgt tttttgcact cccagcaata atctgcttca
     3301 atacaggaat tactttttct gctttgttca taccttttga atagtacgtg gatgccgcat
     3361 tgaggaaaac cccatccata tccgatgcat ttttagtcat actgataacc ctgtcatact
     3421 gcttttcacc aaaaagcatc cattcagtag cacggtcatt cttcatggtt gattcttcgt
     3481 taacagtgtc ccattccagt tcaacgtttc cttcttctgc accacgtaca gcagcagttg
     3541 ttacaattcc agtagtatgt ttttctttac ggattttgtc ccatactgtt aggtttttgt
     3601 tttgttttgt tggagccaat gatacaacgg taatcttttg ttggggctgt atgctggtaa
     3661 caataccact cactgtatca aagatatgag cctgtgaacg tttgccggtg ataatgacag
     3721 cgttgggagg taaaaccatt ccaatttcag gttccacatt tttcccattg acctcaatct
     3781 taacatcgcc aataaaaaaa ttcaaacgtt gctctgcatg aagtaatgta aagggaaaaa
     3841 acaaaattaa aaaaaggaag aagaatctga ttttcattat taaagacccc cacgtaaata
     3901 tgaggggact ctaaaaactc tctgtatatg aggcgggtgc tacactatga taaaataaat
     3961 tgtttaatta aatgaatatt agttttaaaa atctcttaag ctataaaaac agcttctaaa
     4021 gccccctagc tatctggaat ataatatact ataaaaaatt agtcaagaaa taattattgt
     4081 ctatttaaaa gttttttctg ctatgagctg catggcgtca tcatacgcca ccatcgtctt
     4141 ccaccctagt tcagcttgcg tcaatgtagt atcaacatca ttgtccctgc ccatcactgc
     4201 cactgcaagc
//
LOCUS       DAORYL010000143         4161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_14355_length_4161_cov_13.609839, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000143 DAORYL010000000
VERSION     DAORYL010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14355_length_4161_cov_13.609839"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /gene="tpiA"
                     /locus_tag="PLV81_14850"
     CDS             complement(<1..743)
                     /gene="tpiA"
                     /locus_tag="PLV81_14850"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010047578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOM11417.1"
                     /translation="MNTKRKQIFAGNWKMYTTQKEAVNLALGIAEGLQKTDREIVIFP
                     PSVYVVCVKAACEGTPIKVGFQNMYFEKEGAFTGEIGPLMVKDTGASYVLIGHSERRH
                     IFGETNEMIHKKVVAAIAHGITPMLCVGELLEEREAGKSESTVETQIVKAFEGLTAED
                     ALKVVIAYEPVWAIGTGKVATPEIAQSMHAFIRKTLQTLYTDTVAETIPVLYGGSVKP
                     DNIAGLYAMPDIDGVLVGGASLKVQSFLD"
     gene            1616..1933
                     /locus_tag="PLV81_14855"
     CDS             1616..1933
                     /locus_tag="PLV81_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11418.1"
                     /translation="MLSHITMPTSHNVQQIFSTINVKTFMTHSLILIVLCAVLIAIQQ
                     SQQLYTVSMMWQFLQFFYGSTFAFEYTYSYCKPRHQCNIPLETFQPMITRHYRRYTIP
                     NAK"
     gene            1941..3080
                     /locus_tag="PLV81_14860"
     CDS             1941..3080
                     /locus_tag="PLV81_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11419.1"
                     /translation="MQYYNGNLYYVYKGCLYKDDRMIISSGKVNDAFASPNGIYYLSE
                     NRYIALHRSPLYCEVKGAHSIAYSHEHTWFAVYNEDFVAIIRPNCTLYKKYSFGKPIH
                     KAWIIFDDYYQIYVCAACDNYLYITDGIKTRRYYSVYSLTGGTEQTICHSNGFFIQGK
                     LITSNGFLSSQYSYPYDVCIDVTIGHPVDNAVSIQIPQAILSGHCFASIFSSDHCIYA
                     LVTKQLQNDVLSQGPIFTGSDNARITGKFYSYAFEIARDVDDWTIDAQYYSSVGNLYI
                     RQDQTITIIDIVKELENTITLSKDIAPHCCNTQPYAQCIEIKRSVLWQLSVLHSIGRF
                     LEKWIFTLIDEFPKIALKTILYIINYLFFLILFRLIIAIIKALLH"
     gene            3195..3434
                     /locus_tag="PLV81_14865"
     CDS             3195..3434
                     /locus_tag="PLV81_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11420.1"
                     /translation="MKTVKKLITLDQSLANDLELVAKSLNKTQREVIELALDFYFDYT
                     DGIIADKITDDIKKGTMKVYSSKEVYKKLGIDFEG"
     gene            3424..3696
                     /locus_tag="PLV81_14870"
     CDS             3424..3696
                     /locus_tag="PLV81_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOM11421.1"
                     /translation="MKVKYSEQAVKDLETFNAVDRQLIVKKIHYLAENFQQLKKSKKV
                     TQLKGTKYPDQYRYVIAKKIRAIFRVENNELILLVLRIGLRKNIYE"
     gene            complement(3760..>4161)
                     /locus_tag="PLV81_14875"
     CDS             complement(3760..>4161)
                     /locus_tag="PLV81_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOM11422.1"
                     /translation="AAALSMIPTTTGAAKAVALVLPELKGKLDGLAIRVPSPDVSVVD
                     FVCTVKTSTTAEDVNKALREAANGKMKGILQVCDEELVSIDFKGNTHSSIVDAPSTAV
                     MGGNMIKILSWYDNEWGYSCRVVDLVQYCMK"
BASE COUNT         1325 a          832 c          701 g         1303 t
ORIGIN      
        1 atgtcaagaa aagattgtac tttaaggctt gcaccaccaa ccagcacacc gtctatgtca
       61 ggcattgcgt acaaccctgc aatattatcc ggtttaacag aaccaccata caacaccggg
      121 atagtctctg ccacggtatc agtatacagc gtttgcaatg tttttctaat aaaagcatgc
      181 atggattgtg ctatttcagg tgttgcaact ttgcctgtgc caattgccca cacaggctca
      241 taggcaataa ccactttcag cgcatcttct gcagttaatc cttcaaaggc ttttactatc
      301 tgcgtttcaa cagttgattc ggattttccc gcttcacgtt cttctaaaag ttcacctaca
      361 cagagcatgg gcgttattcc atgagctatc gccgccacta cttttttgtg tatcatctca
      421 ttggtttcac caaagatatg cctgcgttcc gagtgaccaa taagaacgta agaagctcca
      481 gtatccttaa ccattaaggg cccaatctcc cccgtaaagg caccttcctt ttcaaaatac
      541 atattctgga aaccaacctt aataggcgtt ccttcacagg cagccttgac acacactaca
      601 tacaccgatg gaggaaaaat cactatctcc ctgtcagttt tttgaaggcc ttcagcaata
      661 cccagagcaa ggtttactgc ttccttctga gtggtataca tcttccagtt tccagcaaat
      721 atctgttttc gttttgtatt cataatgttc cctttttgtg ttatttttcc atgcaataat
      781 cctcaatcta taaaccacag atactatatt ttttcacaaa tgcaacgatt ttacaaaatt
      841 ttttaaaggg acattatatg tcattatcaa tgattatcat acctatgaaa aggttgcaaa
      901 gaaattttta taatacatgt aacatagtaa tcgtcatata cttggggcga tagttcctga
      961 taacagtcgc aggtgtttaa tcttacttca ggctgaaaac agaacgcgac cgtttaatct
     1021 ttgtaaggag aaaacttatt ttaaccccga tgcaatcggg gattcgaaat gacatgtcgg
     1081 aatagatgaa taccacaata tttgtacact attattttga taacgaatgc tcttaccttg
     1141 tcatttcgac aacgaatgtt tttaccttgt catttcgacg aacgcagtga ggagaaatct
     1201 tatgttattt ctttgtcatt ctttagtgct atcacacctt tagaattaac ttgagtggca
     1261 aaaagatttc tcatcccttc ggggattcga aatgacatgt tgtaatagat gaagaacaca
     1321 atatttgtac actgttattt tgacaacgaa tgctcttaca ttgttattcc gacaacgaat
     1381 gtttttacct tgtcatttcg acaacgaatt tttttacatt gtcatttcga cgaacgcagt
     1441 gaggagaaat cttatgttat ttctttgtca ttctttagtg ctatcacacc tttaaaatga
     1501 ccttgagtgg caaaaagatt tctcatccct tcggggattc gaaatgacat gttaggattt
     1561 ctcatcccga tacaatcggg aattcgatac atcattatac tgtttggagg tacatatgct
     1621 ttcacatata acaatgccta catcacacaa tgttcaacaa atattctcca ccataaatgt
     1681 aaaaaccttt atgacacatt cactgatcct tattgtctta tgtgcagtat taattgcaat
     1741 ccaacagtcc cagcaactgt atacagtaag catgatgtgg caattcttac agttttttta
     1801 tggatcaaca tttgcttttg aatatacata ttcctattgt aaacccagac atcagtgcaa
     1861 catcccgctt gaaactttcc agcctatgat aacacgccat tacaggcgct ataccatacc
     1921 caacgctaaa taggatagcc atgcaatact ataatggaaa tctgtattat gtgtataaag
     1981 gctgcctgta taaagatgac cggatgatta tcagttcagg gaaggtaaac gatgcctttg
     2041 caagtcccaa tggcatatac tatctgtccg aaaacagata tattgcgttg cacagatcac
     2101 cattatactg cgaagttaaa ggagcacatt ctattgcata cagccatgaa catacctggt
     2161 ttgcagtgta taacgaggat tttgttgcca ttataaggcc caactgcaca ttgtataaaa
     2221 aatattcatt tggtaaaccc atacacaagg catggattat atttgatgac tattatcaaa
     2281 tctatgtttg tgccgcctgt gataattatc tatacataac cgatggaata aaaacgcgca
     2341 gatattatag tgtatattca ctaacagggg gcaccgaaca aactatttgc catagtaatg
     2401 gattttttat acaaggcaaa ttgataacaa gcaatggttt tctttcttct cagtattcat
     2461 atccatacga cgtatgtatt gatgtcacca tcggtcatcc tgtagataat gcagtcagca
     2521 ttcaaatacc ccaagccata ctatcaggcc attgctttgc ctctatcttt tcaagtgacc
     2581 actgcatcta tgcacttgta acaaaacaat tacaaaatga cgtgttgtct cagggaccaa
     2641 tttttactgg cagtgacaat gcccgtatta ccgggaaatt ttactcatac gcatttgaaa
     2701 tagctcgtga tgttgatgac tggactattg atgcacagta ttacagtagc gtcggtaatc
     2761 tttatattcg tcaggaccag actataacta taatagacat tgtaaaagag ttggaaaaca
     2821 caattacgct ttctaaggat atagcaccac attgttgcaa cactcaacca tatgctcagt
     2881 gcattgaaat taaaagatct gtgttatggc aactttcggt attacattct attggccggt
     2941 ttcttgaaaa atggatattc acacttatag atgaatttcc aaaaatagca ttaaagacaa
     3001 tactatatat tataaattat ttatttttct tgatactatt caggttaatt attgcaataa
     3061 ttaaagcttt gttgcattaa acagactaaa acattgaatt gcattttgtt acagagaaac
     3121 tcatcattta aaaatgcttg acaatatata taaatagata tatatttata tatactatta
     3181 ttaacaggta aaaaatgaaa acagtaaaaa aactcataac attagatcag tcccttgcca
     3241 atgacctgga actggtggct aagtctttaa acaaaacgca gcgtgaagta attgagctgg
     3301 cgcttgattt ttactttgat tatactgatg gaataattgc ggacaaaata actgatgaca
     3361 taaaaaaagg cactatgaaa gtgtacagca gtaaagaagt ctataagaaa ttaggaatag
     3421 attttgaagg ttaaatactc tgagcaagca gtcaaggatc ttgaaacatt taacgctgtt
     3481 gatcgtcaat taatagtaaa aaaaatacat taccttgctg aaaattttca gcaattaaaa
     3541 aaatcaaaga aagtaacaca gttgaaagga acaaaatacc ctgatcaata tcgttatgta
     3601 attgcaaaaa aaatacgagc tattttcaga gttgaaaata atgaattaat acttcttgtt
     3661 ttacgtattg gattgcgaaa aaatatatat gaataatgtt atctatttac ctatatcccc
     3721 gccgccagag gcagcgagga tatgtcctat tacgtgttac tatttcatgc aatactgaac
     3781 taaatcaaca acacggcagg agtagcccca ctcattatca taccagctca atatctttat
     3841 catgttgcct cccatgaccg ccgttgatgg agcatcaacg atagaagaat gggtattgcc
     3901 tttgaaatct attgacacca attcttcatc gcaaacctgc aaaatgcctt tcattttacc
     3961 atttgctgct tctcgcagtg ctttgttgac atcctcagcg gtggtactgg ttttaactgt
     4021 acatacaaaa tccactaccg aaacatccgg tgaagggacg cggattgcca gcccatctaa
     4081 tttgcctttt aattcaggca gtaccagcgc aactgccttt gctgcacctg ttgttgtggg
     4141 tatcatagaa agtgcggcgg c
//
LOCUS       DAORYL010000144         4142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_14428_length_4142_cov_12.375092, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000144 DAORYL010000000
VERSION     DAORYL010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14428_length_4142_cov_12.375092"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(564..1040)
                     /locus_tag="PLV81_14880"
     CDS             complement(564..1040)
                     /locus_tag="PLV81_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11423.1"
                     /translation="MNAETTTCISLTHDKILKEICKKYNLSITTIVVLMVKFAVERQK
                     LSIISNRNVKYRNKNCSIWRRIHLQLSPNDYEFLMDVKKIWKISVAKMIEYCIDNVLF
                     ELQEKVLEKNKTDNYLYNNYHFEIGEEEGLTYYLIYWGLPLKLLKKLTLQNSILCP"
     gene            1449..4103
                     /locus_tag="PLV81_14885"
     CDS             1449..4103
                     /locus_tag="PLV81_14885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOM11424.1"
                     /translation="MDFKSTFNEQIVSMLNIFVNEQKKRFPSNEVLTIDLHCHDHNSN
                     IPDEQLGRILGIPETWLPTEDLLTTLKNHGCDTYTVTNHNNARSCYELLDKGHDVLVG
                     AEFSCTVPDYKVGIHVLAYGFTPEKEIKLNKLRTDIYKFLDYTLENDIPTIWAHPLYH
                     YKSKGIPPMEFFDKMALLFERFEVINGQRDSWQNMLVKQWIESLTQDKLEELSHKFKI
                     KPDRYCTTPYTKYMSGGSDSHMGIFTGLTGTYLHIPDLAERLKTEKRSALALEAIKNG
                     AMAPFGTHNDSEKMAISFIDYFCQIGMNMKDPGLPHILLHKGDVQDKLSALAITNAFH
                     ELKRHKTTLNFLELFHKSLIGKTFSKTKRLYVSKDYKKVFDIAYKLSRYTKTRKKDDV
                     TFYTQSVYGIYKELVQLLMKRSDKHINELINNSRFKSKEFESFFENIEIPTHFRKYIE
                     GNEKPNGNGSARVNLPKLFDELSFPFLGSAVIATAFFTSARVMYKARPLLYEFAKAHN
                     TLKHPERTLWLTDTFTDSNGVALVLQSMLKEIQTRDLPIDIITISSTLQPQDHLIVIR
                     PLKEFTLPFYEQQPLRIPDILEIHNIFKQGEYSKIIASTEGPMGLAALWLKYAYSVPA
                     YFYVHTDWMMFAEQTLKLTDENLHNFRRLLRTFYKGFDGLFVLNNDHRKWLTGKDMGF
                     NPSQVHLTAHWVEETFKPYKINKEDIFGVSNTTPVVLFAGRLSEEKGVMDIPKVMEIV
                     KQKHPDAKVAFVGIGPKEQELRKLLPDAIFLGWVQHDTLPKIYSAADVLVLPSRFDTF
                     GCVVLEALSCGLPVLAYNTKGPKDIINNGLNGYLVKNEIEMGQKITELLDNPLLLNLF
                     KNQSLLRAHEFSPDAIIKQFINDLNSAA"
BASE COUNT         1448 a          741 c          642 g         1311 t
ORIGIN      
        1 acataattta gtcaattaat ttcttgactt tttttgagaa aatccccacc ctcatatata
       61 taagaataac aaagctgact gctgctatta ttgttaataa taatccagta ttcatgattt
      121 tctggtttct acttttataa tacttttacg tttttttcgg aatgccaaag aactcagtac
      181 cataccggat accaatccaa ttattaaagt agcaaaaagc attacaataa atggcagagt
      241 gatattccac gcaagtaaag ttaatgaaac cggttgggta ttctgtaata ttattataag
      301 aaacaaaaaa agaataattg aaatgattat tacttttact cgcatacatc taccctgttg
      361 tattgactaa acatattttg atgcatagaa cattatacta tagcatttat aagttttgta
      421 taattaagca atagtctctg tctctcccaa taaataaaaa gataatacga gaatatttgt
      481 tgcaactggt aaaatttaat tatattgata tagcaatagt aaaatatgtc aagtaaagta
      541 tagtattgat gtgtatatta ttactatggg caaagtattg agttttgcaa ggtgagtttt
      601 ttcagtaatt ttagcggtaa tccccagtaa atgaggtaat atgttagtcc ttcttcttca
      661 ccaatttcaa aatgatagtt gttgtataga tagttatcgg tcttattttt ttctaaaact
      721 ttttcctgca attcaaaaag tacattatct atacaatatt caatcatctt agcaacagat
      781 atcttccata ttttcttaac atccattaaa aattcataat catttggact taattgcaaa
      841 tgaattcttc tccagatgga acaattttta tttctatact ttacgtttct gttgctgatt
      901 attgataatt tctgtctttc aactgcaaat ttaaccatta atacaacaat tgttgtgata
      961 gaaagattat actttttgca aatttccttt aaaattttgt catgagtaag agaaatacag
     1021 gttgttgttt cagcattcat aaagtactcc atttatgttt atttatgtta ctattgcttt
     1081 tttaaacaaa attaaattta actttttcaa caacttctac tttggtatgt tgacattaat
     1141 atttaatatt ttttgcatta attatatgat ttattgtcga tatttacccg caaatatcac
     1201 aaccacattt taacatttcc ttcccacagt gtcatcaacg catagattaa acaattttaa
     1261 taaggatatc atcttttaaa gtttttattt tttattactt tatatactca cccatttaac
     1321 aatattaaaa cctaatataa ataatcaaga ttttttcacc acaactaaac ataaacttca
     1381 taaaatcttt acacatccac gtcatattat catcgagatt taaggatata gaaactttaa
     1441 gaggagatat ggattttaaa agtacattta atgaacaaat tgtttcaatg ctaaacattt
     1501 ttgtaaacga acaaaagaaa cgctttcctt caaatgaagt gttaacaatt gaccttcact
     1561 gccatgacca caacagcaat atccctgatg aacagttagg aagaatatta ggaatccccg
     1621 aaacatggct accaaccgaa gatttactta caactctgaa gaatcatggt tgtgatactt
     1681 atacagtaac aaatcataac aatgcccgat catgttacga gcttcttgat aaaggacatg
     1741 atgtacttgt tggcgctgaa tttagctgca cagtacctga ttacaaagta ggtattcatg
     1801 tgcttgcata tggctttact cctgagaaag agataaaact taataaactc cgtactgata
     1861 tatacaaatt cttagattat actcttgaga atgatatccc aactatctgg gcacacccat
     1921 tgtatcacta caaatcaaag ggcataccac ccatggaatt ttttgataag atggcccttt
     1981 tatttgaacg ctttgaagtt atcaatggac agcgcgatag ctggcagaat atgctggtta
     2041 agcagtggat tgaatcacta acacaggata agttagaaga gttatcgcac aaattcaaaa
     2101 taaaaccaga tagatattgt acaacaccct acactaagta tatgtcaggg ggcagcgata
     2161 gtcacatggg aatcttcact ggcttaacgg ggacatatct gcatatccca gatttagctg
     2221 aaagattaaa gaccgaaaaa cgttcagcac tggcacttga agccataaaa aatggtgcaa
     2281 tggcaccttt tggtacacat aatgacagtg aaaagatggc aatttctttc attgattact
     2341 tttgccagat aggcatgaat atgaaagacc ctggccttcc ccatattctg ctacacaaag
     2401 gcgacgttca ggacaagcta tctgcactgg ccataaccaa tgctttccat gaattaaaac
     2461 ggcataaaac cacgttaaac tttttagagc ttttccacaa atcattaatt ggaaaaacat
     2521 tttccaaaac aaaacgattg tatgtaagta aagattataa aaaggttttt gatattgcct
     2581 acaaattatc acgatataca aaaaccagaa aaaaagatga tgtgacattc tatacacaat
     2641 cagtatatgg aatatataaa gagcttgttc aattgctcat gaaacgttct gataaacata
     2701 taaacgaatt aattaataac agcagattta aatcaaaaga gtttgaatca ttctttgaga
     2761 atattgaaat ccctacccac tttagaaaat atattgaagg caatgaaaaa cctaatggta
     2821 atggttcagc cagagttaac ctgccaaaac tttttgacga gttgtccttc ccatttttgg
     2881 gctcagcggt cattgcaaca gcctttttta ccagtgcccg agtaatgtat aaagcacgac
     2941 cactacttta tgaatttgca aaagcacaca atacgcttaa acaccccgaa cgcacactat
     3001 ggcttactga tactttcacc gattccaatg gtgttgctct tgttctgcaa tctatgctta
     3061 aagaaattca aacacgagac ctacccattg atattattac tattagcagc acacttcaac
     3121 cacaggatca cttaattgtt attcgcccat taaaagaatt tacccttcca ttctatgaac
     3181 aacaaccttt gcgaatccct gatattctgg aaatccataa catcttcaag caaggtgaat
     3241 acagtaaaat tattgcttca acggaaggcc caatgggatt agctgcactg tggttaaaat
     3301 atgcatattc tgttcctgca tacttctacg tgcatactga ctggatgatg tttgcagaac
     3361 aaacattaaa gctaaccgat gaaaacttac ataatttcag acgtttattg cgcacatttt
     3421 ataaaggatt tgatggactg tttgttctta ataacgacca ccgcaaatgg ttgacaggta
     3481 aagacatggg ttttaatcct tcacaggtac acttaactgc acactgggtt gaagaaacct
     3541 ttaagccata taaaataaac aaagaagaca tttttggagt aagcaacaca acacctgttg
     3601 tactttttgc aggaagactt tcagaagaaa aaggtgtcat ggatatacca aaagtcatgg
     3661 agatagttaa gcaaaaacat cctgatgcaa aagttgcatt tgttggcatt gggccaaaag
     3721 aacaagaact aagaaagctt ttgccagatg caatcttttt gggatgggtt cagcatgata
     3781 ccttaccaaa gatatactca gcagcagatg ttctagtatt gccatcacga tttgatacat
     3841 ttggatgtgt agtgcttgaa gcgttatcat gcggactgcc tgtacttgct tacaacacaa
     3901 aaggacccaa agatatcatc aacaacggat taaatggata tctggtgaaa aatgaaattg
     3961 aaatgggcca aaagatcaca gaacttctgg ataatcctct gcttctgaac ctcttcaaaa
     4021 atcaatcatt gctccgtgct catgaattta gccctgatgc aatcatcaag caatttatca
     4081 atgaccttaa cagcgcagca taatcaacaa aagcatatat ggtggaagca cggggtaata
     4141 ta
//
LOCUS       DAORYL010000145         4140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_14439_length_4140_cov_12.928274, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000145 DAORYL010000000
VERSION     DAORYL010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14439_length_4140_cov_12.928274"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PLV81_14890"
     CDS             <1..779
                     /locus_tag="PLV81_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11425.1"
                     /translation="YVENNIQYINEIHKKIEKLILKLEKEHISIKECFTQLENEINLL
                     LKVYSHFTSLGGYNYCLKLKALIDSYQKVTNTEIQLSALYYIHSKILELQEEQLKHFP
                     ELTQKKALYDKLTSTKKFVEDDVHNCTYKWITFKRNAMWFVVLFDSYQLLNTSDVSGL
                     KPLSQNYYQATFNNTTITIRDPLRLDEQPSTTVLLVNYRNSIYGYFYDSIGKKLGARS
                     NILQQMAKAPEGYSLPLYGRVRLFGDSYIYLKHVDLNKPQ"
     gene            complement(780..1448)
                     /locus_tag="PLV81_14895"
     CDS             complement(780..1448)
                     /locus_tag="PLV81_14895"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HOM11426.1"
                     /translation="MMQFVESPYKNDDVIIIRKLKKDGSVDIEIIQKITQILHKNGLV
                     ILPVDSQYEIVGIANAAVEHKLKTIIKSKTKKFVRLIASFKMLESLATVTKIQYDFLH
                     RIWPGEVTAIVPRKEDVSQEIALRYPKTRFVQEIIETVGQPLFATNILRVTKGSNKHS
                     DIVRVFGKKVDAIVIIEELCKRHPKPTLISLFNGKLKILRPGKISSEEIQSYYYLGSC
                     DEEV"
     gene            complement(1414..1959)
                     /locus_tag="PLV81_14900"
     CDS             complement(1414..1959)
                     /locus_tag="PLV81_14900"
                     /inference="COORDINATES: protein motif:HMM:NF016314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HOM11427.1"
                     /translation="MYTLTPRDEQVLRWQLKGTTDAVAGIFEKCDYGFPQIVLLSPRA
                     GGAVDYQSLSNILWLTCPYLNDKIHELENKGYIAKIETFINTDEVLKREMLKAHSHFY
                     FYRKSVYEKYVSKEIEDEYLQVMKRGIGGLQEPVHLKCLHLHYAHYRICENNIAGRIV
                     YHLLQQKVNCDDAICRVSLQK"
     gene            complement(1988..2440)
                     /locus_tag="PLV81_14905"
     CDS             complement(1988..2440)
                     /locus_tag="PLV81_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11428.1"
                     /translation="MAKRCFIQLASRNNKFSIFLIAFILFSVYCFVFSESGILERMQL
                     QKEIADIQLQIIQKQSEIKKFRELTNKGNPNNLLLKESINAGYIPQGAKVFEFKDKPK
                     EPPQIQTSQPLITEKFTQYIQYGRILWVIFSVLIITGMLLYYRNRNQL"
     gene            complement(2403..3695)
                     /gene="eno"
                     /locus_tag="PLV81_14910"
     CDS             complement(2403..3695)
                     /gene="eno"
                     /locus_tag="PLV81_14910"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOM11429.1"
                     /translation="MTIITDVHAREILDSRGNPTIEVDVELSSGIVGRFAVPSGASTG
                     ENEAIELRDGDKSRYNGKGVLKAVQNVNEIISGAVVEMDAMRQIEIDKTLIDLDGTPN
                     KAKLGANAILGVSMACAKAVAETFGMPLYQYIGGVGACELPVPMMNILNGGKHADNNV
                     DIQEFMIVPVGADSFKEALRMGVETFHALKKVLSKKGYNTAVGDEGGFAPNLKSNAEA
                     FEVILEAIKEAGFKAGQDIYLAIDSAASEFYKNGKYVMAAEAKPEKTSEEMVQVYIDW
                     INKYPIISIEDGMAEDDWDGWKLLTDKCKDKVQLVGDDIYVTNTEIFKKGIEKGIANS
                     ILIKLNQIGTLTETIEAIEMAKRAGYTAVVSHRSGETEDPSIADLAVAMNTGFIKTGS
                     ASRTDRLSKYNQLLRIEEQLGAAAKFNGKKVFYSIGKP"
     gene            complement(4030..>4140)
                     /locus_tag="PLV81_14915"
     CDS             complement(4030..>4140)
                     /locus_tag="PLV81_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOM11430.1"
                     /translation="NYYEHIVRNQMELKRIREYIVNNPVNWQNDENYIKT"
BASE COUNT         1224 a          840 c          654 g         1422 t
ORIGIN      
        1 catacgtaga aaacaatatc caatatatta acgaaatcca caagaagatt gaaaagctta
       61 ttttaaaact tgaaaaggaa cacatctcaa ttaaggaatg ttttacccaa cttgaaaacg
      121 aaattaatct gctattgaaa gtgtattcgc atttcacatc gttgggtgga tacaactact
      181 gccttaaatt aaaagccctc attgactcat atcaaaaagt taccaatact gagattcagc
      241 tatctgcgct gtactacatt cattccaaga ttcttgaact acaggaagag caattgaaac
      301 atttccctga gttgactcag aagaaagctt tatatgataa gcttacttcc acaaaaaaat
      361 ttgtggaaga tgatgtacac aattgcactt acaaatggat taccttcaaa cgtaatgcca
      421 tgtggtttgt tgttcttttt gatagttatc agttgctaaa taccagtgat gtatcaggtc
      481 ttaagcctct ttcacaaaat tactatcagg caacatttaa taacaccaca attacaatcc
      541 gtgatcctct gcgattagac gaacagcctt caaccactgt attgcttgtc aattaccgca
      601 acagcatata tggttacttt tatgattcaa ttgggaaaaa attaggtgcc cgtagcaaca
      661 ttctgcagca aatggcaaaa gcgcccgagg gctattcatt gccattatac ggaagggtca
      721 ggttatttgg cgatagttat atttatttaa aacatgttga tttaaataag ccgcagtaat
      781 tatacttcct cgtcacagct gcctaaataa tagtatgact gaatttcctc ggaagagatt
      841 ttccctggcc gcaatatctt gagtttacca ttaaagaggc ttatgagtgt tggcttaggg
      901 tgacgtttgc acaactcttc gataataaca atggcatcaa ctttttttcc aaatacacgt
      961 actatatcag aatgcttatt tgagcctttt gttaccctaa gaatatttgt ggcaaataga
     1021 ggctgtccca ctgtttcaat tatttcctga acaaaccgtg ttttgggata acgcaatgca
     1081 atttcctgcg atacatcttc ctttcgtggc acaattgcag taacctcgcc aggccatatc
     1141 ctatgcagaa aatcatattg aattttagta accgttgcaa gggattctaa cattttgaaa
     1201 gatgctataa ggcgcacaaa tttttttgtt ttggatttaa tgattgtttt taatttatgc
     1261 tcaaccgctg cattggcaat gcctactatc tcatattgag aatcaacagg aaggataaca
     1321 aggccatttt tatgtaaaat ttgggtaatt ttttgtataa tttcaatatc tacactacca
     1381 tcttttttta gctttcgtat tattattaca tcatcatttt tgtaaggaga ctctacaaat
     1441 tgcatcatcg caatttacct tttgctgtaa taaatgatac acaatccttc cagctatatt
     1501 gttttcacat attctataat gtgcataatg caaatgcaag cattttaaat gtacaggttc
     1561 ctgcaaacct cctatgcccc ttttcatgac ttgtaagtat tcatcttcaa tttctttact
     1621 tacatatttt tcatatacac tttttctgta aaaataaaaa tgagaatgtg ctttcagcat
     1681 ctcgcgcttt aacacttcat cagtattaat aaatgtttca atttttgcaa tatatccttt
     1741 attttcaagc tcatgtattt tatcattcaa gtatgggcac gttagccata gtatattgga
     1801 caatgactga taatcaaccg cgccaccagc ccttggggat aacaatacta tctggggaaa
     1861 tccataatca cacttttcaa atattccagc aacagcatca gtggtaccct taagctgcca
     1921 gcgtaaaacc tgctcatcac ggggtgtaag tgtgtacatg tgcggtatca tacattgaat
     1981 aagcaattta taattgattt ctgtttctat aatatagcaa cattcctgta ataatcaaca
     2041 cagaaaatat cacccataaa attctgccat actgtatata ctgagtaaat ttttctgtga
     2101 ttagcggctg tgaggtttgt atctgagggg gctcttttgg tttatcttta aactcaaata
     2161 cttttgcacc ctgagggata taccctgcat taatgctttc cttcaataac agattgttag
     2221 gattaccttt gttggtcaat tccctgaact ttttaatttc cgattgcttc tgaataatct
     2281 gaagctgtat atctgctatc tctttttgca gctgcatcct ctccagtatg ccactttcac
     2341 tgaacacaaa gcaatacaca ctgaagagga tgaatgcaat cagaaatatt gaaaatttat
     2401 tattacggct tgccaattga ataaaacacc tttttgccat taaattttgc tgcagcaccc
     2461 agctgctctt caattcgcaa taactgattg tattttgaaa ggcggtcagt acgcgatgct
     2521 gatcctgtct tgataaaccc tgtattcata gcaacagcta aatcagcaat tgatgggtct
     2581 tctgtttcac ctgaacggtg ggacacaact gctgtatacc ctgcccgttt tgccatttca
     2641 atagcttcga tagtttcggt caatgtgcct atctgattca atttaattaa gattgaattg
     2701 gctatcccct tttctatgcc ttttttaaat atttctgtat tagtaacata tatatcgtcg
     2761 ccaacaagct gcactttatc cttgcattta tcagtcaaca gcttccatcc atcccagtca
     2821 tcctcggcca taccatcttc aattgaaatg attgggtatt tgtttatcca atcaatatat
     2881 acctgaacca tttcttcaga agtcttttca ggttttgctt cagctgccat aacatacttg
     2941 ccatttttgt aaaattcact tgccgctgaa tctattgcca gataaatatc ctgacctgct
     3001 ttaaatccag cttctttaat tgcttccaat attacctcaa aagcttcagc attggatttt
     3061 aaatttggtg caaatccacc ttcatcgccc actgctgtgt tataaccttt tttggataac
     3121 acctttttta atgcatggaa cgtttcaact cccatgcgca acgcttcttt aaatgaatca
     3181 gcgccaaccg gcacaatcat aaattcctga atatccacat tattgtcagc atgtttcccg
     3241 ccattcaata tgttcatcat tggcacaggc aactcacatg cacccactcc accaatatac
     3301 tgatacagag gcataccaaa ggtttcggca acagccttgg cacatgccat tgatacgcct
     3361 aaaatggcat ttgcacctaa ttttgcttta tttggtgtac catctaagtc aatcagcgtt
     3421 ttatctattt ctatctgtcg catggcatcc atttcaacaa cagcgcctga tattatttcg
     3481 tttacgtttt gcactgcctt caatacacct ttgccattgt agcgggattt atcgccatcc
     3541 ctcagttcaa tggcttcgtt ttcgccagtg gatgcacccg atggtacggc aaatctgcca
     3601 acaatccctg aagatagttc aacatcaact tctatggtag gattgccccg tgaatctaaa
     3661 atttcacgtg catggacatc tgttataatt gtcatatcag tactcctgta taataaattt
     3721 ttagctgagg cattattttg tgattacatt acaatgtgat tacaaatagc aaatatatgc
     3781 aagaagtttt ttgtggtggg ggtgtttgat ggcattttgt tgcaatcatg gataatggta
     3841 attttaaatc aacacatgat tgtttattat tttctccttc ctcatttaat acgatgtttt
     3901 caccaaccac attgccaaac aaacattcgc ggttttgtgt acagatggtg atgtaatata
     3961 cattcggctg gtaaataatt gtaatttttt aataataata atatggatag aattgcaata
     4021 atttgatttt tatgttttta tataattttc atcattttgc caattaacag gattattaac
     4081 aatgtattcc ctgattcgtt ttaattccat ttgattgcga acaatatgtt cataataatt
//
LOCUS       DAORYL010000146         4097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_14622_length_4097_cov_6.082385, whole genome shotgun sequence.
ACCESSION   DAORYL010000146 DAORYL010000000
VERSION     DAORYL010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14622_length_4097_cov_6.082385"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>4097
                     /locus_tag="PLV81_14920"
     CDS             <1..>4097
                     /locus_tag="PLV81_14920"
                     /inference="COORDINATES: protein motif:HMM:NF015497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HOM11431.1"
                     /translation="SNCWTVTHKNGVRYIYGETQNARLQQGNKTGQWYLEWMIDPNGN
                     SVHYTYKRLTGILPGSSTSYVQVYLDTIYYTGYNKTNGPFAITFTLDDGTRPDKQIDG
                     RMGFIVYSAYRYKEITVSYGGQVVWRYVLNYTTGAFDKSLLQRVEVRSGSGQLFYTHD
                     FDYYDEIGWGNEHLNLFDSEVVIGYTANVSEKLYGVNYYNFGNIGGSKSTTNQFQFSG
                     NFAPGNPMKMFSVGAEYSISKTEMSTKIILIDIDGDGLSDQVYSDNGLKYKRNLMGEG
                     ISGFADEKEINGLPSLGDDINISKTKGVGLNTGMAGERFQKGNSESRTTSYFTDVNGD
                     GLVDFIKDGIVYYNHGYNDTLQVVQFSTTIPEGTEPVTGEVVAPVSPVDDGEETESKY
                     AFLEYYFRDNPVLAWRAPFDGKITISGNVTLNKSVPNDYATADGVRVSIQKTGIQHSA
                     LPKVEEITRGSSKEMTLDNIEVKSGEYIIFRVDSNRDGAYDEVQWNPIITYKGYVPEA
                     VDENNLYIYRYTLANDYYVYGAGEFEAPITGVVTLYGGKIFKYSKTSDDILVRITKKR
                     ITQTFDCVNNTIIDSNVETLWEYILPVKADFIGTEYIPDRDDEEVENRETRVNGENNT
                     VQSITSIKTIFECTLQSDTPIDYRQVQWENTDGYKGPLLRYKDINSGSGDNQLSEESI
                     NKIKERIFNIPVVTSLFTIPTVYIENERQIPMDPYIFEGWGVPREGTYRITMTATNTS
                     SIPYTGSLALSIKKRRHGNGFIADVAEKVVNDVTVGILPGETLQLQRELTLDESDDMY
                     FIIATAKTAGLQLNVHVEYNVFSNNPWVNGDEMWVDIPGVAYIVYTAVDPSNDISFAG
                     GFRNWYYGRWNADSYMYGNDAPDTYDYEFEPIQTDLMKINVDEASNNPQNFAKKLTIC
                     SSMIPKYKEKTDDELQEEISKENPHTTDPSFPVWRGMDDDCWIGPGVFATTRVAKKNI
                     EDLLHASDTQVIETELLQTRALNSGNTGVVDKRGITKITKTYNDTVSFSGRGSSVTVT
                     TSTGYTKIQFFDVNGDRYPDILTDNGLVFTGINGLYAGMTGAFSGSVQKFVSTNYNLG
                     ICPQSSASQVETIPQGDGYIVKVKTSFSNLSFSLATGDNNIIVDYQDINGDGLPDRIE
                     YNGFAGYSVRYNTGYGLSPKCAFKNLYGIQHTKNKSRSLSVGFSTDKSIISGGVSVVE
                     NSSEKLDVPLLNEGDRYTLIDINGDGLPDRIVEYDHGIYIFVNTGEGFVLYAKKSDGK
                     MDETKGMSCTPSVTLGVKFTILPLVTIGTIIIDGGVSLTKDGNSTRYALRDINGDGLP
                     DIIYDDNGTLKAKLNRTGKTNMLKSIKNSIGGTIELDYKRVGNTRL"
BASE COUNT         1441 a          575 c          926 g         1155 t
ORIGIN      
        1 agtaattgct ggacagtgac acacaaaaat ggggtgcggt atatatatgg tgaaacacag
       61 aacgcgcgat tgcaacaggg aaacaaaaca ggacaatggt atttagaatg gatgattgat
      121 cccaatggaa atagtgtaca ctatacatat aaacgattaa ccgggatatt accgggatca
      181 tcaacatcat atgtgcaggt atacctggat actatatact ataccggata caataagacg
      241 aacggtccat ttgcaataac ttttacgctg gatgatggga cacgaccgga taaacagata
      301 gacggcagaa tgggctttat agtgtattca gcataccggt ataaagaaat aacggtgagc
      361 tatggtgggc aagtagtgtg gcggtatgtt ttgaattata caacgggagc atttgataag
      421 agtttactcc agcgggtgga agtacgttca ggcagtggcc agcttttcta tacgcatgat
      481 tttgattact atgatgagat aggctggggt aatgagcatt taaacctgtt tgattcagag
      541 gtagtaatag gatatacggc aaatgttagt gagaaattat atggagtgaa ttattataat
      601 tttggtaata ttggtgggtc aaaatcaaca accaatcaat ttcagttctc tggtaatttt
      661 gcaccgggta atcccatgaa gatgttttcg gtaggggcag aatatagcat atcaaagaca
      721 gagatgtcta caaaaataat tttaattgat attgatgggg atgggttgtc agatcaggta
      781 tattcagata atgggttgaa atataaacgt aatttaatgg gtgaaggcat tagtggtttt
      841 gctgatgaga aagagattaa tggtttacca tcgttagggg atgatataaa tatatctaaa
      901 accaaagggg taggacttaa tacaggaatg gccggcgaac gttttcagaa gggaaattct
      961 gaatccagaa caacaagtta ttttaccgat gtaaatggag atggattggt tgattttata
     1021 aaagatggta tagtttacta caatcacggt tacaatgata cattacaggt ggtgcaattt
     1081 tcaactacta ttccggaagg aacagaacca gtaactggtg aagtagttgc accagtatca
     1141 ccagttgatg atggggagga aactgaaagt aaatatgcat ttttagaata ctatttcagg
     1201 gataatcctg tattggcgtg gcgtgcgccg tttgatggta agataactat cagtggtaat
     1261 gttacgttaa ataaaagtgt acctaatgat tatgcaacgg ccgatggtgt aagggtaagt
     1321 attcagaaaa ccggtataca acattcagca ctaccaaaag tagaagaaat aacacgagga
     1381 agcagcaaag aaatgacact ggataatata gaggtaaaaa gcggagaata catcattttt
     1441 agagttgatt ccaatcgtga tggagcgtat gatgaagtac aatggaatcc aataataaca
     1501 tataaaggat atgtgcctga agcagtggat gagaacaatc tctatatata ccggtacaca
     1561 cttgcaaatg actattatgt atatggagcg ggagagtttg aggcaccaat aaccggggtt
     1621 gtgacattgt atggaggaaa gattttcaaa tattcaaaaa cgtcggatga tatactggtc
     1681 agaataacta aaaagcgtat aacacaaaca tttgattgtg ttaataatac aattattgat
     1741 tcgaatgtag aaacattatg ggaatatata ttgccagtaa aagctgattt tattggaacg
     1801 gaatatatac cggacagaga tgatgaagaa gtagaaaata gagaaacgcg agtaaacggt
     1861 gaaaacaata ctgtacaatc aataacatcg attaaaacaa tttttgaatg tacattgcaa
     1921 agcgatactc caatagatta taggcaggtt caatgggaaa atactgatgg atataaagga
     1981 ccattattac ggtataagga tatcaattca ggttcgggtg ataatcaatt atcagaagaa
     2041 agtatcaata aaattaaaga aagaatattt aatattccgg ttgtaaccag cctctttacc
     2101 ataccaacag tttatattga aaatgaaaga caaattccca tggatcctta tatatttgaa
     2161 gggtggggag ttccccgtga aggtacatat cgtattacaa tgacagcaac caatacaagc
     2221 agtattccct atacaggcag tctggcgtta agtataaaga aaaggaggca tggtaacggt
     2281 tttatagctg atgtagctga aaaggtagta aatgatgtta ctgtgggaat acttccaggt
     2341 gaaacattac aacttcagag agaactcact cttgatgaat cagatgatat gtactttatt
     2401 attgcaacag ctaaaactgc cggtttacaa ttaaatgtac atgtggaata taatgtgttc
     2461 agtaataatc catgggtaaa cggggatgaa atgtgggttg atattccggg agtggcatat
     2521 attgtatata cggcagttga tccatcaaat gatatttcat ttgcgggagg tttcaggaac
     2581 tggtactatg gaagatggaa tgcggattca tatatgtatg gaaacgatgc cccggacaca
     2641 tacgattatg aatttgagcc gatacaaact gatcttatga aaattaatgt tgatgaggca
     2701 tcaaacaatc cacagaattt tgcaaagaaa ttaacaattt gttcttccat gattccaaaa
     2761 tacaaagaaa aaacagatga tgaactacag gaagagatat ccaaagaaaa cccccatacc
     2821 actgatccat catttcctgt atggaggggt atggatgatg attgctggat aggtcccgga
     2881 gtttttgcaa caacgcgggt ggctaagaaa aatatagagg atttattgca tgccagtgat
     2941 acacaggtga tagaaacaga attattgcaa accagagcac tgaattcagg taatactggt
     3001 gtagttgata aacgtgggat aacaaaaata acaaaaacgt ataatgatac agtaagtttc
     3061 tcaggacgtg gttcgtcagt aacagttact accagtacag gttacaccaa aatacaattt
     3121 tttgatgtta atggtgatag atacccagat atacttactg ataatggact ggtatttaca
     3181 ggtattaacg ggttatatgc aggtatgaca ggtgctttta gtggtagtgt tcaaaagttc
     3241 gtaagcacga attataattt gggaatatgc ccacagtcgt ctgcaagcca ggttgagact
     3301 attccgcaag gagatggata tattgttaaa gttaaaacat cattttcaaa tctatctttt
     3361 tcattagcaa caggagacaa caatattatt gttgattatc aggatatcaa tggtgatgga
     3421 ttaccggaca ggattgaata taatggtttt gcaggctatt cagtacggta taacacgggt
     3481 tatggtttat caccaaagtg tgcttttaaa aatctttatg gtatacagca tactaagaat
     3541 aaaagtagat cgttgagtgt ggggtttagt actgataaat caattataag tggtggagta
     3601 tcggttgtgg aaaatagcag tgagaagctg gatgttccat tacttaatga aggggataga
     3661 tatacgctta ttgatataaa tggtgatgga ttacctgata gaatagtgga atatgatcat
     3721 ggaatatata tatttgttaa tacaggtgaa ggatttgtct tatatgctaa aaaaagtgat
     3781 gggaaaatgg atgaaactaa aggaatgtca tgtactccat cagttactct tggtgtaaaa
     3841 tttacaattt tgccactagt aactatagga acaatcatta tagatggtgg tgtaagctta
     3901 acaaaagatg gcaattctac ccgttacgct cttcgtgaca tcaatggtga tggattaccc
     3961 gatatcattt atgatgataa tggcacattg aaagccaaat taaaccgtac aggcaagacc
     4021 aatatgctta aatccattaa aaattccatt ggcggtacaa tagagcttga ctacaaacgg
     4081 gtgggcaata cccgtct
//
LOCUS       DAORYL010000147         4050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_14843_length_4050_cov_11.365457, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000147 DAORYL010000000
VERSION     DAORYL010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14843_length_4050_cov_11.365457"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PLV81_14925"
     CDS             <1..721
                     /locus_tag="PLV81_14925"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOM11432.1"
                     /translation="ITIKTTIDELKNNISYILGDSHNETKLMVQDVNLSLVKIEEFNP
                     AFSEKVVAGNPELQLLSIQVLIDEEAANLKENELYPDVEVGVSYMQRDKTPQGVNRDD
                     MFSVMATFNIPVWFMSKNIPAIQEMKIKKGESEALLKDKQNEITFALATLRQNIEKWK
                     QLDELYSQGVIPQLHSMLQADIANYRTGVAEFMKLIDSIRMMLHYKQQQLNTIKEYCK
                     AVSFLHFLLGDTSILNWSGVQ"
     gene            718..1977
                     /locus_tag="PLV81_14930"
     CDS             718..1977
                     /locus_tag="PLV81_14930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOM11433.1"
                     /translation="MKKLFIIIIVIIIVIAAIVFFFSNYRQNISHKDTEAVTYTCPMH
                     PQITSDKPGSCPICGMDLVPVEEEKKNESHKEHQEITGYTSILLDEKARILGITYETV
                     GIKDIVKILRTSATIVANEEKTYKISFRISGWVDKLFVNKMGQFISKGEPLLSLYSPE
                     LYAAVSEYISIINSIETMSNNQTVADTLVNLKKSSKEKLLLYGLTEKQVNEIETKKEA
                     TRTVTIYSPYTGYIIDKNVFEGQKVNSNEILMTIVDLTTVWAIAEVYQPDLLYIKPGM
                     PATLSISYWGNKEYRGNVQFVYPFVNEQTRTVKVRAVFYNNALELKPGLYGEIMLRYS
                     AGKHLAISEQAVFKTGEKNYVFVKHGDDTLMPQEVVLGALGDNGYYEVQKGVRKGDVI
                     VSPANFLIDSESQLKAVFSNVKEGHVH"
     gene            2000..>4050
                     /locus_tag="PLV81_14935"
     CDS             2000..>4050
                     /locus_tag="PLV81_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOM11434.1"
                     /translation="MIKKIISFSVHNKALVLFVISFLLLYAVYAIENIPLDAIPDLSD
                     TQVIIYTRWDRSPQIVEDQVTYPIVSALLGAPNVKTIRGFSDYGFSYIYVIFEDGTDI
                     YWARSRVVEYLSKIQGSLPQDVKMELGPDATGVGWIYQYALIDKSGKYDLSQLRTLQD
                     WYIKYSLQSVPGVAEVATVGGFEKQLQVTINPNTLHSYNISLLDIIEAIRKSTNEMGG
                     RLIEWSGKEYMVRAEGYIENINALKKVVVKYNIPTPVLLEHVADVTVGPQIRRGIAEF
                     NGEGEVVGGIIVMRHKENALNVIKKVKEKIQEVKKGLPEGVEIVPVYDRSGLIKQSIS
                     TLTGALIEEMIVVSIVILIFLWHIPSALVPIITIPISVILAFIPMYFMKITSNIMSLT
                     GIAISIGVLVDGAIVEVENAYKKIEHWIANGRKGDYHAVRLEALMEVGPSVFFSLLVI
                     TTAFLPIFTLVDQEGRLFTPLAWTKTLTMALAAILAITLDPAVRMLFTRLEPYKFKSK
                     KLSKAFTTLFVGKYYAEEKHPISKTLFKIYEPACRFVLKYPKQVIVGSIFIFIITTPV
                     FFSLGSEFMPPLNEGTILYMPTTLPGISLTEARKLLLYQDEVLKSFPEVESVFGKAGR
                     AETSTDPAPLSMIETTVVLKPKEQWRKKEQWYSRWAPEPLQQLLRHVWPDHISYSELI
                     A"
BASE COUNT         1390 a          625 c          796 g         1239 t
ORIGIN      
        1 aattactatt aaaactacca ttgatgagtt aaaaaacaac atatcgtata ttcttggcga
       61 tagtcataat gaaactaaac ttatggtaca ggatgttaat ctttcccttg taaagataga
      121 agaatttaat cctgctttta gtgaaaaggt ggttgctggt aatccagaat tacagttact
      181 atctattcaa gtacttattg atgaagaagc tgcaaacctt aaagaaaacg aattatatcc
      241 tgatgtagaa gttggtgttt catatatgca gcgtgataaa actccgcagg gtgtaaaccg
      301 tgatgacatg ttcagtgtca tggcaacgtt taacataccc gtatggttca tgagcaaaaa
      361 cattcccgct atccaggaga tgaagattaa aaaaggtgag tcagaagcgc tactcaaaga
      421 taaacaaaat gaaatcactt ttgcacttgc aacattacgg caaaatattg aaaaatggaa
      481 gcagcttgat gagctgtatt cacaaggtgt tataccacaa ctgcattcaa tgctgcaggc
      541 tgatatagct aactatagaa cgggagttgc tgaatttatg aaattaattg atagcattcg
      601 catgatgttg cattataaac agcagcaact gaacacgatt aaagaatatt gtaaagcggt
      661 ttcattttta cattttttac tgggtgatac atcaatactt aattggtctg gtgtacaatg
      721 aaaaaattat ttatcataat tattgtaatt atcatagtta ttgctgcgat agtatttttt
      781 ttcagtaact atcgccaaaa catatcccat aaagatactg aagcagtaac atatacctgc
      841 ccaatgcatc cacaaattac ttcggataaa cctggtagtt gtccaatatg tggtatggac
      901 cttgttccag ttgaagaaga aaaaaagaat gagtcacata aggaacatca ggaaattaca
      961 gggtatacct ccatattgct tgatgaaaag gcacgtatac ttggcataac atatgaaaca
     1021 gttggcatta aagacattgt aaaaatattg cggacatcgg caaccatagt ggcaaatgaa
     1081 gaaaaaacat ataaaattag tttcagaatt tcagggtggg ttgataaatt atttgttaat
     1141 aaaatggggc agttcatttc aaaaggagaa ccattgcttt cgctttacag tcctgaactg
     1201 tatgcagcgg tcagtgaata tatttcaatt attaattcta ttgaaacgat gtccaataat
     1261 caaactgtag cagatacgct agtaaatctt aaaaaatcat ctaaagaaaa acttttactc
     1321 tatggcctta ctgaaaaaca ggttaatgaa atagaaacta aaaaagaagc cacaagaact
     1381 gtcacaatat attcaccata tacaggttac attatagata aaaatgtttt tgagggtcaa
     1441 aaagtaaatt ccaatgaaat tttaatgacg attgttgacc ttacaacagt gtgggcaatt
     1501 gcagaggtgt atcagcccga tttgctatat ataaaaccgg gaatgccagc tacgttgagc
     1561 atatcgtact ggggtaataa agagtataga ggaaatgtgc aatttgtgta tccttttgtc
     1621 aatgaacaaa ctcgtacagt aaaagtaagg gcagtatttt acaataacgc attagagctt
     1681 aaaccaggat tgtatggtga aattatgtta cgatattctg cagggaaaca tctggctata
     1741 tcagaacagg ctgtgtttaa aacaggagaa aaaaactatg tttttgtaaa acatggtgat
     1801 gacacattga tgccgcaaga agttgtatta ggagctctgg gtgacaatgg atattatgaa
     1861 gtacaaaaag gggtcaggaa aggtgatgtt attgtgtcac ctgcaaattt cctgattgat
     1921 tctgaatcgc aattaaaagc tgtattcagt aatgtaaagg aaggccatgt gcattgatga
     1981 taaatcatgg taaggatgta tgattaaaaa aattatatca ttttctgttc ataataaagc
     2041 actggtatta tttgttatat cgtttttact gttatatgct gtctatgcta ttgaaaacat
     2101 tccccttgat gctattcctg atttatctga tacccaggtg ataatttata ccaggtggga
     2161 cagaagcccc cagatagttg aagatcaggt aacatatcca attgtctcag ctttattggg
     2221 agcaccaaac gttaaaacaa tccgtgggtt cagtgactac ggattttctt atatttatgt
     2281 tatctttgaa gatggtaccg atatttactg ggcacgtagc agagtggttg agtatctttc
     2341 aaaaattcag ggttcattgc cacaggatgt taaaatggaa ctgggaccgg atgcaacagg
     2401 ggtgggttgg atttatcagt atgcattgat tgataaaagc ggcaaatatg atctttcgca
     2461 attaagaaca ttacaggact ggtatattaa gtattcattg caatcggtcc ctggagttgc
     2521 tgaagttgca acagttggtg gttttgaaaa gcaattacag gttactatta atccaaatac
     2581 attacatagt tataatataa gtttacttga tatcatagaa gcaataagga aaagcacaaa
     2641 tgaaatgggt ggtcgcctca ttgaatggtc tggcaaagaa tatatggtga gagctgaagg
     2701 atatatagaa aacattaatg ccttaaaaaa agtcgtggtt aaatacaata tccctacacc
     2761 tgttttattg gagcatgttg ctgatgttac tgtagggcca cagattcgta gaggtattgc
     2821 tgaattcaat ggagagggtg aagttgttgg gggaatcatt gtaatgcgcc ataaagaaaa
     2881 tgcccttaat gttatcaaaa aagtaaaaga aaaaattcaa gaagttaaga aaggcttacc
     2941 tgaaggagtg gagatagtgc ctgtgtatga taggtcaggt ttaattaaac aatcaatttc
     3001 tacattaaca ggtgcactta tagaagaaat gatcgttgtg agcatagtta ttctgatatt
     3061 tttgtggcat attccatcag cccttgttcc aatcataaca attccaatat cagttattct
     3121 tgctttcata ccaatgtatt ttatgaaaat cacttccaat attatgagtt taacagggat
     3181 tgcgatttct attggtgttc ttgttgatgg agcaattgta gaagttgaaa atgcatataa
     3241 aaaaattgaa cactggatag caaatggaag aaaaggagat tatcatgcag tacgtttaga
     3301 agcacttatg gaagttggac cgtctgtgtt tttttcatta ttagtgatta caacagcatt
     3361 tttacctatt tttactcttg ttgatcagga aggaaggctt tttacgccac ttgcatggac
     3421 caaaacgtta accatggcac tggcagccat attagctata acacttgatc cggcagtacg
     3481 aatgctattt acacgattag aaccgtataa atttaaatca aaaaaattgt caaaagcttt
     3541 taccacattg tttgttggga aatactatgc tgaagaaaaa catccaataa gtaaaaccct
     3601 tttcaagata tatgaacctg cgtgtcgatt tgtattgaag tatccaaaac aagtaatagt
     3661 tggttcaatt tttattttta ttatcacaac accagtcttt ttttcactgg gttcagaatt
     3721 tatgccacct ttaaatgaag gtactattct ttatatgcct accacattgc caggtatatc
     3781 acttactgaa gccagaaaac ttttgttgta tcaggatgaa gtgttaaaaa gctttcctga
     3841 ggttgaatca gtatttggca aagcaggtag agcagaaact tcaactgatc cggctccttt
     3901 atctatgatc gaaactactg tagtacttaa accaaaagaa cagtggcgga aaaaagaaca
     3961 atggtattcc cggtgggctc cagaaccact acaacaatta ttacgtcatg tatggcctga
     4021 tcatatatcc tattctgaac tcattgcaat
//
LOCUS       DAORYL010000148         4049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_14847_length_4049_cov_14.626690, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000148 DAORYL010000000
VERSION     DAORYL010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14847_length_4049_cov_14.626690"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PLV81_14940"
     CDS             complement(<1..294)
                     /locus_tag="PLV81_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11435.1"
                     /translation="MIIIAIKNVLLPDTVYDTIYSGDFDKCKVYDLEATMKLIEYFID
                     SSSAYLEDNKNKKMRIFYNDKKVMNYINKIYKNMIDKYKLILYLSKDPKNSHWN"
     gene            355..1542
                     /gene="nhaA"
                     /locus_tag="PLV81_14945"
     CDS             355..1542
                     /gene="nhaA"
                     /locus_tag="PLV81_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009669618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HOM11436.1"
                     /translation="MKENNQSFLHQFFAMESASGMLMIIATILAMIIANSPMKLLYTM
                     MLELRFAVSIGDFAIDKPLLLWINDGLMAIFFFMIGLELKRELVEGELSNIKKVALPA
                     IGAVGGMLVPALIYAAFNYSDPQALKGWAIPAATDIAFSLGILSLLGNRVPLSIKIFL
                     TSLAIFDDLGAIIIIAFFYTANISLTALAVSLVCIPVLLFINKKGITSKSIYVIIGIV
                     MWAAMLKSGVHATLAGVILALCIPLTSKDATSPLREFEHDVHHAVAFIILPVFAFANT
                     GIDFSKMTAQHLLHGVPIGIAAGLFIGKQAGISIFCYIAWKLGIASLPSQTNWKMLYG
                     TALLCGIGFTMSLFIGSLAFEETGSNLLFDERIGILAGSLLSGIFGYLVLHKALPEKL
                     DKA"
     gene            1824..2525
                     /locus_tag="PLV81_14950"
     CDS             1824..2525
                     /locus_tag="PLV81_14950"
                     /inference="COORDINATES: protein motif:HMM:NF016277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11437.1"
                     /translation="MEISGRSFIANPEECPYLYNRTKTYRYTVLYNLTPDEHNTLLAQ
                     GWRHFGYTFFQPACTSCMLCIPVRIKTFDFIPTKSQRRVLRKNANTLTRINELTLTRR
                     IYNIYEDHSISRFKELPSLQFLLSNVLDVCANTYQIEYYHNSQLFAVDYIDETREALS
                     SIYCIYHSDFAYLSPGIYSILYAIELARQKRLPYFYLGYYVPGCNVMQYKISFHPYQW
                     FNWEKQKWCDEPVIY"
     gene            complement(2528..2866)
                     /locus_tag="PLV81_14955"
     CDS             complement(2528..2866)
                     /locus_tag="PLV81_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11438.1"
                     /translation="MNSKTQFDIAINERLQSNSWDETIASAVVARVKKRNRLYIACGA
                     IAALFMIVVGISLWQGSQYPAVTTYSFIHAQANGVFKNVFPEAQSTDLTYSYIDEHVT
                     DIIDTALLER"
     gene            complement(2863..3354)
                     /locus_tag="PLV81_14960"
     CDS             complement(2863..3354)
                     /locus_tag="PLV81_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOM11439.1"
                     /translation="MNDKEFAKIIGHTKSVVLQAVYRHLAAQFYEAIDDVVQETYIRA
                     YRSLVSEKFKEKSSLETWLYRIAANEAKRMNKKLLREEQKLQKLSHSMDCNPPEIQYD
                     ELSLMPVIEKLPEKYKDVMKLKALGNDETQIAGKLGIPKGTVKSRLSRGKLILYKLLQ
                     EEV"
     gene            complement(3372..3863)
                     /locus_tag="PLV81_14965"
     CDS             complement(3372..3863)
                     /locus_tag="PLV81_14965"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOM11440.1"
                     /translation="MKKVFMYALALTLVASVAFAQGPKGKGQCCDMPMKKGPIFGDVE
                     RLKQELGLSDAQVDKIVEINSLYEKRMLDIREKLAPKMIQLKKLLLEDEVDINAVRAK
                     LKEIGDLRIELHILRIQHVMDIEKQLNKDQLKKLKAYKQMEMQRMEKMRPHGPMGFDE
                     PMM"
BASE COUNT         1187 a          840 c          651 g         1371 t
ORIGIN      
        1 gttccaatga ctatttttag gatctttact aagatatagt attaacttat acttatctat
       61 catatttttg taaattttat ttatgtagtt catcactttt ttatcattat aaaaaatcct
      121 catctttttg ttcttattat cttctaaata tgcacttgag ctatcaataa aatactctat
      181 caatttcatt gttgcttcta aatcataaac tttgcattta tcaaaatcac cactataaat
      241 cgtatcatat acagtatctg gtagtaaaac atttttaatt gcaataatta tcactataag
      301 atatgattca attatatcat aaaagccatt acatgtaaaa ggagttgatt cctcatgaaa
      361 gaaaataacc aatcattctt acaccaattc tttgcaatgg aatctgcaag cggcatgttg
      421 atgatcattg caaccatact ggctatgatt attgccaatt cacctatgaa gctattatat
      481 acaatgatgc ttgaacttcg ttttgcagta tccattgggg actttgcaat tgacaaacca
      541 cttttgctat ggattaacga tggactcatg gcaatttttt tcttcatgat tggccttgaa
      601 ctaaaaagag aacttgttga aggggaacta tcgaacataa aaaaagtagc actgcctgct
      661 attggcgcag ttggaggcat gctggttcct gctttaatat atgcagcgtt caattatagt
      721 gacccacaag cgttaaaagg ttgggcaata cctgcagcca ccgatattgc attctcgtta
      781 ggcattcttt cattacttgg taaccgagta ccattaagca tcaagatatt tttaacttca
      841 cttgctattt ttgatgatct gggtgcaatt attatcattg cattttttta tactgcaaac
      901 atttctttaa ctgcccttgc tgtatcgctt gtatgtattc ctgtattatt gttcatcaac
      961 aaaaaaggca tcacctcaaa aagcatatat gtaattatag gaatagtaat gtgggcagca
     1021 atgctgaaat ccggtgtcca tgcaacactg gctggggtta tactggcatt atgtattcct
     1081 ttaacatcaa aagatgccac ttcaccactg cgagaatttg aacatgatgt acatcatgct
     1141 gtagcattta ttatattacc agtatttgca tttgccaata ctggaattga ttttagcaag
     1201 atgacagcac agcatcttct acacggagta cccattggca ttgcagcagg tctttttatt
     1261 ggtaaacagg caggcatatc tatcttttgc tacatagcct ggaaacttgg tatagcatca
     1321 ctgccatctc aaaccaactg gaagatgctg tatggaactg cattactatg tggcataggt
     1381 ttcaccatga gtctttttat tggctcactg gcttttgaag aaacaggcag caatctttta
     1441 tttgatgaac gcatcgggat tctggcaggg tcactgctgt cagggatttt tgggtatctt
     1501 gtgttgcata aagcacttcc tgaaaagtta gataaagcat aaaaagtcaa tgcatgtaat
     1561 cttcacttgt agcgtctgaa gaatcaattt ccttctttaa aattaagatt gccatgaccc
     1621 tcctgggctc gcaatgacat aaatataata actttataga ctattttttt tattctggtt
     1681 ggtcacagtt gtggtcagga gcaatgtgct aaatacttaa tgattacatg gttaagctaa
     1741 aaattttttt cttaaaaaaa ctttatgttc agccttcctt aaattattga caatttatac
     1801 attttcacaa ttatactatc agcatggaaa tttcaggaag atcattcata gccaatcccg
     1861 aagaatgtcc ctatctttat aatagaacca agacatatcg ctataccgta ctgtacaatc
     1921 tcacaccaga cgaacacaac acacttttag cacaggggtg gcggcatttt ggttatacct
     1981 tttttcagcc agcgtgcaca tcatgtatgc tatgcatacc tgtaaggatt aaaacatttg
     2041 attttatacc cacaaaaagt cagcgcaggg ttttacgtaa aaatgcaaac acgcttactc
     2101 gcatcaacga attaaccctt acacgaagaa tttacaacat atatgaagac cattccatat
     2161 cccgttttaa agagctacca tcactacaat ttttattgtc aaatgtactt gatgtatgtg
     2221 caaacaccta ccagattgaa tactatcaca actcacaact gtttgccgtg gactacattg
     2281 atgaaacacg tgaagcatta agttccattt actgtatcta tcactcagac tttgcttact
     2341 taagtcctgg aatttatagt attctgtatg ccattgaatt agcccggcaa aaaaggctgc
     2401 catactttta tttaggctac tatgttcctg gctgcaacgt tatgcagtac aaaatatcat
     2461 ttcatccata tcaatggttt aactgggaaa aacaaaagtg gtgtgatgaa ccggttattt
     2521 attaaagcta tcgctccagc aatgccgtat caataatatc tgttacatgc tcatcaatat
     2581 agctgtatgt caggtcagta gattgtgctt cagggaaaac attcttaaac acaccatttg
     2641 cctgtgcatg gataaagcta tacgtggtaa cagccggata ctgtgacccc tgccacaaag
     2701 aaattccaac cacaatcatg aaaagtgcag caatagcacc acatgcaata tacaatctat
     2761 ttcttttttt gacccgtgct actactgctg aagcaattgt ttcatcccac gagttacttt
     2821 gcaatcgctc attgattgct atatcaaatt gtgttttact attcatactt cctcctgtaa
     2881 caacttataa agaatcagtt tccctctgga taagcgcgat tttaccgtac cctttggaat
     2941 acccagcttc cctgcaatct gtgtttcatc attacccaga gctttcagtt tcataacatc
     3001 tttatacttt tcagggagct tttcaataac aggcataagc gatagttcat catattgaat
     3061 ctcaggagga ttacaatcca tactatgtga aagcttttga agtttttgct cctctcgcaa
     3121 aagcttctta ttcatacgtt ttgcctcatt tgctgctatg cgatacaacc aggtctcaag
     3181 agatgacttt tctttgaatt tttctgaaac aaggcttcgg taagcccgaa tatacgtttc
     3241 ctgtactaca tcatctattg cctcataaaa ctgtgctgca aggtgcctgt acacagcctg
     3301 cagcacaact gattttgtgt ggccaataat ttttgcaaac tctttgtcat tcatgttata
     3361 agccttaacg tttacatcat tggttcatca aatcccattg ggccatgtgg acgcattttt
     3421 tccattcgtt gcatttccat ctgtttgtaa gcttttagct tctttagctg gtctttgtta
     3481 agctgttttt caatatccat aacatgctgt attctcaata tatgaagctc aatccgtaaa
     3541 tcgcctatct ctttcagttt agctcgcact gcattaatgt caacttcatc ttcaagcaaa
     3601 agctttttta gctgaatcat tttgggagct aatttttccc gtatgtcaag catgcgcttt
     3661 tcatacagac tattgatttc aacaatctta tctacctggg catctgacaa ccccaattcc
     3721 tgctttagcc gctctacatc accaaagatt gggccttttt tcatgggcat atcgcaacac
     3781 tgccctttcc cttttggccc ctgcgcaaat gcaacactgg caacaagtgt cagagccaga
     3841 gcatacataa aaaccttttt catacaaatc cccctttaag tatagtagct tctcaaattt
     3901 tgaacattag acctttattc aaagctttta gttccatttt ttttgttata ttcttcattt
     3961 ttagctctga caccgcacct gcatacggga cttcatcttt ggcgtagtag tatcttgctc
     4021 tgacaccata ctttcacaaa gacttaatt
//
LOCUS       DAORYL010000149         4028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_14941_length_4028_cov_8.924239, whole genome shotgun sequence.
ACCESSION   DAORYL010000149 DAORYL010000000
VERSION     DAORYL010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14941_length_4028_cov_8.924239"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PLV81_14970"
     CDS             <1..273
                     /locus_tag="PLV81_14970"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOM11441.1"
                     /translation="FGIAVPHAKLESVHKIAFAIGISKKGIDFNSIDGKPVHLVVLVA
                     AGERQHKEYLKLLSKIMSVLKNDIIRNEMIQAKSTKDIIEILRNAK"
     gene            285..2051
                     /locus_tag="PLV81_14975"
     CDS             285..2051
                     /locus_tag="PLV81_14975"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOM11442.1"
                     /translation="MDKTLYILQHYYNSIKKFFTTRYDLKEYILQIGISLLIGTTAGL
                     GAMVFHTLIEWMRFAINPSRFSRYLEIPSFVMAIVPVIGALMIIGLTRLFPQQASERG
                     VTSVIKSVLLQKIPIPLFTTLFHLLASAVTLGTGAPLGPEGPSAKIGGGLGSYISSLF
                     RLNIRKMTTYTVAGAGAAISAIFNAPIAGVFFGIEVILLNDLKNESLSALVIASVSAD
                     MVSKAYLGNVALLQIPKFQVNTHEVLYFILLAIVCGVVALLFMELSHIIKKFYKNIRK
                     DDTIAILLPLSLLFAVAVMFYTDIYGIGYELINKVLAKEFSVNILVILFVLKIIFIAL
                     YFQTGAYGGLFAPSLCIGILVGYLFASMLSFFGATLDPVAYALIGMGGMLAGLNSIPI
                     TAILMVFELTNDYRIILPVMLASVIAHLTVTYYNKGTIYELELLEEGIDVRKSRINVL
                     ESVTVHSIARKDFQTINAKAPLKFAMDKLIEAETGLIVVDDNGGYTGILSLNEVRQAL
                     LSSDVVELLICQDIVLHVNPCSENNSAAEALRRMDEYGIDFLPVAKGNKVTAIVLYRD
                     IISMYNKIIVQIEEKQYGHFGL"
     gene            2200..>2588
                     /locus_tag="PLV81_14980"
     CDS             2200..>2588
                     /locus_tag="PLV81_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOM11443.1"
                     /translation="MQEKEYKPKQIEKKWQDRWNQEQVFLSKRDKNRQKYYILEMFPY
                     PSGRIHMGHVRNYSIGDVVARYKRMQGFNVFHPMGWDAFGLPAENAAIKNNIPPKKWT
                     DDNIAYMKKQFKMLGYSYDWEREIATHT"
     assembly_gap    2589..2688
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2719..>4028
                     /locus_tag="PLV81_14985"
     CDS             2719..>4028
                     /locus_tag="PLV81_14985"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOM11444.1"
                     /translation="MVIAPEHPFLERVKDSKVKEFIKRIKEQSLVDRLSEEKEKEGID
                     TGIKIINPFNGDIVPLYVGNFVLMEYGTGAIMAVPAHDTRDFAFAKKYGIPIKLVIDN
                     PSSPIDVASMKDAYVEDGICVNSGPFTGMTNKKAIEAISDYAEKEGIGYKKVNYRLKD
                     WLISRQRYWGCPIPIVYCETCGTQPVPYDNLPVVLPTDVDFHGDSRSPLTMMPEFYET
                     KCPHCGGKAKRETDTMDTFVDSSWYFAKFTSPRSKEIFDFDEVNYWTPVDQYIGGIEH
                     ACMHLLYARFFTMVLNDLGILKYREPFTRLLTQGMVIKDGAKMSKSKGNIVDPDDIID
                     KYGADTVRLFMLFAAPPEKDLDWSDKGVEGCYRFVNRVWRIVQKYSDCYTEYSFDTVV
                     LDPVLKNLRGEIHRTVKAVTHDIDNRMQFNTAIARMMELVNALY"
BASE COUNT         1207 a          627 c          835 g         1259 t
ORIGIN      
        1 tttggtattg ctgttccaca tgctaaactg gaatcagttc ataaaattgc atttgcaata
       61 gggatttcta aaaaaggcat agattttaat tctattgacg ggaagccagt acatctggtg
      121 gtactggtag cggcgggtga acgtcagcat aaagaatatt tgaagttatt gtcaaaaata
      181 atgtctgtcc tcaaaaacga tataatacgc aatgaaatga ttcaggcaaa gagcactaaa
      241 gatattattg aaatacttcg taatgccaag taattttttg ccacatggat aaaacactct
      301 atattcttca gcactattat aatagtataa aaaaattttt cacaaccaga tatgatctga
      361 aagaatatat attacagatt ggcatttcgc ttttaattgg aactactgca ggcctgggtg
      421 ccatggtgtt tcataccctt attgagtgga tgcgttttgc aataaaccca tcacgttttt
      481 cgcgctacct tgaaattccg tcatttgtta tggcgatagt tcctgttatc ggggcgctga
      541 tgattatagg cttaacacga ttatttccgc agcaggcgtc tgaacgaggt gttaccagtg
      601 ttattaaatc tgtcctgtta caaaaaatcc caataccact ttttacaacg ttgtttcatc
      661 tattagcttc tgccgtgaca ttgggtactg gtgcgccact ggggcctgaa ggtccttctg
      721 ctaaaatagg tggtgggctt ggttcatata tttcatcgct ttttcgttta aatatccgta
      781 aaatgactac atataccgtt gcaggagcgg gtgctgcaat ttctgctata ttcaatgccc
      841 ctattgcggg ggtgtttttt gggatagaag taatattact caatgattta aaaaatgagt
      901 cattgagtgc tctggttata gcatcggtat ctgcagatat ggtttcaaaa gcatatctag
      961 gaaatgttgc attgttacag atccccaaat ttcaggttaa tactcacgaa gtcctgtatt
     1021 ttattttact ggcaatagta tgtggtgttg ttgcgctttt gtttatggaa ctatcgcaca
     1081 taataaaaaa attttataaa aatataagaa aggatgacac aatagccata ttgttaccac
     1141 tatcgctgct atttgctgtt gcggttatgt tttatacaga catctatggc atagggtatg
     1201 aattaatcaa taaagtcctg gcaaaagaat tttcggtaaa tattctggtg atattgtttg
     1261 tgttaaaaat tatatttata gctctgtatt ttcaaacagg agcctatggc ggtttatttg
     1321 ctccatcgtt atgtattgga atactggttg gttatttgtt tgcatcaatg ctttcttttt
     1381 ttggggcaac acttgaccct gttgcgtatg cactcatagg tatgggtggt atgcttgctg
     1441 gtttaaactc aataccaatt actgccatat tgatggtatt tgaattgacc aatgattata
     1501 gaatcattct tcctgttatg cttgcttcgg ttatagcaca ccttactgtt acttactata
     1561 ataaaggcac tatctatgaa ttagagttgc tggaagaagg tattgatgtt cgtaaaagcc
     1621 gcattaatgt tcttgaatca gtgactgtcc attcaatagc tcgaaaggat tttcagacaa
     1681 ttaatgcaaa agcaccactc aaatttgcaa tggataaact gattgaagcg gaaacaggtc
     1741 ttattgtagt tgatgataat ggtggctata caggtatatt gtcattaaat gaagtcaggc
     1801 aggcacttct ttccagtgat gtggttgaat tactcatctg tcaggatata gtgttacatg
     1861 ttaatccctg cagcgagaat aattcagcag cagaggcgct aagaagaatg gatgaatatg
     1921 gaatcgattt tttacctgta gcaaaaggga ataaggttac agctattgtc ctgtatcggg
     1981 atataatcag tatgtacaat aaaattatag tgcagattga agaaaagcaa tatggacatt
     2041 ttggtttata atatatgtgc gatagttacc cgctaacaaa atacttgacg ataaaaccag
     2101 cgtgaataat gtgcatatga attgaattaa taagtattgg tagatactat tgatgtactg
     2161 gtagcaactg ttgtaaaatg tattattgga ggatgatgta tgcaggagaa ggaatataaa
     2221 cccaagcaaa ttgaaaaaaa gtggcaggac cgatggaatc aagaacaggt ttttttatca
     2281 aaacgtgata aaaacaggca aaagtattat atattagaaa tgtttccgta tccatctggc
     2341 agaatacaca tgggccatgt gcgcaattat tcaattggag atgttgtagc tcgttataaa
     2401 cgaatgcagg gctttaatgt attccatccc atgggatggg atgcatttgg cttaccagca
     2461 gaaaatgctg ctatcaagaa taatattcct cctaaaaaat ggacggatga caatattgcg
     2521 tatatgaaaa agcaatttaa gatgttgggg tactcttatg attgggaaag ggagatagca
     2581 acccatacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg tccagatact
     2701 atttacggtg tgacttttat ggtaatagct cctgaacatc catttctgga gcgagtaaaa
     2761 gactcaaaag taaaggaatt tattaaaaga attaaagagc agtctctggt agacagactt
     2821 tctgaagaaa aggaaaaaga aggtatagat accggaataa aaattatcaa tccgtttaac
     2881 ggtgatattg tgcctttata tgttggtaat tttgttctca tggaatatgg tacgggtgct
     2941 attatggctg tcccggcaca tgatactcgt gactttgctt ttgctaaaaa atatggcatt
     3001 cctataaagc ttgttattga taatccttca tcgcctattg atgttgcatc aatgaaagat
     3061 gcgtatgttg aggatggtat atgtgttaat tccggtccct ttacaggtat gactaataaa
     3121 aaggccattg aagcaatatc agactatgct gaaaaagaag gtattggtta taaaaaagta
     3181 aattatcgcc taaaggactg gttaatatct cggcaacggt actggggctg cccaatacca
     3241 attgtatatt gtgaaacctg tggcacacaa cctgttccgt atgataactt accggtagta
     3301 ttgccaacgg atgttgattt tcatggagat tctcgttcac ctcttaccat gatgcctgaa
     3361 ttttatgaaa ctaaatgtcc gcactgtggc gggaaagcaa agcgtgaaac agataccatg
     3421 gatacttttg ttgattcttc gtggtatttt gcaaagttta cttctcctcg ttcaaaggaa
     3481 atctttgatt ttgatgaggt caattactgg actccagttg accagtatat tggtggaata
     3541 gaacatgcct gtatgcattt gctgtacgca cgctttttta ccatggtcct gaatgatctg
     3601 ggaattttga aataccgaga gccttttacc cgtttgctga cacagggaat ggtaataaaa
     3661 gatggcgcaa agatgagcaa atccaaaggc aatattgtgg atcctgatga tattatagac
     3721 aagtacggtg ctgataccgt acgacttttc atgctttttg ctgctcctcc tgaaaaggat
     3781 ctggactggt cagataaagg ggttgaaggt tgctatagat ttgtaaaccg tgtatggcga
     3841 attgttcaaa aatatagcga ctgttatact gaatatagtt ttgatactgt tgtgttagat
     3901 cctgttttaa aaaatctccg tggtgaaatc catagaacag taaaggcagt gacacatgat
     3961 attgacaaca gaatgcagtt taatacggca attgcccgta tgatggaatt ggtaaatgca
     4021 ttgtatca
//
LOCUS       DAORYL010000150         4021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_14975_length_4021_cov_9.745335, whole genome shotgun sequence.
ACCESSION   DAORYL010000150 DAORYL010000000
VERSION     DAORYL010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14975_length_4021_cov_9.745335"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      446..586
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            648..2003
                     /locus_tag="PLV81_14990"
     CDS             648..2003
                     /locus_tag="PLV81_14990"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOM11445.1"
                     /translation="MGIPKSFWKSISPFQLLVSGYLLITLGGAFLLSIPIATKSGRFE
                     NYFNALFMASSGISTTGLCVVDPGSFYTFFGQVVLMVIFQIGGIGYMTLLMFILYLFD
                     IKTSLTVQLTARESLAGASLRVLGKFFIATVIFTLIFEMGGALLLALYWLKYYNLVHA
                     VWYGIFHSISAFCTAGFSLFPDNLIKYRDSIVVNAVIDIVSIAGGLGFIVLYEAVIFI
                     VNKYHNIKKLRLSIHTKLVFITTLILLMGGTFLVLITEKWDGSIDYIHRFMYSSFQVI
                     SAHTTDGFNTIDIGRMSSASLTVIMMLMFIGASPGSTGGGIKTTTFAITAKYIISLLK
                     GKSSGVVNIYEREIPQESVNKALGIFILFIVIIAIDVTIMSMTENQDFRNILFEIISA
                     LGNTGLSCGITQQLTLIGKLLLATTMFIGRVGPVTFGYFVFGSVKNTGYHYPQEDVFV
                     G"
     gene            2309..2938
                     /locus_tag="PLV81_14995"
     CDS             2309..2938
                     /locus_tag="PLV81_14995"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOM11446.1"
                     /translation="MPFYKFIPHEVKFFDLFDKQADKMVEAMNCFKEFAEKGIFDDTC
                     IEHMRVIEHESDGITHEIIDSLNKTFITPFDREDIHSLANELDDVVDLIYAISKRMRL
                     YKLKGKNKDIIEFSHLIMRSVSSLAGAIKGLRNHRQPQQIQEACIEINKIENISDQLR
                     DAVIQKLLESKKDPITIIKWKEIYELLETVLDVCEDVSNIVVSILVKQG"
     gene            2945..3955
                     /locus_tag="PLV81_15000"
     CDS             2945..3955
                     /locus_tag="PLV81_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOM11447.1"
                     /translation="MTLYIVFLIALALVFDFLNGFHDAANSIATIVSTRVLSPRYAVI
                     WAAFFNFIAFLVFGLHVAGTVGSGIIDINIIDNTIILATLTGACLWNIITWYLGLPTS
                     SSHALIGGLIGAALVKVGVNALMFDGIMKTVIFIFVSPIVGFILGLMFGIAIHWIFQH
                     AHPARVDKLFRKGQLISAAFYSLGHGGNDAQKTMGIIAGLLFSNGLMHIAHGQRFQDA
                     IPLWVVISCQTAIALGTMFGGWRIVKTMGQRVVKLRPVDGFCAEFGAAFNLFVITSYF
                     GIPVSTTHTITGAIMGVGSLKRFTAVRWGVAGQIVWAWVLTIPLAAIISGLTYFVAAH
                     WL"
BASE COUNT         1230 a          551 c          826 g         1414 t
ORIGIN      
        1 ccttttgcaa aagaggctga agtaataatt tgtgcgatag ttctaatgat tgttcattga
       61 tagccatagt agccattttt tatatagata tatatttacc aacaatgtat tgtgtatata
      121 tactttttca atctcttttt ttcaaattta caagaacaat ccacaggagg ttaaatatta
      181 aaagctaccg gtgagatgtg gagttttgtg acacgataat ttctcttagc ttgcaaaaga
      241 tgtaatgaaa tcattgttgc tttttcataa attatgatgc tgagaataaa aaagcatggt
      301 tacgtataac gcattataac aaaatgtgta tagctacatc actttaataa ttgaattaat
      361 ttcttttaag aataaatata aaaaataata tttattattg acgagataaa gaatttaaag
      421 tatattaata gccaatttta catgctgcat aaaaaagcct aaatccatta cagcagcaat
      481 ggatgcgggg gacccaattt ttggggcgta ttccttttac ataaggatag ggtacttcca
      541 aacccgagcc cgtcagctaa cctcgtaggc ttttggaagg gcaagagcac tataaaactg
      601 ctttttgcgg ttttttttat gcctttgccc cgcataggag gggtaatatg ggcattccaa
      661 aaagcttctg gaagtctatt tcaccattcc agttattagt ttcaggatat ctattgatta
      721 cactgggtgg agcattcctg ttatcaattc caatagcaac taaaagtggt cgctttgaaa
      781 attattttaa tgccttattt atggcatcct caggtataag tacaacaggt ttgtgtgtgg
      841 ttgatcccgg gagtttttat actttttttg ggcaggttgt attaatggtt atattccaga
      901 ttggcggaat agggtatatg acattgctta tgtttatatt gtatctgttt gacataaaaa
      961 catcattaac agtacaattg actgccagag agtcgttagc aggtgcatca ctgagagtgt
     1021 tgggtaagtt ttttattgca acggtcattt ttacgctaat ctttgagatg ggaggtgcat
     1081 tattactggc attatactgg ttaaagtatt ataatttagt tcatgctgta tggtatggta
     1141 tatttcattc aatttcagct ttttgtacag caggatttag tttattcccg gataatttaa
     1201 taaaatatag agatagtatt gtagtaaatg ctgtgattga tattgtatca attgctggtg
     1261 gacttggttt tattgtcctt tatgaagcag taatatttat tgtcaataaa tatcataaca
     1321 ttaaaaaact tcgtttatcg attcatacaa agctggtatt cattactacc ttgatactct
     1381 taatgggggg tacattcttg gtattaataa ctgaaaaatg ggatgggtct atagattata
     1441 ttcatagatt tatgtattcg tcatttcagg taatatctgc acatacaact gatggattta
     1501 atactattga tattggtagg atgagttctg ccagccttac agtgataatg atgttaatgt
     1561 ttattggtgc ttcgccaggt agtacaggtg gtggaattaa aacaacaact tttgctatta
     1621 cagctaagta tattatttca ctgttaaaag ggaaaagcag tggtgtggtt aatatttatg
     1681 aaagagagat tcctcaggaa tcagtgaata aagcactggg tatttttatt ttatttatag
     1741 taataattgc aattgatgta acaataatga gtatgacgga aaaccaggat ttcagaaata
     1801 tattgtttga aataatctct gcattaggca atacagggct ttcttgtggt attacacaac
     1861 aattgacatt aattggcaaa ctattgttgg ccactacgat gtttattgga agagttggac
     1921 ctgttacatt tggttacttt gtatttggta gtgttaaaaa tacagggtat cactatccac
     1981 aagaagatgt attcgttgga taaactggtg gtacaaaagt attattctat gaaggaatga
     2041 taatacaata ttccatgtgt tttggtttga catcatcaat tatttttaga aaacattttt
     2101 acataatgat agtgtacact tctactggag agtaggtcga ggatgtatac tattatattt
     2161 cctgttaata atattatatc ctgaaataat tggtggttta atatacatat taaaatatat
     2221 tgacaaaagg tttaattata tgaattgtcg ttttgtttat tatgtttctt aattttgtaa
     2281 taagctaaac atttatggag aatacgatat gccattttac aagtttattc ctcatgaggt
     2341 aaaatttttt gatctatttg ataaacaggc agacaaaatg gtagaagcta tgaactgctt
     2401 taaggaattt gctgaaaagg ggatttttga tgatacatgt attgagcaca tgcgtgttat
     2461 tgagcatgaa agtgatggaa ttacccatga gattattgat agtttgaaca aaactttcat
     2521 tacccctttt gatagagaag atatccactc gctggcaaat gaacttgatg atgtggttga
     2581 cttaatctat gcaatttcaa aacgaatgcg cctgtataaa ttgaaaggaa aaaataagga
     2641 tattatagaa ttttcacatt tgataatgcg ttcagttagc tcattagcag gagcgattaa
     2701 aggtttacga aatcatcggc aaccgcaaca aatacaggaa gcatgtattg aaataaataa
     2761 aattgaaaat ataagcgatc agttgcgtga tgccgttata caaaaattac tggaaagtaa
     2821 aaaagatcca ataactatta tcaaatggaa agaaatttat gaattactgg aaacagtgct
     2881 ggatgtgtgt gaagatgtat ccaatattgt ggtttccatt ctggtaaaac agggataaat
     2941 agtcatgaca ttgtatatag tatttttgat tgcacttgcg ttggtatttg atttcctgaa
     3001 tgggtttcat gatgcggcca attcaatagc aacaatcgtt tctacaaggg tactatcgcc
     3061 ccgatatgct gttatatggg cagcattttt taatttcatt gcttttcttg tttttggatt
     3121 gcatgtagca ggtacggttg ggagtggcat tattgatatc aatatcattg ataatacaat
     3181 tatactggct acgttaaccg gtgcctgcct gtggaatatc attacatggt atttaggttt
     3241 gcctaccagt tcgtcacatg cactcattgg agggttaata ggtgctgcgc ttgtgaaagt
     3301 tggcgtaaac gcattaatgt ttgatgggat aatgaaaact gttattttta tttttgtttc
     3361 gccgatagtt ggctttatat taggtcttat gtttggaatt gccattcact ggatttttca
     3421 gcatgctcat cctgcgcgtg ttgataagtt attcaggaag ggtcagttaa tatcagcagc
     3481 gttttacagt ttaggacatg gtggcaatga tgcgcagaaa acgatgggta taattgcagg
     3541 attgttgttc agcaatggtt taatgcatat tgcacatggt caaagatttc aggatgcaat
     3601 accattatgg gtagtcattt catgccaaac tgcaattgca ttgggtacca tgtttggggg
     3661 atggcgtatt gtgaagacga tgggacaacg tgtagttaaa ttaagacctg ttgatgggtt
     3721 ttgtgctgaa tttggcgctg catttaatct gtttgttata acttcatact ttggcatacc
     3781 agtcagtaca actcatacca taacgggggc tataatgggt gtggggtcac taaagcgatt
     3841 tacagctgtg cgatggggtg ttgccggcca gatagtgtgg gcatgggtac tgactatacc
     3901 actggcagcg ataatatcag gattaacgta ttttgttgct gcacattggt tgtaatatta
     3961 ttcctgttac aatcgtgtga tatcactctt tggatacatc aggtctataa tatctttata
     4021 t
//
LOCUS       DAORYL010000151         3917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_15495_length_3917_cov_11.912222, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000151 DAORYL010000000
VERSION     DAORYL010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15495_length_3917_cov_11.912222"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..447)
                     /locus_tag="PLV81_15005"
     CDS             complement(1..447)
                     /locus_tag="PLV81_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HOM11448.1"
                     /translation="MTRPTWDEYFMMIAHDVSTRATCIRRKVGAVIVKDKRILSTGYN
                     GVPTGITHCTPETCLRNIYNVPSGERHELCRGLHAEQNAIIQAAFHGVSIAGAIIYVT
                     HQPCSICTKMLINAGIKTFIFEHPYNDELAKAMMDEAGVEAIVFKR"
     gene            complement(444..1019)
                     /locus_tag="PLV81_15010"
     CDS             complement(444..1019)
                     /locus_tag="PLV81_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11449.1"
                     /translation="MQEKAKEYIEYIKKGEMPPDNNQRKKKFSRIFLLINIIVFIILL
                     TLIIQKKEPTDYIYQTVAVNGVQVRFSIIHTINQPLLASVTFTSNTSSQLSVKNPPVT
                     VTIAHNNNEILSQSFEYSSTMLAITPQKPVTLTAAILVEPVKEYAKKHDLLKPKRYVY
                     HSVIPLTVKFTLHTVMDYSVDLQLQCKVSAK"
     gene            complement(1024..1470)
                     /locus_tag="PLV81_15015"
     CDS             complement(1024..1470)
                     /locus_tag="PLV81_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11450.1"
                     /translation="MDIIATQQFIDTLRKLPDHSTLDREFFAPLYDFFEDAGASLLLS
                     TPDDYYLLYFDLPEAIDDILPTNVSWLVEKDEKTISLTMFADGKEIQTYHTFHFANNP
                     VNAYFFKSLQDSRTLTINFFAMMYGDIYKLKSIDFTLPQAILQQIE"
     gene            complement(1550..2227)
                     /locus_tag="PLV81_15020"
     CDS             complement(1550..2227)
                     /locus_tag="PLV81_15020"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOM11451.1"
                     /translation="MYTIHIFFLFAFGLFIGFISSIPVGAVQLEVIKKAINGHLKPAI
                     SVALGSVTSDFIYGVLVLFGIGGFLHYPKVQIFTYSLGILVIIALLYRSYREHKHIPP
                     SHKPHIQYKKRTSYLTGLTIAITNPGMIVWWVIGYKLLLDLQLFDTMNIPLKATFLVS
                     CCTGLAGYLTLIAFMMNKLQESISDRLLDRMNIVIMILLVFVIIYFSFKLYGLLTNTT
                     IDILYNV"
     gene            complement(2308..3066)
                     /locus_tag="PLV81_15025"
     CDS             complement(2308..3066)
                     /locus_tag="PLV81_15025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11452.1"
                     /translation="MIKTKSQSYIIVFWLFLAFSISYAQQTSHTWQQISGNWTVVHDN
                     KESYLRENRSKSFSFDYSPLINLNSLITSSDYDFTSLTHTVAINSPADGACYLTFFAA
                     SNYRQFYAVQFTGTTSSITSIQIIKSDIKDTTLPKQAKGNFIITTLATKPISLEYGRT
                     YTLSISVQDKTISVAIDNAQVLSHTLDEELESGKIGFASKNCIPVIDNLKVYKGNDIL
                     FQDDFSKESIRKIVLQGKKLTPQEVEEYKKKNKK"
     gene            complement(3253..3624)
                     /locus_tag="PLV81_15030"
     CDS             complement(3253..3624)
                     /locus_tag="PLV81_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11453.1"
                     /translation="MKKTTVLLTIISLIIGAGIGCNQDNPTEVRWYNQSTKYLNEIIW
                     SRNIGTQPNELNYDQKWTRGYDNSDRTEFKEVNLLNGYVYAIVEENGDFVLAEAIIDT
                     SNNVSLNEGNSQEYIITQISD"
BASE COUNT         1174 a          638 c          798 g         1307 t
ORIGIN      
        1 ttatcgtttg aaaacaattg cttcaacgcc tgcttcatcc atcatggctt ttgccagttc
       61 atcgttgtag ggatgttcaa atataaatgt ttttataccg gcgtttatca gcatcttggt
      121 gcatattgaa catggctgat gggtaacata aataatagca ccggctattg aaacaccgtg
      181 aaaagcagcc tggatgatgg cattttgctc cgcgtgaagg ccacggcata gctcatggcg
      241 ttcgcctgag ggtacgttgt agatgttacg caggcatgtt tctggagtgc agtgtgttat
      301 gcctgttggc acgccgttgt agccagttga cagtatgcgt ttatctttaa ctatgactgc
      361 accaactttg cgccgtatgc atgtagcacg tgtagaaaca tcatgtgcaa tcatcatgaa
      421 atattcatcc caggttggcc gtgtcatttt gcactcacct tgcattgtag ctgtaaatca
      481 actgaataat ccataaccgt atgcagcgta aatttcaccg ttaatggaat aacactgtga
      541 taaacatatc gctttggttt cagtaaatca tgtttttttg catattcttt gactggctca
      601 acaaggattg cggctgtcag cgttactggt ttttgtggtg ttatggcaag cattgtggat
      661 gaatattcaa acgattgtga taatatttcg ttattgttat gtgcgatagt tacggtaacc
      721 ggtgggtttt ttactgaaag ctgtgatgat gtgttactgg taaatgttac cgatgcaagc
      781 agtggctggt tgatggtatg aataatggaa aagcgcacct gaacaccgtt tacggcaacg
      841 gtttgataga tataatcagt tggctctttt ttctggatga tgagtgttaa gaggatgatg
      901 aagactataa tgttaatcag caaaaagatg cgtgaaaatt tctttttgcg ctgattgttg
      961 tcaggaggca tttcaccttt tttgatatat tcaatgtatt cctttgcttt ttcctgcatt
     1021 gtgttattct atctgctgta gtatagcttg aggaagagta aaatcgatgg attttagttt
     1081 gtagatatcg ccatacatca tggcaaaaaa attaattgtt aatgtcctgc tatcctgtaa
     1141 tgatttgaag aaataggcat ttacaggatt attagcaaaa tggaatgtat ggtatgtttg
     1201 aatttcctta ccatcagcaa acatggtgag tgaaattgtt ttttcatctt tttctacaag
     1261 ccatgaaaca ttggtaggca aaatatcgtc aatggcttca ggtaaatcaa aatataaaag
     1321 gtaataatca tcaggtgttg acaatagcag tgatgcgcct gcatcttcaa aaaaatcata
     1381 tagcggggca aagaactctc tgtcaagagt agagtggtca ggaagttttc gtaatgtgtc
     1441 aataaactgt tgtgttgcaa tgatgtccat attcagtaac tatcgcccaa aaaaaattta
     1501 cagtaaaaag cctgtgcaga cgttagtgta tattgacagt aactatcgcc taaacattat
     1561 acagaatgtc aatagtggtg ttggtaagta acccatacag cttgaatgaa aaatagataa
     1621 taacaaacac aagtaaaatc attatgacaa tattcattct gtcaagcaat ctgtcggaga
     1681 ttgattcctg caatttattc atcatgaatg caatcagtgt aaggtaaccg gcaagtcctg
     1741 tacagcagga taccagaaag gtggctttga ggggaatgtt catggtgtca aacagttgca
     1801 aatctaaaag aagcttatag ccaatgaccc accacacaat catgccggga ttggtaatgg
     1861 caatggttaa tcccgtaaga tatgatgtgc gttttttata ctgaatgtgg ggtttgtgtg
     1921 aaggcggtat gtgtttgtgc tcacggtatg aacggtacaa aagcgctatg atgacaagaa
     1981 taccaagtga ataggtaaag atctgaactt ttgggtaatg caaaaaacca ccaatgccaa
     2041 aaagcacaag aactccgtaa ataaaatcag aggtaaccga gcccaatgct accgatatag
     2101 ctggttttaa atggccgtta atggcttttt taatgacttc aagctggaca gcgccaacag
     2161 ggattgaaga aataaatcca ataaaaaggc caaatgcaaa taaaaaaaag atatgaatgg
     2221 tgtacatgcc ataccctaca cgtaataaaa tgcgtattat atacgatgca aattgcagta
     2281 aaaagttgca agtatttttt gtaaatatta tttcttgttt ttctttttat attcttcaac
     2341 ttcctggggt gtaagttttt tgccctgcag cactattttg cgtattgatt cttttgaaaa
     2401 atcgtcctga aacaggatgt cattgccttt gtaaactttg aggttatcaa taacagggat
     2461 acaatttttt gaagcaaagc caattttacc gctctctaat tcttcatcaa gtgtatgcga
     2521 tagtacctgt gcattgtcta ttgccacaga aatggtttta tcttgtacag aaattgatag
     2581 cgtgtatgtc cttccgtatt caagcgaaat tggttttgtg gcaagggtgg ttatgataaa
     2641 atttcccttt gcctgcttgg gaagcgtggt gtctttgatg tcagatttta tgatttgtat
     2701 ggaggtgata ctgctggttg ttccggtaaa ttgaacagcg taaaactggc ggtaattgga
     2761 tgcagcaaaa aatgtgagat agcaggcacc atcggcaggc gagttaattg caacagtgtg
     2821 tgtaagggaa gtgaaatcat agtcactgct tgtgataagc gaatttaagt taattaaagg
     2881 actatagtca aagctaaacg atttgctccg gttttcgcga aggtaggatt ctttattgtc
     2941 atgcaccaca gtccagttgc cagaaatctg ctgccaggtg tgcgaagttt gttgtgcata
     3001 ggaaattgaa aatgcaagga aaagccaaaa tactattatg tatgattgtg attttgtttt
     3061 tatcatgtta tttttgccat tgtttgcgtt ttattatgtt aattgttact aaaaaacaat
     3121 tttataaaaa aatgaattgc gaattatctt agaatttgct ttgtaaagat attataatga
     3181 tctaatgtta tcaagatttt ttaaatatat ttgtaaattt taaagatata ataaatacat
     3241 tattatatca ttttaatcac tgatttgggt tataatatat tcttgagaat ttccttcatt
     3301 aagagatacg ttattagagg tatctataat tgcttctgct aaaacaaaat caccattttc
     3361 ttcaacaatt gcataaacat aaccgtttag taaattgacc tctttgaatt ctgttctgtc
     3421 actattatca tatcctcttg tccatttttg atcataattt aattcatttg gttgtgttcc
     3481 aatatttcta ctccatatta tttcatttaa gtacttagtt gactgattat accatcttac
     3541 ctctgttggg ttatcctgat tacacccaat gcctgcacct ataatcaggc ttataattgt
     3601 cagcagtact gtggtttttt tcataatgat cctccgtagt atatatgcta tactctgtaa
     3661 attgtcttat agaagttacg ttcatcaaaa aacacaaagc attgtgtctt tacatcctat
     3721 gcaccacgca tgcagtgaga cgtcatgaat gccgtgagat gccaggcatg gcgtctttac
     3781 agggtagatg tggtagggca tcttccctgt aaagtcgtca cacataacgt ctctgtgtaa
     3841 aagatagtat tattgtatac cagtgtcaat taattttgtg caaatgtttt ttaatttaaa
     3901 ggctctgacc cctattt
//
LOCUS       DAORYL010000152         3842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_15871_length_3842_cov_12.327700, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000152 DAORYL010000000
VERSION     DAORYL010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15871_length_3842_cov_12.327700"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..2638)
                     /locus_tag="PLV81_15035"
     CDS             complement(95..2638)
                     /locus_tag="PLV81_15035"
                     /inference="COORDINATES: protein motif:HMM:NF021477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2194 domain-containing protein"
                     /protein_id="HOM11454.1"
                     /translation="MKSSFIKTIALVAVSFYIVIFCTVSSFAKAKGLTVLCIYKSSEG
                     YTDDSNPLKWFFEKDITSNGLRVHYHDFDKGFSSLSNLEDIRAIVTWYNSGVVASKDI
                     GINYAKFMIDAADKGIKIIIANSYGAYGYKDGNETKWDLLPYIRPLFTKIGIYFQGFW
                     TNNPNNIKIIYKDSTMVEKDEKQDVTKSLHYQQIIPLREDVKTYLQLQRTDAPPQAGD
                     GKSSVIVISKTGAFALENYVVRGSKVMLNTSAFIQEALFYDDGYLNVGVMIGDMDRAN
                     VLLSNISYAFKYAKIRYDIYTKNELKKLVAKDLSEYEAIVIATKTKEAIPYELLKGYV
                     ENGGKCIFLNYVEPDNELNKFIGIKEYTKKEEYFKQGFTLHPSLFLNGVNVPGDDIDC
                     NVRRAVLENDVKVLGTVNTLFDADKYPVVWERTIKKGKILYWNTDLLDESKLTRGMIV
                     QSIYKVADVFATGMINVGLMMIDDFPAPWWNVAYKPYRIKYYSDLLQNEKDKFNRKKL
                     EEIIEKLKKLPEETDTLFISDVWFKDILAWQKQFGFVYSSFLIFNYNMNTRADETGFS
                     VRDFYLSQNGLSTKMGIAALENGFELGLHGYNHMSLTLTKPAEYDSVPWPDKKTMIQA
                     LTVAKNEWIALYGESALPFSYVAPHNIIDATGLQALGEVFPSIRVVSTLYVAKSGEVE
                     QEFDWTPDNRFYQIPRITSGYYFQDFDKFALYDAFHNFGVISHFIHPDDVFDEMRSKS
                     YEGWLWLKSNFEKEFGLLKQNYPWIRWMTVKDAFYEFIVYNSSAVNFKVRGNSIEIYT
                     PGGAGHSYYIRARIPKQHKKLINCKLVQYTPSTGDIVVKTNKYKSIIVW"
     gene            complement(2619..>3842)
                     /locus_tag="PLV81_15040"
     CDS             complement(2619..>3842)
                     /locus_tag="PLV81_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11455.1"
                     /translation="FATLSLTAFDEKGIQQWWIEIYNEKGEKIQEYSLTGAVPGTFRW
                     DGTARNNVLAPEGKYYVQLTIQNSAGERFSSKKQEVVLSTAKRAVALTPSYPKFSPND
                     DKIKDTVSFIIQATDKKNVANWFVSISSKNKTVKTIRGNDFIPYDVLWDGLDDNLKTV
                     PDGEYSVALTIKYDDGIVVKSPATTVTVVTRVAVDIKVDPNEIIPLQTPLRITPSVDG
                     DVSQWQIRFMRDDGAVVKVINGAGIPPKVISWDGTDDAGNPAVYNMQLYTQMEVTDDA
                     GNTAVSKKVPFMVGFLVKQDKGKRVIYMFNQDTIFKGRSDELTVNGVLILDQLAGKLA
                     KMGGFTRINIVAYTDAVGSKQSNMSLSQKQAAVIADRLKNLAAEIKTVGAGAEILYLK
                     DKPSKWDMRYEIELY"
BASE COUNT         1142 a          811 c          594 g         1295 t
ORIGIN      
        1 gatatgagga gtattaccga aagaagtatt ccatgatact tgggtgggtg taaaagaaaa
       61 aaggggtcag agctctatca gcccttatta gtaatcacca tactattatt gacttatact
      121 tattagtttt aacaacaata tccccggttg acggtgtata ttgaaccagc ttgcaattaa
      181 taagcttttt atgctgtttg gggattcgtg cccgtatgta ataactgtgc cctgctccac
      241 cgggagtata tatttcaata ctattgcccc taactttgaa attaacagca gaagaattat
      301 atacaatgaa ctcatagaaa gcgtccttca ccgtcatcca gcgtatccag gggtaatttt
      361 gctttaataa accaaattct ttttcaaagt tgcttttcag ccacaaccac ccttcataag
      421 attttgagcg catttcatca aagacatcat caggatggat aaagtggctg ataactccaa
      481 agttatgaaa agcatcatag agcgcaaatt tatcaaaatc ctgaaaataa tatcctgatg
      541 taatacgtgg tatctgataa aatctgttat caggtgtcca gtcgaactcc tgttcaactt
      601 caccgctttt tgcaacatac aatgttgata caactcgaat actggggaaa acttccccca
      661 aagcttgcag tcctgttgca tcaattatgt tatgtggtgc tacatagcta aaaggtaaag
      721 cactttctcc atataatgca atccattcat tttttgctac tgttaaagcc tgtatcattg
      781 ttttcttatc aggccatggt accgaatcat actccgctgg ttttgttaat gttaatgaca
      841 tgtgattata cccatgtagt cccagctcaa aaccattctc aagcgcagct atacccattt
      901 tagtgctcaa accattttgt gaaaggtaaa aatcccttac tgaaaatcca gtttcatcgg
      961 ctctggtatt catgttataa ttgaatatta aaaaacttga ataaacaaaa ccaaattgct
     1021 tttgccatgc aagtatgtct ttaaaccaaa catcagaaat aaatagggtg tctgtttctt
     1081 caggtagttt ctttaatttt tcaataattt cttcaagctt cttgcggttg aatttgtcct
     1141 tttcattttg taacaggtca ctgtaatatt tgatcctgta tggcttatat gcaacattcc
     1201 accatggtgc agggaaatca tctatcatca ttaaaccaac attaatcatt cccgtagcaa
     1261 aaacatcagc aaccttataa atgctttgaa caatcattcc gcgagtcaac ttggattcat
     1321 ccagcaaatc agtattccag tacagtattt tgcctttctt tattgttcgt tcccaaacta
     1381 caggatattt atctgcatca aataatgtat taaccgttcc taaaactttg acatcatttt
     1441 ccagaactgc cctgcgcaca ttacagtcta tatcatcacc aggtacattt accccattta
     1501 aaaacaatga gggatgtaac gtaaaaccct gtttaaaata ttcttctttt ttagtatatt
     1561 ctttaattcc aataaactta ttcaattcat tatctggttc aacataattt aaaaatatac
     1621 atttcccacc gttttcaaca tagcctttta ataactcata agggatggct tcttttgttt
     1681 tagtagctat cactatagct tcatattcag ataaatcctt tgcaaccagc tttttaagtt
     1741 catttttagt atatatatca tagcgtattt tggcatattt gaaagcatac gatatattac
     1801 taagtagtac atttgctcta tccatatcac caatcatgac gccaacatta agataaccat
     1861 catcatagaa cagagcttcc tgtataaaag ctgaggtgtt gagcattact ttgcttccac
     1921 gcacaacata attttccaga gcaaacgccc ccgtttttga tataacaatt acacttgatt
     1981 tgccatcgcc agcctgaggt ggtgcatcag tgcgttgaag ctgcagatac gttttgacat
     2041 cttcacgtaa tgggataatc tgctgatagt gcaatgattt tgttacatcc tgcttttcat
     2101 ctttttctac cattgtactg tctttatata taatctttat gttgttagga ttgttagtcc
     2161 agaaaccctg gaagtaaata cctatttttg taaatagtgg gcgtatatag ggtagtaaat
     2221 cccattttgt ttcattgcca tccttatagc catacgcacc atatgaattg gcaattataa
     2281 tttttatccc tttatctgct gcatcaatca tgaactttgc atagtttatg cctatgtcct
     2341 tacttgctac aactccgcta ttataccagg taactatcgc cctgatatct tcaagattac
     2401 ttaatgatga aaaaccttta tcaaaatcat gatagtgaac tctcaatccg tttgatgtta
     2461 tatccttttc aaaaaaccac tttaaaggat tactatcatc tgtatatcct tctgacgatt
     2521 tatagataca caacacggtg agtccctttg cttttgcaaa agaactcact gtgcaaaata
     2581 tcacaatata gaaagatact gctactaaag cgatagtttt aataaagctc gatttcatag
     2641 cgcatatccc attttgaagg tttatcttta aggtacagta tttctgcacc tgcaccaaca
     2701 gtttttattt ccgctgcaag attttttagt ctgtcagcta ttactgcagc ctgtttttgc
     2761 gataaagaca tattggattg tttgctccca acagcatcgg tataagcaac tatgttaatc
     2821 cgtgtaaagc cacccatttt tgccagttta cctgcaagtt ggtcaagtat caaaactcca
     2881 ttgactgtga gttcatcact tcgcccttta aatattgtat cctggttaaa catatatata
     2941 acccgtttac ctttatcctg ctttaccaga aaacctacca taaaaggaac tttttttgat
     3001 actgcagtat tgccggcgtc atcagttact tccatctgcg tatagagttg catattgtaa
     3061 acggctggat tgcctgcatc atctgttcca tcccatgaaa taactttagg cggtatcccg
     3121 gctccattaa ttaccttaac cacagcacca tcatcgcgca taaatcgaat ctgccactgg
     3181 gaaacatcac catcaactga cggagtaatg cgcaatggtg tctgaagcgg tataatttca
     3241 tttgggtcca cttttatatc aacagctact cgtgtaacca cggtaacagt ggtagcaggc
     3301 gacttaacga caatcccatc atcatatttt atggttagtg caacggaata ttcaccatca
     3361 ggtactgttt ttagattatc atccaatcca tcccacaata catcatatgg gataaagtca
     3421 ttacctctaa ttgttttaac agttttattc ttactgctta tagaaacaaa ccagtttgca
     3481 acattcttct tatccgttgc ctgtataata aaacttactg tatctttaat cttatcatca
     3541 tttggtgaaa attttggata tgatggggtc agagcaacag ctcgttttgc tgtagaaagt
     3601 acaacctcct gctttttaga actgaatcgc tcgcccgcag aattctgtat agtaagttga
     3661 acatagtatt tgccttcagg agccagcaca ttgtttctgg ctgtaccatc ccatctgaat
     3721 gttcccggca ccgcaccagt taaactgtat tcctgaattt tttcgccctt ttcattataa
     3781 atttcaatcc accactgctg tatacctttt tcatcaaatg cagttaaact caacgttgca
     3841 aa
//
LOCUS       DAORYL010000153         3838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_15892_length_3838_cov_6.659794, whole genome shotgun sequence.
ACCESSION   DAORYL010000153 DAORYL010000000
VERSION     DAORYL010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15892_length_3838_cov_6.659794"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3838)
                     /locus_tag="PLV81_15045"
     CDS             complement(<1..>3838)
                     /locus_tag="PLV81_15045"
                     /inference="COORDINATES: protein motif:HMM:NF023677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin TcdB middle/N-terminal domain-containing
                     protein"
                     /protein_id="HOM11456.1"
                     /translation="TTGAFDKSLLQRVEVRSGSGQLFYAHDFEYYDEVGWGNSQLNLF
                     DAPVVMENTEEIKTILIGPFVIGSIGGSTSSGLQVQGSAGFSPFNWVNALSIGPDGSA
                     AENTTHTQIQLIDIDGDGMPDQVYKEGEFDVKYRRNKGDNQFDNEVEIPGLCGLGKDE
                     SRTITKGVGANVYMVGKKRQWSNTNAFTKEYFTDVNGDGYVDFINGSTVYFNHSYTGT
                     VYFSTTPPAGFVVVQEEVNVPVNPIDEGQEDEGKYDFMDMFYRDNPVIAWRAPFSGRI
                     AIEGDVSFVQEINSYIQQLGYTGADGVRVTIEKPGDTQPLWSSVIDRNGSVTPSINEL
                     WVNAGDYILFRVDSKRDGAYDEIIWNPQITYKGYVNEAVDENNMYIYQYKLADDFYVN
                     GGGIFEPPFAGVGTIKGGRIYKYAKTSDNISYKIIKRDVTQTILIGDNQSNPGEQVYS
                     PAVEILSGEIEAGYVGQVNIGNYSNILITKHEEKTYDGDKISSITVTKTILECRMDAD
                     TPIDWRQVQWMNDDGKQGPQVQYTDIVLPEGQQMNENDMQSLLNSVYRIPVNTKLFTV
                     PLTAGNGTGKIDPYVFEGWNIPREGNYRVVITAIYNGDNTYNEDLTLCVKKRISGGGF
                     IADVVGKRGLDKEIGLEKNQQLELDTIENDILECNEGDELYFVITATKPEWFSFDVTV
                     YYDICSTNPWVDDRIWVEIPVEYCIYKSVDPGKDIIFAGGFRNWYYGRWNADEFVDEE
                     VAGNPDYIYDYELKDIKQSYLKVDYEEVQNDPEGFAEKIKLCSPMIPKFFDTLGDGEQ
                     QIKDRQRTTELVLPCWQGMDADSWIGLKVENGKKNVIIATTRLIKKNIEDLLHPEQEV
                     GNQSTTQSVTITQIQNAGTVDRRGVSKYTRSYSKMDSLSAGLSYATSKTKSITKRQFF
                     DINGDRYPDIVTDAGAVVTNIMGGLYKSLSFLQGAIQYTEGKNTNWSINPQTITTMAV
                     LCIRGNGILEKVATAMPSMTLTRATSSSKVLKDLVDINGDGLPDKIECNDSGCVVLYN
                     TGNGVVTGQVLPITAIQKSELQSISASDGTGFSIDKSFYSGGISISDTSNDTGYILMD
                     INGDGMPDKIHDNGKIYFYNGQGFATIYKQTDAGLDRTRGQSVNLSATAGGKLPVFIP
                     IPFALIFIGTIEISGGGGGTMDGNATRYTLRDINGDGLPDIVYQSGDPLQSNSLYVRL
                     NRTGKTNMLKSIKNSIGGTIELDYKRVGNTRLMPQSQWVLATVTTDDGTSTIDPTPGS
                     VHRYTTNYDYADGVQDR"
BASE COUNT         1060 a          906 c          554 g         1318 t
ORIGIN      
        1 cgatcctgca caccatctgc atagtcatag tttgtggtgt agcggtgtac actgcctggt
       61 gttgggtcaa tggtggatgt accatcatca gttgtaacgg tggcaagtac ccactgactt
      121 tgcggcataa gacgggtatt gcccacacgt ttgtaatcaa gctctattgt accgccaatg
      181 gaatttttaa tggatttaag catgttggtc ttgccggtac ggtttaaacg cacataaagt
      241 gaattagatt gtaatggatc tccactttga tatacaatat cgggcaaacc atcaccattg
      301 atgtcacgca aagtataacg ggtggcatta ccatccattg ttcctccacc acctccgcta
      361 atttcaattg taccaataaa aattaatgca aatgggatcg gtataaatac aggaagcttc
      421 ccacctgcag tagctgacaa atttactgat tgccctctgg ttctatcaag accagcatca
      481 gtttgtttat atatagtagc aaaaccttga ccattataaa aatatatttt cccattatca
      541 tgtattttat caggcattcc atcgccattg atatccatta atatgtatcc tgtatcatta
      601 cttgtatcgc ttattgaaat gcctccacta taaaatgatt tatctatact aaacccggtt
      661 ccatcactgg cagaaatact ttgcaattct gatttttgta ttgccgtaat tggtaatact
      721 tgacctgtaa caacaccatt ccccgtgtta taaagtacca cacagccact atcattacat
      781 tcaattttat cagggagtcc atcgccattg atatcaacta ggtctttcaa tactttgcta
      841 ctactggtag ccctggttaa tgtcatgctg ggcatggcag tagcaacctt ttccagaata
      901 ccattaccac gtatacacaa taccgccata gttgtgattg tttggggatt tatactccag
      961 tttgtgtttt taccttcagt atattgtatt gctccctgga gaaatgataa cgatttatac
     1021 aatccaccca ttatatttgt tacaacagcc cctgcatctg taacaatatc tggataccta
     1081 tcgccattta tatcaaaaaa ttgccttttg gtaatacttt ttgttttact tgttgcatat
     1141 gacagtcctg ctgataatga atccatttta ctatagcttc ttgtatactt tgacactccc
     1201 cgtctatcca cagtaccagc attttgtatt tgtgttatag taacactttg cgtggtactt
     1261 tgattaccta cttcctgttc aggatgcaac agatcttcaa tattcttctt gataagccgc
     1321 gttgttgcta ttattacatt tttcttccca ttttcaacct ttaacccaat ccagctatca
     1381 gcatccatcc cctgccagca gggcaaaaca agctcggttg tacgttgtct atctttaatc
     1441 tgttgttcac catcgcctaa tgtatcaaaa aattttggta tcatgggtga gcacagtttg
     1501 attttttcag caaaaccttc tggatcattt tgaacttctt cataatccac ttttaaataa
     1561 ctttgcttta tatcttttaa ttcataatcg taaatataat ctggatttcc tgcaacttct
     1621 tcatccacaa actcatctgc attccaccta ccatagtacc agtttctaaa acctcccgca
     1681 aatataatat ctttaccggg atcaacactt ttataaatac agtattcaac tggtatttcc
     1741 acccaaattc tatcatccac ccaaggattt gttgagcaaa tatcataata aacggttaca
     1801 tcaaaagaga accactcagg ttttgttgct gttatgacaa aatagagctc atccccctca
     1861 ttgcattcca gtatgtcatt ctcaatggta tcaagttcca attgttgatt tttttcaagc
     1921 cctatttctt tatcaagacc ccttttacca accacatcag caataaatcc tccacctgat
     1981 attctttttt tcacgcacag tgtcaaatct tcgttgtatg tattatctcc attatatata
     2041 gccgttatga caactctgta atttccttca cgtggtatat tccagccttc aaatacatac
     2101 ggatcgattt tcccggtacc attaccagcc gttagtggaa ccgtaaataa ctttgtattt
     2161 acggggatcc tgtaaactga attgagcaat gactgcatat cattctcatt catttgctgt
     2221 ccttcaggta acactatatc agtatattga acctgtggcc cctgtttgcc atcatcgttc
     2281 atccattgta cctgtcgcca gtcaattggt gtatctgcat ccatcctgca ttcaagaatt
     2341 gtcttcgtca ccgttatact tgatatttta tctccatcat aggttttttc ttcatgcttt
     2401 gttattaata tattgctgta attaccaata tttacctgac caacataccc tgcttctatt
     2461 tctccactta gtatttcaac tgctggagaa tatacttgtt cgccagggtt actttgatta
     2521 tcacctatta aaattgtttg tgtaacatct cgttttatta tcttatacga tatattatcc
     2581 gaagtttttg catacttata tattctccca cctttaatgg ttcctacacc tgcaaacggt
     2641 ggctcaaata tcccaccacc attaacataa aaatcatctg ccaatttata ttgatagata
     2701 tacatattgt tttcatcaac cgcttcattt acatatcctt tatatgttat ttgtggattc
     2761 cagattatct catcatacgc tccatctctt ttactatcta ctctgaaaag gatataatca
     2821 ccagcattaa cccacagttc gtttatagat ggagttacac tgccattcct atctatcact
     2881 gatgaccaaa gaggttgcgt atcacctggc ttttctatgg taactcgtac tccatcagca
     2941 ccggtatatc caagctgttg aatatatgaa tttatttcct gtacaaatga aacatcgcct
     3001 tcaattgcta ttctaccgct aaatggcgca cgccaggcta ttactggatt atcccgataa
     3061 aacatatcca taaaatcata ttttccctca tcttcctgac cttcatctat agggttcacc
     3121 ggcacattta cctcttcctg taccactaca aagcctgcag gcggtgtggt ggaaaaatac
     3181 acagttccgg tatatgaatg gttaaaatag accgtgcttc cattgataaa atcaacatat
     3241 ccatcgccat taacatcagt aaaatattct tttgtaaacg cattagtatt actccattgc
     3301 ctcttcttgc caaccatata cacattagca ccaactcctt tagtaattgt cctgctttca
     3361 tctttcccca atccacataa acctggtatt tccacttcat tatcaaactg gttatcccct
     3421 ttatttcttc tatactttac atcaaattca ccttctttgt atacctgatc aggcatccca
     3481 tcgccatcaa tatcaattag ttgtatctgg gtatgagtag tattctctgc tgcacttcca
     3541 tcaggaccta ttgataatgc atttacccag ttaaagggtg aaaaaccagc cgaaccctgt
     3601 acttgtaatc ctgaacttgt actgccaccg attgatccaa ttacaaaagg tcctattaaa
     3661 attgttttta tttcctctgt attctccatc actaccggtg catcgaacag gttcaactgg
     3721 ctattccccc agcctacttc atcataatac tcaaaatcat gcgcatagaa aagctggcca
     3781 ctacctgaac gtacttccac ccgctggagt aaactcttat caaatgctcc cgttgtat
//
LOCUS       DAORYL010000154         3818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_15993_length_3818_cov_12.036939, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000154 DAORYL010000000
VERSION     DAORYL010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15993_length_3818_cov_12.036939"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1944)
                     /locus_tag="PLV81_15050"
     CDS             complement(<1..>1944)
                     /locus_tag="PLV81_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11457.1"
                     /translation="TNRNISEDTGYTFDNPFTTVVGSGPALSSTTIGDGVLNTEDLND
                     NGMLDTGEQYIRIPGLTTPITIDCANTTWQKVRIYINKNNPALYTSSPDAFEDILKKI
                     YAIRLILQKNSATTGTILIDTIRFVTMHWQVDSIDGISGNNTDKVKLTLIDSFNDSEY
                     ALESFARSKSDVYTSLYGDKTKKELTQTMESALNVTYTNITAATIKRKFYKPMDLRFY
                     RNVHCWINVRNYTPGDTLIFRLHSSDNDYIEYSYNPQFIQTWEDIVLSLQYQDTNAHF
                     IQKEGNPDLKRIIAITVSVQNTTTSGQFWLDDIYASDPLTLEDTACWYEGTMKITKPV
                     ARTQAGTPVLSDITLSYLKKQHGNNFYTIGQPYNDISEDYNQVTVTCQVLPYWHTSLD
                     FIQEESQTDSLNEQVSATRKGITSIKQFHFASTLSPPNNTIPQLDVLYNYENFNNKQA
                     YYQDVNTFDNDTSKITHQATVGVQQLLHDVLGGDLSYRLLLDTSFKEDIFKENSQSVA
                     TGLNTQKKQRESCSMNINYQLTHFFISPTIQILSEEFITYSGTVTDINPALSQEISSE
                     YHIPFVYGDSIRFIERLKKSSLSFGLKSYKLINPSITYEFSYFENQFKDLQPYDIWMT
                     LGFNRTRSTQGFLSSTIAIPINLQ"
     assembly_gap    1945..2044
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2045..>3818)
                     /locus_tag="PLV81_15055"
     CDS             complement(<2045..>3818)
                     /locus_tag="PLV81_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11458.1"
                     /translation="YIDHDSQQRTNTYSMQYKAVEEDEVLREINAGDITIKVNNSKYA
                     VYDDTTSKGMGIDFTIKKGNLQVKAFGSVAKGVSEVERFKGNYSSTSITLQEYQYTKA
                     TYYQLEPFKRYDNLDSPPSNMYTTITWTSAPVNPSTFTPYSVGIDSSGFELYADDAIT
                     QNKSGLLELHRWDNPSIILGYFRKLQQGSDYTINFSTGTIALLKAFKPQAKLFAVYTR
                     NGGTTQTSDPSAITNHPQFPGKICVFIKYGPLLHEDVNYNGTLESGEDTNNDGVLNID
                     IYEIRGIYTTGLQNIRAEDVKISFFSNLFPMSRSDIQKLGNFIIDYNTGAIIFKLREP
                     FKQLLPTQSQKIIYNEAQTQAYTVSQYKIGIAGYSQSRGFQLKHVNIIPDTIRVKING
                     IQIAESLYSVDPTSGFFQFTNPGNPVITPETDIEISYQYLPFEGQAQQFIGGMRADYA
                     LNKNINVGGTILYTTQGIASTVPLLGNEPTQTTVVEADSSVEVSPHDISAFASKIAQS
                     DISVPVLFKGYAEYAKSYHSINTFGKGLIDDFESIYSGLTVSMSEKDWVLGSLPDSVP
                     QNLRARIYYKFYRDINNPETLYGL"
BASE COUNT         1037 a          637 c          789 g         1255 t
ORIGIN      
        1 ttggagattt atgggtatag caattgtact ggatagaaat ccctgtgtag aacgggtgcg
       61 attgaatcca agggtcatcc atatatcata gggttgcagg tctttaaatt gattttcaaa
      121 gtatgaaaat tcataggtta tagaagggtt tattagttta tagcttttta gaccaaagga
      181 aagagaactt tttttcagac gctcaataaa tcgaatacta tcaccatata caaaaggaat
      241 gtgatattct gaactaatct cctgtgataa tgctgggttt atgtcagtta ctgttcctga
      301 gtaggtgata aattcttctg atagaatctg tatggtgggt gaaataaaaa aatgagttaa
      361 ttgataatta atattcattg aacaggattc acgttgtttt ttttgtgtgt ttaatcctgt
      421 tgctactgat tggctgtttt ctttaaatat atcttcttta aaagaagtat caaggagcaa
      481 cctgtacgat aaatccccgc ccaatacgtc atgtaacaat tgctgcacgc caactgttgc
      541 ctgatgcgtt attttgctgg tgtcattatc aaacgtgttc acatcttgat aatatgcctg
      601 tttgttgttg aagttttcat aattatataa tacgtcaagc tgggggatcg tattattggg
      661 aggggagagg gttgaggcaa aatggaattg ttttatactg gttattcctt ttctggttgc
      721 ggatacctgc tcattaagac tatcagtttg tgattcttcc tgaatgaaat caagtgatgt
      781 gtgccagtac ggaagcacct ggcaggttac tgttacctgg ttatagtctt cagaaatatc
      841 attatatggt tgccctatcg tataaaaatt attcccatgc tgttttttta aatacgatag
      901 agtaatatcg gatagcactg gagtccctgc ctgggtgcga gcaactggtt tggtaatttt
      961 catagtacct tcataccagc aagcggtatc ttcaagtgtt agggggtcac ttgcatagat
     1021 atcatcaagc cagaattggc cactggtagt ggtattctgt actgaaactg tgatagctat
     1081 gattcgttta agatcaggat taccttcttt ttgtataaag tgtgcatttg tatcctggta
     1141 ttgcaatgat agtacaatat cttcccatgt ctggataaac tgtggattgt atgaatattc
     1201 aatatagtcg ttatctgaag aatgcagacg gaaaataagc gtgtcaccgg gagtgtaatt
     1261 tctgacatta atccagcaat ggacattacg gtaaaacctg agatccattg gtttataaaa
     1321 ttttcttttt atagtggctg cagtaatgtt agtataagtg acattcaggg cagattccat
     1381 tgtctgggtg agttcttttt tagttttatc gccatacagg ctggtataaa catcactttt
     1441 actacgtgca aatgactcca gtgcatattc tgaatcatta aatgagtcaa tcagagtgag
     1501 cttcacttta tcagtattgt tgccacttat gccatcaata ctatccacct gccagtgcat
     1561 tgtaacaaag cggatggtat cgataaggat tgtgccagtt gttgctgaat ttttttgtaa
     1621 tataagtcta atagcatata ttttttttaa tatatcctca aaagcatctg gcgatgaggt
     1681 atacaaagcc gggttatttt tatttatata tatccggact ttttgccatg tggtattggc
     1741 acaatctatg gtgattgggg ttgttaatcc aggtatgcgt atatactgtt cgccggtatc
     1801 aagcatgcca ttgtcattta aatcttcggt gttgagcact ccatcgccaa tggtggtaga
     1861 tgatagagcc gggcctgagc ctacaactgt tgtaaatgga ttgtcaaacg tatatcctgt
     1921 atcttcagaa atatttctat tggtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnntttaat ccatagagtg tttcagggtt attaatatca cgataaaatt tataatatat
     2101 tcgggctcgt agattctgtg gcacactgtc aggcagcgaa cccagtaccc agtctttttc
     2161 ggacattgat actgtaagcc ccgagtaaat tgattcaaaa tcatcaatta atcctttgcc
     2221 aaaggtatta atgctgtgat aactttttgc atactctgca tagcctttga acagtaccgg
     2281 tacggaaata tcggactgtg ctatttttga ggcaaatgct gatatatcat gagggctcac
     2341 ttcaacggaa ctatcggcct ctacaacagt tgtttgtgtt ggttcattgc ctaacagggg
     2401 aacagtggat gctataccct gagtggtata cagaattgtt ccaccaacat taatattttt
     2461 attcagagca taatcagccc gcatgcctcc aataaattgc tgagcctgtc cttcaaaggg
     2521 taggtactgg tagcttattt caatatcggt ttcaggagtg attacgggat tccctggatt
     2581 ggtgaactgg aaaaaacccg atgtgggatc aacgctgtac aggctttcag caatctgaat
     2641 gccattaatc ttaactcgta tggtgtcagg gatgatattg acatgtttca actgaaagcc
     2701 tcgtgactgg ctgtaacctg caatgccaat tttatactgg gatacggtat acgcctgtgt
     2761 ttgtgcttca ttgtagataa ttttttggga ttgtgtgggc aaaagctgtt taaatggttc
     2821 acgaagctta aatataattg cgccagtgtt ataatcaata atgaaatttc ctaatttctg
     2881 tatgtcggat ctggacatgg ggaaaagatt gctaaaaaac gatatcttaa catcttcagc
     2941 gcgaatgttt tgtaatcccg tagtatatat cccacgaatt tcgtatatat caatatttaa
     3001 tacgccatca ttgtttgtat cttcccctga ctcaagagtg ccgttataat tgacatcttc
     3061 atggagaagt ggaccatatt ttataaatac acatatttta ccagggaatt gtggatgatt
     3121 ggtaatggca gaagggtcgg aagtttgtgt agttccacca ttgcgggtat acacggcaaa
     3181 gagctttgcc tggggtttaa aagctttaag cagggcgata gttcctgtgg aaaagtttat
     3241 ggtataatca gaaccctgct gtaattttct gaaatagccc aaaataatag aaggattatc
     3301 ccatctgtga agttccagca atccgctttt attctgtgtg atagcatcat cggcgtacag
     3361 ttcaaaacca gaactatcga taccaacaga atagggtgtg aaggttgatg gattaacggg
     3421 tgctgatgtc catgtaatgg ttgtatacat gtttgatggg ggagaatcta aattgtcata
     3481 gcgtttgaac ggttccagct gataataggt ggcttttgtg tactgatatt cctgcaatgt
     3541 aatgcttgta ctggaatagt tgcctttaaa acgttctact tcactgacgc cttttgcgac
     3601 cgaaccaaac gcttttacct gtaaattgcc ctttttaatg gtaaaatcta tacccatgcc
     3661 tttggatgtg gtgtcgtcat atactgcata ttttgaatta ttaactttaa tggttatatc
     3721 accagcatta atttcacgta atacttcatc ctcctcaaca gccttgtatt gcatactata
     3781 ggtattggtt cgctgctggc tatcatggtc aatgtata
//
LOCUS       DAORYL010000155         3799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_16125_length_3799_cov_14.138355, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000155 DAORYL010000000
VERSION     DAORYL010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16125_length_3799_cov_14.138355"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1351)
                     /locus_tag="PLV81_15060"
     CDS             complement(<1..1351)
                     /locus_tag="PLV81_15060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOM11459.1"
                     /translation="MKTTSDSLSIKTKIGYGVGDFGANMVFQSVVLYLMFYLTDVFLI
                     PASVAGTIFLIAKIWDAVSDPMMGYISDKTRSRWGQKRPYLLFGAIPLGLSMALLFYT
                     PSLGDEAKILYALVMFLLVCTAYTVVNIPYGALTANLTLDSTERSRLTGYRMFCAVVG
                     TLLVAGATKPLAGLFQNPVEGWRFVGVLYGAIAAVFTLITFASVRERVQHSETEEYRL
                     KDIATTLSVNKPFIFLSIGVFMHLCAIGVLASMVNYLFKYNFNKEDFIPIAFLSMFAT
                     AAVAIPLWVFISNKIGKKHAFNIGMGLLAVILVVLYFAKQFNFVFFTVLFVAAGVSVS
                     TVFFSPWAMIPDTVEYSQWKTGLRREGIIYGFFYFSQKLAAACAGFITGIGLTLCGYI
                     QPKLINNILTAQIQSCDTLTGLKILTTLIPLALIICGIVFISLYPIDEKMHKDIVQKI
                     "
     gene            complement(1608..3110)
                     /locus_tag="PLV81_15065"
     CDS             complement(1608..3110)
                     /locus_tag="PLV81_15065"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOM11460.1"
                     /translation="MKHINVAFIGLGRIADLHYLGYKNNKHAKLYAVCDINQKLVEQR
                     AKQWHATAAYTDYNDLLKDPHIDAVEILTPHSLHEPVVIAAAKAGKHIALQKPMTVDL
                     ASADRILAATRESGKIFKVTDNYAFYPPIRLAKKIIENGEIGNPIAIRIKFIGGGSGG
                     WYVPPEAWQWRLKENTESGGVRGFDTFDHGHHLWTTAWYLCGNVERASGWIDSIDGII
                     DAPSVMIWKHKNGVYGSIEFVHMPALKIPSKYYANDEWIEVSGTQGMVIIHRCTGNIV
                     KGPAVSVFTTKGWKHYNEKSDWALGFIGATHNFIESILGKAQPVLSGADARDILRFSL
                     AIQKSAKVHREVYTEELDASFPSLYYYLRHRKDIAAAKPKKSFLERIGLSGNTSHYAP
                     QAKELTDEFLKRYNPDAVKQWTVTIALNIEPDGETKGFCYSIMINNSVLTAKEGELPQ
                     KWDMKITVPAGLWAAILLKKKRIEMAYLQGRIKIEGKSEEALKLRSAFGI"
     gene            3513..>3799
                     /locus_tag="PLV81_15070"
     CDS             3513..>3799
                     /locus_tag="PLV81_15070"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOM11461.1"
                     /translation="MVTEGKYVAIHYTGKFDDGQVFDSSLDGAPFEFQAGAGMVIPGL
                     DRAVLGMKLDEEKDIVIAPEDGYGDYDENLLYAFPLEEVQQQFKPEIGMTIG"
BASE COUNT         1119 a          862 c          696 g         1122 t
ORIGIN      
        1 aaatcttttg cacaatgtcc ttatgcatct tttcatctat gggataaaga cttataaaaa
       61 caatcccgca tataattagt gccagtggta tcagtgttgt aaggattttg agtcctgtaa
      121 gtgtatcaca actttgaatc tgtgcagtta atatattgtt tattaacttt ggctgtatat
      181 atccacacag cgtaagccca ataccggtaa taaatcctgc acatgctgct gctaatttct
      241 ggctgaaata aaaaaagcca tagataatcc cttctcttcg caagcccgtc ttccactgtg
      301 aatactcaac ggtatcagga atcatagccc acggactaaa aaatactgtg gaaacgctca
      361 ctcctgctgc aacaaaaagc acagtaaaaa aaacaaagtt aaactgcttt gcaaaataca
      421 gaacaaccag aataacggca agcaaaccca tgccaatatt gaatgcatgt tttttaccaa
      481 ttttatttga aatgaaaacc cataagggga tagcaacggc agcagtagca aacatggata
      541 aaaatgcaat tgggataaaa tcttctttgt taaagttata tttgaagaga tagttaacca
      601 tagaagccag cacccctata gcacacagat gcatgaatac accaattgaa agaaagataa
      661 acggtttatt cactgatagc gttgttgcaa tatccttcaa tcggtattct tctgtttcac
      721 tgtgctgtac acgctcccgt actgatgcaa atgttatcaa ggtaaaaacg gcagcaatag
      781 ctccgtacaa aactccaaca aaacgccagc cctcaaccgg attttgaaaa agccctgcaa
      841 gcggcttagt tgctccagct accaataatg ttcccaccac agcacagaac atcctgtaac
      901 ctgtaaggcg tgagcgctcg gtgctgtcaa gagtaaggtt tgcggtcaat gcgccgtatg
      961 gaatgttgac aaccgtgtat gcagtgcata caagcaaaaa catcaccagc gcatacaata
     1021 tttttgcttc atccccaagt gaaggcgtgt aaaacagtaa cgccatggaa agccccagcg
     1081 ggattgcacc aaaaagcagg tacggccgtt tttgtcccca cctgcttcgt gttttatcgg
     1141 aaatatatcc catcatgggg tcactcactg catcccatat ctttgcaatt aaaaatatag
     1201 taccggccac tgaagctggt attaaaaaca catcggttaa ataaaacatg agatacaaaa
     1261 caaccgactg aaataccata ttagcgccaa aatctcccac tccatatcct atctttgttt
     1321 ttatggataa gctatctgat gttgttttca tgtctcctct cctccaaaaa taaaagttac
     1381 cccttactgc aatcctgaag tcaagtagtg gctgaaggat ctcattgcct tcagcataag
     1441 attcttcgtc ccgattgcat cgggactcag aatgacatgg tgattgtcat cctgaagccc
     1501 tacattcccc tctgtcatcc tgaagccacg cagtggctga aggatcttgc cgtttcaaac
     1561 acaagattct ttacccctat tgcatcggaa ctcagaatga ctttcaacta tatcccaaag
     1621 gcagaccgca atttcaacgc ctcctcagat ttcccttcaa ttttaatacg cccctgaaga
     1681 tacgccatct caattctttt tttcttcagc aaaattgctg cccacagacc cgcaggtacc
     1741 gttatcttca tatcccattt ttgcggcagt tctccctcct ttgccgttaa cacgctgtta
     1801 ttaatcatta tgctgtagca aaatccttta gtctcaccat caggttcaat attcagggcg
     1861 atagttaccg tccattgttt cactgcatca ggattatatc gctttaaaaa ctcatcggtc
     1921 aattcctttg cctgtggagc atagtgtgat gtgttgccag agagtccaat gcgctcaaga
     1981 aaacttttct ttggttttgc tgctgcaatg tctttacgat gcctgagata ataatacaat
     2041 gatggaaatg atgcatcaag ttcttcggta tatacttccc tgtgtacttt agcagacttt
     2101 tgtattgcta atgaaaaacg caggatatca cgcgcatctg cacctgaaag aactggttgt
     2161 gctttgccca gtattgattc aataaaatta tgagttgcac caataaatcc caatgcccag
     2221 tcggattttt cgttatagtg tttccaacct ttagtggtga atacactcac agcaggccct
     2281 ttgacaatat tgccagtgca tcggtgtata atgaccatac cctgagttcc gcttacctct
     2341 atccattcat cattggcata atatttggaa ggaattttaa gcgccggcat atgcacaaac
     2401 tcaattgagc catatacacc atttttatgc ttccatatca tcacagaagg agcatcaata
     2461 atgccatcga ttgagtcaat ccatccactg gcacgctcaa cgttcccaca taaataccag
     2521 gcagttgtcc acaggtgatg cccatggtca aatgtatcaa atccgcgaac accgccactt
     2581 tcagtatttt ctttcagtct ccattgccag gcttcaggtg gcacatacca gccaccactt
     2641 ccacccccaa tgaatttaat gcggatggct atggggttac ctatctctcc gttttctata
     2701 attttttttg caagccgtat gggtgggtaa aatgcatagt tatcggttac tttaaaaatt
     2761 ttgcctgact ctcttgttgc tgcaagtata cggtcagcac ttgcaagatc aacagtcatt
     2821 ggcttttgca gggcaatgtg ctttcctgct tttgctgccg caataaccac aggctcatga
     2881 agtgagtgcg gcgttaagat ttcaacagca tcaatgtgag gatcttttag caggtcatta
     2941 taatcagtgt atgcagcagt tgcatgccat tgtttagctc tttgctcaac cagcttctga
     3001 ttgatatcac atacagcata cagttttgca tgtttattat ttttatatcc aaggtaatga
     3061 aggtcagcaa tacgccccaa tccaatgaat gcaacattaa tgtgtttcat gacaccctcc
     3121 agtcattgca cattataaaa tgctattgca tatatgtcaa ttcatttttg ctgtcatgtc
     3181 caatatcgtt tcgtgctaca atagctacaa tcctcaatca ttccaggttt gatactgaat
     3241 ccacttgcat actgacattc tgattcatgt ccacgataat tctattcccg gtaactatcg
     3301 cccaccaatt atctaatttt ttcttaaaaa aatattgata ttgacacatt catagagtat
     3361 cttattgtgg tagtatataa ttgctgtgtt aactatattt ttggtagcat gtaatgctgt
     3421 aatcacctgc ccacagtaac caacccacag gattattgca ttaacacaca aaaattgcag
     3481 caattaccaa ttaaaatatg taaggagttt ttatggtaac agaaggtaaa tatgtcgcca
     3541 ttcattacac cggaaagttt gatgacggtc aggtgtttga ttcaagttta gacggtgcac
     3601 cttttgaatt tcaggccgga gctggcatgg ttattccagg tctggacaga gcagtgttgg
     3661 gcatgaagct tgacgaagaa aaagatatcg ttattgcccc cgaagatggt tacggggatt
     3721 atgacgaaaa tctgttatat gcatttccac ttgaagaagt acagcagcag tttaagcccg
     3781 aaattgggat gactatagg
//
LOCUS       DAORYL010000156         3745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_16435_length_3745_cov_10.555014, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000156 DAORYL010000000
VERSION     DAORYL010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16435_length_3745_cov_10.555014"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="PLV81_15075"
     CDS             <1..1014
                     /locus_tag="PLV81_15075"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HOM11462.1"
                     /translation="EDSIWWAIVTSTTVGYGDKYPISRLGRIIGILTMFFGMALVGVI
                     TGNIASTLVEKQLKEDRGLKPVKIKNHYVICGWKKDMSMVLSSIAMRKEDFAGYDVVL
                     VCMAESEQIENLKSNIIFSNIHFVYGDHIDEHVLNRANIRYAKKVLVLADELGKSGQD
                     ADSRTVMSVMSIKAISKSVYVCAEIIDQKYERYLRLSGCDEVILTNNYSKNIIADVFA
                     KSGLAHVIGELIDVSTPVSLTTDEIPVHFINKPYGELVEYFARKNKILIRLLENTGNF
                     YDRKRLALQEAQKTPDISKLVDNLRIVKTMIPNYPVLNPDKNYKIKNFSKAVLIEGTS
                     EII"
     gene            1024..1539
                     /locus_tag="PLV81_15080"
     CDS             1024..1539
                     /locus_tag="PLV81_15080"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOM11463.1"
                     /translation="MTIQIDEALIKKLQKLSLFKGLDLNVIRDLAELCSYKKIKKGRT
                     VIKEGDLGDELYIIVNGEIEILKRTLQNEPYTVVAMNADDTPLYVGELAMIDKDKRSA
                     TVVAKRDCDCLVLKRKDFVAYGDANPRVGLILTRAIALQLAQRLRRTNDDVITLFSAL
                     VEEISGEAEGL"
     gene            1564..2028
                     /locus_tag="PLV81_15085"
     CDS             1564..2028
                     /locus_tag="PLV81_15085"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM11464.1"
                     /translation="MKNIIMVILLLVSLPLAAQEKRTMQQEADDKKFQQGLFFVSINE
                     HQKALNEFYEYLDIYVDGLHRAKAYRFIAAIYFDNSQYTKAVSIYKRLFEEFPLTDDG
                     MYSLFQIGICYTKMGFEDDAVKAFTQLIKEYPDSNYAKDASTMLELLKIANN"
     gene            2034..3179
                     /locus_tag="PLV81_15090"
     CDS             2034..3179
                     /locus_tag="PLV81_15090"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOM11465.1"
                     /translation="MSKNNYLKLARLAFMMNSQSSSSIPVTFTLLKVFDSVLSPQEVD
                     FLLSMGKDAATSDAIFKKVNMPYDKACEIFSSLIAKGMIWSKVYEGQELFSVSPIMLG
                     WFEVYLCNGSTTSEKREFAHYLTRLFNSWKKYNVTPLRQLRNWINKNNVPHNVIKPAS
                     PGKKIVINEAIVPETSQVLASTSINELIARYSDEGLIAVVHCFCRQWKTMEGIACRLK
                     MPLEACIVLGKFSEHAVKYGFGRFISKEEAAKIIDETKKKGGVHIVWHDKDQIDKEEI
                     AICNCCWDCCGVLGSYNRGINSLHFKTFYCAYIMQQEKCTGCGLCVTYCPVGAITLQN
                     NKPVIDKNVCIGCLQCYHKCKKDVIAIQQEEREVFLPLLKPSRIRVP"
     gene            3303..3385
                     /locus_tag="PLV81_15095"
     tRNA            3303..3385
                     /locus_tag="PLV81_15095"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3337..3339,aa:Leu,seq:gag)
BASE COUNT         1223 a          575 c          798 g         1149 t
ORIGIN      
        1 gaagattcaa tatggtgggc aatagtaacg tcaacaacag ttgggtatgg tgataagtat
       61 ccaatttcgc gattgggaag aattattgga atactgacaa tgttttttgg tatggcgctg
      121 gttggggtaa ttactggtaa tattgcttcc acgcttgtag aaaaacaatt aaaagaagac
      181 agagggctaa aacctgtgaa aatcaaaaat cattatgtaa tatgcggatg gaaaaaagat
      241 atgtcaatgg tgctttccag tatagctatg agaaaagagg attttgcagg gtatgacgta
      301 gtgctggtat gtatggctga atctgagcag attgaaaacc tgaaaagcaa tattattttt
      361 tctaatatac attttgtata tggtgaccac attgatgaac atgtattaaa cagggcaaat
      421 atcaggtatg ctaaaaaagt actggtgctt gccgatgagc tggggaagtc ggggcaggat
      481 gcagattccc gtacggttat gtcggtaatg tcaatcaagg caatttcaaa aagtgtttat
      541 gtatgcgctg aaattattga tcagaaatat gagcggtatc tgcgtttgtc gggatgtgat
      601 gaggttatac tgacaaataa ttacagcaaa aatattatag ctgacgtatt tgctaaaagt
      661 ggccttgcac atgttattgg tgaactaata gatgtaagta cccctgtttc attgacaacc
      721 gacgagatcc ctgttcattt tatcaacaaa ccatacggtg aactggttga atactttgcc
      781 agaaagaaca aaatactgat taggcttctt gaaaacacag gcaattttta tgatcgtaaa
      841 cggttagcat tgcaggaagc acagaaaaca cctgatatat caaagcttgt agataatttg
      901 cgaattgtaa aaaccatgat tcccaattac cctgttttaa accctgataa gaattacaaa
      961 ataaaaaatt tttcaaaggc tgtgttaata gaaggtacaa gtgaaattat ttgaggcaaa
     1021 atcatgacta tacaaattga tgaagcatta attaaaaaat tacagaaact atcgcttttt
     1081 aaaggtctgg atttaaatgt catcagagat ttagcagaat tatgcagtta taaaaagatt
     1141 aaaaaaggta ggacagtaat taaagaaggt gatttaggtg atgagttgta tatcatagtg
     1201 aatggcgaga tagaaatcct gaaaaggact ttgcagaatg agccatatac ggtggttgca
     1261 atgaacgctg atgatacgcc attgtatgtg ggtgagctag ccatgattga taaggataaa
     1321 cgttcagcca ctgtagttgc aaaacgtgat tgtgattgcc tggtgctcaa acgcaaagat
     1381 tttgttgcct atggtgatgc caatccacga gtgggattaa ttttaacaag agctatcgcc
     1441 ctgcaactgg cacaaagact aagaagaacc aatgacgatg tcataacgtt attcagtgct
     1501 cttgttgaag agatatcagg tgaggctgaa ggactataat agattcttta gagggagtta
     1561 gcaatgaaaa acataataat ggttatactg ctacttgtta gtttgccatt agccgctcag
     1621 gaaaagcgaa caatgcaaca ggaagctgat gataaaaaat ttcagcaagg tctgtttttt
     1681 gtttcaatta atgaacatca gaaagctctg aatgaatttt atgaatattt agatatttat
     1741 gttgacggat tgcacagggc aaaagcatat cgatttatag cagctattta ttttgataat
     1801 tcacagtata ccaaagctgt tagtatttat aaacgtcttt ttgaagaatt tcctttgacg
     1861 gatgatggca tgtatagcct tttccagatt gggatatgtt acaccaaaat gggttttgag
     1921 gatgatgctg ttaaagcatt tactcagctt ataaaagaat accctgattc aaactatgct
     1981 aaagatgcct caacaatgct tgaactttta aagattgcta ataactaaca ataatgtcaa
     2041 aaaataatta ccttaaatta gcacgactgg catttatgat gaatagccag tcgtcctcgt
     2101 caatacctgt cacatttaca ctgcttaagg tgtttgattc agtgttatcc ccacaggagg
     2161 ttgatttttt gctttccatg gggaaagatg ctgctaccag tgatgcaata tttaaaaaag
     2221 ttaacatgcc ctatgacaaa gcatgtgaga ttttttcatc acttattgcc aagggtatga
     2281 tatggtcaaa ggtatatgaa gggcaggaat tgtttagtgt ctcgccaatt atgctggggt
     2341 ggtttgaggt gtatttgtgt aatggcagca ccacatcaga aaaaagggaa tttgcacact
     2401 atcttaccag gctttttaat tcatggaaaa aatacaatgt aactccactc aggcaactta
     2461 ggaactggat caataaaaac aatgttcctc ataacgtcat taaacctgcc agtccaggta
     2521 aaaagatagt tatcaatgag gcgatagttc ctgaaacaag ccaagtccta gcatcaacat
     2581 caataaatga actgattgcc cgttacagtg atgaaggatt gattgccgta gttcattgtt
     2641 tttgcaggca atggaaaacg atggagggga tagcgtgcag attgaaaatg ccactggaag
     2701 cctgtatagt gttgggtaag tttagcgagc atgcggtgaa atatggcttt gggcgattta
     2761 tttcaaaaga agaggcagca aagattatag atgagacaaa gaaaaaaggt ggtgttcata
     2821 ttgtatggca tgacaaagat cagattgata aagaagagat agcgatctgt aattgttgct
     2881 gggattgctg cggtgtgttg ggttcatata acaggggaat aaattcattg cattttaaaa
     2941 cattttattg tgcatatatt atgcaacagg agaaatgtac tggttgtggt ttatgtgtta
     3001 catattgccc tgtcggagca atcacattac agaataataa accagtaatt gataaaaatg
     3061 tatgtattgg ttgtttgcag tgctatcata aatgcaaaaa agatgttatt gcaatacaac
     3121 aggaagaaag agaagtattt ttacctcttt taaaaccttc ccggatacgt gtgccataat
     3181 aaataataca aatttacgtt tgactattac tttatactac atttttcttg acataaaata
     3241 atgggaaaat aaaatgattt ataaatttgt gctaaaatag tgttattaaa ttcactttat
     3301 aagcccaggt ggtggaactg gtagacacgc atgcttgagg ggcatgtggg aatctcccgt
     3361 atgggttcaa gtcccgtcct gggcaatcaa taagactgct agttaagcag tcttatttgt
     3421 tataaatata tattgctctg accccaattt ctgcatggac tctgcatccc ctgtgttctc
     3481 cgcgtgattt attttttttt cacacagata tcactgagtg cgcgtagagg attattatgc
     3541 tctgacccta ttgtgattat ctatcttttt cttgaattga cggctatctt ttttattact
     3601 tatacttttt ttgtgcactt tcttttgtcc agacggcaac aaaagaaagt accaaagaaa
     3661 aatgcctttg tcttctgtat gtatttaatt cttgccagcg tgctgcggac gccttctaag
     3721 gcttttgtcc tcgcacgcaa cccta
//
LOCUS       DAORYL010000157         3738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_16479_length_3738_cov_8.249525, whole genome shotgun sequence.
ACCESSION   DAORYL010000157 DAORYL010000000
VERSION     DAORYL010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16479_length_3738_cov_8.249525"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..528
                     /locus_tag="PLV81_15100"
     CDS             139..528
                     /locus_tag="PLV81_15100"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOM11466.1"
                     /translation="MDTNILVRFVVRDDLRQGESVKTSFAISQKKGKYLFISNVVVLE
                     LLYVLESVYDYTRVEIVNAINALLKNNIFKFENHNLLSEFINLSKTTNLELSDLLVGL
                     ISKHAGCRITITFDKLDSKFNLFKLLS"
     gene            546..701
                     /locus_tag="PLV81_15105"
     CDS             546..701
                     /locus_tag="PLV81_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11467.1"
                     /translation="MKKYVCYCFKYTEEDIINDITNNGKSTILEKIVAAKLLNQCNCA
                     VNHPEKR"
     gene            800..1834
                     /locus_tag="PLV81_15110"
     CDS             800..1834
                     /locus_tag="PLV81_15110"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOM11468.1"
                     /translation="MDYLLLAVFGALVLLIILVILLLLRNQPSDITSQINAALANQFL
                     ALQSSINNELHSARQEIAASKDTIAQHTLKTIETIKEIGQTIHTITQQQEEAQRLGQS
                     LKDLLQAPKLRGNYGEAILEEMLERVLPKGIWETQYTIDGREKVDAVVKIKDMIIPID
                     AKFPRDNYQKYLNASTEEEKRSFWNAYVGDIKKQIKSIKEKYIKPEKGTSEFALIFIP
                     SEAIYYETIAEKNYLGMPSDLYEFAQDNKVIPVSPNTFYAFLQLVVVGSRNVEIIKKV
                     KHIQNGLLELERLFELFFAKYEEIGKNIEKAAESYRMGDSHIKRYRLKLENTLKLEEF
                     SSDDLPVKKD"
     gene            1981..2328
                     /locus_tag="PLV81_15115"
     CDS             1981..2328
                     /locus_tag="PLV81_15115"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOM11469.1"
                     /translation="MKTLDQISLKENERQAIVELKNAILAQYPEAEIILFGSKARGDS
                     SDESDMDLLILIDGDVDDFMRENIFDIAYIIELKYDVVFGIIVEAKVLWYSDKTKYIP
                     LYNSIRKDGVITQ"
     gene            2325..2729
                     /locus_tag="PLV81_15120"
     CDS             2325..2729
                     /locus_tag="PLV81_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOM11470.1"
                     /translation="MTNELLELIEYRKNRALETLEEAKILFNNDRLFATVNRIYYSLF
                     YAVIALLLVKNLSSSKHSGVKGLFNREFIKSGIVPIKYGEFYNKMFDFRQRGDYGDFV
                     EFKKEIVKEWLIMAEEFVMVIIKTIDSMKQPK"
     gene            2744..3112
                     /locus_tag="PLV81_15125"
     CDS             2744..3112
                     /locus_tag="PLV81_15125"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOM11471.1"
                     /translation="MKTLDQIPLKENERQAIVELKNAILAQYPEAEIILFGSKARGDS
                     SDESDMDLLILLDCDPMDWEMGDVDKIIESNVRDIIYNIQLKYNIIFNVIIESKKYYN
                     TPIFKAMPLSWNINKDGIII"
     gene            3109..3528
                     /locus_tag="PLV81_15130"
     CDS             3109..3528
                     /locus_tag="PLV81_15130"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOM11472.1"
                     /translation="MKDETLLLIKYRIERSKESLNAAKVLLDSDNLYSAINRVYYGMF
                     YAVNALLLTKNLSSAKHSGVMSLFFKEFINSGLIDKELGRLYSDIFDLRMKYDYKIPA
                     KITKEDVHNWLIKAGNFIEIVTDLTFKIIDHDNNEAK"
     gene            3515..>3738
                     /locus_tag="PLV81_15135"
     CDS             3515..>3738
                     /locus_tag="PLV81_15135"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOM11473.1"
                     /translation="MKQNNLMINLYTSNNLEYLAEQLGRHLYHEKKDILSPSTVIVQS
                     LGMQRWISLQLAKQFGVFLNCRFPFPENFA"
BASE COUNT         1383 a          457 c          714 g         1184 t
ORIGIN      
        1 aataaaaatc attcctgttt gcaaatccat tgaagacatg tatggtttgc ttgcacgaaa
       61 aagacaaaag aaaatatcga tagatgatat gaacaaaaaa ttaaaaaaag ccattattaa
      121 taaaaataga tgataggatt ggatacaaat attttggtaa gattcgttgt aagagatgat
      181 cttaggcagg gagaaagtgt aaaaacctcc tttgcaattt cccagaaaaa aggtaaatat
      241 ttattcattt caaatgttgt ggttcttgaa ctattatatg tattggaatc cgtttatgat
      301 tataccagag tagaaatagt aaatgccatt aatgcattgt taaaaaataa tatttttaaa
      361 tttgaaaatc ataatttact gtcagagttt attaatttat ctaagacaac aaatcttgaa
      421 ctgtctgatt tactggttgg attaatatca aaacatgcgg gttgtcgtat aactataacg
      481 tttgataagt tagattcaaa atttaatcta tttaaactat tatcgtagat gccagatgta
      541 acattatgaa aaagtacgtc tgttattgtt ttaaatatac cgaagaggat attatcaatg
      601 atatcacgaa taatgggaaa tctaccatac ttgaaaagat tgttgctgca aaactactta
      661 atcagtgcaa ctgtgcagta aaccaccctg aaaaaaggtg atgtttatct gatgtccgcc
      721 gggttgtgga caattgtaaa ggtaatagtt aatagtaaat gtgatgagtg aatcgtaagc
      781 ttaaacattg tgaggcagta tggactatct tttattagct gtatttggtg cactggtatt
      841 attgattata ttggtaatac tactgttact gcgtaatcag ccatctgata ttacatcgca
      901 gattaatgct gctcttgcca atcagttttt ggcactgcag tcatctatta ataatgagtt
      961 gcattctgct cgtcaggaga tagctgcttc aaaagatact atcgcacagc atacgttaaa
     1021 gacaattgaa actataaaag aaattgggca gacaattcat acaattacac agcagcagga
     1081 agaagctcag cggttgggcc agtcgcttaa agatttgctg caggcaccaa aactgcgcgg
     1141 caattacggc gaggccattc tggaagagat gcttgaaagg gtgttgccta aggggatatg
     1201 ggaaacacag tacactattg atggcaggga aaaggttgat gctgttgtta agataaaaga
     1261 tatgattatt cccattgatg caaaattccc tcgcgataat tatcagaaat atctaaatgc
     1321 atctactgaa gaagaaaaac gctcattctg gaatgcatat gtgggcgaca tcaaaaagca
     1381 aattaaaagt ataaaagaga aatacattaa acctgagaaa ggaacatcag aatttgcgct
     1441 catatttata ccatctgagg ctatctatta tgaaactatc gccgaaaaaa attatttagg
     1501 aatgccttct gatttatatg agtttgctca ggacaacaag gttataccag taagccccaa
     1561 cacattctat gcatttttgc aattagtggt agtaggttca cgaaacgtag agatcattaa
     1621 aaaggtaaaa cacattcaaa atggcttatt agaactggag aggttgtttg aattgttttt
     1681 tgcaaaatat gaagagattg ggaaaaatat agaaaaggct gctgaaagtt ataggatggg
     1741 cgatagtcat ataaaaagat acaggttaaa attggaaaac acgctgaaat tagaagaatt
     1801 ttcatctgac gacctgcccg ttaagaaaga ctgatcgtta ctattgagaa taacactgaa
     1861 tttactgtaa aaaattcttg aatgtttatt gtgaagtatg gtatggtgtg tgggatatgc
     1921 actactcatt atacttttac attgattata tggtataaca atgttacaga aggttactgt
     1981 atgaagacat tagatcaaat atcattaaaa gaaaatgaaa gacaggcgat agtggagttg
     2041 aagaatgcta tactggcgca gtatcctgag gcagagataa tattatttgg atctaaagca
     2101 cgtggggata gcagtgatga atcagatatg gatttattaa ttttgattga tggtgatgta
     2161 gatgatttta tgagggaaaa tatctttgat attgcatata taattgagtt aaaatatgat
     2221 gtagtttttg gcattatagt agaagcaaag gtattatggt attcggataa aactaaatat
     2281 attccgttat ataatagcat tcgaaaagat ggtgttatta cacaatgaca aatgaattac
     2341 ttgaattaat tgaatacaga aaaaacagag cattagaaac actggaagaa gcaaaaatac
     2401 tttttaataa tgataggctt tttgcaacag ttaatagaat atattattca ttattttatg
     2461 cagttatagc attattatta gttaaaaatt tatcttcatc aaaacatagt ggtgttaaag
     2521 gattatttaa tagagaattt ataaaatcag gtattgttcc aattaaatat ggtgaatttt
     2581 ataataagat gtttgatttt agacaacgtg gtgattatgg ggattttgtt gaattcaaaa
     2641 aagaaattgt taaggagtgg ctaataatgg ctgaagagtt tgtcatggta attattaaaa
     2701 caattgattc tatgaaacag cccaaataaa aatttattac attatgaaga cattagatca
     2761 aataccatta aaagaaaatg aaagacaggc gatagtagag ttgaagaatg ctatactggc
     2821 gcagtatcct gaggcagaga taatattatt tggatctaaa gcacgcgggg atagcagtga
     2881 tgaatcagat atggatttat taatattgct tgattgtgat cctatggatt gggaaatggg
     2941 tgatgttgat aaaattattg aaagcaatgt aagagatata atatataata ttcaattgaa
     3001 atataatata atttttaatg taattattga atcaaaaaag tattataata ctccaatctt
     3061 taaagcaatg ccattaagct ggaatataaa caaagatggt attattatat gaaagatgaa
     3121 acattattat tgataaaata cagaattgaa cgttcaaagg aatcactgaa tgctgcaaaa
     3181 gtgttattag atagtgataa tttatattct gctataaata gagtttatta tgggatgttt
     3241 tatgctgtca atgcattatt attaactaag aatttatcgt ctgccaagca tagtggtgta
     3301 atgtctttat tttttaaaga attcataaac tcaggattaa tcgataaaga acttggtagg
     3361 ctttattcag atatttttga tttacgaatg aaatatgatt ataaaatacc agcaaaaata
     3421 acgaaagaag atgtgcataa ctggttgata aaagcaggta attttattga aatagttact
     3481 gatttaacat tcaaaataat agatcacgat aataatgaag caaaataatt taatgattaa
     3541 tctttatacc agcaacaacc ttgaatattt agctgagcaa ttgggaagac atctttacca
     3601 tgaaaagaaa gatattctgt cccccagtac tgttattgtt caaagcttag ggatgcaacg
     3661 gtggatttcg ctgcaacttg caaagcagtt tggtgtgttt ttaaactgca gattcccttt
     3721 cccggaaaac tttgccca
//
LOCUS       DAORYL010000158         3711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_16595_length_3711_cov_13.287746, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000158 DAORYL010000000
VERSION     DAORYL010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16595_length_3711_cov_13.287746"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLV81_15140"
     CDS             <1..227
                     /locus_tag="PLV81_15140"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOM11474.1"
                     /translation="FNHKDTLFLFTDGIFEEFNPVQFEDGYNDLKTFIQKYSDTSAEE
                     TINAIKNHLEHNPLSIQQKDDELIIGIDFV"
     gene            479..2815
                     /locus_tag="PLV81_15145"
     CDS             479..2815
                     /locus_tag="PLV81_15145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOM11475.1"
                     /translation="MQKTLITGIQDTSLEVVQNILSTVQGISHIEYKNKKYSIVYDSS
                     VVSPLQILETLEAAGLYVEKEILQASIGGMSCVMCSKAVTRSVMDLEGILDVSVNYAT
                     GKATLIVTPHIVTTEDIKTKVEEAGYQFLGSDAGEQKPYNIRLLQIITGLIAGSILMA
                     MMYVPEIFSHWVAALLSMPVFVFISFHIFVHAYHALKNRVLSMDVMYAMGTGVSFVAS
                     VCATFGILPHEFVVYETAIFLATFLNIGKYLEDRARSRTSGTIKKLLSLKPDTATVIR
                     DNNEIEIKTEDVVVGDSIIVKPQSRVPVDGVIIKGAGYIDESMVTGESVPVYKKEGDR
                     VIGGTLNKNNLLVIQAQVLGSDSVLSRIISLVQQAQESKPAMQRFADRVVGYFIPVIL
                     IIAFVASVLWYVFTGNASFAFQVFVAVIVIACPCALGLATPTAVTVGLGRGAELGILI
                     KNAEALEKASNVTAVVFDKTGTLTTGKLTVIDVVALNEQHDELISLCATLERYANHPV
                     GDAVVQYARDNGVLFKDAEEVKVFEGKGVMGTIDGRNIVAGSLAFIKEGVDTGAIEHH
                     IIVFEKQGKTVLVVADSKPLGIITLADKPKQEARVTLQRLKTKGKKTIMITGDNRQAA
                     DAIAKEVGVDDVYSDVTPEAKMNIVMKLQNNGDVVAFVGDGINDAPVLAQADIGIAMG
                     AGTDVAIEAGDVVLLRNRLADVVTYFDLAVKVMKRIKQNIFWAFAYNMLLVPVAAGIL
                     YPFTGFLIKPEFAGAAMALSSVTVVTLSLALKRFKPGK"
     gene            complement(2799..3158)
                     /locus_tag="PLV81_15150"
     CDS             complement(2799..3158)
                     /locus_tag="PLV81_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11476.1"
                     /translation="MEDNIDFFINDIKVSFVFFPFENIEKTEKINNITIASDIDLFLN
                     KIYVAGRRIDPKDPFDAAFLYSKNQWDKVYIEECFGKKFHGQSYKIYLGAICSFDDYG
                     DLPDWVKDVLLSLTSPA"
     gene            complement(3346..>3711)
                     /locus_tag="PLV81_15155"
     CDS             complement(3346..>3711)
                     /locus_tag="PLV81_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11477.1"
                     /translation="SYKNIIANCKHMTTIVVNYFINNLTITMSEHNQSNRIKWKYMQK
                     APIPSTIKQLLWDEQTVAPLEKIIMRVLQYGNYDEIKYIYGMYPEEVTEIVNRYTGLR
                     RGVKFWVVYWNKLYGHTNN"
BASE COUNT         1119 a          587 c          793 g         1212 t
ORIGIN      
        1 attttaacca taaggataca ttgtttttat ttactgatgg tatttttgaa gaatttaatc
       61 cagtgcagtt tgaggatggg tacaatgacc taaaaacttt tatacagaag tatagtgata
      121 caagtgcaga agaaactatt aatgctatta aaaaccatct ggagcataac cccttaagca
      181 tccagcaaaa ggatgatgag cttattatag gtattgattt tgtataaatg aaaagcttat
      241 tttaatattt gtactattgc atgttgacaa tgatataaat caaaagatta tgcttttttt
      301 taaaaatttt atgctctatc attttttgcc agtgtttttc atcccgcaat ctcgtaatct
      361 ctttaacacg ctatgattat ttgttttatg caaataacat cttttgtaaa tctattgttg
      421 attttatagt gtaggtgtag tatattatac taattaagaa tttaagagga atcttaatat
      481 gcaaaaaaca ctcattactg gaatacagga tacttccctg gaggtggtgc agaatatatt
      541 atccactgtg cagggtataa gccatataga atataaaaac aaaaaataca gtatagtgta
      601 cgatagttct gttgtaagtc ctctacagat actggaaaca ttagaagctg ccggcttata
      661 tgtggaaaaa gaaatactgc aggcttccat tggagggatg agttgtgtta tgtgctcaaa
      721 agcggtcact cgtagtgtta tggatttaga aggtattctt gatgtttcag ttaactatgc
      781 aacggggaaa gccacactga ttgttactcc ccacatcgtt accactgaag atattaaaac
      841 aaaagttgaa gaagcagggt atcagttttt aggctcggat gccggagagc aaaaacctta
      901 taacattcga ttgcttcaga ttattaccgg gcttatagct ggaagtatac ttatggcaat
      961 gatgtatgtc ccggagattt tcagtcactg ggtggctgct ttgctctcta tgcctgtatt
     1021 tgtgtttata agttttcata tttttgtcca tgcctaccat gcacttaaaa acagggtttt
     1081 atccatggat gttatgtatg ccatgggcac gggtgtatcg tttgtagcaa gtgtatgtgc
     1141 tacgtttggt attttaccgc atgagtttgt tgtgtatgaa accgctattt ttttagcaac
     1201 atttcttaat attgggaagt atttagagga tagagccagg tcaagaacat cagggaccat
     1261 taaaaagtta ctatcgctta aacccgacac ggctactgtt atacgtgata acaatgaaat
     1321 tgaaattaaa actgaagatg tcgttgtggg agacagtatc attgtaaaac cacagtcgcg
     1381 tgtaccggtt gatggtgtga ttataaaagg tgctgggtac attgatgaat caatggtaac
     1441 tggtgaatcg gtgccggtgt ataaaaagga aggtgacagg gttattggtg gaacactgaa
     1501 taaaaacaat cttcttgtaa tacaggcaca ggtactggga agcgattctg tactctcacg
     1561 gattatttca ctggtacagc aggctcaaga gtcaaaacca gcaatgcagc ggtttgctga
     1621 cagagtagta gggtatttta ttcctgtcat actgattatt gcttttgtag cttcggtgtt
     1681 gtggtatgtg tttaccggca atgccagttt tgcgtttcag gtttttgttg cagttatcgt
     1741 tatagcctgt ccatgcgcgc tgggacttgc aactcccact gctgtgacgg ttggtcttgg
     1801 gaggggtgct gagttgggga ttcttattaa aaatgctgaa gcactggaga aggcaagcaa
     1861 tgttactgcc gttgtctttg ataaaacagg cactcttact acaggaaagc ttactgttat
     1921 tgatgttgtg gccctcaatg agcagcatga tgaacttatt tctttatgtg caacgcttga
     1981 acggtatgcc aatcaccctg ttggagatgc ggtggtgcag tatgccagag acaacggtgt
     2041 gcttttcaag gatgcagagg aagtgaaggt ttttgagggc aaaggtgtaa tgggtacgat
     2101 agatggaaga aatattgttg ctggcagttt ggcttttatt aaagaagggg ttgataccgg
     2161 agctatcgaa caccatatta ttgtgtttga aaaacagggt aaaaccgtac tggttgtagc
     2221 agattcaaaa ccactgggta ttattactct tgctgacaag ccaaagcagg aggccagggt
     2281 aacattacaa aggcttaaaa ctaaagggaa aaagacgata atgattaccg gtgacaacag
     2341 gcaggcagcc gatgcaatag ctaaagaagt tggtgttgat gacgtatatt ctgatgttac
     2401 tcctgaagca aagatgaata ttgtaatgaa gcttcaaaac aatggtgatg ttgtggcttt
     2461 tgtgggagat ggcattaatg atgcaccggt gttagcgcag gctgatattg gcattgccat
     2521 gggtgcaggt acagatgttg ccattgaagc aggagacgtg gtgttattgc gaaacaggtt
     2581 agctgatgtg gttacgtatt ttgatttggc agtaaaagtt atgaaaagga ttaagcagaa
     2641 tatattctgg gcatttgcat acaatatgct actggtacct gttgcagcag gaatactgta
     2701 tccatttaca ggatttttaa tcaaaccaga atttgcaggc gctgcaatgg cattgagttc
     2761 agttactgtg gttacgttgt cacttgcact gaaaagattt aagccgggga agtaagggaa
     2821 agtaagacgt ctttaaccca atcaggaaga tcaccgtaat catcaaaact acatattgca
     2881 cctagataaa ttttgtagct ttggccatga aatttttttc caaaacattc ttctatataa
     2941 accttgtccc attgattctt tgaatacagg aaagcagcat caaagggatc ctttggatca
     3001 attcttctcc ctgctacata gattttattt aaaaaaagat ctatatcact tgcaatggta
     3061 atattattaa ttttttcagt tttttctata ttttcaaaag gaaaaaaaac aaatgatact
     3121 ttgatatcgt ttatgaaaaa gtctatattg tcttccattc gttcccacct attaatatgg
     3181 cctataaata aatttatcat tttctttgat agtcttgtat atgaaaaagc ccgaaaactg
     3241 aaaaaatcca gatcataact aatacggtga ttgtacttaa tcatgatggc tgtaccgccc
     3301 gcaagataga actttgggaa ataactctgt attttttgag ctaagttaat tattggtatg
     3361 tccatacaat ttgttccaat ataccaccca gaattttacc cctcgccgca aaccagtata
     3421 acgattaact atttcagtta cttcttcagg atacattcca taaatatatt ttatttcatc
     3481 ataattacca tattgtagta ccctcattat aattttttca agtggtgcaa cagtttgttc
     3541 atcccacaga agctgtttta tagtacttgg gataggggct ttttgcatgt atttccattt
     3601 tatgcgattt gattggttat gttcactcat tgtaatagtt agattattga taaaataatt
     3661 gactactatt gtagtcatgt gcttacaatt tgcaattata tttttgtaag a
//
LOCUS       DAORYL010000159         3592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_17320_length_3592_cov_7.474696, whole genome shotgun sequence.
ACCESSION   DAORYL010000159 DAORYL010000000
VERSION     DAORYL010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17320_length_3592_cov_7.474696"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            505..2472
                     /locus_tag="PLV81_15160"
     CDS             505..2472
                     /locus_tag="PLV81_15160"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOM11478.1"
                     /translation="MSGYMGQYVIINLSTGKSTVKKLDDSFYKKFLAGYGMGVAIVSQ
                     LQKPKINPLSPESVLGFCSGLLTGTGAYFAGRFMVVGKSPLTGGWGDSNCGGFFSVEI
                     KRAGYDAIFITGKAKKPVYLVINDGKVQIKDAKKLWGKDTIETEELIKKELKDEKFQV
                     ACIGPAAEKLSLISGIVTDGGRIAARSGLGAVMGSKNLKAIAIKGTQKIPVAKPDVLK
                     EINKKFLASFKSTSPIDKATVNMMNFLSQIIARTRVSVPTQPSTVREIFKRYGTSGTT
                     VYSAFTGDTPIKNWAGVGYLDFPGVQKISDESVIAHQKKRYACQSCPLGCGGIVDIKA
                     GRYKGTEGHKPEYETLGSFGAMLLLDDLDTIMEINELCNRAGIDTISTGTAVAFAIEC
                     YEHGILTKKDTGGLELGWGKAKEILALTEMIINRKGIGNILADGVMRAAKKIGKGSEA
                     YAIHAGGQELPMHDSRLDDGFAVTYSSEATPGRHTIASNLYNHLYRTKKQFPKVKKML
                     KNANSSTAKRLTLTAASMYFANLFNGCGMCQFGFLSGSIPLVDYINAVTGWDLPADDY
                     LTTGERILNMRKAFNVREGLYVKNTIIPERAQGKPPLQEGPLKGITIPIDEMLNEYFA
                     ITGWDSRTGGPTDTKLRELSIDDLFSTKKGK"
     gene            2757..3371
                     /locus_tag="PLV81_15165"
     CDS             2757..3371
                     /locus_tag="PLV81_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11479.1"
                     /translation="MRRIVCVVLSLVISIFIIGCSGSESSTEKSKFEVMADVLNAIGN
                     AGMDESGYNQRSMGRSIEGEDELNDQFGPETYEIAPGFSVTISGSINFTDTEINITFS
                     ADSTLTNYTSDGYILNGNLDETATAIITSNDQEITGMTEELIIDGTIEFSGKETGSFS
                     FDNLTISFVIDPQTGNVTSTNVSGTVTFNEQDVTSELIAELQAV"
BASE COUNT         1202 a          538 c          744 g         1108 t
ORIGIN      
        1 ctgttactcc agccccctat taatatccca agatttgtga tgggtaaaga aagatttggg
       61 gattccttaa aaagggatcc ccttttttaa ttttccacgg atcagaaaat ctggtttaca
      121 ataaaaaatc atatgctgtg gaacagaaaa acgatgtggg tatggagagg caattttttg
      181 accagaataa atatttacta aaaaactaat tagttattca tcatgttttg attaatgtct
      241 cttaaaattc tttaaataca tgttatgact gaagataaaa gcatttctgc ttcttgtggt
      301 ggtgaaggtt tttatgataa tgagcatagt acccaagatt acattaatcc tgattaattt
      361 ttaaaatatt tttttcttgt aattatctac aaaatacttt cacatagtat aacctattag
      421 tttgcgttaa agggtaatgg tatccactaa atgctatgta tttatggcat agtgatatat
      481 ttttctaaaa tcaggaggtt tattatgagt ggttatatgg gccaatacgt tattattaat
      541 ttatctacgg gaaaatctac tgttaaaaag cttgatgaca gtttctataa aaaattcctg
      601 gcagggtatg gcatgggtgt tgcaattgtg agccaattgc agaagccaaa aattaatcca
      661 ctgtcaccgg aaagcgtact tggattttgt tcaggcctat taacaggtac cggggcatat
      721 tttgcagggc ggtttatggt ggttggaaaa tcgccgttaa caggaggctg gggcgatagt
      781 aactgtggag ggttcttttc cgtagaaatc aagcgtgcag gatatgatgc aatattcatt
      841 accggcaagg caaaaaagcc agtgtattta gtcatcaatg atggtaaagt acagataaaa
      901 gatgcaaaga agttgtgggg aaaggatacc attgaaacag aagaactgat taaaaaagaa
      961 cttaaagatg aaaagttcca ggttgcctgc ataggacctg ctgcagagaa actatcgctt
     1021 atatcaggta ttgtaaccga tggaggaagg attgctgcac gaagtgggtt aggtgcagtt
     1081 atgggaagca agaacttaaa agccattgct ataaaaggaa cacagaaaat accagttgca
     1141 aaacctgatg tattaaaaga aataaataaa aaatttttag caagttttaa atcaaccagt
     1201 cctattgata aagccacagt taatatgatg aatttcttaa gccagattat tgcacggacc
     1261 agagtttcgg ttccaactca gccttcaaca gtacgtgaaa tatttaaacg ttacggtaca
     1321 agtggaacaa cagtgtattc tgcatttacc ggtgatacgc caatcaaaaa ctgggctggc
     1381 gttgggtatt tagattttcc aggtgttcaa aaaatttctg atgaaagcgt catagcacat
     1441 caaaagaagc ggtatgcatg ccagtcatgt ccactgggat gtggtggcat tgttgatata
     1501 aaagctggaa ggtataaagg cacagaagga cataaaccag aatatgaaac tctgggatct
     1561 tttggtgcta tgcttttact tgatgattta gatacaataa tggaaataaa tgaattgtgt
     1621 aacagggctg gtatagatac catatctacg ggtactgctg tagcatttgc tattgaatgc
     1681 tatgagcatg gtatactaac aaagaaagac actggtggat tagagttagg ttggggtaaa
     1741 gctaaagaaa tacttgcatt aactgagatg atcattaaca ggaaaggaat tggcaacata
     1801 ctggctgatg gagttatgcg tgctgcaaaa aagataggca aaggatctga agcctatgcc
     1861 attcatgctg gcggtcagga acttcctatg catgatagcc ggcttgatga tggttttgcg
     1921 gttacatatt catccgaagc aacaccagga cgtcatacca ttgcatcaaa tttatacaat
     1981 catctataca ggacaaaaaa gcaattcccc aaagttaaga aaatgctgaa gaatgcaaac
     2041 agtagcactg caaaacgctt aaccctgaca gcggcgtcaa tgtattttgc aaatctgttt
     2101 aatggatgcg gtatgtgtca gtttggcttc ctaagtggtt caataccact ggttgattat
     2161 attaatgctg tgacgggatg ggatttgcct gcggatgatt atttaacaac cggtgagcgt
     2221 atccttaata tgcgaaaagc tttcaatgtg cgtgagggtt tatatgtaaa gaatacaatc
     2281 atccctgaac gtgcacaggg caaaccacca ctacaggaag gtccgttaaa agggattacc
     2341 attcccattg atgaaatgct gaatgaatat tttgctataa caggatggga ttccagaacc
     2401 ggtgggccaa ctgatactaa attaagagaa ttgagtattg atgatttatt cagtactaaa
     2461 aaaggcaaat aattataatt tttaatacta ttataaaaaa tgcttgacta aaattataat
     2521 ttgttgttta ttaactaact ggaagggatt tcccagctca tttgttaata tagattataa
     2581 cacatagatg aaagaaagtt tatagttaac ttacgttaaa tttattaatt caattttatt
     2641 gtgatgtcac attaaatagc gaactattgt tatctgcttt ttagttatgt ttaattaaaa
     2701 tttgcatagc atctttttat taaatataat tacattaaaa aggagggttt tcccaaatgc
     2761 gaaggattgt ttgtgtagta ttaagtttag ttatttcgat attcattatc gggtgtagtg
     2821 gaagcgaaag ctcaacagaa aaatcaaaat ttgaagtaat ggctgatgta ctcaatgcaa
     2881 taggtaatgc tggaatggat gaaagtggtt ataatcagag gtcaatgggt cggtcaattg
     2941 aaggtgagga tgaattaaat gatcaatttg gacctgaaac ctatgagatt gccccgggtt
     3001 tcagtgttac aattagtggg tctataaatt ttacggacac agaaataaac ataacattta
     3061 gtgctgattc aacgttaaca aattatacca gtgatggata tattttaaat ggtaatttag
     3121 atgaaacagc tacggctatc attactagta atgatcagga aataacagga atgacagaag
     3181 agttaattat tgatgggaca attgaattta gtgggaaaga aacagggtca ttcagttttg
     3241 ataatcttac aattagtttt gtaatagatc cacaaacagg gaatgtaacg tcaaccaatg
     3301 tttcaggtac ggttactttt aatgagcagg atgttaccag tgaattgata gctgaattac
     3361 aggctgtata attataatac tataaaaaaa gttgtaatta aaaagggcag gaaatcctgt
     3421 cctttttaat tgattgctat gctctgaccc ctgtttactc atactgcatt tagatggtca
     3481 tcctcactgt aaccgtcttc cctgagttat tgctctgaac cttctttcac ttttttcttt
     3541 tttttacacc caatccacta catacgaata ttttttccga taatactcct ca
//
LOCUS       DAORYL010000160         3512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_17819_length_3512_cov_11.530518, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000160 DAORYL010000000
VERSION     DAORYL010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17819_length_3512_cov_11.530518"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            311..1531
                     /locus_tag="PLV81_15170"
     CDS             311..1531
                     /locus_tag="PLV81_15170"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOM11480.1"
                     /translation="MKTASIPELIEDIRNGKMIILVDNEDRENEGDLIVAAQYCTPDK
                     INFMATHGKGLICIAMTQERLQELGLPLMVPENSDKFCTAFTVSVDAKEGTTTGISAY
                     DRAKTVHVLIDPRTKPQDLNKPGHIFPLAAKKGGVLVRAGHTEGSLDLTRLAGLYPAA
                     VMCEILNDDGTMARRPDLDKFAEKHNLKIGTIEDLIKFRQHKERLVRRVSEAHLPTEY
                     GDFTIIAYETEVDNATHVALVKGEVDGRQNVLVRVHSECLTGDVFGSHRCDCGSQLHQ
                     ALRMIEREGLGVLLYMRQEGRGIGLSNKLKAYHLQEQGYDTVEANIKLGFPADLRDYG
                     IGAQILVDLGLSTIRLMTNNPKKVIGLEGYGLTIVERVPIEIQPIKENYHYLKTKKDK
                     MGHLILQSSEEKKS"
     gene            1528..2475
                     /locus_tag="PLV81_15175"
     CDS             1528..2475
                     /locus_tag="PLV81_15175"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM11481.1"
                     /translation="MKNGQPPTIVVVDDDEVIRYSLQKKLSRLGYNIISLSKAEDALF
                     LLKNDERDIDLIITDIKLRKMDGIELLRHINTSENPVPTLIITGQGNIEDAIKALRYG
                     AIDFIRKPFDINEVASIVRNILRRKQQHSLENRLGEYIIHDQRTLVIPVDETIADIVA
                     YELTKHLPYAGFCNRSTAENISMALLEALSNAMYHGNLEIPSEVREEQGMKGFNDEIQ
                     KRLQDPQFNNRKVTIHYHYTGDYVEYIIEDEGKGFDYTNVADPRDPENFLKNSGRGLF
                     IIQVHMDEVAWEKGGRQIRMRKYRTIQRGGNNVLYHTLG"
     gene            2447..3484
                     /gene="mtnA"
                     /locus_tag="PLV81_15180"
     CDS             2447..3484
                     /gene="mtnA"
                     /locus_tag="PLV81_15180"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOM11482.1"
                     /translation="MMYYTIRWDESAVSMLDQRMLPVQEVYRHYTNYHDVADAIRSMV
                     VRGAPAIGIAAAMGIALAAVKSKTNNINDFKSEMLQVCQIFASTRPTAVNLFWAINRM
                     RNIIEKADNIDMCKNDLVREAQKIHQEDIEINKKIGEFGQKYIEDGITVMTYCNAGAL
                     ATGGYGTALGVIRSAVEHGKKIKVYACETRPYLQGARITAWELFKDGIDTTLICDNMA
                     GHFMKKGEIHCVIVGADRIAANGDTANKIGTYTMSILAKAHGIPFYVAAPISTLDPDT
                     PDGNSIVIEERNIDEVAYIQGVQIAPEGIFIKNPAFDVTPAANITAIITEKGVIENPV
                     ATGIAPLVNVR"
BASE COUNT         1188 a          555 c          779 g          990 t
ORIGIN      
        1 taaaccccaa tacattattt gaaattcaac caatgaatag tatatttgta tatatagttt
       61 tgagtcaact aaatatgaaa acttttgtta attatttcat gaattatttt taatatttat
      121 atgtacaaga attgtgttct tctgctgctt aaagtggcag agctttatat tatttcacat
      181 gtagcttata tggtaatatt atggaggaat acgtagtgta ttattttaca ataaatggca
      241 aaatgtcaga atataaaact cttgactaca gtaggaatat ttttttagtg tacatcacag
      301 gagaaaatat atgaaaactg ccagtatccc cgaattaatt gaagatatac gcaatgggaa
      361 gatgataatc cttgttgata atgaagacag ggaaaatgaa ggtgacctta ttgttgcagc
      421 ccagtattgt acacctgata aaataaattt catggcaact catggcaaag ggctgatatg
      481 tattgcaatg acccaggaga gattgcagga attaggtttg ccgctgatgg tacctgaaaa
      541 ttctgataaa ttctgtactg catttactgt ttcggttgat gccaaagagg gaactacaac
      601 cggcatatcg gcctatgaca gggcaaaaac tgttcatgta ttgattgacc cacgtacaaa
      661 accacaggat ttaaataaac caggtcatat attccccctt gctgcaaaaa aaggaggtgt
      721 gctggtaagg gcggggcata ctgaggggtc gctagattta actcgtttgg cgggtcttta
      781 tcctgctgca gtgatgtgtg aaattttaaa tgatgatggt accatggcac gccgtccgga
      841 tttagataaa tttgctgaaa agcataatct taaaattggt acaatagaag atttaattaa
      901 attcaggcaa cataaggaac gtttagttcg tcgagtatcc gaagcacatc tgccaaccga
      961 atatggtgat tttactataa tagcatatga gactgaggtt gataatgcaa cgcatgttgc
     1021 tcttgtaaaa ggagaagtgg atggcaggca aaatgtactg gtgcgcgttc attcagaatg
     1081 tttaaccggt gatgtatttg gttcacatcg ttgtgattgt ggatctcaat tgcatcaggc
     1141 attgcgcatg atagaaagag aaggccttgg tgtattatta tatatgcggc aggaagggcg
     1201 cggaataggt ttaagtaata agctcaaagc atatcatcta caggaacagg ggtacgatac
     1261 ggttgaggct aatataaaat taggattccc tgccgattta cgggattacg ggattggtgc
     1321 tcaaatactt gttgatttag gattgtcaac tatccgttta atgacaaata accccaagaa
     1381 ggtgattggt cttgaagggt atggccttac tatcgtagaa agggttccaa tagaaataca
     1441 gccaataaaa gaaaactatc actatttaaa aacaaagaaa gataagatgg gtcatctgat
     1501 actacagtct tcagaggaaa agaagtcttg aaaaatggtc agccgcctac aatagttgtt
     1561 gttgatgatg atgaggttat tcgttatagc cttcagaaaa agctttccag gctaggctat
     1621 aatattattt cgttaagtaa agctgaggat gccctttttt tattgaaaaa cgatgagcgt
     1681 gatattgatt tgataatcac ggatattaaa cttaggaaaa tggatggcat cgaattattg
     1741 cgtcatatta acacaagtga aaatccggtg ccaacgctca ttatcactgg tcagggtaat
     1801 attgaagatg ctatcaaagc gttacgatat ggtgccatag attttatacg caaacccttt
     1861 gatatcaatg aagtcgcttc aattgtaaga aacattttac gcaggaaaca gcagcatagc
     1921 ctggaaaacc ggttaggaga atatattata catgatcaaa gaacactggt catacctgtt
     1981 gatgaaacta tagcagatat tgttgcatat gaattaacaa aacatctgcc atatgcagga
     2041 ttttgcaatc gttcaactgc tgaaaatatt tccatggctc ttttagaagc actttccaat
     2101 gccatgtatc atggaaatct ggaaatacca tctgaagtaa gagaagaaca gggaatgaaa
     2161 gggtttaatg atgaaataca gaagcggcta caggaccctc agtttaataa cagaaaagta
     2221 actattcatt atcattatac tggtgattat gttgaatata taattgaaga tgaaggcaag
     2281 ggatttgatt ataccaatgt tgcagaccca agagatccgg aaaatttttt aaaaaacagt
     2341 gggcgcggtc tttttataat tcaggtccac atggatgaag ttgcatggga aaaaggtgga
     2401 agacagatac gaatgagaaa atacaggaca atacagcgag gagggaataa tgtactatac
     2461 catacgctgg gatgaatcag cagtttcaat gttggatcaa cgcatgttac cagtacagga
     2521 ggtatacaga cattacacaa actatcatga tgtagcagat gcaatacggt caatggtagt
     2581 cagaggagct ccggctatcg gtatagccgc agccatgggt atagcactgg cggctgttaa
     2641 gagtaaaaca aacaatatta atgattttaa atctgaaatg ctgcaggtat gccagatttt
     2701 tgcatcaaca cggccaactg ctgttaatct tttctgggca atcaacagga tgaggaatat
     2761 aattgaaaaa gcagacaaca ttgatatgtg taaaaatgat ctggtgcgag aagcacaaaa
     2821 aattcaccag gaagatattg aaataaataa aaagattggg gaatttggtc aaaaatatat
     2881 tgaggatggc attacagtaa tgacctactg taatgcagga gcactggcta ctggtgggta
     2941 tggtacggca ctgggagtaa ttagaagtgc tgttgagcat ggaaagaaaa taaaagtata
     3001 tgcatgcgaa acaagaccgt acttgcaggg tgcacggatc actgcatggg aacttttcaa
     3061 agatggtata gacaccactc ttatctgtga taatatggct ggtcatttca tgaagaaagg
     3121 tgaaatccat tgtgtcattg ttggagcaga caggattgca gcaaatggtg acactgcaaa
     3181 caagattgga acatacacaa tgtctatact ggcaaaagca catgggatac ctttttatgt
     3241 ggcagcccct atatcaacac tggatcctga tacacctgat ggcaacagca ttgtcattga
     3301 agaacgaaat attgatgaag ttgcgtatat tcagggagta cagatagctc cagaaggaat
     3361 attcataaaa aatccagcat ttgatgttac tcctgctgca aacattacgg ccattattac
     3421 agaaaaaggt gttattgaaa atccggttgc aacaggtatt gcaccacttg taaatgtgcg
     3481 atagttactg ttattaagct ctgacccctt tt
//
LOCUS       DAORYL010000161         3452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_18225_length_3452_cov_11.266412, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000161 DAORYL010000000
VERSION     DAORYL010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18225_length_3452_cov_11.266412"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..734)
                     /locus_tag="PLV81_15185"
     CDS             complement(144..734)
                     /locus_tag="PLV81_15185"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOM11483.1"
                     /translation="MDKQEKIQKKKAIILEALKRLLQNDVYSRITVQSVADEAGFSKG
                     GLLHYFPTKEEMYVEFMEYLFSEINNDHKNVLKGNLHSKAKASISAMYGVERFFLDPG
                     TAKIFINLVLYAYEDEKIMIRLKEFIRRHLELYKTIIDEAREDLPKRRKTDLDAEFHA
                     RIAQIIVICSGLFESIDPLPMDSFNLMRYIISLFRG"
     gene            1029..>1979
                     /locus_tag="PLV81_15190"
     CDS             1029..>1979
                     /locus_tag="PLV81_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11484.1"
                     /translation="MAKRAIITKITISLCVVFSCFYYVYAQFLMEDYKKSEKKPKPGL
                     PPYIARNIKAEVINEVEGAVKITFSVDSDSTNDFLVGRTTQLPSSSEIALQAATVKVV
                     LAGAEPVAIDSNLPPGQYYYVVLARDKILEKDIALYPDVNYTSAPIVIKKKIPEVPQK
                     RLPEQVTLLYARVVNKTQVLLTWKGVQQPNIVYNVYRSTEPLNSPESIKNAKLIGQVQ
                     ADKESFIDRTITQSGKYFYAVTVQDIAGNEDVQLIPDQSYTANPVSVIIRRDVFVTNI
                     RADIDEGALKITWSKPDVDVKSFVIYRHTQPIIDAQTLAMA"
     assembly_gap    1980..2079
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2259..>3452
                     /locus_tag="PLV81_15195"
     CDS             2259..>3452
                     /locus_tag="PLV81_15195"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM11485.1"
                     /translation="MKTKTLTGALLILIGVCLLIYYVQVIQPQNEAKKLLTEATLIYE
                     RGDKESINQAVSILTKIIARYPDTKTAVEAYYYIGQCYEKLNLNRLAYLKYVYLVKNN
                     RKDLSDDLYKNILARIAHINWLQNYNEESAHQLLTLLNSSLDKGYRSKIYSELGHLYL
                     KTGEVAKAKRMFDIAVNEDSSNEEAILGRARALKHLGMDNEAYNHYDYFLRYFGAVSQ
                     YTSDVKNAYKIQAYKSGLAAFRNGRYNEAIGFFNRVLTYFGGDRLSENALYWTGESYF
                     AMRQFDRAIAYFNKALVNDYYHKDQDAQIKKGYAYFMQKKFDLAAREFQRYLREYPNG
                     KYVAVAKEWKNMSTTELQNIIEGKKSLEEGSVEEEVKPQPQQQKKKTKDEDLGLEEDE
                     EVSGSV"
BASE COUNT         1146 a          533 c          664 g         1009 t
ORIGIN      
        1 tacaggtatg gattgatttt caggcctgga atgacgtcgg taagggatat cggactaaaa
       61 ctgaatagaa aaaagaattt cgaatgaagc ctattaccat aaaagcaggg ggtctggaat
      121 gttttcttga ggttgttaaa ttatcatcct ctgaaaagag agataatgta tcgcataaga
      181 ttaaaactat ccatgggtaa agggtcaata gattcaaata aacctgaaca tataacaatg
      241 atctgggcta tacgggcatg gaattcagcg tccaaatcag ttttgcgacg ttttggcagg
      301 tcttctcgag cttcatctat tattgtttta tataattcta aatgccggcg tataaactct
      361 tttaacctga tcattatctt ttcatcttca tacgcataca gaacaagatt gataaatatc
      421 tttgcagttc ccggatccag gaaaaaacgt tcaacaccgt acatggcaga gatacttgct
      481 ttggctttag aatgcagatt accttttaac acatttttgt gatcattatt tatttcactg
      541 aaaagatatt ccataaattc aacgtacatc tcttctttgg tagggaagta atgaagcaaa
      601 ccgcccttag aaaaacctgc ttcatcagct acactttgaa ctgttatgcg tgagtaaaca
      661 tcattttgta ataaacgttt caatgcttca agtattattg ctttcttttt ttgtatcttt
      721 tcctgtttgt ccataacaat aaactatatc ataacattat gattaaaaaa ttgccacaat
      781 gatggcacaa ttacaacaat agaaaaacaa atattattta aacaaaagcc aatgcaaagg
      841 tcaagtattt ttcatagggt atcgattttt ttatatgatt tattcttaaa tttgtaataa
      901 tactttaagt gtagtattaa taattccgaa atagagtagt aggatgattt ctgccgtaat
      961 tatcatcttt tgcaagatac taaatctggt gtaataaaaa ttttggaatg ataaggataa
     1021 atttcttaat ggctaagagg gcaataatta caaaaattac tatctcacta tgtgtggtat
     1081 tttcttgttt ttattatgtt tatgcccaat ttttaatgga agattataaa aaaagtgaaa
     1141 agaaaccaaa accgggcctg ccaccctata ttgctcgaaa tatcaaagca gaagtaatta
     1201 atgaggttga aggtgcggta aaaattactt ttagcgtgga ttccgattca acaaatgatt
     1261 tccttgtagg gaggacaact cagttacctt cttcatcaga gattgccctg caggctgcaa
     1321 cggtgaaggt tgtattggcc ggtgccgaac cagtagctat tgattcaaat cttcctccag
     1381 ggcaatatta ttacgttgtg ttagcacggg ataaaatact ggaaaaggat atcgcattgt
     1441 atcccgatgt caattataca agtgctccga tagtaattaa aaaaaagatt cctgaagtgc
     1501 cccaaaaacg tttgcctgaa caagttacat tattatatgc acgggtggta aataagactc
     1561 aggttttgtt aacgtggaag ggtgtgcagc aacccaatat tgtgtataat gtttacagat
     1621 ctaccgagcc cttaaatagc cccgaaagta taaaaaatgc aaaacttatt gggcaggttc
     1681 aggcagataa ggaaagcttt attgatcgca ccattacaca gtcagggaaa tatttctatg
     1741 ctgtcacggt acaggatatc gccggcaatg aagatgtcca attaatacct gatcaaagtt
     1801 atactgcaaa cccagtttcg gtgattatac gccgcgatgt ctttgtcact aatatcaggg
     1861 ctgatataga tgaaggagcc ctgaagataa cctggagcaa acctgatgtt gatgtgaagt
     1921 catttgtgat ttacaggcat acccagccca ttattgatgc acagacgctg gctatggcan
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tgcaaaagcc aatcttttta
     2101 taggcaggag ttatattgaa ttgaagcagt ataaagttgc tcttaaatat attatgaaaa
     2161 gtgatgtggc aaggtactat cccaaagaag caaaattctg gagggacttt gcattatcaa
     2221 gagtacaata atttatgaaa agaagtcaag aggtatttat gaaaacaaaa acattaacag
     2281 gggcattgct tatacttatt ggtgtatgtt tacttatata ttatgtacag gtcatacaac
     2341 ctcaaaatga agcaaaaaaa ctgttaaccg aagccactct tatctatgaa cgaggtgata
     2401 aggagtctat aaaccaggca gtttccatac ttactaaaat tattgcccgc tatccagata
     2461 ctaaaactgc tgttgaggct tattattata ttggtcagtg ttatgaaaaa ttaaatttaa
     2521 acaggttagc atatcttaaa tatgtctatc ttgtaaagaa taacagaaaa gacctttcag
     2581 atgatctcta taaaaatatt ttagcacgta tagcccacat aaattggttg caaaattata
     2641 atgaagagag tgctcatcaa ctgttaacat tgcttaactc ttccctggat aaagggtata
     2701 gaagtaagat ttattctgaa ttaggtcacc tgtatcttaa aactggtgaa gtggctaaag
     2761 caaaaagaat gtttgatatt gctgttaatg aggatagcag caatgaagag gcaattttag
     2821 gtcgtgcccg tgcactgaag catctgggca tggataatga agcatacaat cattatgatt
     2881 actttttacg gtattttggt gctgtaagtc agtataccag tgatgttaaa aatgcataca
     2941 agattcaggc atacaaaagt ggcctcgcgg ctttcaggaa tggtcggtac aatgaagcaa
     3001 taggattctt taaccgtgta ttgacttatt ttggtggtga tcgattgtct gagaatgcat
     3061 tatactggac cggcgaatct tattttgcaa tgagacagtt tgacagagct attgcctatt
     3121 ttaataaagc actggtaaat gattattacc acaaagatca ggatgctcag ataaagaaag
     3181 gctatgcata ttttatgcag aaaaagtttg atttagctgc acgagagttc cagagatacc
     3241 tgcgcgaata tccaaatggt aaatatgtag ctgttgcaaa agagtggaaa aatatgagca
     3301 ccacagaatt gcagaatata atagaaggga aaaaatcctt ggaagaagga agtgttgaag
     3361 aagaagtaaa accacagcca cagcagcaaa agaagaaaac aaaagatgag gatttagggc
     3421 ttgaagaaga cgaagaggtt agtggttcag tc
//
LOCUS       DAORYL010000162         3415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_18481_length_3415_cov_14.580060, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000162 DAORYL010000000
VERSION     DAORYL010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18481_length_3415_cov_14.580060"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /gene="glpX"
                     /locus_tag="PLV81_15200"
     CDS             complement(<1..825)
                     /gene="glpX"
                     /locus_tag="PLV81_15200"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HOM11486.1"
                     /translation="MDRNLALEVVRVTEAAALWAARYYGRGNEELASKNAVEAMSKVF
                     STVSFKGHIISGINNPDSPLSNGKILGNADHPEVDIALTPIDGHATLAQGGYNTISAI
                     AIAEHGSMLNAPPIYMEKIAVGKEGRGVIDITQSPEVNIKRVARAKGKYIEDITVCIL
                     DRERHRELVERIRALGARIKLIKDGDISGAIATALDDTPIDMLMGVGGAREGILSAAA
                     LKCLDGDMQARFVYRDQKDKELVKAMGINDLEKIYTLSDLATGENIMFSATGISDGE"
     gene            complement(978..2120)
                     /locus_tag="PLV81_15205"
     CDS             complement(978..2120)
                     /locus_tag="PLV81_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11487.1"
                     /translation="MLVLTLRLPLSARGIPLDSVYISTSSAEYQKLFFTRLDYYQSIG
                     AQFIDSGIAYAGWLNGKEVVYIKESGNTNTVIINTVATRKKNTVYQFNGTVISARVSA
                     NGAYIAIKYFSIDTIPQPVLLLIHIPKKTIKVVKTASAGTDYSFSRDGNSLLYKNNNC
                     IVEVMGDSYREKEILCREDAPQWDENTVILDCSSHRLFISGSSGNYDVYMHANHVWKK
                     LFTTITPAEVFIANNTALYTAGFPGGYTVSQYSLQSGKTQRILSGSFNPSLHMIGNTA
                     IFLNNAFITLYDSSKNKLLPTTIESDEAIVSPDLFSIASTLYNRLFILPTVNILALDP
                     QSKRYLATVESHYTHISQKKSIYTSPYSYTYIQQKKKTIASLLTLF"
     gene            complement(2164..3399)
                     /locus_tag="PLV81_15210"
     CDS             complement(2164..3399)
                     /locus_tag="PLV81_15210"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOM11488.1"
                     /translation="MKQIYIYILVSCIVFSIGCASDLPIRDMSKARYGITEAEEVKAD
                     KYAPEELEKAKQYLYDTHSLLKEDKIKDAQKKSQESQAESQKAIEKSLPLYANDMLTE
                     AKETLQQAEMLNAKEFANVEYAQAVSSLNEATQLNTDKNYRQSIQKSKESIGFANEAK
                     AKSLAMIPQLKEQLVVLENEKESLRTQRGDEFAKDELSLTEQKINEATTKLEEQNIVA
                     AIAAMQSAKESLLLAKTAIEKGKASESLEAAKSLYTQVSERESSQEMAQTLTEAEKLI
                     ANSQDLFSKEKYIESYDASQQAITALNSMLIAMEKKEEAIAMQEEAKEETEVIAQEEP
                     KKEYKEYIVQYNPKARDCLWRIALKVYNNARLWPLIYIANKDQIKDPDLIFPGQRFII
                     PAVEKEKKESGTEEKAKSN"
BASE COUNT          868 a          677 c          714 g         1156 t
ORIGIN      
        1 ctcaccatcg gaaatgccgg ttgctgagaa catgatattc tcacctgttg caagatcgga
       61 cagcgtgtaa attttttcta aatcattgat gcccatggcc ttaacaagtt ctttgtcctt
      121 ctggtcgcgg tacacaaacc gtgcctgcat gtcaccatca agacatttaa gtgcggcagc
      181 cgaaagaatc ccttcacggg ctccaccaac acccataagc atgtcaatag gggtatcatc
      241 aagtgcggtg gcgatagctc ctgaaatatc accatctttt attaatttaa tccgtgcacc
      301 taaggcgcgt atgcgttcca ccagttcgcg atggcgctca cggtcaagta tacacacggt
      361 aatatcttct atgtactttc cctttgcacg ggctacgcgc ttgatgttaa cttcaggtga
      421 ttgtgtgata tcaataacac cgcgtccttc cttgcccaca gcaatctttt ccatgtagat
      481 tggaggtgca ttgagcatgg acccgtgttc tgcaatggca atagctgaaa tagtattata
      541 accgccctgt gccagcgttg catgtccatc aatgggggtc agagcaatat ctacttctgg
      601 atgatcggca ttacccagta ttttgccatt tgatagaggt gaatcgggat tattgatacc
      661 ggaaatgata tggcctttaa atgatacggt tgaaaacacc tttgacatgg cttcaacagc
      721 attttttgaa gcaagttctt cattccccct gccatagtag cgggcagccc acaatgccgc
      781 tgcttcggta acacggacaa cttccagtgc taaattgcga tccatagtat actccaccaa
      841 ccagtgtaac tacagttcat atcagtatga atacaatcaa aaattgtcaa attaatattt
      901 tataaaatat tcttatgtgg ggagtagaat gttgaacgta atgtattgtt gtaaagtgta
      961 ttaatggcta tccatcgtta aaataatgtg agtaatgatg ctatcgtttt ctttttttgt
     1021 tgtatgtaag tatagctgta tggggatgta tatatactct ttttttgtga tatgtgagtg
     1081 tagtgcgatt ctactgttgc aaggtatcgc tttgattgag ggtctaatgc aagaatattt
     1141 acagtgggta gtataaataa acggttgtac agcgtggaag caatggaaaa aagatcaggg
     1201 gaaacgattg cttcgtcgct ttcaatggtg gttggcaata gtttattctt tgagctatcg
     1261 taaagggtga taaatgcatt gttcagaaag atagcggtat tccctatcat atgcagtgat
     1321 ggattaaatg aaccagataa aattctctgt gtttttcccg attgcaatga atactggctt
     1381 accgtataac ctccgggaaa tcctgcagta tataaggctg tgttattggc aataaaaact
     1441 tcggccggag ttatggtagt gaaaagcttt ttccatacat gattggcgtg catatagaca
     1501 tcataattgc cggatgatcc actgataaaa agtctgtgac tgctgcagtc taaaattact
     1561 gtgttttcgt cccactgtgg tgcgtcttca cggcaaagaa tttctttttc acggtaacta
     1621 tcgcccataa cctcaacaat acagttgtta tttttataca acaatgaatt accatcccgg
     1681 gaaaaagagt aatccgttcc tgcagatgca gttttcacta ctttgatggt tttctttggt
     1741 atatgtatca agagcagtac tggctgtggt attgtgtcaa tgctgaaata tttgatggca
     1801 atgtatgcac cgttagctga aacgcgtgct gatatgacag tgccattgaa ttgatacact
     1861 gtgttttttt tgcgagtggc gacagtatta atgataacag tatttgtgtt gccagattct
     1921 tttatataga caacctcttt cccgttgagc catcctgcgt atgcaatccc ggaatcaata
     1981 aactgtgccc caatactctg atagtaatca aggcgtgtaa aaaataattt ctggtattcg
     2041 gctgaagatg tactgatgta taccgaatca agcggtatgc cacgtgcaga cagcggcagt
     2101 cgcaaagtaa gtaccagcaa gataataagc accactcgca ttggcaaatg gatgtgcagg
     2161 gtattaattg gattttgctt tttcttcagt gcctgattcc ttcttttcct tttccactgc
     2221 aggaatgata aaccgctgcc caggaaagat taaatcaggg tccttgatct ggtctttgtt
     2281 ggcaatataa atgagaggcc acaatcgtgc attgttataa actttaagag ctatcctcca
     2341 caggcaatca cgtgctttag gattatactg aacaatatat tctttgtatt ctttttttgg
     2401 ttcttcttga gcaatgactt ctgtttcttc tttggcctct tcctgcattg caatggcctc
     2461 ttccttcttt tccatagcaa taagcattga attgagtgcc gttattgcct gctgagatgc
     2521 gtcataggat tcaatatact tttcttttga aaagaggtcc tgactatttg ctataagttt
     2581 ttcagcttca gtaagtgttt gtgccatctc ctgggaagat tccctttcac tgacctgtgt
     2641 gtacaggctt tttgcggctt ccagggattc agatgctttt cctttttcaa tagctgtttt
     2701 tgccagtagc aaagattctt ttgctgattg cattgctgca attgctgcaa ctatgttctg
     2761 ctcttcaagt ttagtagtgg cttcattaat tttttgctct gttaatgaca gttcgtcttt
     2821 tgcaaattca tcaccacgct gggtgcgcag cgattctttt tcattttcca gtactacaag
     2881 ctgttcttta agttgcggaa tcattgccag tgattttgct tttgcctcat tggcaaagcc
     2941 aattgattct tttgattttt gtattgattg gcgatagttt ttgtcagtat taagctgtgt
     3001 tgcttcattg agggaactta ctgcctgtgc atactcaaca tttgcaaatt ctttggcgtt
     3061 aagcatttcg gcctgctgca aggtctcttt agcttctgtg agcatatcgt tagcatacag
     3121 tggcagggat ttttctattg ctttttgcga ttctgcctgt gattcttgtg attttttctg
     3181 agcatccttt attttatctt ctttaagcaa cgaatgcgtg tcatataaat attgttttgc
     3241 cttctccaac tcttcaggtg catatttgtc tgcttttact tcttcagctt cggttattcc
     3301 atagcgggct ttggacatat ccctgatggg caggtcagaa gcacatccaa tagaaaatac
     3361 tatgcaggat acaagtatgt atatatagat ttgtttcatg gcgactgtct cctta
//
LOCUS       DAORYL010000163         3366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_18819_length_3366_cov_2.832679, whole genome shotgun sequence.
ACCESSION   DAORYL010000163 DAORYL010000000
VERSION     DAORYL010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18819_length_3366_cov_2.832679"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLV81_15215"
     CDS             <1..250
                     /locus_tag="PLV81_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11489.1"
                     /translation="SQNIMPLAVGKLTIKNNSIIEYAICGVRAQKFYNPAVNLHRGGI
                     IVATTGATFKNCIMDVEFLPYVNLYNGKNYGNRSYFTE"
     gene            256..2364
                     /locus_tag="PLV81_15220"
     CDS             256..2364
                     /locus_tag="PLV81_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOM11490.1"
                     /translation="MNDNNYIFSNTPQHVYIDGCTGISFLGCQFNNHNSVVMFNKKSM
                     GIKSMNSNFIVNNGTSFNNLYRAIDARSTGVPISFTVANSTFTNNQTGIYTSYVNNFN
                     LLLNTFNVGGNQMTGATVQLGIQNMYGTGFTIEENHFNKSYNPAYNPSKFGIASYQTG
                     TSSNQIYKNTFNEVNFGNYAWGINRSSTNPNFQGLQYLCNENTQNVNYDFYIYTSGET
                     TWDGIRLNQGSLQSPARNTFSVGGVNQGNDIYNFSPAQLSYYYQTGNTQQTPVSTYKV
                     TTIPISGSETCPSNLCDPPCALRPLDEVELSQLYMEYDSAETAYLNLLYTYNTLMDGG
                     STNNLLTQIQQTWSTEATTLRDELLLLSPYVSQEVLRDVAGTGILPPAMLLVVCMANP
                     DATRSEDFLDYLQYDIPSPLPSYMIAAIETTWDVGTSRTVMENTLGDYNEKMALVSNK
                     ILSDLNYKNNQTIEESNPGDTTNISQQINYWLNRIQTLEAKYEMAEHYFEEGSYALAE
                     DVLLSIPLCYRLTEAEQQTLTSYNYIYQYRKNLLDSGKTLSMLDSSQINELILFANNN
                     ANIAGSIAENALCFYYDICTQDNYDTTGTGNNRQMRNTNIPAAHFQEVQDVVACRVMV
                     LPNPATDKATFQYSINSLKDEPEFEIRDMTGREIMKVRITEKEGVIEWQTVVVKSGMY
                     YFSIKAGSKTLARGKLTVRK"
     gene            2504..>3366
                     /locus_tag="PLV81_15225"
     CDS             2504..>3366
                     /locus_tag="PLV81_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11491.1"
                     /translation="MQVVLILFIILLNTIKVQAQNTFEKIIDTLGSAGAYSIQETFDG
                     GYVFCGGSIYNGNDVCIVKLDSAGTIEWAKIYSGPSMEGANYIEQTPDSGYMVNAVYD
                     IGVPFARSWLLRLDANGDTLWTKVLSGGTGATIPSMVNSMASVNNAIYGLTGYFKQDT
                     MPIVPRVFFIAVLSNGLVAANKTYNYSSTLSSDARAIDKTYDNGFIMAGAGATSSQYS
                     DIYVIRTNGYGDTLWTRTYDKSTSDAAMDVKQTTDSGFIIAAITYDTTVYKNNIYLIK
                     TDAAGDTLWTK"
BASE COUNT         1179 a          591 c          688 g          908 t
ORIGIN      
        1 aagtcagaat ataatgccgc ttgcagtggg taagctcacc attaaaaata attcaataat
       61 agaatatgca atatgtggtg ttcgtgcgca aaagttttat aaccctgccg ttaatttaca
      121 cagaggcgga attattgttg ccactaccgg agctacattt aaaaactgca ttatggatgt
      181 ggagtttttg ccttatgtaa atttatataa tggcaagaat tatggcaaca ggagttattt
      241 tacggagtaa attttatgaa cgataataac tatatttttt caaatacacc gcagcatgta
      301 tatatagatg gttgcacagg cataagtttt ttgggttgtc aattcaacaa tcataattca
      361 gtggtaatgt ttaacaaaaa aagcatggga atcaaatcca tgaatagtaa tttcatagtt
      421 aacaatggta catcgtttaa taacttatat agggccattg atgcacgaag taccggagta
      481 cccatttcct ttactgtggc aaacagcact tttaccaata atcaaacggg gatatatacc
      541 agctacgtca ataatttcaa tctgttgtta aacacattta atgtaggtgg aaatcaaatg
      601 accggagcca cagtacagtt aggcatacaa aacatgtatg gtacaggctt cactattgag
      661 gagaatcatt tcaacaagtc atacaaccca gcatacaatc cttcaaaatt tggtatagcc
      721 agctaccaaa ccggcacatc gagtaatcag atatataaaa acactttcaa cgaagttaac
      781 tttggaaact atgcatgggg catcaaccgc agcagtacaa atccaaactt tcagggactt
      841 caatacttat gtaatgagaa tactcaaaac gtaaattatg atttttatat atatacctca
      901 ggcgagacaa cgtgggatgg catcaggttg aatcagggca gcttgcaaag tcctgcgcga
      961 aacacttttt ctgtaggcgg ggtaaatcaa gggaatgata tttacaattt ttcaccagca
     1021 cagctaagtt attattacca aaccggcaat acacagcaga ctccggtaag tacgtataag
     1081 gtaacaacaa ttccaataag cggaagtgaa acatgcccat ccaatctgtg cgatccacct
     1141 tgtgcattga ggcccttaga tgaggtagag ctatcacagt tgtatatgga atatgattct
     1201 gccgagacgg cctatttgaa tttactctat acatataata cgcttatgga tggtggcagt
     1261 acaaacaatc tgctgacaca gatacagcaa acatggtcca ccgaggcaac aacattacga
     1321 gacgagttgt tgttattgtc accttatgta tcgcaagagg ttttgcgtga tgttgcaggc
     1381 acaggcatac tgccaccagc catgctattg gtagtatgta tggccaatcc tgatgcaaca
     1441 agaagcgaag attttttaga ctatttgcaa tatgatatac catcacccct gccatcatat
     1501 atgatagccg ccattgagac aacgtgggat gtaggcacct cgcgcacagt aatggaaaac
     1561 acactaggcg actacaatga aaaaatggca ttggtgtcaa acaagatatt atcagattta
     1621 aattataaga acaaccaaac tattgaagaa tcgaatccgg gcgacaccac caatatttca
     1681 caacaaataa attattggtt aaatcggatt caaactctgg aggcaaaata tgagatggca
     1741 gaacattact ttgaagaggg cagctatgca ttggcagaag atgttttact atccatacca
     1801 ctatgctacc gattgacaga agcagaacaa caaaccttaa cgagttacaa ctacatttat
     1861 caatacagaa agaatctttt agattctgga aaaaccttgt ccatgctgga tagcagtcag
     1921 atcaatgagt tgattttatt tgcaaataac aacgcaaata tagccggaag catagctgaa
     1981 aacgcactat gtttttatta tgatatatgt acacaggata attatgatac cacaggtaca
     2041 ggaaacaaca gacaaatgcg aaatacaaat attcctgcag cgcattttca ggaggtgcaa
     2101 gatgtagtgg catgcagggt gatggtttta ccgaatccag caactgacaa ggcaacattt
     2161 caatatagca tcaacagctt aaaagatgaa ccagagtttg agataagaga tatgacagga
     2221 cgagaaataa tgaaggtgag gataacagaa aaagaaggtg taatagaatg gcagacagtt
     2281 gttgttaaaa gcggtatgta ttattttagt ataaaagcag gtagtaaaac tttggcaaga
     2341 gggaagttga cggtgagaaa ataaaaagat agcatcctca taggagagtg aaacagaagt
     2401 cattctccta tgaggataaa actgtaaaga aataaaaaag tgtagttaca acagcatagc
     2461 attaatcaat caactaaaaa tgagtaaaaa acaacgaaga aacatgcaag tagtcctcat
     2521 tctatttatt attttgttga acaccataaa agtacaagcc caaaacactt ttgaaaaaat
     2581 tattgatacg ttgggtagtg ccggggcata tagcattcag gaaacctttg atgggggata
     2641 tgttttttgc ggaggcagca tctacaatgg caacgatgtt tgtatagtaa aactcgattc
     2701 tgccggcacc attgaatggg caaagattta cagtgggccc agtatggaag gggctaatta
     2761 tattgagcaa actccggata gtggttatat ggtaaatgca gtttatgata taggcgtacc
     2821 ttttgcaaga agctggcttc taagacttga tgcaaatggt gatacgcttt ggacaaaagt
     2881 tttaagtggt ggtactggag caaccatacc ctcaatggtt aacagcatgg cttcggttaa
     2941 taatgctata tatggactaa cgggttactt taaacaagat acgatgccaa ttgttcccag
     3001 agtttttttt attgccgtac tgagcaacgg attggtggcg gcaaataaaa catataatta
     3061 ttcttctaca ttaagcagcg atgctagagc catagataaa acctatgata acggatttat
     3121 tatggcaggt gcaggtgcca catcatccca gtatagtgat atttatgtaa tccgcaccaa
     3181 tggctatggc gataccttat ggacaagaac ctatgataaa tcaacaagcg atgctgccat
     3241 ggatgtgaaa caaactactg acagtggatt tattattgcg gcaattacct atgatactac
     3301 tgtgtataag aataatattt atttaatcaa aacagatgct gcaggtgata ccctttggac
     3361 aaaaaa
//
LOCUS       DAORYL010000164         3315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_19174_length_3315_cov_7.857669, whole genome shotgun sequence.
ACCESSION   DAORYL010000164 DAORYL010000000
VERSION     DAORYL010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19174_length_3315_cov_7.857669"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3284)
                     /locus_tag="PLV81_15230"
     CDS             complement(<1..3284)
                     /locus_tag="PLV81_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11492.1"
                     /translation="MEIIPQPQGSWDTSDKPDYSPIYDPENGESVLEYLTKKLYNLKL
                     NCNIDLDNYYIEDSLKRMVMYNAYGQLRSSAAFKVSYLDHLLFTLIASYDFGYKTRLE
                     TDIDNRSDEPYINAGGYPDYCYEYFHWHGEPTGGILTVNDCLYSLTSGEKNAIVADVG
                     AYELSLDPTLPKEAGKAYYTASGRTTQCNFVFRSSYNFDSEHATDYKFPMGYDFPSLA
                     LLSGFSAGDAGIPNGGKAGILPTQDLTGNDESTNDYRTYYPYVGNGLGELNTGRWVMG
                     WIARACWEGEGPYYYDPEKAGQTVPTVSINGTTYKVYFRPDGRIYALKNTEDGSFFYP
                     VDGGNDVKDPSNTQLGNYYLRENRYKAVWETDKIMLKAEWANSDRLSQPLFYTLNRVV
                     GNSGLDKYRLRKINGPENTTDDEDAGLESGLSAGGLIFREKIAEDNPVRACSSQEEAL
                     FRNFQWMMLEKKIVFMMPMRSYITVLDLNGDVTLDPLVVTVLEANGIIGMKNLKKGNG
                     VGYWNIKGNEGTDLQGCSGKDKYGNDVNYGDSYELGDGRIFVLVREDAGYLLGLAGSY
                     VDLDEIWNTILGNGAVTPPILAHNLDPIARMAFLQPSLVASNSTEIGNEGSTTWKNRS
                     KLLPIVIALAGDLHDKSFYEIPENAYNPVTNPSGNAWYNFSGKHKYPLKHLRDLIAML
                     DSPMLKYYKVPYPGASKGWFVPQINNPVDAGKFAFLLPQPIADQYYPDIDFRPNQNLR
                     SVIQVLTESGANATAKADGLLPAITNTKLVTRLLSLLQKMGKDEGIYQDEDKTSNDYT
                     KWGARRKFFYGLEQIVTRIKCSQSIELQRGYYNVSYPDWMFTKDDNDLDLDKALEELI
                     GYDAAGSDQWGKGLAAFVDHRDSTHPNYKGYSWKNFYKMINGVGELLSNNGPLTNGNF
                     CITEDLISMLDKTLTSVTATNEQLKALRHTLGVTMTYYDADLNAWVNAPELSAILFNK
                     LPQLLNAYKGNNLNLLIAGLNMMQDDGFVEYMLTTMKTDYPSKQVFEELYAFLGSDLV
                     ANPDSRLWNDVEELITQFAVMLDNSSSKSYRATTFDEYINPVYSNSSEYLYSNEFDPY
                     TGLGMI"
BASE COUNT          971 a          745 c          515 g         1084 t
ORIGIN      
        1 aatatcatcc ctaacccggt atatgggtca aactcattac tgtacagata ctcagaagaa
       61 tttgaataaa caggattaat gtattcatca aatgtggttg ccctgtatga cttactactg
      121 ctgttatcaa gcataacagc aaactgggtt ataagctctt caacatcatt ccataatcgc
      181 gaatcagggt ttgcaaccag gtcgcttcct aaaaaggcgt ataactcttc aaaaacctgc
      241 tttgacgggt aatcagtttt cattgttgtc agcatgtatt caacaaaccc atcatcctgc
      301 atcatattaa gtccagcaat taaaagattt aaattgttac ctttgtaggc attgagcaac
      361 tgtggtaatt tattaaacaa tatagccgat agttccggtg cattgaccca tgcattcaaa
      421 tcagcatcat aataggtcat tgttacaccc agtgtatggc gtaatgcttt caactgctca
      481 tttgttgctg ttacacttgt caatgtttta tcaagcatag aaattaaatc ttcagttata
      541 cagaaattcc catttgttaa tggcccgtta ttgctcagca gttcgcctac accattaatc
      601 atcttataga aatttttcca gctatatcct ttgtaatttg ggtgagtact atccctgtga
      661 tcaacaaatg ctgccaatcc tttaccccac tggtcgctgc ctgctgcatc atagcctatc
      721 agttcttcca gtgctttatc taaatccagg tcattatcat ctttggtaaa catccagtcg
      781 gggtagctga cgttataata tccacgctgc aactctatac tttgactgca ttttatcctg
      841 gttactatct gctctaaccc ataaaagaat tttctgcgtg ctccccattt tgtgtaatcg
      901 ttgcttgttt tatcttcatc ctggtatatt ccttcatctt ttcccatttt ttgcaaaagc
      961 gatagtaacc gggtaacaag cttagtattt gtaatagctg gcagcaggcc atcagccttt
     1021 gcagttgcgt ttgcaccgct ttcagtcaat acctgaatga ctgaacgaag gttttgattt
     1081 ggtctgaaat caatatcagg ataatactgg tcagcaattg gctgtggcaa aagaaatgca
     1141 aatttccctg catccactgg attattaatt tgtggtacaa accatccttt tgaggcaccc
     1201 ggatacggta ctttataata cttaagcata gggctatcaa gcatagcaat gagatcacgt
     1261 agatgtttta atggatattt atgtttccca ctaaaattat accaggcatt gccgcttgga
     1321 tttgttaccg gattatatgc attttccggt atttcataaa atgacttatc atgtaaatca
     1381 cctgctaatg ctataacgat aggtaataat ttgctgcggt ttttccatgt tgttgaacct
     1441 tcattgccaa tttcagttga atttgatgct accaatgaag gttgcaaaaa cgccattcgc
     1501 gcaattggat ctaaattatg ggcaagtata ggcggagtta cagcaccatt acctaatatt
     1561 gtattccata tttcatcaag atccacatag ctaccagcta aacccaataa atatcctgcg
     1621 tcttctcgta caagcacaaa tattcttcca tcacctaatt catacgaatc accgtaattg
     1681 acatcattcc catatttatc tttaccacta caaccttgta aatctgtccc ttcattgccc
     1741 tttatattcc agtacccaac accatttcct ttctttaaat ttttcatccc aataatacca
     1801 tttgcttcta aaacagttac caccaaagga tcaagtgtta catctccgtt taaatctagt
     1861 acagtaatat acgaacgcat tggcatcata aagactatct ttttttcaag catcatccat
     1921 tgaaaattcc tgaataatgc ttcttcctgg ctactacacg cccttacagg attatcctca
     1981 gctatttttt ctctaaaaat taaaccacca gcagacaaac cactttctaa accggcatct
     2041 tcatcatcag ttgtattttc aggcccattt atttttcgta atctatattt atccagtcct
     2101 gaatttccaa ctacacggtt taatgtataa aataatggtt gggataatct atcactattt
     2161 gcccattcag ctttgagcat tattttatct gtttcccata ccgctttata tctattttca
     2221 cgcaaataat aattccctaa ctgtgtattt gacggatcct taacgtcatt cccaccatct
     2281 acaggataaa agaaactacc atcttcagta ttttttaatg catatatcct gccatcaggc
     2341 ctaaagtaaa ctttatatgt tgtaccattg atactaactg tgggaactgt ctgccctgcc
     2401 ttttcgggat cataatagta tgggccctca ccttcccagc aagcacgggc tatccagccc
     2461 attacccagc gcccggtgtt aagttcaccc aatccattac ccacatacgg ataatatgtg
     2521 cgatagtcat tggttgattc atcattgcct gttaaatcct gtgttggcaa tatccctgct
     2581 ttcccaccat ttggtatacc agcatcacct gccgaaaacc ctgaaagcaa tgccagcgat
     2641 ggaaaatcgt atcccatagg aaacttataa tcagttgcat gctcactatc aaaattgtat
     2701 gaacttctaa atacaaagtt gcattgcgtt gtacggccac tggctgtata atacgcttta
     2761 ccggcctctt ttggaagagt tgggtcaagc gatagctcat aagcaccaac atctgctaca
     2821 atagcatttt tttcaccaga tgttaacgaa tagagacaat cattgacagt aaggatacca
     2881 ccggtaggtt cgccatgcca gtggaaatac tcataacaat aatcaggata tcctcctgca
     2941 tttatataag gctcatcgct tctattatct atatctgttt caaggcgggt cttgtatcca
     3001 aaatcgtatg aagcaattaa tgtgaataac aaatgatcca gatatgaaac cttaaaagca
     3061 gcactgcttc ttagttggcc atacgcatta tacataacca tccgctttaa tgaatcttca
     3121 atgtaataat tatctaaatc tatattgcaa tttaatttta aattatataa tttctttgtt
     3181 aaatattcta atactgactc cccattctca ggatcataaa tgggcgaata atcaggttta
     3241 tcactggtat cccaactacc ttgaggctgg ggaataattt ccaaaaacag ctcatccaat
     3301 tctccatatt cgata
//
LOCUS       DAORYL010000165         3203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_20050_length_3203_cov_13.389454, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000165 DAORYL010000000
VERSION     DAORYL010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20050_length_3203_cov_13.389454"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /gene="dusB"
                     /locus_tag="PLV81_15235"
     CDS             complement(<1..855)
                     /gene="dusB"
                     /locus_tag="PLV81_15235"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOM11493.1"
                     /translation="MDFPIQLQNPIMLAPMAGYTDSPFRRIARSFGAGMVFTELISVD
                     GIVRHNKKTLDLLTFTPYERPLGIQIFGNDPAIMQEAACQLLSLKPDCIDINCGCCAP
                     KVCNNGKGAGLLKDVDLLYKIACAVVSSIHVPVSAKIRLGFSQGTKNYMEVVHALEEA
                     GVAFITVHGRTRDQYYSGKADWEAITHIARTAKVPIVGNGDITSYDDALRKLQSSGCS
                     AVMIGRAALGNPWIFCGKTPSWEEKRETIALHYKLMIEHYGSYGVILMRKHIVHYLKG
                     FPGASKLRQ"
     gene            1305..>3203
                     /locus_tag="PLV81_15240"
     CDS             1305..>3203
                     /locus_tag="PLV81_15240"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOM11494.1"
                     /translation="MKKYIVLCCSIILCAMPLFAQDFTIIQTTDLHSHFLGFSPNIDY
                     TPNTINDDATVGGFARIATVINDIKKSRKNPVLLLDSGDFLMGSVFHLLCREEAFELR
                     LMKKMGYDVITLGNHEFDLYPDGLARIIRAAQKYNALPAIVASNIVFSADSKDDDSLE
                     KVFEDELVKPYTVITKEKLKIGFFGLIGKDAAEVSPFAHPVTFADQVQTAKQMVDILR
                     NKEKVDVVICISHSGIRKYQDKDEIEPGRSEDVQLAKEVKGIDVIVSGHTHVKIKQPI
                     IVGKTIITQSYEYGKQVAVLDLSLSKDGVVLKNYTYVDINDSIKGDPAITALINQFVQ
                     RINQQVLSPLKLTCDSVLAKTKFDLVIKEEETNLGNLIADSIRWYVNSVDFDPKDPDS
                     RVAIGVISNGVIRDNIVVGKTGKVVLTDAFATIPLGIGMDEKKTMGYPLITCYVYASE
                     IKKALEILTSIYPLKGSDYFIQVSGVKFTYNPNRMLFDRVTEIWIGDEQNGYVPLDYS
                     DNNKKLYRIAADIYNATFLKIIGNFTWHILDIVPKWRDGKPIEKLTEARVDTDKRKPG
                     IQEAKEWIGVIEYIRSFKDEDGDGTPDIPAMYQGKLGRIVVEASLNPYHLLKRGTKVT
                     WIGFIIFLI"
BASE COUNT          992 a          628 c          669 g          914 t
ORIGIN      
        1 ctgccttagt ttgcttgcac caggaaaccc tttaaggtaa tgcacaatat gcttgcgcat
       61 aagtatgaca ccgtaagagc catagtgttc tatcataagt ttgtagtgta atgcaattgt
      121 ttcacgcttc tcttcccacg agggagtttt accgcaaaat atccagggat tacccaatgc
      181 agcgcgtcca atcatcactg cgctgcaacc gcttgattgt aatttcctca gggcatcatc
      241 atagcttgtg atgtcgccat taccaacaat gggaactttt gcggtacgtg caatatgggt
      301 tattgcttcc cagtccgctt taccactgta gtactggtca cgcgttctgc catgcactgt
      361 aatgaaggca acaccagctt cttcaagcgc atgcacaacc tccatataat tttttgtgcc
      421 ctgcgaaaac ccaagtcgta tctttgctga aactggcaca tgtatgctgg acactacagc
      481 gcaagctatt ttatacagta aatcaacatc tttcagtaaa ccagcacctt tgccgttgtt
      541 acacacttta ggagcgcaac aaccgcagtt aatgtcaata caatcaggtt taagcgatag
      601 taactggcaa gccgcttcct gcataatagc tgggtcatta ccaaaaatct gaatccctaa
      661 tggcctttca tatggcgtaa atgtaagcag atcaagagtc tttttattgt gccgaacaat
      721 gccatcgacg ctgataagct cagtgaaaac catccccgcg ccaaaagagc gagcaatacg
      781 cctgaacggc gagtcggtgt aaccagccat tggcgcaagc atgataggat tttgtaattg
      841 aattggaaag tccatagcta aaaaaaccat agcacaacag gttacactgt taaaattaaa
      901 ttttcttcac gccgggatat atagctctga ccccatttta ccagagtacc ctgcatgata
      961 gaaaaaatgg ctcatgcaaa gatgctgata acacagggta caaagatttt ttagataaat
     1021 tgctctgacc ccttactgta gcgtagtcag cgattttcgt gcacactaca agagagggaa
     1081 attccacact cagagatgca aagactccag tggttgtatt atgatgctct gacccccgtt
     1141 aatcagcgca cgtagcgatc tctgtgcact tagcgtgatt atccctttaa tcagtattga
     1201 tgataaattt tatttatcat caatatttta tacttgataa aaaattgtta ctgcaactac
     1261 aataactgac actatacgta ataccataca gggggtttgg taccatgaaa aaatatatag
     1321 tactttgctg cagtatcata ctttgtgcaa tgccattatt tgcacaggat tttactataa
     1381 ttcagacaac tgacctgcat tcacattttt tagggttttc acccaatatt gattatacac
     1441 ctaatacaat aaatgatgat gctaccgttg gtggttttgc acgtattgct actgtcatca
     1501 atgacattaa gaaaagcaga aaaaatcctg tactgctgct tgattctggc gattttttaa
     1561 tgggatcggt gtttcatctt ttgtgccgtg aagaagcctt tgaactgcgc ctcatgaaaa
     1621 aaatggggta tgacgtcatt acactgggta atcatgaatt tgatctgtat cccgatggcc
     1681 tggcgcgcat catacgggca gcacagaagt acaatgcatt gcctgcaatt gttgcaagca
     1741 atattgtatt cagtgccgat agcaaggatg atgatagcct tgaaaaagtc tttgaagacg
     1801 agttggtcaa accatatact gtgataacaa aagaaaaatt aaagataggc ttttttgggc
     1861 ttattgggaa agatgctgcg gaagtttcac catttgctca tcctgtaacc tttgctgatc
     1921 aggtacaaac agcaaaacag atggtggata tattgcgaaa caaagaaaag gttgatgtag
     1981 taatatgcat ttcgcacagt ggcatcagaa agtatcagga taaagatgaa attgaacctg
     2041 gacgttccga agatgtgcag ctggcaaaag aagtcaaagg aattgacgtt atcgtcagtg
     2101 ggcatactca tgtaaaaata aaacagccaa ttattgttgg caaaacaatc attacacagt
     2161 cgtatgagta tggcaagcag gttgctgttc ttgacttgag tttatcaaaa gatggtgtcg
     2221 tattaaaaaa ctatacatat gttgatatca atgattctat aaaaggggat ccagcaataa
     2281 ctgctctcat caatcagttt gtgcaaagga ttaatcaaca ggtactatcg cccctgaaac
     2341 ttacatgcga tagtgtgctt gccaaaacaa aatttgattt agtcatcaaa gaggaagaaa
     2401 ctaacctggg caaccttatt gctgattcaa tacgctggta tgttaacagc gttgattttg
     2461 atccaaagga tcctgatagc agggtagcaa taggtgtaat atctaacggt gtcattcgcg
     2521 acaacattgt ggtgggtaaa acaggtaaag tggtgctgac cgatgcattt gctactattc
     2581 cgcttggcat tggcatggat gaaaaaaaga ctatgggcta tccactgata acctgctatg
     2641 tgtatgcgtc ggaaattaag aaggcacttg aaattttaac aagcatctat ccactaaaag
     2701 ggagcgatta ttttattcag gtttcaggtg tcaaatttac ctataatccc aaccgaatgc
     2761 tttttgatcg cgtgacggaa atctggattg gcgatgaaca aaacggctat gtgccacttg
     2821 attattcgga taataataaa aagttatatc gtattgctgc tgatatctat aacgcaacgt
     2881 ttttaaaaat cattggtaac ttcacctggc atattctgga cattgtaccc aaatggcgtg
     2941 atgggaaacc aatagaaaag cttaccgaag cacgtgttga tactgataaa cgaaaacctg
     3001 gcatacagga agcaaaggaa tggataggtg ttattgaata tatccgtagc ttcaaggatg
     3061 aggacggtga tggcactcct gatattcctg ctatgtatca ggggaagctt gggcgtatag
     3121 ttgttgaagc aagcttaaat ccatatcatt tactcaagcg cggaacaaag gttacctgga
     3181 ttggatttat aatcttttta ata
//
LOCUS       DAORYL010000166         3158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_20440_length_3158_cov_9.193361, whole genome shotgun sequence.
ACCESSION   DAORYL010000166 DAORYL010000000
VERSION     DAORYL010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20440_length_3158_cov_9.193361"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..2952
                     /locus_tag="PLV81_15245"
     CDS             283..2952
                     /locus_tag="PLV81_15245"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOM11495.1"
                     /translation="MKKWFNIVLTYPKTVIVIIIVITVLLGSGVFKIQFDSSLDAVMP
                     RHDPEYLLNEEVKKTYGNTGKFIIIDVASSDMLQPHILTLVSNLHDDIDEYRRFDEGR
                     EMHRLSLLQELSHNKNITKQMLLQTFADDPVFTRYIMQTLQQLDIEGDRLSQRDMTKI
                     IKKFTRTIEVKRNETVDLIISPFTMKDLRGQNDTLYVRDIVEKDETGKRKVPQTAEEI
                     AEFKKRLTNNPAFEKGIYVKDAQGTITDFGILLRLVDQPQYDPLVKEILDIISYYQGA
                     ADIVIQGVPVTHHEINEYMKTDLERFLPLVMLVILIVFYLNFRSLRGMILPFATLILA
                     DLWVIGLMGHLGFKLNVIGVALPPLMTAVGSSYSIHILNQYYLEQNNINRLGKFKGIR
                     LALTHIALTVILAGLTTFLGFITLATNQVSSIREMGIFSAIGVLFAVLISLTIIPAEL
                     MLLPQEGKTITYKSKIISFFSNLNVVENIVKKLAHYAVENSLTVITALVLALGVAFVG
                     LINVKVESSVHAYFKEGDYVLTSSKIIGQKFGGAYGLNILIDTGKIDGVKDPQFLTFI
                     DSFREWLIADENIDLNIGRTDAITDFIKTINLAMHDNDFAYYCIPETQKDVDSYIDLY
                     GGKDDNDNGLADEFEPYVDRKFTTVNIFARQWEKEGKLLSSHMMHNIIRKISMYLDHN
                     LPEGYTYKISGEPKVLVRMSDYIVQGQIMSLALSLVSIFMIVLLLFRNIKAACVSLIP
                     ISTAVLLNFGVMGFLGIRLDIATAIIASITIGIGIDDTIHFLNTYRHFHLQGLSQKDV
                     ILHTLNRAGVAIVYTSLALIFGFLVLVISSFKPVIYFSILLATTMITTTLGALLFLPA
                     TINLLRLDLSPSKSTSKVWQYFNIGKYFELESE"
BASE COUNT         1016 a          567 c          600 g          975 t
ORIGIN      
        1 ccgcattaca tgaatcaata ccggcaacgc aagatgaaca gcttttcatt gataacgtat
       61 gggtactggc tgtactggat agcaacggta acggaaaacc agatagtggg gaatatattg
      121 gttactattg gaagagagat attacgacac tatggtcata tgtaccaata aaactacctt
      181 tacctttggg tgatggtact tcgattttga acaagacagt gcgtttttca agttatacat
      241 attaagttgc ataatcaata ataacaataa aggaatcggc ttatgaaaaa gtggtttaac
      301 atagtgctta cctaccccaa aacagtgata gtaattatca tcgtcatcac cgtactatta
      361 ggcagtggcg tatttaagat tcagtttgat agctctcttg atgctgtcat gcccagacat
      421 gatcctgaat atttactgaa tgaagaagta aaaaagacct atggcaatac cggcaaattt
      481 atcatcatag atgttgcatc aagtgacatg ctgcaaccac acattttaac actggtatcc
      541 aatcttcacg atgatataga tgaataccgg cgatttgatg aaggcaggga aatgcaccgg
      601 ctttctcttc tacaggaact atcgcacaac aaaaacatta ctaaacaaat gctgttacag
      661 acatttgcag atgatcctgt ttttacacgc tacattatgc aaaccttaca acagcttgac
      721 attgaaggtg acaggctttc acaacgcgat atgacaaaga ttattaaaaa atttaccaga
      781 accattgaag tcaaacgcaa tgaaaccgtt gatttaatca tttcaccgtt taccatgaaa
      841 gaccttcgcg gccaaaacga taccctgtat gtacgcgata tagtggaaaa agatgaaaca
      901 ggcaaacgaa aagtcccgca aactgctgaa gaaattgctg aatttaaaaa gcggcttacc
      961 aataaccctg catttgaaaa aggcatttac gttaaggatg ctcaaggaac aatcactgat
     1021 tttgggatac tgcttcgtct tgttgaccag ccacaatatg atcctttagt aaaagagatt
     1081 ctggatatca tcagttacta tcagggtgct gctgacatag ttatacaggg tgtccccgta
     1141 acccatcatg aaattaatga atatatgaaa accgacttag agcggttttt accactggtg
     1201 atgcttgtta ttcttattgt attttatctt aatttcagat cactccgtgg catgatattg
     1261 ccatttgcaa cactgatcct tgctgacctg tgggtaattg gacttatggg acatttagga
     1321 ttcaagctaa acgtgatagg tgttgcactt ccacctttga tgactgctgt gggcagttca
     1381 tactccattc atattttaaa tcagtattac cttgaacaaa ataatattaa caggcttggg
     1441 aaattcaaag gcatacggct tgccctgaca catattgctc ttacggtgat acttgcagga
     1501 ttgaccacat ttttaggatt tattactctt gctacaaatc aggtttcctc aatccgtgaa
     1561 atgggtatct tctctgcaat tggcgtatta tttgccgtgc tcatttcgct tacaataatt
     1621 cctgcagaac tcatgcttct tccacaggaa ggtaaaacaa taacatataa atcaaagatt
     1681 atatcttttt tcagcaatct caatgttgtg gaaaatattg taaaaaaact tgctcactat
     1741 gcagtagaaa acagcctaac tgtcataact gcactggtat tagctttagg cgttgctttt
     1801 gtgggactta ttaatgttaa agttgagtca tcagtacatg cgtactttaa agaaggagac
     1861 tacgtactga caagctcaaa aattattggc cagaagtttg gcggagcgta tggattaaat
     1921 attttgatag atactggtaa gatcgatggt gtaaaagacc cgcaattttt aacattcatc
     1981 gacagcttta gagaatggct tattgctgat gagaatattg acttaaatat tggaaggact
     2041 gatgcaatta ccgattttat taaaacaatt aatttagcca tgcatgacaa tgactttgcc
     2101 tattactgca tccctgaaac acagaaagac gttgacagct atattgacct ttatggtggc
     2161 aaggatgata atgacaatgg tcttgccgat gaatttgagc cctacgttga cagaaaattt
     2221 accactgtaa atatctttgc ccgtcaatgg gaaaaagaag gcaaattgtt aagttctcac
     2281 atgatgcata acattatacg caaaataagc atgtacctgg atcataacct tcctgaaggg
     2341 tatacatata aaatcagtgg agaacccaaa gtattggtac gcatgtcaga ctatatcgtt
     2401 cagggtcaga tcatgagcct ggcgttaagt cttgtgagta tatttatgat agttctttta
     2461 ctttttcgca acatcaaagc agcatgtgtc tcgcttatcc ccataagtac agcagtatta
     2521 ttaaattttg gtgtgatggg atttttaggc atacgacttg atattgcaac tgcaatcatt
     2581 gcatccatta ctattggaat aggtattgat gacaccattc actttttgaa tacatacagg
     2641 catttccatt tacagggact ttcgcaaaaa gatgtaattt tacacacatt aaatcgtgca
     2701 ggagtagcca ttgtatatac atcattggca ctgatatttg gatttctggt actggtaata
     2761 tcaagcttta aacctgtcat ctatttttca atattactgg caactactat gattacaacc
     2821 acgttggggg cattgctgtt tttgcctgca acaattaatc ttttgcggtt agatctatcg
     2881 ccatcaaaaa gcacatcaaa agtatggcaa tatttcaaca ttggtaaata ttttgaactg
     2941 gaatcagaat agtgcaacta tcagctttct tgggattgga aatgatccag attagtattt
     3001 ccattatcat aagaagaaaa attattaaaa taatgaaaca ccaattttta ttaacaatat
     3061 atttttaaca ggagggatac aatgaaaaag tttgtagtat taggtttgtt acctgtgttt
     3121 tgtgtggcga tagttattgt gtatgcaatg acaggcag
//
LOCUS       DAORYL010000167         3099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_20953_length_3099_cov_9.056505, whole genome shotgun sequence.
ACCESSION   DAORYL010000167 DAORYL010000000
VERSION     DAORYL010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20953_length_3099_cov_9.056505"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PLV81_15250"
     CDS             <1..182
                     /locus_tag="PLV81_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11496.1"
                     /translation="RYHYSSIRAYLDEDADVGVPIDHHDYFVQLGKTFAERVVKFMWY
                     EEYYRKKYSMILEWV"
     gene            complement(453..1661)
                     /locus_tag="PLV81_15255"
     CDS             complement(453..1661)
                     /locus_tag="PLV81_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOM11497.1"
                     /translation="MDLFDKCFSFTRVEEVKAVGLYPYYHPITETEGPVVYMNNQRMI
                     MVGSNNYLGLTHDPRVKKAAIDAINKYGTGCSGSRLINGSLDIHCKLEEALAQFVGKE
                     AACIFSTGYQTNQGAIVPLIKKGEYIISDAENHASIIQGTLIAKALCGDSILVKFNHN
                     DMTDLEQKISSLLYEAGKLIVVDGVFSMNGHITPLPQIVAIAHKYNARIMVDDAHALG
                     VIGTGGRGTASHFNLTDETDIIMGTFSKSLASSGGFLASSERVINYIKHHAPAIIFNA
                     SMPASNAAAALKALEIIQKEPERIQKLHYNAHRLRKGLSELGFSVLPGETPIIPVLIG
                     DDFMTFALWKKLFDYGVYSNPIIFPATPQGQQLIRLSVMATHTDEHIDYVIAMFEKAG
                     VELKLLNTVV"
     gene            2022..3071
                     /locus_tag="PLV81_15260"
     CDS             2022..3071
                     /locus_tag="PLV81_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11498.1"
                     /translation="MAQRITKKIMKLAPGIIAIVIVLTGVAVVYLYSDPVMRFMMVMN
                     EVIPAWGVSSEEVVLPLENNSKTKMTIYKRIFSKQDKYYFCVHGLTPQGYQHPSLVKL
                     ARALALATGRKVLVPYLYGSETDRDVIDATQEIKKMYISVKERYPGKYNGFGACISAT
                     MLVVALNDLPTERYPDKIFMYGPFLNGEMLMHFYNTSGMEVDFIVKLANAMRHPKLTE
                     EEKKLVGKAIAATKPGVTDRNQVKQILGIDLYNKVDSLKVENPEFKQLNEMTLFKQKP
                     LPACRYYILHSRADNIIPFSMGLSMHKYLLQLGLDSKFLATGAFQHTQKEKSFTTLKT
                     EFKEIYVFFNDLFKE"
BASE COUNT         1067 a          496 c          604 g          932 t
ORIGIN      
        1 ccagatacca ctacagcagc atacgagcat atttagatga agatgctgat gtaggtgtac
       61 ccatagatca tcatgattac tttgtgcaac tggggaagac gtttgctgag agggtagtaa
      121 agtttatgtg gtatgaggag tattaccgaa agaagtattc catgattctt gagtgggtgt
      181 aaaagaaaag agaaaaaagg aaaaaagggt cggaaaaaag gtcagagccg taacgttcgg
      241 caagattaat gcagtaggag gggtattatc aaaaggagta ttccatgata cctgagtgtg
      301 tatgaaaaat aaaaaggggt cagagccaaa catggaaagt catttaccaa gaagatggta
      361 atattaaagc gatatgagga atgctatcgt aagaaatatt ctatgatact agaattgaaa
      421 gtaagggtca gagcatatat cgagcaaata tcttacacaa ctgtgtttag caattttagc
      481 tcaactccag ctttttcaaa catagcaata acatagtcaa tatgttcatc agtatgagtt
      541 gccataacac taagacgtat taattgctgc ccttgtggtg ttgctggaaa aattattgga
      601 tttgaatata caccatagtc aaaaagtttt ttccataatg caaaagtcat aaaatcatca
      661 ccaattagta caggtattat tggtgtttca ccaggcaaca ctgaaaatcc aagttcagaa
      721 agtcctttac gaaggcgatg ggcattataa tgaagttttt gtattcgttc aggctctttc
      781 tggattatct ccaaagcttt taatgctgcc gcagcatttg aagctggcat acttgcattg
      841 aaaataattg ctggtgcatg gtgtttaata taattgatta ctcgttcgct ggatgctaaa
      901 aagccaccag atgaagccag gctttttgaa aaagtaccca taataatatc tgtttcatca
      961 gtaaggttaa agtggcttgc agttcccctg ccaccagtgc ctatcactcc caatgcgtga
     1021 gcatcgtcta ccataatacg agcattatat ttatgagcta tcgctactat ttgtggtaat
     1081 ggtgtaatgt gcccattcat gctgaagaca ccatcaacaa caattaactt tcccgcttca
     1141 tacagcaggc ttgaaatttt ttgttcaaga tcggtcatat cattatgatt gaactttacc
     1201 aggatactat cgccacataa tgcttttgca attaaagtgc cctgaataat acttgcgtgg
     1261 ttttctgcat cagaaataat atattcccct ttttttatta aaggtacaat agctccctga
     1321 ttggtttgat agcctgtgga aaaaatacat gcagcttctt ttccaacaaa ctgtgctaaa
     1381 gcttcttcta atttacaatg aatatcaagt gatccattga taagccgtga accagaacaa
     1441 cctgtgccat atttgttgat agcgtctatt gcagcctttt taactcttgg atcatgagta
     1501 agccccaaat agttattgga acctaccatg atcatacgtt gattattcat ataaactaca
     1561 ggtccttcag tttcagttat ggggtggtag tatggataca gtccaactgc tttaacctcc
     1621 tcaacacgtg taaaagaaaa gcatttgtca aaaagatcca tggaaaacct ccttaatact
     1681 tttattttaa ttatattttt acattaggtt tttaggtttc atgtatgcat caaaattaga
     1741 taatgtaata aaaatactga ttttaaaata aaaagtcaat atttatgtat aatatgatat
     1801 ataaaaataa attaataaaa aaatgttata taatataaaa ataagagtga acattcactc
     1861 gtaaatgaat aagtactcac tcattttttt atggggtcag agccatgagg tggataaaaa
     1921 ataaaatcag tggggtcaga gcctaacagt aaaaaataat tgcaataaat tttaataact
     1981 atcttttaag caactaggtt tgcatctgag gaggtagaaa tatggcccaa agaataacaa
     2041 aaaaaattat gaaattggct ccaggcatta ttgcaattgt tattgtcctg acaggggtgg
     2101 cagtagttta tttatatagt gaccctgtga tgcgctttat gatggttatg aatgaagtaa
     2161 tcccggcatg gggtgtaagt tcagaggaag ttgtattgcc gttggaaaat aacagtaaaa
     2221 caaaaatgac aatatataag cgaattttca gcaaacagga taagtattat ttttgcgtac
     2281 atggcctgac accgcagggt tatcaacatc catctttggt aaaattggcc agagcgctgg
     2341 cacttgcaac cggcagaaaa gtgctggttc cctatctcta tgggagtgaa accgataggg
     2401 atgtaataga tgcaactcaa gagattaaaa aaatgtatat ttcagttaag gaacgttatc
     2461 ccgggaaata taacggtttt ggagcgtgca tatcggcaac aatgttagtt gtagcactca
     2521 atgatcttcc tacagaacgg tatccagata aaatctttat gtatggcccc tttttaaatg
     2581 gtgaaatgct catgcatttt tacaatacca gtggtatgga ggttgatttc attgtaaagt
     2641 tagccaatgc aatgcgtcat ccaaaactaa cagaagaaga aaaaaagctg gtaggtaaag
     2701 ctatagcagc tacaaaacca ggggttaccg atagaaacca ggtaaaacaa atattgggca
     2761 ttgatctgta taacaaagtg gattcattaa aagtggaaaa tcctgaattt aagcagttaa
     2821 atgaaatgac actatttaaa caaaagcctt taccagcatg caggtattat atattacatt
     2881 cacgtgctga taacattatt cccttcagta tgggcttaag tatgcataag tatctcttac
     2941 aattgggatt ggatagtaaa tttttagcaa cgggagcttt ccagcatact caaaaagaga
     3001 agtcatttac aacactaaaa acagagttta aagagatata tgtttttttt aatgatctct
     3061 ttaaagaata aaaaagtaaa tgggcgatag ttatggtta
//
LOCUS       DAORYL010000168         3095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_20992_length_3095_cov_14.199013, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000168 DAORYL010000000
VERSION     DAORYL010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20992_length_3095_cov_14.199013"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    59..158
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(284..730)
                     /locus_tag="PLV81_15265"
     CDS             complement(284..730)
                     /locus_tag="PLV81_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOM11499.1"
                     /translation="MESRNVLLIFNQHIMYKPIIYRIAKDFNVIFNVLEAKILPKQEG
                     RLILQLSGEKQDLDACITYLKDEEVVVDILADKIQRDEDRCVHCGACTAVCRTDALVI
                     NRETMMVEFHPDRCVACGLCKLACPVKAMSGISIDVDSEELAVKAV"
     gene            complement(743..1465)
                     /locus_tag="PLV81_15270"
     CDS             complement(743..1465)
                     /locus_tag="PLV81_15270"
                     /inference="COORDINATES: protein motif:HMM:NF003323.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HOM11500.1"
                     /translation="MSNTLPYRTKLATRFTTFEVRVETTNLYICAHSNVYDRAIVLVK
                     SLREDIERYCEKHPQFLHALTPLEPEALAPEVVQRMCKASLLAGVGPMAAVAGAIAQM
                     VGEDLLHYSPEVIVENGGDIWAYVREPAKISVFAGRSAFSKHLGIALTPDMTPCGICS
                     SSGRFGRSFSFGKADIATIIADTAALADAVATATCNMVQSDSDCTQAVSFATSISGVK
                     GALVIYWDTMAVKGSIELTEIE"
     gene            complement(1458..2648)
                     /locus_tag="PLV81_15275"
     CDS             complement(1458..2648)
                     /locus_tag="PLV81_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HOM11501.1"
                     /translation="MSDIVIKKSYEEINAKIKKGDAVVVTAEEMAGIVKSEGYKSAFK
                     KVDVVTTGTFGTMCSSGAFINFGHTRPKIKASKVWLNDVEAYAGLAAVDIYIGATQVQ
                     EDDPLNKVHPGRFKYGGGHVIEDLIAGKPVKLKAKSYGTDCYPLREYEATVTIHDLRD
                     AFLYNPRNAYQNYNCAVNQSKKTIYTYMGILRPNMGNATYSSAGQLSPLLNDPYYWTI
                     GVGTKIFLGGTVGAVTWRGTQHDPNAPRGDNGVVRSGAGTIAVTGDMKQMSAQFIKGA
                     SITGYGCSLMVGLGIPIPMLNEDLAFFTGVSDDQIFCQVVDYGYDYPNAIGRVIAEVS
                     YAQLKSGFIEIGGKRIPTAPLSSYPMAKKIATILKDWIKASQFTLGQPQILLPSVPYD
                     KRHE"
BASE COUNT          843 a          678 c          628 g          846 t
ORIGIN      
        1 tacacccgtc cctacacccg tccctacacc cgtccctaca cccgtcccta cacccgtcnn
       61 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc tacacccatt tgcatatccg
      181 ttaacaacac cgttttcata cacccgataa caacaccatt ccacgaaccg tccctacacc
      241 cgtgtgcata tccgttcaat ataacaatcg tataaaaccg tacctacacg gcttttactg
      301 ctaattcctc tgaatccaca tctatggata tgccgctcat tgccttgacg gggcaggcca
      361 gtttgcataa accgcatgct acacaacggt cgggatgaaa ttccaccatc atggtttcac
      421 ggtttataac aagggcatcg gtgcggcata cagcggtgca ggcgccacag tgcacacagc
      481 gatcttcgtc acgttgaatt ttatcggcaa ggatgtctac aacaacttcc tcatctttaa
      541 ggtaggttat gcaggcatcc aggtcctgtt tttcgcctga tagctgcaat ataagccttc
      601 cttcctgctt gggtaatatt ttggcttcaa gcacgttgaa gattacatta aaatcttttg
      661 caatacgata tatgattggt ttgtacatga tgtgctgatt aaaaattaac agtacatttc
      721 gtgattccat ggtaaacctc gtttattcaa tttcagtaag ttcaatgcta cctttgactg
      781 ccattgtatc ccagtatatc accagtgcgc cttttactcc agatatactg gttgcaaacg
      841 atacagcttg tgtgcagtca ctatcgctct gtaccatgtt gcatgtggcg gtggctacgg
      901 catcggccag tgcggcagta tcagcaatga ttgtggcaat atcggctttg ccaaagctaa
      961 atgagcggcc aaacctgccc gatgatgaac agatgccaca gggcgtcata tcaggggtca
     1021 gagcaatacc taaatgtttt gaaaacgctg agcgccccgc aaaaacgcta attttagctg
     1081 gttcacgcac ataggcccat atatcgccgc cgttttcaac aatgacctca gggctatagt
     1141 gcagcaagtc ttctcccacc atctgggcga tagcgcccgc gactgctgcc atgggaccaa
     1201 ctcctgccag caacgaagct ttgcacatgc gttgcacaac ctcaggcgct aatgcctcag
     1261 gctcaagcgg ggtcagagca tgaagaaact gtggatgttt ttcacaatag cgctcaatat
     1321 cttcccgtaa gcttttgaca agaactatcg ccctatcata tacgttggaa tgagcgcata
     1381 tataaagatt ggttgtttca acacgtacct caaaggtggt gaaccgtgtg gcaagctttg
     1441 ttcggtaggg caatgtgtta ctcatggcgt ttatcatacg gtaccgatgg cagaagtatt
     1501 tgtggctgac ctaaggtaaa ctggcttgct tttatccaat ctttaagaat tgtagcaatc
     1561 ttttttgcca tggggtagct tgaaagtggt gcggttggaa tacgtttgcc gccaatttca
     1621 ataaaccctg attttagctg tgcgtaactt acctcggcta tgactcgtcc tattgcgttg
     1681 gggtaatcat agccgtagtc cacaacctgg cagaagatct ggtcatcgga cacgccggta
     1741 aagaatgcca ggtcctcgtt taacatggga ataggaatcc caagtccaac cataagcgag
     1801 cagccatacc cggtaattga tgcaccttta atgaattgcg ctgacatctg tttcatatcg
     1861 ccggttacag caatggtgcc tgcacctgat cgcaccacac cattatcgcc gcggggtgca
     1921 ttggggtcat gctgagtacc gcgccatgtt acagcaccaa cagtaccgcc taaaaatatc
     1981 ttggtaccta caccaattgt ccagtaatac gggtcattga gcagggggct tagctggccg
     2041 gcacttgagt aagttgcatt ccccatattg gggcgcagta tccccatgta cgtgtagata
     2101 gtttttttgg actggttaac tgcacagtta tagttttgat aggcattgcg gggattgtat
     2161 aaaaatgcat cacgcaggtc atgtatggtt actgttgctt catattcgcg taagggataa
     2221 caatctgtac catagctttt tgctttaagc tttacgggtt tgccggcaat taaatcctca
     2281 atgacatgtc caccaccata tttaaagcgt cctggatgta ctttattgag tgggtcatct
     2341 tcctgtacct gtgttgcgcc aatgtaaata tccactgcgg caaggccagc atacgcctca
     2401 acatcgttaa gccacacctt tgatgcttta atcttggggc gggtgtggcc aaaattaata
     2461 aacgcaccag aagaacacat ggtgccaaag gtaccggttg ttacaacatc aactttttta
     2521 aatgctgatt tatatccttc agatttaact atacctgcca tttcttcagc agtgacaaca
     2581 actgcatcac ctttttttat ctttgcgttt atttcttcat agcttttctt tatgacaata
     2641 tcactcatgg tggatttcct ttgtgttata atgtgtggga taccattatg gagccagagc
     2701 ttgaaaactg gcaagtaaaa atttatgatt gaaaggatga ggagcttgca tgtttaataa
     2761 aaattaaaaa aggcttgaaa aaaagaaaat gtaataaaaa ttttatattt aacgacatag
     2821 aaattgcaga tatatgaaaa tttgtgtcta tgtacaaatg gtgaaactta aaaagatgaa
     2881 ttcaattatg atatattgta aaagatcttt gtgatggcag aaaaagaagc aaaagcaaga
     2941 ataaaaatac tgtgttttat aaaaaaatct atataaatgt tacaaggaaa tgaacatgaa
     3001 caacgaaaag attacgctaa gacaacttga aacccatcta tttaaagcag ccgatatttt
     3061 acgaggcaaa atggatgctt cggaatataa agaat
//
LOCUS       DAORYL010000169         3086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_21088_length_3086_cov_8.412075, whole genome shotgun sequence.
ACCESSION   DAORYL010000169 DAORYL010000000
VERSION     DAORYL010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21088_length_3086_cov_8.412075"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PLV81_15280"
     CDS             <1..233
                     /locus_tag="PLV81_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11502.1"
                     /translation="ALHESIPATQDEQLFIDNVWVLAVLDSNGNGKPDNGERIAFYWG
                     YLLFYYPIKLPSPLGDGTTILNKTVRFSSYTY"
     gene            271..2940
                     /locus_tag="PLV81_15285"
     CDS             271..2940
                     /locus_tag="PLV81_15285"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOM11503.1"
                     /translation="MKKWFNIVLTYPKTVIVIIIVITVLLGSGVFKIQFDSSLDAVMP
                     RHDPEYLLNEEVKKTYGNTGKFIIIDVASSDMLQPHILTLVSNLHDDIDEYRRFDEGR
                     EMHRLSLLQELSHNKNITKQMLLQTFADDPVFTRYIMQTLQQLDIEGDRLSQRDMTKI
                     IKKFTRTIEVKRNETVDLIISPFTMKDLRGQNDTLYVRDIVEKDETGKRKVPQTAEEI
                     AEFKKRLTNNPAFEKGIYVKDAQGTITDFGILLRLVDQPQYDPLVKEILDIISYYQGA
                     ADIVIQGVPVTHHEINEYMKRDLERFIPLVLLAVLIVFYLNFRSVRGMLLPFATLVFA
                     DLWVIGLMGHLGFKLNVIGVALPPLMTAVGSSYSIHILNRYYIDYPAISRLGKYKGIM
                     SSSMHISLTVILAALTTALGFFTLITNQVSSIREMGVFAAIGVIFSVIIALSMIPAVL
                     MLLPHKEKKLQFSSKVFAYLSNLSTVDTIVTTLAHWAVKKTPVVMSILFILICIAFFG
                     LSKVNVESSVHAYFKEGDYILVSSKIIGQKFGGSTGLNILIETNKHDGIKNPQFLQFV
                     DTFRDWLVADENIDLNIGRTDAFTDFIKTINLAMHDNDFAYYCIPDTEKDIDSYIDLY
                     GGKDDNDNGLADEFEPYVDRKFTTVNIFARIWEKEGKLLTSSIMHHTINKIGTYLDTH
                     LPDGYTYKITGEPKVLVRMSEYIVRGQIMSLALSLISIFVVVFMLFNNIRAALVALIP
                     ISTAVLLNFGVMGFLGIRLDIATAIIASITIGIGIDDTIHFLNTYRHFDKMNIPQDEV
                     ILKTLHQSGKAIVYTSIALIFGFLVLVISSFKPVIYFAVLLAVTMITTTIGALLFLPA
                     TIYFFKLNLSPKISTSKVGQILNIGKYFELESD"
BASE COUNT          982 a          563 c          573 g          968 t
ORIGIN      
        1 ccgcattaca tgaatcaata ccggcaacgc aagatgaaca gcttttcatt gataatgtat
       61 gggtactggc tgtactggat agtaacggta acggtaagcc agataatgga gaacgcatag
      121 cgttttactg gggttattta ctattttact accctataaa gcttccatca cccttaggag
      181 atggcacaac aatcttaaac aagacagtgc gtttttcaag ttatacatat taagttgcac
      241 aatcaataat aacaataaag gaatcggctt atgaaaaagt ggtttaacat agtgcttacc
      301 taccccaaaa cagtgatagt aattatcatc gtcatcaccg tactattagg cagtggcgta
      361 tttaagattc agtttgatag ctctcttgat gctgtcatgc ccagacatga tcctgaatat
      421 ttactgaatg aagaagtaaa aaagacctat ggcaataccg gcaaatttat catcatagat
      481 gttgcatcaa gtgacatgct gcaaccacac attttaacac tggtatccaa tcttcacgat
      541 gatatagatg aataccggcg atttgatgaa ggcagggaaa tgcaccggct ttctcttcta
      601 caggaactat cgcacaacaa aaacattact aaacaaatgc tgttacagac atttgcagat
      661 gatcctgttt ttacacgcta cattatgcaa accttacaac agcttgacat tgaaggtgac
      721 aggctttcac aacgcgatat gacaaagatt attaaaaaat ttaccagaac cattgaagtc
      781 aaacgcaatg aaaccgttga tttaatcatt tcaccgttta ccatgaaaga ccttcgcggc
      841 caaaacgata ccctgtatgt acgcgatata gtggaaaaag atgaaacagg caaacgaaaa
      901 gtcccgcaaa ctgctgaaga aattgctgaa tttaaaaagc ggcttaccaa taaccctgca
      961 tttgaaaaag gcatttacgt taaggatgct caaggaacaa tcactgattt tgggatactg
     1021 cttcgtcttg ttgaccagcc acaatatgat cctttagtaa aagagattct ggatatcatc
     1081 agttactatc agggtgctgc tgacatagtt atacagggtg tccccgtaac ccatcatgaa
     1141 attaatgaat atatgaaaag agatcttgaa cgctttatac cactggtact tttagcagtg
     1201 ctgattgtat tttatttaaa ttttagatca gtacggggaa tgcttcttcc atttgccaca
     1261 ctggtatttg ctgatctgtg ggttattgga ctcatggggc atctgggatt caagcttaat
     1321 gtgataggcg ttgcattgcc accacttatg acagctgttg gcagttcata ttctattcac
     1381 atccttaacc ggtattatat tgattacccg gcaatttccc gtttagggaa atataaagga
     1441 ataatgtctt catcaatgca tatctcactt acggttatcc ttgctgcttt aactactgct
     1501 ttaggttttt ttaccctcat aaccaatcag gtttcatcca tcagagagat gggagtattt
     1561 gcagcaatag gagttatttt ttctgttatt attgcactct cgatgattcc tgcagtattg
     1621 atgctgcttc ctcataaaga gaaaaagctg caattttcat caaaggtttt tgcatacctt
     1681 agtaatttat caacagttga tacaattgtt actacccttg cacactgggc tgtgaaaaag
     1741 actcccgttg tcatgagcat tttatttatt ctgatatgta ttgcattttt tggcttaagt
     1801 aaagtgaatg ttgaatccag tgtccatgcg tactttaagg agggcgatta cattcttgta
     1861 agctctaaaa taattggtca aaagtttggc ggttccacgg gactcaacat actcattgag
     1921 accaacaaac atgatggaat aaaaaatcct caatttttac aattcgtcga tactttccgg
     1981 gattggcttg ttgctgatga aaatatagat ttaaatattg gaaggactga tgcatttacc
     2041 gatttcatta aaacaataaa tttagccatg catgataatg actttgccta ttactgcatc
     2101 cctgatacag aaaaagatat cgatagctac attgaccttt atggaggaaa agatgacaac
     2161 gataatgggt tggctgatga atttgaaccc tacgttgaca gaaaatttac aacagtaaac
     2221 atatttgctc gaatatggga aaaagaagga aaattattaa cctcaagtat aatgcatcat
     2281 accataaaca agattggcac ctatctggac actcatcttc ccgatggata tacatataaa
     2341 ataacaggcg aaccaaaagt actggtgcgt atgtcagaat atattgtgcg tggacagatt
     2401 atgagcttag ctctcagttt gatttctata ttcgttgtag tattcatgct cttcaataat
     2461 atacgggcag cactagttgc acttattcca ataagtactg cagtactttt aaattttggt
     2521 gttatgggat ttttaggcat acggcttgac attgcaacag caatcattgc atccataacc
     2581 attggaattg gtattgacga taccattcac tttttaaaca cgtatcggca ttttgacaaa
     2641 atgaatatcc cacaggatga agtaattctt aaaaccctgc atcagtcagg taaagcaata
     2701 gtatatacat ccatagcact tatatttggt tttcttgttc ttgtaatatc aagctttaag
     2761 ccagttatat attttgctgt gttgctggct gttactatga taacgacaac cattggtgca
     2821 cttttatttt tacctgcaac catttatttc tttaaattaa atctatcacc aaaaatatct
     2881 acatcaaagg tggggcaaat tctcaatatt ggtaaatact ttgaattgga atcagattga
     2941 tgcattcaaa ctccccaaag gggggaaaaa tatattaatt ataaattcca ttttaatagg
     3001 agggatacaa tgaaaaggtt tctggtattt agtttgttac ctgtgttttg tgtggcgata
     3061 gttattgtgt atgcaatgac aggcag
//
LOCUS       DAORYL010000170         3084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_21107_length_3084_cov_8.241004, whole genome shotgun sequence.
ACCESSION   DAORYL010000170 DAORYL010000000
VERSION     DAORYL010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21107_length_3084_cov_8.241004"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..>3084
                     /locus_tag="PLV81_15290"
     CDS             99..>3084
                     /locus_tag="PLV81_15290"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM11504.1"
                     /translation="MKTKRTMKILFAITCALIIAAITYAQFADNAQSDDKIHTSKITY
                     KNYIWIEGEHAVSTNFAREPIYNFFCSNKFALQLSKDVDPPQDGYFATYVFYVTHTKE
                     YDLWMGCTPPGSAYKDRPGYASPIEWKIDNGPFQLASAENVYVKSFYGIGGFYWVKIA
                     SGTLNAGKHTLTIRVKQKRSSGWDYYFYVDAILLLPQYSTAITQLMDFPEVAPKDFAT
                     PGDGILFNAPEFYEKRLRANISDREALFTLVQIYGWLYDYNKAIFLLQKYLEKNPRDI
                     DMRLQLAAFYSWSDQLDAAISEYKNIISIDEKNITARKLLSVLAGWNNRYDEAIKYYQ
                     EIIAIDPENVDAYISLGTQYSWKGEMNRAIATFEKAETIAPTNIEVLEVLGDNYYWAG
                     RAYDAIRQYYRIIDINEKQISAYKKLAKVYLDLGESAKAGKILNDARQVVDLYPELSG
                     ISLDVKDELAKERLDLIESYKEALVKNPDDINLRKGLVDTYIWNRMYADAVKEYDALL
                     AYKMFHAIEQAEQKLSRLAIHTVGLQSLMPLIQTIKNDVSSIQKKYTKLIDDKKKGKA
                     VNEPLSLASDKALLEGHIAKLKEIQKELYVYNDITAAFGKDVQTYTAMQAATDWHFEP
                     DRIYSYAATAKKLNPQMYQPYKVQGTIDLLYGKPSNAVSFFESVKKLATSAEFPGLPL
                     AYAQTKKYSDAIALLEESLQSKKISTAYKNDIEQILSLTKLNKGEVSSQGSLQDIENL
                     LVNTNSLLDSIGEDVDQKLSFGLATMRSVYEKQFLDLELDNARIYKEIANFYLQNGKF
                     NASREYYSNILKVQPLNVEANFAMGNINELLGYWKDAKENYEICINAQPDFEYARKAH
                     YELQKQHSPSVTGNGIYFSDNIRTRYGIGISGSYEPSQYYTLQAGYEFTKQTDSGGIA
                     TTGQLFYPSDGNVTMHKAFAEIAVPFRFVYMRLFAHLGGTMYSGTVDYGNPLYDTSLS
                     YVSLSYGAGFWWMPPGTGITVKV"
BASE COUNT         1028 a          516 c          674 g          866 t
ORIGIN      
        1 gagccaggat gcatatcctg atgaatcagt aacaggattg caggcaataa tgataattca
       61 aaaattagta aagtaacatt tggggatgag gggtatatat gaaaacaaaa agaaccatga
      121 aaatattgtt tgcaataaca tgtgctctca ttatagcagc aataacttat gcacaatttg
      181 cagataatgc tcaaagcgat gataaaatcc acacttctaa aataacatat aaaaactata
      241 tttggataga aggggagcat gcggtatcaa ctaattttgc tcgtgaacct atttataact
      301 ttttctgcag caataagttt gcgctgcaac tttcaaagga tgttgatccg ccccaggatg
      361 gctattttgc tacgtatgtg ttttacgtaa cgcataccaa ggaatatgat ctgtggatgg
      421 ggtgtactcc acccggatca gcatataagg acaggccagg ctatgcatca cccattgaat
      481 ggaagattga taatggccca tttcagttag cttcagctga gaatgtgtat gtgaaaagtt
      541 tttatggcat tggtggattc tactgggtaa aaattgccag tggtacactc aatgccggaa
      601 aacacaccct gacaataaga gtaaaacaaa aaagaagcag tgggtgggat tattactttt
      661 atgttgatgc cattttacta ttaccacagt acagcactgc tataacacag ctcatggatt
      721 ttccagaggt tgcaccaaaa gattttgcca ctccgggaga tggtatattg ttcaatgccc
      781 cagagtttta tgaaaaaaga ttgcgtgcaa atatcagtga cagggaagca ctgtttactc
      841 ttgttcagat atatggatgg ctgtatgatt ataataaggc aatatttctg ttgcagaaat
      901 atttagaaaa aaatccccgt gatattgata tgcgattaca actggctgca ttttattcat
      961 ggtcggatca gttggatgca gctatcagtg aatataaaaa tatcatatca attgatgaaa
     1021 agaatataac agcacgaaag cttctttcag tgctggcagg atggaacaac cgctatgatg
     1081 aagcaataaa gtattatcag gaaattatcg caatagatcc tgaaaatgtg gatgcgtata
     1141 tttcgctggg aactcagtat tcgtggaaag gtgaaatgaa cagagctatc gccacatttg
     1201 aaaaggctga aactattgca cccactaata ttgaagtgct tgaggtttta ggagataatt
     1261 attactgggc aggaagggca tatgatgcca tacggcagta ttaccgcatc atcgacataa
     1321 atgaaaagca gatatcagca tataagaaat tagccaaggt gtatctggat ctgggtgaaa
     1381 gtgcaaaggc tggtaaaata cttaatgatg caagacaggt tgtggattta tatccggagc
     1441 tatcgggcat atcgcttgat gttaaagatg agcttgcaaa agagcggttg gatttaatag
     1501 aaagctataa agaggcactg gtaaaaaatc ctgatgatat caatttacga aaagggcttg
     1561 ttgatacgta catatggaac aggatgtatg ctgacgcagt taaagaatac gatgccctgc
     1621 ttgcatataa gatgtttcac gcaattgagc aggcagagca aaagttatcg cggttagcca
     1681 tacacactgt tgggttgcaa tctttgatgc cattgataca aaccattaaa aatgatgtca
     1741 gttcaattca gaagaagtat acaaaactca tagatgataa aaagaaaggc aaagctgtca
     1801 atgaaccatt gtcccttgcc agcgataaag ccttgcttga agggcatatt gcaaaattaa
     1861 aagaaatcca gaaagagctg tatgtatata atgatattac tgctgcattt ggcaaagatg
     1921 tgcaaacgta tacagcaatg caggctgcaa cagactggca ttttgaacct gataggattt
     1981 acagttatgc tgcaacggca aagaaattaa atccacagat gtatcagcca tacaaggttc
     2041 agggaaccat tgatttgttg tatggaaagc catcaaatgc agttagtttt tttgagtcgg
     2101 taaaaaaatt ggctaccagc gctgaatttc ctggattgcc gcttgcatat gcacaaacaa
     2161 aaaaatacag cgatgcgata gctcttttag aagagtcatt gcagagtaaa aaaatttcca
     2221 cagcatataa aaatgatatt gaacagatac tatcgctaac aaaattaaat aagggggaag
     2281 tttcttcaca gggttcactt caggatattg aaaatttact ggtaaatact aactctttac
     2341 ttgactctat tggtgaagat gttgaccaga agctttcatt tggtcttgca acaatgcgct
     2401 cagtgtatga aaagcagttc ttagacctgg agcttgacaa tgcacgtatt tataaagaga
     2461 tagccaactt ttatctgcag aacggcaaat ttaatgcatc acgagaatat tattcaaata
     2521 ttctgaaagt gcagccattg aatgtggaag ctaattttgc tatggggaat attaatgagc
     2581 ttctgggata ctggaaggat gctaaagaaa actatgaaat atgcattaat gctcagcctg
     2641 attttgaata tgccagaaaa gctcattatg aattacagaa acagcattcc ccttctgtta
     2701 caggtaacgg catatatttc agtgataata tacgaacgcg gtatggcatt ggtatcagtg
     2761 gttcgtatga gcccagtcag tattatacat tacaggcagg ctatgaattt acaaaacaga
     2821 ctgattcagg aggaatagca acaacggggc agcttttcta cccttctgat ggcaatgtga
     2881 caatgcataa agcatttgct gaaattgctg ttcccttcag gttcgtatat atgcggcttt
     2941 ttgcacatct gggaggtacc atgtattcgg gtacggtaga ctacggcaat cctttatatg
     3001 atacatcact gagttatgtt tccttatcat atggggctgg gttctggtgg atgcctcctg
     3061 gaacaggtat caccgtaaaa gtgg
//
LOCUS       DAORYL010000171         3013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_21746_length_3013_cov_13.206558, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000171 DAORYL010000000
VERSION     DAORYL010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21746_length_3013_cov_13.206558"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..1971
                     /locus_tag="PLV81_15295"
     CDS             82..1971
                     /locus_tag="PLV81_15295"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOM11505.1"
                     /translation="MNIVFIQLPLLDHGYNYIAGNIPNAPATLQGFCLSNYRTNIKTI
                     QLPHTIQNFGSDPMIVDTILQYNPTHVAFTCYLWNVERSRSIAQKIKALTPQVTILFG
                     GPEIQLQAHILQHYHPEIDIFIIGEGEFFFEHYVHNTHTQYLHTINGNTVFIQPAHHF
                     TPLKKMVEPYTQGYLQPMYDGSLSVEVIRGCPFTCNYCLYSKNTSRVRALPAQTLSAI
                     VHKAKKENIQEIYLLAPTFNQSKHFAHYCNALIQAENTIPLHTEIRADRITQESIDLL
                     KKAGFKSLEVGLQTMNQPCLDHVGRKTNAQKELEGIVRLKDAGFKLQVGIIPGLPTDT
                     PESFMNTIDTLLDYGLGNEIELYPLMVLPGTALYDEALQHSATFMQHPPYYFIEGFNF
                     SQTDLHTITQYFEDATGFVFNPPFIPSFVMNKSGTLTNSVLINCKKHTTIPGHIFNAL
                     QTNHIIFYLLCDSSLHIDLALQSIISQCQFHTMLYSVVCMYNDILDDNAIANTLLAAT
                     QDTFYYHMMHFQDTYDRLPVRIFQLFETLETFLRAQEHYACINPILLVTPENYTRISQ
                     KDFAYPFPIVIQKGFARHCINWLKQQYSESIELVSFQDEADQKYVYNELGIEYVKIVF
                     NTYFP"
     gene            2589..>3013
                     /locus_tag="PLV81_15300"
     CDS             2589..>3013
                     /locus_tag="PLV81_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11506.1"
                     /translation="MNIETTTCIAYENVKLLQDYADVYKLSLHTFIINFITYVMSYKK
                     IPVKSCRRLTYRKRYERWKRVHLYLYENEYEFLMDVRKVCKMSIAKVIAYCIENYLFD
                     FLAALDSEDNTDNYRFSEYTFMFYLENGIQCCRFYWGPHP"
BASE COUNT          940 a          631 c          511 g          931 t
ORIGIN      
        1 caattcatgc tctgacccca ctattttgga tttcctataa aattattgct atgtttgcag
       61 tatcatcgct agtatattac catgaacatc gtattcatac aactcccatt gctagatcac
      121 ggctacaact acattgctgg caatatcccc aatgctcctg ccacactgca aggtttttgc
      181 ctcagtaact atcgcacaaa tattaaaacc atacaattac cgcacacaat acaaaacttt
      241 ggctctgacc ccatgattgt tgataccata cttcaataca atccaaccca tgtagctttt
      301 acctgttatc tgtggaatgt tgaacgctca cgctctatag ctcaaaaaat taaggctctg
      361 accccacagg taaccatact gtttggaggt cccgaaatac agctacaggc tcatatactg
      421 caacactacc atcctgaaat agatatattc atcattggcg agggtgaatt tttctttgag
      481 cactatgtgc acaatacaca tacgcaatac cttcatacca ttaatggcaa taccgtattc
      541 atacaacctg cccaccattt tacaccactg aaaaagatgg ttgaaccata tacacaggga
      601 tacctccaac ccatgtatga tggaagcctc agtgtggagg taattcgcgg gtgcccgttt
      661 acatgcaact actgcctgta ttcaaaaaat accagcaggg tcagagcatt gcctgctcaa
      721 actctctcag caattgtcca caaggcaaaa aaagagaata tccaggaaat atacctgctt
      781 gcaccaacgt ttaatcagtc aaaacatttt gcacattact gtaacgccct catccaggct
      841 gaaaacacca taccgctgca tactgaaatc agagctgatc gcatcacaca agaatcaata
      901 gatctattaa aaaaagcagg tttcaaaagc ctggaggttg gcctgcaaac aatgaatcaa
      961 ccatgtcttg atcatgtagg ccgaaaaacc aatgcacaaa aagaacttga aggcattgtg
     1021 cgccttaaag atgctggctt caaacttcag gttgggatta ttccagggtt gcccaccgac
     1081 acgcctgaat cgtttatgaa caccattgac acccttcttg attacgggct tggcaacgaa
     1141 attgaactgt atccgcttat ggttttgcca ggaactgccc tgtatgatga agcactgcaa
     1201 cacagcgcca cctttatgca gcacccaccg tactacttca ttgaggggtt caatttttca
     1261 caaactgatt tacacacaat aacacagtat tttgaagatg caacgggatt tgtgtttaat
     1321 ccaccattca taccttcatt tgttatgaat aaaagcggca cgcttactaa cagtgtgctc
     1381 atcaattgta aaaaacacac aacaatacca ggacatattt tcaatgccct gcaaacaaat
     1441 catattattt tttatttgtt gtgcgatagt tcattgcata tagaccttgc cctgcaatct
     1501 atcatttctc agtgtcaatt ccataccatg ctgtattccg ttgtttgtat gtacaacgat
     1561 atactggatg acaacgctat tgccaacaca ctgttagctg ccacacagga caccttttat
     1621 tatcacatga tgcacttcca ggacacgtat gacagattac cagtgcggat atttcagtta
     1681 tttgaaacgc tggaaacatt tttacgcgcg caggaacact atgcgtgtat taatcccata
     1741 ctgcttgtaa cacctgaaaa ttatacaaga atttcacaaa aagattttgc atatcccttc
     1801 cccattgtta tacagaaagg ttttgcacgc cactgcataa actggcttaa gcaacagtac
     1861 agtgaaagca tagagctggt aagctttcag gatgaagccg accagaaata tgtatataat
     1921 gaacttggta tagaatatgt aaaaattgtt tttaatacct actttccata aattcttaac
     1981 tttcattaat aattataatt tcaatataaa ttggtaatat ttcacaaaac ttaatcgtga
     2041 ttgtttgtaa gcgtcaaatt aaccataggg gtattgatat gcgatgaaat tttaaaaagc
     2101 aaggaaagtt tatttttttt attctattgt ttcagataag caatatagta ttaaaccata
     2161 acctgtgtca ccactttacg gtaaagtcag caatgaaata taattgtaat ttacgaaacc
     2221 accatgtaaa ttgattatta tcaaaattat gttccctcca ctttgctgct ttattttggc
     2281 cccttattcc atactgccgt atgtgctctt cacactactg ccgctttgtg tttctggtaa
     2341 atgtgcttgt ttcattactg tttgccacct tatggacaat gttttatttt tatgagggtg
     2401 gtagttatgt gagtgggtaa tttatatggt ttctaaaatt atatgtatat acaaaaacat
     2461 atttcatcgt tccgtgtatc tattaatcat gtattgctac attttcattt ctacgcttaa
     2521 attatgattg caaattgtct tagcatatca tagtaacgat gatacacata tacttctgga
     2581 gggaaatcat gaatatagaa acaaccacct gcattgcgta tgaaaatgta aaacttttgc
     2641 aggattatgc tgatgtctac aaactatcgc ttcatacgtt tattattaat tttattacct
     2701 atgtgatgtc atacaaaaag attccggtaa aaagctgcag gcggcttacg tatcgcaagc
     2761 gctatgaacg ctggaagcgg gtgcatctgt atctgtacga gaatgagtat gagtttttaa
     2821 tggatgtgag gaaagtgtgc aagatgtcca ttgctaaggt tattgcctac tgtattgaaa
     2881 actatctttt tgattttctt gcagcgcttg atagtgaaga taataccgat aactatcgat
     2941 ttagcgaata cacatttatg ttttatttag aaaatggcat acaatgttgt cgattttact
     3001 ggggaccaca ccc
//
LOCUS       DAORYL010000172         2980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_22039_length_2980_cov_12.425299, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000172 DAORYL010000000
VERSION     DAORYL010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22039_length_2980_cov_12.425299"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..813)
                     /locus_tag="PLV81_15305"
     CDS             complement(49..813)
                     /locus_tag="PLV81_15305"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HOM11507.1"
                     /translation="MSTSIIYLFAFLFGASWGSFLYTFIIRYTNGMYIKNPIHALTYP
                     SHCLYCKQKIKPVYLIPVFGYFLTKGRCSVCGKPISFLYVSAEIACGYIAITSIYFFN
                     VAIASLYFIIITTSICIAIIDFLTMEIPDFLVGIIFLTGIVICLFERQWFEHIQGLVL
                     MVAVFIVPLILVKGGFGGGDVKFAGAIGFTLGLHETIVALEIALVTGALYGIAYAVFK
                     SKTLKAHIPFGPFLTLGFITALLFGKDIIQIYFSFF"
     gene            complement(817..1497)
                     /locus_tag="PLV81_15310"
     CDS             complement(817..1497)
                     /locus_tag="PLV81_15310"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOM11508.1"
                     /translation="MKKTYHITSIACIILLCLVLMVQGAYAIGEFSLGLNAGITYDPN
                     HLEPAITQWNTQAKTTATSVEQITIPYSFAWGINMRYQFNYFLFRIGAHFTSPYSVRG
                     KADANKVTISTYQFGLPATVGLIMPLKQRTYFFIGAGITYNYAYLEWKETVPANKYRF
                     ADSIPGFHYMMGAEAPITSKITLSVEWMHQEGRSIPIKDEIAGTTKQTISVKGDFLLF
                     GVNYYIPM"
     gene            complement(1509..2258)
                     /locus_tag="PLV81_15315"
     CDS             complement(1509..2258)
                     /locus_tag="PLV81_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11509.1"
                     /translation="MYYKLCIVLLCCAIALVPVSSLASPFNQAISLYAGYIPSLGGNL
                     YSYEQENTFGSKTGIDGINKSQTGFSTATINRLLGATTGATFKLVMYDFFTARIGANY
                     FKSVWGGKGKTVYNDGADRLLECNYSFDGFDIPVTVGIIIPFYKDMKITFSCGIAYAW
                     GRYKNKFESDAPLSYKGQFKGSAYPLVINTEGEYFINDKFAISTSLSYYRGTTNVIKD
                     SKKGDGSTDYAPIDFSGYRFLLGVSYYFKPI"
     gene            complement(2248..2904)
                     /locus_tag="PLV81_15320"
     CDS             complement(2248..2904)
                     /locus_tag="PLV81_15320"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOM11510.1"
                     /translation="MFKKAIIYISILIFIVGANTSAFAQRYFGVGIHVGGQHDVGDIT
                     SFYPAAQTEPQNSAIFGVAIRLSIPFFFIRTGGDASFTINKGKVLEDQATVGVDEYNV
                     QYTMLPLYAGLQFPLRDRGAFYVGGGVSYFYGTGSVKLSNGTSQDIDATAFGAGFLAG
                     MQLHVTSSFSLYMEWIYVDARSKPVISTTGTYNANNYKDLFIDYSGHRIMIGVMYYVL
                     "
BASE COUNT          987 a          565 c          584 g          844 t
ORIGIN      
        1 agaaatttgg gtaaggcaag gaaataaaga gggtcagagc agtatatttt aaaaaaaaga
       61 aaaatatatc tgaataatat cttttccaaa caataatgct gtaataaatc ctaatgttaa
      121 aaatggacca aacggtatgt gtgctttaag cgttttgctt ttaaacactg cataagcaat
      181 tccatacagt gcaccagtga ccagagctat ctccagtgct actatcgttt catgtagtcc
      241 aagagtaaat cctattgcac cagcaaattt tacatcacca ccaccaaaac cacctttgac
      301 taaaataaga ggcacaataa aaaccgcaac catgagtact aatccctgta tgtgttcaaa
      361 ccattgtctt tcaaataaac aaatcacgat gccagttaaa aaaattattc ccaccaaaaa
      421 atcaggaatt tccatggtta gaaaatcaat tatggcaatg catatgcttg tagtaatgat
      481 gatgaaatat aaagaagcta tcgctacatt aaaaaaatat attgaggtta tagctatgta
      541 tccacaggcg atttcagctg acacgtataa aaaagaaatg ggcttcccac atacactgca
      601 gcggcctttt gtaaggaaat atccaaacac gggtataaga tatacaggtt taatcttttg
      661 cttacaataa aggcagtgcg atggataggt gagggcatga attggatttt tgatatacat
      721 accattggta tagcgaatga tgaaggtata taaaaagctt ccccagctgg caccaaaaag
      781 aaaggcaaaa agatagataa tagatgtact catagattac ataggtatgt agtagttgac
      841 accaaaaaga agaaaatcac ctttgactga tatggtttgt ttggtggttc ctgcaatttc
      901 atctttaatg ggaatggagc gtccttcctg atgcatccat tccactgaaa gtgtaatttt
      961 ggaggttatg ggtgcttcag ctcccatcat gtaatggaat ccagggatgg agtcagcaaa
     1021 gcgatattta ttagcaggta cagtttcttt ccattccagg tatgcatagt tatacgttat
     1081 gcctgcacca ataaaaaagt atgtacgctg ttttagcggc atgattaggc caactgtagc
     1141 gggcaggcca aactgatatg ttgaaatggt taccttgttt gcatcagctt taccgcggac
     1201 actgtaaggg ctggtgaaat gtgcgccaat tctgaataga aaataattga actgatagcg
     1261 catgttgatg ccccaggcaa atgaataggg gatggttatc tgctcaactg aggtagcagt
     1321 tgttttggcc tgagtattcc attgagttat ggctggttcc agatggttag gatcataggt
     1381 aatgcctgca ttaagcccta atgaaaattc gccaattgca taggcacctt gtaccattaa
     1441 aactaaacat aatagtatga tgcatgctat cgatgtaatg tgatatgttt ttttcattct
     1501 gatcatggct atattggttt aaaataatat gatacaccta aaagaaaacg gtatcctgaa
     1561 aagtcaatgg gtgcataatc agtgctgcca tctccctttt tagagtcttt aatgacattt
     1621 gttgtaccac ggtaatatga tagagaagtg gatatggcaa acttatcgtt gataaaatat
     1681 tcgccttccg tgttgatgac cagtggataa gcacttcctt taaattggcc tttgtatgac
     1741 aggggggcat ctgactcaaa tttattctta taccgtcccc aggcataggc tatgccgcat
     1801 gaaaacgtta ttttcatatc tttataaaat ggtataatga tgccaacagt aacaggtata
     1861 tcaaagccat caaagctgta attgcattca agaagcctgt cagcgccatc attataaact
     1921 gtcttgcctt tgccacccca aacgctttta aagtaatttg caccaatacg tgcggtgaag
     1981 aaatcataca tgactaactt aaaggttgca ccagttgttg cacctaacaa tcggtttatg
     2041 gttgcagtag aaaatccagt ctgcgattta tttatgccat caattcctgt tttcgagcca
     2101 aacgtatttt cctgctcata cgagtaaaga ttgcctccta acgatggaat ataccctgca
     2161 taaaggctta ttgcctggtt aaatggcgaa gccagggaag atactggtac aagagctatc
     2221 gcacaacaca ataatactat acatagttta taatacatag tacatgacac ctatcatgat
     2281 tctgtggcca ctataatcaa taaaaaggtc tttataatta ttggcattat atgtcccggt
     2341 ggttgaaatg actggctttg aacgggcatc aacgtatatc cattccatat acagtgaaaa
     2401 agagctggtt acatgcagtt gcatgccagc taaaaagcct gcaccaaagg ctgtagcgtc
     2461 aatatcctgg gatgtaccat ttgaaagctt caccgaacct gttccataaa agtatgatac
     2521 tccaccacct acataaaatg cccctctgtc acgcaggggg aattgcagtc cagcataaag
     2581 gggaagcatg gtgtattgta cattatactc atcaacgccc acagtagcct ggtcttctaa
     2641 aaccttgcct ttattaatag taaaggacgc atcgccacct gtgcgaataa aaaagaaagg
     2701 aattgataat cgtatagcca cgccaaagat ggcactattt tgtggttcag tttgtgcagc
     2761 aggataaaag gacgtgatat caccaacatc gtgctgcccg ccaacgtgaa tgccaactcc
     2821 aaagtaacgc tgggcaaatg cagaagtgtt tgcgccaaca ataaaaatca gtattgaaat
     2881 ataaataata gcttttttaa acatccataa accttctgta tggtactaac atcgacaata
     2941 aatactataa tcaaaagaaa aaatgtcaag acagattttt
//
LOCUS       DAORYL010000173         2935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_22474_length_2935_cov_4.536111, whole genome shotgun sequence.
ACCESSION   DAORYL010000173 DAORYL010000000
VERSION     DAORYL010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22474_length_2935_cov_4.536111"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PLV81_15325"
     CDS             complement(<1..266)
                     /locus_tag="PLV81_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HOM11511.1"
                     /translation="MKWLLVLGAESDIAKAVVRRFAKEGINFYLAHFNIKQIEDFAKD
                     VSIRFGVKTIPVTFDAVKYNTHKAFYDKLSPKPDGALCVFGYLG"
     gene            complement(263..1588)
                     /locus_tag="PLV81_15330"
     CDS             complement(263..1588)
                     /locus_tag="PLV81_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOM11512.1"
                     /translation="MKLTAWGNYPIVDAQVDYLESVESLKNLILSKQKLIAYGNGRSY
                     GDQALNERVIFTKKYNYITDFDSQKGMITCQCGVTLEEILDVIVPKGWFLQVTPGTKY
                     ITVGGAIASDVHGKNHHKEGTFCDHVMSFELMLPDGKVITCSRKNNPEIFKATCGGNG
                     LTGVILKATFRLKKIESAYIKQRTIRAQNIEELIKAIEDNEHYTYAVAWIDCVTKGKN
                     LGRGVLLLGEHATVDDLPLKKRKNPFVLPQKKKLTVPIFFPNFTLNKFSIKAFNWLYY
                     HKTPKGVSESIIDYNTFFYPLDAIDNWNRIYGRRGFTQYQFVIPKEAGHEAMQVIMEK
                     ITGSGMGSFLAVLKTAGKHNGNYLSFPMEGYTLALDFAINTKLFSFLNELDELVMKYG
                     GRLYMTKDVRMGSRFFKSSYPDIKKFIEVRKKIKADFLFESLQSKRLGI"
     gene            complement(1619..2011)
                     /locus_tag="PLV81_15335"
     CDS             complement(1619..2011)
                     /locus_tag="PLV81_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOM11513.1"
                     /translation="MNLAIKYIIFAGVATAVNIFSQWLSFIIYNGFFSLYIAMFVGTI
                     AGLLVKYALDKRYIFYYETKRKSDDIKKFFIYSCMGVITTFIFWGTEICFATIWHHEI
                     SKYIGAVIGLSIGYITKYNLDKRFVFIS"
     gene            complement(2008..2805)
                     /locus_tag="PLV81_15340"
     CDS             complement(2008..2805)
                     /locus_tag="PLV81_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HOM11514.1"
                     /translation="MFFAFMITLHNVLLVVAGIAIFSLCASAVYVFNDIYDVAEDRQH
                     PDKRKRPIASGQISIKFAWLLAFVLFSVSITMAYFFNINFFIILIVYVAINIFYTVVL
                     KHIAIVDITIIAIGFVLRLFAGASIINVQTSMWIVLVTFWLALFLALAKRRDEYLHYI
                     NGKKVRKNIDGYNLEMINAGMVLLATITVVAYIMYSVTPAVMERMGSDKLYLTSIFVV
                     VGILRYMQLTFVYEKSGNPTDVLLTDRFLQMVIALWLLSFYVIHVLA"
BASE COUNT          973 a          570 c          424 g          968 t
ORIGIN      
        1 tcacctaaat aaccaaaaac gcaaagggcc ccatcaggtt ttggcgatag tttatcataa
       61 aaagctttat gtgtgttata tttaaccgca tcaaaagtaa caggaatggt tttaactccg
      121 aaacggatag atacatcttt cgcaaaatct tcaatttgtt taatattaaa atgtgcaagg
      181 taaaaattta ttccttcttt tgcgaaccta cgtactacag cctttgctat atcagattct
      241 gcacccaata caagtaacca tttcatattc ccaacctctt tgattgcaat gattcaaaaa
      301 gaaaatctgc ttttattttt tttcttactt caataaattt ttttatatca ggatatgatg
      361 acttaaagaa tcttgatccc atgcggacat cctttgtcat ataaagtctt ccaccatatt
      421 tcataactag ttcatcaagc tcattgagaa atgaaaataa ttttgtattt attgcaaaat
      481 caagtgccag tgtatagcct tccattggaa atgagagata gttaccattg tgtttgcctg
      541 cagtctttaa tactgctaaa aaagaaccca tgccactacc tgttattttt tccattatta
      601 cttgcattgc ttcatgccct gcttcttttg gtatcacaaa ctgatattgt gtaaaaccgc
      661 gtctgccata tatcctgttc cagttatcaa tagcatccaa tggataaaaa aatgtattgt
      721 aatcaataat tgattccgaa acacctttag gtgttttatg ataatagagc cagttaaatg
      781 ctttaattga aaatttattc aatgtaaaat ttggaaagaa tattggcaca gttaattttt
      841 tcttttgtgg caaaacaaat ggattttttc ttttttttaa gggcaaatca tcaacagttg
      901 catgttcgcc taacagtaaa actcctctac ctaaattttt accttttgta acacaatcaa
      961 tccaggcaac agcataggta taatgctcat tatcttctat tgcctttatg agttcttcaa
     1021 tattttgcgc tcgtattgtt ctttgtttta tatatgctga ttcaatcttt tttagcctga
     1081 aagtagcttt taaaattaca cccgtcaagc cgttcccgcc acaggttgct ttaaagattt
     1141 cagggttatt tttgcgtgaa caggtaatta ctttcccatc aggaagcatg agttcaaatg
     1201 acataacatg atcgcaaaag gtaccttctt tatggtggtt ctttccatgg acatctgaag
     1261 caatggctcc ccccaccgta atgtattttg tcccaggggt aacctgtaaa aaccatccct
     1321 tgggaacaat aacatccaat atttcttcca gcgtcacacc acattggcag gttatcatgc
     1381 ctttttgtga atcaaaatct gtaatatagt tatatttttt cgtaaaaata acgcgctcat
     1441 tcaatgcctg atcgccataa ctacggccat tgccataggc aataagcttt tgttttgaaa
     1501 gaatgaggtt ttttaaggat tcaactgatt ctaaataatc tacttgtgca tcaacaatgg
     1561 ggtagtttcc ccaggcagtg agtttcatgg ttataccttt ttactatata gttttttatc
     1621 acgaaataaa aacaaagcgt ttgtcaaggt tgtattttgt aatgtaacct atgcttaagc
     1681 caataactgc accaatatac ttacttattt catggtgcca tatagtagca aaacaaattt
     1741 ctgttcccca gaatataaaa gtagttatta cacccatgca cgaatatata aaaaatttct
     1801 taatatcatc gctttttcgt tttgtttcat aataaaatat atatcgctta tcaagagcgt
     1861 actttacaag taaacccgca attgttccaa caaacatagc tatatacaac gaaaaaaatc
     1921 cattataaat aataaaactt aaccattgac taaaaatgtt tactgctgtt gcaactcctg
     1981 caaaaataat atatttaatt gccaaattca tgctaataca tgtatcacat aaaatgacaa
     2041 aagccacaat gcaatcacca tctgcaaaaa cctatccgta agcaatacat cggtaggatt
     2101 accacttttt tcatatacaa aggttagctg catataccgc aatatcccaa caacaacaaa
     2161 aatgcttgtt aaatataact tatcgcttcc cattctttcc attactgccg gcgttactga
     2221 atacattata tatgccacaa cagtgatagt tgcaagaagc accattcccg catttatcat
     2281 ttcaaggtta tagccgtcaa tattttttct taccttttta ccatttatat aatgtaaata
     2341 ttcatcacgt cttttggcaa gagctaaaaa gagagccagc cagaaggtaa caagtactat
     2401 ccacattgat gtctgcacat tgattataga agcacctgca aaaaggcgta gcacaaaccc
     2461 tattgctatg attgtgatat caactatagc aatgtgcttt agcaccactg tataaaaaat
     2521 atttatggca acatacacaa ttaaaataat aaaaaagttt atattaaaaa aataggccat
     2581 agtaattgac acggaaaaaa gcacaaaagc aagcaaccat gcaaacttaa ttgatatttg
     2641 accgcttgca ataggccttt ttcttttatc aggatgctgc ctgtcttccg ctacatcata
     2701 gatatcgttg aacacatata ccgcacttgc gcataatgaa aatatagcaa ttcctgccac
     2761 aactaacagc acattatgca gcgtaatcat aaaggcaaaa aacatgggtg ctgctatgaa
     2821 aaaatttttt acccattgat gggggcggag gagtgaaaat aaattttgta ttcttttcat
     2881 ttaactacca atggaacttc caatatattg tttttatctt tgtcaccaaa ccagg
//
LOCUS       DAORYL010000174         2923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_22594_length_2923_cov_8.899930, whole genome shotgun sequence.
ACCESSION   DAORYL010000174 DAORYL010000000
VERSION     DAORYL010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22594_length_2923_cov_8.899930"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            759..1058
                     /locus_tag="PLV81_15345"
     CDS             759..1058
                     /locus_tag="PLV81_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11515.1"
                     /translation="MKKITIIVSIILVVSVVVFAKGKSFTGRLIFVGKDYIEVKYGKT
                     EKVFYIQEKSVFIKNNANATFADLEPCQIVKVAYTAGGNKLNIIRCEIIKQSDCK"
     gene            1072..2904
                     /locus_tag="PLV81_15350"
     CDS             1072..2904
                     /locus_tag="PLV81_15350"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOM11516.1"
                     /translation="MQLTFDDHSFEILYKTHNTAKVASINTLQHDNYLDDNLNVQYTR
                     NRDAIQCIIKPITRLYIQKAILSLQVLTSDDIACFANGYQSWTYTGPFFKDSKKRKPW
                     PQFVIANQENAAHPPKGKRGVVQSDMIFVLGDSNQQNAYVFGHLPVHKQFHQFVSFEY
                     NYRTKLLSIIWDVDRFCDAEEDYTLDTVCILQGTVWKTMEYYAETIAQELSPTFNNTL
                     RKGWCSWYYYYNTITRDEILLNAKYAKENSIPFDFIQIDDGYQATIGEWTTVRGSFTA
                     AMRYIADTIKGYGFTPGIWYSPFIVSKDSRIFKENKSWILKDNNGKPVIAGFNPAWGG
                     YYYTVDVTQPAALSWIEDTINTLLYEWGYEYLKLDFMYAAALPGVRHNTTISRYEALA
                     QAVARIRKASGNAFILGCGMPLQAGIRYVDAMRIGPDVAPQWGRTFFDKLFNSDSGIS
                     TRSAICSSIYRSFMHNRFWVNDPDCLMIRQHKTKLNPEERQTLYNVITALGGMLVISD
                     RLPDYSATEREMLLQAIALFDKAKDGDIYCNDVLRPLRSFYNAKGLGVLLNVDDTTCE
                     TTLEQEIMNNYSKIFLIEKNTKIPSHTKNFDLMPHSSKLFLFEK"
BASE COUNT          976 a          458 c          554 g          935 t
ORIGIN      
        1 tttaaaaatg tggagagctt atactataaa gcttgtggaa gaacataaat atatcaatgt
       61 aaagcaaaat gattattagt ttttgtcaag attaaaacac atcaatttaa aaatatttaa
      121 cacaggctca cagggctatc aaagggggtg tcccaaaatt ggccgttgag tgaataccat
      181 ctacattatt tatcataatc cgctcatttc gatacatttt tgccacaaaa tgtggcaaaa
      241 ctactcaatg agcgtaaaga aatttttatc aaaacaactt ttgggacacc ccctatatat
      301 atttatttga acatttttta cataatagtg attgttacta tacatttctg aatgtaacat
      361 attggagatc ggttacttgt tctgtggtga aaagttgtac agtgtttatg taaagatttg
      421 taaaattaga aagcatttcc gtaaaaaata atgcaacaaa ttaactataa aaagtgggtt
      481 aatagtgtaa ttggtttgcc tttgataaaa aataaaactg gattacacca tatggtattt
      541 atatggtatc aaccacctgt ggttagtttg acgcttactc tttcgctcat tatatcatta
      601 cctctttggt aatgaatttt acagtgttga ttaaaatacg atattttata tttaaatcat
      661 aaaatattgt tgcagaaaat ataattgttt ttattctatt taatgactat aagtagtata
      721 aatggcatat aatgtcgttt acaataggag gttgtactat gaaaaagata accattattg
      781 tatcaattat tctggttgtg tcagttgttg tttttgcaaa aggaaaaagc tttacaggcc
      841 gtttgatatt tgttggtaaa gattacatag aggtgaaata tggaaagaca gagaaagtat
      901 tttatataca ggaaaaatcc gtatttataa aaaataatgc taatgccacc tttgcagatc
      961 ttgaaccatg ccagattgtt aaggttgctt acacagcagg tggtaataag ttgaatataa
     1021 tcagatgcga aataataaaa caaagcgact gcaaataaaa aaggatacct tatgcagcta
     1081 acgtttgatg accatagctt tgaaatactt tataaaacac ataatactgc taaagtagca
     1141 tcaatcaata ctttacagca tgataattat ttagatgaca atctcaacgt tcagtacact
     1201 cgtaacaggg atgcaataca gtgtataatt aaaccaataa cgcgtctgta tatacaaaaa
     1261 gcaattctgt cgctacaggt tttgacttcg gatgatatag catgttttgc gaatggatat
     1321 cagtcatgga catatacagg tccatttttt aaagattcaa aaaagcgcaa accctggccg
     1381 caatttgtca tcgcaaatca agaaaatgca gctcatccgc ctaaaggaaa acgtggtgta
     1441 gtccaatcgg atatgatatt tgtattgggc gatagtaacc agcaaaatgc gtatgttttt
     1501 ggacatcttc ccgtacataa gcaattccat cagtttgttt catttgaata taactatcgc
     1561 acaaaattgt tatccattat atgggatgtt gatagattct gtgatgctga ggaagactac
     1621 acgctggata cagtatgtat tttgcagggt acagtgtgga aaacgatgga atattatgca
     1681 gaaactattg cacaggaact atcgcctaca tttaataaca cactacgcaa gggctggtgt
     1741 tcctggtatt attactataa taccattacc agagatgaaa tacttctcaa tgctaaatat
     1801 gcaaaagaga attccatacc atttgatttt atccagattg atgatgggta tcaggctact
     1861 attggcgagt ggactactgt caggggatct tttaccgctg ccatgcgcta tattgctgat
     1921 actatcaaag gatatggatt tacgcctgga atctggtatt ctccatttat tgtttcaaaa
     1981 gattcaagga tttttaaaga gaataaatcc tggattctta aagataacaa tgggaaacct
     2041 gttattgctg gtttcaatcc tgcatggggt ggctattact ataccgttga cgttactcag
     2101 cctgcagcgc tgagctggat tgaggatact attaatacgt tattgtatga atgggggtat
     2161 gaatacctca agttagattt tatgtatgct gcagcactac cgggagtgcg acacaacacg
     2221 acaatatcac ggtatgaagc tcttgcacag gcggttgccc gcatacggaa agcaagcggg
     2281 aatgcattta tactgggatg tggtatgcct ttgcaggcgg gcattaggta tgtagatgca
     2341 atgcgtattg gtcctgatgt tgcaccgcag tgggggcgca cattttttga taaactattt
     2401 aacagtgatt caggaatttc aacacgttct gctatttgca gcagtattta tcgttcattt
     2461 atgcataacc gcttctgggt taacgaccct gattgtctta tgatacgtca gcataaaaca
     2521 aaacttaatc ctgaagaacg gcaaacgctt tataatgtaa taacagcgct gggtggtatg
     2581 cttgtaatat ccgataggtt acctgattat tctgctactg aacgtgaaat gctattacag
     2641 gcaattgcat tatttgataa ggcaaaggat ggagatattt attgtaatga tgtattgagg
     2701 ccattacgtt cattctataa tgcaaaaggt ttgggtgtat tattaaatgt agatgatact
     2761 acttgcgaaa caacacttga acaggaaata atgaataatt attctaaaat atttttgatt
     2821 gaaaagaata caaaaatacc ctcccacaca aaaaactttg atttaatgcc tcacagttca
     2881 aaattatttc tttttgaaaa ataattttgc aaccgtttac tga
//
LOCUS       DAORYL010000175         2922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_22603_length_2922_cov_12.551796, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000175 DAORYL010000000
VERSION     DAORYL010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22603_length_2922_cov_12.551796"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..478
                     /locus_tag="PLV81_15355"
     CDS             164..478
                     /locus_tag="PLV81_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11517.1"
                     /translation="MNGDVLIQNIINLFVMAIILEAAIMAIFSMSAFRDMKMTRAVEA
                     TRDVIILLAAFFLCYKVDVLHVFRGTGLKIPVLFDIIISTLVLTRMTNFVRQVFNRLR
                     QD"
     gene            complement(644..943)
                     /locus_tag="PLV81_15360"
     CDS             complement(644..943)
                     /locus_tag="PLV81_15360"
                     /inference="COORDINATES: protein motif:HMM:NF015959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVG family protein"
                     /protein_id="HOM11518.1"
                     /translation="MNDIITEVRVFPKDNLGKTLGFANITLLDKFVVKNLRIVKGDKG
                     IFIGMPSNKRKNGEYIDLFFPITQEARDLITNAIIDKYNEVMNTSYSVDNKIKKS"
     gene            complement(1124..2551)
                     /locus_tag="PLV81_15365"
     CDS             complement(1124..2551)
                     /locus_tag="PLV81_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11519.1"
                     /translation="MSQNRGILFAIVNSIAIILIPLCIIVSIFYVLLTTPLFYTTILK
                     RIDIVGTFVKAKNLEITQSIQKEIDNKVGLASYVLTYQAAKKQYEEAKKAYDIINKTE
                     EYEKLQKQLDEIKNLSYSDVKQAFPNKQSFENNQKLELAKIQSQIESIEEYRNKYKAD
                     IAAAKEKLEEIEDTFEEAQDEYTEKQEEANEIIQKHQSTFASKLNDDLDVLKPVITDI
                     IHEKLIDGNIVPLIQKYITFFTSYNTIKSEYILELTDTSNPMYPKRVTQILLPDITIR
                     LWANENGIKKHILSDILVEEIKKTPNLKNRTFLIAVFTFADTAIAEFIGSSYLKKAGL
                     WFDNGVIYKHNIVLSGDTADTVITIIKIFSYGIYFVYATVILVIVYMLFIIFSSMDKI
                     KKFLWLKRVLMYPSAIILCIGVIGILLPIFFIHQSDTLSLIASQMLRELAFNISLCTL
                     TPIMVLFLVLLLCGLVFRKLYIIKL"
     gene            complement(2686..>2922)
                     /locus_tag="PLV81_15370"
     CDS             complement(2686..>2922)
                     /locus_tag="PLV81_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11520.1"
                     /translation="CFKIILHHSINTKSYHNHNLNNSVVMLLMSIKTCNSISIKVQCQ
                     HKITLTKNLYSAIFYYAVTIANTLLYGLMCDSNC"
BASE COUNT          901 a          485 c          494 g         1042 t
ORIGIN      
        1 actgggggcc acacccagaa atactaaaaa aatcacacgc cacccagcac ctgtaaccca
       61 ttaacaaatt attattgaca tctacattta tccatgtata caataaagaa gttgtaccag
      121 ggaatggtat atttttattt aaagaaaagg aggtcgtgca attatgaatg gtgatgtgct
      181 tatccagaac ataattaatc tttttgtcat ggcaatcata ttagaagcag ctataatggc
      241 cattttctca atgtctgctt tccgtgatat gaaaatgacc cgtgctgtag aagcaaccag
      301 agatgtgatt atattgcttg cagctttttt cctgtgctac aaagttgatg tgcttcacgt
      361 attcagaggc actggattaa aaataccggt tctttttgac attattatca gtacactcgt
      421 actgacgcgc atgactaatt ttgtgaggca ggtattcaat cgtttacgtc aggattagtg
      481 tcattggctt ttaagcaaaa gactacaaaa agcaatagtt ttttccggta actatcgcca
      541 ataaaagatt attagaaaat ttatgtatac catacaacac aaaaagccgc actagcagtg
      601 gtgtggctgc atatttacgg tttccgaagg gggctacata gtatcaggat tttttaatct
      661 tattatccac tgagtagctg gtattcatca cttcgttata tttgtcaatt attgcatttg
      721 tgatgaggtc ccgtgcttcc tgagtaatgg ggaagaacag gtcaatgtac tcaccatttt
      781 tgcgcttgtt ggatggcata ccaataaaga tgcctttgtc cccttttaca atgcgcaaat
      841 tcttaacaac aaacttgtcc aacaaagtga tgttggcaaa ccccagagtt ttccctaaat
      901 tatccttggg aaacacccta acttcggtaa taatgtcgtt catgtgatca ccaccatacc
      961 gtatgtataa ttttttaaat tatacaatgt ggccttagta tacgtcaagc aaaataattt
     1021 tttgtatttt tgtattataa tattacttca ttcaggcctg aaaatactca aaatttctta
     1081 atgatgcctc atatattatg aggcgatagt tatggttaaa aaatcatagt ttaataatat
     1141 atagttttct gaataccagt ccacacagta ataacaccag gaataaaacc atgattggtg
     1201 tcagagtgca caatgatata ttgaatgcca gttcacgtaa catctgagat gcaatgagcg
     1261 ataatgtatc agactgatgg atgaaaaaaa ttggcaatag tatgccaatg actccaatgc
     1321 aaaggattat agccgaagga tacatcaata cccgtttaag ccataagaat ttctttattt
     1381 tatccattga tgaaaagata ataaatagca tatatactat taccagaata acagtagcat
     1441 acacaaaata tattccgtat gaaaaaattt ttataatagt tattactgtg tcagcggtat
     1501 cacctgatag tactatattg tgtttatata ttaccccgtt atcaaaccac aaccctgctt
     1561 tttttagata gctactccca ataaattccg caattgcagt atcagcaaat gtaaatactg
     1621 ctattaaaaa agtcctgttt ttgaggtttg gcgttttttt aatttcttct accaggatat
     1681 cgctcaggat gtgttttttt atgccatttt catttgccca caaacgtata gtgatatcag
     1741 gtaaaagaat ttgggtaacc ctctttggat acatcggatt gctggtatcg gtcagttcca
     1801 gtatatattc agatttgatt gtgttatacg atgtaaagaa ggtaatgtat ttttgtataa
     1861 gtggaacaat gttaccatca ataagttttt catgaatgat gtctgtaata acaggcttta
     1921 gtacatctaa gtcatcattg agctttgaag caaatgtgct ttggtgcttt tgtattattt
     1981 catttgcttc ttcctgcttt tcggtatatt catcctgtgc ttcttcaaat gtatcttcaa
     2041 tttcctcaag tttttctttt gctgcagcaa tatcagcttt atacttattg cggtattctt
     2101 ctatggattc aatctgactt tgaatttttg ccagctctaa tttttgatta ttttcaaatg
     2161 attgtttatt ggggaatgcc tgttttacat cactgtagga taaatttttg atctcatcaa
     2221 gctgtttttg tagtttttca tattcttcag ttttatttat gatatcatat gcttttttgg
     2281 cttcttcata ttgttttttt gctgcctgat atgtcagtac atatgatgca aggccaacct
     2341 tattatcaat ttctttctga atggattgtg taatttcaag attttttgct ttaacaaagg
     2401 tgccaacaat gtctatgcgt ttaagaattg tagtgtaaaa caaaggtgtc gttaacagca
     2461 catagaatat tgatacaatg atacataaag gtataaggat tattgctata ctgttaacaa
     2521 ttgcaaatag aattcccctg ttttgtgaca tgcctgccct ccagctgtaa aagaatttgt
     2581 tttattatta tcccaacgaa atctattttt tatagtaatt atctaccaag catactacat
     2641 caagtttatg tattacatag tattaatgct gcataatcaa ctgtgtcaac aattactatc
     2701 gcacattaaa ccatacagta atgtattggc gatagttact gcatagtaaa aaattgcaga
     2761 atataaattt ttagtcaatg taattttatg ttgacactgg acttttattg atattgaatt
     2821 acacgtctta atactcatga gtaacatgac aacggagttg tttagattgt ggttgtggta
     2881 actttttgta ttaatactat ggtggaggat aatcttgaaa ca
//
LOCUS       DAORYL010000176         2907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_22764_length_2907_cov_5.017882, whole genome shotgun sequence.
ACCESSION   DAORYL010000176 DAORYL010000000
VERSION     DAORYL010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22764_length_2907_cov_5.017882"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="PLV81_15375"
     CDS             <1..1186
                     /locus_tag="PLV81_15375"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOM11521.1"
                     /translation="VITGFFGIALLPIDICSFKLFPYRIFFILLLPLFFIRILFNEKL
                     SLFNSGIKWYLIFLIFWLGYSFISLTWAISKANAIKDIVFLFMGIALIFFICLYIRNK
                     KDFMNIYYLCFVAFICLILIGFWEVITGNHLLTSRFFDSPLRFIPTSTFHNQNDFATF
                     LALYIPFGIGIFYYAHSLVLRFLGIMSISGAVYLILVTKCVSGILAVVLELIVLSVLY
                     IFNKRKFFLLAVLIPVVGIIVVWQYPFIHQYIQQMASVGERINLIRNGIHFLCCTFGF
                     GVGAGNIEYWMVNYPMYDVKVLNIHNWWAEILAEYGIFVFAGYVLFYFDIIYQLIKTY
                     LSTTSCFMKLLCTTLILSLCGFFVACIGPSSFLNITAQWYIFGIALAFLKHITSTEEQ
                     NG"
     gene            1179..2312
                     /locus_tag="PLV81_15380"
     CDS             1179..2312
                     /locus_tag="PLV81_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019558158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOM11522.1"
                     /translation="MADYICQMTNRIKVAHLSSAHPPADTRIFYKECRTLAQAGYEVV
                     LIIPHDRDEIVNGVRIRAVSKPTSRFDRMMSTVREVYRAALEEDAIIYHFHDPELIPV
                     GILLKNKGKKVIYDVHEDLPRQVLTKPWIKPFLRRVVGAGAQAVEKFCAHYFDRIITV
                     TSTIAERFPENKTTIVHNYPILEEFAGTDFIPYQKRNPLVVYVGGITVIRGIKEMVNA
                     MNLIPETLGARLVLAGKFSPPALENDVSSMAGWSNVEYLGWQSRDEVADLLSKARIGL
                     VLLHPTLNYMDAFPIKMFEYMAAGIPVVASNFPLWRKIVSEIGCGLVVDPLNPQAIAD
                     AVQWLLAHPVEAELMGKRGQDAVIKKYNWHTEAQNLLDDIRVW"
     gene            2336..>2907
                     /locus_tag="PLV81_15385"
     CDS             2336..>2907
                     /locus_tag="PLV81_15385"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOM11523.1"
                     /translation="MKILILTQYFPPEVGASQTRLYETVKALQRCGQDVEVFTAMPSY
                     PRGEIFDDYKGCFYKREEIYGIKVYRVWSYASNDLGQVKRLLSYSSFTGLALTALTAV
                     RPRPDLLFVESPPLTLGITGYLLSKVWRCPWILNLSDLWPDSIFALGSMDEESFAGKS
                     LLKLESFLYKKATGITAVTEGMISDLRLKK"
BASE COUNT          815 a          464 c          631 g          997 t
ORIGIN      
        1 agttattacc ggtttttttg gaatagcatt actcccaata gatatatgtt cttttaaact
       61 ttttccatac cggatttttt ttattttgtt gttgcctctc ttctttatac gcattttatt
      121 taatgaaaaa cttagcttat ttaacagcgg tattaagtgg tatctcatat tcctcatctt
      181 ttggctaggt tatagtttta tctccttaac ttgggctatt tctaaagcaa atgctattaa
      241 ggatatagtt tttctattca tgggaattgc actaattttt tttatctgcc tttatatacg
      301 aaataagaaa gatttcatga atatttacta tttgtgtttt gtcgcattta tctgtttaat
      361 acttattggt ttttgggagg ttattactgg taatcactta ttaacgtccc ggttttttga
      421 tagtccactt cgttttatac cgacaagcac atttcataac caaaatgatt ttgcaacttt
      481 tttagcttta tatattcctt tcgggatagg aatattttat tatgcacaca gtttagtact
      541 taggttttta ggtattatga gtatctcagg tgcagtatat ctaatactgg taacaaaatg
      601 tgttagtggt attttagcag tggtattgga attaatagtt ttatcagtgc tatacatatt
      661 taataaaaga aaattctttt tgcttgcggt tctaatcccg gtggttggaa ttattgtagt
      721 ttggcaatac ccatttattc atcaatatat acaacagatg gcttcagtgg gtgaacgtat
      781 caatttaata cgcaatggca tccattttct ttgctgtaca tttggtttcg gtgtgggtgc
      841 tggcaatatt gaatattgga tggtcaatta tccgatgtat gatgtaaaag ttttgaatat
      901 acacaactgg tgggcggaga ttttagcaga gtatggtatt tttgtctttg caggatatgt
      961 tttattttat ttcgatataa tctatcaact cataaaaact tatttaagta ctacaagctg
     1021 ctttatgaaa cttttgtgca caacattgat tttatcgttg tgtggttttt ttgttgcttg
     1081 tattgggcca agcagttttt taaatattac tgcccagtgg tatatttttg gtatagcact
     1141 tgcttttttg aaacatatta catcaactga ggagcaaaat ggctgattac atttgtcaga
     1201 tgactaatag aataaaagtc gcacatctat cttctgctca cccacctgct gacacccgta
     1261 tattttacaa agagtgcagg acattggctc aagcaggata tgaggttgta ttgatcattc
     1321 ctcatgatag agatgagatt gtaaatggtg tccgaatcag ggcagtatcc aaaccaacca
     1381 gtcgttttga tagaatgatg agcacagtga gagaagtgta cagagcagcc cttgaagagg
     1441 acgcgattat ttatcatttt catgatcctg aactgatacc ggtgggtata ctgctaaaaa
     1501 ataaagggaa aaaagttatt tacgatgtac atgaagatct gccgcggcaa gtcctaacaa
     1561 agccgtggat aaaacctttt ttaagacgtg ttgtgggagc cggtgcccaa gctgtagaaa
     1621 aattttgtgc ccattatttt gatagaataa taactgtgac atcaacaata gcagaacgct
     1681 ttccagaaaa taaaaccact atagtgcaca attatcctat ccttgaagaa tttgcaggta
     1741 ctgattttat accctatcag aagcggaatc cgttagtggt ttatgtaggt gggataacag
     1801 taatccgtgg tattaaggaa atggtgaatg ctatgaattt aatacctgag actttagggg
     1861 caaggcttgt gcttgccggg aagttcagcc ctcctgcttt ggaaaacgat gttagttcta
     1921 tggctggttg gtctaatgtt gagtatttag ggtggcagtc gcgggatgaa gttgcggatc
     1981 ttttaagcaa agctcgtata ggattggtgt tgctgcaccc taccttaaat tacatggatg
     2041 ctttccccat taaaatgttt gaatatatgg ctgccggaat accagtggtg gcatctaatt
     2101 ttcctttgtg gagaaaaatt gtcagtgaga ttggctgtgg gttggtggtc gacccactta
     2161 accctcaggc tatagctgat gctgtacagt ggttgctggc tcatcctgtt gaggctgaac
     2221 tgatgggtaa acgtggacaa gatgcagtga ttaagaaata caattggcat acagaagctc
     2281 agaacttgct ggatgatatc agagtctggt aaaatagaga gtaggatgat gcagcatgaa
     2341 gatattaata ttgacgcaat attttccacc tgaggtaggc gccagccaga cacgcttata
     2401 tgaaacagta aaagcacttc agcgctgtgg acaagatgtt gaggttttta cagctatgcc
     2461 ctcttacccg cggggtgaga tttttgatga ttacaaaggc tgtttttata aaagagaaga
     2521 gatatatgga attaaggtgt atcgagtttg gtcctatgcc agcaatgacc tggggcaagt
     2581 aaagaggctt ctttcataca gcagctttac aggattggct ttgactgctc ttactgctgt
     2641 gaggcctcgg ccagatctgt tatttgtgga atcacctcct ttgacattgg gtatcaccgg
     2701 ttatttactc agcaaagtat ggagatgccc ctggattttg aacttgagtg acttatggcc
     2761 tgattctatc tttgcgttgg ggtcaatgga tgaggaatct tttgcaggta aaagcctgct
     2821 gaaacttgaa agttttttat acaaaaaagc tactggaatt actgcagtta cagaaggtat
     2881 gatttcagat ctgaggctta aaaagaa
//
LOCUS       DAORYL010000177         2865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_23221_length_2865_cov_15.034520, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000177 DAORYL010000000
VERSION     DAORYL010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23221_length_2865_cov_15.034520"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            321..1511
                     /locus_tag="PLV81_15390"
     CDS             321..1511
                     /locus_tag="PLV81_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HOM11524.1"
                     /translation="MSDIVIKKSYEEINAKIKKGDAVVVTAEEMAGIVKSEGYKSAFK
                     KVDVVTTGTFGTMCSSGAFINFGHTRPKIKASKVWLNDVEAYAGLAAVDIYIGATQVQ
                     EDDPLNKVHPGRFKYGGGHVIEDLIAGKPVKLKAKSYGTDCYPLREYEATVTIHDLRD
                     AFLYNPRNAYQNYNCAVNQSKKTIYTYMGILRPNMGNATYSSAGQLSPLLNDPYYWTI
                     GVGTKIFLGGTVGAVTWRGTQHDPNAPRGDNGVVRSGAGTIAVTGDMKQMSAQFIKGA
                     SITGYGCSLMVGLGIPIPMLNEDLAFFTGVSDDQIFCQVVDYGYDYPNAIGRVIAEVS
                     YAQLKSGFIEIGGKRIPTAPLSSYPMAKKIATILKDWIKASQFTLGQPQILLPSVPYD
                     KRHE"
     gene            1504..2226
                     /locus_tag="PLV81_15395"
     CDS             1504..2226
                     /locus_tag="PLV81_15395"
                     /inference="COORDINATES: protein motif:HMM:NF003323.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HOM11525.1"
                     /translation="MSNTLPYRTKLATRFTTFEVRVETTNLYICAHSNVYDRAIVLVK
                     SLREDIERYCEKHPQFLHALTPLEPEALAPEVVQRMCKASLLAGVGPMAAVAGAIAQM
                     VGEDLLHYSPEVIVENGGDIWAYVREPAKISVFAGRSAFSKHLGIALTPDMTPCGICS
                     SSGRFGRSFSFGKADIATIIADTAALADAVATATCNMVQSDSDCTQAVSFATSISGVK
                     GALVIYWDTMAVKGSIELTEIE"
     gene            2239..2685
                     /locus_tag="PLV81_15400"
     CDS             2239..2685
                     /locus_tag="PLV81_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOM11526.1"
                     /translation="MESRNVLLIFNQHIMYKPIIYRIAKDFNVIFNVLEAKILPKQEG
                     RLILQLSGEKQDLDACITYLKDEEVVVDILADKIQRDEDRCVHCGACTAVCRTDALVI
                     NRETMMVEFHPDRCVACGLCKLACPVKAMSGISIDVDSEELAVKAV"
BASE COUNT          811 a          610 c          657 g          787 t
ORIGIN      
        1 cctcttccag caatttattt atttttattc ttgcttttgc ttctttttct gccatcacaa
       61 agatctttta caatatatca taattgaatt catcttttta agtttcacca tttgtacata
      121 gacacaaatt ttcatatatc tgcaatttct atgtcgttaa atataaaatt tttattacat
      181 tttctttttt tcaagccttt tttaattttt attaaacatg caagctcctc atcctttcaa
      241 tcataaattt ttacttgcca gttttcaagc tctggctcca taatggtatc ccacacatta
      301 taacacaaag gaaatccacc atgagtgata ttgtcataaa gaaaagctat gaagaaataa
      361 acgcaaagat aaaaaaaggt gatgcagttg ttgtcactgc tgaagaaatg gcaggtatag
      421 ttaaatctga aggatataaa tcagcattta aaaaagttga tgttgtaaca accggtacct
      481 ttggcaccat gtgttcttct ggtgcgttta ttaattttgg ccacacccgc cccaagatta
      541 aagcatcaaa ggtgtggctt aacgatgttg aggcgtatgc tggccttgcc gcagtggata
      601 tttacattgg cgcaacacag gtacaggaag atgacccact caataaagta catccaggac
      661 gctttaaata tggtggtgga catgtcattg aggatttaat tgccggcaaa cccgtaaagc
      721 ttaaagcaaa aagctatggt acagattgtt atcccttacg cgaatatgaa gcaacagtaa
      781 ccatacatga cctgcgtgat gcatttttat acaatccccg caatgcctat caaaactata
      841 actgtgcagt taaccagtcc aaaaaaacta tctacacgta catggggata ctgcgcccca
      901 atatggggaa tgcaacttac tcaagtgccg gccagctaag ccccctgctc aatgacccgt
      961 attactggac aattggtgta ggtaccaaga tatttttagg cggtactgtt ggtgctgtaa
     1021 catggcgcgg tactcagcat gaccccaatg caccccgcgg cgataatggt gtggtgcgat
     1081 caggtgcagg caccattgct gtaaccggcg atatgaaaca gatgtcagcg caattcatta
     1141 aaggtgcatc aattaccggg tatggctgct cgcttatggt tggacttggg attcctattc
     1201 ccatgttaaa cgaggacctg gcattcttta ccggcgtgtc cgatgaccag atcttctgcc
     1261 aggttgtgga ctacggctat gattacccca acgcaatagg acgagtcata gccgaggtaa
     1321 gttacgcaca gctaaaatca gggtttattg aaattggcgg caaacgtatt ccaaccgcac
     1381 cactttcaag ctaccccatg gcaaaaaaga ttgctacaat tcttaaagat tggataaaag
     1441 caagccagtt taccttaggt cagccacaaa tacttctgcc atcggtaccg tatgataaac
     1501 gccatgagta acacattgcc ctaccgaaca aagcttgcca cacggttcac cacctttgag
     1561 gtacgtgttg aaacaaccaa tctttatata tgcgctcatt ccaacgtata tgatagggcg
     1621 atagttcttg tcaaaagctt acgggaagat attgagcgct attgtgaaaa acatccacag
     1681 tttcttcatg ctctgacccc gcttgagcct gaggcattag cgcctgaggt tgtgcaacgc
     1741 atgtgcaaag cttcgttgct ggcaggagtt ggtcccatgg cagcagtcgc gggcgctatc
     1801 gcccagatgg tgggagaaga cttgctgcac tatagccctg aggtcattgt tgaaaacggc
     1861 ggcgatatat gggcctatgt gcgtgaacca gctaaaatta gcgtttttgc ggggcgctca
     1921 gcgttttcaa aacatttagg tattgctctg acccctgata tgacgccctg tggcatctgt
     1981 tcatcatcgg gcaggtttgg ccgctcattt agctttggca aagccgatat tgccacaatc
     2041 attgctgata ctgccgcact ggccgatgcc gtagccaccg ccacatgcaa catggtacag
     2101 agcgatagtg actgcacaca agctgtatcg tttgcaacca gtatatctgg agtaaaaggc
     2161 gcactggtga tatactggga tacaatggca gtcaaaggta gcattgaact tactgaaatt
     2221 gaataaacga ggtttaccat ggaatcacga aatgtactgt taatttttaa tcagcacatc
     2281 atgtacaaac caatcatata tcgtattgca aaagatttta atgtaatctt caacgtgctt
     2341 gaagccaaaa tattacccaa gcaggaagga aggcttatat tgcagctatc aggcgaaaaa
     2401 caggacctgg atgcctgcat aacctacctt aaagatgagg aagttgttgt agacatcctt
     2461 gccgataaaa ttcaacgtga cgaagatcgc tgtgtgcact gtggcgcctg caccgctgta
     2521 tgccgcaccg atgcccttgt tataaaccgt gaaaccatga tggtggaatt tcatcccgac
     2581 cgttgtgtag catgcggttt atgcaaactg gcctgccccg tcaaggcaat gagcggcata
     2641 tccatagatg tggattcaga ggaattagca gtaaaagccg tgtaggtacg gttttatacg
     2701 attgttatat tgaacggata tgcacacggg tgtagggacg gttcgtggaa tggtgttgtt
     2761 atcgggtgta tgaaaacggt gttgttaacg gatatgcaaa tgggtgtagg gacgggtgta
     2821 gggacgggtg tagggacggg tgtagggacg ggtgtaggga cgggt
//
LOCUS       DAORYL010000178         2857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_23306_length_2857_cov_14.356888, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000178 DAORYL010000000
VERSION     DAORYL010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23306_length_2857_cov_14.356888"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="PLV81_15405"
     CDS             complement(<1..131)
                     /locus_tag="PLV81_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020603177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11527.1"
                     /translation="MKQYENINKVAKITLLFWLMKILVTTLGETLGDFISMTLNLGY"
     gene            396..1403
                     /locus_tag="PLV81_15410"
     CDS             396..1403
                     /locus_tag="PLV81_15410"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOM11528.1"
                     /translation="MPNLRQNHVQATPSVRHQFNKVYHLYFSSKIQIKMKGNEISVKE
                     LRADAIACLTKLQQILLVKEYGKGTVRNYCQEMKLLFKYYNHKAVADIRQTDIEQYLQ
                     YIKDVHKVGRAKCRSVAQACSFFFKRVMPSEYIVPSNLYPRKQFILPNIMSEEEVKRL
                     FAAPLSVKEYCVVGLLYGCGLRISEVSNLRIIDIESDNKRIKVYQGKGGKDRYTLLPE
                     HLLLKLRELYISEGRPKEYLFTSKQTKRAIHLRSTQVIVNSAMAKAGFKDKHFTAHTL
                     RHSFATHMLNCGNNIHVIKTLLGHSKIETTMVYLHLQQHTQAGIQSPLETVVYGTAAT
                     R"
     gene            1381..2598
                     /locus_tag="PLV81_15415"
     CDS             1381..2598
                     /locus_tag="PLV81_15415"
                     /inference="COORDINATES: protein motif:HMM:NF033538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HOM11529.1"
                     /translation="MEQQPLADVRQLLQTKIFAHPSASGFNSYSRAVLSKLSRCHTSG
                     IGVHQYRCSNEGCSHVHYQYHSCGNRHCPNCGGTKREQWIEDRMGELLPTTYFHIVFT
                     IPQELRSLCMGNRQRMFGLLFKSAQHTLLTLAKDDRYIGAVPGIVSILHTNGQDLNFH
                     PHVHNIVSGGGISKDGKWIKEKRSNGKFLFPRRSMEKMYKGYFMWQLRKYITSGELKY
                     EDKEALENIVSTVGKKKWNVYAKAPFGGPAQIVEYLGRYTHKVAITAHRIISIDDNNI
                     SFRYKDYQDGNKQKVMTLSHVEFLRRFEQHILPKGLVKIRHSGYLHSKDKMKRIAEVC
                     RQLQLPTPMPRVAIPATVRLMLQTGKDVTVCPVCRIGKLQLVRTMIFYNGRLIDVLQL
                     RNRGSPRIKKQPA"
BASE COUNT          928 a          596 c          629 g          704 t
ORIGIN      
        1 aaataaccta aattcagtgt cattgaaata aaatcaccca aggtttctcc aagagtggtt
       61 accaaaattt tcattagcca aaagagtaaa gtgattttgg ctaccttgtt tatattttca
      121 tattgtttta tttcctttat taatgatcat taagtttcaa cactattata atttcatcat
      181 atataatact tcaaggttct tggttaatgg gtattaagta aaatcgcatt aaacgaacag
      241 ggctttgtgc aggttgggaa atggaattcc gtctgctcgt agctgaaagt gacaaatgca
      301 atattgccga tgttttattc ataagctaaa tttaatttat attgccctgc tggggctgaa
      361 gccaagtaga aaaacaaaag atgagttttt tccgtatgcc caacttgcgc caaaaccatg
      421 ttcaagcaac accttcggtg agacatcagt tcaacaaggt ttatcatctt tacttttcat
      481 caaaaataca aataaaaatg aaaggtaacg aaatttctgt aaaagaactc agggcggatg
      541 caatagcatg cttgacaaaa ctacaacaga tcttgttggt aaaagagtat ggcaagggta
      601 cggtgcgtaa ctactgccag gagatgaagc ttctgttcaa gtattacaac cacaaagcgg
      661 tggcagacat ccggcaaacg gatattgaac agtatcttca atacatcaaa gacgtacata
      721 aagtaggcag ggcaaaatgc cgcagtgtgg cacaggcttg tagtttcttt ttcaaaaggg
      781 taatgccttc ggagtatatc gtaccctcca atctgtatcc gcgcaagcag tttatccttc
      841 ccaatatcat gagtgaagaa gaagtgaaaa ggctgtttgc agcgccatta agcgttaaag
      901 aatactgtgt ggtaggtctg ctatatggtt gtgggctacg catcagcgaa gtaagcaatc
      961 tacgaataat agacattgag agtgataaca aacgcatcaa agtatatcag ggcaaaggtg
     1021 gcaaagatcg ctacacactg ctaccggagc atctgttatt gaagctcaga gagctgtata
     1081 tatcagaagg ccgccccaag gagtatctgt tcaccagcaa acaaaccaag cgggccatac
     1141 atctgcgaag cacacaggtg atagtcaaca gcgccatggc caaagccggg tttaaggaca
     1201 aacactttac ggcacatacg ctgcgccaca gtttcgctac ccacatgctc aactgcggca
     1261 acaatatcca tgtcataaaa accctgctgg gacatagtaa aatagaaacc accatggtat
     1321 atcttcacct gcaacaacac acgcaggcag gcatacaatc acctttggag acagtggtgt
     1381 atggaacagc agccactcgc tgatgtccga cagttgttgc aaacgaaaat ctttgcacat
     1441 ccatcagcaa gtggcttcaa ttcctacagt cgggctgtat tgagtaaatt gagccgctgc
     1501 catacatcag gcattggggt gcatcagtat cgttgcagca acgagggttg cagccatgta
     1561 cactatcagt atcatagctg tggcaatcgg cattgcccta actgtggggg caccaagcgc
     1621 gaacaatgga tcgaagaccg catgggcgag ctattgccca ccacttattt tcatatcgta
     1681 tttactatac cacaggagtt aagatcactt tgcatgggca atagacaacg gatgtttgga
     1741 ttgttgttca aatcggcgca acacacgctg cttacgctgg ccaaagacga cagatacata
     1801 ggagccgtac cgggtattgt aagcatcttg cataccaatg gacaagacct caactttcac
     1861 ccgcacgtac acaatattgt gagtggcggc ggcattagta aggatggtaa atggatcaaa
     1921 gaaaagcgta gcaacggaaa gttcttattt ccacgtaggt cgatggaaaa gatgtacaaa
     1981 ggatatttta tgtggcagct gagaaaatat attaccagtg gagagttaaa atatgaagac
     2041 aaggaagcac tggaaaatat agtttccacc gtaggcaaaa agaaatggaa tgtatatgcc
     2101 aaagcaccct ttggcggccc ggcacagata gtggaatacc tgggaagata tacccataaa
     2161 gtggccataa ctgcacatcg tatcataagt attgatgaca acaatatcag ttttcggtat
     2221 aaagattatc aggatggtaa caagcagaaa gtaatgacac tcagtcatgt cgagttttta
     2281 agacgatttg aacagcatat actgcctaag ggtttagtaa aaatccgcca ttcgggttat
     2341 ttgcacagta aggataagat gaagcggata gcagaggtgt gcagacaatt acaactgcct
     2401 acacccatgc ctcgggtagc catacccgcc accgtgaggc tgatgctaca aaccggaaaa
     2461 gacgtgacag tatgtccggt atgccgaata ggaaaactac aattggtacg taccatgatt
     2521 ttctacaacg gacgtctcat agatgtcctg caactgcgca atcgtggctc accaaggata
     2581 aaaaaacaac cagcatgatg agtagaaata gtaaaaatac aaaccataac caattgtctg
     2641 aaaacaagta tcgtttttgg gtaggggcag ctgtgtacga acgggtaaag aatttagcaa
     2701 aatgtatgca aatactaagc aagatccatc catctaatcc tccagctact cacaaaacca
     2761 cctaagagaa aacgccccaa gcccggtgtc gctgaacacc ggattgcaaa agccccgttc
     2821 gtttatgatc cggggctttg gttttttaag gtacctc
//
LOCUS       DAORYL010000179         2660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_25733_length_2660_cov_7.269098, whole genome shotgun sequence.
ACCESSION   DAORYL010000179 DAORYL010000000
VERSION     DAORYL010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25733_length_2660_cov_7.269098"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="PLV81_15420"
     CDS             <1..674
                     /locus_tag="PLV81_15420"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOM11530.1"
                     /translation="TKAIMLNYPSNPTGASISKEHLEAIAAIAIANNLIVISDEIYDA
                     ITYNGEHVSIISIPGMAERTIYLNGFSKAYAMTGWRLGYACGPEPIISAMNKIHQYTA
                     LCAPSIAQYAALEALQRGQRDVASMRSQYLKRRNFITARLNEMGLPCLPPKGAFYAFP
                     DISSTGLSSQEFALKLLKSHKVAVVPGTAFGSSYDNHIRCAYATSMEQLKEAMDRMED
                     FVKGL"
     gene            868..1575
                     /locus_tag="PLV81_15425"
     CDS             868..1575
                     /locus_tag="PLV81_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11531.1"
                     /translation="MKLFKIIIYSILTFIISSSLYASDLGIGVSGWYADWKMYNPDNP
                     NDKTNKMDPAFCIGPSVSYQFAQSWSTTLVALFTPSPYEMSNESSDATKIRRYDSDLV
                     LNYQLVRYLKIFFGGKYLGFTFKDGKHHGAGPGGGIGLTIPVIDNFYFLGNVSGLYLW
                     GSHKDNDSSKDFTEYGYNAAVQLAYYASSAGLTTSIGYRYQFIKSRYDTDDPYGKKDE
                     HTFKGFTFLIVKSFHWE"
     gene            1609..2328
                     /locus_tag="PLV81_15430"
     CDS             1609..2328
                     /locus_tag="PLV81_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11532.1"
                     /translation="MRKNKIYIMFLILFVASKIYATDLGVGISAWYADWKMESKTLGT
                     FNLEPALFVGPTISYQFEEKWSITLIALATVNKYDQDISANDSVKYRRYDVDCTLNYQ
                     ISRYIKCFAGIKYLAFDFNSDNYNGINHGVGPGAGIGFSSPIVSSIYLLGIVSGIYGY
                     GLETTDNPPAGSPDNRNFYSFGINSSLQLAYYIQAIETTISAGYRYQYIQLHYLDYGT
                     DEDSKYDNHFRGFTASVAKSF"
     gene            2478..>2660
                     /locus_tag="PLV81_15435"
     CDS             2478..>2660
                     /locus_tag="PLV81_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11533.1"
                     /translation="MKKYFILFMIVVFLNSMLYAADLGVGISAWYADWKMERKSSTVG
                     GTVNMDEVLYIGPSIAY"
BASE COUNT          833 a          484 c          507 g          836 t
ORIGIN      
        1 aaacaaaagc cataatgctt aactaccctt caaacccaac cggcgcatct atcagcaaag
       61 aacatttaga agcgatagct gccattgcta ttgccaacaa cttaatcgtt atttcagatg
      121 aaatctatga tgccattaca tacaatgggg aacacgtttc aatcatatcc atacctggaa
      181 tggctgaacg cacaatttac ttaaacggct tttccaaagc atatgccatg accggatggc
      241 gcctgggata tgcatgcggg cctgaaccca ttatcagcgc aatgaacaag attcaccagt
      301 atacagcact gtgtgctcca tccattgcgc agtatgctgc attggaagcc ttacaaaggg
      361 gccagcgcga tgttgcatca atgcgttcgc agtatcttaa acgtcgaaac ttcatcactg
      421 cacgcttaaa cgagatggga ctaccctgcc ttccacccaa aggtgccttt tacgcctttc
      481 ctgatatatc atccacaggg ttatcatcac aggagtttgc acttaaactt ttaaaaagcc
      541 ataaggttgc tgttgtgcca ggaactgcgt ttggaagctc gtatgacaat cacatacgct
      601 gtgcctatgc aacatccatg gaacagctca aagaagccat ggaccgtatg gaagattttg
      661 tgaagggatt gtaaccagtt acagtaacaa aaaaatgtac aaacataaaa atatgaaaat
      721 gcattgacat tttcatttat atgttttact aaaaaaatga tagagttcgc catttctgtt
      781 accaggcata cattatacta tctttatgta tcataatctt atcccatacc atagactttg
      841 tatactataa ttatctggag gtaatacatg aaattgttta aaattattat ctatagtatt
      901 ctgacattta ttatttcctc gtcactgtat gcatcagatt taggtattgg cgtatcaggg
      961 tggtatgctg actggaagat gtacaatcca gataatccaa atgataaaac aaataaaatg
     1021 gaccctgctt tttgtattgg cccttctgtt tcgtatcagt ttgcccagtc ctggagtacc
     1081 acactggtgg cattgttcac tccatcacca tatgaaatgt caaatgaatc cagcgatgca
     1141 acaaaaatca gacgttatga ttctgattta gtattaaact atcaattagt gagatacctg
     1201 aaaatattct ttggcggcaa atatttgggt tttacattta aagatgggaa acatcatggt
     1261 gcaggacccg gtggtggcat tgggttaacc attccagtta ttgacaattt ttattttctt
     1321 gggaatgttt caggcctgta tctgtgggga agccacaaag ataatgatag ctcaaaagat
     1381 tttaccgaat atggctataa tgcagcagta cagttagcgt actatgcttc gtcagcaggt
     1441 ttaaccactt caattggcta tcgatatcag tttataaaat ccagatacga tactgatgat
     1501 ccttatggca aaaaggatga gcatacattt aaaggattta cgttccttat agtaaaatcg
     1561 tttcactggg aatagatttt aaattttatt acatttgagt tttttattgt gagaaaaaac
     1621 aaaatataca tcatgttttt aatactgttt gttgcatcaa aaatatatgc tactgacctg
     1681 ggtgttggta tatcagcatg gtatgccgac tggaagatgg aaagcaaaac gttaggtact
     1741 ttcaatcttg agccagcttt atttgtgggc ccaactattt cttatcagtt tgaagaaaaa
     1801 tggagcatca cgttgattgc tcttgcaacc gtaaataaat atgatcagga tatttctgca
     1861 aatgattctg taaaatacag gcgatacgat gtagattgta cccttaacta tcaaatttcc
     1921 cgatatataa agtgttttgc aggaataaag tatttagctt ttgattttaa ttctgataat
     1981 tataatggca taaatcatgg agttggacca ggtgcaggca ttggcttttc atcccctatt
     2041 gtttcaagca tataccttct tggcatagta tccggtatct acgggtatgg gctggaaaca
     2101 actgacaatc ctcctgccgg gagcccggac aatagaaatt tttattcatt tggaattaac
     2161 tcttcccttc aactggcata ttacatacag gctatcgaaa caacaatttc agctggatat
     2221 cgttatcaat acattcagct tcattacctg gattatggaa cagatgaaga tagcaaatat
     2281 gataatcatt ttcgagggtt tacagcatca gttgctaaat cattctaggt ttatcgtaat
     2341 atacaagaat tattatgtag tatagaatat tgaatactta caagttattt agtatgtgct
     2401 tttacaatca taagaatggc tgtagaatcc cttcaaggat attaaaacaa aaaattattt
     2461 tttggaggtt ataaactatg aagaagtatt ttatactgtt catgatagtt gtattcctga
     2521 attccatgtt atatgctgct gacctgggtg ttggcatatc agcatggtat gctgactgga
     2581 agatggaaag aaaatccagc actgtgggtg gcacagtgaa tatggatgaa gtactgtaca
     2641 ttggtccttc tattgcatat
//
LOCUS       DAORYL010000180         2645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_25941_length_2645_cov_10.535135, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000180 DAORYL010000000
VERSION     DAORYL010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25941_length_2645_cov_10.535135"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..>2645
                     /locus_tag="PLV81_15440"
     CDS             95..>2645
                     /locus_tag="PLV81_15440"
                     /inference="COORDINATES: protein motif:HMM:NF015497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HOM11534.1"
                     /translation="MRKVVLIALVCNMFFSACGTDTHTENAVNTPDVPVRVYATGYTV
                     TDGEGNAVTSADFLNLFDNRIETAWVINTKHIVAAECNNTYIDTVRIYKAEGGMFTLY
                     ADTMNSHIATINESTSNDNGWIDIPVKRTLNKLILVIEPITVAQIAHVELWGVQSQLC
                     GIYTQYRFADTDAVHNTYGTAIDVYDAAIEDNETVEGKVTVPNPWQYRRAYIQMLAGG
                     AFRPVSVAVKVNECDWLGGLTIPEEAGMGDDVREHTIEVNPQWFVPGQNSVYIQTRGG
                     IVTVKKLRVIAVKDTGFNELQRNDKVLYDGNTATYETVGNEGAIVTGDRKMRIYGALV
                     YMHEGAVTLQAQTERGWVNIAGVVAKESGWYYSACEKAIVTDTLRVVAAQDKAVTVGE
                     VALCASPAEEQVPEIVISYPRNGEYFGRTAHIEGFVHPYATQDGVAGLTVNGRELTCG
                     IAGGFTAAISKNMVGLGQEDDSKPWQLTFVAEYGRSGVQAVERVYSSSTIRKVQTTLV
                     LDDGFMPYTQGSGGGITSPAPVSYPGSDVPSGTGLTVTVAPDSAKSVYYQGVQLEIPK
                     GAVDETVTVTIIPLGEHELAPLTPGMRNVTAPAAGYRFLFNGKPHGSFKKAITISIPY
                     DSGKLLSGTSEKQIQMYYYDDGAAVWKRMEGSSAVTKKDPDYSLYARSVISSTGASGV
                     VVAKSTHFTDFINATLTVPEHPEALLFNPNSIKDIKAGDPVSGMQLIQPPQVSNTGDA
                     GIRFPITVPKGRLGVQPQLELSYSQQGGNGPFGLGWNMRISSISIDTRWGAARYDGTD
                     VFSLDGSQLVPVDNGGNYRTRIEGGFAKIYYDKQSNCWTVTHKNGVRYIYGE"
BASE COUNT          813 a          472 c          691 g          669 t
ORIGIN      
        1 ttgtggtaat aattgttaaa ccaattgtga acaatcatac ataattcatt actattatga
       61 agatatataa taaaaacata cagggaggaa atgcatgagg aaggtcgtcc ttatagcgct
      121 ggtgtgtaac atgttctttt cagcatgcgg tactgatacg catacagaaa acgctgtaaa
      181 tacaccagat gtcccggtac gtgtatatgc aacagggtat acagtaactg acggagaagg
      241 taatgcagta acatccgctg attttctgaa cctttttgat aacaggattg aaacagcctg
      301 ggtaataaat acaaagcata tagtagcagc agaatgcaat aatacctata tagatactgt
      361 tcgcatatat aaagcagaag gtggaatgtt taccctttac gctgatacca tgaattccca
      421 cattgccact attaatgaat caacttcaaa tgataatggt tggattgata ttccggtcaa
      481 gcgcaccctt aacaaactga tacttgttat agagccgata acagtagcac aaatagccca
      541 tgtagagttg tggggtgtgc agagccagct ttgtggtata tatacgcagt acagatttgc
      601 ggatacagat gcagtgcata atacctatgg aacagctata gatgtatatg atgcagcaat
      661 tgaagacaat gaaactgtag aagggaaagt gacagttcct aatccctggc aataccgtcg
      721 tgcatatata cagatgcttg ctggcggtgc tttcaggccg gtaagtgtgg cagtaaaggt
      781 aaatgaatgt gactggcttg gagggcttac catacctgaa gaggcgggta tgggagatga
      841 tgtacgtgag cataccattg aagtaaatcc acagtggttt gtcccgggtc aaaattcagt
      901 gtatatacaa acaagaggag gcatagtaac ggtaaaaaaa ctgcgtgtta ttgcagttaa
      961 ggataccggt tttaatgagc tacagcgtaa tgacaaagtt ctgtatgatg gtaatactgc
     1021 aacttatgaa acggttggga atgagggggc gatagtaact ggtgacagaa agatgcgtat
     1081 ttatggagca cttgtatata tgcatgaggg agcagtgacg ctacaggcac aaacagaacg
     1141 tggatgggtt aatatagcag gagtggtagc taaagaaagt ggctggtatt acagtgcatg
     1201 tgaaaaagcg atagtgaccg atacacttcg ggttgttgca gcgcaggata aagcggtaac
     1261 tgtgggagaa gttgcactat gtgcatcacc ggcagaagaa caggtgcccg agattgttat
     1321 aagttatcca cgcaatggtg aatattttgg gcgtacagca catatagaag gttttgtaca
     1381 tccatatgcc actcaggatg gagtggcagg gcttaccgta aatggcagag agcttacctg
     1441 tggtattgct ggtggattta ccgctgccat aagcaaaaat atggttggcc ttgggcagga
     1501 agatgacagt aaaccctggc agttaacgtt tgttgctgag tatggaagat caggagtgca
     1561 ggctgttgaa cgagtatata gcagtagcac aatcagaaaa gtccagacaa cactggtgct
     1621 tgatgatggc tttatgccgt atacacaggg gagtggtggt ggaataactt caccagcgcc
     1681 ggtaagttac ccgggaagcg atgtgccttc aggaactggc ttaacagtaa cagtggcacc
     1741 cgatagcgcc aagagtgtat actatcaggg agtgcaactg gaaataccaa aaggagcggt
     1801 tgatgaaact gttacggtaa ccattatacc gctgggagag catgaacttg caccacttac
     1861 accgggtatg cggaatgtga cagcgcctgc tgccggatac cgctttttgt ttaacgggaa
     1921 acctcatggt agctttaaaa aagccataac cataagtatt ccgtatgaca gtggtaagct
     1981 attaagcggt accagtgaga aacagataca aatgtactac tacgatgatg gagcggctgt
     2041 atggaaacgt atggagggaa gtagcgcagt aacaaaaaag gatcccgatt attcactcta
     2101 tgctcgtagt gtcataagct ctaccggtgc atccggggtg gtggtggcaa aatcaacaca
     2161 ctttacggat tttattaatg caacgttgac agtaccagag catcccgaag cccttttatt
     2221 taatcccaat agtataaaag atattaaagc tggtgatccg gtaagtggaa tgcagcttat
     2281 acaaccacct caggtaagta ataccggtga tgctggaatc cgtttcccta ttaccgttcc
     2341 caaagggcga ttgggagttc agccgcagct tgaattaagc tatagccagc agggaggaaa
     2401 tggtcccttt gggttggggt ggaatatgcg catttcatca atcagcattg ataccaggtg
     2461 gggtgctgct cgctacgatg gtacggatgt gtttagtctt gatggcagcc agcttgttcc
     2521 ggttgataat ggcggtaact atcgcacacg cattgaagga ggttttgcaa aaatatatta
     2581 tgacaaacag agtaattgct ggacagtgac acacaaaaat ggggtgcggt atatatatgg
     2641 tgaaa
//
LOCUS       DAORYL010000181         2559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_27214_length_2559_cov_8.985224, whole genome shotgun sequence.
ACCESSION   DAORYL010000181 DAORYL010000000
VERSION     DAORYL010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27214_length_2559_cov_8.985224"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            452..1156
                     /locus_tag="PLV81_15445"
     CDS             452..1156
                     /locus_tag="PLV81_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11535.1"
                     /translation="MLKEYFSTLQQRLGVKETKGGKKTAKKSSTKEEYNITKLINLLQ
                     DIVPNDFVISDKKPVDASGYVPKTVDYIAYFKKWNIIDQMMNGHIPSELVYGTFHVVD
                     CITPQQLPEILNNVALTKKLNKFSELEEADLIPAFIIAGTSDIDMPQLKNTILDYYIE
                     RSIDNTLEFDIMMIIGKGIIIKDWREKRKFVALETEEDTLLWFFILMNEYLDVDKKHS
                     LDLRNYVKETRRYREY"
     gene            1277..2383
                     /locus_tag="PLV81_15450"
     CDS             1277..2383
                     /locus_tag="PLV81_15450"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOM11536.1"
                     /translation="MKAKYTLFNVIIVCALCIGLCNAGAFAKSKDLLLVIDTSLSMAG
                     YGGKNIMPLVKQSLPKFIDQLDDNDSLTLVTFDTEVKIYPTVYIKHKSNKDILNKYIS
                     MVEAGGKWTYTMAMMTAVFDKARKLQAEDPDREQVIIIITDAIDDPPPYARAHRYDVK
                     KIAKQYSGNEWFIFFVNLGQMQKDSHIAKVQKEVKESISQYTKVIDAREGLDKIITQD
                     LKKDIDTMAKVKEINTRPFYMHPIFIIVVAIVLLIILLLLLRRAAQLKVRGALEYWNN
                     DLLEPTIEKVNLTAKNSRTISIGKDSHNLIRIHDFTLRTPILLKAVWFDKAVKCQLIA
                     PENAVQFVNRDAGDILNDGDIFKIANYTFKYYEG"
BASE COUNT          909 a          384 c          492 g          774 t
ORIGIN      
        1 aacaaaagat gaggatttag ggcttgaaga agacgaagag gttagtggtt cagtctcagg
       61 tgaatttgat gacgttaacg tagctgaatt ttgattttaa tattatataa gtatagtgta
      121 aaggcgaacg tatgttcgct tttatgctat aattcattgc cataccatta atatggtttg
      181 acaaaaatag tgtagttgcg atacaaacta aatgtagatg agttaaccat agctgtaatt
      241 ccaactatcc aaactgttga gtatgagagg gactcagcat gtcatcctga taaaaacgaa
      301 gagtctctgc aaaaagcaat ggagaagatt ctttggtact atcgcacatc agaatgacca
      361 ttgatgttaa aaaaatttct catccccata ggttggggtt acatgcaatg atagatagta
      421 ctaaattcta ttttcaatgg agaaacaaat aatgttaaaa gaatattttt caacgttgca
      481 gcagcggctg ggtgttaagg aaacaaaagg tggcaaaaaa acagcaaaga agtcatctac
      541 taaagaagaa tataatatta caaagcttat aaatcttttg caggatattg ttcctaatga
      601 tttcgtaata tcagataaaa agcctgttga tgcaagcggt tatgtcccca aaaccgtgga
      661 ttacattgca tatttcaaaa aatggaatat aattgatcaa atgatgaatg gccacatccc
      721 ttcggaatta gtatatggca cttttcatgt cgttgactgc ataactccac agcagcttcc
      781 tgaaatattg aataatgttg cgttgacaaa aaaacttaat aaattttcag aactggaaga
      841 agcggattta atacctgcat ttatcattgc aggcacaagc gatattgata tgcctcaatt
      901 aaaaaataca atactggatt actatattga gcgcagtata gataatactc ttgaatttga
      961 tatcatgatg attataggaa agggcataat tattaaagac tggagagaaa aacgaaaatt
     1021 tgtagcactg gagactgaag aagatactct tttgtggttt tttatcctga tgaatgagta
     1081 tcttgatgtt gataaaaagc attcgcttga tttacgaaat tatgtaaaag aaacacggag
     1141 atatagagag tattaaaata ttttaaataa taaaaattaa ttttacttga ctaatgtttt
     1201 tttatcattt agcttttaac caagatttat tgtattttcc agatagataa attttatata
     1261 gtataaggag ttcgccttga aagcaaaata tacactattc aatgtaataa ttgtttgtgc
     1321 tctttgtatt ggtttgtgca atgcaggcgc ttttgcaaaa tcaaaggatt tgctccttgt
     1381 tattgacaca tcattatcaa tggctggcta tggtggcaaa aatattatgc cgctggtaaa
     1441 gcagagcttg cccaaattta ttgatcagct tgatgataat gacagcctga cacttgttac
     1501 atttgatact gaagtcaaaa tctatccaac tgtctatatc aagcataaaa gcaataaaga
     1561 catactgaat aaatacatat ccatggttga agctggtggc aagtggacat ataccatggc
     1621 tatgatgacg gccgtgtttg acaaggccag gaagctgcag gctgaagacc ctgacaggga
     1681 gcaggtcatt attataatca ctgatgccat agatgaccca ccaccgtatg cacgggcaca
     1741 tcgttatgat gtcaaaaaaa ttgcaaaaca atattcgggt aatgaatggt ttatattttt
     1801 tgttaattta ggtcagatgc aaaaggacag ccatatagcc aaagtccaaa aagaggttaa
     1861 agaaagcatt tcccagtata ccaaggtgat tgatgcacgt gaagggcttg acaagataat
     1921 tacccaggac ctaaaaaaag atatcgatac catggcaaag gtaaaggaaa ttaatactcg
     1981 tccattttat atgcacccta tttttattat agtagtggcg atagttcttt taattatcct
     2041 gttgctgctg ttgcgccgtg cagctcagct taaggtaaga ggtgcacttg aatactggaa
     2101 taatgatctt ctggagccga caattgaaaa agtaaacctt actgcaaaaa acagcagaac
     2161 catatctata gggaaagata gccataattt aataagaatt cacgatttta cactgcgcac
     2221 gcctatattg cttaaagcgg tatggtttga taaagctgtt aaatgccagc tgatagcacc
     2281 agaaaatgca gtgcagtttg tcaacaggga tgcaggcgat attttaaacg atggtgacat
     2341 tttcaaaata gctaactata cctttaaata ttatgaagga taaaaagctg taataattaa
     2401 aaaattttta ataaattttt aaataaagcg ttgacaagca tactgatatt aatttaaata
     2461 ttacattcaa atgctttgtg ctgaagatag ggtatttcaa catagcatac ggtgcatcag
     2521 tagagtataa ctatatatta tgcatctttg acaaaaatg
//
LOCUS       DAORYL010000182         2456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_28694_length_2456_cov_8.049563, whole genome shotgun sequence.
ACCESSION   DAORYL010000182 DAORYL010000000
VERSION     DAORYL010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28694_length_2456_cov_8.049563"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PLV81_15455"
     CDS             complement(<1..523)
                     /locus_tag="PLV81_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOM11537.1"
                     /translation="MNLEKSYPYKRVLITGSGSGLGRALAIEFAKRKWNVIVADINFQ
                     RASDTAQLIEKYGGKSIPKKCDVTVENDCINLLTEVKKHYKGCDILINNAGVAAAGFF
                     EKIPLKTWDWIYAINTKSIILMCRTFIPLFKEQKYGYIVNIASNAGIASLPEMACYNM
                     TKAATISLSETLSV"
     gene            complement(586..1689)
                     /locus_tag="PLV81_15460"
     CDS             complement(586..1689)
                     /locus_tag="PLV81_15460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOM11538.1"
                     /translation="MNFHNTCNSQIAEFQDVAKIFSNRRLLNNIEFNDEYPFSNLIMD
                     YIDDMASLGFFSINLPQEFGGIDCERDVISALCLEIAQVDASIATIILTNASALSILN
                     QDRKDGDTILPELKHIGSKPIAFQLYSHIDALSLPEYTDGKVNGMAKMVSCAPIADYA
                     ILPVKYNHSVSYCCINIHDTGVTISDPIVTLGLHACPVANVYCNSCNARIIGLATRGH
                     HYFSVMYSDMALCASSILLGIMKGSFKQALAYTLERHQGGRAIIHWPQVRMMLSEMKL
                     AIDSAETSFHYLMNFQLSTALYEQYVQSFFLTLSNSAIITTSIGIQLLGGNGYTKDYG
                     QEKRLRDARQAKSFYGMHSLKKMNFINDYIKRM"
     gene            complement(1708..>2456)
                     /locus_tag="PLV81_15465"
     CDS             complement(1708..>2456)
                     /locus_tag="PLV81_15465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOM11539.1"
                     /translation="STASELIFEDCFVPNEWVTMRAQDPVIKNSKKGPFWYTHTLVDY
                     VTSSSRAGVGAIATGIARSSYERTKQYASKKIVNGEKLINHQWAQILLTEMYRNVNIA
                     RLTYMESVYTDMLQGLFKLLNAPFLYLPLTYAPKVFFSLLSPLLNLQIVTWFLRKYYY
                     DWYTDNERNNSTGWASVAKITCSEIGFINTNIALDLTGDDGLCHELGIEKTFRDIKLL
                     EIYESTNQINAMNICHSMVTNDCNDIKYYA"
BASE COUNT          776 a          440 c          407 g          833 t
ORIGIN      
        1 ccacactcaa cgtttcgctt agagatattg tagctgcttt cgtcatattg tagcaagcca
       61 tttcgggaag ggaagcaatg ccagcatttg aagctatatt gacaatataa ccgtattttt
      121 gttctttaaa caatggaata aacgttctac acatcaaaat aatactttta gtgtttattg
      181 cataaatcca atcccatgtt ttgagtggga ttttttcaaa aaaaccagca gctgccactc
      241 cggcattatt aatcaagatg tcacatcctt tatagtgttt tttaacttct gtaagtagat
      301 ttatacaatc attttcaaca gtaacatcgc acttttttgg aatagatttc cctccatatt
      361 tttctattaa ttgtgcagtg tctgaagctc tttgaaaatt aatatcagcc acaattacgt
      421 tccatttgcg ttttgcaaat tctatagcta aggcacgccc taaaccactc ccggaacctg
      481 tgataagaac tcttttataa gggtaagatt tttctaaatt catatgcaaa cctctattat
      541 tacaaacttg tatcgaaaat cttgtaatcg ctattgatta cattattaca ttctttttat
      601 ataatcattt ataaaattca ttttttttaa tgaatgcatg ccataaaaac ttttagcctg
      661 acgagcatca cgtaatcgtt tttcctggcc ataatcttta gtatatccat tacctcccag
      721 aagttgaatg ccaattgaag ttgttataat tgcgctattg gacaacgtta agaagaaaga
      781 ttgtacgtat tgttcatata aagctgttga aagttgaaag ttcattaaat aatgaaagga
      841 tgtttccgca ctatcaatag caagtttcat ttcggaaagc atcattctta cctggggcca
      901 gtgaattatt gctctgcctc cctgatgacg ttccagagta tatgctaaag cttgtttaaa
      961 tgaacctttc ataataccta atagaataga cgaagcacac agtgccatat ctgagtacat
     1021 aacagaaaag taatgatgtc ccctggttgc cagaccaata attctggcat tgcaactgtt
     1081 gcaataaacg tttgcaacag ggcaggcatg taatcctaat gtaacaatag gatcagagat
     1141 ggtgacaccg gtatcgtgaa tattgataca acaataggat accgagtggt tatactttac
     1201 aggcagtatg gcataatcag caatgggtgc acatgaaacc atttttgcca tcccattaac
     1261 tttaccatcg gtgtattcag gcaatgaaag agcatcaata tgactgtata gttggaaagc
     1321 gatgggtttt gatcctatgt gtttaagttc agggagtatt gtatctccat cttttctatc
     1381 ttgattaagt attgataatg cagatgcatt agtaagaata attgtagcaa tacttgcatc
     1441 aacctgtgca atttctaaac ataatgctga tataacatca cgttcgcaat caattcctcc
     1501 aaactcttgt ggaagattaa tagaaaaaaa acctaaggac gccatatcgt caatataatc
     1561 catgattagg tttgaaaatg gatattcatc attgaattct atattgttaa ggagacgtct
     1621 gttactaaag atttttgcca catcctggaa ttctgctatt tggctattac aggtattgtg
     1681 aaaattcatg gcaacctcct gtgtatatta tgcataatat tttatatcat tgcaatcatt
     1741 agtaaccatt gaatgacata tattcattgc atttatttga tttgtacttt catagatttc
     1801 tagtaattta atatctctaa aagttttttc aatccctaat tcatgacaga gtccatcatc
     1861 ccctgttaga tccagtgcaa tatttgtatt gataaatcct atctcactac aggtaatttt
     1921 tgcaactgaa gcccaacctg ttgaattatt gcgttcatta tcggtatacc agtcatagta
     1981 atattttctc aaaaaccagg ttactatttg taaattcaaa agaggcgata gaagtgaaaa
     2041 gaaaactttt ggagcgtacg ttaacggtaa gtataaaaag ggtgcgttaa gcaatttaaa
     2101 taatccttgc aacatatcgg tataaacact ttccatataa gtaagtctgg ctatatttac
     2161 attgcgatac atttcggtta ataatatttg agcccattga tgatttataa gtttttcacc
     2221 atttactatt ttttttgaag catattgttt tgtacgttca tacgaactgc gtgcaattcc
     2281 ggtagctata gcaccaactc cagcgcgcga acttgatgtc acataatcta caagagtatg
     2341 tgtgtaccag aatggccctt ttttagaatt tttaattact ggatcctgtg cacgcatggt
     2401 gacccattca ttgggtacaa aacaatcctc aaaaattagt tcactagctg tagatg
//
LOCUS       DAORYL010000183         2455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_28715_length_2455_cov_4.791667, whole genome shotgun sequence.
ACCESSION   DAORYL010000183 DAORYL010000000
VERSION     DAORYL010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28715_length_2455_cov_4.791667"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PLV81_15470"
     CDS             complement(<1..523)
                     /locus_tag="PLV81_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOM11540.1"
                     /translation="MHLEKEYPEKRVLITGAGSGLGRALSIEFAKRKWNIIVADINSN
                     RATETVQLVDRYGGKGFAIKCDVTSEKDCVALLNTIYKNYGGCDILINNAGVAAAGFF
                     EKIPVTTWDWIYSINTKSIILMCRTFIPMFKEQRYGYIINIASNAGIASLPEMACYNM
                     TKAATISLSETLSV"
     gene            complement(581..1690)
                     /locus_tag="PLV81_15475"
     CDS             complement(581..1690)
                     /locus_tag="PLV81_15475"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOM11541.1"
                     /translation="MNTDTYKDEIIQFQDMAKMFSTKRLLPFVEQNDEYPFSVAEIDY
                     IEDMSSLGFFSINLSHEYGGIGCGSDVIATLCLEIAQVDASIATIILNHASAITILNM
                     AAYDGDATLSEIINAGSKPVAFQLYSHINAMSLPVYDNGIINGIAKTVVCGNIADYAI
                     IPVNNNNSISYCCVKIDDNAVIVSEPIVMLGMHACPVTDLHFNNSKASIIGNAEKGSF
                     YFEAMYARMALCAAAIIAGIMKGSFKQALIYTQQRQQGGRAIIHWPQIQMMLSEMKTA
                     IDYAECCLQYLIPQEKSINTHLHELYTKSIFLTLSEMAISTTSIGVQLLGGNGYTKDY
                     HQEKRLRDARQAKSFFGMHSLKKIDYINQYLKSIE"
     gene            complement(1707..>2455)
                     /locus_tag="PLV81_15480"
     CDS             complement(1707..>2455)
                     /locus_tag="PLV81_15480"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOM11542.1"
                     /translation="STASELIFEDCFVPNEWVSMRVQDPGIKNSKKGPYWYTHTLVDF
                     VTSSSRAGVGAIATGIARSSYERTKKYVTDKIANNDRLINHQWVQILLTEMYKNVNIA
                     RLTYLESVYTDMLQGLFKLLSKPPVHITVTYLPRFIFTLLSPLLNLPVVTWFFRKYYY
                     DLYTDTERNNSTGWASTAKITCSELGNRNANIALDITGDDGLRHEYGIEKTFRDIKLL
                     EIYESTNQINAMNICHAMVTNDCNDVIYYK"
BASE COUNT          776 a          452 c          386 g          841 t
ORIGIN      
        1 ccacactcaa cgtttcgctt agagatattg tagctgcttt cgtcatattg tagcatgcca
       61 tttcaggaag tgaagcaata ccagcattgg aagcaatatt aataatatat ccatatcttt
      121 gttctttaaa catgggaatg aaagttctac acattaaaat aatgctttta gtattaatgc
      181 tgtatatcca gtcccatgtt gtaacaggta ttttttcaaa aaaacctgca gcagcaacac
      241 ctgcgttatt tatcaaaata tcacagccac catagttttt ataaatagta ttaaggaggg
      301 caacacaatc cttttcactg gtaacatcac attttattgc aaaacccttt ccaccatatc
      361 tatcaaccaa ttgtacagtt tcagtagccc tgttactatt aatatcagct actattatat
      421 tccatttacg tttagcaaat tctattgata aggctctgcc caaaccgcta cctgcgccgg
      481 ttataagaac tcttttttca ggatattcct tctctaaatg cacattttcc tccaatttaa
      541 ttttaatgat taagaggtga aaaagcacta tgtagtatat tcattctatt gattttaaat
      601 attgattaat ataatcaatt ttttttaggg aatgcatgcc aaaaaaactt tttgcttgtc
      661 gtgcatctcg taaacgtttt tcctgatgat agtcttttgt atatccattt ccacctaaaa
      721 gttgtactcc tattgatgta gtagaaatag ccatttcact taatgttaaa aatatggatt
      781 ttgtataaag ttcatgcaaa tgagtgttga tagatttttc ttgtggaatt aaatactgta
      841 agcaacattc tgcataatca attgcagttt tcatctctga aagcatcatc tgaatctggg
      901 gccagtgtat gattgctctt cctccttgct gtcgctgttg tgtatatatt agtgcttgtt
      961 taaatgagcc tttcataatc cctgctataa tagcagcagc acatagagcc atacgtgcat
     1021 acattgcttc aaagtagaaa ctcccttttt cagcgttgcc aataattgaa gctttactat
     1081 tattgaaatg taaatcagta actggacatg catgcattcc taacataacg ataggttctg
     1141 agacaataac tgcattatca tcaattttaa cacaacaata ggaaatagaa ttgttattgt
     1201 tcacgggaat aatagcataa tcagcaatat taccacaaac aactgtcttt gctattccat
     1261 ttattatgcc attgtcatat actggtagtg acatagcatt aatatgacta tataattgaa
     1321 atgcaacagg ttttgaacct gcatttatta tctctgacag tgttgcatcg ccatcataag
     1381 ctgccatatt taaaatagtt atagctgatg cgtgattaag aatgatggtt gcaatactgg
     1441 catcaacctg agcaatttcc aaacataatg tagctataac atcactacca caaccaatac
     1501 ctccatattc atgggaaaga ttaattgaaa aaaatcccaa tgaactcata tcctcaatat
     1561 aatcaatctc agcaacggaa aaaggatatt catcattttg ttcaacaaaa ggtagaagac
     1621 gtttagtact aaacatttta gccatatctt gaaattgtat tatttcatct ttatatgtat
     1681 cagtattcat ggcgacctcc ttttaattat ttataataga taacatcatt gcaatcattt
     1741 gtaaccatag catggcagat attcattgca tttatctggt tggtactttc ataaatttcc
     1801 agtaatttga tgtccctgaa agtcttttct ataccatatt catggcgaag gccatcatca
     1861 ccggtaatat ccagtgctat attggcattt ctattaccta attcactgca ggtaattttt
     1921 gccgttgatg cccatcccgt ggagttattg cgttcagtat cggtatacaa gtcataatag
     1981 tattttctaa aaaaccatgt aacaacaggt aaatttaata ggggcgatag caaagtaaat
     2041 ataaatcgag ggagatatgt tactgtaata tggacagggg gtttgctgag caatttaaat
     2101 agtccttgaa gcatatctgt ataaacactt tccaggtatg ttaatcgagc aatattaaca
     2161 tttttataca tttcggtaag gagtatttgt acccactgat ggttaatgag cctatcatta
     2221 tttgctattt tgtctgttac atatttttta gtgcgttcat acgagcttcg cgctatacct
     2281 gtcgcaatag caccaacacc agctcgtgag cttgaggtta caaaatcaac cagtgtgtgt
     2341 gtataccagt agggtccttt tttagaattt ttaattcctg gatcctgaac tcgcatagaa
     2401 acccattcat tgggtacaaa acaatcctca aaaattagtt cactagctgt agatg
//
LOCUS       DAORYL010000184         2408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_29454_length_2408_cov_9.158521, whole genome shotgun sequence.
ACCESSION   DAORYL010000184 DAORYL010000000
VERSION     DAORYL010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29454_length_2408_cov_9.158521"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLV81_15485"
     CDS             <1..236
                     /locus_tag="PLV81_15485"
                     /inference="COORDINATES: protein motif:HMM:NF021501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HOM11543.1"
                     /translation="YDGILYMCAILTLKVGAKEPDIHKRIENFERTKRYLSKLFGSGK
                     ASKSKPGPLLDMTRDLYDKMNEMLDQWKVGEQE"
     gene            212..994
                     /gene="truA"
                     /locus_tag="PLV81_15490"
     CDS             212..994
                     /gene="truA"
                     /locus_tag="PLV81_15490"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOM11544.1"
                     /translation="MESRGTRVRYALIVQYDGTAYSGFQIQDNANSIQHEIEKALKIF
                     TRQNVRIVAAGRTDAGVHALGQVVHFDLDTHVPADRLCISLNGILPADIAVKKAFVVD
                     NLFHARYSAIAREYLYVIYNHPYKSPFMRYRAMWVREPIDIEFCRKAATYCIGEKDFA
                     SFCKKTSAEESTVRYIEYITVERMFDDVVVFTIKGNAFLHNMVRILVGTILQLMKEGK
                     SPDTMQTILKQCDRTYSGITAPAYGLYLKKVYYPEKYNFLNV"
     gene            1182..1751
                     /locus_tag="PLV81_15495"
     CDS             1182..1751
                     /locus_tag="PLV81_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11545.1"
                     /translation="MKLKLSWLFVAFTLLCSANDVFAQRKKADYFLKPQTGIWFGPVT
                     PVFSTADDVDTALWGGGFVRFNILSLPLKIGFDSSYEHFKSEGVKELTVIPVYGNILW
                     RLPFDMPLAFQLKAGAGSAKVTIEPEGLQQWDPLGMLGFELSFPAGKWINIGLRIDYL
                     FIYEGYIQGASKNGHIINTGLTLYLNIGM"
     gene            1759..>2408
                     /locus_tag="PLV81_15500"
     CDS             1759..>2408
                     /locus_tag="PLV81_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11546.1"
                     /translation="MKKFFYYIVLFVAIVTVYGCTGKNALFDELSGSRLTVIIKGTFE
                     STSDSTSVNGWNTTVPGTKIDDSVNDFVTDSSESFPTTFMVDIAEIRLAKPDGSSDDK
                     VGNYRQVFTSQIDDSDPFFNGTGIKLKCDDIKPGKQYGMVKLYIRKLIFDNATQWYID
                     GSNNIVQDNAPEVIFHEEEVNGFDFNQLMVNTYYDYLKENYQDINRVFPLEIPIDGG"
BASE COUNT          743 a          407 c          554 g          704 t
ORIGIN      
        1 gttatgatgg catactgtat atgtgtgcaa tacttacgtt gaaggtgggt gcaaaagaac
       61 ctgatataca taagcggatt gaaaattttg agcgcacaaa gcgctattta agcaaactgt
      121 ttggcagcgg taaggcgtca aaaagtaagc cgggcccgct tcttgacatg acccgtgatt
      181 tatatgacaa gatgaacgag atgcttgacc aatggaaagt cggggaacaa gagtgaggta
      241 tgcgcttatt gtacagtatg atggtactgc ctacagtggt tttcaaatac aggacaatgc
      301 caattccatt caacatgaaa tagaaaaagc attgaaaatt tttacacgcc agaatgtacg
      361 gattgttgca gcaggaagga ctgatgctgg cgttcatgca ttggggcagg ttgtacactt
      421 tgatcttgat actcacgtgc ctgctgacag gttatgcata agccttaatg ggatattgcc
      481 ggctgatatt gcagtaaaga aggcgtttgt agttgataac cttttccatg cacgttacag
      541 tgccattgcg cgagaatatc tgtatgtcat ttataatcat ccgtacaaaa gcccgtttat
      601 gcgctatcgt gcaatgtggg tgcgtgaacc tattgatatt gagttttgcc gtaaggctgc
      661 aacatactgt ataggtgaaa aggattttgc ttcattctgt aaaaaaacat cagctgaaga
      721 aagcactgtg cggtatatag aatatattac tgtagaacgt atgtttgatg atgtagtggt
      781 gtttactatc aagggcaatg cctttttgca taatatggta cggatactgg taggtacaat
      841 attgcagctt atgaaagaag gtaaaagccc tgacacaatg caaacaatac taaagcagtg
      901 cgacagaacc tatagcggaa taacagcccc tgcgtatggg ctgtatctta aaaaagtata
      961 ttatcctgaa aaatataatt ttttaaatgt gtaacaaaaa acacggtata tttccatatt
     1021 tttatactat gtatagtatg aatatatgaa aagaaaaaca tccccggctt gagaaatgaa
     1081 gtggatttac acgttcttat atacagtaag taaaaaataa ttattgacac atagtgatat
     1141 cacaacaatt gttagtgaaa caaaaagaaa aggtgatacg tgtgaagtta aaattatcgt
     1201 ggctctttgt tgcgtttaca ttgctatgca gtgccaatga tgtttttgca cagcgcaaaa
     1261 aggctgatta cttcctcaaa ccccagacag ggatctggtt tggtccggtt actccggtat
     1321 tttcaacagc agatgatgtt gatactgcgc tgtggggtgg cgggtttgtg cgatttaata
     1381 tattgtcgct gcctcttaag attgggtttg attcttcgta tgagcatttc aaatctgaag
     1441 gcgttaagga gctaacggta attccggtat acggcaatat tttgtggcgt ttgccatttg
     1501 acatgccgct tgcatttcag ctaaaggcag gtgcaggtag tgctaaggtt accattgagc
     1561 ctgaaggtct gcagcaatgg gaccctctgg ggatgcttgg atttgagcta tcgtttcctg
     1621 ccggcaaatg gattaacatt ggactcagga ttgattatct tttcatctat gaaggctata
     1681 tacaaggtgc cagcaaaaac gggcacatca ttaataccgg attgacactt tatctgaata
     1741 ttgggatgta atataacgat gaaaaaattt ttttattaca tagtattatt tgtggcgata
     1801 gttactgtct atgggtgcac cggcaaaaat gctctttttg atgagctgtc cggaagccgc
     1861 ctgacagtaa taatcaaagg tacatttgag tcaacaagtg atagcactag tgttaatggg
     1921 tggaatacaa ccgttcccgg aactaaaata gatgattccg ttaatgattt tgtaaccgat
     1981 agctctgaaa gctttcccac tacctttatg gttgatattg ccgagatacg ccttgcaaag
     2041 ccagatggca gcagcgacga taaggtagga aattaccggc aggtatttac atcacaaata
     2101 gatgacagtg acccgttttt caatgggacc ggtattaaac taaaatgtga tgacataaag
     2161 cccggaaaac agtacggaat ggtaaaactg tatatacgca agcttatctt tgataatgca
     2221 acgcaatggt acatagatgg atccaataat attgttcagg ataatgcacc tgaagttata
     2281 ttccacgaag aggaagtaaa tggttttgat tttaatcagc ttatggtaaa tacatactat
     2341 gattacctga aggaaaacta tcaggatata aaccgtgtgt tcccgctgga gatacctatt
     2401 gatggcgg
//
LOCUS       DAORYL010000185         2406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_29483_length_2406_cov_5.777967, whole genome shotgun sequence.
ACCESSION   DAORYL010000185 DAORYL010000000
VERSION     DAORYL010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29483_length_2406_cov_5.777967"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..543
                     /locus_tag="PLV81_15505"
     CDS             244..543
                     /locus_tag="PLV81_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11547.1"
                     /translation="MKRIIVIVSLILSLSVVVFAKEKSFTGRLIFIGKDYIEVKHGKT
                     EKIFYIQEKSVIMKNNTNVSFADLEACQVVKINYIAGDNKLFITKCEVVKESDCK"
     gene            557..2389
                     /locus_tag="PLV81_15510"
     CDS             557..2389
                     /locus_tag="PLV81_15510"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOM11548.1"
                     /translation="MQLTIDDHSIEIQYKTQDTSKTVSSHTIQHDNYLNDNLHFQYKR
                     NKDSIRCVVKPVTRLYIQKVILAFQVSPLDDIACFANGYQSWTYTGPFFKDSKKRKPW
                     PQFVIANQENAANPPKGKRGIVQSDMVFVLGDSNSQNAYVFGQLPVHRQFHQFVSFEY
                     NYRTKLLSIIWDVDRFCDAEEDYILDTVCIMQGNAWRILQTYAETIKQELSPTFNTTI
                     RKGWCSWYYYYNTITKDEILLNAKYAKEHSIPFDFIQIDDGYQTTIGEWTTVKGSFTA
                     AMRYIADTIKGYGFTPGIWYSPFIVSKDSRIYKENRSWILKDNNGKPVVAGFNPAWGG
                     YYYTVDVSQPAVLSWIEDTINTLLYEWGYEYLKLDFMYAAALPGVRHNTTVSRYEALA
                     QAVARIRKVSGNAFVLGCGMPLQAGIGYVDAMRIGPDVAPQWGRTFFDKLFNSDSGIS
                     TRSAICSSIYRSFMHNRFWVNDPDCLMIRQHKTKLNPEERQTLYNVITALGGMLVISD
                     RLPDYSATEREMLLQAIALFDKAKDGDIYCNAVLKPLRSFYNAKELGVLLNVDDTTCE
                     ATLEQEIINNYSKIFLIEKNTKIPSQTKNFDLIPHSSKLFLFEK"
BASE COUNT          812 a          373 c          467 g          754 t
ORIGIN      
        1 tttaaaaatg tggagagctt atactataaa gcttgtggaa gaacataaat atatcgatgt
       61 aaagcaaaat gtttattagt ttttgtcaag attaaaacac atcaatttta aaaaatattt
      121 ggactatatt ttagatttaa attataaaat gtagttgcag aaaattcaat tgtttttatt
      181 ctatttaatg aatatatata ctatacatgg catataatgt cgtttacaac aggaggttgc
      241 agtatgaaaa ggataatcgt tattgtatca cttattcttt ctctgtcagt tgttgttttt
      301 gcaaaagaaa aaagctttac aggccgcttg atatttattg gtaaagatta catagaggta
      361 aaacatggaa aaacagagaa aatattttat atacaggaaa aatccgtaat catgaaaaat
      421 aataccaatg tttcttttgc ggaccttgaa gcatgccagg ttgtaaaaat taattacata
      481 gcaggggata ataagctgtt tatcactaaa tgtgaagtag taaaagaaag cgattgcaaa
      541 taaaaaaggg tatataatgc agctaacgat tgatgaccat agcatagaaa tacaatataa
      601 aactcaggat acatctaaaa cagtatcaag ccatactata cagcatgata attatttaaa
      661 tgacaattta cattttcagt ataaacgtaa taaagattca atacggtgtg tagtaaaacc
      721 cgtaacgcgt ctgtatatac aaaaagtaat tctggcattt caggtttcgc ctttggatga
      781 tatagcatgt tttgctaacg gctatcagtc atggacatat acaggtcctt tttttaaaga
      841 ttcaaagaaa cgcaaaccct ggccacaatt tgtcatcgca aatcaggaaa atgcagccaa
      901 tccaccaaaa gggaagcgtg gtatagtcca atcggatatg gtgtttgtat tgggcgatag
      961 taactcgcaa aacgcttatg tttttgggca gcttcccgta cacagacaat ttcaccagtt
     1021 tgtttccttt gaatataact atcgcacaaa actgttgtcc attatatggg atgtcgatag
     1081 attttgcgat gctgaggaag actatatact tgatacagta tgtattatgc aggggaatgc
     1141 atggaggatt ttgcagacgt atgctgagac aattaaacag gaactatcgc ctacatttaa
     1201 taccacaata cgcaagggct ggtgttcctg gtattattac tataatacta ttaccaaaga
     1261 tgaaatactt ctcaatgcta aatatgctaa agaacattcc attccatttg attttatcca
     1321 gatagatgat gggtatcaga ctaccatagg tgagtggacc actgttaaag gctcatttac
     1381 ggctgccatg cggtatattg ctgataccat caaagggtat ggatttacac cagggatctg
     1441 gtattcgcca tttattgttt caaaagattc aaggatttat aaagaaaata gatcctggat
     1501 tcttaaggat aacaatggaa aacctgttgt tgctggtttc aatcctgcgt ggggtggtta
     1561 ttactataca gttgatgttt ctcagcctgc agtgttaagc tggattgaag ataccattaa
     1621 tacgttattg tatgaatggg gatatgaata tctcaaatta gattttatgt atgcagcagc
     1681 actaccagga gtacgacaca atactaccgt gtcacggtat gaagcgctgg cacaggcggt
     1741 tgcccgaata cgaaaagtaa gcggtaatgc gtttgtactg ggatgtggga tgcctttaca
     1801 ggcgggcatt gggtatgtcg atgcaatgcg tattggtcct gatgttgcac cgcagtgggg
     1861 gcggacattt tttgataaac tatttaacag tgattcagga atttcaacac gttcagctat
     1921 ttgtagcagt atttatcgtt catttatgca taaccgcttc tgggttaatg accctgattg
     1981 tcttatgata cgtcagcata aaacaaaact taatcctgaa gaacggcaaa cgctttataa
     2041 tgtaataaca gcgctgggtg gtatgcttgt aatatccgat aggttacctg attattctgc
     2101 tactgaacgt gaaatgctat tacaggcaat tgcattattt gacaaggcaa aggatggaga
     2161 tatttattgt aatgctgtat tgaagccatt acgttcattc tataatgcaa aagaattggg
     2221 tgtattatta aatgtagatg atactacttg cgaagcaaca cttgaacagg aaataattaa
     2281 taattattct aaaatattct tgattgaaaa gaatacaaaa ataccctccc aaacaaaaaa
     2341 ctttgattta atacctcaca gttcaaaatt atttcttttt gaaaaataat tttgcaaccg
     2401 tttact
//
LOCUS       DAORYL010000186         2400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_29598_length_2400_cov_14.409382, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000186 DAORYL010000000
VERSION     DAORYL010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29598_length_2400_cov_14.409382"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            331..618
                     /locus_tag="PLV81_15515"
     CDS             331..618
                     /locus_tag="PLV81_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11549.1"
                     /translation="MTITQHCVKQMHNRGITNTMVELVQLFGYQVGDRLIMDKKQIQE
                     LLDEINELRHQLLKMYEKGGVAVVEKNGALITAFAIQKRIGKIKKQYKHAG"
     gene            686..1756
                     /gene="cas1"
                     /locus_tag="PLV81_15520"
     CDS             686..1756
                     /gene="cas1"
                     /locus_tag="PLV81_15520"
                     /inference="COORDINATES: protein motif:HMM:NF013984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HOM11550.1"
                     /translation="MADIYLISDYGKLYKNNRVFNFLYPDGTVSKFFPHNTQRVFIIG
                     NIEITPEAFKMLMHNKIEVVFLNKNGLFNGKLVFDDSKNVLLRKKQYDKLNNDAFILG
                     WCKNIIEGKIRNQMVFANRIKRKHKETTQLQDYVNSMKDIIEKLETATTTNEARGYEG
                     AASRMYFGAIKYAILPHWAQFNGRSMNPPRDNVNAVLSFTYTLLNHLIESYILSEGID
                     TYVGYLHTVEYGRKSLIFDLMEEFRSPVCDTLTVTLFNLGVLNPDDFRTIDFSSSDDE
                     YPLEPMEEEEDNVSTRKGVLLTKDGLKKVIEKFEAKLEETYYYMPLQRQLSYRKIIAQ
                     QVAHCKRVINDEESQYKPFEVK"
     gene            1804..2028
                     /gene="cas2"
                     /locus_tag="PLV81_15525"
     CDS             1804..2028
                     /gene="cas2"
                     /locus_tag="PLV81_15525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOM11551.1"
                     /translation="MAKQLERMGIRVQYSFFNVELDDEMLDKLMQILLGLIDPKEDKI
                     YVYPLCTDCCKKVIIDGTGALLDLNTFIIL"
     gene            2064..2345
                     /gene="cas2"
                     /locus_tag="PLV81_15530"
     CDS             2064..2345
                     /gene="cas2"
                     /locus_tag="PLV81_15530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOM11552.1"
                     /translation="METWLVIYDIRNEKRLRRIAKLMERYGVRVQKSVFETMCDEHTI
                     QRMRHEAKMILEKEDSLIVIPLCAKCWQKKIQYGVKTEGIGEYKKFQVL"
BASE COUNT          875 a          338 c          492 g          695 t
ORIGIN      
        1 gtgataattt ccccaaatct caatgagtag cttcattgct gtctccagta gttaaatttt
       61 aatgttattg attgtggtac atacaaaagt ggaacattta tcagtaacta tcgcctataa
      121 aaaatattgt actggtgtag tggttggtca agtcattttt aatgtacaca tattggcgca
      181 gtatgttttg gtcaggattt ttaaaaaaat gtgtaagaaa gtgtagcatc taactcattc
      241 agtaactaaa tgaaaaattt aaaaaaaatg ccaataaatt ttaatactta acaatcaaat
      301 ataatagaac attaaaaggg agggatgcca gtgacaataa cacaacattg tgtaaaacaa
      361 atgcacaacc gtggtataac aaacaccatg gtagaactgg tgcaactctt tggctatcag
      421 gttggtgaca gacttataat ggacaaaaag cagatacagg agttgctgga tgaaattaat
      481 gaactgcgac atcaattgct taagatgtat gaaaaaggtg gagtggcagt ggtggaaaaa
      541 aatggagccc tgattacggc atttgccata caaaagcgga taggaaaaat aaaaaaacaa
      601 tacaaacatg ctggctaaac gtgtgcttgt gcagcttgca gggtaaaaac ctgtgggctg
      661 caaattattt ttataggagg ttgtaatggc tgatatatat ttaatctccg attatggaaa
      721 gctatataaa aataaccgtg tatttaattt tttgtatcct gatggaacag tgtcaaaatt
      781 tttcccccat aatacacaac gagtattcat cattggaaat attgaaataa ctcctgaagc
      841 atttaagatg cttatgcata ataaaattga agtggtgttt ttaaacaaaa atggattatt
      901 taatggtaag ctggtatttg atgatagcaa aaatgtatta ttgcgtaaaa agcagtacga
      961 taaactcaat aacgatgcat ttatacttgg gtggtgcaaa aatataattg aaggtaaaat
     1021 acgcaatcag atggtatttg ccaatcgtat aaaacgaaag cataaagaaa ccacacaatt
     1081 acaggattat gtcaactcaa tgaaggatat tatagaaaag cttgaaactg caaccaccac
     1141 caatgaggcg cgtggctatg aaggtgctgc atccagaatg tattttggtg caattaaata
     1201 tgcaatacta ccacactggg cacaatttaa tggccgaagt atgaacccac cacgcgataa
     1261 tgtcaatgca gtgttaagtt ttacctatac attattaaat catttaattg aaagctacat
     1321 actttcagaa ggtatagata cttatgtggg ttatttgcat acagttgaat atggccgcaa
     1381 atcgttgatc tttgatttaa tggaagaatt ccgatcacca gtatgcgata ccttaacagt
     1441 aacactgttt aatttaggtg tacttaatcc tgatgatttc cgtacaatag atttttcaag
     1501 tagtgatgat gaatatccgc tggaaccaat ggaagaagaa gaggataatg tttcaacgcg
     1561 taaaggcgta cttttaacaa aggatggttt gaaaaaggta atagaaaaat ttgaagcaaa
     1621 attagaagaa acatattact atatgccatt gcaaagacaa ctatcgtacc gtaaaataat
     1681 agcgcagcag gtagcacact gcaagcgtgt tataaatgat gaagaaagcc agtacaaacc
     1741 gtttgaggta aaatgaaata tttgatatgt tatgatattt gtgatcataa gaggttaact
     1801 cgtgtggcaa agcaattgga gcgtatgggc atacgcgtac agtattcttt ctttaatgta
     1861 gaactggatg atgaaatgct tgataagctg atgcagatac tactgggatt gatagatccc
     1921 aaagaagata aaatatatgt gtatccgcta tgtactgatt gttgtaaaaa ggttatcata
     1981 gatggaacgg gcgcactgtt agatttaaat acatttataa ttttatgaga tagttattga
     2041 caaatgaatt atagtatgat agtatggaaa cctggttggt aatatacgac attcggaatg
     2101 aaaagcgatt acgccgtatt gcaaaactaa tggaacgcta cggtgtacga gtgcagaagt
     2161 cggtctttga aacaatgtgt gatgagcaca caatacagag aatgcgccat gaagcaaaaa
     2221 tgatccttga aaaagaagat tcattaattg ttattccgtt gtgtgcaaag tgctggcaaa
     2281 agaagattca gtatggcgta aagaccgaag gaattggtga gtataaaaaa tttcaggttt
     2341 tatgaacagt tctttgaaaa ctgtataaaa taaggcatat tttgatgccg ccaacctaag
//
LOCUS       DAORYL010000187         2364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_30216_length_2364_cov_6.021654, whole genome shotgun sequence.
ACCESSION   DAORYL010000187 DAORYL010000000
VERSION     DAORYL010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30216_length_2364_cov_6.021654"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1029)
                     /locus_tag="PLV81_15535"
     CDS             complement(13..1029)
                     /locus_tag="PLV81_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11553.1"
                     /translation="MRCITAIKKIYAPIILCIFVSMLGCSGDSTFFSQRNDPEPEYRL
                     FDLLDEYPALYDAVSKLDQHTFNIMLADAVNADVLAVKEILPPTTDALPVLVDTIGLL
                     RQILYRIMHQEDYDWPQDTYTKYTEDLYSFLDSVSATSPGVSNEIVSLLRKLLGYIYY
                     AHGNEIETVMNDLNNFLQDTEGQNLQSVFPDLQEGLGKLLLRTNSTIQYNGDDLRLGN
                     AVEGVDAILSAVNDIATKDEEARDALYNTIRQAGALLTSKVGTKSFAQILQELLINAE
                     DYATIGGAVYSSDYNYHNDNAEFYVNTELSNGLRQFWPGIMTLFIKAKGEGGDFLKKS
                     QEIN"
     gene            complement(1181..>2364)
                     /locus_tag="PLV81_15540"
     CDS             complement(1181..>2364)
                     /locus_tag="PLV81_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11554.1"
                     /translation="FGFFTPKPVSTAVDFKPKDSVRTVANILTENTTAAADGIIPALA
                     NGKVVSKLLAMLQDVGQYGPSANPIYVDSDKTSSDYTQWGSRRKLFYGLEQIITRIKC
                     SQSTELSSGFSYFNISTTGWSYPGWIFTKDASLDVDLDIGLNEFIGTSDTDEIPETGE
                     KGLAVFVDNRPLNNSNPDRNWINFDKMFNGIAELLSTTGTTQGQYCITPDLISLLDKM
                     FTGVELEDKHLTALRHMLGVLLYKYDYSTSTWVVPADLKNLLTDNLPGILASYAGNYN
                     DLLSFVNDMLADDGFLEEFLQDFNTSYTAEELLQQIYDLLGDELITNPNSALWSNLGQ
                     ILTLLYINLDEAEKGVYQSQSDGSFDRFVYQTPYNGDAGDYIFSPDFNPYQTLGLILS
                     K"
BASE COUNT          741 a          499 c          386 g          738 t
ORIGIN      
        1 gacataattt agtcaattaa tttcttgact ttttttgaga aaatccccac cctcaccttt
       61 agctttaata aataatgtca tgatgccagg ccaaaactga cgcaatccat tggaaagttc
      121 tgtattaaca taaaactccg cattatcatt atgataatta taatcacttg aatataccgc
      181 acctcctatt gttgcataat cttcagcatt tattaataac tcctgtaata tttgtgcaaa
      241 actcttggtt cccactttgc ttgttaacag tgcgcctgcc tgacgtatgg tattatataa
      301 tgcatcgcga gcctcttcat ctttggttgc aatatcattc actgccgaga gtattgcatc
      361 aacaccttca acagcatttc ccaaacgcag atcatcacca ttatactgta ttgtagaatt
      421 tgttctcaat aacaacttcc ccagaccttc ctgcaaatca ggaaaaacac tttgcaaatt
      481 ttgtccttca gtatcctgta aaaaattgtt aagatcattc attactgttt ctatctcatt
      541 tccatgtgca tagtaaatgt agcctaagag tttccgtaat aagctaacta tctcatttga
      601 aacacccggg cttgttgcac ttacactatc aagaaagcta tataaatctt ccgtatactt
      661 ggtataggta tcctgtggcc agtcatagtc ttcctgatgc attattctat ataaaatctg
      721 acgcaacaat ccaatggtat caaccagaac gggaagagca tccgtcgttg ggggcaaaat
      781 ttcctttaca gccaaaacat cagcatttac cgcatcggca agcattatat taaatgtatg
      841 ctggtctaac tttgaaacag catcatacaa cgcaggatat tcatccagta gatcaaaaag
      901 tctatattcg ggctcaggat cattgcgctg tgaaaaaaat gttgaatcac cagagcaacc
      961 taacattgaa acaaatatac acagtatgat tggagcatat attttcttta tagcagttat
     1021 acatctcatg gtaaacctca tatacagaaa aagtatttaa gaaatgtttc atatatactg
     1081 ttcatatatt ttcatcttta acgtataact atcgcacaag atattaaaca accttacaac
     1141 aggctaacga catgccatcg ttaacctgtt gtatgctgta ttattttgat aaaatcaacc
     1201 ccagtgtttg gtatggatta aaatccgggc taaatatata gtcaccggca tcaccgttat
     1261 atggcgtctg ataaacaaac ctatcaaagc tgccatcact ttgtgattga tacacacctt
     1321 tttccgcttc atcaaggtta atgtacagta atgtgagtat ttgccccagg tttgaccaca
     1381 acgctgaatt gggattggta attaactcat caccaagtaa atcatatatt tgttgcaata
     1441 attcttcagc agtgtagctg gtattgaagt cctgtaaaaa ttcttccagg aacccatcat
     1501 cggcaagcat gtcattcaca aatgacagta aatcattata atttcctgca taggatgcca
     1561 gaatgcctgg caggttatca gtaagaagat tctttaaatc agcgggcaca acccaggtgc
     1621 tggttgaata atcgtattta tacagcaaca cacccagcat atggcgcaac gctgttaggt
     1681 gtttgtcttc aagttctaca cctgtaaaca ttttatcaag cagtgaaata agatccgggg
     1741 taatacaata ctgaccctgt gttgtcccgg tagtgcttaa taattcagca ataccgttaa
     1801 acatcttgtc aaaatttatc cagttcctat cagggtttga attattcaga gggcgattat
     1861 caacaaatac cgcaagaccc ttttcaccgg tttcaggtat ttcatcagta tctgatgtgc
     1921 caataaattc attcaatcca atgtccagat caacatcaag ggaagcatct ttagtaaata
     1981 tccagccagg atagctccat ccagtagtac ttatattaaa gtatgaaaag ccactggaaa
     2041 gctcagtgct ctggctacat tttatacgtg taattatctg ctctaaacca tagaaaagct
     2101 ttcgtctgct tccccattgt gtgtaatcac tgcttgtttt atcactatca acatatatgg
     2161 gatttgcaga tggtccatat tgcccaacat cctgaagcat agctaaaagc ttgcttacta
     2221 ctttcccatt agctaatgct ggtattatac catctgcagc agccgttgta ttttcagtta
     2281 aaatattggc aacagtacgc actgaatctt tgggtttaaa atctacagca gtgctaacag
     2341 gtttaggagt aaagaatcca aaat
//
LOCUS       DAORYL010000188         2340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_30619_length_2340_cov_14.195624, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000188 DAORYL010000000
VERSION     DAORYL010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30619_length_2340_cov_14.195624"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /gene="nusB"
                     /locus_tag="PLV81_15545"
     CDS             <1..294
                     /gene="nusB"
                     /locus_tag="PLV81_15545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOM11555.1"
                     /translation="DIRDFAITLIEGTIEHLDFIDDLIKRYSKNWKFERINPVDKSIL
                     RISIYALLYLDSIPHAVTIDEGIELGKIYGSENSGQFINGILDAIRVHELQTR"
     gene            298..>2340
                     /locus_tag="PLV81_15550"
     CDS             298..>2340
                     /locus_tag="PLV81_15550"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM11556.1"
                     /translation="MPPIKKHRFTIIPEEKQEGMRIFSIENEKSIKKGFTISIPKKYH
                     PLIAALFLILFVVLIVAIVTVIKKPSSFLDQKQTIAQDNTLLPGLSDQKLIPEDLSQN
                     PHILRGKTYYFKKNYTSAIDEFNEVVASDVPDSDKAIALTYIGMIYDDRGDYTKAIET
                     FLRALKYKPKDSTILKNLALAYRHQKDYPNALATTEKAIDIDENNPDLLILKGNILFD
                     MGRFPDAIKSYTKALDIAPNNPQALYNTALTFLKTGDELSAVEYFKKAATADASGTIA
                     KLAYGRLGAFYTGRRDYAMAEKYLNMAIQLDPNEPVNHYNLGIVYMQRQQYEKALTEF
                     KVAESSGANEEKLLEGLSEAYASLERYDESIAVLERLQPVQNRNVAIIARLAELYYRK
                     GDLDTAFEYYKQITVYEPVSENARVAYANMGNIRDDQHRYEEAVSFYKKALAIDPKDS
                     STLYNLGIAYKHAGKPELAVAAFNDAAQYNPDNPKPRLAIADLYYEKGYLDLALDEYI
                     KVVRLWPNLQEAQFTLGMLYFNKGRNDYALEAFNRVIAINGNNEYAKKAYVNIGIILS
                     GSKDEKQQAQAVQSLQKALLISPNEPEALLAMGTIAYNQHKYDKALEIFYQVLESSSE
                     DSITAQAYHNIGRCYYAKREYKKSLQFLTKASELDPTNEEIRLNRKVAMEAYEKELS"
BASE COUNT          807 a          476 c          451 g          606 t
ORIGIN      
        1 gatatcaggg attttgccat tacattaatt gaaggcacta tagaacattt agattttatt
       61 gatgacctga taaaacgata ttcaaaaaac tggaaatttg aacgcatcaa ccctgttgat
      121 aagtctatac tgagaatttc tatctatgcc cttttatact tagattccat accccatgca
      181 gtcactattg atgaagggat tgaactggga aaaatttatg gaagtgaaaa ctctggccag
      241 tttataaacg gaatccttga tgcaatacgg gtacatgaac tccaaacgag gtgaaacatg
      301 cctcctataa aaaaacatag gttcacaatc attccagaag aaaaacagga aggaatgcgc
      361 atcttttcaa tagaaaatga aaaatccata aaaaaaggtt ttaccatatc aataccaaaa
      421 aaatatcatc ccttaattgc agcattgttc cttattcttt ttgttgtact tattgtggcg
      481 atagtaactg tcataaaaaa accttcatca ttccttgatc aaaagcaaac cattgcacag
      541 gacaatacac tgctaccagg cttaagcgac cagaagctta tcccggagga tctatcgcaa
      601 aatccgcaca tactgcgtgg caaaacatat tattttaaaa agaattatac cagtgccatt
      661 gatgaattta atgaggtggt tgcatccgat gtacctgaca gcgataaggc tattgccctt
      721 acctatattg gcatgatcta cgatgacaga ggcgactata ctaaagccat tgaaacattc
      781 ctgcgagcat taaaatataa acccaaagac agcactattt taaaaaattt agcacttgcc
      841 tacagacacc aaaaagacta tcccaatgca ctggctacca cggaaaaagc cattgacatt
      901 gatgaaaaca atcctgattt actgatactt aaaggaaata tactctttga tatgggcagg
      961 tttcccgatg ccattaaaag ctacacaaaa gcactggaca ttgcacccaa caacccacaa
     1021 gccctctaca atacagcact aacattcctg aaaaccggtg acgaattatc agcagtggaa
     1081 tactttaaaa aggctgcaac agctgatgca tcaggaacta tagcaaaatt agcctatggc
     1141 cgtttaggtg cattttatac aggcaggcgt gactatgcaa tggctgaaaa atacctgaac
     1201 atggccatac agcttgaccc caatgagcca gttaatcact ataatttagg tatagtgtat
     1261 atgcagcgcc agcagtatga aaaggcactg actgaattca aagtagctga atcatctggt
     1321 gcaaatgaag aaaaactttt agaagggctt tcagaggctt atgcatcgct tgaacgctat
     1381 gatgaaagta ttgcggtact tgaacgcctg cagccagtgc aaaacaggaa tgttgccatc
     1441 attgcacgat tagctgaatt atattaccgc aaaggtgatt tagatactgc gtttgaatat
     1501 tacaagcaga ttaccgtgta tgagcctgtt tcggaaaatg cccgagtagc ttatgccaat
     1561 atgggcaata tccgtgatga tcagcaccgg tatgaagaag cagttagctt ttataaaaag
     1621 gcacttgcca tagatcctaa ggacagttcc accctgtata acttagggat tgcctacaaa
     1681 catgctggaa aacctgagct tgccgttgca gcatttaacg acgctgcaca gtataatccc
     1741 gacaatccca aaccacggct tgccattgct gacctgtatt atgaaaaagg gtatctggac
     1801 ttagcgctgg atgaatacat aaaagtggtg cggctgtggc ccaatttgca ggaagctcag
     1861 tttacacttg gtatgctcta tttcaataaa ggaagaaatg attatgcact ggaggcattc
     1921 aacagagtaa ttgcaataaa tggcaacaat gaatatgcaa aaaaagctta tgtaaatatt
     1981 ggcattatac tatctggttc aaaggatgaa aaacagcagg cacaggctgt acagtcactc
     2041 caaaaggcat tgcttatttc acccaatgaa cccgaagcgc ttcttgcaat gggcactatt
     2101 gcatacaacc agcataaata tgacaaggca ctggagattt tttatcaggt acttgaatct
     2161 tccagtgagg atagcatcac tgcacaggca tatcataata ttggacgttg ctactacgct
     2221 aaacgcgaat acaaaaaatc gctgcagttc ttaactaaag cttcagagct tgacccaacc
     2281 aatgaagaaa tacgtctaaa tcgtaaagta gcaatggaag cgtatgaaaa ggaactttct
//
LOCUS       DAORYL010000189         2339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_30643_length_2339_cov_11.758757, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000189 DAORYL010000000
VERSION     DAORYL010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30643_length_2339_cov_11.758757"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PLV81_15555"
     CDS             <1..612
                     /locus_tag="PLV81_15555"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOM11557.1"
                     /translation="LFYILEYNGILQTLTFKEKMLASFFQSVTARTAGFNTVDIGALR
                     EGTCLVLIFLMFIGGSPGSIAGGVKTSTFAILWLFLISRLKGLRQITVWERALAYDNV
                     ERAVTLVVVSGLFIFFATFILVVIPTLPTGEFLQSFFEITSAFGTVGLSMGITPKTVQ
                     LERIILCVVMYTGRLGPLTLISALTMRKKTINIKYAEDHIMIG"
     gene            622..1308
                     /locus_tag="PLV81_15560"
     CDS             622..1308
                     /locus_tag="PLV81_15560"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOM11558.1"
                     /translation="MKKYFVIGLGNFGFNIARALEENGCEVLGMDIDRELVQRAKDFI
                     SHAIIGDASDREVLESLAISDFDGAVISIGQDMAASILIALYLKEIGMQKIIVRAISE
                     DHGKILTLIGVDTVVFPERDMAIRLASKLALKNAMDYLPLTEEYGILEVVPPKSFINK
                     SLKELKLSTRFGCQVIGLKYYANGNYNDVTEKNAQIKIAPTADDVVTEHSVMVVIGKL
                     SDIERLQNAD"
     gene            complement(1309..2265)
                     /locus_tag="PLV81_15565"
     CDS             complement(1309..2265)
                     /locus_tag="PLV81_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOM11559.1"
                     /translation="MKVKFWGVRGSIPTTLNSVTIRDKIKQALQLATPKDIANDESIE
                     TFLDSLPTSIVGTYGGNTTTLEVRTASDDLIIIDCGTGLKHLGNELLKGDFGNGKGFA
                     TILLTHTHWDHIQGIPFFAPFYIKGNRFNIYSPYNDIKERIEYQQVFTHFPINLEYML
                     AQKEFFIIEKESEININDVKIKNKRMRHPGGSFGFRFEENGKSFVFTSDCEFNIDEID
                     KIDSYKEFFENADVCVFDTQYTFEESINKVDWGHSSASIAIDIAAMFNIKRLILFHHE
                     PDYNDNKLDAVLSNAKLYLGMNPSRRGKLTIDIAREGMEIEI"
BASE COUNT          710 a          369 c          468 g          792 t
ORIGIN      
        1 cttttttata tactggaata taatggcatt ttgcaaactt taactttcaa ggaaaagatg
       61 ttagcatcat ttttccagtc ggttactgca cgtactgccg gttttaatac cgtagatatt
      121 ggagctttgc gtgaaggtac ctgcctggta ttaatattcc ttatgtttat tggtggttcg
      181 ccaggttcta ttgctggtgg tgttaagaca agtacatttg caatattatg gttgtttctg
      241 atttcacggt taaaaggatt gcgccagatt acagtatggg aacgcgcact ggcctatgat
      301 aatgtagaaa gagccgttac attagtggta gtttcaggat tgtttatttt ctttgcaaca
      361 tttattcttg ttgtgatacc aacactgccc acgggggaat ttttacagtc tttttttgaa
      421 ataacatcag catttggtac ggtagggctt tcaatgggaa taacgccaaa gacagttcag
      481 cttgaaagga taattttgtg tgttgtcatg tatacgggaa ggttaggtcc gttgacgtta
      541 atttcagcat tgactatgcg taaaaaaacc attaatatca aatatgctga agatcatata
      601 atgattgggt aaaggttgtg tatgaaaaaa tattttgtta ttgggttggg taattttggg
      661 tttaatattg caagagcact tgaagaaaat ggctgtgaag ttcttggcat ggatattgac
      721 agggagcttg ttcagagagc aaaggatttt ataagccatg ctataatagg ggatgcctcg
      781 gacagagaag ttcttgaatc gcttgcaata agcgattttg acggtgctgt tatcagcatt
      841 gggcaggaca tggcagcaag cattcttatt gcattgtatt taaaggagat aggcatgcaa
      901 aagattattg ttagagctat ctccgaagat catggaaaga ttttaacatt gattggtgta
      961 gatactgttg tattccctga acgtgatatg gcaatacgtc ttgccagcaa attggcactg
     1021 aaaaatgcaa tggattatct tcctcttact gaagaatatg gaatccttga agttgtgccc
     1081 cccaaaagtt tcattaacaa atcacttaaa gaactgaaac ttagtacacg ttttggatgc
     1141 caggttatag gtctgaagta ttatgctaac gggaattata atgatgttac ggaaaaaaat
     1201 gctcagatta aaattgcacc cacggctgat gatgttgtaa ctgagcattc ggttatggtg
     1261 gtaataggca agttaagcga tattgaacgc ctgcaaaatg ctgactgatt atatttctat
     1321 ttccatgcct tcacgtgcga tgtcaatggt taattttcca cgtcgtgaag gattcatgcc
     1381 caaataaagc tttgcattag ataaaacagc atctaattta ttatcattgt aatctggttc
     1441 gtgatgaaac agtatcaatc gcttgatgtt gaacattgca gcaatatcaa ttgcaattga
     1501 agccgaagaa tgtccccaat ccactttatt aattgattct tcaaaggtat actgtgtatc
     1561 aaaaacgcat acatcagcat tttcaaaaaa ttctttatat gaatcaattt tatcaatttc
     1621 atcaatatta aattcacaat cactggtaaa tacaaaagac ttgccatttt cctcaaatct
     1681 gaaaccaaag gagccaccag gatgtcgcat gcgtttgttt ttaattttga catcattaat
     1741 gttaatttca ctttcttttt caattataaa gaattctttc tgagcaagca tatattcaag
     1801 attaataggg aagtgtgtaa aaacctgctg atattcaatg cgttctttga tatcattgta
     1861 tggagaatag atattaaaac gattcccttt aatataaaat ggggcaaaaa aaggtattcc
     1921 ctgtatgtgg tcccagtgtg tgtgtgtaag cagtatagta gcaaatcctt ttccattacc
     1981 aaaatcaccc tttaaaagtt cattgcctag atgttttaaa ccggtgccgc aatcaataat
     2041 gattaaatca tctgaagctg ttcgtacttc cagtgtagtt gtgttaccac catatgttcc
     2101 aacaattgat gttggcagtg aatctaaaaa agtttcaatt gattcatcat tagcaatatc
     2161 ttttggggtt gctaattgaa gtgcctgttt tattttatca cgtatagtaa cagagttaag
     2221 tgtggtgggt attgaacctc gtactcccca gaatttaacc ttcatacagg tgcctcttca
     2281 attttatctt atgtatatta tataatatcg tggtttttat gagaatcaag aataatttt
//
LOCUS       DAORYL010000190         2315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_31083_length_2315_cov_10.144248, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000190 DAORYL010000000
VERSION     DAORYL010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31083_length_2315_cov_10.144248"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2315
                     /locus_tag="PLV81_15570"
     CDS             <1..>2315
                     /locus_tag="PLV81_15570"
                     /inference="COORDINATES: protein motif:HMM:NF015497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HOM11560.1"
                     /translation="ECNNTYIDTVRIYKAEGGVFTLYADTMNSPIATIDDSTLNDNGW
                     IDIPVRRTLSKLILVIEPVTVAQIAHVELWGLQNQLSGIYTQYRFADADAVHNTCGTA
                     IDVYDATIEDNETVEGKVTVPNPWQYRRVYIQMLAGGVFRPVSVAVKVNECDWLGGLT
                     IPEEAGIGDDVREHTIEVNPQWFVPGQNSVYIQTRGGTVTVKKLRVIAVKDTGFNELQ
                     RNDKALYDGNTATYETVGNEGAIVTGDRKMRIYGALVYMHEGAVTLQAQTERGWVNIA
                     GVVAKESGWYYSACEKAIVTDTLRVVAAQDKAVTVGEVALCASPAEEQVPEIVISYPR
                     NGEYFGRTAHIEGFVHPYATQDGVAGLTVNGRELTCGIAGGFTAAISKNMVGLGQEDD
                     SKPWQLTFVAEYGRSGVQAVERVYSSSTIRKVQTTLVLDDGFMPYTQGSGGGITSPAP
                     VSYPGSDVPSGTGLTVTVAPDSAKSVYYQGVQLEIPKGAVDETVTVTIIPLGEHELAP
                     LTPGMRNVTAPAAGYRFLFNGKPHGSFKKAITISIPYDSGKLLSGTSEKQIQMYYYDD
                     GAAVWKRMEGSSAVTKKDTDYSLYARSVISSTGASGVVVAKSTHFTDFINATLTVPEH
                     PEALLFNPNSIKDIKAGDPVSGMQLIQPPQVSNTGDAGIRFPITIPKGRAGMQPQLEL
                     TYSQQGGSGPLGLGWNMRISSISIDTKWGAARYDGTDVYSLDGSQLVPVDNGGNYRTR
                     IEGGFAKIYYDKQSNCWTVTHKNGVRYIYGE"
BASE COUNT          700 a          422 c          627 g          566 t
ORIGIN      
        1 agaatgcaat aatacctata tagatactgt tcgcatatat aaagcagaag gtggagtgtt
       61 taccctgtac gctgatacca tgaattcccc cattgccacc attgatgact caaccttaaa
      121 tgataatggt tggattgata ttcccgttag gcgcaccctt agcaaactta tacttgttat
      181 agagccggta acagtagcac aaatagccca tgtagagttg tggggcttgc agaatcagct
      241 gagtggtata tatacgcagt acaggtttgc ggatgcagat gcagtgcata atacctgtgg
      301 aacagctata gatgtatatg atgcaacaat agaagacaat gaaactgtag aagggaaagt
      361 gacagttcct aacccctggc agtaccgtcg tgtatatata cagatgcttg ctggcggtgt
      421 tttcaggccg gtaagtgtgg cagtaaaggt aaatgaatgt gactggctgg ggggacttac
      481 catacctgaa gaggcgggta taggggatga tgtacgtgag cataccattg aagtaaatcc
      541 acagtggttt gttccgggtc aaaattcagt gtatatacaa acaaggggag gcacagtaac
      601 ggtaaaaaaa ctgcgcgtta ttgcagttaa ggataccggt tttaatgagc tacagcgtaa
      661 tgacaaagct ctgtatgatg gtaatactgc aacttatgaa acggttggga atgagggggc
      721 gatagtaact ggtgacagaa agatgcgtat ttatggagca cttgtatata tgcatgaggg
      781 agcagtgacg ctacaggcac aaacagaacg tggatgggtt aatatagcag gagtggtagc
      841 taaagaaagt ggctggtatt acagtgcatg tgaaaaagcg atagtgaccg atacacttcg
      901 ggttgttgca gcgcaggata aagcggtaac tgtgggagaa gttgcactat gtgcatcacc
      961 ggcagaagaa caggtgcccg agattgttat aagttatcca cgcaatggtg aatattttgg
     1021 gcgtacagca catatagaag gttttgtaca tccatatgcc actcaggatg gagtggcagg
     1081 gcttaccgta aatggcagag agcttacctg tggtattgct ggtggattta ccgctgccat
     1141 aagcaaaaat atggttggcc ttgggcagga agatgacagt aaaccctggc agttaacgtt
     1201 tgttgctgag tatggaagat caggagtgca ggctgttgaa cgagtatata gcagtagcac
     1261 aatcagaaaa gtccagacaa cactggtgct tgatgatggc tttatgccgt atacacaggg
     1321 gagtggtggt ggaataactt caccagcgcc ggtaagttac ccgggaagcg atgtgccttc
     1381 aggaactggc ttaacagtaa cagtggcacc cgatagcgcc aagagtgtat actatcaggg
     1441 agtgcaactg gaaataccaa aaggagcggt tgatgaaact gttacggtaa ccattatacc
     1501 gctgggagag catgaacttg caccacttac accgggtatg cggaatgtga cagcgcctgc
     1561 tgccggatac cgctttttgt ttaacgggaa acctcatggt agctttaaaa aagccataac
     1621 cataagtatt ccgtatgaca gtggtaagct attaagcggt accagtgaga aacagataca
     1681 aatgtactac tacgatgatg gagcggctgt atggaaacgt atggagggaa gtagcgcagt
     1741 aacaaaaaag gataccgatt attccctgta tgctcgaagt gtcataagct ctaccggtgc
     1801 atccggagtg gtggtggcaa aatcaacgca ctttacggat tttattaatg caacgttaac
     1861 agtaccggag catcccgaag cccttttatt taatcccaat agtataaaag atattaaggc
     1921 tggtgatccg gtaagtggta tgcaactaat acaaccacct caggtaagta acactggtga
     1981 tgccggaata cgatttccca ttaccatacc aaaagggcga gcggggatgc aaccgcagct
     2041 tgagctaact tacagccagc agggtggaag tggccccttg ggattaggat ggaatatgcg
     2101 catttcatca atcagcattg atactaagtg gggtgctgct cgatacgatg gtacggatgt
     2161 gtatagtctt gatggcagcc agcttgttcc agttgataat ggcggtaact atcgcacacg
     2221 cattgaaggg ggttttgcaa aaatatatta tgacaaacaa agtaattgct ggacagtgac
     2281 acacaaaaat ggggtgcggt atatatatgg tgaaa
//
LOCUS       DAORYL010000191         2282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_31694_length_2282_cov_12.298159, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000191 DAORYL010000000
VERSION     DAORYL010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31694_length_2282_cov_12.298159"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /gene="aroF"
                     /locus_tag="PLV81_15575"
     CDS             <1..878
                     /gene="aroF"
                     /locus_tag="PLV81_15575"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOM11561.1"
                     /translation="IREQPLEAISGVESVKPILKPYKLASKETHPDRTIIRVGDIEIG
                     GDKIVLVAGPCAVEGEREIIEHAKMVKQAGASMLRAGAYKPRSSPYTFQGYGALGLKY
                     LAKAKEETGLPIVTEVMDPRDVELVAQYADVLQVGTRNMQNFNLLKELGKINKPILLK
                     RGMSATINEFLMSAEYILSQGNKQVILCPRGIRTFEDITRNTLDVGAVPVLKDFSHLP
                     VVCDPSHAMGLKDYVTSAALAYVAAGADGIMVEAHPNPEEAMSDGQQTINFEQLVDLK
                     KKLASVALALGRGIY"
     gene            878..1756
                     /locus_tag="PLV81_15580"
     CDS             878..1756
                     /locus_tag="PLV81_15580"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HOM11562.1"
                     /translation="MFNKVAIIGLGLIGGSIARMLKKKHQSVHITAYGRKPENLHNAL
                     KEGFIDNVASIDAIDCNDVDLFIVATPVLSSIAIIKSLLDDPHLKSSALIIDAGSVKQ
                     SIIDSVIEHQKAHQFVGCHPMAGSEKSGYTFSTHSILTGAWVIITPHKKNNDDDIDII
                     KKFWELLECKIHITDAVTHDRMVASTSHFPHIVSTVLMESFFAINKENNPSDIQPFIG
                     KGFKDMTRLAGGSPDMWSDIVLLNNTNIIAVLEDYIQSLNKVKHRIQQASLHNDRDVL
                     CRYFEEVQKKWIKYHA"
     gene            1749..>2282
                     /gene="cmk"
                     /locus_tag="PLV81_15585"
     CDS             1749..>2282
                     /gene="cmk"
                     /locus_tag="PLV81_15585"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOM11563.1"
                     /translation="MRKNIVVAVDGPAGSGKSSVSKEVAKRAGLQYIDSGALYRAITL
                     YVLEHCGEQFTQDDVRKMLDKIQLKQVFNNDGSCTTYCNGVDVSENIRNETVATHIGK
                     ISDIVAVREYVNMLMRMWAKDHSIIVDGRDIGTVVFPDADIKIYLDATVEERALRRAR
                     EYAQKGKNIDVNELKVHI"
BASE COUNT          715 a          391 c          541 g          635 t
ORIGIN      
        1 tcatccgtga acagccactt gaagcaatat caggtgttga atcggtcaag ccaatcttaa
       61 aaccgtacaa gttagcaagt aaagaaacac atcccgacag aaccattatt cgtgtgggtg
      121 atattgagat tggcggggat aaaattgtgc tggtggccgg tccctgtgct gttgaaggtg
      181 aaagggaaat tattgagcat gcaaagatgg ttaagcaggc tggtgcatcc atgttgcggg
      241 caggcgcata caagcctcga tcatcgccgt acacgtttca agggtatggt gcactgggtt
      301 taaagtattt agcaaaggca aaagaggaaa cagggttacc gatagttacc gaggtgatgg
      361 acccccgtga tgtagaactt gttgcgcagt atgctgatgt attgcaggtt ggtacccgca
      421 atatgcagaa ttttaacctt ttaaaggaac tgggaaaaat aaataagcca attttgttaa
      481 agcgagggat gagtgcaacc ataaatgaat tcttgatgtc agcagagtat atactttcac
      541 aggggaacaa acaggtaata ctatgcccac gtggaatacg gacatttgag gatattaccc
      601 gcaatactct tgatgttggt gcagtaccgg tgttaaaaga tttttcacat ttacctgttg
      661 tgtgtgatcc aagccatgca atgggactaa aagattatgt gacttcagca gcacttgctt
      721 atgtggctgc aggcgctgat ggcatcatgg ttgaagcaca ccccaatcct gaagaagcaa
      781 tgtctgatgg tcagcagaca ataaattttg aacagttagt tgatcttaaa aagaaactgg
      841 ccagcgtggc actggcactg ggcaggggaa tttattaatg ttcaataagg ttgctattat
      901 aggtttgggg cttattggcg gatcaattgc acgcatgctt aaaaaaaagc atcaatcggt
      961 gcatattacc gcgtatggcc gtaaaccaga aaatctgcac aatgctctta aagaaggatt
     1021 tattgataat gttgcttcta ttgatgcaat tgattgcaat gatgttgatc tttttattgt
     1081 agcaacaccg gtgctatcgt ctatcgcaat tattaaatcc ttgctggatg atccgcatct
     1141 taaatccagt gcattaatca tagatgctgg tagtgtaaag caatctatca tagattcggt
     1201 aatagaacac cagaaggcac atcagtttgt tggatgccat ccaatggcag gttctgaaaa
     1261 gtccggatat accttcagta cccactcaat ccttaccggt gcatgggtaa ttattacacc
     1321 gcacaaaaaa aacaacgatg atgatataga tatcattaaa aaattctggg aattgttgga
     1381 atgcaaaatt catattaccg atgctgtaac acacgaccga atggttgcaa gtaccagcca
     1441 tttccctcat atagtatcaa cagtattaat ggaaagtttt tttgctatta ataaagaaaa
     1501 taacccatca gacatccagc catttattgg caaaggattc aaagatatga cgcggcttgc
     1561 tggtggtagt cctgacatgt ggtctgatat tgtgttgtta aataatacca atatcattgc
     1621 agtgcttgaa gattatatac aatcattgaa taaggtgaaa catcgtatac agcaagcatc
     1681 gcttcataat gaccgggatg tgctgtgccg gtattttgaa gaagttcaga agaaatggat
     1741 taagtatcat gcgtaagaat attgttgtgg ctgttgatgg acctgcaggt tccgggaaaa
     1801 gttctgtatc aaaagaggtg gctaaacgag caggtttgca atatatcgat tcgggagcat
     1861 tgtacagggc aataactctg tatgtgctgg agcattgtgg tgaacagttt acacaggatg
     1921 atgtgcgcaa gatgcttgat aaaatccagc ttaaacaggt ttttaataat gatggaagct
     1981 gcacaacgta ttgtaatggt gttgatgtta gtgaaaacat acgcaatgaa accgtagcca
     2041 cacatattgg caaaatttca gatatcgttg cagtacgaga atatgtcaat atgctcatgc
     2101 gcatgtgggc taaagaccat tcaatcattg tggatggaag ggatattggt actgttgtgt
     2161 ttcctgatgc agatataaag atatatttag atgcaacagt tgaagaacgt gcattgcggc
     2221 gtgccagaga atatgcgcaa aaaggaaaaa atattgacgt aaatgagtta aaggtgcaca
     2281 tt
//
LOCUS       DAORYL010000192         2282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_31695_length_2282_cov_11.713965, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000192 DAORYL010000000
VERSION     DAORYL010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31695_length_2282_cov_11.713965"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /gene="cmk"
                     /locus_tag="PLV81_15590"
     CDS             complement(<1..534)
                     /gene="cmk"
                     /locus_tag="PLV81_15590"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOM11564.1"
                     /translation="MRKNIVVAVDGPAGSGKSSVSKEVAKRAGLQYIDSGALYRAITL
                     YVLEHCGEQFTQDDVRKMLDKIQLKQVFNNDGSCTTYCNGVDVSENIRNEIVATHIGK
                     ISDIVAVRDYVNTLMRMWAKDHSIIVDGRDIGTIVFPDADIKIYLDATVEERALRRAK
                     EYAQKGKNIDVNELKVHI"
     gene            complement(527..1405)
                     /locus_tag="PLV81_15595"
     CDS             complement(527..1405)
                     /locus_tag="PLV81_15595"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HOM11565.1"
                     /translation="MFNKVAIIGLGLIGGSIARMLKKKHQSVHITAYGRKPENLHNAL
                     KEGFIDNVASIDAIDCNDVDLFIVATPVLSSIAIIKSLLDDPHLKSSALIIDAGSVKQ
                     SIIDSVIEHQKAHQFVGCHPMAGSEKSGYTFSTHSILTGAWVIITPHKKNNDDDIDII
                     KKFWELLECKIHITDAVTHDRMVASTSHFPHIVSTVLMESFFAINKENNPSDIQPFIG
                     KGFKDMTRLAGGSPDMWSDIVLLNNTNIIAVLEDYIQSLNKVKHRIQQASLHNDRDVL
                     CRYFEEVQKKWIKYHA"
     gene            complement(1405..>2282)
                     /gene="aroF"
                     /locus_tag="PLV81_15600"
     CDS             complement(1405..>2282)
                     /gene="aroF"
                     /locus_tag="PLV81_15600"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOM11566.1"
                     /translation="IREQPLEAISGVESVKPILKPYKLASKETHPDRTIIRVGDIDIG
                     GDKIVLVAGPCAVEGEKEIIEHAKMVKQAGASMLRAGAYKPRSSPYTFQGYGALGLKY
                     LAKAKEETGLPIITEVMDPRDVELVAQYADVLQVGTRNMQNFNLLKELGKINKPILLK
                     RGMSATINEFLMSAEYILSQGNKQVILCPRGIRTFEDITRNTLDVGAVPVLKDFSHLP
                     VVCDPSHAMGLKDYVTSAALAYVAAGADGIMVEAHPNPEEAMSDGQQTINFEQLVDLK
                     KKLASVALALGRGIY"
BASE COUNT          632 a          536 c          390 g          724 t
ORIGIN      
        1 aatgtgcacc tttaactcat ttacgtcaat attttttcct ttttgcgcat attctttagc
       61 acgccgcaat gcacgttctt caactgttgc atctaaatat atcttgatat ctgcatcagg
      121 aaacacaata gtgccaatat ccctcccatc cacaatgatt gaatggtctt tggcccacat
      181 acgcatgagt gtattgacat aatcccgcac cgcaactata tccgaaattt taccaatatg
      241 tgtggctaca atttcattgc gtatgttttc actaacatcg acaccattac aatatgttgt
      301 gcagcttcca tcattattaa aaacctgttt aagctggatt ttatcaagca tcttgcgcac
      361 atcatcctgt gtaaactgtt caccacaatg ctccagcaca tacagagtta ttgccctgta
      421 caatgctccc gaatcgatat attgcaaacc tgctcgttta gccacctctt ttgatacaga
      481 acttttcccg gaacctgcag gtccatcaac agccacaaca atattcttac gcatgatact
      541 taatccattt cttctgaact tcttcaaaat accggcacag cacatcccgg tcattatgaa
      601 gcgatgcttg ctgtatacga tgtttcacct tattcaatga ttgtatataa tcttcaagca
      661 ctgcaatgat attggtatta tttaacaaca caatatcaga ccacatgtca ggactaccac
      721 cagcaagccg cgtcatatct ttgaatcctt tgccaataaa tggctggatg tctgatgggt
      781 tattttcttt attaatagca aaaaaacttt ccattaatac tgttgatact atatgaggga
      841 aatggctggt acttgcaacc attcggtcgt gtgttacagc atcggtaata tgaattttgc
      901 attccaacaa ttcccagaat tttttaatga tatctatatc atcatcgttg ttttttttgt
      961 gcggtgtaat aattacccat gcaccggtaa ggattgagtg ggtactgaag gtatatccgg
     1021 acttttcaga acctgccatt ggatggcatc caacaaactg atgtgccttc tggtgttcta
     1081 ttaccgaatc tatgatagat tgctttacac taccagcatc tatgattaat gcactggatt
     1141 taagatgcgg atcatccagc aaggatttaa taattgcgat agacgatagc accggtgttg
     1201 ctacaataaa aagatcaaca tcattgcaat caattgcatc aatagaagca acattatcaa
     1261 taaatccttc tttaagagca ttgtgcagat tttctggttt acggccatac gcggtaatat
     1321 gcaccgattg atgctttttt ttaagcatgc gtgcaattga tccgccaata agccccaaac
     1381 ctataatagc aaccttattg aacattaata aattcccctg cccagtgcca gtgccacgct
     1441 ggccagtttc tttttaagat caactaactg ttcaaaattt attgtctgct gaccatcaga
     1501 cattgcttct tcaggattgg ggtgtgcttc aaccatgatg ccatcagcgc ctgcagccac
     1561 ataagcaagt gctgctgaag tcacataatc ttttagtccc attgcatggc ttggatcaca
     1621 cacaacaggt aaatgtgaaa aatcttttaa caccggtact gcaccaacat caagagtatt
     1681 gcgggtaata tcctcaaatg tccgtattcc acgtgggcat agtattacct gtttgttccc
     1741 ctgtgaaagt atatactctg ctgacatcaa gaattcattt atggttgcac tcatccctcg
     1801 ctttaacaaa attggcttat ttatttttcc cagctccttt aaaaggttaa aattttgcat
     1861 attgcgggta ccaacctgca atacatcagc atactgggca acaagttcta catcacgggg
     1921 atccattacc tcggtaatta ttggtaaccc tgtttcctct tttgcctttg ctaaatactt
     1981 taaccctaac gccccatacc cttgaaacgt gtacggcgat gaccgtggct tgtatgctcc
     2041 cgcccttagc atggatgcgc cagcctgctt taccattttt gcatgctcta taatttcctt
     2101 ttcaccttca acagcacagg gaccggccac cagtacgatc ttatcaccac ctatatcaat
     2161 atcacccaca cggataatgg ttctgtcggg atgagtttct ttgcttgcta acttgtatgg
     2221 ttttaatatt ggcttgaccg attcaacacc tgatattgct tcaagtggct gttcacggat
     2281 ga
//
LOCUS       DAORYL010000193         2278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_31777_length_2278_cov_9.836257, whole genome shotgun sequence.
ACCESSION   DAORYL010000193 DAORYL010000000
VERSION     DAORYL010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31777_length_2278_cov_9.836257"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PLV81_15605"
     CDS             <1..375
                     /locus_tag="PLV81_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11567.1"
                     /translation="FTNDGDLAYKLTHPRFKVPKTYQVTLDRPLRDDHKMAIEHGIEL
                     YGTQTLPATIVPINDEWTIVTITITEGKKRQVRMMFSMLGYEVISLIRIKYGPITLGN
                     LPVGKYRVLSKQEIEKLKSAVN"
     gene            458..1009
                     /locus_tag="PLV81_15610"
     CDS             458..1009
                     /locus_tag="PLV81_15610"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOM11568.1"
                     /translation="MQNFDFHSNNKNFYTDYNPQDYREYTIPQNYATPRSFSQKKAHR
                     MLTIIIALIIMSFTAGLVVGIKFAGGTNKPIIDPQTKVALTNAAQKAKKLVEPKQQPQ
                     FPKSEYPYAIKVGNAYTRDEATALAQILSKTGQRVIIAKNKDRYNVYAGPYTSLQDAK
                     KSLKIIMEQSKNGNFSNAIILKR"
     gene            1019..1492
                     /locus_tag="PLV81_15615"
     CDS             1019..1492
                     /locus_tag="PLV81_15615"
                     /inference="COORDINATES: protein motif:HMM:NF005840.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOM11569.1"
                     /translation="MKITIRKATSDDAEQILALLSPYVKKGILLPRSKEEIEKTSDSF
                     TVAIAQKKIIGVVSQYKYNANLFEIRSLAVDSRYHHKGIGKQLVEHAVNDILQQHKDA
                     KIFCLTYNPSFFKKVGFMAVAKETLPDKIWKDCQYCQHRDNCTETAMVYSLRSRG"
     gene            complement(1481..>2278)
                     /locus_tag="PLV81_15620"
     CDS             complement(1481..>2278)
                     /locus_tag="PLV81_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11570.1"
                     /translation="YAQMPDWAMMKDKEGNTYYFDKSGKIYTDDVESAYKPVSRDGLE
                     YYTAKVKSLWKAGHRTQSLVIAKSILALKESSMNVVKSKKDINAFMRDMQMKHGKRFV
                     DINTDAWLYLTKEDTTMYVFNDKVPYIVALQGNAEIIAAKSVDSLQYSSTVMRAGIRF
                     SETSGYDAIMTIVAESFAGTIESIRVLEQHWRTVAFDTFDRELLKYSDSMALYSFVTR
                     DNVELKGMELLLAKENRGYCVRIFTTKERYDLYKETMLTIINSFSTP"
BASE COUNT          762 a          469 c          392 g          655 t
ORIGIN      
        1 tttaccaatg atggggattt agcctacaaa ctaacccacc cacggttcaa ggtgccaaaa
       61 acgtatcagg tcacgcttga tcgaccatta cgtgatgatc ataaaatggc aatagaacac
      121 ggtattgaac tgtatggcac acaaacactt cccgcaacaa ttgttccaat aaatgatgaa
      181 tggacgatag ttaccataac cataaccgaa ggcaaaaaac gacaggtgcg tatgatgttt
      241 tcaatgcttg gctatgaggt aataagtctt atccgtatta agtacgggcc cattacattg
      301 ggaaatctgc ctgtgggtaa atacagagtt ctttcaaaac aggaaataga aaaattaaaa
      361 tctgctgtta attaattcct taatacacca ttcaattaat gacgatattc tttgtactac
      421 acattataaa attttatcac aaaaggtctg atagaatatg cagaattttg attttcactc
      481 aaataataaa aacttctata ctgattacaa tccacaggat taccgcgaat atacaatacc
      541 gcaaaactat gcaacaccca gatctttttc acagaaaaaa gcacatagaa tgctgacaat
      601 tattattgct ttaataataa tgtcttttac tgcagggctt gttgttggca ttaaatttgc
      661 aggaggcaca aataaaccga ttattgatcc ccagacaaaa gtagcattga ccaatgctgc
      721 ccagaaagca aaaaaactgg ttgaacccaa acagcagcca caatttccaa aatcagaata
      781 cccgtatgcc atcaaggttg gcaatgctta taccagggat gaagccaccg cattagctca
      841 aatattaagt aaaacaggac aacgcgttat cattgcaaaa aacaaagatc ggtataacgt
      901 atatgcaggc ccctacacct cactgcagga tgcaaaaaaa tcattgaaaa taatcatgga
      961 acaatctaaa aatggcaact tcagtaatgc tattattcta aaacgctgat tttttataat
     1021 gaagattact atccgaaaag ctaccagtga tgatgcagaa caaattcttg ccttgctttc
     1081 cccgtatgta aaaaagggca tactgcttcc acgaagtaaa gaggaaattg aaaaaaccag
     1141 cgactcattt acagttgcta tcgcccaaaa aaaaattatt ggtgttgttt cacagtataa
     1201 atataatgct aacctttttg agataaggtc acttgcagtt gacagcaggt atcaccataa
     1261 gggtattggg aaacaacttg tagaacatgc agtaaatgat atattgcagc aacacaagga
     1321 tgcaaaaatt ttttgcctta cctataatcc ttctttcttt aaaaaagtag gatttatggc
     1381 agttgccaaa gaaacactcc ccgacaaaat atggaaggat tgccagtact gtcagcacag
     1441 ggataattgt actgaaacgg caatggtgta ttcgctccga tcaaggggtt gaaaagctat
     1501 tgataattgt aagcatggtt tctttgtaaa gatcataccg ctcttttgta gtaaaaatcc
     1561 tcacacaata tcccctattt tcttttgcaa gcaaaagttc catacctttg agttctacat
     1621 tgtcccgtgt aacaaatgaa tacagcgcca tgctgtcact gtatttcagt aattcacgat
     1681 caaatgtgtc aaatgcaaca gtacgccagt gctgctccaa tacacgtatg gattcaatag
     1741 taccagcaaa actttcggct actatcgtca tgatggcatc atatccacta gtttcagaga
     1801 accgtatgcc tgctctcatc acagtggaag aatattgcaa cgaatcgacc gattttgcag
     1861 caatgatttc tgcatttccc tgtaatgcca ctatatacgg aaccttgtca ttgaatacat
     1921 acattgtagt gtcttccttt gttaaatata accatgcatc tgtgttaatg tcaacaaaac
     1981 gcttcccatg cttcatctgc atatcacgca taaatgcatt tatgtctttt tttgacttta
     2041 ccacattcat ggatgattct ttaagcgcta agattgattt tgcaataaca agcgattgtg
     2101 tgcgatgccc agctttccac agggatttta cttttgcggt atagtactcc agcccatccc
     2161 gtgaaacagg tttgtatgct gattccacat catcagtata aatcttcccg cttttatcaa
     2221 aataataggt attaccctct ttatctttca tcatggccca gtcaggcatc tgggcgta
//
LOCUS       DAORYL010000194         2267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_31999_length_2267_cov_13.639241, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000194 DAORYL010000000
VERSION     DAORYL010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31999_length_2267_cov_13.639241"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            356..799
                     /locus_tag="PLV81_15625"
     CDS             356..799
                     /locus_tag="PLV81_15625"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOM11571.1"
                     /translation="MPHKFHPEHAKYLDSIVRKVLFPPLKILNTIGIKSNDVFLDIGA
                     GTGFFAIPASHMLTYGKVIATDTQEEMLQLLSHMIKQQNINNIEIIHSSENGTSLPEE
                     SVTFAFMSFVLHEVQNKPEMLLNIHRALKKGCRLAIIEFSKYAIF"
     gene            948..1193
                     /locus_tag="PLV81_15630"
     CDS             948..1193
                     /locus_tag="PLV81_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11572.1"
                     /translation="MTVSYHAKMILQAFKDKYGNDIKAGVPMPVDILNDIPQSERHTV
                     LDELINKLEFIEMIETKFPGKLFLTHKGYHYIQNIKE"
     gene            1206..1490
                     /locus_tag="PLV81_15635"
     CDS             1206..1490
                     /locus_tag="PLV81_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11573.1"
                     /translation="MDALLSMLVDLSRTFLQDVSDIQELSHLNNKLQTTLHDIVADPT
                     QKSTLNALLHQYYSEIMTLYSRYPQSTFLDTLMHRIESLLQVANTIDGKM"
     gene            1477..2115
                     /locus_tag="PLV81_15640"
     CDS             1477..2115
                     /locus_tag="PLV81_15640"
                     /inference="COORDINATES: protein motif:HMM:NF002305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosylase/DNA lyase"
                     /protein_id="HOM11574.1"
                     /translation="MEKCDIQTLHQLCYQLEFQIHLRINSFKRLWTEKDGQYLLLELV
                     FCLLTPQSNAYLCWEKALELFNSGKLYNGTIEEISTIIHPVRFKNNKAKFIKQAVNVF
                     GDSSLNEFLQGNDIYSIRDKLVKTVSGLGYKEASHFLRNIGIGLELAILDRHILRCLK
                     DFHVINTIPDTLTSQRYKDIEYAMQDFCKSINIPISHLDFIFWYLKKKSLFK"
BASE COUNT          771 a          404 c          379 g          713 t
ORIGIN      
        1 acttagtgta tcggcatatt ataaaaatcc tttataataa tttttgcgac ataatgctat
       61 atttttaaca cgtggtataa gttaatggat atatctaaaa aattattgag ttattattgt
      121 ttcataatta atatggactt agaatagttg tatcattttg aaatgtttcc tgaaatatca
      181 gcaaagtaac ctgcaattct gatataaact cccaaaatgg cgacatgagt tataatatta
      241 gccatcatca aagcactgat tgtaaataag gaagagttag cattcaggta tatcaaaaaa
      301 ttcattttaa tgttttaaaa ttacacttat aaaaatctat tctgaggaat gtgatatgcc
      361 acataaattc catcctgagc acgcaaaata tcttgacagt atagtaagaa aggtattgtt
      421 cccaccattg aagatattga acacaatagg catcaaaagc aatgatgtct ttcttgatat
      481 tggtgccggg accgggtttt ttgcaattcc tgcatctcat atgcttactt atggcaaagt
      541 aattgctact gacacacagg aggaaatgct acagttacta tcgcacatga taaagcaaca
      601 aaacattaat aatattgaaa taatacattc cagcgaaaac ggcacatcat tgcctgaaga
      661 gtcggtaact tttgcattta tgagttttgt gttacatgaa gttcaaaaca aaccggaaat
      721 gcttttgaat attcacaggg cattgaaaaa aggctgcagg ttagcaatca tagaattcag
      781 taaatacgct attttttgac caccaaaatc cgagcgcatc agtcctgagt atgtaaaaaa
      841 attgcttgaa gaaacaggat ttacccggat tactgttaaa aagctaacaa tgtttacgta
      901 tgtagcatta gcttttaaac aataatgaac tttcactgga gcaccctatg acagtaagct
      961 accacgcaaa gatgatcctg caggctttta aggataaata tggcaatgac attaaagctg
     1021 gtgtgcccat gccagttgat atacttaatg acatccccca atccgaacgc catacggttc
     1081 ttgatgagct tattaacaaa ttagaattca ttgaaatgat tgaaacaaaa tttccaggca
     1141 agcttttttt aacacacaaa ggctatcatt atattcagaa cataaaagaa tagtctggta
     1201 cagctatgga tgctcttctg tcaatgcttg ttgacctttc acggactttt ttgcaggatg
     1261 tgagcgatat ccaggaacta tcgcacctta ataataaact gcaaactaca ctacatgaca
     1321 ttgttgctga tccaacccag aaaagcaccc ttaacgcttt actgcaccaa tattacagcg
     1381 aaataatgac actgtattct cgataccccc agagtacgtt tcttgatacc ctgatgcaca
     1441 ggattgaaag cttactacag gtagctaaca ccattgatgg aaaaatgtga cattcagacc
     1501 ctgcatcaac tttgctatca actggaattt cagattcatc tacgaattaa ttcattcaag
     1561 cggctgtgga ccgaaaaaga tggccaatat cttttgcttg aactggtatt ctgcctgctt
     1621 accccacaga gcaatgctta tttgtgctgg gaaaaagcac tggagctttt caattcaggt
     1681 aaactataca atggaactat tgaggaaatc agcacaataa ttcatccagt gaggtttaaa
     1741 aataacaaag ccaagtttat taaacaagct gttaatgtat ttggcgatag ttccttgaat
     1801 gaatttttgc aggggaatga catctactca ataagagata agctggttaa aaccgtttct
     1861 ggattgggat acaaagaagc cagccatttt ttgcgtaaca ttggcattgg gctggaactt
     1921 gcaattcttg acagacatat cttacggtgc cttaaagatt ttcatgttat taatacaatt
     1981 cccgatactc tgacctcaca gcgctataaa gatattgaat atgcaatgca ggatttttgt
     2041 aaaagcatta acatacccat atcacattta gattttattt tctggtattt aaagaagaaa
     2101 tcgttattca aataataaac atatatcaaa aaaacttatt tttgtaattc ttcacgcaat
     2161 atgccattca gttcatcaag tatttcttta aatgcagcaa gacgttcttt ttttatttta
     2221 ctttctataa aattattaat ttctttgata actttttgtg ctttctg
//
LOCUS       DAORYL010000195         2264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_32058_length_2264_cov_15.039837, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000195 DAORYL010000000
VERSION     DAORYL010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32058_length_2264_cov_15.039837"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1607)
                     /locus_tag="PLV81_15645"
     CDS             complement(<1..1607)
                     /locus_tag="PLV81_15645"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOM11575.1"
                     /translation="MASKYKGYMGKIADINLSTGSVSEYPLSDEDREKYLGGRFIATK
                     ILWDELSPGADALSPENILIVMTSPLTGTGAPSSSRYDISAKSPLTGLIGHSNSGGNF
                     GMHLKRAGFDGVVVRGKASKPVYIEFDNGSIHIRDAGNIWGMNTQDAQKAMGQGGTMA
                     IGPAGENQVLFASVVSQERSHGRCGMGAVMGSKNLKGMVARGSEKVPLHNSEEFKKHV
                     KQWITMLQKHPATGEFAPRYGTAGFLTALSMNNALPTKNFSKGSFDRAWNIGGERLAE
                     EFLVKNSGCVSCPIRCGRVVEIDGKEVKGPEYEILSLLGSNMLIDDLDAIIKWNYQLD
                     LLGLDAITTGTLMGFAAELNERGVWKCGIEFGKKDNISQIIEDIAYKRGIGPELSKGV
                     RYLAKKFNAEDYAPHVKGLELAAYEPRASVGHGLGYATASRGACHLDGGYMIYFEVTG
                     PVTLNPHHYRSKPSWTILDQNLLAAVSAGGNCLFTSWTFVPNIAYKVPGRKWLFSLLQ
                     FILTHTWILIDLTVKMPKWLMRFHVPLL"
     gene            complement(1597..2076)
                     /locus_tag="PLV81_15650"
     CDS             complement(1597..2076)
                     /locus_tag="PLV81_15650"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOM11576.1"
                     /translation="MNYRKQIKRTKKDINALMRDVLRAVYHFERFKEKYFGITYELYY
                     AMTLIDGSKKKTISDLAGSMGIPLHKATRIVQRLCSKSFVKRTTSARDKRVVYVKLTH
                     EGKKCLTTIEDFCFATVMNNLAHVSADEFTMFVQVASKIPEILALPQLNTHEVKYGK"
BASE COUNT          628 a          556 c          415 g          665 t
ORIGIN      
        1 agtagcggca catgaaagcg cataagccat ttgggcattt taacagtaag gtctatcagt
       61 atccaggtat gtgtaagaat aaactgtaac agggaaaaaa gccatttcct accgggaacc
      121 ttataggcaa tgtttggtac aaatgtccat gacgtaaaca gacaattgcc acctgcactc
      181 accgcagcca gtaaattctg atcaagtatg gtccatgaag gttttgagcg atagtgatgg
      241 ggattgagtg tcacaggccc ggtaacctca aagtatatca tgtacccacc atctaaatgg
      301 caggcacccc gcgatgctgt tgcatacccc aacccatgcc caaccgaagc acgtggctca
      361 taggcagcca actccagccc tttgacatgc ggagcataat cctcagcatt gaattttttt
      421 gctaggtacc tgactccttt agaaagctcc ggacctatac cacgcttgta ggcaatatct
      481 tcaattatct gcgatatatt gtcctttttt ccaaattcaa ttccacactt ccatacacca
      541 cgctcattta gttctgccgc aaatcccatt aatgtcccgg tggtaatggc atcaagtccc
      601 aaaagatcaa gctgatagtt ccatttaatg atggcatcta agtcatcaat aagcatgtta
      661 gaaccaagca gcgacaaaat ttcatactca ggtcctttta cttccttgcc atcaatttca
      721 acaaccctgc cacagcggat tggacatgat acgcagcctg aattttttac taaaaactct
      781 tcggctaacc gttctccacc aatattccat gcacgatcaa agctaccttt tgaaaagttt
      841 tttgtaggca atgcattgtt catagaaagc gccgttaaaa agcctgccgt accgtaccgt
      901 ggtgcaaatt caccggttgc aggatgtttc tgtaacatgg taatccactg cttgacatgt
      961 ttcttgaact cctcactgtt gtgaagagga accttctctg agccgcgtgc aaccatgcct
     1021 tttagatttt tagagcccat aacagcaccc ataccgcacc tgccatggct gcgttcctgc
     1081 gataccactg atgcaaatag cacctgattt tcacctgctg gaccaatagc catggtacca
     1141 ccttgcccca ttgccttttg agcatcctgt gtattcatcc cccagatatt gccagcatcc
     1201 ctgatgtgta tactgccatt atcaaattct atatataccg gttttgaagc tttcccacgc
     1261 accaccacac catcaaaccc tgcacgcttc aaatgcatgc caaagttgcc accgctgttg
     1321 gaatgcccaa tgaggccggt aagtggactt ttggccgata tatcatatct gctgcttgat
     1381 ggtgcaccag tacccgtcag tggcgaagtc atgacaatca agatattttc cggcgacagc
     1441 gcatcagcac cggggctaag ctcatcccac aaaatctttg tagcaataaa tcgcccacct
     1501 aaatactttt cccggtcttc atcagaaagc ggatactctg aaacacttcc tgttgaaaga
     1561 ttaatatcag caatttttcc catatatcct ttgtatttac ttgccatact tcacctcatg
     1621 tgtatttaat tgtggtaatg ccaaaatttc aggaatttta gatgccacct ggacaaacat
     1681 tgtaaactca tccgccgata catgagcaag gttattcatg actgtagcaa aacaaaagtc
     1741 ttcaattgtg gtaaggcact ttttgccctc atgggttagc tttacataca caacccgttt
     1801 atcccgtgca gatgtggtac gtttaacaaa actctttgaa cacaaccgct gcacaatacg
     1861 agttgcctta tggagcggaa ttcccatgct gccggcaagg tcacttatag tctttttctt
     1921 gctaccgtca atcaatgtca ttgcatagta taactcatag gtaatgccaa aatatttttc
     1981 tttgaagcgt tcaaaatgat atactgcgcg cagcacatca cgcatcagtg catttatatc
     2041 tttttttgtt ctttttattt gtttgcgata gttcatagta taaacctttt gccgcaatca
     2101 taaaaaaata cttgcagatt gcaagtattt ttttatgact attgcaactg gtattggctc
     2161 atggtctttg catacaatgt atcataatac caaaattaca tgcatagatt gttatatgct
     2221 ttttttaact cttgcagcga gtataccctt tttgaattgg ggag
//
LOCUS       DAORYL010000196         2244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_32462_length_2244_cov_13.827318, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000196 DAORYL010000000
VERSION     DAORYL010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32462_length_2244_cov_13.827318"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            410..>2244
                     /locus_tag="PLV81_15655"
     CDS             410..>2244
                     /locus_tag="PLV81_15655"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOM11577.1"
                     /translation="MNLLQKLNEEQYKAVTHIDGPLLILAGAGSGKTRVITYRIAYLI
                     KNALVPPFAIVAVTFTNKAAEEMQNRIKALIGPIGESVMIKTFHSLAVYILRRHGEAI
                     GIPSNFTIYDNHDQESVIKDILRDMHIDPKTVKPSMIAEKISRIKESSHFIEGGDITP
                     LLPKFHSFNFPEIFTQYHEKLKTANALDFSDLLVRTVQLLRNKEVLHKLQSRWKYFMI
                     DEYQDTNIAQYLIAKYLSSETKNICVVGDDDQSIYSWRGADIRNILNFEKDYPNATVI
                     TLHRNYRSTEPILKAAWHVIKNNYKRKDKKLTSHRGDGEPIIVCTANNEYGEAEYVIN
                     TIVTLKHKEGYTNRDFAIFYRTNAQSRIFEEHLRKENIPYVVIGSVKFYDRKEIKDII
                     AYMRFIANQHDTVSLLRIINTPARGIGASTIEKIRDAAYVNGVSEWDIISNSETYSYK
                     LSPALTAFKDTMQKLIDKAKEIPQHYKLSDICISILHDTQYKKSLEDENSIESQGRLE
                     NIDAFVNSIFEYEHLHPQATLNEFLQDISLLTPADETNGTLQDAITLMTVHNAKGLEF
                     PVVFLTGMEEGLFPHANSSDTEEGIEEERRLCYVGMTRAKDRLFLT"
BASE COUNT          765 a          415 c          393 g          671 t
ORIGIN      
        1 tgtattaaga tttctcctca cttcgttcgt cgaaatgaca tggtgaaaat gtttgccgaa
       61 atgatagtgt ataaccgttc attgaaatga cagtgtgaaa atgttcgttg aaataacatt
      121 ggaaaatcgt tcatcaaaat tacaatacac tttgctataa tatatcttca tatatacttc
      181 tgcctatata aattcaagta gctatgcaat aaaaataatt ttctttcaca tatatcaagt
      241 aaaaattata tttttactat ttttatcttg aacaattcgt agtattatta attatgtcat
      301 tttagtacag gtatcaaata atgtgctggg gattcacaaa tttattgcat ttttttgcag
      361 taatataaat gaaacaataa actacctcta aattatctaa aaatacatca tgaatttact
      421 acaaaaactt aacgaggaac aatataaggc tgtaacccat attgatgggc cactgttaat
      481 actggctggt gctggttcgg gtaaaacaag ggttattacc tataggattg cttatcttat
      541 caaaaatgct ttagttccac cgtttgccat tgttgcggta acatttacca ataaggcagc
      601 cgaagagatg caaaaccgca taaaagctct cattggcccc attggtgaaa gcgtcatgat
      661 aaaaacattt cattcgcttg cagtgtatat actgcgtcgg catggtgagg ctattggcat
      721 accttcaaat tttaccatct atgataatca tgatcaggaa tcagttatta aagatatcct
      781 gcgcgatatg cacattgatc ccaaaacagt aaaaccatca atgattgccg aaaaaatttc
      841 acgtataaaa gaaagttcac atttcatcga aggcggtgat attacaccgc ttctaccaaa
      901 atttcattca tttaattttc ctgaaatttt tacacagtac catgaaaaac ttaaaaccgc
      961 taatgccctt gatttcagcg atcttcttgt gcgtacagta cagcttttgc gcaataaaga
     1021 agtattgcat aagctgcagt cgcggtggaa atactttatg attgatgaat atcaggatac
     1081 caatattgct cagtacttaa tagcaaaata tctatcatca gagactaaaa atatatgtgt
     1141 tgttggtgat gatgatcagt caatctattc atggcgtggt gctgatatcc gcaatatatt
     1201 aaactttgaa aaagattatc ccaatgcaac agtgattacc ttacacagga actatcgctc
     1261 aactgaacca atccttaaag cagcatggca cgttattaaa aataattata aacgaaaaga
     1321 caaaaagctc acatcacatc gcggtgatgg tgagccaatc attgtgtgta cagccaacaa
     1381 tgaatacggc gaagcagaat atgttatcaa taccattgtt acccttaaac ataaagaagg
     1441 ttataccaac agggactttg caatatttta ccgcaccaat gctcaatcgc gcatctttga
     1501 agaacacctg cgtaaagaaa atattcccta cgtggttatt ggtagtgtta aattctatga
     1561 tcgtaaagag ataaaagaca tcattgcgta catgcgtttt attgcaaacc agcatgatac
     1621 tgtttcactt ctgaggatta tcaatactcc tgcacgcggc attggcgcgt caactataga
     1681 aaaaatccgt gatgctgcat atgttaacgg agttagcgaa tgggatatca tttccaacag
     1741 tgaaacttat tcatataaac tatcgcctgc ccttactgca tttaaagata ccatgcagaa
     1801 attaattgac aaagcaaaag aaattccaca gcattataag ctttctgata tatgcatcag
     1861 tattttgcac gacacacagt acaagaaaag ccttgaagac gaaaatagta ttgaatcaca
     1921 aggaaggctt gaaaatattg atgcatttgt aaacagcatt tttgaatacg aacatctgca
     1981 cccgcaggct acccttaatg aatttttaca ggatatttct ttattaacac ctgctgatga
     2041 aacaaatggc acactgcagg atgccattac ccttatgact gtacacaatg caaaagggct
     2101 ggaatttccg gttgtatttt taactggcat ggaagaaggg ttattccctc atgccaattc
     2161 cagcgataca gaagaaggca ttgaggaaga acggcggctt tgctatgttg gcatgaccag
     2221 ggcaaaggac aggctctttt taac
//
LOCUS       DAORYL010000197         2233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_32656_length_2233_cov_6.621212, whole genome shotgun sequence.
ACCESSION   DAORYL010000197 DAORYL010000000
VERSION     DAORYL010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32656_length_2233_cov_6.621212"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="PLV81_15660"
     CDS             <1..1153
                     /locus_tag="PLV81_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11578.1"
                     /translation="RSTQGFLSSTIAIPINLQTIFPSIKSCSFNYTRASTLNEINVPY
                     EGESINFYEEKYGVSRYLNQCANPIYNIFHYPPWHFFKGRSNYAQGRDFVSHTLSSQP
                     EVNGAPFSDYNNYFRLLDNASFSINWELSPFVLFVNSSIHSVSERNGVNTASQQVVSY
                     NLVSSLSCDLMKLFSFGFFRPNRPDLPYHSATALLEYQWNRYLRITSNILQDEYTPSI
                     GATFKWDRSSIAAKFGISYRTQKWHEFIPLDDNERSARDDIYFYNMMVQSPFTNIDKG
                     YTFSTIYETDVPFIYDFFSTWYTLTALPIFRLEYLMTLNRYNYTYYTSPEPYDLYSVS
                     SSLTINVHKNVQGSCIARGILERYRNRETNDINREIISYELGFQFSLLF"
     gene            complement(1177..>2233)
                     /locus_tag="PLV81_15665"
     CDS             complement(1177..>2233)
                     /locus_tag="PLV81_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11579.1"
                     /translation="QQQENIAIIIKGSPDPDALASSYFFWKLCGILHVKATIFGFMPV
                     SLPSNKEFISVFNIPLRVLADIKEIKLHNYTGYIVCDYQTAVLEENFTTTPCIIHLDH
                     HSPAEHDVPARFRILTDQVSSTSTLCALLMQHISLVSLEDVSSLATALYVGIKTDSDG
                     FKHVGEYDTMALEYIKPYCNMAVIEQIESTPLTPQALSLLQKAMDNAIVYKDWLIAGV
                     GFVEERYRDTIAIIADYLLQDNPVELVVVYGAVYHNNHTKLDIDASLRTRSENFDLNE
                     LIKNITADGGGRKYKGAFQVHIDYFVHCPDQDKLWEIIEITTNQILKNQRDSSLKIVL
                     QSKFKNFKKILKHFFRL"
BASE COUNT          715 a          440 c          354 g          724 t
ORIGIN      
        1 ccgttctaca cagggatttc tatccagtac aattgctata cccataaatc tccaaactat
       61 attccccagc ataaaaagtt gttcattcaa ctatactcgg gcaagcacac tgaatgagat
      121 taatgtcccc tatgaaggcg agtccataaa cttttatgaa gaaaaatatg gagtaagccg
      181 ttatttgaat caatgtgcca accccatcta taacattttt cactatccac catggcattt
      241 ttttaaaggg agatcaaatt acgctcaagg gcgtgatttt gtaagccata cgttatcgtc
      301 tcagccagaa gttaatgggg caccattctc agactataat aactatttca ggctgttaga
      361 taatgcttca ttcagtatca actgggaact atcaccattc gttttatttg taaacagcag
      421 catacattca gtttctgagc gcaatggcgt taataccgct tcccagcagg ttgttagcta
      481 taatcttgtt tcatcattat catgtgattt aatgaaacta ttttcctttg gttttttcag
      541 gccaaacagg ccagatcttc cgtaccattc cgcaactgca ttactagaat atcaatggaa
      601 tagatacctt cgtattactt ccaatatact gcaggatgag tatacaccca gtattggagc
      661 aacatttaaa tgggacagat caagcattgc tgcaaaattt ggtataagct accgtaccca
      721 gaaatggcat gagtttatcc cccttgatga taatgaacga tcagcaaggg atgatatcta
      781 tttttataat atgatggtac aatcaccatt tacaaatatt gataaaggat acacattttc
      841 cacaatttat gaaacagatg tcccgtttat ttatgatttc ttcagcacat ggtatacatt
      901 aacagcatta cctatattca gattagaata tcttatgaca ctcaaccgct ataattacac
      961 ctactatact tcacccgaac catatgacct gtatagtgta tcatcaagct tgacaataaa
     1021 cgttcataaa aatgtacagg gatcatgtat tgcacgcgga atactggaac gctacagaaa
     1081 tagggaaaca aatgacatca accgtgaaat tatatcgtat gaacttggct tccaattcag
     1141 tctgttattt taatataacc agatcttcat atatgattat aagcgaaaga aatgtttaag
     1201 tatttttttg aagtttttaa atttactctg taacacaatt ttcaagctac tatctctttg
     1261 attttttaat atctgatttg ttgttatttc tattatctcc cacaacttat cctgatcagg
     1321 gcaatgcaca aaataatcaa tatgaacctg aaatgctccc ttgtacttgc gtccgccacc
     1381 atcagcagta atatttttta taagctcatt caaatcaaaa ttttcacttc ttgtcctcaa
     1441 cgatgcatca atatccaatt tagtgtgatt attatgatac actgccccat atactactac
     1501 aagctcaact ggattatctt gtaaaagata atccgcaatt atggcgatag tatcacgata
     1561 tctttcttca acaaatccaa ccccagcaat aagccaatct ttataaacaa tcgcattatc
     1621 cattgctttc tgtaacaggc tcaatgcctg aggagttaaa ggagtacttt ctatctgttc
     1681 aattactgcc atattgcaat atggctttat atattccagt gccattgtgt catattcacc
     1741 tacatgttta aaaccatcag aatctgtttt aatgcctaca tatagtgctg ttgccagact
     1801 ggaaacatct tctaatgata ccaacgatat atgctgcatt aataatgcac agagtgtgct
     1861 tgttgacgat acctgatcag ttaaaatgcg aaaacgtgca ggaacatcat gttcggctgg
     1921 agaatggtga tctaaatgga ttatacaggg ggttgtagta aaattttctt ccagaacagc
     1981 tgtctgataa tcgcaaacaa tataccctgt gtagttatgt agtttgattt cttttatatc
     2041 tgccaaaacg cgtagtggta tattaaaaac tgatataaat tctttatttg atggcaggga
     2101 aacaggcata aatccaaata tagtagcttt aacatgtaat attccacata gtttccagaa
     2161 aaaatatgac gaagctagtg catccggatc cggagaacct tttataataa ttgctatatt
     2221 ttcctgttgc tgt
//
LOCUS       DAORYL010000198         2220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_32905_length_2220_cov_6.596305, whole genome shotgun sequence.
ACCESSION   DAORYL010000198 DAORYL010000000
VERSION     DAORYL010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32905_length_2220_cov_6.596305"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            380..631
                     /locus_tag="PLV81_15670"
     CDS             380..631
                     /locus_tag="PLV81_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11580.1"
                     /translation="MINKISQLLIIATIMISTNAFALDLNVGATLWYANWKPHWEKEF
                     RDIFRDESGEKTHFKMDDTYLIGPIVSLKLGDTFSQVQT"
     gene            703..1251
                     /locus_tag="PLV81_15675"
     CDS             703..1251
                     /locus_tag="PLV81_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11581.1"
                     /translation="MDITKYDTDIILNYRAFTYVQFFVGLKFQHYNLTETQRGIYDTP
                     PCPFSRELLSNSYGPGLGIGLTIPLDENFAMLLNISGIYLRTKVEDSWKENSGANPPS
                     YIYTLNAYGINNSLSLAYFLPSFNITLLTGFRYQYLKYKQADVKKTNYDPGDEFRFDR
                     DYNDQRDIFWGINISVLYSFSI"
     gene            complement(1262..>2220)
                     /locus_tag="PLV81_15680"
     CDS             complement(1262..>2220)
                     /locus_tag="PLV81_15680"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOM11582.1"
                     /translation="SPQLVDDAVKAYAGKGIEVVVSIGGGSVIDAGKAITAMLPVGGS
                     VTDFLEVVGTKQHNGVKVPFIAVPTTAGTGSEATKNAVLSKTGKNGFKRSLRHDNFVP
                     NIAIIDPELMLGCPSGVTAASGLDALTQLIESYVSTQANPFTDALAESGIAKIGHSLL
                     PLCIHHEDTVFHRANMAYGAFISGITLAHAGLGVVHGLASPLGALFNIPHGVACGTLL
                     ASATKATIKKLQALGDSYYIKKYAAISELLTGYKSTSDQEACNKLIATLEEWTDILHM
                     PRLSEYGVREVDFAAIIKDGSNKNNPAPLNEQEIMLMLQERL"
BASE COUNT          699 a          431 c          395 g          695 t
ORIGIN      
        1 caagcatatt gtgcattacc ttaaagggtt tcctggtgca agcaaactaa ggcaggaaat
       61 aattagctgt gatagcatag aggaaatttt caagatactc gatagttctt ttgattaaga
      121 atgttcacga ataggataat aagagcctat ttacaataac aaatgatgat ttaactttat
      181 attgacattt gcaaagattt atttataatt cttacaatat atgtttatta ttttatagta
      241 tactataata taaagatata atttcagatt gggcacattc cacaccgtaa accattctgt
      301 taatattctg tattatacaa taatcaaata aaataatatt cactattgac tttaaatttt
      361 cagatgaagg ggatacgtta tgataaataa aatatcacaa cttttaatta tagctactat
      421 catgatttcc actaatgcat ttgcattaga tttaaatgtt ggtgcaacat tatggtatgc
      481 taactggaaa cctcactggg aaaaagaatt tcgggatata ttccgcgatg aatccggtga
      541 aaaaacacat tttaaaatgg atgatactta tttgattgga ccaatagtgt cattaaaact
      601 tggtgataca tttagccagg tgcaaacatg atctatggga aatacactgc ttcatgtatt
      661 aaaatgtggt atgaaggagg cacagaatca atccattcaa caatggatat cacaaaatat
      721 gatacagata tcatactaaa ttacagagca tttacttatg tacaattctt tgttgggctt
      781 aagtttcaac actataatct tactgaaaca cagagaggaa tatatgatac tcctccatgc
      841 ccattttccc gtgaattatt atcaaacagt tatggtccgg gtttaggcat tggattgaca
      901 attcctttag atgaaaattt tgccatgctt ttgaatattt caggaatata tctgagaaca
      961 aaggttgaag attcgtggaa ggaaaactca ggtgcaaatc caccatcata catatacacg
     1021 ttaaatgcct atggcattaa taattcattg tcattagcct attttcttcc ctcatttaat
     1081 ataacattac tcacaggttt tagatatcaa tatcttaaat ataaacaagc tgatgttaaa
     1141 aaaaccaact atgatccagg ggatgaattc agatttgata gagattataa tgatcagagg
     1201 gatatttttt ggggtataaa tatttctgtt ttatatagtt tctctatcta agaaaaaaat
     1261 tttataatcg ttcctgtaac atgagcatta tttcctgttc attaagtggt gctggattat
     1321 tcttattgct accatcctta attatcgcag caaaatctac ctcgcgcaca ccgtattcag
     1381 acaggcgtgg catgtgcaaa atatcagtcc actcttctaa tgtggcaata agcttattgc
     1441 aggcttcctg gtcactggtg cttttgtagc ctgttaaaag ctcacttata gcagcatatt
     1501 tttttatata gtaactatcg cccaatgctt gtaatttttt tattgtagct tttgttgctg
     1561 aagctaacag tgtcccgcag gcaacaccat ggggtatgtt aaacaatgca ccaagcggtg
     1621 acgccaaccc atgcaccacg ccaagccctg catgcgcaag cgttatgccc gatataaatg
     1681 cgccgtacgc catattagcg cgatggaaca ccgtatcttc atggtgaata catagtggca
     1741 acaaactgtg cccaatcttt gcaataccac tttcggccag agcatcggtg aaaggatttg
     1801 cctgtgttga cacatacgac tcaatcaatt gcgtaagcgc atcaagtccg ctcgctgcag
     1861 tgactccgga agggcaccca agcataagct ctggatcaat gatagcgatg ttgggcacaa
     1921 aattgtcatg cctgagagag cgcttaaacc cgtttttgcc agttttgctt aatactgcat
     1981 ttttggttgc ctcacttcca gttccagctg tggtgggcac tgcaataaaa ggaaccttga
     2041 cgccgttgtg ctgctttgtg ccaacaacct ccagaaaatc ggttacactt ccaccaacag
     2101 gtaacattgc tgtaattgct ttacccgcat caattacact tcccccgcca attgacacca
     2161 ccacttcaat gcccttgcct gcatatgctt tcaccgcatc atcaacaagc tgcggtgaag
//
LOCUS       DAORYL010000199         2184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_33650_length_2184_cov_14.786754, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000199 DAORYL010000000
VERSION     DAORYL010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33650_length_2184_cov_14.786754"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /gene="murC"
                     /locus_tag="PLV81_15685"
     CDS             <1..207
                     /gene="murC"
                     /locus_tag="PLV81_15685"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the formation of
                     UDP-N-acetylmuramoyl-L-alanine from UDP-N-acetylmuramate
                     and L-alanine in peptidoglycan synthesis; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOM11583.1"
                     /translation="EGVSSQLIQQAVKKHEGRDVEIIQHLDDVPEYVCRIARKGDVIL
                     TLGAGDIYKVGPKILSVIEGKGIR"
     gene            204..890
                     /locus_tag="PLV81_15690"
     CDS             204..890
                     /locus_tag="PLV81_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11584.1"
                     /translation="MKVAFYIWIMILFCGVIPTVTYADVVQVTHVRFQGLKYVTQHEI
                     LQHGVFMKDGKIFVDVAVLNEELKKNIMIESYNITIEKNICTIQVVEKDILAIILYDK
                     GDSLVSVEVDANLNIIPTSRVYAYDRPIIKINQAEVINNKLSPRIIRILIILKNMIQN
                     NAIVSRISVINCTDPEVSYVQFHGFTSRCIWDETYNGLSLLDAVVGVYDSKKAYPGNA
                     MVTQKRMVVW"
     gene            896..2137
                     /gene="ftsA"
                     /locus_tag="PLV81_15695"
     CDS             896..2137
                     /gene="ftsA"
                     /locus_tag="PLV81_15695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOM11585.1"
                     /translation="MKEDTILDNIVVGLDIGTTKTCAVIGFLNENKQVEVVGVGVAPS
                     RGLKNGVIVNIDNTVSSIVKAIEDAELMAGCEVSSVFVGVTGQHIKGENSRGVVAVAN
                     RSRTITPLEMKRVIEAAQAIVIPMDREIIHVLSKEFAVDDQTGIKDPIGMTGVRLEAE
                     VHIITGSTTSIQNMVKSINKAGFTCNDIVFSPLASAEATLSRDEKDLGVALVDIGGGT
                     TDIIIFIEGGVAYSAVLGIGGIHVTNDISIGLRTPIDSAEVIKKKYGCAVVDLVDASE
                     TIEVPSVGGRAPRRLFRHELAQIIEPRMIEIMEMIDNELVKSGKKEILAAGVVLTGGG
                     SMIEGTVEAAERVFGMPVRVGVPENIAGLKDVVSTPVYANGVGLLKYGAKMSQVRQTR
                     KTKSAFTSFKDKLKHIFNDYL"
BASE COUNT          695 a          303 c          546 g          640 t
ORIGIN      
        1 gaaggtgtca gcagccagct tatacagcag gcggttaaga agcatgaagg aagagatgtg
       61 gaaattattc agcacttgga tgatgttccg gaatatgtat gcaggatagc cagaaaaggt
      121 gatgtaatcc tgacgctggg tgctggtgat atttataaag ttgggccaaa gatactttca
      181 gtaatagagg gtaaagggat aaggtgaagg ttgcttttta catctggata atgatactgt
      241 tttgtggagt tatacccacc gtaacgtacg cagatgtggt gcaggtaact catgtgaggt
      301 ttcagggatt gaaatatgtt acccagcatg aaatattgca acatggcgtt tttatgaaag
      361 atggaaaaat tttcgtagat gttgctgtgt tgaatgaaga gttgaagaaa aatatcatga
      421 ttgaatcgta taatattact atcgaaaaga acatatgtac aatacaggtt gttgaaaagg
      481 atatcctggc tattattctc tatgataaag gagattctct tgtttcagtt gaggtggatg
      541 caaatctgaa tattatccca accagtagag tttatgccta tgaccgaccg ataattaaaa
      601 taaaccaggc agaggttatt aataataaac tatcgccaag aataatacgt attcttatta
      661 ttttgaaaaa catgatacaa aacaatgcca ttgtaagcag aatttcggta attaattgca
      721 cagatcccga agtatcgtat gtgcaatttc atggttttac atcacgatgt atatgggatg
      781 aaacatacaa tggcctttca ttacttgatg ctgttgtagg agtatatgat agtaaaaaag
      841 cttatcctgg taatgccatg gtgacacaaa aacgaatggt ggtatggtga cagatatgaa
      901 agaagatacc atattagata acattgttgt agggctggat attggaacaa caaaaacctg
      961 tgcagtcatt ggctttttga atgaaaataa acaggttgaa gttgtaggtg ttggggttgc
     1021 gccttcacga ggtttaaaaa atggcgttat agtaaatatt gataataccg tttcttcaat
     1081 tgtcaaagcg atagaagatg cagagttaat ggctggttgt gaagtaagtt cggtatttgt
     1141 tggtgttacc gggcagcata taaaaggtga aaactcaaga ggtgtggttg ctgttgcaaa
     1201 ccgaagccgt acaatcacgc cacttgaaat gaaacgggtt attgaagctg cacaggctat
     1261 cgttattccc atggatagag agataatcca tgttctatcc aaagaatttg ctgttgatga
     1321 tcagacaggt ataaaggatc ctataggaat gactggtgta aggcttgaag ctgaagtgca
     1381 tattattact ggctctacta caagcattca gaatatggtg aaatctatta ataaagctgg
     1441 cttcacatgt aatgacatag tattttcgcc tctggcttct gctgaagcaa cgttgagccg
     1501 tgacgaaaag gatttaggtg tggcgcttgt ggatataggt ggtggcacta cggatatcat
     1561 catttttata gaaggtggtg tggcatattc ggcagtattg gggataggcg gcatacatgt
     1621 tactaatgat atatccatag gattaaggac gccaattgat tctgcagaag ttatcaagaa
     1681 gaaatatgga tgcgcagtgg tagatctggt tgatgcttct gagacaattg aggtgccatc
     1741 ggttggtgga agggcaccgc ggagattatt caggcatgaa ctggcacaga ttattgaacc
     1801 gcggatgatt gaaataatgg aaatgattga taatgaactg gtaaaaagtg gtaaaaagga
     1861 aatactggca gctggtgtgg tgttgactgg tggcggcagc atgatagaag gtaccgtgga
     1921 ggcagccgaa agagtatttg gaatgccagt acgtgttggc gtcccagaga atattgccgg
     1981 attgaaagat gttgtgtcaa ctcccgtgta tgccaatggt gttggattat tgaaatatgg
     2041 tgctaagatg agccaggtga ggcaaacacg caaaactaaa agtgctttta caagttttaa
     2101 ggataaactc aagcatattt ttaatgatta tttataatac agtcgttgca gttgaatttt
     2161 ttattaattt tttttaaaaa ataa
//
LOCUS       DAORYL010000200         2166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_34061_length_2166_cov_13.673614, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000200 DAORYL010000000
VERSION     DAORYL010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34061_length_2166_cov_13.673614"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PLV81_15700"
     CDS             <1..714
                     /locus_tag="PLV81_15700"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOM11586.1"
                     /translation="QGVANDKDLKIMHHSKNRGYGAALKTGIRAAKHDFIAITDADAT
                     YPDERIPEFFKDVITNDLDMLVGARIGESVNIPLIRKFPKWVINQLANYMVGTKIPDL
                     NSGMRIMKKSVVEKFMKILPEGFSFTSTITIAMLANDYQVKYVPIDYHQRKGKSKIRP
                     FYDTLNFVRLIVRTVMFFDPLKVFLPLSLPLLLCGVAMVLIEGILYRNINTVSVLITL
                     SGLNILTVGMLAEMIAHKE"
     gene            complement(722..1813)
                     /locus_tag="PLV81_15705"
     CDS             complement(722..1813)
                     /locus_tag="PLV81_15705"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOM11587.1"
                     /translation="MKHLISIIVPTYKEAENIPVLIESIDKAMKHSRLDYEVIIVDDN
                     SKDGIEDNVKALKQKGYPVSIKVRINERGLSSAVIEGFRQAKGTIYCVMDADLSHPPE
                     KIPELVEQIINGKAEFVVGSRFVKGGSAEHFNWYRKLNAWVSKMLARPFTKIKDPMAG
                     FFAFDAKILTPHILNMLNPLGFKIGLEIIVKCNPRTITEIPISFRERLFGQSKLSLRE
                     QILYIVHLKRLFHYKYPSLYQFLFFSLIGSTGMVVDMTTVFVTYGLLHINFKIARAIG
                     FIVALTWNFFLNRKLTFPDSPQKNLFIQYRNFFIVCSIGGLFNWFISVYLYSTYPYFK
                     EFYLLAVFIGIMGGLLINFLGSKYIVFGK"
     gene            1919..>2166
                     /locus_tag="PLV81_15710"
     CDS             1919..>2166
                     /locus_tag="PLV81_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11588.1"
                     /translation="MLPNNPVKSFYEIFQRPTVFTFFFIAIVIVILRLPSVIIDFIHV
                     DVLTSYLLAKRELLGLSFGMNKGWLYHWLMKFSITYIA"
BASE COUNT          683 a          392 c          393 g          698 t
ORIGIN      
        1 cagggtgttg caaacgacaa agatttaaaa atcatgcatc attctaaaaa tcgtggttat
       61 ggtgcagcac tcaagactgg tatcagagcg gcaaaacacg attttattgc cattactgat
      121 gcagatgcca catatcctga tgaacgtatc cctgaatttt ttaaggatgt tattacaaat
      181 gaccttgata tgcttgttgg tgcaagaata ggtgaatctg tgaatatacc tttaatacgc
      241 aaattcccca aatgggtcat aaaccagctg gcaaattata tggtaggaac aaagattcct
      301 gaccttaact caggcatgcg aattatgaaa aagtcagtag ttgaaaagtt tatgaaaatt
      361 cttcctgagg gtttttcttt tacctctact atcaccatag ctatgcttgc taatgattat
      421 caggtaaaat atgtacctat cgattatcat cagcgtaaag ggaaatcaaa gataagacct
      481 ttttatgata cattgaattt tgtcaggctt attgtgcgta ctgttatgtt ctttgatcct
      541 ctcaaagttt ttctgccctt aagcctccca ttactgttgt gtggagttgc aatggtcctg
      601 atagaaggta tactctatag gaatattaat acagtatcag tacttatcac gctgtcgggt
      661 ttaaatattc tgacagttgg catgcttgcg gaaatgattg ctcataaaga ataattttgg
      721 ttcatttccc aaaaacaata tatttggaac ccaggaaatt aatgagtaat ccccccatga
      781 ttcctataaa cacggccaga agataaaatt ctttaaaata tggatatgtg gaatacaggt
      841 aaacagaaat aaaccagtta aaaagcccac caatactgca gactatgaaa aaatttctgt
      901 attggatgaa aagatttttt tgcggcgagt cagggaatgt cagtttcctg ttaaggaaaa
      961 agttccatgt tagtgcaaca ataaatccaa tagcccgtgc aatcttgaaa ttaatgtgta
     1021 acaatccata tgtaacaaat actgtggtca tatcaactac catacccgtt gacccaatca
     1081 gggaaaaaaa tagaaactgg tacagcgatg gatatttata atgaaacaaa cgtttcaaat
     1141 gcactatata cagtatctgt tcacgtaatg aaagcttgct ttggccaaaa agccgttctc
     1201 tgaatgatat gggaatttct gttattgttc tggggttaca tttcacaata atttccaggc
     1261 caattttaaa accaagcggg tttaacatat taagaatgtg aggggtgagt attttagcat
     1321 caaaagcaaa aaatcctgcc atgggatcct ttatctttgt aaagggacgc gctaacatct
     1381 tggataccca tgcattgagc tttcggtacc agttgaaatg ctcagctgag ccacctttaa
     1441 caaatctgct accaactaca aattcagctt taccattaat gatttgttca acaagctcag
     1501 gtattttttc gggtggatgg ctcaaatcag catccatcac acaatagatg gtgcctttag
     1561 cctgtctgaa cccttcaatg acagctgaag aaagtccacg ttcgtttatg cgtactttta
     1621 tagaaacagg gtagcctttt tgcttaagtg ctttaacatt gtcctcaatt ccatctttag
     1681 aattatcgtc cacaataata acttcgtaat caagcctgct gtgcttcatt gctttatcaa
     1741 ttgattcaat gagaacagga atattttctg cttctttgta cgttgggaca ataatggaaa
     1801 tgagatgttt catatgatat tcctctttgg gtttaataaa aaactaattg actccagcac
     1861 aggtgtttac acactatttt cctattaaaa atagtcaatt gatttatgga gaaaatactt
     1921 gttgcccaat aatcctgtca aatcttttta tgaaatcttc cagcgtccta cagttttcac
     1981 tttttttttc atagcgatag taattgttat actacggttg ccatcagtaa ttatagattt
     2041 tatccacgtt gatgttctta cctcatattt actggcaaaa agagagttgt tgggcctatc
     2101 atttggtatg aacaaggggt ggctgtatca ctggcttatg aaattttcca ttacatatat
     2161 agccga
//
LOCUS       DAORYL010000201         2144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_34510_length_2144_cov_7.559598, whole genome shotgun sequence.
ACCESSION   DAORYL010000201 DAORYL010000000
VERSION     DAORYL010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34510_length_2144_cov_7.559598"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PLV81_15715"
     CDS             complement(<1..462)
                     /locus_tag="PLV81_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11589.1"
                     /translation="MYTSCNYACQPGFNASCALCCGSHNYTASFHDIDALFKDRFKFF
                     SNMLAKSNDIVSALKACYQELSHITLPAIHPDATQCPFVAYTDSSHTIIGCLLYPYCT
                     NFDYRSLFMGAICKTFSCSVNTVHYADRIQTAAKRCGDWYYYSLIIHDMDLL"
     gene            602..1357
                     /locus_tag="PLV81_15720"
     CDS             602..1357
                     /locus_tag="PLV81_15720"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOM11590.1"
                     /translation="MKGQYFSTTFGPMYYRTKDGDANIHLVCIHGAGGDSRLFIPLMN
                     EINNITVHAVDLPAHGKSKIQECSLDIYMQATMQFIQSLQGNVFVLGHSMGGGIIFEL
                     VKHDAPVKGALFLATAGTLPVNPVVFELLDKDFDSLCRLAVDLSYGSADETIRTTAIE
                     YMKQAGSSILKNDFTICNNYNYEEDAKSFKQAACFIANRKDKMVPLDLTYTTFKKMPN
                     AMLQVFPYKGHMLHIEQPSQVSRCIEQFVELSL"
     gene            1484..>2144
                     /locus_tag="PLV81_15725"
     CDS             1484..>2144
                     /locus_tag="PLV81_15725"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOM11591.1"
                     /translation="MSIKVAINGFGRIGRMVLRIIMSDPKYADIDVRCINDLTDAKTL
                     AVLFKYDSVFGIFNGTVEHTDNSIIVNGKEIKILAEKNPGSLPWKAEGIDVVVESTGK
                     FRDRDKVMPHLDAGAKKVIITAPAKGEDITIVLGVNEDKYDPSKHHIISNASCTTNCL
                     APVVKVLNDTFGVEKGVMTTVHSYTNDQRLLDLPHSDLRRARAAALSMIPTTTGAAKA
                     VA"
BASE COUNT          689 a          358 c          456 g          641 t
ORIGIN      
        1 taaaagatcc atgtcatgga taatcagact atagtagtac cagtcgccac aacgttttgc
       61 tgctgtctgt attctgtcag catagtgtac tgtgtttaca ctgcagctaa atgttttgca
      121 gatagctccc atgaaaagac ttcgataatc aaaattggta cagtaagggt aaagcagaca
      181 accaataatt gtatgtgagc tgtcggtata tgctacaaaa gggcattgtg ttgcatcagg
      241 gtggatagcg ggaagagtta tgtgcgatag ttcctgataa caggctttca gtgcagatac
      301 gatatcatta cttttagcaa gcatattgct gaaaaattta aaccggtctt taaataaagc
      361 gtcaatgtca tgaaatgatg ccgtatagtt gtggctgcca cagcacaacg cacacgaagc
      421 attaaaacct ggttgacacg cataattaca tgaggtatac ataaatcaat tattcgttta
      481 ttatagtagt atgccgtatt aagcaaatta tagctcgaca atttgcgtgt atacatgtat
      541 tgttttatac tatagccaaa aagatgaatt gtatataatt taattgcagg ggaaatacat
      601 aatgaaaggc cagtattttt caactacatt tgggcccatg tactatcgca caaaagatgg
      661 tgatgctaat attcatcttg tatgtataca tggtgcagga ggagattcgc gcttatttat
      721 tccattaatg aatgaaataa acaatataac ggtgcatgct gtagatcttc ctgctcatgg
      781 aaaatctaaa attcaggagt gctcactgga tatatatatg caggcaacga tgcaatttat
      841 acagtcttta cagggcaatg tctttgtgct tggccattca atgggtggtg gcataatttt
      901 tgagctggtg aaacatgatg cacccgtaaa aggtgcgctg tttttagcaa cagctggcac
      961 gctgccagta aaccctgttg tatttgaact gttggataag gattttgata gtttatgccg
     1021 tctggcagtt gacctgagct atggtagtgc ggatgaaacc atacgcacta ctgcaattga
     1081 atatatgaag caggcaggta gcagtatatt aaaaaatgat tttacaatct gtaataacta
     1141 taattatgaa gaggatgcta aaagttttaa acaggcagcc tgctttattg ctaacaggaa
     1201 ggataaaatg gtgccactgg atttaacata tacaacgttt aaaaaaatgc ccaatgccat
     1261 gttgcaggta ttcccctata agggacatat gctgcatatt gaacagcctt cacaggtatc
     1321 acggtgcatt gaacaatttg tggagctatc gctttaaaaa tttttaagta gttttaaaaa
     1381 aaattatgtg taaaatagta aaaaatagta tatattgatt tgtgtagtca tagtatcttc
     1441 tttgttggtg taaatttaaa actaaatata tcaggaggtt gttatgagta tcaaggttgc
     1501 tatcaatggt tttggccgta taggaagaat ggtattgcga ataataatga gcgatccaaa
     1561 atatgcagat attgatgtgc gctgtattaa tgatttaacc gacgctaaaa cccttgcagt
     1621 acttttcaaa tatgattcag tatttggcat ttttaacggc acggtggaac atactgataa
     1681 ttcaataatt gtgaatggta aagaaattaa aatcctggct gaaaaaaatc caggctcgct
     1741 gccatggaaa gctgaaggta tagatgtggt tgttgaatct accggtaaat tccgagaccg
     1801 tgataaggta atgccgcatt tagacgcagg tgcaaaaaaa gttatcatta ctgcaccagc
     1861 aaagggtgag gatatcacta ttgtactggg tgtcaatgaa gataaatatg atccatccaa
     1921 acatcatatt atttcaaatg catcatgtac tacaaactgt ctggctccag tagtcaaggt
     1981 gctcaacgat acatttggtg ttgaaaaagg tgtgatgacc actgttcact catacaccaa
     2041 tgaccagcgt ttgcttgatt tgccacactc tgatttgcgc agagcacgtg ccgccgcact
     2101 ttctatgata cccacaacaa caggtgcagc aaaggcagtt gcgc
//
LOCUS       DAORYL010000202         2136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_34712_length_2136_cov_15.042287, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000202 DAORYL010000000
VERSION     DAORYL010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34712_length_2136_cov_15.042287"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..1389
                     /locus_tag="PLV81_15730"
     CDS             112..1389
                     /locus_tag="PLV81_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11592.1"
                     /translation="MVEKLIRYILLAAVISISMVLFAQDKPQEKKSSIGKTIKKTIET
                     IDKQTLYKEKKETEQDKAEKKQIQTYQQADFDKLKDATHEDISTIIVLLGEIPLESKN
                     SIFAPAILYNKNNIFNSNTDFAFTWVGFKATAKFTQKKFIWDNVSLQETLIGSFLYAS
                     GTNFGFFGERFNEDLLFYTNYTSEIVTLKISLPLYNILGLTIDSRQYFFIKRDTPPDF
                     IMPKNHYNLFPRIDWNIEHYTEQGIDQLFNGIAFQNWIGYGIRSQWDTWGEPGKLQMG
                     KEAKTFVIYSSTLTCGKVFGNSQNLIVRMRIKGGIDNDFLSQPRFGGTIDNAKLDVVH
                     GTTVDQFRVESFALCNVQYGFNVATRLRMNLYVDYAYIINPASQHIAGSGYGFRILGP
                     GGLPIWLTHGISKNLSQDDKVSQVVMIMTAAGF"
     gene            1498..1962
                     /gene="ribE"
                     /locus_tag="PLV81_15735"
     CDS             1498..1962
                     /gene="ribE"
                     /locus_tag="PLV81_15735"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOM11593.1"
                     /translation="MNTIEGKLDASGLKVAIIVSRFNEFITNKLLSGALDCLQRHNAD
                     EKNITVIWVPGAFEIPPVAMKVATKGNYDAVICLGAVIRGATPHFDYVAAEVSKGVAL
                     VSLQSQVPVIYGVLTTDTIEQAVERAGTKSGNKGFDAAMAAIEMAQLYKKIS"
BASE COUNT          709 a          377 c          430 g          620 t
ORIGIN      
        1 tttatacaat tgtcttagat aatgaaaggt acaatcctgt caccagtgat ggcaggtata
       61 tcgtaaacag gtattgtaaa taaattacaa aattacaact ttcaggaatt catggtggaa
      121 aaattaatac gatacattct gttagctgca gtcatttcca tttcaatggt actatttgca
      181 caggacaaac ctcaagaaaa aaaatcttct attggcaaaa ctattaagaa aacaattgaa
      241 acaattgaca aacaaacatt atacaaagaa aaaaaagaga ccgaacagga taaagcagaa
      301 aaaaaacaaa tacaaacata ccagcaggct gattttgata aattgaagga tgctacccat
      361 gaagatatct caaccattat agtattgctg ggtgaaatac cactggaatc taaaaatagt
      421 atttttgccc ctgctatcct ttataacaaa aacaatattt ttaactcaaa tacggatttt
      481 gcttttacct gggttgggtt caaagctact gcaaaattta cccagaaaaa atttatatgg
      541 gataatgttt cattacagga aacattaatt ggatcatttt tatatgccag tggtacaaac
      601 tttggtttct ttggcgagcg ctttaatgaa gacctcctgt tttatacaaa ctacacatca
      661 gagatagtta ccttaaaaat ttctttgcca ttatataaca tcctgggctt aacaattgat
      721 tcccggcaat acttctttat aaagcgagat acaccccctg attttattat gccaaaaaac
      781 cactataatc tttttccgcg cattgattgg aatattgagc actataccga acaggggatt
      841 gaccaattat ttaatggcat tgcatttcag aactggattg gatatggaat ccgcagccag
      901 tgggacacgt ggggtgaacc cggcaagctt cagatgggga aagaagctaa aacctttgtt
      961 atttattcat caacattaac ctgtggcaaa gtctttggta acagccagaa cctcattgta
     1021 cgcatgcgca ttaaaggtgg cattgataat gactttttat ctcagccacg ctttggcgga
     1081 accattgaca atgccaaatt ggatgtggta catggcacca cggtggatca gttcagagta
     1141 gagtcttttg ccctgtgtaa cgtgcagtat ggatttaatg tggcaacacg gttacggatg
     1201 aatctttatg ttgattatgc ttatattata aaccctgcat cacaacacat tgcaggcagc
     1261 ggctatggct tcagaatact gggacctggg gggcttccta tctggttgac acatggtata
     1321 agcaaaaatc tgtcacagga tgacaaggtt agtcaggtcg ttatgataat gaccgctgca
     1381 ggtttttaaa aaataacttg acggaataga atatattgaa acaaactttg tcatgcatta
     1441 gatttattat tataagcttt agcccaaatt aaaatttatt atataagcag gttaactatg
     1501 aacacaattg aagggaaatt agatgcatcc ggtttaaagg ttgcgataat agtttcacgt
     1561 tttaatgaat ttattaccaa taagctttta tccggcgctt tagattgcct gcaacggcac
     1621 aatgctgatg aaaaaaatat aactgttata tgggtacctg gcgcgtttga aattcctccg
     1681 gtagcaatga aagtggccac taaaggcaat tatgatgcag taatctgcct gggagcagtt
     1741 atacggggtg ctactccaca ttttgactat gttgcagcag aagtttcaaa aggtgttgcg
     1801 cttgtatcgc tgcaatcgca ggtgccagtt atctatgggg tgcttactac cgataccatt
     1861 gaacaggctg ttgagcgtgc aggaacaaaa tcaggcaata agggctttga cgctgcaatg
     1921 gcagctattg aaatggcaca actgtataaa aagataagct aatgggtcat agaagaaaag
     1981 cccgtgaata tgccatgcag gcgctgtata tgtacgaaac ggttcaaaca ccgttacagg
     2041 agcttattgc tttacaatgg atagataagg aaattccgga agatatcagg gattttgcca
     2101 ttacattaat tgaaggcact atagaacatt tagatt
//
LOCUS       DAORYL010000203         2119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_35107_length_2119_cov_4.713178, whole genome shotgun sequence.
ACCESSION   DAORYL010000203 DAORYL010000000
VERSION     DAORYL010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35107_length_2119_cov_4.713178"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..611)
                     /locus_tag="PLV81_15740"
                     /pseudo
     CDS             complement(225..611)
                     /locus_tag="PLV81_15740"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
     gene            complement(627..779)
                     /locus_tag="PLV81_15745"
     CDS             complement(627..779)
                     /locus_tag="PLV81_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11594.1"
                     /translation="MKKACVTMFVVLLVFPVAVFAAEQNVALSAGLGVVYPMGDLGDV
                     IDYAIS"
     gene            1412..1741
                     /locus_tag="PLV81_15750"
     CDS             1412..1741
                     /locus_tag="PLV81_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11595.1"
                     /translation="MTFMDIVYDMMQKVNTIAKENNYNRYIKKHPVEKMFWVVLFQQY
                     MGLQNGRSLLLQLKYILGIDNQNIPSQGELSKKLSYLLPVGLWEQLFRIYVGECGSCI
                     QHLFRIY"
BASE COUNT          697 a          350 c          320 g          752 t
ORIGIN      
        1 gctgattgat gccatcatca ttaactttat caaaatcatt aaaccctaaa tgggtgtcag
       61 aaaggtgtat aaatactggc ataataaaac ccttaattaa acatatagta tttaaacaag
      121 tgatgcataa acaattgtca accacattaa aaacaaaagg ctgcatgatt gcagcccgta
      181 aaataatatc tttgcaaata taccagaaca ctaatacaaa ataactacat tgacaatgga
      241 tatgatgcca ttacatataa tgtcaacggc tggtcaaacc aatcatccat atcaatagca
      301 tcataccgga tacccgcatc aagtattatt gggtcaagct tcatagcata tccagctttt
      361 aaaaacaaag ttgtataatc atcttttgtt tttgtagtat aggtgccaaa gaatggaata
      421 gtatatttca gttctgattc aaaatgtgtt cttccaagac ctgcaccaaa atatatccca
      481 ttgagtgctt tttccaacca gtaacgagca cctattccat attgatacat agtaaaactg
      541 tctaaatctg ttgaagtgta gagataatag tacccatcaa gctccacaga aacatttgga
      601 acaacagcat actgtgcccc tactggctag gaaatggcat aatcaataac atccccaaga
      661 tcacccattg gatatactac acccagacca gctgaaagtg ctacattctg ttcagcagca
      721 aaaactgcta ccgggaaaac cagtaaaaca acaaacatcg taacgcatgc ttttttcatg
      781 tattcctcct tacttttttt aattactttt gtagttctgg tatctttatt ttattttttt
      841 tggataaata aaaatatgtt aaatattttt gtcaatcttt tttttattta taaaattatc
      901 tttatccagc ttcaagtttg aagtatacta cgccgtacct cctgtacggc taccattgcc
      961 ccttactttt gcttttatcc acctttgctg tttttaaaag aacgctttga tctggcttat
     1021 gcctttgggt gtgctttttc ttctgcttca tattcattat atccatatgt tgcattctgc
     1081 tttatttctc tgctaattgt tgaatgatgt ctctttaacc tacacgatat ttcacgtaaa
     1141 gatattcctt tgctataaag cattgttttg atgacaattc tttctcttca ttgtatatga
     1201 gtataatgcc tcatacatac ctgcttttta gtttttgttg ttggaacatc cactctaaag
     1261 gatgtgttgt gtatgtggct acatgttttc tattttgtgt tgcacttcac caattattcc
     1321 taaacaataa aaaataaaaa aatgctatta atagaaaagc ctccttatac aatatattat
     1381 ctaaatctca cgcatataag gaggttatca aatgacattc atggatattg tctatgatat
     1441 gatgcaaaaa gtcaacacta ttgcaaagga aaacaattat aatcggtata ttaagaagca
     1501 tccagtagag aagatgtttt gggtagtatt atttcagcaa tacatgggat tacaaaatgg
     1561 gaggagtttg ttgttacaac tgaaatatat acttgggata gataaccaga atatacccag
     1621 tcagggtgaa ttatcaaaga aactaagcta tctattacct gttggattat gggaacaact
     1681 atttaggatt tacgtaggcg aatgcggaag ttgtattcag catttatttc gtatttatta
     1741 gtattgcttg cacaaatgag tgacgccaca cctataagag tgttgaggga ttatgtgtat
     1801 ccgtattctc agctttttat gcgtatcatt tgtgtttcaa aatattataa acattctgaa
     1861 aatattttac atttttcagg ttaaggaatt aatggtgaaa ataaataata aattaataaa
     1921 taaatttata attttattat catgaaaaat catagtatta tgataaattg aaatgactcg
     1981 tcagtttttt tattttgttg tttaggtttt ttatacaaca taaaataatt ttatataggc
     2041 atctgcctaa gaaaaatcct tctggactga atatttttgt tgatttttta aagcatttat
     2101 atttagtatg gtgttgttt
//
LOCUS       DAORYL010000204         2086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_35911_length_2086_cov_14.003939, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000204 DAORYL010000000
VERSION     DAORYL010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35911_length_2086_cov_14.003939"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..1030)
                     /gene="fliG"
                     /locus_tag="PLV81_15755"
     CDS             complement(14..1030)
                     /gene="fliG"
                     /locus_tag="PLV81_15755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOM11596.1"
                     /translation="MKPIDQMNGAEKAAALLVALGPEVASEVMKFLDEESVNKLAAEI
                     AKIDVLSPEEKEELIGQFLLELRKARGVAFGGQNVAKEILYAAFGEDKAKDILKRLTH
                     KDLEKGFGFLNDIDAELIVTFLQNEHPQTIAVTLAYIPAAKAAEVIKLLPKDLAKEVT
                     LRMAKMERTSPDAVLEISRVLRKKYDEYRYQGQSSSKAGGLNALIDIMAHLSGDQEKQ
                     LIEYFETTMPQIADEIRSRIFTFENVLTLTNTEMRILIDEINNDYMIAKALKGAGDEI
                     RFKFFRNMSKNRATDIMQEMEAMGPVRLSEIQEARDNIVRLMRQLHDNGVISLRKEKE
                     KYIE"
     gene            complement(1017..1880)
                     /locus_tag="PLV81_15760"
     CDS             complement(1017..1880)
                     /locus_tag="PLV81_15760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOM11597.1"
                     /translation="MLDILFPSVPSIISITVLSLACGILLSLAKVLLKVEKDPRVEKV
                     IEALPGANCGACGFPGCAGYATNIVEHGAAINMCPVGGPESVKKISEIMGIEAEAGVK
                     RIARVHCQGGVNETKIRFMYDGPHSCRAAQLVMGGFKVCEYGCLGLGDCVRACPFDAI
                     HLDDRGLPFVDAEKCTGCGNCVEACPRDIISLLENTVDVYVICRNKQKAPVMKLGCSV
                     GCTACKRCEKACREEVFKDNPDIETAIKVENFLAGIDYELCINCGRCAEVCPQKVIDF
                     KKIMVIKREAN"
BASE COUNT          581 a          509 c          346 g          650 t
ORIGIN      
        1 aaggatttct tttttattca atatacttct ctttttcttt tctgaggctg atgacaccat
       61 tgtcatgcaa ttgacgcatt agtctgacaa tattgtcccg cgcttcctgt atctcggaca
      121 ggcgcacggg gcccattgct tccatttcct gcatgatatc ggttgcgcgg tttttgctca
      181 tattgcggaa aaacttgaaa cgaatttcat cacctgctcc tttcagggcc tttgcaatca
      241 tatagtcatt attgatttca tcaataagta tccgcatctc ggtattggtg agggtaagaa
      301 cattttcaaa ggtaaagatg cgactcctta tttcatcagc aatttgcggc atagtggttt
      361 caaagtattc aattagctgt ttttcctgat cgccactcaa gtgcgccatg atatcaataa
      421 gcgcattgag tcctcccgct ttagaactgg attgcccctg gtagcgatac tcatcatatt
      481 ttttgcgcaa cacccgtgat atttcaagca ccgcatcagg cgatgttcgt tccatcttag
      541 ccatgcgcag cgtcacttct tttgccaaat ctttaggaag cagtttaata acttctgctg
      601 cttttgcggc agggatgtac gccagtgtta cggcaatagt ttggggatgt tcattctgca
      661 aaaatgtaac aattaactca gcatctatat cattaagaaa tccaaatcct ttttctaaat
      721 ccttatgggt tagccttttt aaaatgtcct ttgctttatc ttcaccaaac gctgcataca
      781 gtatttcttt ggcaacattc tgaccaccaa atgcaacccc acgcgctttt cgcaattcaa
      841 gtaaaaattg tcctatgagc tcctcttttt cttcaggtga tagtacatct atctttgcta
      901 tttctgcagc taatttattg acactttcct catcaagaaa tttcattact tctgaggcaa
      961 cctcaggacc aagtgccact aaaagcgctg ctgccttttc ggcaccgttc atctggtcaa
     1021 ttggcttcac gtttgattac cataattttt ttaaagtcta taaccttctg cggacatacc
     1081 tcagcacatc ttccgcaatt aatacataat tcataatcaa ttcccgctaa aaagttttca
     1141 actttgatag ccgtttctat atcaggattg tctttaaata cttcttcacg gcacgctttt
     1201 tcacatctct tgcaggctgt acagccaaca ctgcacccca acttcattac aggcgctttc
     1261 tgcttattgc gacagattac atagacatca actgtatttt caagcaggct aattatatca
     1321 cggggacagg cttcaacgca gttaccacat cctgtgcatt tttcagcatc aacaaacggt
     1381 agccccctat cgtccaaatg aatggcatca aaagggcatg cccgtacgca atcacccaat
     1441 cccaagcacc cgtattcgca caccttgaag ccacccataa caagctgagc agctctgcaa
     1501 ctatgtggtc catcgtacat gaaccgtatc ttggtttcat tcacaccacc ctggcagtgt
     1561 acgcgagcaa tgcgtttaac accagcctca gcttctatgc ccataatttc tgaaattttt
     1621 tttacgcttt ctgggccacc aactgggcac atatttattg ctgcaccatg ttcaacaata
     1681 ttggttgcat accccgcaca accgggaaac ccgcaagcac cacaatttgc accgggaagg
     1741 gcttctatta ctttctccac tcgggggtct ttttcaactt tcaataaaac cttagctagt
     1801 gatagcaaaa taccacatgc taagcttaat acagttatac tgattataga cggcactgaa
     1861 ggaaaaagta tatccagcat agttaacctc agcatttcat tttaagatat cattgtgtat
     1921 tacactatat aaaaagtcaa tactgttgca attaaaaaat tcttgcaata ataccattat
     1981 accgatagta tatgtaaaat caatgaataa taaccattta atctatggcg ccaacaacat
     2041 cacaggatat tttacacgag atactatcgg caataaataa aaacca
//
LOCUS       DAORYL010000205         2050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_36786_length_2050_cov_13.838596, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000205 DAORYL010000000
VERSION     DAORYL010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36786_length_2050_cov_13.838596"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            710..1585
                     /locus_tag="PLV81_15765"
     CDS             710..1585
                     /locus_tag="PLV81_15765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOM11598.1"
                     /translation="MHVNIYTLEEIYKTYENIEDHISTRQIIRRFSENKQDIRLLTLN
                     ECKLTHCKHILDLGCGYGFFTEALSNKISPDAHITGIDVIGGENKKMFLSTCAQSGYA
                     ATFIEDTANIITSFPDESYDCVIASYSLYFFPHLIEHIARILKTNGIFIALTHSEYTL
                     EQITTLFMESIHHILQLGDSSLHIIKLFKAFSAENGFFKLQPYFDYITYIRYPNNLIF
                     KPHDIDHCISYIQKKQYLFFKDIASLQAEEYNALIDDFYNRLRWYATSYNKFVVNKND
                     AIFIARKLKNKDAAS"
     gene            1582..>2050
                     /locus_tag="PLV81_15770"
     CDS             1582..>2050
                     /locus_tag="PLV81_15770"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOM11599.1"
                     /translation="MKKKRIFCSYCGAPITVKFIDEKYRDHCDNCNTTFYENPLPVAS
                     CIVINDKREVLLVQRKNDPYKNMWCLPIGFAETGESIEQAALRELKEEAGVTGEIVRI
                     IDVDTVSNYFYGDLAIITFEVKQLSPTIKAGDDALDAKFFPLSNYPPLAWESNE"
BASE COUNT          661 a          414 c          338 g          637 t
ORIGIN      
        1 tgcgattggt atatcattcc acattaaggc aaaaaaaatc ccctattagc ggggttgtgc
       61 atcattgacg tgtaggggct gatttcatat ccgccccaat cccctattag cggggcagtt
      121 ttcggacgca aaaaacttat ttaaacattg ttcatattac gatgaacgag tcgcaatccc
      181 ctattagcgg ggcagttttc ggacactctt ggggttgtaa ctcctggaaa ttccagagtt
      241 ttcaagccca tttccgccaa cctcttgtac aaacataaaa attatgttgg ctctgacaat
      301 acaacatttg taaagcctgg aatttccagt gttttcgttt ccgccaacct cccccaaaaa
      361 tggccgtttt atgtcacttc cttaggttgg cggcatcaaa atatgcctta ttttatacag
      421 ttttcaaaga actgtctaat aacagctctc tatcgcttgt aactttttat atctttgtaa
      481 catttctgtc aactattttt ttattctatt gccttccatt ttctgcagca aaattactgg
      541 tgatatttct aatgcatttt actggtaaaa ctgttaaatc ttttaccgcc tgtaacgtat
      601 tgaacagtaa ctatcgccca aaaatcataa gcctttacta aattaataca ttgacacttt
      661 atttttttta atgtataatt tttgaagcag tttcaattgt gataccgcca tgcatgtaaa
      721 catttataca ttagaagaaa tatacaaaac atacgaaaac attgaagacc atatttcaac
      781 caggcaaata atacggcgtt tttctgaaaa caaacaggat attcgcttac tcacgctaaa
      841 cgagtgtaaa ctgacacact gtaaacatat tcttgattta ggatgcggat atggattttt
      901 tacggaagcg ctgagtaata aaatttcacc tgatgcacat ataactggca ttgatgtcat
      961 tggtggtgaa aacaaaaaga tgtttctttc aacctgtgcg caaagcggct atgcggcaac
     1021 atttattgaa gacactgcaa acatcataac atcattccct gatgaatcgt atgactgtgt
     1081 tattgcaagc tattcactgt atttcttccc tcaccttatt gaacatattg cccgcatatt
     1141 gaaaacaaat ggcatcttta ttgcccttac ccacagcgaa tatacacttg aacaaatcac
     1201 aacgcttttt atggaaagta ttcaccatat attacaattg ggcgatagtt ccttacatat
     1261 aatcaaactg tttaaagcat tctctgctga aaatggattt tttaaattac aaccgtactt
     1321 tgattatatc acgtatatac gatatccaaa taatctcata ttcaaaccgc atgatattga
     1381 ccactgtata agttatattc agaaaaaaca gtatctcttt tttaaagata ttgcttcact
     1441 gcaagcagaa gaatataatg ctctcataga tgacttttat aaccgcctgc gatggtatgc
     1501 aacctcatat aataagtttg ttgttaataa aaatgatgca atttttattg cccgcaagct
     1561 caaaaacaag gatgcagcat catgaagaaa aagcgtattt tctgcagcta ttgtggtgca
     1621 ccaataacag taaaatttat tgatgaaaaa taccgtgacc actgtgacaa ctgcaacact
     1681 acattctacg aaaacccact tcctgtagca tcgtgtattg ttataaatga caaacgagaa
     1741 gtgttactgg ttcaacgaaa aaatgacccc tacaaaaaca tgtggtgtct tcccattggc
     1801 tttgcagaaa cgggcgaatc aattgaacag gcagcattgc gcgaattgaa agaagaagca
     1861 ggtgttaccg gtgaaattgt acgcatcata gatgtggata cagtatcaaa ttacttttat
     1921 ggcgatttag caattatcac atttgaagtc aagcaactat cgccaaccat aaaagccggc
     1981 gatgatgcac tggatgcaaa gtttttccca ctatccaatt acccaccact tgcctgggaa
     2041 tcaaatgaaa
//
LOCUS       DAORYL010000206         2041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_37021_length_2041_cov_6.894763, whole genome shotgun sequence.
ACCESSION   DAORYL010000206 DAORYL010000000
VERSION     DAORYL010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37021_length_2041_cov_6.894763"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1199)
                     /gene="metK"
                     /locus_tag="PLV81_15775"
     CDS             complement(48..1199)
                     /gene="metK"
                     /locus_tag="PLV81_15775"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011733999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOM11600.1"
                     /translation="MGRNYLFTSESVSEGHPDKVADQISDAILDEHIKGDPYSRVACE
                     TLVTTNRVVISGEITSAVTVDYEAIARKVIAEIGYTSPEIGFDAKNCIVEVFIHPQSP
                     DISQGVTEGQGLFKEQGAGDQGMMFGYAVAETDELMPMPILYAHRLVKRLSEIRKKGE
                     VNFLRPDGKSQVTVEYENDKPKRISAIVISTQHDDAVTLPTLEEMVRELVIKKVIPPH
                     LWDSNTTIYVNPTGRFEVGGPHGDTGLTGRKIIVDSYGGMGRHGGGAFSGKDPSKVDR
                     SAAYMGRYIAKNIVAAGLAYRCELQVAYAIGVADPVSVMVDTFGTNTIPEEEIEKRIK
                     AIFNLKPKSIIQTLDLLKPKYKATAAYGHFGRNESGFTWEDTKVAAQLR"
     gene            complement(1325..1516)
                     /locus_tag="PLV81_15780"
     CDS             complement(1325..1516)
                     /locus_tag="PLV81_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOM11601.1"
                     /translation="MAKEVYVDQSECTGCELCVDTLPEVFEMTPDGVSKVKNSKGASE
                     DKIQEVIDSCPAECIHWKD"
     gene            complement(1579..1914)
                     /gene="secG"
                     /locus_tag="PLV81_15785"
     CDS             complement(1579..1914)
                     /gene="secG"
                     /locus_tag="PLV81_15785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOM11602.1"
                     /translation="MSILISIGTVLFVILSILLIIIILLQSDKSAGMGILGGSSNTAF
                     GSSTADIITKITTVMVALFMLGSLGLSVVESYRARSLEKDLLSEEKKGGVIEQKALEK
                     PQDKPQQPK"
BASE COUNT          573 a          475 c          343 g          650 t
ORIGIN      
        1 ctgcattcct tttcatactc ttcaaagttt taaaattaaa ttcttcttta ccgcaactgg
       61 gctgccacct ttgtgtcttc ccaggtaaac cctgattcat tacgaccaaa atgcccgtat
      121 gctgctgttg ctttatactt tggtttaagc agatcaaggg tttgaataat agattttggt
      181 tttaaattaa atattgcttt tattcgtttt tcaatttctt cttccgggat ggtattagtt
      241 ccaaacgtat caaccatcac tgatactgga tccgcaacac caatagcgta ggccacctgc
      301 aattcacatc ggtatgcaag ccctgcagcc acaatattct tggcaatata gcgtcccata
      361 tatgctgcag atcggtctac ctttgatgga tccttgcctg aaaatgctcc accaccatgt
      421 ctgcccattc caccgtaact atcgacaata atcttccttc ccgttaaacc ggtatcccca
      481 tgaggtccac ctacttcaaa gcggcctgtt gggttaacat atattgtagt attgctatcc
      541 cacaaatgtg gaggaattac cttttttatc acaagctcac ggaccatttc ttccagtgtg
      601 ggcaacgtga ctgcatcatc atgctgtgtt gaaatgacta ttgcacttat gcgtttgggt
      661 ttatcatttt catattctac agtaacctga gattttccgt cggggcgcag gaagttcacc
      721 tcaccttttt tgcggatctc agataaccgt ttgacaaggc ggtgtgcata taaaatgggc
      781 atgggcatca attcatcggt ttcagcaacc gcatacccaa acatcattcc ctgatcacca
      841 gcaccctgtt ccttaaaaag tccctggcct tcagtaacac cctgcgaaat atcaggggat
      901 tgtggatgta taaatacttc tactatacaa ttttttgcat caaacccaat ttcaggtgac
      961 gtatagccaa tttcagcaat aaccttgcgt gctattgcct cataatcaac agtaaccgct
     1021 gaggtaatct cgcctgaaat aacaacacgg ttggtggtaa ccagcgtttc acaggcaaca
     1081 cgtgagtatg gatcaccttt tatgtgttca tctaaaatgg catcagaaat ctgatcagca
     1141 accttatcag gatgcccttc cgaaacagat tcagatgtga ataaataatt tcttcccatt
     1201 gttatgttta ctcctttgcg cttacaataa ttgcccaacc cgcacttcta taaacattga
     1261 aaggcatatt gcatgaatat gccttgtaag tatggttaga atttaatata tatatagtga
     1321 tctatcagtc tttccagtga atacactcag ctggacagct atcaataact tcctgaattt
     1381 tatcctctga tgcacctttt gaatttttaa ctttacttac accatcaggt gtcatttcaa
     1441 aaacttctgg caatgtatca acacaaagtt cgcatcctgt acattcgctc tgatcaacat
     1501 acacttcttt tgccataggt tcctccattg gtttaagata tttatagcat tatactacaa
     1561 aggatatcca tttgcaattt attttggctg ctggggctta tcttgaggtt tttctaaagc
     1621 cttttgctca ataacaccac ctttcttttc ttcactcaaa aggtcttttt ccaggctgcg
     1681 cgccctgtat gactctacaa ctgaaagccc aagcgatcct agcataaaaa gggcaaccat
     1741 aacagtggta attttggtga taatatcagc agttgaagaa ccaaatgctg tgttacttga
     1801 cccacctaaa atacccattc cggcgctttt atccgactgc agcaggatga tgataatgag
     1861 cagtattgat aatattacaa acaatactgt ccctattgaa ataagtatgc tcatactgca
     1921 cctttatata ttcaataaat tttaaaaatg ctattatttg tgccttgtta caacgcaacc
     1981 tgtataatgt caagaaaaga ttgtacttta aggcttgcac caccaaccag cacaccgtct
     2041 a
//
LOCUS       DAORYL010000207         2035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_37193_length_2035_cov_9.031818, whole genome shotgun sequence.
ACCESSION   DAORYL010000207 DAORYL010000000
VERSION     DAORYL010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37193_length_2035_cov_9.031818"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1199)
                     /gene="metK"
                     /locus_tag="PLV81_15790"
     CDS             complement(48..1199)
                     /gene="metK"
                     /locus_tag="PLV81_15790"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011733999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOM11603.1"
                     /translation="MRRNYLFTSESVSEGHPDKVADQISDAILDEHIKGDPYSRVACE
                     TLVTTNRVVISGEITSAVTVDYEAIARKVIAEIGYTNPEVGFDASSCIVEVFVHPQSP
                     DISQGVTEGQGLFTEQGAGDQGMMFGYAVAETDELMPMPILYAHRLVKRLAEIRKKGE
                     VNFLRPDGKSQVTVEYENDKPKRISAIVISTQHDGAVTLPTLEEMVRELVIKKVIPAH
                     LWDSNTKIYVNPTGRFEIGGPHGDTGLTGRKIIVDSYGGMGRHGGGAFSGKDPSKVDR
                     SAAYMGRYIAKNIVAAGLAYRCELQVAYAIGVADPVSVMVDTFGTNTIPEEEIEKRIK
                     AVFNLKPKSIIQTLDLLKPKYKATAAYGHFGRNEPSFTWEDTKVAAQLR"
     gene            complement(1319..1510)
                     /locus_tag="PLV81_15795"
     CDS             complement(1319..1510)
                     /locus_tag="PLV81_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOM11604.1"
                     /translation="MAKEVYVDQAECTGCELCVDTLPEVFEMTPDGVSKVKNPQGASE
                     DKIQEVIDSCPAECIHWKD"
     gene            complement(1574..1909)
                     /gene="secG"
                     /locus_tag="PLV81_15800"
     CDS             complement(1574..1909)
                     /gene="secG"
                     /locus_tag="PLV81_15800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOM11605.1"
                     /translation="MSILISIGTVLFVILSILLVIIILLQSDKSAGMGILGGSSNTAF
                     GSSTADIITKITTVMVALFMLGSLGLSVLESYRARSLEKDLLSEEKSDGVIEQKALEK
                     PQEQPQQPK"
BASE COUNT          566 a          474 c          354 g          641 t
ORIGIN      
        1 ctgcattcct tttcatactc ttcaaagttt taaaattaaa ttcttcttta ccgcagctgt
       61 gctgccacct ttgtatcttc ccaggtaaaa ctgggttcat tgcggccaaa gtgtccgtat
      121 gctgctgttg ctttgtattt tggtttaagc agatcaagcg tttgaataat agattttggt
      181 tttaaattaa aaactgcttt tattcttttt tcaatttctt cttctggaat ggtattagtt
      241 ccaaatgtat ccaccatcac cgatacggga tcagccacac ctatggcata ggccacctgt
      301 agttcacatc ggtacgcaag acccgcagct acaatgttct ttgcaatgta tcggcccatg
      361 tatgctgctg agcggtccac ttttgatgga tctttccctg aaaatgcgcc gccaccgtgt
      421 cggcccattc caccataact atcgacaata atttttcttc ccgttaaacc cgtatcacca
      481 tggggtccac ctatctcaaa acggccagtg gggttaacat atatcttggt attgctgtcc
      541 cacagatgag cagggattac tttcttaatg acaagctcgc ggaccatttc ttctagtgtg
      601 ggtaacgtga ctgcaccatc atgctgtgtt gaaatgacaa tggcacttat gcgttttggt
      661 ttatcgtttt catactcaac ggtaacctgc gacttgccat cggggcgcaa aaaatttacc
      721 tcacctttct tgcggatttc agccaatctt ttcacaagac ggtgtgcata cagaattggc
      781 attggcatca gttcatcagt ttctgcaacc gcatacccaa acatcatacc ctgatcacca
      841 gcaccctgtt ccgtaaaaag accctgccct tcagtaacac cctgtgagat atcaggggat
      901 tgcggatgta caaagacttc cacaatacag cttgatgcat caaaacccac ttccgggtta
      961 gtgtagccaa tctctgctat aactttacgc gctattgcct catagtcaac agtaaccgct
     1021 gaagtaatct cacctgaaat aacaacacgg tttgtggtaa ccagcgtttc acaggcaaca
     1081 cgtgagtatg gatcaccttt tatgtgttca tctaaaatgg catcagaaat ctgatcagca
     1141 accttatcag gatgcccttc cgaaacagat tcagatgtaa ataaataatt tcttctcatt
     1201 gtaatatttt ctcctttgcg ctttcaatta ttgcccaacc catacttcta taaacataga
     1261 aaggcatatt gcatgaatat gccttgcgag taggtttaag atttatatat gtaatgtatc
     1321 agtctttcca gtggatacac tcagccggac agctatctat aacttcttga attttatcct
     1381 ctgatgcacc ttgtggattt ttaactttgc tcacaccatc aggtgtcatt tcaaaaactt
     1441 ctggcaatgt atcaacacaa agctcgcatc cagtacattc ggcctgatca acatacactt
     1501 cttttgccat agattcctcc agtggtttaa gataatttat agcattatac tacaaaagat
     1561 atccatacgc aatttatttt ggttgttggg gctgttcttg aggtttttct agagccttct
     1621 gctcaattac accatctgac ttttcttcac tcaaaagatc tttttctaag ctgcgggctc
     1681 tgtatgattc taaaactgaa agccccaatg aacctagcat aaaaagcgca accatgacgg
     1741 ttgtaatttt ggtgataata tcagcagttg aagaaccaaa ggctgtatta cttgaaccac
     1801 ctaaaatacc catccccgcg cttttatctg actgaagcag gatgatgata acaagcagta
     1861 ttgataatat aacaaacagt actgttccta ttgaaataag tatgctcata ctgcaccttt
     1921 atatagtaat aaaattttaa aatgctttta tttatgcctt gttacaacgc cacatttatt
     1981 atgtcaagaa aagattgtac tttaaggctt gcaccaccaa ccagcacacc gtcta
//
LOCUS       DAORYL010000208         1972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_38870_length_1972_cov_5.561294, whole genome shotgun sequence.
ACCESSION   DAORYL010000208 DAORYL010000000
VERSION     DAORYL010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38870_length_1972_cov_5.561294"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1550)
                     /locus_tag="PLV81_15805"
     CDS             complement(<1..1550)
                     /locus_tag="PLV81_15805"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOM11606.1"
                     /translation="MQVWYTRTYPLGRFGIPVAVQRLRSKIISIVIPITLLTSVFLLV
                     LFYRIDKSNVDSLLTARINDSFTSIQLDNNEIFTQSIPTVIKEYEGVILVTEDNTVVM
                     SNRDEFISLPINQTIKKGNQPQFLYTDTLKFIKNIKTMKYPKIEGVFDGNQAVLYGYN
                     VPQSNRTIIAAFNEKILYKTVYRSLFFIILGMIVVNLIIRYLINKQLMKLSKAIDIAM
                     PALQKATKGDLSGEITLIKSRDIMEDFIRSFIGFREVIIEFIQKSNELSEKVLISSEA
                     LAFSGKSIKEEASQQAQNIEQVTSIIAEVSSSFYTIASDANKQSDILKNLEDIVNNLN
                     AAMNILNNDAQQVIVAIRGVEKSAHDSENLVTSTAESSRQIASFFENIMNVIGLISDI
                     AEQVNLLSLNASIEAARAGEHGRGFAVVAEEISRLADRTAQNVKEISKIINEGNTAMN
                     SNMSMMDTMRKVLMNIMQDIERSVTLITGFIETINKRVQDFAKIHEGIASASEFSKGL
                     SLSMAELS"
BASE COUNT          651 a          340 c          274 g          707 t
ORIGIN      
        1 tcactcaatt cagccattga tagcgatagt ccttttgaaa actcactggc actggcaata
       61 ccttcatgaa tctttgcaaa gtcctgaact cttttattta ttgtttcaat aaatccagtt
      121 atcaatgtta cactgcgttc aatatcctgc ataatgttca tcaatacttt acgcatggtg
      181 tccatcatgc tcatgttgct attcattgcc gtgttgcctt cattaattat tttagaaatt
      241 tccttaacat tttgtgctgt cctgtctgca agtcttgaaa tttcttctgc aaccactgca
      301 aatcctctgc catgttcacc agcccttgct gcctcaattg atgcattgag tgacaaaagg
      361 ttaacctgtt cagcaatatc agatattaaa cctataacat tcataatatt ttcaaagaaa
      421 gaagctattt gcctggatga ttctgccgta ctagttacaa gattttcact gtcatgagcg
      481 cttttttcaa ctcctcgtat tgcgacaatg acctgctgtg catcgttatt taatatattc
      541 attgcggcat taagattatt gacaatatct tcaagatttt tcaggatatc actttgctta
      601 ttggcatcag aagcaattgt atagaaggaa ctggatactt cggctataat tgaagtaacc
      661 tgttcaatat tttgtgcctg ttgtgaagcc tcttctttta ttgattttcc agagaatgct
      721 aaagcttcgg atgaaatgag taccttctcg ctgagttcgt ttgatttttg tataaattca
      781 ataattactt ccctgaaacc aataaaactt cgtataaaat cttccatgat gtcacgggat
      841 tttattaatg taatttcccc tgacagatcg cctttggttg ccttttgcaa agcaggcata
      901 gctatatcga tagcttttga aagtttcatc agttgtttat taataagata tctgataatt
      961 aaatttacaa ctatcatccc aagtattata aaaaataaac tcctgtatac agttttatac
     1021 aatatctttt cattaaaggc agcaattata gtacgattgc tctgaggcac attataacca
     1081 tataacacag cttgattgcc atcaaataca ccttctattt ttggatactt cattgttttt
     1141 atatttttta taaatttcag agtatcagta tataaaaatt gtggctggtt tcctttttta
     1201 attgtttgat taataggtag tgatatgaat tcatctctat tactcataac aacagtatta
     1261 tcttctgtaa ctagaattac accttcatat tctttaatca ccgtaggtat actttgagta
     1321 aaaatttcat tattatctaa ttgaattgaa gtaaaactat cattaattct tgctgtaagt
     1381 aatgaatcaa cgtttgattt atctatacgg taaaataata caagaaggaa tactgatgtc
     1441 aatagagtta taggtatgac tatgcttata attttactac gaagtctttg aactgcaaca
     1501 ggtataccaa aacgtcccaa tggatatgtc ctggtatacc atacctgcat tgaattaaaa
     1561 cctatgaata agaacacaaa tactaaaata aagaaaacat aaaaatgata atataagttg
     1621 ttgtatccaa atttaaaata cattaagaaa aacaaaggga tatatacaat aattaaatat
     1681 cccatattaa gtaacattgt ttgctgtgca cttgtcttaa atttattaaa tatcttttca
     1741 tacctttgtg ggtcatcact ttcaaataat cgtagtatac ttttaaccat ccacatataa
     1801 taagtgaata atgatgaaaa agatataacc cataacataa taaatgatac taacaacctg
     1861 atatctttaa ataatgggaa agacgtactc ataattatta caaatagtaa tgcattaaat
     1921 aaaataacac cactaccata cttctggaat tgtcttataa gctgttttgg tt
//
LOCUS       DAORYL010000209         1972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_38872_length_1972_cov_4.496609, whole genome shotgun sequence.
ACCESSION   DAORYL010000209 DAORYL010000000
VERSION     DAORYL010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38872_length_1972_cov_4.496609"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1550)
                     /locus_tag="PLV81_15810"
     CDS             complement(<1..1550)
                     /locus_tag="PLV81_15810"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOM11607.1"
                     /translation="MQVWYTRTYPLGRFGIPVAVQRLRSKIISIVIPITLLTSVFLLV
                     LFYRIDKSNVDSLLSDRINDSLITLQLDTNETFLKSIPAVIKEYEGVILVSEDNIVIM
                     SNRNAFISLPLTQTIKKGNQPQFLYNDTIQFLQNIKSTKYSKADGIFDGNHAVLFGYN
                     VPQSNRCIIFAFNEKVLYRTVYRSLFFIILGMIVINLIIRYLINRQLMKLSKAIDIAM
                     PALQKATKGDLSGEITLIKSRDIMEDFIRSFIGFREVIIEFIQKSNELSEKVLVSSEA
                     LAFSGKSIKEEASQQAQNIDQVTSIIAELSSSFYTIASDASKQSSILKNLEDIVNNLN
                     TAMNVLNNEAQQVIVAIKDVEKSARDSETLVSSTAESSKQIASFFENIMNVIGLISDI
                     AEQVNLLSLNASIEAARAGEHGRGFAVVAEEISRLADRTSQNLKEISKIINEGNTAMN
                     SNMSMMDTMRKVLMNIMQDIERSVTLITGFMETINQRVQDFAKIHEGITSASEFSKGL
                     SLSMAELS"
BASE COUNT          657 a          334 c          285 g          696 t
ORIGIN      
        1 tcactcaatt cagccattga tagcgatagt ccttttgaaa actcactggc actggtaata
       61 ccttcatgaa tctttgcaaa gtcctgaacc ctttgattaa ttgtttccat aaaacctgtt
      121 attaatgtta cactgcgttc aatatcctgc ataatgttca tcaatacttt ccgcatggtg
      181 tccatcatgc tcatgttgct attcattgct gtgttgcctt cattaattat tttagaaatt
      241 tccttaaggt tttgggaagt tctgtctgca agacgtgaaa tttcttcagc aacaactgca
      301 aatcctctgc catgttcccc agcccttgct gcctcaattg atgcattgag tgacaaaaga
      361 tttacctgtt cagcaatatc agaaattaaa ccaatgacgt tcattatatt ttcaaagaaa
      421 gaagctattt gtttagaaga ttctgcagta ctggaaacca gtgtttcgct atctctggca
      481 cttttttcaa catcttttat agcaactata acttgttgcg cttcattatt caatacattc
      541 attgcagtat taagattatt aacaatatct tcaagatttt ttaagatact gctttgttta
      601 ctagcatcgg atgcaattgt gtaaaaggaa ctggataatt cggctataat tgaagtaacc
      661 tgatcaatat tttgcgcctg ttgtgaagct tcttctttta ttgattttcc tgagaatgct
      721 aatgcctcgg atgaaacaag cactttttcg ctgagttcat ttgatttttg tataaattca
      781 ataataactt ctctgaaacc aataaaactc cgtataaaat cttccatgat atcacgggat
      841 tttattaaag ttatttcacc cgacagatca cctttggttg ccttttgcaa ggcgggcata
      901 gctatatcga tagcttttga aagtttcatc agttgtctgt taataagata tcttatgatt
      961 aagttaatga ctatcattcc aagtataata aaaaacaaac ttctatagac agttctatat
     1021 aatacctttt cattaaatgc aaatattata caacgattac tttgaggcac attataccca
     1081 aataatactg catgattacc atcaaatatg ccgtcagctt ttgagtactt agttgatttt
     1141 atattttgca aaaattgtat ggtatcatta tataaaaatt gtggttgatt gcctttttta
     1201 attgtttgtg taagcggtaa tgatataaat gcattcctat tgctcataat tacaatatta
     1261 tcttctgaaa caagaataac accttcatat tctttaatta ccgctggtat gctttttaaa
     1321 aatgtttcat tagtatccaa ttgtaatgta attaaactat catttattct gtctgaaagt
     1381 aatgaatcaa catttgattt atctatacgg taaaataata ccagaaggaa tactgatgtc
     1441 aaaagagtta taggtataac tatgctgata attttactcc taagcctttg aacggcaacc
     1501 ggtataccaa aacgtcccaa tggatatgtc ctggtgtacc atacctgcat tgaattgaaa
     1561 cctatgaata agaacacaaa tactaaaata aagaaaacat aaaaatgata atataagttg
     1621 ttgtatccaa atttaaaata cattaagaaa aacaaaggga tatatacaat aattagatat
     1681 ccaatattta ataacattgt ttgctgtgca ctcgtcttga atttattaaa gattttttca
     1741 tatctttgcg ggtcatcact ttcaaacaat cgtagtatac ttttaaccat ccacatgtaa
     1801 taagtgaata atgatgaaaa agatattacc catagaataa caaatgatag taacagccgt
     1861 atatctttaa ataatgggaa tgatgtacca ataataataa cgaatagcaa tgcattgaat
     1921 aaaataacac cactaccata cttctggaat tgtcttataa gctgttttgg tt
//
LOCUS       DAORYL010000210         1936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_39824_length_1936_cov_12.129187, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000210 DAORYL010000000
VERSION     DAORYL010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39824_length_1936_cov_12.129187"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..1583
                     /locus_tag="PLV81_15815"
     CDS             66..1583
                     /locus_tag="PLV81_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOM11608.1"
                     /translation="MLSQALSLVIHGIDAHLIDVEVDIVKGLPNFTIVGLPDSVIRES
                     KDRIRSAIENSGFEFPPKNFIVNLAPAGYRKEGANLDLPIAVSILIATGQVESARVDI
                     PMVGELSLDGRVRPIKGVTSMAIALYRAGIYEFIVPYENRNEATAIEGIKVYPVKDIA
                     QAVEACCGKIQAYTHHESGDSSSNGYLDMASVMGQESAKRALEIAAAGRHNILLYGPP
                     GSGKTMLAKCVPGILPPLTKEQAITTTMIHSVGGTLGHGQGLIYTPPFRAPHHTSSDA
                     ALVGGGKIPTVGEVSLAHNGVLFLDEFVEFRNDVIQALRQPLEDCHITISRAMGTMTF
                     PADFMLVASSNPCQCGYLFDPEIPCKCSPGKIRSYFSKIAGPILDRIDIEVLVGRVPY
                     KDLLGKGNAESSEVVRQRVIKARDIQAWRFKGRTTTSNGRMTSDEVKEFCAINAECES
                     ILELAIKRMNLSARSFFRILKVSRTIADLDNSETIEKRHILEALSYKNLQRYYDI"
BASE COUNT          569 a          397 c          429 g          541 t
ORIGIN      
        1 caaaattttt taaatttttt cttcctcatg cgttgtatat ataaaacacg tacggaggaa
       61 tacttatgct ttcacaggct ttatcgcttg tcattcatgg cattgatgcg caccttattg
      121 atgtggaggt ggacattgtt aaagggctgc caaactttac cattgttggt ttgcctgatt
      181 ccgtaatccg cgaatcaaaa gacaggattc gctcggcaat tgaaaatagt ggttttgaat
      241 tcccaccaaa aaattttatt gtcaacttag cccctgctgg ctatcgcaag gaaggtgcta
      301 acctggattt acccattgca gtttcaatac ttatagcaac agggcaggtg gaaagtgcac
      361 gtgttgatat acccatggtt ggggagctat ccctggatgg aagagtgcgc cccatcaaag
      421 gagtcacatc aatggcaata gcgctatata gggctggcat ctatgagttc attgtcccgt
      481 atgaaaatcg caacgaagca acagctatag aagggataaa ggtatatccc gtaaaagata
      541 ttgcacaggc agtagaagcc tgttgtggga aaatacaggc ctatactcat catgaaagtg
      601 gcgatagttc cagcaacggg taccttgata tggcaagcgt tatggggcag gaatcggcca
      661 aacgggcact ggagatagca gctgcaggca gacacaatat attactgtat gggcctcctg
      721 gctcggggaa gaccatgctt gcaaaatgtg tcccggggat tttgccgccg cttactaaag
      781 agcaggcaat cacaaccaca atgatacatt cggttggtgg tacacttgga catgggcagg
      841 gacttatata cacaccgcca ttcagggcgc cacaccatac cagttctgat gcggcacttg
      901 ttggcggcgg caaaattccc accgtgggtg aggtatcact ggcacataat ggtgtgctgt
      961 ttttggatga atttgtggaa ttccgaaacg atgtgataca ggcactgcgt caaccactgg
     1021 aggattgcca tattaccata tcacgagcaa tgggaaccat gacattccct gccgatttta
     1081 tgctggttgc atcaagcaat ccctgccagt gtgggtatct ttttgatcca gagatccctt
     1141 gcaagtgttc gccaggcaaa atccgtagct acttcagtaa aatagcaggt cccatacttg
     1201 accgtattga tattgaagtg ctggttggaa gagtgccata taaggatttg ctgggaaaag
     1261 gcaatgctga aagttctgag gtagtacgtc aacgtgtaat aaaggcgcgt gatattcaag
     1321 cctggagatt taaaggaagg acaaccacca gcaatggacg catgaccagc gacgaggtaa
     1381 aagaattttg tgcaatcaac gctgaatgcg aatcaatact ggaacttgca ataaagcgaa
     1441 tgaatctttc tgcacgaagc tttttcagaa tattaaaagt gagccgcacc attgctgatc
     1501 tggacaactc agaaacaatt gaaaaacgcc atatcttaga agcactgtca tataaaaatc
     1561 tacagcgcta ttacgatatt tagtaaaaag gcggttgctt ttttgaagat taaatgtatg
     1621 tatgaaaact aacgaataca tggaaggcaa ttgattccat gtaaaaaaag agcttatatt
     1681 tcaacattga ctaacaaaaa gcaaggttct ttgcatattt tttatgtttt gatctccttt
     1741 ttgtgctttt cataacctct aaagtatgtt atggaatatt tcttacgata atattcctca
     1801 tactgcttta attttgccac attatggctc tgaccccatt tctatttttt ttacacccaa
     1861 tccactacat acgaatattt ttttcggtaa tactcctcat accgcataaa ctttgctacc
     1921 ctctcagcaa acgttt
//
LOCUS       DAORYL010000211         1926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_40109_length_1926_cov_10.645644, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000211 DAORYL010000000
VERSION     DAORYL010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40109_length_1926_cov_10.645644"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..1926
                     /locus_tag="PLV81_15820"
     CDS             64..1926
                     /locus_tag="PLV81_15820"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HOM11609.1"
                     /translation="MDLIDLIKKLEYTDKNIAAIEVHSYLKHLNKPVFYAKNYNKDLP
                     DKFAPVTGKEFLTIQYNIASALLDYGVEKGSVIGIFSRNNLYYAATVFAAMSLGAIVI
                     PIYPTILQDELEDILRDSGCRILFVGDVAQFQKGFAILNKIQSPLRRLITYFDSRTKH
                     EQLMTIDELLKFGEIKSNYEQINTIIPNINPDDNATLIYTPGTTGEPRGALLTHGNLL
                     SQKVVIPLFNFTDKDIRLAHLPFSHVYGFSADLLTSAYTGSPMAISTTFESEEIYRNM
                     IEIKPNVIATVPRMYEKLYISTIHTIRRFNPLKKFIYITAIHVGRECYVAETSGNIIP
                     KGADIINKILSPIKRSIRKMMHMQQVRLLFSGGAPLPLDVAYFFGGIGLPIIEGYGLT
                     ETSPVVTINRIEKNKPGTVGCPIPGAEIKISDEGEILIKGPMVFKGYNKMTPEEKAEI
                     FTPDGYFRSGDIGIIDEDGYLRITGRLKDIIITSQGKNIVPLNIEKKFESEPLINYIC
                     VVGDRRKYLSALIVPNFHLLRQYAREHGISYKTDEELVSHPQVQTLYKTRINEICQAL
                     PPYEQIKKFTLISHDFSVKSGELTPTFKFRRSQIHEKYKDIIDLMYPKSDITRL"
BASE COUNT          676 a          338 c          334 g          578 t
ORIGIN      
        1 taaactataa tttcaaagaa tgcattacag tgctgtgata taatgcatcg tatggggtgt
       61 actatggatt taatagactt aattaaaaag ttagaatata cagataaaaa tatagcagca
      121 attgaagtcc attcttattt aaaacatctc aataaacctg tattttatgc aaaaaattat
      181 aataaagact tgcctgataa atttgcacct gtaaccggaa aagaattttt aactatccag
      241 tataacattg cttctgcatt gttagattat ggtgttgaaa aagggagtgt cataggaata
      301 ttcagtagaa acaacctgta ttatgctgca accgtttttg ctgcaatgtc tttaggtgcg
      361 atagtaatac caatttatcc aactatttta caggatgaac tggaagatat attacgtgat
      421 tccggatgca ggatcctctt tgttggtgat gtagctcagt ttcagaaagg atttgcaata
      481 ttaaataaaa tacaatctcc tctacgaaga ttaatcactt attttgattc acgtaccaaa
      541 catgaacagc ttatgactat tgatgaactt ttaaaatttg gtgaaataaa atccaactac
      601 gaacaaatta atacaataat tcctaatata aatcctgacg acaacgcaac ccttatctat
      661 acacctggta ctacaggaga gccaagaggg gcattactaa cccacggcaa ccttctttca
      721 cagaaagttg ttatcccttt gtttaatttc actgataaag atattcgatt agcgcatctg
      781 ccttttagcc atgtatatgg cttttcagca gaccttttaa ccagtgccta tactggatct
      841 ccaatggcaa tatcaacaac ctttgaaagt gaagaaatat accgcaacat gatagaaata
      901 aaaccaaacg tcattgcaac agttccccga atgtatgaaa aattatatat tagtactatt
      961 cacaccatcc ggcggtttaa cccactaaag aaattcattt atataacagc catacatgtt
     1021 ggtagggagt gctatgttgc agaaacttct ggtaatataa ttcctaaggg tgctgacata
     1081 ataaacaaga tactatcgcc cattaaacga agtatccgta aaatgatgca catgcaacag
     1141 gtaagactcc ttttttctgg gggtgcacca ttaccactgg atgtagctta cttttttggt
     1201 ggcattggat taccaataat tgaaggttat ggcttgaccg agacatcacc cgttgttaca
     1261 ataaatcgta ttgaaaaaaa taaacctggt accgttggct gcccaattcc tggtgcagag
     1321 attaaaattt ctgatgaggg tgagattctt attaaaggtc ccatggtttt taagggttac
     1381 aataaaatga ctccagaaga aaaagcagaa atttttacac ctgatggcta tttcagatca
     1441 ggcgacattg gtattataga tgaagatggc tatttacgta taaccggtag gcttaaagat
     1501 atcattatta cttcacaggg caaaaatatt gtaccattga atattgaaaa gaaatttgaa
     1561 tcagaacctt tgataaatta tatctgtgtg gttggtgaca gacgaaaata cttatcagca
     1621 ctcattgttc ctaattttca tcttttgcgg caatatgccc gagaacatgg aatttcatac
     1681 aaaaccgacg aagaactggt atcccatcca caggttcaaa cattatataa aacaaggatt
     1741 aatgaaatct gtcaggcatt accaccatat gaacaaataa aaaaattcac actgatttcg
     1801 catgattttt cggtaaaaag cggagaactg actccaacgt ttaaattcag gcgaagccag
     1861 attcatgaaa aatataaaga tattatagac ctgatgtatc caaagagtga tatcacacga
     1921 ttgtaa
//
LOCUS       DAORYL010000212         1912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_40531_length_1912_cov_9.849219, whole genome shotgun sequence.
ACCESSION   DAORYL010000212 DAORYL010000000
VERSION     DAORYL010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40531_length_1912_cov_9.849219"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PLV81_15825"
     CDS             <1..257
                     /locus_tag="PLV81_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11610.1"
                     /translation="FFFLYFNHKQKMLLIEKGLYQKISFDFDSFSLFSGFLLTGVGGA
                     LTLFFVLKDGISYALIGGLVPLGLGISFIMYYFTKMKTTK"
     gene            254..811
                     /locus_tag="PLV81_15830"
     CDS             254..811
                     /locus_tag="PLV81_15830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOM11611.1"
                     /translation="MTAQHDEYIVQQILKGKQELFSIIVERYQNHIFTLGMRFFKNYD
                     DAADFTQEVFVKVYNNLASFKGLASFKSWLMKVGYNHAVNKLATIKNNNSDEYNDSID
                     NCPNPETQVMQNELQSILEKAISTLPQEYRICIDLYFYLGLPFKEISNITGYPVNTIK
                     SYVFRAKQQLRHALKGTIAEEYHEM"
     gene            801..1247
                     /locus_tag="PLV81_15835"
     CDS             801..1247
                     /locus_tag="PLV81_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11612.1"
                     /translation="MKCEKAIEKYLSLDNNKSMPLSLMIHLFACKQCRKEIEDLRSIF
                     TTLQNPPYAISLENKIMQQIMLQKSYYQKVSNFNWVAAGLIIVLSIGVISYSDTLQWL
                     SLHFGNKILVPLYLVMGCIVSGYIGSYVATHLKKLEAIAHSIKSLL"
     gene            complement(1244..>1912)
                     /locus_tag="PLV81_15840"
     CDS             complement(1244..>1912)
                     /locus_tag="PLV81_15840"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOM11613.1"
                     /translation="SQIELRLAAHLSGDPTMIEAFHKGIDIHAQTASAVFNVPLEKVT
                     DTMRRQAKIINFATIYGVSPYGLSQQADISFDDATQFIEKYFSAYPKIKDYIDSTIAF
                     ARKHGYVQTLLGRKRYIPEITSSNQFRREGAERIAINTPIQGTSADMIKVAMIAIHKA
                     FTDKKLKSRMILQVHDELVFEVPEDEKDIVEGIVVSAMKSAITLDVPVVVDVGWGKNW
                     AQAH"
BASE COUNT          595 a          394 c          342 g          581 t
ORIGIN      
        1 tcttcttttt cctgtatttc aatcataaac aaaagatgct tctcattgaa aaaggattgt
       61 atcagaaaat atcatttgat tttgattcat tcagcctttt cagcggattt ctactaaccg
      121 gagttggtgg tgcacttacc cttttctttg ttcttaaaga tggcatatca tatgctctta
      181 tagggggtct ggttccactg ggattgggca taagcttcat catgtattac tttaccaaaa
      241 tgaaaacaac aaaatgacag cacaacatga tgagtacatt gtacaacaga ttcttaaagg
      301 caagcaggaa ttattttcaa ttattgttga acggtaccag aatcacatat ttacactggg
      361 aatgcggttt tttaaaaatt acgatgatgc agctgacttt actcaggagg tttttgttaa
      421 agtgtacaat aatttagctt cattcaaagg gcttgcatca tttaaaagct ggcttatgaa
      481 agttgggtac aaccatgccg ttaacaagct tgccaccatt aaaaacaata attccgatga
      541 atataatgac agtattgata attgtcccaa cccggaaaca caggtaatgc agaatgaact
      601 acagtcaata cttgaaaaag ccattagcac tcttcctcaa gaataccgta tatgcattga
      661 cctgtatttt taccttggtt taccatttaa agaaatttct aacataaccg gttatcccgt
      721 aaatacaatc aagtcgtatg tcttcagggc aaagcagcaa ttacgacatg cattaaaagg
      781 caccatagcg gaggaatacc atgaaatgtg aaaaagccat tgaaaaatac ttaagcctgg
      841 acaacaataa atccatgcca ttatcattaa tgatccatct ttttgcgtgt aagcaatgca
      901 gaaaagaaat tgaagatctg cgctctatat ttacaacctt acaaaatcca ccgtatgcca
      961 tatcattaga aaacaaaatc atgcagcaga taatgttaca aaaatcctac tatcaaaagg
     1021 tttccaattt taactgggtt gctgccggcc tcattattgt actgagcatt ggggttatat
     1081 cttacagtga tacactgcaa tggttaagcc tgcactttgg caataaaatc ctggtgccat
     1141 tgtatcttgt catggggtgc atagtatcag ggtatattgg cagttacgtt gcaacacatc
     1201 taaaaaaatt agaagcgata gctcatagta ttaagtcatt gctctaatga gcctgtgccc
     1261 agttttttcc ccaacctaca tccacaacaa ccggcacatc aagagtgatt gcactcttca
     1321 ttgcactcac tacaatgcct tcaacgatat ccttttcatc ttcaggcacc tcaaacacca
     1381 gttcatcatg aacctgaaga atcattctac tttttaactt tttatcagta aatgctttgt
     1441 gaatggcaat cattgccacc tttatcatat cagctgatgt tccctgaatg ggagtgttga
     1501 ttgcgattcg ttctgcaccc tcgcgccgga actgattgct tgatgtaatt tcggggatgt
     1561 agcgctttcg acccagcaat gtttgcacat acccatgttt ccgggcaaaa gcaatagtac
     1621 tgtcaatgta gtctttaatc tttggatatg cactgaaata tttttcaata aattgtgttg
     1681 catcatcaaa tgaaatatca gcctgctgtg aaagcccata tggcgaaaca ccatagattg
     1741 tagcaaaatt gataattttt gcctgcctac gcattgtatc cgtaaccttt tccagtggca
     1801 cattaaacac tgccgaggct gtctgagcat gaatatctat ccccttatga aatgcctcaa
     1861 tcatggtggg gtcacctgaa aggtgggcag ctaaccgcaa ctcaatctgc ga
//
LOCUS       DAORYL010000213         1879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_41533_length_1879_cov_13.702303, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000213 DAORYL010000000
VERSION     DAORYL010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41533_length_1879_cov_13.702303"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLV81_15845"
     CDS             <1..206
                     /locus_tag="PLV81_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11614.1"
                     /translation="SASEFSKGLSLSMAELSDNTKKIVQSIENVNAVAGQFVALSENL
                     TNTSSELESMAAELKKVIEHFKI"
     gene            complement(251..1546)
                     /gene="purB"
                     /locus_tag="PLV81_15850"
     CDS             complement(251..1546)
                     /gene="purB"
                     /locus_tag="PLV81_15850"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOM11615.1"
                     /translation="MIDRYSRKQIASIWSLENKFKIWLDIEIAACEANAQLGVIPQED
                     LAIIKEKANFNVSRIEEIENQVHHDVIAFLTCVAEYVGPSSRYIHYGLTSSDIVDTAL
                     SIQMKQAGEIILNDIDQLLEVLKKLALKYKHTPCMGRSHGVHAEPTTFGTKMGLYYHE
                     FLRNRKRMQDAIDEISYGKLSGAVGTFSNIDPRVEEIVCKKLGLKPDPISTQIIQRDR
                     HAHYMSAVAICAGTLDKLATEIRHLQRTEVREVEEPFQKGQKGSSAMPHKRNPILSER
                     VSGLSRVIKANLMVALDNMTLWHERDISHSSAERVILPDSTIALDYLLSKMIFILENL
                     HVYPDAMMKTIEKTGGLFYSQGLLLKLVEKGYTREEAYQIVQDNAMRVWNAGGSLKEL
                     SLNDGRITAKLSHDEIEEIFKLENFLHNIDYVFKKAGLE"
     gene            1735..>1879
                     /locus_tag="PLV81_15855"
     CDS             1735..>1879
                     /locus_tag="PLV81_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012860042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HOM11616.1"
                     /translation="MEYLNQIRDFVWGTPLIILLLGTGIYLTILLKGLQFRTLFSSLY
                     LALI"
BASE COUNT          561 a          365 c          319 g          634 t
ORIGIN      
        1 ccagtgccag tgagttttca aaaggactat cgctatcaat ggctgaattg agtgataata
       61 ccaaaaagat agtccaatcc attgaaaacg ttaatgcagt tgctggccag tttgttgctc
      121 tatctgaaaa tctgacaaac acttcatcag aacttgaatc tatggctgca gagcttaaaa
      181 aagttattga gcattttaag atatgatgaa acatctactt ccgccgtttc agtctgaaat
      241 gtttgatgtt ttattctaaa cctgcttttt taaatacata atcaatatta tgtaagaaat
      301 tttccagttt gaatatttct tctatttcat cgtgcgaaag ttttgcagtt atccttccat
      361 cattgagtga tagttccttt aaagagcctc ctgcattcca cactctcatt gcattatcct
      421 gaactatctg gtatgcctct tcacgggtat agcccttttc taccagttta agtaataaac
      481 cctgtgaata aaacaaccca cctgtttttt caatagtttt catcatggca tcaggataca
      541 catgaaggtt ttcaaggata aatatcatct ttgaaagcag ataatcaagc gcaatagtgc
      601 tgtcaggaag tataacacgc tcggctgatg aatgtgatat atcacgttca tgccacaacg
      661 tcatattatc caacgcaacc ataaggtttg cctttattac ccgtgataac ccagataccc
      721 tctcggacaa gatgggattt cgtttatgag gcattgccga tgaccctttt tgacctttct
      781 gaaaaggttc ttcaacttct cgaacttcag tccgctgcag gtggcgtatt tcggttgcca
      841 atttgtccag tgttcctgcg caaatagcta cagctgacat ataatgtgcg tgtctatctc
      901 gttgaattat ttgcgttgat attggatcag gttttaggcc taatttttta caaactatct
      961 cttcaacacg cggatcaata ttgctgaacg tgccaacagc acctgaaagt tttccatatg
     1021 atatttcgtc gatagcatcc tgcattcgtt tacgattacg taaaaattca tgataataca
     1081 atcccatctt ggtgccaaat gtcgttggtt ctgcatgaac gccatgtgat cgccccatac
     1141 atggggtatg tttgtatttc agtgctaatt ttttcagtac ttccagaagc tggtcgatat
     1201 cattaagtat tatttcacca gcctgtttca tctgaattga gagtgcagta tcaactatat
     1261 cacttgatgt gaggccataa tgaatgtatc tgcttgatgg tcctacatat tcggcaacac
     1321 aggttaaaaa tgctatcaca tcatgatgta cctgattttc tatctcttca atgcgcgaaa
     1381 cattaaaatt agctttttct ttgattattg ctaaatcctc ttgtggtata acgcctaact
     1441 gtgcatttgc ttcgcaggcg gctatctcaa tatcaagcca tattttaaat ttattttcca
     1501 gtgaccatat actggcaatt tgtttgcgtg aatatctatc aatcatacaa acctcatact
     1561 tcaatatcaa tatatagtta ataataaata aaggaaaaga caaattttcc ttcaataagg
     1621 aaaataatca agtaaaaaag ataatttgga ttgttagtaa aattactgct tgacggatag
     1681 ggtaacaata tagcatcaat gtaaaacgat acaaattttt tatcaaggta tactatggaa
     1741 tatttaaatc aaatacgcga ttttgtgtgg ggaactccac tgatcatttt actgcttggt
     1801 acaggaatat atcttactat attgctcaaa ggattacaat tcaggacact gttttcttct
     1861 ttatacctgg cactaatta
//
LOCUS       DAORYL010000214         1863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_42079_length_1863_cov_6.013827, whole genome shotgun sequence.
ACCESSION   DAORYL010000214 DAORYL010000000
VERSION     DAORYL010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42079_length_1863_cov_6.013827"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PLV81_15860"
     CDS             <1..263
                     /locus_tag="PLV81_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOM11617.1"
                     /translation="TIDVINNLPVTRKELERMRERIERSSNYDSIADFFIENPDPAAE
                     GMGLGLSMVVVLLKSVGIPYTNFTLYTDFKTKTIARIIVPLM"
     gene            complement(299..931)
                     /locus_tag="PLV81_15865"
     CDS             complement(299..931)
                     /locus_tag="PLV81_15865"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOM11618.1"
                     /translation="MPRLKPEARAEKEEQRKTQIITAAYEAISAKGYNNFTLDDIAKN
                     AGLSKGGVLHYFKSKEDILIYLLEQIYIIIKKNINKRAAKYRTPERRMRAIVIAFIVT
                     AKRHPAFYTVLVDFWAQIPINERVKHINTDIYKQLCDEIVKVIEDGIHQGVFKPVDAR
                     LAAHAIVAMVMNVAIQWTFNHELYNIDHLAKTCMKMIMSYLEAKPKLAGQ"
     gene            complement(1061..>1863)
                     /locus_tag="PLV81_15870"
     CDS             complement(1061..>1863)
                     /locus_tag="PLV81_15870"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOM11619.1"
                     /translation="VLTDDIKEYVPTEKVIQELDTLLKSNPALDIITFSGSGEPTLHS
                     GIGTIIDHIKSNYRYTIAVLTNGTLLWKREVRERLYHADIVIPSLDAATEHAFKKICR
                     PHKHLSLQKIIDGIALFRKEFTGLLILEIFIVPGINDLPSELEALAKVARSIHPHRMQ
                     LNYLDRPGTEEWVQRASMETLKQYASVFAPLPVDIPGTPEYRLLELKLPLKEMILSTI
                     ERRPSIVDDLAYSLGKDKLVVQQILDDMVKQGVVEYFDEARGRFYKKI"
BASE COUNT          517 a          388 c          339 g          619 t
ORIGIN      
        1 tcaccattga tgtcattaac aatttgcctg taaccaggaa ggaactggaa cgcatgcggg
       61 aacgaattga acgctcctca aactatgata gcattgctga tttttttata gaaaatcctg
      121 accctgctgc tgagggtatg ggtttggggc tttccatggt agtggttctt ttaaaaagtg
      181 ttgggatacc gtatactaat tttacattat ataccgattt taaaacaaaa acaattgccc
      241 gtattatagt acctttaatg taggataaaa atataggatc ctttaccaag tactttaatt
      301 actgtcccgc cagttttggt ttggcctcta aataagacat tatcattttc atacaggttt
      361 ttgcaaggtg gtctatgttg taaagctcgt gattaaatgt ccactgtatc gcaacattca
      421 taaccattgc cacaatagca tgcgcagcta acctggcgtc aactggctta aaaaccccct
      481 ggtgtatacc atcttcaata acctttacta tttcatcgca taactgtttg taaatatcag
      541 tattaatgtg tttaactcgt tcattaatgg gaatctgtgc ccagaaatca accagcaccg
      601 tgtaaaatgc aggatgacgt tttgctgtca caataaaagc aatgacgata gctcgcatac
      661 ggcgttcggg tgtacggtac tttgcagccc gcttgttaat atttttttta atgataatat
      721 aaatctgttc cagcaaatag atgagtatat cttctttgga tttgaaatag tgcagtacac
      781 caccttttga caatcccgca ttttttgcaa tgtcatccaa tgtaaagtta ttataccctt
      841 ttgcagaaat agcttcatat gctgctgtta ttatctgcgt ttttctttgc tcctcttttt
      901 ccgcccgtgc ttccggtttt aatcgtggca tgtagttttc ctcttactct atattcaatt
      961 ctggtacata acaatgcaca acaaatcaaa attaatgttt atacaagtat ggctattttt
     1021 tataagtttg taaagttttt ttcgtaaatc taaaaaaaaa tcatattttt ttataaaacc
     1081 tacctcgagc ttcatcaaaa tactcaacaa caccctgctt caccatgtca tcaagtatct
     1141 gctgcaccac aagtttgtct ttccccagtg aataggctaa atcatccact atggatggcc
     1201 ttcgttcaat ggtggacagt atcatttctt ttaatggcag ctttaactca agcaaccggt
     1261 actcaggtgt gccgggaata tcaactggta gtggtgcaaa tactgacgca tactgtttca
     1321 gcgtttccat ggaagctctc tgaacccatt cttctgttcc tggtctatcc agatagttaa
     1381 gctgcatccg gtgcggatgt atactgcgtg caacctttgc cagcgcttct aattctgacg
     1441 gcaaatcatt aatacccggc acaataaaaa tttccagaat caaaagccct gtaaattctt
     1501 ttctgaacag ggcaatccca tctataatct tttgcaaaga caggtgctta tgcggtctgc
     1561 atattttttt aaatgcgtgc tctgttgcag catccagtga aggaataaca atatcagcat
     1621 gatacaggcg ctctcttact tcacgtttcc acagcagtgt tccattggta agcacagcta
     1681 ttgtgtagcg atagttactt tttatatgat ctatgatggt gcctatgcca ctgtgtaacg
     1741 ttggctcacc agagcctgaa aaggtgatga tatccagtgc aggattgctc ttaaggagtg
     1801 tatctaactc ctgtatgact ttttcagtgg gcacatactc ttttatgtca tcagtaagta
     1861 ctg
//
LOCUS       DAORYL010000215         1855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_42324_length_1855_cov_2.996667, whole genome shotgun sequence.
ACCESSION   DAORYL010000215 DAORYL010000000
VERSION     DAORYL010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42324_length_1855_cov_2.996667"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1844
                     /locus_tag="PLV81_15875"
     CDS             <1..1844
                     /locus_tag="PLV81_15875"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOM11620.1"
                     /translation="SSLLFVFKTRINEYGQQMQINASAIVNDVKNYLNTGIELVSVAA
                     REGSIDALASLKGKDEIVDVFILKDERNATVLRSLTGKAAGLKSDTLGMIQGMKKGKS
                     FIGDFVYIPEIQRSVVVSAAKNNNLWYCICYDVNAMFQKKFLHYKIGNRGYMLIMDKN
                     GLFLAHPKTDYIGRESLKKYDWGNEAFQEKKVVKYIWEGERKFVYVLENSEPSFAVAQ
                     TVYEKDIGIAIRNTIVFVGLLALGVIILGILASTLILTFAFEPFTVIRDAIYRISEGD
                     ISITPEVIYGNEIGIIATSVKILTSKIGSVIRQIKNYSDELSTASEQMSAGISALSEV
                     ASNQSASTQEITATIEEISANLDSIVINTKDESKSLGEFSSHLQELSQSIFSFLLIME
                     ESAQRTESISNDAQQGQISLQQMNTSMHKIVESSRGISNIAQIIRGIADQVNLLALNA
                     AIEAARAGEAGRGFAVVADEITKLADQIAQSLKDIDVLVKTNEKEIQQGITITENTVA
                     AISNIIKGIESISHEVNTMGQKIKEQSEIFNETKQVWDSTESLSRQILDSVEDQKSAI
                     DEIVRSINTINSLTQNVAGTAEEMNSSAENLASMAQQLKAMLEYFKI"
BASE COUNT          648 a          278 c          405 g          524 t
ORIGIN      
        1 cttcatcatt attatttgtg tttaaaacac gtattaatga atatggccag cagatgcaaa
       61 taaatgcatc agctattgtt aatgatgtga aaaattatct gaatacaggt atagaattag
      121 tttctgttgc agcacgagaa ggaagtattg atgcattggc ttctttaaaa gggaaagatg
      181 aaatcgttga tgtatttata ctgaaagatg aaagaaatgc aacagtactg cgttcattaa
      241 ctggaaaagc tgctggtctg aaatcagaca cattggggat gattcaaggc atgaaaaaag
      301 ggaaaagttt tattggagat tttgtatata ttcccgagat acaaaggtca gttgtggtga
      361 gtgctgctaa aaacaataac ctgtggtatt gcatttgcta tgacgtaaat gccatgtttc
      421 agaaaaagtt tttacattac aaaattggta accgtggata tatgctgatt atggataaga
      481 atggtctatt tttagctcat ccaaagacag attacatagg cagggaaagt ttaaaaaaat
      541 atgactgggg taatgaagct ttccaggaaa agaaggtggt taaatatatc tgggaaggtg
      601 aaagaaaatt tgtttatgta ctggagaata gtgagccatc gtttgcagta gcacaaacgg
      661 tctatgaaaa ggatattggg atagctattc gtaataccat agtatttgtt gggttgctgg
      721 cattgggtgt tatcatatta ggaatacttg caagcacgtt aatattaaca tttgcatttg
      781 agccttttac tgttattcgt gatgcaatat acaggatatc agaaggcgat atatctataa
      841 ctccggaagt gatctatggc aatgaaattg gcatcattgc aacctcagtt aaaatattaa
      901 cttcaaaaat tggttcagta atacgtcaga taaaaaatta ttctgatgag ttatcaacag
      961 cgtcagaaca aatgtctgct ggtataagtg cactgtcaga agttgcatca aaccaatcgg
     1021 catcaacaca ggagatcact gctacgattg aggaaatatc ggcaaacctt gattcgatag
     1081 taatcaatac aaaagatgag tcaaaaagtt tgggtgagtt ttccagccat ttacaggaac
     1141 tatcgcaatc aattttttca tttttactga ttatggaaga atcagcacaa agaacggaat
     1201 ccataagcaa tgatgcccag caggggcaga tatcactgca acagatgaac acaagcatgc
     1261 ataagattgt agaaagctca cgtggcattt caaacattgc ccaaattatt agggggatag
     1321 ctgatcaggt taacctgctt gcactgaatg ccgccattga agcagctcgt gcgggggagg
     1381 caggaagggg ttttgctgtg gtggctgatg aaataaccaa attagctgat cagattgcac
     1441 agagccttaa agatattgat gtgctggtta aaaccaatga aaaagaaata cagcagggta
     1501 ttactattac tgaaaataca gtcgctgcca tatcaaatat aattaaagga attgaatcta
     1561 taagccatga agtaaatacg atgggtcaga agattaagga acagagcgaa atattcaatg
     1621 agactaaaca ggtatgggat agcactgaat cactttcccg acaaatactt gattctgttg
     1681 aggaccagaa aagcgctatt gatgaaatag tgaggtcaat caataccatt aattcattaa
     1741 cccagaatgt tgcaggtaca gcagaagaga tgaattcaag tgctgagaat ttagcttcaa
     1801 tggctcaaca actcaaggca atgcttgaat attttaaaat ttgatttgcc tttca
//
LOCUS       DAORYL010000216         1842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_42722_length_1842_cov_7.588137, whole genome shotgun sequence.
ACCESSION   DAORYL010000216 DAORYL010000000
VERSION     DAORYL010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42722_length_1842_cov_7.588137"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..663
                     /locus_tag="PLV81_15880"
     CDS             274..663
                     /locus_tag="PLV81_15880"
                     /inference="COORDINATES: protein motif:HMM:NF013810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11621.1"
                     /translation="MKLIKLTNILIFLLFLSFSTIYCGGGGGTSSDEVTLPDYRTRPI
                     VFVHGFMGSASQFESQAQRFIANGYNPAWIKVFEHDTTNNDPLTILNQVVELDTVISQ
                     VLTETGADKVNLVSHSRGGYNIYVHIF"
     gene            825..1700
                     /locus_tag="PLV81_15885"
     CDS             825..1700
                     /locus_tag="PLV81_15885"
                     /inference="COORDINATES: protein motif:HMM:NF037036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11622.1"
                     /translation="MSHVQICTAKESFIEMYKFFHNEQPLETEIPVSQENTITIAGRV
                     LYFQENTGATGTSKIYKTSDGHRTSNAIETYNLNANDKGYWGPISVDNNFAYEFEFTH
                     PDGTKHIFYKETLTNNDYFLRLLTGNPNKQGLSDLMPKTDKHVNVIVMRDKEFWGNQD
                     NANDVLNVKFDSDIHNVITPQAAARDHRLIALFLMDWGSDKTSSTTTPDQQTDLSSPI
                     QVFHTYSQGGDPTRAAVFLSGLDLYMPALSDSSGTITFELTSRGGGGKKQIINVPNWR
                     SIDATISVLFNDYVQ"
BASE COUNT          639 a          302 c          297 g          604 t
ORIGIN      
        1 tataataaat gtttatatat tgatgataac atttaaaatt tatttcataa attaataaaa
       61 atacatattt tcccaataaa attgctaata atacagtttt tacgaaaagt tccaattgac
      121 tgtattcatc taaatgaatg ctacataaaa caatagacaa tattctgatg acgcatccat
      181 ttcttatggg acatttgtta ttattcttat tgacaaattt atatattaat gtacatacat
      241 taatatataa atattttttc aggaggtaaa gctatgaaat taataaaatt aacaaacatt
      301 ttgatttttt tactatttct atctttttct acgatatatt gtggcggtgg tggcggaact
      361 agttctgatg aagttaccct gcctgattac agaacaagac ctattgtttt tgttcatggt
      421 tttatgggtt cagcttcaca atttgaatca caggctcaaa ggttcatagc caacggatac
      481 aaccctgcct ggataaaagt ttttgaacat gatacaacta ataatgatcc acttaccatt
      541 ttaaatcagg ttgttgaatt agatacggtt attagccagg ttttaacaga aactggggca
      601 gacaaagtta atcttgtaag ccattcccgt ggaggatata atatatatgt acatattttt
      661 taaaaaacag ttcatatgct tcaaaaattg cacgctacgt acatattgac agtactaaag
      721 accttgtacc ggcaaaagga gtcccggcac tagtactatg gagtgaacct ggacttctcc
      781 ctcaaagtga tatatctgaa gcaactaaca tacattttga taatatgagt catgtgcaaa
      841 tatgcactgc aaaagaatca tttattgaaa tgtataaatt ttttcacaat gaacaacctt
      901 tagaaaccga gatcccggta tcacaggaaa acactataac tattgctgga cgagttcttt
      961 attttcagga aaatacggga gcaactggca catcgaaaat atacaaaaca tcagatggac
     1021 atagaacgag taatgcaata gaaacttata atttgaatgc caatgataaa gggtattggg
     1081 gaccaatatc agttgataat aactttgctt atgaatttga atttacacac ccagatggta
     1141 ccaaacatat attttataaa gaaacattaa ccaataatga ttattttttg cgtcttttaa
     1201 caggtaatcc caataaacag ggtttaagcg accttatgcc taaaacagat aaacatgtta
     1261 atgttatagt aatgagggat aaggaattct ggggaaatca ggacaatgcc aatgatgtac
     1321 tgaatgtgaa atttgatagt gatattcata atgtcattac tccacaagct gctgcacgcg
     1381 atcaccgttt aattgcacta tttttaatgg attggggaag tgataaaaca tcttcaacaa
     1441 caacaccaga ccaacaaacc gacctttcaa gccccattca ggtttttcat acctattcac
     1501 agggtgggga tccaacaaga gcagcagtat ttttatcagg tcttgatctt tacatgcctg
     1561 ccttatcaga cagtagcggc actatcacgt ttgaacttac ctctcgtggt ggaggtggta
     1621 aaaagcaaat aataaatgtt cctaactggc gttcaattga tgctaccata tcagtacttt
     1681 ttaatgatta tgttcaataa gactataccc tttcgccatt tcaatcggcg aaagtgtata
     1741 gccagcggta taaattcatg ataattaata atttttttaa attttaatta attttttact
     1801 tgccttttta gcattttagt atatatttaa attactaagt tt
//
LOCUS       DAORYL010000217         1841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_42753_length_1841_cov_5.670773, whole genome shotgun sequence.
ACCESSION   DAORYL010000217 DAORYL010000000
VERSION     DAORYL010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42753_length_1841_cov_5.670773"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1811)
                     /locus_tag="PLV81_15890"
     CDS             complement(<1..1811)
                     /locus_tag="PLV81_15890"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOM11623.1"
                     /translation="MDCSTLDFTKENPVELKGEWKFKMYEDNPRYALPDLDDSQWDTI
                     NIPNYWNVKKYKGEGFGWFRLKVKINDSFWQSNPAIYLQGANTAYELYCNGEKVLYAG
                     IAGVKRETSVPSLKPQLQPLHIHHNPEEIVIAVKVSNFYHRGGGLNKSIVIGSFDSIS
                     KVLWQRDFSATLILGILIITAIYHIILFFGRMKDKTSLWFSIFCFAVFSHSFVVGNYL
                     EQIFSGQRIYFIRFAIEYASLGLGWSGLALYMYSLFPRETGKIFVFSLVAIGVVFICV
                     PIILPPQIFTKLISVFNLFLLLSLIGSFYVLIKASFRKREDALLILSGFIIFMISILN
                     DMLHNLFIIRTAFISEYGLAFFILFQSSVLSLRFSRAFKLSEYLSNNLNEEVNKKTAE
                     LLQKTEEALNARQETEETYKKISVLYKQMSHEIHLAKIVHDRIIPKECSTFPGIKVTI
                     YTNPFMEIGGDFYDITKLEEGQTRLFVADATGHGISASLATILIKVEYDKIKDSCAKP
                     SEIFGRLNDIFFEYYRSLKMYITGVVIDINTKHNIIMYASAGHPDQIIVKNGNLHELS
                     RTGPAIGISRTPGYEDRTINFNHKDTLFLFTDGIFEE"
BASE COUNT          630 a          353 c          285 g          573 t
ORIGIN      
        1 aattcttcaa aaataccatc agtaaataaa aacaatgtat ccttatggtt aaaattaatg
       61 gttcgatcct catagccagg tgtacgtgaa ataccaatgg caggcccggt acgggataat
      121 tcatgcaaat taccattttt aactataatc tggtcaggat gacccgctga agcatacata
      181 atgatattat gttttgtatt aatatctatc acaacaccgg ttatatacat cttcaaagat
      241 ctataatact caaaaaatat atcattaagc ctaccaaata tttcagaagg ttttgcacag
      301 ctgtccttta ttttatcata ttcaactttt attaaaattg ttgccaatga ggcactgata
      361 ccatgtcctg ttgcatcagc aacaaacagt cgagtttgtc cctcctccag ctttgtaata
      421 tcataaaaat caccaccaat ctccataaag gggttagtgt atattgttac tttaatgcca
      481 ggaaatgtac tgcattcttt tgggataatc ctgtcatgca caatttttgc tagatgtatt
      541 tcatgtgaca tctgcttata caatacagat atttttttat atgtttcctc cgtttcctgg
      601 cgtgcgttca aagcttcttc cgttttttgt aacagttctg ctgttttttt gttaacctct
      661 tcattaagat tgtttgaaag atattctgaa agtttaaatg cccgtgaaaa acgaagtgat
      721 aacacagaag attggaacag aataaaaaag gcaaggccgt attctgaaat aaatgcagta
      781 cgtataataa aaagattgtg caacatatca tttaagatgc ttatcatgaa tataataaaa
      841 ccagataaaa ttaataatgc atcttcgcgc tttctaaaag aagctttaat tagtacataa
      901 aaactcccaa tcaatgaaag caatagaaat agattgaaga cactgataag ctttgtaaaa
      961 atttgtgggg gtaatataat tggtacacat ataaacacaa cccctattgc taccagagaa
     1021 aaaacaaata ttttgcctgt ttctcgggga aaaagtgaat acatatacaa cgccaggcct
     1081 gaccatccca atcccaaact tgcatattca atcgcaaatc gtataaaata gatcctttga
     1141 ccgctaaaaa tttgttcaag atagttcccc acaacaaaag aatggctgaa aacagcaaaa
     1201 cagaaaattg aaaaccacag agaagtttta tccttcatcc gcccaaaaaa gagaatgata
     1261 tggtaaatag ccgttattat taaaatacca agaataagcg ttgcagaaaa atctctttgc
     1321 cagaggactt tactaatgct gtcaaaagag cctataacaa tagatttatt aagcccacca
     1381 ccccgatgat aaaaattaga tacttttaca gcaatgacaa tttcctctgg attatggtgt
     1441 atatgcaaag gttgtaattg tggttttaag ctgggaacag atgtttccct ttttacaccg
     1501 gcaatcccgg catacaatac tttctcacca ttacaataga gttcatacgc tgtatttgcc
     1561 ccctgaagat aaatagcagg attgctttgc caaaaactat cgttaatctt aacctttaat
     1621 ctgaaccatc caaatccttc tcccttatac ttttttacgt tccagtaatt aggaatgttt
     1681 atcgtatccc attgtgaatc atcaagatca gggagggcat atctaggatt atcctcatac
     1741 attttaaatt tccattcgcc ttttagctct accgggtttt cttttgtaaa atctaatgtt
     1801 gaacaatcca taaccccacc agttgcagca tgaggtgaga a
//
LOCUS       DAORYL010000218         1834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_42960_length_1834_cov_10.635750, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000218 DAORYL010000000
VERSION     DAORYL010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42960_length_1834_cov_10.635750"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="PLV81_15895"
     CDS             complement(<1..1108)
                     /locus_tag="PLV81_15895"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOM11624.1"
                     /translation="MHCQKRYKHLFQTSSVLSLMAVIIIFSVSVVNAQPLTLKQAITT
                     AIQNNNTYKAALLKVEENRYKVRESWGMLWPQLSTDVAYTRQWYEAGFQAQIAGQYDV
                     KIINGQIAVNPGAFYNSLQASRNGYIASEYEARSIKANTIITTLRLYYQVLLTKELIS
                     MHEESLKALEENLRVVTVGYQKGTFSKLDFLRAKVAYNNEKTQLINAKNDYLTVCATF
                     NIQLNRDINDPVNLDESVLVVSNDDLQYLSLEEEKETALIKELVGLSLKNRPELLQLT
                     YSKKALAYASDAASSVYLWPSLFVSGSYGSSKSISKESSGFQPTGDPMIDPILAAMSQ
                     GMSESFAPKGWNDHWMITFGATYTWGAWSPIDSSH"
     gene            complement(1086..1829)
                     /locus_tag="PLV81_15900"
     CDS             complement(1086..1829)
                     /locus_tag="PLV81_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOM11625.1"
                     /translation="MQSFKYSKETRDVILNAAKKEFAERGYSGARMSSIAKRAGVNQA
                     LIHYYFSSKEKLYREVLEFLFHHEESKIVEEYFKQDVLTPPEALYVAIYILVYLHHDA
                     IDPETNRIISWEIAEGGETFKKLLIEYLYPQLDRITLIIQQGIEQGYFETSDPFLIVF
                     NLVNFVIAFENNKQFLKDTPWYARFYGGDDGRRLFEYTLTATFKALTPKGKLFTLPHI
                     SNELQQKLHSIIEKLKSTQGERYALSKEV"
BASE COUNT          504 a          403 c          328 g          599 t
ORIGIN      
        1 catgtgatga atcaattggt gaccatgccc cccacgtata ggtagcacca aaggttatca
       61 tccagtgatc attccagcct ttaggtgcaa agctttctga catgccctga ctcattgccg
      121 ctaaaatggg atctatcatt ggatcgcccg ttggttggaa gccactgctt tctttagata
      181 tggatttgct tgaaccataa ctgccgctta cgaataaact tggccacagg tatacggacg
      241 atgcagcatc tgatgcatat gctaatgctt ttttgctata tgtcagctgc agtaactcgg
      301 gcctgttttt taatgacagg ccaaccagct ccttgataag agctgtctcc ttttcttctt
      361 ccagtgaaag atactgtaag tcatcattgc ttaccaccag cactgattca tccagattaa
      421 ctggatcgtt aatatccctg tttaactgta tgttgaaagt tgcacacacc gttaaataat
      481 catttttagc attaataagt tgtgtctttt cattattgta ggcaaccttt gcacgcaaaa
      541 aatctaattt agaaaaggtg cctttttgat atcctacagt gacaacacgt aagttttctt
      601 ctaatgcctt cagcgattcc tcatgcatgc tgatcagttc tttggttaat agtacctgat
      661 agtatagcct caatgtggtt atgatagtat ttgccttgat tgaccttgcc tcatattcac
      721 tggcaatata gccattacgg gaagcctgca ggctgttata aaacgcacca ggatttactg
      781 ctatctgccc atttattatc tttacatcat actgtcctgc aatctgcgcc tgaaagcctg
      841 cttcatacca ctgccgtgta taagcaacat cagttgaaag ctgtggccac agcatccccc
      901 agctttcacg caccttatag cggttttcct caacttttaa aagagcagct ttataagtat
      961 tattattctg tatggcagta gtaattgcct gctttagtgt aagcggttgt gcatttacca
     1021 ccgatacact gaaaataata atcactgcca tgagtgaaag aacgctactt gtttggaata
     1081 aatgtttata cctcttttga caatgcatac cgttctccct gtgtactttt aagcttttca
     1141 ataattgaat gtaatttttg ctgtaattcg ttgcttatat gtggcaacgt aaagagcttc
     1201 cccttggggg tcagagcctt aaaagtggcc gtgagtgtat attcaaacaa ccgcctgccg
     1261 tcatcaccgc cataaaatcg ggcataccac ggagtatctt tcaaaaactg cttattattt
     1321 tcaaaagcta ttacaaaatt gaccagatta aaaactatca aaaaaggatc actggtttca
     1381 aaatacccct gttcaatacc ctgttgaatt atcaatgtaa ttctatctaa ttgtggataa
     1441 agatattcta ttaaaagctt tttaaatgtt tccccgcctt ctgcaatttc ccatgatatt
     1501 atcctgtttg tttcaggatc tattgcatcg tgatgcaggt acacaagaat atagattgcc
     1561 acatacaacg cctcaggggg agttagcacg tcttgtttaa aatactcttc aactattttt
     1621 gactcctcat gatgaaacaa aaattcaaga acctccctgt atagcttttc cttactagaa
     1681 aagtaatagt gtatgagcgc ctggttgaca cctgcacgct ttgctattga gctcatccgc
     1741 gcaccactgt agccccgttc agcaaattct ttttttgcag cattaagaat tacatcacgg
     1801 gtctcttttg aatatttgaa tgattgcatt ctgt
//
LOCUS       DAORYL010000219         1813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_43662_length_1813_cov_9.590444, whole genome shotgun sequence.
ACCESSION   DAORYL010000219 DAORYL010000000
VERSION     DAORYL010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43662_length_1813_cov_9.590444"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /gene="panB"
                     /locus_tag="PLV81_15905"
     CDS             complement(<1..613)
                     /gene="panB"
                     /locus_tag="PLV81_15905"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003249639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOM11626.1"
                     /translation="MSSIKNIKDFAKMKQSGEPIAMITCYDYAFARVVSETDIDVILV
                     GDTLGMVVAGFDTTIPVTLDQMIYHSSMVRRGAPQTFIVTDLPFMSYHVSIEDTMRNC
                     GRIMKECGANAVKLEGGRDFVPVVEALTKASIPVMGHLGLTPQSVHKLGGYSVQGKDE
                     EQRKIIMEDALLLQKAGAFALVLEMVPEQLAKEITEALDIPTIG"
     gene            complement(601..1062)
                     /gene="folK"
                     /locus_tag="PLV81_15910"
     CDS             complement(601..1062)
                     /gene="folK"
                     /locus_tag="PLV81_15910"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOM11627.1"
                     /translation="MEAYAYILLGSNMGDSYRYIDAAIEKLSTLEKTKIIQQSSVIVT
                     KPLEYTKQPDFVNTVVALLTQLPPHTLLEKLQKIEYSLGRERTIPKGPRTIDCDILLY
                     DDMVCNTPELTLPHPQIYTRPFAAQLLLEINNDIIDPLSHKPLREVVLCHQ"
     gene            complement(1062..>1813)
                     /locus_tag="PLV81_15915"
     CDS             complement(1062..>1813)
                     /locus_tag="PLV81_15915"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM11628.1"
                     /translation="IVRNPHFEEIGQEPLVPCPRCVLTKCQCGGQPPYYIEKDGTITD
                     CYCRDIRMKINRIKAIYLRSGIDKKYQWRFFNSFEANSKLASNAKNVAYDIVSKFPDV
                     RKGLFLWGNPGTGKTLLSSIILTELIIRHAIEGRFIKISRTFFNKLRATFAEGSATYG
                     TAQEIEYELENADVLVVDDFGVQRDSPWEQETLYNLVDARYEAEKFTIFTSNVDPHRA
                     LSEIAGGRVLSRIKEMCTILELTGPDYREKL"
BASE COUNT          474 a          395 c          392 g          552 t
ORIGIN      
        1 tgccaatggt gggtatatca agcgcttcag ttatttcttt tgcaagctgc tcgggtacca
       61 tttcaagcac taacgcaaat gctcccgctt tttgtaacag caacgcatct tccatgatga
      121 ttttacgctg ctcttcatcc ttcccctgaa cagaatatcc acctaattta tgaactgact
      181 gcggtgtaag ccctaaatgc cccataaccg gtattgaagc tttggtcagt gcttcaacaa
      241 caggaacaaa atcccttccg ccttctaatt taacggcatt ggcaccgcat tctttcataa
      301 tcctgccaca gttgcgcatg gtatcttcaa ttgatacgtg gtagctcatg aacgggaggt
      361 cagttactat aaaggtttgt ggtgcaccgc ggcgtaccat gctgctgtga taaatcatct
      421 ggtcaagggt tactggtatg gtggtatcaa agcccgcaac aaccatcccc aatgtatcgc
      481 caaccagtat gacatcaata tcagtttcgc tgacaacacg tgcaaaggca taatcataac
      541 aggttatcat ggcaatgggc tcgcctgact gtttcatctt tgcaaaatct ttgatgtttt
      601 ttattgatga catagtacta cctcccgtaa tggtttgtga gaaagcggat caatgatatc
      661 gttgtttatt tccagcaata gctgtgctgc aaatggtcgt gtgtagattt gtggatgagg
      721 cagggtaagt tccggtgtgt tacacaccat atcatcgtaa agcaaaatat cgcaatcaat
      781 agtgcggggg cctttaggga tggttctttc ccttcccagt gaatactcta ttttttgaag
      841 cttttcaagt agcgtgtgtg gtggcagttg cgtgagcagc gcaacaactg tattgacaaa
      901 atcgggctgc ttggtatatt ctaaaggttt agttactatt acactgcttt gttgtataat
      961 ttttgttttt tccagtgtgg acaacttttc aatagcagcg tcaatatacc ggtaactatc
     1021 gcccatattg cttcccagta aaatgtatgc atacgcttcc attataattt ttccctgtaa
     1081 tcgggacctg ttagctctaa aattgtgcac atttccttaa tgcgcgacaa tactctgccg
     1141 ccagcaattt cagacagtgc acgatggggg tcaacgttgg atgtaaatat ggtaaatttt
     1201 tcggcctcgt agcgtgcatc aactaagttg tacagggttt cctgctccca gggggagtca
     1261 cgctgtacac caaaatcatc caccacaagt acatcagcgt tttccagttc atattcaatt
     1321 tcctgcgcgg taccgtatgt ggcagagcct tcggcaaatg ttgcacgaag tttattaaaa
     1381 aatgtacggg aaatttttat aaaacggcct tcaatggcat ggcgtatgat gagttcggtt
     1441 aatataatag aagaaagcag cgttttgccg gttccgggat ttccccataa aaaaaggccc
     1501 ttgcgtacat ccggaaattt tgaaactata tcgtaagcca cattttttgc gttggaggct
     1561 aattttgaat tggcttcaaa ggaattaaaa aaacgccact gatatttttt gtcaatacct
     1621 gaacgcagat atattgcttt tatgcggtta atcttcattc tgatgtcacg gcaatagcaa
     1681 tcggttattg tgccatcttt ttctatatag tacggaggct gtccgccgca ctggcattta
     1741 gtcaacacac agcgggggca gggtaccagc ggttcctggc caatctcttc aaaatgggga
     1801 ttgcgcacaa tac
//
LOCUS       DAORYL010000220         1784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_44680_length_1784_cov_15.030654, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000220 DAORYL010000000
VERSION     DAORYL010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44680_length_1784_cov_15.030654"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..822
                     /locus_tag="PLV81_15920"
     CDS             106..822
                     /locus_tag="PLV81_15920"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOM11629.1"
                     /translation="MKDVAVKESAIFKQIEHWRRQKQQVVEDKEKANTLPFITISREY
                     GCGGFDIAVRLTDILNKEYHATPIWAAYDKQILDKITSDLGLTKELTETLTNTARSKM
                     TDLFQTTFSKFPPQVAVYRKLAEVVRTLAINGHVVIVGRAGNVITRGMPYGLHVRIVA
                     PMSYKVKRIAAKLKVTQAEAREIIEKKGVERESFIKNFLKFDPEDPHNYDIVINNENY
                     AIDELARLIIDAMKRKGIFA"
     gene            833..1306
                     /locus_tag="PLV81_15925"
     CDS             833..1306
                     /locus_tag="PLV81_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11630.1"
                     /translation="MLTFTCNDTAIVYNPEKNAILCVSNTDGKKLWVKKLSEPMAIQN
                     VLFDDRFYYIACRTGDTEGMFLTVARGNGTTVWFIPGRTFLEVLYNEFLYLIFVDEDD
                     RYFLIKVEREEGTKLWYHQIDSDLYYYHFKEDGILLHYASGKKEKITYSGKRIVY"
     gene            1584..>1784
                     /locus_tag="PLV81_15930"
     CDS             1584..>1784
                     /locus_tag="PLV81_15930"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM11631.1"
                     /translation="MKAIGVPTEAASIYNQALALSNQGNYVQAIETYMHALEVFPQFI
                     EAYNNIGEIYSRMGNSEKAIQVY"
BASE COUNT          608 a          269 c          391 g          516 t
ORIGIN      
        1 ttttattgcg tattttataa atgcagtaag gaggcaattg cacatgcaaa agcctttatg
       61 gttatttttt gtgggcgata gtccttataa agaaaggagg cccctatgaa agatgtagcc
      121 gtaaaagaaa gcgccatttt taaacagatt gaacactggc ggcggcagaa acagcaggtg
      181 gtcgaagata aggagaaggc taatacgctg ccctttatta ctatttctcg tgaatatggt
      241 tgtggcggat ttgatattgc agttaggctg actgatatat tgaataaaga atatcatgct
      301 acaccaatat gggcagcata tgataaacaa attcttgata aaataacttc ggatttggga
      361 ttgacaaaag aattaaccga aacactgaca aatactgcac ggagcaagat gaccgatctt
      421 ttccagacta cgtttagtaa gttcccacca caagtagcag tatatagaaa attagccgag
      481 gtggtacgta cactagcaat aaacggacac gtggtaatag tgggaagggc aggcaatgtt
      541 atcacccgtg gtatgccgta tgggttacat gtacgtatag ttgcgccaat gagttacaaa
      601 gttaagcgca tagcagcaaa gcttaaagta acgcaagctg aggcacggga gataattgaa
      661 aaaaagggag ttgaaaggga aagctttata aaaaattttc ttaaatttga tcccgaagac
      721 ccgcataact atgatattgt tatcaataac gaaaattatg ctatagatga gttggcgcgt
      781 ttaattattg atgccatgaa gcgaaaaggg atttttgcat aacactttgg atatgctgac
      841 ttttacttgt aacgacactg caattgtata taatccagaa aagaatgcaa tactgtgtgt
      901 tagcaacacc gatggcaaaa agttatgggt aaagaagctt tcagaaccca tggctattca
      961 gaatgtattg tttgatgatc gtttttacta tattgcctgt agaactgggg ataccgaagg
     1021 catgttttta acagtggctc gaggtaatgg aaccactgtc tggttcatcc cagggcgtac
     1081 gttccttgag gttttatata atgagtttct gtatcttata tttgtggatg aggacgatag
     1141 atattttctc attaaagtgg aacgtgaaga aggcaccaaa ttgtggtatc accagataga
     1201 tagtgatctg tattactatc atttcaaaga agatgggatt ttgcttcatt atgcttcagg
     1261 aaagaaagaa aagataactt atagtggcaa gcgcattgtg tattaatggt tttaaaatga
     1321 taatatcatt gatataatat taaaggatta agcgaagata aaaaaaagat catagctttt
     1381 acaggtcatt ttgaacgtat ttcataatct taaaatatta agtatagatt atgggtcaag
     1441 cccggaatga catttataaa ataatttttc aataacgcta tcgccaaaaa actactgtga
     1501 tgaaattcta aatgaaaatt taaattttac cgataggtat atcgtaggtg ataaatgcat
     1561 attactgagc aagaggtttg ggtatgaaag caataggtgt accaactgag gctgccagca
     1621 tatataatca agcgcttgca ttgtccaatc agggaaatta tgtacaggca attgaaacat
     1681 atatgcatgc gcttgaagtg ttcccacaat tcattgaggc atataacaac attggcgaga
     1741 tatattcccg catgggcaat agcgaaaaag ccattcaggt gtat
//
LOCUS       DAORYL010000221         1739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_46339_length_1739_cov_6.084917, whole genome shotgun sequence.
ACCESSION   DAORYL010000221 DAORYL010000000
VERSION     DAORYL010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46339_length_1739_cov_6.084917"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PLV81_15935"
     CDS             complement(<1..421)
                     /locus_tag="PLV81_15935"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HOM11632.1"
                     /translation="MKACVTGATGFIGGNLVKALLAKKHSVKALILPNDPKIPELKKN
                     KVEVVQGDIRDLESVKKAVKGCDVIFNCAAVVTDWAPQRLFEEVTVGGTKNVCEASVW
                     AKVKRLVDISTNDVFGLREDVVMDESFPLEPWGEPYPD"
     gene            complement(580..741)
                     /locus_tag="PLV81_15940"
     CDS             complement(580..741)
                     /locus_tag="PLV81_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11633.1"
                     /translation="MGKIGGQSIVKVIVQKRKKGSEHKGPGNLLLMAYMADSFAGFQV
                     KDLIGEVIE"
     gene            1142..>1739
                     /locus_tag="PLV81_15945"
     CDS             1142..>1739
                     /locus_tag="PLV81_15945"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOM11634.1"
                     /translation="MARKLRVQLPGLTYHITSRCIEWKNYLQEDYFKACFVQVLNKAK
                     AKYHFKLIAFCIMDNHIHLVIHTLDDGAPISRIVQYIKARFAELYNKITGRTGPFWNE
                     RYKDSIVQFAKDGFHYLLWLLWYLAYNPIRRRLCSDPTKYHYSSIQAYLTEEANIGVP
                     IDHHDYFVKLGETFAKRVARFMQYEEYYRKKYSMILEWV"
BASE COUNT          507 a          392 c          252 g          588 t
ORIGIN      
        1 aatctggata cggttcgccc caaggttcta acggaaacga ttcatccatc acaacatcct
       61 cacgcaaccc aaacacatca ttggtggaga tatcaacaag cctttttacc ttagcccaca
      121 ctgaagcctc acacacattt tttgtaccac caacggtaac ttcttcaaat agtctttgcg
      181 gtgcccagtc agtcactaca gcagcacagt taaaaataac atcgcacccc tttactgctt
      241 tttttacact ttcaagatcc ctaatatcgc cctgcacaac ctctacttta ttcttcttta
      301 gttccggtat cttgggatca ttgggtaata tcagtgcctt tactgaatgt ttttttgcca
      361 gcaatgcttt aactaaattt cctccaataa atccagttgc accagtaaca catgctttca
      421 ttgaaaacct ccctgacttt acgtttgtac attttagtca tttattttct tttcatgaaa
      481 ctaatttcta tttctattta attaaatact catgctactt atattgaagt atccctatat
      541 tatacacaaa atttcaagta aaatatatac ttaataattt cattcaataa cttcccctat
      601 taaatctttc acttgaaaac ctgcaaaaga gtctgccata taagccatta aaagtaaatt
      661 gcctggtcct ttatgctctg accccttttt acgcttttgc acgattactt ttactatgct
      721 ctgaccccct attttcccca tatttttacc aatcctctct acaattcacc tgctccattc
      781 agtattcttc tccatataaa gttttaagga tctgacccca ttctatttac tttacccttt
      841 actatcttta ctatgctctg accccatgtt ggatcccttg ttctgtcgat gttgcgtatt
      901 tatgtcatac ttagcattat ttgtaatcat tgcttgatat atcttaaaat taataggctc
      961 tgaccctttg tttctgtaat tcatctctta tctcttatta tgctctgacc ctattcgatt
     1021 cataaaaaaa tcttcaaaat cttaaaaaat ttttcactat acccgttcaa ttttcaaatc
     1081 acaagcgtta tatacagtaa aggctttatt ttcttaccta ctacatacaa ggaggaatac
     1141 catggcacgg aaattacgtg tacaactccc aggtttaacc tatcacatca cttcccgctg
     1201 cattgaatgg aaaaactacc tgcaggaaga ctatttcaag gcatgctttg tacaggtttt
     1261 aaacaaagct aaagctaagt atcactttaa gctcattgcc ttctgtatta tggataacca
     1321 tattcatctt gtcattcata cacttgatga tggtgctcct atatcccgta ttgtgcagta
     1381 tataaaagcc aggtttgctg aactgtataa taaaatcacc ggcagaaccg ggccattctg
     1441 gaatgaacgg tacaaggaca gtatcgtaca gtttgctaaa gatggctttc attatttgtt
     1501 gtggttattg tggtatctgg catataatcc catacggagg aggttatgct ctgaccctac
     1561 aaaataccac tacagcagca tacaggcata tttaactgaa gaagcaaata taggtgtacc
     1621 catagaccat catgactact ttgtgaagct cggggaaacg tttgccaaaa gggtggcaag
     1681 atttatgcag tatgaggaat attacaggaa gaagtattcc atgatacttg agtgggtgt
//
LOCUS       DAORYL010000222         1671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_49098_length_1671_cov_3.358911, whole genome shotgun sequence.
ACCESSION   DAORYL010000222 DAORYL010000000
VERSION     DAORYL010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49098_length_1671_cov_3.358911"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1671)
                     /locus_tag="PLV81_15950"
     CDS             complement(<1..>1671)
                     /locus_tag="PLV81_15950"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOM11635.1"
                     /translation="IFQKLGREKGSMLLNVTPPDTPTIMGDSSKEWLSDLQFTLLVKE
                     MGWLLGCRSASNNWAVGPQLTANKKAILCSDMQVHQSKMPNDFYLIHVKAGDFEVAGA
                     QVVGLPFVASGFNKHCAWGLTNQGADMVDLFKETIDWKNERYKYNGKWYALGKKKEVF
                     AVKGSKPVEKIIYYVGRKPVLTEVFKDLGFDVSLDWTGFDAIDFRGFFMMNAAKNYDE
                     FMKGAQYIRISPQNVAYADDQGNIAFRVIGSLPLRKKGTGNIIQDGEKVMCNWEGNIP
                     DDKYPMLKNPQRGYIATANNLNVKDYPYDVNATYAPGYRYENIARMLRDKKNLDAEYM
                     KTMQTDTHTVLAGKIKNIVKKYVSREKLEEYMKQAYDMLMQWDGNSSKDSAAASVYNT
                     FYVRFAYQTFVDELGDEVATEYVSERYISMERFFEMVTSGSVFFDDIRTPQKETIGDI
                     AMRAFRETCSMLEDIFGTENVEKWKWGKIHKIQFEHVLGKSALFRPLVNYGPFPFDGD
                     GETNCRAKFLEVAPPFIADLASAPRIIVTFNPQPSGYMMLITGENEHF"
BASE COUNT          462 a          417 c          276 g          516 t
ORIGIN      
        1 taaagtgttc attttcaccg gtgatgagca tcatgtagcc agaaggctgt ggattaaatg
       61 ttacaataat acggggtgca gacgccaaat ctgcaataaa cggcggtgcc acctccagaa
      121 atttagcacg gcagtttgtt tcaccatcgc catcaaacgg gaatgggcca taattgacaa
      181 gtggcctgaa caaagctgat ttccccagca catgttcaaa ctgtatttta tgaattttgc
      241 cccacttcca tttttccaca ttctctgttc caaagatatc ttcaagcata ctgcatgttt
      301 ccctgaatgc acgcattgca atatcaccaa ttgtttcctt ttgtggagtt ctgatatcat
      361 caaagaaaac actgccactt gttaccattt caaaaaagcg ctccatactg atatagcgct
      421 cactgacata ctcagtggcc acttcatccc caagttcatc aacaaatgtc tgataggcga
      481 agcgaacata gaatgtattg tagacagagg ctgcagcgga atccttgctg ctgttaccat
      541 cccattgcat gagcatatcg tacgcctgct tcatgtattc ttccagtttt tcacggctga
      601 catacttctt tactatattt tttatctttc ctgctaatac ggtgtgggta tctgtctgca
      661 tggttttcat atactcagca tcaagattct ttttatcacg taacatgcgt gcaatatttt
      721 cataccggta tccaggcgca taggtggcgt ttacatcata cggatagtct ttaacattga
      781 gattgtttgc agttgcaata tacccacgtt gaggattttt tagcatggga tatttatcat
      841 caggaatatt accctcccag ttacacatta ccttttcacc atcctgtata atattgcctg
      901 tccctttttt acgcaacgga agtgaaccta taactctgaa agcaatattg ccctgatcat
      961 cagcatacgc tacattctgt ggggaaatcc gtatatactg tgcacccttc ataaattcat
     1021 cataattttt tgccgcattc atcatgaaaa agcctctaaa atctattgca tcaaatccag
     1081 tccagtctaa gctcacatca aaccctaaat ccttaaatac ttcagttaac acaggctttc
     1141 tgccaacata gtatataatt ttttcaactg gtttgctacc ttttaccgca aacacttcct
     1201 ttttctttcc cagagcatac cacttcccat tatatttata ccgttcattt ttccagtcga
     1261 tagtttcttt aaacagatcc accatatccg caccctgatt ggtaagcccc catgcacagt
     1321 gtttattgaa ccccgaagca acaaacggca atcccacaac ctgcgctcca gccacttcaa
     1381 aatcgccagc cttaacatga atcagataaa aatcgttggg catttttgac tgatgcacct
     1441 gcatatcact gcacaatatt gctttcttat ttgcagtaag ctgcgggcca actgcccagt
     1501 tgttggatgc tgaccggcaa cctaaaagcc agcccatctc ttttacaagc aatgtaaatt
     1561 gcaaatcaga aagccactcc tttgagctat cgcccataat cgttggagta tccggtggtg
     1621 taacattaag aagcatgctt cccttttccc tacccaattt ctgaaatatc t
//
LOCUS       DAORYL010000223         1652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_49887_length_1652_cov_20.581090, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000223 DAORYL010000000
VERSION     DAORYL010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49887_length_1652_cov_20.581090"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PLV81_15955"
     CDS             <1..219
                     /locus_tag="PLV81_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017826896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HOM11636.1"
                     /translation="WLDTGTVDTLVDAAEFVKVLEKRQGIKIAAIEEIAYHNGWITKD
                     ELERAAELYGKSPYGEHLRKVAAGKIRY"
     gene            228..1631
                     /gene="rfbD"
                     /locus_tag="PLV81_15960"
     CDS             228..1631
                     /gene="rfbD"
                     /locus_tag="PLV81_15960"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015223342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOM11637.1"
                     /translation="MKVIDTALQGVKIIETDYFDDDRGWFTESYNKERFVSFGLDADF
                     IQDNHSYSKKKGTLRGIHFQNNPKAQAKLVRCTKGSILDVVVDLRKGSDTYKKWISCE
                     LSDKNKKQLYIPKGFGHGFVTLEDDVEVQYKVDEYYSKEHDRCIRFNDPALNIDWGVD
                     NPILSEKDATAPFLCDSDVNFSVTVLVTGVNGQLGHDVVQWLNELGITAFGTDLPECD
                     ITNVNSVEEYLLKIKPDVVVHCAAYTAVDRAEQERDRCFAVNVEGTRNIALCCKKLDA
                     KMLYVSTDYVFDGRGDSPYHEDAELAPINFYGYTKAEGEKVVRDLLDKYFIVRTSWVF
                     GINGNNFVKTMLKLAESNKEIKVVNDQIGSPTYSRDLAKLICEMIQTNKYGIYHVSNE
                     GYCSWYEFAHEIFRLAGKKIDVIPISSAEYPVKAKRPHNSRLAKDNLDKNKFSRLPHW
                     KDALARFLSELKGEVDG"
BASE COUNT          528 a          251 c          394 g          479 t
ORIGIN      
        1 tggctcgata ctggcacagt ggatactctg gttgatgctg ctgagtttgt aaaagtgctt
       61 gaaaaacgac aggggatcaa gattgctgca atagaagaaa ttgcctatca taatggctgg
      121 ataacaaaag atgaactgga acgagcagca gagctttatg gaaaatcccc ctatggggaa
      181 catttgcgaa aggttgctgc tggcaaaata cgctattgag gattgtaatg aaggttattg
      241 atacagcatt gcagggagta aaaattattg aaaccgatta ttttgatgat gatcgcggct
      301 ggtttaccga aagctacaac aaagaacgct ttgtaagctt tggcctggat gctgatttta
      361 ttcaggataa ccattcttat tccaaaaaaa agggaacgct gcgtggcatt cattttcaaa
      421 ataatcccaa agctcaggca aaattagtgc gatgcactaa gggcagcatc cttgatgtag
      481 tggttgatct aagaaagggt tctgatactt ataaaaagtg gatttcctgt gaactatcgg
      541 acaagaataa aaaacaacta tatattccaa aaggctttgg tcatggcttt gttacccttg
      601 aagatgatgt agaagtccag tataaggttg atgagtatta ttcaaaagag catgatagat
      661 gcatccgctt taatgatcct gcattaaaca ttgattgggg tgttgataat ccaattcttt
      721 ctgaaaaaga tgcaaccgct ccttttttgt gcgatagtga tgtgaatttt tcagttacag
      781 tgctggtgac cggagtcaat ggccagctgg ggcatgatgt agtacaatgg ctaaatgagc
      841 tagggataac ggcatttggg actgacctcc ctgagtgtga tattactaat gttaatagtg
      901 ttgaagagta cctgttgaag ataaagccag atgtggtggt tcactgtgca gcctacactg
      961 cagttgacag agccgaacag gaaagggatc gctgttttgc ggtgaatgtt gaaggcacca
     1021 gaaatattgc tttatgctgt aaaaagcttg atgcaaagat gctctatgtg agcacagatt
     1081 atgtttttga tggacgtggt gattcaccgt atcatgagga tgctgaatta gcaccaataa
     1141 atttttatgg ttatacaaag gctgaagggg aaaaggtggt tcgtgatcta ttggataagt
     1201 atttcattgt gcgcacttcc tgggtatttg gcattaatgg gaataatttt gttaaaacaa
     1261 tgcttaaact tgcagaaagt aataaagaaa taaaggtggt gaacgatcaa attggttcgc
     1321 caacttactc ccgtgattta gcaaagctta tatgtgaaat gatacagaca aataaatatg
     1381 gaatatacca tgtttccaat gaaggctact gtagctggta tgaatttgct catgaaattt
     1441 tcagattagc tggaaagaag attgatgtta ttccaatatc ctcggcagag tatcctgtta
     1501 aggcaaagag gccacataat tcaagacttg caaaagataa tcttgataaa aataaattta
     1561 gtaggctacc ccattggaaa gacgcacttg cacgattttt atctgaattg aaaggagagg
     1621 tggatggtta atagcaaaat gagaatttta gt
//
LOCUS       DAORYL010000224         1625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_51111_length_1625_cov_8.769427, whole genome shotgun sequence.
ACCESSION   DAORYL010000224 DAORYL010000000
VERSION     DAORYL010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51111_length_1625_cov_8.769427"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            411..707
                     /locus_tag="PLV81_15965"
     CDS             411..707
                     /locus_tag="PLV81_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11638.1"
                     /translation="MFQRFYTYIFISFCIVTLLCSGCSHTYVIKNVSSINHEEVKGNP
                     RIALVGFYPYTSTLVDKTYSQNYTNYSTFSKQCICIRCNKCTMECEHFLALWFL"
     gene            745..1374
                     /locus_tag="PLV81_15970"
     CDS             745..1374
                     /locus_tag="PLV81_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11639.1"
                     /translation="MYVVYSDGSIKSGSPKHYLMSYGIASDYLPFKALLFNDDNSTIR
                     FGFRAKANSNSISQKITVGDGDVYKQYEWKKDYMSYYSLSGGVVVYLAPYVVNLAEHD
                     NDSMRSAPYMFTLFATVGTLLQGKITPYPIVQSEVLKTQKSYSLNGVIYETGMGFEIS
                     AATNRHIGCNVAYNVVQYNIDSSIKKDFDYKTSNLFNTINIEIYAGITF"
BASE COUNT          556 a          243 c          255 g          571 t
ORIGIN      
        1 ttatgcgctt aaagaaaaag ctgccgcatt taacctttgt tgattatgac gattacgaac
       61 gattatccag caaaaacaag gctgctgact tgtttaaaaa tatgtagtaa ttcaacaaag
      121 tgcacttcaa ctatctagta aacaaaaata tatcaatatt gttataatcc acaaattatc
      181 ccttaatgca atttaaaaaa atatagtttt tcacgctccc tatggtggca ggagtacatt
      241 catagtgtta ttcatacaat aagtattttt aaaggttaca cctttaaaat ttttttaaaa
      301 aaattaaaat ttttttcaaa tttttttaat tttttccctc ccaaagcgta tttatccata
      361 aacaccatct ggtgttaatt ctattaaata tagtttaaag gagggttttt atgtttcaaa
      421 gattttacac ttatattttt ataagctttt gtattgtaac attactatgt agcggttgct
      481 cacatacgta tgttatcaag aatgtttcat caattaatca tgaagaagta aaggggaatc
      541 cacgcatagc attagttgga ttttatcctt acacttcaac attagttgat aaaacatatt
      601 ctcaaaatta tactaattat tctaccttta gcaagcaatg tatttgcata cgatgcaata
      661 aatgcacaat ggaatgtgaa cattttctgg cactatggtt tttgtagtgg aaatacattt
      721 gatcacgaaa aagattctgc aaccatgtat gttgtatata gtgatggttc tattaaatca
      781 ggttcaccca aacactatct gatgtcgtat ggaatagctt ctgattatct gccattcaag
      841 gctctactgt tcaatgacga taactctaca atacgatttg gatttagagc aaaggctaat
      901 agtaattcaa tttcacaaaa aattacagta ggtgatggtg atgtatataa acaatatgaa
      961 tggaaaaaag attatatgag ttattattca ttatcaggag gtgtggtggt ttatttagca
     1021 ccgtatgttg ttaatcttgc agagcacgat aatgattcaa tgagatcagc gccttacatg
     1081 tttactcttt ttgcaactgt tggaacactg cttcaaggca aaataacacc atacccaata
     1141 gttcaaagcg aggttttgaa aacacaaaaa agttattcat taaatggtgt aatctatgaa
     1201 actgggatgg gttttgaaat ttccgcagcc accaacagac atataggatg taatgtcgct
     1261 tacaatgtcg ttcagtataa tattgattca tcaattaaaa aggattttga ttacaaaact
     1321 tctaacttgt ttaatactat aaatattgag atatatgcag gaataacatt ttaaatacta
     1381 ttaggggttg tctcaaaagt gcttgttgtg taaatttcgt aaatatttga ttatacatca
     1441 cactcatatc ggtacagttt tacttcactc agcagcaaaa taactgaggt gagtgctgcg
     1501 aaatttgtat cgaaacaact tttgagacaa accctgaatg caatacctta aagatatttt
     1561 tcaatatttg aaaaatctgt cttgacattt tttcttttga ttatagtatt tattgtcgat
     1621 gttag
//
LOCUS       DAORYL010000225         1576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_53449_length_1576_cov_7.648915, whole genome shotgun sequence.
ACCESSION   DAORYL010000225 DAORYL010000000
VERSION     DAORYL010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53449_length_1576_cov_7.648915"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="PLV81_15975"
     CDS             <1..959
                     /locus_tag="PLV81_15975"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOM11640.1"
                     /translation="SPQLVDDAVKAYAGKGIDVVVSIGGGSAIDAGKAISAMLPVGGS
                     VTDYLEIVGTKQHSGVKIPFIAVPTTAGTGSEATKNAVLSTTGKNGFKRSLRHDNFVP
                     TIAIIDPELMVGCPAGVTAASGLDALTQLIESYVSTQANPFTDALAESGIAKIGHSLL
                     PLCIHHEDTVFHRANMAYGAFISGITLAHAGLGVVHGLASPLGALFNIPHGVACGTML
                     AAATKATIKKLKKMGDSYYVEKYATVGELLKGYKSNSVHESCHKLIATLEEWVDILRI
                     PRLSEYGVCQDDFAAIIKDGGNKNNPAVLDEQEIMQILRERL"
BASE COUNT          439 a          286 c          369 g          482 t
ORIGIN      
        1 cttcaccgca gcttgttgat gatgcggtga aagcatatgc aggcaagggc attgatgtgg
       61 tagtgtcaat tggtggtgga agtgcaattg atgcgggtaa ggcaatttca gcaatgctac
      121 ctgtcggtgg aagtgtaact gattatctgg agatcgttgg aacaaaacag catagtggtg
      181 taaagattcc tttcattgct gtgcctacta ccgctggcac ggggagtgaa gcaaccaaaa
      241 atgctgtgct cagtacaact ggcaaaaacg ggtttaagcg ctctctgagg catgacaatt
      301 ttgtgcccac tattgctatc attgatccag agcttatggt tgggtgccct gccggagtca
      361 ctgcagcaag cggacttgat gcgcttacgc aattgattga gtcgtatgtg tcaacacagg
      421 caaatccttt caccgatgct ctggccgaaa gtggtattgc aaagattggg cacagtttgt
      481 tgccactatg tattcaccat gaagatacgg tgttccatcg cgctaatatg gcgtacggcg
      541 catttatatc gggcataacg cttgcgcatg cagggcttgg cgtggtgcat gggttggcgt
      601 ctccgcttgg tgcattgttt aatattcctc atggcgttgc ctgcgggaca atgctggctg
      661 ctgcaacaaa agctactatc aaaaaattaa aaaaaatggg cgatagttac tatgtagaaa
      721 aatatgctac agtgggagaa cttttgaaag gatataaaag caacagtgtc catgaatcgt
      781 gccataagct tattgcaacg ttagaagagt gggttgatat attgcgcata ccccgcctat
      841 ctgaatacgg tgtttgccaa gatgattttg ctgcaataat taaggatggt ggcaataaga
      901 ataaccctgc agtgcttgat gaacaggaaa taatgcagat actacgtgaa aggttgtaaa
      961 gattatggtc tgtattcaat ctcatcaaac agtaacataa taagtttatt ggtccactca
     1021 aattgatgct ttgaacgctt atggttgtac cagctcaaaa catcaggtac gtaatgtcag
     1081 cttatatcat tttttagaaa ttacaaatat tcatggatac cgtccaggag ccagcatgag
     1141 gtgtgccctc ccaggagttg agcgccccga aggaatttaa atttttccat tgttttataa
     1201 acgctaattt ttttgcttgt ttcattttgc tactgaatag ttattcgtga gcagggtggt
     1261 aacaacatat ttacgatgtt ttactgaatg tattctcagg agtttcagtc tgatacgggc
     1321 tgaacactgc ttgaggattg aatattcact ttgttatata taaattgaga ttgaagatta
     1381 tataggtgat tcccggcaat ctttaattgg gattattcat caagaactat ttataaatat
     1441 ttgtattatt gatatctata tacaggatat tgtatctaga atttgttgga cctcatccac
     1501 ggtttcacac gtcattattt tctgccttag tttgcttgca ccaggaaacc ctttaaggta
     1561 atgcacaata tgcttg
//
LOCUS       DAORYL010000226         1551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_54634_length_1551_cov_8.103610, whole genome shotgun sequence.
ACCESSION   DAORYL010000226 DAORYL010000000
VERSION     DAORYL010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54634_length_1551_cov_8.103610"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..94)
                     /locus_tag="PLV81_15980"
     CDS             complement(<1..94)
                     /locus_tag="PLV81_15980"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOM11641.1"
                     /translation="MKKFEILGTGCPKCNMLQNLTEQAAKELGLQ"
     gene            complement(275..586)
                     /locus_tag="PLV81_15985"
     CDS             complement(275..586)
                     /locus_tag="PLV81_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOM11642.1"
                     /translation="MAWLAGYPREKVNWHPTVDLEKCVKCGMCMNCGRQVYTWKKEGP
                     VVENPLSCIVGCTTCANLCKGNAITFPDIKELRALYEREKIWAHVTKQMKEEGKLTVK
                     E"
     gene            complement(632..1081)
                     /locus_tag="PLV81_15990"
     CDS             complement(632..1081)
                     /locus_tag="PLV81_15990"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOM11643.1"
                     /translation="MSNTVLVAIDFSEFSQKILDSGIKIAKGLKGKVDLIHVEEDVYR
                     IRRSHEITPMDEEFFPVIQRFHEARLTQCQKELEKLYKKIPSALRGKKIIVEGHPTEE
                     IAKYIDANKVTVLVIGSHGSSNLPISRLGSTTEKLARKASCSVLIIK"
     gene            complement(1103..>1551)
                     /locus_tag="PLV81_15995"
     CDS             complement(1103..>1551)
                     /locus_tag="PLV81_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11644.1"
                     /translation="YGMLTVNPFEHFSLIAFNRYCITNDNDRSDNYTNDTAFGAFYTQ
                     DVVKAGVVYALPVEEVSGQKTNYRLVDSYLHIDLSYVWTVPLIIAGRVGYGKNTDNDV
                     TTKIYSGGVGYRFSKDIRTIVYYQHKKDDTKSKADEVMYVKCGISF"
BASE COUNT          442 a          357 c          206 g          546 t
ORIGIN      
        1 actgcaaacc taactccttt gcagcctgtt cggttaaatt ttgcaacatg ttgcattttg
       61 ggcaaccggt acccagaatt tcaaactttt tcattactac ctcctaccta actgaatatc
      121 aatctattat ttgctattta ctaccaaaat atgtcatttt catcaatttt ctatccacaa
      181 atacatgcat gtttaatttt cacatgcttt tacaatactc ataattattc taatctactt
      241 ccagtagaca tccgaataat tcttcaacat ttaactactc ttttactgta agtttgcctt
      301 cttccttcat ttgtttggtc acatgcgccc atattttttc ccgctcatat aatgctctga
      361 gttctttaat atctgggaat gtgatagcat tccctttaca aagatttgca catgtggtgc
      421 aaccaacaat acagcttaaa ggattttcta caaccggtcc ttcctttttc catgtatata
      481 cctgccttcc acaattcatg cacatgccac acttaacaca tttttctaaa tctacagttg
      541 gatgccagtt aaccttttct cgtggatacc ctgctaacca tgccataatg acctcctgat
      601 ataaatatat ttttaaaact ctatttacac tctatttaat gatcaacacc gaacatgaag
      661 ccttgcgtgc taacttttca gttgttgaac ccaaacgtga tattggcagg ttgcttgaac
      721 catgagaacc aataaccagc actgtcacct tatttgcatc aatatatttt gcaatctctt
      781 ccgttggatg cccttccaca attatctttt ttccccgcaa tgccgatggt atttttttgt
      841 aaagcttttc aagctctttt tgacactggg tcagccgtgc ttcatgaaat ctttgtatta
      901 ccgggaaaaa ttcttcatcc attggtgtta tttcatgtga acgccttatt ctgtatacat
      961 cttcttcaac gtggataaga tccactttgc cttttaaacc tttggcaatt ttaatgccgc
     1021 tatcaagaat cttctgcgaa aactctgaaa aatctattgc aacaagaact gtattgctca
     1081 tatcattcct cccttataca cattaaaaac ttataccaca tttaacatac ataacctcat
     1141 cggctttact tttagtatca tcttttttat gctgataata cacaatagtt cgtatatctt
     1201 tgctgaagcg atacccaact ccacctgaat atatctttgt agtaacatca ttatcagtat
     1261 ttttaccata gccaacccgt cctgctataa tcagtggtac agtccacaca tacgagagat
     1321 caatatgcag gtaactatct acaagtctat agtttgtttt ttggccactc acttcctcaa
     1381 caggaagtgc atatacaaca ccagccttta caacatcctg tgtatagaat gcaccaaacg
     1441 cagtatcgtt ggtatagtta tctgatctgt cattatcatt ggttatacag tatctattga
     1501 atgctataag gctgaaatgt tcaaacggat taacggtaag cataccatag t
//
LOCUS       DAORYL010000227         1523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_56136_length_1523_cov_13.525886, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000227 DAORYL010000000
VERSION     DAORYL010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56136_length_1523_cov_13.525886"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            308..751
                     /locus_tag="PLV81_16000"
     CDS             308..751
                     /locus_tag="PLV81_16000"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOM11645.1"
                     /translation="MIHYERIPDNVYEYIRGVKQALYSDTNVIFAYLFGGLAQGVKKP
                     LSDIDIAVYVRDVNNLAEYTMNLFLTLSDICKTSEIDIVILNTAPESLAGRILQHKML
                     LIDKEPFKRHTYESVTLRKYFDFAIKEKQILYRRYKIGRYAPDFP"
     gene            723..1151
                     /locus_tag="PLV81_16005"
     CDS             723..1151
                     /locus_tag="PLV81_16005"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOM11646.1"
                     /translation="MVDTPLIFRKLSELETYYKQIKEFQHITYDTYVSDWKTQRIVER
                     TLQIMIETCTDIANHIISDEQLRTPVTYSDTFKVLCENNIIPVDLFDIMVKMVKFRNI
                     IVHQYERVDSQIVIVILKKHIDDFIYFKNAIVKYLQHTMQ"
     gene            1163..1471
                     /locus_tag="PLV81_16010"
     CDS             1163..1471
                     /locus_tag="PLV81_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11647.1"
                     /translation="MIDSSALFMLAMIGSVFMWIVLLFKIVLSPLRETVKWKLVGVLF
                     VFALCYIFVPSWVVDTFKLSGYGVPALLVNALFVGLVVMYFLITSAIKRQEKIKNAGR
                     "
BASE COUNT          497 a          224 c          291 g          511 t
ORIGIN      
        1 tgaattaaaa aacatactat aatcagggtt ttggttcatc ttatgcaatg caaagatgaa
       61 aatctattat ttacaactgc ttgaaatcat tggcgatgtg ccatagtaac tataatcatc
      121 ataacacctg gaaaattgta cattacacaa taatgatggt caaatatatt tacaaaaatt
      181 ttatagtgta tgaaatagta ttatcaacta tgtgtattgc aatgaagtgg cactatttta
      241 attgacacta aaagcgtatg gacatatctt ataaatatca tttaattaag gacatgtaac
      301 aatcaccatg attcactatg aacgcatccc tgacaatgtg tatgaataca tacggggagt
      361 taaacaggca ttatatagtg atactaatgt tatttttgct tacctgtttg gaggattggc
      421 tcagggcgtt aaaaaacctt tatcggatat tgacattgct gtgtatgtgc gtgatgtaaa
      481 caatcttgca gagtacacta tgaatctttt tttaacattg tcggatattt gtaaaacatc
      541 tgaaattgat attgttattt taaacactgc acctgaaagc cttgcgggac gcatactgca
      601 acataaaatg cttttaatag ataaggaacc tttcaaacgg catacctatg aatcagtgac
      661 attaaggaaa tattttgatt ttgctattaa agaaaagcag attctgtaca ggaggtataa
      721 gattggtaga tacgcccctg attttccgta aattgtctga gcttgaaaca tattataagc
      781 aaatcaagga attccagcat attacttatg atacctacgt gtctgattgg aaaactcaga
      841 gaatagttga acgaacattg cagataatga ttgaaacatg tactgatata gcaaatcaca
      901 taatttcaga tgaacaattg cgtacacccg ttacttattc agatacattc aaggttttat
      961 gtgaaaataa catcatacct gtggatcttt ttgatataat ggtaaaaatg gttaagttcc
     1021 gtaacattat tgtacatcag tatgagcgtg ttgattcgca aattgtaatt gtaatattaa
     1081 aaaaacatat tgatgatttt atttatttta agaatgcaat tgtgaaatat cttcaacata
     1141 ctatgcaata aaggatacta ccatgattga ttcatcagct ctctttatgt tagcaatgat
     1201 tggttcggtg tttatgtgga tagtgctgtt atttaagata gtcctcagcc ctttgcggga
     1261 aacagttaag tggaagctgg tgggtgtttt gtttgtgttt gcgctgtgtt atatatttgt
     1321 gccatcctgg gttgttgata catttaaact ttctggctat ggagtacccg cgcttctggt
     1381 aaatgctctg tttgtgggtc ttgtggtgat gtattttctc attaccagtg caatcaaacg
     1441 ccaggaaaaa ataaaaaatg cagggcgata gtaactggaa ttatgtgcac ggctgaacca
     1501 ggttgtagtt gctatgggaa cac
//
LOCUS       DAORYL010000228         1457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_59874_length_1457_cov_9.309558, whole genome shotgun sequence.
ACCESSION   DAORYL010000228 DAORYL010000000
VERSION     DAORYL010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59874_length_1457_cov_9.309558"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PLV81_16015"
     CDS             complement(<1..669)
                     /locus_tag="PLV81_16015"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOM11648.1"
                     /translation="MKSLHGVNIYITGGSSGIGLAFARLAASKGANIAIIARDTKKLE
                     QAKKEIYSVCKSSSQKVEAYSIDVCDAKKLYKAVPKMILKSGNPDVLVCAAGVGLSNY
                     FEKIADDDFKKIMDINVFGIWYTLKAFYPYLKNRNTHCVLLSSLAGLIGVYTYSAYGT
                     SKFAVFGLADCLRSEFKENGITLSVICPPEVNTPFLQEELKTIPVKAKRIKKITGVLS
                     ADTIA"
     gene            complement(813..>1457)
                     /locus_tag="PLV81_16020"
     CDS             complement(813..>1457)
                     /locus_tag="PLV81_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11649.1"
                     /translation="ASVNPQLKAMVDKQFAQSGQTTYAGTQKFMKPKPYAVGQYVVMG
                     QTDSDGKKSITRMAIVGKQQGGWIFENYTVSESQEGIMQMLIVGLEKVATTGNIDDLD
                     ILWVKIKDEKGQIQTIEGPMLSMMKGLYKKGLASVSMKINTYKDGGVIQVPAGIFKGT
                     NVVDTETTIFGKKTNTTGWYHYSVPINGIVKAVDDDGNTLVLLQFGFDAKPMMK"
BASE COUNT          425 a          334 c          213 g          485 t
ORIGIN      
        1 ggcgatagta tctgctgaaa gaacccctgt aattttttta atacgttttg cttttaccgg
       61 aattgttttt aattcctcct gcaaaaaggg tgtattcact tcaggaggac aaatcactga
      121 aagggtaatt ccattttctt tgaactcgct tcttagacaa tctgccagac caaacactgc
      181 aaactttgaa gttccatatg ctgaatacgt gtatactcca atgaggccag caagtgaaga
      241 caacagtaca caatgtgtgt ttctattttt gagatatgga taaaatgctt tcagtgtgta
      301 ccatatgcca aagacattaa tatccatgat ttttttaaaa tcatcatctg ctatcttttc
      361 aaaataatta gataaaccca caccagccgc acacaccaaa acatccggat tacccgactt
      421 cagtatcatt tttggaaccg ctttatacag cttttttgca tcacacacat ctattgaata
      481 cgcttcaact ttttgactgc tgcttttgca tacagagtaa atttcttttt ttgcctgttc
      541 caatttcttt gtatcgcgtg caataattgc aatgtttgca ccctttgaag cagctaaccg
      601 ggcaaaagcc aatccaatac cgcttgagcc accggtaata taaatattta caccatgtaa
      661 tgatttcatg tagctccctc cataaaaact atatgtattt caaaaaaaca catagctata
      721 atagataatc cccccaacat caaaaaatgc agcacaatcc tcaagccagc acataaagga
      781 cgaaagcagt ttaaatacaa tttatatggt tattatttca tcatgggctt tgcatcaaat
      841 ccaaactgta ataataccag tgtattgcca tcatcatcta cagcttttac aatgccatta
      901 atgggtactg aataatgata ccaccctgtt gtattagttt ttttaccaaa tatagttgtt
      961 tcggtatcca caacattggt tcctttaaat attcctgcag gcacctgtat gactccacca
     1021 tctttatatg tgttaatctt catgctaaca cttgccagcc ctttcttata caatcctttc
     1081 atcattgaaa gcattgggcc ttcaatggtc tggatctggc ctttttcatc cttaattttc
     1141 acccataaaa tatccagatc atcaatattg cccgttgttg caactttttc caatccaact
     1201 atcagcatct gcataatgcc ttcctgactc tctgacaccg tataattttc aaatatccac
     1261 ccgccctgtt gtttcccaac aattgccatg cgtgtgatac tttttttacc atcggaatca
     1321 gtttgtccca ttacaacata ctgcccaact gcatacggct ttggtttcat aaatttttgt
     1381 gtgccagcat acgttgtttg tcccgattga gcaaattgtt tgtcaaccat tgcctttagt
     1441 tgtggattaa ccgaagc
//
LOCUS       DAORYL010000229         1434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_61316_length_1434_cov_9.137781, whole genome shotgun sequence.
ACCESSION   DAORYL010000229 DAORYL010000000
VERSION     DAORYL010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61316_length_1434_cov_9.137781"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLV81_16025"
     CDS             <1..302
                     /locus_tag="PLV81_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11650.1"
                     /translation="KKYCDSVLASYRAIFYAIRVFLVDKDIDSDDPDRIIEIFEKYLQ
                     STLFGNTTIIEIARRSKEIKDFAVNSNGQIQYEQAEVMLNNATTILHEVERYYSH"
     gene            complement(299..709)
                     /locus_tag="PLV81_16030"
     CDS             complement(299..709)
                     /locus_tag="PLV81_16030"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HOM11651.1"
                     /translation="MKLCTLQYEPIDVDALLAQCTNPSDGAVVCFIGRPRNIDNGREV
                     LYLEYEAHQSMAIKELTTIMQKALEQFTINDCIVVHRLGRVEVGQASVVIIVTAMHRK
                     EAFNASQFIIDELKKSVPIWKKEYFNDGSQWKSG"
     gene            complement(717..962)
                     /locus_tag="PLV81_16035"
     CDS             complement(717..962)
                     /locus_tag="PLV81_16035"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOM11652.1"
                     /translation="MKIIVQAFAEAAEVLGFRKKQFDVENDITASELVRLLAHSNQEF
                     KRIAKTLFVAINEEYCSPDAVIHENDVVDLIPPVGGG"
     gene            complement(969..1259)
                     /locus_tag="PLV81_16040"
     CDS             complement(969..1259)
                     /locus_tag="PLV81_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11653.1"
                     /translation="MPLKKDSKKPQSKGKRKTNLLKLCTVMNKLSVDAVDKEVVKRFR
                     TVIDSAEEDLTKSLVNQIINHSDELELTAVPEMFHPYVKHYLFMKKRNTKKK"
BASE COUNT          462 a          260 c          218 g          494 t
ORIGIN      
        1 aaaagaaata ttgtgattcc gttttagctt cttaccgggc aattttttat gctatccgtg
       61 tgtttttagt tgataaagat attgattcag atgatccaga taggattatt gaaatatttg
      121 aaaaatattt acaatcaaca ttatttggga atacaactat tattgaaatt gcaagacggt
      181 caaaagagat aaaggatttt gctgttaact caaatggtca gattcagtat gaacaggctg
      241 aagtgatgct taacaatgca acaacaattt tacatgaagt tgaacggtat tactcacatt
      301 aaccactttt ccattgtgaa ccatcattga aatattcttt tttccatata ggaacacttt
      361 tctttaattc atcaataata aactgagatg cattaaacgc ttctttacga tgcatggcag
      421 taacaataat tactacactt gcctgaccaa cttccacacg gcctaaccga tgtaccacga
      481 tgcaatcatt aatggtaaat tgttctaaag ccttctgcat aattgttgta agttctttta
      541 ttgccatgga ttgatgcgct tcatattcta agtataatac ttccctacca ttatcaatat
      601 tccgcggcct gccaataaaa catactaccg ctccatcgga aggattggtg cattgtgcaa
      661 gcaatgcatc cacatcaatt ggttcatatt gtaacgtaca cagcttcata aaagaatcaa
      721 ccccctccta cgggaggaat aaggtcaaca acatcatttt catgaattac cgcatcaggg
      781 gaacaatact cttcatttat tgctacaaat aaagtttttg ctatacgctt aaattcctga
      841 tttgaatgcg ctaaaaggcg caccagttca gaggctgtta tatcattttc aacatcaaat
      901 tgtttttttc tgaaacctaa tacctcagct gcttctgcaa atgcctgaac tataattttc
      961 ataatttttt atttcttctt ggtgtttcgt tttttcataa aaaggtagtg tttcacataa
     1021 gggtgaaaca tctctggcac tgctgtcagt tctaattcat cagagtgatt aataatctga
     1081 ttcacaaggc tttttgtcaa atcctcttca gcagaatcta ttacagtcct gaaacgtttt
     1141 accacttctt tgtcaacagc atcaaccgaa agtttattca taactgtaca taatttcaaa
     1201 agatttgttt ttcttttgcc ttttgattgg ggcttttttg aatccttctt caatggcatg
     1261 agaaaattac ctcccaaaaa ataatctgca atgttaaaag tattctatat tatgtaaaga
     1321 ttaatttcaa aaaaatataa acaaacgtaa tctaaaaata ttcttttacc gaaatatgta
     1381 gtgaatcata ctttttgcag gagaaacgtt tatgaaatgt gataaatgtg gttg
//
LOCUS       DAORYL010000230         1431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_61525_length_1431_cov_3.300145, whole genome shotgun sequence.
ACCESSION   DAORYL010000230 DAORYL010000000
VERSION     DAORYL010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61525_length_1431_cov_3.300145"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..1350
                     /gene="rfbB"
                     /locus_tag="PLV81_16045"
     CDS             274..1350
                     /gene="rfbB"
                     /locus_tag="PLV81_16045"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOM11654.1"
                     /translation="MNVNTNFKCILVTGGAGFIGSNFIRYLFEKVRYDGKIVNLDKLT
                     YAGNLENLVEIDNRFGGKQYFFEKADICDFDAVNAIFSNHRPDCVVHFAAESHVDRSI
                     HGPKDFIYSNIIGTFNLLEACRKWWDDSFYRFHHISTDEVYGSLGVTGYFYETTAYDP
                     RSPYSASKAASDHLVSAYHHTYGLNATISNCSNNYGPYQFPEKLIPLMILNMLEEKPL
                     PVYGDGKNVRDWLYVEDHCSGVWLVVNNGTEGETYNIGGENEWHNIDLVKRLCEIVAV
                     KKGKDTDYYKKLITFVNDRPGHDRRYAINCDKIKHELGWKQSVTFDEGLDKTVEWYLH
                     NQKWIENVKSGQYMKWLEMQYNKR"
BASE COUNT          441 a          201 c          328 g          461 t
ORIGIN      
        1 aaaaagtcgt tggtgaaaat atcccttcct ggcaaagtgg gattgataga tggcttgagg
       61 aaaggaatgg ggagttctag gttcaatgtt caatgttcaa tgttcaatgt tatacaattt
      121 cattttttta gttttgatct tgaagtgaaa tgtaatgtaa tgtaatgtaa tgtaatgtaa
      181 tgaataaagc acattgttta tgtttattac tttttaaact ttgaaccttg aacgtagatc
      241 ttttttaaaa tacaattatg ggtatagcgc tttatgaatg taaacaccaa ttttaaatgc
      301 attctggtaa cgggtggtgc aggctttatt ggatccaatt ttattcggta tctatttgaa
      361 aaagtaaggt atgatggaaa aattgtaaat cttgataaac tcacgtatgc tgggaattta
      421 gaaaatttag ttgaaattga taatcggttt ggtggcaagc agtacttttt tgaaaaggct
      481 gatatctgtg attttgacgc agtgaatgca attttcagta accatcgccc tgattgtgtg
      541 gtccattttg ctgcagaaag ccatgttgac cgttccattc atggaccaaa ggattttatt
      601 tattctaaca ttattggaac ttttaacctg cttgaagcct gtcgtaagtg gtgggatgat
      661 tctttttatc gctttcacca tatcagtact gatgaagtgt acggttcact tggtgtaact
      721 ggctactttt atgaaacaac ggcgtatgat ccgcgcagcc catattctgc atcaaaagct
      781 gcatctgatc atctggtgag tgcataccat catacgtatg gtttgaatgc gactatctcc
      841 aattgctcaa acaattatgg gccctatcag tttcctgaaa agctcattcc acttatgatt
      901 ttaaatatgc ttgaagaaaa accattgcct gtgtatggtg atggaaaaaa cgtgcgtgac
      961 tggctgtatg tggaagatca ctgctcaggg gtgtggcttg tggtgaataa tggtacagaa
     1021 ggggaaacat ataatatcgg tggcgaaaat gaatggcata acattgacct ggtgaagagg
     1081 ttatgtgaga tagtagctgt aaaaaaaggg aaagatactg attattataa aaagcttatt
     1141 acctttgtaa acgataggcc cggccacgac agacgctatg ctatcaactg cgataaaatt
     1201 aaacatgagc ttggttggaa gcagagtgtg acttttgatg agggacttga taaaaccgtg
     1261 gagtggtatt tgcataatca aaagtggatt gaaaatgtaa aatctgggca atatatgaaa
     1321 tggcttgaga tgcagtataa taaaaggtag gagatcaacg ttcaatgttc aacgttcaat
     1381 gtttgggtat gtacgatgaa aattgaaaga tttgaggata ttgatgcctg g
//
LOCUS       DAORYL010000231         1417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_62405_length_1417_cov_11.706314, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000231 DAORYL010000000
VERSION     DAORYL010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62405_length_1417_cov_11.706314"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1371
                     /locus_tag="PLV81_16050"
     CDS             <1..1371
                     /locus_tag="PLV81_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11655.1"
                     /translation="QQAESLVIEGQVMLCGEGMEGVCVQMQHPEYGTVETYTDSMGIY
                     RFEQAWVGEYSIMPYVEHCEIVPVQRKLNVTDNTVVNDFVVRPLWEKVYGSDYHDITM
                     DMAKTLRCGFVYTGKTDYTGSYGIFLMRVDALGQQLWYKEYAQNWYMSANSVVALDDG
                     SVVVTGCKADSINSGNIWIARFDGNGDSIWEKIYSGSLWDEGHGIVRIESGSDTGFII
                     AATTESAGSGRADVYIMKVDNNGNIVGNLFDALFGGQYNDGVTRIIASKDGGYIFTGF
                     VEYETLVSADSRITKINSMRQHVWSKEYNIDTYDYATDVVELDNGDIIVCGIAYETLN
                     RDRIWIARLTGDGNTEVWKKEFSIGRSSGVYGMTLDATGNIICTGYTIPENEGVSKLL
                     LLSLKQNGEFLWMQTYGSIAGTGYSGRRVVCAGDNGLAVAGTTIESGTGADVYCLKVN
                     DNGELW"
BASE COUNT          431 a          185 c          397 g          404 t
ORIGIN      
        1 cagcaagcag aaagcctggt tattgaaggt caggttatgc tgtgtgggga aggaatggaa
       61 ggcgtgtgtg tacagatgca acatcctgaa tatggaacgg tggaaacgta tactgattca
      121 atgggaattt accgctttga acaggcatgg gttggtgagt acagtattat gccgtatgta
      181 gagcattgtg agatagttcc tgtgcaaagg aaactcaatg taactgataa cactgttgta
      241 aatgattttg tcgtgcgacc tttatgggaa aaggtgtacg gcagtgatta ccatgatatc
      301 acaatggata tggcaaaaac gctgcgatgt ggatttgtat ataccggtaa aaccgattac
      361 acaggcagtt atgggatatt tttaatgcgt gttgatgcat tggggcaaca actatggtat
      421 aaggaatatg cgcaaaattg gtatatgagt gcaaacagtg tggtggcact tgatgatggg
      481 agtgttgttg ttaccggttg taaagcagac agcataaata gtgggaatat atggattgcc
      541 cgatttgatg gtaatggtga cagtatatgg gaaaaaatat acagtgggag tctatgggat
      601 gagggacatg gtattgtacg tattgagagc ggtagtgata caggatttat cattgctgca
      661 actactgaat cggcaggaag tggccgggca gatgtatata ttatgaaagt tgataacaat
      721 ggaaatattg tcgggaacct ttttgatgca ttgtttggtg gtcaatataa tgatggagtt
      781 acgcggatta ttgccagcaa ggatggtgga tatattttta ccggatttgt agaatatgaa
      841 acactggtaa gtgctgatag tagaattaca aaaattaata gcatgcgcca gcatgtgtgg
      901 agtaaggaat ataatataga tacgtatgat tatgcaactg atgtggtgga actggataat
      961 ggcgacatta tagtgtgcgg cattgcatat gaaaccttga acagggatcg gatatggata
     1021 gctcgtttga ccggtgatgg taacactgaa gtgtggaaaa aagaattcag tattggccgc
     1081 tcaagcggtg tttacggcat gacgctggat gctaccggca acataatatg tactggttat
     1141 acaatacctg agaatgaagg tgtcagcaag ctgttgcttt tgtcactaaa acaaaatggt
     1201 gaatttttgt ggatgcaaac ctatggaagt attgcaggaa cagggtacag tggcagaagg
     1261 gtggtttgtg caggggataa tggccttgct gttgccggta ctaccattga aagtggaaca
     1321 ggtgccgatg tgtattgttt aaaggtaaat gataacggtg agttgtggta ataattgtta
     1381 aaccaattgt gaacaatcat acataattca ttactat
//
LOCUS       DAORYL010000232         1415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_62579_length_1415_cov_2.731618, whole genome shotgun sequence.
ACCESSION   DAORYL010000232 DAORYL010000000
VERSION     DAORYL010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62579_length_1415_cov_2.731618"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="PLV81_16055"
     CDS             <1..748
                     /locus_tag="PLV81_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11656.1"
                     /translation="MSYPATIGWHAGLLASQEYAVWERYGDFTVTIAKPYAGIGIFSN
                     IGVFDISVFLYGVGTYYRVSEQHAVPLHNTYNNAGLQLNFRATDDRSIAKKNKALNAT
                     LFAGVSQDENVLMSRASVYIPLQLTMRLYVVPSCYSFYTNSLNRIYAEYVFDSYLLGY
                     SKRYTATHSRHIAKLELMYELLYEFVYFEIFASSGVYKSEYVWWDRASSYGAGADVYY
                     NTILFTIGCAWNTDEQFGEYYIYSGIKSSW"
     gene            complement(818..>1415)
                     /locus_tag="PLV81_16060"
     CDS             complement(818..>1415)
                     /locus_tag="PLV81_16060"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOM11657.1"
                     /translation="TGLTLFNNGLVDVAWSHLYDNETESYNIPFVKKLCTNRELVVVH
                     LFDREVGLIIAPDIKHIIKDFSSINQPEVVFVNRQEGSGIRQLIDLYIARHAINPHHI
                     NGYTNVKYSHIETGLAIQSGEANCGVASVAIANFFKLPFIPLTVESFDMIISKEIFFN
                     QTIQNLIKLLHDQPFINKIKSIGNYNFFNSGEIIYVTS"
BASE COUNT          443 a          222 c          272 g          478 t
ORIGIN      
        1 tatgagctat cctgcaacaa ttggatggca tgctggatta cttgcatcac aggaatatgc
       61 agtgtgggag cgctatggcg attttacagt aactatcgcc aaaccatatg ctggtattgg
      121 gatattcagc aacattggtg tttttgatat ttcggtattt ctttatgggg ttggaacata
      181 ctaccgggtg tcagagcaac atgcagtgcc cctgcataat acgtacaaca atgcaggact
      241 acaactcaat ttccgtgcaa ctgatgaccg ttccattgca aaaaaaaata aggcgttgaa
      301 tgcaacacta tttgcaggtg tatcacagga tgaaaatgtg ctcatgtcac gtgcttcagt
      361 gtatatacca ttacagctga ctatgcgtct gtatgttgtt ccttcctgtt atagctttta
      421 cacaaattca cttaacagaa tttatgcaga atatgtgttc gatagttacc tgttagggta
      481 ttcaaagcga tatactgcca cacattcacg gcatattgct aagcttgaat tgatgtatga
      541 attgctctat gaatttgtgt actttgaaat ttttgcatca tcaggtgttt ataaatcaga
      601 atatgtatgg tgggatagag catcaagcta tggtgcgggt gctgatgtat attataatac
      661 aatactgttt acaattggat gtgcatggaa tactgatgaa cagttcggtg agtattatat
      721 ctattctggc ataaaatcga gctggtaatg ctaaagtaaa aaataggttt ttcatggagt
      781 gataaaaata ttcttacaaa tggttgtatg attattttca tgatgttacg taaataatct
      841 cgccagaatt aaagaaatta taattaccaa ttgatttaat tttattgata aatggttgat
      901 catgtaggag ttttataagg ttctgaattg tttggttaaa aaatatttct ttggatatta
      961 tcatatcaaa actttcaaca gttaaaggga taaaaggtaa tttaaagaaa tttgcaatag
     1021 caacagaagc tactccgcaa ttagcttcac ctgattgtat tgccagccct gtttcaatat
     1081 gggaatattt aacattggta tatccattaa tgtgatgagg attgatggca tgtcttgcta
     1141 tatatagatc aatcaattgt cgtatgcctg aaccttcctg tcggtttaca aatactactt
     1201 ctggttgatt aatagaacta aaatctttaa tgatatgttt tatatcagga gcaatgatga
     1261 gccccacttc tctgtcaaat aaatgcacaa cgactaattc tctattggta cataattttt
     1321 ttacaaatgg aatgttatag ctttcagttt cattatcata gagatgtgac caagcaacat
     1381 ctaccaggcc attattaaat aaagttaacc cggtg
//
LOCUS       DAORYL010000233         1391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_64054_length_1391_cov_6.485030, whole genome shotgun sequence.
ACCESSION   DAORYL010000233 DAORYL010000000
VERSION     DAORYL010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64054_length_1391_cov_6.485030"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..552
                     /locus_tag="PLV81_16065"
     CDS             241..552
                     /locus_tag="PLV81_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11658.1"
                     /translation="MHYYLGYFIVILFCSVFTVLSAITGSWVLWILWDSRSFESKELW
                     MSLGVSLLFFVICFVMTKAFLKEIINSDFIGYLKRPQNYCISEGKIEKAEYIPGSGGW
                     I"
     gene            560..811
                     /locus_tag="PLV81_16070"
     CDS             560..811
                     /locus_tag="PLV81_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11659.1"
                     /translation="MIIHGTFSDNGMFIEEFQPSLWSKYFADKYEEQNLKEGDDWYSE
                     KGKRIPLPIPVWVLHKKSNLQYGSLVGIDKNIFHGKKIR"
     gene            857..>1391
                     /locus_tag="PLV81_16075"
     CDS             857..>1391
                     /locus_tag="PLV81_16075"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOM11660.1"
                     /translation="MKTRINISLFFLGLGGQIAWAIENQFFNTFLYDRIIPDPRYVSI
                     MVALSAITATITAIVMGAFSDSLGRRKPFLLYGYLAWSVSIWVIPMAEWIKPVLLAAW
                     VVIFLDSLMTFFGSTSYDAVYNAYLTDVTNESNRGKAQGLLSLATWLALLLVYGTSGP
                     IVEKCGYFIFFGIAGALV"
BASE COUNT          413 a          232 c          271 g          475 t
ORIGIN      
        1 atcttttata acaattaatt tttccaggag ttttgatttt ttcagtagac cctaagtatc
       61 aataaataac tttcttttgg ttgcacatac ttttatattg ctttattaca tcagtagtgt
      121 atgatttagt accattaaaa tatttataag aggaaaaacc caatgtataa aacatttaaa
      181 tatttggcag tggataccca atcacaacta tatgatcaaa aaagaattaa aagaatcttt
      241 atgcactact acttgggtta ttttattgtt attttgtttt gctcagtttt tactgttctt
      301 tccgctatta ctggtagctg ggtattgtgg atattgtggg attccaggtc ttttgaaagt
      361 aaagaactat ggatgtcttt aggagtaagc ctattgtttt ttgtaatatg ctttgtaatg
      421 actaaagcgt ttttaaaaga aataattaac tctgacttca ttggctatct caaacgccct
      481 caaaattact gcatatcaga aggaaaaata gaaaaagcag aatatatacc cggaagtggg
      541 gggtggatct aaaggaagaa tgattatcca tggaactttc agtgataacg gcatgtttat
      601 tgaagaattt cagccttctt tatggtcaaa atactttgct gacaaatatg aagaacaaaa
      661 tcttaaagaa ggcgatgact ggtactcaga aaaggggaaa agaataccat tgccgatacc
      721 tgtatgggta ctgcataaaa aatctaatct tcaatacggt tcgcttgttg gtattgataa
      781 aaacatattt catggtaaaa aaatacggtg atagttcact atacatacta cactttcaaa
      841 tattttggag acaaacatga aaacccgtat caatatttca ttgttttttc ttgggttggg
      901 aggacagata gcatgggcta tcgaaaatca gttttttaac acattcctgt atgaccgcat
      961 aattcctgac ccccggtatg tttcaattat ggtggctctt tctgccatca ctgccactat
     1021 cacagcaatt gtcatggggg ctttttctga ttcacttggc aggcgcaaac cattcttact
     1081 ctacggttat ttagcgtgga gcgtttctat ctgggtaata cctatggcag agtggatcaa
     1141 acctgttctg ctggctgcat gggttgtaat attcttagat tcgctcatga cgttttttgg
     1201 atcaacatcg tatgatgcgg tgtacaatgc ctacctgact gatgtcacta atgagagtaa
     1261 ccgtggaaaa gctcaagggc tactatcgct tgcaacctgg cttgcacttt tgcttgtgta
     1321 cggtacttca ggcccaattg ttgaaaaatg tggctatttt attttttttg gcattgctgg
     1381 tgcactggtg c
//
LOCUS       DAORYL010000234         1369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_65600_length_1369_cov_7.699391, whole genome shotgun sequence.
ACCESSION   DAORYL010000234 DAORYL010000000
VERSION     DAORYL010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65600_length_1369_cov_7.699391"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PLV81_16080"
     CDS             complement(<1..775)
                     /locus_tag="PLV81_16080"
                     /inference="COORDINATES: protein motif:HMM:NF040068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOM11661.1"
                     /translation="MAIDKEKIFNLVAHVSKPARYVGGEWNAVVKPDATVRVGLCFPD
                     LYEVGMSNNGIQILYSAVNALKGAACERVFAVERDFEKVLKESGTPLYTLETFTPLHE
                     LDMVGFNAACELLYTNILQVLELGNIPFFAKDRGDSYPLIVIGGEAVSNPAPLLPFAD
                     AVFAGDGEEAIVEIVNLLIEKKTKGLSKDEVLAKLANIEGVLCSRDVEFTYEGHTLKA
                     IKAPDVYKRVAHNFHGYEATRPVVPSIRSVQDRVSIQLDR"
     gene            complement(780..>1369)
                     /locus_tag="PLV81_16085"
     CDS             complement(780..>1369)
                     /locus_tag="PLV81_16085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HOM11662.1"
                     /translation="EQFAAMTQVTIVHDDFLKVAPVDECTRIVGNLPYNVSSQIIFRV
                     AKHYTALQCFFLIQKEMAARLIALPGSAMYSAFTVGVQLYFDVHKLFDIPPHAFYPAP
                     KVHSTFVKLIRKKDIPLQDNEKEIFEQLVATAFWARRKMLKTALKRSPYGTYDESFIE
                     SAFDALGFDPKVRGEELSLQDFIALAQYCAHHDKR"
BASE COUNT          382 a          336 c          257 g          394 t
ORIGIN      
        1 ctctgtctaa ctgaatggaa accctgtcct gcacactgcg tatggatggc accactggtc
       61 gtgtagcttc atatccatga aaattgtgtg caactctttt atacacgtct ggtgctttaa
      121 ttgcttttaa tgtatgacct tcataggtga actcaacatc gcggctgcat aacacccctt
      181 cgatatttgc caactttgca agcacctcat cttttgataa accttttgtc tttttttcaa
      241 taagaaggtt tacaatttcc actatggcct cttcaccatc accggcaaag actgcatcag
      301 caaatggcaa tagtggtgct ggattgctta ctgcttcccc gccaatgact attaacggat
      361 aactatcgcc cctgtccttt gcaaaaaatg ggatgttgcc cagttccagc acctgaagta
      421 tattggtgta cagtaattca catgctgcat tgaagcctac catgtcaagc tcatgcagag
      481 gtgtaaatgt ttcaagagtg tacagtggtg tccccgattc tttcaaaact ttttcaaaat
      541 cacgctccac agcaaacaca cgttcacatg ctgcaccttt cagtgcattg actgcactgt
      601 acaaaatctg aataccgtta ttggacatgc caacctcata caggtcgggg aaacatagcc
      661 caaccctgac tgttgcatca ggctttacaa cggcattcca ttcaccacct acataccgtg
      721 caggttttga aacatgcgcc acaaggttaa atatcttttc tttatcaatt gccatgctgc
      781 taccttttat catggtgtgc acagtactgt gccagtgcaa taaaatcctg taatgaaagc
      841 tcttcaccac gtactttagg atcaaagccc aatgcgtcaa acgcactttc aataaatgat
      901 tcatcatacg taccatacgg tgagcgtttc aacgctgttt ttaacatctt gcggcgtgcc
      961 cagaaagctg ttgcaacaag ttgttcgaat atttcttttt cattatcctg caaggggata
     1021 tctttttttc gtatcaactt tacaaacgtt gaatgcacct ttggcgcagg ataaaatgca
     1081 tgtggcggta tatcaaaaag cttatgcaca tcaaagtaaa gctgcacacc caccgtgaat
     1141 gctgaataca tggcgcttcc gggcaacgca atcagtcgcg ctgccatctc cttctggatc
     1201 agaaaaaaac attgcaaagc agtatagtgt tttgccaccc tgaaaataat ctgtgatgac
     1261 acattgtaag gaagattgcc aacaatacgc gtgcactcat caacaggggc taccttcaaa
     1321 aaatcatcat gtacgatagt tacctgcgtc attgcagcaa actgttcgg
//
LOCUS       DAORYL010000235         1351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_66885_length_1351_cov_7.805556, whole genome shotgun sequence.
ACCESSION   DAORYL010000235 DAORYL010000000
VERSION     DAORYL010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66885_length_1351_cov_7.805556"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PLV81_16090"
     CDS             complement(<1..775)
                     /locus_tag="PLV81_16090"
                     /inference="COORDINATES: protein motif:HMM:NF040068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOM11663.1"
                     /translation="MTIEKEHIFRLVSQVSKPARYVGGEWNAVVKPPATVRIGLCFPD
                     LYEVGMSNNGIQILYRAVNALEGAACERVFAVERDFEKILKESNTPLYTLETYTPLAE
                     LDMLGFNAACELLYTNILQVLDLGNIPFFAKDRGDSYPLIVIGGEAASNPVPLLPFAD
                     AVFAGDGEEAIVEIVQILIEKKQKQLTKDEVLTRLAKIEGVLCSRDVTFTYDGYTLTG
                     ITAPTVYKRTARNFHAFEVTRPVVPSIRSVQDRVSIQLDR"
     gene            complement(776..>1351)
                     /gene="rsmA"
                     /locus_tag="PLV81_16095"
     CDS             complement(776..>1351)
                     /gene="rsmA"
                     /locus_tag="PLV81_16095"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOM11664.1"
                     /translation="QVTIVHDDFLKLPAVQECTKVVGNLPYNVSSQIIFKVAKYYTVS
                     QCFFLIQKEMAERLIAKPGSKNYSAFTVGVQLHFEVHRLFDVPPSAFYPPPKVHSTFV
                     QLTRKKELPLTESQITLFEQLVATAFWARRKMLKTALKRSPYITFDELCIAQAFEALG
                     FDVKVRGEELSLDDFIALTKYFHHHQHVKDQ"
BASE COUNT          376 a          309 c          267 g          399 t
ORIGIN      
        1 ctctgtctaa ctgaatggaa accctgtcct gcacactgcg tatggatggc accaccggcc
       61 gagtaacttc aaatgcatgg aaattgcgag cagtcctttt atacactgtt ggtgcagtaa
      121 tgccggttaa cgtatatcca tcgtatgtaa atgtaacatc gcgactgcac agtactcctt
      181 ctatctttgc aaggcgggtt aacacttcat cctttgttaa ctgtttttgc tttttctcaa
      241 tgagtatctg cacaatttcc actatagctt cttcgccatc gccggcaaag actgcatcag
      301 caaagggtaa aagtggcacc gggttgcttg ctgcctcccc accaatgact attaacgggt
      361 aactatcgcc cctgtccttt gcaaaaaatg gaatgttgcc taaatccaat acttgaagta
      421 tattggtgta taataattcg caggctgcat taaagccaag catatcaagc tcagctaacg
      481 gcgtatatgt ttcaagtgtg tacagtggtg tattagattc tttcaaaatc ttttcaaaat
      541 cgcgctccac agcaaacacg cgctcacagg ctgcaccttc tagcgcatta accgcacggt
      601 acaaaatctg aataccgtta ttggacatgc caacctcata caggtcaggg aaacacagtc
      661 caattcttac tgttgctgga ggttttacca cagcgttcca ttcaccgccc acatagcgtg
      721 caggttttga aacctgtgaa acaagcctga atatatgttc tttctcaatt gtcatttatt
      781 gatccttcac gtgttgatga tgatggaagt atttcgtcaa tgcaataaaa tcgtcaagtg
      841 aaagctcctc accacgcact tttacgtcaa agccaagcgc ttcaaatgcc tgcgcaatac
      901 acaactcatc aaacgtaata tacggtgagc gtttcaatgc agtttttaac atcttacggc
      961 gtgcccagaa ggctgttgca acaagctgtt caaataatgt tatctggctt tctgtcaggg
     1021 gcaattcttt tttccgtgtc agttgtacaa acgttgagtg cacctttggt ggtggataaa
     1081 atgcactggg tggtacatca aaaagcctgt gtacttcaaa atgaagctgc acaccaacgg
     1141 taaaagcgga ataatttttg cttcctggtt ttgcaatcag gcgctctgcc atctcttttt
     1201 gtataaggaa aaaacattgt gaaactgtat agtattttgc cactttaaat ataatctgtg
     1261 atgacacgtt atagggaaga ttgcccacca ctttagtaca ttcttgcact gcaggtaatt
     1321 tcaaaaaatc atcatgtacg atagttacct g
//
LOCUS       DAORYL010000236         1342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_67545_length_1342_cov_4.111888, whole genome shotgun sequence.
ACCESSION   DAORYL010000236 DAORYL010000000
VERSION     DAORYL010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67545_length_1342_cov_4.111888"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="PLV81_16100"
     CDS             <1..1034
                     /locus_tag="PLV81_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOM11665.1"
                     /translation="QCPHCGGKAKRETDTMDTFVDSSWYFAKFTSPHSKEIFDFDEVD
                     YWTPVDQYIGGIEHACMHLLYARFFTMVLNDLGILKYREPFTRLLTQGMVIKDGAKMS
                     KSKGNIVDPDDIIDKYGADTVRLFMLFAAPPEKDLDWSDKGVEGCYRFINRVWRIVQK
                     YSDCYTEYNFDNVVLDPALKNLRGETHRSVKAVTHDIENRMQFNTAIARMMELVNALY
                     QVNEEIIRTDAGKMVVSELFDKLLPMLGPFVPHVAEELWEALGHKNFILDEPWPNFND
                     ELTVKDEIEVVFQINGKIRSKRNVPADITKEEMEKVALDDDRIKEMIAGKTVKKVIVV
                     TGKLVNIVI"
     gene            complement(1035..>1342)
                     /locus_tag="PLV81_16105"
     CDS             complement(1035..>1342)
                     /locus_tag="PLV81_16105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11666.1"
                     /translation="QLWIKISSSRLYFLYGIVLILVGFPLTQVPPSNPLSTPLFVIGL
                     IVVFSGPFILIYPEKIKNIFFQGLEELGQTGSRFALIIDAIARIVTGILCIAAYMLN"
BASE COUNT          451 a          206 c          292 g          393 t
ORIGIN      
        1 ctcaatgtcc gcactgtggg ggaaaagcaa agcgtgaaac tgataccatg gatacgtttg
       61 ttgattcttc atggtatttt gcaaagttta cttcgcctca ttcaaaggaa atttttgatt
      121 ttgatgaggt agattactgg actccagttg accagtacat tggcggtatt gagcatgcat
      181 gtatgcattt gctatatgcg cgctttttta ctatggtcct gaacgatctt ggtattctca
      241 aataccgtga gccgtttact cgtttgctga cacagggaat ggtgataaaa gatggggcaa
      301 agatgagtaa atccaaaggc aatattgttg atcctgatga tatcatagat aaatatggag
      361 ctgatactgt acgattattc atgctttttg ctgctccacc tgaaaaggat cttgactggt
      421 cggacaaagg ggtagaaggg tgctatcgct ttattaaccg tgtatggcgt atcgttcaaa
      481 aatacagtga ttgttatact gaatataatt tcgataatgt tgtattggat cctgcattaa
      541 aaaatctcag aggtgaaaca cacagaagtg taaaagcagt gacacatgat attgaaaaca
      601 gaatgcagtt taatactgca attgccagaa tgatggagct ggtaaatgca ttgtatcaag
      661 taaacgaaga aataattcga acagatgctg gaaaaatggt agtatctgaa ttgtttgata
      721 aactattgcc aatgcttgga ccgtttgtgc ctcatgttgc tgaagaattg tgggaagcgc
      781 tgggtcataa gaattttatt ttggatgagc cttggcctaa tttcaacgat gaactcactg
      841 taaaggatga gatagaagtt gtgtttcaga taaatggcaa aataaggtca aaacgaaatg
      901 tccctgctga tattacaaaa gaggaaatgg aaaaggtggc acttgatgat gacagaatta
      961 aagaaatgat agcagggaaa acagttaaaa aagtaatagt tgttacaggg aaactggtca
     1021 acatagtaat ttaattaatt aagcatatac gctgcaatgc atagtattcc ggttacaatc
     1081 cgagctattg catcaataat aagggcaaaa cggctccctg tttgtcccag ttcctcaagc
     1141 ccctgaaaaa atatattctt aatcttttca gggtatatca gaataaatgg tccagaaaac
     1201 actactatta atcctataac aaaaagtggg gtagataaag gatttgatgg tggtacctga
     1261 gttaaaggaa atcccactaa tattaataca attccataaa gaaaatacaa cctgcttgag
     1321 gaaattttaa tccataattg ta
//
LOCUS       DAORYL010000237         1336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_68008_length_1336_cov_9.823575, whole genome shotgun sequence.
ACCESSION   DAORYL010000237 DAORYL010000000
VERSION     DAORYL010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68008_length_1336_cov_9.823575"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1300)
                     /locus_tag="PLV81_16110"
     CDS             complement(<1..1300)
                     /locus_tag="PLV81_16110"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOM11667.1"
                     /translation="MYQLLFTPITINTLEIKNRIAYPALGLLYSYDGTLNDRYYEYFR
                     EKAKGGAGLVTVGPVGIDIAGSGRIALSLASDDRIPDFAKLAQIIKNEGARTFVQLFH
                     AGAYSYSMLTDGIQPVAPSAVYSKYSKQTPREMTLEDIEIVQKAYVDAALRVKEAGFD
                     GVEIIASAGYLITQFLSPITNKRTDKYGGSFENRVRFPREVIEKVRQAVGKDFPISIR
                     MAGNDFVPGSNTSRETPLFAKVYEEAGVDIINVTGGWHEAKVPQLPMELPRAGYAYLA
                     ANIKKAVNVPVMASNRISSPVEAERILRDSMADMVNLGRVLIADPYWPKKAMQGKAKL
                     IRPCVGCNQGCTDTLFSGMPVGCILNACAGYEFERSIAKTSHPLKIMVVGAGPAGLEA
                     AVRAKEAGHEVEIFEKEHEIGGQLFIAAAPPNKQELLEIIR"
BASE COUNT          356 a          341 c          238 g          401 t
ORIGIN      
        1 aacggataat ctccagtaat tcctgcttat tgggtggtgc tgcagctata aacaactgac
       61 caccaatttc atgttctttt tcaaaaattt caacttcatg tcctgcctct tttgcccgta
      121 ctgctgcttc aagcccagca ggtcctgcac ccacaaccat aatcttcaac ggatgagaag
      181 tttttgcaat agagcgttca aactcatacc ccgcgcatgc atttaaaata catcctactg
      241 gcataccgct gaataatgta tcagtgcaac cctgattaca acccacgcat ggccgtatca
      301 attttgcttt tccctgcata gctttctttg gccaatacgg atcagcaatc agtactcttc
      361 ccaggttgac catatcagcc atactatcgc gaagtatcct ctcagcctca acaggtgagc
      421 tgattctgtt ggaagccatt actggcacat ttactgcctt tttaatattg gcagcaagat
      481 atgcataccc tgcacgtggc aattccatgg gcagttgtgg aaccttagct tcatgccatc
      541 caccggtaac attaatgata tccacacctg cttcttcata tactttagca aacaacggtg
      601 tttctctgct tgtgttactt cccggaacaa aatcattccc cgccatccgt atgcttatgg
      661 ggaaatcctt tcccactgcc tgcctaactt tttcaatcac ctctcgaggg aagcgcaccc
      721 tgttttcaaa tgagccgcca tatttgtctg ttcgcttatt tgttatgggc gatagaaact
      781 gtgtaatgag ataccctgca gaagcaataa tctcaacgcc gtcaaagcca gcttccttta
      841 cacgcagcgc agcatcaaca tatgctttct ggacaatttc tatatcctcc agtgtcattt
      901 ccctgggtgt ctgttttgag tattttgaat acaccgctga aggagcaaca ggctgtatgc
      961 catctgtgag cattgaatag gaataagccc cagcatgaaa cagttgcaca aatgtccgcg
     1021 caccttcatt ttttattatc tgtgccaatt ttgcaaaatc aggaatcctg tcatctgatg
     1081 ccagtgataa ggcaatcctg ccagatcctg caatatctat tccaaccggc cccacagtaa
     1141 caaggcctgc accacctttt gctttttccc tgaaatattc atagtatcga tcattcaaag
     1201 taccatcata tgaatatagt agccctaaag cagggtaagc aatcctgttt ttgatttcca
     1261 atgtattaat ggttattggt gtgaaaagaa gttgatacat atagcctcct aaacataaca
     1321 ctgttatgtt tagatt
//
LOCUS       DAORYL010000238         1322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_69108_length_1322_cov_7.766377, whole genome shotgun sequence.
ACCESSION   DAORYL010000238 DAORYL010000000
VERSION     DAORYL010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69108_length_1322_cov_7.766377"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PLV81_16115"
     CDS             <1..218
                     /locus_tag="PLV81_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018401180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOM11668.1"
                     /translation="ILISAGFDAHNADPLSYIGLTSESYYKFTVLLKQVANEFCGGRM
                     IAFLEGGYDLEALAGSVDLMMRGFIED"
     gene            complement(215..1075)
                     /locus_tag="PLV81_16120"
     CDS             complement(215..1075)
                     /locus_tag="PLV81_16120"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOM11669.1"
                     /translation="MITPPTKGILYVLLSAITFSLSTVFAKYITSTSSIHGIEISFVR
                     FIVGFIISSGYVIIHKMSIRPNHIPLVITRAISNTIAVILFFVGVQYTTVTNANMLNM
                     TYLVFVFLLAPFFNKERIIKINFIYLLITLAGTYLVIMPHYGSVNIGDIYSFLSAIVA
                     AIAVVALRESRKYDSTSIILFHLMSIGLVINGIAMIPVFAIPDSVTLVYMLLSGLSAY
                     LGQAFFTAGFKFVDATSGSILSSSRIFIAACMGIYFFNDSFASNIIIGGLLITVALIG
                     VSLQENKDIH"
BASE COUNT          481 a          200 c          237 g          404 t
ORIGIN      
        1 ttatccttat ttcagctggt tttgatgctc acaatgccga ccccttatca tacattgggc
       61 ttacctctga gtcatactat aaatttactg tcctgttgaa gcaggtcgca aatgaatttt
      121 gtggtggcag aatgatagct ttccttgaag gtggctatga cctggaagca ctggcaggaa
      181 gtgttgattt aatgatgcga ggatttattg aagattaatg tatgtctttg ttttcctgaa
      241 gactcacacc tatcagagcc accgtaatga gcaacccgcc aataataata ttagaggcaa
      301 atgagtcgtt gaaaaaatat atacccatgc atgctgcaat aaatatcctg cttgaagaaa
      361 ggatagatcc gcttgttgca tcaacgaatt taaatccagc agtaaaaaaa gcctgtccta
      421 aatatgcact taaacctgaa agcagcatat aaactaatgt aacactatca ggaattgcaa
      481 ataccggtat catggcaatg ccatttataa caagtcctat actcataagg tgaaataata
      541 ttatggaagt actatcgtat tttcgggatt cccgaagggc aacaactgca attgctgcaa
      601 caatagctga aagaaatgag taaatatcac caatattaac agagccgtaa tgaggcataa
      661 tcactaaata ggtgcctgcc agtgttatta aaagatagat aaaattaatt tttattatgc
      721 gttctttatt aaagaatgga gcaagtaaaa atacaaaaac aagatatgtc atgtttagca
      781 tgtttgcatt ggtaacggtt gtatattgta caccaacaaa aaaaagtata actgcaatgg
      841 tgttggatat agcccttgtt attactaacg gtatatgatt gggtctaata ctcattttat
      901 ggatgataac ataccctgaa gagataataa acccaacgat aaatcttaca aaagaaatct
      961 caataccatg aatggaggat gtactggtga tatattttgc aaacaccgtt gataaagaaa
     1021 aagtaattgc agaaaggagt acatacagaa tgccttttgt aggaggtgta atcattgtat
     1081 aaattccata atttacgtat tatatgctga ttttaaaaaa tataaactaa aatttacata
     1141 tatgattatg caaatctatt aaaacatata gtagcacata atggcagata tgttggtaaa
     1201 tgatagtaaa aatattttat tattaattct gtaaaaaaag ttgtaatata acgtggtaat
     1261 gtgataaaat ctaaacataa cagtgttatg tttaggaggc tatatgtatc aacttctttt
     1321 ca
//
LOCUS       DAORYL010000239         1266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_73582_length_1266_cov_9.086705, whole genome shotgun sequence.
ACCESSION   DAORYL010000239 DAORYL010000000
VERSION     DAORYL010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73582_length_1266_cov_9.086705"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="PLV81_16125"
     CDS             <1..1229
                     /locus_tag="PLV81_16125"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOM11670.1"
                     /translation="INKTIGVIQLINSKEDIDHNYNGNEAYSITLTCEEDFNTKVVPF
                     QKRYESLMEAVASQAAIAIENNRMIRQIQQQFEEFVRASVTAIESRDVATSGHSFRVA
                     DMCLKMAQAINEETTGPFGTITFTDTELKELEYAALLHDFGKVYIDTAIFLKSHKLYP
                     KDLENILLKLEYLYRYQQLKYAMTIFNEMKKDDAFDKHLDKINAIEIERDDILRKILE
                     ARQKVIELNNPMVKEMDVEKEVKHLYAMLQSLDCCDVDNHHLEIFNDYYIKNLTIKRG
                     SLNDDERREIESHVVHTYNFVSKIPWPPEFARIPEIAAKHHEKLDGTGYPYGLKADQI
                     PLQARIMALADIFDALIATDRPYKPPITLEQALNIIKEEAEKNKLDKDIVDLFIKKKI
                     YEQIDRNAYRTKSTVH"
BASE COUNT          460 a          212 c          256 g          338 t
ORIGIN      
        1 atattaacaa aacgattggt gtaatacagc ttattaatag taaggaagac attgatcata
       61 attataatgg caatgaagcg tattcaatta cattaacctg tgaggaagat tttaacacaa
      121 aggtagttcc cttccagaaa cgatatgaaa gcttaatgga agcggtagca tctcaggctg
      181 ctattgccat agaaaacaac cgcatgatac ggcaaattca gcagcagttt gaagaatttg
      241 ttagggcatc ggttactgca attgaatcac gtgatgttgc cacatccggc cattcgttca
      301 gggttgctga tatgtgcttg aagatggcac aggcaattaa tgaagaaacc acaggaccat
      361 ttggcaccat aacatttacc gatactgaat tgaaagaact ggaatatgca gccctgttgc
      421 atgactttgg gaaggtatac attgacactg caatattttt aaaatcccat aaattgtacc
      481 caaaggattt agaaaatatt ttgctaaaac ttgaatatct ttatcggtat cagcaattga
      541 agtacgcaat gacaatattt aatgaaatga aaaaagatga tgcatttgat aaacaccttg
      601 acaagataaa tgcaatagaa attgagcggg atgatatttt acgcaagata cttgaagcac
      661 ggcaaaaggt gatagaactt aacaatccaa tggtaaagga aatggatgtt gaaaaagagg
      721 ttaaacattt atatgccatg ctgcagtcac ttgattgctg tgatgttgat aatcatcact
      781 tggaaatttt caatgattac tacataaaga atttaaccat caagcgtggc agcttaaacg
      841 atgatgagcg ccgtgaaata gaaagccatg tggtacatac gtataatttt gtaagcaaga
      901 tcccgtggcc gcctgagttt gcgcgaatac ctgaaattgc agcaaaacac cacgaaaaat
      961 tagatggcac ggggtaccct tatggattaa aggctgatca aattcctctg caggcacgca
     1021 ttatggcact ggcggatata tttgatgcat taattgccac tgacagacct tacaagccac
     1081 caataacgtt agaacaggca ctgaatataa taaaagaaga agctgaaaaa aataaattgg
     1141 ataaagatat tgtagatctt ttcataaaga aaaaaattta tgagcagatt gaccgcaatg
     1201 cataccgtac taaatccact gtgcattaaa taaaattttt ttacgcagat atcgcagagt
     1261 acggaa
//
LOCUS       DAORYL010000240         1211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_78411_length_1211_cov_11.467993, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000240 DAORYL010000000
VERSION     DAORYL010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78411_length_1211_cov_11.467993"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /gene="panB"
                     /locus_tag="PLV81_16130"
     CDS             complement(<1..613)
                     /gene="panB"
                     /locus_tag="PLV81_16130"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003249639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOM11671.1"
                     /translation="MSSIKNIKDFAKMKQSGEPIAMITCYDYAFARVVSETDIDVILV
                     GDTLGMVVAGFDTTIPVTLDQMIYHTSMVRRGAPQSFIVTDLPFMSYHVSIEDTMRNC
                     GRIMKECGANAVKLEGGRDFAPVVEALTRASIPVMGHLGLTPQSVHKLGGYSVQGKDE
                     EQRKIIMEDALLLQKAGAFALVLEMVPEQLAKEITEALDIPTIG"
     gene            complement(601..1062)
                     /gene="folK"
                     /locus_tag="PLV81_16135"
     CDS             complement(601..1062)
                     /gene="folK"
                     /locus_tag="PLV81_16135"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOM11672.1"
                     /translation="MEAYAYILLGSNMGDSYRYIDAAIEKLSTLEKTKIIQQSSVIVT
                     KPLEYTKQPDFVNTVVALLTQLPPHTLLEKLQKIEYSLGRERTIPKGPRTIDCDILLY
                     DDMVCNTPELTLPHPQIYTRPFAAQLLLEINNDIIDPLSHKPLREVVLCHQ"
BASE COUNT          308 a          260 c          264 g          379 t
ORIGIN      
        1 tgccaatggt gggtatatca agcgcttcag ttatttcttt tgcaagctgc tcggggacca
       61 tttctaacac aagagcaaat gctcccgctt tttgtaaaag cagggcatct tccatgatga
      121 ttttacgttg ctcttcatct ttcccctgaa cagaatatcc gcctaattta tgaactgact
      181 gcggtgttaa gcccagatgc cccataaccg gtattgaagc tctggtgagt gcttcaacaa
      241 caggggcaaa atctcttccg ccttctaatt taacggcatt ggcgccacat tctttcataa
      301 tcctgccgca gttgcgcatg gtatcttcaa ttgatacatg gtagctcata aatggaaggt
      361 cagttactat aaagctttgc ggtgcaccac ggcgtaccat gctggtgtgg taaatcatct
      421 ggtcaagggt tactggtatg gtggtatcaa agcccgcaac aaccatcccc aatgtatcgc
      481 caaccagtat gacatcaata tcagtttcgc tgacaacacg tgcaaaggca taatcataac
      541 aggttatcat ggcaatgggc tcgcctgact gtttcatctt tgcaaaatct ttgatgtttt
      601 ttattgatga catagtacta cctcccgtaa tggtttgtga gaaagcggat caatgatatc
      661 gttgtttatt tccagcaata gctgtgctgc aaatggtcgt gtgtagattt gtggatgagg
      721 cagggtaagt tccggtgtgt tacacaccat atcatcgtaa agcaaaatat cgcaatcaat
      781 agtgcggggg cctttaggga tggttctttc ccttcccagt gaatactcta ttttttgaag
      841 cttttcaagt agcgtgtgtg gtggcagttg cgtgagcagc gcaacaactg tattgacaaa
      901 atcgggctgc ttggtatatt ctaaaggttt agttactatt acactgcttt gttgtataat
      961 ttttgttttt tccagtgtgg acaacttttc aatagcagcg tcaatatacc ggtaactatc
     1021 gcccatattg cttcccagta aaatgtatgc atacgcttcc attataattt ttccctgtaa
     1081 tcgggacctg ttagctctaa aattgtgcac atttccttaa tgcgcgacaa tactctgccg
     1141 ccagcaattt cagacagtgc acgatggggg tcaacgttgg atgtaaatat ggtaaatttt
     1201 tcggcctcgt a
//
LOCUS       DAORYL010000241         1128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_86898_length_1128_cov_9.670084, whole genome shotgun sequence.
ACCESSION   DAORYL010000241 DAORYL010000000
VERSION     DAORYL010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86898_length_1128_cov_9.670084"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1128
                     /locus_tag="PLV81_16140"
     CDS             <1..>1128
                     /locus_tag="PLV81_16140"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOM11673.1"
                     /translation="GLQQDAIEVFDNALRQVHVCKSVCNDMIQDLQTLRLRSPLKQCL
                     PLLKALEEIIGVLEKVESEKLDEDMRLEYEYRKSIIVNTCTDLSMIVFMDDDSYVYWL
                     EKEKNAQGTVTMCGQPLDVASIIHKEVVEYYDHLAFVSATLSVNNTFDYFVQSIGLSN
                     VQTVLLPSPFDFASQSVLFIDKENVDPSEEAFINNAAEKIREIITMVQGRCLVLFTSY
                     IMLQKIYEKLCNTVPYTIFSQHMMNAATALQEYIACDNAVLFGTHSFWQGVDLPGDLA
                     RAVIIMKLPFEAPENPVVQAKIEKIQKEGGNPFMSYQVPYAVLLLKQGFGRLIRKSTD
                     KGIIAILDSRIVSKSYGRIFLASLPNSKRVYSLQELKKAYN"
BASE COUNT          355 a          200 c          249 g          324 t
ORIGIN      
        1 acgggctaca acaggatgcc attgaagtat ttgacaatgc attgcgacag gttcacgtat
       61 gcaaatctgt ctgtaatgat atgatacagg atttgcagac actaagactt agaagtcctt
      121 taaaacaatg cctgccatta ctgaaggctc tggaagagat tattggtgta cttgaaaaag
      181 tggaatccga aaaacttgat gaagacatgc gattggaata tgagtaccgc aaaagcatta
      241 ttgtgaatac atgtactgat ctgagtatga ttgtattcat ggatgatgac agttacgttt
      301 actggctgga aaaggaaaaa aatgctcagg gaacggtgac catgtgcggc cagccgctgg
      361 atgttgcttc cattattcat aaagaggtgg tggaatatta cgatcattta gcttttgttt
      421 cagcaacatt gagtgttaat aatacgtttg attactttgt gcagagtatt ggtctttcaa
      481 atgtgcaaac agtactattg ccatcaccat ttgattttgc cagtcaatcg gttttattta
      541 ttgataaaga gaatgttgac ccgtcagaag aagcttttat taacaatgca gctgaaaaaa
      601 tacgggaaat cattaccatg gtgcagggac gctgccttgt cctttttacg tcgtatataa
      661 tgctacaaaa gatatatgaa aaactttgta acacagtgcc atatactatt ttttcacaac
      721 acatgatgaa tgcggcaaca gctttacagg aatatatcgc ttgtgacaat gcagtgcttt
      781 ttggtacaca ttcattctgg cagggtgtgg atttgccagg ggatctggcc cgtgcagtca
      841 ttatcatgaa gttgccattt gaggcaccgg aaaatcctgt ggtgcaggca aagatagaaa
      901 aaatacaaaa agaagggggc aatccgttta tgagctatca ggttccctat gcggtgctcc
      961 tgttaaaaca gggctttggc agactcatcc gaaaatcaac tgataaaggt atcatagcaa
     1021 ttcttgactc ccgcatagtc agcaagtctt acggaagaat atttttagct tcactcccca
     1081 attcaaaaag ggtatactcg ctgcaagagt taaaaaaagc atataaca
//
LOCUS       DAORYL010000242         1128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_86899_length_1128_cov_9.530289, whole genome shotgun sequence.
ACCESSION   DAORYL010000242 DAORYL010000000
VERSION     DAORYL010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86899_length_1128_cov_9.530289"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1128
                     /locus_tag="PLV81_16145"
     CDS             <1..>1128
                     /locus_tag="PLV81_16145"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOM11674.1"
                     /translation="GLQQDAIEVFDNALRQVQICKSVCNDMIQDLQTLRLRNPLKQCL
                     PLLKALEEIIGVLEKVEDEKLDEDLRLEYEYRKSIIMNICNDLGMIVFMDDDSYVYWL
                     ERVKNGQGTVIMCGQPLDVASIIHKEVVEYYDHLAFVSATLSVNNTFDYFVQSIGLSN
                     VQTVLLPSPFDFASQSVLFIDKENVDPSEEAFINNAAEKIREIITMVQGRCLVLFTSY
                     IMLQKIYEKLCNTVPYTIFSQHMMNAATALQEYIACDNAVLFGTHSFWQGVDLPGDLA
                     RAVIIMKLPFEAPENPVVQAKIEKIQKEGGNPFMSYQVPNAVLLLKQGFGRLIRKSTD
                     KGIIAILDPRIVSKSYGRIFLASLPNSKRVYSLQELKKAYN"
BASE COUNT          357 a          198 c          247 g          326 t
ORIGIN      
        1 acgggctaca acaggatgcc attgaagtat ttgacaatgc attgcgacag gttcaaatat
       61 gcaaatctgt ctgcaatgat atgatacagg atctgcagac actcaggctg cgaaatcctt
      121 taaaacaatg cctgccattg ctgaaagctt tagaagagat tattggtgta cttgaaaaag
      181 tggaagacga aaaacttgat gaagacctgc gcttagaata tgagtatcgc aaaagcatta
      241 ttatgaatat atgcaatgat ttaggcatga ttgtatttat ggatgatgat agctatgttt
      301 actggctgga aagggtaaaa aatggtcagg gcacggttat tatgtgtggc cagccactgg
      361 atgttgcttc cattattcat aaagaggtgg tggaatatta cgatcattta gcttttgttt
      421 cagcaacatt gagtgttaat aatacgtttg attactttgt gcagagtatt ggtctttcaa
      481 atgtgcaaac agtactattg ccatcaccat ttgattttgc cagtcaatcg gttttattta
      541 ttgataaaga gaatgttgac ccgtcagaag aagcttttat taacaatgca gctgaaaaaa
      601 tacgggaaat cattaccatg gtgcagggac gctgccttgt cctttttacg tcgtatataa
      661 tgctacaaaa gatatatgaa aaactttgta acacagtgcc atatactatt ttttcacaac
      721 acatgatgaa tgcggcaaca gctttacagg aatatattgc atgtgacaat gcggtgcttt
      781 ttggtactca ttcattttgg cagggagtgg atttgccagg ggatttagcc cgtgcagtta
      841 ttattatgaa gctgccgttt gaggcaccgg aaaatccagt ggtgcaggca aagattgaaa
      901 aaatacaaaa agaaggcggc aacccgttca tgagttatca ggtccctaat gcggtgctcc
      961 tattaaaaca gggctttggc aggctcatcc gaaaatcaac cgataaaggc atcatagcaa
     1021 ttcttgaccc ccgcattgtc agcaaatcat acggaaggat atttttagca tccctcccca
     1081 attcaaaaag ggtatactcg ctgcaagagt taaaaaaagc atataaca
//
LOCUS       DAORYL010000243         1081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_92555_length_1081_cov_4.743665, whole genome shotgun sequence.
ACCESSION   DAORYL010000243 DAORYL010000000
VERSION     DAORYL010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92555_length_1081_cov_4.743665"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="PLV81_16150"
     CDS             <1..977
                     /locus_tag="PLV81_16150"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOM11675.1"
                     /translation="SFDFAIMRYEQQGKNIILSRRSLLDKIKEEAIEKLKQQLHSGDI
                     VQATVVQVLDAGLLVSLHSLDAFVPKSELSHSRKPNMNLYEPGQTITAKILDLDWNKV
                     KITLSIKQTLPEPWHYISQYQVGNTYTGTIAKIIKNGAFVEFDNGLEGFIPLSRMSYV
                     KKLHDPREVCNEGDSVQVKIIDIDQTSKKITLELITSEENPWLTVNESLRDSIQTATI
                     EEITAKGLVVVLPNQVRAFVPKEQCAIDRNADITKSYQVGATIKVVVTTVNADDRKCI
                     ASEKKAQEIEERQLYEKFIASNTSQGSSAFGQLFKQKYEEMQKKSKNS"
BASE COUNT          398 a          209 c          215 g          259 t
ORIGIN      
        1 aaagctttga ctttgccatt atgcgctatg agcagcaggg taaaaacatc attctatcac
       61 gaaggtcgct gcttgataaa atcaaagaag aagccataga aaagctgaaa cagcaattac
      121 acagtggcga tatagttcag gctactgttg ttcaggtact cgatgcaggc cttttagtat
      181 cgctacattc acttgatgcc tttgtgccaa aaagtgaact atcgcacagc cgtaagccaa
      241 acatgaatct gtatgaacca ggacagacta tcactgcaaa gatacttgat ttagactgga
      301 ataaagtcaa aataacattg agcattaagc aaacactgcc tgaaccatgg cattatataa
      361 gtcaatatca ggttggaaat acctatacag gaactatcgc caaaataatt aaaaatggtg
      421 catttgttga atttgataat ggccttgaag ggtttatccc actttcacgc atgagctatg
      481 tcaaaaagtt acatgacccc agggaagtat gtaatgaggg cgatagtgta caggttaaaa
      541 ttatagatat agaccaaacc agtaaaaaaa tcaccctgga acttatcacc agcgaagaaa
      601 atccctggct taccgtcaat gaatcactgc gtgattccat acaaactgca accattgaag
      661 aaattacagc aaaaggcctt gttgtggtgc tgccaaatca ggtgcgtgcg tttgttccca
      721 aagagcagtg tgcaattgac cgcaatgctg atataacaaa gtcatatcag gtgggagcaa
      781 ccatcaaagt tgtagtaact acagttaatg ccgatgacag aaaatgcatt gcttcagaaa
      841 aaaaagcaca ggaaattgaa gagcgccagt tatacgaaaa attcattgca tctaacacca
      901 gtcagggttc ttcagccttt gggcaactgt ttaaacagaa gtacgaggaa atgcagaaaa
      961 aatccaaaaa ctcataaacc tgtatggata ctaaaagtac acacagtgag caactggcat
     1021 atagagcata cgtagaaaac aatatccaat atattaacga aatccacaag aagattgaaa
     1081 a
//
LOCUS       DAORYL010000244         1063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_94800_length_1063_cov_11.663690, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000244 DAORYL010000000
VERSION     DAORYL010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94800_length_1063_cov_11.663690"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1063)
                     /locus_tag="PLV81_16155"
     CDS             complement(<1..>1063)
                     /locus_tag="PLV81_16155"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOM11676.1"
                     /translation="ENYSLTFRITRNCYWNKCAFCPVYKCGAKFSRRNIDEVLEDIKR
                     AKQIDDFLFEQGIGYPLYSDADFYKLQNILQKVQQQRWEAGIVDVNEDVSADADVKRI
                     DDERMQWFMQWFVEKPTLAQCFEHVVQWRIGGGKTCFLGDADGLIVAAKDYEKILSHI
                     KQNFPTLQRFTIYGRTKTARKKDFKDLVKLHKAGLHRVHFGLESGSDEVLKLMNKGED
                     SRDHIEGCLKTKEAGLSVSVYSMPGLGGKKLSHAHALETARVINATGPDFVRLRTLSI
                     FPYTPLMDMVKAGKFEPCSDDEIVDEIETLITAIDVPVMLYSDSATNLLPLNGKLPQD
                     KSTLLSAISEYKSKDYIER"
BASE COUNT          304 a          265 c          175 g          319 t
ORIGIN      
        1 aagcgctcaa tgtagtcctt agacttatat tcactgattg ctgaaagcaa tgtactttta
       61 tcctgcggca atttcccgtt taacggcaat agattcgttg cgctatcgct atacagcata
      121 acaggaacat cgattgctgt tatcagcgtt tcaatttcat ccacaatttc atcatcactg
      181 cacggctcaa acttacccgc tttgaccata tccattagcg gtgtatacgg aaaaatggaa
      241 agcgtacgca aacgtacaaa atcaggccct gttgcattga taacgcgcgc tgtttcaagc
      301 gcatgtgcat ggcttaactt tttacctccc aaaccaggca tactatatac cgaaaccgaa
      361 agccccgctt ctttggtctt taagcaaccc tcaatatggt cacgggaatc ttcaccctta
      421 ttcataagct tcaaaacctc atcgctacca ctttcaagac caaagtgcac acggtgtagc
      481 cccgctttgt gtaacttcac aagatcttta aaatcttttt tgcgcgcagt tttggtacgg
      541 ccatatatag tgaagcgttg caatgtaggg aaattttgtt ttatgtgcga taatatcttt
      601 tcatagtctt ttgctgctac tatcagtcca tcggcatcac ctaaaaagca tgttttacca
      661 ccaccaatgc gccattgcac aacatgttca aagcactgag ctaacgtcgg cttttcaaca
      721 aaccactgca taaaccattg catgcgttca tcatcaattc tttttacatc agcatcagca
      781 ctaacatctt cattaacatc aacaattcct gcttcccatc gttgttgctg aactttttga
      841 agtatattct gcaacttata aaagtcagca tcagaatata aaggataacc aattccctgc
      901 tcaaacagga aatcatcaat ttgctttgct cgctttatgt cttccagtac ttcgtcaata
      961 ttcctgcgcg aaaatttagc accacattta tacacaggac aaaacgcgca cttattccag
     1021 tagcagttac gggtaatcct gaacgtaagg ctgtaatttt ccg
//
LOCUS       DAORYL010000245         1063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_94801_length_1063_cov_9.935516, whole genome shotgun sequence.
ACCESSION   DAORYL010000245 DAORYL010000000
VERSION     DAORYL010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94801_length_1063_cov_9.935516"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1063)
                     /locus_tag="PLV81_16160"
     CDS             complement(<1..>1063)
                     /locus_tag="PLV81_16160"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOM11677.1"
                     /translation="ENYSLTFRITRNCYWNKCAFCPVYKCGAKFSRRNIDEVLEDIKQ
                     AKQIDDFLFEQGIGYPLYSDADFYKLQNILQKVQQQRWEAGIVDVNENVSADADVKRI
                     DDERMQWFMQWFVEKPTLAQCFEHVVQWRIGGGKTCFLGDADGLIVAAKDYEKILSHI
                     KQNFPTLQRFTIYGRTKTARKKDFKDLVKLHKAGLHRVHFGLESGSDEVLKLMNKGED
                     SRDHIEGCLKTKEAGLSVSVYSMPGLGGKKLSHAHALETARVINATWPDFVRLRTLSV
                     FPYTPLMDMVKAGEFEPCSDDEIVDEIETLITAIDVPVMLYSDSATNLLPLNGKLPQD
                     KRTLLSAISEYKSKDYIER"
BASE COUNT          305 a          263 c          178 g          317 t
ORIGIN      
        1 aagcgctcaa tgtagtcctt agacttatat tcactgattg ctgaaagcaa tgtacgttta
       61 tcctgcggca attttccgtt taacggtaat agattcgttg cgctatcgct atacagcata
      121 acaggaacat caatagctgt tatcagtgtt tcaatttcat ccacaatttc atcatcactg
      181 cacggctcaa actcacccgc tttgaccata tccattagcg gtgtatacgg aaaaacggaa
      241 agcgtacgca aacggacaaa atcaggccat gttgcattga taacgcgcgc agtttcaagc
      301 gcatgcgcat ggcttaactt tttacctccc aaaccaggca tactatatac cgaaaccgaa
      361 agccccgctt ctttggtctt taagcaaccc tcaatatggt cacgggaatc ttcaccctta
      421 ttcataagct tcaaaacctc atcgctacca ctttcaagac caaagtgcac acggtgtagc
      481 cccgctttgt gtaacttcac aagatcttta aaatcttttt tgcgcgcagt tttggtacgg
      541 ccatatatag tgaagcgttg caatgtaggg aaattttgtt ttatgtgcga taatatcttt
      601 tcatagtctt ttgctgctac tatcagtcca tcggcatcac ctaaaaagca tgttttacca
      661 ccaccaatgc gccattgcac aacatgctca aagcactgag ctaacgttgg cttttcaaca
      721 aaccactgca taaaccactg catgcgttca tcatcaattc tttttacatc agcatcagca
      781 ctaacatttt cattaacatc aacaattcct gcttcccagc gttgttgctg tactttttga
      841 agtatattct gcaacttata gaagtcagca tcagaatata atggataacc aattccctgc
      901 tcaaacagga aatcatcaat ttgctttgct tgctttatgt cttccagtac ttcgtcaata
      961 ttcctgcgcg aaaatttagc gccacattta tacacaggac aaaacgcaca cttattccag
     1021 tagcagttac gggtaatcct gaacgtaagg ctgtaatttt ccg
//
LOCUS       DAORYL010000246         1062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_94971_length_1062_cov_3.096326, whole genome shotgun sequence.
ACCESSION   DAORYL010000246 DAORYL010000000
VERSION     DAORYL010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94971_length_1062_cov_3.096326"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..741)
                     /locus_tag="PLV81_16165"
     CDS             complement(172..741)
                     /locus_tag="PLV81_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11678.1"
                     /translation="MKLKLYCAFIACIMLCVCTDVFAQRKKADYFLKPQVGIWFGPVT
                     PVFSTGDDVDTALWGGGFVRFNVFSLPLKVGFDSSYEHFKSEGVKELTVIPVYGNVLW
                     RLPIDMPIAFQLKAGAGSAKVTIEPEKSSQWDPMGMLGFELSFPAGKWINIGLRIDYL
                     FIYEGYIEGARKNGHIINTGLTLYLNIGI"
     gene            complement(875..>1062)
                     /locus_tag="PLV81_16170"
     CDS             complement(875..>1062)
                     /locus_tag="PLV81_16170"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11679.1"
                     /translation="HNMVRIIVGTILYLLHKGKSPETMKDIIESCDRNCSGPTAPAYG
                     LYFKKVYNPEAYNFLNV"
BASE COUNT          321 a          245 c          169 g          327 t
ORIGIN      
        1 ccataaactc acgctaccac tatggtctgt agactcaaat gtgcccttga ttatcacttt
       61 caggcggctt ccagatagct catcaaacag tggtttgttg ccagtacacc cgcttacaag
      121 aactatcgcc acaaaacata ctatgtaatg taaactttta ttcatactac attatatccc
      181 aatattaaga taaagtgtta aaccagtatt aataatatgc ccgtttttcc gtgcaccttc
      241 aatgtatcct tcatatataa aaagataatc aatcctgagg ccaatattaa tccatttgcc
      301 cgctggaaac gacagctcaa agccaagcat ccccatggga tcccactggc ttgacttttc
      361 aggctctatg gttaccttag cactacctgc ccctgctttt aattgaaagg caataggcat
      421 atcaattggc agtctccaga gaacattgcc atataccggt attaccgtaa gctcttttac
      481 gccttcagat ttaaaatgct cgtacgaaga atcaaaacca acttttaatg gtaatgagaa
      541 tacattaaag cgcacaaacc cgccacccca cagtgctgta tcaacatcat cgccagtgga
      601 aaacaccggt gttaccggac caaaccatat cccaacctgc ggttttagga aataatcagc
      661 ctttttacgc tgtgcaaaaa catcagtgca cacacaaagc atgatacagg caatgaaggc
      721 acaatatagt ttcaatttca cggtattcat ccttcttttt ttcaattacc attgtttttg
      781 tgtagtaatg tgtcaattat tattttttta tagcatcagc atagagtacc tggcaaatgc
      841 tcatatgtaa tacgaaccaa tgtaattatt tttgttacac gtttaaaaaa ttgtacgctt
      901 ctggattata caccttttta aaatataatc cgtatgcagg tgcagttggt ccactgcagt
      961 ttctatcgca gctttcaata atatctttca ttgtttccgg gcttttgccc ttgtgtagca
     1021 ggtacagtat ggtccctaca ataatccgta ccatattatg ta
//
LOCUS       DAORYL010000247         1018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_101311_length_1018_cov_14.058152, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000247 DAORYL010000000
VERSION     DAORYL010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101311_length_1018_cov_14.058152"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PLV81_16175"
     CDS             <1..416
                     /locus_tag="PLV81_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11680.1"
                     /translation="IGAPYYDINADDDKGCFYVYQKSQSSYTLVSQVKNISGTNGDAL
                     GMAVSILPDGQYMFASMPLADEGGVDRGRVLVYRLNGSNVEYMDSFYPDDRNEKCYFG
                     YSLAVSSTGVVAIGAPYAFTHATNDNGGVVYIRRL"
     gene            510..>1018
                     /locus_tag="PLV81_16180"
     CDS             510..>1018
                     /locus_tag="PLV81_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11681.1"
                     /translation="MVQHVIKAIIALITVLMTTTMAVAQLSVSQPEVEIGHTVATSRF
                     IGMMGGMYGYAYPAGSIYTSDDYVKRQKGKYFGATHGIGYELSPHLILHGGFTLWDME
                     VFITRHLFMQDMKTRYSLDTCALHTGLMFIYKWFFADAIVYTELPLEDDTERVELNGV
                     YMQTNTLKQ"
BASE COUNT          315 a          160 c          246 g          297 t
ORIGIN      
        1 tcattggtgc accatactat gatataaatg cagatgatga taaaggatgc ttttatgtat
       61 atcaaaaaag tcagagcagc tatacattag tttcacaggt taaaaatatt agtggaacaa
      121 atggtgatgc actggggatg gcggtaagta ttttgccaga tgggcagtat atgtttgcat
      181 ctatgcctct tgctgatgaa ggtggggttg atagaggcag ggttttagtg tatagactca
      241 atggatcaaa tgttgaatat atggattctt tttatcctga tgacagaaat gaaaagtgtt
      301 attttggcta ttcacttgca gtaagcagta ccggtgttgt tgccattggg gcaccgtatg
      361 catttacaca tgcaaccaat gataatgggg gagtggtata tatacgcagg ttgtaatgat
      421 ggtgtttgtt gtaatcattt taaacccaac agggcgggaa gtaaaaatga caatacaata
      481 gaaatctttg ctaaaaggga accgtggtaa tggtacagca tgtaataaaa gcaataatag
      541 cactaataac agtattgatg acaaccacaa tggcagtagc acagctttca gtatcgcaac
      601 cggaggttga aatcggtcat actgtggcta ccagtagatt tattggaatg atgggaggga
      661 tgtacggcta tgcatatcct gctggctcaa tttatacgtc agacgattat gtgaagcgcc
      721 agaagggaaa atattttggg gcaacccatg gaattggcta tgaactatcg ccccacctta
      781 tactgcatgg cggatttact ctgtgggaca tggaagtttt tattacacgg catcttttta
      841 tgcaggacat gaaaacgcgt tacagccttg atacctgtgc actgcataca ggtttaatgt
      901 ttatatataa atggtttttt gctgatgcaa ttgtttatac ggagctacca cttgaagatg
      961 acacggaacg ggtggaactt aacggagtgt atatgcaaac caatacatta aaacagca
//
LOCUS       DAORYL010000248         1016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1962
            NODE_101611_length_1016_cov_8.074922, whole genome shotgun
            sequence.
ACCESSION   DAORYL010000248 DAORYL010000000
VERSION     DAORYL010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863491
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:45:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,168
            CDSs (with protein)               :: 3,168
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101611_length_1016_cov_8.074922"
                     /isolate="AMR_MDS_1962"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1016)
                     /locus_tag="PLV81_16185"
     CDS             complement(<1..>1016)
                     /locus_tag="PLV81_16185"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM11682.1"
                     /translation="GCSDINYPDNVLDKDPNDMSGGSIVIDGGAPYAYSQQVTISCMV
                     DNAVEMQFSQDLITWSTLEVFSNTRVWILPLEYGQQSVYGQFFNSRGDAVMYDDSIFF
                     IERCIASTSAYLGGDVAISDDGTVIAAGSYEGNANAVYVFRKQTIDWDCTVIKPDDIS
                     TGDKFGYSVALSGDGTWLFVGAPVKRAVYVYEYTGSWHLRQTITSTADHFGHAIATSY
                     DGQYITLASASSVFAYKYNSNTNQYGSFTVNGSDLINVCNVMISNGGVWIFASYYNTN
                     TGGVMSFVRTDDYFGNPKSIKPDDYQPYSYFGKSIALSSDSNYLIIGAPYYDINADDD
                     KGCF"
BASE COUNT          322 a          240 c          159 g          295 t
ORIGIN      
        1 taaaagcatc ctttatcatc atctgcattt atatcatagt atggtgcacc aatgattaaa
       61 taattactat ctgaagaaag agctattgat ttcccaaaat atgaataagg ttgataatca
      121 tcaggtttaa tggatttggg atttccaaaa taatcatctg ttcgtacaaa tgacatcaca
      181 ccacctgtat tggtattgta atacgaagca aaaatccata ccccaccatt acttatcata
      241 acattgcata cattaatcag atctgatcca ttgacagtga atgatccata ttgattagtg
      301 ttgctattgt atttataggc aaatacactg cttgcagatg ccaaagtaat atactgccca
      361 tcataacttg ttgcaatcgc atgcccaaaa tgatctgcag ttgaggtaat cgtttgtctc
      421 aagtgccagc tgcctgtgta ctcatacaca tacactgccc ttttaaccgg tgcacctaca
      481 aaaagccatg taccatcacc actcaaggca acgctatatc caaatttatc acctgtgcta
      541 atgtcatcag gtttaatcac cgtacagtcc caatcaattg tctgttttct gaatacgtat
      601 accgcattgg cattgccttc atagcttcca gcagcaataa ctgtaccatc atccgaaata
      661 gccacatcac caccaaggta tgcagaggta cttgctatac agcgctcaat gaaaaatata
      721 gaatcatcat acataaccgc atcaccacgg ctgttaaaaa actgcccgta tactgattgt
      781 tgtccatact ctaaaggaag tatccacact ctggtattgc taaacacttc cagtgtgctc
      841 caggttatta aatcctgtga aaattgcatc tctacagcgt tatcaaccat gcaggaaatg
      901 gtaacctgct gcgaatatgc atacggcgcc ccaccatcaa taacaatact gccaccactc
      961 atgtcattgg gatctttatc cagtacattg tcaggataat taatgtcact gcatcc
//
